LOCUS       DAJTSL010000001        89995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTSL010000001 DAJTSL010000000
VERSION     DAJTSL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 89995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.001"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..791
                     /locus_tag="OO433_000001"
     CDS             <1..791
                     /locus_tag="OO433_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6048631.1"
                     /translation="DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQT
                     GVCQGDTSRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGVWVDGMGSPSTGD
                     FHDLVKLSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITY
                     DCGDTSKIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQ
                     NGILPVDPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(834..1694)
                     /gene="rsmI"
                     /locus_tag="OO433_000002"
     CDS             complement(834..1694)
                     /gene="rsmI"
                     /locus_tag="OO433_000002"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6048632.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            1758..3785
                     /gene="lpoA"
                     /locus_tag="OO433_000003"
     CDS             1758..3785
                     /gene="lpoA"
                     /locus_tag="OO433_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6048633.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQPVP
                     VSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEEL
                     LKSNTPLNVLVLNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSS
                     LGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATTDSG
                     MTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAMLYAS
                     SRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDA
                     WSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3743..4138
                     /locus_tag="OO433_000004"
     CDS             3743..4138
                     /locus_tag="OO433_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6048634.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGWTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4158..4748
                     /gene="diaA"
                     /locus_tag="OO433_000005"
     CDS             4158..4748
                     /gene="diaA"
                     /locus_tag="OO433_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6048635.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4758..5333
                     /gene="yraP"
                     /locus_tag="OO433_000006"
     CDS             4758..5333
                     /gene="yraP"
                     /locus_tag="OO433_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6048636.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5453..6004)
                     /locus_tag="OO433_000007"
                     /pseudo
     CDS             complement(5453..6004)
                     /locus_tag="OO433_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            complement(6077..6712)
                     /locus_tag="OO433_000008"
     CDS             complement(6077..6712)
                     /locus_tag="OO433_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6048637.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6840..7358
                     /gene="yhbO"
                     /locus_tag="OO433_000009"
     CDS             6840..7358
                     /gene="yhbO"
                     /locus_tag="OO433_000009"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6048638.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7338..7781)
                     /locus_tag="OO433_000010"
     CDS             complement(7338..7781)
                     /locus_tag="OO433_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6048639.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     HRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7832..8134
                     /gene="yhbQ"
                     /locus_tag="OO433_000011"
     CDS             7832..8134
                     /gene="yhbQ"
                     /locus_tag="OO433_000011"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6048640.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8121..8624)
                     /locus_tag="OO433_000012"
     CDS             complement(8121..8624)
                     /locus_tag="OO433_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6048641.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8618..9142)
                     /locus_tag="OO433_000013"
     CDS             complement(8618..9142)
                     /locus_tag="OO433_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6048642.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADVSDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLHVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9351..10331
                     /locus_tag="OO433_000014"
     CDS             9351..10331
                     /locus_tag="OO433_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6048643.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPNIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10340..11218
                     /locus_tag="OO433_000015"
     CDS             10340..11218
                     /locus_tag="OO433_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6048644.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            11424..12431
                     /locus_tag="OO433_000016"
     CDS             11424..12431
                     /locus_tag="OO433_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR6048645.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12549..13793)
                     /gene="mtr"
                     /locus_tag="OO433_000017"
     CDS             complement(12549..13793)
                     /gene="mtr"
                     /locus_tag="OO433_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6048646.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13947..15836)
                     /gene="deaD"
                     /locus_tag="OO433_000018"
     CDS             complement(13947..15836)
                     /gene="deaD"
                     /locus_tag="OO433_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6048647.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15829..15909)
                     /gene="yrbN"
                     /locus_tag="OO433_000019"
     CDS             complement(15829..15909)
                     /gene="yrbN"
                     /locus_tag="OO433_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6048648.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16016..16900)
                     /gene="nlpI"
                     /locus_tag="OO433_000020"
     CDS             complement(16016..16900)
                     /gene="nlpI"
                     /locus_tag="OO433_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6048649.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDSDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17009..19144)
                     /gene="pnp"
                     /locus_tag="OO433_000021"
     CDS             complement(17009..19144)
                     /gene="pnp"
                     /locus_tag="OO433_000021"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6048650.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRSVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19392..19661)
                     /gene="rpsO"
                     /locus_tag="OO433_000022"
     CDS             complement(19392..19661)
                     /gene="rpsO"
                     /locus_tag="OO433_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6048651.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19810..20754)
                     /gene="truB"
                     /locus_tag="OO433_000023"
     CDS             complement(19810..20754)
                     /gene="truB"
                     /locus_tag="OO433_000023"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6048652.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICFGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITIYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20754..21155)
                     /gene="rbfA"
                     /locus_tag="OO433_000024"
     CDS             complement(20754..21155)
                     /gene="rbfA"
                     /locus_tag="OO433_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6048653.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21319..24015)
                     /gene="infB"
                     /locus_tag="OO433_000025"
     CDS             complement(21319..24015)
                     /gene="infB"
                     /locus_tag="OO433_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6048654.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREA
                     AEKDKVSNQQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMA
                     EENKWTDNAEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKH
                     AESKADREEARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMA
                     VKGSQVIKAMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTG
                     AAAEPRAPVVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITF
                     LDTPGHAAFTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKI
                     DKPEADPDRVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELK
                     AVRKGMASGAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQE
                     VLEAGPSIPVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKL
                     ENMFANMTEGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETD
                     ATLAAASNAILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPEL
                     KQQIIGLAEVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRF
                     KDDVNEVRNGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24040..25527)
                     /gene="nusA"
                     /locus_tag="OO433_000026"
     CDS             complement(24040..25527)
                     /gene="nusA"
                     /locus_tag="OO433_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6048655.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLELGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGVTDEKAGALIMAARNICWFGDEA"
     gene            complement(25555..26007)
                     /gene="rimP"
                     /locus_tag="OO433_000027"
     CDS             complement(25555..26007)
                     /gene="rimP"
                     /locus_tag="OO433_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6048656.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGHTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26214..26290)
                     /locus_tag="OO433_000028"
     tRNA            complement(26214..26290)
                     /locus_tag="OO433_000028"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26254..26256),aa:Met,seq:cat)
     gene            26638..27981
                     /gene="argG"
                     /locus_tag="OO433_000029"
     CDS             26638..27981
                     /gene="argG"
                     /locus_tag="OO433_000029"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6048657.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSNDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(27937..29613)
                     /locus_tag="OO433_000030"
     CDS             complement(27937..29613)
                     /locus_tag="OO433_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6048658.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFWAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKTFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMFRKEFTEDKQGNPPLKGRVDVELLAFVFHILHLRHA"
     gene            complement(30072..30158)
                     /locus_tag="OO433_000031"
     tRNA            complement(30072..30158)
                     /locus_tag="OO433_000031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30122..30124),aa:Leu,seq:gag)
     gene            complement(30173..30505)
                     /gene="secG"
                     /locus_tag="OO433_000032"
     CDS             complement(30173..30505)
                     /gene="secG"
                     /locus_tag="OO433_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6048659.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30733..32070)
                     /gene="glmM"
                     /locus_tag="OO433_000033"
     CDS             complement(30733..32070)
                     /gene="glmM"
                     /locus_tag="OO433_000033"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6048660.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32063..32911)
                     /gene="folP"
                     /locus_tag="OO433_000034"
     CDS             complement(32063..32911)
                     /gene="folP"
                     /locus_tag="OO433_000034"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6048661.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWLSVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(33001..34935)
                     /gene="ftsH"
                     /locus_tag="OO433_000035"
     CDS             complement(33001..34935)
                     /gene="ftsH"
                     /locus_tag="OO433_000035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6048662.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMIEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35035..35664)
                     /gene="rlmE"
                     /locus_tag="OO433_000036"
     CDS             complement(35035..35664)
                     /gene="rlmE"
                     /locus_tag="OO433_000036"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6048663.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35790..36083
                     /gene="yhbY"
                     /locus_tag="OO433_000037"
     CDS             35790..36083
                     /gene="yhbY"
                     /locus_tag="OO433_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6048664.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36240..36716)
                     /gene="greA"
                     /locus_tag="OO433_000038"
     CDS             complement(36240..36716)
                     /gene="greA"
                     /locus_tag="OO433_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6048665.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36964..38397
                     /gene="dacB"
                     /locus_tag="OO433_000039"
     CDS             36964..38397
                     /gene="dacB"
                     /locus_tag="OO433_000039"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6048666.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMIATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVIPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38437..39609)
                     /gene="cgtA"
                     /locus_tag="OO433_000040"
     CDS             complement(38437..39609)
                     /gene="cgtA"
                     /locus_tag="OO433_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6048667.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39625..40590)
                     /locus_tag="OO433_000041"
     CDS             complement(39625..40590)
                     /locus_tag="OO433_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6048668.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40717..40974)
                     /gene="rpmA"
                     /locus_tag="OO433_000042"
     CDS             complement(40717..40974)
                     /gene="rpmA"
                     /locus_tag="OO433_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6048669.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40995..41306)
                     /gene="rplU"
                     /locus_tag="OO433_000043"
     CDS             complement(40995..41306)
                     /gene="rplU"
                     /locus_tag="OO433_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6048670.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41565..42536
                     /gene="ispB"
                     /locus_tag="OO433_000044"
     CDS             41565..42536
                     /gene="ispB"
                     /locus_tag="OO433_000044"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6048671.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42764..43042
                     /gene="sfsB"
                     /locus_tag="OO433_000045"
     CDS             42764..43042
                     /gene="sfsB"
                     /locus_tag="OO433_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6048672.1"
                     /translation="MESNFIDWHPPDIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43090..44349)
                     /gene="murA"
                     /locus_tag="OO433_000046"
     CDS             complement(43090..44349)
                     /gene="murA"
                     /locus_tag="OO433_000046"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6048673.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIKRVKGE"
     gene            complement(44404..44658)
                     /gene="ibaG"
                     /locus_tag="OO433_000047"
     CDS             complement(44404..44658)
                     /gene="ibaG"
                     /locus_tag="OO433_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6048674.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44818..45111)
                     /gene="mlaB"
                     /locus_tag="OO433_000048"
     CDS             complement(44818..45111)
                     /gene="mlaB"
                     /locus_tag="OO433_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6048675.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45111..45746)
                     /gene="mlaC"
                     /locus_tag="OO433_000049"
     CDS             complement(45111..45746)
                     /gene="mlaC"
                     /locus_tag="OO433_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6048676.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45765..46316)
                     /gene="mlaD"
                     /locus_tag="OO433_000050"
     CDS             complement(45765..46316)
                     /gene="mlaD"
                     /locus_tag="OO433_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6048677.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46321..47103)
                     /gene="mlaE"
                     /locus_tag="OO433_000051"
     CDS             complement(46321..47103)
                     /gene="mlaE"
                     /locus_tag="OO433_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6048678.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47111..47920)
                     /gene="mlaF"
                     /locus_tag="OO433_000052"
     CDS             complement(47111..47920)
                     /gene="mlaF"
                     /locus_tag="OO433_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6048679.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGCAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48130..49107
                     /locus_tag="OO433_000053"
     CDS             48130..49107
                     /locus_tag="OO433_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6048680.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49121..50107
                     /gene="kdsD"
                     /locus_tag="OO433_000054"
     CDS             49121..50107
                     /gene="kdsD"
                     /locus_tag="OO433_000054"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6048681.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50128..50694
                     /gene="kdsC"
                     /locus_tag="OO433_000055"
     CDS             50128..50694
                     /gene="kdsC"
                     /locus_tag="OO433_000055"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6048682.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSELLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50691..51266
                     /gene="lptC"
                     /locus_tag="OO433_000056"
     CDS             50691..51266
                     /gene="lptC"
                     /locus_tag="OO433_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6048683.1"
                     /translation="MSKARRWVTIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51235..51792
                     /gene="lptA"
                     /locus_tag="OO433_000057"
     CDS             51235..51792
                     /gene="lptA"
                     /locus_tag="OO433_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6048684.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51799..52524
                     /gene="lptB"
                     /locus_tag="OO433_000058"
     CDS             51799..52524
                     /gene="lptB"
                     /locus_tag="OO433_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6048685.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52572..54005
                     /gene="rpoN"
                     /locus_tag="OO433_000059"
     CDS             52572..54005
                     /gene="rpoN"
                     /locus_tag="OO433_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6048686.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTSETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            54028..54315
                     /gene="hpf"
                     /locus_tag="OO433_000060"
     CDS             54028..54315
                     /gene="hpf"
                     /locus_tag="OO433_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6048687.1"
                     /translation="MQLNITGNNVEITEALREFVRAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54433..54924
                     /gene="ptsN"
                     /locus_tag="OO433_000061"
     CDS             54433..54924
                     /gene="ptsN"
                     /locus_tag="OO433_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6048688.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54970..55824
                     /gene="rapZ"
                     /locus_tag="OO433_000062"
     CDS             54970..55824
                     /gene="rapZ"
                     /locus_tag="OO433_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6048689.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55821..56093
                     /gene="npr"
                     /locus_tag="OO433_000063"
     CDS             55821..56093
                     /gene="npr"
                     /locus_tag="OO433_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6048690.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56307..56939
                     /gene="yrbL"
                     /locus_tag="OO433_000064"
     CDS             56307..56939
                     /gene="yrbL"
                     /locus_tag="OO433_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6048691.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAYLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHHISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56936..57664)
                     /gene="mtgA"
                     /locus_tag="OO433_000065"
     CDS             complement(56936..57664)
                     /gene="mtgA"
                     /locus_tag="OO433_000065"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR6048692.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHRGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57661..58314)
                     /gene="elbB"
                     /locus_tag="OO433_000066"
     CDS             complement(57661..58314)
                     /gene="elbB"
                     /locus_tag="OO433_000066"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6048693.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58544..60880)
                     /gene="arcB"
                     /locus_tag="OO433_000067"
     CDS             complement(58544..60880)
                     /gene="arcB"
                     /locus_tag="OO433_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6048694.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60976..61905)
                     /locus_tag="OO433_000068"
     CDS             complement(60976..61905)
                     /locus_tag="OO433_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6048695.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62580..67040
                     /gene="gltB"
                     /locus_tag="OO433_000069"
     CDS             62580..67040
                     /gene="gltB"
                     /locus_tag="OO433_000069"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6048696.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLNVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            67053..68471
                     /gene="gltD"
                     /locus_tag="OO433_000070"
     CDS             67053..68471
                     /gene="gltD"
                     /locus_tag="OO433_000070"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6048697.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEQFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENKDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68655..69134
                     /locus_tag="OO433_000071"
     CDS             68655..69134
                     /locus_tag="OO433_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR6048698.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            69151..69456
                     /locus_tag="OO433_000072"
     CDS             69151..69456
                     /locus_tag="OO433_000072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6048699.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(69532..69996)
                     /locus_tag="OO433_000073"
     CDS             complement(69532..69996)
                     /locus_tag="OO433_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6048700.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPSEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(69993..70868)
                     /gene="nanK"
                     /locus_tag="OO433_000074"
     CDS             complement(69993..70868)
                     /gene="nanK"
                     /locus_tag="OO433_000074"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6048701.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LAALVAPLQAHAQQVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLSNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(70865..71554)
                     /locus_tag="OO433_000075"
     CDS             complement(70865..71554)
                     /locus_tag="OO433_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6048702.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(71602..73092)
                     /gene="nanT"
                     /locus_tag="OO433_000076"
     CDS             complement(71602..73092)
                     /gene="nanT"
                     /locus_tag="OO433_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6048703.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVSGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(73201..74094)
                     /gene="nanA"
                     /locus_tag="OO433_000077"
     CDS             complement(73201..74094)
                     /gene="nanA"
                     /locus_tag="OO433_000077"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6048704.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(74216..75007)
                     /gene="nanR"
                     /locus_tag="OO433_000078"
     CDS             complement(74216..75007)
                     /gene="nanR"
                     /locus_tag="OO433_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6048705.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            75387..76753
                     /gene="dcuC"
                     /locus_tag="OO433_000079"
                     /pseudo
     CDS             75387..76753
                     /gene="dcuC"
                     /locus_tag="OO433_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(76796..77293)
                     /gene="sspB"
                     /locus_tag="OO433_000080"
     CDS             complement(76796..77293)
                     /gene="sspB"
                     /locus_tag="OO433_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6048706.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(77299..77937)
                     /gene="sspA"
                     /locus_tag="OO433_000081"
     CDS             complement(77299..77937)
                     /gene="sspA"
                     /locus_tag="OO433_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6048707.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(78332..78724)
                     /gene="rpsI"
                     /locus_tag="OO433_000082"
     CDS             complement(78332..78724)
                     /gene="rpsI"
                     /locus_tag="OO433_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6048708.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(78740..79168)
                     /gene="rplM"
                     /locus_tag="OO433_000083"
     CDS             complement(78740..79168)
                     /gene="rplM"
                     /locus_tag="OO433_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6048709.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKCTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(79386..80513)
                     /gene="zapE"
                     /locus_tag="OO433_000084"
     CDS             complement(79386..80513)
                     /gene="zapE"
                     /locus_tag="OO433_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6048710.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            80707..81105
                     /locus_tag="OO433_000085"
     CDS             80707..81105
                     /locus_tag="OO433_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6048711.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            81259..82626
                     /gene="degQ"
                     /locus_tag="OO433_000086"
     CDS             81259..82626
                     /gene="degQ"
                     /locus_tag="OO433_000086"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR6048712.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDCGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            82716..83783
                     /gene="degS"
                     /locus_tag="OO433_000087"
     CDS             82716..83783
                     /gene="degS"
                     /locus_tag="OO433_000087"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6048713.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGVNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(83846..84784)
                     /gene="mdh"
                     /locus_tag="OO433_000088"
     CDS             complement(83846..84784)
                     /gene="mdh"
                     /locus_tag="OO433_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6048714.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPSEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            85219..85689
                     /gene="argR"
                     /locus_tag="OO433_000089"
     CDS             85219..85689
                     /gene="argR"
                     /locus_tag="OO433_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6048715.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            86054..86317
                     /gene="yhcN"
                     /locus_tag="OO433_000090"
     CDS             86054..86317
                     /gene="yhcN"
                     /locus_tag="OO433_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6048716.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(86372..86644)
                     /locus_tag="OO433_000091"
     CDS             complement(86372..86644)
                     /locus_tag="OO433_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6048717.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(86736..88703)
                     /gene="aaeB"
                     /locus_tag="OO433_000092"
     CDS             complement(86736..88703)
                     /gene="aaeB"
                     /locus_tag="OO433_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6048718.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IIADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQQRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTERVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(88709..89641)
                     /gene="aaeA"
                     /locus_tag="OO433_000093"
                     /pseudo
     CDS             complement(88709..89641)
                     /gene="aaeA"
                     /locus_tag="OO433_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
     gene            complement(89649..89852)
                     /locus_tag="OO433_000094"
     CDS             complement(89649..89852)
                     /locus_tag="OO433_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6048719.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
BASE COUNT        22132 a        24162 c        22361 g        21340 t
ORIGIN      
        1 atgacagcct ggatattaaa accacattac aggcaaacga cattccagga ctcacaaccg
       61 accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc gtatattctg
      121 ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc gttaatattg
      181 gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg atgaccattc
      241 cgaaaacgga tattgccgtc attaaaggcg tgtgggtcga tggaatggga agcccgtcta
      301 caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc gccccacagt
      361 cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc aatggtcaga
      421 agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt gatatcactt
      481 atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc gacggtacaa
      541 ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac aatgtgcccg
      601 atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct tttcagaacg
      661 gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc tggccagtta
      721 atctggtcgg tggtgagctg gaaacaggaa aatttcaggg cacagccacc attaccgtca
      781 tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca ctgttacccc
      841 tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat ttctgcgacc
      901 agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt gcgcagggca
      961 tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac catttcgcct
     1021 ttgcgacggt tttcatcttc ctttacccac gccagcagtt cgccaacggg cgcgccgtga
     1081 atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga ttcgcctaat
     1141 accgcaacga tatcttccag gctatctaac agacggtggg tggattcata aaaaatcagc
     1201 gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga tttggcaggt
     1261 aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa cgcagtgata
     1321 gcagcacacg gcccgggtag cggcaccacg cgaatccccg cttcacggca ggtacgcacc
     1381 agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag cgcaatgttt
     1441 tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt atggtcgtgc
     1501 agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt gtgacgagta
     1561 tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg ggtgatatcc
     1621 gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt atccgccgat
     1681 tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt gcgtaaaaaa
     1741 atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc gcgcgttgtc
     1801 tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact cccgatcagt
     1861 ccactgctta tatgcagggc acagcgcagg ctgattctgc cttttatctt cagcagatgc
     1921 agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt gcactggtga
     1981 aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa gaactgaacg
     2041 attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg cagaaagatt
     2101 ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa caaaaccagc
     2161 aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc attgatttac
     2221 tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag cagaatattg
     2281 atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg ctggtgatca
     2341 acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg tttgataacc
     2401 gtaacgatcc cgatatgatg aaagccggga tcgccgactg gcagaaacgt tatccgaaca
     2461 atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt aaaccagcct
     2521 cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta tttggtcgca
     2581 ctattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg gcggctcagg
     2641 tagctgccgc agacgtagca gaacaacctc agccgcaaac tgcggatggc gttgccagcc
     2701 cggcacaagc ctcggttagc gatctgaccg gtgatcagcc tgcggcccag ccggtgcctg
     2761 taagcgcccc ggcgacaagc accgcagcgg taagcgcacc cgcaaatcca tccgcagagc
     2821 tgaaaatcta cgatacctca tcacaaccac ttagccagat cttaagccag gttcagcagg
     2881 atggcgcgag tattgtggtt ggtccgttgc tgaaaaataa cgttgaagag ttgcttaaga
     2941 gcaacacccc gctgaacgtg ctggtactga accagccgga gaatatcgaa aaccgcgtca
     3001 atatttgtta cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg cgtcatattc
     3061 gtgaccaggg taaacaagcg ccgctggtgc tgatcccacg cagttcattg ggcgatcgcg
     3121 tagccaatgc gtttgcgcag gagtggcaga aactgggcgg cggcaccgtt ctgcaacaaa
     3181 aatttggttc caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt atcgctttaa
     3241 cgggtacccc gattactccc agagcgacaa ccgactccgg catgacgacc aacaatccaa
     3301 cgctgcaaac cacgccaacc gatgaccagt tcaccaataa tggcggtcgt gtcgatgcgg
     3361 tgtacattgt ggcaacgccg ggtgaaatcg cttttatcaa accgatgatc gccatgcgta
     3421 acggtagcca gagcggtgca atgctgtacg ccagctcccg cagcgcacaa ggcaccgcag
     3481 gcccggattt ccgtctggag atggaaggtt tgcagtacag cgaaatcccg atgctggcgg
     3541 gcggtaatct gccgttaatg cagcaggcac tcagcgcggt gaataacgat tattcactgg
     3601 ctcgcatgta tgcgatgggc gtcgatgcct ggtcgctggc aaatcatttc tcacaaatgc
     3661 gccaggttca gggttttgaa atcaacggta ataccggaag cctgacggca aacccggatt
     3721 gcgtgattaa caggaagtta tcatggctac agtaccaaca aggtcaggta gtccccgcca
     3781 gttaaccacc aaacagaccg gcgatgcgtg ggaagtacaa gcgcgtcgct ggctggaagg
     3841 caaaggactg cgatttgtcg ccgctaacgt gaacgagcgt ggcggtgaga tcgatctgat
     3901 aatgcgtgaa ggctggacca ccgtttttgt cgaggtgcgt taccgccgct ctgcgcttta
     3961 tggcggcgcg gcagccagtg tgacccgcag caaacaacac aaattattac agactgcccg
     4021 cttgtggctc gcgcgtcata atgggagttt tgatactgtg gattgccggt tcgatgtggt
     4081 agccttcacc gggaatgagg ttgagtggat taaggatgcc tttaatgacc actcataatt
     4141 aaggtttaag gattagcgtg caagaaagaa ttaaagcttg cttcactgaa agcattcaaa
     4201 ctcaaattgc ggcggcagag gcgcttccgg atgccatctc ccgtgcagcc atgacgctgg
     4261 ttcagtctct gctcaatggc aacaaaatcc tctgttgtgg taatggaact tccgctgcca
     4321 atgcacagca ttttgctgcc agcatgatca accgtttcga aacggagcgg cccagcttac
     4381 ctgccattgc actaaatact gataatgttg tcttaacggc gattgccaac gatcgcttac
     4441 atgatgaagt gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat gtattgttag
     4501 ccatttccac ccgtggcaac agccgcgata ttgttaaagc agttgaagcc gccgttacgc
     4561 gtgatatgac cattgtggca ttgaccggct atgacggcgg cgaacttgca ggtttgttag
     4621 ggccacagga tgtggagatc cgcattcctt cgcatcgtag tgctcgcatt caggaaatgc
     4681 atatgctgac ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc cctcaccagg
     4741 atgattaagg agaatacatg aaggcattat cgccaatcgc agtccttatt tccgcgctgc
     4801 tgttgcaagg ttgtgttgcc gctgccgtag tgggtaccgc tgctgtgggt accaaagccg
     4861 caactgaccc acgcagtgtc ggtacccagg tggacgatgg taccctggaa gtgcgcgtga
     4921 acagcgcatt gtcgaaagac gaacagatta agaaagaaac ccgtattaat gtcacggcgt
     4981 atcagggcaa agtgctgctg gttgggcagt caccaaatgc tgaactttcg gctcgcgcca
     5041 aacagattgc tatgggcgta gacggtgcca acgaagtgta taacgagatt cgtcagggcc
     5101 agccgattgg tctgggcgaa gcatctaacg atacgtggat caccaccaaa gtgcgttcgc
     5161 agctcttaac cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc gaaaacggtg
     5221 aagtgttcct gatggggctg gtgactgaac gtgaagcgaa agcggcggca gatattgcca
     5281 gccgggtgag cggcgtaaag cgggtaacca cagcgtttac gtttattaaa tagcagcccc
     5341 ttgtaatgcc tgatgcgacg cttgccgcgt cttatcaggc ctacaacgat gcaaaccgta
     5401 ggtcggataa ggcgtttacg ccgcatccga catgttttcc ctcaaatcag aacaacagcg
     5461 ccagcccggc caccagacaa atcgccgcaa aaccccagcc gaggacaaac cccataaaca
     5521 ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca ccgcccatcg
     5581 atacctgttg gcgacgcatc cccgccgcga ccggagccgc acagcaggta cacatcatgc
     5641 ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac
     5701 gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc acccccagca
     5761 ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc gcttgccacg
     5821 gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg gctgcagtaa
     5881 acgctttgcc gtagtaatgt tgccatttca cataccagag accggcaatg acaacgagaa
     5941 agaaaagcgc gggtttccac cactgaatgg gtgttgccgc ctgagatgaa gactgaccag
     6001 tcatagcatt ccctaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat
     6061 caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca
     6121 cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat
     6181 caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca
     6241 tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc
     6301 agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg
     6361 agtgggcatt ggcccccata gcactgacca ccagcatatg ctgcgcgccc agccgccgcc
     6421 cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc tctttgctcc
     6481 ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt tcggtgacct
     6541 gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc atatcgccca
     6601 acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc aacatccgca
     6661 gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc atcttcgctc
     6721 ctttacagaa ttgtctgcct tgcgctccac ggctcatgca ccagtcttaa tagaccatga
     6781 ggtaattatc cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca
     6841 tgagtaagaa aattgccgtt ttaatcagtg atgaatttga ggattcagaa tttacttcac
     6901 ccgcagacga gttccgtaaa gccggacacg aagtaattac cattgaaaag caagcgggta
     6961 aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc atcgatgaag
     7021 tgacgccagc ggagtttgat gccctgctgc taccgggcgg ccattcaccg gattatctgc
     7081 gtggggacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc aaacctgttt
     7141 tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac
     7201 tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgcggaa ttttacgatc
     7261 aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac gatctgccag
     7321 cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca aatcattttt
     7381 ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc
     7441 ggggccgaca gcattctggc aaccggaaag cgatgattgt acgccttgcg cgcgaggtcg
     7501 ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg aattaacgct
     7561 accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc actcatctgc
     7621 gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaacaga
     7681 taaaaagcat tagcgcacca cagttcccct tcctgctgca cacaccaggt gacaacatgt
     7741 tgttttgcca accaacggct gatggtgatg agtgtttcca ttgctgttct cttttatact
     7801 gtgggcctga ccttaacatg cgacgcagac gatgacaccc tggtttcttt acttgatccg
     7861 taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc gctatcagca
     7921 gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt
     7981 ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg ttaagcaact
     8041 gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag aactgttgag
     8101 cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc aaccagaccg
     8161 gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct ttcagtgccc
     8221 ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg actgtacagc
     8281 gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct
     8341 tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac cgccaacggt
     8401 gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag
     8461 ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca
     8521 cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg cagcagggca
     8581 tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg
     8641 tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca
     8701 gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca
     8761 catgcagccc cagctccgta tcgccttcaa tcaccagccg acgctggaag aagagcgtat
     8821 ctggatcttg tttacgcgcc gcaatcatca gcagatcgct gacgtcggca ctaaaactca
     8881 catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag gtaaaccatt
     8941 gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac tccagctcgc
     9001 catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta
     9061 gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac
     9121 gggaacgaag tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc
     9181 catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc
     9241 accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc
     9301 cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt atggagctgc
     9361 tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg
     9421 tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg
     9481 agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg
     9541 cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgcgcc gtggatatgg
     9601 cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gaatacgccg
     9661 ccgagcgtta tccgaatatt gagcgccacg tatcggtgca ggcttcggcg accaatgaag
     9721 aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt
     9781 tgtcgattca tcaggtgaaa cagctggcac gggtcacacc tgtaccactg gaagtgtttg
     9841 ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg
     9901 gtgagtcgcc taacaccgtg ggcgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa
     9961 cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat caggacggcg
    10021 aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc
    10081 acgcgctgga agaaccaacc agtctcaata ccttggaact gctgccggag ttaatggcgg
    10141 cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg
    10201 tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa agcgcgtgga
    10261 tggagacgct cgggtcgatg tccgaaggca cgcagaccac ccttggcgcg tatcaccgta
    10321 aatggcagtg agaagagcaa tgaaatattc cttagggcca gtgctgtggt actggccaaa
    10381 agagacgctg gaagattttt atcagcaggc cgccaccagc agcgccgacg tgatttatct
    10441 tggagaagcg gtatgcagca agcgccgggc aaccaaagtt ggcgactggc tggagatggc
    10501 aaaatcgctc gccggaagtg gcaagcagat tgtgctctcc acgctggcgc tggtgcaggc
    10561 atcctctgaa ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc
    10621 cagcgatctc ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca
    10681 cgcgctgaac tgctacaacg cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg
    10741 ctggtgtatg ccggtggagc tttcccgcga ctggctggtg aatctgctta atcagtgcga
    10801 tgagctgggc attcgcaacc agtttgaagt ggaagtcctg agctacggtc atctaccgct
    10861 ggcctactcc gctcgctgct ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga
    10921 aacctgctgc attaagtatc cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt
    10981 gtttgtactc aatggcattc agaccatgag cggctacgtt tacaacctcg gtaacgagct
    11041 gacatccatg cagggattgg ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt
    11101 cgcgatgctc gacgccttcc gcgctaatga aaatggcgct gcgccactat tgttgacggc
    11161 aaacagcgac tgtaacggct actggcggcg gctggcagga ctggagctgc aagcctaagt
    11221 aaatatgctc gtcatacttc aagttgcatg tgctgcatct gcattcgttc accccagtca
    11281 cttacttatg taagctcctg gggattcact ctcttgtcgc cttcctgcaa ctcgaattat
    11341 ttagagcaca gctcactttg ttaacaactt taactactct ttaatgcagt attaaagatt
    11401 aatcggtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg ttactcgatc
    11461 tggcccccat tcccgaaggt tcttcagcgc gagaagcatt ctcccactct ctcgatctcg
    11521 cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcagaacac cacaatatga
    11581 ctggcattgc cagtgctgcc acgtcggtat tgattggcta tctggcggcg aataccacca
    11641 cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg
    11701 aacagttcgg cacgctcaat acactctatc cggggcgaat cgatttgggg ctgggtcgtg
    11761 cgccaggtag tgaccaacgg acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg
    11821 ataatttccc ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc gatcccaatc
    11881 cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca
    11941 gcctttacag cgcgcaactg gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact
    12001 tcgcgccgga tatgttgttc caggcgctgc atctttatcg cagcaacttc aaaccgtcgg
    12061 cacgactgga aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc
    12121 gcgatgccga attcctgttt acctcaatgc agcaagcctt tgtgaagctg cgccgcggcg
    12181 aaaccgggca actgccgcca ccgattcaaa atatggatca gttctggtcg ccgtccgaac
    12241 agtatggtgt gcagcaggcg ctgagtatgt cgttggtagg tgataaagcg aaagtgcgtc
    12301 atggcttgca gtcgatcctg cgcgaaaccg acgccgatga gattatggtc aacgggcaga
    12361 ttttcgacca ccaggcgcgg ctgcattcgt ttgagctggc gatggatgtt aaggaagagt
    12421 tgttgggata gtgtgtctta acgcgggaag ccttatccga gctggcaacg ctgtcctacg
    12481 tagacctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc
    12541 accgctgatt actgatacac cggcagtaaa ttaaagctcg ataaaatatg taccaatgcg
    12601 ttgccgacgc caaacaccag aatcagcgta atcatcggct tgccgcccca gacgcggaat
    12661 ttcgggctgc caaagcgttt acgcgatgcg cgggccagca gtgccggaac aattgccgcc
    12721 cagatggtag ccgctaaacc agcataacca atggcgtaca ggaatccatt cgggaacagc
    12781 agaccaccca caacaggcgg tgcaaaggtc aacaatgctg ttttcaagcg gcccatagcc
    12841 gagtcatcga aaccaaacag atctgccaga tagtcaaaca aacccagcgt tacgccaagg
    12901 aacgaactcg ctaccgcaaa gtttgagaac acgaccagca gaaggtccag gctacggctg
    12961 ttcagcacgc cgcttaatgc ctgtaccagc acatcaatat taccgccctt ctctgcaata
    13021 ccgataaact ccggacgcgg gatgttaccc atcgtcgcca gcaaccagat ggtatacagc
    13081 gccagtgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg atctttgccg
    13141 taatacttca tcaggcttgg cacgttaccg tgataaccaa acgacgccag gcagaacggc
    13201 aatgtcatca gcagatacgg cgcataagat gcattgcttt ctgcaacgtt gaacaatgtc
    13261 gcaggctgca catgccccaa caggctgcca aaggtgagga agaaggtaat gactttcgcc
    13321 cccagcacaa tcgccgtcat gcggctgacg gccttagtac tcaaccacac cacaaacgct
    13381 accagcagtg caaaaccaaa acccgccgcc cgtgccggga cgtttagcga catctctgcg
    13441 aaggtgtgat gcagaattga accactggca gaaatatagg cataggtcag gatatagagc
    13501 acaaaggcaa tggaaatacc gttgaccacg ttccagcctt tgcccagcaa atctttggtg
    13561 atggtgtcaa aactcgaacc gattcggtaa ttcaggttag cttccagaat catcaagccg
    13621 gaatgcagca tacagaacca ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc
    13681 ccggacatga ccactggcag agaaaacatc cctgcgccaa taatggtgcc gccgataatc
    13741 accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc catgagggct
    13801 tctctccagt gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac cagtacgcga
    13861 gtacaaaaga cagaaaaaaa gccccgatgg taaaaatcgg ggctgtatat attattttac
    13921 agattgtgtt cgctgttcag cgatgattac gcatcaccac cgaaacgacg acgaccggta
    13981 gagtcatcac gacgcggagc gcggccttca cgacgttcgc cgctaaaacg acgaccatca
    14041 ccacggccac cttcacggcg ttcaccgctg aagttacgac cgccttcacg acgttcgcca
    14101 ccgaaaccac gaccaccgcc acgacgctca ccgccagtat gcggctgtgc atcgcccagt
    14161 aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg cagcacttca
    14221 cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt gatgttacca
    14281 atgtaacggc tgctgatgtc gccttcgtta gcaatcgcac caacgatatg acgaacttca
    14341 acaccatcat cacggcccac ttcaatgcgg tacagctgca tatcgccaac atcacgacgt
    14401 tcacgacgcg gacgatcttc acggtcacca cgcgggccac ggtcgttacg atcgcgcgga
    14461 ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc tggcggtacg
    14521 atcagagtac gttcaccctg tgccattttc agcagtgccg cagccagagt ttcgagatcc
    14581 agctcttcac cttctgcagt cggctgaatt ttgctcagca gtgcgcggta ttgatccaga
    14641 tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg gcgtttgccc
    14701 agcagttctg cgttcggcag ttctacttcc ggaatagtca gcttcatagt acgttcaatg
    14761 ttgcgcagca gacgacgctc gcggttctca acgaacagca gcgcgcggcc agcacgaccc
    14821 gcacgaccgg tacgaccgat acggtgaacg taagactcag aatccatcgg gatatcgtag
    14881 ttaactacca ggctgatacg ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc
    14941 aggatgtcca gacgaccatc tttcaggcgt tccagtgtct gttcacgcag cgcctggttc
    15001 atgtcaccgt tcagcgcggc gctgttgtag ccgttacgct caagagcttc agccacttcc
    15061 agagtcgcgt ttttggtacg aacgaagata atcgccgcat caaaatcttc cgcttccagg
    15121 aaacgtacca gtgcttcgtt tttgcgcata ccccagacag tccagtagct ctggctgatg
    15181 tcaggacggg tagtcacgct ggactgaatg cgcacttcct gcggctcttt cataaagcgg
    15241 cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt ctgatgacct
    15301 tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg cagcatttcg
    15361 tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc acgtttcagg
    15421 tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg cagggcgcgt
    15481 aattgcacgt cataacgctg gccgccgtac agagcaacca catttacgcc gcgcatgtgt
    15541 ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc
    15601 accagaatct gtggtgcttt cagctcagga tcaagattct gcaacagagg taaagagaat
    15661 gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc attcagcaga
    15721 tgtggaatac actctgcctg aattggagat ggtttttcgt aacccagatc gttaagggct
    15781 tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc agccatgtag
    15841 tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa attgatcagc
    15901 aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac aacaacgccc
    15961 tcacccctta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt
    16021 gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat tccaacaatg
    16081 cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg aacagtgccg
    16141 tggcgctgtc cgaatccccc agacttaggt agtacttacc taaatagaag ttggtttcac
    16201 tgagatgctc agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt tccattaacg
    16261 tttgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac tgttccttat
    16321 ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg agcttctgct
    16381 cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga taaaacgcca
    16441 gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcgatc ccgcgattca
    16501 agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct tcataggcag
    16561 catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaacact tcaggcatat
    16621 ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc agaccgagac
    16681 tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc
    16741 gactggcaag gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca
    16801 atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct gcaagcgtaa
    16861 gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac
    16921 ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt taaaagcccc
    16981 ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc ggagcttccg
    17041 gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg atacggccct
    17101 ggcgatcaac ttccagaact ttcaccggta cttcctgacc catctgcagg taatcggtca
    17161 ctttctcaac gcgtttgtca gcgatttgag agatgtggac cagaccttct ttaccgccgc
    17221 cgatggcaac aaatgcgcca aagtcaacga tacgggtcac tttaccagtg tagacgcggc
    17281 ccacttcgat ttctgcagtg atctcttcga tacgacgaat agcatgtttc gctttctcgc
    17341 cgtcggtcgc tgcgatcttc acagtaccgt catcttcgat ttcgatggta gtgccagttt
    17401 cttcggtcag ggcacggata acagagccgc ctttaccgat aacgtctttg atcttgtccg
    17461 ggttgatctt gatggtatgg atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt
    17521 tgatcgcctg ttccattacg cccaggatat gcagacgcgc acctttagcc tggttcagcg
    17581 caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc aatgcagaga
    17641 taccctcgcg ggaacccgca actttgaagt ccatatcgcc caggtgatct tcgtcgccca
    17701 aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt gcgatacccg
    17761 caacggcagc tttgatcggc acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg
    17821 aagccataga agaggaaccg ttggattcag tgatttcaga cacaacacgt acggtgtacg
    17881 ggaatttgtc catatccggc atgactgcca gcacgctgcg cttcgccaga cgaccgtgac
    17941 caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacggag tacggaggga
    18001 agttgtagtg gaacaggaag gtatcggtac gttcgcccat cagttcatca agaacctgcg
    18061 cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg cgtttcacca cgggtgaaca
    18121 gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac atccagacca cggatcatat
    18181 ctttttcacg accgtcgata cgcggttcgc ctgccagtac gcggctacga acaacgtttt
    18241 tctcgatagc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa
    18301 gcagcgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc tcttgtttgt
    18361 cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag tgcagcaacg cgcgcgttca
    18421 gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg tttaccggct tctttcacca
    18481 gttcattgat gttctgaata acaacctgct gttgttcatg accgaacact actgcgccca
    18541 gcatctggtc ttcgctcagc agttcagctt cagattcaac catcagaaca gcggcttcag
    18601 taccggcaac aaccagatcc agtttgctct ctttcagctc gtcctgagtc gggttcagta
    18661 cgtactggtc attgatgtaa cctacgcgag cagcaccaat cggaccattg aacggaatac
    18721 cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg ttaacttgcg
    18781 ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct tccgggaaca
    18841 gcgggcgaat cgggcggtca atcagacgcg cgatcagggt ttcgccttcg cttggacgac
    18901 cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc tcctgatagt
    18961 taacggtcag tgggaagaag tcctgacctg gtttggcttt tttctggcca acaacggtaa
    19021 caaataccgc ggtgtcatcc atgctaacca taacagcggc agtagcctga cgagccatca
    19081 tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg atcggattaa
    19141 gcaatgtaat atcctttctc tttcttagac agtaccttac ggcactggtg ttaatacccg
    19201 atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt gggtaaagcc
    19261 tctcattagc cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa tacattagtt
    19321 tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggccccc ttttctgaaa
    19381 ctcggcagga attagcgacg cagacccagg cgctcgatga gctgggtgta acgtgctacg
    19441 tctttacgtt tcaggtagtc gagcagttta cgacgctgag aaaccatgcg cagcagacca
    19501 cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt
    19561 gcagtcagca gtgctacctg aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac
    19621 tcagaaacga ttttagctgt tgcttcagta cttagagaca ttttaaaact ccaaagtata
    19681 tagaatgaaa ggacgccgat ctctaattca gcgatcccag tgtacgttac gcaaagtgtt
    19741 aaacaattta cgcgacgtta agcggcagta ttctactcgt agcgacttgt tatcgcaaga
    19801 cggttaacat tacgccgggt attcaaccac caggcgacga ggcgcgacgc ggccttcatc
    19861 gtcaatttcg cccataccga taaacttgcc gttctcacct tctgtaacgc gaaccagtcc
    19921 ttccagcggc gcaccggatg tacgaactgg gttaccattt ttgaagtaaa cagaagacgt
    19981 taacggaaga ttcaccaccg ggtagtccga agctggactg tccattggca tcagtaatgg
    20041 atcaagtaac tccgcagccg gaatatcctg ctgttcggct tgttcgacca gttcacgcag
    20101 atgttccagg gtcaccatcc gttcaaccgg atatttactt accgccagac gacgcaggta
    20161 aataacatgc gcgccacaac cgagtttttc acccaggtca tcaatgatgg tgcggatata
    20221 agtgcctttt gagcagtgaa tttccagctc cagctcattg ccttcgtggc gaataaacag
    20281 caattcataa atggtaatcg ggcgcgcttc acgcggtact tcaatgccct gacgcgcata
    20341 ttcgtacagt tttttaccct gatatttcag tgctgaatac atcgaaggga tctgttcgat
    20401 atcgccacgg aaagtatcca gcgccgccgc cagttgctct gcgctaaagg ttaccggacg
    20461 ttcttcaacg atttgtccgt cggcatcaga agtatcggta cgctgtccaa gacgcgcaat
    20521 gacccgatag cgtttgtcgg agtccagcag atactgggaa aacttcgtcg cttccccgaa
    20581 gcaaattggc aacatgccag ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt
    20641 ggcgttataa atccgtttca ctttttgcag cgcatcattg ctggacatac cctgcggttt
    20701 atccagcaac aaaacgccgt taatgtcgcg accgcgacga cgaggacgac tcattagtcc
    20761 tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc
    20821 aggtttgaca tgcgcatccc ttcaaccaga gagttgtcgt agaagaaggt cagttccggc
    20881 acgatacgca ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga agcttcttgc
    20941 aacgctttga tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat
    21001 actttggcat acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc
    21061 aggcgaggat ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc
    21121 gctacgcgct gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg
    21181 actgttagcc cctttttaaa attaatttca ggtggaaggg ctgttcacgt tggcctgata
    21241 agacgcgcca gcgtcacatc aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg
    21301 catggaaccc taaaaacctt aagcgatggt acgttggatc tcgatgattt cgaatacttc
    21361 gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg ccgataccac attccatacc
    21421 gttacggact tcgttaacgt catctttgaa gcggcgcagg gactccagct cgccttcgta
    21481 gataaccacg ttgtcacgca gaacgcggat cgggttgtga cgtttaacca caccttcggt
    21541 aaccatacag cctgcgatgg caccaaattt cggcgatttg aacacgtcac gaacttccgc
    21601 cagaccgata atctgctgtt tcagttccgg agacagcata ccgctcatcg ccgctttcac
    21661 ttcgtcaatc aggttataga tgacggagta gtaacgcaga tctaggcttt ccgcttcaat
    21721 cactttacgt gcagaggcat cagcacgtac gttgaagcca accaggatgg cgttggacgc
    21781 cgcagccagg gtggcgtcgg tttcggtgat accacctacg ccagaaccga tgatcttcac
    21841 tttaacttcg tcagtagaca gtttcagcaa ggagtcggag atcgcttcca cagaaccctg
    21901 tacgtctgcc ttcaggacga tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa
    21961 catgttctcg agtttagatt tctgctgacg cgccagttta acttcgcgga atttaccctg
    22021 acgatacagt gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc
    22081 cgcagccggt acgccggaca ggccgaggat ttccaccgga atggacggac ccgcttccag
    22141 cacttcctga cccagttcgt tacgcatcgc acgaacacga ccgtattcga agccacacag
    22201 aacgatatcg cccttgtgca gagtaccttc acgtaccaga acggtagcaa ccggaccacg
    22261 acctttatcg aggaaggatt cgataaccgc accgctcgcc atacctttac gtaccgcttt
    22321 cagctccaga acttccgcct gcagcaggat agcgtccagc agttcatcga tgccggtacc
    22381 cgctttcgca gatacgtgta cgaactggct ttcaccgccc cactcttctg gcaggatgcc
    22441 atactgggag agttcgttct taacacgatc cggatcggct tctggtttat caatcttgtt
    22501 cactgcaacc accaccggta cctgcgccgc tttcgcgtgc tggattgctt cgatggtctg
    22561 cggcatcacg ccgtcgtcgg cagcaacaac caggactacg atgtccgttg cctgcgcacc
    22621 acgagcacgc attgaagtaa acgcggcgtg ccctggggtg tccaggaagg tgatcatgcc
    22681 gttttcagtt tcaacgtggt atgcaccaat gtgctgggta atgccgcccg cttcgccaga
    22741 ggccactttc gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt caacgtgacc
    22801 catgatggtc acaaccggcg cgcgcggttc agccgcagcc cccgtgtcac ggtcgctcat
    22861 taccgcctct tccagctcgt tttcacgacg caggataact ttgtggccca tctcttcagc
    22921 aaccagctgt gcggtttcct gatcgataac ctggttgatg gttgccattg cgcccagttt
    22981 catcatcgct ttgatgacct gagagccttt aaccgccatc ttgttcgcca gttcgccaac
    23041 ggtgatggtt tcgccgatca caacgtcacg gttaacggcc tgagctggct tctggaagcc
    23101 ttgctgcagg gaagaacctt tacgttttcc gcctttacct ccacgtactg ctgcgcgagc
    23161 ttcttcacga tcagcttttg attcagcgtg tttgttgcct ttcttcggac gcgctgcttt
    23221 cgcgttacga ccacggccac ggccgccttc gacttcacga tcgctttcgt cttctgcctg
    23281 gcgagcatgt tgagaagtag tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt
    23341 atcagtccat ttgttttctt ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc
    23401 ttcttcgagt ttacgacgcg cttcttcttc agctttacgc ttgagctctg cagcttcctg
    23461 ctcacggcgg gctttttcag cctgggcgtt tttagtcata tcgtcttgtt gattgctcac
    23521 tttgtctttt tccgcagctt cacgtttcgc ttgttcagca gcttcacgct tagcttgttc
    23581 tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg
    23641 acgggcttgc tcttccgctt cacgctgcgc ttgctcttcc gcttcacgct gcgcttgctc
    23701 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    23761 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    23821 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    23881 caaagtctgt ttctcttgtg cggacacaga gtcgtcagca gacttccgga tacctgcatc
    23941 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    24001 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    24061 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca ccccttcgat
    24121 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    24181 ggccagtttg aatgccaaat cacgatctat cccttcaagg ttcagcagat cgtcagccgg
    24241 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    24301 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    24361 catcggcaca taggccagtt cttccagcgt cgagaagcct tcttctacca gaacagtcgc
    24421 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    24481 ctgatgctta gcctgcaggt cgtcaacggt catcacgttg agttcccaac cgctcagctg
    24541 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccggcttc
    24601 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    24661 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    24721 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacacaggc
    24781 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    24841 atcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttctggca cttcaatacg
    24901 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    24961 ttccgggcga acggaataga gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    25021 cggcagcata tcttcgcgca ggatcacggc ttcagcattg ttgcccagct ccagagagat
    25081 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    25141 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    25201 cgtctgggta gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    25261 caggttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    25321 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    25381 gatctgtacg cggacgtcga tctcttgttc atattttttc tttgttgctg tcgccagcgc
    25441 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    25501 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    25561 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    25621 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    25681 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    25741 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    25801 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    25861 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgtgaccgcg
    25921 aataaattcg atgccaacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    25981 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    26041 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    26101 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    26161 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    26221 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    26281 ccaccccgcg cctgaaactc ggcaaattct actcgttttg ggtaaaagat gcaaatactg
    26341 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    26401 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    26461 tgaatacttt tgccttctcc tgctctccct taagcgcatt attttacaaa aaacacacta
    26521 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    26581 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    26641 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttctggcggt
    26701 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    26761 actgcaaacc taggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    26821 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    26881 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    26941 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    27001 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    27061 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    27121 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    27181 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    27241 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    27301 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    27361 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcaacgacgt agaaatgatg
    27421 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    27481 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    27541 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    27601 gcgcatggtc gtcagttggg ccgtctactg taccaggggc gttggtttga ctcccaggcg
    27661 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    27721 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    27781 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    27841 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    27901 tatgccaaaa ctggcctgct ctcctcctct gccacttcag gcgtgccgca ggtggagaat
    27961 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcagggggg ggttaccttg
    28021 cttatcctct gtaaactcct ttctgaacat cacataatcg aaaacatcat ggtttgcatc
    28081 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    28141 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    28201 gttgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    28261 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    28321 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    28381 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    28441 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    28501 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    28561 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    28621 catggcgcgc atggcgatac tggtaactgc agtaccattc tgccgaaaag cggattgcga
    28681 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    28741 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    28801 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    28861 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    28921 ctcccccaca atcaacacgt aggtatcaat acctgtatcc ctgactgata attgaaaata
    28981 cggcaccgtg tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata
    29041 gttgagattg aaaaacggcg tataggtagc aaatcgcgac gctagtatat atggactgaa
    29101 cgttttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    29161 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    29221 tattattact gcccaaaaaa gaagggataa aaaagcaaag gcacatagat aagggatata
    29281 catccccagc attttgacaa cttcatccgg atcactctgg agcacactaa tcgcaaatcc
    29341 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    29401 agtcagtaac acaaatggtg cggcaacaag cagacgaagc ggaagggatg atatacgttt
    29461 tacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg cgccggaaga
    29521 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    29581 aaaacttcta gcaaatttgt tgaatactgt catccagaaa tccaagttca caaccaatat
    29641 aaacaattat caacaatgtt caattttata tcagacatgt ttttcaactc tgatccatat
    29701 cagaccgaaa cactgttagt tttttctttt taaatgatgc ggccattgcg ttctcagtaa
    29761 ttaaataaca ttatcaatgc gtctctaaac attacgcaga acgtagccat cagtagttat
    29821 actgacagga gcaggatggt ttatcgagaa gtaaacatat ccgtattcgt accggaactg
    29881 gagttgacac aataaagtgc caattatgtc agcagaaggg aaaagtttat aactaaggcg
    29941 taccggcacc atcgtttcaa ggtaccagct acgagtaaag caactggacg agatacagat
    30001 acctgacaac cattcctcag accaggacca aaacgaaaaa agacgctttt cagcgtctct
    30061 tttctggagt ttggtaccga ggacgggact tgaacccgta agccctattg ggcactacca
    30121 cctcaaggta gcgtgtctac caattccacc acctcggcac ggatactact ttttagttcg
    30181 ggatatcgct ggtcggctta gccggagcag ctggttgagt ttgttcggtt ttcgccggtg
    30241 cactcagatt ttcccattcg ctacctttat tggttttgtt gctgttgatg ttacccagca
    30301 ccagactgat gatgaagaat aacgttgcca gcagcgccgt catgcgggtc atgaagttac
    30361 cagaaccact tgaaccaaac agcgtagcgg aagcgcctgc tccgaaggag gctcccatat
    30421 cagcgccttt accttgctgc agcatgatca gaccaacaag gccaattgcc acaataagga
    30481 aaactaccaa aagagcttcg tacataatca acctgttcct tgcggagttg ccgcgtacca
    30541 atgcttcaac caataaagcg ggagttttta atctttccca ctgaagcggg tgtgaatact
    30601 aaccaaagcg aatgaccttc gcaagggcaa ttttatcgca ttgtatcaac tgcggaaaaa
    30661 aacagcaaaa ccgatgtatt cgctgagaaa aaaggcgaca atcgccgcct ttttagccag
    30721 tcatttatcg ttttaaacgg cttttactgc atcggcgatg cggtgtgcaa attcagtcac
    30781 ctgcgcttcg tcttcgcctt ccaccatcac gcgaattaac ggttcggtgc cggatttacg
    30841 cagcaacacg cgtccacggt tgcccagcgc agcttcaacc tctgcggtca cggctttaac
    30901 tgactcatgc tcaagcggat cgccgctacc tgcggtgtaa cgcacgttaa ccagaatctg
    30961 cgggaacatt ttcatgccgc tgcaaaggtc gtgcaggctc atatggttac gtgccatcgc
    31021 cgccagcacc tgcaagccag caacgatgcc gtcaccggta gtggttttat ccagcaggat
    31081 cacatgaccg gaattctctg caccgatacg ccagcctttc tcctgcattt tttccagtac
    31141 gtagcggtca cccactttcg cgcgcgcaaa tggaatacct aactgtttca gcgccagctc
    31201 aagccccatg ttactcatca atgtacccac agcaccacca cgcagctggc cctgacgaag
    31261 accttcacgc gcgatgatat acatgatctg atcaccatcg actttattgc cttcatggtc
    31321 aaccataatc acgcgatcgc cgtcgccgtc gaaggcaata ccgagatccg ctttttcagc
    31381 cagcacacga gcctggagcg cgcgaacgtc ggtagccccc acttcggcat tgatgtttac
    31441 accgttcggc tcacaaccga tagcgataac gttcgccccc agttcacgca gcacgttcgg
    31501 cgcgatgtga taagtcgcac cgtttgcgca atccaccaca atcttcagtt cgctgaggct
    31561 aagttcgttc gggaacgtgg ctttgcaaaa ctcgatatag cgacccgcag catcaacgat
    31621 acggctggct ttacccagtt ctgccgaatc aacacagctg atctcttttt ccatttccgc
    31681 ttcgatggcc tcttctaccg catccggtag tttggtgccg tcgatagaga aaaatttaat
    31741 gccattatcg tagaacgggt tatgcgatgc agatatcaca attccggcct ctgcgcggaa
    31801 ggtacgcgtc agataagcca cggccggtgt tggcatcggg ccagtgaaga gtgcggaaag
    31861 gcccgctgcc gccagacccg cttccagtgc tgactccagc atatagccag aaatacgcgt
    31921 gtctttacca ataatgatct tacgggagcc gtggcgcgcc agcactttac ccgcggccca
    31981 acccagctta agcacaaaat caggtgtgat cggcgcatcc cctacacgac cacgaatccc
    32041 atcggtaccg aaatatttac gattactcat agcgtttgtt ttcctttgca gacagagtgg
    32101 cttccaccac ccgcatcgct tctacggttt ctttgacgtc atgaacacga ataatgtgcg
    32161 cgccttgcat tgcggcaatg accgcacagg ccagactacc gctcaggcgc tcggacggcc
    32221 ccacgttcag cagctgccca atcatcgatt ttcgtgacat acctaccaac agcggcaggt
    32281 tgaaatggtg aaattcagcc aggcgcgcca gtaatgaata gttatgggag agatttttac
    32341 cgaaaccgaa tccggggtcg agcaacaatt tctcttttgc gatacccgcc tgctcgcaac
    32401 gtgctatttg ctcaataaag tagcgattca cttctgcaaa gacatcgtca tacttcggag
    32461 cttcctgcat ggtttttgga tttccctgca tatgcatcag acaaaccggt aaaccggttt
    32521 ctgccgccgc ctccagagcg ccaggttcgg aaagggagcg gatatcgtta ataatgtgag
    32581 cgccaacttt cgctgactca cggatgactt ctggtttgga tgtatctacc gagagccaga
    32641 cttcgaagcg ttgagcaatt gcctcaacca caggaataac acgttgcaac tcttcttcaa
    32701 cgctaacttc cgccgcccct gggcgcgtgg actcgccacc aacgtcaatg atcgtcgcgc
    32761 cagcgttgat catcagattc gcatgtttca ccgcatctat cagcgagtta tgcgtgccac
    32821 catccgaaaa ggaatcaggc gtgacgttga ggatccccat tacgtgagga tggctaaggt
    32881 ccagtgaagt gccctgggca aagagtttca tggtgttatc cctggtatga attgataaga
    32941 aaaaaccccg gagcacgccc cggggttttc ggtacaaata cagtcatctg atgcgggaac
    33001 ttacttgtcg cctaactgct ctgacatggt gttacccggg ttcggcgtac gcggttcatc
    33061 aaccggacga ggagcctttg gactaccatt gtcgccagaa ttgttagaag cgcctggttc
    33121 ttcccagccc gctggcggac gtacatcgcg acgtgccatc aggtcatcaa tctgcggtgc
    33181 gtcgatagtc tcatatttca tgagagcatc tttcatcgca tgcagaatat ccatattgtc
    33241 ggtcagaagc tgacgcgcac gattatagtt acgctcaatc agtgctttca cttcctggtc
    33301 gatgatacgt gcagtttcat cggacatatg tttcgctttc gctacgctac ggccgaggaa
    33361 cacttcacct tcttcttccg cgtacagcag tggacccaat ttctcagaga agccccactg
    33421 agtcaccata ttacgcgcca ggttggtcgc aactttaata tcgttggacg caccggtaga
    33481 aacatgttcc ggcccgtaga tgatctcttc tgccagacga ccaccgtaca gcgtagaaat
    33541 ctggctttcc agtttctgac ggctggcgct gattgcgtcg ccctcaggca agaagaaagt
    33601 cacacccagc gcacgaccgc gtgggataat cgtcactttg tgcaccggat cgtgttccgg
    33661 caccaagcga ccgataatcg catgacccgc ttcgtggtaa gccgtcgatt ctttctgcgc
    33721 ttccgtcatc accatggagc gacgttccgc acccatcatg attttatctt tcgctttttc
    33781 gaactcaatc atcgacacaa cgcgtttgtt gccacgagca gcgaacagtg ccgcttcgtt
    33841 taccaggttc gccaggtcag caccggagaa accaggagta ccacgggcaa tgattgccgc
    33901 gtcgatatcg ggtgccaatg gtacgcgacg catgtgaact ttcaggatct gctcacgacc
    33961 gcgaacatct ggcaagccaa ccacaacctg acggtcgaaa cggccaggac gcagcagggc
    34021 tgggtcaaga acgtccggac ggttagtcgc ggcgataacg atgatacctt cgttaccttc
    34081 gaagccatcc atctcaacca gcatctggtt cagagtctgt tcacgttcat cgtgaccacc
    34141 gcccagacca gcgccacgct ggcggcctac ggcgtcgatt tcatcgataa agatgatgca
    34201 cggtgccgct ttcttcgcct gttcgaacat gtcacgaaca cgggatgcac ccacaccgac
    34261 gaacatttct acgaagtcag aaccggagat agtaaagaac ggaactttcg cttcgcctgc
    34321 aatcgctttc gccagcaacg ttttaccggt acccggagga ccgaccatca ggacgccttt
    34381 cgggatctta ccgccgagtt tctggaagcg gctcggctcg cgcagatact ctaccagttc
    34441 agcaacttct tcttttgctt cgtcgcagcc cgcaacgtca gcaaaggtcg ttttgatctg
    34501 atcttccgtc agcatgcgcg ctttgctctt accaaacgac atggcacctt tgccaccgcc
    34561 gccctgcatt tgacgcatga agaagatcca gacaccaatc agcaacagca tcgggaacca
    34621 agagatgaag atagaagcca gcaggcttgg ttcttcaggc ggttcaccga caaccttgac
    34681 gttcttggtc aacaggttat ccagtaattt cggatcctga accggaatgt aagtggtata
    34741 acggttacta tctttcttgg taacgttgat ttcacgtccg ttgatacgcg cttcacgaac
    34801 ctggtcgtta ttgacctctt gtaggaaggt agagtaatcc accttacggc cattagactc
    34861 gctgggccca aagctctgga atactgacat cagcacaacg gcaatgacca gccagagtat
    34921 taggtttttc gccatgtcac tcaagggatt aacctcttat tacaactgtg ttaaaaacag
    34981 cgtcaggata ctctatatcc agcatctttc aaactttcgt ctgaaatctc ccggttaggg
    35041 tttacgcccg gtcgctacaa tatacacttc ccgcgaacgt gcacgagaag agtccggctt
    35101 acgaactttg accttcgtaa acagggagcg aatttccctt agatactcat cgaaaccttc
    35161 gccctggaac accttcacta caaaactgcc acctggcgct aatacatcac gacacatttc
    35221 taacgccagt tccaccagat acatggcacg ggggatatcc accgccggtg ttccgctcat
    35281 gtttggtgcc atatcggaca tgacaacctg gactttgctg tcgccaacgc gctccagcag
    35341 tgctttcatc accagttcat cacgaaaatc gccctgaaga aagtccacac caacgatagg
    35401 atccataggt aaaagatcgc aagcgatgat gcggcctttg ccgccaattt gggtgaccac
    35461 atattgtgac caaccacccg gagcagcacc aaggtcgaca accgtcattc ccggtttaaa
    35521 gagtttgtca ctttgctgta tttcatcaag tttaaaccag gcacgggaac gtaacccctt
    35581 tttctgtgcc tgttgaacat atttatcgct aaagtgttcc tgaagccagc ggctggagct
    35641 ggcagaacgc ttcttacctg tcatttaact ttcccatggg gataactcat cgtaaccaat
    35701 tgcgtaaatt tttacgcgcc tatttggtga tatatgggag atggcggtag aatgacccgt
    35761 tttcaatccc aacgtaagca aaaaatacga tgaatctgag tactaaacaa aaacagcacc
    35821 tgaaaggtct ggcacatccg ctcaagccag ttgttctgct tggcagtaat ggtttgaccg
    35881 aaggggtgct ggccgagatt gaacaagcgt tagagcacca tgaactcatc aaggtgaaaa
    35941 tcgccaccga agatcgcgaa acaaaaacct tgatcgtgga agctatcgtg cgcgaaaccg
    36001 gcgcctgtaa tgtacaggtc atcggtaaaa cgctggtgct ttatcgccca actaaagaac
    36061 gtaaaatctc gctgccacgc taagattatc ctaaagttac acacatttgc tgtgtaaaac
    36121 gaggggtttt ccgcaggcag gagagcaaaa tgccacgctc tgttcgttga taaaaggccg
    36181 catagcggcc tttttccttt ctttacaata catcaacatc ttgatgtatt gggtaattct
    36241 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    36301 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    36361 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    36421 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    36481 ttggtgacat caatcacctg cgcgttcgac agcttggctt cgatgtcttt aatacggcct
    36541 tcgcagaaac cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    36601 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    36661 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    36721 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    36781 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    36841 ggtcagctac aattcacatt ttgatagtca ttttaccctg aagttcccga agggtcatcg
    36901 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    36961 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    37021 gccgcgaatg ttgatgagta cgttactcaa ctccccgctg gtgccaacct tgccctgatg
    37081 gtgcaaaaag tcggcgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    37141 cccgccagta cgcagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    37201 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    37261 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    37321 atcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    37381 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    37441 tttagcgctc cgcctgccgc tgccatagtt gaccgcaact gtttctccgt ctcgctctac
    37501 agtgccccaa aacctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    37561 atgttcagcc aggtacgcac cctcccccga ggttctgccg aagcgcaata ctgcgaactg
    37621 gatgtcatac caggcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    37681 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg ggcaattctg
    37741 aaagatgagt taaaacaggc aggtatcacc tggagcggaa cactgctgcg ccagactcag
    37801 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgttgca cgatctgctt
    37861 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgataggc
    37921 catgcgcgct tcaatgtgcc tggaacatgg cgggccgggt cggacgccgt gcgtcagatc
    37981 ctgcgccagc aagccggtgt cgatattgga aacaccatta ttgccgatgg ttcagggctt
    38041 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgttgcaata cattgcccaa
    38101 cacgacaatg aacttaactt tatctccatg ctgccgctgg cgggctatga tggctctttg
    38161 cagtaccgtg caggtctgca tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt
    38221 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    38281 gcgtttgtac aatatctttc tggctatgca gtagaaccgg cggatcagcg taatcgccgt
    38341 attccgttag tgcgttttga aagccgtttg tataaagata tttatcagaa caattagtca
    38401 aaaagaaacc ccggcacatg gccggggttt tgctgattaa cgcttgtaaa tgaactcaac
    38461 gccttcttcg tcgtcttcgt cccagtcatc atcccagtcc tcatcatcct cttcagcaat
    38521 ctcttcaagc tgctggcgat gataatcatc ccacatgaat tcgactttct ctggctgttt
    38581 cgcttcttca gcctgcacga ccgggttttc aatgataaag gtcatcacat cccagcagag
    38641 atctttcacg cccagtccac tcgccgcaga gatcagataa tatttatctt cccagcccag
    38701 cgcttcagca atcgctttcg ctttctcttc agcttctgcc ttatccagca gatcgatctt
    38761 attgaacact aaccaacgcg gtttcgccgc cagatcctgg ctgtattttt ccagctcgct
    38821 gataataata cgcgcgtttt caaccggatc ggtgccgtca atcggatcga tatcgatgag
    38881 gtgcaacagg acgcggcaac gttccaggtg cttcaggaag cggataccca gacctgcgcc
    38941 ttccgcagcg ccttcaatca gtcctggaat atcggcaaca acgaagctct tttcgttgtc
    39001 cattcgtacc acacccagac ttggcaccag agtggtaaac ggataatccg ccactttcgg
    39061 tttagccgcc gataccgcgc gaataaaggt cgatttaccc gcgtttggca tccccaacat
    39121 accgacgtca gccagcagca tcagctccag cagcagctca cgcttatcac ccggcgtacc
    39181 gttggttttc tgccgtggtg tacggttaac ggacgatttg aaacgggtat tgcccagacc
    39241 gtgccagccg cccttagcaa ccagcagacg ctgaccgtgt ttagtcatat cgcccatggt
    39301 ttcaccagta ccctggtcga ttacacgcgt acctaccggc actttaatcg tcacgtcttt
    39361 accgcgctta ccggtacaat cgcggcttgc gccgttctga ccgcgctctg cacggaaaga
    39421 tttttcaaaa cgataatcga taagcgtgtt caggttttcg tcggcttcca tccatacgtc
    39481 accaccatca ccaccgtcgc cgccatccgg gccgcctttc ggaatatact tttcgcggcg
    39541 gaagctcacg caaccattac cgccatcacc tgcaacgacc agaatcgatg cttcatcaac
    39601 aaacttcatt ttattctccg taaatcattc gcctgcgcgg ggttgcgata ccaccgttgt
    39661 atgcttacgt aatccgcccc aaatacgatg accaatggcg gaatacatcg cgcccgcaac
    39721 cacgacaaac gcaccgagat aacctaaaag gtttaacatc ggtctggcga agaaatcggg
    39781 ccaggccagt gataaaagat ctgaaaaaaa cagcgtaaac agtggggtga gcgtgatgat
    39841 cgcgctcacc tgcgctgcct gccagcgagc catcgcttcc gccagggcgc catatcctac
    39901 caatgtattc agtccgcaaa aaattaaaca tgcgagctgc cagtggctaa gctgcgctat
    39961 cactcctggc tttgccagag ggaagagcgc aattgtacat aaagtgtaca gcaaaaacag
    40021 gatctgcggt gaggccagcc gacgcaataa aaccttttgc gccacgccat aactcaccca
    40081 aaccgtcgcc gcaccgaccc caaagatgac tccccaggtg taatcagtga gctttgtaaa
    40141 tatctcgacc agactggtgt taaaaaacat caccaggccg ctcaggagca tcaatgcccc
    40201 tacaacctga gtgctgcgca ttttctcttt caggataaat acgctggcaa ccatcatgcc
    40261 aactggcgag agttgcccaa tcacctgcga agcggtcgga ctcaggtatt gcaaggatga
    40321 gctgaacagg atgaagttcc caaacagccc ggcggtcgcc actgccaaca aaatcaacca
    40381 gcgtggctta cgaaacacgc gtaatggcgg caacctcttc ttcaccgcaa gaatggcacc
    40441 caggccaata ctcgccatca agaaacggta aaacacgatt gtcggaggtt ccatcacctc
    40501 cagcacctgc ttcattgcga ttggcaacgc cccccagcaa attgctgtgg tgagcgccaa
    40561 aagaatgcca atgcctgcct gctgcttcat accgtattcc ctgcaaagcg catttgcgga
    40621 ttaacgtcgc accatgctca tttttaccgg ttatcgaatg taaaaagccc cgcaacgtgt
    40681 tgcggggctt tcatccgtta ccgggacgcg aaaaacttat tcagcttcga tgctgataaa
    40741 tttacggttt ttcgggcctt taacttcgaa tttcacttta ccgtctgctt tagcaaacag
    40801 agtgtggtca cgaccgcaac ctacgttagc gccagcgtgg aatttggtac cacgttgacg
    40861 aacgatgatg ctacccgcca gaacggattc gccaccgaaa cgcttaacgc ccaggcgttt
    40921 agcttctgaa tcgcgaccgt tacgtgtgga gccgccagcc tttttatgtg ccatttgaaa
    40981 tctctcctca ggtcttaggc gctgatgcca gtaattttca catcagtgaa ccactgacga
    41041 tggccctgct gcttacgata gtgtttacga cgacgaaact taacgatttt aactttctcg
    41101 ccacgaccgt gagcaacaac ttcagctttg attacgccgc catcaacgaa aggaacgccg
    41161 attttgactt cttcaccgtt tgcgatcatc agcacttcag cgaactcaac agtttcgcca
    41221 gttgcgatgt ccagcttttc caggcgaacg gtctgacctt cgcttactcg gtgttgttta
    41281 ccaccacttt ggaaaaccgc gtacatataa aactccgctt ccgcgcacac cttttcaatg
    41341 attcagagtg cgctataaat attcacaata gggcgcgaat attacgcaaa acgcacgcct
    41401 ttgacaagtg ctacagtcaa tacacgaaga aaaaaaacac aacttgtacg gtaacgttta
    41461 tctgtgccat tttttcagta caatcaccct atattcctaa ccataaaccc taagttgcct
    41521 ttgttcacag taaggtaatc ggggcgaaaa gcccggcttt tgcgatgaat ttagaaaaaa
    41581 tcaatgagtt aaccgcgcaa gatatggcgg gtgttaatgc ggcaatcctt gagcagctta
    41641 attccgacgt ccaactgatc aatcagttag gctattacat cgtcagcggc ggcggtaaac
    41701 gtattcgtcc gatgattgct gtactggctg cacgagctgt tggctatgag ggaaatgcgc
    41761 atgtcaccat tgctgccctg atcgagttta tccacacggc gactctgcta cacgacgacg
    41821 ttgtggatga atcagatatg cgcaggggta aagctaccgc caacgctgca tttggcaatg
    41881 ccgccagcgt gctggtaggc gattttattt atacccgcgc gttccagatg atgaccagcc
    41941 tcggttcact caaagtgctg gaagtcatgt cagaagctgt aaacgtcatc gcagaaggtg
    42001 aagttctgca actgatgaac gttaacgatc cggacatcac tgaagaaaac tacatgcgcg
    42061 ttatctatag caaaaccgcg cgtctgtttg aggctgccgc gcagtgttcc gggattctgg
    42121 ctggctgtac gccggaggag gagaaaggcc tgcaggatta tgggcgctat ctcggcactg
    42181 ctttccagtt gatcgacgat ttactcgatt acaatgccga tggcgaacag ttaggtaaaa
    42241 acgtcggcga cgatctgaac gaaggtaaac cgacgctgcc gctgctgcat gcgatgcatc
    42301 atggcacacc agaacaggca cagatgatcc gtaccgccat cgaacagggt aacggtcgcc
    42361 atcttctgga accggttctg gaagcaatga acgcttgtgg atctcttgaa tggacgcgtc
    42421 agcgcgccga ggaagaagca gacaaagcca tcgcagcgtt acaggtgctc ccggacaccc
    42481 cttggcgaga agcactcatc ggcctcgcgc acatcgctgt tcaacgcgat cgttaatccc
    42541 ctcccttcat cccgcgcagt gcgcgggatg attccagtaa attccataaa aacactaatt
    42601 cagcgctata acacctgaaa aaaagggcga tgaatcttta tatattctga atattcacac
    42661 tctttacagg aactttttag agcaataggc catcaggagt atagtgatgc ccgacagaag
    42721 aagtgttctg aatgaaagcg actaacttaa ggagtgagga aagatggaaa gtaatttcat
    42781 tgactggcat ccccctgaca tcattgcagg tttgcgcaag aaaggaacgt caatggcggc
    42841 ggaatctcgc agaaatggtt tgagttcctc aacgctggca aacgcattat cgcgcccatg
    42901 gccgaaagga gagatgatta ttgcgaaagc cctgggaact gacccctggg ttatctggcc
    42961 atcacgctac catgatccgc agacccatga gtttatcgac agaacgcagt tgatgcgtag
    43021 ctacactaaa ccgaaaaaat gagtggtctg gcggtagccc cgcgaacggg gctgccagct
    43081 ctcagacgat tactcgcctt tcacacgctt aatatttgca cctaaagcgc gcagtttgtc
    43141 ttcaatgcgt tcgtagccac gatcgatgtg ataaatacga tcaaccaccg tcgtcccttc
    43201 cgcaatacag ccagccagca ccaggcttgc tgatgcacgc agatcggttg ccataacctg
    43261 tgcaccagaa agtttttcaa caccatgaca aataacggta ttgctttcga tttcggcatg
    43321 cgcgcccata cggctaagct ccggcacatg cataaagcgg ttttcaaaga ccgtttcggt
    43381 gatgaatccg gtcccttctg ccaccaggtt caacagcgtg aactgggcct gcatatcggt
    43441 cgggaatgcc ggatgcggcg cggtacgtac gttaacagcc ttcggacgtt tgccatgcat
    43501 atccaggcta atccagtctt cgccgacttc gatgtccgct ccagcgtcac gcagtttcgc
    43561 cagcacggcg tcgagagtat ctggctgcgc gttacggcag ataattttgc cgcgagaaat
    43621 cgccgccgcc accaggaaag taccggtttc gatacgatcc ggtagcacgc gatagacacc
    43681 gccgcctaaa cgttccacac cttcgatgac gatacgatcg gtgccctgac cgctaatttt
    43741 cgcacccagc gtaatcagga agttcgcggt atcgacgatt tccggttcac gcgctgcgtt
    43801 ttcaataatc gtggtacctt ccgccagggt tgcagcacac atgatggtca ccgttgcgcc
    43861 aacgctgact ttatccatca cgatatgtgc gcctttcaga cgaccatcga cggaagcttt
    43921 aacgtaacct tcttccagtt tgatggtcgc gcctaattgt tcgaggccag aaatgtgtag
    43981 atcaaccgga cgcgcaccga tcgtacaacc gccaggcagt gaaacttgcc cctgaccaaa
    44041 gcgcgctacc agcggcccca gtgcccagat agaagcacgc atggttttaa ccagatcgta
    44101 aggtgcgcag aatacattaa cgtcgcgggc atcaatatgc acagaaccat tacgttctac
    44161 tttcgcaccc agctgactta gcagcttcat tgatgtatcg acgtctttca gtttcgggac
    44221 gttctggatc tctaccggtt cttccgccag tagagcggca aaaaggatag gcagagcagc
    44281 atttttagcg ccggaaattg tgacttcgcc ctggagcttc gttggcccct gaacacgaaa
    44341 tttatccatt tagtttgttc tcagttaaca attcatatcc gctaccggcg aatcgcccat
    44401 agctcaaaag ccgttcagtt tgcgatcgcg cgcccactcc gcaggggtat acgctttgat
    44461 cgacacagca tgaatgcggt tatccgcaat atattccatc agcgggccat agaccgtctg
    44521 ctgtttttta acccgactca tgccgtcaaa caactcaccc acggcaataa cctgaaagtg
    44581 gctgccatcg ccggaaacgt ggacttcctg gagggagaga gcgttcatca acacgctctg
    44641 aatttcatta ttttccatgg gatcttcaat catcagttaa taaaccagcg aaacatctta
    44701 gagcaaagtt gcgctggcat aaataagcaa aaagcctcgc tgataaatca gacaaggctc
    44761 gacttgcagg caggtttgcc ggacaggcgg ttaacgccat atccggcctg aaaaaattta
    44821 acgaggcaga acatcagcag gcaaattata caatttcgcc agggtataca ctttgtcgtt
    44881 taccccctga agcgtcacat tgttgccctg ctttttcgcc agatcgataa gatggagcag
    44941 cagtgccagt ccccccgtat ccacgcggga cacacggcta agatcgatgc aggtaatccc
    45001 cttcaccgct tcctcacgca tttcccaaag cggtagcaaa acgtcctgat ccagctctcc
    45061 ggataacgcc agcgtgtcac ccgtctgcat ccagctcagt gactcgctca ttattttttc
    45121 tcttccagag tgattttctg ttgagaaatc gatttcagtt gcgcagtcag gccgtcgata
    45181 cctttggtac gcagcagcgt tccccactcg ttttgtttgg tggtgatcat actgacgcct
    45241 tcagcaatca tgtcgtaagc ctgccagttg cccgtctggg agtttttacg ccactggaag
    45301 tccagacgca ccggcgggcg gccattcgga tcaataatgg taacgcgaat cggcacaatg
    45361 gttttatcgc ccagcggctg ttctggcgca atctgatagg tttgaccatg atacatcgcc
    45421 agcgcctgac cgtaagcctg cttcaggtac tcacggaaag cggcaaagta ggcttcacgt
    45481 tgagcagggg tcgcactctt gtaatactgg cccagcacca gcgcaccggc gtatttcacc
    45541 tgtacgtatg gcagcagttc ctgatcaaca atggtacgca gataatccgg gttggcccga
    45601 atttgcggtt gctcattctt caggcgatcg aacgttttct gcgccgcctc gtccatcagc
    45661 ttataaggat tggtctggtc tgccgcggtt gccgcactca gcggtgcaat caccagcaaa
    45721 gcgaccatca ttaaacgttt aaacatgcgt cggttctcct gaaattattt cgttgtaccc
    45781 acaggttcag tggtttcatt attacctggc gcagcagctg gcgcatcgcc actattctta
    45841 ttgtcatcgc ctttactacc gtaaaggaac tgaccaatga gatcttccag caccatcgca
    45901 gacttagtgt cctgaattgt atcgccatcc ttcaggatag cagtccccag ttccgggtct
    45961 tcaaaaccga cgtttaatgc cagatattgt tcccccagta ggccggaagt acgaatgctc
    46021 agcgaactgg tatctggaat gtggttataa cgttgttcaa tttccagcgt tacgcgtggc
    46081 agataggttt tcgggtccag cgtaatatcc gccacccggc ccacaacaac gccaccaata
    46141 ctgaccggag agcgcgcttt caggccgcca atgttatcga acgtcgcata aagcgtgtag
    46201 gtcggttcag tacgtatgga cgtcacgttc gccgccttca ggcaaacaaa cagagccgcc
    46261 agcagtgctg ctaataaaaa gatacccacc caaatttcat tttttttcgt ttgcatgtac
    46321 tcaattccca aacatcaatg cggtcagcac aaaatccagc cccagaacag ccagagacga
    46381 gtggacaacg gtgcgagtgg ttgcccggct aatcccggca gacgtcggga tggcgtcgta
    46441 gccgttaaac aacgaaatcc acgtcaccgt gatggcgaac accacgctct taatcagaca
    46501 gttgaccaga tccatacgcc agtcgacggc attttgcatt gccgaccaga agaacccgct
    46561 atcaatgcct ttccaactga cgccgaccag agatccgccc cagatcccca cggcgacgaa
    46621 aataaccgtc aacagtggta atgaaataac cccagcccag aaacggggag aaataacccg
    46681 acgcagcgga tccaccgcca tcatctccat actggagagt tgctctgtag cgcgcatcag
    46741 gccgatttct gcggttagcg ccgaaccagc acgcccggca aacaacaacg cggcaaccac
    46801 cggccccagt tcacgcagta gcgataacgc caccagcata cccagactgg tttccgcact
    46861 ataagtggtc agaaccagat aaccttgcag ccccaacacc attccgatga acacgccaga
    46921 aaccacaata atcagcatcg acaggacgcc gacattatag agctggcgca ccagcagcgg
    46981 cgcatgtttg cgaaattccg gtttgccgac cagcgcattg aataacatta acccggcccg
    47041 cccgaacgtt ctcagggttt taatcccttt atgtccgagc gacgccagcg catttaacag
    47101 catgagtggc ttaactccct ggtaaaagat cagcgtgata atcgccggca ggatagcgga
    47161 acggaacagg cccgtcagct atcccgtcca gaaactgacg tacgcgcgga tcaggattcg
    47221 cctgcaacgc ctgggcacag ccatgagcga caattttttt gtccgccagg atccaggcgt
    47281 gatccgcaat acttaacact tccggcacat cgtgagaaac caccacacaa gtcacgccca
    47341 gcgcgctgtt cagctcagaa atcagcttca ccagtacgcc catggtgatg ggatcttgcc
    47401 caacaaaagg ttcatcaaac atgatgagat ccggctccag cgcaatcgca cgcgccagcg
    47461 ctgcacgccg cgccatccca ccggaaagtt cagaaggcat tagtttagcc gctccacgca
    47521 gccccacggc ctccagcttc atcatcaccg tactatgcaa caatggcgcg ggaagttggg
    47581 tatgttcgcg cagtggatag gcgacgttgt caaatacgtt catatcagtg aacaacgccc
    47641 cggactgaaa taacatgctc atccgtttgc gcactgtata caggcgcgaa cgagacatcg
    47701 ccggaatatt ctcaccatcg aaaaggatct caccatgatc tggtgcgatt tgcccgccaa
    47761 tcagacggag tagcgtcgtt ttaccgatgc ccgatggccc catgatcgcc gtgatcttcc
    47821 ctcgcggcac ggtcagggaa atattatcga agatgcagcg attgccacgc gtaaaactga
    47881 catcgcgcat atcgactaaa ttcgccacag actgctccat aattcaccct tcgtcttgcg
    47941 ttgattttct aagcatggcg ctcaatttaa ccttgaaccc aacatattta cagaatatta
    48001 cccgctgtgg ttagcgaaag ctggcatttg ttttactttt tagccgcata aagtcaaaat
    48061 taagaatccg ttacggcttt ctgaaaatct tcagcggacc ggcgagtata cctgaagaaa
    48121 ggacgttaga tgcttttagc tacggcactg ttaattgttg gtttactttt ggtcgtttac
    48181 agtgccgacc gcctggtttt tgccgcgtct attctttgcc gaacctttgg cataccgccg
    48241 ctgatcatcg gcatgacggt ggtcagtatt ggtacatcgt taccagaaat catcgtctcg
    48301 cttgccgcgt ctctgcacga acaacgcgat ttagccgttg gtacagccct cggctcaaac
    48361 attatcaata ttttgctgat cctcggtctg gctgcgctgg ttcgtccttt taccgtccat
    48421 tccgatgttc tgcgtcgtga attaccctta atgttgttgg tcagcgtagt ggccggttcc
    48481 gtactctatg acggacaact tagtcgcagc gatggtatct ttctcctctt tctggctgtg
    48541 ctatggctgc tgttcattgt taaacttgca cgtcaggctg aacgtcaggg gactgacagc
    48601 ctgaccagag agcagcttgc agagctgccg cgtgacggcg gattgcccgt cgcgttttta
    48661 tggctcggca ttgcgcttat catcatgcca gtggccacgc ggatggtggt tgataacgcc
    48721 acggtgctgg cgaattactt tgccatcagc gagttgacga tgggtctgac ggcaattgct
    48781 atcggaacca gcctgccgga actggcaacc gcaatagcgg gggttcgcaa aggtgaaaac
    48841 gacattgctg tcggaaatat cattggcgca aacattttta atattgtcat cgtgttgggt
    48901 ttacccgcgc tgataacacc aggagagatt gatccattgg cgtacagtcg tgactacagc
    48961 gtgatgttgc tggtgagcat tatttttgcg ttgctgtgct ggcggcgctc cccgcaaccg
    49021 ggccgtggtg taggggtatt attaactggc ggatttatcg tatggctggc gatgttgtac
    49081 tggttatcgc caatactcgt tgaataactg gaaacgcatt atgtcgcacg tagagttaca
    49141 accgggtttt gactttcagc aagcaggtaa agaagtcctg gcgattgaac gtgaatgcct
    49201 ggcggagctt gatcaataca tcaatcagaa tttcacgctt gcctgtgaaa agatgttctg
    49261 gtgtaaaggg aaagttgtcg tcatggggat ggggaaatcg gggcacatcg ggcgcaaaat
    49321 ggccgcaacg tttgccagca ccggtacacc ttcatttttc gtccatcctg gtgaagccgc
    49381 gcatggtgat ttaggcatgg tcaccccaca ggatgtggtg attgctatct ctaactcagg
    49441 tgaatccagc gaaatcacgg ccttaattcc agtgcttaag cgtcttcacg taccgttaat
    49501 ctgcatcacc ggtcgcccgg agagcagcat ggcgcgcgcc gcagatgtgc atctgtgtgt
    49561 taaagtagcg aaagaagcct gtccgttagg gctggcaccg accagcagca ccaccgccac
    49621 gctggttatg ggcgatgccc tcgctgtcgc gctgttaaaa gcacgcggct ttactgctga
    49681 agattttgcg ctctcacacc caggcggcgc actgggtcgt aaacttctgc tgcgcgtaaa
    49741 cgatattatg catacgggcg atgagatccc gcatgttaag aaaacggcca gtctgcgtga
    49801 cgcattgctg gaagttaccc gcaaaaatct tggtatgact gtcatttgcg atgacaatat
    49861 gatgattgaa ggcatcttta ccgacggtga tttacgccgt gtcttcgata tgggagtgga
    49921 tgttcgtcag ttaagtattg ccgatgtgat gacgccgggg ggaatacgcg tgcgccctgg
    49981 cattctggcc gttgaggcac tgaacttaat gcagtcccgc catatcacct ccgtgatggt
    50041 tgccgatggc gaccatttac tcggtgtgtt acatatgcat gatttactgc gtgcaggcgt
    50101 agtgtaaaga ttcaaggata aacaacaatg agcaaagcag gtgcgtcgct tgcgacctgt
    50161 tacggccctg tcagcgccga cgttatagca aaagcagaga acattcgtct gctgatcctc
    50221 gacgttgatg gcgtactgtc agatggcctg atttatatgg gcaataacgg cgaagagctg
    50281 aaagcgttca atgttcgtga cggttatggc attcgttgtg cgctcacctc tgatattgaa
    50341 gtcgctatca ttaccgggcg aaaggctaaa ctggtagaag atcgttgtgc cacattgggg
    50401 atcactcact tgtatcaggg gcagtcaaac aaactgatcg cctttagcga actgctggaa
    50461 aaactggcga ttgccccgga aaatgtggct tatgtcggcg atgatctcat cgactggccg
    50521 gtaatggaaa aagtgggttt aagcgtcgcc gtggccgatg ctcatccact gttgatcccg
    50581 cgcgccgatt atgtgacgcg cattgctggc ggtcgtggcg cagtgcgaga agtttgcgac
    50641 ttattactcc tggcgcaggg caaactggat gaagccaaag ggcaatcgat atgagtaaag
    50701 ccagacgttg ggttaccatt gtgctatcac tggcggttct ggtgatgatc ggcattaata
    50761 tggccgaaaa agacgatacc gcccaggtgg tcgtcaacaa caatgatccc acctataaaa
    50821 gcgagcatac ggacacgctc gtctataacc cagaaggggc actaagctat cgattgattg
    50881 ctcaacacgt tgaatattat tccgatcagg ccgtttcgtg gtttacgcag ccggtactta
    50941 ccacgtttga taaggataaa atcccgacat ggtccgtaaa agcagataaa gccaagctga
    51001 ccaatgaccg gatgctctat ttatatggac acgttgaagt caacgcactt gtgccggact
    51061 ctcaacttcg cagaatcacg acggataacg cgcagatcaa tctggtgacg caggatgtta
    51121 cctctgaaga cctcgtcacg ttatacggaa caacatttaa ctccagcggt ctgaaaatgc
    51181 gcggcaactt acgcagcaag aacgccgagc tgattgaaaa ggttagaaca tcctatgaaa
    51241 ttcaaaacaa acaaactcag ccttaatctt gtgcttgcca gctcacttct ggccgccagc
    51301 attccggcat ttgccgtaac cggagacact gatcagccga tccacattga atcggaccag
    51361 caatctcttg atatgcaagg caacgtggtt acctttaccg gtaatgtcat cgtcacccag
    51421 ggcaccatca aaattaatgc cgacaaagtg gtcgttaccc gtccgggcgg cgaacaaggt
    51481 aaagaagtga ttgacggcta cggtaaaccg gcaacgttct accagatgca ggacaacggt
    51541 aaacccgttg aaggtcatgc ttcccagatg cactacgaac tggcaaaaga ttttgtcgtt
    51601 ctgacgggta atgcttatct gcagcaggtc gatagcaaca ttaagggcga taagatcact
    51661 tacctggtga aagagcagaa aatgcaggct ttcagcgaca aaggcaagcg cgtaacaacc
    51721 gttctggtgc cgtcgcagct gcaggacaaa aacaacaaag gccagacccc ggcacagaag
    51781 aagggtaatt aattcgttat ggcaacatta actgcaaaga accttgcaaa agcctataaa
    51841 ggccgtcgcg tggtagaaga cgtcagcctg accgtcaact ccggggaaat tgtcggtctg
    51901 ctggggccaa acggtgccgg taagaccacc actttctata tggttgttgg cattgtgccg
    51961 cgcgatgcgg gcaacatcat tattgatgat gacgatatca gtctgctgcc tctgcatgca
    52021 cgcgcgcgcc gcggtatcgg ctatctgcca caggaagcct ccattttccg tcgcctcagc
    52081 gtttacgata acctgatggc ggtactgcaa attcgtgacg acttgtctgc tgaacaacgt
    52141 gaagaccgcg cgaacgagct gatggaagag tttcacattg agcacctgcg tgacagcatg
    52201 gggcagtcac tctccggggg tgaacgtcgc cgtgtagaaa ttgcccgcgc actggctgcg
    52261 aatccgaaat ttattctact cgacgaaccg tttgccgggg ttgacccgat ctcggttatc
    52321 gacattaaac gcatcattga acacctgcgc gacagcggcc tgggcgtact gatcactgac
    52381 cacaacgtgc gtgaaacact ggcggtttgt gaacgcgctt atatcgtcag tcaggggcat
    52441 ttgattgccc acggcacgcc tacagaaatc ttacaagacg aacacgttaa gcgtgtatac
    52501 cttggggaag acttcagact ctgatagggt agaagtttgc gacgttttag caggagagta
    52561 cgattctgaa catgaagcaa ggtttgcaac tcaggcttag ccaacaactg gcgatgacgc
    52621 cacagcttca acaggcgatt cgtctgttgc agttgtcgac gctggaactt cagcaggagc
    52681 tacagcaggc gctggagagt aatccgctgc ttgagcaaat cgacactcat gaagaaatcg
    52741 acaccagcga aacgcaagac agtgaaacgc tggacaccgc cgacgcgctc gaacaaaaag
    52801 agatgccgga agagctgccg ctcgatgcca gttgggacac catttacacc gctggtacac
    52861 catccggcac cagcggtgac tacattgacg atgagctgcc ggtctatcag ggcgaaacga
    52921 cgcagacctt gcaggattac ctgatgtggc aggtcgagct gacaccgttt tccgacactg
    52981 accgcgctat tgctacctct atcgtcgatg ccgttgatga caccggttat ctgactgtcc
    53041 cgctggaaga tattctcgaa agtatgggcg atgaagagat cgacatcgac gaggttgaag
    53101 ccgtccttaa gcggatccaa cggtttgatc cggtcggtgt agcggcaaaa gatctgcgtg
    53161 actgcctgct aatccaactc tcccaattcg ataaaaccac gccgtggctg gaagaggcca
    53221 gactgatcat tagcgatcat ctcgatctgt tagccaatca cgacttccgc actttaatgc
    53281 gcgtcacgcg tctgaaagaa gatgtgctga aagaagccgt caatctgatc cagtcgctcg
    53341 atccgcgccc cgggcagtcg atccagactg gcgaacctga gtatgtcatt ccagatgtgc
    53401 tggtgcgtaa gcataacggt cactggacgg tagaactcaa cagtgacagc attccgcgtc
    53461 tgcaaatcaa ccagcactac gcctcgatgt gcaataacgc gcgcaacgat ggtgacagcc
    53521 agtttatccg cagcaatctg caggatgcca aatggttgat caagagtctg gaaagccgta
    53581 acgatacgct actgcgcgtg agtcgctgta tcgttgaaca gcagcaagcc ttctttgagc
    53641 aaggtgaaga atatatgaaa ccgatggtac tggccgatat cgcccaggct gtcgaaatgc
    53701 atgaatcgac gatatctcgc gtgaccacgc aaaaatacct gcatagtcca cgaggcattt
    53761 ttgaactgaa gtatttcttt tccagtcacg tcaataccga gggcggcggc gaagcttcct
    53821 ccacggcgat tcgtgcgctg gtgaagaaat taatcgcggc ggaaaaccca gcgaaaccgt
    53881 tgagcgacag caagttaacc tctttgctgt cggaacaagg tatcatggtg gcacgccgca
    53941 ctgttgcgaa gtaccgagag tctttatcca ttccgccgtc aaaccagcgt aaacagctcg
    54001 tttgacccaa ccgataagga agacactatg cagctcaaca ttaccggaaa taacgtcgag
    54061 atcaccgagg cactgcgcga atttgttaga gccaaatttg ccaaacttga gcaatatttt
    54121 gaccgaatca accaggtcta tgttgttctg aaagtggaga aagtcaccca cacctcagat
    54181 gcaacactgc atgtaaacgg tggtgaaatt catgccagcg cggaaggtca ggacatgtac
    54241 gctgccattg atggcttaat tgataagctg gcacgccagt tgaccaaaca taaagataaa
    54301 ctgaaacaac actaattgtc cgggcaatta gcatgtgcat ggcggcctgt tgtgcggcac
    54361 aacgggccat ttgtacggtt aatgctccga gcctgttcca ctgtttgagt gggcaggttc
    54421 ttaggtgaaa ttatgacaaa taatgataca actctacagc ttagcagtgt tcttaacagg
    54481 gaatgtacgc gaagccgcgt tcactgtcag agcaaaaaac gcgccctgga aatcatcagc
    54541 gagctggcgg cgaaacaact tagcctgccg cctcaggtgg tttttgaagc tatcctgacg
    54601 cgggaaaaaa tgggcagtac cggtatcggc aatggtattg ccattccgca tggcaaactg
    54661 gaagaagata ctctgcgcgc cgttggcgtt ttcgttcagc tcgaaacgcc tatagctttc
    54721 gatgccatcg acaaccaacc ggtggactta ctttttgccc tgctggtgcc ggcagaccaa
    54781 actaaaacgc acctgcatac tctgtcgctg gtggcgaaac gtctggcgga caaaaccatc
    54841 tgccgccgtt tacgcgcagc ccagagcgat gaagagctgt atcaaatcat tacggatacc
    54901 gaaggtactc cggatgaagc gtagttattc ggtaatgtct cttttagacg ttgtgaggag
    54961 aaacagtaca tggtactgat gatcgtcagc ggacgttcag gttcaggtaa atctgtcgcc
    55021 ctgcgtgcgc tggaagatat gggtttttac tgcgtggata accttcccgt agtgttgtta
    55081 cccgatctgg ctcgaaccct ggccgatcgc gagatttctg ccgccgtcag cattgatgtt
    55141 cgtaatatgc cggagtcacc agaaatattc gaacaggcga tgagtaacct gcctgacgct
    55201 ttctcaccgc aactcctgtt cctggatgcc gaccgtaata ccttaattcg tcgttacagt
    55261 gacacgcgcc gactgcatcc gctttccagc aaaaacctgt cgctggaaag tgctatcgac
    55321 aaagaaagcg atttgctgga gcctctgcgt tcgcgagcgg atctgattgt cgatacctca
    55381 gaaatgtccg ttcacgagct ggctgaaatg ctgcgtaccc gtctgctggg taaacgtgaa
    55441 cgcgaactga ccatggtctt tgagtctttc ggcttcaaac acggtatccc tatcgatgca
    55501 gattacgtct ttgacgtgcg cttcttgccg aacccgcact gggatccgaa actgcgtcca
    55561 atgacaggtc ttgataaacc tgtcgccgcg ttcctcgacc gccacacaga agtacacaat
    55621 tttatctacc agacgcgaag ctatcttgag ctatggttac ctatgctgga aaccaacaac
    55681 cgtagctact tgacggtcgc cattggttgt accggcggga agcaccgttc ggtgtatatt
    55741 gcagagcaac tggcagacta cttccgctcg cgcggtaaaa acgtccagtc acgccatcgt
    55801 acgctggaaa aacgtaaacc atgaccgtca agcaaactgt tgaaatcaca aacaagctgg
    55861 gcatgcatgc ccggcctgca atgaagctgt ttgaattaat gcagggtttt gacgctgaag
    55921 tgctgttacg taatgacgaa ggcaccgagg ccgaagccaa cagcgttatt gcgctgctga
    55981 tgttggattc tgccaaagga cggcagattg aagttgaagc gaccggccca caggaagagg
    56041 aagcactggc cgccgttatc gccctcttta actctggttt tgatgaagat taatcttcat
    56101 cactttgaca tacaactccc ttcaaactcc ccccgacaat aagaaaatca cgtactgaaa
    56161 tcgttctcaa tcaacgtcat ttgtacattt tgtgcgcttt tcactttcag aagaacctta
    56221 agaaaacctt aagaggcatt gtttaggttt tgtttaagtt aatcgaccat actggagatc
    56281 gtcagaaaat atttccagga gatggcatga ttcgcttatc tgaacaaagt cccctgggca
    56341 ccggaagaca tcggaaatgt tatgcgcatc cggaagatgc ccaacgctgt atcaagattg
    56401 tctaccatcg tggcgatggt ggcgataaag agatccgccg tgagttaaag tactacgcgt
    56461 atcttggtcg ccggttaaaa gactggagtg gaataccgcg ctatcacggt accgttgaga
    56521 cggattgcgg aaccggttat gtctacgatg tgattgcaga ttttgacggc aaaccctcca
    56581 ttacgctgac cgaatttgct gaacaatgcc gttacgaaga agacattgca caactgcgcc
    56641 agctactgaa acagctgaag cgttatttgc aggataaccg tatcgtgacg atgtcgctga
    56701 agccgcagaa tatcctctgt caccacatca gtgagtcaga ggttatcccg gtcgtctgcg
    56761 acaacattgg tgaaagcaca ttgattccac tggcgacctg gtcgaagtgg tgttgtctgc
    56821 gtaagcaaga gcggttatgg aaacgattta tcgcccaacc ggcgctggct attgccctgc
    56881 agaaagatct gcaacctcgt gagagcaaaa cgctggccct cacttcgcgc gaagcttaat
    56941 ccagctggtg ttgttgcata aacggctcac cgcctaactg atacatctgc cgtaaaatcc
    57001 acgcctgacg gctacgcacg tagcccgatg gcgaggagac tttgaaacga agtggattag
    57061 gtaatacagc tgccagtaat gcagcttccg accgggtaag tttgctcgcg ggtttgtgga
    57121 aataacgttg tgccgcagct tcgacgccaa acacaccgtc gccaaattcg gcgatattca
    57181 ggtaaaccgt caggatacgc tttttgctcc agactgtttc tatccccagc gttaatccag
    57241 cttccagccc ttttcgtacc cagctacgcc catcccataa aaagagattt ttggccgtct
    57301 gctgagaaat cgttgaagca ccgcgaatac ggttttcatt gcgctcgttg tgcgccaggg
    57361 ctttctcaat ggaagcgaca tcaaagcccc ggtgctcagg aaatttctga tcttctgcgg
    57421 caatcaccgc cagtcccatc cacggcgaga tttgatccat actgacccag tcagaatgtg
    57481 ccacgtaacg aaaattgcca tgcagccagg cgctgacctg tcgctcgacc attaccgctg
    57541 agaagggaac aggcgcaaca ctaaacaacg cgatgccccc gccccagaaa acggcgagga
    57601 caaccattaa ccgcaaaaga aaacggcgaa cgaaactaaa caccgttaag cggcttttac
    57661 tcattcagcc agaaccagca cgcgggaaac cagcttatca atgccgctcg ccgcttctgc
    57721 aatgttctgc gccagcatat atgctggggt ggtgacaatc ttattgtctt catcaaccac
    57781 gatatcatcg acaggacagg gcacatgctc cgcgcccatc tcttccagca cttctgcggt
    57841 atcgatatca gtaccgatgg tcaaacgcag cgggaaatcg aaaattttcg gcagcatcgc
    57901 cggggcaata cacataaaac caagcggttt tccggcctga tgcatcgctt gtgccagcgc
    57961 ctttaattca cggtcaacgg tgcattcgct accaagagtg gcaaaattgc ttaaattctt
    58021 cgccgcgcca aacccccccg gcacaatcaa cgcatccagt tcagcggcat cggcctgggc
    58081 cagaggacgg atttcaccgc gcgttattcg tgccgcttca atcagcacgt tgcgcgtttc
    58141 cgtcatcgct tcgccagtta aatggttgat aacatcaacc tgctgcttat ccggtgcaaa
    58201 gcagaccgcc tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg cttcatgaat
    58261 ttcagaaccg tcatagacgc cgcatccgct cagaattacg ccaattttct tcattgtgat
    58321 catcctttcg caattgactg aaacacatta atttttttaa taaaaatggt acgcatcaca
    58381 catttaactg attcatgtaa caaatcattt aagttttgct atcttaactg cgtgcggcct
    58441 gaaaaacagt gctgtgccct tgtaactcat cataataatt tacggcgcag ccaagatttc
    58501 cctggtgttg gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt ttagtggctt
    58561 ttgccaccca cgctttcagc acttctacgt cgtgacgcca ctcttctttc atctcttcaa
    58621 tccattcacc gacgttatct tcccaggcag gaaggtcagg agactgaatt tgctgaccca
    58681 gctgttgcag atggcgtaac cccactgacc ccgccgcacc tttaatttta tgtccttcct
    58741 caacaatgcc ttttttatcc tgcgccgtca gattcgactc cagcacgctg acatagcccg
    58801 gcatcatctt ctcaaacacc gctaacccgt cggtgatcag cttcggtcct acaagttcga
    58861 gatactgttc cagcatggga atatcgagca atgcttctga tttactgttc tcttctgtcg
    58921 tcaccgtact ctcctcatca tcctgggtat cccagaattt cttgatcatc gcggttagcg
    58981 ccggaaccga aagcggctta ctcagcacat catccattcc agcattgagg tactcttgtt
    59041 tgtctttcag cacgttagcg gttaaggcca ccagcggcgg taaatcctcg cgcggataac
    59101 gcttcgtcag ttcacgagag atatccagcc cggtcatatc tggcaactga atatccagca
    59161 acaccaggtc gtattcgccc ggtttaaaca tctccagcgc cgccttgccg gtcatggcga
    59221 catcaacgct gttacctaat ttttccagca cagaacgcgc aacaatcacg ttcagttcaa
    59281 tgtcttccac cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca tcaaacgcat
    59341 catcgacctc ttctgctacc gacggtgcgt ggatcgtcaa cgtaaaggtt gaacctttgc
    59401 cctgttcgct ggtaaccgta atatcgccgc ccatattttt cgccagacga cgagaaacgg
    59461 ccagaccaat accggtgccg gtggcaggtt taccgccatg actgtctttc acctggtaat
    59521 acatggcgaa aattttatcc agctcatcct gcggaatgcc gataccagag tcttccactt
    59581 caaaatgcag catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg ccttgctggg
    59641 tgaatttgac ggcgttactg atgaggttcc acaggatctg ccgtaaacgc gtcccgtcgg
    59701 taatgacctg atgcggtaat ggcagcgtcg gctccaggtt aaagcgcaat cctttttgtt
    59761 gcgcctgcaa tgcggagaga ttttccagat cggcaaggaa gctggtgaaa tcaaccggtt
    59821 gattatcaag ctggaccttg cgccgttcca tcttatccat gtcgataata tcgttaaaga
    59881 tattccccag cgtgacggcc gaaacatgga tggtcttgag atatttttcc tgctcggcgg
    59941 tgagttcggt atccagcaga atgcggctca gaccgacgat accgttcagc ggtgtacgca
    60001 attcgtgact gatggtggag ataaacgtcg ttttgtcgcg gctggcccgt tcaagcgcat
    60061 cctgataccg cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg tgacgtttac
    60121 ccacgcggtc gtagtacggc actttacgga tttcaaagca ggctttgcgc ccgtccgggt
    60181 aatccagcca ctgttcatag gtcagtgaca cattatgacg gaacactttt tcatcggttt
    60241 caatgacttt tgcggcggct tccggtgagt aaacatcagc aggtttcagg tgaaccagtt
    60301 gtttttcgct ttttccggtc agcagctcca tcgcgcggtt acagccggaa aactctttat
    60361 cttcgttacg ataaaaaacc aggtcgggtg aagcatcaag gaaggaacgt aagaatgagg
    60421 attgctgctc gagctgaatt tgtgtctctt cgcgctcttt gatttcaatt ttcagttggc
    60481 cgaaggtttc ctgcagttct gcttccgctt tttcacgaac ggcaatttcc tgatttagct
    60541 gggcaatatt atcttttaac tgaacgttga ggctcaaatc gcgctcgcgc atctcctcca
    60601 gtttttgcac cagccgtgac agacgttgtc gtgactcctc cagttgctcg acgaccaccg
    60661 atagaaagta gaccgcccac ggcgtaatca gcaaaccaaa gaagatagaa cgaataacat
    60721 caatgctttc gacctgacca tgcagcacca tggttaccgc catttgtacc acaatggcaa
    60781 gaacgacgag ggccagcgcc agcaacattg agaagcgcac cagacctaac ttcatcatca
    60841 ggtcaacata atactgcgcc agcagacgaa tttgcttcat tagggaattc cttcacgaca
    60901 acctgcccca ataataccca attctgacag ttacgttata aattgtgtga gaaatgcgga
    60961 attcccctca acgacctact ccgttggagg tagccacgga cgtcccagcg ccgatccctg
    61021 tacgccatgt tcattcagat agcgatccag ctcgaccatt cccgtccatc gattttcgca
    61081 ccacagcgga gcaagcagcg tcgggcgacg ggcgctggcg gaaatgcggt ggtagatcac
    61141 ttccggtggc gtatggcgaa tcatttctcc ggcagtgagc gtgtaatcct ccagttcaat
    61201 accgttcaaa cgccccgctt cccaggcttt cgccataatg ctgcctttca caatatgcag
    61261 cggatgcagc tttatgccgt ccacgccggt ttcaaccact cgctccagcg tttgcaaaca
    61321 ttcagcctgc ccttcaccag gcaggccgac aattaagtgg gaacacacct tcagcccgcg
    61381 ctgacgtgcc agctgggttg tacgctgata acaggcaaaa tcatggccgc ggttgatgcg
    61441 atgcagtgtt ttgtcgtggg cggtttgtag ccccagctcc agccacactt cgtaaccctg
    61501 gtccttatat tcgcaaagca gatccagcac cgcatccggc acgcagtccg ggcgggtacc
    61561 aacacacaaa ccgacaatat tggcctggct caccgcctgc tgatacatag aacgcagcac
    61621 ctgaacttcc gcaaaggtgc tagtatacgc ctgaaagtag gccagatagc gtttagcgcg
    61681 gttaactaaa ttcgcctggt gcgccagttg ctcggcaatg gaacgatgct gctgcgcttc
    61741 atcggcaaac gaggcaacat tacagaatgt gcagccacca cgcccgatgg taccgtcacg
    61801 gttagggcag ctaaaaccgc cgtgcagcgt cagcttatgc accttttgcc cataacgacg
    61861 ggtgagatca ccaccaaaca tattgactaa tttctgtaac tgcataatct gatagaccgc
    61921 gccttgaaaa gaggccaaag cctgccattt ttagctcaat tcggcgatga cctggatcaa
    61981 tcgtcccggc ctgcttttat caactgcata atcaatcaaa attaccgaaa tttcatgcat
    62041 aatcacataa atcacttttg cttatcttgt gtcagatttt tttatctcct tatggatttt
    62101 aggcaaaaac agtagcatga aacgtcatta ccaattaagg cagtataaaa tgctggtttt
    62161 ctcgtcagtt caaggtagga taagggttaa cacgccttta tgacagtcag gaattgactg
    62221 tttctctaac gacttccctt ttagccttaa agataaaatt cattttaatt tcagttgctt
    62281 aataaagaat tttgcgctaa agcacatttc tgtaccaata agcttgccat ttgacctgta
    62341 tcagctttcc cgataagttg gaaatccgct ggaagctttc tggatgagca gcctgctcat
    62401 catatttatg cagtaattga gatcccctct tcaccgtatt aaccgatgcg aaaaggacaa
    62461 caagggggcg aatacgaggc gcgcgtatga cacgcaaccc ccgtcgccac gctctttctg
    62521 tgcccgtgcg cagcggttcg gaagtggggt tcccgcagag cctgggggag gttcacgata
    62581 tgttgtacga taaatccctt gagagggata actgtggttt cggcctgatc gcccacatag
    62641 aaggcgaacc tagccacaag gtagtgcgta ctgcaataca cgcactggcc cgcatgcagc
    62701 accgtggcgc gattctcgcc gatggtaaaa ccggtgacgg ttgcggcttg ctgttacaaa
    62761 aaccggatcg cttttttcgc atcgttgcgc aggagcgcgg ctggcgttta gcaaaaaact
    62821 acgctgtcgg gatgctcttc ctgaataaag atcctgaact cgcggctgcc gcacgccgca
    62881 tcgttgaaga agagctgcaa cgcgaaacct tgtcgattgt gggctggcgt gatgtcccca
    62941 ctaacgaagg cgtgctgggt gaaatcgccc tctcctctct gccccgcatt gagcaaattt
    63001 tcgttaacgc tccggcaggc tggcgtccgc gggatatgga gcgccgtctg tttatcgccc
    63061 gccgccgcat tgaaaagcgt ctcgaagccg acaaagactt ctacgtctgt agcctgtcga
    63121 atctggtgaa catctataaa ggtctgtgta tgccggcgga tctgccgcgc ttttatctgg
    63181 atcttgcgga cctgcgtctg gaatcggcca tctgcctgtt ccaccagcgc ttctccacta
    63241 acaccgtacc gcgctggccg ctggcgcagc cgttccgcta cctggcgcat aacggtgaaa
    63301 tcaacaccat caccggtaac cgccaatggg cgcgtgcgcg tacctataaa ttccagacgc
    63361 cgcttatccc tgatctgcac gacgccgcac cgttcgtcaa cgaaaccggc tctgactcca
    63421 gttcgatgga taacatgctg gaactgctgc tggcaggcgg gatggatatc atccgcgcca
    63481 tgcgtctgtt agtgccaccc gcctggcaga acaacccgga tatggacccg gaactgcgtg
    63541 ccttctttga ctttaactcc atgcatatgg agccgtggga tggcccggcg ggcatcgtga
    63601 tgtccgacgg tcgttttgcc gcctgtaacc tcgaccgtaa cggtctgcgt ccggcgcgct
    63661 acgtcatcac caaagataag ctcatcacct gcgcctctga agtcggtatc tgggattacc
    63721 agcctgacga agtggtcgaa aaaggccgcg tcgggccagg cgaactgatg gttatcgaca
    63781 cccgcagtgg gcgtattctg cactcggcag aaaccgatga cgatctgaaa agccgccatc
    63841 catataaaga gtggatggag aaaaacgtcc gcagactggt accgtttgaa gatctgcccg
    63901 atgaagaagt gggtagccgc gaattggacg acgacacgct tgccagctac cagaaacagt
    63961 ttaactacag tgcggaagag ctggactccg taattcgcgt actgggcgaa aacggtcagg
    64021 aagcggtcgg ttcgatgggc gatgataccc cattcgccgt gctctccagt cagccgcgca
    64081 ttatttacga ctacttccgc cagcagtttg cccaggtgac taacccgcca atcgacccgc
    64141 tgcgtgaagc gcatgttatg tcgctcgcca ccagtatcgg tcgtgaaatg aacgtctttt
    64201 gcgaagcaga gggccaggcg caccgtttaa gctttaaatc gccgattctg ctctactccg
    64261 atttcaaaca gctcacgacg atgaaagagg agcactaccg cgcagatacg ctggatatca
    64321 cctttgacgt cactaaaacc acgctcgaag cgacagtcaa agagctgtgc gacaaagccg
    64381 aaaaaatggt acgtagcggc accgtgctgc tggtgctctc cgaccggaat atcgctaaag
    64441 atcgcctgcc ggttccagcc ccgatggcgg ttggcgcgat ccagacccgt ctggtcgatc
    64501 aaagcctgcg ttgcgatgcc aacatcatcg tcgaaaccgc cagcgcccgc gatccgcacc
    64561 acttcgccgt gttgctgggc ttcggcgcga cggctattta tccatacctt gcctatgaaa
    64621 cgctgggccg cctggtagac acccatgcga ttgccaaaga ttatcgtacc gtgatgctca
    64681 actaccgtaa cggcatcaac aaaggcttgt acaaaatcat gtccaaaatg ggcatctcca
    64741 ccatcgcctc ttaccgctgc tcgaaactgt ttgaagcggt cggtctgcac gatgatgtag
    64801 tgggcctgtg cttccagggg gcggtcagcc gcattggtgg ggcgagcttt gaagacttcc
    64861 agcaggatct gctgaacctg tcgaaacgtg cctggctggc gcgtaagccc atcagccagg
    64921 gcggcctgct gaaatacgtc cacggcggcg aataccacgc ttacaacccg gacgtggtgc
    64981 gcacgctgca acaggcggta caaagcggcg agtacagcga ctatcaggaa tacgcgaagc
    65041 tggttaatga gcgtccggca accacgctgc gcgatctgct ggcaattacg ccgggtgaaa
    65101 acgcggtcaa cattgctgat gttgaaccgg caagcgaact gtttaaacgc tttgataccg
    65161 ccgcgatgtc tatcggcgcg ttaagcccgg aagcccacga ggcgctggcg gaagcgatga
    65221 atagcatcgg cggtaactcg aactccggtg aaggcggcga agacccggcg cgctatggca
    65281 ctaacaaagt gtcgcgcatc aagcaggtgg cttctggtcg cttcggcgtt actccggcgt
    65341 atctggtcaa tgccgacgtc attcagatta aagtcgccca gggcgcgaag ccgggcgaag
    65401 gcggtcagtt gccgggcgac aaagtcactc cttacatcgc caaactgcgc tattcggtgc
    65461 ccggagtgac gctgatctcc ccgccgccgc accacgatat ctactctatc gaggacttag
    65521 cgcagctcat tttcgacctc aagcaggtta acccgaaagc gatgatctcc gtgaagctgg
    65581 tttccgaacc gggcgtaggc accatcgcga ctggcgtggc aaaagcttat gccgatttaa
    65641 tcaccatcgc aggctatgac ggtggcaccg gcgcaagccc gctgtcgtca gtgaaatacg
    65701 caggttgccc gtgggagctg gggcttgttg aaacccagca ggcgctggtt gccaatggtc
    65761 tgcgtcataa gatccgtttg caggtcgatg gcggcctgaa aaccggtgtt gatatcatca
    65821 aagcggcgat tctcggcgca gaaagcttcg gcttcggcac tggcccaatg gtcgcgctcg
    65881 gctgtaaata tctgcgtatt tgccacctga acaactgcgc aacgggtgtg gcaactcagg
    65941 atgacaaact gcgtaagaat cactatcacg gcctgccgtt caaggtgacg aattactttg
    66001 agtttatcgc ccgtgaaacc cgcgagctga tggcacagct gggggtaacg cggctggtgg
    66061 atctgattgg tcgcaccgac ctgctgaaag agctggacgg tttcaccgcc aaacagcaga
    66121 aactggcgct gtcgaagctg ctggagaccg ccgaaccgca tccaggtaag gcactctact
    66181 gcaccgaaaa caacccgccg tttgataacg gcctgctgaa cgcgcagttg ctgcaacagg
    66241 cgaaaccgtt tgtcgatgaa cgccagagca aaaccttctg gttcgatatc cgcaataccg
    66301 atcgctccgt gggcgcgtcg ctttcaggct atatcgccca gacgcacggc gatcaggggc
    66361 tggcagccga tcctatcaaa gcgtacttca acggcaccgc aggccagagt ttcggtgtat
    66421 ggaacgcggg cggcgtggaa ctgtacctga ccggggatgc caacgactat gtcggtaaag
    66481 gcatggcggg cggcttaatc gccattcgtc ctccggttgg ttccgccttc cgcagccatg
    66541 aagcaagtat tatcggcaac acctgcctgt atggcgcgac cggtggtcgt ctgtatgccg
    66601 caggccgcgc gggtgaacgt ttcggcgtgc ggaactccgg tgctatcacc gtggtagaag
    66661 gcattggcga caacggctgt gaatatatga cgggcggtat tgtctgtatt ctgggtaaaa
    66721 ccggcgttaa cttcggtgcg ggcatgaccg gcggcttcgc ttacgttctc gatgaaagcg
    66781 gtgatttccg caaacgcgtt aacccggaac tggtcgaggt cttaaacgtt gacgatctgg
    66841 cgatccatga agagcatctg cgcggtctta tcaccgagca tgttcagcat actggctctc
    66901 agcgcggtga agagattctg gcgaactggt caaccttcgc cactaaattt gcgctggtta
    66961 aaccgaagtc cagtgatgta aaagcactgc tgggtcaccg tagtcgtagc gcagcagagt
    67021 tgcgcgtgca ggcgcagtaa ggggtagcaa caatgagtca gaatgtttat caatttatcg
    67081 acctgcagcg cgttgatccg cctaagaaac cgctgaagat ccgcaaaatt gagtttgttg
    67141 aaatttacga gcagttttcc gaaggccagg ccaaagcgca ggctgaccgc tgcctgtcgt
    67201 gcggcaaccc atactgcgag tggaaatgtc cggtacacaa ctacatcccg aactggctga
    67261 agctcgccaa cgaggggcgt atttttgaag cggcggaact gtcgcaccag accaacaccc
    67321 tgccggaagt ttgcggacga gtctgcccgc aagaccgtct gtgcgaaggt tcctgcactc
    67381 tgaacgatga gtttggcgcg gtgaccatcg gcaacattga gcgttatatc aatgataaag
    67441 cgttcgaaat gggctggcgt ccggatatgt ccggcgtgaa gcaaaccggt aaaaaagtgg
    67501 cgattatcgg cgcaggcccg gcaggtctgg cgtgtgcgga tgtcctgacg cgtaacggcg
    67561 taaaagccgt tgtcttcgac cgtcatccag aaattggcgg gctgctgacc ttcggtattc
    67621 cggccttcaa gctggaaaaa gaggtaatga cgcgtcgccg tgaaatcttc accggcatgg
    67681 gtattgaatt caaactcaat accgaagtgg gccgcgacgt gcagctggac gatctgctga
    67741 gtgattacga tgccgtcttc cttggcgtcg ggacttatca gtcaatgcgc ggcgggctgg
    67801 aaaacaaaga cgccgatggc gtgtacgcag cgctgccgtt ccttatcgcc aacaccaaac
    67861 agttaatggg ctttggtgaa acccgcgacg aaccgttcgt cagcatggaa ggcaaacgcg
    67921 tggtggtcct tggcggtggc gacactgcga tggactgcgt gcgtacgtcc gtgcgccagg
    67981 gagcgaagca cgttacctgt gcctatcgtc gtgatgaaga gaacatgccg ggttcccgcc
    68041 gcgaagtgaa aaacgcgcgg gaagaaggcg tagagttcaa attcaacgtc cagccactgg
    68101 ggattgaagt gaacggtaac ggcaaagtca gcggcgtaaa aatggtacgt accgaaatgg
    68161 gcgaaccgga tgccaaaggc cgtcgccgcg cggagatcgt tgcaggttcc gaacatatcg
    68221 ttccggcaga tgcagtgatc atggcgtttg gtttccgtcc acacaacatg gaatggctgg
    68281 caaaacacag cgtcgagctg gattcacaag gccgcatcat cgccccggaa ggcagcgaca
    68341 acgccttcca gaccagcaac ccgaaaatct ttgctggcgg cgatatcgtc cgtggttccg
    68401 atctggtggt aaccgctatt gccgaaggtc gtaaggcggc agacggtatt atgaactggc
    68461 tggaagttta agctctgtcc cagcgccgcc tcctcagaaa agagtcggcg ctgaaattct
    68521 cctttctttc tggaaagcgc ctcgcataat ctgaagaaaa ccaatgtaat acgctcatct
    68581 ggattgttac cggtagatgc ccggcgtata gctgattcca ttattcaacg cagcgccata
    68641 taaagggaca gataatggaa tcgctctcag agagaacctc aacaggctac cagcaaatcc
    68701 acgacggtat tattcacctg gtcgatagcg cccggacgga aacggtacgt agcgttaacg
    68761 cgctgatgac cgcgacatac tgggaaattg gccgacgaat tgtcgaattt gaacaaggtg
    68821 gcgaggcaag ggctgcgtat ggtgcgcagc taatcaagcg actatcaaag gatttaagtc
    68881 taaggtataa gcgtgggttc tctgcaaaaa acttacgcca aatgaggctt ttttacctct
    68941 tttttcaaca tgttgaaatt cgccagacag tgtctggcga attaacacca ttgggaattc
    69001 cgcagacacc atctgcagaa ttcccatcgg caaagatttg gcagacgctg tctgccaaat
    69061 tgagaggctg tttgatattt ccacagcttt tgtattgcat ctcctatttt cggtttgtat
    69121 attcatgcaa ataaacgaag gaaagttgcc atgatttaca ttgaacagct tgaattaatc
    69181 cataaaagtg gtgatgtgtt gtatccagta aaaataacca gaaaatcatc aggaaaaact
    69241 gccttccatc ttgttccatt cggcttaaat aagactcacg accttctaga ggtggaagat
    69301 gcttcagaag caattcggct ggtgattgat gaacgtcatt ccattcgctg cagtacactt
    69361 acagcaacta ttacgaataa aaagggcaag cgaattaagc ggacaggtat atacagtatc
    69421 aagggtgtta acataaaaga atacaacgtc aggtaatggc acagagcggt ccgtcagttc
    69481 aggtttggct ctgtgccaaa gatgggtcag gtcacacctg atctaataaa tttaagccat
    69541 taaatcagcc ttaaccttca ccaccacctt tttaatctca cccggctcgc cgacaacgca
    69601 tcctggttta tgcggttcac ttggcataaa cacggcgaac attcccggtt ttaagatgat
    69661 ggcttgctcg ttatcaatgg tgctgcaaag ctgataatca tcctcatggt ggaactcttc
    69721 acactggcgc gcagtgcctg ccatgccaaa cagaatccgt tcctcaccgt ttaataacag
    69781 ctggatatca atgtattgct cgtgcaattc cgcttttttc tcgacgggcg attgagtgtt
    69841 aaacgtcatg acattcataa agatattgtc gccctgtaat tcgtaacgac caggcgcttt
    69901 ttcttgcggt ctggcagcta atgccagcgt taacgcgtcc tgtaacgcag gatgtaaccc
    69961 agcagacggt aatgactgta cttcacccat catcataatt tttctccctg ggccaacagc
    70021 gcagccccaa gtaaacctgc atcgtggcgg tagtgcgccg ccagtaaatc aacatgaaat
    70081 gccgctggct cctgcgccag atacgtttcc accaacgcca gatacccttc tgccagacca
    70141 acgctgccac cgaccaccac gcactggcaa tcagttgtgg ctttaatatc agcgatcagc
    70201 cttgccagca catgtgcgga gcggtgaatc agttgctgcg cctgctcgtc gccctgcccg
    70261 gcgcgtgtga aaatagttct cgcatccgcg cccatgagtt caccttgcgc tgccgctgca
    70321 ataccgcgac cagaagcaat cgcttccacg caacctgtgc gtccacagcc gcagactggg
    70381 ccgtgtggat cggcaagcgt atgcccgatg tgccccgcca gaccgccagg gccggtaagc
    70441 agtttaccgc cgctcactac accgccgccg acgccagtgg aaacggtgat aaacaccata
    70501 tcggttatat cgccttccag cgcctgatac tctgcccatg ctgcggcctg cgcgtcgtta
    70561 atggcaatgg tcggcaaatt ggaaagttgt tccagcgttt tgactaacgg aaagtgtagc
    70621 aatccaccaa gattatgcgg attcagcgcc agcaagctgc cgtcgcgaat aattccggtt
    70681 gaagcgatgg caacctgctg cgcatgagct tgcaacgggg cgactaacgc ggctaaggca
    70741 tcacgcaacg cctgcggagt ctggctggct ggtgtcggaa gttctcggcg atcgcggatc
    70801 tgcccgtcag cgccaatcag cgcggcggca agtttagtac cgccgatatc aatcgccaac
    70861 gtggtcatag caccgccttt ttcatcgctg tgttgtacca ctgacaaatg tgttcaagac
    70921 gcgtaatggc agaaccgacc gtcaccgccc acgcgccgtg gcgcatcgca tctgccgcct
    70981 gagcaggcgt gttgtaacgc ccttcggcaa tcacccgaca tccggcgtcg ctcaacgttt
    71041 tcaccagcgc cagatccggc tcttctggcg tttcaggcgt ggtatagcca gaaagcgtag
    71101 tgccaataat ttcggctccc agcttttggc atgccaggcc gtcttccggc gttgagcagt
    71161 cggtcatcgc cagtaaaccg tgatggtgaa tacgtgccag cagcgtttca acaggcaccg
    71221 gacgcgggcg gtcggtgccg tcaatggcga taatgtccgc gcccgcctgc gccagcgcat
    71281 caacatcttc aatataggcc gtgatgcgta ccggagaatc ctccagatcg cgtttcacaa
    71341 ttccaataat cggcacgctc accaccgcac gcgtggcttg cagatttgcc acaccttcaa
    71401 tgcgaatggc aaccgcgccc gcctgttctg ccgctaatgc catggcagcg acgatttcgg
    71461 gtttatcgag cgggctgtcc ggaaccggct ggcaggagac aatcaggcca ccgttagcag
    71521 cgattttttg atccagttgt gcaagtaacg acatacatct tcccttagcg aaaggcccgg
    71581 tacagagacc gggcaacagg attaactttt ggttttgact aaatcgtttt tggcgctgcc
    71641 aaatggcacg gcaccgctaa atggtttgcc gtcgatagcg tcatgagtac gcaatgcttc
    71701 cgggcgcagc caacgctgaa cgcgggaagg catatccaga ccaatcagca ggatcaccac
    71761 aaatgtcaga ctgaacgata gcgatgccag cgcagtaccc agatccagac gttgagcgat
    71821 caacgcgccg atgattgggg ccagtgcgcc gcccaatgcg ccaacgttgt aggtaaagcc
    71881 caggcccgct gcacgctggt cggtatcgaa ataaccgcca atcagtttcg gtaagatccc
    71941 ggcgatccct tgtccaagca tttgctggaa gaacagtaac agaccgagca cccagacgtt
    72001 tgcgccgcca atcgcaaata ccggaataat caacagctgc gaggccaaca ggctacaaac
    72061 gtaggctttg cgggttccca gccagtcacc gaggaagccg ctgacacagc accccaccgc
    72121 agccccaaag ccgctaaaga acagcacatt ggcgacagta tgcgggttat aagccagatc
    72181 ggttttcaga tacgttggca gcagcgcctg aatcggccat gagtagagga aagcaaacaa
    72241 cacgaccacc atcagcatta cgcccgttgg ccagcgttta cccgtacttt gcaccataaa
    72301 gctgataaag attgcggcgc ataacagccc aagaacagcg acgatcgcgg cattttgcag
    72361 gttaccggcg aagcaaaacc acagcgcagt agccgccgcc agtgtcatta cgatattggc
    72421 aatgcgatgt tcaccacggt agagaatatc taccatcgtg cgtactggtg ctttacctgc
    72481 atgcttctct ttccagtctt ccgcttccgg gatgttttta cgcagccaga gagcaaagat
    72541 gataggcaaa atgccgataa agaacagcgc acgccagccc cagaccggaa ccaccaggct
    72601 atagacctga gcggcaacga cggcccccac agagaagcct gaaatcagaa aaccactggc
    72661 tttgttacgc agatgttttg gccagctttc aatgacatag gtggcgctgg aaccgtattc
    72721 acccgccatc cccatgccga tgaccagacg cgcgataaac atggtgatgt agcctggcgc
    72781 aaagccgcag gccagcgtcc cggccgagaa gagaacgatg ctggtgacca ttgccagacg
    72841 acgcccgtag cggtcaccca tagcgccgag catcaggccg ccgaaccagc gagagataaa
    72901 ggctgcggag atcagacttg ccgcctgcac cgtcgtcagc ccgaattcac cctgtacttc
    72961 ggtgagtacc agggcgatta aaacgaaatc aaaaccgtca agcagatatc ccaaccaggc
    73021 agcggaaaat gcgcgccatt gtgcacggtt gagatggcga taccacggga tattctgggt
    73081 tgtagtactc attgtgagtc tcccgcggtg ggcgatgccc acacgctttg ctataaaaat
    73141 tgtagggtac agatgcgttt atttcccctc acccggtagg ggcgagcgag gggaaacaac
    73201 tcacccgcgc tcttgcatca actgctgggc cagcgccttc agttctggca gatatttttc
    73261 atctaccggt ccaaacggtt tgcggcacag cggcacagaa acgacatcca tataatggag
    73321 gacagttttc aggccgcgga atacgcccgt tttgatcagt aaatcaatga ctttattgca
    73381 ttcagtttgc agtttctgcg cggtctggat atcgccttct ttcagcgcct taacgatccc
    73441 ctgatagcgc cagcccatga tgttgtaggt actaccgata ccaccatcag cgcccgccag
    73501 cagaccagag gcgaagattt cgtcgtaacc gttatagagc acaagatcag gatgttcacg
    73561 acggatctgc tccatctgat agagatcgcc agaggtctgt ttcagcgcac ctacgccagg
    73621 caatgtaaca agtgtgttga tctgatccag ggtcagtttt accccactca gggctggaat
    73681 gttgtacacc accatcggca aaccatccgc cgaatcaata attgcccgat agtgatcgca
    73741 gtgttcttca aagctgaaag gatagtagaa cggcgtgacg gcggagacgg catcgaagcc
    73801 ataacgttta gccgatgccg caagttgttg gctttcggcg gtgctgacgc aaccgacgtg
    73861 ggcgatgagt ttaatcttac ctttcgcctc ttcggcgacg atttccagta cctgttcacg
    73921 ctcggaaagg ctttgtacaa aggcctcgcc ggtcgaacca cccacgtata aaccgtcgat
    73981 gccctgctga atattgaact gaaccaggcg acgcagactc gctttatcca gtgcttgttg
    74041 ttggtcaaaa ggagtcagga gtgcagccat tacgccacgt aaattcgttg ccataaatac
    74101 ctctgaagtg atgcttgtct gataaacgat atacctttat acctgttata ccagatcgat
    74161 taagcaacac cccatacaga aagcttataa tgcgatctgc ttcactaaag tggcattatt
    74221 tctttttgtt ggtggtctga ccgaaagcgt gccaggtagc agagacgctg ttgagatgcg
    74281 attgcaacgc acgatcggct tcgtcaggat catgacggcg gatcgcatca acgatcgcaa
    74341 tatgctgttg ataactaacg ttgttatgtt cgtgcagtgc ctgatcggta accgttgggc
    74401 gtgcggcaat aagccagtcg agcagggcaa cgtggatcgc catgaagatt gggttaccgg
    74461 ggatctccgc cagcacgcgg tggaaatcaa cgtctgaacg aatgaatgcc gcgttgttat
    74521 ccagcgactg actgttgatt tccagtgctt ttgccagcaa atcgatttgc tcatcggtgg
    74581 catgttcagc cgcatagcgc accagactgg attcaaagaa cagacgtaat tgttcgaaat
    74641 gggcaatccc accgggatga gaaaggaaat ctttcgccat gccggaaagc tcaccgatga
    74701 tagtgtccgc agaaggacgc gagacgcgag cgcgttcgcc gttgtttatt tgcaccagac
    74761 ctttgcgttt taacgctgcc agcgcttcac gcaccgaagg acgcccgacg ttaaagaacg
    74821 ccatcagttc gcgttcagac ggtaattgtt caccttcgcc aaattcacga cggcggatca
    74881 tctgttccag ctcttcttcc accatttcgg agagtttttt acgcgccagc gggcggctac
    74941 gcaagttgcg accaattgca ggtgaagaat cttcggtttg cgaatcaaat gcgttcataa
    75001 ggcccattct gtaaggtcag tgtgattaac atcatcagtg acatcctatc acaggattga
    75061 gagtagggga aaatggcagg tttttctctt tgtgcctcat cattaccata attaacggaa
    75121 taattaacta ttgcgaaaaa ttaatgtaac gcagataaaa acatcccgtt tgaattattt
    75181 ataagactat tcacgagcat tatgaatatt atgaatgcgt tcttgcaaaa taatcataag
    75241 cgcatatttt ttaatgaaaa attatctcac ctacaattaa aaacacgaca tccgcaccat
    75301 aaatagactt gcaaaaaata taacatcatt gttttcaatc tgccgtttat gggattgacc
    75361 gttttctttt gacacggagt tcaacaatgt tcggcataat tatatctgtc atcgtattaa
    75421 ttacgatggg ctatttgatc ctgaaaaact acaaacctca ggtggtgctg gctgccgcag
    75481 gtatcttcct gatgatgtgc ggtgtctggt tagggttcgg tggtgtactc gagcccgcca
    75541 aaagcagcgg ctacttgatc gtcgatattt ataatgaaat cctgcgcatg ctgtccaacc
    75601 gcattgccgg attggggcta tcgattatgg cggtgggcgg tatgcccgct acatggagcg
    75661 cataggggcc agtcgcgcga tggtgagctt gttaagccgc ccgttaaaac tcattcgctc
    75721 accgtatatt attctgtcgg caacttacgt catcggtcaa atcatggcgc agtttattac
    75781 cagcgcctcc ggtctgggta tgttgctgat ggtcacctta tttccgacac tggtgagtct
    75841 gggagtaagt cgtctctctg cggtggcggt tatcgcaacc acaatgtcca ttgagtgggg
    75901 aattctggaa acgaactcca tttttgcagc ccaggtcgcg ggaatgaaaa ttgccactta
    75961 cttcttccac taccagcttc cggtcgcctc ttgcgtcatt atctcggtgg cgatctccca
    76021 ctttttcgtg caacgcgctt ttgacaaaaa agataaaaat atcaatcacg aacaagcaga
    76081 gcaaaaagct ctcgataatg tcccgccgct ctattacgcc attttacctg tgatgccgtt
    76141 aatcctgatg ctcggctcgc tgttcctcgc ccacgtcggg ctgatgcagt cagaactgca
    76201 tctggtggtg gtgatgttac tgagtttgac ggtgacgatg tttgttgagt tcttccgcaa
    76261 gcataacttg cgcgaaacaa tggacgatgt gcaggcgttt tttgacggca tgggtacgca
    76321 gtttgccaac gtggtaacgc tggtggtcgc gggtgaaata tttgcgaaag gcttaacgac
    76381 gattggcact gtcgatgcgg ttatcagggg tgcggagcat tctggtctgg gcggtattgg
    76441 cgtgatgatt attatggcgc tggtcattgc catttgtgcc attgtgatgg gctctggcaa
    76501 tgcgccgttt atgtcatttg ccagtcttat tccgaatatc gcagccggac tacatgtacc
    76561 agcggttgta atgattatgc cgatgcattt tgccacgacg ctagcgcgcg cggtttcgcc
    76621 gattactgcg gtggtggtcg ttacgtcagg aattgcaggc gtttcgcctt ttgcggtggt
    76681 gaagcgaaca gcgatcccca tggcagtcgg tttcgtggtg aatatgattg ccacaatcac
    76741 gctattttat taagccatta aaagacaaaa caggccacca gggcctgttt ttgtattact
    76801 tcacaacgcg taatgccggt cgaccaccgc gtggtggctg cggaggttca tcgtcaggat
    76861 gagtgtcatc atcgtgatct ggcttgtcgc catcaataac cgacataacg gtttcgttgt
    76921 ctgccgatgc ctcttcatca ttcatgatgc tggtatcttc atcgtaggca gcttcaggct
    76981 caaacattgt gcctgcgcca ttttcacggg cgtagatagc cagcacggca gccagcggca
    77041 cagaaacctg acgcggaatg ccgccaaagc gcgcgttaaa gcgcacctca tcattcgcca
    77101 gttccagatt gccgacagca cgcggcgcaa tgttgagtac gatttgcccg tcacgcgcat
    77161 attccatagg aacctgcacg ccagggagcg tcacatccac cactaggtgc ggcgtgagct
    77221 ggttatccag caaccactca tagaatgcac gcagcagata gggacgacgt ggtgttagct
    77281 gtgacaaatc catacagatt aactccggcc cagacgcatt tcacgttctg cttcagttaa
    77341 agaagcaagg aaagagtcac gctcaaagac gcgggtcata tagcctttca gctctttcgc
    77401 acccgggccg ctgaactcga tgcccagttg cggcagacgc cacagcagcg gagcaagata
    77461 gcaatcgacc aggctgaact catcgctcag gaagtacggc ttctgaccga agaccggcgc
    77521 aatcgccagc agttcttcgc gcagttgctt acgtgcggca tctgcttcag aagctgaacc
    77581 gttgatgatg gtgttcatca gcgtgtacca gtctttttcg atgcgatgca tgtacagacg
    77641 gctttcaccg cgagctaccg ggtaaacagg catcagtggc ggatgcggga aacgctcatc
    77701 cagatattcc ataatgatgc gagattccca cagggtcagc tcacgatcca ccagggtcgg
    77761 aacgctctga ttcgggttga ggtcaatcag atcctgaggc ggattgtcct tttccacgtg
    77821 ttcgatctcg aaacttacac ctttctcagc cagcacaatg cggacctgat ggctatagat
    77881 gtcagtagga ccggaaaaca gcgtcattac cgaacgtttg ttggcagcga cagccatgaa
    77941 aacctccagg tatagtcaga atttttactg ctaccagcca ccaggtggcc agtcagaagt
    78001 tgtgttaccc agtaaggaac gactctcttt gttcgaaaat caaacaaaaa atgagcaata
    78061 cccgacattt gggcagaaaa ttggatgata gtttaccaga ttttgcgacc tttgtggtga
    78121 gtcgatgccg gaaatgggga aaaagagatg cgctttagtc tgaaatagtt gacttagtcc
    78181 cttattggcg atgtggtttt tgttttacct gtctgtcagg tggcagcaaa aagcaacttt
    78241 ccagttttta cgctgattca gattttagct ataaaaaaac ccgccgaagc gggttttttc
    78301 gaaaattgtt ttctgccgga gcagaagcca attaacgttt ggagaactgc ggacgacgac
    78361 gtgctttacg cagaccgact ttcttacgtt caacctgacg agcgtcacga gtaacgaagc
    78421 cagctttacg cagttcagaa cgcagggact cgtcgtattc catcagagcg cgggtgatac
    78481 cgtgacggat cgcaccagcc tgaccagaga taccaccacc tttaacggtg atgtacaggt
    78541 ccagtttctc aaccatgtcg accagttcca gcggctgacg aactaccatg cgggcagttt
    78601 cacgaccgaa gtactgttcc agagaacgtt ggttgattac gattttaccg ttgcccggtt
    78661 tgatgaaaac gcgagctgcg gaacttttgc ggcgaccagt gccgtagtat tgattttcag
    78721 ccattgccta taatcccgat tagatgtcaa gaacttgcgg ttgctgtgcc gcgtggttgt
    78781 gctcgttacc cgcgtaaact ttcagtttac ggaacatagc acgacccagc gggccttttg
    78841 gcaacatgcc tttaaccgcg atttcaatca cacgctcagg acggcgagca atcatctctt
    78901 caaaggtcgc ttgtttgata ccaccgatgt ggccggtgtg gtgatagtac actttgtcag
    78961 tacacttgtt gccggttaca gcaactttgt cagcgttcag aacgatgatg taatcaccgg
    79021 tatctacgtg cggagtgtat tccgctttgt gcttaccgcg caggcgacga gccagttcag
    79081 tagccagacg gcccagagtt ttaccggtcg cgtcaacaac ataccagtcg cgttttacgg
    79141 tttctggttt agctgtaaaa gttttcatta aaagcttacc caataaatag ttacacgttg
    79201 gtgaacaccc aaacgtcttc aattgttgag gttcacacga caaagtccgg caaacctacc
    79261 ccttcgaata gcctatgcca gcacacaaaa agttttggga aaaaactttc ttgtaacgtg
    79321 gggtcgcagg attatagaga agtcggggtc aaagatcgac ccctttttgt gatttgtgac
    79381 aggttttaac ccgccaaatg ctcgcgcttc agatactctt cgctttgcat ctcttgcaga
    79441 cgtgacaggc aacgctggaa ctcaaacttc agccgatcgc cctgataaat ttcatacagc
    79501 ggcacttctg cactcaccac taatttgaca tggcgctcgt aaaactcatc caccagcgca
    79561 ataaagcgcc gcgcttcgct ctccatcaac cgcgtcataa ctggtacatc aaacaacatg
    79621 accgtatgaa agaggcgtga gagcgcaata tagtcatgct ggctgcgggc atcgacacac
    79681 aaggtggtaa aagagacagc aagcgtctga ttctcagcgc ccattgtcgc cagcggtcga
    79741 tggttgattt ctaacgtcgg tgaattttcg cgtttcgctc ccgccagcgc caaccatagt
    79801 ttatccattt gtgcccgggt ttcatcgtgg agtggcgaaa gccacagatg cgcctgagtg
    79861 agtgtacgca gtcgataatc aacaccagcg tccacgttca ttacatcaca atgctgttta
    79921 atggcatcga ttgcaggcag aaaacgcgca cgttgcaagc catttcgata aagttcgtcc
    79981 ggcggaatat ttgacgttgc taccagggta ataccgcggg cgaacagggc tttcatcaga
    80041 ccgccaagta gcatggcgtc ggtaatatca gaaacaaaaa attcgtcaaa acagagcacg
    80101 tcagtttcgg ctttaaagcg atcggcaata atttccagcg gatcggtctg cccctgtaag
    80161 gcagttagct cttcgtgcac ccgcagcata aaacggtgaa agtgcaggcg ctgtttccgc
    80221 tctcccggca ggctttgata gaaaaggtcc atcagccagg ttttcccgcg tcctacaccg
    80281 ccccacatat ataagccacg cactggcgta tgctttgtgt cttcgcgttt accccacagc
    80341 ttaccgaccc gcgccattag cccactcgtc ctgggggctg gtggcctgct attgatgagt
    80401 tcttgataaa taatttccag gcggctgacg gcctcttttt gaacgtcgtc gggttgatgg
    80461 ctgccttcat tgagcgcctt caggtattgc gatgttgggg taacgctttg catgatctta
    80521 ttattattcc ttgaataatc ggtgcgccgt tgttcacggt tgacgaaaaa aaggccgttc
    80581 tacactacgc gatatgcagt cgggattcca cttctgtgga attaacggtt atagtggcat
    80641 aatcagccgt aggcatggag cctgaagcca acaccctacg gaaacaaaag acaacgggag
    80701 atgttcatga cctgggaata tgcgctaatt gggttagtcg tcggcatcat tattggtgct
    80761 gtggccatgc gttttggtaa tcgtaaacta cgccagcaac aggcgttgca gtacgaactg
    80821 gaaaagaata aagctgaact ggacgagtat cgcgaagagc tggttagcca ctttgcccgc
    80881 agcgcggaat tactggatac catggcgcac gactatcgcc agctgtatca gcacatggcg
    80941 aaaagctcca gcagcctgct gccggaactg tctgctgaag caaacccgtt ccgtaatcgt
    81001 ctggcagagt ctgaagccag caacgatcag gcaccggtgc agatgcctcg cgactattct
    81061 gaaggtgcat ccggcctgct gcgtactggc gcgaagcgcg actaatttat ttttcgggcg
    81121 cagccattgc gccctcctct tctctccctc cccgactatc atttaatctg gtgtctcatt
    81181 gttagccgtc tgaaaattca ataacatcaa actgttttga atctcttttc ttatcattca
    81241 ggtacgagag caggaataat gaaaaaacaa acccagctgt tgagtgcatt agcgttaagt
    81301 gtcgggttaa ctctctcggc gtcatttcag gccgtcgcgt cgattccagg ccaggttgcc
    81361 gatcaggccc ctctccccag tctggctcca atgctggaaa aagtgcttcc ggcagtggtg
    81421 agcgtacggg tggaaggaac ggccagtcag ggacagaaaa tcccggaaga attcaaaaag
    81481 ttttttggtg atgatttacc ggatcaacct gcacaaccct tcgaaggttt aggttccggt
    81541 gtcatcatca acgccagtaa aggctatgtg ctgaccaaca accatgtgat taatcaggca
    81601 cagaaaatca gtattcagct caatgatggg cgcgagtttg atgcaaaact gattggtagc
    81661 gatgaccaga gcgatatcgc cctgttacaa attcaaaacc cgagcaaatt aacgcaaatc
    81721 gctattgccg actccgataa attgcgcgtc ggtgattttg ccgtagcggt cggtaaccca
    81781 tttggccttg ggcaaaccgc cacctctggc attgtttccg cattaggccg cagcgggttg
    81841 aatcttgaag gtctggaaaa ctttatccag acagatgctt ccattaaccg cggtaactcc
    81901 ggcggtgcac tattaaacct taacggtgag ttaattggca tcaacactgc aatccttgcg
    81961 cctggcggcg ggagcgtcgg gattggattt gccatcccca gtaatatggc gcgaacactg
    82021 gcgcagcagc ttatcgactt tggtgaaatc aaacgcggtt tgttaggcat caaaggcacc
    82081 gagatgagtg ccgatatcgc caaagccttc aaccttgacg tgcagcgtgg cgcgtttgtc
    82141 agcgaagtgt tgccaggttc tggctcggca aaagcgggcg tcaaagcggg cgatattatt
    82201 accagcctca acggcaaacc gctgaatagc tttgctgagt tgcgctctcg tatcgcgact
    82261 acagagccgg gcacgaaagt gaagcttggc ctgctgcgta acggcaaacc actggaagta
    82321 gaagtgacgc ttgataccag cacctcttcg tcggccagcg ctgaaatgat cacgccagcg
    82381 ctggaaggtg caacgttgag cgatggtcag ctaaaagatt gcggcaaagg tattaagatc
    82441 gatgaagttg tcaaaggaag cccagctgct caggcaggct tgcaaaaaga cgatgtgatc
    82501 attggcgtca accgcgatcg ggtgaactcg attgctgaaa tgcgtaaagt gctggcggca
    82561 aaaccggcca tcatcgccct gcaaattgta cgcggcaatg aaagcatcta tctgctgatg
    82621 cgttaatgtc gtaaaccggg catcaggctt acgtgtgatg tccggttaac tcgtggtatg
    82681 ctgctgccgt tccctttttt aatgacgcct ccatcatgtt tgtgaagctc ttacgttccg
    82741 ttgcgattgg attaattgtc ggcgctattc tgctggttgc catgccttcg ctgcgcagcc
    82801 ttaacccgct ttccactccg caatttgaca gtaccgatga gacgcctgcc agctataatc
    82861 tggcggttcg ccgcgccgcg ccagcggtgg ttaacgttta caaccgtggt gtgaacacca
    82921 actctcacaa ccagcttgag atccgcaccc tgggatccgg tgtaatcatg gatcaacgcg
    82981 gttatatcat caccaataaa cacgtcatca acgacgccga tcagatcatc gtcgccttac
    83041 aggatggacg tgtatttgaa gcattgctgg tgggatctga ctctctaacc gatctggcgg
    83101 tacttaaaat taatgccact ggcggtttac ctaccattcc aattaatgca cgtcgcgtac
    83161 cgcacattgg cgacgtagta ctggcgatcg gtaacccgta caacctcggg cagaccatta
    83221 cccaagggat tattagtgcc acggggcgaa tcggtctgaa ccctaccggg cggcaaaact
    83281 tcctacaaac cgatgcttcc attaaccacg gtaactctgg cggcgcgctg gtgaactcgc
    83341 tgggagaact gatgggcatt aacacgctgt cgtttgataa gagtaacgat ggcgaaacgc
    83401 cggaaggtat cggctttgcg attcctttcc agttagcaac caaaattatg gataagctga
    83461 tccgcgatgg gcgcgtgatc cgcggctaca ttggcattgg tggacgtgag atcgcaccac
    83521 tgcacgcgca gagcggtggt atagatcaac tgcaagggat cgtggttaat gaagtgtcac
    83581 ctgacggccc ggcggcgaat gcgggtattc aggtcaacga tctgattatt tcggtggata
    83641 acaaaccggc catctctgct ctggagacga tggatcaggt ggcagaaatt cgccctggtt
    83701 cggtgatccc ggttgtagtg atgcgtgatg ataagcagtt aacgctgcag gtcaccattc
    83761 aggaatatcc ggcaaccaat taagtcgagt gctcaaaaca aaaaaccgga gtctgtgctc
    83821 cggtttttta ttattcgcta atcaattact tattaacgaa ctcttcgccc agggcgatat
    83881 ctttcttgag cgtatccagc ataccttcca gcgcgttctg ttcaaatgcg ctcagggtac
    83941 cgatagattt acgctcttcc acgccgtttt tacccagcag cagcggttga gagaagaaac
    84001 gggcgtactg accgtcgcct tcaacgtagg cacattcgac aacgccttgt tcgccctgca
    84061 gtgcacgaac cagagacaga ccaaaacgtg cagctgcctg gcccatagac agggttgcag
    84121 acccgccacc ggctttcgct tcaaccacct cagtacccgc gttctggata cgtttggtca
    84181 gatcagccac ttcctgctcg gtaaaactaa cgccaggaac ctgtgacagc agcggcagaa
    84241 tggtaacacc agagtgaccg ccaataaccg gcacttcaac ttcgcttggc tgtttgcctt
    84301 tcagttccgc aacaaaggtg ttggaacgaa tgatatccag cgtggtaacg ccgaacagtt
    84361 tgtttttgtc ataaacaccg gcttttttca gcacttcagc agcaattgca actgtggtgt
    84421 taaccgggtt agtgataata ccaatgcacg ctttcgggca ggttttcgca acttgctgta
    84481 ccaggttttt cacgatgccg gcgttaacgt taaacaggtc ggaacgatcc atacccggtt
    84541 tacgcgctac acctgcagag ataagaacga catctgcgcc ttccagcgcc ggagtcgcat
    84601 cttcaccaga aaaacctttg attttcacag cagtagggat atggctcaga tcgacagcca
    84661 caccgggagt cactggagcg atatcataca gagagagttc tgaacctgaa ggcagttggg
    84721 tttttaacag tagtgcaagc gcctggccaa taccgccagc agcgccgagg actgcgactt
    84781 tcatcctaaa ctccttatta tattgataaa ctaagatatg ttgctccgct gccgcgacct
    84841 taatccacaa aattgccgtt tacaatgacc acatatcaag aatgtgtagt cacgcaagtt
    84901 tagcgtttat gcatttaatt gccgtaatca ggaacctaac ttacgtaatt aacagtcgct
    84961 tatgcattag cgcaacattc caacaggtgg tgacaatata ccctaccgtt cagccaaaac
    85021 aacatcaatt tgataacaat taatttactt ttaagcagaa tttgcatgcc gtgacgcagg
    85081 catgtttctc aataacgaaa tttgataaaa tcccgctctt tcataacatt atttcagcct
    85141 tcttcagggt tgactgtttg cataaaaatt catctgtatg cacaataatg ttgtatcaac
    85201 caccatatcg ggtgacttat gcgaagctcg gctaagcaag aagaactagt taaagcattt
    85261 aaagcattac ttaaagaaga gaaatttagc tcccagggcg aaatcgtcgc cgcgttgcag
    85321 gagcaaggct ttgacaatat taatcagtct aaagtctcgc ggatgttgac caagtttggt
    85381 gctgtacgta cacgcaatgc caaaatggaa atggtttact gcctgccagc tgaactgggt
    85441 gtaccaacca cctccagtcc attgaagaat ctggtgctgg atatcgacta caacgatgca
    85501 gttgtcgtga ttcataccag ccctggcgcg gcgcagttaa ttgctcgcct gctggactca
    85561 ctgggcaaag cagaaggtat tctgggcacc atcgctggcg atgacaccat ctttaccacc
    85621 cctgctaacg gtttcaccgt caaagacctg tacgaagcga ttttagagct gttcgaccag
    85681 gagctttaat ctctgccccg tcgcttctgg cggcggggaa aatgttgctt atccctctca
    85741 accccctgct ttcccctgcg attaatttaa cgaatagtgc gttttactgc gacatgtcat
    85801 tcacataatg aatacatacg gtaaaaaaag cacattatgc aaaattcatt atctaattga
    85861 aaatactaga attaaaaata aatgaccgtg tttttaattc ttttttgtta ttaaaattca
    85921 catttttaac acttagtatc aactgaaaca gttagcgtgg tattaattag ttcaataatt
    85981 agtgtatgct tgattttgtg atatgggtca caaaacaaag gcccagctaa aagattatgt
    86041 cgaggtaaaa atcatgaaaa tcaaaaccac tgtagctgca ttaagcgtac tttctgttct
    86101 ctctttcggt gcattcgctg ccgactccat tgatgctgca caagcacaaa atcgtgaagc
    86161 aatcgggacc gtatccgtaa gtggtgtggc gtcttcgcca atggatatgc gtgaaatgct
    86221 gaacaaaaaa gcggaagaga aaggcgcaac ggcctaccag attactgaag ctcgtagcgg
    86281 tgacacctgg cacgctacgg ctgaactgta caaataaacc ctcatcgtct tgtccgacga
    86341 tattgccccc ggttcggggg ctttttttgc gctaatgacg aacattaaaa cgcaaatgcc
    86401 cttccagctc ttcctctgcc tcatcaaaca gcaatattaa cgcgccaaaa cggcgacgcg
    86461 ttttctctcc cagatgaaca aattcaatct caagtggtag cggcaggaca tcgttcatta
    86521 acacatccca tagtgagtcg agatcgcgta ccttatcttt cgccagacca aaggtttggc
    86581 taaagtcacg ataaaaatcc tcctgactct caatctcatc aaaatcaaag gtataaatat
    86641 tcatctattg ccaccgtcac gtttcgcggc gagttctgcc gtgcatcgct aagtatagcc
    86701 atgaaaaaac cgacactttt ggcgtcggtt ttgacctaac tatcggtcaa cgcatgttga
    86761 tacttatgga gcatacccgc cagccgatga accggttccg tcacctgcgg tggcgcttgc
    86821 cagatgcgta gcttttcctg gtagatttca agttcttcca gcaactggcc aaagtaccga
    86881 cgacgtttat catcgctacg ggcagatatc acatggtctg ctgtgcggcg catttgtcgg
    86941 tgaaacgccg ataaatcctc gttaaccggg atcggcgcat cacgcaggcg ctggtgcgcg
    87001 ataatcatcg tcagcgccag gcgaaatttc ggcaaatccc ctgggaactt attcatcagc
    87061 aaaaacagct gctgataaag tgccgggagg tggttctctt tacgacgtgc cacattggta
    87121 gtcatcgcgg aaacagcggc agaaacaaac tgattaagca gtacacgttc ggtcctgtcg
    87181 cgcgatttat cccgcacaag caaaataacg gtgaacgcga gcacacagcc gacgatttgc
    87241 cctaatgcgc tgtcgagaaa ctgactgaaa tggaaagtca tcgggttatc cagcacgata
    87301 atatttatgg tgctggccag cgcccccatc gagcccagtc gccgttgctg tacttctata
    87361 ccgaggaaaa atcccagcac tgccaggcta atacacagca gcaacatgct ctgttgggta
    87421 ttagggataa tcaccaaaaa gtagagcagc cctaacggca gcgcggccag cgtcccgtag
    87481 ataaagtcga tcgccaccat gcgtggattc ggcaaacgca ttgccagtga cgtcactacc
    87541 gcaatcatca ccattgcacc actgccggaa gtccagcccg tccacagcca gaaaagcgtg
    87601 cccagaatgc aggaaagtgt ggttcgccag aagttaacca tcgcatgatg acgttcggct
    87661 gactctactt tgacttccgg ttcgccttgc aggatctctt cttcggtggc gttgattttt
    87721 gtgttactga taacgccgcg cttgagaagc tgataacgcg ttgccgccgc gacccagcta
    87781 taaatggtga caggcgtttc ccgttccccg gtccaggcga taactctccg caggcgtttg
    87841 agctgcttgt ggacgtcctg cgcggtttct accggcgtgt caaaaaattc gcggaaagta
    87901 tcagtgatca attccgggcg cgtattctga ataagataag tttcgcagga ttgggtaatc
    87961 agcgtcagcg atagcgtatt gatcgctttt aaacgtcgat tggcccgcgc ccagcgggaa
    88021 gattccatat tcaggttgct gcgcatgcct tgtagcgccg tggtgcgtcg caccaggtcg
    88081 ccccaggctt tatcgacaac ttcaccatcg ccatgcttga tacagagttg cattaattga
    88141 tattgcgcga ccagcaaact ttccagctct cgatccactt cttgtttgat cgatcgcgga
    88201 gaaaagagca aatccgctat aatcgcacac acaataccga taacgatctc gctacaacgt
    88261 tcgacggcaa actgcggcgt aagcaatggt tccggctgaa tggtgatcac aatgatcagc
    88321 gcggtataac cggccagccc ccacgcgtac gagttttcta ttcgtaccag cgaggatatc
    88381 caggtacaaa aaccggccca gatacagcac accagaatca tcaataatgg tgcgcggatc
    88441 atcgcaatga tgatcaccag tccggcaata cagccaataa atgtgccgat gatgcgcaaa
    88501 aagccacgat aacgaatagc gccagaatac ggttcacctc ccgcagcaaa ggccggaccg
    88561 gcggcaacaa tcgccgctgt cagtaccgcc cagcgtggcg tttccagctg gaagtgaaag
    88621 ccaacaaaca gcgccagtac aatggcggtc gccagtttta ccgcaaagcg aatatgttgg
    88681 ttagcaatgg agaaaatacc catcgtgatt aaccaaactc acgcaggcga tgggccattt
    88741 tacggaagaa cgaatcctgg ctttcgtcgc gatcttgttt gccagtgacc accactgtag
    88801 cagtggtgcc cgcaggccag atgttctctt gctggttgtc gagacgaata cgaaccggaa
    88861 cacgttgcgc aagacgcacc cattcaaggt tagagtctat agtcgccatc cctttgtcgt
    88921 cacgcgtgct gctggcgttg gtgacccctg cggcaacact atcaacagtc cctttcagca
    88981 ctttgttact gccaagcggc gtgatctctg cacgataccc cggacgcacc ccttccagct
    89041 tagtttcttc catataggcc agtacataga aggagttctg tttcaccagc gcaaccgccg
    89101 ttgatcctcg agtaataaac tcaccggtat agacgttgag gttggtcacc cagccatctg
    89161 ctggcgcgcg gatcaccgtg cgttcaagat ccagttttgc cagatcgcgg gtcgcctgcg
    89221 ctttcgctaa ctgatgcaga accgtttgta gtacgttgtt agcctagtcg atctcttcgc
    89281 gagacatcgc ctgcacaccg agacggttac gacgtccggc ctcctggcgt ttctcctgtg
    89341 ccagtacctg ataataagca acatcggctt gcgcttcctc aagcgccttt tgatagcgcg
    89401 gctggtcgat ggtgaacagt atctgtcctt ttttcaccag ctggttatca tgaacattca
    89461 cctgggtaat gagtccagaa acgtccggcg cgatcgcaat gacgtcagcg ctaaagcgcg
    89521 cgtcacgcgt ccagggggat tcggtgtaat agacccaggc attaaaaatt gcgatgaagg
    89581 ccagaatgac taatacgacc gtgatggccg tacgggagaa ttttcttatt agtgttttca
    89641 cttcaacctc aaacgaacag tcgcgatatc aaataaaaca agcagcaata gagcgcggtg
    89701 ttgaacaacg ccggatgcca gacaaagtcg tagatacctg ttggcacaag tacccggcgc
    89761 accagccaga aaatcgccag tgataaaagc aattcaaaaa atatcggtgg gaaggacagc
    89821 ccaaacacca cgataacggg aaacagactc atgttgacct tggttgtata gagagagcag
    89881 gcgttattat tttcagcatc tgtcgccgca gagaagggca tggaaagccg ggcgagagca
    89941 acattgctgt agattgatat ttaatatatt agcgtaactg ttatgctgtt atcta
//
LOCUS       DAJTSL010000002        66348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTSL010000002 DAJTSL010000000
VERSION     DAJTSL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.002"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="OO433_000095"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="OO433_000095"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6048720.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQDRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO433_000096"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO433_000096"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6048721.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO433_000097"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO433_000097"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6048722.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIVGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO433_000098"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO433_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6048723.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO433_000099"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO433_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6048724.1"
                     /translation="MEFSPLLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO433_000100"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO433_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6048725.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALMQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="OO433_000101"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="OO433_000101"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6048726.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLEELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQSPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10127
                     /gene="mrcB"
                     /locus_tag="OO433_000102"
     CDS             7605..10127
                     /gene="mrcB"
                     /locus_tag="OO433_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6048727.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWAGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10347..12536
                     /gene="fhuA"
                     /locus_tag="OO433_000103"
     CDS             10347..12536
                     /gene="fhuA"
                     /locus_tag="OO433_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6048728.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12587..13384
                     /gene="fhuC"
                     /locus_tag="OO433_000104"
     CDS             12587..13384
                     /gene="fhuC"
                     /locus_tag="OO433_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6048729.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSSKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13384..14274
                     /gene="fhuD"
                     /locus_tag="OO433_000105"
     CDS             13384..14274
                     /gene="fhuD"
                     /locus_tag="OO433_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6048730.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSAEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14271..16253
                     /gene="fhuB"
                     /locus_tag="OO433_000106"
     CDS             14271..16253
                     /gene="fhuB"
                     /locus_tag="OO433_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6048731.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWMWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATSTMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16288..17568)
                     /gene="hemL"
                     /locus_tag="OO433_000107"
     CDS             complement(16288..17568)
                     /gene="hemL"
                     /locus_tag="OO433_000107"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6048732.1"
                     /translation="MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17793..19214
                     /gene="clcA"
                     /locus_tag="OO433_000108"
     CDS             17793..19214
                     /gene="clcA"
                     /locus_tag="OO433_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6048733.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVVVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSEILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19238..19303
                     /locus_tag="OO433_000109"
     CDS             19238..19303
                     /locus_tag="OO433_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6048734.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19296..19640
                     /gene="erpA"
                     /locus_tag="OO433_000110"
     CDS             19296..19640
                     /gene="erpA"
                     /locus_tag="OO433_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6048735.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19687..20310)
                     /locus_tag="OO433_000111"
     CDS             complement(19687..20310)
                     /locus_tag="OO433_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6048736.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20348..21147)
                     /gene="btuF"
                     /locus_tag="OO433_000112"
                     /pseudo
     CDS             complement(20348..21147)
                     /gene="btuF"
                     /locus_tag="OO433_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
     gene            complement(21140..21838)
                     /gene="mtnN"
                     /locus_tag="OO433_000113"
     CDS             complement(21140..21838)
                     /gene="mtnN"
                     /locus_tag="OO433_000113"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6048737.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFEADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21922..23439
                     /gene="dgt"
                     /locus_tag="OO433_000114"
     CDS             21922..23439
                     /gene="dgt"
                     /locus_tag="OO433_000114"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6048738.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRMKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23569..24993
                     /gene="degP"
                     /locus_tag="OO433_000115"
     CDS             23569..24993
                     /gene="degP"
                     /locus_tag="OO433_000115"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6048739.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25148..26305
                     /gene="cdaR"
                     /locus_tag="OO433_000116"
     CDS             25148..26305
                     /gene="cdaR"
                     /locus_tag="OO433_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR6048740.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGRLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIYRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26394..26780)
                     /locus_tag="OO433_000117"
     CDS             complement(26394..26780)
                     /locus_tag="OO433_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6048741.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26942..27754)
                     /gene="yaeI"
                     /locus_tag="OO433_000118"
     CDS             complement(26942..27754)
                     /gene="yaeI"
                     /locus_tag="OO433_000118"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR6048742.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27809..28633)
                     /gene="dapD"
                     /locus_tag="OO433_000119"
     CDS             complement(27809..28633)
                     /gene="dapD"
                     /locus_tag="OO433_000119"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6048743.1"
                     /translation="MQQLQNIIETAFERRAEITPTNADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGHVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28664..31336)
                     /gene="glnD"
                     /locus_tag="OO433_000120"
     CDS             complement(28664..31336)
                     /gene="glnD"
                     /locus_tag="OO433_000120"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6048744.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31398..32192)
                     /gene="map"
                     /locus_tag="OO433_000121"
     CDS             complement(31398..32192)
                     /gene="map"
                     /locus_tag="OO433_000121"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6048745.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32560..33285
                     /gene="rpsB"
                     /locus_tag="OO433_000122"
     CDS             32560..33285
                     /gene="rpsB"
                     /locus_tag="OO433_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6048746.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33543..34394
                     /gene="tsf"
                     /locus_tag="OO433_000123"
     CDS             33543..34394
                     /gene="tsf"
                     /locus_tag="OO433_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6048747.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34541..35266
                     /gene="pyrH"
                     /locus_tag="OO433_000124"
     CDS             34541..35266
                     /gene="pyrH"
                     /locus_tag="OO433_000124"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6048748.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35558..36115
                     /gene="frr"
                     /locus_tag="OO433_000125"
     CDS             35558..36115
                     /gene="frr"
                     /locus_tag="OO433_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6048749.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36207..37403
                     /gene="ispC"
                     /locus_tag="OO433_000126"
     CDS             36207..37403
                     /gene="ispC"
                     /locus_tag="OO433_000126"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6048750.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37591..38349
                     /gene="ispU"
                     /locus_tag="OO433_000127"
                     /gene_synonym="uppS"
     CDS             37591..38349
                     /gene="ispU"
                     /locus_tag="OO433_000127"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6048751.1"
                     /translation="MLSATQLLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFIDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38362..39219
                     /gene="cdsA"
                     /locus_tag="OO433_000128"
     CDS             38362..39219
                     /gene="cdsA"
                     /locus_tag="OO433_000128"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6048752.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVSLLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39231..40583
                     /gene="rseP"
                     /locus_tag="OO433_000129"
     CDS             39231..40583
                     /gene="rseP"
                     /locus_tag="OO433_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6048753.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GLQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGAHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40613..43045
                     /gene="bamA"
                     /locus_tag="OO433_000130"
     CDS             40613..43045
                     /gene="bamA"
                     /locus_tag="OO433_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6048754.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43167..43652
                     /gene="skp"
                     /locus_tag="OO433_000131"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43167..43652
                     /gene="skp"
                     /locus_tag="OO433_000131"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6048755.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43656..44681
                     /gene="lpxD"
                     /locus_tag="OO433_000132"
     CDS             43656..44681
                     /gene="lpxD"
                     /locus_tag="OO433_000132"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6048756.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44786..45241
                     /gene="fabZ"
                     /locus_tag="OO433_000133"
     CDS             44786..45241
                     /gene="fabZ"
                     /locus_tag="OO433_000133"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6048757.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45245..46033
                     /gene="lpxA"
                     /locus_tag="OO433_000134"
     CDS             45245..46033
                     /gene="lpxA"
                     /locus_tag="OO433_000134"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6048758.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVGIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            46033..47181
                     /gene="lpxB"
                     /locus_tag="OO433_000135"
     CDS             46033..47181
                     /gene="lpxB"
                     /locus_tag="OO433_000135"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6048759.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVARDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKISHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47178..47774
                     /gene="rnhB"
                     /locus_tag="OO433_000136"
     CDS             47178..47774
                     /gene="rnhB"
                     /locus_tag="OO433_000136"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6048760.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47811..51293
                     /gene="dnaE"
                     /locus_tag="OO433_000137"
     CDS             47811..51293
                     /gene="dnaE"
                     /locus_tag="OO433_000137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6048761.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGVNAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYASGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51306..52265
                     /gene="accA"
                     /locus_tag="OO433_000138"
     CDS             51306..52265
                     /gene="accA"
                     /locus_tag="OO433_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6048762.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52364..54473
                     /gene="ldcC"
                     /locus_tag="OO433_000139"
                     /pseudo
     CDS             52364..54473
                     /gene="ldcC"
                     /locus_tag="OO433_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
     gene            54530..54919
                     /locus_tag="OO433_000140"
     CDS             54530..54919
                     /locus_tag="OO433_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6048763.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54984..56282
                     /gene="tilS"
                     /locus_tag="OO433_000141"
     CDS             54984..56282
                     /gene="tilS"
                     /locus_tag="OO433_000141"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6048764.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKSAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56331..56591)
                     /gene="rof"
                     /locus_tag="OO433_000142"
     CDS             complement(56331..56591)
                     /gene="rof"
                     /locus_tag="OO433_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6048765.1"
                     /translation="MLMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56578..56778)
                     /locus_tag="OO433_000143"
     CDS             complement(56578..56778)
                     /locus_tag="OO433_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6048766.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56944..57489
                     /locus_tag="OO433_000144"
     CDS             56944..57489
                     /locus_tag="OO433_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6048767.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57486..57908
                     /gene="arfB"
                     /locus_tag="OO433_000145"
     CDS             57486..57908
                     /gene="arfB"
                     /locus_tag="OO433_000145"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6048768.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSAKAMRGKVRSGRE"
     gene            57922..58632
                     /gene="nlpE"
                     /locus_tag="OO433_000146"
                     /gene_synonym="cutF"
     CDS             57922..58632
                     /gene="nlpE"
                     /locus_tag="OO433_000146"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6048769.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58832..59652)
                     /locus_tag="OO433_000147"
                     /pseudo
     CDS             complement(58832..59652)
                     /locus_tag="OO433_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(59705..61423)
                     /gene="proS"
                     /locus_tag="OO433_000148"
     CDS             complement(59705..61423)
                     /gene="proS"
                     /locus_tag="OO433_000148"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6048770.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61534..62241)
                     /gene="tsaA"
                     /locus_tag="OO433_000149"
     CDS             complement(61534..62241)
                     /gene="tsaA"
                     /locus_tag="OO433_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6048771.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYHKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62238..62642)
                     /gene="rcsF"
                     /locus_tag="OO433_000150"
     CDS             complement(62238..62642)
                     /gene="rcsF"
                     /locus_tag="OO433_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6048772.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62760..63575)
                     /gene="metQ"
                     /locus_tag="OO433_000151"
     CDS             complement(62760..63575)
                     /gene="metQ"
                     /locus_tag="OO433_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6048773.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63615..64268)
                     /gene="metI"
                     /locus_tag="OO433_000152"
     CDS             complement(63615..64268)
                     /gene="metI"
                     /locus_tag="OO433_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6048774.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64261..65292)
                     /gene="metN"
                     /locus_tag="OO433_000153"
     CDS             complement(64261..65292)
                     /gene="metN"
                     /locus_tag="OO433_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6048775.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65480..66052
                     /gene="gmhB"
                     /locus_tag="OO433_000154"
     CDS             65480..66052
                     /gene="gmhB"
                     /locus_tag="OO433_000154"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6048776.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFVLVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15761 a        16436 c        18207 g        15944 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aattcgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gatcctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgagcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtggag
      481 tgcggtaaaa cgaagaaacg gcaagaatgt ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgcg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacggcgacg
      661 cggtgacggc tcttcatcca actcgttaag catccctttt tgatctggtg gcgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcgttacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc atgccgccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggttg caacttcgat aatctccggg ccaaacatca catgagccag
     1681 acggaaacgg cgacccacaa ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttattgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag cgtccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctacctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg agatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgccgcgc
     2521 agctggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgacaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaagataacc
     3841 gcacgctggc cttcagccgc tacgttcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttctgcg
     4021 ccatatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttagttc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc acgcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaagggga gagaattcca ttgcgacaat ccttgttatt
     4381 gcattagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acctggcggc gggattgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gcggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctcggcgct cacttcgcct aaaaatgcca gagtcagatg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccagtg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgttttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggaggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggccgaatac ccgcctggaa gtagttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggagtt gagcggtgtt ggattggtga
     5341 tccttgatga atttcatgag cgtagcttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctaat tatgtcggct acgctggaca
     5461 acgaccgctt gcagcaaatg ctgccagaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gatgccgttg
     5581 cggtagccac cgctgaaatg ctgcgtcagg aaagcggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgt gtgcaggaac aactggcttc gcgcatcggc agtgatgtat
     5701 tgctctgccc gctgtatggc gcgttgtcgc tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgt tttgatccgc
     5881 gcacggggct aacgcgactg attactcaac gcgttagcca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tctgcctgca tctaatcgcc aaagaacaag
     6001 cagaacgcgc tgccgcgcaa agtgaaccgg aaatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcag gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttagcgcg aagaacgacg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgtggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcgtcgccg tgggcaagat ggacgctatc aactggcaaa cggcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctgccggt cgatattgat gagttagtac
     6661 aacgctgccc gcagctggta cagcagtctg acactgtgga gtgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctgcaaatcg gtctgctgac ggtgaaagtg cagccgctgg
     6781 cgaaaccctc ggaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagttta ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgactg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaagagat gtttggcgag gccaccaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctggagtt gctttcacct gcccaaagtc
     7261 cattacaaat cacacgagat ttgagcgcct tctggaaagg agcgtaccgt gaggtgcaaa
     7321 aagagatgaa agggcgttat cccaaacatg tctggccgga cgacccggca aatactgcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgaacac aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cctgcggcag
     7921 tttatggccg aatggtcaat cttgagccag atatgaccat cagcaagaac gagatggtga
     7981 agctgctgga ggcgactcag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcat ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggctttttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcaa cgtctgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgcc atttctacga gcatgatggg atcagcctct
     8341 actcaattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgc tcttactggc
     8461 gtaaagcgaa cgaagcctac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgcggtg
     8821 gggtgatctc tcctcagcca gcctttatgc aactggtgcg tcaggagctg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtggg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 tggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacccgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcagt tacggatacc tggatcaaac tgggcgtacc gaaagatcag ctgcatccgg
     9421 ttccggcgat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcgttcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggcaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacttg gtttgcaggc attgacggca gcacggtgac catcacctgg gcaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcaac agcaacctgc tcagcaagag cagaaagaca
    10081 gcgacggtgt agccggttgg atcaaggata tgtttggtag taattaacat ctaagcgtga
    10141 aataccggat ggcgagttgc catccggtaa aataacatcc catctaagat attaaccctt
    10201 tcttttcatc tggttgttta ttaacccttc aggaacgctc agattgcgta ccgcttgcga
    10261 acccgccagc gtttcgaata ttatcttatc tttataataa tcattctcgt ttacgttatc
    10321 attcacttta catcagagat ataccaatgg cgcgttccaa aactgctcag ccaaaacact
    10381 cactgcgtaa aatcgcagtt gtagtagcca cagcggttag cggcatgtct gtttatgcac
    10441 aggcagcggt tgaaccgaaa gaagacacta tcaccgttac cgctgcacct gcgccgcaag
    10501 aaagcgcatg ggggcctgct gcaactattg cggcgaagca ctctgctact gcgactaaaa
    10561 cggatacacc aattgaaaaa acgccgcagt ctatttcggt tgtgacaaat gaagagatgc
    10621 agatgcatca atttcagtct gtaaaagaag cattaggtta tacacctggc gttacggtta
    10681 gcagtcgtgg tgcttctaat acatatgatt ttgttatcat tcgtggtttc tcatctgttg
    10741 gtctgaatca aaataattac cttgatggac taaaacttca gggcaacttt tataacgatg
    10801 ctgtgattga tccttacatg ctcgagaggg ttgaactgat gcgtgggccg acgtctgttc
    10861 tctatggaaa aagtaatcct ggtgggatta tttctatggt gagtaagcgt ccgaccactg
    10921 aacccctgaa agaaattcag tttaaaatgg ggacggataa tctgtttcag accggattcg
    10981 atttcagtga tgcactggat gataacggtg agttctctta ccgtttgacg ggcctcgctc
    11041 gctcaacaaa cgaacagcag aaaaactctg aatctcagcg ttataccatt gctccatcat
    11101 tctcatggcg tccagacgac aaaaccaatt ttactttcct gtcctatttc cagaatgaac
    11161 cagaaacggg ttattacggt tggttgccga aagaggggac cgttgagcca ttgcctaatg
    11221 gtaagcgtct accgactgac ttcaatgaag gtgcatcgaa taatacatac tcccgtaacc
    11281 agaaaatggt gggatatagt tttgaacatg gttttaatga caccttcacc gtgcgtcaga
    11341 atctgcgttt cagtgaaatg aaaacctcac agaaaagtgt ttatggcaca gggattgctg
    11401 gtgatggtca taccctaaac cgcgggacag tggtggataa tgagcgtcta caaaacttta
    11461 gcgttgatac ccaacttgaa agtaagtttg ctacaggtga cgtcgagcat actttgctga
    11521 caggggtaga cttcatgcgg atgcgtaatg atatcaatgc cagctttgga tccgcaccat
    11581 ccatcgatct ttataacaaa tatcaccctg aatactttgc atttggtagc gcagagccat
    11641 accagatgaa tgaaagcaaa caaacaggta tttatgttca ggatcaggcg gaatggaata
    11701 aatgggtatt cactctgggg ggacgctacg attggtctaa gcaagcgacc actgttcgtg
    11761 aaaactctta tacgccgact gaaggttata ttgagcgcaa tgatcatcag ttcacctggc
    11821 gcggtggtgt aaattactta ttcgataatg gtatttcacc ttactttagc tatagccagt
    11881 cctttgaacc gagtgctttc gatctgtgga gcaacccacg catttcctat aagccatcga
    11941 aaggtgaaca gtatgaagct ggcgtaaaat atgttccgaa tgatatgccg gtcgttgtta
    12001 cgggcgcagt ctatcaattg acgaaaacaa ataacctgac agcagaccca acaaacccat
    12061 tagcgcaagt cccagcaggt gagattcgcg ctcgtggtgt ggaacttgaa gcaaaagcgg
    12121 cgttaaatgc caatattaac ttgacggctt cttataccta caccgatgcg gaatacacca
    12181 aagacaccaa tctcaaaggt aaaactccag aacaagtacc ggagcatatg gcatctctct
    12241 ggggggatta taccttcaat gaagggccgc tttctggttt aacattggga acaggtggtc
    12301 gttttattgg ttccagctat ggtgatccgg caaacacttt taaagtgggt agcgcagctg
    12361 taatggatgc tgttgtaaaa tatgatctgg cacgctttgg tatggcggga tccagccttg
    12421 ctgtgaacgt caacaatttg ctcgatcgtg agtatgttgc cagttgcttc cagacttatg
    12481 gctgcttctg gggcgcagaa cgtcaggtcg ttgcaaccgc aaccttccgt ttctaatttc
    12541 tcttttgggg cacggatttc cgtgcccatt tcacaagttg gctgttatgc aggaatacac
    12601 gaatcattcc gataccactt ttgcactgcg taatatctcc tttcgtgtgc ccgggcgcac
    12661 gcttttgcat ccgctgtcgt taacctttcc tgccgggaaa gtgaccggtc tgattggtca
    12721 caacggttct agtaaatcca ctctgctcaa aatgcttggc cgtcatcagc ctccgtcaga
    12781 aggggagatt cttcttgatg cccagccgct ggaaagctgg agcagcaaag cgtttgcccg
    12841 caaagtggct tatttgccgc agcagcttcc tccggcagaa gggatgaccg tgcgtgaact
    12901 ggtggcgatt ggtcgttacc cgtggcatgg cgcgctgggg cgctttgggg cggcagatcg
    12961 cgaaaaagtc gaggaagcta tctcgctggt tggcttaaaa ccgctggcgc atcggctggt
    13021 tgatagtctc tctggcggcg aacgtcagcg ggcgtggatc gccatgctgg tggcgcagga
    13081 tagccgttgt ctgttgctcg acgaaccgac ctcggcgctg gatatcgccc accaggttga
    13141 tgtgctggcg ctggtgcacc gtttaagtca ggagcgtggc ctgacggtca ttgccgtgtt
    13201 gcacgatatc aatatggcgg cacgctactg tgattatctg gtcgccctgc gcggtggtga
    13261 aatgattgct cagggaacgc ctgcggaaat tatgcgcggc gataccctcg aaatgattta
    13321 tggcatcccg atgggtattt tgccgcatcc ggcgggtgct gcacctgtga gttttgttta
    13381 ttgatgagcg gcttacctct tatttcgcgc cgtcgactgt taacggcgat ggcgctttct
    13441 ccgttgttat ggcagatgaa taccgcccac gcggcggcta ttgatcccaa tcgtattgtg
    13501 gcgctggagt ggttgccggt ggaattactg ctggcgctcg gcatcgtgcc ttacggcgta
    13561 gcggatacca tcaactatcg cctgtgggtc agcgaaccac cattgccgga ctcagtgatc
    13621 gacgtcggtt tgcgcacaga acctaacctt gaactgctga ccgaaatgaa accgtcgttt
    13681 atggtctggt cggcaggata tggcccttca gcagaaatgc tggcacgtat tgcgccgggg
    13741 cgtggattta acttcagcga cggcaaacag ccgctggcga tggcgcgtaa atcactgacg
    13801 gaaatggcag atttacttaa cctgcaaagc gcagcagaaa cgcatttagc gcaatatgaa
    13861 gactttatcc gcagcatgaa accccgcttt gtgaagcgtg gtgcacgccc gttattgctg
    13921 acgacgctta tcgatccgcg ccatatgttg gtcttcggtc caaacagctt gttccaggaa
    13981 attcttgatg agtacggcat cccaaatgcc tggcaagggg aaaccaactt ctggggcagt
    14041 accgccgtca gtatcgatcg tctggcagcg tataaagacg ttgatgtgct ctgttttgat
    14101 cacgacaaca gcaaagacat ggatgcgcta atggcaaccc cgctgtggca ggccatgccg
    14161 tttgtccgcg ccggacgctt tcagcgcgta cccgcagtct ggttttatgg tgcgacgctc
    14221 tcggcaatgc actttgtgcg cgttctggat aacgccatcg gaggtaaagc gtgagtaaac
    14281 gaattgcgct tttcccggcg ttattgctgg cgctgctggt gattgtcgct acggcgctga
    14341 cctggatgaa cttttcgcag gcgctgccgc gtagccagtg ggcgcaggcc gcctggtcgc
    14401 cggatattga cgtcatcgag cagatgattt ttcactacag cttgctgccg cgtctggcga
    14461 tttcgctgct ggtgggcgca ggcctggggc tggtgggcgt gctgtttcag caagtgctgc
    14521 gtaatccgct ggcggaaccg acgacacttg gcgttgctac aggcgcgcaa ctggggatta
    14581 ccgtcactac gctctgggcg atcccagggg cgatggcgag tcagtttgct gcgctggcgg
    14641 gcgcttgcgt tgttggctta atcgtctttg gcgtcgcgtg ggggaaacgg ctttcgccgg
    14701 taacgctgat cctcgcgggg ctggtagtga gcctttattg tggggcaatc aatcagttac
    14761 tggttatctt ccaccatgac caactgcaaa gcatgttcct gtggagcacc ggaacgctga
    14821 cgcaaaccga ctggggcggt gttgagcgtt tatggccgca gttgctgggc ggtgtgatgc
    14881 tgacgttgct gctacttcgc ccgttaaccc tgatggggct tgatgatggc gtggcgcgca
    14941 atctcgggct ggccttgtcg ctcgcgcgtc tggcggcgct gtcgctggcg attgtcatca
    15001 gtgcgctgct ggtgaacgcg gtggggatta tcggctttat cgggttgttt gcgccgttgc
    15061 tggcaaaaat gctgggggcg cggcgtctgc tgccgcgact gatgctggcg tcgttgattg
    15121 gtgcgctgat cctctggctt tccgatcaaa tcatcctctg gctgactcgc gtgtggatgg
    15181 aagtgtccac cggttcggtc actgcgttga tcggtgcgcc gctgctactg tggctgttgc
    15241 cgcgtttacg cagcattagc gcgccggata tgaaggtcaa cgatcgtgtc gcggctgaac
    15301 gccaacatgt gctggcgttt gccctcgcgg gcggcgtgct gctgttgatg gctgtggtgg
    15361 tggcgctgtc gtttggtcgt gatgcgcacg gctggatgtg ggcgagcggg gcgttgctcg
    15421 atgatttaat gccctggcgc tggccgcgaa ttatggcggc gctatttgcg ggcgtcatgc
    15481 tggcggtggc gggctgtatt attcagcgac tgaccggcaa cccgatggca agcccggaag
    15541 tgctggggat tagctccggc gcagcgtttg gcgtggtact gatgctgttt ctggtaccgg
    15601 gtaatgcttt tggctggctg ttacccgcgg gaagtatcgg tgccgcggtg acgctgttga
    15661 ttattatgat cgccgccggg cgcggtggat tttccccaca ccgcatgtta ctggcgggga
    15721 tggcgttaag taccgcattc accatgcttt taatgatgtt gcaggcgagt ggtgacccgc
    15781 gaatggcgca agtgctcacc tggatctctg gttcgaccta caacgccacc gatgcgcagg
    15841 tctggcgtac cggaattgtg atggtgattt tgctggcgat taccccgctg taccgccgct
    15901 ggctgaccat tttacctctg ggcggtgata ccgcacgagc tgtgggaatg gcgctgacgc
    15961 cgacgcgaat tgcgctgctg ctgttagcgg cttgcctgac ggcgacctcg acgatgacta
    16021 ttggaccgtt gagttttgtt ggtttaatgg caccgcatat tgcgcggatg atgggttttc
    16081 gacggacgat gccacacatc gtaatttcgg cgctggtggg tggtttactg ctggtattcg
    16141 ctgactggtg tgggcggatg gtgctgtttc cattccagat cccggcgggg ctgctgtcga
    16201 cctttatcgg cgcgccatat tttatctatt tgttgagaaa gcagagccgt taattgagtg
    16261 cctcgcccgg tgggcgaggc aaaattatca caacttcgca aatacccgac gtgcagcatc
    16321 gatggtgtta ttgatatctt ccatgctgtg cgccacagac ataaagcccg cttcaaacgc
    16381 tgacggtgcc aggtaaacac cttcgtccag catcatatgg aagaaacgct taaagcgttc
    16441 cacgtcacag gccatcacat cctgatagca cgtcacggac tcggcgtcgg taaagaaaat
    16501 accgaacatg ccgccaacgt ggttaacgac cagcggagtt ccggcttctt ctgccgcttc
    16561 cagcagacct tctgccagac gtgttgtcag ctcatccagc gtttcgtgaa cgcccggctg
    16621 cgcgacttca ttcagacagg cgaaacccgc tgccatcgca atcgggttac cggaaagcgt
    16681 acccgcctga tagaccggac ccgtcggggc cagcgcatcc attacatcac gacgaccacc
    16741 gaatgcgcct accggcattc caccgccgat gattttgccg aggcaggtta aatctggcac
    16801 tacgccgtaa taatcctgtg cgccagccag tgccacgcgg aagccggtca tcacttcatc
    16861 gatgatcagc aacgcgccaa attcgtcgca cagcgcacgc agacctggca ggaactctgg
    16921 ctgcggcgga acacagttca tattgcctgc caccggctcg acgataatac aggcaatctc
    16981 ttgcgggtat tgctcaaacg cggcgcgtac agaagccaga tcgttataag tacaggttaa
    17041 ggtatgtttg gcgaaatctg ccggaacacc cggcgagttt ggctggccta acgtgagtgc
    17101 gccagaaccg gctttcacca gcaggcagtc agcgtgaccg tggtaacaac cttcaaattt
    17161 aatgattttg tcgcgaccgg taaaaccacg ggccaggcgg atggcgctca tggtcgcttc
    17221 agtgccggag ttcaccatgc gcaccatatc catggtcggg accagttcgg tcaccagttg
    17281 cgccattttc acttccattt cggtcggtgc accaaagctt aaaccacgct cggcggcttc
    17341 aatcacggca ttgcggatag ccggatggtt atggcccagc accatcggcc cccaggaacc
    17401 gacataatcg atataggctt tgccatcaac atcgtacaga taagcgccgt ccgctttttc
    17461 gataaacagt ggagtgccgc ccacgccagt aaaggcgcga acaggggagt tcacaccgcc
    17521 agggagcagc tcgcgcgctg cgctgtaaag attttcagac ttactcatgg agggttcctg
    17581 attcgtagaa aaagtgaatg gctgctattc tatgttattc ataacaagtt aaatactcgt
    17641 caaacatcag gctgcttgta ctggtgcaat cctgatttct tagagtataa aagttttgtg
    17701 catttgaaac attacgcttt gcaaaggatt ttcatggaac gtgcgagtaa aatgccgtca
    17761 tcttatttgt atgaccaata agtgatcatt ggatgaaaac tgatactccc tctttagaaa
    17821 caccgcaggc cgcgcgcctg cgacgcagac aactgattcg ccaacttctt gagcgcgata
    17881 aaaccccgtt agccattttg tttatggcgg cagtcgtcgg cacgcttgtc gggctggcag
    17941 cggttgcttt tgacaaaggt gtcgcctggt tgcagaacca acgtatgggg gcgctggtac
    18001 atactgctga taattatccg cttctgttaa ccgtcgcttt tctctgttcg gcggtgctgg
    18061 cgatgttcgg ctactttttg gtgcgcaaat acgcgccgga agcaggtggt tcggggatcc
    18121 cggaaattga aggggcgctg gaagatcaac gtcccgttcg ctggtggcgt gtgttgccgg
    18181 tgaaattctt tggcgggctg gggacactcg gcggaggcat ggtgctgggg cgcgaagggc
    18241 caaccgtgca gatcggcggc aatatcggtc gcatggtact cgatattttc cgcctgaaag
    18301 gtgatgaagc tcgccatacg ctgctggcaa ccggtgctgc tgcggggctg gctgcggcct
    18361 ttaacgcgcc gctggcgggt attttgttta ttatcgaaga gatgcgtccg cagtttcgct
    18421 atacgttaat ttcgattaaa gcggtattta ttggcgtcat tatgtcgacc attatgtacc
    18481 ggatttttaa tcatgaagtt gcgttgattg acgtcggtaa actttctgat gcgccgctta
    18541 atacgctgtg gctttatctg atcctcggta ttatttttgg cattttcggc cctattttta
    18601 ataaatgggt gctggggatg caggatttgc tgcaccgtgt gcacggcggc aatattacca
    18661 aatgggtgct aatgggcggt gcgattggcg gtctgtgtgg attgctgggg tttgtggcac
    18721 cagcaacgtc gggcggcggt tttaacctga ttcctatcgc taccgcaggg aatttcagca
    18781 tgggaatgct ggtgtttatc ttcgtcgcgc gggtcattac caccttactg tgcttctctt
    18841 ccggcgcgcc gggcggtatt tttgccccga tgctggcgct gggtactgtg ctgggaaccg
    18901 ctttcggaat ggttgtcgtt gagctgtttc cgcaatatca ccttgaggcg gggacttttg
    18961 ctattgccgg aatgggggcg ttactggcgg catctattcg cgcgccgtta acggggatca
    19021 ttttggttct ggagatgacc gataactacc agctcatttt gccaatgatt attaccggtc
    19081 ttggcgcaac actattagcg caatttaccg gcgggaaacc gctatattcg gagattcttg
    19141 cgcgcacgct ggcaaaacag gaagctgagc aactggcgcg aagcaaggcc gcatcagcca
    19201 gcgagaatac ttgaacgaaa taccagggta ttagataatg gcgattatta ttgggttaga
    19261 atttgcccaa ttgccgatgt cgtttggagc aaaatatgag tgatgacgta gcactgccgc
    19321 tggagtttac cgacgcagca gccaacaaag ttaaaagcct gatcgctgac gaagataacc
    19381 cgaatctgaa attacgcgtg tatatcaccg gtggcggttg cagcggcttc cagtatggtt
    19441 tcacctttga tgatcaggtg aacgaaggcg atatgaccat cgaaaaacag ggcgttggcc
    19501 tggtggttga tccgatgagc ctgcaatatc tggtcggtgg ttccgttgac tataccgaag
    19561 gactggaagg ttctcgtttc atcgtgacca acccgaacgc gaaaagcacc tgcggttgcg
    19621 gttcttcctt tagcatctaa tctgttgttc atgtgccgga taaactatcc ggcacatctt
    19681 ctctcttcaa cgcccattct catccagcgc aaacgtcggt agcttaagat gccaacgaat
    19741 agccgccagc cgaatcaata gcgtcacgac catgcccatc atactggctg tttccagtgg
    19801 tacggaaaat gtgtaataag ccgtagcgtg gacaataccg ccgataatac aggcagttgc
    19861 gtagatttct gtacgtaaaa tcatggggat ttcgcgggcc agtacatcac gaatgatccc
    19921 gccgccaacg ccagtaatga cgcccataca aaccgcgatt aacggaccgg cttccgcatt
    19981 aaaggcttta ttcacgccaa tgccgacaaa caccgccaga ccaacggcgt ccagcaccgg
    20041 caacatccat tttggtaagc gtcttggctg gcgcaccagc acgatggtca gcatgctggt
    20101 taccattgca acgaccagat cggtgggatc tttcacccaa aataccgggc cgtgatccag
    20161 cgccatgtcg cgaattgtcc cgccgcctac agcggtaacc acgcccagta ccagaacacc
    20221 aaaagggtcc atacgcaatt ttccggctaa caaaacgccg gagatggcaa ataccgctgt
    20281 gccgactata tccagccaat agacgagcat tgttcaatcc ccactgagca cctaaaaagg
    20341 tcaggcgcta atctacctgt gaaagcgcat tacagagctg ttgtgcagcg aggataatac
    20401 gtgggcttgc acgtcaaacc agtcactcgt gagaggaata acgggaattt tgagctgttc
    20461 accccagtat tgtttgattt taggaatttg gtccggtccg cctgtaatga caatcgcctg
    20521 tggcgagcgt gctaacacct gttcgcggct aacttgcggc caaggaaccc ggctgtcttt
    20581 aaagatgttt tctccgccac aaacttcgag tacctggttt tgaatcgact cttttccact
    20641 ggtaaatggc ggattaatgc cgaattgcag aaaaacacgt tttttaggtt tatcagcata
    20701 ttgcgctttc aattgcgcgt actgatccag cagggattgc gcggcttgtt cggccttatc
    20761 cggttgcgga ctccaggggg ccagttgacg taacgcattg gcaatttgtt caatgcttgt
    20821 cgcatcgacc cacatcactt ttattcccgg cgaagccagc tggtcaacct gccgctcggc
    20881 attacctcca cgccaggcaa tcaccagatc gggtttcagc gcgacaatgc gttccagatt
    20941 catcccctgc caggtggaaa cctgctcaat cttttgcgct tgtggaggat agtcggaata
    21001 gctgctgacc ccaaccggcg tgatcccggc ggcaaaggca agttcagtgt tggcgggaga
    21061 aagcgtgatg acgcgcggtg ccgcgttgag ccacagtggc gcgagaaaag acagggcgac
    21121 cagcgccctg aacagtgact tagccatgtg ccagtttctg caccagtgac tcaaccatca
    21181 ggctggactg tttagcggca acagccagga actcatcgaa gctaagatga gactgttgat
    21241 cggccacgtc ggagatggcg cgtacgacaa caaacgggac gttgaaattg tggcagacat
    21301 gggcgattgc cgtcgcttcc atctctacag caatggcctg tgggaagttg tggcggattt
    21361 tcgccagacc aacagaaccg ttgatgaaag cgtcgccgct aacaatcagg ccacgtacag
    21421 cgttaagatt cagttcggca atgcaggcct cagcggcagc gatcagttta tcgtcagctt
    21481 caaagcctgc cggacagcct ggtaactgac cgtattcata accaaatgcc gtgacatccg
    21541 cgtcgtgata acgtgcttcg tccgagacaa cgatatcgcc cactttcaac gttggtgcca
    21601 ggccaccggc agaaccggtg ttaataatca catctggctt gcagtgttcc aacagcaaag
    21661 tggcacccag cgccgcagcg actttaccga tgcccgattt cagaagcgca acctcggttc
    21721 cattcagttg gccggtatag atttcgcaac cgccgagact gatagtttga cgattttcga
    21781 ttttgtcacg cagcagcgta acttcttctt ccattgcacc aatgatgccg attttcatag
    21841 atttactcgc gataagcccg atttgaaggc atagtttacc atgcgcttac ggggaagcgt
    21901 atttctcacg cgggagagga catggcacag attgatttcc gaaaaaaaat aaactggcat
    21961 cgtcgttacc gttcaccgca gggcgttaaa accgaacatg agatcctgcg gatcttcgag
    22021 agcgatcgcg ggcgtatcat caactctccg gcaattcgtc gtctgcaaca aaagacccag
    22081 gtttttccac tggagcgcaa tgccgccgtg cgcacgcgtc ttacccactc gatggaagtc
    22141 cagcaggtgg ggcgctacat cgccaaagaa attctaagcc gtatgaaaga gcttaaatta
    22201 ctggaagcat acggcctgga tgaactgacc ggcccctttg aaagcattgt tgagatgtca
    22261 tgcctgatgc acgatatcgg caatccgccg tttggtcatt ttggcgaagc ggcgataaat
    22321 gattggtttc gccagcgttt gcacccggaa gatgccgaaa gccagcctct gactgacgat
    22381 cgctgcagcg tggcggcact acgtttacgg gacggggaag aaccgcttaa cgagctgcgg
    22441 cgcaagattc gtcaggactt atgtcatttt gaggggaatg cacaaggcat tcgtctggtg
    22501 catacattga tgaggatgaa tctcacctgg gcacaggttg gcggtatttt aaaatatacc
    22561 cgtccggcgt ggtggcgtgg cgaaacgcct gagacacatc actatttaat gaaaaagccg
    22621 ggttattatc tttctgaaga agcctatatt gcccggttgc gtaaagaact taatttggcg
    22681 ctttacagtc gttttccatt aacgtggatt atggaagcag ccgacgacat ctcctattgt
    22741 gtggcagacc ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat
    22801 catttgcacg aagcgtgggg ccagcatgag aaaggttcgc tcttttcgct ggtggttgaa
    22861 aatgcctggg aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt
    22921 atgtatttac gggtaaacac cctaaataaa ctggtaccct atgcggcaca acgatttatt
    22981 gataatctgc ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc
    23041 gaatgcagcg atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat
    23101 ccagatgtcg agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt
    23161 tatcgtcctt tattaagcct gtcgttatcc gactttactg aactggtaga aaaagaacgg
    23221 gtgaaacgtt tccctattga atcgcgctta ttccacaaac tctcgacgcg ccatcggctg
    23281 gcctatgtcg aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa
    23341 tattattacc gttgccgcct gctgcaggat tatatcagcg gtatgaccga cctctatgcg
    23401 tgggatgaat accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa
    23461 caataaattt ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc
    23521 tgaagaacac agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc
    23581 acattagcac tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca
    23641 acggcggctg agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg
    23701 ctcgaaaagg tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat
    23761 acgccgcgta tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag
    23821 gaaggttctc cgttccagag ctctccgttc tgccaggggg gccagggcgg taatggtggc
    23881 ggccagcaac agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc
    23941 tatgtcgtca ccaacaacca cgttgttgat aacgcgacgg tgattaaagt ccagctgagc
    24001 gatggccgta agttcgacgc gaagatggtc ggcaaagatc cgcgctctga tatcgcgctg
    24061 atccaaatcc agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg
    24121 cgcgtgggtg attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact
    24181 tccgggattg tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc
    24241 atccagaccg atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac
    24301 ggcgaactga tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc
    24361 ggttttgcaa tcccgagcaa catggtgaaa aacctgacct cgcagatggt ggaatacggc
    24421 caggtgaaac gcggtgagct gggtattatg gggactgagc tgaactccga actggcgaaa
    24481 gcgatgaaag ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc
    24541 gctgcaaaag cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc
    24601 agcagctttg ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc
    24661 ctgggcttac tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc
    24721 cagaatcagg ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac
    24781 aaaggcaaag atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag
    24841 atcggcctga agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc
    24901 gctgaactgc gtaaagttct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc
    24961 ggcgacagca ccatctacct gttaatgcag taatctcact caaccccttc ctgaaaacgg
    25021 gaaggggttc tccttacaat ctgtgaactt caccacaact ccatacatct tcatcatcct
    25081 ttaggcattt gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg
    25141 aggacttatg gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac
    25201 catgcgcatc atcgatacca atatcaacgt aatggatgcc cgtgggcgaa ttatcggcag
    25261 cggcgatcgt gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg
    25321 acgagtcgtc gatatcgatg acgcggtggc acgtcatctg cacggtgtgc ggcaagggat
    25381 taatctaccg ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc
    25441 agagaatctg cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca
    25501 gtcgcggttg atgcacttgt tggcgcagga tagccgtttg cgggaggaac tggtgatgaa
    25561 cctgattcag gcagaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat
    25621 cgatctcaat caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggtgt
    25681 ggacagcgca atggcggagt tacaacaact gcaaaacgcg ctgactacgc ccgagcgtaa
    25741 taatctggtg gcgattgtct cgctaaccga aatggtggtg ttgaaaccgg cgttgaactc
    25801 ttttgggcgc tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat
    25861 gaaagagtac ggccggctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg
    25921 cagtattgcc cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat
    25981 gccagaaagt cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt
    26041 gcgtggcgac tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga
    26101 caataacggc ttgctgcgac gaacgctggc ggcgtggttt cgtcacaatg tgcaaccgct
    26161 ggcaacgtca aaggcgttgt ttatttatcg taataccctg gagtatcggc ttaatcgtat
    26221 atcggaactg accgggcttg atttgggcaa ttttgatgac aggttgctgc tgtatgtggc
    26281 gttacaactg gatgaagagc ggtaggttat gcgttaaggt tgaggcggcg ctgtctcatt
    26341 cccctcagcc cgaaggggag aggggaatgc ccgaattaac cgtcaattcg ggattattta
    26401 cgcgttagtt tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc
    26461 aggtgacgta agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg
    26521 cagatttgat caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct
    26581 ttataaccct gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac
    26641 ttaacgctct tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg
    26701 ttgttggctt cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga
    26761 cttttcagat tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt
    26821 agcatttggc ctagcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac
    26881 ttctatcatg gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt
    26941 tctacaccag ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac
    27001 tgcccacgcc acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt
    27061 aacgtttatc ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc
    27121 cgtgagtatg gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt
    27181 cgggattatg tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc
    27241 attgtccggc ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga
    27301 tcaccgtagc ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta
    27361 agtgattttt ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg
    27421 cacattcggc aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa
    27481 ataatacgta atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa
    27541 tcgcgtcaga aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa
    27601 gaattttgaa tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa
    27661 accagcctgg ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg
    27721 cagtagcctg caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg
    27781 cagcgtcgtg ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc
    27841 cgactttgcc gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt
    27901 atttgccatc ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgtgac
    27961 cgtagtggat ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc
    28021 ccatggaaat gacggaacct tcttcgacaa tcaccccttc aactacttca gagcgtgcgc
    28081 caatgaagca gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc
    28141 cgatgcccac gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg
    28201 tcgcccaggt atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg
    28261 gcatcagcac ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa
    28321 cgcggaagcc ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc attggcactt
    28381 tgtcgaagta gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga
    28441 gcagcaccgc ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta
    28501 cacgcagtgc accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg
    28561 tgtctgcgtt ggttggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct
    28621 gtaactgctg cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg
    28681 gattgagggc ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagcgcac
    28741 gccggtcagc ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc
    28801 gggcaccatg aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc
    28861 ctggctggtc gagcgcgata agctcaagga acgatttgcg gtcggtatgg gtcggcaaaa
    28921 acgttacttc ggtttcaaca gtaaaatggc gtaatttggc gggttggcga cggggctgcg
    28981 gtggctgcca gctactttgc gtcagtactt gctccagacc aaaccgaata acctcatgac
    29041 gatctgcgga cagcgggctg ccatcgggtt ccagcacgat aaaggtatcc atcgccatac
    29101 cgtcgcgagt ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg
    29161 cacagacggc ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc
    29221 cacgcgtagc ctgcgggcta agcaatacca gtggtttgct taaatcatgc tgtaataaat
    29281 ggcgggcatg ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc
    29341 gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg
    29401 cctggagttg gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc
    29461 gtagctgctt ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc
    29521 acagcgtttc gttggtggcg caaatgtcag ccactgtcag gcataccaga tagcgcagac
    29581 gattttccgt ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc
    29641 ggcgttgtgc tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg
    29701 tttcgcgtga gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa
    29761 tggagtggtc gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca
    29821 gctcagttga cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt
    29881 cttcactggc aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt
    29941 aggcgtggaa cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg
    30001 cgccgagcac gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac
    30061 gcagaatgct caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat
    30121 ggcgacgggc atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt
    30181 tgcgcaccat ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt
    30241 catcacgcag gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt
    30301 tttcgtcggc aggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga
    30361 gttcactgac gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt
    30421 tgccttcacc actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc
    30481 gattatcgta acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca
    30541 gacattcgtt taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat
    30601 ccagcgatgt tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca
    30661 agccgccagg gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct
    30721 gatggcgctg gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc
    30781 cttcgctaaa aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc
    30841 gggattcgat taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt
    30901 caagcgtgcg cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct
    30961 cgcccacttt ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga
    31021 tgtcagaaag tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt
    31081 cggcaatctg gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact
    31141 cggtgcgcgc ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc
    31201 aacgctggaa agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc
    31261 agacgcatgg attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt
    31321 ctggaagggt attcattgtg cgccactcat aagattaaat tatcgcatta aaaaagccgg
    31381 cattcgccgg cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg
    31441 cgtagcgtca gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca
    31501 gacaagctgc gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt
    31561 ttacccgcgt tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg
    31621 gtttcacggg agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg
    31681 tgtccgcaat attcacgaac gacggagaag ccttctgctt cgacaaactt ctgaatcgcc
    31741 gcaccgattt cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt
    31801 tcttgcgtga tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt
    31861 ttcgaggtat cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg
    31921 ccatctttca gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag
    31981 atgcaaacgg atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca
    32041 ttaacaatgt aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca
    32101 tacggttcga tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg
    32161 atatcttctg gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac
    32221 cgacaatata tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc
    32281 tgaatttcgc caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc
    32341 tgtacacagc gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg
    32401 acttccgatc catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac
    32461 aacacacacg tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg
    32521 tggaggcata accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc
    32581 gacatgctca aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg
    32641 aagccgttca tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta
    32701 ccgatgttca acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc
    32761 cttttcgttg gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc
    32821 gaccagttct tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt
    32881 cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa
    32941 aagctgacca agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc
    33001 ctgggcggta tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac
    33061 cacgaacaca ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt
    33121 gataccaact ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc
    33181 cgtgctgtga ccctgtacct gggcgctgta gctgcaaccg tacgtgaagg ccgttctcag
    33241 gatctggctt cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact
    33301 cccccaaaat agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca
    33361 tcagtaagtg accgggatga gcgagcgaag ataacgcatc tgcggcgcga aatatgaagg
    33421 gggagagccc ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc
    33481 tttttcactt ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta
    33541 gaatggctga aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca
    33601 tgatggattg caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa
    33661 acatgcgtaa gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg
    33721 gcgtgatcaa aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga
    33781 ctgacttcgt tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag
    33841 ctgttgctgg caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg
    33901 ttgcgctggt agcgaaaatt ggtgaaaaca tcaacattcg ccgcgttgct gcgctggaag
    33961 gcgacgttct gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag
    34021 gcgctgacga agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat
    34081 tcatcaaacc agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg
    34141 atatagcgat gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca
    34201 tgaagaaatt caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca
    34261 aaactgttgg tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg
    34321 aagtgggtga aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt
    34381 ccaagcagtc ttaattatca aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc
    34441 ttgtaaattc agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt
    34501 attaattcat ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc
    34561 cgtctataaa cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt
    34621 cggtattgat gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact
    34681 gggtattcag gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc
    34741 gaaagcgggt atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa
    34801 cggcctggca atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc
    34861 tattccattg aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg
    34921 caacaaccgt gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc
    34981 agcagcttgc ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt
    35041 tgacggcgtg tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact
    35101 gacttacagc gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct
    35161 ggctcgtgac cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg
    35221 ccgtgtggta atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat
    35281 aaataagggt aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta
    35341 ttgttgcagg cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg
    35401 aggatggcga gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg
    35461 attacccgta atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt
    35521 ctggtctgac tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga
    35581 tgctgaagta cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg
    35641 cacgggtcgt gcttctccca gcctgctgga tggcattgtc gtagaatatt acggcacgcc
    35701 gacgccgctg cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa
    35761 cgtgtttgat cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg
    35821 cctgaacccg aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga
    35881 acgtcgtaaa gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt
    35941 acgtaacgtg cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat
    36001 cagcgaagac gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat
    36061 caagaaaatt gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct
    36121 tgaacgacaa aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt
    36181 tattctgtct caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct
    36241 cgattggttg cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg
    36301 cgctggtggc aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc
    36361 gctatgccgt aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac
    36421 agggtagccg caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg
    36481 aggatgttga tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg
    36541 ctgcgatccg cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg
    36601 gacgtctgtt tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg
    36661 aacataacgc catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg
    36721 accttgagca aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg
    36781 agacgccatt gcgcgatttg gcaacaatga cgccggatca agcctgccgt catccgaact
    36841 ggtcgatggg gcgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat
    36901 acattgaagc gcgttggcta tttaacgcca gcgccagcca gatggaagtg ctgattcacc
    36961 cgcagtcagt gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg
    37021 gggaaccgga tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact
    37081 ctggcgtgaa gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt
    37141 atgatcgtta tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga
    37201 cgacagcatt gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc
    37261 gctttacgga tatcgctgcg ttgaatttat cggtactgga aaaaatggat atgcgcgaac
    37321 cacaatgtgt ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag
    37381 aggtgatgcg tctcgcaagc tgaggataat ccggctacag agagtctcgc tatttgttag
    37441 cgtagggctt cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt
    37501 caggtatgcc gtggttttgc acggcttttt ttgtataggc ttcagtattc ctgagtaccg
    37561 taaaccctgt cagggaataa aaaacgcgtg atgttgtctg ctactcaact acttagcgaa
    37621 aaattgccag cgcatggctg ccgtcatgtt gcgatcatta tggacggcaa tggtcgctgg
    37681 gcaaaaaagc aagggaagat tcgtgccttt gggcataaag ccggggcaaa atccgtccgc
    37741 cgggctgtct cttttgcggc caacaacggt attgaggcgt taacgctgta tgcctttagt
    37801 agtgaaaact ggaaccgacc agcgcaggaa gtcagtgcgt taatggaact gtttgtgtgg
    37861 gcgctcgata gcgaagtaaa aagtctgcac cgacataacg tgcgtctgcg tattattggc
    37921 gataccagtc gctttaactc gcgtttgcaa gaatgtattc gtaaatctga agcgctaaca
    37981 gccgggaata ccggtctgac gctgaatatt gcggcgaact acggtggacg ttgggatata
    38041 gtccagggag tcaggcaact ggctgaaaag gtgcagcaag gaaacctgca accagatcag
    38101 atagatgaag agatgctaaa ccagcatgtc tgtatgcatg aactggcccc tgtagattta
    38161 gtaattagga ctggggggga gcatcgcatt agtaactttt tgctttggca aattgcctat
    38221 gccgaacttt actttataga tgttctctgg cccgatttcg atgaacaaga ctttgaaggg
    38281 gcgttaaatg cctttgctaa tcgagagcgt cgtttcggcg gcaccgagcc cggtgatgaa
    38341 acagcctgat ggggggcgct tttgctgaag tatcgcctga tatctgcttt tgtgttaata
    38401 cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg ggttcgccat tgtaacgctg
    38461 gtggtctgca tgctggcagc gtgggaatgg ggacagctta gcggttttac cactcgttcg
    38521 cagcgagtat ggttggcggt gttatgcggg ttattgttgg cgctgatgct ttttctgttg
    38581 ccggaatatc accgaaatat tcatcaaccg ctggttgaaa tctcactttg ggcttcgctg
    38641 ggttggtgga ttgtctcgct attgctggtg ctgttttacc caggttccgc agcaatctgg
    38701 cgtaactcta aaacattgcg ccttattttt ggcgtgctaa ccattgttcc cttcttctgg
    38761 ggcatgctgg cgttacgggc ctggcactat gacgagaatc attacagtgg cgcaatatgg
    38821 ctgctctatg tcatgatcct ggtatggggc gctgactccg gcgcatatat gtttggcaaa
    38881 ttgtttggta aacataagct ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt
    38941 ataggtggac tcgctactgc agcggtaatc tcatggggtt atggcatgtg ggcgaatctc
    39001 gacgtcgctc ccgtcacctt actcatttgc tctattgtcg cagcgttagc ctcagtgctc
    39061 ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa ttaaggacag cggtcattta
    39121 attccaggac acggtggtat tttagatcgt attgatagcc tgacggctgc ggtaccggtc
    39181 tttgcttgct tgttgttact ggtattcagg acgctttaac ggaaggtaat atgctgagtt
    39241 ttctctggga tttggcttcg ttcatcgttg cactgggtgt acttatcact gtgcatgaat
    39301 ttggtcattt ctgggttgcc cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt
    39361 ttggtaaggc gctctggcgg cgaactgata agctcggcac cgaatatgtt atcgccctga
    39421 tcccgctggg cggttatgtc aaaatgctgg atgagcgcgc agaaccggtc gttccggaac
    39481 tccgccacca tgccttcaat aataaatctg ttggccaacg agcggcgatt attgccgcag
    39541 gtccggttgc aaacttcatt tttgctatct ttgcctactg gctggttttt attattggtg
    39601 tgcctggcgt acgtccggtg gttggtgaaa tagcagccaa ttcgatagct gcggaagcac
    39661 aaattgcacc aggtacggaa ctaaaagccg tagatggtat cgaaacgcct gattgggatg
    39721 ccgtgcgttt gcagttggtc gataaaattg gcgatgaaag caccaccatt acagtagcgc
    39781 catttggcag cgaccaacgg cgggatgtaa agctcgattt acgtcactgg gcgtttgagc
    39841 ctgataaaga agatccggta tcttcgctgg ggattcgtcc tcgtgggctg caaattgaac
    39901 ctgtactgga aaatgtgcag ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg
    39961 acaggatcgt taaagtcgat ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg
    40021 tccgggataa cccggggaaa tccttagcgt tagaaatcga aaggcaggga agtcccttgt
    40081 ctttgacatt aatcccggag agtaaaccgg gtaatggtaa agcgattggt tttgtcggta
    40141 ttgagccgaa agtcattcct ttgccagatg agtataaagt tgtacgccag tatgggccgt
    40201 tcaacgccat cgtcgaagcc acggacaaaa cgtggcagct gatgaagctg acggtcagta
    40261 tgctgggaaa attgatcacc ggtgatgtga aactgaacaa cctcagtggg ccgatctcta
    40321 tcgccaaggg ggctgggatg acagcggaac tcggggtagt ttattacctg ccgtttcttg
    40381 cgcttattag cgtgaactta gggataatta acctgtttcc gttgcccgta cttgacgggg
    40441 cgcatctgct gttccttgcg atcgaaaaga tcaagggcgg accggtatcc gagcgggttc
    40501 aagacttttg ttatcgcatt ggctcgattc tgctggtgct gttaatgggg cttgcacttt
    40561 tcaatgattt ctctcggtta tgagagttag ttaggaagaa cgcataataa cgatggcgat
    40621 gaaaaagttg ctcatagcgt cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg
    40681 gttcgtagtg aaagatattc atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct
    40741 cctcagtatg ccggtgcgta caggcgacac ggttaatgat gaagatatca gtaataccat
    40801 tcgcgctctg tttgctaccg gcaactttga ggatgttcgc gtccttcgtg atggtgatac
    40861 ccttctggtt caggtaaaag aacgtccgac cattgccagc attactttct ccggtaacaa
    40921 atcggtgaaa gatgacatgc tgaagcaaaa cctcgaggct tctggtgtgc gtgtgggcga
    40981 atccctcgat cgcaccacca ttgccgatat cgagaaaggt ctggaagact tctactacag
    41041 cgtcggtaaa tatagcgcca gcgtaaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt
    41101 tgacctaaaa ctggtgttcc aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt
    41161 tggtaaccat gctttcacca ccgacgaact gatctctcat ttccaactgc gtgacgaagt
    41221 gccgtggtgg aacgtggtag gcgatcgtaa ataccagaaa cagaaactgg cgggcgacct
    41281 tgaaaccctg cgcagctact atctggatcg cggttatgcc cgtttcaaca tcgactctac
    41341 ccaggtcagt ctgacgccag ataaaaaagg tatttacgtc acggtgaaca ttaccgaagg
    41401 cgatcagtac aagctttctg gcgttgaagt gagcggcaac cttgccgggc actccgctga
    41461 aattgagcag ctgactaaga tcgagccggg cgagctgtat aacggcacca aagtgaccaa
    41521 gatggaagat gacatcaaaa agcttctcgg tcgctatggt tatgcctatc cgcgcgtaca
    41581 gtcgatgcct gaaattaacg atgccgacaa aaccgttaaa ttacgcgtga acgttgatgc
    41641 gggtaaccgt ttctacgtgc gtaagatccg ttttgaaggt aacgatacct cgaaagatgc
    41701 cgtcctgcgt cgcgaaatgc gtcagatgga aggtgcatgg ctgggaagcg atctggtcga
    41761 tcagggtaag gagcgtctga atcgtctggg cttctttgaa actgtcgata ccgataccca
    41821 acgtgttccg ggtagcccgg accaagtaga tgtagtctac aaggtgaaag aacgtaacac
    41881 cggtagcttc aacttcggga ttggttacgg tactgaaagt ggcgtgagct tccaggctgg
    41941 cgtgcaacag gataactggt taggtacagg ttatgctgtt ggtatcaacg ggaccaaaaa
    42001 cgattaccag acctatgctg aattgtcggt aaccaacccg tacttcactg tagatggtgt
    42061 aagcctcggt ggtcgtctct tctataatga cttccaggca gatgacgccg acctgtccga
    42121 ctataccaac aagagttatg gtacagacgt gacgttgggc ttcccgatca acgaatataa
    42181 ctcgctgcgt gcaggtctgg gttatgtaca taactccctg tccaacatgc agcctcaggt
    42241 tgcgatgtgg cgttatctgt actctatggg tgaacatccg agcacctctg atcaggataa
    42301 cagcttcaaa acggacgact tcacgttcaa ctatggttgg acctataaca agcttgaccg
    42361 tggttacttc ccgacagatg gttcacgtgt caacctgacc ggtaaagtga ccattcctgg
    42421 atcggataac gaatactaca aagtgacgtt agacacggcg acttatgtgc cgatcgatga
    42481 cgatcacaaa tgggttgttc tggggcgtac ccgctggggt tatggtgatg gtttaggcgg
    42541 caaagagatg ccgttctacg agaacttcta tgccggtggt tccagcaccg tccgtggctt
    42601 ccagtccaat accattggtc cgaaagcagt ttacttcccg catcaggcca gtaattatga
    42661 tccggactat gattacgaat gtgcgactca ggacggcgcg aaagacctgt gtaaatcgga
    42721 tgatgctgta ggcggtaacg ccatggcggt tgccagcctc gagttcatca ccccgacgcc
    42781 gtttattagc gataagtatg ctaactcggt tcgtacttcc ttcttctggg atatgggtac
    42841 cgtttgggat acaaactggg attccagcca atattctgga tatccggact atagtgatcc
    42901 aagcaatatc cgtatgtctg cgggtatcgc attacaatgg atgtccccat tggggccgtt
    42961 ggtgttctcc tacgcccagc cgttcaaaaa gtacgatgga gacaaggcag aacagttcca
    43021 gtttaacatc ggtaaaacct ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc
    43081 gatgacttta ggcgatcaat ataagatcgc cgggccacgc aaagaactgc accctccggt
    43141 gcaaatggga tggtaaggag tttattgtga aaaagtggtt attagcggca ggtctcggtt
    43201 tagcactggc aacttctgct caggcggctg acaaaattgc aatcgtcaat atgggcagcc
    43261 tgttccagca ggtagcgcag aaaaccggtg tttctaacac gctggaaaat gagttcaaag
    43321 gccgtgccag cgaactgcag cgtatggaaa ccgatctgca ggctaaaatg aaaaagctgc
    43381 agtccatgaa agcgggcagc gatcgcacta agctggaaaa agacgtgatg gctcagcgcc
    43441 agacttttgc tcagaaagcg caggcttttg agcaggatcg cgcacgtcgt tccaacgaag
    43501 aacgcggcaa actggttact cgtatccaga ctgctgtgaa atccgttgcc aacagccagg
    43561 atatcgatct ggttgttgat gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca
    43621 tcactgccga cgtactgaaa caggttaaat aagtaatgcc ttcaattcga ctggctgatt
    43681 tagcgcagca gttggatgca gaactacacg gtgatggcga tatcgtcatc accggcgttg
    43741 cgtccatgca atctgcacaa acaggtcaca ttacgttcat ggttaaccca aaataccgtg
    43801 agcatttagg cttgtgccag gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg
    43861 cgaaaagtgc cgcgctggta gtgaagaatc cctacctgac ttacgcgcgc atggcgcaaa
    43921 ttttagatac cacgccgcag cccgcgcaga acattgcacc cagtgcggtg atcgacgcga
    43981 cggcgaagct ggggaacaat gtatcgattg gcgctaacgc ggtgattgaa tccggcgttg
    44041 aactgggcga taacgtgatt atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg
    44101 gtgcaggttc gcgtctctgg gcgaacgtaa ccatttacca tgagatccag atcggtcaga
    44161 attgcctgat ccagtccgga acagtggtag gcgcagacgg ctttggttat gccaacgatc
    44221 gtggtaactg ggttaagatc ccacagattg gtcgcgtaat tattggcgat cgcgtggaga
    44281 tcggtgcctg cactaccatc gatcgcggcg cactggatga cactgttatt ggcaatggcg
    44341 tgatcattga taaccagtgc cagattgcac ataacgtcgt gattggcgac aatacggcgg
    44401 ttgccggtgg cgtcattatg gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg
    44461 gagccagcgt aatcaacggg catatggaaa tatgcgacaa agtgacggtt acgggcatgg
    44521 gtatggtgat gcgtcccatc actgaaccag gcgtctattc ctcaggcatt ccgctgcaac
    44581 ccaacaaagt ctggcgcaaa accgctgcac tggtgatgaa cattgatgac atgagcaagc
    44641 gtctgaaatc gcttgagcgc aaggttaatc aacaagacta acgttccatc ttttgttcgc
    44701 caaactttac ggcctgtctc attcttacga ttgcggcagg ccgtgttatt attgtcgttt
    44761 cttatatttt gacaggaaga gtatcttgac tactaacact catactctgc agattgaaga
    44821 gattttagaa cttctgccgc accgtttccc gttcttactg gtggatcgcg tgctggattt
    44881 tgaagaaggt cgttttctgc gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca
    44941 gggccatttc cctggaaaac cgattttccc gggtgtgctg attctggaag caatggcaca
    45001 ggcaacaggt attctggcgt ttaaaagcgt aggaaaactg gaaccgggtg agctgtacta
    45061 cttcgctggt attgacgaag cgcgcttcaa gcgcccggtc gtgcctggcg atcaaatgat
    45121 catggaagtc actttcgaaa aaacgcgccg cggcctgacc cgttttaaag gggttgctct
    45181 ggtcgatggt aaagtagttt gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg
    45241 atacgtgatt gataaatccg cctttgtgca tccaaccgcc attgtggaag agggcgcgtc
    45301 aattggcgcg aacgcacaca ttggtccttt ttgtatcgtt ggaccccatg tcggaattgg
    45361 tgagggtacc gtactgaaat ctcacgttgt cgtgaatggt catactaaaa ttggccgcga
    45421 taatgagatt tatcagttcg cctccatcgg cgaagttaac caggatctga aatatgctgg
    45481 cgaaccgacc cgtgtggaaa tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca
    45541 tcgtggcaca gtccagggcg gtggattgac gaaggtgggc agcgacaact tactgatgat
    45601 caacgcgcac attgcgcacg attgtacggt aggtaaccgc tgtattctcg ccaacaacgc
    45661 aacgctggcg ggtcacgtat cggttgacga cttcgcgatc atcggcggca tgaccgcagt
    45721 ccatcagttc tgcatcattg gtgcgcacgt gatggttggc ggctgctccg gtgtggcgca
    45781 ggacgtccct ccttatgtca ttgcgcaggg taaccacgca acgccgttcg gtgtcaatat
    45841 cgaagggctg aagcgccgcg gattcagccg tgaggcgatt accgctatcc gcaatgcgta
    45901 taagctgatt tatcgtagcg gtaaaacgct cgatgaagtg aaaccggaaa ttgctgaact
    45961 ggcggaaaca tatccggaag tgaaagcctt taccgatttc ttttcacgct caacgcgcgg
    46021 tctgattcgt taatgactga acagcgtcca ttaacgattg ccctggtcgc cggagaaacc
    46081 tccggcgata tcctgggggc cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc
    46141 cgctttgttg gtgttgccgg gccacgaatg caggctgaag gctgcgaagc ctggtacgaa
    46201 atggaagaac tggcggtgat gggcattgtt gaagtgctcg gtcgtctgcg tcgcttactg
    46261 catattcgtg ccgatctgac aaagcgtttt ggcgaactga agccagatgt ttttgttggt
    46321 attgatgcgc ctgacttcaa tattactctt gaaggtaacc tcaaaaagca gggtatcaaa
    46381 accattcatt acgtcagtcc gtcagtctgg gcgtggcgac agaaacgtgt tttcaaaata
    46441 ggcagagcca ccgatctggt gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa
    46501 tacaacgtac cgtgccgctt tatcggtcat accatggctg atgccatgcc attagatcca
    46561 gataaaaatg ccgcccgtga tgtgctgggg atccctcacg atgcccactg cctggcgttg
    46621 ctaccgggga gccgtggtgc agaagttgaa atgcttagtg ccgatttcct gaaaacggcc
    46681 cagcttttgc gccagacata tccggatctc gaaatcgtgg tgccactggt gaatgccaaa
    46741 cgccgcgagc agtttgaacg catcaaagct gaagtcgcgc gagacttttc agttcatttg
    46801 ctggatggga tgggccgtga ggcgatggtc gccagcgatg cggcgctact ggcgtcgggt
    46861 acggcagccc tggagtgtat gctggcgaaa tgcccgatgg tggtgggata tcgcatgaag
    46921 ccttttacct tctggttggc gaagcggctg gtgaaaactg attatgtctc gctgccaaat
    46981 ctgctggcgg gcagagagtt agtcaaagag ttattgcagg aagagtgtga gccgcaaaaa
    47041 ctggctgcgg cgctgttacc gctgttggcg aacgggaaaa tcagccacgc gatgcacgat
    47101 accttccgtg aactgcatca gcagatccgc tgcaatgccg atgagcaggc ggcacaagcc
    47161 gttctggagt tagcacaatg atcgaatttg tttatccgca cacgcagctg gttgcgggtg
    47221 tggatgaagt cggacgcggg ccgttagttg gcgcggtcgt caccgctgcg gtgatccttg
    47281 acccggcgcg cccgattgcc gggctgaatg attccaaaaa gctgagcgaa aaacgccgtc
    47341 tggcgctctg tgaagagatc aaagagaaag cgttgagctg gagtctgggc cgcgcggaac
    47401 cccacgaaat cgacgagctg aacattcttc atgcgaccat gctggcgatg cagcgtgccg
    47461 tcgctgggct gcatattgcg ccggaatatg tgttgattga tggtaaccgc tgcccgaaat
    47521 taccgatgcc tgcgatggct gtggtgaaag gcgatagccg cgtaccggaa atcagtgccg
    47581 cgtctatcct ggcgaaagtg acgcgtgacg ccgaaatggc ggcgctggat attgttttcc
    47641 cgcaatatgg ttttgcccaa cacaaagggt acccaaccgc ttttcatctg gaaaaactgg
    47701 ctgaacacgg cgcgaccgaa caccatcggc gcagctttgg gcctgtcaaa cgcgcactgg
    47761 gacttgcgtc ctgattcttg tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac
    47821 cacgtttcgt acacctgcgg gtgcacagcg actactcgat gatcgatggc ctggccaaaa
    47881 ctgcaccgtt ggtaaaaaag gcggcggcgt tgggtatgcc agcactggcg atcaccgatt
    47941 tcaccaacct ttgtggtctg gtgaagttct acggagcggg acatggcgca gggattaagc
    48001 ctatcgtcgg ggcagatttt aacgtccagt gcgacctgct gggtgatgag ttaacccacc
    48061 tgacggtact ggcggcgaac aataccggct atcagaatct gacgttgctg atctcaaaag
    48121 cgtatcagcg cgggtacggt gccgctgggc cgatcatcga tcgcgactgg cttatcgaat
    48181 taaatgaagg gttgatcctt ctttccggcg ggcgcatggg cgacgtcgga cgcagtcttt
    48241 tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg
    48301 atcgctattt tctcgagctg atccgcaccg gcaggccgga tgaagaaagc tatctgcacg
    48361 cggcggtgga actggcggaa gcgcgcggtt tgcccgtcgt ggcgactaac gatgtgcgct
    48421 ttatcgacag cagcgacttt gacgcacacg aaatccgcgt cgcgatccac gacggcttta
    48481 ccctcgacga tcctaaacgc ccgcgtaact attcgccgca gcaatatatg cgtagcgaag
    48541 aggagatgtg tgagctgttt gccgacatcc ccgaagccct tgccaacacc gttgagatcg
    48601 ccaaacgctg taacgtaact gtgcgtcttg gtgaatactt cctgccgcag ttcccgaccg
    48661 gggacatgag caccgaagat tatctggtca agcgtgcaaa agagggcctg gaagagcgtc
    48721 tggccttttt attccctgac gaggaagaac gtgttaagcg ccgcccggaa tatgacgaac
    48781 gtctggagac tgaacttcag gttatcaacc agatgggctt cccgggctac ttcctcatcg
    48841 ttatggaatt tatccagtgg tcgaaagata acggcgtacc ggtagggcca ggccgtggct
    48901 ccggtgcggg ttcactggtg gcctacgcgc tgaaaatcac cgacctcgat ccgctggaat
    48961 ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt ctccatgcct gacttcgacg
    49021 ttgacttctg tatggagaaa cgcgatcagg ttatcgagca cgtagcggac atgtacggtc
    49081 gtgatgcggt atcgcagatc atcaccttcg gtacaatggc ggcgaaagcg gtgatccgcg
    49141 acgtaggccg cgtgctgggg catccgtacg gctttgtcga tcgtatctcg aaactgatcc
    49201 cgcccgatcc ggggatgacg ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa
    49261 tctacgaagc ggatgaagaa gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg
    49321 tcacccgtaa cgccggtaag cacgccggtg gggtggttat cgcgccgacc aaaattaccg
    49381 attttgcgcc gctttactgc gatgaagagg gcaaacatcc ggtcacccag tttgataaaa
    49441 gcgacgttga atacgccgga ctggtgaagt tcgacttcct tggtttgcgt acgctcacca
    49501 tcatcaactg ggcgctggag atgatcaaca agcggcgggc gaagaatggc gagccgccgc
    49561 tggatatcgc cgcgatcccg ctggatgaca agaaaagctt cgacatgctg caacgctcgg
    49621 aaaccacggc ggtattccag cttgaatcgc gcggcatgaa ggatctgatc aagcgtctgc
    49681 aacctgactg cttcgaagat atgatcgcac tggtggcgct gttccgccca ggtccgttgc
    49741 aatcagggat ggtggataac tttatcgacc gtaaacatgg tcgcgaagaa atctcctatc
    49801 cggacgtaca gtggcagcat gaaagcctga aaccggtact ggagccaacc tacggcatca
    49861 tcctgtatca ggaacaggtc atgcagattg cgcaggtgct ttctggttat accctcggtg
    49921 gcgcggatat gctgcgtcgt gcgatgggta agaaaaagcc ggaagagatg gctaagcagc
    49981 gttctgtatt tgctgaaggt gcagaaaaga acggagtcaa cgccgaactg gcgatgaaaa
    50041 tcttcgacct ggtggagaaa ttcgcgggtt acggatttaa caaatcgcac tctgcggcct
    50101 atgctttggt gtcatatcaa acgttatggc tgaaagcgca ctatccggcg gagtttatgg
    50161 cggcggtaat gaccgccgat atggacaaca ccgagaaggt ggtgggcctg gtggatgagt
    50221 gctggcggat ggggctgaaa atcctgccac cagatataaa ctccggtctt taccatttcc
    50281 acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc catcaaaggg gtaggtgaag
    50341 gtccgattga ggccatcatc gaagcccgta ataaaggcgg ctacttccgc gaactgtttg
    50401 atctctgcgc ccgtaccgac accaaaaagt taaaccggcg ggtgctggaa aaactgatca
    50461 tgtccggggc gtttgaccgt cttgggccac accgcgcggc gctgatgaac tcgctgggtg
    50521 atgcgttaaa agcggcagat caacacgcga aagcggaagc tatcggtcag gccgatatgt
    50581 tcggcgtgct ggccgaagag ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt
    50641 ggccggagca ggtggtgtta gatggggaac gtgaaacgtt aggtctgtac cttacgggac
    50701 accctatcaa ccagtattta aaagagattg agcgttatgt cggaggcgta aggctgaaag
    50761 acatgcaccc gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc
    50821 gggttatggt caccaagcgc ggcaatcgta tcggtatctg cacgctggat gaccgttccg
    50881 ggcggctgga agtgatgttg tttactgacg ccctggataa ataccagcaa ttgctggaaa
    50941 aagaccgcat acttatcgtc agcggacagg tcagctttga tgacttcagc ggtgggctta
    51001 aaatgaccgc tcgcgaagtg atggatattg acgaagcccg ggaaaaatat gctagcgggc
    51061 ttgctatctc gctgacggac aggcaaattg atgaccagct tttaaaccga ctccgtcagt
    51121 ctctggaacc ccaccgctct gggacaattc cagtacatct ctactatcag agggcggatg
    51181 cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa
    51241 acgatctccg tggcctcatt ggttcggagc aggtggaact ggagtttgac taatacagga
    51301 atactatgag tctgaatttc cttgattttg aacagccgat tgcagagctg gaagcgaaaa
    51361 tcgattctct gactgcggtt agccgtcagg atgagaaact ggatattaac atcgatgaag
    51421 aagtgcatcg tctgcgtgaa aaaagcgtag aactgacacg taaaatcttc gccgatctcg
    51481 gtgcatggca gattgcgcaa ctggcacgcc atccacagcg tccttatacc ctggattacg
    51541 ttcgcctggc atttgatgaa tttgacgaac tggctggcga ccgcgcgtat gcagacgata
    51601 aagctatcgt cggtggtatc gcccgtcttg acggtcgtcc ggtgatgatc attggtcatc
    51661 aaaaaggtcg tgaaaccaaa gaaaaaattc gccgtaactt tggtatgcca gcgccagaag
    51721 gttaccgcaa agcactgcgt ctgatgcaaa tggctgaacg ctttaagatg cctatcatca
    51781 cctttatcga caccccgggg gcttatcctg gcgtgggcgc agaagagcgt ggtcagtctg
    51841 aagccattgc acgcaacctg cgtgaaatgt ctcgcctcgg cgtaccggta gtttgtacgg
    51901 ttatcggtga aggtggttct ggcggtgcgc tggcgattgg cgtgggcgat aaagtgaata
    51961 tgctgcaata cagcacctat tccgttatct cgccggaagg ttgtgcgtcc attctgtgga
    52021 agagcgccga caaagcgccg ctggcggctg aagcgatggg tatcatcgct ccgcgtctga
    52081 aagagctgaa actgatcgac tccatcatcc cggaaccgct gggcggcgct caccgtaacc
    52141 cggaagcgat ggcggcatcg ttgaaagcgc aactgctggc ggatctggcc gatctcgatg
    52201 tgttaagcac tgaagattta aaaaatcgtc gttatcagcg cctgatgagc tacggttacg
    52261 cgtaattcgc aaaagttctg aaaaagggtc acttcggtgg ccctttttta tcgccacggt
    52321 ttgagcaggt tatgattaag gaaggatttt ccaggaggaa cacatgaaca tcattgccat
    52381 tatgggaccg catggcgtct tttataaaga tgagcccatc aaagaactgg agtcggcgct
    52441 ggtggcgcaa ggctttcaga ttatctggcc acaaaacagt gttgatttgc tgaaatttat
    52501 cgaacataac cctcgaattt gcggcgtgat ttttgactgg gatgagtaca gtctcgattt
    52561 atgcagcgat atcaatcaac ttaatgaata tctcccgctt tatgccttca tcaacaccca
    52621 ctcgacgatg gatgtcagcg tgcaggatat gcggatggcg ctctggtttt ttgaatatgc
    52681 gctggggcag gtggaagata tcgccattcg tatgcgtcag tacaccaacg aatatcttga
    52741 taacattacg ccgccgttca cgaaagcctt gtttacctac gttaaagagc ggaagtacac
    52801 cttttgtacg ccggggcata tgggcggcac cgcatatcaa aaaagcccgg ttggctgcct
    52861 gttttatgat tttttcggcg ggaataccct caaggctgat gtctctattt cggtcaccga
    52921 gcttggttcg ttgctcgacc acaccgggcc acacctggaa gcggaagagt acatcgcgcg
    52981 gacttttggc gcggaacaga gttatatcgt taccaacgga acatcgacgt cgaacaaaat
    53041 tgtgggtatg tacgccgcgc catccggcag tacgctgttg atcgaccgca attgtcataa
    53101 atcgctggcg catctgttga tgatgaacga tgtagtgcca gtctggctga aaccgacgcg
    53161 taatgcgttg gggattcttg gtgggatccc gcaccgtgaa tttactcgcg acagcatcga
    53221 agagaaagtc gctgccacca cgcaggcaca atggccggtt catgcggtga ttaccaactc
    53281 cacctatgat ggcttgctct acaacaccga ctggatcaaa cagacgctgg atgtcccgtc
    53341 gatccacttc gattccgcct gggtgccgta cacccatttt catccgatct accaggggaa
    53401 aagtgatctt cgaaacgcaa tcgacccaca aaatgctggc ggcgttatcg caggcttcgc
    53461 tgatccacat taaaggcgag tatgacgaag aggcgtttaa cgaagccttt atgatgcata
    53521 ccaccacctc gcccagttat cccattgttg cttcggttga gacggcggcg gcgacgctgc
    53581 gtggtaatcc gggcaaacgg ctgattaacc gttcggtgga gcgggcgctg cattttcgca
    53641 aagaggtcca gcggttgcgg gaagagtctg acggttggtt tttcgatatc tggcaaccgc
    53701 cgcaggtgga tgaagccgaa tgctggcccg ttgcgccagg cgaacagtgg cacggcttta
    53761 acgatgcgga tgccgatcat atgtttctcg atccggttaa agtcactatt ttgataccgg
    53821 ggatggacga acagggcaat atgagcgagg aggggatcct ggcggcgctg gtggcaaaat
    53881 tcctcgacga acgtgggatc gtagtagaga aaaccggccc ttataacctg ctgtttctct
    53941 ttagtattgg tatcgataaa accaaagcaa tgggattatt gcgtgggttg acggaattta
    54001 aacgctctta cgatctcaac ctgcggatca aaaatatgct acccgatctc tatgctgaag
    54061 atcccgattt ctaccgcaat atgcgtattc aggatctggc gcaagggatc cataagctga
    54121 ttcgtaaaca cgatcttccc ggtttgatgt tgtgggcatt cgatactttg ccggaaatta
    54181 tcatgacgcc acatcaggca tggcagagac agattaaagg tgaagtagaa accattgcgc
    54241 tggaacaact ggtcggtaga gtatcggcaa atatgatcct gccttatcca ccgggcgtac
    54301 cgctgttgat gccaggagaa atgctgacca aagagagccg cacggtactc gattttctac
    54361 tgatgctttg ttccgtaggg caacattacc ccggttttga aacggatatt cacggcgcga
    54421 aacaggacga agacggcgtt taccgcgtac gagtcctaaa aatggcggga taacttgcca
    54481 gagcggctta cgggcgagta acgtgctgtt aacaaataaa ggagacgtta tgctgggttt
    54541 aaaacaggtt caccatattg cgattattgc gacggattat gcggtgagca aagctttcta
    54601 ctgcgatatt cttggtttca cgctgcaaag cgaagtctat cgcgaagcgc gcgactcgtg
    54661 gaaaggggat ttggcgctta atgggcaata tgtgattgag cttttctctt ttccgttccc
    54721 gccggagcgc cccagccgac cggaagcttg cggcctgcgt catctggctt ttagcgttga
    54781 tgacatcgat gcggcagtgg cgcaccttga aagccataac gtgaagtgtg aagccatccg
    54841 tgtcgatcca tacacgcaaa aacgcttcac cttctttaac gatccggacg ggctgccgtt
    54901 ggaactgtat gagcagtaag gcttgtcatc gccgcatttg cccggtaacg tgccgggcat
    54961 tgctactgta aaatcgcacc atcatgacac tcacgctcaa tagacaactt ctcacctcac
    55021 gccagatttt ggtcgccttt agcggcgggc ttgactccac cgttctgctg catcagttgg
    55081 tgcagtggcg gacggaaaat ccgggcgtca ctctgcgcgc tattcatgtg catcacggtt
    55141 taagtgccaa tgccgatgcc tgggttacgc attgcgaaaa cgtctgccaa cagtggcagg
    55201 tgccgctggt ggtcgaacgc gtacaacttg cgcaagaggg gctggggatt gaggcccagg
    55261 cgcggcaggc gcgttatcag gcatttgccc gtaccctatt gcccggcgaa gtgctggtca
    55321 ccgcgcaaca tctcgacgat caatgtgaaa cctttctgct ggcgctaaaa cgcggtagcg
    55381 gccctgccgg gctttcggct atggcggaag tttcggagtt tgccggaaca cggcttatcc
    55441 gcccgttgct tgcccgcacg cggggggaac tggcgcagtg ggcattggcg catggtttac
    55501 gctggattga agacgaaagt aatcaggacg actcatacga tcgtaacttt ctgcgcctgc
    55561 gcgtagtgcc gttattgcag cagcgttggc cgcattttgc ggaagcaacg gcccgcagcg
    55621 ccgcactttg tgctgaacaa gagagtctgt tggatgaact gctggcggat gatttagcac
    55681 actgccaaac gtcgcagggg acgctgcaga ttgcgccaat gctggcgatg agtgatgccc
    55741 gccgcgcggc gattatccgt cgctggctgg cagggcagaa tgcaccaatg ccttcccgcg
    55801 acgcgctggt gcggatctgg caggaagtgg cgctggcgcg ggaagatgcc tccccctgtt
    55861 tacgcttggg cgcgtttgaa atccgccgct atcagtcgca actgtggtgg attaaatccg
    55921 tcaccgggca aagtgaaacc attgtgccgt ggcagacgtg gctacaaccg ctggaattac
    55981 cggcggggct gggaagcgta cagcttactg cgggaggcga tattcgccct ccgcgtgcag
    56041 acgaagcggt cagcgtgcgt ttcaaatcgg caggattgct gcatattgtc gggcgcaacg
    56101 gcggacgtaa gctgaagaaa atctggcaag agttaggcgt gccgccgtgg ctacgtgaca
    56161 ccacgccact gctgttttat ggcgaaacgc tgattgcggc ggcaggggta tttgtgacgc
    56221 aagaaggtgt ggctgaaggt gagaatggcg taagttttgt ctggcagaaa acgcttagtt
    56281 aagtgaaagc cggataagac gcatcaaacg tcgcatccgg cgaaagtcaa tcaggactcg
    56341 ctcaccacca ccgtaccgat ttccgggtgg ctaaagctgg taattttatc cagacgtagc
    56401 tcacgggttt cgccagcggc ctcgacgacc agatactcca catttttgcg ggagactaaa
    56461 tcgctggctt ttgcctgcaa tttttcgcca tctttcagct caagtgtcag cattaaatga
    56521 tgctggcagg cgagctcaag attatcgtaa tcatcacaat tgattggttg atacgtatca
    56581 ttcattaaca taatcgctca ccagtaagtt tgccgcagcg tatgctgctt tttccctgac
    56641 agcctcagaa agggcgtcgt cggcagccat ttcattcagc actttcaaaa cgcagcccag
    56701 cgcgtccgga acgtatccta agtctccgct ggcgatttcc gcgtaccgct tgcgtattaa
    56761 ctcacaatat ttttccacat gccctcctgt cagcacactg acttaaccgt ggatgcaagt
    56821 ctaagcctac gaagataaac tctgtttcgc aaggtgacta taccacactc atttctgcaa
    56881 tatcagcgcc gcaactgcac gtattccgtt acaatggcct cctgattcga aaggagtttt
    56941 cttatggcgc ttaaagcgac aatttataaa gcgacggtta atgtggccga tctcgaccgc
    57001 aaccagtttc tcgatgcctc tctgacgctg gcgcgccacc cttcagaaac ccaggagcgt
    57061 atgatgctgc gcctgctggc gtggctgaaa tatgctgatg aacgtctgca atttacccgt
    57121 ggtttgtgtg ccgatgatga gccggaagcg tggctgcgta acgatcacct gggcattgat
    57181 ttgtggattg agctggggct gccggatgag cggcggatta agaaagcctg cacccaggcc
    57241 gcagaagtgg cgctgtttac ctataatagt cgggcggcgc aaatctggtg gcagcaaaat
    57301 cagagcaaat gtgtgcagtt tgccaatctt tccgtctggt atctggacga tgaacaactg
    57361 gcgaaagtaa gcgcctttgc cgatcgtacc atgacgctgc aggcaacgat tcaggatggc
    57421 gtgatctggt tatcggacga taagaataat ctggaagtta acttaaccgc ctggcaacaa
    57481 ccttcatgat tgtgatttcc cgacatgttg ctattcccga tggtgagctt gagatcaccg
    57541 ccattcgtgc gcagggcgcg ggcgggcagc atgttaataa gacctcaacg gccattcatc
    57601 tgcgttttga cattcgggcg tccagcctgc cagagtatta caaagagcgt ctgctcgccg
    57661 ccagccatca tttgatcagc agtgatggcg tgattgtcat taaggcacag gaataccgca
    57721 gtcaggaact gaaccgcgaa gccgcgctag cccggctggt ggcagtgatt aaagatttaa
    57781 caacagaaca aaaagcccga cgacccacgc ggcccacccg tgcatcgaaa gagcgcaggc
    57841 tggcatcgaa agcacaaaaa tcaagcgcga aggcgatgcg cggcaaagta cgcagcggtc
    57901 gggaataaaa agaaggaata gatggtgaaa aaagcgatag tgacagcgat ggctgtaatc
    57961 agcctcttta ctctgatggg atgtaataac cgggccgaag tcgatacgct ttctccagca
    58021 caggctgccg aactgaaacc gatgccgcaa agttggcgcg gcgtgctgcc gtgtgctgat
    58081 tgtgaaggaa tcgaaacctc tctgttcctc gaaaaagacg gaacatgggt gatgaatgag
    58141 cgttatctcg gtgctcgtga agaaccttcc tccttcgctt cctacggtac atgggcgcga
    58201 accgctgaca agctggtatt aaccgacagc aaaggtgaaa agtcatatta tcgggcgaaa
    58261 ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccga ttgaatcgca gttcaactat
    58321 acgctggaac cggcacaatc cagtttacct atgacgccga tgaccctgcg aggcatgtat
    58381 ttttatatgg ctgatgcggc gaccttcact gattgcgcga ccggaaaacg tttcatggta
    58441 gcgaataacg cagagctgga gcgtggctac ctggctgcgc gcggtcacag tgaaaaaccg
    58501 gtgttactgt cagtagaagg tcactttacg cttgaggcta atccggatac tggtgcgcca
    58561 accaaagtat tagcgcccga tacggcgggt aaattttacc cgaataagga ttgcagtagt
    58621 ttggggcagt aaacctgctt aaggcagaaa caaacgcaaa acaggccaga aggatatatt
    58681 tcaacatttt gaatttgcac gttttttgta ggccggataa ggcgtttacg ccgcatccgg
    58741 caatggtgct caacgcctga tgcgacgctg gcgcgtctta tcatgcctac aacccccctc
    58801 ataccttaag gcggataagg catttacttt atcaccgggt caaaccaaca aagcgacccg
    58861 ccttgatata aatccctggc ttcaggtgcc caacatactg taactgcgtt tctggcttaa
    58921 acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc cagcccgatt
    58981 gcgccagtgt aagtggatgt ccggctttgg caaatgcatc agtgacaaat tccgaacaaa
    59041 accacgactt tttgtctcct tcgcccacac tgcttaactg cgctttcgcc aggccgctga
    59101 cgcactgctg gcgaaaatcc tcggagaact gattcagtga gcacatctgg cgagtcacca
    59161 taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg attttattgg
    59221 caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa
    59281 gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg
    59341 cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga
    59401 agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt
    59461 tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt tgatgctgga
    59521 atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg gctggctgat
    59581 atcaacggta caagccgaaa gtaaaagaaa gctggggaga agcaggcggc agtacgcctt
    59641 tggtttatcc attttataca atccatgtaa aaaagggccc tgaaattcag gaccctttct
    59701 ggcatcagcc tttaatctgt ttcaccagat attcgacgat gtcaccagtc ttaattaact
    59761 gtttctcgcc gttgcgacga tatttatatt cgatatcgtc gttgtcgagg ttacggtcgc
    59821 ccagcacaat agtgtgcgga ataccgatca gttccatatc agcaaacatc acgcccggac
    59881 gctctttgcg gtcatccagc agcacttcga taccttgtgc acgcagttcg ctgtacagtt
    59941 tctcagcaag ctcttgtacg cggaaggatt tgtgcatgtt catcggcaga atcgccacct
    60001 ggaacggcgc gatagcgtca ggccatacga tgccgcgttc gtcgtagttc tgctcaatcg
    60061 ccgcagctac cacacgcgtt accccgatac cgtagcaacc catcgtcagg atttggttac
    60121 ggccatcttc accctgtacg gaggctttca gtgcttcgga gtacttggta cccagctgga
    60181 agatgtgacc aacttcgata ccacgtttga tcagcagcgt accctggcca tccgggcttg
    60241 gatcgccagc caccacgtta cggatatctg caacttccgg ggtagcgaca tcgcgatccc
    60301 agttgatacc gaagtagtgt ttaccatcga tgttagcacc agcagcgaaa tcactcatcg
    60361 ccgcaacggt acggtcaatc accaccggaa tcggcatgtt taccggaccc agtgaacccg
    60421 gaccggcttt aaccacagca cgaatttctt cttcggtcgc gaaagtcagc gggcttgcaa
    60481 cctgcggcag tttttctgct ttaacttcgt tcagctcgtg atcaccgcgc accagcagcg
    60541 caaccagcgg gaagctgctg ccttcaaccg ctttaaccag cagagtctta accgttttct
    60601 caatcggcag attgaactgt tcaaccagtt ccgcgatggt tttcgcgttc ggcgtatcaa
    60661 ccagcgtcat ttcctgggta gcagcagcgc gcggttcttt cggcgcgata gcttctgcca
    60721 gttcaatgtt cgctgcatag tcagaggtgt cggagaagac cacatcgtct tcaccgctct
    60781 gcgccagcac ctggaattcg tgagaggcgc tgccgccgat agaaccggtg tcggcttgca
    60841 cggcgcggaa atccagcccc atgcggctga agattttgct gtaggccgca tacattgcat
    60901 cgtaggtttc ctgcagggat tcctgagaag tatggaaaga gtaagcatct ttcatcagga
    60961 attcgcggga acgcatgacg ccgaaacgcg gacgcacttc gtcgcggaac ttggtctgga
    61021 tctgatagaa gttcagcggc agctgtttgt aagagctaag ctcgttacga atcaggtcag
    61081 tgataacttc ttcatgagtt gggccgagta cgaacggacg ctcgccacgg tcaacaaaac
    61141 gcagcagttc cggaccgtac tgttcccaac gaccactctc ttgccacaaa tcggctggct
    61201 gaaccaccgg catcgacacc tcgattgcac cggcgttgtt catctcttca cgcacgatgt
    61261 tttcgacttt tttcagaacg cgcacgccgg tcggcagcca ggtatataac ccggaggcca
    61321 gcttgcggat catcccggcg cgcagcatca gctgatggct gatcacctcg gcgtcggcag
    61381 gtgtctcctt gagagtggag agcaggtatt ggctagtacg catgttgtta cggttccagt
    61441 tggaaggtag aacaggctca aggcgagcct gggacaaaaa aagtgattta gtttaccagt
    61501 gcaaaagaaa tgtcaaaaga gaagggcgtg aatttaacgc ggttccagcg caaagacttc
    61561 aaaacctgcg tcggtgacgc gccagcgaac gttaaaatca tgtagccaga cggcataggt
    61621 tttgcccgtt tcctcacctt tatgataggc cgggcgcggg tcctgcgcca gtacttcgcg
    61681 gataaacagc gttaactgcg gataacgctt ctccagcgtc aaaagctgct tttcgacctc
    61741 tgcggtaaaa ctcaccgcca tctctgcagc tggcgcactt tgcgcatagc tggcactggc
    61801 atcgggaagc gattcggcaa aggggagata cggtttgata tccactaccg gcgtaccatc
    61861 gaccagatcc agactgccga gcttcagaat cacgctgtct ttatggcaaa caacctcttt
    61921 cagctctacc agcgacatac caattgggtt agggcggaaa gtagagcgtg ttgcgaaaac
    61981 ccccattctg gcgttaccgc cgaggcgcgg tggacgcaca gtcggacgcc agccgccttc
    62041 catcgtttga tgaaagacga aaaggatcca taaatggctg aacgcttcca ggccgcgaac
    62101 ggcgtcggcc tggttgtagg gagcaatgag atgcagttcg ccgttggcgc tttttaccag
    62161 acctggctgg cgcggaacgg cgaatttttc tttatagggc gagcgaataa cgcctatttg
    62221 ctcgaactgg aaactgctca tttcgccgta atgttaagcg cagagccgat acatacagcc
    62281 tgacgatagc agcctggcgt accgctggtg acttcgcagc tatgcagtaa taccgcattg
    62341 gctttcattt tagaggcgtt gatttgcatc cgcttacgcg cggttggaat gctcggcgga
    62401 gagtcctgat tagaggcctg gcaagagtcg ccactgactt caccgagatc gcggaacggt
    62461 ttgccgacta attcttctgc attggtataa attcggaccg gcgtggcgcg cggcgctttc
    62521 ggttttgcag gctccgcttt cggctggggt gcagtgcttt gaacgggttc gacaggggat
    62581 ctgcttaaca tggaacagcc gcttagcatg agtgctacta aacagatcgg taaagcacgc
    62641 atagtatttc ctcaatgtat gatcaaaacg tcaatattga atcaggagct tgtaaaaatg
    62701 acaagacggg caagtgccca tcctgaatga tattacaaat tgtggaaaca gcctaaaaat
    62761 taccagcctt taacagcgcc gccgttaaac actttgtttg ctgcttcgta aacttcatca
    62821 gactgataag cctggacgaa tttcttcacg ttctcggcgt ctttgttatc ttcacgcgtt
    62881 acgatcaggt ttacgtacgg ggagtcttta tcttcgacaa agataccgtc tttcgctgga
    62941 gtcaggccaa tctggctggc ataggtggta ttgataactg ccagagcgat ttgtgcgtcg
    63001 tccagagatc gaggcagctg cggtgcttcc agctcaacaa ttttcagatt ttttgggttc
    63061 tcaacaacat caagtacggt cggcagcagg ccaacgccat ctttcagttt gatcaagccc
    63121 actttttgca gcagcagcag tgaacgacca aggttagttg gatcgtttgg cacggcaacc
    63181 tgcgaaccat cctgcagttc atccagtgat ttgattttct tggagtaacc agcaatcgga
    63241 taaacaaaag tgttgcctac tgcgaccagt ttgtagccac gatctttcag ttgctgatca
    63301 aggtacggtt tatgctggaa ggcgttggcg tcgatatcgc ctttgctcaa tgcttcgttt
    63361 ggcagaacat agtcgttgaa ggttaccagc tcaacgtcca ggccatattt gtctttcgca
    63421 actttctgcg cgacttctgc aacctgctgt tcggcaccaa caatcacgcc gactttaatg
    63481 tggtttggat ctttttcatc ctgaccgcag cctaccagtg ccagtgatcc aatcagggct
    63541 cccactgccg caaaggtttt gaatttgaac gccatacctt attccttctt caattattta
    63601 tgttgtgttg aacgttactt gcgagtgaca gcccggacga tgcggtcgcc tgcgaactga
    63661 attaaataaa ccagaatgac cagcaatacc agtaccgtat tcatcaccgt cgcgttatag
    63721 ccgatgtagc catactgata gccaatctga cctaaaccac cggcaccgac agcaccaccc
    63781 atcgcggaat aaccgactag ggtaatcagg gtgatagttg ccgcattcac cagacccggc
    63841 aatgcttccg gtaacagcac tttacggacg atctgcatcg gcgtggcacc cattgcacgg
    63901 gaagcttcaa ttaacccggt tgggatctcc agcagagcgt tctcgaccat acgggcaata
    63961 aacggtgctg caccaacggt taacggaaca atcgctgcct gcaaaccaat cgatgtacca
    64021 acaataacgc gggtaaacgg aatcatccat accagcaaga taatgaacgg gatagaacgg
    64081 aaaatgttca caatcgcaga aacggtacga tacagcttcg cgttagcaat aatttgcccc
    64141 ggacgcgtga cataaagcag aacgccaacc ggcagaccaa tcacaaagcc aaaaaaaccg
    64201 gatacgaagg tcattgccag cgtttcccat acgccacgaa ccagcagcca catcatcggc
    64261 tcagacataa cccagtacct ctacttttac atggtgttcc tgcagccagg caatggcggc
    64321 ttgcgtatct tgttgtgtgc cgtgcatttc agtcagcatg atgccgaact taacgccacc
    64381 ggcgtagtcc atctgcgcgc taataatgtt gttgttgacg ttgaaacgac gcgcggtttc
    64441 agaaagcagt ggggcatcga ccgattgacc ggtaaactcc agacgcagca tcggcacgca
    64501 gtcagtaaat ggctccgctt gcagacgttc ctggtaatct tccgggatat ccagatgcag
    64561 ggtcgactga ataaacttct gcgccagcgg cgttttcgga tgcgagaaca cttcacttac
    64621 cgtgtcctgc tcgatcagtt cgccattgct gatgaccgcc acgcaatcac aaatacgctt
    64681 cacaacgtcc atttcgtggg tgatcaacag aatcgtcaaa cccagacggc ggttaatgtc
    64741 tttcagcagt tcgagaatag aacgtgtcgt tgccgggtcc agcgcgctgg tggcttcatc
    64801 acacagcaat actttgggat tgctggctaa tgcacgggca atcgccacac gttgtttctg
    64861 cccaccggaa agattcgacg ggtagctatc atgcttatcg ccaagaccaa ccaatgacag
    64921 caattccgtc acgcgacgtt tgatctcgtc tttcggtgtg ttgtccagct ccagcggcag
    64981 agccacgttg ccaaaaacag tacgcgaaga gagcaggtta aaatgctgga aaatcatacc
    65041 aatctggcgg cgagctttgg tcaactcgga ttctgacagc gtggtcagtt cctggccatc
    65101 gaccagcacg ctaccctcgg ttgggcgctc cagcaggttt acacaacgta taagcgtact
    65161 cttacccgcg cctgaggcac cgataacgcc ataaatttgt ccagctggca catgcaggct
    65221 gacgttgttc aacgcctgga tggtgcgggt gccctggtgg aacactttgg tgatattcga
    65281 aagttttatc attgattatt tattatcgtc attaagttag tcgtggcatc tcgaatgcct
    65341 gaaacgggca acgccgtcaa tgaaatggat gttaaggcat ccagacgtct aaatcaatca
    65401 ggtttatgcg aagagcactt tcttgcaggt cgaaacatgc gatactagcg tcacatgcct
    65461 tattaaggag ctataaaagg tggcaaagag cgtacccgca atttttcttg accgtgatgg
    65521 caccattaat gtcgatcacg gctatgtcca tgagatcgac aactttgaat ttatcgacgg
    65581 tgttattgac gccatgcgcg agctaaaaaa aatgggcttt gtgctggtgg tagtaaccaa
    65641 ccagtctggc attgctcgcg gtaaatttac cgaagcacag tttgaaacgc tgaccgagtg
    65701 gatggactgg tcgctggcgg accgagatgt cgatctggat ggtatctatt attgcccgca
    65761 tcatccgcag ggtagtgttg aagagtttcg ccaggtctgc gactgccgta aaccacatcc
    65821 ggggatgctt ttgtcagcac gcgattattt gcatattgat atggccgctt cttatatggt
    65881 gggcgataaa ttagaagata tgcaggcagc ggctgcggcg aacgtgggaa caaaagtgct
    65941 ggtgcgtacg ggtaaaccta ttacgcctga agcagaaaac gcggcggatt gggtgttaaa
    66001 tagcctggca gacctgccgc aagcgataaa aaagcagcaa aaaccggcat aatgattaaa
    66061 agatgagcga ttgaaataaa aatgcatttt tccacttgtc ttcctgagac gactccctat
    66121 aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    66181 aagagaaaaa tcctgaaatt cagggttgac tctgaaagag gaaagcgtaa tatacgccac
    66241 ctcgcgacag tgcgctaaag tgcgtcgcaa ctgctcttta acaatttatc agacaatctg
    66301 tgtgggcact cgaagatacg gattcttaac gtcgcaagac gaaaaatg
//
LOCUS       DAJTSL010000003        64738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTSL010000003 DAJTSL010000000
VERSION     DAJTSL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.003"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..137
                     /locus_tag="OO433_000155"
     CDS             <1..137
                     /locus_tag="OO433_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6048777.1"
                     /translation="RLYQTVPQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWPQE
                     "
     gene            complement(236..1468)
                     /gene="araJ"
                     /locus_tag="OO433_000156"
     CDS             complement(236..1468)
                     /gene="araJ"
                     /locus_tag="OO433_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6048778.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMFFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAGE
                     TTGVGYSLGVHYLLPDAA"
     gene            complement(1594..4737)
                     /gene="sbcC"
                     /locus_tag="OO433_000157"
     CDS             complement(1594..4737)
                     /gene="sbcC"
                     /locus_tag="OO433_000157"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6048779.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NGSLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVKAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(4734..5936)
                     /gene="sbcD"
                     /locus_tag="OO433_000158"
     CDS             complement(4734..5936)
                     /gene="sbcD"
                     /locus_tag="OO433_000158"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6048780.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALAESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            6126..6815
                     /gene="phoB"
                     /locus_tag="OO433_000159"
     CDS             6126..6815
                     /gene="phoB"
                     /locus_tag="OO433_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6048781.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            6873..8168
                     /gene="phoR"
                     /locus_tag="OO433_000160"
     CDS             6873..8168
                     /gene="phoR"
                     /locus_tag="OO433_000160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6048782.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            8575..9894
                     /gene="brnQ"
                     /locus_tag="OO433_000161"
     CDS             8575..9894
                     /gene="brnQ"
                     /locus_tag="OO433_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6048783.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            9970..11343
                     /gene="proY"
                     /locus_tag="OO433_000162"
     CDS             9970..11343
                     /gene="proY"
                     /locus_tag="OO433_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6048784.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            11499..13221
                     /gene="malZ"
                     /locus_tag="OO433_000163"
                     /pseudo
     CDS             11499..13221
                     /gene="malZ"
                     /locus_tag="OO433_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
     gene            complement(13226..13807)
                     /gene="acpH"
                     /locus_tag="OO433_000164"
     CDS             complement(13226..13807)
                     /gene="acpH"
                     /locus_tag="OO433_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR6048785.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRRETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFI
                     CYARKQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYTALGTRFWQFYPRMMAQASHKAL"
     gene            13900..14970
                     /gene="queA"
                     /locus_tag="OO433_000165"
     CDS             13900..14970
                     /gene="queA"
                     /locus_tag="OO433_000165"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6048786.1"
                     /translation="MRVTDFFFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTSVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            15026..16153
                     /gene="tgt"
                     /locus_tag="OO433_000166"
     CDS             15026..16153
                     /gene="tgt"
                     /locus_tag="OO433_000166"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6048787.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            16176..16508
                     /gene="yajC"
                     /locus_tag="OO433_000167"
     CDS             16176..16508
                     /gene="yajC"
                     /locus_tag="OO433_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6048788.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            16536..18383
                     /gene="secD"
                     /locus_tag="OO433_000168"
     CDS             16536..18383
                     /gene="secD"
                     /locus_tag="OO433_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6048789.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            18394..19365
                     /gene="secF"
                     /locus_tag="OO433_000169"
     CDS             18394..19365
                     /gene="secF"
                     /locus_tag="OO433_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6048790.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGEDLTQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            19494..19841
                     /locus_tag="OO433_000170"
     CDS             19494..19841
                     /locus_tag="OO433_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6048791.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(20018..20902)
                     /gene="tsx"
                     /locus_tag="OO433_000171"
     CDS             complement(20018..20902)
                     /gene="tsx"
                     /locus_tag="OO433_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6048792.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLNFGPFKEWYFANNYIYDMGRNKNGRQSTWYMGLGTDIDTDLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(21201..21740)
                     /locus_tag="OO433_000172"
     CDS             complement(21201..21740)
                     /locus_tag="OO433_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6048793.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGGYLLPRAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            21891..22340
                     /gene="nrdR"
                     /locus_tag="OO433_000173"
     CDS             21891..22340
                     /gene="nrdR"
                     /locus_tag="OO433_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6048794.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            22344..23447
                     /gene="ribD"
                     /locus_tag="OO433_000174"
     CDS             22344..23447
                     /gene="ribD"
                     /locus_tag="OO433_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6048795.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWGEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            23536..24006
                     /gene="ribE"
                     /locus_tag="OO433_000175"
     CDS             23536..24006
                     /gene="ribE"
                     /locus_tag="OO433_000175"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6048796.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            24026..24445
                     /gene="nusB"
                     /locus_tag="OO433_000176"
     CDS             24026..24445
                     /gene="nusB"
                     /locus_tag="OO433_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6048797.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            24523..25500
                     /gene="thiL"
                     /locus_tag="OO433_000177"
     CDS             24523..25500
                     /gene="thiL"
                     /locus_tag="OO433_000177"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6048798.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGVRIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            25478..25996
                     /gene="pgpA"
                     /locus_tag="OO433_000178"
     CDS             25478..25996
                     /gene="pgpA"
                     /locus_tag="OO433_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6048799.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(26050..27024)
                     /gene="yajO"
                     /locus_tag="OO433_000179"
     CDS             complement(26050..27024)
                     /gene="yajO"
                     /locus_tag="OO433_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6048800.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(27079..28941)
                     /gene="dxs"
                     /locus_tag="OO433_000180"
     CDS             complement(27079..28941)
                     /gene="dxs"
                     /locus_tag="OO433_000180"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6048801.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(28966..29865)
                     /gene="ispA"
                     /locus_tag="OO433_000181"
     CDS             complement(28966..29865)
                     /gene="ispA"
                     /locus_tag="OO433_000181"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6048802.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSPDTSALEALADYIIQRNK"
     gene            complement(29865..30107)
                     /gene="xseB"
                     /locus_tag="OO433_000182"
     CDS             complement(29865..30107)
                     /gene="xseB"
                     /locus_tag="OO433_000182"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6048803.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            30313..31761
                     /gene="thiI"
                     /locus_tag="OO433_000183"
     CDS             30313..31761
                     /gene="thiI"
                     /locus_tag="OO433_000183"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6048804.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIALQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(31815..32405)
                     /gene="yajL"
                     /locus_tag="OO433_000184"
     CDS             complement(31815..32405)
                     /gene="yajL"
                     /locus_tag="OO433_000184"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6048805.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLQVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(32368..33279)
                     /gene="panE"
                     /locus_tag="OO433_000185"
     CDS             complement(32368..33279)
                     /gene="panE"
                     /locus_tag="OO433_000185"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6048806.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSANLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            33447..33938
                     /gene="yajQ"
                     /locus_tag="OO433_000186"
     CDS             33447..33938
                     /gene="yajQ"
                     /locus_tag="OO433_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6048807.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(34066..35430)
                     /locus_tag="OO433_000187"
     CDS             complement(34066..35430)
                     /locus_tag="OO433_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6048808.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(35579..36469)
                     /gene="cyoE"
                     /locus_tag="OO433_000188"
     CDS             complement(35579..36469)
                     /gene="cyoE"
                     /locus_tag="OO433_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6048809.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(36481..36810)
                     /locus_tag="OO433_000189"
     CDS             complement(36481..36810)
                     /locus_tag="OO433_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6048810.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIITILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(36810..37424)
                     /locus_tag="OO433_000190"
     CDS             complement(36810..37424)
                     /locus_tag="OO433_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6048811.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTCLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(37414..39405)
                     /gene="cyoB"
                     /locus_tag="OO433_000191"
     CDS             complement(37414..39405)
                     /gene="cyoB"
                     /locus_tag="OO433_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR6048812.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWANSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(39427..40374)
                     /gene="cyoA"
                     /locus_tag="OO433_000192"
     CDS             complement(39427..40374)
                     /gene="cyoA"
                     /locus_tag="OO433_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCR6048813.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(40835..42310)
                     /gene="ampG"
                     /locus_tag="OO433_000193"
     CDS             complement(40835..42310)
                     /gene="ampG"
                     /locus_tag="OO433_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6048814.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(42354..42932)
                     /locus_tag="OO433_000194"
     CDS             complement(42354..42932)
                     /locus_tag="OO433_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6048815.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLAASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKITKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            43237..43554
                     /gene="bolA"
                     /locus_tag="OO433_000195"
     CDS             43237..43554
                     /gene="bolA"
                     /locus_tag="OO433_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6048816.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            43898..45196
                     /gene="tig"
                     /locus_tag="OO433_000196"
     CDS             43898..45196
                     /gene="tig"
                     /locus_tag="OO433_000196"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6048817.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDSAVEA
                     EDRVTIDFTGSVDSEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            45441..46064
                     /gene="clpP"
                     /locus_tag="OO433_000197"
     CDS             45441..46064
                     /gene="clpP"
                     /locus_tag="OO433_000197"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6048818.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            46190..47464
                     /gene="clpX"
                     /locus_tag="OO433_000198"
     CDS             46190..47464
                     /gene="clpX"
                     /locus_tag="OO433_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6048819.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            47652..50006
                     /gene="lon"
                     /locus_tag="OO433_000199"
     CDS             47652..50006
                     /gene="lon"
                     /locus_tag="OO433_000199"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6048820.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENCVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            50215..50487
                     /gene="hupB"
                     /locus_tag="OO433_000200"
     CDS             50215..50487
                     /gene="hupB"
                     /locus_tag="OO433_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6048821.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            50679..52550
                     /gene="ppiD"
                     /locus_tag="OO433_000201"
     CDS             50679..52550
                     /gene="ppiD"
                     /locus_tag="OO433_000201"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6048822.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            52701..53072
                     /locus_tag="OO433_000202"
     CDS             52701..53072
                     /locus_tag="OO433_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6048823.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            53166..53564
                     /gene="fadM"
                     /locus_tag="OO433_000203"
     CDS             53166..53564
                     /gene="fadM"
                     /locus_tag="OO433_000203"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6048824.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(53616..54311)
                     /gene="queC"
                     /locus_tag="OO433_000204"
     CDS             complement(53616..54311)
                     /gene="queC"
                     /locus_tag="OO433_000204"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6048825.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMEKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(54376..56076)
                     /locus_tag="OO433_000205"
     CDS             complement(54376..56076)
                     /locus_tag="OO433_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR6048826.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLALG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            56176..56958
                     /gene="cof"
                     /locus_tag="OO433_000206"
                     /pseudo
     CDS             56176..56958
                     /gene="cof"
                     /locus_tag="OO433_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
     gene            57147..57605
                     /gene="decR"
                     /locus_tag="OO433_000207"
     CDS             57147..57605
                     /gene="decR"
                     /locus_tag="OO433_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6048827.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            57635..59407
                     /locus_tag="OO433_000208"
     CDS             57635..59407
                     /locus_tag="OO433_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6048828.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYFLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASENIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            59400..61181
                     /locus_tag="OO433_000209"
     CDS             59400..61181
                     /locus_tag="OO433_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6048829.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            61362..61700
                     /gene="glnK"
                     /locus_tag="OO433_000210"
     CDS             61362..61700
                     /gene="glnK"
                     /locus_tag="OO433_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6048830.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            61730..63016
                     /gene="amtB"
                     /locus_tag="OO433_000211"
     CDS             61730..63016
                     /gene="amtB"
                     /locus_tag="OO433_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6048831.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLMQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQELEGLDVNSHGENAYNA"
     gene            complement(63065..63925)
                     /gene="tesB"
                     /locus_tag="OO433_000212"
     CDS             complement(63065..63925)
                     /gene="tesB"
                     /locus_tag="OO433_000212"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6048832.1"
                     /translation="MSQALKNLLALLNLEKIEAGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            64143..64715
                     /locus_tag="OO433_000213"
     CDS             64143..64715
                     /locus_tag="OO433_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6048833.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
BASE COUNT        15515 a        16205 c        17483 g        15535 t
ORIGIN      
        1 accgtttata tcaaacggtt ccgcagttga ttaaacaatt tgtcttcggc ggcgaatgtg
       61 aaacgccggt gcgtaaggcg aagcacggtg attccagcgg cgtacgcggc gctgcgtggt
      121 tatggccaca agagtaaaaa acgtaggcaa ttggcgcatc atgcctgatg cgacgcttgc
      181 cgcgtcttat caggcctgca aaatgtgcca gaaccgcgta gggcggataa ggcgttcacg
      241 ccgcatccgg caataagtaa tgaacaccga gactataacc taccccagtg gtttcgccag
      301 caagcaccgg agtatccgcc gcttgctggc gcttatagcg accatacagc agcaacgacg
      361 acatcgcagc aaacgaaagc agggcggcag gcagcgccac gtaattatat gccagcccca
      421 gcgtcagcat cataccaccg caatatgcgc cgacggcgct accgaggtta aacgctattt
      481 gcccacctgc ggcacctaat aactctccgc ctttggcgtt ttgtagtaac aatatttgta
      541 gcggtgctga aagggcaaat aatcccgcgc aacaaataaa agcaaaaata agcgacgttg
      601 ttttcatgcc gccgcagaaa aagaacatca gcagtgccag tacaattata aagtcagtca
      661 ctgctgcaat gcgcagtggt gaataacgtc ctgaaatcct gccacttagc atatttccca
      721 gcaccatccc tagcccaact aacatcataa taaaggtcat cgccgtttcc gaaaaaccgg
      781 aaataaacat catgtatggc tttacgtagc tgaaccaggc aaacacacct gcgttgccaa
      841 acatggtggc ggcgaaaatt aaccacgggg ccgggctgcg caaaaagtga aattgttcgc
      901 gcagctttcc tttcgcctcg tcgcgaatat ccggcaccca aaaatagacc gatgccatca
      961 ccgcaatatt aaaaacagcg atcaataaaa aggtgtaacg ccagctaaat tcctgactta
     1021 aatacgttcc cagcggaatg cccagcaaat tggcgactgt catcccggaa accatccccg
     1081 ccacggcggc ggtgactttt ccgggtttga taatttttga taacacgatc gcgccgacgc
     1141 caaaaaatgc gccatgcgga aagccggata ccagccgacc aatggcgagc atcaggtaag
     1201 acgaagagag cgtgaacatg gcgttgccaa tgacgcacaa cgccaccaga aacaacaaga
     1261 tatgtttgag tgagtagcgg ctggaaaaga gtgcgatgat tggcgcaccg accaccactc
     1321 ccagtgcata atacgagatc atatgcccgg cagcaggaat cgaaattcct acgttatgcg
     1381 ccagctccgt gagcacgccc ataatgccaa attcggccat ccccaaacca aacgtgccca
     1441 gagccaaaga taaaatgact tttttcatac caccatcaca accaccagaa cggctaccaa
     1501 tcaccgtagc caataaagtg atagcttgca ggataacgac cagcaaggcc aagagataat
     1561 tcgcccctct gtattcatta tcctgctgaa tagttatttc actgcaaacg tactttccag
     1621 tttgctgtag cccaggccgt tgatcttttt cactttgatc tgcaccggaa tacgctcttt
     1681 catcgcttct acgtggctaa tcacaccgat ggttttgcca ctggcgttca gggcatccag
     1741 cgcatcaagg gcggtatcca gcgtttcgct atccagcgtg ccaaaacctt catcaaggaa
     1801 cagcgagtca atacgtgttt tatggctgac cagatccgaa agcgccagcg ccagcgccag
     1861 actaacgagg aaactttcgc cgccggaaag ggtacgggta tcgcgtaccg catctgcctg
     1921 ccaggtatca acaacctcga cttccagcgc ctcgctggct ttgcgctgta acagatagcg
     1981 cccgtgcagc cgggtaagtt gctgattagc gagatggact aaattatcca gcgtcagccc
     2041 ctgggcaaac ttgcggaatt tatcgccctc tttggaacct attagcgaat tcagatatcc
     2101 ccagtcctca acctgctgcg tcatttgagc aatttgctgc attaaggttt gttgttgctg
     2161 acggttatct gcatcctgct tcagctgctg gcgaatctcg ccttgactcg tggtgttttc
     2221 acgcaacttt tggtgagttt gcgctaactc ttgctgaatc tgctccaccg tcacagtgag
     2281 agccaacccg tcaggtcggt gttgttgatg ctgtgccagc gtttctgctg tctgagtgac
     2341 cagcgtttgc gcctgacggc gctggttttc cagattctgc ttgagctgtt ccagctgcgt
     2401 tagtgtttgt tcatccatta gcgccgcaag gaacgcctgc tgatcgtcaa agacgctggc
     2461 ctgtagcgcg gtgtcaaact gcgcctgggc tttttgcaga ctttgcgccg ccagaacatc
     2521 ctgttgctgt aacgtctgct gctggctgtg taatgcgaga cattgttcat gaacctgccg
     2581 ccagttatcc agcgccacag tttcttcgct gtgcgggaga tcatcacttt gcggcaacgt
     2641 ttccagaatc ggcgtcagct gctgaatacg gttttgcagc gtggttaatt cgttctggcg
     2701 ttgctgccag ctctgcgctt cttgctgacg tgtcgccaac cagctctctt cttcatcttc
     2761 ctgtggcaat gtcagtgcat aacccgccaa tgccgttaag agctgctgct ggcgttgttc
     2821 aatttgctgt tgatactgga taatttgctg attatgcgcg gcaatctgcc cttgtaattc
     2881 atgtcgttgg ctgagtaacc gcagctggcg tttgtgctca tcttgtgcat ccagccacgg
     2941 ttgaatatcg tcctgtggct gcaaggtgat attgaggctg gccgtgacgg cttgccattg
     3001 ttgagtaagt gcttgctcat cttgtcggag gctttgcgct tcgttttcat cgcgctgaag
     3061 ctgcttcgtt aatgcatcca gttgcccgcg cagcgccgca ccttcttcgc cgagcttttt
     3121 aacttcgttt tccagcgcca gtaatcgaga ctgattaaca ccaggctcca gcgcctgata
     3181 cgccttgacc gccgggtggc tggtggaacc acaaagtgga caaggctgac ccgcctgtaa
     3241 ctgtgcacgc tgggcttcca gcgttttgat gcgcgcttcc tgctcgcaaa tggttttcac
     3301 atcggcaagt tgctgcgtct tttctttata acgctggcgc atttcgttaa gtgcgacatt
     3361 acgttgcgtc tgttccagag atccattctg gatagtgacc attaactgcg ccagacgttt
     3421 ttgttgggga acaatctgcc catgcagcgc gaccaggcgc tgacgcagtg ggcgttgctc
     3481 agcatgttgc gccagggcgg tagcaacttc atcggcggtt aacgtcaacg tgatcgccgc
     3541 aagcgcatta agtttttgct cagcatgggt taactgttgc tgccattgcc gcagatgctc
     3601 gcgatcgctg gtttgttggg agaactgcgc acgccaaccc gccagttcgt tgttccactg
     3661 acggaagcgg tcgtgttcct gtaaccaggt attcaggctt tgttgctgct gctgtaattc
     3721 tgctgactgc ttcgccgcgt ggtggcgaat gctcgcgcga agcgccattg tgctctgtaa
     3781 gcgagtattt acttcttcaa tctgctggcg agtatgcgcc agcgccgcgc tgtgttctgc
     3841 gatgcgttcc cagtgcggac gaagatttcg tgccggttgt gccagactga gcgccgccag
     3901 ttgaggttgc gctttttctt cttcggctaa cgcctgttgc aaggcctgct gacggcggct
     3961 ggcttcttgc tgcaattcgt ccagacgcgt taaccagttt agtgattgtt gttcttgctg
     4021 ctgcgcggta agtaactgtt tttcttcgtc agtaagtacc tgcaaactcg ctgtcagcga
     4081 ttgcacttgt tccggcgtga gcaacgcgac gccgctggcc tgcgcttgca gcttctccag
     4141 ctctgtgcgg gccgatttgt gctgctcaaa aaccatcgcc gagatttgcc cgtagatttc
     4201 agtgccggtt aactcctcga gcaattccgc gcgttctttg ggtttggcat tcaggaaggc
     4261 agcaaattgc ccctgcgaaa gcagcatcga acgggtgaag cgcccgtaat ccagcccggt
     4321 taacgtcgct gtcagttcca gcttatcttt cactttgtcg gcgagaattt tgccgtcggc
     4381 gcagcgcgcc agctctacgc gtggcacctg caaattaccg tcgggttggt tacgcgcccg
     4441 attctggctc cagaatgcac ggtacgcttc acctttcact tcaaactcca cctccgccag
     4501 acattcggcg gtatcgcggg tcatgagatc attttgcgat tgtgaaacgt tagagagacg
     4561 cggagtttcg tgatacagcg ccagacaaat ggcgtccagc agggtggttt tccccgcacc
     4621 ggttgggccg gtaatggcaa acagcccgtt gctggcgaac ggctcgcggg tgaaatcaat
     4681 cttccattcg ccttttaatg agttcaggtt tttcaggcgc aggctgagaa ttttcatgct
     4741 tcgtgttctc cggcgagggt atgcaacgtc gtggtgaaaa gatgctgcag acgttgctgc
     4801 tgcgattcat ccagttcttc cagtgccaga cggcgattga acacctcttc gacgctgagt
     4861 tcgctgaggg tttcacgctg ttggctggct aacacacgct cgcgctgttc acgactccga
     4921 cgtaccagca atacttcgac aggcaatgat tcggctaatg cctggatttt gcgctgaata
     4981 tcatgcagat actcatcagt agtgatttcg atatccagcc agacaggtgg ctcctgcgat
     5041 acatcgcgcc actgttccag ctgtgcggta atcgacgcca gatcgccttt cagcaccgcc
     5101 atgggttgag ttaccggtac gttcaggttt tccacgctct ctaatttgcc gtttgaaaat
     5161 gtcaccagat ggacatattt actcttaccg cattcatcaa aactcagtgg aatgggggaa
     5221 ccgcaatagc gaacatgttc catgccgcca ataatctgtg cgcggtgaat atgcccgagc
     5281 gcgatgtagt cggctggtgg aaagttttgt gccggaaacg cgtccagcgt gccaatataa
     5341 atgtcacgca cggcgtcact tttactggca cccacggtcg ttaaatgtcc cgtggcgatg
     5401 atgggcagag gctgatcgcc gcgcagtttg caggcatcgg catagtgttg ttggtaataa
     5461 tcggtaatcg ctgccagtaa atgctgctgt ttttcaatac cgttaagccc cgcctggctg
     5521 gaaataatgt cacgcgggcg taaaaacgga atggggcaca gcactgcgcc tggcgtcccg
     5581 tcgcgacgag gcaagatttg cggcgcatgt ccggcgctgg cgaccacggt tgtattgagg
     5641 aacgccatga tatcgcgcga ttcattcagc gtggcgaccg agtcatggtt tcctgccagt
     5701 accaccagat gacagccagt ttgctgtaaa ttgacaacaa aacggttgta taacgtgcgg
     5761 gcgtaactgg gcggcgagcc ggtatcgaaa acatcaccag caacaataat cgcatccacc
     5821 tgatgggttt gtgctgtctc cagcagccag tcaagaaaag cctgatgttc agcttcgcgg
     5881 cttttactgt agaagttctg gccgagatgc cagtctgagg tgtgaaggat gcgcataacg
     5941 gttccctggc gaaaaagcat ggtcgcgatt atacccaaac agatgtgcca tttgcttttt
     6001 tctgcgccac ggaaatcaat aacctgaaga tatgtgcgac gagcttttca taaatctgtc
     6061 ataaatctga cgcataatga cgtcgcatta atgatcgcaa cctatttatt acaacagggc
     6121 aaatcatggc gagacgtatt ctggtcgtag aagatgaagc tccaattcgc gaaatggtct
     6181 gcttcgtgct cgaacaaaat ggctttcagc cggtcgaagc ggaagattat gacagtgctg
     6241 tgaatcaact gaatgaaccc tggccggatt taattctcct tgactggatg ttacctggcg
     6301 gctccggtat ccagttcatc aaacacctca agcgcgagtc gatgacccgg gatattccag
     6361 tggtgatgtt gaccgccaga ggggaagaag aagatcgcgt gcgcggcctt gaaaccggcg
     6421 cggatgacta tatcaccaag ccgttttcgc cgaaggagct ggtggcgcga atcaaagcgg
     6481 taatgcgccg tatttcgcca atggcggtgg aagaggtgat tgagatgcag ggattaagtc
     6541 tcgacccgac atctcaccga gtgatggcgg gcgaagagcc gctggagatg gggccgacag
     6601 aatttaaact gctgcacttt tttatgacgc atcctgagcg cgtgtacagc cgcgagcagc
     6661 tgttaaacca cgtctgggga actaacgtgt atgtggaaga ccgcacggtc gatgtccaca
     6721 ttcgtcgcct gcgtaaagca ctggagcccg gcgggcatga ccgcatggtg cagaccgtgc
     6781 gcggtacagg atatcgtttt tcaacccgct tttaacgcct tgctcatcgg acgcagagca
     6841 gggcttatga tttcttaact ggagtatctt acgtgctgga acggctgtcg tggaaaaggc
     6901 tggtgctgga gctgctactt tgttgcctcc cggctttcat cctgggtgca ttttttggtt
     6961 acctgccctg gtttttgctg gcatcggtaa caggactgct tatctggcat ttctggaatt
     7021 tattgcgcct ttcatggtgg ttgtgggtgg atcgcagtat gaccccgcca ccggggcgtg
     7081 gtagctggga accgctacta tacggcttac accagatgca gctgcgaaat aaaaaacgcc
     7141 gccgtgaact gggcaatctg attaaacgct ttcgtagcgg cgcggagtcg ctgcccgacg
     7201 cggtggtgct gaccacggaa gagggcggta ttttctggtg taacggtctg gcgcaacaaa
     7261 ttcttggttt gcgctggccg gaagataacg ggcagaacat ccttaaccta ctgcgttacc
     7321 cggagtttac gcaatatctg aaaacgcgtg atttttctcg cccgctcaat ctggtgctca
     7381 acaccgggcg gcatctggaa attcgcgtca tgccttatac ccacaaacag ttgctgatgg
     7441 tggcgcgtga tgtcacgcaa atgcatcaac tggaaggggc gcggcgtaac ttttttgcca
     7501 acgtgagcca tgagttacgt acgccattga ccgtgttaca gggttacctg gagatgatgg
     7561 atgagcagcc gctggaaggc gcggtacgcg aaaaagcgtt gcacaccatg cgcgagcaga
     7621 cccagcggat ggaagggctg gtgaagcagt tgctaacgct gtcgaaaatt gaagccgcgc
     7681 cgacgcaatt gctcaatgaa aaggttgatg tgccgatgat gctgcgcgtt gttgagcgcg
     7741 aggctcagac tctgagtcag aaaaaacaga catttacctt tgagatagat aacggcctca
     7801 aagtgtctgg caacgaagat cagctacgca gtgcgatttc gaacctggtc tataacgccg
     7861 tgaatcatac tccagaaggc acgcatatca ccgtacgctg gcagcgagtg ccgcacggtg
     7921 ccgaatttag cgttgaagat aacggaccgg gcattgcacc ggagcatatt ccgcgcctga
     7981 ccgagcgttt ttatcgcgtt gataaagcgc gttcccggca aaccggcggt agcggattag
     8041 ggttagcgat cgtgaaacat gctgtgaatc atcacgaaag tcgcctgaat attgagagta
     8101 cagtaggaaa aggaacacgt ttcagttttg ttatcccgga acgtttaatt gccaaaaaca
     8161 gcgattaatc cgcctttgtc atcttttatt gccataagcc agtcgatgct ggcttatttt
     8221 ctttgcagtc aaaatacggg cgttagattt tacaacggtt ggtgattttt tgttcgcatg
     8281 attagccatg tctttttcac ggaaatagtg ttttatactg gttggtgatt tcttatcgct
     8341 atatacctct ggtttttaga tccctccttg ctttaaaacg ttataagcgt ttaaattgcg
     8401 cttcaggtgc tgtcatactg actgcattaa cgcggtaaat cgaaaaacta ttcttcgccg
     8461 cgccaggttg ggagtatttc ccgctaaaat tgtttaaata taccgctgta tcatccccag
     8521 ggattggcac aaaaatttaa cgttacaaca ccacatccac aggcagtatg atttatgacc
     8581 catcaattaa gatcgcgcga tatcatcgct ctgggcttta tgacatttgc gttgttcgtc
     8641 ggcgcaggta acattatttt ccctccaatg gtcggcttgc aggcaggcga acacgtctgg
     8701 actgcggcat tcggcttcct cattactgcc gttggcctgc cggtattaac ggtagtggcg
     8761 ctggcaaaag ttggcggcgg tgttgacagc ctcagcacgc caatcggtaa agtcgctggc
     8821 gtgctgctgg caacggtttg ttacttggcg gtggggccgc ttttcgctac gccgcgtaca
     8881 gctaccgttt cctttgaagt ggggattgcg ccgctgacgg gtgattccgc gctgccgctg
     8941 tttatctaca gcctggtcta tttcgctatc gttattctgg tttcgctcta tccgggcaag
     9001 ctgctggata ccgtgggcaa cttccttgcg ccgctgaaaa ttatcgcgct ggtcatcctg
     9061 tctgttgccg ctattgtctg gccggcgggt tctatcagca cggcgactga ggcttatcaa
     9121 aacgctgcgt tttctaacgg cttcgttaac ggctatctga ccatggatac gctgggcgca
     9181 atggtgtttg gtatcgttat tgttaacgcg gcgcgttctc gtggcgttac cgaagcgcgt
     9241 ctgctgaccc gttataccgt ctgggctggc ctgatggcgg gtgttggtct gactctgctg
     9301 tacctggcgc tgttccgtct gggttcagac agcgcgtcgc tggtcgatca gtctgcaaac
     9361 ggcgctgcta ttctgcatgc ttacgttcag cacacatttg gcggcggcgg tagcttcctg
     9421 ctggcggcgt taatcttcat cgcctgcctg gtaacggcag ttggcctgac ctgtgcttgt
     9481 gcagaattct ttgcccagta cgtaccgctc tcttatcgta cgctggtgtt tatcctcggc
     9541 ggcttctcga tggtggtgtc taacctcggc ttaagccagc tgatccagat ctccgtaccg
     9601 gtgctgaccg ctatttatcc gccgtgtatc gcactggttg tattaagttt tacacgctca
     9661 tggtggcata attcgtcccg cgtgattgcc ccgccgatgt ttatcagcct gctttttggt
     9721 attctcgacg ggatcaaagc atctgcattc agcgatatct taccgtcctg ggcgcagcgt
     9781 ttaccgctgg ccgaacaagg cctggcgtgg ttaatgccaa cagtggtgat ggtggttctg
     9841 gccattatct gggatcgcgc ggcaggtcgt caggtgacct ccagcgctca ctaaatcact
     9901 gaacatttgt tttaaccacg gggctgcgat gccccgtggt tttttattgt gttgatgggt
     9961 taggaattga tggaaagtaa gaacaagcta aagcgtgggc taagtacccg ccacatacgc
    10021 tttatggcac tgggttcagc aattggcacc gggctgtttt acggttcggc agacgccatc
    10081 aaaatggccg gtccgagcgt gttgttggcc tatattatcg gtggtatcgc ggcgtatatc
    10141 attatgcgtg cgctggggga aatgtcggta cataacccgg ccgccagctc tttctcgcgt
    10201 tatgcgcagg aaaacctcgg cccgctggca ggttacatta ccggctggac ctactgcttt
    10261 gaaatcctaa ttgtcgccat cgccgatgtg accgcttttg gtatctatat gggtgtctgg
    10321 ttcccgacgg tgccgcactg gatttgggta ctgagcgtgg tgctgatcat ttgcgccgta
    10381 aacctgatga gcgtgaaggt attcggtgag ctggaattct ggttctcgtt ctttaaagtc
    10441 gccaccatca tcatcatgat tgtcgccggt ttcggcatca tcatctgggg gattggcaac
    10501 ggcgggcaac cgaccggtat tcataacctg tggagcaacg gcggcttctt cagtaacggc
    10561 tggcttggca tggtaatgtc gttgcaaatg gtgatgtttg cttacggtgg gatcgaaatt
    10621 atcgggatta ccgccggtga agcgaaagat cctgagaaat cgattccgcg tgcgattaac
    10681 tccgtgccga tgcgtattct ggtgttctac gtcggtacgc tgttcgtcat tatgtctatc
    10741 tacccgtgga atcaggttgg cactgccggt agcccgttcg tgctgacgtt ccagcatatg
    10801 ggcattacct ttgccgccag cattcttaac tttgttgtgc tgaccgcttc gctgtcggca
    10861 attaacagtg acgtatttgg cgtaggccgt atgctccacg gtatggcaga gcagggcagc
    10921 gcaccgaaaa ttttcagcaa aacgtcgcgt cgcggtattc cgtgggttac ggtgctggtg
    10981 atgactaccg cgctgctgtt tgcggtgtat ctgaactaca tcatgccgga aaacgtcttc
    11041 ctggtgatcg cttcgctggc aaccttcgcc acggtatggg tgtggattat gatcctgctg
    11101 tcgcaaatcg ccttccgtcg ccgtttgccg ccagaagaag ttaaggcgct gaaatttaaa
    11161 gtgccgggtg gggtagcaac gaccatcggc gggctgattt tcctgctctt tattatcggg
    11221 ttgattggtt atcacccgga tacgcgtatc tcgctgtatg tcggtttcgc gtggattgtt
    11281 gtgctgttga ttggctggat gtttaagcgt cgccacgatc gtcagctggc tgaaaaccag
    11341 taatccttgc gttctgtaag ccggttcagg aggaaatcct gatccggcta ttccgaaagt
    11401 tatccgcccc cgtcctcctc ccccaaatat ccttcagatg atgagtgatc ctgcattagg
    11461 ctatggcaag gtgatcagat tttcatcaca ggggaattat gatgttaaat gcatggcacc
    11521 tgccggtgcc cccatttgtt aaacaaagca aagatcaact gctcattaca ctgtggctga
    11581 cgggcgaaga cccaccgcag cgcattatgc tgcgtacaga acacgataac gaagaaatgt
    11641 cagtaccaat gcataagcag cgcagtcagc cgcagccagg cgtcaccgca gggcttcagc
    11701 cgaatgccgc cggcacgact ggagcagttt gccgtcgatg taccggatat cggcccacaa
    11761 tgggctgcgg atcagatttt ttatcagatc ttccctgatc gttttgcgcg tagtcttcct
    11821 cgtgaagctg aacaggatca tgtctattac catcatgcag ccggacaaga gatcatcttg
    11881 cgtgactggg atgaaccggt cacggcgcag gcgggcggat caacgttcta tggcggcgat
    11941 ctggacggga taagcgaaaa actgccgtat ctgaaaaagc ttggcgtgac agcgctgtat
    12001 ctcaatccgg tgtttaaagc tcccagcgta catcaatacg ataccgagga ttatcgccat
    12061 gtcgatccgc agtttggcgg tgatggggcg ttgctgcgtt tgcgacacaa tacgcagcag
    12121 ctgggaatgc ggctggtgct ggacggcgtg tttaaccaca gtggcgattc ccatgcctgg
    12181 tttgacaggc acaatcgtgg cacgggggga gcttgttaca accctgaatc gccctggcgc
    12241 gactggtact cgtttagtga tgatggcacg gcgctcgact ggcttggcta tgccagcttg
    12301 ccgaagctgg attatcagtc ggaaagtctg gtgaatgaaa tttatcgcgg ggaagacagt
    12361 attgtccgcc attggctgac agcgccgtgg agtatggacg gctggcggct ggatgtggtg
    12421 catatgctgg gggaggcggg tggggcgcgc aataatatgc agcacgttgc tgggatcacc
    12481 gaagcggcga aagaaaccca gccggaagcg tatattgtcg gcgaacattt tggcgatgca
    12541 cggcaatggt tacaggccga tgtggaagat gccgccatga actatcgtgg cttcacattc
    12601 ccgttgtggg gatttcttgc caataccgat atctcttacg atccgcagca aattgatgcc
    12661 caaacctgta tggcctggat ggataattac cgcgcagggc tttctcatca acaacaatta
    12721 cgtatgttta atcagctcga cagccacgat actgcgcgat ttaaaacgct gctcggtcgg
    12781 gatattgcgc gcctgccgct ggcggtggtc tggctgttca cctggcctgg tgtaccgtgc
    12841 atttattacg gtgatgaagt gggactggat ggtaaaaacg atccgttttg ccgtaaaccg
    12901 ttcccctggc aggtggaaaa gcaggatacg gcgttattcg cgctgtaccg gcgaatgatt
    12961 gtgctgcgta agaaaagtca ggcgctgcgt cgtggcggct gtcaggtgct gtatgcggaa
    13021 gataacgtag tggtatttgt ccgcgtgctg aatcagcaac gtgtactggt ggcaatcaac
    13081 cgtggcgagg cctgtgaagt ggtgctaccc gcgtcaccgt ttctcaatgc cgtgcaatgg
    13141 caatgcaaag aagggcatgg gcaactgact gacgggattc tggctttgcc tgccatttcg
    13201 gctacggtat ggatgaacta acgttttata acgccttgtg tgacgcctgc gccatcatcc
    13261 gcggataaaa ctgccagaag cgggttccga gggcggtata atgggcgttt aaatcgtacc
    13321 aggagtcacg cagggcatcc agacgtgggc gacggcttgc catgccgttt aacacgttct
    13381 ggatgaaatc catatcgcga tagcgcacca gccactgctc tgaccacaag taattgttta
    13441 gattgataaa acgtggcggt gagtccggca aaatcgtcat cacttgcttg cgggcataac
    13501 agataaattc ctgtagcgga aagtccggcg acagctgcga ccagtggcgg gaaagaaagt
    13561 gatcccacat gacatccagc gtaataggcg caacgcggcg cgtttcacga cgaaaccact
    13621 cccgtgcttc gcggacttcc ggcagattgt cagtcaatac gtcgatacgt cgatgcatat
    13681 gaatgccagc cacgacgtcg ggcggaaaac tttcttcagg atttccgcgt acgaaatcag
    13741 ccagtaaatt gccggaaagc gagctttccg cgagatgggc taaatgcagg tgagctaaaa
    13801 aattcatcga ttatattcta tccaaagggg ggtaaaggtt gcagggagag cgccccggca
    13861 ctagactacc cgcctcttat tttagtctga gtcagtgtca tgcgcgttac cgatttcttc
    13921 tttgaattgc ccgaatccct gattgcccac taccccatgc ctgaacgcag tagctgtcgt
    13981 ttactgtcgc tggacgggcc tacgggcgcg ctgacgcacg gtactttcac cgatttactc
    14041 gataagctca accccggcga tcttctggtt tttaataata ctagcgtgat cccggcgcgc
    14101 ctgtttgggc gtaaagccag cggcggcaag attgaagtgc tggttgagcg gatgctcgac
    14161 gacaaacgca ttcttgcgca tattcgcgcc tcgaaagcgc caaaacctgg cgcagaactg
    14221 ctgctgggcg atgacgaaag tattaacgca acaatgaccg cgcgccacgg cgcactgttt
    14281 gaagtcgaat tcaatgatga tcgttcggta ctggatatcc tcaacagtat cgggcatatg
    14341 ccgctgccgc cgtatatcga ccgtccggac gaagacgctg accgcgaact ttatcaaacc
    14401 gtttatagcg aaaaaccggg cgcggttgca gccccgaccg ctggtctgca ttttgacgag
    14461 cctttgctgg aaaaattgcg cgccaaaggc gtggagatgg cgtttgtgac gttgcacgtt
    14521 ggtgcgggca ccttccagcc ggtgcgcgtc gacaccattg aagatcacat catgcactcg
    14581 gaatacgctg aagtaccgca ggatgtggta gacgcggtac tggcggcgaa agcgcgcggt
    14641 aaccgggtga ttgcggttgg caccacttca gtacgttcgc tggaaagcgc ggctcaggca
    14701 gcgaaaaacg atctcattga accgttcttc gacgataccc aaatctttat ctatccgggc
    14761 ttccagtaca aagtggtcga tgcgctggtg acgaacttcc acttgccaga gtcgacgctg
    14821 attatgctgg tttcggcctt tgccggttat caacacacca tgaacgccta taaagcagcg
    14881 gtagaagaga aatatcgctt ttttagttac ggtgatgcga tgtttattac gtacaatccg
    14941 caggcaatta atgagcgcgt cggggagtaa ttccgcggcg ctggtttaaa acgttggact
    15001 gtttttctga cgtagtggag aaaaaatgaa atttgaactg gacaccaccg acggtcgcgc
    15061 acgccgtggc cgcctggtct ttgatcgtgg cgtagtggaa acgccttgtt ttatgcctgt
    15121 tggcacctac ggcaccgtaa aagggatgac gccggaagaa gttgaagcca ctggcgcgca
    15181 aattatcctc ggcaacacct tccacctgtg gctgcgcccg ggccaggaaa tcatgaaact
    15241 gcacggcgat ctgcacgatt ttatgcagtg gaaggggccg atcctcaccg actccggggg
    15301 cttccaggtc ttcagccttg gcgatattcg taaaatcacc gaacagggcg tgcacttccg
    15361 taacccgatc aacggcgatc cgattttcct cgatcctgaa aaatcaatgg agattcagta
    15421 cgatcttggt tcggatatcg tcatgatctt tgatgagtgt acgccgtatc ctgctgactg
    15481 ggattacgca aaacgctcca tggagatgtc tctgcgttgg gcgaagcgta gccgtgagcg
    15541 ttttgacagt ctcggaaaca aaaatgcgct gtttggtatc attcagggca gcgtttacga
    15601 agatttacgt gatatttctg ttaaaggtct ggtagatatc ggttttgatg gctacgctgt
    15661 cggcggtctg gctgtgggtg agccgaaagc agatatgcac cgcattctgg agcatgtatg
    15721 cccgcaaatt ccggcagaca aaccgcgtta cctgatgggc gttggtaaac cagaagacct
    15781 ggttgaaggc gtacgtcgtg gtatcgatat gtttgactgc gtaatgccaa cccgcaacgc
    15841 ccgaaatggt catttgttcg tgaccgatgg cgtggtgaaa atccgcaatg cgaagtataa
    15901 gagcgatact ggcccactcg atcctgagtg tgattgctac acctgtcgca attattcacg
    15961 cgcttacttg catcatcttg accgttgcaa cgaaatatta ggcgcgcgac tcaacaccat
    16021 tcataacctt cgttactacc agcgtttgat ggcgggttta cgcaaggcta ttgaagaggg
    16081 taaattagag agcttcgtaa ctgattttta ccagcgtcag gggcgagaag taccaccttt
    16141 gaacgttgat taatattaat aatgagggaa atttaatgag cttttttatt tctgatgcgg
    16201 tagcggcaac gggtgcaccg gcgcaaggta gcccgatgtc tttgattttg atgctggtgg
    16261 tattcggtct gattttctat ttcatgatcc tgcgtccaca gcagaagcgc accaaagaac
    16321 acaaaaagct gatggactcc attgccaaag gtgatgaagt tctgacgaac ggtggcctgg
    16381 ttggtcgcgt aaccaaagta gcggaaaacg gctacattgc tatcgcgctg aatgacacca
    16441 ctgaagtagt tattaaacgt gacttcgtag ctgccgtcct gccgaaaggc accatgaagg
    16501 cgctgtaatt aaaatttttc cctaagggaa ttgccgtgtt aaaccgttat cctttgtgga
    16561 agtacgtcat gctgatcgtg gtgattgtca tcggtctgct gtatgcgctt cccaacctgt
    16621 ttggtgagga tccggctgtt cagatcactg gtgcgcgcgg agtcgccgcc agtgagcaaa
    16681 cgctgatcca ggtccagaaa acgttacaag aagaaaaaat aactgctaag tctgtggcac
    16741 tggaagaggg cgctattctt gcgcgcttcg actccactga cacccagctg cgcgctcgtg
    16801 aagcattaat gggcgttatg ggtgacaaat acgtcgtggc gcttaacctt gccccggcaa
    16861 cgccgcgctg gctggcagct attcacgctg agccgatgaa gctcggcctt gacctgcgtg
    16921 gcggcgttca cttcctgatg gaagtggata tggacaccgc gcttggcaaa ctccaggaac
    16981 aaaatatcga tagcctgcgc agcgatctgc gtgaaaaggg catcccgtat accaccgttc
    17041 gtaaagaaaa caactacggc ctgagcatta ctttccgcga tgctaaagct cgtgatgaag
    17101 ccattgcgta tctgagcaag cgccatccgg acctggtgat tagtagccag ggcagcaacc
    17161 agctgcgtgc ggtaatgagc gatgctcgtc tgagtgaagc gcgtgaatat gcggtgcagc
    17221 agaacatcaa tatcctgcgt aaccgtgtaa accaacttgg cgtagcggag ccggtggttc
    17281 agcgtcaggg tgctgaccgt atcgttgttg aactgccagg tattcaggac actgcgcgtg
    17341 cgaaagagat tctgggtgcg acggcaacgc tggaattccg tctggtaaac accaacgttg
    17401 accaggccgc tgcggcatcc ggtcgcgtac cgggcgactc tgaagtgaaa cagacccgcg
    17461 aaggtcagcc agttgtgctg tacaaacgcg taattctgac cggtgaccat atcaccgact
    17521 ccacttccag ccaggacgaa tacaaccaac cgcaggttaa catctcgctc gatagcgctg
    17581 gtggtaacat catgtctaac ttcactaagg acaacatcgg caaaccgatg gcaaccctgt
    17641 ttgtggagta caaagacagc ggtaagaaag atgcaaatgg tcgtgcggtt ctggtgaaac
    17701 aggaagaggt gattaacatc gccaacatcc agtctcgtct gggtaacagc ttccgtatca
    17761 ccggcatcaa caacccgaac gaagcccgtc agctgtcact gctgctgcgt gccggtgcgt
    17821 tgatcgcgcc gattcagatt gttgaagaac gtaccattgg cccaaccctg ggtatgcaga
    17881 acattgaaca ggggctggag gcttgccttg ccggtctgct ggtttctatt ctgttcatga
    17941 tcatcttcta taagaagttt ggtctgattg cgaccagtgc tctgattgcc aacttgatct
    18001 taatcgtcgg cattatgtcg ctgttgccag gcgcaacgct gagtatgcca ggtatcgcgg
    18061 gtatcgtctt aacccttgcg gtggcggtcg atgcgaacgt actgatcaac gaacgtatta
    18121 aagaagagtt gagcaacgga cgtactgttc aacaggcaat tgatgaaggt tatcgtggcg
    18181 catttagttc tatcttcgat gcgaacatca ccacgctgat taaagtcatc atcctgtacg
    18241 cagtgggtac cggggcaatt aaagggttcg cgattactac cggtatcggt gtggcgacgt
    18301 cgatgtttac cgcgattgtc ggtacgcgtg ccatcgtaaa cctgctatat ggcggcaagc
    18361 gcgtcaagaa gctgtcaatc tgaggagtgc gatgtggcac aggaatatac tgttgaacaa
    18421 ctaaaccacg gccgtaaagt ctatgacttt atgcgctggg actactgggc tttcggcatc
    18481 tctggtctgc tgttaatcgc cgctatcgtt attatgggcg tgcgcggctt taactggggg
    18541 ctggatttca ccggtggtac ggttatcgaa attacgctcg aaaaaccggc tgaaattgac
    18601 gtaatgcgtg atgcattgca aaaagccggt tttgaagagc caatgctgca aaactttggt
    18661 agcagccacg acatcatggt ccgtatgccg cctgctgaag gcgaaaccgg cggtcaggtg
    18721 ctgggcagcc aggttctgaa ggtgattaac gaatccacca atcagaatgc agcagtgaag
    18781 cgtattgagt tcgtcggtcc gagcgtgggg gaagacctta cgcaaaccgg tgcgatggcg
    18841 ttgatggcgg cgctgctgtc tatccttgtg tacgtaggtt ttcgctttga gtggcgactg
    18901 gcggcagggg ttgttattgc gctggcgcac gacgttatca ttacgctggg tattttgtcg
    18961 ttattccata tcgagattga cctgaccatt gtggcatcgt tgatgtcggt tatcggttac
    19021 tcgcttaacg acagtatcgt ggtatcggac cgtattcgtg aaaacttccg caagatccgt
    19081 cgcggtacgc cttacgaaat ctttaacgtg tccttgaccc agacgctgca ccgtaccttg
    19141 atcacatccg gtactacctt gatggttatc ctgatgctgt acctcttcgg tggtccggta
    19201 ctggaaggct tctcgctgac catgcttatc ggtgtttcca tcggtactgc atcttccatc
    19261 tatgtggcat ctgcgttggc tctgaaactg ggtatgaagc gcgaacacat gttgcagcag
    19321 aaagtggaaa aagaaggggc ggatcagccg tcaattctgc cgtaatcaag ttcccgttga
    19381 tgttgaaaat cccggtcaga agatcgggat tttttttgat gtatggagat tgcgaaaatc
    19441 cccgcatctt gggaaactgc gcgtaaccct acatttcatc caggtaactc ttcatggcta
    19501 tcatcccaaa aaactatgcg cggttagaaa gcggctatcg cgaaaaagca ttaaaaatat
    19561 atccgtgggt ctgcggtcgc tgttcccgcg agtttgttta ttccaacctg cgtgaactta
    19621 ccgttcacca cattgatcac gaccatacca ataacccgga agatggcagt aactgggaat
    19681 tgctttgtct ctattgccac gatcatgagc attcgaaata taccgaagcg gatcagtatg
    19741 gtacgaccgt tatcgcaggg gaagatgcgc agaaagatgt cggtgaagcg aagtacaacc
    19801 cattcgctga cctgaaagcg atgatgaaca agaagaagtg attaaaacgt aaaattgcct
    19861 gatgcgctac gcttatcagg cctacgttat ttcagcaata tattgaattt acgtgctttt
    19921 gtgggccgga caaagcgttt acgccgcatc cggcatgaac aaagctcacg ttgttaacaa
    19981 tcagaaatgc cgggaataaa tcccggcatt ttcataatca gaagttgtaa cctactacca
    20041 ggtaaccacc ccagccggta gagcgaacgt tgaagttgcc gttgccgaag ttcagttctg
    20101 catcgtcgtt ccactgacca ccgtcgtgcc agtaacgagc tacgacagag tagtgccagt
    20161 gatcgtagtt cagagccaga atatggctgg aagcgataga gttattagta cgggtcttaa
    20221 taccgttgat tgcgttaccg ctgtcatccc ctaaatcgga accccagtcg aagttggtga
    20281 agccgatgta gctcagctga ccgccccaca gatcggtaat cggcacaaag tatttaattt
    20341 tgaaacggta accgtcccac tcgttttcgt tcgctgcgcc atagttctgc cactggtatt
    20401 tcgcatagac gttcatggac aggctcatcg gcagatcagt atcgatgtcg gtacccagac
    20461 ccatgtacca ggtgctctgg cgaccatttt tattacgacc catgtcgtaa atgtagttgt
    20521 tcgcgaagta ccactctttg aacggaccga agttaaggtc agtattggtc agcttgtcga
    20581 tggagaaacg tggttcgatt tccataaaca gcggagaacc gtggttccag atacctttag
    20641 catcggagtt accgccgaag aataccggcg catccgcata accatagaag tcgaaccagt
    20701 cttttttagc gaatgcttcg tactcaaggt aggtatcgtt gcggatctgc ggtccgaaac
    20761 gggtgtgata gcttccgaca acgttaacgc tctggtgcca ccagtcggaa agatactgcg
    20821 gtttgtcgtt ttcagctgcg ttgacagtaa aagacgaaga gagcgccagt accgcaccgg
    20881 ctgccagtaa tgtttttttc atatgtatgc cactgtttga aaatcccttg cgggagtgaa
    20941 aaaggcgcaa attgcgtttc taaatatttc gtatacagag gagcctatta taaaaatcat
    21001 tgctcacaaa aatatgtttc gtttcacagt tctatcattt acgtaatcga ttgcgttcac
    21061 gtttacacac attcgggcgg ggattgtact gactttcgcc cttgttgcaa acttttacta
    21121 tcaatccagg tgatgtaagc agagtaacaa aatgacgggg aaagatgttg cgggacgcac
    21181 aaaatcgtcc cgaaatacgt ttactgcata acaggttgaa tgtcgtggat acgtacaaag
    21241 cctaactgat ccacagaaat cccctttaat tgtaacggaa tatcaacatc gctgggggcg
    21301 aggacgctgg caggggcatt aatcaattga ttctgcacat tgatttcctg aaagttgtct
    21361 attgttccct gaatttgccc atattcaaca gttccgctga atgcgggcaa gggatcattc
    21421 gactcaccct gaatacgtag tgtaagggtg gtgccatctg tatcaggcgt aatattcacc
    21481 agcgacatac gtaaagtacc gatctggctt tccagtcttg ccggtgtttt cgctctaggc
    21541 agaaggtaac ccccactgct ggattttgca ttcagcctgt tttgctgagt gattttgact
    21601 gtttcttgat tgagttgcgt catctcctgg ttcagcgtgc tgacgctgtg tttcatctgt
    21661 cgcacttcac tttgctgcac acaggcgcta aggctgaaca ggcttcccag caggagcagt
    21721 cgaaaaacgt tagtattcat aggtcgtccc tttctcgtcg caattagcct aatggtagag
    21781 ggtaaaagca gcgaaagcat cgatccttta tctcaaaagc gttgcgcctt tgttgtatcg
    21841 tcagttcagg gtaaaataga tttccgttaa ccacttggtc aggacgccgt atgcattgcc
    21901 cattctgttt cgccgtggac actaaggtaa ttgactctcg tctcgtgggc gagggttcat
    21961 ccgtacgccg ccgtcggcag tgtctggtgt gtaatgaacg tttcaccacc tttgaagtgg
    22021 cggagctggt tatgccgcgt gttgtaaaaa gcaacgacgt gcgtgaaccg tttaatgaag
    22081 agaaattgcg tagcggaatg ctgcgggcgc tggaaaaacg tccggtgagt tccgatgacg
    22141 ttgaaatggc aatcaatcat attaaatcgc agctgcgcgc caccggtgag cgcgaagtac
    22201 cgagcaagat gattggcaat ctggtgatgg agcaattgaa aaagctcgat aaagtcgcct
    22261 atatccgttt tgcctctgtc taccgcagtt tcgaagatat caaagaattt ggcgaagaga
    22321 tcgcgcgcct ggaggactaa gccgtgcagg acgagtatta catggcgcgg gcgctaaagc
    22381 tggcgcaacg aggacgtttt accacccacc ccaacccaaa tgtcgggtgc gtcattgtca
    22441 aagatggcga aattgtcggt gaaggttacc atcaacgagc gggtgaacca catgccgaag
    22501 tacacgcgtt gcgtatggcg ggtgaaaaag ccaaaggtgc aacggcgtat gtgacactcg
    22561 aaccttgcag ccatcacggc cgtacgccgc cttgctgtga tgctttaatt gccgcaggtg
    22621 tggcgcgcgt ggttgcctcg atgcaagatc ctaacccgca ggtcgctggg cgtggacttt
    22681 accgtctgca acaggctggc attgacgtca gccacggcct gatgatgagt gaagccgagc
    22741 aattgaataa aggctttctc aagcggatgc gcaccggctt tccttatatt cagttaaaac
    22801 ttggcgcatc gcttgatggt cgcacggcga tggcgagcgg cgaaagccag tggatcactt
    22861 cgccccaggc gcggcgtgat gtacaacgac tgcgcgcgca aagtcatgcc attttaacca
    22921 gcagcgccac ggtgctggcg gatgatcctg ccttaacggt gcgttggtcc gaactggatg
    22981 aacaaactca ggcgctctac ccgcaacaaa atctccgcca gccggtacgt attgtgattg
    23041 atagccaaaa tcgcgtgacg ccggaacatc acattgtgca gcagcccggc gaaacctggt
    23101 tcgcgcgtac gcaggaagat tctcgtgagt ggccggaaac ggtgcgtacc ttgctgattc
    23161 cagagcataa aggtcatctg gatctggttg tactgatgat gcaactgggt aaacagcaga
    23221 ttaacagcat ctggggggaa gcggggccaa cgctcgctgg cgcattgctg caggcgggtt
    23281 tagtcgatga gctgattgtc tatatcgcac ctaaactatt aggcagcgac gcccgtggat
    23341 tatgcaccct gccagggctt gagaaattag ccgacgcccc ccaatttaaa ttcaaagaga
    23401 tacgtcatgt aggcccggat gtttgcctgc atttagtggg tgcatgatct cccggctcga
    23461 aagggaagca gcgcacgaaa tattatgcta aaatccgccc ccctgcgggg ccatactcga
    23521 acccgaagga agaaaatgaa cattattgaa gctaacgttg ctaccccgga cgctcgcgtc
    23581 gccatcacca tcgcgcgttt caacaacttt atcaatgaca gcctgctgga aggtgcaatt
    23641 gacgcactga aacgtatcgg tcaggtaaaa gatgaaaaca ttaccgttgt ttgggtgcct
    23701 ggtgcctatg agctgccgct ggcggcgggt gcactggcta aaaccggtaa atacgacgcg
    23761 gtgattgcgc tgggtacggt tattcgtggt ggcactgccc attttgaata tgtcgctggt
    23821 ggtgcaagca acggcctggc gcatgttgcc caggacagcg aaattccggt tgcttttggg
    23881 gttctgacca ctgaaagcat tgaacaagcg atcgaacgtg ctggcaccaa agctggcaac
    23941 aaaggtgcag aagctgcact gaccgcgctt gaaatgatta atgtattgaa agccatcaag
    24001 gcctgaaatt agtaagggga aatccgtgaa acctgctgct cgtcgccgcg ctcgtgagtg
    24061 tgccgtccag gcgctctact cctggcagtt gtcccagaac gacatcgctg atgttgaata
    24121 ccagttcctg gctgaacagg atgtaaaaga cgttgacgtc ctgtacttcc gtgagctgct
    24181 ggccggggtg gcgactaata ccgcatacct cgacggactg atgaagccat acctgtcccg
    24241 tctgctggaa gaactgggac aggtagaaaa agcagtactg cgcattgcgc tgtacgaact
    24301 gtctaaacgt agcgatgtgc catacaaagt ggccattaac gaagcgatcg aactggcgaa
    24361 atcgttcggc gcagaagaca gccataagtt cgtcaacggt gtactcgata aagcagcacc
    24421 tgtgattcgc cctaacaaaa agtaatatcc aggccggtag attcacggaa gaccgttcca
    24481 tgatcgccgg ccttttcttt tttacctgct gaggcataac gtatggcatg tggcgagttc
    24541 tccctgattg cccgttattt tgaccgtgta agaagttctc gtcttgatgt cgaactgggc
    24601 atcggcgacg attgcgcact tctcaatatc cccgagaaac agaccctggc gatcagcact
    24661 gatacgctgg tggcgggcaa ccacttcctc cctgatatcg atcctgctga tctggcgtat
    24721 aaagcactgg cggtgaacct aagcgatctg gcagcgatgg gggccgatcc ggcctggctg
    24781 acgctggcat taaccttgcc ggacgtagac gaagcgtggc ttgagtcctt cagcgacagt
    24841 ttgtttgatc ttctcaatta ttacgatatg caactcattg gcggcgatac cacgcgtggg
    24901 ccattatcaa tgacgttggg tatccacggc tttgttccga tgggacgagc cttaacgcgc
    24961 tctggggcga aaccgggtga ctggatctat gtgaccggta caccgggcga tagcgccgcc
    25021 gggctggcga ttttgcaaaa ccgtttgcag gttgccgatg ctaaagatgc ggactacttg
    25081 atcaaacgtc atctccgtcc atcgccgcgt attttacagg ggcaggcact gcgcgatctg
    25141 gcaaattcag ctatcgatct ctctgacggt ctgatttccg atctcgggca tatcgtgaaa
    25201 gccagcgact gcggcgtacg tattgacctg gcattgctgc cgttttctga tgcgctttct
    25261 cgccatgttg aaccggaaca ggcgctgcgc tgggcgctct ctggcggtga agattacgag
    25321 ttgtgtttca cggtgccgga actgaaccgt ggagcgctgg atgtggctct cggacacctg
    25381 ggcgtaccgt ttacctgtat cgggcaaatg accgccgata tcgaagggct ttgttttatt
    25441 cgtgacggtg aacctgttac gttagactgg aaaggatatg accattttgc cacgccataa
    25501 agatgtcgcg aaaagtcgcc tgaagatgag taatccgtgg catctacttg ctgtcggatt
    25561 cggaagtgga ttaagcccga tcgttcctgg gacgatgggc tcgctggcag cgattccgtt
    25621 ctggtatctg atgacctttt tgccctggca gctctactcg ctggtggtga tgctggggat
    25681 ctgtatcggc gtctatcttt gtcatcagac cgcaaaagat atgggcgtgc acgatcacgg
    25741 cagcattgtc tgggacgaat ttattggtat gtggatcacg ctcatggcgc tgccgaccaa
    25801 tgactggcag tgggttgccg ccgggtttgt gattttccgt attctggata tgtggaaacc
    25861 gtggccgatc cgctggtttg atcgcaatgt gcatggcggc atggggatca tgatagatga
    25921 tattgtcgcc ggggtgatct ccgctggcat cctgtacttt attggtcatc actggccgct
    25981 gggcattctg tcgtagttgt gcaccgatgc ctgatgtgac gcttgttgcg tctcatcagg
    26041 cctggactct tatttaaatc ctactaccgg atgcggttta tacggcgttt ccagttcggc
    26101 aatctgttcc ggcttcaaag tgatatccac cgcgttcaat agctcatcaa gctgttcttc
    26161 ccgcgatgta ccgataatcg gtgcggcaat gcccggttta ctcaacaacc aagccagcgc
    26221 aacttgtgct cgtgttgcac cgagttcttc actgacgccc gttaaccgct ctgcgatctg
    26281 cgcgtcattt tcatcgcttt ctttatagag atttttcccc acctcatcag acaccagtcg
    26341 tgcggtagtt tctccccacg gacgcgtcag tcgcccccgc gccagcgggc tccacggaat
    26401 caccgccacg ccctcctgat aacacagtgg cagcatctcg cgctcttctt cgcgataaat
    26461 cagattgtag tgatcctgca tactgacaaa ctgcgcccag ccgtgctgtt tttggagttc
    26521 cagtgcctga gcaaactgcg aagcgtgcat tgatgacgcg ccgatataac gcgctttccc
    26581 ggcttttacc acgtcgttca gggcttccag cgtctcttcg atcggcgtgt tgtaatccca
    26641 gcgatgaatt tgcaggatat cgacataatc catgccgaga cgtcgcaggc tgtcgtcgat
    26701 agagcgcaaa atttgtgcac gggataatcc ttccggtaaa tcaccaacgc gatggaacac
    26761 tttggtggca acgaccacgt cttcacgacg ggcgaaatcc cgcagtgcgc gaccaacgat
    26821 ctcttcgctg ctaccatcgg aatagctatt ggcggtatca aagaaattta tgccgccttc
    26881 cagtgcgcgt ttaattatgg gacggctgct ctcttccggc agtgtccatg cgtgattacc
    26941 acgatctggc tcgccaaagg tcatacagcc gaggcaaagt cgggaaacgc gaaggtcggt
    27001 ttttcctaag gggttgtatt gcatgctgcc actcctgcca tgaataattt cttaagcata
    27061 gcaggagtgg agtagggatt atgccagcca ggccttgatt ttggcttcca taccggcggc
    27121 atcgaggccg agttcggcgc gcatttcttc ctgcgttcct tgcggaataa agaagtcagg
    27181 caggccaatg ttcagcacgg ggactggttt acgatgggcc attagcactt cgttcactcc
    27241 gctgcctgca ccgcccataa tggcgttttc ttctacggta accagtgctt catgactggc
    27301 ggccatttcc agaattaacg tttcatcaag cggtttcaca aaacgcatat cgaccagcgt
    27361 ggcgttcagc gattcggcga ctttcgccgc ttctggcatc agcgtaccaa agttaaggat
    27421 cgccagtttc tcgccacgac gcttcacaat gcctttgcca attggtagtt tttccagcgg
    27481 cgtcagttcc acgccgaccg cgttgccacg cgggtagcgc accgctgacg ggccatcgtt
    27541 atagtgatag ccggtataga gcatctggcg acattcgttt tcatcgctcg gggtcataat
    27601 gaccatttcc ggtatgcagc gcaggtaaga gagatcaaaa gcaccctgat gggtttgacc
    27661 gtcagcacca acaatgcccg cgcggtcgat ggcgaacagg accggaagct tttgaattgc
    27721 cacgtcatgc agcacctgat cataggcgcg ttgcaggaaa gtggagtaga tcgcgacaat
    27781 gggtttgtac ccaccaatcg ccagaccggc ggcaaaggtc actgcgtgtt gctcggcaat
    27841 tgccacgtcg aagtagcgat ccgggaattt acgagaaaac tcgaccatgc cggaaccttc
    27901 acgcatcgcc ggagtaatcg ccatcagctt gttatctttc gccgccgttt cgcacaacca
    27961 gtcgccaaag atttttgaat agctcggtaa accgccgcta cttttcggca aacaaccgct
    28021 ggagggatca aatttaggca cggcgtggaa agtgattggg tctttttctg ccggttcata
    28081 accacgacct tttttggtca tgatatgcag gaactgcggg cctttcaggt cacgcatgtt
    28141 cttcagcgtg gtgataagcc ccagcacatc gtgaccgtca accgggccga tgtagttaaa
    28201 gcccagctct tcaaacaacg tgccagggac taccatgcct ttaatatgtt cttcggtacg
    28261 tttgagcagc tctttaatgg gtggcacgcc agagaaaact tttttcccgc cttcgcgcag
    28321 tgaagagtaa agcttaccgg aaagcagctg cgccagatgg ttgttgagcg cgccgacatt
    28381 ttcggaaatc gacatttcgt tgtcgttgag gaccaccagc atatcaggac ggatatcgcc
    28441 cgcgtgattc atcgcttcaa acgccatgcc tgcggtaatc gcgccatcgc cgattacaca
    28501 gacggtacgg cgatttttgc cttctttctc ggcagcaacc gcaataccaa ttccggcact
    28561 gatggaggtt gatgaatgcc cgacgcttaa tacgtcatat tcgctttcgc cgcgccacgg
    28621 gaacgggtgc aggccgcctt tctgacggat ggtgccgatt ttgtcgcggc gtccggtcaa
    28681 aattttatgc ggataagcct gatgccccac atcccaaatc aattggtcaa acggggtgtt
    28741 gtagacatag tgcagcgcca cggtcagttc gaccgtgccc agcccggagg cgaagtgccc
    28801 gctggaacgg ctcacgctgt cgagtaaata gcggcgcagt tcgtcgcaga gtttcggtaa
    28861 actctctttc ggcaacagtc gtaactcctg ggtggagtcg accagtgcca gggtcgggta
    28921 tttggcaata tcaaaactca tcaggggcct attaatactt attgtttatt tattacgctg
    28981 gatgatgtag tccgctagcg cttccagtgc cgaggtatcg ggtgactgtt cagccagttg
    29041 tttcagcgac tgacgggcat cgtcgatcag atcccgggct ttcttccggg cttgctcaag
    29101 acccagaagt gcagggtagg tacttttacc aagttgctgg tcggcaccct ggcgttttcc
    29161 caacgttgca gtatctccta ccacatccag gatgtcatcc tgaacctgga aggcaaggcc
    29221 gatgctctct gcgtacttgt cgagtactgg cagagcacga cgccctttat ctccggcgct
    29281 taatgcacca aggcgaacgg cggcgcgaat caatgcgccg gttttatgac gatgaatacg
    29341 ctcaagcgcg tccagaggta cgtgtttgcc ttccgcgtct aaatctaatg cctgaccacc
    29401 gcacattccg gcaataccgc tggcgctcgc cagttcagaa atcatcgaaa ttctgtcgcg
    29461 atccgacact tccggcatat cagcatcgct taaaatcgag aacgccagcg tttgtaaagc
    29521 gtcgccagcg agaatcgcgt ttgcttcgcc aaacttcaca tggcaggtcg gcaaaccgcg
    29581 acgcagatcg tcatcatcca tcgccggtaa atcatcatga attaatgagt aagcgtggat
    29641 acactctacg gcggcagcgg gtgcgtccag cgtgtttgtg ctaacgccaa acatatgacc
    29701 ggtggcataa accaggaagg gtcgcagacg cttaccacct aataatgcac catactgcat
    29761 ggtttcgacc acgggagtgt tctgaaaggg cagtggggcg ataaaacggc tcagcgcctg
    29821 gttggcctgc ttaacgcagg cttcgagttg ctgcggaaag tccattactc attgtccggt
    29881 gtaaaagggg ttagagaggc gtcttcattg tcagacagca gaatttgtac gcgctgttcg
    29941 gcttgttgta atttggcctg cccctgacgt gccagctgca cgccgcgttc gaactcgttc
    30001 agcgcctctt ccagcggcag gtcgccactt tccagacggg ttacaatctg ttccagctcg
    30061 ctcagcgcct tttcaaagct ggcgggagcc tcatttttct tcggcataat gaatgtctga
    30121 ctctcaatat tttttgcccc gtcatggtaa cggactcagg gcaaatagca aataacgcgc
    30181 aatggtaagg tgatgtgcgc agcaaagcga tgttagtggt atacttccgc gcctggatgc
    30241 agccgcaggt gtgggctgct gtatttttcc ctatacaagt cgcttaaggc ttgccaacga
    30301 accattgccg ccatgaagtt tatcattaaa ttgttcccgg aaatcaccat caaaagccaa
    30361 tctgtgcgct tgcgctttat aaaaatcctt accgggaaca ttcgtaacgt tttaaagcac
    30421 tatgatgaga cgctcgctgt cgtccgccac tgggataaca tcgaagttcg tgcaaaagat
    30481 gaaaaccagc gtctggctat tcgcgacgcc ctgacccgta ttccgggtat ccaccatatt
    30541 ctcgaagtcg aagacgtgcc gtttaccgac atgcacgata tttttgagaa agcgttggtt
    30601 cagtatcgcg atcagctgga aggcaaaacc ttctgcgtac gcgtgaagcg ccgtggcaaa
    30661 catgatttta gctcgattga tgtggaacgt tacgtcggcg gcggtttaaa tcagcatatt
    30721 gagtccgcgc gcgtgaagct gaccaatccg gaagtgactg tccatctgga agtggaagac
    30781 gatcgtcttc tgctgattaa aggccgctac gaaggtattg gcggtttccc gatcggcacc
    30841 caggaagatg tgctgtcgct catttccggt ggtttcgact ccggtgtttc cagttatatg
    30901 ttgatgcgtc gcggctgccg cgtgcattac tgtttcttta accttggcgg cgcggcgcat
    30961 gaaattggcg tgcgtcaggt ggcgcattat ctgtggaacc gttttggcag ctcccaccgc
    31021 gtgcgttttg tcgctattaa tttcgaaccg gtcgtcgggg aaattctcga gaaaatcgac
    31081 gacggtcaga tgggcgttat cctcaaacgt atgatggtgc gtgccgcgtc taaagtggct
    31141 gaacgttacg gcgtacaggc gctggtcacc ggcgaagcgc tcggccaggt gtccagtcag
    31201 acgctgacca acctgcgcct gattgataac gtctccgata cgctgatcct gcgtccgctg
    31261 atttcttacg acaaagagca catcatcaac ctagctcgcc agattggcac cgaagacttt
    31321 gctcgcacga tgccggaata ttgcggtgtt atctccaaaa gcccgacggt gaaagcggtt
    31381 aagtcgaaga ttgaagcgga agaagagaaa ttcgacttca gcattctcga taaagtggtt
    31441 gaagaagcga ataacgttga tatccgcgaa atcgccctgc agaccgagca ggaagtggtg
    31501 gaagtggaaa ccgtcaatgg cttcggcccg aacgacgtga tcctcgatat ccgttctatc
    31561 gatgaacagg aagataagcc actgaaagtc gaagggattg atgtagtttc tctgccgttc
    31621 tataaactga gcaccaagtt tggcgatctc gaccagaaca gaacctggct actgtggtgt
    31681 gagcgcgggg tgatgagccg tctgcaggcg ctctatctgc gcgagcaggg ctttaacaat
    31741 gtgaaggtat atcgcccgta atttgtggtt gtaacgtcgc atctggtcag atgcgacgct
    31801 taccgcatcc gacactactc gtaataatta taaatccctg ccgccatcac cagttgtgat
    31861 gccacttcat gggctttctc gcgcccaacc tgcaggtcga taattttcag accaaagtcg
    31921 atagctgtcc ccggcccctg gctggtcagc aattttaccc gtgcatccca gacgacgcgc
    31981 ttgtcctgcc attgttcagc gggaattttg tctttcagcg tcgggaagcc ggtcatatta
    32041 ccaatcggga agatatcgtg cggcaccagc acggtggctg gcgcggcgca aatagccgcg
    32101 acgatacgcc cggaacggtg gaactgttta acggtttcaa ccagcagagt gctatcgcga
    32161 aaacactctg cgcctttaat gccaccaggc agtacgatca cgtcatattc gccatcagcc
    32221 acctcgacca gcggcgcatc cgccagcagc ttcacgccgc gcgagcaggt aattgccagg
    32281 ttgccatcgc tggctacgct ggcagtggtg actttgatac cgccgcgaac caagaggtcg
    32341 atcgtggtga cggcttcagt ctcttcacta ccaggggcga ggcaaaccag tgccgatgcg
    32401 ctcatattca ctctcctttc tttttaccat ttcaaacagg cgggtgtttt ccggtacggc
    32461 aatcccatgc gcgcgggcgc ggcgtaagag aaaaccattg atatagtcga tttcggtgtg
    32521 gcgcagcgcg cggatatcct gcaacatcga cgagatattt tccgctgtgg catcaatcac
    32581 ctgcatcacg taatcacgca aatcttccgc tgaagtatga tgcccttcgc gttcgatcac
    32641 tgccgcgact tcttcgcata tttgcataat ttcttgcgga tgatgacgta attcaccgtt
    32701 cgggcaattc cagatggcgg tcagtggatt aatcacgcag ttgactgcca gcttgcgcca
    32761 cagctcggcg cgaatattgt tatgccaggc aacgtcaggc aacacggttt gtaaaatatc
    32821 cgccagataa ctgtaatcgc catcctgttg ccgtgccgga ccaatatgcg tgataccgtt
    32881 tgccacatga ataatgacat tgccgtcgcg gcgggcggca tgggtggtgg tgcccatcag
    32941 taatggctgc tgaatatttt gcaactcttc gatggtgccc atgccgttgt gaattaacag
    33001 tattggcgtg gttacaggca gtgtggacgc gaggcttttg acggcatcgg aaacctgcca
    33061 tgctttcagc gtcaccagga gcagatcgct ggtggcgaga aaatcgggat cgttggcggt
    33121 cagcgattcg ttaaatatcg aaccatctgt ctcaaccaga ttcgcactac aataaggttg
    33181 cggtacgcgc agccagccct gaacttcatg accctgtttg caaagtgctg taagccataa
    33241 ttgccctaag gcaccgcatc ccaatacggt aattttcatt gttcctcctc acccgcaacc
    33301 actccgggtg ttcaataagg ctatccctta attgtgcatg ctgttgcgac tatgcacaat
    33361 taagggatac gtcctggtgc aggactgtcg gttatttaac tttgcgggta ttatgcttcg
    33421 catcaaaaat gaagggagag gaaaagatgc catctttcga tattgtctct gaagttgatc
    33481 ttcaggaagc acgtaacgcg gtcgataacg cgagccgcga agtggagtcc cgttttgact
    33541 tccgtaacgt tgaagcctca tttgagctga acgacgccag caaaaccatc aaagtgttga
    33601 gcgagtccga tttccaggtc aatcagttgc tggatattct gcgtgccaag ctgctgaagc
    33661 gcggcattga aggcagttca ctggatgtac cggaaaatat cgttcatagc ggtaaaacct
    33721 ggtttgtgga agcgaaactg aagcagggca ttgagagcgc gacccagaaa aaaatcgtca
    33781 agatgatcaa agacagcaaa ctgaaagtgc aggcgcaaat tcagggcgat gagatccgcg
    33841 tgactggcaa atctcgtgat gatttgcagg ctgtcatggc gatggtgcgc ggtggcgatc
    33901 tgggtcagcc gttccagttc aaaaacttcc gcgattaatc gcgacaacct gcgctttgtt
    33961 catgtcagat gcggcatgaa cgcctggttc ggcctacaaa ggcttgtaaa tcaggagctt
    34021 agtaggcctg atgagcgtag cgcagcaggc aatttcgcct cgcttttatg cctggcgaat
    34081 tgcctgttca acatcaaagc gattcgtcac tttgctgtcg attttcacat aagctgaatg
    34141 ttcttcttct gcaatcaaca cttctttgat gccttcagtt tctagcaaac gcacttttaa
    34201 cgcctcgttt gcggcaatgt tcgccgggat ttcaatgcgc aaactgctga catacgccgg
    34261 ttctttcatg gtactggcga ctgccagcca cactgcggcc agcattgcgc cagcgagaaa
    34321 tacgccctga ccgtcaaaca tgccgtcaat ccagccgccc agtgaaccgc caatcgccac
    34381 gccaagaaac tggctggtgg agtaaacacc catcgctgta cctttgtaac ctgctggcga
    34441 ctctttactg ataagtgaag gcagaagggc ttccatcaaa ttaaacgcta caaagaaaag
    34501 ctgcacgccg accaccagtt gccagaactg cgtttgcgcg ttccacaaca caatttccgc
    34561 aaccacgatc aatccgacgc agaagacaaa gacttgcttc attttgcgct taacttcagc
    34621 gtagataatg aaaggcacga ccgagccaaa ggcgattagc attgtcgcca gatagacctt
    34681 ccagtgttca gccgccggga accctgcatc agccagttgt ccgggcaggg caacaaacgt
    34741 cgacatcagc aaaatatgca gacacataat gccaaagttg agtttcagca gccgcggttc
    34801 cgccagcact ttactgaaac tgcctttcac cattccggac tcacgattaa gtacgtgagt
    34861 gctactgttg ggcacaaccc aaatggtcaa cgcaatgccg gtcgttgcca gaatagcgat
    34921 catccagaac agcgcgtgca gcccaagttt gtgagtgatg atcgggccaa gcaccatcgc
    34981 aatggcaaag gtaatgccaa agctcacgcc gataaacgcc attgctttgg tgcggttttg
    35041 ttcgcgcgtg agatcggaaa gcagcgccat aacggcagcg gcaatcgcac cggagccttg
    35101 tagcgcccgg cctagaataa ttccccagat ggagtcagag agcgcagcga taacgctacc
    35161 ggcggcaaac accgccagcc caccgacaat taatggtttg cgaccaatac ggtcggaaag
    35221 caggccaaac ggaatctgaa aaacggcctg agtcagacca taaataccaa tggcaatacc
    35281 gattaatgct tcgctggcac cttgcagagc catgccatac gtggtcagaa ccggcagaac
    35341 cataaacatg cccagcatgc gcaacgagaa tacggtccct aaaccccagg tcgcgcgcct
    35401 ctcacctggc gtcattttat aatcgttcat taccacctct gttttaaatt cgcgactagt
    35461 gtaaagcggc aaacgaatgg ggtaaacatg cggatttttt agtaaatatt tcgagaggga
    35521 tatgtttcta atgctaagaa aaaaggtgcc gtagcacctt tttaatagag aggttttgtt
    35581 accacacagc agccagcagc gtatgcgagt ccggtaccat aaaatcaacg gacatcatca
    35641 ccgagagggc agtgatggcg atgatagaga agccgaacag cttgcgcgcc cagattctgt
    35701 catcagcaac tttataaccg cgcagagcca tacctaacca ccagacgcta accgccgcgg
    35761 cgaccaccag atatttatat ccagcgtaac cgccaagaga gagcatcagc gtggcaacgg
    35821 caaaggcgat gatatacagc gtgatgtgat tcttcgccac cgaaatgcct tttaccactg
    35881 gcaataccgg aatgtttgcc gcctggtaat ccttaaagcg gaaaatggcg atggcatagg
    35941 agtgaggcat ctgccacagg ctgaagatag ccagcaggat cgctgcgccg ctatcgaact
    36001 cgccggttac tgcacagtag ccaatcaccg gcggcgcagc gccggagagc gaaccaatca
    36061 atgtgccgta gactgagtgg cgtttcatgt acaggctata gacgccgaca taaaccacaa
    36121 agcccatcac ccccagccag caggccagcg gattcgcgcc aaaccacagc agcataaagc
    36181 cagcaatacc cagcaaggtg gcgtacacca gcgagacagc aggagagatc aggcctttaa
    36241 ccagcacccg attcttcgtc ctttccatct ttctgtcgat atccctgtcg atgtagttgt
    36301 taaacacaca acccgacgcc acaaccagtg acaccccaac cagcgtgtag ataaacaggg
    36361 gataatcaat gctgcccttt gaggccagca ggaatccccc aatcaccgag atcaggttgc
    36421 caaagatgat gcctggtttc gttacttgca ggtattgctt aaacatcata accgccgctc
    36481 ttagtgcatc atcatgttgt agttgaggtt ccacataatc cagatggagc ctacaaccag
    36541 gatagtgatg attagcacgg tgaagacaaa cgccgtcatg ttccagcctt catctgattt
    36601 ggtattcatg tgcaggaagc acaccagatg caccagaacc tgtaccactg ccattgccag
    36661 gattgttccc agaattacgg ccggagaggc agcccctgtc atcaccatcc agaacggaat
    36721 caccgtcaga atgatcgaca ggataaagcc tgtcatgtag gtttttacgc tgccatggga
    36781 cgcgccgctg tgatcggtag aatgactcat tacatcgccc ccatcagata aacaacagtg
    36841 aacacacaga tccaaaccac atccaggaag tgccagaaca ggctcaggca catgatgcgg
    36901 gtacggttag tgctggtcag gccgcgacga gcgatttgta ccatcagcac cgccatccag
    36961 ataagaccgg aagtgacgtg cagaccgtgc gtgccgacca gcgcaaagaa cgctgacagg
    37021 aagccgctgc gatccggacc catgccgtta acaatcaggt gatggaattc gtagatttcc
    37081 atcccgataa atccggcacc aaacaaacag gtcaacgcca gccaggagat aacctggctt
    37141 ttgttgtttt tgtacatggc gatagccgcc atgccgtagg tgatggagct gaacaacagc
    37201 aagaaagttt caaccagaac gaacggcagt tcgaaaatgt ccttacctgt cgggccgcct
    37261 gcggtgccgt tcaccagaac ggcataggta gcaaacaaga ttgagaacag aatgcagtcg
    37321 ctcatcaggt agatccaaaa tccgaagatt ttggttccgc ctgcatcgtg gtgcccgtgt
    37381 tcgtgcgcgt gggcagtcgc gtgcgtcaaa gtatcagttg ccatttttca gccctgcctt
    37441 agtaatctca tcgaaatgct ggttttccag tttttcgatt tctgccaccg gcacgtagta
    37501 atccacgtcc tcgtcgaagc ttttcacgat ccaggtgatg atcatgcctg cgaagccaac
    37561 aatcgccagc caccagatat gccagatcat ggcgaaaccg aagatggtgg agaaagctgc
    37621 aatgacgata cctgcaccgc tgtttttcgg catatgaatt tcttcatagt ggtcaggctt
    37681 tttatacgct tcgcctttct ctttcatttc ccagaatgca tcacgctcgt gaacgtgcgg
    37741 cacaacggca aagttataga acggaggcgg ggaagagttt gcccactcca gcgtacggcc
    37801 accccacggg tcgccagtca ggtcacggtt ctggtcgcgg tcgcgaatag aaacgtacat
    37861 ctgaataacg aggcagagaa tacccagcgc aatcagtact gcaccgctag ctgcaatcat
    37921 cagcatggtg tggaactgcg ggtcaatctg ctggctcaaa cgacgggtca tgcccatgaa
    37981 gcccagcgca tacagcggca taaaggcaac gaagaagccg atgatccaga accagaacgc
    38041 gcgtttaccc caggtttcat tcagtttgaa accgaacgct ttaggccacc agtaggtcat
    38101 ccctgcgaag cagccgaaga ccacgccgcc gatgataacg ttatggaagt gcgcaatcag
    38161 gaacaggctg ttatgcagaa cgaagtccgc gcccggtact gccagcagca cgccagtcat
    38221 cccgcccacc gagaaagtga cgataaaacc gatggtccac agcatcgcag aatggaacac
    38281 gatacggccc tgatacatgg tgaacagcca gttgaagatc ttcaccccgg tcgggatggc
    38341 gataatcatt gtggtgatac caaagaaggc gtttacgttc gcgcccgcac ccatcgtaaa
    38401 gaagtggtgc agccaaacga tgaacgacag cacggtgata cagacggttg cccataccag
    38461 cgaggtataa ccaaacagac gtttacgcga gaaggttgcc gcaatttcgg agaacacacc
    38521 gaaaacaggc aggatcagga tgtaaacttc cgggtggccc caggcccaaa tcaggttgat
    38581 gtacatcatc atgttgccac ccatatcgtt ggtaaagaaa tgggtgccca gatagcgatc
    38641 cagggtcaac aacgcgacgg taaccgtcag aattgggaag gaagcaataa tcagtacgtt
    38701 cgcgcacagt gatgcccagg taaatactgg catcttgaac atggtcatgc ccggtgcgcg
    38761 catcttcaga atggtaacga agaagttgat accggtaagc gtcgtaccta taccggatag
    38821 ctggagactc catatccagt aatcaacacc gactcccgga ctgtactcta ttcccgatag
    38881 cggtggatag gccagccagc cggtctgcgc aaattcgccc acgccgagag aaacgttaac
    38941 cagaatcaca ccaacaacag taaaccagaa gcttaagttg ttgaggaacg ggaacgcaac
    39001 gtcacgcgcg ccgatctgca gcggaaccac caggttcatc agaccgataa cgaaaggcat
    39061 cgccacgaag aagatcataa tcacgccgtg cgcggtgaag atctgatcgt agtggtgagg
    39121 tggcaggaag cccgcttcgc ccgccgaggc aagagcctgc tggctacgca tcataatggc
    39181 gtcagcaaaa ccacgcagca acatcacaat cgccacgatg atatacatga taccgaggcg
    39241 tttatggtcg acggaggtca gccactcttt ccacaggtag gtccacttac cgaagtaagt
    39301 gatcaggcca acgagcgcca gacctcccaa aataatgcca gcgatcgtaa ccatgacgat
    39361 aggttcatgg aacgggactg catcaagtga taattttccg aacatcttta ttcttcctca
    39421 acccctttaa tgggcggatt ccgcgtggct catgtccatg ccttccatac cttcgtgtgc
    39481 gctgtgctca ccttctggct gggtcatgtc catgctctta ccgtgagcca taaacttgtt
    39541 aatcacatca gcaaacaagt ctggtttcac gttggagaaa tattccacct ggttgtattc
    39601 gctaggcgcg gccagttttt cgaacgcagc catgtcagac atggtgttcg gcgactgctt
    39661 cgcttttgcg acccactggt cgaattcggc gcgatccggt gttgcaatag ctttgaactt
    39721 catgcctgag aagcccgggc cgctgtagct ggcggagata ccgtcataag taccgggttc
    39781 gttggcgatc agatgcaggc gagtctgcat accagccatg gcataaatct ggctacccag
    39841 acgcggaatg aagaaggagt tcatcacgga gttggaggtc actttgaagt acaccggagt
    39901 gttcgccggg aaagcgattt cattcacggt agcaatgccc tgttccgggt agatgaagaa
    39961 ccatttccag tccatggaaa ccacttcgat ggtaatgggc ttctcgtcgt gtgccagcgg
    40021 cttgctaggc tcaagagcgt gagtggtttt ccaggtcagt accgcaagga agatgatgat
    40081 taagataggt accgtccaga ccacagcttc cactttattg gagtgtgacc agttcgggct
    40141 gtacttagca tctttattgc tcgcacgata cttccaggcg aaaccaacag ccatcaagat
    40201 tgcgggaata acgacaatca acatcaggcc aaatgccgtc agtatcagtg aacgttgctc
    40261 cagaccaatc tgtcctttgg gatctaacag cgcagaatta cagccactga gcaatacagt
    40321 gcctgcaaat aatgacaacc atcccaaact tttattgtat ttcctgagtc tcatttaacg
    40381 acctcaattc cacgggatct ggtggcgttt aaagtgtgtg ggcattttat gggaaggtta
    40441 cattactgta aacatgatta aattcctgtt acttggattt gattgtcagg tcacatatgt
    40501 tgcaaaacat atcaagttga ttttaagaag ctgttgcaaa agggcgttat aacgaaaggg
    40561 gagtgcagaa ggagtttgga tgggcaattg gtataaccaa tctggaataa aataattgtt
    40621 taacaaataa ttatcattat ggtgacaatt gggtgggggg aaaataaaga ctcgttagct
    40681 gaatcgtgta agaaaaaata gaattcataa attgcgtcaa taatttccaa acgctaagcc
    40741 gcacaaaaga acaaatatta ttaagcgata ctgatgttta tttagccgta ataattacgg
    40801 cgagtgatta ctacagctaa ataatattta cagattacgt cagatgcgtt tttcgtagag
    40861 ccagataatc cagcaaacca ccaagcacga cgccagaaag ggcgactaaa accccgactt
    40921 ccagcagagc aggcaggaaa gagaagtgcg tcaaatccag cgcgtccatc gtcaacagca
    40981 gtaaccacac ggccaacagg ctgacgcccg ccgccagtgt ccacatggca aaggcataac
    41041 ctgccggata ttcggtacgg gagataaagt tgtcatttac tcgtgtatat tcaagcgtct
    41101 ggcggcaaac cagcagcaaa ataagccccg gtacggcagc ggcgacggag aatagataga
    41161 atgtcgacca gccgtgtgct tcaacaaacc aacccgccac ggggccgaca taaactcgtc
    41221 ctacagcgga aagcgctgag agcagggcaa attgagtagc ggaaaatgac ttattacaca
    41281 gcgtcattaa cagcgcgaca aaggctgatg tgcccatccc gccacagagg ttttcgaaaa
    41341 agacggctgc gcccatgctg tagagatgct tatcagtaat cgacagcagc cagtaaccag
    41401 cgttagacgc accttgtaaa atgccgaaaa tcagcagcgc ccggaacagc gacaggcgct
    41461 gcatcaaaat cccaccgtac aatgcgccca caatggtcgc cagtaagcca agcgttttgt
    41521 taaccacgcc tacttcaccc gcatcaaacc cgacgccgcg aatcaaaaac gtggttgtca
    41581 ggctcatggc gaatgcgtcg cccagcttat acagcacgat aagaagcaaa ataagccagg
    41641 cattattgcg accaaagaaa tctcgcagag gtgcaacaac cgcttgttcc agcgtttttg
    41701 gcacaggaat ggtgtcggtc ggttctggtg caagcaacgt cgcgataata caagggatca
    41761 acagcgccgc catcaaccag tacatgccct gccagcccag ccatttatct gccagccaca
    41821 gggctaagcc gccggaaacc agcatcccta aacggtaacc cagcacgctg attgccgcgc
    41881 ccgcaccacg ttcttctgcc gggagcacat cggttttcca cgcatcgaag acaatatcct
    41941 gggaggccga gcaaaaagcg atcaccaccg ccagtgccgc catccagcgg agttgggtgc
    42001 caggttcgag aaaacccatc gccgcaatgg cgactaataa caggatttgc gtggcgagca
    42061 gccaaccgcg ccgccgccca aaaaatgggg gcgtgtagcg gtccatcagc ggtgaccaga
    42121 ggaatttaaa aacgtaagcc tggcctacca gagagaagaa accaatggtt ttgagatcaa
    42181 tattctcgac cgtcatccaa gcctgtaagg taccggatgt cagggcgagc ggtagcccgg
    42241 aagcaaaacc caggatcagc aatatggctg aacgcggctg ttgaaaaata cgtaaatatt
    42301 gactggacat gggcgtctac aggcccggtt tgcaccgggc cagagggcag aaattaacgc
    42361 gcgttctgct tgatgaattc gtgaatgctg gtgtcctgag acatatcagc gatggtatcg
    42421 gtcagcacac tgttaaccgc atcggcgata tttttgttgg aggcctggaa cgcaccttca
    42481 acgttgtagc tggcacgata gtttttggtt attttgttgc cattctgcgc ggtagcgatg
    42541 atggcgatat ccgctttggt agcgatgttg tagcgcacgt tgccctggga cacgtcagca
    42601 tacagttggc taacgatgat ttgcagatta accgggccat tcggaccaac catgtaacca
    42661 cgcgcggtca tctgtttttc cagcacttct tgcagcagga aacgcagatc gcgggaggcg
    42721 gccagggtaa cgatttgatt atcgcgggtg acttttgcca gcgcctgatc ggtacgctga
    42781 tcggcaccat taatgcttac ggtgacgccc atcaggcttg gatcctgctg tggcagtgta
    42841 atcgtcgggg aaacttcaat agttgttggc ggttttgcgc atcctgcaag cataaacaga
    42901 gcaactaacg ggaagaggat ttttttgaac atgttcgggc tctcagagac tcttaagcgt
    42961 gtttggtaaa aattcccgcc atcataacat tgccaacggc gaggggaagt gggtaaggca
    43021 tgtaaattca tcatgttgac gaaataatca cccctggtaa aagaaacgct gatgcgaggc
    43081 ctgtgtttca atctttaaat cagtaaactt catacgcttg acggaaaaac caggacgaaa
    43141 cctaaatatt tgttgttaag ctgcaatgga aacggtaaaa gcggctagta tttaaaggga
    43201 tggatgacat ctcagcgttg tcggaggaga tatttcatga tgatacgtga gcggatagaa
    43261 gaaaaattaa gggcggcgtt ccaacccgta ttcctcgaag tagtggatga aagctatcgt
    43321 cacaatgtcc cagccggctc tgaaagccat tttaaagttg tgctggtcag cgatcgtttt
    43381 acgggtgaac gttttctgaa tcgtcatcga atgatttaca gtactttagc ggaggaactc
    43441 tctactaccg ttcatgcgct ggctctgcat acttacacta ttaaggagtg ggaagggttg
    43501 caggacaccg tctttgcctc tcctccctgt cgtggagcag gaagcatcgc gtaaaaacgc
    43561 atttgcaact gtcggcgctt tttcagtatg ttgctaaaga ttttatgaaa aacggcctgc
    43621 gggccgtttt gttttgtctg gattttgcgc tttttgccca gcattcagac gaaaattgcc
    43681 cgggaattgt gaaaaaatac gcgacagcgc gcaataaccg ttctcgactc ataaaagtga
    43741 tgccgctata atgccgcgtc ctatttgaat gctttcggga tgattctggt aacagggaat
    43801 gtgattgatt ataagaacat cccggttccg cgaagccaac aacctgtgct tgcggggtaa
    43861 gagttgaccg agcactgtga ttttttgagg taacaagatg caagtttcag ttgaaaccac
    43921 tcaaggcctt ggccgccgtg taacgattac tatcgctgct gacagcatcg agaccgctgt
    43981 taaaagcgag ctggtcaacg ttgcgaaaaa agtacgtatt gacggcttcc gcaaaggcaa
    44041 agtgccaatg aatatcgttg ctcagcgtta tggcgcgtct gtacgccagg acgttctggg
    44101 tgacctgatg agccgtaact tcattgacgc catcattaaa gaaaaaatca atccggctgg
    44161 cgcaccgact tatgttccgg gcgaatacaa gctgggtgaa gacttcactt actctgtaga
    44221 gtttgaagtt tatccggaag ttgaactgca gggtctggaa gcgatcgaag ttgaaaaacc
    44281 gatcgttgaa gtgaccgacg ctgacgttga cggcatgctg gatactctgc gtaaacagca
    44341 ggcgacctgg aaagaaaaag acagcgctgt tgaagcagaa gaccgcgtaa ccatcgactt
    44401 caccggttct gtagacagcg aagagttcga aggcggtaaa gcgtctgatt tcgtactggc
    44461 gatgggccag ggtcgtatga tcccgggctt tgaagacggt atcaaaggcc acaaagctgg
    44521 cgaagagttc accatcgacg tgaccttccc ggaagaatac cacgcagaaa acctgaaagg
    44581 taaagcagcg aaattcgcta tcaacctgaa gaaagttgaa gagcgtgaac tgccggaact
    44641 gactgcagaa ttcatcaaac gtttcggcgt tgaagatggt tccgtagaag gtctgcgcgc
    44701 tgaagtgcgt aaaaacatgg agcgcgagct gaagagcgcc atccgtaacc gcgttaagtc
    44761 tcaggcgatc gaaggtctgg taaaagctaa cgacatcgac gtaccggctg cgctgatcga
    44821 cagcgaaatc gacgttctgc gtcgccaggc tgcacagcgt ttcggtggca acgaaaaaca
    44881 agctctggaa ctgccgcgcg aactgttcga agaacaggct aaacgccgcg tagttgttgg
    44941 cctgctgctg ggcgaagtta tccgcaccaa cgagctgaaa gctgacgaag agcgcgtgaa
    45001 aggcctgatc gaagagatgg cttctgcgta cgaagatccg aaagaagtta tcgagttcta
    45061 cagcaaaaac aaagaactga tggacaacat gcgcaatgtt gctctggaag aacaggctgt
    45121 tgaagctgta ctggcgaaag cgaaagtgac tgaaaaagaa accactttca acgagctgat
    45181 gaaccagcag gcgtaattta cgcagcataa cgcgctaaat tcgcacaaag gcccgtcacc
    45241 gccaggtggt gggctttttt tgtcatgaat tttgcatgga accgtgcgaa aagcctcttt
    45301 cggtgttagc gtaacaacaa aagattgtta tgcttgaaat atggtgatgc cgtacccata
    45361 acacagggac tagctgataa tccgtccata aggttacaat cggtacagca ggttttttca
    45421 attttatcca ggagacggaa atgtcataca gcggcgaacg agataacttt gcaccccata
    45481 tggcgctggt gccgatggtc attgaacaga cctcacgcgg tgagcgctct tttgatatct
    45541 attctcgtct acttaaggaa cgcgtcattt ttctgactgg ccaggttgaa gaccacatgg
    45601 ctaacctgat tgtggcgcag atgctgttcc tggaagcgga aaacccagaa aaagatatct
    45661 atctgtacat taactcccca ggcggggtga tcactgccgg gatgtctatc tatgacacca
    45721 tgcagtttat caagcctgat gtcagcacca tctgtatggg ccaggcggcc tcgatgggcg
    45781 ctttcttgct gaccgcaggg gcaaaaggta aacgtttttg cctgccgaat tcgcgcgtga
    45841 tgattcacca accgttgggc ggctaccagg gccaggcgac cgatatcgaa attcatgccc
    45901 gtgaaattct gaaagttaaa gggcgcatga atgaacttat ggcgcttcat acgggtcaat
    45961 cattagaaca gattgaacgt gataccgagc gcgatcgctt cctttccgcc cctgaagcgg
    46021 tggaatacgg tctggtcgat tcgattctga cccatcgtaa ttgatgccag aggcgcaact
    46081 gtgccgctat acttatccag ggcggcacaa cgctgtaagc ggcttgcgcc tgagaatggc
    46141 atttgcgtcg tcgtgtgcgg cacaaagaac aaagaagagg ttttgaccca tgacagataa
    46201 acgcaaagat ggctcaggca aattgctgta ttgctctttt tgcggcaaaa gccagcatga
    46261 agtgcgcaag ctgattgccg gtccatccgt gtatatctgc gacgaatgtg ttgatttatg
    46321 taacgacatc attcgcgaag agattaaaga agttgcaccg catcgtgaac gcagtgcgct
    46381 accgacgccg catgaaattc gcaaccacct ggacgattac gttatcggcc aggaacaggc
    46441 gaaaaaagtg ctggcggtcg cggtatacaa ccattacaaa cgtctgcgca acggcgatac
    46501 cagcaatggt gtcgagttgg gcaaaagtaa cattctgctg atcggtccga ccggttccgg
    46561 taaaacgctg ctggctgaaa cgctggcgcg cctgctggat gttccgttca ccatggccga
    46621 cgcgactaca ctgaccgaag ccggttatgt gggtgaagac gttgaaaaca tcattcagaa
    46681 gctgttgcag aaatgcgact acgatgtcca gaaagcacag cgtggtattg tctacatcga
    46741 tgaaatcgac aagatttctc gtaagtcgga caacccgtct attacccgtg acgtttccgg
    46801 tgaaggcgta cagcaggcac tgttgaaact gatcgaaggt actgttgctg ccgttccacc
    46861 gcagggcggg cgtaagcatc cgcagcagga atttttgcag gttgatacct ccaagatcct
    46921 gtttatttgt ggcggtgcgt ttgccggtct ggataaagtg atttcccacc gtgtagaaac
    46981 cggctccggc attggttttg gcgcgacggt aaaagcgaag tccgacaaag caagcgaagg
    47041 cgagctgctg gcgcaggttg aaccggaaga tctgatcaag tttggtctta tccctgagtt
    47101 tattggtcgt ctgccggttg tcgcaacgtt gaatgaactg agcgaagaag ctctgattca
    47161 gatcctcaaa gagccgaaaa acgccctgac caagcagtat caggcgctgt ttaatctgga
    47221 aggcgtggat ctggaattcc gtgacgaggc gctggatgct atcgctaaga aagcgatggc
    47281 gcgtaaaacc ggtgcccgtg gcctgcgttc catcgtagaa gccgcactgc tcgataccat
    47341 gtacgatctg ccgtccatgg aagatgtcga aaaagtggtt atcgacgagt cggtaattga
    47401 tggtcaaagc aaaccgttgc tgatttatgg taagccggaa gcgcaacagg catctggtga
    47461 ataattaacc attcccatac aattagttaa ccaaaaaggg gggattttat ctccccttta
    47521 atttttcctc tattctcggc gttgaatgtg ggggaaacat ccccatatac tgacgtacat
    47581 gttaatagat ggcgtgaagc acagtcgtgt catctgatta cctggcggaa attaaactaa
    47641 gagagagctc tatgaatcct gagcgttctg aacgcattga aatccccgta ttgccgctgc
    47701 gcgatgtggt ggtttatccg cacatggtca ttcccttatt tgtcgggcgg gaaaaatcta
    47761 tccgttgtct ggaagcggcg atggaccatg ataaaaaaat tatgctggtc gcgcagaaag
    47821 aagcttcaac ggatgagccg ggtgtaaacg atcttttcac cgtcgggacc gtggcctcta
    47881 tattgcagat gctgaaactg cctgacggca ccgtcaaagt gctggtcgag gggttacagc
    47941 gcgcgcgtat ttctgcgctc tctgacaatg gcgaacactt ttctgcgaag gcggagtatc
    48001 tggagtcgcc gaccattgat gagcgggaac aggaagtgct ggtgcgtact gcaatcagcc
    48061 agttcgaagg ctacatcaag ctgaacaaaa aaatcccacc agaagtgctg acgtcgctga
    48121 atagcatcga cgatccggcg cgtctggcgg ataccattgc tgcacatatg ccactgaaac
    48181 tggctgacaa acagtccgtt ctggagatgt ccgacgttaa cgaacgtctg gaatatctga
    48241 tggcaatgat ggaatcggaa atcgatctgc tgcaggttga gaaacgcatt cgcaaccgcg
    48301 ttaaaaagca gatggagaaa tcccagcgtg agtactatct gaacgagcaa atgaaagcta
    48361 ttcagaaaga actcggtgaa atggacgacg cgccggacga aaacgaagcc ctgaagcgca
    48421 aaatcgacgc ggcgaagatg ccgaaagagg caaaagagaa agcggaagca gagttgcaga
    48481 agctgaaaat gatgtctccg atgtcggcag aagcgaccgt agtgcgtggt tatatcgact
    48541 ggatggtaca ggtaccgtgg aatgcgcgca gcaaggtcaa aaaagacctg cgtcaggcgc
    48601 aggaaatcct tgataccgac cattatggtc tggagcgcgt gaaagatcgc atccttgagt
    48661 accttgcggt tcaaagccgt gtcaacaaaa tcaagggacc gatcctctgc ctggtagggc
    48721 cgccgggggt aggtaaaacc tctcttggtc agtccattgc caaagccacc gggcgtaaat
    48781 atgtccgtat ggcgctgggc ggcgtgcgtg atgaagcgga aatccgtggt caccgccgta
    48841 cttacatcgg ttctatgccg ggtaaactga tccagaaaat ggcgaaagtg ggcgtgaaaa
    48901 acccgctgtt cctgctcgat gagatcgaca aaatgtcttc tgacatgcga ggcgatccgg
    48961 cctctgcact gcttgaagtg ctggatccag agcagaacgt agcgttcagc gaccactacc
    49021 tggaagtgga ttacgatctc agcgacgtga tgtttgtcgc gacgtcgaac tccatgaaca
    49081 ttccggcacc gctgctggat cgtatggaag tgattcgcct ctccggttat accgaagatg
    49141 aaaaactgaa catcgccaaa cgtcacctgc tgccgaagca gattgaacgt aatgcactga
    49201 aaaaaggtga gctgaccgtc gacgatagcg ccattatcgg cattattcgt tactacaccc
    49261 gtgaggcggg cgtgcgtggt ctggagcgtg aaatctccaa actgtgccgc aaagcggtta
    49321 agcagttact gctcgataag tcattaaaac atatcgaaat taacggcgat aacctgcatg
    49381 actacctcgg tgttcagcgt ttcgactatg gtcgcgctga taacgaaaac tgtgtcggtc
    49441 aggtaaccgg tctggcgtgg acggaagtgg gcggtgactt gctgaccatt gaaaccgcat
    49501 gtgttccggg taaaggcaaa ctgacctata ccggttcgct cggcgaagtg atgcaggagt
    49561 ctattcaggc ggcgttaacg gtggttcgtg cgcgtgcgga aaaactgggg atcaaccctg
    49621 atttttacga aaaacgtgac atccacgtcc acgtaccgga aggtgcgacg ccgaaagatg
    49681 gtccgagtgc cggtattgct atgtgcaccg cgctggtttc ttgcctgacc ggtaacccgg
    49741 ttcgtgccga tgtggcaatg accggtgaga tcactctgcg tggtcaggta ctgccgatcg
    49801 gtggtttgaa agaaaaactc ctggcagcgc atcgcggcgg gattaaaaca gtgctaattc
    49861 cgttcgaaaa taaacgcgat ctggaagaga ttcctgacaa cgtaattgcc gatctggaca
    49921 ttcatcctgt gaagcgcatt gaggaagttc tgactctggc gctgcaaaat gaaccgtctg
    49981 gcatgcaggt tgtgactgca aaatagtgac ctcgcgcaaa atgcactaat aaaaacaggg
    50041 ctggcaggct aattcgggct tgccagcctt tttttgtctc gctaagttag atggcggatc
    50101 gggcttgccc ttattaaggg gtgttgtaag gggatggctg gcctgatata actgctgcgc
    50161 gttcgtacct tgaaggattc aagtgcgata taaattataa agaggaagag aagagtgaat
    50221 aaatctcaat tgatcgacaa gattgctgca ggggctgata tctctaaagc tgcggctggc
    50281 cgtgcgttag atgctattat tgcttccgta actgaatctc tgaaagaagg ggatgatgta
    50341 gcactggtag gttttggtac ttttgccgtt aaagagcgtg ctgcccgtac tggccgcaac
    50401 ccgcagaccg gtaaagagat caccatcgct gctgctaaag taccgagctt ccgtgcaggt
    50461 aaagcactga aagacgcggt aaactaagcg ttgtccccgg tggggatgtg acgaagttca
    50521 agggcgcatc tactgatgtg ccttttttat ttgtattcgg tgactttctg cgtcttgtgg
    50581 gctgacaatt gcccccgttt cttgtcacaa taggcctttg cgcgcatcga tacgttgcgt
    50641 gaggtacaca gtcatctaca gcggagtgtt gttacaccat gatggacagc ttacgcacgg
    50701 ctgcaaacag tctcgtgctc aagattattt tcggtatcat tatcgtgtcg ttcatattga
    50761 ccggcgtgag tggttacctg attggcggag gcaataacta cgccgcaaaa gtgaatgacc
    50821 aggaaatcag ccgtgggcag ttcgagaacg cctttaacag cgagcgtaat cgcatgcagc
    50881 aacagctggg cgatcaatac tccgagctgg cagcgaatga aggctatatg aaaaccctgc
    50941 gtcaacaggt gctgaatcgt ctgatcgacg aggcgctgtt ggatcagtac gctcgtgagc
    51001 tgaaactggg tatcagtgat gagcaggtta aacaggcgat tttcgcgacc ccagccttcc
    51061 aggttgacgg caaatttgat aacagccgct ataacggtat cctcaaccag atggggatga
    51121 ccgccgatca gtacgcccag gcgctgcgta accagctcac tacccaacag ctgattaacg
    51181 gtgttgccgg taccgatttt atgctgaaag gtgaaaccga cgagctggcg gcactggtcg
    51241 cgcaacaacg cgtggtgcgt gaggcgacta tcgatgttaa cgcgctggcg gcgaagcagc
    51301 ctgtgaccga acaggagatt gccagctact acgaacaaaa caaaaacaat ttcatgacgc
    51361 cggaacaatt ccgcgtgagt tacatcaagc tggatgccgc aacgatgcag caaccggtta
    51421 gcgatgcgga tatccagagc tactacgatc agcatcagga tcaattcacc cagccgcagc
    51481 gtacccgcta cagcatcatc cagaccaaaa ctgaagatga agcgaaagcg gtacttgatg
    51541 agctgaataa aggcggtgat tttgctgcat tagccaaaga aaaatctgcc gatattatct
    51601 ctgcccgtaa cggcggcgat atgggttggt tagaagatgc cactatcccc gacgaactga
    51661 aaaatgctgg tctgaaagaa aaaggccaac tgtctggtgt catcaaatct tcggtcggtt
    51721 tcctgattgt acgtctggac gacattcagc cagcgaaagt gaaatcgtta gacgaagtac
    51781 gtgacgacat tgcggcgaaa gtgaaacacg aaaaagccct cgatgcgtac tatgcgctgc
    51841 agcagaaagt gagcgatgcg gcaagcaacg acaccgagtc tctggccggt gcagagcaag
    51901 ctgccggcgt taaagccact cagacgggtt ggttcagcaa agataacctg ccggaagagt
    51961 tgaacttcaa gccggttgcc gacgctatct ttaacggcgg tctggtaggt gaaaacggcg
    52021 cgccgggcat caactctgac atcatcaccg tagacggcga ccgtgcattc gtgctgcgca
    52081 tcagcgagca caaaccggaa gcggtgaaac cgttggcaga tgttcaggaa caagttaagg
    52141 cactggttca gcacaacaaa gctgaacaac aggcgaaagt ggatgctgag aaactgctgg
    52201 ttgatttgaa agccggcaaa ggtgcggaag ctatgcaggc tgccggtctg aaatttggcg
    52261 agccgaaaac cttaagccgt tccggtcgtg acccgattag ccaggcggcg tttgcactgc
    52321 cactgccagc gaaagacaaa ccgagctacg gtatggcgac cgatatgcaa ggtaatgtgg
    52381 ttctgctggc gctggatgaa gtgaaacaag gttcaatgcc ggaagatcag aaaaaagcga
    52441 tggtgcaggg tatcacccag aacaacgcac aaatcgtctt tgaagctctg atgagtaacc
    52501 tgcgtaaaga ggcgaaaatc aaaattggcg atgcgctgga acagcaataa tcctgaagcc
    52561 gcctcgcaaa aaaatgcgtt gcagctgtaa caagaaaagg tcgctttcgc ggccttttcc
    52621 atttctgaac attgccattt gtttactgtt ttcactgccg ttaaggtgat tccactgtta
    52681 acaaacaagg agaaaacagt atgaaacacg gaattaaagc actgctcatt accctgtccc
    52741 tggcctgtgc cggaatgtct catagcgcgc tggcggcagc ttctgtggcg aaaccgacgg
    52801 cggtagaaac caaagcggaa gctcctgcag cacaaagtaa agcagcagta ccggcgaaag
    52861 ccagtgacga agaaggcacc cgggtcagca ttaataatgc cagcgcggaa gagctagccc
    52921 gcgcgatgaa tggcgttggc ctgaagaaag cgcaggcgat tgtcagttat cgcgaagagt
    52981 acggtccgtt taaaacagtg gaggatctaa agcaggtgcc ggggatgggc aattcgctgg
    53041 tggaacgtaa tctggcggta ttaaccctgt aattaatttg catagtggca attttttgcc
    53101 agactgaaga ggtcatacca gttatgacct ctgtacttat aacaacaacg taaggttatt
    53161 gcgctatgca aacacaaata aaagttcgtg gatatcatct cgacgtttac cagcacgtca
    53221 acaacgcccg ctaccttgaa tttctcgaag aagcccgctg ggatgggttg gaaaatagcg
    53281 acagttttca gtggatgacg gcccataaca tcgccttcgt cgtggtcaat atcaatatta
    53341 actatcgtcg cccagcggta ttaagtgacc tgttaactat taccagtcag ttgcagcaat
    53401 taaacggtaa aagcggcatc ttaagccagg tcattacact ggagccggaa gggcaggtgg
    53461 tagcggatgc gcttattacg tttgtttgta ttgatcttaa aacgcagaaa gcattagctc
    53521 tggaagggga attgcgcgaa aagctggagc agatggttaa gtaaacgttt tgtggtgccg
    53581 gatgctcaag ccgcatccgg cgacacccgg aataattacc tcaacccggt tttctgcttc
    53641 atcgctgcca tcaccgtcgg tttatcggcc agataatgat tcaaaccgtt ggcgcgtaaa
    53701 ttacatgccg cacaatgacc gcaaccgtcg cctttaatgc cgttatagca ggtcaacgtt
    53761 tcgttacgga ctaaatccag tttgccgtaa taatctgcca gcgcccaggt ttccgcttta
    53821 tcaatccaca tcagcggcgt ttcaaaacga atatctttct ccatgcccaa actgacggca
    53881 tggtttagtg ctttcacaaa ctcatcgcgg caatccgggt agccggagaa atccgtttca
    53941 cagacgccgg taattacggc ttctgctttt acctgatacg catatattgc cgccagcgtc
    54001 aggaacaaaa tattacgccc tgggacaaac gtattcggga tgccatcggc ttcaggttca
    54061 taatcaggca ccggaatgct gtcacgtgtc aggctgctga ccgccagctc gttgagcagc
    54121 gttacatcaa gaaccttatg cgcgcgtgct cccagtttca gcgccagttc gcgtgccacg
    54181 tcgatttctg cgcgatgtcg ctgaccgtaa tcgaacgtca cgcaatggac ttcatcatat
    54241 tgttgtaatg cctgcaccag acaggtggtg gaatcctggc ctccactgaa cacaacgaca
    54301 gcacgtttca tagataatcc tgcttgaaca ataaaagcgt tatggtaacg cctgcgatta
    54361 acccggacca gctattcatt gcgacggcgc gggaagccag gcttcggtaa attcaaacca
    54421 gccgcgcggt gtcagtcgca caccgttcac gccgggaggg gcactaatgc gatagtgata
    54481 gttgaacagc ggcgtcagcg tcgcgtctgc cattaactgg ctaaaaaccg ctttcagggc
    54541 attaaatcgg ttttcctcat caggcattac ttgcaccgcg tccagcgtcg attgcagatg
    54601 tgcatatgct ggagcgtcga aaacatgtgg ccatagtgga tcgcaacgta gccattgctc
    54661 cagagtatat tccggtgctt cgccaattaa tctgtcgccc atcatgaggt ctgcgtgtgc
    54721 cagtagggtc gtgtcgtccc agttttttgc gttatgaaaa ataattgtga gttcacagcc
    54781 ttccgctgcc agtgtcgcct gtaggcgttc tgccatggta tgaagttcta tcggtaggtg
    54841 ataaaccagc gtcaaggttt tcggtagttt gacttcatcc ggtacttgcc agtgcggaat
    54901 agtccagcct ggcagtaatg catgactggc ggtgatcagg ttttctccga cttctaacgt
    54961 ttgtaataaa ccggattgat gaataatgga gatcactttt cgcgcctgcc agagggagag
    55021 tcggggactt ttgcgcaacg ttaaatagca aaaacctaaa ctgatgccac tactgacctg
    55081 gctgacccgt tgcagctcct ccggtttgcc gatggtgatt tgcacgggat gccgacaact
    55141 ggttcccaaa tctttttcga aaagcggcgg agttatccag tactcaaccg ctttaagcag
    55201 cggatgacgc aaatggtaat aatcatggct ctccaggcgc accagctccg ctgtgaattg
    55261 tgttaagcga aaaggaccag tgccgatcag tgggaattgc ggatgcgcca gatggctgca
    55321 atagctcgcc agccggtgcg caagccagta atcggggcga tgtaaaaaga aggtcagaca
    55381 ctgcggatgg gtgacttcaa tacgcttcac gctaataaat aattgatcca gtgctggcag
    55441 ttgtaacagc atcaataatc gctggtgtaa gtgtgaggct tttactgcat cgccgttatg
    55501 ccagtgtagg gttgaacgaa gataaaagtc ccagcgtaac ccgtcagtag aggtttccca
    55561 gtgatgcgct aaatcgccaa tcgggcgctg agtattatta tcgaagcggg tcaggccgga
    55621 aaatatctgc ccggcgagat gctgctcggc acggccgggc aaaaagcctg gttgtagcgg
    55681 ttcgagcggg cgatagtagg gaatacgcaa cgtgggtgta tcgttttgcc attgtccgcc
    55741 cataaacggc tgtaacagag tgcgtagctc acctagggcc agttgcgcca gctccagcac
    55801 atcttgctgc tttccggttt ccagtgcctg ttccatcatc gcattgcgta gcgattccgg
    55861 cgtgaccaga aagcgtaatt gtccgcgctt tccgcgtcct gactgcgcct gccactccag
    55921 ccatcccgcc tcctgtgcct gacgcaacag cgtacgaaca tggcgttcgc tgcaaaaaca
    55981 gcgttcggcc agttcgctga cggtgacggt ttgcggcttt ccggctgaag gttgccacag
    56041 acgttgatac tggttaagac ggttgagcaa tcgcatataa acccggaaca atattattta
    56101 actattcact attacttccg tatatatcag gtgatactca atctccatta accgtgtcac
    56161 agagtggaga aagaaatggc tcgtctggca gcatttgata tggatggcac tttattgatg
    56221 cctgaccatc atttaggtga gaaaaccctc tctactttgg cgcgactgcg tgaacgcgac
    56281 attaccctca cttttgccac ggggcgtcat gcgctggaga tgcagcatat tctcggggcg
    56341 ctatcgctgg atgcatattt gattaccggc aacggaacgc gcgtgcattc tctggaaggt
    56401 gaacttttac atcgtgatga tttacctgcg gatgtcgcgg agctggtgct gtatcagcaa
    56461 tgggataccc gagccagcat gcatatcttc aatgacgacg gttggtttac cgggaaagag
    56521 atccctgcgt tgttgcaggc atttgtctat agcggttttc gttatcagat aatcgatgtc
    56581 aaaaaaatgc cactcggcag cgtcaccaag atctgcttct gtggcgatca cgacgatctt
    56641 acacgcttgc agatccagct atacgaagca ttaggcgagc gtgcacattt gtgtttttcc
    56701 gccacggatt gcctcgaagt gctgccggtg ggctgcaata aaggcgctgc attgacggtg
    56761 ctgacccaac atctaggttt atcgttgcgc gattgcatgg cctttggtga tgcgatgaac
    56821 gatcgcgaaa tgttaggcag cgtcggtagc gggtttatta tgggcaatgc gatgccgcaa
    56881 ctgcgcgcgg agctcccgca tttaccggtg attggacatt gccgaaatta ggctgtctct
    56941 cactatttga cgcactgact ggactatcca catctacctt attcccccga ataatgagat
    57001 cccttccagc accgggcaat tgcccggttt tttttgcgtt gaatttgtca ttttgtgccg
    57061 tggtgtttaa accgcacaga ataaattgtc gtgatttcac ctttaaaata gaattaaaag
    57121 agaaaaaaat tctctgtgga agggctatgt tagataaaat tgaccgtaag ctgctggcct
    57181 tactgcagca ggattgcacc ctctctttgc aggcactggc tgaagccgtt aatctgacaa
    57241 ccaccccttg ctggaagcgc ctgaaacggc tggaggacga cggtatcctt atcggcaaag
    57301 tcgccctgct ggatccggaa aaaataggcc tcggcctgac cgcctttgtg ctgataaaaa
    57361 cgcaacatca cagtagcgaa tggtattgcc gctttgtcac ggtggttacc gaaatgccag
    57421 aagtgctggg gttctggcgc atggctggtg aatacgatta tctgatgcgc gtccaggttg
    57481 ccgacatgaa acgctacgac gagttttata agcgtctggt aaacagcgtg ccggggctgt
    57541 cggacgtcac ttccagcttc gcgatggaac agattaaata caccacttct ttacccatcg
    57601 aataaatatc cagaatcagg tcaggacaca acgcgtgcga ttatttgctc aattaagctg
    57661 gtatttccgt cgggaatggc gtcgctatct cggggctgtc gccttgcttg tcattatcgc
    57721 gatgctgcaa ctggttccgc caaaagtggt tggtattgtt gtcgatggcg tgacagaaca
    57781 acactttact accgggcaga tcctgatgtg gatcgccacc atggtgctga ttgccgttgt
    57841 ggtttatttc ctgcgttacg tctggcgggt attgctgttt ggtgcgtctt atcaactggc
    57901 tgttgaactg cgtgaagatt attaccgtca gctaagccgg cagcatcctg agttttacct
    57961 gcgtcatcgc acgggtgacc tcatggctcg tgcgaccaat gacgtcgatc gcgtcgtgtt
    58021 tgcagccgga gaaggggtgc tgacgctggt ggattcactg gtgatgggct gcgctgtgtt
    58081 gattatgatg tctacgcaaa ttagctggca gttgacctta tttgccctgt tgccgatgcc
    58141 agtgatggcg atcatgatca agcgcaacgg cgatgccttg catgaacgct ttaagctggc
    58201 acaggcggcg ttttctagtc ttaatgaccg cacccaggaa agcctcacca gtatccgcat
    58261 gatcaaagcc tttggtctgg aagatcgcca gtcggcgtta tttgccgcgg atgccgaaga
    58321 taccggcaaa aaaaacatgc gggtggcgcg tattgatgct cgtttcgacc cgaccatcta
    58381 tatcgcgatt ggtatggcga atttgctggc gattggcggc ggtagttgga tggtggtgca
    58441 gggcagttta acgctgggcc agctcaccag ttttatgatg tatttaggtc tgatgatttg
    58501 gccaatgctg gcgctggcat ggatgtttaa cattgtggaa cgtggtagtg ctgcatacag
    58561 ccgtattcgc gcgatgctgg cggaagcgcc ggtggtgaac gatggtagcg aacccgtgcc
    58621 ggaagggcgt ggcgaactgg atgtaaatat tcaccagttc acgtatccgc agactgacca
    58681 tcctgcgctg gaaaacgtca atttcgccct gaaacccggt cagatgctgg gtatctgcgg
    58741 gccgactggt tccggcaaaa gtaccctgtt gtcgctcatt cagcgtcatt tcgacgtcag
    58801 cgagggggat attcgctttc atgatattcc tctgacgaag ttacaactcg atagctggcg
    58861 tagccgcctg gcggtagtta gccagacacc gttccttttt tccgacaccg tagcgaacaa
    58921 catcgcgttg ggttgcccga atgccaccca gcaagagatt gagcatgtcg cgcggttagc
    58981 cagcgtacat gacgatattt tgcgtctacc gcaaggttac gatacagaag tgggcgagcg
    59041 cggcgtgatg ctttccggtg ggcaaaaaca gcgtatctcc attgctcgag cgttattagt
    59101 caacgcggaa atcctcatcc ttgatgatgc gctttcggcg gtggacggac gcacagagca
    59161 ccagatcctg cataacctgc gtcagtgggg acagggaaga acggtaatca tcagtgccca
    59221 tcgcctttcg gcactgacgg aagccagtga aaatattgtg atgcagcacg gacatatcgc
    59281 ccagcgtggc aatcatgatg agctggcgca acaaagcggc tggtatcgcg atatgtatcg
    59341 ctatcaacaa ctggaggcgg cgctcgacga cgctccggaa attcgcgagg aggccgtcga
    59401 tgcgtagttt tagccaactg tggccgactc tcaagcgtct gttagcgtac ggttcgccgt
    59461 ggcgtaaacc gctggggatt gcggtcctga tgatgtgggt tgcggcagcg gcagaagtca
    59521 gtgggccgct gcttatcagc tattttatcg acaatatggt agcgaaaaat aacctgccgt
    59581 tgaaaatggt tgcagggctg gctgcggcgt atgttgggct acaactgttt gccgccgggc
    59641 tacattacgc gcagtcgctg ctgtttaatc gggcggcagt aggcgtagtg caacagttgc
    59701 gtaccgacgt gatggatgcc gcattgcgcc agccattaag cgagtttgat acccaacccg
    59761 tcgggcaggt gatttcccgc gtcactaatg acactgaagt gatccgcgat ctctacgtta
    59821 ccgtagtggc aactgtcctg cgtagcgccg cactggtggg cgcgatgctg gtggcgatgt
    59881 tcagcctcga ctggcgaatg gcgctggtgg cgataatgat tttcccggtg gtgctggtgg
    59941 taatggtgat ataccagcgt tacagcacgc cgattgtccg tcgtgtgcgc gcctatctgg
    60001 cggatatcaa cgacggcttt aacgaaatca tcaatggcat gagcgttatc cagcagttcc
    60061 gtcagcaggc gcgatttggc gaacgtatgg gggaggccag tcgttcacac tatatggcca
    60121 ggatgcaaac cctgcgcctc gacggttttc tgctgcgtcc gctgctgagt ctgttttcat
    60181 cgctcattct ttgtggcttg ttgatgctgt ttggcttctc cgctagcggc accattgaag
    60241 taggcgtgct gtatgcgttt atcagctatc ttgggcgact taacgaaccc ttaatcgaac
    60301 tgaccacgca acaggcgatg ctgcaacagg ctgttgttgc tggtgagcgc gtgtttgaac
    60361 tgatggacgg accgcgccag caatatggca atgatgatcg cccgttacag agtggcacca
    60421 tcgaagtcga taacgtgtca tttgcttatc gcgatgacaa tctggtgcta aagaacatta
    60481 atctctctgt gccttcgcgt aactttgtgg cactggtcgg gcataccggc agtggcaaaa
    60541 gcaccctcgc cagtttattg atgggctatt acccgctaac ggaaggtgag attcgccttg
    60601 atggtcgtcc attaagttcg ctaagtcaca gcgcgctgcg ccagggcgtg gcaatggtgc
    60661 agcaagatcc ggtggtgctg gcggatacct tcctcgccaa cgtgacgctg gggcgtgata
    60721 tctccgaaga acgcgtctgg caggcgctgg aaaccgtgca actggcggag ctggcgcgca
    60781 gcatgagtga tggtatttat acgccgctgg gcgagcaggg gaataatctc tccgtcgggc
    60841 aaaagcaact gctggcactg gcacgcgtgt tggtggagac gccgcaaatc ctgatccttg
    60901 atgaggcaac cgccagcatt gactccggta ctgaacaggc gattcaacat gctctggcgg
    60961 ctgtgcgtga acataccacg ctggtggtga ttgctcaccg cttatcgacc attgttgatg
    61021 ccgacaccat tctggtgctt catcgtgggc aagccgtgga gcagggcact caccagcaac
    61081 tgctggcggc ccagggacgc tactggcaga tgtatcaact gcaacttgcg ggcgaagagc
    61141 tggcagccag cgtgcgtgaa gaagaatcat tgagcgcctg aatagcgcaa tatttcatcg
    61201 ttggtgcaaa aatgtaacgc actgtgcact gtcatagtgc gttttcattt tcaatcttct
    61261 taacttcctg ctctctttct cgtttttcat ttctggcaca ccgcttgcaa taccttcttc
    61321 gtgtagcaga accattaccg aattctgact ggaggggatc tatgaagctg gtgaccgtga
    61381 taatcaaacc attcaagctg gaagacgttc gtgaagcgtt atcttccatt ggtattcagg
    61441 gcctgaccgt caccgaagtg aaaggtttcg ggcgtcagaa agggcatgcc gagctgtacc
    61501 ggggggcgga atacagcgtc aacttcctgc caaaagtaaa aattgatgtg gcaattgctg
    61561 atgaccaact cgatgaagtg atcgatatcg tcagtaaggc ggcttacacc ggaaaaattg
    61621 gcgacggcaa aatcttcgtc gctgaattgc aacgcgtcat tcgtattcgt accggcgaag
    61681 ccgacgaagc ggcgctgtaa tctctggcac acagcaacag gaacgaaaaa tgaagatagc
    61741 gacgataaaa actgggcttg cttcactggc gatgctgccg ggactggtaa tggctgcacc
    61801 tgcggtggcc gataaagccg acaatgcgtt tatgatgatt tgtactgcgc tggtgctgtt
    61861 tatgactatt ccggggattg ccctgtttta cggtgggttg attcgcggca aaaacgtgct
    61921 gtcgatgctg atgcaggtga cggtgacgtt tgcactggtc tgtattctct gggtggttta
    61981 cggttactcg ctggcgtttg gtgagggtaa caacttcttc ggcaacgtta actggttgat
    62041 gctgaaaaac atcgaactga cggcggtgat gggcagcatt tatcagtata tccacgtagc
    62101 gtttcaggga tcgtttgcct gcattaccgt aggcttgata gttggggcgc tggcggaacg
    62161 aatccgcttc tcagctgtgt tgattttcgt ggtggtatgg ctgacgctct cttacattcc
    62221 gattgcgcat atggtgtggg gcggcggttt gctggcttct cacggtgcac tggatttcgc
    62281 gggtggcaca gtggtgcaca ttaacgccgc aatcgccggt ctggtgggcg cgtatctgat
    62341 aggcaaacgc gtgggcttcg gtaaagaggc gtttaaaccg cacaacctgc cgatggtctt
    62401 caccgggact gccattctct atatcggttg gtttggcttt aacgccgggt cagcgggcac
    62461 ggcgaatgaa atcgcggcac tggcatttgt gaatactgtg gtcgcaacgg cggcggcaat
    62521 tcttggctgg atcttcggtg aatgggcgct gcgtggtaag ccttcactgc tgggggcgtg
    62581 ttctggcgcg attgccggtc tggtcggcgt gacgccagcg tgcggctaca ttggggttgg
    62641 cggcgcgttg attatcggcg tggtagctgg tctggcgggc ttgtggggcg ttaccatgct
    62701 caaacgcttg ctgcgggtgg atgatccctg cgatgtcttc ggtgtgcacg gcgtttgtgg
    62761 cattgtcggc tgtatcatga ccgggatttt tgccgccagc tcgctgggcg gcgtgggctt
    62821 cgctgaaggt gtgacgatgg gtcatcagtt gctggtacag ctggaaagca tcgccattac
    62881 gatcgtctgg tccggtgtgg tggcatttat tggctacaaa ttggcggatc tgacggttgg
    62941 tctgcgtgta ccggaagagc aggagctaga agggctggat gtcaacagtc acggcgagaa
    63001 tgcctataac gcgtaacaag cactgcaaaa aacagccgga cggttttcac ctccggctat
    63061 ttttttaatt gtgattacgc atcacccctt cctgaacggt cgaggcaacc agtacgccgt
    63121 cctgggtata aaactcaccg cgcacaaagc cacgtgcgct ggacgccgag gtgctctcca
    63181 cgctatacag cagccattca ttcaaattaa acgggcgatg gaaccacatg gaatggtcaa
    63241 tggtggcaat ctgaatcccc ggttcgagaa aaccgatgcc gtgcggctgt agagctaccg
    63301 gcaggaagtt aagatcagaa gcgtaaccga gcagatactg atgaacgcgc aggtcatccg
    63361 gcacgctacc atttgcgcgg atccacacct gacgatgtgg ttctgcgacg tgacctttca
    63421 gtgggttatg aaactccacc ggacggactt ccagcggacg atcgcagatg aatttatctt
    63481 taagcactgg cggcagcagg tgcgccagcg attgggcgat ttgcgtttcc gaaggcaggc
    63541 catcaggcgc tggcgcggac ggcattgttt tttgatgttc gaaacccgct tctggtgcct
    63601 ggaaagaggc cgtcatataa aaaatcggtt tgccgttttg aatagcagca acccggcggg
    63661 cgctgaagct gttaccatca cgcagcgttt cgacatcata aataatcggt ttcttactat
    63721 cgcctgggcg aagaaagtag ctgtgaaacg aatgtaccag ccgctcttcc gggacggtct
    63781 cttttgcagc atacaaggcc tgacccacga cctggccgcc aaacacctgg cgtaaaccta
    63841 aatcttcact ctggccgcga aagagtcctg cctcaatttt ttccagattt aacaatgcca
    63901 gtaaattttt tagcgcctga ctcatataac tctccagtaa caaagctgcc gcagcaagcc
    63961 aaagtgagtt gagtataacg caaatttgcc actggtccga tgggtgcaat aatctgaatt
    64021 acgggctaat tacaggcaga aatgcgtgat gtgtgccaca cttattgacg ttactatttt
    64081 gttaaccact cttccggcga ggaaagttag cccgctggtg cattgataat aaggagaaat
    64141 gaatgaaact cgtgcacatg gccagtggtt tagcggttgc gattgcgttg gcggcttgcg
    64201 cagataaaag cgcggatatt cagacgccag ccccggctgc aaatacgtct atttcagcaa
    64261 cacaacaacc tgctatccag caaccgaatg tctccggtac cgtctggatc cggcagaaag
    64321 tcgcactgcc gcctgatgct gtgctgaccg tgacactttc tgacgcgtcg ttagccgatg
    64381 caccgtcaaa agtgctggcg cagaaagcgg tgcgtaccga aggtaaacag tcaccattca
    64441 gctttgttct gccatttaac ccggcagatg ttcagccgaa cgcgcgtatt ctgttgagtg
    64501 cggcgattac cgtgaatgac aaactggtat ttattaccga taccgttcag ccggtgatca
    64561 acaagggcgg aactaaagcc gacctgacat tggtgccggt gcagcaaacc gccgtgccgg
    64621 ttcaggccag cggtggtgca acgactaccg taccttcgac ttcaccaact caggtgaatc
    64681 cgtcttcggc agttccagct cctacgcaat attaagcgac gttaaccctc tccgcccg
//
LOCUS       DAJTSL010000004        62760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTSL010000004 DAJTSL010000000
VERSION     DAJTSL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.004"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            414..1616
                     /gene="proV"
                     /locus_tag="OO433_000214"
     CDS             414..1616
                     /gene="proV"
                     /locus_tag="OO433_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6048834.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            1609..2673
                     /gene="proW"
                     /locus_tag="OO433_000215"
     CDS             1609..2673
                     /gene="proW"
                     /locus_tag="OO433_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6048835.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQVLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPSIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2731..3723
                     /gene="proX"
                     /locus_tag="OO433_000216"
     CDS             2731..3723
                     /gene="proX"
                     /locus_tag="OO433_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6048836.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTLLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKEPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAALK"
     gene            4015..5199
                     /locus_tag="OO433_000217"
     CDS             4015..5199
                     /locus_tag="OO433_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6048837.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRPRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            5323..6060
                     /gene="ygaZ"
                     /locus_tag="OO433_000218"
     CDS             5323..6060
                     /gene="ygaZ"
                     /locus_tag="OO433_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6048838.1"
                     /translation="MESPTPQPALGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            6050..6385
                     /gene="ygaH"
                     /locus_tag="OO433_000219"
     CDS             6050..6385
                     /gene="ygaH"
                     /locus_tag="OO433_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6048839.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6476..7006
                     /gene="emrR"
                     /locus_tag="OO433_000220"
     CDS             6476..7006
                     /gene="emrR"
                     /locus_tag="OO433_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6048840.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            7133..8305
                     /gene="emrA"
                     /locus_tag="OO433_000221"
     CDS             7133..8305
                     /gene="emrA"
                     /locus_tag="OO433_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR6048841.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTVREISLAPVNKLIDDIVKANAG"
     gene            8322..9860
                     /gene="emrB"
                     /locus_tag="OO433_000222"
     CDS             8322..9860
                     /gene="emrB"
                     /locus_tag="OO433_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6048842.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWECGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9924..10439)
                     /gene="luxS"
                     /locus_tag="OO433_000223"
     CDS             complement(9924..10439)
                     /gene="luxS"
                     /locus_tag="OO433_000223"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6048843.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10589..12145)
                     /gene="gshA"
                     /locus_tag="OO433_000224"
     CDS             complement(10589..12145)
                     /gene="gshA"
                     /locus_tag="OO433_000224"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6048844.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTQATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGTDAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGKAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(12218..12646)
                     /locus_tag="OO433_000225"
     CDS             complement(12218..12646)
                     /locus_tag="OO433_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6048845.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12643..13209)
                     /gene="yqaB"
                     /locus_tag="OO433_000226"
     CDS             complement(12643..13209)
                     /gene="yqaB"
                     /locus_tag="OO433_000226"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6048846.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLAPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13357..13433)
                     /locus_tag="OO433_000227"
     tRNA            complement(13357..13433)
                     /locus_tag="OO433_000227"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13397..13399),aa:Arg,seq:acg)
     gene            complement(13632..13708)
                     /locus_tag="OO433_000228"
     tRNA            complement(13632..13708)
                     /locus_tag="OO433_000228"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13672..13674),aa:Arg,seq:acg)
     gene            complement(13906..13982)
                     /locus_tag="OO433_000229"
     tRNA            complement(13906..13982)
                     /locus_tag="OO433_000229"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13946..13948),aa:Arg,seq:acg)
     gene            complement(13986..14078)
                     /locus_tag="OO433_000230"
     tRNA            complement(13986..14078)
                     /locus_tag="OO433_000230"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14042..14044),aa:Ser,seq:gct)
     gene            complement(14394..14579)
                     /gene="csrA"
                     /locus_tag="OO433_000231"
     CDS             complement(14394..14579)
                     /gene="csrA"
                     /locus_tag="OO433_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6048847.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14814..17444)
                     /gene="alaS"
                     /locus_tag="OO433_000232"
     CDS             complement(14814..17444)
                     /gene="alaS"
                     /locus_tag="OO433_000232"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6048848.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKDANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEVAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17572..18072)
                     /gene="recX"
                     /locus_tag="OO433_000233"
     CDS             complement(17572..18072)
                     /gene="recX"
                     /locus_tag="OO433_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6048849.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYDEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(18315..19376)
                     /gene="recA"
                     /locus_tag="OO433_000234"
     CDS             complement(18315..19376)
                     /gene="recA"
                     /locus_tag="OO433_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6048850.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19456..19953)
                     /gene="pncC"
                     /locus_tag="OO433_000235"
     CDS             complement(19456..19953)
                     /gene="pncC"
                     /locus_tag="OO433_000235"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6048851.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(20098..21183)
                     /gene="mltB"
                     /locus_tag="OO433_000236"
     CDS             complement(20098..21183)
                     /gene="mltB"
                     /locus_tag="OO433_000236"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6048852.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21439..22002
                     /gene="srlA"
                     /locus_tag="OO433_000237"
                     /gene_synonym="gutA"
     CDS             21439..22002
                     /gene="srlA"
                     /locus_tag="OO433_000237"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR6048853.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSQGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLALSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            21999..22958
                     /gene="srlE"
                     /locus_tag="OO433_000238"
                     /gene_synonym="gutE"
     CDS             21999..22958
                     /gene="srlE"
                     /locus_tag="OO433_000238"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6048854.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            22969..23340
                     /gene="srlB"
                     /locus_tag="OO433_000239"
                     /gene_synonym="gutB"
     CDS             22969..23340
                     /gene="srlB"
                     /locus_tag="OO433_000239"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6048855.1"
                     /translation="MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            23344..24123
                     /gene="srlD"
                     /locus_tag="OO433_000240"
     CDS             23344..24123
                     /gene="srlD"
                     /locus_tag="OO433_000240"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6048856.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLVAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            24228..24586
                     /gene="gutM"
                     /locus_tag="OO433_000241"
                     /pseudo
     CDS             24228..24586
                     /gene="gutM"
                     /locus_tag="OO433_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            24653..25426
                     /gene="srlR"
                     /locus_tag="OO433_000242"
                     /gene_synonym="gutR"
     CDS             24653..25426
                     /gene="srlR"
                     /locus_tag="OO433_000242"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6048857.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25419..26384
                     /gene="gutQ"
                     /locus_tag="OO433_000243"
     CDS             25419..26384
                     /gene="gutQ"
                     /locus_tag="OO433_000243"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6048858.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26381..27895)
                     /gene="norR"
                     /locus_tag="OO433_000244"
     CDS             complement(26381..27895)
                     /gene="norR"
                     /locus_tag="OO433_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6048859.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            28082..29521
                     /gene="norV"
                     /locus_tag="OO433_000245"
     CDS             28082..29521
                     /gene="norV"
                     /locus_tag="OO433_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6048860.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNKVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            29518..30651
                     /gene="norW"
                     /locus_tag="OO433_000246"
                     /pseudo
     CDS             29518..30651
                     /gene="norW"
                     /locus_tag="OO433_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
     gene            complement(30779..33031)
                     /gene="hypF"
                     /locus_tag="OO433_000247"
     CDS             complement(30779..33031)
                     /gene="hypF"
                     /locus_tag="OO433_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6048861.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPKYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAQVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(33184..33711)
                     /gene="hydN"
                     /locus_tag="OO433_000248"
     CDS             complement(33184..33711)
                     /gene="hydN"
                     /locus_tag="OO433_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6048862.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33860..34870)
                     /gene="ascG"
                     /locus_tag="OO433_000249"
     CDS             complement(33860..34870)
                     /gene="ascG"
                     /locus_tag="OO433_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6048863.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            35130..36587
                     /gene="ascF"
                     /locus_tag="OO433_000250"
     CDS             35130..36587
                     /gene="ascF"
                     /locus_tag="OO433_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR6048864.1"
                     /translation="MAKNYAALARSEIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTPRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYSIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            36596..38020
                     /gene="ascB"
                     /locus_tag="OO433_000251"
     CDS             36596..38020
                     /gene="ascB"
                     /locus_tag="OO433_000251"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR6048865.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LAAKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(38179..38649)
                     /gene="hycI"
                     /locus_tag="OO433_000252"
     CDS             complement(38179..38649)
                     /gene="hycI"
                     /locus_tag="OO433_000252"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR6048866.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(38642..39052)
                     /gene="hycH"
                     /locus_tag="OO433_000253"
     CDS             complement(38642..39052)
                     /gene="hycH"
                     /locus_tag="OO433_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR6048867.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(39049..39816)
                     /gene="hycG"
                     /locus_tag="OO433_000254"
     CDS             complement(39049..39816)
                     /gene="hycG"
                     /locus_tag="OO433_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR6048868.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEVRIR"
     gene            complement(39816..40358)
                     /gene="hycF"
                     /locus_tag="OO433_000255"
     CDS             complement(39816..40358)
                     /gene="hycF"
                     /locus_tag="OO433_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR6048869.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(40368..42077)
                     /gene="hycE"
                     /locus_tag="OO433_000256"
     CDS             complement(40368..42077)
                     /gene="hycE"
                     /locus_tag="OO433_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6048870.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKEQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(42095..43018)
                     /gene="hycD"
                     /locus_tag="OO433_000257"
     CDS             complement(42095..43018)
                     /gene="hycD"
                     /locus_tag="OO433_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6048871.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITSRITWAGFGFAFLAFVSLLAA"
     gene            complement(43021..44847)
                     /gene="hycC"
                     /locus_tag="OO433_000258"
     CDS             complement(43021..44847)
                     /gene="hycC"
                     /locus_tag="OO433_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6048872.1"
                     /translation="MSAISLINSGVVWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILILSLLGGNAPLWWGIVLLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(44844..45455)
                     /gene="hycB"
                     /locus_tag="OO433_000259"
     CDS             complement(44844..45455)
                     /gene="hycB"
                     /locus_tag="OO433_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6048873.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNVITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(45580..46041)
                     /gene="hycA"
                     /locus_tag="OO433_000260"
     CDS             complement(45580..46041)
                     /gene="hycA"
                     /locus_tag="OO433_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6048874.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            46253..46603
                     /gene="hypA"
                     /locus_tag="OO433_000261"
     CDS             46253..46603
                     /gene="hypA"
                     /locus_tag="OO433_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6048875.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEVECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            46607..47479
                     /gene="hypB"
                     /locus_tag="OO433_000262"
     CDS             46607..47479
                     /gene="hypB"
                     /locus_tag="OO433_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6048876.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            47470..47742
                     /gene="hypC"
                     /locus_tag="OO433_000263"
     CDS             47470..47742
                     /gene="hypC"
                     /locus_tag="OO433_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6048877.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVITEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            47742..48863
                     /gene="hypD"
                     /locus_tag="OO433_000264"
     CDS             47742..48863
                     /gene="hypD"
                     /locus_tag="OO433_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6048878.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            48860..49870
                     /gene="hypE"
                     /locus_tag="OO433_000265"
     CDS             48860..49870
                     /gene="hypE"
                     /locus_tag="OO433_000265"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6048879.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPCYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            49944..52022
                     /gene="flhA"
                     /locus_tag="OO433_000266"
                     /pseudo
     CDS             49944..52022
                     /gene="flhA"
                     /locus_tag="OO433_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(52059..52412)
                     /locus_tag="OO433_000267"
     CDS             complement(52059..52412)
                     /locus_tag="OO433_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6048880.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(52489..52623)
                     /locus_tag="OO433_000268"
     CDS             complement(52489..52623)
                     /locus_tag="OO433_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6048881.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            52699..55260
                     /gene="mutS"
                     /locus_tag="OO433_000269"
     CDS             52699..55260
                     /gene="mutS"
                     /locus_tag="OO433_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6048882.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     DVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            55366..56022
                     /gene="pphB"
                     /locus_tag="OO433_000270"
     CDS             55366..56022
                     /gene="pphB"
                     /locus_tag="OO433_000270"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCR6048883.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMELDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     WPVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(56073..56840)
                     /locus_tag="OO433_000271"
     CDS             complement(56073..56840)
                     /locus_tag="OO433_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6048884.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            57036..57944
                     /locus_tag="OO433_000272"
     CDS             57036..57944
                     /locus_tag="OO433_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6048885.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGDAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            57941..59203
                     /locus_tag="OO433_000273"
     CDS             57941..59203
                     /locus_tag="OO433_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6048886.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            59200..59838
                     /locus_tag="OO433_000274"
     CDS             59200..59838
                     /locus_tag="OO433_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6048887.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTVLSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            59843..60618
                     /locus_tag="OO433_000275"
                     /pseudo
     CDS             59843..60618
                     /locus_tag="OO433_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
     gene            60707..62071
                     /locus_tag="OO433_000276"
     CDS             60707..62071
                     /locus_tag="OO433_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR6048888.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVSFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVATAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMTGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(62165..>62760)
                     /locus_tag="OO433_000277"
     CDS             complement(62165..>62760)
                     /locus_tag="OO433_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR6048889.1"
                     /translation="FDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNV
                     YLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALL
                     DILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGR
                     EIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
BASE COUNT        14732 a        16977 c        16318 g        14733 t
ORIGIN      
        1 gctcctctta tgtgatgggg aaagcggttg aaacagaaga tgaagactgg aatttctgag
       61 ggtgttattt tcaaaaatat cactacccgc agcagggaaa taattcccgc caaatagctt
      121 tttatcacgc aaatattctg tagtgatcta cactgagact ctgttgcatt attcgcctga
      181 aactacaata ttcaggcgtt ttttcgctat ctatgacaaa aaatatcaac ttttctcgat
      241 ttgctctcag cccttatatc acgggaaatt ccggcgattt gctcgcatca atattcatgc
      301 cacatttgcc ctcaggggtt gcctcagatt ctcagtatgt tagggtagaa aaaagtgact
      361 atttccattg ggtaatatat cgacataaac aaataaagga atctttctat tgcatggcaa
      421 ttaaattaga aattaaaaat ctttataaaa tatttggcga gcatccacag cgagcgttca
      481 aatatatcga acaaggactt tcgaaagaac aaattctgga aaaaactggg ctatcgcttg
      541 gcgtaaaaga cgccagtctg gccattgaag aaggcgagat atttgtcatc atgggattat
      601 ccggctcggg taaatccaca atggtacgcc ttctcaatcg cctgattgaa cccacccgcg
      661 ggcaagtgct gattgatggt gtggatattg ccaaaatatc cgatgccgaa ctccgtgagg
      721 tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc cttaatgccg catatgaccg
      781 tgctggacaa tactgcgttc ggtatggaat tggccggaat taatgccgaa gaacgccggg
      841 aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa ttatgcccac agctacccgg
      901 atgaactctc tggcgggatg cgtcaacgtg tgggattagc ccgcgcgtta gcgattaatc
      961 cggatatatt attaatggac gaagccttct cggcgctcga tccattaatt cgcaccgaga
     1021 tgcaggatga gctggtaaaa ttacaggcga aacatcagcg caccattgtc tttatttccc
     1081 acgatctcga tgaagctatg cgtattggcg accgaattgc cattatgcaa aatggtgaag
     1141 tggtacaggt cggcacaccg gatgaaattc tcaataatcc ggcgaatgat tatgtccgta
     1201 ccttcttccg tggcgttgat attagtcagg tattcagtgc gaaagatatt gcccgccgga
     1261 caccgaatgg cttaattcgt aaaacccctg gcttcggccc acgttcggca ctgaaattat
     1321 tgcaggatga agatcgcgaa tatggctacg ttatcgaacg cggtaataag tttgtcggcg
     1381 cagtttccat cgattcgctt aaaaccgcgt taacgcagca gcaaggtctt gatgcggcgc
     1441 tgattgatgc gccgttagca gtcgatgcac aaacgcctct tagcgagttg ctctctcatg
     1501 tcggacaggc accctgtgcg gtacccgtgg tcgacgagga ccaacagtat gtcggcatca
     1561 tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg ggtaaataat ggctgatcaa
     1621 aataatccgt gggataccac gccagcggcg gacagtgccg tgcaatccgc agacgcctgg
     1681 ggtacaccgg cgactgcacc gactgacggc ggtggtgctg actggctgac cagtacgcct
     1741 gcgccaaacg tcgagcattt taatattctc gatccgttcc ataaaacgct gatcccgctc
     1801 gacagttggg tcactgaagg gatcgactgg gttgttaccc atttccgtcc cgtcttccag
     1861 ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc agcaagtgct gctgggtatg
     1921 cccgcaccgg tggcgattat cgttttcgct ctcatcgcct ggcagatttc cggggtcgga
     1981 atgggcgtgg cgacgctggt ttcgctgatt gccatcggcg caatcggtgc ctggtcgcag
     2041 gccatggtta ccctggcgct ggtgttaacc gccctgctgt tctgtatcgt cattggtttg
     2101 ccgctgggga tctggctggc gagaagtccg cgagcggcga aaattattcg tccactgctt
     2161 gatgccatgc agaccacgcc agcgtttgtt tatctggtgc caatcgtcat gctatttggt
     2221 atcggtaacg tgccgggcgt ggtggtgacg atcatctttg ctctgccgcc gattatccgt
     2281 ctgaccattc tggggattaa ccaggttccg gcggatctga ttgaagcctc gcgctcattc
     2341 ggtgccagcc cgcgccagat gctgttcaaa gttcagttac cgctggcgat gccgagcatt
     2401 atggcgggcg ttaaccagac gctgatgctg gccctttcta tggtggtcat tgcctcgatg
     2461 attgccgtcg gcgggctggg tcagatggta cttcgcggta tcggtcgtct ggatatgggg
     2521 cttgccaccg ttggcggcgt cgggattgtg atcctcgcca ttatcctcga tcgcctgacg
     2581 caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc gctggtacac cactggccct
     2641 gttggtctgc tgacccgccc attcattaag taactctgca cttgcccggt gacgccgggc
     2701 attatcaccc tgccaaaaaa aggaataaca atgcgacata gcgtactttt tgcgacagcg
     2761 tttgccacgc ttatctctac acaaactttt gctgccgatc tgccgggcaa aggtattact
     2821 gttaatccag ttcaaagcac catcaccgaa gaaaccttcc agacgctgct ggtcagccgt
     2881 gcgctggaga aattaggcta taccgtcaac aaacccagcg aagtagatta caacgttggc
     2941 tacaccttgc ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac gccactgcat
     3001 gacaacatgt acgaagctgc cggtggcgat aagaaatttt atcgtgaagg ggtatttgtt
     3061 aacggcgcgg cacagggtta cctgatcgat aagaaaaccg ccgaccagta caaaatcacc
     3121 aacatcgcac aactgaaaga gccgaagatc gccaaactgt tcgataccaa cggcgacggt
     3181 aaagcggatt taaccggttg taatcctggc tggggctgcg aaggtgcgat caaccaccag
     3241 cttgccgcgt atgaactgac caacaccgtg acgcataatc aggggaacta cgcagcgatg
     3301 atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg tgttttatta cacctggacg
     3361 ccgtactggg tgagtaatga gctgaagcca gggaaagatg tggtctggtt gcaggtgccg
     3421 ttctccgcac tgccgggcga taaaaatgcc gataccaaac tgccgaatgg cgcgaattac
     3481 ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag cctggaccga gaaaaatccg
     3541 gcggcagcca aactgtttgc cattatgcag ttgccagtgg ccgatattaa cgcccagaac
     3601 gccattatgc atgacggcaa agcctcagaa ggcgatattc agggccacgt tgatggctgg
     3661 atcaaagccc accagcagca gttcgatggc tgggtgaatg aggcgctggc agcgctgaag
     3721 taatttttat tcaggcggat aaggcgtttt tgccttatcc gccgctctgt acacaatgcc
     3781 tgatgcgacg ctgacgcgtc ttatcatgcc tacaaaatac actcattccg tatggcggat
     3841 aaggcgtttt cgccgcatcc gccgttctgt gcacaatgcc tgatgcgacg ctggcgcgtc
     3901 ttatcatgcc tacaaaccac atcgcacatt tcaacaagct atctttcatc ccatattcat
     3961 caacatccgc tattattgat ttccagctta atcatcacct gatgaacaaa aataatgact
     4021 aaacctaatc atgagcttag cccggcgctg atcgtgctga tgtctatcgc caccggtctg
     4081 gcggtagcca gtaactatta cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc
     4141 ctttccgcca gttcggcagg ctttattgtt accgccgcgc aattgggcta tgcagcaggt
     4201 ctgctgtttc ttgttcccct cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg
     4261 accttactgg cggcaggcgg catgttaatc actgccagca gtcagtcgct ggcgatgatg
     4321 atcctcggta cggcattaac cggtttattc tctgtcgtgg cacaaattct ggttccgctg
     4381 gcagcgacgc tggcttcacc ggacaaacgc ggcaaagtgg ttggcactat tatgagcggt
     4441 ctgctgttgg ggatcttact cgcacggacc gttgccggat tgctggcgaa tctcggcggc
     4501 tggcgcaccg tcttttgggt tgcctcggtg ttaatggcac tgatggcgct ggcattatgg
     4561 cgtggtctgc cacaaatgaa atcagaaacc cacctcaact acccacagtt gttaggttcc
     4621 gttttcagca tgtttatcag cgataagatc ctgcgccccc gcgcattgct gggctgcctg
     4681 acctttgcca acttcagcat tctctggacc tcaatggcct ttttgcttgc cgctccacct
     4741 tttaactaca gcgatggtgt cattggtctg tttggacttg cgggagctgc cggagcgttg
     4801 ggcgctcgtc cggcgggcgg ttttgccgat aagggtaaat cgcaccacac cacaactttc
     4861 ggtctgctgc tgctattact ttcatggctg gcgatctggt ttggtcacac ttccgtactg
     4921 gcgctgatta tcggcatcct ggtgctggat ctcaccgtac agggcgtgca tatcactaac
     4981 cagacggtaa tttatcgaat acaccctgat gcgcgtaatc gcctgaccgc aggttacatg
     5041 accagctact ttattggcgg tgccgccggt tcgctaattt cagcctcagc ctggcaacat
     5101 ggcggttggg ctggcgtttg tctggctggc gcgacgattg ccctggttaa cttactggtc
     5161 tggtggcgag gttttcatcg tcaggaagcc gcaaattaag caatcagcaa ccgttttcgt
     5221 ggctttacac ttataagggt gttaagaagc ccatcagtct gataaggtta agatattcat
     5281 tcagtctatt tataatatta acaatcgtta agcgtacact ctatggaaag ccctactcca
     5341 cagcctgctc ttggttcggc gaccttcatg gaaggatgca aagacagttt accgattgtt
     5401 attagttata ttccggtggc ctttgcgttc ggtctgaatg cgacccgtct gggattctct
     5461 cctctcgaaa gcgttttttt ctcctgcatc atttatgcag gcgcgagcca gttcgtcatt
     5521 accgcgatgc tggcagccgg gagtagtttg tgggttgctg cactgaccgt catggcaatg
     5581 gatgttcgcc atgtgttgta tggcccgtca ctgcgtagcc gtattattca gcgtctgcaa
     5641 aaatcgaaaa ccgccctgtg ggcatttggc ctgacggatg aggtttttgc cgccgcaacc
     5701 gcaaaactgg tacgcaataa tcgccgctgg agcgagaact ggatgatcgg cattgccttc
     5761 agttcatggt catcgtgggt atttggtacg gtaatagggg cattctccgg cagcggcttg
     5821 ctgcaaggtt atcccgccgt tgaagctgca ttaggtttta tgcttccggc actctttatg
     5881 agtttcctgc tcgcctcttt ccagcgcaaa caatctcttt gcgttaccgc agcgttagtt
     5941 ggtgcccttg caggcgtaac gctattttct attcccgtcg ccattctggc aggcattgtc
     6001 tgtggctgcc tcactgcgtt aatccaggca ttctggcaag gagcgcccga tgagctatga
     6061 ggttctgctg cttgggttac tggttggcgt ggcgaattat tgcttccgct atttgccgct
     6121 gcgcctgcgt gtgggtaatg cccgcccaac caaacgtggc gcggtaggta ttttgctcga
     6181 caccattggc atcgcctcga tatgcgctct gctggttgtc tctaccgcac cagaagtgat
     6241 gcacgataca cgccgtttcg tgcccacgct ggtcggcttc gcggtactgg gtgccagttt
     6301 ctataaaaca cgcagcatta tcatcccaac actgcttagt gcgctggcct atgggctcgc
     6361 ctggaaagtg atggcgatta tataacccac aagaatcatt tttctaaaac aatacattta
     6421 ctttatttgt tactgtcgtt actatatcgg ctgaaattaa tgaggtcata cccaaatgga
     6481 tagttcgttt acgcccattg aacaaatgct aaaatttcgc gccagccgcc acgaagattt
     6541 tccttatcag gagatccttc tgactcgtct ttgcatgcac atgcaaagca agctgctgga
     6601 gaaccgcaat aaaatgctga aggctcaggg gattaacgag acgttgttta tggcgttgat
     6661 tacgctggag tctcaggaaa accacagtat tcagccttct gaattaagtt gtgctcttgg
     6721 atcatcccgt accaacgcga cgcgtattgc cgatgaactg gaaaaacgcg gttggatcga
     6781 acgtcgtgaa agcgataacg atcgccgctg cctgcatctg caattaacgg aaaaaggtca
     6841 cgagtttttg cgcgaggttt taccaccgca gcataactgc ctgcatcaac tctggtccgc
     6901 gctcagcaca acagaaaaag atcagctcga gcaaatcacc cgcaaattgc tctcccgtct
     6961 cgaccagatg gaacaagacg gtgtggttct cgaagcgatg agctaacgcg tcatctcgct
     7021 caaaaatcca gatttataaa agaaaaaatg accggccagc atcgcaacat gctggccttt
     7081 ttggcaagca ggtcggctca gccgatgagt taagaagatc gtggagaaca atatgagcgc
     7141 aaatgcggag actcaaaccc cgcagcaacc ggtaaagaag agcggcaaac gtaagcgtct
     7201 gctcctcctt ctcaccttgc tctttataat tattgccgta gcgataggga tttattggtt
     7261 tttggtactg cgtcatttcg aagaaaccga tgacgcatac gtggcaggga atcagattca
     7321 aattatgtct caggtgtctg gcagcgtgac gaaagtctgg gccgataaca ccgattttgt
     7381 aaaagaaggc gatgttctgg tcactctcga cccgacagat gctcgccagg cgtttgaaaa
     7441 agccaaaact gcactggctt ccagcgttcg ccaaacccac cagctgatga ttaacagcaa
     7501 acagttacag gcgaatattg aggtgcaaaa aatcgccctc gcgaaagcac aaagcgacta
     7561 caaccgccgt gtgccgctgg gcaatgccaa cctgattggt cgcgaagagc tgcaacacgc
     7621 ccgcgacgcc gtcaccagtg cccaggcgca actggacgtc gctattcaac aatacaatgc
     7681 caatcaggcg atgattctgg ggactaaact ggaagatcag cctgccgtgc aacaggctgc
     7741 caccgaagta cgtaacgcct ggctggcgct ggagcgtacc cgtattgtca gtccgatgac
     7801 cggttatgtc tcccgccgcg cggtacagcc tggggcgcaa attagcccaa cgacgccgct
     7861 gatggcggtc gttccagcca ccaatatgtg ggtggatgcc aactttaaag agacgcagat
     7921 tgccaatatg cgtatcggtc agccggtcac tatcaccacg gatatttacg gcgatgatgt
     7981 gaaatacacc ggtaaagtgg ttggtctgga tatgggcaca ggtagcgcat tctcactgct
     8041 tccggcgcaa aatgcgaccg gtaactggat caaagtcgtt cagcgtctac ctgtgcgtat
     8101 cgaactggac cagaaacagc tggagcaata cccgctgcgc attggtttgt ccacgctggt
     8161 gagcgtcaat accactaacc gtgacggtca ggtactggca aataaagtac gttcaacacc
     8221 ggtcgcggta agtaccgtgc gtgaaatcag cctggcacct gtcaataaac tgatcgacga
     8281 tatcgtaaaa gctaacgctg gctaatccag aggtgcgtgt gatgcaacag caaaaaccgc
     8341 tggaaggcgc gcaactggtc attatgacga ttgcgctgtc actggcgaca ttcatgcagg
     8401 tgctggactc caccattgct aacgtggcga tccccactat cgccgggaat ctgggctcat
     8461 cgctcagcca gggaacgtgg gtaatcactt ctttcggggt ggcgaatgcc atctcgatcc
     8521 cgcttaccgg ctggctggca aagcgcgtcg gggaagtgaa actgttcctt tggtccacca
     8581 tcgcctttgc tattgcgtcg tgggagtgtg gtgtctccag cagcctgaat atgctgatct
     8641 tcttccgtgt gattcagggg attgtcgctg ggccgttgat cccactttcg caaagtcttt
     8701 tgctgaataa ttacccgcca gccaaacgct caatcgcgct ggcgttgtgg tcgatgacgg
     8761 tgattgtcgc gccaatttgc ggcccaatcc tcggcggtta tatcagcgat aattaccact
     8821 ggggctggat attcttcatc aacgtgccaa ttggcgtggc ggtggtgttg atgacactgc
     8881 aaaccctgcg cggacgcgaa acccgcaccg aacggcggcg gattgatgcc gtggggctgg
     8941 cactgctggt tattggtatc ggtagcctgc agattatgct cgaccgcggt aaagagctgg
     9001 actggttttc atcacaggaa attatcattc ttaccgtggt ggcggtggtg gctatctgct
     9061 tcctgattgt ctgggagctg accgacgata acccgatagt cgatctgtcg ttgtttaagt
     9121 cgcgcaactt caccatcggc tgcttgtgta tcagcctcgc gtatatgctc tacttcggcg
     9181 cgattgttct gctgccgcag ttgttgcagg aggtctacgg ttacacggcg acctgggcag
     9241 gtttggcctc tgcgccggta gggattattc cggtgatcct gtcgccgatt atcggccgct
     9301 tcgcgcataa actggatatg cggcggctgg taaccttcag ctttattatg tatgccgtct
     9361 gcttctactg gcgtgcctat acctttgaac caggtatgga ttttggcgcg tcggcctggc
     9421 cgcagtttat ccaggggttt gcggtggcct gcttctttat gccgctgacc accattacgc
     9481 tgtctggttt gccaccggaa cgactggcgg cggcatcgag cctctctaac tttacgcgaa
     9541 cgctggcggg gtctatcggc acgtcgataa ccacgaccat gtggaccaac cgcgagtcga
     9601 tgcaccatgc gcagttgact gagtcggtaa acccgttcaa cccgaatgcc caggcgatgt
     9661 acagtcaact ggaagggctt gggatgacgc aacagcaggc atcaggctgg attgcccagc
     9721 agatcactaa tcaggggctg attatttccg ccaatgagat cttctggatg tcagccggga
     9781 tattcctcgt cctgctgggg ctggtgtggt ttgctaaacc gccctttggc gcaggtggcg
     9841 gcggaggtgg tgcgcactaa gtacaactaa gccagttcat ttgaactggc ttttttcaat
     9901 taattgtgaa gatagtttac tgactaaatg tgcagttcct gcaacttctc tttcggcagt
     9961 gccagttctt cgttgctgtt gatgcgtacg tcacgttcca gaatgctacg cgcaatatcc
    10021 tgcgcttcct gcaacgagtg catctggtaa gtgccacact ggtagacgtt cagctccggg
    10081 atctgattct gatcctgcac tttcagcacg tcttccattg ccgctttcca ggcatcagca
    10141 acacgctgct catctggcgt accaatcaga ctcatataaa aaccggtgcg gcagcccatt
    10201 ggcgagatat cgataatctc tacaccatta ccgttaagat ggttacgcat aaaaccagca
    10261 aacaggtgct ccagagtatg gctccctctt tctggcatca cttctttgtt cggcacgcag
    10321 aagcgcagat cgaacacggt gattgcgtcg ccatgcgggg tgttcattgt tttcgccacc
    10381 cgaactgcag gcgcttccat ccgggtatga tcgactgtga agctatctaa caacggcatt
    10441 tagccacctc cggtaatttt tttaaaaatt ttctgaactc tttcttccca ggcgagtctg
    10501 agtatatgaa agacgcgcat ttgttatcat catccctgaa ttcagagatg aaattttggc
    10561 cactcacgag tggccttttt cttttctgtc aggcgtgttt ttccagccac accgcaaacg
    10621 gttcggtatc agcggtttcc atttcctgct gacggcgttc agatgcctcg cgctcggcta
    10681 caaaatcctc ttcgcgcaga atttccaacg gctcttcacg cagcagatta cggtaggctt
    10741 ctgcaaacgc tttgcctgtt ccgccaatac cagtatcaat catagacctt aagatacggg
    10801 cagagaaagt cagatcggga ttatcgaagc aggcgaccag ttcatcacac actttctgat
    10861 acgctttgcc gccgttaatg ctatccaacg tttgcgcgac gcgtttcaga tcgcggaaca
    10921 gatctttacc cacctgcggt aacgggaact gcgcggtttc gcagccgata cccagcgtca
    10981 ggcccggttt gcgaccttcg aggatcaccc ggttccagtt aacgcgtgta caggcaagtt
    11041 cgctactgct catttccggt gcatcagcca gcgcacacca gaccataaac aggtcgagga
    11101 atcgcacctg ctgttcatct acaccaatcg gcgagaacgg gttgatgtcc agcgaacgca
    11161 cttcaatata ttcaatgccg ccacgtaaca gcgcatcaga aggtgactcg ccgctgcggg
    11221 taacgcgttt tggacgaatc ggcgcataca gttcgttttc aatctgcagc acgttgctat
    11281 tgatttgcag cctcttaccg tctttctcaa taccaatctt cgcgtactct tccgatggcg
    11341 ttttgattgc ctgtttaagg cccgctacgt actcgtaaag atcgttgaag gtaataccaa
    11401 gattgctttg cgatttattg gtatagccga gatcgctcaa acgaagagag gtcgcatacg
    11461 gcaggtaata cataccgcac tcggttttct caaacggcag cgacgttggt tttccttgca
    11521 ggaaagaaga acaaatcgcc ggagatgcac caaacagata aggaatgacc caaccgaaac
    11581 gatagtaatt gcggataacg cggaaatagc ccgcagaaat tttctctttg gcatcagtgc
    11641 ccgagatatc accgcacttc gcttgccaga atgccattgg caaagagaaa ttgtagtgca
    11701 cgccggaaat ggtttgcatc agcgcgccgt agcgattttt cagcccttca cgatacagcg
    11761 ttttaaagcg tccggtgtta gaagtgccgt actgtgccag ttcgatgtcc tgaccttctg
    11821 cgatgtagca tggcatactt aacggccaca tccgctcatc gcccatattg cgcgccgtat
    11881 aacgatgcag atcgcgcata aaggtcaaca tatgttcaat atcaccatcc actggtgtaa
    11941 tgaattccag caatgcttcc gcaaaatcgg tagtaatcca tttgtgcgtc agtgcggaac
    12001 ctaatgcttc aggatgacct gttgttgcct gtgtgccatc agcattaaca cgcaaagttt
    12061 cgcgctccag cccacgctgt atccccttta acgcctgagg atgtttttcc agccaggcca
    12121 gcgcctgtga tacgtccggg atcaaattga cctcccgcct gtcaaaatcg ttttaattag
    12181 cataactgta atggtgacca tatgtgcagg cctacaatta gtgccaccac atcatgccct
    12241 gaacggtagc tgctgcaact gcaacatagc gtaaagcttt accaaggcac aaaaaaaaga
    12301 ttaccggtcc ccacgagatg cgcatccatc ccgctaacag acacagtaaa tcgccaacca
    12361 cgggcatcca gcttaataat agcgtgactg caccatagcg tttcagccag ccagtagctt
    12421 tctcttgcca gcgcgatgtt ttacgcaatg gaaagaaacg cccaaggata acgttagtta
    12481 accctccaag gctattaccc attgttgctg ttaaaactaa aacccaggga taactgatcc
    12541 cggaaagcaa cattgccacc agcacgactt cggagttgcc gggtaatagt gtagcgctga
    12601 gaaaactact ggcaaacaac gagaaaagcg ataacgcttc actcacagca agcgaacatc
    12661 cacggcgtcc atgcctgctg cacgggccgc ctgaataccg aaatcggcat cttcaaagac
    12721 cacacactgc gtcggttgca cgcccatacg ctgcgcgcac aacaaaaatg tgtctggcgc
    12781 gggtttatgg tgtttgacgt gatcagcagc gacgacggcg tcaaaataac ggcgtaatcc
    12841 caggtgcgcc agcaatgcct cagcgatggc gctttcactc cccgttccta cagccattgg
    12901 gcgacgacca tgccaacttt tcaccacttc aacaagagga agcggctcga cactatccag
    12961 cagcatactt cttactgctt ctgttttttc acgcgctaac gcatgcgggg cgagatcggc
    13021 ctgattcagc tcaataattg cctgagcaat acgccaggtg ggcgatccat taagcgcaat
    13081 cattgcctga acatcgtact gaagaccgta gtgccctaat acttcgcgcc acgctttacg
    13141 gtgcgtaggc tccgtatcca ggattgtgcc atccatatca aaaattaaac ctgcataacg
    13201 ctcgtacatg gtcttctcgc aagtcgaaaa atatgacgct actttagcgt aattgcttga
    13261 ttttgtcgct gatggcgaag gagaagaaaa ttcaggaaat ggataaagta gttatttaga
    13321 aatgagatat ttttgaagga aatttttggg agaagatggt gcatccggga ggattcgaac
    13381 ctccgaccgc tcggttcgta gccgagtact ctatccagct gagctacgga tgcatcggga
    13441 aaatttattt tactgctgat attgatatcg ctactaaagc catatcaagt aagagatggt
    13501 gcatccggga ggattactcg ccttgcggct cgcccttcgg gccgttgcta aagcaacgtt
    13561 atcatcccta gtgcctgcgg ttaattccgc tacctcttcg ataccttcat tgctgaagat
    13621 ttcggagaat atggtgcatc cgggaggatt cgaacctccg accgctcggt tcgtagccga
    13681 gtactctatc cagctgagct acggatgcat cgggaaactt actttactgc agattttttg
    13741 ataccgctac taaagccgta tcaagtaaga gatggtgcat tcgggaggat tactcgcctt
    13801 gcggctcgcc cttctggccg ttgctaaagc aacgttatcc tccctggtgc ctgcggttat
    13861 accgccacct cttccatacc ttcatcgctg aaagtacgta gaatatggtg catccgggag
    13921 gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat
    13981 gcaaatggcg gtgaggcggg gattcgaacc ccggatgcag cttttgaccg catactccct
    14041 tagcagggga gcgccttcag cctctcggcc acctcaccac acgcctctta cgagtgcttc
    14101 gaagaacttg tttatcgctc atcgtcgctg cgtggcgcac atattacttt ctgagactta
    14161 taagtcaaac aatttttccc acacttttat cgtttgcaca cttcacgctc aattagtctg
    14221 taaaaacgtc aaaaagagtg ttttatctac agaaaaacgg aggtctggca gatagcagta
    14281 atgcaaaaaa atggagactt aagttgaatg aacgggagta aagcgaaaag actatagagt
    14341 gaaggagaaa ttttgagggt gcgtctcacc gataaagatg agacgcggaa agattagtaa
    14401 ctggactgct gggatttttc agcctggata cgctggtaga tctcttcacg gtgaacagaa
    14461 acttccttcg gggcatttac gccaatacgt acctggttgc ccttcacccc taaaactgtc
    14521 acggtgacct catccccaat catgagggtc tcaccaactc gacgagtcag aatcagcatt
    14581 ctttgctcct tgaaagatta aaagagtcgg gtctctctgt atcccggcat tatccatcat
    14641 ataacgccaa aaagtaagca atgacaaaca cattacatgt aagcagtcat ggcattacat
    14701 tctgttaaac ctaagtttag ccgatataca caacttcaac ctgactttat cgttgtcgat
    14761 agcgttaatg cgaatgccgt gaagcgagtc cacggcattg cctgacgctt atattattgc
    14821 aatttcgcgc tgacccagcc tttcacactg gctaacgctg caggtaaggc cgcagcatcc
    14881 gtaccaccgg cttgcgccat gtcaggacgt ccaccaccct tgccgcccac ctgctgagcg
    14941 accataccaa tcagttcccc tgctttcaca cgatctgtga cgtccttaga tacgcctgca
    15001 atcagagaaa ccttaccttc ggctaccgtt gccagcacga taattgtcga ccccagctga
    15061 ttttttaaat cgtcaaccat ggtacgcaac attttcggct caacaccgct aagctcgcta
    15121 accaacagct taacaccatt aacatcaatt gccttactgg aaagatttgc gctctcctgt
    15181 gcggcagctt gttctttaag ctgttgtagt tctttttcta actgacgcgt acgttccagt
    15241 actgagcgca ctttatccgc cagattattg ctatcgcctt tcagcagatg cgcgacttcg
    15301 cttaagcgat cgctgtctgc atgaacggtg gtgatagcgc cttctccggt taccgcttcg
    15361 atacgacgaa cgcctgcagc agtacccgat tcagagatga tgcggaacag accaatatca
    15421 ccagtgcggc tggcgtgagt accgccacac agctcggtgg agaaatcgcc catgctcagc
    15481 acgcgtacac gctcatcata cttctcgccg aacagcgcca tcgcaccttt cgctttcgcc
    15541 acttcgagat ccatgatgtt ggtttcgatc ggcaaattgc gacgaatctg tgcgttcacc
    15601 aggtcttcga ccgcacgaat ctcttccggt ttcatcgctt cgttgtgtga gaagtcgaag
    15661 cgcagcacct tgtcgttaac cagtgaacct ttctgcgata catgagtccc cagaacctgg
    15721 cgcagcgcag cgtgcatcag gtgcgttgcg gagtgattca gacgaatacg ggcgcgacga
    15781 gcctcatcaa catcagcctg cacagcgtcg cccactttca gagaacccgc agcaagttta
    15841 ccgatgtgac caatcgcctg gccgtatttc tgcgtatctt ccaccgcgaa ggagaagtta
    15901 gcgtctttca gttcgccttt atcaccaacc tgaccgccgg attccgcata gaatggtgtt
    15961 tgatctagca cgaccacagc ttcctggcct gcattgatgg catcaaccgc tttaccatca
    16021 acaaacagcg cggtcacttt gccgttcagt tccagatggt catagccttt aaattcagat
    16081 gcactatcaa cacggatcat tgcgttgtaa tcggcaccaa agccgctggc ttcgcgcgcg
    16141 cgacgacgct gctcttccat tgctgcttca aaaccagctt cgtcaacttt gatgttgcgc
    16201 tcacgacaaa catcagccgt caggtcaacc gggaagccat aggtgtcgta cagacggaaa
    16261 gccgtttcac catccagcgt atcaccagaa agttttgcca gctcttcatc cagcaacgcc
    16321 agaccgcgct ccagagtacg ggcaaactgc tcttcttcag tcttcagcac ctgctcaacc
    16381 tgcgcctgct ggcgtttcag gtcttcaccc gcagagccca taacgtcgat cagcggacca
    16441 accagtttgt agaagaaggt ttctttcgcg ccgagcatat tgccgtgacg cactgcgcga
    16501 cgaatgatac gacgcagtac ataaccacgg ttttcattgg acggcattac gccatccgcg
    16561 atcaggaacg cacaagaacg aatgtggtca gcgattacgc gcagcgattt attgctcaga
    16621 tcggtcgcgc cagtgacttt cgctaccgcc tggatcaacg tgcggaacag gtcgatgtca
    16681 tagttagagt taacgtgttg cagcaccgca gcaatacgct ccagacccat accggtatct
    16741 acagacggct tcggcagcgg ttccatcgtg ccatctgcct ggcggttgaa ctgcatgaag
    16801 acgatgttcc agatctcaat gtagcggtcg ccgtcttctt ccggacttcc cggagggcca
    16861 ccccaaatgt ggtcgccgtg atcgtagaag atttcggtgc acgggccgca cggaccagtg
    16921 tcacccatct gccagaagtt gtcagatgcg tatggcgcac ctttgttatc gccgatgcga
    16981 ataatacgtt cgcgcgggat cccgacttct ttttcccaga tttcgtaggc ttcgtcgtcg
    17041 ctttcataga cggtaaccca cagacgctct ttcggcaggg caaaccattt ttcgctggtc
    17101 agcagttccc atgcaaactg aatggcatcg tgtttgaaat agtcgccgaa gctgaagttg
    17161 cccagcattt cgaagaaggt atggtgacgc gcggtgtaac cgacgttttc caggtcgttg
    17221 tgtttaccac ccgcacgcac gcagcgttgg gaagtggtag cgcgggaata attacgcttg
    17281 tcgagcccaa ggaacacatc cttgaactga ttcatcccgg cgttggtaaa caacaaagtt
    17341 gggtcgttat ggggtaccag ggagctgctg gcaactacct gatgtccctt actatggaaa
    17401 aagtcgagaa acgcctgacg gatctcagcg gtgctcttgc tcataattat cctgaaatca
    17461 agctaacgaa atatcgccac cagctccagc gtgtcttaac cgccgggctg gtaactgaaa
    17521 agtgggaata agataagttt tcttgactgg gaagtaaaat accgtatgcg ttcagtcggc
    17581 aaaatttcgc caaatatcct ggatatcttc catcagatag ccacgataga gcagaaaacg
    17641 ctggatctta actttttctg aaaagacagt tggcaaaggt tcatcatatt ttcgcgtcgc
    17701 ctgatcgcgc gccagtgcgc accagtcgat gtcacattca cgcatcgctt tttctgtcgc
    17761 ttcgcgggaa atacctttct gattcagttc ctgacgaata cgtgcaggtc cataaccttt
    17821 gcggctacgg ctggcgataa agcgcgcaac aaatcggctg tcatcgagat agccatgttc
    17881 atggcaccat gcaataacgc gctcgtaatc ttctgccgta gcatcaatct cttctgggcc
    17941 atttttgccc ataatcggtg ccgcgagttt acgtcgcagt tcttgctcac tgtgatcgcg
    18001 caccgccaga atgcgtaccg cacgatccaa caggcgagca tatgccgggc gacgggacgt
    18061 tgattctgtc atggcatatc cttacaactt aaaaaagcaa aagggccgca gatgcgaccc
    18121 aggttttgct gataaacaga tttaatagtc aatgagtgtg cagaatgttc ccgcaggccc
    18181 aattcgttag aggatcgagg tgagagtaat ttttcacgcc agtgctggcc tgttcccctc
    18241 accctaaccc tctccccaaa ggggcgaggg gaccgtccgt gcacacatta tctattatca
    18301 tcaataacga acgattaaaa atcttcgtta gtttctgcta cgccttcgct gtcatctaca
    18361 gagaaatccg gcgttgagtt cgggttgctc agcagcaact cacgtacttt cttctcgatc
    18421 tctttcgcgg tttccgggtt atctttcagc caggcagttg cattcgcttt accctgaccg
    18481 atcttctcac ctttgtagct gtaccacgcg cctgctttct cgatcagctt ctcttttacg
    18541 cccaggtcaa ccagttcgcc gtagaagttg ataccttcgc cgtagaggat ctgaaattca
    18601 gcctgtttaa acggtgcagc gattttgttc ttcaccactt tcacgcgggt ttcgctaccc
    18661 accacgtttt cgccctcttt caccgcgccg atacgacgga tgtcgagacg aacagaggcg
    18721 tagaatttca gcgcgttacc accggtagtg gtttccgggt taccgaacat cacaccaatt
    18781 ttcatacgga tctggttgat gaagatcagc agcgtgttgg actgcttcag gttacccgcc
    18841 agcttacgca tcgcctggct catcatacgt gccgcaaggc ccatgtgaga gtcgccgatt
    18901 tcgccttcga tttccgcttt cggcgtcagt gccgccacgg agtcaacgac gataacgtct
    18961 actgcaccag aacgcgccag ggcgtcacag atttccagtg cctgctcgcc ggtgtccggc
    19021 tgggagcaca gcaggttgtc gatatcgacg cccagtttac gtgcgtagat tgggtccagc
    19081 gcgtgttcag catcgataaa cgcacaggtt ttaccttcac gctgcgctgc ggcgatcacc
    19141 tgcagcgtca gcgtggtttt accggaagat tccggtccgt agatttcgac gatacggccc
    19201 atcggcagac cacctgcccc aagcgcgata tccagtgaaa gcgaaccggt agagatggtt
    19261 tccacatcca tggaacggtc ttcacccagg cgcatgatgg agcctttacc aaattgtttc
    19321 tcaatctggc ccagtgctgc cgccaacgct ttctgtttgt tttcgtcgat agccattttt
    19381 actcctgtca tgccgggtaa taccggatag tcaatatgtt ctgttgaagc aattatactg
    19441 tatgctcata cagtatcaag tgttttgtag aaattgttgc cacaaggtct gcaatgcata
    19501 cgcagtagcc tgacgacgca ccgcatcacg gtcgccgctg aagcattccc gccgggtaat
    19561 gccttcaccg cgggcagtgg caaaagcaaa ccagacggtg ccgacaggct tctcttcact
    19621 gccgccatcc ggcccggcga taccactaat agacacggca taatcagcac gagccgcttt
    19681 cagtgcgcct atcaccattt ccaccacgac gggttcactc accgcgccat gctgcgccag
    19741 cgtctcttcg cgtacgccga tcatctgcgc tttggcttcg ttactgtagg tgacaaatcc
    19801 gcgttcaaac caggcggagc taccggcaat atcggtaatc actttcgcta cccaaccacc
    19861 ggtacaagac tcggcagttg ttacggttgc gccacgggct ttcagcgcct gcccaacctg
    19921 ttcacttaac tgcatcagtt cactgtcagt catcacaacc tctgtcagtc aaaaatttat
    19981 gccggacaag atagcacttt cgtcaggaag atggtgttgg cgttgcaatt ttacgaggag
    20041 gattcagaaa aaagctgatt agccagaggg aagctcacgc ccccctcttg caaatagtta
    20101 ctgcacgcgc gccagcgcca cggcttgtcc taactgccag accgccattg cgtaatgggt
    20161 gctgtggtta taacgggtaa tggtgtagaa gttcggcaga ccgtaccagt actggtaacc
    20221 ggtaccgaca tccagacgca gcaggctggc ttgttgatgg ttgcccagcg gctgctgtgg
    20281 cgttaaacct gccgtggcaa gctgcgaaat gctgtacctg gttttgaagc catttggcaa
    20341 gcctggagcc tgaccgtttg ccattaccgc gacctgatcg cctttcaccc agccgtgcgc
    20401 tttgaaatag ttcgccacgc taccgatcgc atcaaccgga tcccacaggt tgatatgccc
    20461 gtcgccgctg aaatctaccg catattgttt gtaagacgac ggcataaact gtccgtagcc
    20521 catcgccccg gcaaaggaac ctttcagatt gagcggatcg tcctgctcgt cgcgcgccat
    20581 cagcaggaag gtttccagct cgccagagaa atactccgcg cggcgtgggt agttaaatga
    20641 cagcgttgcc agcgcatcga ggatgcgagt tttccccatc acgcgccccc agcgggtttc
    20701 aacgccgata atcccgacga taatttccgg cggtacgcca tacacctgcc acgcgcgatt
    20761 caacgcatct tcatactgat tccagaaaac cacaccgttc tgcacgttgt ccggcgtaat
    20821 aaattttttg cgataacgca gccatgcgcc gttcggacct gatggcggtt tcaccgatgt
    20881 ggttggtgcc tggttatcca tcagccgcag taccgaatcc agacgcttcg cctgggagag
    20941 aatttcctgc aactgctgac gatcgaaacc gtgtttgttc accattttgt cgatgaactg
    21001 ctgggcattc gggttattag cgaaatcgcc gcccatctgc atcacattgt gctgcggctc
    21061 aagcaggaag ccgccagacg gcgttccggt ggtcgtttca gtctcagtag gttttggctt
    21121 gctgctacag gcggcaagca acacaaaaag gggaagcaat gttacataac gacgcttgaa
    21181 catgaggggt ccatttaaca gattcaacca ggggcaagta tggtaaagca tcacgccccg
    21241 cacaaggaag cggtagtcac tgcccgatac ggactttaca taactcaact cattcccctc
    21301 gctatccttt tactcaaact tccaaattaa aatatttatc tttcattttt agattaaaat
    21361 aacactttta aatctttcaa tctgattaga ttaggttgcc gtttggtaat aaaacaataa
    21421 atcctgaagg agagaacaat gatagaaacc attactcatg gtgcagagtg gtttatcggg
    21481 ctgttccaaa agggcggaga ggtgtttacc gggatggtga ccggcattct tccgctgttg
    21541 attagcctgc tggttatcat gaacgcactg attaatttta tcggtcagca tcgtattgaa
    21601 cgttttgctc aacgttgcgc cggtaaccct gtttcccgtt acctgctgct accgtgcatt
    21661 ggcacgtttg tcttttgcaa tccgatgacc ttaagccagg gtcgctttat gccggaaaag
    21721 tacaaaccca gctactacgc ggcggcctct tatagctgcc actcaatgaa tggcctcttt
    21781 ccccatatca accctggcga actgtttgtt tatcttggca ttgccagcgg tctgacaacg
    21841 ctgaacctgc cacttggccc actggcgttg agctatctgc tggttggtct ggtcaccaac
    21901 ttctttcgcg gctgggtgac cgatctgacc accgccattt ttgagaaaaa gatgggcatt
    21961 caacttgaac aaaaagttca cctggcagga gcaacatcat gacgcgtatt cggatcgaaa
    22021 aaggaacggg tggctggggc ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg
    22081 tctatatcac cgccggtacc cggcctgcga ttgttgacaa actggcacaa cttactggct
    22141 ggcaggctat tgacggattt aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg
    22201 taatcgactg tggcggcaca ttacgctgcg gcatctatcc gaaacgacgt attcccacca
    22261 ttaatatcca ctcgacgggc aagtccggtc cgctggcgca gtacattgtg gaagatattt
    22321 atgtctctgg cgtaaaagaa gaaaacatca ctgtagtggg tgatgcgaca ccacaaccct
    22381 cttccgtggg ccgtgactat gacaccagta agaaaatcac cgaacaaagc gatggtttac
    22441 tggcgaaggt gggaatgggc atggggtccg ccgttgcggt gctgtttcaa tctggtcgtg
    22501 acaccatcga cactgtatta aaaaccattc tgccgtttat ggcattcgtc tcggcgctca
    22561 ttggcatcat tatggcttct ggccttggtg actggattgc ccacggtctt gctccgctgg
    22621 cgagccatcc actgggtctg gtcatgctgg cgctcatctg ctccttccca ctgctttcac
    22681 ctttcctcgg cccaggcgca gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga
    22741 ttggtctcgg caatattccg ccgcatctgg ctttaccggc actgtttgcc atcaacgcgc
    22801 aggcggcctg cgacttcatc ccggtcggtt tgtcgctggc ggaagcccgt caggacacgg
    22861 ttcgcgtcgg tgtcccttct gtactggtga gccgcttttt aaccggcgca ccgactgtac
    22921 tgatcgcctg gtttgtctcc ggttttatct atcaatagag gctgaaacat gaccgttatt
    22981 tataagacca ccatcacccg tatcggcgcg agtgccactg acgccctcag cgaccagatg
    23041 ctcatcacct ttcgtgaagg cgcgccagcg gacctcgaag agtattgctt cattcattgc
    23101 cacggcgagt tgaaaggtgc actccatccc ggtttgcaat ttacgctcgg gcagcatcgc
    23161 tatccggtga ccgctgttgg cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc
    23221 accctgcgct tcgatggttt aaacgaagcg gaatttccgg gcactgtcca tgtggcaggc
    23281 cctgtacccg acgatatcgc gccgggatcg gttttgaagt ttgaatctgt taaggagtaa
    23341 aaaatgaatc aggttgccgt tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc
    23401 cacggtctgg ttgccgaggg gtatcgcgtc gcggttgtcg atattcagag cgacaaagcc
    23461 gcaaatgtgg cacaagaaat taacgccgaa tatggtgaaa gtatggcgta cggttttggt
    23521 gctgacgcca ctagcgagca aagcgttctg gcgctctctc gtggggtaga tgaaatcttt
    23581 ggtcgcgtgg atttgctggt ctatagcgcc ggaatagcaa aagcagcctt tatcagcgac
    23641 ttccagctcg gcgattttga ccgttcgcta caagtgaatc tggtgggtta tttcctgtgt
    23701 gcccgtgaat tttcgcgttt gatgatccgc gacgggattc agggtcgcat tattcagatc
    23761 aactcgaaat ccggcaaagt gggcagcaaa cacaactctg gctacagcgc agcgaaattt
    23821 ggtggcgtcg ggctgactca atcactggcg ctggatctgg cggagtacgg cattacggtg
    23881 cattcactga tgctcggtaa cctgctgaaa tcgccgatgt tccagtcact gttgccacaa
    23941 tacgcgacca agctgggtat caaaccggat caagtcgagc agtattacat tgacaaagta
    24001 ccgctcaaac gcggctgcga ttatcaagat gtgctgaata tgctgctgtt ctacgccagt
    24061 cctaaggcgt cgtactgcac cggacagtcg atcaatgtca ccggcggtca ggtgatgttc
    24121 tgatcaacag cggagatcca ttaaggatct ccgtgagact atagaatgcc tgatgcgcta
    24181 cgctcatcag gcatacagga cttccgccac tacattaagg aaaagttatg gtatccgcac
    24241 tcatcaccgt cgccgttatc gcctggtgtg cgcaactggc cttaggcggc tggcaaattt
    24301 ctcgttttaa ccgtgccttc gacacactat gccagcaagg gcgggttggc gtgggtcgtt
    24361 ccagcgggcg ctttaaaccg cgggtcgtgg tcgccatcgg ctggacgatc agcagcgcat
    24421 cgtcgacacc ttgtttatga aaggactgac cgtcttcgcc cgaccgcaaa aaattcccgc
    24481 aattaccggt atgcatgcgg gtgatttaca gcccgatgtg atctttcccc atgatccact
    24541 atcacagaat gctctatcat tggcgcttaa actgaaacgt ggataatttc gttgtgaatg
    24601 ttacttgctt gcgaagttat cattttgaaa cctaaatcag gtaatcacgc ccatgaaacc
    24661 tcgtcagcgt caggccgcca ttctggagta tctgcaaaag cagggtaaat gctcggttga
    24721 agaattggcg caatactttg acaccacagg cacaaccatt cgcaaagatc tggtcattct
    24781 ggaacatgcc ggaaccgtca ttcgtactta tggcggagtg gtgttgaata aagaggaatc
    24841 cgatccgcct atcgatcata aaacactcat caacacccac aagaaagagc tgattgcaga
    24901 agctgccgtt agttttatcc atgatggcga ttcgatcatt cttgatgctg gcagtaccgt
    24961 tttgcagatg gttcccctgc tctcgcgctt taataacatc acggtgatga ccaacagcct
    25021 gcatatcgtc aatgcgctat ccgaactgga taacgaacaa actatcctga tgccaggcgg
    25081 aacgtttcgt aaaaaatcgg cctcatttca cggacagctg gcagagaatg ccttcgaaca
    25141 tttcagtttc gataaattgt ttatgggcac cgacggcatc gatctcaatg cgggcgtaac
    25201 caccttcaac gaggtttata ccgtcagtaa ggcaatgtgc aatgccgcgc gcgaagtgat
    25261 tttgatggcg gactcatcaa agtttggccg taaaagcccc aacgtagttt gcagtcttga
    25321 aagcgtcgat aagctgatta ccgacgcagg tatcgatccg gcgtttcgtc aggcgctgga
    25381 agagaaaggg atcgatgtga tcataaccgg agagagcaat gagtgaagca ctactgaacg
    25441 cgggacgtca gacgttaatg ctggagttgc aggaagcaag ccgtttaccg gaacgtctgg
    25501 gcgatgattt tgttcgcgcc gccaatatca tcctgcactg cgaaggcaaa gtggtggttt
    25561 cgggaattgg caaatcgggc cacattggta agaaaatcgc cgcaacactt gccagtaccg
    25621 gcacaccggc tttttttgtc catccggcag aagcactgca cggcgatctg gggatgattg
    25681 aaagccgcga tgtgatgctg tttatctctt actccggtgg cgcgaaggaa ctggatctga
    25741 ttattccgcg tctggaagat aaatctatcg cgctgctggc gatgaccggc aaaccgacgt
    25801 caccgctggg cctggcggca aaagcggtgc tggatatctc cgtagaacgc gaagcctgcc
    25861 cgatgcacct tgcgccgacc tccagcaccg tcaataccct gatgatgggt gacgcgctgg
    25921 cgatggcggt catgcaggcg cgcggattta atgaagaaga ttttgcccgc tcccacccag
    25981 ccggggcact gggcgctcgc ttgctgaata aagtgcatca tctgatgcgc cgtgacgatg
    26041 ccatcccaca ggtggcgtta accgccagcg tgatggatgc gatgctggaa ctcagccgca
    26101 ccggtctggg gctggtggcg gtatgtgacg ctcaacaaca ggtacaaggc gtctttaccg
    26161 acggcgattt acgtcgctgg ctggtaggcg gcggcgcact caccacgcca gttaatgaag
    26221 cgatgacgac aggcggcacc acgttacagg cgcaaagtcg cgccatcgac gccaaagaga
    26281 tcctgatgaa gcgcaaaatc actgccgcac cggtggtgga tgaaaacggc aaactcaccg
    26341 gcgcaattaa cctgcaggat ttctatcagg ccgggattat ttaatccttc attcccagac
    26401 gtttcgccag ccgatgcagg ttggcgacgt cggtttccag catccgcgcg caggcagccc
    26461 agttatgatg attttgtgcc agtgcctggc gaatagtttc acgctggaac gcttctgtcg
    26521 cttcacgcag gttttgctta acaacgggca ccgccgccgc ttctggcggc ggcaacgtca
    26581 cctcaggaaa agcaaaatgt tgcgcctcaa gaatcacttc atcgccgttg cgggtggctc
    26641 tcgccagcac taccgcccga tgaatagcat gttccagttc gcgcacgttc cccggaaaac
    26701 gatagtgttg cagtaaattt cgcgctccgg cacttaatac cacgcgggag agccccagcc
    26761 gcaaacgaca ctgttcgcag aaataccccg ccagcagaat gacatcatcg ccccgctcac
    26821 gcagcggcgg caccgaaagt ggaaacacgc tcaggcgatg aaacaagtca gcgcgaaatc
    26881 gccctgccag cacctcttcg cgtaagtcgc ggttagtcgc cgccagcacg cgcacatcga
    26941 cccgcaaact acggtcatcg ccaacgcgct gaatatcgcc atactgcaac accctcagca
    27001 gcttggcctg caatgccaac gacaactcgc cgatctcatc cagaaacaga gtgccgttat
    27061 ccgccatttc aaacttcccg ctgcggttac tgatagcgcc agtaaacgct cctttcacat
    27121 gcccgaacaa ctcactttcc gccacacttt ccggcagtgc agcacagttg agatagacca
    27181 gcggattcac cgcccgtggc gaggcttcat gaatcgcttt cgccaccagc tccttaccgg
    27241 ttcccgtctc accgctgatc aggacgttga gatcggacgc cgccacaatc tcaatctctt
    27301 ttttcagttg cgtcatgcca ggggacaagc caatcatctg cgtctgtttc accgcttcaa
    27361 acggcgtggc atcgcctggc atcatattct ggctttccag ttgttcaatc agcaacgcat
    27421 tgcttaacgc tcccgccgcc agcgcagcaa tcagccgtag ctcttcgtcg ctgaaaacat
    27481 cgaactgatc gggctgcatc ccgtcgagcg tcagtgcgcc gatcaggttt tgcccggcaa
    27541 acaatggcag accaacgcag gcgtgaacct tcagactctc ctgcccagga atcaaaccgt
    27601 catagggatc gggcaattcg ctgtctgcgg gaaagcgcac cacatccccg gcgcgggcaa
    27661 tcgcttccag ccgtggatgc ccttccagcg caaagcgtct accgagtaca ccctttgcca
    27721 gaccgtcgat ggcaagcgga ataaactgcc gcgaatcgta acgtagcaac gcagacgcat
    27781 cgcactccag cacctgacgt agcgtggtga tcaggcgctg aaaacgatcc tgatgaccaa
    27841 tcccacgctg caattcgatg gcgatattcg ccagcacatc aacggaaaaa ctcatctttg
    27901 cctcactgtc aatttgacta tagatattgt catatcgacc atttgattga tagtcatttt
    27961 gactactcat taatgagcat aattttattt atagagtaaa aacaatcaga taaaaaactg
    28021 gcacgtaatc tgcaattagc aagacatctt tttagaacac gctgaataaa ttgaggttgc
    28081 tatgtctatt gtggtgaaaa ataacattca ttgggttggt caacgtgact gggaagtgcg
    28141 tgattttcac ggtacggaat ataaaacgct gcgcggcagc agttacaaca gctacctcat
    28201 ccgcgaagaa aaaaacgtgc tgatcgacac cgtcgaccat aaattcagcc gcgaatttgt
    28261 gcagaacctg cgtaatgaaa tcgatctggc ggatatcgat tacatcgtga ttaaccatgc
    28321 agaagaggac cacgctgggg cgctgaccga actgatggcg caaattcccg atacgccgat
    28381 ttactgtact gccaacgcta tcgactcgat aaatggtcat caccatcatc cggagtggaa
    28441 ttttaatgtg gtgaaaactg gtgacacgct ggatatcggc aacggcaaac agctcatttt
    28501 tgtcgaaacg ccaatgctgc actggccgga cagcatgatg acttacctga caggcgacgc
    28561 ggtgctgttc agtaacgatg ctttcggtca gcactactgc gacgagcatc tgttcaacga
    28621 tgaagtggat cagacggagc ttttcgagca gtgccagcgt tactacgcca atatcctgac
    28681 gccgttcagc cgcctggtaa caccgaaaat taccgagatc ctgggcttta acttaccagt
    28741 cgatatgata gcgacctctc acggcgtggt atggcgcgat aacccgacgc aaattgtcga
    28801 gctgtacctg aaatgggcgg ctgattatca ggaagacaga atcaccattt tctacgacac
    28861 catgtcgaat aacacccgca tgatggctga cgctatcgcc caggggattg cggaaaccga
    28921 cccacgcgtg gcggtgaaaa ttttcaacgt cgcccgaagc gataaaaacg aaatcctgac
    28981 caatgtcttc cgctcaaaag gcgtgctggt cggcacttcg acgatgaata acgtgatgat
    29041 gccgaaaatc gccgggctgg tggaggagat gactggttta cgcttccgta acaaacgcgc
    29101 cagtgctttc ggctctcacg gctggagcgg cggtgcggtg gatcgtcttt ccacgcgcct
    29161 gcaggatgcg ggtttcgaaa tgtcgcttag cctgaaagcg aaatggcgac cagaccagga
    29221 cgctctggag ttatgccgtg aacacggtcg cgaaatcgcc cgtcagtggg cgctcgcgcc
    29281 gctgccgcag agcacggtga ataaggtagt caaagaagaa acctctgcca ccacgacggc
    29341 tgacctcggc ccacggatgc agtgcagcgt ctgccagtgg atttacgatc cggcaaaagg
    29401 cgagccaatg caggacgttg cgccaggaac gccgtggagt gaagtcccgg ataacttcct
    29461 ctgcccggaa tgctccctcg gcaaagacgt ctttgaagaa ctggcatcgg aggcaaaatg
    29521 agtaacggca ttgtgatcat cggttcgggc ttcgccgccc gccaactggt gaaaaatatt
    29581 cgcaaacagg acgccactat tccattaacc ctgattgccg ccgacagcat ggatgagtac
    29641 aacaaacctg acctcagcca tgttatcagt caggggcaac gtgccgatga ccttacccgc
    29701 cagacggcgg gtgaatttgc cgagcagttt aatctgcacc tgtttccaca aacctgggtg
    29761 acggatatcg atgccgaagc ccgtgtggtg aaaagccaga ataatcagtg gcaatacgac
    29821 aagctagtac tggcaaccgg tgccagtgcc tttgtcccgc ctgagcctgg gcgtgagtta
    29881 atgctgacgt taaatagtca gcaagagtat cgcgcctgtg aaacgcaact gcgggatgcc
    29941 cgacgcgtgt tgattgttgg cggtggtttg attggtagcg aactggcgat ggatttttgt
    30001 cgtgcaggca aagcggtcac gctaatcgac aacgctgcca gtattctggc gtcgttaatg
    30061 ccaccggaag taagcagccg cttgcagcat cggttgacgg agatgggcgt tcatctgctg
    30121 ttgaaatctc agttacaggg gctggaaaaa acggattctg gcattctggc aacgctggac
    30181 caccagcgta gtatcgaagt ggatgcggta attgccgcca ccggactgcg cccggaaaca
    30241 gccctggcac gacgcgccgg gctgacgatt aatcgcggcg tttgcgtcga tagttatctg
    30301 caaaccagta atgacgatat ttatgcgctg ggcgattacg cggaaatcaa cggtcaggta
    30361 ctgccattcc tgcagccgat tcaacttagc gcgatggtgc tggcaaaaaa tcttctcggc
    30421 aataacacgc cgctgaaact accgacgatg ctggtgaaaa tcaaaacgcc ggaattaccg
    30481 ctgcatctgg caggcgaaac ccagcgtcag gatttacgct ggcaaattaa taccgaacgc
    30541 tagggaatgg tggctcgcgg tgttgacgat gctgaccagc ttcgcgcctt tgtggtcagt
    30601 gaggatcgga tgaaagaggc atttggattg ttgaaaacat tgccgatgta ggtgggctac
    30661 tgtgcctaaa atgtcggatg cgacgctagc gcgtcttatc cgacctacgg ggacgcatgt
    30721 gtaggccgga taaggcgttt acgccgcatc cggcaatggt gtccaaatgc aacacgtttt
    30781 atccgttctg gacttcaccc gctaaccaac gcgccgcagc aataaccccc tgccccagag
    30841 acaaaccgcc atcacccgcc ggtaaactct gtggaaagag caatgtgaaa tcagcgagat
    30901 aatgcgccag acgtgcacgc agcaaacggt tatgaataac cccgccgcta aataccagcg
    30961 tagtgatacc acgcatcgtg gcctgctcac gcatcaacgc ggcaaaaccc tgcgccagcg
    31021 catcatgaaa cgcccacgcg cgttgattaa cctgtgcctg ccagttcagc cactgctgcc
    31081 agaaagcggc gagatccagt tgattgtcca ccagcggcat cgtcaccgga tgcgtcactc
    31141 cgtggcatga ggctgcgagc gcctccagag cacaagccgc ttcaccttca taacttaacg
    31201 tggctggcgc acagcccagt gccgccgcca ctgcatcgaa caaccgccca cacgatgacg
    31261 ccagcggcgc gttaattcca cgctcaatgg cccgcgccag cacgctccag ttttgctgtt
    31321 gcacacttgc tgtttcaggg taattctgcc actccggcac aaagcgcagg cactgcgcca
    31381 gcaggtttcg ccacggctgc ttcgctgcca aatcgccacc cggaagcgcc actgcaggca
    31441 agccgcccag gtgctcacat tcgcgatagt tcacccgcag gcactcgccg ccccacaaag
    31501 cgccgttctc ccccataccg ataccgtcga gcgtcaaagc aatgacatca ccgccatcca
    31561 gcggccactg atgctctgcc agacacgccg ctgcatgggc atgatgatgc agtaccgttt
    31621 gcgtcggcag attcatttca cgcgcccact ggctggagac atagcccgga tgcgcgtcat
    31681 gcacaacgta tttcggggta aaatcgtaga tgttttgcat caggcgtaac gcttcgcgcc
    31741 actgcatctg gatgccatcg tcacttaaat cgcccagatg ctgactcaac accgcttgtt
    31801 caccgcgcac caggcagaag gtatttttca gatccgctcc gagacacagc acaggcggaa
    31861 catttttaaa gcccggaggc aaagccagcg catccggcac atacccccgc gaacggcgca
    31921 gcatttcgcc gctttcgcgc accaccgaat catccatccg ctgcacgatg tcgcggttat
    31981 gtatcaagaa tccgtcggca atgccctgca aatccgccag cgcctgttcg ttgctgatag
    32041 ctggtggttt accgctcagg ttgccggagg tcatcaccag cgggcattgc agttcctgta
    32101 acagcaaatg ctggagcggg ttcgcaggca acattacccc gacttcgtta aggtcagggg
    32161 cgatatcatc acaaagctca ggaacgtatt ttttatccac cagcacaatc ggcgcggcgg
    32221 gcgtggtaag caactggcgc gcagcgtctg gtaaaccgtc agccactggc aacatgaccg
    32281 ccagcggttt cgccgggcga tgtttgcgcg cccgaagtgt cgccaccgcg ttactgttac
    32341 gtgcatcgca ggcaagatga aatccgccaa tccctttgat ggcgacaatg ttgcccattt
    32401 ttaactgtgc gatagccgcc tgtaatgccg cctcttgttc tgcatgttca ccatgactta
    32461 cccattcaag atgcgggcca cactccgggc aggccaccgg ctgggcgtgg aagcgacgat
    32521 cgagcgggtc gcggtactct ttgtcacaag ccggacacaa cggaaacgcc gccatcacgg
    32581 taaacgggcg gtcgtaaggc atggcgcgaa taatggtgaa acgcgggccg cagtgggtac
    32641 agttgataaa cggataacga taacgccgtt cgcctggggt attcatttcg gcaaggcaag
    32701 cagggcaagt agcggcatcc gggacaattt gcgtattcat ggcacctccc gcgctctggc
    32761 ggatggtgaa ctcagtgggc agttgtgacc agatgtacgg ctcacgctcg acgctatcaa
    32821 tacgcgccag cggcgggcag tgctgatgca attgaacaag aaacgtttcc gggtcttccc
    32881 gcagccggac ttctacgcca tcgccgtcat tacagacatc gccgtgaaga tttaattgct
    32941 gtgccagctg ccagacaaac ggacgaaaac cgacgccctg cactttgcca cgaatacgca
    33001 gttggacacc gcaagatgtg ttttttgcca ttgagttatt cccgccatca tgaattgcgt
    33061 aacccgccca gccggacacg acagcgtcgc atccggcagt cacaggtcgg cgataccgcc
    33121 cgctccgtat tctacgaata tttccgggaa ttcctttgat gccagaacag ttctgtaaga
    33181 tttttagaac atcagcgccg tacggcggcg tttttctgca ctcagttgtt caagtttatt
    33241 acgatcgaca caaatcagcg catgagtcgg gcaagccgcc atacacgccg ggccgtcttc
    33301 acgatggttg cacaggtcac atttattggc ttcggctttg tcagcccgta cattcagccc
    33361 cgcgccgctg ttgcgaatca ccggacgtac caccacttcc atcgcgccat acgggcaagc
    33421 cacaacgcag gttttgcaac caatgcaacg ttcctgcatc acatgaacaa accctttatc
    33481 acggctgata gcaccattcg ggcagacgtt agcgcacggt gcatcttcac actgacggca
    33541 aactgtcgcc gtggaaatgt tcacaccttt aatgacatgg atacgcggta aaaatgtttc
    33601 cggggtcagc gatgcacagt cctgattttc ctgatgagaa accacgcacg ccacttcaca
    33661 ggtacggcaa ccaatacatt tactcgcgtc agcaatgatg aaacggttca tcaaattctc
    33721 cagcaatgac agttaatgcg ccgatacatt cacaaatcat gccagttttt aattcactgt
    33781 tatttaagga aattaatttc tgtaatgcag gaaaaaagat gtcatcgaca ctagtgacga
    33841 tgacatgtga tgacaatgtt tatcgcgaaa gagcaatgag tgagtcgcgg tggatcagtt
    33901 ttccgctgaa ggttttcggc ggtgagaaat ccccgccatc gagcataaaa atcagccgtc
    33961 caataatttc ctgaatcatc tcagtcaccg gaatttttac gctggagagc gccggaacga
    34021 tatagggggc aattgcgata tcatcgaatc cgataactga cacctgctct ggcaccgcta
    34081 cgccgcgctc gtgcaacgct ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta
    34141 acgcgctaaa tttagcccca cgttcgagca acgtttctac cccttcggcc ccgctggcgg
    34201 gcgtccattt accgttagcg ataagttttt cattgagcgc aataccatgc tgcgccagcg
    34261 cgtctttata cccagcaaga cgttcaatgc tggtggggga atccatcgag ccggtaagga
    34321 aagcaatctc ctgatgtccg gcgtttatca actctgccac ggcattaaag ctggtctgtt
    34381 tatgatcgca ccagacgcta tggctgctgt ttttgcgcag gcggcgatta agcaccatta
    34441 tcggctgact gtgcgcgtca atgatgtcat cgatctcatc cacgcttaaa aaacgcgggt
    34501 aaatcataat ggcgtcgcag cgcaaatcca gcagatactg aatcgcctgg cgctcttctt
    34561 cggcgctgtg tttaccatct gccaatagca actgccgccc tttctcttcc gccattcgcg
    34621 cggcatgaaa gagtaattca ctaaaataaa tgccgtggta aagcgtgttg gtcactacca
    34681 gccccagcgt ctgagtactc ttcgccgaca gattgcgcgc cagcaagttt gggcggtaac
    34741 cgctctcttc taccgcctga aacacgcgat ctttagtctc ctggctgacg tagccattac
    34801 ctgaaagcac gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc acttccagca
    34861 tcgtcgtcat cattttcatc cctttacacg caatcaacgc agtgtactgc accgtttgcc
    34921 gattgtcctt gcgcaatcgg cgggaaaaat attcaggtga ccggtttcac aaatataaaa
    34981 aatgaataat taactctctt gcttatttag tgacaactat tcatgatttt gtgaaaccgg
    35041 tttcctaatt ccgtttcagc atcggcattt ttccgttacg tcgactgata acaactacat
    35101 ctaccctact gataacagga taaaatccga tggccaaaaa ttatgcggcg ctggcacgct
    35161 cggagatagc ggcactgggc ggcgttgata acatctcggc ggtcacgcac tgtatgacgc
    35221 gtttgcgctt tgttatcaaa gatgaccagc ttatcgacag cccgacgtta aaaaccatcc
    35281 ccggcgtgct cggcgtggta cgtagtgaca accagtgtca ggtgattatc ggcaataccg
    35341 tttcacaagc ctttcaggaa gtcgtcagcc tgctgccggg agatatgcag cccgcacagc
    35401 ccgtgggtaa acccaaactc acgccgcgtc gcattggtgc ggggatcctc gatgcgctga
    35461 tcggcaccat gtcaccgctg atcccggcga ttatcggcgg atcgatggtc aaactgctgg
    35521 cgatgatcct cgagatgagc ggcgtgctga caaaaggatc gccgacctta accattctga
    35581 atgtgattgg tgacggtgct ttcttcttcc tgccgctgat ggtcgcggca tctgccgcca
    35641 tcaaatttaa aaccaatatg tcgctggcga ttgccatcgc gggtgtgctg gtacatccga
    35701 gttttattga actgatggcg aaagcggccc agggtgaaca tgtcgaattt gccctgattc
    35761 cggtcaccgc ggtgaaatac acctacacag tgatcccggc gctggtgatg acctggtgcc
    35821 tgtcatatat cgaacgctgg gtggatcgca ttacaccggc ggtgaccaaa aacttcctca
    35881 agccaatgct gattgtgttg attgccgcac cgctggcaat cctgctgatt ggcccgattg
    35941 gtatctggat cggtagcgcc atttcggcgc tggtttacag cattcatggt tatctgggct
    36001 ggctttcagt cgccattatg ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc
    36061 gcgtctttac gccaaccatc attcagacca ttgccgaaac cggcaaagaa gggatggtca
    36121 tgccgtcaga gatcggcgct aacctgtcgc tgggcggttc atcactggcg gtggcgtgga
    36181 aaacgaaaaa cccggaactg cgccagacgg cgctggctgc ggcggcatca gccattatgg
    36241 cggggatttc cgaaccggcg ttatacggcg tggcgatccg cctgaaacgt ccgcttatcg
    36301 ccagtcttat cagcggtttt atttgcggcg cggttgccgg tatggcgggg cttgccagcc
    36361 actcaatggc agcgccgggg ctatttacca gcgtgcaatt cttcgatccg gcgaatccaa
    36421 tgagcatcgt ctgggtgttc gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca
    36481 cactgttgct cggctttgag gatattcctg ttgaggaagc ggctgcccag gcgcgaaagc
    36541 atcagagcgt acaaccgacc gtcgccaaag aagtaagtct taattgagga tgaaaatgtc
    36601 agtatttcca gaaggttttt tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc
    36661 gttccgtgaa ggcggaaaag ggctgaccac cgtcgatatg atcccacacg gcgagcatcg
    36721 aatggcggtg aaactggggc tggaaaaacg ttttcagttg cgcgatgacg agttttatcc
    36781 cagccatgag gcgacggatt tttatcatcg ttataaagaa gatatcgccc tgatggcaga
    36841 gatgggattc aaggttttcc gtacctcaat tgcctggagc cgtctctttc cgcagggcga
    36901 tgaaatcacg cccaatcagc agggcattgc tttttatcgt tctgtctttg aagagtgtaa
    36961 aaagtacggt atcgaaccgc tggtcacgtt gtgccacttc gatgtgccga tgcatctggt
    37021 caccgaatat ggctcctggc gtaaccgcaa gctggtggag tttttcagcc gctacgccag
    37081 aacctgcttt gaagcatttg atggtctggt gaaatactgg ctaaccttca atgaaatcaa
    37141 cattatgttg catagcccgt tctccggcgc gggtctggtg tttgaagaag gtgaaaatca
    37201 ggatcaggtg aaatatcagg ccgcgcatca ccagctggtt gccagtgcgc tagccgccaa
    37261 aatcgcccat gaggttaacc cgcaaaatca ggtgggctgt atgctggcgg gcggcaactt
    37321 ctacccttac agctgcaaac cggaagatgt ctgggcggcg ctggagaaag atcgggaaaa
    37381 cctgtttttt atcgatgtgc aggcgcgcgg cgcgtatccg gcttactctg cccgcgtatt
    37441 tcgcgaaaaa ggggtaacca tagataaagc gctgggcgat gatgagatcc tgaaaaacac
    37501 cgtcgatttt gtctctttca gctattacgc ctcgcgctgc gcctcggcgg agatgaacgc
    37561 caacaacagc agtgcggcga acgtggtgaa atcgctgcgt aacccgtatc tgcaagtgag
    37621 cgactggggc tggggaattg atccactcgg tctgcgtatc accatgaata tgatgtacga
    37681 tcgttatcag aagccgctgt ttctggtgga aaacggcctc ggcgcaaaag atgaacttgc
    37741 tgccaatggc gagattaacg atgactatcg catcagctat ttacgcgaac atatccgcgc
    37801 aatgggcgaa gcgattgcag atggcattcc gctgatgggc tacaccacat ggggctgtat
    37861 tgatttagtt tccgcctcta cgggtgaaat gagcaaacgc tacggttttg tctacgtaga
    37921 ccgtgacgac gcaggcaacg gcacgctgac gcgcacacgt aagaaatcgt tctggtggta
    37981 taaaaaagtg attgccagta atggggaaga tttagagtaa gtaacagtgc cggatgcggc
    38041 gtgaacgcct tatccggcct acatgtcgca cgaaccagga aggcccgata agcgcagcgc
    38101 atcgggcaat ttagcatttg gcatcagagc tggttcaccc catcaagaac atccctgtcc
    38161 tgattcctta atgaaaaact actcttcttc caccgctaac tgcgcgaagc cgccattccc
    38221 ttcccagcct tccagtcgtt gataaacggt ttctaccgca tctttaatcg gctgggtcat
    38281 cgggtagtaa aagccgacga tatccggctg tatgccgagg aaaatcactt cgccaatatc
    38341 ttctttcaac tggtcgataa gatagttcag aggcatgtta tgggtagtca tcataaacat
    38401 ctcggcgata tcatccgggt cgatgatgcg gatctcgccg gggtttagcc ccatatccgt
    38461 ggcgtcgaca atcagtaacc gcgtagggcg cagttcacgg atggcgacga tgtcgttttc
    38521 cggtgcacta ccgccgtcaa tcaccaccca gttacctttc ggcgcggcgg cgcacttttc
    38581 cgccagcagc ggacctgcgc catcatcgcc catcatgcta ttgccaacac agagtaaaac
    38641 gtcagtcacg caatctcctc accatcaaat agatggcgtt ttcctgatga atatcatgca
    38701 gcatacttaa cagcattgta ctccattcct gctgctgcga agtttgcgct gcccgggctt
    38761 tatcgaatgc gttggccagc atcggcacat ggttaatgtc gatgacgatc tcaccgtact
    38821 tcggtacgcc ttccattttg cggcgggctt cactgcccgc ctccagagtg gcaatccatg
    38881 ccagatattc atcccacggg caggtgagcg ccgcttccag gcaatcgata accccaaggt
    38941 ggtgaccaat cgccaggctg taatagacca cctgctgcgc ctcggcgggc gtggcatcgt
    39001 tctcatcaat aaatttacgg ctcagttgac tgaacaccac cttttcactc atcggatacg
    39061 cacctcttct acaacatgat tcagatggct gacaatctcg ttcagacgcg gatcgttttc
    39121 cgcttccagc cagcgtgcaa cctgttcttc gccctgccct aactgtgtca ggaagtcatc
    39181 ggcaatctga cgaccgtaac gataacccgc caggcggcgt gcttcgcgat ccactttcac
    39241 gcgcagcggc tgcaccatat cgccatgcag gatctccgcc ggttgttcat ccagttcacc
    39301 cggcccacgg gcgtgaattt tctgctccag caggccgagc gccattgcaa agccgtacag
    39361 cgtggcggca ggcgttggcg ggcagccagg gatataaaca tccacaggga caattttatc
    39421 cgtaccgccc cacacgcagt agagatcgtg gaagatcccg ccgctgttac cgcaggcacc
    39481 gtaggagata caaattttcg ggtccggcgc ggactgccac gcacgcagcg caggggatcg
    39541 cattgcacgg gtgaccgcgc cggtaaacag taaaatatcc gcatgacgcg gtgaaggaac
    39601 gactttaatg ccgaagcgtt ctgcatcaaa cagcggcgat aaagtggcga aaatttcgat
    39661 ttcgcagccg ttgcagccgc cgcagtccac gcggtaaaca taggcagaac gtttgatttt
    39721 tttcagtaac gacgccttca tgctggcgat ggattcatcc accgtcatcg ggaccggaat
    39781 gccgttggcg tcacgggggc ctaataaatt gctcatcaga tggcctcttt catatggcga
    39841 gtcagttcaa tacggtcgga cggcaccagg catttctggc gcttacattc cgggcaagtc
    39901 tcaaagcttt cgcggtggtt ttccgcgcgg ctgtcgccgt tgtgcttaag cagcgcaatg
    39961 gcgtagtcga tctctttctg gacggcgaaa ggacgattgc agacgcgaca gttgcacagc
    40021 gcgaagcgag actgttgcag gaagtcttct ttcttccaca ccgccagttc gtactcttgc
    40081 gacagcttaa tcgccgccgt cgggcaaact tcttcgcagc gtccacagaa gatgcagcgc
    40141 ccgagattaa actcccaggc cagctctcct gtggcgaggt cagtttcaac cgttaaggcg
    40201 tttgacgggc aggcattgac gcaggccgcg cagccgatgc actgctgcgg gttctgctct
    40261 ggcttaccac ggaagttttt atcaaccgca atcggctcca gcggataaga cgaggtcgcc
    40321 gtaccggttt tgataacttt tttgataaag gtaaacatgg cgattcctta tttcagcggc
    40381 gagtttttac gctcaatgct gtagcgctcg agttctttgt acggcaccac tttgctcttc
    40441 ttcttacgta catcgaccac agtcatgcgg tcggtacagg agtagcaagg gtccaggcta
    40501 ccgataatca gcggcgcatc ggaaacagtg ttgccacgca gcatgtagcg cagggtcggc
    40561 cagttcgcgt aggtcgcggc acggcagcgc cagcggtaca gcttctggtt gtcgccagtc
    40621 atgctccagt ggatatcatc accgcgcggc gcttcggcaa agcccagcgc gaaacggtgc
    40681 ggaatgtagg taaagccttc caccatcagc gggccacccg gcaggttatc cagaccgtag
    40741 tcgatcatgt tcagcgcggt atagacttcg ttgatacgca ctttcagacg cgaaataacg
    40801 tcgcagccct gctcgctgtg gacttccatt ggcagcaggc cataaccaac aaacgggtga
    40861 tcagcgcggg tatcgcgagc gtgtccgctg gcgcggacca tcgggccaac gttactgaaa
    40921 tcgcgggcaa tttccgggtc cagacgacca atgccgacgg tgcgctgttc catgttcggt
    40981 gtgctcagca gcacatccac cagctcctgc acttcacgac gcatctgttg cgccagctgg
    41041 cgggtctgga tcatgtcatc tttcaacaga tcgcgacgaa tcccgccgat caggttcagg
    41101 ccgtaggttt tacgcgcacc ggtgaggatc tcggccattt tcatggaggt ttcacgcacg
    41161 cggaagaatt gcataaagcc ggagtcgaag ccggtaaagt ggcaggcgag gccgaggttg
    41221 agcagatgcg agtgcaggcg ttccacctcc agcagaatgg cgcggatcat ctgcgcacgt
    41281 tctggcacct gaatacccat cgcgttttcc accgacgtgg tgtaggcggt gctgtgggcg
    41341 aagccgcaga tcccgcacac acggtcagag aggaaggtca cttcgttata acccatgcgg
    41401 gtttccgcca gtttttccat accgcgatgg acatagaaca agcggtagtc tgcgtcgata
    41461 atgttttcgc catcgacgaa cagacggaag tggcccggtt cgtcagaagt gacgtgcagc
    41521 ggaccaatcg gcacgacgtt gtttttcttg tcgcccagtt cgttgatgaa ctcgtaggtt
    41581 tcagcatcgg tggtcggtgc cggacgctga cgataatcca tgctgtcttt acgcagcgga
    41641 taaagttcat ccggccagtc atccggcagc accagacggc gttcatccgg cagaccaacc
    41701 ggaatcaaac cgtacatatc gcgcacttcg cgctcgcccc acaccgccgc cggaacgcgc
    41761 ggcgtcacgg acggatactc cggtttgttg gcatcaactt cgacgcgaac cgttacccag
    41821 cacttagtcc ccttctccat cgacagcacg taataaacgg cgtaatgacc attcagtttg
    41881 cgttcgtcgt taccaaaaag caccgacagc cagcccccct gcttgtagta gagaaactcc
    41941 accacttccg gcagatagtt cacctttacg gtgacagtca gctgctcttt ggtctgccag
    42001 gcgtggtcca gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct
    42061 aatttttctt cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg
    42121 ctaaaaatgc aaagccaaac ccagcccagg taatgcgcga agtaatatca agacgcagac
    42181 gggccatgct gttttcgaac agcgcgataa ccaggacgcc gaccaccagt tttacgatcg
    42241 caatcaccag cgccagcagc agtccaccgg cggtgaaggt ttccatttgt ccccacggaa
    42301 taaacacccc gacgaacatc tgcaacacca ccagctgttt caggctgata ccccatttca
    42361 tgacgccaaa gccgctgccg ctatactcag agagcggacc ttcctgcaac tcctgctcgg
    42421 cttccgccag gtcgaacggc agtttgccca tttcgataaa agtggcgaac gcacaggcgc
    42481 agagcgccag caccagcggg atgctttggc tcagcggcca gtgataaacg gtgtcggtaa
    42541 tgttgctgat gttggtggaa ccggcaacct gtgcggcaac ccacagacca agcagcaaca
    42601 tcggttcgac cagcacgcca agcatcgctt cacggctcgc accgatagcg gtaaacgggc
    42661 taccggtatc cagaccggaa atggcaaaga agaaacgcgc gatggcgaag agatacagta
    42721 aggtgatcaa atcgcccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag
    42781 tagcgatagt cagcatgacg cccaccatca catacggcgt caggcggaac acccagccag
    42841 aggcatccgg gccaacgctc tggcggccca gcagtttgat aatgtcgcga tactcctgca
    42901 acacgcccgg cccgcgacgg ttatgcagac gggcgcgcgc cacgcgggta ataccggaga
    42961 gcagcggcgc aacggcaaat aacaccagcg cctgaattaa cggatataaa acactcattc
    43021 tcaggctcct cgtgaaacaa taatcaccac cagtaccgcc agttcaacca gcgccatccg
    43081 gcggaacagc aacgcactac cttcgcactg ccagcccggc accagcgaca ccggattcag
    43141 ccatttgcgc agtttcagca ctggcgcaaa cgcctgttta accggcatgg caaaaccgtg
    43201 agcggtaatc accattgatt tttcatggtc gtagccgcac acccaggccg caccgcggga
    43261 acgtgacggc aggcggtcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca
    43321 ggcaatcagc agcaatgtga tcattggttg agaaacggtg gtgttagcag gctccagagg
    43381 cagaggtacg gcagcagaga gcatcggcag cagccacggc gcagcaacac cgccaattac
    43441 gcagcaaatc gccagtgcca ctacgcttac gctcatcagg agcggcgcac aggtggcgtt
    43501 ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca
    43561 catcaccgcc agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg
    43621 ggcaacaaac gcgccactat tgctcagttt gaaaaaggat tgatagataa cccattcccc
    43681 ggcaaacccg ttcagcggcg gcagcgcagc cattgccatc aacccgacta acatggcgat
    43741 ggagataacc ggcattttct tgccaatacc accgagtttt tcgatatcgc gatgaccggt
    43801 acggaaccag acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag
    43861 cagatggtac agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat
    43921 acccgttacg ccagcgccca gccccagcag gataatgccg atattttcca gggtgtggta
    43981 agccagcagg cgctggatgt tgtgctccat cagcgcatac agaccaccga caaacgcggt
    44041 gatcatgccg agcaccagca gcacgatccc ccaccacagc ggtgcattac cgcccagcag
    44101 tgacaggatt aaaatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc
    44161 agctggcgca gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc
    44221 ggcgagcagg ccaaagccaa tcacaccgag cagccagata tcggaaccga gcggcagctg
    44281 ttgcatacgc atatccagca ggcgcagatc cagcgtgcca taacgctgcc acagcagcca
    44341 gcaagcaatc gccagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc
    44401 tttgctgttg ctggtgagga acaccgcaca caaggccatg acttcggcca ttaccacgaa
    44461 catgccgagg ttgctggcga tgacggcgca gacggcagca gccatcaaca tattgatctg
    44521 caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttgtaga ggctgacaaa
    44581 cagaccgcac agaccgagcg taatcagcca aatcgcgtta agcggggaga tttgcacatc
    44641 gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagca cagtgaagcc
    44701 cgcggctgcc gtatacaggc taccaaccgc gccgccaatt ccggctatcc agccacttag
    44761 cgtcttttga aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc acaccacgcc
    44821 gctattgatc agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag
    44881 gtgagatcgc caaagtccgt attaaaagtc agctcacgct tacgtttgct gacgcgggcg
    44941 atatcggtgt tatccaccag atgcagggct ttggtcgggc acatccgcac gcaggccgga
    45001 ccttgttcat caaagctaca aaggtcacat ttgacggcga tcgcgcgaat acccggcacc
    45061 cagtcaagca gcgtactgat acgcgccgga gcaggcggtg ccggtggcgc tttcggggta
    45121 ttggcatttg caggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg
    45181 caggcgatgc cgcacagctt gcagcttacg cacaggcttt cattcaactg cacggcccca
    45241 tcaacgcggg tgatgacgtt aaccgggcag accaccgcgc agggtgcatc ttcacagtgg
    45301 tgacagagct gcggcgcaga ttctttttca ttcagcatca ctctcaggcg cggcattgat
    45361 tgcaggccgt gctggcgatg cgtctctgaa caggctgcct cacaagtgtg gcagccgata
    45421 cagagcgtgg agtcagcaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat
    45481 ttttgacgaa aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc
    45541 aaacaggcgt aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc
    45601 ctttaccagt tggcttaaat tcaccggcac attgttttca atggcggtgt ataagctgtc
    45661 gtaaacgcca gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt
    45721 gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc
    45781 ttcgccctct tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag
    45841 ggtgacgtaa atacgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca
    45901 gctcatggcg tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca
    45961 gtagatgtgc cactggtcct gtaggcgacg atgccgctgt gcgatgtaat cggctttctc
    46021 gcttatttcc caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc
    46081 attttctgtg ccaattttta atttattgtt attaaaacga tttttaagct aaagatgaat
    46141 ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat
    46201 caacactggc acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga
    46261 aataaccctc tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc
    46321 aaaacgcgta actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc
    46381 tcttgccttt tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca
    46441 cctcgaagaa caagaggtcg aatgctggtg tgaaacatgc caacagtatg tgacgctatt
    46501 gacccagcgc gtccgccgct gtccacagtg tcatggtgac atgctacaga ttgtggcaga
    46561 cgacggttta cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg
    46621 cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt
    46681 tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc
    46741 gcctgaattt acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc
    46801 gggcactcac gcaccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct
    46861 ggacaaaaac aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact
    46921 ggtgctcaac ctggtttcca gcccaggttc cggtaaaacc accctgctga cggaaaccct
    46981 aatgcgcctg aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa
    47041 cgatgccgca cgcattcgcg ctaccggcac accagcgatt caggtgaaca caggtaaagg
    47101 ctgccatctt gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa
    47161 cggtattctg tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg
    47221 tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata
    47281 tccgcatatg tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta
    47341 tctcaacttt gacgttgaga agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga
    47401 aatcatcctt atttccgcca ccagcggtga agggatggac cagtggctga actggctgga
    47461 gacacagcga tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggca
    47521 aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat
    47581 gaaaacggtc agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc
    47641 gtaattactg aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt
    47701 gagccggatg tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat
    47761 atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc
    47821 tctcttacac cgccgaacgc cctctgcgga ttatggaagt gtgcggtggt catacccacg
    47881 ctatttttaa attcggcctc gaccagttac tgcctgaaaa cgttgagttt atccacggtc
    47941 cgggttgccc ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca
    48001 gccatccgga agtcatcttc tgtacctttg gcgatgccat gcgcgtacca gggaaacagg
    48061 gatcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg
    48121 atgcgttgaa actggcgcag gagaatccaa cccgcaaagt ggtgttcttc ggcttaggtt
    48181 ttgaaaccac catgccgacc accgccatca ctctgcaaca ggcgaaagcg cgtgatgtgc
    48241 agaattttta cttcttctgc cagcacatta cgcttatccc gacgctgcgc agtttgctgg
    48301 aacagccgga taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg
    48361 gcactgatgc ctataatttt atcgccagcg attttcatcg tccgctggtg gtcgctggtt
    48421 tcgaacccct tgatctactg caaggcgtgg tgatgctggt ggagcagaaa atagcggccc
    48481 acagcaaggt agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc
    48541 aacaggcgat tgccgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg
    48601 tgattgaatc ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac
    48661 atttccgccc ggcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat
    48721 taacgggcaa atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa
    48781 ccgcatttgg tgcactgatg gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc
    48841 gtcagcagga gagtgaagcg tgaataatat ccaactcgcc cacggcagcg gtggccaggc
    48901 gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga
    48961 gcaggaagac caggcacgtc ttgatctggc gcagctggta gcagaaggcg atcgtctggc
    49021 gttctccacc gacagttacg tcattgaccc gctgttcttt cctggcggta atatcggcaa
    49081 gctggcgatt tgcggcaccg cgaatgacgt tgcggtcagt ggcgctattc cgtgctatct
    49141 ctcctgtggc tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac
    49201 cagcatggca gaaaccgccc gcacggcagg cattgccatc gttactggcg atactaaagt
    49261 ggtgcagcgc ggcgcggcag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc
    49321 ggcgaatatt cactggggcg cacagacgct aaccgcaggc gatgtattgc tggttagcgg
    49381 tacactcggc gaccacgggg cgactatcct taacctgcgt gaacaactgg ggctggatgg
    49441 tgaactggtc agcgactgcg cggtgctgac gccgcttatt cagacgctgc gtgacattcc
    49501 cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgta aacgcggtgg ttcatgagtt
    49561 cgcggcagcc tgcggttgtg gtattgaaat ttctgaatca gcactgccgg ttaaacctgc
    49621 tgtgcgcggc gtttgcgaac tgctgggact ggacgccctt aactttgcca acgaaggcaa
    49681 actagtaata gctgttgaac gcaacgcagc agagcaagtg ctggcagcgt tacattccca
    49741 tccactgggg aaagacgcgg cgctaattgg tgaagtggtg gaacgtaaag gtgttcgtct
    49801 tgccggtctg tatggtgtga aacgaaccct cgatttacca cacgccgaac cgcttccgcg
    49861 tatatgctaa taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg
    49921 cggtgctttc ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag
    49981 ggttgttcga catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg
    50041 ctctttcgca actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc
    50101 aagcgcagac tcaacgtgcg tcttattacg catcgcgtga aaaagacacc cccattaaat
    50161 atgaagacga aactgttctg gcacacggtc cggtacgccg cattttgtcg cgccctgata
    50221 cgctgcattg cagttacgaa gaattttgtg aaacctggcc gcagctggtc gctggtgggc
    50281 tataccccta atttggtcac tattgcctga tgccactggc ggcggaaggg catatttttg
    50341 gtggctgtga atttattcgt tatgacgatc gtccctggag cgaaaaagag ttcaatcgtc
    50401 tgcaaacatt tacgcagatc gtttctgtcg tcaccgaaca aatccagagc cgcgtcgtca
    50461 acaatgtcga ctatgagttg ttatgccggg agcgcgataa cttccgcatc ctggttgcca
    50521 ttaccaacgc ggtgctttcc cgcctggata tggacgaact ggtcagcgaa gtcgccaaag
    50581 aaatccatta ctatttcgac attgacgata tcagtatcgt cttacgcagc caccgtaaaa
    50641 acaaactcaa catctactcc actcactatc ttgataaaca acatcccgcc cacgaacaga
    50701 gcgaagtcga tgaagccgga accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc
    50761 tgatcaatct ccacgagcgg gacgatttag ccccctatga acgcatgttg ttcgacacct
    50821 ggggcaacca gattcaaacc ttgtgcctgt taccgctgat gtctggcgac accatgctgg
    50881 gcgtgctgaa actggcgcaa tgcgaagaga aagtgtttac cactaccaat ctgaatttgc
    50941 tgcgccagat tgccgaacgt gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa
    51001 tccatcgtct gaaagaacgg ctggttgatg aaaacctcgc cctgaccgag cagctcaaca
    51061 atgttgatag tgaatttggc gagattattg gccgcagcga agccatgtac agcgtgctta
    51121 aacaagttga aatggtggcg caaagtgaca gtaccgtgct gatcctcggt gaaactggca
    51181 cgggtaaaga gctgattgcc cgcgcgatcc ataatctcag tgggcgtaat aatcgccgca
    51241 tggtcaaaat gaactgcgcg gcgatgcctg ccggattgct ggaaagcgat ctgtttggtc
    51301 atgagcgtgg ggcttttacc ggtgccagcg cccagcgcat tggtcgtttt gaactggcgg
    51361 ataaaagctc cctgttcctc gacgaagtgg gcgatatgcc actggagtta cagccgaagt
    51421 tgctgcgtgt attgcaggaa caggagtttg aacgtctcgg cagcaacaaa atcattcaga
    51481 cggacgtacg tttaattgcc gcgactaacc gcgatctgaa aaaaatggtc gccgaccgag
    51541 agttccgtag cgatctctat taccgcctga acgtattccc gattcacctg ccgccactac
    51601 gcgagcgtcc ggaagatatt ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc
    51661 gtctggggcg caatatcgac agcattccag ccgagacgct gcgcaccttg agcaacatgg
    51721 agtggccggg taacgtacgc gaactggaaa acgtcattga gcgcgcagta ttgctaacac
    51781 gcggcaacgt gctgcagctg tcattgccag atattgcttt accggagcct gaaacgccgc
    51841 ctgccgcaac ggttgtcgct caggagggcg aagatgaata tcagttgatt gttcgcgtgc
    51901 tgaaagaaac caacggcgtg gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga
    51961 aacgcacgac cctgctgtca cggatgaagc ggctgggaat tgataaatcg gcattgattt
    52021 aactgcaaat tgccggacag atctgcctgt ccggcatact attcacgagg ttttttcgga
    52081 cgatattttt ccggcagttc tggcaccgga cgcttgtcat cgatgagatg acgcacggtt
    52141 aagatcggat gacgccacag cattctcggc ccggcccaac gcataatctg tttcatctct
    52201 tcacgctttg caggctggta acagtgcacc ggacactgct tacaggctgg tttctcttcg
    52261 ccgaacacac atttatccag ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc
    52321 ggctccgctg acgcctgcgg gcatttcgct tgataaagat cgatcatttt tttaatcgtc
    52381 agtttttcac gagagatacg cttgccggac atgctgcctc catactctta aggtgtattt
    52441 atattacaac ttaatttgaa aggggactat ggctctgaag gaggaggttt attcaaccga
    52501 cgcaattttg cgtacttgct tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc
    52561 cggaaccagc atcaagaact cgccgttcgc ttcttcccct gaaatgatta actccggtat
    52621 catgtgcgcc ttatgtgatt acaacgaaaa taaaaaccat cacaccccat ttaatatcag
    52681 ggaaccggac ataaccccat gagtgcaata gaaaatttcg acgcccatac gcccatgatg
    52741 cagcagtatc tcaggctgaa agcccagcat cccgagatcc tgctgtttta ccggatgggt
    52801 gatttttatg aactgtttta tgacgacgca aaacgcgcgt cgcaactgct ggatatttca
    52861 ctgaccaaac gcggtgcttc ggcgggagag ccgatcccga tggcggggat tccctaccat
    52921 gcggtggaaa actatctcgc caaactggtg aatcagggag agtccgttgc catctgcgaa
    52981 caaattggcg atccggcgac cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt
    53041 acgccaggca ccatcagcga tgaagccctg ttacaggagc gtcaggacaa cctgctggcg
    53101 gctatctggc aggacagcaa aggtttcggc tacgcgacgc tggatatcag ctccgggcgt
    53161 tttcgcctga gcgaaccggc cgaccgcgaa acgatggcgg cagagctgca acgcactaat
    53221 ccggcggaac tgctgtatgc agaagatttc gccgagatgt cgctgattga aggccgtcgc
    53281 ggcctgcgcc gtcgcccgct gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat
    53341 ctgcaatttg gcacccgcga tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt
    53401 tgtgctgccg gttgtctgtt gcagtatgcg aaagataccc aacgcacgac cctgccgcat
    53461 attcgttcta tcaccatgga acgtgagcag gacagcatca ttatggatgc cgcgacacgt
    53521 cgtaacctgg aaattactca gaacctggcg ggcggtgcgg aaaatacgct ggcttctgtg
    53581 ctcgactgca ccgtcacgcc gatgggtagt cgtatgctga aacgctggct gcatatgcca
    53641 gtgcgcgata cccgcgtgtt gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc
    53701 accgccgggc tacagccggt actgcgtcag gtcggcgacc ttgaacgtat tctggcacgt
    53761 ctggctttac gaactgctcg cccacgcgat ctggcccgta tgcgccacgc tttccagcaa
    53821 ctgccggagc tgcgtgcaca gttagaaact gtcgatagtg caccggtaca ggcgctacgt
    53881 gagaagatgg gcgagtttgc cgagctgcgc gatctgctgg agcgagcaat catcgacaca
    53941 ccgccggtgc tggtacgcga cggtggtgtt atcgcatcgg gctataacga agagctggat
    54001 gagtggcgcg cgctggctga cggcgcgacc gattatctgg agcgtctgga agtccgcgag
    54061 cgtgaacgta ccggcctgga cacgctgaaa gttggcttta atgcggtgca cggctactac
    54121 attcaaatca gccgtgggca aagccatctg gcacccatca actacatgcg tcgccagacg
    54181 ctgaaaaacg ccgagcgcta catcattcca gagctaaaag agtacgaaga taaagttctc
    54241 acctcaaaag gcaaagcact ggcactggaa aaacagcttt atgaagagct gttcgacctg
    54301 ctgttgccgc atctggaagc gttgcaacag agcgcgagcg cgctggcgga actcgacgtg
    54361 ctggttaacc tggcggaacg ggcctatacc ctgaactaca cctgcccgac cttcattgat
    54421 aaaccgggca ttcgcattac cgaaggtcgc catccggtag ttgaacaagt actgaatgag
    54481 ccatttatcg ctaacccgct gaatctgtcg ccgcagcgcc gtatgttgat tattaccggc
    54541 ccgaacatgg gcggtaaaag tacctatatg cgccagaccg cactgattgc gctgatggcc
    54601 tacattggca gctatgtacc ggcacaaaaa gtcgagattg gacctatcga tcgcatcttt
    54661 acccgcgtag gcgcggcaga tgacctggct tccgggcgct caacctttat ggtggagatg
    54721 actgaaaccg ccaatatttt acataacgcc accgaataca gtctggtgtt aatggatgag
    54781 atcgggcgtg gaacgtccac ctacgatggt ctgtcgctgg cgtgggcgtg cgcagaaaat
    54841 ctggcgaata agattaaggc attgacgctg tttgccaccc actatttcga gctgacccag
    54901 ctaccggaga aaatggaaga cgtcgccaac gtgcatctcg atgcgctgga gcacggagac
    54961 accattgcct ttatgcatag cgtgcaggat ggcgcggcga gcaaaagcta cggcctggcg
    55021 gttgcagctc tggcaggcgt gccaaaagag gtgattaagc gcgcacggca aaaactgcgt
    55081 gagctggaaa gcatttcgcc gaacgccgcc gctacgcaag tggatggtac gcaaatgtct
    55141 ttgctgtcag taccggaaga aacctcgcca gcggtcgaag ctctggaaaa cctggacccg
    55201 gattcactga ctccgcgtca ggcgctggaa tggatttatc gcttgaagag cctggtgtaa
    55261 taacaattcc cgatagtctt ttgctatcgg gaatattaac gacaactgac gaataaaata
    55321 aaaaccccct gtataatagg aaagcttatt ttacagggta aaaccatgcc atctacacgc
    55381 tatcaaaaaa ttaatgccca tcactatcgc catatatggg tcgttggtga tattcatggt
    55441 gaatatcaat tattacaatc ccgcttacat caactctctt tttgccctga aaccgactta
    55501 cttatttctg tcggcgataa tattgatcgt gggccggaga gtcttgacgt cctgcgcctg
    55561 ctaaaccagc cctggtttat ctccgttaaa ggcaaccacg aagcaatgga gctggatgcg
    55621 ttcgcgaccg gcgatggcaa tatgtggctt gccagcggtg gtgactggtt tttcgattta
    55681 aatgattcag agcaacaaga ggcaatagat ctgttgctga aattccatca ccttccacat
    55741 attattgaaa tcactaacga cataataaaa tatgtcatcg cacatgcaga ttatccgggt
    55801 gatgaatatc tctttggcaa agaaatagcg gagagcgaat tactctggcc tgtttatcgt
    55861 gtgcagaaat cgcttaatgg cgagttacaa caaataaacg gcgctgatta ttttatattt
    55921 ggacatatga tgtttgataa cattcagacg ttcgctaacc agatttatat tgataccgga
    55981 tcgccgaaaa gtgggcggct gtcattttat aaaataaggt agtctcatgc ttcttctgtg
    56041 aagcatgagt aaccagatgt tattgcaggc cattattcat ttttcgctac cagcaaagag
    56101 agatcctgct tcgccagcgc gcgactggca ctctccggca aaccgtcgtc tgtaataatc
    56161 tgatcaaact cgcttaatgg taacgccagc catgtcgcca cctgaccata tttcgtcgca
    56221 tcacagacca aaactcgctg gcggctggca ctagcaatcg cccgtttcac cgtgacttta
    56281 tcttccgctg gcgtagaaat cccccgcaca ctccatgacg atgcagaaat aaaagcctga
    56341 tcaatcatca ggctgcgcag catggtcgca gcggcttccc cgacacagga acggttttcc
    56401 cgacacactg caccgccagt gtgaataatt gtgcaattac tgttgtcgag caagtagttc
    56461 gcaataacga aatcgtttgt gaccacagtc agtgactcca tgtgaatcag atgctgtgct
    56521 atcgctaacg tggtcgttcc cgcatccaga tagatacaac ttcccggctg aacaagactt
    56581 gccgccagct tgccaatagc cgctttttgc gtcattgcta gcgcagtttt tacctgatga
    56641 gaaggttcat gcgccacgcg tcccggagac tggacgcctc cggacaccag cacaacggct
    56701 ccctgctgct ccagtttttg taaatcccga cgaatggtca tatgtgatac attcattctg
    56761 tccgttagtt cagcaatact gacaatgcct ttttcagcta ccatctcaag gatgatttgg
    56821 cgacgctcta cgggtatcaa cttttgctcc ttcctttgtc ctgctgacat tctacgctat
    56881 ttgcctgcga aacgtgcgcg gcgcaactaa cgcttagttc acataaaata acacacaatg
    56941 ttaatttatg tgaataggct caccatatcg ttatcttcca gcgcttatat tcacaatatc
    57001 aaacaaaata tcacttaaat taacaaggag agcagatgaa aacgggatct gagtttcatg
    57061 tcggtatcgt tggcttaggg tcaatgggaa tgggagcagc actgtcatgt gtccgcgcag
    57121 gtctttctac ctggggcgca gacctgaaca gcaatgcctg cgctacgttg aaagaggcag
    57181 gtgcttgcgg ggtttctgat aacgccgcga cgtttgccga aaaactggac gctctgctgg
    57241 tgctggtggt caatgcggcc caggttaaac aggtgctgtt tggtgaaaca ggcgttgcac
    57301 aacatctgaa acccggtacg gcagtaatgg tttcttccac tatcgctagt gctgatgcgc
    57361 aagaaattgc taccgctctg gctggattcg atctggaaat gctggatgcg ccagtttctg
    57421 gtgatgcagt aaaagccgct aacggtgaaa tgactgtcat ggcctccggt agcgatattg
    57481 cctttgaacg actggcaccc gtgctggaag ccgttgccgg aaaagtttat cgcataggtg
    57541 cagaaccggg actaggttcg accgtaaaaa ttattcacca gttgttagcg ggcgtacata
    57601 ttgctgccgg agccgaagcg atggcacttg cagcccgtgc ggggatcccg ctggatgtga
    57661 tgtatgacgt cgtgaccaat gccgccggaa attcctggat gttcgaaaac cggatgcgtc
    57721 atgtggtgga tggcgattac accccgcatt cagccgtcga tatttttgtt aaggatcttg
    57781 gtctggttgc cgatacagcc aaagccctgc acttcccgct gccattggcc tcaacagcat
    57841 tgaatatgtt caccagcgcc agtaacgcag gttacgggaa agaagacgat agcgcagtta
    57901 tcaagatttt ctctggcatc actctaccgg gagcgaaatc atgatcaaga ttggcgttat
    57961 cgccgatgat tttaccggcg cgacggatat cgccagtttt ctggtggaaa acggtctacc
    58021 aacggtacaa attaacggtg ttccaacagg taaaatgccg gaagcaatcg acgcactggt
    58081 gatcagcctg aaaacgcgct cctgtccggt ggttgaagcc acacagcaat cgctggcggc
    58141 tctgagctgg ttgcaacagc aaggttgcaa acagatctat ttcaaatact gctctacttt
    58201 cgacagtacg gcgaaaggta atatcggccc ggttaccgat gcattaatgg atgctctcga
    58261 cacgccgttt acggtcttct ctccggccct gtcggtcaac ggacgtacgg tttatcaggg
    58321 gtatttgttc gtaatgaatc aactgctggc cgaatccggg atgcgccatc acccggtaaa
    58381 tcccatgacc gacagctatc ttccccgtct ggttgaagcg caatccacag ggcgctgcgg
    58441 cgtcgtttcg gcacatgttt tcgaacaagg tgtggatgcc gttcgtcaag agctggctcg
    58501 cttacagcaa gagggctacc gctacgcggt gcttgatgcg ctgaccgaac accatctgga
    58561 aattcaggga gaagccttgc gcgatgcccc actggtaacg ggcggttctg gtctggcgat
    58621 tggcctggcc cggcagtggg cgcaagaaaa cggtaaccag gctcgcgaag cagggcatcc
    58681 gctcgctggg cgcggcgtag tgctctccgg ttcatgctct caaatgacca accgccaggt
    58741 ggcacattac cgtcaaattg caccagcccg tgaagttgat gtggcacgct gcctctcaac
    58801 cgaaactctg gccgcttatg cacacgaact ggcagagtgg gttctgggcc aggaaagtgt
    58861 acttgctcca ctggtttttg ccaccgccag cactgacgca ttggcagcaa ttcaacagca
    58921 atacggtgca caaaaagcca gtcaggcagt agaaactctg ttttctcaac tagcggcgcg
    58981 gttagcagcg gaaggcgtga cacgctttat tgtcgcaggc ggtgagacct ccggcgtagt
    59041 cacacagagc ctgggcataa aagggtttca tattggccca accatttccc ccggcgtgcc
    59101 gtgggtaaac gcactggata agcctgtctc actcgccctt aaatctggca acttcggtga
    59161 tgaagccttt ttttcacgag cccaaagaga gtttttatca tgagcgattt cgcaaaagta
    59221 gagcagtctt tgcgagagga gatgacgcgg attgccagtt cattctttca gcgcggctat
    59281 gcaaccggtt cggctggcaa tctgtcgctg cttttacctg acgggaattt actggcgaca
    59341 ccgacaggtt catgcctggg caatctcgat ccgcagcggc tttccaaagt cgccgcggat
    59401 ggcgaatggt taagtggtga caaaccctcg aaagaggtgc tctttcatct ggcgctgtat
    59461 cgcaacaatc cgcgctgtaa agcggtggtg catttgcaca gcacatggtc gacggtgctt
    59521 tcctgcctgc aagggctgga cagcagcaac gttattcgtc cgttcacacc atacgtggtg
    59581 atgcggatgg gaaatgtccc gctggtgccc tattaccgac cgggcgataa acgcatcgca
    59641 caggatctgg cggaactggc agcagacaat caggcttttt tactggcaaa tcatggccca
    59701 gtggtttgcg gtgaaagcct gcaagaagcc gccaacaata tggaagagct ggaggaaacg
    59761 gcaaagctga tttttattct cggtgaccgc ccgatccgtt atctgaccgc aggtgaaatt
    59821 gcggaattaa ggagttaatg caatgcctcg ttttgcagct aatttatcca tgatgttcac
    59881 cgaagtgcct tttattgaac gcttcgccgc agcgcgaaaa gccgggttcg atgctgtgga
    59941 atttctgttt ccctatgact actccaccct gcaaatccaa aagcaactgg agcaaaacca
    60001 tctgacactg gcgctgttca ataccgctcc cggagatatt aatgcggggg aatggggatt
    60061 atccgccctt cccggacgtg agcacgaagc acacgcagac atcgacctgg cactggaata
    60121 tgcattggcg ctcaattgtg aacaagtcca tgtgatggca ggcgtcgtgc ccgctggcga
    60181 agatgctgaa cggtaccggg cagtatttat cgataatctc cgctacgctg ccgaccgctt
    60241 tgcgccacac ggcaagcgaa tattagttga agcactcagc cccgacgtta agcctcatta
    60301 tctcttctcc agccagtatc aggcactggc tattgttgaa gaggttgcgc gagataacgt
    60361 gtttattcaa ctagatactt ttcatgcgca aaaagtagat ggcaacctga cgcatttaat
    60421 ccgcgactac gctgggaaat atgcgcacgt acaaattgcc ggactacctg atcgacatga
    60481 ccggacgatg gagaaatcaa ctatccgtgg ctgttccgcc tgttcgatga ggtgggatat
    60541 cagggctgga tcggttgtga atataaacct cgtggcctca ccgaagaagg gcttggctgg
    60601 tttgacgcct ggcgctaatt cgttaaatcc ctaattacaa cgtacccata catcccccca
    60661 tctacggatg gggtaggtta actataactt tcagacaggg tttcccatgt ccacaattac
    60721 attgttatgc attgcgttag ctggcgtaat catgctgttg ctgctggtca tcaaggcaaa
    60781 ggtacaacca ttcgttgctc tgctcctcgt cagcctgtta gtcgcacttg cggcgggtat
    60841 accggcgggc gaagtgggta aagtgatgat cgccgggatg ggcggtgtgc ttggttccgt
    60901 cactattatt attggtctgg gcgctatgct ggggcgtatg atcgaacact ctggtggtgc
    60961 agagtcactg gctaattatt tcagtcgcaa gttaggtgac aaacgaacta tcgctgcgct
    61021 gactctggca gcgttcttcc tcggtattcc cgtcttcttt gatgtcagct ttattattct
    61081 tgcgccaatc atttacggtt ttgccaaggt tgccaaaata tcgccactca aatttggcct
    61141 gcctgtcgct ggaatcatgc taactgttca tgtggcagta ccgccgcatc caggccctgt
    61201 cgccacagcg gggttactcc acgcagacat cggctggcta accatcatcg gtattgcgat
    61261 ttctattccc gtagggattg ttggctactt tgcagcgaaa ataatcaata agcgccaata
    61321 tgcgatgtca gtagaagtac tggaacagat gcaactggct ccggccagtg aggaaggcgc
    61381 gacaaaatta agcgataaaa taaatccacc gggcgtcgcg ctggtcacct cgctaattgt
    61441 tattcctatc gcgattatca tgacgggtac ggtttccgca acactgatgc cgccttcgca
    61501 tcccctgctt ggtacgctac agctgatcgg ctcaccaatg gtagctctaa tgattgcgct
    61561 ggtgctggca ttctggttat tggctttacg tcgcggctgg agcttacaac acaccagcga
    61621 cattatgggc tcagcccttc ccactgcggc ggtagtgatt ttggttactg gtgctggagg
    61681 ggtatttggc aaagtgctgg tggaatcggg cgtcggcaaa gcccttgcca atatgctaca
    61741 aatgattgac ctgcctctgt taccagccgc atttattatt tcactggctc tgcgtgcatc
    61801 gcaggggtca gcaaccgtag caatcctgac aaccggcggg ttactctcag aagcggtgat
    61861 gggattgaat ccgattcagt gcgtattggt gacgctggca gcctgctttg gtgggcttgg
    61921 cgcgtcacat attaatgact cagggttctg gattgtgacc aaatatctgg ggttgtcggt
    61981 agcagacggt ctgaaaacct ggacagtgtt aacgaccatt ctcggtttta ccggattctt
    62041 aattacctgg tgcgtatggg cggtaatttg accttaataa aaaggtccga tgggcatcgg
    62101 accttttatt gtgcacagaa aaggccagcc tcgcttgaga ctggcctttc tgacagatgc
    62161 ttacttactc gcggaacagc gcttcgatat tcagcccctg cgtttgcagg atttcacgca
    62221 aacggcgcag gccttcaacc tgaatctggc gaacacgttc acgggtgagg ccaatttcac
    62281 gacctacatc ttccagtgtt gccgcttcgt accccagcaa accgaatcga cgtgccagca
    62341 cttcacgctg tttggcgttc agctcgaaca gccatttgac gatgctctgc ttcatatcgt
    62401 catcttgcgt ggtatcttcc ggaccgttct ctttttcatc ggccaggatg tccagcaacg
    62461 ctttttcgga atcaccaccc agcggggtgt ctaccgaggt aatgcgctcg ttaagacgaa
    62521 gcatacggct gacgtcatca actggcttat ccagttgctc tgcgatctct tccgcacttg
    62581 gttcatggtc cagcttatgg gacaactcac gtgcggttcg caggtaaacg ttcagctcct
    62641 ttacgatgtg aatcggcaaa cgaatagtac gggtttggtt cataatcgcc cgttcaatcg
    62701 tctggcgaat ccaccaggtt gcgtatgttg agaagcggaa accacgttcc gggtcaaact
//
LOCUS       DAJTSL010000005        61444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTSL010000005 DAJTSL010000000
VERSION     DAJTSL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.005"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..363
                     /locus_tag="OO433_000278"
     CDS             <1..363
                     /locus_tag="OO433_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCR6048890.1"
                     /translation="GLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDD
                     TGEVQAVELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMV
                     EIVDGVDLMEAHRNALGL"
     gene            418..720
                     /locus_tag="OO433_000279"
     CDS             418..720
                     /locus_tag="OO433_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6048891.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            735..854
                     /locus_tag="OO433_000280"
     CDS             735..854
                     /locus_tag="OO433_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCR6048892.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRDKAIQRSGASHE"
     gene            847..3924
                     /locus_tag="OO433_000281"
     CDS             847..3924
                     /locus_tag="OO433_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6048893.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISANDGASATARITRETMRYNRQLSEQEAR
                     LRRVGEQQRKMHAARGAYARRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQGAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTATVTRTSNALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAATGTVLRMLASGPLAMLRVALYAVSGLLGALLSLIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAATPAELPPLHPLALPAKGGEAMVSRAATVPPV
                     QRIEAPMQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            3921..4406
                     /locus_tag="OO433_000282"
     CDS             3921..4406
                     /locus_tag="OO433_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6048894.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEVTGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     S"
     gene            4403..5503
                     /locus_tag="OO433_000283"
     CDS             4403..5503
                     /locus_tag="OO433_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HCR6048895.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKLVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPLDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            5594..5812
                     /locus_tag="OO433_000284"
     CDS             5594..5812
                     /locus_tag="OO433_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR6048896.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(6047..7732)
                     /locus_tag="OO433_000285"
     CDS             complement(6047..7732)
                     /locus_tag="OO433_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6048897.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRI
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8002..8379
                     /locus_tag="OO433_000286"
     CDS             8002..8379
                     /locus_tag="OO433_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6048898.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8409..8666)
                     /gene="grxA"
                     /locus_tag="OO433_000287"
     CDS             complement(8409..8666)
                     /gene="grxA"
                     /locus_tag="OO433_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6048899.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            8826..9113
                     /locus_tag="OO433_000288"
     CDS             8826..9113
                     /locus_tag="OO433_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6048900.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9097..9819
                     /gene="nfsA"
                     /locus_tag="OO433_000289"
     CDS             9097..9819
                     /gene="nfsA"
                     /locus_tag="OO433_000289"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6048901.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            9880..10782
                     /gene="rimK"
                     /locus_tag="OO433_000290"
     CDS             9880..10782
                     /gene="rimK"
                     /locus_tag="OO433_000290"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6048902.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERYATTEYCLKTGG"
     gene            10870..11346
                     /locus_tag="OO433_000291"
     CDS             10870..11346
                     /locus_tag="OO433_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6048903.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11697..12809
                     /gene="potF"
                     /locus_tag="OO433_000292"
     CDS             11697..12809
                     /gene="potF"
                     /locus_tag="OO433_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6048904.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            12904..14037
                     /gene="potG"
                     /locus_tag="OO433_000293"
     CDS             12904..14037
                     /gene="potG"
                     /locus_tag="OO433_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6048905.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14047..15000
                     /gene="potH"
                     /locus_tag="OO433_000294"
     CDS             14047..15000
                     /gene="potH"
                     /locus_tag="OO433_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6048906.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVAAAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            14997..15842
                     /gene="potI"
                     /locus_tag="OO433_000295"
     CDS             14997..15842
                     /gene="potI"
                     /locus_tag="OO433_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6048907.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            15902..16390
                     /locus_tag="OO433_000296"
     CDS             15902..16390
                     /locus_tag="OO433_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6048908.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16431..17588
                     /gene="rlmC"
                     /locus_tag="OO433_000297"
     CDS             16431..17588
                     /gene="rlmC"
                     /locus_tag="OO433_000297"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6048909.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQSEAAGAASDC"
     gene            complement(17732..18463)
                     /gene="artJ"
                     /locus_tag="OO433_000298"
     CDS             complement(17732..18463)
                     /gene="artJ"
                     /locus_tag="OO433_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6048910.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18754..19422)
                     /gene="artM"
                     /locus_tag="OO433_000299"
     CDS             complement(18754..19422)
                     /gene="artM"
                     /locus_tag="OO433_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6048911.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19422..20138)
                     /gene="artQ"
                     /locus_tag="OO433_000300"
     CDS             complement(19422..20138)
                     /gene="artQ"
                     /locus_tag="OO433_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6048912.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESEKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20145..20876)
                     /gene="artJ"
                     /locus_tag="OO433_000301"
     CDS             complement(20145..20876)
                     /gene="artJ"
                     /locus_tag="OO433_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6048913.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYNSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(20894..21622)
                     /gene="artP"
                     /locus_tag="OO433_000302"
     CDS             complement(20894..21622)
                     /gene="artP"
                     /locus_tag="OO433_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6048914.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(21840..22355)
                     /locus_tag="OO433_000303"
     CDS             complement(21840..22355)
                     /locus_tag="OO433_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6048915.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPGNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            22481..22804
                     /locus_tag="OO433_000304"
     CDS             22481..22804
                     /locus_tag="OO433_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6048916.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            22801..23631
                     /gene="amiD"
                     /locus_tag="OO433_000305"
     CDS             22801..23631
                     /gene="amiD"
                     /locus_tag="OO433_000305"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6048917.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(23628..24638)
                     /locus_tag="OO433_000306"
     CDS             complement(23628..24638)
                     /locus_tag="OO433_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6048918.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMFSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLCSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     FRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGKL
                     PR"
     gene            complement(24737..26167)
                     /locus_tag="OO433_000307"
     CDS             complement(24737..26167)
                     /locus_tag="OO433_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6048919.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(26178..27179)
                     /gene="ltaE"
                     /locus_tag="OO433_000308"
     CDS             complement(26178..27179)
                     /gene="ltaE"
                     /locus_tag="OO433_000308"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6048920.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKVRNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(27216..28934)
                     /gene="poxB"
                     /locus_tag="OO433_000309"
     CDS             complement(27216..28934)
                     /gene="poxB"
                     /locus_tag="OO433_000309"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6048921.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(29067..30035)
                     /gene="hcr"
                     /locus_tag="OO433_000310"
     CDS             complement(29067..30035)
                     /gene="hcr"
                     /locus_tag="OO433_000310"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6048922.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVHTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAKAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(30047..31699)
                     /gene="hcp"
                     /locus_tag="OO433_000311"
                     /gene_synonym="priS"
     CDS             complement(30047..31699)
                     /gene="hcp"
                     /locus_tag="OO433_000311"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6048923.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVSAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATIVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLADKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(31843..32742)
                     /gene="lysO"
                     /locus_tag="OO433_000312"
     CDS             complement(31843..32742)
                     /gene="lysO"
                     /locus_tag="OO433_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6048924.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(33236..33931)
                     /gene="aqpZ"
                     /locus_tag="OO433_000313"
     CDS             complement(33236..33931)
                     /gene="aqpZ"
                     /locus_tag="OO433_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR6048925.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATAKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            34357..36015
                     /locus_tag="OO433_000314"
     CDS             34357..36015
                     /locus_tag="OO433_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6048926.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAIEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(36012..36968)
                     /locus_tag="OO433_000315"
     CDS             complement(36012..36968)
                     /locus_tag="OO433_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HCR6048927.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLKLTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            37119..38234
                     /gene="macA"
                     /locus_tag="OO433_000316"
     CDS             37119..38234
                     /gene="macA"
                     /locus_tag="OO433_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6048928.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAEIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            38231..40177
                     /gene="macB"
                     /locus_tag="OO433_000317"
     CDS             38231..40177
                     /gene="macB"
                     /locus_tag="OO433_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6048929.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHMVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALVRE"
     gene            complement(40250..40474)
                     /gene="cspD"
                     /locus_tag="OO433_000318"
     CDS             complement(40250..40474)
                     /gene="cspD"
                     /locus_tag="OO433_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6048930.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            40797..41117
                     /gene="clpS"
                     /locus_tag="OO433_000319"
     CDS             40797..41117
                     /gene="clpS"
                     /locus_tag="OO433_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6048931.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            41148..43424
                     /gene="clpA"
                     /locus_tag="OO433_000320"
     CDS             41148..43424
                     /gene="clpA"
                     /locus_tag="OO433_000320"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6048932.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(43768..43855)
                     /locus_tag="OO433_000321"
     tRNA            complement(43768..43855)
                     /locus_tag="OO433_000321"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43819..43821),aa:Ser,seq:gga)
     gene            complement(44109..44327)
                     /gene="infA"
                     /locus_tag="OO433_000322"
     CDS             complement(44109..44327)
                     /gene="infA"
                     /locus_tag="OO433_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6048933.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(44612..45316)
                     /gene="aat"
                     /locus_tag="OO433_000323"
     CDS             complement(44612..45316)
                     /gene="aat"
                     /locus_tag="OO433_000323"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6048934.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(45358..47079)
                     /gene="cydC"
                     /locus_tag="OO433_000324"
     CDS             complement(45358..47079)
                     /gene="cydC"
                     /locus_tag="OO433_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6048935.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMMVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(47080..48846)
                     /gene="cydD"
                     /locus_tag="OO433_000325"
     CDS             complement(47080..48846)
                     /gene="cydD"
                     /locus_tag="OO433_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6048936.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQCGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(48969..49934)
                     /gene="trxB"
                     /locus_tag="OO433_000326"
     CDS             complement(48969..49934)
                     /gene="trxB"
                     /locus_tag="OO433_000326"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6048937.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            50479..50973
                     /gene="lrp"
                     /locus_tag="OO433_000327"
     CDS             50479..50973
                     /gene="lrp"
                     /locus_tag="OO433_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6048938.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            51108..55058
                     /gene="ftsK"
                     /locus_tag="OO433_000328"
     CDS             51108..55058
                     /gene="ftsK"
                     /locus_tag="OO433_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6048939.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVTSVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSAAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQLVAPQQQYQQPQQQVAPQPQYQQPQQPV
                     APQQQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVE
                     ARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEV
                     IPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMP
                     HLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVV
                     TDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGD
                     SMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRP
                     SVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRV
                     HGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQF
                     VTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            55213..55824
                     /gene="lolA"
                     /locus_tag="OO433_000329"
     CDS             55213..55824
                     /gene="lolA"
                     /locus_tag="OO433_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6048940.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            55835..57178
                     /gene="rarA"
                     /locus_tag="OO433_000330"
                     /gene_synonym="mgsA"
     CDS             55835..57178
                     /gene="rarA"
                     /locus_tag="OO433_000330"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6048941.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            57269..58561
                     /gene="serS"
                     /locus_tag="OO433_000331"
     CDS             57269..58561
                     /gene="serS"
                     /locus_tag="OO433_000331"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6048942.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            58800..61244
                     /gene="dmsA"
                     /locus_tag="OO433_000332"
                     /pseudo
     CDS             58800..61244
                     /gene="dmsA"
                     /locus_tag="OO433_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            61255..>61444
                     /locus_tag="OO433_000333"
     CDS             61255..>61444
                     /locus_tag="OO433_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6048943.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYL"
BASE COUNT        15064 a        15425 c        16577 g        14378 t
ORIGIN      
        1 ggactggatg acagtgcact ggatacagaa ttttccattg gtggtactga actgctgctg
       61 tttaagcaga tgggcaaagc cacggtggat ggcatccagc tgcgctttac cggctctatt
      121 cagcgtgatg ataccgggga agtgcaggcc gtggagcttg tggtgcgtgg acgtcacaaa
      181 gaagtagatt ccggcgagtg gaagacgggc gaaagcaaca ctaccaaagt gaccagtacc
      241 aacagctacg cgaagctgac catcaatggt gaggtgctct atgaagtgga cctgatcaac
      301 atggtggaaa ttgtggacgg tgtggacctg atggaagcgc accgcaacgc cctcggcctc
      361 tgatgtatct gaacggcgcg gaatgccgcg ccagaaccta atttacagga caacaaaatg
      421 agcgataagc agactgaaaa gaccattcaa ctggatactc ccatcatgcg cggtaaaaca
      481 gaaattaccg aaattgtgtt gcgtaaaccg cagtccggtg cgctgcgcgg cacacgcctg
      541 caggccatta tggatatgga tgtgaacgcg atgatgaccg tgatcccccg catctccagt
      601 ccggcactga ctgcacagga aattgcagag atggacccgg cagatctcac tgccatgtcg
      661 gttgaggttg tcactttttt gttgaagaag tcggtgcttg ccggtttacc gacagcctga
      721 cggttgacga tctggtggct gatatcgcca ccatctttca ctggccgcca tccgttactg
      781 acgttatgcc gctgaccgaa gtgctggaat ggcgggataa agcgattcag agaagcgggg
      841 ccagccatga gtgacaataa cctgcgtctg caggtgattc ttaatgcggt tgacaaactc
      901 acccgcccat ttcgatccgc gcaggccagt tcaagagaac tggctgctgc tgtcaaaaaa
      961 tcccgcgatg caataaagca gcttgatcag gccgggagca gtctggacag cttccgaaag
     1021 ctgcaggcag aaaatcagaa attaggcgac aggctgaact atgcccgcca gcgtgcaaat
     1081 ttgctcagtc aggaactggg agcgatgggg ccgccttcgc aacgtcaggt tgttgctctg
     1141 ggccgtcaac ggctggctgt tcagcgccta gaagaacgcc agaaaaagct gcagcagcag
     1201 acggcgcttg tgcgtgcaga actttatcgt gctggtattt cagctaatga tggcgccagt
     1261 gcgacggccc gcattacccg tgaaacgatg cgttataaca ggcagctttc tgagcaggaa
     1321 gcgaggttac gacgtgtcgg ggagcaacag cgaaaaatgc acgccgcccg gggggcttac
     1381 gccaggcgtc ttgaggtaag ggatcgtatt gcaggtgccg gagccaccac cacggctgca
     1441 gggctggcaa tgggcgcacc agtgatggcg gcagtaaaaa gctataccag catggaagat
     1501 gccatgaaag gtgtggcaaa gcaggtcaat ggtctgcgtg acgataatgg caaccgcact
     1561 gcgcgttttt acgaaatgca gggtgccatc aaggctgcca gcgaacagtt gccgatggaa
     1621 aacggtgctg tggacttcgc cgcactggtt gaaggtggtg cgcgcatgaa cgtcgcaaac
     1681 cctgacgaca gctgggaaga ccagaaacgt gacctgctgg ccttcgccag cacggcggca
     1741 aaggcggcaa cagcctttga actgccagcg gatgaactgt cagaaagtct ggggaaaatc
     1801 gcccagctct acaaaatccc tacccgcaat attgagcagc tcggtgatgc gctgaactat
     1861 ctggatgata acgccatgtc gaaaggggcg gacatcattg atgtcatgca acgcctgggc
     1921 ggtgtggctg accgtctgga ttatcgtaaa gcggcggcgt tgggttccac cttcctgaca
     1981 ctcggcgctg cgcctgaggt tgcagccagt gcagcaaacg cgatggtgcg tgaattgtcc
     2041 attgccacca tgcaaagcaa gagtttcttt gaagggatga atctgctgaa actcaatcca
     2101 gaagtgattg aaaagcagat gacgaaggat gcgatgggaa ctatccagcg ggtgctggag
     2161 aaggtgaatg cgctgccaca ggataagcgc ctgtctgcca tgaccatgtt gtttggtaaa
     2221 gagtttggcg atgacgcagc gaaactggca aacaacctgc cggaacttca gcgccagtta
     2281 aaactgactg cgggcaatga tgcgctcggt tccatgcaga aagaatccga catcaacaag
     2341 gattcacttt ctgctcagtg gttgctggtc aaaaccggag cacagaacac cttcagcagc
     2401 ctgggggaaa cgctgcgcca gccgctgatg gatattctgt acacggtgaa aagcgtcacg
     2461 ggggcgttgc gtcgctgggt ggaagctaac ccggaactga cgggcactct gatgaaagta
     2521 gccgctgtgg tagctgccgt taccgtgggc ctcggcacct tagcggtggc gctggctgca
     2581 gtgctggggc cgctggcagt catccgtctg ggattctctg tgctgggtat caaaacgtta
     2641 ccttccgtta cggcaacagt aacccgaacc agcaacgcgt tgtcctggct ggctggcgca
     2701 ccactggcac tgctgcgacg cgggcttgct tcatcgggca acgctgcagg tttacttact
     2761 gcgccgttgt cgtctttgcg ccgcacggca tcactgacgg gaaatgtcct gaaaactgta
     2821 gcaggtgcgc cggttgcact tttgcggtct ggattatccg gtttacgtgc ggttgctgtg
     2881 atgtttatga atccactggc ggtactacgc ggcggactgg ctgccacagg cacggtgctg
     2941 cgtatgctcg catccggtcc actggcgatg ctgcgcgttg ccctgtatgc cgtctctggt
     3001 ctgttaggtg ctctgctcag tctgataggt cttgtggtta ctgcactggc gggtgtggcg
     3061 ctggttgtct ggaaatactg gcagcccatc accgcatttc tcggtggcgt ggtggaagga
     3121 ttcaaagcgg cggcaggtcc tgtcagtgcc gcgttcgaac cgcttaagcc tgtgttccag
     3181 tggattggcg acaaagtaca ggcgctgtgg ggctggttta ctgatctgct gacgcccgtt
     3241 aagtcgacct ctgccgaact gcagagtgca gcggcaatgg ggcgacgatt cggggaggct
     3301 ctggcggaag ggctgaatat ggtcatgcat ccgctggact ccctgaaatc cggcgtttcc
     3361 tggttgctgg agaaacttgg cattgtcagt aaagaggccg caaaggcgaa actgccggaa
     3421 agcgtgacgc gtcagcaacc tgcgacggtg aatgcagacg gtaaagtgat gatgccatcg
     3481 ggtggttttc cgtcatgggg atatggcttt gcggggatgt atgacagcgg cgggtatatc
     3541 ccgcgcgggc agtttggcat cgtcggtgaa aacgggccgg aaattgttaa cggcccggca
     3601 aatgtgacca gccggagaaa tacagctgca ctggctgccg ttgttgccgg aatgatgggt
     3661 gttgctgcta cgccagcaga gcttccaccg ttgcaccctt tggcacttcc cgcgaaaggc
     3721 ggtgaagcga tggtgagtcg tgcagccact gtgccgcccg ttcaacggat tgaggcaccg
     3781 atgcagatca tcattcaaac gcagccagga caaagtgcgc aggatattgc gcgggaggtg
     3841 gcccgccagc ttgatgaacg tgaacgcagg ctgaaggcaa aagccaggag taactacagc
     3901 gatcaggggg gatacgacgc atgatgatgg tgctgggatt gtacgtgttt atgctgcgca
     3961 ccgtgccgta tcaggagctg cagtatcaac gcagctggcg acatgcggca aacagccggg
     4021 taaaccgacg cccgtcaacg cagtttctgg gaccggacaa cgacatgctg acgctttctg
     4081 gtgttcttat gccggaggta acgggcggca ggctgtcgtt gctggctctg gagcagatgg
     4141 cagaacaggg aaaagcatgg cccctgattg aaggcagcgg cacgatttac ggcatgtatg
     4201 tgattgaggg actgaatcag actaaaacgg agtttttccg cgacggtatg ccgcgccgga
     4261 ttgagttcac cctgtcgcta aaacgcgtgg atgaatccct gtccgatatg ttcggtgatc
     4321 tcagtgcgca actgaataat ctgcagggaa cggcaacatc tgccttaagc gatatcagta
     4381 aaacggtggg agggctgctg tcgtgaattt cagctctgaa ctgcttaaca aaggcaacaa
     4441 aactccggca ttcagcatca gtattgaagg cagggatatc accactgtgc tggacaaccg
     4501 cctgatgggg ctgacgctga cggataaccg gggctttgaa gcggaccagc ttgatctgga
     4561 gctggacgac gccgatggaa aaatcgtgct gccgcgccgt ggggctgtca ttacgctggc
     4621 gctgggctgg aagggacagc cgcttttccc gaaaggggca ttcacggtgg acgagattga
     4681 acacactggc gcaccggatc gcctgactat ccgggcgcga agtgctgatt ttcgtgaaac
     4741 gctgaatacc cgccgtgaaa agtcgtggca caagaccacc gttggggaag tggtgaagga
     4801 aatagctgcg cggcacaaac tgaagatggc attgggtgaa gacttgtcgg ataaactcgt
     4861 ggagcatata gaccagacta atgagagtga cggcagtttt ttgatgcggc tggcgcgcca
     4921 gtacggtgct attgcgtcgg tgaaaaatgg caatctgtta ttcatccggc aggggcaggg
     4981 taaaagcgcc agcggtaaac cactaccggt gatcactatc acacgcaagg acggcgacag
     5041 tcaccgcttt accctggcag atcgcggagc ttacacgggc gtcattgcca gctggttgca
     5101 tacccgcgaa cccgcgaaga aagaaagcac cacggtgaag cgtaagcgca ggactaagaa
     5161 gcagaagaaa gagccggaag cgaagcaggg cgattacctg gtgggtacgg atgaaaacgt
     5221 gctggtactt aatcgcactt atgccaaccg gagcaacgcc gaacgggcag cgaaaatgca
     5281 gtgggaacgc ctgcaacgcg gcgttgcatc attctcgcta caactggcgg aagggcgggc
     5341 agatctctac acagaaatgc ctgtgaaggt cagtggcttt aaacagccgc tagatgatgc
     5401 ggaatggacc attacgactc tgacgcatac tgtcagcccg gataacggtt ttacgaccag
     5461 tctggagctt gaagtgagga ttgatgattt cgaaatggaa tgattcttcg caatggagaa
     5521 cttttaagtt tgcaaaatgg aataatgcgg tatcattatt gtgaatttag caaaaatggg
     5581 gagaactcga aaaatgatga tttgcccact gtgtggaagt gccgcccata ctcgcagcag
     5641 ttttcaggta tcttcattga ccaaagagcg ttacaaccag tgccagaaca ttaactgcag
     5701 ccatactttt gttacccatg aaacttttgt tcgttcgatt gcaacgccaa aagagtcaaa
     5761 tccggttcag ccgcatccaa tgaaatcagg acaggtggcg ctctctcttt gacgctgccg
     5821 ccattttgtc gccatcgtta aaaaacagtg cttctaacat catgatttta aaaggcataa
     5881 atttcagaca acaaaaaccc atcaaccttg aaccgaaatg gcggggttga tgggctccac
     5941 aaaatgggga catcaaagaa aagcagtggc actaattaag actgatgccc tgcggaaaag
     6001 ttctgcggtt gtgcaaaaaa atttcatttt cagggcaact tcagttttat cctaatcctg
     6061 gccataccat gacgatgatt gtccctgcca gcgtcagcag gacgttggcg attgcgtagg
     6121 tgcccgcata gcccagcgca gggatgttac tgcgagctgt atcactgatg atctccattg
     6181 ccggcgcgca ggtgcgtgcg cccatcattg cgccgaacaa cagtgcgcgg ttcattcgca
     6241 atacataagc accgaacaag aaacagataa ccacgggcac aagactgaca attaatccgg
     6301 caatcaacat ctgaccgcca atcgcgccca ggccgttatt aataccgcta ccggcgctca
     6361 gaccaacgcc tgccataaac accatcaagc cgaactcttt caccatgctt aatgcacctt
     6421 gcggaatgta accgaaggtc gggtggttag cacgcataaa gcccagcata attccggcga
     6481 ataacaaccc ggcagcgttc cccatgccga aactgaatgt gctgaactgg aaggtgatca
     6541 tcccgatcat cagcccaata acaaagaagg cgcaaaatgc cagcaggtca gtgacctggc
     6601 tgtgaatcga gataaagccg atgcgatcgg cgatggtttt tacgcgacgg gcatcaccgc
     6661 tgacttgtaa aacgtcacct ttgttaagca cgacgttgtc atctatcggc atctcaatct
     6721 ggctacgaat gatgcggtta aggaagcaac cgtgatcggt caacttcagt tgtgcgagac
     6781 gtttacctac agcgttatgg tttttaacga ccacttcttc agtgacgata cgcatgtcga
     6841 gaaggtcacg atcgaaaact tctttaccgt tacggaagct gggatcgagt cgggcatggg
     6901 cgtcgggata gcctaccaac gctatttcat cgcccatttg tagcacggca tcaccgtctg
     6961 gatttgccag aatcccgtta cgtcgaatac gttcaatgta gcagccggtt tgtcgataaa
     7021 tacccagttc acgcagattt ttgccgtcgg tccaggccac cagctccggg ccgacgcgat
     7081 aggcgcggat caccggtaaa taaaccttac ggttggcatc agtgtccagg ccacgttcgc
     7141 gggcgatttg ctgggcgctg gtctgtaagt cctgatgctg caatttcggc aagtaacgcg
     7201 caccaacaat caaactcacc agaccgatta aataggttaa ggcatacccg aggctcagat
     7261 tatccagtgc cagtgagagc tgcctgcttt ccatgccgga atgacgcagt gtatcgccag
     7321 caccgaccag aaccggtgtc gacgtcatag agcctgctaa cataccggcc gtcaggccaa
     7381 tatcccagcc aaacagctta cctaacccta aggcgatcac cagcgcactg ccaaccatca
     7441 ccagtgctaa cattaggtaa tttttcccat cgcgaaaaaa aatggaaaaa aagttcggtc
     7501 cggcttcgac cccgacgcag aaaataaaca gcataaagcc aagattaagc gcatcggtgt
     7561 taatgctgaa atgttgttgg cctaataaca gcgatacgac taaaacgcca atggaattac
     7621 ccagttggat cgaaccaagt cgtaactttc cgagacatag cccaagcgcg aggaccacaa
     7681 ataataacag aatgtaattc ccatttaaca attcggcgac gtttatattc acggaggcta
     7741 acttcttgtt tactagtaag ctgttgaaag aaatggtaat ttacgataat gttttttacc
     7801 agaattcagg gcgcagattc attcagcgca cctaaacgat agtaaagtaa caatatattt
     7861 tactagtgta atcacattag gtatcaacgg ctatatgaat tgcgttggcc tatattagca
     7921 tggaatgcga agcggcttta tcttactgaa cgccacactg gcgaaaaatg tgttcgatag
     7981 acgcagtgtc aggaggaacg agtgaaacat aaacaacgtt gggcgggggc aatctgctgt
     8041 tttgtcctct tcattgtggt gtgccttttt ctggcgacgc acatgaaagg cgcttttcgg
     8101 gctgccgggc atcctgaaat cggcttgctg tttttcattc ttcctggagc agttgccagt
     8161 ttcttttcac agcgtagaga agtcctgaaa cctctatttg gcgcaatgct ggcggcaccc
     8221 tgttcaatgc tcattatgcg gctgtttttt tcaccgacgc gctcattctg gcaagagctg
     8281 gcatggttac taagcgcggt gttctggtgt gcgctggggg cactgtgttt cttatttatc
     8341 agtagtttgt ttaaaccaca gcacagaaaa aatcagtaaa gccctcaacg cgagggcttg
     8401 tcagacgatc aggcgtccag attttctttc acccatgcag caaaatcggt atagccgccg
     8461 atatgttgct gatcgacaaa aatctgcggc acggtttcta cgggtttacc tgccttttgt
     8521 tgtagatctt ctttagtgat cccttccgca cgaatatcta catactgata ctgaaaatca
     8581 tcgcgttcat tgctcaattt ctcagccaga tcttttgcac gcacacagta agggcaaccc
     8641 gaacgaccaa aaataacggt ttgcattatt tctctcctca tagatttatg cctgtaatga
     8701 tcacgctaaa atgtattcgc tgaaagtagg tttaacctgt tgcattaatt gctaaaagct
     8761 ataactgtta aacacaatac agtgaaaagt tttagactga aggctcactt tgcagaggga
     8821 agcgtatgcg cgcgatcggt aaattgccta aaggcgtgtt gatactggaa tttatcggaa
     8881 tgatgctact ggcggtggcg ctgctgtcgg taagcgactc cctgtcgctg cctgagccat
     8941 tttctcggcc agaagtgcag attctgatga tttttctcgg tgttttgctc atgcttcccg
     9001 ctgcggtggt ggttattctt caggtggcaa aacgtcttgc cccacagctg atgaaccgtc
     9061 caccgcaata ttcacgttca gaaagagaaa aagataatga cgccaaccat tgaacttatt
     9121 tgtggccatc gctccattcg ccatttcact gatgaaccca tttccgaagc gcagtgtgag
     9181 gcgattatta acagcgcccg tgcgacgtcc agttccagtt ttttgcagtg tagtagcatt
     9241 attcgcatta ccgacaaagc attacgtgaa gaactggtga cgctgaccgg cgggcaaaaa
     9301 cacgtagcgc aagcggcgga gttctgggtg ttctgtgccg actttaaccg ccatttacag
     9361 atctgtccgg atgctcagct cggcctggcg gaacaactgt tgctcggtgt cgttgatacg
     9421 gcaatgatgg cgcagaatgc attaaccgca gcggaatcgc tgggattggg cggggtatat
     9481 atcggcggcc tgcgcaataa tattgaagcg gtgacgaaac tgctgaaatt accgcagcat
     9541 gttctgccgc tgtttgggct gtgccttggc tggcctgcgg ataatccgga tctcaagccg
     9601 cgtttaccgg cctccatttt ggtgcatgaa aacagctatc aaccgctgga taaagacgca
     9661 ctggcgcagt atgacgagca actggcggaa tattacctca cccgtggcag caataatcgc
     9721 cgggatacct ggagcgatca tatccgccga acaatcatta aagaaagccg cccatttatt
     9781 ctggattatt tgcacaaaca gggttgggcg acgcgctaaa accgccacgt cgatgtatga
     9841 tacgcgggct tttgaccagg tctgacagag aggtgcaggg tgaaaattgc catattgtcc
     9901 cgggatggaa cgctctattc gtgtaagcgg ctgcgtgaag ccgctataca gcgcggtcac
     9961 ctggttgaaa ttcttgatcc gctttcttgc tacatgaaca taaatcctgc ggcgtcttct
    10021 attcactaca aaggccgcaa gttaccccat tttgacgcag tgatcccgcg tattggcacc
    10081 gccattacct tttatgggac ggcggcactg cgccagttcg agatgctggg gagctatccg
    10141 ctcaatgagt cggtcgccat tgcccgggcg cgtgacaaat tgcgttccat gcaactgctg
    10201 gcgcgtcagg gcatcgacct gcctgtcacg ggcattgcgc attcgccgga tgataccagc
    10261 gatttaatcg acatggtcgg tggtgcgccg ctggtggtca agttggttga aggcacgcag
    10321 ggaattggcg tcgtgctggc ggagacgcgt caggcggcgg aaagcgtgat tgacgctttc
    10381 cgcggtctga acgcgcatat tctggtgcag gaatatatca aagaggcgca agggtgcgat
    10441 atccgctgtc tggttgttgg cgatgaagtg gtcgctgcga ttgaacggcg ggcgaaagag
    10501 ggcgattttc gttccaattt gcatcgtggc ggcgcggcaa gcgtcgccag tatcacacca
    10561 caggagcgtg aaatcgcgat aaaagccgcg cgaacgatgg cgctggacgt tgctggtgtg
    10621 gatattctgc gtgctaatcg cgggccgttg gtgatggaag tgaatgcgtc gccggggctg
    10681 gaaggaatag aaaaaaccac tggtatcgac atcgcgggta aaatgatccg ctggatcgaa
    10741 cgctacgcta cgacagaata ttgcctgaaa acgggtggtt agtcgcaatc acattactga
    10801 tcatggtttt gcctgcgctt tttgcgtaag ctgtgccggt ctttttatcg aaagaggttg
    10861 tacaaaatta tgacatcgct ggtcgttcct ggtctggata cgctgcgtca atggctcgat
    10921 gacctgggga tgagtttttt tgaatgtgat aactgtcagg ctctgcatct gccccatatg
    10981 cagaatttcg acggtgtctt tgatgccaaa atcgatctga tcgataacac gatcctgttt
    11041 tctgccatgg cggaagtccg accttcagcc gtattgccgc tggcggcgga tttatctgcc
    11101 atcaatgcca gttcgctgac cgtgaaagca tttcttgata tgcaggatga taatctgcca
    11161 aagctggtgg tttgccagtc tttatccgtt atgcagggcg taacctacga gcagtttgca
    11221 tggttcgtgc gtcagagcga agagcagatt tcgatggtca ttcttgaagc taatgcccat
    11281 caactgctgt taccgactga cgatgaaggg caaaacaacg ttaccgaaaa ctatttcctc
    11341 cactgataac tcctttcgag cacgcagtcg ctggtgcagt gactgcgcac tgcaaaatta
    11401 tctgctgttt ttaacctttt cttaaagatt atttcacttc tcttgtgccg atttggcttt
    11461 atcacataga gcaaatacgc ataacaattt gttaaatacc gttttttaat ccgagctata
    11521 gtctcaaacc ctggctaaag ttattcttgc gatgctttta tatagcgagc agtgctggcc
    11581 gggagagagt tatcttttct tacaccgcgc cgataaaaaa tatgcacgtt tattgcatat
    11641 cttttagtat gacaactttt gttcgtttgt taacgaactt tcagaaggaa agagatatga
    11701 ccgccttaaa taaaaaatgg ctttcgggtc tggttgcggg tgctctgatg gccgtctctg
    11761 tcggcacgct cgcggctgaa caaaaaacac tccacattta taactggtct gattatatcg
    11821 cccctgacac ggtggccaat tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt
    11881 tcgactctaa cgaagtactg gaaggcaaat taatggcggg tagcaccggc tttgacttgg
    11941 tggttccatc tgccagcttt cttgaacgcc agttgactgc gggagttttc cagccgctgg
    12001 acaaaagcaa attgccggag tggaagaatc tcgacccgga gctgctgaag ctggtcgcca
    12061 aacacgatcc cgacaataaa tttgctatgc cctatatgtg ggcgacgacc gggattggct
    12121 ataacgttga taaagttaaa gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact
    12181 tgatcctcaa acctgaaaat ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg
    12241 cgccagaaga agtttttgct accgtgttga attatctcgg caaagatccc aacagcacta
    12301 aagcggatga ttacaccgga ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc
    12361 gttatttcca ttcatctcaa tacattaacg acctggcaaa cggcgatatt tgcgtcgcta
    12421 tcggctgggc aggtgatgtc tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag
    12481 tgaatgtctc gttctcgatt ccaaaagaag gggcgatggc gttctttgat gtattcgcca
    12541 tgcctgcgga tgccaaaaac aaagacgaag cctatcagtt cctgaattac ctgctgcgcc
    12601 cggatgtagt agcgcatatt tccgaccatg tgttctatgc caacgccaat aaagcagcca
    12661 cgccgctggt gagtgcggaa gtccgtgaga acccgggtat ttatcctccg gcggacgttc
    12721 gtgcgaagct attcactctg aaagtacagg atccgaaaat cgaccgtgtg cgcacccgcg
    12781 cgtggaccaa agtgaagagc ggaaaataat ccgcagcctt agatgccggg cgggcgcacc
    12841 acacccgccg gcaattcgca ccatcatggt gcgcttgcac acattcaatg ccggagagca
    12901 gccgtgaatg acgctatccc tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta
    12961 ttagaaatcc gcaacctcac caaatcctac gatggtcagc atgcggtcga tgatgtcagc
    13021 ctgaccatct acaaaggtga aatcttcgcg ctgctgggcg cttccggctg tggcaagtcc
    13081 acgctgctgc gtatgctggc aggttttgaa caaccttctg ccgggcagat aatgctcgat
    13141 ggcgtcgatt tgtcacaggt gccgccttac ctgcgcccca tcaatatgat gtttcagtct
    13201 tacgcgctgt ttccgcatat gaccgtggaa cagaacatcg cttttggcct gaaacaggac
    13261 aaactaccga aagcggaaat tgccagtcgg gtccatgaga tgctcggcct ggtgcacatg
    13321 caggagttcg ccaaacgcaa accgcatcag ctttccggtg gtcagcgaca gcgtgtggcg
    13381 ctggcccgaa gccttgccaa gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg
    13441 ctggataaaa agctgcgtga ccggatgcag cttgaagtgg tggatattct ggagcgtgtc
    13501 ggcgtgacct gtgtgatggt cactcacgat caggaagagg cgatgacgat ggcgggacgt
    13561 atcgccatta tgaatcgcgg gaaatttgtc cagattggcg agccagaaga gatctacgaa
    13621 catccaacca cccgctacag tgctgaattt atcggttcgg ttaatgtctt tgaaggtgta
    13681 ctcaaagagc gtcatgagga tgggcttgtg ctttattcgc ctgggctggt gcatccactg
    13741 aaagtcgacg cggatgcctc ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg
    13801 gaaaaaatca tgctttgcga agagccgccc gccaatggtt gtaacttcgc ggtgggggag
    13861 gtgatacaca ttgcctatct cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg
    13921 cagatgatta gcgcccagct acaaaacgcc catcgtcatc gtaaagggtt accgacctgg
    13981 ggcgacgaag tgcgtttgtg ctgggaagtg gacagctgtg tggtgctgac ggtttaagga
    14041 gcaaagatga atacacttga acctgctgcc cagtcgaaac cgccgggcgg atttaagctg
    14101 tggttgtcgc agctgcaaat gaagcatggg cgcaaactgg tcattgcgtt gccatatatc
    14161 tggttgatct tgctgtttct gctgccattt ctgattgtct ttaaaataag cctggcggag
    14221 atggcgcgcg ctattccacc ttataccgaa ctgatggagt gggctgacgg gcaactgtcc
    14281 atcactctta atctcggtaa ttttctgcaa ctgaccgacg atccgctcta tttcgatgct
    14341 tatctccagt cgctacaggt ggctgcgatt tcgacatttt gctgtttact gatcggctat
    14401 ccgctggcgt gggcggtggc gcacagtaag ccttcgaccc gtaatatttt attactactg
    14461 gtgatcctgc cgtcgtggac ctcgtttctg atccgcgttt atgcctggat gggaatatta
    14521 aaaaacaacg gtgtgctgaa taattttctg ctgtggctgg gggttatcga tcaaccgctg
    14581 accattcttc ataccaatct ggcagtttat attggcattg tttacgccta cgtgccgttt
    14641 atggtgctgc cgatttatac cgcgttgatt cgtattgatt attcgctagt ggaagcagcg
    14701 ctggatctcg gtgcacgacc gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa
    14761 ggtgggatta ttgccggatc gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc
    14821 ccggaactgc tcggtggccc ggacagcatc atgatcgggc gcgtgctatg gcaagagttt
    14881 ttcaataacc gcgactggcc ggtggccgcg gcggtagcga tcatcatgtt gctgctgcta
    14941 attgtgccga taatgtggtt ccacaaacac cagcaaaaaa gcgtgggaga acacggatga
    15001 ataatttacc ggtagttcgt tcgccctggc ggattgtgat tttgctgctg ggctttacct
    15061 ttctctacgc gccaatgctg atgctggtta tctattcgtt taacagctcg aagctggtga
    15121 cggtgtgggc cggctggtca acgcgctggt atggtgagtt attgcgcgat gacgcgatga
    15181 tgagtgcggt tggtttaagc ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc
    15241 tcgggactat tgcggcggtg gtgctggtgc gctttggcag gtttcgcgga tcaaatggct
    15301 ttgcctttat gatcacagcg ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt
    15361 tgttattatt cgtcgcgctt gctcatgcca ttggctggcc tgcggaccgc ggtatgctta
    15421 ccatctggct ggcgcatgtc actttctgta cggcttatgt ggcggtcgtt atttcgtcgc
    15481 gtctgcggga actggatcgc tcgatagaag aagcagcgat ggatctcggt gcgacgccgc
    15541 tgaaagtgtt tttcgtcatt acgctaccga tgatcatgcc tgcgatcatt tctggctggt
    15601 tactggcttt tactttgtcg cttgatgatc tggtgatcgc cagctttgtt tccgggccgg
    15661 gagccaccac gttaccgatg ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa
    15721 tcaacgccct tgcaacgtta attctcggtg cggtcggaat tgtcggattt atcgcctggt
    15781 atctgatggc tcgcgcagaa aaacagcgga tacgcgatat ccagcgtgca agacatggct
    15841 gaagacacta aaatttgcca acctggctac ataatgccgc gcatgccgcg gcattgtttt
    15901 catggaagac gaaacgttgg gattttttaa gaaaacatct tcatctcatg ctcgcctgaa
    15961 tgtgcctgcg ctggtgcagg tagcggcgct cgccattatt atgatccgtg gcctcgacgt
    16021 gctgatgatt ttcaatacgc tgggcgtgcg cggtattggc gagttcattc atcgcagcgt
    16081 acaaacctgg agcttaacgc tggtattttt aagcagtctg gtgctggttt ttatcgagat
    16141 ctggtgtgcg ttttcactgg tgaaagggcg tcgctgggcg cgctggctat atctgctgac
    16201 acaaatcacc gccgcaagtt acttgtgggc ggcttcgctg gggtatggtt atccggagct
    16261 gttcagcatt cccggtgaat caaaacgtga aatcttccat agcctgatgc tgcagaagct
    16321 gccggatatg ctcatcctga tgctgctgtt cgttccctcg accagtcggc ggttcttcca
    16381 gttgcaataa tgtgtataat cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg
    16441 cactttacga cgcgggtcgc tgtcgttcct gtcagtggat aacgcagccg attccagagc
    16501 aactctccgc taaaaccgcc gatcttaaaa atctgctcgc cgattttccg gttgaggaat
    16561 ggtgcgcgcc ggtgtcaggc ccggaacaag ggtttcgtaa taaagccaaa atggtggtga
    16621 gtggtagcgt tgaaaaacca ctgctcggta tgctgcatcg cgatggcacg ccggaagacc
    16681 tttgtgactg cccgctttat cctgcctctt ttgcgcccgt ttttgcggcg ctaaaaccgt
    16741 ttatcgcccg tgcggggtta acgccctaca acgtggcgcg taagcgtggc gaactgaaat
    16801 acattctgct gactgaaagc cagagcgatg gcggcatgat gctgcgcttt gtactgcgtt
    16861 ctgaaaccaa actggcacaa ctgcgtaagg cgctgccgtg gttacaggaa caactaccgc
    16921 aactgaaagt tattaccgtc aatattcagc cggtacatat ggcgattatg gaaggggaga
    16981 cggagatcta cctgactgaa caacaggcgc tggcagagcg ttttaatgac gtaccgctgt
    17041 ggatccgtcc gcaaagtttc ttccagacta atccggcggt cgccagccag ctgtacgcta
    17101 ccgcgcgcga ctgggtacgg cagctgccag ttaaacatat gtgggatctg ttctgcggtg
    17161 tggggggctt tggtttacac tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg
    17221 cgccagaggc cattgcctgt gcaaagcagt cagccgctga actgggctta actcgtttac
    17281 aatttcaggc gctggactcc actcagtttg ccactgctca gggggaggtg ccggagctgg
    17341 tgctggttaa cccgccgcgc cgcggcattg gtaaaccgct gtgtgattat ctctcaacga
    17401 tggcaccgcg ttttatcatc tactccagct gtaacgccca aactatggcg aaagatatcc
    17461 gcgaactgcc tgggtttcgc atcgaacggg tacagctttt tgatatgttc ccgcacaccg
    17521 cgcactatga agtgctgacg ctgctggtga agcaatctga agccgcaggt gcggcttcag
    17581 attgctgatc aggtgcgctt tgttcatgcc ggatgcggcg tgtacgcctt atccggccta
    17641 caaaatcatg ctaaatcaat acattgcaga gttgatgtgg gccagataag cgcagcgcat
    17701 caggcaatct tacttttgtc atcagtctca attactgcgg gaaccactgg tcattgattt
    17761 tctgatatgt accgtcagct ttaattgctg ccagcgcgtt attcagtttt tccagcagag
    17821 ctttgttatc cggacgtaca gcgatgccca gaccggtgcc gaagtattgc ggatcggtca
    17881 ctttttcagt agcaacgccc agttgcggat tggttttcag ccattcgttt accaccgctg
    17941 tgtcaccaaa taccccatca atacgaccat ttttcagatc gataaaggca ttctgataac
    18001 tgtcatagga gacagttttc acttcggggt gctgatcctg aatatatttc tggtgcgtgg
    18061 tgccgttttc catcccaata cgtttgcctt tcagatcggc aaacgttttg taggtatctt
    18121 ttttggcaat cacgacggct gagttttcat agtagggcgt ggtaaacgat acctgcttgc
    18181 tacgctcggg cgtgatatcc ataccggaga ttacggcgtc atattttctg aatttcagtg
    18241 acgggatcag gctgtcgaac gcgtgattag taaaagtaca ttctgcctgc atttgtttgc
    18301 acaaggcttt tgccagatcg atatcaaagc cgacaatctc attattagca cctatagatt
    18361 caaagggtgg ataggtggct gaaacgccaa aattgatttt ctctgcggca gaagcaccga
    18421 aagtaaagga agcaagtaaa gcggcaagaa ctaacttttt catgatggaa ctcccgtctg
    18481 tcaatcttat gatttttggc cgtgtctgcg gcatgggata acaatgccat caagtgaatt
    18541 tatatgcaat aaatatgatt aaataattta aatgaaataa aaaacgacgg ataacttagt
    18601 gagttgtccg tcttcattat aagaatttat gcactatgta ggccggataa ggcgtccacg
    18661 ccgcatccgg cacaggcacc gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac
    18721 ctacaaaccc cccggcgaat gtacgcagct acattaattc cgccgttcga atgccagtgc
    18781 tttgcgctcg atcagacgca tcatcagcgt cagcaggccg ttgacgacca ggtaaataat
    18841 ccctgccgca ccgaacacca ttacatcgta ggtgcgtccg tacaacaact ggctgtatcc
    18901 catcacttcc atcagcgtaa tggtgtaagc cagagaggta cttttgaaca ccagcaccac
    18961 ttcgttggaa taagaagaga gcgagcgttt aaaggcatac ggcagcagga tcgccagcgt
    19021 atcttttttg ctcattccca gggcgctaca ggactgccac tgaccttctg ggatcgcacg
    19081 aattgcaccg taaaacagct gcgtggtata cgccgcactg ttcagcgata acgcaatcag
    19141 cgcacataac cacggttctg acaacaaatg ccacagtgcc ggatactcct gcaaagtcgg
    19201 aaactggccc ggcccgtaat aaatcaggaa gatctgcacc agcagcggcg taccggtaaa
    19261 cagcgtgata taaccccgca ccagccacac cagcaccggc gttttcagcg tcaggatgat
    19321 ggtaaaaatc aatgccagaa tcagtgccac aatcagcgag gcaacggtta gcgtcaggct
    19381 ggtgtgtagc cctttcatca gttcgggtaa atactcaaac attagctggg cctccgctca
    19441 aaacgtgtcg cgcgcaggtc aatgcgtttg agaatgtact gactgagcag ggtgatcacc
    19501 aggtaaatcg ccgccgccac aatataccag gtaaatggtt cctgggtacg agtagcgatg
    19561 ctttttgttt gcagcattaa atcattcaca ctaatcaaac tgaccagcgc ggtatctttc
    19621 agcagcacca gccactggtt accgaggcca ggcagcgcat gacgccacat ctgcggcatc
    19681 accagacgga aaaagatagc cgatttcgac agccccagcg cctgaccaga ctcccactga
    19741 cccaccggca ccgctttcaa cgcgccccga agcgtttgcg aggcataggc ggcatacagc
    19801 agtgacagag cgatgacacc acaaaggaac gggctaacgt cgaagttctc aatgtccatc
    19861 tgcactggga tctgcacgaa cccaagattg atagtgaagc catccgaaag cgtcagcagc
    19921 agctgcgagg agccaaaata gataaacagc accaccagaa tttctggcag gccacgcaga
    19981 atggttacca gcgctgaacc tgcccacgcg acaggacgcc atttttccga ctcccatacc
    20041 gcaaagaaca tcgccagcgc cagcccgaca atcaatgcac aaacggcaag gccgacggtc
    20101 atcccggcgg cgcttgctaa aggaaaaaat tcattcatca ggaattactt ctggaaccat
    20161 ttgttgtaga tggtttcgta agtgccatct ttcttcactt tttccagcgc agtgttgagt
    20221 ttctgctgca gctcagtgtt gccctgacgt accgcgatgc cgaggccagt gccgaagtaa
    20281 tctttatcgg tcactttgtc gcccaccgcc gccagtttcg ggttatcttt cagccactca
    20341 gtgaccactg cggtgtcacc gaagacgccg tcgatacgcc cgttttgcag atccagtttt
    20401 gcgttctggt agctgttata cggaacggta gtgatttccg ggtgcttatc cataatgaat
    20461 ttctggtgtg tcgtcccgtt ctgtacgccg acttttttgc ctttcagctg atcaacactg
    20521 gtgtatttgc cttgctgacc cacaaacagg gcagagttgt catagtacgg ggtggtaaac
    20581 agcacctgct tttcacgctc cggagtgata tccatgcccg ccatcacggc ttctacgcga
    20641 cggaatttca ggcttgggat caggctgtca aacgcctggt tagagaaagt gcaggttgca
    20701 tcaatctctt tacacagcgc ttgtgccagg tcgacgtcaa aaccaacgat ctggttgttt
    20761 gcatcaatcg attcaaacgg aggataggat gcttcggtag caaaacgaat ggtttcggca
    20821 gctgtggcgg aaagactaaa acctgcaatt aacgcggcaa tcagaacttt tttcattgtt
    20881 gttatcccga atcttagtga gagagatagt ttttaaatgc ttcggtttgc ggctcggtaa
    20941 agcagctcgc gtcgccttgt tctacgatat gaccattttc catatacacc actcggctgg
    21001 cggttttacg cgccacttca acttcgtggg tgacgatcac ctgggtaata ttcgtttctg
    21061 ccagctcacg aatgatgctg acgatttgtg ccgtaatttc cgggtccagt gcggcggtcg
    21121 gttcatcgaa cagcagtacc tgcggttcca tcatcaacgc acgggcaata gcaacacgct
    21181 gctgctgacc accagaaaga tgcagcgggt aacgatcgct ataaggtttg agacgcagac
    21241 gttccagcag tttttctgca cgggccagcg cctgatcttt actcaacccc agtacacggc
    21301 agggcgcttc aatcaggttt tgctgcacgg tcagatgcgg ccacaggttg tattgctgaa
    21361 acaccatgcc aacgttacga cgcaaatcgc gaatcgcttt gtcagagggt gttttggtga
    21421 aatcgaaatg gttgcctgca atgttgagcg taccggagcg cggcatctca agcagattga
    21481 gtacacgcag cagcgagctt ttacccgcgc cgctggggcc aagtaacacc agcgtttcgc
    21541 cctgtgggca atccagcgtg atatcgaaca gcgcctgatg cgcgccgtag aagcaattaa
    21601 tgccgtttaa ttgaatactc attgacactc gtatactggc agtctgatag ctattgaggt
    21661 cgaagatagt acctttgaca gaataattat gcaatatttc tgctttaaaa gttaaaagca
    21721 aagcgcatta ttcaataaac atagcacaaa ataacggggg cggtggtcgg cgagcataaa
    21781 tgtcggcatt cctcacgaaa tgccggacaa tttacggggt ttattggttg atcaaggcgt
    21841 tagcgattct cgatggactg acggagcgta cccgccgtgg catgaacgct gccgcctaag
    21901 tagcgtacat cgtcgatcac ccaacactgg ccttcctgaa tcattaacac ttcatcctgc
    21961 cagctctgat caccctgttt tagatccaca cgcagaggaa tatttcgcgc atcacgattc
    22021 gggattgttg aggcactggc aacacgggcg ctctctggca aggttgtgcg actggagaat
    22081 ggatcgttgg tcagtagttc ccgatggtta ttatcccggg aggcatcgct aagcagtgtc
    22141 gccagtttgt cgctcagata agggcgcaag gcggtgatgt cgttgctgcg gtgcaaaatg
    22201 cggtagtcat aaaattgctg ggccacgtta cccgggcctc cttcaacgca aggaccactg
    22261 cgtgtgccgt tatctttata agctggagtg actgtggtgc aggcactgag gagcagtgcg
    22321 caggggataa gcattgtcaa tttgctgtag cgcataatga tttccttata agcgatcgct
    22381 ctgaaagcgt tctacgataa taatgatatc ctttcaataa tagcgtatca gtctgataat
    22441 gcttttgaga tcgaaggctt agcaaacaag gagatcgatc atgcaatttt ctacaacccc
    22501 aactctggaa ggccagacca tcgttgaata ttgcggtgtg gtgaccggcg aagcgatttt
    22561 aggtgccaat attttccgtg atttctttgc cggtatccgc gatatcgttg gcggacgttc
    22621 cggtgcgtat gaaaaagaac tgcgtaaagc acgggagatc gcctttgagg aattaggctc
    22681 ccaggcgcgg gcgctggggg ccgatgccgt cgtcggtatt gatatcgact acgaaacggt
    22741 cgggcaaaac ggcagtatgc tgatggttag cgtcagcggt acggcggtga aaacgcgtcg
    22801 atgagaagag tcttctggct ggtcgctgcc gctctgttat tggcagggtg tgcaggcgaa
    22861 aaaggcattg tcgagaaaga gggatatcag cttgataccc gacgccaggc gcaggcggcg
    22921 tatccgcgca ttaaagtgct ggtgatccac tacaccgcag atgattttga tagctcgctg
    22981 gcgacactga ccgataagca ggtcagctcg cattatctgg tccctgcggt accaccgcga
    23041 tacaacggta aaccgcgcat ctggcaactg gtgccggaac aagaactggc ctggcatgcg
    23101 gggattagcg cctggcgcgg ggcaacgcgc cttaacgaca cctctattgg cattgagctg
    23161 gaaaaccgtg gctggcaaaa atcggccgga gtgaaatatt ttgccccgtt tgaaccggca
    23221 cagattcagg cgcttattcc actggcgaaa gatattattg cccgttatca catcaagccg
    23281 gaaaacgtag tggcacatgc ggatatcgca ccgcagcgca aagacgatcc ggggccatta
    23341 tttccctggc agcaactggc gcagcagggg attggtgcct ggccggatgc gcagcgggtt
    23401 aacttttacc ttgccgggcg cgcgccgcac acaccggtag ataccgcgtc attgctggag
    23461 cttttggcgc gctacggtta tgacgttaaa cctgatatga caccgcgcga gcagcggcgc
    23521 gtgattatgg cattccagat gcatttccgc ccgacgttat ataacggcga agcggatgca
    23581 gaaactcagg cgattgccga agcattgctg gagaaatacg ggcaggatta gcgcggcagt
    23641 tttccgtggt cgcgtagcca ggcggcagtt ttctcgatac cttcatccag ggtgatgacc
    23701 ggctgataac ctaactcttc ctgcgcacgc gtaatatcca gcgtaaagtc aaaattcaac
    23761 ttggagacgc cgtagtgggt cagcggcggc tcttttgctg acttgcggaa acgctccatg
    23821 ctgcgggcga tcatatccag catcgggtag gggacggaac gaatacgaca gtcaatattc
    23881 aactcgtcga tcagcttctg cacgatgctg cacagtgtgc gatgctcgcc gttggtgatg
    23941 ttgtacacac gcccggaagg tagcttatcg caggcttcct ggcttgccag ccacattgcg
    24001 tgcacggcat tttcatagta ggtcatatcg accagcgcac tgccgccatg cggtaacaga
    24061 atactgccgt agtggtgcat catatgcgcc agacggggaa taaagacttt atcgtgcggt
    24121 ccgaacagac tttgtgggcg cagaatagta aagcgcgttt gtggattcgc ctgcgaaaac
    24181 atattgatca cttcttcgct ggctgctttg ctgcgggcaa actcgttggc gaagcggtga
    24241 gggcgaaaat cttctttaat atcgcgatgg tggtgataat cgaagtacag ggagggggaa
    24301 gagatatgaa taaagttacg cacaccccag gcgacagccc attcacccaa gcggcgagtg
    24361 gcgcgaacgt tagccagatc gaaagcctgt tgtgtccccc agggtgaggt aaagctggag
    24421 cagtgccaca gcgtatcaat gcccgcgagc atcactttag cttgtgatga aaccagctcg
    24481 gtcagatccg ccggaacaaa ctctgcgccc attttttcca gcaatttgcc cattgcctcg
    24541 ttgcgaccgg tcgctcgcac gctgatgcct ttctggcata aaaactctac cgcgtttcga
    24601 cctaagccgc tggtggcgcc ggtaaccagt accttcatat caatccactg ttgttgagaa
    24661 aataacgtgc gcattcttcc gtgatttccc ccatgatgca atgggaaaca tgaaagaata
    24721 acgcaggttt tgtcgattaa tctgtgcttt gttctgccag tctggcgatt tgttttgcca
    24781 ttccgcgaaa aataaacaga tgcgcgggga tcatcaataa ccagtaaaac agccccggca
    24841 taccgtgcgg atgccagaaa gcgcggacat cgatagtacg atagtcgcct ttatcttcca
    24901 ggctaaaaca cagtcgtccc agccccggcg ctttcatgcc aaataacaac gtaagttgtt
    24961 tttccggttc aacgacaatc actttccagc tatctaccgc atcgccagtc tgtaaatatt
    25021 cgcgctccgg gcggcctttc gccagcttat gaccgatcgc gcggtccatc aacgcccgtg
    25081 tctgccacaa aatattgcca aagaaataac gctctttacc gccgatttgg ttcactacct
    25141 gccataaagc agcaaggctg gcggacgttt taacggtaaa ccccgcctgt ttggcaaaat
    25201 aaccgtactc cggtcgccag cgggcaaagg cctgagcgtc gtagccccag tcgctggagt
    25261 tgaccagttt ttcctcctct ttcaacgtgc tacgtaccgc gtcatcgaaa gcgatcagcc
    25321 gttgtgggat gagtgcacgt agcgcggtat catccgccag cagatcgtgt ttcagcccct
    25381 gaatcaacgc cctggcggtg gtgggcggta cggaagtaat cacattgaga aaccacaccg
    25441 aaatccagcg ggtggggagg gggatgggga tcaaccagcg gcgcttgccg ctcaccgcca
    25501 taaaatgttc aaactgttgc tgataactga gcacctctgg tccggcggct tcgaagatgc
    25561 ggtgttcgct ggccggatga tctaacaacg ccaccagata gtgcagcaag ttttccagcg
    25621 cgatgggcgt ggtgcgtgaa cgtacccagc gtggcggcgt taacactggc aggttgtaga
    25681 ccatatcgcg catgacttcg aacgccgctg aacctgcgcc aacgataatt ccggctcgaa
    25741 gttcggtcac aggtacattc gcttcacgaa gaatgtccgc cgtagcctga cgagcacgca
    25801 gatgatccga ctgctcatgt ggcggggcct gcaacgaact gagaaagatt aattgcttaa
    25861 ctggtacttc acgtagcgca tcgcggacgt tgagagccac ctggcgctcc tgagcgataa
    25921 aatcgccgcc ttcgcccatg ctgtgcacca gaaaatagac ggtatcgata tcctgcaaca
    25981 gggccggaag gttatccggc cagctgagat cgactttatg gcaactgacg tttgccagtt
    26041 gcagctttgc aagcctgtcg acatgacgtg ccgccgccag gatctgatgc ccttgctggc
    26101 tgagtgtgcg caccagatgc tgaccaatgt agccactggc accgagaact aaaatgcgtt
    26161 gcggcacgtc tctctcctta acgcgccagg aatgcgcgcc agtgggcggc gacttccgcc
    26221 agttgttcgc gcgagacgtc aagatgcgtc accaggcgga caatcggcga ggcgttaatc
    26281 agcacgtttc tcactttcat gtattcgcct aacgcggcag cattttcttc cccgacgcga
    26341 acaaacagca tattagtgtc ctgacgcatc acatccgcgc ctgcttcacg cagttgctcc
    26401 gccatccagg cggcgttgtc gtgatcttcc tgcaaccgtg cgacgttatt tttcagggca
    26461 tacatcccgg cggcagccag aatcccggac tgacgcatcc cgccaccggc cattttccgc
    26521 cagcggatgg cacgtttaat gtaatcacga ttaccgacga gtaatgaacc gacaggcgtc
    26581 ccaagacctt tcgacaggca aatggtgaac gaatcacaat attgcgtgat ctctttcagt
    26641 tcgcagccgt aagccaccac ggcattaaag atgcgcgcac cgtcaacatg cagcgccaga
    26701 ttgcgctcgc gggtaaattc ccatgcttct ttcaggtatt cacgcggcag cactttgccg
    26761 ttgtgggtgt tttccagact gagtaatttg gtgcgggcga aatggatatc gtcgggtttg
    26821 attttcatcg ccactttatc cagcggtagc gtgccgtcgg cagccgcgtc gatgggttgc
    26881 ggctgaatgc tgcccagcac cgctgcgcca ccggcttcaa acagatagtt atgcgcggcc
    26941 tgaccgacaa tatactcttc gccacgttcg cagtgactga gcagagcgac caggttggcc
    27001 tgagtgccgg taggcagaaa aatggcggct tctttaccgg aaagctctgc tgcatagtcc
    27061 tgcagagcat taacggtagg gtcgtctccg taaacgtcgt ccccaaccgg ggcggccatc
    27121 atcgcttcga gcatggcgcg gctcggtcgg gtaacggtat cactgcgtaa atcaatcatg
    27181 gcatgtcctt attatgacgg gaaatgccac cctttttacc ttagccagtt cgttttcgcc
    27241 agttcgatca cttcatcgcc gcgtccgctg atgattgcgc gcaacatata caagctaaat
    27301 cctttggcct gttcgagttt gatctgcggt ggaatggcta actcttcttt ggcgaccacc
    27361 acatccacca gcaccggacc gtcgatggag aaggcgcgtt gcagggcttc atcaacttca
    27421 gacgcttttt ctacacggat acccgtaatg ccgcacgctt cggcaatgcg ggcaaagttt
    27481 gtgtcgtgta gttcggtgcc gtcagtcaaa tagccaccag ctttcatctc catcgccaca
    27541 aagcccagca cgctgttgtt aaagacgaca attttcactg gcagtttcat ctgcactact
    27601 gagaggaaat cgcccatcaa catgctaaaa ccgccatcgc cgcacatggc gaccacctga
    27661 cgttctggct ctgtcgcctg cgcacccagc gcctgcggca tggcgttagc catcgaaccg
    27721 tggttaaacg aacctaacag gcgacgcttg ccgttcattt ttagataacg tgccgcccac
    27781 accgttggcg taccaacgtc acaggtgaaa atagcgtcat cggcggcaaa atgactaatt
    27841 tgctgcgcca gatattgcgg gtgaatggct ttctcgctcg gtttagctaa atcgtccagc
    27901 cctttgcggg cgtcgcggta atcttccagc gctttatcca gaaacttgcg atcggctttt
    27961 tcttccacca atggaagcaa tgcacgcaga gtcgacttga tatcgccgac cagtgccata
    28021 tccaccttgc tgtgagcgcc gatgctggct gggttgatat caatctgaat gattttggca
    28081 tcggtcgggt agaaggcgcg gtagggaaat tgcgtgccga gtagcactaa cgtgtcggcg
    28141 ttcatcatgg tatggaaacc tgacgagaag ccgattaacc cggtcattcc aacatcatac
    28201 ggattatcgt attcgacatg ttctttaccg cgcagggcat gaacaatagg cgctttaatt
    28261 ttcccggcaa actcaactaa ctctttatgc gcccccgcgc agccgctgcc acacatcagg
    28321 gcgatattgc tggaataacg cagcagttgc gccagtttgc gtaactcttc ttcttccggc
    28381 gtcacgactg gttgtggcgc atgataccag tgcgtggttg ccccttctgg cgcaggtttt
    28441 aacgccacgt cgcctggtaa cacgacaacc gaaacgccac ggttaagcac cgctttgcgc
    28501 atggcaatcg ccagtacttg tgggatctgc tccgggctgg aaaccagctc gcaatagtga
    28561 ctacattcgc ggaatagctc ttgtgggtgg gtttcctgga aatagccgct gccaatttcg
    28621 ctggagggaa tatgagcggc aatcgccagt accggaacgt gattgcggtg gcaatcaaac
    28681 aggccgttga ttaagtgcag gttgccgggg ccgcacgatc cggcgcagac cgccagttct
    28741 ccgctaagtt gtgcttcagc gccagcggca aaggccgcca cttcttcgtg gcgggtggac
    28801 atccactcga tggtgcccat gcgattaaga ctgtcactaa gaccattcag agagtcgcct
    28861 gtgactcccc agatgcgttt cacccctgcc gattcgagtg ttttggcgat ataagctgca
    28921 accgtttgtt tcatggttct ccatctcctg aatgtgataa cggtaacaag tttagttcat
    28981 ctgacggagg gggaagggat gggagagaaa ggaggcacta acggttaaat agcccgatga
    29041 aaggaatatc atcgggcata aggcgattat gcgagaacca aatccccctg cggatggcag
    29101 gagcaggcca gtacgtaacc ttcagcgatt tcggcgtcgg tcagcgtcat tgtactgctc
    29161 accgtatatt caccggaaac cacttttgtc ttacagcagc cgcaaacacc cgcacggcag
    29221 gcagcgacaa ccggaacgtt attgctttcc agcgcctcca gtagcgtggt gccaaccggg
    29281 gcgtaaaatt ctcgtgccgg ttgcagtttg gtgaatttca gaccgctggt cgccgctttt
    29341 gctactgggg tgaagaattt ctctttaaag aaacgcgtca cgccgagcgc tttcacttcc
    29401 tgctctaccc aatccatata cggagccggg ccgcaggtca tcacggtacg tgaagctaag
    29461 tcaggtacac ctgccagcag ttctcgagtg agacgaccag cgataaagcc ttcggtaacg
    29521 ttattttctg ccaccagcgt taccggatag ttacgccact catcggcgaa aataacatcc
    29581 tgcggcgtat gcacgttgta gatcacccgc acatcggcct gtggacggtt cttcgcaagc
    29641 cagcgacgca tcgacataat cggcgtgacg ccgcagcctg ccgccagcaa caggaattta
    29701 tcttctgctt tatcgtcgca ggtaaattcc cccatcgcgt ccgaaagcca gagataatca
    29761 ccgcgtttta catcgcgcgt cagccactgg gagccgacac cgtcatcaat ccgccgcacg
    29821 gtcagggtga tatattcact cacgcctggc gtggaggaaa tggtgtaagc acgcagcgtt
    29881 tccgctgagt ttcgcacgct gaccagtgca tattgcccgg cgcgatatgg gtagtaatcg
    29941 tggcaaatca gggaaatcgt ccacacatcc ggcgtttctt gcgtaatgtg atgaacctgc
    30001 atccgccacg ggcattgatt cgttggcatc gtcatcgaca aactccttac gcgctcaaca
    30061 gttgcttcat gtcttcttca acagtggtga tagaacgcag gccgaatttc tcgttcagca
    30121 ccgccagcag gtctggtgtc aggaaaccag gtgcagtcgg gccggtgacg atatttttca
    30181 cgcccagaga aagcagcgtc agcagaatga cgatcgcttt ctgttcaaac caggagagca
    30241 ccagcgacag cggcagatcg ttcacaccac agcccagttt atctgccaga gtgacagcca
    30301 gaataatcgc tgagtaagca tcgttacatt gacctgcatc taccagacgc ggcagacctt
    30361 cgatatcgcc aaactcaagt ttgttaaagc gatatttacc acaggcgagg gtcaggatca
    30421 ggcagtcatc cggcacgatg gtggcgaaat cggtgaagta gtggcgctcg ccgcgtgcgc
    30481 cgtcacagcc accaagcagg aagatatgac gcagtttttc acggctcacc agatcaatca
    30541 gcgtatcagc agcgccaagc agcgtctggc gaccaaaacc cacggtgata aggtgcggaa
    30601 tttcgctgta cgggaagcct gccatctgtt gcgcctgggt gataaccgca gagaaatcat
    30661 caccgtccag atgacgcacg ccaggccagc caacaatgct gcgggtccag atacgatcgt
    30721 cataagcgct tacggttggg tcgatgatgc agttcgaggt catcacgatg gggccaggga
    30781 aacgagcgaa ctccacttgc tgattctgcc agccgctgcc gtagttaccg accagatgct
    30841 tgaatttacg cagctccggg tagccatgcg caggcagcat ttcgccgtgg gtgtagacat
    30901 taacgcccgt gccttcggtc tgttccagca ggttgtagag atctttgaga tcgtgaccgg
    30961 aaatcagaat gcatttaccc gccgtcgctt tgacgttgac ctgggttggc gtcgggtgac
    31021 cgtatttacc ggtttcgcct gcatccagaa tgctcatcac tttgaagttc atctggccga
    31081 tttccattga acactcaaga agcgcgttca tatcggcagg ccaggtcccc agccacgcca
    31141 tgattttatg gtactgggca taaatatcgt tgtcgtattg accgagaaca tgcgcgtgtt
    31201 ccatataggc cgccgcacct ttcaggccat acaggcacag cagacgcagg ccgagaatgt
    31261 tttcgccaat cgccgcttta tctttgttag gggtaaattc tgctgcctga cgttgcagct
    31321 cgccgagatc atcgctcacc agttgcaggt cagccatcgg gttatcgacg cgcgcgttgg
    31381 catctacagc caggcattgt gctttcagcg cctcgcgcaa ggcaatcgct tcacgagcgt
    31441 agccgacaat acgcggagaa tcgaagttaa cgttggtcag ggttgagaaa aaggcacgtg
    31501 gcgcgaagct gtcaacatcg tggttgatga tgccgtattc acgcgctttt accgcccagg
    31561 cagaaagccc ttgcagcgcc gcgatgagta aatcctgaag gtcagaagtt tccgccgttt
    31621 taccacacat cccctgcgcg tatgagcagc cgtttcctgc cggagtacgg atagtttgtt
    31681 cacattgcac acaaaacatg atcacacctt ttaaagttat atttaatata catgtttaag
    31741 gttaagacgc ttaacgcggg gataaaaggg atttttcatg caactttaag ggagattgat
    31801 ttagcgcaat tttggcggca gggatctacc gccagagagg tattacgcag agaaaaaggc
    31861 gatgaggatc ggcactaaca ggctaagaat aaaaccgtga acaattgccg ccgggaccat
    31921 atccagcccg ccagtacgtt gaagaacggg cagggtgaaa tccattgatg tggcaccgca
    31981 taagcccagt gcagtagagc ggctgcggcg aatcagccca gggatcaaca taatagcaat
    32041 cagttcacgg gccagatcat taaaaaacgc cgcgctcccg attaccggac caaaagattc
    32101 ggtcaataaa ataccggaaa gagaatacca gccgaaaccg gaggccattg ccagcgcggt
    32161 attgatgggg agatcaagaa taaaggcgtt aattaaacca ccaattaatg aactgacaac
    32221 caccaccacg gcgacaatca ttccccggcg attaaggaca atctgcttta aggtcatgcc
    32281 attattgcgc aactgaatac caacgaggaa aagtagcaaa attaacgtgt attcactggc
    32341 ttcggtcgcg tgttgtaaga aagccagtcc actcagacca atggcaaaac caatcactac
    32401 tacgccgcac agttttagcg actccagcgc catcgcaata cgcgacggga gtttttcttg
    32461 ctgatggtgg ttgcgccacg gcaggcctcg ctccagccac atcagggcgg caatattaca
    32521 cagtaaaata acggtaatac tgacggcaga ataatgcaga atcgccaaca ggttactggc
    32581 gaggttatcg agaaacgcca gactgatacc cataaaaaag agaataaggt aaaccatcca
    32641 gcttaatagc tgattaataa cttttaacgc agcttgttgg cgaagcggaa tgaggtaacc
    32701 cacaatcagg ggaaccagaa tgattaacag cccagaaaac atgaaaaccc agtccttgca
    32761 aagatgaagt cgaaatgcgc gatgacacac tactgaaagc ggaaggacga gtaaagttgc
    32821 aattaaaagg aaatgttatg cataaggagc agtagagtat tcgttttcat ttaaagatat
    32881 tcttgcgctt taattacaaa ctgcaccgat gttggtggcg tcaaaatcgc cgaggcgttc
    32941 cctgaaggcc ggggcagccc acatggatgt gggctgaggg cgcgttttac agggatgtta
    33001 cctcgcgccc gacccggtag ccgtaaggga taagtcgagg gcaccgcgca gcggcgattt
    33061 tgttcgccag agcccggggg ggcagggggc ggcggcgatt ggccgccccc tgcgcgctcc
    33121 ttgcgccagt ggcaatatgt ggcttagctc atgaaaggag cgcaacaaga tgatgaatca
    33181 acatataaca acatcttaaa aaaagcctga cattacgcca ggccttctgc gttaattaat
    33241 cacgcttttc cagcagggtc cggtaaatca gaccaccgat aatgccgccg acaattggca
    33301 ccacccagaa gaaccacagt tgttctaatg cccagccgcc ctggaagata gcaaccgcgg
    33361 tgctgcgcgc cgggttaaca gaagtgttag tcaccggaat actaattaag tgaatcaggg
    33421 ttaaggccag accaatagcg atcggcgcaa aacctgccgg cgcgaatttg gcggttgcgc
    33481 cgtggatcac caacaggaaa cctgcactca ataccagttc aactaccagc gcggaaagca
    33541 tggaataacc gcctggtgaa tgctcgccat aaccgttaga agcaaaaccg ctggctgccg
    33601 cgtcaaaacc cgttttacca ctggcaatta aatacagcag cgccgctgca acaataccgc
    33661 cgacaacctg ggcaattacg tagccaacga cttcttttgc cggaaaacgt ccgccagccc
    33721 ataaaccaat agtgaccgcc gggttaaaat gaccaccaga aatatgacca acagcaaagg
    33781 ccatcgtcag aacggtcaga ccgaacgcca acgccacgcc ggcaaaacca atgcctaatt
    33841 ccgggaagcc tgcggccagt acagcactac cacagccacc aaaaacaagc cagaaagtac
    33901 caaaacattc agctgctaat tttctgaaca tatccacctc aattaaaaat tgaccctgtg
    33961 aaaaatatgg tcgttttata gggtcgtcgt aaaaaaatga cgacggaaat aatgcgcggc
    34021 tattttaaaa acgaaggcga gtcattcacc agataaataa atccagtaaa tttgatttag
    34081 ggcaacagcg ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa
    34141 gatttaatcc tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg
    34201 tttttgcaga cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca
    34261 tttgccatgc ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc
    34321 agcgtaaagg ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt
    34381 gtgggttttc gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt
    34441 ggggagaacg cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca
    34501 gaatcagatc tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac
    34561 gggcgagaac atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat
    34621 cgggttcgcc gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac
    34681 cgtatttttt atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc
    34741 agttttctcg ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc
    34801 catctggtgc gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt
    34861 cgtaacggca cggtgccaaa tgtccctaat gtggaagtca ccgcgcgcca gctcgatttc
    34921 ctcgcccgtg agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga
    34981 ctttccgcaa tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg
    35041 cgatatcgtt taatgcggcg gcgggccagc aatgagcaac gaagctggcg ctatctggac
    35101 atcatcaacc ggatgattga ccgacctgga gggcgctcgt atcgggttat tttgctcggc
    35161 ctgtttgcta ctttattgca ggcaaaaggc acattgagac tggataaaga cgcgcgtcca
    35221 ttgttgctga tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg
    35281 catctgttga atcttctgcc attgcagcgc attgccacca ccaactcggg tgagttgctt
    35341 tcgttaacgc cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc
    35401 tggcgtctgg ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt
    35461 cgttttaacc gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg
    35521 gaaacctggg ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg
    35581 atcaaggtca ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga
    35641 atggggattg aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct
    35701 acggtacgca gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta
    35761 ccggcgctgg atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc
    35821 gtggcgcaaa tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc
    35881 catcgctctt ccaaacccga tcttgccatt gaagtggcaa tagaggcagg acgtcgtggt
    35941 gtggactccg taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc
    36001 ggtcgcgcgg attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc
    36061 tcatagcgcc gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt
    36121 gctatctgtg ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc
    36181 cagaaagcgt tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg
    36241 taaatatgcg tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac
    36301 agacaggccg cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca
    36361 ttctggattt cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc
    36421 gttcttttcc cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg
    36481 ctgttgcgaa acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc
    36541 ttaagcgtga actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta
    36601 ttcaaatata agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa
    36661 cgtaacgcct ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga
    36721 cgcacaggca gtcgcggctg gcgcgttaac aacacattga gattaggcca gtgggaaagt
    36781 tcgttcatcc actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg
    36841 cgaaaactac ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga
    36901 aaaagtgaca ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac
    36961 atattcatga aatcattggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa
    37021 acatcaatag tgtgattact aacgtaaatt ttagggtttt gttgatattt cgttgaagtt
    37081 aatgacccgg attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga
    37141 agaagcgtta cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa
    37201 ttcttaacgc acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc
    37261 aaagcgtgct ggcgaccgga aagctggacg cgctgcgtaa ggttgatgtg ggcgcgcagg
    37321 tcagcggtca gttgaaaact ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc
    37381 ttttaggggt tattgatcct gaacaggctg aaaaccagat taaggaggtc gaagcaacgc
    37441 tgatggagct acgtgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga
    37501 cgtattcccg tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca
    37561 ccgccgcgac ggagatggct gtgaaacagg cgcaaattgg caccattgac gcggaaatca
    37621 agcgcaatca ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg
    37681 ccccgatggc cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg
    37741 cacaacaagc accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc
    37801 aggtttctga agcggatgta atccacctga agccggggca aaaagcttgg tttacggtgc
    37861 ttggcgatcc actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa
    37921 aggttaacga cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc
    37981 tgcggctgga tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga
    38041 cgatccctct gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt
    38101 tgcgtaatgg tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg
    38161 ttgagattgt caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag
    38221 gagctgcaca atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg
    38281 tgatgagcag gttgaggtgc tgaagggcat caccctcgat atttatgccg gtgagatggt
    38341 agcgattgtt ggcgcttcag gttccggtaa atcgaccctg atgaatattc tcggctgtct
    38401 ggataaggcc accagcggca cctatcgcgt cgccggtcag gatgttgcca cactggacgc
    38461 cgatgcgctg gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt
    38521 actttcgcat ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ccggtcttga
    38581 gcggaaacag cggctgcttc gcgcccagga gttactgcaa cgactggggc tggaagaccg
    38641 tacagagtat tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg
    38701 ggcattgatg aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag
    38761 ccattctggc gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatatggt
    38821 gattatcgtc acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg
    38881 cgacggcgaa attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac
    38941 ggagcctgtt gtcaacacgg tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc
    39001 gctgacgatg gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct
    39061 ggggattatt atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa
    39121 acaaatggtg ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg
    39181 gaaagatttt ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat
    39241 cgccatccaa aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg
    39301 cctgcgttat aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt
    39361 taatgtctat ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg
    39421 tcgtgcgcag gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc
    39481 agatgtggtt ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc
    39541 ggaagaaaaa cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag
    39601 cacgatgtcc gggcgagtta tgggccagtc gtggctgaac tccattactg tcagggtaaa
    39661 agaaggattt gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca
    39721 cggaaagaag gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac
    39781 cacacgtact ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggcgg
    39841 tattggtgta atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat
    39901 tcgcatggct gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt
    39961 actggtttgc ttggtcggtg gcgcgttggg aataacgctg tcactgttaa ttgctttcac
    40021 cttgcagctt ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc
    40081 gtttctctgc tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc
    40141 acgactggat ccagtagatg ctctggtacg agagtaattt ttgagataaa aatgccagcc
    40201 gatcgggctg gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg
    40261 ccgcttctac ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga
    40321 catcaaactg aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg
    40381 aataatgagc gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg
    40441 cattgttgaa ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat
    40501 acaagtaaga tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct
    40561 agaaatacaa tttagagaat tagggcgagc cgtcaagcgt ttgacagggg ccagggggca
    40621 ggtatgaatc aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct
    40681 gatgccaacg atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc
    40741 tcttgtcagc ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg
    40801 gtaaaacgaa cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc
    40861 taaaaccgcc atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt
    40921 ttgttattga cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga
    40981 tgctcgctgt tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag
    41041 aaaccaaagt ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta
    41101 cgctagaaaa agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag
    41161 aactggaact cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta
    41221 tgaccgtcga gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg
    41281 aagcgtgttc tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa
    41341 ccacacccgt tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc
    41401 agcgtgtact gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggtaaccg
    41461 gtgcaaacgt tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc
    41521 gtaaacatga agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag
    41581 acgagccgac acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg
    41641 gggaggaacg tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa
    41701 tcgacccact gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc
    41761 gccgtaaaaa caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg
    41821 aaggtcttgc ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga
    41881 tttactctct cgatatcggt tctctgttag cgggcactaa atatcgcggc gactttgaaa
    41941 aacgttttaa agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg
    42001 atgagatcca caccattatc ggtgcgggtg cagcgtctgg tggccaggtc gatgcggcta
    42061 acctgatcaa accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc
    42121 aggagttcag caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg
    42181 atattactga accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt
    42241 atgaagcgca ccacgacgtg cgttataccg caaaagcggt gcgtgcagcg gtagagctgg
    42301 cggtgaaata cattaacgat cgtcatctgc cggataaagc cattgacgtt atcgacgaag
    42361 cgggcgctcg cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg
    42421 atattgagtc cgtggtggcc cgtattgcgc gcattccaga gaagagtgtt tctcagagtg
    42481 accgcgatac cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata
    42541 aagccattga ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac
    42601 ataaaccggt tggttcgttc ctgtttgccg gccctaccgg ggtcgggaaa acagaggtga
    42661 cggtacagct ttcgaaagcg ttgggcattg agctgctgcg ctttgatatg tccgagtata
    42721 tggaacgcca taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc
    42781 agggaggttt gctgactgat gcggtcatca agcatccaca tgcggtgctg ttgctggacg
    42841 aaatcgagaa agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta
    42901 cgctgaccga taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca
    42961 ccaacgccgg ggtacgtgaa actgagcgta aatccattgg tcttatccac caggataaca
    43021 gcaccgatgc gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg
    43081 acaacattat ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg gtggataaat
    43141 tcatcgtcga gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg
    43201 aagcgcgtaa ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg
    43261 cgcgtgtcat ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc
    43321 tggtggacgg cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg
    43381 gattccagag tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgttaggtag
    43441 gttggtcaag tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaggag
    43501 gttcagaccc tttttttaat gatgatggta agttgttgat aattagtgct gcgggaaggt
    43561 aaggataaaa aagggtgctg caggagaatg ggatggtttt gctttattta caacgggcta
    43621 aacgtgtagt atttgagttc actgccgtac aggcagctta gaaattcaca ggtaacatac
    43681 tccacccgcc caccatgttc actgccgtac agacagataa aatgcgaaaa aaaagctcgc
    43741 actttcgtac gagctcttct ttaaatatgg cggtgagggg gggattcgaa cccccgatac
    43801 gttgccgtat acacactttc caggcgtgct ccttcagcca ctcggacacc tcaccaaatt
    43861 gttttgctac caaacctcat gggtggcaac ggggcgctac tatagggagt tggagtaaaa
    43921 cggtcaagaa gaattttaat gataattatt gtttgctcat actgtaaaca agttgtgcag
    43981 tatatctaca tcgagacaag ttacggactt atacttccaa agtacttcat acatatcaca
    44041 aaataaaaag gccggttaaa ccgacctttt actcgttctt tctcttcgcc catcaggcgg
    44101 taaaacaatc agcgactacg gaagacaatg cggcctttgc tcaggtcgta cggggtcagt
    44161 tcaacagtca ctttgtcgcc cgtcaggatg cggatgtagt ttttgcgcat tttaccggag
    44221 atgtgtgcag taaccacgtg accgttttct aactctacgc ggaacatggt attaggcaac
    44281 gtttcaagaa cggtaccttg catttcaata ttgtcttctt tggccatcta atcctctggg
    44341 gtatcactac cgtaatttga accggcaaga taatgccgaa gttctgtaaa taagtaaaga
    44401 tttgcgcgct aaatcgcaac aaacaggttc ggcacattac tccgaaaaca cacggctaag
    44461 ccgcaccaaa agcgcaacgt ataagggagc ggtgagataa acgatgggcg ttacctgacg
    44521 cgaaaaattc cttatcggca gcggggtaat gagcgtaacc aactctgcga ccgcaattat
    44581 aacactctgg ggagaaatgt gccgaaaaca ttcattcttg tggtgaaaac aagcatcgtg
    44641 gtacccagaa attattcggc aatcgtccga ggcgcatttg attgagataa ttaaggtaat
    44701 cccggcgggg aatttcgcag gcaccaagcg atgctgtgtg atcgttaagg acctggcagt
    44761 cgataagctt accgccatga ccgataaatt cctcacagaa taccagaagc gccgttttag
    44821 acgcattttc catccggctg aacatggact cgccacaaaa tagcgttccc tgggccacgc
    44881 cgtacatacc gccgacaagc tcatcttcac gccagacttc aatggagtgg gcatggccga
    44941 gttcgtgaag gcgatggtag gcttcgacca cgccacgcgt gatccaggtt ccttcttcgc
    45001 gatcgctggc acagccttca atgacctgac caaaagcgta attcatcgtg acacgatagg
    45061 gcgagcgttt atgaaatcgc ttcatactac ggctgatatg cagtgattct ggccatagca
    45121 ccgcgcgggg atcgggcgac caccagagga tggggtcgcc tggagaaaac cacggaaaaa
    45181 taccacgctg gtaagccatt aacaggcgcg cagggctaag atcgccccca agtgccagca
    45241 ggccgttagg ctcacgtaat gcgccttccg gggaagggaa ggctattgaa tggcgagaaa
    45301 gctgaaccag gcgcatgacc gcaaaactcc acgcaagtcg gatcgttcaa taatagctta
    45361 caaaccctgc ttgaactggt aataacgccc ctgtctggca agcagctctg cgtgagtacc
    45421 ttgctcaata atttgcccgt tgtccatcac tattatttgt tggaaacgag agagtccgcg
    45481 aagtcgatgg gtgaccatta acaccgtttt ctcacgcatc atttctgcaa gcaattcaag
    45541 gatctggctt tcggttgtgg catctaagcc ttcggtaggt tcatccagca acaccagtgg
    45601 cgcatcatgt aacagcgcac gggcgatagc cagacggcgc agttcaccac cggagagctg
    45661 gcgtccgcct tcacctaacc aactgttgag acctgcatcc tcgagcagct tctccagacc
    45721 aacgcgacgc aagctctccg ccagagcctc atcactactg ccaggcgagg cgagtaacag
    45781 attatcacgc agcgtggcgc taaacagatg cactcgctga ggaacaacgc tgatggtctg
    45841 tcgtagagcc gcttcattca ggctggctat ggggctatcg ttaagcaaaa tctcgccctg
    45901 ttgcgggtcc caagcgcggg tcagcagttg taacagtgtt gatttgccgc atccggttcg
    45961 cccgagaatc gcaatatgtt ccccggcgtt tacctgaaga gaaatccctt taagtgcctg
    46021 ttgggattgc tccggataag tgaactgaac atcccgtaat gtcagcgaaa cgcgatcggc
    46081 aacacgagtt tgggtatcag ggaaggtgac ttccggtttt tgatccgtta aatcagtgat
    46141 ccgaacggca gaggcaatga cttgccccag atgctgaaat gcacccgtta ctggtgccag
    46201 tgcttcaaac gcggctaacg cgcagaagac aaacagggca attaacgcgc cgggttgagc
    46261 attgccgcca acgccgccag acgccatcca cagcatcagg atcaccgcta acgcgccaat
    46321 gagcagcatt atcgcttgcg acaatgcggt cagttcagat tgacggcgtt gcgcttccag
    46381 ccattgaatt tctgtattct ctagttgcgt gcgataacga tcgctggcac caaaaatggt
    46441 cagctcagct tgcccttgca gccaggccgt cagttgttgg cgatactgtc cgcgaagatg
    46501 agtcagattt tgcccggtgc tttttcccgc acgataaaac agcggtggca tcaggaaaag
    46561 cgtcagtaac ataatgccgc ccagcgtaaa ggcgagggtg aaatcaagga aacttaaccc
    46621 gattgtcacc atcataatca ccacaaaagc gcccaccagc ggcgagataa cgcgcaggta
    46681 aagatgatcg agcgtatcaa catccgccac cacgcgattg agcaattcgc cctgacgata
    46741 gcgcgccagt ccggcagggg agaggggcaa caatttgctg aaggtgtaaa tgcgcagatg
    46801 ctgcaacacg cggaaagtcg cgtcgtgact taccagacgt tcaaaataac gcccggcagt
    46861 acgggtgatt gcggcaccac gcacgcccgc agcgggtagc atatagttga agctgtacag
    46921 tccggcaacc cccgcaaccg ctgaggccga gaggaaccag ccggaaagtg tcaacagtcc
    46981 gatactggcg agcagcgtca caattgccag cacaatacca agacttaaca tccatttatg
    47041 acgtttatac agtgctagat agggtagcaa agcgcgcatt taaatctcct cctgacgatg
    47101 ggccagtaat gtggcgaatg ggccaccagc cacacttaat tccgcgtaac gtccttgctc
    47161 aataatccgg ccatcctgca taacccaaat gacatcccag tcagcaagat cttctaactg
    47221 gtgggtgacc attaacgttg tctggcgcag agaggcggca ttcagcgcct ccattacgcg
    47281 ctgttcactg tgagcatcaa ggctggcagc gggttcatcc aacagtaata gcgaacaggg
    47341 atttagtaac gcacgggcca ccgccacgcg ctgcgcctgc cccacggaaa ggcgggcagc
    47401 ctggtcgcca acaggcgtat caacgccttg tggcaggagc ggtagaaact cgctgaccca
    47461 ggcgttatcc agcgctgctt gtaattcatg ttcgctggca tcaggtcgcg ccagtagtac
    47521 gttatcccgt aatgttgctg ccggtaattg tggattttgc ccaacccagg agagatgttt
    47581 acgccatgat tctggtgata aatcgcgtaa ttctatcccg ttgattcgta gcgatccctg
    47641 atatgagaga aaaccagaaa gcgcgttcaa cagtgaactt ttacctgaac cgctgcgacc
    47701 aaccaacacg gcacgttggc ctgctggcaa agtaaagttc agcggtccgg ccagcgtttt
    47761 accttccggc gacgtgataa acagatcctc ggcctcaatg gtcaccggat cggtcgatgc
    47821 taattccacc tcgccacact gcggatgggc gagcggggtt tccataaacg ttttcagact
    47881 gtcagctgca ccaacagcct gggctttagc atgataaaac gtaccgagat cgcgtaatgg
    47941 ctggaaaaac tctggcgcaa ggatcagggc cagaaaaccc gcagccagcg tcacgccagt
    48001 atcgtagtga ccaaaatcca gctcgccaag ataggaaaaa ccaaagtaga ccgccaccag
    48061 agcaattgac agcgaggtaa aaaattcgag aatgccggag gataaaaatg ccagccgtag
    48121 cacttccatt gtccgttggc ggaaatcttc cgaagcagaa cgaatacttt caatttcagc
    48181 ttcaccacga ccaaaaatac gcaatgtttc catgccgcgc aggcgatcga ggaaatgccc
    48241 acttaagcga gcaagagcga gaaagttacg tcggttagca tcggcagccc ccattccaac
    48301 cagcgccata aacaacggaa ttaacggtgc agtgcccagc agaatgagcg ccgcagccca
    48361 gtttgagggg aagattgcca ccacaatcag caacggcacc gacactgcca gcgccatttg
    48421 cggcaggtag cgtgcatagt aatcatgcat atcgtcaatt tgctcgagta ccagcgtcgc
    48481 ccagctcccc gcaggtttac cctgaatcca cgctggccct gcttgttgca gacggtcgag
    48541 aacctgccgg cggatggcaa agcggatatg ctgcccggcg tgataaccca cccgttcgcg
    48601 taaccagacc acccatgcgc gcagtacaaa ggtcagaacc agtaacgtaa agggaagcag
    48661 cagggcttca cggggaatat tctccataat catatgttgc agaattcgcg ccatgaacca
    48721 ggcctgggca atgatcaata tgccgctcac aaagcccagc agacgagaaa tattcagcca
    48781 acgttgggag atgacgcttt gctgttttaa ccagcgggtt aactcttttt gacgagattt
    48841 attcattgca cgcttagcag gtgagttatc agaattattt gcagagcaat gttacaacgg
    48901 ggaaaaaata aaggcgaccc atagtcgcaa ggtgtcgcct tctttacttt tgttactgat
    48961 ttgtaaaatt attttgcgtc agctaaacca tcgaggtagc gttccgcatc aagtgctgcc
    49021 atgcagcctg taccggccga agtaatggcc tgacgataaa tatgatccat cacgtcaccc
    49081 gcggcaaaga cgccaggaat gctggtctgg gtggcattac catgaatacc cgactgtact
    49141 ttgatgtagc cgttttccag ttccagctgc ccttcgaaaa tggcagtatt cgggctgtga
    49201 ccgatagcaa caaacagacc ggcaacgtcg agtgattcga tgttatcgct gttttgcgta
    49261 tcgcgcagac gaacgccagt gacacccatt tgatcgccgg tcacttcttc cagcgtccgg
    49321 ttggtgtgca gaatgatgtt gccgttctcc actttatcca tcagacgctt aatgaggatt
    49381 ttttccgcgc ggaaaccgtc acggcggtga atcagatgta cttccgaagc gatgttagac
    49441 agatacaacg cctcttcaac cgcggtattg ccgccgccga tgaccgcaac tttctggttg
    49501 cgatagaaga aaccgtcgca ggttgcacaa gcagaaaccc cacggccttt aaaggcttct
    49561 tcagagggca ggccgagata gcgtgcagaa gctccggtgg caataatcag cgcgtcgcaa
    49621 gtgtattcgc cgttatcgcc attcagacgg aacggacggt tttgcagatc caccttgttg
    49681 atatgatcaa aaatgatttc agtttcaaat ttggtggcat gttcgtgcat gcgctccatt
    49741 aataacggac cggtcagatc gtttggatcg ccaggccagt tttccacttc tgtggtggtg
    49801 gtcagttggc cgcctttttc catgccggta atcagcacag gttgcaggtt ggcgcgcgcc
    49861 gcatagacag cagcggtgta tcccgccggg cctgaaccca ggataagcag tttactgtgt
    49921 ttggtcgtgc ccatgagatc cccatagttg ttggcagaca atgggcagga ttgtagggaa
    49981 tttactgacg taaaaaaaga gtatgacgat tttgttaaca atttgtgcaa tcggcagcat
    50041 cgataagcag gtcaaattct cccgtcatta tcacctctgc tacttaaatt tcccgcttta
    50101 taagctgatt aaatgatgaa taaacgcccc tgttaatgaa tatctggcat gttgtactaa
    50161 aaatcgatgt tttgctttga caatcccctg gtgttttgcg aaaacattcg aggaagaaaa
    50221 aaaacagtat tcttatatgc gcataaccat gcatgtaaat accatgttta ccgtgctagt
    50281 gaaatctacg tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa
    50341 cggtcttctc accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat
    50401 cgtgacgggt agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa
    50461 tacagagaga caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt
    50521 atcgatcgta acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt
    50581 tctaaacgtg tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga
    50641 caagggttta ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt
    50701 ctggtattcg ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat
    50761 accgctgtac aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac
    50821 tacctgttga aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc
    50881 ctgctgcgtc tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag
    50941 cagagtaatc gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat
    51001 tttgattaca ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt
    51061 ccgttttagc atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat
    51121 acactgaaga caaagaagtc acattgacaa agttaagtag cggccgtcgc cttctggaag
    51181 cgttgctgat ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta
    51241 acccttcgga ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg
    51301 ggatgcccgg tgcgtggctg gcagatacgc tgttctttat ttttggcgtg atggcttaca
    51361 ccattcccgt cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagtgacg
    51421 aatacattga ttattttgcc gtttcgttac gcatcattgg cgttttggcg ctcatcctta
    51481 cctcctgtgg tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg
    51541 tcattggcag cttactaagc actacgttac aaccactgct acacagtagc gggggaacta
    51601 ttgcgctgct ctgcgtttgg gctgcgggcc tgacgctgtt caccggttgg tcatgggtga
    51661 ccattgctga aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta
    51721 cccgtcgcga tgatacctgg gtcgatgaag atgaatatga agatgacgaa gagtatgaag
    51781 atgaaaacca cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag
    51841 cgcgtcgtaa acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg
    51901 cgttgttctc cggtaagcgg atggatgatg aagaagagat tatctacact gcacgcggtg
    51961 tggctgccga cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg
    52021 acgaatacga tccattatta aacggtgcgc caattaccga acctgttgct gtagcagctg
    52081 ctgctaccac ggcgacacaa agctgggctg cgccggttga acctgtgacc cagacgccgc
    52141 ctgttacttc tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg
    52201 gtccacaaac gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagccac
    52261 aatatgcgca gcctgcagtg caatataatg agccgctgca acaacctgta cagccacagc
    52321 agccgtatta tgcgcctgca gctgaacaac ctgtgcaaca accttactac gctcctgcgg
    52381 cggaacaacc tgttcagcaa ccgtattacg caactgcgcc ggaacagcct gcgcaacagc
    52441 cgtattatgc ccctgcgcca gaacaaccgg tggcaggtaa cgcctggcaa gccgaagagc
    52501 agcaatccac ttttgctcca cagtctacat accagactga gcaaacttat cagcagccag
    52561 ccgctcagga gccgttgtac caacagccgc aacccgttga acagcagcct gttgtggagc
    52621 ctgaacccgt tgtagaagag acaaaacccg cgcgtccgcc gctttactat tttgaagaag
    52681 tggaagagaa gcgagcccgt gaacgtgaac aacttgcggc ctggtatcaa ccgattccag
    52741 aaccggttaa agaaccagaa ccgatcaaat cttcgttgaa agcgccttct gctgcagcag
    52801 tacctccagt agaagccgct gccgctgttt ctccgctggc atctggcgtg aaaaaagcga
    52861 cactggcgac gggggctgcc gcaaccgttg ccgcgccagt cttcagtctg gcaaatagcg
    52921 gtggaccgcg tccgcaagtc aaagagggaa ttggcccgca gttgccacga ccgaaacgta
    52981 ttcgcgtgcc aactcgtcgt gaactggcgt cttacggtat taagctgccc tcacagcgtg
    53041 cggcggaaga gaaagcccgt gaagcccggc gcaatcagta tgattctggc gatcagtata
    53101 acgatgatga aatcgatgcg atgcagcagg atgaactggc acgtcagttc gcccagacac
    53161 agcagcaacg ctatggcgaa cagtatcaac atgatgtgcc cgtaaacgca gaagatgcag
    53221 atgctgcggc agaggctgaa ctggctcgtc agtttgcgca aactcaacaa caacgttatt
    53281 ccggcgaaca accggctggg gcgaatccgt tctcgctgga tgattttgaa ttttcgccaa
    53341 tgaaagcgtt gctggatgat ggtccacacg aaccgttgtt tacgccaatt gttgaacctg
    53401 tacagcagcc gcaacaactg gttgcaccgc agcagcagta tcagcagcca caacagcagg
    53461 ttgcgccgca gccacaatat cagcagccgc aacaaccggt tgcaccgcag cagcaggata
    53521 ccctgcttca tccgctgttg atgcgtaatg gcgacagccg tccgttgcat aaaccgacga
    53581 cgccgctgcc ttctctggat ttgctgacac cgccgccgag cgaagtggag ccggtagata
    53641 cctttgcgct tgaacaaatg gctcgcctgg tggaagcgcg tctggctgat ttccgtatta
    53701 aagccgatgt cgtcaattac tctccggggc cggttatcac tcgctttgaa cttaacctgg
    53761 ctcctggcgt aaaagcggcg cgcatttcta acttgtcacg ggaccttgcc cgttcacttt
    53821 cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa accctatgta ggtctggagt
    53881 taccaaataa aaaacgacaa accgtttatc tgcgcgaagt tttggataac gccaaattcc
    53941 gcgataatcc gtcgccatta accgtggtgc tgggtaaaga tatcgccggt gagccggtgg
    54001 ttgccgatct ggcgaaaatg ccgcacttgt tggttgcggg gactaccggt tccggtaaat
    54061 ctgtcggtgt gaacgcgatg atcctgagca tgctttataa agcacagcca gaagatgtgc
    54121 gtttcatcat gatcgacccg aaaatgctgg agctttcggt ttatgaaggc attccgcatc
    54181 tgttaacgga agtcgttact gatatgaaag atgccgccaa cgcgctgcgc tggtgtgtta
    54241 acgagatgga gcgtcgttat aaactgatgt ctgcgctggg tgtgcgtaat ctggcgggtt
    54301 ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc gattccagac ccgtactgga
    54361 agccgggtga cagtatggat gcccagcatc cggtgctgaa aaaagaacca tacattgtgg
    54421 tgttggttga cgaatttgcc gacctgatga tgacggtagg taaaaaagtg gaagagctga
    54481 tagcacgtct ggcgcaaaaa gcccgtgccg cgggtatcca cctcgtactg gcaactcagc
    54541 gtccatcggt tgatgttatt actggtctga ttaaagcgaa tattccgacc cgtatcgcct
    54601 ttaccgtatc cagtaagatt gactcacgta ccattcttga tcaggctggc gcggaatcac
    54661 tgctggggat gggggatatg ctctactctg ggccgaactc cacgttgccg gtacgtgtcc
    54721 atggtgcttt tgttcgcgat caggaagttc atgccgtggt gcaggactgg aaagcgcgtg
    54781 gtcgcccaca gtatgttgat ggcatcactt ccgacagtga aagcgaaggt ggcgcgggcg
    54841 gtttcgatgg cgctgaagaa ctggatccgt tgttcgatca ggcggtgcag tttgtcactg
    54901 aaaaacgcaa agcgtcaatt tctggcgtac agcgtcagtt ccgcattggt tataaccgtg
    54961 cagcgcgtat tatcgaacag atggaagcgc aggggattgt cagcgaacag gggcacaacg
    55021 gtaatcgtga agtgctggcc ccaccgccgt ttgactaatt aatgcattgc cggataaggc
    55081 gcggtagcgt cgcatccggc actctatcaa ctgaaaattc agtattttct tttttcctca
    55141 agctgattat tagcctggaa tagagagtag agggaactcc cgatcgggag tgacgtaatt
    55201 tgaggaataa tgatgaaaaa aattgccatc acctgtgcat tactctcaag cttagtagca
    55261 agcagcgttt gggctgatgc cgcaagcgat ctgaaaagcc gcctggataa agtcagcagc
    55321 ttccacgcca gcttcacaca aaaagtgact gacggtagcg gtgcggcggt gcaggaaggt
    55381 cagggcgatc tgtgggtgaa acgtccaaac ttattcaact ggcatatgac acaacctgat
    55441 gaaagcattc tggtttctga cggtaaaaca ctgtggttct ataacccgtt cgttgagcaa
    55501 gctacggcaa cttggctgaa agatgccacc ggtaatacgc cgtttatgct gattgcccgc
    55561 aaccagtcca gcgactggca gcagtacaat atcaaacaga atggcgatga ctttgtcctg
    55621 acgccgaaag ccagcaatgg caatctgaag cagttcacca ttaacgtggg acgtgatggc
    55681 acaatccatc agtttagcgc ggtggagcag gacgatcagc gcagcagtta tcaactgaaa
    55741 tcccagcaaa atggggctgt ggatgcagcg aaatttacct tcaccccgcc gcaaggcgtc
    55801 acggtagatg atcaacgtaa gtagaggcac ctgagtgagc aatctgtcgc tcgatttttc
    55861 ggataatact tttcaacctc tggccgcgcg tatgcggcca gaaaatttag cacagtatat
    55921 cggccagcaa catttgctgg ctgcggggaa gccgttgccg cgcgctatcg aagccgggca
    55981 tctgcattct atgatcctct gggggccacc gggtaccggc aaaacaactc tcgctgaagt
    56041 gattgcccgc tatgcgaacg ctgatgtgga acgtatttct gccgtcacct ctggcgtgaa
    56101 agagattcgc gaggcgatcg agcgcgcccg gcaaaaccgc aatgcaggtc gccgcactat
    56161 tctttttgtt gacgaagttc accgtttcaa caaaagccag caggatgcat ttctgccaca
    56221 tattgaagac ggcaccatca cttttattgg cgcaaccact gaaaacccgt cgtttgagct
    56281 taattcggca ctgctttccc gtgcccgtgt ctatctgttg aaatccctga gtacagagga
    56341 tattgagcaa gtactaactc aggcgatgga agacaaaacc cgtggctatg gtggtcagga
    56401 tattgttctg ccagatgaaa cacgacgcgc cattgctgaa ctggtgaatg gcgacgcgcg
    56461 ccgggcgtta aatacgctgg aaatgatggc ggatatggcc gaagtcgatg atagcggtaa
    56521 gcgggtcctg aagcctgaat tactgaccga aatcgcaggt gaacgtagtg cccgctttga
    56581 taacaaaggt gatcgctttt acgatctcat ttccgcactg cataaatcgg tacgtggtag
    56641 cgcacccgat gcggcgctgt actggtatgc gcgaattatt actgccggtg gcgatccgtt
    56701 atatgttgcg cgtcgctgtc tggcgattgc gtctgaagat gtgggtaatg ctgatccacg
    56761 agcgatgcag gtggcaattg cagcctggga ttgctttact cgcgttggcc cggcggaagg
    56821 tgaacgcgcc attgctcagg cgattgttta cctggcctgc gcgccaaaaa gcaacgctgt
    56881 ctacactgcg tttaaagctg cgctggcgga tgctcgcgaa cgtccggatt atgacgtgcc
    56941 ggttcatttg cgtaatgcgc cgacgaaatt aatgaaggaa atgggctacg ggcaggaata
    57001 tcgttacgct catgatgaag caaacgctta tgctgccggt gaggtttact tcccgcggga
    57061 aatagcacaa acacgctatt atttcccgac aaacaggggc cttgaaggca agattggcga
    57121 aaagctcgcc tggctggctg aacaggatca aaatagcccc ataaaacgct accgttaatg
    57181 ttatcgttgc ggtaatgttg ttactgtatc cctgtggtcg caggctgtgg ccacatctcc
    57241 catttaattc gataagcaca ggataagcat gctcgatccc aatctgctgc gtaatgagcc
    57301 agacgcagtc gctgaaaaac tggcacgccg gggctttaag ctggatgtag ataagctggg
    57361 cgctctcgaa gagcgtcgta aagtattgca ggtcaaaacg gaaaacctgc aagcggagcg
    57421 taactcccga tcgaaatcca ttggccaggc gaaagcgcgc ggggaagata tcgagccttt
    57481 acgtctggaa gtgaacaaac tgggcgaaga gctggatgca gcaaaagccg agctggatgc
    57541 tttacaggct gaaattcgcg atatcgcgct gaccatccct aacctgcctg cagatgaagt
    57601 gccggtaggt aaagacgaaa atgacaacgt tgaagtcagc cgctggggta ccccgcgtga
    57661 gtttgacttt gaagttcgtg accacgtgac gctgggtgaa atgcactctg gcctcgactt
    57721 tgcagctgca gttaagctga ctggttcccg ctttgtggta atgaaagggc agattgctcg
    57781 catgcaccgc gcactgtcgc agtttatgct ggatctgcat accgaacagc atggctacag
    57841 tgagaactat gttccgtacc tggttaacca ggacacgctg tacggtacgg gtcagctgcc
    57901 gaaatttgct ggcgatctgt tccatactcg tccgctggaa gaagaagcag acaccagtaa
    57961 ctatgcgctg atcccaacgg cagaagttcc gctgactaac ctggtgcgcg gtgaaatcat
    58021 cgatgaagat gatctgccaa ttaagatgac cgcccacacc ccatgcttcc gttctgaagc
    58081 cggttcatat ggtcgtgaca cccgtggtct gatccgtatg caccagttcg acaaagttga
    58141 aatggtgcag atcgtgcgcc cggaagactc aatggcggcg ctggaagaga tgactggtca
    58201 tgcagaaaaa gtcctgcagc tgctgggcct gccgtaccgt aaaatcatcc tttgtaccgg
    58261 cgacatgggc tttggcgctt gcaaaaccta tgacctggaa gtatggatcc cggcacagaa
    58321 cacctaccgt gagatctctt cctgctccaa cgtttgggat ttccaggcac gtcgtatgca
    58381 ggcacgttgc cgcagcaagt cggacaagaa aacccgtctg gttcataccc tgaacggttc
    58441 tggtctggct gttggtcgta cgctggttgc agtaatggaa aactatcagc aggctgatgg
    58501 tcgtattgaa gtaccagaag ttctgcgtcc gtatatgaac ggactggaat atattggcta
    58561 atacccaatt tttctgaatc taaaaagcgc ctgcgggcgc tttttttgtc tccctttgat
    58621 accgaacaat aattactcct cacttacacg taatactact ttcgagtgaa aatctaccta
    58681 tctctttgat tttcaaatta ttctatgtat acaagcctat atagcgagct gctatagaaa
    58741 taattacaca atacggtttg ttactggaat caatcgtgag caagcttgag tgagccatta
    58801 tgaaaacgaa aatccctgat gcggtattgg ctgctgaggt gagtcgccgt ggtttggtaa
    58861 aaacgacagc gatcggcggc ctggcaatgg ccagcagcgc attaacatta ccttttagtc
    58921 ggattgcgca cgctgtcgat agcgccattc caacaaaatc agacgaaaag gttatctgga
    58981 gcgcctgtac agttaactgt ggtagtcgct gcccgctacg tatgcacgtc gtggacggtg
    59041 aaatcaaata tgtcgaaacg gacaataccg gcgatgacaa ttacgacggc ctgcactagg
    59101 ttcgcgcctg cctgcgtggg cgttccatgc gtcgccgtgt ctacaatccg gatcgcctga
    59161 aatatccgat gaaacgagtc ggggcgcgcg gtgaaggcaa attcgagcgc attagctggg
    59221 aagaagccta cgacatcatc gcgaccaata tgcagcgcct gatcaaagag tacggcaacg
    59281 agtctatcta tctgaactat ggcaccggta cgctgggcgg caccatgacc cgctcctggc
    59341 cgccgggaaa taccctggtc gcgcggctga tgaactgctg cggcggctat ctgaaccatt
    59401 acggcgacta ctcctccgcg caaattgcgg aaggtttgaa ctatacctac ggcggctggg
    59461 cagatggcaa cagcccgtcg gatatcgaaa acagtaagct ggtagtgctg tttggtaata
    59521 accctggcga aacgcgaatg agtggcagtg gggtgactta ctatcttgaa caggcacgcc
    59581 agaaatctaa tgcccgcatg atcatcatcg atccgcgcta taccgacacc ggtgccgggc
    59641 gcgaagatga gtggatccct attcgtccgg gaacagatgc cgcactggtt aacggtctgg
    59701 cgtacgtcat gatcactgaa aacctggtgg atcaggcatt cctcgataaa tattgcgttg
    59761 gctacgatga gaaaaccctg ccagccagtg cgccgaaaaa tggccactat aaagcttata
    59821 ttctgggtga agggccagat ggcgtggcta aaacaccgga atgggcctca caaatcaccg
    59881 gtgttccggc agacaaaatc atcaaactgg ctcgtgaaat cggcagtacc aaaccggcgt
    59941 ttatcagcca gggatggggc ccgcagcgtc acgctaacgg tgaaatcgca acccgtgcta
    60001 tctcgatgct ggcgattctg accggtaacg ttggtattaa cggaggcaac agcggcgcgc
    60061 gtgaaggttc atacagctta ccgtttgtcc gtatgccgac cttggaaaac ccgatccaga
    60121 ccagcatttc gatgtttatg tggaccgatg ccattgaacg tggcccggaa atgacggcgc
    60181 tgcgtgatgg tgtacgcggg aaagataagc tggatgtgcc gatcaaaatg atctggaact
    60241 atgccggtaa ctgcctgatt aaccagcatt ctgaaatcaa ccgtacccat gaaatccttc
    60301 aggatgataa gaagtgcgag ctgattgtgg ttatcgactg ccacatgacc tcatcggcga
    60361 aatatgctga catcctgctg cctgactgca ccgcttccga acagatggac tttgcgctgg
    60421 atgcatcctg cgggaatatg tcttacgtga ttttcaacga tcaggtgatt aaaccgcgct
    60481 ttgaatgtaa gaccatctat gaaatgacca gcgaactggc aaaacgtctt ggcgttgagc
    60541 aacagtttac tgaaggccgt acacaggaag agtggatgcg gcatctgtat gcccagtcgc
    60601 gggaagcgat tcctgaacta ccaacgtttg aagagttccg caagcagggg atctttaaaa
    60661 agcgcgaccc acaagggcac cacgttgctt ataaagcttt ccgtgaagat cctcaggcaa
    60721 attcactgac cacgccatcg ggcaaaattg agatttattc gcaggcgctg gctgacattg
    60781 ccgctacctg ggaattgcca gaaggagatg tgatcgatcc actgccgatc tacacgccgg
    60841 gctttgaaag ttatcaggat ccgttgaaca aacagtatcc gctgcagctt acgggtttcc
    60901 actataaatc tcgcgttcac tcaacttacg gcgacgttga tgtgctgaaa gcagcttgcc
    60961 gtcaggaaat gtggatcaac ccgcttgatg cgcaaaaacg cggtatcaac aacggcgata
    61021 aagtcaggat ctttaacgat cttggtgagg ttcatattga ggcgaaagtg acgccacgaa
    61081 tgatgccggg tgtggtcgca ctgggtgaag gtgcctggta tgacccggat acaaaacgtg
    61141 tcgataaggg cggttgtatc aacgtactga ccactcaacg tccgtctcct ctcgctaagg
    61201 ggaatccgtc acatacaaac cttgttcagg ttgaaaaggt gtaaggagta accgatgaca
    61261 acccagtatg gattttttat tgattccagc cgttgcaccg gttgcaaaac ctgcgagctg
    61321 gcctgtaaag actacaaaga tttgacgcca gaagtcagct tccgccgcat ttatgaatat
    61381 gctggcggcg actggcagga agataacggt gtctggcacc agaacgtgtt tgcctactat
    61441 ctgt
//
LOCUS       DAJTSL010000006        56914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTSL010000006 DAJTSL010000000
VERSION     DAJTSL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.006"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1207)
                     /gene="wcaC"
                     /locus_tag="OO433_000334"
     CDS             complement(<1..1207)
                     /gene="wcaC"
                     /locus_tag="OO433_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6048944.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFY"
     gene            complement(1204..1676)
                     /gene="wcaB"
                     /locus_tag="OO433_000335"
                     /pseudo
     CDS             complement(1204..1676)
                     /gene="wcaB"
                     /locus_tag="OO433_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
     gene            complement(1679..2518)
                     /gene="wcaA"
                     /locus_tag="OO433_000336"
     CDS             complement(1679..2518)
                     /gene="wcaA"
                     /locus_tag="OO433_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR6048945.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGH"
     gene            complement(2696..4858)
                     /gene="wzc"
                     /locus_tag="OO433_000337"
                     /pseudo
     CDS             complement(2696..4858)
                     /gene="wzc"
                     /locus_tag="OO433_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
     gene            complement(4861..5304)
                     /gene="wzb"
                     /locus_tag="OO433_000338"
     CDS             complement(4861..5304)
                     /gene="wzb"
                     /locus_tag="OO433_000338"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6048946.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSWETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5310..6324)
                     /locus_tag="OO433_000339"
                     /pseudo
     CDS             complement(5310..6324)
                     /locus_tag="OO433_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
     gene            6633..6782
                     /locus_tag="OO433_000340"
     CDS             6633..6782
                     /locus_tag="OO433_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6048947.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            6983..8566
                     /locus_tag="OO433_000341"
     CDS             6983..8566
                     /locus_tag="OO433_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6048948.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(8840..10693)
                     /gene="asmA"
                     /locus_tag="OO433_000342"
     CDS             complement(8840..10693)
                     /gene="asmA"
                     /locus_tag="OO433_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6048949.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRNYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEVVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIHGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(10715..11296)
                     /gene="dcd"
                     /locus_tag="OO433_000343"
     CDS             complement(10715..11296)
                     /gene="dcd"
                     /locus_tag="OO433_000343"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6048950.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(11388..12029)
                     /gene="udk"
                     /locus_tag="OO433_000344"
     CDS             complement(11388..12029)
                     /gene="udk"
                     /locus_tag="OO433_000344"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6048951.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHNLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            12347..15644
                     /locus_tag="OO433_000345"
                     /pseudo
     CDS             12347..15644
                     /locus_tag="OO433_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(15683..16540)
                     /gene="alkA"
                     /locus_tag="OO433_000346"
     CDS             complement(15683..16540)
                     /gene="alkA"
                     /locus_tag="OO433_000346"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR6048952.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            16674..18026
                     /gene="yegD"
                     /locus_tag="OO433_000347"
     CDS             16674..18026
                     /gene="yegD"
                     /locus_tag="OO433_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6048953.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSNAAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(18038..19960)
                     /gene="yegI"
                     /locus_tag="OO433_000348"
     CDS             complement(18038..19960)
                     /gene="yegI"
                     /locus_tag="OO433_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCR6048954.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPASKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINVNGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FVDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALYDTARERQKQ
                     KFLEGFFASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKASC
                     DRRFVFRPNEAVTPADRQAVMAAKRHRLESALTVGATELQRFRLYAPARTMPLMEPLR
                     QAAEKLAQAQADLSRC"
     gene            complement(19957..20718)
                     /gene="pphC"
                     /locus_tag="OO433_000349"
     CDS             complement(19957..20718)
                     /gene="pphC"
                     /locus_tag="OO433_000349"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCR6048955.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASVTQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(20715..21374)
                     /locus_tag="OO433_000350"
     CDS             complement(20715..21374)
                     /locus_tag="OO433_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6048956.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(21595..21654)
                     /gene="ibsA"
                     /locus_tag="OO433_000351"
     CDS             complement(21595..21654)
                     /gene="ibsA"
                     /locus_tag="OO433_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCR6048957.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(21927..21983)
                     /locus_tag="OO433_000352"
     CDS             complement(21927..21983)
                     /locus_tag="OO433_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6048958.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(22255..22311)
                     /locus_tag="OO433_000353"
     CDS             complement(22255..22311)
                     /locus_tag="OO433_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6048959.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            22590..23837
                     /gene="mdtA"
                     /locus_tag="OO433_000354"
     CDS             22590..23837
                     /gene="mdtA"
                     /locus_tag="OO433_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR6048960.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGMIKVKARFNNQNDVLFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGNRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            23837..26959
                     /gene="mdtB"
                     /locus_tag="OO433_000355"
     CDS             23837..26959
                     /gene="mdtB"
                     /locus_tag="OO433_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR6048961.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLIFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYSAFGQRLISTIYTQANQYRVVLEHNTEITPGLATLDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            26960..30037
                     /gene="mdtC"
                     /locus_tag="OO433_000356"
     CDS             26960..30037
                     /gene="mdtC"
                     /locus_tag="OO433_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6048962.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGKLYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRAPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            30038..31454
                     /locus_tag="OO433_000357"
                     /pseudo
     CDS             30038..31454
                     /locus_tag="OO433_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            31451..32854
                     /gene="baeS"
                     /locus_tag="OO433_000358"
     CDS             31451..32854
                     /gene="baeS"
                     /locus_tag="OO433_000358"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6048963.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            32851..33573
                     /gene="baeR"
                     /locus_tag="OO433_000359"
     CDS             32851..33573
                     /gene="baeR"
                     /locus_tag="OO433_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6048964.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            33764..34096
                     /locus_tag="OO433_000360"
     CDS             33764..34096
                     /locus_tag="OO433_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6048965.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            34219..35619
                     /locus_tag="OO433_000361"
     CDS             34219..35619
                     /locus_tag="OO433_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCR6048966.1"
                     /translation="MLKGLRTLFSRRVLFNVEMNNSVDCELLLIQQQLQALIAEPGNY
                     LAHDCDLNDTLVLCQLTHHHNGYIREKAVQYLGHKEDVAAIEELLLAANDWVKEVRLA
                     ATIALRHLMKDKNAAAFAVNLPMIRNLLQCQRYDHRAFVDEILRYLLTENNRPQLTYW
                     LTCPDKKLSRAVLQTLIEYGYFNDESSLLSILDKPDEGLRMLATTHWLRQQLPLSEAV
                     LTRLLKDRWPRIRQATLFSLTDRAIEMPPALHSTLLLDNNMLIRLRAKNMLHEVMDVP
                     QFWRHVVTSAEYTPSQRRAALYGLDSIHDPNILKLAEWGLSQNVFPLRLAAMHILAKA
                     NPRCGVKETILTTLANPDAAGLRFMVNICVWCRVPLTFEEIRQLQENAPSVKHACAYC
                     RLYHNLNKWDGLILLLQSQHKLTEEFAGKQLAIWQRNFNLSGIQPNALQRQQLQALFT
                     RNPELHNRLWGYIPFK"
     gene            35940..37301
                     /gene="yegQ"
                     /locus_tag="OO433_000362"
     CDS             35940..37301
                     /gene="yegQ"
                     /locus_tag="OO433_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6048967.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREYFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGVAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(37631..37948)
                     /locus_tag="OO433_000363"
     CDS             complement(37631..37948)
                     /locus_tag="OO433_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6048968.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARSRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            38362..39260
                     /gene="yegS"
                     /locus_tag="OO433_000364"
                     /pseudo
     CDS             38362..39260
                     /gene="yegS"
                     /locus_tag="OO433_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
     gene            complement(39342..40115)
                     /locus_tag="OO433_000365"
     CDS             complement(39342..40115)
                     /locus_tag="OO433_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6048969.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(40221..41261)
                     /gene="gatD"
                     /locus_tag="OO433_000366"
     CDS             complement(40221..41261)
                     /gene="gatD"
                     /locus_tag="OO433_000366"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6048970.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITIGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGSMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(41309..42664)
                     /locus_tag="OO433_000367"
     CDS             complement(41309..42664)
                     /locus_tag="OO433_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6048971.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNIDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSVSLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFITWRRARGFIKQEKAVLA
                     E"
     gene            complement(42668..42952)
                     /gene="gatB"
                     /locus_tag="OO433_000368"
     CDS             complement(42668..42952)
                     /gene="gatB"
                     /locus_tag="OO433_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6048972.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(42983..43435)
                     /gene="gatA"
                     /locus_tag="OO433_000369"
     CDS             complement(42983..43435)
                     /gene="gatA"
                     /locus_tag="OO433_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6048973.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(43445..44707)
                     /gene="gatZ"
                     /locus_tag="OO433_000370"
     CDS             complement(43445..44707)
                     /gene="gatZ"
                     /locus_tag="OO433_000370"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR6048974.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIVRGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(44736..45590)
                     /gene="gatY"
                     /locus_tag="OO433_000371"
     CDS             complement(44736..45590)
                     /gene="gatY"
                     /locus_tag="OO433_000371"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6048975.1"
                     /translation="MHVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATEPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(45898..46950)
                     /gene="fbaB"
                     /locus_tag="OO433_000372"
     CDS             complement(45898..46950)
                     /gene="fbaB"
                     /locus_tag="OO433_000372"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6048976.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVVSTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            47207..48484
                     /locus_tag="OO433_000373"
     CDS             47207..48484
                     /locus_tag="OO433_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6048977.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPGTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFCESDNEITAIKVDDRDIALTQGEVK"
     gene            48481..49485
                     /locus_tag="OO433_000374"
     CDS             48481..49485
                     /locus_tag="OO433_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR6048978.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSITRHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            49482..50447
                     /locus_tag="OO433_000375"
     CDS             49482..50447
                     /locus_tag="OO433_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6048979.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(50421..51167)
                     /locus_tag="OO433_000376"
     CDS             complement(50421..51167)
                     /locus_tag="OO433_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6048980.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(51219..52037)
                     /locus_tag="OO433_000377"
     CDS             complement(51219..52037)
                     /locus_tag="OO433_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6048981.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQKVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(52102..52902)
                     /gene="thiD"
                     /locus_tag="OO433_000378"
     CDS             complement(52102..52902)
                     /gene="thiD"
                     /locus_tag="OO433_000378"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6048982.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIESDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQYV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(52899..53687)
                     /gene="thiM"
                     /locus_tag="OO433_000379"
     CDS             complement(52899..53687)
                     /gene="thiM"
                     /locus_tag="OO433_000379"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6048983.1"
                     /translation="MQVDLQSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLPHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(53722..53818)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(55399..55692)
                     /locus_tag="OO433_000380"
     CDS             complement(55399..55692)
                     /locus_tag="OO433_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6048984.1"
                     /translation="MYGQDTTAGGNLKKDSPLGKLWDFLHSPDLPTNPDFAKPADKTG
                     WVSSYSNPTYVPPTPTITAPQLKVDMAGNPLMTPPAWLNQPLNVNVNVKVQDG"
     gene            complement(55656..>56914)
                     /locus_tag="OO433_000381"
     CDS             complement(55656..>56914)
                     /locus_tag="OO433_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005135759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6048985.1"
                     /translation="MIEGAGGAITIAATVALSGKIMTTGQDFESALSGLTAITGSTDK
                     AAKEFDYLRDQSNRLGLDLLKTSRDYTQFAAAVGDKLPKDQMRSIFEGASEWGLVTGA
                     SADEASRALKSLNQMMSKGTVMSEELKGQLSEALPGAVGLFVKALNQMKGTTNLTEKD
                     LFDLMQQGKLFSKDILPEVAKQMKAAARNGGALDKAMKSNRASWQRLQTSMQNAMNIF
                     FASGFGNELTSAFDAISAAVNGSGGAFEMFGHIAGKIVEGATDAFTELHDTVILTFRI
                     IEYYASKMGITGEQLKAWGEMAAYAAGVAMFAGSIWKLGVAFKWLLTMLNPLTKLLGV
                     MKGIAAIGGIEAATTGGSGGEGKDKPNKPGRGPKVGKVGGAAALSGGPVGWSIAVGSV
                     GGDYAWDKVMTLTTPCMDRTPPPGAI"
BASE COUNT        13488 a        14964 c        14409 g        14053 t
ORIGIN      
        1 gatagaagtt gacatactcc tccagcatct gttgtccact gtaggcggcg cggctgcgct
       61 ggctgaaccc ggccaacgtg gtaccaaata tcgcctgcgc gatttccggt ttgcttaatt
      121 gcactagttg cagcacctct tcttcgctga cggttttacc gccggatttt tgcaacactt
      181 cccgcgccgc gtcgctatgg gtggcaatca ccggcacgcc aatcgatagc gcctcacaca
      241 aaatcagcgg gtagttatcg acgcgagaac tgaacaccag cgcatccatc tgattgagcg
      301 cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt aaccacgttg ccagcggtga
      361 acggcgagaa cttaccaaag gtatgcagtt cgattttgtc gcccagcgcc atcatctcac
      421 gcaccagttg ctggttagtc ttgccgtcgt aacgcaagtc atgcgccacc accgcgattt
      481 tcggcttgcc ctgggtttcg cgcaccggag gcaagtccgc cagaatcgct tcggttgcca
      541 tatcaatacc attattgata atccggcaac gccctggacc gtacaggcta ttgaaagcgt
      601 cagccacatg ctggctgggg gaaataaact gacagcccag cgccagcatc tcacggaata
      661 actggcgttt gcccgccacc agctggtgtg cgcgatcaat cttcaccggc ggatagttat
      721 tcaaggtcgg gcatttctgg cagcccgttt tccagccttc gcaaccgtcg gtaaaggcgc
      781 agcgtccggt aacactccag tggtcgtgca gcgtccagac cagagtgacg tccggtttat
      841 ggtttttcac tttttcgcaa aagcgcacca cgctcttaag atttagccag tagctgtgca
      901 gcacatgaaa atgcaggacc accggacccg gtgtgcgagt aatggtgcga tataactcat
      961 tgaaattgcc aaacagatcg cgattaaaca gacgaaacag ggcaatattc gccatcgcgg
     1021 tcatccgcgg cgtatgtttg atgacctgcg gatagttctg atggctgacg ctctctttgc
     1081 cgcctttgcc gtaaccgtag acaaaatgtg acgccagccc ctgttgcagc gcacgctggt
     1141 ggagatctaa cgccacacct gctgccccgc cttccgccag tcgcacatta aattgcagaa
     1201 tattcattta attaccttca ctcgcgcttt ttctcctacc accagcgcgt tgtccgggac
     1261 agagtcgagc accacgctgc ccgcacccac ggtgacgttg ttaccaagcg tgatatcacc
     1321 aagaataatg acgttggcac cgagttcgac gccgttgcca atgtgtggac atgccatgtt
     1381 atcggcacca cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat cccccgctac
     1441 cacgttttta ttgatcacta cggcgtaacc gtgatggata gtaaagcggc ggcctggatt
     1501 tcataaccga aaaagcattc ggtgataatg cgatacagca ccagcagcgg ggccgcccac
     1561 agattgttga ggacgttctt tttgcgccac accgaacaaa aatgagcaac acgataggca
     1621 agaaccatgc agcacgggcg taaactccag ctgttggcac gcagatcttc cagcatcttt
     1681 agtgcccccg aataccatca gccagacgtt tgccgttacg caccgacagc agcgtcagca
     1741 aagtgcgcca ggtcatacgt ttattgcgga tctggtagag ggtaaacagc tgatattttt
     1801 tgcttgcacg gtcaaattta tctttgtgct ttcggtaaaa gtggaagtac ccggagaatt
     1861 ttttcggtga cgaggtgatc tgcatttcac cgtgattgat atgcaggatc tgcgtcgctt
     1921 cttccacttt ccacggttcg ccgtactcca ccaccatccg caggaagatg tcgtaatcct
     1981 gtgctgcttt cagttcggta tcgaacaaac actctttgaa acgccatgcc caagtaaaga
     2041 cctgattgcc aatgatattg cgtttataga acaggcggcg tgaatacggc gatttgggat
     2101 atagcggcaa acttgctggt tgggaataga cttcgccctg gcaaacgtag tcgttagcgt
     2161 acaaaaaggc gtgagtgacc agttgctgtt tatgggcgag gaagacgctc agacggttgg
     2221 gtgtccattc gtcatcgtca tcaatcccgg tgatgtattc cccttgcgcc agcataatcg
     2281 cctggttacg taccgcgcac gccccgctgt taatatcgtt gtggatgtaa gtgatacgcg
     2341 gatcgttgag ggcggtgacg tactgttgca gttgttccca ggaggtggag caatcatcca
     2401 cgatgatcat ctcccagttg ctgtagtcct ggcgcaggac cgattttatc gcccgaatcg
     2461 ccagttgttg gcggttccag gtcggcatat agattgagat cagcggattg tctttcatag
     2521 gttgttctcc cggagtgtcg agatgttctg ccggtttata agcttgttta cgggttctgt
     2581 tgccggatgc ggcgtgagca ccttatccgg cctacggagc tggtttgctt gccggatgcg
     2641 gcgtgaaggt aggtcggata agatgcgtca gcatcgcatc cgacagcgga tgtcgttatt
     2701 tcgcatccga cttatattcg tattcgtaat agccataatc ctgatacgcg ctggcgcggc
     2761 ggaagatgga gttcagaatc acccctttca ccggaatacc gttttgctca aagcggctca
     2821 gactggtttc cacttctttc aaggtgttga ccgcataacg cgccaccatt aacgtggttc
     2881 cgacatgacg accaacaatt gccgcatcgg tcactgccag aatcggcggc gtatcaatca
     2941 acaccaggtc atagttttta ctcgcccagt tcaccagttc ggcaaagcgt tcgctcatca
     3001 acagttcaga aggatttggc ggcacctgac cgcgcgggat caggtcaaat ttggcaatag
     3061 aggtcggttt agcagcggta gtaatatcgc cctgaccaat cagaatttcc gacaggccat
     3121 taacgttatt agtgcacagc agctcgtggg tgtagccttt gcgcatatcg cagtcgatca
     3181 acaacacccg tttattggtc tggctgatca ccgccgccag gttggcgcag acaaaggttt
     3241 taccgattga cgggctaacc ccggtcatca tcaacacatt gttctgtgcc tgcatcatcg
     3301 cgaagtgcaa actggtacgc agactgcgga tggcttcaat cgccagatcg gttggattcc
     3361 ccatcgccag tagctggctc tgtttatagc gtttaatccc tttgatggtt ttgacgctat
     3421 cgcgcgcttt ctgccattcc gacagcggga tgctggcata gacgctgata ccgtgttctt
     3481 ccagcacctg cgggctttcg atgccgcgat taaacaacga gcgcagcagc acacccacga
     3541 tagaaagcat caggccaagg ataatcgcgc cgaggataat cagccctttc ttcggtttta
     3601 gcacgccagg ctgggtgatt gccgggtcaa caatgcgcac atcgccgacg gtgctggctt
     3661 cggtgatttt cagctcctgc tctttattca gcagttgcat atagacctgc tgaccagact
     3721 cgacatcgcg ggtcaggcgc acaatctcct gctgggtttt cggcatcgcc gttacgcgac
     3781 cgttaagttt ggctttctct tcttccagcg cctgacgttt ctccagcagc gtgcggtacg
     3841 ccgggtgaac tttggtgtac agcttggaga tttccgcctc tttaaaggtc agttcgttca
     3901 actgcgcgtc gatgttcacc atcgaatcga gcaccgcttt cgcttccagc ggcagatcaa
     3961 cagaatcttt atcctgacgg aaggcattca gtttgttttc ggcaacatcg aggcggctac
     4021 gtacttccgg taactgttgc gcgaggaaag cgaggctttt cgacgcttcc gccgatttgc
     4081 gctcaatatt ttgttcctga tagttacggg cgatgctgtt aagaatgtcg cggatctgtt
     4141 cgcgatcttc accggtataa gtcaggctca gtacgcctgc gtctttgccg ttctccgtta
     4201 ccatcaggct gttttgcagt tggttgatca tccccaacgt ggagtatttg gtgacggtaa
     4261 actcactgcc cgggcgggcg tgaatggctt caaccatcag cgtgacgcct tcttttttca
     4321 gcatctggcc cgcttgccca cgggcgctaa agccgccatc actgctcagg gtgtagtttt
     4381 tattgtccag cacattaagc gtaaacacct agtccgccat ctctttcggg cggttaaagg
     4441 tagtcacttt caccgtctcg ttctggcgtc ccatcagtcg atcccagccc gccccgaaaa
     4501 tcgggaacgt gtttttgctc actgcaatat cgaggtcgag atcgtccacc gttttaccaa
     4561 gcaccagacg cgaacgaatc aactggatct cggcgtccga tgcaggcggt ttgttggcta
     4621 atgccgaacc aatgtcctgc actaacgaat tgccgctgtt ttgctcgatt tgtaccagtg
     4681 cgtcggcact ataaatcggc gtggcgaaga aggtgtaaac cacggcacag aggacaaata
     4741 cagcggtgat gccaatcacc caccagcgcg cttcaatgac ggtgccgacc aggcgaccaa
     4801 tatcgatttc atcactgccc gttaccggag cggcatgttg ttttactttt tctgtcattc
     4861 ttatacctgc tctgcgttca atgcctgcgc ccactggcgg gcagaccgtt caagtaatgt
     4921 gtacaccgct gcaaacgttt cccagctttt gcgatacgga tcggggattt cacattcgtt
     4981 atcccagtga ccaaacagca tcactttgcc gcgcatctca ggtgccatct cgcataagcg
     5041 ttcgatatgg cgcttttcca tggtcaaaat caggtcatag ttgcgacaca ggcggcggct
     5101 gatttgtcgt gcacagtggc cttccagaga cagttgatgt tctgcggcga cgctgatagc
     5161 ggtaggatca gcgcccttac cgaccagcgc gccgagtcca gcggactcca ctttcagttc
     5221 cgggtgataa cgttgcagta agcgttccgc cgtcggggaa cggcaaatat tgccgacaca
     5281 gacaactaat atgttgttaa acatgacgat taccagttat gaatgtcgct ggctgtatcc
     5341 gtcatgtaac ggacaccgct aatagttggc aatcttgccg ttacgcccgc cctcgccttt
     5401 cagcggacga atgacaaaga tgccgctggc gttggaggtg gtcatgtcga taccttcagc
     5461 attacccagg gcttcagtca gggcttcagt cagggtcatg ccgctaaagt ccattttcag
     5521 ggtgctctgt ttcttcactt cacccatcac aaatactttc agatcatcat tacgcggcac
     5581 gtagagaata tcgccgggat aaagcagtcg gttctgattg aggtcgccgt tttgcatcag
     5641 cacctgcaaa gaaatgcgtt cttcacgacc attgtgtgtt agcaccacgt tgcgccagtc
     5701 agcggtgtcg gtcaggccac ctgcggcgtt gatggcgtcg agaatggtca gtggtacgtt
     5761 ggtgatcgcc tgttgaccgg atttattcac ctgacctgag atataggtct tttgtgagcg
     5821 gaaggcggcg atattaacgt ccacctgcgg gtcagcgatg tacgtcgcta agcgcccggt
     5881 aatatcactg cggatttcag cgagcgtttt cccgactacg tgaaccttgc cgatatacgg
     5941 gtaaaacata gtgccgtcag gctgtaccca gttgccggtg tcgctggagc tgcggtactg
     6001 accagctggc gtggtgagtt ccgggtgatc ccagacggtg acattaagaa cgtcccccgg
     6061 cccgacgcga tactggtaat tcgcgatctc gctttccagc gtcatattgg ggcgcgctac
     6121 attcgggcgt gggcgtaatt ggtcaatcag gcgcggggtc agcggataaa cattcaccat
     6181 tttgtcgaga tcgaaatcag cgtcctgctg tttgatgacg tctttgccca tcgtcgacat
     6241 attgctgccc ggaagtactg tgcaaccgct tatcaaggtt actgacacca ataatggcat
     6301 caatttcatt ttggatttca tcattgttta tttatcactt tggcagagta attatcctgt
     6361 gcactattaa tagcaatgtc gccatgcaca tttaccttgc agttaattga atacaaattt
     6421 aactggcatc agtcctaaaa aaattgattt catccgcagg ctattgacag aataattcag
     6481 actggtcttt caggcatcca gacacgctac cgcccctggc tttttagcca ccaatacact
     6541 gatttagctt aattttttaa accctctctg catacaatcg ttgatgagaa agggttattg
     6601 cggaaattaa cttccgaata taaggtgaca ttatggtaat tgaatattgg ctctccaata
     6661 atgcaggagg aagtgttaca gctaacggaa tagcaggcaa ggtaacaatt cggcaattgg
     6721 ctatttttaa gaattatatt aagtgtcatt caatatggtt tttaggagtt tctttaggtt
     6781 gacaatattt aatattgcgc ctccgcatgc gatatttctt aaataatgtt ttattattac
     6841 cacttttaat tcagggataa tgtgaggtta ttaccctcaa ataatatgag ataaatagtg
     6901 ctgcaacatt gcatttttgc cccgatttat ccatgatcga attgtgacat ttgtcataca
     6961 acgaataggt tttgtactta ctatggaatg gattgccgat ccgtctatct gggccgggtt
     7021 aatcacgctg attgtgatcg aactggtcct cggcattgat aacctggtct ttattgccat
     7081 cctcgccgaa aaactaccgc cgaagcagcg cgaccgcgca cgggttaccg ggctgttgct
     7141 ggcgatgtta atgcgcctgt tactgctggc gtcaatctcc tggctggtca ccctgacgca
     7201 accgctgttc agcttccgct cgtttacctt tagcgcccgc gacttaatca tgctgtttgg
     7261 tggtttcttc ctgttgttca aagccacgat ggagctgaac gaacggctgg aagggaaaga
     7321 cagcaataat cccacacaac gcaaaggtgc gaagttctgg ggggtggtga cacaaattgt
     7381 ggtactggac gccatcttct cactcgactc agtgattacc gccgtcggga tggtcgacca
     7441 tttactggtc atgatggccg ccgtggttat cgctatcagc ctgatgttga tggccagcaa
     7501 gccgttaacg caatttgtta acagtcaccc gaccatcgtt attctctgct taagcttcct
     7561 gttgatgatt ggctttagcc tggtggcaga aggtttcggc ttcgtcattc cgaaaggcta
     7621 cctgtacgct gccattggtt tctcggtaat gatcgaggcg ctcaatcagt tggctatctt
     7681 taaccggcga cgttttcttt ccgctaacca gacgctacgc cagcggacta ccgaagcggt
     7741 aatgcgcctg cttagcgggc aaaaagaaga tgcggaactg gacgccgaaa ccgcgtccat
     7801 gctggtggat catggtaacc agcagatctt taatccgcag gaacggcgga tgattgagcg
     7861 ggtacttaat cttaaccagc gtactgtcag cagcattatg acgtcgcgcc acgatattga
     7921 gcatatcgat ctcaacgcgc cggaagacga gatccgccag ttgctggagc gaaatcagca
     7981 tacgcggctg gtggttaccg acggtgatga cgcagaagat ttgctcggtg ttgttcacgt
     8041 tatcgacctg ctacaacagt cactgcgtgg cgaaccgctc aacctgcggg tgttgattcg
     8101 ccagccgcta gtgttcccgg aaaccttgcc gttgttacct gccctggagc agttccgtaa
     8161 tgcccgcaca cactttgctt ttgtggtgga tgagtttggc tcggtggaag ggattgtgac
     8221 attaagtgac gtcactgaaa ccattgccgg taacttaccg aacgaagtgg aagagatcga
     8281 cgcccgccat gatattcaga agaatgcgga cggttcctgg acggcgaatg gtcatatgcc
     8341 gctggaagat ctggtgcaat atgtgccgct gccgctggat gaaaaacgtg aatatcacac
     8401 cattgccggg ctgttgatgg aatatttaca gcgtattcca aaacctggcg aagaagttca
     8461 ggtgggggat tatttgctta aaacgttgca ggtggaaagc catcgcgtgc agaaggtgca
     8521 gattattccg ctgcataagg atggcgagtt ggagtacgag gtgtgatggt atcggtcatt
     8581 ttacccaata gccgaaagga ataagatgag ggaaccgcgc cagcggcgat ttaccgccgg
     8641 gagcccgggt tgccagggtg gtggcgattg agcccccctg gcacgttcac cggcttgagc
     8701 gttccatagt agcgaggaac atcaagtgaa cggaacagcc ttcgatctta catattcaga
     8761 atataatgat gccgccacga acgcctgatg cgacgcttta cgcgtcttat caggcctaca
     8821 ggacaggtca ccaggtaacc tacatcttct ccagcaactt cttcacatct ttgccattgc
     8881 gggaatcttt attccgctcg gcccagtcat tcagacgacg tttcgcttcg tcctgtagat
     8941 gtttgcgcag taactgatcc acttgcagac tgtaattgag ttgctgccag ttgccataaa
     9001 cccgcagcgg tactggcgtt tctttaagga aatcaatcag tttgctttcc ccgttccagc
     9061 cacccacgac ccgaatatca aactgggtgt cgcaggtttg ctccgccaga ttcaacgtac
     9121 cagccccgga aagcgccagc acaggcgatt gaccttgcat gtcgtttaac gtcacgacgc
     9181 catccttcaa cgtcaaatcg gtggtaaagc ggtcaagacg cgttacgtta tcgaagtttt
     9241 cagcggcctt cacatcacca ccattacgtt ctaccgcttg ctgaatcatc tgctggaagt
     9301 tcatcccttc catgcgcgtg tcggtcattt cgacatgtgc ctgtccttgc cagttgtggc
     9361 ggaatgcgtc ggcatctatg tcagcaccgg agaaatcacc agccagtgac atctttccgg
     9421 tcaacgaaat cggatagtta aacgccttca gaatggtgcc aatctcaacg ttttccagcc
     9481 gtggctggaa gtttatccgc ggatttattg atgtcgcgtc cagcgtgccc ggcagtgaaa
     9541 cctgtccacc gttaagcttg ccctgcagtt gagtaatttc cagcaaaccc gacttgttgg
     9601 tcatttgcgt ggcaacatct gtaaaattca ttccgcgcca gcgcacgtta ctggcctgca
     9661 acaaaatatc agccgtaaag ccttgcagtc cctgatatgc cggttcatca atacgcgaag
     9721 aaatgaccgg gcgcggcaac gttgattggc tctgcccctg ctgcgcggca ccgttttcac
     9781 cattcgctgt ttcattaagc gggatgaggt tgtcaagatt cagttgcggg aattgcagtc
     9841 tcagctgcca ttccggtttc tctgtcagcg tgacctgtgc ttgcccgctc agcgtactgt
     9901 cattggcggt caaactaatt tggttaaatg aaaggcgttt atgtgactcc tgccactggg
     9961 cctggaaact cccctgccca tgaatacctt gttttggtaa atctgcaccc tgcaactgcc
    10021 agttaatttg ttcaatagcc gccgttaaat catgcggata atcagaagca tctaccgtac
    10081 cgttaaggga tatcgtgaga tcgcgctgat cgcgattaac ccgcccggag aactcaaatg
    10141 aaccacgatg ttggggatct tgttccattt gcaggcgaat attgcggatt gtcacctgct
    10201 cgtcatcttc atgctggaac accagcacgc tgtccgccac cttaagactg gatatatcaa
    10261 acgaccatcc gcgatcgtct gacagatccg gcaaggtatt gtcgcgcggt gcaaccggag
    10321 cgtcttcact gcgcaccact tccgtctgcg gcgtcagttg gatcactgcc ccttttagca
    10381 tcacctgctt aacgctcagt tgatgactca gtagtggtaa aagcgccacg tccagacgca
    10441 tgttgtcggc gcgaaccagt ggctggctcg cgccctgggc ggtgagagac attcgcccag
    10501 agaggatact aagctgcggc cagacgtgcc aacgcagtgg cccgtcgagc tgcaattgat
    10561 aaccgctacg tgcagcaact tgcttgacca tatagttgcg gaaatcattc ggattcacca
    10621 gcaacactaa cgcagataac ccggccacca gcacgaccag gagtatcatc agcgtcgtca
    10681 gaaatcgtct catggtatcc tcaatgggcc tgaattagtc tttatcgatt cggctggcta
    10741 ccgcgccctg ctggttgcga tatttcgcat cttcacggcg gttgtaaggt cgcgccgccg
    10801 ggccggaaag cggctcaaag ctcagcgcac caattaacat gcccggacgc agcgccagcg
    10861 gcagcttacc ggagttgtag aactccagca caatgcaacc agaccagccc ggatcgatac
    10921 ggtgcgcggt gacgtgcacc atcagcccca gacgcgccaa agaggaacgc ccgtccagcc
    10981 agcccaccag atcggctggc agcgtcaccg actccagcgt caccgccagc gccagttctc
    11041 ctgggtgaag atagaacgcc tcgccctcgt cgagaacgat ctcatcgctc atcacgcggt
    11101 caagcgcggc gctcacttca tctttgggac cgctcagatc gataaacgct gccgtgtgac
    11161 cacggaaggt acgaaattta ttgcccaggc gtacatccac cgtcgcgccg ttaatacgct
    11221 ccactggcgg acgtgggttg atcgacaaac ggccttcatc aagccaggct tcaatatctc
    11281 ggtcacacag acgcatggca ttttctcctt tcgcgcatca ctcccttaac gccaattacg
    11341 tcaagggcat accaggttat cactgaacgg tacacaattt atcaagctta ttcaaagaac
    11401 tgacttattt tcgctttcaa tatatcgatc gcgatgcggt ttttcccgcc gcgcggcacg
    11461 ataatgtccg catattgttt agaaggctca atgaattgca ggaacatcgg gcgcacggtt
    11521 ttttgatatt gcgccatcac ggaatccatt gaacgcccac gctcgttaac gtcacgcttg
    11581 atgcggcgca tcaggcagat atccagcggg gtatcaacga aaatggagaa gttaagttcg
    11641 tcacgcaaac gcgcatccgt cagcaacaaa atgccttcga gaatgatgac cttcttcggc
    11701 tcaaccgtca ccgtttcttt catacgcgta tgttcaacat agctgtaaac cggcaggtca
    11761 attgccgagc cgcgtttcaa cgcttgtaaa tgctcaagca gcaggttgtg atccatcgcg
    11821 ctgggatggt cgtagttggt cttaacgcgt tcttccatcg acagatggct ttgatctttg
    11881 taatagcagt cttcgggaat tacgccgatg tgttcatcac cgacttgctc acgtaattca
    11941 cgataaaggg tactggcaat aagactcttg ccggaagccg atgcgccagc gataccgata
    12001 atgacgcact gatgagactg atcagtcata tatttagcga cctgattaac ctggatgtta
    12061 ggaagggggc gacgaagcgc caaacgcggc aattataggg atttcatccg cctgatacca
    12121 gtcgaatagc gttgccgcgc gctcagagtt aattgttgac aaagaattcc cggtggcaaa
    12181 ttacgttgat cagttttatg taaggtaaaa aatgttatac gcagttgcgc aaattatccg
    12241 cctttacgtc actttatgag caattcacat ataaaatgta aaacttttgt actagcataa
    12301 acacagaaac gaatactggc gacctggtct tgcggataaa gcggtaatga gcaaacaatc
    12361 acagcatgta ttaattgccc tgccccaccc actgcttcac ctggtcagtt taggtttagt
    12421 ctcgtttatc tttacccttt tctcgcttga gctttcgcag tttggcaccc aactcgcccc
    12481 actgtggttc ccgacgtcca tcatgatggt ggcgttttat cgccatgccg ggcgcatgtg
    12541 gccgggaatt gcgctgagct gctcgctggg aaatatcgcc gcatccatcc tgcttttttc
    12601 caccagctcg ctgaacatga cctggacgac catcaatatt gttgaagccg tggtcggggc
    12661 agtgctgcta cgtaaattgc tgccgtggta taaccccttg caaaatctgg ctgactggct
    12721 gcgtctggca ttcggcagcg ccattgttcc gcctctgtta gggggtgttc tggttgtcct
    12781 gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt cagaatccat
    12841 cggcgctctg gtactggtgc cgctgggatt gttatttaag ccacactatc tgctgcgcca
    12901 tcgcaaccca cggttacttt ttgagtcact gctcacatta gccatcacgc tgacgttaag
    12961 ctggctttct atgttgtacc tgccgtggcc ttttactttc attattgtgc tgttgatgtg
    13021 gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca cggtgatgat
    13081 ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta cgtacctgat
    13141 gagccatatg ccgtggctac tgtttttgct gatcctgctg cccgccaaca tcatgaccat
    13201 ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa cccgctttcg
    13261 gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg gacaatggct
    13321 gcaaaccaac aaagcgctct gccagtttct cgggtacagt caggaagagc tgcgcggact
    13381 cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac aggttgaaaa
    13441 gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca accgtaatgg
    13501 cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg gcacgccgct
    13561 ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac aggtgaatca
    13621 gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca tctgggagtg
    13681 ggagctgaag ccgaatattt ttcgagctgt atgaaattcc tccgcatatc aaaccgaact
    13741 ggcaggtgtg gtacgagtgc gtgctgccgg aagatcgcca gcatgccgaa aaagtgattc
    13801 gtgattcgtt gcaatcacgc tcgcccttta agctggaatt tcgcattacc gtaaaagacg
    13861 gtattcgcca tatccgcgcc ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac
    13921 gcctgctcgg cattaatatg gatatgaccg aggttaaaca gcttaacgag gcattgtttc
    13981 aggaaaaaga gcgcctgcac attacgctgg attccatcgg tgaagccgtg gtctgtattg
    14041 atatggcgat gaaaattacc tttatgaatc cagtggcgga gaagatgagc ggctggacgc
    14101 aggaagaagc attaggtgtt ccgctcctga cggtgttgca tattactttt ggcgacaacg
    14161 gaccattaat ggagaacatt tacagtgccg acacctcacg ttccgcgatt gaacaagatg
    14221 tggtgttgca ctgccggaac ggcggcagtt acgacgtgca ttacagtatt acgccgttaa
    14281 gtactctgga cggcagcaat ataggctcgg tactggtgat tcaggacgtc accgaatcac
    14341 gcaaaatgct gcgccagctg agctacagcg cctcccatga tgcactgacg catctcgcca
    14401 atcgcgccag ttttgagaag caactgcgta tcctgctgca aacggtaaac agtacacatc
    14461 agcgacatgc cctggtgttt atcgatcttg atcgctttaa agcggtgaat gacagcgccg
    14521 ggcatgcggc gggtgacgct ttactgcgcg aactggcgtc gttgatgctg agtatgctgc
    14581 gctccagcga cgtgctggcg cgactcggtg gtgatgaatt tggtctactg ttgccagact
    14641 gtaatatcga aagtgcgcgt tttatcgcta cacgcattat cagtgccgtg aatgattatc
    14701 actttatatg ggaaggccgt gtgcatcggg taggtgccag tgccgggatt accttgattg
    14761 atgacaacaa tcatcaggcg gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct
    14821 ccaaaaatgg tggacggggc cgggtgacgg tttacgaacc acagcaagct gccgcacata
    14881 gcgaacgggc ggcgatgtcg cttgatgaac agtggcggat gattaaagag aatcagttga
    14941 tgatgatcgc ccacggtgtc gcttcgccgc ggatcccgga agcgcgtaat ttgtggctga
    15001 tttcacttaa gctctggagt tgcgaaggcg agattattga tgaacaaaaa tttcgtcgta
    15061 gcttcagcga tccggcactt agccatgctc ttgaccgacg ggtattccac gaatttttcc
    15121 agcaggccgc aaaagcggtt gccagtaaag gcataagcat cgccctcccc ctttccgttg
    15181 ccggtttgag tagcgccacg ctggtgaatg atctgcttga gcagctggga aatagccctc
    15241 taccaccacg gttattacat ctgattattc cggctgaagc gattttagat cacgcagaaa
    15301 gcgtgcaaaa actgcggctg gtgggatgtc ggatagtgct cagccaggtg ggccgcgatc
    15361 tgcaaatctt caactcgctg aaagcgaata tggcagatta cctgctactt gatggtgagt
    15421 tatgcgccaa cgtgcagggt aatttgatgg atgagatgct gattacgatt attcaggggc
    15481 acgctcagcg actcgggatg aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg
    15541 atacgctttc tggcatcggc gtcgatctga tttatggtga tgtgattgcc gatgcccaac
    15601 cgctggattt gctggtgaat agcagttatt tcgcgattaa ctgagagagc gtttgcccat
    15661 tgcctgatgc gacgtatcag gcctacaatt cgtccgttcc gtctggttgc cagccttccg
    15721 tataccagat atgcaacagc gcataagaac gccagggctt ccagcgctcg gcataacggc
    15781 ggatttgtgc cggtgtcatc cccggaaatc gctgtttaat cagataatca tccggcagaa
    15841 aaacatcttt cgcctgccag ccacgcaaag cgaaataatt cgccgtccag cgcccgatac
    15901 ccggaaaagt ttgcagcgtt ttcatcgcct gctccacatc gcccggaatt gtcattggta
    15961 agctgccctc cagcgccgca tttgccagat gaatcagtgc ctctgcccgt ttcaacggca
    16021 tgcctaacgc ttttaatgcc tgcgggtcgg ctgctgccag ccgctgaggc gtggggaagc
    16081 agatatactc cggaaaatca tccagccgtt cgccataaag ctgtacaact ttggcagtca
    16141 attttgccgc catcgccacg ctcaccagtt ggcctaaaat cgcccgcaca ccctgctcaa
    16201 aagcatcaat acagccgggt aaacgcaatc cgggccgcgc cgcgcctaac ttgcccaacg
    16261 caccgttaac aatctgcggg ttacattgca gatcaaacag gcggctcatt ttcgccagac
    16321 actcagcggc aacgggttct aaacctgcac ttaaatttat gtgcagagta tggcgggcta
    16381 tatccggaat agcagtcacc acgccgcgat attcgcccac cgccagacta cgggcataat
    16441 aatcgtcagc gaccgtttcc acaccactca ctgcacgggc ggcgagaaat cccaacatcc
    16501 acgaccagtc atacggcggc tgccagttca gggtatacat cgcatctcct tattcatccg
    16561 ctttcagcat aaacgttatt cagacgctac gctttgcttt catattccgg ttgtcgcgac
    16621 ggcaacattt cgctaaagtc acgccccttc ttcaccggca tggggattat ttcgtgttta
    16681 ttggttttga ttacggtaca gcaaactgtt cagtggcggt catgcgtgac ggtaaaccgc
    16741 acttgctaaa aatggaaaac gacagcacgc tgctgccttc aatgctttgc gcgccaacgc
    16801 gtgaagcggt aagcgaatgg ctgtaccgcc atcatgacgt tccggcagac gacgatgaaa
    16861 cgcaggcgtt gttacgacgg gcgattcgtt ataaccgcga agaagatatc gatgttacag
    16921 cgaaaagcgt gcagttcggt ctttcctcac tggcacagta cattgatgat ccagaagaag
    16981 tgtggtttgt gaaatcacca aaatcgttcc tcggtgccag cggcttaaaa ccgcagcagg
    17041 tagcgctgtt tgaggatctg gtctgcgcaa tgatgttgca cattcgccag caggcgcagg
    17101 cgcagctgcc agaagcgatt actcaggcgg tgattggtcg cccgatcaac ttccaggggc
    17161 tgggcggcga tgaagcaaac gcccaggcgc aagggattct ggaacgcgcg gcgaagcgtg
    17221 ccggattcag ggacgtggta ttccagtatg agccggtcgc ggctgggctg gattacgaag
    17281 ccaccttgca ggaagaaaaa cgggtgctgg tggtggatat cggtggtggt acgactgact
    17341 gttcattgct gctgatggga ccgcagtggc gttcgcgtct cgatcgtgaa gccagcctgc
    17401 tgggtcacag tggttgtcgt attggcggta acgatctgga tatcgccctg gcgtttaaaa
    17461 acctgatgcc actgctgggt atgggtggcg aaaccgaaaa aggcatcgcc ctgccgatcc
    17521 tgccgtggtg gaatgcggtt gccatcaacg acgtacctgc gcagagtgat ttctacagta
    17581 gtgccaacgg tcgtctgctt aacgatctgg tacgcgatgc ccgcgaaccg gaaaaagtgg
    17641 ccctgttaca gaaagtctgg cgtcagcgtt taagctatcg cctggtacgc agcgcagaag
    17701 agagcaaaat cgccctttca aacgctgcgg aaacccgcgc ctcactgccg tttatcagcg
    17761 atgaactggc tacgctgatt agccagcaag ggctggaaag cgccctcagc cagccactgg
    17821 cgcggattct ggaacaggtg caactggcgc tggataacgc ccaggaaaaa ccggacgtta
    17881 tatacctgac cggcggtagc gcccgttcgc cgctgattaa aaaggctctg gcagaacagt
    17941 tgccgggcat tccgattgca ggcggcgatg actttggctc cgtcaccgcc gggctggcac
    18001 gctgggcgga agtggtattt cgttaaaaga aaatggctca gcagcggctt aaatctgcct
    18061 gcgcctgagc cagtttttct gccgcctgac gtaacggttc catcaacggc atggtccgtg
    18121 ctggagcata aaggcggaat cgctgcaact ctgtcgcgcc gacagtcaac gccgattcca
    18181 gccgatgtcg tttggcggcc attaccgcct gtctgtctgc tggcgttacc gcttcgttcg
    18241 gcctgaacac aaaacggcgg tcacagctcg ctttccagtc gataaccgcc tgggtcagat
    18301 ggtcgccaaa ccctttaact tgcttaacgc cacgacgggt aacatccgct gctgtttcaa
    18361 taccaaaaga ccgtaacgcc gctttacgcg cagggccaac gccgggaata gaggcaaaaa
    18421 atccctccag aaacttctgc ttctgccgtt cccttgcggt gtcataaagt gctgccagag
    18481 cgcgtttctc ctcttccggt aacccgagaa tttcgtcctt cattttttcg agcatcgccc
    18541 gtttggcgat aaaaccttcc agcccgccca actgttggat ctggctgact aaatgctcat
    18601 agtccatttt cgcgcgatta aatgcctcac ggcgttgctg gatttctgct ttgtacactt
    18661 tgcttgtcag actgccaata atccagatag ccgacagcac aggaaccaaa ataatataac
    18721 gcggttctgc ctgaaggccg caaagcaaca gcgataacgc tgacagtgcg atctcaatca
    18781 gaatgatgta ttcgcgccgt aacaggccta aaggaagcgg cctgccagtc ggttggaaat
    18841 gatcgggtaa tggcaattgc aatgctggcg gtgctactga cgccatcacc atcgcccaga
    18901 ctttcgccag cacaaaatca ccgctggtgg taatgacctc ttcaccgaga tcaacaaaat
    18961 agataacgcc ttgattatcc agcgtacacc acgggcagtc ggtcaaatga gcgggataaa
    19021 catgcattgc cgaaacggga catttcttta actgttggcg cagagaatca agtgccgcta
    19081 cccacgcttt agccgtcgga cgcccggttg ccacgccact ttccgtgaat gcctgctgaa
    19141 acatggcttc aacatcactc ggtaacatcg atagcggaat cgatcgtggc ggcggtttta
    19201 aaccacgtcg ctgattatct gacgcgtagg cataacggaa atgggtaata tccgtctcca
    19261 gcgcattacc cgcatcagag ataagcggca caccagaata aggatgccgg ccaccaaaca
    19321 agacgtgaaa aatcagcaac gcaaggccaa aattatcgtg attttctgtg cgttcaaagc
    19381 cgacaaatga cgacaaggtt tgtagctctg gcggcgtaaa atgcgacacg ccgacttcgc
    19441 ataaatgcag tgtgccattg acgttaatct gaaaggagtc gctatcaatc agtaccactt
    19501 tgctgtcgcg acctaccata aaactgttct ggtttacgtc acccacgacg tgcccgtgct
    19561 cgtgaaccgt agcaaaagat gaagcaatat tgcgcgcaac atagagtaga aaatcccacg
    19621 cacaatgagg gtaactctgg cgacgatgtg ccgggctata gatcatatga atcggttctt
    19681 taccagaaac ttttggcatc ataaaaccga taacttttcc gcctcgtcca ccgtgaagcg
    19741 ttgcctgcgg ccaggcgaca taattcaaca actgcgcgtc agctgtcgca gccataaagg
    19801 caagtttgtc ctgttttgag gcgggtggcg gcgtgtgata aatcttggcg acgctatcga
    19861 caaactcctc gatatcataa accgcgcctt caccgccttt gcccagttca cggcctggcg
    19921 taacgcactc accagtagca gtatataaag tgggtttcat ggtaaccaca gcgccagtgc
    19981 caacgtttta tcatcatccg tacgctcgtt taccgccggg ctggacaaaa actgctttaa
    20041 caattcaggt aataaatcga gttgttcctg cgttactgac gccagtccat tgaaaaacgg
    20101 ggtaaagaaa ggcacatgag gagaattatc cagcatattt aacgccagcc gctgaatacc
    20161 atccgtaaat gctgcaactt tatgcgcacg cccagtgctg gtgaaagttt ccagtctgga
    20221 aacggcatct tcgtcggtaa tgaagtgcgt catgttggca tattcaccca ccatcggcgt
    20281 gagaggcaat tgcagaccgt gacccaaatc taccaccacg ccgccatcgc caatctgcat
    20341 aatcaaggtg ccgtcaggcg atgaaatcaa tcccagaaac gtgcaggcaa aatcgcgaac
    20401 tgctaatgct ttggcttctg cctcggcaaa aagtcgttga cgaatagtca gtaccatatt
    20461 tgtggcgagg acatcattaa gccccaattc accgtcctgt actttttgca ccataaacgc
    20521 catcgcttca ttgacggcga gcgtcgcgcc ttctcctccc tgcgaaacgc tacctgcgcc
    20581 atcagcaagg aacatcacca gtaatggttg ctggtcgttt aaccagacaa cctgcatctg
    20641 gcaggcatcc tgacaaggca aatcagcact aatgtgcgat gttccaacag cggatgcata
    20701 aaccagacgc cagctcacac tgacgtccag ccttttggcg cttccagcac aacttccgtt
    20761 cccggcgtgg agcgggaaac cgaacgcagc gaactggata accagctaaa cagttcacgg
    20821 aattgtaatc cctgcagtgg caaaggctga cgaacgctga tttgtgccag agtcttcata
    20881 tccgccccct gaacaccgat ggaaaagaag gcaaacttct tatcttcttc cccctgaaac
    20941 actttgttgg cggcagcctg ccactcatcg gttggagcgc catcggtgat caggaaaatc
    21001 cacgggcgat aatacgaaat accatttgcg cggtactcac gtttcctctc ttcaaccata
    21061 tctagggctt tggtaatggc ggcccccata ggcgtatccc cctgggcaaa caggatgggc
    21121 gggaaaaagt tagcggcgct ggtaaagggt tgttccacat gcaccgggcc gaacgtcaca
    21181 atgccgagtt ccactctttt taaagccagc ggatcagcaa gcagttcatc acgaaaggta
    21241 accaatccgg cgttaagttc attaataggt ctgccgctca tcgagccaga aacatccagc
    21301 agcaaaatgc aggggcaacg tggttccggg ttactggcaa aatcgctggt ggcaaacgtg
    21361 atttgttcgc tcataagaaa tcctttctta gagaattatc gaggtccggt atcataccga
    21421 ttgcaataat gaaataagag caaacgaaga aactttcaga attaggcccg tcggcgcaga
    21481 aaattttgct attcgcctcg caagaacgga gagccgtcga ttgacactaa gggcggagtg
    21541 acataatttc aggagtgagg gttagggaga ggtttccccc tccccctggt gttcttagta
    21601 agcctggaag ctaatcacta agagtatcac cagtatgatg acgtgcttca tcataaccct
    21661 ttccttatta aaagccctct tctccgggag aggctttccc gtttcagcgt cccgctgaaa
    21721 tcatcggctt acctcctttc gccctgaatg cagtctatcg ctaacgcgtg aataaaatag
    21781 tttcctctgt gattattgcg taacgactcg caaagaatat ttgctggcag gatcgcagac
    21841 tacaaagcct acggattgac aatcttatcg tgaaggcata ctttcaggag tgagggtaga
    21901 gcggggtttc ccccgccctg gtagtcttag taagcgggga agcttatgac taagagcacc
    21961 acgatgatga gtagcttcat catgaccctt tccttattta aggccccttc ctcgggaggg
    22021 gctttcccgt ttcagcgtcc cgctgaaatc gtcggcttac ctcctttcgc cctgtatgca
    22081 gtctatcgct gacttgcgga taaaatagtt tccagtgtga ttattgcgta acgtctcgca
    22141 aagaatattt gctggcagga tcgcagactg caaagcctgc agattgacaa tcttatcgtg
    22201 aaggcatact ttcaggagtg agggtagagc ggggtttccc ccgccctggt agtcttagta
    22261 agctggccag ctaatgacta agagcaccac gatgatgagt agcttcatca tgaccctttc
    22321 cttatttatg gccccttcct cgggaggggc tttcccgttt cagcgtcccg ctgaaatcgg
    22381 cggcttacct cctttcgcca tgcaagcagt ctatccctaa cgcgtagata aaatagtttc
    22441 ctgtgttatt actggatgcg tgctcgcaaa tgtgcccgtc attcagacaa ttcccgacag
    22501 tgtttcataa ttcctccatt tttctccctt attggctggc tacactagta tcattccgcg
    22561 aaacgtttca ggaagagaaa ctcttaacga tgaaaggcag ttataaatcc cgttgggtaa
    22621 tcgtaatcgt ggtggttatc gccgccatcg ccgcattctg gttctggcaa ggccgcaatg
    22681 actcccggag tgcagcccca ggggcgacga aacaagcgca gcaatcgcca gcgggtggtc
    22741 gccgtggtat gcgttccggc ccattagccc cggttcaggc ggcgaccgcc gtagaacagg
    22801 cagttccgcg ttacctcacc gggcttggca ccattatcgc cgctaatact gttacggtgc
    22861 gcagccgcgt ggacggtcaa ctgatggcgt tacatttcca ggaaggccag caggtcaaag
    22921 caggcgattt actggcagaa attgacccca gccagttcaa agttgcatta gcacaaaccc
    22981 agggccaact ggcaaaagat aaagccacgc ttgctaacgc ccgccgtgat ctggcgcgtt
    23041 atcagcaact ggcaaaaacc aatctcgtct cccgtcagga gctggatgcc caacaggcgc
    23101 tggtcagtga aaccgaaggc accattaagg ctgatgaagc aagcgtcgcc agcgcgcagt
    23161 tgcaactcga ctggagccgc atcaccgcac cagtcgatgg tcgcgttggt ctcaagcagg
    23221 ttgatgttgg taaccaaatc tccagtggtg ataccaccgg aattgtggtg atcacccaga
    23281 cgcatcctat cgatttagtc tttaccctgc cggaaagcga tatcgctact gtagtgcagg
    23341 cgcaaaaagc cggaaaaccg ctggtggtag aagcctggga tcgcaccaat tcgaagaaat
    23401 taagtgaagg cacgctgtta agcctcgata accaaatcga tgccacaacc ggtatgatta
    23461 aagtgaaagc acgctttaat aatcagaatg atgtgctgtt tcccaatcag tttgttaacg
    23521 cgcgcatgtt agtcgacacc gaacaaaacg ccgtagtgat cccaacagcc gccctgcaaa
    23581 tgggcaatga aggccatttt gtctgggtgc tgaatagcga aaacaaggtc agcaaacatc
    23641 tggtgacgcc gggcattcag gacagtcaga aagtggtgat ccgtgcaggt atttctgcgg
    23701 gcaatcgcgt ggtgacagac ggcattgatc gcctgaccga aggggcgaaa gtggaagtgg
    23761 tggaagccca gagcgccacc actccggaag agaaagccac cagccgcgaa tacgcgaaaa
    23821 aaggagcacg ctcctgatgc aggtgttacc cccgagcagc acaggcggcc cgtcgcgcct
    23881 gtttattatg cgtcctgtcg ccaccacgct gctgatggtg gcgatcttac tcgccgggat
    23941 tatcggttat cgcgccctgc ccgtttcggc gctgccggaa gtggactatc cgaccattca
    24001 ggtggtcacg ctctacccag gtgccagccc ggatgtcatg acctctgccg ttaccgcgcc
    24061 gctcgaacgc cagttcgggc agatgtctgg cctgaaacag atgtcgtcgc aaagttccgg
    24121 cggtgcgtca gttatcactt tgcagttcca gctaacatta ccgctcgatg tcgccgagca
    24181 ggaagtgcag gccgcgatta acgctgcgac caacttgttg ccgagcgatc tgcctaaccc
    24241 gccggtttac agcaaagtga acccggcaga tccgccgatc atgacgctcg ccgtcacctc
    24301 aaccgccatg ccgatgacgc aagtggaaga tatggtggaa acccgcgtcg cgcagaaaat
    24361 ctcgcagatt tccggcgtcg gcctggtgac gctttccggc ggtcagcgtc cggctgttcg
    24421 cgtcaaactt aacgctcagg cgattgccgc cctcggcctg accagcgaaa ccgtgcgcac
    24481 cgccattacc ggcgctaacg ttaactcggc aaaaggtagc ctcgacggcc cttcccgtgc
    24541 ggtcacgctt tccgcgaacg accagatgca atccgccgaa gagtatcgcc agctaatcat
    24601 cgcctaccag aacggcgcgc caattcgtct gggcgatgtc gcaactgtag agcaaggtgc
    24661 agaaaacagc tggctcggcg cgtgggcgaa caaagaacag gccattgtga tgaatgttca
    24721 gcgccagccc ggtgctaaca ttatctccac cgccgacagc attcggcaga tgctgccaca
    24781 gctcactgag agtctgccga aatcggtgaa ggtgacagtg ctttccgatc gcaccaccaa
    24841 tatccgcgca tccgtcgatg atactcagtt tgaattgatg atggctatcg cgctggtagt
    24901 catgattatc tacctgtttt tgcgcaatat tccggcgacc atcattcccg gtgttgctgt
    24961 accgctgtcg ttaatcggca ctttcgcggt tatggtgttt ctcgattttt caatcaataa
    25021 cctgacactg atggcgttaa ctatcgccac cggattcgtg gtcgatgacg ccatcgtggt
    25081 gatcgaaaac atttcccgct atatcgaaaa aggcgaaaaa ccgttggcgg cggcgctcaa
    25141 gggcgcaggt gaaatcggct ttaccattat ctcgctgatc ttctcactga ttgcggtgtt
    25201 gatcccactg ctgtttatgg gcgatatcgt cgggcgactg ttccgcgaat ttgctattac
    25261 cctggcggta gcgattttga tctcagcggt ggtgtcgctg accctgacac cgatgatgtg
    25321 cgcgcggatg ctcagccagg agtcgttgcg taaacagaac cgcttctccc gtgcctcgga
    25381 aaaaatgttc gacaggataa tcgccgccta tggtcgtgga ctggcgaaag tgctgaatca
    25441 tccgtggctg accttaagcg tggcactcag cacgctgctg cttagcgtgc tgctgtgggt
    25501 gttcattccg aaaggtttct tcccggtaca ggacaatggc attattcagg gcactttgca
    25561 ggcaccgcaa tccagctcct ttgccaatat ggcccagcga caacgccagg tcgcggacgt
    25621 gattttgcag gatccggcag tgcaaagcct gacctcattt gttggcgttg atggcactaa
    25681 cccgtcgctg aatagtgcac gtttacaaat caacctcaaa ccgttggatg aacgtgatga
    25741 tcgggtgcaa aaagtcatcg cccgtctgca aacggcggta gataaagtgc cgggcgtcga
    25801 tctcttcctg caaccaacgc aggatctgac tattgatact caggtcagcc gcacccagta
    25861 ccagtttacc ttgcaggcca cctcactgga tgcgctcagt acctgggtgc cacagttgat
    25921 ggaaaaactc cagcaactgc cacagctttc tgatgtctcc agcgactggc aggacaaagg
    25981 gctggtggcg tatgtcaatg ttgatcgcga cagcgccagc cgtctgggga tcagcatggc
    26041 ggatgtcgat aatgccctgt acagcgcgtt tggtcagcga ctgatttcca ctatttatac
    26101 tcaggctaac cagtatcgcg tagtgctgga gcacaacacc gaaattaccc cgggcctcgc
    26161 gacgctggat accattcgcc tgaccagtag cgacggcggc gtggtgccgt taagctcaat
    26221 tgccaaagtt gaacagcgtt ttgcgccgct ttccatcaac catctggatc agttcccggt
    26281 aacgaccatc tcctttaacg tgccggataa ctattcgctg ggcgatgcgg tgcaggcgat
    26341 tatggacacc gaaaagacgc tgaatctgcc ggtggatatc accacgcaat tccagggcag
    26401 caccctcgcc ttccagtcgg cgctgggcag cactgtctgg ctgattgtcg cggcggtggt
    26461 ggcgatgtat atcgtgctcg gcattctgta cgagagcttt attcacccga tcaccattct
    26521 ctcgacgcta cccaccgcag gggtcggcgc gctgctggca ttaatgattg ccggtagcga
    26581 actggatgtg attgcgatta tcggcattat tttgctgatc ggtattgtga agaagaacgc
    26641 catcatgatg atcgacttcg cgctggctgc tgagcgcgag caaggcatgt cgccgcgcga
    26701 tgcaatctac caggcttgtc tgttgcgttt tcgtccgatc ctgatgacca ccctggcggc
    26761 tctgcttggc gcgctgccgc tgatgttgag taccggggtc ggcgcggaac tgcgtcgtcc
    26821 gttaggtatc ggcatggtcg gcggtctgat tgtcagccag gtgctgacgc tgttcaccac
    26881 gccggtgatt tatctgctgt tcgaccgcct ggcattgtgg accaaaagtc gctttgctcg
    26941 ccatgaagag gaagcgtaag tgaagttttt tgccctcttc atttaccgtc cggtggcgac
    27001 gattttactg tcggttgcca ttaccctgtg cggcatactg ggcttccgta tgctgccggt
    27061 cgccccgctg ccgcaggtcg attttccggt gattatggtc agcgcctcgc tgcccggtgc
    27121 gtcaccagaa acaatggcgt cttccgttgc cacgccgctg gagcgctcac ttgggcgcat
    27181 tgccggagtc agtgaaatga cctccagcag ttcgctcggc agcacgcgta ttattttgca
    27241 gtttgatttt gaccgggata tcaacggcgc ggcgcgcgat gtgcaggcgg cgatcaacgc
    27301 tgcacaaagt ttgctgccca gcgggatgcc cagccgtccg acctatcgca aagctaaccc
    27361 gtcggatgcg ccaattatga tcctcacgct gacatccgat acttattcgc agggtaaact
    27421 gtacgatttc gcctcgacgc agctggctcc gacgatttcg caaatcgacg gtgttggtga
    27481 tgtcgatgtc ggcggcagct cactgcccgc cgtacgcgtc gggctgaatc cgcaggcgct
    27541 gtttaatcag ggcgtctcgc tggacgacgt acgcactgcc atcagcaatg ccaacgtgcg
    27601 taaaccgcag ggggcgctgg aagatggcac tcaccgctgg cagatccaga ccaatgatga
    27661 gctaaaaacc gccgccgaat atcagccgct gattattcat tacaacaacg gcggcgcggt
    27721 tcgtctgggc gatgtggcga cagtgaccga ctctgtacag gatgtacgta acgccgggat
    27781 gaccaatgcc aaaccggcta ttttgctgat gatccgcaag ctgccggaag ccaatattat
    27841 ccagacggtt gacagcatcc gggcaaaatt accggagttg caggaaacca ttccggcggc
    27901 gattgatctg caaattgctc aggatcgcgc ccccaccatt cgcgcctcgc tggaagaagt
    27961 cgagcaaacg ctgattatct cggtggcgct ggtgattctg gtggtgtttt tattcctgcg
    28021 ctcgggtcgc gcaactatta ttcccgccgt tgcggtgccg gtttcgctga ttggtacgtt
    28081 tgcggcgatg tacctgtgcg gtttcagtct caataatctt tcgttaatgg cgctcaccat
    28141 cgctactggt ttcgtggtgg atgacgccat cgtggtgctg gaaaacattg cccgtcatct
    28201 ggaagcggga atgaaaccgt tgcaagccgc actgcaaggt actcgcgaag tcggttttac
    28261 ggtgctgtcg atgagtctgt cactggtggc ggtgttcctg ccgctgctgt tgatgggcgg
    28321 attgccgggc cgactgttac gcgaatttgc cgtgacgctt tctgtcgcca ttggtatatc
    28381 gttgctggtt tctctgacat taacgccaat gatgtgtggc tggatgctga aagccagcaa
    28441 gccgcgcgag caaaagcgac tgcgtggttt tggtcgcatg ttggtagccc tgcaacaagg
    28501 ctacggcaag tcactgaaat gggtgctcaa tcatacccgt ctggtgggcg tggtgctgct
    28561 tggcaccatt gcgctgaata tctggctgta tatctcgatc ccgaaaacct tcttcccgga
    28621 gcaggacact ggcgtgttga tgggcgggat tcaggcggat cagagtattt cgtttcaggc
    28681 gatgcgcggt aagttgcagg atttcatgaa aattatccgt gacgatccgg cagtggataa
    28741 tgtcaccggc tttacaggcg gttcgcgagt gaacagcggg atgatgttta tcaccctcaa
    28801 gccacgcgac gaacgcagcg aaacggcgca gcaaattatc gaccgtctgc gcgtaaaact
    28861 ggcgaaagaa ccgggggcga atctgttcct gatggcggta caggatattc gcgttggtgg
    28921 gcgtcagtcg aacgccagct accagtacac gttgttatcc gacgacctgg cggcactgcg
    28981 agaatgggag ccgaaaatcc gcaaaaaact ggcgacgttg ccggaactgg cggacgtgaa
    29041 ctccgatcag caggataacg gcgcggagat gaatctggtt tacgaccgcg acaccatggc
    29101 acggctggga atcgacgtac aagccgccaa cagtctgtta aataacgcct tcggtcagcg
    29161 gcaaatctcg accatttacc agccgatgaa ccagtataaa gtggtgatgg aagtggatcc
    29221 gcgctatacc caggacatca gtgcgctgga aaaaatgttc gttatcaata acgaaggcaa
    29281 agcgatcccg ctgtcgtatt tcgctaaatg gcaaccggcg aatgccccac tatcggtgaa
    29341 tcatcaggga ttatcggcgg cctcgaccat ttcgtttaac ctgccgaccg gaaaatcgct
    29401 ctcggacgcc agtgcggcga tcgatcgcgc aatgacccag cttggggtgc cttcgacggt
    29461 gcgcggcagt tttgccggca cggcgcaggt gttccaggag acgatgaact cgcaggtgat
    29521 cctgattatc gccgccatcg ccacggtgta tatcgtgctg ggtatccttt acgagagtta
    29581 cgtacatccg ctgacgattc tctccaccct gccctcggcg ggcgttggag cgctgttggc
    29641 gctggagctg ttcaatgccc cgttcagcct aatcgccctg atagggatca tgctattaat
    29701 cggcatcgtg aagaaaaacg ccattatgat ggtcgatttt gcgcttgaag cccaacggca
    29761 cggtaacctg acgccgcagg aagctatttt ccaggcctgt ctgctgcgtt ttcgcccgat
    29821 tatgatgacc accctggcgg cgctgtttgg tgcgctgccg ctggtattgt cgggcggcga
    29881 cggctcggag ctgcggcaac ccctggggat caccattgtc ggcggactgg taatgagcca
    29941 gctccttacg ctgtatacca cgccggtggt gtatctcttt ttcgaccgtc tgcggctgcg
    30001 tttttcgcgt aaacctaaac aaacggtaac cgagtaaatg acagatcttc ccgacagcac
    30061 ccgttggcaa ttgtggattg tggctttcgg cttctttatg cagtcgctgg acaccaccat
    30121 cgtaaacacc gcccttccct caatggcgca aagcctcggg gaaagtccgt tgcatatgca
    30181 catggtcatt gtctcttatg tgctgaccgt ggcggtgatg ctgcccgcca gcggctggct
    30241 ggcggacaaa gtcggcgtgc gcaatatttt ctttaccgcc atcgtgctgt ttactctcgg
    30301 ttcactgttt tgcgcgcttt ccggcacgct gaacgaactg ttgctggcac gcgcgttaca
    30361 gggcgttggc ggcgcgatga tggtgccggt cggcagattg acggtgatga aaatcgtacc
    30421 gcgcgagcaa tatatggctg cgatgacctt tgtcacgtta cccggtcagg tcggtccgct
    30481 gctcggtccg gcgctcggcg gtctgctggt ggagtacgcg tcgtggcact ggatcttttt
    30541 gatcaacatt ccggtgggga ttatcggtgc gatcgccaca ttgctgttaa tgccgaacta
    30601 caccatgcag acgtggcgct ttgatctctc cggattttta ttgctggcgg ttggcatggc
    30661 ggtattaacc cctggcgctg gacggcagta aaggtacagg tttatcgccg ctggcgattg
    30721 ctggcctggt cgcagttggc gtggtggcac tggtgcttta tctgctgcac gccagaaata
    30781 acaaccgtgc cctgttcagt ctgaaactgt tccgtactcg taccttttcg ctgggcctgg
    30841 cggggagctt tgccggacgt attggcagtg gcatgttgcc ctttatgaca ccggttttcc
    30901 tgcaaattgg cctcggtttc tcgccgtttc atgccggact gatgatgatc ccgatggtgc
    30961 ttggcagcat gggaatgaag cgaattgtgg tacaggtggt gaatcgcttt ggttatcgtc
    31021 gggtactggt agcgaccacg ctgggtctgt cgctggtcac cctgttgttt atgactaccg
    31081 ccctgctggg ctggtactac gttttgccgt tcgtcctgtt tttacaaggg atggtcaact
    31141 cgacgcgttt ctcctccatg aacaccctga cgctgaaaga tctcccggac aatctggcga
    31201 gcagcggcaa cagcctgctg tcgatgatta tgcaattgtc gatgagtatc ggcgtcacta
    31261 tcgccgggct gttgctggga ctttttggtt cacagcatgt cagcgtcgac agcggcacca
    31321 cacaaaccgt ctttatgtac acctggctta gcatggcgtt gatcatcgcc cttccggcgt
    31381 tcatctttgc cagagtgccg aacgatacgc atcaaaatgt agctatttcg cggcgaaaaa
    31441 ggagcgcgca atgaagttct ggcgacccgg tattaccggc aaactgtttc tggcgatttt
    31501 cgccacctgc attgtcttgc tgatcagtat gcactgggcg gtgcgtatca gttttgagcg
    31561 tggctttatt gattacatca agcatggtaa tgaacagcga ttacaactgt taagtgatgc
    31621 gcttggcgag cagtatgcgc agcatggcaa ctggcgcttc ctgcgcaaca atgatcgctt
    31681 tgtctttcag atcctgcgtt catttgaaca cgataattcg gaagataaac ccggcccggg
    31741 tatgccaccg cacggctggc gtacccagtt ctgggtggtt gatcaaaaca acaaagtgct
    31801 ggttggtccg cgagcgccga ttccacctga cggtacacgg cgacccattc tggtcaacgg
    31861 tgcggaagtt ggcgcggtga tcgcctcccc cgttgagcgg ttaacgcgca atactgatat
    31921 caatttcgat aaacaacagc ggcaaaccag ctggttgatt gtcgccctgg caacgttact
    31981 cgcggcactt gccacttttc tgctggcgcg cggtttactg gcaccggtaa aacgacttgt
    32041 cgatggcacg cacaaactgg cggcgggcga tttcactacc cgcgtaacgc ccaccagtga
    32101 agatgaactg ggcaaactgg cgcaagactt caaccagctt gccagcacac tggagaaaaa
    32161 ccagcaaatg cggcgcgatt ttatggccga tatttctcac gaactgcgta cgccattagc
    32221 ggtgctgcgc ggtgaactgg aagccattca ggatggcgtg cgtaaattca cgccggagac
    32281 ggtggcgtct ttacaggcgg aggtcggtac actgaccaaa ctggttgacg atctccatca
    32341 gttgtcgatg tctgatgaag gcgctctcgc ctatcaaaaa gcaccggtag atttgatccc
    32401 actgctggaa gtggcgggcg gcgcatttcg cgaacgattc gccagtcgtg gcctgaaact
    32461 gcaattttcc ctgccagaca gtattaccgt atttggcgat cgcgaccgtt taatgcagtt
    32521 attcaataat ttacttgaaa acagcctgcg ctatactgac agcggcggca gcctgaaaat
    32581 ctctgccgag cagcacgaca aaacggtgcg cctgaccttt gccgacagtg cgccaggtgt
    32641 cagtgacgag cagctacaaa aattatttga acgtttttat cgcaccgaag gttcccgcaa
    32701 ccgcgccagc ggtggttccg ggctggggct ggcgatttgc ctgaacattg ttgaagcaca
    32761 taatggccgc attattgctg cccattcgcc ttttggcggg gtaagcatta cagtagagtt
    32821 accgctggaa cgggatttac agagagaagt atgaccgagt taccaatcga cgaaaacaca
    32881 ccacgtattt tgatcgtgga agatgaaccg aagctggggc agttgctcat tgattatctg
    32941 cgtgctgcga gctatgcgcc gacgcttatc agccacggcg atcaggtact gccgtatgtg
    33001 cgccagacac caccggatct gatcctgttg gatctgatgc tccctggcac cgatggcttg
    33061 acgctgtgcc gggaaattcg tcgtttttct gacattccta tcgtgatggt gacggcaaaa
    33121 atcgaagaga tcgaccgcct gctcggactg gagattggcg cagatgacta catctgcaag
    33181 ccgtacagcc cacgggaagt ggtagcgcgc gtcaaaacca ttttgcgccg ttgcaaaccg
    33241 cagcgcgaat tgcagcaaca ggatgccgaa agcccgttga ttatcgacga aggtcgtttt
    33301 caggcttcat ggcgcggtaa aatgcttgac ctgacacctg cggaatttcg tctgttgaaa
    33361 acgctctctc atgaaccagg aaaagtgttc tcccgcgagc aattgctcaa tcatctttat
    33421 gacgactacc gcgtagtaac cgaccgcacc attgacagcc acattaaaaa cctgcgccgc
    33481 aagctggaat ctctcgacgc cgaacagtca tttatccgcg ccgtttacgg cgttggttac
    33541 cgctgggaag ccgacgcctg ccgcatcgtt tagttttagc gacatgactt tgttagccgg
    33601 agatggcgtt cagaccagat ccggcaacat tatcccacgc atggtcagca aactgacatt
    33661 atctctccgc cctggcaggc atcagaaaat cgctcatact ttaatcggta aacagcacct
    33721 ttagatgctg ttttcgatac gcaatttcaa tcaaggagtc attatggctg gttggtttga
    33781 actcagtaag agcagtgata atcagttccg gtttgtgcta aaagcgggca atggtgagac
    33841 tatcctcacc agcgagctgt atacatcaaa agcctctgcg gaaaagggca tcgcgtcagt
    33901 gcgtagcaac agcccgcaag aagaacgcta tgagaaaaaa acggcaagta acggcaaatt
    33961 ctatttcaat ctgaaagccg ccaatcatca aattatcggc tccagccaga tgtacgccac
    34021 cgcgcaatct cgtgaaaccg gaattgcctc cgttaaagcc aatggcacaa gccagacggt
    34081 gaaagacaat acgtaattac gatgccgggc gtgggttgtt ggcgtccggc tttattttct
    34141 ctcatcacag agttactatc cctcaagaaa ttattaacca tgaaatgagt tttctcctca
    34201 taactgataa ggaaaattat gctcaaagga ttgcgtacat tattttcacg cagggttttg
    34261 tttaacgtcg aaatgaataa ttctgttgac tgtgaattac ttctcatcca gcaacaactc
    34321 caggcattga tagccgaacc aggcaattat ttagcacatg attgtgatct caacgatact
    34381 ctggttcttt gccagcttac ccatcatcac aatggatata tacgagaaaa ggccgtgcag
    34441 tatctggggc acaaagagga tgttgcggct atagaagaat tattacttgc agctaatgac
    34501 tgggtaaaag aggttcgcct tgctgcaacc atagcattac gtcatttaat gaaggataag
    34561 aatgcagcgg catttgccgt taatctaccc atgatacgta accttctcca atgccagcgt
    34621 tacgatcacc gtgcatttgt agacgagata cttagatatt tattgacaga aaataaccgc
    34681 ccacaattaa cgtattggct tacttgccct gataaaaagc tctcccgcgc ggtattacaa
    34741 acgctgattg aatacggtta ctttaatgat gaatcttcac tgctttcaat actcgataaa
    34801 cccgatgagg gtttgcgaat gctggcgacc acccactggc tgcgtcaaca attaccatta
    34861 agcgaagcgg tattaactcg tctgttaaaa gaccgctggc cgcgtattcg acaagcaacg
    34921 ttatttagcc tcactgaccg agcaatagaa atgcctccgg cgttgcacag cacattactg
    34981 ctggataaca atatgttgat ccgcctacgc gctaaaaata tgcttcacga agtcatggat
    35041 gttccgcaat tctggcgtca tgttgtcact tctgcggaat atactccctc acagcgacgg
    35101 gccgctttat acgggctgga ttccattcat gatcccaata ttttaaaact ggcagaatgg
    35161 ggattatcac agaatgtatt cccactgcgt ttagccgcta tgcacattct tgcaaaagct
    35221 aatcctcgtt gtggcgtcaa agagactatt ctgaccacgc tggctaaccc ggacgccgcc
    35281 gggttgcgat ttatggttaa catctgtgta tggtgtcgcg ttccgctcac gttcgaggaa
    35341 atacgtcagt tgcaggaaaa cgcgccgtca gttaaacatg cttgcgcata ttgtcggctg
    35401 tatcataact tgaacaaatg ggacggttta attcttcttc ttcaatcgca acacaagcta
    35461 actgaagaat ttgcaggtaa acagctcgcg atatggcaac gcaatttcaa tctttcgggc
    35521 attcaaccga atgctcttca gcgtcagcaa cttcaggcgc tttttacccg caatccggaa
    35581 cttcacaaca gattatgggg ttacattcct tttaaataac ttcgaattat agacttacga
    35641 tgacaaatgt acctgctgac gagcggaacc aaaacgaaac aatacgtgaa gctttacgct
    35701 tgctacgaga aaaacttgcc gagggactac gcagtggcga agcccaaccg tgggaaaagg
    35761 atctgttttt acataaagtg aaggccggta acaaataaat cccccagtct tttccctcac
    35821 atttgcactg gagccaaccc cgccgattgc gctacaatgc ccgcccttaa agtgggggca
    35881 ctcccctaac cgcttcatca ggtgaagcgg atctgacctg tcatcagaac gagagaatta
    35941 tgtttaaacc ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg
    36001 cttatggcgc agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca
    36061 acgaattcaa ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa
    36121 agttttatgt cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg
    36181 acctgaaacc ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga
    36241 ttatgctggt gcgtgagtac ttccctgaaa tgccgatcca cctctcggtg caggctaacg
    36301 ccgtaaactg ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct
    36361 ctcgcgaact gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga
    36421 tcgagatctt cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg
    36481 gctatatcaa caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt
    36541 acaacgtcca ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga
    36601 tcccggtgca aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta
    36661 tgatcgaaga agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca
    36721 cttacatcat gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa
    36781 tgggcgtgca ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca
    36841 ccgcacaggt ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca
    36901 gcctgctgga aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc
    36961 gtcatactca cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc
    37021 agtttgttgg tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa
    37081 ataaattctc cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta
    37141 cccttgagca catggaaaac gccaaaggtg tagcgatgcc ggtcgcacca ggcgatggtt
    37201 atactgtgtg gctcccggtc ccgcaggatc ttgaactaaa ttacgcgctg ctgatgcgta
    37261 atttctccgg ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc
    37321 ccggatggaa aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc
    37381 gtttcgctga aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc
    37441 tgtgtaatct cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt
    37501 cttccattga ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc
    37561 atcgtgctcg cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca
    37621 aaaaatattc tcactctcca catttgaatg tcagacgagc gacaccatgt aatcctacac
    37681 cttctgtctt cagctcaact atttgcattt ttttgccctg agtaacacag aaatgagttg
    37741 catcattttt tactatattt tctgcaccag atattctact cctggctaaa gaagcttcgg
    37801 cttcggtgta gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa
    37861 atttaccgat acttgtctca tcattatgca cagcacaacc agacataata aaaatactaa
    37921 ttaatgatat agcagctatc tttttcatct cacctttccc cattaaatac caacgacact
    37981 ctctagtgtt taaatataat aatggctgat cattatattt gaataggatt ataataaatg
    38041 ttctgtacaa catttcctac ataagtagga cttacagaca tgaaggccct ttccaggtaa
    38101 ctccatgggg ctttaatata tttggaatag tatatttaag atgccactgt ttggttgaat
    38161 attaggcata tgctcttttt tgaaatttat cggtggctac cgttaatatt cgctgttccc
    38221 attgcaagct cctggtggta accactgaat cctccatact tgaactgact ttttatcctc
    38281 cgactttcat cctgttctga ctccaccttt tcttttctgc tctacactat ctacagacca
    38341 atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggc
    38401 aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg
    38461 acgatccatg tgcgggtcac ctgggagaaa ggtgatgccg cacgatttgt agaggaggcc
    38521 cggaagttgg gcgtcgcaac ggtaattgcc ggtggtggcg atggcaccat taatgaagtt
    38581 tctacggcgt tgattcagtg tgagggggat ggcatacccg cgctgggaat tttgccatta
    38641 ggaaccgcca atgattttgc caccagtgta gggattcctg aggcactgga taaggcgctg
    38701 aaactggcaa ttgccggtaa cgccattgcg atagatatgg cgcaggtcaa caaacaaacc
    38761 tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga aacgccggaa
    38821 aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggat
    38881 actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc
    38941 ctggtattgg tattggtaac gggcgtcagg ccggtggcgg tcagcaattg tgtccgaacg
    39001 cgttaattaa cgatggcttg ctgcaactgc gcatttttac cggcgatgaa attcttccgg
    39061 ctctcgtatc aaccttaaaa tctgacgaag ataacccgag tattatcgaa ggcgcttcgt
    39121 cgtggtttga tatacaagcc ccacacgaaa tcacttttaa tcttgatggc gaaccgttga
    39181 gcggacaaaa ctttcatatt gaaatacttc cggcggcgtt gcgttgtcga ttaccaccag
    39241 attgtccatt attgcgttaa tcaaactatt ctctcttgaa ccagggagaa tccgaaatgc
    39301 cggatgcggt gtaaatacct tatccggcat acagaacaat actacaccgt aattaatttt
    39361 acccgtgtct tctgcaatgc cagtttatcc acctctgata cagcatcatc ggtaataata
    39421 atgtcaattt tttcgattgg cagtacctga ttaaaaccgc gacggttaaa tttcgatgaa
    39481 tcgagaacgg caatcacttt attagcggct gtgaccatcg caccgctaac ggaataaccc
    39541 tcattaaagg tcgtaatacc attaaccgca tcaataccat cagcaccgac aaacattaaa
    39601 tcggcattaa tatcctgcag ggaacgttca gcaatagatc catgcataga tcgcgtttta
    39661 tggcggaccg tgccaccgca gacgaccaga gtaatgtctt tattttcgga aagggcaaac
    39721 gctgccggga gactgttggt aatcaccgtg atatttttag cggtcattaa tccttcggcg
    39781 ataagcattg tggtacttcc gctgtcgaga ataacggtca tcccttcatg gatcattttg
    39841 actgccgcct gcgctattct gttttttggc gcgctggcga gctgaaatcg ctctttaaac
    39901 ccgacttcct gggtctcggt ttcactatta ccggacatta ttttcgccgc accgccatga
    39961 aagcgcgtaa ccacgccttt ttgttcgaga aagcgcaaat cggcacggat tgtcgcttcc
    40021 gaggcagcaa atactcccgc cagatcctga acaagcacgg ttccctgttc attcactaat
    40081 tggatgatct tattccttcg ctcgaatgaa ttcatcgtca tttacccata ttcaattgtg
    40141 gctagtgtaa acgaaggggg accattggtg aatgatctgt tttgcacaat accgggccag
    40201 catcacgctg gcccgcggtt tcatggaatg agcaacactt tgcccggcat ggcattacga
    40261 gcgatgtcac gcaccgcctg ggtgaagctt tcaaagcttc cacggtgagc gattaatggc
    40321 tccaggctta acttacgttc tgtcagcaac cggctcgccg tttcccactc ctgccccggc
    40381 caagggctgg aatagttcat ccaactgccg ataaccgtca gctctttacg caatattttg
    40441 ccaaacgttg ccgatgttaa atgcagatcc tgatgcaacg tgcccaccag cgccagttgg
    40501 gcatgaggac cggcaatctc taccgccagt tcgacagttt gcggtacgcc agccgtctcg
    40561 aggataagct gattaaaacg cagttcgcgt aaaacgctct gcatttgcgg cgcgctcatt
    40621 tcgctactgt taaatgtttg catcgaaccg aaagattttg ccagtgccag tttttctgag
    40681 ctaatgtcga tagccgtcac actctttgct cccagcgcga cagcgcactg aatagccagc
    40741 aggccaatgg ttccggcacc aataataata acgtttttat tctcacaacc ttgcgctaaa
    40801 tgaaaagcat gcagaccaat ggtaatcggc tcaataaaag ccccatcctc aataggcata
    40861 tccgtgggta gagcaaagac attttttcgc ttaacgacaa tatattcagc aaatccacca
    40921 tcacgccgcg agccaataaa atcatatttt gcgcactggg aataaaaccc tttcagacac
    40981 tctggacaag taaaacaggg taataacggc acacaggcaa ccgcatcgcc agggtgtaaa
    41041 tcatcaacac cggatcccac cgcatcaata tagccgctaa attcatggcc taacgttatt
    41101 ggataataat gtgcaccatt tttaaatatc ctgggtaaat cggaaccaca taagcccgag
    41161 ctggcaattt ttacccgcac ctcatcctga tgtttaattt caggaatgac gctttctgca
    41221 acgcgcacga taccatcagt atcattcacc actgatttca taaaaactcc tgattgttaa
    41281 gggggataaa ctccccctca gttaaaaatt attctgcgag aacggctttc tcttgtttaa
    41341 taaaaccacg cgctctacgc caggtaatga aaataccggt cagataaatt gcgccgataa
    41401 taatgaaacc gggaatattt tgcggggaga aaacctgaat cagtaaccag gtaatgggag
    41461 aaccgccctg atccattgaa gccaccatac ccccggcttt taacgcccca gcattagccg
    41521 ccagttgggt gtgcaaacca atagtttgcg tcgcgatcca cagagtgatg ctcatgatga
    41581 tgacacccga gattaaggtg cggaacagat ttccacgatg cacggcgact gccatcgcca
    41641 caaagaagcc gatggtggca agatcgccaa acggcagtac ctgattaccc ggaacacaaa
    41701 cagcaattaa aatggtgagc gggataaaaa tcaggcttac cgataccacc gccgtatgcc
    41761 ccagcagtaa tgcgggatcc aagccaatca ggaactcctg accgccgaac ttcgcctgta
    41821 aacgactacg ggcctgctta gcgatgggcg ttaaaccatc catgatgggt ttaatcaccc
    41881 gtggcattag cagcatcact gccgccgttt ttaccgccag ctgcaataca cctttgacat
    41941 cgtaacccgc gaggatgccg ataatcagcc ccatcacaaa acccacggtg acaggttcgc
    42001 caaaagggcc aaatttgcgc tgaatatcat cggcgctaaa tttaattcgg ttaacgcctg
    42061 ggattttctc gatgatagca tcgaccagca ccgcaatcgg ccccatatac gccgacgtac
    42121 cgtgcggaat agcaatacct tccagctcaa agaaatttcg ggtatcgcgg gcaaaccagt
    42181 cgccgagctt ataaacaaac gccgcgtgaa ttaccacacc cgccatccct atcatccatg
    42241 aaccggttgc cagatgcagc aacgcgccgg tgaaagtcat atgccagata ttccagatat
    42301 caatatttac cacccgcgtc atacgggtca gtaacatcgc cacgttaacc agaatagcaa
    42361 tcggaatcgc caccagcgca atttgcgacg cccaggtcat tggtgaagag cccggccagc
    42421 cgacatcgac cacatgcaga ttcaggtcga aattttccgc catcgctttc gccgccggac
    42481 caatggaatc cagcattaag ccaatcacaa ggccaatgcc aacaaagcca atccctatat
    42541 gaagacccgc tttaaagcaa tcgcctgcct tcatgcctaa tattttagaa aaaataatga
    42601 tgacaatcgg cagcatcacc gtagggccga ggtcgagaat ataacgcatg acttctgaaa
    42661 acataggtca cccctgtaag atagtcagaa ttttattttg taatgcttcg ataccgacac
    42721 cagaaacaaa aggcatgccg tgaactaacg gaatatcgcc aaaactacga tccaccctgg
    42781 cagtagtgca tatcaaatgc acgccatcca tataggtttc tatttcatta acccgacact
    42841 ggattaattc aacaggaata ttatgattct gacacaattc tttaatttct tccgccgcca
    42901 tcgtagaggt cgcaaccgcg cctccgcaag cgacaataat cttgcgtttc ataaatagtc
    42961 ctttttattg ttacagaggg atttattcgt ctgaatctaa aacatacttt gtgaagtatt
    43021 cctttaactg ggtttcagga agagtgatta gtgtctcgac gatatcgggc tgttgtaact
    43081 tgccaaataa acagcgtaaa agtttcaatt gctgctgcgg attttccaca atcaacgcaa
    43141 taaccaacga tacctccacg tcgttatcat catccgcttg ctgaaaatga actttatttg
    43201 ttggccttaa cagataaatg gctgacgact tagcatgaat cgcctcacaa tgcggtattg
    43261 caatggcgtg ctgctcaagc attatcccgg tagggaattc tgcttctctg gcaattaatg
    43321 cctgtggcca ggtatcatga accacacctt tggcgagcat ctcattaccg atatgggtta
    43381 aaacttcgct acgatcgaca aaagaaattc cgctacgaac aaacaggtta gtcatatacc
    43441 gtccttattc cgcacagccg tagcgatagg cgcgcaaaac atcataaatt ttatccataa
    43501 tcagctgatg cggtattgct gataattccc cggactgaat gcgttcaaat tgtttgggaa
    43561 gatactgact aatcatgccc agtgggatgt ccacgccttc aagattcacc atcatcgttt
    43621 cgacgctatt tttaatccga ctatgcggcc agtaataacg aatacgatcc gacaggctgt
    43681 aacgaatatc cagtaatgaa tcgttaaaac ccgtacgata atattttttc cagtattgcg
    43741 gttcgtcgag catcacttct tcaattaccg ccaggcaacc gctgcgattt tcaggggcga
    43801 taagttcctg ctcaatttgt gccagtgcaa atatcgcctc gcgtaaagca aaggttaatg
    43861 cgggaccgac tttcaatatt gcaaagtgat cgcggactaa ttcccaataa gccgtccggg
    43921 tctggtaatc ggtagaatgt gcttcataaa ccattcgggt gttttctatc cattgcgcca
    43981 gcggctgcgc ttcctgcggc tgataatgga taatattgct gtgatcaaat tccacgcccg
    44041 gctgcaccac gatggcaatc acgcgtgtta acgcctctgt cagcccacgg acaataaagg
    44101 ccttttgatg cgtacgtaaa gtattggcgg catcttcaac atgggtgatg tgtactgact
    44161 gaatggcgct ggcctcaccg cccggaaccg gaacttcggt gccaatgaca tagctcagtt
    44221 gctcacgctg gcaatctgtc gccacacttt ccgcagcaaa gcaaagcaca gcagctcgtt
    44281 ccgcaaccgt ttctggtgct aacggtatgg gatcccccgc gcaggacatt gacgcatcaa
    44341 gatgaatttt actgaagccg gcacgaacat attcctttac cagctcgacg gatttttcca
    44401 tcgccgcatc cgcattttct tgctgccagc agtttggccc cagatgatcg ccgccgagaa
    44461 taatgcgttc gcgtgcaaac ccaactttat cggcaatcgt aaaaacaaat tcgcgaaagt
    44521 ctgccggtgt cattccggta taaccgccaa attgattgac ctggtttgac gttgcttcaa
    44581 tcagcacttt gcgcgtgctg ttgcgatcaa atgccagcgc cgcttcgata accaacggat
    44641 gggcagaaca gactgaacat atgccgatat gttcaccagc tttatgccgg gcaattaacg
    44701 ttttcatgtt ttttccttat taaatggcag gtgcgttatg ccctgccctc gcagccacaa
    44761 tcggcaatca ctttgctcac cacatcgcgc attgcggatt tagccgactg caaataatcc
    44821 cggggctcgg tcgcttcagg gtgctcggtc aggtaatttt ttaacgcctg cgagaaggca
    44881 tttttcagct ccgttgcaac gttgattttg catatcccca gtttgatggt ttgctgaata
    44941 tccttagtcg ataaccctga cgcgccatgc agcaccagcg gtaagttcac ccactggcga
    45001 atgttctcca gtctagaaaa atcaagcgcc ggtgcgctgg catacatccc atgagccgtg
    45061 ccgatggcga ccgccaggga atcaattccg gttgcctcgg caaattcacg cgcctgagcg
    45121 gggttggtat aaaaagcatc ggcttcattg acttgcacat catcttcctg gccgccaagt
    45181 tgccccagct ccgcttcgac gctgacatca aagcgatggc aaaaatccac cacctctttg
    45241 acccgtgaaa tattttgcgc aaaaggcaaa tgcgaggcgt caatcatgac tgagcgcacg
    45301 ccagaacgaa ccttctgagc gatatcgtca aatttcgtgt gatggtcgag atgaattgcc
    45361 agtggatggt gatattgctt cgccatcgcg ctgaccagcg ccaacagatt ttctgtacca
    45421 gcatgagtaa atgtgccagg cgttccggcg atgatgaccg gcgcatgcag gttggcagcg
    45481 gtttccacca ccacttgcat cgtttcgaga ttgtgaatat tgaatgccgg aaccgcataa
    45541 ccgccgcgct gtgcgttgtt cagcatctgc tttgtcgata ccacgtgcat tttcatatcc
    45601 tgtcgtttgt tttcgttttc aaaatataat gaaattattt gtttttaaat atcgagataa
    45661 cgatcacaaa aacgacaata tgaaaattat tcgaggagtg aaaggcaaaa aaacggcctc
    45721 ccgataggga agccgtagca aagtgcgcgt gtttttatgc cggatgcggt gtaaacatac
    45781 gtggcagtgc tatgtaggca tgataagacg cttccgcgtc gcatcaggca taggttgccg
    45841 gatgcagtgt aaacgcctta tccgtcctac ggggtggtgt tgttcgtata aggcgtatca
    45901 ggcaatagta attttgctat cgagataaac gtcctgcacg gcgttaatca gtttcacgcc
    45961 gtcagccatc gatttcttga acgccttacg tccaagaatc agccccattc cgccagcgcg
    46021 tttgttgata accgcagtac gcactgcatc actgaggtca gtttcaccgc ctgcagcacc
    46081 gccggagttt atcaacccag cccggcccat atagcagtta gctaactgat aacgcaccag
    46141 atcaatcggg ttttcgctgg ttaacttgct gtacacgcga tcgtcggtat aaccgtaatt
    46201 aatcgcttta tagccgccgt tattttccgc cattttttgt ttgacgatat ctgcgccgat
    46261 ggttgccgcc agatggtttg cctgaccggt caggtcggcg gaaacatggt aatcaacgcc
    46321 atctttctta aaggcggagt tacgcaaata ggcccacagc acggtcacca tgcccagctc
    46381 atgcgcacgt tcaaaagccg cagaaatttc ttcaatctgg cgacgtgact cttccgagcc
    46441 aaaatagata gtcgcaccaa ccgccaccgc gcccatgttg aaagcctgct ccacgctggc
    46501 atacagcgtt tgatcgtagg tattcgggta acttagcgtc tcgttgtgat taagtttgac
    46561 gagaaatgga atgcgatgcg catagcgccg cgataccgac gccaacacgc cgtaagttga
    46621 taccacacag ttacagcccg cttcgatcgc cagttcaaca atgtttttcg ggtcaaagta
    46681 gagcgggtta gcagcaaatg aagctccggc agagtgctca acgccctggt caaccggcag
    46741 aatagaaaga tatcctgtgc cagccagacg cccggtgttg tacaacgtct gcatattacg
    46801 taacaccgct ggcgggcgat tattgtcaat cattacgcgg tctacgtagt catgtccggg
    46861 gagataaagc tggtcagaag gaattgtcat acaacggtgc tgtaaaaggt tgtcggcgtc
    46921 tttgccaagc aactgcgcaa tatctgtcat tactatgctc ccgtaaattc cgattggata
    46981 tcggctatgg attgtcctgg cccgcctttt gcgggcaatc ataatcctgg tcgttacggg
    47041 cgagtttttc catcttttgg atgttttttc agcgttttct gttggctcga ttcatcagaa
    47101 aaaatgttag cgcggtcaag ttttcaccgt aaaggcattt aaaaggtatt attaagtggt
    47161 attgtcatcg cgtaccttac attacctgtc atgaaggaat taaaagatga aaacaacagc
    47221 aaagctgtcg ttcatgatgt ttgttgaatg gtttatctgg ggcgcgtggt ttgtgccatt
    47281 gtggttgtgg ttaagtaaaa gcggttttag tgccggagaa attggctggt cgtatgcctg
    47341 taccgccatt gcggcgatcc tgtcgccaat tctggttggc tccatcactg accgcttttt
    47401 ctcggcgcaa aaagtgctgg cggtattgat gttcgcaggc gcgctgctga tgtatttcgc
    47461 tgcgcaacag accacttttg ccgggttctt tccgttactg ctggcttact ctctaaccta
    47521 tatgccgacc attgcgctga ctaacagcat cgcttttgcc aacgtgccgg atgtggagcg
    47581 tgatttcccg cgcattcgtg tgatgggcac tatcggctgg attgcctccg gtctggcatg
    47641 tggtttcttg ccgcaaatac tggggtatgc cgatatctca ccgactaaca tcccgctgct
    47701 gattaccgcc ggaagttctg ctctgctcgg tgtgtttgcg tttttcctgc ccggcacgcc
    47761 accaaaaagc accggcaaaa tggatattaa agtcatgctc ggcctggatg cgctgatcct
    47821 gctgcgcgat aaaaacttcc tcgtcttttt cttctgttca ttcctgtttg cgatgccact
    47881 ggcgttctat tacatctttg ccaacggtta tctgaccgaa gtcggcatga aaaatgccac
    47941 cggctggatg acgctcggcc agttctctga aatattcttt atgctggcat taccgttttt
    48001 cactaaacgc tttggtatca aaaaggtatt attgcttggt ctggtcaccg ctgcgatccg
    48061 ctatggcttc tttatttacg gtagtgcgga tgaatatttc acctacgcgt tactgttcct
    48121 cggtattttg cttcacggcg taagttacga tttttactac gttaccgctt acatctatgt
    48181 cgataaaaaa gcccccgtgc atatgcgtac cgccgcgcag gggctgatca cgctctgctg
    48241 ccagggcttc ggcagtttgc tcggctatcg tcttggcggt gtgatgatgg aaaagatgtt
    48301 cgcttatcag gaaccggtaa acggactgac tttcaactgg tccgggatgt ggacttttgg
    48361 cgcggtgatg attgccatta tcgccgtgct gttcatgatt tttttctgcg aatccgacaa
    48421 cgaaattacg gctatcaagg tcgatgatcg cgatattgcg ttgacacaag gggaagttaa
    48481 atgaaaacag aacgtattct cggtgctctt tatgggcagg cgttagggga tgcgatgggg
    48541 atgccctccg agctttggcc acgcagccgc gttaaagcac actttggctg gattgaccgt
    48601 tttctgcctg gaccaaagga gaataacgcg gcctgttatt ttaaccgcgc cgaattcacc
    48661 gacgatacct cgatggcgct gtgtctggcg gatgcgttgc tggaacgtaa aggcaagatc
    48721 gatccggatc tgattgggcg taatatcctc gactgggcgc tgcgtttcga cgccttcaac
    48781 aaaaacgtgc taggtccgac ctcgaagatt gcgcttaacg ccattcgcga cggtaaaccc
    48841 gttgctgaac tggaaaacaa cggcgtaacc aacggcgcag cgatgcgcgt ctcgccatta
    48901 ggttgtttgc ttccggcgcg cgatgttgat tcctttattg atgatgtggc gctggcgtcg
    48961 agcccgaccc ataaatccga tctggcagtt gcaggtgcgg tagtcatcgc atgggcgatt
    49021 tctcgtgcta ttgacggaga aagctggtca gcgattgttg attcactgcc ttcaattacg
    49081 cgacatgccc agcaaaaacg caccaccacg ttcagcgcct cactggcggc gcggctggag
    49141 attgcgctga aaattgtgcg caatgccgac ggcaccgaat ccgccagcga acagctttac
    49201 caggtcgttg gcgcaggtac cagcactatt gagtccgttc cgtgcgccat tgcgctggtt
    49261 gaactggcac aaaccgaccc gaaccgttgc gccgtcctct gcgctaacct tggcggcgat
    49321 acagacacca tcggtgctat ggcgacggca atctgcggcg cgttgcatgg cattaacgct
    49381 atcgatcctg cgttaaaggc ggagctggat gcggtaaatc agcttgattt caaccgctat
    49441 gccacagcgc tggcgaaata tcgtcaacaa cgggaggcgg tatgagcggc gctcgattac
    49501 acacgctgct gcctgaatta accacgcgtc agtcggtgat ggtcgtcggc gcggcggtca
    49561 ttgacgtgat cgccgacgct tatgccctcc cctggcgtgg gtgcgatatc gaactgaaac
    49621 agcagagcgt taacgttggc ggctgcgcac tgaatattgc cgtggcgtta aagcgcctcg
    49681 gcatcgaagc gggtaatgcc ttgccgctcg gtcagggggt atgggcggag attattcgca
    49741 accggatggc gaaagagggg ttaatcagcc tgatcgataa cgccgaaggc gataacggct
    49801 ggtgtctggc gctggttgag ccggatggcg aacgtacttt tatgtcattc agcggtgtgg
    49861 aaaatcagtg gaatcgccag tggctggcgc gattaaccgt tgcgcctggc agcctgctct
    49921 atttttccgg ttatcaactg gcctcgccct gcggcgaatt gttagtggaa tggctggaag
    49981 agctgcaaga cgtgacgccg tttatcgatt ttggcccacg tattggcgat atcccggacg
    50041 cattactggc gcggatcatg gcctgtcgac ctttagtgtc actcaatcgt caagaggctg
    50101 agattgccgc cgaacgtttt gctttatccg cagagataac aacacttggc aagcaatggc
    50161 aggagaaatt tgccgcgccg ttgatcgttc gtctcgataa agaaggcgca tggtatttca
    50221 gcaacgacgc ttctggctgc attccggcat ttccaacgca agttgtcgac accattgggg
    50281 cgggcgacag tcatgccggt ggcgtacttg ccgggctggc ctctggtctg ccactggcgg
    50341 atgccgtatt actgggcaat gcagtggcgt catgggttgt cgggcatcgg ggcggtgatt
    50401 gtgcgccaac gcgcgaggaa ctactcctcg cacacaaaaa cgtatagatc gctgcgacag
    50461 tggctaatgc tgtactcaat aggccgttgc tgttggtcaa gcgccacttg cttgatcacc
    50521 agcaccggta ttttgctatc taactgaata tgtgactgaa actcggcatc cggcatccgg
    50581 gcgctaacgc gggaacgcgt acgttgtggg taaatgtgct ggctgcggaa atagtcataa
    50641 agtgaaatcc cgatggcatc aacatcatga attaaatgcg ccggaaccca cgattcctca
    50701 atcgataccg cttcttcatc gacatagcga atacgcttaa gcaaaaagac atcacttcct
    50761 gcttcgaccg ccagctgctg cgcgacttct tcgggacatt tcacgacacg cttgttaacc
    50821 catagcgtat cgggcttttt cccgcgcaat accacctgct gagaaaaacc tcgcgcttct
    50881 ttcaacgagt attcgaagat gttgttgatc tgcgtgccgt aaccgcgcga acgcgtcacc
    50941 acaccctctt cttccagcgc ctgcatcgcc ttgcgcaccg taatgcgcga aacgccggtt
    51001 aactgactta aatcacgctc accgggcaaa atattgccat gctccagcac cccgctgcgc
    51061 acggcatttt ttaccgtttc ggcaaactta atataaagcg gcgtgttatc cgccgctaaa
    51121 atacgctcgt tcagttgggc aatcagctgg gtatgcgctt gttccatttg tcttgttcct
    51181 gccggggagt ctggtgggag tatatcactg cttcttattt aaggcgtttc tttaatccca
    51241 tcaacgaatg cctgcaactc ctccaccgtg ccattaaata cattaaaatc caccggacca
    51301 ttgatgccat ctacctgtcc acggtcggaa tgctgccaga agcgccaggc catgccgtca
    51361 ttgtccggac gacgttgata atagtgagcc acccaccacg gatattcatt gaaatagccc
    51421 gccagattgg tgtgataaaa aacggctcct gagtaaataa tcggcttttt tcccgtactt
    51481 ttttcgacca tttttagcca ctgacttact cgtttgcgta attcttttgc cgataatttt
    51541 ccccgttctt ctacgtccag taccgcaggg aaatcacctt gtgagaaatc caccgtttgc
    51601 agaaataatc gtgcctgaac cggcgctgct accgacgggg agaaataatg atacgccccg
    51661 cgcagcaggc cattttcgcg gcttagttgc cagttacgcg aaaaatacgg gtccaccagc
    51721 ttttcgcctt ccgtcgcctt aataaaagca aactgtaagc ggataccgtt gtcgcgcatt
    51781 tttgccaccc gctgccagtc gatccgctcc tgccagcgag aaacatctat tccgtgaatg
    51841 gtgtaactgg caggaatgcg aatagcgaaa gatttaaccg ggcgataacc gtagaaatgg
    51901 atgtagtctg taatctgaac tgctgtcgaa tagaaaaaat tcaccttctg acgcggataa
    51961 attgcaacga tggaaattag cccaagagcg cataatagag aggtgagttt ttttcgactg
    52021 gtaattctta gctgcattgg tttcatccct gaatgtcagt gccaaaggct gacaataacc
    52081 aaagcagcta tagtacggtg ctcaccacca ggcgtggaag tggtgaaccg gaccaatacc
    52141 gtgaccaact tccagcgtgt cggcctgggc taatgctgat gaaagccagc tttttgcctc
    52201 ctgtacggtg tcagcccagt ttgtatggcg cgggcgtagt gcagccaacg ccgcagagag
    52261 cgtacaacca gtgccgtggg tgtttttggt cataatgcgc ggtgcggtaa accgttgttc
    52321 accctcgcgg gtaaacagcc agtccgggct ttgctcatca tccagatgac cacctttcat
    52381 tagcactgcg ccacagccca tcgccaacag cgatcgccct tgttccagca tttcctgttc
    52441 agtgcgcgcg tgtggcgcgt cgagcaaggc ggcagcttcg ggcaagtttg gcgttattaa
    52501 tgaaacctgt ggcaataatc gactgcgcag cgtagcaacc gccgaaggtg aaagcagcgg
    52561 gtcgccgctt tttgccagca taacggtgtc gagtaccacg tattggatct gataacgttg
    52621 caaccgttct gccaccgctt caacaatatc ggtttccgcc aacataccga ttttagtggt
    52681 gtcgattcgc acatcgctga acaccgaatc gagctgggcg gcgacaaaat cagactcaat
    52741 gcgatacacc gactgtacgc cacgggtatt ttgcgccacc agtgcagtaa taactgagca
    52801 accataagcg ccaagtgccg agaaggtttt aagatcggcc tgaatccccg caccaccact
    52861 cggatcagta ccggcaatcg tcagagcgtt aattcgtttc atgcctgcac ctcctgcgtc
    52921 aattgccaga gcgcatcaag gaaatgtgga acaaaactgc ctggcccctc gcttctggcg
    52981 actgcgcgtt ctccggcttg tttcatccag tgacaggcag atgcgacatt ttccagcgta
    53041 tcgcctggta acgcacagca ggcagcgaca accgccgata atgcacagcc agttcctacc
    53101 actttggtca ttaacggatc gccaccgtga ataccaatga tacgatgtcc atcggtaaca
    53161 taatccatct cgccagtgac cacgacgatt gcgccagttt cccgtgccag tgtttgtgca
    53221 gcgggtatcg cgttagctgc ggcgtcagtg gtatccactc cccgtccacc attagcaatg
    53281 ccagctaatg ccatgatttc cgaagcatta ccacgtatag cggttggctt atgaggcaga
    53341 agttccagac aaaaacggcg gcgataatcg agtgcaccaa ccgctactgg atcaagcgtc
    53401 cagggtgttt gagagctttt tgcttgctca acggcggcac acatcgactg tgcgcgtagc
    53461 tgcgtcagcg tgccaacgtt aatcagcaac gcactagcaa tagccgcaaa ctgactggcc
    53521 tcttcggttt cgataaccat cgctggcgat gcaccgagcg ccagcaaggt attggcagta
    53581 aaggtttgca ccacatcgtt ggtcatgcag tgcacaagag gggaatgttg gtgaaaaagg
    53641 tgtaacgcgt gcgcagattg cgctgaactc tgcaggtcga cttgcatagt ttgctcctgc
    53701 cataacgtga agaagcaatg acctggtggt ccgtgacttc cctacgctgg cattatccag
    53761 atcaggtaat acgggtattt ctcagccttc acaaagaagg gcaccccgag tcatttaaaa
    53821 caaaatcaaa agattgcgat taacgtcccg ttaatccctg ttctggatca tgccagcgga
    53881 aagccaaaga ctcaacccta aaaaatcctc tgtgtcccag tttgtgtctc acgttgtggc
    53941 ccacagataa atggcaggca caaaaaaacc gcccataagg cggcttaagt aaattattca
    54001 gtcgtgaaga tttaaatgaa atctattttc tgcatacgct ttcttcatgc agtcgaagtg
    54061 cttttgccaa ttccaattgg aatgcaaagg aaagacggga acggctgatc ttgataccgt
    54121 caataatgat gtatgtaggg taaacagtga ccatgcggcc caagtattgg aagtgtttag
    54181 ggtggtttac tttatcgtta ttcattgtgt gccatccgtc tgggtggggg taatcccggc
    54241 aacggatgat tgatctaaaa tcgatcaaaa tcaataactt aacagatcac caaatccagg
    54301 ggggtgtaca ttggcgagcc tccctcagcc taacctatac acgaccataa aataggggcg
    54361 gattccttcc agttgggtgg gaaaatatct gggagtttgg atacagtagc caggcagggt
    54421 aaccagaccc atagatgaga atgtttatta ttcccaaata tccaggcgag gggcctgctg
    54481 tggctctctt tacgcgccgc tgtggcgtct tctctcctct ttctatacaa tcccatagct
    54541 aacccattgc gaggcttaca gagcgttaca gagcgtccgt gtgaatattg cataaatgaa
    54601 agtggtgaaa ggtccgattt ggtcaacttt tagacaacta tttaacatat gcgcaacagg
    54661 ttagacctgt accctttcaa taggtccgcc tcctgggcgc tgtgtacgcc ccataactgt
    54721 ggctcttctg tacccatccg ggagccattc cacagagtaa actgtaaaac tgatctgcct
    54781 gtgagtaacg cccttttcct gtggataact tccacgcact aaccccataa actccccttt
    54841 tgaggcgtcc gcctcttcag gccctttctt gtggcaaaaa cacaaaaagg gtttagggcg
    54901 gtggctaccg cccctgtaga tgggaatgag tcgcactcga aagagggatt gagaaccaat
    54961 tctcaagtga taatcgatct catttgtgtt cgaagttttc cccagaaaaa taattttggg
    55021 gttaagcatg taaaatgcta cacacttgca ctttgtaatg ataattaatt gaccataaaa
    55081 acaaatagtt agaagcgctg ggggagtccc actagttaca caattgattg ctttttgagc
    55141 taaaactgat tcttttaccg ggtatacaat ccatttgatc gccctttacc acatatttaa
    55201 acggatttaa agccatttat ttagggggtt agcaaagtgt tgattccatg gttacagata
    55261 acaaaaaagc ccgccggggg ttagccgggg gcctgtggtt gggggtgtca aacgtggtta
    55321 gttcggcccg ccctgcatta gcatgttgaa agcctgctga ttattgctct caacttcaga
    55381 gcgtaccaga tccttaacct atccatcctg cactttcaca ttaacgttca cgtttagcgg
    55441 ctggtttagc cacgctggcg gcgtcatcag tggattacct gccatatcaa ctttaagctg
    55501 tggcgctgta atcgtcggcg taggaggtac ataagttgga ttggagtaag agcttaccca
    55561 ccctgtttta tctgctggtt tggcaaagtc tggattagtc ggaaggtctg gactatggag
    55621 gaaatcccac agtttaccca gcgggctatc ttttttcaga ttgcccccgg cggtggtgtc
    55681 ctgtccatac atggcgtcgt tagcgtcatg accttatccc aggcataatc cccacctacc
    55741 gaaccgaccg caatgctcca gccaacagga ccgccagaga gagccgccgc gccgcctacc
    55801 ttgccaactt tcggaccccg acccggtttg tttggcttgt ctttaccttc cccaccagat
    55861 ccgccagtag tagcggcttc aatgccacca atagcggcga tccctttcat gacccccagt
    55921 aatttggtta gtgggttaag catggtcaac aaccatttaa aggctacgcc caacttccag
    55981 atagacccgg cgaacatagc caccccggcg gcgtatgctg ccatttcgcc ccaggcttta
    56041 agctgctcac cagtgatccc catcttgctg gcgtaatact caataatgcg gaaggtcagg
    56101 atcacagtgt catgcaactc tgtaaacgca tctgtggcac cctcaacgat tttcccggca
    56161 atatgcccga acatctcaaa ggccccacca gagccgttaa cagcggcgct gatagcgtcg
    56221 aaagcgctgg ttaattcgtt gccgaagcca gaagcaaaga agatgttcat cgcgttctgc
    56281 atactggttt gcaggcgctg ccagcttgcc cggttggact tcatagcctt atccagagcg
    56341 ccgccgttac gagctgcggc tttcatctgt ttagccactt ccgggaggat gtccttagag
    56401 aacagcttcc cttgttgcat caggtcaaac agatcctttt ctgtaaggtt ggttgtccct
    56461 ttcatctggt tgagagcctt aacgaacaga cccacagccc ccgggagagc ttcggacaac
    56521 tggcctttga gttcttcact cattaccgtt cctttagaca tcatctgatt caaacttttc
    56581 aaagcgcggc tggcttcatc ggctgaagca cctgtaacaa ggccccattc actggcaccc
    56641 tcaaagatgg atcgcatctg gtctttaggg agcttgtcac caacagcggc ggcaaactga
    56701 gtgtaatcac gggaagtctt gaggagatcc agccccaaac ggttggattg atcacggaga
    56761 taatcaaact ctttagcggc cttatctgtg gaaccggtga tcgctgtcag accagaaagc
    56821 gcggattcaa aatcctgtcc ggtggtcatg atcttgccgc tcagcgccac agttgcggca
    56881 atggtgatcg ccccgcctgc accctcgatc atgg
//
LOCUS       DAJTSL010000007        47219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTSL010000007 DAJTSL010000000
VERSION     DAJTSL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.007"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            17..754
                     /locus_tag="OO433_000382"
     CDS             17..754
                     /locus_tag="OO433_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6048986.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGKASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYDGLSAKKEQNLP"
     gene            865..1875
                     /gene="pyrD"
                     /locus_tag="OO433_000383"
     CDS             865..1875
                     /gene="pyrD"
                     /locus_tag="OO433_000383"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6048987.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            2049..2591
                     /gene="zapC"
                     /locus_tag="OO433_000384"
     CDS             2049..2591
                     /gene="zapC"
                     /locus_tag="OO433_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6048988.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNVLVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(2588..3697)
                     /gene="ycbX"
                     /locus_tag="OO433_000385"
     CDS             complement(2588..3697)
                     /gene="ycbX"
                     /locus_tag="OO433_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6048989.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     ILEGEVSPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            3941..6049
                     /gene="rlmKL"
                     /locus_tag="OO433_000386"
     CDS             3941..6049
                     /gene="rlmKL"
                     /locus_tag="OO433_000386"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6048990.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGALSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            6061..7968
                     /locus_tag="OO433_000387"
     CDS             6061..7968
                     /locus_tag="OO433_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6048991.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIVLIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNIAEGKQEVMVNGKPRHVLSYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            8098..9351
                     /gene="pqiA"
                     /locus_tag="OO433_000388"
     CDS             8098..9351
                     /gene="pqiA"
                     /locus_tag="OO433_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6048992.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            9356..10996
                     /gene="pqiB"
                     /locus_tag="OO433_000389"
     CDS             9356..10996
                     /gene="pqiB"
                     /locus_tag="OO433_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6048993.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENTDVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            10993..11556
                     /gene="pqiC"
                     /locus_tag="OO433_000390"
     CDS             10993..11556
                     /gene="pqiC"
                     /locus_tag="OO433_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6048994.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSVQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            11812..11979
                     /gene="rmf"
                     /locus_tag="OO433_000391"
     CDS             11812..11979
                     /gene="rmf"
                     /locus_tag="OO433_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6048995.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(12049..12567)
                     /gene="fabA"
                     /locus_tag="OO433_000392"
     CDS             complement(12049..12567)
                     /gene="fabA"
                     /locus_tag="OO433_000392"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6048996.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(12636..14396)
                     /locus_tag="OO433_000393"
     CDS             complement(12636..14396)
                     /locus_tag="OO433_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6048997.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPNLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            14582..15034
                     /gene="matP"
                     /locus_tag="OO433_000394"
     CDS             14582..15034
                     /gene="matP"
                     /locus_tag="OO433_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6048998.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKLDLQALLGKE"
     gene            complement(15110..16150)
                     /gene="ompA"
                     /locus_tag="OO433_000395"
     CDS             complement(15110..16150)
                     /gene="ompA"
                     /locus_tag="OO433_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6048999.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(16507..17016)
                     /gene="sulA"
                     /locus_tag="OO433_000396"
     CDS             complement(16507..17016)
                     /gene="sulA"
                     /locus_tag="OO433_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6049000.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            17235..17864
                     /gene="sxy"
                     /locus_tag="OO433_000397"
     CDS             17235..17864
                     /gene="sxy"
                     /locus_tag="OO433_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6049001.1"
                     /translation="MKSLSYKRIYKSQEYLAMLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(17827..19989)
                     /gene="yccS"
                     /locus_tag="OO433_000398"
     CDS             complement(17827..19989)
                     /gene="yccS"
                     /locus_tag="OO433_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6049002.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGFWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(19999..20445)
                     /locus_tag="OO433_000399"
     CDS             complement(19999..20445)
                     /locus_tag="OO433_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6049003.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            20568..22628
                     /gene="helD"
                     /locus_tag="OO433_000400"
     CDS             20568..22628
                     /gene="helD"
                     /locus_tag="OO433_000400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6049004.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPLPVNRLEEFDNCREAWRK
                     CQAWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLA
                     GAGSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITART
                     FHALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEE
                     MQWSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIK
                     LMAPLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAA
                     LLAALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNS
                     RIGKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERIL
                     ILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAAR
                     ESIMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPV
                     ARKP"
     gene            complement(22660..23118)
                     /gene="mgsA"
                     /locus_tag="OO433_000401"
     CDS             complement(22660..23118)
                     /gene="mgsA"
                     /locus_tag="OO433_000401"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6049005.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVAANVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(23214..23876)
                     /locus_tag="OO433_000402"
     CDS             complement(23214..23876)
                     /locus_tag="OO433_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6049006.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            24049..24462
                     /locus_tag="OO433_000403"
     CDS             24049..24462
                     /locus_tag="OO433_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6049007.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(24507..24824)
                     /gene="hspQ"
                     /locus_tag="OO433_000404"
     CDS             complement(24507..24824)
                     /gene="hspQ"
                     /locus_tag="OO433_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6049008.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(24882..26072)
                     /gene="rlmI"
                     /locus_tag="OO433_000405"
     CDS             complement(24882..26072)
                     /gene="rlmI"
                     /locus_tag="OO433_000405"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6049009.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            26167..26445
                     /gene="yccX"
                     /locus_tag="OO433_000406"
     CDS             26167..26445
                     /gene="yccX"
                     /locus_tag="OO433_000406"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6049010.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(26442..26771)
                     /gene="tusE"
                     /locus_tag="OO433_000407"
     CDS             complement(26442..26771)
                     /gene="tusE"
                     /locus_tag="OO433_000407"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6049011.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(26862..27521)
                     /gene="yccA"
                     /locus_tag="OO433_000408"
     CDS             complement(26862..27521)
                     /gene="yccA"
                     /locus_tag="OO433_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6049012.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(27728..27815)
                     /locus_tag="OO433_000409"
     tRNA            complement(27728..27815)
                     /locus_tag="OO433_000409"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27779..27781),aa:Ser,seq:tga)
     gene            28242..29360
                     /gene="hyaA"
                     /locus_tag="OO433_000410"
     CDS             28242..29360
                     /gene="hyaA"
                     /locus_tag="OO433_000410"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6049013.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            29357..31150
                     /gene="hyaB"
                     /locus_tag="OO433_000411"
     CDS             29357..31150
                     /gene="hyaB"
                     /locus_tag="OO433_000411"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6049014.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMINLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            31169..31876
                     /gene="hyaC"
                     /locus_tag="OO433_000412"
     CDS             31169..31876
                     /gene="hyaC"
                     /locus_tag="OO433_000412"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6049015.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDGTVISTMV
                     NGYRSHKFGKISNKECS"
     gene            31873..32460
                     /locus_tag="OO433_000413"
     CDS             31873..32460
                     /locus_tag="OO433_000413"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6049016.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
                     QLANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            32457..32855
                     /gene="hyaE"
                     /locus_tag="OO433_000414"
     CDS             32457..32855
                     /gene="hyaE"
                     /locus_tag="OO433_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6049017.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPTTLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            32852..33709
                     /locus_tag="OO433_000415"
     CDS             32852..33709
                     /locus_tag="OO433_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6049018.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            33843..35387
                     /gene="appC"
                     /locus_tag="OO433_000416"
     CDS             33843..35387
                     /gene="appC"
                     /locus_tag="OO433_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6049019.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWAFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            35399..36535
                     /gene="appB"
                     /locus_tag="OO433_000417"
     CDS             35399..36535
                     /gene="appB"
                     /locus_tag="OO433_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6049020.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLSSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            36548..36640
                     /gene="cbdX"
                     /locus_tag="OO433_000418"
     CDS             36548..36640
                     /gene="cbdX"
                     /locus_tag="OO433_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6049021.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            36720..38018
                     /gene="appA"
                     /locus_tag="OO433_000419"
     CDS             36720..38018
                     /gene="appA"
                     /locus_tag="OO433_000419"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6049022.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYFGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNV
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(38133..40313)
                     /gene="etk"
                     /locus_tag="OO433_000420"
     CDS             complement(38133..40313)
                     /gene="etk"
                     /locus_tag="OO433_000420"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR6049023.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLHDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(40333..40779)
                     /gene="etp"
                     /locus_tag="OO433_000421"
                     /gene_synonym="yccY"
     CDS             complement(40333..40779)
                     /gene="etp"
                     /locus_tag="OO433_000421"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6049024.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(40767..41906)
                     /locus_tag="OO433_000422"
     CDS             complement(40767..41906)
                     /locus_tag="OO433_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6049025.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTRNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(41952..44048)
                     /locus_tag="OO433_000423"
     CDS             complement(41952..44048)
                     /locus_tag="OO433_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6049026.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVNDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(44048..44794)
                     /locus_tag="OO433_000424"
     CDS             complement(44048..44794)
                     /locus_tag="OO433_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6049027.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(44791..45435)
                     /gene="gfcB"
                     /locus_tag="OO433_000425"
     CDS             complement(44791..45435)
                     /gene="gfcB"
                     /locus_tag="OO433_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCR6049028.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(45542..45847)
                     /locus_tag="OO433_000426"
     CDS             complement(45542..45847)
                     /locus_tag="OO433_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HCR6049029.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(46289..46501)
                     /gene="cspH"
                     /locus_tag="OO433_000427"
     CDS             complement(46289..46501)
                     /gene="cspH"
                     /locus_tag="OO433_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6049030.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            46787..46999
                     /gene="cspG"
                     /locus_tag="OO433_000428"
     CDS             46787..46999
                     /gene="cspG"
                     /locus_tag="OO433_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR6049031.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            47010..47198
                     /locus_tag="OO433_000429"
     CDS             47010..47198
                     /locus_tag="OO433_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6049032.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVTLQIRTDHAPLDFTK"
BASE COUNT        11628 a        11729 c        12408 g        11454 t
ORIGIN      
        1 cagtcgggta aagtgaatgc cagcgcgaca tttgtacttc attatgacta atacgtggaa
       61 cagattggcg ctcttgattt tcgccgtttt atcgctgctg gtggcgggtg aattgcgggc
      121 aggagtggtg gtcggcggaa cgcgatttat ctttccggca gacagagaat cgatatctat
      181 tttactgact aataccagtc aggaatcctg gctgattaac agtaaaatca accgcccaac
      241 gcgttgggcc ggggggaaag cgtcgacagt gccagcacca ttactggccg ctccgccact
      301 tattctcctg aagcccggta ctacaggcac gttgcgcttg ctgagaacgg aaagcgacat
      361 cttgcctgtg gatcgcgaaa cgctatttga gttaagcatt gccagcgtgc catccggcaa
      421 agttgaaaat cagagcgtaa aagtggcgat gcgctcggta tttaaactgt tctggcgacc
      481 cgaaggtttg ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga cacggaattc
      541 gcagggtgta caactcacta acccaacgcc ttactacatt aacctgattc aggtgagcgt
      601 aaatggtaaa gcgttaagta atgcgggagt tgtgccgcct aaaagccagc gtcagacaag
      661 ctggtgtcag gcaatcgcac cgtgtcatgt cgcctggcgg gcgataaatg attacgacgg
      721 gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg ttcggaaaac gtttgcgttt
      781 tttttgccgc aggtcaattc ccttttggtc cgaactcgca cataatacgc ccccggtttg
      841 cacaccggga atccaggaga gttcatgtac taccccttcg ttcgtaaagc ccttttccag
      901 ctcgatccag agcgcgctca tgagtttact tttcagcaat tacgccgtat tacaggaacg
      961 ccgtttgaag cactggtgcg gcagaaagtg cctgcgaaac ctgttaactg catgggcctg
     1021 acgtttaaaa atccgcttgg tctggcagcc ggtcttgata aagacgggga gtgcattgac
     1081 gcgttaggcg cgatgggatt tggatcgatc gagatcggta ccgtcacgcc acgtccacag
     1141 ccaggtaatg acaagccgcg tctctttcgt ctggtagatg ccgaaggttt gatcaaccgt
     1201 atgggcttta ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa ggcccattat
     1261 gatggcgtcc tgggtattaa catcggcaaa aataaagata cgccagtgga gcagggcaaa
     1321 gatgactatc tgatttgtat ggaaaaaatc tatgcctatg cgggatatat cgccatcaat
     1381 atttcatcgc cgaatacccc aggattacgc acgctgcaat atggtgaagc gctggatgat
     1441 ctcttaaccg cgattaaaaa taagcaaaat gatttgcaag cgatgcacca taaatatgtg
     1501 ccgatcgcag tgaagatcgc gccggatctt tctgaagaag aattgatcca ggttgccgat
     1561 agtttagttc gccataatat tgatggcgtt attgcaacca ataccacact cgatcgttct
     1621 cttgttcagg gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg tccgcttcag
     1681 ttaaaaagca ccgaaattat tcgccgcttg tcactggaat taaacggtcg cttaccgatc
     1741 atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc tgcgggtgcc
     1801 tcactggtgc aaatttattc tggttttatt tttaaaggtc cgccgctgat taaagaaatc
     1861 gttacccata tctaattatt tcttcgaatt cctctttata agataatcag ggctttattt
     1921 tcagccctgg ttgttttata ttcatcgctg ttgcttattt agacattttg tacttttatt
     1981 gttgaggtta ttaagcgaag cgacaatgga ttgtgttgct gcggttttat agggtagggg
     2041 agaggcagat gcgaattaaa ccagacgata actggcgttg gtattacgat gaagagcacg
     2101 atcgaatgat gctcgattta gccaatggta tgctatttcg ctcacgtttt gcgcgcaaaa
     2161 tgttgactcc agatgccttt tctcccgcag gcttttgcgt tgacgacgcc gcgctctatt
     2221 tctcttttga agaaaagtgc cgcgatttta atttatctaa agaacaaaaa gccgaactgg
     2281 tgctgaatgt actggtagca attcgctatc tcaaaccgca aatgccgaaa agctggcatt
     2341 ttgtttccca tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc gtttggttga
     2401 gcgatactca cgagcaggtc aatttgctgg tcgttgaatc tggcgaaaat gccgcactat
     2461 gcctgctggc acaaccctgc gttgttattg cgggtcgcgc gatgcagttg ggcgacgcca
     2521 ttaaaatcat gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc agcctcgaac
     2581 aggcagtcta acgcgccaac ttaagtgcag tcttcggtac acagctacag caaagaatgg
     2641 tgccatcatc gcccattgct gattttttca gcggcgagac ttcgccttct aaaatctgaa
     2701 cacggcaact tccacaaatg cccgcgcggc aagaataagg gatacgaatt ccctgatttt
     2761 ctaattgctc cagcaacacc tgttggttat ttccacgaaa tgcctgtccc tgccagtcaa
     2821 tatctacatt tgcgtccggt tgttgcgtga tgttggcggt atcatcagcg gcagctgcgc
     2881 cgtaaatttt tgccggagcc gttgccagaa tttccacctc atcgccaacg cgaatcacgc
     2941 cgctattacg ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc tgggcagtgc
     3001 ggaaagattg caatgttttt aatggttcgc ctgccggatg tttttgccct ttttctgggc
     3061 tgacggtggt gaaaatacag cggctacaag gtttaaccac atcaaacacc acatcaccaa
     3121 tgcgaatcac tttccagcga tcttcttccc aggctgacgc gccggaaacc accagattgg
     3181 ggcggaattg ctccattttt acactggccg gacaacgttg ttggagatca cgtaacgagg
     3241 cttcgttagc aagaaggtaa ggatagccat cagcaaatga cagaggtaca gtgttgtggc
     3301 gtttcacgcg ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg cgggagaaaa
     3361 atccacttag ccatttgttg atcgcgtctg gcgcaattcg cgcggtaaaa tgtgtgcccc
     3421 aaacttcggt tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat gcgctactgc
     3481 catctggtgc ggtgagatgc aagccatcat gcacaggtga aggagtaaac cgtaccatct
     3541 ggggaaactg gcgagcggta ataaacgtac cgtcaggttc cgtgatcata aagatgcgat
     3601 cgaaggccag accactgaca tcagccagag catgtgtaag accaatgccg cgcatcgatt
     3661 taacaggatg aataaaaagc cgggttaatg tcgccacggt gcggtcctca aatgaaaata
     3721 agccctcaac tttatgacat gacgcgctta ttagctataa tgcgcaacaa ttttcttagc
     3781 gcctgtccca ttaggctatt ttatttgtca gtttggccct gggttgtgct cgaaatcctc
     3841 acgtacttgt gtacgctccg ttttctccgc gctgtccatg tccagactga ctgcaacaat
     3901 tacgcctact gcggtaggct cttagagtaa aagtgacgat atgaattctc tgtttgccag
     3961 tacggcccgt gggctggaag agctgttaaa aactgaactg gaaaacctgg gggccgttga
     4021 atgccaggtg gttcagggtg gggtccattt caagggcgac acacggcttg tttaccagag
     4081 cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt gtaaggttta
     4141 cagcgattta gacctctatc tcggtgttca ggcgatcaac tggacagaga tgtttaatcc
     4201 tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac accatccgca acagtcagta
     4261 cggcgcgatg aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa atctgccgcg
     4321 tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac gtctggctgc ataaagaaac
     4381 cgccagtatc gctcttgatc tgagtggtga tggtttacat ctgcgtggct atcgcgatcg
     4441 tgctggtatt gcgccgatca aagaaaccct ggcagccgct attgtgatgc gatccggctg
     4501 gcagcctgga acaccgctgc tcgatccgat gtgtggttcc ggtacgttgc tgattgaagc
     4561 ggcgatgctg gcgaccgatc gcgcacccgg cttgcaccgt gggcgttggg gctttagcgg
     4621 ctgggcgcag catgatgaag ctatctggca ggaagtgaaa gcggaagcgc aaactcgcgc
     4681 ccgtaaaggc ctagctgagt atagctctca tttttacggt tcggacagcg acgcacgggt
     4741 gattcaacgt gcacgcacta acgcccgtct tgcggggatt ggtgaactga tcacctttga
     4801 ggtgaaagat gtcgcgcaac tgaccaatcc gctgccgaaa gggccgtacg gcacagtgtt
     4861 gagcaacccg ccatacgggg aacgtctgga cagcgaaccg gcgctgattg cgctgcatag
     4921 cctgctgggt cggatcatga aaaaccagtt tggtggctgg aatctctctt tgtttagtgc
     4981 ctcgccggat ctgctaagct gcttgcagct gcgtgcagac aaacagtaca aggcgaaaaa
     5041 cggcccgctg gactgcgtac agaaaaatta ccacgttgcc gaaagcactc cagacagcaa
     5101 accggcgatg gtagcggaag actacgccaa ccgcctgcgt aagaacctca aaaaattcga
     5161 gaagtgggct cgccaggaag gaattgaatg ttaccgcctg tatgacgccg atctgccgga
     5221 atataacgtt gccgttgacc gttattccga ctgggtggtg gtgcaggagt atgcaccgcc
     5281 aaaaactatt gatgctcaca aagcgcgtca gcgtctgttc gatattatcg ctgcaaccat
     5341 ttcggtactg gggattgcgc caaacaaact ggtgctgaaa acccgtgaac gccagaaggg
     5401 caaaaaccag taccagaaac tgggcgagaa gggcgagttt cttgaagtta ccgaatataa
     5461 cgctcacttg tgggtgaacc tgacggatta tctcgatact ggtctgttcc tcgatcaccg
     5521 tatcgcccgc cgtatgctcg gtcagatgag caaaggcaaa gatttcctca acctgttctc
     5581 ttataccggt agcgccaccg tgcatgcggg attaggcggc gcgctcagca ccaccaccgt
     5641 ggatatgtcg cgtacttatc tggagtgggc agaacgcaac ctgcgtctga atggcctaac
     5701 cgggcgtgcg catcgcctga ttcaggccga ttgcctggcg tggctgcgtg aggcaaatga
     5761 acagtttgat ctgatcttta tcgatccgcc aaccttctct aactcaaaac gaatggaaga
     5821 tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa gatttgaaac gtctgctgcg
     5881 tgcaggtggg acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg atctcgacgg
     5941 cctggcaaaa ctgggactga aagcacaaga aattacgcaa aaaacgctct cccaggattt
     6001 cgcccgtaac cgccagatcc acaactgctg gctgattacc gcagcctgaa aggaatagta
     6061 atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc gcttctcgat
     6121 aacgcagaac tgcatatcga agataacgaa cgtgtttgtc tggtgggccg caacggcgcg
     6181 ggcaaatcga cgttaatgaa aatcctcaac cgtgaacaag ggctggatga cggtcgcatt
     6241 atttacgagc aagatttgat tgtagctcgt ctgcaacagg atccgccgcg taacgttgag
     6301 ggtagcgttt atgatttcgt tgccgaaggc attgaagaac aagcggaata tctgaaacgc
     6361 tatcacgata tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct caacgaactg
     6421 gcgaaggttc aggaacagtt ggatcaccac aacctgtggc agctggaaaa ccgcatcaac
     6481 gaagtgctgg cgcaactggg gttagatcct aatgttgcgc tgtcgtcgct ttccggcggc
     6541 tggttgcgta aagcggcatt aggacgcgcg ctggtgagta atccgcgcgt gctgttgctt
     6601 gatgaaccga caaaccacct ggatattgaa accatcgact ggctggaagg gttccttaaa
     6661 accttcaatg ggacgattat tttcatctcc cacgaccgtt cgtttatccg caatatggcg
     6721 acgcgcattg ttgatctcga tcgcggcaag ctggtgacct atccagggaa ttacgaccag
     6781 tacctgcttg aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc cgagttcgat
     6841 cgcaaactgg cgcaggaaga ggtgtggatc cgccagggga tcaaagcacg ccgtacccgt
     6901 aatgaaggcc gcgtacgcgc cctgaaagca atgcgtcgcg agcgtggtga acgtcgcgaa
     6961 gtgatgggta ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa gatcgttttc
     7021 gaaatggaag acgtttgcta ccaggttgac ggtaagcaac tggtgaaaga tttttcggcc
     7081 caggttctac gtggcgacaa aattgtcctg attggtccga atgggtgcgg caaaaccacg
     7141 ctgctgaaac tgatgctcgg tcagcttcaa gcggacagcg ggcgtattca cgttggcacc
     7201 aaactggaag tggcttattt cgatcagcac cgcgcggaac tggatcccga taaaacggtg
     7261 atggataaca ttgccgaagg taagcaagag gtgatggtta acggcaagcc acgccacgta
     7321 ttgagctatt tgcaggactt tctgttccat ccgaaacggg cgatgacgcc ggtacgtgcg
     7381 ctttctggcg gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa accaagcaac
     7441 ttattgattc ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga actgctggaa
     7501 gaactgatcg acagctatca gggcacggta ttgctggtta gccacgatcg tcagtttgtc
     7561 gataacaccg ttacagaatg ttggatcttc gaaggcggcg gtaaaattgg tcgttatgtc
     7621 ggcggttatc atgatgcccg tggtcagcaa gagcagtatg tggcgctcaa acagcctgcg
     7681 gtgaaaaaaa ccgaagaagc cgccgcggca aaagcagaaa ctgtaaaacg cagcagtagc
     7741 aaactaagct ataaattgca gcgcgaactg gagcagctac cgcaattgct cgaagatctg
     7801 gaggcgaagc tggaagccct acagacgcag gtggcggatg cttccttctt cagtcagcca
     7861 catgagcaga cacaaaaagt gctcgccgat atggctgctg cagagcagga gctggagcaa
     7921 gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc gcaaaatagt
     7981 cgatttaaac agagacagct gacaacggta aaattgcctg atgaactggg tttatcaggc
     8041 atgaaaacca acactttgtt agtcatctgt aaaatagcgc atcattaagg agtaccaatg
     8101 tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat gctggtggcg
     8161 ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac aacgttaacc
     8221 gtggcgtggg atgcccctcg gcagcgtccg accgcctatg cgttggctgc actgttcatg
     8281 ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt taccagtgaa
     8341 attacattac tggaaattcc cggcgtgctt ttttctgagg actacgccag cctcggcacc
     8401 tttttcctgt tgtttgtgca actggttccc gcgttttgtc tgataaccat tctgttactg
     8461 gtgaatcgcg cggaattacc ggtccgttta aaagagcaac tggcacgggt gctttttcaa
     8521 ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg tgctggtcag tttcgttaaa
     8581 ctgatggctt acggcagcat tggggtaggc agcagctttc tcccctggtg tttattttgt
     8641 gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga cgacatcgcc
     8701 ccgatgccag aactgcgcca gccgctaaaa ccaggcgtca cggggatacg tcaggggctg
     8761 cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg cccgcgttgt
     8821 agtaccaaag ggtacgttcg gcgtagaaac agcctgcagt ggacactcgc gctgcttgta
     8881 acgtccatca tgctgtatct tccggctaat attttgccca tcatggtgac ggatttatta
     8941 ggctcgaaga tgccgtcgac gattctcgct ggggtcattc tgttatggag cgaaggctct
     9001 tatcccgtcg ctgcggtgat ctttctggcc agtattatgg tgccaacgtt aaagatgatc
     9061 gccatcgcgt ggctgtgttg ggatgccaaa gggcatggca agcgcgacag tgaaagaatg
     9121 catttgattt atgaagttgt tgagtttgta ggccgctggt cgatgattga cgttttcgtt
     9181 atcgcggtgc tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta tccggcaatg
     9241 ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt tttctgctat gacgtttgac
     9301 ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg acagtatgga
     9361 atctaataat ggggaagcca aaatccagaa agtgaagaac tggtctcccg tgtggatatt
     9421 tcctatcgtc acggcgctca ttggggcctg ggttctattt tatcattaca gccatcaggg
     9481 accggaagtg accctgatca ccgcgaatgc ggaaggaatt gaaggtggca aaaccaccat
     9541 taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc acactggctg atgatttgac
     9601 gcacgttgaa atcaaagcgc ggctgaattc cggtatggaa aaattgctgc ataaagacac
     9661 cgtcttttgg gtggtgaaac cgcagattgg tcgcgaaggg attagcggcc tgggaacgct
     9721 gctgtctgga gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa tggataaata
     9781 cgatttgctg gactcgccac cgttggcccc gcctgatgcg aaaggtatcc gtgtgattct
     9841 cgatagcaaa aaagccgggc agctctcgcc aggagatccg gtgctgttcc gtggctatcg
     9901 ggtaggttcg gttgaaacca gcaccttcga tacgcaaaaa cgcaatatca gttatcaact
     9961 gttcatcaat gcaccttatg accgactggt gaccagcaat gttcgcttct ggaaagatag
    10021 tggcattgcg gttgatctga cgtcagcggg gatgcgtgtg gagatgggct cattgacaac
    10081 gctgcttagt ggcggtgtta gctttgatgt gccggaaggt ctggatttag ggcagccagt
    10141 ggcaccgaaa acagctttcg ttttgtatga tgatcagaag agcattcagg attcgttgta
    10201 caccgatcac attgattatc tgatgttctt taaagattcg gtacgcggtc tgcaaccggg
    10261 agctccggta gaattccggg gtattcgcct gggtaccgta agcaaagtgc cattctttgc
    10321 gccgaatatg cgtcagacat ttaacgatga ttaccgtatt ccggtactga ttcgtatcga
    10381 gccagagcgg ctgaaaatgc agcttggcga aaatacggat gttgttgagc accttggcga
    10441 attgttgaaa cgtggtttac gcggatcgct gaaaaccgga aacctggtca ctggtgcact
    10501 gtatgttgat ctcgatttct atccaaatac gcctgcaata accggtattc gtgaatttaa
    10561 tggttatcag attatcccga ctgttagcgg cggcctggcg caaatccagc aacgactgat
    10621 ggaagcgttg gataagatca acaaactgcc attgaatccg atgattgaac aggcaaccag
    10681 tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg atagcatgaa
    10741 caagatcctc gctagccagt cgatgcagca gttgccgacg gatatgcagt caacgttgcg
    10801 tgaattgaat cgcagcatgc agggcttcca gcctggctcc gcagcctaca acaagatggt
    10861 ggcggatatg cagcgccttg atcaggtgtt gcgagaactg caaccggtgc tgaaaacgct
    10921 caatgagaag agtaacgcgc tggtatttga agcgaaggac aaaaaagatc cagagccgaa
    10981 gagggcgaaa caatgaaaaa gtggctagtg acgattgcag cactgtggct ggccggatgc
    11041 agctccggcg aaattaataa aaactattac cagttacctg tggtgcagag cggtacacaa
    11101 agtaccgcca gccagggcaa tcgtctgtta tgggtagagc aggtcactgt tcctgactat
    11161 ctggcgggga atggtgtggt ttatcaaacc agtgatgtga agtatgtgat tgccaacaac
    11221 aacttgtggg ccagcccgtt ggatcaacag ttgcgcaaca ccctggttgc caacctgagt
    11281 acgcaactgc ccggctgggt ggttgcctcc cagcctctgg gaagcgtcca ggacacgctc
    11341 aatgttaccg taacggagtt taacggtcgc tatgatggca aggtcattgt cagtggtgag
    11401 tggctgttga accaccaggg acaactgatc aaacgtccgt tccgtctgga aggagtgcaa
    11461 actcaggatg gttacgatga gatggttaaa gtgctggccg gtgtctggag tcaggaagcc
    11521 gcttctattg cacaagagat aaagcgtcta ccttaattat aaagatttgt aagtataacc
    11581 gtctccggta tgttgcctga ggcggttttt ttgtttctaa cgtgcggaaa aatttgttcc
    11641 tcttcacatt ttttgtacaa ccgacatgcc cgtgtagctc acaaatatga cagtggcgtg
    11701 aattttgcgc attgacggca gttatgattc gcggtattgc ttaactgtga ttgcacattt
    11761 agtaatcact gttttctttt ccaccagaaa ccagtatgag ggaaacgagg catgaagaga
    11821 caaaaacgag atcgcctgga acgggcacat caacgtggtt atcaggccgg catcgccgga
    11881 cgctcaaaag aaatgtgtcc ctatcagacg ctgaatcaaa ggtcacaatg gctgggaggc
    11941 tggcgagaag ccatggcgga cagggtagta atggcctgat tctgtctctt taaaaagaaa
    12001 cctccgcatt gcggaggttt cgccttttga tactctgtct gatgataatc agaaggcaga
    12061 cgtatcctgg aacagaccga ctttcaggtc gctggcggta tagatcagac gaccatcaac
    12121 cagcacttcg ccatctgcca gacccataat cagacgacga ttaacaatgc gtttaaagtg
    12181 aatacggtag gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt tcacttcgcc
    12241 aacgcccagc gcgcggcctt taccttcgcc gcccagccag ccgaggtaga accctactag
    12301 ctgccacatt gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa tgaagtggca
    12361 tccgaagaac cacagatccg gattgatatc cagttctgct tcaacatacc ctttgtcgaa
    12421 gttaccaccc gtttcggtca ttttgaccac acggtccatc atcagcatgt tcggtgctgg
    12481 caattgcggg cctttagcgc caaacagttc accgcgacca gaggcaagaa ggtcttcttt
    12541 tgtataggat tcgcgtttat ctaccatgtt ctctgtaagc cttattttat tgaagcacgc
    12601 aggatagcta acacgtgtac gctgaacaag tccgatcagt tcggaataaa ccagttcagc
    12661 caacgtaatg gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg cgcgatacgt
    12721 tcctggatgg tttgcatcag cgtcgtttgg ccttcgccat cccacaccag atttaataat
    12781 aacggcagtg cgtcagtcac atcgtctact gcccagatgg tgaatttgcc ttcttctacc
    12841 gctttcacca gttcactgtg aagacttaaa tggcgaacgt tagctgtggg gataatgaca
    12901 ccttgtttcc cggttaactc acgttgctgg caaatagcaa agaagccttc gattttctca
    12961 tttaaaccac cgaccggctg ggcgcgaccg aactgatcga ctgaacctgt gatagcgata
    13021 ctctgattca ccggcacatc ggcgagggcg cttatcaggg cgcagagttc agccatcgag
    13081 gcactatctc catcaacttc actgtatgac tgctcaaatg tcagcgatgc tgagaagggg
    13141 atctgttgct caagctgtag ttccgacatc aggaacgctt gcatgatcat catccctttc
    13201 gcatggatat tgccgccaag ttccgctttg cgttcgatgt cggtgaattc accatcgcca
    13261 atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg acccggaaat
    13321 tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc aatcaggatt
    13381 tgctcctgaa ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg ccattcacgc
    13441 tgctgcagca ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca cagggaggcg
    13501 acctctttac actggcggag gatccactgc gggctaagcg gaagtgtttc ttgttcaccg
    13561 gtgtagcgtg ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg ggcaggtaag
    13621 tgattatgtc tggcggtaaa tgtcacccag cgacaccact ggcttactga ctccgcatcg
    13681 acaatctgca gagtatcttc aaattcgcta taaatagcct gctctgaaag ctctggctcc
    13741 atctcctgga aatcagccaa tgattcgcgt tcgcctacca gaatgacttt cagcttcaat
    13801 ggcatcgaag gcacagagac agggagaggg cgcgactcat caaacgcaac ccagtcaaaa
    13861 cgctcgcggt taacgatatt tttcagccgc atccacagca gaggttgcgc cagcagtgta
    13921 cgcaaagaga tgatgagaat accgccattt gcctgatgca ccagaccagg ctgcagggta
    13981 atgtcgccat taaactggcg caggcagcca aagagttgct ccgcttctac ccagtcggca
    14041 gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg acgtaagcgg
    14101 atggagtggc cggaaacctc atagtgatcg cccaccagtt ggcctgcatc gctttgtagc
    14161 gtacgcgcgg catcggcaat aagattcaga tactcagact cttccggggc cttagccagc
    14221 ataaaagagg aggatgctcg cgtatgcagc aactgctcca gcgcaaattg cagccgcggt
    14281 tgagtatcac tgaataaagg atcgttttcg tcaatcaaat ttggctgagc aaatatttcc
    14341 tgatagctat cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat ggtcaaagtt
    14401 gatgtttttt agtctgttgt caaagccgcg attataccgt aaccggcact acagcacacg
    14461 tagaaagcac cgacaatact cctggcatgg gcgttaaagc tcacaggatg gagattcttt
    14521 tcttcactgg cctaaaaagc tgatattctg taaagagtta cacggtaaca ttgagatcgc
    14581 tatgaaatat caacaacttg aaaatcttga aagcggttgg aaatggaagt atctggtgaa
    14641 gaagcatcgc gaaggggagt taattacccg ttacatagaa gccagtgccg cccaggaagc
    14701 cgtcgatgtg ttgctctcgc tggaaaatga acccgtgctg gtaaatggct ggattgacaa
    14761 gcatatgaat ccggaactgg tcaatcgcat gaagcaaacc attcgggcca gacggaagcg
    14821 ccattttaat gcggaacacc agcatacgcg caaaaagtcg atcgatctgg aatttattgt
    14881 ctggcaacgt ctggctggtc ttgcgcagcg tcgcggtaaa acgctttctg aaacgattgt
    14941 tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt cttctctgaa
    15001 gctggatctg caggcattgc tgggtaagga ataactgacg aaagtcagtt caatttacta
    15061 aaggcaaaaa aaaccccgca gcagcggggt ttttctacca gacgataact taagcctgcg
    15121 gctgagttac aacgtctttg atacctttaa cttcgatctc tacgcgacga tccggagcca
    15181 ggcagtcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca gtaaccgggt
    15241 tggattcgcc cataccacgt gcggagatct tgtctgccgg gatacctttg gagatcaggt
    15301 aatcaacaac agactgagca cggcgctcgg acagaccctg gttgtaagcg tcagaaccga
    15361 tgcggtcggt gtaacccaga acaactacgg aaccgtcttt cggatccagg ttgctcagct
    15421 ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct ttgttgaagt
    15481 tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt gccggagctg
    15541 gagccggagc aactactgga gctgcttcgc cctgaccgaa acggtaggaa acacccaagc
    15601 tcagcatgcc gttgtccgga cgagtgccga tggtgtgtgc gtcaccgatg ttgttggtcc
    15661 actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca ccgccagcga
    15721 agaccggaga aacgccggtg tcgtggtttt taccataaac gttggattta gtgtctgcac
    15781 gccataccat accacccaga cgagtgtaga tgtccaggtc gtcagtgatt gggtaaccca
    15841 gtttagcggt cagttgaacg ccctgagctt tgtatgcacc gttttcaacg ctgcctttgt
    15901 acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg ttaacctggt
    15961 aaccaccaaa agcaccagcg cccagttggt tttcatgggt cgggccattg ttgttgatga
    16021 aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag gtgttatctt
    16081 tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc gcgatagctg
    16141 tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat ctccaacgag
    16201 ataacacggt taaatccttc accgggggat ctgctcaata ttaactctac cgatatcttc
    16261 ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa acttacaagt
    16321 gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta atcttttttg
    16381 tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct ccgcaaggaa
    16441 aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga ttaatcctaa
    16501 atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc tcgtggcgtg
    16561 agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat ttgccgcatc
    16621 aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga tcaccacact
    16681 gtaattgccc gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag gggagagctg
    16741 gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt cccgactcag
    16801 tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt gctgtaacaa
    16861 tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa cttcactgat
    16921 aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg cggatgagaa
    16981 cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc ctgtgagtta
    17041 ctgtatgaat atacagtaca tccagtgaca acaaagatca accctatttt cggaaagagc
    17101 ctcgcaaatt ttttcgttgg tgacgggaaa acataaatta atcttgcccc ttaagaataa
    17161 gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta tgttatgatt
    17221 ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca caagaatacc
    17281 tggcaatgtt gggcacaatt gaataccgat cattgtttgg cagttacagc ctgaccgttg
    17341 acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct tgtgagcaaa
    17401 gtgcacagta ctgtgtaaaa catccgcctg tctggctgac atataaaaag tgtggccgat
    17461 ccgttaccct taattactat cgggttgatg aaagtctatg gcgaaatcaa ctgaagctgg
    17521 tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg cgcaataccc
    17581 gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt ctcggggagg
    17641 tggggattaa ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt tggttgcgac
    17701 tgcggcagca aaacagtctg gtgacagaaa agattctgtt tatgcttgaa ggtgccatta
    17761 tcggcattca tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca gaatgggctg
    17821 actcacttac gccgaaacag gagtttcctg cggaacttga gtaatctggc gttgcagacg
    17881 accaatctca ggcagtaatg caatcaataa tccaacttgt tgtacgacca gaggttcttt
    17941 gctgtctgcc cgtggttcaa gttgctgcat ccgctgtttc aggctagcta atgcctcatt
    18001 gacgcgttct tcatcagcag gttgatgatg taacgcgtca tcaacatagc aaactgcgtc
    18061 atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac cgagggctga
    18121 gatatagctg gtaaacgtat ggttaaggca cagcaaccga aacgcggctt cgcgaatttg
    18181 cggggtaacg ttcggctcgc tggacatatt tgataccacc gacgccagct cagcatcacg
    18241 gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc cctgatggta
    18301 ttgctccagt atggcatcga gataccgaca gttggcctct gtggcgcgtt cgagcatgcg
    18361 cggcaaattg cgaaactgcc agtcaggcca gatgtagctc actgccgccc acgcaatggc
    18421 acaaccaatc agcgtatcga ttacgcgagg taacgctact tcaaaacctt cacccagtaa
    18481 gttaaaacac agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt attgcacgtt
    18541 acggaaggca aaaaagagca cgccggtaat aaccagcagc accagctgcc cttccagtga
    18601 tggcacaaac cacagcacag gaatgccaat ggcgatacct accagcgtac caataatcct
    18661 taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca aacttgtcag
    18721 caagatccaa aacccgtgat gcattccggt tatttgaatg atggcgtagc cgaagcacaa
    18781 caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga agtgacggct
    18841 aagacgcagc cagatatcac tcaacccgtg cgggctgtca tcagcgagct cattttcgtc
    18901 attattatgg ggtagtgcct gggcctgttc tgattcaatt gttgccagtt gggcatcaat
    18961 ggcgcgtaaa ttgttcagca aaaatcccag tgttttgagt aaatcggcgg gtgcgccgtt
    19021 atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa aatgcggatc
    19081 atgttgataa ggctgacgca acaaaataca gcgtgacagt tgctggcacg cctggccctg
    19141 catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat gttcacgcaa
    19201 tgtttgatac tgaatatgag aagagctggc acgctcgtga atatcctgtg cgacaaagta
    19261 ataatgcagc gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc gggtcagcag
    19321 cgagagtttc gtctgattca atgtcgccat cagcagaccg ttggcgagag ccaaatcgta
    19381 cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgcgact tgagctcaag
    19441 ataacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc ggaccgggaa
    19501 cagcagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca gcagatacat
    19561 cggctgctga taccagtgct catacagtga tgttcccaac atagtgtaaa tggcgatcag
    19621 caatgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca aaatgaagcc
    19681 gctggtagag agcgttaagc caatcgcaaa tagccagggc cagggaaaca gcaattctac
    19741 tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca aacgtcccgc
    19801 cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta gcggaatcgt
    19861 cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg caataaaaat
    19921 acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag gacttagcat
    19981 aaaggccata ttagtggatt attcaaaacg acgacgtgca ttggcttcac gcgcagcttg
    20041 cgctgtttct accgatacca cgcgacgacc aaccggccat agtgcaatag cggcaatttt
    20101 aaagttcgca atgccgacag gaatgccaat gattgaaata cattgtgcga tgcccgttgc
    20161 aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat tcaataccgt
    20221 accgccagta ttcagcagca catttttgcc agccgggttc agttcatcga catgaatagc
    20281 ttcattgcca taaggcacca gagacagttt agtgatctcc cagcaggatc gtgtcagcgg
    20341 taaggtaaaa atcagcacaa tactgaccag agtcgccaac agccagccca gagtggtggc
    20401 aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac ctcgctttac
    20461 tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc acgttttctc
    20521 tgacggttac actgataaga aataatttcg tgtggatcta cagagtcatg gaactgaaag
    20581 cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg atcctcaatg
    20641 ccgggattaa agtctccggc gatcgccacg aataccttat tcctttcaat caattactgg
    20701 cgattcactg taagcgtggt ctggtatggg gcgagctgga atttgtactg ccggacgaaa
    20761 aagtggtgcg tctgcacggc accgaatggg gcgagacgca gcgtttttac catcatcttg
    20821 atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt gttttacgcc
    20881 agcaactgga tttgattgcc acgcgcactg gagaaaataa atggctgacg cgtgagcaaa
    20941 cctctggcgt tcagcaacaa atccgccagg ctttgtcggc gttgccgttg ccgttgccgg
    21001 ttaaccgact ggaagaattc gataactgcc gtgaggcgtg gcgtaaatgt caggcctggt
    21061 tgaaagatat tgaaagcgct cggttgcagc ataaccaggc gtataccgaa gccatgctta
    21121 ccgagtatgc ggattttttc cgccaggtcg agtcttcacc gctgaatccg gcgcaggccc
    21181 gggcagtcgt taatggcgag cattctctgt tagtgctggc aggtgcagga agcggaaaaa
    21241 cgtcggtgct ggtggcccgt gcaggctggt tgctggcacg tggtgaagcg tcccctgagc
    21301 aaattttatt gctggcgttt ggtcgcaaag ccgctgaaga gatggacgag cggattcgcg
    21361 aacggctaca taccgaagac attaccgcac gcacgtttca tgcgctggcg ctgcatatta
    21421 ttcagcaggg cagcaaaaaa gttccgatag tcagcaaact ggaaaatgat accgctgccc
    21481 gtcatgaact ctttattgct gagtggcgca agcaatgcag cgaaaagaaa gcgcaggcga
    21541 agggctggcg gcaatggctg acggaagaaa tgcagtggtc agtgccagaa ggtaacttct
    21601 gggatgatga aaaattacag cgtcgccttg cctctcgcct cgatcgctgg gtaagtctga
    21661 tgcggatgca cggtggtgca caggcagaaa tgattgccag tgcacccgaa gagattcgcg
    21721 atctgttcag taaacgtatc aagttgatgg ccccgttatt aaaagcctgg aaaggtgcgc
    21781 tgaaggcaga aaacgctgtc gatttttcgg gccttattca tcaggcgatt gtgattctgg
    21841 agaaaggtcg ctttatcagc ccgtggaagc atattctggt tgatgaattt caggatatct
    21901 cgccgcagcg ggcagcgttg ttagcggcat tacgcaagca aaacagtcag acgacgttgt
    21961 tcgctgttgg tgatgactgg caggcgattt accgattcag cggtgcgcaa atgtcgctca
    22021 ccaccgcttt ccatgaaaac tttggtgaag gcgaacgttg tgatttagac acgacttacc
    22081 gttttaacag tcgtatcggt aaggtggcaa accgttttat tcagcagaac ccaggccagc
    22141 tgaaaaagcc gctaaacagc ttaaccaatg gagacaaaaa agccgtcacg ttattggatg
    22201 agagtcaact tgacgctttg ctggataagc tctctggtta tgccaaaccg gaagagcgca
    22261 ttctgatcct ggcgcgttac catcacatga ggcctgccag cctggaaaaa gcggcaacac
    22321 gctggccgaa gttgcaaatc gactttatga ccattcatgc cagcaaaggg caacaggcgg
    22381 attacgtcat catcgttggc ttgcaggagg gaagtgatgg ttttccggct gcggcgcggg
    22441 agtcgattat ggaagaggcg ctactgccac cggttgagga tttcccggac gctgaagaac
    22501 ggcggttaat gtacgtggcg ctgacccggg cacgccatcg ggtatgggca ctgtttaaca
    22561 aagagaatcc ctctcccttt gttgaaatac tgaaaaatct ggatgtgccg gtggcgagaa
    22621 aaccgtaaga aacaggtggc gtttgccacc tgtgcaatat tacttcagac ggtccgcgag
    22681 ataacgctga taatcgggga tcagaatatc gaccgcgtcg ttgaaatgcg gcgactggat
    22741 tatgaagtct gccgttgcca cgttggcggc gaccggaatg ttccataccg tcgccagacg
    22801 cagcaaggct ttcacgtcag gatcgtgcgg cacggcattt agtggatccc agaagaaaat
    22861 caatacatca attttccctt ctgagatcaa tgcgccaacc tgctggtcac cccccattgg
    22921 gccactcaac atcgcgttga cgttcatgcc ggtcgcgcgg gaaattaagt taccggtagt
    22981 gcctgttgca tacagtacgt gttgttccag taacggttga tgccgttcca cccagctcat
    23041 cagcatttgt ttgcagtgat cgtgtgccac cagcgcaata tgtttccgcg caggtaaagt
    23101 gcgagtcgtc agttccataa tgtacatccg tagtcaactt tcctacagat tactgtaagc
    23161 acttatcgct gcaagataaa gaccgaaaaa gcctgcgcac aggcacaaaa atctcaggaa
    23221 gatggttgtt tttccgccca ctgcaggaaa gtatttcgcg tttgtgggtc agccagttta
    23281 aaccaatact tcagccgttg ttctgtgagc acctgagact gcggtggaat agcatccagc
    23341 tcggaaacac ccgaaagcag cgtactgtca tctgccatca tcgtggcaaa ttgcggtagt
    23401 gaagcgcgtt tcgcggattt gttatagcgt tcaacctcga cctcataatc aatcgttttt
    23461 gccgttgagg taatggcgag aatatccagc tttaccggaa tcggcgtcgc atcgccatcc
    23521 aacaattcaa ggcgcggcgc ggcatcaaaa tggttagctt cccgctcatt ctccaggcga
    23581 ggcaggcgaa aattgacctg gttgatgagc tgggtattaa aactgaccac tagcggtggg
    23641 gagatataca gccgttcttc actattggaa agatgaatcg tcttctcaac gcgaaacact
    23701 aactgatgcg gtccattatc cagttcaatg ctgtccgcac cgcgcagcag agagctggaa
    23761 accttcttgc cgtcgagtac cagaagatcg acatcggttg aaagccgcag cgtggtggca
    23821 aaaacggata ccggcaaaca taatgcaatc aaggtggtca cgatgccggt tttcataggt
    23881 agctcctgtc aaaagaccgc ccgaataata atgtgtcaaa atttttcttt aatcatactt
    23941 actaacatat agacatattt cccgcttttg ctctcattca ttcgtattag ctgcatggtt
    24001 ggcatgtcgg ctttgtcgta cactttgtta aacagtctgg aggaaataat gaaagaaacc
    24061 gatattgcag gcattttaac gtccactcac accattgcgc tggtgggggc aagcgacaaa
    24121 cccgatcgtc ccagctatcg cgtgatgaaa tatctactcg accaggggta tcatgtcatt
    24181 ccggtttcgc caaaagttgc cggcaaaacg ctgctgggcc agcaggggta tggcacgctg
    24241 gcagacgttc cggaaaaggt cgatatggtc gatgttttcc gtaattcaga agcggcgtgg
    24301 ggcgtggcac aggaagctat tgccattggc gcgaaaaccc tctggatgca actgggggtg
    24361 attaatgagc aagcggcagt actggcacgg gatgccgggt tgaatgtggt gatggatcgc
    24421 tgcccggcta ttgagatccc tcgcctgggc ctggccaaat aaaaaatccc cggaaggcaa
    24481 aaaccttccg gggattcgtt cagggatcag ttacgcagac gcggggcctg gagttgtttg
    24541 cggatggtct gcgccagctc atccatggat ggctgttcag gatgttcatc ctgcaattcg
    24601 ctacttagct gggcttcagc caggtaggta tgaaccggta ggccgttatc gtcctccatc
    24661 actacgtgat accacggagc ggcgcgaagc tcgtcattta ccgccagctc atcaggcgac
    24721 ggttcagaga gcgaataaac cgggtcgata tccacgacca ctccgagata acctaacagg
    24781 gaatggcgga cctgctggcc gataccgaat ttgctggcaa tcatagtcac ctcccgggaa
    24841 atctttacct ttgatataag ggtaaaattc cacatttcaa gttacatgac gcgacaggca
    24901 aaccctttca gatatagccc ttccggatag gtagcgatca ccggatgatc ggctgcctga
    24961 cggaactgct ctataaattg tacatcacgg ccggcatcaa ttgcggcatc cgcgatgatt
    25021 ttctgaaata aatcgctggt catcagaccg gaacaggaga aagtcaggag aataccgcct
    25081 tcattcagca actgaatcgc cagcatgttg atatctttat agccacgaca cgcgcccatc
    25141 aactggcttt tattctcaac aaacttcggc gggtccatca cgataacgtc aaatttttca
    25201 ccgcgatcgc gataagtacg cagcaattta aagacatcat cacggacaaa ctcagccttg
    25261 ctcagatcca gtttgttcag ctcaacgttc tgccgtgcaa tatccagcgc ttcctgggag
    25321 gtatcaacgc tgacaacctg gctgcaaccg cccatcagtg ccgatacggc gaaaccaccg
    25381 gtataggaga aacagttcag cacacgttta ttttcaacgt agcggcgggt agccaggcgg
    25441 ctgtctcgct ggtccaggta gtagcccgtt ttgtgtccgt gctgaatatc caccagcagc
    25501 ttcatgccgt gttcttcaat cggcagcagg gcaggcggca actcgccggt gacgaggccc
    25561 agggtcagct ccatcccttc ttttttacgt accgcaacat cgctgcgatc gtaaatcgca
    25621 cattccgggt acagcgtttg cagggcacta actaatgccg cgcgctgata ttctgcccca
    25681 gcactcagca gttgcagcac cagaaaatta ccgaaacgat cgatagtaat acccggcagg
    25741 ccatcagatt ctccggcgat taaacgatag ctgtcgaggc catctttttg cgccagccag
    25801 tcacgccatt tttgtgcttg ttgcaaacgg cgggaaaaaa aagcaatgtc gatagactca
    25861 gacgggtcaa acgtccagac gcgcgcccgg atttgcgaag ctggcgaata agcgccgcgt
    25921 gctaaccatt ttccctgatg atcaacaata tcgatggttt caccgaggct ggctttacct
    25981 tccatgcggg caacggcccc ggaaaagacc cacggatggc gacgaagtaa tgatttttcg
    26041 cgccctttgg ctaacactaa acgtacactc ataatttact tttctgtcga tgcaaagaaa
    26101 tgggcgtcat tgtccggagt tttccatgga tttgcaacgc gtccattaag gataaggaga
    26161 gcagcaatgt cgaaagtctg cataattgcc tgggtttacg ggcgagttca gggcgtagga
    26221 tttcgctaca ccacacagta cgaagcgaaa agactgggtt taaccgggta cgccaaaaat
    26281 cttgacgatg gcagcgtgga agtggttgcc tgcggtgaag aagggcaggt ggaaaaatta
    26341 atgcagtggt taaagagtgg cggcccgcgt tctgcgcgtg tagaacgggt gctcagcgag
    26401 ccgcatcatc cctcggggga attaaccgat tttcgtatcc gttaaataca ttttaccggt
    26461 tttggcaggc cagcaatttt ggtggcttgc ttagccggac ctttcggaaa cagtcggtat
    26521 aaatagcggc tgttaccttt ttcttcgcca aatttattcg ccatcgcttt taccagcata
    26581 cgaatcgccg gagaagtatt gaattccaga tagaaatcac gcacaaaacg caccacttcc
    26641 cagtgttccg gtgacagcga aatcccttcg ttctctgcaa tcaccactgc cagcggctca
    26701 ctccactggc tgctttcttt gagatagcct tcggtatccg tttctatctc tttaccttcg
    26761 aagatcagca taattattac taccttaatc agactggcgg cagtgtaaca aaaaacaaag
    26821 ccccgcataa agcgaggcta tgaaagtgtt agcgggtgag attaatcgcg gctggcgaag
    26881 cccagaatgc tcagcaggct gacgaagatg ttgtacagcg aaacatacag gctaaccgtg
    26941 gcacgaatat agttcgtttc accgccatga acgatgttgc tggtttcaaa caaaatagcg
    27001 ccagaggaga tcaaaatgaa gaccgcgctg atcgccagat gcagagcagg cagctgcagg
    27061 aagatattag caaccatacc aatcagcacc accacaatac ccgccatcag cataccgccg
    27121 aggaacgaca tatctttgcg ggtggtcagc acatatgcag agcagcagaa gaacactaac
    27181 gccgttccgc ccagagccat agcgattacg tcacccattc cggcagacag ataggtgttc
    27241 agaataggtc cgaggatata acccagaaaa ccggtaaagg cgaatgcgga gataatcccg
    27301 gtcggcttat tcgccgtttt ataggtcagg aacatcaaac catacatacc caccagcgtc
    27361 agaatcagac ccggagatgg cagcatcagc accgtactgg cagttgcggt aatcgccgaa
    27421 aaggccagcg tcaggctcag cagaaaatag gtattacgca gcaccttatg ggtgctaagc
    27481 agtgatgtac ggtcatgtga agaactaaca atacgatcca ttgagtcact ctctatgaca
    27541 gatgtaatta attaagcagc ataatgataa tgcgtaaggg cacccagaag ttttacccat
    27601 ctttacgcat ttgatctgga acaggtttaa cagcggatta tcaggtcatt aagcaaatat
    27661 aacgccctga gaatttcgac aggcaaaaga aaaaggggtt agcatttagc taacccctta
    27721 tcttatttgg cggaagcgca gagattcgaa ctctggaacc ctttcgggtc gccggttttc
    27781 aagaccggtg ccttcaaccg ctcggccaca cttccggaat gacgcgcact ataaacatcc
    27841 cgatgcggcg tgtaaacccc taatttgttt gtttgcctga aaaacagcca aaagtgcatt
    27901 gatagcgtga aataacagca gattgatcat ttcatcacca tgaattcctt ctcttttact
    27961 cgtttagcaa ccggctaaac atccccaccg cccggcaaaa agaaaaatag gtccattttt
    28021 atcgctaaaa gataaatcca cacagtttgt attgttttgt gcaaaagttt cactacgctt
    28081 tattaacaat actttctggc gacgtgcgcc agtgcagaag gatgagcttt cgttttcagc
    28141 atctcacgtg aagcgatggt ttgccttgct acagggacgt cgcttgccga ccataagcgc
    28201 ccggtgtcct gccggtgtcg caaggagaag agacgtgcga tatgaataac gaggaaacat
    28261 tttaccaggc catgcggcgt cagggcgtta cccggcgcag ctttctcaaa tattgtagtc
    28321 tggctgccac gtcgctggga ttaggcgcgg gaatggcacc aaagattgcc tgggcgctgg
    28381 agaacaaacc ccgcattccg gtggtatgga tccacggtct ggaatgcacc tgctgtaccg
    28441 aatcttttat ccgctccgct cacccactgg cgaaggacgt catcctttcc ctgatttccc
    28501 tcgattatga cgatactttg atggctgccg ccggaaccca ggcggaagaa gtcttcgaag
    28561 acatcatcac gcaatacaat ggcaaatata tcctcgcagt agaaggtaat ccgccgctgg
    28621 gcgagcaggg gatgttctgt atcagcagcg gtcgaccgtt tattgagaaa ctcaaacgtg
    28681 ccgctgccgg agccagcgcg attatcgcct ggggaacctg cgcgtcctgg ggctgcgtgc
    28741 aggccgcgcg gcccaatccg acgcaggcaa cgcctatcga caaagtcatc accgacaaac
    28801 ccattatcaa agtacctggc tgcccgccga tcccggatgt gatgagcgcc atcattactt
    28861 acatggtgac ctttgatcgc ttgccagatg tcgacagaat gggccgtccg ctgatgttct
    28921 atggtcagcg aatccacgat aaatgctatc gccgcgccca cttcgacgcc ggagagttcg
    28981 tccagagttg ggatgatgac gctgcccgca aaggttactg cctgtacaaa atgggctgca
    29041 aagggcctac cacctataac gcctgttcct ccacacgctg gaatgatggc gtttctttcc
    29101 caatccagtc tggtcacggc tgcctgggct gtgcggaaaa tggtttctgg gatcgcggtt
    29161 cgttctacag ccgcgtggtc gatattccgc aaatgggtac tcattccacc gccgataccg
    29221 tcggtttaac cgcgcttggc gtggtggcag cggctgttgg tgtgcacgca gtcgccagcg
    29281 ccgttgacca gcgcagacgt cataaccagc aacctacaga aaccgaacat cagccaggca
    29341 atgaggataa acaggcatga gcactcagta cgaaactcag ggatacacca tcaataatgc
    29401 cggacgccgc ctggtggtcg acccgattac gcgcatcgaa ggccacatgc gctgcgaagt
    29461 gaatattaac gatcagaatg tgatcaccaa tgccgtctcc tgcggcacca tgtttcgcgg
    29521 gctggagatc atcctacaag ggcgcgaccc gcgcgatgcg tgggcgttcg ttgaacgtat
    29581 ctgcggcgtc tgtactggcg tacacgccct ggcttcggtt tacgccatcg aagatgctat
    29641 cggtattaaa gtgccggaca acgccaatat catccgcaac attatgctgg caacgctctg
    29701 gtgccacgat catctggtgc acttctatca gcttgccggg atggactgga tcgatgtgtt
    29761 agatgcgctg aaagccgacc cgcggaaaac ctccgaactg gcgcaaagtc tctcctcttg
    29821 gccgaaatca tcccctggct atttcttcga cgtacaaaac cgcctgaaaa aatttgttga
    29881 aggcgggcag ttggggatct tccgcaatgg ctactggggg cacccgcagt acaaactgcc
    29941 gccagaagct aacctgatgg gctttgccca ctatctcgaa gctctcgatt tccagcgtga
    30001 aattgtcaaa atccacgcgg tctttggcgg taaaaacccg catccaaact ggattgtcgg
    30061 cgggatgcct tgcgccatca acattgacga aagcggcgcg gtcggggcag tcaatatgga
    30121 acgcctgaac ctggtgcagt caattatcac ccgcacggcg gacttcatta acaacgtgat
    30181 gatccccgac gccttagcca tcggtcagtt caacaaaccg tggagcgaaa tcggcactgg
    30241 tctttctgat aaatgcgttc tcagctacgg cgcattcccg gatattgcca acgactttgg
    30301 cgagaaaagt ctgctgatgc ctggcggcgc ggtgattaac ggcgacttca acaatgtgct
    30361 gccagtggat ttggttgatc cgcagcaggt gcaggagttt gtcgaccacg cctggtatcg
    30421 atatcccaac gatcaggtcg ggcgtcatcc gttcgatggc atcaccgacc cgtggtacaa
    30481 ccccggcgat gtcaaaggca gcgataccaa cattcagcag ctgaatgaac aggaacgcta
    30541 ctcgtggatc aaagcgccac gctggcgcgg taacgcgatg gaagtggggc cgctggcgcg
    30601 cacgttaatc gcttatcaca aaggcgatgc tgcgaccgtt gagtcggtcg atcgcatgat
    30661 gtcggcgttg aacctgccgc tttccggtat ccagtcaacg ttaggccgca ttttgtgccg
    30721 cgcgcacgaa gcgcagtggg ccgcaggtaa gttgcagtat ttcttcgaca agctgatgat
    30781 caacctgaaa aacggcaatc tcgccactgc ttccacggaa aaatgggaac ctgcaacctg
    30841 gccgacagag tgccgtggtg tcggttttac cgaagcgccg cgcggggcgt taggccactg
    30901 ggccgccatt cgcgatggca agattgatct ctaccagtgc gtggtgccga ccacctggaa
    30961 cgccagcccg cgcgatccca aagggcagat tggcgcttat gaagcggcgc tgatgaacac
    31021 caaaatggcg atccccgagc aaccgctgga gatcctgcgt actctgcaca gctttgaccc
    31081 gtgcctcgcc tgttcaacac acgtgctggg cgacgacggt agcgagctga tctccgtgca
    31141 ggtgcgttaa cagcgaagga gaatcatcat gcaacagaaa agcgacaacg ttgtcagcca
    31201 ctatgtcttt gaagcgccag tccgcatctg gcactggttg acggtgttat gcatggcggt
    31261 gttgatggtc accggatact ttatcggcaa gccgctacct tccgtcagcg gcgaggcgac
    31321 gtatctgttc tatatgggct acatcaggtt aattcacttc agcgccggga tggtttttac
    31381 cgtggttttg ctgatgcgga tctactgggc ttttgttggc aatcgatact cccgcgagct
    31441 gtttatcgtg ccggtatggc gtaaaagctg gtggcagggc gtgtggtatg aaatccgctg
    31501 gtatctgttt ctggcaaaac gtccgagtgc cgatataggc cataatccca tcgcccaggc
    31561 ggcgatgttc ggctatttcc tgatgtcggt ctttatgatc atcactggtt ttgcgctgta
    31621 cagcgaacac agccagtacg ctatttttgc gccgttccgt tatgtggtgg aatttttcta
    31681 ctggacgggt ggcaactcaa tggacattca cagctggcat cggctgggga tgtggctgat
    31741 tggcgcgttt gtgatcggtc atgtctacat ggcgctgcgt gaagacatca tgtccgacgg
    31801 cacggtgatc tccaccatgg tcaacggcta ccgtagccac aaatttggca aaataagtaa
    31861 caaggagtgt tcatgagcga gcaacgcgtg gtggtcatgg ggctgggcaa cctgctgtgg
    31921 gccgatgaag gcttcggcgt gcgggtggcg gaacggctgt atgcccatta ccactggccc
    31981 gagtatgtgg agattgtcga tggcggtact cagggactga acttgctggg gtatgtcgaa
    32041 agcgccagcc atctgttgat tctcgatgcc attgactacg ggctggaacc tggaacgctg
    32101 cgaacctatg ccggagaacg cattccggct tatctcagcg cgaagaaaat gagcctgcat
    32161 cagaacagtt tctccgaagt gctggcgctg gcagatatcc gcggacatct gccagcacat
    32221 attgccctcg tcgggctgca acccgcgatg ctcgacgact acggcggcag cctgagcgaa
    32281 ctggcacggg agcaactgct cgctgcggaa caatcggcgc tggcgcagtt ggctgcgtgg
    32341 ggaagtgtgc cgcaactggc taatgaatcg cgttgtctca attatgactg tctgtcgatg
    32401 gaaaattacg aaggcgttcg cttgcgccag taccggatga cacaggagga gcagggatga
    32461 gcaacgacac gccatttgat gcgttgtggc aacgaatgct ggcgcgcggc tggacgccgg
    32521 tcagtgaatc ccgtcttgac gactggctta cgcaagcgcc agacggcgtg gtgttattaa
    32581 gcagtgaccc gaaacgcacg ccagaggtca gcgataatcc ggtaatgatt ggcgaattac
    32641 tgcacgagtt tcccgactat acatggcagg tggcgattgc tgaccttgag cagagcgaag
    32701 ccatcggcga tcgctttggc gtctttcgct ttcctaccac tttagtgttt accggcggaa
    32761 actatcgcgg cgtgctgaat ggtattcacc cgtgggcgga actgataaac ctgatgcgcg
    32821 ggcttgtcga accgcagcag gagcgtgcct catgagcgaa acttttttcc atctgctggg
    32881 gccaggaacg caaccgaacg atgacagttt cagcatgaat ccactgccga tcacctgtca
    32941 ggtgaatgat gaaccgagta tggcggccct ggagcaatgt gctcacagcc cgcaggtgat
    33001 tgcgctgtta aacgagttac aacatcaact aagcgaacgc cagccgccgt tgggcgaggt
    33061 gctggcagtc gatctgttaa atctcaacgc cgacgatcgt cactttatca atacgcttct
    33121 cggggaaggg gaagtgtcag tgcgcattca gcaggctgac gacagtaaaa gtgaaataca
    33181 ggaggcgatc ttctgcggat tatggcgggt gcgcagacgt cgcggcgaaa agttgctgga
    33241 ggacaaactg gaggctggct gcgcgccgct ggcgttgtgg caggcggcaa cgcaaaatct
    33301 cttgccgaca gattcgctgt taccgccgcc cattgatggc ctgatgaatg gcctaccgtt
    33361 ggcgcatgag ttactggcac atgtacgtaa ccccgacgcg cagccgcaca gcattaatct
    33421 gacgcaatta cccatcagcg aggctgatcg gctttttctc tcacgtctct gtgggccggg
    33481 aaatattcag attcgtacca ttggctatgg cgagagctat atcaacgcca cggggttacg
    33541 ccatgtctgg catttacgct gtacggacac cttaaaaggc ccgttactgg aaagttatga
    33601 aatctgccca ataccggaag tggtgctggc agcgccagaa gatttggtcg actctgcgca
    33661 gcggcttagc gaggtatgtc agtggctggc ggaagctgca ccgacgtaaa aagacggtaa
    33721 gtatcgcttt cagtcttatg aatatcgcaa tcggcgaata cctctggtcg tagagtttca
    33781 ggataaagag ggagatctaa cattatcggg ttatttttct ctcttcgcct acaggagtgc
    33841 gcatgtggga tgtcattgat ttatcgcgct ggcagtttgc tctgaccgcg ctgtatcact
    33901 ttttatttgt accccttacc ctggggctga tttttttgct ggctatcatg gaaaccattt
    33961 acgtggtcac cggcaaaaca atctaccgcg atatgacgcg cttctggggt aagctcttcg
    34021 gtatcaattt tgctcttggc gtggctaccg gcctgaccat ggagtttcag tttggtacta
    34081 actgggcatt ctattccaac tatgtgggcg atatttttgg cgcaccgctg gcgatggaag
    34141 cattaatggc cttcttcctc gaatccacct ttgtcgggct tttcttcttc ggctggcagc
    34201 ggctgaataa ataccagcac ctgctggtga cgtggctggt ggcgttcggt tcaaatctct
    34261 ctgcattgtg gatattgaat gccaacggtt ggatgcaata cccgaccggt gcgcattttg
    34321 atatcgacac cctacgtatg gagatgacca gcttcagcga gctggtcttt aacccggtca
    34381 gccaggtgaa atttgtgcac accgtaatgg cgggctacgt gaccggggcc atgtttatta
    34441 tggcgatcag cgcctggtat ttactgcgcg gacgggagcg cgatgttgca ttacgctcgt
    34501 ttgccatcgg ttccgtcttc ggtactctgg cgattatcgg cacgctacaa ctcggggaca
    34561 gttctgcgta tgaagtcgcg caagtacaac cggtaaaact ggcggcgatg gaaggggagt
    34621 ggcaaacgga acctgctcct gcaccgttcc atgtggttgc ctggccggaa caggatcaag
    34681 agcgtaacgc ctttgccatc aaaattcccg cgctgttagg gatcctcgcc actcactcat
    34741 tagataaacc cgtgccgggt ctgaagaatt tgatggctga aacctaccca cgcttgcaac
    34801 gcggacgtat ggcctggctg ttaatgcagg aaatatcgca aggcaatcgt gagccgcatg
    34861 tgttgcaggc attccgggaa ctggaaggtg acctgggcta cggcatgttg ctctcccgct
    34921 atgcgccgga tatgaatcat gtcacagccg cacagtacca ggcggcgatg cgtggcgcga
    34981 tacctcaggt tgcgccggta ttctggagtt tccgcatcat ggtgggctgt ggttccctgc
    35041 tgctactggt gatgctgatt gcgcttgtcc agacgctgcg tggcaaaatc gaccagcatc
    35101 gctgggtgct gaaaatggcg ctctggagtt tgccgttgcc gtggattgcg attgaagccg
    35161 ggtggtttat gaccgagttt ggtcgtcagc cgtgggcgat acaggacatc ttaccgacat
    35221 actccgcgca ctccgcttta accacaggac aactggcttt ctcactgatc atgatcgtag
    35281 ggctttacac cctgttctta atcgccgaag tctacctgat gcagaaatat gcccgtctgg
    35341 ggccgagcgc gatgcagagt gaacaaccga cgcagcaaca ggggtaaagg agaaaatcat
    35401 gtttgattat gaaacattgc gcttcatctg gtggctgctg attggcgtga tcctggtggt
    35461 ctttatgatc tccgacggat ttgacatggg gatcggctgt ctgctgccgc tggtggcgcg
    35521 taatgatgat gaacgccgga tagtgataaa cagcgttggt gcacactggg aaggcaacca
    35581 ggtctggttg atcctcgctg gtggggcatt atttgccgcc tggcccagag tgtatgcagc
    35641 ggcgttttcc ggcttttatg tggcgatgat cctggtgctg tgctcactgt tcttccgccc
    35701 gctggccttt gattatcgcg gaaaaatcgc cgatgcacgc tggcgtaaaa tgtgggacgc
    35761 cggtctggtc atcggcagtc tggtgccgcc ggtagtcttc ggtatcgcct tcggcaactt
    35821 gttgctcggc gtgccgtttg ccttcacacc gcaattacgc gtggagtatc tcggcagctt
    35881 ctggcaactg ctgacgccat tccctttatt gtgcggattg ctcagccttg ggatggtaat
    35941 tttgcaaggt ggcgtctggt tacaactgaa aactgttggt gtgattcatc tgagttcaca
    36001 gctggccacc aaacgcgctg cactgttggt gatgctgtgc tttttgctgg cgggttactg
    36061 gctgtgggtc ggtattgatg gctttgtact gctcgcccag gatgctaacg gtccttccaa
    36121 tccgttaatg aaactggtgg cagtgctacc tggtgcctgg atgaataatt tcgtcgaatc
    36181 gcccgttttg tggatcttcc cgctgctggg gttcttctgc ccattgctga cggtgatggc
    36241 gatttatcgt ggtcgcccag gttggggatt tttaatggcg tcattgatgc aatttggcgt
    36301 gattttcacg gcaggcatca cgctgttccc cttcgtcatg ccgtcaagcg tgagtccgat
    36361 ctccagcctg acgttgtggg acagcacttc cagccagctg acgctgagca ttatgttggt
    36421 aatcgtgctg atatttttgc ccattgtgtt gctctacact ctctggagct actacaaaat
    36481 gtgggggcgc atgacaacag aaactctccg ccgtaacgaa aacgagttgt actaaggagc
    36541 aggaacaatg tggtatttac tttggttcgt cggcattttg ttgatgtgtt cgctctccac
    36601 ccttgtgttg gtatggctgg acccgcgtct gaaaagttaa cgaacgtagg cctgatgcgg
    36661 cgcattagca tcgcatcagg caatcaataa tgtcagatat gaaaagcgga aacatatcga
    36721 tgaaagcgat cttaatccca tttttatctc ttctgattcc gttaacctcg caatctgcat
    36781 tcgctcagag tgagccggag ctgaagctgg aaagtgtggt gattgtcagt cgtcatggtg
    36841 tgcgtgctcc aaccaaggcc acgcaactga tgcaggatgt caccccagac gcatggccaa
    36901 cctggccggt aaaactgggt tggctgacac cgcgcggtgg tgagctaatc gcctatttcg
    36961 gacattacca acgccagcgt ctggtggccg acggattgct ggcaaaaaag ggctgtccgc
    37021 agcctggtca ggtcgcgatt attgctgatg tcgacgagcg tacccgtaaa acaggcgaag
    37081 ccttcgccgc cgggctggca cctgactgtg caataaccgt acatacccag gcagatacgt
    37141 ccagtcccga tccgttattt aatcctctaa aaactggcgt ttgccaactg gataatgtga
    37201 acgtgactga cgcgatcctc agcagggcag gagggtcaat tgctgacttt accgggcatc
    37261 ggcaaacggc gtttcgcgaa ctggaacggg tgcttaattt tccgcaatca aacttgtgcc
    37321 ttaaacgtga gaaacaggac gaaagctgtt cattaacgca ggcattacca tcggaactca
    37381 aggtgagcgc cgacaatgtc tcattaaccg gtgcggtaag cctcgcatca atgctgacgg
    37441 agatatttct cctgcaacaa gcacagggaa tgccagagcc ggggtgggga aggatcaccg
    37501 attcacacca gtggaacacc ttgctaagtt tgcataacgc gcaattttat ttgctacaac
    37561 gcacgccaga ggttgcccgc agccgcgcca ccccgttatt ggatttgatc atggcagcgt
    37621 tgacgcccca tccaccgcaa aaacaggcgt atggtgtgac attacccact tcagtactgt
    37681 ttattgccgg acacgatact aatctggcaa atctcggcgg cgcactggag ctcaactgga
    37741 cgcttcccgg tcagccggat aacacgccgc caggtggtga actggtgttt gaacgctggc
    37801 gtcggctaag cgataacagc cagtggattc aggtttcgct ggtcttccag actttacagc
    37861 agatgcgtga taaaacgccg ctgtcattaa atacgccgcc cggagaggtg aaactgaccc
    37921 tggcaggatg tgaagagcga aatgcgcagg gcatgtgttc gttggcaggt tttacgcaaa
    37981 tcgtgaatga agcacgcata ccggcgtgca gtttgtaatg cataaaaaag agcattcagt
    38041 tacctgaatg ctctgaggct gatgacaaac gaagaactgt ctaatgcgta gaccggaaaa
    38101 ggcgttcacg ccgcatccgg ccactttcag ttttactctt tctcggagta actataaccg
    38161 taatagttat agccgtaact gtaagcggtg ctggcgcgtt taatcacacc attgaggata
    38221 gcgcctttaa tattgacgcc tgcctgttcc agacgctgca ttgacaaact cacctctttg
    38281 gcggtgttca agccaaaacg cgcaaccagc aggctggtgc caacagaacg ccccacgacc
    38341 gcggcatcac tcaccgccag catcggcggc gtatcgacaa tcaccagatc gtaatggtcg
    38401 ttcgcccatt ccagtaattg acgcatccga tcgcgcatca gcagttcaga cgggttaggt
    38461 ggcacctgac cgcgagtaat cacatcaaag cctcctttgc caaaatgctg gatcactttg
    38521 ttgagctcat ctttacctgc cagatattcc gacaagccat gttcattact cacggtaaac
    38581 aggttatgcg aataaccacg gcgtaagtcg gcatcaataa ataacacttt ttgatcggac
    38641 tgggcgatca ccgctgccag agttgaactg acaaacgttt taccactgtc tggcgtcgca
    38701 ccggtgatca tcagaatgtt attctccgtc tccatcatag cgaagtgcag actggttcgt
    38761 agcgcacgta cggcttccac agcagaatcc gccgggttat ccaccgccag gaaggggata
    38821 tttttagtac gatggcgctg ctgattagaa aataaatttt tcttacgcag acgggtacgt
    38881 ttatccagcc actcggacat tgggatagtg gcataaacgc tgatgccgtg ctcttccagt
    38941 tgttccgggg cttctacacc acgacgcaac atcgcacgcg ccagcacggc acccaccgaa
    39001 ataaacaggc caagaataaa accaagcacc acgttcaacg cttttttcgg tttcactggc
    39061 tgcggctgag tgactgctgg gtcgataatc cgcacgttac caatggcact ggatttcgaa
    39121 atactcaact cctgctggcg gttaagtaat tgcagatata ccgcacggcc cgcttctacg
    39181 tcacggctta aacgtaacac ttcctgttga gtggaaggca ttgccgatac ccgcttattc
    39241 aggcgtttgc gttcttgctc cagcgtctgg cgtttttcca gcagcgcacg ataagttggg
    39301 tgatctttct tatacagctg ggagatctct gcctcgcgga aagtcagctc attgagttga
    39361 ttatcaacgt tcacaatctg ctcaagaacg gctttggctt ctaggttaag gtcaaccgaa
    39421 tcgcgctgct ggcgataaac gttgagtttt tcttccgcct ggtccagctc gctgcgcact
    39481 tcaggcaact ggcgctgtaa gaattcaagg ctttgtgaat cctgcgccgc ctggcgagcg
    39541 atattctgtt gcaaatagtt gttagcgatg ctgttcagaa tacgagtaat caactgggga
    39601 tcatcaccag tcatggtaag ttccagcatc ccgctttctt tactgcgttc gctaacggta
    39661 aaggtttcct gcaatgcgtt aatcgcttcc agttcggtac gctggctcag gacaaactgt
    39721 gttcctggtt tggccttaat gtccgcgata gtcagcgcaa cgccatcttt ttccagacgc
    39781 tggccgacca taccattgac ggtgaactct tcaccttcca gtgtatagtg gccgttttcc
    39841 ccaaccgtga gtgtcagttg ctgatcctga ccattcagtt gtggaatatg catccagctg
    39901 atcgccagct cacctggttt ttctttggtt aatctcgccc agccgcgacc cacaatcgga
    39961 aaatacttct gctcaactat gtcgtgcaga ttcagttcag caatggtttt accgagaatc
    40021 atgcgcgatt gcagcagttg gatctccggt gcagactcgg gcgatgagtt agggatcata
    40081 tcgctcaggc cgctgagaat ggcgttgccc tgtttttgct caacctggac cagagtatct
    40141 gcctgataaa ttggtgtgct taacagcgag taagcgacag cgatcagcgt gaataacgcg
    40201 gttacgctga taataaactt acggtgatcc cataactcgc cgaccagacg aagcagatcg
    40261 atctcatttt cctgagtgct gcctggtggc gtattcatat ttttagttgt catcgtattt
    40321 tataaactca aattaccggc tgaggcgctt cgcccattcc tgactggcgc gctccaacat
    40381 accgtagaca tgttcaaatg cgtcctgact tttacgatag ggatccggga tctctttctg
    40441 ttccagccat tgcccaaaca gcattgtctt cccgcggacc tcgggagcga ttgcggtaac
    40501 ctgagcaata tgctctgact ccattgccag aatcagatcg taatttcgtg ccatctcagc
    40561 agtgagctta cgtccggcat gtccttcaag agatacgcca tgattagcgg caacatctgc
    40621 tgctgtcgca tctgcagggt gttttaccag accatgaaca cctgccgatt tgacttttac
    40681 gcctggcaga cgtttacgca gcaagcgctc gccaatgggc gaacggcaaa tgttgccggt
    40741 acaaaccacc aggattgagt taaattttag ttgggccatg tttgaatcca ccgtgaagtc
    40801 tctgtcaggt catgcacccc ggaaatcgtc ggtaccagca gagaaatcac acggttccaa
    40861 cgagccagcg gcgcggtagt gacataaaca atgtcgtaag gttcaagctg gaattccgtt
    40921 ccgagtatca ttgccgatgc gtccttcgca ttcagctgat aaatattggc gattttgcca
    40981 ttctgcttgt tttgcgttgc acgaataaca aaaatccccg tcgcatcagc gacatcctga
    41041 ttcataccct cagcgttacc cagcgcttcc gccagggtca taccgctgcg gtccattttc
    41101 agcgtgctct gtttacccac ttcacccatc acgaagactt tcagatcatc gttgcgcgga
    41161 ataaataaaa tatcgcccgg gtgcagcagt ttgttctggc gcaagtcgcc acgctgcatc
    41221 agcgcataga ggttcacttt ggtctttacg ccattctggg tgagcaccac gttgcgccag
    41281 tctgcatctg cggtcagacc gcccgcggcg ttaatcgcat ccatgatggt cagaggaata
    41341 ttggtaatcg gctgctgacc cgatttgctg acttcgccgg tgacgtacgc tttttgcgat
    41401 cggaacgccg cgacgctgac atcgacttgc gggctttcaa tgactgaatc gagacgcgct
    41461 gtaatctcgt tgcgtacctg ggtaagtgtt ttcccggcca ctttcaggcg accaatatag
    41521 gggtagaaaa tcgcaccatc ggcgttaacc cagttgccgg tatcactggc gctacggtat
    41581 tgccctgcgg gggtggttag ttccgggtga tcccagaccg taaccatcaa cacatcacca
    41641 atcccgatac gatattcata gtttgcgatt tgctgctcca gctccgggtt agcctgcgac
    41701 ataatcggtt tggcgcgcaa ctgatcaatc aatcctggcg tgacgggata gatattgacc
    41761 attttgtcca ggtcatactg attgtcaggt agttcaatga catccttgtt cctggtcgat
    41821 aaattctgac caggaacaag ggtacatgct gtcaaagaca gaacagccaa tgtcagtacc
    41881 gaaaacttaa aaatgttctt tttcattggt ttatatttat cagtcaatgt aatgttatga
    41941 attacgtcag gttattgtcc aaccggtact tctcgctctg ccgtcattgg atagagctgg
    42001 tatttgcgtg acagcatttg tccaccatca cgcgtcaatg gtgtccacga aaccaccgcg
    42061 cggttgcggt taggtccaat ggtcatcaag tcgaatggaa tcgagatata aaaaccttta
    42121 ctaaagccgc cttcgccgta gtcatcttta gatacgttac tgattgccgc ccatacccct
    42181 accgccacgc cgctgtcaaa gcgtttggcg acgtcgatcg ttgctccttt atcttttgcc
    42241 agatattgcc caacgctaag tttcatcagt acgccattga gcgtcggcgg gttccagtaa
    42301 gccgtcacga aaccagttgg cgtggaataa tcggtgaagc gcatcatgtt gtcccagtca
    42361 cgttgcttca cgtagttaac gtccacaccc agcgcccagc tggcatctag cgggcgataa
    42421 agcagctcgg aaccgacacc ggcgtacatc gtttccagat aaccgccata cacctggcca
    42481 tagaaaccat tgcctaagtc ggcaaagtag ttcgcctgca agttgttgag ataaacgtca
    42541 ttgcgaacgt aatcacggat atgcgtgcgc acgcggggca gggtagagtc cgcgggcaac
    42601 agcgaagact taaacttgtc gtagttgttg taaatattgg tgaaaatacc gccatcaagc
    42661 agcaggtggt cggtaaacca gtagcgggca ctggacatca gccccagctg gaacatatag
    42721 aaatcttccg ggccgcccag cgactgtgaa agtgttgggt tgaaagagta gctaaagcga
    42781 tcttcacgaa tacggtagcc ccgaccaaag gcagaaagat cttctgcttc aacacgttgt
    42841 tgttgaagtt gctctgattg accaggcgct gtaccagcca gctgtttgcg caggctggct
    42901 acgtcggttt cggtagtcac catcgccata tgctcgcgct tttgcgtcac gctaatcttc
    42961 tcaacgcctt gcggcaggtt attcaccaga atccggttgg cacgatcgac cgcttcgcga
    43021 gaatcacggt atttgtattg ttgaccagac atatacagcg tcttatcgcg cagctgaatt
    43081 tccggtgctt caaaacctgc gttgtacttc agcgcggtaa gttggttcgc tactgtggtg
    43141 tactgcaatc cttcagattc aggcgcaggt tgatatgccg gttttggcgt atcgcgcagg
    43201 gcagggcgca gatcgttgaa attggtccgt aacgtgaagc caaacatcaa cgtgttaccg
    43261 cgttcataac tcaggttgag atctgcccag ctggcagcgc gataaactgc gccgacgttg
    43321 aaatggcttg cctgaggcag tttgccagcg aaatcattct ggtaattgtt gccatcgtat
    43381 tcgagtttca gacgcagggg attccacggc gtttgatact caatgccgcc aaagatggaa
    43441 gccgggccac gaaagatatc gctaaagctg atatcgcccg catcgtgaga ctctgcgcga
    43501 tgacaatatt tatcgttcac ccggcaaaac gggttggtaa tattgcccgc attaccggcg
    43561 tagccccatg ccatcccgag ggtgaaatca aatggccccg cttgcttgct ggccaccaga
    43621 tactcaccgt caaacagacc cgtaccagca atatcacgtt taccaaacgc cacttgcggt
    43681 agccagtaac cttcttccca caggcgaagt ttaaaatcga atgatttgtc tttgtatgac
    43741 tgatcgccgc tgaaatcttc ccactggcta tatttgcgtg tgcgcacatc cgtataacga
    43801 atggtgcctt ccagccatgg gaacagcgcc acggaggtgg agtagaaccg gtattgatcg
    43861 ttatcccggt agttgacgct gaattcacct tccggtgcga tgcgcgcatt tggcatctgc
    43921 aacaatcctg tgccgccaaa gtctgattgc gacgaaccca gcggatccgg gtaggttaat
    43981 acttcagcat gacaggcact ggagacggca atggccaggc agcttaaaag ataagaattc
    44041 ttcttcatca gtcaggaaca cgttgcgtta aaacagacac aatctggtca tttaaatcag
    44101 catatttttc gggcagtaca tgcgcagaaa agcccaacca aagctggctg ccaggcggtg
    44161 gttccacatg tcttttattc cacagggcaa caggggcgac gacagtctca ccctcaggcg
    44221 taatcaccat aacgttattc ttgtccgcgc ctgcaaggcg ggggtgatct tgcagataat
    44281 cggtaacgct acggcctgcc tgccagggca attgcccggc cccagaaacc gcacccagca
    44341 gagtaatagt caccggacgc tgtacggtat agagcgtata gtccccgacc aacgggggat
    44401 tgctgttctc atccactcga acaaaatcag ggtccagttt gaccggtaag cgaccagtga
    44461 tattgagatt gagtaactgc tggcgcacag atttaatcgt tgccgccaca tcgtcgtcag
    44521 cttctgcttc ccaggaagca agctgtgcca tgacatgttg gtagtcttta agcgctttag
    44581 ctttcgccgc agagtcggtc agcaaggcac caggccacca gaggcgatcg cgcagttgcg
    44641 gttgtgtcac caactggaca acattttcca tggggccaac ggaaagtgtc tgttgttcgc
    44701 caggcagata aatagtcacc gttccttgcg caaaggcatg gggtgtcatt acgtaaagta
    44761 cgctggcaat gaaatacgac tgtaatttat tcatggtgcg ggtttaagaa acgtcatttc
    44821 tacgggaata acccctgcgc ctaacatttg ccgactttga cgaacctggc cagtagccga
    44881 atcgacccag aatgtgtttt gccactgttt ttctggccgc gtggaagtca cctcttcctg
    44941 ccagacatta cacagcacat tgcgacccgc aatattcagc gtctctaatc cagcaaagga
    45001 aaaagtagaa ctcagggtcg cagagcgaaa atggttgtct tcactccaga gaatatcgcg
    45061 cgtccagcgg ctgccatctt ttattgccag cgcgttacgc agagggtcct gcccggaatt
    45121 tgtcacttcc agcagattat tattaagttt gacggttttt aaaagacgtc cattgtgggt
    45181 aaccagcatg gcgttatcct gggataacca tttgctttgt tcttgttcta tatatcccag
    45241 taccacaaag attcgttgcc cttcattaag gcgtaaatac atcgtggaat aaggcaacgc
    45301 ctggatctgc tgatccgcta cggtgatatc ctgattatcg aaaaggctgg cgcgaaatgt
    45361 atcgaccata ctttgctggc tgtgcgtaca tcccgcaaga aatagtgcga aaatcgataa
    45421 aataagaggg cgcaccttta atccttgaaa ggttaattaa aaaaatgcaa atctttaaag
    45481 cccggtacga atatcgcaac cgggtatcat ttaactttaa tttcgacgcg ttaaagcagt
    45541 attagcgtgt agtggacgtg gtggttgagg tgttatgaga accgccatcg ccactgtcat
    45601 ttgctgcaac aactgccaac gtcgccacac caacagccgt tgccacaccg acaactgtcg
    45661 ccgtgccgac agcgccagca gccacgccac cagtggtagt agcagtcgtg gttgcgccgg
    45721 tagtggtcgt tgtggtgcca gtagtacccg tcgttgcctc ggtcccgttt gttgcttcag
    45781 gggcagcaaa cgcagctggc gtcgtcaaca tgaaggccat aaggattgca gaaagcttgt
    45841 gtttcataac ttttccttta ttcatcgcat ggacaatacg tacgcttgag atcaggattg
    45901 tctactttga gtataacatc cttgtgaaat ctccctttcc tcataccgct tgcactatat
    45961 actgcgggta ggaaaggcgc gcagagggaa atataagatt gttaactatc cccctctgga
    46021 aatacgactt gtgagcataa gataaatctt agcaaaatac aaattaaaga taaggaaatt
    46081 gttacgaaag ctattaatta ttgctggtaa tatagtttca agtggtacta taactggctg
    46141 ttaaatggca tttataacta ttaggtgcag agatattcgc ttaaatggat tagtttcttt
    46201 gtggaaaagt aactgatgtt ataatatatg atgttgacta ttgcactatt ccaataaaac
    46261 cagtcagctt taaacaagca gcgtcatatt aagagagata aacatttgcc gctgttggtc
    46321 ctcgcaggcc atttacgcgg caaaattcca cacgtaatcc tggtataagc acttctgcgt
    46381 cgcggggagt gaatgcggaa atatggacct gaacttcttt acgaccgtcg gaggggataa
    46441 tgaatccttt gccgcttttg cgatcaaagg ttttgacaat tcctgtcatt ttacgggaca
    46501 aacaaattcc ttactgaaaa tactgcgctg cactatacgg ggttaataaa ataaagccag
    46561 cgatatttaa gaccgccgga cggctaaaat aaaatttgct taatctcaat tatcatgcgt
    46621 taatagctgc gtcggtttga aagacagaca gcatacaaag tagtttacta aagcagttct
    46681 cattatcagg cattatcccc ttcttttgag tctctctcct gaacactaag tagtttctgt
    46741 attaaagccc tgtttgccga aaggcccaaa atgaaggaag taaaatatgt ctaataaaat
    46801 gactggttta gtaaaatggt ttaacgcaga taaaggtttt ggctttatca ctcctgatga
    46861 tggcagcaaa gacgttttcg tccatttcac tgccatccag agcaatgaat tccgcacgct
    46921 gaacgaaaat cagaaagttg aattttctat tgagcagggg caacgtggcc ccgcggcagc
    46981 gaacgttgtt acgctctaag gttgccgtta ttactcaaca tctccatttc cgctgtccat
    47041 gttgtcatgg ttcacagtac cgcacatcgg cattcgatgt gacggagcga aatcctttgg
    47101 gcgctaagtg tattttttgt aaatcgacga tgatcacctt tgataacgtc acgctgcaaa
    47161 tacgcactga ccatgcgccg ctggatttca caaaataata tcaggctccc tcgtggagt
//
LOCUS       DAJTSL010000008        43881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTSL010000008 DAJTSL010000000
VERSION     DAJTSL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.008"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            85..609
                     /gene="lpfE"
                     /locus_tag="OO433_000430"
     CDS             85..609
                     /gene="lpfE"
                     /locus_tag="OO433_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HCR6049033.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            862..2553
                     /gene="eptB"
                     /locus_tag="OO433_000431"
     CDS             862..2553
                     /gene="eptB"
                     /locus_tag="OO433_000431"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6049034.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            2645..2721
                     /locus_tag="OO433_000432"
     tRNA            2645..2721
                     /locus_tag="OO433_000432"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2679..2681,aa:Pro,seq:cgg)
     gene            3465..5072
                     /gene="dppA"
                     /locus_tag="OO433_000433"
                     /pseudo
     CDS             3465..5072
                     /gene="dppA"
                     /locus_tag="OO433_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
     gene            5380..6399
                     /gene="dppB"
                     /locus_tag="OO433_000434"
     CDS             5380..6399
                     /gene="dppB"
                     /locus_tag="OO433_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6049035.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPASGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            6409..7311
                     /gene="dppC"
                     /locus_tag="OO433_000435"
     CDS             6409..7311
                     /gene="dppC"
                     /locus_tag="OO433_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6049036.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDMLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            7322..8305
                     /gene="dppD"
                     /locus_tag="OO433_000436"
     CDS             7322..8305
                     /gene="dppD"
                     /locus_tag="OO433_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6049037.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            8302..9306
                     /gene="dppF"
                     /locus_tag="OO433_000437"
     CDS             8302..9306
                     /gene="dppF"
                     /locus_tag="OO433_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6049038.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(9336..10607)
                     /locus_tag="OO433_000438"
     CDS             complement(9336..10607)
                     /locus_tag="OO433_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6049039.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            11083..11190
                     /locus_tag="OO433_000439"
     CDS             11083..11190
                     /locus_tag="OO433_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6049040.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(11277..12956)
                     /gene="bcsG"
                     /locus_tag="OO433_000440"
     CDS             complement(11277..12956)
                     /gene="bcsG"
                     /locus_tag="OO433_000440"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6049041.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVETTGGAPVVGDMPAQTTPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTTVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(12953..13144)
                     /gene="bcsF"
                     /locus_tag="OO433_000441"
     CDS             complement(12953..13144)
                     /gene="bcsF"
                     /locus_tag="OO433_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6049042.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(13141..14712)
                     /gene="bcsE"
                     /locus_tag="OO433_000442"
     CDS             complement(13141..14712)
                     /gene="bcsE"
                     /locus_tag="OO433_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6049043.1"
                     /translation="MRDIVGPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLTHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            14985..15173
                     /locus_tag="OO433_000443"
     CDS             14985..15173
                     /locus_tag="OO433_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6049044.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            15185..15937
                     /gene="bcsQ"
                     /locus_tag="OO433_000444"
     CDS             15185..15937
                     /gene="bcsQ"
                     /locus_tag="OO433_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6049045.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHRQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            15934..18552
                     /gene="bcsA"
                     /locus_tag="OO433_000445"
     CDS             15934..18552
                     /gene="bcsA"
                     /locus_tag="OO433_000445"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR6049046.1"
                     /translation="MSILTRWLLIPPVNARLIGGYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFILRRP
                     ERSETAQPSDQALAQQ"
     gene            18563..20901
                     /gene="bcsB"
                     /locus_tag="OO433_000446"
                     /pseudo
     CDS             18563..20901
                     /gene="bcsB"
                     /locus_tag="OO433_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            20908..22014
                     /gene="bcsZ"
                     /locus_tag="OO433_000447"
     CDS             20908..22014
                     /gene="bcsZ"
                     /locus_tag="OO433_000447"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6049047.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            21996..25469
                     /gene="bcsC"
                     /locus_tag="OO433_000448"
                     /pseudo
     CDS             21996..25469
                     /gene="bcsC"
                     /locus_tag="OO433_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            25551..27539
                     /gene="hmsP"
                     /locus_tag="OO433_000449"
     CDS             25551..27539
                     /gene="hmsP"
                     /locus_tag="OO433_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6049048.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            27722..29008
                     /gene="dctA"
                     /locus_tag="OO433_000450"
     CDS             27722..29008
                     /gene="dctA"
                     /locus_tag="OO433_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6049049.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPVSVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            29228..30724
                     /locus_tag="OO433_000451"
     CDS             29228..30724
                     /locus_tag="OO433_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6049050.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(30820..31749)
                     /gene="kdgK"
                     /locus_tag="OO433_000452"
     CDS             complement(30820..31749)
                     /gene="kdgK"
                     /locus_tag="OO433_000452"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6049051.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDISLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSTAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            31981..32748
                     /gene="pdeH"
                     /locus_tag="OO433_000453"
     CDS             31981..32748
                     /gene="pdeH"
                     /locus_tag="OO433_000453"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6049052.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLGDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            32818..34878
                     /locus_tag="OO433_000454"
     CDS             32818..34878
                     /locus_tag="OO433_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6049053.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEQLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(35112..36434)
                     /locus_tag="OO433_000455"
     CDS             complement(35112..36434)
                     /locus_tag="OO433_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6049054.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLLILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETQHQSL"
     gene            complement(36845..37858)
                     /gene="yhjD"
                     /locus_tag="OO433_000456"
     CDS             complement(36845..37858)
                     /gene="yhjD"
                     /locus_tag="OO433_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6049055.1"
                     /translation="MTQENEIKRPTQDLELEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMLGK
                     TQP"
     gene            complement(37907..38806)
                     /locus_tag="OO433_000457"
     CDS             complement(37907..38806)
                     /locus_tag="OO433_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6049056.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKNLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            39326..39928
                     /locus_tag="OO433_000458"
     CDS             39326..39928
                     /locus_tag="OO433_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6049057.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(39979..41628)
                     /locus_tag="OO433_000459"
     CDS             complement(39979..41628)
                     /locus_tag="OO433_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6049058.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVKRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            42032..43429
                     /gene="ccp"
                     /locus_tag="OO433_000460"
     CDS             42032..43429
                     /gene="ccp"
                     /locus_tag="OO433_000460"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6049059.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            43640..>43881
                     /locus_tag="OO433_000461"
     CDS             43640..>43881
                     /locus_tag="OO433_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6049060.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT        10540 a        11002 c        11925 g        10414 t
ORIGIN      
        1 tcccgttagc actaccggaa aattacccgc tgccggggat tatagcggta ttgctaccat
       61 acatgtcgaa ctggagtaac agtcatgaag cgaacaatac ttgcgttatg cctgctgcct
      121 ctttcctttt cgtcattcgc ggcaaccacg gatttagggc cgaaaggaac attgaaattc
      181 acccttaaaa tcagccaggg tgcttgcgaa ctggtgaaag acagcgttga ggttgacatg
      241 ggcaccgcgg ttctcaaaaa gccggtaaga atcggaacgg aaattaatcc cactcctttt
      301 tccatcggcc tgaaaaactg tagcgaagtg gtcagagcct acgtgactat ggacggtaca
      361 cccaatgcag acgactcaaa tctttttgcg ctggatgcgg gtggcgctac aggcgttggg
      421 ctgaaaatca aaaccgctgc tggagtacaa cagttcccgg aaaatacaac gccgacaccg
      481 gttgagtttg caattaacgg tgatggcgca catcaactga actatatcgc cagctacgtg
      541 cctattcgcg ccgatgcaac tattggtcgc gcagatgcaa cggtcgactt tactgtacag
      601 tacgaataat ttcacatctt aagggccagt taactggccc tttttactgc tttttgtaac
      661 gttaaagtct cttttcaaac ttgcattttt gtaaatttgt gcttcatgca cactctttcc
      721 ccacactttt tcgctttgct gtggtctact tattcgcgcg tgtagatttt acttatctga
      781 ctacctccgc acttttcccc tgccgggcct gaaaagccgc taagcagggt gttatcacct
      841 gtttgtccag ggtttgtttg catgagatac atcaaatcga ttacacagca gaagctgagc
      901 tttttgcttg caatctatat tggccttttt atgaatggcg cggtttttta tcgccgcttc
      961 ggcagctatg cgcacgattt taccgtctgg aaaggcgttt ctgctgttgt tgaactggcc
     1021 gccaccgtac tggtgacctt ctttttacta cgtcttctat cgctgtttgg ccgccgcagc
     1081 tggcgtattc tggcgtcgct ggtggtgctc ttttccgcag gtgccagcta ttacatgacc
     1141 ttccttaatg tggtcattgg ttatggcatc atcgcttccg tcatgaccac cgatatcgac
     1201 ctgtcaaaag aagttgttgg tctgaacttt attctctggt taatcgccgt tagtgcattg
     1261 cctcttatcc ttatctggaa taaccgctgt cgctacacct tgctccgaca actgcgaacc
     1321 ccagggcagc gtattcgcag cctggcggtc gtcgtactgg cgggtattat ggtttgggca
     1381 ccgattcgtt tgctggatat ccagcagaag aaagtggaga gggcgaccgg cgttgatttg
     1441 ccgagttatg gcggtgtcgt agcgaactct tatctgccat caaactggct ttctgcgttg
     1501 gggctgtatg cctgggcgcg ggtcgatgaa tcttccgata ataattcatt gcttaatccg
     1561 gcgaagaagt ttacatacca ggctccacaa aacgttgatg atacttatgt cgtgtttatc
     1621 atcggtgaaa ccacgcgttg ggaccatatg ggtattttcg gctatgagcg taataccacg
     1681 ccgaaactgg cccaggagaa aaatctggcg gcgttccgtg gttactcctg tgataccgca
     1741 accaaactct cactgcgttg catgtttgta cgtcaggggg gcgcggaaga taatccgcag
     1801 cgcacattaa aagaacagaa cattttcgcg gttctgaagc agttaggatt cagttctgac
     1861 ctctacgcta tgcagagcga aatgtggttc tacagcaaca cgatggcgga caacattgct
     1921 tatcgtgagc agattggtgc ggagccacgt aatcgtggca agccggtaga tgatatgttg
     1981 ctggtagacg aaatgcagca atcgctaggg cgcaacccgg atggtaagca tctgatcatt
     2041 ctgcatacca aaggttcgca ttttaactac acccagcgtt atccgcgtag cttcgcgcag
     2101 tggaagccgg aatgtattgg tgttgatagc ggctgtacca aagcgcagat gatcaactcc
     2161 tatgacaact cggtgaccta tgtggatcac tttatctcca gcgtgattga tcaggttcgc
     2221 gataagaaag cgattgtgtt ctacgcagct gaccacggtg agtcaattaa tgaacgcgag
     2281 cacctgcacg gcacgccgcg tgaattggca ccgccggagc agttccgcgt accgatgatg
     2341 gtctggatgt cagataaata tctggaaaat ccggccaatg cgcaggcatt tgcgcagctg
     2401 aaaaaagaag ccgacatgaa agtgccacgc cgtcacgtag agctgtacga taccatcatg
     2461 ggttgtcttg gctatacttc accggatggt ggaattaacg aaaacaacaa ctggtgtcac
     2521 atcccgcagg caaaagaggc agcggctaac taagcggcct gctgactttc tcgccgatca
     2581 aaaggcattt tgctattaag ggattgacga gggcgtatct gcgcagtaag atgcgccccg
     2641 cattcggtga ttggcgcagc ctggtagcgc acttcgttcg ggacgaaggg gtcggaggtt
     2701 cgaatcctct atcaccgacc aaattcgaaa agcctgctca gcgagcaggc ttttttgcat
     2761 ttgaagccca tgaggatgag aacctccggg ggctgggagg ttcgactcga cgtaggcctg
     2821 ataagacgcg ccagcgtcgc atcaggcaac ggcaccgcac ttattgtcgg atgcggcgcg
     2881 accgccttat tcgaccaaca caccacacat tttcctgcca actttgcgcc ccatatctca
     2941 ctatccatta acggatttgt gacagaaacc attgtctttt tttatatttg cttaaatctt
     3001 tttttgcgtt acttatggct ggcgctggca ttttccgctg tgcgttttag cgttcatggt
     3061 attaatcgct cagatatcca ccttttcctc agattttttt cattaaatgc ataaaactta
     3121 atcaccaaat gttgctaaat attaagctga ttgttctggt tatgttggag tagcacaaat
     3181 ttctctgctg caaatagctg tttgaagttt tttaatcttt gtttgtgggt tctcaccgtt
     3241 ggtgtaaatc ccggttggct gtattgacgt tttcacattc tgttgacaga ttgtaggtca
     3301 cgaggggcat tttatggagg atccgcactg ttacactgat gttaattagt acggcatccc
     3361 cacctcataa cgttgacccg accgggcaaa aaacaaaaaa ggtcaggtag cgacaaccca
     3421 ctgcaaaggg ttaaaacaac aaacatcaca attggagcag aataatgcgt atttccttga
     3481 aaaagtcagg gatgctgaag cttggtctca gcctggtggc tatgaccgtc gcagcaagtg
     3541 ttcaggctaa aactctggtt tattgctcag aaggctctcc ggaagggttt aacccgcagc
     3601 tgtttacctc cggcaccacc tatgacgcct cttccgtacc gctttataac cgcctggttg
     3661 aatttaaaat cggcaccact gaagtgatac cgggcctcgc tgaaaagtgg gaagtcagct
     3721 aagacggtaa aatctatacc ttccatctgc gtaaaggtgt gaagtggcac gacaataaag
     3781 aattcaaacc gacgcgtgaa ctgaacgccg atgacgtggt gttctcgttc gaccgtcaga
     3841 aaaacgcgca aaacccgtac cataaagtct ctggcggcag ctacgaatac ttcgaaggca
     3901 tgggcttgcc ggagctgatc agtgaagtga aaaaggtgga cgacaacaca gttcagttcg
     3961 tactgactcg cccggaagcg ccgttcctcg ctgatctggc aatggacttc gcctctattc
     4021 tgtcaaaaga atatgctgat gcgatgatga aagccggtac accggaaaaa ctggacctca
     4081 acccaatcgg aaccggtccg ttccagttac agcagtacca aaaagattcc cgtatccgct
     4141 acaaagcgtt tgatggctac tggggcacca aaccgcagat cgatacgctg gtcttctcta
     4201 ttacccctga tgcttccgtg cgttacgcga aattgcagaa gaatgaatgc caggtgatgc
     4261 cgtacccgaa cccggcagat atcgctcgca tgaagcagga taaatccatc aacctgatgg
     4321 aaatgccggg gctgaacgtc ggctacctct cgtataacgt gcagaaaaaa ccgctggatg
     4381 acgtgaaagt tcgccaggca ctgacctacg cggtgaacaa agacgcgatc atcaaagcgg
     4441 tttatcaggg cgcgggtgta tcagcgaaaa acctgatccc gccaaccatg tggggctata
     4501 acgacgacgt tcaggattac acctacgatc ctgaaaaagc gaaagccttg ctgaaagaag
     4561 cgggtctgga aaaaggtttc tccatcgacc tgtgggcgat gccggtacaa cgtccgtata
     4621 acccgaacgc tcgctgcatg gcggagatga ttcaggcaga ctgggcgaaa gtgggcgtgc
     4681 aggccaaaat cgtcacctac gaatggggtg agtacctcaa gcgtgcgaaa gatggcgaac
     4741 atcagacggt aatgatgggc tggactggcg ataacgggga cccggataac ttcttcgcca
     4801 ccctgttcag ctgcgccgcc tctgaacaag gttctaacta ctcaaaatgg tgctacaaac
     4861 cgtttgaaga tctgattcaa ccggcgcgtg ctaccgacga ccataacaaa cgcgttgaac
     4921 tgtacaagca atcgcaggtg gtgatgcacg atcaggctcc ggcactgatc atcgctcact
     4981 ccaccgtgtt tgaaccggta cgcaaagaag tcaaaggcta tgtggttgat ccattaggca
     5041 aacatcactt cgaaaatgtc tctatcgaat aattaaaagc catacaagac tgatggtaaa
     5101 ggcaaaactg cctgatgtgc tccgcttatc aggcctacga aaattctgca atgtattgaa
     5161 tttgcacgat tttgtaggcc ggataaggcg ttaacgccgc atccggcata aacaaagcgc
     5221 actttgtcaa caatctgtat acccggtggc gctgtgcctc tgcgtgcagc gccatccggc
     5281 agcaatactt cattctctgt ccgacaaggg cgggaattga tttgtgagca atacagacac
     5341 gcagttccag gctgcgggtc actatagaga atccgggtta tgttgcagtt tattctccga
     5401 cgtttgggac tcgtcatccc cacgtttatc ggtattaccc ttctcacatt tgcctttgtc
     5461 cacatgatcc cgggcgatcc ggtgatgatc atggcaggcg aacgtgggat ctcccctgag
     5521 cgtcacgcgc agctgctggc tgaactcggc ttagataaac cgatgtggca gcagtatctc
     5581 cattacattt ggggcgtaat gcacggcgat ctaggcattt caatgaaaag ccgaattcca
     5641 gtatgggaag agttcgtgcc gcgctttcag gccacgctgg aacttggcgt ctgcgcgatg
     5701 atttttgcta cggcagtcgg tattccggtt ggtgtgctgg ctgcggttaa acgcggttcc
     5761 attttcgatc acactgcagt tggcctggcg ctgaccggct actcgatgcc gatcttctgg
     5821 tggggcatga tgctgatcat gctggtttcg gtgcactgga acctgacgcc cgcctccggt
     5881 cgcgtgagcg atatggtgtt cctcgatgac tccaatccgt taaccggttt tatgctaatc
     5941 gacaccgcca tctggggtga agacggcaac tttatcgatg ccgtcgccca tatgatcttg
     6001 cctgccattg tgctgggtac tattccgctg gcggtcattg tgcgtatgac acgctcctcg
     6061 atgctggaag tgctgggcga ggattacatc cgcaccgcgc gcgccaaagg gctaacccgc
     6121 atgcgggtga ttatcgtcca tgcgctgcgt aacgcgatgc tgccggtggt gaccgttatc
     6181 ggcctgcagg tgggaacatt gctggcgggg gcgattctga ccgaaaccat cttctcgtgg
     6241 cccggtctgg gacgctggtt gattgacgca ctgcaacgcc gcgactatcc ggtagtgcag
     6301 ggcggcgtat tgctggtggc gacgatgatt atcctcgtca acttgctggt cgatctgctg
     6361 tacggcgtgg tgaacccgcg tattcgtcat aagaagtaag gggacatcat gtcacaggtt
     6421 actgaaaata aagtgattag cgcaccggtg ccgatgaccc cgttacagga gttctggcac
     6481 tattttaaac gcaacaaagg cgcggtcgtc gggctggttt acgtcgtcat cgtgctgttc
     6541 atcgcgatct ttgccaactg gattgcaccc tataacccgg cggaacagtt ccgcgatgca
     6601 ctgctcgccc cgccagcctg gcaggaaggc ggcagcatgg cgcacttgct gggcaccgat
     6661 gacgtaggcc gtgatatgct gtcgcgcctg atgtacggtg cgcgcctgtc gctgctggtt
     6721 ggctgtctgg tagttgtgtt atcgctgatt atgggcgtta ttctcggcct gatcgccggt
     6781 tactttggcg gcctagtcga taacatcatt atgcgcgtgg tcgatatcat gctggcgctg
     6841 ccaagtctgc tgctggcgct ggtgctggtg gcaattttcg gcccgtcgat tggtaacgcc
     6901 gcgctggcac tgaccttcgt tgccttgccg cactatgtgc gcttaacccg cgccgccgtg
     6961 ctggtggaag ttaaccgcga ttacgtcacc gcgtctcgcg tggcgggtgc cggggcgatg
     7021 cgtcagatgt ttattaacat cttcccgaac tgccttgcgc cgctgattgt tcaggcgtcg
     7081 ctcggtttct ctaacgccat tctcgatatg gctgctcttg gtttcctcgg catgggggca
     7141 cagccgccaa cgccggagtg gggcaccatg ctctccgacg tgttgcagtt cgcgcaaagc
     7201 gcctggtggg tcgtgacctt cccgggtctg gcgatcctgc tgacggtgct ggcatttaac
     7261 ctgatgggtg acggtctgcg tgacgcgctc gatcccaaac tgaagcagta agaggttcga
     7321 gatggcgtta ttaaatgtag ataaattatc ggtgcatttc ggcgacgaaa gcgcaccgtt
     7381 ccgcgccgta gaccgcatca gctacagcgt aaaacagggc gaagtggttg ggattgtggg
     7441 tgagtccggc tccggtaagt cggtcagttc cctggcgatt atggggctga ttgattatcc
     7501 gggccgcgta atggcggaaa aactggtgtt taacggtcag gatttgcagc gtatatcaga
     7561 aaaagagcgc cgcaacctgg tgggtgccga agtggcgatg atcttccagg acccgatgac
     7621 cagccttaac ccgtgctaca ccgtgggttt ccagatcatg gaagcgatta aggtgcatca
     7681 gggcggcaac aaaagtaccc gccgtcagcg ggcgattgac ctgctgaatc aggtcggtat
     7741 tcccgatccg gcttcgcgtc tggatgttta cccgcatcag ctttccggcg gcatgagcca
     7801 gcgcgtgatg atcgccatgg cgattgcctg tcgaccaaaa ctgctgattg ccgatgagcc
     7861 gaccaccgcg ctggacgtga ccattcaggc gcaaatcatc gaactactgc tggagctaca
     7921 gcagaaagag aacatggcgc tggtgttgat tacccatgac ctggcgctgg tggcggaagc
     7981 tgcgcataaa atcattgtga tgtatgcagg ccaggtggtg gaaacgggcg atgcgcacgc
     8041 catcttccat gcgccgcgtc acccgtatac tcaggcattg ctgcgtgcgc tgccagaatt
     8101 tgctcaggac aaagaacgtc tggcgtcgtt gccaggtgtc gttcccggca agtacgaccg
     8161 cccgaacggc tgcctgctta acccgcgctg cccctatgcc actgacagat gtcgcgctga
     8221 agaaccggcg ctgaatatgc tcgctgacgg gcgtcagtcc aaatgccatt acccacttga
     8281 tgatgccggg aggccgacac tatgagtacg caagaggcca ccctgcaaca accgctgttg
     8341 caggctatcg acctgaaaaa acattatccg gtgaagaaag gcatgttcgc gccggaacgt
     8401 ctggttaaag cgctggatgg cgtttcgttt aaccttgaac gtggcaaaac gctggcagta
     8461 gtgggcgaat ctggctgcgg taaatcgacc ctcggtcggt tgctgacgat gattgaaacg
     8521 cccaccggtg gcgagctgta ttaccagggg caggatctgc ttaagcacga tccgcaggcg
     8581 cagaagctgc gtcggcagaa aatccagatc gtcttccaga acccttacgg ttcgctgaat
     8641 ccgcgtaaaa aagtcgggca aattcttgaa gagccgctgc tgatcaacac cagcttaagc
     8701 aaagaacagc gtcgggaaaa agccctgtcg atgatggcga aagtcggcct gaaaaccgag
     8761 cactatgacc gctatccgca tatgttctcc ggcggtcagc gtcagcgtat cgccatcgcc
     8821 cgtggtctga tgctcgaccc ggatgtggtg attgccgatg aaccggtttc cgcgctggat
     8881 gtttcagtgc gcgcgcaggt gctgaatctg atgatggatt tgcagcagga gttggggctg
     8941 tcttatgtct ttatctccca cgacctgtcg gtggtggagc acattgctga tgaagtgatg
     9001 gtgatgtacc tgggccgctg cgtggagaag ggaacgaaag accaaatctt caataacccg
     9061 cgccatccgt acactcaggc gctactttcc gcgacgccgc gcctgaaccc ggacgatcgc
     9121 cgcgagcgca tcaagctcag cggtgaacta ccaagcccac tgaatccacc gccgggttgc
     9181 gccttcaacg cccgctgtcg tcggcgcttc ggcccctgca cccagttgca gccgcagcta
     9241 aaagactacg gcggtcaact ggtagcttgt tttgctgttg atcaggatga aaatccgcag
     9301 cgttaactca caaaaccggg gccatgcgct ccggtttact caatcagctt caacagtggc
     9361 gacaagcata gcaaaatgcc gtaaagcaga attagccagg ctttaaatcc gcgaagtttt
     9421 tccagttgtg cgactttata gatcaggaaa aacggaatga ggcacgacac aatgccatat
     9481 aacgggcttc ccaactgaaa gaacaccagc accgatacac gaaacgaaac ccaaatcgtc
     9541 aacgtaatga cgataaaagc gcagatcgcc agagtcagca tgcgtgagtt aattttcttg
     9601 gtatcaataa ttcggcttaa cagattgaga ataatgcctt taatagcctc gtggaaaccg
     9661 agataaatgc caaagaatgc ggtcagtacg gcaaagatat tcagcaccgt agaggtgata
     9721 tgaatgatat gtccagggat cacctgcgcg gccagtgcca gtgccgagat attttgttca
     9781 aaggcagaaa cggcttcttc gtggctaatt gagaaggtaa acgaaaaggc aaaaaacagg
     9841 atcaccgcga tgagcgtgat ataactaatt cggtgggtac gcagcgcgag ccgggttgcc
     9901 agtactttat ctgcttcccg tttacgatag gcaatattca ttgggttaag tacctgaata
     9961 aataccgcag aaaagaagca aaatggaatt gtaagcagga catcgcggaa aaagacggag
    10021 gcttgcggga aggcggttat attggcgaaa ttccagtgcg ggatcatcgc aaaaccgaac
    10081 acgacaataa tccctacttt gaccaccacc attggcccgg aaatcttaaa cagtaatcgt
    10141 tcaccaccag acgcaatcgc taccagtacg gcgaaaatag cgactttata aagtagagat
    10201 tgtgaaagat cggcatcggt taaaccgaag gttttcaggt acgaggcgct gtcgaaaacc
    10261 acggagagag agtagataaa aatcccgtgg ataatcatca aaaagtagat aaccccgagg
    10321 aaaattcccc agttcttccc caggtaatga ctgataatat cggtgtagtc attacaggaa
    10381 tcgctttcag aaagggtttt taaataaatg tcctgcacta cccaggtggc aggataagca
    10441 atgatcgctg cggtaataaa tacccaaatt cccttcaagc caatttgtac tggcatcagc
    10501 acggttccgg caccaatcgc catgcctatg cataataaaa cccagccgaa gtcgtagcgt
    10561 gtaaacggca atttctgatt gtgtttcgat agtgtgttgt gctgcataaa gtcctgtcca
    10621 gaaagaagtc ggcctcgcaa aaatgaataa acccagtttc tcttgcgcaa caggtggaga
    10681 agtcgaatac gcagtatgta aaaaatgagg caacagattc actggggagg tcgcgaggag
    10741 gcgcaattct acaaaattgc gcatttcact accagtctgg agtcagcccg gatatgaact
    10801 gcaactattg caaataaaga aattaagctg gaaggcatcg cgcaatgagg aagattgccg
    10861 ggtgctgaca gtaaaaagca atcgtaaaat cagcggcaag ccgaaagggt tccggtgagg
    10921 cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggagagag aaaacccccg
    10981 cacgttgcag gtatgcacct gacaacatca cgggggctaa tcttgaaccc aaacaactca
    11041 agaatagccg cgaaacgttg gcattacaac acaggcggct atatgacgct cgcagaactg
    11101 ggcatggcct tctggcatga tttagcggct ccggtcattg ctggcattct tgccagtatg
    11161 atcgtgaact ggctgaataa gcggaagtaa cgtgtcatgc gggcgtcagg ctgccgtaat
    11221 ggcaatttgc gcccggatca ggccgcagag gttaaactct gcggcctttt tcgtgcttac
    11281 tgcgggtaag gcacccagtc gccgccgttc aggcgaacgt acggtttatc ctggtattga
    11341 ataactactg catttgagtt ctcggagacc ggtgctgttt gtggcaaccc actggtgagt
    11401 tttttccagt caacattgtc ttcggtgaaa atcttgccat caagaacgcg aaccaccaga
    11461 tcggagatag ccaggaagct gctcggttgg tcgatgacaa tcggtgcccc ctgatgtggt
    11521 gccttcatgc cgaagaattt caccccaacg gggacgtctg tgatagacgg gctagggata
    11581 tcacgtaggc cagatacctg cattctgtcg cccttcagcg cgccgccgtg ttccggcacc
    11641 acgaccacca tcactttacg acccgatttc tccagttcag taaagaaggc gtccagttca
    11701 tcaaagaatt tctgcgcccg cgctttgtaa tccgctgttt tgctgacccc cggataatgg
    11761 ttgccgtcat gcagtggaag cgtgttgtag aacgtggcac tacggctgtt tttatctttt
    11821 tcggtaacgt ccagccagcg gttaagcacg gtggtatcgt cataaaccgg cgaaccatca
    11881 aagcccagca aaataaccgg cagatttgtt tgatccatca attcagtctg catgccgcca
    11941 ttttcgcgaa cttctttcaa aaaaccgccg aactggccgt tatgtcccat catcaggtgc
    12001 tgggtaaagc ccagtttcga aaggttatca aacagatagc agtcgttatt tgccggttga
    12061 tacagattag tgtgcgaagt ctgcccgcag ctggcgcgca gtaaacggat cgccgccggg
    12121 ccactgtagg aggtggcgga gttaaagttc ttgaactcaa tatcgaaatg cgaccacagt
    12181 ggatgcgaca tcaacccggc ggcttctata tccgaccagg aaagcgaaca gatgttaatc
    12241 accagtagtt caaatggctg agcatcagcg ggcagcgaag acgggaaggt cgatttacgt
    12301 ttcgcctccg cgttatagaa attattcagc caggcgttaa ggttcgccgt tgttggcggt
    12361 gtagtttgtg cgggcatatc acccactacc ggtgcgccac ccgtcgtctc aacggttgcc
    12421 gctgcgttac cacccgtcgt tgttacagtg gtcgtcggtt gtccggctgg ccacaaggag
    12481 aaacttggtc ccgccagggt aagtacgttc agccatagca gtatggcaac cacaaaaacg
    12541 gtaatgcgaa tccattgtga caggaataac caggccacta ataaaacaaa aatggcccca
    12601 atcatctgcc agttaataaa gcgtgtgaca aggtcgatta aataatcggt actgaacccc
    12661 gccacctgcg aaccctggct cattatgctt tccgggccag gcaaccaggt gtcatgccag
    12721 aacaaagcaa agccgatcgg cagggcaatc cagtggcgca agcgatgcag gctgtagcgc
    12781 ggaatgggca tcagcagaaa cgcggcaaac accaaattga ggagcggatg gaagttaaga
    12841 tatcccgccc acaacaggcc gaacttaacc agaaaataga agttccagcc ggaaaggccg
    12901 cgccagtatt gccagaggga agaaggcatg gcggtatttt gcgtaaattg agtcattttt
    12961 tggttgccct ggctttttcc gtgcggcgta atgtcccggc cggtttaaca taacgaggtt
    13021 tagcaaagaa caaacgtaag gtgtcgcgaa tgcgtcgcaa agagtgccgc gccagatagc
    13081 ccagcgggaa aaatatcagt gcgcaaacga caataatttc aatgatatcg ctgatggtca
    13141 tcatgatgag cgctccacag catcatctaa cagtcgcatg ggttctggta ttcgtcgcca
    13201 gtggcgacca tcgtgctcgg cattgatgac cggtttagaa ctttgcgtta aaggcagcgg
    13261 catgccccat tgttctgggg caagcaggcg catctgcacc agctcggcac tgatttgatc
    13321 atcttcaaac cagaccatac ggtttgagaa aatgtcgcca gtaggcaatg ggaaaatatg
    13381 attcaacgcg gtatccagat cgttaatccg acagaatgag agaaacagca ccagccgatt
    13441 accgccaatg gtcatgatat cgccggtacg gttagggcga cacagcgtca gggcttgttc
    13501 aacgcggata cccggtaccg gacgtagggc aaccagaacg cctttaccgt gggcaggtaa
    13561 tagagggtta ttcatcatgt tgttgacggc attacagaac acatcccact tctggaaacc
    13621 acgcagtttg agcggctggg tcattgacag caaggtagtg atatcttccg gcacatagcg
    13681 actaaacttc tgcccttgca cgctttcgat catcgtcaga caacgggaga gtggcgcatt
    13741 ccacggaata accatatttg caccgcaggc caataacaaa cgttcatcgg tggcgcgcag
    13801 gctagcggta ttttcccgca cgaggatttt catcgcacta ccgcgctggc gacgcagggt
    13861 atgaatgctg cgggccagtg gctcgatttg cgcattttgc tgtaaagaaa agaccaccgt
    13921 cgccgcctga gcggtacggg cttcattgaa caggacttcg ttattgttga acagttgcca
    13981 gtgttccgat agcggcggcg caccttccag tacagcaaca ttactcagaa tgcgtttttc
    14041 gtcgctgcgt ggttggatct ccgcctcttc gctttgaact aatgtccaga taccattttg
    14101 ttgctgaacg ctaagctgct gacgggcgct gacccctttt tcgttgcacc agaaggcaat
    14161 atccagcaaa tgttggtcac cctgaaaacg caaactggca agaccaaaaa gtgaacggta
    14221 ttcctcaagc aacaatgaaa attgtttatc gttattattt ccgggattaa ttaccaaaag
    14281 cgaacaatgg gttaacctgc tccatttatt cattttatcc aaccatgagc gaagccgctc
    14341 ggcaggaatg ttttgccatg cgttatttgc gcaaacaaga ataaaaaggt aattatgggg
    14401 atcaatagaa cactgcaaat cacgggtcaa atagtataga cctttttcat gatttagcat
    14461 tgaaaataat tttatttttt ccggcccttg ataatcatct aattgaaaga ttttcgccgg
    14521 atcgctgtcc atgctaatga ccgcgacgtg tgcggtttca gcctgggatg caattgtttg
    14581 attcgccaga ctgatagcat cttcatggcg atcgacgtta aaccaccaga cgccgcctgc
    14641 tggcatatgt cgcagctcat cccataatga tgagataccg atagagaata cagggcccac
    14701 aatgtccctc atgtcaccat tttgtctgat tatcagttta ctagcgaaag ctaagaaata
    14761 aacctaccat tgaaattaaa acttatcaga tttagcatgt gaatcaattt agaaggtcac
    14821 aagacgtgtg attttagtct tttcgttttt aactttttct gttgcgtaac ggaaagtcaa
    14881 aaagtgagca aattcccgtc tcgccgtaaa caataaaccg gcgaaatgct cacggttaga
    14941 attgtctcat gaacggtacg gttatttcat agggatcaag caaaatgaat aacaatgaac
    15001 cagatactct gcctgatccc gcgataggct atatcttcca gaatgatatt gtggcgttaa
    15061 agcaggcatt ttcactgcct gatattgatt atgccgatat ttcccaacgc gaacagttgg
    15121 ccgcggcatt aaaacgctgg ccgttgctgg cagagtttgc gcaacaaaag taggggattg
    15181 gtgaatggcc gtactgggat tgcagggggt gcggggaggc gtggggacaa caaccatcac
    15241 cgccgcatta gcctggtcat tacaaatgtt gggagaaaat gtcctggtgg tcgatgcctg
    15301 cccggacaac ttgttgcgcc tgtcatttaa tgttgatttt acccaccgtc agggctgggc
    15361 cagagcgatg ctggatggcc aggactggcg tgacgctggg ttgcgctaca cctcgcagct
    15421 cgatttgctg ccttttggtc agttatccat tgaagaacaa gaaaatccac agcacaggca
    15481 aacccggctg agcgatattt gctccggctt acagcaacta aaagccagcg ggcgttacca
    15541 gtggatttta atcgacttac cgcgtgatgc ctcgcagata acccaccagc tgctgagttt
    15601 gtgcgatcac tcgctggcaa tcgtcaatgt ggatgccaac tgccatatcc gactgcatca
    15661 gcaagcgctg ccggatggcg cacatatttt gattaatgac ttccgtattg gcagtcaggt
    15721 tcaggacgat atttaccagc tttggttgca aagccagcgc cgattactgc cgatgctcat
    15781 tcatcgtgat gaagcgatgg ctgaatgcct ggcggctaag caaccagtag gtgaatatcg
    15841 cagtgatgcg ctggcggctg aagagatact gacgctggcg aactggtgcc tgttgaacta
    15901 ctccgggctg aaaacgccag tcgggagtgc atcatgagta tcctgacccg gtggttgctt
    15961 atcccgccgg tcaacgcgcg gcttatcggg ggttatcgcg attatcgtcg tcacggtgcg
    16021 tcggctttca gcgcgacgct cggctgtttc tggatgatcc tggcctggat ttttattccg
    16081 ctggagcacc cgcgctggca gcgtattcgc gcagaacata aaaacctgta tccgcatatc
    16141 aacgcctcgc gtccgcgtcc gctggacccg gtccgttatc tcattcaaac atgctggtta
    16201 ctgatcggta catcgcgcaa agaaacgccg aaaccgcgca ggcgggcatt ttcaggtctg
    16261 cagaatattc gtggacgtta ccatcaatgg atgaacgagc tgcctgagcg cgttagccat
    16321 aaaacacagc atcttgatga gaaaaaagag ctcggtcatt tgagtgccgg ggcgcggcgg
    16381 ttgatcctcg gtatcatcgt caccttctcg ctgattctgg cgttaatctg cgttactcaa
    16441 ccgtttaacc cgctggcgca gtttatcttc ctgatgctgc tgtggggtgt agcgctgatc
    16501 gtacggcgga tgccggggcg cttctcggcg ctaatgttga ttgtgctgtc gctgaccgtt
    16561 tcttgccgtt atatctggtg gcgttacacc tctacgctga actgggacga tccggtcagc
    16621 ctggtgtgcg ggcttattct gctcttcgct gaaacgtacg cgtggattgt gctggtgctc
    16681 ggctacttcc aggtcgtatg gccgctgaat cgtcagccgg tgccattgcc gaaagatatg
    16741 tcgctgtggc cgtcggtgga tatctttgtc ccgacttaca acgaagatct caacgtggtg
    16801 aaaaatacca tttacgcctc gctgggtatc gactggccga aagacaaact gaacatctgg
    16861 atcctcgatg acggcggcag ggaagagttt cgccagtttg cgcaaaacgt gggggtgaaa
    16921 tatatcgccc gcaccactca tgaacatgcg aaagcgggca acatcaacaa tgcgctgaaa
    16981 tatgccaaag gcgagttcgt atcgattttc gactgcgacc acgtaccaac gcgatcgttc
    17041 ctgcaaatga ccatgggctg gttcctgaaa gagaaacagc tggcgatgat gcagacgccg
    17101 caccacttct tctcgccgga cccgtttgaa cgcaacctgg ggcgttttcg taaaacgccg
    17161 aacgaaggca cgctgttcta tggtctggtg caggatggca acgatatgtg ggacgccact
    17221 ttcttctgcg gttcctgtgc agtaattcgt cgcaaaccat tggatgaaat tggcggtatt
    17281 gctgtcgaaa cggtaaccga agatgcgcac acgtctctgc gcctgcaccg tcgtggctac
    17341 acctctgcat atatgcgtat tccgcaggcg gcggggctgg cgaccgaaag tctgtcggcg
    17401 catatcggtc agcgtattcg ctgggcacgc gggatggtgc aaatcttccg tctcgataac
    17461 ccgcttaccg gtaaagggct gaaattcgcc cagcggctgt gctacgtcaa cgccatgttc
    17521 cacttcttgt cgggcattcc acgactgatc ttcctgactg cgccgctggc gttcctgctg
    17581 cttcatgcct acatcatcta tgcgccagcg ttgatgatcg ccctgttcgt gctgccgcat
    17641 atgatccatg ccagcctgac caactcgaag atccagggca aatatcgcca ctctttctgg
    17701 agtgaaatct acgaaacggt gctggcgtgg tatatcgcac caccgacgct ggtggcgctg
    17761 attaacccgc acaaaggcaa atttaacgtc accgccaaag gtggactggt ggaagaagag
    17821 tacgtcgact gggtgatctc gcggccctac atcttccttg ttctgctcaa cctggtgggt
    17881 gttgcggtcg gcatctggcg ctacttctat ggcccgccaa ccgagatgct caccgtggtc
    17941 gtcagtatgg tgtgggtgtt ctacaacctg attgttcttg gcggcgcagt tgcggtatcg
    18001 gtagaaagca aacaggtacg ccgatcgcac cgcgtggaga tgacgatgcc cgcggcaatt
    18061 gcccgcgaag atggtcacct cttttcgtgt accgttcagg atttctccga cggtggtttg
    18121 gggatcaaga tcaacggtca ggcgcagatt ctggaagggc agaaagtgaa tctgttgctt
    18181 aaacgcggtc agcaggaata cgtcttcccg acccaggtgg cgcgcgtgat gggtaatgaa
    18241 gttgggctga aattaatgcc gctcaccacc cagcaacata tcgattttgt gcagtgtacg
    18301 tttgcccgtg cggatacatg ggcgctctgg caggacagct acccggaaga taagccgctg
    18361 gaaagtctgc tggatattct gaagctcggc ttccgtggct accgccatct ggcggagttt
    18421 gcgccttctt cggtgaaggg catattccgt gtgctgactt ctctggtttc ctgggttgta
    18481 tcgtttattc tgcgccgccc ggagcggagc gaaacggcac aaccatcgga tcaggctttg
    18541 gctcaacaat gatgataacg cgatgaaaag aaaactattc tggatttgtg cagtggctat
    18601 ggggatgagt gcgttcccct ctttcatgac gcaggcgacg ccagcaacgc aaccactgat
    18661 caatgctgag ccagctgtag ccgcccagac ggaacaaaat ccgcaggtgg ggcaagtgat
    18721 gccgggcgtg cagggcgctg atgcgccagt cgtggcgcag aacggtcctt cgcgtgatgt
    18781 gaagctgacc tttgcgcaaa ttgcgcctcc gccgggcagc atggtgctac gtggcattaa
    18841 cccgaacggc agcattgagt ttggtatgcg cagcgatgaa gtggtgacga aggcgatgct
    18901 caacctcgaa tacatgccat cgccatcgtt actgcctgtc cagtcgcagt taaggtttat
    18961 ctcaatgatg agctgatggg cgtactgcca gtgaccaaag aacagttggg taaaaaaacg
    19021 ctggcgcaaa tgcccattaa cccactgttt attaccgact tcaaccgtgt gcggctggag
    19081 tttgtcggcc attatcagga cgtgtgcgaa aacccggcca gaaccacgct ttggctggat
    19141 gttgggcgga gcagtggact ggatctgacc tatcagaccc tgaatgtgaa gaatgacctg
    19201 tcacacttcc cggtgccatt ctttgacccg cgcgataacc gtactaacac cttgccgatg
    19261 gtctttgcgg gtgcgccgga tgttgggctg caacaagcat ctgccattgt cgcctcgtgg
    19321 tttggttcgc gttctggctg gcgtgggcag aacttcccgg tgctctataa ccaactgccg
    19381 gatcgtaatg ccattgtctt tgcaaccaac gacaaacggc cggacttcct gcgcgatcat
    19441 ccggcggtaa aagccccggt gattgagatg atcaaccatc cgcagaatcc ttacgtcaaa
    19501 ctgctggtgg tgtttggtcg tgacgacaaa gacctgttgc aggcagcgaa aggtatcgct
    19561 cagggtaaca ttctgttccg tggtgaaagc gtggtagtga atgaagtgaa accgctgcta
    19621 ccgcgtaagc cgtacgatgc gccgaactgg gtacgtaccg atcgtccggt cacatttggc
    19681 gaactgaaaa cctatgaaga acagttacaa tccagcggtc ttgagccagc agcgattaac
    19741 gtttcgctaa acctgccgcc ggatctctac ctgatgcgca gtaccggcat tgatatggat
    19801 attaattacc gctacaccat gccgccggtg aaagacagtt cgcggatgga tatcagcctg
    19861 aataaccagt tcctgcaatc cttcaacctg agcagcaaac aggaggcgaa ccgcctgctg
    19921 ctgcggattc cggtattaca aggtttgctg gatggcaaaa cagatgtctc tattccggcg
    19981 ctgaaactgg gcgcgaccaa ccagctgcgc ttcgactttg agtatatgaa cccgatgccg
    20041 ggcggttcgg tggataactg tattaccttc cagccggtgc agaatcatgt ggtgattggt
    20101 gacgactcca ccatcgactt ctcgaagtat taccacttca tcccgatgcc ggatctacgc
    20161 gcctttgcta acgcgggctt cccattcagc cggatggcgg atctgtcgca aaccatcacc
    20221 gtgatgccga aagcgcctaa cgaagcacag atggaaacgt tgctgaatac tgttggtttt
    20281 atcggcgcac agacgggctt cccggcgatt aatctgacgg tgaccgatga tggcagcacc
    20341 attcagggca aagatgccga catcatgatc atcggtggta tcccggacaa actgaaagac
    20401 gataagcaga tcgacctatt ggtgcaggcg accgaaagct gggtgaaaac accgatgcgc
    20461 cagaccccgt tccccggcat tgtgccggac gagagcgatc gcgcggcaga aacccggtca
    20521 acgctgacct cttccggtgc gatggcggcg gtgattggct tccagtcgcc gtataacgac
    20581 cagcgcagcg tgattgcgct gttggcagat agcccacgcg gttatgaaat gcttaacgat
    20641 gcggtgaacg atagcggcaa acgcgccacc atgttcggtt cggtcgcggt gatccgcgag
    20701 tccggtatca acagcctacg tgttggcgac gtttattacg taggtcatct gccgtggttc
    20761 gagcgcgtgt ggtatgcgct ggcaaaccat ccgattctgc tggcggtgct ggcggctatc
    20821 agtgtgatat tgctggcatg ggtactgtgg cgtctgctgc gaattattag tcgtcgtcgt
    20881 cttaacccgg ataacgagta attgaagatg aatgtgttgc gtagtggaat cgtgacgatg
    20941 ctgttgttgg ctgcctttag tgttcaggca gcctgtacct ggcctgcctg ggagcagttt
    21001 aaaaaggatt acatcagtca ggaagggcgc gtcatcgacc ccagcgacgc gcgcaaaatc
    21061 accacctccg aagggcaaag ttacggtatg ttctttgccc tggcggctaa cgaccgtgca
    21121 gctttcgata atattctcga ctggacgcag aacaatctcg ctcagggttc tttaaaagaa
    21181 cgtttgcccg cctggctgtg gggcaagaaa gagaacagta agtgggaagt gctggacagc
    21241 aattcggcct ccgatggtga tgtctggatg gcctggtcgt tgctggaggc ggggcgtttg
    21301 tggaaagagc agcgttatac cgacatcggc agcgcgttgc taaaacgtat cgcgcgggag
    21361 gaagtggtga cggtgcctgg gctgggttcc atgttgttac cgggcaaagt gggttttgct
    21421 gaggataaca gctggcgttt taaccccagc tacctgccgc cgacgctggc gcagtatttc
    21481 acccgctttg gcgcgccgtg gactacgctg cgcgaaacca atcaacgttt attgctggaa
    21541 accgccccga aaggcttttc gccagactgg gtgcgttatg agaaagacaa aggctggcag
    21601 ctaaaagccg aaaaaacatt gatcagcagc tacgacgcca tccgcgttta catgtgggta
    21661 ggcatgatgc ctgacagcga tccgcagaaa gcgcggatgc tcaaccggtt taaaccgatg
    21721 gcgacattca ctgagaaaaa cggttatccg ccggaaaaag tggatgtggc tacggggaaa
    21781 gcgcagggta aaggaccagt cggtttttct gccgccatgc tgcccttttt acaaaaccgc
    21841 gatgcgcagg ccgttcagcg tcagcgcgtg gccgataact ttcccggcag cgatgcctat
    21901 tacaactatg tgctgaccct gtttggacaa ggctgggatc aacaccgttt ccgcttctcg
    21961 acaaaaggtg agttattacc tgactggggc caggaatgcg caaattcaca ctaaacatat
    22021 tcacgctttc cctcggtctg gccgtcatgc cgatggtcga ggcagcacca accgctcagc
    22081 aacagttgct ggagcaagtt cggttaggcg aagcgaccca tcgtgaagat ctggtgcaac
    22141 agtcgttata tcggctggaa cttattgatc cgaataaccc ggacgtcgtt gccgcccgtt
    22201 tccgttcttt gttacgttag ggcgatattg atggcgcgca aaaacagctc gatcggctgt
    22261 cgcagttagc gccgagttca aatgcgtata aatcgtcgcg gactacgatg ctactttcca
    22321 cgccggatgg tcgtcaggca ctgcaacagg cacgattgca ggcgacgacc ggtcatgcag
    22381 aagaagctgt ggcgagttac aacaaactgt tcaacggtgc gccgccggaa ggtgacattg
    22441 ctgtcgagta ctggagtacg gtggcgaaaa ttccggctcg ccgtggcgaa gcgattaatc
    22501 agttaaaacg catcaatgcg gatgcaccgg gcaatacggg cctgcaaaac aatctggcgc
    22561 tattgctgtt tagtagcgat cgccgtgacg aaggttttgc cgtcctggaa cagatggcaa
    22621 aatcgaacgc cgggcgcgaa ggggcctcta aaatctggta cgggcagatt aaagacatgc
    22681 ccgtcagtga tgccagtgtg tcggcgctga aaaaatatct ctcgatcttt agtgatggcg
    22741 atagcgtggc ggctgcgcaa tcgcaactgg cagaacagca aaaacagctg gccgatcctg
    22801 ctttccgcgc tcgtgcgcaa ggtttagcgg cggtggactc tggtatggcg ggtaaagcca
    22861 ttcccgaact acaacaggcg gtgcgggcga acccgaaaga cagtgaagct ctgggggcgc
    22921 tgggccaggc gtattctcag aaaggcgatc gcgccaatgc agtggcgaat ctggaaaaag
    22981 ccctcgcact ggacccgcac agcagcaaca acgacaaatg gaacagtctg ctgaaagtaa
    23041 accgctactg gctggcgatc cagcagggcg atgctgcgct gaaagccaat aatcctgacc
    23101 gggcagaacg cctgttccag caggcgcgta atgtcgataa caccgacagt tatgcagtgc
    23161 tggggctggg cgatgtggcg atggcgcgaa aagattatcc cgccgccgaa cgttattatc
    23221 agcagacctt gcgtatggac agcggcaaca ctaacgccgt gcgcgggctg gcaaatattt
    23281 accgccagca atcgccagaa aaagctgaag cgtttatcgc ctcgctctct gccagtcagc
    23341 ggcgtagcat tgatgatatc gaacgcagcc tgcaaaacga ccgtctggca cagcaggcag
    23401 aggcactgga aaaccagggc aaatgggcgc cggcggcagc acttcagcgg caacgactgg
    23461 cgctggaccc cggcagcgta tggattactt accgactttc gcaggatctc tggcaggccg
    23521 gacaacgcag ccaggccgat acgttaatgc gcaatctggc gcagcagaag tcgaacgacc
    23581 cggagcaggt ttacgcttac gggctgtacc tctctggtca tgaccaggac agagcggcgc
    23641 tggcgcatat caacagcctg ccgcgagcgc agtggaacag caatattcag gagctggtta
    23701 atcgactgca aagcgatcag gtgctggaaa ccgctaatcg cctgcgagaa agcggcaaag
    23761 aagctgaagc ggaagcgatg ctgcgtcagc aaccaccttc cacgcgtatt gacctcacgc
    23821 tggctgactg ggcgcagcag cgacgtgatt acaccgccgc ccgcgctgca tatcagaatg
    23881 tcctgacgct ggagccaact aacgccgatg ccattctggg gctgacggaa gtggatattg
    23941 ctgccggtga cacagcggcg gcacgtagcc agctggcgaa actgcccgct accgataacg
    24001 cctcgctgaa cacacagcgg cgcgtggcgc tggcgcaggc gcagcttgga gataccgcag
    24061 cggcgcagca gacgtttaat aagttgatcc cgcaggcaaa atctcagcca ccgtcgatgg
    24121 aaagcgcgat ggtgctgcgc gacggtgcga agtttgaagc gcaggcgggc gatccaaagc
    24181 aggcgctgga aacctacaaa gacgccatgg tcgcatccgg tgtgactacg acgcgtccgc
    24241 aggataacga cacattcacc cgactgaccc gtaacgacga gaaagatgac tggctgaaac
    24301 gcggcgtgcg cagcgatgcg gcgggcctct atcgccagca ggatcttaac gtcaccctcg
    24361 agcacgatta ctggggttcg agcggcaccg gtggttactc cgatctgaaa gcgcacacta
    24421 ccatgttgca ggtggatgcg ccgtattctg acgggcggat gttctttcgc agtgatttcg
    24481 tcaatatgaa cgtcggcagt ttctccacta atgccgatgg caaatgggat gacaactggg
    24541 gcacctgtac attacaggac tgtagtggca accgcagcca gtcggactcc ggtgccagcg
    24601 tggaggtcgg ctggcgaaat gacgtctgga gctgggatat cggtaccacg ccgatgggct
    24661 tcaacgtggt ggatgtggtc ggcggcatca gttacagcga tgatatcggg ccgctgggtt
    24721 acaccgttaa cgcccaccgt cggcccatct ccagttcttt gctggccttt ggtgggcaaa
    24781 aagactcccc gagcaatacc gggaaaaaat ggggtggcgt acgtgccgac ggtgtggggc
    24841 taagtctgag ctacgataaa ggtgaagcaa acggcgtctg ggcatcgctt agtggcgacc
    24901 agttaaccgg taaaaatgtc gaagataact ggcgcgtgcg ctggatgacg ggctattact
    24961 ataaggtcat taaccagaac aatcgccgcg tcacaatcgg cctgaacaac atgatctggc
    25021 attacgacaa agatctgagt ggctactcac tcggtcaggg cggttactac agtccgcagg
    25081 aatacctgtc gtttgctata ccggtgatgt ggcgggagcg cacggaaaac tggtcgtggg
    25141 agctgggggc gtctggctcg tggtcgcatt cacgcaccaa aaccatgccg cgttatccgc
    25201 tgatgaatct gatcccgacc gactggcagg aagaagctgc gcggcaatcc aacgatggcg
    25261 gcagcagtca gggcttcggc tacacggcgc gggcattact tgaacgacgt gttacttcca
    25321 actggtttgt tggcacggca attgatatcc agcaggcgaa agattacgca cccagccatt
    25381 tcctgctcta cgtacgttat tccgccgccg gatggcaggg tgacatggat ttaccgccgc
    25441 agccgctgat accttacgcc gactggtaag ttttcagata gcgcctctct taatgccgct
    25501 gcgatcgggt atactcgggc ggcaatctgg gatttccggg gggagacaat ttgcgcgtaa
    25561 gtcgctcgtt aacaatcaag cagatggcaa tggtggcagc cgttgtcctg gtgttcgttt
    25621 ttattttttg caccgttttg ctgttccatc tggtccagca gaatcgctat aacacggcta
    25681 cgcaactgga aagcattgct cgctctgtac gcgaaccctt atcttcagct attttgaaag
    25741 gcgatattcc cgaagcggaa gctattcttg ccagcattaa accggcaggc gtggtcagcc
    25801 gtgccgatgt agtgctgcct aaccagttcc aggcgctgcg taaaagtttt attccagagc
    25861 gcccggtgcc ggtaatggtt actcgcctgt ttgagctacc ggttcaaatc tcgctgggcg
    25921 tttactcgct cgaacgtccg gcaaacccgc agccaattgc ctatctggta ctacaggcgg
    25981 attccttccg tatgtataag ttcgtgatga gcaccctctc aacgttagtg accatttact
    26041 tacttttgtc gcttatcctg accgtcgcca tcagctggtg cattaaccgc ctgattttgc
    26101 atccgttacg caatattgct cgcgaactta acgccatccc agcccaggag cttgttggtc
    26161 accaactggc attaccgcgt ctgcatcagg acgatgaaat cggtatgttg gtgcgcagtt
    26221 acaacctcaa ccagcaattg ctgcagcgcc attatgaaga acagaacgaa aatgcgatgc
    26281 gcttcccggt gtcggatttg ccgaacaaag ccttgctgat ggagatgctg gagcaggttg
    26341 tcgcgcgtaa acaaaccacc gcgctgatga tcatcacctg tgaaaccctg cgtgatactg
    26401 cgggcgtgct gaaagaggcg caacgagaaa ttctgctgct gacgctggtg gaaaaactca
    26461 aatcggtact gtcgccacgt atgatcctcg cgcagattag cggttatgac tttgctgtca
    26521 ttgccaacgg tgtacaggaa ccgtggcacg caatcacctt aggtcagcaa gtgctcacta
    26581 tcatgagcga gcgcctgccg attgaacgta ttcaactccg tccgcactgt agcattggcg
    26641 tggcgatgtt ctacggcgat ctcaccgccg aacagcttta cagtcgcgct atttctgcgg
    26701 catttaccgc tcgccataaa ggcaagaatc agattcagtt ctttgatccg cagcagatgg
    26761 aagccgccca gaagcggttg acggaagaga gcgatatcct taatgcactg gaaaatcatc
    26821 agtttgctat ttggttacag ccacaggtcg agatgaccag cggtaaactg gtcagtgcgg
    26881 aagtgttact gcgtatccag caaccggatg gcagttggga cctgccggat ggcttaatcg
    26941 atcgcattga gtgctgtggg ctgatggtta ccgtcggtca ctgggtgctg gaagagtcct
    27001 gtcgattgct tgcagcctgg caagagcgcg gcattatgct gcccttgtcg gtaaacctct
    27061 ctgcgctgca actgatgcac ccaaatatgg tggcggatat gctggaactg ttaacccgct
    27121 atcgcattca accgggaaca ctgattctgg aagtgacaga aagccgacgt attgacgacc
    27181 ctcatgctgc ggtggcaatc ctccgtccgc tgcgcaatgc cggagttcgg gtggcgctgg
    27241 atgatttcgg catgggctac gcagggctgc gtcagctgca gcatatgaaa tcgttgccaa
    27301 tcgacgtact gaaaatcgac aaaatgtttg ttgaaggctt gccggaagat agcagcatga
    27361 ttgctgcaat tatcatgctg gcgcagagcc tgaacttaca aatgattgcc gaaggcgtgg
    27421 agactgaagc acaacgcgac tggctggcaa aagcgggcgt tggtattgcc cagggcttcc
    27481 tttttgctcg cccactccct attgaaatct tcgaagagag ttacctggaa gaaaagtagc
    27541 taccccaaac tgattacaaa actttaaaaa gtgctggttt gtgcgagcca gctcaaactt
    27601 tttaaccttt ttgtttcaat tatgatccag gtacatttct gtgatgttgt ctgggtgtta
    27661 ttttaaggcc gcaggtacct cataacctta caagacctgt agttttacta aaggacaccc
    27721 tatgaaaacc tctctgttta aaagccttta ctttcaggtc ctgacagcga tagccattgg
    27781 tattctcctt ggccatttct atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg
    27841 cttcgttaag ctcattaaga tgatcatcgc tcctgtcatc ttttgtaccg tcgtaacggg
    27901 cattgcgggc atggaaagca tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta
    27961 ctttgaaatt gtcagtacca tcgcgctgat tattggtctt atcatcgtta acgtcgtgca
    28021 gcctggtgcc ggaatgaacg tcgatccggc aacgcttgat gcgaaagcgg tagcggttta
    28081 cgccgatcag gcgaaagacc agggcattgt cgccttcatt atggatgtca tcccggtgag
    28141 cgtcattggc gcatttgcca gcggtaacat tctgcaggtg ctgctgtttg ccgtactgtt
    28201 tggttttgcg ctccaccgtc tgggcagcaa aggccaactg atttttaacg tgatcgaaag
    28261 tttctcgcag gtcatcttcg gcatcatcaa tatgatcatg cgtctggcac ctattggtgc
    28321 gttcggggca atggcgttta ccatcggtaa atacggcgtc ggcacactgg tgcaactggg
    28381 gcagctgatt atctgtttct atattacctg tatcctgttt gtggtgctgg tattgggttc
    28441 aatcgctaaa gcgactggtt tcagtatctt caaatttatc cgctacatcc gtgaagaact
    28501 gctgattgta ctggggactt catcttccga gtcggcgctg ccgcgtatgc tcgacaagat
    28561 ggagaaactc ggctgccgca aatcggtggt ggggctggtc atcccgacag gctactcgtt
    28621 taaccttgat ggcacatcga tatacctgac aatggcggcg gtgtttatcg cccaggccac
    28681 taacagtcag atggatatcg tccaccaaat cacgctgtta atcgtgttgc tgctttcttc
    28741 taaaggggcg gcaggggtaa cgggtagtgg ctttatcgtg ctggcggcga cgctctctgc
    28801 ggtgggccat ttgccggtag cgggtctggc gctgatcctc ggtatcgacc gctttatgtc
    28861 agaagctcgt gcgctgacta acctggtcgg taacggcgta gcgaccattg tcgttgctaa
    28921 gtgggtgaaa gaactggacc acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga
    28981 tggcaaaacg cacgaattat cctcttaatc tcaccactta tgcccgcact cccttccccg
    29041 agtgcgggtg tttgcgcata attgacccct gtgcctgaac ttaccacctg ggtgagttca
    29101 ggtttatttc acttcttccg tgacattttc atgttcttgc ggtctaacac gaagtgtttt
    29161 taacgtcatt tagactgact gacaacgggc agttttttat taccaggata gttgatcagg
    29221 ggttcacatg cagggcacaa aaattcgact tttagcgggc ggtttgctga tgatggccac
    29281 tgctggctat gtgcaggcag atgcgctcca gcctgatcca gcatggcaac aggggacgct
    29341 ttccaacggt ttacagtggc aagtgctgac caccccccag cgtcccagcg atcgtgttga
    29401 aattcgcctg ctggttaata ccggttcgct cgccgaaagt acacaacaga gcggttacag
    29461 tcacgccatc cctcgtattg cgctaacgca aagcggtggc cttgacgcag cacaggcgcg
    29521 ttcattgtgg cagcagggga tcgaccctaa acgcccgatg ccgccggtaa ttgtctctta
    29581 tgacaccacg ctgtttaatc tgagtttgcc caataaccgt aacgatttgc tgaaagaagc
    29641 gctctcttat ctggcaaatg ccactggcaa attgaccatc acaccagaaa ccatcaacca
    29701 cgcgctgcaa agtcaggaca tggtggcaac ctggcctgcc gatactaaag agggctggtg
    29761 gcgctatcgt ctgaaagggt caaccttgtt aggtcacgat cctgccgatc cgctgaaaca
    29821 acccgttgaa gcggaaaaaa ttaaagattt ctatcagaaa tggtacaccc cggatgcaat
    29881 gacgctactg gtggtgggaa acgtggatgc gcgctcggtt gtcgaccaaa tcaacaaaac
    29941 gtttggcgaa ctgaaaggca aacgtgaaac gccagctccg gtgccgacgc tttctccgct
    30001 gcgtgcggaa gcggtgagta ttatgactga cgcggtgcgt caggaccggt tatctatcat
    30061 gtgggatacg ccgtggcagc cgattcgtga atcagccgca ctgctgcgct actggcgtgc
    30121 ggacctggcc cgcgaggcgc tgttctggca tgttcagcaa gcgttaagcg ccagtaacag
    30181 caaagacatc ggtcttggat ttgactgccg tgtgctgtat ctgcgtgcgc agtgtgccat
    30241 caacatcgaa tcaccaaacg acaagctgaa cagcaacctt aatctggtgg cgcgtgaact
    30301 ggcgaaggtt cgcgataaag gtctgccgga agaagagttc aatgcgttag tggcgcaaaa
    30361 gaaactggag ctgcagaaac tgtttgccgc ctatgcgcgg gctgataccg atattctgat
    30421 gggtcagcgg atgcgttcgt tgcaaaatca ggtcgtcgat atcgcgccgg agcagtatca
    30481 gaaactgcgg caggatttcc ttaacagcct gacggtggag atgttaaatc aggatctgcg
    30541 tcagcagttg tcgaatgata tggcgttaat actgctgcag ccgaaaggcg agccggaatt
    30601 taacatgaaa gcgttgcagg cggcctggga tcaaatcatg gccccatcga ctgctgctgc
    30661 cgccacctct gtcgccacgg atgacgtaca tcctgaagtg acggatattc cacctgcaca
    30721 gtaatgcctg atggtgatga gttagcacaa ccatcaggta tttatggatg agctggatag
    30781 aggggtaaca ctttatccag ccttttgcat atgctgcgtt tacgctggca tcgcctcacg
    30841 cgggataatc gcgccgcgat actgaataac ggtacttgcg gtcaggtgcc cgcgtttcgc
    30901 cgcgttttcc gcactgccgc ccgtcaaacg taccgccaga taaccggcgc tgaaagagtc
    30961 gccagctgcg gtggtatcga tcactttttc tttcggcagt ttcaccgccg gaacatccac
    31021 taacccttcg ccagcaatgg acaccaggca agaatccgcc ccgcgtttca ccaccacttc
    31081 tttcacgccc gcgttatggg tgcgcgcaat gacgtcttcc accggctgtt gaccccacag
    31141 cgcgtcttca tcgtccagcg tcaggaaggc gatatccgtg cattccagca tttgttggta
    31201 cacctgctgt gtctcttctt tgctggccca caggcgcgga cgatagttat tgtcgaaaat
    31261 cacttttccg ccgttggcgc ggcattcgcg cagcagggaa agcagctttt cgcggctggt
    31321 cgggcttaag atcgccaggc taatcccgct caggtagaga taatcgaaat tcgccagctc
    31381 ttcgcaaatc gccgtagact gctcactctc cagccagaat ttggcggcgg cttcgttccg
    31441 ccagtagtag aacgtacgct cgccggtgct gtcggtttca atgtagtaaa ggcccggcag
    31501 acggttttcc atccgttggg tcagggaaat atcaacgttc tcgccgtgcc aggcgtccag
    31561 catctgctgg ctaaaactgt ccgttcccag cgccgttacg taatgaacgg ttaatgccgc
    31621 aggatcgacc tgacgggcga tatagacgga agtgttcagg gtatcgccgc cgaaaccgcg
    31681 cttaacgtcc gcgcctttct cggaaagctc aatcatgcat tcgccaatca cggcaatctt
    31741 tttggacata gtcgtgaacc tgatctgtaa aaaattagcg ttagtttgcg ctgtggtggt
    31801 ttgctggtca acaatattaa aacagtgttc cattattttt ttgagccaga acgtattcct
    31861 gaaagattcc gttgtggagt gaggaaattt accccggcca ggcggtaaag ttctgccctt
    31921 acgcgccgat aatctttgtc gagtccgggc agcatcactt ttaaacacag gacatctttg
    31981 atgataaggc aggttatcca gcgaataagc aaccctgaag caagcatcga gagcttgcag
    32041 gaacggcgtt tttggttgca gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca
    32101 tgcgggcggt taatggccgt ggagctatta acggtggtca cgcatccctt gaacccttcg
    32161 caacgcctgc cgccggatcg ctattttact gaaatcaccg tcagccatcg gatggaggtt
    32221 gtgaaagagc agattgattt gctggcgcaa aaagccgact tctttataga gcacggcctg
    32281 ctggcatcgg tcaatattga tggccctacg ctcatcgccc tgcgtcagca accaaaaatc
    32341 ctgcgccaaa ttgagcgtct tccctggctg cgtttcgaac tggtggagca tatccgtctg
    32401 ccgaaagatt caacctttgc ctcgatgtgt gaatttggcc cgctgtggct gggtgatttt
    32461 ggtaccggga tggcaaattt ctctgcacta agtgaagtgc gttatgacta catcaaaatc
    32521 gcgcgagaac tgtttgtgat gctgcgtcag tcgccggaag gacgcacact cttttctcag
    32581 cttttacatc taatgaatcg ctattgtcgc ggggtgattg ttgagggcgt agaaacgccg
    32641 gaagagtggc gtgatgtaca gaactcgccc gcattcgccg cacaaggctg gtttctttca
    32701 cgcccggcac cgatagaaac gctgaatacg gcggttctgg cgctataagc tgcctcattt
    32761 tccgcctggc tggactatct ttaggactgg cacaggaaga atatgaggaa gcgaacgatg
    32821 agcaaggcag gcaaaataac tgctgcgatt tcaggggctt tcttgttgtt gattgtcgtg
    32881 gcgatcattt tgattgcaac ttttgactgg aatcgactca aaccgaccat caaccagaaa
    32941 gtctctgcgg agttgaatcg tccgttcgct atccgtggcg atctgggcgt ggtgtgggag
    33001 cggcaaaaac aagaaactgg ctggcgcagc tgggtgccgt ggcctcatgt acatgcagaa
    33061 gacatcattc ttggcaatcc accggatatt cccgaagtca cgatggtgca tttgccgcga
    33121 gtagaggcaa cgctggcacc gctggcgctg ctgaccaaaa cggtctggct gccgtggatc
    33181 aagctcgaaa agcccgacgc gcgcctgatt cgcctctccg aaaagaacaa taactggacg
    33241 tttaatctcg ccaacgatga taacaaagac gcgaatgcaa agccgtcggc atggtcgttt
    33301 cggctggata atattctttt cgatcaaggg cggatcgcca ttgatgacaa agtaagcaaa
    33361 gcggatctgg aaatttttgt tgatccgtta ggcaagccgc tgccgttcag cgaagttact
    33421 ggatcgaaag gtaaagcgga taaagaaaag gtgggcgatt acgtttttgg cctgaaggcg
    33481 cagggacgtt ataacggtga acagctcacg ggtacgggaa aaataggcgg tatgctggcg
    33541 ctgcgtggcg aagggacgcc gtttccggta caggctgatt tccgttcagg taatacccgt
    33601 gttgcttttg atggcgtcgt gaatgaccca atgaagatgg gcggtgtcga tttacggctt
    33661 aaattttctg gcgattcact gggtgatctc tatgaactga cgggcgttct gctgcccgat
    33721 accccgccgt ttgaaacgga tggtcggctg gtagcgaaaa tcgacactga aaaatcgtcg
    33781 gtctttgatt atcgcggttt taatgggcga attggcgata gcgatatcca cggttctctg
    33841 gtctacacca ccggaaagcc gcgaccaaaa ctggaaggtg atgtcgagtc gcggcaattg
    33901 cggctggcgg acctgggacc gttgattggc gttgattcgg ggaaaggggc agaaaagtcg
    33961 aaacggtctg aacagaagaa gggcgaaaaa agcgttcagc ctgcgggcaa agtgctgcct
    34021 tatgaccgct tcgaaaccga taaatgggac gttatggatg ccgatgttcg cttcaaaggc
    34081 cgacgtatcg agcatggcag tagcctgccg attagcgatc tttctactca tatcatcctc
    34141 aaaaatgctg acctgcgcct gcaaccgctg aaatttggca tggcgggcgg cagcattgcg
    34201 gcgaatatcc atctggaagg cgataaaaag ccgatgcagg ggcgggcaga tattcaggct
    34261 cgtcgactga aactgaaaga actgatgccc gatgtggaac tgatgcagaa gacgctgggg
    34321 gaaatgaacg gtgacgcgga actacgcggt agcggtaact cggtggcggc gcttttaggc
    34381 aacagtaacg gcaacctgaa actgttgatg aatgacgggc tggtgagccg caacctgatg
    34441 gagattgttg ggctgaatgt cggcaactac attgtcggtg cgatatttgg tgacgatgag
    34501 gtgcgggtga actgcgcggc ggcgaatctg aatattgcca acggcgtagc gcgcccgcag
    34561 atttttgctt tcgatactga gaacgcgttg attaatgtta ccggcacggc aagttttgct
    34621 tcggaacagc tggatttgac tattgatccg gagagtaaag ggattcggat tatcacactg
    34681 cgttcgccgc tgtatgtgcg tgggacgttt aaaaatccgc aggctggggt gaaagccgga
    34741 ccgctgattg cccgtggtgc cgttgcggcg gcactggcaa cgctggtaac accggcggcg
    34801 gcgttattgg cactgatctc accttccgaa ggggaggcta atcagtgtcg gacgatattg
    34861 tcgcagatga agaagtgatg aggttggatt gtgtttgctg gtgagttcgg gagcagcgtg
    34921 ttcccctcac cctaatcctc tccccaaagg ggcgaggaga ctgttttgtg cgtgttgaaa
    34981 caacgagtac cgcttttccg ttcactttct gggggagggc tgttatgttc gcgatataac
    35041 atctcgctcc gcttttctcc ctctcccttt cagggagagg gctggggtga gggtggctgc
    35101 ccacacgata cttacaacga ctgatgttgc gtctcatggg tcagcagcag ggcgattaac
    35161 gtcaagccag ccatcgccgc cagatataac cccaccgcac ctaacccgta gttagtctgc
    35221 aaccaggctg cgatatacgg cgcaacggaa gccccgagaa tcgacgatac gttgtaagag
    35281 aacgatgctc cggtgtaacg cacttctgtc gggaacagct ctggtaacag cgcgcccatc
    35341 ggaccgaagg tcagccccat cagacttaac cccagcagca ggaaggcaaa aaccagaatc
    35401 gggttgccag aaccgagcaa agggttaaag gcgaatagcg cgaacaggat gagcagcgtg
    35461 gtgatgatta ccatgctttt acggcgacca aaggcatcag ccagtaatcc agcgactggc
    35521 accatcacgc caaaaccaat aactgccatc atcaacatcc acaacacttc gttacgcggc
    35581 aggccaagcc caactggcgc ggcggcggta ctaaaggtca tggagtagac cgtcatgatg
    35641 taaaacagcg tataggttgc cagcataatg aacgtaccca gtacggttac gcgaacatgt
    35701 ttggtcagca gcgtacccag cgggatcttc acctgttttt tcgctttagc gactttctca
    35761 aacaccggcg actcatgcag cgacacgcga acatacaggc cgataatgac cagcaccgcc
    35821 gagaagataa atggcacacg ccagccccag ctcataaact gctcgtcggt cagtagccag
    35881 gaaagcagca agaaagtgcc gttggcaaag aagaagccaa tcggtgcgcc cagctgcggg
    35941 aaggagccgt acaatgcacg tttgcgcggt ggggcgtttt cagtcgccag cagcgccgcg
    36001 ccgccccatt caccacctaa gcccagaccc tgaccaaatc gagccagcgc cagcagcagc
    36061 ggggcgaaaa taccaatcgt ggcatagcct ggcagcagac caatcaccac ggtcgaaatc
    36121 cccatcgtta gcaacgaggc gaccagcgtc gctttacgcc caacgcgatc gccaaaatga
    36181 ccaaaaacgg cagagccaat ggggcgcgca acgaaggcga tggcgaaggt ggcgagcgac
    36241 tgtagcgttg ctgccgtagg atcgccctgc gggaagaaga tatgcggaaa cacaataacg
    36301 gccgcagtgg cgtaaatgta aaagtcgaag aactcaatgg ctgtgccaat gagagaggcg
    36361 acaaggactt tattacgcga gttgatcggc gtgtattctt gctcgtggtc gagtgttgtg
    36421 gctgttgctt gcataatctt ttcttatttt ttggctaacg aatagccata ttacgcacag
    36481 caaaagcgat atttcaatgt gtaacaaatc gcggaaatgg tcaaaaacca ggcaaaaagg
    36541 ggataatgtt taggccagtg atttttcttc tatgaaatgc ttcacataat tttataaata
    36601 gtggataaaa ctggtttcag gttaaataaa agttaccaac tggatttata tgctggggtt
    36661 atgagttaga atgaaattgt ttgctcccaa atagatggct gaagaggctc tggggacgag
    36721 tttcaagagg ttatgtgcgc attatagccg gatgcgatgc tggcgcatct tatccggcct
    36781 acggggagcc aggccgaata aaatcgtatg ccggataagg cgtttacgcc gcatccggcc
    36841 tattttaagg ctgcgttttc cctagcattc gcgggtcgtc tttatattcg gcggtggcaa
    36901 tccacgccgc gcaaaacagc gtcaaacggg cgaagaagta gaaaaatgcc atcagcccca
    36961 gcacggaacc aaatgctgcg ccagaggggg atttcatcag cgatggcagg gtgtaggtca
    37021 tcacgatttt aatcacttca aaaccaatag cagccagaaa tgttccgcga atcagggctt
    37081 ttttgcgcgg acggtggcgc ggcaaccgcc agaagatcca gaagaaaagc agatagttgg
    37141 cgaagatgga aatcgccagg ccaatcaatc gccacgtcgg tttcagccac tcaatgctgt
    37201 tcaggtgcag ggcgctaata atcatttgct gcgccgaacc ggcaaccgag gtgatcgaaa
    37261 gcgtcacaat cagcgcaatc aacaaaccaa tcagtgaaat aaaatcacgc agatatttta
    37321 cccagaactt ctcctgatct tgcggcgagc gttcccagac atcacgcgac tgggcacgaa
    37381 tcgcttcacg caggttaccc atccagttga tgccggaata aagtgccacc gccaggccga
    37441 caagccctac agtcgtacgc tgctgaacgg cggtgttgat ggtgtttttc aacgtggcgg
    37501 ctagtgtcgg atcgctgatg ttttgcagaa ttttgtcgaa gatatcctgt agcaacatcg
    37561 gatgggaggc cagcacaaag ccccctgcgg caaacgacac catcaaaatc gggatcatcg
    37621 acaaaaatga gaaataggtg atagccgcac caaactggtt acccagccga tcgttaaagc
    37681 gttctgtcgc gcgaatcagg tgcgcaataa ccggttggcg ttggactttt tcggcagtgg
    37741 tggtgacggt ttccagcgcc tggctaactt tgccgccttt ctcgctatta tccaactgct
    37801 taatcggctc gagctccaga tcctgggtgg gacgtttgat ctcgttttcc tgcgtcatcc
    37861 gctcttttgt ccttctgtcg tggtcattct taaaaagtat agtcggtcag tccaggtgct
    37921 ctttcattac gccagccagc cactgcataa acaggtttac acgccgggaa agctcccgac
    37981 gctgcgggta aaccagcgaa agggagagcg gctcggcacg gtagccaggt aatacttcaa
    38041 taagccgccc ggcacgcagg gcttcgcgca cggcaatgcg cgggatctga ataatcccca
    38101 gaccggcaag accggcggcg agatagtttt cgctactatt taccgtcaac atgccgccag
    38161 acttaaacca ctggacgcca ttaacgctgg caacctcaaa acctaacgga tgtacaccca
    38221 ggtgcggtgt ataacgcact attgcgtgtg aagtcagatc atcggggctt tgcggataac
    38281 caaagcgcgt cagatagtgc ggactggcac agttgaccat ggtcagtttg ccgaggggac
    38341 gggcgataac gccatcttcc ggtaacgcgc cagtgcgtat cacgcaatca aaaccatcat
    38401 gaagaatatc taccggacgg tcatggctac tcagttccag ctcaattccc ggatgcagat
    38461 agagaaattc cgacaggcgc ggcagtaaca ggtttttcgc gattcctggc gggatgtcga
    38521 tgcgtaattt accgctgata ctggtggcat cctgttgaaa cagaccgtcc agttcgttga
    38581 gattactcaa cacatctttt gcccgttgat aataggtcat gccttctggc gtgagtttga
    38641 cccgtcgcgt ggtgcgctgg agaagctggg tgccaagttg atgttccagt gcctgtatct
    38701 ggcgcgaaac acttcccttt ggcaaagcaa agaaatccgc tgcgcgggaa aaactttcca
    38761 gctccgcgac tttgatgaac aactgcattg cgtgaatttt atccatttct ttgaccgatt
    38821 gttgttcaca gcgaaacagt aacgcgcata tcgcattgtt tattgttttt tctaacatct
    38881 aatgccgggt ttcacaatgt tgtcatgacg aatgtggcga tttctcagca ggaaaagggc
    38941 ggaatagctt tatgagaaag gtgaatcccc ttaaacagag cgtatttttc agtgattccc
    39001 tttaaagtga atagaggacg tattggctat ttgtgcttat ttttgtctca ttgccatttg
    39061 ttattttggc ttgtagtcgc ggaatttcat ttttaattgc atgcgtacta accaccaata
    39121 taaggaaatc ctcaaaattg ttttaaatag tgccctcgac atgtaatttc aggcgtttag
    39181 ctgtaactat ttatatcggt aactattttc tacgcgtcat ctctgtagaa tggccaccgc
    39241 caaatggcga attcttattt tttctcgcgg gtaataccct tccactactt aagtcttttt
    39301 aacatattca ggtggagatt cagttatgca aatagtcatg tttgacaggc agtcaatatt
    39361 tattcatgga atgaaaatca gtttacagca gcgtattcca ggggtgagta ttcagggggc
    39421 cagtcaggca gacgagttat ggcaaaagct ggaaagttac cctgaagcct tagttatgct
    39481 cgatggcgat caggatggtg agttttgcta ctggttgctg caaaaaaccg tggtgcagtt
    39541 tcctgaggtt aaggtgttaa tcacggcgac ggattgcaat aaacggtggt tacaggaagt
    39601 tatccatttt aatgtgctgg ccattgtgcc tcgtgattca accgtcgaga catttgcgtt
    39661 ggcggtaaat agtgcggcga tggggatgat gtttctgcct ggagactggc ggacgacgcc
    39721 ggaaaaggac attaaagatc tcaaatcgtt aagcgcccgc cagcgggaga ttttaaccat
    39781 gttagcggcg ggcgagtcaa ataaggagat cggcagagcg ctgaatatca gtaccggaac
    39841 ggtaaaagcg catctggaat ccctgtatcg ccgtctggaa gtgaaaaatc gcacccaggc
    39901 ggcaatgatg ttaaatatct cctcctgaag aggataattt tacagccagc aggaagttag
    39961 cggctggctg taaaaatatt atggttcgcc gtacaaacca attaaacggc gtaccacacc
    40021 gttagtccag ccaaacccat cctgcaacgg atactcgcca ccgccgcctt cgcgtggaac
    40081 accatcggca atatggtatt tttcgatcag tttgtgctgt tccagataga actgattcac
    40141 cgtcttcagc cagcttcgcg cgatttcatc acccagaagg tcatcgccgt acattttaaa
    40201 tccctgaatc gccatccatt gtaacggtgc ccagccgttg ggtttatccc actgttcacc
    40261 ggtttcgtac tcgcttgcca gaatcccgcc aggtgtcagt aaccgactgc gcacggcatt
    40321 tgccagacga tcggcctgtt catggttcgc cataccgaca tagagtggca caatggcggc
    40381 agcggaaaac agcgccagtt gttcgcgtcg ccagtcgtaa tcgcggtaga tgccgttttc
    40441 atcatcccag aggtaacgct ttaccgcatc gcgacgggca ctggccttct ggcggaacag
    40501 tgcttctgtc tctttctcgc ctttcagcgc cgagatgttg gcgatggcgc tctccagttt
    40561 aaacaggaag gcattcagat cgatggggat gaactgggtg gtacgaatac tcgccagacg
    40621 accagtatca cgcagccaac gggaagagta atcccaaccg gaggccgccc ccgctcgtaa
    40681 atcgcggtac acctcgttgg gcgggcgacc agaatgtttc gcggtttcaa cgtcctcaag
    40741 ccaggattcg tcacgcggcg tgtcgcgatc gtcccagtaa cggttgagca gcgatccgtc
    40801 cggcatccgc acaacatggc gataggcctg attagggatc aacgattctg caccgtccat
    40861 ccagaaggca tattccattt tcaggtggtc gagatagcgg cgcgcaccgc gtacaccatc
    40921 ttcttcaaac aactccacca tcagcgcaaa aaccggtggt tgcgagcggc tcaaataata
    40981 ggtgcggttg ccgttgggga tgtgaccgta gttttcgatc atccaggcga agttatcggc
    41041 catgcatttc agcaaatctt cccgaccact ttccgccagc cccagcatgg tgaaatagga
    41101 atcccaatag taggtttcgc taaaacggcc gcccgggaca atatatgact gcggtagcgc
    41161 cagcagagaa gaccacggaa tgtgatcctg tggttcgcgg gttagcaccg gccacagctg
    41221 gtcgatatgc tctttcaggg aattttgcgg gtccgataca tactcgctgg agtagacctc
    41281 cggcagccag aagtgatttt caacaaactt gcgcaagtca aaatcacgat gacggcgcac
    41341 tttacggtag cggattaaaa tatccagcgg gtccattttg ggtgcgcagt cgggaaaggt
    41401 tttactgtcg gggaaaattt tcgccgactg aacatgctcg aacagttcga gatagcgatc
    41461 ggcaggcgtc agcgcatcag aggcgggcag cccttcaatc atctcgggtt ccggttctgc
    41521 ctcgatcatc tcatccagtt ttaattcata cgggtccagt tcatagcaga gatcgacttc
    41581 gatcatcagt tcgtctggat tagggttttg aattttctga ttgagcataa ggatccaacc
    41641 tccgaaattc gtcgctaatt atcggctttt ctttaagggt agtcattcgc tccgcctggt
    41701 gggcgacaaa gcgtgcggtg gatcacgttt attaacttgt caaaaaatat aacgcactaa
    41761 tatcaatgga atttataaaa atacggattt ggtgttgttt ttcattttca gaacattcaa
    41821 tcctgctctc gccgcaatca tgatatgcca ttcctgatct gggtaatagg cacaggctat
    41881 cttattgata gtttatattc atgtaattga ttgattccta tcaaaaagcg tatctccctg
    41941 ttttttatga tggctttgtc tcgcagagac gtttgcagca ttctggaaaa ttttctgggt
    42001 cacaataact caccaccgtg agggaagaat aatgaaaatg gtctcacgta ttaccgcgat
    42061 cggcctggct ggcgtcgcga tttgctattt agggttatct ggttatgtgt ggtaccacga
    42121 taataaacgc agtaaacagg ccgatgttca ggcatctgct gtcagtgaaa ataataaggt
    42181 tttaggcttt ctccgcgaaa aaggatgcga ctattgccac acgccttcgg cagaattacc
    42241 cgcctattat tatattcctg gcgcgaaaca gttgatggat tacgacatta agcttggata
    42301 taaatctttt aacctcgagg ccgtgcgtgc ggcactgctg gctgataaac ccgtttcgca
    42361 aagcgatctg aataagattg aatgggtgat gcagtatgaa actatgccac caacgcgtta
    42421 taccgcgcta cactgggcgg gtaaggtgag tgatgaagag cgggcggaaa tactggcctg
    42481 gattgcaaaa cagcgcgcgg aatattacgc cagcaatgat actgctccgg agcatcgcaa
    42541 tgaaccggtg cagcccatcc cgcaaaaact gcctaccgat gcgcaaaaag tggcgttggg
    42601 ctttgcgctg tatcacgatc cccgtttatc ggctgatagc accatttcat gcgctcattg
    42661 ccatgcgttg aatgcggggg gcgtcgatgg cagaaaaaca tcgattggtg ttggtggcgc
    42721 agttgggccg attaacgcgc cgacggtatt taactcagta tttaacgttg agcagttctg
    42781 ggatggtcgt gcggcaacat tgcaggatca ggcaggtgga ccgccgttga acccgattga
    42841 aatggcgtcg aaatcctggg acgaaattat tgctaagctg gaaaaagatc cgcagcttaa
    42901 agcgcagttc ctcgaagttt atccgcaagg tttcagtggc gaaaatatta ctgatgccat
    42961 tgctgaattt gagaaaacat taattacgcc ggattcccca tttgataaat ggttgcgtgg
    43021 agatgaaaat gcgctgacgg cgcaacagaa aaaaggctat caattattta aagataataa
    43081 atgtgcaact tgtcatggtg gtattattct cggcggacgt tcctttgaac cgttggggct
    43141 gaaaaaagac tttaactttg gggaaattac ggcggcggat attggtcgta tgaatgtgac
    43201 taaagaagag cgtgataaat tgcgccagaa agtacccggt ttacgtaacg ttgctttaac
    43261 ggcaccgtac ttccatcgcg gtgacgtgcc gacgctggac ggggcggtga aactgatgct
    43321 gcgctatcag gtaggcaaag agctgccgca ggaggatgtg gatgatatcg tagctttcct
    43381 gcacagtctg aacggggtgt acacgccgta tatgcaggat aaacaataac taatttgatc
    43441 gcccgaacag gaatgtttgg gcgattttta ttacgataat aaagtctgtt tttaatatta
    43501 tcatgttaaa tgtttatatt ataaaaagtc gtttttctgc ttaggatttt gttatttaaa
    43561 ttaagcctgt aatgccttgc ttccattgcg gataaatcct acttttttat tgccttcaaa
    43621 taaatttaag gagttcgaaa tggaccagaa gctgttaacg gatttccgct cagaactact
    43681 cgattcacgt tttggcgcaa aggccatttc tactatcgcg gagtcaaaac gatttccgct
    43741 gcacgaaatg cgcgatgatg tcgcatttca gattatcaat gatgaattat atcttgatgg
    43801 caacgctcgt cagaacttgg ccactttctg ccagacctgg gacgacgaaa acgtccataa
    43861 attgatggat ttgtcgatca a
//
LOCUS       DAJTSL010000009        42680 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTSL010000009 DAJTSL010000000
VERSION     DAJTSL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.009"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..451)
                     /locus_tag="OO433_000462"
     CDS             complement(<1..451)
                     /locus_tag="OO433_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR6049061.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGALGMVAKDDVVILISNRGE
                     TAEILATLPSLKKMGNYLISFIRSHHSSLAISCDLSVEIPVKSEADNL"
     gene            complement(420..1628)
                     /locus_tag="OO433_000463"
     CDS             complement(420..1628)
                     /locus_tag="OO433_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HCR6049062.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIQE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNLACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            complement(1628..3208)
                     /locus_tag="OO433_000464"
                     /pseudo
     CDS             complement(1628..3208)
                     /locus_tag="OO433_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(3240..3887)
                     /locus_tag="OO433_000465"
     CDS             complement(3240..3887)
                     /locus_tag="OO433_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6049063.1"
                     /translation="MIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNAI
                     SRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILNN
                     YENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEKI
                     KQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDLILEEKLYLSIRIKVLMD"
     gene            complement(3884..4063)
                     /locus_tag="OO433_000466"
     CDS             complement(3884..4063)
                     /locus_tag="OO433_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR6049064.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKNMVCRSLSILQT
                     GFFMSEMSKKINSTN"
     gene            complement(4323..5576)
                     /gene="amiC"
                     /locus_tag="OO433_000467"
     CDS             complement(4323..5576)
                     /gene="amiC"
                     /locus_tag="OO433_000467"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6049065.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            5808..7139
                     /gene="argA"
                     /locus_tag="OO433_000468"
     CDS             5808..7139
                     /gene="argA"
                     /locus_tag="OO433_000468"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6049066.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(7201..9027)
                     /gene="recD"
                     /locus_tag="OO433_000469"
     CDS             complement(7201..9027)
                     /gene="recD"
                     /locus_tag="OO433_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6049067.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(9027..12569)
                     /gene="recB"
                     /locus_tag="OO433_000470"
     CDS             complement(9027..12569)
                     /gene="recB"
                     /locus_tag="OO433_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6049068.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPKSVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNEKGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(12562..15450)
                     /gene="ptrA"
                     /locus_tag="OO433_000471"
     CDS             complement(12562..15450)
                     /gene="ptrA"
                     /locus_tag="OO433_000471"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6049069.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTQARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTTEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(15626..18994)
                     /gene="recC"
                     /locus_tag="OO433_000472"
     CDS             complement(15626..18994)
                     /gene="recC"
                     /locus_tag="OO433_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6049070.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRLWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDNI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(19007..19330)
                     /locus_tag="OO433_000473"
     CDS             complement(19007..19330)
                     /locus_tag="OO433_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6049071.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRLQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(19315..19722)
                     /locus_tag="OO433_000474"
     CDS             complement(19315..19722)
                     /locus_tag="OO433_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6049072.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPSGWSDFCPLKERALCQLP"
     gene            complement(19719..20282)
                     /locus_tag="OO433_000475"
     CDS             complement(19719..20282)
                     /locus_tag="OO433_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6049073.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(20273..20743)
                     /locus_tag="OO433_000476"
     CDS             complement(20273..20743)
                     /locus_tag="OO433_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6049074.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(20928..21722)
                     /gene="thyA"
                     /locus_tag="OO433_000477"
     CDS             complement(20928..21722)
                     /gene="thyA"
                     /locus_tag="OO433_000477"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6049075.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYAPHPGIKAPVAI"
     gene            complement(21729..22604)
                     /gene="lgt"
                     /locus_tag="OO433_000478"
     CDS             complement(21729..22604)
                     /gene="lgt"
                     /locus_tag="OO433_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6049076.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(22755..25001)
                     /gene="ptsP"
                     /locus_tag="OO433_000479"
     CDS             complement(22755..25001)
                     /gene="ptsP"
                     /locus_tag="OO433_000479"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6049077.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGLPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPILLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVVAFMERRGM
                     GGLIRGGL"
     gene            complement(25014..25544)
                     /gene="rppH"
                     /locus_tag="OO433_000480"
     CDS             complement(25014..25544)
                     /gene="rppH"
                     /locus_tag="OO433_000480"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6049078.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            26229..26918
                     /gene="mutH"
                     /locus_tag="OO433_000481"
     CDS             26229..26918
                     /gene="mutH"
                     /locus_tag="OO433_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6049079.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWTPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            26987..27700
                     /locus_tag="OO433_000482"
     CDS             26987..27700
                     /locus_tag="OO433_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6049080.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            27838..28056
                     /gene="ygdR"
                     /locus_tag="OO433_000483"
     CDS             27838..28056
                     /gene="ygdR"
                     /locus_tag="OO433_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6049081.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            28164..29204
                     /locus_tag="OO433_000484"
     CDS             28164..29204
                     /locus_tag="OO433_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6049082.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLNLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGGMRYLHLADKHDLPSIVTIQTPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKTAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(29236..30429)
                     /gene="lplT"
                     /locus_tag="OO433_000485"
     CDS             complement(29236..30429)
                     /gene="lplT"
                     /locus_tag="OO433_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6049083.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(30422..32581)
                     /gene="aas"
                     /locus_tag="OO433_000486"
     CDS             complement(30422..32581)
                     /gene="aas"
                     /locus_tag="OO433_000486"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6049084.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDASRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTLDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            32970..34001
                     /gene="galR"
                     /locus_tag="OO433_000487"
     CDS             32970..34001
                     /gene="galR"
                     /locus_tag="OO433_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6049085.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(34008..35270)
                     /gene="lysA"
                     /locus_tag="OO433_000488"
     CDS             complement(34008..35270)
                     /gene="lysA"
                     /locus_tag="OO433_000488"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6049086.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRSLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            35392..36327
                     /gene="lysR"
                     /locus_tag="OO433_000489"
     CDS             35392..36327
                     /gene="lysR"
                     /locus_tag="OO433_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6049087.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASICAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(36314..37006)
                     /gene="ygeA"
                     /locus_tag="OO433_000490"
     CDS             complement(36314..37006)
                     /gene="ygeA"
                     /locus_tag="OO433_000490"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6049088.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(37135..38550)
                     /gene="araE"
                     /locus_tag="OO433_000491"
                     /pseudo
     CDS             complement(37135..38550)
                     /gene="araE"
                     /locus_tag="OO433_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(38865..39626)
                     /gene="kduD"
                     /locus_tag="OO433_000492"
     CDS             complement(38865..39626)
                     /gene="kduD"
                     /locus_tag="OO433_000492"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6049089.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(39656..40492)
                     /gene="kduI"
                     /locus_tag="OO433_000493"
     CDS             complement(39656..40492)
                     /gene="kduI"
                     /locus_tag="OO433_000493"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6049090.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLHNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(40779..41960)
                     /locus_tag="OO433_000494"
     CDS             complement(40779..41960)
                     /locus_tag="OO433_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6049091.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PCTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            42215..42613
                     /locus_tag="OO433_000495"
                     /pseudo
     CDS             42215..42613
                     /locus_tag="OO433_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
BASE COUNT        10858 a        11411 c        10122 g        10289 t
ORIGIN      
        1 ctaaattatc cgcctcactt ttgacgggga tttcaacaga taaatcacaa cttatcgcaa
       61 gcgatgagtg atgactgcgg ataaaagaga tcaaataatt acccattttt ttcaaactag
      121 gcagtgtcgc caggatttcc gccgtctcac ctctgtttga aataagaata acaacatcgt
      181 ctttcgccac catcccaagg gcgccgtgta ccgcttccgt accgtgaaca aaaaacgagg
      241 gagttccggt gctggcaaat gttgcagcaa gttttctggc aataatgcca gatttcccaa
      301 caccaataaa aacaactttt ccctggcaag attgtaatat tttaattaaa ttcgcgactt
      361 ccgaaagggg ggaatcaata acctcttcta ctgctttatg ttcgttacgc ataaaacgtt
      421 taataagatg gataagatcc gtattgttca ttttcttttt cctgattaat atttttaatt
      481 acactcgcaa ggcgcgcaag ccccatatat aatttttctc gactgcaggc cagattaaga
      541 cgcagatagc cctctccttc agtaccataa acatgccccg ccataatacc tactctccct
      601 tcgttaatta atcgacggta aaatttatca aagggataac caagggccga gcaatcaacc
      661 cacgaaaaat agcagccctc agctggccat acgatcaatt ccggaatatt ttcatttaaa
      721 tactgcaagg taaattgaat gttagaggca atatgtacct ttaatgactt taaccattct
      781 tcacattcat tataggcaac aatggtcgca attacattca gtactgatgg ggagtttacg
      841 aaatcgcgat acctcgatat cgttctgaac ttatgattaa aattatcgtc agttgaaatc
      901 atatatgatc cggttagcgc aggaacatta aatgttttcg aaatcgaatt aaacaccacc
      961 actttgtcat cttttattgt tagcgctgac gtgaaactat gattaaaaac aaaatcacta
     1021 tgtatttcat ctgaacataa ccaaatatta tgccttgcac agcatgctgc gattctgtga
     1081 agatcgtctt ccgaccagac cgttcctgtc gggttatgag gattacataa taacaccatc
     1141 ttcgcgcttt taattttaag ctcaaagtct tgccagttga taacgtagcc tctcccattt
     1201 tctttcatca aagaggttgt tagcatgttt cgaccattat gcttaatcac atcaaaaaat
     1261 gcgttataaa caggcgtgaa aatgagaata ttatctccat tcaatgtcaa tatttctatt
     1321 gccttagata ttgaatacat cacgcttggg ctataggtga tccattctgg ttgaatctct
     1381 gttttatatt gttgatgata ccaattgact atcgaattca gatagtcatc atgctgccaa
     1441 cgactgtacc ctaaaatcgg gtgttccaga cgttttttta atgttctgat gatagcttcc
     1501 tgtatcggaa gatccatatc tgaaatagag aatggtaata catcttttac accaaatctg
     1561 tcttcacaaa agtcccattg tgtgcaatat gttcctttac gagaaattga ggtattaaaa
     1621 atatccatca acatatcccc atgtaattat ccatatcccg acgtagggcg atgacttttg
     1681 taccgataat gacttgtaag gtattttgat caagtttcac aaccgcaatt cctccggccc
     1741 tttttattgc agcctcattt atcaatccca tatccgcgat agttaaacgc aaacgagtta
     1801 cacaattatc cagtgattta atattttctt taccacccag agccttaacc atttgctccg
     1861 ctgcctgcaa ctctgattta tcatgaactg ggatgttagc tattgcagtt tcttcgtgag
     1921 cgttcagcgt gacaggactg gactcttcac ggcctacagt catcaggtta aatttgagaa
     1981 tggtccaacg gaacacaaag taataaatga agaaataagc cacttcaaca cataccgcta
     2041 atggccatcc cgttcttgtg ccacgtaaga caccaaagct aataaattga atcagatcgc
     2101 cagtaaagcc catcttcacg cccatatatg gcagaaccat gttagccaat ccattcataa
     2161 aggcatggaa gacaaacaac agcgggctga caaataagaa cagaaattca atcggctcgc
     2221 tgacgccgcc aataattacc gtgagcacac cggaaatcag caatgctttg atagcccttc
     2281 tgttttgagc atgagcagtg cgatacatcg ccagcgcggc tccagccaga ccatactgaa
     2341 tcatcagttt gccctgctcc attttccccg ccaaatccag cggaatgacc gcattctctt
     2401 tgacgtatgc cataaacatg ttcagggtgc cgtaatactc ttcaccgcca atcactgccg
     2461 atccgccgat tggggtaaag cggaaaactg atgtcaccaa gtgatttaag ccaaaaggta
     2521 tcgtcacgcg ttcggcaaca gcatagaaga aataaccaac aggaccggaa gttgaaatcc
     2581 agtgaccaat gagcatgaaa agattgaaaa atggcggcca gataaaagga atgatcagac
     2641 caaatccact catcacaatc agtgtaatga taggcacaag gcgtgggcca ctataaaacc
     2701 ctaattattc aggaatacgt aagttaacga tctttttata catgctggcg actaataacc
     2761 ctgccacaat cccacccaac acgctggtat tgtaggactg gatcccgagt atgatggttt
     2821 gcccatgtgt cgacatttgg tcagcagcta ccaataagtc gtgctgttta agataaaagt
     2881 tcgttccaag atgcatcgcc ataaagccaa ttaagccaga aaaagcgcca taggctttat
     2941 catctttctc ttttaataat cccaaaggta tagctatcgc aaacaataca ggtaaattaa
     3001 cgaaggcaaa caaaccaagg ctaacaataa aatcaagtat ggttttaatt aacggaatag
     3061 ccaacactgg aatgaatttt gccatatcat cgctggctaa accacttccc agccctaaca
     3121 tcataccaca tacacttaac agagcgatgg gatacataaa tgccttcccc aggctctgaa
     3181 aaaaactcca ggatttcttt tgtttcatac tggttatctc atgaaaatgt tatgtataat
     3241 tagtccatta atactttgat gcgaatagaa agatatagtt tctcttccag aatcaaatca
     3301 tatttaaaag ttgcgtacag atatctttca atttcatgaa tggcacgttg taactgaaaa
     3361 tctttttcaa gaaactgctc atagatatca ccaatatttt tctgttttat tttttcatta
     3421 cataacacac gactagaaaa atatttaagg tgcatcataa atctggagta gttaaatgaa
     3481 cttctgtcca cccttctgtt ataaacaact tcaataagct ccgtgatttt ttgagacagc
     3541 tcaataatcc ttactgattc ataatcaact gaattttcat aattatttaa aatatgcaat
     3601 gctataaaac caatctcatc atccggtagt tgaacgctaa attgctcatt aatgattgtt
     3661 aatgcatcac gacttattgc atactctgct ttgtacaacg ctttgatttc ctcaaggaaa
     3721 acattattaa tttgtatgcc tgagacgact cgggatattg cattcgaaat gtgatcagca
     3781 agaatcagta gtagcgatgg attgagtttt ttttcgagat tactctccgc ataatgcact
     3841 atcttttcgg caacaaaaat atactcaata cccacatgct caatcaattt gtagagttta
     3901 tttttttgct catttctgac ataaaaaatc cggtttgcag gatgctcagg gaccgacata
     3961 ccatattttt attgtaacca acgccaggcc cagaaatgat aacctcctgt ccgttcaccg
     4021 atgcctgtac acaattattg ttcatgactt tttcgatgat cattccatgg ctccccaaaa
     4081 aaaaggcaag gcatgtcaga agtaataatt acttcaaaca ttgccttgcc tgatttaaca
     4141 gttgcaagat aattaactaa tgcgtatacc ttacacggaa ttgtgcttaa taaaagagat
     4201 gacagactta ttttttaagc cgatattcaa atattcgaca gcagtcacaa aaccatcgtt
     4261 caacatttat gtaaataata tttattcaat gaaacataaa ttcattgaat aaattaaaaa
     4321 catcatcccc ttctcgccag cgtcgcccca tcggcaaaat acgctttaat ccccgcaaga
     4381 atagactccg caacttcctg ctggaaagtc gccgttttca gtttacgctc ttcctcaacg
     4441 ttactaataa acgccgtttc gaccagaatg gagggaatat ctggtgcctt tagtacggca
     4501 aacccggcct gttcaacttg atttttatgc agcttgttga ttttaccgag cttattcagt
     4561 accgctttac caaacttcag gctgtcggca attgtcagcg actgcaccat atcgaacatg
     4621 gtgtggtcga catagcggtc accgcttttg ctcacgccac caatcaagtc cgaggcgttc
     4681 tgggtttgtg ccagatattt tgccgcagta ctggttgcac ctttggttga gagcgcaaac
     4741 acagaggaac cgctcggctg acgactggta aaggcgtcgg catggataga gacaaacagg
     4801 tcagcacgct gtttctgggc ttttgctacg cgcacttgca acggaatgaa gatgtcttca
     4861 ttgcgcgtca tgtacacctt catattgccc tctttctcga tcagagagcg cagacggcga
     4921 gctatttgca ataccacgtc tttttcgcgc gttttgtatt tccccaccgc accggagtct
     4981 tcgccgccgt ggccagggtc aagcataata acaataggac gatcgcgccc tgctttaccc
     5041 ggttgtggac cactttgtgc tggcggcacc tgcttttcga ggtcgccttt gttgtaatcc
     5101 tccagcagcg ccagcagcgg gtcctgcata tcctgtgcat tggccggata gaggtccatc
     5161 accagacgct ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg ttttacgttt
     5221 tgctttaatt caaaaaccat ccgtacggtc tgcgggtcaa attgcccgac gcgcgccgac
     5281 ttgatgaacg ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac cgagttcagg
     5341 tttacatctt cgatatccac caccacgcgt tcagggttac tcaacgcgaa ctgcttatat
     5401 ttcagctgac gatttgattc taccgtcaca cgggtgtagc tggacgcagg ccagacgcgc
     5461 accgccacga cctggctgac cgcagccaga ctgacctgac ttacgctcaa tagccacatg
     5521 gcacccgcgc cttgcagtaa acgacgacgg ctgattgcag tgttggatcc tgacatgcct
     5581 ctcccgagca aaagaaatct aagctgtgta acaagtaaac gactaatttg accggaaact
     5641 ttagcgaaag acgcataatc tgtcatctaa taaacggtaa acattctttt tatattcacg
     5701 gcattactga taaaaaagtc gctctcgcat aaaatttaca cttgcaccct gcgaaaaaac
     5761 agaataaaaa tacactaatt tagaataatc atgcaaaggg gtgtgccgtg gtaaaggaac
     5821 gtaaaaccga gctggtcgag ggattccgcc attcggttcc ctatatcaat acccaccggg
     5881 gaaaaacgtt tgtcatcatg ctcggcggtg aagccattga gcatgagaat ttctccagta
     5941 tcgttaatga tatcgggttg ttgcacagcc tcggcatccg tctggtggtg gtctatggcg
     6001 cacgtccgca gatcgacgca aatctggccg cgcatcacca cgaaccgctg tatcacaaga
     6061 atatacgtgt gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg ggaacattgc
     6121 aactggacat cactgctcgc ctgtcgatga gtctcaataa cacgccgcta cagggcgcgc
     6181 atatcaacgt cgtcagtggc aattttatta ttgcccagcc gctgggcgtc gatgacggcg
     6241 tggattactg ccatagcggg cgtatccggc ggattgatga agacgcgatc catcgtcaac
     6301 tggacagcgg tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact ggcgagagct
     6361 ttaatctgac ctcggaagag attgccactc aactggccat caaactgaaa gctgagaaga
     6421 tgattggttt ttgctcttca cagggcgtca ctaatgacga cggtgatatt gtctcagaac
     6481 ttttccctaa cgaagcgcaa gcgcgggtag aagcccagga agagaaaggc gattacaact
     6541 ccggtacggt gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc gtgcgtcgct
     6601 gtcatttaat cagttatcag gaagatggcg cgctgttgca agagttgttc tcacgcgacg
     6661 gtatcggtac gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca acaatcaacg
     6721 atattggcgg tattctggag ttgattcgcc cgctggagca gcaaggtatt ctggtgcgcc
     6781 gttctcgcga gcagctggag atggaaatcg acaaattcac cattattcag cgcgataaca
     6841 cgactattgc ctgcgccgcg ctctatccgt tcccggaaga gaagattggg gaaatggcct
     6901 gtgtggcagt tcacccggat taccgcagtt catcacgggg cgaggttctg ctggaacgca
     6961 ttgccgctca ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc acgcgcagta
     7021 ttcactggtt ccaggaacgt ggatttaccc cggtggatat tgatttactg cccgagagca
     7081 aaaagcagtt gtacaactac cagcgtaaat ccaaagtgtt gatggcggat ttagggtaag
     7141 cgatgaaaat tgtcggatgc gacatgcgta acactcgtgc gtcgcatccg gcaattacgt
     7201 ttattcccgc gaactaaaca acgccgccag accactgcgc cgctcagtac gagtggcgat
     7261 tgccgcactt aatatacgct catcggcata cagcgacaga cggcgacgcg cgcgggtcac
     7321 cgcggtgtat accagctctc gcgttactac cggcgtgcgt tggctcggta aaatcaacgc
     7381 cgcatggtcg aactccgatc cctgcgattt atgtaccgtc atcgcccacg tagtttcgtg
     7441 ctctggcaag cgactcggtt gcacagactt aatattgccg tccggcatcg caaaccagac
     7501 gcgcgtcccc tgcccgcgat ccagcgcaat accgatatcg ccattaaaca acccaagcgc
     7561 gctgtcatta cgggcaatca tcaccggtcg accttcgtac caacgagagt gcggatgacg
     7621 atgaattttg cgcttctgtt gcataaactg ctcaattcgc tcattcagtc cagccacgcc
     7681 aaacggccct tcccgcaggg cgcacaaaag ttggtactca ttgaacgcct gaatgattaa
     7741 atccggctcg gcacgcgctt gcagcagatc cagataacgt ccataacccg caagagcttc
     7801 ctcaagcatc gcaatataat cttcgccgct ctgtaaaagt cgtttttcga tatcagtaaa
     7861 atcctgctga aaaacggttt tcactgccgt tttatcacca cggttgatcg ccgcagctaa
     7921 ctgaccaatg ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc agagactgtc
     7981 gcgcaaagat gccgcttctg tgccggttcc tgccggaacg tgagtccccg tcaggcggct
     8041 tagctgcctg gcacgctcgg cggtaaagcc cgcgttggca taagcgcaga tatcgcccag
     8101 cacagcccca gcctcaaccg aggccagttg atcacgatcg ccgagaaaga tcactcgcgc
     8161 atgatcgggc aaggcgtcga tcagtctcga catcataggc agatcgatca ttgacgcttc
     8221 atctaccacc agcacatcaa gatgcagcgg gttaccggca tgatgacgta aacgctggct
     8281 acccggctgc gcgcccagca atcggtgcaa agtgctggca tcttccggaa tgcgtttctt
     8341 ttgttcatcg gtcagcggta actgtcgcaa agccttgccg agagattcgg ttaagcgcgc
     8401 ggcagcttta cccgttggtg cagccagacg gatacggcag cgttcgccgt cggccatttg
     8461 aattaacgct gccagcaact tcgccacggt ggtcgtttta ccggtgcctg ggccgccgga
     8521 aatcaccgag atccgccgcg tcagcgccac tgccgctgca actttttgcc agttaatttc
     8581 atcgcttact ggaaaaagtt tgtccagggt ttgcgccagt agagcttcat caacctcaat
     8641 ggcatgattc acctcattga agaagcgcgc taccgtccgc tcgttacacc acatgcgatt
     8701 caaataaaga cgatcgccac agaggatcat cggcgtgggt tcatctcccc tgctgaccgc
     8761 ttgagaagcc agcaagcatt cttcccaatt ttgtagctca ccgatttcac tgacacaggt
     8821 cgccaacagc ggatgcgacg cctcgttatt ttccagtcgt gaaagcggca aacagacgtg
     8881 tccctctccg gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag gatgttcatc
     8941 tcccgccacg gtcagggcaa attgcacatc cagcgggcgt agctgtttgt gctccacagc
     9001 ttccagtaat tgcttttgca atttcattac gcctcctcca gggtcatacc ggcaaacatc
     9061 tcatccatca gggcaatcaa cccggcgttg ggtcgggttg tgtaaatccc ttgttgcgga
     9121 tgttctttat caacgccacg caggaacaga tagataacgc cgccaaagtg acgatcatag
     9181 tcgtaatcag caatgcgatg gcgcagataa cgatgcagcg ccagggtata aagctgatat
     9241 tgcagatcat agcggtgtgc ctgcatcgct gctgccatag cctgttgggt gtaagccgaa
     9301 ctgtcttcac ccagccagtt ggatttatag tcgagcaggt aataacgccc ttcgtggcgg
     9361 aatactaggt cgataaagcc ttttaacatg ccgcgtacct gcatgaactc cagcggcggg
     9421 cagccagcgg atagcggatc aaactggcga attagtgcat caagctggct agcgatgagc
     9481 ggttcgctaa tcggcaggta aaactccatc tccacctgtt tatcgcggtc ggaaagctga
     9541 ctcaggctaa cccccttttc attgagaggt gcctggagga cagccgtgat ccactcggtc
     9601 aacaccggtt cccactgcga ttcaaagccg ccgagttcca gtttttcctg tacccagttc
     9661 gggtcaaccg gctgggtaaa atcgaggtct tcaaacaaac tgtgcaagaa tgtccccggt
     9721 gacgcaccgc gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac gctgaccacg
     9781 cctgcggcat caacatccag ccgtggcatc agatcctgag cgataccgtg accacgctgc
     9841 tgcaagccgg agtagctggt gacacgccag ttatcgccgg gcaatcgttg tagtgtcctc
     9901 gcattcagtt ctgcagttaa agcatcatta acctgccagg gctggttatc accagtttgt
     9961 gccgtttgcc aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg aagccctgcc
    10021 gcatcttgcg gttccccttt ttgcagcaaa cgcccgagcg cactttggtg gacgtcggtg
    10081 tcaccttttt tatcgccacg acggcgcacc agcggtgcaa cgccgagact gcaatgccaa
    10141 accgaacgcg tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg ttcgacctcc
    10201 gcgaggtcga cgctttttgg cgcagcatta agatccaaaa ctgcctcaaa cgagtggcga
    10261 tcgtgataaa acgcctgatc ctggacgcgg aaattggtaa taaatggcaa ccagaccagc
    10321 ggatattcca gccctttcga tttatggatc gtgacaatct gcaccagatg tttatcactt
    10381 tcgaggcgca tttgttggct ggaggcatta ctgtctggct cgaggatatg ttgcgataac
    10441 cagcgtacca gcgcatgttc actttccagc tgcgttccgg cttcttgtag cagttcgctg
    10501 atatgcaaga tatcggtaag acgccgctca ccgcctgccg ttgccagcaa gttttctgca
    10561 atgttacgcg ccgacatcag cgcccgcagc atcggcataa cgccacgttt gcgccagatt
    10621 tgccgataac catcgaactc ttcaactacc gcatcccacg catgttcgtc attattcagc
    10681 gtttcgatat ccagcgcgtt cagccccatc attgacgttg ccagcgcact acgcagggtg
    10741 ttctcacgtt cgggcgtcat caccgcctgc aataaccaaa gcatttcctg cgcttccaga
    10801 gtttcaaaaa cactgtcgcg gttcgaaagg taaacggaag ggatttccag caacgttaag
    10861 gcatcgcgca cctgggcggc ctcctggcgg ctgcgcacca gcacactgat gtccgaagca
    10921 cgcaccggac gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg tccggcttgt
    10981 agccagtcgc ggatttgcgc agcacatacc tgcgccatgg tactttgata gtcgccaacg
    11041 ccgcagcttt cgccttccat cagccacatt ttcatcgcag gctgcgtttc acctttaaat
    11101 acaaaacgta acgcctgatt tttcccggct gatttcactg gaataaacgg tatttcgcga
    11161 aacatgaacg cgtcatcagt ctggctgaaa agcttgttca cactgttcac cattccaggt
    11221 gcggagcgcc agttggtgtc taaagtgtag tgggcgtgaa cttcactacg cgccttcata
    11281 taagtgaaga tatccgcacc ccggaatgca tatatggcct gcttcgggtc gccaatcagc
    11341 aacaatgcgg tttccggctg atggtgccag atacggcgaa aaattcggta ctgctggggg
    11401 tcggtatcct gaaattcatc gatcattgcc accgggaatc gcgtacggat cgccgctgcc
    11461 aacacctcac cgctttcgct acgcagcgcg gaatcgagcc gacttaacat gtcatcaaaa
    11521 cccaattcgc cacggcggcg tttttcacgc gctactgttt cgcggatctc agccaatgcg
    11581 cgggtgatca ccagatcgcg gatcgacaat ggttctgcaa gcagttgatc gatcgcctca
    11641 aacagtggat gtcgcggggt ttccccccca gctttcgtgc gatcttctaa gaaacgctgg
    11701 gagaattttt ccagcgactc cggcaactga taactgttgg tctcttcttc tgcccaggcg
    11761 ctgatcttgt cgatccattt agcctgattg ctacggttaa acttgcgtcg atcaatacca
    11821 gaagattcga tcagcgcatc cagttcaccc actgcgtcgc gccactgctg ttttaccgta
    11881 gcaatacgcg ccacaatttg cgcgtggcgg gaagccagcg tttcatcatc gggcggcggt
    11941 gctttgataa ccggcgcttc gccttgcaga taacgattaa tatcgcgcag caacgcctgc
    12001 ggccctttcc aggtttcaaa gacgacctgg gctatttcac gcggcagcgg gtagcagtgg
    12061 cgacgccaga aatcggcgca ggcctggtaa cgtagcagag actcatcttc aatcagctgc
    12121 tgctcaaaca gcatgccgga ttcaaaggca ttcaggttga gcatgcgctg gcaaaaaccg
    12181 tgaatagtaa agactgccgc ttcatccatc tgccgttcgg ctaacaacaa ccactgcgcg
    12241 gcttgcgctt tatcgtcgat ctcttccagc agacgttcgt acagtggatt gtcggtagtt
    12301 tcacgcagac aggcgatgcg caactcatgg atattgctac ggatacgacc acgcaattct
    12361 gccgtggcag cctcggtaaa ggtgaccacc agcagttctt caacggtcag cgggcgggga
    12421 aaggcggcgg aaccgcctag tccaagtaac aggcgcaaat agagcgccgc aatcgtaaag
    12481 gttttgcctg tgccggcaga ggcttcaatc aggcgctcac cctgtaaggg caagcgcaaa
    12541 ggatctagtg tctcggcgac atcactcatt cttttcactc atcaggggca ttgtttgctg
    12601 caacgcgctg acgttctccc acactttcca gccttcaggg tgtacatatt cggctttccc
    12661 gttctggctg ccggaaatct gcgacagaat agccatgcct tgcggctcga ccaccgcctg
    12721 atggaagaaa tcagcaagtt tttgcggcgt cagcagtttt atctgggcca cgattttatc
    12781 acgcgaatcg aagcgcatat tgccgcgatc gaaatcttta cttaacttcg atgcttcttc
    12841 gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc gcctgctgga tttgcgcaaa
    12901 ctcatctggc ttcatcgctc gcaattttgc ctctgtggtt gggaaaaacg ccttgtaacg
    12961 ctcccacaag aatgaaggct gtttatcatt gctttgcaaa aggaagccca tgccccactg
    13021 acgccccacg ctcattggaa acgcaaacac ggcatagccc aattgttctt cggtacgcaa
    13081 ctgattgtag aaccacggct gtacgatctg ccccaacaga gagctatagg ctgagctggt
    13141 gtattcatcg tagccagtcg gtacaaatac cgctgccagt gcggagtcgg tgctgttacc
    13201 ggctttttca aagatgacgg attgtttttt atcgaccact acatctttgt ttcgacacca
    13261 ctctgaacca tcagcgccca actgtttttg cacatcgcgt gcctgcgttg ttgcctgggc
    13321 ctcggtcatg ttgccgataa ccataaactc tggtcgagcc cctgatttta aggcgtcgcg
    13381 ataggccagc acctctttca acgtaatgga gggcaaaatt ttacgccgtt catctcgcga
    13441 gaagtacggc acttgcgaga gcatctgcgc gggcataatc gcctgctcaa acgctttacc
    13501 cttttctgcg gaatccatca tctggttata ccaagacttc gcctgctcaa gctgatcttc
    13561 cgtagcggta tagctaaagt acccctcgag caatgcctgg aacagctgcg gcagacgctg
    13621 ggtgtaacca ttagcattaa ccataaggcc gttgttagcg ttggtggaaa aacttatgcc
    13681 accaaccgac gcctggttgc ttaactgatc aagcgccagc cctgcgagat aatcattgag
    13741 cgcaaacatc acctgattgc gggcgctgtc catggctttc ggattacgca aaatcaggct
    13801 gacatcagct ttgggttcgc tggaaaaata acggcttggc gcatacacca cgcgcagatt
    13861 cgactcatca acaatcagct ctggatggtc atatttcttc tctgacttaa tcagcgagaa
    13921 atcgtcagga atataggggt taagctccgg taaggagagc gcaatattgg cagctttttg
    13981 ctgccagtca gcgaaagttt gttcgctgat tttatcgacc tgatacggcg catcgacaaa
    14041 ataagccgtt ttattgtgcg gctctttcgg gctgatatac cagatacgcg cattctgcgg
    14101 cgtcatcatc gccagacgtt cttttactgc tttagcatcg taccgatcgg caatattgac
    14161 tgcatccagc gtatgctcaa ccggaacgcg aatcatggta tctgccagcc attcgacgta
    14221 atccatatca cgggtgatcg acggataacg gaagtcgata tccagcacat tcgccagttc
    14281 atcgaagtat tgtttatcaa tgcctttttc acgtaacaga ttgagatagc taaaaattgc
    14341 cgccacaacc tgatcgcgat tagccaggcc tttatcggtt aaagacgcag agatcgctaa
    14401 tacgccgctg ttgccgttga cgataggatc ggagttggcg ctaatgccct caactaatcc
    14461 ctgcttttgc agccagtcgg aaagtgtacc tgggctgcga ttaccaatca gataggtaat
    14521 caattcatcc gttttactac ggaacttcgc tgagttgtta tcgatgcgaa actcaacgcg
    14581 cagaacttta cgcggcagtg ccgggacgta atgaatgata atgccctttt gcgcgtcggt
    14641 gactaccggc acggtgattt ccggtttttt gctctctttg ttcggcacgc gaccaaaggt
    14701 gtccgccgcc atttttgcca actccggcag cggtttattg ctgtaaataa ccgccttcat
    14761 cagattggcg gaatagtact tctcgtggaa atctttcagc gcctgctgca ccggattacc
    14821 aggtttatcg cttaaagttt cgaggttacc accagaaaac tttgaaccgg ggtgtgccgg
    14881 gttaatggtt tctgcgctga cctgtgccat gcgcatcccg tcacgcgtac gcgccatggt
    14941 taattcagcg ttcaccgcat tacgctcacg ttcggcatat ttcttgtcga gcaaaggttc
    15001 agcaatagca tcggccaggc ggtctaccgc accaggcaag gcgtcgttct caacttccag
    15061 atagaaagcc gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt gcattttgag
    15121 atattcggcc agactgtcag cctgcgggta ctttttcgac cccatcagac tcatatgttc
    15181 aaggtaatgt gccagcccct ggtacgcctc gggatcttcc agcgacccaa cgggcaccac
    15241 cagcgccgag agcgatttaa ctgcctgcgg atcagaaacc agcaagacca ccataccgtt
    15301 atccagacgt atagcctgat actggcggtt atctttatca cttttacgga tggtttcctg
    15361 aatcggctgc catcccgttt ctgcctgact taagggtgcc caaagggcaa ctaacaacaa
    15421 taatgctttg aaccaagtgc tgcggggcat tcacggacct cataatcaac ttaattttct
    15481 gtccagattc aacacgttaa cgcaaatcat ctgccagaat ttaatcttgt gctgcacgag
    15541 tcagcctatg tttatataac catcagtccg tgactggtgc gcatcataaa gtaagcggat
    15601 agattgcgca atttttatac agcactcatg actgattaaa gcgaaacagc ggtaacagga
    15661 aacgttgcga ctgttcaacg atggcctcca ttgtctctgg tgttaattgc cgccagagcc
    15721 tttgatacca gatattatca ccttcgccac gcaccatcat gttgccttcg taagcctgaa
    15781 ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc cagcatggca tcgttttgcg
    15841 cgtcataaca ggtttttagc cacgcgccgc cactttcagg taacaccagc aatggcgcgg
    15901 acatcccttc acgatacccc tcaatcagtt gtgagaggta atgcaaagcc tgttcggctg
    15961 caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt tcaccattac
    16021 caccgctggc acagtagaca aggtgttcca gccaaagttg cattccctgc gccacactta
    16081 ataaagaggg acgccagcgc aacaggccat ccggctgcac ctgcggcaac cagccagtta
    16141 tctgcacacc gttgcaggcg agatcaattt ccatactctg ccccggctgg cgacaggcaa
    16201 tgactctgtc ggcaagctgc tgcatctcct ggcactgagt ttcccagaaa atttcaccaa
    16261 aagcgccata cggtaaatcc cctgccgccc ggaagcgacg gaacaagcgt tcggcatcat
    16321 cctgctcaac cagtgcattc aataactgct gattgatttg ataacggcta agcccttcca
    16381 gaataaatgg ctcggtgtcg gggatttcgc tgtcttcggt acggaagttc acctgcaaac
    16441 gcatctggaa aaatgcccgc accggatgtg cccagaatcg ttgtagcgtt tccagcggca
    16501 cggtttccgg taaggtaaac ggcagcggct gaacaaattc agaatgtgct ttaccagcct
    16561 ggctggccgc aggtagccat tcacgagcat agctttgtcg ttcgcctggc tggtagtttt
    16621 gtggatcaaa cggcatccgg gtatggaggc aggtaagatg cgcttttacc cttgcctcgc
    16681 tttcatcaca gttgagcgct tcatcgcccg gtagataatg actttgcccg atgtagtcga
    16741 tcagttcctg caccagtacc gacgggaaac gttcactgtt atcctgaatg gaaagcccga
    16801 tataactgat atagagtttt tgctgcgcgg aaattaacgc ttccaggaac aggtagcggt
    16861 cgtcatcgcg acggctacgg tcgccacgct tcggtttctg gctcatcagg tcaaagccca
    16921 atggcgcaag ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa accactttga
    16981 acggaattga acgcattggc atcagagtac aaatgttaac cggtccggcg agaaaacgct
    17041 ggctgatacg ttcttgatcc agacgctgtg ccagttcatc acgcaatagt gacagcggca
    17101 ccgcgtcgcc atactgcgcc cctaaacctt cggcgataat cgcctgccat tgttgttcga
    17161 tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa gaaggcgttg agcatatcgc
    17221 gacaaaccgg caaccactct tctagcggac gctcctgtgc cagcccgcga cgccagatgt
    17281 ttagctgcat tagcagtgaa gccagatgcc ccaccagttc tgcaattaag ccgctcgact
    17341 catcataagg tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg tagcccagca
    17401 acatacgcgt caggccaaat cgccaggtgt gttgtccggt ggcggggagt tccagctcgc
    17461 gaacgttgtc gtcatctatc ccccaacgaa tgccggattc gttgacccac aggcgtaaat
    17521 aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag caccggcaca tccagtaatg
    17581 ccagcacgtc ttccgacaca aagcggctgt ctggcaacga taacaggctg ataaacgctt
    17641 ccagtaccgg atgcgactgc cgcgcccgac ggtcggaaat ggcgtaaggc aggtaacgat
    17701 ccgcaggtgc gctaccaaac acagcctgaa taaacggact gtagctgtcg atatcagcca
    17761 ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc ttccagcatc gccagcaggc
    17821 gatcgtgcaa aacttcaact tcacgctgcg ggctatggca aacgtggaag gtgatactgc
    17881 tatccagtgg atcaagcggg cgtttgttat cgctacggga aaactcttcg atgttcacac
    17941 cagcaacggc acggttttcc agttccagaa tgtcagactg aatgttatgc agcaggttat
    18001 ctggcgtgac atcgacaaag gcgtccagct cctggctgct ctccaggtca gaaaggagat
    18061 aaatgtagtc gcgcccaagc ttcccccatg aggccagcag cgggttgccg acatcctgtt
    18121 caccatcgct gttaaagagc tgtccggcat tttcgctatc gcgaaatagc ggcaattcgc
    18181 gatcttcaaa actgtgtcga cgctgacgag tcagcagttt cgccagataa gcaggatctt
    18241 taatatcgcc ccagtaataa cggcaggggt tggtaaacag gagatggatt tcaatatgtt
    18301 tacccagcgc ctggagcgcc tggagataaa caggcggtaa cgcggaaata ccgcatataa
    18361 agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt tcgataaagc
    18421 gctgatagag attcgcgcgg tgccagcgcg gttgtccgag ttcatgggta tattccacca
    18481 gcgccttcca caacggcgct tgccaggcct gtgcttctcc cagcccttca accaaatgtc
    18541 ctgtttccca ttgtgccagc cactccgggc gatagaccag atactggtca aacaggtccg
    18601 ccgcttttga ggaaagctgg aacagttttc gcttgtcgct atcgtcagtc agataatgcc
    18661 gcaacagggt aaagtcttcg tgctccaaca gttgcggcag cagagtcatc agtttccagc
    18721 tcatgctctg tttgttaaag gcgctctctt tggggatctc cggtaacacc cggacgaaca
    18781 tatcccagat aaagctcgca ggcagcggaa aatcaatgtt tgccgcaata ccaaactttt
    18841 gcgacagggt catttgcagc cactgtgcca taccggtact ttgcaccaga atcatctctg
    18901 gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac gcttccagca
    18961 cgtccagacg attggaatgg tagaccctta acatagcggc tcctgactac tgacgattcg
    19021 ggcaatgcag gcgggtcatc tcgccttctc tgcccccggg tgaaactagc gtaacgctga
    19081 tgctgacaca tcccgcctgc gatgtctgca atcggttgac ctgccagtta gctggtggcg
    19141 aaatcgcgcg cagttgcgtt tgctgccagc catgccgcca gagctgttgg tactggtttc
    19201 tgctggcaag actgttcatc aatgttcgct ggaaacccga taacgcagtg acaatcatca
    19261 ccatcaacac catcgccaac attacctccg gcaggctaaa gccttgttga ttcttcaggg
    19321 aagctgacat aacgccctct ctttcagcgg acaaaaatcg ctccagccgc ttggcgaaaa
    19381 aacaatgttt ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt cataaccggc
    19441 aatcaataag gcttcattat ctgccagtaa acgcaaacaa acctgggcat cggtttcagc
    19501 gtactgcgag cactgaactg ctggctgcgt ctgccaggag tgcatttttc cccacgccag
    19561 cgccgactga acgatggcct ggcggcgcaa tgactgactt tccatgctca cgcgagaggc
    19621 aaaactgcga tcctgttggc tcattccttg taatagcaaa ctacccaaaa ccagcagcat
    19681 caggaccaga gccagtgacg aaacaccctt ttcgcggttc acaggttgaa tcctgtcacg
    19741 ctatagctgg cattcaccac ggtttgcggt tcagacttac tggcagcacg catattaacc
    19801 gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt gtcgataatg
    19861 atggcatccg gattagtgac tttatcccag cccttacctt cacaggatgt cgcaccgcgt
    19921 agcgtttcca gcacatgctc cttcagacga aatccaatct ggtcggactc ttttaccggt
    19981 tcgcgatccc agataccgtt actgttcgca tcccactgca caatgataca gtcaccctgt
    20041 ccgacaattt ccagcccttc gccggtacag atgccatgac aataacccgc cctctggaga
    20101 tgcttcgcga cggtaaatac ccgcagccag atttcatctt ccagcgccag cttacgggtg
    20161 ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg cagcccccag caacaatacg
    20221 ctactgatcg ccatagcaat caacacttcc agcagagaaa aaccttgctc ttttacaggc
    20281 atccttctgt ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa accaccagcc
    20341 accactcgcc cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta ttgcgcaggc
    20401 caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag cgtggcacaa
    20461 agaccaatgg tgaactgcca tgacaggtat tggccccagc agcggaactc acaaggcacc
    20521 ataacgtccc ctccctgata acgctgatac tgtggtcgcg gttatgccag ttggcatctt
    20581 cacgtaaata gagcaaatag tcccgcgcct ggctggcggt ttgccatagc cgttgcgact
    20641 gctgccagta ttgccagcca tagagtccac ttgcgcttag catgaccaaa atcagcatcg
    20701 cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca gtatggagcg
    20761 aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg gactatttac
    20821 gccgttgcag gacgttgcaa aatttcggga aggtgtctcg aagaatttaa tggagggtaa
    20881 aaaaaaccga cgcacactag cgtcggctct ggcaggatgt ttcgtaatta gatagccacc
    20941 ggcgctttaa tgcccggatg cggagcgtag ccttcaatct caaaatcttc gaaacggtag
    21001 tcgaagatgg attcgggttt acgtttgata atcaacttcg gcagcggacg cggttcgcgg
    21061 cttaattgca gatgagtttg atccatatga ttgctgtaca ggtgcgtgtc gccccccgtc
    21121 cagacaaaat cgcccacttc cagatcacac tgctgcgcca tcatatgcac cagtaacgcg
    21181 tagctggcaa tgttaaacgg caggccgagg aaaacgtcac aggagcgctg ataaagctgg
    21241 caagagagtt tgccgtctgc cacatagaac tggaagaacg catggcacgg tgccagcgcc
    21301 attttatcca gttcgcctac gttccacgct gaaacaataa tgcggcgcga atccgggtcg
    21361 tttttcagct ggttcagtac cgtagtgatc tggtcaatat gacgaccatc cggcgttggc
    21421 caggcgcgcc actgtttacc atacactggc ccgaggtcgc cgttttcatc ggcccattcg
    21481 tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt tagtgtcgcc ctgcagaaac
    21541 cacagcagtt catggatgat ggaacgcagg tggcaacgtt tagttgtcac cagcgggaat
    21601 ccatcctgca ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc ggttccggta
    21661 cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa atactgtttc
    21721 atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac catcatgatc
    21781 acacccgcga caatcatcgg gatggaaaga atttgtccca tgctgatgta ctgcacccag
    21841 gcaccagtaa actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg aaacgcgccg
    21901 taaccaatca ggaacaaacc tgagacagct cccattgggc gcggtttacg aatatacagg
    21961 ttgaggataa taaacagcac tacaccttcc agcaacagct cgtaaagctg tgatgggtgg
    22021 cgcggcagca caccgtaagt atcgaaaatg gattgccact gcgggttggt ttgcagcagc
    22081 aaaatatctt ctgtacggga gccagggaac agcatggcaa acgggaagtt cgggtcaacg
    22141 cggccccaca attcaccgtt aataaagttg cccagacgcc cggcaccaag accaaacgga
    22201 atgagtggtg cgataaaatc agagacctgg aagaaggaac gtttagtacg gcgggcgaag
    22261 ataatcatca ccacgataac gccaatcagg ccgccgtgga aggacatgcc gccgtcccag
    22321 acacggaaca gatacagcgg atcggccata aactgcggga agttgtagaa cagaacataa
    22381 ccaatacgtc caccgaggaa gacgccgagg aagcccgcat agagtaagtt ttcaacttca
    22441 tttttggtcc agccactgcc cggacgattc gcccgacgtg ttgccagcca cattgcaaaa
    22501 atgaaaccca ccagatacat caggccgtac cagtgaagcg ccacgggtcc tattgagaaa
    22561 atgaccggat caaactccgg aaaatgcaga tagctactgg tcatctgtca ccacaagttc
    22621 ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt caggcgctcc
    22681 aaaggtgcga ataatagcac aaggggacct ggctggttgc cggataccgt taaaagatat
    22741 gtatatgatc cgcgctataa ccctccgcga atcagcccgc ccatgccgcg acgctccata
    22801 aaggctacaa cctgatgacg aacttcggtc gccagttgcg cttccagact acgctgcgca
    22861 agattttctg cttcggcaaa atcaatgcgc cgcagcaggt attttacccg cgctacagaa
    22921 cgtccgttca tagacagatg gcgataccca agcccaatga ggattgccac gcacatggga
    22981 tcgcccgcca tttcaccgca caaacggaga tcgataccat gtatttccgc ttcccgggcg
    23041 atcattgcca gagctcgtaa cattgcagga tgaaggctgt cataaatgtt cgccacccgg
    23101 gtattgttgc gatcaacggc cagaatgtat tgagtcagat cgttggtgcc aacagagatg
    23161 aaatcgaccc gctttgccag atgcggcagc ataaatacca ttgacggtac ttccagcatg
    23221 atgccgatac gtggtttggg aatttcgtaa ccgatcatct cctcgacttc acgtccggca
    23281 cgttcaatca ggcggcgtgc ttcgtcaact tcatcgaggc tggtgaccat cggcaacaga
    23341 atattcaggt tgcccgtagc ggcattagca cgcagcatcg cccgcacctg gatcaagaag
    23401 atctccggct gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg attctcttcg
    23461 ctgatcggca tgtaaggcag ctgcttatct gctccgacat ccagcgtacg caaggtgacg
    23521 ggtttatcat tgaacatttg cagcatcccc tgatactgcg ccacctgttc ttcttccgac
    23581 gggaaaccac tttgcagcat gaatgggatt tcagtgcgat aaagaccgat gccatcaata
    23641 cggctgccca gtttttcttc atgttccggg cttaaaccag cattgagcat gacttttata
    23701 cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat cttccgccag acggctaagc
    23761 tcaatctctt cactaattag ccgctgatat tcttgcagca gtatcggctc cggatcgacc
    23821 agcaattcac cgcgatagcc atcaacgatc agcgtcctac gatgcagcac cgaaggctga
    23881 atatccgcgc ccatcacggt agggagcccc agcgcacgta ccatgatcgc agcatgggag
    23941 ttggctgcgc catctcgcac gacaacaccg actaagcgat cctggggcag ctcagcaaga
    24001 gttgtcgctg acagttcatc tgccaccaga atgaaacgtt ccggccaggc gttcggccct
    24061 tgattagcgt catcaagatg aaacagcaat cgctgaccca gcgcacgtaa atcgccagcc
    24121 cgctctttga gatagttatc gcttagcgcg gcaaactgtt cggcaaattt ttcaatgacc
    24181 gtttttaccg cccactctgc caccgagcct ttatcaacct cggcaaacaa ttcgcgacgc
    24241 agccgggtgt ccgaaagcag gtgcgagtaa agatcgaaaa tagccgccgt ttctttttgt
    24301 gctccggcgg caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc ttccagcgcc
    24361 ccggtcaatc gttcgcgttc cagagccgga tccagcgttg atgcctgata cacctgttcc
    24421 attaaaggta acgtggcatc ctgccagcct tcggcaatcg ccacaccagg tgctgccggt
    24481 aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaagg cagtcaactg cgactgagaa
    24541 agaatagctg ccatctgggt ggcaagcgtc accaggaagg attcttcact ttcgtcatac
    24601 tggcgcaact ctcgttgctg taccaccagt acaccaagca actggcgacg ttgaataatt
    24661 ggtacgccta aaaacgcgcg gaaacgttct tcttttacgg aggggatgta tttgaagctg
    24721 gggtgctttt gcgcatctgc aaggtttatc ggttccgcca gcctgccaac caggccgacg
    24781 atcccttcat caaacgcgag cgttacagtg cgaccgcgtg gttttttcag cccccgggtc
    24841 gccatcaggt agtaacaacg tcgatcatga tcggccaggt agaccgaaca gacctcggta
    24901 tccatcgcaa gacagatgtc ggtaaccaga atatttaacg cctcattcag gcgtggtgcg
    24961 ctggctacct tttcgactat ttcgcgcagg cgagtgagca taattggcgt gacttaacct
    25021 cttttacgtc gataagcaga tgtgttttgt ggttttggcg tattttcctg cagtgacatc
    25081 accacactcg cgaactcttt cattacccta cggtagacat cacgtttaaa tgacaccacc
    25141 tgtctgaccg gataccagta acttacccat cgccagccgt caaactctgg tgtactgctg
    25201 gtttgcatat tgatttctgc atcgccgctc accagctgca agagaaacca tttttgtttt
    25261 tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa tttgtagcgc
    25321 aaccagttac gcgttgaagc aaggattcga acgtctttgc ggcttaatcc tacttcttca
    25381 aacaattcac ggtacatcgc ctgctctgcg gattctccgg ggttgattcc gccttgcgga
    25441 aattgccagg agtgctgacc aaatcgccgg gcccacatta cctgcccctg gcgattacaa
    25501 atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact acctcaaaat
    25561 aaagctttat atacgaatga ttgtttcata ctccaggaag acggtaaacc actctctgca
    25621 gggcattaca cactaataac aattgaataa ctcacagtta tgtgcagagt tataaacaga
    25681 ggaaggggtg gatagccgtt tttattcacc ttttctgtgg atagagttgt gaagaactac
    25741 ggaattacta cgggaaaacc cggagcattc tgaataagcc cgatataggc attttaaaat
    25801 attcattata agtcaatgca ttacatgtaa tttgagtaat gaaaaagagt aattcgtgac
    25861 ccaggtcaca cctctcattt acgggttggc gaaagatcaa ccaatgccgt atttatccac
    25921 agaatgtgcc actaagttaa gcactgaacc actaaaaact ggagtttcgt cgcacgtcaa
    25981 ggctgtaaat ggaaacagta gtggaggttt ttcacagtta tcccagcttt ctgtggataa
    26041 catggtgtaa gatcctgttt attttcagtg accagatttg gaaaacctat tgcagtgctg
    26101 cgcaactgga ttatcggcag cctaaaaagc aatataaatc agagaattga acaacacatg
    26161 tggaaaaagt tacactgcga atattcggta cataattgct gtttgttttt taaccaaggt
    26221 atcatgacat gtcccaacct cgcccactgc tctctcctcc cgaaactgaa gaacagttgt
    26281 tagcgcaagc acagcaactt tctggttata cattgggaga actggcggca cttgccgggc
    26341 tggttacgcc agagaattta aaacgcgata agggctggat tggcgtgtta ctggagatct
    26401 ggctaggtgc cagcgcaggg agtaaacctg agcaagattt tgctgctctg ggcgtggaac
    26461 ttaaaactat ccctgtggat agtcttggtc gtccgctgga aacaacattc gtttgtgttg
    26521 ccccgttaac gggtaatagc ggggtgacct gggaaaccag ccacgtgcgc cacaagctca
    26581 aacgcgtact gtggataccg gttgaaggcg agcgcagcat cccgctggcg cagcgtcgcg
    26641 taggatcccc gttgctgtgg accccgaatg aagaggaaga ccgacagcta cgcgaagact
    26701 gggaagaatt aatggatatg attgttctcg gtcaggttga gcggatcacc gctcgtcacg
    26761 gggaatattt acagataaga ccgaaagcag cgaatgcgaa agcgcttacc gaagccattg
    26821 gtgcccgggg cgaacggatt ctgacgctgc cacgcggctt ttatttgaag aagaatttca
    26881 ccagtgcact actggcccgt cattttctga tccagtagcc atcgctttga cctgccgctt
    26941 tccgggcata taattaccgc ttcatttttt tggcagggct ttttagatgt tatttgcatg
    27001 gataaccgat cctaacgcct ggcttgcgct cggtacgctg acgctgctgg agatcgttct
    27061 tgggatcgac aatattattt tcctttctct ggtggtggca aagcttccca cagcacaacg
    27121 tgctcatgcg cgccgtctgg ggttggcggg agccatggtt atgcgtctgg cgctgctggc
    27181 atcaatcgcc tgggttacgc gcctgacaaa tccgcttttt acaatattca gtcaggaaat
    27241 ttctgcccgt gatttgattc tgcttctggg tggcttgttc cttatctgga aagccagcaa
    27301 ggaaatccac gaatccattg aaggtgaaga agaagggctg aaaacacgcg tttcatcatt
    27361 cctcggcgct atcgtgcaga ttatgctgct ggatattatc tttagcctcg actcggtgat
    27421 tacggctgtg ggtctgtcag atcacctgtt tattatgatg gcagccgtgg tgattgccgt
    27481 aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt gtcgaacgcc atccttcggt
    27541 aaaaatgctg gcgctctctt tcctgattct ggtgggcttt accctgattc tggaaagttt
    27601 cgacatccac gtaccgaaag gttacatcta cttcgcgatg ttcttctcta ttgcggttga
    27661 aagcctcaac ttgattcgca acaaaaagaa tccgctctga tactccgtac gctctcctgc
    27721 gggagggcgt actcactcct tttaccctca cagtttaaga ttttcctgct ttcaagatat
    27781 ataacgtcgt tttataaaca gactattatc ataggtaggc caaatgagga taaccggatg
    27841 aaaaaatggg cagtaataat ttctgcagtc ggactggcgt ttgctgtttc cgggtgttcc
    27901 agtgattacg tcatggcgac caaagatggc cgtatgattt tgaccgatgg aaaacctgaa
    27961 attgatgatg ataccgggct ggtgagttat cacgatcagc aaggtaacgc gatgcaaatt
    28021 aaccgtgatg atgtttcgca aattattgaa cgttaacaaa taaggtcagc atccggctgg
    28081 ccttaagatt tttctctttc ccttttccct tccctctgcc atttttatat tccttatgtc
    28141 gtgattataa aaaggaaacg gctatgcaat atcaccgtat accccacagt tcgctggaag
    28201 tcagcacgct ggggcttggc acgatgacgt ttggtgaaca gaacagcgaa gccgacgccc
    28261 acgcacaact cgactatgcc gtcgctcagg gcattaacct tatcgacgtt gccgaaatgt
    28321 acccagtacc tccgcgcccc gaaacgcaag ggttaaccga aacctacgtc ggcaactggc
    28381 tggcgaaaca tggcagccgc gaaaagttaa ttatcgcctc caaagtgagc ggaccgtcgc
    28441 gcaataatga caagggcatc cgcccggatc aggcgctgga tcggaagaat atccgcgaag
    28501 cgctgcatga cagcctcaag cgcctacaga ctgattacct caatctttat caggtgcact
    28561 ggccgcagcg cccgaccaac tgcttcggca aactcggtta tagctggacg gattctgcgc
    28621 ctgcggtttc gctgctggat acgctggacg cactggcaga gtaccaacgc gcgggaaaaa
    28681 ttcgttatat cggcgtgtcg aacgaaactg catttggcgg aatgcgctac ctgcatctgg
    28741 cggacaaaca cgatctgccg agtattgtca ccattcagac cccttacagt ctgttaaacc
    28801 gcagttttga agtaggtctg gcagaagtca gccagtatga aggggtcgaa ctgctggcct
    28861 attcatgcct gggtttcggc acgctgaccg ggaaatatct caatggtgca aaaaccgctg
    28921 gcgcacgtaa tacgctcttt agtcggttca cccgctatag cggtgagcaa acgcaaaaag
    28981 ccgtcgcggc gtatgttgat atcgccagac gtcatggcct ggaccctgct cagatggcgc
    29041 tcgcgtttgt acgccgtcaa ccgtttgttg ccagcactct gctgggcgca accacgatgg
    29101 atcagctgaa aactaacatc gaaagtttgc atctggagtt aagcgaagac gtattagctg
    29161 aaattgaagc ggtgcatcag gtttatactt atccggcacc ataagattaa cgccggttaa
    29221 aaccggcgtt aaatattaat gacggcgctg ccagatccac agcgccgtta ttgccagcgc
    29281 aaacagcgca ccgaagccaa tgccaatggg cacgaccggg atgcctacca ttaccgccag
    29341 cgagtaaatg cccagcatca acaacatggc gctgttttcg ccaaggtttt gtactgcaat
    29401 cgcattcccc gccccgacgc tttttttacc ccgctcctgt agcaacgcat tgagcggaac
    29461 gacaaaaaag ccccccatca cgccaatcag catcaacaag gcataggctg gcagcagctc
    29521 gtgttgcagg gaaaaaatca gtaccaccac gccaatcaaa atcccggctg gcatacagcg
    29581 tgacacggtt tccagcgtaa ctaacttcgc tgccgcacct gcgccaacca cgataccaat
    29641 cgctaccatc gcgttgagat aggtgggcgt agcgttatcg gtaatgccca gcgccaccgg
    29701 tacccacagc accaacagga aacgcagcgt gacacccgct ccccagaata aactggtgcc
    29761 caccagcgaa aaacgcgttt caccattgcg ccatagcgag gtgcaggcat tcaggaaact
    29821 gcgggtcatg ttgatgagat tccaagactg ccccggacgc gccgccgcca gtttgggaat
    29881 gtagatattg gcaacgaccg caccaccgta ggccagtgcg catgcggcca gggcgacgag
    29941 gacatgccag tcagccagca caccaccggc tacggaaccg agcaaaatcg ccgctatggt
    30001 agaagcttcc attaaaccgt tagctttcac taacttacta cccgtggtta attcgccgag
    30061 aataccgtat ttcgccggtg aataggctgc agcaccaacc cccaccagcg tatagccgag
    30121 aaacggattg ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa
    30181 catcatcacc cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgcaaaaag
    30241 aatgtaagca cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg
    30301 cgctttcagt aacgccagag tggcaaacag tagggcatta tcgccaaacg cagagagaaa
    30361 ctgcgccacg ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga
    30421 ctcactcatc gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt
    30481 tgccgctgcc aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct
    30541 ccggcacgcc gtgctcgcgg gcatactgtt gcaacttatc gagcgtcagt tcgttatctg
    30601 tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga
    30661 ctttatctgg cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc
    30721 ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa
    30781 aacgcacaat atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg
    30841 cggtgggcac ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg
    30901 gccctttcag ttgcaggcgt ccgccctctt cgataccagg aaccgacaac aggcgcgcat
    30961 ccattcctgg tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag
    31021 aaacgacagg cgcgcactcg gttacaccat agccttcaag gatgcgcagg ccaaatttat
    31081 cctgccaaag ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta
    31141 gacgatagaa gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac
    31201 cgaacaacac ggtgcaactg cggtcataca ccaactccgg cacaatgcgg taatgcagcg
    31261 ggcttggata aaggaacact tctgcacctg taagcagtgg cgtaaacagg cctaccgtca
    31321 gcccaaagga gtgaaacagc ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg
    31381 caatcgtttt aatctgctcg acgttcgcca gaatgctttt atggctatgg acgacgcctt
    31441 ttggatggcc ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt
    31501 ttacctgcgc caggcgcggc atcagcaaat gagcgaagat ccatactttg tcggcagtgg
    31561 tgacatctgc ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca
    31621 gatgccagag tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag
    31681 ccgccgtaat agcactggtc agccctttta ccccagcagt gtagttcatc attgcgggaa
    31741 tgcggcgacg cgcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca
    31801 ttaagccgat gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg
    31861 ttttcgtaag caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct
    31921 tcccggctcc gaagcggtac attgcactca gtaaagattc gtacagagtt tcacgcgggc
    31981 gcaccgccat acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac
    32041 ggtcacgggc acgcgacgca tccggcatcg ccacctgcgt tggaggcaaa atatgcagag
    32101 taatttgcgg gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt
    32161 ccgccccttc aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg
    32221 cgccatcgta gattttcatc agcgagcctg tagtggtgat gcgtccttca gggaaaatca
    32281 ccactggtcg gccctgttcc accagacgta ccagatgttt aatagccata ggttgcgtcg
    32341 ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta
    32401 ttgaggtgta aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat
    32461 caataaaaga gacgtgatta ggcgtaatta gaacgcgctc gcccttcagt gcctgggtgt
    32521 cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca
    32581 ttccaactcc ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa
    32641 gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg
    32701 caagagacag ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatca
    32761 ccactttagc aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa
    32821 gtgtaaccaa aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt
    32881 cccacgatga aaacacgcca ctccttgaac caacgggcgt tttccgtaac actgaaagaa
    32941 tgtaagcgtt tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg
    33001 caggcgtttc agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag
    33061 cttcccggct ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg
    33121 cccgtgcgct ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg
    33181 atccgttttt cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt
    33241 tcttattgat tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac
    33301 tgatccgcca tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg gatgccgatt
    33361 tagcctcatt aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct
    33421 ttgaaaaccg ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt
    33481 taattcagca aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg
    33541 ccgaagatcg tctgcaaggg tattacgatg cccttgctga aagtggtatt gcggccaatg
    33601 accggctggt gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc
    33661 ttttgggacg aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg
    33721 gtgcgatggg cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg
    33781 gctttgatga tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc
    33841 caatcgtgac gatggcgacc caggctgccg aactggcttt ggcgctggcg gataatcgcc
    33901 ctctcccgga aatcactaat gtctttagtc cgacgctggt acgtcgtcat tcagtgtcaa
    33961 ctccgtcgct ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc
    34021 agcgccagta attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca
    34081 aacagaactt ctggtaacag cgaacggcta ttgtagttgg atgacattga tgcgccatat
    34141 gcccctgtat catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt
    34201 tcaacatttc ccccttcctg ctgggtaaag acatcgcccg attcacataa cggtccggcg
    34261 acgacggttt ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg
    34321 atatggtggt aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc
    34381 agcacaaagt ggcggctccc catttgtttg acgctccgca cctgagtaat taatacgcca
    34441 gactgcgcta ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg
    34501 cgggcgattt gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc
    34561 tcttcaccct gttgataagg aacagaaagc ccaccgcccg cagaaatagc ctgtaaatcc
    34621 tgaccgaatt cgatgacctg acgcaccata gcaccacaca cctgttccag atgggcataa
    34681 tcaacgccag aaccaatgtg catgtgaatg ccgaccagct gcagatgatg acgttgtatc
    34741 acgtccagtg cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg
    34801 gtattggttt tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga
    34861 tgccctggcg aaacctggcc cagttggtcg agcatatcaa cagaacccgc attcaccgga
    34921 atttgcaatt cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca
    34981 atatcatcgg ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct
    35041 aacgagacgg aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg
    35101 gaacaggctt tctgtgcaaa gcgcaccaca tcaaactgtt tcagcgctgc aatctgccga
    35161 cgaataattt gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc
    35221 agattttcgg cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc
    35281 agataagtgc ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat
    35341 gtcgagtcta tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc
    35401 gttaacttac gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag
    35461 gcggcacacc tgctacacac ctcacagcca accgtcagcc gcgaacttgc gcgctttgag
    35521 aaggtgatcg ggctgaaatt gtttgagcgc gtacgtgggc gattacatcc taccgtgcaa
    35581 ggactgcgtc tgtttgaaga agtgcaacga tcctggtacg gactggatcg aattgtcagc
    35641 gctgcagaaa gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc
    35701 ttttcgcaat cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc
    35761 agcttaaata tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt
    35821 catgatttag gactcactga gacactccat acgcctgcgg gaacagaacg taccgaatta
    35881 ctctctttag atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta
    35941 ttaacgccgg atgattttca gggtgagaac tacatcagcc tttcccgtac tgacagctat
    36001 cgccagttgc tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa
    36061 acccacagcg ccgcgtcaat ctgcgcaatg gtacgggcgg gggtaggtgt ttcggtggtt
    36121 aacccgctca ccgcactgga ttatgcggca agcggtttag tggtgcggcg gttcagtatt
    36181 gcggttccgt tcacagtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtg
    36241 caggcgttta gtgagcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct
    36301 attttgtcgt cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcag
    36361 tatcaaacac aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc
    36421 caaaaatgac gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag
    36481 cgcgtgacgc ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt
    36541 tcgcccgttc atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc
    36601 cgcgataaaa atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc
    36661 cggccccggt aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac
    36721 gtgactcaat ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg
    36781 cgcccgcccg ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt
    36841 ccccgcgacg ctggcactct tctatttcat gaaaatcgac gctatgtagc agcacttgcg
    36901 cagagtgaag cccaccaagc cgctgtttaa tgccttcatt tatcaaacga tagtaaggaa
    36961 tggtggattc ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat
    37021 gtgtgttacc gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaagtgcc
    37081 caatctctat taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagat
    37141 gccgatattt ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac
    37201 atttttggtt tccggaatga gccagaaagt gatgcccaca aacgcaatgt tcagcgcagt
    37261 gtagagccag aacgtaccgg cagcgccaat gctgtcgagc agcgtcagga aggtcgccga
    37321 taatcatatt cgacacccag ttcgtggtgg tcgaacaggt aataccgaaa tcgcggcatt
    37381 tcagcggctg aatttcagag cacaggatcc acaccactgg cgcggcgctc atcgcataac
    37441 cggcaataca catcatcgtc atgccaacag agagccagga caatccactg gaagccgtac
    37501 cgttatcaaa ctgcatcagg caatagccca gcaccagagt gcctaacgcc atcacgctga
    37561 aaccaatttt cagagccggt ttacccctgc tttatctacc gtaaacaccg caataaaggt
    37621 ggcgaacata aaggtcagcc ctacgaccag agtcgcaatc atctgttgtt ctgtggtcgt
    37681 aaagcccgcc attttgaaga tacgcggcgc gtagtacatg atgatgttca taccggtaaa
    37741 ctgctgcatc gcctgcaaca acataccgag aaacacagca cgacggacgt tacggttgat
    37801 cttaaacagt gcccaaccgc cctgttttaa cttcaggctt tcacgaattt cgttgagttc
    37861 ttctcgcgct ttttccgacg tatcgcgcag catacgcaat acttcttccg cctcaatatg
    37921 acgccccttt tccgccaacc agcgcgggct atttggcagg aagaccacca gaataatcag
    37981 cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc cagttaccgc tataactgaa
    38041 cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg accatcaact ggtacatact
    38101 gatcatctta ccgcgaacgt tttcacttgc catttcagaa agatacagag gagcggtgta
    38161 agacgcgatc ccgacagcaa tgcccagccc cacacgagcg gcgattaaca tctctacgct
    38221 ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg atggcccccg ccatcaggct
    38281 gtatttacgc cccaggcgga acgacagcca accattaaac agcgcaccaa ttgctgcgcc
    38341 gagcatcatg ctactaacca cccattcctg caaatgactg gtcagcacaa agtgatcggt
    38401 aatgaacggc aacgctccgg cgattacgcc gatatcaaga ccaaataaca atcctgcgac
    38461 cgcagcagct accgaaacaa acatattcat acgccgcgta tcccgcaaag aacgtggcgt
    38521 taaagcagat tccgtattga tagtaaccat tttttcctgc cagcagagag taagacatag
    38581 tgaaaaaata cgtgaacaac tcacgcaggt gtcaggtcgg aaacagcata aatatggatt
    38641 aaattgctgc gacatgtcgt tatgtgatgg atattccaat tttcaaatta agttgaatta
    38701 ttgagattat tattaaccac ctaattttac agcagataaa attcataaag ttcattaatt
    38761 gataattaat atggattatt tcataaccat gatatggatt atgatgatct acaggtataa
    38821 aaaaccctgc catgcggcag ggtcataaaa gtaagaagaa tgaattaacg cgccagccaa
    38881 ccgccatcca cggcaatggt ataaccattc acataatctg aagcgctgga ggcaaggaac
    38941 actatcggcc ccatcaggtc actcggcagt ccccaacgac cagctggaat gcggtcgaga
    39001 atttccgcgc tacgttgttc atctgcccgt agttgttgag tattgttggt cgccatgtaa
    39061 cccggagcta tcgcattaac attaatgttg tgtttagccc attcgttcgc catcaatcgc
    39121 gtcacaccca tcacgccgct ttttgatgcg gtataagaag gcacacggat cccgccctgg
    39181 aaggagagca ttgacgcgat attgataatc ttgccgccat tgccttgcgc gataaagtgt
    39241 ttcgccgctg cctgagacat gaagaatacg ctcttgatat tcaggttcat gacatcgtcc
    39301 cagtcttttt cgctgaactc gagagcgtct tcgcggcgaa tcaatccggc gttattcacc
    39361 aggatatcaa tatgaccaaa ttcagctacc gcgcgatcca gcagtccagg aatgccatca
    39421 atctttcgca gatcggcggt caggcttaaa aaacgacgcc ccagcgccgt gacctgctcg
    39481 atggtttcag tcggttcaac gatgttaatg ccaacaatgt cacagcccgc ttgcgccagc
    39541 cccaacgcca tcccctggcc cagcccagta tcacaaccag tgacgaccgc aactttacct
    39601 tcgagagaaa atgcacttaa aatcataaca ataccttatt cgtttatgcc cacaactagc
    39661 gcaaatcttt aacggccaca tggtccatat catcaaagac ctggttttca ccaaccatgc
    39721 cccagataaa ggtataagct ttggttccga cgccggaatg gatcgaccag ctcggggaga
    39781 tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc ttgcggttgc cccatcatgt
    39841 ggaaaacgca ggcgtcatca tccatattga aatagaaata aacttccatc cggcgctcgt
    39901 gggtgtgaca cggcatggtg ttccacaagt tacctggagc cagctccgtc agccccatac
    39961 tcaattggca ggtttccagc acatccggga caaaatattt gttaatagtg cgacggttac
    40021 tggtgaggtt atcgcctaac gtgactggag atacttcgtc cggggtgact tttttggtgg
    40081 gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt tgccggagtg gcggtatcaa
    40141 tactggcaaa gacaacttct tttgcacctt taccaacata cagggcgtcg cggtgaccga
    40201 tttcatagca ttggccatcg acagtaatcg taccggcacc gccaatattg ataacaccta
    40261 actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt accaacttcc ccgccaacgg
    40321 aaaccgtttt agttatcggc ataatgccgc caacaataat tcggtcaatg tggctgtaaa
    40381 ccatggtgta ctcatcggcg acaaatacct tttcaaccaa aaattcattg tgcagccctt
    40441 gggtatccag cgtttttgcg tgcgcactgt ggatgctctg tcttacgtcc acatcaacct
    40501 ccgataatca gtgaagcgtg aaaatagaaa acgatagtga aacactgttt cgttttctct
    40561 ataaagggat gatatgccca tgggctaggg ttttcaatta aaaataaaac aatgttttat
    40621 ttttaagtta aggattaaaa aaagtgcagt gtcgatcacg aatatgtcat gagaatggaa
    40681 aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc atcattattc aacagattaa
    40741 tgacggcaga tatttatcgg taaggaggaa atgcagagct actcgtcacg ttcaatggtc
    40801 aatgccacgc cctggccccc gccgatacaa agcgttgcca gccctttgcg ggcattacgt
    40861 ttcaccattt catgaaccag agaaaccagg attcggcaac cggaagcgcc tatcgggtga
    40921 ccgagtgcga tcgcgccacc attgacattg acccgacgct catcccactc aagcatcttg
    40981 ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct cgataagatc gacatcagcc
    41041 aactgccagc ctacacgctc caggcaacgg cgggtcgcat acaccggcgc aattcccatc
    41101 aatgccggat ctacaccaac gctggcaaat gcgcggatgc gggccagcac gggtaaattc
    41161 aacgctcgtg ctttggcttc gctcatcatc attaccgcag ctgcgccatc gtttatggat
    41221 gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg aaggatttaa acgggctaaa
    41281 ccttctgcac tggcgtcagt gcatggctgt tcatcggtat caacaaccaa cgtctgaccg
    41341 ttactttgga tcattaccgg gacgatctca tctttaaatc gtccggcgtc aatcgccgct
    41401 cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca actgacggct gatgccatat
    41461 tcgcgagcca gattttcggc ggtgacacca atatgataat cattgaaggc atcccacaac
    41521 ccatcatgca ccagactgtc aaccaactgg ctattgccaa gctgtgcacc ggtgcggcta
    41581 tcagtcagaa catgtggtgc gcggctcatg ttttcctggc caccggcgat gacaatatca
    41641 gcctcgccac actgtatcgc ctgagtagcc agatgcagtg ctttaagccc agaaccgcaa
    41701 acgtcattaa tagtgattgc agaaacgcta ttaggaagac cacctttaat agccgattgc
    41761 cttgccggat tctgccctgc ccctgcagtc aacacctgac caagaattac ttcatccacc
    41821 gcatatgcag gaacgccggt acgttctatt aacgctttca cgaccaaact accaagttcc
    41881 acggcggaat gacccgctaa cgcaccacga aagcagccga taggtgtccg taacgccccg
    41941 acaatcacaa cgtctttcat cacaacctcg caccaattaa cagtgcaata gtaaatgatt
    42001 gttaattaca gttatcttaa ttgtttaaaa aaagtgattt ttatcacaaa agaaatatgc
    42061 ctgagcagca gtcagagaca taactggcac gtaaggtttg caaccactaa cccaccaata
    42121 gaggggtaga tagggcgtta atctcccata cttaacctgg tttatggtaa attgccctcc
    42181 attttgttta atttgtagat gatacgttca gataatgtct aatatttggt ctaaagaaga
    42241 aactctgtgg agtttcgcgc tctacggcac agccgttggt gcaggcacgc tcttccttcc
    42301 tattcagtta ggttcggcag gggctgtggt cctgtttatt actgctctgg tcgcctggcc
    42361 tttaacatat tggccacata aagctttatg ccagttcatc ctctcatcga aaacatcagc
    42421 aggtgaaggg ataacgggcg cggtaacaca ctactatggc aagaagattg gtaatctgat
    42481 taccacgctg tacttcatcg ccttttttgt cgtcgtgttg atatatgcag tggcaattac
    42541 caactcactt acagaacagc tggcaaagca tatggttatt gatcttcgca tccgtatgtt
    42601 ggtgagtctg ggtgggtaat gactccaact tactgatagt gttttatgtt cagataatgc
    42661 ccgatgacct tgtcatgcag
//
LOCUS       DAJTSL010000010        41642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTSL010000010 DAJTSL010000000
VERSION     DAJTSL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.010"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..255
                     /locus_tag="OO433_000496"
     CDS             <1..255
                     /locus_tag="OO433_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6049092.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO433_000497"
     CDS             complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO433_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6049093.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO433_000498"
     CDS             complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO433_000498"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6049094.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2907..3182)
                     /locus_tag="OO433_000499"
     CDS             complement(2907..3182)
                     /locus_tag="OO433_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6049095.1"
                     /translation="MSRTIFCIFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO433_000500"
     CDS             complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO433_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6049096.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4423..5142
                     /gene="trmB"
                     /locus_tag="OO433_000501"
     CDS             4423..5142
                     /gene="trmB"
                     /locus_tag="OO433_000501"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6049097.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLNNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5142..5468
                     /locus_tag="OO433_000502"
     CDS             5142..5468
                     /locus_tag="OO433_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6049098.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5652..6371
                     /locus_tag="OO433_000503"
     CDS             5652..6371
                     /locus_tag="OO433_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6049099.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6547..7593
                     /gene="ansB"
                     /locus_tag="OO433_000504"
     CDS             6547..7593
                     /gene="ansB"
                     /locus_tag="OO433_000504"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6049100.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7710..8717
                     /locus_tag="OO433_000505"
     CDS             7710..8717
                     /locus_tag="OO433_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6049101.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQSAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OO433_000506"
     CDS             complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OO433_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6049102.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(10001..10594)
                     /locus_tag="OO433_000507"
     CDS             complement(10001..10594)
                     /locus_tag="OO433_000507"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6049103.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVTQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(10602..10892)
                     /locus_tag="OO433_000508"
     CDS             complement(10602..10892)
                     /locus_tag="OO433_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6049104.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OO433_000509"
     CDS             complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OO433_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6049105.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNILLPGMWMAL"
     gene            complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OO433_000510"
     CDS             complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OO433_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6049106.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            12195..13175
                     /locus_tag="OO433_000511"
     CDS             12195..13175
                     /locus_tag="OO433_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6049107.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDALDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OO433_000512"
     CDS             complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OO433_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6049108.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(13767..14330)
                     /locus_tag="OO433_000513"
     CDS             complement(13767..14330)
                     /locus_tag="OO433_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6049109.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTASADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OO433_000514"
     CDS             complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OO433_000514"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6049110.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVLLMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OO433_000515"
     CDS             complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OO433_000515"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6049111.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OO433_000516"
     CDS             complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OO433_000516"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6049112.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(17015..17512)
                     /locus_tag="OO433_000517"
     CDS             complement(17015..17512)
                     /locus_tag="OO433_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6049113.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OO433_000518"
     CDS             complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OO433_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6049114.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OO433_000519"
     CDS             complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OO433_000519"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6049115.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATTFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(20877..21092)
                     /locus_tag="OO433_000520"
     CDS             complement(20877..21092)
                     /locus_tag="OO433_000520"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049116.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            21228..21359
                     /gene="yqgB"
                     /locus_tag="OO433_000521"
     CDS             21228..21359
                     /gene="yqgB"
                     /locus_tag="OO433_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6049117.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            21368..23344
                     /gene="speA"
                     /locus_tag="OO433_000522"
     CDS             21368..23344
                     /gene="speA"
                     /locus_tag="OO433_000522"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6049118.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            23481..24401
                     /gene="speB"
                     /locus_tag="OO433_000523"
     CDS             23481..24401
                     /gene="speB"
                     /locus_tag="OO433_000523"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6049119.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OO433_000524"
                     /gene_synonym="yggG"
     CDS             complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OO433_000524"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6049120.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            25643..27634
                     /gene="tkt"
                     /locus_tag="OO433_000525"
     CDS             25643..27634
                     /gene="tkt"
                     /locus_tag="OO433_000525"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6049121.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            27948..28391
                     /gene="cmtB"
                     /locus_tag="OO433_000526"
     CDS             27948..28391
                     /gene="cmtB"
                     /locus_tag="OO433_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR6049122.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISHG"
     gene            28419..29807
                     /gene="cmtA"
                     /locus_tag="OO433_000527"
     CDS             28419..29807
                     /gene="cmtA"
                     /locus_tag="OO433_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR6049123.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSLGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDTGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            29822..31099
                     /locus_tag="OO433_000528"
     CDS             29822..31099
                     /locus_tag="OO433_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6049124.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLVEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGIDIGEAAQ"
     gene            31096..32061
                     /gene="yggF"
                     /locus_tag="OO433_000529"
     CDS             31096..32061
                     /gene="yggF"
                     /locus_tag="OO433_000529"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6049125.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            32083..32592
                     /gene="fumE"
                     /locus_tag="OO433_000530"
     CDS             32083..32592
                     /gene="fumE"
                     /locus_tag="OO433_000530"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6049126.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTILKELTP"
     gene            32589..33302
                     /locus_tag="OO433_000531"
     CDS             32589..33302
                     /locus_tag="OO433_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6049127.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(33274..33951)
                     /locus_tag="OO433_000532"
     CDS             complement(33274..33951)
                     /locus_tag="OO433_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6049128.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(33945..34622)
                     /locus_tag="OO433_000533"
     CDS             complement(33945..34622)
                     /locus_tag="OO433_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6049129.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(34610..35317)
                     /locus_tag="OO433_000534"
     CDS             complement(34610..35317)
                     /locus_tag="OO433_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6049130.1"
                     /translation="MHPFTSLILWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(35318..35896)
                     /locus_tag="OO433_000535"
     CDS             complement(35318..35896)
                     /locus_tag="OO433_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6049131.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(35919..36350)
                     /locus_tag="OO433_000536"
     CDS             complement(35919..36350)
                     /locus_tag="OO433_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6049132.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYARQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            36722..37741
                     /gene="epd"
                     /locus_tag="OO433_000537"
     CDS             36722..37741
                     /gene="epd"
                     /locus_tag="OO433_000537"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6049133.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            37791..38954
                     /gene="pgk"
                     /locus_tag="OO433_000538"
     CDS             37791..38954
                     /gene="pgk"
                     /locus_tag="OO433_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6049134.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            39169..40248
                     /gene="fbaA"
                     /locus_tag="OO433_000539"
     CDS             39169..40248
                     /gene="fbaA"
                     /locus_tag="OO433_000539"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6049135.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLSFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            40606..41466
                     /gene="mscS"
                     /locus_tag="OO433_000540"
     CDS             40606..41466
                     /gene="mscS"
                     /locus_tag="OO433_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6049136.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
BASE COUNT        10193 a        10411 c        11090 g         9948 t
ORIGIN      
        1 attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat tgcggcagaa
       61 ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt ctggggtggg
      121 gcggttcaac gttatttcct tgcactggaa gaaggggtaa atttgttgcc gggattttcg
      181 ccggagctgc aaggtgttta tagcgaaacc gatgcggatg gcatgaaacg gttgtacggt
      241 tatgtgttga agtaagaata aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc
      301 gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta tatttgaaca
      361 tcgccatgaa cgcgaaggcc agaaccacgg agtaaccagc gaaaatcaac catacggtct
      421 gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc acgatgccgc
      481 cgaggataca gccgaagccg ttagtcatca tcaggaacat cccttgtgca ctggcgcgaa
      541 ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag aagtcgaatg
      601 cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga gtcgggtcgc
      661 cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc attacgttct
      721 taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt tcagagatct
      781 gcgaaatcga catgatgatt gacgcatgct gcacaataaa gctgctggca aacatcggat
      841 ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc agttccgcgc
      901 ccagcagcat tgagaagata aagaagattg ccatacgctt gtttttaaac agcgcgaatg
      961 catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca accggaatat
     1021 gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca atatacagct
     1081 gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg ataaagccga
     1141 tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg gcattttgca
     1201 ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca aacgagttaa
     1261 tcaatatcac aaggaacatc gcctccggtg tcgtgacctg tgccgccatg aacagcgtga
     1321 tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat ttgtcggcca
     1381 caatccccag cagcgcaggc ataaagaccg ctgcgatacc cagtgagcta taaactgcgc
     1441 caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg gtcgtcagcc
     1501 aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc ttaagattca
     1561 tgttaatttc ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt tgaccgtgcg
     1621 agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg caaataattg
     1681 caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg cccctgagca
     1741 tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg gtattcactt
     1801 tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc tgataaacat
     1861 cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc gaaaagactc
     1921 gcagcacgct gcctgcgccg ccgttatagg cggtgatgac ggcataacgc cgcgatgttg
     1981 ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg gtgccggtat
     2041 caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg
     2101 agcggaacac atctttcccg gcagtatgtt gtaccacctg cattaatccc agcgcatcgg
     2161 aacggctgac cgcatacggg ttaaaggaag attcggtctg cataattgcc agaatcagcg
     2221 actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac
     2281 gtttatcaag gtggttcggt accatgttaa tggtgacgct gtagatgata cgcagcccgt
     2341 tactgcggct ttgcagacgg tttttcagca gataatccgc gaagttgctt gcgcgacctt
     2401 cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg
     2461 aaatcgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg tcacccatca
     2521 gcaacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg gcgatagttt
     2581 cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt
     2641 atttcacgta gtccttagga ccggcgatca ccacctcttt gaatccccag atgttctcaa
     2701 tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtatctttg acccaggctt
     2761 cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag
     2821 ccagcgcgag atattttttc atcattccgg gtgcgtgttg tgtttgtttt tatgcaagtt
     2881 gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata gccctcgata
     2941 tgcacctctt taccctcgaa caggaagttg accatctcct gctcaagcag cttgcggtgc
     3001 tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgctt gtgctgccac
     3061 tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc gccggggtac
     3121 agctgaaaat cctgaccttc tgcttcacgt tgcaggaaaa tacaaaaaat cgttctgctc
     3181 ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg taactgctgt
     3241 aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa gttataccag
     3301 agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca cataggcaca
     3361 atatctaagt ggaaatggct gaaggtatgc cgaaacgcgg tcagttgcgt caggttatcg
     3421 gcagcaatct gccgttgcgc cagccactgc cgcaaacttt cttcgtcggc aaactgcggg
     3481 aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa tacttcatct
     3541 tcgtgctgta acagcaaaaa gtagccggtg cgctccggca gcgtctgttt cggttttttg
     3601 cccggataaa gcgaccagct attattggcg gcggcaatac atccgttttg tagcggacag
     3661 agcgaacatt tcggcttcga gcgcgtacaa atcatcgcgc ccaaatccat catcgcctga
     3721 ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc tcaaactcca taatttattc
     3781 tcgacctctt ttttcccagg ccagccgctt acagcatagc agcgcgccag cacgcgtttg
     3841 acgttaccgt cgagaatcgg aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg
     3901 gtggaacgcc cgacgcccgg taacgccgcg acttcttcaa aggtttccgg gaatttaccg
     3961 ccgtgtaagg tcgccacttg ttgtgccgct ttatgcagat tgcgcgcgcg ggcgtaatag
     4021 ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg cattggcgag atcggtcacc
     4081 gtcgggaagc gcgccataaa gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt
     4141 tgcaacatca cttctgagag ccatactttg tagggcgtct tgtcaatttg ccagggcagc
     4201 gtttttcgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc
     4261 atggtcaccg aattcactgt tgttgggggc aagattgcag cacagcgacg gcagggtgta
     4321 aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact aactttggat
     4381 aatgcccgtt ttcagaacac tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt
     4441 tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc
     4501 caggggcgac tgaccaaagg ccaggaacat gcgctggaaa actactggcc ggtgatgggc
     4561 gttgagttca gcgaagatat gctggatttc cccgcgcttt ttggccgtga agcgccggtg
     4621 acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa agatcgccct
     4681 gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct
     4741 gcgcatgaag aaggtttaaa caacctgcga gtgatgtgtc acgatgcggt tgaagtgctg
     4801 cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc tgacccgtgg
     4861 cacaaagcgc gccataataa acgccgtatc gtacaggtgc cgtttgccga actggtaaaa
     4921 agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc ttatgcggaa
     4981 catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga gagcaatgat
     5041 tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg tcatcgtctt
     5101 ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag aaccgtagcc
     5161 gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt tcggtggcat
     5221 ggcgattccc ggaaggtaca tcggaagaac agattgataa aaccgttgat gattttatta
     5281 acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg gcctgggaag
     5341 gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg attgtgcgta
     5401 agtggctgga agagcgcaaa ctggatgagg tacgcaccag cgaacttttc gacgtttggt
     5461 gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat
     5521 caggcctgga aagatgcacg atcgagtagg cgggataagg tgtttacgcc gcatccggca
     5581 tggaaaacgc gtactttgtt atcaatctgg ggccagcaaa tgctggcctg attttttctt
     5641 gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg acggcaatga
     5701 ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc
     5761 aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac ggcaacgtga
     5821 tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg aaggattatc
     5881 aggctgaact acgcagcacg ctgccgtgga ttgatgaagg cgcgaaaagc cgcgtcgaaa
     5941 aagcccgtat tgcgctggat aaaattatcg ttcaggagat gggcgaaagc agcaaaatgc
     6001 gcagccgtct gaccaaactt gatgcgcaac tgaaagagca gatgaaccgc attatcgaaa
     6061 cgcgcagcga tggcctgacg tttcactata aagccattga tcaggttcgc gccgaaggcc
     6121 agcaattagt gaatcaggca atgggtggaa ttttacagga cagcattaat gaaatgggcg
     6181 cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa tgtgctggga agcctggggg
     6241 ggctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc cagcagtttg
     6301 gcaaagatgt ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc ctggtcggga
     6361 atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt ttaattcttc
     6421 gttttgttac ctgcctctaa ctttgtagag ctccaaaata tattcacgtt gtaaattgtt
     6481 taacgtcaaa tttcccatac agagctaagg gataatgcgt agcgttcacg taactggagg
     6541 aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg ttttagtggt
     6601 gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat tgccggtggt
     6661 ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga aaatctggtt
     6721 aatgcggtgc cgcaactaaa agacattgcg aacgttaaag gcgagcaggt agtgaatatc
     6781 ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat taacaccgac
     6841 tgcgataaaa ccgacggctt cgtcattacc cacggtaccg acacgatgga agaaaccgct
     6901 tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg cgcaatgcgc
     6961 ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt ataacgcggt agtgaccgca
     7021 gctgataaag catccgctaa tcgtggcgtg ctggtggtga tgaacgacac cgtactggac
     7081 ggtcgcgatg taaccaaaac caacaccacc gacgtagcga ccttcaagtc tgttaactac
     7141 ggtcctctgg gatacattca caacggtaag attgactacc aacgtacccc ggcacgtaag
     7201 cacaccagcg atacgccatt cgatgtctct aagctgaatg agctgccgaa agtcggcatc
     7261 gtttataact acgctaacgc atccgatctt ccggctaaag cactggtaga tgcgggctat
     7321 gatggcatcg ttagcgctgg tgtgggtaat ggtaacctgt ataaatccgt gttcgacacc
     7381 ctggcaaccg ccgcgaaaaa cggcactgca gtagtgcgtt cttcccgcgt accgacgggt
     7441 gctaccactc aggatgctga agtggatgat gcgaaatacg gcttcgtcgc ctctggcacg
     7501 ctgaacccgc aaaaagcgcg cgtcctgctg cagctggctc tgacgcaaac caaagatccg
     7561 cagcagatcc agcagatctt caatcagtac taatcacctc gctccggtat cgtgccgggg
     7621 ctttttcact tcagacttac gtccattgcc aattttcatt accctaatga taatcaccgg
     7681 aataaattat ttcgcgtgag gtttttcggg tgaaaaagca atggattgtt ggcacggcac
     7741 tgcttatgtt gatgactggt aatgcctggg cagatggcga accgccaact gaaaatatct
     7801 taaaagatca attcaaaaag cagtatcacg gcattctcaa gcttgatgct atcaccttaa
     7861 aaaatcttga tgctaagggt aatcaggcca cctggtcagc ggaaggcgat gtctcttcca
     7921 gtgacgatct ctatacctgg gtcggtcagt tggcagatta cgaactgctc gaacagacct
     7981 ggacgaaaga taaaccggta aaattctcgg cgatgttaac cagtaaagga acgcccgcgt
     8041 ctggctggtc ggtgaacttt tactcttttc agtcggcagc cagcgatcgt gggcgggtgg
     8101 tcgacgatat caaaacgaat aataaatatc tgatcgtgaa tagcgaagat ttcaattatc
     8161 gctttagtca gcttgaatct gcgttgaata cccagaagaa ctcgattcct gcgctggaaa
     8221 aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca gaaagcagcg gatgcatatt
     8281 ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga agcctttaaa aaaattcacc
     8341 aacagcgtga tgagtttaat aaacagaacg atagcgaggc gttcgcggtt aaatatgaca
     8401 aagaggttta tcaaccggcg attgcggcat gtcataaaca gagtgaagag tgttatgaag
     8461 tgccgattca gcagaaacga gatttcgata tcaacgaaca acggcgacag acttttctgc
     8521 aatcacaaaa actcagccgt aaattgcagg atgactgggt aacgctcgaa aaagggcaat
     8581 atccgttaac gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt ttgatgaaaa
     8641 ttgacgatat caaccaagct aatgaacgct ggaaaaaaga taccgaacaa ctgcggcgca
     8701 atggggtgat taagtaagtt tgatatagtg cacgatttgt agcaggatgc ggcgttgacc
     8761 ccgcatccgg caatcaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgt
     8821 tgccacatgt aggctggata aggcgttcac gccgcatccg gcaatacaag tttactcagc
     8881 cagaaaaagc tccagcagcg aatttaaaaa cagcttccca tgttccgtta tctgccagta
     8941 atccgcacat tctgtgagat aaccctgggc aatcgcctcg tctaactgtg ggcgaatcac
     9001 atcttcgcaa agcccggtat acgcactaaa ctctgcgcgt ggcgcggcct ccagcaagcg
     9061 aaagcggttc ataaagaact caaacggctt atctgcggct tcgacatcac gctggctttc
     9121 cagatacctt ccttgcataa aaccacgagg atgacgcgtt ttagtggtac gcagaatgcg
     9181 cccatccggg aaggtgactt tgccgtgcgc gccgcagcca ataccgatgt agtcaccaaa
     9241 tcgccagtag ttgagattgt gctggcactg ataaccgggt ttggcgtaag cggaagtttc
     9301 atattgctga taacccgctg cggttaataa ctgatgcccc tgttcgaaaa tatcccacag
     9361 cgcgtcatcg tccggcagca ccggtggtcg cgaaccaaac agcgtattgg gttcgatggt
     9421 cagttgatac caggaaagat gcggcggatt cagttcaatg gcctggcgta gatcgccaag
     9481 cgcctcttcc agtgattgat ccggtagtcc atgcatcaaa tcaaggttaa agctacgtaa
     9541 ccctaaaccg ctcgccagct ttgccgctcg tttcgcttct tgcgggccat gaatgcgccc
     9601 aagtcgtttc agcttttctt cgctaaaact ctgcacacca atagagatgc ggttcacacc
     9661 agcacgctga taatcgacaa agcgatcggc ttctaccgta ccagggttcg cttccatagt
     9721 aatttctgca tccgctgcca gcggcaaacg cgcacgcacg ccgtccagca gcgtttgcat
     9781 cgccgggccg gaaagcaggc tcggcgtacc accgccaata aagattgtct ttacttcacg
     9841 gccctgagcg taagccacat cattgtccag atcgcaaagc agatgctgaa cataatcgtc
     9901 gtgcggcact tctcctttca acgcgtgaga gttgaaatcg cagtacgggc atttctgcac
     9961 gcaccacggg atgtgaatgt agagactcag cggcggtaat ttaaccatta cgtaaagcgt
    10021 ccagcagcag tttcaacgcc tgaccacggt gggaaatggc gctcttttct tcgcgggtca
    10081 gttcggcggc ggttttccct tcggaaggta cgaagaagat tggatcataa ccaaagccac
    10141 cagtgcccgc tggttcgcga gtaatcacgc ccggccagct gccgtggcat accagcggag
    10201 tcggatcttc cgcgtgacgc agatatacca gcacgcagtg gaaacgtgcc tgacgttggt
    10261 cgtccggtac gtctttcagt gtttccaaca gtttttgcag attcttttga tcggtcgcgt
    10321 cttcaccgga ataacgcgcg gagtaaatac ccggcgcgcc acccagcgca tctaccgcca
    10381 gaccagagtc gtcggcaatt gccggtaaac cggtcacttt tgccgcatgg cgcgctttca
    10441 gaatcgcgtt ttcgataaag gttaggccgg tttcttcagc ggaatcaacg ccgaggtctg
    10501 tttgtgtcac gatatcaaga ccaaagtcgc taagcagcga cgccagctca cgcactttac
    10561 cggcattgcc ggttgcgagg acaacttttt gcataggata cctaattaat taacgccgcg
    10621 atttctggcg ggatttgttg cggattaatg attttaattt gtttgtggcg gccaagttcg
    10681 cctttttcaa tcaccacctg gcttttggca acccggaatt gcttaccgag aaacttcacc
    10741 agatgactgt tggcctggcc gtcaactggc ggcgcggtaa tggcgacttt aacttcgtcg
    10801 ccatgtaaac cgacaataga atcacggctg gctttcggct gaatatagag ccgtaaaacc
    10861 agaccgtcat cattaactgt tacggcattc ataacgccat ccacatcccc ggcagcagga
    10921 tgtttccggt tgcctgtaat acttctgcga cacccatatt gatgacatac agcagcagaa
    10981 cgaggatcat cggcgagaaa tcaatcccac ccattgccgg tagcaagcgg cgaatcgggc
    11041 gcagcagcgg atcggccagc tgaatcagca cgtattcaat cgggctacga ccctggctta
    11101 cccagctcat aatcgccatc accagcagga cccagaaaat cagcaagccg atggttttca
    11161 gcagaatcag taaaccggca atccagatga ttggcaggaa ggtcaccact ttaaacagca
    11221 caatggcttt gataaaactg agaatatagg caaccagcag cgaggcgctg tcaatgggac
    11281 ccattgccgg aataacgcgg cgcagtggcc cgataattgg ctgcgttacc ttcactacaa
    11341 actgtgagaa ggggttgtaa aaatcacaat gagcccactg catccagatg cgtaataaca
    11401 gcaccatggt atacagctca atgaccgttg aaagcaggaa agtcaacgta ttcatggcgt
    11461 tcctttaatt ccttattttt tagagtaatc acgcgcacca aaaattgcag tgccgatacg
    11521 aaccatcgtg ctacctgccg caatggcggc ttccatatcg tccgacattc ccagagagag
    11581 cgtgtcgata tgcgggtagc gcgttttcag tccggcaaat gctacagcca tttggcgtgc
    11641 aacttcaaac tgccttacat attctgactc aggcgcaggg attgccatca gcccgcgcag
    11701 acgtaaacgc ggtagttcag cgaccgccgc tgccagctcg tccagttctg ccagttgaat
    11761 cccggactta ctgttttcat cactaatatt aatttgaatc agtacgttaa gagggggaag
    11821 ttctgccggg cgctgatcgt tgaggcgggt agcaatgcgc aaacggtcga tggtatgaca
    11881 ccagtcgaaa tgctctgcca ccaggcggct tttattagac tgcaacgggc caataaaatg
    11941 ccattctaat cctgttacgc ccagttcctg aaagtggcga attttatcta ccccttcctg
    12001 aacgtagttt tcaccaaatt gacgctgccc ggcatcaatg gcttctgcga tggcgctcgc
    12061 aggttttgtt ttactgactg caagcagcgt aatttcttct ggagaacggc cgcaacgcgt
    12121 tgcagccgct gagattttgt cccggacctg tgccaggtta tgcgcaatat cgttcatttt
    12181 ccgaggatct tagtatgaat atggaagaaa ttgtggccct tagtgtaaag cataacgtct
    12241 cggatctaca cctgtgcagc gcctggcccg cacgatggcg cattcgcggg ctaatggaag
    12301 ctgcgccgtt tgatgcgctg gacgtcgaag agctactgcg ggagtggctg gatgacgatc
    12361 agcgggcaat attgctggaa aatggccagc tggattttgc cgtgtcgctg gcggaaaacc
    12421 agcgattgcg tggcagcgca ttcgcgcaac ggcatggcat ttcgctggcg ttacggctgt
    12481 taccttcgca ctgcccgcag ctcgaacagc ttggcgcacc aacggtattg ccggaattac
    12541 tcaagagcga gaatggcctg attctggtga cgggggcgac ggggagcggc aaatctacca
    12601 cgctggcggc gatggttggc tatctcaatc aacatgccga tgcgcatatt ctgacgctgg
    12661 aagatcctgt tgaatatatc tatgccagcc agcgatgttt gatccagcag cgggaaattg
    12721 gtttgcactg tatgacgttc gcatcgggat tgcgggccgc attgcgggaa gatcctgatg
    12781 tgattttgct cggagagctg cgtgacagcg agacaatccg tctggcactg acggcggcag
    12841 aaaccgggca tttggtgcta gcaacattac atacgcgtgg tgccgcgcag gcagttgagc
    12901 gactggtgga ttcatttccg gcgcaggaaa aagatcccgt acgtaatcaa ctggcaggta
    12961 gtttacgggc cgtgttgtca caaaagctag aagtggataa acaggaagga cgcgtggcgc
    13021 tgtttgaatt attgattaac actcccgcgg tggggaattt gattcgcgaa gggaaaaccc
    13081 accagttacc gcatgttatt caaaccgggc agcaggtggg gatgataacg tttcagcaga
    13141 gttatcagca gcgggtgggg gaagggcgtt tgtgacgttg ccatgcccga tgcgcaagtt
    13201 tatagggcct ataaaggcaa taaatgtctg atgcgacgct aacgcgtctt atcagaccta
    13261 cagccgctgc cagatgtgtg ctggataagg cgttcacgcc gcatccggca agatggcggt
    13321 aacgactatc cgccaggctt ttagatcgcc ttaatatccc tgctcgaaat agctttcgag
    13381 aataataacc gcagaggcag agtcgacttt gcctttgttg agcgcccgat agccgccctg
    13441 ttcaaacaga ccggaacggg cttccacggt gctaagacgt tcgtcatgga gctttacttc
    13501 aacaccgaaa cgaccatgaa tacggttggc aaatttacgc gctctggcag tcaatggttg
    13561 ctcggtgccg tccatattca gcggcaaacc gacgatgatt tcgtccggct gccactcttt
    13621 cagtaaacgt tcgatgaggt tccagtccgg cgtaccgtcc tgtgctttaa ttgcaggcaa
    13681 agggcgagca gtgccggtaa tgcgttggcc gaccgctacg ccaatgcttt tggtgccgaa
    13741 gtcgaaggcg agtaaggttc cactcatcag gcgtgccctg ccacaccagg catggtgaga
    13801 atatccacac caatcagttt tgccgcctcg cgccagcggt cggcaatcgg cgttttgaac
    13861 agaatattca gatccgccga ggccgttagc cacgcgttat cgagaatttc ttgttccagt
    13921 tgaccttttt cccaggaggc atagcccaga gccaccaata cgtcagacgg ttgtttatcg
    13981 gtgccgagcg tttccagcac atcgcgggaa gtggtcatca ccgtgttgtc tgaaatgcga
    14041 atgctggaag caaaattgga gggcggagta tgcaaaataa acccgcgatc ttcagccagt
    14101 gggccgccga gcataacagg tttatccaga cggattgatt catcacgcgg ctccggcgtg
    14161 atcttcagct tttccagaat cccttcaatt ttgagatttt ccagcggctt gttgacgatg
    14221 atccccattg caccattggt attatgttcg caaatgtaga ccacggaacg acggaaaatc
    14281 ggatcctgga gagcaggcat ggcaataaga aagtgatgct gtaaattcat tgtcagaggt
    14341 tctgtttcct ggttcaaaaa agcaacagcg cccagtatgc gtggaaagaa gggcgctgtc
    14401 actcagagtc tcaacgagat ccttctcgct aaggtgggtt actgctgctg taaacgtgct
    14461 tcgatggcat ccattaacat tccggtgatc gacaccggaa actctgcttc aatctcacga
    14521 atacaggttg ggctggtgac gttaatttca gtcagacggt cgccgatgat atccagacca
    14581 acaaaaatca gccctttttc tttcagcgtc ggcccgatct gacgggcgat tttccagtca
    14641 ctttccgtca gcggccgagg ttcaccgcga ccaccggcag ccagattgcc acgggtttcg
    14701 cccccctgcg gaatacgcgc caggcagtac ggtaccggct cgccatccac caccagcacg
    14761 cgtttgtcgc catctttaat ggctggcagg taattttgcg ccatgcagta gcgagtgcca
    14821 tgctcagtca gggtttcggc aatcacgccg aggtttggat cgccttcttt cacgcggaaa
    14881 atcgacgcgc cgcccatacc gtccagcggc ttaagaatga tgtcgctgtg tttctcccag
    14941 aacgctttta gctgcgcttt attgcgcgta accagcgttt ctggcgttaa gtcagagaac
    15001 caggcggtaa acagtttctc gttacagtcg cgcaggctct gcggcttgtt aacgatcagc
    15061 gtccctttct cttcggcacg ttccagaata taggtcgcgt agataaactc ggtatcaaac
    15121 ggcgggtctt tacgcatcag gagcacatcg agatcggcca gcggcagatc ctgttcaccg
    15181 acgaacgaaa accactcttc gtagttctgc ttcacgttca gcgtgcgggt atgggcgcga
    15241 gcttcgccat tgatcagata cagatcgccc atctccatat agtgaagttc gtaaccacga
    15301 cgctgtgctt ccagcaacat agcgaaactg gaatctttct tgatgttgat gtttgcgatg
    15361 gggtccatca cgatgccgag cttgatcatt attcttctcc gttagcccaa atcgccaaat
    15421 cgtacttgta gcgcggtaat ggcggtgagc gcagttgtct ctgtacgcaa aacgcgaggt
    15481 cccaacagga tatcagtaaa ttgatagcgg gcagtcatgg caatttcatc tgccgataaa
    15541 ccgccttccg ggccaatcag caggcggacg cgttcaaccg gtaacggcaa cgtattgatg
    15601 ctgttactgg cgcgcgggtg aagattcagt ttcagtcctt catcctgctc tgcacaccag
    15661 gcttccagat ccatcgctgg acggatttcc ggcacccggt tacgaccaca ctgctcacag
    15721 gcagcaattg caatcttctg ccactgctgg agcttcttgt tcagacgttc actatccagt
    15781 ttaacgccgc agcgctcaga aaaaagtggc gtaatgaggc ttacaccgag ttcgatcgat
    15841 ttctggatcg taaattccat tttttcacca cgcgacatca cctgaccgag gtgaatatgc
    15901 agcggagatt cgcgatcgtc gatcttgcct tccagcacct tcacttccac gctttttttg
    15961 ctggcgctgg taatttcggc gtcaaagacc tggttgctac cgtcaaacaa ttgcaacgcc
    16021 tgccccggcc ccatgcgcag tacgcgcccg atatggttgg cggcatcttc gcaaagcgcg
    16081 atgtgagaat ggctggtcag tggttcagga tgataaatgc gggggatacg catagttaaa
    16141 aatccgcgtc gtctccccac gcggttgtac gcgtggggta ggggttaaca aaaagagtcc
    16201 cgctagtgta ggttagctct ttcgcgcctg gcaagcgcgt tgcacatacg ggttatgatt
    16261 gccttgcacc ttcgcgatgc gttcatcgcg ctcgcactcc cagtcggtaa ccggatacat
    16321 cttgttccat gcgttgaaca gctgcgtttg ctggcgagag agtgtcaggt tgtattggtc
    16381 gcgcatatag aagtaggtgc gcgcaatggc accgcgtgca cgcgctggtg gttcggcagc
    16441 tttttctttg aaatcgacct tcatggcgca ttgaccgtac tggccttcgc cgccattcca
    16501 ctggctgtac ataaagttgc cgcgatcgcc attcacctca ccgactgacg gctgcaggtt
    16561 atgcatatcg ctttccatct tgcgatagac cggatcttta gcgcagtttt tacgtccacc
    16621 gtcctgccag cactggcgct ggtgaccgaa ctgccaggcg ggaacgacgt gttcccactc
    16681 tacgcggctg gctcggtttt catttttgcg cacctgatag ccgcacgatt gcagatcaac
    16741 aacgcctttt ttgccctgcc agttaatttt acatccgcaa taaaacgtac cgggcgcgtc
    16801 agcgtggact tttaccgccg cggctttcgc ctgagaaaaa ctattgatac cttcggccaa
    16861 cgccgggccg gaaaatgctg cgctcagtac caccgcagca atagacaaat aacggtacat
    16921 cacttactcc gtgttaaaac gagccagcaa cgtagcgaaa gttgttttgg tatgcaatca
    16981 gccagatcag gaaagttcct gatagttcag atggttattt tgcgaccagt tgttcgccgc
    17041 agtgaacaca gcgatagacg gcttcgccac gaactacgcg attatggcgg cgtacggtaa
    17101 gctgatgctc ctggcacttg cagcggtagg ggaaggtgtt gcgacgcacg gattgcagtt
    17161 cgaactgatg cgtacgacgg gcgggaacac ccagcacgtt ttccatcatc cacttccact
    17221 ctttgccatg tggtgctacg cgcccgaaat gtttccatac cagcaaatgt gccagttcgt
    17281 gcggcaccac ttcttcaata aaggcttcac tgttttccaa cagcaaaacg ggattgaggc
    17341 ggatttcata gctttccagc caggctgttc cggcggaggt gccgcgctgg gtataagaga
    17401 gttttggctc cgggtagtta cgccccaatt tcaggttagc ctgggcgagt ttttcccgca
    17461 ggcgacgcat aacggcctgt tggatggcga tagggagacg ggatgttttc atagcggcag
    17521 aggatagagc gaagaaaaga tgactgcaag aggtggcttc ctccgcgatg ggaggaagct
    17581 tggggagatt aatcgtgagc gcctatttcg cgcagtttac gacctttcat cagattacgt
    17641 tcaatatgtt ccagcgaaac gtgtttggtt tccggtacca gccacaatgt cagcaggata
    17701 aacagtacgt tcagagccgc atacacccag aaggtgttgg cgttacccag tgtgttgagc
    17761 atggtcagga acgttgcgcc aacgatcatg ttggcaatcc agttggtggc ggtagagcag
    17821 gtgatgccaa aatcgcggcc tttcagcggc tgaatttcgg agcacagtac ccaaatcagc
    17881 ggaccggcac tcatggcaaa accgacaata aacatcagca gcatggcgat ggcgaaatac
    17941 tgcgctgacg gagagtgaat accgatatgc atcattgtac cgagtacgcc catgccagca
    18001 gccatcacca ggaagcccag cgttagcgtt ggtttacgtc cccagcggtc aacaaggccg
    18061 attgcgataa aggtggcaag tacgttggtc aggccgacaa tcactgtccc ccacatttgc
    18121 tcggtagtgt tggtataacc cgccagttcg aagattttcg gcgcgtaata catgatgacg
    18181 ttcatcccgg tgaattgctg cattacctgt aacagtacgc caaggaacac cgcgcggcgg
    18241 aagttgctat tctctttaaa cagcgcccag ccactctgtt taacctgcaa actttcacgg
    18301 atttcatcca gttcgcgttt cgcttccgcg ctggtgtcac gcaggcgtag cagcacgcgt
    18361 tcggcatcaa caaaacggcg tttggcggca aaccaacgtg ggctgtctgg caggaagaag
    18421 acaccaatca gcagcaaaat tgccgggatg ataatcacac ccagcatcca gcgccatgca
    18481 ccggtgtagc tgaaggcggt atcagaaaga taagcaccga ggatcccgat agtgatcatc
    18541 aactgataca tcgagatcat actgccacga attttttccg gcgcaatttc agagaggtac
    18601 agcggtgcgg tataagaggc cacacccacc gccagcccca gtagaacgcg ggaaagaatc
    18661 agtacttcaa cgtttggcgc agccgcagag aacagcgaac cggcaacaaa caaaattgcg
    18721 ccgatcatca ggctcttttt gcgcccgagt ttaaaggaga gccagccgct gcccaccgca
    18781 ccgactgccg caccgaacat catggagctt acgacccatt cttgcgtgtg cgaagtaatc
    18841 tggaattcat ctgcaataaa cggcagtgcg ccagcaatta caccgatatc caggccaaag
    18901 agtaatcccg ccagagcggc aaggaagcag acgaaaaacg tcattgcctt gtttgaccgc
    18961 ccctgttttt tagcgtcagg catgatgccc tccaatatgg ttatttttta ttgtgaatta
    19021 agataggtga gtacgacgta aaaagatgtg aagcaaatca catcagtgta atcgcttaca
    19081 ctaacattta tcttagttat ggtttaagtg catgaaaata attgagttat atcatcatag
    19141 attaaacgct gttatctgca attaagactt tactgaaaag aaatgtaaca actgtgaaaa
    19201 ctgaataata atatgaaaat attacaaata tttggctggt gttattggtg taatcggttt
    19261 cagtagtgat gataatttgc gataaaacgt gttgaggaag aatgatgtta ttgtgcgggc
    19321 agctttttgc cggatgcggc gtgaatgcct atccaccgcg gtgaggtaaa agaaaaaggc
    19381 cagcgcgagg ctggcctttg aaagcaggtg aagaaagatt acttcagacc ggcagcatcg
    19441 cgcagcagct gcgctttgtc ggttttttcc cacgggaaat gttcacgacc aaagtgaccg
    19501 tatgctgcgg tttctttgta gatcgggtgc agcagatcca gcatctgaat cagaccgtat
    19561 gggcgcaggt cgaagaactc acgtaccagc agagtcagtt gttcagaagg cactttctca
    19621 gtaccgaaag tttctaccat gatggaggtc ggttcagcca cgccgattgc gtaggaaacc
    19681 tgaatttcac aacgatcggc caggccagca gcaacgatgt ttttcgcgac ataacgtgct
    19741 gcgtaggctg cggaacggtc cacttttgat ggatctttac cagagaatgc tccgccaccg
    19801 tgacgtgcca tgccgccgta ggtatcaacg ataattttac gaccagtcag accgcagtca
    19861 cccatcgggc caccgataac aaaacgaccg gtcgggttga tgaagaatgt ggtggcagaa
    19921 gtcagccatt cagcgggcag aattggcttg atgatctctt ccattaccgc ttcttgcagc
    19981 gatttctggt cgatctcttc agagtgctga gtggaaagca cgacagcatc aataccaacg
    20041 attttgccgt cgtcatactg gaaagtcacc tggcttttcg cgtccgggcg cagccacggc
    20101 agagtgccgt ttttacgcac ttcagcctga cgctgtacca gacggtgtgc ataggtgata
    20161 ggtgctggca tcagcacgtc ggtttcatta gttgcgtagc caaacatcag accctggtca
    20221 cccgcgccct gttccagcgg atcggcacgg tcaacgccct ggttgatgtc aggagactgt
    20281 ttgccgatag cgctcagaac cgcacaggag ttagcgtcaa agcccatgtc ggaatgcaca
    20341 tagccaattt cgcgaacggt gttacgggtg atctcttcga tgtctaccca ggcgctggtg
    20401 gtgatttcgc cgccaactaa aaccatgccg gtttttacgt aggtttcgca agcaacgcgt
    20461 gctttcggat cctgttcgag gatcgcgtct aaaacggcat cagagatttg gtcagcaatt
    20521 ttgtcaggat gcccttcaga gacggactcg gacgtaaaaa ggtgttttgc catatttaat
    20581 atcacctaaa gagaatttgg ttagctcaaa ctgttgtgtg gattttctgt ggtagcggat
    20641 cctaccacga ctctgcaggt taaaaacact ggcagtctga gtgttaatcg gtatggatgg
    20701 attaacatct ggatggctat tttaggtcaa ttcttcaccc gatttccact tttttttgaa
    20761 tcgtgtctca ttctgttaaa aacgtggctg gaaatttttt ctgacaatgc cggcattctg
    20821 cgtatttata ttttgcaatt ttctgccatt gtggggtata aaacgcggcg cgcggcttaa
    20881 ataaaaagca cacgacgttt ctttcgtgtt gccacttcca gccgggttga aatcagagtt
    20941 ttggcttgtg ggttcgtctt aacaggcggc cgtggaggtg atacgaaata atgaaccgta
    21001 gtctgctgct taacctgtct caccgttctg gtgaagattc gttccccgca ctctgcatct
    21061 ctgctttgca tacctgccga tgttataccc atctcggcgc ttctcaggat tcaagagctg
    21121 gttacagtta ctgaggactg aacaagggcg ctcttgtaaa aacaagagtt ttctcgtggt
    21181 ttcgccgaac tgtcacactt acgttcggtt atgtgcttaa taatgttatg aaaaagaaac
    21241 cggttgcgca gttggagcgt cagcattcac tgctggaaaa tccatgtgct tatgggttgt
    21301 tatcgcagtt ccaggctgcg atagtcgtta actgttttac acttaataaa ataatttgag
    21361 gttcgctatg tctgacgaca tgtctatggg tttgccttcg tcagcgggcg aacacggtgt
    21421 actacgctcc atgcaggagg ttgcaatgag ctcccaggaa gccagcaaga tgctgcgtac
    21481 ttacaatatt gcctggtggg gcaataacta ctatgacgtt aacgagctgg gccacatcag
    21541 cgtgtgcccg gacccggacg tcccggaagc tcgcgtcgat ctcgcgcagt tagtgaaaac
    21601 tcgtgaagca cagggtcagc gtctgcctgc actgttctgt ttcccacaga tcctgcagca
    21661 ccgtttgcgt tccattaacg ccgcgttcaa acgtgcgagg gaatcctacg gctataacgg
    21721 cgattacttc cttgtttatc cgatcaaagt taaccagcac cgccgcgtga ttgagtccct
    21781 gattcattcg ggcgaaccgc tgggtctgga agccggttcc aaagccgagt tgatggcagt
    21841 gctggcacat gctggcatga cccgtagcgt catcgtctgc aacggttata aagaccgcga
    21901 atatatccgc ctggcattaa ttggcgagaa gatggggcac aaggtctatc tggtcattga
    21961 gaagatgtca gaaatcgcca ttgtgctgga tgaagcagaa cgtctgaatg tcgttcctcg
    22021 tctgggcgtg cgtgcacgtc tggcttcgca gggctcgggt aaatggcagt cttccggcgg
    22081 ggaaaaatcg aagttcggcc tggctgcgac tcaggtactg caactggttg aaacgctgcg
    22141 tgaagctggg cgtctcgaca gcctgcaact actgcacttc cacctcggtt cgcagatggc
    22201 gaatattcgc gatatcgcca cgggcgttcg tgaatccgcg cgtttctatg tggaactgca
    22261 caagctgggc gtcaatattc agtgcttcga cgtcggcggc ggtctgggcg tggactatga
    22321 aggcacccgt tcgcagtctg actgctccgt aaactacggc ctcaatgaat atgccaacaa
    22381 catcatctgg gcgattggtg atgcgtgtga agaaaacggt ctgccgcatc cgacggtaat
    22441 caccgaatcg ggtcgtgcgg tgactgcgca tcacaccgtg ctggtgtcta atatcatcgg
    22501 cgtggaacgt aacgaataca cggtgccgac cgcgcctgca gaagatgcgc cgcgcgcgct
    22561 gcaaagcatg tgggaaacct ggcaggagat gcacgaaccg ggaactcgcc gttctctgcg
    22621 tgaatggtta cacgacagtc agatggatct gcacgacatt catatcggct actcttccgg
    22681 cacctttagc ctgcaagaac gtgcatgggc agaacaactc tatctgagca tgtgccatga
    22741 agtgcagaag caactggatc cgcaaaaccg tgcgcatcgt ccgataatcg acgaactgca
    22801 ggaacgtatg gcggacaaaa tgtacgtcaa tttctcgctg ttccagtcaa tgccggacgc
    22861 atgggggatc gaccagttgt tcccggttct gccgctggaa gggctggatc aagtgccgga
    22921 acgtcgcgct gtgctgctgg atattacctg tgactctgac ggtgctatcg accactatat
    22981 tgatggtgat ggtattgcca cgacaatgcc aatgccggag tacgatccag agaatccgcc
    23041 gatgctcggt ttctttatgg tcggcgcata tcaggagatc ctcggcaaca tgcacaacct
    23101 gttcggtgat accgaagcgg ttgacgtgtt cgtcttccct gacggtagtg tagaagtgga
    23161 actgtctgac gaaggcgata ccgttgcgga tatgctgcaa tatgtgcagc tcgatccgaa
    23221 aacgctgtta actcagttcc gcgatcaagt gaagaaaacc gatcttgatg ctgaactgca
    23281 acaacagttc cttgaagagt tcgaggcagg tttgtacggt tatacttatc tggaagatga
    23341 gtaagtccgt attacttgaa tccgcttaat ttagcggtga taatccgcca caatttattg
    23401 tgacaaatcc aacccttcct cgtcgggcct aacgacgcgg aagggttttt ttatatcgac
    23461 tttgtaatag gagtccatcc atgagcacct taggtcatca atacgataac tcactggttt
    23521 ccaacgcctt tggtttttta cgcctgccga tgaacttcca gccgtatgac agcgatgctg
    23581 actgggtgat tactggtgtg ccgttcgata tggccacttc tggtcgtgcg gggggacgcc
    23641 acggtccggc agcgatccgt caggtttcga cgaatcttgc ctgggaacac aatcgcttcc
    23701 cgtggaattt cgacatgcgt gagcgcctga acgtcgtgga ctgcggcgat ctggtatatg
    23761 cctttggcga tgcccgtgag atgagcgaaa aactgcaggc acacgccgag aagctgctgg
    23821 ctgccggtaa gcgtatgctc tctttcggtg gtgaccactt tgttacgctg ccgctgctgc
    23881 gtgctcatgc gaagcatttc ggcaaaatgg cgctggtaca ctttgacgcc cacaccgata
    23941 cctacgcgaa cggttgtgaa tttgaccacg gcaccatgtt ctataccgcg ccgaaagaag
    24001 gtctgatcga cccgaatcat tccgtgcaga ttggtattcg taccgagttt gataaagaca
    24061 acggttttac cgtgctggac gcctgccagg tgaacgatcg cagcgtggat gacgttatcg
    24121 cccaagtgaa acagattgtg ggtgatatgc cggtttacct gacctttgat atcgactgcc
    24181 tagatcctgc tttcgcgccg ggaaccggta cgccagtgat tggcggcctg acctccgatc
    24241 gcgccattaa actggtacgc ggcctgaaag atctcaacat tgttgggatg gacgtagtgg
    24301 aagtggctcc ggcatacgat cagtcggaaa tcaccgctct ggctgcggcg acgctggcgc
    24361 tggagatgct gtatattcag gcggcgaaaa agggcgagta agcaccagat gcgatgcgca
    24421 cgggtaaaac gtgccattaa tgtcggatgc ggcgtgaacg ccttatccga cctacgttcg
    24481 gcacccgtag gccggataag atgcgccagc atcgcatccg gcaatgcgca caaggtaaca
    24541 aatgtgccat taatgtcaga tgcggcgtga acgccttatc tgacctacgt tcgacaccac
    24601 caggctttac ttaatcccat ccgcgctcat gcgatcgcga atatgctggg cgcgttcggc
    24661 ggatgccgga tggtcgtcaa acattgagct ttggcggcct tcttccagtt tcgccagttt
    24721 ttcaaagctg gtggcaagac ctgccgggct gatgccgcgt tggcgcagaa gatcgtaaga
    24781 ataatcatcg gcttctgctt cctggcgctg ggagaattgc gaattgacta atttctcgcc
    24841 cagatcacca agttgtgatt gagataaact gccgacaatc ccgcccgcag aggccgcagc
    24901 tactcgcacg gcatttgtac caagcgccac ctgcatccct ttcttcacat ggcctaacgc
    24961 cacgtgcccc atctcgtgac cgatcaccgc ttcgacttcg ttatccgtca tcatatccat
    25021 cagcccgcta tagacgcgga tacagccgtt agccattgca aaggcgttca catccttcgc
    25081 catatacact ttgtaattca ccggctgacc gttgatattg ttgcctagcg cattggcaat
    25141 agttgtcaga cgtttagcgt attcgctatt ggctggcgca atcgtcgcct tgctgtccat
    25201 ctcctgacat gcctgatcgc tcagggtttt cacctgcgca tcactcaaac tgtaagcctg
    25261 aaaagcttcc gctcctgatg agagcagtcc gttggagtcc atattctggc aaccagtcag
    25321 taccgttgcc acgctcattg ctaccaataa ggcgcgaatt ttcatgtttt ctcttccgca
    25381 caaggtcagg attattctaa aaatgcgccg tttgcaggtg aatcgacgct cagtctcagt
    25441 ataaggaaaa gcgcagcgga cgggcgagta gattgcgcaa catgcgagca tgatccagag
    25501 atttctgaag cggcaaaagg atgttccatg tacatgacgc gcggcttgcg gtaaattgtt
    25561 ggcaaatttt ccggcgtagc ccaaaacgcg ctgtcgtcaa gtcgttaagg gcgtgccctt
    25621 catcatccga tctggagtca aaatgtcctc acgtaaagag cttgccaatg ctattcgtgc
    25681 gctgagcatg gacgcagtac agaaagccaa atccggtcac ccgggtgccc ctatgggtat
    25741 ggctgacatt gccgaagtcc tgtggcgtga tttcctgaaa cacaacccgc agaatccgtc
    25801 ctgggctgac cgtgaccgct tcgtgctgtc caacggccac ggctccatgc tgatctacag
    25861 cctgctgcac ctcaccggtt acgatctgcc gatggaagaa ctgaaaaact tccgtcagct
    25921 gcactctaaa actccgggcc acccggaagt gggttacacc gctggtgtgg aaaccaccac
    25981 cggtccgctg ggtcagggta ttgccaacgc agtcggtatg gcgattgcag aaaaaacgct
    26041 ggcggcgcag tttaaccgtc cgggccacga cattgtcgac cactacacct acgccttcat
    26101 gggcgacggc tgcatgatgg aaggcatctc ccacgaagtt tgctctctgg cgggtacgct
    26161 gaagctgggt aaactgattg cgttctacga tgacaacggt atctctattg atggtcacgt
    26221 tgaaggctgg ttcaccgacg acaccgcaat gcgtttcgaa gcttacggct ggcacgttat
    26281 tcgcgacatc gacggtcatg acgcggcatc catcaaacgc gcagtagaag aagcgcgcgc
    26341 agtgactgac aaaccgtccc tgctgatgtg caaaaccatc atcggtttcg gttccccgaa
    26401 caaagccggt actcacgact cccacggtgc gccgctgggc gacgctgaaa ttgccctgac
    26461 ccgcgaacag ctgggctgga aatatgcacc gttcgaaatc ccgtctgaaa tctatgctca
    26521 gtgggatgcg aaagaagcag gccaggcgaa agaatccgca tggaacgaga aattcgctgc
    26581 ttacgcgaaa gcttatccgc aggaagccgc tgaatttacc cgccgtatga aaggcgaaat
    26641 gccgtctgac ttcgacgcga aagcaaaaga gttcattgct aaactgcagg ctaacccggc
    26701 gaaaatcgcc agccgtaaag cgtctcagaa tgctatcgaa gctttcggtc cgctgttgcc
    26761 ggaattcctc ggcggttctg ctgacctggc accgtctaac ctgaccctgt ggtctggttc
    26821 taaagcaatc aacgaagatg ctgcgggtaa ctacatccac tacggtgttc gcgagttcgg
    26881 tatgaccgcg attgctaacg gtatctccct gcacggtggt ttcctgccgt acacctccac
    26941 cttcctgatg ttcgtggaat acgcacgtaa cgccgtacgt atggctgcgc tgatgaaaca
    27001 gcgtcaggtg atggtttaca cccacgactc catcggtctg ggcgaagatg gcccgactca
    27061 ccagccggta gagcaggtcg cttctctgcg cgtaaccccg aacatgtcta catggcgtcc
    27121 gtgtgaccag gttgaatccg cggtcgcgtg gaaatacggt gttgagcgtc aggacggccc
    27181 gaccgcactg atcctctccc gtcagaacct ggcgcagcag gaacgaactg aagagcaact
    27241 ggcaaacatc gcgcgcggtg gttatgtgct gaaagactgc gccggtcagc cggaactgat
    27301 tttcatcgct accggttcag aagttgaact ggctgttgct gcctacgaaa aactgactgc
    27361 cgaaggcgtg aaagcgcgcg tggtgtccat gccgtctacc gacgcatttg acaagcagga
    27421 tgctgcttac cgtgaatccg tactgccgaa agcggttact gcacgcgttg cggtagaagc
    27481 gggtattgct gactactggt acaagtatgt tggcctgaat ggcgctatca tcggtatgac
    27541 caccttcggt gagtcagctc cggcagagtt gctattcgaa gagttcggct tcaccgtcga
    27601 caacgtcgtt gcgaaagcaa aagaactgct gtaattagca tttcgggtaa aaaggtcgct
    27661 tcggcgacct tttttattac cttgatatgt ccgtttgcgg acaagcaata gataaagcgt
    27721 gttgcagatc acaaatattt atatgcaata aatatcaatt atgtaatatg catcacgata
    27781 tgcgtattga catttgttgt tatatctata actcaatgtt atataagaaa ttaataattc
    27841 actgttttca aaacaccggt ttccctgctc aattgctttc attaaaccgc acgagtattt
    27901 tatttttacc gccggacggt accctaaaaa taaaataagg ataatttatg cggcttagtg
    27961 actattttcc agaatcatca atctcagtta tacattcagc aaaagattgg caggaagcta
    28021 tcgatttctc gatggtatca ttgctggata aaaactatat cagcgagaat tacattcagg
    28081 caattaaaga ttccaccatt aacaatggcc cttattatat tctcgcacca ggcgtggcaa
    28141 tgcctcatgc gcgaccggaa tgtggggcgc ttaaaaccgg gatgtctttg acattacttg
    28201 aacaaggtgt ttattttccg gggaatgacg aaccaattaa attactcatc ggactctctg
    28261 ctgccgatgc cgattcgcac attggcgcta ttcaggcgtt aagtgaatta ctgtgcgaag
    28321 aagaaatact cgaacaactc ttaacagcat catcagaaaa acaattagcg gacattatca
    28381 gccacggata attactctct cctctacagg ataatattat ggaaaacaag tctgctcgtg
    28441 caaaggtcca ggcttttggg ggctttttga ctgcaatggt catccccaat attggtgctt
    28501 ttattgcctg gggttttatt actgcgttat ttattcccac cggttggctg cctaacgaac
    28561 atttcgccaa aattgtcggc ccgatgatta cctatttatt gcccgtgatg attggttcta
    28621 caggtggtca tctggtcggc ggtaaacgcg gggcggtcat gggcggaata ggtactattg
    28681 gtgtgatcgt tggcgcagag atcccgatgt tccttggctc aatgatcatg gggccgctcg
    28741 gtgggttggt cataaaatat gtcgataagg cactggaaaa acgcatacct gccggttttg
    28801 agatggttat caataacttc tcattaggta tcgcggggat gctcctttgt ctgctgggtt
    28861 ttgaagtgat cggcccggcg gtgttaattg ccaatacttt cgtcaaagag tgtatcgagg
    28921 cgctggtaca tgcgggttat ctgcctctgc tgtcagtcat caatgaaccg gcgaaagtgc
    28981 ttttccttaa taatgcgatc gatcagggcg tctattatcc gctgggaatg caacaggctt
    29041 cggttaacgg taaatccatc ttctttatgg tggcctctaa cccaggtccg ggcctggggc
    29101 tgctgctggc gtttaccttg tttggtaaag ggatgagtaa acgttctgcg cccggggcga
    29161 tgattattca cttcctcggt gggatccacg aactgtattt cccatatgtg ctgatgaagc
    29221 cgctgactat tattgccatg attgcgggcg gtatgtctgg tacctggatg tttaacttac
    29281 tggacggtgg tctggtggct ggcccaagtc tggggtctat ctttgcttac ctggcactga
    29341 cgccgaaagg ctcgttcctg gcgacaattg ccggtgttac ggtaggtacc ctggtgtcct
    29401 ttgctattac ttcgctgata ctgaagatgg aaaaaacggt ggaaacggag agcgaagatg
    29461 agtttgctca gtcagccaat gcggttaagg cgatgaaaca agagggtgca ttctcgttaa
    29521 gcagggttaa gcgtatcgcc tttgtttgcg atacggggat gggctccagt gcgatgggcg
    29581 cgaccacctt ccgtaaacgc ctggaaaaag cggggctggc aatagaagta aaacattacg
    29641 cgatagaaaa cgtgcctgcg gatgccgata tcgtcgtcac tcacgccagt ctggaagggc
    29701 gcgtgaaacg tgtgacggat aaaccgctga tattgattaa taactatatt ggcgatccaa
    29761 aactcgacac tttatttaat caattaaccg ccgaacataa acactgatta cagaggtaaa
    29821 aatgaaaacc aaagttgctg ctatttatgg caagcgggat gtccgtctgc gcgaatttga
    29881 actgccagaa attaccgata atgaactgtt agtgagtgta atttctgaca gcgtctgttt
    29941 atcgacctgg aaagcggcgt tactcggtag tgaacataaa cgcgtacccg acgatttaga
    30001 aaatcatccg gtcattaccg ggcatgaatg tgccggggtt attgtcgaag tgggtaaaaa
    30061 tctcactggc aaatataaaa aaggccagcg ttttgtattg caaccggcga tggggttacc
    30121 aagcggatat tcagcaggct acagctacga atattttggc ggtaacgcca cctatatgat
    30181 tattcccgaa atcgccatta atttgggctg cgtattaccg tatcacggct cttattttgc
    30241 tgcggcgtcg ctggtagagc ctatgtgctg cattattggt gcttatcatg ccaattatca
    30301 caccacgcaa tatgtttatg agcatcgcat gggcgtcaaa cctggcggca atattgcact
    30361 gctggcgtgt gcgggtccga tgggcattgg cgctatcgat tacgccatta acggtggcat
    30421 acaaccgtca cgggtggtgg tggtcgatat cgacgacaaa cgcctggcgc aggtgcagaa
    30481 gctgctgccg gtggatctgg cggccagtaa aggcattgag ctggtgtatg tgaataccaa
    30541 agggatgagc gatccggtcc agacgctgcg ggcgctgaca ggagatgtcg ggttcgatga
    30601 catttttgtt tatgcggcgg tgcctgctgt cgttgagatg gctgacgaat tactggcgga
    30661 agatggctgt ctgaacttct ttgccgggcc gacggataaa aacttcaaag tgccgtttaa
    30721 tttctacaac gtccattaca acagcacgca cgtagtcggt acatccggcg gttcaacgga
    30781 cgacatgaaa gaggcgattg cgttaagcgc cacggggcag ttgcagccgt cctttatggt
    30841 cacgcatatc ggtgggctgg atgcggtgcc agataccgtg ctcaatctgc cggatatccc
    30901 tggcggtaaa aaactcattt ataacggcgt gaccatgccg ctcactgcca ttgccgattt
    30961 tgccgaaaaa ggcaaaaccg atccgctatt taaagagttg gcgcggctgg ttgaggaaac
    31021 gcacggcatc tggaatgaac aggccgagaa atatctgctg gcacaatttg gcattgatat
    31081 cggggaggcc gcgcaatgat gtccctggcg tggccattat ttcgcgttac ggaacaggca
    31141 gcgttggctg cctggccgca aaccggatgt ggcgacaaaa ataaaattga tggcctggcg
    31201 gtcaccgcga tgcgccaggc attaaacgac gtcgcttttc gtggacgagt ggttatcggc
    31261 gaaggagaaa ttgaccatgc gccgatgctg tggattggcg aagaggtggg caaaggcgat
    31321 gggccagaag tcgatatcgc ggttgatccc attgaaggca cgcggatggt ggcgatgggg
    31381 cagagcaatg cgctggcggt catggctttc gcccctcggg atagcctgtt acatgcgccc
    31441 gatatgtata tgaaaaagct ggttgttaat cgactggctg ccggggtgat tgatctgtct
    31501 ttgcctctgg cggacaactt gcgtaacgtg gcgcgggcgt taggtaaacc gctggacaag
    31561 ctgcgcatgg ttacgctgga taaaccgcgc ctgagtgccg cgattgagga agcaacacag
    31621 ctgggcgtga aggtttttgc cctgccggat ggcgatgtcg ccgccagcgt gctgacctgc
    31681 tggcaggata atccttacga tgtgatgtac accatcggcg gtgcgccaga aggcgtgatt
    31741 tctgcctgtg ccgttaaagc attaggcggg gatatgcagg ccgagctgat tgacttttgt
    31801 caggctaaag gggattacac ggaaaatcgg cagattgcag agcaggagag caagcgttgc
    31861 aaggcaatgg gcgttgacgt taaccgtgtg tactcgctcg atgaactggt aagggggaac
    31921 gatatcctct ttagcgctac gggcgtgacg ggcggcgagt tggtgaacgg tatccaacag
    31981 acggcgaatg gggtgcggac gcagacatta ctgatcggcg gcgcggacca aacgtgtaat
    32041 ataatagact ccctgcattg atggtgatct atcggaagac caatggcgaa gctgacagaa
    32101 gatgatgtgc ttgagcaact ggatgcacag gacaatttat tttcatttat gaaaactgcg
    32161 cattctattt tgctccaggg gatacgccag tttctgccgt cgctgtttgt cgataacgat
    32221 gaagagatcg tggaatatgc agtgaagccg ttactcgccc aaagcggccc gcttgacgat
    32281 attgatgttg cgctgcgttt gatttatgcg ctggggaaaa tggataaatg gctgtacgcc
    32341 gatatcacgc atttttccca gttctggcat tacctgaacg aacaggatga aacgcccgga
    32401 tttgccgatg acatgacctg ggattttatc agcaacgtca atagcataac ccgcaatgcg
    32461 atgctctatg acgcgttgaa agcgatgaag ttcgccgatt tcgccgtctg gtcagaggcg
    32521 cgttttagcg ggatggtcaa aacggcgctg acgctggcag taacgacaat cttaaaggaa
    32581 ttaacgccgt gaaaattgaa ttaacggtga atgggctgaa gatacaggca cagtaccaga
    32641 acgaagagat tgaaaatgta cataaaccgt tgctgcgtat gctggcggcc ttacagacgg
    32701 taaatccgca gcggcgtacg gtggtttttc tttgcgctcc gccggggacg ggtaaatcta
    32761 ccctaaccac cttctgggaa tatctcgcgc aacaagaccc ggagctgccc gctattcaga
    32821 cgctcccgat ggatggtttt caccattaca atagctggct ggacgcgcat caattgcgcc
    32881 ccttcaaagg cgcaccagag acatttgacg ttgcgaaact ggcggaaaat ctgcgccagg
    32941 tggtggaagg ggattgtacg tggccgcagt acgatcgaca aaagcatgat cctgttgaag
    33001 atgcgttgca cgttaccgca ccactcgtca tcgtcgaagg aaactggttg ttgctggacg
    33061 acgagaagtg gtgccagctt gcgcaatttt gtgatttctc gatctttatc aacgcgccag
    33121 ctactgcgtt gcgggagcgt ctggtggggc gcaagctggc gggaggggta tcgctggcag
    33181 atgcagaagc cttttatgac cgtaccgacg ggccgaatgt tcgccgggtg ctggaagaga
    33241 gtctgcctgc aaatttgacc ttaatgatga ccgctacggg tgaatatcgt ctggtggatt
    33301 aacgttcctg attacaccat cttccagtcg caccacacga gagaaatgac gccgggtaaa
    33361 tgcggcgtca tgactgattg ctaccacgct cgtgcctcgc tggcggcact tctccagcca
    33421 gctttcaaac acgctcaacc aattttcgtc aaaatcccga ctcggctcgt ccagcagtaa
    33481 tagtggcgga gagagtgctt ccaggcaggc gacggcgacc atccgtcgct gcgcactatg
    33541 cagatccaac gggtgcgagt tggcagtgtc tgctaactga caacattgca gggcagcatg
    33601 ggtacgttga gtgatttcat ccgccgggca tttttgcagt ttcaaaccaa atgcgatctc
    33661 gtcagcgacg gtgctgtgaa aaagttggtt ttccgcttcc tgaaacagta cgccaacctt
    33721 tgccgcgcgc tggcggtttt tcaggttttt catcgcctgt tgttgcagca taaccgtacc
    33781 ggctgtgggc gtaagaagac ctgccattac tcgtaacagc gtcgatttcc ctgcgccatt
    33841 gtcaccggtc agcgccaacc attcaccttg tttgagttgc agagaaatgt cgcaaagaca
    33901 atccgttgcc gcgccaggcc agcgataaga tatctgattt aacgttaaca tagtggctgg
    33961 agcgctccgt tttgcaattg ccacgcttgc tggcagtagt tcaggaaagg tgtgcgatga
    34021 cgttcaaaaa gaataatcag cgaaccacgt tcaaacgccc agtgttgtaa gcgctgcaac
    34081 agcatttcgc tggcctgcgg cgtcaggcgg ctaaaggctt catcaagaat taagagcttt
    34141 ggctgcatcg cgatggcgca ggcgatgacc acgcgctgtg tctctccacc ggagagtgtt
    34201 gccgggtgac gatggcgcaa cggctggcac tcggtcagag tcagggcggc atcaatccgc
    34261 gccataatct ctgcttcagc aagacacaga ttttctggcc caaaggcaac ttcttcttcc
    34321 acgctaaagg tacagccgga gagctgcaaa tagggcgatt gctggacgag ctggatggtg
    34381 gcggattgtt cattaagcgg gagatgccca atgggcgtac cgaggagcgt ccccgtgcct
    34441 gttatctctc ctggcagaaa gtccggatac cagcccgcca tcagctgtgc cagcgtactt
    34501 ttgccactgc cgttgtcacc aaagatggcg accatccccg gtttgacata gtgaaagtca
    34561 taacagaacg gaggatgcgt tgagtgtgtc gggcaataac ggaactgttc taacgtaacc
    34621 atatccacgc tccgaattct gtcagcatca gcaagatcat ggtgtaacgc gctacgcgtt
    34681 gtagggtgct atcggctggt gcccacaacg ttgttcgtcg gttgtggatc cgaaatgccc
    34741 gcatatccag cgccgcacca cggacagcca gatcgttaag cgcattgtgt gttagcggaa
    34801 taattaacgc gggcatcgca cgtaaacgct gataccagcc ctcatccagc ggcacgccgc
    34861 gggcacgctg cgcttcatga ataatcgcta attgccgttt taactgctca acgaccaata
    34921 acggcccggc aaacagataa gcgacgcctg gcggcaggcg agaagcaaac agagcgcgaa
    34981 taaaacattg cacggggacg tactgcatcc acaactgtga ggttgaaaca atcgccagaa
    35041 tccgcaacca gagcgtaata gcatgcgacc accgttctgg cgagcgtggc gtgccgctta
    35101 gccattctgg tagccagcca ccgtgtacca gccacagccc ggctcccagc gagaacatca
    35161 gccagacgac atattttgcc cgccgccgcg tggcctttag cgcaataagg cagaaaaagg
    35221 tcgccgcgct atagatcggc aaaatggtct gcgcgggcag gataagcgtg gtgcaggccg
    35281 ccaatgccca cagtattaat gaggtaaatg gatgcattta gcgcacggca gccattgccg
    35341 gaaaatggcg cgtggtgcga atcggcagct ggcgcagtaa aagccagaca atcaccgctg
    35401 taagaatttt atcgaccagg ttcgcgccta tcaccgtaat cgccaccgac tccaccaggt
    35461 tttgccccat cgagtgcatc caggcaacga acaaatcggc tccgctaccg gtgacaccgc
    35521 cgaacagcgc agtacgcagc ggtacagcga cgaccgttac cgcaagcgta ataatcacgc
    35581 cgctgacaac gacttttggc agggtgcgaa accaacccgc tcgcgccagc caaccggcga
    35641 ccaggccgat gaccatcgcg acgggtgcaa atgcggcggc gatcgggtcg gtcagcaatc
    35701 cccagagtaa attggtcagt aagccggtca gcattccgat caccggacca agcagtaccg
    35761 cgctaataag tgtgccgatg gagtcgagaa atataggtaa tttgaccatg ctggccagtt
    35821 gcccgccgat catattgatg gcgatagata taacgatcaa caccagtgcc tgactggaaa
    35881 agtggctacg cgccataaga aaacctcttt gaaagtagtt atttaacggg ggaaatatgc
    35941 aactcgtcgt aaccagaaag cgcgcaaggt tggcggctaa acgccagttc ctcaaggcta
    36001 ccgatcacca gttcaagcgt ggtccgcacg gtacagccgg gcatcatatg accgccaagc
    36061 atcgtgccgt gtggatcgga aacgcagaga tgaagatgct caccactctg ttccagcgtg
    36121 ccgtttagtg agatcacttc gaatgtaccg ttcaggagcg tggtgccttc ttgcctggca
    36181 tagcgcaagg cgacgtcagt caggctaccg gtacagcctg caatccaggc ggcatgaagt
    36241 tgatgctgct gtgcaaaagc gtgtagctgg gaaaaaactt cctgcccggg gaggaggcgc
    36301 agtgcgtaaa agcgtgcgga ggaagaatat gggtagggca atgtcatcat tggagtactc
    36361 cgaaactcgg acgtattgcg gataatatcg acgctgcatc agtcatattc tgatataagt
    36421 cagggtttgt gacggcacaa cgaaaagatt ttgctacgct tattcgtgat tctgcaaagt
    36481 ggaataaatt ctacaggggc ggggctgaaa acgtgacgta agtcagataa ttgctttctt
    36541 tgactggaca atcattcctt ttattccacg tttcgcttat cctagctgaa gcgtttcagt
    36601 cgattaaatg ttcgacaatt aaccaatcag tcgcagtttg cgacaggtaa ggtttccccg
    36661 gacgatttgc tggattactc tgtctggcaa atttgctcag ggaaaacctt gcaggagatc
    36721 tatgaccgta cgcgtagcga taaatggctt cggtcgcatc gggcgtaatg tggttcgtgc
    36781 tttgtatgaa tccggacgcc gggcggaaat taccgtggtg gcaatcaacg aactggcgga
    36841 tgctgcgggc atggcgcatt tgttgaaata tgacaccagc catggccgtt ttgcatggga
    36901 agtacgacag gaacgcgatc aactttttgt tggtgatgac gccatccgcg tattgcatga
    36961 acgttcactg caatcgctcc cctggcgtga acttggcgtt gatgtagtcc tcgactgcac
    37021 cggcgtatat ggctcccgcg agcatggcga agcgcatatt gccgccgggg ccaaaaaagt
    37081 gctcttttca catcctggca gtaacgatct cgacgcgacc gttgtttacg gcgtcaatca
    37141 ggatcaactt cgtgcggaac accgcatcgt ttctaacgct tcctgtacca cgaattgcat
    37201 aattcccgtc atcaaattgt tagatgatgc gtacggtatt gagtccggca ctgtgaccac
    37261 aattcactcc gccatgcacg atcaacaggt tattgatgca taccatcctg acctgcgtcg
    37321 cacccgggca gccagccagt cgatcattcc ggtcgatact aaactggccg ccggtatcac
    37381 acgatttttt ccgcaattta acgatcgctt tgaagcgatt gcggtacgtg tgccaaccat
    37441 aaatgtgacg gcaatcgatt taagcgtgac ggtgaagaaa cctgtaaaag ccaatgaagt
    37501 caacctgttg ctgcaaaaag cagcacaagg tgcatttcat ggtatagttg actatacgga
    37561 attgccgttg gtctctgtag attttaacca cgatccgcac agtgccattg tcgatggcac
    37621 ccaaacccgg gtcagtggcg cacacctgat caaaacgttg gtctggtgcg ataacgaatg
    37681 gggctttgct aaccgaatgc tcgacacgac gttagctatg gctactgttg ctttcaggta
    37741 agacgcaagc agcgtctgca aaacttttag aatcaacgag aggattcacc atgtctgtaa
    37801 ttaagatgac cgatctggat cttgctggga aacgtgtatt tatccgtgcg gatctgaacg
    37861 taccagtaaa agacgggaaa gtaaccagcg acgcgcgtat ccgtgcttct ctgccgacca
    37921 tcgaactggc cctgaaacaa ggcgcaaaag tgatggtaac ttcccacctg ggtcgtccta
    37981 ccgaaggcga gtacaacgaa gaattctctc tgctgccggt tgttaactac ctgaaagaca
    38041 aactgtctaa cccggttcgt ctggttaaag attacctcga cggcgttgac gttgctgaag
    38101 gtgaactggt tgttctggaa aacgttcgct tcaacaaagg cgagaagaaa gacgacgaaa
    38161 ccctgtccaa aaaatacgct gcactgtgtg acgtgttcgt aatggacgca ttcggtactg
    38221 ctcaccgcgc gcaggcttct actcacggta tcggtaaatt cgctgacgtt gcgtgcgcag
    38281 gcccgctgct ggcagctgaa ctggacgcgc tgggtaaagc actgaaagaa cctgctcgcc
    38341 cgatggtggc tatcgttggt ggttctaaag tatctaccaa actgaccgtt ttggactccc
    38401 tgtctaaaat cgctgaccag ctgattgttg gtggtggtat cgctaacacc tttatcgcgg
    38461 cacaaggcca cgatgtgggt aaatccctgt acgaagctga cctggttgac gaagctaaac
    38521 gtctgctgac cacctgcaac atcccggttc cgtctgatgt tcgcgtagca accgagttct
    38581 ctgaaactgc accggctacc ctgaaatctg ttaacgatgt gaaagctgac gagcagatcc
    38641 tggatatcgg tgatgcttcc gctcaggaac tggctgaaat cctgaagaat gcgaaaacca
    38701 ttctgtggaa cggtccggtt ggcgtgttcg aattcccgaa cttccgcaaa ggtactgaaa
    38761 tcgtggctaa cgctatcgca gacagcgaag cgttctccat cgctggcggc ggcgacactc
    38821 tggcagcaat cgacctgttc ggcattgctg acaaaatctc ctacatctcc actggcggcg
    38881 gcgcattcct cgaattcgtg gaaggtaaag tactgcctgc agtagcgatg ctcgaagagc
    38941 gcgctaagaa gtaaaaaatc acagggcagg gaaacctgcc cttgtttcag cgcgctttta
    39001 gagcacgcat tttttcatac tctaaataat tcgagttgca ggaaggcgac aagagagtga
    39061 atccccagga gcttacataa gtaagtgact ggggtgagcg aacgcagacg cagcacatgc
    39121 aacttgaagt atgatgagta taaggcccga cgatacagga caagagacat gtctaagatt
    39181 tttgatttcg taaaacctgg cgtaatcact ggtgatgacg tacagaaagt tttccaggta
    39241 gcaaaagaaa acaacttcgc actgccagca gtaaactgcg tcggtactga ctccatcaac
    39301 gccgtactgg aaactgctgc taaagttaaa gcgccggtta tcgttcagtt ctccaacggt
    39361 ggtgcttcct ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg tgctgctatc
    39421 ctgggcgcga tctctggtgc gcatcacgtt caccagatgg ctgaacatta tggtgttccg
    39481 gttatcctgc acactgacca ctgcgcgaag aaactgctgc cgtggatcga cggtctgctg
    39541 gacgcgggtg aaaaacactt cgcagctacc ggtaagccgc tgttctcttc tcacatgatc
    39601 gacctgtctg aagaatctct gcaagagaac atcgaaatct gctctaaata cctggagcgt
    39661 atgtccaaaa tcggcatgac tctggaaatc gaactgggtt gcaccggtgg tgaagaagac
    39721 ggcgtggaca acagccacat ggacgcttct gcactgtaca cccagccgga agacgttgat
    39781 tacgcataca ccgagctgag caaaatcagc ccgcgtttca ccatcgctgc gtccttcggt
    39841 aacgtacacg gtgtttacaa gccgggtaac gtggttctga ctccgaccat cctgcgtgat
    39901 tctcaggaat atgtttctaa gaaacacaac ctgccgcaca acagcctgag cttcgtattc
    39961 cacggtggtt ccggttctac tgctcaggaa atcaaagact ccgtaagcta cggcgtagtg
    40021 aaaatgaaca tcgataccga tacccaatgg gcaacctggg aaggcgttct gaactactac
    40081 aaagcgaacg aagcttatct gcagggtcag ctgggtaacc cgaaaggcga agatcagccg
    40141 aacaagaaat actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc gatgatcgct
    40201 cgtctggaaa aagcattcca ggaactgaac gcgatcgacg ttctgtaaga tattcctttc
    40261 tgcttatctc aaggcccgct ctgcgggtct ttttttcgcc aaaagcaaaa ttgcctgatg
    40321 cgctacgctt atcaggccta cacgtatcct gcaatatatt gaatttgcag gtttttgtag
    40381 gccggataag gcgttcacgc cgcatccggc atgacaaagc gcacattgat atctgtctga
    40441 gccccgctct gcggggattt tttcgccaaa agcaaggtga ttcagatgag aaatctgtga
    40501 cctatttggc aaaattatgc tttattgttt acccttgtca gactgcccgt cataaggcgg
    40561 cggagtgtat ttctccattt tgagtcagtt gaaaaggaat attgaatgga agatttgaat
    40621 gttgtcgata gcataaacgg cgcgggaagc tggctggtag ctaaccaggc gctgctgcta
    40681 agttatgcag taaacatcgt ggcggcactc gcgatcatca tcgttggttt gattatcgcg
    40741 cggatgattt ccaacgcggt gaatcgcctg atgatctccc gtaaaatcga tgccactgtt
    40801 gctgattttc tttctgcatt agtccgttac ggtattatcg cctttacgct aatcgctgcc
    40861 ctgggacgcg tgggtgtaca aacagcgtca gtcattgctg tactcggtgc cgcaggctta
    40921 gctgttggtc tggctttgca ggggtcactt tctaacctgg ccgctggcgt gttacttgtc
    40981 atgttccgac cgttccgtgc cggagaatat gttgacctcg gcggcgtagc cggtactgtg
    41041 ctgagtgtgc agattttctc caccaccatg cgtactgcag acggtaaaat tatcgttatt
    41101 ccgaacggta aaattattgc cggaaatatt attaacttct cccgcgagcc agttcgccgt
    41161 aacgaattta ttattggcgt ggcgtatgat tccgatatcg atcaggttaa gcagatcctg
    41221 accaatatta tccagtctga agatcgcatt ttgaaagatc gcgaaatgac tgtgcgcctg
    41281 aacgaacttg gtgcatcgtc gattaatttc gtggtccgcg tctggagcaa cagcggcgat
    41341 ctgcaaaacg tgtactggga tgtgctggag cgtattaaac gtgaatttga tgccgccggt
    41401 atcagcttcc cgtacccgca aatggatgtg aactttaagc gggtgaaaga agacaaagct
    41461 gcgtaatcaa cgctgaggcc agataatact ctctggcctc tctcttatta gttattctga
    41521 ttgccaatta atattatcaa tttccgctaa taacaatccc gcgatatagt ctctgcatca
    41581 gatacttaat tcggaatatc caacgtgttt tcttattact ttcaaggtct tgatcttggg
    41641 gc
//
LOCUS       DAJTSL010000011        41588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTSL010000011 DAJTSL010000000
VERSION     DAJTSL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.011"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..610)
                     /gene="yeeE"
                     /locus_tag="OO433_000541"
                     /pseudo
     CDS             complement(1..610)
                     /gene="yeeE"
                     /locus_tag="OO433_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
     gene            complement(789..2147)
                     /gene="plaP"
                     /locus_tag="OO433_000542"
     CDS             complement(789..2147)
                     /gene="plaP"
                     /locus_tag="OO433_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6049137.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYRVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="OO433_000543"
     CDS             complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="OO433_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6049138.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(2414..3343)
                     /locus_tag="OO433_000544"
     CDS             complement(2414..3343)
                     /locus_tag="OO433_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6049139.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQHFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(3389..4213)
                     /locus_tag="OO433_000545"
     CDS             complement(3389..4213)
                     /locus_tag="OO433_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6049140.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGLVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="OO433_000546"
                     /pseudo
     CDS             complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="OO433_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
     gene            complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="OO433_000547"
     CDS             complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="OO433_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6049141.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            5081..5131
                     /gene="hisL"
                     /locus_tag="OO433_000548"
     CDS             5081..5131
                     /gene="hisL"
                     /locus_tag="OO433_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6049142.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    5107..5230
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            5277..6176
                     /gene="hisG"
                     /locus_tag="OO433_000549"
     CDS             5277..6176
                     /gene="hisG"
                     /locus_tag="OO433_000549"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6049143.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            6182..7486
                     /gene="hisD"
                     /locus_tag="OO433_000550"
     CDS             6182..7486
                     /gene="hisD"
                     /locus_tag="OO433_000550"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6049144.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSLESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            7483..8553
                     /gene="hisC"
                     /locus_tag="OO433_000551"
     CDS             7483..8553
                     /gene="hisC"
                     /locus_tag="OO433_000551"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6049145.1"
                     /translation="MSIVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYGVSAETIGVECRTVPTLDNWQLDLQGISDKLEGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     ECLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            8553..9620
                     /gene="hisB"
                     /locus_tag="OO433_000552"
     CDS             8553..9620
                     /gene="hisB"
                     /locus_tag="OO433_000552"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6049146.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAIDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            9620..10210
                     /gene="hisH"
                     /locus_tag="OO433_000553"
     CDS             9620..10210
                     /gene="hisH"
                     /locus_tag="OO433_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6049147.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            10210..10947
                     /gene="hisA"
                     /locus_tag="OO433_000554"
     CDS             10210..10947
                     /gene="hisA"
                     /locus_tag="OO433_000554"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6049148.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYATQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10929..11705
                     /gene="hisF"
                     /locus_tag="OO433_000555"
     CDS             10929..11705
                     /gene="hisF"
                     /locus_tag="OO433_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6049149.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEVVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11699..12310
                     /locus_tag="OO433_000556"
     CDS             11699..12310
                     /locus_tag="OO433_000556"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6049150.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVAFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLLLVNPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(12407..13384)
                     /gene="wzzB"
                     /locus_tag="OO433_000557"
     CDS             complement(12407..13384)
                     /gene="wzzB"
                     /locus_tag="OO433_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6049151.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(13531..13623)
                     /locus_tag="OO433_000558"
                     /pseudo
     CDS             complement(13531..13623)
                     /locus_tag="OO433_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            13793..14797
                     /locus_tag="OO433_000559"
     CDS             13793..14797
                     /locus_tag="OO433_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HCR6049152.1"
                     /translation="MKFLVTGAAGFIGFYLSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFERVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKNPQWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            complement(14863..16029)
                     /gene="ugd"
                     /locus_tag="OO433_000560"
     CDS             complement(14863..16029)
                     /gene="ugd"
                     /locus_tag="OO433_000560"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR6049153.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKRQSP
                     LVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSERAKRFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKNGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(16278..17684)
                     /gene="gndA"
                     /locus_tag="OO433_000561"
     CDS             complement(16278..17684)
                     /gene="gndA"
                     /locus_tag="OO433_000561"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6049154.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIT
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFHDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELIAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEEGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDNAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(17789..18622)
                     /locus_tag="OO433_000562"
     CDS             complement(17789..18622)
                     /locus_tag="OO433_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6049155.1"
                     /translation="MNHRLEKFSVLISIYKNDLPQFFEVALRSIFHDQTLKPDQIVIV
                     ADGELHQTHIDIINSFIDDVGNKIVTFVPLPRNVGLANALNEGLKACRNELVARMDAD
                     DISLPHRFEKQISFMINNSEIDVCGSFIDEIETVTEEFISTRKVPLEHREIVKFARKR
                     SAVSHPSVIFRKNTVLAVGGYPPFRKSQDFALWSLLIVHNARFANLPDILLKMRTGRN
                     LMARRGLSYLLYEYKVLYYQYKIGFIRKNELISNAMLRTFFRIMPSKLKELMYSIVRN
                     R"
     gene            complement(18619..19488)
                     /locus_tag="OO433_000563"
     CDS             complement(18619..19488)
                     /locus_tag="OO433_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6049156.1"
                     /translation="MDTVYAVLVAYNPEHNDLKNAVELLLRQVTKVVVCNNSTNGYKY
                     AENSSGDVKIFNFNDNLGIAEAQSIGMKWAFENGADFILQMDQDSIPDPKMVEQLLTC
                     YKKLLKQNVNVGLVGSQDFDKVTGELNKARVKKGKPLTEVYYEVDSTLSSGSLIPKNS
                     WLIVGGMKDELFIDAVDHEYCWRLRAAGFKVIRNKNALLAHRLGDGRFKILNILSVGL
                     PSPFRHYYATRNIFLLLNKNYVPIYWKISSLVKLIGKVFLYPIFLPNGNKRLYFFLKG
                     INDGLMGRSGKMK"
     gene            complement(19505..20692)
                     /gene="wzy"
                     /locus_tag="OO433_000564"
     CDS             complement(19505..20692)
                     /gene="wzy"
                     /locus_tag="OO433_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen polymerase"
                     /protein_id="HCR6049157.1"
                     /translation="MTSDFYNSKDKSLSVLLFFGFIFFLTRSFPFIQYSWIMEGFLCL
                     CIMSFTKKIANGIYHYPVILIFLLALFINFIYSYIKGNDIAIIIRFYIIILFILCAYF
                     CSYGTISIVKIFLYLMVLQAVIISIISIYMTKTYGIGDYSALRHYFLENDYGDVYTYG
                     SGFYRVQIKGNALIPFAFMLHIVIKDYFYYRFKNTITVILAIGTIVAGNFAYFVSICL
                     FFMYIILCSKSNSRYAKLRKIIFGVFLTVILPFFITYSIELIIMKSNGADSSLGVRWD
                     QFTVLINDLTESVSNFVIGSGLGNVIKIQTPIRDYSAYIYYELQSVYFLNQLGVILFT
                     LFLLINLLLTIKIIKYSELCVLYFLYVSYAITNPYILDSNHVAVIIVLVTLSNVLKKM
                     KAK"
     gene            complement(20748..21980)
                     /gene="wzx"
                     /locus_tag="OO433_000565"
     CDS             complement(20748..21980)
                     /gene="wzx"
                     /locus_tag="OO433_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen flippase"
                     /protein_id="HCR6049158.1"
                     /translation="MSIVKNTLWNISGYIIPSLIAIPALGILSRILGTEQFGLFTLAI
                     ALVGYASIFDAGLTRAVIREVSIYKNVHKELRAIISTSTVILTILGLIGGSVLFLSSN
                     VIVKLLNINANHVVESVKAIYIISATIPLYLLNQVWLGIFEGMEKFRKVNLIKSINNS
                     FVAGLPVIFCFFHGGLLSAIYGLVMARVLSLIVTFIFSRKLIISSGLSVKIVTVKRLI
                     GFGSWITVSNIISPIMTYMDRFILSHIVGADKVSFYTAPSEGIQRLTILPSALSRAIF
                     PRLSSELQSVKQTKILSYFIMVIGILPIVMLIIILSDFIMSAWMGPTYHGTPGIVLKI
                     LAIGFFFNCIAQIPFVSVQASGRSKITAIIHLLEVIPYLCILYIFIYHWGIVGAAIAW
                     SVRTSLDFLILLLIDTKY"
     gene            complement(21977..22534)
                     /gene="rfbC"
                     /locus_tag="OO433_000566"
     CDS             complement(21977..22534)
                     /gene="rfbC"
                     /locus_tag="OO433_000566"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6049159.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPDSDRGIVWNDPILSIKWPTI
                     EHNNYILSIKDARAKELHNMKELFL"
     gene            complement(22539..23417)
                     /gene="rfbA"
                     /locus_tag="OO433_000567"
     CDS             complement(22539..23417)
                     /gene="rfbA"
                     /locus_tag="OO433_000567"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6049160.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(23475..24374)
                     /gene="rfbD"
                     /locus_tag="OO433_000568"
     CDS             complement(23475..24374)
                     /gene="rfbD"
                     /locus_tag="OO433_000568"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6049161.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKEANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAAKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELITTTAI"
     gene            complement(24374..25459)
                     /gene="rfbB"
                     /locus_tag="OO433_000569"
     CDS             complement(24374..25459)
                     /gene="rfbB"
                     /locus_tag="OO433_000569"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6049162.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKTRFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTLEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            complement(25832..26725)
                     /gene="galF"
                     /locus_tag="OO433_000570"
     CDS             complement(25832..26725)
                     /gene="galF"
                     /locus_tag="OO433_000570"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6049163.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(26957..27952)
                     /locus_tag="OO433_000571"
     CDS             complement(26957..27952)
                     /locus_tag="OO433_000571"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HCR6049164.1"
                     /translation="MNNNVLLIGASGFVGTRLLETAIADFNIKNLDKRQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            complement(28110..29504)
                     /gene="wcaM"
                     /locus_tag="OO433_000572"
     CDS             complement(28110..29504)
                     /gene="wcaM"
                     /locus_tag="OO433_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6049165.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGHTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMSNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLVYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(29515..30735)
                     /gene="wcaL"
                     /locus_tag="OO433_000573"
     CDS             complement(29515..30735)
                     /gene="wcaL"
                     /locus_tag="OO433_000573"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6049166.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEILALQKGDTQN
                     THAAWTKYNLAARTHWLQDEPTGKIAKLRHRASQTLRGIYRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLGTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(30732..32012)
                     /gene="wcaK"
                     /locus_tag="OO433_000574"
     CDS             complement(30732..32012)
                     /gene="wcaK"
                     /locus_tag="OO433_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6049167.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMMALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(32288..33766)
                     /gene="wzxC"
                     /locus_tag="OO433_000575"
     CDS             complement(32288..33766)
                     /gene="wzxC"
                     /locus_tag="OO433_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6049168.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCLAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPQGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKPLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQFLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(33768..35108)
                     /gene="wcaJ"
                     /locus_tag="OO433_000576"
     CDS             complement(33768..35108)
                     /gene="wcaJ"
                     /locus_tag="OO433_000576"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6049169.1"
                     /translation="MVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQML
                     GGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYGLTS
                     IGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLMESFRNQPWLGFEVVGVYH
                     DPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGARVKKLVHQLADTTCSVLL
                     IPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRAEDIVLATLILLLISPVLC
                     CIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTKV
                     GNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKVKPCITG
                     WAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY"
     gene            complement(35213..36583)
                     /gene="cpsG"
                     /locus_tag="OO433_000577"
                     /gene_synonym="manB"
     CDS             complement(35213..36583)
                     /gene="cpsG"
                     /locus_tag="OO433_000577"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6049170.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTSVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSRDALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(36776..38212)
                     /gene="cpsB"
                     /locus_tag="OO433_000578"
     CDS             complement(36776..38212)
                     /gene="cpsB"
                     /locus_tag="OO433_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6049171.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSSVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(38215..39445)
                     /gene="wcaI"
                     /locus_tag="OO433_000579"
                     /pseudo
     CDS             complement(38215..39445)
                     /gene="wcaI"
                     /locus_tag="OO433_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(39442..39921)
                     /gene="gmm"
                     /locus_tag="OO433_000580"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(39442..39921)
                     /gene="gmm"
                     /locus_tag="OO433_000580"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6049172.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLPGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(39924..40889)
                     /gene="fcl"
                     /locus_tag="OO433_000581"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(39924..40889)
                     /gene="fcl"
                     /locus_tag="OO433_000581"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6049173.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQSTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(40892..>41588)
                     /locus_tag="OO433_000582"
     CDS             complement(40892..>41588)
                     /locus_tag="OO433_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6049174.1"
                     /translation="IPQKETTPFYPRSPYAVAKLYAYWITVNYCESYGMYACNGILFN
                     HESPRRGETFVTRKITRAIANIAQGLESCLYLGNMDSLRDWGHAKDYVKMQWMMLQQE
                     QPEDFVIVTGVQYSVRQFVEMAAAQLGIKLRFEGTGVEEKGIVVSVTGHDAPGVKPGD
                     VIIAVDPRYFRPAEVETLLDDPTKAHEKLGWKPEITLREMVSEMVANDLEAAKKHSLL
                     KSHGYDVAIALES"
BASE COUNT        10908 a        10556 c         9588 g        10536 t
ORIGIN      
        1 cagtgacaaa gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg
       61 gcggtaaggt cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg
      121 ttatcaccag caaaacggca accaacggcc acacagacaa attaaaagtc tcagcaatag
      181 agttatgttc agtactgtag tgctgcaagg tttgatttaa accactggca tgtggagaac
      241 gcatcaccgc actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct
      301 cacccgcgcg ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga
      361 agatataccc acctataaca gtaccaggcc acgggaacgc cccggcttcg taagtcagta
      421 ggcccgccta aattaacgca aaaaccccca cactttgtac cgaaattgca atcagcaagg
      481 cgtaaaacat gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa
      541 aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca
      601 ttgaaaacat aaattaatta accagatgaa tgttaatgag gaaattattc atgactggtg
      661 gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttcttaa tgaggatttc
      721 gataaaaaag aataaaaaaa ccggagccaa atattcattc gactccggtt gattattaga
      781 aaagatgatt atgctacgtc ttcgtactgc ggaaccgggt tacggaagct cttggtgacg
      841 caagccaggt aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct
      901 tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac
      961 tggaagtgat ctttcagcgt cttattacgc ttctcacgga tccagaactg cgagatgacc
     1021 gacaggttaa cgaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt
     1081 accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc
     1141 ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaatac gccgtcacga
     1201 cccattacgt acatcagacg cgctacgcct gcatgcgccg ccataccgga cgccagtacg
     1261 gtaatggtgg agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg
     1321 atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc
     1381 aggaagtaag ttgcaaagat gaagatcatg ccgccgatca gcgcggtcag gaaaatcgca
     1441 cgcgggatca cgcgctctgc atctttggtt tcttccgaca ggttgctgat gccgtcaaag
     1501 ccggtaaagg agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg
     1561 ccagaccaga atggacgagt gctcgccagc gtaccagcgc cttcaccttc aaatacgcga
     1621 taaacaacca tgcccagaat caccgcgatc agcaccacct gcaacacgac gattacagtg
     1681 ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcatgaa ggccaccaac
     1741 gccaccacaa acatccacga tgggatggaa ggcaccagag cttcaaaata gattttcgcc
     1801 agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata
     1861 aagccgacag tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga
     1921 tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac
     1981 gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa agtatcaaac
     2041 agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa cgtaagcgtt
     2101 ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt cattctcctt
     2161 tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac
     2221 aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa
     2281 tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt agtcattttt
     2341 tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca
     2401 taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga
     2461 tcgcactatt tgctaaaatt tcatcccgcc accagccact ggcaagtcct gcggtctgtt
     2521 cattccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc ttctcgatta
     2581 acgcgccact atcgagaaaa tgttgcgcca gataacgggg taaatagccg cagccgaggc
     2641 cgctaatttg cagctccagc ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt
     2701 cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc
     2761 gatatcgctt aataatacga cgatttaacg gctcttcttc cagggctaac ggatgatgag
     2821 gcgcgaccgc aaagacttgt tccagatcgc ccaaccgtga aaaaccaaac tcactggaag
     2881 atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc agggcatccc
     2941 atgaaccacc cagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa
     3001 aagcttcaat cagtggcgct agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca
     3061 gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttcc cgcacggtat
     3121 gcagaacttc ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt
     3181 gaccgctacg atcaagcaat tgaatattaa ggtcgctttc cagcttgtga acggtgtaac
     3241 tcagagcgga cggcgtcttg tagagtttgg ccgacgccgc cgcaaaactg ccttcttttt
     3301 ctaaggcatc gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt
     3361 gtatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc ggatcgggat
     3421 actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc
     3481 cgctgtccag actatttctg aactgcggag gttccagccc cagtaaacgg gccatctgcg
     3541 gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt
     3601 taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa
     3661 caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga tgacgccccg
     3721 gtccgaccag gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc
     3781 agtcttcgag ttcttctaat actagtccac tgttggttac cggattacgc ggggtagttt
     3841 ctttcaccat gccttgcgca tcgccataga cagatgttga gctggtaaaa ataattcggg
     3901 gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat
     3961 cgccggagcc gctacgacgt gccggaagcg taatgaccag tgcatcggca tccatcaggg
     4021 catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa
     4081 tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc
     4141 agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac ccgacaattg
     4201 cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta
     4261 tcaaaactga caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca
     4321 atgagcagtg aatcgtcggt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg
     4381 gactagaaac ctgacaaatt atgtttcagg ggttctggct tccccttacc ttcaaatggc
     4441 gttctgcggg tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc
     4501 tgccagtaca gataatcatc ccatgattcc tcagaccaga ttagtttcac tcaataatgt
     4561 ccttttctgt tcctttgcct gatttcaggc tatcgattga gtccatcaat ctccgggcgt
     4621 tagcggggga acgcagtaga taagccgtct cttccagcga gttgtattct tcgagtgaca
     4681 tcagaacaca agcctctcca ttctgacgag taataaggat cggggcatga tcttcaacgg
     4741 ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt gtacgcatgt
     4801 caatctcctc ttttgtacag ttcattgtac aatgatgggc gttaattaac tatttattaa
     4861 ttagtttgta gatcaaggta ttgtcagtga gacgaaaatc caggcttcgc tatttttggt
     4921 gccgtcagct tagaggacag tcctcttagc cccctccttt ccccgctcat tcattaaaca
     4981 aatccatcgc catagaatat ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg
     5041 ttaaaagaca tcagttgaat aaacattcac agagactttt atgacacgcg ttcaatttaa
     5101 acaccaccat catcaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
     5161 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
     5221 gcttttttat tgcgcggttg ataacggttc agacaggttt aaagaggaat aagaaaatga
     5281 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
     5341 gcgaattgct ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
     5401 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgacgacatt cccggtctgg
     5461 taatggacgg cgtggttgac ctcgggatta tcggcgaaaa cgtgctggaa gaagagctgc
     5521 ttaaccgccg cgcccagggt gaagatccac gctactttac cctgcgtcgt ctggatttcg
     5581 gcggctgccg actttcactg gcaacgccgg ttgatgaagc ctgggatggc ccgctctcct
     5641 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     5701 aaggtatctc ttttaaatcc tgcttactga acggttctgt tgaagtcgcc ccgcgtgccg
     5761 gactggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     5821 tgcgcgaagt cgaagttatc tatcgctcga aagcctgcct gatacaacgc gatggcgaaa
     5881 tggaagaatc caaacagcaa ctgatcgaca aactgctgac ccgtattcag ggtgttatcc
     5941 aggcgcgtga atcaaaatac atcatgatgc acgcaccgac cgaacgtctg gatgaagtca
     6001 tcgccctgct gccaggtgcc gaacgcccaa ctattctacc gctggcgggt gatcaacagc
     6061 gcgtagcgat gcacatggtc agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     6121 aagcgctggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     6181 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     6241 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcactgtta acgatattct
     6301 cgataacgtg aaagcacgcg gcgatgaggc cctgcgggaa tacagcgcga agtttgataa
     6361 aaccacggtt accgcactga aggtgtctgc tgaggaaatt gccgccgcta gcgaacgcct
     6421 gagcgacgag ctaaaacagg cgatggcggt ggcagtgaag aatattgaaa ccttccacac
     6481 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcca ggcgtgcgtt gccagcaggt
     6541 cacgcgcccg gtcgcttcag ttgggttgta tattcctggc ggctccgccc cgctcttctc
     6601 aacggtatta atgctggcga ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     6661 ctcaccgccg ccgattgccg atgagatcct ttatgcggcg cagctgtgcg gtgtgcagga
     6721 cgtgtttaac gtcggcggcg cacaggccat tgccgcactg gcgtttggta cggaatctgt
     6781 gccgaaagtg gacaaaatct tcgggccggg caacgccttt gtcaccgaag cgaaacgtca
     6841 ggtgagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgtt
     6901 ggtcattgcc gacagtggcg caacgccgga tttcgtggct tctgatttgc tttctcaggc
     6961 tgaacacggc ccggactcac aggtgatttt actgacgccc gctgctgata tggcgcgtcg
     7021 cgttgccgag gctgtcgaac gccagctggc agaactgccg cgtgctgaaa ccgcccgtca
     7081 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tagagatctc
     7141 caaccagtac ggcccggagc acctgatcat ccagacccgc aacgcccgcg atctggtcga
     7201 tggcattacc agtgccggtt cggtatttct tggtgactgg tcactggaat cggcaggtga
     7261 ttacgcctcc ggcaccaacc acgttctgcc gacttacggt tacaccgcca cctgttccag
     7321 cctcgggctg gcagatttcc agaagcgcat gaccgtgcag gaactgtcga aagaaggttt
     7381 ctccgcgctg gcttcgacca ttgaaacatt ggccgccgcc gagcgcctga ccgcccataa
     7441 aaatgccgtt actttacgtg ttaacgccct taaggagcaa gcatgagcat cgtgactatt
     7501 accgatttag cgcgtgaaaa cgtgcgcaac ctgacgccgt atcagtcggc gcgtcgtctg
     7561 ggcggtaacg gcgatgtctg gctgaacgcc aacgaatatc ccacagccgt ggaatttcag
     7621 cttactcagc aaacgctcaa ccgctacccg gaatgtcagc cgaaagcggt gattgaaaat
     7681 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     7741 ggtattgaac tactgattcg cgctttttgc gaaccgggta aagacgccat cctctactgc
     7801 ccgccaacgt acggcatgta cggcgtcagc gccgaaacca ttggcgtcga gtgccgcaca
     7861 gtgccgacgc tggacaactg gcaactggat ttacagggca tttccgacaa gctggaaggc
     7921 gtaaaagtgg tctatgtttg cagccccaac aacccgaccg ggcaactgat caatccgcag
     7981 gattttcgca ctctgctgga gttaacccgc ggtaaagcga ttgtggttgc cgatgaagcc
     8041 tatatcgagt tttgcccgca ggcatcgctg gctggctggc tggcggaata tccgcacctg
     8101 gctattttgc gcacactgtc gaaagctttt gctctggcgg gccttcgttg cggatttacg
     8161 ctggcaaacg aagaagtcat caacctgctg atgaaagtga ttgcccccta cccgctctcg
     8221 acgccggttg ccgacattgc ggcccaggcg ttaagcccgc aggggatcgt cgccatgcgt
     8281 gagcgggtgg cgcaaattat tgcagaacgc gaatgcctga ttgccgcact gaaagagatc
     8341 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     8401 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     8461 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     8521 gtcattgacg ccttacgtgc ggagcaagtt taatgagtca gaagtatctt tttatcgatc
     8581 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggatcgt tttgacaaac
     8641 tcgcctttga accgggcgtg atcccggaac tgctgaagct gcaaaaagcg ggctacaaac
     8701 tggtgatgat cactaatcag gatggtcttg gaacacaaag tttcccgcag gcggattttg
     8761 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     8821 tgctgatttg cccgcacctg cccgccgatg aatgcgactg ccgtaagccg aaagtaaaac
     8881 tggtggagcg ttatctcgct gagcaagcga tagatcgcgc caacagttat gtgattggcg
     8941 atcgcgcgac cgatattcaa ctagcagaaa acatgggcat tactggttta cgctacgacc
     9001 gcgaaaccct gaactggccg atgattggcg agcaacttac caaacgagac cgttacgccc
     9061 atgtagtgcg caacaccaaa gagacgcaga ttgacgttca ggtgtggctg gatcgcgaag
     9121 gtggcagcaa gattaacacc ggcgttggct tctttgatca tatgctggat cagatcgcca
     9181 cccacggcgg tttccgcatg gaaatcaacg tcaaaggcga cctctatatc gacgatcacc
     9241 acaccgtcga agataccggc ctggcgctgg gtgaagcgtt aaaaattgcc ctcggcgata
     9301 aacgcggtat ttgtcgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     9361 cactggatat ctctggtcgc ccgcacctgg aatataaagc tgaatttacc taccagcgcg
     9421 tgggcgatct cagcaccgag atgatcgagc acttcttccg ttcgctctct tacaccatgg
     9481 gcgtgaccct gcacctgaaa accaaaggta aaaacgatca ccaccgtgta gagagcctgt
     9541 tcaaagcttt tggtcgcacc ctgcgccagg ccatccgcgt ggaaggcgat accctgccct
     9601 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     9661 ctcggtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     9721 cgtcgtgttg ctggccgata aactattttt acccggcgtt ggcaccgcac aagcagcgat
     9781 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     9841 aggcatctgc ttagggatgc aactgctggg gcggcgcagc gaagagagca acggcgtcga
     9901 tttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc taccgctgcc
     9961 ccatatgggc tggaaccgcg tttacccgca ggcaggcaac cgcctgtttc aggggattga
    10021 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
    10081 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
    10141 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
    10201 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
    10261 cagggcgatt acggcaaaca gcgcgattac ggtaacgacc cgctgccgcg cttacaggat
    10321 tacgccacgc agggtgccga agtgttgcat ctggtggatc tgaccggggc aaaagatcca
    10381 gcaaaacgtc aaatcccgtt gattaaaacc ctggtcgcgg gcgttaacgt tccggtgcag
    10441 gttggcggcg gcgtgcgtag cgaagaagac gtggcggcgt tactggaagc gggcgttgcg
    10501 cgcgtggtgg tcggctccac cgcggtgaaa tcaccagaaa tggtgaaagg ctggtttgaa
    10561 cgtttcggtg ccgatgcctt agtgctggcg ctggatgtcc gtattgacga gcaaggcaac
    10621 aagcaggtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
    10681 gaaacctatc tgcccgtcgg cctgaaacat gtgctgtgta ccgatatctc gcgcgacggc
    10741 acgctggcag gttctaacgt ctctttatat gaagaagtgt gcgccagata tccgcaggtg
    10801 gcatttcagt catccggcgg cattggcgac attaacgatg ttgctgccct gcgtggcact
    10861 ggcgtgcgcg gcgtaatagt tggtcgggca ttattggaag gtaaattcac cgtgaaggag
    10921 gccatcgcat gctggcaaaa cgcataattc cgtgtctcga cgttcgtgat ggtcaggtgg
    10981 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgccg ctggcaaaac
    11041 gctacgccga agaaggtgca gacgaactgg tgttctacga tatcaccgct tccagcgatg
    11101 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgttct
    11161 gcgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
    11221 ataaaatttc catcaactcg cctgcgctgg cggatccgac gttaattact cgcctggcgg
    11281 atcgttttgg cgtgcagtgt attgtggtgg gtattgatac ctggtacgac gctgaaaccg
    11341 gtaaatatca tgtgaatcaa tataccggcg atgaaagccg cacccgcgtc actcagtggg
    11401 aaacgctcga ctgggtacag gaagtgcaaa aacgcggtgc cggagaagtc gtcctcaaca
    11461 tgatgaatca ggacggcgtg cgtaacggtt acgacctcga acaactgaaa aaagtgcgtg
    11521 aagtttgcca cgtcccgcta attgcctccg gtggcgcggg caccatggaa cacttcctcg
    11581 aagccttccg tgatgccgac gttgacggcg cgctggcagc ttccgtattc cacaaacaaa
    11641 taatcaatat tggtgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
    11701 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggac ttatgccggt
    11761 gattgtgcaa cacgcggtat ccggcgaagt gttaatgctg ggctatatga acccggaagc
    11821 cttagacaaa accatagaaa gcggcaaagt cgccttcttc tcgcgcacta aacagcgact
    11881 gtggaccaaa ggcgaaacgt cgggcaattt cctcaatgtg gtgagtattg caccggactg
    11941 cgacaacgac acgttactgt tactggtgaa tcctatcgga ccaacctgcc ataaaggcac
    12001 cagcagctgc ttcggcgaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
    12061 gctcgccgaa cgcaaatctg ccgacccgga aacctcctac accgccaaac tgtatgccag
    12121 cggcaccaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
    12181 gacagtacat gaccgctttg agctcaccaa cgaggcgtct gatttgatgt atcacctgct
    12241 ggtgctgttg caggatcagg atctggactt aacgacggtg attgagaacc tgcgtaaacg
    12301 gcatcagtga gttgcggggt aagcggatgc gatattgttg tagcatccgg aaaaaaaaac
    12361 gggcaaggtg tcaccaccct gccccttttc tttaaaaccg aaaagattac ttcgcgttgt
    12421 aattacgcag agcgttacgc cccaagacaa tccccgcgcc aaccatgccg cccagcagca
    12481 ccgccagaat caaggtaatt gcctttttcg ggctatcgcg acgaataggt aacgtcggtt
    12541 tcattacata gcggtaagca tgaatatcaa gtttatcaac gtcaagattg tcgatatcca
    12601 gcaggttttg acgagtctga tagtagtttg gtgagaacac caacggacga gtcgcttcat
    12661 gcttaatcat cgactccagc gcttcgctcc ccaaaaggaa caacgtatct tgtgtcacat
    12721 cttcagtctg ctgaacctgt ggctttgtca cctgcgcctg attcgcatac tgcaacgctt
    12781 cctgaatctg acggatacgc agatctttct gctcctgcgc aactacttcc tgagttttca
    12841 gagaatcctg caagtttttc atacgcagcg cgatgttgtc tttcaggtct tttcccagtt
    12901 cttcatttac ctggtcgtca acttgctgga tatacttcgc cagctgcttc tgagcacctt
    12961 caggtgtttc accaacataa gacaccgcta aaggtaatga ctgatttttg acggtaggct
    13021 caatggtcag cttttctgct tcttcctggt tatccagcgt ttccgctaat gctgagaatg
    13081 cagtgctgta acgtccgata agtgaagcct gaatgtcgga gactttcggt acagccgatc
    13141 cgtagatcac attcatcgcg ttggtataac cagcgatttg ccccacatca ggttgagtaa
    13201 cgatagctgt ggaagtccac ttttctttgg caacggccag ataccctata gccagggcaa
    13261 tagcaacaat gacagagatg ataattgtca tcttgccacg ccacaactgc accagtaaat
    13321 caatcaaatc aatctgttcc ggatcatggt tttgcccaga aacattatta ttttctactc
    13381 tcatcattac cctaacggac gaaataattt taagtcgcca tagtttagcg ataatcgcca
    13441 tgaatgctat aggataaatg ataaaaataa tgaattatta caaagggaac ataagagaat
    13501 cagggcgaaa atcgccatga taacaggatg ttagtcactg ccaaagagat cgcgggtgta
    13561 gactttgtct gccacatcct taagctcttc tgccatacgg ttggagataa tgacgtcggc
    13621 ttgtatagtc agcttagaac agctttgatt gtaaaatgag cacgttacca gaaaaaacag
    13681 ccaagtttag aactgatacc gattatcttt ttttcttgtc taacggtata atttaacttt
    13741 cagttatgcc agatgaagat tggctatatt ctagcctgag cgaggattat aaatgaaatt
    13801 tctggttacg ggagctgctg gctttatcgg tttctatcta agtaaacggc ttcttgcagc
    13861 tggtcatcag gttgtaggca ttgacaactt aaatgattat tacgatgtca acctcaaaca
    13921 agcacgactt gatttactca agcacgacaa cttcagtttt tataaaattg acctggccga
    13981 tcgtgagaaa atggcggcac tgtttgcaga cgagcggttc gaacgcgtaa taaacctcgc
    14041 tgcgcaagcc ggtgtacgtt actctcttga aaatcccaat gcatatgcag atgcaaacct
    14101 gattggattc ctgaacatac tagaaggatg tcgccataat aatgttcagc atctacttta
    14161 cgcttcctcc agttctgttt atggcatgaa ccgcaagatg cctttctcta cagatgactc
    14221 tgtagatcat cctgtttcgc tttatgcagc aactaaaaaa gcgaatgaac tcatggccca
    14281 tacatattct catttgtatg gcttaccgac cacagggctg cgtttcttta cggtttatgg
    14341 tccatgggga cgtccggata tggcattatt taaattcact aaagccatgc tggaaggtaa
    14401 aagcattgat gtttacaact tcggcaaaat gaagcgtgac tttacttaca ttgatgatat
    14461 tgccgaagct attattcgct tacaggatgt tattccagaa aaaaacccac agtgggctgt
    14521 agaaacaggc tcgcctgcaa caagttcagc accatatcgt gtctataaca ttggtaatag
    14581 ttcgcctgtg gagttgatgg actatatcaa tgcgttagaa gaggctctgg gtattgaagc
    14641 caacaaaaat atgatgcctc tccaacccgg tgatgtactg gaaaccagtg ctgatacaaa
    14701 agcactgtat gacgtaatag gattcaaacc tgaaacgtca gttaaagaag gggtaaagaa
    14761 ctttgtagaa tggtatcgta acttctataa agtttgattt tacaaaacca taagaaaagg
    14821 ccctaattta ttagggcctt ttcttagaat gaaacaaaat aattaatcat tgccaaacaa
    14881 gtcgcgcgta taaactttat ctgctacatc agccagatcg gcagacatac ggttagaaat
    14941 aataacatca gcttcttgtt tgaacgcatc cagatcacgt accacgcgcg accggaaaaa
    15001 atcgtcctct ttcatagctg gctcataaac gattacaggc acacctttcg ccttgattcg
    15061 cttcataata ccctgaatcg aggaagcacg aaaattgtct gaaccattct tcataatcaa
    15121 acgatagacg ccaacaactt tcggtttacg tgcaaggata gaatcggcaa taaaatcttt
    15181 gcgcgtgcgg ttggcgtcaa caattgccga gatcaggtta ttcggcacag actggtaatt
    15241 tgccagtaac tgcttagtat ctttcggcag acaataacca ccataaccga atgacgggtt
    15301 gttgtagtga ttaccaatac gcgggtcaag gcatacgccc tcaataatct ggcgtgaatt
    15361 aagtcccagg ctttcagcat aactatcaag ttcattgaaa tacgctacac gcatcgccag
    15421 ataagtgttc gcaaaaagtt taatcgcctc agcctcggtt gagtcagtaa acaatgttgg
    15481 tatgtcttgc ttaatggcgc cttcctgtaa taacgcagca aaacgtttag cgcgttcaga
    15541 ctgctcgcca atcacaatgc gtgatgggtg taagttatca taaagtgctt taccttcacg
    15601 caaaaactca ggcgaaaaga tcacattttc aataccaaaa cgttctttaa tggactctgt
    15661 aaaaccaaca gggatagttg attttataat cattaccgcg ttgggattaa tttctgtcac
    15721 atcacgaatg accgcttcca cgcttgaggt attaaaataa tttgttttcg gatcataatc
    15781 ggtaggtgtg gcaataataa cgtaatcggc atttttatac gcgtcatact tatctgtcgt
    15841 agcgcggaaa ttgagatctt tagtcgccag atactcttca atctccttat caacaagcgg
    15901 tgactgcctc ttgttaagca tgtccacttt ggcctgaacg atatccagtg caaccacttc
    15961 gtggttttgc gcaatcagaa taccatttga aagaccaaca taacctgttc ctgaaattgt
    16021 tattttcatt agctctgact tcttccggtt aaacatttag agtggtcatt aatccaccac
    16081 cacagcttca tctactgcgg gatttttaac gctgatgtag atgaactgtc aaggcagcga
    16141 tcctgctgtg cggcgctgta ttatatcgcg tttttaacta taaattataa aaaaaggccc
    16201 taacctgccg ctttgtataa taaaaaagcc cggagggttt ctccgggcct tgttttgatt
    16261 aattgattta aatcagatta atccagccat tcggtatgga acacaccttc tttatcaatg
    16321 cgcttataag tatgcgcacc gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga
    16381 acagcggcgc ggtagctgtc gtaataggca accgcagcgg cgaaggtcgg caccgggata
    16441 ccgttctgta ctgcgtaagc gacgacatcg cgcagcgcct gctggtagtc atcggcaatt
    16501 tgcttgaagt aaggagccag caacaggtta gcgatctgcg gattttcggc ataagcatcg
    16561 gtgattttct gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt
    16621 tcaccgtagt tcagatccca gttgtactct tcagacgcag cgcgtagctg agagaagccc
    16681 tgagcgtaag aaacgatttt gcccagatac agcgcacggc gaactttttc gatgaactca
    16741 gcattgtcgc cagctggctg cgcttgcggg ccagagagaa ctttagatgc ggcaacacgc
    16801 tgctctttca gagaagagat ataacgtgca aacacagact cggtaatcag cgacagcggt
    16861 tcgccgagat ccagcgcgct ctggctggtc catttgcccg tacctttgtt tgctgcttca
    16921 tccagaatca catcaaccag gtagttaccc tcttcatctt ttttggtgaa gatatctttg
    16981 gtgatgtcga tcaggtagct gctcagttca ccgttattcc actcggtaaa ggtctgcgcc
    17041 agttcttcgt tggtgaggtt caagccacct ttaagcagag aataggcttc agcaatcagc
    17101 tgcatatcac cgtattcaat accgttgtga accatcttca cataatgacc tgcaccatcg
    17161 gcaccaatat aggtaacgca cggttcgccg tcttcagcca cagcggcgat tttggtcagg
    17221 atcggcgcaa tcagttcata agcttctttc tgcccaccag gcataatgga aggacctttc
    17281 agcgcacctt cttcaccacc ggaaacaccg gtaccgataa agttaaagcc ttctgcagaa
    17341 agctcacggt tacgacgaat ggtgtcatgg aagaaggtgt taccaccatc aatgatgatg
    17401 tcacctttat cgaggtatgg cttgagggaa tcaatagcag catccgtgcc agcacctgct
    17461 ttcaccatta acaggatgcg acgaggcgtt tccagagatt caacaaattc tttcaccgta
    17521 tagtaaggaa ccagtttctt gcctggattt tcggtaatca cttcttcggt cttttcacgg
    17581 gaacggttga aaatagagac ggtataacca cggctttcga tattgagcgc aaggttgcgc
    17641 cccatcactg ccataccgac gacgccgatc tgttgctttg acattgttta ctcctgtcag
    17701 gataccgctg ggtggtatgc gggttatgct taattataga atatgcctaa taaaaataaa
    17761 tccataacac ttaatcagaa aattattatt atcgattcct aacgattgaa tacatcagct
    17821 cctttaattt agatggcatt atacgaaaaa atgttctcaa catagcatta cttattaatt
    17881 cattttttcg aataaaacca attttatatt gataatacaa tactttatac tcgtacaata
    17941 aatatgacaa tccacgtcga gccataagat tacgaccagt tcgcattttt aataaaatat
    18001 ctggaagatt tgcaaatctt gcattatgta caattaatag gctccacaat gcaaaatctt
    18061 gagattttct gaatggagga taaccaccaa cagctaatac tgtattcttt ctaaaaatta
    18121 cagaaggatg gctaactgcg cttcgtttcc tcgcgaattt aactatttct ctatgttcga
    18181 gaggcacttt gcgtgttgaa ataaactcct cagtaacagt ttcaatttca tcaataaaac
    18241 tgccacatac atctatttct gaattattaa tcataaaaga aatttgtttc tcaaaccgat
    18301 gaggcaaaga aatatcatca gcatccattc ttgccactaa ctcattccta caagccttta
    18361 atccttcatt taaggcatta gccaatccaa catttctagg taaaggtaca aatgttacta
    18421 ttttattgcc aacatcatca atgaatgaat ttataatatc gatgtgtgtt tgatggagtt
    18481 ctccatctgc aacaattact atttgatctg gcttaagtgt ttgatcgtga aaaatagagc
    18541 gtagagccac ctcaaaaaat tgcggtagat catttttata aatgctaatt aaaactgaga
    18601 atttttctaa tctatgattc atttcatttt accacttcga cccattaaac cgtcattaat
    18661 gccttttaaa aaaaaatata accttttatt accatttgga aggaaaatag gatataaaaa
    18721 aacctttcca attaatttaa ccagactaga aattttccag tagatgggta cataattttt
    18781 atttaataaa agaaagatat ttcgagtagc ataataatga cgaaatgggc ttggcaaacc
    18841 gacagaaaga atatttaaga tcttaaatcg cccatctcca agtctatgtg caagtaacgc
    18901 atttttattc ctaattactt taaacccagc agctcttaat ctccaacaat attcatggtc
    18961 taccgcatcg ataaaaagct catctttcat tcctccaaca atcaaccaac tattttttgg
    19021 tattagactg ccagaactta atgtactatc tacctcataa taaacttctg taagtggttt
    19081 cccttttttt acccttgctt tatttaattc accagttact ttatcaaaat cttgtgaacc
    19141 aactaaacca acattgacat tttgtttaag caattttttg taacaagtaa gtaactgctc
    19201 taccatctta ggatcaggaa tactatcctg atccatttgc aatataaaat cagcgccatt
    19261 ttcaaaagcc catttcattc ctatactttg ggcttctgct atgcctaaat tatcattgaa
    19321 attgaatatt tttacatcgc ctgaagaatt ttcagcatat ttataaccat ttgtagagtt
    19381 attgcaaacg acaactttag taacttgtct caacaataat tcaaccgcat tttttaaatc
    19441 attatgttct gggttgtaag caaccaaaac ggcatataca gtgtccatct tcaccttaaa
    19501 accttcattt agctttcatc ttttttagaa cattacttaa tgtcactaat acaattatta
    19561 cagcaacatg gttagagtct aaaatataag gattagtaat tgcataagaa acatatagaa
    19621 aatatagcac acacaactca ctgtatttta tgattttaat cgtgagaagg agattaatta
    19681 ataaaaacaa agtaaataaa ataacgccaa gttgatttaa aaaataaact gactgcaatt
    19741 cataatatat atatgcacta taatcacgga taggagtttg aattttgatg acattaccca
    19801 aaccagaacc tataacaaaa tttgatacag actctgtaag atcattaatt aatacagtaa
    19861 actgatccca tctaactcct aaagaagaat cagctccatt tgatttcatg attatcaact
    19921 caattgaata tgtaataaaa aaagggagaa tcacagtaag aaaaacccca aaaataattt
    19981 tccttaattt agcgtatcgt gagttagatt tagaacatag tataatatac ataaaaaaca
    20041 agcatatcga aacaaaatat gcaaaattac cagccactat agtacctata gccagaataa
    20101 cggttattgt atttttgaat cgataataga aataatcttt tatgactata tgcaacataa
    20161 aggcaaatgg aatgagagca tttcctttaa tttgaactct atagaaacca cttccatatg
    20221 tataaacatc accataatca ttctccaaaa aataatgtct tagtgctgaa taatcaccaa
    20281 taccatatgt ttttgtcata taaatactaa tgatggatat aataaccgcc tgtaatacca
    20341 ttaaatataa aaatatttta acaatcgaga tggttccata agagcagaaa taagcacata
    20401 atataaataa tatgataata taaaacctaa ttattatcgc tatatcgtta cccttgatat
    20461 aggaataaat aaaatttata aaaagagcta atagaaatat taaaataaca ggatagtgat
    20521 atattccgtt tgcaattttc tttgtaaatg acatgataca aagacataaa aacccctcca
    20581 taatccaact atattgaata aatggaaagc tacgtgtaag gaaaaatata aacccaaaaa
    20641 acaaaagaac acttaaactt ttgtcttttg agttataaaa atcagaagtc atgtttgcac
    20701 tctaattaga tgggcttgag gaagtaatcc ctaaaatcaa ttcgctatta atatttcgta
    20761 tcaattaata ataatatcaa aaaatctaac gatgttctta cagaccatgc tattgcggct
    20821 ccaacaattc cccaatgata aataaaaata tataatatgc ataaatatgg gataacttcg
    20881 agcaaatgaa taatagctgt aatttttgat cttccactag cctgaactga aacaaatggg
    20941 atttgtgcaa tgcaattaaa aaagaaacct attgcaagaa tttttaatac tatacctggc
    21001 gtcccatgat atgtaggtcc catccaagcg gacattataa aatctgataa aataattatc
    21061 aacattacaa ttggaagtat accaataacc attataaaat atgataatat tttagtttgc
    21121 tttaccgatt gcaattctga acttaatctt ggaaaaatag ctctggacaa cgcacttggt
    21181 aatatcgtta agcgttgtat accttcagac ggagcagtat aaaaagaaac tttatcagcc
    21241 cccacaatgt gtgaaagaat aaaacgatcc atatatgtca taatagggct aataatattg
    21301 ctaactgtta tccagcttcc aaagccgatt aatcttttaa ctgttacaat ttttacagac
    21361 agcccagatg atattattag ttttcgacta aatataaagg tcactataag tgataagact
    21421 cttgccataa ctaaaccata tatagcactt agtaatcctc catgaaaaaa acagaaaatc
    21481 actggtaatc cagccacaaa agagttgtta attgatttta ttaaatttac ttttctgaac
    21541 ttttccatcc cctcaaaaat ccccaaccag acttggttta acaagtataa gggtatggta
    21601 gctgaaataa tatatattgc tttgacagat tctacaacat gattcgcgtt aatgtttaat
    21661 aatttaacaa ttacattgct actcaaaaat agtacactac cgccaatcaa gcccaatata
    21721 gttagaatta ccgttgaagt tgaaatgatc gctcttaatt ctttatgaac atttttatat
    21781 attgatactt ctcttataac agctctggtc aatccagcat caaaaatact tgcatatcca
    21841 actaaggcaa tagctaacgt aaaaaggcca aattgctcgg tccctagaat tctagacagt
    21901 atacctaacg caggaattgc tattaatgat ggtataatat acccacttat attccataaa
    21961 gtattcttta caatactcac aaaaataatt ccttcatgtt atgcaattct ttagcccttg
    22021 catctttaat cgataaaata taattattat gttctatcgt cggccatttt atgctcagaa
    22081 taggatcatt ccatacaatc cctctatcac tatcaggatg ataatagttc gtcgttttat
    22141 ataaaaattc cgcagtctcg ctcagcacca aaaaaccatg tgcaaatccc tcagggatcc
    22201 acaattgccg cttattctca gcagataaat tcaccccaac ccatttacca aaggtaggcg
    22261 acgatttacg aatatcaaca gctacatcaa aaacctcacc aacaacgcaa cgtaccagtt
    22321 tcccttgcgc ataaggttct aactgataat gcagcccgcg taaaacacct ttactagact
    22381 tcgaatggtt atcctgaaca aattcaacct tacgtcctac agcttcttcg aaaactttct
    22441 gattaaagct ttccataaag aaaccacgct catcaccaaa aactttcggc tcgaaaatta
    22501 acacatcagg aatttctgtt ttaattacgt tcattttatt aataaccttt aatcattttc
    22561 agcagatact gtccataagc attttttttc agcggctccg ctaatgcttt cacctgttca
    22621 gcatcaataa accctttacg gtaagcaatt tcttctgggc aggaaacctt tagtccctgg
    22681 cgctcttcaa tggtggcaat gaagttgctt gcttcaataa gactctgatg tgtccccgta
    22741 tccagccatg cataaccacg ccccatcatg gcaacggata aacgcccctg ttccatataa
    22801 atacggttaa tatcggtaat ttccagttca ccacgggcag aaggcttaag gtttttcgcc
    22861 atttcgacaa cgtcgttatc atagaaataa agcccggtta ccgcataatt actttttggt
    22921 tgtagcggtt tttcttccag gcttattgcc gtaccgtttt tatcaaactc aacgacgccg
    22981 tagcgttcag gatcattaac gtgataggca aataccgttg caccactttc tttgttaaca
    23041 gcgacatcca ttaacttcgg cagatcatga ccgtagaaga tattatcacc aagaaccaaa
    23101 gcacaatcat caccaccgat aaactcttca ccgataataa acgcctgcgc aagcccatct
    23161 ggagtcggtt gcactttgta ctgaagattt agcccccact ggctaccgtc acctagcagt
    23221 tgttgaaaac gaggagtatc ctgtggcgta ctaataatca gaatatcgcg aatacccgcc
    23281 aacatcagtg tagagagcgg gtaatagatc atcggcttat cataaatagg taatagctgt
    23341 ttactgacag ccatagtcac aggataaaga cgtgtaccag aaccacccgc taaaataata
    23401 cctttacgcg ttttcatttc atcattcctt ttaattcatc ttgctccacc atcacgaaca
    23461 agatgcaaaa actattaaat tgctgtagtc gtaattaatt cgttgagcat tcgtttcaca
    23521 ccaacctgcc agtcaggcaa gacaagcgca aagttctgct gaaatttttc tgtattaagg
    23581 cgagagttat gtggacgacg agctggtgta ggataggctg ttgttggtac tgcgttgagc
    23641 ttgttgagtg caaggggaat acctgctttg cgcgcctctt caaaaaccag cgcagcataa
    23701 tcgtgccagg ttgtggtacc actggctacc agatggtaca aacctgcgac ttccggttta
    23761 ttcagtgcca cacgaatagc atgtgccgta caatcagcca gcagctcagc acctgttggc
    23821 gcaccaaatt gatcatttat cacagccagt tcttcgcgct cttttgccag acgcaacatc
    23881 gttttggcga agttatttcc tttagctgcg tatacccagc tggtacggaa aataagatgc
    23941 ttcgcgcaat gttcctgtaa cgctttttct ccggctaact tggtttcacc gtaaacattt
    24001 agcggtgcgg ttgcatccgt ctccagccat ggcgtgtcgc catttccagg gaatacgtag
    24061 tcagttgagt aatgaattac ccaagcccca acttcattag cctcttttgc aattgattca
    24121 acactagtcg cattgagtaa ttgtgcaaat tcgggttctg actcagcctt atctactgcg
    24181 gtgtgagccg cagcattaac aataacatca ggtcgaattc ttttgactgt ttcagctaca
    24241 ccttcaggat tactaaaatc accacaataa tcagtggagt gaacatcaag agcaatcaaa
    24301 ttacccaaag gtgccagagc acgctgtagt tcccaaccta cctgccctgt tttgccgaaa
    24361 aggaggatat tcattactgg cggccctcat agttctgttc aatccacgat tgataagcac
    24421 cacttttcac attatcaacc cattttgtat tggacaggta ccattccaat gtcttccgaa
    24481 tcccgctctc aaacgtttcc tgcggtttcc agcccaattc gcggctaatc ttctctgcat
    24541 caatcgcata acggcgatcg tgtcccgggc gatcggcaac ataagtaatt tgctcgcggt
    24601 aagatttctc tttcggtaca atctcatcca gcaaatcaca aatagtgagc actacatcga
    24661 tgtttttctt ttcgttgtgt ccaccaatgt tataagtttc acccgcttta ccttcggtta
    24721 cgacggtata taacgcacgc gcatgatctt caacatacag ccagtcacga atttgatccc
    24781 ctttgccata aataggtaat gccttacctt ccagagcatt cagaataacc aatggaatca
    24841 atttttccgg gaaatgataa ggaccataat tattagagca attagtcaca atggttggta
    24901 aaccataggt acgtttccac gcgcggacta aatgatcgct ggatgctttt gaagcggaat
    24961 aagggctgct tggcgcgtaa gctgttgtct ctgtaaataa gggtaattct tctgtattat
    25021 ttacctcgtc aggatgaggc aaatcaccat agacttcgtc agtagaaata tgatgaaaac
    25081 ggaatctagt tttcttgtcg ctatcaagag cagaccaata attgcgagcg gcttccaaaa
    25141 ggacatatgt accaacaata ttggtttcaa taaatgccgc aggacctgta attgaacggt
    25201 caacatggct ttcagcagcc aggtgcatca ctgcatctgg ctgatgctga gcaaaaatcc
    25261 gtgccattgc agctgcatcg caaatatccg catgttcaaa aacatagcgt tcagaatcag
    25321 aaacatcagc aagtgattcc aggtttccgg cgtacgttaa tttatcgaca ttaacaacac
    25381 tatcctgcgt attatttata atgtgacgaa ctacagcaga accaataaat cctgcgccac
    25441 cagtaacaag tattttcacc taatttattc catattgctt cagagcatgc tgtgaaataa
    25501 gcggctctca gtttgattaa tagaagtatt aatgcacgct accgcccctg gctttacagc
    25561 taccagagca ctgcatgcat gcctacgatg tgacgagcgt tacccactcg cgctaaaccc
    25621 gaaaaattca aaagctaatt gtcttaccaa tccgctctgg aaacaaggaa aatcctggaa
    25681 aactttgact aaaatcctat tgctaactcg ttgttattct gattgtttat ataaaacaac
    25741 ggcaggaata ttcgcaacaa attactttca ccacgaatct tcactgccgt tataattttc
    25801 ttatcaaccg ttacatccgg tcagattttc attattcgct taacagcttc tcaatacctt
    25861 tacggaactt cgccccttct ttcaggttgc gcagcccata cttcacaaac gcctgcatat
    25921 agcccatttt tttaccgcag tcgtagctgt cgccggtcat cagcattgca tcaacggact
    25981 gttttttcgc cagctcggca atggcatcag tcagctgaat acgtccccat gcaccaggct
    26041 gagtacgttc aagttccggc caaatatcgg cagaaagcac atagcgacca acggccatga
    26101 tgtctgagtc cagcgtctgc ggctgatccg gtttttcgat aaattcaaca atgcggctga
    26161 ctttaccttc gcgatccagc ggttctttgg tctggatgac ggagtattca gagaggtcac
    26221 ccggcatacg ttttgccagc acctggctac ggcccgtttc attgaagcgc gcaatcatgg
    26281 cagcaaggtt gtagcgtagc gggtcggcgc tggcgtcgtc gatcacaacg tctggcagca
    26341 ccacgacaaa tggattgtca ccaatggcgg gtcgtgcaca caaaatggag tgacctaaac
    26401 ctaaaggttc gccctgacgc acgttcataa tagtcacgcc cggcgggcag atagattgca
    26461 cttccgccag tagttgacgc ttcacgcgct gctcaaggag agattctaat tcataagagg
    26521 tgtcgaagtg gttttcgacc gcgttcttgg acgcatgagt taccaggagg atttctttga
    26581 tccctgcagc cacaatctcg tcaacaatgt actgaatcat tggcttgtcg acgatcggta
    26641 gcatctcttt gggtatcgcc ttagtggcag gcaacatatg catcccaaga cccgctaccg
    26701 gtataactgc ttttaaattc gtcattattt tcctacctct aaggggctga tagtgcgtaa
    26761 attattgtca taggttagcc aaacggtatg gctatatacc aagcataact ttgattaaac
    26821 cttacgataa cactacacac catcagcatc tgggttactc ggattactcg gaaatccaca
    26881 tactgataat ttaatcagta cctctttccg aataatcgta gtccaacctg gtcctttttt
    26941 ctctgactcg tctgcattac tcagaaacaa acgttatgtc gtcttttttg gcatggacga
    27001 attcatactg cagagttcga tccagacctt gcgacagcgt atacggtgca acaaaacctg
    27061 aagaatgcac tttcgttgcg tcaaactgtg ttgttgcgca gaattttttc acgcgcacag
    27121 agctgacagc gtattttttg cccgtaattt tgctcaggat atcaaagcaa tatccaccca
    27181 gcattcctag tgggtaaggc aagtgcatag aagggatctt tttgttcagg ctttgttcaa
    27241 cttcagcaac caactggttc atgttcaggt ctggcttatc aacatagtta taaacctcat
    27301 aacctgcggc aacattcttc agtttgtact tgataaactc aacaatgttt ccaacataag
    27361 ccatggactt atagttagtc cctgcgccca ccatcataaa cttgccgcca gcgatctgtt
    27421 tcagcaagtt atagacgtta ccgcggttgc gttcaccgaa gataacggta ggacggatga
    27481 tggttaatga acgttctgtt ggtgctttgt tataccattc acgcagcact tcctctgcct
    27541 gccacttact tttgccgtag tggttgaaag ggtcgtgtgg atggttttcg tcagggttgt
    27601 gtttgttcaa accataaaca gcaacggaac tggtaaagat gatattttta acgccatttt
    27661 tttccatggc cgccagcaca ttgcgggtac cctgaacgtt gacatcataa tagagagaag
    27721 tagggctgac gtcatcgcgg tgttccgctg ccagtagtac aacagtgtca aaaccggcta
    27781 acgcctggtc gagtgcctgt tgatcacgaa catcaccaat ctgtgtgatt tctggataaa
    27841 agtggctctg ccgtttgtcc aggttcttga tattaaagtc agcaattgcc gtttcaagta
    27901 gtcgggttcc tacgaatccg gaagctccta tgagcaaaac gttattgttc ataaatcact
    27961 ttagtctggt tgttacgtaa gaaacacaag ataaagatga gtaccttccc tgagtagtca
    28021 atgctgccca gccccagctt taacagttag tgtgaggatt ataatctttt agaacattat
    28081 atccagtaag tttatgaatg gtcgcaaatc tactctctcc gttccggcaa tctaaagtta
    28141 atcttttcca cattaacaat atggtgatta atcctgtcga tgtcgacgga gctttgccct
    28201 ctttcattca tcgcatgaac atttgcaagt gacagcagtg tttctttttt cgccataaag
    28261 acaccgcgta cgtctttgcg catgtcgaag ttcatgctca atgctggtcc aactgaggat
    28321 tcctgcatca ccttgatatt acgcataaaa agatgttgcg gtttgttgtg taactccagc
    28381 gaggcacgct tcatctcaat gttagtcagt gccacaaagg agacagcatt cccggcggag
    28441 atttggatgc cgcgcaattt ataaacaaga tgggtgttat ccagttgaat attattcact
    28501 cggaaatttt gcggtatcga gagatatttg cctttaatta ccccatagcc gattaacatc
    28561 ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc gtaaatagca
    28621 actgtcgcgt tatcaatgcc tgctttctta ctgaaatccg gcgtgatatt gcgggctttg
    28681 atattacgga taacaaaatg tttgccattt tcaacatgga tcaactgccg acaatccgat
    28741 cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta gttattatcg
    28801 taggtactcc ccgcaaggcc tatgccaatt ccccagttga ttttgccatt agtacagttg
    28861 atgcgctcga tgacatggtc ggagatcaaa atatcactgt cgttaattgc cacgttccat
    28921 tcaatggcgt cgccctgtaa gtcgctgaac ttacaattgg tgatgttggc accgataatc
    28981 tggttatgaa atccctggcg taagatggcg taattagcgt ggctgacagt gaggttatcg
    29041 atggtcaggt tgctcatgac ccgtttgttt ttgccgccga tataaatctg cgttaccggg
    29101 ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag aaccacgcac atccaaggtg
    29161 atgttatgca tactgccgcc ctcctcccct gtcacctggc tgccgtcctg taagacaaat
    29221 cgccctctgc cgttgccgcg caagcttcca agaatgtgta acgttttacc gggagggata
    29281 aagatgccgg tgttgatatt gtcacaaacc aatccggcag gcacgacgac cgtatgccct
    29341 tcgctgaagg cttgtttaaa tgaggcgatc cagtcgtgtg ggttgtagtc gttaatgtta
    29401 acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat ggaggaaggc aagcgccgag
    29461 cttgccgtca ggaaggtgcg tcgggagagt gttttaaatg gcatgcgttc tcctctataa
    29521 agcttgcagc aagctggcga gttctcgatt gatcacctgc tggttaaaat cgtgttcgac
    29581 tttttcgcgc gcacgtttga caaccggagc cagttcgtcg gtgcccagtt ggctaaacgc
    29641 cgccagtcgt tgcgccagtg cgcgagcatc gttctcaggc accagccagc cggatttgtc
    29701 agcctccacc agttccggta ttccgctatg cagagtagaa accaccggaa taccgaccgc
    29761 catcgcttcc attagcgcca ccggaatgcc ttccatatca ccatccgcgc ccgttaccga
    29821 tggcaacaga aagacatccg cgtcgtcgag catcgctttc acttcgtggc tcggtttaaa
    29881 gcccggcatc tctaccacat cttccagttg atattgttcg atgagggtac gcaggcgtcg
    29941 ttcccacggg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct ctttcaactg
    30001 acggcaggct tcaatcgcca catgcaggcc ttttttctcg gttaagcgtg cgacggaaat
    30061 gatttccagc ggcgttgcgg gcgctttcac cggacgcggg ctaaagcgcg tcatgtccac
    30121 gcccatacgc gatacggcga ttttttccct cgggcagccc attttttgca gccttccggc
    30181 ccacaaatcg cttatcggta acatcaggtc gccacggcga aacagttgtt gatattcggg
    30241 agtgtaatgg ttgagcactt cccgactgga aatatcaatg ccgtggaaaa tggtggcaat
    30301 tttgccgcga atgacaccca gttcccgtag ttttgctgcg gttacccccg ccgggccaaa
    30361 atgagcgatg aacacatcgg catgaagcgg tgttgcgacc tggccgcaaa tggcagacaa
    30421 aatcaggttc cgcgactcgg caccatagcg tttgaggttg agcgcctgcc aggtattttt
    30481 acgataaatg ccgcgtaacg tctggctggc gcggtggcgc agtttcgcta ttttgcccgt
    30541 aggttcgtcc tgtaaccagt gggttctggc ggcaaggttg tatttcgtcc atgccgcgtg
    30601 ggtgttttgt gtgtcgcctt tttgcagcgc gagaatctct acctcaaatc ccatatcaat
    30661 aaacgcggta atttggttaa ggacgaaggt ttctgacgac agcgggaatt tcagtaaaaa
    30721 gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac catctgcatt
    30781 cctgtctgac gctcacgact gacggcttca ctaagtcgcg cattcagtgc cggaagctgg
    30841 cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcta ataaatgacg gatatcaatt
    30901 gccatctccg gtagtcccag ctgctgcata atcccggcgg atttatgttc gtagttgatg
    30961 gcaattgccg gagtggcaaa attcatcgag ataatggcag agtgcaggcg cgtaccgacg
    31021 gtgagttcac aggcaccgag aattttgccc atttccagat cgttgagttc atccatcacc
    31081 acatggtaac gggcaggatc gctgatgtgc tggcgaagat tgagcgccat catgcggtcg
    31141 tctttgttat agctgtcgat acccgtacag gtggagagtg caatgacctg atagccttcg
    31201 tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg
    31261 aggcgtttgt caaacggtgc cagttcgcgc agggtaattg ctaccgtttt ctgttgtgcg
    31321 gcaacgtcca gccagtgttg aacagcatag ctggcggtga agtcttcggt gtggtgatcg
    31381 accagccacg cggtatcgac gccatgttcc acttttgcgg tagtgatatt gctgcgtttc
    31441 atcagatcaa gactgaccga ttcgcgcagg atcagcgcgt cacagtgacc aaaaacgtag
    31501 ttcgccagtt ggttaaattg ctcatcctgg aaggggccga cgctgtgacc aatcataaac
    31561 agcggctttt tcgccataaa cgtgcaaagc gcatgttcaa actgcggcac gccgtagaga
    31621 tcgacaaaaa acgatcctcc gacctgaata atggcgtcgt aacctgagag caggcgcacg
    31681 aagtcggtga atccctgggc gatggcgata ttacgcagct tgccagtgtc ggtgacgcgt
    31741 gagagcaata cctggtgttg atagcggcga cggaggactt ttttaacgcg cccgacaacg
    31801 cccgccgcgc tgttgtgttg tttcatttgc aggaacagag gatcgcccat taccgggcgg
    31861 ttgagtagcc aggaagaact gaccggatag cggctcatca cgtccacttc ggcttgtgga
    31921 ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg cgctgtcgcc acgattgccg
    31981 caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg atgccaagtg
    32041 aggctgtgtt tgttttgttc gttgccgctt tttgcctgat gcgacgctga cgcgtcttat
    32101 caggcctaca agacccgagc acagaaccgt aggccggata aggcgttcac gccgcatccg
    32161 gcattcagtg cctgatgcga cgctgacgcg tcttatcagg cctacaggtc ccgagcacag
    32221 aaccgtagga tggataaggc gttttacgcc gcatccggca accgttgtcg gaaccgaaaa
    32281 cagcaactca ccccgcccgt aaaagcattt tcattttttc gctgcgacaa aactgacgct
    32341 tcacttccac caccagcgga tggcgcgaca gcacaatcat caccacaaac gccagcacgc
    32401 ccgcggctat ttgcaccgcc agcagcatcc ccagcgccag ttgccctttc agcacaatgc
    32461 ccagcgcata actgaccacc agcgtcggca acgagagata aaacggcagc cacaaactca
    32521 ggatgtactg gcgataactg gaaccaagaa ccggtttaat catcacgaag taactcagaa
    32581 tggtgttgat aatctgcacc agcaggaaac cgagcgtgac gccgattgcg cccgccatct
    32641 gcccaccaat aacaatcgcc ggaataaaca gaaatgtttt gaatacgttg aatttaaagc
    32701 tgatatcgac ccgcgctttc gccatcagca gcgaaccaat cgggttaccc acggagcgca
    32761 gtagccccac cacacacaga aattgcagca ccggaataat gctgttccac ttctcaccaa
    32821 agaccagtgg tacaaagtta ttcgacacca ccattagccc gagcagcgcc ggaaagttga
    32881 taatccccac caccgacagc ggtttgtaga agttaatacg cagcttttcg gtgtcgtcct
    32941 gaattttggc aaacgccgga aacaacacac gggtgatgat tgggttcagc ttcatcggcg
    33001 gcacaacggc cacgttgtaa gccaggttgt agccccctgc cacgcccgcg ccgagaatac
    33061 gcgccagcac cagcgttgaa aggttggtat tgagatagtt gatgatgctg tccgccgtca
    33121 gccaggcacc aaagcgtaag ttcggtgcca ccgacgccag cgagaaatgc agaccggggc
    33181 gataaatttt gcggccaaag tagccaaaca gcagcgttct caccgcacta ttgaccagat
    33241 aaccgaggat cgcggtcatc gccagcggcc agaaatgggc gctaaccacc gtaaaagtga
    33301 agcccgccag taccgcgctg gtttcgatca tgccgatttt gttgaactcc agctcctttt
    33361 gcatcaacgc gcggaactgc tgcccctggg ggatcaccac aaacgccagc gacaatgttt
    33421 taatcaacgg tgccaggtcc gggttattca gcacgtcgcc gatgagatca ctcaacaaaa
    33481 acaccgccag acacaccaca agccccagcc cgacgttcag ccagtacagc gtggtgagtt
    33541 caaggtggct gatttctttt cgctgaataa tcgagttagc gataccgaag tcagaaagcg
    33601 tatctgccag cgcgatgatc accagcgaca cagtaagcaa accgaactgg tggttgtcga
    33661 taatccgcgc cagcacggtc atctgcacca gcccgaggcc gatgatgatc accgtggcaa
    33721 tcgccgacca cttcgcgccg ctgatggttt tttcacgtaa gctcatatca atatgccgct
    33781 ttgttaacga aacctttgaa caccgtcagg aaaacgattt tgatatcgaa ccagacgctc
    33841 cattcacgga tgtactcaag gtcgaactcg acgcgttttt ccattttctc cagcgtgtcg
    33901 gtttcgccgc gccagccgtt aatctgcgcc cagccggtaa tgcacggttt cactttatgg
    33961 cgcagcatgt agccttcaat gagctggcga tactgttcgt tatgcgctac tgcgtgcgga
    34021 cgtggaccga caatcgacat ccccccggtc agcacattga taaactgcgg taattcatcc
    34081 agcgaggtgc ggcgcagaaa gttccccact ttagtgacgc gcggatcgtt ctgcgtcgcc
    34141 tgggtcacca ctttgtcgtt ctccatcact ttcatggaac ggaacttcca cactttgatc
    34201 ggcttgccat ccatgccgta gcgagtctgg cggaaaataa ccggccctgg tgaactgagt
    34261 ttcaccgcca gcgcaataca gcacagcacc ggggagatca gcagcaagat cagcgtcgcc
    34321 agcacaatgt cttccgcacg ttttagcagg cggttaaccc cggaaagcgg cgtgtcatac
    34381 agcggcacca ccggaacgcc gttcatctct tcgagacgtg aatggagaat attgaaggta
    34441 aagacatcgg ggatcagcag caccgaacag gtggtgtccg ccagttgatg gaccagtttt
    34501 ttcactcgcg cgccgtcgca catttgcatc gcgatataga cgttatgaat cttacccgct
    34561 ttagcatcct cgaccagctg ttgcagattg cccgcccagt cgttagaaac accgcccggt
    34621 ttcgggtcgt ggtaaacgcc caccacttca aaccctaacc acggctgatt acggaagctc
    34681 tccatcagca tttgtccggc ggctaaatcc cccgccaccg cgaccatgcg cttgttatag
    34741 ccatgattac gcagccagcc cgccccaatg cgaatacatg aacggcaaac caccagtccg
    34801 atactggtca gcccatacca cgccagccag attttcagtt gcgtgtcgaa atcattgttg
    34861 aacgccacca gtccggcgct gaaaatcacg cttaaggtcc agttttgtag caacagggca
    34921 aattctgtcg ctgcccgaac accgcgccat gagcgataaa aatcggtgat gccgcccagc
    34981 atctggaaca ccaccagcgt aatcaacgcc accaacaggt gcatgtagag gaatgacagt
    35041 ccgctcactt cgcaaaccag ccatagtccg gcaaacatga tggtgatatc tgaaaagcgt
    35101 tgcaccatag agattaacga tgcattggtt ttcgctcgcg cttttttaga tttgtcatcg
    35161 ttgttcctgt tattagcccc ttacccgcag tggggtaagg gaagatccga cattactcgt
    35221 tcagcaacgt cagcagagtt cgcgttcgcg cttccatcag cggcacatca ccgcgtgatt
    35281 ccacattcaa ccgcaccacc ggttcggtgt tggaggagcg cagattaaag cgccagtcgg
    35341 caaaggtcat gctgatgcca tcggtgcgat ccaccgccag cgcatcacgg ctaaaatgct
    35401 gttccacgcg gttaatcgcc tcaacgggtt gcgccagttt gctgttgatc tcaccgcttg
    35461 ccggaaacgc cgccatccgg tcgcgcacca gttcgcccag cgtttttcct ttcaggcaca
    35521 ccagttcggc gaccagcagc cacgggatca tgccgctgtc gcagtaagcg aaatcacgga
    35581 agtaatggtg ggcgctcatt tcgccaccgt agatggcgtc ttccttgcgc atacgttctt
    35641 taataaaggc gtgtccggtt ttcgacatca ccgacgtgcc gccagcggca gtcaccacat
    35701 caacggtatt ccaggagaga cgtggatcgt ggatgatctt cgcgccggga tttttttcga
    35761 ggaacgcttc tgccagcagg ccgacaatgt agtagccttc gataaactgc cctttttcgt
    35821 caaacaggaa gcagcggtca aaatcgccat caaaggcaat gcccatatcc gcgccgtgtt
    35881 tgatgaccgc attgcgggtg tcgtcgcggc attccggcag tagcgggtta ggaataccgt
    35941 tggggaaatt gccgtccggc gtgttgtgca ctttgattaa ttccacgggt gcgccaaggg
    36001 ctttaaagcg ggcttcaatg gcgtccacca ccggacccgc tgcgccattc ccggagttaa
    36061 tcaccagctt gagcggcgtg aggtttttga cattgatata accgaacagg tgatcaacgt
    36121 aagcgtcacg caggttgatt tgctgatagc gaccgcgttt ggtttcatcg acgggaggaa
    36181 agtcgttggc ttcagccaga cgctggacgt cgcgcagtcc ggtatcaccg ctgatcgggc
    36241 gagccccttc gcgcaccagc ttcatgccgt tataatccat cggattatgg ctggcggtga
    36301 cttcaatgcc gccatccacg ccgagatgga acgtggcgaa atagatctct tcggtgccgg
    36361 acataccgat atccagcacg tcaacgcccg catcctgtaa accttttgcc agcgccagtt
    36421 ttaaggtttc gctggtgaga cggacatcac cgcctaacac aatggttttc ggtttgagaa
    36481 attcgccata ggcgcgacca atgcgccagg cgatatcttc attcagttct tcgcctaatt
    36541 tcccgcgaat atcataggct ttaaagcagg ttaatttttt catgtcgtta ccctttttca
    36601 ggcaatagtt ggccctgacc gaaacgacca attttgtttt tgtcgttatt tacatctcgg
    36661 tttcgcattc attgcctgat gcgacgctgc cgcgtcttat caggactaca aacccgaacc
    36721 gtaggtcgga taaggcgttc acgccgcatc cgacaaacag cgcctgatgc gacgtttaca
    36781 cccgtccgta gcgatccgcg aagcgcacca catcatcctc ttcgagataa gagccggagc
    36841 gcacttcaat taaatcaagc ggaattttcc ccggattttc caggcagtgc gtcgccccca
    36901 gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccatca atggtgactt
    36961 ttgccgttcc cgcgaccact acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca
    37021 aaccctcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag
    37081 agtcatattt gccccacgga cgatacactt cgcgatgcac ccgatgctca tggcgaccat
    37141 cggctttgat ctgctcgacc acttttttca catcctgtac cgcgttacgg tcggcaatca
    37201 gcaccgcatc tttggtctgc accactacca gatctttcac cccgacggtg gtgaccaggc
    37261 cagattcggc atacacatag ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa
    37321 cgttgccctc ggcggtgtgg gcgctgatct cccataatga agaccaggaa ccaacatcgc
    37381 tccagcccgc atccatcggc accacaacag catctgccgt acgttccatg accgcgtaat
    37441 ccaccgactc ttccgggcag gcgagaaacg cttcttcatc cacacgaata aaatcaagat
    37501 ccggatcgac ggagctcatc gctttttcac aggcatcgag aatatccggg cgatattttt
    37561 tcagttcttc gagatagcgt ccggcgcgga acaggaacat gccgctgttc cagtaatatt
    37621 cgccgcttgc cacataggcc tgggcggttt ccagattcgg tttttcgaca aactgcgcca
    37681 cttcaaaggc caccgtatcc tgctcacccg cagacacttc accgcgacga atatagccat
    37741 aaccggtttc tggtagatcc ggcacaatgc caaaggtcac cagcttgccc gcttcggcat
    37801 atggcatggc attacgcacg gcggcacgga acgcgtcttc atcggcaatt acatgatccg
    37861 ccgccagcac cagcattaac gggtcgttct ccgggctatg acgttttgcc gccagcgccg
    37921 ccagcgcaat ggccggtgca gtgttacgcc ctgcaggttc gagaataatg ttctcggtga
    37981 gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca ttgcaaatca
    38041 ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc agcatggtga
    38101 gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc ggccataagc
    38161 ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc ataattatcc
    38221 ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagcgtgc gttcggcata
    38281 ttcacgtgcc accgtgttgt gtttgggcag caggagcgcc tgactaatcc ccgccaccag
    38341 cgcctcgacc gattccggct ctacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg
    38401 ccccagttct gtgtgggctt ctgcggtaat caccgcgtta ccgcctaccg cctaccgcca
    38461 gaatattggt cagtttcgac ggcaatacgg catctgccgc gccgcatttt tgcaccacca
    38521 gatggcaatc gcccatcttc agcagtgcgg gtaaagcgtc atacgattgc agcgggaaaa
    38581 attgcatgtt gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc
    38641 cctgcccgac aatggcaaaa atcagcggtt catcgcgcag gcgatcggca gcttcaataa
    38701 cgttttccag tccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat
    38761 ccggtaggcc aagctggtta cgaagggcat caacatcggc gtctgcaaca tgctgaaaac
    38821 gggcgatttc cgaccagttg gggaagaaaa tgatgttatc cgccgccacg cctttttcga
    38881 tggctttatt catcatcgaa cgcgaaatcg tggagacgtt atcgacgtta tgcagtccgc
    38941 tacgttcgaa cgccgttgcc agctgtgcca ctttgccgcc tttgcctttt ccggcaaggc
    39001 ccagccccag catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag
    39061 agagtttcgc cagcaggcgc attcccggcg tgcaaaacag cgttggcacc acgccgataa
    39121 tgcgatccgg cttccagcga cgttgcgcca tcaacgggaa aaaactgctg acggcaaaac
    39181 cgccgagatg caacaggcgt ttcagggtgc tcggctgttt tggcacgtac agcgggcagc
    39241 gccacaccgt ggcggccccc tcttctcgtt tgtagcgcta ggcggaatag ttctcgccca
    39301 cctgccactg cgggtagtaa ggcggtgcgg taataacccg cacctcatga ccttgcgccg
    39361 ccagccattc caccatttct ccggtgtatt tgccgatgcc ggttaactcc ggcgagtagt
    39421 taatgccgta gaccagtatt ttcataatcc gggtactccg gcacgcttct cagcgagaaa
    39481 ataggcgcgg ctgttagcgt gaacattgtc actggcgagc agtgcgtccg gcgtcagcca
    39541 gcggtaatcg tcatgctgct catccggcag taacagttct tcttccgcta ctctgaagcg
    39601 aaaaccgagc accacatagt gagtggtgaa atccgtgcca gagaagttat cgtcataaaa
    39661 gtgctgccag acaccgtaaa actggcctgc tgttatcggc agacgcagcc ccagttccgc
    39721 catcgtcagc cgctcaaatg cggcttccag cgtttcgtct ttctgcacgc gccctcccgg
    39781 cacaaaccag taaccctgcg ccgggcggtt ggttcttttg ccaggcagaa actcgccgcg
    39841 actgttctcg acaataaagt cgagagagac aagcggtgtg gagcgcacta ccgtggcaaa
    39901 gtcttcctga cgtaaaaaca tcattacccc cgaaagcggt cttgattctc aaggaaccac
    39961 tggtaagtgc tggcaagccc cgcttccagt gagatttcgt gataccagcc aagctgatgc
    40021 aggcgcgtca catcaagcag tttgcgcggc gtaccatccg gtttactggc atcaaaaacc
    40081 acccgacctt tgtaacccac cactttggcg atggtttgcg ccagctcgcg gatagtgcag
    40141 tcaacgcccg tgccgacgtt aatgtgcgac agcatcggct gggtgttttc cagccagact
    40201 tcgtgcgcca gctccatcac atgaatgctc gccgccgcca tatcatcgac gtgcaggaat
    40261 tcacgcatcg gtgtaccgct gccccacacc accacgtccg gcgcattctg tgccgtcgcc
    40321 tcgtggaagc gacgcagcaa tgctgggatc acatgcgaat tactcgggtg gaagttgtca
    40381 tgcgggccat acaggttggt cggcatgacc gagcggtaat ctcgtccgta ctggcgattg
    40441 taagattcgc acagtttgat cccggcgatt ttggcaatgg cgtaaggctc gttggtcggc
    40501 tccagcgtgc tctgcaataa ctcgctttct gccatcggct gttttgccag tttcgggtag
    40561 atgcaggacg atccgagaaa cagcagtttg ttcacgtcgt tctgatgcgc ggcgtgaatg
    40621 atgttgctct caatcatcat gttctggtag atgaaatccg ccggataggt gttgttggca
    40681 acaatgccgc ccactttcgc cgccgccaga tagacctggt caatgcgttc gctggcaaag
    40741 aaatcatgca cggcgcggct gtccagcagg ttcagctcgt cgcgggtacg taataccagc
    40801 tccacatcac cgcgctgttc gagctgccgc ttgatggcag aaccgaccat cccacgatgg
    40861 ccagcgataa aaatgcgttg tttactcatg cttatgactc cagcgcgatc gccacgtcgt
    40921 agccgtgaga tttcagcaga gagtgttttt tcgccgcttc gaggtcatta gccaccattt
    40981 cagacaccat ctctctgagg gtgatttccg gtttccagcc cagtttttcg tgcgctttgg
    41041 tcgggtcgtc gagcagcgtt tcaacttctg ccggacggaa gtaacgcgga tcaacggcga
    41101 taatcacatc acccggttta acgcccggcg cgtcatgccc ggtgacggaa accacaatgc
    41161 ccttctcttc aacacccgtg ccttcaaagc gcagtttgat gcccagctgt gccgctgcca
    41221 tttccacgaa ctgacgtacg gagtactgaa cgccggtcac gataacgaaa tcttccggct
    41281 gttcctgttg cagcatcatc cactgcattt ttacgtagtc tttggcgtgg ccccagtcac
    41341 gcagggaatc catattgccg aggtacaggc acgactccag cccctgggcg atgttggcga
    41401 ttgcgcgggt gattttgcgg gtaacgaagg tttcgccgcg gcgcggggat tcatggttga
    41461 agagaatacc gttacaagcg tacatgccgt aggattcgca gtagttaacg gtgatccagt
    41521 aggcgtacag tttggcgacc gcatacggag atcgcgggta gaacggcgtg gtttctttct
    41581 gcggaatt
//
LOCUS       DAJTSL010000012        40625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTSL010000012 DAJTSL010000000
VERSION     DAJTSL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.012"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(265..1122)
                     /gene="murI"
                     /locus_tag="OO433_000583"
     CDS             complement(265..1122)
                     /gene="murI"
                     /locus_tag="OO433_000583"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6049175.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1067..2915)
                     /gene="btuB"
                     /locus_tag="OO433_000584"
                     /pseudo
     CDS             complement(1067..2915)
                     /gene="btuB"
                     /locus_tag="OO433_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     regulatory      complement(2969..3159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3273..4373
                     /gene="trmA"
                     /locus_tag="OO433_000585"
     CDS             3273..4373
                     /gene="trmA"
                     /locus_tag="OO433_000585"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6049176.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4413..4772)
                     /locus_tag="OO433_000586"
     CDS             complement(4413..4772)
                     /locus_tag="OO433_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6049177.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4772..5422)
                     /gene="fabR"
                     /locus_tag="OO433_000587"
     CDS             complement(4772..5422)
                     /gene="fabR"
                     /locus_tag="OO433_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6049178.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5743..7143
                     /gene="sthA"
                     /locus_tag="OO433_000588"
     CDS             5743..7143
                     /gene="sthA"
                     /locus_tag="OO433_000588"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6049179.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLVLDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7126..8043)
                     /gene="oxyR"
                     /locus_tag="OO433_000589"
     CDS             complement(7126..8043)
                     /gene="oxyR"
                     /locus_tag="OO433_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6049180.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8310..9683)
                     /gene="argH"
                     /locus_tag="OO433_000590"
     CDS             complement(8310..9683)
                     /gene="argH"
                     /locus_tag="OO433_000590"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6049181.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDAFDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9744..10520)
                     /gene="argB"
                     /locus_tag="OO433_000591"
     CDS             complement(9744..10520)
                     /gene="argB"
                     /locus_tag="OO433_000591"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6049182.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10528..11532)
                     /gene="argC"
                     /locus_tag="OO433_000592"
     CDS             complement(10528..11532)
                     /gene="argC"
                     /locus_tag="OO433_000592"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6049183.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPAINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11686..12837
                     /gene="argE"
                     /locus_tag="OO433_000593"
     CDS             11686..12837
                     /gene="argE"
                     /locus_tag="OO433_000593"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6049184.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDIDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVIERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            13189..15840
                     /gene="ppc"
                     /locus_tag="OO433_000594"
     CDS             13189..15840
                     /gene="ppc"
                     /locus_tag="OO433_000594"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6049185.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEAPPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYKSWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYL
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            16023..17756
                     /locus_tag="OO433_000595"
     CDS             16023..17756
                     /locus_tag="OO433_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6049186.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17971..18822
                     /locus_tag="OO433_000596"
     CDS             17971..18822
                     /locus_tag="OO433_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6049187.1"
                     /translation="MYHDISYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18809..19150)
                     /locus_tag="OO433_000597"
     CDS             complement(18809..19150)
                     /locus_tag="OO433_000597"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6049188.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19152..20030)
                     /locus_tag="OO433_000598"
     CDS             complement(19152..20030)
                     /locus_tag="OO433_000598"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6049189.1"
                     /translation="MTSSAGQRISCNVVETRLNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19996..22293)
                     /locus_tag="OO433_000599"
     CDS             complement(19996..22293)
                     /locus_tag="OO433_000599"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6049190.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAVLAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTPGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLWEAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22344..22664)
                     /locus_tag="OO433_000600"
     CDS             complement(22344..22664)
                     /locus_tag="OO433_000600"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6049191.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22679..23758)
                     /locus_tag="OO433_000601"
     CDS             complement(22679..23758)
                     /locus_tag="OO433_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6049192.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVVI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            24067..26568
                     /gene="ptsP"
                     /locus_tag="OO433_000602"
     CDS             24067..26568
                     /gene="ptsP"
                     /locus_tag="OO433_000602"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6049193.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSSILEAHRSLAGDTSLRQHLLTGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMTQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26580..27242
                     /gene="fsa"
                     /locus_tag="OO433_000603"
     CDS             26580..27242
                     /gene="fsa"
                     /locus_tag="OO433_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6049194.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27253..28356
                     /gene="gldA"
                     /locus_tag="OO433_000604"
     CDS             27253..28356
                     /gene="gldA"
                     /locus_tag="OO433_000604"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR6049195.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAAFSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYATLLVADQYGQRFLQEWE"
     gene            28630..29247
                     /locus_tag="OO433_000605"
     CDS             28630..29247
                     /locus_tag="OO433_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR6049196.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29274..30179)
                     /gene="yijE"
                     /locus_tag="OO433_000606"
     CDS             complement(29274..30179)
                     /gene="yijE"
                     /locus_tag="OO433_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6049197.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMHPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(30273..32453)
                     /gene="katG"
                     /locus_tag="OO433_000607"
     CDS             complement(30273..32453)
                     /gene="katG"
                     /locus_tag="OO433_000607"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6049198.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     SSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32782..33672)
                     /gene="metF"
                     /locus_tag="OO433_000608"
     CDS             complement(32782..33672)
                     /gene="metF"
                     /locus_tag="OO433_000608"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6049199.1"
                     /translation="MSFFHASQRDALNQRLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(34021..36453)
                     /gene="metL"
                     /locus_tag="OO433_000609"
     CDS             complement(34021..36453)
                     /gene="metL"
                     /locus_tag="OO433_000609"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6049200.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36456..37616)
                     /gene="metB"
                     /locus_tag="OO433_000610"
     CDS             complement(36456..37616)
                     /gene="metB"
                     /locus_tag="OO433_000610"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6049201.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWTNNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37893..38210
                     /gene="metJ"
                     /locus_tag="OO433_000611"
     CDS             37893..38210
                     /gene="metJ"
                     /locus_tag="OO433_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6049202.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38394..39002
                     /locus_tag="OO433_000612"
     CDS             38394..39002
                     /locus_tag="OO433_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6049203.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTVKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            39147..39822
                     /locus_tag="OO433_000613"
                     /pseudo
     CDS             39147..39822
                     /locus_tag="OO433_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(39994..40230)
                     /locus_tag="OO433_000614"
     CDS             complement(39994..40230)
                     /locus_tag="OO433_000614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049204.1"
                     /translation="MKAIQCCYFLALVSSNLMAGVMGNDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(40232..40625)
                     /locus_tag="OO433_000615"
                     /pseudo
     CDS             complement(40232..40625)
                     /locus_tag="OO433_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
BASE COUNT         9991 a        11161 c        10102 g         9371 t
ORIGIN      
        1 cccacacaga ttgtctgata aattgttaaa gagcagtgcc gcttcgcttt ttctcagcgg
       61 cgcggggtgt gcataatacg ctttcccgct acagagtcaa gcatttattt ttgctttctc
      121 tgtcggaatt ctcaggagaa ccccgctgac ccggcggcgt gtttgccgtt gttccgtgtc
      181 agtggtggcg cattataggg agttattccg gcctgacaag aggaaattta aaataatttt
      241 ctgaccgcgc aacattcaac caaatcagcc taaaactgcc agtttttcga gcgtttcgaa
      301 gccgtaacgc tgtaaaacgg gcaataattg ttcagcttct ggcgtcattg ccatacaaaa
      361 ggcaatattc gcatcggcag attttgcatc cggggcttca tgttctaaca accaggccgt
      421 tcggcgagca atcgctgcgc cagaatccac tagccgggtt ccctctggca gcacttgtaa
      481 cagttcttct tgtagtagag ggaaatgggt gcaacccaat acaacggtat ctggcggctc
      541 tttcattctt aaccacgggc gtaggatacg ttttagtgca tccagagaaa catcttcgcc
      601 atgtagcttc gcttcagcca actcaaccat ctctgccgag cccagcattt ctatctggca
      661 ttcattagcg aaacgcgcga tcagctcatg agtataagaa cgtttaactg ttccgcgggt
      721 tgccagtaat ccgacaatgc catttgccgt cagacgtgca gcaggtttaa tcgccggcac
      781 gacaccaaca accgggaagt cgaacttttc gcgtaatgca ggaagtgaaa cggtactggc
      841 agtgttgcaa gcgaccacag ccagcgcaag gggataacgt tcttgcaccg cggtgacaat
      901 tgccaccact cgctcaacaa taaacgcttc gcttttttcg ccatacggga aagcgacgtt
      961 atcgaaagca taaatgtaat ggagatccgg taagagatgc cggatctcgt catagaccga
     1021 caacccaccg acgccggagt caaacaccag cacggtggga cgtggttcag aaggtgtagc
     1081 tgccagacaa ggtgtattcc cgtcctgcag tttggtagcc atagactgtc tcataatctt
     1141 tgtcgaacag gttggctatt ttaccacgaa ctgtcaggtg agaggtgacc ggatacgcaa
     1201 ccgcaagatc ccacaagctc acaccgccca ttttaacggt ttgataagga taagatgagt
     1261 aatccttatc atagcgagtg cctaaatact gataagtaat accccagtcg aagtcataca
     1321 actgccagtc gagctggtat ttcacctgct gtttagcacg gcgtaacaac ggcgtgtcgg
     1381 taatcgcatt gcgcgcatcg acataatcat aactcacagt atgcgtcagt ggtccggtat
     1441 caaaattggc ggtcgcctcg acgcccttaa tccgcgcttt cccttcgttg taatatttca
     1501 gggtgtgatc atcataatcg atcaagtcac tgacatcgtt acgatatccg gaaatacgcc
     1561 agttcacccc agcggttaag ccttcaaacg cgccttccca ctgtttgctt ttctccgggt
     1621 ccagattcgg atttccgtag aagccataca gttgccccag atttggtgcc ttataagatg
     1681 tcccgtagga agcaatgaag cgataacctt cgatgaattc ccaaccggcg ctggtttgcc
     1741 aggttccatg acgaccaaac tgtgagttat cgtcactgcg tgctgcgcct tcaaaggtaa
     1801 aatcgccgac ttgttgcagc ccggtcagat agatgccggt attacgttga tcatatccat
     1861 cctcaacata acctgtaccc ggcgtcgtag tctgtttctg ccagtcgaca cccgcaccaa
     1921 tactaccgtg accaacgatg acattgtttg cccactggac ggtgtattgc ttcatctcat
     1981 cgagcgtcgc cgacgaatca taacgaccat aatggggatc gtagttgtaa tctttgctat
     2041 ggctatagct ggtaatgagt tgtgatttaa tcagttcgcc gttatagcgc agcccggcgt
     2101 cccaactttg gctatagagt ttacggatat cgagcaacgg tgaaccggga gaataatacg
     2161 cgtcataatt ggtacggtta tcatagccat agccgcgcac aaagccgctc caggcatcag
     2221 taaagttatg ctccagcgcg ccataaagcg ttttacttaa aaaaacatcg ttatctgtct
     2281 gcgcttgcgt tccggtatta ccataggcaa caacatcata accatgagta tgggcataat
     2341 cgcccaacag cgttacccgt gtcttatccc ccagttgttg ctgcgtagag acatcatagt
     2401 tctgataact attgcttccc caccctgctg aaatttccgt tccggggtca tcgcgcgtcg
     2461 tgatgatatt caccaccccg cctattgcat cggaaccata aacagcggag cgcggcccac
     2521 ggatatattc aacacgctgg acaagcgcac gcaataggga actggctaag gtcggcagaa
     2581 ccactcaccc ccgccagatt caggcgtacg ccatcaatta acaccaacac atgactggca
     2641 tttgtaccgc gaataaaaat agatgagagc tgacctgaac cgccgttttg ggtgatatcg
     2701 acgcccggaa gacggcgcag cacatcattg accgaggtcg actgccagcg gtcgatatcc
     2761 tgacgggtca caacggtggt tggtgcaagc acagtgctgc gcggctgttc aaaacggtta
     2821 gcagtaacga cgagagtatc cgggctggta tcctgtgccc aagcggaaaa ggctgtgaca
     2881 gaacacgccg tcagcagcga agctttttta atcattgtaa agcatccaca atagaagaag
     2941 gatgccgcag gtttcatcaa tattacgcga tgatgagacg ttggccggca ggtcttcggg
     3001 cttggagggg tatctaagat actaagagat gatgacttcc caccggatgg cagtgtccgc
     3061 ataacgcaat catcgcacct ttccttaccg ctgcgcgtca gctccagatt cgcactggat
     3121 tccctattaa ctcacaggac cggcaagtgg atgctacagg ttgtaacaag ttactgtcca
     3181 gacgtagctc acaaatagga attcatcaag atctggacat ctgatgagca atccctacaa
     3241 tcgccgcgta ctttaatttt tcaggataca tcatgacccc cgaacacctt ccaacagaac
     3301 agtatgaagc gcagttagcc gaaaaagtgg tacgtttgca aagtatgatg gcaccgtttt
     3361 ctgacctggt tccggaagtg tttcgctcgc cggtcagtca ttaccggatg cgtgcggagt
     3421 tccgcatctg gcacgatggc gatgacctgt atcacatcat tttcgatcaa caaaccaaaa
     3481 gccgtatccg cgtggatagc ttccccgccg ccagtgaact tatcaaccag ttgatgacgg
     3541 cgatgattgc gggtgtgcgt aataatcccg ttctgcgcca caagttgttc cagattgatt
     3601 acctcaccac gctgagtaat caggcggtgg tttccctgct ataccataag aagctggatg
     3661 atgagtggcg tcaggaagcg gaggccctgc gcgatgcact gcgcgcgcag aatctgaatg
     3721 tgcatctgat tggtcgggca acgaaaacca aaatcgagct ggatcaagat tacatcgacg
     3781 agcgtctgcc ggtcgcaggg aaagagatga tctaccgtca ggtagaaaac agctttaccc
     3841 agccgaacgc ggcgatgaat attcagatgc tggaatgggc gctggacgta accaaaggct
     3901 caaaaggcga tttactggag ctgtactgcg gcaacggtaa cttttcatta gcgctggcgc
     3961 gcaattttga tcgggtatta gccaccgaaa tcgctaagcc gtcggttgct gctgcgcaat
     4021 acaacatcgc agctaaccat attgataacg tacaaattat tcgtatggcg gcagaagaat
     4081 ttactcaggc gatgaatggt gtgcgcgagt ttaaccgcct gcaagggatc gacttaaaga
     4141 gttatcagtg cgaaaccatt tttgtcgacc caccgcgcag cggtctggac agtgaaaccg
     4201 agaaaatggt gcaggcgtat ccgtgtattt tgtacatctc ctgtaacccg gaaacgttat
     4261 gcaagaatct ggaaacatta agccagacgc acaaggtcga acgtctggct ctgtttgatc
     4321 agttccccta cacgcaccat atggagtgcg gcgtattact gaccgcgaag taaaacccat
     4381 gccggatgcg ccagcatccg gcataatacc gattactcag caacttcctg cttacggtta
     4441 cgcatcttcg cgccaatcca gaacaccata atgacggaga gtactgccgg gaagaagtta
     4501 gagccgatat ccggatattc cgcacgaact accgtgctgt acagcagtac gccgagaata
     4561 aaacaggcag ctgccagacc cggcaagcct accggcatag tgcgattaag ataacgttga
     4621 tgcaggcagt aaaccgtcag caccagggaa ataatcggga atacagaaaa tggcacaatg
     4681 gagctaaaca gcgctgcgaa agtaccatta atcgataagc cagcaactaa cgccagcagc
     4741 agcgtacctc tatcttgatt tgcttgtttc attactcgtc cttcacattt cccggaataa
     4801 ttgcggtttt ctcttgttca cggcgatacc agtaataagc ccctttcgaa atcattcgca
     4861 gttgcagtac cagtcgctct tctaattgcc gacgttgttc gacgccgacg tccaacgcct
     4921 cggcacccgc actgaagaca attgtcacca ttgcttcggc ttgcgcttca gtaaacgcac
     4981 gcggcatatg gttttcgagt tccagatagt ccgcaagttc cgcaatgaag tgctgaattt
     5041 cacgcgcaac ggcggcacga aacgcagcgg aggtgccgga gcgttcccgc aataataacc
     5101 ggaaggcgtt aggattatta ccgatgaact ccataaatgt ggagaccgag gtgcggatca
     5161 cactcccgcc tttggcgata cgctgacgcg cctggcgcat gagttggcgt agcattaaac
     5221 cgctctcatc aaccatggtc agacccagtt cgtctacgtc gcggaaatgc cgataaaaag
     5281 aggtgggagc aatgcccgct tcacgcgcca cttcacgcaa actcaggctg gcgaagctgc
     5341 gttcagcact taattggcta aatgcggctt ccaccagcga acggcgggtt ttttcttttt
     5401 gttgcgctct tatgcccatc acgatgtctg aatccttgcc aaaagtactg ctggcactat
     5461 accagagaat gaacatactg gattgctcca gtattgtgaa tgaacggtaa cgcaataaaa
     5521 catatttact ggccggttct tttacgtaca gcggaaacct gccgcttaaa cggagagtat
     5581 cgtcgataaa aatccaataa aacgtcaggg caaaagtaag aaacagacaa agcaaaggcc
     5641 gctcaggata tagccagata aatgacgggg atcaattggc ttacccgcga taaaatgtta
     5701 ccattctgtt gcttttatgt ataagaacag gtaagcccta ccatgccaca ttcctacgat
     5761 tacgatgcca tagtaatagg ttccggcccc ggcggcgaag gcgctgcaat gggcctggtt
     5821 aagcaaggtg cgcgcgtcgc agttatcgag cgttatcaaa atgttggcgg cggttgcacc
     5881 cactggggca ccatcccgtc gaaagctctc cgtcacgccg tcagccgcat tatagaattc
     5941 aatcaaaacc cactatacag cgaccattcc cgactgctcc gctcttcttt tgccgatatc
     6001 cttaaccatg ccgataacgt gattaatcaa caaacgcgca tgcgtcaggg attttacgaa
     6061 cgtaatcact gtgaaatatt gcaggggaac gctcgctttg ttgacgagca tacgttggtg
     6121 ctggattgcc cggacggcag cgtcgaaaca ctaaccgctg aaaaatttgt tattgcctgc
     6181 ggctctcgtc catatcatcc aacagatgtt gatttcaccc atccacgcat ttacgacagc
     6241 gactcaattc ttagcatgca ccacgaaccg cgccatgtac ttatctatgg tgctggagtg
     6301 atcggctgtg aatatgcgtc gatcttccgc ggtatggatg taaaagtgga tctgatcaac
     6361 acccgcgatc gcctgctggc atttctcgat caagagatgt cagattctct ctcctatcac
     6421 ttctggaaca gtggcgtagt gattcgtcac aacgaagagt acgagaagat cgaaggctgt
     6481 gacgatggtg tgatcatgca tttgaagtcg ggtaaaaaac tgaaagctga ctgcctgctc
     6541 tatgccaacg gtcgcaccgg taataccgat tcgctggcgt tacagaacat tgggctggaa
     6601 actgacagcc gcggacagct gaaggtcaac agcatgtatc agaccgcaca gccgcacgtt
     6661 tacgcggtgg gcgacgtgat tggttatccg agcctggcat cagcggccta tgaccagggg
     6721 cgcattgccg cgcaggcgct ggtaaaaggc gaagccaccg cacatctgat tgaagatatc
     6781 cctaccggca tttacaccat cccggaaatc agctctgtgg gcaaaaccga acagcagctg
     6841 accgcaatga aagtgccata tgaagtgggc cgcgcccagt ttaaacatct ggcacgcgca
     6901 caaatcgtcg gcatgaacgt gggcacgctg aaaattttgt tccatcggga aacaaaagag
     6961 attctgggta ttcactgctt tggcgagcgc gctgccgaaa ttattcatat cggtcaggcg
     7021 attatggaac agaaaggtgg cggcaacact attgagtact tcgtcaacac cacctttaac
     7081 tacccgacga tggcagaagc ctatcgggta gctgcgctaa atggcttaaa ccgcctgttt
     7141 taacacttta tcgaaatggc catccattct tgcgcggatg gcctctgcca gctgctcata
     7201 gcggctgcgc agcggtgagc caggacgata aaccaggcca atcgtgcggc gaggttccgg
     7261 cttaatacac ggcaaataaa caaccccatc gcgtttgcgc tccggcggca cagccagcgc
     7321 aggcagtaaa gtgatcccgc tacctgccgc caccatgtta cgcagtgtct ccaggctggt
     7381 cgcgcggaag tgtgtatctt catccgcccc ggcttcaaag cagaaaccca ttgcctgatc
     7441 gcgcaaacag tgaccatctt ccagcatcag cagtttttcc cctgccagat cggccatcgg
     7501 tacgcattcg cggttcgccc acgggtgatc ttcatagata gccagcaaca ttggctcatc
     7561 aaacaacggc acttcaatga atgcttcgct ctctttcacc agcgcgagga tcacgcaatc
     7621 gagtttgccg ctgtccagtt gcgccagtaa ctggtgggtc tgtgcttcat gcagatacat
     7681 ttccagcttt ggaaatgtct ggtgcagcat cgggataata tgcggcagca ggtacggtcc
     7741 aaccgtagga atcaaaccaa tatgcagcgg tccggacatc gtctcgccct gctggcttgc
     7801 catctcttta aggactttca cctcacgcag cacggtacgc gcctgatcca ccagcagcat
     7861 tcccgcctgg gtgaacaaca ctttacggct ggtccgctcc agcaacatca cgcccagctc
     7921 atcttccagc ttacgaattt ggccgctaag cgtcggctgg ctaacgtggc aggaatctgc
     7981 cgcacgccga aaatggcggt gttcagccaa tgccaccagg tactcaagat cacgaatatt
     8041 cattatccat cctccatcgc cacgatagtt catggcgata ggtagaatag caatgaacga
     8101 ttatccctat caagcattct gactgataat tgctcacaga aacggagcgg cacctctttt
     8161 aacccttgaa gtcactgccc gtttcgagag tttctcaact cgaataacta aagccaacgt
     8221 gaacttttgc ggatctccag gatccgcttt tttttgccat aaaaaagccc ggcgataagc
     8281 caggctcaaa tttgtacata taaatgttct taccccaacc gcgcctgcgc aaaagcaatc
     8341 gcttgggcca cctgctgcgg tgagacgccg ccttttgccg cacgcttgtc gaggcacgat
     8401 tgtagcgaca gaatcggata gacatcttcg ccaatcacgt ggctgaattt ctgcaactcg
     8461 tcgagcggca gatcttccag cggtttgccc tgacgaatgg cttccaccac cgcttcaccc
     8521 acgatatggt gcgcctcgcg gaaaggtacg cctttcgcca ccaggtaatc cgccagttcg
     8581 gtggcgttgg cgtaaccctg ctgagccgct tcctggcaac gtggacgttt cacctgaatg
     8641 ccgtccagca ccagcgccgc catatgcagg cagtccagcc aggtatcgaa cgcgtcgaac
     8701 agaccttctt tgtcttcctg catatctttg ttgtaagcca gcggcaaacc tttcagcgtc
     8761 atcatcatgc cggttaacgc accctgcacc cgaccgcatt taccgcgaat cagctccagc
     8821 gcatccgggt ttttcttctg cggcattaat gatgaaccgg aagtcacgcg gtcagaaagc
     8881 tccacaaacc ccgcttcgcc ggtgttaaag aaaatcagat cttcagcaaa acgcgacaga
     8941 tgcaccatgc cgatagcggc agccgacagc agttccaaca catggtcacg gtcagaaacg
     9001 ctgtccagac tgttacgggt cgccgaagca aagcccagcc agcctgctaa ctgttcacgg
     9061 tcgatttcat aggccgttcc cgccagcgcg ccacagccta atgggctgac atccagacgc
     9121 ttaagcgcat cctgcaaacg gctttcatca cgcgccagca tctcaacata ggccaggcac
     9181 cagtgcgcga acgtcaccgg ctgggcgcgt tgcaggtgag tgtaacctgg cattaccgcg
     9241 tcctgattgt tttgtgcggt ttccaccagc gccgattgca gctgccggtt agccgtcagt
     9301 aactcgctaa cggtatcttt gcaccacagt ttcaggtcag tcgctacctg atcattacgg
     9361 ctacgcccgg tatgcagctt tttgcctaac tggcccactt tgtcgatcag tttgccttcc
     9421 acccagctat ggatatcttc ggcgtcgctt tcaaggattt gttgtggcct ggcgcgaaca
     9481 tcttccagca acacgttcag cgcctcttcc agttgcgcct gctcttctgc ggttaacacg
     9541 ccgaccgtta ccagggcttt ggaccaggcc acagagccaa caatatcctg ctccgccaga
     9601 cggtaatcaa agcgcagtga gtcgttgaat tgtttgaacc gttgatctgc tgcctgggta
     9661 aaacgcccgc cccaaagtgc cataactctg tttccttatt ttgaaattca atgccggaaa
     9721 gctgcgcctc cggctaacaa aacttaagct aaaatccgcg tacccatcgg cataccgtta
     9781 aacagtgccg gaagctgctc cgcatgacgc caggaggcga tatctaccgg acggcccagc
     9841 gtgcgggccg catccagcgc cgcgttcact ttcactatca tgccgtcagt aataatgccc
     9901 tgctcaatca gttgttctgc tttcgcggcg gtcatttcgg caatgcgttg ccctttgccg
     9961 tcgagaatgc cgctgacgtc ggagagcaaa atcagatccg cgcccagcgt tgccgccagc
    10021 gccgttgccg cctggtcggc attgacgttc atcagttgcc cttcgtctgt tacgccaatg
    10081 gagctgacca ccggcagata accgttctcc agcaaggagt tgataagctt aggcgaacct
    10141 ggctgcgcca gtccaacatg acctaactct tcatcaagct gggtcacttt gacgctgtcg
    10201 ccatcaccga gaaacaaacc tacagccgca atctgatgtt tcttcgccca tgccaacagg
    10261 gttttatttg ccgttcccgc cagtgctccg gtgataatgt ctatctgatc agcaggcgtc
    10321 acccgcaggc cgtttttctt tttcaccggc agattcagcc ctttcatcag ctcatccacc
    10381 acgcaaccgc cgccgtgcac aatcaccagc ggacgctgat gtgactcacg ataattcacc
    10441 agtgcgctaa acagacgttc cagcgcctct tcactatcca gcagtacgcc gcccagtttg
    10501 ataattaatg gattcatcat tgcaccctta aataagagac tgcgtttccg catagccgaa
    10561 acgaatattg gcgcactgta ccgcttgtgc cgccgcgcct ttcagtaagt tgtcttcggt
    10621 cgccacaata atcagatgct cgccctgaac ggcaaacccg atatcgcaaa atggcagccc
    10681 aacgacattt ttcagcgccg gaacgccttt gtcatacagc cgcaccagcg gtttatgggc
    10741 atacgcctgc tgtaacactt gcgcgacttg cgcctgggtc acacccgatt tcaggcggca
    10801 ggtaatggtt tcgagaatgc cgcgcgggaa attgcccaga tgtggggtga agataacgtc
    10861 agcaccgagg tgtgtggcga tctctggttg atggcgatga gtaaagacgc catacggttg
    10921 caggctaact tcacaaaaac tatttgaaat cgctgcctta cgtcctgcac cgctcacgcc
    10981 gctggtggcg ttaatcgccg gccactgatt gaggtcaaga agatcggcat caatcaacgg
    11041 tttcagcgcc agctgtgccg ccgtcggata acagcctggc accgcaatca aattcgcttc
    11101 ttttaattta ttaccgcacc actccgccag accgtaggct gcctgttcca gcagttccgg
    11161 gtattgatgg gtaaagccgt aatatttttc atagaaggtg gcgtcgttaa cacgaaacgc
    11221 gccggaaagg tcgaacacca cgcagcccgc ttcaagaaac tgcggcgcta aatcgtggct
    11281 aacttcatgg gcggtggcga gaaacactac gtccacccct gggctaaact cgctgatatc
    11341 cgacatcggc tgcaacggca gctcaacgat gccttttagc tgcggatgca aatcggagat
    11401 taactttccc gcatcattgc tttgcgctga aacagtcaaa gcggttatgt tcatatgcgg
    11461 atggcgattt acataggtca ctagctctgc gccagcgtag ccgctggcac ccacaatcag
    11521 cgtattcaac atcggggcta ttcaccttct tatgtctggt tgccaggtta aacgtaaaac
    11581 attcacctta cggctggtgg gttttattac gctcaacgtt agtgtatttt tattcataaa
    11641 tactgcatga atattgatac tatcatgacc agaggtgtgt caacaatgaa aaacaaatta
    11701 ccgccattta tcgagattta ccgcgctctg attgccacac cttcaataag cgctacggaa
    11761 gaggcactcg atcaaagcaa tgcagattta atcactctgc tggcggactg gtttaaagat
    11821 ttgggcttca atgtggaagt gcagcctgtt ccaggaactc gcaacaaatt caatatgctg
    11881 gcaagtatcg gacagggggc tggcggcttg ttgctggcgg ggcataccga tacggtgcca
    11941 tttgatgacg gtcgctggac gcgcgatccg tttacactga cggagcatga cggcaagctt
    12001 tacggcttag gcaccgccga catgaaaggc ttttttgcgt ttatccttga tgcgctacgc
    12061 gatatcgacg tcacgaaact gaaaaaaccg ctctacattc tggcgactgc tgatgaagaa
    12121 accagtatgg ccggagcgcg ttattttgcc gaaactaccg ccctgcgccc ggattgcgcc
    12181 atcattggcg aaccgacgtc actacaaccg gtacgcgcac ataaaggtca tatctctaac
    12241 gccatccgta ttcagggtca gtcggggcac tccagcgatc cagcacgcgg agttaacgct
    12301 atcgaactaa tgcacgacgc catcgggcat attttgcaat tgcgcgataa cctgaaagaa
    12361 cgttatcact acgaagcatt taccgtgcca taccctacgc tcaacctcgg gcatattcac
    12421 ggtggcgacg cttctaaccg tatttgcgct tgctgtgagt tgcatatgga tattcgtccg
    12481 ctgcctggca tgacactcaa tgaacttaat ggtttgctca acgatgcatt ggctccggtg
    12541 atcgaacgct ggccgggtcg tctgacggtc gacgagctgc atccgccgat ccctggctat
    12601 gaatgcccac cgaatcatca actggttgaa gtggttgaga aattgctcgg agcaaaaacc
    12661 gaagtggtga attactgtac cgaagcgccg tttattcaaa cgttatgccc gacgctggtg
    12721 ctcggtccgg gttccatcaa tcaagctcat cagcctgatg aatatctgga gacgcggttt
    12781 attaaaccca cccgtgagtt gattatccag gtaattcacc atttttgctg gcattaaaac
    12841 gtaggccgga taaggcgctc gcgccgcatc cggcaccgtt gccaaactcc ggtgccgcaa
    12901 taatgtcgga tgcgatgctt gcgcatctta tccgacctac acctttggtg ttatgtatgg
    12961 cgatttttta acatttccat aagttacgct tatttaaagc gtcgtgaatt taatggcgta
    13021 aattcctgct atttattcgt ttgctgaagc gatttcgcag catttgacgt caccgctttt
    13081 acgtggcttt ataaaagacg acgaaaagca aagcccgagc atattcgcgc caatgcgacg
    13141 tgaaggatac agggctatca aacgataaga tggggtgtct ggggtaatat gaacgaacaa
    13201 tattccgcat tgcgtagtaa tgtcagtatg ctcggcaaag tgctgggaga aaccatcaag
    13261 gatgcgttgg gagaacacat tcttgaacgc gtagaaacta tccgtaagtt gtccaaatct
    13321 tcacgcgctg gcaatgatgc taaccgccag gagttactca ccaccttaca aaatttgtcg
    13381 aacgacgagc tgctgcccgt tgcgcgtgcg tttagtcagt tcctgaacct ggccaacacc
    13441 gccgagcaat accacagcat ttcgccgaaa ggcgaagctg ccagcaaccc ggaagtgatc
    13501 gcccgcaccc tgcgtaaact gaaaaaccag ccggaactga gcgaagacac catcaaaaaa
    13561 gcagtggaat cgctgtcgct ggaactggtc ctcacggctc accctaccga aattacccgt
    13621 cgtacactga tccacaaaat ggtggaagtg aacgcctgct taaaacagct cgataacaaa
    13681 gatatcgctg actacgaaca caaccagctg atgcgtcgcc tgcgccagtt aatcgcccag
    13741 tcatggcata ccgatgaaat ccgtaagctg cgtccaagcc cggtagatga agccaaatgg
    13801 ggctttgccg tagtggaaaa cagcctgtgg caaggcgtac caaattacct gcgcgaactg
    13861 aacgaacaac tggaagagaa cctcggctac aaactgcccg tcgaatttgt tccggtccgt
    13921 tttacttcgt ggatgggcgg cgaccgcgac ggcaacccga acgtcactgc cgatatcacc
    13981 cgccacgtcc tgctactcag ccgctggaaa gccaccgatt tgttcctgaa agatattcag
    14041 gtgctggttt ctgaactgtc gatggttgaa gcgccccctg aactgctggc gctggttggc
    14101 gaagaaggtg ccgcagaacc gtatcgctat ctgatgaaaa acctgcgttc tcgcctgatg
    14161 gcgacacagg catggctgga agcgcgcctg aaaggcgaag aactgccaaa accagaaggc
    14221 ctgctgacac aaaacgaaga actgtgggaa ccgctctacg cttgctacca gtcacttcag
    14281 gcgtgtggca tgggtattat cgccaacggc gatctgctcg acaccctgcg ccgcgtgaaa
    14341 tgtttcggcg taccgctggt ccgtattgat atccgtcagg agagcacccg ccataccgaa
    14401 gcgctgggcg agctgacccg ctacctcggt atcggcgact acaaaagctg gtcagaggcc
    14461 gacaaacagg cgttcctgat ccgcgaactg aactccaaac gtccgcttct gccgcgcaac
    14521 tggcaaccaa gcgccgaaac gcgcgaagtg ctcgatacct gccaggtgat tgccgaagcg
    14581 ccgcaaggct ccattgccgc ctacgtgatc tcgatggcga aaacgccgtc cgacgtactg
    14641 gctgtccacc tgctgctgaa agaagcgggt atcgggtttg cgatgccggt tgctccgctg
    14701 tttgaaaccc tcgatgacct gaacaacgcc aacgatgtca tgacccagct gctcaatatc
    14761 gactggtatc gcggcctgat tcagggcaaa cagatggtga tgattggcta ttccgactca
    14821 gcaaaagatg cgggcgtgat ggcagcgtcc tgggcgcaat atcaggcaca ggatgcatta
    14881 atcaaaacct gcgaaaaagc gggtattgag ctgacgttgt tccacggtcg cggcggttcc
    14941 attggtcgcg gcggtgcacc tgcccatgcg gcactgctgt cacaaccgcc aggaagcctg
    15001 aaaggcggcc tgcgcgtaac cgaacagggc gagatgatcc gctttaaata tggtctgcca
    15061 gaaatcaccg tcagcagcct gtcgctttat accggggcga ttctggaagc caacctgctg
    15121 ccaccgccgg agccgaaaga gagctggcgt cgcattatgg atgaactgtc agtcatctcc
    15181 tgcgatgtct accgcggcta cgtacgtgaa aacaaagatt ttgtgcctta cttgcgctcc
    15241 gctacgccgg aacaagaact gggcaaactg ccgttgggtt cacgtccggc gaaacgtcgc
    15301 ccaaccggcg gcgtcgagtc actacgcgcc attccgtgga tcttcgcctg gacgcaaaac
    15361 cgtctgatgc tccccgcctg gctgggtgca ggtacggcgc tgcaaaaagt ggtcgaagac
    15421 ggcaaacaga gcgagctgga ggctatgtgc cgcgattggc cattcttctc gacgcgtctc
    15481 ggcatgctgg agatggtctt cgccaaagca gacctgtggc tggcggaata ctatgaccaa
    15541 cgcctggtag acaaagcact gtggccgtta ggtaaagagt tacgcaatct gcaagaagaa
    15601 gacatcaaag tggtgctggc gattgccaac gattcccatc tgatggccga tctgccgtgg
    15661 attgcagagt ctattcagct acggaatatt tacaccgacc cgctgaacgt attgcaggcc
    15721 gagttgctgc accgctcccg ccaggcagaa aaagaaggcc aggaaccgga tcctcgcgtc
    15781 gaacaagcgt taatggtcac tattgccggg attgcggcag gtatgcgtaa taccggctaa
    15841 tcttcctctt ctgcaaaccc tcgtgctttt gcgcgagggt tttctgaaat acttctgttc
    15901 taacaccctc gttttcaata tatttctgtc tgcattttat tcaaattctg aatatacctt
    15961 cagatatcct taaggaattg tcgttacatt cggcgatatt tttttcaaga caggttctta
    16021 ctatgcattc cacagaagtc caggctaaac ccctttttag ctggaaagcc ctgggttggg
    16081 cactgctcta cttttggttt ttctctactc tgctacaggc cattatttac atcagtggtt
    16141 atagtggcac caacggcatt cgcgactcgc tgttattcag ctcgctgtgg ttgatcccgg
    16201 tattcctctt tccgaagcgg attaaaatta ttgccgcagt aatcggcgtg gtgctatggg
    16261 cggcctctct ggcggcgcta tgctactacg tcatctacgg gcaggagttc tcgcagagcg
    16321 ttctgtttgt gatgttcgaa accaacacca acgaagccag cgagtattta agccagtatt
    16381 tcagcctgaa aattgtgctt atcgcgctgg cctatacggc ggtggcagtt ctgctgtgga
    16441 cacgcctgcg cccggtctat attccaaagc cgtggcgtta tgttgtctct tttgccctgc
    16501 tttatggctt gattctgcat ccgatcgcca tgaatacgtt tatcaaaaac aagccgtttg
    16561 agaaaacgtt ggataacctg gcctcgcgta tggagcctgc cgcaccgtgg caattcctga
    16621 ccggctatta tcagtatcgt cagcaactaa actcgctaac aaagttactg aatgaaaata
    16681 atgccttgcc gccactggct aatttcaaag atgaatcggg taacgaaccg cgcactttag
    16741 tgctggtgat tggcgagtcg acccagcgcg gacgcatgag tctgtacggt tatccgcgtg
    16801 aaaccacgcc ggagctggat gcgctgcata aaaccgatcc gaatctgacc gtgtttaata
    16861 acgtagttac gtctcgtccg tacaccattg aaatcctgca acaggcgctg acctttgcca
    16921 atgaaaagaa cccggatctg tatctgacgc agccgtcgct gatgaacatg atgaaacagg
    16981 cgggttataa aaccttctgg atcaccaacc agcagacgat gaccgcccgc aataccatgc
    17041 tgacggtatt ttcgcgccag accgacaagc agtactacat gaaccagcaa cgtacgcaga
    17101 gtgcgcgtga atacgacacc aacgtgctga agccgttcca ggatgtgctg aatgaccctg
    17161 cgccgaagaa actgatcatc gttcatctgc tgggtacgca tatcaaatac aaataccgct
    17221 acccggaaaa tcagggcaag tttgatggca ataccgatca tgttccgcca ggattaagcg
    17281 cagaagagct ggaatcatat aacgattatg acaacgctaa cttgtataac gatcatgtgg
    17341 ttgccagcct gattaaagac tttaaagcgg cagacccgaa cggattcctg gtttacttct
    17401 ctgaccacgg tgaagaggtt tacgacacgc cgccgcacaa aactcagggg cgtaacgaag
    17461 acaacccgac gcgccacatg tacaccattc cgttcctgct gtggacgtcg gaaaaatggc
    17521 aagcgactca tccccgtgat ttctcacagg atgtcgatcg taaatacagc ctggcggaac
    17581 tgatccacac ctggtcagat ttggcgggct tatcttacga cggttacgat ccaacccgtt
    17641 cagtggtgaa tccgcagttc aaagaaacta cccgctggat tggtaacccg tacaagaaaa
    17701 acgcgctgat cgattacgac actctgccgt atggcgacca ggtaggtaat cagtaattat
    17761 cagccaggcg ctatttgccg gatgcgacgc ttgacgcgtc ttatccggcc tacagatcgc
    17821 ggcggtaggc tggataaggc gtttacgcca catccggcaa tacaactgcc gcaatgttta
    17881 catcgaactc ccctcaggcc acacttttca ggtggccttt tttaataatg ccagttactg
    17941 ttttcccgtc tacataccta agggaataac atgtatcacg acatcagcta tctgctctcc
    18001 cgcctgatca acggcccgct gtccctgcgc cagatttact ttgccagcag taacggcccg
    18061 gtgcccgatc ttgcgtatca ggtcgatttt ccacggctgg aaattgtgct ggaaggtgag
    18121 tttgttgata ccggcgctgg agcaacgtta gttcccggcg atgtgctgta cgttgccgct
    18181 ggtggctgga atttcccaca atggaaaaca cccgctacca cctttagcgt gctgtttggc
    18241 aaacagcaac tcggcttcag cgtcgtgcaa tgggatggca aacaatatca aaatctggcg
    18301 aagcaacacg tcgcccgacg cggcccacgt ataggttctt ttctgctaca aacgctcaat
    18361 gaaatgcaaa tgcagccgca ggagcagcaa acggcaaggc ttatcgtagc cagcctgctt
    18421 agccactgcc gcgatttgct cggcagccaa atccagaccg cctcacgcag ccaggcacta
    18481 tttgaagcta ttcgcgatta tatcgacgaa cgctatgcct ccgcgcttac ccgcgaatct
    18541 gtcgcacagg cattctatat ttcaccaaac tatctctcgc acctgtttca aaaaacgggg
    18601 gccattggtt ttaacgaata cctgaatcac acgcgactgg agcacgctaa gacgttattg
    18661 aaaggctatg acctgaaggt aaaagaagtg gcgcacgcct gcggctttgt cgacagcaac
    18721 tacttctgcc gtctgtttcg taaaaacacc gaacgctcgc cgtcggaata tcgccgacag
    18781 taccacagcc aactgacaga aaaaccgact actccagaat aagatgggtt tgctgcggcg
    18841 cagaaagcac tttgcgcacc gcgctcatta cccgctgcgg ctcacgcagg aatgcgtaga
    18901 tgctgcattg cacatagcgg caatgttcaa atcgctcggc accggcaagc tcgatatccg
    18961 taatcaacag agcaacatcc gcccgacgga tatcctcgtc cgctaaacga ttctccgttc
    19021 ccagcgcgcc ctgagtttca atgctgacgc cccacttctc taactggcac aacttttcca
    19081 gccgttccgc cgccatataa gtatgcgcca cgccgcttac gcaggcggtt actgccacca
    19141 ggtatgccat tttaacctcc cacggtaacc tgaaatccgg cccgttctgc catttcgcgc
    19201 atcgttgcca catcggctga tgacggcgca gcaacatcct tcatcgacca tgttttcccc
    19261 agcaggcggt atttcggctc gccgtactga tgaaacggca acagatggat ctgcctgata
    19321 ttcagcggga tcagcacgtc cagcgcctgc tgcatattct cccggctgag tgtgaaaccg
    19381 gggatcagcg gcaaacgcgg gatcacgttg acaccctcac tcaccagcaa acgcaggttc
    19441 tccagtacgc gtggcaggtt catcttcacc acatcccgcg cctgagtcgc gtccataatt
    19501 tttaaatcga acaacacttc atcgcacaat ttcgccagcg gtaacagctt gctggctggt
    19561 gcgtctccgg cagtttcaat ggcacatgag acaccccaca gccgcagtcg ctgtaaaaaa
    19621 cgggtagcaa actccgcctg cattaacact tcgccgccag aaagcgtgac gccgccgccg
    19681 gacgtgcgaa aaaaaatgtc atctttcatc acttcccgtt ccagagcgtc aaggctgata
    19741 tcgcgaccaa tccgttcaaa cgccccggag gggcattcat ccgcatcacg caaacatttc
    19801 gcacagtgca gacatttcgc ctctctgcgt accgtctgga ttttgccgga gatcgactcc
    19861 ggattagcac accacgggca aagatgcgga cagcctttaa aaaagaccac cgtacgaatg
    19921 ccctcgccgt cattcagtga ataacgctga atgttgaaaa tgcgcgccac atcattgagg
    19981 cgcgtttcca caacgttaca gctgatgcgc tgtccggcgg atgatgtcat cctggatctc
    20041 cttcgacagt tcgacaaaga aggcgctgta tccggcaacg cgcaccacca gcccggcata
    20101 atcttgtggg cgctgttgcg cttcccacaa cgtgtcggcg ttcaccacgt taaactgaat
    20161 atgctgcaac ttaagctggg taaacgcccg taagaagtcg gccagtttgc gtaatcccgc
    20221 ttcaccttcc agggtcgccg gagtgaattt cacgttcagc aacgtaccgt tagacagcag
    20281 cgtattatcg agcttactga ctgacttcag taccgctgtt ggcccttgtt cgtcctggcc
    20341 cagcataggt gacaagccgc cgtctgccag ctgttctccg gcaaaacgac cgtctggcgt
    20401 cgcgccaacc accgatccca acgggacgtg agcagaaacg gtatacgatc ccggcgtgaa
    20461 gtaaccaccg cgcgggttct ggtatttttc cacttctttg cagtagtggc gcaacagttc
    20521 ggcgctaatg ttatccacct cgtcgatatc gttaccatat ttctcaaagc ggttaattaa
    20581 gcgagcgcgg actttttcgc cttctggcgt tgcaaagttg gcttttaata ccgacagcaa
    20641 ttcgtcaaaa cttaaacgct gttgctcaaa aaccatccct ttcaacgcat ggagagaatc
    20701 gctcaggttg gcgataccga tcccctgtac gccggagaag ttataacgcg cgccgccatc
    20761 ggtaatatcg cgaccttttt ccagacaatc gctgataaaa gacgagagca gcggtacagg
    20821 tgcccagtcg cgatggccga tatcacaaat attgctgccc tcaaccatca gggtgatgta
    20881 gtggctgatc ttggcgcgga tctgctccag taaaccttca taagtcagcg cagcattgcc
    20941 ttcattttca tgcaggcaga tttccatcac tttcagcaga ttaaacatcg cgatgtcatg
    21001 caagccgtag gttctgccgg gaatagataa ttccacacag cccactacgg aatagtcgcg
    21061 cgcatcttcc agcgaaacgc cacggttgag gaacgctggt accaccactt catcgttaaa
    21121 gatttgcgga ataccggtgc cgaggcgaat ggtttcggcg gttttcatca ggaatggcgt
    21181 gtcgatcaag gcgttagtgc gcacgccgag gttcggttgc ggtaattgca cgctttgata
    21241 ggcgtcaagg caaaggaacg aaagcacgtt caccgcgcta cgtccgttct cggttaaccc
    21301 gccgagcagt gcggtatagc cggtcgggaa acctgcgaaa taacgcgcgc tgctggtgga
    21361 gcgcaacagc acgatgtcgt tgcatttcac ccataaagat tcgagcagtt ctttcaggaa
    21421 cgccggatct tcgcccgggg ttaaagatgc ctgatagaac ggcaacatat actggtcgaa
    21481 gcgccctaaa gacagcgaac tggcgttgga ttcgtattgc agaatgatgt tcatgtacca
    21541 gaataactgg cacgcctgcc agaacgtctg cggcttatgt tcggcgttat ggcgagagat
    21601 ctccgcaata gtcagcagtt cttcgcgacg ctgggcatct gtgcagtttg ccgccatcgt
    21661 ttccgccagt acagcgtaac gcaaaatgtg tttctgcgag gcttccagca gtaacagcgc
    21721 tgcctgataa aagtgattct ccggctgttg ctgacaatgt tgctgcatct gtgccaccaa
    21781 ctcccccagc ccgtgattca acaggcgtgg gtaatcaata ataatgtgcc cctggccttt
    21841 atccgtctgg ttaatgctga aaatctgcgt gctggtcgcg gcttttactt catccgtcat
    21901 ctgcccgttg atgaaatctt tcatcgaacg tttttcccag tacgggaaca actcttcacg
    21961 atagatacgt ttatcttctt cgctgatagc aaagcggtcc tgcggacgcg tcgggaattg
    22021 atccagctct ttcagcagcc agtaagggtc catttccggc gacataatcc cggcgcgcgg
    22081 ttttacggtg cggttaccgg caatcagttc ttcatcacga atcgaaattt caacatgttc
    22141 aaggatatac gctgttgctt tcgcccggcg caatatcacc ggttcgcctt cggtttgccg
    22201 atggctggcg gtataaagca gcgcccgctc cagcgagatt tcacgggtat tggcaaacag
    22261 tgcagttttg aggcgagaga tacgattcgt catgaggaac tcctgtggga aagcgaaccc
    22321 gcccttgcga gcgggagaag aacttaagcg gtttgcgcca ggtgagcttc aattttgctc
    22381 ataatggcgt cagcacgttt tactgcatcg ctgatgttga cgcgcacgat ggtcttgcca
    22441 gcaaaacgct cttcaaactt gatgccgata tctttggtca gaatcaccat atcagcgctc
    22501 gccacatctt ccgcagtcag ttcattttcc agaccaattg acccctgggt ttctactttc
    22561 acttcccagc ctttcgcttt cgcggcactt tccagcgcct ctgctgccat ataggtatgg
    22621 gcaacacctg aaggacatgc ggttactgca ataattttcg tcatggctcg ttcctcaatt
    22681 aattaatttc aaaatccaaa tccaggtcgt cttctttttc atcagtcgaa gacccatttt
    22741 tacgcgccag acttttcagc acgttcacac aaacagccgt caccactgct ccgacagcca
    22801 ctgctgcgat ataacccagc ttgccttcaa ccaccggcag cacaatcaga ccaccccagc
    22861 ctgcatagca ctgcgcaccg accagcgccg cagttactgc accacaaact gaaccgacca
    22921 tgatggaagg aatgacacgc agcggatcgg cagcggcgaa aggaatcgcc ccttcagtaa
    22981 cgccaacaca gcccatcacc agtgccgctt tgccggtttc gcgctcttct gcggagaaat
    23041 ttttacgacc aatcagcgtc gccagcccca tccccagcgg cgggatacaa atacccacgg
    23101 cagcgatgac caccacggta taaacaccct gagcaacgca aatcagcatg aaggcatagg
    23161 ctactttgtt aaccggaccg cccatatcga acgccagcat cagacccatg atcaccgcca
    23221 gcataacaat gctgccctgc tgcatcccct gaagccactg agtcaggctg ttggtcaacg
    23281 cccctacagg ctcgcccaag ccccacatca tgatgcctgc ggtaatcagt gtgccgacga
    23341 taggaataat gaagataggc atcaccgagc gcagaacttt atgcaccgga attttcttca
    23401 ggtaatgcac cacgatgccg ccgataatcc cggcgatcag tgcaccaaag aagcccgcac
    23461 caaagctgtt accaacccag gcaccgatag cgcacggagc cagcgcagaa cgctctgcaa
    23521 tggagtaacc aatgtaagcg gcgaggaaag gcaccatcag cgtcaaacct gcaacgccga
    23581 tatcaaacag ttttttcaaa ttcggatcgg ctacggcatc cggcactgcg cctttgccat
    23641 acaacatgac ggaaaccgcc agcaaaatac cgcccgatac cacgaaggga atcatgtgtg
    23701 aaacgcccgt cattaaatgc tgacgggtat ttttcaggat ctgcaccaac tcattcataa
    23761 gctgctcccg ggcttgcgaa agcccatgtc catatttttg atggaggaaa caataggcaa
    23821 tcgcaggcgg agcaaacagt ggataaaaag gccatttact ggacttttgt aatatcagta
    23881 caaaaatgcg atccgcctca taacttgcga taaaccggaa gcatgccgca aaacggcgtc
    23941 ttgtgagacg cgtcgcaaat tgacggttac attacatttg tccagctttt ggcagatttg
    24001 tcacatggca ggccgttgcc gtgcgctctt atcctgtaaa cagaaaaccg taccgggaga
    24061 gaggcaatgg ccctgattgt ggaatttatt tgtgagctac ctaacggcgt acatgcgcgt
    24121 ccggcaagcc acgttgaaac gctgtgtaat actttttcat cacaaattga gtggcataac
    24181 ctgcgcactg accgcaaggg caacgccaaa agcgcccttg cgctgattgg caccgatacg
    24241 ctggcgggcg ataactgcca gttactgatt tccggggccg acgaacagga agcgcaccag
    24301 cgtttaagcc aatggctgcg cgatgaattc ccccactgcg atgcgccgct ggcggaagtt
    24361 aaatctgacg aactggaacc actgccggtt tcactgacca atctgaatcc gcaaattatc
    24421 cgcgcccgca ccgtgtgcag cggtagtgca ggcggcattc tgacgccgat ctcttcttta
    24481 gatctcaatg cgctgagtaa tcttcccgca gccaaagacg ttgacgccga gcaatccgca
    24541 ctggaaaatg gcctgacgct ggtactgaaa aacattgagt ttcgtctgct ggatagcgac
    24601 ggtgctacca gctcgatact ggaagctcac cgatccctgg ctggcgatac atctttacgt
    24661 cagcacttac tgacaggcgt cagcgaggga ttaagctgtg ccgaagcgat tgtcgccagt
    24721 gcgaatcact tttgcgaaga gttcgcccgt tccagcagca gctacctgca agaacgtgcc
    24781 ctggacgtac gcgacgtctg cttccagtta ctccagcaaa tctacggtga gcaacgcttc
    24841 ccggcaccgg gcaaactgac gcagcccgcc atttgtatgg ctgatgaact gactcccagc
    24901 cagttcctcg aactggataa aaatcacctc aaaggtctgt tactgaaaag tggcggcacc
    24961 acctcacata ccgtaattct tgcccgttcg ttcaacattc cgacactggt tggtgtagat
    25021 attgatgccc ttactccgtg gcagcatcaa acgatttata tcgacggcaa cgccggggcg
    25081 attgtggttg agccagggga agccgtggcc cgttattatc agcaagaagc ccgcgtacag
    25141 gacgcccttc gtgagcaaca gcgtgtctgg ctgacccaac aagcccgtac cgctgacggt
    25201 atccgcattg aaattgccgc taacatcgct cactccgtgg aagcgcaggc cgcattcggc
    25261 aatggtgcgg aaggcgttgg tttgttccgc actgaaatgc tctatatgga tcgcaccagt
    25321 gcaccgggcg aaagcgagct gtacaacatt ttttgtcagg cgctggagtc tgccaacgga
    25381 cgcagcatta ttgtgcgcac tatggacatt ggcggcgaca aacccgttga ttatctgaac
    25441 attccggcag aagctaaccc gttcctcggt tatcgcgctg tgcgtattta tgaagaatac
    25501 gcctcgctgt tcaccacaca actacggtcg atcctgcgcg cctccgctca cggcagcctg
    25561 aaaatcatga tcccgatgat ctcctcaatg gaagagatct tatgggtgaa agaaaaactg
    25621 gcggaagcca aacagcaact gcgtaacgaa cacattccgt ttgatgagaa gatccagctt
    25681 ggcattatgc tggaagtacc gtcggtgatg ttcatcatcg atcaatgctg cgaagagatt
    25741 gatttcttta gtattggtag taatgacctg acgcaatatc tgctggcagt ggatcgcgat
    25801 aacgctaagg ttactcgtca ctacaacagc ctgaatccgg cattcttgcg ggcgctcgat
    25861 tacgccgtgc aggcggtgca tcgccagggc aaatggattg gtctgtgcgg tgagctggga
    25921 gcgaaaggtt ccgtgctgcc gttgctggtc ggcttagggc tggatgaact cagcatgagc
    25981 gcaccatcaa ttccggcggc gaaagcgcgg atgacgcaac ttgatagccg tgagtgccgc
    26041 aagttgctca accaggcaat ggcctgccgt acttcgctgg aagtggaaca cctgctggcg
    26101 caattccgca tgacccaaca ggacgcaccg ctggtcaccg ccgagtgcat cacgctggaa
    26161 agtgactggc gcagcaaaga agaagtgctc aaaggcatga ccgataacct gctgctggcg
    26221 ggccgctgcc gctatccgcg taaactggaa gccgacttat gggcgcgcga ggccgttttc
    26281 tctaccggtc tgggctttag ttttgccatt ccacacagca aatcagaaca cattgagcaa
    26341 tccaccatca gcgtggcgcg tctgcaagcg ccggtgcgct ggggcgatga tgaagcgcaa
    26401 ttcatcatta tgttaaccct gaacaaacac gctgcgggcg atcagcacat gcgcattttc
    26461 tctcgcctcg ctcgccgcat catgcacgaa gaattccgta acgcgctggt taacgccgcc
    26521 tctgccgacg ctatcgccag cctgctgcaa catgaactgg aactgtaaaa ggaaacatca
    26581 tggaactgta tctggacacc gctaacgtcg cagaagtcga acgtctggca cgcatattcc
    26641 ccattgccgg ggtgacaact aacccgagca ttatcgctgc cagcaaggag tccatctggg
    26701 aagttctgcc gcgtctgcaa aaagcgattg gtgatgaggg cattctgttt gctcagacta
    26761 tgagtcgtga cgcgcagggt atggtggaag aagcgaagcg cctgcgcgac gccattccgg
    26821 gcattgtggt gaaaatcccg gtgacttccg aaggtctggc cgccattaag atgctgaaaa
    26881 aagagggtat tactacgctt ggcaccgcag tgtacagtgc tgcacaaggg ttactggccg
    26941 cgctggcagg ggcaaaatac gttgctccgt atgttaaccg cgtagatgcc cagggcgggg
    27001 acggcattcg tacggttcag gagctgcaag cgctgttaga aatgcacgca ccagaaagca
    27061 tggtgctggc ggccagcttt aaaacgccgc gtcaggcgct ggactgttta ctggcaggat
    27121 gtgaatccat caccctgccc ttagatgtag cgcaacaaat gctcaacacc cctgcggtag
    27181 agtcagctat agagaagttc gaacacgact ggaatgccgc atttggcact actcatctct
    27241 aaaggagcaa ttatggaccg cattattcaa tcaccgggta aatacatcca gggcgctgat
    27301 gtgattaatc gtctgggcga atacctgaag ccgctggcag aacgctggtt agtggtgggt
    27361 gacaaatttg ttttaggttt tgctcaatcc actgtcgaga aaagctttaa agatgctgga
    27421 ctggtagtag aaattgcgcc gtttggcggt gaatgttcgc aaaatgagat cgatcgtctg
    27481 cgtggcatcg cggagactgc gcagtgtggc gcaattctcg gtatcggtgg cggaaaaacc
    27541 ctcgatactg ccaaagcact ggcacatttc atgggtgttc cggtagcgat cgcaccgact
    27601 atcgcctcta ccgatgcacc gtgcagcgca ttgtctgtta tctacaccga tgagggtgag
    27661 tttgaccgct atctactgtt gccaaataac ccgaatatgg tcattgtcga caccaaaatc
    27721 gtcgctggcg ctcctgcacg tctgttagcg gcgggtatcg gcgatgcgct ggcaacctgg
    27781 tttgaagcac gcgcctgttc ccgtagcggc gctaccacta tggcgggcgg caagtgcacc
    27841 caggcggcgc tggcactggc agaactgtgc tacaacactc tgctggaaga aggcgaaaaa
    27901 gcgatgcttg ccgccgaaca gcatgtagta acccctgcgc tggaacgcgt gattgaagcg
    27961 aacacctatt taagcggtgt tggttttgaa agcggcggac tggcggcggc tcatgcggtg
    28021 cataacggcc tgaccgctat cccggacgcg catcactatt atcacggtga aaaagtggca
    28081 ttcggtacgc tgacgcagct ggtactggaa aacgcgccgg ttgaggaaat cgaaaccgta
    28141 gctgcattta gccatgcggt aggtttgcca ataactctcg ctcaactgga tattaaagaa
    28201 gatgtcccgg cgaaaatgcg aattgtggca gaagcggcat gtgcagaagg tgaaaccatc
    28261 cacaacatgc ctggcggcgc gacgccagat caggtttacg ccactctgct ggtagccgac
    28321 cagtacggtc agcgtttcct gcaagagtgg gaataaccta ctccaaactc ccggcttgtc
    28381 cgggagtttg aacgcaaaat tgcctgatgc gctacgctta tcaggcctac gcaatctctg
    28441 caatatattg aatttgcgtg ctttttaggc cagataaggc gttcacgccg catccggcac
    28501 tctgtgccag taatctgaaa ctcctacgtt gttcaggagt tttttatccg cttctggaca
    28561 ttttctctac agaacaatcg ttagcccgga aggtcgaatc tgtgactctt attttcacgg
    28621 acgaaccaga tgaaagcttc actggcgctg ctcagcctgc taacggcatt taccagccac
    28681 tccttaaaat ctcccgccgt tccaccaacc gtggtacaaa tccaggccaa taccaacctt
    28741 gccatcgcag acggtgccag acagcaaatc ggcaacacgc tattttacga tccggcgtat
    28801 gtgcggctta cctatcctgg cggtgatgtc ccgcaagaac gcggtgtatg ttccgatgtg
    28861 gtgatccgtg cattgcgcag ccagaaggtc gatttgcaga aactggttca cgaagatatg
    28921 gcgaagaatt ttgccgagta cccgcaaaaa tggaagttaa agcgcccgga cagcaacatc
    28981 gatcaccgtc gggtgcctaa tctggaaacc tggtttagcc gccacaacaa aacacgcccc
    29041 atcagtaaaa atgccggtga ctatcaagcg ggcgatattg tctcctggcg attagataac
    29101 gggctggcgc atattggggt ggtgtcagat ggcttcgccc gcgacggtac gccactggtg
    29161 atacataaca ttggcgcagg cgcgcaggaa gaagatgtgc tgtttagctg gcggatggtg
    29221 gggcattatc gttattttgt taaataagcg atcgccccac atgaagaaaa aattcagatg
    29281 ctttttacac tgacagcttc ttttttctta cggctcacca gcgccagtgc cagcacaatc
    29341 agcacaatac cgctaccttc aacggccccc ggattctcac cgagcagcca ccaggaaaag
    29401 agtacgccgc aaacgggaac ggccagcgtg cttaagctgg caatactggc aggcaagttt
    29461 ttcaatacaa acaaccataa gctccacgcc agcgccgtcg ccagaatcgc actgtaggcc
    29521 agcgcccaga acacggtggg ctgccagtca atttcacgtt gcggcaccag taaagcgacc
    29581 acactcatca ccagcgccgc atagagcatc tgccaggatg ttaacgataa taaatccacg
    29641 cgcggatgac gggtgtatag gcgtttagca acaatcgcgc tcgcccccca actaacaccg
    29701 gagaggattg ccagcatggc acttttcatc gaagagaaat cgagttgcca cggctgcaac
    29761 accaaaaata agccgaaagc ggcaatcaga atcgcgaaat attgcccacg tcgcaggcgt
    29821 tcaccgagaa acaacgcggc gaaaatcacc acccagaacg gcatggtata gctcaggatc
    29881 gccaccttcc ccgcacctcc gctgaccaac gcccactgcg ccagaccaac catcccgcag
    29941 gtttgtaaca gggcaatggc taaggtgtat ttaaacggcg tcgggtgcat tccgcgacca
    30001 cgtaataaaa ggacgatgaa taaaacgaga gcgccgaaaa tgcagcgtaa ggcggtaaag
    30061 tcgaaggcac cgatgtaact ggtgacttgc ttcatgaaaa tccagctata actccagata
    30121 agtgtgagca caaccaggcc actgattgcc agtgggttgc tctttcctgc ggcagacatg
    30181 agagatccag tgtgtagttt tcctgtggac actatacgcc ggtaaagaaa cttcagcgac
    30241 tgcctgaaag acagtcactg aagaaggtca gattacagca ggtcgaaacg gtcgaggttc
    30301 atcactttca cccatgccgc tacgaagtct ttaacaaact tctcgtgggc atcgctgctg
    30361 gcgtaaactt ccgctaccgc acgcaggaca gagttagaac caaataccag atccgcacgg
    30421 ctggctgtgt atttcacttc gccggtttcg cggtcacgtc cttcgaacag ctctttcgat
    30481 tcgtcagtcg ctttccactc gtaacgcata tccagcaagt tcacgaagaa gtcattgctc
    30541 aatacgccaa cgcggtcagt gaagacgccg tttttgctgc tatcgaagtt ggcacccagt
    30601 acacgcatcc cgcccaccag cgcagtcatt tccggcgcgg tcagcgtcag ttgctgtgct
    30661 ttgtcgatca gcagtgactc ggtggtggaa acgtccagac gagcgcgata gttacggaaa
    30721 ccgtcagcaa ttggctccag cagctcaaac atctcaatgt cagtctgatc ctgacgcgca
    30781 tcaacgcgac ccggcgcaaa cggtacatga atgctcaaac ctgcggcgct tgcggctttc
    30841 tcaacaccaa ccacaccagc cagcacgatg atatccgcca gcgaggcttt accagactct
    30901 ttctggattt tctccagaac aggcagagca cgaacggctg cggcgttcac atcccagtcg
    30961 cgctgcggca ttaatgccag acgcgcaccg ttggcaccac cgcgtttgtc gccaccacgg
    31021 aaggtagaag cagatgccca ggccaccgat accagctcac taacagacag accagaatcc
    31081 gcaatcgcga atttcagatc gataatgtcc tgctcggtcg ggttgtagat cggctgcggc
    31141 agcggatctt gccagatcag atcttctttc ggcacttccg gcccgatgta gcgagatttc
    31201 ggccccatat ccctgtgcgt cagtttgaac caggcacggg caaaggcttc gttgaacgcc
    31261 tgcggatcgt tgaggaaacg acgagagatc ttctcgaact caggatcaaa acgcagcgtc
    31321 aggtcggtca ccagcattgt cggtttacgt ttcttcgacg gatcgaacgg atccgggata
    31381 atttccggtg cgtctaccgc ttcgaactgg attgctccag ccggactgcg ggtctgtacc
    31441 cactcatatt tgaacaggtt ctcgaagaaa tagttgctcc actgggtcgg cgtctgggtc
    31501 cagacaactt ccagaccaga ggtaatggca tctgcgccaa cgccgctgcc gtaagtgctc
    31561 gcccagccta aaccttgttc ttcaatcggt gcagcttctg gatcaggacc tacatttgat
    31621 gtcggaccgg caccgtgagt tttacccagc gtatgaccac ccgcaatcag cgccacggtt
    31681 tcttcgtcgt tcatgcccat gttgccgaag gtcgcacgga tagctgctgc cgcagaaagc
    31741 ggttcgccgc tgtgatccgg gccttccggg ttaacgtaaa tcagacccat ctcggttgca
    31801 cccagcggtg ctttcgccag cgcttccgga tgacggtgag tcagccaggc tttttcatca
    31861 ccccagttaa catccagatc cggttcccag acgtcttcac gaccggcacc aaaaccgaag
    31921 gtacggaagc cggagttttc cagcgccacg ttacctgcga ggataaacag gtcggcccag
    31981 gagattttct gaccatattt ctgtttgatt ggccacaaca ggcgacgcgc tttatcaagg
    32041 cttacgttat ccggccagga gttcagcggt gcaaaacgtt gctgaccacg acccgcgccg
    32101 ccgcgtccat cgattgaacg gtaagtacct gcgccgtgcc aggccatacg aataaacaga
    32161 ccggcgtagc tgccccagtc ggctggccac cacggctgag attctgtcag cagggctttc
    32221 agatcttttt tcaggccgta gtaatctaat ttgctgaatt ctttgcggta gtcaaagtcc
    32281 tcacccagcg ggttagaacg attagaatgt tggtttaaca ggtcaacacg gagttgattt
    32341 ggccaccagt cgcgagtggt tgtgcccgcc cccgcgctct ggtcgtgacc gccctgatgg
    32401 aacgggcatt tgccagtggc tgtcgtgtta tggatatcgt ctgacgtgct catcaatgtg
    32461 ctcccctcta cagtgttacc gttacgatac acagcgttag agagaagtta taattgaatt
    32521 aatccacgga tgcgatagtt gagatcttac atattgttgg ttaaagagat gtaggtcaaa
    32581 ttgatcgtaa ttaagctttt ttgattatgt ccgaattcgg acaaatgctt tataaaaaag
    32641 gggtttagct tcatatcctt caagtaatga gggagaaaac aaggctgaca accttatttt
    32701 cccgccctca tttcgaggca gcattttgtg ctctgtttaa aatttgtgat cactgtgtga
    32761 ttttcacaaa agccacacta tttataaacc aggtcgaaca cccagcgtat ggcaaatcgc
    32821 gtaactcatt tcagcgcggt taagcgtata gaagtggaaa tctttcactc cttcacggct
    32881 taaaatcttc accatatcca tggcaatatt cgcgcccacc agtttgcggg tttcggcatc
    32941 atcatccagg ccgtcgaaca tttgcgccat ccacgccgga atacgcacgt tggtcatatc
    33001 ggcaaatttc ttcgcctgtt taaagttaga taccggcaaa attcccggaa taatttccac
    33061 atcaatgccc gccgatacac agcggtcacg aaaacgcagg tagctttcga catcgaagaa
    33121 gaactgagta atcgcgcggt tggctccggc atcgactttg cgtttcagat taagcaaatc
    33181 cgcctgagcg ctttttgctt ccgggtgaac ttccggatac gccgccacgg agatatcgaa
    33241 atctgccact tcttttaata gcgtcaccag gtcagaagca tacatttctg gcttaccact
    33301 tcccggcggc agatcgccac gcagcgccac gatatgacga ataccgttat tccagtagtc
    33361 gcgtgcaatg gtgcgcaact cgtcgggcgt cgcatcaatg caagtaagat gcggtgccgc
    33421 ttccagacca gtgcgatctt taatgccttt aataatgctg tgcgtacggt cgcgctcgcc
    33481 ggagttcgcg ccataggtca ccgatacaaa cttcggtttc aggctgctaa ggcgatcgat
    33541 ggagttccac agggtctgct ccatttcact ggtacgcggc gggaaaaact cgaacgaaac
    33601 gttaatctgc ccctggactt ctgccaggcg ctgattcagg gcatcccgct ggctggcgtg
    33661 aaaaaagctc ataccttacc tcatcaatcg cttgtagtta tatgttgtgt tgtgcacatc
    33721 tatccgttta gacgtccaga tgtaaagatg aaggaaaagc cgaagggcgt caaccgaaaa
    33781 atgaccaatg atggtgagga aaaatcaggg aagatgaaaa aacttcagga tggtaagaaa
    33841 aagaaaatcc ggtatgcaaa gcaaaccgga ctgcaaatat aaactaacca tgtacttatt
    33901 gcttcttgcc ggattcgtcg taaacgctta atccggccta atcctatgat aattcaatat
    33961 atttcattag aaaccataaa cccgaaaaca tgagtaccgg gcattattaa atttctgaaa
    34021 ttacaacaac tgtgccagcc ggttgatatc cgactgaatc gccccggcgg tgacgtcgcg
    34081 ccccgcgcca ggcccacgga tcaccagcgg gttgtcgcga taccagcggc tttcgatggc
    34141 aaagacgtta tcgcacggca gcagtgatgc cagcggatgg tcttcacgca ccgcctccac
    34201 gcccacacgc gctttgccgt tggcatcgaa acgcgccacg tagcgcagca ccagccccat
    34261 ttcgcgggcc gcttccagcc gttgcaccat ctgctcgtta agttcatcgc cattttcaaa
    34321 gaaatggtcg atgctgccgc cttcgcaatg agcaggcacc agcgattcca cacgtacctg
    34381 gtccggttca atgttgtaac ccgcttcacg cgccagaatt accagcttgc gcattacgtc
    34441 tttgccggag aggtcgtcac gcggatcagg ctcggttaag ccctgctgcc acgcctgatc
    34501 caccagctcg gtaaacggca cgctaccgtc gaattgcagg aacagccagg agagcgtgcc
    34561 ggagaagatc ccgctgatcg acaaaatagt atcgccgctg tcgatcagat cgcgcacggt
    34621 gtggttgatc ggcaagcccg caccgacggt ggcattgtac agccagtgac gcccggtttt
    34681 ttcgaaggcg tcgtggatct ggcgatattt attgctgtcg ctggctcccg ccagtttgtt
    34741 ggcgctgata acgtggaaac cgtggctggc gaaatcaaga tactgatcag caagctgctg
    34801 gctggcggta acgtccagca ccactaaatc atcatacgga tgggcgcgca tccacaggaa
    34861 caacgactct tcatcctgct caaccgcttc atcgttgaag aaggctaacg cgcggctggc
    34921 gtccagcccg tcatagctca acaggctacg gcggctgtcc accacacctg ccagcacaaa
    34981 ctcaaagccg gtacgtgccg aaagcgtgct ctgctcacgg gcgaacagtt ccagccaacg
    35041 ggaaccgata ttgcccttac cgaacaatac caggccgatg cgtttttctg cgcggaagac
    35101 ggactgatgc agcccctgaa tcaggctttc ggtcgggccg gtgcgcagta ctgccaccag
    35161 gctgatgccg tcatcggact gccaggtaaa ttcgaccggc tggcctttca gttgctgcca
    35221 gaagcggtgg caatgcagcg ggttacgggt gacgcctgca ccgaccatcg ccaccagcgc
    35281 cagcccctga cgcaggcgca gttcgccagg taatcccgct tcgtcgagga ttttcagcgc
    35341 actgtcggcc acttctgagg tgtagcaaaa ttgcagcaac tggcgatcgt tatgtacgcc
    35401 aaccgccagc gggcgtacct gcgcgcgttt caggatttgg tcgatctctt tatgcgccag
    35461 tttgaaatcc tgactggcgg gcacctgaaa ctcaatcaaa cagacatcat cgtggctggt
    35521 gacaatacgc gcaccagtac cggaggccag cacgcgttca atgcgcgtgg aaccttgatc
    35581 cggcgtgtag ctacagcgca gttgcaggtc gatttcgctg ccagaaaccg gctgtaaagt
    35641 acgggcgtga agaacgggag ccgccaggcg cgccagttcg ctggcctcat ccagacgcag
    35701 caacggcagc aggcaggcat ctttcacttt acgcgggtcg gcactgtata ccccggcgac
    35761 gtcgctccag atggttacgc gagaaacacc cgccagcgca ccgatttgtg tcgcggaata
    35821 gtcggaaccg ttacgcccca gcagcaccgt ttcaccggcg ttgttgcggc tgataaatcc
    35881 ggtcaccacc agacgtttgc ccggatgttg caccagcagc tgttgcagca acgggtaaga
    35941 aagcccttca tcaacctgcg gttgtgcggc gcgttcagcg cgtaaaaact cgcgggcatc
    36001 aagccaggcc gctggcagcc cttgttgatt aagtaccgca gacatcaggc gtgccgacca
    36061 tacttccccg tggcccacca cttccgcata cactgcgtcg ttaataccgc tgtcgagcag
    36121 cgccgccagg cgctcaaggt cgctgacaaa agcgctaatg aggctatcag cttcttcagc
    36181 gggtagcaga ccgctaatca gatcgcactg atagcgacgc agcgtttgtt gaacctgatg
    36241 cgcagagaga cgatcggtct ggcttagttt caaccagtta atcaactggt tagtggtgct
    36301 accggcggcg gaaaccacca tcatatcgtc aggctgagag tactccgcca taatgcccgc
    36361 aacacgcaaa taacacttca catcagccag actactgcca ccaaatttat gcagttgacg
    36421 acctttcgcc cctgcctgcg caatcacact catttttacc ccttgtttgc agcccggaag
    36481 ccattttcca ggtcggcaat taaatcttcg ccatcttcaa taccggtgga gatacgcagc
    36541 agcgtctcgg agatcccggc ggcagcacgc gcttctggtg ccatgcctgc atgtgtcatg
    36601 gttgcggcgt gagagattaa actttccact ccccctaatg attccgccag cgtaaacaac
    36661 gacagcccgc ccaggaaacg acgcagcgtc tgctcatcgc catccagttc aaaactcaac
    36721 attgcgccaa agcctttttg ctggcgcgcg gcaatttcat gcccctgatt ttccggcagc
    36781 gacgggtgat acagtttttt caccaacggc tgggtttgca gatatttcac aatcgcctgc
    36841 gcgttgcgct gggccagctc catacgcggc accagcgttc gcaacccgcg tagcagcaga
    36901 tagctgtcaa acgcgccgcc cgtcacgcca atattgtttg tccaccaggc aagttcagtg
    36961 acaacgtccg ggtctttagc aatcaccacg ccagccacca cgtcagagtg accgttcaga
    37021 tatttcgtac atgaatgcaa caccagatcg gcacccaatg ccagcggatt ttgtaatgcc
    37081 gggcttaaga aggtgttatc caccacgctc accgccccga cttcccttgc cagatggcag
    37141 attttcgcaa tatccacgac gcgtaacaat ggattacttg ggctttctac cagtaccagt
    37201 ttaggttttt ctgccagcgc tgcccgtaat gcctgttcat cgccttgatc aacaaacaac
    37261 acgcgatagc aaccgcgttt cgccagactg tcgaacaggc gatagctacc gccgtagcag
    37321 tcgtgcggcg caaccagcag atcgccaggt ttcaaaaaga cggtcgttac caagtgaatc
    37381 gcggacatgc cggtattagt aagtactgcg ccagcaccgc cttccagttc tgccagcgca
    37441 cgctgaacca catcgcgcgt tgggttgccg cgacgtgagt aatcatgcgc gcgcggttca
    37501 ttaaatccgg taaagttata ggtgctggaa agatggatgg gagggacaac gcaaccatac
    37561 tgttcgtcgt catttaaccc gctacgcact gcgatggtgg cctgtttacg cgtcatgtga
    37621 tgaagtttcc tgggctttgt cggtgaaatg tcaggcacca gagtaaacat tgtgttaatg
    37681 gacgtcaata catctggaca tctaaacttc tttacgtata gattgagcaa atcccaaata
    37741 gccgttaaaa ttatatgcat tatcacgccg acaggtgcat tacacgatgt cacggtaacg
    37801 cctgtacggt aaactatgcg ggtttacggt cagtacccac ataaattgtg tggtctggtc
    37861 tcaatttatt gacgaagagg attaagtatc tcatggctga atggagcggc gaatatatca
    37921 gcccatacgc tgagcacggc aagaagagtg aacaagtcaa aaagattacg gtttccattc
    37981 ctcttaaggt gttaaaaatc ctcaccgatg aacgcacgcg tcgtcaggtg aacaacctgc
    38041 gtcacgctac caacagcgag ctgctgtgcg aagcgtttct gcatgccttt accgggcaac
    38101 ctttgccgga tgatgccgat ctgcgtaaag agcgcagcga cgaaatcccg gaagcggcaa
    38161 aagagatcat gcgtgagatg gggattaacc cggagacgtg ggaatactaa agcaaaaaag
    38221 agcggcgcgg agtggaatcg cctgatgcgc tacgcttatc aggcctacgt catattgcaa
    38281 tttattgaat ttgcacgaac ttgtaggccg gataaggcgt tcacgccgca tccggcataa
    38341 acaacgagca cgttgtctgc gacccaccgc tttttataca tggacgttta actatgaaaa
    38401 acaggctgct gatcctcagc ctgctggttt ctgtacctgc ctttgcctgg cagccacaaa
    38461 ccggcgacat catctttcag atctctcgct catcgcaaag taaagcgatc caactggcga
    38521 cccattccga ttatagccac accggtatgc tggtgatacg caacaaaaag ccctacgttt
    38581 ttgaagcggt cggcccggtg aaatacaccc cgctcaagca gtggatcgcc catggtgaaa
    38641 agggcaaata cgttgttcgc cgcgttgaag gcggactgag tgttgaacaa cagcaaaaac
    38701 tggcgcaaac ggtaaaacgt tatctcggta aaccgtatga tttcagcttc tcatggagcg
    38761 acgatcgcca gtactgttcg gaagtggtgt ggaaggttta ccagaacgcg ctgggaatgc
    38821 gcgtgggcga gcagcagaag ttgaaagagt tcgatctcag caacccgctg gtccaggcta
    38881 agctcaaaga acgctacggc aaaaatattc cgcttgagga aacggtagtt tcaccacaag
    38941 ccgttttcga cgcgccacaa ctcaccacgg ttgccaaaga gtggccgctg ttttcgtggt
    39001 aatggatgat gaaaaaaata attcccgccg tagtctgttt gttgctttgt agttgcacga
    39061 cctttccgca aactgtcgct ccggtaaacg gtgatgcctc caattagttg aattaaggat
    39121 ataatactcg cttttaaggt tgtttcatgg tcacagttaa ccttcattgc ccccgttgcc
    39181 agtccgttca ggtttaccgt cacgggcaga acccaaaaag acatgatcga ttccgcggcc
    39241 gtgactgtca ccgggtattt cagctcagtt actgttacga gccccgtaaa ccgggagtta
    39301 aagaccaaat cgctgaaatg actttcaacg gtgccggggt tcgcgatact gcccgtacgc
    39361 tgaaaatcgg cattaataca gtcatccgaa ctttaaaaaa ctctcgccac gaagaataac
    39421 gtcatccccg gtcgctcatg ctgatgtggc actcatctgc gaacttagtc agcaatggag
    39481 ctttgtcggc agtaaagcgc gacagcactg gctctggtac gtgtacaaca cgaaaactgg
    39541 tggtgttctg gcctacactt ttggcccccg gactgacgaa acctgtcgtg aattgctggc
    39601 gctgcttacc cttttaccat cggcatgttg accactgacg aatggggtag ttacaccaga
    39661 gagttaccga aggaaaagca tctgactggt aagattttta ctcagcgcat tgagcgcaac
    39721 aatctgaccc tgagaacccg aatcaaacgt ctggcccgca gaaccatctg tttctcgcgc
    39781 tctgtagaac ttcatgaaaa agtcatcggt gcttttatcg aaaatatatg ttctactaat
    39841 tggagtcatc acccttctga tgagctttcc ttggatgatt accagactga atacacagcc
    39901 aaagattttt acctcattct gctctttttt ctcaaatgac atatattata ttttacatca
    39961 tattcatagg catatgacat tcccccgccc ccattatgtc attgtgggtt gatgttgggc
    40021 tttcataaaa aaaacatctt tttttgaact agcattaatt atatttactt tatatattaa
    40081 aatattttct ccatctggaa atattttagc cacacaatat aaagcagctg aatccgaatt
    40141 acttaaaaat atattatcat tacccataac tccagccatt aaattactgg agactaaagc
    40201 aaggaaataa caacattgta ttgccttcat tttaattact cccttgtctg ataattgtaa
    40261 ctccattccc tttagctttg ggattatatt ttagataggt gttatttggt gttcccaagt
    40321 aactacttgt attctttttt tcccattcca ttcatataag aattcgttaa atctttgctt
    40381 gaaaaattta gactatcata aacatcattt tcttttgtta ctttatttcc tttatcgtca
    40441 gagtaaaaac cgtgagtgtg ataatcgcct actctctctg aaccttcagg gcattttata
    40501 tttcgcaagt ctacactatc attatcattt gaacttatcg gattcatggg gaaatattca
    40561 ccttgcttct tacatattag tccaccatat tctttattct cgttaattga ttctccatta
    40621 cacat
//
LOCUS       DAJTSL010000013        40430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTSL010000013 DAJTSL010000000
VERSION     DAJTSL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.013"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(117..2438)
                     /locus_tag="OO433_000616"
     CDS             complement(117..2438)
                     /locus_tag="OO433_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6049205.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFITRYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2535..3011)
                     /gene="greB"
                     /locus_tag="OO433_000617"
     CDS             complement(2535..3011)
                     /gene="greB"
                     /locus_tag="OO433_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6049206.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            3239..3958
                     /gene="ompR"
                     /locus_tag="OO433_000618"
     CDS             3239..3958
                     /gene="ompR"
                     /locus_tag="OO433_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6049207.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            3955..5307
                     /gene="envZ"
                     /locus_tag="OO433_000619"
     CDS             3955..5307
                     /gene="envZ"
                     /locus_tag="OO433_000619"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6049208.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5383..7005)
                     /gene="pckA"
                     /locus_tag="OO433_000620"
     CDS             complement(5383..7005)
                     /gene="pckA"
                     /locus_tag="OO433_000620"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6049209.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7384..9108
                     /locus_tag="OO433_000621"
     CDS             7384..9108
                     /locus_tag="OO433_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6049210.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNCSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDVTFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9243..10121)
                     /gene="hslO"
                     /locus_tag="OO433_000622"
     CDS             complement(9243..10121)
                     /gene="hslO"
                     /locus_tag="OO433_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6049211.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSREHCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10146..10547)
                     /gene="hslR"
                     /locus_tag="OO433_000623"
     CDS             complement(10146..10547)
                     /gene="hslR"
                     /locus_tag="OO433_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6049212.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10558..11226)
                     /gene="yrfG"
                     /locus_tag="OO433_000624"
     CDS             complement(10558..11226)
                     /gene="yrfG"
                     /locus_tag="OO433_000624"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6049213.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11291..13426)
                     /gene="igaA"
                     /locus_tag="OO433_000625"
     CDS             complement(11291..13426)
                     /gene="igaA"
                     /locus_tag="OO433_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6049214.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRSQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLDQQTDIDIYLDCHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13746..14306
                     /gene="nudE"
                     /locus_tag="OO433_000626"
     CDS             13746..14306
                     /gene="nudE"
                     /locus_tag="OO433_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6049215.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14471..17023)
                     /gene="mrcA"
                     /locus_tag="OO433_000627"
     CDS             complement(14471..17023)
                     /gene="mrcA"
                     /locus_tag="OO433_000627"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6049216.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17143..17922
                     /gene="hofM"
                     /locus_tag="OO433_000628"
     CDS             17143..17922
                     /gene="hofM"
                     /locus_tag="OO433_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6049217.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALFVDPDS
                     VAICGEGGFDPWEAVSVRQPLLPPPGGDFAIALGLALGKAY"
     gene            17922..18461
                     /gene="hofN"
                     /locus_tag="OO433_000629"
     CDS             17922..18461
                     /gene="hofN"
                     /locus_tag="OO433_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6049218.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRKQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18445..18885
                     /gene="hofO"
                     /locus_tag="OO433_000630"
     CDS             18445..18885
                     /gene="hofO"
                     /locus_tag="OO433_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6049219.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18875..19279
                     /gene="hofP"
                     /locus_tag="OO433_000631"
     CDS             18875..19279
                     /gene="hofP"
                     /locus_tag="OO433_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR6049220.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            19191..20429
                     /gene="hofQ"
                     /locus_tag="OO433_000632"
     CDS             19191..20429
                     /gene="hofQ"
                     /locus_tag="OO433_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6049221.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGIEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEYERRELVVFITPRLVSSE"
     gene            20830..21351
                     /gene="aroK"
                     /locus_tag="OO433_000633"
     CDS             20830..21351
                     /gene="aroK"
                     /locus_tag="OO433_000633"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6049222.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            21408..22496
                     /gene="aroB"
                     /locus_tag="OO433_000634"
     CDS             21408..22496
                     /gene="aroB"
                     /locus_tag="OO433_000634"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6049223.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            22588..23874
                     /gene="damX"
                     /locus_tag="OO433_000635"
     CDS             22588..23874
                     /gene="damX"
                     /locus_tag="OO433_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6049224.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGDKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            23981..24817
                     /gene="dam"
                     /locus_tag="OO433_000636"
     CDS             23981..24817
                     /gene="dam"
                     /locus_tag="OO433_000636"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6049225.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            24835..25512
                     /gene="rpe"
                     /locus_tag="OO433_000637"
     CDS             24835..25512
                     /gene="rpe"
                     /locus_tag="OO433_000637"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6049226.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            25505..26263
                     /gene="gph"
                     /locus_tag="OO433_000638"
     CDS             25505..26263
                     /gene="gph"
                     /locus_tag="OO433_000638"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6049227.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            26256..27260
                     /gene="trpS"
                     /locus_tag="OO433_000639"
     CDS             26256..27260
                     /gene="trpS"
                     /locus_tag="OO433_000639"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6049228.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            27529..28434
                     /locus_tag="OO433_000640"
     CDS             27529..28434
                     /locus_tag="OO433_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049229.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            28451..28813
                     /locus_tag="OO433_000641"
     CDS             28451..28813
                     /locus_tag="OO433_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6049230.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            28897..30060
                     /locus_tag="OO433_000642"
     CDS             28897..30060
                     /locus_tag="OO433_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6049231.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            30060..31286
                     /locus_tag="OO433_000643"
     CDS             30060..31286
                     /locus_tag="OO433_000643"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6049232.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVDAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPLLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            31283..32161
                     /locus_tag="OO433_000644"
     CDS             31283..32161
                     /locus_tag="OO433_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6049233.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            32172..32525
                     /locus_tag="OO433_000645"
     CDS             32172..32525
                     /locus_tag="OO433_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6049234.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            32537..33841
                     /locus_tag="OO433_000646"
     CDS             32537..33841
                     /locus_tag="OO433_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6049235.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            33853..34938
                     /locus_tag="OO433_000647"
     CDS             33853..34938
                     /locus_tag="OO433_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6049236.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRCLVFAPVMHAVQAGVSERLLALLNGGEVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35090..35821)
                     /gene="phoB"
                     /locus_tag="OO433_000648"
     CDS             complement(35090..35821)
                     /gene="phoB"
                     /locus_tag="OO433_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6049237.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCKKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(35921..36706)
                     /gene="frlD"
                     /locus_tag="OO433_000649"
     CDS             complement(35921..36706)
                     /gene="frlD"
                     /locus_tag="OO433_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6049238.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(36703..37533)
                     /gene="frlC"
                     /locus_tag="OO433_000650"
     CDS             complement(36703..37533)
                     /gene="frlC"
                     /locus_tag="OO433_000650"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6049239.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELYEYAENIGMDLILEPLTPYESNIVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(37583..38605)
                     /gene="frlB"
                     /locus_tag="OO433_000651"
     CDS             complement(37583..38605)
                     /gene="frlB"
                     /locus_tag="OO433_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6049240.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQAAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(38626..40014)
                     /gene="frlA"
                     /locus_tag="OO433_000652"
     CDS             complement(38626..40014)
                     /gene="frlA"
                     /locus_tag="OO433_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR6049241.1"
                     /translation="MTANSPLQRVGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLTFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISDLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGLFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            complement(40259..40426)
                     /locus_tag="OO433_000653"
     CDS             complement(40259..40426)
                     /locus_tag="OO433_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6049242.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         9346 a        10255 c        11119 g         9710 t
ORIGIN      
        1 gttgtggaat aaaaaggccg gagatattct ccggcacttt tatgtcaggc aaggcgttcg
       61 ttgccagatg cggcgtgaac gccttatcgc gcaggtttag gggccagtcc gggcgtttaa
      121 cgttttttgc ccattgccgc cgccagcgca tccatcatcg cgctattacc ggcaggctgc
      181 gcttcacgac cgcgtggttt ggctgccggg cggttgtttt gcgggcgttc attaccgccg
      241 ccgcgacggg cgttggtttc gccaggctgc tcgtccagac gcatagtcag ggcgatacgt
      301 ttacgctgaa gatccacttc cagcactttc accttcacaa tgtcgcccgc tttcaccacg
      361 gtatgcggat cttccacaaa cttgttcgac aatgaagaga tgtgaaccag gccgtcctga
      421 tgcacgccga tatcgacaaa cgcgccaaag ttggtgacgt tggtcaccgc accttcgagg
      481 atcatacccg gttgcaggtc gttcattgtc tcgacgccat cggcaaactg agcggtttta
      541 aattccggac gcggatcgcg acccggtttt tccagctctt tgatgatgtc agttactgtc
      601 ggcacaccga atttttcatc agtaaagtca gacgctttca ggttacgcag ttcgctgctg
      661 ttacccatca gatctttcag tgcctgctgt gttgctgcca gaatgcgttc caccaccgga
      721 taggcttccg ggtggacggt agaagcatcc agcgggttat caccgtggtt aatgcgcaag
      781 aagcccgcgc actgctcgaa ggctttcggc cccagacggc tgactttcag cagttgctga
      841 cggttctgga actggccgtt ctcatcgcgc caggccacga tgttttgcgc catcatgcgc
      901 gtcaggcccg ccacgcgggt taacagcgga acagaagcag tgttgagatc gacgccaacg
      961 gcgtttacgc agtcttctac tactgcgtcc agtttgcggg ccagttgcgt ctggctgacg
     1021 tcatgctgat actgacctac gccgatagat ttcggatcga ttttcaccag ctccgccagc
     1081 ggatcctgca aacggcgggc gatagacacc gcgccacgca gcgaaacgtc gagatccggg
     1141 aactcctgcg ctgccagctc ggaagctgag taaaccgacg cgccagcttc gctgacgatc
     1201 actttctgtg cggtcacttt cgggaactgc ttctgcacgt cgagatagaa acgttcagtt
     1261 tcgcgggaag ctgtaccgtt gccgatcgcc accagttcaa cgttatgttt ttcacacaag
     1321 gcagcaacgg tcatcgctgc ttttgcggct tgtccggtgt gcgggtaaat ggtatcggtc
     1381 gctaccagtt tgccagtggc atcgaccacc gccactttta ccccagtacg cagacccgga
     1441 tcgaggccca tcgttgcacg cagtccggca ggggccgcca tcagcagatc gtgcaggtta
     1501 cgggcaaaga cgttgattgc ttcatcttcc gcgcgttcgc gcacggtacc catcagttcg
     1561 gtttccagat gcatcagcac cttgatgcgc caggtccagc tcactacgcc tttgcgccag
     1621 ctatccgccg gggcattgtt caggcgcagg ccaaggtgat ccatgatgat ttgctcgcaa
     1681 tagctctctt tgggcggctc atcgaactgc ggatcggcat tcagcgaaag ctggagtacg
     1741 ccttcgttac gcccacggaa catcgccaga gcgcggtgag aaggcaccgt ggacaacggt
     1801 tcgtgatgat cgaaatagtc gcggaatttc gccccttcct cttctttacc gctcaccacc
     1861 gtagaaacca aatgcgcgtt cttccacaga taatcacgca ctttcgccag cagcgcggca
     1921 tcttcggcaa accgttccat caggatatag cgcgcgccat ccagcgcggc tttggtatct
     1981 gccacgcctt tatcggcatc aacatattgt gcagcggcga cttctggcgt gtgtgacgga
     2041 tcgctccaca gcaggtcagc caacggctca agccctgctt caatggcgat ttgcccgcgg
     2101 gtgcggcgtt taggtttgta gggcaggtag aggtcttcga gttcggtttt gcttagggtg
     2161 gcgttgatgg ccttcgccag atcatcggtg agtttgcctt gctcggaaat ggacttgagg
     2221 atcgcctgac gtctctcttc cagctcgcgc agatagctca gacgcgtttc cagattacgc
     2281 agctgcgtgt catccagacc gccggtgatt tccttacgat aacgtgtgat aaacggcacg
     2341 gtattccctt cgtcaagcag gcgaacggca gcgtcaacct gttccgggcg cgcctgaatt
     2401 tcacccgcaa taatgcggca gaacgaatca ttcatcatgg tttgagttca tcttttcgga
     2461 tcaaaaatca ggggatagtt atacggactg gctggcaaaa atgccagcca tcggcaggag
     2521 gttaagactc ttccttacgg tttcacgtac tcgatagcat taacatacca gctcgcttcc
     2581 ccggcagggg tattcaccac cgccagatcg ccgacttctt ttttcagcaa tgcgcgggcc
     2641 atcggggaat cgatagagat gtaatcttta cggccaaaaa tttcatcgta gccgacaata
     2701 cggaaacggt gagtcacgcc atcgtcgttt tcaatctcca cccacgcgcc aaaaaagact
     2761 ttgccttcct gctgagggga gtaatcgacg attttgagat tttccatgca tttagtgaga
     2821 tagcgcacgc gacggtcgat ttcacgcaga cgctttttat tatactgata gtcagcattt
     2881 tcgctgcggt cgcccagact tgcggcccag gtcacctttt ttgtgacctc cgggcgttct
     2941 tcacgccaga gataattaag ctcttgtttg agtttttcat acccttcccg ggtaaccagg
     3001 ggcgttttca tctcgttgat tccctttgtc tgtttgataa tgcgcacatt gggtataacg
     3061 tgatcatatc aacagaatca ataatgtttc gccgaataaa ttgtatactt aagctgctgt
     3121 ttaatatgct ttgtaacaat ttaggctgaa attcatacca gatttagctg gtgacgaacg
     3181 tgagcttttt taagaataca cgcttacaaa ttgttgcgaa cctttgggag tacaaacaat
     3241 gcaagagaac tacaagattc tggtggtcga tgacgacatg cgcctgcgtg cgctgctgga
     3301 acgttatctc accgaacaag gcttccaggt tcgaagcgtc gctaatgcag aacagatgga
     3361 tcgcctgctg actcgtgaat ctttccatct tatggtactg gatttaatgt tacctggtga
     3421 agatggcttg tcgatttgcc gacgtcttcg tagtcagagc aacccgatgc cgatcattat
     3481 ggtgacggcg aaaggggaag aagtggaccg tatcgtaggc ctggagattg gcgctgacga
     3541 ctacattcca aaaccgttta acccgcgtga actgctggcc cgtattcgtg cggtgctgcg
     3601 tcgtcaggcg aacgaactgc caggcgcacc gtcacaggaa gaggcggtaa ttgctttcgg
     3661 taagttcaaa cttaacctcg gtacgcgcga aatgttccgc gaagatgagc cgatgccgct
     3721 caccagcggt gagtttgcgg tactgaaggc actggtcagc catccgcgtg agccgctctc
     3781 ccgcgataag ctgatgaacc ttgcccgtgg tcgtgaatat tccgcaatgg aacgctccat
     3841 cgacgtgcag atttcgcgtc tgcgccgcat ggtagaagaa gatccagcgc atccgcgtta
     3901 cattcagacc gtttggggcc tgggctacgt ctttgtaccg gacggctcta aagcatgagg
     3961 cgattgcgct tctcgccacg aagttcattt gcccgtacgt tattgctcat cgtcaccttg
     4021 ctgttcgcca gcctggtgac gacttatctg gtggtgctga acttcgcgat tttgccgagc
     4081 ctccagcagt ttaataaagt cctcgcgtac gaagtgcgta tgttgatgac cgacaaactg
     4141 caactggagg acggcacgca gttggttgtg cctcccgctt tccgtcggga gatctaccgt
     4201 gagctgggga tctctctcta ctccaacgag gctgccgaag aggcaggtct gcgttgggcg
     4261 caacactatg aattcttaag ccatcagatg gcacagcaac tgggcggccc gacggaagtg
     4321 cgcgttgagg tcaacaaaag ttcgcctgtc gtctggctga aaacctggct gtcgcccaat
     4381 atctgggtac gcgtgccgct gaccgaaatt catcagggcg atttctctcc gctgttccgc
     4441 tatacgctgg cgattatgct attggcgata ggcggggcgt ggctgtttat tcgtatccag
     4501 aaccgaccgt tggtcgatct cgaacatgct gctttgcagg tcggtaaagg gattattcca
     4561 ccgccgctgc gtgaatatgg cgcgtccgag gtacgttcgg ttacccgcgc ctttaaccat
     4621 atggcggctg gtgttaagca actggcggat gaccgcacgc tgctgatggc gggggtaagt
     4681 catgacttac gcacgccgct gacgcgtatt cgcctggcga ccgagatgat gagcgaacag
     4741 gatggctacc tggccgaatc gatcaataaa gatatcgaag agtgcaacgc catcattgag
     4801 cagtttatcg actacctgcg caccgggcag gagatgccga tggaaatggc ggatctcaac
     4861 gcagtactcg gtgaggttat tgctgccgaa agtggctatg agcgggaaat tgaaaccgcg
     4921 ctttaccccg gcagcattga agtgaaaatg cacccgctgt cgatcaaacg cgcggtggcg
     4981 aatatggtgg tcaacgccgc ccgttacggc aatggctgga tcaaagtcag cagcgggacg
     5041 gagccgaatc gcgcctggtt ccaggtggaa gatgacggtc cgggaattgc gccggaacaa
     5101 cgtaagcacc tgttccagcc gtttgtccgc ggcgacagtg cacgcaccat tagcggcacg
     5161 ggattagggc tggcaattgt gcagcgtatc gtggataacc ataacgggat gctggagctt
     5221 ggcaccagcg agcggggcgg gctttccatt cgcgcctggc tgccagtgcc ggtaacgcgg
     5281 gcgcagggca cgacaaaaga agggtaaata aacgggaggc gaaggtgcct cccgttttgc
     5341 tttctataag atactggata gatattctcc agcttcaaat cattacagtt tcggaccagc
     5401 cgctaccagc gcggcacccg caggggtgtc ggtgtattta tcgaagttgt cgataaacag
     5461 tttcgccagg gtttcggctt tttcctgcca ctgttccgga gaagcgtagg tgttacgcgg
     5521 atcgagaatc ttcgtgtcta cgcccggcag ttcggttggg atcgccaggt taaacatcgg
     5581 cagagtgaag gtttctgcat tatccagcga accgttgagg atggcgtcga taatggcgcg
     5641 ggtatcttta atcgagatac gtttgcctgt gccgttccag ccagtgttaa ccagataagc
     5701 ctgcgcgccc gccgcctgca tacgtttcac cagcacttct gcgtactgag tcgggtgcag
     5761 cgacaggaat gccgcgccga agcaagcgga gaaggttggc gtcggttcgg tgatgccacg
     5821 ctcagtaccg gccagtttgg cggtgaagcc agagaggaag tgatactggg tttgatcggc
     5881 agtcaggcga gaaaccggcg gcaacacgcc gaaagcatca gcagtcagga agataacctt
     5941 agtcgcgtgg cccgctttgg aaaccggctt aacgatgtta tcgatgtgat agattggata
     6001 agaaacgcgg gtgttctcgg tttttgaacc atcatcaaag tcgatagtgc catcttcacg
     6061 cacggtgacg ttttccagca gcgcatcacg acggatagcg ttgtagattt caggttccgc
     6121 ttctttcgac agcttgatag tttttgcgta gcagccgcct tcgaaattaa acacgccgtc
     6181 atcgtcccag ccgtgttcgt catcgccaat caggcggcgt ttcgggtcgg tggaaagggt
     6241 ggttttaccg gtgccggaaa ggccgaagaa caccgcaaca tcgcctttct cgccaacgtt
     6301 ggcggagcag tgcatagaag cgataccttt cagcggcagc aggtagttca tcatcgagaa
     6361 catccctttc ttcatttcgc cgccgtacca ggtgccgcca atcagctgca tgcgctcggt
     6421 caggttaaac gccacgaagt tttcggagtt cagaccctgt tctttccact gcgggttagt
     6481 gcacttcgcg ccgttcataa cgataaagtc tggtttgaaa cctgccagct cttcatcgct
     6541 cgggcgaata aacatgtttt tgacaaaatg cgcctgccag gccacttcgg tgatgaaacg
     6601 gacggaaaga cgagtatccg ggttcgcacc acagaaagcg tcgacaacga acagacgttt
     6661 gccggaaagc tgcctggtca ccaggccttt cagatgctgc caggtttccg gagagagagg
     6721 tttgttgtcg ttcttacctt tgcctttgtc tgcccaccag aaagtatcgc gagtggtatc
     6781 gtcacggacg atatacttat cttttggtga acgaccggtg aagatcccgg tatcgacggc
     6841 aacggcaccc agattagtta acaccccgcg ctcataacct gtcaggctcg gatcgagctc
     6901 ttcctgatac agcaggtcgt agcttgggtt gtaaacgata tcatgtacgt cactgatacc
     6961 ataagcctcg agttcttgcg gggtcaaacc attgttaacg cgcatttcac tgctccttag
     7021 ccaatatgta ttgcctgaat agtaaagtct ttttgggggt gttaaccgcg acaaggctca
     7081 tagatttacg tatctggaga aattcatatt tgacaaaaaa ccccgtgatt cctgttacga
     7141 aacggttgct attatcgcag aaatagcatt ttaggtgtgg aaaatgttct caaaaggtta
     7201 aatcttggtt tatatggcgg gaatattgtg agtggaatcg cattccttaa tgaaattgat
     7261 gcaaattcaa tcggtaaagt atcgattcac cacgctaact ttttttgatt attttctgaa
     7321 aaaagcaaaa tactactgcc cggatgcgat aattaaccca ccttttgatt ctggataacc
     7381 aacatggata acgttgaact ttcaccggca acacgctggg ggatgattgc taccggatta
     7441 cttcaggggc tggtttgcta cctgttgatc gcctggcttg ccggaaaaaa tcacagttgg
     7501 attgtttatg gcgtgcctgc gaccgttgcg ttttcatccg ttttactctt ctccgtgatc
     7561 tcttttaaac agaagcgcct ttggggatgg ctggcgctgg tgtttattgc cacgacaggg
     7621 atgagcggtt ggctgaagtg gcaaactgat ggcatgaacc cctggagagc tgaaaaggcg
     7681 atttgggatt ttggctgcta tctgctgttg atggcaatgt tgttgctacc gtggatacaa
     7741 caaagccttc gtatccgcaa ttgcagtagc cgctatagct acttttacca gtcagtatgg
     7801 cataacgtac ttatattact ggtgattttt ctcgccaatg gcctgacgtg gctggtgctt
     7861 ttactgtggg gtgagttgtt taaacttgtt ggcatcaagt ttttcaatac gctctttttt
     7921 gcaaccgact ggtttatgta tcttacatta ggtctggtta ccgcactggc ggtgattctc
     7981 gcgcgcacac agtcacgttt aatcgactct attcaaaagt tgttcacgtt aatcgccacg
     8041 gggttgctgc cgttagtatc attgctaacc ctgatgttta tcatcactct gccgtttacg
     8101 ggcctgagcg cgatttctcg ccacatctcc gccgccgggt tgctgttaac gctggccttt
     8161 ttgcaattga tcttaatggc tattgtccgc gatccgcaaa aagcgtcact tccctggacg
     8221 gggccgttgc gttgcctgat taaaaccgct ttgctggttg ctccgctgta tgtgttcgtc
     8281 gccgcctggg cgttatggct gcgggtcgct cagtacggct ggactgtcga ccgcttgcag
     8341 ggcgcgctgg cggtgctggt gttactggtg tggtcgctgg ggtattttgt cagtatcgtc
     8401 tggcgtaatg ggcaaaatcc ccttgttctt cagggcaaag tgaaccttgc ggtttcgtta
     8461 ttggtgttgg tgatactggt gcttcttaat tcgccggtgc tggacagtat gcgtattagc
     8521 gtgaacagtc atatggcgcg ttatcagagc ggcaaaaaca cgccagacca ggtaactatc
     8581 tacatgctcg aacagagcgg tcgctatgga cgtgcggcgc ttgagtcgct gaaaagcgat
     8641 gccgggttta tgaaagaccc gaaacgcgcg cgggatctgc tgatggcgtt agatggagag
     8701 caacatcttc aggaacaggt atcggaaaaa gtattagccg aaaatgtgtt aattgcccct
     8761 ggttctgtta aaccggatgt gacattctgg tcggccttaa tccaggatcg ctataacgtg
     8821 atgacctgta ttgaaaaaga cgcctgcgtc ctggtcgagc aagatctgaa tagtgatggt
     8881 caggcggagc ggatcctgtt tgcttttaat gatgacagag tcattgtcta tggctttgac
     8941 tcagacagaa aagaatggga cgcgcttgat atgagtttac ttccgaacga aataacgaaa
     9001 gaaaaattac tcacagctgc gaaggatgga aaactgggga cgaggccgaa agcgtggcgc
     9061 gatctgacgg tagatggtga aacgctggaa ataaacttaa gtaagtgaca acgcttttat
     9121 cggatacgac gctgtatctg accaaaataa tttgcgattt atttcatagg cgctggtaag
     9181 cgcagcacat caggccatta agttgtaaaa tccggcagag ccctctctgc cggacatact
     9241 cattaatgaa cttgcggatc tgccggagac gcgttgttgc ggatttcagc aatatccatc
     9301 gcattgaaca gatagtggtt accgcagtaa tcacaatgca tgtcaatttc gccatcttcc
     9361 gccaggatgc tatcaacttc ttcatcaggc agcgttttca gcgcatcggc gcaatgttca
     9421 cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt aaaccgtcac ctcttcttcg
     9481 tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca gcagttcttc ggttttgatg
     9541 gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct gggcattttg cgcaggcatt
     9601 acctgcaaca acataccgcc tgcagccggt ttgccgtcta cgtcgccggt gcgaataaac
     9661 aggcgcgtcg gcagctgttc agaacgcata aagtaatctt ccaggcaggc cgccagggta
     9721 tcaccttcca gaccaactac gccctgatag cgttcgcctt cgctcggggt aatggtgatc
     9781 accacgtaac cattgccgac cagcgttttc aggtcggcat tttctggaat ttcgccctgc
     9841 acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt taataaccgc cagattcatc
     9901 ggaccgtcgc cctgcagctg tacggtgata tcaccatcaa acttcagcgt agcggttaac
     9961 aggctggtcg caaccagcag ttctgccagc acgtttttaa cgggctgcgg ataatcgtgg
    10021 ttctcaagga tctgttgcag ggtttccgaa acggttacca gttcgccgcg aacggcaaaa
    10081 ttttcaaaca gatagcgatg taattggtca tgttgcggca taatcatctc tcttgcaggt
    10141 gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt cgcggcgctc ttttttgtcc
    10201 gggcgtcggt ccgggtgcgg catggttaag gcattaagtt tacgtgccat cgccattttt
    10261 tcgcgtttct ctacactttc cgcagtctct tcatacagca aggctgcctc gctggcgggg
    10321 cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt cgtcatttcc ctggcgcaga
    10381 gtgagcgtgg cattcagctc gacgattttg ctcggcttgc tgcgctgccc gttgtaatgc
    10441 accttaccgc cttcaatcat ttcacgggct agcgcgcggg ttttataaaa acgggcagcc
    10501 catagccatt tatccagtcg aacctcaaca gcaggtttct ctttcatggc gtctccttca
    10561 cattagcgag gggatcaggc ggcggtagtc attcagtgac ggatggcgtt gatactgttt
    10621 ctcggcaatc ccggaatcag gattagtcac gccgaggcag taacgaatac caaattgcgc
    10681 ggcagcatcg agaatcgctt cgctgtcatc aataaacagc gttctttcag ctttcagacc
    10741 cgtagcttcg gccaccgcat gccataaccg ctgatcctct ttcggataac caaatgtgtg
    10801 ggtggaaagt aataaatcaa ggtgtgcgtc cagacccgta tgctcaagtt ttaccgccag
    10861 gttgtgcgga tgagcattgg tgagcaaaat tcgctgctta ccgctggctt tcagtgcctc
    10921 aagaaacgga atggtatctt cacgcagtac ggcgcgcggt cccatctcgg tggtcattgc
    10981 acagatatcc agacccagtt gctcactcca gtaatcaaga cagtaccagt ttagcgtatg
    11041 ctgtacgtcg tgatattgct ggcgcatata ttccatcgct tcctgtggcg taaccccgtt
    11101 tttcgcgccc catgtttcag gcaccagctt ttgccagaaa tagttatcga aggcgaggtc
    11161 gagcaacgtg ccgtccatat ccagcagaac ggtatctacg tcctgccagg caatgttgat
    11221 atgcatgagg gaaatctcca gagtgaagca atttgcgcga cagggtagca taacctgtcg
    11281 cgcaaacgtg ttattcgata aggctttctg aaggggtgat cagttgcggg ttcaggcagc
    11341 tttcataata ggcctgaatc tccatcatgc gagtgcgatg acgctggtaa cgacgccagg
    11401 cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag tagcagcaat gtggtgctga
    11461 gatagcgcca caggccggaa cgatccggga tcggatgaag gccaatatgc tgcgtaccat
    11521 tggcgtcagt gaagattttt gtgacgatac cttcggcgtt aaacggcgta tgcatcagca
    11581 tttgtgccag tttctggaaa gcgttccact gttcttgcgg cgggtagtcg taaagtgatg
    11641 ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc gctgacaatc aggaatccgc
    11701 cgggggccgg actgtttagt gattgtgccg ctcgcgccgt ttcatgcgtg ataaacggcg
    11761 cggtggaggt tgccaccagg ttatccagcg attccgcact caccgggcgt aataacacat
    11821 tcacgccatc gagcttcccg gcgttggcgc gttttaccag cgcgtcccag tctttactgt
    11881 tgccgagatt gaccagcgca tttttcagtc gcacacagtc atctttggca gaacataaat
    11941 ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa taccatgccg gatttttgaa
    12001 tcgctgaacg taatgaggca ctgacgcgag attcatcttc cggtttaggg tgcagctggc
    12061 gattaactgc ttcagtcaat gccgtcgctt tgttgaccag ttcagattct ggtaatggta
    12121 atgagcgggc gtcattccag atgatctgcg agcagtcaaa cggtaaaaaa ggtgaattgg
    12181 ttttcgcgct ccaggttccg gaagtgcgaa tattacacat tcccgtaccg ctaatacgca
    12241 atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt tacgctggtg gcttcaatgg
    12301 tctgcgcgcc tttcatccat gagagagtga atttcagcgg catatccagc gggatccaga
    12361 ataacagcat aaacagcacc agcagcgagc ccgcagcgat aatcgtactg cgcagccagt
    12421 gctgtaacgg aaagtttttt acttcatcat gcagcgaaag atatcgcccc tgacgcacta
    12481 cgtggcagtc gagatagata tcaatatcgg tttgttgatc gagatcctga gcaatgtatg
    12541 gctgccagtg tgcgggatag accaggtcga taataccgag tgaaatattg ttgatctgtt
    12601 cctgatcgtt ttcgccaaac aatccccaac gacggggtgt accgcgcaga caatgaattt
    12661 cccgcaggga ggattttgcc gggggcgcga atagccccca cagacctgcg cccagcagca
    12721 gtaatgcgcc gcctgccagc cacggaacaa atacatccgg ggtaatcagg cagaaaaaga
    12781 acatcaggaa ggaggcgacg atcagcaacg cttcacgcag cccgcgcgga cgactcagcg
    12841 catattcttc atgcgtttct ttgcgaatat tgagtagctc gatttgctca ctctcttccc
    12901 cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc atagccgcgc gtttcctgca
    12961 tgtactcctg tagcgtatga ccgttgagtg aaataaccaa cggcagcgaa tcggtatgaa
    13021 tcagttcaac ggtattctca tcgttgatgt actgttccca gaaggggggc aggtggactt
    13081 ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc ggtagagatg ccgtaacgtg
    13141 tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc attcagcgcc agagagatcg
    13201 gtgccgcgct agctcccgtt gggccaggca cctgtaatac ctgcgtcagg ctctcaagat
    13261 aattttcaac ggcgctacgt tcttcaggtg tgagtttacg cgtttgcgca tccgcgaagg
    13321 cgttggtcca gggcagctga ctccgtctgg atcgcacttt tatcagccat cccgcaagta
    13381 gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt gctcatgctt tccccatctt
    13441 acttagtctg tcaggcgtgg tcagtcgcac cagacacaat acatgattct gcggttggct
    13501 atcggcaagc gtggagttta acttgagcat cattaagcac atcacagtaa ccgctgatca
    13561 tagcaaaccc tctgaaggcg gaatatcagc aaattcctag agttatttca acctattgac
    13621 ttttaatttg atttgaggac gattatttat taagttacat aaatgtaaat aacatgcaat
    13681 tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg ctgatttcac agcatagatc
    13741 ccacgatgag caaatcatta caaaaaccca ccattctgaa tgttgaaact gtagcccgtt
    13801 cccgactgtt taccgtcgag agcgtggatc tggagttcag caatggcgtg cggcgtgttt
    13861 atgaacgaat gcgtccaacc aaccgggaag cagtgatgat tgtgccgatt gtggacgatc
    13921 acctgatcct gatccgcgaa tacgcagtgg gaactgaatc ctacgaatta ggtttttcga
    13981 aaggattaat tgatccgggt gaaagcgtct acgaagccgc taaccgcgag ctaaaagaag
    14041 aggttggatt tggtgcgaac gatctgactt ttttgaagaa gctcagcatg gcaccgtctt
    14101 acttttccag caaaatgaat atcgtggtcg cgcaagatct ctacccggaa tcgctggaag
    14161 gcgatgagcc agagccgcta ccacaggtgc gctggccgct ggcgcatatg atggatttgc
    14221 tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc gctgttcctc gtgcgcgaat
    14281 ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg cctgatgcac aacgcttatc
    14341 aggcttatgg ggttcctgca atatattgat ttttcacgat tttgttagcc gggtaacgcg
    14401 gtcacccgca tccagcataa acaaatgcac tttgtcagcc aactgaaaag gcgccgaagc
    14461 gcctttttaa tcagaacaat tcctgtgcct caccattatc gataatggtc gttcccacct
    14521 cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa atactcttcg cggctgttgc
    14581 caccattagc taactgcccg gtgctgcgat cgatattcac cgtcacaata cccggtggcg
    14641 gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt catataagcg tcccatgcag
    14701 gctgggcact cttggctccg ccttcgtaac ctgagatctg atctttaatc gctccggaag
    14761 ccgttgtatg accgagatta cgacggtgat catcaaagcc aatccagacc gaggtcacaa
    14821 cgcccggacc gtaacccgag aaccacgcat ctttcgaact gttagtggta ccggttttcc
    14881 cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca gccagtaccc tgccagcctg
    14941 gctcaccaaa gatattggtg ttcaaagcac tcttaatcag gaatgccagc ggagtgttga
    15001 tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac taacgcctga tttgcctgct
    15061 ccagctgcgg cattggtaca gaaacattct gctgctcgcg ggagatagcg acatcttcaa
    15121 catcgttatt ttccagcacg ttcgatttct gcgtatcacc gtaaatcacc ggaatatcgc
    15181 attccgggca ggctactttc ggtttcgctt cgaaaatcac gccgccctga tcgttttcaa
    15241 ttttgctgat aaaccacggg tccaccagga agccgccgtt cgccatgacc gcgtagccgc
    15301 gcgccacctg cattggggtg aaggacgctg aacccagcgc cagcgattcg gtgtggacaa
    15361 tgttttgcgc cgggaagccg aagcgttgca gatattctgc agcgtagtcg acgcccatag
    15421 cccgcattgc acgtaccatc accacgtttt tcgactgacc cagcccctga cgtaagcgaa
    15481 ttggaccagc atattgtggc ggcgagtttt tcggctgcca gtcagaaccg gcacctgcgt
    15541 cccagcgaga aattggcaca tcgttcaaca tacttgccag cgtcagacct ttatccatcg
    15601 ccgcggtgta gaggaacggt ttgatgttgg aacccacctg acgcagtgcc tgggtggcgc
    15661 ggttaaactt gctctgattg aaatcaaagc caccgaccag cgccataacg gcaccgtttt
    15721 gcggattgat cgacaccagc gccgagttca cttccggcac ttgtgccagc caccatgcat
    15781 cgccaacctg acgaacccag atttgctgac ccgtttgcag aacatcggtc actttacgcg
    15841 gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc ccagcgaacg ccttccatac
    15901 tcaatgcgac ggtcgacccg tccgccagca tcgccgtcgc tccctgagga ttggcgctgg
    15961 tgactgcggc aggcagcagc ggaccatagg tcggcagcgc cttcagcgta tcggtaatct
    16021 tgttgttatc ccacgccgac tcgcccactt tccacagcac atttgccggg ccgcgatagc
    16081 cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc ctgctgtgcg gcctgctgca
    16141 ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc ataggcactt tcgccataac
    16201 ggttatacat ctcctggcgc accatttcgc tcaggtacgg cgcagagaaa gcaatctccg
    16261 gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg atcgaactgt tgttgggtga
    16321 tatacccttc atccagcatc cgcgacagta cgacgttacg ccgcgcgacg gcacgatcca
    16381 ttgagtagag cggattaaag gtggaaggcg ctttcggcag cccggctatc acagccattt
    16441 cgttcagcgt cagttggtcg accgttttac cgaaatagac ttgtgccgca gcaccgacac
    16501 cataggcgcg gtaaccgagg taaatcttat tcagataaag ctcgaggatc tcgtctttcg
    16561 tcagcaactg ttcaatgcga atcgcgagga agacttcctt aatcttacgc atcagcgtgc
    16621 gttctggact gaggaagaag tttctcgcca gctgctgggt aatggtactc gccccttgtg
    16681 acgcgtggcc ggagaacagc gccacacttg ccgcacggaa gatccccacc gggtcaacgc
    16741 cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa ggctttcacc atctccggcg
    16801 ggatttgatc caacgtaacc ggaatacgac gtttctcacc gtattgagca atcagctcgc
    16861 catcggcgct gtaaatctgc atcggaattt gcaggcgaac atcttttaat gtcgccacat
    16921 ccggcagttg tggctcgatg tagcggtata ggccataaat cgagcctgct cccagcagaa
    16981 tgcaacagac tgcaaggatc aaaaaatact ttacgaactt cactggaaat ttcccattta
    17041 gtttcatttg ggcagtttat aaacaaacgc gcgttagtat aaaggcaagc cagacgcatt
    17101 gatatacccg tcagagtgac gggtgataag gagatcatca caatggcatt taagatctgg
    17161 caaattggtt tgcatttaca acagcaagaa gcggtagcgg ttgcgatcgt gcggggcgca
    17221 aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg agaacgacat tatcaaagat
    17281 gggcggattg ttgatgcgca gcagctggct aaaacgttgt taccttggag tcgcgaactg
    17341 ccgcagcgtc atcacattat gttggcgttt cccgccagtc gcacattaca gcggtcattt
    17401 ccgcgcccgt cgatgtccct tggtgagcgg gagcaaacgg cctggctgtc agggacgatg
    17461 gcccgcgagc tggatatgga tccggactcc ctgcgcttcg attacagcga agactcactc
    17521 agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc tggcaacgct gcttactctg
    17581 gcagaaaggt tgcgtgttca tgttagtgcg atcaccccgg atgccagtgc attacagcga
    17641 ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct ggcgtgataa cgaacagtgg
    17701 ctgtgggcga cgcgctatag ctgggggcgc aaactggcgg tggggatgac tagcgcgaag
    17761 gagctggcgg cagcgttatt cgttgatccc gacagcgtcg cgatatgtgg cgaaggcgga
    17821 tttgacccct gggaggccgt ttctgttcgt cagccgctgc taccgccgcc tggtggagac
    17881 tttgccatcg cgctggggct ggcgcttggg aaggcgtact gatgaacccg ccaattaatt
    17941 ttttgccctg gcgacagcaa cgccggaccg cttttctgcg tttctggttg ctgatgttcg
    18001 ttgcgcctct gctgctggcc gtcgggataa cgctaatact gcgtctgaca ggcagcgccg
    18061 aagcgcgcat agacgcagtt ttgcttcagg cggaacaaca actcgcccgc agcttacaga
    18121 taacgaagcc acgtttgctg gagcaacagc agttacgcaa acagcgttcg caacggcagc
    18181 gccagcgaca atttacccgc gactggcaat ctgcgctgga agcactggcg gcgcttttac
    18241 ccgagcacgc ctggctgaca acgataagct ggcagcaggg aacgctggag atcaaggggc
    18301 tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc actccgccag gatgcgtctt
    18361 ttcatctcaa tcagcgggga gccacgcagc aggatgcgca gggacgctgg caatttgagt
    18421 atcagttaac aaggaaggtt agcgatgaac atgttctttg actggtggtt cgccacatca
    18481 ccccgcctcc gccagttttg ctgggcattc tggttgctga tgttagttac gctcattttt
    18541 ctgtcatcga cacaccatga agagcgcgac gcattaattc gattacgggc aagtcatcac
    18601 cagcagtggg ccacactgta tcgcctggtt gacaccactc ccttcagcga agaaaaaacg
    18661 ctgccctttt cgccactgga ttttcagttg cccggcgcgc aactggtttc ctggcatcca
    18721 tccgcgcagg gaggtgagtt ggcgttgaaa acgctgtggg aagcagtgcc gtcggcattc
    18781 acacggctgg cagagcgcaa cgtcagcgtg agccgttttt cgttaagcgt ggaaggtgat
    18841 gatcttttgt tcacgctaca actggagacg ccgcatgagg gttaaacgct ggttgttggc
    18901 aggtattgca ttgtgccttt taaccggtat gcgtgaccct tttaaaccgc cggaagatct
    18961 atgccggatt agcgaactta gccagtggcg ctatcagggg atggtagggc gaggcgagcg
    19021 catcatcggt gtaataaaag acgggcaaaa gaaatggcga cgggtgcagc aaaacgatgt
    19081 gctggaaaac ggctggacaa ttttacagct gacgccagac gtactaacgc tgggtaccgg
    19141 gacaaactgc gaaccgccac aatggttgtg gcaacggcaa ggagatacaa atgaagcaat
    19201 ggatagccgc actactgttg atgctgatac ccggcgtaca ggcggcaaag ccgcaaaaag
    19261 tgacgctgat ggtggatgac gttccggtag ctcaggtgtt gcaggcgctg gctgaacagg
    19321 agaagttgaa cctggtcgtg tcgccagacg tcagcggtac ggtgtcgtta catctaacag
    19381 atgttccctg gaagcaggca ctacaaactg tagtgaaaag cgccggactg ataacgcggc
    19441 aggaaggcaa cattctctca gtgcattcca ttgcctggca gaataacaat atcgcccgcc
    19501 aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct ggaaaatcgc agtataaccc
    19561 tgcaatacgc cgacgcggga gaactggcga aagcggggga gaagctactg agtgccaaag
    19621 ggagtatgac cgtcgataaa cgcaccaatc gccttttgct acgagataac aaaacggcgt
    19681 taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc ggtcgggcag gttgagctgt
    19741 cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga gttaggcgtg aaatggacgc
    19801 tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac cacgcttggt agcgacctct
    19861 ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg gcgcatcaac ggacgcttgc
    19921 tggatcttga gctttccgcg ctcgaacaaa aacagcagct ggatattatc gccagtccgc
    19981 gtctgctggc ctcacatctt cagcctgcca gcattaaaca ggggagcgaa attccatatc
    20041 aggtttccag cggggaaagt ggcgcgacgt cggtggaatt taaagaggcc gtcctgggga
    20101 tagaggtcac gcccacggtg ttacaaaaag gtcgcatccg gctgaaatta cacatcagcc
    20161 agaacgttcc ggggcaggtg ctacagcagg ccgatggcga agtgctggcg attgataagc
    20221 aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac gttggcgctg ggcggcattt
    20281 ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt gcttggcgac attccctggt
    20341 tcgggcaatt atttcgtcat gacggaaaag aatatgaacg acgcgagtta gtggtgttta
    20401 tcacgccacg actggtttcc agtgagtaaa cagccgtaaa agcggtaatg tttttatgct
    20461 gaacgtgttt catctatttg acgcgcgcag gtatttagca tacaaggagt accgatttga
    20521 gagttggcgc tcttcgctgc ctgcgttcca tgatgatgat ttatcattca ggcggcattt
    20581 tgctgtcttt tttacgctaa tcttacccgg tgatttatcg ccagagcggt ggtagcaagg
    20641 cagcgcgctt gcagcgacca gatatgcaga gggatgggtg atttattcag ttgccaaacc
    20701 cgctggagta ttgagataat tttcagtctg actctcgcaa tatcttatga ggtttcagtt
    20761 catgtcctgc ggcgctctct gagcgaagcg ggtttatcat taacgaatag tcttagtagt
    20821 accgaaaaaa tggcagagaa acgcaatatc tttctggttg ggcctatggg tgccggaaaa
    20881 agcactattg ggcgccagtt agctcaacaa ctcaatatgg aattttacga ttccgatcaa
    20941 gagattgaga aacgaaccgg agctgatgtg ggctgggttt tcgatttaga aggcgaagaa
    21001 ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga ccgagaaaca gggtattgtg
    21061 ctggctactg gcggcggctc tgtgaaatcc cgtgaaacgc gtaaccgtct ttccgctcgt
    21121 ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac ttgcacgcac gcagcgtgat
    21181 aaaaaacgcc cgttgctgca cgttgaaaca ccgccgcgtg aagttctgga agcgttggcc
    21241 aatgaacgca atccgctgta tgaagagatt gccgacgtga ccattcgtac tgatgatcaa
    21301 agcgctaaag tggttgcaaa ccagattatt cacatgctgg aaagcaacta attctggctt
    21361 tatatacact cgtctgcggg tacagtaatt aaggtggatg tcgcgttatg gagaggattg
    21421 tcgttactct cggggaacgt agttacccaa ttaccatcgc atctggtttg tttaatgaac
    21481 cagcttcatt cttaccgctg aaatcgggcg agcaggtcat gttggtcacc aacgaaaccc
    21541 tggctcctct gtatctcgat aaggtccgcg gcgtacttga acaggcgggt gttaacgtcg
    21601 atagcgttat cctccctgac ggcgagcagt ataaaagcct ggctgtactc gataccgtct
    21661 ttacggcgtt gttacaaaag ccgcatggtc gcgatactac gctggtggcg cttggcggcg
    21721 gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta tcagcgcggt gttcgtttca
    21781 ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc cgttggcggc aaaactgcgg
    21841 tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta ccagcctgct tcagtggtgg
    21901 tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt agcgtcgggg ctggcagaag
    21961 tcatcaaata cggcattatt cttgacggtg cgttttttaa ctggctggaa gagaatctgg
    22021 atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg tattcgccgt tgttgtgaac
    22081 tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg gttacgtgct ttactgaatc
    22141 tgggacacac ctttggtcat gccattgaag ctgaaatggg gtatggcaat tggttacatg
    22201 gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac gtcggaacgt ctcgggcagt
    22261 ttagttctgc cgaaacgcag cgtattataa ccctgctcaa gcgggctggg ttaccggtca
    22321 atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca tatgctgcgt gacaagaaag
    22381 tccttgcggg agagatacgc ttaattcttc cgttggcaat tggtaagagt gaagttcgca
    22441 gcggcgtttc gcacgagctt gttcttaacg ccattgccga ttgtcaatca gcgtaacaac
    22501 aagaaaggtc aggccgctta tcaagcggtc tattagcttc aggttaattg caacgtggta
    22561 agcattaacc ttttagtggg gtgttaaatg gatgaattca aaccagaaga cgagctgaaa
    22621 cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat cttctgaacg ttctgagcgt
    22681 actgaacgtg gcgaaccgca gatcaatttt gatgatattg aacttgatga cactgacgat
    22741 cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac cggaaatcga agaagaaatt
    22801 gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag agcgtcgtcc gcgtaagcgc
    22861 aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga tgatgggcgt cggcattctg
    22921 gttctactgc tgttgatcat cggtatcggt tctgcgctaa aagccccctc gacctcttcc
    22981 agcgatcaaa ccgcgtctgg cgataagagt attgatcttg caggcaatgc gaccgatcag
    23041 gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg aaaatactca gcaggatgtt
    23101 tctctgccac cgatctcttc tacgccgact caagggcaaa ccccggcggc aacggatggt
    23161 caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc tgactcagcc acaaaatcag
    23221 caacagttga acaatgtggc ggtcaattcc acattgccga ccgaacccgc gactgtcgcg
    23281 cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa cgcaaaccgc tgaacgtccg
    23341 tccactacgc gcccagctcg tcagcaggcg gtgattgaac cgaaaaaacc gcaagcaacc
    23401 gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc gtactgaacc agctgcccct
    23461 gtggcgagca cgaaggcacc ggctgcgact tctacgccag caccaaaaga gacggcgact
    23521 acggctccag tacagacggc atccccggcg caaaccacgg caacaccagc cgctggaggg
    23581 aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt ccagccatta cactctgcag
    23641 ctgagcagtt cctctaacta cgacaacctg aacggttggg cgaagaaaga gaatctgaaa
    23701 aactacgttg tctatgaaac gacgcgtaat ggtcagccgt ggtatgtcct ggtttctggc
    23761 gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta cattgccagc agatgttcag
    23821 gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg ccgatctgaa gtaatcaagg
    23881 ttatctcccg caatggttta tcgttgcggg agctgcctga agcgctggat gctgtcggag
    23941 ctttctccac agccggagaa ggtgtaatta gttagtcagc atgaagaaaa atcgcgcttt
    24001 tttgaagtgg gcagggggca agtatcccct gcttgatgat attaaacggc atttgcccaa
    24061 gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg gtgtttctca acaccgactt
    24121 ttctcgttat atccttgccg atatcaatag cgacctgatc agtctctata acattgtgaa
    24181 gatgcgtact gatgagtacg tacaggccgc acgcgagctg tttgttcccg aaacaaattg
    24241 cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa agccaggatc cgttccgtcg
    24301 ggcggtactg tttttatatt tgaaccgcta cggttacaac ggcctgtgtc gttacaatct
    24361 gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa ccctatttcc cggaagcaga
    24421 gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc tattgtgagt cttacgccga
    24481 tagcatggcg cgcgcagatg atgcatccgt cgtctattgc gatccgcctt atgcaccgct
    24541 gtctgcgacc gccaacttta cggcgtatca cacaaacagt tttacgcttg aacaacaagc
    24601 gcatctggcg gagatcgccg aaggtctggt tgagcgccat attccagtgc tgatctccaa
    24661 tcacgatacg atgttaacgc gtgagtggta tcagcgcgca aaattgcatg tcgtcaaagt
    24721 tcgacgcagt ataagcagca acggcggcac acgtaaaaag gtggacgaac tgctggcttt
    24781 gtacaaacca ggagtcgttt cacccgcgaa aaaataattc tcaaggagaa gcggatgaaa
    24841 cagtatttga ttgccccctc aattctgtcg gctgattttg cccgcctggg tgaagatacc
    24901 gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg acgtcatgga taaccactat
    24961 gttcccaatc tgacgattgg gccaatggtg ctgaaatcct tgcgtaacta tggcattacc
    25021 gcccctatcg atgtacatct gatggtgaaa cccgtcgatc gcattgtgcc tgatttcgct
    25081 gccgctggtg ccagcatcat tacctttcat ccagaagcct ccgagcatgt tgaccgcacg
    25141 ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg tatttaaccc ggcgacacct
    25201 ctgagctatc tggattacgt gatggataag ctggatgtga tcctgctgat gtctgttaac
    25261 ccaggttttg gcggtcagtc gttcattcca caaacactgg ataaactgcg cgaagtacgt
    25321 cgtcgtatcg acgagtctgg ttttgacatt cgactggaag tggacggtgg cgtgaaggtg
    25381 aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt tcgtcgccgg ttcggcaatc
    25441 ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc gcagtgaact ggcaaaggta
    25501 agtcatgaat aagtttgaag atattcgcgg cgtcgctttt gatctcgacg gtacgctggt
    25561 tgacagtgct cccggtcttg ctgctgcggt agatatggcg ctgtatgcgc tggagttacc
    25621 cgtcgcaggt gaagaacgcg ttattacctg gattggtaac ggcgcagatg ttctgatgga
    25681 gcgtgcattg gcctgggcgc gtcaggaacg tgcgactctg cgtaaaacaa tgggtaaacc
    25741 gcccgttgat gacgacattc cggctgaaga acaggtacgt attctgcgta aactgttcga
    25801 tcgctactat agcgaggttg ccgaagaggg gacgtttttg ttcccgcacg ttgccgatac
    25861 gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg gtcaccaaca aaccgacgcc
    25921 gttcgtcgcg ccactgctcg aagccttaga tattgccaaa tactttagcg tggttatcgg
    25981 cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg ctgttactgg tggctgagcg
    26041 gatgggaatt gccccacaac agatgctgtt tgtcggcgac tcacgcaatg atattcaggc
    26101 ggcaaaagcg gcaggttgcc catcagttgg cttaacctac ggatataact acggcgaggc
    26161 tatcgatctc agccagcctg atgtaattta tcagtctata aatgaccttc tgcccgcatt
    26221 agggcttccg catagcgaaa atcaggaatc gaaaaatgac taagcccatc gtttttagtg
    26281 gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat gggtgcgctg cgtcagtggg
    26341 taaacatgca ggatgactac cattgcattt actgtatcgt tgaccaacac gcgatcaccg
    26401 tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga tacgctggcc ttgtatctgg
    26461 cttgtggtat cgatcctgag aaaagcacca tttttgttca gtcccacgta ccagaacatg
    26521 cgcagttagg ctgggcactg aactgctata cctacttcgg cgaactgagc cgcatgaccc
    26581 agtttaaaga taaatctgcg cgttatgccg agaacatcaa cgctggtctg tttgactatc
    26641 cggtgctgat ggctgcggac atcctgctgt atcaaactaa tctggtaccg gtgggtgaag
    26701 accagaaaca gcacctggaa ctgagtcgcg atatcgccca gcgtttcaac gcgctgtatg
    26761 gcgagatctt taaggtgccg gagccgttta ttccgaaatc tggcgcgcgc gtaatgtcgc
    26821 tgctggagcc gaccaagaag atgtccaagt ctgacgataa ccgcaataac gttatcggcc
    26881 tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg cgcggtcact gactccgacg
    26941 agccgccggt agttcgctac gatgtgcaga acaaagcggg cgtttccaac ctgctggata
    27001 tcctttctgc ggtaacgggc cagagcatcc cagaactgga aaaacagttc gaaggcaaga
    27061 tgtatggcca tctgaaaggt gaagtggctg atgccgtttc cggtatgctg actgaattgc
    27121 aggaacgcta tcaccgtttc cgcaacgatg aagccttcct gcaacaggtg atgaaagacg
    27181 gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa agcggtgtac gaagcgattg
    27241 gttttgtggc gaagccgtaa tatcaaaacg ctcggacggt cccctcgccc ctttggggag
    27301 agggttaggg tgaggggagc aggcaagcgc cagtgggaat taccctttgc tcgcattgtc
    27361 gaataaatca cttcgtcatc actctgaaac cggggaaacc cggttttttt atcctcattt
    27421 gcaataatcc gaaaaaatgt gaagcgcctc gccgtttcca catctcacag ttgccactta
    27481 tcttcatttt aatgaagata taaacattca ttttattgaa aatatattat gcgtcgaacg
    27541 tttatcaaaa aagaaggcgt cgtcatcacg acgctggccc gttatttgtt gggtgaaaaa
    27601 tgcggtaatc gactgaaaac catagatgag ctggcaactg aatgccgttc atcggttggc
    27661 ctgacgcagg ccgcgttgaa aacgttggaa tcaagtggag cgatacggat tgaacgccgt
    27721 gggcgcaatg gcagttatct ggtcgaaatg gataacaaag cgctgctgag tcatgtggat
    27781 atcaataacg ttgtgtgtgc catgcctttg ccctataccc gcttgtacga aggtttagca
    27841 agcgggctga aagcccagtt tgatggcatt cctttttatt atgcgcatat gcgtggcgcg
    27901 gatattcgtg tggagtgttt gcttaacggt gtctatgaca tggcagtagt ttcccgcctg
    27961 gcggcggaaa gttatctcag ccaaaacaat ttgtgcatcg ccctcgagct tggcccgcat
    28021 acgtatgttg gtgaacatca actgatatgc cgcaaaggtg agtctggaaa tgttaagcga
    28081 gtaggtctcg atagtcgttc ggcggatcag aaaatcatga ccgatgtctt ttttggcgat
    28141 agtgatgtgg aacgtgtcga tctctcttat cacgagagtt tacaacgcat tgttaaaggc
    28201 gatgtcgatg cggttatctg gaacgtggtg gcggaaaacg aactgaccat gctgggatta
    28261 gaggcgacgc cgctcacaga cgatccgcga tttttacagg ccaccgaagc cgtggtcctg
    28321 acgcgagtcg atgattaccc aatgcaacaa ctgctgcgtg ccgttgtaga taaacacgcc
    28381 ctgctcgccc atcaacaacg ggtagtgagt ggggaacagg aaccgagtta ttaaacgaaa
    28441 ggcatctgat atggaaacca gactcaacct gctttgcgag gcaggcgtta ttgataagga
    28501 catctgcaaa ggcatgatgc aggtcgtcaa cgtactggaa acagagtgcc atctgccggt
    28561 gcgcagtgag caaggaacga tggcgatgac acatatggcg agtgcactga tgcgcagtcg
    28621 ccgtggtgaa gaaatagagc cgctggataa cgaattgctg gcagagctgg cgcaatccag
    28681 ccactggcaa gccgttgtgc aattgcatca ggtgttgttg aaggaattcg cactggaagt
    28741 taatccgtgt gaagaaggct atttgctggc caacctttat ggattatgga tggctgctaa
    28801 cgaagaggtt tgaagctccc gacaaatcca cgtcgcacaa cctaaatatt caaaaaaatg
    28861 aatatttaat tcaaggaaca gaaacgaggc aacaagatgt ttgtagaagc attgaaacgc
    28921 cagaacccgg cgctgatttc cgccgcacta agtttgtggc agcagggcaa gatcgccccg
    28981 gacagctggg tgatcgacgt ggatcaggta ctggaaaacg gtaagcgact gattgagacg
    29041 gcgcggcttt acggcattga actgtatctg atgaccaagc agtttggccg taacccatgg
    29101 ctggcggaaa aattgctggc attaggttac agcggcattg tggcggtgga ttacaaagag
    29161 gcgcgagtca tgcgccgcgc tggtttgtcg gtggcgcatc aggggcatct ggtacaaatc
    29221 ccttgtcatc aggttgctga cgccgttgaa cagggcaccg acgtcatcac cgtgtttact
    29281 ctcgacaaag cgcgggaagt ttctgcggca gcggtgaagg ccggacgtgt gcagtttgtg
    29341 ctgctcaaag tttatagcga cgatgatttt ctttatccgg ggcaggagag cggttttgta
    29401 cagcattcgc tgcacgaggt ggttgctgaa attaagaaac tgccgggtct gcatttagcc
    29461 ggacttaccc atttcccttg cctgctttgg gatgaggctg ccggaaaagt tttgccgaca
    29521 ccgaatcttc acacgctgat acaggcacgg gatcaactgg cgaaatctgg tattgcactt
    29581 gagcaactga acgcgccttc agcgaccagc tgcacttcgc tgccattact ggcgcaatac
    29641 ggtgtgactc atgccgaacc cggtcatgcg ctgacgggca ctattccggc aaaccagcag
    29701 ggcgatcagt ctgaacgtat cgcgatgctc tggttgagtg aaatctccca tcatttccgt
    29761 ggcgacagct actgctacgg cggcggttac taccgtcgtg gtcatgcgca acatgcgttg
    29821 gtgtttacgc cagaaaatca aaagattact gaaaccaatc tcaaaactgt ggatgacagc
    29881 agtatcgact acaccctgtc gctggcaggc gagtttccgg taagcagtgc agtggtgctc
    29941 tgtttccgca cgcagatttt tgtcacccgt agcgatgtgg tgctggtgtc cggtattcac
    30001 cgtggcgaac cgaaaatcgt tggtcgttat gacagtcttg gaaactctct gggggcgtaa
    30061 tggcgcgatt tgtggtgtta gtgattgata gctttggcgt aggggcaatg aaagatgtca
    30121 cgctggtgcg tccgcaagat gcgggagcga atacatgtgg tcacatcctg agccagttgc
    30181 cgcatttgca gctaccaacg ctggagaagc tggggctaat caacgcattg ggttatgcgc
    30241 caggcgatat gcagccgtca gattccgcaa cctggggcgt ggcagagctg caacatgaag
    30301 gtggcgatac ctttatgggg catcaggaaa ttttaggcac gcgcccgtta ccgccgctgc
    30361 ggatgccttt ttgcgatgtg attgaccgtg ttgagcaggc attagtatcc gccggttggc
    30421 aggtggagcg ccgtggcgat gaactgcaat ttctgtgggt caatcaggcg gttgcgattg
    30481 gcgataatct cgaggcggat ttaggccaag tctataacat taccgccaat ctctctgtga
    30541 tctcttttga cgacgcaatc aaaattggtc gtatcgtgcg tgagcaggta caggtcggtc
    30601 gggtcattac atttggtggc ctgttaaccg acagtcaacg cattctcgat gccgcagaaa
    30661 gcaaagaagg gcgctttatt ggtatcaatg cgccgcgttc tggcgcttat gacaacggct
    30721 tccaggtcgt gcatatgggc tacggtgtcg ataaaaaagt gcaggtgccg caaaaactgt
    30781 atgaggcggg cgtgccaacc gtgctggtgg gtaaggtggc agatatcgtc agcaatcctt
    30841 atggcgtgag ctggcaaaac ctggtggata gccagcggat tatggatatc accctcgacg
    30901 aatttaacac ccatccgacg gcgtttattt gcaccaatat ccaggaaacc gacctcgctg
    30961 gtcatgcaga agacgtcgca cgttatgccg aacgtttgca ggtcgttgac cgtaaccttg
    31021 cccggcttgt tgatgcgatg cagccagatg attgcctggt cgtgatggcg gatcacggca
    31081 acgatccgac cattggtcac agccaccata cccgcgaagt ggtgccgctg ctggtttatc
    31141 agcaaggcct ggctgctacg cagctcggtg tgcgcaccac gctttctgat gtgggagcta
    31201 ccgtgtgtga atttttccgt gcgccaccgc cacaaaatgg tcgctctttt ctttcctccc
    31261 tccggtttgc aggagacacc ctatgagttt tgatccgacg ggttacaccc tggcccatga
    31321 gcatctgcat attgatctct ccggctttaa aaacaacgtg gactgccgcc ttgatcagta
    31381 tgcgttcatt tgccaggaga tgaacgacct gatgacccgg ggtgtacgta atgtgattga
    31441 gatgaccaac cgttacatgg ggcgcaatgc gcaatttatg ctcggtgtaa tgcgcgaaac
    31501 cgggatcaac gtggtggcct gtactggtta ttaccaggat gcgtttttcc cggaacatgt
    31561 ggtgacccgc agcgtgcagg aactggcgca ggagatggtc gatgaaattg aacagggtat
    31621 cgatggcacg gagctgaaag ccgggctcat cgcggagatc ggctccagcg aaggaaagat
    31681 tacgccgctg gaagagaagg tatttattgc cgctgcgctg gcgcataacc agaccggacg
    31741 cccgatctcc acgcatacgt cgttcagcac catggggctg gaacaactgg ctctgttgca
    31801 agcccacgga gttgatcttt cgcgcgtcac cgttggtcac tgcgatctga aagacaacct
    31861 cgacaacatt ttgaagatga tcgatctcgg cgcgtacgtg cagttcgaca ccattggtaa
    31921 gaacagttac tacccggacg aaaagcgcat tgcgatgctc cacgcgctac gtgaccgtgg
    31981 gttgctgaac cgcgtcatgc tgtcgatgga tattacgcgc cgctcccatt taaaagccaa
    32041 tggtggttat ggctatgact atttattgac cacctttatt ccgcaattgc gccagtcagg
    32101 attcagtcag gccgatgtgg atgtgatgtt acgtgaaact ccctctcaat ttttccaata
    32161 aggacagact catgaaaaag attggcgttg caggcttaca gcgtgagcag attaaaaaaa
    32221 ccattgaagc gacggctcct ggcagttttg aagttttcat tcacaacgac atggaagcgg
    32281 caatgaaagt gaaatccggg caactggatt actacatcgg tgcgtgtaat accggtgcgg
    32341 gcgcggcatt gtcgattgcc atcgcggtga ttggctataa caaaagttgc accattgcca
    32401 aaccaggcat taaagcgaaa gacgagcata tcgccaaaat gatcgctgaa ggaaaagtgg
    32461 cgtttggcct ttccgttgag cacgtcgaac acgcgattcc gatgctgatt aaccatctga
    32521 aataaaaggc acgactatgg atctgtatat tcagattatc gtggtggcgt gcctgacggg
    32581 tatgacatcg cttctggcgc atcgctcggc ggctgttttt catgacggca tccgcccgat
    32641 cctgccgcaa ctgattgaag gctatatgaa ccgtcgcgag gcggggagta tcgcttttgg
    32701 tctgagcatt ggttttgtgg cctcggtggg gatctctttt accctgaaaa ccgggctgct
    32761 caacgcatgg ttactctttc ttcctaccga tatcctcggc gtactggcga taaacagcct
    32821 gatggcgttt ggtcttggcg ctatctgggg cgtgttgatc cttacttgcc tgttgccagt
    32881 aaaccagctg ctgaccgcgc tgccggtgga tgtattaggt agcctcgggg aattaagctc
    32941 gccggtagtt tctgcttttg cactgttccc gctggtggcg attttctacc agtttggctg
    33001 gaagcaaagt ctggtcgccg ccgtggttgt tctgatgacc cgtgtggtag tcgtgcgcta
    33061 tttcccacat cttaaccctg aatccatcga aatctttatt ggcatggtga tgctgctggg
    33121 gatcgcgata actcacgacc tgcgtcatcg tgatgaaaat gatattgatg ccagcgggct
    33181 ttcggtgttt gaagaacgca cgtcgcggat tatcaaaaac ttaccctata tcgccatcgt
    33241 gggagcattg attgccgccg ttgccagcat gaagattttc gccggcagtg aagtgtcgat
    33301 cttcactctg gagaaagcgt actccgcagg cgtaacgccg gaacaatcgc aaacgctgat
    33361 caaccaggcg gcactggcag aatttatgcg cgggctgggt tttgtaccgt tgattgccac
    33421 caccgcctta gcaacgggtg tgtatgcagt tgcgggcttt acctttgttt atgcggtggg
    33481 ctatctctcg ccgaatccga tggttgcagc ggtattaggc gcagtggtta tttcggcgga
    33541 agtcttgctg cttcgttcta tcggcaaatg gctgggacgc tacccgtcgg tgcgtaatgc
    33601 gtcggataac atccgtaacg ccatgaatat gctgatggaa gtggcgctgc tggtcggttc
    33661 gattttcgca gcaattaaga tggcgggtta taccggattc tctatcgcgg ttgccattta
    33721 cttcctcaac gaatccctgg gccgtccggt acagaaaatg gcggcaccgg tcgtggcagt
    33781 aatgatcacc ggtattctgc tgaatgttct ttactggctt ggcctgttcg ttccggctta
    33841 aggaaggcac ctatgaagac gtttcctctg caaagcctga cgcttattga ggcgcagcaa
    33901 aagcagtttg cgctggtgga cacgatttgc cgtcactttc ccggcgcgga gtttctaacc
    33961 agcggtgatt tgggcttaac gccggggctg aatcaaccgc gtattaccca acgggtggag
    34021 caggtgctgg ctgatgcatt tcacgcacag gctgcggcgc tggtgcaggg cgcggggact
    34081 ggcgcgattc gcgccgggct ggcagctttg ctcaaaccgg ggcagcgtct tctggtgcat
    34141 gacgcgcctg tttacccgac gacacgggtt attattgagc agatggggct gacgcttatt
    34201 actgttgatt tcaatgacct gtcggcactg aagcaggtcg tcgacgagca acaaccggat
    34261 gcggcgctgg tgcagcatac gcgccagcag ccgcaggaca gctacgtgct ggcagatgtg
    34321 ctggcaacgt tgcgcgcggc aggtgttcca gcgttaaccg atgacaacta tgcggtgatg
    34381 aaggtggcgc gaatcggctg tgaatgcggc gcgaatgtct cgacattttc ctgcttcaag
    34441 ctatttgggc cagagggtgt tggtgcagtg gtcggcgatg ctgatgttat caaccgtatt
    34501 cgcgccacgc tttactccgg cggtagccag atccagggcg cacaggcgct ggaagtattg
    34561 cgttgtctgg tgtttgcgcc agtgatgcac gcggtgcagg caggggtatc tgaacggttg
    34621 ctggctttgc ttaacggtgg tgaggtgccg gaagtgaaaa gcgcggtgat tgctaatgcg
    34681 cagtcgaagg tgttgattgt cgagtttcat cagccgattg ccgccagagt gctggaagag
    34741 gcgcaaaagc gcggtgcctt gccttacccg gtgggtgcag agtcgaaata tgaaatcccg
    34801 ccgctctttt atcgcctttc cggaacgttt cgccaggcga atccacaatc agaacattgt
    34861 gcgattcgca ttaacccgaa tcgcagcggt gaagagacgg tgctgcggat tttgcgtgag
    34921 agtattgcca gtatttaatg ctggatagca ccgtcaagct aaattccgta ctgaacggtc
    34981 ccctcgcccc tttggggaga gggttagggt gaggggaacg tacgggatgt aaaattcaac
    35041 gcgccagcat cgccggatgc gatgctggcg catcttatcc ggcctacggt taatgtcttt
    35101 tattatcaat agttaaggtg atgcgattgg cgcggcagaa gagttcggag acgtgcaccg
    35161 gtttgtcatt ctgatcaaag gcgattttgc tgatgcgaaa caacggttcg cccagttcgc
    35221 atttcagcca ttttgcctgc gggcgggtgg cggcaaagat atcgatggtc tttttgtcgc
    35281 tgaccactcg cgtgtcaaaa cgctcctgaa ataactgata ggtggagctt ccttcgacgt
    35341 aaatctcatc aaagtcagga taacgcgaca gcgggatcca tgaactatca ataaacagcg
    35401 gctctttatc gagatacatc acccggcaga gatggaacac ttcgctgttt ccggggatgt
    35461 tcagcttttt gcaaaacggc gcggcgctga cccgttcctg ttcgatcact ttctctttcg
    35521 tcgccttgcc ttgtgagacg ccaaaatcgg taaaaccact gacagtaagc agggcgtttt
    35581 caactttctg gctttgtaca aaggtgcctt ttccctgcca gcggatcagt acgccgtccg
    35641 ccactaagtc gctgatggct ttgcgaatgg taatgcggct gacgttatat tgtgtacaaa
    35701 gctcgttttc ggtagggatc tgttgcccgg cctggtaaac cccctgcgcg atatcatcca
    35761 gcagtcgctg gcggacggta gcgtagagga gttgatgaga gtagcggtcc gtagctgaca
    35821 taatcgaccc tgaaataaag aatgaattac tcggattata gggtcgtttt ccggcaagta
    35881 acagcacgtt ggcgtgaaga tgctcacgcc aacgttatac ctaccaggca ccgtggtact
    35941 gaatggtttt cgccgcgcac gccgttccct gcgccatcgc ctgcggtaat gtcatccccg
    36001 cagaccagcc gcaaaggaat ccggcaatga acgaatctcc ggcacccatg gtgtcgataa
    36061 ccgtcaccgg ttcaggagcc tgacgccaga actgcgcgcc atcccaggca atgctgccgt
    36121 tttcacccag cgtgacaatc actgttcctg cgccacgggc aacaatcgct ttcatcttca
    36181 gacgcagcgt ttcgtcttct tgcggtgcgg aggcaaaggc aaaatcgaga tgcggcacca
    36241 gtgtctgcca gagcgggctg tcccacttgt cggagaagtc gaaagcggta agtttgcccg
    36301 cagcgtgcag ctgtgggaat gcgtcttccg catgtcccca gattgccgcg tgcacaatgt
    36361 catactgcgc cagccaggcg taatcctctt cactcagggc aaagtcggcc atcacgcctt
    36421 cggtgtagtc gccaaaaacg cgatcattgt cgtgcagttc cacctgagtt tgtgcggtaa
    36481 cgccgtgttt cgtatggaca tggctgatat cgacgcccat gcgggcgaga tcctgcttca
    36541 gctttgtgcc gtagtcatcg tcacccaccc aggtaatgca tcccggctgt atgccgtagc
    36601 gagtgcagta caccgccaca ttgaccgcat taccgccaga aaacgcttta ttcagttgcg
    36661 ggtagatatc gacgcagtta tcgccgattg tcgccagggt tttcatctct catcctccgg
    36721 cagcagcgcg cgaaagcgtt ccagcgcctg gcgggcatag agtctgggct cgttcatata
    36781 catcgtcacc agctccaccg tacagtaacc ctcatagccc cgctcaataa tatcgcgcat
    36841 cagttcccgc agcggcattt tgccttctcc aggaatgtaa tgcgtgtcgc tggccccgtc
    36901 gctgtcgaca atatgcagat gacgtaattt atcgcccagt ttgtcgaaat aactcatcac
    36961 cggttccgcc tggacatacg gcgcgcaaat gtcgaccatg ctgaacaggc gcggcgaagg
    37021 caccagcgcc agcgcatgaa gcacatcatt agcattacac acgatgttcg attcatacgg
    37081 cgttaacggt tcgaggatta gatccatgcc gatgttttcc gcgtactcat acagttcgct
    37141 taggttctct gccagccgtc cccagataac attaggtggc gtgagatagc ccgcgtgggc
    37201 cgcggaaatc agcgtataac ccgcgttcat ctcttttgcc atatccatcg ccagcttgat
    37261 catgtcgagg ctttcgcgac gcatatgttc atcgcccagc atcatgttat acggatagcc
    37321 attggtttct ggcgtatagc cgataatcgg catctgatac gtctgcgcca gcgccttgat
    37381 ttgtttgatg ccgcccgctt ttaagtccgg cgcgaacgcg tgcgggcgac cgccccaaat
    37441 ttcgatgccg tcgtaaccca gctcgcttgc atcacgaaat gcgtgttcaa taggcagccg
    37501 ctggtggccg caggtaaaca tacctgtttt tatcgggtaa ctccttgcgt aaatccggcc
    37561 cgttgccggg ccgggcgagg gattaatatt ccaccagacc accgtaatag cggcgttcat
    37621 ccgggttgtg atctttgtaa atagacaggt agtagcagag ccactccatt ggcacgaaca
    37681 tcaggaacgg tgccagccac gggtgcagcc cttgcgaaat ttcggcgtaa tcgatgacga
    37741 tcacgttgtc agtacgctgt ttaacaaagt taatggcgcg ttcggtggtg tggcgacttt
    37801 catcattgcc gagcaggaac aggaacggaa cgcccggttc gacaatctcc agcgggccat
    37861 ggcggaactc tccgctctca atcacgcagc cgtgcgtcca ggtaaattcc atcagcgtta
    37921 caatgccttc tttgtaaccc agcggacgca gcggacccgc agcaacggta taaatcatcg
    37981 gccactggct ggccagttca ccaagctggc ggcctttttc ttcccaggtg cgtaccagat
    38041 gaccgagcgc attcggcaac tgcttgagat cgtttttgat cttgccgatt tccgcgttcg
    38101 gcgcgaggcg ggtgatcatc tccagcacca cgctgtagca gagcagcagg tgaatttccc
    38161 agatacagtc ggcctgataa tcaatgctaa attccgccgc cgaggtaatc gggctgtccg
    38221 cgcgtttggt gaacgccgca gtgagtgcgc cgcaggcccg gcccagctcc agcgctttga
    38281 ttacctcttc cgttttaccg tagtcagaaa cgccaatcac cgcgcaacga tcgtcgaggc
    38341 gatacggggt gttatcgcag aactcccagc cggaaatggc gtagacctga agatcggaaa
    38401 agcgatccgc cagatgtttc gccgcttgcg ccgcgttgag tggcgagccg caggcaacga
    38461 aataaatacg atcaatgtcg cgcttcacca tctcttccac gatggcgtgc accagcggaa
    38521 cgtcatggct gagaactttt tccacttcct gaaccatatt ttcggtgacc agaaagtcca
    38581 cggtgctttt atcaatatcc aacattttat tttctccaga taacattacg acaaagcgtt
    38641 gagctggcgg ctacgcttcg cccagaaggc gtaagcaggc agaccagtag caataacgat
    38701 gacggcgcag ataaggccgg gaatcggtgc ccagacaaag gttgaggcga ccagaatgag
    38761 gcttgacgca atggcgaggg tggtcatcag cccgaaagca ggagtacgcc acagcggttt
    38821 gtaatcgtcg cgtttacgac accagatgat ggagccgaag gtgagggtat ttttgaaaca
    38881 catcaccagg gtgaaataac ccagcaggct ggtgagatcg gaaacgaaga tgaagaagag
    38941 ccccagcgcc ccttgcagga tgatggagac atccggcgtg ttgtatttcg gatgcacatg
    39001 gccgaagcat ttaaagaaca ggttgtcttt cgccatcgcg tattccagcc gcggctggta
    39061 catcacgcag ctggaaagcg aaccaagaat gacgatcatc gccgtgatgg caacaaagat
    39121 cccagcggtg ctgccgagtg cggggatcca ggtcagggcg tcggaaatcg gcgtttcaga
    39181 attggcgagt ttgtcgaagg gcatcaggtc ggaaatcacc agcgccagca gggtgtagag
    39241 caccagaacc agcagacagg aaccaatcag cgctcgtggc atggtttttc cggggttttt
    39301 aatttcgccg gtcatataac agatagaggc catgccggta tacgaccaac tggtggcaga
    39361 gatccccgcc agcagcgcca taaagctgcc cgttgcgcca atcgcagtgg tggtaggggc
    39421 ggcaaaattc tccgctttaa accagaagat cccaaggcca atgacgatag tgaacgggat
    39481 aattttggcg atggtaatta gcgtctgaaa cgctgcgccg ccttcaacgg agcgcaggtg
    39541 tagcaacata aaggcgataa ttaatccggc ggcgataaat ttaccgggca acggatcgat
    39601 aggcgttaaa aagccaagat tgctgacaat cgccagcgcc ataatcgaca atgacggcgc
    39661 atcgttggcc cagaagctgg cccagccgga gaggaaagcc agcggtcggc ttccggcatt
    39721 tttcagataa acataatctg cgccattttc cggataagcg gtggatagtt ccgcatagac
    39781 gcacatttgc gggatcacaa ttaacccgcc aatgacaaac gtgagcaccg taagccacgg
    39841 cgtgcccgct gcttttgcca cttcacccac agatacaaaa ataccggagc cgacggttgt
    39901 cccgacggcg attgcaagaa cggcccaaaa tccgagcttg cgttggagtt cctggcttcc
    39961 catagcgata cctttttctt gtccaacacg ttgtagggga gagtttgcag tcatttaaaa
    40021 ggtaacatga caatgcatga tgaatataat aacatatgat gttatgtaat gcggaggaag
    40081 atcacaaaat ttcgcacagg atcgcgctgt ggctaatgaa tgtagttatc caattgaaat
    40141 taaagtgaaa atatttttac gggtggggag caagaaggat atagaaacaa atacgccctc
    40201 ggaaaatcca gagggcgtcg ggcaattaaa ccggtgttag ccgatttctg tcagagactt
    40261 actgtgcagt aggaccgcaa ccgccaatga ttttagaaat agaaattgcc gggtgcagca
    40321 ggtagtcgta agagcagttc ttatcacggt tttcaatatg accagtacat gcagttaacg
    40381 tagccagtac tgcacctacc agtgcaactt taataaattt gttcatttac
//
LOCUS       DAJTSL010000014        40081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTSL010000014 DAJTSL010000000
VERSION     DAJTSL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.014"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..859)
                     /locus_tag="OO433_000654"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="OO433_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(834..1634)
                     /gene="otsB"
                     /locus_tag="OO433_000655"
                     /pseudo
     CDS             complement(834..1634)
                     /gene="otsB"
                     /locus_tag="OO433_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
     gene            complement(1801..2787)
                     /gene="araH"
                     /locus_tag="OO433_000656"
     CDS             complement(1801..2787)
                     /gene="araH"
                     /locus_tag="OO433_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6049243.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2802..4316)
                     /gene="araG"
                     /locus_tag="OO433_000657"
     CDS             complement(2802..4316)
                     /gene="araG"
                     /locus_tag="OO433_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6049244.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGAIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(4386..5374)
                     /gene="araF"
                     /locus_tag="OO433_000658"
                     /pseudo
     CDS             complement(4386..5374)
                     /gene="araF"
                     /locus_tag="OO433_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            6088..6591
                     /locus_tag="OO433_000659"
     CDS             6088..6591
                     /locus_tag="OO433_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6049245.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6670..6921)
                     /locus_tag="OO433_000660"
     CDS             complement(6670..6921)
                     /locus_tag="OO433_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6049246.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(7036..7122)
                     /gene="azuC"
                     /locus_tag="OO433_000661"
     CDS             complement(7036..7122)
                     /gene="azuC"
                     /locus_tag="OO433_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6049247.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7385..7708
                     /locus_tag="OO433_000662"
     CDS             7385..7708
                     /locus_tag="OO433_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6049248.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7879..8376
                     /gene="ftnA"
                     /locus_tag="OO433_000663"
     CDS             7879..8376
                     /gene="ftnA"
                     /locus_tag="OO433_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6049249.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQHLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8414..8653)
                     /locus_tag="OO433_000664"
     CDS             complement(8414..8653)
                     /locus_tag="OO433_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6049250.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            8844..10055
                     /gene="tyrP"
                     /locus_tag="OO433_000665"
     CDS             8844..10055
                     /gene="tyrP"
                     /locus_tag="OO433_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6049251.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRRVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVR"
     gene            complement(10117..10782)
                     /gene="yecA"
                     /locus_tag="OO433_000666"
     CDS             complement(10117..10782)
                     /gene="yecA"
                     /locus_tag="OO433_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6049252.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDDLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10978..11064)
                     /locus_tag="OO433_000667"
     tRNA            complement(10978..11064)
                     /locus_tag="OO433_000667"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11028..11030),aa:Leu,seq:taa)
     gene            complement(11077..11150)
                     /locus_tag="OO433_000668"
     tRNA            complement(11077..11150)
                     /locus_tag="OO433_000668"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11116..11118),aa:Cys,seq:gca)
     gene            complement(11205..11280)
                     /locus_tag="OO433_000669"
     tRNA            complement(11205..11280)
                     /locus_tag="OO433_000669"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11245..11247),aa:Gly,seq:gcc)
     gene            complement(11432..11980)
                     /gene="pgsA"
                     /locus_tag="OO433_000670"
     CDS             complement(11432..11980)
                     /gene="pgsA"
                     /locus_tag="OO433_000670"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6049253.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(12037..13869)
                     /gene="uvrC"
                     /locus_tag="OO433_000671"
     CDS             complement(12037..13869)
                     /gene="uvrC"
                     /locus_tag="OO433_000671"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6049254.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEDEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13866..14522)
                     /gene="uvrY"
                     /locus_tag="OO433_000672"
     CDS             complement(13866..14522)
                     /gene="uvrY"
                     /locus_tag="OO433_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6049255.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14817..14993
                     /locus_tag="OO433_000673"
     CDS             14817..14993
                     /locus_tag="OO433_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049256.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            14980..15204
                     /locus_tag="OO433_000674"
     CDS             14980..15204
                     /locus_tag="OO433_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6049257.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(15271..15993)
                     /gene="sdiA"
                     /locus_tag="OO433_000675"
     CDS             complement(15271..15993)
                     /gene="sdiA"
                     /locus_tag="OO433_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR6049258.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(16223..16975)
                     /gene="yecC"
                     /locus_tag="OO433_000676"
     CDS             complement(16223..16975)
                     /gene="yecC"
                     /locus_tag="OO433_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6049259.1"
                     /translation="MSAIEVKNLVKKFQGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     LGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(16972..17640)
                     /gene="tcyL"
                     /locus_tag="OO433_000677"
     CDS             complement(16972..17640)
                     /gene="tcyL"
                     /locus_tag="OO433_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6049260.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPILSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17655..18641)
                     /gene="dcyD"
                     /locus_tag="OO433_000678"
     CDS             complement(17655..18641)
                     /gene="dcyD"
                     /locus_tag="OO433_000678"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6049261.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLNLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18746..19546)
                     /gene="tcyJ"
                     /locus_tag="OO433_000679"
     CDS             complement(18746..19546)
                     /gene="tcyJ"
                     /locus_tag="OO433_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6049262.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19634..20185)
                     /gene="fliZ"
                     /locus_tag="OO433_000680"
     CDS             complement(19634..20185)
                     /gene="fliZ"
                     /locus_tag="OO433_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6049263.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQAHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(20231..20950)
                     /gene="fliA"
                     /locus_tag="OO433_000681"
     CDS             complement(20231..20950)
                     /gene="fliA"
                     /locus_tag="OO433_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR6049264.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLSTKLGKL"
     gene            complement(21270..22976)
                     /locus_tag="OO433_000682"
     CDS             complement(21270..22976)
                     /locus_tag="OO433_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6049265.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRILELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGTIANKAATVSDLTAAGATGTGPYAVTTNNTALSASDALS
                     RLKTGDTVTTTGSSAAIYTYDAAKGNFTTQATVADGDVVNFANTLKPAAGTTASGVYT
                     RSTGDVKFDVDANGDVTIGGKAAYLDATGNLSTNNAGIASSAKLSDLFASGSTLATTG
                     SIQLSGTTYNFGAAATSGVTYTKTVSADTVLSTVQSAATANTAVTGATIKYNTGIQSA
                     TASFGGANTNGAGNSNDTYTDADKELTTTASYTINYNVDKDTGTVTVASNGAGATGKF
                     AATVGAQAYVNSTGKLTTETTSAGTATKDPLAALDEAISSIDKFRSSLGAIQNRLDSA
                     VTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLL
                     QG"
     gene            23242..24648
                     /gene="fliD"
                     /locus_tag="OO433_000683"
     CDS             23242..24648
                     /gene="fliD"
                     /locus_tag="OO433_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6049266.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAKNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKKQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            24673..25083
                     /gene="fliS"
                     /locus_tag="OO433_000684"
     CDS             24673..25083
                     /gene="fliS"
                     /locus_tag="OO433_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6049267.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            25083..25449
                     /gene="fliT"
                     /locus_tag="OO433_000685"
                     /pseudo
     CDS             25083..25449
                     /gene="fliT"
                     /locus_tag="OO433_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
     gene            25527..27014
                     /gene="amyA"
                     /locus_tag="OO433_000686"
     CDS             25527..27014
                     /gene="amyA"
                     /locus_tag="OO433_000686"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6049268.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYNLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDEHGEATFFCNGGSVSVWVIEEVI"
     gene            complement(27048..27461)
                     /locus_tag="OO433_000687"
     CDS             complement(27048..27461)
                     /locus_tag="OO433_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6049269.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLALMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            27648..28853
                     /gene="yedE"
                     /locus_tag="OO433_000688"
     CDS             27648..28853
                     /gene="yedE"
                     /locus_tag="OO433_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6049270.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            28850..29083
                     /gene="yedF"
                     /locus_tag="OO433_000689"
     CDS             28850..29083
                     /gene="yedF"
                     /locus_tag="OO433_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6049271.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVRDIQQDGPTIRYLIQK"
     gene            29190..29861
                     /locus_tag="OO433_000690"
     CDS             29190..29861
                     /locus_tag="OO433_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR6049272.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFEHGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(29901..31109)
                     /locus_tag="OO433_000691"
     CDS             complement(29901..31109)
                     /locus_tag="OO433_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6049273.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTAFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLLRGGQVFAVPPACTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            31117..31548
                     /gene="tnpA"
                     /locus_tag="OO433_000692"
     CDS             31117..31548
                     /gene="tnpA"
                     /locus_tag="OO433_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6049274.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQTPG"
     gene            complement(31679..33002)
                     /locus_tag="OO433_000693"
                     /pseudo
     CDS             complement(31679..33002)
                     /locus_tag="OO433_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(33002..34618)
                     /locus_tag="OO433_000694"
     CDS             complement(33002..34618)
                     /locus_tag="OO433_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6049275.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMIWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGGWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            34914..35630
                     /locus_tag="OO433_000695"
     CDS             34914..35630
                     /locus_tag="OO433_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6049276.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(35627..37288)
                     /locus_tag="OO433_000696"
     CDS             complement(35627..37288)
                     /locus_tag="OO433_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6049277.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAPALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(37484..37912)
                     /gene="ibpB"
                     /locus_tag="OO433_000697"
     CDS             complement(37484..37912)
                     /gene="ibpB"
                     /locus_tag="OO433_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6049278.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(38024..38437)
                     /gene="ibpA"
                     /locus_tag="OO433_000698"
     CDS             complement(38024..38437)
                     /gene="ibpA"
                     /locus_tag="OO433_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6049279.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(38431..38503)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            38743..39075
                     /locus_tag="OO433_000699"
     CDS             38743..39075
                     /locus_tag="OO433_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6049280.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSHVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(39077..>40081)
                     /locus_tag="OO433_000700"
     CDS             complement(39077..>40081)
                     /locus_tag="OO433_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6049281.1"
                     /translation="PKSEKYVFIHGPENPDETWYYDFHHRRGVIAEGGKVSNLDAMDI
                     TAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNVGVAAPFGPVNVQ
                     KQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAFIGDTLSPKGEKV
                     PELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTHHRAYPGLVNVPR
                     HWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQSAFNWHPSGEWL
                     GFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWMEGGQLWITETDR
                     "
BASE COUNT         9976 a        10369 c         9702 g        10034 t
ORIGIN      
        1 ttaccatgat gctgcggata tttttccagc aacgcttcat aggcgagaaa acgctctggc
       61 aaacctttgg aataatccag ccgttcgaca gaaaagatat tttgtacgtt tttcagttcc
      121 gctttaagtt gcgccagttt tggcggcagt ggcccggcag cctgtttggc tatttctttc
      181 ggttcaatgc cgatcgggta gacttctgtt cgaaatgctt tgccccaggc tgtatggctt
      241 tttgcgctac gtgtcgtgac gcgggtcagg ttagaaagac aatccaggaa cgccagacga
      301 tcgttttctg tctggaaacc cagcaaatca taatcacaaa gctgttcaag caaggtgtca
      361 tatgtcggca gcgcgttgaa gatttccggt gtcgggaaag gaatatgcag aaagaaacca
      421 atgcgattat tcaccccacg tttgcgtaat tcatgcgcaa agggcaacag gtgataatcg
      481 tggatccaga taatgtcatc gtcttgcaac agcggcagta atttatctgc cagcaacgca
      541 tttacgcgta gatagccgtc ccaggcagga cgctgaaatt gcatcagatc gagccgataa
      601 tgaaaagcgg gccagagaac ggcattggag aattggttgt agtattcgtc aaggtcctgt
      661 tcgctgaggt taaaagaggc ccacgtaatg ttaccttttt tagcggctga tcctcattcc
      721 ctgtttcacc actccagcca aaccacagtc cgcctgtggc tttcagtgcc cccagtatgc
      781 caacggcaag gccaccggca ctggcggcgt gctcgtctgg tggtgcaatc cggttagata
      841 ctacgactaa acgactcata gtcatcactc ctgttatttt ctcttttttg ttgtaatgcg
      901 gtggttatca tttcaagcct gctccagaca tccggcacac ccgccagtcg ccatgatgcc
      961 tgagttgcac ctgtgccaat ttttactgac attccgccca gtcggttaac gactgcgaag
     1021 ccagattcat cggttaaatc atcgcccaga aatacgggcg ttcgcccgat aaagggagct
     1081 tcctgcataa aagctgcaat tgcctcacct ttactggtac ctctcggttt gatctcgaca
     1141 acacactttc cctgctgtaa cgccatttgt ggccagatct gagtaatacg ttacgctaat
     1201 gtcattaatg cgtcttcatg ctgcggagcc tgacgataat gcagcgcaaa agccatccct
     1261 ttcgcctcca gctccgcgcc gggatactga gcgataactg tatgcagttg cacgctaata
     1321 tcacgcgcaa tcgcatccgg cagatgaacg atatgcgttt taccattgat gtcacggcgc
     1381 tccgccccat gcacgcccgc taacgggaag cgataaggtt ttgccagtgc gtcaagctcc
     1441 accattgagc gccctgatat caatgccaat gcaccatcac ttgcggttgc cagtagctgt
     1501 agtccttgca gaatattgtc aggcacgacg acctgatcgg gatgcggttt gatttccgcc
     1561 agcgttccat cgagatcaaa aaaccaggca tatttcgcgg atagttcagg ggtttcggtt
     1621 aacggttctg tcacccggtt ctcctacttc tcttttctta ttgttttgcg cagaacgcgc
     1681 tttacagaca tgtaagtata gacagtgtga cgggggtcgc catttgcaac gaagaacagc
     1741 caaccgttat ggcggttggc tgtggtggga aaaaacgcta aattgttgca gaaaaaagca
     1801 tcagacagtg cgtttcgctt tttgcttgta acggtcgaag atcaccgctg ccagcaggat
     1861 taagccgcga accacgtact gcgcgaaagg agaaatatta agcaggttca tggcgttttc
     1921 cacggtgcct aaaattaaga tacccgccac cacatatgag atttttccga tgcctccttt
     1981 cagagaaacg ccacctaaaa cacaggcgga gataacaatc agctcgtaac caatcgacgt
     2041 cattggctgc ccactggtca tacgtgaagc cagaataatt ccggctatcg cagataccag
     2101 gccagagaga acaaagataa taattttggt gcgaacaacc ggtacacccg ccagacgcgc
     2161 agcctcttcg ttcccgccaa tcgccagggt gttacgacca aaggtggttt tattcagcaa
     2221 caaaccaaag ataatcagac acgcgacggt gagccagatt ggcgcaggca gaccgaacca
     2281 gttagcgtaa ccaagggcaa agaagctttc atcttcgata ccgaccgctt taccgtctga
     2341 aatgatgtac gccagacctc gaacaatctg catcgttgcc agtgttgtga tcagagcatt
     2401 tattttcagt ttggcgataa caaagccatt gaccaggcca cagagaatgc caagcagcaa
     2461 ccccgctgcc acgccaatcc acaggctttc agtcaggttg ataaccaccg ccgtggtgac
     2521 acccgcacag gcaattacgg aggcgacaga aaggtcaaag tcaccggaag caaggcagaa
     2581 caacatgcca cacgccacca tccccgacat ggaaattgcc aggcccaacc ctttcatatt
     2641 aatgaaggtg gcaaaatttg ggacaaaaat ggcacaggcg ataaagagca ccgcaaacac
     2701 caccagcatg ccgtactgat cccagatacg cccgaagctg aatgacgact taggtgcgcc
     2761 agaacccgat gtagaaacag aagacatcat actctcctta ctcaggcaac tgcctggctg
     2821 actttaggca tcgcaaggct cagtgcctga cgctcatctg cctgctcgtg taacaattca
     2881 ccggcgattg caccttcccg catcaccaca atccggtcgg caacgccgag gacttcaggt
     2941 aagtcgctgg aggcaaacag caccgccaca ccctgcgccg ccagcgcata aattacgtta
     3001 tatatttcgt gcttagcacc aacatcaatg ccgcgcgtag gttcatccag caaaatgacc
     3061 ttcatctctt ccgataacca gcggcccaga atggcttttt gctgatttcc gcctgagaga
     3121 ttcatgatca gttgctcagc gcccggcgtt ttgatgttga gcgaacgaat gtgctgatcg
     3181 gcattgtttt cttcccaacc gttgttgatt acacaaccgc cgagcacatg tttgcgtctg
     3241 gcactgatgt tgatattgtc gcgaacggag tgcacgggaa taatgccttc cgctttgcga
     3301 tcttccgggc agagcatcat gcctgcggca atggcgtggc tcggtttacg aatatcgatc
     3361 ggctgttggt cgatataaac ctgaccggcg gtgatttgcg tcccgccaaa caagcctttc
     3421 attaattcgc tacgccccgc ccctaccaga ccaaacagcc caacaatttc accactgcga
     3481 accgccagac ttattggcgt acgcacgcct ggtgctttca cagcatcaag acgcaggcgc
     3541 tcctcgccat aactacgagg ttgccagccg tagatatcgc caatgtcgcg cccgaccatc
     3601 gcctgcacca gcgcgtcgtg gtcaacctgc tgcatatcgg taaaggtttt gacataacgt
     3661 ccatctttaa agacagtgat ggcatcgctg agggcaaata tttcttccat acggtgagaa
     3721 acgtataaga tcacccgccc ctcttttcgc agttcacgaa taacgcggaa aagattgtcg
     3781 atttcgcggg cagaaaggga gctggttggc tcatcaaagg cgataatttt agcgttacgc
     3841 gccagcgcct tggcgatttc aaccatctgc cactgaccaa tggagagata tttcagcggc
     3901 gtgtccgggt caatatccat accaagatgt ttaagttgta aacccgcctc ataattcagc
     3961 aatgagcgat tcacaatgcc gcctttatgc ggcagctggc cgagatagat gttttccgcg
     4021 acggtcattt ccggcacgag atgcagttcc tggtaaataa tcgccacacc cgcgttaagt
     4081 gctgcggtcg tgtcggaaaa ggacatttcc tgcccattaa tcactacaga acccgtggtt
     4141 ggcgcatagt tgccgctgag gatttttaag agagttgatt ttcctgcgcc attttcaccc
     4201 atcaacgcat gaacctgacc ggcatagcag tcaaaactaa tatccgtcag cgccttaacg
     4261 ccgggaaacg ttttaccgat gccgcgaaat gagagatacg gggtagactg ttgcataacg
     4321 tctccgtgaa tcactggtcg ttcacacccc ctctgcatca tgcagagggg aatttttccg
     4381 gcaaattact taccgcctaa accttttttc tccagttctt ctttaaagtt gtcacgcgtg
     4441 atcagcacca cgtcggtaac ttcggtaaat tttggcggtt caacgtcttt tgctacccag
     4501 ttgtaaagca tttcgctgga tttatagcca tgtacgtccg ggcttggcag cagggaaccg
     4561 tagaagccgg ttgcctgtac tttagacagt tcgctcaccg catccacacc gttaatgcca
     4621 atgccgataa tatcggcagc tttaaagccc tgaccttccg tcgcgcgtac gccgcccagc
     4681 acggtgctgt cgttcatacc gacgatcagc caatgtttaa cttcaggatg ttgaaccagc
     4741 attgagttgg cagcgtcaaa tgcccccggg atgtcgttag atttggtagg tacctgataa
     4801 atttgttttt ccgggaatcc ggccgctttc agcgcatcca tagatcccgt agtacggcgg
     4861 cgggcagtat ccagttcgtt ggcggtaatc accatcaccg cgctttcttt gacatcccag
     4921 ccacgtttct gcatctcttt atacagttcc tgaccctgac gttcgccaat tttagtcgcg
     4981 ccatcatcac cagcggaacg gtatccattg gcttaccttt ggcgttaaca aactgatcat
     5041 ccacggcaat gactttcata tcgtagccac gcgctttcgc gacgatggca gagccgagtt
     5101 tggggtccgg agtacaaata acgaaacctt ttgcgccact ggcagccagg ctgtcgatcg
     5161 cgttcaatgt tttttcgcca tccggcacgg caatcttaat aacctcaaac cctaaatcct
     5221 tcccggcttt atcggcaaac ttccattcgg tctggaacca cggctcttcc ggttgcttca
     5281 ccagaaaacc gagcttcagg ttctccgcca tagcggattg tgacataacg gctgccagac
     5341 caatggctgc cagggcttta gtaaatttgt gcatggttct ctccagcttt agtgtcgttt
     5401 tgtgtagggc aaaaacgaat gacattcgtt aaattaatcg gaaaacaaag cattaccttt
     5461 taactaaaag ataagtgacc gtgttgacat agttttagcg agaaattaat tctccatagg
     5521 agagcaatat cacatcgcag aattacagtg agaacgtgca taaatttagc gggaaaagac
     5581 ataagggaaa gccaatttgt cagacaaatt gtcgaatgca cagcagatta atccataaga
     5641 ttagcttgga aatccttgtt gtctttggta cccatgcggg atgtcttctt tttaaccagt
     5701 caataggccg cattacctgg cgttgagttt ttgaaatggt gtaataaccg caactcaaag
     5761 atgtggaaaa tgcacgtcat tcatttcgcc attaattatc actgtgctca ttaattaaca
     5821 gaacacgtat aatgagagcc atctcgcaaa aatgaaaaaa cgttttataa aatcatcatt
     5881 tcatcaatga aatgtaaaaa tatataaact tgatgattta agcattttct tatacccgtt
     5941 cagacgttat tcttatttca gatcatcgtc agaattgact ccacgatcac atttcggacc
     6001 ggcagaaagg aattattctg caaacagtaa ttatggtgtt ttgatttatc ttgcacctct
     6061 ccacttctgg atataaggat attaggtatg gcaaccgctg gaatgcttct caaactcaac
     6121 tctcaaatga accgcgagtt ttacgcatcc aatctctacc ttcacctgag taactggtgt
     6181 tctgaacaga gtctgaacgg caccgccact ttccttcgcg cccaggcaca gagtaatgtg
     6241 acccaaatga tgcgcatgtt taactttatg aagagtgtcg gcgctacccc catcgttaaa
     6301 gccattgatg ttcccggtga aaaactgaac tctctggaag aactgttcca aaaaacgatg
     6361 gaagaatacg agcaacgttc tagtacgttg gcacagttag ccgatgaagc gaaagaactg
     6421 aatgatgatt caaccgtcaa tttcctgcgc gatctggaaa aagaacagca gcatgatggt
     6481 ctgttgctgc aaaccattct tgatgaagtg cgcagtgcga aacttgctgg tatgtgccca
     6541 gtgcagaccg accaacatgt tctgaatgtc gtgtcgcatc agctgcattg atcatacatc
     6601 ggcgctaatg cattgcgccg atgaagtttt tgagaaaccg ctgcctcatc tttttgaagc
     6661 agcggttttt taatgggatt cgccttgtgg ggtaaacttg agttcaatca gcgcgatggc
     6721 tttttggatt gcccgcatgg tgaccgggtc tgcggcggcg ggatggttac taaagtcgat
     6781 attcttcagc tgactggaca ttttttcacg aacttcaacg ggcgcgatta catcgagaac
     6841 atccagaatt tgtttgataa ccaactggca agcaaccaca tcagaaacca gttcctgatc
     6901 ggcattcagc ggctgggaca tcgtaaactc ctgatagcat tttgaaaacc gttatagtag
     6961 cggcttcaca tcttcagcga tagtcacatc caccgtcatc aggacacaaa aaaacctgcc
     7021 ggagcaggtt ttttgttatc ggaacatatt gcctggcggt acgtctttga acgtcttgca
     7081 atagttattg aacatacttt tcaggatttt gcgcagtttc atcgcggcac tccgaccatt
     7141 tgttatacat gtgttatttt ctttgtgctt ataatatgac aaccatcaca aaaatcaatc
     7201 tttatgtgat gcaaatcaca caaatacccc cttaatgtta taaaaatgat aatcaaaaaa
     7261 cagccccccc atttctgaca cctacagatg gcaagaaata gcgcctgcca ggcgtctttt
     7321 ccggtcattg tcgcagcgct gtaacgcgta aaatagtgct ttcttttact cttctggctg
     7381 gaccatgaga cttctgattc tgactctttc attaataacg ctggcggggt gtacggttac
     7441 tcgccaggcc catgtgagcg aagttgacgc ggcaacaggt attgtacggt tggtttatga
     7501 ccaggctttt ttgcagcatg cccataccga tcgttatgtc agtcgcggga tcgccgatcg
     7561 cgcatgccag caggaaggct atacccacgc aatccccttt ggtcagccag taggcaactg
     7621 tagccttttt gcgggttcct tatgtttgaa tacggaattc actttgtcat atcagtgcca
     7681 tcattccgcc ttccctgtct ttctctaaat attttaatca gcgaggggat cttcgctgat
     7741 taaaaaaata gcggtaatgc gttttattcc cattcgtatt tttaataatt gaagtttata
     7801 ttttaccttt tgcaaataat aaaataacaa attatagtga cgccacagca acaaatataa
     7861 cctttgtgga gcactatcat gctgaaacca gaaatgattg aaaaacttaa tgagcagatg
     7921 aacctggaac tgtactcttc actgctttat cagcaaatga gcgcctggtg cagctatcat
     7981 accttcgaag gtgctgccgc gttcctgcgc cgtcacgccc aagaagagat gacgcatatg
     8041 cagcatctgt ttgattacct gactgatacc ggcaatttac cgcgtattaa taccgttgaa
     8101 tctccgtttg ctgaatattc ctcacttgat gaattattcc aggaaaccta taaacacgaa
     8161 caattaatca cccagaaaat taacgaactg gctcatgctg caatgaccaa tcaggactac
     8221 ccaacattta atttcctgca atggtatgtt tctgagcagc atgaagaaga gaaactgttc
     8281 aaatcgatta ttgataaatt aagcctggca ggcaaaagcg gcgaaggtct gtattttatc
     8341 gacaaagaac tctctaccct cgacacacaa aactaatact ctccggcggc aggtttactg
     8401 ccgccgtttc aaatcagtga cggcaaatct tactctgatc ggtcgaaaaa ccgtctttcg
     8461 aaggtataaa tttgcctttt gctgccagaa acgctaccag ctctcccgct gtcatccctt
     8521 ctgccgagca ggtgtgaaaa cgtgcctgtt caccaaaacg cgctttaatt gctgcttcca
     8581 gactggcatg cgtatattgc tcgcctgatt caatcatcat atttaacact tcatgaccgt
     8641 gaatagagtc catcgttcct cctcaaaaaa agcctagcgt agcgattgcc gcttatgaag
     8701 actttgcgcc agagcagaac tgaatgcttt ttattgtaca tttatattta caccatatgt
     8761 aacgtcggtt tgacgaagca gccgttatgc cttaacctgc gccgcagata tcactcataa
     8821 agatcgtcag gacagaagaa agcgtgaaaa acagaactct gggaagtgtt tttatcgtgg
     8881 cgggaaccac aattggcgca ggcatgctgg caatgccgct ggctgcggca ggtgttggtt
     8941 ttagcgtcac gttaatcttg ttgattgggc tttgggcgtt gatgtgctac acggcgctat
     9001 tactgctgga ggtgtaccag catgttccgg cagataccgg tctgggcacg ctggcaaaac
     9061 gctatctggg acgctacggt caatggctga cgggcttcag tatgatgttc ttaatgtatg
     9121 ctctgactgc ggcatacatc agcggtgccg gtgaattgtt ggcctccagc atcagcgact
     9181 ggacaggtat ttcaatgtcg gcaaccgctg gcgtgctgtt gttcactttt gttgccggtg
     9241 gcgtggtttg tgttggaaca tcgctggtcg atttatttaa ccgttttctg ttcagcgcca
     9301 aaattatttt tctggtggtg atgctggtat tgctgctacc gcatattcac aaagtgaatc
     9361 ttttaaccct gccgttgcaa caggggctgg ctctgtcagc aatcccggtg atttttacct
     9421 catttggttt tcacggtagc gtgccgagta ttgtcagcta tatggatggc aacgttcgta
     9481 agctacgcag ggtgtttata accggtagtg cgatccccct ggtggcatat attttctggc
     9541 aggtggcaac gcttggcagc attgattcaa caacctttat gggattgctg gctaatcatg
     9601 ctggattaaa cgggctgtta caggcgttac gcgaaatggt ggcctctccg catgttgagc
     9661 tggcagtgca tttatttgct gatttagccc tcgccacgtc atttctcggc gttgcgttag
     9721 gcttatttga ttatctggct gatttatttc agcgttcaaa taccgttggt ggacggttgc
     9781 aaactggtgc aattaccttt ctgccgccgt tggcgtttgc actgttttat ccacgaggat
     9841 ttgtgatggc gctgggttac gccggtgtgg cgctggcggt actggcattg attatccctt
     9901 cgctgttgac ctggcaaagc agaaagcaca atcctcaggc gggttaccgg gtcaaaggtg
     9961 gtcgtccggc gctggtggtg gtgtttctct gtggtattgc tgtgattggc gtgcaatttt
    10021 tgattgcggc agggttgtta ccagaagtga ggtgatcaga tagcctcaaa ttccttattg
    10081 ggtgccagaa ttaacgctga cacccaattt ggcctcttaa tgcaggcagc actgcttaaa
    10141 tttcttacca ctaccgcacg ggcaaggatc gttacgcccc ggtttctctt ctgctttgat
    10201 cggttgctga acagcttttt cctgcggatg cgccatccag tacgcatgta gatcaagcgc
    10261 cgccagtcga atggcatcta cgctctcttc aaacgcttct ggcgacatct tttctacccg
    10321 ctcgaagttt tcctcagtac cgtgcagcgc aatcgcctcc agcgctggtt ttaacgaatc
    10381 gggcaacgtt gaccagtcag aaagtgccac gccccgcata tagccaaagc accactcctc
    10441 aacaatcgtc agctcgctgc catcaacttc tcgcaagccg aataacggct caaactgctc
    10501 cgggaattcg ttcagacgct ctgcggtatc ggccatatgt tgaaaagcca ggttcataaa
    10561 gcgcgtcatc tctttctctg acgcccagcg cggcacatag tcagccccac cccacacggc
    10621 aaccagccac tgttccggtt caatctcttg cggagaactc aacaccgccg tcaataaatc
    10681 gtccagctcc gccacatcaa ggatggcgtg gttagtgttg tatttggtca gaatatcgtc
    10741 cagccattcc aactcacttt cgtttaacgg tcccgttttc atacgctttt ccttgtggat
    10801 ctcaactcgc cagcacctat cttacatgcc ggtccgtatc agagatactt tttgagtggc
    10861 tttgccggtg attaaaaatt aaggagggtg taacgacaag ttgcaggcac aaaaaaacca
    10921 cccgaaggtg gtttcacgac actgcttatt gctttgattt tattcttatc tttcccatgg
    10981 tacccggagc gggacttgaa cccgcacagc gcgaacgccg agggatttta aatcccttgt
    11041 gtctaccgat tccaccatcc gggctcggga agaaagtgga ggcgcgttcc ggagtcgaac
    11101 cggactagac ggatttgcaa tccgctacat aaccgctttg ttaacgcgcc aaattcttca
    11161 ggcctttcag ccagacatcc gcttgacgcc gatgtctttt aaactggagc gggaaacgag
    11221 actcgaactc gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattcccgc
    11281 attcattaag caatcagtta atcacttgat tttattatcg tctggcaatc agtgccgccg
    11341 ttcgatgcgt tgcattctac ttacctggcg cgatgagtca acgatatttt tcaccacttt
    11401 tgatcgtttg ctgaaaatta cgccgaaacg atcactgatc aagcaaatct gcacgcgcag
    11461 cgctcaaata ttgcaacatt gaccacagag tcagcaccgc agccacaaag aaaagtgcaa
    11521 taccggcgta ctcaacccaa atgttcggac gccacagcag ccaggccaac gccaccatct
    11581 gggcagtggt tttcactttc ccaatccagg agacagccac gctactgcgt ttacccaact
    11641 ccgccatcca ttcgcgtagc gcagaaataa taatttcacg ggcgatcatc gttgccgccg
    11701 gtagggtcac ccaccagctg tgataatgct cggttaccag caccatggcg atagccacga
    11761 gaactttatc tgctacaggg tcaaggaaag caccaaaccg ggtactctgg ttccagcggc
    11821 gtgccagaaa accatcgaac cagtcagtca ccgccgcgac gcagaaaatg agcgcggcgg
    11881 caaacggcga ccaggtgaca ggcagataaa agaccaatac aaagaatggg ataaggatga
    11941 cacggaacag tgtaagcaac gtagggatat taaattgcat aatgacgggt aactatctgt
    12001 tgtcagtaag attaccccta tgttgctaca gagacatcaa tgtttcaacg accagaagat
    12061 cttttctgcc agaccttgcg aaatacccgg cacttttgca atttcctcga cgctggcgtt
    12121 acgtaaacct tgcaaaccgc ccatatattt caacaacatt tgccgacgtt ttggcccgac
    12181 gccttcaatg gtttccaggg aactggtatt tttgaccttc gcccgttttt tacggtgccc
    12241 gccaatcgcg tgatcatgtg attcatcgcg aatatgctgg ataacatgca gcgcgggtga
    12301 atctggcggc aaactaaatc cctcatcttc cggctcaaag aacagcgttt ccagtccagc
    12361 cttacgatct gctcctttgg caacgccaag tagcagcgga tgatttttat cccatgagac
    12421 atccagttcg gcgaagacat ttttcgcctg cgcaagctgg cctttgccgc cgtcgataag
    12481 gatcacatcc gggatcttac tgtcgtcaat ggctttacca taacgccgac gcagcacctg
    12541 attcatcgcc gcataatcat cgcccggcgt gatgcctgta atgttatagc gccgatactc
    12601 cgcacgcagc gggccgttag catcaaacac cacacaggaa gcgacggttt gttcgcccat
    12661 ggtatggctg atgtcaaagc actccatccg cttcacttcc ggcaatttca acacgctggc
    12721 aagcgcggtc agtcgctggt gaacggtaga ttgctgcgaa agtttgctgg ttaaggccgt
    12781 cgccgcattg gtgcgcgcga gtttcagata acgcgcccta tcgccgcgag gtttggtttg
    12841 aacattaatc ttgcgtcccg ccagttctga aagggaatcg gcgagcagcg ttttatcgct
    12901 aagattaaaa tcgagcagga tctcacccgg taaggtgcgc atctggctgc cttgtaaata
    12961 gaactgacct acgaaggttt ctaccacctc gctcagttcc gtaccgccag gcactttcgg
    13021 gaaatagctg cggctgccga gcactttgcc ctgacgaatg aacaatacgt ggacacaagc
    13081 catgcccgca tcgaacgcca caccaataac gtcaaggtcg tcgccggtat tggaaacaaa
    13141 ctgtttttcg gtgacgcgtc gcaccgcctg aatttggtcg cgaatacgtg ccgcttcttc
    13201 aaactccaga ttctgactgg cagtttccat acggctaatg agttgcgtaa gcacctgatc
    13261 atctttgcca gacaaaaaca ggcgcacata ctcgacctgc tgagcgtatt cttcttcact
    13321 caccagtcct tcaacgcacg gtcccagaca gcgccctatc tgatattgca gacacggacg
    13381 cgagcgattg cgataaacac tattttcgca ctggcgaatg gggaaaatct tttgcagtag
    13441 cgccagagtt tcacgtacgg catagccatt cgggaacggg ccgaaatatt cacctttggc
    13501 atgcttcgca ccacgatgca tcgccagacg cgggtgggta tcgccactca ggaagataaa
    13561 aggataagat ttatcatcgc gtagcaaaac gttgtaacgc ggctgataga gtttgatgta
    13621 gttgtgttcc agcaacagcg cttcggtttc tgtatgagta accgttacat caatttgctg
    13681 aatctgggcg accagcgctt cggttttgcg cgaagcgagg ttgctacgga aatagctgga
    13741 aagccgtttt ttcaggtctt tcgctttgcc gacatagata accgtaccac cagcatcgta
    13801 catgcgataa acgcctggct ggctggttac ggtttttaaa aacgcttttg cgtcaaactg
    13861 atcactcact gacttgataa tgtctccgca ttacacagac catggcgaat tgccaggtga
    13921 gtcagctcaa catcgccatg aatgtttagt ttactgaaca tacgatagcg gtagctgttc
    13981 accgttttcg gactgagatt gagctgttct gagatctcat tgaccttctg gcccttggtg
    14041 atcatcagca taatctgcaa ttcacgttca gacaaactgg caaatgggct ttctgttttt
    14101 tctggttcga tctggcttaa cgccatttgt tgagcgatgt cagaagcaat gtaacgctgc
    14161 cctgaataga cagaacgaat cgcactcacg acttcctgcg gagccgcgcc tttgctgagg
    14221 tagcccgcag caccggcctg catgactttc gctggtaaag ggttttctgt atgaacggta
    14281 agcatgatga ttttgacatc agctgtggaa cgcgcgattt tacgcgtcgc ctcaagaccg
    14341 ccaatgcccg gcatactcat gtccattagc accacgtcaa cggcatttgt ccggcaccac
    14401 ttaacggcgt cttcaccgca cgatgcctca ccgacgactt ttataccctt tatatcttcc
    14461 agaatgcgtc gtatccctgc gcgcaccagt tcgtggtcat caacaagtag aacgttgatc
    14521 aaagaaatat ctccagaaat agggataacg ctactgatag ttagtcattc gtatattagc
    14581 ggtttttatt gcaactttga aacgttaaaa atggtgggta ttcgattttt ctctcgtttt
    14641 tgtcaattcg attgtccaca gttatggtca cgcccgtcac caacggctta ccggatcgtt
    14701 tcacagtaag ccctacgatg aaatgtgaca aaaattgact ttattcagca aaaatgaaaa
    14761 tcagcccgct gaatatgtaa cattaattaa cccgaacaat acgggcaaaa acattcatga
    14821 taaaaatatt tattggtcat tatattaacg tattttatag cactgccgat atcacgctca
    14881 aaaaacaacc actgctattt ttagcaaagc ttatggtata ctccgccgcc ttaacatttt
    14941 tcaccgcaaa ttttcattgc aacatgacga ggaaaataaa tgagtacgcc tgatttttct
    15001 actgccgaga ataatcaaga actggcaaat gaagtctcct gcctgaaagc gatgctgacg
    15061 ctgatgctgc aggcgatggg acaagctgac gcgggccgcg tgatgttaaa gatggaaaaa
    15121 cagcttgcgc tgatcgaaga cgaaacccag gctgcagtat tttccaaaac ggttaagcaa
    15181 attaaacaag cctaccgtca gtaatataaa accggctgag agcgtgcctt tcagccggtt
    15241 tttgcatctg gcacgcagaa agaaaagcga tcaaattaag ccagtagcgg ccgcgtaaca
    15301 ggccacctgg gtcttatttg gtgcattgat ttttttctgc atgtttttct gatggaaatt
    15361 gaccgtattc tcagagattg acaaaatcat cgctatctct gctgatgtct tcccttccgc
    15421 cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg tcatcactat
    15481 ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact gcatttttaa
    15541 ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa aggacaaaaa
    15601 gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc
    15661 gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc acattaaatg
    15721 gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat tttttgcctg
    15781 ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag gtcgcgtgaa
    15841 tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct gagcctgaag
    15901 ctctatttca tggtagacct cttctgcggc ctccatcctc tgaaaacgca acagcatcgt
    15961 gcgacgccaa ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc ctgagagtga
    16021 gtatcattta taatgataat tcaaactatc acataaataa gatttatata taatttatat
    16081 tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat taatgagtta
    16141 tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg acaggtgaat
    16201 cgtcggggct gattttttct tattattgca gcagaaactt ctcgaggaac tgtcgggtgc
    16261 gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact atccgcccca
    16321 ggtccataaa aatcgcccgg tccgcaacat cccgggcaaa gctcatttcg tgcgtcacaa
    16381 tcaccatcgt gcgcttttcc tgcgccagct ggcggattgt gttcaggact tcgcccacca
    16441 gctctggatc cagcgctgac gttggctcgt caaacaaaat cacctcagga cgcattgcca
    16501 gcgcacgcgc aatcgctaca cgctgctgtt gaccgccaga caaacgacgt ggatagctgg
    16561 tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc gtggcctctt
    16621 ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc accgtacgat
    16681 gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac tggcgaatca
    16741 gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca acggtgatcg
    16801 ttcccgcttc cggttgttcc agcagattta tgctgcgtag caacgtggtt ttgccggaac
    16861 cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg ataccgtgca
    16921 gcaccgtctg accttggaat tttttcacca ggttcttaac ttcaatggca ctcatttggg
    16981 ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg ataacacagt
    17041 cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct ccagcgtgcg
    17101 cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg cggccagcga
    17161 cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca cccgcgccgc
    17221 ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac tggcagcggc
    17281 ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg cggcataggc
    17341 ggcagtgttc agcgacagac caatcattgc tgacagaatg ggatctaatt cgataccaaa
    17401 ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac cacgaaaaat
    17461 agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc gcatcagcgc
    17521 gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt gcagggtata
    17581 cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac tttcttgcat
    17641 gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc gccgccggta
    17701 tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc gtcaatcagc
    17761 cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag acgcgccagc
    17821 aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc aaaatagtca
    17881 tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat cgcctgttgt
    17941 aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac gccaatcagt
    18001 tcgctttcag gcatcaggtg ttccagccca acagccaatc cggcgtgagt tccggcactg
    18061 cccgatgcga ccaccaccga cgaaatatta accgcccctt cacactgttg cgcgatttcc
    18121 agcgcactct ccacataacc cagcgcgccc agagcattag aaccgccaac cggaatgaca
    18181 tacgggcgaa agccttgtgc ttcgactcgc gtcgccagct cttccagttg ggcattggga
    18241 tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gattcagcaa caaacgattg
    18301 ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag cgccacgcag
    18361 tgcaggccga gtttcgccgc aactgcggca gtctgccgca catggttaga ctggatcgcg
    18421 ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag aaattccagc
    18481 ttacgtaatt tattgccgcc cattgccatg ggtgtgacgt catcccgttt gatgaaaatt
    18541 tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg cgcgccgata
    18601 aactccagcc gtggaaaacg ggttaaatta tgcagtggca taatagcctc cgatgtgtgt
    18661 tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc gctagtgaaa
    18721 gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac catttttcgg
    18781 aaagggcttg cagagtgcca tctttttgca tttccgcaat tgcatcgttc actgctttca
    18841 gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag aatgcttcac
    18901 cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc agacggtcaa
    18961 cgaggatcgc atcgatacgg cctacgcgca gatcctgata tttggtcggg tcatcatcat
    19021 aggtacgcac atcgacgccc tgaacattct gccgcagcca ctcttcatag ttggtgccca
    19081 gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg ccttcgttac
    19141 cttttttcac cagcgcctga ataccagaaa tggtgtacgg ggtagagaaa tcgtattttt
    19201 tcttgcgctc atcagaaatg gtgacctgat taatcaccac atcaatacgt ttagagtcca
    19261 gcgacgctag cataccgtcc catttggtcg gttttagtga cgcctcaacg ccaagatgtt
    19321 ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca tccccctgaa
    19381 aactgaacgg cggataagtt ccttccagcc ctaccagtag cgtaccgcgc tctttgactt
    19441 tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc agcgccacgg
    19501 ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc accccgaatg
    19561 ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca actccgctac
    19621 atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc tgcacaagtc
    19681 cggtacacgt ttgacgctga tagtgttgat acttccgtaa tgcgatgcgg taattgttgg
    19741 tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc tgcagctgat
    19801 cgagggaaat attttgctca tgtagatgat tccccaaacg gcgcaaacga acgacatatt
    19861 cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaattgctta aagccgataa
    19921 tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg cacaatgccg
    19981 cccatgactg ttgttccact tgccagccat tttcatcaag cagcgtgtcc aggcgggaaa
    20041 tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg agtaatttac
    20101 ggcaatgcgc gcaatgggcc tggctgtgtt taaagtcttt aagatagcgg cttaatggcc
    20161 gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta aagtgcggca
    20221 tttactgacg ttataactta cccagtttag tgcttaaccg tttaatggcc tggctgtgta
    20281 actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg agattcagct
    20341 cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt tcgatagctt
    20401 ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt tctcgctgat
    20461 gatcatcagt aaccagttcg atgctatcgc cgtgctcttc gcgccactca tcgtaggaga
    20521 aaagctggct gttattggtg tcgagcaaca tttggcgata atcggcaata tcgatcccta
    20581 aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc agttgcccta
    20641 ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag tcacggctgc
    20701 gaagttcatc cagcatagcg ccacggatac gctgcactgc gtaagttgta aatgccgttc
    20761 cttgtagggc gtcatagcgt tcgacggcat taagtaaccc aatgccgccc gcctgtagca
    20821 gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct tcgtgacgca
    20881 ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca gcggtataga
    20941 gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta tccgtttctg
    21001 cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg gttaccggta
    21061 aattccatgc agaaaaaacc ccgccggtgg cggggaagca cgttgctgac aaattgcgct
    21121 ttatgttgcc ggatgcggcg taaacgcctt atccggccta caaaaatgtg caaattcaat
    21181 aaattgcaat tcaccttgta ggcctgataa gtgcagcgca tcaggcaatt tggcgttgcc
    21241 gtcagtctca gttaatcagg ttacgacgat taaccctgca gcagagacag aacctgctgc
    21301 ggtacctggt tggcttttgc cagcacggag ttaccggcct gctggatgat ctgcgctttc
    21361 gacatgttgg acacttcggt cgcatagtcg gcgtcctgaa tacgggactg cgcttcagac
    21421 aggttggtag tggtgttgtt caggttggtg actgcagaat ccagacggtt ctgaatagca
    21481 cccagggatg aacggaattt gtcgatggag ctgatagctt catccagggc agccagagga
    21541 tctttggttg cagtgcctgc actggtggtt tcagtggtca gtttgcctgt agagttaaca
    21601 taagcctgtg ccccaacagt agctgcaaat ttaccagttg cacctgcgcc atttgaagct
    21661 acagttactg taccggtatc cttatcgacg ttgtagttga tagtgtaaga tgcggttgtg
    21721 gtgagctctt tgtctgcatc agtataggtg tcattcgaat taccagcacc attagtattc
    21781 gcaccaccga aggacgccgt tgcagactga atacctgtat tatacttaat tgtcgcacca
    21841 gtaactgctg tgttagccgt tgcagcactc tgcactgtgc tcagtacagt atcagcgctt
    21901 acagttttgg tgtaggttac gccagaagtt gccgctgcac caaagttata agttgtgcca
    21961 gacaactgga tagaaccagt tgtcgctaag gtactaccgc tagcaaacag atcggacaat
    22021 ttcgctgaag atgcaatgcc ggcgttgttt gtagataggt taccagtggc gtccaggtac
    22081 gcggctttac caccgatggt cacatcgcca ttagcatcta catcaaactt cacatcacca
    22141 gtactacgag tataaacacc tgatgcagta gtgccagccg ctggtttcag agtattcgca
    22201 aagttaacaa catcgccatc tgcaactgtt gcttgagtgg tgaagttccc tttagccgca
    22261 tcataagtat agatcgcagc actcgagcca gtagtagtaa ctgtatctcc ggttttcagg
    22321 cgagacagtg catcgctagc gctgagtgct gtattgtttg tggtcacagc ataaggacct
    22381 gttcccgttg caccagcagc ggtcagatcg ctgactgtag cagctttgtt cgcaatagtg
    22441 cctttgccat tcacgttaaa accattcagc cccagcgtat ctgagtcaat tttcttcaga
    22501 tcaatcgtga tagtctggcc gtcattcgca ccaacctgaa ttttcattga accgtctttc
    22561 gccagtacgt tcacgccgtt gaactgggtc tggccagata cgcggtcaat ttcgtccaga
    22621 cgggatttga tttcgtcctg aatggagtcc agatccgaat cggagttagt cccggtagaa
    22681 gcctgaaccg tcagttcaag aatacgctgt aagttgttgt tgatttcgga cagcgcgcct
    22741 tcagtggtct gcgcaacgga gataccgtcg ttggcgttac gggccgcctg agtcaggcct
    22801 ttaatattag aagtaaaacg gttagcaatc gcctgacctg cggcgtcatc cttcgcgctg
    22861 ttaatacgca agccagaaga cagacgctcg atagaactcg acagcgcaga ctggttcttg
    22921 ttgatattat tttgagtgat cagcgagagg ctgttggtat taatgacttg tgccatgatt
    22981 cgttatccta tattgcaagt cgttgattac gtattgggtt tccacccgtc ggctcaatcg
    23041 ccgtcaaccc tgttatcgtc tgtcgtaaaa caacctttag aatttttttc acaaacagcc
    23101 attttttgtt agtcgccgaa atactctttt ctctgcccct tattcccgct attaaaaaaa
    23161 acaattaaac gtaaactttg cgcaattcag gccaataacc ccggtattcg ttttacgtgt
    23221 cgaaagataa aaggaaatcg catggcaagt atttcatcgc tgggagtcgg gtcaggtctg
    23281 gatttaagtt caatccttga tagcctcacc gccgcgcaaa aagcgacgct aacccctatt
    23341 tcaaatcagc aatcgtcgtt taccgctaaa cttagcgcct acggtacgct gaaaagcgcg
    23401 ctgacgactt tccagaccgc caatactgca ttgtctaaag ccgatctttt ttccgccacc
    23461 agcactacca gcagcaccac cgcgttcagt gccaccactg cgggtaacgc catcgccggg
    23521 aaatacacca tcagcgtcac ccatctggcg caggcgcaaa ccctgaccac gcgcaccacc
    23581 agagatgata cgaaaacggc gatcgccacc agcgacagca aactcaccat tcaacaaggc
    23641 ggcgacaaag atccgattac cattgatatc agcgcggcta actcatcgtt aagcgggatc
    23701 cgtgatgcta tcaacaacgc aaaagcgggc gtaagtgcga gcatcattaa cgtgggtaac
    23761 ggtgaatatc gtctgtcagt cacatcaaat gacaccggcc ttgataatgc gatgacactc
    23821 tcggtcagcg gtgatgatgc gctacaaagt tttatgggct atgacgccag tgccagcagc
    23881 aacggtatgg aggtctcggt cgccgccaag aatgcgcagc tgacggtcaa caacgtcgcc
    23941 attgagaaca gcagcaacac catcagcgac gcgctggaaa acatcaccct gaacctgaac
    24001 gatgtcacca cgggcaacca gacgctaacc atcactcagg acacctccaa agcgcaaacg
    24061 gcgattaaag actgggtgaa tgcctataac tcgttaatag ataccttcag tagcctgacc
    24121 aaatacaccg ccgtagatgc gggagctgat agccagagtt ctagcaatgg cgcactgctc
    24181 ggcgactcca cgctgcggac gattcagacg cagttgaagt cgatgctgag taataccgtc
    24241 agttcttcca gctataaaac gctggcgcag attggtatca cgaccgatcc cagcgatggc
    24301 aaactggaac tggatgccga caaactcacc gctgcactga aaaaagatgc cagcggcgta
    24361 ggtgcattga ttgttggcga tggtaaaaaa accggcatca cgaccaccat cggcagcaac
    24421 ctgaccagtt ggctttcgac aacgggcatt attaaagccg ctaccgatgg cgttagtaag
    24481 accctgaata agttaactaa agactacaac gccgccagcg atcgcattga tgcgcaggtc
    24541 gcgcgctaca aaaaacaatt tacccaactg gacgttttaa tgacctcgtt aaacagcacc
    24601 agcagctact taacgcagca gttcgaaaac aacagtaatt ccaagtaatc attattcatc
    24661 gggagacagg tcatgtacgc ggcaaaaggc acccaggcct atgcacaaat tggcgtcgaa
    24721 agcgccgtaa tgagcgccag ccagcagcag ctggtcacca tgctatttga tggagtgctg
    24781 agcgcactgg ttagagcgcg cctgtttatg caggacaaca atcagcaagg caaaggcgtc
    24841 tctttgtcaa aagcgatcaa catcattgag aacggactgc gggtgagtct tgatgaagag
    24901 agcaaagacg aactaaccca aaacttgatt gctctttata gctatatggt caggcgcttg
    24961 ctgcaagcca atttacgcaa cgatgtctcc gcagtcgaag aagtggaagc attaatgcgc
    25021 aatattgccg atgcctggaa agagtcgtta ctctcccctt ctttgattca ggacccagtc
    25081 tgatgaacca tgcaccgcat ttgtatttcg cctggcaaca actcgtcgaa aaaagccagc
    25141 tcatgttacg cctggcaacg gaagaacaat gggacgaact catcgccagc gaaatggcgt
    25201 atgtgaatgc ggtgcaggag tatagcacac ctgactgaag aggttgcccc gtccaccacg
    25261 atgcaggagc agctccgccc gatgctgcgc ctgattctcg acaacgaaag caaggtaaag
    25321 cagttattac agattcgtat ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa
    25381 aaatcggtgt taagtgccta tggcgatcag ggcggctttg tgctggctcc gcaagataac
    25441 ttctcgtgaa tcttaacgag ctaatggttc gcgaataatc cgattacggc tacgcttcta
    25501 atgttcccct tgaatggagt cgaagaatgc gtaatcccac gctgttacaa tgttttcact
    25561 ggtattaccc ggaaggcggt aagctctggc ctgaactggc cgagcgcgcc gacggtttta
    25621 atgatattgg tatcaatatg gtctggttgc cgcccgccta taaaggcgca tcgggcgggt
    25681 attcggtcgg ctacgactcc tataatttat ttgatttagg cgagtttgat cagaaaggca
    25741 gcatccctac taaatatggc gataaagtac aattgctggc cgccattgac gccctgaaac
    25801 gtaatgacat tgcggtgctg ttggacgtgg tagtcaacca caaaatgggc gcggatgaaa
    25861 aagaagctat tcgcgtgcag cgtgtaaatg ctgacgaccg tacgcaaatt gatgaagaaa
    25921 tcattgagtg cgaaggctgg acgcgttaca ccttccccgc ccgtgccggg caatactcac
    25981 agtttatctg ggatttcaaa tgttttagcg gtatcgacca tatcgaaaac cctgatgaag
    26041 atggcatatt taaaattgtt aacgactaca ccggcgaagg ctggaacgat caggttgatg
    26101 atgaattagg taatttcgat tatctgatgg gcgagaatat cgattttcgc aatcatgccg
    26161 tgacggaaga gattaaatac tgggcgcgct gggtgatgga acaaacgcaa tgcgacggtt
    26221 ttcgtcttga tgcggtcaaa catattccgg cgtggtttta taaagagtgg atcgaacacg
    26281 tacaggaagt tgcgccaaag ccgctgttta ttgtggcgga gtactggtcg catgaagtcg
    26341 ataaactgca aacgtatatt gatcaggtgg aaggcaaaac catgctgttc gatgcgccgc
    26401 tgcagatgaa attccatgaa gcatcgcgca tggggcgcga ctacgacatg acgcagattt
    26461 tcacgggtac attagtggaa gccgatcctt tccacgccgt gacgctcgtt gccaatcacg
    26521 acacccaacc gttgcaagcc ctcgaagcgc cggtcgaacc gtggtttaaa ccgctggcgt
    26581 atgccttaat tttgttgcgg gaaaatggcg ttccttcggt attctatccg gacctctacg
    26641 gtgcgcatta cgaagatgtc ggtggtgacg ggcaaaccta tccgatagat atgccaataa
    26701 tcgaacagct tgatgagtta attctcgccc gtcagcgttt cgcccacggt gtacagacgt
    26761 tatttttcga ccatccgaac tgcattgcct ttagccgcag tggcaccgac gaatatcccg
    26821 gctgcgtggt ggtcatgtcg aacggggatg atggcgaaaa aaccattcat ctgggagaga
    26881 attacggcaa taaaacctgg cgtgattttc tggggaaccg gcaagagagc gtaataaccg
    26941 acgaacacgg cgaagcaacc ttcttttgca acggcggcag cgtcagcgtg tgggttatcg
    27001 aagaggtgat ttaaattcat ccccggcggc aagccgggga gatttcatta cggcagtggc
    27061 gtcggtaacg gcgctttatc cagcgcggcg gcacactctg ccgtcgggcg atccacacgc
    27121 tccatcgtca taccatcgta ctcaatggtg ttaccgtcac gctcaacctc gtacagctca
    27181 cgttttacgg taacgttagt gagatcatca gacatcaatg ccaacttacc cggcacagca
    27241 atcacccgct gccactgacg gcaatccaga gtatcaccct ctttagtgac aatcaggctg
    27301 cctatcgctt ccggactgac cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc
    27361 agccagtccg gcgcgggggt tttcacgaca ttgttatagt tttcgacttc ggcacagccc
    27421 gccagcaaca tgagcgcgcc tgcaattgcg agttttttca tcattcgtcc tgcatgagaa
    27481 gaaaagagga ttgtggcatt aaagccctga tgtcgccagc ctttccaggt tcagagagaa
    27541 atttgatcta actattattt ttgtgaatta ttaccccgaa aataaatttg catgatgtat
    27601 gattcgcgac tcttgtttca aaccttctga gttcagaggc tacattcatg tcatggcagc
    27661 aattcaaaca cgcctggttg attaaattct gggcacccat ccctgcggtc atcgcggcgg
    27721 gtattctctc cacttactat tttggcatta ctggcacctt ttgggctgtc acgggtgaat
    27781 ttacccgttg gggcggccag ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt
    27841 attttaaaat tatccatctg gaaggatcgc cattaacccg catcgacggg atgatgatcc
    27901 tcggcatgtt tggcggctgc tttgccgcag cgctgtgggc caacaatgtc aaactgcgca
    27961 tgccacgcag ccgtatccgc attatgcagg ccattattgg cggtattatc gctggttttg
    28021 gcgcgcgtct ggcaatgggc tgtaacctgg cggcgttctt taccggtatt cctcagttct
    28081 cgctgcatgc ctggttcttt gccatcgcca ctgccattgg ttcatggttt ggcgcacgct
    28141 ttaccctgct gcccatcttc cgtattcccg tgaaaatgca gaaagtttct gccgcctcac
    28201 cgctgacgca aaaaccggat caggcgcggc gtcgttttcg tctcgggatg ctggtctttt
    28261 tcggtatgct gggctgggcg ctgctcacgg cgatgaacca accaaaactg gggctggcaa
    28321 tgctgtttgg cgtcggcttt ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg
    28381 cgttccgcga tatgtggatc accggacgta cccatatggc gaaagcaatc attatcggta
    28441 tggcggtgag tgccatcggg atcttcagtt acgtacagtt aggcgttgaa cccaaaatca
    28501 tgtgggcggg accaaacgcg gtaattggtg gtttactgtt tggttttggc atcgtgctgg
    28561 caggcggctg cgaaaccggc tggatgtacc gcgcggtaga aggccaggtg cactactggt
    28621 gggtcggtct gggcaatgtg atcggctcga cgattctggc gtattactgg gatgatttcg
    28681 ctccggcgct ggccaccgac tgggacaaaa tcaacctgct gaaaaccttt ggcccgatgg
    28741 gtggcctgct ggtgacatat ttgctgttgt ttactgcgct gatgttgatt atcggctggg
    28801 aaaaacgctt cttccgccgt gcggcaccgc agactgctaa ggagatcgca tgaaaaatat
    28861 cgttcctgat taccgccttg atatggtggg tgaaccctgc ccttatccgg cagtcgcaac
    28921 ccttgaggcg atgccgcagt taaaaaaagg ggaaatcctg gaagtggtga gcgactgtcc
    28981 acagtcgatc aataatattc cactggatgc gcgtaatcac ggctatacgg tgcgggatat
    29041 tcagcaagac gggccgacca ttcgttattt aattcagaag taatccctca ttcccgctgt
    29101 ggcccacagc gggaacatcg ttgcctctga cacatagatt ttacccgccc cctctcctat
    29161 tcttttcagt atgatctcag gaggtttcta tgtgtggacg ctttgcccaa tcccaaacgc
    29221 gtgaagatta ccttgcgctt ctcgcggaag atattgaacg cgatattccc tacgatcccg
    29281 aacccattgg cagatacaac gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg
    29341 atgaacacct tcatctggat ccggttttct ggggatatgc tcccggatgg tgggataaac
    29401 cgccgctgat taacgcccgc gtagaaactg ccgcaaccag tcgcatgttt aaaccgctct
    29461 ggcaacatgg tcgggcaatc tgttttgccg atggctggtt tgagtggaaa aaagaaggcg
    29521 acaaaaaaca gccttttttt atctatcgtg ctgatggaca acctattttt atggccgcga
    29581 taggtagcac accatttgag catggtgacg aagccgaagg atttttaatt gtcactgctg
    29641 cagcagacca gggtctggta gacattcatg accgccgccc gctggttctg tcgccagaag
    29701 ctgcacagga atggatgcgg caagagattg gcggtaaaga agcatcagaa attgctacca
    29761 acggctgtgt gccagcaaac cagttcacct ggcacccggt atcgcgcgcg gtaggtaatg
    29821 tcaaaaacca gggcgcggag ttaattcaac ctgtttgttg acatcctcca cgccctgaag
    29881 gacgaggctc cctgctacgt tcaggctgtc gcctgaatca tttcggtggg ttcctgcttc
    29941 aacgggcggc ctgactgcac catctctcca caggcaagaa cggcgtgccc cgccgctaaa
    30001 atgttacgag cgccatttac atcggcgttc gctgtatatc cacatacctg gcatctgaat
    30061 ttactttgtg acaggcggtt ttctttcgct gtatgaccac agcacgcgca acgctggctt
    30121 gtgcacgctg gcggaacagc aaacacctga ccgccacgca agagctgctt gtactcaagc
    30181 tggcggcgca tttcatacca gccctgatcc agtatcgaac ggtttaaacc tgattttgcc
    30241 cggacattgc gccccggctg gctgaccgta cccgctgctg actttgacat gttgctgacc
    30301 ttcaaatcct caatgactat cattgcgtgg tttttgctga cggtcgttgt gactttgtga
    30361 aggtagtccc tgcggatatt tgcgatacag gaatgcagtc gctgtatttt gcgtttctgc
    30421 ttctgccagt tgttgctgaa tttgaccttg cggcttaact gtcgctgaag tctcgccagc
    30481 ttcttctggt ttttctgaaa actgtttaca ggctcaaagg ctgtgccatc tgacagcgtg
    30541 gcgagtttag ccacgccagc atccagcccg accattgatg ctgaagggtg aaccggagtt
    30601 gatacttcac tttctgtctg aatactgatg taccacttac cgcaggactg gctgacagtg
    30661 acatttttca caacacccgt gacctgacgg ctgttccggt agcgcatcca gcccagtttc
    30721 ggcagaaaaa tacggctgtt ttcctgatcg agcttaacgc cctgcgggta gcggaataca
    30781 tcattctgtc cccgcttttt gaatcgggga aaagcagccc gattctggaa gaagtttttg
    30841 taagcccgct caaggtcttt cagtgactgt tgcaatggct gtgacggggc atctttaagc
    30901 cattgcgttt cagtggcatt tttccactca accagccagg aagccatttt cccgtaaggg
    30961 atgtatttat taccgacctc atgattctca ttctgacgtg ccagagcacg attgaaaacg
    31021 aaacgacatg cgcctgcgaa gcgcctcatt tcacgctcct gttgaccacc gggtcttaac
    31081 tggaatttaa atgcttgtag tcgtttcaca ggccaaatga tactttggtc tgtgaaaaaa
    31141 gaaaccgata tccggcgtgg aaggcattgt gttttcctga tgcatgttca cctggtcttt
    31201 gtcaccaaat accgacgtca gatttttgac catgacgcga cagaaaaact acgcacttac
    31261 ttttcaaatg tatgtgctga ttttgaagct gaactggttg aaatggatgg cgaaccagat
    31321 cacgtccatt tgttaattaa ctatcctccc aaactggcga tatccagtct ggtaaacagc
    31381 ctcaaaggcg tatcgggtag gttactgcga cgagatcgac cagatattgc agtcaggtat
    31441 tactacaaag gcgttttgtg gagtcctggc tattttgcca atagctgcgg aggtgcgcca
    31501 atatccgtca tccgccaata cattgaacaa cagcaaacac ctggttaggt ggaaaaccgc
    31561 gccttatatc cccgccctga aggacggggt tttacggcgc accggataaa tttggttatg
    31621 cgccgtcagc cgtgctaaag aatacggacc agcacccaac ggatgccagt ccacacaatt
    31681 aacgtaactc tggccagtac gctttgttgg cctccagcaa ctcatccaga atagctttgg
    31741 cgaccgaggc gctcggtacc gttttcgata acgtaatcgc ctgccacagg tgctgatatg
    31801 aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc cacttgctga ctcattaacc
    31861 ctttctgaaa ctgcgggata tcgccgacca ccagtggttc tggtccgttg tgcccgacca
    31921 gacacgggat ctcgaccatc gcttcatcat caaagttatg gattgccccg ttgttaggca
    31981 caatcagcaa catccgctcc tgagtgttga aggcaattgc cgccgccaga tcgacgatgt
    32041 atgacgcatg ttcgtcaatc tccagcttgg ccgccgctga atttcctgcc gctgtaatgg
    32101 cgcggcaagc atcgaaaacc tgtttctcgc gatgttccat tcacctcatt cgcgcgggta
    32161 tgttcagggt tggaatgctg aacaacataa tccgggaaga gataatattt cagataggtg
    32221 ttcggcaatg tatccgggtc cagtgcctgg acatcccgcg cttttgcata ggtgtcgttc
    32281 cagctcgctt cagtatgtgt atcttgctgc ggtggaacat aaccgtgctt agagacatat
    32341 tgccgcagct ggggcatcag gtcgttgccc tgcaaatctt caattgatgt ccaccagcca
    32401 aagtggttca ggccgtagta gcgcacgcgc atctgtttgc gatcttgcag cccaacaatt
    32461 tgcgccatcc ggctttcaat accgattggc atgtcacaga tgttgaggat tttcgcattc
    32521 gggcgcagac gacgcgtggc ttctgcgaca attgctgccg ggttggagta gttgagcatc
    32581 caggcatttg gtgaatattt ttccatataa tccaccagtt ccaggacgcc gccaatggaa
    32641 cgcatgccgt acgctattcc gccgggtccg caagtttcct gaccaacaac gccgtggcgc
    32701 agcgggattt tttcatccag ttcgcgcatc gggtatttgc cgacgcggat gtgcgccatg
    32761 acaaaatcaa cgtcgctgaa tgccacttca ggatcggtgg tgtaactaaa cgcaatgtcc
    32821 ggcgctttct ctttaaggat gactttacag gcttcggcaa tcacttcctg ccgcgcacca
    32881 tcgttatcat aaaatttcag tgcacgaagc gggaaacggt cctgattcgc caggagcatc
    32941 aacacgatgc ctggcgtaaa ggtgcttcca ccgcctgcga caaccactga gaatttggtc
    33001 attatactgc ctccgtaatg gcaacatttt ctgctgattg atgagaatta attaagctat
    33061 cgagctgttc acgcagctgg gatacatgca gaccaatgat cacctgaatg gcatcgccac
    33121 tacggaagac gccgtgcgct cccagctttt taaagacttc gtcatccagc gtttgtgaca
    33181 tgtcatgcag tgcaatacgt aaacgcgtcg cgcaattgtt aatgctggag atattgccga
    33241 ccccgcccag ggcttgcagg ataccggcag cctgatccag ctcttttttt ggctctgcag
    33301 cggtggtttg gcctcgcgag gctttgtatt cggcttttga gtagagtttc acttccgcat
    33361 cttcacgtcc cggcgtgcac atgttgaatt gcagaatcag tgtgcggaaa accacgaagt
    33421 acagcagggt aaagcacaac ccaatggcga tttgggtcag catcatatcc gcgtggttgc
    33481 tgaacatcgg gatccagttt tgcggtaaaa cctggtcaat cagacctccg cccatgttgc
    33541 ccaccacacc aaagagatac atcacggtcg acattgaggc cgccagcacg gcgtgtaccg
    33601 caaacagcaa cggtgaaatg aacaggaagg taaattccag cggttcggta attcccacca
    33661 gcatggcggt taaggttgcg ggaatcagca agcccgccac ttttacccga ttttccggtg
    33721 cggcagtgaa gtacatcgct aaagaaatgc ccacggcacc aaagattttt gagttaccgt
    33781 gcagggcaaa accgccttcc gggaacaacg atttcagcgg ctcggcgctc agactgaact
    33841 cttgcagatg ctgcgcccag tacatctgaa tgccgccttc aacagctgcc ggaccaaaga
    33901 taaactgtcc gtagatgaag tggtgtaaac cggttgggat cagaatacgt tcgaggaagg
    33961 tgtaaaccca ccccccaagc gcacccgccg aacgcaggaa cgcttgcaga gattcaatcc
    34021 ccatttgtac ttttggccaa ccgagcaacg tcagccaggc acaggggatc atcaccagga
    34081 aggcgataat caccacataa gacgtcccct ggaaaatgcc gagaaaaacc ggcagttttt
    34141 tatcgaacag acggttatgc agcgccgtca caatgccgga aatgataatt gcgccgataa
    34201 tgctggtatc gagggtttta atcccggcca tcattgtcag accgctacct gccaccgcgt
    34261 cctgagtgaa atcgacgccg aagtagcttc cccagatcat tcccatcgcg ttgatgaaat
    34321 agttccaggt caggaaactc accatcaccg ccagacaagc acgcccctgc gcttgcttag
    34381 caaggccaat gggtaaaccg acagcaaaaa tcagcggcat attacggaat accgtccaac
    34441 cgccctcttc aataatgtgt acgatttgcg cgaataaact gttcggatcg gtcagtgatt
    34501 ccccgacaaa catcgggttt tgcagcaaga tggcaagacc caccacaatc ccggcgaagg
    34561 gaaacagcag cactggcgtg aacatcgcgc cgccaaagcg ttgaatttga ctgagcattt
    34621 ccacattctc ttttctcaat tctgtaagta ggaagcctga ttggttatgc ggcaggcctg
    34681 gagcgaggat aggaagatgc gatcggcaga acatgacgac ccaatgggat tcgtgatcgc
    34741 tttcatgctt ttcgttgggc ggtagagatc tacgttttcg cgaaaaagta gacatctgtg
    34801 agcactggta cacagcatcg aacacatctt taaaaaaaag atgttttttc aatcgattaa
    34861 gcagaacttg tgggcgcatt acccgggctt gcaggcaaaa aagagatcta gagatgatct
    34921 acaaaagcat tgcggagcgg ttaagaattc gacttaattc cgcggatttc acgctaaaca
    34981 gccttcttcc cggtgaaaaa aagttggcgg aagaatttgc agtatcccgg atgaccatcc
    35041 gtaaagcgat tgacctgctg gtagcgtggg ggctggtggt ccgccgccac ggcagcggca
    35101 cttacctggt gcgcaaagat gtgctgcatc aaaccgccag cctgaccgga ctggtggagg
    35161 tgttaaaacg gcagggaaaa acggtcacca gccaggtgct gatttttgaa atcatgcctg
    35221 cgcctccggc cattgccagc cagttacgga ttcaaatcaa cgagcagatc tacttctccc
    35281 gtcgcgttcg ttttgtggaa gggaaaccgc tgatgctgga agacagctat atgccggtta
    35341 aactgttccg taatctttcg ctgcaacatc tggaagggtc gaagtttgaa tatattgaac
    35401 aagagtgtgg gattttgatt ggcggtaatt atgaaagcct gacgccggtg ctcgccgata
    35461 gactgctggc gcggcaaatg aaggtagcgg aacacacgcc actgctgcgg atcacctcgt
    35521 tgtcatatag cgagagcggg gagtttttga attattcagt gatgttcaga aatgccagcg
    35581 aataccaggt ggagtaccat ttacggcgac tccacccgga aaagagttaa ccgatactcc
    35641 agaagagcac cgccagtaat tggggggtga taattcgcag aaacatcact aacggataga
    35701 cagtggcgta agagagcgcc ggcgcaccac tggttggatg aagattatta gcaaacgcca
    35761 gcgccggagg atcggtcatg gaacctgcca gcatcccgca catggtcagg taattcattt
    35821 tggctaacat ccgcgccaga atgccaacag taatcagcgg aacggcggtg atcagggcac
    35881 cataaccaat ccagcttagc ccttcgccat tgaccagggt attcacaaaa tccccaccag
    35941 atttcagacc aacgaccgag aggaacagca cgatccccag ctcccgcagc gcgaggttgg
    36001 cacttggcgg cataaaccag tacagcttgc cgatactgcc gatacgcccg aggatcaacg
    36061 ccataatcag cggaccgccc gccagcccca gtttcaacgc ggccgggaac ccaggcacaa
    36121 agacgggaat agaacctaac aatacgccaa gcccgatgcc aataaacact ggcagcatct
    36181 gaacctgttg cagtttttgc tgcgcattcc ccagcacatt ggcaacggca tcaattgcgg
    36241 acggacgccc caccagattg aggatatcgc cgaactgcag gctgatatcg ccactggcga
    36301 ccagttcgac cccggcacgg ttcaggcgcg agataacaac gtcatagcgt tctttaaagt
    36361 gcaggtcgcg aatacgtttt ccgagcacgt tttcattggt gaccaccaca cgctcgacgc
    36421 gcaaatcagt gcctttcgtg gacagcgaag tatcgacctc ctgaccaatc accagttgcg
    36481 cattatgtaa atccgctggc tgccccacca gatgcagcaa atcgcccagt tggataatgg
    36541 tatctggcga aggaactttt agggtttctt cgcgtttcag acgcgagcag ataattttgt
    36601 cgccgttgag aatcggtaca tctttaatag ctaaatcatg caggttaggg ttctcaacgc
    36661 gaatattgat agtcttgatc agcgcgccgc cattggtgcg tgaagactcg tgctgctgag
    36721 cttctgtctc gacattgacg cggaaaataa cccgcaacat ccacatggtg aacaaaatcc
    36781 cgcaaatgcc gaatggatac gccatcgcgt aactcatccc catctgatcg accatttcca
    36841 ttggtgtacc caggtcgcgc aagatctgct gccctgcccc cagcgctggc gtattggtaa
    36901 ccgcaccgga gaaaatcccc agcactaccg gcagtggaat atcaaacagt ttatgcagga
    36961 tggcggtaac cagaccaccg atgatgacga tcagaacagc aaacaggttg aggcgtaatc
    37021 cggagacgcg caatgaggca aagaagcccg gccctacttg aatcccgatg gtataaacga
    37081 acaggatcag gccaaattcc tgaataacat gcagcatatc gctacttaat gtcatccccg
    37141 cctgagaaac aaaatggccg acgatgatcc caccaaacag cacgccgcca atacctaatc
    37201 ctatgccgcg aaatttgacg ttgccgataa acaaaccgac gactgccacc aaagccagaa
    37261 tactgaccgt taatgctata tcactcatcg tccatttctc ttgagataac attttaattg
    37321 ctacggatta tggctgaatc ggtgaagcag atttgcctta tagcgcacat tatcacggga
    37381 aagaacgtgc cgaaatatct taaacagtcg aaatttaacg cattgttgaa gaagaaagac
    37441 aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc tagttagcta tttaacgcgg
    37501 gacgttcgct gatagcgata cgctgcgctg cgatgggttc aggctcatta cgaattaaat
    37561 caatatgcag taaaccgttt acgaaggttg cgccagagac ttccatattt tcagccagcg
    37621 taaagctcag gctaaatggc tgattcataa gcccttgatg cagccatttt ttctcttctt
    37681 ttggctgctc cggcgtgcct tttacgctca ggcgcgtacc ttccagttga atctctaaat
    37741 cttcctgacg gaaacctgcc agcgcaaggg taatgcggta gtggttatcg tcgcttttct
    37801 caatgttgta cggcgggaag ctctggcttt caccggcgtt ttgcagtgcg ttggccagtt
    37861 tgtcaaaacc gatccattga cgcatcagtg gggataaatc gaagttacgc atagtcattt
    37921 ctccttctaa gaagcgagta agtacctgca aatccgaaga ttcgcatatg ctccctgacg
    37981 gcgagcatgg agatgtcagg ccgcgtcagg cggccttagg gaattagttg atttcgatac
    38041 ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc gatatacagc aaaccattta
    38101 ccaggttagc cccacgaaca tgaatgttct cagctaactg gaatttgcgt tcaaagttgc
    38161 gttcagcgat gccctggtac agataggtgc gctctttttg ttcgtcggcg tgagcacctt
    38221 tcaccaccag cagattatcc tgggcggtaa tttccagttc gctctcagca aaaccagcca
    38281 cagcgatagc aatgcggtaa tggttttcgt ctaccagttc aacgttatac ggagggtagc
    38341 cgccattact ctggctctgg ttgttttcta agtggttaaa caaacggtca aatccaatag
    38401 cagaacggta aagcggggat aaatcaaagt tacgcataat caatagctcc tgaaatcagc
    38461 gagaatgtaa taccttccac aatggacagg tcaggtagcc agaacaccca tcaggcgcgt
    38521 cctcatcggc tacgatgtaa aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg
    38581 actttttttt gcagtttatg gtctattgca tagactgagg gggcagcacg aaacgttaaa
    38641 gtgcgatagc cgccacagtg cgacgtaatg atgatgttat tttcagcaag ggatcgctat
    38701 aactcatcag gccaacaccc acgatcaaca gatgaattta ttatgataag aaatgtgttg
    38761 ttagcgttca tgatatgcag cggaatgaca ttactcggag gatgctccag cgtgatgtcc
    38821 cataccggag ggaaagaagg aacgtatccc ggcacccgcg ccagcgcaac catgattggc
    38881 gacgatgaaa ccaactgggg cactaagtcg ctggcgattc tcgatatgcc gtttaccgct
    38941 gtgatggaca cgcttttact gccgtgggat gtgttccgca aagacagctc ggtgcgctca
    39001 cacgttgaaa aaagcgaagc caacgcgcaa gccaccaatg cggttattcc accggccaga
    39061 atgcctgata actgatttag cgatcagttt cggtgatcca cagctggccg ccttccatcc
    39121 acgccagcca ttgaccatcc ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt
    39181 ggttttcggt taaatactca acctcgccac tttgcgcatg ggcacaagca attcgattat
    39241 ccagcacaaa gcccaaccat tctcctgacg gatgccagtt aaatgcagac tgaatatccg
    39301 ttttgttatg ggttaactgg cgcggctcgc cgccctgtgg cgagataagc cacagttgca
    39361 caatgccgtt atcatcacgc attaaaaacg cgatttgcgt accctgcgga ttacagcgca
    39421 cccagtggcg cgggacgttg actaaccccg gataagcccg atggtgggta aaggttaaac
    39481 gtcgctgcac gacgccgcgc ggtggcgcgg gcagggtggt ttccgttcca cttaacggcg
    39541 catcacctgc cgctttccag ccagcttcat cttgcggtaa ctcaacgata aacagctccg
    39601 gcactttctc gccctttggc gaaagtgtgt cgccaataaa cgccagcgcg tgatttccta
    39661 cccatccttc ttcataagca cgattgattt catcgctgcc aggctgcggc gtgggcgtgg
    39721 ttttactcac cagcacgcac cagtggctac cgctgtattc acgcggatgc tgcttttgta
    39781 cgttgaccgg gccaaacggc gcagcaacac cgacgtttcg caaatccagc gccggatcga
    39841 gttcatgcat tacatggtcg ttataggtaa agctcaccct ttcaccgttc gggctaaaga
    39901 catgcacatg gctgccgccg cgcagcgctc ctggcgtata cggcgcggta atatccattg
    39961 catcgagatt gctcaccttg ccgccttcag caatgactcc gcgtcgatga tggaaatcgt
    40021 aataccatgt ttcatcagga ttttccgggc catggatgaa aacatatttc tctgacttcg
    40081 g
//
LOCUS       DAJTSL010000015        40059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTSL010000015 DAJTSL010000000
VERSION     DAJTSL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.015"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(457..2148)
                     /gene="fruA"
                     /locus_tag="OO433_000701"
     CDS             complement(457..2148)
                     /gene="fruA"
                     /locus_tag="OO433_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6049282.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAVARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(2165..3103)
                     /gene="fruK"
                     /locus_tag="OO433_000702"
     CDS             complement(2165..3103)
                     /gene="fruK"
                     /locus_tag="OO433_000702"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6049283.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(3103..4233)
                     /gene="fruB"
                     /locus_tag="OO433_000703"
     CDS             complement(3103..4233)
                     /gene="fruB"
                     /locus_tag="OO433_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6049284.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFAVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4601..5782
                     /gene="setB"
                     /locus_tag="OO433_000704"
     CDS             4601..5782
                     /gene="setB"
                     /locus_tag="OO433_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6049285.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRTKD
                     V"
     gene            complement(5779..6033)
                     /locus_tag="OO433_000705"
     CDS             complement(5779..6033)
                     /locus_tag="OO433_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6049286.1"
                     /translation="MECRPGCGACCTAPSIFSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAS"
     gene            6188..6760
                     /gene="yeiP"
                     /locus_tag="OO433_000706"
     CDS             6188..6760
                     /gene="yeiP"
                     /locus_tag="OO433_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6049287.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6983..8449
                     /locus_tag="OO433_000707"
     CDS             6983..8449
                     /locus_tag="OO433_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6049288.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMNGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8567..9553
                     /gene="yeiR"
                     /locus_tag="OO433_000708"
     CDS             8567..9553
                     /gene="yeiR"
                     /locus_tag="OO433_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6049289.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWTVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9592..10305
                     /gene="lpxT"
                     /locus_tag="OO433_000709"
     CDS             9592..10305
                     /gene="lpxT"
                     /locus_tag="OO433_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6049290.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10717..11283
                     /gene="mepS"
                     /locus_tag="OO433_000710"
     CDS             10717..11283
                     /gene="mepS"
                     /locus_tag="OO433_000710"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6049291.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11464..13020
                     /locus_tag="OO433_000711"
     CDS             11464..13020
                     /locus_tag="OO433_000711"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6049292.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGGETPEQARWLSEPGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            13102..14916
                     /locus_tag="OO433_000712"
     CDS             13102..14916
                     /locus_tag="OO433_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6049293.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADTLLNEAGWVLKGQQRVNATTGQQLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSHPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            14917..16011
                     /locus_tag="OO433_000713"
     CDS             14917..16011
                     /locus_tag="OO433_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6049294.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTILTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            16011..17036
                     /locus_tag="OO433_000714"
     CDS             16011..17036
                     /locus_tag="OO433_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6049295.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            17038..18627
                     /gene="yejF"
                     /locus_tag="OO433_000715"
     CDS             17038..18627
                     /gene="yejF"
                     /locus_tag="OO433_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6049296.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKLQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18631..18975)
                     /locus_tag="OO433_000716"
     CDS             complement(18631..18975)
                     /locus_tag="OO433_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049297.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19308..20491)
                     /gene="bcr"
                     /locus_tag="OO433_000717"
                     /pseudo
     CDS             complement(19308..20491)
                     /gene="bcr"
                     /locus_tag="OO433_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
     gene            complement(20519..21214)
                     /gene="rsuA"
                     /locus_tag="OO433_000718"
     CDS             complement(20519..21214)
                     /gene="rsuA"
                     /locus_tag="OO433_000718"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6049298.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21363..23123
                     /locus_tag="OO433_000719"
     CDS             21363..23123
                     /locus_tag="OO433_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6049299.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23248..23532
                     /gene="rplY"
                     /locus_tag="OO433_000720"
     CDS             23248..23532
                     /gene="rplY"
                     /locus_tag="OO433_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6049300.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23671..24678)
                     /gene="yejK"
                     /locus_tag="OO433_000721"
     CDS             complement(23671..24678)
                     /gene="yejK"
                     /locus_tag="OO433_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6049301.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTKFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24860..25087
                     /locus_tag="OO433_000722"
     CDS             24860..25087
                     /locus_tag="OO433_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6049302.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            25107..26867
                     /gene="pbgA"
                     /locus_tag="OO433_000723"
     CDS             25107..26867
                     /gene="pbgA"
                     /locus_tag="OO433_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6049303.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRPQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALLDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26942..27018
                     /locus_tag="OO433_000724"
     tRNA            26942..27018
                     /locus_tag="OO433_000724"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26976..26978,aa:Pro,seq:ggg)
     gene            27237..28478
                     /locus_tag="OO433_000725"
     CDS             27237..28478
                     /locus_tag="OO433_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6049304.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALISAGTDPQGHEKKKREAEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            28974..29180
                     /locus_tag="OO433_000726"
     CDS             28974..29180
                     /locus_tag="OO433_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR6049305.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLAWQKEREQEARGTAA"
     gene            29195..29860
                     /locus_tag="OO433_000727"
     CDS             29195..29860
                     /locus_tag="OO433_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049306.1"
                     /translation="MTNTKKAAPIWSRLSEQLTRCALCVYDPKHKHGDDSRYQAGGQC
                     NQSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDNVLVNSLSV
                     IVLSCIAMRRNTSHHGADSFYPLGSMPRRFSSVRPNSLSQLARLMPSLCASLSNCSFS
                     SGEIRILNCGDCPSPLGLLSRLIIDKWSPIELAFPLLGGHLNTRALKKAKPRKCHYHS
                     QGF"
     gene            29886..30086
                     /locus_tag="OO433_000728"
     CDS             29886..30086
                     /locus_tag="OO433_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6049307.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            30076..30297
                     /locus_tag="OO433_000729"
     CDS             30076..30297
                     /locus_tag="OO433_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049308.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            30290..30523
                     /locus_tag="OO433_000730"
     CDS             30290..30523
                     /locus_tag="OO433_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049309.1"
                     /translation="MRDDRFNSLKQEFPGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            30516..30749
                     /locus_tag="OO433_000731"
     CDS             30516..30749
                     /locus_tag="OO433_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049310.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            30755..31054
                     /locus_tag="OO433_000732"
     CDS             30755..31054
                     /locus_tag="OO433_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6049311.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            31051..32451
                     /locus_tag="OO433_000733"
     CDS             31051..32451
                     /locus_tag="OO433_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6049312.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPVEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVISIDGDDVAPL"
     gene            32651..32902
                     /locus_tag="OO433_000734"
     CDS             32651..32902
                     /locus_tag="OO433_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049313.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            32899..33309
                     /locus_tag="OO433_000735"
     CDS             32899..33309
                     /locus_tag="OO433_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6049314.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            33320..33592
                     /locus_tag="OO433_000736"
     CDS             33320..33592
                     /locus_tag="OO433_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6049315.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            33831..34037
                     /locus_tag="OO433_000737"
     CDS             33831..34037
                     /locus_tag="OO433_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049316.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            34037..35092
                     /locus_tag="OO433_000738"
     CDS             34037..35092
                     /locus_tag="OO433_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6049317.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQSDGKKWSEHDKDIYDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAEMACKNLGEVVSFKGYLGDIALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            35104..35439
                     /locus_tag="OO433_000739"
     CDS             35104..35439
                     /locus_tag="OO433_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6049318.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLVDNGEFK
                     PWDGQESGKAVYLTSYLVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            35452..35865
                     /locus_tag="OO433_000740"
     CDS             35452..35865
                     /locus_tag="OO433_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6049319.1"
                     /translation="MATNESIMALPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQTVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            36027..36461
                     /locus_tag="OO433_000741"
     CDS             36027..36461
                     /locus_tag="OO433_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049320.1"
                     /translation="MIDKACFVSQQEIAEHFKVNRTTIRAWTKQGMPYLNADRGKSGG
                     YHIGHTLLWSSGKSRLEAIRYHVETSALEKIMFARLLSSERDEYSSEETEHRFDEGLQ
                     IYGYSPEDVSKARNKMAGFLAGWRHAVSVRRASMEQSADTEQ"
     gene            36742..37023
                     /locus_tag="OO433_000742"
     CDS             36742..37023
                     /locus_tag="OO433_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049321.1"
                     /translation="MTEAEILGLIRRAGGISQQTDEQATQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYNRRVALYRETEKITYHWKKPERLRR"
     gene            complement(37702..>40059)
                     /gene="yejO"
                     /locus_tag="OO433_000743"
     CDS             complement(37702..>40059)
                     /gene="yejO"
                     /locus_tag="OO433_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6049322.1"
                     /translation="NGGYQYIEMNGTAEYSVLNDGYQIVQMGGAANQTTLNNGVLQVY
                     GAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGGLAIAVDQKAGGAIKASTRVM
                     EAFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLEVMDGGTATG
                     VDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAIDTIL
                     DEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANVWAGT
                     MVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSLENSV
                     WAIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNTLSGN
                     GNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDAAFTL
                     GNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAELDTV
                     RERLGSVKSVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREESSTI
                     RGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTIHGKM
                     SNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGMRADV
                     GNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNHVKVNDDGKFNNDVAG
                     TPGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT        10079 a        10188 c        10020 g         9772 t
ORIGIN      
        1 agcctataat tacgacataa tccttttcgc gcatagcaca tcctctggta aaaaattacc
       61 aaccctgtca atccacaaga atatagatac tgcaaagacg gtttaaacat gtgttcatat
      121 ttaaacatgt gctcatagca tgcatgtttt acgtaaagta aattaccgcc agagaggcaa
      181 aatgggcaga tttgagcagg ctcacaaaat gcgattaaat gcctgaatta caaaagcgag
      241 gaatttgtga cgggatgcac agaacgggaa caataaaaaa ggcagaaaat aatctgccct
      301 tgaggaaata accttagtgt ttgatgaacg tttcaagttt ctactgaaag ataaaaccat
      361 ttaaattcat aaaattacat tcacaaaccg gtcattctct ttgcgctcat caaatgttac
      421 agggcaggaa atttctgccc tgtaacacac cttttattac gctgctttcg ctactgcgtc
      481 cacttccgga cgtttcagga aggcataggc caaacccgcc accagcgtac cggcaataat
      541 tgctaccagg taacccagta ccggcgtaat agcgccaggg atcagcagaa caaacagacc
      601 accgtgcggt gccatcagtt tcgcaccaat cgccattgag attgcgccag tcagcgcccc
      661 acccacgata cagcacggca gcacacgcat cggatcacga gcagcaaacg gaattgcacc
      721 ttccgaaatg aagcacagtc ccaataccag agcggctttg ccaccttcct gctgcgcttt
      781 gtcgaatttg cgacgcgcca ccattgttgc cagacccatt gccagcggtg gcaccatacc
      841 tgccgccata atcgccgcca tcgggccata ggtttgagta ctcagcagac ccacaccgaa
      901 tgcgtacgct gctttgttta ccggaccgcc catgtcagta cacatcatgc caccgaggat
      961 cgcccccagc agaaccgcat tcgcagtccc catggtctgc agccagtgag tcagcccttc
     1021 gagaatgcca gcaactggtt taccgatcag gtagatcatc gccagaccga ccaccagact
     1081 ggaaattagc ggaatgatca ggatcggttt cagcgcctcc atactctgtg gcagtttcag
     1141 ttgcgtactg attaacttcg caatgtaacc agccaggaag ccagcaatta taccgccaat
     1201 aaagccagaa ccggtgctga ccgccagcat accgccaatc agacccggcg tgaggcccgg
     1261 acgatcggca atggagaagg caatataacc tgccagtacc ggcaccatca gcgcaaaggc
     1321 tgaaccaccg ccaatctgca tcagcgccgc tgccaacgta cccggctctt taaacgcttc
     1381 gataccaaaa gcaaaagaaa gcgcgataca cagaccacct gcgacgacca tcggcagcat
     1441 ataagagacg cctgtcagca agtgacggta agcgcctgca ctctctttct taccttcagt
     1501 ggtcgccgtt tgagctttgc ccgccggttc atacggcgtt gcttcagcaa ccgctttatc
     1561 cagttcctgc gcggttttct tcagcgccag accggtagag gtacgataca tcggtttacc
     1621 agcaaatttc gccagatcca cttcgatatc tgccgccaca atcaccagat ccgctgctgc
     1681 gacttcttca ggggtgattg cattgcccgc gccaacagaa ccacgggttt caactttcac
     1741 ccaccagcca cgttttttcg cttcggtttc aatggcttca gccgccataa aggtgtgtgc
     1801 tacgccagtc gggcaagcag tcaccgcaac tacgcgtttc ggaccgctgg cagcaaccgg
     1861 tgctgtcgca gcgaccggcg cagtgtaagg tttcgcatgg cctttggctt cactcaggaa
     1921 cagctcaggg tgcgcaactg cccgggaaat atcacccagc cagacatttt taccgttcag
     1981 cgcgctgtca ttcgggatgg aatcaccgag aacaatcgcc atttctgcgt cgttcggatt
     2041 gtcgatgatt tccagttttg cttttcgcgc caccgcgccc agcagggtct tcgccatata
     2101 ggcgcgtgcc tgaccgagat tagcgtcaat aatcagcagc gttttcatta tgcctctcct
     2161 gctgtcagtt aaaaggttgt aagtcgacgc gcgccatcat tgcggccaac tgcggacgat
     2221 cggtaatacc cacattgctt tgacttaccg ccagggctgc aacagctgtc gccagacgca
     2281 gtgtgtgttc actggattca cgcatcagca agccataaat caggccacca accatagaat
     2341 cccctgcgcc aacggtgctt acgacatcga ctgacggtgg tttggcgatc cattcgccgg
     2401 aggcattaac ccaaagcgcg ccttcggcac ccagtgaaat aacaacatgc gcaatacctt
     2461 gttcacgtag cgcatgtgca gcttcaatca catctttcat ttcaggcagt ttacggcctg
     2521 cccaaatttc caactcgcgg cggttaggtt tcaccagcca cggtgccgct ttcaaacctg
     2581 ctactaacgc ttcacggcta ctatcaaaga taatgcaagg acactgacta cgcaggcgag
     2641 tcatccagtc ggtgaacgct tccgggctga cgcctgacgg taagcttccg ctgacacaga
     2701 ccatatcgaa ctgaccgagc cagctcagag aatcagtcac aaagcgttcc cagtcggcgg
     2761 gggtgacttc aaaacccgag aagttgaagt cggtcacttc gccgtctttt tccgtcagct
     2821 taacgttaat tcgggtgcgc ccctgtacaa cctggaaacg gttggcaatg cccagctcgc
     2881 tgaacagttg ctgaaaacca tcctgattgt ctttacccag gaagccgcca acggtgacat
     2941 caattcccag gtcttttaat actttggcca cgttgatgcc tttacccgcc gcatgcagac
     3001 cggtggtttt caccaggttc acttcgccgc gttcaatttc cgggcagaaa ccaacaaggt
     3061 cataagccgg attaagggtg atcgtagcaa cacgtctgct cattatgcgc cctccccaag
     3121 accagcagcg atagcgtcgc caatggcttt cagcgcctgt tcagcatctg caccctgggc
     3181 ggtaaagcgt aggcgatgac ctttcttaac gccaagtgcc acaactttca tcagactacg
     3241 tccgtttgcc ggtttgccgg tgccatcaag gtttgtcacg gtaatatcac tgttaaattg
     3301 tttaatggta ttgaccagca tggtacctgg acgagcatgc aggccgtgtt cattgcgcac
     3361 cacaaactcc gcgcttaaca cgtcgtcggt cggcgcatca tcgctggtca gcagcgccag
     3421 caacgttgcc gcatctgctt tcagcaagcg gtcagcttta ttgtcgagca ataaatcagc
     3481 gagacgctta agaaccgcga tgggctgatc gtcattcatc gccacactca ccagcatggc
     3541 tgccgtttcg ccgtccacag caaaagcatt tgccgcacgg cttaccgcaa tcgcgctacg
     3601 cagattgcct tcggcgctat cgctcagcca gataccctgt ccgagattca gcggttgttc
     3661 attgatggct ttggtgacga aagtggcgtc aactgccccc gcctctttca gacgcgcagc
     3721 gttcagcgcc tgaagagtca gcagatcgct ggcgacgata tccagcgtca gcatttcgtt
     3781 gtcgagcttc agctgctcac tctgcttttc gcccatcagt aatgcgcgaa gttcttctgc
     3841 tgttgttgct gacttcagtt gttcagcaac ggaatcatcg ctcagtacgt gggtcagctg
     3901 gcgtagcagg cccagatgtt catccgagct ggcagcaata ccgattgcca cgtacgctac
     3961 ctgaccgtca ccccaggtga cgccttccgg gaactgaaat acctgaacgc cggttttcag
     4021 cacctgatcg cgggtgtcgg tagtgccgtg tggaatagca ataccattgc cgaggaacgt
     4081 tgaggtttgc tgttcgcgcg ccagcatgcc attgacgtag ccttctgcta cattaccggc
     4141 ctgcaccagc gccgcagcga cctggcgaat cgcctcttct ttgtctccgg ccttttcgcc
     4201 cggatggatg tcctgtacgg ataactggaa catagttctc ctctcttgct gaattgaaac
     4261 gattcagcct ctatgagaaa aaaagcgcca acctggctta gggttaaaga caagatcgcg
     4321 ctgaaacgtt tcaagaaagc ataatacttc tgtttcagca cgcaaggaaa gccgtaaagt
     4381 tatgagcaaa agtttgatgt gctgcacatt tttttgtcat ttttcctaat tgctgatggg
     4441 aaaatccgtt atcagcaatt tcatttcagc agcgtcagca gccaatcagc aacagtgaaa
     4501 tggcattttg attattcgtg gcgcttttga tttcgctgtc tgtttatttt ctgacaagca
     4561 gcgtaaactc cgcgtcttcc tcttccagtg atagacctgc atgcataact cccccgcagt
     4621 ctccagcgcg aaatcgtttg acctgacctc gacggcgttt ttaatcgttg cctttctcac
     4681 cggtattgcg ggtgctctgc aaaccccgac actcagtatt ttccttaccg atgaagtaca
     4741 tgcccgtccg gcgatggtgg gattcttctt taccggcagc gctgtcattg ggattctggt
     4801 aagtcagttt ctcgctgggc gctctgataa gcgcggcgat cgcaaatcgc tgattgtctt
     4861 ttgctgcctg ttaggcgtgc tggcctgcac cctttttgcc tggaatcgca actactttgt
     4921 tttgctattc gttggcgtct ttcttagcag ctttggctcg accgctaacc cgcaaatgtt
     4981 tgcccttgcc cgtgaacatg ccgacaaaac cggacgtgag gcggtgatgt tcagctcttt
     5041 tttacgcgct caggtttcac tggcatgggt cattggccca ccgctggctt atgcgttagc
     5101 gatgggtttc agctttacgg taatgtatct gagcgcagcg gtagcgttta ttgtttgcgg
     5161 cgtgatggtg tggctgtttt taccgtcgat gcaaaaagag cttccgctgg cgaccggcac
     5221 ggttgaagcg ccgcgccgta accgtcgcga tacgctactg ctgtttgtca tttgtacatt
     5281 gatgtggggc tcgaacagcc tgtacatcat caacatgccg ctatttatta tcaacgaact
     5341 gcatcttccc gagaaactgg ccggtgtgat gatggggacc gccgccgggc tggaaatccc
     5401 gacgatgttg attgccggat atttcgccaa acgtctgggt aagcgtttct taatgcgcgt
     5461 tgctgccgtg ggtggcgtct gtttttacgc aggaatgctg atggcgcatt cacctgccat
     5521 tctgttgggc ttgcagctgc taaatgctat ttttattggc attctgggcg gcatcgggat
     5581 gctctatttt caggatttga tgcccggtca ggcaggttca gccaccacgc tctataccaa
     5641 cacgtcgcgc gtgggctgga tcatcgcagg atcagtggcg ggcatcgtcg ccgagatctg
     5701 gaattatcac gctgtgttct ggtttgcgat ggtgatgatt atcgccactc tgttttgctt
     5761 actgcggact aaagatgttt aagacgcggt cagcatttcc agatcgataa gccaggtcat
     5821 ggcctgctga cgcgagttac cgcacatttc agcgctggct tgtaagcctg cacagacttt
     5881 cggtcgcagt ggcgaggtga aaattttaca gcgttgctgt tcatcaagct gaatgcaggg
     5941 cgtattggcg ggcttgccat ctggcatacc gggaataggg ctgaaaattg aaggggcagt
     6001 gcaacacgcc ccgcaacccg gacggcattc catacgttgt tctcttaagg ttaaccaccg
     6061 gattgatgag cgcgcacagt accacctttt ttgcaccagc aaaagtgcga ataccacttg
     6121 cccgaaaggc ccgtcgcgag tactttgtcg cgatattttt gacattttcg actacaggaa
     6181 tttttcgatg ccaagagcga acgaaattaa aaaaggtatg gtactgaatt acaacggcaa
     6241 actgctgctg gttaaggata ttgatattca gtcgcccact gcccgcggtg ccgctacgct
     6301 gtacaaaatg cgtttttctg atgtccgtac cgggctgaaa gtagaagagc gtttcaaagg
     6361 tgatgatatc gttgacaccg tgacgctgac ccgccgttac gttgacttct cctatgtcga
     6421 tggcaacgaa tatgtcttta tggataaaga agactatacc ccgtatacct tcaccaaaga
     6481 tcagattgaa gaagagttgc tgtttatgcc agagggcggc atgccggaca tgcaggtgct
     6541 gacctgggac ggtcaactgc tggcgcttga gctgccgcag accgttgatc tggaaatcgt
     6601 tgaaaccgca ccaggcatca aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt
     6661 gagcactggt ctggtgattc aggtaccaga atacttaagc ccgggcgaaa aaattcgtat
     6721 ccatatcgaa gaacgccgtt atatggggcg tgctgactaa cttcagccgc atgcagaaaa
     6781 gggatagctc aggctgcccc ttttttaatt tattatacca atcttctatt ttgcgcttcc
     6841 tgaacacctt acgccctcaa ttttcactcg ttgattgatc gccctcacac ttcatcgcat
     6901 taacaatcca gaccaatttc aattgctgtc atataacttt acactgtcat tgttaattta
     6961 tcgttactaa gacgtgactc ctatgaagac aattgcctcc gttacgctcc cgcatcatgt
     7021 acacgctcca cgctacgatc gccagcagtt gcaatcacgt atcgttcatt ttggctttgg
     7081 cgcctttcac cgcgctcatc aggcgttact gaccgatcgt gtgctgaatg cccagggcgg
     7141 cgactggggg atctgtgaaa tcagcttgtt cagcggtgat caactgatga gccagctccg
     7201 cgcacagaac catttatata ccgtgctgga gaaaggtgcg gacggcaatc aggtgataat
     7261 tgtcggtgcc gttcacgaat gccttaatgc aaaactggat tccttagcgg caattattga
     7321 gaaattttgc gagccacagg tggcaattgt ttccctgacg attaccgaaa aaggctattg
     7381 tattgacccg gccaccggtg cactcgacac cagtaatccg cggattattc acgatctaca
     7441 aacccctgaa gaacctcact ccgcaccggg tattctcgtc gaagcactga aacgccgccg
     7501 tgagcgcggc cttacaccgt ttaccgtgct ctcctgcgac aatattcccg acaatggcca
     7561 tgtggtgaaa aacgcggtgc tgggaatggc agaaaaacgt tcgccagaac tcgccgggtg
     7621 gataaaagaa cacgtcagtt ttccgggaac catggtcgac cgcattgttc cggctgcaac
     7681 cgacgaatca ctggcagaaa tcagccagca tctgggggta aatgatccct gcgcgattag
     7741 ctgcgaaccg tttatccagt gggtggtgga agataacttc gtcgctgggc gtcctgcctg
     7801 ggaagtcgca ggtgtacaaa tggtgaatga tgtcctgcca tgggaagaga tgaaactgcg
     7861 tatgcttaat ggcagccact cttttctcgc ttatctgggt tacctttcag gattcgccca
     7921 tatcagtgat tgcatgcagg atcgcgcatt tcgccatgcc gccagaacat taatgctgaa
     7981 tgagcaagcg ccgacactac aaattaaaga tgtcgattta acacaatatg cggacaagtt
     8041 aattgctcgt tttgctaatc cggcgctgaa acataagacc tggcaaatcg caatgaatgg
     8101 tagccagaaa ttaccgcaac gtatgctggc aggtattcgc atacatctgg ggcgcgaaac
     8161 ggactggtcg ttgctggcat taggcgttgc tggctggatg cgttacgtca gcggcgttga
     8221 tgatgccgga aatgccattg atgttcgcga tccgcttagc gataaaattc gcgaacttgt
     8281 tgcgggcagc agcagtgaac aacgcgtaac cgccctgctt tccctgcgtg aagttttcgg
     8341 tgatgatctg ccagataacc cgcattttgt gcaggccatc gaacaagcct ggcaacaaat
     8401 cgtacaattc ggcgcacatc aggcgctatt aaacaccctc aaaatttaac gatttctgcg
     8461 gttaaagcgg atgaagctca ccttcgtccg ctctcccctt ctcttttctg ccttttttag
     8521 ccaggattaa cgctcagtta acttaccaga gtattgcgga gccgttgtga ccaggaccaa
     8581 cctcatcacc ggttttctcg gcagcgggaa aaccacgtcg attcttcatc tgttagccca
     8641 taaagatccc aacgaaaaat ggacggtact ggttaatgaa tttggggaag tcggaattga
     8701 tggtgctttg ctcgccgata gcggcgcatt gctgaaagag atccccggcg gctgcatgtg
     8761 ctgcgttaat ggtttaccca tgcaggtagg gttgaatacc ttactgcgtc agggaaaacc
     8821 agaccgcttg ttgatagagc cgaccgggct gggccatccg aaacagatcc tcgatctgtt
     8881 aaccgcacca gtctatgaac cgtggataga tctgcgcgcc accttgtgca ttctcgatcc
     8941 acgcttgctg ctggacgaaa aaagcgccag caatgaaaac ttccgtgacc agctggctgc
     9001 cgcagacatc attgtcgcca ataaatccga ccgtgcgacg cccgaaagtg agcaagcgct
     9061 acagcgttgg tggcagcaaa atggtggcga tcgacaatta attcacagtg agcatgggaa
     9121 agttgacggt catcttctgg atttgccgcg tcgcaattta gccgagttgc ccgccagcgc
     9181 cgcgcattct catcagcatg tcgtgaaaaa agggttagca gcgttaagcc tgccagagca
     9241 tcaacgctgg cgtcgcagtc tgaacagcgg gcaaggatat caggcctgcg gctggatatt
     9301 cgacgctgat acggtattcg acaccattgg cattctggaa tgggcgcgac ttgcaccggt
     9361 ggaacgcgtc aaaggcgtgc tgcgtattcc cgaagggctg gtacgaatca accgtcaggg
     9421 cgatgacctg cacattgaaa cgcaaaacgt tgcgccaccg gacagccgta ttgagctgat
     9481 ttccagcagc gaagctgact ggaatgcctt gcagagcgcg ctgttgaagc ttcgtttagc
     9541 gactaccgcg taaggttgcc tgcgtttttc agtaagataa ttagagaaaa tatgattaaa
     9601 aatttgccgc aaatagtgtt gttgaatatt gtcggcctcg cgctgtttct ttcctggtat
     9661 attcccgtta atcatggatt atggttgccg attgatgcgg atatttttta tttctttaat
     9721 cagaaactgg tcgaaagtaa ggccttttta tggctggttg cattgaccaa caatcgcgct
     9781 ttcgacggtt gttctctgct ggcgatggga atgttgatgc tgagtttctg gctgaaagaa
     9841 aacgccccag gcagacgacg tatcgtgatt attggtctgg taatgctatt aactgcagtg
     9901 gtattaaacc agctgggtca ggcattaatt cctgtaaaac gggccagccc aacattgacg
     9961 tttactaata ttaaccgcgt cagcgaactg ctctctgtcc ccacgaaaga tgcctcacga
    10021 gatagctttc ccggcgatca cggcatgatg ctgcttattt tttcggcatt catgtggcgt
    10081 tatttcggta aagttgcagg tcttatcgcc cttattattt ttgtggtttt tgcgtttccc
    10141 agagtaatga ttggcgcaca ctggtttact gacatcattg tcggttcgat gaccgtgata
    10201 ttgatcggtt tgccctgggt gttgctgacg ccattaagtg atcgattaat cacctttttt
    10261 gacaaatcac taccgggaaa aaacaaacat ttccaaaaca aataactcac agtaattaac
    10321 atcatcaggt ttatttttat agtgaggata atcctgatga tgcgcaccgt gctttcatct
    10381 atcgaacgca aaaatcattc tctaagtaaa tgaatggatt gcatgcgttt cactcaatcg
    10441 tactttaatt gaccaatccc gcttattaac tttctgtatc actttttctt ataaaaaatc
    10501 atgtaaaacc gctcgccaag accgcaccaa tcgggtaatc tcgaactcgt tttgcctcgg
    10561 cggtagatta tcctcacagc atataatttt gtgcgttagt ccacagattt ggccttaagg
    10621 aattgtttca acatgcccag gtaattagtc tcgtgtcgct tggcattttt ttataacgat
    10681 atttgtcgtt aaggacttca agggaaaaca aacaacatgg tcaaatctca accgattttg
    10741 agatatatct tgcgcgggat tcccgcgatt gcagtagcgg ttctgctttc tgcatgtagt
    10801 gcaaataaca ccgcaaagaa tatgcatcct gagacacgtg cagtgggtag tgaaacatca
    10861 tcactgcaag cttctcagga tgaatttgaa aacctggttc gtaatgtcga cgtaaaatcg
    10921 cgaattatgg atcagtatgc tgactggaaa ggcgtacgtt atcgtctggg cggcagcact
    10981 aaaaaaggta tcgattgttc tggtttcgta cagcgtacat tccgtgagca atttggctta
    11041 gaacttccgc gttcgactta cgaacagcag gaaatgggta aatctgtttc ccgcagtaat
    11101 ttgcgtacgg gtgatttagt tctgttccgt gccggttcaa cgggacgcca tgtcggtatt
    11161 tatatcggca acaatcagtt tgtccatgct tccaccagca gtggtgttat tatttccagc
    11221 atgaatgaac cgtactggaa gaagcgttac aacgaagcac gccgagttct cagccgcagc
    11281 taataaaccg tttggatgca atcccttggc tatcctgacg agttaactga aagcactgct
    11341 taggtagtgc ttttttgttt tcattcatca gagaaaatga tgtttccgcg tcttgatcca
    11401 ggctatagtc cggtcattgt tatcttttaa atgttgtcgt aatttcagga aattaacgga
    11461 atcatgttca tacgcgctcc caattctgga cgtaagctcc tgcttacctg cattgtagca
    11521 ggcgtgatga ttgcgatact ggttagctgc cttcagtttt tagtggcctg gcataagcac
    11581 gaagtcaaat acgacacact gattatcgac gtacaaaagt atctcgatac ctattttgcc
    11641 gacctgaaat ccactactga ccggcttcag ccgctgacct tagatacctg ccagcaggct
    11701 aaccccgaac tgaccgctcg cgcggcgttt agcatgaatg tccgtacgtt tgtgctggtg
    11761 aaagataaaa aaacattctg ttcatctgcg actggtgaga tggacattcc actcaatgaa
    11821 ttgattccgg cgctcgacat taataaaaac gtcgatatgg cgatcttacc cggcacgccg
    11881 atggtgccga acaaacccgc aatcgtcatc tggtatcgca accctttgct gaaaaatagc
    11941 ggtgtgtttg ccgctctgaa tctcaacctg acgccttctc tcttttatag ttcacggcag
    12001 gaagattacg atggcgtcgc cctcattatt ggcaatactg cgctatctac cttttcttca
    12061 cgtttgatga acgttaatga attaaccgac atgcctgtcc gtgaaactaa aattgcgggc
    12121 attcctctga ccgttcggct ttatgcggat gactggacat ggaacgatgt gtggtacgca
    12181 tttttactgg gcggcatgag tggaactgtc gttggcctgc tctgctatta cctgatgagc
    12241 gtacgtatgc gccccggcag agaaatcatg accgccatca agcgcgaaca attttacgtg
    12301 gcgtatcaac cggtggtgga tacacaagct ttgcgagtaa cgggcctgga agtactgcta
    12361 cgctggcggc atcctgtcgc gggagaaatt cccccggatg ccttcattaa ctttgccgaa
    12421 tcgcaaaaga tgattgtgcc gctgactcag cacctgtttg agttaattgc ccgcgatgcc
    12481 gcagaattag aaaaagtgct gccagtaggc gtcaaatttg gtattaacat tgcgccggac
    12541 catctgcaca gcgaaagctt taaagcagat atccagaaac tgctcacttc cctgcccgca
    12601 caccatttcc agattgtgct ggaaattacc gagcgcgata tgctgaaaga gcaagaagcc
    12661 acacaactct tcgcctggct gcactcggtc ggcgtagaaa ttgctattga tgacttcggc
    12721 accgggcaca gcgcgcttat ctatcttgag cgttttacgc tcgattatct gaaaattgac
    12781 cgtggattta tcaacgccat cggtacggaa acgatcactt cacccgtact tgacgcggtg
    12841 ctgacgctgg cgaaacgcct caatatgctg acggttgctg aagggggcga aacgccggaa
    12901 caggcgcgat ggctaagcga acccggcgtt aatttcatgc aaggctactg gattagtcgc
    12961 ccgttaccgc tggacgattt tgttcgctgg ctgaagaaac cgtatacgcc gcagtggtaa
    13021 ggtgtgctta cgtcccttat tattcatagt gaaagcatgc cggattgcgg ctaatgatga
    13081 gtaaaaggaa atccgttgca gatgattgtg cgcatactgc tgctgtttat cgccctgttc
    13141 accttcggtg cgcaggcgca ggctatcaag gaaagctatg cctttgccgt gctgggcgaa
    13201 ccccggtacg catttaattt caaccatttt gattatgtga accccgccgc gccaaaaggt
    13261 gggcagataa cgttgtcagc cctcggtacc ttcgataatt tcaaccgcta tgcactgcgc
    13321 ggcaacccgg gcgcacgcac cgagcagttg tacgacacgc tatttacgac ttccgatgac
    13381 gaaccaggca gttattaccc gctgattgct gaaagtgcac gctatgctga cgattattcc
    13441 tgggtggagg tcgctattaa tccgcgcgcc cgttttcatg atggttcgcc cattactgcc
    13501 cgcgatgtag agtttacttt tcaaaaattt atgaccgaag gcgtgccgca atttcgtctg
    13561 gtctacaaag gcaccaccgt caaagccatt gcaccgttaa ccgtgcgcat tgagttagct
    13621 aaacccggca aagaagatat gctgagtctg ttttcgctgc cggtatttcc agaaaagtac
    13681 tggaaggatc acaaacttag cgacccgctc gccacgcctc cgcttgccag tggtccgtac
    13741 cgcgttacgt cctggaaaat ggggcaaaat attgtctatt cccgtgtgaa agattactgg
    13801 gcagcaaact taccggtaaa ccgtggacgc tggaatttcg acaccattcg ctacgattat
    13861 tacctcgatg ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg
    13921 gaaaacgacg ctaaaaactg ggccacgcgt tataccggta aaaatttcga taaaaaatac
    13981 atcatcaaag atgagcaaaa gaacgaatca gcccaggata cgcgctggct ggcgtttaat
    14041 atccaacgtc cggtattcag cgatcgccgg gtccgggaag caatcactct cgcctttgac
    14101 tttgaatgga tgaacaaggc gttgttttac aacgcctgga gtcgcacgaa cagttatttt
    14161 cagaataccg aatacgcggc cagaaattac cccgacgccg cggagctggt gcttctggca
    14221 ccaatgaaaa aagatctacc gccagaagtc ttcacacaaa tctaccagcc gccggtatct
    14281 aaaggcgatg gctacgatcg tgacaacctg ttaaaagccg acacacttct caacgaagcg
    14341 ggctgggtgc tgaagggtca gcaacgcgtt aatgccacaa cgggtcagca actcagcttt
    14401 gaattattgc ttcccgcaag cagcaatagt cagtgggtat tgccgttcca gcacagcctg
    14461 caacggctgg gtatcaacat ggacattcgc aaggtggata actcgcaaat caccaaccgc
    14521 atgcgcagtc gcgactatga catgatgccg cgcctatggc gggcgatgcc gtggcccagt
    14581 tccgatttac agatttcctg gtcatcggaa tatatcaatt ccacttataa tgcccccggc
    14641 gtgcaaagcc cggttatcga ctcactgatc aaccaaatta ttgccgcgca gggaaataaa
    14701 gaaaaattat tgccgttggg gcgagcactg gatcgcgtat taacgtggaa ttattacatg
    14761 ctgccaatgt ggtacatggc ggaagaccgt ctcgcctggt gggataaatt ctcccacccc
    14821 gctgtacgcc ctgtttacag cctgggtatc gatacctggt ggtatgacgt taataaagcg
    14881 gccaaactgc cgtcagccag gcaacaggga gagtagatgg gcgcttacct gattcgccgt
    14941 ctgttgctgg tgatcccaac attatgggcg attatcacca tcaacttttt catcgtgcaa
    15001 attgcgcctg gcggtccggt cgaccaggcc atcgccgcca ttgagtttgg taatgccgga
    15061 gtattacccg gcgcaggcgg tgaaggtgtt cgtgccagcc atgcgcaaac gggtgtcggc
    15121 aatatcagcg acagtaatta ccgtggcgga cgcggattag atccagaagt gatcgctgag
    15181 atcactcatc gctacggttt tgataagccg atccacgaac gttacttcaa aatgctctgg
    15241 gactacatcc gctttgattt tggtgatagc ctgtttcgca gcgcctcggt gctgacgctg
    15301 attaaagaca gtctgccggt ttccatcacc ctcggattgt ggagcacgct gattatctat
    15361 ctggtgtcga ttccgttagg cattcgcaaa gctgttcata atgggagccg ctttgacgtc
    15421 tggagtagcg catttatcat tatcggctac gccattccgg cctttttgtt tgccatcctg
    15481 ctgattgtct tcttcgcggg cggcagctat ttcgacctgt tccctctacg cggcctggtt
    15541 tccgctaact ttgattcgct gccgtggtat cagaaaatca ccgattatct gtggcatatc
    15601 acgctgccgg tgctggcgac ggtgattggt ggctttgcgg cgctgaccat actgacaaaa
    15661 aactcattcc ttgatgaagt gcgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta
    15721 agtgaaaaaa atattctctg gaaacatgtg ttccgcaacg ccatgctgct ggtgattgcc
    15781 ggttttccgg cgacgtttat cagcatgttt tttaccggct cgctgctgat tgaggtgatg
    15841 ttttcactca atggtctggg cttactgggc tacgaagcga ccgtctcgcg cgattatcct
    15901 gtaatgtttg gtaccttgta tattttcacc ctgattggcc tgctgctgaa tattgtcagt
    15961 gatatcagct atacgctggt tgatccgcgt attgattttg agggacgtta atgtcgcgac
    16021 tcagccccgt caatcaggcc cgttgggcgc gttttcgtca taaccgtcgc ggctactggt
    16081 cgttatggat tttcctcgtc ttgtttggtt tgagtttgtg ttctgagctt atcgccaacg
    16141 ataaaccatt gctggtacgt tatgacggca gttggtattt cccgttgttg aaaaactaca
    16201 gcgaaagcga ttttggcggc ccgctggcaa gtcaggctga ttatcaggac ccgtggctga
    16261 aacaacggct ggaaaataac ggctgggtac tgtgggcacc gattcgcttt ggtgctacca
    16321 gtatcaactt tgctaccgat aagcccttcc cttctccacc ctcccggcaa aactggctgg
    16381 gaacggatgc caacggcggc gatgtgctgg cacgcattct ctatggcacg cggatctcgg
    16441 ttctgtttgg cctgatgctg actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc
    16501 tacaaggcta ttacggcggt aaagtcgatc tctggggaca acgctttatt gaagtatggt
    16561 cggggatgcc gacgctgttt ttgattattt tactttccag cgtcgtacag cctaacttct
    16621 ggtggctgct ggcaattact gtcttgtttg gctggatgag tctggtcggc gtggtgcggg
    16681 cggagttttt acgtactcgt aatttcgact acattcgtgc ggcgcaggcg cttggcgtca
    16741 gcgatcgcag tatcatcctg cgtcatatgt tgcctaatgc catggtcgcg accctcacct
    16801 ttttaccgtt tattttatgt agttcgatca ccaccctgac ctcactcgat ttcctcggct
    16861 tcggtctgcc gctcggttca ccgtcactcg gtgaactgct gttacaaggg aaaaataacc
    16921 ttcaggctcc gtggcttggg atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc
    16981 tgatctttat tggtgaagcc gtccgcgacg catttgatcc taataaggcg gtgtagcatg
    17041 acgcaaactc tgttagcgat tgaagatttg tcggtgggtt ttcgccatca gcaaaccgta
    17101 cgtacagtag tcaatgatgt ttcactacag attgaggctg gcgaaacgct ggcgctggtg
    17161 ggtgagtcag gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc
    17221 ccgccggttg aatatctctc cggcgatatt cgttttcgtg gcgaatcgct gcttcatgcc
    17281 agcgatcaaa cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt tcaagagccg
    17341 atggtgtcgt taaatccatt gcataccctg gaaaaacagc tttatgaagt gctttcactc
    17401 caccgcggga tgcgtcggga agcggctcga ggcgaaattc ttaactgcct tgatcgcgtt
    17461 ggtatccgcc aggcggcaaa acggctgaca gattatccgc atcagctctc cggcggcgaa
    17521 cgccagcggg tgatgattgc gatggcgctg ttaacgcgac cggaattatt aattgccgat
    17581 gaaccgacca ccgcactgga cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa
    17641 ctgcaaggcg agctgaatat gggcatgctg tttattactc ataacctcag cattgtcaga
    17701 aaactggccc accgcgtggc ggtaatgcaa aacggtcgct gtgtcgagca aaattacgcc
    17761 gctacgctat ttgcctcacc cactcatcct tacacacaaa agctactcaa cagtgaaccg
    17821 tcaggcgatc cagtgccgtt gccagaacct gcctcaacgt tgctggatgt tgaacagctt
    17881 caggttgcct tccccattcg caaagggatt ttgaagcgca ttgtggatca taatgtggtg
    17941 gtgaaaaaca tcagttttac gctacgagcg ggtgaaacac tgggtttagt gggcgagtcc
    18001 ggttccggga aaagtacgac gggactggcg ctgctgcgac tgattaattc tcagggcagc
    18061 atcatctttg acggtcagcc actgcaaaat ttaaatcgcc gccagctgtt acctattcgt
    18121 catcgcattc aggtggtatt tcaggatcca aactcctcgc tcaacccacg actcaacgtt
    18181 ttgcagatta ttgaggaagg cttacgggtt caccagccga cgctttctgc cgcacaacgc
    18241 gaacaacaag tgatagccgt gatgcatgaa gtgggattag atcctgaaac acgccaccgt
    18301 tatccggcgg agttctctgg tggtcagcga caacgtattg caattgccag ggcattaatt
    18361 cttaagccct cgctgatcat acttgatgaa ccgacatcat cactcgacaa aacggtacag
    18421 gcgcaaatat tgacgctatt gaaatcattg caacaaaagc tgcaactggc ctatttgttt
    18481 atcagccacg atttgcacgt tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa
    18541 ggggaagtag tggaacaagg accgtgcgcg cgcgtgtttg ccgcaccgca gcaggagtat
    18601 acgcgtcagc tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc
    18661 ggcaattgcc acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt
    18721 tacaaacagg cacggctcac cttcacactc cagcaccgaa cacggaactt cgatgtcgct
    18781 taacgcctgg cttagtttgt acatcaccga ccatgcatta tcaccatccg ggctaagcaa
    18841 tttaagcgcc accaggctgt tgtcaccgca cggtccattt tgcggaatcg gttcaacctt
    18901 cgaacctgcg aaaccagcag accagcgaaa gttctgcggt agacgatgga cgattgagag
    18961 ttgtaatgaa gtcacttatt ttccccggaa gcacatttac ttcacaattt gtttacatca
    19021 attttaacac atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct
    19081 gcgtggcctc tatggctctg atttaagtaa tatctgcggg cgtggtcacg tttgcggggc
    19141 tatatgtacc cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt
    19201 acataaattg attttacata aaataaacat atatcggggg aatgatagat ttgtggttga
    19261 catgcatcaa caaaggaagc cttttagctt cctcgttgtg caatagatca ccgttttttc
    19321 ggtcgactgg cgtacagaca gaagagaatg gagctgcttg cgcagaatgc aattgaccaa
    19381 atcatcggcc atgcagagtt aaaggtcgca agagaaagca atgcgccaac aattgcccct
    19441 atgccaaaac ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca
    19501 tcaagaatga ccgccatcgc attggatgac accatcgaca cacagcccac aaacgccgca
    19561 acgccaacca ccagaatccc agccccagca gcgcactgat gaccatccac gctgccataa
    19621 taaattgtat ccacaacccc gagcggaaca tatttaacgc gccaatgcgg cgaacgaagc
    19681 ggctgttaaa gatggtcatc acgaacagga aaacaatgtt tagtgcaaag taataaccaa
    19741 agttttccgg cgcgacgtgg ttaatttcaa tgtagacaaa cggtccggcg ctcaggaacg
    19801 agaacattcc ggcaaagctg aaaccgctgg ccagcatata actcaggaca cgcttatgac
    19861 ggaacagcgc cgcaaagtta ccaatagtag tacgaatgtg aaaaggctgg cgacgctccg
    19921 gtggtaaggt ttctttgatc agaaagaaga tcattgccga agccagaatc gccgccacgg
    19981 cgaggatcca gaagatgtaa tgccagctca gccacaccag cacccagccg ccaactatcg
    20041 gtgccatcag cggtgcaatg gtcgtcacca gcatgacaaa cgacatcatc cgcgagaact
    20101 cttctttcgg gtagatatca cgcatcaaag cgttgataac catgctggcc gcagccgcag
    20161 ccagcccgtg gaaaaaacgc atcacaatca gctgatcgat ggtttgtgcc aacgcacacg
    20221 ccaccgcggc ggcggcaaac accagcgtac cgccgagcac caccggctta cgcccgaagc
    20281 tgtctgccat cggcccgtag attaactgcc ccaacgcaaa gcccagaata taagtactga
    20341 gggtcatctg cgtactgccc gccggtacgc caaactgcgc tgaaattacc ggtagcgcgg
    20401 gcagatacat atcaatcgac agcggcatca acatggccag caggccaagg ataaaaacaa
    20461 tagcaaacga cgaatgctgt cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt
    20521 agacgacgct ggcaatttct tcttcagtta acggacgata ttcaccgggg gctaaatcag
    20581 catccagcgt aataccgcca atacgttcac gatgcagctc aaccacgtgg ttacccacgg
    20641 cggcgaacat gcgtttcacc tgatgataac gcccttcgct gatggtcaga cgaacctgcg
    20701 ttggggtaat cacttccagc accgcaggct tagtgagatc tttttcgtta tgcagctgca
    20761 cgcctttagc aaattgctct gccgtatcgt cagctacagg tgattccagt gtcaccagat
    20821 aggtcttctc gcaatgatgg cgcggagaag taatgcggtg cgaccactga ccatcatcag
    20881 tcatcagcac cagaccggtg gtatcgatat ccagacgtcc tgcggcgtgc agtttccagg
    20941 ctaccggttc atccagaaaa tagagcaccg ttgggtgatc agggtcgtcc gtggagcaaa
    21001 catagccctg aggcttattg agcatgaagt aacgtggacc atgttgctgc gccagcgggt
    21061 tgccatcgta agcgacatca tgttcaggaa gcagtttgaa cgctgcatta cgaacaattt
    21121 cgccatcgac ggtgacacga ttgccgcgga tttcacgccc ggcaatagca cggctaacgc
    21181 cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct gtaaaaacgg
    21241 aggtcgggca ttgacccgaa aatctgaact gttgtctgcc cagtatagcg gtctaataac
    21301 atccctcaag ggaaaaagat ccatggcata ctattagcag aataatctac ctacgcgaga
    21361 ccatgatttt tacactccgc ccatatcagc aagaagccgt ggatgccacg ctcaaccatt
    21421 ttcgtcgtca taaaacccct gccgttatcg tgctgcccac cggcgcaggt aaaagcctgg
    21481 tgattgccga actggcacgg ctggcgcgtg gtcgtgttct ggtgctggca cacgtcaaag
    21541 aactggtggc gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc gatatttttg
    21601 ccgctgggtt aaagcgcaaa gagagccacg gtaaagtggt atttggcagc gtgcagtcgg
    21661 tcgctcgtaa tcttgatgcc tttcagggtg aattttcgct gttgattgtc gatgaatgcc
    21721 accgtattgg tgacgatgaa gagagccagt atcagcaaat cctcactcac ctgactaaag
    21781 tgaatcccca cttacgcctg ctggggctga ctgccacgcc ttttcgattg ggcaaaggct
    21841 ggatctacca gttccattat cacggcatgg tacgcggcga tgagaaagcc cttttccgtg
    21901 actgcattta tgagctgccg ctgcgttata tgattaaaca cggctatctg acgccgccag
    21961 aacgactgga tatgccagta gtgcaatacg atttcagccg cttgcaggca cagagtaacg
    22021 ggctgttcag cgaagctgat ctcaaccgtg agctgaaaaa acagcaacgc attacgccac
    22081 atatcatcag ccagatcatg gagttcgccg aaaagcgcaa aggggtaatg attttcgccg
    22141 ccaccgttga acacgcaaaa gagattgtgg gattactgcc tgccgaagat gcagcactga
    22201 ttactggcga cacccccggc gctgagcgcg atgtgttaat tgaagatttt aaagcccagc
    22261 gttttcgcta tctggtcaac gtcgcggtac tgaccaccgg atttgacgcc ccgcacgtcg
    22321 atcttatcgc cattctgcgt cctaccgaat cagtgagtct ttaccaacaa attgtcgggc
    22381 gcggtctgcg tctcgctcct ggcaagactg attgcttaat tcttgattat gcgggtaatc
    22441 ctcacgacct ctacgcgccg gaagttggta caccgaaagg caaaagtgac aacgtcccgg
    22501 tacaggtttt ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa acgaccgccg
    22561 acgggacatt gattgaacac tttggtcgtc gctgtcaggg atggtttgaa gatgacgacg
    22621 gccatcgcga acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcgg
    22681 aaaacgatat tgccgcccgc cgctgccgcg aatgtgacac ggtactggtt gatccggatg
    22741 atatgttaaa agcggcgcta cgactgaaag acgcgctggt attacgctgt agcggcatgt
    22801 ctttacagca tgggcacgac gagaaaggcg aatggttgaa aatcacctat tacgatgaag
    22861 acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc cgcccagcga actgccttcg
    22921 agcagctttt tatccgcccg catacgcgca caccgggcat ccctctgcgc tggatcaccg
    22981 ccgccgatat cctcgcccag caagccttac tgcgacatcc ggattttgtc gtcgcccgca
    23041 tgaaaggtca gtactggcag gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc
    23101 gggcgcacga attacgcggt taatggtagt tttgattgat gtataaggcg attgagtata
    23161 gaatctcgcc cgcttttgca tacgcaaagc agatcactta cctgttgctg ggtcgcctgt
    23221 agcaggatta atttaagaga gaaagaaatg tttactatca acgcagaagt acgtaaagag
    23281 cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc aatcatctac
    23341 ggtggcaaag aagcaccgct ggctatcgag ctggatcacg acaaagtcat gaacatgcaa
    23401 gctaaagctg aattctacag cgaagttctg accatcgttg ttgacggtaa agaaatcaaa
    23461 gttaaagctc aggacgtaca gcgtcacccg tacaaaccga agctgcagca catcgacttc
    23521 gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc tccggcgggg ttttttgtat
    23581 ctgcagatta tgcctgatta cggtattgct attttttgca ggccagataa ggcgttcacg
    23641 ccgcatctgg cattcagaat acaaaaccga ttaattgccg ccagacgtcc ggcgctgcaa
    23701 ttggtcgcgc aaattcggcg gcgtgccttt aatggtcaag gtatcggttg ctggatccca
    23761 gaagatgcgt tcacctaaca gcatcgcatc aaagttaata gtcaagccac cgccgctacc
    23821 ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc gggaagctct cttccagttc
    23881 gtagcctttt tcagccgcaa acttcgtgaa gctcacttcg cttacgcctg ccagctcttt
    23941 cgacagcgat tccagttcga tctcttcgcc tgcctgcagc tgctcattac agtagctata
    24001 aacctgctgg cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc
    24061 atcaaccgct tgcaacagcc cacggttttg cgctttcgcg ttcaatcctt cgctggcccc
    24121 gaggaaatcc ataaagaaat ccgccacttt gcgccctact cgccctttca ggaaagtgag
    24181 atagcgggtg gactctggat tggtttccca ttcggttaaa tcgatacgcg caacaatatc
    24241 cgcatgattg atatcaagat aatgggttgg gttgatgtcg agattttcgt taacgcgcat
    24301 actgctcagg ttgctcagaa ctgccaccag cagatactcc accgccagat aacggtagtg
    24361 gcaaaacagc acgaatccac cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg
    24421 cccggttgcc gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct gtaagcgcag
    24481 cgtctgcgcc agttcactct cttcgctaaa caagccgtaa gctttatttt tggcgctata
    24541 gacccgatgc agttctgcca ccatctcaac gacggtttct gtcggttcca gcaatgaatc
    24601 gcgcaacacc agctcaaggt tctgctcatc acgcttgata agctggtgca gagcaatctg
    24661 gttgatatcc agactcatga taaactctcc tttaagaccg ggcggtattc aaccaccgcc
    24721 ggttaagcga cgcaataaaa gatggcggtc attttacaga aggcagcgta acgagaatga
    24781 tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg ccctttcatc
    24841 aactaactga tttcgattta tgccacaaat ttcccgctac agtgatgaac aggttgaaca
    24901 attgctcgca gagctgctca acgtactgga aaaacataag gctccgaccg acctttccct
    24961 gatggtgtta ggcaacatgg tcactaacct tatcaacacc agcattgccc cggcccaacg
    25021 ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc tctatcaacg aagacaaagc
    25081 gcactaaggg aaacagataa caggttatgg taactcatcg tcagcgctac cgtgaaaaag
    25141 tctcccagat ggtcagttgg gggcactggt ttgcactgtt caatattctg ctttcgctcg
    25201 tcattggcag ccgttacctg tttatcgccg actggccgac aacgcttgct ggtcgcattt
    25261 attcctacgt aagcattatc ggccatttca gcttcctggt gttcgccacc tacttgctga
    25321 tcctcttccc gctgaccttt atcgtcggct cccagaggct gatgaggttt ttgtccgtca
    25381 ttctggcaac ggcgggaatg acgctattac tgatcgatag cgaagtcttt actcgtttcc
    25441 atctccatct taatcccatc gtctggcagt tggttatcaa cccagacgaa aatgagatgg
    25501 cgcgcgactg gcagctgatg ttcatcagcg tgccggttat tttattgctt gaactggtgt
    25561 ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc ttcgcgcgcc
    25621 cgctggccgc attcttattt atcgccttta tcgcctcgca tgtggtgtat atctgggccg
    25681 atgccaactt ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt tcgtacccga
    25741 tgacggcgcg acgtttcctt gagaagcatg gcctgcttga tgcgcaggag tatcaacgcc
    25801 gtcttattga gcaaggtaat ccagacgccg tttccgtaca gtatccgtta agcgaactgc
    25861 gctatcgcga tatgggcacc gggcagaatg tgctgttgat tactgtcgat ggcctgaact
    25921 actcacgctt cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa aatatttcgt
    25981 tcacgcgcca tatgagctcc ggcaacacta cagacaacgg catctttggc ctgttctatg
    26041 gcatctcgcc gagctatatg gacggcattc tgtcgacccg tacccctgcg gcgttaatta
    26101 ctgcgcttaa tcagcaaggc tatcagctgg gattattctc gtcagatggc tttaccagtc
    26161 cgctgtatcg ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc ccccaatccg
    26221 acgagcagac cgccacgcag tggatcaact ggctgggccg ctacgcacaa gaagataacc
    26281 gctggttctc gtgggtctct ttcaatggca ctaacattga cgacagcaat cagcaggcat
    26341 ttgcacggaa atatagccgg gcggcaggca atgtcgatga ccagatcaac cgcgtgctca
    26401 atgcactgct tgattctggc aaactggaca atacggttgt gattatcact gccggtcggg
    26461 gtattccgct gagcgaagac gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc
    26521 cattagtgat tcactggcca ggcacgccgg cgcagcgtat taatgcgctg actgatcata
    26581 ccgatctgat gacgacgctg atgcaacgcc tgctacatgt cagcacacct gccagcgaat
    26641 attcgcaagg tcaggatttg ttcaaccctc aacgccgtca ttactgggtc actgcagcgg
    26701 ataacgatac gctggcaatt accaccccga aaaagacgct ggtgctgaac aataacggta
    26761 aataccgcac ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt
    26821 tgttattgca agtgctgaca gacgagaagc gttttatcgc taactgatta attataaatc
    26881 agttagcgaa atatcttact tgcaatcggt gtggaaaacg gtagtattag cagccacgag
    26941 tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa
    27001 atcctctcgt gccgaccaaa tcgcccaccg gataccaccg gaattaacag tttttagaca
    27061 cgaaaagccc gctaatcctt aattagcggg tttttttatg gtttttagtc accggacatc
    27121 accggattta taagctatca gccggatttt ttagttatac gcttagttat acgggcttgc
    27181 gtataactaa agccgtataa ctaaaaccct gttttttcgt gtggaatcat atacatatgg
    27241 cgagaaaaac acccccatta accacggtgc agatcaaagc agccagacca gcggaaaagg
    27301 aatacaccct acaggacggc ggagggcttt ttctcctggt caaaccgtcc ggatcaaaac
    27361 tctggcgatt ttcctactac cggccatcgg acaaaaaaag aatattgctg agttttggat
    27421 cgcttgatga tgtttccctg gctgatgcca gaaaacgccg tagcgagtac agagcgttaa
    27481 tcagtgccgg aactgacccg cagggccatg agaagaaaaa acgagaggca gaggcccgaa
    27541 gacaagggaa cacgttcgaa aatgtggcgg cggcatggta ccaggtgaaa atcagccaga
    27601 atctggcccc caacacgatt aaagacatct ggcgttcgct ggataaatat gtattcccgt
    27661 tcatcggcaa cacgccaata gataccctca ccgcccgaag gttcgttgaa gtgcttacac
    27721 ccatcaagga gcgcggcaac ctggaaacac tcaagcgggt tttacagcgc gttaatgagg
    27781 taatggatta cgccgccaac agtgggctga ttgatgccaa tccggctatg aatgtgcgta
    27841 aggcgtttcc ctcacctgta aaaaaacata tgccaacaat ccgccccgaa cagctgccgg
    27901 agcttatgca ggctttatca gtatcggcaa cagaacggca gaccagatta ctgattgaat
    27961 ggcagttact gaccgtaacc cgtcccgccg aagcgtcatc aacgcggtgg gatgaaatca
    28021 acctggacgc gaagcaatgg acgatacctg ccggacgcat gaagatgcgc agggatcacg
    28081 ttatcccgct ttccggtcag gctatggcgg tgctggaggc catgaaacca atcagccacc
    28141 accgcaatta cgttttccca agtctgaaag acccacagca gccgatgaac agccagacag
    28201 ctaacgcagc attgcggcgt atgggattcg ctggcgtgct ggtgtctcat ggattacgcg
    28261 ccatattcag cacagcagcg aacgaggaag gattcgagcc ggacgtaata gaggcggcac
    28321 ttgcccacgt cgacaccaac gaagttagac gggcatacaa ccggagcaac tacatagaaa
    28381 aacgcatcgt gctgatgcgc tggtggggcg aatttgtcga ggctgcggcg acgggcgtaa
    28441 ccctcgccag tggtaaaagg ggtatccgag ccgtgtagct gtacagaaaa ccagtaaaaa
    28501 ctacgaaaac catgtaaaac cgtcgtataa ttgcatcaaa tttaacgaca aggccgtgaa
    28561 acatgaaacc gttaagatgc aaaaaaatat cagatgcaat tgcgacgggc tgcaactggc
    28621 cctgatggtt cagcatgaat tttggtcaac ctacgatccg gaggacagaa cgacggcccc
    28681 atcaaaaaaa caggtagtag attttctggt atcccgtggc gcttccagaa atctggcggt
    28741 aagtattgat aaggtcgtac gtccggcatc tatgaagatc ggaggcaggc ccaaaaaatg
    28801 gcggtaacaa tcctggaagc ggcagaaatg ccgctttttt tataattcca tttcaaatca
    28861 tcaatataaa aacggtgtat accgtttaaa aacggtggga actgttttta cccatatccg
    28921 atgatttacc gtatttgtca ccggaataca ccggattcac aaggtaaatc acgatggaag
    28981 caatcagaaa aattatcttt cgccaggaag taaaaaaaat tatccacatc aaggcagaca
    29041 gcacgctgca aagcatgatc aacgccggag aatttccgca gggttttcgc gttggtttac
    29101 gccgtcgcgg atggtatgag gatgatgtgt tggcctggca gaaagaacgc gaacaggaag
    29161 cacgcggaac ggctgcttaa cgggtatcac agatatgaca aacacgaaaa aagcggcccc
    29221 gatatggagc cgcctttctg aacaattaac ccgctgcgcc ttatgtgtat atgatcccaa
    29281 acataagcac ggggatgata gccgctatca ggctggtggg caatgcaatc agtctggttc
    29341 agttcgttgc catacctgca atgagcgctt ttccctatac tctttaagga attgctcaag
    29401 ggcaaaagca catggtgcga atctttctga ttcatgctct atctttctgc gccgtctttt
    29461 ccgtgccggt gataatgttt tggtcaattc tttatcggtc attgtgttgt cctgcatagc
    29521 aatgcgccgt aatacctcac accacggcgc tgatagtttt tatcctttgg gttctatgcc
    29581 gcgccgcttt agttcagtgc gccccaactc tttaagccag ttggctaggc ttatgccgtc
    29641 gctctgtgct tctttgtcga attgctcttt tagctctgga gaaattcgca ttctgaattg
    29701 tggggattgc ccgtcccctt tagggctttt atcgcgtttg attattgaca agtggtcacc
    29761 tattgaatta gcctttccat tgttaggtgg ccaccttaac acaagagcac ttaaaaaagc
    29821 aaagccccgc aagtgtcatt accactcgca gggcttctaa ccaacaacgt aaactaggag
    29881 ccgttatggt tgccgtaaat catataccac accttgtaca cacacaaacg gcctttgtgt
    29941 ggcgttttct ggccctgagt gccggagaat ctcaaatcat ccacgtaacc gcctggacgg
    30001 aacgcgaagc gcgtagccgt tgcccgtccg gttgtgttgc tgtattcgcc gcccgtattc
    30061 gtcaggggga aacctatgca caataaaacc acaccgaacg cagcagccgc cgcactcact
    30121 acgctgatgc acgcgcttat tgatattgaa tgcactgcag agcttgcgca gggagaagaa
    30181 cagaaagacc ggacacagtt cgccctggaa tgtatccgat acatcgcaac gcggtcgctg
    30241 aatgacgcta aaaatattct tgttgctgat tgtgaaaatg gggggggtta tgcgtgatga
    30301 tcgttttaat tccctgaaac aggaatttcc cggcgttcct gatgatgcgg ctgatgcgct
    30361 ttcgtcaatg ccagaactta ttagagcggc ttttttctta ctttccacga gagaatataa
    30421 atcaacgggg cttgatgtac tgaatatcgc cgccgattat gcggaatatg tggcagaggc
    30481 gcgttacaga agaaaatttc ctgaggatgt aagccatgcg tgatatttac cacgaaacaa
    30541 tagaccgcgc atttcttgca ctttctcaca gtgaaaacat gctggaaata ttgcgcatat
    30601 ggcttgaaac acttggcgac aatgaacgcg acaaacaaaa atcaagaatt gccacggcat
    30661 taataacgct tcttgagcct gtaataatgg aactgcaaga aatagatcta ttgcacgaca
    30721 gatataaaga acagcacacc ggagaataaa aataatgaaa cttaaatatt ctggcttaac
    30781 tgccagtggc aacactcacc ctaaatttac gcgcggtgat atttaccgcg accagtacgg
    30841 cggcacggta atgattaagg gcgtggaaga acggcgtgta acctaccgcc gtgaaggtta
    30901 cgaatatgat tgcgtgatgc ctgtttatca gttccggcgt gatttttctc tggtacagac
    30961 cgcgccgcat aacgtgccca ccagcaacgc cagggcacgg gcaaacatcc agaagctgaa
    31021 aaccatgatt aacggattca ggggcaagaa atgaaaagcg caccgaactt aaaaaaacag
    31081 ccttacgaca agatgaccga agtcattatt tttgcgggta gtgatgcctg ggcacatgcg
    31141 aaacagtggc aggaacagga cgggcgactg gctggcgata atgtgcctcc cgttgtgctg
    31201 gctgatgatc aactggatga actggcagac ctgagaatca tcgacgaggg gcgctattgt
    31261 gtccggctgt acaaggcagg ccacatcagg ccatcaaata ttaatgccat tgcgcacaag
    31321 ctggcggcgg cgggtgtaac tgatgcgaat tattaccccg aagggatgca cagccatatg
    31381 cgggagaact ggcgcgaata cctggaacgg gtgcgcggga aagagccggt ggaagaaaaa
    31441 aaccaccagc gaaaaaccac gctaccgatg agcgttggat ctaccggata cgacacgcaa
    31501 ctggattacg tggttaaggg gattattccg gcggtatcgc tatgcagcat atacggggct
    31561 agcgggtcct ataaatcatt ccttgccgga tcgtgggcgt gccatgttgc cactggtcgc
    31621 cagtggggag gccgcagggt tgcacatggt gcggttctct atgtggttgg tgaaggcggt
    31681 atcggcgttc cgcgtcgtgt aaaagcctgg gaggttgtgc acgatgagca ggtgaaaaat
    31741 ctgtatctgg taaaccgccc catctttccg gctgccccgc ttgatgttga tgaaatggtt
    31801 atcgctgccc gtcaggtgga gcgggaaacg ggtaaacctg tacgcatgat tattctggat
    31861 acgctggcgc gttgctttgg tgggaatgat gaaaatgatt cccgtgatat gggggcgttt
    31921 atccgtggtt gtgacgaact gaaacgacgc acaggggcca cggtgctggt ggttcaccat
    31981 tccggcaagg atgagacgaa aggcgcgcgc ggttccagtg catttcgtgc ttcgctggat
    32041 gctgaatacc ggatacgcag ggaggacgca ggaagcgaag cgctggttat ctcatgcacc
    32101 aaaatgaagg acgcggagga actcaaagaa gccgcatatg acttacgcgt ggtggagctt
    32161 tttaccgacg ctgacggtga attaatcacg tcgctggtgg tggtggatga tccgcgccct
    32221 cctgttgaac tggagcgcat cgaggaggca gggaacaaga cggaaaacca taccgcgcta
    32281 tgggggtgca tccgttcacg cacacagaac ggcgacaagt gcacgatccc gctgttacgt
    32341 gatgacatga aaaagctggg gtatgaaatg aaaaacttcc ggcgctggct gtacaagctg
    32401 gaaaaagatg gggttattag tatcgatggg gatgatgtag cgccgctata aaagtgagga
    32461 gcaaaagcga gggggataga aagagggcca aaattagccc gctctccctc acttttcgac
    32521 ctgtatacat cctcaaaagt gaggggtaaa aaatacttat aaaacacaca catagaaaaa
    32581 ccgaaaatcc caactgcgac gaagtgagac gcttgaaaaa gtgaggcgaa aaagtgagag
    32641 gttgcgagaa atgacccaaa aacgcagaga cagaacagag ccaaaatata aagcgttaga
    32701 catgactgag cacaccttaa aggtggcaat cagaacgata gaccgccaca cgcgggaagg
    32761 atacgcgaag gaacatcccg acctgataag cgcattcatg accacggcgg cggcaaactt
    32821 tgccacgctg acagaacggg agattgccga agcggaacag gtaacaacca tcaacgttaa
    32881 aaccggagag gtggaattat gacggcacag atagccgctt acgggcggct ggtggacgac
    32941 ccgcaggtaa aacagaccag caagggcaca ccgatgacgc tggcgcgtat ggcggtatct
    33001 ttgccatgca gccaggcaca ggacggacag gcgacgttat ggttatcggt catcgcattt
    33061 ggcaaacagg ccgacttcct ggctaaacat caaaaaggcg atgttgccag cgtatccggc
    33121 acgatgcagg taagccagtg gaccggacag aacggagaaa cgcggcaggg ctggcaggtt
    33181 atcgcagaca gcgtaatcag tgcccgcgcg gcacgtccag gcgggaacag acgcaaaacc
    33241 acaggcacac agggtaatca gccaccagcg ggaggcgatg acccttacgg tgatgatatt
    33301 tcgttctgag ggggtgacga tggtacatga ccgcatagcg gaggaactgg aggcgaaagg
    33361 cttttaccgg agggcggcgg cgcgatgggg tgaagtcatg cagctggtgg agacagacaa
    33421 ggaacggcat cacatcacga tgcgacggct ggaatgttca aggaaggcac agagggcacc
    33481 ggagccgccg gataatttcg gagacctgaa aaaggcagtc gatcgcactt atgccgaaat
    33541 gggtatagat ggtgctggtg atgaaatatg gcgcaattac caggacagct aatcaaacag
    33601 ccggagaaat ccggcttttt tgcgcccaaa aaaagcccga taaggtcaga gggttcttat
    33661 cgggcttttg catatgaggt ttttggatgc actggcgttc gtgatcggga taatcatttc
    33721 ataatttgca acataactca atattattgc ataaaatgca attctgatta taatcaggac
    33781 tggataaaca tccagttatg atttttttag tcgaagagga atttcttact atggctgaag
    33841 agaaaaaagg cggtgtttcg gtgtacataa gccccgaaat cgtggaggtg ctcaagcagc
    33901 gccacaaaaa aaactatgag gctggcgtgg cggctggact ggatccgctg atgacgccgg
    33961 agccgtcgat aggttcactt gtacgctctt atttacttgc ggcgcttggg atgcataaaa
    34021 attatggggg tgaataatgg caggcaaagc aacggcactt aacactaacc agctttttat
    34081 gtacctgaat cgcggggata ttgcggattt taaattcagc cctctgttta ccacgctgtt
    34141 tttcccgaac gtggcgacat tcagcacgca aaacatcatg ctggataccc tggacattga
    34201 agaagtcacc atgtcggcgt tttgttcgcc tatggtgggt agccaggttc agcgcgataa
    34261 agggtacgaa accagcacaa tcaaacctgg ctacatgaag ccaaagcacg aaatcgatcc
    34321 aacgaaaacc atcatgcgca tggctggaga agatccggca cagcttaacg accctaccta
    34381 tcgccgtatg cgcctgatta ctggcaacat gcgccgccag ataaacgcca ttaaggcacg
    34441 cgtggaatgg ctggcggtga atgcggtaac gaccggaaaa aacatcattg agggcgaagg
    34501 catagaacgc tatgaaatcg actggaagat accggaaaaa aacatcatag agcagtccga
    34561 cggcaaaaaa tggtccgagc atgataaaga tatttacgat ccaatctatg acatcgaact
    34621 atacgcagat caggcaggtt gccccgccaa cgtcatgatt atgggcgctg aggtatggcg
    34681 cacgttacgc agctttaaaa aattccgtga actgtacgat ctttcccgtg gttcagaatc
    34741 cgccgcagaa atggcctgta aaaacctagg cgaagtggtg agctttaaag gctatctggg
    34801 cgatattgct cttattgtct attccggcaa atacactgac agcgacggca ccgaaaaata
    34861 tttccttgag cctgatttgc tggtcctggg caacaccaac aataaagggc tggtggccta
    34921 tggtgcgatt atggatcagg aagcggtaag aacgggcgca acgcaaaaca tgtactaccc
    34981 gaaaaactgg attgaggacg gcgatccggc gattgagtac gtgcagacac acagcgcacc
    35041 gcagccggtt ccggcagata ttcgcaaatt tgttaccgtc aaaattggtt aacgggggat
    35101 tatatggaca ctccatatat tgagttattt gcaggcagtc agcaggtatc aacgacactg
    35161 gtacattttg ccgctgatgc tggcgttatt caggaattta ccccgctgat gctggtggac
    35221 aatggcgagt ttaagccgtg ggatggtcag gaatctggca aggctgttta tctgacttcg
    35281 tacctcgtgg acacgtcaaa gcagaaatca gcacagtgtt acaagacggg gatattcaat
    35341 attgccgccg ttaactggcc tgagagcgtc gacaccgatg cgaaaaaatg cgccgccttt
    35401 gcgggttctg gcgtatccgt tcagccgctg gcccgataag cagggggaac gatggcaacg
    35461 aatgaaagca tcatggcgct accgctggcg agtaaattta aagccgaagc gcgggcaatg
    35521 gctgacagag gtttatcaac ctacgaggcc gtatatcaac tcaacaaact ggaagagcag
    35581 gacaagccgc gcgctgatgc gattatggcg cttcatgagc ataaagacta tcagccgctg
    35641 ttgcgtgcaa tggcaaacgt gccttgtatc gatgttgata cggctaaaaa catcctgaac
    35701 atgaccatag agcaggaacg cccgaaggtt gcaccagagc ttaccgcagc ctttgaaaac
    35761 tttatggaca tgcacagccc acaaaccgta tcagctggca tggcatacga tggcagaaac
    35821 ccaggcgatg acggcgacat cgatcgcata ctgaaaacca tctgagacaa ggccggagaa
    35881 atccggcttt tttgcgggtc cttcctggaa ttatggcccg ttacggggcg gcgacctcgc
    35941 gggttttcgc tatttatgac gtttttccgt gaaggtgaca ccaccaccac ttgattaata
    36001 tttaaccatg cagttaaggt aacattatga ttgataaagc ttgttttgta agtcagcagg
    36061 aaatagctga acatttcaag gttaacagaa ccactattcg cgcatggacc aaacagggga
    36121 tgccgtatct taatgcggat cgcggaaagt ctggcggtta tcacatcggg catacattgc
    36181 tttggtcttc aggtaaaagc cgtcttgagg ccatcagata tcacgtagaa accagtgcgc
    36241 tggaaaaaat tatgtttgcc aggctgcttt catctgagcg tgacgagtac tccagtgaag
    36301 aaacagaaca tcgatttgat gaaggtttgc agatttacgg ctattcaccg gaagatgtga
    36361 gcaaggcacg aaataaaatg gctggctttc tggctgggtg gcgtcatgcc gtaagcgttc
    36421 gccgtgccag catggagcaa tcagccgata cagaacagta aagccgataa cttgttaatc
    36481 actcgcagca atgcatgtga tttgtgcaca ctttcggtta actggttcga tgtccggttt
    36541 tagtgtctgt tttttgcgca tgtccggttc atggaaagca tgtttttata tttttcatat
    36601 ggttaacttg tagagaaacc ggacatggat cccgaaaaat tttcataaat agtgaaaacg
    36661 cgcgaggtcg ccgccccgta acgggtccat aattccagga aggacccgac gacaccagac
    36721 aatcagaacg ataggtgcac aatgacagaa gccgaaatac tgggattaat ccgccgtgct
    36781 ggtggaatca gccagcagac tgacgaacag gccacgcagc cggacagcgt gacagccgaa
    36841 aattatgcgc gtgttgttgc tgaggtgatg cgccgtgatg gtatccagct taatgatgtg
    36901 gatatgcgca acatacggat ccgcgttctt gaaatgctgg cctacaatcg ccgcgtggca
    36961 ctgtatcggg aaacggagaa aattacatac cactggaaga agccggagcg gttgcggcgg
    37021 taactggttg atattttcga aattcgccag cggcgaacat cggggaaacc agcctaaccc
    37081 gttgatattt tcgggaacct caaattgagg aggtcggaaa cataacccgt catattctgg
    37141 atgctgataa ttaccacgca gcccattaag gcctaacccg ttgattcttt cggaaacagc
    37201 cattggcgta atccggcgcg gtaacttgct gaacttaaag caaagcgcaa aattgcgctg
    37261 gctggtgggc atgatgccga tatggtgatc cccataacgg aaatatccac cagcacggca
    37321 acggcagaat agccacaaca cagaaaaacc acgaatatgg ggtttttgtt atggcatggg
    37381 catgatgacc gcccctgctc tgaatggtgg aaatcaccac gcagatcatc caccagcttc
    37441 gtttttataa attaaacatc cgggaaaatc cgaccctgaa ttgtgcattt tttcagcaaa
    37501 tcattttagt tatacgtttg gttatacggt ttaaaagttg aatcatatac attattttaa
    37561 atcatgaagt tacacgctca attaaactac tctcgtgcaa aaatcccaag aaaaaaccaa
    37621 cctttacggt tggttttttt atatctgcaa ttaatttgat aaacagaccg tgacacatca
    37681 cagcctgttt attttctgtt atcagaacgt ccagaccact cccgcctgag tattccacgg
    37741 cgattctacc cctgcgccat tgccatagct gactgacaaa tgaccgctta acgtcggggt
    37801 aaacgatgac cttatcccag cctgataaac gccaggggtt ccagccacat cattattaaa
    37861 tttgccatcg tcattaactt tcacatggtt agaatcggcg tattcctgac gtacggcggc
    37921 tttcagccag ggttccagcg tcgtaccatt ttgcaggtcc atgtgatagc ttaccgccgt
    37981 tcccgcttca gcgcgtaata tccgggtatt tcccacatca gcgcgcatgc cgtttgataa
    38041 cgtgtagtcc tgaccatctg tggtaaagcc ggtaaaggcc agatagggtc taacactcca
    38101 caatccgtca acccaacgga acccgctctc aacatgagca cccgcgccgt tactattgta
    38161 atcgccaaac gctgttgccc cattactcat cttgccatgg atggtgttgg caaaacggtc
    38221 aactttcacc accccatcaa cataggcacc gttctgatgc tcccaaccgg cataagcccc
    38281 cagggtatag ctatcgatat taccttttcc gccgcgatca aaaccaatat cagaatgaga
    38341 gtaaccaaag aacaagccgc gaattgtact gctttcttca cgggagaaac ggctatcgat
    38401 accgagcgtc aggcccgtca atgtttgctc aaaaccagct cccgcatcag tggtcacgtt
    38461 gttgcgggtg ttaattgccg aactccacat cgccgtatcg taactaacgc tttttacgct
    38521 accaagacgc tcacgcacgg tgtccagttc tgcatcaaat accagcggtt gtgcggccgc
    38581 catattcagc acatcagtgg ttgaaggggt aatttgcgcg cgattctctg ccaaactcca
    38641 gctatggttg ccattatcca gcaaggtata ttcatacgta ccgatatcaa caacgcctcc
    38701 ggcattgccc aacgtaaatg cagcatcacc gccgcccgtt gttaccagtg taaggctatc
    38761 tcctgctgcc gggctggcac cggtgtccgt cacgaatatt ttgaaatcac ctgttgcctg
    38821 accggtgacg ttgagctgat cgctctgatg attagccata tcggtacgca tataaaaatt
    38881 gccgtttccc gacagggtat tggtggtcaa cgtaatgaaa ttttccgcac ttgccgtcac
    38941 tgatgaacga gtcactggct catccatcat aatcacattt gcgtcagaca tcgcaaggtt
    39001 ggctaagttg aggagggtgt tttggttcgt cgtagtgata tcggcaatga tggcccatac
    39061 gctattttcg agcgaaacgt ccgctttgct ggtatccacg gcaccatgcg ttctaaaaga
    39121 agcgccctca gaaaccacta ccttacccac caacatagcg ggtgcataat ttatttgcgg
    39181 cttcatgacc tcaagaatgc catcattccc tctgactgac acgttaacca ttgtgccagc
    39241 ccagacatta gcgcggccat tgttgataac catattttca gagatggctt tagaggaata
    39301 cgtgatactt ccattctggt ctgatcgacc gagatcatat accgcatttg cctgcacttt
    39361 cgtaccagta tcctttccca gcgattgcat agtggcatgc tcatcaagga tagtatcaat
    39421 ggcctgcgtg tcctccataa caatgagccc ggaaccatca tccagttcat aattttttga
    39481 cacaccatct tttatactaa attggccttt actgtttgta ccactcactt ccagcgcatt
    39541 cgttgagaca attaattttc cgcctgcttt tttatcaacg ccagttgcag tcccgccatc
    39601 cataacctcc agtaagccgc cactatctac agtagtatta taagcgaagt cattttcttc
    39661 aactcgcaaa cttccgccgt tttccaacag catattgtta gcaataccat tcttgatttc
    39721 gaactgaccg agacggtttg ttccgaatgc ctccatgacc cgcgtgcttg ctttaatagc
    39781 accgcctgct ttctgatcta ccgcaatcgc taatcccccc tctttaacct ccagtaatcc
    39841 tcccttttcg atagcggcaa ggacggtaat cccatctttt tcaacgatta agcgcccgcc
    39901 tttaatcgtg ggatcattcg ctgcgccata aacctgtagc acaccattat tgagggtagt
    39961 ctggtttgcc gcgccaccca tttgaacaat ttgataaccg tcatttaata ctgagtattc
    40021 tgcggtacca ttcatttcaa tgtactgata ccctccgtt
//
LOCUS       DAJTSL010000016        39765 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTSL010000016 DAJTSL010000000
VERSION     DAJTSL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.016"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            4..435
                     /locus_tag="OO433_000744"
     CDS             4..435
                     /locus_tag="OO433_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6049323.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFA
                     VQSGPMLLENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="OO433_000745"
     CDS             518..1420
                     /locus_tag="OO433_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCR6049324.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKSGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAEIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1465..3627)
                     /gene="uvrD"
                     /locus_tag="OO433_000746"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1465..3627)
                     /gene="uvrD"
                     /locus_tag="OO433_000746"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6049325.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCLPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3711..4427)
                     /gene="yigB"
                     /locus_tag="OO433_000747"
     CDS             complement(3711..4427)
                     /gene="yigB"
                     /locus_tag="OO433_000747"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6049326.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4427..5323)
                     /gene="xerC"
                     /locus_tag="OO433_000748"
     CDS             complement(4427..5323)
                     /gene="xerC"
                     /locus_tag="OO433_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6049327.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5320..6027)
                     /locus_tag="OO433_000749"
     CDS             complement(5320..6027)
                     /locus_tag="OO433_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6049328.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6024..6848)
                     /gene="dapF"
                     /locus_tag="OO433_000750"
     CDS             complement(6024..6848)
                     /gene="dapF"
                     /locus_tag="OO433_000750"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6049329.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6893..7087)
                     /locus_tag="OO433_000751"
     CDS             complement(6893..7087)
                     /locus_tag="OO433_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6049330.1"
                     /translation="MKNVFKVLTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGMVHPR"
     gene            7550..7870
                     /gene="cyaY"
                     /locus_tag="OO433_000752"
     CDS             7550..7870
                     /gene="cyaY"
                     /locus_tag="OO433_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6049331.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7910..10456)
                     /gene="cyaA"
                     /locus_tag="OO433_000753"
     CDS             complement(7910..10456)
                     /gene="cyaA"
                     /locus_tag="OO433_000753"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6049332.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLCGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10843..11784
                     /gene="hemC"
                     /locus_tag="OO433_000754"
     CDS             10843..11784
                     /gene="hemC"
                     /locus_tag="OO433_000754"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6049333.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11781..12521
                     /gene="hemD"
                     /locus_tag="OO433_000755"
     CDS             11781..12521
                     /gene="hemD"
                     /locus_tag="OO433_000755"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6049334.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            12543..13772
                     /gene="hemX"
                     /locus_tag="OO433_000756"
     CDS             12543..13772
                     /gene="hemX"
                     /locus_tag="OO433_000756"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6049335.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            13775..14971
                     /gene="hemY"
                     /locus_tag="OO433_000757"
     CDS             13775..14971
                     /gene="hemY"
                     /locus_tag="OO433_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6049336.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15650..15811
                     /locus_tag="OO433_000758"
     CDS             15650..15811
                     /locus_tag="OO433_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049337.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15895..17130)
                     /locus_tag="OO433_000759"
     CDS             complement(15895..17130)
                     /locus_tag="OO433_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6049338.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17277..17353)
                     /locus_tag="OO433_000760"
     tRNA            complement(17277..17353)
                     /locus_tag="OO433_000760"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17317..17319),aa:Pro,seq:tgg)
     gene            complement(17396..17482)
                     /locus_tag="OO433_000761"
     tRNA            complement(17396..17482)
                     /locus_tag="OO433_000761"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17446..17448),aa:Leu,seq:cag)
     gene            complement(17503..17578)
                     /locus_tag="OO433_000762"
     tRNA            complement(17503..17578)
                     /locus_tag="OO433_000762"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17543..17545),aa:His,seq:gtg)
     gene            complement(17637..17713)
                     /locus_tag="OO433_000763"
     tRNA            complement(17637..17713)
                     /locus_tag="OO433_000763"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17677..17679),aa:Arg,seq:ccg)
     gene            complement(17816..19201)
                     /locus_tag="OO433_000764"
     CDS             complement(17816..19201)
                     /locus_tag="OO433_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6049339.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAVGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNESGSSGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19392..20132)
                     /gene="rffM"
                     /locus_tag="OO433_000765"
     CDS             complement(19392..20132)
                     /gene="rffM"
                     /locus_tag="OO433_000765"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6049340.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20135..21487)
                     /gene="wzyE"
                     /locus_tag="OO433_000766"
     CDS             complement(20135..21487)
                     /gene="wzyE"
                     /locus_tag="OO433_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6049341.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTREVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21484..22563)
                     /gene="rffT"
                     /locus_tag="OO433_000767"
                     /gene_synonym="wecF"
     CDS             complement(21484..22563)
                     /gene="rffT"
                     /locus_tag="OO433_000767"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6049342.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRVLAIAAGEVA"
     gene            complement(22560..23810)
                     /gene="wzxE"
                     /locus_tag="OO433_000768"
     CDS             complement(22560..23810)
                     /gene="wzxE"
                     /locus_tag="OO433_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6049343.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23812..24942)
                     /gene="rffA"
                     /locus_tag="OO433_000769"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23812..24942)
                     /gene="rffA"
                     /locus_tag="OO433_000769"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6049344.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24947..25621)
                     /gene="rffC"
                     /locus_tag="OO433_000770"
                     /gene_synonym="wecD"
     CDS             complement(24947..25621)
                     /gene="rffC"
                     /locus_tag="OO433_000770"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6049345.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25599..26480)
                     /gene="rfbA"
                     /locus_tag="OO433_000771"
     CDS             complement(25599..26480)
                     /gene="rfbA"
                     /locus_tag="OO433_000771"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6049346.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLVQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDQERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26499..27566)
                     /gene="rffG"
                     /locus_tag="OO433_000772"
     CDS             complement(26499..27566)
                     /gene="rffG"
                     /locus_tag="OO433_000772"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6049347.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSDRFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27566..28828)
                     /gene="wecC"
                     /locus_tag="OO433_000773"
     CDS             complement(27566..28828)
                     /gene="wecC"
                     /locus_tag="OO433_000773"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6049348.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28825..29955)
                     /gene="wecB"
                     /locus_tag="OO433_000774"
     CDS             complement(28825..29955)
                     /gene="wecB"
                     /locus_tag="OO433_000774"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6049349.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKAPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELVANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30011..31057)
                     /gene="wzzE"
                     /locus_tag="OO433_000775"
     CDS             complement(30011..31057)
                     /gene="wzzE"
                     /locus_tag="OO433_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6049350.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31069..32172)
                     /gene="wecA"
                     /locus_tag="OO433_000776"
     CDS             complement(31069..32172)
                     /gene="wecA"
                     /locus_tag="OO433_000776"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6049351.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32412..33671)
                     /gene="rho"
                     /locus_tag="OO433_000777"
     CDS             complement(32412..33671)
                     /gene="rho"
                     /locus_tag="OO433_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6049352.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33756..33857)
                     /gene="rhoL"
                     /locus_tag="OO433_000778"
     CDS             complement(33756..33857)
                     /gene="rhoL"
                     /locus_tag="OO433_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6049353.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(33998..34327)
                     /gene="trxA"
                     /locus_tag="OO433_000779"
     CDS             complement(33998..34327)
                     /gene="trxA"
                     /locus_tag="OO433_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6049354.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34458..35723
                     /gene="rhlB"
                     /locus_tag="OO433_000780"
     CDS             34458..35723
                     /gene="rhlB"
                     /locus_tag="OO433_000780"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6049355.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEILEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35857..37341
                     /gene="gppA"
                     /locus_tag="OO433_000781"
     CDS             35857..37341
                     /gene="gppA"
                     /locus_tag="OO433_000781"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6049356.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37388..39409)
                     /gene="rep"
                     /locus_tag="OO433_000782"
     CDS             complement(37388..39409)
                     /gene="rep"
                     /locus_tag="OO433_000782"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6049357.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKYYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9868 a        10805 c        10054 g         9038 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgttg cgggagataa agtcggcatc attcgtctgg atgcctttaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttattag aaaacggcgt aattaatcca
      181 cgcattcacc cgaatgttgc ctcgcgtaaa attcgtaacg gtgtagggat taataaacaa
      241 gggaacgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagttg ctttatcttg atggcacaat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagcggaaag gttaagcatt gtacactcga ggaggccaga caaactggga ctggctacac
      481 agctggaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcctatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggcgt
      661 tgaccataat cttaaaatct ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttat cgcctgtatc agactgccag ttactggccg caaggttcgg tgtttgttag
      781 cgtggtcgat ccgggcgtag gcactgaccg taaatcggta gtgctgaaaa ccaaaagcgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcacaaa ctctgggtat
      901 tgatagcgtc cgggaaatcg acgaaaaagc taaccgcctg aaaggttctg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgtctggctt ccggggcgat
     1021 tacgttcgaa caggtggggc cagagcttcc agcaaaagtt gtcgaacttt cttatcagaa
     1081 agcaaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcagaaatca aactcaacga
     1201 cacgttgtgt gtgaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctga acagcttatt
     1321 gaatgtttcc gtggcgctga atatggataa tttcgcgcag aaacatcagg ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg cggaatgcgg
     1441 tgtggcgcac cgcatcccgc aacgtcacac cgtctccaac cgggcataag ccgccaccag
     1501 ccatttaata ccctggccct ggaatgccac ctgcaaacgg ctatgttcac cgctgccttc
     1561 catattgacg atggtgcctt caccaaactt agcgtggcgt acacgctggc cgagcttgta
     1621 gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctgaccg ggcggcttac
     1681 cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccaa taaagcgcga
     1741 cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg tcagcgtcag
     1801 tttctgcatc gcgcgggtta cgccaacgta ggccagacgg cgttcttctt ccagacgccc
     1861 gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga cgataaacac
     1921 ctgcgggaac tccagacctt tcgccgagtg catggtcatc agttgtaccg catcctgcca
     1981 ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg gataggaacg cctgcagcgg
     2041 cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca ccagttcctc
     2101 taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca tggtacgcag
     2161 gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg cagtttcctg
     2221 cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg ctggcagcac gtccggcgag
     2281 ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc gatcgcgcga
     2341 tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg tattcaccac
     2401 acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg cgcagatacg agagcgcatc
     2461 tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg gcatactcgc
     2521 ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta ctgcggtaga gaatggcgca
     2581 ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg cggttaacca caaaacgcgc
     2641 ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg cgccatcggt
     2701 ccacagtttt ttacccagac gcccgttatt gttttcaatc agggcgttag cggcgctcag
     2761 aatattgctg gtagagcggt agttttgctc cagacgaata gtttcggcac cggggaaatc
     2821 attaaggaaa cgctgaatat tctccacctg cgccccgcgc cagccgtaga ttgactggtc
     2881 gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc agcaggcgga tccacgcgta
     2941 ctgaatgttg ttggtatcct ggaattcgtc caccaggata ttggtaaaac gttcgcggta
     3001 gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca gcagctcggc
     3061 gaagtccacc aggcccgcgc ggtcacacgc ttcctgatac gcctgataca ccttctgcca
     3121 ggtctgctcc accggattac cgtagctttg aatatgatgt ggacgcaggc cttcatcttt
     3181 ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt cgaggttcat
     3241 cgccttgatt aggcgtttga gcaggcgtag ctggtcctcg ctgtcgagga tctgaaaatc
     3301 ctgcggcaga ttggcgtcca tatggtgcgc acgcagcaga cggtgcgcca gcccgtggaa
     3361 ggtgccgacc cacatgccgc cctggctggt gcccattagc tgcccgatac gatgacgcat
     3421 ctccgccgcc gctttgttgg taaacgtcac cgccataatc gagtatggca agcagttttc
     3481 cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac tgcccgcgcc
     3541 cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt tgtcattaag
     3601 gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg ttgggcggag
     3661 aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttgctga ttatatcagc
     3721 gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct gtcccaggtt
     3781 tgcatcagat cgccattttc cggtctgatc caacaggcct gcattccgct gcgaattgcc
     3841 ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat cggcacgttg
     3901 agtttttccg cagccagaaa gtacatatcg ctgaacggtt ttgagcgccc gtgcgggcca
     3961 gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg ggcgttaccg
     4021 ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc gtgagtttgc
     4081 tgcgggacgt cgattcggct gcgccatttg gcaaagttga tcattgctgc gtgtgcgcct
     4141 gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat cgaacgaaaa
     4201 cgccagcgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc ctggcgcagg
     4261 cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg cacaaaggta
     4321 agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt atcatccagg
     4381 tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat ttcccccgtt
     4441 tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca agatgagtat
     4501 agatttgcgt ggtggagagg ttggcatgac ccagtagctc ctgcacacca cgaagatcgc
     4561 cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa tttatgtggg tgaacgtgat
     4621 tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta cgcgcggaga
     4681 tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac aggtcgcgca
     4741 aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg cggcgctctt
     4801 tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt ttgatatcca
     4861 gccccaccag ctcggaaaga cgcagaccag cgccgtacat cacttccagc attgcacggt
     4921 cgcgtacagc gaggggatcg ttgatatcaa tatccagcag ccgattcata tcgtcgacgt
     4981 cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc gccgggttag
     5041 ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca gaaagccgta
     5101 acgccagact tgctgctccc agccctttac ggcgactgcg tacagcaaaa ttgcgcacca
     5161 tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa ttgatgatcg
     5221 cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc tccacgctca
     5281 gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt caatccaacg
     5341 ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc cttgcccttg
     5401 ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag catcgcttcc
     5461 cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca ccagcagctc
     5521 tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa tacgcagcgg
     5581 ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac ctaagcgcca
     5641 gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg cccagcggtg
     5701 aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct gcaggtagag
     5761 taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg ccatgttctc
     5821 ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca acgaaacggt
     5881 gccgcgtacc ggatgtggca cacgtatcgc ttctactgcg cgcgcattac ggataaaaaa
     5941 ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg tgagtgtttc
     6001 ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac atgtaccgcc
     6061 gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag acgaccgccg
     6121 gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac tgcaaccgcc
     6181 gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata aacgcgtaaa
     6241 cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc cggaaaacgc
     6301 tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac atcatcgacc
     6361 tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt ctgctcggcg
     6421 gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacagc ggaaggttcg
     6481 aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt cagaaccatc
     6541 cgcccgttgg cggtgctgac gcggatatcg cgcttattgg tcagcccttt caggcgcaca
     6601 aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc atcagcattg
     6661 aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac cagcagctgg
     6721 tcaaacccta cccccaggtg ccgatccgcc agccgacgaa tcagctccgg tgaaaaaaag
     6781 acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg cattttcgag
     6841 aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta attcacctgg
     6901 gatggaccat cccagtggcg cgatcgtttt tatccggcac cgtggattgc gtttgcgtct
     6961 ctaccggttt ggtcggcggc ggtgcatttt tatctgcagg cgggaaatag agcggacctt
     7021 tcagaccgca gcccgtcagg ctgaagagag taagtaatac agtgagtacc ttaaacacgt
     7081 ttttcattat ggattgcctg taaagttcat cgctttctgc tttctatcat cgcaggagaa
     7141 ggcgtaaaag caagcgttta gcgactcttt atgaagtctg aaaagtgagg aaatgctgcg
     7201 agtataacgc ttactcgtga ggatttatgt tctgctggca gtttcgtgat cggcacctcg
     7261 cgccaggatg gctttatgca ctatcgcaat gtggtttgtt tgttggcttt tttagttccg
     7321 tcagcgtgag gatgctcgct cggtaaacct gatcataatg tttaccagaa acagggaaag
     7381 gcgtggtgta actgcgccct aatgaagaga caaatctttc gccacacaga gacatcatta
     7441 ttccggtgac ggatgaatca tgccggaacg cagcagcaga ggtgcaaact ttgaagccct
     7501 gaccccatgc cgctatactg ccgccatcac ttaaaaacag gatacaacaa tgaacgacag
     7561 tgaatttcat cgcctggctg atcaactgtg gctgaccatt gaagagcatc tggacgactg
     7621 ggatggcgac agcgatatcg actgcgaaat caacggcggc gtgctgacca ttacctttga
     7681 aaacggcagc aaaatcatta tcaaccgcca ggaaccgctg caccaggtat ggctggcaac
     7741 caaacaaggc ggctaccatt ttgatctgaa aggcgatgag tggatttgcg atcgcagcgg
     7801 cgaaaccttc tgggatttgc tggaacaggc ggcgacgcag caggcgggtg aaacagtcag
     7861 tttccgctaa gattgcatgc cggataagcc tcgctttccg gcacgttcat cacgaaaaat
     7921 attgctgtaa tagcggcgta tcgtgatcct gattggcagg cggcatgtta ccgatagatt
     7981 ttgtgcggaa cggaatcacc tgttcacgac catcaacctt cacaatctga tagaactgcg
     8041 gcaggttgaa gttgatgaag cttgagccgt aggtgaagcg gtcgtgcgat gacgagtaga
     8101 agcgactgac gtcacgcacc agttcctctt tgctgccttc gcagtggtga tacacctcaa
     8161 cccggttgct ttcgtcaaga atgtagatat taaagccatt ctcgtcttgc gtttcttcga
     8221 agaagaactg gatgatccct tcactagcaa agccgtccac caccgccggt aatttgacgt
     8281 gattggtttc aacctgcact gacaggccgt gcagtttgtt atgcgaaata gcgccataaa
     8341 actcgatggc gttctccagc ttctgtaccg atacattcag gcgttcgaag aacaagcccc
     8401 aggtttgacc agaaacgcgc agcgccttga aacgcccggt ttcctggcgg gtgctggaaa
     8461 ggcgcagttc aatacactca gaaaccagtt gctgcacgcg agtacggatt aacccgcaca
     8521 gatgctggct ataacagaag acttccacgc tatctggcgg tgcggcgtcc tgatgcattt
     8581 tgccgagaat agttttcagg gcttcgatca tcgattgctc gccgttgaag tgcagcgtac
     8641 gcacttcgtt ccacgagttg cggtacagca ggtcaacgct acctaccagg caattttgat
     8701 tctcgccaaa gctgaagaca tccagcttac ggaaatcaaa atgcaccacc tgattgcgga
     8761 acgccgctgt cgggtcatat tccaggttaa cgataatcgc cagatggcgg atctcacacg
     8821 ggctgtagag cgccttcggt gtcggtgcag gtaagcgcag cgggaaatgg tgcgacacgt
     8881 cggcgaccat ctcctgcaac ttaggcaaat cgacaatgcc gttaccttta atatacaaac
     8941 gggtgcgcga ggtcagcagg ccgttaaacc atgcccacgc caccagtttg ttcaggtaac
     9001 ggttatattc cagcggctga tggctgatga tcgactcaat atttggcgcg cggttataca
     9061 gataccaacc tgaacggtta gcacggcccg gcggcacata aataaaggtc agattcggtt
     9121 ccgagagatc gggtgaaatc tgcgggttta ccagcgtcac tttacctggt aatgcttcaa
     9181 acgcggcata cagcttacgg gtcagcacgc cgatatcctg cggactggcg gagacgctaa
     9241 ggttattgcg acgcgcaaag cggatcagat tacggtagct ctgcatcatc gcgtcgagca
     9301 actcgttgtg cgcctcacgc acctgatcaa tcttccagtt agcgcggtta tcgagcattg
     9361 ccagacgagc ttcgtcccaa ccccactcgc tcactaactg gctcaacact gcgcgacgcc
     9421 agcctacgca ggcgcgttca cggctgagct tttcgcacac ttttaaatag aagcagcgac
     9481 gtactaaatc cagacgggta aaatcttcaa tcgccgtcag gtattcagta acacgctcca
     9541 gcatcatgca gtatggatcg agaccaaacg atacaatctc accgtcgtgc aaacgctgtt
     9601 tgatatcttt cgccagcaga cgtgggttcg ggtattccca ggaataggct tccagcagca
     9661 gtgttttcag taccgctttg tatggggaat cgatactctt gtagagctgc caaaggctgg
     9721 caccaaagta ctcttcagca gaaagcgagc ttaagccacc gagatccagc cattcatttg
     9781 gcgtcagcac gccctgcgcg taaagcgtca tcacatagtc gtcgtaatgc tcttcttcgt
     9841 cgcacggcac catattccac agaatacgct taccggcgag acgcacggcg gtacgataaa
     9901 attcgtcaag cagcagtata tgctgggtgg agccacaatc ttcgcccccc aagctgccgc
     9961 tttcattatg acggaagcgg ttttcatcaa tcaggaagaa gctgacttcc acacccagcg
    10021 aggcggccca gctttccagc agactacatt tacgttgtag taactggcgc tcttcgctat
    10081 cgagccagga ttgatgacag acccagatat ccaggtcaga ggaacaactt tgccctacgg
    10141 acgaggtgct gcccatggtg tatacaccag taattggaag ctcacctttc ggtggatcct
    10201 gtactgacat tccacgatac agttcaagct cgttcaggta gtggcgttga gtttcatcag
    10261 gcgtgtaaag gcaaatgcct ttgggaacgt taccatcaag gtaacccggc attagcggat
    10321 ggtgatagtg caacaatgtc ggcagtagac tgtagacctg ttggaatgca ggccccatag
    10381 cagcaagcgc gcgatccaca cgcaattgat ttatggcatc cagtctctgt ttcagagtct
    10441 caatatagag gtacaagacg tatcgcctga tttgctaccc gtcatgactg tgattccgcc
    10501 aacatcaacg gtaacacgcg gcattcggga tatttcgtat gtcaaaggta accgttacca
    10561 cttttcgcgc ctggtttttt tagtttcacg acgaaaaaat ggtctaaaac gtgatcaatt
    10621 taacaccttg ctgattgacc gtaaagaaag atgcgctaca tacaagtgta gcaccgttta
    10681 ttctctgtaa attccttatt acgacggcgt gaaacgcctg tcaggatcca ctgccagacc
    10741 tcattttacg gtttgcgcag gcgtctacgt ttcaccacaa cactgacatc actctggcaa
    10801 ggatgttagg atggaccacg gatgataatg acggtaacaa gcatgttaga caatgtttta
    10861 agaattgcca cacgccaaag cccacttgca ctctggcagg cacactatgt caaagacaag
    10921 ttgatggcga gccatccggg cctggtcgtt gaactggtac cgatggtgac gcgcggcgat
    10981 gtgattcttg atacgccgct ggcgaaagta ggcggaaaag gcttatttgt aaaagagctg
    11041 gaagtcgcgc tcctcgaaaa tcgcgccgat atcgccgtac attcaatgaa agatgtgccg
    11101 gttgaattcc cgcaaggtct gggactggtc actatttgtg agcgtgaaga tcctcgcgat
    11161 gcctttgtgt ccaataacta tgacagtctg gatgcgttac cagcaggcag tatcgtcggg
    11221 acgtccagtt tacgtcgcca gtgccaactg gctgaacgcc gcccggatct gattatccgc
    11281 tccctgcgcg gcaacgtcgg cactcgcctg agcaaactag ataacggcga atacgatgcc
    11341 atcattcttg ccgtagccgg actaaaacgt ttaggtctgg agtcccgcat tcgcgccgcg
    11401 ttaccacccg agatttctct tccagcggta ggacaaggtg cggtcggtat tgaatgccgc
    11461 cttgatgatg cacgcactcg cgagctgctt gccgcgctga atcaccacga aactgcactg
    11521 cgcgttaccg cagaacgcgc catgaatacc cgtctcgaag gcggatgtca ggtgccaatt
    11581 ggtagctacg ccgagcttat tgatggtgaa atctggctgc gtgcgctggt cggcgcgccg
    11641 gacggttcgc agattattcg cggtgaacgc cgcggtgcgc cgcaagatgc cgaacaaatg
    11701 gggatttcgc tggcagaaga gctactgaat aacggcgcgc gcgagatcct cgctgaagtc
    11761 tataacggag acgccccggc atgagtatcc tggtcacccg cccgtctccc gctggagaag
    11821 agttagtgag ccgtctgcgc acactggggc aggtggcctg gcattttccg ctgattgagt
    11881 tttctccggg tcgacaatta ccgcaacttg ctgatcaact ggcggcgctg ggggagagcg
    11941 atctgttgtt tgccctctcg caacacgcgg ttgcttttgc ccaatcacag ctgcatcagc
    12001 aagatcgtaa atggccccga ctacctgatt atttcgccat tggacgcacc accgcactgg
    12061 cgctacatac cgtaagcgga cagaagattc tctacccgca ggatcgggaa atcagcgaag
    12121 tcttgctaca attacctgaa ttacaaaata ttgcgggcaa acgtgcgctg atattacgtg
    12181 gcaatggcgg tcgtgagcta attggggata ccctgacagc gcgcggtgct gaggtcactt
    12241 tttgtgaatg ttatcaacga tgcgcaatcc attacgatgg tgcagaagaa gcgatgcgct
    12301 ggcaatcccg cgaggtgacg atggtcgttg ttaccagcgg tgaaatgttg cagcaactct
    12361 ggtcgctgat cccacaatgg tatcgtgagc actggttact acgctgtcga ctattggtcg
    12421 tcagtgagcg tttggcgaaa ctcgcccggg aactgggctg gcaagacatt aaggtcgccg
    12481 ataacgctga caacgatgcg cttttacggg cattacaata actctcataa caggaagcca
    12541 taatgacgga acaagaaaaa acctccgccg tggttgaaga gaccagggag gccgtggaca
    12601 ccacgtcaca acctgtcgca acagaaaaaa agagtaagaa caataccgca ttgattctca
    12661 gcgcggtggc tatcgctatt gctctggcgg cgggcgtcgg tttgtatggc tggggtaaac
    12721 aacaggccgt caatcagacc gccaccagcg atgccctggc taaccaactg acggcattgc
    12781 aaaaagccca ggagagccaa aaagccgagc tggaaggcat tattaagcaa caagctgcac
    12841 agcttaagca ggcgaatcgt cagcaagaaa cgctggcaaa acagttggat gaagtccaac
    12901 aaaaggtcgc caccatttcc ggcagcgatg ctaaaacctg gctgctggct caggccgatt
    12961 ttctggtgaa actcgccgga cggaagctgt ggagcgatca ggacgtcacg accgctgcag
    13021 cgttgctgaa aagtgcagat gccagcctgg cggatatgaa tgacccgagt ctgattaccg
    13081 ttcgtcgggc aattaccgat gatatcgcca gcctttctgc agtttcgcag gtggattatg
    13141 acggcatcat ccttaagctt aatcagcttt caaatcaggt agataacctg cgtctggccg
    13201 ataatgacag cgatggttcg ccgatggatt ccgatggtga agagctttcc agttccatca
    13261 gcgaatggcg tatcaatctg caaaaaagct ggcagaactt tatggacaac ttcattacga
    13321 ttcgccgtcg tgatgacacc gccgtaccgc tgttagcgcc aaatcaggat atctatctgc
    13381 gcgaaaatat tcgctctcgc ctgctggtcg cagcacaagc tgtaccgcgt caccaggaag
    13441 agacttatcg ccaggcgctg gagaacgtct ccacctgggt acgtgcttac tacgatactg
    13501 atgatgccac caccaaagcg ttcctcgacg aggtggacca gttaagccag caaaatatct
    13561 cgatggatct tccggaaacc ctgcaaagcc aggcgatgct ggaaaaactg atgcagactc
    13621 gcgtgcgtaa cctgctggca caaccggcag cagggacaac ggaagctaaa cctgcacctg
    13681 cacctgcacc tgcacctgca cctgcacctg cacctgcacc tgcacctgca ccgcaaggtg
    13741 atactccggc agccgcgccg caaggagaat aaccatgcta aaagtgttat tactctttgt
    13801 tttgctgatt gcggggatag tggttggccc gatgattgcc ggtcatcagg gttatgtgct
    13861 gatccagacc gacaactaca atatcgaaac cagcgtcacg ggcctggcga tcatattgat
    13921 tctggcgatg gtagtgctgt ttgccattga gtggctactg cggcggatct tccgcactgg
    13981 cgcgcacacc cgtgggtggt ttgtcgggcg taagcgtcgc cgtgcacgta agcagaccga
    14041 acaggcgctg ctgaaactgg cggaaggcga ttatcagcaa gttgaaaagc tgatggcgaa
    14101 aaatgccgat cacgctgaac aaccggtggt gaactatcta ctggctgccg aagccgcgca
    14161 acaacgtggt gatgaagcgc gcgccaacca acatctggaa cgcgcagcgg agctggccgg
    14221 caatgacacc attccggtag aaatcacccg tgtacgtctg caactggccc gtaatgaaaa
    14281 ccatgctgca cgccacggcg tggataagct gctggaagtt acgccacgcc atccggaagt
    14341 attacgtctg gcggaacagg cgtatatccg cacaggtgca tggagttctc tgctggatat
    14401 catcccatca atggcgaaag cccatgttgg tgatgaagaa catcgtgcaa tgctggaaca
    14461 acaggcatgg attggcctga tggatcaggc gcgtgccgat aacggtagtg aaggtttgcg
    14521 taactggtgg aaaaaccaaa gccggaaaac gcgtcatcaa gtagcgttgc aggtggcaat
    14581 ggcggaacat cttattgaat gtgacgatca tgatactgcc cagcaaatta tcatcgatgg
    14641 cctgaaacgc cagtacgacg atcgcctact gctgccgatt cctcgactga aaaccaacaa
    14701 tccggaacag ctggaaaaag tgctgcgcca gcagatcaaa aacgtcggcg atcgcccgct
    14761 gttgtggagc acactgggcc agtcactgat gaagcacgga gaatggcagg aagcatcgct
    14821 cgccttccgc gcagcgctga aacaacgtcc ggacgcctac gattacgcat ggcttgccga
    14881 cgcgctggac agactgcaca agccggaaga agctgcagcg atgcgtcgcg acggtttgat
    14941 gttaacgttg cagaacaacc caccacagta gttccttctc acccggaggc aagcacctcc
    15001 gggacctttc tgatgcataa aaaaacgcct gctcttatta cggagcaggc gttaaaacag
    15061 gtctgtatga caacaagtgg gtgcttcact caacgttgtg tccatggtgt ctgatgaggc
    15121 ataagcgaca tctgtcagtg gacgataagc accgtaaacg gctctgcgtc attccggagt
    15181 ttatgaggca ctaaggcgaa cataagagat ggaatgagca tctactcgtt tattatgcca
    15241 cagagaatcg ggaaataaca tcccttaaca cttgttatga gataattctg taatcctctt
    15301 tgcttcctga gtaataactt cctgagtgaa tatttaacct gagcttgatc ctacacatac
    15361 ttattatgaa tgataaaatt cattcaatta ataacacata tattaattgc cgttaaaact
    15421 aaaaaaagca tcaataatca acgcgatata ataaacctgc cttacatatc aactgcgcca
    15481 gaggtaggat tgaaaacgct ctcctgattt tccaattcat tttctggata aataaataat
    15541 ttatttttgt cactattatt tatgtaatca tcctgtcagg gagagggatc tcaattatca
    15601 atgcttaatt acgtcatcat tttgatgaca tgaaaccgga gcattttgca tggaattttc
    15661 gttttcaccc aaacttcttg ttgttgctgt cgccgccgct cttcctctca tggccagcgc
    15721 agcagatacc ccgtcaactg ccaccgcacg caaaggcttt gccggatacg atcacccaaa
    15781 ccagtatcca cagaacgctg caatttattg aatttgcacg atcatgtagg ccggataagg
    15841 cgtttacgcc gcatccggca atcaaccgca ggcggccaca aaatggccgc tattttactt
    15901 attcaccacc agcaaatgtg cctgcataat gtcactggcc gggcgaccgt gcgccagcaa
    15961 atcagccatt gctttaagat atggcggtag atggcggaaa taacgctgat acccggcaca
    16021 caaataattc agccccggtt tgccgctggc atcgagcatg aagcggtgtt tcgggcagcc
    16081 tccccagcac gcttttaaca cgttacaact gcgacactgc gccggtaatt gcttaaattt
    16141 atcttcacca aacacctgct gttgcgggga atcgatcatt tctgcaattg tttgctgatg
    16201 catattcccc agccgatatt gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt
    16261 gtgctcaaca atcaccgagc gcccacaggt tggctgatga tggcaaaccg cacccggcgc
    16321 accgacaaaa ttggcaaacg cccattcgat attcatcacg aaaatcttgc cgacgtcgcg
    16381 tttgatccag tggtcaaaaa tcgccaccag aaattcacca aattcatcgg ggcgcacaga
    16441 ccattccgtc agttccccct gaatatcacc aggcgcatgt agtttcagtc catcgctggc
    16501 agctgtttca tcagctaggc gctcgaccac cggaataaac tggatgaatt cgactcccgc
    16561 atcgcacaaa aaatcataaa cctgcaacgg ttgctgcgcg ctggtgcggt tgacgcagac
    16621 cagcacgtta tagtcgacat gatgtttttg cagcagcgtc agggcacgca tcaccagctt
    16681 atgcgttggt ctgccacctt tggtcacgcg atattgattg tggatctcag ccggaccgtc
    16741 cagcgataac ccaacaagaa aatgattttc tgccagaaat gcacaccatt tatcatcgag
    16801 cagcacgccg ttagtctgga agctgttact tatcttcctg ccagcaccgt atttcgcctg
    16861 taactttacg gcacggcggt aaaaatccag cccgagtaac gttggttcgc cgccctgcca
    16921 ggtaaaagcc acttcgtttt gcggttcgct ggcagcgata tagtgacgga catacgcttc
    16981 cagcgtgtca tcgtccatat gcgtgactgg cttttcgcgg taaagggatt gtttttcgag
    17041 ataaaaacag tagtcacagt tcagattaca atcggaacca ctcggtttcg ccatcacatg
    17101 aaaagcacgc gttggaacct gttgcagcat ggtcactcct tgcctgaagt catccttcaa
    17161 tacgttaagg gcgaaatcat accgtggggc aatgagttat tacgggcgtt tattgcgaag
    17221 agagggtgac gaaatgcaca gaaaacaaaa aaccccgccg aagcggggtt caaaattggt
    17281 cggcgagaga ggattcgaac ctccgaccca ctggtcccaa accagttgcg ctaccaagct
    17341 gcgctactcg ccgaaatact gctttttgaa tttttagttc aattatttaa agtcgtggtg
    17401 cgaggggggg gacttgaacc cccacgtccg taaggacact aacacctgaa gctagcgcgt
    17461 ctaccaattc cgccaccttc gcattgtcac aacttctaat aatggggtgg ctaatgggat
    17521 tcgaacccac gacaactgga atcacaatcc agggctctac caactgagct atagccacca
    17581 ctacaaatct tgttacgcgg tattactacc accgcagctc aagcgccggg actaaatggc
    17641 gcgcccgaca ggattcgaac ctgagacctc tgcctccgga gggcagcgct ctatccagct
    17701 gagctacggg cgcttagcgc cgttgcgggg gtggataata cggacttccc acccctctgt
    17761 ctagtccctt tttaaataaa ttgcgcgttt ggttacggtt tgtgcgtttt gctgcttatt
    17821 cctccagttt atgcgctttc ccgtggcgat taaggccaaa aactttataa atcgccgtca
    17881 ccgccagcat aaagatgatg ccaacaaaca gcgacatacg cgtatcttca ttaaagtaca
    17941 tgccgatcaa aacgcaaatc aggaatgcca ttgttacgta gttggcccac gggaacagga
    18001 ttgagcggaa cggatggctg gcaatcgccg ctttatgtgc acgccgaaaa cgcagctggc
    18061 ttatcaatat cacaaaccat ggcaccatcc ccggaagcac gctggcactg tagacgtaga
    18121 caaacacacg ctgcggattg ggaatgatgt agttcaggca tgagccaatt agcagaatag
    18181 caatagatac tgccacaccc gcaactggta cgccgtgacg ggaaactttc gccatcgccg
    18241 ccggtaactg acggtttttc gccagtgcgt agagcatacg tccgcaactg tacatgccgc
    18301 tgttacagcc agagagcgca gccgtcagca ccacaaagtt gataatgccc gccgctgcgg
    18361 taataccgat tttggcaaaa gtcagtacga acgggctgcc gctgctgcca ctttcattcc
    18421 acgggaagat ggtgacgata acgaaaatcg cgcctacgta gaaaatcagg atccgccaca
    18481 gcactttgcc tacggcactg cgcaacgtca cctgcggatt cttcgcttca ccggcagtaa
    18541 tgccaatcag ctccacgccc tggtaggacg ccaccacaat acacagcgcg gtcaggaacc
    18601 ctttccagcc acccgcaaag aaaccgccat gctctgtgag attgctaaaa ccaatcgact
    18661 gcccgccatt gccaaagcca aagaaaatca cgcccaggcc aatgacaatc atcacgataa
    18721 tcgtggtgac tttgatcatc gcgaaccaga actcgatttc gccgtacaac cgcaccgccg
    18781 ccagattcgc caacgccacc agcgccactg cgatcaatgc gggtatccac tgcgccatct
    18841 ccgggaacca gaactggaca taaacgccaa cggcggtgat ttctgagatc cccaccgcca
    18901 tccacataaa ccaataagac caggcggtga gataaccaaa gaacgggctc atataacgat
    18961 gcgcataaac ggcgaacgaa ccggtaaccg gttcgaggaa caacatttcg cccattgaac
    19021 gcatgatgaa aaagacgaat agcccggcga tgatataggc caacaatacg gatggcccgg
    19081 cccatttcaa ggtactggcg gcccccataa acaggccgac gccaatggtg ccccccaggg
    19141 cgatgagttc gatatgtcga gcttccagcc cacgctgtag ctctggtttg ttatctgcca
    19201 taaatcctcg tgttgtgttt gcatgctttc cggtgttacc ggttatcgtt atgggtacat
    19261 cgagtgttgc gaatgttttc gtaattcagg agaaatggca aataaagcat taaaaatttg
    19321 aatgctttgt gtaataaaaa agcagacagg cgacggagtg accactccgt cgctttacaa
    19381 agagaggaaa atcagaggtt gccggtgtag tgccagcgta aataacgcag caaacgaagc
    19441 tgacgcttaa tgcggctcgg ctgcgaaagc aggcggtaga gccactccag tcccagcgtt
    19501 tgccagattt tcggtgcgcg ttttacgtga ccggtgaaaa catcgtaagt cccgccaacg
    19561 cccatataca gcgcatctgg atgcaccaga cggcagtcgc gcatgaagat ctcctgcttt
    19621 ggcgatccca tcgcaacggt gacgatttgc gctccgctgg catgaatgcg ttcaaacagc
    19681 gcctgacgct gctcgggttt aaaataacca tcctgactgc caacgatatt cacattccac
    19741 tggttgcgca gtttagcttc agtttgcgcc agcacttcag gtttaccgcc cacaagaaat
    19801 accggcgtcc cttctttgcc tgcgcgcgcc atcagctctt cccagagatc ggcaccggca
    19861 acgcgggaaa cctgcgcctg cgggtacttt ttacgtactg aacgtacaac gctgatgcca
    19921 tccgcatatt taaattcggc agcgttaatt aactccctga cctcggcgtt atcttcaatg
    19981 gtcagcattt tttcagcatt aatggcaacc agcgttccct gcttaagctg cccgtcagca
    20041 aacagataat cgagggcgtg ctgcatatca cgccaaccaa tcaactgtaa gccacgcagc
    20101 gtataggtcg gtgccgtggt gttgttattc attgttatcc ttcaacctgc gtccggagcg
    20161 atgattttgt acgtttatga atgagtccgg cgctttcaaa aagccagtac aacagttttg
    20221 cgatcatcag acatgcgccg aagaccacga taaaaaagac tacgcgtgag acaaacgaat
    20281 ccagcccttc acgtgccagc acgatcatat tgaagatcgc cccaaagcag aaactgtgca
    20341 atatcgcagc cttatagcga ttaggctcgc ggttgcccag ctcatacagc cagtcgaacc
    20401 atttgatgat cagaccaacc acgatcgccc cgagcgggat gaacaacgcg ccgcccatca
    20461 ccaccagtga gcctataagc gtaggcgaga tcgccagtcc ggagtggtta ttcagcactt
    20521 ccctggtaaa gtagttggct gagttcagca ccatactcgg gcgacccggc cacagccagg
    20581 aagggataaa gacatagaaa tcgcggacaa ttggagccag gccctggaag tcgatgttgt
    20641 cgtagttctg caacagcaac gccagattct cccacggcga gaaggtgtcg cgagtgagat
    20701 agagaaacgt atagaacgct tcatcgccgc tcacattcat tccatagcgt tttagcgcca
    20761 gccagaacat gcccacaatc cccagcacgc ccgccgccgc cagcatccac aacgaaatcc
    20821 agccgcgaat aatgccaata aacaggaaga tagcgaatgc gatgatgata ttggcgcgag
    20881 tgccgccgac aatcatataa gtcagcaagc caaaggcgac cgtgctgacg aggaaaaaca
    20941 gccacgcttt gctatcctgg cgcagaaagt agaccaccag catcgccggg atgaaaaagt
    21001 aaaagaagcg ttttaacgcc acgccggaga cttcactgga gaagatctga ctgtaggagt
    21061 taagccggaa cagcaaaaag ccgttgtgca tgaagaagat gccgacgctt accagcgcga
    21121 tgcccatcag gatcacccac gtaagattgg tctccacgcg gttcatggta aacagcggac
    21181 ggcgcggtac atcagcaacg cgtttgcgta ggcgggtttt gtaggtgaca tagtaaaccg
    21241 cgtagaagca gcccgcagaa agcaacgcct gcaacaagat ttctggcggc gcgacaccaa
    21301 catcaaagcg aaataccagc acgctggtca gcgggaagcc gaagaaaaag gtgagcaaaa
    21361 acagcaatga aaagaagaca ttgaagttaa agcgcacacg gcgaaactca aaccaagtca
    21421 gcgtggcaat aaacagcgtg cagagcagcc agaccacaaa caggccactg aattgcagca
    21481 gactcatgcg acctccccgg cggcaatcgc caacacccgc tgccagcctt gtagatagtt
    21541 agggctaaag aaggcaatgg tgtttttatc caccgacgcc aactggcgct gcgcttcacg
    21601 cacaatatcc tcgttgagat cgtcggtagt aaacagcacc ggcaaatgct gttccgtcat
    21661 atcctgccag aacggatttt cccggttaag cacacaagga atgcccgcct gaatcagtaa
    21721 gcaaagcgta ccaatgccct gctggcgggc aaaaataaag taaccaagat cgcactgacg
    21781 aagtagcgcc agataggcgt caaattccag tttttcgctc aaaatttgca gattttcttc
    21841 gctgaataac tccagccccg cctgacgaac ttcctcaatg tacgcttcgt tattaggcgg
    21901 atatcccatc ggcaccacca cttttaccgt atcgccaaat tgctgatgaa cggcgcgcaa
    21961 ggcagcaata tggtcattgc tgcggtcacc ggagttcccc accagaatgg tcattttccc
    22021 ttcacgttgc cgatcgttcg ccatcgtatt gagagaaggg tccatccgcg tcgggaagta
    22081 cagcagttcg ccccgcacct ttggatgcgt tttggcaaaa aagctcaaat caccgcgggt
    22141 ggcaaataca cagccgactc gcttttgcgc caggcgacgt agcgggtaaa aaagcttata
    22201 tctcaagcca ctggaaagct catacaggtc tgccccccag atatgccaga aaaactggct
    22261 gggcttaatg ccaccactca gcagagccag ccacagtgtg ggattgaact gaccgtggaa
    22321 aaaaaatcgc tgctgacggt tagcttttgc tttcgcgatg accgcttccg ccagcgattt
    22381 tttcccaggg aaaaattgca cagaaagcgc cggacagcta tcacttaagc cgtcgtcctt
    22441 gccaacaacc ataaactcgc gcgcgtgctc gctcgtcgcg gccagcgcgt cattgaaaaa
    22501 ccgcaaaacg gttcggttat ggtgagggat atccgatccc agtacgtgaa tcagtacagt
    22561 catgcccgcc tacgccagag taaaaacacg ccacaacaaa gagaaaaata gacgatataa
    22621 gttgccatat atgcctgcgc cgcgcccagt gcaccatgcg cagggattag ccagtgggca
    22681 aataccatca ataaagtgaa ctggctgact tccgccagaa tataaaaccg cagtgacgct
    22741 ttggcgatca ccagataacc aaagacataa gcgcccactt ttagcacatc acccactaac
    22801 tgccaggcaa agagatcgcg catagcggta aatttattcg acaacagcaa ccagatagca
    22861 aaatcacgca gcaaccagac ggtaaaactc gccgccgcca ctgccggtaa gacgaatttt
    22921 agcgatttaa ccacttcccg ggtgatatcg cgcttttccg ttagccgcga caacgtgggc
    22981 agcaaatata cgctgaacga tgccgtaatg aattgcaggt aggcatcgga aatactgctc
    23041 accccttgcc agatccccac ttcatcccag ctatactgcg ccgccagcag tttacgcatc
    23101 atgatgtaag caacaggcaa ggtcaccgac gtaatcaacg ccatgagcgt aaatttgctc
    23161 aactgccctg ccagaccgtt atcccagctg ggtttcagat agcttaacgg gatgacacca
    23221 cgtttgatca acatgatggc ggcaggaatt accaccagcg cgggaatcag cgccagaccc
    23281 agcaacgccc cttcataacc gcccaaacgg taactgacgt agtacgcgag aacgccaatc
    23341 aagctgccga caatcagcga taacgcatta cctgcggcat cgcgaaagcc tttcatcagc
    23401 gccagtaaca ggttgcccca ggcgatcccc atttgcacca gcgccactaa acgcaccagc
    23461 ccctgatagt cggtattacc aaacaatccc tggctgattg gcgcagctgc cagcacaaaa
    23521 accagcgcca tcagcgtaga gaaaccaagt accatcgctg atgaagtgcc gaccacgcgg
    23581 cgcagctgtt gcggattatc atggtactgg gcaacgtatt tggttacacc gttaaagatg
    23641 ccagccccgg caagcacgcc gagcacggta atcaactggc ggaaatttgc cgccagccca
    23701 agccccgccg gaccaaatga caccgccagc aacttaccga ccagtaaccc ggcaccaatc
    23761 ttgaccagtg tactggccgc cgtccacaag gacgcttttg ccaacgacat atcaggagaa
    23821 gtagttcaac aaagtcgcaa ttaccgtacg ctgattgacg ggcgacaggt tgtagaacag
    23881 cggcaggcgc agcaggcgct cgctctcttt ggtggtgtag cgatcttcac cgtggaactc
    23941 accaaagtgt tcccccgcag ggcaaccgtg cagcggaatg taatgaaaca ccgccatgat
    24001 ttccgcttct ttcagaaagt taatcaacgc gctccggtca tcaatatccc gcagtttaat
    24061 gtagaacata tgcgcgttct gcacgcagcc atcgggaatc gacggcagct cgatacgccc
    24121 ggctttcgcc agaggcgcta acgcatcgta gtaattttgc cacagcgcca gacgttgctg
    24181 gttgatacga tccgctgctt ccagttgcgc ccacaggtat gcagcttgca gatcggacat
    24241 caaatagctg gagccaatat cgcgccaggt atatttatcg acctgaccac ggaagaactg
    24301 gctgcggtta gtgccctttt cacggatgat ctcggctcgt tcgattaacg ctttatcgtt
    24361 aatcagcgtc gcgccgcctt caccacccgc cgtgtagttt ttggtttcat ggaagctaaa
    24421 gcagccaata tgaccaatgg ttcccagtgc acgccctttg taagtggaca tcacgccctg
    24481 agcggcatct tccaccacaa acaaattatg ctttttcgcc aacgccataa tggtgtccat
    24541 ttcgcaggcc acacccgcgt aatggaccgg cacgataacg cgcgttttgt cggtgatcgc
    24601 tgcttcaatc aacgtttcgt cgatgttcat ggtgtccggg cgaacatcca caaagacgat
    24661 tttcgcgcca cgcagcacaa aggcattggc ggtggagaca aaggtgtagc tcggcatgat
    24721 cacttcatcg ccaggctgga tatcgagcag cagcgccgcc atctccagcg aagcggtgca
    24781 ggacggcgtc agtaacactt tggcgctgcc aaaacgttgc tccagccact gctggcagcg
    24841 acgggtaaaa ccgccatcgc cacacagttt gccgctaccc attgccgact gcatatagtc
    24901 gagttcggtt cccaccaccg gcggtgcgtt aaatggaatc atgtgatcac ctgtataacc
    24961 agtacgcggt gctttctaca ttcgcaccac tttgtatgta tcgtttaagc gcggcggtgt
    25021 tgcccatttg ggtcgccacc cgcaaagttg ttttaccgcg agcatacgcc cagtttagcg
    25081 ccgtttgcat cagctcagca cctgcaccgc gtccagccag caggccaatt cgcgcatctg
    25141 tcgcattgag ttcccgtaaa gagacatagc cgcgaatatc gccggacgcc gcacgtagaa
    25201 tcagacattg atgatcaaag gtgccgcgca cggcattttc aatccactgt gcataaaagc
    25261 gaccgctggc gtcaggcgca taccacggcg cacgaaaacg gctttgcgca aatgcggcgc
    25321 tagctaactg acgtaatgcg ggaatatcgg tctcttgtgc cacgacagca ccgctatcac
    25381 tgacattgtt cacgggtagc gccaaatcaa cttcaccttc taccagggag aatcccagct
    25441 gttgcagggc atccagttca cccgtatttg atgccgcaat tttggcctgc acccgtgacc
    25501 acggcgctaa cgcgtctggc gtcaggagcg gtgcttcaga cgtaatgcgc acgatggcgc
    25561 tgttaacacc aaagaaggcg ttttcccagg ttagtggctc aatactggcg cggacgggca
    25621 cgaagtaact ccagcagata ttggccgtag ccagttttcg ctaatgaact ggcagcacgc
    25681 ttcacaccct cgtcatcgag ccagccgtta cgccaggcga tctcttccag gcaggcaatc
    25741 ttaaagccct ggcgtttttc tactgtctgc acaaaggtac tggcttcaat caggctgtcg
    25801 tgagtgccag tgtccagcca ggcaaatccg cgcccgagca gttcaacggt caggttgccc
    25861 gcctcgaggt acatctggtt gatggaggta atctccagtt caccacgctc cgacggcttc
    25921 acctgctttg cgtactccac gactttactg tcgtagaaat aaagcccggt caccgcccag
    25981 tttgacttcg gctgttttgg cttttcttcc agcgagatag cgcggaaatt gtcgtcaaac
    26041 tccaccacgc caaagcgttc ctggtccatc acctgatagc caaataccgt cgccccttcc
    26101 gtgcgcgccg caacatgacg cagcttcgga ctgaagccct gaccgaagaa gatgttatcg
    26161 cccagcacca gacaagaagg ttcaccgttg aggaaggttt caccgatgat aaaggcctgc
    26221 accagaccgt ccgggctggg ctgttcggcg tattccagct ggataccgaa ctcactacca
    26281 tcgcccagca ggcgctggaa ataaccttta tcttccggcg tagtgatgat gagaatttcg
    26341 cggataccgg ccaacatcag caccgacagc ggatagtaaa tcattggctt atcgtaaatc
    26401 ggcaacagtt gcttcgatac gccgcgcgta atcggatgca atcgggtgcc ggaaccgccc
    26461 gccaggataa tacctttcat gtttgcctcc gctgaaaatt agcctttcag acctaaacgc
    26521 tcgccctgat agctgccgtc ctgcacctgc ttccaccagc tttcattagc cagataccac
    26581 tgcaccgttt tacgcattcc actttcaaag gtttcctgtg gcagccagcc aagttcacgg
    26641 gcaattttcg aagcatcaat ggcataacgc agatcatgcc ccggacggtc agcgacaaag
    26701 gtgatcaagt cacgatagtg caccacgccg tgcggcttgt tcggagccag ttcttccagc
    26761 agctcgcaaa tggtttccac aacatcgaga ttcttacgct cgttgtgacc accaatatta
    26821 taggtttcac cgactttccc ggtggtcgcc acgcaataca gcgcgcgggc gtgatcttcc
    26881 acatacagcc agtcacggat ttgctgcccg ttgccatata ccggcagcgg tttacccgcc
    26941 agcgcgttga ggatcatcag cgggatcagt ttttccggaa agtggtaagg accgtagtta
    27001 ttcgagcagt tggtgataag cgtcggcaga ccgtaggtcc gcagccaggc gcgcaccaga
    27061 tggtcgctgc tggcttttga cgcggaataa gggctgctcg gcgcatacgg cgtggtttcg
    27121 gtgaagaaat catccgtcga gtgcaggtca ccatatactt cgtcggtgga gatatgatga
    27181 aaacggaacg ctgatttttt atcttccgtc agcgtattcc agtaagcccg cgccgcttca
    27241 agcaatgtat aagtcccgac aatgttggtt tcaataaacg ctgccgggcc gtcaatagaa
    27301 cggtcaacat ggctttcggc tgccagatgc atgacacagt ctggctgatg ctcagtgaat
    27361 acgcgtgcca gttctgcccg atcgcagata tcaactttct caaaggcaaa gcgatcgctt
    27421 tgcgcgaccg gtgccagcga catcaggttt ccggcgtagg tcagcttatc gaccactacc
    27481 accgcgtcgc tcgtttcgtt gatgatataa cgcaccagcg ccgagccaat aaacccggca
    27541 ccacctgtta tcagaatttt tctcatcagc gccagactcc tttggcatcg acgacatact
    27601 gctgatggac attgtcgcca ttgataactt taaactgact atgatcgacc agcatcacca
    27661 gcacgtctgc cgttgccagc gcctcgtcaa gctgcgccag agtacaaagc ccggtcagtt
    27721 ttttcggcaa ctggtggatg ttaggctcaa caaccagagt ttcgccgcta tgccactggg
    27781 cgatcagttc agcgatttcc atcgccgggc tttcgcgcag gtcatcaata ttcggtttaa
    27841 acgccagacc aaagcaggcg attttcagtt cactggcgcg tttatcggta gccgccaggc
    27901 aatcagccac cgccgctttc acctgatcga taacccagaa cggtttgtga tcgttcactt
    27961 cgcgcgcggt acggataagc cgcgcctgct gggggttctg tgccacgata aaccacggat
    28021 caacagcaat gcagtgaccg cccacgccag ggccaggctg aagaatatta acgcgagggt
    28081 gacgattcgc caggcgaatc agttcccaga cgttaatccc ctgatcggca caaatcagcg
    28141 acaattcatt agcaaaagcg atattcacat cgcggaagct gttttcggtg agcttacaca
    28201 tttccgccgt ccgcgagtta gtgacgacac actcaccttc gaggaaaatt ttgtacagtt
    28261 cgctggcgcg ggtcgaacaa accggcgtca taccaccaat cacgcgatcg tttttaatca
    28321 gctcgaccat aacctggccc ggtaacacgc gttccgggca gtaagcaatg ttgacgtccg
    28381 cctgctcgcc cacctgctgt gggaaagtga gatccggacg catctctgct aaccattctg
    28441 ccatcttctc ggttgacccc accggcgagg tggattcaag gatcaccagc gcgccttttt
    28501 tcagcactgg cgcaatggag cgagcagccg attcaacgta ggtcatatct ggctcatgat
    28561 cgcctttaaa cggcgtgggt acagcaatca gccaggcatc cgcttcaact ggcgtcgtgc
    28621 tcgctcgtaa aaaaccgcct tctacggcag tttttactac gctcgccaaa tcaggctcga
    28681 cgatatggat ttcgccacga ttgatggtat caaccgcgtg ttggttgata tcgacaccaa
    28741 tcacctgttt ttgccgtgag gcaaacgctg ctgctgtagg cagcccgatg taacccagtc
    28801 cgataacaga aatggtcgca aaactcatag tgatatccga ttatttttta acgcttccag
    28861 aatgcgagag catgcctgac catcaccata cgggttatgg gcgcggctca tagtttgata
    28921 ttcgttttcg tcttttaaaa gacgcgtcac ttcctcgaca attcgctgct tatccgtgcc
    28981 taccagacgc accgtacccg ccgtcaccgc ttccggacgc tcagtggtat cgcgcatcac
    29041 cagcaccggt ttccccagcg aaggcgcttc ttcctgaatg ccgcctgagt cggtcaaaat
    29101 cagccaggcg tggttcatca accagacaaa cggtaaatac tcctggggat cgatcagaat
    29161 gacatttttc acatgcccca gaatgcgatt gaccggctct ctgacgttcg ggttgagatg
    29221 caccggatag acaatctgga tgtcctggtg cgtggtggca atgtctgcca acgcgtggca
    29281 gatttcttca aaaccacgac cgaaactctc acgcctgtga ccggtcacca gaatcatctt
    29341 tttattgggg tcgataaacg ggtaatttgc caccagttct gaacgcagcg tgtcgctgct
    29401 catcacctga tcacgcaccc ataacagtgc atcaatgact gtattaccag taatgaagat
    29461 tcggctatcc gcaacgtttt cacgcagcaa gttttgccgg gaagtttcgg ttggagagaa
    29521 gtgatacatc gccagatgcc cggtcaatgt acggttagcc tcttccggcc acggcgaata
    29581 gagatcgccc gtgcgtaaac cagcctcaac gtgaccaaca ggaatacgct gataaaacgc
    29641 cgccaggctg gttgccagcg tcgttgttgt gtcgccgtga accagcacga cgtctggttt
    29701 gaactcggca agaataggtt ttagcccttc cagaatccga caggttatct ctgtcaggcc
    29761 ctgtcctggc tgcattatgt tgagatcgta gtcaggtaca atggaaaaga gtttcagcac
    29821 ctgatcgagc atctcccgat gctgcgcagt gacgcaaact ttagcctcaa aaaaaggagc
    29881 ttttgccaac gcatgcacca acggagccat cttgatggct tccgggcgcg taccaaatac
    29941 agtcagtact ttcacatcga ttctcttcga ataagcggcg agcgcctttg cgctcgccgc
    30001 agcagtgttg ctatttcgag caacggcggg ttaatgcgac accagccccg atcagccccc
    30061 cgacaatgcc ccacataatc atcaggaagg cacgacgtgg gctatcgcgt tttaccggtt
    30121 cttccggcgt acgcaaatag cgataggtct gaaaacgcgg atccagggtt ggaccaacat
    30181 tcagggtgtt taacatggcc cgattctgat catagtcgag atcaaaggcc ggaccgacgg
    30241 cctgtaaatt ttccagtcga gcctggagca ttggacgccc aagcaggaac atttctgaat
    30301 caggtaattc ctcggcaggc acatctgtcg cactgcgcga aatattatgc tgctcagcaa
    30361 ttttcagcgc ctgctcaatg ctgttcatcc ggcggtcgta gatggctttc gccacctctt
    30421 cctgacgctt cacctgagct ttcatctgga tggtgcgtgc cgcccatgcg cctttcagct
    30481 catcattcag atggctggct gcacgctggc tggcaaaagc aacatactga cgtaacaggt
    30541 tattagcgtc aggcgcggtt tcggcaataa gcttcacgct gtcattgacc gcgcgggtaa
    30601 agtctccggg gataaactgg atgttgttaa tcatttcatc cagcaacgcc gcatcggctt
    30661 tgctgttgcc caccatccgc tgtttgtaat agtcggtttg cagccagaac tctctgcggg
    30721 tatcccacga ggccagttgc ataacaaact ctttataggc ttcgtccatg accgatggtt
    30781 gatcggcaga agccatgttt gaacggacat ccaggttacg caaaaattgc tgctgcgagt
    30841 aatatccccc cagcatattc accgttggac gatcggtaat cgccgtcgag ctccactcct
    30901 gacgagcaaa aaaagtatac gccagcgcga ttaacgcaaa cgccagcccc atgccaataa
    30961 tccatagctt cccagcccac aaggtacgaa acaacccacg aatatccagt tcattttcag
    31021 cgtcttcggc cggtttccca ggcattggtt gtgtcatcac atcctcattt atttggttaa
    31081 attggggctg ccaccacgat ttctacgcag tctgcgtttt acgcgcttaa taaagcgagc
    31141 aactttccag gcacgcttaa tgcaatatcc atagaggaag aatgctagca aaaagagcac
    31201 cagcatgacc cactccggga caaaatgaga atattctgcc agcacgccaa tggaagcgag
    31261 cagtgctgcg gcaagggtaa tcagcacaaa cgcctgacgg gaagtaaacc cggcacgcat
    31321 gatcaaatgg tgaatatgct gacggtcagg agagaatggg ctcatgcctt tacgcaggcg
    31381 acggtacata atcgccacca tatccattag cggaatggcg attatccaca aagcggtaac
    31441 cgggctgatg ggatgggttt tgccctgggt cgtttcgagc aggatccaga taacggtaaa
    31501 accaatcagc gtactgcccg catcacccat aaagactttg tagcggcgac ccaggatacc
    31561 aaggttaagc atgatgtatg gcaggatggc ggcgatcatc gcaaagcacc agattgcgag
    31621 gctggtttgc ccgtcgaacc acaaaatcat accgattgct gcaaacgaga cgcaggacaa
    31681 cccgcccagc aagccatcaa tgccatcaac catgttgaac gcattaatgg ccgcccagac
    31741 ggcaaatagc gtcaggaagt aaccaaacgg tccgagcacc atctcccagg agccaaagat
    31801 ataacccagg ctactgagat aaagtttgcc gaacaccatc ataacaatgc caacagcggc
    31861 ctgtatggtg gcacggattt ttacgctgat atcaaaacgg tcatccagcg cgccaatgaa
    31921 aacaagcaca ccggcacaag cgagatagag agatgcatgc ggaatatagt aatcgacaat
    31981 tccgaacgtg aagcaaatcc ctgcgtaaac cgaaatcccc ccaacgagag gtatcaatcc
    32041 ctggtgacgt ttgcggaagt ttggtttatc cactaaaccg actttttttg ccaccttacg
    32101 ggcaaaaaac agaaacagtg tcgtgaataa aaaaatactg atgagatcag tactcactgt
    32161 cagtaaattc acaatgcatg ctctcagaga aaattattag cagaagtata accacgaaga
    32221 cctttattca gaagggaaag tccgttccga acccaccagt cctgtgcaaa aattaaccat
    32281 ttgatatgca aattgcataa catgccagca aattccagga attttcctaa catcgccagc
    32341 gcggcattaa gattacagat ataaaagcaa aacgccacgt aaacacgtgg cgtttttggc
    32401 ataagacaaa tttatgagcg tttcatcatt tcgaagaaat cgtcattggt cttggtcatg
    32461 gccagtttat taatgaggaa ttccattgca tcgatttcgc ccatcgggtg aatgattttg
    32521 cgcaggatcc acattttctg cagttcttcc tgagtcgtga gcagctcttc tttacgggta
    32581 ccggaacggt tgtagtcgat agccgggaag acgcgttttt cagcgatctt acgagagagg
    32641 tgcagttcca tgttgcctgt acctttaaac tcttcgtaaa taacttcgtc cattttagaa
    32701 ccggtatcga taagcgccgt cgcgataatg gtcaggctgc cgccctcttc cacgttacgc
    32761 gccgcaccga agaagcgttt cggacgatga agggcattgg cgtccacacc accggtcaac
    32821 actttacctg acgccggaac aacagtgttg taagcgcgcg ccagacgagt gatggagtcg
    32881 agcagaatga taacgtcttt cttgtgctca accaggcgtt tggccttctc gatcaccatt
    32941 tccgcaacct gaacgtggcg agatgcgggt tcgtcaaagg tagaagcaac aacttcacct
    33001 tttaccagac gctgcatctc ggttacttct tccggacgtt cgtcgatcag cagaaccatc
    33061 agcacacaat ccgggtggtt gtaagcaatg ctctgagcaa tgttctgcag cagcatagtt
    33121 ttaccggctt tcggcggtgc cacaatcaga ccacgctgac cacgaccgat aggtgatgcc
    33181 agatccagta cgcgagcggt taaatcttcc gtagaaccgt taccacgttc catacgcaga
    33241 cgagagtttg cgtgcagcgg ggttaagttc tcaaagagga ttttgttgcg ggcgttttca
    33301 ggtttgtcga agttaacttc gttaactttc agcagcgcaa aatagcgttc accttctttc
    33361 ggcgggcgaa tcttaccaga gatggtatca ccagtgcgga ggttgaaacg gcggatttgg
    33421 ctaggggaaa cgtagatgtc atcaggaccg gcgaggtagg agctgtctgc ggaacggagg
    33481 aaaccaaatc catcctgcaa tatctccagt acgccatcac caaagatatc ttcgccactc
    33541 tttgcgtgct gcttcaggat ggcaaaaata atgtcctgct tacgcatacg agccaggttt
    33601 tccagcccca tattttcgcc gagagtgatc agctcagaaa ccggcgtatt ctttaattcg
    33661 gtaagattca tagtggtgtg agttcttaaa cttggggtaa atctcgaact taatgttgtg
    33721 aatggtatgg caggatcatc catgcctgtt tacggtcatc gactcatgtc ttttcgctgc
    33781 ctggtcacag gagagtacgc agaactgaaa cgacaagacg gattgagtga cgagccagaa
    33841 atctgttcac ttcgcattta agataaaacg ggaagcgttg gaaattacaa gattcaaact
    33901 taataaggta tgtttaatac gaagtcaaca ctaagttagc atgactcacg ccgggcgtcc
    33961 agtttttagc gacggggcac ccgaacatga aattccctta cgccaggtta gcgtcgagga
    34021 actctttcaa ctgaccttta gacagtgcac ccactttggt tgccgccact tcaccgtttt
    34081 tgaacagcag cagagtcggg ataccacgga tgccatattt cggcgcagtg ccagggtttt
    34141 gatcgatgtt cagttttgca acggtcagtt tgccctgata ttcgtcagcg atttcatcca
    34201 gaatcggggc gatcattttg cacggaccgc accactctgc ccagaaatcg acgaggatcg
    34261 ccccgtccgc tttgagtaca tccgtgtcaa aactgtcgtc agtcaggtga ataattttat
    34321 cgctcatata taactccaca ggaataagcc tggcgtgttg gtttcgttgt tggtgtaaca
    34381 ttaaccaact aaaggttgac tttatttcac cggatacgct ttcgtaaagc aatagtaagc
    34441 tgatattcta ccacactatg agcaaaacac atttaacaga acagaagttt tccgacttcg
    34501 ccctgcatcc gaaggttgta gaaatccttg aaaaaaaagg gtttcataac tgtacgccca
    34561 ttcaggcact ggcccttccg ctgacgctgg cgggtcgtga cgtagccggg caggcgcaaa
    34621 ccggtaccgg gaaaacgatg gcgtttctta cgtcaacgtt tcattatctt ctctctcatc
    34681 ctgcgattgc cgatcgcaag gtgaatcaac cacgtgcctt aattatggca ccgacgcgtg
    34741 aacttgccgt gcagatccat gccgacgcag aaccgctggc ggaagctacc ggcctgaagc
    34801 tgggtctggc ttacggtggt gatggctacg acaaacagct gaaagtgctg gaaagcggcg
    34861 ttgacattct gattggcact acggggcgtt taattgacta cgccaagcag aaccacatta
    34921 acctcggtgc cattcaggtg gtggtactgg acgaagccga tcgcatgtac gatctgggct
    34981 ttattaaaga tatccgctgg ctgttccgcc gtatgccgcc tgcaaaccag cgcctcaaca
    35041 tgctgttctc cgccacgctt tcgtaccggg tacgtgaact ggcgttcgag cagatgaaca
    35101 atgccgaata tattgaagtg gaaccggaac agaaaacggg ccaccgtatt aaagaagagc
    35161 ttttctaccc ttctaacgaa gaaaaaatgc gtttgctgca aacgctgatc gaagaagagt
    35221 ggccagacag agcgattatt ttcgccaata ccaaacaccg ttgtgaagag atctggggcc
    35281 acctggcggc agatggtcat cgtgtcggtt tgttgacagg cgatgtcgcg cagaaaaaac
    35341 gtctgcgtat tcttgatgaa tttacccgtg gcgatctgga tattctggtt gccaccgacg
    35401 ttgccgcgcg tggtttgcat attccggcag tgacgcacgt ctttaactac gatttacccg
    35461 atgactgtga agattacgtt caccgtattg gtcgtacagg tcgcgcaggc gcaagcggtc
    35521 actctatcag cctggcgtgt gaagagtatg cattgaattt gcctgctatt gagacctata
    35581 ttggtcactc aattccggta agcaaataca atccggacgc attgatgacc gatctgccaa
    35641 aaccgctgcg cctcacgcgc ccacgtacag gcaatggtcc gcgtcgtact ggcgctccgc
    35701 gtaatcgtcg tcgttcaggt taaataaaat gtcatactca aaatcattca aactgtatca
    35761 agatggcaag agaatgaatc ccgatgagca tactgtagta agtgattcag gtgaaagaat
    35821 gcagccaaca cagagacaga ttgaaggatg aagagtatgg gttccacctc gtcgctgtat
    35881 gcagccattg atctcggttc gaatagtttt catatgctgg ttgtgcgcga ggtggctgga
    35941 agcatccaga cgctgacgcg aataaaacgc aaagtgcgtc tggctgctgg cctgaatagc
    36001 gaaaatgccc tgtctaatga agcaatggag cgcggttggc aatgtctgcg cctgtttgct
    36061 gaacgtctgc aagatatccc tccctcgcaa attcgcgttg tcgctacggc gacattacgt
    36121 cttgccgtca atgcgggtga ttttattgcc aaagcgcagg aaatcctcgg ttgtccggta
    36181 caggtgatca gcggtgaaga ggaagcacgt ctgatttatc agggcgttgc tcacacaact
    36241 ggcggtgccg atcagcgcct ggtggtggat ataggcggtg ccagtactga actggtaacc
    36301 ggcacgggtg cacaaaccac ctcgttgttc agcctgtcga tgggctgcgt cacctggctg
    36361 gaacgctatt ttgccgatcg taatctgggg caggaaaatt ttgatgctgc agaaaaagcg
    36421 gcacgcgaag tgttacgtcc ggttgccgat gaattacggt atcacggctg gaaagtgtgc
    36481 gttggtgctt ccggcaccgt gcaggcgtta caggaaatca tgatggcaca ggggatggat
    36541 gaacgcatta ccctggaaaa gttgcagcaa ttgaaacagc gagccattca ttgcggtcgg
    36601 ctggaggaac tggagattga cgggctgacg ctggaacgtg cgttagtgtt cccgagtggt
    36661 ctggcgatcc tgatcgccat ttttaccgaa ctgaatattc agtgtatgac cctggcgggc
    36721 ggtgcgctgc gtgaaggcct ggtctacggc atgttgcatc tggccgtcga gcaggatatt
    36781 cgcagccgta cgctgcgtaa tattcagcgc cgctttatga tcgatattga tcaggcgcag
    36841 cgcgtagcca aagttgcggc taacttcttc gatcaggtgg aaaaggaatg gcatcttgaa
    36901 gcaataagcc gcgatttgct catcagcgcc tgtcagcttc atgaaatcgg cctgagcgtt
    36961 gactttaaac aagcgccgca acacgctgct tatctggtgc gtaatctgga tcttcccggt
    37021 tttacccccg cacagaaaaa attgctggcg acgctactgc tcaaccagac taatccggtc
    37081 gatctctcat cgctgcatca gcaaaatgcc gtaccgccgc gcgtcgcaga acaactctgc
    37141 cgtttactcc gcctggccat catttttgcc agccgtcgcc gtgacgatct cgtgccagag
    37201 atgacattac aggctaacca tgaactgttg accttgacgc ttccgcaagg ttggctaacc
    37261 caacatccgc tgggtaaaga gattattgct caggaaagcc agtggcagag ctatgtccac
    37321 tggccgctgg aagtgcatta atgagtaagt gccggatgcg atgctgacgc atcttttccg
    37381 gccttgatta tttccctcgt tttgccgcca tcatcgcttt cagattcgcc agatggcttt
    37441 gccctttctg catccgttct tcggcgctga ccactttgcg ctcctgttcc caaatcagat
    37501 catcctgcgg cagctccagc aaaaagcggc tcggctccgg gcgcaccagc tcgccgtact
    37561 gacggcgttc tttacacagc gtaaaggtca attccttctg ggcgcgggta atgccgacat
    37621 aggccagccg ccgctcctca tcgatattat cttcatcgat gctgctctgg tgcggcaaaa
    37681 acccttcttc cataccgacc atgtagacat aaggaaactc cagccctttt gacgcgtgca
    37741 gagtcatcag ttgcacctga tccagctctt cttcactctc accacgctcc atcatgtcgc
    37801 gcaaagtaaa gcgcgtcacc acctgggtga gcgtcatcgg ctcatccagt tcgctgcctt
    37861 ccagcatctc cgtcatccag ctaaacagtt ggttgacgtt cttcatgcgc atttcggcgg
    37921 ctttcgggct gggcgatgtt tcgtacagcc aggattcata atccatgccg tggatcagat
    37981 cgcgcaccgc ggcaatcggc tcccgctccg ccagacgctg gatttctgcc aaccagtgag
    38041 taaagcgggt caatgcttca taaccacgtc cgctaagcgt ctgactcagg cccatatcaa
    38101 agctggcggt aaacatgctt ttattgcgcg tcatcgccca ctcacccagc tttttcagcg
    38161 tcgccgggcc aatctctctc ttcggcgtgt taacgatacg cagaaatgcg ctgtcatcgt
    38221 ccgggttagt cagcacgcgc agataagcca gcaagtcctt gatttcagga cgagagaaaa
    38281 acgacgtacc accagaaatt ttgtacggga tgcggttttg catcaggaat ttttcaaaca
    38341 ctcgcgactg atggttaccg cgataaagaa tagcgtaata tttgtactgc gttttattga
    38401 cgaagtgatg cgcaatcagt tcaccagtga cgcgctcggc ttcatgttct tcgttattcg
    38461 cgcttaaaac tttaagctct gtgccgtaac ccagttcgga gaacagacgc ttctcaaaga
    38521 cgtgcgggtt attggcgatc aggatgttcg ccgctttcaa aatacgtccg gaagagcggt
    38581 agttctgctc aagcttaatc accttcagcg ccggaaaatc ctgactcagc agcaccaggt
    38641 tttgtggacg tgcgccgcgc caggagtaga tcgactggtc gtcatcgccc accacggtaa
    38701 agcgcgcgcg gctgcccacc agcagtttca ccagttcata ctggctggtg ttggtgtcct
    38761 gatactcatc caccagcaga tagcgaattt tgttctgcca gcgctcacgg acttcttcat
    38821 tgcgttgcag cagcaacgtc ggcaataaaa tcagatcatc gaagtcgaga acattgcacg
    38881 ctttcaggtg tgcatcatac agcccataac aatgggcaaa aatacggtcc cgctcgccaa
    38941 tcgcactggc tgccgcctgg gacggtgttt tgagatcgtt cttccagtta gagatggtcg
    39001 aaatcaactg ttgcaggaga actttgtcat cttcaatcag cccttcggtc aactctttga
    39061 gcaacgccag ctgatcggta tcgtcaaaca gcgaaaagtt cgccttcatc cccagcgccg
    39121 catactcacg cttgatgata tccagcccca gcgtgtggaa ggttgagatc atcagcccac
    39181 gcgcctcttt gcgacccagc gtctgtccga cacgctcttt catctcgcgc gccgccttat
    39241 tagtaaaggt caccgccgcg atatgtcgcg cctgataacc gcaaccgcgg atcagatggg
    39301 cgattttatt ggtgatcaca cgagttttac cggaacccgc gcccgccagc accaggcagg
    39361 ggccggtaac gaattcgaca gcttgttgtt ggccggggtt tagacgcata ggtgtattgc
    39421 tcaatcttcg aacgggggga ggattgtagc agaatcgcat ttgaatgtga ccgcgtgtaa
    39481 gccgctaacc ggttggctgc ggatgtttaa atcagagagg gaagaccgaa accggagttc
    39541 atcgtgaatc cattagactt agaaaatatc gggtcgtaac gttgtgttat tactttttca
    39601 gagtttacta aggagaggtg tactcgtggc gattaatttc agtcctaagg ttggtgaaat
    39661 actggaatgc aactttggta actatcctgt tagccagaat ggacagttca gcaccacgta
    39721 ttacgatggc cgcattcctc ctgaaatgat aaaaaacagg ttagt
//
LOCUS       DAJTSL010000017        37610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTSL010000017 DAJTSL010000000
VERSION     DAJTSL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.017"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1985
                     /locus_tag="OO433_000783"
     CDS             <1..1985
                     /locus_tag="OO433_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCR6049358.1"
                     /translation="VGAFLAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDT
                     FNTEQVEVIKGPSGTDYGHSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVN
                     QVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHN
                     TPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDI
                     NDNTTIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILT
                     NQTNLTSTFYTGSIGHDVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTR
                     NGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCP
                     AGVAKGSPVTTVDTAKSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQS
                     GSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQY
                     GKKRVEGYEISVAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLW
                     SQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLN
                     VYNLFDTDYVASINKSGYRYHPGKPRTFLLTANMHF"
     gene            2027..2704
                     /gene="ybiX"
                     /locus_tag="OO433_000784"
     CDS             2027..2704
                     /gene="ybiX"
                     /locus_tag="OO433_000784"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6049359.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            2778..3044
                     /gene="ybiI"
                     /locus_tag="OO433_000785"
     CDS             2778..3044
                     /gene="ybiI"
                     /locus_tag="OO433_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6049360.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3309..3569
                     /locus_tag="OO433_000786"
     CDS             3309..3569
                     /locus_tag="OO433_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6049361.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLTEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3797..4882)
                     /gene="hcxB"
                     /locus_tag="OO433_000787"
     CDS             complement(3797..4882)
                     /gene="hcxB"
                     /locus_tag="OO433_000787"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6049362.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGYLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(5023..5985)
                     /gene="ybiB"
                     /locus_tag="OO433_000788"
     CDS             complement(5023..5985)
                     /gene="ybiB"
                     /locus_tag="OO433_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6049363.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLEATGEAATISDGLARVNQAF"
     gene            complement(6013..8163)
                     /gene="dinG"
                     /locus_tag="OO433_000789"
     CDS             complement(6013..8163)
                     /gene="dinG"
                     /locus_tag="OO433_000789"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6049364.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            8283..8765
                     /gene="ybiA"
                     /locus_tag="OO433_000790"
     CDS             8283..8765
                     /gene="ybiA"
                     /locus_tag="OO433_000790"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6049365.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(8997..10361)
                     /gene="rhlE"
                     /locus_tag="OO433_000791"
     CDS             complement(8997..10361)
                     /gene="rhlE"
                     /locus_tag="OO433_000791"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR6049366.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSSDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10590..11261
                     /gene="cecR"
                     /locus_tag="OO433_000792"
     CDS             10590..11261
                     /gene="cecR"
                     /locus_tag="OO433_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6049367.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNTTTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            11264..12259
                     /gene="hlyD"
                     /locus_tag="OO433_000793"
     CDS             11264..12259
                     /gene="hlyD"
                     /locus_tag="OO433_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6049368.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLKLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQVKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            12252..13988
                     /locus_tag="OO433_000794"
     CDS             12252..13988
                     /locus_tag="OO433_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6049369.1"
                     /translation="MNDAVITLNGLEKRFPGMGKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATMIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELASEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13981..15114
                     /locus_tag="OO433_000795"
     CDS             13981..15114
                     /locus_tag="OO433_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6049370.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            15125..16231
                     /locus_tag="OO433_000796"
     CDS             15125..16231
                     /locus_tag="OO433_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6049371.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIATNYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(16193..16603)
                     /locus_tag="OO433_000797"
     CDS             complement(16193..16603)
                     /locus_tag="OO433_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6049372.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16736..17487
                     /locus_tag="OO433_000798"
                     /pseudo
     CDS             16736..17487
                     /locus_tag="OO433_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            17484..18725
                     /gene="clsB"
                     /locus_tag="OO433_000799"
     CDS             17484..18725
                     /gene="clsB"
                     /locus_tag="OO433_000799"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6049373.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNKLTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWHPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18725..19681
                     /locus_tag="OO433_000800"
                     /pseudo
     CDS             18725..19681
                     /locus_tag="OO433_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(19717..20430)
                     /locus_tag="OO433_000801"
     CDS             complement(19717..20430)
                     /locus_tag="OO433_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6049374.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLVIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(20636..21340)
                     /locus_tag="OO433_000802"
     CDS             complement(20636..21340)
                     /locus_tag="OO433_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6049375.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(21477..21929)
                     /gene="moaE"
                     /locus_tag="OO433_000803"
     CDS             complement(21477..21929)
                     /gene="moaE"
                     /locus_tag="OO433_000803"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6049376.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFAGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(21931..22176)
                     /gene="moaD"
                     /locus_tag="OO433_000804"
     CDS             complement(21931..22176)
                     /gene="moaD"
                     /locus_tag="OO433_000804"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6049377.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(22169..22654)
                     /gene="moaC"
                     /locus_tag="OO433_000805"
     CDS             complement(22169..22654)
                     /gene="moaC"
                     /locus_tag="OO433_000805"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6049378.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(22657..23169)
                     /gene="moaB"
                     /locus_tag="OO433_000806"
     CDS             complement(22657..23169)
                     /gene="moaB"
                     /locus_tag="OO433_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6049379.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(23191..24180)
                     /gene="moaA"
                     /locus_tag="OO433_000807"
     CDS             complement(23191..24180)
                     /gene="moaA"
                     /locus_tag="OO433_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6049380.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(24168..24312)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            24577..25485
                     /gene="yvcK"
                     /locus_tag="OO433_000808"
     CDS             24577..25485
                     /gene="yvcK"
                     /locus_tag="OO433_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6049381.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(25677..27698)
                     /gene="uvrB"
                     /locus_tag="OO433_000809"
     CDS             complement(25677..27698)
                     /gene="uvrB"
                     /locus_tag="OO433_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6049382.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(28277..28954)
                     /gene="bioD"
                     /locus_tag="OO433_000810"
     CDS             complement(28277..28954)
                     /gene="bioD"
                     /locus_tag="OO433_000810"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6049383.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(28947..29702)
                     /gene="bioC"
                     /locus_tag="OO433_000811"
     CDS             complement(28947..29702)
                     /gene="bioC"
                     /locus_tag="OO433_000811"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6049384.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(29689..30843)
                     /gene="bioF"
                     /locus_tag="OO433_000812"
     CDS             complement(29689..30843)
                     /gene="bioF"
                     /locus_tag="OO433_000812"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6049385.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYNTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(30840..31880)
                     /gene="bioB"
                     /locus_tag="OO433_000813"
     CDS             complement(30840..31880)
                     /gene="bioB"
                     /locus_tag="OO433_000813"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6049386.1"
                     /translation="MAHRPRWTLSQVAELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVQGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            31967..33256
                     /gene="bioA"
                     /locus_tag="OO433_000814"
     CDS             31967..33256
                     /gene="bioA"
                     /locus_tag="OO433_000814"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6049387.1"
                     /translation="MTKDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LIDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEVRQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            33315..33791
                     /locus_tag="OO433_000815"
     CDS             33315..33791
                     /locus_tag="OO433_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6049388.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVEHIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            33943..35226
                     /locus_tag="OO433_000816"
     CDS             33943..35226
                     /locus_tag="OO433_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6049389.1"
                     /translation="MNTFSVSRLALALAFSVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35367..37604)
                     /locus_tag="OO433_000817"
     CDS             complement(35367..37604)
                     /locus_tag="OO433_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6049390.1"
                     /translation="MFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSSHNTSGNMD
                     KLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGTINGDDHVF
                     GLSAAQRYGGIFVLPHIAIIHQYMREMMAGGGKMILGSDSHTRYGALGTMAVGEGGGE
                     LVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVG
                     PGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYY
                     DGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAHGKAKLSLL
                     DKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPSSQPVFMDL
                     AKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREGSKPANGQM
                     SAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQ
                     PLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRSNPIGLAEF
                     TLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMV
                     YAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEV
                     GDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFN
                     KNRQM"
BASE COUNT         8811 a        10183 c         9740 g         8876 t
ORIGIN      
        1 gcgtgggtgc gtttttggcg ggtgagaacg gtaactccac cactggcgac gccatttata
       61 tgcgtggtgc cgatacctct aacagtattt atattgatgg cattcgcgat atcggcagcg
      121 tctcgcgcga caccttcaat accgagcagg tcgaagtgat taaagggccg tccggcaccg
      181 actacgggca cagcgcaccg acgggctcga tcaatatgat cagcaaacag ccgcgcaatg
      241 attccggcat tgacgcctcc gccagtattg gcagcgcctg gttccgccgc ggcacgctgg
      301 acgtcaatca ggtcattggt gataccaccg cggtgcgcct gaatgtaatg ggcgaaaaaa
      361 cgcacgatgc cggacgcgac aaagtcaaaa atgagcgtta cggcgtcgcc ccttctgtcg
      421 cttttggcct tggtacagcg aatcgtttgt atcttaatta tctgcatgtc acccagcaca
      481 acacgccaga cggcggcatt ccgaccatcg gtttgccggg ctattctgcc ccatctgcgg
      541 gaacggcggc cctgaatcat tccggaaaag ttgatactca taacttttac ggcacggatt
      601 ccgattacga cgattcgacc accgacaccg ccaccatgcg ttttgagcac gacatcaacg
      661 ataacaccac cattcgcaat actacccgtt ggtcgcgcgt aaagcaggat tacctgatga
      721 cggcgattat gggcggggcg tcgaatatta ctcagcccac cagcgatgtg aatagctgga
      781 cctggtcacg cacggcgaat accaaagatg tgagtaataa aattctcacc aaccagacca
      841 acctgacctc gacgttctat accggttcta tcggtcatga tgtcagtacc ggcgtggaat
      901 ttacccgtga aacgcagact aactacggcg ttaatccggt gacgttaccc gcggtaaata
      961 tttatcatcc tgacagcagc attcatcccg gcggcctgac gcgcaacggc gcaaacgcca
     1021 atggtcagac ggataccttc gcaatttacg cctttgatac gctgcaaatc acccgtgatt
     1081 ttgagctgaa cggcgggatc cgtctggata attatcatac tgaatatgac agtgccaccg
     1141 cctgcggcgg cagcggacgc ggtgccatca cctgcccagc tggtgtggca aaaggttctc
     1201 cggtcaccac cgtcgacacc gccaagtcgg gcaatctggt gaactggaaa gccggggcgc
     1261 tgtatcacct gacggaaaac ggtaatgtct atattaacta tgccgtttcc cagcagcctc
     1321 cgggcggcaa caacttcgcc cttgcgcagt ctggcagcgg taacagtgcc aaccgcaccg
     1381 attttaaacc gcaaaaagcc aacaccagcg agattggcac caaatggcag gttctggata
     1441 aacgcctgtt gctcaccgcc gcactgttcc gtactgatat cgaaaatgaa gttgagcaaa
     1501 atgatgacgg aacttactcg caatacggta agaaacgtgt cgaaggctat gagatatccg
     1561 tggccgggaa tatcactccc gcgtggcagg tgattggcgg ctatacccag caaaaagcaa
     1621 ccatcaaaaa cggcaaagat gttgcccagg atggttcctc atcgctgccg tataccccgg
     1681 agcacgcctt caccttatgg agccagtatc aggcaaccga tgatatctct gttggcgcgg
     1741 gcgcacgcta tatcggcagt atgcataaag gttcagacgg cgcggtggga acgccagcgt
     1801 ttaccgaagg ttactgggtc gccgatgcca aactggggta tcgggttaat cgcaatctcg
     1861 acttccagct aaacgtttac aacctgtttg ataccgatta cgtcgcctca atcaacaaga
     1921 gcggctaccg ttatcacccg ggcaagccaa gaaccttctt gctcacagcc aatatgcatt
     1981 tctgattcag atgtggggcg caggccccac tttttggaga aattgtatga tgtaccacat
     2041 tcccggcgtg ttatcgccac aggacgtcgc tcgttttcgc gaacaactgg aacaagccga
     2101 atgggtggat ggacgcgtca ccaccggcgc acaaggtgcg caagttaaga acaatcaaca
     2161 ggtagacacc cgcagcacgt tatacgccgc cctgcaaaat gaggtgctga acgcggttaa
     2221 ccaacatgct ttattctttg ccgcggcctt gccgcgtacc ctttccacgc cgctgtttaa
     2281 tcgctatcag aacaatgaaa cctatggttt tcatgtggat ggcgcagtac gcagccatcc
     2341 acaaaacggc tggatgcgca ctgacctttc tgccacgctg tttttaagcg atccacaaag
     2401 ctacgacggc ggcgaactgg tcgttaatga caccttcgga caacatcggg taaaactccc
     2461 ggcaggtgat ctcgtgttgt atccctccag cagcctgcat tgcgtgacac ccgtaacccg
     2521 cggcgtacga gtggcatcat ttatgtggat ccagtcgatg atccgcgatg ataaaaagcg
     2581 cgccatgctg tttgaactag acaaaaatat tcagtcgctg aaaagccgct atggtgaaaa
     2641 tgaagagatc ctgtcgctgc tcaatcttta tcataatctg ctgcgggaat ggtcggagat
     2701 ctgacgcttg aactgcccag gttcgtccct atattagcta cacttaactg tacaagcatt
     2761 gatatgggga ggacgatatg gcatccggtt gggctaacga tgacgccgtc aacgaacaga
     2821 tcaacagtac aattgaagat gcgattgccc gcgctcgggg tgaaattccg cgtggcgaaa
     2881 gcctgtatga atgtgaagag tgcggtgccc ccatcccgca agcccgtcgg gaagccattc
     2941 ctggcgtgcg cttatgtatt cattgtcagc aggagaaaga tttacaaaaa ccagcgtata
     3001 caggatataa tcgcagaggt tcgaaagaca gccagttacg ttaactacaa ctggcgagat
     3061 gcatagcgag tataatttgt attttgcgtg gcattccgtg cctttaacgc tgctgtgact
     3121 gccacgcggc ataaaacaag tccataagta caacaaataa atggtttatc agtatgttag
     3181 atgtcaatca atttatttga acaaggcggt caattctctt cgattttatc tctcgaaaaa
     3241 aaacgtgata ctcatcacat cgacgaaaca acgtcactta tacaaaaatc acctgcgaga
     3301 gattaattat gaaaactatc aatactgttg ttgctgctat ggctctttca actctatcat
     3361 ttggcgtgtt cgcggcggaa ccggtaacgg catcccaggc acagaacatg aataaaatcg
     3421 gcgtggtttc tgccgatggc gcatccaccc tcgatgccct ggaagcgaaa ctgactgaga
     3481 aagccgcggc agccggtgcc agtggataca gcatcacttc tgccaccaac aacaataaat
     3541 taagcggtac tgcggtaatt tataagtaat tcgtaggacg gataaggcgt ttacgccgca
     3601 tccggcaaca gatgcctgat gcgacgctac cgcgtcttat caggcctaca aaatccgaac
     3661 cgttggacgg atcaggcggt tacgctacgt ccagcacaag tatttccctg tctgtttgcc
     3721 gacagacgca tatgctctaa ccctcattga tcctatatta cccttgtttg cccgtccgtt
     3781 actagacggg ctttttttag ctggctaact gctgacagaa agcctgcaac gtctcttccg
     3841 acataccaat ctgccgcgcc gcatcacaaa tggcctgcca gcttcccgca tccaatggaa
     3901 tcccctgctc ctgccgttcg cgacgcgtgt tcacttccca ctcgcccggt agcaaaatcg
     3961 gcttatcgtc atcatgcggc gaggctttca cccactcggc aaaggcttca gtctgcgcgc
     4021 tacaatccgg cgcgccgaac agttccgggt tgatgatgat agtggtcatg cagttaagaa
     4081 tggcatcggg actggtttgt aacgtttcct gatgcgtcgt tttaccgcca gaaagcgccc
     4141 cgccaagaat ttcacacatc gctgcaaggg cgtagccttt atgttcggca aaggtcaaca
     4201 gcgaccccaa cggcgactcc tgcattaccg ccggattggt cgtcggcacg ccgttaacgt
     4261 caatcaggca acctggcggc acggggacgc ctttatgcca ggcgacgcga gttttaccaa
     4321 atgcgatggc gctggtggcg taatcaagca acagcgggaa attatcttta cgagggaaaa
     4381 ccacacagaa cggattggtg ccaaagcggc tgtcgcgacc gtggaacggt gcgaccattg
     4441 gaataccgac cacgctaaca aagtggatag agacaaaccc cgccgctgca cactgctccg
     4501 cccagtaacc gatacggccg atatgatgcg agttatgtag cgccacggca gcaataccgt
     4561 gctgatgcgc tttctcaatc cccagcgcca tcgcttcgtg tgccgcgacc tgaccgaatg
     4621 cgcgatcgcc atcgagcgtg actgccgccc ccgcctcttt aacgattttg gcatgatggt
     4681 taatttgcag atacccctga ctccaggaac gcacatagct tgggatcatg ccaataccat
     4741 gagaatcatg ccctgccagg tttgccgcga ttaaatgatc ggccactaat ttcgcttctt
     4801 gttcctcgct acccatctga cgaaatacag cctgaataaa actgtgcagc gtctgagcat
     4861 caaagcgatg accactttcc atggttaact cctgtttatg ttatgtgttt gttgtttttt
     4921 tatgctgcac gccgggcgcg ggaaggtcaa taaagatgca ggaatttcat attgcggtgg
     4981 ggtaaaagcc ggatgacatg gctcatccgg ctgagaaaag aattaaaatg cctgattaac
     5041 gcgcgccagg ccgtcgctga tagttgccgc ttcacccgta gcctccaggc agcaagccat
     5101 ctggattttc agcgattcgg gaatcggttc gctgccagca aggcaacgct caatccactg
     5161 cgccgtggtt tctgggtctt ttgcttgtgg cagtaactcg ctaccagcag tgtcctgttt
     5221 ttcatacagc acccgcatcc cttcacggtc aatgagattg atctgcgggc agcgctgcgg
     5281 attagcatac acttcgcctt cagtaccatg cattagtagc gcccgcccgc cgatatcgct
     5341 aaagaacttc gcgactcgcc cgatgtattc cggatgcgaa acgctggaaa gacgcagcgc
     5401 ctcaccttcg gcaaatggcg tcgccagttt cgccagggta tgtgcactgt tacgcacgcc
     5461 catccgccag cgcatcgcca gttgtttttc cagcggcggg caaaacgcgc cgacaggcat
     5521 aaacaccggt tgatgttcgt cgagcttcgc ctgcgcctgc ccgccgtgca acgttggtgt
     5581 aatacccatc agttcgaaaa tggtttctgt cagcacgcga gttggatctt cgctaacccc
     5641 gtgaaccacc acaggaaaac cgagtttgtg caggagaata gccaacaacg gcgtcaggtt
     5701 ggcctgttta cgcgcgccgt tgtaactggg gatgacaatc ggcatcggct tgcctgcagg
     5761 cggcgtcagc ttgatggtgt gattttgcat ggcttcgtaa aagccgagca tctctgcttc
     5821 cccttcccct ttgatacgta gcgcaatcaa tacgccgccc aactcaaggt cagggacttc
     5881 accattgagc atatgggcat acagaccgcg cgcagtatcc cggtctaaat cgcgcgcgtg
     5941 gtttttcccg cgcccgatct ctttaatgat tttgcgatag tccatttacg actccttacc
     6001 tgactcacat cattaacgcc gccgacggcg tggggatttc gttttttctt tctttttaac
     6061 tataccttca gggacttccg gttgctctat cggaaatacc ggtaatgcat ccagtagtcg
     6121 cttgccataa tttttggtca gcaagcgttt gtcgtagata accacttcgc cccagcaacc
     6181 gtggcttcga atcagtcgcc caacctgctg aatcaggtta aacgaggcgc tcggcaggct
     6241 ttgcacctca aacggatagc ggttgaggct tttcagccat tccccttcgg tgatcaccac
     6301 cgggctgtcg atgggcggaa aagcgatttt gtggatatgc acctggctga gcagatcacc
     6361 tttcaaatca agcccttcgg caaatgactg taagcccacc agcacactac gctcaccgtt
     6421 ggcaacgcgt ttgcggtgca gttcaactaa gcgataacgc ggctgatcgc cctgcaccag
     6481 cagcatcagg cgtaaatccg tcacatagtc gagaaagcgc tgcatcgccc gtccgctggc
     6541 aaacagtacc aacataccga gatgtttttt gctctccacc tgctcacgga aaaaggccgc
     6601 catttcggca atatgctgct cttcgttgtc gatggaaggc tcaaagcgca tccggggaat
     6661 aacaattttg ccctgttcgc agtggttaaa gggggaatcc agcgccacaa aacggtcgcc
     6721 cgctttctct ttcagcccgc tcatctcctg caaacgcgaa aaactgttca gcgaacgcaa
     6781 ggttgcggag gtgacaataa tgtgcggaat actgcgccac agcagccttt ccagctgatc
     6841 gctgacacgt attcccacgc agtgaaacca gagatgcagc tgcccttcgc gctcttcccg
     6901 cgtcgcccat ttggtcaccg gcgcaccgga agattgcgcc agagacgcca gccgccagag
     6961 tttgctttgc gcctcgaaca tccccaacgc gcggttcatc tgcaaaatca accgatgcag
     7021 acgcacaata tcgtggctgc cggttttctc acttaaatcg ttaagaaata actccgccag
     7081 accacgcagc atctcggtga gttttgccag ccgctggcag atctccagca cttcatcagg
     7141 cagttcgccc atcgcaaaac ggtgctctgc ctcctgcccg gcaggcatgt agagattgag
     7201 aatgttgttt aacgaggcga taagctcata caactcttca caatgcgcat tcaaacgttc
     7261 agggatcgcc agcggtggga tggtcttcgg gcgaaactgc tccatgcagg tagcgaccag
     7321 tttcgtgaac aagtccagct gtagtcgata ccagggcgcg gtgatttcag cgctcatctc
     7381 cagcgcatcc cgggccacat ctggcaggtg atgaccttcg tccagcacca gcagcagatt
     7441 tttcgggtca ggcagtacgg cttcgctttc catcgccgcc atcaccagcg catggtttgc
     7501 caccaccact tccgcttcct gaatctcccg acgagcaaca aaaaacgggc attcacgata
     7561 gtagtaacag ttacggttaa ggcagctggc tttgtcggta ctcaaacgac gccagagatc
     7621 atcatctata gcgatatccg tatgatcgcg cagaccatcc catttataag tgtcgagatc
     7681 gcccttcagc ttcgcacaac gtttttgctc ttcctgatta tttggcgtca gttcgtcgtc
     7741 aagaaacgcc agcagatcct gttgcgtggg ttcagtgctg gcgagcgccg tcagattacg
     7801 cggacaaacg tagcgcccac gcccgaaagc ggcagtgaat ttaagatcgg gaatgatctt
     7861 tttcagcagc ggtaaatctt tgctgtaaat ctgatcctgc aacgccacgt tggcggtact
     7921 caccaccagc gttttttgct cttcgcgggc aatggcgatg ccggggatca aataggagag
     7981 cgttttccca acgccggtgg gggcttcaat cgccagatgc cgcccttctt ctccggccag
     8041 cgttttggcg acgtccgcaa tcatctgccg ctgcggcgca cggggaataa agtcggggat
     8101 ctgttcctga agcgccttat accaggcggc aatttgcgct ttaagcgcgg cggttaatgc
     8161 catgagaaaa cctgaaatac tgtataaaca gccaatattg tggcattttt cggttttcag
     8221 gggtaatggt ttttctgttt ctggaaagcg gctcgcaata accaccaaca taatgcggtt
     8281 taatgcccgt tcgagcacaa aggatccaac acgtcatgca agacaccatc ataaatttct
     8341 acagcaccag cgatgactac ggtgattttt ctaactttgc cgcctggccc atcaaggttg
     8401 acgggaaaac ctggcccacc tccgaacact attttcaggc acaaaaattc cttgatgaaa
     8461 aataccgcga agagatccgt cgggtttctt cccccatggt cgccgctcgc atggggcgta
     8521 atcgttccaa gccacttcgt aaaaactggg agtcggttaa agaacaggtg atgcgtaaag
     8581 cacttcgtgc taaattcgaa cagcatgcag aactgcgtgc gctcttgctg gcaaccgcgc
     8641 ccgcaaaact ggttgagcat acggaaaacg atgcttactg gggagacggt ggtaatggta
     8701 agggcaagaa tcgactgggc taccttttaa tggagttgcg cgaacaattg gctatagaga
     8761 agtaacgtct cggtggctta tgggcggtaa aacagataaa ttttttcccg tgttctcaaa
     8821 ttgctgacaa gtgcttgttg tgaaatgccg gatgcggcgt aaacgcctta tccggcctac
     8881 aaaagcatgc aaattcaata tattgcagag ttaacgtagg cctgataagc gtagcgcatc
     8941 aggcagtttt gcgtttgttc atcagcctga tgccgggcat agcccggcat aaaagattac
     9001 tgcgcagcgg caggtttacg cggacggcgg cgacgttgtt gttcgcctgc cggtttggca
     9061 tcgccgaggc ggcgagacgg tttttctgca ggtttcgcgc ttgcagattt tgcgccacct
     9121 tccccacggc gtggttgctg ttgaccgcga ccaccacctt gcccacgacc gccgccgcca
     9181 cgttgctggc gaccgttctg gatcggttcg gctttgattg acgggtccgg ctcatagccc
     9241 ggaatcgcaa tgcgcgggat ctcttttttc agcagttttt cgatatcacg cagcagtttg
     9301 tgttcatcaa cacacaccag cgacaacgct tcaccggtag cagccgcacg accggtacgc
     9361 ccgatacggt ggacataatc ttccggtacg tttggcagtt catagttgac gacgtgcggc
     9421 aactcttcga tatccagacc gcgcgcagcg atatcggtcg ccaccagtac gcgaatgtca
     9481 ctcgatttaa aatcagccag cgcacgggta cgcgcacctt gcgatttatt cccgtggatc
     9541 gccgcactac ggatgccatc tttattgagc tgctcagcca gatggttagc gccgtgtttg
     9601 gtacgggtaa acaccagcac ctgctgccag ttccctttac caatcatgtg cgacagcaat
     9661 tcgcgtttgc gtttcttatc gacaaagtga acgtgctggg tcacctgatc agacgcggta
     9721 ttgcggcgtg ccacttcgat ttccagcggg ttgtgcaaca gtttttccgc cagggcttta
     9781 atatcgtcag agaaggtcgc agagaacagc aggttctggc gtttcgctgg cagttttgtt
     9841 aacacgcgac ggatatcgtg gataaagccc atgtcgagca tgcggtcagc ttcatcgagg
     9901 acgaggattt caacctgatc cagcttcact gcattttgat gttccaggtc cagcaaacgt
     9961 cccggtgttg ccaccagaac atcaacgccg ccgcgcagct tcatcatttg cgggttaatg
    10021 ctgacaccgc caaacaccac cagcgaacga atgttcaaat atttgctgta atcacggacg
    10081 ttttcgccaa tctgcgcagc cagttcacgg gtcggggtaa gaatgagcgc acgtaccgga
    10141 cgacgccctt tggcgtgcgg ctggcgagtg atcaggtgtt gcaacagcgg cagcgtaaag
    10201 cccgctgttt taccggtacc ggtctgggcg ctagccatca ggtcgcggcc ttccagcacc
    10261 gcagggatcg cctgctgctg aataggggtg ggttcacggt aaccctgctc ggcaacggcg
    10321 cgcaggatat cagggcttaa acccaaagaa tcgaaagaca taactactcc gaaccgcccc
    10381 gaccgttacc ggtgtagttt tcagggagat actgaaaagg aaaatgacaa aaaccacaat
    10441 gtcatgaggg agcggagtgt agcagttttt gtgacgcagc gcataaatta tccctgtgac
    10501 tggcgttgct aaaattctta gccatactgg acaactcccc tatggagtca taatcttaat
    10561 caatcatttg attaagaatg gactgtgcga tgaataatcc tgccatgaca atcaagggtg
    10621 aacaggcgaa aaaacagctg attgctgccg cactggcgca gtttggtgaa tatggaatga
    10681 acaccaccac tcgcgagata gccgcccagg ccgggcagaa tatcgctgcc atcacctact
    10741 acttcggttc gaaagaagat ttgtacctcg cctgcgccca gtggattgcc gattttattg
    10801 gcgagcagtt ccgtccgcat gccgaggaag ccgaacgctt gttcgcgcaa ccacagcctg
    10861 atcgggctgc catccgtgaa ctgatccttc gcgcctgcag gaacatgatt aagctgctca
    10921 cccaggatga caccgtcaac ctcagcaagt ttatctcccg tgagcagctc tctcccacgg
    10981 cagcctacca cctggtgcac gaacaggtga ttagtccgct acacagccac ctgacacgcc
    11041 tgattgccgc ctggaccggc tgcgacgcca atgacacccg catgatcctt catacccatg
    11101 cgttgattgg tgagattctg gcgttccgtc ttggcaaaga aacaattctg ttacgtaccg
    11161 gctggaccgc gttcgatgaa gaaaagaccg aactgatcaa ccagacggtg acctgtcata
    11221 tcgacctgat tttgcaagga ttatcgcaaa ggagtttgta gtgatgaaaa aacctgtcgt
    11281 gatcggactg gcggtagtgg tacttgccgc cgtggttgcc ggaggctact ggtggtatca
    11341 aagccgccag gataacggcc tgaagctgta tggcaacgtg gatattcgta cggtgaatct
    11401 tagtttccgt gttggggggc gcgttgaatc gctggcggtg gacgaaggtg atgcgatcaa
    11461 agcgggccag gtgctgggcg aactggatca caagccgtat gagattgccc tgatgcaggt
    11521 gaaagcgggc gtctcggtgg cacaggcgca gtatgacctg atgcttgccg ggtatcgcga
    11581 tgaagaaatc gctcaggccg ccgcagcggt gaaacaggcg caagccgcct atgactatgc
    11641 gcagaacttc tataaccgcc agcaaggact gtggaaaagc cgcaccattt cggcaaatga
    11701 cctggaaaat gcccgctcct cgcgcgacca ggcacaggca acgctgaaat cagcacagga
    11761 taaattgcgt cagtaccgtt ccggtaaccg tgaacaggac atcgctcagg cgaaagccag
    11821 cctcgaacag gcgcaggcgc aactggcgca ggcggagctg aatttacagg actcaacgtt
    11881 gatagcaccg tctgatggca cactgttaac gcgcgcggtg gagccaggca cggttctcaa
    11941 tgaaggtggc acggtgttta ccgtttcact aacgcgtccg gtgtgggtgc gcgcttatgt
    12001 tgatgaacgg aatcttgacc aggcccagcc ggggcgcaaa gtgctgcttt ataccgatgg
    12061 tcgcccggac aagccgtatc acgggcagat tggtttcgtt tcgccgactg ctgaatttac
    12121 cccgaaaacc gtcgaaacac cggatctgcg taccgacctc gtctatcgcc tgcgtattgt
    12181 ggtgaccgac gccgatgatg cgttacgcca gggaatgccg gtgacggtgc aattcggtaa
    12241 cgaggcagga catgaatgat gccgttatca cgctgaacgg cctggaaaaa cgctttccgg
    12301 gcatgggcaa gcccgccgtc gcgccgctcg attgtaccat tcacgccggt tatgtgacgg
    12361 ggctggtggg gccggatggt gcaggtaaaa ccacgctaat gcggatgctg gcgggattac
    12421 tgaaacccga cagcggcaat gccacgatga ttggctttga tccaatcaaa aacgacggcg
    12481 cgctgcacgc cgtgctcggc tatatgccgc agaaatttgg tctgtatgaa gatctcacgg
    12541 tgatggagaa cctcaatctg tacgcggatt tgcgcagcgt caccggcgag gcacgtaagc
    12601 aaacttttgc tcgcctgctg gagtttacgt ctcttgggcc gtttaccgga cgcctggcgg
    12661 gcaagctctc cggtgggatg aaacaaaaac tcggtctggc ctgtaccctg gtgggcgaac
    12721 cgaaagtgtt gctgctcgat gaacccggcg tcggcgttga ccctatctca cggcgcgaac
    12781 tgtggcagat ggtgcatgag ctggcaagcg aagggatgtt aatcctctgg agtacctcgt
    12841 atctcgacga agccgagcag tgccgtgacg tgttactgat gaatgaaggc gagttgctgt
    12901 atcaggggga accaaaagcc ctgacacaaa ccatggccgg acgcagcttt ctgatgacca
    12961 gtccacacga gggcaaccgc aaactgttgc aacgcgcctt gaaactgcca caggtcagcg
    13021 acggcatgat tcaggggaaa tcggtacgtc tgatcctcaa aaaagaggcc acaccagacg
    13081 atattcgcca tgccgacggg atgccggaaa tcaacatcaa cgaaactacg ccgcgttttg
    13141 aagatgcatt tattgatttg ctgggtggtg ccggaacctc agaatcgccg ctgggtgcaa
    13201 tattgcatac ggtagaaggc acacccggcg agacggtgat cgaagcgaaa gaactgacca
    13261 agaaatttgg tgattttgcc gccaccgatc acgtcaactt tgccgttaaa cgtggggaga
    13321 tttttggttt gctggggcca aacggcgcgg gtaaatcgac cacctttaag atgatgtgcg
    13381 gtttgctggt gccgacttcc ggccaggcgc tggtgctggg gatggatctg aaagagagtt
    13441 ccggtaaagc gcgccagcat ctcggctata tggcgcaaaa attttcgctc tacggcaacc
    13501 tgacggtcga acagaattta cgctttttct ctggtgtgta tggcttacgc ggtcgggcgc
    13561 agaacgaaaa aatctcccgt atgagcgagg catttggcct gaaaagtatc gcctcccacg
    13621 cgaccgatga actgccatta ggttttaaac agcggctggc gctggcctgt tcgctgatgc
    13681 atgaaccgga cattctgttt ctcgacgaac caacgtccgg cgttgatccc ctcacccgcc
    13741 gcgaattttg gctgcatatc aacagcatgg tagagaaagg cgtcacggtg atggtcacca
    13801 ctcactttat ggatgaagca gaatattgcg accgcatcgg cctggtgtac cgtgggaaat
    13861 taattgccag cggcacgccg gatgatttga aagcgcagtc ggccaacgac gagcaacccg
    13921 atcccaccat ggagcaagcc tttattcagt tgatccacga ctgggataag gagcatagca
    13981 atgagtaacc cgatcctgtc ctggcgtcgc gtacgggcgc tgtgcgttaa agagacgcgg
    14041 cagatcgttc gcgatccgag tagctggctg attgcggtag tgatcccgct gctactgctg
    14101 tttatttttg gttacggcat taacctcgac tccagcaagc tgcgggtcgg gattttactg
    14161 gaacagcgta gcgaagcggc gctggatttc acccacacca tgaccggttc gccctacatc
    14221 gacgccacca tcagcgataa ccgtcaggaa ctgatcgcca aaatgcaggc ggggaaaatt
    14281 cgcggtctgg tggttattcc ggtggatttt gcggaacaga tggagcgcgc caacgccacc
    14341 gcaccgattc aggtgatcac cgacggcagt gagccgaata ccgctaactt tgtacagggg
    14401 tatgtcgaag ggatctggca gatctggcaa atgcagcgag cggaggacaa cgggcagact
    14461 tttgaaccgc ttattgatgt acaaacccgc tactggttta acccggcggc gattagccag
    14521 cacttcatta tccccggtgc ggtgaccatt atcatgacgg tcatcggcgc gattctcacc
    14581 tcgctggtgg tggcgcgaga atgggaacgc ggcaccatgg aggctctgct ctctacggag
    14641 attacccgca cggaactgct gctgtgtaag ctgatccctt attactttct cgggatgctg
    14701 gcgatgttgc tgtgtatgct ggtgtcagtg tttattctcg gcgtgccgta tcgcgggtcg
    14761 ctgctgattc tgttttttat ctccagcctg tttttactca gtaccctggg gatggggctg
    14821 ctgatttcca cgattacccg caaccagttc aatgccgctc aggtcgccct gaacgccgct
    14881 tttctgccgt cgattatgct ttccggcttt atttttcaga tcgacagtat gcccgcggtg
    14941 atccgcgcgg tgacgtacat tattcccgct cgttatttcg tcagcaccct gcaaagcctg
    15001 ttcctcgccg ggaatattcc agtggtgctg gtggtaaacg tgctgttttt gatcgcttcg
    15061 gcggtgatgt ttatcggcct gacgtggctg aaaaccaaac gtcggctgga ttagggagaa
    15121 gagcatgttt catcgcttat ggacgttaat ccgcaaagag ttgcagtcgc tgctgcgcga
    15181 accgcaaacc cgcgcgattc tgattttacc tgtgcttatt caggtgattc tgttcccgtt
    15241 cgccgccacg ctggaagtga ctaacgccac catcgccatc tacgatgaag ataacggcga
    15301 gcattcggtg gagctgaccc aacgttttgc ccgcgccagc gcctttactc atgtgctgct
    15361 gctgaaaagc ccacaggaga tccgcccaac catcgacaca caaaaggcgt tactcctggt
    15421 gcgtttcccg gctgacttct cgcgcaaact ggataccttc cagaccgcgc cgttgcagtt
    15481 gatcctcgac gggcgtaact ccaacagtgc gcaaattgcc accaactacc tgcaacagat
    15541 cgtcaaaaat tatcagcagg aactgctgga aggaaaaccg aaacctaaca acagcgagct
    15601 ggtggtacgc aactggtata acccgaatct cgactacaaa tggtttgtgg tgccgtcact
    15661 gatcgccatg atcaccacta tcggcgtaat gatcgtcact tcactttccg tcgcccgcga
    15721 acgtgaacaa ggtacgctcg atcagctact ggtttcgccg ctcaccacct ggcagatctt
    15781 catcggcaaa gccgtaccgg cgttaattgt cgccaccttc caggccacca ttgtgctggc
    15841 gattggtatc tgggcgtatc aaatcccctt cgccggatcg ctggcgctgt tctactttac
    15901 gatggtgatt tacggtttat cgctggtggg attcggtctg ttgatttcat cactctgctc
    15961 gacacaacag caggcgttta tcggcgtgtt tgtctttatg atgcccgcca ttctcctttc
    16021 cggttacgtt tcgccggtgg aaaacatgcc agtatggctg caaaacctga cgtggattaa
    16081 ccctattcgc cactttacgg acattaccaa gcagatttat ttgaaggatg cgagtctgga
    16141 tattgtgtgg aatagtttgt ggccgctact ggtgataacg gccacgacag ggtcagcggc
    16201 gtacgcgatg tttagacgta aggtgatgta acttcttatc tttcgccagc aaagacacta
    16261 ccgccgggcc ggcaaggatt gccagccctg caattgccag caaaaagttg ttttccagta
    16321 cgcgggacaa cagccacagc acaatcagcg ctgcgccaaa ataccaggtc gtggtcagct
    16381 cttccaacac gtctgccacc tcacgccagc gttgctcggg gtggcgctga aacaccaaca
    16441 tcatcactac cgtgacacaa tacagtgcgc ataatcccac gccgacgtga accagagtct
    16501 tactcatcca gcccaccacc agcagcgcca ctaccagtaa atgcaacata atctgccagc
    16561 aacttagacc cgttgcgaca cgaacacgtt gttgccactt catggcttct ctcctgaaga
    16621 atttatctct acttactcag agtaccgaac tttacggaaa attccgtaag accgcacctt
    16681 tttttgacgc cgactacact atttcaatga gataagaggc aaagacagga aaattatgcc
    16741 cgatcaaaca caacaatttt cgttcaaggt gctcaccatc aacattcaaa aaggttttac
    16801 cgcgtttaac cgacgcttca ttttgccgga acttcgcgac gcggtgcgta ccgtcagcgc
    16861 cgatattgtt tgcctgcagg aagtgatggg cgcgcacgaa gttcatccgc tgcatgtgga
    16921 aaactggccc gatacctcgc actacgagtt tctcgccgac actatgtgga gcgattttgc
    16981 ctacggtcgc aatgccgtat acccggaagg gcatcacggc aacgccgtac tgtcgcgtta
    17041 tcccattgaa cattatgaga atcgcgatgt ttcggtcgat ggtgcggaaa agcgcggcgt
    17101 gctctactgc cgcattgtgc cgccgatgac cggaaaagcg attcatgtga tgtgcgtaca
    17161 tctgggcctg cgtgaggcgc accgtcaggc gcagcttgcg atggggtgaa tgagctaccg
    17221 gacggcgaac cggtattggt ggcgggtgat ttcaatgact ggcggcaaaa agctaatcat
    17281 ccgttaaaag tgcaggccgg actggatgag atttttaccc gcgcccacgg acgcccggcg
    17341 cgcacgtttc cggtgcaatt tcctctacta cgactggaca ggatctacgt caaaaatgcc
    17401 agcgccagcg cgccaaccgc gttgccgctg cggacatggc gacacctttc tgatcatgcc
    17461 cctttaagtg cggagattca tttatgaaat gtagctggcg cgaaggcaat aagatccagt
    17521 tgctggaaaa cggcgagcaa tattatcccg cggtgtttaa ggcgattggc gaggcacaag
    17581 aacgcatcat tcttgaaacg tttatctggt ttgaggataa cgtcggcaaa cagctgcatg
    17641 cggcactact ggcagcagcg caacgcgggg ttaaagcgga agtcttgctg gatggctacg
    17701 gttcgccgga tctcagcgat gagtttgtca ataaactgac ggcagctggc gtagtgttcc
    17761 gctactacga tccccgccct cgcctttttg gtatgcgcac caatgtgttt cgccggatgc
    17821 atcgcaaaat tgtggtgatc gacgcgcgta tcgcctttat tggcgggctg aattactccg
    17881 ccgagcatat gtccagctac ggtccagagg ctaaacagga ttacgcggta cgccttgaag
    17941 ggccgattgt cgaagatatt ctccagtttg agctggaaaa cctgcctgga cagagcgcgg
    18001 cacggcgctg gtggcgacgt catcacaaag cggaagagaa ccgccagccg ggagaagcgc
    18061 aggtattgct ggtctggcgc gataacgaag aacatcgcga tgatattgaa cgccattatc
    18121 tgaaaatgct cactcaggcg cggcgggaag tgattatcgc caacgcctac ttcttccccg
    18181 gctatcgatt tttacacgcc ttgcgtaaag cggcacggcg cggggtgcgg atcaaactga
    18241 tcattcaggg cgaaccggat atgccgattg tcagagtcgg tgcgcgcttg ctgtataact
    18301 atctggttaa aggcggcgtt caggtttttg agtaccgccg ccgcccgctc cacggcaaag
    18361 tggcattgat ggacgatcac tgggcgacag tagggtccag taatctcgat ccgctcagtt
    18421 tgtcactgaa tctcgaagca aatgtcatca tccacgatcg tcattttaac cagacgctgc
    18481 gcgataatct gaacggcatt attgccgcag attgtcagca ggtggatgaa accatgctgc
    18541 ccaaacgcac ctggtggaac ctgaccaaaa gcgtgctggc gttccacttt ttacgccact
    18601 tcccggcgct ggttggctgg catccggcac acacaccacg tctgacgcag gttgatcccc
    18661 ccgcacaacc gacaatggaa acgcaggatc gggtagaaac tgaaaacacg ggggtaaaac
    18721 cctgatgagt aaatcacacc cgcgctagcg cttagcaaag aagatcctca cctggctgtt
    18781 ttttatcgcg gtgattgtgt tgctggtggt ctacgccaaa aaagtggact gggaagaggt
    18841 ctggaaggtc atccgcgact acaatcgcgt tgcgctgctt agtgcggtcg ggctggtggt
    18901 cgtcagctat ctgatttacg gctgctatga cctgctcgcc cgcttttact gcggtcacaa
    18961 actggcgaag cgccaggtga tgctggtgtc gtttatctgc tacgccttca acctgacgct
    19021 cagtacctgg gtcggcggca ttggtatgcg ctatcgtttg tactcccgac tagggttacc
    19081 gggcagcact attacgcgga ttttctcgct cagtattacc accaactggc tgggctatat
    19141 tttactggca gggattatct ttaccgcagg cgtggtggtg ttgccggatc actggtatgt
    19201 cgctcaaact acgctgcgta ttctcggcat tggcttactg atgattatcg cggtttattt
    19261 gtggttttgc gctttcgcga agcaccgcca tatgaccatc aaagggcaaa agctggtgct
    19321 gccttcatgg aaattcgccc tcactcagat gctgatttcc agcgttaact ggatggtaat
    19381 gggggcgatt atctggctgt tacttggtca aagcgtgaac tatttctttg tactgggcgt
    19441 gttactggtt agtagtattg ctggcgtcat cgtgcatatt ccggcgggga tcggtgtgct
    19501 ggaagcggtg tttatcgcac tactggctgg ggagcatacc tcgaaaggga gcattatcgc
    19561 cgcgctgctc gcttatcgcg tcctgtatta ctttatcccg ctgctgctgg cgctgatctg
    19621 ttacctgcta ctggaaagcc aggcgaagaa gctacgggcg aaaaatgaag cggcgatgtg
    19681 aaatttagtt aagttcttta gtatctgcat tcacgactaa tgaaaaaaac gcgtatgcct
    19741 ttgccagaca agcgttatag ctatcgcggc aagtatccca aagaagccga taaaatagta
    19801 atagagtgtc aatgcacctg taaaagcgca acgaaccgga ctccacaact cttcactatg
    19861 gcattttcct gccagttcaa ggagcttatt tcgtccatgc gagataatgc ctggccataa
    19921 cacaatcact aagcaactta ttacccaaat gggccgttca ctcattaatg ccgcattcac
    19981 gatgattacc agggccaacc ctgtcaacgt catcataatg ataaaacggt gagaaccagg
    20041 atcaacatta aataataaac cggccagcat actgattaaa aacatcgcgc ccgcagtgct
    20101 gaacacaatg ataatggcat atacaggaac taaacttgca acgacaatcg ccaccaaaga
    20161 gagtaaaaac agatatatta cgcctgcgat gacggcattt atcttgttga tatccttact
    20221 taagccatac atacaaacta ataataaccc tatggcgaca tacattgttg gtgaatcata
    20281 tttggttgcc gtaatttcga gatacttagc tccccaggcc agtcctgcgg ttgccaccaa
    20341 acctaaagcg gtaatgagcc atataccgtt taatatcctg gtctcgtgct gaaaatctaa
    20401 tttattgctg ttttgtgagt atgattccat tcttttagtt gtccttagaa aaaatattaa
    20461 tattcaccat caataaattt aaacccacgc atacaaaata cttttgtatt tatggatacg
    20521 gaaaacgctt ccgctcggcc ataaggcgta ttttatacct ggtacttgtt aagtgtcgtt
    20581 tttagtaaga aaagaaatcc cgacacatgg ccgggatatt gatggtgaaa aagaattaac
    20641 ggcggttgcc aaaaattcgc agcaacatca ggaacaggtt gatgaagtcc agatacaagg
    20701 ttaacgcgcc aagaatggaa tatttgcgca ggttcgacgt gtcgcgggta tcaatctgct
    20761 cacccatatt tttcaatttc tgagtgtcat acgccgtcaa tccgacaaag acaatcacgc
    20821 cgatgtaggt aactgcccac atcaatgctt cgcttttcag ccagaagttg accagcgatg
    20881 ccagcacaat gccgattaac gccataaaca gcatattgcc aaagccactt aaatcgcgct
    20941 tcgtggtgta accgtacagg ctcattgcgc cgaacatccc ggcagtaacg acgaaagtac
    21001 tggcgataga agcagcggta tagacaatga atatactgga aagcgtaaga cccgtcagcg
    21061 ccgaataaag cataaagagc atcgtcgtta cacctgcgct cagcttttga atcatcgctg
    21121 ataacacaat aaccaatgct aattgcgcga tgatcagacc gattaaaaag acacggttag
    21181 tgaacaacag ctccatcacg gccgcggaat tagccgcata ccaggcaaca aatgcggtca
    21241 gcaacaagcc aacggtcatc cagccataga cttgcgccat ataagtttgc aagccagccc
    21301 ggggttgtac gattgaatca gaacgtggga atctgtccat gacgatctcc tgaagatata
    21361 aggaatatct taaggatact gcaaaatgat gaggctgtgc atcgacgcag cgtaaacgca
    21421 tgtactgagc ggtgaaattg ccggacgcag cggtgcctta tccggctaac aaaaaactac
    21481 cagcgttttg ccgcctgctg atcgctctcc cgagcttcaa cccagcggtc gccttccggc
    21541 gtggcttcgc gcttccagaa cggtgcgcgg gttttgagat aatccataat aaactgcccg
    21601 gcttcaaacg cactgctgcg atgcgcactg gtgacaccgg caaaaacgat ttcatcgccc
    21661 ggccataatt ccccgatgcg gtgaatcaca gtgacgcgcc ccagcggcca gcggttacgc
    21721 gcttcatcaa caatttctgc cagtgctttt tcagtcattc ccggatagtg ttcgagggtt
    21781 aatgctttga cgctgtcgcc caggttatgg ttgcgcacct taccagtaaa ggtgactacc
    21841 gcaccgtctt cgtcacgctc cgccagccac gggtactctt ctcctacgct gaacggctgc
    21901 ggaccaacaa caattttggt ttctgccatc ttaacctccg gttaccggcg ggaagaaagc
    21961 tacttcgtcg ccgtcagtca gcggatggtc aaaactcacc agcgtctggt tgacggcagc
    22021 cagtaatttg ccatcttcca gcgccagcgc ccagcgatcg ctctgcgcag ccatgtgctg
    22081 gcgtaacgct tcaacagttg ggaaatccgc agccacttcg gttgcatctg ttcccaccaa
    22141 ctcgcgcacc tgcgcgaaaa aaagaacttt aatcatccgc ttccacctta aagtcacccg
    22201 acttgccgcc gctcttcgcc agcaaacgta ccggaccaat caccatatct ttttgcaccg
    22261 ctttgcacat gtcataaatg gtcagcgccg ccacggaggc cgcggttaat gcttccattt
    22321 cgacaccggt tttcccggtc aggcggcata aggtttctat acgcacccga ttgtgctccg
    22381 gctcggcctg taaattgact tcaactttgc tgagcatcag cggatgacag agcgggatca
    22441 gatcccaggt gcgttttgcc gcctgaatac cggcaatacg cgcagtggca aatacgtcgc
    22501 ctttgtggtg gcgaccatca ataatcatcg ccagcgtctc gctgcgcatg gtgacaaagg
    22561 cttccgcccg cgcttcacgc acggtttccg ctttggcgga gacatccacc atgtgcgctt
    22621 cgccagcggc gttgatatgg gtcagttgcg acatacttat ttcttcaaat gtggatggaa
    22681 attacacgga cgcgtacggg catccagctg cggcgcgatg atattttccc atgcggtacg
    22741 gcacgctttg gtcgaacccg gcatggcgaa aatcagcgtt ttgttggcga cgcccgcaac
    22801 cgcacgagat tgcaacgtgg aagtgccaat ctcttcaaac gacaacatcc ggaacacttc
    22861 accaaaacct tcaacttcac ggtcgaacaa cggcagcaat gcttcgggag cctgatcacc
    22921 ttccgtcagg ccagtaccac ccgtaatcaa taccacttgt acatcgtcgc tggcgatcca
    22981 cgcagatacc tgagcgcgaa tagcgtagcg gttttctttc acaatggctt tatcgacaac
    23041 gtgatggccc gcttcttgcg ccgaatcgcg cagatagtga ccggaggtat cgtcttcttc
    23101 accgcgacga ttagaaaccg taagaatagc aatacgggtc gggataaatt cagtgcttac
    23161 ctgactcatc tgatctctcc ttttgacgtt ttagccgcca atgtacgata agttttgcgt
    23221 aataccggtg ttgttttgat gcaggaaatg ggtctgtttc ttctcccgca gcgccgctga
    23281 aatacgcgct tccagcgcct gttgctgggt atcgtcttcc agcagatcgc gcaggttaac
    23341 gccgccttca ccaaacaggc agagatggag tttaccaatg gaggaaacgc gcaggcggtt
    23401 gcaagtggcg cagaagtctt tttcatacgg catgataagg ccaatctctc cggcgtaatc
    23461 tggatggcaa aagacttgcg cgggaccgtc gctgcgttga cgtaattggt ggatccagcc
    23521 gcgacgcagt agctcgtcac gcagaacctg accagagatg tgatgcttac ggaagagctc
    23581 gctgccctcg cccgtttcca tcagttcgat gaaacgcagc tggatagggc gatgctggat
    23641 ccagttcaga aaggtgtcga gctggtgatg attaacatca cgcatcagca cggtattgac
    23701 tttgaccttc tcaaaaccgg cctcaaatgc agcatcaatc cctgccatga cctggttgaa
    23761 tttatcctgc ccggtaatag cgtgaaactg gcgggcgtcc agactgtcga cactgacgtt
    23821 aatgccagta agtcccgcat cgcgccagtt cgccacatcg cgttccagac ggtaaccatt
    23881 ggtggtgacc gcaatctggc ggatagcgtc gttttcccgc acagcggcga tgatatcggt
    23941 aaagtcgcgg cgtaaagacg gctcacctcc tgtcaggcgc actttttcgg tgcccagact
    24001 ggcgaaggcg cgcgtaaccc ggcgaatttc atcgacggta agaaagcctt tattggtgac
    24061 gccgctcggt ttgtagccat ccggcaggca gtaggtgcaa cgaaagttac acacatcggt
    24121 aatcgacagg cgcaagtagt aaaacttacg cgcaaatgca tcagtcagtt gtgaagccat
    24181 gtacaccttt ccagatacgg gaggcgaagt catttcttcc ttcgccctgg tggcaatctt
    24241 tacgagcatt gtcacggcca aagcaccgta tcagttgacc caggtgcaga ggctagagtg
    24301 tttagtggtt aagccgatac tagcgtgtaa atgagatttt taccatccac actttcgcta
    24361 tgcaatcatg tatatagcgt catgatcgtg ctatttcgct acagaataag ggaaaatggc
    24421 agttttactt tgatatacat catcagggag gcatagcgtg catcgtcctt tagaggtaga
    24481 agaaaatagt ttgtatcctc aatattggca ggttaattgc tgtttccccg caatttgcgc
    24541 tactgtagcg cggataatct gactccagga aactatatgc gcaatcgtac gctggctgat
    24601 cttgatcgtg tcgttgctct cggcggaggg catggactgg gacgcgttct ctcatcactt
    24661 tcgtctttgg gttctcgttt aacgggtatc gtcaccacca cggataatgg tggctcgacg
    24721 gggcgtattc gccgttcaga aggcggcatt gcctggggcg atatgcgcaa ctgcctcaac
    24781 caactgataa cggaaccgag cgtcgcctcc gcgatgtttg aataccgttt tggcggcaat
    24841 ggcgaacttt ccgggcacaa ccttggaaac ttgatgttaa aggcgctgga tcaccttagc
    24901 gtgcggcctc tggaagccat caatttaatt cgtaatctgc tgaaagtgga tacgcatttg
    24961 attccaatgt cagagcatcc cgtcgatctg atggcgattg acgatcaggg gcatgaagtt
    25021 tacggcgagg tcaatatcga ccagttaact acgccgattc aagagttatt attaacgccg
    25081 aatgtacccg caacgcgtga ggcagttcac gctatcaatg aagcggatct catcattatt
    25141 gggcctggca gtttttatac cagcctgatg ccaattctgc tgctgaagga aatcgcccag
    25201 gcattacgcc gcacgccagc gccgatggtt tatatcggca atctggggcg tgagttgagt
    25261 ttacctgcgg ctaatttgaa gctggaaagc aagctggcaa ttatggagca gtatgttggt
    25321 aaaaaagtca ttgatgcggt catcgtcggg ccaaaagtgg atgtctcggc ggtgaaagag
    25381 cggattgtga tccaggaggt actggaggcc agcgatattc cgtatcgtca tgaccgccag
    25441 ttgttacata acgcgctgga aaaggcgtta caggctttag gttaacggaa atccgaagga
    25501 aaattccggc ttcctattga agacaaagtg cgcgttgttt atgccggatg cggcgtgaac
    25561 gccttatccg gcctacaaac cgcgcaaatt caatatattg cggagaaaat gtaggcctga
    25621 taagcgtagc gcatcaggct gttttccgtt tgtcatcagt cttcttcgct atcctgttac
    25681 gatgcggcaa taaacaactc acgcaactga tgcaactggt cacgaatttg tgccgcttct
    25741 tcgaactcca gattctgcgc gtgttgcatc atcagccctt ccagttcatg gattttctgc
    25801 tgcaacgctt tcggcgacat atccatcggc acattatccg gctcaacaat cgggcgcgat
    25861 tttcctctgc ccttcgcttt ggttttggca atgttctgcc ccagcgccag gatatcgacc
    25921 actttcttgt tcaagccttg cggcgtaatg ccgtgttcct cgttgtactt ctgctgtttc
    25981 tcacggcgac gttcggtttc gccaatcgct ttcgccattg atggggtgat cttatcgccg
    26041 tagagaatcg ctttaccgtt aacgttacgt gccgcacgac caatggtctg gatcaacgaa
    26101 cgttcggaac gcaggaagcc ttctttgtca gcgtcgagga tcgccaccag cgacacttcc
    26161 ggcatatcca gaccttcgcg cagtaagttg atccctacca gcacgtcgaa ctcacccaga
    26221 cgcaagtcgc ggataatctc catacgttcg acggtgtcga tatctgagtg aagataacgc
    26281 acgcgctcgc catgttcttc gagatattcg gtaagatctt ccgccatccg cttggtcagt
    26341 gtggtgacca gtacgcgttc gttaattgcc gctcgctgac gaatctccga aagaagatca
    26401 tcaacctgtg tcgccaccgg ccgcacttcg ataatcgggt caagcaatcc ggttggacgc
    26461 accacctgat ccaccacatc gccgccggat ttttccagct cgtaattacc cggcgtcgcc
    26521 gaaacataga tggtttgcgg cgctaatgct tcgaactctt caaacttaag cggacggtta
    26581 tccagcgctg atggcaggcg gaagccgtac tccaccagtg tctctttacg cgcccggtca
    26641 ccgcgataca tgccgccaat ttgtggaatg gtgacgtgag attcatcgac gaccagcagc
    26701 ccatcggcag gcaggtaatc aaacagcgtc ggcggtggct cacccggtcc acgaccggag
    26761 aggaagcgcg agtagttttc aatccccgaa cagtagccca gctcgttcat catctccaga
    26821 tcaaactggg tacgctgggt cagccgctgc tcttccagca gtttgttgtt ttccaacagc
    26881 actttgcgtc tggcggccag ttcttctttg atctcctcca tcgcctgtac gatgcgctcg
    26941 cgcggtgtga cgtagtgcgt tttcgggtag atggtaaaac gtggaatagt ggaaacaatc
    27001 tgcccggtca gcgggtcaaa taacgacaat cgttccactt cctcgtcaaa cagttccacg
    27061 cgaagtgcaa tgtcatccga ttctgccggg aagatatcaa tcacctcgcc acgaacgcgg
    27121 aaagtaccac gctggaatgc ttgatcatta cgagcgtatt gcagctccgc cagtcggcgc
    27181 agaatcgcgc gctgatcgat aatcataccg accgtgagat ggagcatcat cttgagatat
    27241 aaatcaggat cgcccagacc ataaatcgcg gaaacagagg ccactacaac cacatcacgc
    27301 cgctccagca tcgctttggt ggcggataaa cgcatctgct caatatgttc gttaaccgag
    27361 gcatctttct caatgaaagt gtcggaactc ggtacatagg cttccggctg atagtagtcg
    27421 tagtaggaga cgaaatactc caccgcgttt tccgggaaga actctttcat ttcgccatac
    27481 agctgggccg ccagcgtttt gttgggcgca agtaccatgg ttgggcgctg aaggtcagca
    27541 atgacattgg caatggtgaa ggttttccct gagccagtca cgccaagtaa cgtctggtgc
    27601 gccaggccat cttccagccc ctcttcgaga cgtcgaatcg cctctggctg atcgccagaa
    27661 ggtttaaaag cggaattcag tttgaacggt ttactcatga gtcgctacct gaaggagttg
    27721 ggcgggcagg tatgtaattt tactcgtcgt acttaattat gccaacaaat tatactggat
    27781 aaaaaaacag ttcatcacca taatatttct gatacagcgt aaactccgct gtcaatcatg
    27841 agcaaaattt actctgtggc gagataaaac tccggcctta ccgggttatc cccaaagcaa
    27901 cggctttttt aacatttgtc aagatgagtg atgacagttt tatggcaaga gatgcctgtt
    27961 cagtgactgc cattgcattt atctaaccag ttaaaaaata aaagatattt ctttgagccg
    28021 tctttaacgc caattcgcgt gcaagccgcg tattctctcg cttgcctcgt gttttctaac
    28081 tctaatacac atggttatcc acaggaatag tggataactg tctccagccc ctatcccccg
    28141 ccgcctgggc aattcccaca ttcgccgcat gaggcggaag taaaattttt ttcgtgatta
    28201 ttttagaatt taattggtta aattgcagtc aatcgaagac gcgatctcgc tcgcaattta
    28261 accaaataca gaatggctac aacaaggcaa ggtttatgta ctttccggtt gccgcatttt
    28321 ctggattttc tgcaagccag gggatctctc ccagcagcgg cgcgggaatc atgcgggtga
    28381 gcgtggtcat atattcagcg tgacgttttc ccggaggcgt aacatcgttc gccacccaac
    28441 ccgccagagt cagtccggcg tgttgtattg cctgcgcagt caacatcgcg tgattaatac
    28501 agccgagttt cacaccaact accagtatca ccggcagttg ttcctgtgtt acccaatctg
    28561 caaaagtgaa agtgtcagaa agcggcgtaa accagccgcc agcaccttcc actaacaccc
    28621 agtcagcctg ttgttcaagc gcgcgtaatc cggcgctcat taccaatgat tctatcggcc
    28681 tgccctcttg cgcgctgatg atgtgcggcg aagtgggttc tgcgaaggtg taaggattta
    28741 ctgttgcgta atccagctgc aggctgctgt tgtgctgtaa cgccagcgcg tcactattac
    28801 gtaagccttc cggggtcttt tcgctgccag aggcgaccgg tttataacct gccgtccgat
    28861 aacctactgc cttcgcggct tgtaaaagtg cacaactggc gacggttttc cccacttcgg
    28921 tatccgttcc ggtgacaaaa taacgtttac tcacgagcaa tcactcccaa aaaaagatga
    28981 tacgtcagag gatatcgccc ctgttgttgc ggccaggcca gttgcaatcg ctgcaattgc
    29041 gaacgcgtta atattcgcgg gtcgcgccct tcatgaagat gcgtagcacc gatgcctttc
    29101 agcgaacgca tggcactgag cgcatcatca aaccatagtg tgatgggctg aatatgatgt
    29161 tgataatgca cgccgttcag cgactgttcg atttcatctg gcggtaaaaa gcgattagca
    29221 tgcggacgct cgtccaccgc ctgccacgcc tgatgcagtt cgggtaacga tccctgcacc
    29281 agcgtggtaa acgccaccac gcctccgggg cgcaccaccc gatacagctc gcggagtgcc
    29341 gtggataaat taccgcacca ctgcaccgcg agattgctcc atgcaagatc gaacgtcgca
    29401 gtcgctaacg gcagggattc gatatctccc gccagataat ggtctgcagc atctttctgg
    29461 cgggcttgca caagcattgg cggtgagaga tctaacgccg tcacctgtgc gtgacgctct
    29521 cgccagtggc ggctcatcca gcctggccca caccccgcgt ccagtacgtg ggtgtattta
    29581 cgctgtggga gcattgccag taagacgtca gcactccggc gctgtaactc tgcgtgttgc
    29641 tcatagtgcg cggctgcccg accaaatgcc gctgcaatgg cttgtttatt aaccgttgcc
    29701 atgcagcacc tccagcagac ggtcgatatc ctgcatttca tgcgctgcgg ttagcgttaa
    29761 gcgcagtcgc gcagtaccag cgggtacggt tggcgggcga atcgccgtga cccagcagcc
    29821 ttgctggcgc agtttttctg ccagttgtaa cgcacggctg ttatcaccga cgatcaatgg
    29881 ctggatggcg ctgcatgaat cagcaagcgt aaacggcaaa tcctgtactc cggcacgaaa
    29941 acgcgcaatg agtgacacca gtttttcgcg ccgtgcatcc ccctcatcac ggcgaatgac
    30001 cgccagcgac gcacgtaatg cctgcgcctg agcgggcggc atactggtgt tgtagataag
    30061 gtggcgggcg aattgcagca gataatccgc caccgtactg gagcaaagca ccgctgcccc
    30121 gctgacgcca aatcctttgc caaaagtcac caccagcaat tctggtttta ccttttgcag
    30181 ccagcagctg ccgcgcccct gctccccgat aacgcccgtg ccgtgggcat catcgaccat
    30241 caaccagcca ttgtgctgtt gcgttacctg ctggatttcc gccagtggcg cactatcgcc
    30301 gtccatgctg aacacgcctt ctgtcaccac cagttgctgc cccggacagg gggaagcaag
    30361 cagtcgcgcc agatgagtga catcgttatg aacaaaacgg cgaagctgcg acgggcttaa
    30421 actggcagcc tccagcaatg aggcatggct aagccggtca gcgacaatgc ggtcctcttt
    30481 tgccatcatc gcggcaatga ccgcctgatt agcggcaaaa ccagagataa atagcagtgc
    30541 ccgcgaatag cccagccact cggccagttc ttcttccagc gcctgatgcg caacgctata
    30601 accgctgacg tgaccggaac cgccgctacc gacgccaaat tgctccgcac cctgctgcca
    30661 ggcacggata atttgcggat gatggcttaa acctaaataa tcgttactgg aaaagttcag
    30721 atactggcga tcatccgcca ccagccagcg tccggctcct tgcgccactg gataacggcg
    30781 acgcagggca tcggcagcac gccgcgcatc gagcgccgcg ttgattttct cctgccagct
    30841 catagtgctg ccgcgttgta atattcgtca gtatccgggg tcatcagcgc ctgttcaaga
    30901 cgttgctgtt gttcgttatc ccctgccagc acggcagttt gctgcggatt tagccccagt
    30961 ttgcggaaca gttgcaggtc tttatcttct tccggattcg gcgtggtcag cagtttgcaa
    31021 ccgtagaaaa tcgagtttgc gcctgccata aagcacatcg cctgagtctg ttcgttcatc
    31081 tgctcgcgtc cggcagaaag gcgcacgtaa gaggtcggca tcatgatccg cgcgaccgca
    31141 atggtgcgaa taaaatcaaa ggcatcaacg tcatcgttat cggcaagcgg cgtgcctttc
    31201 accttcacca gcatgttgat tggcacgctt tccggcggcg tcggcaggtt tgccagttgc
    31261 agcaataatc cggcgcgatc ttttaccgtt tcgcctaagc ccacaatgcc gccagaacag
    31321 actttgatcc cggcatcgcg cactttttcc agcgtatcga ggcgttcctg ataagtgcgg
    31381 gtggtgatga tattgccgta aaactccggc gaggtgtcga ggttgtggtt gtagtaatcc
    31441 agcccggcgt tcgcgaggcg ctgcgcctga gattcactca acgtgcccag cgtcatacac
    31501 gcctccagcc ctaaatcttt taccccctgc accatctgct ccaggtacgg catatcgcgt
    31561 tcgttgggat tcttccacgc cgcgcccatg caaaaacgcg tcgatcccgc cgctttcgct
    31621 ttgcgcgccg actccagcac ctgttcaact tccatcaacc gctcggcttc cagaccggtt
    31681 ttatagcgcg agctttgcgg gcaatatttg caatcttccg ggcaagcacc ggttttaatc
    31741 gacagcaacg tgctgacctg cacctgacga ggatcgaaat gctggcgatg cacctgctgc
    31801 gcttcaaaca gcagatccag caacggtttt tcaaataatt ctgcgacttg cgacaatgtc
    31861 cagcgtgggc ggtgagccat ggggcttctc caaaacgtgt tttttgttgt taattcggtg
    31921 tagacttgta aacctaaatc ttttcaattt ggtttacaag tcgattatga caaaggacga
    31981 tcttgccttt gaccaacgcc atatctggca cccatacaca tccatgacct ccccgctgcc
    32041 ggtttatccg gtggtgagcg ccgaaggttg cgagctgatt ttgtctgacg gcagacgcct
    32101 gattgacggt atgtcgtcct ggtgggcggc gatccacggc tacaatcacc cgcagcttaa
    32161 tgcggcgatg aagtcgcaaa ttgatgccat gtcgcatgtg atgtttggcg gtatcaccca
    32221 tgcgccagcc attgagctgt gccgcaaact ggtggcgatg acgccgcaac cactggaatg
    32281 cgtttttctc gcggactccg gttccgtagc ggtggaagtg gcgatgaaaa tggcgttgca
    32341 gtactggcaa gccaaaggcg aagtgcgcca gcgttttctg accttccgca atggttatca
    32401 tggcgatacc tttggcgcga tgtcggtgtg cgatccggac aactcaatgc acaacctgtg
    32461 gaaaggctat ctgccagaaa acctgtttgc tcccgccccg caaagccgca tggatggtga
    32521 atgggatgaa cgcgatatgg ttggctttgc ccgcctgatg gcggcgcatc gtcatgaaat
    32581 cgcggcggtg atcattgaac cgattgtcca gggcgcaggc gggatgcgca tgtaccatcc
    32641 ggaatggtta aaacgaatcc gcaaaatgtg cgatcgcgaa ggtatcttgc tgattgccga
    32701 cgagatcgcc accggatttg gtcgtaccgg caaactgttt gcctgtgaat atgcagaaat
    32761 cgcgccggac attttgtgcc tcggtaaagc cttaaccggc ggcacaatga ccctttccgc
    32821 cacactcacc acgcgcgagg ttgcagaaac catcagtaac ggcgaagccg gctgctttat
    32881 gcacgggcca acttttatgg gcaatccgct ggcctgcgcg gctgcaaacg ccagcctggc
    32941 gattctcgaa tctggcgact ggcagcaaca ggtggcggct attgaagtac agctgcgcga
    33001 gcaacttgcc cccgcccgtg atgccgaaat ggttgccgat gtgcgcgtgc tgggggcaat
    33061 cggcgtagtc gaaacaactc gtccggtgaa tatggcggcg ctgcaaaaat tctttgtcga
    33121 acagggggta tggatccggc cttttggcaa actgatttac ctgatgccgc cctatattat
    33181 tctcccgcaa cagttgcagc gtctgaccgc agcggttaac cgtgcggtgc aggatgaaac
    33241 atttttttgc caataacggg aagtcagcgt gtgggtttct ggctacactt tctgcaaact
    33301 agaaaggagg gttcatgaaa ctcatcagta atgatctgcg cgatggcgat aagctgccgc
    33361 atcgtcatgt ctttaacggc atgggttacg atggcgataa tatttcaccg catctggcgt
    33421 gggatgatgt tcctgcggga acgaaaagtt ttgttgtcac ctgctacgac ccggatgcgc
    33481 caaccggctc cggctggtgg cactgggtag ttgttaattt acccgctgat acccgcgtat
    33541 taccgcaagg gtttggctct ggtctggtag caatgccaga cggcgttttg cagacgcgta
    33601 ccgattttgg taaaaccgga tacgatggcg cagcgccgcc gaaaggcgaa acccatcgct
    33661 acatttttac cgttcacgcg ctggatgtag agcatattga tgtcgatgaa ggagccagcg
    33721 gcgcgatggt cgggtttaac gttcatttcc actctctggc aagcgcatcg attaccgcga
    33781 tgtttagtta atcactctgc cagatggcgc aatgctatct ggtatcactt aaaggtatta
    33841 aaaacaactt tttgtctttt taccttcccg tttcgctcaa gttagtataa aaaagcaggc
    33901 ttcaacggat tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt
    33961 tcccgtctgg cgctggcatt ggcttttagc gtgacgctga ccgcctgtag ctcaacacca
    34021 cccgatcaac gtccttctga tcaaactgcg cctggtacct cttctcgccc gattctgtcg
    34081 gcaaaagaag cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgct
    34141 gcagcgtgga atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg
    34201 gcgggcactc aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc
    34261 aagcgtacca acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggtg
    34321 tatgtccctg ctgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat
    34381 gtgaagattg ggctttccct tgatgggggc atgagccctg ccgactggcg tcacgacgtc
    34441 aacccgcgcg gcaaatatat gccaggtaaa ccagcgtggt atatgtacga tagctgccag
    34501 agcaaacgca gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat
    34561 ggcctgcaac tgcaaaacct gaccatcgaa aacacgctgg gcgatagcgt agatgcaggt
    34621 aaccatccgg cggtggcact gcgtactgat ggtgaccagg tacagattaa caacgttaac
    34681 attctcggtc gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa
    34741 acgaatcgtc agccgcgtac gctggtgacc aacagctaca ttgaagggga tgtggatatc
    34801 gtttctggtc gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt
    34861 actcagcaag aagcgtatgt gtttgcaccg gctacgctgt ccaacattta ctacggtttc
    34921 ctcgccgtaa acagccgttt caatgctttc ggtgatggtg tggcgcaact gggccgctcg
    34981 ctggatgttg atgccaatac caacggtcag gtggtgatcc gtgatagcgc catcaacgaa
    35041 ggttttaaca cggctaaacc gtgggccgat gcggtgatct ctaatcgtcc gtttgcgggt
    35101 aataccggca gcgtagatga taacgacgaa atacagcgca atctgaatga cactaactac
    35161 aaccgcatgt gggaatacaa taaccgcggc gtgggtagta aagtggttgc agaggcgaag
    35221 aagtaagagc aattaactat ttgccggatg cggcgtaaac gccttatccg gcctacggtt
    35281 cgatgcgatt tgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgaa
    35341 tgcgacctac attcacatgg cgctttttac atctgacggt ttttattgaa gttaatcaaa
    35401 ctacccgcct tgataatctc gcgctcttca gcagtcagac tttccatata gagcgtaatt
    35461 tccgttaccg gcgcatcttc atggatcaca taacctttaa acgtcgtacc cggattatcc
    35521 agcgccgctt taatgccagg gatgtaaatg taatccccca cttcaaaggt tggcacttcc
    35581 gccatctgca gcggtaacat cccccagttg atgacgttag aacggtagcg tttagtcgcg
    35641 tactcttcgg caatattcgc cagaccgcca atcacacgct ggcagctcgc cgcctgttca
    35701 cgcgcagaac catcgcctgg tttcaccgca tataccatgc tgccaatttc agtttgcagc
    35761 ggatcaatat gctcctgacc agcaatctgc ttaatgcgcg caaacacctc tgtcagctcg
    35821 ctgacattcc ccgccagacg ctgattttcc agctcagccg tcgctttact tctgccaaca
    35881 taaccgggat cgcggcgtga cagggtaaac tccgccagac caatcggatt tgaacgataa
    35941 gaagaggttt caccggaagg aatcagttcg tcggtggtgg tcacttcgtc gaggatcttc
    36001 gagcacactt tcaggacgat attgtcagtc agcgcaccca attccggcca gtctttaatg
    36061 ttcggtccgt aaatcagcgg ttgctgagtt gcccctttca caaagccctg ataaacacgg
    36121 tttttatacg gcgttacatc gaaggcgtac tccggcacgt tgtcccagca atcaagttcg
    36181 ctggcagagg ttaaatagcc accgtttgcc gcagtcgcag cgatagaacg agcgtccatc
    36241 aacgccaccg ctgacatctg cccattagct ggcttagagc cttcgcggtt cgggaagtta
    36301 cgcgtggtgt ggcgaatact taatccgttg ttgattggcg tatcgcccgc gccaaagcat
    36361 gggccgcaga acgcggttct gatgattgcg cctgcgccaa tcaaatctgc taccacacct
    36421 tttttggcta aatccataaa caccggctgt gatgacgggt aaaccgccag cgagaaggtg
    36481 tcattgccac aggattgacc gcgcagtgca ttcgccgccg cgatgacgtt ttcgtagtta
    36541 ccgccagaac agcccgcgat aatcccctgc tgcactttca ggcgaccatt ttccacttta
    36601 tccagcagcg agagtttggc tttaccgtgc gccacgcgtt cggactcaat ttcaatctca
    36661 cgcagaatgt cggtcaggtt ctggttcagt gtgtcgattt catacacgtt gctcgggtgg
    36721 aacggcagcg caatcattgg tttgatggcg cttaaatcaa cgctgatgca gccatcgtag
    36781 tacgccatcg gttgagggtt aagctggcag taatcctggc cgcgaccgtg cagcgccagc
    36841 cagttatgca cttcttcatc agtttgccag acggagctta aacaggtggt ttccgtggtc
    36901 atcacgtcaa cgctgttacg gaaatcggta gagagcgcgc taacgcccgg tccaacgaac
    36961 tccatgactt tgtttttgac gtaaccgttt ttgaacaccg cgccaatgat agccagcgcc
    37021 acatcctgtg gccccacata cggcgcaggt tttccggtca gatgcaccgc aaccacgccc
    37081 ggatagtcga tatcccaggt gtcattaagc agctgtttta ccaactcacc gccgccctca
    37141 ccgactgcca ttgtccctaa tgcaccgtaa cgggtgtggc tgtctgaccc gaggatcatt
    37201 ttgcccccgc ctgccatcat ctcacgcata tattgatgga tgatcgcaat atgcggaagc
    37261 acaaaaatac cgccataacg ctgagctgcc gataaaccaa aaacatggtc atcaccgtta
    37321 atggtgccgc cgacggcgca gagtgagtta tggcagttgg tcagcacata cggcagcggg
    37381 aaacgttcca ttccggacgc tttagcggtc tgtacaatac caacaaaggt aatatcgtga
    37441 gaggctaatg aatcaaactt aattttaagt ttatccatat ttccggacgt attatgagaa
    37501 gagagaatag accaggcaat agtgcctttt ttggcttctt cttttttaat ttcgccggtg
    37561 aaatgttctt cggcaattat ttcgttatta ctggcgagaa acacgccttt
//
LOCUS       DAJTSL010000018        37335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTSL010000018 DAJTSL010000000
VERSION     DAJTSL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.018"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..173
                     /locus_tag="OO433_000818"
     CDS             <1..173
                     /locus_tag="OO433_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6049391.1"
                     /translation="GIVPYELYNSHRDFLNLKEIKPEHPLPSIKLYISYNKSSLNNLV
                     FSRFIDRLNESF"
     gene            320..1837
                     /locus_tag="OO433_000819"
     CDS             320..1837
                     /locus_tag="OO433_000819"
                     /inference="COORDINATES: protein motif:HMM:NF014273.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR6049392.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEDDRIKAINEIRMAIHQ
                     VSPFREEPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDK
                     NAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQIT
                     AMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1838..2987)
                     /gene="ybdL"
                     /locus_tag="OO433_000820"
                     /pseudo
     CDS             complement(1838..2987)
                     /gene="ybdL"
                     /locus_tag="OO433_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            3096..4184
                     /gene="hcxA"
                     /locus_tag="OO433_000821"
     CDS             3096..4184
                     /gene="hcxA"
                     /locus_tag="OO433_000821"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6049393.1"
                     /translation="MPHNPIRVVVGPANYFSHPRSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4194..4391)
                     /locus_tag="OO433_000822"
     CDS             complement(4194..4391)
                     /locus_tag="OO433_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6049394.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4573..6678)
                     /gene="cstA"
                     /locus_tag="OO433_000823"
     CDS             complement(4573..6678)
                     /gene="cstA"
                     /locus_tag="OO433_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6049395.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPKRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6859..7272)
                     /gene="entH"
                     /locus_tag="OO433_000824"
     CDS             complement(6859..7272)
                     /gene="entH"
                     /locus_tag="OO433_000824"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6049396.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7275..8021)
                     /gene="entA"
                     /locus_tag="OO433_000825"
     CDS             complement(7275..8021)
                     /gene="entA"
                     /locus_tag="OO433_000825"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6049397.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8021..8878)
                     /gene="entB"
                     /locus_tag="OO433_000826"
     CDS             complement(8021..8878)
                     /gene="entB"
                     /locus_tag="OO433_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6049398.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFELQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8892..10502)
                     /gene="entE"
                     /locus_tag="OO433_000827"
     CDS             complement(8892..10502)
                     /gene="entE"
                     /locus_tag="OO433_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6049399.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GELQLSYRELNQAADNLACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPADGFTATASPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLSQWLASRASA"
     gene            complement(10512..11687)
                     /gene="entC"
                     /locus_tag="OO433_000828"
     CDS             complement(10512..11687)
                     /gene="entC"
                     /locus_tag="OO433_000828"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR6049400.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKVQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVLASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12062..13018
                     /gene="fepB"
                     /locus_tag="OO433_000829"
     CDS             12062..13018
                     /gene="fepB"
                     /locus_tag="OO433_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6049401.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPTGLNASQSQGKRHDIIQLSGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13022..14272)
                     /gene="entS"
                     /locus_tag="OO433_000830"
     CDS             complement(13022..14272)
                     /gene="entS"
                     /locus_tag="OO433_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6049402.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWLVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAEIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14383..15387
                     /gene="fepD"
                     /locus_tag="OO433_000831"
     CDS             14383..15387
                     /gene="fepD"
                     /locus_tag="OO433_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6049403.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15384..16376
                     /gene="fepG"
                     /locus_tag="OO433_000832"
     CDS             15384..16376
                     /gene="fepG"
                     /locus_tag="OO433_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6049404.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16373..17188
                     /gene="fepC"
                     /locus_tag="OO433_000833"
     CDS             16373..17188
                     /gene="fepC"
                     /locus_tag="OO433_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6049405.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIKHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTHWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLERTAPSTANS"
     gene            complement(17185..18318)
                     /gene="fepE"
                     /locus_tag="OO433_000834"
     CDS             complement(17185..18318)
                     /gene="fepE"
                     /locus_tag="OO433_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6049406.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSSNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18533..22414)
                     /gene="entF"
                     /locus_tag="OO433_000835"
     CDS             complement(18533..22414)
                     /gene="entF"
                     /locus_tag="OO433_000835"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR6049407.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVDGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYLLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPLLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYI
                     DPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22411..22629)
                     /gene="ybdZ"
                     /locus_tag="OO433_000836"
     CDS             complement(22411..22629)
                     /gene="ybdZ"
                     /locus_tag="OO433_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6049408.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(22632..23834)
                     /gene="fes"
                     /locus_tag="OO433_000837"
     CDS             complement(22632..23834)
                     /gene="fes"
                     /locus_tag="OO433_000837"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6049409.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWREPQGSEEYS
                     PIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24077..26317
                     /gene="fepA"
                     /locus_tag="OO433_000838"
     CDS             24077..26317
                     /gene="fepA"
                     /locus_tag="OO433_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6049410.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26492..27112
                     /gene="entD"
                     /locus_tag="OO433_000839"
     CDS             26492..27112
                     /gene="entD"
                     /locus_tag="OO433_000839"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6049411.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27234..27386)
                     /gene="hokE"
                     /locus_tag="OO433_000840"
     CDS             complement(27234..27386)
                     /gene="hokE"
                     /locus_tag="OO433_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6049412.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            27840..28958
                     /locus_tag="OO433_000841"
     CDS             27840..28958
                     /locus_tag="OO433_000841"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6049413.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSILIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQVTVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29024..29272
                     /locus_tag="OO433_000842"
     CDS             29024..29272
                     /locus_tag="OO433_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR6049414.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPTPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29337..29705
                     /locus_tag="OO433_000843"
     CDS             29337..29705
                     /locus_tag="OO433_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6049415.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            29799..30452
                     /gene="nfsB"
                     /locus_tag="OO433_000844"
     CDS             29799..30452
                     /gene="nfsB"
                     /locus_tag="OO433_000844"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6049416.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            30560..31807
                     /gene="ybdG"
                     /locus_tag="OO433_000845"
     CDS             30560..31807
                     /gene="ybdG"
                     /locus_tag="OO433_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6049417.1"
                     /translation="MQDLISQVGDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPAGNDIRSLAGAFKQ"
     gene            complement(31875..33251)
                     /gene="pheP"
                     /locus_tag="OO433_000846"
     CDS             complement(31875..33251)
                     /gene="pheP"
                     /locus_tag="OO433_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6049418.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYICSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33353..36480)
                     /gene="cusA"
                     /locus_tag="OO433_000847"
                     /pseudo
     CDS             complement(33353..36480)
                     /gene="cusA"
                     /locus_tag="OO433_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(36492..>37335)
                     /gene="cusB"
                     /locus_tag="OO433_000848"
     CDS             complement(36492..>37335)
                     /gene="cusB"
                     /locus_tag="OO433_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR6049419.1"
                     /translation="AAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVEAQSEYLLLRET
                     GGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVITAFDLRAGMNI
                     AKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTLTIRKWTLLPGV
                     DAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQGLIDTGSEQRVITID
                     ADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANISGALERMRSESA
                     THAH"
BASE COUNT         8934 a        10440 c         9526 g         8435 t
ORIGIN      
        1 tgggaatagt cccctatgaa ctttataact cccaccgtga ctttcttaat ttaaaagaga
       61 taaaaccaga acatccgtta ccttcaataa agctttacat ttcctataat aaatcttctt
      121 taaataattt ggtgttctcc cgatttattg accgtctaaa tgagagtttt tgatatataa
      181 ctacagacag ctactataac tttcatctat ttattcacag cgcgaatgaa aaataaaaca
      241 ctctgcttta cagtgtttac tttataatta acagtcttat ctcactgttc accctgtaac
      301 gttcagagag cgtcttcaga tgtctattta taaaattcct cttccgctca atattctgga
      361 agctgcacga gagcgtatta cctggacact caacacccta ccccgcgttt gtgtctcatt
      421 ttctggcggc aaagactctg gtttaatgct gcatctgaca gccgaacttg cccgacaaat
      481 gggcaaaaaa atctgcgttt tgtttatcga ctgggaggcg caattctctt gcactattaa
      541 ctatgttcag tccctgcgcg agttgtacac cgatgtcatc gaagagtttt actgggttgc
      601 gctcccgctt acgacgcaaa attccctttc acaataccaa cccgaatggc agtgctggga
      661 acctgatgtc gaatgggtgc gtcagccccc gcaagatgcg ataaccgatc ctgacttttt
      721 ctgcttttac cagccaggca tgaccttcga acaatttgta cgtgagtttg ccgaatggtt
      781 ttcacaaaaa cgtccggcgg cgatgatgat cggcatccgt gcggatgagt cctacaaccg
      841 ttttgtcgcc atcgccagtt taaataaaca acgttttgcc gacgataaac catggactac
      901 ggccgcacca ggcggtcata gctggtacat ttaccccatt tacgactgga aagtggctga
      961 tatctggacc tggtatgcaa atcatcaaag tctctgcaac ccactgtata acctaatgta
     1021 tcaggcaggc gttcctctgc gtcatatgcg aatttgcgaa ccttttggcc cggagcaacg
     1081 acaaggatta tggctctatc acgttatcga accggatcgc tgggctgcta tgtgcgcacg
     1141 agtcagcggg gtaaaaagtg gcggcattta cgccggacat gacaatcatt tctatgggca
     1201 tcggaaaatc ctcaagccag aacatttaga ctggcaagaa tatgcattat tgctgctcaa
     1261 tagcatgccg gaagatgatc gcatcaaagc gattaatgaa atccgcatgg cgatccatca
     1321 ggttagcccc tttcgtgaag agccagtgga ttgcgttctg tgggttaaaa acagccaact
     1381 tatgcctaat gattacaacc caaacaacgt ggcaccaccc gaaaaaaaac tgctgcaaaa
     1441 atccattgaa atagatggtt ttacccaacc cattgtggtc acacatacgg ataaaaatgc
     1501 catggaaatc gtggatggtt ttcaccgcca tgaaattggt aaaggctcat catcattaaa
     1561 attacgctta aaaggttatt tgccggtgac ctgtctggaa ggtacgcgta atcaacgcat
     1621 cgccgcgact attcgtcata atcgcgctcg tggacgccat caaatcaccg ccatgtcgga
     1681 aatcgtccgt gaacttagcc agttgggatg ggacgataat aaaattggca aagagctggg
     1741 aatggacagc gacgaagtgc tgcgcctgaa gcaaattaac ggcctgcaag agttgtttgc
     1801 cgaccgtcaa tattcccgcg cctggacggt taaatagcta aagctggcgc aggcgttcag
     1861 ctgctgccag caacgtcgat tccttcttgg caaaacagag acgaatcagt ttatgtggga
     1921 agggatcggc gcaaaacacc gacagcggaa tcgccgccac gccgtgctcc cgcgtcagcc
     1981 actggcaaaa ctcaacatca tccagggtag aaacctcgct gtaatccacc agcaaaaagt
     2041 atgtaccttc acacggtaaa atctccagcc ggctttcatt taaagcattc accagaatat
     2101 cgcgcttctg gcgataaaag tccggtaacg caagataatg ctcaggttct gcacgtagca
     2161 tatcagcaag cgccagctgt gccggggtat tcaccgaaaa ggtcagatac tgatgtacct
     2221 tgcgaatttc ggcgctgatg ggcgctggcg caacacaata acccactttc cagccggtca
     2281 tatgatagat cttgccaaat gaagaaaccg ccactgcccg ctcacgcagc tgcggatgcg
     2341 ccagcacact ggcatggccc tgttgtgaaa agttgatgtg ctcgtagact tcatcgctaa
     2401 tgacaaaaat ctcgtgcccg gcgatcgcct gccacaaagc ggcgaaatca gcctgctgcc
     2461 agacagttgc actggggtta tgcggagtgt tgaggatcac cagtctggtg cgctcgctta
     2521 ataatgcggc aaattcctgc cagtcaaggt ggttgcagtg ccatacgctt cactattccc
     2581 ccagaaagcg cgatggcggg ggcgtaactg tcatagctgg gatcaaaaca aatcacttca
     2641 tcgccattgc gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt
     2701 acggtgatat cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca
     2761 atcgcctcgc gcaaggcctg cacgccggtc atgggcgcgt attggtttgc cccctgggca
     2821 acgtggtacg ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc
     2881 gacaggttaa tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg
     2941 ccaagttgtg gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct
     3001 gacgaagttg cgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt
     3061 tagatgtcta aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg
     3121 tcgtcggccc ggctaactac ttttcacatc caagaagttt caatcacctg aacgattttt
     3181 tcactgatga acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg
     3241 cgcaaaccaa acttccgcca gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg
     3301 gtcattgcag cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg
     3361 tggtgattgg cgtcggcggc ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc
     3421 tcggtctgcc atttgttgcc gttccgacga tcgccgctac ctgcgccgcc tggacaccgc
     3481 tctccgtctg gtataacgat gccggacagg cgctgcatta tgagattttc gacgacgcca
     3541 attttatggt gctggtggaa ccggagatta tcctcaatgc accgcaacaa tatctgctgg
     3601 cggggatcgg tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag
     3661 aaacgttgcc gctaaccgtg cggctgggga tcaataatgc gcaggccatt cgcgacgtct
     3721 tgttaaacag tagcgaacag gcacttgccg atcagcaaaa tcagcagtta acgcaatcat
     3781 tttgcgatgt ggtggatgct attattgctg gtggtgggat ggtcggtggt ctgggcgatc
     3841 gttttacgcg tgtggcggcg gctcatgccg tgcataacgg tctgactgtc ctgccgcaaa
     3901 ccgagaagtt tcttcacggc accaaagtcg cttacggaat tctggtgcaa agcgccttgc
     3961 tgggtcagga tgatgtgctg gcgcaattaa ccggggcgta tcagcgtttt catctgccga
     4021 ctacgctggc ggagctggaa gtggatatca ataatcaggc ggagatcgac aaagtgattg
     4081 cccacacctt gcgcccggtg gagtccattc attacctgcc agtcacgctg acaccagata
     4141 cgttgcgtgc agcgttcgaa aaagtggaat cgtttaaagc ctgacgtgca aatttagcag
     4201 cagcgcgcgc cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcctcg
     4261 taggtcatcg gcgtttgatc gggatggtta acccgcatat gttcgacata gttgtcgtaa
     4321 tcaggcatac caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt
     4381 gaatcaaaca tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga
     4441 acgctttatc cggcctacaa atgctatcaa cgcactcggt cagtcccctc gccccatcgg
     4501 ggagagggtt agggtgaggg ggaaccctcg gcactatgcc aacattcgcc aacatccccc
     4561 ctcactctga ctttagtgcg cgcctttcgc ctgcgctacg atctcctcga cattttccgg
     4621 catcggctca tacggcgttt ctttcgccgt tggcttcgga tctttcaatg ccgcaagtgc
     4681 cgtcttaatc gagaacagtg ccagaaccac cacgaccacc ataaagaaga tggttaaccc
     4741 ggcatccaga cggttgttga acaccagttg cgccagctgt gactcagtat actgcgacgg
     4801 aatattgccg ctgtcgatca ttgcctggaa cttgttagca atggccagga agccgacttt
     4861 cgcatccggg ctaaacgctt tctgccagcc agcggtcagg gtacaaatca gcagccaggc
     4921 cgttggtacc agcgccaccc aggcgtaacg ttgacgtttc atcttgaaca acaccacggc
     4981 acagagcatc agcgccatcc ctgccagcat ctggttggca ataccaaaca gcggccacag
     5041 agtgttaata ccgcccagcg gatcgaccac gccctgatgg aggaagtagc cccacgccag
     5101 tacgcacagc gccgttgcca gcaggttagc aggcagtgaa tcggtccgct tcaggccagg
     5161 agacaccacg cccagcagat cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac
     5221 cgccgtcaga ataaacagag cttcaaatag aatggcgaag tgataccaga acgccacatc
     5281 catcatacca cccagcgcac cgtgcaggat gtaagccatc cccaccgcca acgtcggcgc
     5341 accgcccgca cgggaaatga tcgactgttc acccacttcg ctggcaatct ggttgagcgt
     5401 atctggtgta atgctaaagc cccagctact caccacctgc gcggcagaag cgaccacatc
     5461 cgccgtccct gctggagcca gcaccgccat tgggctgttc atggcaaagt aaacgcccgg
     5521 atcgatgata caggcggaaa ccagcgccat aatcgccacg aaggattcca ttaacattcc
     5581 accgtagccg ataaaacaag cctgcccttc gttcgccagc atcttcggcg tggtgccgga
     5641 agagatcagc gcatggaagc cagacaccgc gccacaggcg atggtgataa acaggaacgg
     5701 gaacaggtta ccggtccata ccgggccagt gccatcgaca aatttggtca gcgcaggcat
     5761 ggtcagcgtc gggcgcataa tcaaaatgcc taccgccaga ccgacgatcg tcccgatttt
     5821 caggaaggta gagaggtagt cacgcggggc cagcagcaac cacaccggca gcaccgccgc
     5881 cacaaaaccg taacccacca gcatccaggt cagctgcacg ccggtaaagt caaagtacgg
     5941 tgcccaggtc ggactttctg ccacccagcc gccagagata atggcgaaaa tcaggaatac
     6001 cagaccgatg accgacactt caccaatacg ccccggacgc agatagcgca ggtagatccc
     6061 cataaacagc gccagcggaa tggtgaacgc aacggtatac gttccccacg ggctatgagt
     6121 cagggctttc accacgatca tcgccagtac tgcaaggata atgaccatga tcataaagca
     6181 ggccaccagc gctatcaccc cggcggttgg ccccatctct tctttgacca gctcacccag
     6241 cgatcgaccg tcacggcgcg tagaaacaaa cagcaccatg aaatcctgca ccgcaccggc
     6301 gagaaccacc ccggcgagta gccagatcat ccccggcagg tagcccattt gcgctgccag
     6361 caccggccca accaacggac ctgctccggc gatggccgca aaatggtgac cgaacagcac
     6421 tttcttgtcc gtcggcacat agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg
     6481 cttcgggtca accgccagca catttttggc gatatacaac ccataaaaac ggtaagcgat
     6541 cagatagata cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt
     6601 taaagcaatg tatcccagag caaatgctcc cattacagag agcactgtcc agacgaggta
     6661 tttccctgat ttgttcatag ttgttatccg tgtgcgtgtc catagagatg ttacattttg
     6721 tttctataac atctttgtac gatttaacaa cacggtaact atgtaaatcg gagttgaaac
     6781 caatatttaa cttaacaatg ttaactcgat cactccgtta accgagtttt cctgcaatct
     6841 catttaattc tgtcgggctc atcccaaaac tgccgtaccc agccgacaag tgcagcaacg
     6901 ccgcccctgt tcatcgaaaa cgacgatttc ccagctctga ttttgccgcc caagatgcag
     6961 cggctggcag acgccgcgta ccttgccttc agacaccggg cgatgatggg tggcgttaag
     7021 ttccgtgccc accacacact gcccgtcacg ggtcatcata aatccggcca tcgatcccag
     7081 cgtttcagcc agcgccgccg acgcgccgcc atgcagcagg ccaaacggct gatgagtacg
     7141 ggtatcaacc ggcatttcgg cttccagcac atcatctccc agacgggtat acacaattcc
     7201 cagatgcgcc accattgtgt tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg
     7261 tttccagatc atgcttatgc ccccagcgtt gagccgccat cgaccacaat atcctgtagg
     7321 gtgatatggc tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga
     7381 cgggcgattt tccccagcgg aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc
     7441 tgttcttcgg cgtcatcgct cacccacagc gtgcgttgca tatcggtgtc ggtggagcca
     7501 ggcgaaacca cattacagcg cacgccgcta cccgccagtt ccagcccgac gctcaacgcc
     7561 aggcttttca gcgccgcttt cgatgcgcca taagcactca taccaatacg cggcgtgtgt
     7621 gcggcgtcgg acgccacagt gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg
     7681 gtttgctgga acaggttaaa cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc
     7741 tctttactga gctgatcggt cgcgcccatg cgcaaaattc ccgccgcatt gatcagcacg
     7801 tccagtcgct ccgtttcagc taacagtcgc tgacatacct gcgccacctg cccggcgtcg
     7861 gcaacatcca ttacttcggt cgcaaaggga tattgctcct gagtgaacgc ttgatcaaaa
     7921 cctgtaactt tcgctcccgc ctcaacaaac gccagcgccg tggcgtagcc gataccttta
     7981 cctgcgccgg ttacccagac atttttaccg ctgaaatcca ttatttaacc tcgcgggaga
     8041 gtagcttcca ccaggcgtcg atggtcgggt ttttcgccag catgacaaag tcgatatcac
     8101 catgcacttt gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc agaccgtagt
     8161 cgatcaggtt gtcgtcatcg aacggttcat cggactcgtc cagcaacggc aggataacct
     8221 cacgcagcgc cgctttgctg gcggggacag gtgctggcag taattcttca gtcatcacca
     8281 cccggccaga ccgcccggcc acatatttca gcgacatcaa atgctcgtca cggctgaaat
     8341 cggccagcgc atccgccacc ataaacggtt taatatcgcg cataaatgcg tcggttgcgg
     8401 tggtcataca gccaatgtgg gcatataccc cggtaataat cagctggtta cgtccactct
     8461 ctttgagcat ttgctccagc ggagaacgat gaaacgcgct gtagcgccac ttcaccagca
     8521 ccgtgtcgtc ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc ggcgaacggg
     8581 tcaggcccgg cccccacata tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg
     8641 gctgggcggt gtaataaact gggatattgt gctgtttgca gtagtcgcgc agcgcagcaa
     8701 tattcgcgat cacctgctcc atcatcgggc agttctcgcc ccagaagctg acaaaatagt
     8761 cctgcatatc atggattaac aacgcggcac gttgcagttc aaaggcccag tcaactttat
     8821 tctgcggaat atcgtgagac tccggcagtg cgtaagcctg taattttgga atagccatcg
     8881 tgttctctcc ttcaggctga tgcgcgtgac gccagccact gacttaattg ttttttatcg
     8941 actttcccga ccgccgtcag cggaagtgaa tctacacact ccacgcgatc cggtaattta
     9001 aattcggcaa taccctgttc acgcaggaaa cgacgcacct gcaccgcgcg cagcggctct
     9061 tttaccacca gataagcaca gcttttttcg cccatcagct catcttccat gctcaccagt
     9121 gcggcataga tcaccgccgg atggcgcagc agcaggtttt cgatctcttc ggcagcgatc
     9181 ttctcgccgc cacggttgat ctgatctttc tcgcgcccct gcacggtgat gtaaccctct
     9241 ggatcaatag agatcagatc gccggaacag taaaaaccgt tggcatcaaa ggcgctggca
     9301 ttgtgctgcg gacttttgta ataaccgcgg aaggtgtacg gcccgcgcgt catcaggcgt
     9361 ccgacttccc cttgcggcag tggatttcct tcggcatcgg caacccacac ttcatcgtcc
     9421 ggacacattg ggtaaccctg ggtatggata attttctccg cgctatcatc aagacgggtg
     9481 tagttcacca gcccttccgc catgccaaac acctgctgca actggcagcc aatctcagcg
     9541 ggaatacgcg ccgcaagggt ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc
     9601 gaggcaagct gcgcccggct ttcgccttca gccagcgcct gcaaccacag gctgactgcc
     9661 ggcggcacca gcgcggtgac gttcacctga tgtttttcaa tcaatgggaa gcaaagcgta
     9721 gcgctgggat cggccgccag aacaaccgtt cctccggcaa gaaagacgcc cagcgatcct
     9781 ggcgaactca tggcgtagtt atgagccgcc gggatcgcgc acaggtagcg cgtctgttgt
     9841 gtgaactgac aaatctcgac gctacgacgc acgctgtagt agtagtcgtt atgagtgcgc
     9901 gggatcagct tcggtgtacc ggtggtgccg ccggaaagct ggaaataggc cacttcatca
     9961 gcaggtgatg ccgtggcagt aaaaccgtct gccggatggt taatcgcatc ctgcaagtta
    10021 tgctcaccgc tgtcgttgtg cagttgcacc acgcgaatgg aggaatgttc tgtgacgaaa
    10081 gtattgagga aatcatcccc gctaaacagc gcatgttggc gatcggcaat cagcaatgcg
    10141 ggttcaatct ggctggcata ggcgttcagt tcactacgct gatggctgaa caacgccagc
    10201 accggcgcaa cgcccagttt cagcagcgcg aaaaaggtaa tatacaattc agcgacgtta
    10261 cccagttgta ccagcgcggt ttcaccaggt ttaatgccct gacggcgtaa agtacacgcg
    10321 aggttatccg ccgcctgatt cagctcccgg taactcaact gtagctcgcc gtcgataacc
    10381 gcgatgctgt cactcgcagc gtggcgagtc agaatgtcgg tcagcggcaa atcctgccag
    10441 tagccttttt cccgatagcg acgggcaaac tcttccggcc agcgggtgaa tggaatgctc
    10501 atcctcgctc cttaatgcaa tccaaaaaca ttcaacatgg tagaaagttt gacgcctgtt
    10561 tcgcgccact cacccaacgg tgacgacgca agcacaatcc ccgctccggc aaacagacgc
    10621 acctgatttt cccgcagctt cgcgcagcgg atggtcacca cccattcgcc gttaccttcg
    10681 ctgtcacacc aacccacaat gccgccaaac agttcgcggt cgaacggctc cagttcagca
    10741 ataacctgag tcgcggcctg atgcgggaaa ccgctcagcg cgggggtcgg atgcagcaga
    10801 caggccagag tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga
    10861 tgccacagcg tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa ctcactactg
    10921 cgttcgcgca gtacctcttt catcgcctga gtcaccagtt catgttcatg gcgatctttt
    10981 tctgacgcca gcagacgatt acccgcttcg cgatcgagca cttcatccgg ctgacgacgc
    11041 gcggaaccgg ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc
    11101 gggctggccc ccagcaggac gccaccatca gccagcggga catggaagtt gtaactaacc
    11161 gggttttgcg caatcaaccg ttccagcaat acgccactat caatggcggc gtcagtggtg
    11221 atatcaatca accgtgacaa caccactttg tcgacctgcg gcgtggcggt aagtgcggca
    11281 gcgcgggcaa ccatctgttc aaacgtggtt tgttccggaa ttgcctggcg ttccaccaca
    11341 ttcagcgact ggctgcgggt gaaacggcgg gcggaagctt gtttttcctg acgggagaac
    11401 gactgccagg attcaggaat atacaacgac gaaggctgac gtggatcgaa gggaatcgcc
    11461 ccgaccatca ccggattttt gatgccctgc actttggcat cggcaaacag cgcggcgagt
    11521 ttttgctgga agggactgtc gggcgaatcc ccgttcacag ccggttcatc gaagcgggcg
    11581 aaacatcctg acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg attgggcgca
    11641 agtgttgcca tggtctgctg tacttcctca gccagtgacg tatccatatc atcctccaca
    11701 aaatgataaa ggctttaata atgattatca tttatatttt cggtcgctaa cctaaaagca
    11761 aacgcgcact atgtcaactc ttgaggtaac gcaatctgca actcggggtt gcatctggtt
    11821 gtggttacaa tgaaaatgag aagcattatt gatggattcg cataagcgca atgtgatgtc
    11881 ctgcgccgtt cttccccctc tcccttccag ggagagggct ggggtgaggg tcaatattcg
    11941 caccagtgct ggctgctccc atcaccctaa ccctctcccc aaaggggcga gggtacggat
    12001 agtgcgcttt gtcgaatttg tcattacgcc cttaacctta ttaataacag gaagctgatt
    12061 tgtgagactc gccccgctct accgcaacgc ccttctatta acaggacttt tgctttccgg
    12121 aatagccgca gttcaggccg ccgactggcc gcgtcagatt actgacagcc ggggcactca
    12181 taccctggaa agccagccgc agcgtattgt ttccaccagc gtcaccctga ccggctcact
    12241 gctggcgatt gatgctccgg tgattgccag cggcgcgacc acgccaaata accgcgtcgc
    12301 ggatgaccag ggctttttac gccagtggag caaggtggcg aaagaacgca aactacaacg
    12361 gctctatatc ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg atctgatttt
    12421 aattagcgca accggcgggg attcggcgct ggcgctgtat gatcagcttt ccaccatcgc
    12481 cccgacatta atcatcaatt acgacgacaa aagctggcag tcgctgttaa cgcagcttgg
    12541 cgaaattacc gggcatgaga aacaagcggc agagcggatt gcacagtttg ataagcaact
    12601 ggcagcggcg aaagagcaaa tcaaattacc gccgcagccg gtcactgcta ttgtctatac
    12661 cgccgctgca cacagtgcca atctctggac gccggaatca gcacaagggc agatgctgga
    12721 acaactcggc tttacgctgg cgaaattgcc cacagggtta aacgccagcc aaagccaggg
    12781 caaacgccat gacatcattc agcttagtgg agaaaatctg gctgcagggt taaatggcga
    12841 gtcactattc ctgttcgccg gtgatcagaa agacgccgat gcgatttatg ccaatccact
    12901 gctcgcgcac ctgcccgcag tacaaaacaa gcaggtttat gcgctgggaa ccgagacgtt
    12961 ccgcctggat tactacagcg ccatgcaagt gctggaacga cttaaggcgc tgttttaagc
    13021 attaactgtc ggacgctgtc acctgcggcg gcgtctggcg aaaacgtcgc aactccacca
    13081 gcaccagcaa taacaacacg ccgataatca acaaaccaaa accgctcgcg cttgcggaag
    13141 caaccggtgt catcatcgct cccagaccgc ctaacagcgc cgcgcctatg gcatcgcccg
    13201 tcacgttttg tgccgtccac aaaccgttga ttcgccctaa cattgcttcc ggcgtttgcg
    13261 tttgcagcat tgtatattgc agcaacgaac tgaccgcact cagccagccg aacagcgcca
    13321 gacaaaccac gcctaaaatc cacatcggca tcaggccaaa taaaccaatg gcgaggaacg
    13381 atcccaacgt ggagagcaac atcaacaatc ctggtcgcgc gctatgcgcc agtttcccac
    13441 tggttaatgc gccaatagcc gcaccgagcg ggatcgccgc atagagaaaa ccaatctctg
    13501 ccgccgacat ctgccagttg tcagccagcg ccggatacag tacccgcacc gcgctcgcca
    13561 tcgtcaataa accacccagc aacgcaatcc cgcccaccag cgggctggcg agcagaaaac
    13621 gaaatcctgc cagtaatgat ttcaacggat gctcgcgcgg ctgcggtggc ggtggcaacg
    13681 ccggaaggct taacaacggt agcaaggtaa taaacgtgcc cgccgccgcc agcccgtagt
    13741 tccaggctac gccaccggtc gccagcaata aaccgccaat catgggcgaa atcaccgacc
    13801 ccagacgcac ggtcaacatg gtgatcgccc cggcctgcat taagttttca cgccctacca
    13861 gtgctggtgt cgccgccaat agcgccgtaa cgccaagcga tgcgaaaaaa ccatcccata
    13921 aaccaagtaa atagattgcc agcaatgacg gctccggcag cagtgcatta aggcacagtc
    13981 caatgaagcc aatgccacag gtgccgcgcg ccagcaaaat cacttttttg cgctcatagc
    14041 gatccgccag cacaccgcca accatcaggc caacaaacat cgcgccgccg gtcagcgtca
    14101 ccgaaagccc caccagccag gtagaatgcg tcatcatctg gatctgcacc ggcaccgcga
    14161 cgccgagcaa acccagagac acaattgaga taaaacgagc gaggaatact gcgcgaaacg
    14221 ccgggtgcgt tttcaacagg ctgaggttaa gcagccagga ttgtttattc attacaatgc
    14281 cttgccatca aatattttaa atacctattc ttaggctgca catgctaaca tatccaaata
    14341 agatcgataa cgataattaa tttcattatc atggaagttc gtatgtctgg ttctgttgcc
    14401 gtgacacgcg ccattgccgt gcccggattg ctgttattac tgattatcgc gacggcatta
    14461 agcctgctca ttggggcaaa atcactcccc gcttccgtag tgctggaggc cttctccggc
    14521 acctgccaga gcgccgactg caccatcgtg ctcgacgcgc gactgccgcg cacccttgcc
    14581 ggtttactgg caggcggcgc gcttggcctt gccggggcat tgatgcaaac cctcacccga
    14641 aatccacttg ccgaccccgg cttgcttggc gtgaacgccg gagccagctt tgccattgtg
    14701 ctgggtgcgg cgctgtttgg ttactcatcc gcgcaggaac aactggcgat ggccttcgcc
    14761 ggggcgctgg tggcctcatt gattgttgcc tttaccggca gtcagggcgg cgggcagtta
    14821 agtccggtgc gtttaaccct ggcgggcgtg gcgctggcgg cagtgctgga agggctgacc
    14881 agcggcatcg ccctgcttaa tcctgacgtc tacgatcaac tgcgtttctg gcaagccggt
    14941 tcgctggata ttcgcaatct acatacctta aaagtggtgc tgatcccggt gctaatcgcc
    15001 ggagcaaccg cgctattact gagccgcgcg ctgaacagtt taagcctcgg cagcgacacc
    15061 gcgacggcgc tgggcagtcg cgtggcgcgc acacagttaa ttggtctgct ggcgattacc
    15121 gtgctttgtg gtagtgcgac agcggtagtt ggcccgattg cctttattgg cctgatgatg
    15181 ccgcatatgg cgcgttggct ggtgggtgcc gatcatcgct ggtcgctgcc cgtaacgctg
    15241 cttgctaccc ctgccctgct gctgtttgcc gatatcatcg ggcgggtgat tgtgcccggt
    15301 gaactgcgcg tttctgtggt cagtgccttt attggtgcac cggtgctgat cttcctcgtg
    15361 cgacgtaaaa cgcgaggtgg tgcatgattt acgtctctcg ccgattactc atcacctgtt
    15421 tgctgcttat ttccgcctgt gtggttgcag gtatctgggg attacgcagc ggtgccgtca
    15481 cgctggaaac ctcgcaggta ttcgccgcgc tgatgggcga tgcgccgcgc agtatgacga
    15541 tggtggtcac cgaatggcgt ttaccacgcg tgctgatggc gctgttgatt ggcgcggcac
    15601 tgggcgtcag cggtgcgatt tttcagtcgc tgatgcgtaa cccgctcggc agccctgacg
    15661 taatgggctt taacaccggg gcgtggagcg gcgtgctggt ggcgatggtg ctgtttggtc
    15721 aggacctgac ggctatcgcg ctggcagcaa tggtgggcgg cattgtcact tctctgttgg
    15781 tctggctgct cgcctggcgt aacggtatcg acacctttcg gttgattatt atcggcatcg
    15841 gcgttcgcgc catgctggtg gcctttaata cttggctgtt gctgaaagcg tctttagaaa
    15901 cggcgctaac agcgggtttg tggaatgccg gatcgctcaa tggcctgacg tgggcaaaaa
    15961 cttcgccttc cgcacccatc attatattga tgctcattgc cgccgccttg ctggtgcggc
    16021 gaatgcgctt gctggagatg ggcgatgata ccgcctgcgc gctgggcgtc agcgtcgagc
    16081 gttcgcgtct gttaatgatg ctggttgcag tggtgcttac cgctgcggca acggcgcttg
    16141 ccgggccgat ttcctttatt gctttagtcg cgccgcacat tgcccgacgc attagcggca
    16201 ccgctcgctg ggggctgacc caggcggcgc tgtgcggagc gctgttactg ctggcggccg
    16261 atctctgcgc ccaacaactg tttatgccgt atcaacttcc ggttggcgtc gttaccgtca
    16321 gcctcggcgg tatttacctt atcgtcttgt taattcagga gtctcgcaaa aaatgaccga
    16381 atcagtagcc cgtttgcgcg gcgaacagtt aaccctggga tatggcaaat ataccgttgc
    16441 ggaaaatctg actgtagaaa ttcctgatgg tcacttcacg gcaattatcg gaccaaatgg
    16501 ctgtggtaaa tccacgttac tgcgtacctt aagccgcctg atgacgcctg ctcacgggca
    16561 tgtctggctg gatggcgagc acattaaaca ttacgccagt aaagaagttg cacgccggat
    16621 tggtctgttg gcgcaaaatg ctaccacgcc gggcgatatc accgtgcagg agctggtggc
    16681 gcgtggacgt tatccgcatc aaccgctgtt tacccactgg cgcaaagagg atgaagaagc
    16741 ggtaacgaaa gcgatgcagg ccacgggaat aactcatctg gcagatcaaa gcgtggatac
    16801 cctttctggc ggacaacgcc agcgagcgtg gatcgcgatg gtgctggccc aggaaacggc
    16861 aattatgctg ctcgacgaac ccacgacctg gctggatatc agtcatcaga ttgatctact
    16921 ggaactgtta agcgaactga accgcgagaa aggctatacc ctggcggcgg tgctgcacga
    16981 tcttaatcag gcctgtcgtt acgccagcca tttgattgca ttgcgggaag ggaaaattgt
    17041 tgctcaggga gcgccgaagg agattgtcac tgctgaactg attgagcgca tttatggtct
    17101 gcgatgcatg atcattgacg atccggtagc cggaacgccg cttgtggtgc cgctcgaacg
    17161 aacggcacct tcaaccgcaa atagttaaac taagtggtct gccatcatgg catcctgttt
    17221 tctggatgcc atcgcatagc gcaataacac gctaccacaa gccaccatcc cgccgatcag
    17281 cgcggaaagg atcacaataa tcgccttacc cggaccgtct tttttcactg gcaatgacgg
    17341 gcttaactga tatttaaacg gcgtaaaatt cacatcgttg acatttgctt ttgttaattg
    17401 ctcgacaaga tactgccgat tacgtaattc accgttcagt tccgcaacgt cagtaaccgc
    17461 tttttcaatt tccagtttgc gttcaatacc gtctgcgccg agagaaatag agaaatcggg
    17521 gtcatcttta acggcctgac cattactgta tacgggcttt ttaattcctg ccgcgttggc
    17581 aatgtcgagt gaataattga ggcgctgaat gtttgcatca agttgatttt tcgttttaat
    17641 gcgatcctga gccagttttt ctttttcaaa ctgggttttg atctccagtt tattacggac
    17701 gttttctatc gactctttca ccaccaacgc agagatataa tcgatatacc cgctcaaaac
    17761 ggtctgcgcc tctttactgg ttggcgcggt aaaacttagc gtccaggagg tatacagtga
    17821 cggttcatct tttttcttac tggcattgtc atcaaccgct ttcatttttt cgctcaatgc
    17881 aacaatagcg cgatgcaaat ccagttcatc gattttcgcc tcttttaatt ggtccatcac
    17941 ataaggtgat gaacgcaggt actcttccag caagctaaca gactgaaact tcttgataaa
    18001 caggttaaat gcaccgccac ggtcgatatt aacatcgaga tcaagaacgc ggagtttggt
    18061 aaaggttttc tccaggtcct gccactgaat ggcttcggcg ggagtaataa cggctgagct
    18121 ggtccatttt tgcggcagga tgaaagagat cagtaaacct gcgcaggcaa atgcaaaaac
    18181 gaccgccatg accgtttttt tggcccgcca taaaacttcg attagattaa gtaaatcaat
    18241 ttcattacta ctgggcgacg ccagaggata ctcggaaaaa tgagcgtcac ttccctgttt
    18301 aatattcagt gatgacatgg gtataacctg aaaatgagtc caataggggg aaatctccac
    18361 ggcgctcact ttaccagttg gtgagaatta tccgaagctg aagtttgtaa attccttcca
    18421 ctgaatgcaa cattcactct atttaagaga aatattcatc aaacctataa atcaaacaaa
    18481 taaaaaaccc ataattattt tctgtaatta tgggtttata gataatatta atttacctgt
    18541 ttagcgttgc gcgaataatc ggcccaattt ttacaaacgc ccctggagag ataatatcca
    18601 catgcgcaca atcctgacga tagatatcca gctcggcaat ccagggcgac caagcgcgtt
    18661 cggggctcat accttcctga agcgtgcgtt cagcaacaaa cagcgtcgct ttaccatcaa
    18721 acggtacgct atgagcagtc gtcagcagac gcacagcatc agcgtagttg ccttcaatgg
    18781 tggtaaacaa ctccgttgaa gtacttccct gctgtgccgc caggaaggcc tcgcgttcgc
    18841 ggttaatctc cgccagcact tccgggtcca gaccattagc ctctttttcc tgccagtttt
    18901 gcgtttctgg cggccaggta tccagcaaac caagaaatgc cacctgttcg ccacgggcac
    18961 gcagtcgcgc cgcaatgccc tgcgccagcg taccgccaag ggaatacccc agcaggtaat
    19021 aagggccgtg cggttgttgt tcaagtaacg ttgccagatg cgcttcacag acttcatcca
    19081 gatttgccgc ggtatgcatg gggccattgg ggcgcggcga ctgaatgccg ataatcgacc
    19141 attgtggatc gatataacgc gagagcacgc taaactgcca ggcaaaaccg gatgccggat
    19201 ggaaacaaaa cagcgtcggg ccattacctt cacgcaacgg cagaatggtt tcgaatccca
    19261 tacgccgggt gctgtcttct tcagcatcaa taatcgttgc cagtttagcg acggttgacg
    19321 cgaccatcac ttgccccggc gtcacctggc gggcaacctg ccgacttaac tgcgctgcca
    19381 gtttcattgc cagtagcgaa tgaccaccaa gcgcaaagaa atcagcatcg gcatcctgca
    19441 cgtcacaacc cagcaacgac gcgaatgccg cggcaataat cgtttcactg cccgcttttg
    19501 gcgcacgccc cggcgcttgt gccttcagtt caggcaacgg taaggctttg cgatccagct
    19561 tgccgttggc gctaagaggt aacagtggaa gttgcagcag aaccacgggc accatatgtg
    19621 gtggcaatgt ttcgcgaagc tgcgcctgta atgcgctggt atccaacggc agacctgatt
    19681 gcgacaccag atagcccacc aactgacgcg catcaccacc ggtggctgcc gcctggttaa
    19741 tcacacaggc gtgggtaacg gcttgttcga catccggcag cgcctgcatc acgcgatcga
    19801 tttcgcccag ttcaatacgc tgcccgcgaa tttttagctg atcgtcactg cgcccgaggt
    19861 actccaccgc gccgttatcc agccagcggg caacgtctcc ggtacggtac atccgttcac
    19921 cagggacaaa aggatcggca ataaagcggc tggcggtcag atcgggtcgt ccaagatacc
    19981 cctgcgccag ttgaatgccg gtgagataga gatcacccgc cacacccggc ggcaccggat
    20041 gcatcatcgc atcaagaata cgcagacccg tattccatac cggataacca atcggcacac
    20101 tgctgccgcg cacctgtgcc agttcctcgc caaaagccgg ataccagctg acatctaccg
    20161 ccgcttccgt cgggccatat agattatgca acggcacgct ggttaactgt tgccattcgc
    20221 ggcataaatc agccggtaag gcctcaccac tacagaaaac ctgtttcaac gtcgcgcaac
    20281 tctggcgagc ggtttgcggc gtcagcgatg caacaaatgc tgccagcatc gacggcacaa
    20341 agtgcgtggt cgttacgcca tattcggcaa agaattgctg catagcgagc gggtcgcggt
    20401 gcgcttccgg ttcagccatc accagtttag ccccggcaat aaacggccag aaaaactccc
    20461 acaccgagac atcaaaactg cacggcgttt tttgggcaac gacatcttcg cctgtaagca
    20521 gataatgatt ttgcatccac aacaggcggt taacgatagc cgtctgcccg accattaccc
    20581 ctttcggcct gccggtggag ccagaggtaa agatgatata agccgtgtga tgcggttgtg
    20641 aaagttgcag cggcgcactg ccctgcggtt taagcggggc gttatagcaa aggttggtta
    20701 aatctggaac atcggcaaag cgcggcagtt gatcgtcggt ggtgattaac agcgacggac
    20761 gcgcatcttc cagcatcatt ttcaggcgat cgtccggata gccggtatcc agcggtagcc
    20821 aggccgcgcc agcttcaact atcgcatgta gcgccagggt caaaaagacc gagcgcggta
    20881 gcgccaccgc cacgctgtcg cccggtttaa cgccacgctc acgcagcaga ttcgccagcg
    20941 ccaccacctg ctcgcgcatt tcccgatagc tgaactggta acgcgcatct gccagcgccg
    21001 gagcatccgg tgtttttgcc gcttgttctg ccaccagcgc gctcagcgtg gtttctggaa
    21061 tctcaacctg agtggcgttg atctgcgcca gctgcgcata ctcacctggc agcataatat
    21121 cgacatcgcc gcacaacagc gccggatccg cggcgaactg ggcaatcagc attttcaggc
    21181 gttcagcatg ctggattaac gttggctcat cgtaacgctg tttattggcg aggatctcaa
    21241 tactcaaatc accgtgttca tccgggaaca gggccagttc aaggtcatta accggaccgg
    21301 ttgccaaggt atgggtttgc gcctgaacac caggaatatc cagttggtaa tcaaatacct
    21361 tgatattgag taccggacca aacagcggtt catcacctgc cgctcgcccg ctgtcacgga
    21421 caatttgttc ggcatcgtaa cgttgatgac gacgcatttt tttcagttgt gctgccagtc
    21481 gggttgccag ttccggcagc gtttcttgcg ccgcaatgtg aatacccaac ggcaaaacgt
    21541 tgagcacggg tccggtagcc gtcagcgccg ccgagcccag tcgacgcata aagataaatc
    21601 cagcggcgta gtccatgcga ttgcacaatc gccccagcca caaagccgtc agcgcaaggg
    21661 ctaaatcggt acgctgcaca cctgaaagtt gcgtagccaa ctggcggaat tccccgtcgg
    21721 taaattccat tttcagacgt aaaatatctg ccgaagcgct gcgccccggt aaaggtgccg
    21781 gagaaagtga cgcgggcggc ggcagttgac gacgctgttc tgcccagaat gccgcatcac
    21841 gctgccaggc ttcgctttcg cggtattgct ggtactcttc cactacatca gcgaaaggcg
    21901 taaacggcga agcaggcgtt ggttcaccac gcagccatgt gcagtaaata ttggcgatct
    21961 ggcgggtaat ggccgggaaa ctgaagccat cgaccagcaa atggtgataa cgctgatacc
    22021 agtaccagcg gttatccgcc acctgtatca gctgatgaaa gaccagtggt ttaccgctat
    22081 cgacgcgcag atcttgttgc aaatccgcct gcattaatgc ctgcgcagta ccgtgcggat
    22141 caatgttggt tcgcaggtcg ataatttctg gcagttcgaa cgtcagcgca tcatcgaccc
    22201 actgccagac ttcgccgtta tcttccgtaa aacgcatccg cagcgtatct gcttgcgcta
    22261 gtccgtcaac caccgcgcgg gccagtaatg gcgcatcaac ctctccggtt aactcaacgt
    22321 aatgcgctac gctccaggcg gagggtaaat ccgacagttt ttctgccatc cagatgccgg
    22381 gctgtgcggc gaccaaaggt aaatgctggc tcattgcgcc tcctgcaact gggtgaaatt
    22441 cgccggtgtc agagtacgcc agtgggcttc aagccactgc tggcaggact cttgtgactg
    22501 cggctgacac acaatgtccc agcctgccgg taaggcgcat tgttgcggcc acagactgaa
    22561 ttgcccctgc gcattgcgca aaatgtaaaa cgctccctgt ggatcatcga agggattact
    22621 gaatgccata ttcaactcct gtcatggaaa agtggttgcc agaggtcgat tagcccctgc
    22681 atcaagccac cgcgccaaca aagcgcatca tgtccgccgt caacctgacg ccagaaaatg
    22741 gattctttta tggggtgtaa ttgcgcatac agcgcctgat tggcccgcat aatcatcggc
    22801 tcgcgaatac ccgcttccag cacaatgcgc agaccttcgg cgctaacttc accagcttta
    22861 agcttttcaa gtaacacgcc ctcttgctgc ccgccccgat gcggccacca gtacgatcct
    22921 gactggctta atacacaacc aaaacgttca ggccagtgca gtccggcata cagcgcggaa
    22981 agtccaccaa aactctgccc ggcaaccacg gtgcgatcgg cgcgatcgct aaaaggggca
    23041 atagctttca ccaggggtaa taactcttgc tgtactgcga gccagaaatc cgcattacac
    23101 ggcagttcgt gagcgcggtg cgtggtgtcg atagcgtcga tcaacacata cacggcggga
    23161 ggaagttgct gacgatgggt cagcgaagtc agcactggcc agacgggcat actttgcgcc
    23221 caaaattcgc catcgagcaa aactgccagc gggcgttctt ctgctgttgc atcgccggtg
    23281 gtaaaaatcc atacacgacg tgaatttttc aaccgttcac ttttccagat aatttctttg
    23341 gctggtattt ctggcgcttg cggacaatcc catcccggtt gcagaggcgc ttgcggcatt
    23401 tcgagtgcag aaacagcgtg ccctcgcccg cctttccagc tttgtggatt cagcggatcg
    23461 gctattgcct ggggtaatag ttttcgccag ccttcacgta attcgaggcg atcgggggat
    23521 ggtgcagaaa aaatgtcatc gcgttcggtg ggaataaagc agtagctgcc gcgccagttg
    23581 gcattgagtt gtgtcgtcca ctgccagaca tcagtacctg caatgcgctg catcgactgg
    23641 ggctggctgt tctgatgatg atcggtcaca ccagtgatgt agacccatac gcgctttatc
    23701 ggcgagtatt cttcagaacc ttggggctca cgccaccaga aagtgacctc aaacatttcg
    23761 tcattcagac gctgccattc cgggccatgt ttcgactgcc accagctctc acttcctact
    23821 tttaacgccg tcacaccata accccatgtt tactgtgcaa tttttcattg attgcagaaa
    23881 tatattgata atattattga taactatttg catttgcaat agcgtaatgg cgcgccgtgg
    23941 gaagcgcgga cattaattaa ccaactgcac tgcgtgtctt tcaggatcaa aggttttcgc
    24001 ggtagcggga tgcgtcgtgt tgaggacgac catgcccgac agttgcaatt cgtggcaaaa
    24061 atgcaggaat aaaacaatga acaagaagat tcattccctg gccttgttgg tcaatctggg
    24121 gatttatggg gtagcgcagg cacaagagcc gaccgatact cctgtttcac atgacgatac
    24181 cattgtcgtt accgccgccg agcagaactt gcaggcacct ggcgtttcga ccatcaccgc
    24241 agatgaaatc cgcaaaaacc cggttgcccg cgatgtgtcg gagatcatcc gtaccatgcc
    24301 aggcgttaac ctgaccggta actccaccag tggtcagcgt gggaataacc gacagattga
    24361 tattcgcggt atgggtccgg aaaacacgct gattttaatt gacggcaaac cggtcagcag
    24421 ccgtaactcg gtgcgtcagg gctggcgtgg cgagcgcgat acccgtggtg atacttcctg
    24481 ggtgccgcct gaaatgattg aacgtattga agttctgcgt ggtccggctg cagcgcgtta
    24541 tggcaacggc gcggcgggtg gcgtagttaa catcattacc aaaaaaggca gcggtgagtg
    24601 gcacggttcc tgggacgctt atttcaatgc gccagaacat aaagaggaag gtgccactaa
    24661 acgcaccaac ttcagtctga ccggcccgct gggcgacgaa ttcagcttcc gcttgtatgg
    24721 caacctcgac aaaacccagg ctgacgcgtg ggatatcaac cagggccatc agtccgcgcg
    24781 tgccggaacg tatgccacga cgttaccagc cgggcgcgaa ggggtgatca acaaagatat
    24841 taatggcgtg gtgcgctggg acttcgcgcc tctgcaatca ctcgaactgg aagcgggtta
    24901 cagccgccag ggtaacctgt atgcgggcga tacccagaac accaactctg acgcttacac
    24961 tcgctcgaaa tatggcgatg aaaccaaccg cctgtatcgc cagaactact cgctgacctg
    25021 gaacggtggc tgggataacg gagtgaccac cagcaactgg gtgcagtatg aacacacccg
    25081 taactcgcgt attccggaag gtctggcggg cggtaccgaa gggaaattta acgaaaaagc
    25141 gacacaggat ttcgtcgata tcgatcttga tgacgtgatg ctgcacagcg aagttaacct
    25201 gccgattgat ttcctcgtta accaaacgct gacgctgggt acggagtgga atcagcaacg
    25261 gatgaaggac ttgagttcca atacccaggc gctgaccggg gcgaacaccg gcggcgctat
    25321 tgatggtgtg agtgccaccg accgtagccc gtattcaaaa gcagaaattt tctcgctatt
    25381 tgccgaaaac aacatggagc tgactgacag caccatcgta acgccggggc tgcgtttcga
    25441 tcatcacagt attgtcggca ataactggag cccggcgctg aacatatcgc aaggtttagg
    25501 cgatgacttc acgctgaaaa tgggcattgc ccgcgcctat aaagcgccga gcctgtacca
    25561 gactaacccg aactacattc tctacagtaa aggtcagggc tgctatgcca gcgcgggcgg
    25621 ctgctatctg caaggtaacg atgacctgaa agcagaaacc agcatcaaca aagagattgg
    25681 tctggagttc aaacgcgacg gctggctggc gggcgtcacc tggttccgta acgattatcg
    25741 caataagatt gaagcgggct atgtggctgt agggcaaaac gcagtcggca ccgatctcta
    25801 tcagtgggat aacgtgccga aagcagtggt tgaaggtctg gaaggatcgt taaacgtacc
    25861 agttagcgaa acggtgatgt ggaccaataa catcacttat atgctgaaga gtgaaaacaa
    25921 aaccacgggc gaccgtttgt cgatcatccc ggagtacacg ttgaactcaa cgctgagctg
    25981 gcaggcgcgg gaagatttgt cgatgcaaac gaccttcacc tggtacggta agcagcagcc
    26041 gaaaaaatac aactacaaag gtcagccagc ggttggcccg gaaaccaaag aaatcagtcc
    26101 ttacagcatt gtgggtctga gcgcgacctg ggatgtgacg aagaatgtca gtctgaccgg
    26161 cggcgtggac aacctgttcg acaaacgttt gtggcgtgcg ggtaatgccc agaccacggg
    26221 cgatctggca ggggccaact atatcgcggg tgccggggcg tatacctata acgagccggg
    26281 acgtacgtgg tacatgagcg taaacactca cttctgatgc taacgtcaga ttgttgataa
    26341 agtgcgcgtc gttcatgccg gatgtggcgt gaacgcctta tccggcctac aaaaatcttg
    26401 ccaattcaat atattgcagg accatgtagg cctgataagc gtagcgcatc aggcagttta
    26461 gcgtttgtca ccagtctcta atatggtcga catgaaaact acgcatacct ccctcccctt
    26521 tgccggacat acgctgcatt ttgttgagtt cgatccggcg aatttttgtg agcaggattt
    26581 actctggctg ccgcattacg cgcaactaca acacgctgga cggaagcgca aagccgaaca
    26641 tttagccggg cggatcgctg caatttatgc gctgcgggaa tatggctata aatgtgtgcc
    26701 cgcaatcggc gagctacgcc aaccggtgtg gcctgcggag gtatacggca gtattagcca
    26761 ctgcggggct acggcattgg ccgtggtttc ccgtcaaccg attggcgttg atatcgaaga
    26821 aattttttct gcgcaaacag cgaccgaatt gacagacaac attattacac cagcagaaca
    26881 cgagcgactc gcagactgcg gtttagcctt ttctctggcg ctgacactgg cattttccgc
    26941 caaagagagc gcatttaagg caagtgagat ccaaactgat gcaggttttc tggactatca
    27001 gataattagc tggaataaac agcaggtcat cattcatcgt gagaatgaga tgtttgctgt
    27061 gcactggcag ataaaagaaa aaatagtcat aactctgtgc caacacgatt aattgacaac
    27121 atctggtacg attcgcccgc agccatcact gaccgcgggc gaaagtgtaa agcaggtgcc
    27181 ttaccatcct gacctgacaa ccggatatgc ggggattgct ccccgcacaa cggctacttc
    27241 ttcggttcgt aagcgagaac agccttaaac tcaatattac gttcctttac ggtgaactca
    27301 cacagcgaat ccccgaccag aagcgtaaat cccagcaccg ttaaacacag cactatgact
    27361 gccgcaaggg catatttcgt cagcatgttt atattgcctc catgcgaaga agggactacc
    27421 atccaaattg ttcaggtttg ggtaggtagc cccagggttg atagaaatat cgtcctgggg
    27481 cttcatcttt ccagacctca gagtaaacct gaaaccggaa agcttcaggc acccgcagtt
    27541 atcttaccct tgcttagcaa aatagctgtt tatttataca gtaagtcctg tttcggactt
    27601 ctcactcccc atttgatcaa ctgaagctga acccgttttt ttctgcatgc ctcaatcgtc
    27661 aaaattcttc gccaccagtc agcactgcca taacttgtca cattgcaaaa gtagccaaat
    27721 tgcttctgtc ataatcagtt caccatcacc aaaaaaaaga aacattccag aacttccgca
    27781 tctcgcccag ccgctcccac ttctatactg aatagaaaac gccaacataa gagaaaccta
    27841 tgccattacc cgattttcat gtttctgaac cttttacact cggtattgaa ctggaaatgc
    27901 aggtggttaa tccgccgggc tatgacttaa gccaggactc ttcaatactg attgacgcgg
    27961 ttaaaaataa gatcacggcc ggagaggtaa agcacgatat caccgaaagt atgctggagc
    28021 tggcgacgga tgtttgccgt gatatcaacc aggctgccgg gcaattttca gcgatgcaga
    28081 aagtcgtatt gcaggcagcc gcagaccatc atctggaaat ttgcggcggt ggcacacacc
    28141 cgtttcagaa atggcagcga caggaggtat gcgataacga gcgctatcaa cgaacgctgg
    28201 aaaactttgg ttatctcatt caacaggtga ccgtttttgg tcagcatgtt catgttggct
    28261 gcgccagtgg cgatgacgcc atttatttgc tgcacggcct gtcacggttt gtgccgcact
    28321 tcatcgccct ttccgccgcg tcgccatata tgcaaggaac ggatacgcgt tttgcctcct
    28381 cacgaccgaa tattttttcc gcctttcctg ataatggccc gatgccgtgg gtcagtaact
    28441 ggcaacaatt tgaagccctg tttcgctgtc tgagttacac cacgatgatc gacagcatta
    28501 aagatctgca ctgggatatt cgccccagtc ctcattttgg cacggtggag gttcgggtga
    28561 tggatacccc gttaaccctt agccacgcgg taaatatggc gggattaatt caggctaccg
    28621 cccactggtt actgacagaa cgcccgttca aacatcagga gaaagattac ctgctgtata
    28681 aattcaaccg tttccaggcc tgtcgctatg ggctggaagg cgtcattacc gatccgcaca
    28741 ctggcgatcg tcgaccgcta acggaagaca ccttgcgatt gctggaaaaa atcgcccctt
    28801 ccgcacataa aattggtgca tcgagcgcga ttgaggccct gcatcgccag gtcgtcagcg
    28861 gtctgaatga agcgcagctg atgcgcgatt tcgtcgccga tggtggctcg ctgattgggc
    28921 tggtgaaaaa gcattgtgag atctgggccg gtgactaagt agggaattgc cttacagaag
    28981 aatatccctg acaatggtgt tttaacttac atttaacaat gttatgaaac accctttaga
    29041 aaccttgact accgcagcag gcattttgct gatggctttc ctctcttgcc tgctgctgcc
    29101 cacccccgca ctggggctga cgctggcaca aaaactggtg accatgttcc atctgatgga
    29161 tcttagccag ctttacactt tattgttttg tctgtggttt ttagtgctgg gcgctattga
    29221 gtattttgtt ctgcgcttta tctggcgacg ctggttctcg ctggcggatt aaacgtcgat
    29281 tatcgccgta cttcgcggca tactttgctt attctccttc gcctaaagga acgtttatgg
    29341 ataagcaatc actgcacgaa acggcgaaac gcctggccct tgagttaccc tttgtcgagc
    29401 tttgctggcc ttttggcccg gagttcgatg tttttaaaat tggcggcaaa atttttatgc
    29461 tgtcgtcgga gctgcgcggc gtccccttta tcaatctgaa gtccgatcca caaaaatccc
    29521 tgttaaatca gcagatatac ccgagcatta agccagggta tcacatgaat aaaaagcact
    29581 ggatttcggt gtatcccggc gaggaaatct ccgaagcgtt acttcgcgat ctgatcaacg
    29641 attcgtggaa tctggtggtt gatggtctgg ctaaacgcga tcaaaaaaga gtgcgtccag
    29701 gctaaagcgg aaatctatag cgcatttttc tcgcttacca tttctcgttg aaccttgtaa
    29761 tctgctggca cgcaaaatta ctttcacatg gagtctttat ggatatcatt tctgtcgcct
    29821 taaagcgtca ttccactaag gcatttgatg ccagtaaaaa acttaccccg gaacaggccg
    29881 agcagatcaa aactctcctg caatacagcc catccagcac caactcccag ccgtggcatt
    29941 ttattgttgc cagcacggaa gaaggtaaag cgcgtgttgc caaatccgct gccggtaatt
    30001 atgtgttcaa cgaacgtaaa atacttgatg cctcgcacgt cgtggtgttc tgtgcaaaaa
    30061 ccgcgatgga cgatgcctgg ctgaagctgg ttgttgacca ggaagatgct gatggccgct
    30121 ttgccacgcc ggaagcgaaa gccgcgaacg ataaaggtcg caagttcttc gctgatatgc
    30181 accgtaaaga tctgcatgat gatgcagagt ggatggcaaa acaggtttat ctcaacgtcg
    30241 gtaacttcct gctcggcgtg gcggctctgg gtctggacgc ggtacccatc gaaggttttg
    30301 acgccgccat cctcgatgca gaatttggtc tgaaagagaa aggctacacc agtctggtgg
    30361 ttgttccggt aggtcatcac agcgttgaag attttaacgc tacgctgccg aaatctcgtc
    30421 tgccgcaaaa catcacctta accgaagtgt aattctctct tgccgggcat ctgcccggct
    30481 atttcccctc agattctcct gatttgcata accctgtttc agccgtcatc ataggctgct
    30541 gttgtataaa ggagacgtta tgcaggattt aatatcccag gttggggatt tagcgggtat
    30601 tgagatcgat cacaccacct cgatggtgat gattttcggt attatttttc tgaccgccgt
    30661 cgtggtgcat attattttgc attgggtggt actgcggacc ttcgaaaaac gtgccatcgc
    30721 cagttcacgg ctttggttgc aaatcattac ccagaataaa ctcttccacc gtttagcttt
    30781 taccctgcag gggattatcg tcaatattca ggcggtattc tggctgcaaa aaggcaccga
    30841 agcggcagat attctgacta cctgcgcgca gttgtggatc atgatgtatg cgctgctttc
    30901 agtcttctcg ttgctggatg ttattttgaa tctggcgcag aaattcccgg cggcatctca
    30961 gttaccgctg aaagggatat ttcaggggat taaactgatc ggcgcaatcc tggtcggcat
    31021 tttgatgatc tcgctgctga ttggtcagtc gccagcgatt ctgatcagcg gtcttggtgc
    31081 aatggctgcc gtgctgatgt tggtatttaa agatccgatt cttggtctgg tggcaggtat
    31141 tcagctttcc gcgaacgata tgctgaaact gggcgactgg ctggagatgc cgaaatacgg
    31201 cgcggatggc gcggtgatcg atattgggtt aaccaccgtc aaagtgcgta actgggacaa
    31261 taccattacc actattccca cctggtctct ggtttctgac tcctttaaaa actggagcgg
    31321 gatgtcagca tctggcgggc gacgtattaa gcgcagtatc agtattgatg tcaccagtat
    31381 tcgttttctt gatgaagacg aaatgcaacg tctgaataaa gcgcacttgt taaaacctta
    31441 tttaaccagc cgccatcagg aaattaatga gtggaatcgc cagcaaggtt ctacggagtc
    31501 gatattaaac ctgcgccgaa tgaccaatat tggaaccttt cgtgcctatc tgaacgaata
    31561 tctgcgtaac catccgcgga ttcgtaaaga tatgacctta atggtacgcc aactggctcc
    31621 aggtgataac ggtttaccgc tcgagatcta cgcgtttacc aacaccgtgg tgtggctgga
    31681 atatgaaagc attcaggctg atatattcga tcacatattt gcgattgtcg aagagtttgg
    31741 tctgcgactt catcagtcgc cagccggcaa tgatattcgc tctctggcgg gtgcatttaa
    31801 gcagtaatta aaaaaaccgc tctcatcgaa tggatgagag cggacatcgt tttgtcggat
    31861 gtagcgtgaa tgccttattt ccgacgcagc gttttaaatg ccacaaacag gaatacaatc
    31921 cacaccggca gcaggatcgc tgacaagcgc atatcatcca tcgtgcacat cagcagcaaa
    31981 atcatgccga ggaaggcaat gcaaagatag ttgccgaacg gataaagcag cgccttaaac
    32041 tgtgtttcac gtccctgacg tcgcatcgcc gcgcgaaaac gcagatgcgc caggcagatc
    32101 ataatccagt tcaacagcag cgttgctacc accagcgcca tcagcagacc aaacgctttt
    32161 tgcggcagca gatagttgat taacaccacc agcgaagtga tcgctccgga aagcatcagc
    32221 gagttaatcg gcacgccgcg atggctgacg cgagtcaaaa acttcggcgc attaccctgc
    32281 acagaaaggc caaacagcat gcggctgtta gagtaaaccc cgctgttata cactgacagc
    32341 gatgctacca gaatgacgaa gttcagcgca gaagctacct cgttgctgtc gagattatgg
    32401 aaaatcatca caaacgggct actgttggat ttcacttcca cccacggata gagcgccagt
    32461 aaaaccacca gtgaacagat gtaaaacagc aggatgcgat acaccacctg atttaccgct
    32521 tttggaatgc ttttttccgg atcgcgcgct tcagcggcag taatcccaat cagctccaga
    32581 ccgccgaagg agaacataat taccgccagc gacaaaatca gcccattcca gccggtggcg
    32641 aagaaaccac cgtagcgcca gaggttgtcg atactggctt tctcgccgcc gtgaccagaa
    32701 aacagcagcc acaggccaaa gccgatcata ccgatgattg ccagcacttt aatcaacgca
    32761 aaccagaact cggtttcgcc atataagcgc acgttcacca ggttaacggc gttgatgata
    32821 ataaagaagg cggcagccca aatccacgtt ggaacatccg ggaaccagta ctgcatatag
    32881 atgcccgcag cggtcagctc tgccattccc accagcacga acattaccca gtagttccag
    32941 ccagagagga agcccgcaaa cggtccccag tatttatagg caaagtgggc aaatgaaccg
    33001 gataccggct cttcgaccac catctcgccg agctggcgca taatcaggaa agcgatgatc
    33061 ccggcgacgc cgtagcccag caatacagcc ggacccgcca tctgaatcgc cgggccaatg
    33121 ccaagaaaca gaccagtacc aattgcgcca cccagcgcaa tcagttgaat atgacggtta
    33181 tgtaatccgc gatgaagcgt cggctcttga tttgacgcag tatcttccga tacggttgac
    33241 gcgtttttca cgcctttccc ctgtgtgtct tttttgttga ggggcacctt ttaacattta
    33301 gtgcccatcg tcgcaagaca caatccacac ggttaaacgg ggtatcctgc ttttatttcc
    33361 gtacccgatg tcggtgcagc cacatcagct tatacgccgc cgggataata aacagcgaca
    33421 gcaaaggcgc ggtgatcatg ccgccaatca tcggtgcggc aatccggctc atcacctctg
    33481 aaccagcccc cgttccccac agaatcggca gcagaccggc gataatcacc gccaccgtca
    33541 tcgctttcgg gcgcacgcgc agtaccgcgc cgtgatacaa cgcctcatcc agcttctgct
    33601 cactgaatgt ttgcggatta ttcaacgacg gctcggcctc tatggcgtga cgtaaataca
    33661 tcagcatcac cacgccaaat tcggcggcgc ccccggcaag ggcaataaaa ccggtgcccg
    33721 ttgccacaga aagatgaaag cccatccacc acagcaacca gatgccgccc accagcgcaa
    33781 acggtacgct gctgataatc agcaacgctt cgcccacccg acggaatgcc agatacaaca
    33841 gcacgaagat gatcatcagt gtcatcggca ccatgagttt cagcttatgg ttggcgcgct
    33901 ccagtagctc gaactgcccg gagaatgcca cgctggtgcc cggttttaac tgcacttttt
    33961 caactatcgc tttttgcaaa tcgtgaacca ccgacaccat gtcacgatcg cgggcatcga
    34021 tataaatcca gctcgtcggg cgcgcattct cggttttcag catcgacggt ccggtagaga
    34081 ctttaacgtc ggccacgtct gccagggtga tttgctgctt catcggcgtc aggatcggta
    34141 gctggcgcag tgcctgcttt gcggataacg cagattaatg ggataacggg caatcccttc
    34201 caccgtttcg ccaaccatcg ccccgcccac cgcagaagtc acaaacaact gcacatccgc
    34261 caccgtcata ccgtaacgcg cggctttttc acggttaatc tcaacgttga tatagcgccc
    34321 accttccaga cgctcggcaa gcgcagaagc cacgcctggc accgttcgcg ctacttcttc
    34381 aatttgctca gccatcgcgt cgatatccgc cagcacagtg ccggaaactt taatgccgat
    34441 ggggctttta atgccggttg agagcatatc gatacggtta cgaattggcg gcacccacag
    34501 attcgccagc cccggcagcc gcacggtgtt atccagttcc tcaatgattt tgtccatcgt
    34561 catgccaggc cgccactgat cctgcggctt aagctggatg gtcgtttcaa ccatctcgag
    34621 cggagcagaa tcggtggcgg tttccgcttt cccggttttg ccaaataccc gcgccacttc
    34681 aggtacgctc ataattagct tgtcgttttt ttgcagcata ctcgccgcct ctgctgcgga
    34741 aatccccggc agcgtcgatg gcatatacaa caagtcgcct tcattgatct gcggtaaaaa
    34801 ttccccgcca actttattga gcggccagag aaccgtcagc accgaaagcg ctgccaccaa
    34861 cagcgtggtt ttcggccagt gcagtacttt cagcaataga ggatgataaa cacgaatcaa
    34921 aaagcgattg agcgggttac tgctttccgg cggaattttg ccacggatcc agtaacccat
    34981 caggatcggg atcactacga tcgccagcag cgccgcaccc gccatcgcat acgttttggt
    35041 gaacgccagc gggccaaaca gacgcccttc ttgcccttcc agggtgaaga ttgggataaa
    35101 cgacagcgtg ataatcagca gactgataaa cagcgccggc cccacttcaa cagatgcatc
    35161 ggtaatcacc tgccagcgcg ttttattatc cagcgtggcg tcaggatgct ggtgctgcca
    35221 ctcttccagc cgtttatgcg cattctcgat catgacgata gcagcatcga ccatcgcccc
    35281 gacggcaatc gcaatgccac ccaacgacat aatattggca ttcagtccct ggaagggcat
    35341 gacaataaaa gcaatacaca accccagcgg caacgaaata atcgccacca gcgccgagcg
    35401 cacatgccag agaaacagcg cacagactac cgccaccaca ataaactctt ccagcaactt
    35461 gccgctgagg ttgtcgatgg cgcggtcaat gagctggctg cgatcgtatg ttgtaactat
    35521 ctccacgcct tccggcagac tacttttcag cgtttccagt ttgtccttca cggcggcgat
    35581 cacttctcgg gcgtttttac cggagcgcag gatcaccacc ccgcccgcca cttcgccttc
    35641 gccgttcagt tcggcaatgc cccggcgcat ctccgggcca acctggacct tcgcaacatc
    35701 gcgcagataa acgggcacgc cattttcact ggcttttaaa acgatgtgat taaagtcgtc
    35761 gagcgtttgc agatagccgc tggcgcgcac catatattcc gcttccgcca gttcgatcga
    35821 cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt tttacttcgg cgagactgat
    35881 gccatactgc gccaggcgct ggggatcgat aaccacctga tactctttca ccacaccgcc
    35941 caccgacgcc acttccgcaa cgtcagggat ggttttcagc tcatatttga gaaaccagtc
    36001 ctgtaatgag cgtaaatcgg ccagatcgtg cttaccgctg cgatccacca gtgcatattc
    36061 atagatccag ccaacacccg tggcatctgg ccccagctcg gcgctgactc ccgcaggcag
    36121 cttaccctgt acctggttga ggtactccag cacccgcgaa cgcgcccagt acggatcggt
    36181 accatcttcg aaaatgacat acacataaga gtcgccaaac tgcgagaaac cacgcacagt
    36241 cttcgcgcca ggcaccgaca acatggtggt ggttagcgga taagtcacct gattttcaac
    36301 gatttgcggt gcctgcccgg gatagctggt tttaataatc acctgcacat cggagagatc
    36361 cggcagcgca tccactggcg tattaatgat ggtccaggtg ccccagatgc taagaaacaa
    36421 cgcgcccatc agcaccaaaa aacggttcgc caccgagcga cgaataatcc attcaatcat
    36481 tggttattcc ctcaatgcgc atgggtagca ctttcagagc gcatccgctc cagtgcgcca
    36541 gaaatattgg cttcggaatc aatcaggaaa aggccgctgg aaaccacctt ttcaccttcc
    36601 gccagaccag agcgtaatgc ggtgacacct tgcgatgcct ggaaaacggc aacgcgtttc
    36661 ggtacaaagc gcccgtcggc atcaatggta atcacccgct gttcgctacc ggtatcaatc
    36721 agcccttgtg acggaatgag cagcatcggt tcgctggcgg tattgagttg caaccaggcg
    36781 ttcatgcccg gttttagcgc ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg
    36841 cgggtcgcgg catccacgcc gggtagcagc gtccatttgc ggatggtgag tgttttatcc
    36901 ggtcgtgccg gaacggtgag cgtaaactgc gaggcatctt tcaccagcca ggcgatagac
    36961 tccgggatcg cagcagtgac ccacaccggg tccataccct gaattttcgc taccacgtta
    37021 tctttggcga tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc
    37081 gctttgagcg taaagcgagt ctggattttt tgcgtggcga tcaggcggcg aatatccgcc
    37141 tccggcattc ccgccagtcg cagccgctca agaatgcctt cagtctgggt cgccgtaccg
    37201 ccggtttcgc gcagcagtaa atactcactc tgcgcttcca cccagtcagg aatggtcagg
    37261 tcgagaagcg gtgtgccctt ttgtacttta tcgcccacgg taagcggata caccttgtcg
    37321 ataaacccgg cagcg
//
LOCUS       DAJTSL010000019        37216 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTSL010000019 DAJTSL010000000
VERSION     DAJTSL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.019"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..800)
                     /locus_tag="OO433_000849"
     CDS             complement(<1..800)
                     /locus_tag="OO433_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR6049420.1"
                     /translation="MTIEKSRFSSGAAPQPNVNTGVINGNDFAAIVPVIAGRIGGREA
                     NIVSAKALHKALGVGRDFTNWIKGRIDQYGFIAGTDFIRVENLSTPKRASAKTRQQIE
                     HDYLLSLDMAKEVAMVERNEQGRAIRRYFIQCEEELQRSVPEIAARYRRQLKARLSAA
                     NNFKPMCDALNMARAEMGKTTQQRHYSNESNMIARIVLGGMTAKQWAQANGITGEPRD
                     SMNAAQLEHLAYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEV"
     gene            complement(814..1245)
                     /locus_tag="OO433_000850"
     CDS             complement(814..1245)
                     /locus_tag="OO433_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049421.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYASELERRLDIQLERIDEKNPDGIGSHLRYRFTGR
                     GDVLKVIRLVNHNADAGGYQGLSQPEIADILNLYPDNLTAA"
     gene            complement(1245..1448)
                     /locus_tag="OO433_000851"
     CDS             complement(1245..1448)
                     /locus_tag="OO433_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR6049422.1"
                     /translation="MNIDTIPTKGYIRRFRLAELLGVSVSTIDRKVRNGSLPRPVKLG
                     EKITAFDAVEIHNWLAERRGKVA"
     gene            complement(1561..2409)
                     /locus_tag="OO433_000852"
     CDS             complement(1561..2409)
                     /locus_tag="OO433_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049423.1"
                     /translation="MKKLPPAPFFRLNRAAELLEIDIDDLLIMAAAESIYLSAYLCGY
                     DGTLHSIASLYRNCSLFPDVKEDCLLLADIITEVEDEKSFKAGVVNHIDCHVLGLWHV
                     NASYILNIAGLDSIDTPARIFSPAHRKRELDVSANYYFFLDYTKHAFHEISVEDLYIT
                     RPWVEYIGECMDKNQPIYSPETRINLQALYEDVKNVEHGNRERHRQKREICLLALIYV
                     KRHYPEECKGKNGKETNEAWANATINHWALLGGGYDEPSIDYLKKLIADADRTPEERT
                     TAGKKR"
     gene            complement(2505..3764)
                     /locus_tag="OO433_000853"
     CDS             complement(2505..3764)
                     /locus_tag="OO433_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6049424.1"
                     /translation="MPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
                     YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAE
                     KNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRD
                     ELIQLFKAMRTAKGFSRQNEITFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPLRRAN"
     gene            complement(3955..4818)
                     /locus_tag="OO433_000854"
     CDS             complement(3955..4818)
                     /locus_tag="OO433_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6049425.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDISAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            4945..5661
                     /gene="rph"
                     /locus_tag="OO433_000855"
     CDS             4945..5661
                     /gene="rph"
                     /locus_tag="OO433_000855"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6049426.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            5727..6368
                     /gene="pyrE"
                     /locus_tag="OO433_000856"
     CDS             5727..6368
                     /gene="pyrE"
                     /locus_tag="OO433_000856"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6049427.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(6405..7001)
                     /gene="slmA"
                     /locus_tag="OO433_000857"
     CDS             complement(6405..7001)
                     /gene="slmA"
                     /locus_tag="OO433_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6049428.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(7108..7566)
                     /gene="dut"
                     /locus_tag="OO433_000858"
     CDS             complement(7108..7566)
                     /gene="dut"
                     /locus_tag="OO433_000858"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6049429.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(7544..8764)
                     /gene="coaBC"
                     /locus_tag="OO433_000859"
     CDS             complement(7544..8764)
                     /gene="coaBC"
                     /locus_tag="OO433_000859"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6049430.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEGLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSENNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            8936..9604
                     /gene="radC"
                     /locus_tag="OO433_000860"
     CDS             8936..9604
                     /gene="radC"
                     /locus_tag="OO433_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6049431.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            9821..10057
                     /gene="rpmB"
                     /locus_tag="OO433_000861"
     CDS             9821..10057
                     /gene="rpmB"
                     /locus_tag="OO433_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6049432.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARSEKY"
     gene            10078..10245
                     /gene="rpmG"
                     /locus_tag="OO433_000862"
     CDS             10078..10245
                     /gene="rpmG"
                     /locus_tag="OO433_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6049433.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            10343..11152
                     /gene="mutM"
                     /locus_tag="OO433_000863"
     CDS             10343..11152
                     /gene="mutM"
                     /locus_tag="OO433_000863"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6049434.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(11191..11670)
                     /gene="coaD"
                     /locus_tag="OO433_000864"
     CDS             complement(11191..11670)
                     /gene="coaD"
                     /locus_tag="OO433_000864"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6049435.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(11678..12955)
                     /gene="waaA"
                     /locus_tag="OO433_000865"
     CDS             complement(11678..12955)
                     /gene="waaA"
                     /locus_tag="OO433_000865"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6049436.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQVLLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            13368..14426
                     /gene="rfaQ"
                     /locus_tag="OO433_000866"
     CDS             13368..14426
                     /gene="rfaQ"
                     /locus_tag="OO433_000866"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6049437.1"
                     /translation="MDKLFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLRVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            14423..15547
                     /locus_tag="OO433_000867"
     CDS             14423..15547
                     /locus_tag="OO433_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6049438.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     YPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            15540..16337
                     /gene="rfaP"
                     /locus_tag="OO433_000868"
     CDS             15540..16337
                     /gene="rfaP"
                     /locus_tag="OO433_000868"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6049439.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWSAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            16353..17369
                     /gene="waaO"
                     /locus_tag="OO433_000869"
                     /gene_synonym="rfaI"
     CDS             16353..17369
                     /gene="waaO"
                     /locus_tag="OO433_000869"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6049440.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRFTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            17386..18381
                     /locus_tag="OO433_000870"
     CDS             17386..18381
                     /locus_tag="OO433_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCR6049441.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            18391..19083
                     /gene="rfaY"
                     /locus_tag="OO433_000871"
     CDS             18391..19083
                     /gene="rfaY"
                     /locus_tag="OO433_000871"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6049442.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            19109..20134
                     /locus_tag="OO433_000872"
     CDS             19109..20134
                     /locus_tag="OO433_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCR6049443.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCNFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            20219..21202
                     /locus_tag="OO433_000873"
     CDS             20219..21202
                     /locus_tag="OO433_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6049444.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIVNKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNEKELRDCNLPGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            21248..22501
                     /locus_tag="OO433_000874"
     CDS             21248..22501
                     /locus_tag="OO433_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6049445.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQYLLDK"
     gene            complement(22571..23542)
                     /gene="rfaC"
                     /locus_tag="OO433_000875"
     CDS             complement(22571..23542)
                     /gene="rfaC"
                     /locus_tag="OO433_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6049446.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFDYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(23546..24592)
                     /gene="rfaF"
                     /locus_tag="OO433_000876"
     CDS             complement(23546..24592)
                     /gene="rfaF"
                     /locus_tag="OO433_000876"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6049447.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNTLLLQEEA"
     gene            complement(24602..25534)
                     /gene="rfaD"
                     /locus_tag="OO433_000877"
     CDS             complement(24602..25534)
                     /gene="rfaD"
                     /locus_tag="OO433_000877"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6049448.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            25748..26944
                     /gene="kbl"
                     /locus_tag="OO433_000878"
     CDS             25748..26944
                     /gene="kbl"
                     /locus_tag="OO433_000878"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6049449.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            26954..27979
                     /gene="tdh"
                     /locus_tag="OO433_000879"
     CDS             26954..27979
                     /gene="tdh"
                     /locus_tag="OO433_000879"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6049450.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            28218..29252
                     /gene="waaH"
                     /locus_tag="OO433_000880"
                     /pseudo
     CDS             28218..29252
                     /gene="waaH"
                     /locus_tag="OO433_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(29239..30198)
                     /locus_tag="OO433_000881"
     CDS             complement(29239..30198)
                     /locus_tag="OO433_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6049451.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPLTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(30202..31485)
                     /gene="envC"
                     /locus_tag="OO433_000882"
     CDS             complement(30202..31485)
                     /gene="envC"
                     /locus_tag="OO433_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6049452.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQCGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(31495..33039)
                     /gene="gpmM"
                     /locus_tag="OO433_000883"
                     /gene_synonym="pgmI"
     CDS             complement(31495..33039)
                     /gene="gpmM"
                     /locus_tag="OO433_000883"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6049453.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            33284..33715
                     /locus_tag="OO433_000884"
     CDS             33284..33715
                     /locus_tag="OO433_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6049454.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            33857..34108
                     /gene="grxC"
                     /locus_tag="OO433_000885"
     CDS             33857..34108
                     /gene="grxC"
                     /locus_tag="OO433_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6049455.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            34171..34638
                     /gene="secB"
                     /locus_tag="OO433_000886"
     CDS             34171..34638
                     /gene="secB"
                     /locus_tag="OO433_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6049456.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            34638..35657
                     /gene="gpsA"
                     /locus_tag="OO433_000887"
     CDS             34638..35657
                     /gene="gpsA"
                     /locus_tag="OO433_000887"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6049457.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGAEPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            35737..36558
                     /gene="cysE"
                     /locus_tag="OO433_000888"
     CDS             35737..36558
                     /gene="cysE"
                     /locus_tag="OO433_000888"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6049458.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(36611..37084)
                     /gene="trmL"
                     /locus_tag="OO433_000889"
     CDS             complement(36611..37084)
                     /gene="trmL"
                     /locus_tag="OO433_000889"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6049459.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
BASE COUNT         9222 a         8859 c         9269 g         9866 t
ORIGIN      
        1 tttacctcca gacgtttagc gagccagcgt tgcgacaggc gggtgagttc tcctttgcgc
       61 tgctcatatt ccatgcccat atcaattaac gtgatattgg tgctttcgag ataggcgagg
      121 tgttcaagct gtgctgcgtt catgctgtcc cgtggttcgc cagtaatgcc atttgcctgc
      181 gcccactgct tcgccgtcat gccacccagc acgatacggg cgatcatgtt gctctcgttg
      241 ctgtagtggc gctgctgcgt cgttttgccc atctcggcac gggccatatt cagcgcatcg
      301 cacattggct taaagttgtt tgcggcactg agacgggctt ttagctggcg acgatagcgg
      361 gcggcgattt caggcacgct acgctgtaat tcttcctcgc actggatgaa ataacggcgg
      421 atagcgcggc cctgctcgtt gcgttcaacc attgccactt ctttagccat atccagcgat
      481 agcaggtaat catgctctat ttgctggcga gtttttgcgc tcgcccgttt tggcgtgctc
      541 aaattttcaa cacggataaa atcggttccg gcaataaaac cgtactggtc aattcggcct
      601 ttaatccagt tagtaaagtc gcggcccacc cccagcgctt tatgcaacgc cttagcgctc
      661 acaatattgg cttcacgccc gccaatgcgt ccggcaataa cgggaacaat agcggcaaag
      721 tcgttgccgt taattactcc cgtgttaacg ttgggttgcg gggcggcccc cgaactgaat
      781 cggctttttt cgatagtcat attttcggct ccgttatgcg gcggtgagat tgtccgggta
      841 tagattgaga atgtcggcaa tttcaggctg agaaagtccc tgatagccgc cagcgtcggc
      901 gttatggttc acaagccgaa taacttttag aacgtcaccg cgcccggtaa agcgatagcg
      961 taaatgtgac cctatcccgt cggggttctt ttcgtctata cgctcaagct gaatatcaag
     1021 cctgcgctct aactcgctgg cgtagttcct tccagacgaa aggcggcaat aacgcagaat
     1081 gtcattttct gtccatccct ctataccagt acgcagcata tagactctgg cacgatgctt
     1141 tttcggtgtg cgctttggtg cttgaaggga ttttgtaagg ggcgtaacat cagatacgcg
     1201 aatatctgaa ttagccgcct gtaatgggcg gtttttcttt tccatcaggc caccttcccg
     1261 cggcgttctg ccagccagtt gtgaatctca accgcatcaa acgcagttat tttttctccc
     1321 agctttaccg gacgtggtag tgaaccgttt cttactttac ggtcgatggt ggatacacta
     1381 acgcccagca attcagccag gcggaagcgt ctgatatagc cttttgtagg gatagtgtcg
     1441 atgttcatga ttcccgtaac ctcttagcga tagttgttgc ttcgttacgg gtatttaatg
     1501 gcgtgcacgc tcaaaaagga atgatcgtat tttttactat cgtgatagta aaatttacta
     1561 tcaccttttt ttccctgccg tggtgcgttc ttccggtgtt ctgtctgcgt cggctatgag
     1621 tttcttcagg taatctatgg atggctcatc atagccgccc cccaaaagcg cccagtggtt
     1681 tattgtggcg ttcgcccatg cttcgttggt ttccttcccg ttcttccctt tacactcttc
     1741 tgggtagtgc cgcttcacat aaatcaacgc caagaggcaa atttcgcgct tttgcctgtg
     1801 gcgctctctg tttccgtgct caacgttttt aacatcctca taaagagctt gtaagtttat
     1861 gcgagtttcg gggctgtata tgggctggtt tttgtccatg cattcgccga tatattcaac
     1921 ccacggcctt gttatgtaca aatcctctac ggatatttcg tggaaggcat gttttgtata
     1981 atcgagaaaa aaataataat tcgcacttac gtctagttct ctttttctgt gtgcggggct
     2041 gaatatgcgt gcgggagtgt caatagaatc aagccctgcg atgttgagta tataggaggc
     2101 gttaacatgc catagcccaa gcacatggca atctatatga tttacaacgc ctgccttaaa
     2161 gcttttttcg tcctctactt cagtgattat gtcggcaagt aataagcagt cttctttgac
     2221 gtctgggaat aaagagcaat tgcggtaaag actcgcgatg ctatgtaatg tgccgtcgta
     2281 gccgcaaagg tacgcgctca aatatataga ttctgccgcc gccataatca aaagatcatc
     2341 aatatctatt tctaataatt cggctgctct gttaagcctg aaaaaagggg cgggtggtaa
     2401 ttttttcatt gtgtcacctt ttacgctgcg ccttattttg tgattgagcc aggcggtaag
     2461 gcttcccgct tttcgccccg tcgagctagg ctcaatctat tcagttagtt cgcccttctg
     2521 agaggcgtta cgttgtagtc ttttccgctt tccagtgcca ccagcagatc agcccactgt
     2581 gccagtgctg ctttacgctc atcaaaatac tgatggcggt tataaatccc ctctacgccc
     2641 ttaatgcgat gattgaggca tcgttccgcc acaacaggat caacacccaa cgctgctaaa
     2701 tgagtacgtg cggtgcgtcg aaagtcgtga atcgtgaaat taggcacatc cggcatttcg
     2761 gcgcgaacct tagccagtgc tacgggtaaa gtgctttcct gaatatgtgg gatcattctg
     2821 ttttgcatct tcctggccgg aagcacccat gcgctattac atgaaaaggt gtgtagctct
     2881 ctcaaccatt caacggctgg ggaaggtaga ggtatatcaa tagggtctcc gtttttgctg
     2941 cgttcttccg gcaaatgcca taccgcacca tctaaatcaa actcttccca tcgtgcggcg
     3001 cataattcca ttttgcggac gcataacgct aacagtaatt tgaacgtgat ttcgttctgg
     3061 cgactgaatc ccttagccgt gcgcatggct ttgaatagct ggattaactc atcgcgggtt
     3121 aaccagcggt cacgggcagc ttctttccct ccggcgtctg ccacctcaaa ggctgaacag
     3181 gggttaatct ctagcgcgtg ccgtttgatt ccgtagtcga atatgcggcg cgtccatctc
     3241 agcacgtccg ttgctatggt cggcgctcca cggtcaacaa tacctttcag catgtcatcg
     3301 atatggcgcg gtttcacgtc ctctaccttc atgctgccaa tgcaggggtt tatatctttg
     3361 tcgatacggc ggcggagtat atcggggtgc ttccagcgcg ggaggatctg acgctcaaag
     3421 tattcagcgg caagctctga aacgcgcata gcgttcttct ctgcttccat cttcgccagt
     3481 gcttcggttt tgcgcttctg cttctctcct gcaacgtcat agcccagcgc aacgcgagcc
     3541 gatagctctt tggctgtctc tctggccttt gatagtgata gctcgctata cgaaccaatt
     3601 agcatggcgc gggacttccc tgcgagttta tagcggaatc tccatgtggg ggttttgtcg
     3661 gcttcacggt aacgtaggta taaaccgtta ccgtctgccc gcccctcgaa tcgctctccg
     3721 ctcttaatcc atgcgcggat ctgcatgtct gttagttttg gcattttgcg aatatccaaa
     3781 ctttgaatag ggggtgtacc caaagcaatg tacccaaacc tcattgggta cacctttgtg
     3841 tacaccataa tcatgcgctt tgttgcgtct ttatgatagc ccatgataac aaaaaagccc
     3901 gctgggttgc gggcttggtg tgatttatgc gtcgtcctaa atgttgctga tagcttactc
     3961 gatattctga atctgctcgc gcatctgctc aatcaacact ttcagctcaa tcgcggagtt
     4021 tgtcacttcg gcattgatag acttcgacgc aagagtgttc gactcgcggt tgaattcctg
     4081 catcataaag tccagacgac gaccaacggc ttcttttttc ttcagaatgt tgtaggtctc
     4141 tttgacatgc gcttccaggc ggtccagttc ttcggcaacg tcaattcgtt gtgccagcag
     4201 aaccagctcc tgctccagac ggttgttttc caactgaacc tgagcatctt ccagcttcgc
     4261 gaccagacgc tcacgctgcc attgcaggat ttccggcata tgggcgcgga ctttgaccac
     4321 ttcggcggtg acgccttcca gacgctgctc gatcaatgct ttcagtgcct gaccttcggt
     4381 ttcgcgcgcg acaataaagt cgtccagcgt accatccagc gccgcgagaa tttcagcggc
     4441 aatggcgtca agatcctgct cctgggctgc catcacgccc ggccagcgta gaatatcaac
     4501 cgggttgatt tccccttcgt cactctgcat ttttacccag ttcgcggcag ttaccagctg
     4561 tttagccagt ttttcgttga ggatcagctc accttgcgcg ctaatatctg gctcatagcg
     4621 cagggtacat tccactttac cgcgcgtcag gcgagaacga atacgctcgc gaacgacagg
     4681 ttcaaggcta cggaactgct ccggcagacg aaagtaagtt tccagataac gctggtttac
     4741 cgagcgcatt tcccaggttg cgctccccca ttcacccttg atttcacgcc gggcgtaggc
     4801 ggtcatactg cggatcatag acgttcctgt ttataaaagg agaggtggaa ggattatagc
     4861 catcgatgcc ttgtcaggat aggaataacc gccggaagtc cgtataatgc gcagccacat
     4921 ttgtttcaag ccggagattt caatatgcgt ccagcaggcc gtagcaataa tcaggtgcgt
     4981 cccgttaccc tgactcgtaa ctatacaaaa catgcagaag gctcggtgct ggtcgaattt
     5041 ggcgatacca aagtgttgtg taccgcctct attgaagaag gcgtgccgcg cttcctgaaa
     5101 ggtcagggcc agggctggat caccgcagag tacggcatgc tgccacgttc tacccacacc
     5161 cgtaacgctc gtgaagcggc gaaaggtaag cagggtggac gcacaatgga aatccagcgt
     5221 ctgatcgccc gtgctcttcg cgcggcagta gatttgaaag cgctgggtga gttcaccatt
     5281 acgctggact gcgacgtgct tcaggctgat ggtggcacgc gtaccgcgtc gattacgggt
     5341 gcctgcgtgg cgctggcaga tgcgctacag aagctggtgg aaaacggcaa gctgaaaacc
     5401 aacccgatga aagggatggt agccgcagtt tctgtcggaa ttgtgaacgg cgaagcggtt
     5461 tgcgatctgg aatacgttga agactctgcc gcagagaccg acatgaacgt agtgatgacc
     5521 gaagacgggc gcatcattga agtgcagggg acggcagaag gcgagccgtt cacccatgaa
     5581 gagctactca ccttgttggc tctggcccga gggggaatcg aatccattgt agcgacgcag
     5641 aaggcggcgc tggcaaactg atttttaagg cgactgatga gtcgcctttt ttttgtctgt
     5701 agaaaagtaa gatgaggagc gaaggcatga aaccatatca gcgccagttt attgaatttg
     5761 cgcttggcaa gcaggtgtta aagtttggcg agtttacgtt gaagtccggg cgcaaaagcc
     5821 cctatttctt caacgccggg ctgtttaata ccgggcgcga tctggcactg ttaggccgtt
     5881 tttacgctga agcgttggtg gattccggcc ttgagttcga tctgctgttt ggccctgctt
     5941 acaaagggat cccgattgcc accactaccg ccgtggcgct ggcggagcat catgaccttg
     6001 acctgccgta ctgctttaac cgcaaagagg caaaagacca cggtgaaggc ggcaatctgg
     6061 ttggtagcgc gttacaagga cgcgtaatgc tggtagatga tgtgatcacc gccggaacgg
     6121 cgattcgcga atcgatggag attattcagg ctaatggcgc gacgcttgct ggcgtgttga
     6181 tttcgcttga tcgtcaggaa cgggggcgcg gcgagatttc ggcgattcag gaagttgagc
     6241 gtgattacaa ctgcaaagtg atctctatca tcaccctgaa agacctgatt gcttacctgg
     6301 aagagaagcc ggaaatggcg gaacatctgg cggcggttaa ggcctatcgc gaagagtttg
     6361 gcgtttaaag aaactcgccg gatgaaaagt catccggcgt catattactg caactgtgcc
     6421 gcaattagcg gccagcgggc gtcaaaatca tccgtcgggc ggtatttaaa ttcgctgcgg
     6481 acaaaacgtg acagcatacc ttcacagaag gccaggatct ggcttgccag cagggtttca
     6541 tcggtggtgt aaccttcacc ctcacgcatt ctcttttcac gcaatacctg acgcagctgc
     6601 gcttcaatac gctcgaacag ctggttgatg cgcccttgca ggcgatcctg ttcaaacatt
     6661 agcgcatgac cagtgaggat gcgggtcagg ccaggattac gctcaccaaa accgagaagc
     6721 agcaacacaa tcagacgcag gcgcgctgtg gtgtctttct catctttcag aatcaggttg
     6781 atgcgagtaa tcaggctatc ttcgataaac tcaatcaggc tatcgaacat gcgggtctta
     6841 ctggggaagt ggcgatacag tgccgcttcg gaaacgccga cagaggcggc cagttttgcc
     6901 gtcgtgatac gttggcttcc atcgctggat tccagcatca gcgccagaga ctgaagtatt
     6961 tcctcgcgac ggttcctttt cgcagtttgt ttttctgcca tgttacaaaa tacccctgaa
     7021 aataagcact tgccaggcgg cacccacgct atgaccgcaa acgaaatgtt tgcggctatg
     7081 ttatgacgtt attcggatgc gtatgtgtta ctgacgacca gagtgaccaa agccgccttc
     7141 accgcggtcg gtggcgtcga aatcttccac cagattaaat tcagcctgta ctaccggaac
     7201 aaaaatcatc tgggcgatgc gttcgccagg ttgaatggtg aagctgtcct gaccacggtt
     7261 ccacacggaa atcatcaact ggccctgata gtcggaatcg atcaatccta ccaggttacc
     7321 aagcacgata ccgtgcttat gtcccaatcc ggagcgcggc agcatcattg ccgccagtga
     7381 aggatcggca atatgaatcg ccagcccggt cggaaccagc gtagtgtcac ccggagccag
     7441 ttctacggcg tcgtcgagac aggcacgcag gtcaagtccg gcagagccag aggtggcata
     7501 agtcgggagc ggaaattcct tcccaacgcg cgggtccaga atcttaacgt cgattttttt
     7561 catcataacg ggtcacgatc tcgtcgagta atagttggcc aaggagctct ttgcgctcaa
     7621 gcggtaagac tttatctccg tcctgccaga aaaggtgtaa tgcgttgttt tcgctgttaa
     7681 atccttgagt tggctgggaa acatcgttcg cgcagatcag atcaaggttt ttacggatac
     7741 gtttttgccg ggcgtattct tccacattat ttgtttcggc ggcaaatcca acgacgtagg
     7801 gtcgatggtc ttttagtgcg gcaacgcctg cgacgatatc ggggttttta accattttta
     7861 ttgttaattc atcaccctgc gtggcctgct ttttgatttt ttctggggcc acggtagctg
     7921 cgcgataatc cgccacggcg gcgcagccga taaaaatatt ttgctgctgt acagaagcat
     7981 tcacggcggc ttccatttcc agcgcggtca tcacatcaac acgtttaaca aacggtggcg
     8041 tcggtagtga aaccggacct gataccagcg tgacgttcgc gccacgacgg gcagcggcgg
     8101 cggcgatagc aaaacccatc ttgccggagc tgtgattaga gatataacgc acgggatcga
     8161 gcggttcacg cgtcggaccg gcggtaatca taatgttcag atgtttcagg tcgttgacgg
     8221 gcgaaaaatg cgccaccgcc atatccacaa tggttaacgg atcgagcatt cgcccaggac
     8281 cgatatcacc acaagcctga ctgccactgt ctggccccca gatgagcaaa ccacgggaag
     8341 caagcccctc taaattatgc tgcgtggcag cggcacggta catctgctgg ttcatggcgg
     8401 ggagcacggc tacaggcgca ggtgtagcca gacaaatcgt cgataccagg tcattcgcca
     8461 ttccggcagc aacacgggca atcaaatctg ccgtggcagg ggcgagaatc actaaatcag
     8521 cccatttacc cagctcaata tggcccatag cggcttcggc tgccgggtcc agcaaactgt
     8581 cggaaacggg ataaccagaa accgcctgca agctaagtgg ggtgataaag gcttttgccg
     8641 cttcggtcat ggctacgcgg acgtcggccc cgcgatcgcg caaacgacgc accagttcag
     8701 gggttttata ggcagcaata ccgccgctaa cgccgagaac gattttttta ccggccaggc
     8761 tcatgatgat ttttcctgtt gggtgacacc agaagttggc gattttatca caatactttt
     8821 gttgtcgtgc cttcacctga gaatcacttt gcgaggcgct ttccaggatt gaaaactggc
     8881 cgtcgattta acggaacggc tatgacagga tgcgagcacc acaaaggagg tgaaggtgaa
     8941 aaacaattca cagctgttga tgccgcgcga aaaaatgctg aagtttggta ttagcgcctt
     9001 aacggatgtc gagctgctgg cgctatttct gcgtaccgga acgcgcggta aagatgtatt
     9061 aaccctggca aaagagatgc tggagaattt cggctctctt tatggcttgt taacctctga
     9121 atatgaacaa tttagtggcg ttcatggaat tggcgtggcg aaatttgccc agttaaaggg
     9181 gattgctgaa ctggcgcggc gttactacaa cgtgcggatg cgtgaagaaa gccctttact
     9241 cagcccggag atgacgcggg aatttttaca aagccagctt acgggtgagg agcgggagat
     9301 ctttatggtg atctttctcg actcacaaca ccgggttata acgcatagcc gtcttttttc
     9361 cggcacgcta aaccatgttg aagtccatcc tcgggaaatt atccgcgaag cgataaaaat
     9421 aaacgcctcg gcgctgatcc ttgcacataa tcacccttcg ggttgtgctg aacccagtaa
     9481 agcggataaa ctcattactg aacggataat aaagagttgt cagttcatgg atttacgcgt
     9541 gctcgaccat atcgtgattg ggcgtggaga gtatgtttct tttgccgaac gcggctggat
     9601 ttaacccgct atgcgcgatc cttcgggatc tttgtctgtt cgggacttga gcacatcgct
     9661 gagtcagcgt atactacgcc acctttgaga atctcgggtt tggcatttgg gcctggcaat
     9721 cgagagttca catagaactg cgatgactgg gctgtaaagc ctgacgaggc gccaataccc
     9781 catacgaagc tcgagctaat ttgatttttg gagaatagac atgtcccgag tctgccaagt
     9841 tactggcaag cgtccggtga ccggtaacaa ccgttcccac gcactgaacg cgactaaacg
     9901 ccgtttcctg ccgaacctgc actctcaccg tttctgggtt gagagcgaga agcgttttgt
     9961 caccctgcgc gtatctgcta aaggtatgcg tgtaatcgat aaaaaaggca tcgatacagt
    10021 tctggctgaa ctgcgtgccc gtagcgaaaa gtactaagta cttagaggaa ataaatcatg
    10081 gctaaaggta ttcgtgagaa aatcaagctg gtttcttctg ctggtactgg tcacttctat
    10141 accactacga agaacaaacg tactaagccg gaaaaactgg aactgaaaaa attcgatcca
    10201 gttgttcgcc agcacgtgat ctacaaagaa gcgaaaatca aataattctc gctttgatgt
    10261 aacaaaaaac ctcgctccgg cggggttttt tgttatctgc ttgcccccat attgactgca
    10321 tctgttcatt cctggagatg ctatgcctga attacccgaa gttgaaacca gccgccgcgg
    10381 catagaaccg catctcgttg gtgcaaccat tcttcatgca gtggtgcgca acggacgctt
    10441 gcgctggccg gtttcagaag agatctaccg tttaagcgac caaccagtgc ttagcgtgca
    10501 gcggcgggct aaatatctgc tgctggagct gcctgagggc tggattatca ttcatttagg
    10561 gatgtctggc agcctgcgca tccttccaga agaacttccc cctgaaaagc atgaccatgt
    10621 ggatttggtg atgagcaacg gcaaagtgct gcgctacacc gatccgcgcc gctttggtgc
    10681 ctggctgtgg accaaagagc tggaagggca taatgtgctg gcccatcttg gaccggagcc
    10741 gcttagcgac gatttcaatg gtgagtatct gcatcagaag tgcgcgaaga aaaaaacggc
    10801 gattaaaccg tggctgatgg ataacaagct ggtggtaggg gtagggaata tctatgccag
    10861 cgaatcactg tttgcggcgg ggatccatcc ggatcggctg gcgtcatcac tgtcgctggc
    10921 agagtgtgaa ttgttagctc gggtgattaa agcggtgttg ctgcgttcga ttgagcaggg
    10981 tggtacaacg ctgaaagatt ttctgcaaag tgatggtaaa ccgggctatt tcgctcagga
    11041 attgcaggtt tacgggcgaa aaggtgagcc gtgtcgggtg tgcggtacgc cgattgtggc
    11101 gactaaacat gcgcagcggg caacgtttta ttgtcggcag tgccagaagt aattcatgcg
    11161 cgccggatcg cataccatcc ggcataaacg ctacgctaac ttcgccatca gcgcctgatg
    11221 gacattctcc ggcaggaaat gggtgacatc gccctgatgg cgcgccacct ctttcaccaa
    11281 cgatgaagag ataaacgacc actctttcga cggcatcaga aacacacttt ccagttccgg
    11341 cattaagtgg cgattcatat gcgccagctg catttcatat tcaaaatccg ccaccgcacg
    11401 taggccacga atcagcaccg tagcgtgttg attacgggcg aagttcgcca tcaaatcact
    11461 aaacccaact acttccacgt tccccagatg cgcggttgcc tgctgtgcca gcgccacacg
    11521 ctcttccagg gtaaacatcg gttttttact ggggctggcg gcaatcgcca gaataacgtg
    11581 atcgaacatt tgcgtggcgc gcgtcacgat atcgatatga ccattggtaa tgggatcgaa
    11641 agtacccgga taaatcgccc gtttttgcat aacaacctca atgcgttttc ggtggcaggt
    11701 aaggttccag cagttgaagc aggcgctgta gcgcgccctg gttttgatac agtacttcaa
    11761 cggcatgacg gccatagaaa ctacggtaat cggcgtcggt gagtaaagag gaaacctctt
    11821 ttgcaagcgt agtggcatcg gtaacggtaa tcagcccgct tgcctgctcc agccgcgcgc
    11881 aaatgtcttt aaaattaaaa gtatgcggcc ccatcaatac cggaatagcg tgtgcggcag
    11941 cttccagcgg attatgccca ccacgttcaa ccagtgaacc gccaacaaag gcgagatcgg
    12001 caatgccgta cagtaacatc aactcgccca tcgtatcgcc aaccacaacc tgcgtgctgg
    12061 tggaggggac ttcccctgaa gagcgtgtga tatagcttag tccagcctgg cggacaaggt
    12121 taatcgcatc cgggaagcgt tctggatgac ggggtaccag gatgagcaat aaattcggga
    12181 attgctgtaa caatacctga tgtgcggcga tcaccacact ctcttcacct tcgtgagtgc
    12241 tggtggcaat ccataccggg cggtgtggtg cccactggcg gcgcagcgtc acggctttag
    12301 cagccaactg cggcgttaca gaaatatcga atttcaggct accggtaacg gttacctggt
    12361 tattttttgc gcccagcgcc acaaaacgtg caccatcttc ttcattttgg gcggcaatca
    12421 gcgtaatacg acgcagcaag cgacggacga atttacccag tttggcataa cctgcggccg
    12481 agcgggcaga gagtcgcgcg ttcgcgatca ccagcggaat tttacgttta tgtagcgccg
    12541 caatcaggtt aggccatagt tcggtttcca taatcaacac cagtttaggg tcgactttat
    12601 tcaggaaacg attaagtgca tcgggcaggt catacggcag ataaacgtgc tgaacatcct
    12661 tcccgaaagc cgattgtacg cgctccgaac cggttggcgt catggttgta acggtaatcg
    12721 gtaaatcagg ataacgatga cgcagcgcgc gcaccaacgg gatcgccgcc agagtttcac
    12781 cgacggagac ggagtgcagc ataatgccgc ctggttttag cggatggcgg taaaaaccgt
    12841 aacgttcacc ccagcgtttt cgataggccg gagccttacg tccgcgcacc cagagccgta
    12901 tccagatcag cggctgaata aggtagagaa gggcggtgta aagcaattcg agcatagtaa
    12961 atagctgact tatggatgtg ctggggattc tatgtattta gctgtggctt taccattact
    13021 tttcccgttt ttgacttaaa tagcaattta cccgccaatt cttgctgaac atctatactt
    13081 tttttgtatt tttatgcgat tcattgattt ctgctgtacg tgcaggaagg aatcgcagag
    13141 caacagtgaa agaatatggt tgctaatgat tttatttgat tgatttcttt attgaaaatc
    13201 aacaatatgc ttaaaaatca ttcgggtcgg attgccgcga aagaagtgat acactagcgc
    13261 gtcaatatca gtgcggtatc agtattcagg tagctgttga gcctggggcg gtagcgtgct
    13321 ttttctgctt caccttaatc ggataatctc aacaaaaaga gttacttgtg gataagctat
    13381 ttcgaagaat tttgctcatt aagatgcgtt ttcatgggga tatgttatta actactcccg
    13441 tcattagttc gctgaaaaaa aattaccctg acgcaaaaat cgatgtgctg ctttatcagg
    13501 acaccatccc gatcctgtct gaaaatccag agattaacgc gctctacggc ataaaaaata
    13561 aaaaagcaaa agcctcagaa aaaattgcca acttttttca tctcatcaag gtattacgtg
    13621 ccaataagta tgaccttatc gtcaatctca ccgatcaatg gatggttgct atactggttc
    13681 gcttattaaa tgcccgtgtg aaaatttccc aggattatca tcatcggcag tctgcttttt
    13741 ggcgtaaaag tttcacccat ttggtgccgt tgcagggtgg aaatgtggtg gaaagtaact
    13801 tatccgtgct gaccccattg cgagttgatt cgttggtgaa gcagacaacc atgagttacc
    13861 cgcctgcaag ctggaaacgt atgcgtcgcg aacttgatca tgctggtgtt ggacaaaatt
    13921 atgtggttat ccaacctacg gcgcggcaaa tcttcaaatg ctgggacaac gccaagtttt
    13981 ccgctgtgat tgatgcctta catgctcgtg gttatgaagt tgttctgacg tccggcccag
    14041 ataaagacga tctggcctgc gtcaatgaaa ttgcgcaggg atgccagacg ccaccagtaa
    14101 cggcgctggc tggaaaggtg accttcccgg aacttggtgc gttaatcgat catgcgcagc
    14161 tgtttattgg cgttgattcc gcaccggcgc atattgccgc tgcagttaat acgccgctga
    14221 tatcgctgtt tggtgcgaca gaccatattt tctggcgtcc ctggtcaaat aacatgattc
    14281 aattctgggc gggagattac cgggaaatgc caacgcgcga tcagcgtgac cgaaatgaga
    14341 tgtatctttc ggttattccg gcggcagatg tcattgctgc tgtcgataaa ttactgccct
    14401 cctccacgac aggtacgtcg ttatgatcgt tgctttttgt ttatataaat attttccctt
    14461 tggcggtttg cagcgcgatt ttatgcgtat tgctcagaca gtcgccgccc gaggtcatca
    14521 tgttcgggtt tatacccagt cgtgggaagg cgaataccct gatgtatttg aactgatcaa
    14581 agtgccggtt aaatcgcata ccaatcacgg gcgcaatgcg gagtattttg cctgggtgca
    14641 aaaacattta cgcgaacatc ccgtcgataa agtcgttgga ttcaacaaaa tgccggggct
    14701 ggacgtttat tatgccgctg atgtttgtta tgccgagaaa gtcgcgcagg aaaaaggctt
    14761 tttctatcac ctgacgtcac gttatcgcca ttatgccgcc tttgagcggg caaccttcga
    14821 acagggcaag ccgacacagc tgctgatgct gacagataag caaatcgccg atttccagaa
    14881 acattatcag actgaagcgg agcgttttca tattctgcca ccggggattt atcctgatcg
    14941 taaatatagc cagcagccag ctaatagccg tgaaatcttc cgtaagaaga atggaataac
    15001 cgaacaacaa tatttattgt tgcaggtcgg ttcagacttc acgcgtaaag gtgtcgatcg
    15061 ttccattgaa gcacttgctt cgttaccgga ttcgctgcgc cacaacacat tgctatatgt
    15121 tgttggacag gataaaccgc gaaaatttga ggcgctggca gaaaaacgcg gcgtgcgcag
    15181 taatgttcac ttcttctcgg ggcgcaacga tgtctcggaa ttaatggcgg cggcggattt
    15241 attactgcat cctgcctacc aggaagcagc gggaattgtg ctactggaag cgattactgc
    15301 aggattaccg gtactaacaa cagccgtttg tggctatgcg cattatattg tcgacgccaa
    15361 ttgcggcgag gctattgctg agccattccg ccaggaaaca ttgaatgaga ttttacgcaa
    15421 agcgttaacg caatcttcat tgcgccaggc ctgggcggaa aatgcgcgac attatgctga
    15481 tacacaagat ttatacagtc tgccagagaa agcggcggat atcataacgg gtggtctgga
    15541 tggttgaact taaagagccg tttgccacgt tatggcgcgg taaagatcct tttgaggaag
    15601 ttaaaacctt gcagggtgag gtttttcgtg aactggaaac tcgccgcact ctgcgatttg
    15661 aaatggcggg caaaagctat tttctcaaat ggcatcgcgg cacgaccctg aaagagataa
    15721 tcaaaaattt actctcattg cggatgccag tattaggcgc agaccgcgaa tggagtgcga
    15781 ttcatcgact gcgggatgtc ggcgttgata ctatgtatgg ggtggcattt ggcgaaaaag
    15841 gtattaatcc gctgagcaga acctcgttta ttattaccga agatctgacc ccaaccatca
    15901 gtctggaaga ttactgtgct gactgggcga ccaacccacc agatgttcgc gtaaagcgta
    15961 tgcttattaa gcgtgtcgcg acgatggtgc gcgatatgca tgctgcgggc attaaccacc
    16021 gtgactgtta tatctgtcat ttcctgctgc acttgccttt ttccggtaag gaagaggagt
    16081 taaaaatttc ggtaattgac ctacaccggg cgcagcttcg cacgcgcgtt ccacgtcgtt
    16141 ggcgcgataa agatcttata gggctttatt tttcttcaat gaatattggc ctgactcagc
    16201 gggatatctg gcggtttatg aaagtgtatt ttgccgcccc gcttaaagac attctcaagc
    16261 aggaacaagg actgctgtcg caagcagaag caaaagccac aaaaatcagg gaaagaacga
    16321 ttcgaaaatc gttgtaattt ccggagagaa atatgagtgc ccactatttt aatccacaag
    16381 agatgatcaa taagacaatc atcttcgatg aaaggccagc ggcgtcagtg gcatcatcat
    16441 tccatgttgc ttatggcatt gataaaaact ttctttttgg ttgtggtgtt tcaatcacgt
    16501 cagttttgtt acataacaac gacgtgagtt ttgttttcca cgtttttatt gatgatatcc
    16561 ctgaagccga tatccagcgt ttagcccaat tggcgaaaag ctatcgtacc tgtatccaga
    16621 tccatctagt aaattgtgaa cggcttaagg cattaccgac gaccaaaaat tggtctattg
    16681 ccatgtattt ccgttttgta attgcagatt actttattga tcaacaagat aagatccttt
    16741 acctggatgc tgatatcgcc tgtcagggaa acttaaagcc gctgataaca atggatcttg
    16801 ccaataacgt tgctgctgtt gttactgaac gcgatgctaa ctggtggtcg ttacggggtc
    16861 aaagtctgca gtgtaatgaa cttgaaaagg gctactttaa ttcaggtgtc ctgttaatta
    16921 atacactagc gtgggcgcag gagtccgttt ctgctaaagc gatgtcgatg cttgctgata
    16981 aagccatcgt ttcccgtttc acctatatgg atcaagatat ccttaatctt atcctgttag
    17041 ggaaagttaa attcattgat gctaaataca atacgcaatt tagtttaaat tatgaattaa
    17101 aaaaatcatt tgtttgtcca attaatgatg aaaccgtatt aattcattat gtcggcccga
    17161 caaaaccctg gcattactgg gccggttatc caagtgcgca accttttatc aaagccaaag
    17221 aagcatcgcc ctggaaaaat gaaccgttaa tgcggccagt taactcaaac tatgctcgtt
    17281 attgcgccaa gcataatttt aaacaaaata aaccaattaa cgggataatg aattatattt
    17341 attattttta tttaaagata ataaaatgaa attgagaaaa gttaaatgaa tgaatttata
    17401 aaagaacggt tttcgtattt agcagataat aaaaaagaaa acgccccaga gctaaatgtt
    17461 tcctacggta tcgataagaa ttttttgtat ggtgctggcg tttcaatttc ttccgttttg
    17521 attaataatt cagatattaa ttttgtcttt catgttttca ctgattatgt ggatgatgat
    17581 tatttaaagt catttaatga aacagcaaaa caatttaata cctcaattat tgtatattta
    17641 attgacccca aatactttgc tgatctgccg acgtcacagt tttggtcata cgcgacatac
    17701 ttcagggtat tgtcttttga atatctgagt gaaagtattt ccacactgct gtatctggat
    17761 gccgatgttg tttgtaaagg aagcctgaaa cctctcacag aaattatatt taaagatgag
    17821 tttgctgcgg tcattcctga caatgatagt actcaggcgg catgtgcaaa acgcctcaac
    17881 attcccgaaa tgaatggacg ttatttcaat gcaggcgtta tctatgtcaa tcttaaaaaa
    17941 tggcatgaag caaatttgac accgtattta ctcaaacttt tacgagggga aactaaatat
    18001 ggctctctta aatatttaga tcaggatgcg ttgaatatcg catttaatat gaataatatc
    18061 taccttgcga aggattttga tactatttat accctgaaaa acgaacttta tgatcgtagt
    18121 catcgaaagt atcagcaaac cattaccgat aaaacagtgt tgattcacta tacagggata
    18181 actaaaccat ggcatagctg ggctggatat ccgtctgcat catactttaa tatcgcgcgt
    18241 gaacaatctc cctggaagaa atatcctctt aaagaggcgc ggactgttgc agaaatgcag
    18301 aaacaatata agcatctgtt tgcccatggt gagtatataa aaggcataac ttcattaatt
    18361 aagtacaagc ttaagaaata aattactctt atgattacaa gtatacgtta tcgcggcttc
    18421 tcattttatt acaaagataa tgataataaa tataaagaaa tctttgatga aatactggcc
    18481 tataatttta aaacagtaaa agtattatgt aatattgatg ataccaaagt atcgttgatt
    18541 gatacgaaat atggtcgcta tgtttttaaa gtctttgctc cgaaaacgaa aaggaatgaa
    18601 cgttttttaa aatcttttgt aaaaggtgac tactatcaga atttgatcgt tgaaaccgat
    18661 cgtgtcagaa gtgcaggtct tacttttcct aatgattttt attttctggc tgagcgtaaa
    18721 attttcaact atgccagtgt ttttatcatg cttatcgagt atgtcgaggg cgtagagctc
    18781 aatgatatgc cgattattcc agaaaatgtt aaagcagaaa ttaaggcttc gatggaaaaa
    18841 ttgcatgcct taaatatgct atcgggcgac ccacaccggg gtaactttat cgttagtaaa
    18901 gatggtgtca ggataattga cctatccggt aaaagctgta cggcagagcg taaagctcgt
    18961 gatcgtctgg ccatggaacg tcacctgggt attgccaacg aaataaaaga ttatggctac
    19021 tattcagtaa tctacagaac caaactacgc aaatttatta aaaaattaaa aggcaaagcg
    19081 taaaccacac agtcaaaacg gaaccaacat ggatttatta gcagagagta ttactgaagt
    19141 cgctgtctct ggggaaattg ctaacaccga tcgtgtgtta aatatcgctt acggtattga
    19201 ccgtaacttt ttatttggtg cggcagtatc tatgcaatca gttgttatgc ataacccgga
    19261 ccttgcggtt aagtttcatc tctttaccga ctacattgat gaagattatc tacaacgtgt
    19321 taatgctttt accagcaaaa atgctaacgt tgaagtaata atttataaag tctccaatgc
    19381 ctttattgat atcttcccca gcctgaaaca gtggtcttat gcaacattct tccgtttagt
    19441 tgcgttccag tatttgagtg aaactattga aaatctgtta tatatcgatg ctgatgtcat
    19501 ctgtaaaggc tcattagctg gattgcttga tattaatttt gatgaggata agttcgcagc
    19561 tgttattaaa gatgtgcctt ttatgcagga aaaaccagcg aagcgtctgg ctatagaggg
    19621 acttccaggg aattatttca acgccggtgt agtatatctg cagcttgaag catgggcgaa
    19681 aaatgatttt atgaataaag ccattgctat gctggcaagt gacccgcagc acacgaagta
    19741 taaatgcctt gatcaggata ttttaaatat tctgttcttt ggtcattgta attttattag
    19801 cggcgattat gattgctttt atggcattga ctatgagtta aaaaataaaa gcgatgaaga
    19861 ttataaaaag accattaccg atgatactaa gctgattcat tatgttggcg taacgaagcc
    19921 ctggaacgac tggacgaatt atccctgcca gaagtatttt aatgaggctt atcaggcttc
    19981 ttgctggaat gatgtggcgt ttattccagc cacgaatgaa aagcagtatc aagtgaaata
    20041 tcaacatgca aagaaaaatg gtgatacgtt taacgctttt atttacttca ttaaatttaa
    20101 attaaataag tataaaagaa aactatttgg ataataataa aaatcatgga tgtctgagtt
    20161 acaataataa ttaaggatga gtactatcat attttattag ttcaggcaag agaacacgat
    20221 gagcaatgat taccctttag tatctataat aataccgacg tataattcat ctgattacat
    20281 tactgaaact ctaacgaaat tagaaaaaca aacttaccca aattttgaaa ttgttattgt
    20341 taatgatggt tctaaagata acacatcaaa cgttttgaga gagtatgggt taacccactc
    20401 tcgattaatt attgtcaata aagaaaatgg cggtgtttcg tctgccagga atacaggtat
    20461 ccgcaaggcg caaggacagt ttatatgttt tatggatgat gatgatgaga tagatcctaa
    20521 ctatctgctg aagatgtata ccagacaaca tgagacggga ggggatgcga tttattgtgg
    20581 gctttatggc cattatataa aagatggtgt tacttactca cctataaata cagagtttaa
    20641 tgaaggatct ttacttttcg acttttttta taaaaaggtt agattccata tagggtgctt
    20701 gtttataaga aaacaacttc tggaagagaa taatcttttt tttgatgaag atttacgact
    20761 aggagaagat cttgatttta tctatcgact gctaattaca tgcgatatgt atgcggttcc
    20821 atattatatg tataagcata actatagaga aaattcctta atgaactcat gtagaaccat
    20881 cactcattat cgacatgagt catttgcgca cgaaagaatc tactcttctg tgatgcagtt
    20941 atacaaaggt aaccggaaag aagaaattca tacattattg agtcaaaata gagcttatca
    21001 taaaactcgt tatttatgga atgttctact taatggtgat tttaaactat tgaatcaatt
    21061 agttgaaagc aatgaaaaag aattaagaga ttgtaatctt cctggcaaga gagataagag
    21121 acgagcaaaa atattagcat caaaaaatta tattctctgg aggatggtaa gactggtaaa
    21181 tagaaaaaag aataaacgtt agatcttgca ttaaataacc catcctcgta gcataggttg
    21241 aaattatatg tcgttttgtt ggaatgaaat taattctggt ataaagtctt taattcttat
    21301 attatgtatt ttttctttaa tgactttgtc tttatgggat gatgttgcaa caaagtttct
    21361 tcatgcagct ggaattatat ctgcattgta ttttcttgcg acaccaaaaa aaacaataac
    21421 taataatcct actttgttaa ttttcatctc attatgtctt ttgggtatcg taaatatcat
    21481 ctggtattca cattataaag tttcaggctc tgtttatacc aatgcatatc gtggcccaat
    21541 ggaaactgga aaaattgcct tgtgtagcgc ctttattttc ttagttcttt ttgctaaaaa
    21601 tgagatgaga acaaaaataa aatttgggaa actaattctg ttcgcatccc tggcaacgca
    21661 gttacttttt tttgcgcatg ccatgtggca acatttctat ttaaacgtcg accgtgttgc
    21721 attatcagct tcccacgcta caacagcagg ctacatcatc ctttttcctt ctttactggc
    21781 atcaattctc attttaaaat ccgactttag acataaaaca acattatata caattaactt
    21841 catgcttagc ttatgtgctg tcatagtaac tgagacgcgt gcagccatat tagtgtttcc
    21901 attctttgcg ttaatattaa tcgtaatgga tagttatatt aataagcgaa ttaattataa
    21961 gttatattgt tttattgcga ttgcattatt agcaggtgta ttttctttta aagatacatt
    22021 gcttatgaga atgaatgact taaataacga tttagttaat tattcgcatg ataacaccag
    22081 aacttcagtc ggtgcccgtc tggcaatgta tgaagttggc ttaaaaacat attctccaat
    22141 aggacaatca ctggaaaaac gtgcagaaaa aatacatgag ctagaagaaa aagagcctag
    22201 attgagtggc gctttacctt ttgtagattc tcatttgcat aacgatctca tagatacgtt
    22261 atcaacgcgt ggtattcctg gagttgtatt aacaatttta gcattttcag caatactcat
    22321 atatgcctta agaactgcta aagaacctta tattttaatc ttgctttttt cactactggt
    22381 agtaggccta agtgatgtaa tactcttttc taaaccggtt ccgactgctg tgtttgtcac
    22441 cataatattg ctttgtgctt attttaaagc acaatcagac caatatttat tagataagta
    22501 atgaatatat gaaataaaat cagcaaggag gctgatttta tttcaaaatt atctattaag
    22561 gggttgtaat ttaaatcata gcagcgtttt cttcaatgaa tcgcttaaca gcatttgctg
    22621 tcaattgaga caactcattc cccggagccc tacaaaccat ttgattcttc ccatacccac
    22681 caattaatcc cggatcggtt ggtccataaa ccgtgatatt gggtctatcc agtgccgccg
    22741 ttaaatggct taaccccgta tccaccgaca ctacaaattt agccccggcc agcacgcggg
    22801 caacgccttc cagactcatc ttcggcaata cttcaacata atcaaatcct tccgccagtc
    22861 gtttcgcccg ttcttcctca tgcggcgcgc cccacggaag tttaatccgt attcctgaat
    22921 cagccagtaa accaatcaat tctcgccagt gttcttccgg ccagtgttta tcatcacggg
    22981 tcgtcgcatg aagaaatacg gcatattcgc cagcatctgt aggcaggttc gtcagaaaat
    23041 gctgtgcgat agcataatcg ccctgggttt gcggtttgct atagcccaaa cttttggcaa
    23101 acagttcgcg ggtgcgttct acggcgtgct gctgttttgc aatatgatgc ttacaattgt
    23161 aaaacaggct ggctaaaggt tcgcgagcgg tttgccagtc caatccatgc tttacgccat
    23221 gcgccagacg tgtcaccagt gccgcgcttt ttaccagccc ctgagcgtcg ataactgcgt
    23281 catagttctc tgcttgtagc gcttcacgaa acgctttgcg ttcagctttt atgggggccg
    23341 agaaccaggc tttacgccag cgacgtattg ccacaggaat aactcgctca acggcagcgt
    23401 gccaggaagg aatctgtgcg aacccttctt ccaccaccca gtcaaactta atccctggga
    23461 ttgcctgctg ggcatcagtg agtgcgggca acgtatggag aacatcgccc atcgacgatg
    23521 ttttaacgat caaaacccgc atccgtcagg cttcctcttg taacaatagc gtgttgagtt
    23581 cttccagtac gcgctgggga gtaatgtcga tcaagctctg gtgataaccc tccgcagcgt
    23641 cacctttacg cactttgtga tagccggtaa tcagacggat cacgcgcgct ttatgggata
    23701 gcggcggtgt gaagtccggg ctacttggac catacagggc aaccagcgga cgattgagcg
    23761 ccgccgcaac gtgcatcagg ccagaatcgt tagtgacaat ggctttacag gctgcaatca
    23821 ggataaccgc ttgatcaagc tgtgtttccc ccgccaggtt ccgacaccat gcctgctgct
    23881 cggtattcaa agcggcaaga atctcattgc ccgcttcatg atctttcgca gagccaaaca
    23941 gaaccacctg ataaccttca tcaatcagct gctttgccag ttccgcatag tggtagtgtg
    24001 gccagcgttt tgccggacca aactccgcac ccgggcaaaa gccaatcatc ggacgttctg
    24061 atgaaagcga aaattgatta caggtatatg atttttcacc ttcgctcacc tgcaactgcg
    24121 gccataacaa tggctgcggc agatcttgtg cggtacgcat aatgcctttg tcataggcca
    24181 gcgcgacata gcgttccacc attagcggcc aggcttcttt atcgagcacg cgtacatcgt
    24241 tgagtaaacc gtagcgcatc tcgccgcgcc agccggtgcg atgaggaata cccgcgaaga
    24301 aaggcactaa tgcagatttg aaggagttgg gtaagacgta ggcgcggtcg tagcgctttt
    24361 cacgcaggct atgacccagt ttgcggcgtt cgccgatttc cagcgctccg tgaccgagag
    24421 gcatagggat agcttcgtta acttccggca tccgcgataa taatggacgg caccatgccg
    24481 gtgccatcac atcgattatc gcctggggat agcgcgcctg gagcgtgcga tagagacttt
    24541 gcgacatcat catgtcgcca acccaagacg ggccgatcac cagtattttc atgcagagct
    24601 cttatgcgtc gcgattcagc caggccatgt attccgttac accttcagca acggttttga
    24661 acggtttgtc gtaacccgcc gcgcgcagat ttgtcagatc tgcctgagtg aacgcctggt
    24721 agcggccttt cagcttatcc gggaacggaa tgtattcgat ctggcctttc ttgtgataag
    24781 ccagagtagc atctgctacg gcctggaagg attccgcacg accagtaccg aggttgaaga
    24841 taccggaaac gccattttcc aggaaccaca ggtttacatc tgccacgtcg cccacataga
    24901 cgaagtcacg tttgaagttc tcgctacctt caaacagctt cggtgattca ccgttgttaa
    24961 gctgagtgtt gagatggaaa gcgacgctcg ccatgctgcc tttatggcct tcacgcggtc
    25021 cataaacgtt gaaatagcgg aagccaacaa tctgcgagtt cgcttcaggc aggatttggc
    25081 gaacatattc atcaaacagg aattttgagt aaccgtagac gttcaacggt ttttcgtact
    25141 cgcgggattc aataaagtcg gaggtgcgtc cgccgtaggt ggctgcggaa gaagcgtaca
    25201 ggaacgggat ttcgcgctcc aggcagtagt gcagcagctc cttggagtat tgatagttgt
    25261 tatccatcat atacttgccg tcccactcgg tggtggaaga gcatgcgcct tcgtggaaaa
    25321 tcgcttcgac atcgccgaac tcttcgccag ccataatctg gatcaggaag tcttccttat
    25381 ccatatagtc cgcgatatcc agatccacca ggttcacaaa cttggtgccg tctttcaggt
    25441 tgtccaccac cagaatatcg gtgatgcctt tatcattcag ggccttaacg atgttgctgc
    25501 cgataaagcc cgcgccgccg gtaacgatga tcataactgt aaccttcgaa ttatggagtc
    25561 agagacaatc tcagacacga atacttctat catatcacta tacggcgttg gcttcagcca
    25621 ttcgccaaaa gtgagattga tggacgttat ttatcctgaa ttttgcagaa gtgttaacgc
    25681 gtcatctcgt cgcgacgtat aagtttgggt aatatgtgct ggaatttgcc ctgtctggag
    25741 aatcgcaatg cgtggagaat tttatcagca gttaaccaac gatctggaaa ccgcacgggc
    25801 ggaagggttg tttaaagaag agcgcattat tacgtctgcg cagcaagcag atatcactgt
    25861 ggctgatgga agccacgtca ttaacttttg tgccaacaac tatctcgggc tggcgaatca
    25921 tccggatctg attgcggcag caaaggcggg aatggattct cacggtttcg gcatggcttc
    25981 ggtgcgtttt atttgcggca ctcaggacag ccataaagag cttgaacaaa aactggcggc
    26041 attcctgggg atggaagatg cgattctcta ctcttcctgc tttgatgcta acggtggact
    26101 gtttgaaacg ctgctgggtg cggaagacgc cattatctcc gacgcactga accacgcgtc
    26161 tattattgat ggtgtgcgtc tgtgcaaagc taaacgctat cgctatgcca acaacgatat
    26221 gcaggagctg gaagcacgtc tgaaagaagc gcgtgaagcc ggtgcgcgtc atgtgctgat
    26281 tgccaccgat ggtgtgttct caatggacgg cgtgattgcc aacctgaaag gcgtttgcga
    26341 tctggcagat aaatacgatg ccttggtgat ggtggatgac tcccacgcgg tcggttttgt
    26401 cggtgaaaat ggtcgtggtt cccatgaata ctgcgatgtg atgggccggg tcgatattat
    26461 caccggtacg cttggtaaag cgctgggcgg ggcttctggt ggttataccg cggcgcgcaa
    26521 agaggtggtt gagtggctgc gccagcgttc tcgtccgtac ctgttctcca actcgctggc
    26581 accggccatt gttgccgcgt ccatcaaagt actggagatg gtcgaagcgg gcagcgaact
    26641 gcgtgaccgt ctgtgggcga acgcgcgtca gttccgtgag caaatgtcgg cggcgggctt
    26701 taccctggcg ggagccgatc acgccattat tccggtcatg cttggtgatg cggtagtggc
    26761 gcagaaattt gcccgagagc tgcaaaaaga gggcatttac gtcaccggtt tcttctatcc
    26821 ggtcgttccg aaaggtcagg cgcgtattcg tacccagatg tctgcggcgc atacccctga
    26881 gcaaattacg cgtgcagtag aagcattcac gcgtattggt aaacaactgg gcgttatcgc
    26941 ctgaggatgt gagatgaaag cgttatccaa actgaaagcg gaagagggca tctggatgac
    27001 cgacgtccct gtaccggaac tcgggcataa cgatctgctg attaaaatcc gtaaaacagc
    27061 catctgcggg actgacgttc acatctataa ctgggatgag tggtcgcaaa aaaccatccc
    27121 ggtgccgatg gtcgtaggcc atgaatatgt cggtgaagtg gtaggtattg gtcaggaagt
    27181 gaaaggcttc aaaatcggcg atcgcgtttc tggcgaaggc catatcacct gcggtcactg
    27241 tcgcaactgt cgtggcggtc gtacccatct gtgccgcaac acgatcggcg tcggcgttaa
    27301 ccgtccgggc tgcttcgccg aatatctggt gatcccggcg ttcaacgcct tcaaaatccc
    27361 agacaatatc tccgacgacc tggcttccat ttttgatccc ttcggtaacg ccgtgcatac
    27421 cgcgctgtcg ttcgatctgg ttggcgagga tgtgctggtc tctggtgcag gtccgatagg
    27481 tattatggct gcggcggtgg cgaaacacgt tggtgcacgc aatgtggtga tcactgatgt
    27541 taacgaatac cgccttgagc tggcgcgcaa aatgggtatc acccgtgcgg ttaacgtcgc
    27601 gaaagaaaac cttaatgatg tgatggctga actgggcatg accgaaggct ttgatgtcgg
    27661 tctggaaatg tccggtgcgc cgccagcgtt tcgtaccatg cttgacacca tgaaccacgg
    27721 cggccgtatt gcgatgctgg gtattccgcc gtctgatatg tctatcgact ggaccaaagt
    27781 gatctttaaa ggcttgttca ttaaaggtat ttacggtcgt gagatgtttg aaacctggta
    27841 caagatggcg gcgctgattc agtctggcct cgatctctcg ccgatcatta cccatcgttt
    27901 ctctatcgat gatttccaga agggctttga cgctatgcgt tcgggccagt ccgggaaagt
    27961 aattctgagc tgggattaac acgaacaagg gctggtattc cagccctttt atctggggat
    28021 aatctgttga atatgtaaaa tcctgtcagt gtaataaaga gttcgtaatt gcgctgatct
    28081 cttatatagc tgttctcatt atctctctac cctgaagtga ctctctcacc tgtaaaaata
    28141 atatctcaca ggcttaatag tttcttaata caaagcctgt aaaacgtcag gataacttca
    28201 gaggtcgtcg gtaatttatg atgaacagca ccaataaact tagtgttatt attccgttat
    28261 ataatgcggg cgatgatttc cgcacttgta tggaatcttt aattacgcaa acctagactg
    28321 ctctggaaat cattattatt aacgatggtt caacggataa ttctgttgaa atagcaaagc
    28381 attacgcaga aaactatccg cacgttcgtt tgttgcatca ggcgaatgct ggcgcatcgg
    28441 tagcgcgtaa tcgtgggatc gaggtggcga cgggcaaata tgtcgctttt gtcgatgctg
    28501 acgatgaggt ctatcccacc atgtacgaaa cgctgatgac catggcgtta gaggacgacc
    28561 tcgacgtggc gcagtgcaac gctgactggt cttttcgtga aacgggagaa acctggcaat
    28621 ccatccccag cgatcgcctt cgctcaaccg gcgtattaac cggcccggac tggctgcgga
    28681 tggggctttc ttcgcgccgt tggactcacg ttgtctggat gggggtttat cgccgtgatg
    28741 ttattgttaa aaataacatt aaatttattg ccggattaca tcatcaggat attgtctgga
    28801 cgacagaatt catgtttaac gcgctgcgtg cgcgatatac cgagcaatca ttatataaat
    28861 attatctgca taatacgtca gtgagtcggt tgcatagaca agggaataaa aaccttaatt
    28921 atcaacgtca ctatattaag attacccgcc tgctggagaa attaaatcga aattatgccg
    28981 acaaaattac gatttatccg gaatttcatc agcaaataac ttacgaagca ttgcgtgttt
    29041 gccatgcggt gcgcaaagag ccggatattc ttacccgcca acggatgatt gccgagatat
    29101 ttacttccgg tatgtataag cgcctgatta ccaatgtgcg cagcgtgaag gtcggttacc
    29161 aggcgttact gtggtctttc cgcttatggc aatggcgcga caaaacgcgg tcgcaccatc
    29221 gcattacgcg tagcgccttt aatttgcgct agcgttaaac ttcgccgctt cgggctgttt
    29281 gccccagcct tgccactgat gctggaagta aacgatcagc gtgctctggc tgatgctttc
    29341 gcttaatact tcaaagaagc gattagcata aacaggttct atcggtttct tcggtttgca
    29401 cagcttcacg ccacggaacg gattacgcgg tgcatcaggc acagcgtttt tcggcggtgt
    29461 agaagtatca acctgcggtt cattcagcaa gctgctggct ttcaccagcg taatgtctgg
    29521 cggcaggtta taaaccatct gttgtagcac gcgtaccgtt aaagggtgag gatgcccaat
    29581 ggcaatggtt gaaccgttgc gacgcgccag atcaattgcg cgattaaatt gcacacggat
    29641 gtccgcttcg ttctgcgaat cgtcgaggaa caccttccgt ttaataacct tcacgccagt
    29701 accttgcgcg gcgcgcatcg cctgggtatt accgatggtt acgctgtcga ggaagtaaag
    29761 attgtaacgc tccagtgcct gcatcacttt ctgcatacca aacaggttag aggtcatctt
    29821 gctacccatg tggttgttga tccccacggc atagggcacg ttattgaccg cactacgaat
    29881 aatgcgctca atttcgtcgc tgctcatctc cgggcgcagc gtatttttct ccagcggctg
    29941 tttactcagt ggtgccatcg ggagatgaat caacacttcg tgcccgctgt tatgcgcttt
    30001 ggtcgccatc tctctggcgt gcggtgaatc gggtaataca gcgacggaga tagcggaggg
    30061 catcgccagc acctggtttt cgttgtgcgg gcgataccca aaatcatcaa tgacgatggc
    30121 aagtttgcca gcaagtacgg gggaggagag cgccaacaga gcggcaaatg caagaacgtt
    30181 acgacgaaat ggaaacaaaa cttatcttcc caaccacggc tgtggattga ccgcctgacc
    30241 ctggcggcga atttcgaaat agagtgaagg ccgaccctga ccgccactgc tgcccaccag
    30301 tgcaattggc tggcccgcgc gaacctgcga accaacgctc accagtgcgc tctgattata
    30361 gccgtaaaga ctcatgtcgc ctttaccgtg ctcaaccacc accaccagac cgtaaccttg
    30421 cagccagtca gccagaatca ctcgaccgtc ggcaattgct ttaacttcag taccttcaga
    30481 ggcaccgata accatccctt tccagcgtaa ttcaccctgt agctgctcgc catagcgatg
    30541 cagcgtcggc ccgcgaaccg gccagaatgc ctgaccacgc ggcgcaccca gaccaccagt
    30601 tcgggacatc agcgattttt cgctttcggt cggtttgtag gtggtgcctt tgcgcgtcgc
    30661 ttctttctgg cggtcgcgaa ccgcctgggc ctcacgcgct tctcgttcag cacgcgcttt
    30721 cgccgcggct tccgcacggg caatgctgtt acgcagacgg gattcgttgg cacgcagttc
    30781 acttagctgc tgctgacctt gctggatgga agattccagc cccgccagcg tctttttacg
    30841 ctcgttcaga gcctgagtca gtttcgcctg ttgggcgcgc tgttcatata acagcgtttg
    30901 ttgctcgctt tgtttctctt ccagttcagc acgctgcatg gcgacttctt cacgcgtttg
    30961 cttcaactgg gcaatggttt cttgtcgcgc ctggttgaga tagccgaaat aagcctgtaa
    31021 acgctgtcca cactggcttt cttcaccgct gagaattagc tgaataccgg tatgctcgcc
    31081 ctgacggaat gcggcatcca gttgtgcggc gaggctgcgc tcctgggcgg ctttttgctg
    31141 ctccagtttg gcaatcgacg cgttcatctc atcaatctgt ttattgagtt gattgagcgt
    31201 gttttgcgtt tcgcgcagct tacgggtggc ttcagagatc gcttcttcct gctttttcaa
    31261 ttgtgcgagc aggcttgcgc gttgttgttg cttttggcgt accgcgcgct cttttgcggc
    31321 gatatcggcc tgaatagatt tgagttggtc acgctcatcc gcgtgggcgg aaaaggcgca
    31381 caacaatacg ccagcgctaa gaacgctggc gtagatgatg ggcctgattg caaacctgcg
    31441 cggtttcacg gcccgtgtca tggtattaat cgcctttccc ctcatgggga gggattattc
    31501 cacgatgaac agcggcttac cagtcatctc ttgcgggatt tccataccca tcagcgacaa
    31561 catggtcggc gcgatgtcag aaagtttgcc gcctgcaacc gctttcacgt tcttatcacc
    31621 aacgtaaatc agcggaactg gcaggttggt gtgtgccgtg tgtgcctgac ccgttgccgg
    31681 atcgcgcatc tgttcagcgt taccgtggtc agcggtgatc agcagttgtc cacccacgga
    31741 ctcaaccgct ttcgcgactt cttccacgca gtgatccagt gcttcaaccg ctttaaccgc
    31801 cgcttccatc accccggtgt gacctaccat gtcgccgttc ggatagttac agataatggt
    31861 gtcgtatttg ccgctcttga tggccgcaac cagtttttcg gtcagctctg cggagctcat
    31921 ttccggttgc agatcgtagg tagccacttt cggtgagttg atcagaatgc gatcttcgcc
    31981 tttgaatgac tcttctacgc caccgttgaa gaagaaagta acgtgggcat atttttcggt
    32041 ttcggaaata cgcaactgag ttttgtcgtt tttcgccatc cactcgccga aggtgttaac
    32101 cagggaagcg ggtgggtaag caaccgcagt tttgatgtca gcggcgtatt cggtcagcat
    32161 cacgaaatcg acgttaacca ctttcttacg cgcgaagccg tcgaaatctg cgtttacgaa
    32221 tgcacgagtg atttcgcgcg cgcggtcagc acggaagttc atgaaaatca gcgcatcgcc
    32281 gtcttccatt gccgcatctg gctgaccttc tgcacgaata acggtcgctt tcacgaactc
    32341 gtcgttttcg tcgcgagcat aagctgcctg caaaccagca acggcagtat cggcctggaa
    32401 ctcaccctgc gccagagtca gcaggtcata ggctttttca acgcgatccc aacggttatc
    32461 gcggtccatc gcgtagtaac gaccaatgat ggacgctacg cggcctttac ccagcgcagc
    32521 aaatttctct tcaaattttt tcagcgagga ttcagcactg cgcggcgggg tgtcgcgacc
    32581 gtcaaggaat gcgtgcaggt agattttttc tgcgccgcgt tcagctgcca gttctaccat
    32641 cgccatgatg tgatcttcgt ggctgtgtac gccgcctgcg gagagcagac ccataatgtg
    32701 tactgcttta cctgcgtttt tcgctttatc taccgcgcca gtcagcactg gattagcaaa
    32761 gaaagcgcga tctttgattt caacgtccag acgagtcagg tcctgataca cgatgcggcc
    32821 ggcacccagg ttaacgtggc ctacttcgga gttacccatc tgacggtcag gcagaccgac
    32881 ttccagaccg gaagcgtcga tcagggtatg cggacgattg gcccacagtg catccattac
    32941 cggggtttta gcactaaaaa tggcgttatc ctgctgttct tcacgatagc catagccatc
    33001 cagaatcacc agtaccatag gttttttaga aaccgacatt gcgacaacct catactcaag
    33061 agtcaaaatt tgcgtaattt tactacagct gaatcgataa aatagcctct gaagatcaaa
    33121 atatacgacc gctcgcggca agccgatgcc agatttgcgc ttttttttcg tcgtgccccg
    33181 caaaattgca ttccagttaa cgcgctggct gtatttgccg caccgcgcag gtatactcct
    33241 ttcctggttt ttttaatcac tacgtcggga gttgttaccc cccatgcaag aaattatgca
    33301 atttgttggc cgtcatccca tactgagtat cgcttggatc gcgttactgg tggcggttct
    33361 tgtgactacg tttaagagcc tgacctcgaa agtgaaggtg attactcgtg gtgaagctac
    33421 gcgtctgatc aacaaagaag acgctgtggt tgtggattta cgtcagcgtg atgacttccg
    33481 taaaggccat atcgcaggtt ctatcaatct gttgccgagc gaaatcaaag ccaacaatgt
    33541 tggtgagctt gagaagcaca aagacaaacc ggttatcgtg gtagacggtt ctggcatgca
    33601 gtgccaggag cctgcaaacg cgctgacgaa agctggtttt gcgcaagtat tcgtgctgaa
    33661 agagggcgtt gctggctggg ctggcgaaaa cttgcctctg gtgcgcggca aataatttac
    33721 ctgtcgtctt tcatgatgca ggccgttagc tgcaattagg cccccagcga cttattgtta
    33781 taagcttctg gggacactcc attttgcagc cgccctgcaa catgaaattt cagggtaaaa
    33841 aaacaggagc taaattatgg ccaatgttga aatctatacc aaagaaacct gcccgtattg
    33901 ccatcgtgca aaagcactgc tgagcagcaa gggcgtgagt tttcaggagt tgccgatcga
    33961 tggcaatgcc gccaagcgtg aagagatgat caaacgcagc ggtcgcacca cggttcccca
    34021 gatttttatt gacgcacagc acattggcgg ctgtgatgac ttgtatgcat tggatgcacg
    34081 tggtggactg gatcccctgc tgaaataacg tgtgaacgtt ggcattacat tgcgcagtat
    34141 ttaaggacaa cacttaaggg ttttctacac atgtcagaac aaaacaacac tgaaatgact
    34201 ttccagatcc aacgtattta taccaaggat atctctttcg aagcgccgaa cgcgccgcac
    34261 gttttccaga aagattggca accagaagtt aaacttgatc tggatacggc atcttcccaa
    34321 ctggcagatg acgtatacga agtggtactg cgtgttaccg taacggcctc cctgggcgaa
    34381 gaaaccgcgt tcctgtgtga agttcagcag ggcggtattt tctccatcgc gggtatcgaa
    34441 ggcacccaga tggcacattg cctgggcgca tactgcccga acattctgtt cccgtatgct
    34501 cgtgagtgca tcaccagcat ggtatcccgc ggtacattcc cgcaactgaa ccttgcgccg
    34561 gttaacttcg atgcgctgtt catgaactat ttgcagcagc aggctggcga aggtactgaa
    34621 gaacatcagg atgcctgatg aaccaacgta atgcttcaat gactgtgatc ggtgccggct
    34681 cgtacggcac cgctcttgcc attaccctgg caagaaatgg ccacgaggtt gtcctctggg
    34741 gccatgaccc tgaacatatc gcaacgcttg aacgcgaccg ctgtaacgcc gcgtttctcc
    34801 ccgatgtgcc ttttcccgat acgctccatc ttgaaagcga tctcgccact gcgctggcag
    34861 ccagccgtaa tattctcgtc gtcgtaccca gccatgtctt tggtgaagtg ctgcgccaga
    34921 ttaaaccgct gatgcgtcct gatgcgcgtc tggtgtgggc gaccaaaggg ctggaagcgg
    34981 aaaccggacg tctgttacag gacgtggcgc gtgaggcctt aggcgatcaa attccgctgg
    35041 cggttatctc tggcccaacg tttgcgaaag aactggcggc aggtttaccg acggctattt
    35101 cgttggcctc taccgaccag acctttgccg atgatctcca acaattgctg cactgtggca
    35161 aaagtttccg cgtttacagc aacccggatt tcatcggtgt gcagcttggc ggtgcggtga
    35221 aaaacgtcat tgccattggc gcggggatgt ccgacggtat cggttttggt gcgaatgcgc
    35281 gtacggcact gatcacccgt gggctggctg aaatgtcgcg tcttggcgcg gcgctgggtg
    35341 ccgagcctgc cacctttatg ggcatggcgg ggctgggcga tcttgtgctt acctgtaccg
    35401 acaaccagtc gcgtaaccgc cgttttggca tgatgctcgg tcagggcatg gatgtacaaa
    35461 gcgcgcagga gaagattggt caggtggtgg aaggctaccg caatacgaaa gaagtccgcg
    35521 aactggcgca tcgcttcggc gtagaaatgc caataaccga ggaaatttat caagtattat
    35581 attgcggaaa aaacgcgcgc gaggcagcat tgacgttatt aggtcgtgca cgcaaggacg
    35641 agcgcagcag ccactaaccc cagggaacct ttgttaccgc tatgacccgg cccgcgcaga
    35701 acgggccggt cattatctca tcgtgtggag taagtaatgt cgtgtgaaga actggaaatt
    35761 gtctggaaca atattaaagc cgaagccaga acgctggcgg actgtgagcc aatgctggcc
    35821 agtttttacc acgcgacgct actcaagcac gaaaaccttg gtagtgcact gagctacatg
    35881 ctggcgaaca agctgtcatc gccaattatg cctgctattg ctatccgtga agtggtggaa
    35941 gaagcctacg ccgctgaccc ggaaatgatc gcctctgcgg cctgtgatat tcaggcggtg
    36001 cgtacccgcg acccggcagt cgataaatac tcaaccccgt tgttatacct gaagggtttt
    36061 catgccttgc aggcctatcg catcggtcac tggttgtgga atcaggggcg tcgcgcattg
    36121 gcaatctttc tgcaaaacca ggtttctgtg acgttccagg tcgatattca cccggcagca
    36181 aaaattggcc gtggcattat gctcgaccac gccacgggca tcgttgtggg tgaaacggcg
    36241 gtaattgaaa acgacgtatc gattctgcaa tctgtgacgc ttggcggtac gggtaaatct
    36301 ggtggtgacc gtcacccgaa aattcgtgaa ggtgtgatga ttggcgcggg cgcgaaaatc
    36361 ctcggcaata ttgaagttgg tcgcggcgcg aagattggcg caggttccgt ggtgctgcaa
    36421 ccggtgccgc cgcatacaac cgccgctggc gttccggctc gtattgtcgg taaaccagac
    36481 agcgataagc catcaatgga tatggaccag catttcaacg gtattaacca tacatttgag
    36541 tatggggatg ggatctaatg tcctgtgatc gtgccggatg cgatgtaatc atctatccgg
    36601 catacagtaa ctaatctctc aacaacgctc ccggatatcc cagctgccgc caggcttcat
    36661 acaccactac cgacaccgca ttggacagat tcatgctgcg gctgtccggc accatcggaa
    36721 tgcgaatttt ttgttcagcg ggcagggcat caagaatgct cgctggcagg ccgcgtgttt
    36781 ccgggccgaa catcagataa tcgccatcct gatagcttac ggcgctgtga gcaggcgtac
    36841 ctttcgtggt gagggcaaac aggcgctggg gattttctgc ttcgaggaac gcgcgatagt
    36901 catgatgacg cgtaacggcg gtaaactcgt gatagtccag ccccgcgcgg cgcaggcgct
    36961 tatcgtccca ggcaaatccc atcggttcga tgatatgcag acgaaagccg gtattagcgc
    37021 aaagacggat gatgttgcca gtatttggcg gaatttctgg ttcgtaaagt acgatgttta
    37081 gcatactgcc cccttagtgc gggggcagga tagcagaaaa ttggtgaacc gtgcggcctt
    37141 ttcccctcac cccggccctc cccctgtctc ttgatcagga gatctgatca agagacagct
    37201 ccctgaggga gagggt
//
LOCUS       DAJTSL010000020        36531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTSL010000020 DAJTSL010000000
VERSION     DAJTSL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.020"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..896
                     /locus_tag="OO433_000890"
     CDS             <1..896
                     /locus_tag="OO433_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6049460.1"
                     /translation="DIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEEQ
                     IGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNTY
                     PTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELENL
                     TISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGINP
                     TIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIR
                     NHRYTATMLQHIAEDRDGTNH"
     gene            complement(893..2370)
                     /gene="scpC"
                     /locus_tag="OO433_000891"
                     /pseudo
     CDS             complement(893..2370)
                     /gene="scpC"
                     /locus_tag="OO433_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(2394..3179)
                     /gene="scpB"
                     /locus_tag="OO433_000892"
     CDS             complement(2394..3179)
                     /gene="scpB"
                     /locus_tag="OO433_000892"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6049461.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(3190..4185)
                     /gene="meaB"
                     /locus_tag="OO433_000893"
     CDS             complement(3190..4185)
                     /gene="meaB"
                     /locus_tag="OO433_000893"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6049462.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPSAGKRTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKIRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(4178..6322)
                     /gene="scpA"
                     /locus_tag="OO433_000894"
                     /pseudo
     CDS             complement(4178..6322)
                     /gene="scpA"
                     /locus_tag="OO433_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(6526..7419)
                     /gene="argP"
                     /locus_tag="OO433_000895"
     CDS             complement(6526..7419)
                     /gene="argP"
                     /locus_tag="OO433_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6049463.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7802..8461
                     /gene="rpiA"
                     /locus_tag="OO433_000896"
     CDS             7802..8461
                     /gene="rpiA"
                     /locus_tag="OO433_000896"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6049464.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8716..9948
                     /gene="serA"
                     /locus_tag="OO433_000897"
     CDS             8716..9948
                     /gene="serA"
                     /locus_tag="OO433_000897"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6049465.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            10177..10236
                     /gene="ibsC"
                     /locus_tag="OO433_000898"
     CDS             10177..10236
                     /gene="ibsC"
                     /locus_tag="OO433_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6049466.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10569..10628
                     /locus_tag="OO433_000899"
     CDS             10569..10628
                     /locus_tag="OO433_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6049467.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10729..11331)
                     /locus_tag="OO433_000900"
     CDS             complement(10729..11331)
                     /locus_tag="OO433_000900"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6049468.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11352..11535)
                     /gene="ssrS"
                     /locus_tag="OO433_000901"
     ncRNA           complement(11352..11535)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO433_000901"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11577..11906)
                     /gene="zapA"
                     /locus_tag="OO433_000902"
     CDS             complement(11577..11906)
                     /gene="zapA"
                     /locus_tag="OO433_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6049469.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            12074..12652
                     /gene="ygfB"
                     /locus_tag="OO433_000903"
     CDS             12074..12652
                     /gene="ygfB"
                     /locus_tag="OO433_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6049470.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12678..14003
                     /gene="pepP"
                     /locus_tag="OO433_000904"
     CDS             12678..14003
                     /gene="pepP"
                     /locus_tag="OO433_000904"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6049471.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            14000..15178
                     /gene="ubiH"
                     /locus_tag="OO433_000905"
                     /gene_synonym="visB"
     CDS             14000..15178
                     /gene="ubiH"
                     /locus_tag="OO433_000905"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6049472.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            15201..16403
                     /gene="ubiI"
                     /locus_tag="OO433_000906"
                     /gene_synonym="visC"
     CDS             15201..16403
                     /gene="ubiI"
                     /locus_tag="OO433_000906"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6049473.1"
                     /translation="MQCVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADISLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16477..16560)
                     /locus_tag="OO433_000907"
     CDS             complement(16477..16560)
                     /locus_tag="OO433_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049474.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16850..17944
                     /gene="gcvT"
                     /locus_tag="OO433_000908"
     CDS             16850..17944
                     /gene="gcvT"
                     /locus_tag="OO433_000908"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6049475.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            17968..18357
                     /gene="gcvH"
                     /locus_tag="OO433_000909"
     CDS             17968..18357
                     /gene="gcvH"
                     /locus_tag="OO433_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6049476.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18476..21349
                     /gene="gcvP"
                     /locus_tag="OO433_000910"
     CDS             18476..21349
                     /gene="gcvP"
                     /locus_tag="OO433_000910"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6049477.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETSISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYL"
     gene            21615..22358
                     /locus_tag="OO433_000911"
                     /pseudo
     CDS             21615..22358
                     /locus_tag="OO433_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(22415..23848)
                     /gene="bglA"
                     /locus_tag="OO433_000912"
     CDS             complement(22415..23848)
                     /gene="bglA"
                     /locus_tag="OO433_000912"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6049478.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEIPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMINAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23893..24204
                     /locus_tag="OO433_000913"
     CDS             23893..24204
                     /locus_tag="OO433_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR6049479.1"
                     /translation="MQPNDITFSQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24368..25027
                     /locus_tag="OO433_000914"
     CDS             24368..25027
                     /locus_tag="OO433_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6049480.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSMCHFLAIYLYIGQ
                     A"
     gene            complement(25223..26203)
                     /gene="ygfZ"
                     /locus_tag="OO433_000915"
     CDS             complement(25223..26203)
                     /gene="ygfZ"
                     /locus_tag="OO433_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6049481.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAADDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26446..26712
                     /gene="sdhE"
                     /locus_tag="OO433_000916"
     CDS             26446..26712
                     /gene="sdhE"
                     /locus_tag="OO433_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6049482.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26693..27100
                     /locus_tag="OO433_000917"
     CDS             26693..27100
                     /locus_tag="OO433_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCR6049483.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(27140..27661)
                     /gene="fldB"
                     /locus_tag="OO433_000918"
     CDS             complement(27140..27661)
                     /gene="fldB"
                     /locus_tag="OO433_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6049484.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27773..28669
                     /gene="xerD"
                     /locus_tag="OO433_000919"
     CDS             27773..28669
                     /gene="xerD"
                     /locus_tag="OO433_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6049485.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28694..29404
                     /gene="dsbC"
                     /locus_tag="OO433_000920"
     CDS             28694..29404
                     /gene="dsbC"
                     /locus_tag="OO433_000920"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6049486.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGN"
     gene            29410..31143
                     /gene="recJ"
                     /locus_tag="OO433_000921"
     CDS             29410..31143
                     /gene="recJ"
                     /locus_tag="OO433_000921"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6049487.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31234..32332
                     /gene="prfB"
                     /locus_tag="OO433_000922"
     CDS             join(31234..31308,31310..32332)
                     /gene="prfB"
                     /locus_tag="OO433_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6049488.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32342..33859
                     /gene="lysS"
                     /locus_tag="OO433_000923"
     CDS             32342..33859
                     /gene="lysS"
                     /locus_tag="OO433_000923"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6049489.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33902..34450)
                     /gene="idi"
                     /locus_tag="OO433_000924"
     CDS             complement(33902..34450)
                     /gene="idi"
                     /locus_tag="OO433_000924"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6049490.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34505..34576
                     /locus_tag="OO433_000925"
     CDS             34505..34576
                     /locus_tag="OO433_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049491.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34573..34698)
                     /locus_tag="OO433_000926"
     CDS             complement(34573..34698)
                     /locus_tag="OO433_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049492.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34700..36148)
                     /gene="uacT"
                     /locus_tag="OO433_000927"
     CDS             complement(34700..36148)
                     /gene="uacT"
                     /locus_tag="OO433_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6049493.1"
                     /translation="MSAIDSQLLSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSVGL
                     SIIQVGIDWAAGGKGNPQYGNPVYSGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDALWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         8796 a         9197 c         9768 g         8770 t
ORIGIN      
        1 tggacatttt tatttcgaaa aagatgcgca atttcatttt attagcgcaa accaataata
       61 ttgcccgggc ggcagaaaaa atccatatga cggcttcgcc atttggtaaa agcattgccg
      121 cgctggaaga acaaattggc tatacgctat ttacccgcaa agataataat atcagcctca
      181 ataaagccgg tcaggaactg tatcaaaaac tgttcccggt ttatcaacga ctttctgcga
      241 ttgataatga aatccataac tcagggcgtc gttcacgaga aattgtgatt ggcatagata
      301 atacttatcc gacaattatt ttcgatcagt tgattagtct cggagataag tatgaaggcg
      361 tgaccgccca gccagttgag ttcagcgaaa acggcgttat cgataatctt tttgaccgtc
      421 agttagattt tattatctcc ccacagcatg tatctgcacg agttcaggaa ctggaaaacc
      481 tgactatcag tgaattacca ccgctgcgat tgggctttct cgtttctcgc cgttatgaag
      541 agcgtcaaga gcaagagctg cttcaggagc tgccctggct acaaatgcgc tttcaaaacc
      601 gggctaactt cgaagcaatg attgatgcga atatgcgacc ttgtgggatc aatcccacca
      661 ttatttatcg tccctacagt tttatggcca aaatcagcgc cgtggagcgc ggacattttc
      721 tgacggtgat cccgcatttt gcctggcgac tggtgaatcc ggcaacgctg aaatattttg
      781 atgcgccgca caggccgatg tatatgcagg aatatcttta ttcaatcaga aatcatcgtt
      841 ataccgccac gatgcttcag catattgctg aagatcgtga cgggacaaac cattaaccca
      901 gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa gacgtggcta agatcgtggt
      961 gaatatgtcc gccgggcgca ttttccagat agcgatgcag ataatcccga tacataggat
     1021 gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg ggaaagaccg cgcagatcgg
     1081 cgatcccttg ttcggtgatg atcactttga cgctgtgttc gctgtgatca acatggctgc
     1141 acattggcac gacggttgag atcttgcctt ctttagcaat cgacggagcc ataaagatcg
     1201 acagatacgc gttgcgttca aaatcaccgc tgccgccgat gccgttcatc agatcgaccc
     1261 cggctacgtg cgttgaattt gcatgcccgt aaatatcaaa ctccaggccg acgttcagag
     1321 cgatgacgcc cagacgacgg atgatttccg ggttattgga aatctcctgc ggacgcaaca
     1381 caatgcggct ggcaaagtaa tccatattgt cgtaaatctt gcgcagggaa tcggccgaga
     1441 ttgtcaggct ggaggcgctg gccccgctga ttttgtcggt ttccagtaaa tgcaccaccg
     1501 attcctgtag cacttccgaa tacatcatga acggaggaat ttccgggttt tcccccagac
     1561 gcgccattac cgcattattg atattgccca cgccactttg cagcggcaga aattccggcg
     1621 gaatacgcca tgcgccattt cctgcaataa gaacgtgacc acgttatcgg caatctgctg
     1681 gcacatggga ttttgcttat ccagcatatt accggcgtcg ggcaagttgg tttccacgac
     1741 ggcgacaatc tttttcggat cgatttgcac atagcgggta ccgacgcgat ccattgcatg
     1801 gaagatcgac acgctattgc gccgcggtgg cgcgccagga atcacaatat ctgccagttc
     1861 tgcaacgcgc ggatcgtgat agtgattaag ttcaatgatc actttcttcg ctcgcagcag
     1921 ccaggtcggc gcattaccga tcccgctggt taaccagact cgaccatccg gtgccagtgc
     1981 cgatgcttca atgacggcaa catcaatgtc gccgaagaaa ccgtaattga ccatttgcgc
     2041 cacttcgctc aaatgcaggt caacgaaact caccgcgccc tgattgatct ttttacgtaa
     2101 accggacgat gtttgatatg gcgcacgcca ggaaacagca tcggcgtcag aaagtacatc
     2161 gtcagcggcg gcgctgattg acgcccccgt cagcaggcga atttgatacg gctttttggc
     2221 ctcatgctgt tcgttagctc tgcgggcaat cgcggtgggt agggctttcg gcgaacccgc
     2281 cggggtaaag ccgctaaatg ccaccatgtc gttatgctgg ataatttctg ccgcttcatc
     2341 ggcggtcatc cttgtccact gagtttccat ttttactcct tcgttcgcag ggattaatga
     2401 ccaacgaaat taggtttacg tttttcgagg aaagcgttca ttccttcctg gtaatcttcg
     2461 ctgtcataaa ccgcgcgtcg catcccctga atacgttcaa attcatcgga gttcatggtg
     2521 tgcgcttcgc ccagtacacg cagctcttct ttgataacag caatggctaa cggcgctttc
     2581 tcagagatgt ggtgcgccat ttgtaaggtg aaatcttcca gttcttccac ttccacaaca
     2641 tggttgagga tgccgacagc cagcgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc
     2701 agctctttga caatgtggaa gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta
     2761 tacgggacgc cgaggtttac aggcgtcatt gagaaggttg aggtactggc ggcgatgatc
     2821 agatcggaac tcatgatcat ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa
     2881 atgatcggtt tcgggaattt ttggatcatg cgggtgattt gacgcaatgg atcatcatag
     2941 gagagcggat cgcgaccgcc agacggcagt tcgtgaatat cgtgacctgc ggagaagact
     3001 ttggatccac tcggcgcgcg caaaatgata cagcgaattt ccggccggtt gagatcgctt
     3061 aacgcctgca taagatcatc aataaagact ttacttaagg cattaagttt tcggccatag
     3121 ttaaactcaa tgaccgccac tttgttgata gtggcaacgt tcacatactg ataagacata
     3181 aaaattcctt taatcaaaat attgcgtctg gataaattca ctgagctgcc gcaggccggt
     3241 gcgcggtgag agcgtattgt ttttgaccgc taaaagcgtc tggcggtaat agcgatcgaa
     3301 atcttcattc gcgaacaggt gattcagtac ttcttcttcg gtctgcttac gcagccattc
     3361 caccgattgt tgttgccgca cttgttgtaa acgaccactg gcagttagcg cggttttgaa
     3421 ctcgatgata gcgtgccaga tctcatcgat tccacgtttt tccagtgcgc tacaagtcag
     3481 aacccgtggc tgccattcgt cgtatttacg tcgcagaata tgcagggcac tctcgtacat
     3541 atgccgggca atggcgacat tggtatggtt atcgccatcg tctttgttga taacgatcag
     3601 atcagccact tccatcagcc cttttttaat gccctgcaga tcatcgccgc caccggcaat
     3661 ttgcaacgag ataaaacagt ccaccatgcg ggcgacttct gtttccgact gcccgacgcc
     3721 aaccgtttcg acaatcacta cgtcataacc cgccgcttcg cataacagca ttaattcccg
     3781 cgctcgctga ctggcaccgc ccagatgacc ggaggatggt accgggcgaa taaacgccgc
     3841 ttcggcacgc gccaggtcat tcatgcggat tttatccccg agaatgctac cgccagtgac
     3901 cgggctgctg ggatcgaccg caataaccgc gacctttaat ccctctcgaa tcaacaacat
     3961 gccaaaggcc tcaagaaagg ttcttttccc cgcgctgggg gtgccggtaa cgcccagtcg
     4021 cagggtgtta ccgcagtacg gcataatggc atcaagcagc tgcgtactta gtgcctgatg
     4081 acgcgggtga cggctttcca ccagcgtcat ggcctgggcg agtgtggcac gctcaccctg
     4141 acgtaagcgg cgaatacttt ctgccagcgt ggcttcatta atcatgatgc tggcttatca
     4201 gattcagtac gtcgcgcaca ctgtcgagca taggtgtacc tggaccataa atcgccgcca
     4261 cgccgcgctc ttgcaggaag gcgtaatcct gcggtggaat gacgccaccg gcgactacgc
     4321 agatatcttc gcgtccccat tttttcagcg cctcgaccag ttccgggatc agcgttttat
     4381 gaccggcagc cagtgaggat gcgcccacta cgtgaacgtc attttctact gccaggcggg
     4441 cgatctcttc aggtgtagag aacatcgggc ttaaatctac gtcgaaaccg agatcggaat
     4501 aggcgctggc gatcactttc gagccacgat cgtgcccatc ctggcccatt ttggcgatca
     4561 gaatgcgcag gcgacgacca ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat
     4621 cgaactcgga ggccgatttc tcagattgat gatagctttg cgcaatcacg ccggtaacac
     4681 actggcttgg caccagataa cggtcgaaag cggcttccag cgcatcggaa atttcaccca
     4741 gggtggcgcg aacgcgagcg gcattaacag cggcagccag caggttttcg ttatgctgtg
     4801 cggcgtgagt cagggcgttc aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa
     4861 tgcgttccag cgaagcaatt tgctcgttac gcaccatcac gttgtcgatc tcaagtacat
     4921 cggtttcgtc ttcgtgatcc agtttgtact tgttgacacc aacgatgaca cgtttgccct
     4981 ggtcgataag cgactgttcg cgcgctgagg cctattcgat cattcgtttt ggcagacctg
     5041 cttcgatcgc tttcgccata ccaccggctt cgtcgatctg ttggataata gctctggctt
     5101 gtttgacgat ttgatcggtc agcgactcaa tgtaatagga tccggccagt ggatcgacgg
     5161 tgcggcagag ttctgattct tcctggatga tgatctgggt gttgcgggca atgcgtgctg
     5221 agaaatcggt aggcaaacca agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac
     5281 cgcccagcgt cgcagccagt gcttcaatgg tggtgcggat aacgttgtta tacggatcct
     5341 gttcagtcag gctccagcct gaggtctggc agtgggtacg cagcgccagt gatttcgggt
     5401 cctgtgcgcc aaatccactg accgcttcgc tccataaata acgtgccgca cgcaacatgg
     5461 cgacgttcat aaacagatcc atgccgatgc cgaagaagaa cgacaggcga ggagcgaagt
     5521 catcaatttt cagtccggca gagattgctg ctttgatgta ctcaatccca tcagcgagcg
     5581 taaatgctac ctgctgcacg cagttggcac ccgcttcacc catgtggtaa ccgctgatac
     5641 tgatggtatt aaatcgcggc atgttgccgg aacaccaggc gatgatgtcg gcgataatgc
     5701 gcattgacgg ttttggcggg taaatatagg tgttgcggca gaggtactct ttgaggatat
     5761 cgttctgaat ggtgccggtc agtttatcag gtgtaacacc ttgctcttcc gcggcgacga
     5821 tataaaacgc cagtactggt agcactgcgc cattcatggt catcgaaacc gacattttat
     5881 ccagcgggat ctggtcgaac aggactttca tatcttccac ggtgtcgata gcgacgcccg
     5941 ctttgccgac gtcgcccgcc acgcgcgggt tatcggagtc gtagcctcgg tgggtggcaa
     6001 ggtcaaacgc aacggaaaga cctttttgcc cggcggccag gttacggcga taaaaagcgt
     6061 tggactcttt tgctgttgaa aaaccagcat actgacggat ggtccacggt tgggcggtat
     6121 acatagtggc acgcgggcca cgaacgtagg gcggcaaacc aggaagggta cctgtcacct
     6181 ccagattatc gagatcggct tcggtataca gcggcttgat ggtgatccct tccgcggttt
     6241 gctgaaccag cgagtcgaca gttttctccc gacggctcaa ttccttgttg gcaagctgtt
     6301 gccactcctg cacgttagac attatttgct ccgtaaaacg caatcaaaat gagggtattt
     6361 gcgcctacgg tgagcttttt acgtgggttt gtcgccagca aaaaaaatgc attcagacgt
     6421 cgttttttgc cgcctgagat ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa
     6481 gtgaggacaa cgcctgatat gtgctatcag gcattatttg atggattaat cctgacgaag
     6541 gactttgtga ccataatcga gcaacgcatc agtgacttta cgcatcatgc ggctttcagg
     6601 agcaaagcgg tgccagtaga gcatccggcg ttgaaatagc cctggcgtta agtcaatcag
     6661 ttcaccgctg gctagttctt tctcgatttg caggtgcggg atcatacagc aggtggtgcc
     6721 ctggcgagca agttgcacga acgcttctga agaattaacg atatggcagg gcacgctgcc
     6781 tggaggcaga tcgaagtttt gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa
     6841 ggcgaccaca ggtgctttca ataatgccga acgcgttacg ccgttaggga aatatttttc
     6901 ggcaaacggt tttgagctga cgaacagata gtcaagcgca ccgagcttat cgacaaggca
     6961 actcggcagc gcctgatgtt gaatactcac cgcgccgacc acttcgccgc ggcgcagacg
     7021 ttcctgagta cgggtttcat cttctacctg caagttgagg cggataggcg aatcagccaa
     7081 tacaggagcc agtgccggaa gcaaccacgt cgccagactg tcggcgttga ccgccagtga
     7141 aagcagcagc ggagtcgaac cggtttgttc atcgcccagc cactcttctt ccagcaactc
     7201 cacctggcgc agcagtgcca gcagtttttg cccttgttcc gtcgggcgcg gcggtacggt
     7261 acgcaccaac agcggctgcc cgaacatatt ttccagttgc ttaatgcgct gtgagacggc
     7321 tgattgtgta atgcacagct tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc
     7381 atccagtgcc tgtaatgttc tgtagtccgg gcgtttcatt gctgttgtct gctcctggta
     7441 aatgcttttt cctgcactat gacacaattt tgtgtcaggt tgcatatacc gcagccgtcg
     7501 gcgggtaaca tttgtatcaa tggttaaacg cgcagcttaa aagatcaggg atttgcgcta
     7561 ttgcatttcg cttagcgact tcgcgccctg gttatgttga atggcgtggc gttattgcct
     7621 ctgttcgcac cgtggagcgg catacccaga ccgttgtatg ctttatttgc ggggcgaaag
     7681 gggatgcccg ccattgcgcg atattttatg gatgagttaa ccacccggct tgccaacggg
     7741 gtctgaatcg ctttttttgt atataatgcg tgtgaaattt cataccacag gcgaaacgat
     7801 catgacgcag gatgaattga aaaaagcagt aggatgggcg gcacttcagt atgttcagcc
     7861 cggcaccatt gttggtgtag gtacaggttc caccgccgca cactttattg acgcgctcgg
     7921 cacgatgaaa ggccagattg aaggggccgt ttccagttca gatgcttcca ctgaaaaact
     7981 gaaaagcctc ggcattcacg tttttgatct caacgaagtc gacagccttg gcatctacgt
     8041 tgatggcgca gatgaaatca acggccacat gcaaatgatc aaaggcggcg gcgcggcgct
     8101 gacccgtgaa aaaatcattg cttcggttgc ggaaaaattt atctgtattg ccgacgcctc
     8161 caagcaggtt gatattctgg gtaaattccc gctgccagta gaagttatcc cgatggcacg
     8221 tagtgcagtg gcgcgccagc tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt
     8281 ggtgaccgat aatggcaacg tgatcctcga cgtccacggc atggaaatcc ttgacccgat
     8341 agcgatggaa aacgccataa atgcgattcc tggcgtggtg actgttggct tgtttgctaa
     8401 ccgtggcgcg gacgttgcgc tgattggcac acctgacggt gtcaaaacca ttgtgaaatg
     8461 atctgacggg ggaacctccc ccgttaaaaa aattctcttc attaaatttg gtgacatgtg
     8521 tcacgttttt acctggctat tgtcgattgc tctaaataaa tcctctaaac cagcatattc
     8581 aaccaagaaa tacctttgcg tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc
     8641 cgcaatatta ttttttgata tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa
     8701 gacaggattg ggtaaatggc aaaggtatcg ctggagaaag acaagattaa gtttctgctg
     8761 gtagaaggcg tgcaccaaaa ggcgctggaa agccttcgtg cagctggtta caccaacatc
     8821 gaatttcaca aaggcgcgct ggatgatgaa caattaaaag aatccatccg cgatgcccac
     8881 ttcatcggcc tgcgatcccg tacccatctg actgaagacg tgatcaacgc cgcagaaaag
     8941 ctggtcgcta ttggctgttt ctgtatagga acaaaccagg ttgatctgga tgcggcggca
     9001 aagcgcggga tcccggtatt taacgcaccg ttctcaaata cgcgctctgt tgcggagctg
     9061 gtgattggcg aactgctgct gctattgcgc ggcgtgccgg aagccaatgc taaagcgcac
     9121 cgtggcgtgt ggaacaaact ggcggcgggt tcttttgaag cgcgcggcaa aaagctgggt
     9181 atcatcggct acggtcatat tggtacgcaa ttgggcattc tggctgaatc gctgggaatg
     9241 tatgtttact tttatgatat tgaaaataaa ctgccgctgg gcaacgccac tcaggtacag
     9301 catctttctg acctgctgaa tatgagcgat gtggtgagtc tgcatgtacc agagaatccg
     9361 tccaccaaaa atatgatggg cgcgaaagaa atttcactaa tgaagcccgg ctcgctgctg
     9421 attaatgctt cgcgcggtac tgtggtggat attccggcgc tgtgtgatgc gctggcgagc
     9481 aaacatctgg cgggggcggc aatcgacgta ttcccgacgg aaccggcgac caatagcgat
     9541 ccatttacct ctccactgtg tgaattcgac aacgtacttc tgacgccaca cattggcggt
     9601 tcgactcagg aagcgcagga gaatatcggc ctggaagttg cgggtaaatt gatcaagtat
     9661 tctgacaatg gctcaacgct ctctgcggtg aacttcccgg aagtctcgct gccactgcat
     9721 ggtggtcgtc gtctgatgca catccacgaa aaccgtccgg gcgtgctaac tgcgctgaac
     9781 aaaatcttcg ccgagcaggg cgtcaacatc gccgcgcaat atctacaaac ttccgcccag
     9841 atgggttatg tagttattga tattgaagct gacgaagacg ttgccgaaaa agcgctgcag
     9901 gcaatgaaag ctattccggg taccattcgc gcccgtctgc tgtactaatt ccccttctct
     9961 gaaaattaac gggcaggtca ctgacttgcc cgttttttta tcccttctcc acaccgttgc
    10021 cgcgttgcgc attctacttt gcgagtctgc tccagaaaat aaatcaaagt cagatcaatg
    10081 cgttgtgttt atttaatggg gatttacgat ggcgggacag tatggggctg taacggtaaa
    10141 gccctcaccg aagcgagggc ttaaaggaga agggttatga tgcgacttgt catcatactg
    10201 attgtactgt tactgataag tttcggagct tattaacagt cagtctctgg ggaggagtca
    10261 tcctccctta cccttactca ctaaattagg tcaaagaatc aacgatgtca atcagggcga
    10321 tgcggggtgc atcgccctga accgaacttt ttgatagcca cttcatcgcc cacgtttact
    10381 gccgtaatag atcctttttc ccaaaaatta tctctgtttt gcatggggtt tttcgaacac
    10441 gctcgaagaa aagatttaaa ggtagatcaa tgcgttgtgg ttatttaaag agagcttacg
    10501 atagctgggc agcatggggc tgtaacggta aagccctcac cgaagcgagg gcttgaagga
    10561 gaagggttat gatgcgactt gccatcatac tgattgtact gttactcata agtttcccag
    10621 cttattaaca gtcagtcaca ggggaggagc aatcctccct tacccttact cactaaatta
    10681 ggttaaagaa tcaacgatgt caatcagggc gatgcaggtg tatcgccctt accactccca
    10741 gactttcgac ggtgtaacca ccgcaggaag agggatatcc cactcttcaa cggggagttt
    10801 ttccaccaac tgacaatcat gcgcataacc caccggttgc gttttatagt gctgccagtt
    10861 ttgtaaggtc cgatcataaa aaccaccgcc cattcccagg cgctgaccgt actcatcaaa
    10921 ggcgaccagc ggtgtgatca gcacgtctaa tcgggaaagg ggtagcacgt cacgcacatc
    10981 cagttttggc tcatggattt tcaacctgtt catcaccagt tcgctttgcg gatggtaatt
    11041 caggaacagc aaattaccag cactaaaggg atgcaaaact ggaagatata cgcgcttacc
    11101 ggcgcgccag agttgttcta ttagcggctg ggtgtcgagt tcaccatcaa aagagaggaa
    11161 tacagcgacc gtatgcgcca tcaccaccgg gggataagtc atcatccggg tagcggcttg
    11221 ttgacccatt tcctgctgtt gttccggcgt taacgcacga cgacgttgcc gaatcatttt
    11281 gcggatttct tgtcgggata atgtcagtgg gagttctggt agttgcgtca tggctggtgc
    11341 cagataagaa gggaatctcc gagatgccgc cgcaggctgt aacccttgaa cccttggttc
    11401 aaggtgaatg tgtcgtcgca gttttaaggc ttctcggacg gaccgagcat gctcaccaac
    11461 cgcggagcgc cacattcttg tggtatgaaa tatcggctca ggggactggc ccgcttgcga
    11521 acatctcaga gaaattttgt cttcacggtt actctaccac agtaaaccga aaagtgtcat
    11581 tcaaagtttt ggttagtttt ttcggtgatg cgaccttgtt caagtaacgc ttgttctatg
    11641 gtctgctgca gcatccgaat acgctgttcc atacttgccg cgtagtcacg agtcttcgct
    11701 ttttcttgcg ctaactcata gctgatattc aatgcggcaa tgaagaccaa ctgttcagta
    11761 tttgtgactc tagtgcgttc tttcagatct tgcaaccgtt ggttcagatc gtccgctgcc
    11821 tgattcaacg catccctttg gtcaggcggg cagttcacac gcagtgaacg gccaaaaatt
    11881 tggatatcga cgggttgtgc agacatgcca ccttcctgct gattgactgc gctgccttcg
    11941 tctttagacc catggtctgc gaaggggcga cactatagct accctgatga gaagagacaa
    12001 gcccttttct ggtccaccag ggcccaaagt ggtagcatat catgaatatt cctccctttg
    12061 acgacgaatg cttatgtcta tacagaacga aatgcctggt tacaacgaaa tgaaccagta
    12121 tctgaaccaa caagggacgg gtctgacccc tgccgagatg catggtttaa tcagtgggat
    12181 gatatgtggc ggtaacgatg acagctcatg gctgccgcta cttcacgacc tgacgaacga
    12241 aggcatggct ttcggtcatg agctggcaca ggcactgcgt aaaatgcact ctgccaccag
    12301 cgatgccctg caggatgacg gcttcctttt tcagctttat ctgcctgatg gcgatgatgt
    12361 cagcgttttc gatcgggctg atgcattggc aggttgggtc aatcacttcc tgcttggtct
    12421 tggcgttacg caaccgaagc tggataaagt gaccggcgaa accggtgaag ctatcgacga
    12481 tctgcgtaac attgcgcaac tgggttacga cgaagacgaa gatcaggaag agcttgaaat
    12541 gtcgcttgaa gagatcatcg aatacgttcg tgttgccgcg ctgttatgcc acgacacctt
    12601 tactcatccg caaccgaccg cgccagaagt acaaaaaccg actctacact aaaaacaaaa
    12661 aacgtaagga gagtgttatg agtgagatat cccggcaaga gtttcagcgt cgccgtcagg
    12721 ccctggtgga gcaaatgcaa cccggcagcg ccgcgctgat ttttgctgca ccagaagtaa
    12781 cacgtagcgc cgacagcgaa tacccctatc gtcagaacag tgacttctgg tacttcaccg
    12841 gctttaacga accggaagcg gtgctggtgc tgattaaaag cgatgacact cataaccaca
    12901 gcgttctgtt taaccgcgtt cgcgacctga cggcggagat ctggtttggc cgtcgcttag
    12961 gccaggatgc cgcgccagag aaactgggcg ttgaccgcgc actggcattc agcgaaatca
    13021 atcagcaact ttatcaacta cttaacggcc tggatgtggt ttaccatgcc cagggcgaat
    13081 atgcatatgc tgatgaaatc atgaacagtg cgctggaaaa actgcgtaaa ggttcgcggc
    13141 aaaatctcac cgcaccggca acgatgatcg actggcgtcc tgttgttcat gaaatgcgcc
    13201 tgttcaaatc gccagaagag attgccgtac tccgccgcgc gggagaaatc accgccatgg
    13261 cacatacacg ggcgatggaa aaatgccgtc cgggaatgtt cgagtaccat ctggaaggcg
    13321 aaattcacca cgaatttaac cgccacggtg cgcgctatcc gtcctataac accattgtcg
    13381 gcagcggtga aaacggctgc attctgcact acaccgaaaa cgagagtgaa ctgcgcgacg
    13441 gtgacctggt gttgattgac gcgggttgtg aatacaaagg ttacgcgggc gatattaccc
    13501 gcaccttccc ggtcaacggc aaattcaccc aggcccagcg tgaaatctac gacattgtgc
    13561 tggagtctct cgaaaccagc ctgcgcctgt atcgtccggg aacttccatt ctggaagtca
    13621 ctggtgaagt ggtgcgcatc atggttagcg gcctggtaaa actcggcatt ctgaaaggtg
    13681 atgttgatga actgatcgct cagaatgccc atcgtccttt ctttatgcat ggccttagcc
    13741 actggttagg gctagatgtc catgacgttg gcgtttatgg tcaggatcgc tcgcgcattc
    13801 tggaaccggg catggtactg accgtagagc cagggctgta tattgcgccg gatgcagaag
    13861 tgccagaaca atatcgcggt atcggcattc gtattgaaga cgacattgtg attaccgaaa
    13921 ccggtaacga aaacctcacc gccagcgtgg tgaaaaagcc ggaagaaatc gaagcgttga
    13981 tggctgctgc gagaaagcaa tgagcgtaat catcgtcggt ggcggcatgg cgggcgcgac
    14041 gctggcgctg gctatttccc ggttaagtca cggggcgctg ccggtacatt tgattgaagc
    14101 gactgcgcca gagtcacatg ctcatccggg ctttgatgga cgagcgatag cgctggcggc
    14161 gggtacctgt cagcaactgg cgcgcatcgg cgtctggcaa tctctggcgg attgcgcaac
    14221 tgccatcacc accgtgcatg tcagcgatcg tggtcacgct ggatttgtca ccctcgccgc
    14281 agaagattac caactggcgg cgctgggaca ggttgtcgaa ttgcacaatg tcgggcaacg
    14341 gctgtttgca ttgctgcgta aagcacctgg cgtaacgctg cattgccctg atcgcgtggc
    14401 taacgttgcc cgtactcaga gtcacgttga agtgacgctg gagagtggcg agacgctgac
    14461 gggccgcgtg ctggtagcag ctgatggcac ccattcagcg ttagccaccg cgtgcggcgt
    14521 tgactggcag caggagcctt acgaacaact ggccgtgatt gccaacgttg cgacttccgt
    14581 tgcgcacgaa ggccgcgctt ttgaacgctt cacgcaacat ggcccgctgg cgatgttgcc
    14641 gatgtctgac ggacgctgtt cgctggtctg gtgtcatcca ctggaacggc gcgaagaggt
    14701 gctgtcgtgg agcgacgaga agttttgccg tgaactccag tcggcctttg gctggcgact
    14761 tggaaaaatt acccacgccg gtaaacgcag tgcttatccg ctggcgttaa cccgcgccgc
    14821 cagagctatt acccatcgta ccgtgctggt gggcaatgcg gcgcaaactc tgcacccgat
    14881 tgccgggcaa gggtttaacc tcggtatgcg agatgtcatg agccttgcgg aaaccctgac
    14941 tcaggcgcat gagcgcggag aagacatcgg tgattacggc atattgtgcc gctatcagca
    15001 gcgtcgacag agcgatcgcg aagcaaccat cggcgtcacg gacagcctgg tacatctttt
    15061 tgccaaccgt tgggcaccgc tggttgtcgg gcgcaacatc ggactgatga cgatggaatt
    15121 attcaccccg gcacgcgatg tgctggcgca gcgcaccctc ggttgggtgg cgcgttgaag
    15181 gcggttttaa ggaataaaca atgcaatgtg ttgatgtagc cattgttggt ggcggcatgg
    15241 tggggctggc ggttgcctgc ggcttacagg ggagcggctt acgcgttgcc gtactggagc
    15301 agcgcgtacc ggaacctctg gcggcggatg caccaccaca actgcgcgtt tcggctatca
    15361 atgccgccag cgaaaaatta ctcacccgtc ttggcgtctg gcaggacatt ctctctcgta
    15421 gggccagttg ttatcacggt atggaagtgt gggacaaaga cagttttggt cacatttcgt
    15481 ttgacgatca aagcatgggc tatagccatc ttgggcatat cgttgaaaac tcagtgattc
    15541 actacgcgct gtggaacaaa gcgcagcagt cgtcagatat cactctgttg gcccccgcag
    15601 aattacagca ggtcgcctgg ggagaaaatg aaactttcct gacgctgaaa gatggcagca
    15661 tgttaacggc gcgtctggtg attggcgcgg acggtgctaa ttcctggttg cgcaacaaag
    15721 cagatatttc gctgactttc tgggattatc agcatcacgc gctggtagcg accattcgca
    15781 cggaagaacc gcatgatgcg gtggcgcggc aggttttcca tggcgaaggc attctggcct
    15841 ttttaccgct tagcgatccg catctttgct caattgtctg gtcactgtcg ccagaggaag
    15901 cgcagcggat gcagcaggca agtgaagatg aatttaatcg cgcgttaaat atcgcttttg
    15961 ataatcgcct gggcttatgc aaggttgaga gcgcgcgtca ggtgttccca ctgacggggc
    16021 gttatgcgcg ccagtttgcc gcgcaccgtc tggtgctagt gggcgacgcc gcgcatacca
    16081 ttcacccgct ggcggggcag ggagtaaatc tcggctttat ggatgctgca gagctgattg
    16141 ctgaactgaa acggttgcat cgtcagggga aagacatcgg gcagtacatt tacctgcgcc
    16201 ggtatgagcg tagccgcaag cacagtgcgg cgctgatgct ggctggtatg cagggattcc
    16261 gcgatctgtt ttccggtacc aatccggcga aaaaactgct gcgtgatatt ggtttgaaac
    16321 tggccgatac gcttcctggc gttaaaccac aacttatccg ccaggcaatg ggattaaacg
    16381 atttgcctga atggctgcgt taaaaatttc tcttctgctg tttatttgat acccatcaca
    16441 ctttcatctc ccggtttttt cgccgggaga ttttcctcat ttgaaataaa ctaatttcac
    16501 ctccgttttc gcattatatt ttctaatgtc attatttttt gatttagtgt tttttgacat
    16561 ttttttagct cttaatattg ccttattcaa attagatttc taatggcatc atctttatat
    16621 agaaactggt gtgttttttg gttttttatt ctgtcgcgat tttggcgttt tttaaccata
    16681 agctaatgtg atgatcaatt ttaccttatg gttaacagtc tgtttcggtg gtaagttcag
    16741 gcaaaagaga acgattgcgt tggggaccgg gagtggctcc gatgctgggt ttcgtggtga
    16801 taatttcacc atgaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    16861 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    16921 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    16981 cggaatgttt gatgtgtcac atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    17041 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    17101 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gacctcatcg tctactactt
    17161 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    17221 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    17281 gattgccgtg caagggccga atgcgcaggc aaaagctgcc acactgttta atgacgccca
    17341 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggcgggcg atctgtttat
    17401 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    17461 ggccgatttc tggcgtgcgc tggtggaagc gggtgttaag ccatgtggct tgggcgcgcg
    17521 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    17581 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccggcag atcgtgactt
    17641 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    17701 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    17761 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    17821 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    17881 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    17941 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    18001 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    18061 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    18121 gttagcgcgg gcgatgactg cgcggttgcc gaatcagtaa aagcggcgtc agacatttat
    18181 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc tccggaactg
    18241 gtgaacagcg aaccgtatgc aggtggctgg atctttaaaa tcaaagccag cgatgaaagc
    18301 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga cgagtaacgg
    18361 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    18421 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    18481 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    18541 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggcgca caatcgttaa acgcgctgac
    18601 cggccagatt gtaccgaaag atattcagct tgcgaccccg ccgcaggttg gcgcaccggc
    18661 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    18721 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    18781 gctggaaaat ccgggctggt ataccgcgta cactccgtat caacctgaag tctcccaggg
    18841 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    18901 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    18961 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccgatg tgcatccgca
    19021 aacgctggat gtggtccgta ctcgtgccga aacctttggt tttgaagtga ttgtcgatga
    19081 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    19141 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    19201 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    19261 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    19321 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    19381 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    19441 gcaacatatc cgccgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    19501 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    19561 ccgcattcac cgtttgaccg atatcctggc ggcaggcctg caacaaaaag gtctgaagct
    19621 gcgccatgcg cactatttcg acactctgtg tgtggaagtg gccgacaagg cgggcgtact
    19681 ggcgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg cggttgggat
    19741 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagcg tgctgctggg
    19801 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    19861 tattcagcct gcgatgctgc gcgacgacga aatcctcact catccggtgt ttaatcgcta
    19921 ccacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    19981 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    20041 gatcccaatc acctggccgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    20101 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    20161 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    20221 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    20281 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    20341 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    20401 gggcgataac ctctcctgta tcatggtgac ttatccctct acccacggcg tgtatgaaga
    20461 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    20521 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    20581 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    20641 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    20701 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    20761 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    20821 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    20881 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    20941 gaaagaagaa accagcatca gcgagctgga tattgccaag cgcctgatcg actacggttt
    21001 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    21061 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    21121 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    21181 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    21241 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    21301 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa tacctgtaat tcactgattc
    21361 gactattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    21421 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    21481 gcgcgaatat ttaccctcac cccggccctc tccctgaaag ggcgagggga aaagcgtgcc
    21541 aacattgaag attgagccag tttgttagca atctcaaaga tacgtcaacg aattaatttt
    21601 tctcggaaaa acaaatggct atagcacttg tgaccggtgg cagtcgcggc atcgggcggg
    21661 caactgcatt actgttggcg aaagaagagt atacggtggc ggttaattat caacaaaacc
    21721 tccatgcggc gcaggaagtg gtgaacttaa tcacgcaagc cggtggcaag gcattcgtgc
    21781 tccaggcgga tatcagcgac gaaaatcagg tcatcgcaat gtttacagca atcgatcagc
    21841 acgatgaacc gctagcagcg ctggtcaata acgccgggat cttgtttacc cagtgcaccg
    21901 ttgaaaacct taccgcagag cgaatcaacc gagtactttc caccaacgtg acgggatatt
    21961 ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc agtggcggcg
    22021 ctatcgtcaa tgtctcttcg gtggcctcac ggttgggttc gccaggggaa taggttgatt
    22081 acgcggcatc gaaaggggcg attgatacgt taaccaccgg actatcgctg gaagtcgccg
    22141 cgcaggggat ccgcgttaac tgcgtgcggc cagggtttat ttataccgaa atgcacgcca
    22201 gcggcggcga gcttggacgc gtcgatcgcg ttaagtcgaa catccccatg cagcgtggtg
    22261 gacaggcaga agaggtcgcg caggccattg tctggctact aagtgataaa gcctcttacg
    22321 tcacgggaag ttttatcaat ttggcgggcg ggaaataaaa cagggaagtt gtctgaccgg
    22381 atgcaacaag tattgcatcc ggtacttcat cgacttaaag cttctcgccg ttgctggcaa
    22441 tcacctcttt gtaccagtta aagctcttct tacgtgaacg cgacatatcg ccagtaccgt
    22501 cgtcatgttt attcacatag ataaagccgt agcgtttgct gtactgcccg gtagtgaatg
    22561 acacgcagtc gatgcagccc cacggtgtgt agcccatcag atccacacca tcgtaagtca
    22621 ctgctttttt catctcttca atatgggcgc gcaggtagtc aatgcggtag tcgtcgttga
    22681 tgctgccatc ttcttccact ttgtcgtaag cgccaaaacc gttttcgaca ataaacagcg
    22741 gcctctgata acgctcatac agttcgcaaa gggcatagcg cagacctact ggatcaatct
    22801 gccagcccca gtcagacgct ttaacatacg ggttcggtac gctgccttca aaaccagaga
    22861 tcgcatcgcc ggtgccgcct tcggccttca ctgcgttgat catgtaatag ctgaaaccaa
    22921 gataatcgca ggtgccttca cgcagtacat ccagatcgcc atcttccatt ttgatgttaa
    22981 atccgcgacg ctcccactcg ttcaacacat aggacgggta atagccgcgt agctgcacat
    23041 cggtaaagac gtagcgttcg cgcatcgact cctgagcgaa catcacatcg tccgggttac
    23101 aggagtaagg atagagcggc accatcgcca gcatacagcc gactttcatc tccgggttaa
    23161 tgcgacgcgc ggctttcact gctagggcgc tggcgacaaa ctggtgatgc agcacctggt
    23221 acatcgtctc ttccgggttg tcatgctcgg tatacaccac gccggagcag cagtaaccaa
    23281 acagcggcgc acgccagtta cgctggttgt ttatttcgtt gaaggtcatc cagtatttga
    23341 ctttatgctt gtagcgttcg aataccactt ccgcgaaacg gacaaagaaa tcaacgactt
    23401 tacggttggt ccagctaccg tattgctgca ccagatgcag cggtatttca aagtgggaga
    23461 gggttatcac cggttcgatg ttgtatttca gcagttcatc gaacagagag tcgtaaaact
    23521 tcagcccttc ttcgtttggc tgagcttcat caccttttgg aaaaatacgc gtccaggcga
    23581 tggaagtccg aaaacatttg aagcccattt cggcaaatag cttgatgtct tccttgtagt
    23641 gaccataaaa atcaacggct tcatggtttg gatagtattt tcctggcaag acttctttgg
    23701 tgatttcgcg cggcacgccg tgtgcgccac cggtcagaac gtcacaaatg ctcggtcctt
    23761 tgccgccttt gttccagccg ccttcgacct gatgagcggc aacggcgcca ccccataaga
    23821 aatctttcgg taaggtgagt tttttcacta tcataggctc cagatgagtc aattcttaca
    23881 ggaaatagta ccatgcagcc aaacgacatc actttttctc aacgtttcca ggatgacatt
    23941 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    24001 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    24061 gcaacctcaa ccgtaacgct ggatacgctg acggaaaaac atgcagagca ggaaaatatg
    24121 accctgactg aactgataaa ggtcattgcc gacatctatc ccggtcagac acagttttat
    24181 gtgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttctcattt
    24241 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    24301 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctccgga
    24361 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    24421 caacagcgtc agtcacggca ttgggttggt gtttggtatc gttgggctgg tgttgctact
    24481 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagctaca gcctctatgg
    24541 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    24601 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    24661 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    24721 tatctggagc ctggcattgc tggggattct gtttaaactg accatcgcgc accgattcaa
    24781 aattttatct ctggtgacct atctggcgat gggctggctg tcgctggtgg taatttatga
    24841 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    24901 ttcgctcgga gtgattttct atgtctgcaa acgcattccc tacaatcatg ccatctggca
    24961 cggcttcgtg ctcggcggca gtatgtgcca ctttctggcg atctatttgt atattgggca
    25021 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg
    25081 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    25141 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    25201 atgcctgatg cgatacagag atttactctt cgagtgaata cggcagcggc tcgatatgca
    25261 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    25321 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    25381 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    25441 cgctacctgc cagcaaccag agcgcacgtt tattggcacc acggaatttt gctcgcgcca
    25501 ccatctcttg tccggtataa cagcctttct taaaactgat accgcccagc gcctggagat
    25561 tggtcgcctg tgggataaac tgcccgctgt tcgcggcatc aatcaccggg aaacccgctt
    25621 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    25681 cggtcagcat attggcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    25741 caaaccatag caaagtggtc gcgccttctt taactacctg tttttctctc gaaggtagtt
    25801 cactaaagag attggcgagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    25861 gctcgtcgtc tgccgcgatg gtcactttag agaacaccgc atattttttc agttcagtca
    25921 gctgcggttc acgcacgctg cgccgttcaa tccacgcaaa gccatcgccg tcgcggaaca
    25981 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    26041 tttctgtcat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    26101 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    26161 cagaagccgt aggctgacgg ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    26221 cttaacggcg tattaataac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    26281 tacgcgatct gtgccttctg cgaggcgact tccaaaaaaa tgaagctctt gaattgttgt
    26341 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    26401 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    26461 aaagcccgca ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    26521 ccgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    26581 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    26641 gatgcagaac tggaaatgat ggtccgactc atccagacac ggaaccggga acgtggtcct
    26701 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    26761 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    26821 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggcgta ttaatgctcg
    26881 ccagggggaa attcgattgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    26941 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    27001 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagctg aatggcggga
    27061 tttacggcgg attttgttgc aacaagagac gcaaagataa atatgacggc aaagcatatg
    27121 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    27181 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    27241 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    27301 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    27361 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaacaa
    27421 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    27481 tttcgccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    27541 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    27601 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    27661 taacgtaagc ttctcgattc tgctgtggct tttgcggcta gtgtatcaga atcgctttag
    27721 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    27781 ggatctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    27841 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    27901 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    27961 agagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    28021 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    28081 gaaattgcca cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    28141 ggcaccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    28201 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctacg
    28261 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttaggtga
    28321 agaggccgtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgttgaatgg
    28381 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    28441 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    28501 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    28561 ggtgcagatg ctactgggcc acagcgatct ctccaccacg caaatttata cgcatgtcgc
    28621 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    28681 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    28741 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcattaaaag
    28801 cagcgatatt cagccagcac ccgtggctgg catgaagact gtgctgacta acagcggtgt
    28861 gttgtacatc accgatgacg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    28921 cgcggctccg gtcaatgtca ccaataaaat gctgctaaag cagttgaatg cgcttgaaaa
    28981 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    29041 tacctgtggg tactgccaca aactgcatga gcaaatggca gactacaacg cgctggggat
    29101 caccgtgcgt tatcttgctt tcccgcgcca ggggctggag agccaggctg agcaacaaat
    29161 gaaagccatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggctggtaa
    29221 agctgccact ccggcaagtt gcgatatcga tattgccgat cattacgcac ttggcgtcca
    29281 gcttggcgtt agcggcactc cggcaattgt gctgagcaat ggcacacttg ttccgggtta
    29341 ccagccgccg aaagagatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    29401 ttaattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    29461 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    29521 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    29581 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    29641 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    29701 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    29761 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    29821 aacggtattt cctcccatgc gggggttgaa cacgctcgct cgttgggcat tccggttatt
    29881 gttaccgatc accatttgcc aggcgaaaca ttacccgcgg cggaagcgat cattaaccct
    29941 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    30001 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    30061 gcaattccta acctggcaga actgctggat ctggtcgcgc tggggacagt ggcggacgtc
    30121 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    30181 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaaccgtga tgcacaaaaa
    30241 ctcgccgcca gcgatttagg ttttgcgctg gggccacgtc tcaatgctgc cgggcgactg
    30301 gacgatatgt ccgtcggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    30361 ctggcaaatg aactcgatgc gctaaatcag acgcgaaaag agatcgaaca aggaatgcag
    30421 attgaagcct tgaccctgtg cgagaaactg gagcgaagtc gcgacacgct acccggcggg
    30481 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgcatc
    30541 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgatgg tacgctgaaa
    30601 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    30661 ctctaccccg gtatgatgct gaagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    30721 gaagaggata aattcgaact ctttcaacaa cggtttggcg agctggttac cgagtggctg
    30781 gacccttcgc tattgcaagg cgaagtggtg tcagacggcc cgttaagccc ggccgaaatg
    30841 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    30901 ccgctgtttg atggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    30961 aaagtcatgg tcgaaccggt cggcggcggt ccgctgctgg atggtattgc ttttaatgtc
    31021 gataccgccc tctggccgga taacggcgtg cgcgaagtgc aactggctta caagctcgat
    31081 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    31141 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    31201 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    31261 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    31321 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    31381 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    31441 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    31501 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    31561 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgcta
    31621 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    31681 gcgtatgtat ttgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    31741 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    31801 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaagagcc cgtttgactc
    31861 cggcggtcgt cgccacacgt cgttcagctc cgcgtttgtt tatccggaag ttgatgatga
    31921 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    31981 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    32041 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aataaagacc aggccatgaa
    32101 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgctg agaaacaggc
    32161 gatggaagat aacaaatccg acatcggctg gggcagccag attcgttctt atgtccttga
    32221 tgactcccgc attaaagatc tgcgcaccgg ggttgaaacc cgcaatacgc aggccgtgct
    32281 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    32341 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    32401 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    32461 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    32521 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    32581 gggtaaagcc tcttttgtta ccctgcagga cgttggcggt cgtattcagc tgtacgttgc
    32641 ccgtgacgat cttccggaag gcgtttacaa cgagcagttc aaaaaatggg atctcggcga
    32701 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    32761 caccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggacaaat tccacggctt
    32821 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    32881 ccgcaacacc tttaaagtgc gttcgcagat cctctctggc attcgccagt tcatggtaaa
    32941 tcgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    33001 gcgtccgttt atcacccatc ataacgcgct ggatcttgac atgtacctgc gtatcgcgcc
    33061 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    33121 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    33181 ctatatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    33241 tctggcacag gatattctcg gtaagacgga agtcacctac ggcgacgtga cgctggactt
    33301 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    33361 cgacatggcg gatctggaca acttcgactc tgcaaaagcg attgctgaat ctatcggcat
    33421 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    33481 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    33541 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    33601 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcagcgctt
    33661 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgacgaaga
    33721 ttacgtcacc gcactggaac acggcttacc gccgacagca ggtctgggaa ttggtatcga
    33781 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    33841 gatgcgtcca gtaaaataag cattacgtta tgctaataac cccggcaaat gtcggggttt
    33901 tttaattgtg ctgcgcgaaa gcagacaaca attttcttgc ttcactattg gctgcctgca
    33961 ttaccatcca cggactgaac gcccacggcg tggcatcaat accgtgtaat acatctgcta
    34021 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctgttggtgc
    34081 gtgcggcaaa taccggacac acttcatttt ccacaatgcc attcggatcg gtggcgcgat
    34141 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    34201 aacggcggat cactgcgtct tcgttgcttt ctcccagttg tgggtgccca caaaccgagt
    34261 tagtccacac gccaggccat gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    34321 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    34381 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    34441 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    34501 tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    34561 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    34621 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacagcagac tgaatatagc
    34681 gcgcattaaa agatttatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    34741 gccacatacg tactgtcctg acttttaaat ctttatcaga gacggattcc ttcaccaggt
    34801 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    34861 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    34921 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    34981 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    35041 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    35101 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    35161 tatgaacgcg cgtcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    35221 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg cgaataatat
    35281 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    35341 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    35401 gcataccaaa cgacatcggg gtgacaatcg caaaccacaa ggcatcatgt agcccggata
    35461 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    35521 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    35581 caaaggagat acctgaataa acgggattac catattgcgg attcccttta cctccggcgg
    35641 cccagtcaat acccacctga atgatgctaa gtccgacaga agtaataacc acaccggtaa
    35701 ccagtggcgg gaataaaggc atcaagcgac cgataagtgg cgctaataat gtggtgataa
    35761 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc aatatccggg ttcatcccaa
    35821 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    35881 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    35941 ccgagctaat gagcatcgca atagcttctt ttgagaggcc cagtcggtca ccaatcataa
    36001 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    36061 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    36121 agagaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagagtaaaa
    36181 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    36241 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg gttaaaccac
    36301 gaaaatgaga agagaacctg acaatggaat tatctgtaat tttctcatat tgatatttca
    36361 ttaatcattc tggcatgtcg tactctcgca tttaatcgtt tttatctgga tagcactctt
    36421 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    36481 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt t
//
LOCUS       DAJTSL010000021        36502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTSL010000021 DAJTSL010000000
VERSION     DAJTSL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.021"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            260..346
                     /gene="leuL"
                     /locus_tag="OO433_000928"
     CDS             260..346
                     /gene="leuL"
                     /locus_tag="OO433_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6049494.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            439..2010
                     /gene="leuA"
                     /locus_tag="OO433_000929"
     CDS             439..2010
                     /gene="leuA"
                     /locus_tag="OO433_000929"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6049495.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2010..3101
                     /gene="leuB"
                     /locus_tag="OO433_000930"
     CDS             2010..3101
                     /gene="leuB"
                     /locus_tag="OO433_000930"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6049496.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3104..4504
                     /gene="leuC"
                     /locus_tag="OO433_000931"
     CDS             3104..4504
                     /gene="leuC"
                     /locus_tag="OO433_000931"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6049497.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGQVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4515..5120
                     /gene="leuD"
                     /locus_tag="OO433_000932"
     CDS             4515..5120
                     /gene="leuD"
                     /locus_tag="OO433_000932"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6049498.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPVIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5169..6251)
                     /locus_tag="OO433_000933"
     CDS             complement(5169..6251)
                     /locus_tag="OO433_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6049499.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYEDNSAREVVMFSIAAGIFTLSLVLIAFML
                     PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPD
                     KLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALM
                     TLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSW
                     GHFAVYWVIAVISVVALFLTAKVKDV"
     gene            complement(6352..6483)
                     /gene="sgrT"
                     /locus_tag="OO433_000934"
     CDS             complement(6352..6483)
                     /gene="sgrT"
                     /locus_tag="OO433_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6049500.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6572..8227
                     /gene="sgrR"
                     /locus_tag="OO433_000935"
     CDS             6572..8227
                     /gene="sgrR"
                     /locus_tag="OO433_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6049501.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSTLRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVNYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8261..8360
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8391..9374
                     /gene="thiB"
                     /locus_tag="OO433_000936"
     CDS             8391..9374
                     /gene="thiB"
                     /locus_tag="OO433_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6049502.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISGWQRAVSR"
     gene            9350..10960
                     /gene="thiP"
                     /locus_tag="OO433_000937"
     CDS             9350..10960
                     /gene="thiP"
                     /locus_tag="OO433_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6049503.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPDEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLQQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            10944..11642
                     /gene="thiQ"
                     /locus_tag="OO433_000938"
     CDS             10944..11642
                     /gene="thiQ"
                     /locus_tag="OO433_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6049504.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11755..12519)
                     /locus_tag="OO433_000939"
     CDS             complement(11755..12519)
                     /locus_tag="OO433_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6049505.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12605..13483)
                     /gene="araC"
                     /locus_tag="OO433_000940"
     CDS             complement(12605..13483)
                     /gene="araC"
                     /locus_tag="OO433_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6049506.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13822..15522
                     /gene="araB"
                     /locus_tag="OO433_000941"
     CDS             13822..15522
                     /gene="araB"
                     /locus_tag="OO433_000941"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6049507.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQTQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15533..17035
                     /gene="araA"
                     /locus_tag="OO433_000942"
     CDS             15533..17035
                     /gene="araA"
                     /locus_tag="OO433_000942"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6049508.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQHLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17235..17930
                     /gene="araD"
                     /locus_tag="OO433_000943"
     CDS             17235..17930
                     /gene="araD"
                     /locus_tag="OO433_000943"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6049509.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18005..20356
                     /gene="polB"
                     /locus_tag="OO433_000944"
     CDS             18005..20356
                     /gene="polB"
                     /locus_tag="OO433_000944"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6049510.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGSENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20520..23426
                     /gene="rapA"
                     /locus_tag="OO433_000945"
     CDS             20520..23426
                     /gene="rapA"
                     /locus_tag="OO433_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6049511.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHMPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLLDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23438..24097
                     /gene="rluA"
                     /locus_tag="OO433_000946"
     CDS             23438..24097
                     /gene="rluA"
                     /locus_tag="OO433_000946"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6049512.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24214..25029)
                     /gene="djlA"
                     /locus_tag="OO433_000947"
     CDS             complement(24214..25029)
                     /gene="djlA"
                     /locus_tag="OO433_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6049513.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25284..27638
                     /gene="lptD"
                     /locus_tag="OO433_000948"
     CDS             25284..27638
                     /gene="lptD"
                     /locus_tag="OO433_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6049514.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVEHFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27691..28977
                     /gene="surA"
                     /locus_tag="OO433_000949"
     CDS             27691..28977
                     /gene="surA"
                     /locus_tag="OO433_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6049515.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            28977..29966
                     /gene="pdxA"
                     /locus_tag="OO433_000950"
     CDS             28977..29966
                     /gene="pdxA"
                     /locus_tag="OO433_000950"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6049516.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMILNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            29963..30784
                     /gene="rsmA"
                     /locus_tag="OO433_000951"
     CDS             29963..30784
                     /gene="rsmA"
                     /locus_tag="OO433_000951"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6049517.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30787..31164
                     /gene="apaG"
                     /locus_tag="OO433_000952"
     CDS             30787..31164
                     /gene="apaG"
                     /locus_tag="OO433_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6049518.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31171..32013
                     /gene="apaH"
                     /locus_tag="OO433_000953"
     CDS             31171..32013
                     /gene="apaH"
                     /locus_tag="OO433_000953"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6049519.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32091..32570)
                     /gene="folA"
                     /locus_tag="OO433_000954"
     CDS             complement(32091..32570)
                     /gene="folA"
                     /locus_tag="OO433_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6049520.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32762..34624)
                     /gene="kefC"
                     /locus_tag="OO433_000955"
     CDS             complement(32762..34624)
                     /gene="kefC"
                     /locus_tag="OO433_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6049521.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34617..35147)
                     /gene="kefF"
                     /locus_tag="OO433_000956"
     CDS             complement(34617..35147)
                     /gene="kefF"
                     /locus_tag="OO433_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6049522.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35255..36502)
                     /locus_tag="OO433_000957"
                     /pseudo
     CDS             complement(35255..36502)
                     /locus_tag="OO433_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8816 a        10036 c         9648 g         8002 t
ORIGIN      
        1 gttttgcaac gcgtgcattt tgtccccttt ttcctcgttg attagatgca aaaatttatt
       61 ctgaaatatg tcaacctatg aaaagtgtcg gtcgttaaac agaaattaat atcgcttgct
      121 ttaaccaccg cagcacaatt agctaatttt acggatgcag aactcgcgct ggcgggacgt
      181 ttttattgcg tcagggttga catccgtttt tgtatccagt aactctaaaa gcatatcgca
      241 ttcatctgga gctgatttaa tgactcacat cgttcgcttt atcggtctac tactactaaa
      301 cgcatcttct ttgcgcggta gacgagtgag cggcatccag cattaagcca gcacgcagtc
      361 aaacaaaaaa cccgcgccat tgcgcgggtt tttttatgcc cgaagcgagg cgctctaaaa
      421 gagacaagga cccaaaccat gagccagcaa gtcattattt tcgataccac attgcgcgac
      481 ggtgaacagg cgttacaggc aagcttgagt gtgaaagaaa aactgcaaat tgcgctggcc
      541 cttgagcgta tgggtgttga cgtgatggaa gtcggtttcc ccgtctcttc gccgggcgat
      601 tttgaatcag tgcaaaccat cgcccgccag gttaaaaaca gccgcgtatg cgcgttagct
      661 cgctgcgtgg agaaagatat cgacgtggcg gccgaatccc tgaaagtcgc cgaagccttc
      721 cgtattcata cctttattgc cacttcgcca atgcacatcg ccaccaagct gcgcagcacg
      781 ctggatgaag tgatcgaacg cgctatctat atggtgaaac gcgcccgtaa ttacaccgat
      841 gatgttgaat tttcttgcga agatgccggg cgtaccccca ttgccgatct ggcgcgagtg
      901 gtcgaagcgg cgattaatgc cggtgccacc accatcaaca ttccggacac cgtgggctac
      961 accatgccgt ttgagttcgc cggaatcatc agcggtctgt atgaacgcgt acctaacatc
     1021 gacaaagcca ttatttccgt acatacccac gacgatttag gcctggcggt cggcaactca
     1081 ctggcggcgg tacatgccgg tgcacgccag gtggaaggtg caatgaacgg gatcggcgag
     1141 cgtgccggta actgttcgct ggaagaagtc atcatggcga ttaaagttcg taaggatatt
     1201 ctcaacgtcc acaccgccat taatcaccag gagatatggc gcaccagcca gttagttagc
     1261 cagatttgta atatgccgat cccggcaaac aaagccattg ttggcagcgg cgcattcgcc
     1321 cactcctccg gtatccacca ggatggcgtg ctgaaaaacc gcgaaaacta cgaaatcatg
     1381 acaccagaat ctattggtct gaaccaaatc cagctgaatc tgacctctcg ttcggggcgt
     1441 gcggcggtga aacatcgcat ggatgagatg gggtataaag aaagtgaata taatttagac
     1501 aatttgtacg atgctttcct gaagctggcg gacaaaaaag gtcaggtgtt tgattacgat
     1561 ctggaggcgc tggccttcat cggtaagcag caagaagagc cggagcattt ccgtctggat
     1621 tacttcagcg tgcagtctgg ctctaacgat atcgccaccg ccgccgtcaa actggcctgt
     1681 ggcgaagaag tcaaagcaga agccgccaac ggtaacggtc cggtcgatgc cgtctatcag
     1741 gcaattaacc gcatcactga ctataacgtc gaactggtga aatacagcct gaccgccaaa
     1801 ggtcacggta aagatgcgct gggtcaggtg gatattgtcg ccaactacaa cggtcgccgc
     1861 ttccacggcg tcggcctggc caccgatatt gtcgagtcct ccgccaaagc catggtgcac
     1921 gtactgaaca atatctggcg tgccgcagaa gtcgaaaaag agttgcaacg caaagctcaa
     1981 cacaacgaaa acaacaagga aaccgtgtga tgtcgaagaa ttaccatatt gccgtattgc
     2041 cgggggacgg tattggtccg gaagtgatga cccaggcgct gaaagtgctg gatgccgtgc
     2101 gcaaccgctt tgcgatgcgc atcaccacca gccattacga tgtaggcggc gcagccattg
     2161 ataaccacgg gcaaccactg ccgcctgcga cggttgaagg ttgtgagcaa gccgatgccg
     2221 tgctgtttgg ctcggtaggc ggtccgaaat gggaacattt accaccagac cagcaaccag
     2281 aacgcggcgc gctgttgcct ttgcgtaagc acttcaaatt attcagcaac ctgcgtccgg
     2341 caaaactgta tcaggggctg gaagcattct gtccgctgcg tgctgacatt gccgctaacg
     2401 gcttcgacat cctgtgcgtg cgcgaactga ccggcggcat ctatttcggt cagccaaaag
     2461 gccgcgaagg tagcggacag tatgaaaaag cgtttgatac cgaggtgtat caccgttttg
     2521 agatcgaacg tatcgcccgc atcgcgtttg aatctgcccg caagcgtcgc cacaaagtca
     2581 cctcaatcga caaagccaac gtgctgcaat cctctatttt atggcgggag atcgttaacg
     2641 agatcgccac ggaatacccg gatgtcgaac tggcgcatat gtacatcgac aacgccacca
     2701 tgcagctgat taaagatcca tcacagtttg acgtcctgct gtgctccaac ctgtttggcg
     2761 acattctgtc tgacgagtgc gcaatgatca ctggctcgat ggggatgttg ccttccgcca
     2821 gcctgaacga gcaaggtttt ggtctgtatg aaccggcagg cggctcagca ccagatatca
     2881 caggcaaaaa catcgccaac ccgattgcgc aaattctgtc gctggcactg ctgctgcgct
     2941 acagcctgga tgccgatgat gcggcttgcg ccattgaacg cgccattaac cgcgcattag
     3001 aagaaggcat tcgcaccggg gatttagccc gtggcgctgc cgccgttagt accgatgaaa
     3061 tgggcgatat cattgcccgc tatgtggcag aaggggtgta atcatggcta aaacgttata
     3121 cgaaaaatta ttcgacgctc acgtagtgta cgaagccgaa aacgaaaccc cgctgttata
     3181 tatcgaccgc cacctggtgc atgaagtgac ctcaccgcag gcgttcgatg gtctgcgcgc
     3241 ccacggtcgc ccggtacgtc agccgggcaa aaccttcgcc actatggatc acaacgtctc
     3301 tacccagacg aaagacatta atgcctgtgg tgaaatggcg cgcatccaga tgcaggagct
     3361 gattaaaaac tgcaaagaat ttggcgtcga actgtatgac ctgaatcacc cgtatcaggg
     3421 gatcgtccac gtaatggggc cggaacaggg cgtcaccttg ccggggatga ccattgtctg
     3481 cggcgactcg cataccgcca cccacggcgc gtttggcgca ctggcctttg gtatcggcac
     3541 ttccgaagtt gaacacgtac tggcaacgca aaccctgaaa cagggccgcg caaaaaccat
     3601 gaaaattgaa gtccagggca aagccgcgcc gggcattacc gcaaaagata tcgtgctggc
     3661 aattatcggt aaaaccggta gcgcaggcgg caccgggcat gtggtggagt tttgcggcga
     3721 agcaatccgt gatttaagca tggaaggtcg tatgaccctg tgcaatatgg caatcgaaat
     3781 gggcgcaaaa gccggtctgg ttgcaccgga cgaaaccacc tttaactatg tcaaaggccg
     3841 tttgcatgcg ccgaaaggca aagatttcga cgacgccgtt gcctactgga aaaccctgca
     3901 aaccgacgaa ggcgcaactt tcgataccgt tgtcactctg caagcagaag aaatttcacc
     3961 gcaggtcacc tggggcacca atccaggcca ggtgatttcc gtgaacgaca atattcccga
     4021 tccggcttcg tttgccgatc cggttgaacg cgcgtcggca gaaaaagcgc tggcctatat
     4081 ggggctgaaa ccgggcattc cgctgaccga agtggctatc gacaaagtgt ttatcggttc
     4141 ctgtaccaac tcgcgcattg aagatttacg cgcggcagcg gagatcgcca aagggcgaaa
     4201 agtcgcgcca ggcgtgcagg cactggtggt tcccggctct ggccaggtaa aagcccaggc
     4261 ggaagcggaa ggtctggata aaatctttat tgaagccggt tttgaatggc gcttgcctgg
     4321 ctgctcaatg tgtctggcga tgaacaacga ccgtctgaat ccgggcgaac gttgtgcatc
     4381 caccagcaac cgtaactttg aaggccgcca ggggcgcggc gggcgcacgc atctggtcag
     4441 cccggcaatg gctgccgctg ctgctgtgac cggacatttc gccgacattc gcaacattaa
     4501 ataaggagca gaccatggca gagaaattta tcaaacacac aggcctggtg gttccgctgg
     4561 atgccgccaa tgtcgatacc gatgcaatca tcccgaaaca gtttttgcag aaagtgaccc
     4621 gtacgggttt tggcgcgcat ctgtttaacg actggcgttt tctcgatgaa aaaggccaac
     4681 agccaaaccc ggacttcgtg ctgaacttcc cgcagtatca gggcgcttcc attttgctgg
     4741 cacgagaaaa cttcggctgt ggctcctcgc gtgagcacgc gccctgggca ttgaccgact
     4801 acggttttaa agtggtgatt gcgccgagtt ttgctgacat cttctacggc aatagcttta
     4861 acaaccagct gctgccggtg aaattaagcg atgcagaagt ggacgaactg tttgcgctgg
     4921 tgaaagccaa tccggtgatc catttcgacg tggatctgga agcgcaagag gtgaaagcgg
     4981 gagagaaaac ctatcgcttt accatcgatg ccttccgccg ccactgcatg atgaacggtc
     5041 tggacagtat tgggcttacc ttgcagcacg acgacgccat tgccgcttat gaagcaaaac
     5101 aacctgcgtt tatgaattaa tccccttgcc cggtcaaatg accgggcttt ccgctatcgt
     5161 ccacgtcatc aaacgtcttt aacctttgcg gttaaaaata atgcgacaac agaaataacc
     5221 gcaattaccc agtagacagc aaagtgcccc caactttgtg caattgctcc ctgaataacg
     5281 ccagccagaa ttaccccggt agaaatactg ttagtaaata aggtggtagc tgcccccgct
     5341 cttccaggca ttaaatcctg aaaccatagc atcccaatac ccgcaacaat gccgataaat
     5401 acagcgttaa aaagttgcag cgtcatcaac gccatacggc tatgaaagaa aatcaatccg
     5461 gtgtaaaaca gtactcctgc cgccactgct atgaccatca ttcgccgctt accataacgt
     5521 ttgacatagt agccagccag aatcattgct ggtatttcca gtccagctgc cgtccccatc
     5581 aggaaacccg ccagtttgtc tggcaatcct aactcgctac tgatccacaa cggcatatca
     5641 ataatgtaca tggtgttgca ggtccacatt aacgtcgagg cgacaaataa catccgtacg
     5701 ttactatcct gccagccacc ttgcattgat aaagcatttt ccgacggcag ttctacccgc
     5761 gccacagacg gaagcataaa tgcaatcaat accagactga gtgtgaatat cccggcggca
     5821 atcgaaaaca tcaccacttc tcgcgccgag ttatcctcat attcccgcgc cagagcaaat
     5881 aactgtggca ttgccgtatt ggccagagat gccagaagca caccacaggt gataagcgtc
     5941 agataatgac gattaaatgc aaacaatggc gcattgccga tagccatcaa acagcaaaat
     6001 ataatcagtt ttcgccgatc gccctgactg tcagaacgtt ttgccaacca gaggcttacg
     6061 ccgatcccag caatagtatt caccgtataa aagagtccga tccagaaagg ttgcgcgcca
     6121 acctcacgac tcagaaataa gctcaatgta ggagcctgta gcgccccggc cacccccatc
     6181 ataaaagcga ccagcataaa tgccgcgtaa acaccgttca tacggcgagc catcgtcatt
     6241 atccagatca tacgttccct ttttagcgcg gcgagaataa aaaaaccagc aggtataatc
     6301 tgctggcggg tgattttaca ccaatactca gtcacacatg atgcaggcaa gtcaactttc
     6361 agaattgcgg tcatcccact gcatcagtcc ttcaagcatg gttaatcgtt gtgggacgct
     6421 taaccaacgc aaccagcaca acttcgctgt cgcggtaaaa tagtgctgat aaaactgacg
     6481 catggggcac ctccttgctt catcgttagg aattattgac ttaatatagg gaaaataaaa
     6541 ttgctgtctt ttgcgcagga gttcctcttt tatgccatct gctcgtctgc aacaacagtt
     6601 catccgcctg tggcaatgct gcgagggtaa atcgcaggac acaacgctca acgaactggc
     6661 agcgttattg agctgctcgc gtcgtcatat gcgtaccctg ctcaacacca tgcaggatcg
     6721 cggctggctg acgtgggaag cggaagtcgg gcgcggtaaa cgctcgcgtc tgacattcct
     6781 ctataccggg ctggcgcttc agcaacagcg ggcggaagac ctgctggagc aggatcgtat
     6841 cgaccaacta gtgcagttgg ttggcgacaa agcgactgtg cggcaaatgc tggtttctca
     6901 tctgggccgc agcttccgcc aggggcggca catcctgcgc gtgctctact atcgtccgtt
     6961 gcgtaatctg ctacctggca gcacattgcg ccgttccgaa acccatattg cccggcaaat
     7021 cttcagttcg ctaacgcgca taaatgagga aaatggggaa ctggaagcag acatcgccca
     7081 ccactggcag caaatatcac cgcttcactg gcgtttcttt ttgcgtccag gagtccattt
     7141 tcatcatggt cgtgaactgg aaatggacga cgtgatcgcc tctttaaaac gaatcaatac
     7201 gctgccgctc tattcgcata ttgcggacat tgtgtcgccg acgccctgga cgctggatat
     7261 ccatctcacg caaccggacc gctggttacc gttactgctg gggcaagttc cggcgatgat
     7321 cctgccgcgc gaatgggaaa ccctcagtaa ctttgccagc catcccatcg gcaccggtcc
     7381 gtatgcggtg atccgcaaca ccaccaatca actgaaaatt caggcattcg atgacttctt
     7441 cggttaccgg gcattaatcg atgaagttaa cgtctgggtt ctgccggaaa ttgccgacga
     7501 gccagctgga ggtctgatgc taaaaggtcc acagggtgag gaaaaagaga ttgaaagccg
     7561 cctggaggaa ggttgctact atttactatt cgacagccgc acccatcgcg gagcgaatca
     7621 gcaagtcagg gactgggtaa actatgtgct ttctccaact aatctggtct atttcgctga
     7681 ggaacagtac cagcaactgt ggttcccggc ttatggactg ctcccccgtt ggcaccatgc
     7741 ccgcaccata aagagcgaaa aaccggctgg cctggaaagc ctcaccctga ccttttatca
     7801 ggatcacagt gagcatcggg tgattgccgg gatcatgcag cagattctgg caagtcacca
     7861 ggtcacgctg gaaatcaaag agatcagcta cgatcagtgg catgaaggag agatcgagag
     7921 cgatatctgg cttaatagcg ccaactttac gctgccgctg gatttttcgc tgttcgcgca
     7981 cttgtgcgag gtgccgctgc tccaacactg tattccgatc gactggcaag ccgacgccgc
     8041 ccgctggcgc aatggcgaaa tgaacctggc gaactggtgc cagcaactgg tcgccagtaa
     8101 agcaatggtg ccgcttatcc accactggct gatcattcag ggacaacgca gtatgcgcgg
     8161 cctgcgtatg aataccctcg gctggttcga ttttaaatca gcgtggtttg cgccgccgga
     8221 tccatgattg ctggtagata acaaaatcac tacactaacg ccgttctcaa cggggtgcca
     8281 cgcaaacgcg tgcgctgaga aaatacccgt cgaacctgat ccggataacg ccggcgaagg
     8341 gatttgaggc tccttctcaa gtcctttgcc actctttttt gaggtgcaaa gtgttaaaaa
     8401 aatgtcttcc cctgctgttg ctgtgcacag cgcccgtttt cgctaaaccc gttctgactg
     8461 tttataccta cgattccttc gccgccgact gggggcctgg tccaaagatc aaaaaagctt
     8521 ttgaagccga ctgtaactgc gaactgaaac tggtggcgct ggaagatggc gtttcgcttc
     8581 tcaaccgtct acggatggaa ggcaaaaata gtaaagccga tgtggtgctg gggctggata
     8641 acaatctgtt agacgccgcc agtaaaaccg gactatttgc caaaagcggt gtggcagcgg
     8701 atgccgttaa cgttcccggc ggctggaata atgacacttt cgtcccgttt gattacggct
     8761 atttcgcctt cgtctatgac aagaacaaac tgaaaaaccc gccgcaaagc ctgaaagaat
     8821 tggttgagag cgatcaaaac tggcgggtga tttatcagga tccgcgcacc agtacgccgg
     8881 ggctgggcct gctgttgtgg atgcaaaaag tctatggcga tgacgcccca caagcctggc
     8941 aaaaactggc gaagaaaacc gtcacggtca ccaaaggctg gagcgaagcc tacggcctgt
     9001 ttttaaaagg tgaaagcgat ctggtactga gttacaccac ctctccggct tatcacattc
     9061 tcgaagagaa aaaagataac tacgccgccg cgaactttag cgaaggtcac tatctgcagg
     9121 tggaagtcgc cgcccgcacc gctgccagca agcagccgga gctggcgcaa aaattcctcc
     9181 agtttatggt ttctccggct tttcagaatg cgatcccaac cggtaactgg atgtatccgg
     9241 tggcaaatgt cacgctgcct gccgggtttg aacaattgac caaaccggcc accacgctgg
     9301 agttcacacc agctgaagtg gcggcacaac gtcaggcatg gattagcgga tggcaacgcg
     9361 ccgtcagccg ttaattcccg gctggttaat tccaggtgta agcgccgcca cgctggtggt
     9421 agcggttgcg ctcgcggcgt ttctcgccct gtggtggaac gcgccgcagg gtaactgggt
     9481 ggcagtctgg caggacagct acctgtggca tgtggtgcgc ttctccttct ggcaggcgtt
     9541 tctctcggcg ctactctctg tcgtacccgc gatattcctc gcccgcgccc tctatcgcag
     9601 gcgctttccg ggtcggctgg cgctgttgcg tctgtgcgca atgaccttga tcctcccggt
     9661 gctggttgct gttttcggca ttcttagcgt ctatggtcgc cagggctggc tggcatcgct
     9721 ctgccaatcg ctcggtctgg agtggacctt ttcgccctac ggcctgcaag gtattttgct
     9781 ggcgcacgta ttttttaatc tgccgatggc gagccgctta ttactccagg cactggaaaa
     9841 cattcccgac gaacaacgtc agcttgccgc ccagcttggg atgcgcggct ggcatttttt
     9901 ccgcttcgtc gaatggccgt ggttacggcg acaaatcccg ccggttgctg cacttatctt
     9961 tatgctctgt ttcgccagct tcgccaccgt gctatcactg ggcggcggtc cgcaggcgac
    10021 cactatcgag ctggctattt atcaggcgct gagttacgac tacgatcctg cccgcgcggc
    10081 gatgctggcg ctgatccaga tggtgtgttg ccttgggctg gtactgttga gtcagcgatt
    10141 gagtaaggcc attgcgccag gcaccacgct gctgcaaggc tggcgcgacc cggacgatcg
    10201 tctgcatagc cgcatttgcg acacggtgtt aatagtgctg gcgttgctgc tgttgttgcc
    10261 accgttgctg gcggtgatcg tcgatggggt aaatcgccag ttgccggaag tgctggcaca
    10321 accggtgctg tggcaggcgc tgtggacctc gttgcgtatt gcgctggcgg caggtgtatt
    10381 gtgcgtagtg ctgaccatga tgctgctatg gagcagtcgc gaacttcggg cacggcagaa
    10441 aatgctggcg ggtcaggcgc tggagatgag cggcatgttg attctcgcca tgccggggat
    10501 tgtgctggct accggcttct ttttactgct caacaacact atcggcctgc aacaatctgc
    10561 tgacggcatt gtgattttca ccaatgcgtt aatggcgatc ccttatgcgc tgaaagtact
    10621 ggaaaacccg atgcgcgata tcaccgcccg ctacagtatg ttgtgtcagt cgctgggcat
    10681 tgaaggctgg tcgcgcttaa aagtggtgga gctgcgcgcc ctgaaacgtc cactggcgca
    10741 ggcgctggcc tttgcctgcg tgctgtcgat tggtgatttt ggcgtggtgg cgttgttcgg
    10801 taacgatgat ttccgtaccc tgccgtttta tctctaccag caaattggct cctatcgcag
    10861 ccaggacggc gcggtcaccg cgttaattct gctgctactc tgttttctgc tgtttaccgt
    10921 gattgaaaaa ctaccggggc gaaatgttaa aactgactga tatcacctgg ctttaccaac
    10981 atttgccgat gcgttttagc ttaacggtgg aacgcggcga gcaggtggcg atcctcgggc
    11041 caagcggcgc aggtaaaagt accctgctga atttgattgc cggttttctg acgccagcca
    11101 gcggttcgct gactatcgat ggcgttgatc acaccacgac gccgccgtca cgccgtccgg
    11161 tgtcgatgct gtttcaggag aacaacctgt tcagccacct gacggtcgcg cagaacatcg
    11221 ggctggggct taatccagga ttgaagctga acgcggcaca gcaggagaaa atgcacgcta
    11281 tcgcccgtca gatggggatt gataatttaa tggcgcggtt accgggcgag ctttccggcg
    11341 gccagcgaca gcgcgtggcg ttagcgcgtt gtctggtgcg cgaacagccg attttattgc
    11401 tcgatgaacc gttctctgcg ctcgatccgg cgttacgtca ggagatgctg acgctggtga
    11461 gcacgagctg ccagcagcaa aaaatgacgc tattgatggt gtcgcacagt gtggaagatg
    11521 cggcgcggat cgccacacgc tcggtggtgg tcgccgacgg gcgcatcgcc tggcagggta
    11581 agactgaaga attgttgagc ggtaaggcga gtgcttcggc aatattgggg attacgggtt
    11641 agtatgcact gtcagatgcg gcgtgaacac cttatccgac ctacggacag tgcgttttgt
    11701 aggcctgata agaccggtca gcgtcgcatc aggcaacccg cacaagacaa tcccctaaac
    11761 cccaaccact ttacgcaaaa tatcgatata caccggcatc aacgggtggc gaattaacac
    11821 caccagcgcc accacgccga tggcagaaat caacggcgtc agccacaaca aacggccgcg
    11881 cgacaaataa tgacttaaac gatcggttgc tttaccgctg cgccataacc gccagcacag
    11941 ccagccacca acccacaaaa ataccgccgt cgccagcagc aaccatttaa actcgccgct
    12001 ctgcattccg gctggaatat cgatcgccgc gcccgccaga atcccaggca ggaagtaaaa
    12061 cggtggccac agcaggcagc cgataatatt cggcgtaatg aattttgcca ccggcagatc
    12121 cagcattccc gccaccattg gcaccagcgg acgcgtcggg ccaacaaagc gaccgaccag
    12181 aatggtgaac atgctgtgtt gatgcaacgc atgttcagtt ttatcgagta atgctttgtt
    12241 tttcttcaga aatgaccagc gatgcaacgg ctttttaaaa cgccagccca gccagaagga
    12301 gatccagtcg cccagcagac aaccgacaat ccctgccagc caggcgtgcc agaaacttaa
    12361 ctcgccgctg ccaatcagcg ctcccagccc cgccatcagc accgtaccgg gtagaatcaa
    12421 accgaccagc gccagcgact ccagaaaggc caccaacacc accgccatca atgaatacac
    12481 ggtagattgg gtaataaagt gttccagcaa tgcttgcata atgtgcctgt caaatggacg
    12541 aagcagggat tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta
    12601 ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact
    12661 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa
    12721 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg
    12781 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga
    12841 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa
    12901 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca
    12961 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca
    13021 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc
    13081 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaaa cccgtattgg
    13141 caaatattga cggccagtta agccattcat gccaataggc gcgcggacga aagtaaaccc
    13201 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg
    13261 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc
    13321 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa
    13381 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa
    13441 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc
    13501 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt
    13561 actggctctt ctcgctaacc caaccggtaa ccccgcttat taaaagcatt ctgtaacaaa
    13621 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag
    13681 tccacattga ttatttgcac ggcgtcacac tttgctattc catagcattt ttatccataa
    13741 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt
    13801 ttttttggat ggagtgaaac gatggcgatt gcaattggcc tcgattttgg cagtgattct
    13861 gtgcgagctt tggcggtgga ctgcgccagc ggtgaagaga tcgccaccag cgtagagtgg
    13921 tatccccgtt ggcaaaaagg gcaattttgt gatgccccga ataaccagtt ccgtcatcat
    13981 ccgcgtgact acattgagtc aatggaagcg gcactgaaaa ccgtgcttgc agagcttagc
    14041 gtcgaacagc gcgcagctgt ggtcgggatt ggcgttgaca gtaccggctc gacgcccgca
    14101 ccgattgatg ccgacggtaa cgtgctggcg ctgcgcccgg agtttgccga aaacccaaac
    14161 gcgatgttcg tattgtggaa agaccacact gcggttgaag aagcggaaga gattacccgt
    14221 ttgtgccacg cgccgggcaa cgttgactac tcccgctaca ttggtggtat ttattccagc
    14281 gaatggttct gggcaaaaat cctgcatgtg actcgccagg acagcgccgt ggcgcaatct
    14341 gccgcatcgt ggattgagct gtgcgactgg gtgccagctc tgctttccgg taccacccgc
    14401 ccgcaggata ttcgtcgcgg acgttgcagc gccgggcata aatctctgtg gcacgaaagc
    14461 tggggcggct tgccgccagc cagtttcttt gatgagctgg acccgatcct caatcgccat
    14521 ttgccttccc cgctgttcac tgacacctgg actgccgata ttccggtggg caccttatgc
    14581 ccggaatggg cgcagcgtct cggcctgcct gaaagcgtgg tgatttccgg cggcgcgttt
    14641 gactgccata tgggcgcagt tggcgcaggc gcacagccta acgcactggt aaaagttatc
    14701 ggtacttcca cctgcgacat tctgattgcc gacaaacaga gcgttggcga gcgggcagtt
    14761 aaaggtattt gcggtcaggt tgatggcagc gtggtacctg gatttatcgg tctggaagca
    14821 ggccaatcgg cgtttggtga tatctacgcc tggtttggtc gcgtactcgg ctggccgctg
    14881 gaacagcttg ccgcccagca tccggaactg aaagcgcaaa tcaacgccag ccagaaacaa
    14941 ctgcttccgg cgctgaccga agcatgggcc aaaaatccgt ctctggatca cctgccggtg
    15001 gtgctcgact ggtttaacgg ccgccgcaca ccgaacgcta accaacgcct gaaaggggtg
    15061 attaccgatc ttaacctcgc taccgacgct ccgctgctgt ttggcggttt gattgctgcc
    15121 accgcctttg gcgcacgcgc aatcatggag tgctttaccg atcaggggat cgccgtcaat
    15181 aacgtgatgg cgctgggcgg catcgcgcgg aaaaaccagg tcattatgca ggcctgctgc
    15241 gacgtgctga atcgcccgct gcaaattgtt gcctctgacc agtgctgtgc gctcggtgcg
    15301 gcgatttttg ctgccgtcgc cgcgaaagtg cacgcagaca tcccatcagc ccagcaaaaa
    15361 atggccagtg cggtagagaa aaccctgcaa ccgcgcagcg aacagacaca acgctttgaa
    15421 cagctttatc gccgctatca gcaatgggcg atgagcgccg aacaacacta tcttccaact
    15481 tccgccccgg cacaggctgc ccaggccgtt ccgactctat aaggacacga taatgacgat
    15541 ttttgataat tatgaagtgt ggtttgtcat tggcagccag catctgtatg gcccggaaac
    15601 cctgcgtcag gtcacccaac atgccgagca cgttgttaat gcgctgaata cggaagcgaa
    15661 actgccctgc aaactggtgt tgaaaccgct gggcaccacg ccggatgaaa tcaccgctat
    15721 ttgccgcgac gcgaattacg acgatcgttg cgctggtctg gtggtgtggc tgcacacctt
    15781 ctccccggcc aaaatgtgga tcaacggcct gaccatgctc aacaaaccgt tgctgcaatt
    15841 ccacacccag tttaacgcgg cgctgccgtg ggacagtatc gatatggact ttatgaacct
    15901 gaaccagact gcacatggcg gtcgcgagtt cggcttcatt ggcgcgcgta tgcgtcagca
    15961 acatgccgtg gttaccggtc actggcagga taaacaagcc catgagcgta tcggctcctg
    16021 gatgcgtcag gcggtctcta aacaggatac ccgtcatctg aaagtctgcc gttttggcga
    16081 taacatgcgt gaagtagcgg tcaccgatgg tgataaagtt gccgcacaga tcaagttcgg
    16141 tttctccgtc aatacctggg cggttggcga tctggtgcag gtggtgaact ccatcagcga
    16201 cggcgatgtt aacgcgctgg tcgatgagta cgaaagctgc tacaccatga cgcctgccac
    16261 acaaatccac ggcgaaaaac gacagaacgt gctggaagcg gcgcgtattg agctggggat
    16321 gaagcgtttc ctggaacaag gtggcttcca cgcgttcacc accacctttg aagatttgca
    16381 cggtctgaaa cagcttccag gtctggccgt acagcatctg atgcagcagg gttacggctt
    16441 tgcgggcgaa ggcgactgga aaaccgccgc cctgcttcgc atcatgaagg tgatgtcaac
    16501 cggtctgcag ggcggcacct cctttatgga ggactacacc tatcacttcg agaaaggtaa
    16561 tgacctggtg ctcggctccc atatgctgga agtctgcccg tcgattgccg tagaagagaa
    16621 accgatcctc gacgttcagc atctcggtat tggtggtaag gacgatcctg cccgactgat
    16681 cttcaatacc caaaccggtc cagcgattgt cgccagcctg attgatctcg gcgatcgtta
    16741 ccgtctgctg gttaactgta tcgacacggt gaaaacaccg cactccctgc cgaaactgcc
    16801 ggtggcgaat gcgctgtgga aagcgcaacc ggatctgcca actgcttccg aagcgtggat
    16861 cctcgctggt ggcgcgcacc ataccgtctt cagccatgcg ctgaacctca acgatatgcg
    16921 tcagttcgcc gagatgcacg acattgaaat cacggtgatt gataacgaca cccgcctgcc
    16981 agcgtttaaa gacgcactgc gctggaacga agtgtattac ggatttcgtc gctaagtagc
    17041 cacatcaggt atgtaacgcc tgatgcgacg ctgacgcgtc ttatctggcc tacacgctgc
    17101 gattttgtag gccggataag caaagcgcat ccggcattca acgcctgatg cgacgctggc
    17161 gcgtcttatc aggcctacgc gctgcgattt tgtagaccgg ataagcgaag cgcatccggc
    17221 acgaaggagt caacatgtta gaagatctca aacgccaggt attagaagcc aacctggcgc
    17281 tgccaaaaca caacctggtc acgctcacat ggggcaacgt cagcgccgtt gatcgcgagc
    17341 gcggcgtctt tgtgatcaaa ccttccggcg tcgattacag cgtcatgacc gctgacgata
    17401 tggtcgtggt tagcatcgaa accggtgaag tggttgaagg tacgaaaaag ccctcctccg
    17461 acacgccaac tcaccggctg ctctatcagg cattcccctc cattggcggc attgtgcata
    17521 cgcactcgcg ccacgccacc atctgggcgc agtcgggtca gtcgattcca gcaaccggca
    17581 ccacccacgc cgactatttc tacggcacca ttccctgcac ccgcaaaatg accgacgcag
    17641 aaatcaacgg cgaatatgag tgggaaaccg gtaacgtcat cgtagaaacc tttgaaaaac
    17701 agggtatcga tgcagcgcaa atgcccggcg ttctggtcca ttcccacggc ccgtttgcat
    17761 ggggcaaaaa tgccgaagat gcggtgcata acgccatcgt gctggaagag gtcgcttata
    17821 tggagatatt ctgccgtcag ttagcgccgc agttaccgga tatgcagcaa acgctgctgg
    17881 ataaacacta tctgcgtaag catggcgcga aggcatatta cgggcagtaa tgactgtata
    17941 aaaccacagc caatcaaacg aaaccaggct atactcaagc ctggtttttt gatggatttt
    18001 cagcgtggcg caggcaggtt ttatcttaac ccgacactgg cgggacaccc cgcaagggac
    18061 agaagtctcc ttctggctgg cgacggacaa cgggccgttg caggttacgc ttgcaccgca
    18121 agagtccgtg gcgtttattc ccgccgatca ggttccccgc gctcagcata ttttgcaggg
    18181 tgaacaaggc tttcgcctga caccgctggc gttaaaggat tttcaccgcc agccggtgta
    18241 tggcctttac tgtcgcgccc atcgccaatt gatgaattac gaaaagcgcc tgcgtgaagg
    18301 tggcgttacc gtctacgagg ccgatgtgcg cccgccagaa cgctatctga tggagcggtt
    18361 tatcacctca ccggtgtggg tcgagggtga tatgcacaat ggcactatcg ttaatgcccg
    18421 tctgaaaccg catcccgact atcgtccgcc gctcaagtgg gtttctatag atattgaaac
    18481 cacccgccac ggtgagctgt actgcatcgg cctggaaggc tgcgggcagc gcatcgttta
    18541 tatgctgggg tcggagaatg gcgacgcctc ctcgcttgat ttcgaactgg aatacgtcgc
    18601 cagccgcccg cagttgctgg aaaaactcaa cgcctggttt gccaactacg atcctgatgt
    18661 gatcatcggt tggaacgtgg tgcagttcga tctgcgaatg ctgcaaaaac atgccgagcg
    18721 ttaccgtctt ccgctgcgtc ttgggcgcga taatagcgag ctggagtggc gcgagcacgg
    18781 ctttaaaaac ggcgtctttt ttgcccaggc taaaggtcgg ctaattatcg acggtatcga
    18841 ggcgctgaaa tccgcgttct ggaatttctc ttcattctcg ctggaaactg tcgctcagga
    18901 gctattaggc gaaggaaaat ctatcgataa cccgtgggat cgaatggacg aaattgaccg
    18961 ccgtttcgcc gaagataaac ctgcgctggc aacttataac ctgaaagatt gcgagctggt
    19021 gacgcagatc ttccacaaaa ctgaaatcat gccattttta ctcgaacggg caacggtgaa
    19081 cggcctgccg gtggaccgac acggcggttc ggtggcggca tttggtcatc tctattttcc
    19141 gcgaatgcat cgcgctggtt atgtcgcgcc taatctcggc gaagtgccgc cgcacgccag
    19201 ccctggcggc tacgtgatgg attcacggcc agggctttat gattcagtgc tggtgctgga
    19261 ctataaaagc ctgtacccgt cgatcatccg cacctttctg attgatcccg tcgggctggt
    19321 ggaaggcatg gcgcagcctg atccagagca cagtaccgaa ggttttctcg atgcctggtt
    19381 ctcgcgagaa aaacattgcc tgccggagat tgtgactaac atctggcacg ggcgcgatga
    19441 agccaaacgc cagggtaaca aaccgctgtc gcaggcgctg aaaatcatca tgaatgcctt
    19501 ttatggcgtg ctcggcacca ccgcctgccg cttcttcgat ccgcggctgg catcgtcgat
    19561 caccatgcgt ggtcatcaga tcatgcggca aaccaaagcg ttgattgaag cacagggcta
    19621 cgacgttatc tacggcgata ccgactcaac gtttgtctgg ctgaaaggcg cacattcgga
    19681 agaagaagcg gcgaaaatcg gtcgtgcact ggtgcagcac gttaacgcct ggtgggcgga
    19741 aacgctgcaa aaacaacggc tgaccagcgc attagaactg gagtatgaaa cccatttctg
    19801 ccgttttctg atgccaacca ttcgcggagc cgataccggc agtaaaaagc gctacgccgg
    19861 gctgattcag gagggcgata aacagcggat ggtgtttaaa gggctggaaa ccgtgcgcac
    19921 cgactggacg ccgctggccc agcagtttca gcaggagcta tacctgcgca tcttccgcaa
    19981 cgagccatat caggaatatg tacgcgaaac catcgacaaa ctgatggcgg gtgaactgga
    20041 tgcgcgactg gtttaccgta aacgccttcg ccgtccgctg agcgagtatc agcgtaatgt
    20101 gccgcctcat gtacgcgccg ctcgccttgc cgatgaagaa aaccaaaagc gtggtcgccc
    20161 cttgcaatat cagaatcgcg gcaccattaa gtacgtatgg accaccaacg gcccggagcc
    20221 gctggactac caacgttcac cactggatta cgaacactat ctgacccgcc agctacaacc
    20281 cgtggcggag ggaatactcc cttttattga ggataatttt gctacactta tgaccgggca
    20341 acttgggcta ttttgagcaa aaaaagagtt cgccagatac cattttgatg cgtgacgaat
    20401 gctttgccat ccagtaccat agcgcccttt ccattcctgg acctgaataa caccactacc
    20461 tcataagcac ggtagcgggt ggttattgcc tgcaattaaa gatatagagc cgaacacata
    20521 tgccttttac acttggtcaa cgctggatca gcgatacaga aagcgaattg ggacttggaa
    20581 ccgttgtcgc ggtggatgcg cgaactgtca ctttactttt cccatctact ggtgaaaacc
    20641 gtctgtacgc acgcagtgat tcccccgtga cccgcgtgat gttcaaccct ggtgatacca
    20701 ttaccagcca tgacggctgg cagatgcaag tcgaagaagt aaaagaagaa aatggcttgc
    20761 tgacctatat cggtactcgc ctggatactg aagagtccgg cgtagccctg cgtgaagttt
    20821 tccttgatag caaactggtg ttcagcaaac cgcaggaccg cctgtttgcc gggcagattg
    20881 accgtatgga ccgctttgcg ctgcgttatc gcgcgcgtaa gtattccagc gaacagttcc
    20941 gtatgccgta cagcggcctg cgcggtcagc gtaccagcct gatcccgcac cagctcaaca
    21001 tcgctcatga tgttggccgc cgccacgcgc cgcgcgtcct gctggctgac gaagtgggtt
    21061 tagggaaaac cattgaagcc gggatgatcc tgcatcagca actgctctct ggcgctgctg
    21121 aacgtgtgct gattatcgtc ccggaaacct tacagcatca gtggctggta gaaatgctgc
    21181 gccgtttcaa cctgcgcttt gctctgtttg atgatgagcg ttatgccgaa gctcagcacg
    21241 atgcttacaa cccgttcgac accgaacagc tggtgatttg ctcgctggat tttgcccgtc
    21301 gtagcaaaca gcgcctggaa catctctgtg aagccgaatg ggacctgctg gtggtcgatg
    21361 aagcgcatca cctggtgtgg agcgaagatg cgccaagccg tgaatatcag gccattgaac
    21421 aactggcaga gcacatgccg ggcgttctgc tgctgaccgc gaccccggaa cagctgggga
    21481 tggaaagcca cttcgcccgt ctgcgtctgc tggacccgaa ccgtttccac gattttgcgc
    21541 agttcgttga agagcagaaa aattatcgtc cggttgcgga cgccgttgcc atgctgctgg
    21601 caggtaacaa actgagcaat gacgaactga acatgctcgg cgagatgatc ggcgagcagg
    21661 atatcgagcc gctgttgcag gcagcaaaca gcgacagcga agatgcccag agcgcccgtc
    21721 aggagctggt ttcgatgctg atggatcgcc acggcaccag ccgcgtgctg ttccgtaaca
    21781 cgcgtaacgg tgtgaaagga ttcccgaaac gcgagctgca caccattaag ctgccgctac
    21841 cgacgcagta tcagacggct attaaagtct ccggcattat gggcgcacgt aaaagtgcgg
    21901 aagatcgtgc tcgcgatatg ctctacccgg agcgtattta tcaggaattt gaaggtgata
    21961 acgccacctg gtggaacttc gatccgcgcg ttgagtggct gatgggctac ctgaccagcc
    22021 atcgctctca gaaagtgctg gtgatctgcg ccaaagctgc cactgcgctg caactggagc
    22081 aggtactgcg cgaacgtgaa ggtattcgcg ctgcggtgtt ccacgaaggt atgtcgatta
    22141 tcgaacgtga ccgcgctgcc gcctggtttg ccgaagaaga caccggcgca caggtactgc
    22201 tgtgctcaga aatcggttct gaaggacgta acttccagtt cgccagccac atggtgatgt
    22261 ttgacctgcc attcaacccg gatctactgg agcagcgtat tggtcgtctg gatcgtatcg
    22321 gccaggcgca cgatattcag atccatgtgc cttatctgga gaaaaccgct cagtcggtgc
    22381 tggtgcgctg gtatcacgaa ggtctggatg catttgagca cacctgcccg accggacgca
    22441 ctatttacga tagcgtatac aacgatctga ttaactatct ggcttcaccg gatcaaaccg
    22501 aaggctttga cgatctgatc aaaaactgcc gcgagcaaca tgaagcgctg aaagcacagc
    22561 tggaacaggg tcgtgaccgc ctgctggaaa tccactccaa cggtggcgaa aaagcccagg
    22621 cactggcaga aagcattgaa gagcaggatg acgataccaa cctgatcgcc ttcgccatga
    22681 acctgctcga tattatcggt atcaatcagg acgatcgcgg cgacaacatg atcgtgctga
    22741 cgccgtccga tcatatgctg gtgccggact tccctggcct gtcggaagat ggcatcacca
    22801 tcacctttga tcgtgaagtg gcgctggcgc gtgaagatgc acagtttatt acctgggagc
    22861 atccgctgat ccgcaacggt ctggatctga tcctttctgg cgataccggt agcagcacga
    22921 tttcactgtt aaaaaacaaa gcgttgccgg taggtacgct gttggtggaa ctgatttatg
    22981 tggttgaagc ccaggctccg aagcagttgc agctcaaccg cttcctgcca ccgacgccgg
    23041 tacgtatgct gctggataaa aacggcaaca acctggcggc gcaggtagag tttgaaacct
    23101 ttaaccgcca gcttaacgcg gttaaccgtc acaccggcag caaactggtt aacgccgtgc
    23161 agcaggatgt tcacgctatc cttcaactgg gtgaagcgca gatcgagaaa tctgcccgtg
    23221 cattgattga tgcagcgcgt aacgaagccg acgaaaaact gtctgccgag ctgtctcgtc
    23281 tggaagctct gcgtgcagtg aacccgaaca ttcgtgacga cgaactgacc gccattgaga
    23341 gcaaccgtca gcaggtaatg gaaagcctgg atcaggcagg ttggcgtctg gatgccctgc
    23401 gtttgatcgt tgtaacgcat cagtaacgga gccgaaaatg gggatggaaa actacaatcc
    23461 accgcaggaa ccctggttgg ttatcctgta tcaggatgac cacattatgg tggttaacaa
    23521 gccgagcggt ttgttgtctg tgccggggcg tctggaagag cacaaagaca gcgtgatgac
    23581 gcgcattcag cgtgattatc cgcaggcaga atcggtgcat cgtctggata tggctaccag
    23641 cggcgtgatt gtagtggcgc tgaccaaagc cgcggagcgg gagttaaaac gccagttccg
    23701 cgagcgcgag ccgaaaaagc agtatgtggc ccgcgtctgg gggcatccat cccctgcgga
    23761 aggtctggtg gatctgccgc tgatttgcga ctggccaaac cgcccgaaac agaaagtctg
    23821 ttacgaaacg ggtaaacctg cgcagacgga atatgaagtg gtggagtatg cggcggataa
    23881 cacggcaaga gtggtgttaa aaccgattac tgggcgttcg catcaattgc gtgtgcatat
    23941 gctggcgctg gggcatccga ttctcggcga tcgtttttat gcatcaccag aagcgagagc
    24001 gatggcaccg cgtttgttgc tacatgcaga gatgttgacg attacccatc cggcgtatgg
    24061 caatagtatg acgtttaaag cgccagcgga tttttaatag tcacgctggc gcagttcggt
    24121 aggcggataa ggcgttcacg ccgaatccgg caattgtgcg ccgattgcct gatgcgacgc
    24181 gggccgcgtc ttatcaggcc tacagtttaa aacttattta aaccctttct gctgctttat
    24241 cagctcatat gcctgctgaa tttcctgcgc tttctgcttc gccatctcca tcatctcagg
    24301 cggcaaacct ttcgccacca gcttatcggg atggtgttca ctcatcagct tacggtaggc
    24361 tcgtttaatg gtggtcgcat catccgtcgg cttcacaccc agcacattac aggcatcttc
    24421 cagcgtcggg ccacgctgcg cttgctgcca gttaccaccg ccagattgct gctgataacc
    24481 gccgccaaac tgtgcaccgc cctgcatcat gcgcaaaaac tggtcaaact gggcgcggga
    24541 gatccctaat tcttctgcaa tgacatacag caccgcccgt tcattcgggt gcagtgaacc
    24601 atcagcaaac gctgcctgaa tctggatctc cagaaacata cgaattaagt caaaacgacc
    24661 aaagcagaca ctgcgaaact ggcgcatctt ttcgcgcagc gggtaattat ctgattttcc
    24721 cacccggaac gcgttttgcg ccgcagtacg ggaagcgcca tgaaggttca ttcggtccat
    24781 cagctggctg gcgatatgaa tatcagcctc cgtgacgcga cctttggatt tggttaaatg
    24841 ccccatcact tcaaaagtgg tggcaaaaaa cagcgcctga cgctcacgct ggttggcgaa
    24901 ccacgccatt ttacggctac gggctttatc aaacatatgg ccaattaaca ggcctaacac
    24961 tacgccccaa aagccgccgc ccatcagtaa ggccacggcc acgccaatga tttttcccca
    25021 atactgcata tattccccaa atcgacacac ggatatcagg gctatctccc acaatataaa
    25081 ggtgctttta ccgttttccg gctgcagtca attgtgggac atcgcctata atttgcatta
    25141 tcatacctgt cattcaatgc cgtgcctaac accactgacg ctattcggac aggattaaca
    25201 ctagcgtaga gatgacgagt acgttagtct ctgaccgttt gtcacgcgca acgttaccga
    25261 tgatggaaca ataaaatcaa cgtatgaaaa aacgtatccc cactctcctg gccaccatga
    25321 ttgccaccgc cctttatagt caacagggac tggcagccga cctcgcctca cagtgcatgt
    25381 tgggcgtgcc aagctatgac cgtcctctgg tacagggcga taccaatgac ttacccgtga
    25441 ctatcaatgc tgaccacgcg aaaggggact acccggatga cgccgtgttt actggcagcg
    25501 tggatatcat gcagggtaac agccgtctgc aggccgacga agtgcagctc catcaaaaag
    25561 aggcaccagg acaaccggag ccggtacgaa ccgttgatgc gctcggtaat gtccattacg
    25621 acgataacca ggtgatcctc aaagggccga aaggctgggc gaatctgaac accaaagata
    25681 ccaacgtctg ggaaggtgat taccagatgg tgggtcgcca gggccgcggt aaagcggacc
    25741 tgatgaaaca acgtggtgaa aaccgctata ccattctgga taacggtagc tttacctcct
    25801 gtctgccggg ttctgacacc tggagcgtgg taggtagcga aattattcat gaccgcgaag
    25861 aacaagttgc ggagatctgg aacgcccgct ttaaggtggg tccagtaccg atcttttata
    25921 gcccctattt gcagttgccg gtgggtgaca aacgtcgctc tggtttcttg atcccgaacg
    25981 ccaagtacac caccaccaac tactttgagt tctacctgcc atattactgg aacatcgcgc
    26041 caaatatgga tgccaccatc acgccgcatt atatgcatcg tcgtggcaac atcatgtggg
    26101 agaacgaatt ccgctacctc tcccaggcgg gcgctggctt gatggaactg gactatctgc
    26161 cttcagataa agtctatgaa gatgaacacc cgaacgatga cagttcacgt cgttggttgt
    26221 tctactggca acactccggg gtcatggatc aagtgtggcg tttcaacgta gactacacca
    26281 aggtcagcga tcctagctac ttcaatgatt tcgataacaa gtacggttcc agtactgacg
    26341 gctacgcaac gcaaaaattc agcgttggct atgcggtgca aaacttcaat gccaccgttt
    26401 caaccaagca gttccaggtc tttagcgagc agaacaccag tagctactcg gcagagccgc
    26461 agttagacgt taactactac cagaatgatg ttggtccgtt tgatacgcgt atttacggcc
    26521 aggcagtgca ctttgttaac accagagacg acatgcctga agcaacccgt gttcacctgg
    26581 aaccgaccat caatttgccg ctctctaata actggggcag catcaatacc gaagcgaagt
    26641 tgctggcaac ccattatcag caaaccaatc ttgactggta taactccaga aacacgacca
    26701 agctggacga atccgttaac cgcgttatgc cgcaattcaa agttgacggc aaaatggtct
    26761 ttgaacgcga tatggaaatg ctggctccgg gttataccca aacgctggaa ccgcgcgcgc
    26821 agtatttgta cgtgccgtat cgcgatcaga gcgacatcta taactacgac tcgtctctac
    26881 tgcaatctga ctactctggc ctgttccggg accggactta cggcggtctt gaccgtattg
    26941 cctccgctaa ccaggtgacg accggtgtca catctcgcat atatgatgat gctgccgttg
    27001 aacattttaa tatttccgtt ggtcaaatct actatttcac ggagtctcgc actggcgatg
    27061 acaacataac atgggagaat gacgacaaaa cgggctcact ggtgtgggca ggcgatactt
    27121 actggcgtat ctccgagcgt tggggattgc gtggcgggat tcagtacgat acacgtctgg
    27181 ataacgtagc gaccagtaac tccagcattg aataccgtcg ggatgaagac cgtctggtac
    27241 agctgaatta ccgttacgcc agcccggaat atattcaggc tacgctgcct aagtactatt
    27301 ccactgctga gcaatataag aatggtattt cgcaggtagg tgctgtcgcc agctggccaa
    27361 ttgccgatcg ttggtccatt gttggggcct actactacga caccaatgcg aacaagcaag
    27421 ccgactctat gttaggtgtg caatacagct cctgctgcta tgcaattcgc gtcggttacg
    27481 agcggaagct gaacggttgg gataacgata aacaacatgc ggtatatgac aacgcaatcg
    27541 gctttaacat cgaacttcgc ggcctgagct ccaactacgg tctgggtacg caagagatgc
    27601 tgcgttcgaa cattctgccg tatcaaaaca ctttgtgatt tgttgattta ccacgtaatc
    27661 cgcagtgcgg ttaattgaaa tggaaaaagt atgaagaact ggaaaacgct gcttctcggt
    27721 atcgccatga tcgcgaatac cagtttcgct gccccccagg tagtcgataa agtcgcagcc
    27781 gtcgtcaata acggcgtcgt gctggaaagc gacgttgatg gattaatgca gtcggtaaaa
    27841 ctgaacgctg cgcaggcaag gcagcagctt cctgatgacg cgacgctgcg ccaccaaatc
    27901 atggaacgtt tgatcatgga tcaaatcatc ctgcagatgg ggcagaaaat gggagtgaaa
    27961 atctccgatg agcagctgga tcaggcgatt gctaacatcg cgaaacagaa caacatgacg
    28021 ctggatcaga tgcgcagccg tctggcttac gatggtctga actacaacac ctatcgtaac
    28081 cagatccgca aagagatgat tatctctgaa gtgcgtaaca acgaggtgcg tcgtcgcatc
    28141 accatcctgc cgcaggaagt cgaatccctg gcgcagcagg tgggtaacca aaacgacgcc
    28201 agcactgagc tgaacctgag ccacatcctg atcccgctgc cggaaaaccc gacttctgat
    28261 caggtgaacg aagcggaaag ccaggcgcgc gccattgtcg atcaggcgcg taacggcgct
    28321 gatttcggta agctggcgat tgctcattct gccgaccagc aggcgctgaa cggcggccag
    28381 atgggctggg gccgtattca ggagttgccg ggtatcttcg cccaggcatt aagcaccgcg
    28441 aagaaaggcg acattgttgg cccgattcgt tccggcgttg gcttccatat tctgaaagtt
    28501 aacgacctgc gcggcgaaag caaaaatatc tcggtgaccg aagttcatgc tcgccatatt
    28561 ctgctgaaac cgtcgccgat catgactgac gaacaggccc gtgtgaaact ggaacagatt
    28621 gctgccgata tcaagagtgg taaaacgact tttgctgccg cagcgaaaga gttctctcag
    28681 gatccaggct ctgctaacca gggcggtgat ctcggctggg ctacaccaga tattttcgat
    28741 ccggccttcc gtgacgccct gacccgcctg aacaaaggtc aaatgagtgc accggttcac
    28801 tcttcattcg gctggcattt aatcgaactg ctggataccc gtaatgtcga taaaaccgac
    28861 gctgcgcaga aggatcgtgc ataccgcatg ctgatgaacc gtaagttctc ggaagaagca
    28921 gcaagctgga tgcaggaaca acgcgccagc gcctacgtta aaatcctgag caactaatgg
    28981 ttaaaaccca acgtgttgtg atcactcccg gcgagcccgc cgggattggc ccggacttag
    29041 ttgtccagct tgcacagcgt gagtggccgg tcgaactggt tgtttgtgcc gatgccactc
    29101 tccttaccga ccgggcagcg atgctcggtt tgccgctcac cctccgccct tattccccca
    29161 actcccctgc acaaccgcaa actgcgggca cattaacgct acttcctgtc gcgctacgcg
    29221 aatctgtcac tgcggggcag ttagcgattg aaaatgggca ttacgtggtg gagacgctgg
    29281 cgcgagcgtg tgatggctgt ctgaacggtg aatttgctgc gctgatcaca ggccccgtgc
    29341 ataaaggcgt cattaacgac gcaggtattc cgtttactgg tcataccgag tttttcgaag
    29401 agcgctcgca ggcgaaaaaa gtggtgatga tgctggcgac cgaagaactt cgcgtggcgc
    29461 tggcaacgac gcatttaccg ctgcgcgata tcgcagatgc tatcacccct gcacttttgc
    29521 acgaagtgat tgctattttg catcacgatt tgcgtacaaa atttggtatt gccgaaccgc
    29581 gcattctggt ctgcgggctg aatccgcacg cgggcgaagg cggtcatatg ggtacggaag
    29641 agatagacac cattattccg gtgctcgacg agctgcgggc gcaggggatg atactcaacg
    29701 ggccgctgcc tgccgatacc ctgtttcagc cgaaatatct cgataacgcc gacgccgtgc
    29761 tggcgatgta ccacgatcag ggtcttcccg tgctaaaata ccagggcttc gggcgcggtg
    29821 tgaacattac gctgggcctg ccctttattc gcacatcagt ggaccacggc accgcgcttg
    29881 aactggcggg acgtggcgaa gccgatgtcg gcagttttat tacggcgctt aatctcgcca
    29941 tcaaaatgat tgttaacacc caatgaataa tcgagtccac cagggccact tagcccgtaa
    30001 acgcttcggg caaaactttc tcaacgatca gttcgtgatc gacagcattg tttctgccat
    30061 taacccgcaa aagggccagg caatggtcga aatcggcccc ggtctggcgg cattgaccga
    30121 gccggtcggc gaacgtctgg accagctgac ggtcatcgaa cttgaccgcg atctggcggc
    30181 acgtctgcaa acgcatccat tcttaggccc gaaactgacg atttatcagc aggatgcgat
    30241 gacctttaac tttggcgaac tggccgagaa aatgggtcag ccgctgcgtg ttttcggcaa
    30301 cctgccttat aacatctcca cgccgttgat gttccatctg tttagctata ctgatgccat
    30361 tgccgacatg cactttatgt tgcaaaaaga ggtggtgaat cgtctggttg caggaccgaa
    30421 cagcaaagcg tatggtcgat taagcgtcat ggcgcaatac tattgcaacg tgatcccggt
    30481 actggaagtt ccgccgtcag cctttacacc accacccaaa gtggattccg ccgtcgtgcg
    30541 cctggttcct catgcaacga tgcctcaccc ggttaaagat gttcgtgtgt tgagccgcat
    30601 caccaccgaa gcctttaacc agcgtcgtaa aaccattcgt aacagcctcg gcaacctgtt
    30661 tagcgtcgag gtgttaacgg gaatggggat cgacccggcg atgcgagcgg aaaatatctc
    30721 tgtcgcgcaa tattgccaga tggcgaacta tctggcggag aacgcgcctt tgcaggagag
    30781 ttaacgatga tcaattcgcc ccgagtgtgt attcaggttc aaagcgtcta cattgaggca
    30841 caatcttcac ctgataatga acgttacgtt tttgcttata ccgtaaccat acgcaatctg
    30901 gggcgagcgc cagtgcagtt gttggggcgt tactggctga tcaccaatgg caatggccgt
    30961 gaaaccgaag tccagggcga aggagtggtt ggcgtccagc cacttatcgc gcctggcgaa
    31021 gagtaccagt acaccagcgg tgcaatcatt gaaaccccgc tgggcaccat gcagggtcac
    31081 tacgaaatga tcgatgaaaa tggcgtccct ttcagcatcg acattcccgt attccgactc
    31141 gccgttccca cactcattca ttaaaagaat atggcgacat accttattgg cgacgttcat
    31201 ggttgttacg atgaactgat cgcattgctg cataaagtag aatttacccc tgggaaagat
    31261 accctctggc tgacgggcga tctggtcgcg cgcggcccgg gttcgctgga tgttctgcgc
    31321 tatgtgaaat ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga tctgcatctg
    31381 ctggcggtat ttgccgggat cagccgcaat aaaccgaaag atcgcctgac accgctgctg
    31441 gaagcgccgg atgccgacga gctgcttaac tggctgcgtc gccagccttt gctgcaaatc
    31501 gacgaagaga aaaagttggt gatggcccac gcagggatca cgccgcagtg ggatctacag
    31561 accgccaaag agtgcgcgcg cgatgtagaa gcggtgctat cgagtgactc ctatcccttc
    31621 tttctcgacg ctatgtacgg cgatatgcca aataactggt caccggaatt gcgggggctg
    31681 ggaagactgc gttttatcac caacgccttt acccgtatgc gtttttgctt cccgaacggt
    31741 caactggata tgtacagcaa agaatcgccg gaagaggccc ctgccccact gaaaccgtgg
    31801 tttgcgattc ctggccctgt cgctgaagaa tacagcatcg cctttggtca ctgggcatcg
    31861 ctggagggca aaggtacgcc ggaaggtata tacgcgctgg ataccggctg ctgctggggt
    31921 ggtacattaa cctgcctgcg ctgggaagat aaacagtatt ttgtccagcc gtcgaaccgg
    31981 cataaggatt tgggcgaagc ggcggcgtct taaacacagc ctgatatagg aaggccggat
    32041 aagacgcgac cggcgtcgca tccggcacta gccgtaaatt ctatacaaaa ttaccgccgc
    32101 tccagaatct caaagcaata gctgtgagag ttctgcgcat cagcatcgtg gaattcgctg
    32161 aataccgatt cccagtcatc cggctcgtaa tccgggaaat gggtgtcgcc ttccacttct
    32221 gcgtcgatat gcgtcagata cagtttctgc gcttttggca ggaactgctc ataaacgcga
    32281 ccgccgccaa tcaccatgat ttctggtacg tcaccacacg ccgcgatggc ttcatccacc
    32341 gacttcaccc acgttacgcg atcgtccgta cccggttgac tgctgaggat aatatttttg
    32401 cgtcctggca acggacgacc gattgattcc caggtatggc ggcccataat cacgggttta
    32461 tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca ggttccacgg catggcgttt
    32521 tccatgccga taacgcgatc taccgctaac gccgcaatca gactgatcat tgagatttcc
    32581 cgataaaaaa aattgtcgcc actatacgta aagcgtaaac cgtcgtcgac tggtgcgagg
    32641 atgatgttga ggaaaatttt atattctgct ggcgagtcca cgctctctcc ctggactcgc
    32701 cgcattacaa tgaaacaaaa acaaacagtt agctgtaaag tgtgatttat gtcactcttt
    32761 attaggatga gggtttcgtt tccggttcat ccgccatgtt gccggtatgt ttaccttctt
    32821 cggttccctg ccagccatgt cgttgaatta atgacagatg ttcgcggtcc tcggtaatga
    32881 tctcacttaa catcgcgctg gtgcgtttat agaccgccgc gcgggctttg gtgtcgttct
    32941 caaccattgc catctcttcc accatctgaa tattaaagcg gcggaacaca tcggcacgtt
    33001 ctcgcgcttc atacggcccc agacctaaac tttccagtgc cagacgcccg gttttcagcg
    33061 caccttcgaa ggtttcacgc tccggctttt caacgcctgc ctgacgcaaa cgaatgtagt
    33121 ggtcgacatc gcgggcgcgg gcaataatct gcaaatgcgg gaagtgttct ttcaccatct
    33181 ctgtcagttg caggttggtt tgcggatcgt cgatggcgtt aatcagcact tccgctttcg
    33241 ccgctcccgc agattccagt aaatccatcc gcgtggcatc gccataaaac actttcatac
    33301 caaatttacg caaggtttcg atatggtccg gatcgtgatc gagcaccacc attttcaccc
    33361 cgctggagag cagtaaacgt ccggtgatct gcccaaaacg accgaatccg gcaatgatta
    33421 cccgcggctg ttcttcgtcg atctcatcgg cttcacgcgc ctcttcagta gaagattgct
    33481 caaggcgatt gaggatcacc agcagaatcg gcgttgccgc catcgacagc gccaccgcca
    33541 gggtcagcga tttcgcccac tccggctcca gcacattcgc catctgcgcc gcgccaaata
    33601 ccacaaaggc aaactcactg ccctgcccta acaacaccgc aaaccaacga cgctgtttat
    33661 ttggcacttg caacggtcgg gcaatcagcc acagcatggc gattttgatg atgaggaaac
    33721 cgagcagcaa aatgacaatg cgcaatgggt tttcaagcag cgtgccaaag tctatcgaca
    33781 tgccaacacc gatgaaaaac agccccaaca gcaaaccttt aaagggttcg atatcgctct
    33841 ccagcgcatg acggtattcc gagcttgcca gcagtacgcc cgccagaaac gcgcccatcg
    33901 ccatcgacaa gccgacctct tccagcagca aaccaaagcc aaacacgagg aataacgcca
    33961 cggcactaaa cacttcccgc aagccagagc gggctacaaa acgcagcgcc ggacgcgtga
    34021 catagcgccc cagcaatacc accagcacca gcgcacccgc cacttttaac gccgagagag
    34081 caaatgcgcc catcgtcgtc gaggcactgc tcgttgccag tagcggaatc atcgccacca
    34141 gcgggatcgc cgcgatatcc tggaacagca gcaccgcaaa ggcactgcga cccatttgcg
    34201 tcaccatcag attgcgttca ttcatcgcct gcatggcaat cgccgtagag gagagcgcca
    34261 gcgtcatgcc gatcaattcc gcgacctgcc agcgcagccc aagtaacatg cagaacagcc
    34321 ccagcaggcc gccgcaaatc accatctgca atgcgccacc gccgaacact gccgcacgca
    34381 gcttccacag cctttgtgga tcgagttcga ggccgataat aaacagcatc agcaccaccc
    34441 caatctcggc aaagtgcaga atagattcgg catcggtcac cagtcgcagc ccccacgggc
    34501 caataatgca gccggcgatc aggtagccaa gtaccgatcc cagaccaaga cgtaccgcaa
    34561 tgggtacaat cagcgctgcc gaaccgagat aaatcagcgc ctgaatcagc gtatggctat
    34621 ccatgatggg cctcctgcca ttccagcaga cgttgcttat agtgacgcgc ctgcccttcg
    34681 agggtttcgt cgtcacaaat aaaggtgcag tgcatggcaa acggtggcag ccagttcagc
    34741 ccgcagtaga ttgccgtcgc ctgtagcggc tgcgacagca catcaaagcc cggatgcgca
    34801 ccaatttcaa aatggctttc cccgccgccg gtcgtcaccg cccacagcaa atgtttgcca
    34861 tgcagcgcct tgccgccgtg accgtaagcc cagccgtgcg acaaaacttt atcgatccaa
    34921 agtttaagaa gcggaggaat gctgtaccac tgcatcggat gctgccagac gatcagatcg
    34981 gcgcgagaca gcgcctcctg ctcggcggca atatcgatat tgaagtcagg atagagttga
    35041 taaagagagc gaatttcgac gccttccagc gtccttgcct gttcaagcat ccgtttattc
    35101 gcatgggaat gatgcggata cggatgcgca taaattataa gaatcatagg tagcccgcgt
    35161 gattacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt atagtcaata
    35221 atattgattc agaaaagaga gaaatctgaa caatttaccc cattctctgc ccgcggatcg
    35281 tcatattgct ggtttgcgcc aatgacatcc ctcgatgtct ccggggcaaa cgctacggaa
    35341 atcaacaagc caaacagcga gatacccgcc cccattagca tcgtgttact gataccgtaa
    35401 ttattgataa agatcggcag tgcccaggtc gaaacaatgg tgccaatacg gcttaaggac
    35461 ataatcacgc ccacggcaga ggcgcggata tctgtcggga agagttcatt aggatagagc
    35521 cactgcaaat tacccggccc gccagagaaa aaggcataca ccgcaaaggc catcactacc
    35581 agccagatcc ccatatccgg gatcagcccc aaaaccgcca gctccagcgt catcatggca
    35641 aagctgccaa tcaacaatgg acgccgtccg gcagtgttta accacagcat cggcggaata
    35701 cagccgagca taaagaacag gctaatcacc acgttcccca gcgccgcgtt tttgccaaca
    35761 cccaatccca acaaaccaac gatttgcggg ccaaaggtgt aaatggcgaa cattgggatc
    35821 acctggcagg tccagatggc ggcaacaaac agaacaaaag gaaaatggcg gcgattaaac
    35881 agatcgcgaa aacgggtttg ctgcggctgc tcttcatcga aagccaccgg ttcgccaaac
    35941 agttttatca tcatctcttc gcactctttt actcgccctt tgcgtaataa ccagcgggga
    36001 gattcaggca gttcgaatcg accaatcaaa atcaacaaac aggggatcgc cgcgctaccc
    36061 agcatccagc gccagccgcc ttccacatca taaagccagt agccgaccag atcggcgcag
    36121 gtcgcgccga cataccacat ggcggcgata aagctgatgg aaaatgcccg ctgacgggta
    36181 ctggagaact cggtgatcat tgaggtggcg atgggataat cggcaccgat gacaatgccg
    36241 ataagtaccc gcatcaccaa cagttcgacg ggggatgaaa caaacatcgt cgccaccgat
    36301 atcacgccga tggcgatgat atcaatgagg aacattttgc gccgtccgac tttatcggaa
    36361 atatagccaa acagcgatgt gccaacgaac agcccggcga gcgttcccgc gcccaacaag
    36421 ccaatccagt cagcgtccag ttttagcgcc ggagtcagtt gctccagcgc cacgccaatc
    36481 attaccagta cataaccatc ca
//
LOCUS       DAJTSL010000022        35594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTSL010000022 DAJTSL010000000
VERSION     DAJTSL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.022"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(291..728)
                     /locus_tag="OO433_000958"
     CDS             complement(291..728)
                     /locus_tag="OO433_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6049523.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKEQRDKATSTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASGM
                     GNGESPRLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1027..1560)
                     /locus_tag="OO433_000959"
     CDS             complement(1027..1560)
                     /locus_tag="OO433_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6049524.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLSEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(1624..1974)
                     /locus_tag="OO433_000960"
     CDS             complement(1624..1974)
                     /locus_tag="OO433_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR6049525.1"
                     /translation="MTQNYKLIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQNVWITYKGKLPG
                     RITGSLKTPPESQP"
     gene            complement(1979..2194)
                     /locus_tag="OO433_000961"
     CDS             complement(1979..2194)
                     /locus_tag="OO433_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6049526.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(2344..4197)
                     /locus_tag="OO433_000962"
     CDS             complement(2344..4197)
                     /locus_tag="OO433_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6049527.1"
                     /translation="MAFKHYDVVRAASPSDLAKQLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWQTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTVGLNALTLTDITKNATYGVEIESLVLEINAPASS"
     gene            complement(5173..5249)
                     /locus_tag="OO433_000963"
     tRNA            complement(5173..5249)
                     /locus_tag="OO433_000963"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5213..5215),aa:Arg,seq:tct)
     gene            complement(5263..5339)
                     /locus_tag="OO433_000964"
     tRNA            complement(5263..5339)
                     /locus_tag="OO433_000964"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5303..5305),aa:Arg,seq:tcg)
     gene            complement(5347..5422)
                     /locus_tag="OO433_000965"
     tRNA            complement(5347..5422)
                     /locus_tag="OO433_000965"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5387..5389),aa:Ile,seq:cat)
     gene            complement(5472..6522)
                     /locus_tag="OO433_000966"
                     /pseudo
     CDS             complement(5472..6522)
                     /locus_tag="OO433_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(6630..6870)
                     /locus_tag="OO433_000967"
                     /pseudo
     CDS             complement(6630..6870)
                     /locus_tag="OO433_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7097..7918)
                     /locus_tag="OO433_000968"
     CDS             complement(7097..7918)
                     /locus_tag="OO433_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6049528.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
     gene            complement(7915..8295)
                     /locus_tag="OO433_000969"
     CDS             complement(7915..8295)
                     /locus_tag="OO433_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6049529.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(8296..9279)
                     /locus_tag="OO433_000970"
     CDS             complement(8296..9279)
                     /locus_tag="OO433_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6049530.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLAEDKSLLPFFS
                     DERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLGQLAHQNLFAWMIDVIRHAMNGSQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQNPPQEKTVVSI
                     AVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGYGLGGMGTKA
                     DDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            complement(9353..9625)
                     /locus_tag="OO433_000971"
     CDS             complement(9353..9625)
                     /locus_tag="OO433_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049531.1"
                     /translation="MAHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(9793..9948)
                     /gene="hokD"
                     /locus_tag="OO433_000972"
     CDS             complement(9793..9948)
                     /gene="hokD"
                     /locus_tag="OO433_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6049532.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(10186..10851)
                     /locus_tag="OO433_000973"
     CDS             complement(10186..10851)
                     /locus_tag="OO433_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6049533.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(11026..11451)
                     /locus_tag="OO433_000974"
     CDS             complement(11026..11451)
                     /locus_tag="OO433_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6049534.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRLSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNVRYFRRY"
     gene            complement(11467..12237)
                     /locus_tag="OO433_000975"
     CDS             complement(11467..12237)
                     /locus_tag="OO433_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCR6049535.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRKGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(12259..13001)
                     /locus_tag="OO433_000976"
                     /pseudo
     CDS             complement(12259..13001)
                     /locus_tag="OO433_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(13008..13970)
                     /locus_tag="OO433_000977"
     CDS             complement(13008..13970)
                     /locus_tag="OO433_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6049536.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASYDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(13993..14418)
                     /locus_tag="OO433_000978"
     CDS             complement(13993..14418)
                     /locus_tag="OO433_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6049537.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDPPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(14402..14725)
                     /locus_tag="OO433_000979"
     CDS             complement(14402..14725)
                     /locus_tag="OO433_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6049538.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            14850..15326
                     /locus_tag="OO433_000980"
     CDS             14850..15326
                     /locus_tag="OO433_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049539.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKKAIVAFILSGNATEPSWVDYDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            15645..15797
                     /locus_tag="OO433_000981"
     CDS             15645..15797
                     /locus_tag="OO433_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6049540.1"
                     /translation="MDTIELGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LTRNTD"
     gene            15809..16129
                     /locus_tag="OO433_000982"
     CDS             15809..16129
                     /locus_tag="OO433_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049541.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKLLSDNQDLHEKYFLDLMDIIDKGFGLLMKKKGIRIEPLENYFATKSINS
                     FDSK"
     gene            complement(16107..16382)
                     /locus_tag="OO433_000983"
     CDS             complement(16107..16382)
                     /locus_tag="OO433_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049542.1"
                     /translation="MSTENDEIINSLIRQINNFDKALQHAAARSDITLLAISFLASVM
                     DKNEVVRQSLVDYIDSLQPGTFNHESFNHEKEHVKSVINSLILNQKN"
     gene            16944..17132
                     /locus_tag="OO433_000984"
     CDS             16944..17132
                     /locus_tag="OO433_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCR6049543.1"
                     /translation="METLLPNINTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            17129..17320
                     /locus_tag="OO433_000985"
     CDS             17129..17320
                     /locus_tag="OO433_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCR6049544.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            17413..19884
                     /locus_tag="OO433_000986"
     CDS             17413..19884
                     /locus_tag="OO433_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR6049545.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RTNQQHSENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPGNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSV
                     NQNQQESEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQSGGGNCPDCG
                     AVMGDATYQETFDEENQVEVQENDPKEMEGAEHPHKENAGSAQDHASDSETGETADPL
                     IAMNGHHVITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDVIKRWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYISVIWQKLIPNQADF"
     gene            19949..20197
                     /locus_tag="OO433_000987"
     CDS             19949..20197
                     /locus_tag="OO433_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR6049546.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            20175..21305
                     /locus_tag="OO433_000988"
     CDS             20175..21305
                     /locus_tag="OO433_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6049547.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRRLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(21351..21989)
                     /gene="ompW"
                     /locus_tag="OO433_000989"
     CDS             complement(21351..21989)
                     /gene="ompW"
                     /locus_tag="OO433_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6049548.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            22229..22318
                     /locus_tag="OO433_000990"
                     /pseudo
     CDS             22229..22318
                     /locus_tag="OO433_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            22346..23089
                     /locus_tag="OO433_000991"
     CDS             22346..23089
                     /locus_tag="OO433_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6049549.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDSVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            23119..23658
                     /locus_tag="OO433_000992"
     CDS             23119..23658
                     /locus_tag="OO433_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6049550.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            23763..24161
                     /gene="yciA"
                     /locus_tag="OO433_000993"
     CDS             23763..24161
                     /gene="yciA"
                     /locus_tag="OO433_000993"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6049551.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(24201..24929)
                     /gene="tonB"
                     /locus_tag="OO433_000994"
     CDS             complement(24201..24929)
                     /gene="tonB"
                     /locus_tag="OO433_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6049552.1"
                     /translation="MTSMTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTALARLTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            25144..25440
                     /locus_tag="OO433_000995"
     CDS             25144..25440
                     /locus_tag="OO433_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6049553.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            25740..26993
                     /gene="kch"
                     /locus_tag="OO433_000996"
     CDS             25740..26993
                     /gene="kch"
                     /locus_tag="OO433_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6049554.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCLAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(27048..27221)
                     /locus_tag="OO433_000997"
     CDS             complement(27048..27221)
                     /locus_tag="OO433_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6049555.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            27364..28824
                     /gene="cls"
                     /locus_tag="OO433_000998"
     CDS             27364..28824
                     /gene="cls"
                     /locus_tag="OO433_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6049556.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEIVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            28859..29188
                     /locus_tag="OO433_000999"
     CDS             28859..29188
                     /locus_tag="OO433_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6049557.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(29241..30245)
                     /gene="oppF"
                     /locus_tag="OO433_001000"
     CDS             complement(29241..30245)
                     /gene="oppF"
                     /locus_tag="OO433_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6049558.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGIKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(30242..31255)
                     /gene="oppD"
                     /locus_tag="OO433_001001"
     CDS             complement(30242..31255)
                     /gene="oppD"
                     /locus_tag="OO433_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6049559.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTTVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLCACFKPVE
                     ELL"
     gene            complement(31267..32175)
                     /gene="oppC"
                     /locus_tag="OO433_001002"
     CDS             complement(31267..32175)
                     /gene="oppC"
                     /locus_tag="OO433_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6049560.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLM
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(32190..33110)
                     /gene="oppB"
                     /locus_tag="OO433_001003"
     CDS             complement(32190..33110)
                     /gene="oppB"
                     /locus_tag="OO433_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6049561.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(33196..34827)
                     /gene="oppA"
                     /locus_tag="OO433_001004"
     CDS             complement(33196..34827)
                     /gene="oppA"
                     /locus_tag="OO433_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6049562.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            35118..35414
                     /locus_tag="OO433_001005"
     CDS             35118..35414
                     /locus_tag="OO433_001005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049563.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSFSIEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(35525..>35594)
                     /locus_tag="OO433_001006"
     CDS             complement(35525..>35594)
                     /locus_tag="OO433_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049564.1"
                     /translation="FSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         8989 a         9107 c         8317 g         9181 t
ORIGIN      
        1 gtgattacct tttgtctgga aaactgacca ttccacgaag atcagatagg tctatagaat
       61 cttggtttgt catagcgttc gccttacttt gagatgaacc tttgctgcat aggagatcag
      121 cccgtcaagg ctcaccagca ctaactgact actcaaaggc tcattccaaa gggtttggtt
      181 cgacgtggtt gagtgcactg cggtgcgcgg tgaaatacct gtacaaaaat gccccgcatc
      241 tgcgaggcat tttcctgaaa gtcacgtatt aaatttcagt gaaattaaaa ttattttaag
      301 cactgcgtcc tgatgtactc ctgcaggtag ttgacctgcg cggttatctt gtcgattcca
      361 cttcggagac ggtaataatt gagttcagca tctgctgtaa gtcttgggct ttctccattg
      421 cccatgccgc tggctccggt cgttgacttt gcacaggtag cggcgacttg caggcgctta
      481 cgcccagcag aaacatcagc acggaggctt tcgatagtcg cgttagcatc agcaagctcc
      541 tttgtatatc tggcatcgag ttctgctacg tcacgttgac gcttctgcat atcagcgatt
      601 gtggatgtgg ctttatcgcg ctgctctttg taggttatgg cgttatcacg gtaatgattc
      661 agccccagac taagcgcacc acaggccacc agcagggcaa tgatgaccac gcacagtacg
      721 cggttcattt caccaccagc gtatctgacc gatgaaataa ccggaggcca taatcacaaa
      781 caccagccag ataagaatga acttccaggt ggataatttt tcagccatca ctcgaatctc
      841 ccgaatcagt ttgctaaaat caaacacact ttctcctttg acttttccgg agtcaggaaa
      901 cacaaaaccc cgcttgcagc caacaaacgg ggtttttact tttattctct tagtttttgt
      961 cagttcgcag gatttcgtgt tatccgtccg tgtgagcaaa ccgcattttt cagcaaaata
     1021 ttctgcttat ctgtcaattc cccagcacgc cagcgcactc tcctggtcgc gacgggatac
     1081 ctgaccgtaa cagttgtttg agcgaatacg gcagtctctg ccaccgtcct taatccacca
     1141 gcgaatcgcc tcacacgctc ccctgcgatc acctgcatta attcgtttat aaaacgtcga
     1201 cggaaaacac ttaccggggc caatgttgta cggacagaat gacgcgatcc ccgctttctg
     1261 gggttcactc aatggcactc tgatgttttt ctccacccat gccagcgcct tatcacgctc
     1321 aatggcgtta acccggtcgc atttttcctt cgacaacttc atgcccggaa cgacaggttt
     1381 gccatccacc aggatggcac cgcggcaaat ggtccagata cccgcgccat cacggtatgc
     1441 cgtggtgtgg ttaccttcct tttcatccag aaactggtcg agaatgtcag gcgcaggcgc
     1501 accagcggca atcagcgcca gaacggcagc cgacaggccg tatctgattt ttgcgttcat
     1561 ggatatttat caggatttat cggtttctgc ccacggacag gtttatctgt tccggtcagt
     1621 gacttaaggt tgtgattccg gaggagtctt cagagaacca gtaattcttc ccggtagctt
     1681 tcctttgtag gttatccata cattctgcgc ctctaaaatt acggggcgct tttccggcga
     1741 ctgctcatcc ccttcacata acccggcagc aacatccagg aagacctgtc tgatgctcct
     1801 tctggctgct gcctcataaa actccagcgc ggcaccttca acacggtcca gcgagatgtc
     1861 caggtcaaaa atttcaccgt caaagcgttt tttgtcccgt aacgctaaag ttaccgtaac
     1921 tttattctca aaattgcgga tccctttcac aatcagttta tagttttgag tcattgaatt
     1981 actctccccg tgcagcctta cgacggtcct ctctgatttt gaaatacagg ttagtcagat
     2041 atgtcagcag cccaaacagc agactcccca gcacgcctat tgccgcccac tgagacgggg
     2101 aaaccctgtc cagcaactgc aggaaccagt agcccgttcc caccgctgac gtggtgtatg
     2161 acacacctgt tgtgattttt tccatctggt acataccccg tctcccgtta tccggaagct
     2221 gacaacaata aaaaagccac cagttaagta ctgatggctc tgataactca tgcaggcgtc
     2281 tcagacgacc cactgacact accggtgagt ttaacgatac cttccatttg actggctcac
     2341 tttttatgat gatgccggtg catttatctc cagcaccaga ctttctatct caacgccata
     2401 cgttgcattt ttggtaatat ccgtcagcgt cagtgcattt agtcccactg tcagactgtc
     2461 ttttatgacc tggaatgccg ggccagccac tccattcagt ttcggagtaa ccgtggcact
     2521 gccggcggtg aacaccagct ccagcgtctg ccagtcgtta ctgtaattcc cgaactcgcc
     2581 caactttgtg tttccggctt tcttgtgatg catcagattc agtttgccgt ctgtggtctg
     2641 ggtgaagaac gacatcagga acgggttacc agtcccggtc atcgccacga cgtcaggtaa
     2701 cgctacatcg gtatacagat aaattcccag accgaactgg ttgttggtca gtgcgcctga
     2761 cagtcgaaac ttacagctca gtctgccacc ccgtgtcagc agggagactg cgtcatccac
     2821 cgggcgcgtc agggaccagg ctttattgct ctgcttggtg atcttaaata caccatctga
     2881 caactgaatt ccgccattct taatgctcca gccctgcgca gcagcatctc cggctgtcgg
     2941 caacagggag attgtgcgta cggatgcatc ttcagacggc cccgatggcg tgtcgccgcc
     3001 gggcgagggt ttgatttccg gtgccttacc actaatgaag gctaaggtgc gaccggctac
     3061 gttcagaata gcagttgcca tacgatcggg aataatgcca cgacgcgccc atgagctgaa
     3121 atgcgtcggg cgatttgatg atacccagtt tttgttcgtt cgggatgccg aaccgtaata
     3181 accagacccg gcaatatcag gatcttctga cgggttgttt gtcggtgtat taactccgct
     3241 accatcggtc ataaagggaa caaaataaat ctgctgggat tctttacctt tatatgcacc
     3301 atataccact tcatattgcg taccgtgttc ttgtttccac gcgtatgtcg tgtcgccaca
     3361 aatccagggg actgatgccg gacttccacc gtgacactgc gctgccagcc cggcaaggtc
     3421 agcacggaac tgctgtacca ttgcaagaaa tgctgctggc tgctgggcgt aactggcatt
     3481 cgtcatatcg aattccccct gcatccagca tatcgccagc aaaacgtttt tcgggttttt
     3541 ctgcaatgct gccttcgtgc ggaaaagcag atcctgatat aacggcttac ccactcccca
     3601 gcgagccgaa tcctgactgg ctcccgtgga ctcgctgaat gtcccctccg tgccctgggt
     3661 gaatgccgaa ccaccacgac agcatggtac cagcaggatc cccgcattat tagggatata
     3721 cggaagcagt tttttggcaa tatgtaagcc ctgtccgaca cagccgtact gccctttgct
     3781 caggtcagcc cggggatggt taatcgtact catatcctga acatcatgca gacaatggtc
     3841 agcaggaatg atgtcgttaa atacgcatac ttcaccaccg ggagtcactg tgttacgacg
     3901 ggccagttgc ttaatgcgcg gatggggcgc atcgtatgaa tccggaagcg gaagcccttc
     3961 accgtaagcc atggcattgg attgcccggc cagtacgatg acgtagtacc actccggctc
     4021 agttgcacca ctgacgacca catcaccttc tgctgcaatc gcctgcatca gggtataagg
     4081 ggttatggcc accggactac caaacggctg ccagccctcc ttcagttttt gtgtcagttg
     4141 tttcgcaagg tctgacggcg atgccgccct gaccacgtca tagtgtttaa atgccatgaa
     4201 tcctcccggg cgggataatg ttgtgagtca gataaggagc aggctgaagt ccggaagtta
     4261 caggacaatg gcagaaggga gactacagcc cgcaattcga aaaagaccgc gcagttgcgc
     4321 agagtgatta ctatggggta ttattcgcca gctgaaatat tacttcacgt tttattgttt
     4381 attccttgcc gcccgcgtct cccagcgcgg gctttttttg tccataagaa agcccctccg
     4441 gagaggggct aaagccgcgt atctgtatca tcatgcacat ggtgccgggt gcctcccggt
     4501 gagttcagcc cggtgccact aaacccgcgt cattctcgtt ttgataatca gagattatac
     4561 cgtcaccagt cgcccctccg ctcaggggga ttcaccatgc gaaatttttt taacaaatgc
     4621 ccagtctgac aggcaactgt caacttactg aattgtgagc aacatagcat ttaacgggga
     4681 acctgttttc tgcagtaaaa aggcccaccg gagcggatgg gcctggaagg atagcggtca
     4741 tgtgatgccg gtttcccggt aactcagcac cggtatctga gtcaacgttt tctctactgg
     4801 gtcatttccg atacgccctg cctgctgaca ggctttcatc acatctgaaa atatagcacc
     4861 ctgactgata ctgtagtacc taaggttcca gaaactgtga tgtatccggc acagaaaagc
     4921 ccctccggag aggggctgga gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc
     4981 cggtgaattc agtaccagca cctgaatccg cgattatccc atatacctac tcgctgattg
     5041 cccctccgca cagggggatt caccatgcca gtttctttta acaaactccc cgcaaaccag
     5101 acaacagtca accgcctgaa ttgtgagaca tttaaaaaaa agcccgcaaa agcgagccag
     5161 ggaaaataag tgtggcgcgt tgtactggat tcgaaccagt gaccgattgc ttagaaggca
     5221 attgctctgt ccggctgagc taacaacgca ggatacagat aatggaccgc cttcggggac
     5281 ccgaactccg cgcaaccagc ttcgaaggct ggcgctcttt cctgatgagc taatggcggt
     5341 atgtgatggt ggcccttgct ggatttgaac cagcgacctg gcgattatga gtcgctcgct
     5401 ctcaccactg agctaaaggg ccgggagcag aataataatg gtgcgtaatt aattctgcaa
     5461 tctcatccgt ttcaaacgat taaatcctga acttccctga ctgtctgctc aaaacgtccg
     5521 gtctccagtt caacgccaat cgcacgacgc ccgagcgcca gtgcagcttt taccgttgaa
     5581 cctgagccca taaaaaaaat ctgcaaccag gtctcccgga cgactgcttg cgctgattat
     5641 ctgctgcagc atttctgccg gtttttcgca cggatgtttc ccgggataga actgcaccgg
     5701 tttatgtgtc cacacatccg tgtacggcac ctgcgccgtc acaccaaaat accgccgcag
     5761 atgcttatat tcactctgca gctccacata ctgccggttc agtgacgtat acgtatccac
     5821 cagctggtgg tggggctttt ccagttcacc gcgctgatgt ttctcttctg ccacccgggc
     5881 aaacagcgac tgtaatttca gataatcgct ttcgttcggt agctgccact gactggcact
     5941 gaaccagtgc gacaccatgt ttttctttcc tgtggcatct gcaatctgtt ttgccgttat
     6001 ccccagggcc gcgcgcgcat cacgaaagta agaaatcagc ggggccatca catgctgttt
     6061 cagtgcactg cccttcgccg catacccggc atctttcgga cgatacggcc cctgataatg
     6121 ttccgcgaac agaatgcgct ctgtggcggg gaaatacgcc cgcaggcttt ccttgttgca
     6181 tccgttccag cgtccggacg gcttcgccca gataatatgg ttcagcacac tgaagcgttc
     6241 acgcatcatg atttcgatat cagatgccag gcgatgacca cagaacaggt aaagacttcc
     6301 ggcaggtttc agcacccgcc agaactgcgc cagacactgg tccagccact tcaggtaatc
     6361 atcgtcgccc ttccactggt tatcccagcc ctcaggcttc actttaaagt acggcgggtc
     6421 cgtgactatc aggtcaacag aattttcggg taacgaccgg ataaattcca ggcagtcggc
     6481 gttgattaac tcacaactgg atatttttac agtattaagc atggatcatt aagcctgtct
     6541 ctgataggct cattctgctt ttgcgcaaag cagtgggcct gaggtttgct tgtgaaccca
     6601 acgcatgagc agatggctgg tgggtgcccc taacacccac cagccgccca tttaccacaa
     6661 ataaaaaagc cttcactgcg gaaggcgtct gtaacaaccg aactgatagt ctgccagacc
     6721 cgccataacc agctgggtca gtattaactg gcagcgttcg cgtgaaaggt aagtattctg
     6781 tgcaatctcc ccgactgtcg ccggttcggt aacgcttaat tcattaaaca ccactctggc
     6841 ggtttctgtc atatcctgct gttttagcat gtctttttcc cttttccggt taacgtgaca
     6901 caccaataac tcttgtcgaa aaagccagca agctgaaaga caggtattca ccgccaccag
     6961 cgcgtttact gtactgacgc gatttcagtc ataaaaaacc cgccaggcgg cggggtgtaa
     7021 aaaatcttct aacgtcaggc ataaaacgcc catcgttaga gcaaatttac cacagattcg
     7081 ggaaaaatca acaacactat cgcgttaccc tctttaactg ccgctccgcc catgcctctt
     7141 caatgtcaaa ccgaaccacc aacgtatcgt aaaagcgttt cactgatttt ttccacgtat
     7201 caagcgtgat agcactcgtc actttgcata tggcattaaa tgcctccgtt gatggtagtc
     7261 tttcacagcc acgaccacca caacgctggc agtctctgat aacaggcata ccacgtttta
     7321 ccgactcttc acgatgaatg gcgacaccac gcccacggca atccttacag gcggtggaaa
     7381 cctcaccctt tccgccacac tccggacagg caacttttac cacctccctg acttttttcc
     7441 attcttccca gtaagacgga tacacacctt tcgtacactt tgcccatacc ggcggcttac
     7501 catccggata ctggaccttg tttgtaaaaa ctacgctttc aataaatttt tccccatagc
     7561 aacaagggca ctgcttttta ctcgctgcgc tgcgggcata atcctcaaaa gcgtacgaag
     7621 ccataatgcg catcactacc ggttttattt ctgccgggag ttttcttaac gccgccacgc
     7681 gatcacaccg actgagtgca tattctgtca gcaattctgt tgcccgctct ctgtcattca
     7741 tactaatgcc cattttccca aggaacgcag aaaaccccat ctcagcccga ttctgtgtca
     7801 tgccctgcgc ggccatcaca tcagtgatac tcagcgcatc tttcgacgtt gaggccgatg
     7861 catcagtcag gccgggggat tttggggagt agtatttcgg taaatcttcc agtttcattt
     7921 tttgacctgc ccttcaagca ttatggggta aatcttcacc cccagacgtc caccagatac
     7981 tggctgagca cgaacgatat tgatttcatc aaactgctca tcgtccatta gcaaccccgc
     8041 atgcgtcagc gcatccagcg gcgctttcag aatattgtcc aggtcacggc ggcgcttatc
     8101 cggtggttct gcaataattt ttattgccaa ccttccggac aggcttaatt tcagccgctg
     8161 ctggcgaaca ataagcgcca ctgcccggcg ataacgctcc ccggcttttg atacaaaata
     8221 tgtgctgcca cgacgacgcc agtaagtgtt caccgttggc gggtaaggca aaacaaattc
     8281 tatgcgttca gtcatttatg ctttccactt cagaacaccc gaatttctcg cgtgcattaa
     8341 aaaacgaatc agcaacaaca gctggctgcc gtgtttttct tcaaaatctt ttaccccggc
     8401 gtgtagttcg ctatggcatt tacggcacag cggaataaca aacaaatcat cagcctttgt
     8461 tcccatccct cccagtccat aaccaatgat gtgatgcgga tcatctgcct gattgccaca
     8521 cgtcatgcat ttctgcgttt ttacccaacg cgtgtataca ggcatctctt cccgttgtga
     8581 tttctggcgc tggagatact gagccggtga ctccggatca acggcaatgc tgaccaccgt
     8641 cttttcctgt ggcgggtttt gctggtgggc gtgaggcagc ggcgcaagat tttttgtgcg
     8701 ctgcttcagt atgctggtgg cggtctgctc tcccggtacg atgtcgcttt cacggtacat
     8761 tgagcggatt ttttccgcac gcaaccccag cgaacgacgt aataccgctt ccggtagcgc
     8821 gtccgccacc tgattgcgga ccgcccacca ggataattca gccagcgata attcccgttc
     8881 ctgcgagcca ttcattgcat ggcgtatgac gtcaatcatc catgcaaaca ggttttggtg
     8941 agcaagttgc ccgagtgatt cggaggtctg gtcgcgcagc tggttgtcgc agtgccagca
     9001 caacaccatt gcgccggtac cataacggtg aatgacggtt tcactgtggt gataatcgcc
     9061 gtgtggccac tggcaggatt taacatggcg cagtaaccag tcagacaatg cgccagcgcc
     9121 accagcagca cgaatcactc gttcgtcgct gaaaaatggc agtaatgatt tatcctccgc
     9181 cagcggctgg cgaacggcag gaacgacccc ggacggcaga ttacgcatgc ttttcggttc
     9241 cggctccacc agtacccggg tattgtggaa taccggcatg gattcacggc ccggcttaac
     9301 gatcaccagc ccgagttccg gtaccagaac aggtcgaagt aatacccgca cgttacctcc
     9361 agatgcgttg ctggaatgtg cgggacggac gcggtgggcg ttcagagtaa ggaagcctga
     9421 cggagattat ccagtgacga taatcgaggc tgagggcttt cttaatctcg tatccgcgtc
     9481 tgcggtagtt atgaattagc cattcggcct gttcttcagt acatggtggg tgttggtacc
     9541 agtcggtttt aaatgcgtgt gaacgccgcc catgccggat ggcaaggtcg gtatcagaat
     9601 tgtgaaattt ggttttgtgc gccatctgtt ttctctgctg gcgcagcagg tgtcaggtgt
     9661 tcaggctgac gtgcgaattg taaaccagaa tgccagaaaa aaacaaaacc cgccgaaccg
     9721 ggttaagtgc gggtgcgttg aggatgcctg acacatcaga ggtggcgagg gatttctccc
     9781 ccgccaggtc tcttactcct caggttcgta agctgtgaag acagcgacct ccgtctggcc
     9841 ggttcggatt cgtacctcgc agaggtcttt cctcgttacc agtgccgtca ctatgacggt
     9901 taaacagatg acgatcaggg cgattaacat cgccttttgc tgcttcatag cctgcttccc
     9961 cttgcctttc ggcacgtaag aggctaacct acatttgtga gacatagatt gggcctcaga
    10021 ttaatgttaa gcgtcttgca ggacgcgaaa tgttaactgg ggcttttctc tatctgcctt
    10081 tcagtgttca tgcctgagac agatagcctc aagcacccgc agccattcta cttaactcac
    10141 gtcacctcgc caatatgaaa tcaatcagaa aggtgatcca taaaatcact ccttctcttc
    10201 ttttccgtag tggagttggc caattttgat aagagggcgt ccctgagatt tgcggtgtag
    10261 attggtatcg cgcagagaat acacacagcc acaatattcc tgctgataga atttttcgcg
    10321 cttgctgatt tcaatcatac gggacgagcc gccctgcttg cgccagttat aatcccagta
    10381 caccataccc ggataatgcg caacagctcg ccgcccacac tcgttaacct gctgcatatt
    10441 tttccagcgt gaaatgccca gtgaactgct gatcacactg aaaccatttt cagcagcgta
    10501 caacgctgtc cgctcaaaac gcatgtcaaa acacatggta caacggatcc ccctctcagg
    10561 ctcccattcc attcctttgg cacgttcaaa ccagttgtcg gtgtcgtaat cagcatcgat
    10621 aaacggcacg ccgtgttgtt cagcaaagcg aatattttca tccttacgaa ttaaatactc
    10681 tttctgagga tgaatgttcg ggttgtagaa aaagatggtg tagtcgattc ccgaggcctg
    10741 aagcgcctcc atcacttcac cggaacatgg agcacagcaa gagtgcagta gtagtttgtt
    10801 tgccccgttt gggagctcca atttaggccg tttgaaatca gcaatagtca taaatatttt
    10861 tattggggtc atgaaaatag cacagagtgt agcatcagag cagggctatc gggaatatat
    10921 gtctaaatct ggtaatatct ggttttgacg caaagcggac aaccacgctg gctctaccct
    10981 gcgccatgaa aatgtcaatt cacatctgaa ctaatgctct ttaatctagt aacgtctaaa
    11041 atacctaaca tttccttgat aaaatgccag tacacgctgc atagcttcgc tcttccggca
    11101 ctcgcgacag attatattca ggcgcctgtc gtagcggcgt atttcgccgt ctggtaacga
    11161 ccagataagg tccggatcaa ccactgcagg tttcttcacc tttgcccttg agagtttttt
    11221 gcgagcattt tgccagtcct tacgcgcctg ttcagacggg aataacccgt aaccagagtt
    11281 gtatacatcg ccactggcaa ccagctctct ggcgagaaca ctcatcagat atcttgtcgc
    11341 acctgtcttg gcttccagtt gccgcaacgt ctcgcgaccg ctcagacgta caagttcaac
    11401 aacctgccct ttaatttttt cccgctcttc ttgtgtaaat acttttgcca taagcgcctc
    11461 cggcaatcac ttttccgata caacacggcg ggaagaatca gtaatctgtc gaacaatatc
    11521 ccggtgcttg ttcagctccc gcagcgcggc gcagactcgc tcccacttct gaacatcact
    11581 tttcgccctg cgcagcgcca ggtttgccct gcgaagggac ggaaaaatca gctcatctgc
    11641 ttgcgtttcg gtaaacgatg gcaacggctg cacaatgtcc gccacagttt ctgttttaat
    11701 ttcttcctgt gttgcggctt cccggactgg taacgcagca cctgctggct gaggaaaggc
    11761 cttaccatca ctttccgtta ccagcgcggc tttcggctct gctggtaaat tatcgcccgg
    11821 catgcagtaa cgaaatttac cgttctgatt aacgcgtgcc agccgccccg ttgcggttac
    11881 caccgccagc gtggaagcaa ccttgcgagt actgacaccg aacttacccg ccagttcctc
    11941 acacgtttta gccccatcct gaccgataaa ctcaatcatc atgtctgcgg taactttttg
    12001 ttcgacctcc ccggtcagca tatcctgtgc ttcagatttt actggccgct cttcggttac
    12061 ccgggattca ccttcgccag ccagaaacca ggtgtgacca gttttatcaa cgacgccttt
    12121 tcttttgagt tcccacagct cgttgacagc ctcttcacga ctgattccaa ggcgagctgc
    12181 caccacatgt gaagaggctt ttttcagtgc tttcagtgcg tcagatacgg tttccattaa
    12241 aatttcctcc ggacaaaatt acttcacaac cctcatattg ctgacatttg gacgccagct
    12301 atcccagtta aacgtcaccc atcgaccacc gttcatggtc atgcggtcca taatcctctc
    12361 accaagaagc gtactcattg cggcatgatt caggtttgtt aacatcccga cactgcacag
    12421 tgatgctgtc cggcgatcaa ttatctggtg caataccacc tgctcgtttt tcgtctcccg
    12481 ctgaacgcct atttcatcca ggaccagcaa atcaaccccg caaagctcct gtatttttcc
    12541 ccggatttgc cgttgtcgta gctgtcatgc aacacgctca tgacgtcaga cacggtgacg
    12601 ataatcacgc tgcgcccctt caccatcagc cggttgccca tcgccgctgc aaggtgattt
    12661 ttcccggtgc cggttttacc gctgaacaca aaattcgtgc acccggtcat cagttcgtca
    12721 gctatggatt tggcctggct cagcgcgtat ttttgcccgt cgttctgcac ctgataattt
    12781 gcaaacgagc atttgctgtg cagaggctgg atgcccgaac gattcaggat tttttccacc
    12841 cgcaactggc gattctggcg gttaatctcc tcgctgcgtt ttcgtccttc agcaagttgc
    12901 cattcccgcc actcctccac cgtccggtac ggtggaaccg acccctgtgg tgcaagtctg
    12961 cgaatacgtt caagaacccc aactgccgca atgtttttca tgacacgtca ccccctgaat
    13021 cccggcggta tttcagtgtc cggttcagaa atgtgattca cacaacgctg cgcaggcgaa
    13081 cgccccaggc ggataaccag ttcatcccat ttttcccgga gttttgccgg actcatgatg
    13141 ttttttaccc agaacgaatc ccgctggaga cgcccaaaca tttcacaaat ttgtctgtga
    13201 gttctgccat ccagcatccg cattgtgcga acgtcattgg cccatgctgt ccagttgggt
    13261 tctttcggtc tagtgatctc gccatcatag ctggccgcct gctcgtaaag actcacgatt
    13321 cgtccccaga tccactgtgc gcacaccaaa tcttcctgac ttccccactg gcgttttttc
    13381 gcactgaaca caaccgcgtc agggtgtcgg gttaaaaaat cctgttcagc cgtctgcggg
    13441 tccggttgcg aagcgtccgg acaagaagat cttttatctg acggatcagg ttttaatact
    13501 gacggatcgg ggtcaatcat cgccccccta atcggcagtt ttttatcaac agttgatcca
    13561 tcaaaatttg acgggtcaac cgttgagggg tcaatatttg acgggtcaac tgttaacggg
    13621 tcattttttg ccgggctaat ttttcttttc ggtttatatg actcacgcgc cgccgccgca
    13681 gctgcttcga gtttttccac attaagccga tagatattgc ttacattacg cccaccgacc
    13741 ttacgctctt ccttcgtcag ccagccctct ttcgccagtt ctgcaatagc cgatttcact
    13801 gtggattcac ttcttgcacc gatctgacgc cggatagttt caatggcagg ccatgacacg
    13861 ccctcgtcat tgctgtagtc tgcaagacgg gccataaccg ccaccctgga taagatcatg
    13921 ccggtgaagg cgcacccttc ccagacaaga ccatgaagct tgctgctcat aaaacccccg
    13981 aacaccgtgc ttttagtgca tcaccacagc attccctgcc gggccgccgc gattcatctg
    14041 gtcatacaaa acaaccgctg acgcaacaaa atcatcgaca tccttcacca gccgatccct
    14101 ccgttcgacg atctcacggt aatattcaga actgtggctg cgcatacggg ccaccagcaa
    14161 aggcggcatc gccttttcga tcgccggtaa cagagcctgc attttttcaa cagcatcagg
    14221 ggggtcttta tccagccaac ggaaaatttt ctgggtatta cgggccaggg cttccggatg
    14281 gctgtcgtcg tacagttcag ggaacgtcat ccccagttcg aaataagtcc gggctatttc
    14341 agctgcagga actttctcac catcagggta tgcccaggca ttcatcgcca tgcggatgtg
    14401 ctcatgtttg attttcatga atcatttgcc tcttgatgct tcgggtatga tcgttttcgt
    14461 catttggttg cttcatcgac atattctgcg aataacatga cgagcgtcgt aagtatgtcc
    14521 aatcaacatc aggacgaagt tcttcacaca ggacaccacc ttttgttgct cgttcaatcg
    14581 caggacatct ctcagcaggt aactgacgta cacctttgat ccattgattt acgcttggag
    14641 gagatacacc taaaagccta gccattgctg attgcccacc gacaacagca caagctcgtt
    14701 tgaatgaata gttatctttt ttcatcgaat gaactccaaa aaacacgcaa caatattagg
    14761 cttagcctaa tgaaattgtc aataggctat gcctaataca tcgagagtag ggattgccta
    14821 acgcgatgcg cataggagac tattaagcaa tgcttagtgg taaagactta ggccgagcga
    14881 tagagcaggc cattaacaaa aaaattgcat caggagccgt caaatcaaag gcggaaatcg
    14941 cacgtcattt caaagtccaa ccaccatcaa tccatgactg gattaagaaa ggttcgataa
    15001 gtaaagacaa acttccagaa ctatggcgtt tcttttctga tgtggttggt ccagagcatt
    15061 gggggcttaa cgaatacccc ataccaaccc catccacttc agatacaaaa agtgaacttt
    15121 tagacataaa cagcctttat caagccgcct ctgatgaaaa aaaagcaatt gtggctttca
    15181 tcttatctgg aaatgctacg gagcctagtt gggttgatta tgacgttcgc gcctacattg
    15241 ccgcaatgga aatgaaggta gctaactatc tgaaaaatca agaatcaaaa cggaaaagcc
    15301 agaacatcac caagacagga acttaaactt atatggtccg acgggaaatt cctagttccc
    15361 gttagttaac tcctactacc tctcccacaa accatcacct attaggttgc gcccaaatta
    15421 ttaggcatag cctattgaca agtaattagg catttcctat agttttccca taccaaccca
    15481 tcccgtccca cacaatacag ggcaatacct cgagttacca ggcagtggtc aggggttaag
    15541 tagccagccc gaggcgtaag aacatgacgg cagggttcaa ctttaataac tatgcagcag
    15601 gtttttgttc cgctaccccg gcgttaaggg gaaatgaggt cagcatggat actatcgagc
    15661 ttggcaacag cgaatctctg gtatgtggcg tgttccccaa ccaggacggt acgttcaccg
    15721 cgatgacgta taccagaagc aaaacgttta aaactgaagc tggcgcgcgt cgctggttaa
    15781 ccagaaacac tgactgatga ggttgacgat ggaatttaaa gatttaccag taccattcca
    15841 ggaaatggca tcgaatgtgg ttcgctctca actggcgact cttgacctga gtaccgtaga
    15901 aaaagaaacc atcgacaata tatccggtaa cgtacgccga gcctttatcg ggctgtacga
    15961 agagaagctg ctctctgata accaggattt acatgaaaaa tacttcctgg atctaatgga
    16021 catcattgat aaggggtttg gcttgttaat gaaaaagaaa gggattcgaa tagaacccct
    16081 tgaaaattac tttgcaacaa aaagcattaa ttcttttgat tcaaaataag agaattaatt
    16141 acagacttaa catgctcttt ctcatgattg aagctctcat gattgaaagt gccgggttga
    16201 agcgagtcga tataatcaac aagactctgt cgtacgactt catttttatc cataacagat
    16261 gcaagaaatg aaattgctaa aagagttata tcactacgcg ccgcagcatg ttgcaatgct
    16321 ttatcaaaat tattaatctg gcgtatcagg gagttaatga tttcatcatt ttcagtcgac
    16381 atttcaccct cctgagggtt ggtgattaag gagttctcca cgggtgaggt ggagtgcgtg
    16441 cgccggacac gggtgagcat ccggcactga cagtttactg aaaggatatt tccctgaaaa
    16501 gtcagaccat aacgcgaaag cgcccggcga ggtagctggt tcatagatag cctgtcgtta
    16561 aattttcgtc gaccgtgcgt ttccggttgt ggcaatccgc gaaatggcgc ggcggtaagt
    16621 atggcggggt tattccttcc ccgttgagga caccgagttg tcaggttgac catacgctta
    16681 agtgacaacc ccgctgcaac gccctctgtt atcaattttc tggtgacgtt tggcggtatc
    16741 agttttactc cgtgactgct ctgccgccct ttttaaagtg aattttgtga tgtggtgaat
    16801 gcggctgagc gcacgcggaa cagttaaaac caaaaacagt gttatgggtg gattctctgt
    16861 atccggcgtt aattgttaac tggttaacgt cacctggagg caccaggcac cgcatcacaa
    16921 aattcattgt tgaggacgcg ataatggaaa cgttattacc aaacattaat acgtctgaag
    16981 gttgttttga aattggtgtc actatcagta accctgtatt tactgaagat gccattaaca
    17041 agagaaaaca cgaacgggag ctattaaata aaatatgcat tctttcaatg ctggcccgtt
    17101 tacgtccgat acaaaaagga tgctggcaat gaatacagca tttgcacttg ttctgacagt
    17161 ttttcttgtt tccggagagc cagttgatat tgcagtcagt gttcacagga caatgcagga
    17221 gtgtgtgact gcagcaaccg aacagaaaat tcccggtaac tgttacccgg tcgataaagt
    17281 tattcaccag gataataacg aaatcccggc aggtctttaa aacagttccg taataaatat
    17341 ccggtttcat tcttatatgc cagcaatggc agggatttgt tcacccttaa atctgtaatg
    17401 aggtaaaaca aaatgagtaa agtctttatt tgcgccgcca ttccggacga acaggcaata
    17461 aaggaagaag gtgccgtcgc tgtagccact gccattgaag ccggtgatga acgtcgcgcc
    17521 cgcgcaaaat ttcactggca attcctggaa cattatccgg ctgctcagga ctgcgcttat
    17581 aaatttcttg tctgcgagga taaacccggt ataccccgcc ctgccctcga ttcctgggat
    17641 gctgaatata tgcaggaaaa ccgctgggat gaggagtctg cttcctttgt cccggttgag
    17701 actgaatcag atccgatgaa cgtcactttt gacaagctgg cccctgaagt acagaacgct
    17761 gtcatggtta agttcgacac atgtgaaaac atcaccgttg atatggttat tagcgcacag
    17821 gaattgttgc aggaagacat ggcaacattc gacggacata tcgttgaagc gttgatgaaa
    17881 atgccagaag ttaacgccat gtatccggag cttaagctgc atgccatcgg gtgggttaag
    17941 cataaatgta agcctggtgc caaatggccc gaaattcagg cagagatgcg catctggaaa
    18001 aaacgtcgcg aaggtgaacg caaggaaacc ggaaaataca cgtctgttgt tgatctcgcc
    18061 cgcgccagaa ccaatcaaca gcacagtgaa aattcaacag gaaaaatcag cccggtcatt
    18121 gctgccattc atcgcgaata caagcagaca tggaaaacac tggatgacga actggcctac
    18181 gctctctggc ctggtgatgt ggatgccgga aacattgacg gcagcatcca tcgctgggca
    18241 aaaaatgaag ttatcgacaa cgaccgcgaa gactggaagc gtatctcggc atcaatgcgc
    18301 aaacagcctg atgcccttcg ctacgaccgc cagactattt ttggccttgt ccgtgaacgt
    18361 ccgatcgaca ttcacaaaga ccctgtggca ctgaacaaat acattactga atacctgact
    18421 acaaagggcg tgtttgaaga tgaaggaaga aatcagagcg caactgatac tctctcgtcg
    18481 ccagtaccag aaactgatgc agtggaaacg gcaattccgg gcaacgaaaa aaccgaatgc
    18541 aaagtggaag tcgaaccatc tgtagagcgt gaggggccgt tctacttcct cttcaccgac
    18601 aaggatggcg aaaaatacgg tcgcgcaaac aaactttctg gtctggaaaa agcactagcc
    18661 ttgggagcta cggaaatcac aaaagaggaa tacttcgcac gtaaaaacgg cacgtactca
    18721 ggttcacaac aaaatactgg tgcatctgac acgaccgcac aaccagagcc ggtaaaagtt
    18781 accgctgacg aagtaaacaa aattatgcag gcagccaata tcagccagcc tgacgccgat
    18841 aagttgcttg ctgcctctcg cggagaattt gttgcaggga ttagcgaccc gaatgatccg
    18901 aaatgggtaa aggggattga aacccgcgat tctgtaaacc agaaccagca agaatcggaa
    18961 cagaacgacc agaaagcgga acaaaacagc ccaaatacgc aacaaaacga gccagaaacg
    19021 aaacaacctg agccagtagc gcaacaggaa ccggaaaaag tctgcaccgc ctgcggtcaa
    19081 agcggtggcg gcaactgccc tgattgtggc gcggtgatgg gtgacgcaac ataccaggaa
    19141 acattcgatg aagagaatca ggttgaagtt caggaaaatg atccgaagga aatggaaggc
    19201 gctgaacatc cacacaagga gaatgctggt agcgctcagg atcacgccag cgatagtgaa
    19261 actggcgaga cggcagatcc cttaattgcg atgaacggtc atcacgttat cacatccacc
    19321 agcaggacgt gtgaccatct aatgatcgac cttgaaacca tgggaaaaaa tcctgatgcc
    19381 ccgattatct caataggtgc aatatttttc gatccgcaaa ccggagatat gggaccggaa
    19441 tttagtaaga ctatcgatct ggaaactgct ggcggagtca ttgatcgtga cgtcattaaa
    19501 aggtggctga agcaatcacg tgaagcgcag tctgccatta tgaccgatga aatcccgtta
    19561 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
    19621 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
    19681 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
    19741 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
    19801 aatgcacttg atgacgcccg ttaccaggca aaatacattt cagttatctg gcaaaaactg
    19861 atcccgaatc aggctgattt ttaatgttca accccggtcg ttgcccacca gctatagtgg
    19921 cggcgaccat gattagcgaa cgacgctcat ggcaagactt attctgctca ctgagtgggc
    19981 aaaagaggaa ttcagtgaac cggtccctac tccgagtacg ttaagtaaat acgctaaagc
    20041 cggaatgata tttcctctcc ccaaaaaagt tggaagacgc tggcgagtgg atccgcaagc
    20101 tcgctttgtc ggaatggtaa acaagccgga ggtgatcgcc acagatcacc ctgctttgaa
    20161 gaggatactg gaagatggcg cgcccgcgaa aatataaaac cgatgttccg ggattatctc
    20221 cgtattttga caaaagaaat aacaaagttt actggcgtta caggcatccc ataacaggca
    20281 aaaatcacgg tctcggcagt attgaccaga aactggcaga aactattgca gcagaagcga
    20341 acagccgtct tgcccggcag caaatggaac aaatgctcag tctgcaggag aaaattatta
    20401 gtgataccgg cggttcatca actgttacca tttttctgaa taattacaga aaaattcaac
    20461 aggaaagata tgaaaacggc gagatcaaac tcaacacgct gaaacagaaa gcggcccctc
    20521 tcagggtatt tgatgaacgt tttggcacca gacggttaga tgccataacc gtaaaagatg
    20581 tggtatcagt actggaagag tacaaggcca gaggacataa cagaatggga caaattttca
    20641 ggaaagtact gatcgatgtt ttccgggaag ctcagcaaac gggcgatgtc ccgccaggct
    20701 ttaaccctgc agaatcggca aaaaaaccgc aggtgcggat atcaagacag cgactgactt
    20761 ttgatgagtg gatgatgatt tataacgcag cggaaaagga tggttacttt ttacagcgcg
    20821 gtatgctgct ggcactgatg acaggccagc gcctttcaga tatttgcaaa atgcaatttt
    20881 cggatatccg ggatggttat cttcatgtcg aacagcaaaa aacaggaacc cggattgcca
    20941 tccctctggc tctgcgttgc gataaattaa atctcaccct ggatgatgtg gtgtcatcct
    21001 gccgcgattg cgttcttagt ccgtggctat tgcaccacca tcacgcgaaa gggacagcta
    21061 agcgcggcgg gatggttaag ccagcaacat taaccgttgc atttaaaaaa gcccgggatt
    21121 ctgtggatta caactggcgt gctaatggca cccctccctc tttccatgag cagagatctt
    21181 tatcagagcg attgttcaga gagcaggggg ttgataccaa aattttgcta ggccattcga
    21241 atcaaaaaat gaccgatatt tacaacgacg cacgcggtaa ggaatggaaa aaactggtca
    21301 tttgatgacc agttttgcag aggggttttg cagaggtttt gcagagaaaa ttaaaaacga
    21361 tatcctgcgg agaacataaa cacccacgga tccagtcgta ccgagtcttt tacggtagta
    21421 acaccagatt tgtacttagc cgtggtatcg atatccatgt accagactga catgttaacc
    21481 aaccagtcac ggttaatcag ataatcaacc cccacctgcc cggcagctcc ccaggaatct
    21541 ttcagactga gatcggaaag ccctgcctct ttgccatgat cgttaaatcc attatcaaag
    21601 aaggtggtgt agttaatacc tgccccaacg taaggacgga atttgctgct ggcatcacca
    21661 aaataccact gcgccatcag tgttggcggc agatggtgaa cggttgcaat atcgccggtc
    21721 gcccgggtgc cgattttatg gcggaacggc gtcgctgcca gtaattccac accaatgttg
    21781 tcggtcgcca tataagtaaa cgtcaagccc agttgagtgt tattggtcac gctgaatcca
    21841 cccagacttc ctaacgtacc accagcacct tctgttggac gtacggttgc agaacctgca
    21901 cgcataaaaa attcgcctgc ttcatgcgca aaggcactgc cagagagaag agttgttact
    21961 gccaaagccg ccactgttaa ctttttcata tccgctccgt cgttatggtt ataaaaacgt
    22021 gacgaatata cctatattgg agtaacaaat gatcctacat agatcacatt acatatgata
    22081 attcaccctt tattgatctg gattaattta gaagttgcat tgagaatcct ggttaacgtg
    22141 atttaaatca atttttaaat attttcgcta cgcaaatatt aactcgatcg ccacttgccg
    22201 cggtttgccc acgcctgaag gccagcaatt acctccgctt tacaaagata tgcttttcca
    22261 tacaagacct gatgctgcca cccgaggctt aaccagggta caattgcccg ctaattaaca
    22321 cctgcataaa actcaaggag agtgcatgtc tatcacggcg cagtccgtat accgtgacac
    22381 cggaaatttc ttccgtaatc aatttatgac cattctgttg gtatcgttgc tatgtgcgtt
    22441 tatcacagtg gtgttagggc atgttttctc acccagtgat gcacagcttg cgcagctcaa
    22501 tgacagcgtg cccgttagcg gcagtagtgg gttgttcgac ctggttcaga atatgtcacc
    22561 ggaacagcaa caaattttgc tgcaggcttc agcggcgtcc actttttcag gattaatcgg
    22621 taacgccatt ctcgccggag gcgtaatatt aattatccag ctggtgtctg cgggtcagag
    22681 agtcagtgcg ctgcgagcta ttggtgccag tgcaccgata ttgccaaagt tatttattct
    22741 gatttttctg actacccttt tagtacagat tggcatcatg ctagtggtcg ttccgggaat
    22801 tatcatggcc attttactgg ccctggcacc ggtgatgctg gtacaggaca aaatgggcat
    22861 ttttgcctcg atgcgtagca gtatgcggct gacttgggcg aatatgcgtc tggtggcacc
    22921 cgcagtactg agctggttgc tggcaaaaac actgttgctg ctttttgcct cttcttttgc
    22981 cgcattaacc ccggaaattg gtgccgtact ggcgaacacc ttgagcaacc tgatttcagc
    23041 cgtattgctc atctatctgt tccgtctgta tatgttgatt cgccaataac cttaagcatt
    23101 tgattacgga atcgtaaaat gaagcagttt cttgattttt taccgctggt tgtctttttc
    23161 gcgttttaca agatttatga catctatgcg gctactgcgg cgctgatcgt cgccacggcg
    23221 attgtgctta tctatagctg ggttcgcttt cgtaaggttg agaagatggc cctgatcact
    23281 tttgttctgg tggtcgtctt cggtggcttg acgctgttct tccacaatga tgagtttatt
    23341 aaatggaagg ttacagtcat ttatgctctg tttgcgggag ccctgttagt cagccaatgg
    23401 gtgatgaaaa agccgctaat tcagcggatg ctgggtaaag aactcacgct gccgcaaccg
    23461 gtatggtcga agctgaatct ggcctgggct gttttcttta tcctttgcgg tctggcaaac
    23521 atctacatcg cattctggct gccgcaaaat atttgggtca actttaaagt ctttggactg
    23581 accgccctta ccttaatctt cacattgtta agcggtatct atatctaccg ccacatgccg
    23641 caggaagata aatcctaacc agactgccag tccttcgggg ctggcgctgt atctcctctg
    23701 ttctcctcag gaaaatcata gtagcatcgc gcctgtgatt ttccttttaa gtcggtttta
    23761 ccatgtctac aacacataac gtccctcagg gcgatcttgt tttacgtact ttagccatgc
    23821 ccgccgatac caatgccaat ggtgacatct ttggtggttg gttaatgtca caaatggata
    23881 ttggcggcgc tattctggcg aaagaaattg cccacggtcg cgtagtgacc gtgcgggttg
    23941 aaggaatgac tttcttacgg ccggttgcgg tcggcgatgt ggtgtgctgc tatgcacgct
    24001 gtgtccagaa agggacgaca tcggtcagca ttaatattga agtgtgggtg aaaaaagtag
    24061 cgtctgaacc aatcgggcaa cgctataaag cgacagaagc attatttaag tatgtcgcgg
    24121 ttgatcctga aggaaaacct cgcgccttac ctgttgagta atagtcaaaa gcctccggtc
    24181 ggaggctttt gactttctgc ttactgaatt tcggtggtgc cgttaatttt aaacaggata
    24241 ttcaccacaa tcccactgcc tggcttaccc ggctcataac gccatctgcg catcgcattt
    24301 ttcacctcac gctcaaacat gttcgcaggc ttggctgaga ggatttgtac gttatccacg
    24361 cgaccatccg gcgtgacgtc aaatttaact ttaacctgcc cttcaatgcg caatgcctgt
    24421 gctcgtgccg gatactgcgg ctgattacgg cttaatgcgc gtggtcctga agccacactg
    24481 gtaaccggct tgctggttgc agccgttgct gtacttgatg tcaggcgtgc cagtgccgta
    24541 ttttcaaacg gtgatgccgg acgcgactct acgggtttga catcgcgttt tggctgctcc
    24601 tgtacctttt tcaccggctt cggttttggc ttaggtttcg gcttcggctt ttcaatgacc
    24661 accggtgctt cttttggcgg ttcggggatc ggctcaggtt ccggttctgg ctctaccacc
    24721 ggctccggtg gcggctgaac ggcttgtggc ggttcgagat cagcaggcgc aaccatcgtg
    24781 acagaaattg gctgcgcagg cgcaggtagt tcaataacct gatgtaccga ggtatagagc
    24841 agacccgcca caacagcacc atgaatgcag accgaaagta acgtcggcca ggggaagcgg
    24901 cgaggtaaat caagggtcat tgaagtcata atcatttcag tagaaaaacc aagcctcgat
    24961 tttaaatgca aatagcaatc atattcaata aggcaagacg atcaggatca gtgaaaatgt
    25021 catttttgcc gtaattaacc ttactttatg catggtctta acaaagacat catctttccg
    25081 ttgcagatct tcgccctttc aaataacgta ctttacaact ttcccgaaca aggagttgtg
    25141 cccgtgttgt atgttattta cgctcaagac aaagctgata gcctcgaaaa acgcctttcc
    25201 gttcgtccgg cacatttagc acgtttacag ttactgcatg atgaaggtcg tttgttgaca
    25261 gctggtccaa tgccagcggt agacagtaac gatccagggg ctgcgggatt tactggctca
    25321 accgttattg ctgaatttga atcgctggaa gcagctcagg cctgggccga tgctgacccg
    25381 tacgtagcgg caggagtcta tgaacacgtt tcagtgaaac catttaagaa agtgttctga
    25441 atagagattc aatatttaag ggcacggttt ttgcaaccgt gttcttgttt ggcctttcct
    25501 tatagccggt cagtgcattc accaccacaa gcagataata actttatctg tatttcttta
    25561 tatgatgtga gcatattcag gaaaaatgac ctttcctgag gtacagttgc tcgctcaaaa
    25621 cctttgatta ctgcggataa agtcatctac tattgtttaa cacattcacg cgctgcatgt
    25681 atgttcagaa aatgtgcacg ctaagataat taaacaaaat tgtggttaca gaggtatcag
    25741 tgagtcactg ggctacattc aaacaaacag caacaaattt atgggtgacc ctacggcacg
    25801 acattctcgc gctggccgtc tttttaaatg gattgcttat ttttaaaaca atctatggta
    25861 tgtcggtcaa tttgctagat attttccata tcaaagcatt ttcagagttg gatctttcct
    25921 tgctggcaaa cgccccacta tttatgctcg gcgtctttct tgtcctgaac tccattggct
    25981 tactgttccg ggcaaagctc gcatgggcaa tcagtatcat tttgttgttg atagcgctaa
    26041 tttataccct gcatttttat ccctggttga aatttagtat tggattttgc atttttacgc
    26101 tggtgttttt gctgatactg cgcaaagact tctcccacag tagcgccgca gccgggacaa
    26161 tttttgcatt tattagtttc acgacattac tgttttactc aacctacggt gcgctttatt
    26221 taagcgaagg ttttaatccg cgaatagaaa gtttgatgac cgcgttctat ttttcgatag
    26281 aaaccatgtc aaccgtcggc tatggcgata ttgtccctgt ttctgaatca gcacgattgt
    26341 tcactatttc ggtcattatt tccggtatta ccgtttttgc cacatctatg acctcaattt
    26401 ttggtccgct tatccgcggg ggattcaaca aacttgtaaa aggaaacaat catacaatgc
    26461 atcgtaaaga tcattttatt gtttgcggac attcgattct cgccatcaat acgattctgc
    26521 aactgaatca acgcggacaa aacgtaacgg ttatcagcaa cttgcctgaa gatgatatca
    26581 agcaacttga gcaacgctta ggcgataacg ctgatgttat ccccggtgac agtaatgaca
    26641 gttcagtatt aaagaaagcg ggaatcgatc gttgcctggc cattctggcg ctgagtgata
    26701 acgatgcaga taacgcgttt gttgtactct cggcaaaaga tatgagcagt gatgtcaaaa
    26761 cagttctcgc cgtcagtgat agcaaaaacc tgaataagat taagatggta catccggata
    26821 tcattctctc accgcaactg tttggcagcg aaattctggc gcgagtttta aatggtgaag
    26881 agattaataa tgatatgctc gtttcaatgt tgttgaactc cggtcatggc attttcagcg
    26941 ataacgatga acaagaaacg aaagctgaca gtaaagaatc ggcgcaaaaa tagagtttaa
    27001 agatggagga gtaaacttat tgggcaccgc aatggtgccc agtacattta gatattgtag
    27061 atcgcaaaca ataacgagtt acgctgtttg tttagaatgc acttcctgat gctgtggata
    27121 cgcatatttc ggcgcgattg cccctcaagc caacgcgatt tacgtcggct agcctgacgc
    27181 tgcatgcgcc agcgccccac ttccgttcta ctccgcttca tgttaactac tctatgcaat
    27241 aacagaatgg tcattatacc cccgcccccg acgctgacca gtgcttttcc cgcgttttta
    27301 tttgccagat gaatccatat gcgtaaactc ataacaatgc gctttcaaaa ggatttctaa
    27361 tctatgacaa ccgtttatac gttggtgagt tggttggcca ttctgggata ctggttgctc
    27421 attgcaggcg taactttacg cattctaatg aaacgacgcg cagttccctc cgcgatggcc
    27481 tggctgttga ttatttacat tctgccgtta gtcggaatta ttgcctatct tgccgttggc
    27541 gagctccatt taggcaaacg ccgcgctgag cgcgccagag cgatgtggcc ttccaccgca
    27601 aaatggctta acgaccttaa agcctgtaag catatcttcg ccgaagaaaa tagcagtgtc
    27661 gctgcgccat tattcaagct ttgcgagcgt cgtcagggga tcgctggggt caaagggaat
    27721 cagctacaac tgatgaccga gtcagatgat gtgatgcagg cgttaatccg cgacatccag
    27781 ctcgcgcgcc ataatattga gattgtgttt tatatctggc agcccggcgg catggcggac
    27841 caggtggctg aatcattaat ggccgctgcg cgacgcggca ttcattgccg attgatgctc
    27901 gactccgccg ggagtgtggc ttttttccgc agcccgtggc ccgagctaat gcgtaatgcc
    27961 ggtattgaag tggtcgaagc cttaaaggtc aatctgatgc gtgtgttttt acgccgtatg
    28021 gacctgcgcc aacatcgcaa gatgatcatg atcgataatt acatcgcgta caccggcagc
    28081 atgaatatgg tcgatcctcg ctacttcaaa caagatgcgg gcgtagggca atggattgat
    28141 ctgatggcgc gtatggaagg ccccatcgcc accgcgatgg ggattattta ttcctgcgac
    28201 tgggagattg aaaccggaaa acgtattctg ccgccaccac cagatgtcaa tattatgccg
    28261 tttgaacagg ccagcggtca caccattcac acaattgctt ctggccccgg ctttccggaa
    28321 gatctcattc accaggcatt attgactgcg gcttattcgg cgcgtgaata tttgatcatg
    28381 accacgccct actttgtgcc aagcgatgat ttacttcatg cgatttgcac ggcggcacag
    28441 cgcggggtgg atgtcagtat tatccttccg cgaaaaaatg actcgatgct ggtcggctgg
    28501 gccagtcgcg cattctttac ggaacttctt gctgctgggg ttaaaattta tcagtttgaa
    28561 ggcgggttac tgcataccaa gagcgtgctg gtcgatggcg aactaagtct ggttggcacc
    28621 gttaaccttg atatgcgtag tctgtggctg aatttcgaga ttaccctggc aatcgacgat
    28681 aaaggttttg gtgcagacct tgccgccgtt caggacgatt atatttcgcg ttcacgtctg
    28741 ctcgatgccc gtttatggct aaaacgtcca ttatggcaac gtgtcgccga gcgactgttt
    28801 tacttcttca gcccgttgct gtaaaacgtc tccaacagag gttaaacagg tagtgactat
    28861 ggatatggat ctaaacaatc gcctgactga agatgaaacg cttgagcagg cctacgatat
    28921 ttttctcgaa ctggctgccg acaatctcga cccagctgat gttctgctgt tcaatcttca
    28981 gtttgaagag cgcggcggcg cggagttatt cgatccggtg gaagactggc aggaacatgt
    29041 tgatttcgat ttgaacccgg acttttttgc cgaagtggtg attggtctgg cggacagtga
    29101 ggatggtgaa atcaacgatg ttttcgcccg cattctgtta tgccgcgaaa aagatcacaa
    29161 actttgccat atcatctggc gggaataaat aatcgctttc ggcgtgaaaa agggctgaca
    29221 actgtcagcc cttattgtgt ttaaagcgga tcgactttca ggcaagaaac ggcgtgtctg
    29281 aaactccctt ccagcacagg acgtgttttg gcgcactccg gaccggcaat cgggcaacgg
    29341 gtacggaaaa cacaaccgga aggcgggttg atcggcgacg gtaattcccc ttccagtaac
    29401 tggatggttt tgttcttctc cagatccgga tcaggtatgg ggactgccga catcaatgcc
    29461 ctggtgtaag gatgtagtgg attgtggtag acctcatcat aggtccccag ttctaccgca
    29521 tggccgagat acatcaccaa cacacgatcg gaaatgtgtt ttaccacggc caggtcatga
    29581 gcaataaaaa ttaatgacaa tcccatctca cgttgcagct gctggagcag gttgaccacc
    29641 tgcgcctgaa ttgacacgtc cagcgccgac accggctcat cacagataat cagcttcggt
    29701 tcaagaataa gagcacgagc aatcccgata cgctggcact gcccaccaga gaactcatgc
    29761 ggatagcggt taatcaggtt aggcaataac ccgactttca gcatcatcgc cttcacgcgc
    29821 tcgcgaactt cctgacgtga cattttcgga tgataagtac gcagtggttc agcgatgatc
    29881 tcgccgatgg tcatgcgcgg gtttagcgat gccaacggat cctggaaaat catctgaata
    29941 tcactgcgaa cggcacgcca ttcatcgggc ttaatgccca gcaactcttt acctaaccag
    30001 gcaacatgac cgtcggtcgc tttaaccagg ccaatgatgg cgcgagcaaa ggtggactta
    30061 ccgcatcccg attcccctac cacacctaat gtttcccctt catacaggcg aagcgtgaca
    30121 ccatcgacgg ctttgagcgt tttcggcggt tgccagaacc actgtttgcc atctttgatt
    30181 tcaaagtgca ctttcagatc ggcgatttca aggaggactt ttcttccttc ggttacagca
    30241 ttcataacag ttcctccacc ggtttaaagc aagcacacag acggccaagc gtaaactctt
    30301 ccagcggcgg tgcgctacta caaatttcca tcgcatgcgg acaacgtggc tggaacgggc
    30361 aacctttcgg taatcgcagc aggtttggcg gattacccgg gatggtcaac attgtttcac
    30421 cttccgcatc gagacgcggc accgcgttga gcaaaccgat agaataagga tgaacgggtt
    30481 gataaaagac atcgcgcgcg ttgccatatt ccatcgtgcg cccggcgtac attaccagca
    30541 ctttgtcaca gatccccgcc accacgccaa gatcgtgggt aatcataatg atggcggtat
    30601 taaattcccg tttcagttca tttaatagcg tcatgatctg cgcctgtacg gtgacgtcca
    30661 gcgctgtagt gggttcatcc gcaatcagca gcttaggtcg acatagcaat gccatcgcaa
    30721 tcataactcg ctgacgcatg ccgccagaaa actcgtgcgg gtacattttc atgcgtttac
    30781 gtgcttccgg catttttacc gcatcgagca tccgcaccga ctcttcaaac gcctcagctt
    30841 tgctcatgtt cttatgcagc atcagcactt ccatcaactg ctcaccgacg cgcatatagg
    30901 gattcaacga agtcattggg tcctgaaaaa tcattgagat ttgttcagcg cgcagtttat
    30961 tgagttcatg ctctggcaaa ttgaggattt cacgcccatt gaaggttgcc gatccgccaa
    31021 tacgtccgtt ggcagccaac aggcccatca acgcaaatgc agtttgcgat ttacccgaac
    31081 cagactcacc tacaataccc agcgtttctc cggcacgtag ggaaaaattc aaatcattga
    31141 ccgtcgtgac gtcgccgtcc ggggtactaa aggtgacacg caaatctttc acgttcagca
    31201 gtgcgtcagc ctgttgttgt gcgagcggca cagttgcagt ttcaattacg ctcatggctg
    31261 cactccttaa cgatctttcg ggtcgagggc atcacgcaag ccatcgccga taaagttgaa
    31321 acaaaacagc gtcaccacga ggaatcccgc tgggaacaac aataaccatg gagagacttc
    31381 catcgagttc gcgccatcac tcagcaatgc accccagctg cttaacggct cttgcgtacc
    31441 caaccccagg aagctaagga aagattcaaa gaggatcatg ctgggcacca gaagtgatgc
    31501 gtagaccacc accacaccga gtacattcgg cacaatgtgg cgaataacaa tgcccgatgt
    31561 cgatacaccg ccaacttgtg ccgcctcaat aaactctttg cgcttcagac tcagggtttg
    31621 cccacgcaca atacgagcca tatccagcca ggaaaccatg cctatcgcca cgaaaatcag
    31681 caggatgttt tgaccgaaaa aggtcaccag caaaatgacg aagaacatga atgggaagga
    31741 gttgaggatt tccagcagac gcatcattac cgaatccact ttaccgccca gataaccgga
    31801 aagcgaacca taaagtgtcc cgacgaccac agccaccagt gccgcagcaa caccgaccat
    31861 gagtgagata cgcccaccaa tcgcaacgcg cacaagcagg tcgcgaccgg atgagtcagt
    31921 accaaagtag tgaccggact ccatatccgg ggcgctggac atcatcgccc agtcagtatc
    31981 gtcataggca aactgggaaa gcatcggtgc caggattaca aataacgcga tcagcaccag
    32041 caccatcaga ctggcaaccg ccgcacggtt atgaataaaa cgtcgacgtg cgtcctgcca
    32101 caagctgcgc ccttcgacct ccagcttttc actgaaattt tccagcgtct cgctgttttt
    32161 cttacttaac atcatcgcga gctccagtat cagtaacgga ttttcgggtc gataaccgca
    32221 tatagcacat cgacaatggc attaaacaaa atggttaaag caccaaccag gatggtcagg
    32281 cttaacacta aggaatagtc acggttcaat gcgccattaa cgaacagttg tccaatcccc
    32341 ggcaaaccat aaatggtttc gataaccata gaaccggtaa taatgccgac aaatgcgggg
    32401 cccatataag agagcacggg taacagagca ggttttaatg cgtgacgtaa aatgatccgc
    32461 cgcataggta accctttcgc ccgggcagta cgaataaaat tggagtgtaa tacttcaatc
    32521 atagagccac gggtaatacg cgcaatactg gcgatataag cgagtgacaa cgccaccatc
    32581 ggcaatatca tgaatttaag cgccccacca ttccagccac cgcccggcag ccaatgcaaa
    32641 atgatcgcaa atatcatgac taataatggt gcaaccacaa aactggggat aacaaccccg
    32701 gtcattgcca gccccatcac ggtatagtcc catttggtgt tttgttttaa tgcggcaata
    32761 acgccagcac taacacccag tattaccgca aggaaaaatg ctgcggctcc cagtttggca
    32821 gaaacgggaa aactggatgc aaccaagtca ttgaccgaat aatctttata tttaaacgat
    32881 ggaccgaaat caccgtgcgc cagttgtttc aggtagctga aatactgtgt catgatcgga
    32941 tcattaagat gatatttcgc ttcgatattg gccatcactt ctggcggtaa agtacgttcg
    33001 ccggtaaaag ggcttcccgg cgcgaggcgc atcataaaga acgaaatagt aataagaata
    33061 aatagcgtcg gaatcgcttc cagacagcga cgtagaataa attttaacat tgccctacct
    33121 tctggcctgt gccttctgta atgcgataaa aatcagacac cgtggagcag gacactcctg
    33181 ccccacgtat tgccattagt gcttcacaat gtacatattc cgggtatagg tattatccag
    33241 cggatctttg ccggtatagc caccaaccca cggtttcacc agacgcgcat tcacgtagta
    33301 ataaacagga acaatggccg aatccttatc cagctgttgt tctgctttag tgtacagagc
    33361 tgtgcgctgc gcctcgtcag tcactttcag cgtttccgcc ataatgctgt caaaggccgg
    33421 gctcttataa tgcgcggtat tcatcgagct gttcgaaagc atggtgttca ggaaggaagt
    33481 tggttcgttg tagtcagcac accagcctgc acgggccaca tcaaaagtac cctggtgacg
    33541 ggtgtcgagg aacgttttcc actcctggtt aaccagtttg acgtttacac caatgttttt
    33601 cttccacaat gaagaggcag caatcgccag ctttttatgc agatcggagg tgttatacaa
    33661 caggttgatt gtcaacggtt tgtctgcggt ataacccgct tcagccagca gttttttcgc
    33721 ttcttcgtta cgtttttcct ggctccagcc aaaccattcc ggctgagtca attttgcgcc
    33781 atcagtatac ggtggagtgt aaccataggc gggcatgtcg ccctgcgctt tcactttatt
    33841 aacaatgata tcgcggtcca tacctagttt cagtgcggta cgcacacgca catcgttgaa
    33901 tggcggtttc tggttgttaa tttcgtaata gtaagtgcac aggtatggat caacgtgaac
    33961 ttcgtccggg atctcttttt tcagcttctg gaacaattcg atcggcatgt tgttataagt
    34021 catgtcgatt tcaccactac ggtagcggtt gacatcggta acttcagaag caataggcaa
    34081 ataggttacc tggttaataa cggttttcgc gttgttccag taggtcgggc tgcgttcaag
    34141 aacgattcgt tcgtttacga cccaatcttt taaggtatag gcaccgttgg tgacgatatt
    34201 accaggctgg gtccattttt cgccgaattt ctcgatagcg gcttttggca ccggtgaagt
    34261 tgatgggtga acaagtaatt tatagaagta cggaacgggt tcacttaagg tgacttctaa
    34321 tgtgtgatca tcaatagctt tcacaccgag atcggtaatc ggttttttcc cttcaagaat
    34381 ttcatcaata ccggcgatat gcccatattg cagataactg gcatacggag aagcagtgtt
    34441 cggatcaaca gaacgttgcc agctatacac aaagtcttgt gctgtgactg gcgtaccatc
    34501 agaccatttc gcatctttac gcaaatggaa ggtccagact ttcgcgtctt tattatccca
    34561 ggattcagcg acgccaggtg ccggatgacc gtcaagatcg ctgaccagta agccttcaaa
    34621 caggtctcgg ctgatattag actccggaac accttcaatt ttgtgcggat ctaatgactg
    34681 aacttctgaa ccattgttac gtaccagcgt ttgtttttcc gccagtgtga cgcctgcggg
    34741 tacatcagct gccagcgcga cattccctgc cattagcgca gccagaacgc cagctgctac
    34801 taaacttctc ttggtgatgt tggtcattgt tttttggact ccctcattat aattactggt
    34861 attaaccagc atgtgtaatc ccggattggg accctgcaca ggctcggaga ctttctgctg
    34921 tcaggttact ttatcgttac ggattgctat caccgacttt atttattctg gtgattcaga
    34981 cgatcacctt atgtcgattc gtactgtgtc tcccctgtcg agacacatta ttctttcgcg
    35041 acgctcttta attgttaatt gttaattatt ctcattacca ataaagaatc gtctggcggt
    35101 tgcccggaaa gtactaaatg cggttcttac tcgccaatac attttgcaaa attgttaagc
    35161 aattctcttt tattgaccgt atcagaatct atagcttgca tccatgcctt aacaaacatc
    35221 tttcaattca atcaaataga gattggctaa gcatgtctat cttttctgat tacagtttca
    35281 gtattgaaat gcacaataat ttaacaattg attattattt agcactttcc tctaccaaag
    35341 gcagtggaat tactaatatc atttcaatta ttcttcagca agcccaggat tatgatgtgg
    35401 cgaaaataac atgatggtga ggagaagcag ttaagaaaat ccttataact aactgtaatt
    35461 tgagaagaat atttttacct attccttttg cttttgataa ctgcgcgggg gtaatgactc
    35521 caacttactg atagtgtttt atgttcagat aatgcccgat gaccttgtca tgcagctcca
    35581 ccgattttga gaac
//
LOCUS       DAJTSL010000023        35520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTSL010000023 DAJTSL010000000
VERSION     DAJTSL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.023"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            108..455
                     /gene="grxD"
                     /locus_tag="OO433_001007"
     CDS             108..455
                     /gene="grxD"
                     /locus_tag="OO433_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6049565.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(506..4913)
                     /locus_tag="OO433_001008"
                     /pseudo
     CDS             complement(506..4913)
                     /locus_tag="OO433_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(5006..5653)
                     /gene="rnt"
                     /locus_tag="OO433_001009"
     CDS             complement(5006..5653)
                     /gene="rnt"
                     /locus_tag="OO433_001009"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6049566.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRSAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMIAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5756..6163)
                     /gene="gloA"
                     /locus_tag="OO433_001010"
     CDS             complement(5756..6163)
                     /gene="gloA"
                     /locus_tag="OO433_001010"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6049567.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFMEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6244..7341)
                     /gene="nemA"
                     /locus_tag="OO433_001011"
                     /pseudo
     CDS             complement(6244..7341)
                     /gene="nemA"
                     /locus_tag="OO433_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            complement(7378..7977)
                     /gene="nemR"
                     /locus_tag="OO433_001012"
     CDS             complement(7378..7977)
                     /gene="nemR"
                     /locus_tag="OO433_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6049568.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYTAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8080..8319
                     /locus_tag="OO433_001013"
     CDS             8080..8319
                     /locus_tag="OO433_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6049569.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8368..9264
                     /locus_tag="OO433_001014"
     CDS             8368..9264
                     /locus_tag="OO433_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6049570.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9345..9866
                     /gene="sodC"
                     /locus_tag="OO433_001015"
     CDS             9345..9866
                     /gene="sodC"
                     /locus_tag="OO433_001015"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6049571.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVITPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9867..11878)
                     /locus_tag="OO433_001016"
                     /pseudo
     CDS             complement(9867..11878)
                     /locus_tag="OO433_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994342.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11878..12735)
                     /locus_tag="OO433_001017"
     CDS             complement(11878..12735)
                     /locus_tag="OO433_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6049572.1"
                     /translation="MSIKTIKYFSIIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(12801..12974)
                     /locus_tag="OO433_001018"
     CDS             complement(12801..12974)
                     /locus_tag="OO433_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6049573.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPL"
     gene            13175..13609
                     /gene="slyA"
                     /locus_tag="OO433_001019"
     CDS             13175..13609
                     /gene="slyA"
                     /locus_tag="OO433_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6049574.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13656..14123)
                     /gene="slyB"
                     /locus_tag="OO433_001020"
     CDS             complement(13656..14123)
                     /gene="slyB"
                     /locus_tag="OO433_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6049575.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14397..15506
                     /gene="anmK"
                     /locus_tag="OO433_001021"
     CDS             14397..15506
                     /gene="anmK"
                     /locus_tag="OO433_001021"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6049576.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VIRPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            15604..15933
                     /gene="mliC"
                     /locus_tag="OO433_001022"
     CDS             15604..15933
                     /gene="mliC"
                     /locus_tag="OO433_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6049577.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15992..16648
                     /gene="pdxH"
                     /locus_tag="OO433_001023"
     CDS             15992..16648
                     /gene="pdxH"
                     /locus_tag="OO433_001023"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6049578.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16777..18051
                     /gene="tyrS"
                     /locus_tag="OO433_001024"
     CDS             16777..18051
                     /gene="tyrS"
                     /locus_tag="OO433_001024"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6049579.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18110..18973
                     /gene="pdxY"
                     /locus_tag="OO433_001025"
     CDS             18110..18973
                     /gene="pdxY"
                     /locus_tag="OO433_001025"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6049580.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(19017..19622)
                     /gene="gstA"
                     /locus_tag="OO433_001026"
     CDS             complement(19017..19622)
                     /gene="gstA"
                     /locus_tag="OO433_001026"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6049581.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNETLKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19728..21230)
                     /gene="dtpA"
                     /locus_tag="OO433_001027"
     CDS             complement(19728..21230)
                     /gene="dtpA"
                     /locus_tag="OO433_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6049582.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(21841..22476)
                     /gene="nth"
                     /locus_tag="OO433_001028"
     CDS             complement(21841..22476)
                     /gene="nth"
                     /locus_tag="OO433_001028"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6049583.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22476..23171)
                     /gene="rsxE"
                     /locus_tag="OO433_001029"
     CDS             complement(22476..23171)
                     /gene="rsxE"
                     /locus_tag="OO433_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6049584.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(23175..23795)
                     /gene="rsxG"
                     /locus_tag="OO433_001030"
     CDS             complement(23175..23795)
                     /gene="rsxG"
                     /locus_tag="OO433_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6049585.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23799..24857)
                     /gene="rsxD"
                     /locus_tag="OO433_001031"
     CDS             complement(23799..24857)
                     /gene="rsxD"
                     /locus_tag="OO433_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6049586.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGADMNTLHLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24858..27080)
                     /gene="rsxC"
                     /locus_tag="OO433_001032"
     CDS             complement(24858..27080)
                     /gene="rsxC"
                     /locus_tag="OO433_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6049587.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDARFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAIE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            complement(27073..27651)
                     /gene="rsxB"
                     /locus_tag="OO433_001033"
     CDS             complement(27073..27651)
                     /gene="rsxB"
                     /locus_tag="OO433_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6049588.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVELHA"
     gene            complement(27651..28232)
                     /gene="rsxA"
                     /locus_tag="OO433_001034"
     CDS             complement(27651..28232)
                     /gene="rsxA"
                     /locus_tag="OO433_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6049589.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFTAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(28309..28749)
                     /locus_tag="OO433_001035"
     CDS             complement(28309..28749)
                     /locus_tag="OO433_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6049590.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVSYFKRSSRVKATFVNP"
     gene            complement(28835..29050)
                     /gene="ydgT"
                     /locus_tag="OO433_001036"
     CDS             complement(28835..29050)
                     /gene="ydgT"
                     /locus_tag="OO433_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6049591.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(29323..29448)
                     /gene="blr"
                     /locus_tag="OO433_001037"
     CDS             complement(29323..29448)
                     /gene="blr"
                     /locus_tag="OO433_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6049592.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            29661..30701
                     /locus_tag="OO433_001038"
     CDS             29661..30701
                     /locus_tag="OO433_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6049593.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(30736..31737)
                     /gene="add"
                     /locus_tag="OO433_001039"
     CDS             complement(30736..31737)
                     /gene="add"
                     /locus_tag="OO433_001039"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6049594.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRAGIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(31840..33012)
                     /gene="malY"
                     /locus_tag="OO433_001040"
     CDS             complement(31840..33012)
                     /gene="malY"
                     /locus_tag="OO433_001040"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6049595.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(33022..34614)
                     /gene="malX"
                     /locus_tag="OO433_001041"
     CDS             complement(33022..34614)
                     /gene="malX"
                     /locus_tag="OO433_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6049596.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQTQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPAAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYAIFRFAITRFNLKTPGRDSEVASSIEKAVAAAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            34789..>35520
                     /gene="malI"
                     /locus_tag="OO433_001042"
     CDS             34789..>35520
                     /gene="malI"
                     /locus_tag="OO433_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6049597.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTI"
BASE COUNT         8899 a         9137 c         9247 g         8237 t
ORIGIN      
        1 aaagggataa aatagcaaaa gcgtgatgaa aatgttattt tccgatgccg cactgacccg
       61 ctacaatgtc agattactgc cgtaaagaag tttgaggaag caagacaatg agcaccacta
      121 tcgaaaaaat ccaacgccag attgctgaaa acccgatcct gctgtacatg aaaggttcac
      181 cgaaactgcc gagctgcggt ttctctgccc aggcagtcca ggcgcttgcc gcatgtggcg
      241 aacgttttgc ctatgttgat attctgcaga atccggacat tcgtgcggaa ctgccgaaat
      301 atgctaactg gccgaccttc ccgcaactgt gggttgacgg cgagctggtc ggcggttgtg
      361 atatcgtgat cgaaatgtat cagcgtggcg aactgcagca gctgatcaaa gaaactgccg
      421 ctaaatacaa gtctgaagag ccggacgcgg aataatccgt cctgcaatac tgacggatgc
      481 gaacgggcat ccgtcagtcc tttctctatc cccaatccag cccttgtggc gaacttgaaa
      541 atcccgcctc acgcagcagc gtaaacatcg gcgtttgccg gaccggtaga tcattcactt
      601 ctgttagcgt aaagcgcagc cgtggttcgc gacgcagtgc ggtagtcagc gcgtggaaaa
      661 cctctggggc ttttcctgcc agaccagcat ttttttgcca ccttgcgcca gatagagtaa
      721 caatttgccg ctagaaacga ccaccagcgc acccgcccga cgcgttggaa ccagcgttgc
      781 cggatgtgca ggccagggca gaagatttcc ccacacatta gccggatcgt tggcagagag
      841 cgccactggt gtatagtggc gcgtttgcgt ggcttgtgtc gccagatcgc gccatcggtc
      901 aatagtcaga cgttcagcga attgcgcgcc acccagacct tctacaaaac gacctcgcat
      961 aattcgcccg gagtcttcca tacttcgaca aagcgtttgc atcgatggaa acccgccagg
     1021 gatattttcg gctatcaccg cctgacgact gatgatgccg tagcggtcga gcatattttc
     1081 cgccagcgcc agcatccttt cggtatcgtt tagtggctcc acctgcaata acgaccagcg
     1141 tccagccaga tttggtgtgt tgtaagatac cagcggcgag acgggttgcg catagacagg
     1201 acgtccacga cgagcccggt gactgcggcg agttgaggtg cgtgcgttgg aactgctgcg
     1261 ggtgagggcg cgtaacggtg cccaaatgtc gctggtgatg acgccttgcc agaccagcgc
     1321 ccataacgcc tcttgcaggg cagagagatc aaccgattcg ccgattttgt cgcgtatccg
     1381 ttggctgatt tgttgtgcaa accaggctcc tccgtcagcc agaacagcga ctatcgcttg
     1441 ttgcagcgcc gaacgattcg cctgatccgc ttcggcagga gtgaacgatt ctgcagcata
     1501 ttcctgtaga tgcagtgcca ccaggccgtc atcttcaccc agtttttttt gccccgacca
     1561 gataaccgca ccggttgcca gtaattcatc gagcatttct gacgaatagt cgcgtacgcg
     1621 ggcaggcagg atctggcttt cccagagtga ggcgggtaaa ccgactccgg caagctgttc
     1681 gatcacccgc atcacgccat ctacgccctc ataaacgcct ggcgatgttg aggcaaagag
     1741 cgccgggcta ccatcggtgg cgggtaatac gccctgacgt tccagcagca atcgcgcata
     1801 ggtcgtggct gcaacgggac gcgtcgcttc tctggcggct tgcagcgagc gcaaacgcag
     1861 acgacgaaat acttcatcgc tcacccagat gtcttgttgc agattcatca ccagaccctg
     1921 ttcacgcagt tgctgaagct gctcttcgac aatggcaata ccgagactaa actcatgagc
     1981 cagttgttca gccgtgacca gagcatgagc ccggagatag cgcagaaaga ggtcgcgaag
     2041 cgggtaactg actctatgta aataaatctc tggcaatgac tctggtagtc gtacgccgag
     2101 ggcatcacgc agcctggcgg catcatccat acaggcaaga cgctcctgtc cgctaatcat
     2161 cgctgggaag attcgtttta ctgcaagaag attttccaga tacgacgcaa cctcttcact
     2221 gctgcctgta tgccgttgcg ccaggtcttc aacggtcatt ggccccagtt cgcgcagcag
     2281 gtcgaacaat ccttcttcac ctttcgctct tctgccagga gccagtcgtt gcaactcttc
     2341 ttccacctgg cgaatgacct gcgggtcgag taattccccc ggatcgacct gtccgagtag
     2401 attgcgcagt aactcgctgt ccagcgacag tacggatgcc cggcgctctg ccagcggggc
     2461 gtcgctctgg tacataaatt ccgcgacata gccgaacaat aaacttgtgg cgaaaggcga
     2521 gggcgtagtg gtcgttacat cggatatttg aatttcgcca ccgttcaggc gacgcatcaa
     2581 acgttccagt gcgggaagat cataaacatc ttgcagacat tcgcgtagga tttcgagaat
     2641 gaccggaaaa tctggatatc cctgagcgat ttccagcaac tgactggcgc gcagccgttg
     2701 ttgccaaagc ggggtgcgat ggcccggagt gcgccccggc attaataatg cccgcgcggc
     2761 gcattcgcga aaacgggcgg cgaaaagtgc cgagctgcct accgcctcgc ggacaatttg
     2821 cagcaacttt tctggttcaa acaaaaaaat cgcggcgtcg ggcaatttac catcagtgtc
     2881 aggaatgcgc gcaacaatgc cgtcatcact ggcgaccaca gacgcgtcag cgcccagggt
     2941 tcatgcaccc gtcttccata gggagagtgc aaaataatac gccagtcgcc aatttcatca
     3001 cggcaccgtt cgaggaccag atggcgactc cccggaacga tgcccgtcgc gttgcgttgc
     3061 tcgtcaatca acccctgaat attggcgttc gtattttctt ctgccagcca cgggggaata
     3121 gtcccggaaa agaacgcgcc atccgccagt aaatgaagaa aatcgccgat catctcgcct
     3181 aattcagccg gacgtccgtt accttcacca cgccagaagg ggagccgggc agaacgaccc
     3241 ggagcaggag tcacaatcac ctgatcgcgg gtgatttgct ggatccgcca tgaggtagcg
     3301 ccgagagtga taatgtcgtt cacccgcgac tcatatacca tctcctcatc cagtttaccc
     3361 acccgccgcg aaccggcctt ttcttcacct tcgggtaata acacgctata catgccacga
     3421 tccggaatgg tgccgccgct ggtaaccgcc aacaattgag cgccaggtcg gacggtcaat
     3481 atcccggtct ccctgttcca gaccagtttg gggcgaaaag cagaaaaatc gccagaggga
     3541 tagcgcccgg aaagcatatc cagcgtggcg tcaaaaacac gtcttggcag atctttccac
     3601 ggtgcggcac ggcgtacgcg ggagtaccat tcgtctacct gtaatgcctc catcgccgcg
     3661 gcggcaacgg tttgctgcgc aaggacgtcg agtggattat gcggcggtgt caggttttcc
     3721 agcctgcctg cgaacataca ctctacaatg actgcggaat cgactaagtc acgccgggta
     3781 cgagggaaaa acagcccttt agatacaccg ccaacctgat gtccggcgcg accaatgcgt
     3841 tgtaacccac tggcaacaga aagcggcgtt gccacctgaa tcaccagatc cactgcgccc
     3901 atatcaatcc ccagttcgag actggaggtt gcgaccacgc agcgtaactc acccgatttc
     3961 agcgcctgct cggtgattgc tcgttgttct ttagagacgg agccgtggtg tgagcgggca
     4021 ataaaaacgt cgctactttg tacacggtta gaggttgcgc cggaggtcga ctcgaaatgg
     4081 gccgcatcaa cggcgataga cggggaacgc tgtaagcgtg cggcgtaaag ctcatttaat
     4141 cgtgccgtca gtttttccgc cagcccacgt gaattagtaa agacaatggt cgagcgatgg
     4201 cgcaacactt catcaaggat acccgtttca atatatggcc agatggagcc ttcccggccg
     4261 gcatggctgt cttcgccggt gctgctggcg accgatgaga catcatccat attggcgacc
     4321 ggtacgacaa ttcgtatctg cggatggcgc attgcgggcg ggttgactac cgtaaccggg
     4381 cgatcgccac caagaaaggc tgccacatcg ctggctgagc gcacagtggc agaaaggcca
     4441 attcgctgtg ctgaggtgtg gagcagcgca tcgagccgct ccagacttaa cgccagatgc
     4501 gcaccacgtt tactgcccgc taccgcgtgg acttcatcaa taattaccgt ttcgccgcgc
     4561 cgccgccgtt catcggcaat acccttcaac gggagctgca agttgcgctg aacgtcggtg
     4621 cccagagctt ttatcggtga aatatagagg atgcgtgagg ttttacgctt atgcgcctcg
     4681 cgggtatctt cgccgccttc gcggaagagc cgatcgaggg cgtagagaaa tgctgccagc
     4741 gttttcccgg agccggtcgg tgcaatcacc agcgcatgtt cgcttcgcgc cgccacatgc
     4801 caggtttgcg actggacagc ggtcggctgt ttaaaggcgc gaagaaacca gtcgcgggtc
     4861 gccggtgaaa acacgtccgg caggagcgat gaagggtctg gattatctgc cattaacgct
     4921 attccccgtg aatatcatgc aggatgcgtg gaatagcctc actatagatg cctgaatcat
     4981 tgcatgtcat caggcatcga ctcgattaca cctcttcggc ggcaggtagc ggccagcctc
     5041 ccagacgttt ccagcggttg acgatttcac aaaacagcac agcagtgcgt tcggtgtcgt
     5101 acagcgcgga gtgcgcctgg gtgctgtcga agtccatgcc agcggtctgg caagcctttg
     5161 acaatacggt ttgtccgagt gccagcccgg ccagtgcagc agtgtcaaaa gtggcgaaag
     5221 ggtggaacgg gttacgtttc agtgaggcgc gttctgcggc ggctatcata aagctgtgat
     5281 caaaattggc attgtgcgcc accataatgg cgcggttaca gccgctcgct ttaatacctt
     5341 tacgtacaac tttaaaaatt tcgtgcagcg cctcgtattc gctgaccgcg ctacgatcgg
     5401 gatcgttcgg gtcaatgccg ttgaaggcga gggcttctgg ttgcaaattt gcgccgacaa
     5461 atggttcgac gtggaaatgt aatgtggtgt ccggcatcag ccagccttgt tcatccattt
     5521 tcagggtgat ggcggcaatc tcaagcagcg catcggtttt ggcgttaaat ccggctgttt
     5581 caacatcgat cacaacagga taaaaaccac gaaaacggtc gcacagaccg gtaagttgag
     5641 cgttatcgga catctgattc tcttaatacg gataaattgc agcgcgcatt atgacaaata
     5701 ttacttgtcg atgcagtaaa gatgcgggcg cgatgagttc acgcccggca ggagattagt
     5761 tgcccagacc gcgaccggcg tctttctctt cgattaactc aattttgtaa ccgtccggat
     5821 cttccataaa cgcgataacc gtagtaccgc cttttaccgg acccgcttca cgggtcacgt
     5881 tacccccgtt ttgacggatt ttttcgcacg cttcagcggc gttatctacg ctaagcgcga
     5941 tgtgaccata agcagtgccg agttcgtatt tatccacgcc ccagttgtag gtcagttcaa
     6001 tcaccgcttc ttcggtttcc gggccgtagc caacaaacgc cagtgagtat ttgtattccg
     6061 ggttttcgct ggtacgcagc agtttcatgc ccagcacttt ggtataaaaa tcgatggagc
     6121 gttgcaaatc gccaacgcgc agcatggtat gaagaagacg catcttttta tcctcaaaat
     6181 ggctaacaga ttcaaaatgt tgttttagta tagcggcggc tttacgccgc tcgcaatgtt
     6241 ggattacaac gtcgggtaat cggtatagcc ttccgcgccg ccaccgtaga aactttcggc
     6301 acgctgtggg ttaagctcag ctttgcgctg caagcgggcg accagatccg ggttcgcaat
     6361 ccagtcacga ccaaatgcca ccgcatcaat taaccctttg ccgatcagcg tttcagcttt
     6421 ttctactgtg tatgcacctg cgccgataat cggaccgtgg aaacgggcgc gtactttttc
     6481 gcggaacgca tcagtatacg gttcaccccc cgcccaatct ggttctgaca tatgcagata
     6541 agcaatgccg cgtttaccca gttgttcaat cagatacagt gcatcggctt cttcattcgg
     6601 gccgttatcc gtgttctgga aagtaccgat tggcgaaatg cgaatgccaa tgcgatcggc
     6661 accccattct tcaatcccgg catcgaccac ttccagtacc agacgtgcgc gattttccac
     6721 gctgccgccg tactgatcgg tacgatggtt tgaagaagga gaaaggaact gatgcagcaa
     6781 ataaccgtga gcagagagga gctctaccag atcaaaaccg gcttcacgcg cgttagcaat
     6841 ggcctgacgg aaatcattga cgatacctgg aatctcttct agttcaagcg cacgcggcat
     6901 ggatgtttca acacggatcg cctgaccatt ttcatcgcgc agagaagtac gtgttcccgc
     6961 gctaagtgct gaaggcgcta ccggtgcctg accgccaggt tgcaggctgg cgtgagaaat
     7021 gcgtccggtg tgccacagct acacggccat atgaccattt tcagcatgaa cgccagcggt
     7081 gatttttttc catgcggcaa tttgctccgg actatggatg ccaggcgcac ctgcatatcc
     7141 ttttacctgg gcagaaattt gcgtggcttc actaataatc aaaccggcac tggcacgttg
     7201 gcgatagtat tccgccatca acggggtagg aatgtcaccc ggttcaatac tgcgcagacg
     7261 cgtcagcggt gccataaaaa tacggtttgc cgccgtgatc gcgcccactt tcagtgggga
     7321 atacagtttt tcagatgaca taaaggctcc cggtcgtctg gtgataaaaa atgcctgcta
     7381 aacggcaggc gtcgcaataa tgttttttac atgggccagc gcgttttcca gtggctcgaa
     7441 actgcgcgaa attttggcct gcagattcgc acccagccat agtgcgtaaa gcacttgtgc
     7501 ctgttgtagc ggttcgccac aaaaggttaa acaatggttc tcacggccat tttccagcgc
     7561 ctgagagagc agggcgatca cgccgcgagc acctttatcc atcgcgctgc gcatatcttc
     7621 tgacagatcg cacacttcgg cagagagttt tactgtcagg caaccactga tggttccatg
     7681 ttggcaaaac tggttcagtg tttgctggta ataagccagt atgcggtcgc ggtagttacc
     7741 ttcgccggat tgcagcaact cagtcagtcg ctggtgatat gcggtgtaat gacgctcaag
     7801 catggcaacg ccaaacgctt ctttagagcg aaagtagtga tagaaggacc ctttcggcac
     7861 ttcagcggtt tttagtaatt cgcttagccc catcccggtg aatccacgtt gcaggcaaag
     7921 ttgctcgccc gtcgccagga gatgttcgcg agtatcatgt tcggtgtgtt tgttcatgcg
     7981 ttggagtgta gtagaccagt cggtctagtg caagcaaagt tgctactttg gacattaaca
     8041 gcttcaataa tggtaataat cttatctgag gagggcgaag tggcggagca attagagttc
     8101 tttcccgtcc agagcccgtg ccggggaatt tgccagtctg acgaacgcgg gttttgtcgc
     8161 ggctgttttc gtagtcggga tgagcgtttt aactggaata aaatgagcga tggcgaaaaa
     8221 caagaggtgc tgcgcctttg tcggcagcga ttaatgcgca aattacgcgc aaataagcca
     8281 gcgtcatcag acgaaccgga gcaaccatca ctcttttgaa cctaatcttg cgtatactcc
     8341 tctcatatct gataagagga agcgattatg gttcagcgta ttactattgc gccgcaaggc
     8401 ccggagtttt cccgttttgt gatgggctac tggcgattga tggactggaa tatgtccgcc
     8461 cgccagctgg tcagttttat tgaagagcat ctggatctcg gcgtgaccac cgtggaccat
     8521 gctgatattt atggtggcta tcagtgcgaa gcggcgtttg gcgaggcact gaaactggca
     8581 cctcacctgc gtgaacggat ggaaatcgtc agtaaatgcg gtatcgcgac gaccgcgcgt
     8641 gaagaaaacg tcattggtca ttacatcact gaccgcgatc acatcattaa gagcgccgaa
     8701 cagtcgctaa ttaatctcgc gaccgatcat ctggatctgc tgttaatcca ccgaccagac
     8761 ccgttaatgg atgccgatga agtggcggac gcgttcaaac atctgcatca gagcggcaaa
     8821 gtgcgtcatt ttggcgtatc gaactttacg cctgcgcaat ttgccctgtt gcaatcacgt
     8881 ctgccgttta cccttgccac taatcaggtg gaaatatccc cggtgcatca gccgttactg
     8941 ctggatggca cgctcgacca actacaacaa ctgcgtgttc gtccgatggc gtggtcctgc
     9001 cttggtggtg gtcgtctgtt taatgatgat tatttccagc cgctgcgtga tgaactggct
     9061 gtggtggcag aggagttaaa cgcgggctcg attgaacagg tggtttacgc ctgggtatta
     9121 cgtttaccat cgcagccgct gccaattatc ggttcaggta aaattgagcg cgtacgggca
     9181 gctgtcgaag cagaaacact gaaaatgacc cgtcaacaat ggtttcgtat tcgtaaagcg
     9241 gcactggggt acgacgtacc gtaagcactt ttaggaatag ccgccgttca aaaatgtgtc
     9301 actggtttac acttattcag gaatgcacaa tgaacggagg tcctatgaaa cgttttagtc
     9361 tggctattct ggcgctggtt gttgcaaccg gcgcacaagc tgccagtgaa aaagtcgaga
     9421 tgaacctcgt cacgtcgcaa ggggtagggc agtcaattgg tagcgtcacc attactgaaa
     9481 ccgataaagg tctggagttt tcgcccgatc tgaaagcatt accccccggt gaacatggct
     9541 tccatattca tgccaaagga agctgccagc cagccaccaa agatggcaaa gccagcgccg
     9601 cggaatccgc aggcgggcat cttgatccac aaaataccgg taaacatgaa gggccagaag
     9661 gtgccgggca tttaggcgat ctgcctgcac tggtcgtcaa taatgacggc aaagctaccg
     9721 atgccgtcat cacgcctcgt ctgaaatcac tggatgaaat caaagacaaa gcgctgatgg
     9781 tccacgttgg cggcgataat atgtccgatc aacctaaacc gctgggcggt ggcggtgaac
     9841 gctatgcctg tggtgtaatt aagtaattaa gaggcctgcg taccttgcgg tggtgcttgc
     9901 tcaagttgcg aaagcgagca gtacagccgc cagacaattg ccgccagctc acgggcggca
     9961 ggttgatgat gacgggcaag gctgtcgcaa atccgctgta attcttcaag tgtggccgcc
    10021 agtgattttt gctgaacgcc acgctcactc atcactcccc gcaacagtga aatacagtta
    10081 tcccgtactc gcgataacgg atcggaacgc gattcccagt cgcgcaattg ccagacaaca
    10141 tgagaacagt tcagcagcac tacaccccag cgtaataacc aacggcgagc cagcgcatcc
    10201 tggctgttac tcaactgact gacgtgatga taagtgagcg attcaaattc gctttcactc
    10261 agtgttggat ggcggcttag ctgatcgaca aaatcccggc gcagcgcgcg aatatggcgg
    10321 cggcttttac gagcatccga tcctggacgc agaatggcga acgctagcca agccagcgca
    10381 acgccaacga tttttgccag attatcgtta agaaagtcag caaaatcata caccggggga
    10441 ttagtgacag cgataaacga acccataaaa acaatcagtt gcccccacag tgcggcaaat
    10501 ttgggcatct gcaatttaag aagctgcatt gtcgccagca gtggaaagag aaacagtaaa
    10561 aattgccaca gatcgctaat ctggaccatc aggccaaatt tgaccacaaa gctgaatagc
    10621 gaaagtaaca ccagcgtgcg catcagcagt gacaacgact taaacggtgc tgcgacggcg
    10681 gagtagagta cgcagctaat tgctgccagc gttaatgcat tggcaccggc atcctattgc
    10741 gatgcaatac tccatgcgcc aatcatcatc aacgtacaaa atgtacgcag cccgctccac
    10801 atagcttcgg cgttgtcggt atgacgagcc agagcggatg tccgtgcgag tctggcatga
    10861 tcatctacac cgctttgcag acgatgtaat tcctgactac tttgcagata aaggcggcaa
    10921 aaataacgta gtcgctgcca gaaggcgacg tgccggtagt cggcgacgtt ggtcgggcgt
    10981 agcggggcga taatacgtgc gacggtgtaa gcatctgttt gcgaactggc gagcgccgtc
    11041 agcaattgtt cgagaatttc tcgcgtggca cctggcggtg agggccagtt gagcaacata
    11101 cgtcgcaggc tggagatgac actggtcata cgtaattgct ggtggagtag cgcattaagg
    11161 cgcgcgtttt gctggcgaaa acgatagtgg ctccagaaag cctggatacg cagcaaattc
    11221 atggtcagta tctgcccaat ccccccctca tgtgctgcac gaatggcatc gtttgtttca
    11281 ggctgccaga gtaaactggc atgttccagt aatcgggcgt gcatgttttt caatgcggtt
    11341 aaaagggctg tagcatcgga actgctaggc aggatcatca tcatcatgcc gccgcacaaa
    11401 ataccgacta tcacctcgca aacgcgcgcc tgagcgatat cctacagctg gctggcctca
    11461 gtaatattaa ccatcggaaa ggcgatgatg gcagccgtgt agccgccagt tgaaatgcat
    11521 acgcgacgtt attcgtgaag tgcgcacagg cccaggtaca aaagccaagc cacgccgaca
    11581 tgctcaatag aaaaaaccac ggctcattga gcgtatgccc ggcaagaagc agtgccgcaa
    11641 tggctcccag taaactgcca gcgatgcgtc cgaggctttt gctgatgaca ccgccaacgg
    11701 tgggaaagct aaccactgca gccgaggtca tcgcccaata gggttcatcc agattcaaat
    11761 aataggcaac cgtcagcgcc agacacatgg caatggtatt gcgtaacgca taacgccatt
    11821 gcgccagcgt ggccttgaac cagggcaaat tgcgcaagga ccatgacgat gcgttcatta
    11881 ttgacctacg gcaatgctac aggtggtgcc cgacaccagc gtgacatcgc ctggaacttt
    11941 atccagcgca aagcggacgg gaacgcgttg ggcgagacga acccagggga cgttaggttt
    12001 cacatccgga ataaggcttg agtcactttc aacgctttga tcataaatcg cccgaccgat
    12061 actggaaacg tgaccccgta acgttttgtt atcactatag agggtaattt gtgctggtgc
    12121 accttcgcgg atatggcgca acttggtttc ttcaaaataa ccaatgacgt aaaacgagtg
    12181 gctgtcgaca agggcaaaca gcggtttccc ggtgtcagcg taatcgccga tgcgggtagt
    12241 gaggttagtc acccaaccac tcaccggggc gcgaatttcc gtttgcgcca gttgccattg
    12301 tgtctgtttc agcgtggcct gtgcggcatt aacactggcc tgcatcgctt taacattgag
    12361 gttagcggta tcgagttctt cggcagaaat aaaattttgt gagagatgac gacgacgatt
    12421 cgcttcgtta ttggcttttg cgaggtcaga ttgcgctttc gccagctgag cctgtgcgtt
    12481 cagctcggcg atctgaaagg gcgttttatc gatcgtgagc agaagatccc ccgcgttaac
    12541 cagttggttg tcttttatat tcagctcaac aatacgtccc gacacctgtg gggtgataga
    12601 aaccttttct gcgcgtattt tcccgtcgcg tgtccacgga gattgcatgt aataattcca
    12661 cagccaccat ccggcaagaa ccgcaactat cgctacaatg attattgaga aatacttaat
    12721 tgttttaatt gacatagtta ccacgcaatc agtattgcca gagcaaggca aacgcaaatc
    12781 gcaaacagcg ataaatccat ttacaaggga tgccagatgt caccggcgta gatccagcca
    12841 cgaagcaggc ggtgtacgac aagccagatg acgaatccaa acgcgaaggc tttgaaaaac
    12901 ggaggaaaat agacggacgc accgaacacc agatcttgta agggcaatcc tgttgcattg
    12961 agcataaact tcacaaagta gattccttta cgaccgaata tattgcgtta gattaataaa
    13021 tattctttaa gtgcgaaaaa tttacgcgca atttctgaaa aatatgtctg acggtaacca
    13081 aatgcagcaa tacatttgtt ttagcaataa aattgctgca cactattcta aaagccgcat
    13141 aatatcttag caagctaatt ataaggagat gaaattggaa tcgccactag gttctgatct
    13201 ggcacggttg gtgcgcatat ggcgtgctct gatagaccat cgcctgaaac cgctggagtt
    13261 aacacaaacc cattgggtta cgttacacaa tatccatcag ttacctccag accagtcgca
    13321 aattcaactg gcaaaagcga ttggcatcga gcagccatca ctggtccgta ctctggacca
    13381 actggaagaa aaagggttaa tttcgcgtca aacttgtgcc agcgatcgtc gggctaaacg
    13441 tattaaactg acggaaaagg cagagccact gatcagcgaa atggaagctg ttattaacaa
    13501 aacccgcgcg gaaatattac atggcatctc cgcagaggaa ctggagcaac tgattacgct
    13561 catcgcaaaa cttgagcata atatcattga gttacaggcc aaagggtgaa atgaaggggg
    13621 cgtgtggtca gtaacctgac cacacgcaaa cttatttagc gcggagaaac ggtcacctga
    13681 ctgccattgc tggccagtac gacacgttgg cccggagaga aacgagtgtt gccttgtttc
    13741 tgtaccacca tgatggtatt accatcgtct ttacgaattt ccagctcgac accctgcgtt
    13801 ttgttcattg cactctgtac gccctgacca gctacgccac ctgcaacagc gcctgctgca
    13861 gtagccagag aacgcccggt tccgccacca acagtattcc ccaggaaacc accaagaaca
    13921 gcaccgccaa ttgcaccgat aacgttggaa tcatcaccgc cctgaatctg taccggacgt
    13981 acgttaacga tggtgccata gctgacattc tgtacttgtt tcgcttcaga agcggtataa
    14041 acatcccctg acagggtgtc gttattaaca caaccgacaa gagacagacc taccattgaa
    14101 acaaccaata cgcgtttaat cattgaaaaa tctcctgttc accatgaaac gccacgcaag
    14161 catccctcat ggtcaaagta tatggcatat gtgaatccat ggttacatgt tctaccaaat
    14221 atgagcaaat cataatgaaa gtaggatcaa ccaattataa acaaaacatt catgatgatt
    14281 attgatttga agataatgtt ttactaccaa cggtaaaaat cggtcattcc gcatggtatt
    14341 cacgaaaagt ttatgtttga gtggtgcgca aacgtcagat tgaaaaagga tgaactatga
    14401 aatcgggccg ctttattggc gttatgtcag gcaccagcct tgatggtgtt gatgttgtgt
    14461 tggcgacaat agatgaacac cgggtcgcac agctggcaag cttgagctgg ccgatcccgg
    14521 tgtctctgaa acaggctgta ctggatattt gccagggcca gcagcttaca ctttcgcagt
    14581 ttggacagct tgatactcaa ctcgggcgac tttttgctga tgcggtcaat gccttgctta
    14641 aggaacaaaa cctgcaggcg agagatatag ttgcgatcgg ctgtcacggt caaaccgtct
    14701 ggcatgaacc gacgggcgtg gcaccacaca ctttacagat tggcgataac aatcaaattg
    14761 tggcacgcac cggaattacg gttgtcggtg attttcgccg tcgcgatatt gccttgggag
    14821 gacaaggcgc accgctggta cctgcgttcc atcatgcctt gctggctcac ccaaccgagc
    14881 gacgaatggt gctcaatatt ggcggcatcg ccaatctgtc actgctcatt cctgggcagc
    14941 cggttggcgg ctacgatacc ggtcctggta acatgctgat ggatgcctgg atctggcgtc
    15001 aggccggtaa accttacgat aaagatgccg agtgggcacg ggcgggtaaa gttattcgcc
    15061 cactgctgca aaatatgctc agcgacccat atttctcgca acctgcaccg aaaagcaccg
    15121 gacgcgaata ctttaactac ggctggctgg agcgccattt acgccatttt ccgggtgttg
    15181 atccccgtga tgttcaggcc acactggcag aactcaccgc cgtgaccatt tctgaacaag
    15241 ttttgttgag cggtggctgc gaacgattga tggtatgtgg tggaggtagt cgtaatccgc
    15301 tactcatggc gcgtctggcg gcattactgc caggcacaga agtcaccacc accgatgccg
    15361 ttggcattag tggcgatgac atggaagcat tggctttcgc ctggcttgcc tggcggacgc
    15421 tggcgggatt accaggaaat ctgccttccg tcactggcgc aagccaggag acggtactgg
    15481 gggctatttt ccctgctaac ccgtgatatc agagttaact gaatttttct taccgcgtct
    15541 gccgttacac ttggaacgtt agggagggcg tattgccctc cagaccagga aagtcttcgg
    15601 gaaatgtcta tgaaaaaact gttactgatc tgtttgccgg tgcttctcac cggctgtagc
    15661 gcgttcaatc aactggttga gcacatgcaa accgctacgc tggaatacca gtgtgatgaa
    15721 aaaccgttga cggtcaaact gaataatccg cgccaggaag tcagttttgt ttacgataat
    15781 caactactgc atctcaaaca gggcatttca gcgtctggtg cacgttacac tgacggaatc
    15841 tatgttttct ggtcgaaagg cgatgaagcg actgtctata aacgcgaccg catcgtattg
    15901 aataactgtc agttacaaaa tccacagcgt tgagattttt ccaggggcgg cgcacaatag
    15961 cgtcacccac tgacaatccg taaagaaaac catgtctgat aacgacgaat tgcagcaaat
    16021 cgcgcatctg cgccgtgaat acaccaaagg cgggttacgc cgtcgcgatc ttcccgccga
    16081 tccattaacc ctttttgaac gttggctctc tcaggcgtgt gaagccaaac tggcagaccc
    16141 taccgcgatg gtggtcgcta ccgtggatga acatgctcag ccttatcaac gcatcgtttt
    16201 actcaaacat tacgacgaaa aaggcatggt gttttacacc aacctcggta gccgtaaagc
    16261 acatcaaatt gaaaataatc cgcgcgttag cctgctgttc ccgtggcata cccttgaacg
    16321 ccaggtgatg gtgatcggta aagcagaacg actttcgact ctcgaagtga tgaaatattt
    16381 tcatagccgc ccgcgtgata gccagattgg tgcatgggtt tcgaagcagt ccagtcgcat
    16441 ttctgcccgc ggtatccttg aaagtaaatt cctggagctg aagcagaagt ttcaacaggg
    16501 cgaagtgcca ttgccgagct tttggggcgg ttttcgcgtc agccttgaac agattgagtt
    16561 ctggcagggc ggtgagcatc gcctgcatga ccgctttttg taccagcgag aaaatgatgc
    16621 gtggaagatt gatcgtcttg caccctgaaa agatgcaaaa atcttgcttt aatcgctggt
    16681 actcctgatt ctggcacttt attctatgtc tctttcgcat ctggcgaaaa gtcgtgtacc
    16741 ggcaaaggtg cagtcgttat atacatggag attttgatgg caagcagtaa cttgattaaa
    16801 caattgcaag agcgggggct ggtagcccag gtgacggacg aggaagcgtt agcagagcga
    16861 ctggcgcaag gcccgatcgc gctctattgc ggcttcgatc ctaccgctga cagcttgcat
    16921 ttggggcatc ttgttccatt gttatgcctg aaacgcttcc aacaggcggg ccacaagccg
    16981 gttgcgctgg taggcggcgc gacgggtctg attggcgacc cgagcttcaa agctgccgag
    17041 cgtaagctga acaccgaaga aactgttcag gagtgggtgg acaaaatccg taagcaggtt
    17101 gccccgttcc tcgatttcga ctgtggagaa aactctgcta tcgcggcgaa caactatgac
    17161 tggttcggca atatgaatgt gctgaccttc ctgcgcgata ttggcaaaca cttctccgtt
    17221 aaccagatga tcaacaaaga agcggttaag cagcgtctca accgtgaaga tcaggggatt
    17281 tcgttcactg agttttccta caacctgttg cagggttatg acttcgcctg tctgaacaaa
    17341 cagtacggtg tggtgctgca aattggtggt tctgaccagt ggggtaacat cacttctggt
    17401 atcgacctga cccgtcgtct gcatcagaat caggtgtttg gcctgaccgt tccgctgatc
    17461 actaaagcag atggcaccaa atttggtaaa actgaaggcg gcgcagtctg gttggatccg
    17521 aagaaaacca gcccgtacaa attctaccag ttctggatca acactgcgga tgccgacgtt
    17581 taccgcttcc tgaagttctt cacctttatg agcattgaag agatcaacgc cctggaagaa
    17641 gaagataaaa acagcggtaa agcaccgcgc gcccagtatg tactggcgga gcaggtgact
    17701 cgtctggttc acggtgaaga aggtttacag gcggcaaaac gtattaccga atgcctgttc
    17761 agcggttctt tgagtgcgct gagtgaagcg gacttcgaac agctggcgca ggacggcgta
    17821 ccgatggttg agatggaaaa gggcgcagac ctgatgcagg cgctggtcga ttctgaactg
    17881 caaccttccc gtggtcaggc gcgtaaaact atcgcctcca atgccatcac cattaacggt
    17941 gaaaaacagt ccgatcctga atacttcttt aaagaagaag atcgtctgtt tggtcgtttt
    18001 accttactgc gtcgcggcaa aaagaattac tgtctgattt gctggaaata atgcattaag
    18061 tggaaagggg gagtgagaaa tcactccccc tggtttttat acagggaaca tgatgaaaaa
    18121 tattctcgct atccagtctc acgttgttta tggtcatgcg ggtaacagtg cggcagagtt
    18181 tcctatgcgc cgcctgggcg cgaacgtctg gccgctgaac accgttcaat tttctaatca
    18241 cacccagtac ggcaaatgga ctggctgcgt gatgccgccc agccatttaa ccgaaattgt
    18301 gcaaggcatt gccgccattg ataaattaca cacctgtgat gccgtgttaa gtggctatct
    18361 gggatcggcg gagcagggtg aacatatcct cggtatcgtc cgtcaggtga aagccgcgaa
    18421 tccgcaggcg aaatattttt gcgatccggt aatgggtcat ccggaaaaag gctgtatcgt
    18481 tgcaccgggt gtcgcagagt ttcatgtgcg gcacggtttg cctgccagcg atatcattgc
    18541 gccgaatctg gttgagctgg aaatactctg tgagcatgcg gtaaataacg tcgaagaagc
    18601 ggttctggca gcgcgcgaac tcattgcgca agggccacaa attgtgttgg ttaaacacct
    18661 ggcgcgagct ggctacagcc gtgaccgttt tgaaatgctg ctggtcaccg ccgatgaagc
    18721 ctggcatatc agccgtccgc tggtggattt tggtatgcgc cagccggtag gtgttggtga
    18781 tgtgacgagc ggtttactgc tggtgaaact gcttcagggg gcaacgctgc aggaggcgct
    18841 ggaacatgtg accgctgcag tctacgaaat catggtgacc accaaagcaa tgcaggaata
    18901 tgagctgcaa gtggtggctg ctcaggatcg tattgccaaa ccagaacatt acttcagcgc
    18961 aacaaagctc tgaaatatct catttcggcc cacaacgacc tgtgggccga tggctattac
    19021 tttaagcctt ccgctgacag tgcgtcttgt acttccggac gttcagccat acgttgcata
    19081 aatgctgcaa tgtgctctaa cccttccaga ttcagtttca ccgcgtatgc ccagcgcaga
    19141 accgtaaaca gataggcatc agcaattgta aatctttgcc cgcagatcca gtgctcatcc
    19201 ttcagtgtct cgttcacata ttgcagcttc ttctccagct gcgcgcgaac tgtcggtttg
    19261 tactcttccg gtgtatcagg gcgaaacaga ggtgtgaaac ctttatgcag ctcggtggcg
    19321 atgtaattca gccattcgat ggttttatag cgggaaatac tgtttaccgg tgccagcaac
    19381 tggcggtcgg ggacgctgtc ggcaagatac tgcataatcg ctacgccttc cgtcagcaaa
    19441 gtaccgtcat ccagcagcaa tgcaggcacc tgccccttag ggttaacggc aaagtaatcg
    19501 tcaccgtttt cgagacgttt tttcattaaa tccacactga cgagggtaaa atcctttccg
    19561 ctctcacgca gggtgatatg ggaagcgaga gagcaggcac ccggtttgta gaacaatttc
    19621 atcggtaact ccttttgtct gtggtttaaa gtatggtagt gcgttgctga acaaaaaaaa
    19681 agccgctaac gaaaagttag cggcttgtaa aagagtttcc ctaaatttta cgctacagca
    19741 gctttcgccg ctttgtctgc agcgtcatcc tgcgtcatac ggtgcagttt aggcgcggtc
    19801 agcagcatca gtactgcaat aatggcagta gcgacaccaa tctgcaagaa tacgcgacca
    19861 taaacttcca gtgacatcag cgggtcggta acgttatccg gcacagccat catacccgca
    19921 acataaccac caatcaggtt tgcaccggca gtggtcagga accagctacc cataatgaag
    19981 cccatcagac gctgcggaac gagttgtgca accattgcca gacccagacc agagatcatc
    20041 agttccccga tgctctgcag gccatagctt gcgaccagcc agcttacaga cacgatacca
    20101 gcgtcagacg cgaatttcgc acccagcggc agaatcagga acgcaccaga acacatcacc
    20161 atgccgattg caaacttggt tggcatcggc agggtatcgc ccatcttgtt atagatagcg
    20221 gccagaatcg gactaccgat gatgatccag aacgggttca gtgcctgata ctgctcaggt
    20281 tctacggcca gacccagaat ggagtgctca acgttacgaa tcgcaaagaa gttcagtgac
    20341 gttggcatct ggctgtacag cacgaagaag ataatggctt cgagcatcag gatgaaggca
    20401 acgatcattt tacgacgcgc agcacctttc atcgcgaagg cttctttacc gaagataacc
    20461 acgataccga aggcaacaac gcccagcgcc atacgcgcaa cttcctgatt gtgcagcagc
    20521 caggtggcga tagcgatcag tgccacaaca ccaataatgg tcagtagcag gttacggtag
    20581 ttgataggct cgaagtctgg ttttgaaccg tactgtttaa cccagcgttg gcagaaggcg
    20641 aagttaacga tagtgatcag caggcctaca acgctcaacg caaacgcaac actccagccg
    20701 tatttcgcgg ccagccacgg cgtagcaatc atagagaaga aagagccgat gttgacggac
    20761 atgtagtaca tggtgaatgc accgtccaga cgcgggtcgt ttttctcata gcatgtagaa
    20821 agcagagaag acgggttagc tttaaacagg ccgttaccga ccgcaatagc cgccataccc
    20881 atataaacga taccggcgtc gtgaccagac caggcaacca gagcataacc aatcgccagc
    20941 acaatagcgc cgagcataat tacgcgttta gtacccagta ccttgtcacc taaccagccg
    21001 ccgatagcga ccagaccata aaccagggca ctaaaggaag agaaaagggt gattgaatcc
    21061 gcttcagaca tacccagttg tttaaccagg taaacagcca taattccttg taggccgtaa
    21121 taaccaaaac gttcccataa ctcaatcgag aagatgagat agaacgcctt cggttgtttg
    21181 aaagcgttca aactgacgct ttcagttggt ttttggtttg cagtggacac ttttacctct
    21241 ttttttacat cccatattaa cgggggtgct ttctcgcgtt atgtctaaaa aacatacgcg
    21301 caattgttat agtgggaggg gaaacggcag gtaatgttca ctatcttgca ctttcagaca
    21361 agtcctttgt aataatttgt tacatataac tgaggcgggg aattcaacca ttccgggaaa
    21421 tgttatccag cgttaaattt ttcaaaaaac gtaacggcag attttttcac tgcactgaga
    21481 ggggggtgat ggcttaacgg cgatatgttc tgctatttag cgtttttact agtgagatag
    21541 tccagtttct gaaaaatagc cagtgtaatg ttttgtaggt caatatttgt tgcgtgtgta
    21601 aatttaaatg attttaacct gattcttgca atgtttttac attgcgacag catcgtgatc
    21661 ttgatcacga atgaatagaa aatttcgcta ttaaaaaaac aattaacctg catttttggt
    21721 tatccgcaaa cggattacct gatgataacg ctaagcaatg gcatcattca cgttaaaaag
    21781 atgttttaaa agaggataaa gaaaggttat caatggggta atcggtgtta ccccttttct
    21841 tcagatgtca actttctctt tgtattcaca aagatcttca ataatacaag agccacagcg
    21901 gggcttgcgg gcaatgcagg tataacgccc gtgcaggatc aaccaatggt ggcagtcgac
    21961 tttaaactct gccggaacca ctttcagtag cttttcttct acctgttcaa cgtttttccc
    22021 cggcgcaaat tgagtacgat tacaaacgcg gaaaatgtgc gtgtcgacag caatagtcgg
    22081 ccagccgaat gcagtgttta atacgacgtt ggctgtttta cgacctacgc cgggcagggc
    22141 ttcaagcgca gcacgatctt ccggaacctc gccattatgc tgctccagta agatacggca
    22201 ggttttgatg atattttctg ctttgctgtt ataaagccca atcgttttga tataggtttt
    22261 aaccccttca acgcccagtt caagcatcgc tgcaggcgta ttcgccaccg ggtagagttt
    22321 cgccgtcgcc ttattaacac tgacatcggt cgcctgagcg gaaagcagta cggcaatcag
    22381 caattcaaaa ggcgaactga aattaagctc ggtggtggga tgaggattgt tctcacgcag
    22441 gcgagtgagg atctccaggc gttttgcttt gttcatcaga cattccctgt ttcaccgttt
    22501 ggcaatgcac gttcggcagc tgcttcagta cggcgctttt tcattctttc atcaatcagg
    22561 tattttcctg ccagcatcag tcccaggcca ataaatgcac ctggtggcag catcgccagc
    22621 aggaaagggg agtcggtgtg gaaaatctcc acgcgtaata cttttgccca gctacctaac
    22681 agcgcatctg caccgtcaaa caatgtgcca ttgccgataa tttcgcgtag tgaacccagc
    22741 acgaacatgg cgcaggttgc gcccatacca attgaaaagc cgtccagtgc cgaaagcgcc
    22801 ggaccttttt tggcggcgaa ggcttcagcg cggcccacaa cgatacagtt agtgacaatc
    22861 agcggaataa aaatccctaa tgattgatac aggccaaagg cgtaggcgtt gatcagcatc
    22921 tgtacagcgc tgaccaccga ggcgatgatc atcacgtaaa tgggaatgcg gatctcggct
    22981 ggcgtccagt gacgcagcgt cgaaatggtc aggttggtca gcgtcagtac cagcgtagtc
    23041 gcaagtccta aacccagagc attagtggca gtggacgtga ccgccaacag aggacaaagg
    23101 ccgagcaact ggaccagcgc agagttgttt ttccacaacc cctgaacaat aacgtcttta
    23161 atttcgctca cgctttattc tccacaggca ggaagttgag aaagttgtgc cggtaacgtc
    23221 tgagcgtaca atccggcgcg ttttaccgca ttaaccaccg cgcggggagt aatcgtcgcg
    23281 ccggtgaact ggtcgaaatc accaccatct ttcttcaccg cccagtgcgc atcatccgca
    23341 ccactgattt ttttacccgc aaaatgggtg atccagtcag aaaggcgcag ttcgatttta
    23401 tcgccaagcc ctggcgtttc gtggtgctct gtcacgcgcg tgccaagtac cgtgccgtta
    23461 aaatcggctc ccaccagcag ctgaatcgca ccggaatagc catctggcgc ggttgcttcc
    23521 agaacggcgg ctaccggttt gtcatcctgt ttggcgatgt aaacccgatg ctcaccttta
    23581 cctaactctg gcgcagttac cagatagcaa ctctgtgcca gcgcattgtt atagcgttcg
    23641 gctggcagca cctgatcaaa taatgccttt tgttgcagac ttgcctgttc agcaatcgtc
    23701 gttttggtca tctggttgat ggccgcagtt aaccctgttg aacccgctgc aaatagcgct
    23761 aacgtaatgc cgtgttttcg gatagttttc agcatggttt accctttgcg atggccgtag
    23821 acgcgcggac gcgtgtagta atcgatcaga ggaaccgtga tgttcgccag caggacggca
    23881 aaagccacgc cgtcaggata gccgccgaaa ctgcggatca accagactaa taagcccgca
    23941 agcgcgccga aaataagacg accacgattg gtcgtagaag cggtaaccgg gtcagtcaaa
    24001 ataaagaatg cgccgagcat ggtcgcacca gacagcagat gaatttgcgg tgctgccagt
    24061 gtttctggtg agaacaacca gcccaacgtt gcgcataacg ccagcgttac taagaagctg
    24121 aggggaatat gccagcgaat cgctttctgc catagcaacc acaggccgcc agccagccag
    24181 gcgagattta cccattgcca accagcgccc gccagaatac cgctgtagat cggatattgc
    24241 ataatctgtt caaccgaatg accggcacgg acagaggttt taaatgtatc cagcggtgtc
    24301 gcctgactaa tgccatcaat acctaagtgt agtgtgttca tatcagcacc actggcggta
    24361 tgcccgctga aaataacctg tatggcgtcg ataaaaccag ggatgttgac cgcaatttca
    24421 tgtggcggta accagctggt catctgcacc gggaaggaga tcagtaagac cacataacca
    24481 atcattgccg gattaaacgg gttttgcccc agaccgccat acaactgttt agcgatgatc
    24541 accgcaaaca ccgtacccag cacgaccatc caccatggcg cgaggggggg aatacttacc
    24601 gccagcaata agcctgtcag caatgctgag ttatctttca acgttgcggc taccgactgc
    24661 ttgcgtagtt tgagtacgag agcttcggct aacagagtac taaccgatgc caacaggatc
    24721 tgaacgagag taccccaacc aaaaaaccac agttgcgctg cgattcctgg cacggctgcg
    24781 agcaacacca acagcataat gcgcgatgtc tggcgctggt tatgggtata aggggagcta
    24841 gctattctga ataccattta gtcctcgttt acaacctttt gctgggcggc ttttttggcc
    24901 tgagcgcggg caatagccgc ggcaactgcc gctttgcgcg gatcaacctg ttcttctggt
    24961 tctgcgttag tctgttgctg ttccagcttg cgcgctttgg cacgggcaat agcggcttcg
    25021 acggcggctt tgcgcggatc aacctgttct tcaggtaccg cattagcctg ttgctgttcc
    25081 agcttgcgtg ctttggcgcg tgcaatagcc gcttcgacgg cagctttgcg cggatcaacc
    25141 tgttcttctg gctccgcatt agcctgttgc tgttccagtt tgcgcgcttt ggctcgggca
    25201 atagcggctt cgatggcggc tttgcgcgga tcaacctgtt cttctggttc cgcattagcc
    25261 tgttgctgtt ccagcttgcg cgctttggca cgggcaatag cggcttcgac ggcggctttg
    25321 cgcggatcga cctgttcttc tggttccgca ttagcctgtt gctgttccag cttgcgcgct
    25381 ttggcgcggg cgatagctgc ttcaacggca gttttacgtg gatcagcaac ggttgctgcg
    25441 tcgttagttt gctgcagttc tgcctgtttc gctctggctt gcgctttacg ggcttcccgt
    25501 gctgcaataa ttgcactgtt atccgggcgt tcgcccgctt taatcacaat aggctgtgta
    25561 gcctgggcct gtttctcttt cacccgcgcc agagcggcag caatcgcatc tttatctttg
    25621 gctgcaggtt gaacggctgc gctcttatgt cgttcaaggc gagccgcttt ttcgcgctcc
    25681 agacgagcct ggcgcgcttc gaaacgcgct ttggcttctg cggcgcgctt ttcttcctga
    25741 cgaatagccg caatttcagc tttttcctga cggaaatatt gcaccagggg aatattgctc
    25801 gggcaaaccc acgcgcaagc cccacattca atgcaatcag caatgttatg cgtggtagct
    25861 ttatcgtgtt gctgaccttt gctgaaccag tacaactgtt gcggcaaaag atccgcaggg
    25921 caggcgtcag cacaggcgct acaccggatg cagctttgtt cttcctgtgg ttcgccaagt
    25981 tcattggcag agggagccaa cagacagttg gtaatcttta cgaccgggac atccagccat
    26041 ggcaaggtaa agcccattag cgggccaccc ataatcacca tttgatcggc agaggggcag
    26101 aatcgggcat cattcaataa atgacgcact ggcgtcccca gccgtgccca gacgttgccc
    26161 gggcgagcga ttgcttcgcc agtcagggtt acaacacgct cggtaatcgg ctcgccatca
    26221 ataacggcac gtttcactgc ataagcagtg ccgacgtttt gcattaatac gccgatatcg
    26281 gatgaacgcc cgccatgtgg aacctgcttc ccggtcagaa tgtaggttaa ttgtttagca
    26341 ccgccagaag gatatttggt tggaatcacc cgcagagaaa tatcgttaga gtccgccagc
    26401 accgcgcgca gcatggaaat cgcctgcggt ttgttatctt caatgccgat aagaatttcg
    26461 cgtggctgca gaatatgcgc aagaatgcga ataccctcta cgacctgagc cgcgcaatcc
    26521 tgcatcaaac ggtcatcggc ggtaatgtac ggctcgcact cagccgcgtt gataatcaac
    26581 gtttcaatct tatctccgcc accctgcaat ttaacgcctg tcgggaatcc tgcaccgccc
    26641 agcccggcaa caccaaactg atgtatgcgc tcgattaact cttcgcgact acgagtgcga
    26701 taatcggccc agccgtcgcg cgggatccag cagtcttcac catcggcatc aataatcacg
    26761 cttaattcag ctaaagctga aggatgagcc gtagagtggg gcgcaatagc cgtaacggta
    26821 cccgaggtgg gcgcgtgaac aggcagcatt ttgccgcgac cacgggtaag cggctggccg
    26881 cgcaatactt tatcgccgac gctaacgcac aactcacctt cagcgccaat atgctgtttc
    26941 agtggaataa caaaacgctg cgccagtggt acctggcgca ggggtgtacc gttggactgg
    27001 gttttcatct ccggtggatg gatgccgccg ttgaaatccc agattttatt ttttctgaat
    27061 gcagagaata acttaagcat ggagttccac gggaatgata cgcacgggaa tggtgttcag
    27121 atcccatttc caggagtcag gtgtttctgc gaccggttgc aacgagatgc agtgcgtcgg
    27181 gcacggatca acacataaat tgcagcccgt acagagatca ctcattaccg tatgcatggc
    27241 acgggtagcg ccaacgatgg cgtctaccgg acaagcctga atacatttag tgcagccaat
    27301 acagttattt tcatcaataa ccgccaccat ccgcgcaggc gttagctctt gcgcttcgcc
    27361 atccagcggc tgcggctcga cattaagcaa ctcggcaatt tttagcatca cagcttcgcc
    27421 acctggggcg caacggttga ttttttcacc gttacagctg atggcttccg cgtaggggcg
    27481 acagccggga taaccgcact gaccacactg gctctgcggt aagatttcgt caattttctc
    27541 aacgaccgga tcgtcttcca ccgcaaaacg gcgggaggca taacccagaa tggcgccaaa
    27601 cgccaggccc agcaggctca cggcggcaac ggcaatccag atagcattca ttacaacttc
    27661 accaaaccac taaagcccat aaaggccaga gacataagac ctgcggtaat taacgcaatg
    27721 gcattaccgc gaaaaggtgc cgggacatca gccacagcaa ggcgttcgcg gatggcggtg
    27781 aagagcacca tcaccagcga gaaaccgaca gcggcggaaa aaccgtacag cgccgactgc
    27841 aagaaattgt gcccgagatt gatattcagc aacgccacgc cgagcactgc acagttggtg
    27901 gtgataagcg gcaaaaaaat ccccagcaga cggtaaagca ccgggctggt tttgcgcacc
    27961 accatctcgg tgaactgcac gaccacagca atcaccagaa taaatgccag ggtgcgcagg
    28021 taaatcagat taagtgggat caaaatccac gtatcgataa gccaggcgca aatagacgcc
    28081 agcgtcatca caaacgttgt tgccagcccc atgcccatcg cggtttccag ttttttggaa
    28141 acccccataa acggacagag accgagaaac ttgaccagca caaagttatt gaccagtaca
    28201 gttccgacaa acagtaacag gtagtcagtc atttttcggc ctgaaataaa aaagccgcct
    28261 attatccgtt aatccagagc aggcgacaac aggttaactg taaggttatt acggattcac
    28321 aaacgtcgct tttacccgcg acgagcgttt gaaataggag acgatcaatg cggttgccaa
    28381 caaagtaaac agcaactggc gaacggctat gccatcttcc acgggtgaaa aagcgaatgc
    28441 ttttactgcc agcaatacgg aaataagcag ccagataata tagtgtttag gaacacaacg
    28501 ccgacgttta aagaaagcga tggtcaacca cagtgtgtaa taccacatgg cgatagcggt
    28561 aataaaagaa acgccccata agatttgtgt cgtcagtgct tgtccgccga gcgtttgaaa
    28621 tgtttgcgga gaagataacg cagcggtata cagcaacaac gccagcgtcg tactcaacag
    28681 agcgactaaa agccaggcca acgggccaag caaccagcct ccaatacgtt gtggcgttgt
    28741 ggtggtcata ggttctccag aatagcgacg caaacaggca aatctaattt taggggcgtt
    28801 attataaggc ttttataacc gatcgctact ttctttattg gacatagtgc cagacggact
    28861 tcggtacctg gccgaggtca aacaaacgcc cgccagaaac cagctctgcg cgacggtgat
    28921 cggcagcacg atacatattg atcagttcct gatcgtccgt cagggtgtaa ttaagatggt
    28981 cgtagagttt ttccagactt tcgagtgaac tgattttgcg aaattttaat aagtagtcct
    29041 gaacagtcat aaaaggtcca taaaataatt aataccaaag gggtaggtat tattagtgat
    29101 gataaaattt caccaaaagt gaacgcttgt gttattacaa taataagaat gcgctgatgg
    29161 caaatattca gggatgaaaa actgctctga aaagagtaac gcgctttact atccctgaca
    29221 atcaccaaca acatcgaaca agataataaa ttcctggttt aatatccgac aagtgaaaac
    29281 atgcaaccgg acgggcagca tggcgctcta caagcgcaga gcttacttgt gttgtaccga
    29341 agcactctgt tcaggtggct gatagttgtc aatgtgactc gccacgccaa gaagaatgac
    29401 tgaaacgaca agaacgatcc aacctgttaa ttcaataaga cgattcatta cagcccacat
    29461 ctctcttgat tgatccatta acttcagggg gtaaatgtta cttagcaata atagctccag
    29521 caagattttt actgaggttt ttgcgatatt tagcttttgt cgttggaaaa ttcgctattt
    29581 ttttgactta atattaaact gttgagcgtg ggtaaaggat agtgtcaaat agcaatcata
    29641 cttcaatgag aggcaatgac atgagcgaca acatccgtgt tgggttgatt gggtatggtt
    29701 atgcgagcaa aaccttccat gcgcccctga ttgcgggcac gcccgggctg gaactggcgg
    29761 taatctccag cagtgatgaa acaaaagtaa aagccgactg gccaacggtt acggttgtct
    29821 ctgagccgaa gcatctgttt aacgatccca acatagacct gattgtcatt cctacaccca
    29881 acgataccca tttcccgtta gccaaagcgg cgcttgaggc gggtaaacat gtggtcgttg
    29941 ataaaccctt taccgtgaca ctgtcacaag cgcgagagct ggatgcgctg gcaaaaagcc
    30001 tggggcgtgt gctgtctgta ttccataacc gtcgttggga tagcgatttc ttgacgctaa
    30061 aaggtttgct cgcggaaggc gtgctgggtg aagttgctta ctttgagtct cattttgacc
    30121 gcttccgtcc gcaggtgcgc gatcgttggc gtgaacaggg cggcccaggc agcggtatct
    30181 ggtacgattt agcaccgcat cttcttgatc aggccattac gctatttggt ttaccggtca
    30241 gcatgacggt agatttggca cagttacggc ccggagcgca gtcgaccgat tatttccacg
    30301 ccatcttgtc ctatccacag cggcgagtca ttttacacgg taccatgctg gcagccgctg
    30361 agtcagcacg gtatatcgtg catggatccc gaggcagtta tgtgaaatat ggcctcgatc
    30421 cacaggaaga acgtctgaaa aatggcgagc gtctgccgca ggaagactgg ggctacgata
    30481 tgcgtgatgg cgtacttacc cgcgtggaag gtgaggaacg tgtcgaagaa acgctgttga
    30541 cggtacctgg gaattatccg gcttactatg cggctattcg tgatgcgtta aatggcgatg
    30601 gtgaaaatcc ggttccggca agccaggcaa tccaggtaat ggagttgatt gagctgggca
    30661 tcgaatccgc caaacatcgc gcgactttgt gccttgcatg aaaaatggat ggtgcaattt
    30721 gcaccatcca ttttgttact tcgcggcgac tttttctcgc agtgcgcgtt tttcctcagc
    30781 gctgaggaaa gccatttcca gaccattaat ctgtgcctgg cggatttgct cgcgggataa
    30841 cccagcagct ggcgcggcaa cggtatattc gtgaatgata tccactccct gtacgccggg
    30901 atcgtcagtg ttaatgccgg cacgaatgcc atgctcaagg aacgttttca gcggatgtgc
    30961 agccagctct gctacggtgc tggtctgaat attggaggtc agacaggatt caataccaat
    31021 ttgttgctcg gcgagaaaat ccatcagcgc ccgatcttca atggctttta cgccatgtcc
    31081 aatacgctcc gcacccagtt cacgaatcgc ctgccagatg ctttccggcc cggcagcttc
    31141 gcctgcatgg acggtaatat gccagcccgc atcacgcgca aggttgaagt gagaaaggaa
    31201 cagacttccc gggaaaccaa gttcatcacc ggctaaatca agggcggtaa tctggtcacg
    31261 gtgggctaaa aaggcctcca gctcttgctg acaggcggct tcgccgaagg ttcggctcat
    31321 aatgccgata agcttcgcct gcacaccaaa agtgcggcaa ccttcacgta cgccatcgat
    31381 caccgcttcg acaacacccg ctacaggcag cttatgtgcc attgccatgt agcctggtga
    31441 aaaacgcagc tcgacatagt gcaggccgtt acgggctgca tcttcaatgt tttcaaatgc
    31501 cacgcggcgg caggcatcaa gagaggcgag aactttaacg ccccagtcaa gtttggtcag
    31561 aaagctcacc agatcgggtt cgttggcaat gacctgaacg tggggaatca gtgtttccag
    31621 ggattgtgca ggaagcgaga tattatactg gcggccaagt tcaagaatgg tctggggacg
    31681 aatgttgcca tcaaggtggc gatggatatc agttaatggc agggtggtat caatcatggt
    31741 cgcactcttt ttctggatat aaaagtgcgc accattataa aaacaaagca gggtaaaaat
    31801 ccacgattgc gcaacgattt ttacgttgcg caattggggt taacgaacag cgcggatggc
    31861 gttaattaat ccagccacac ctttttccag tttcgaacgt gggcagccgg cattgagacg
    31921 gacaaaacca cgaccttctt caccgtaggt ataccccggc atgatcgcga ctttttcttg
    31981 ttcgataagt gctttttgca acgcgttgtc gtcaatattc aacggacgta aatcaagcca
    32041 tgccagataa gtggattgtg ggatctgcca gttgagttca ggaaacgcgg cgttcatttt
    32101 atctgcgata tacgtcaggt tatctttcag atagatgcgt aaggcatcca gccacggcgc
    32161 gccttgctga taggcggcga tatgggcagt taacgccagt accgaagggg aagaaagccc
    32221 atcacggcct ttcagtgccg ataaataggc atcgcggcta ctgctatttt ctataatccc
    32281 gtaagcaccg gtcagggcgg gaatattgaa acttttcgag cccgacgtta gcaacgccca
    32341 gtctccgcga gccacattac tccagggaat atgcggctgc tcgccccaaa ccatatccat
    32401 atggatttca tcggaaataa cccgcacacc atgacgctcg cacaggtcag ccatgatctc
    32461 cagctcatcg cacgtccaca ctttcccggt aggattctgt gggctacaca ggagcataat
    32521 tttacattct ggtttcgcca acacggcttc caacttgccc atatcgcaaa accaaccatc
    32581 agcctgcttc tctaaagcaa cgggcattac tgtgcgctgg ttaccttcaa tggccttgta
    32641 aaatgcgtca taggcgggtg tgtggatcac cacgccttca cctgtttcag accactgacg
    32701 aatcagttct gaaaccatat agatgacaga agggccatac accaccgtct gagaatcgat
    32761 ggcggtgtaa tgctgggtgg aaaaccagtg ggcaatagca gcgagaaact catcgttttt
    32821 ccagcggctg tagccaaata cgccgtgcat caggcgctga ttcagcgcct ctataatgca
    32881 gggggcagtg gcaaaatcca tgtctgaaat cgtgaacggt aacaggtcag cagtgccgaa
    32941 acggtcagcg acataatccc actgtgtaca ccatgtgcca tgacgatcca cgacctttga
    33001 aaaatcgaac atatcttatc cttatgcctg gacagtatgc atcagaccgg ccatttcatc
    33061 ttttactgac tgaacttgtg gcccgataac aacctgcagg ttatgttgat taagttgtac
    33121 tacgccaatt gcccgattgt ccttcagtgc ctgcacatta acaagcgaca tatctttcac
    33181 agacaaacgc agacgggtaa tgcagttatc gaggctgaca atattgtcgg caccgcctaa
    33241 tgcttcgagg attgcaggaa cgttgtaacc tgatttaccc ggcgcagcgg caacggcttt
    33301 ttcgattgag ctggcaactt cgctatcgcg ccccggggtt ttgagattga agcgggtgat
    33361 agcgaaacgg aagatggcgt agtaaacgac aaaccagatt gccgccacca ctggcaccat
    33421 gtaccacttg gttgacagac catgcaaaat accgaacacc acgaagtcga tgatattgcc
    33481 gtcggtatta ccgatggtga cgccgagcac agacatgacg gtgaagccga ggccggttaa
    33541 cagcgcgtgg atgacataca gaactggcgc tacgaacagg aacaggaatt ccagcggttc
    33601 ggtagtgccg ccaacgacgc aggcgatcag gccagaaatc agcagacctt taattttatg
    33661 gcgattttcc gggcgcgcgc agtgatacat agctaacgct gcagctggca gaccgccgag
    33721 aaacgcaggc attttacctt gcgaaaggaa acgcgtggcg ctttcagaaa aaccgtgagt
    33781 ggtcgggcaa ctcaattgcg tctggaagat ggtcagtgcg ccgctgacgg tttgaccgca
    33841 gacttcctgc gtgccgcctg cgtcggtaaa gcgaattaat gccaccagaa tgtgatgcag
    33901 accaaacggc aacagcagac gttcaccggt accaaacagc atcggtccga aatcacccgc
    33961 gctgtttatc atatggccca agccgctaat acccatggcg aaaatcggcc agactaatgg
    34021 aatcaccagg ccgacaaggc ccatcaccag cgaggagata attggtacga agcgcgtacc
    34081 gccgaagaat gccagcgcat ccggcaggcg gatattatgg aaacgctcat gcagcatcca
    34141 gacgataata ccggcgatca ccgcaccgag gatcccggta tcgatcgact ggatcccaag
    34201 aatgctctgg atgttattgg ctttcagaac cgcggcatcc gtggttggca gaatgccttt
    34261 attggtcaac cagaagttta ccgcgaggtt cattaccgca taaccgacga agccagcgaa
    34321 tgccgctacg cctttatttt cgcgtgccag gcccagcggg atggcgatac agaacatgac
    34381 aggcaggaaa ctaaaagcaa acgagccaat cttactcatc caggtaaaga tagcctgcaa
    34441 cacggggttg cccaggaccg ggatcagggt tatgacatca tggctgctaa gagaactacc
    34501 aatgccgagc ataatgccgc agaacgacaa taatgccacg ggtaacatga aggttttgcc
    34561 taactgctgg aagaactccc acagcgtgac tttcggtgct gttttcgccg tcataaaacg
    34621 actcctcgta gagaacaatc tggattcagt aaattgcgcg agaagataaa acgttttatc
    34681 aaattttagt gaggcataaa tcacattacg caacgataat agcgggtata agataaataa
    34741 aaggtaaaac gttttatctg tcacataatc agggagtagg tcatctgcat ggctaccgcc
    34801 aaaaaaataa ccattcatga tgttgcgctg gctgcgggcg tgtcggtaag taccgtttcg
    34861 ctggtgctta gtggcaaagg gcgaatctct accgccacag gagaacgcgt taacgccgcc
    34921 attgaagagc tgggatttgt gcgcaatcgc caggcgtcgg cgctgcgcgg cgggcaaagc
    34981 ggcgtcattg gtttgatcgt ccgtgattta tctgcgccgt tttacgccga attgacggcc
    35041 ggattgacgg aagctctgga agcgcaggga cggatggttt ttttgcttca cggcggtaaa
    35101 gacggcgagc agctggcaca gcggttttca ctgttactga atcagggggt cgatggtgtg
    35161 gtaattgccg gggctgcagg aagcagcgat gacctgcgac ggatggcaga agaaaaagct
    35221 atcccggtga ttttcgcttc ccgtgccagt tatcttgatg atgttgatac ggttcgcccg
    35281 gacaacatgc aggctgcaca gttgttgacg gagcatctca ttcgcaatgg gcatcagcgg
    35341 atcgcctggc tgggagggca aagttcctca ttaacccggg cagaacgggt gggaggctat
    35401 tgtgcaactc tactaaaatt tggtctgccg tttcacagcg attgggtgct ggagtgcaca
    35461 tccagccaga agcaagctgc ggaagctatc acggcgcttt tacgtcataa cccgaccatc
//
LOCUS       DAJTSL010000024        35005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTSL010000024 DAJTSL010000000
VERSION     DAJTSL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.024"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            32..1270
                     /gene="dsdA"
                     /locus_tag="OO433_001043"
                     /pseudo
     CDS             32..1270
                     /gene="dsdA"
                     /locus_tag="OO433_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OO433_001044"
     CDS             complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OO433_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6049598.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OO433_001045"
     CDS             complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OO433_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6049599.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYVYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            4495..5109
                     /gene="evgA"
                     /locus_tag="OO433_001046"
     CDS             4495..5109
                     /gene="evgA"
                     /locus_tag="OO433_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6049600.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGASAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            5114..8707
                     /gene="evgS"
                     /locus_tag="OO433_001047"
     CDS             5114..8707
                     /gene="evgS"
                     /locus_tag="OO433_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6049601.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILITFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OO433_001048"
     CDS             complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OO433_001048"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6049602.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDYDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OO433_001049"
     CDS             complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OO433_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6049603.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OO433_001050"
     CDS             complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OO433_001050"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6049604.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVIGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSSVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OO433_001051"
     CDS             complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OO433_001051"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6049605.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(14507..15139)
                     /locus_tag="OO433_001052"
     CDS             complement(14507..15139)
                     /locus_tag="OO433_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6049606.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            15435..15710
                     /locus_tag="OO433_001053"
     CDS             15435..15710
                     /locus_tag="OO433_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049607.1"
                     /translation="MKVNLILFSLFLLVYIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(15787..16029)
                     /locus_tag="OO433_001054"
     CDS             complement(15787..16029)
                     /locus_tag="OO433_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6049608.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTEIFMFLAPACGLMIRFMVGYGRR"
     gene            16382..17302
                     /gene="lpxP"
                     /locus_tag="OO433_001055"
     CDS             16382..17302
                     /gene="lpxP"
                     /locus_tag="OO433_001055"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6049609.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHCRFKTRPVGESSLYI"
     gene            17658..17729
                     /gene="ypdK"
                     /locus_tag="OO433_001056"
     CDS             17658..17729
                     /gene="ypdK"
                     /locus_tag="OO433_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6049610.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OO433_001057"
     CDS             complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OO433_001057"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6049611.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRQDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWIVEMPKASMYVWAKI
                     PEPYAAMGSLVFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            19409..21105
                     /gene="ypdA"
                     /locus_tag="OO433_001058"
                     /pseudo
     CDS             19409..21105
                     /gene="ypdA"
                     /locus_tag="OO433_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
     gene            21120..21854
                     /gene="ypdB"
                     /locus_tag="OO433_001059"
     CDS             21120..21854
                     /gene="ypdB"
                     /locus_tag="OO433_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6049612.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            21867..22724
                     /locus_tag="OO433_001060"
     CDS             21867..22724
                     /locus_tag="OO433_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6049613.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNSQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYTQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(22727..25222)
                     /gene="ptsP"
                     /locus_tag="OO433_001061"
     CDS             complement(22727..25222)
                     /gene="ptsP"
                     /locus_tag="OO433_001061"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6049614.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQAQRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASSVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRESDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(25247..26284)
                     /gene="ypdE"
                     /locus_tag="OO433_001062"
     CDS             complement(25247..26284)
                     /gene="ypdE"
                     /locus_tag="OO433_001062"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6049615.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGTRSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGVRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQICNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIKRLT
                     RETVVQLTDFR"
     gene            complement(26284..27369)
                     /gene="ypdF"
                     /locus_tag="OO433_001063"
     CDS             complement(26284..27369)
                     /gene="ypdF"
                     /locus_tag="OO433_001063"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6049616.1"
                     /translation="MTLLASLRDWLKEQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSSYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGVLPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(27384..28631)
                     /locus_tag="OO433_001064"
     CDS             complement(27384..28631)
                     /locus_tag="OO433_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6049617.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGVIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(28653..28979)
                     /locus_tag="OO433_001065"
     CDS             complement(28653..28979)
                     /locus_tag="OO433_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6049618.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(29198..30163)
                     /gene="glk"
                     /locus_tag="OO433_001066"
     CDS             complement(29198..30163)
                     /gene="glk"
                     /locus_tag="OO433_001066"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6049619.1"
                     /translation="MIKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            30367..31623
                     /locus_tag="OO433_001067"
     CDS             30367..31623
                     /locus_tag="OO433_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6049620.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTINIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            31738..32064
                     /locus_tag="OO433_001068"
     CDS             31738..32064
                     /locus_tag="OO433_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6049621.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERSYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(32205..33443)
                     /locus_tag="OO433_001069"
     CDS             complement(32205..33443)
                     /locus_tag="OO433_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6049622.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKTLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVLPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            33779..34981
                     /gene="nupC"
                     /locus_tag="OO433_001070"
     CDS             33779..34981
                     /gene="nupC"
                     /locus_tag="OO433_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6049623.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT         9231 a         8692 c         8082 g         9000 t
ORIGIN      
        1 gtcacggcga actgcctttc aaggttgaag tggcacgacc tcattggctg aaggtttacc
       61 ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc
      121 accctatctg gcaaaagcat ttgctgaaac tgctgccact ggggggatta ttgaatcaga
      181 actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc aaccgatcag
      241 cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccagctcca taaaagcacg
      301 cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag cggggttgct
      361 gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca
      421 atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc
      481 ccgcattggc tttaaggtga cagttcatat gtctgctgat gcccgggcat ggaaaaaagc
      541 gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgccgt
      601 cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg atgacgaaaa
      661 ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag cgcaatttgc
      721 ccagcaaggc cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt
      781 tggcggtggt cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg atcatgttca
      841 ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt
      901 acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct
      961 tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gtgcgtctgc tggatggctt
     1021 ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg
     1081 tattcgtctt gaaccttcgg cactggcggg tatggccgga cctcagcgcg tgtgtgcatc
     1141 agtaagttac caacagatgc acggtttcag cgcagaacaa ctgcgtaata ccactcatct
     1201 ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat atctggcaaa
     1261 aggccgttaa taacgtttca acgcagcatc gcaatccttt ccctgggtga gcgatgctgc
     1321 cgatggcgca gacttaagat ccccggtctt acccgctata accccctcct tttatcatca
     1381 cccaacgcct ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg taagtaagat
     1441 aaaagcaata gccgccatac ggaaaatctc atttgcagaa atagaaagtg actgctgggt
     1501 tatttcatta ttaatttcat taagaactcc tgaaagcgaa ccataatatt tatccataat
     1561 ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg
     1621 gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga
     1681 caaggtacga aaaaaattac tcatactcga ggcattggca aatttattat ctggcaagcc
     1741 tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca acggcgaatc cctgaaaaaa
     1801 ctgcggcaaa atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta
     1861 atagcaaacc gcatacatca aaaaactaaa tgtcactaac aaccgcatgt ctattttgtt
     1921 gccataacgt cctatcaaag gtgaaattaa tagtggcatg atgccgatgg gcgcataagc
     1981 aagtccggcc catatcgcat tataccccat cgtttcctgg agtaactgcg gcataaggac
     2041 gatcgctcca gagtaaaata aatacgcgca tgtgatactc acaataccaa tggtgaagtt
     2101 acgggactta aacaaactga gatcaagaat cgggttctct gaggtcgact cccaaatgac
     2161 taaagagatc agagaaataa ctgatactac tgttaatatt attattgtac tcgagttgaa
     2221 ccaatccaga tcgcgccctt tatcaagcat aatttgcaag ccaccaacac cgagcactaa
     2281 cagggtcagt cctggtagat tcattttgac cggtgaagtc tcagtttctc ttcctttaag
     2341 taaggttaag cataatgtca ggacgataat ccccataggg acattgatta aaaatatcca
     2401 accccagcta aagttatcac aaatataacc gcccaatatc ggcccacata tcggagcgat
     2461 aatcacggtc attgaccata atgccagagc aaatgttctt ttttctggcg gataattcct
     2521 taataataaa ctctgtgaca gtggaattaa cggccccgcc attaacccct gaacgactct
     2581 aaaaaatatc agcacatcaa gattggtcga taggctacac attaatgaag acagcgaaaa
     2641 aaaggttact gaaagtaaaa ataatcttaa ttcgcctatt ctttgtgcca acctgccagt
     2701 aacagggatc gcaatggcat ttgctacacc aaacgaggtg ataacccagg tgccttcgtc
     2761 tgttgatgct cccagaaagc cagatattgt cggtattgcg acgttagaaa tagtggaatc
     2821 caacatttgc ataaatgtcg ctaatgacaa tgcaatagtg acgcaccata acgtcccacc
     2881 ggttaatggt gccggagtcg atttagtgat tgccattaaa gttgtccatt atgcgaaata
     2941 atgttgctaa tttctttttc tatcggactg gtatcgataa ctaaagcctt actggtataa
     3001 gccggcatgg aggtcacggt tgaagccagc tcaggcatct cggcaatgtc ttcgttcttc
     3061 gtatcaatag ttgctgtcat cgataaacca atacgcaagg gatgttccat gagttctttt
     3121 ggatcaagag aaacttcaac cggtacacgc tgaacgattt tgatccagtt ccctgtcgca
     3181 ttttgtgcag gtaataagga gaacgcattg ccggttccca tattgatccc tgtcacccga
     3241 ccatgaaaca caacattttc accataaaga tcgctgataa tattgaccga ttgaccaatc
     3301 cgcacatccg tgagttgtgt ttctttaaag ttggcattaa cccacatttg acgtgccggt
     3361 acgacagcca ttaacgattg tccggggctc actgtttcgc cgacctgaac acttctctgg
     3421 gcaatatagc cggtaaccgg actcttaata tccgtacgtt taagcgccaa ccaggcttct
     3481 ttagttgcat ccgccgcttc aacgacttgt ggctgacggt ttaatggtgt gttcattact
     3541 aaagctttat tcgctttata agcctggata gcggcattca atgccgcttt gctacttatt
     3601 aacgtatctt tggtatgctc cagcgtttct tttgaaataa ccccctgctt cgctaacggc
     3661 actcgacggt tataatcttc taaagattgt tgatactgaa tacgtgctga agcgacttcg
     3721 gcactgtatt gtttatcctg taagtatagt ttattcgttt gccgaacaat atttgccaga
     3781 ttatttttag ctttattgag tgcgatagtg gcatcagttt tatccagtga aactaaaatg
     3841 tcaccttgtc gaacgtagtt cgtatcttta tgattaacga cagtgacact acctgagact
     3901 tgtgcagaaa ttggatctgc attccccgtg acataggcgt catctgtact aatcatgtct
     3961 tctaattcca ttgaccaata gacataggca cctgaaaacg caataaataa aactaccgcc
     4021 aataaagaaa agtattttct tctgttagaa tgttttttat ttgaattaat ctgttccact
     4081 attatctctc atttctcata gatgaaattt atgaattgaa tgaaaagagc attcattctt
     4141 catatcaaac acagtgcatg tgttgcacat tatcccagga acgttggagg cctaatttaa
     4201 atgaggtgca aaaaaaacaa cagaggacta aaccgtggct tttgcaatac aattcttacg
     4261 cctgtaggat tagtaagaag acttatagtg ctaacttgaa actataaatc atcggtacaa
     4321 cccctgattt tattgttgac atttcattta tgccgactat ttatatggta tacttgtcga
     4381 attatcttaa aggaagctca gattttctta tttttattga gaaaatgaga tgacgcctta
     4441 tgtctgtatt actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac
     4501 gcaataatta ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa
     4561 aacgatattg aaatcttagc ggagttgact gaaggggcaa gcgccgttca gcgggtggaa
     4621 acacttaagc ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag
     4681 gtgttagaaa cgctgaggaa gcgccaatat agcggaatta ttattattgt ctccgctaaa
     4741 aatgaccatt tttacgggaa acattgtgct gatgctggcg ctaatggatt cgtgagtaaa
     4801 aaagaaggca tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat
     4861 ttccccttct ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac
     4921 tccttatcga aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat
     4981 gacattgctg aaaaaatgtt catcagcaac aaaactgtca gcacttataa aagtcgcctg
     5041 atggaaaaat tagaatgtaa atcactgatg gatctttaca cattcgcgca acgtaacaaa
     5101 atcggctaat cacatgaagt ttttacccta tttttttctt ctctgttgtg gtctttggtc
     5161 gaccataagt ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg
     5221 tgtcacactt gatccagtac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa
     5281 aaatcttgtg attgcagtac acaagtccca aacggctacc ttgttgcata ccgattcgca
     5341 gcaacgggtt cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat
     5401 caaattaaca ctccgggaat acgccgatca tcaaaaagca atggacgcgc tggaagaagg
     5461 tgaagtcgat atagtgttat cacatttagt tgcttcgccg cctcttaatg atgacattgc
     5521 tgcaaccaaa ccactgataa ttacctttcc ggcgctcgtc accacccttc acgattcaat
     5581 gcgaccgctt acctcatcaa aaccagtaaa tattgctcga gtagcaaatt atccccctga
     5641 cgaggtaatt catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca
     5701 ggcattagca tccgtctcag ccggacagaa tgattacttt attggtagta acatcattac
     5761 cagcagtatg atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc
     5821 accgcgtcaa tataattttt tcttgaccag aaaagaatct gtcgttctta atgaagtact
     5881 caatcgattt gttgatgctt taacaaatga agttcgctat gaagtttcac aaaattggct
     5941 tgatacagga aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca
     6001 gtggattaag caacatcccg atttaaaggt gctggaaaat ccttactctc ctccctattc
     6061 tatgacggat gaaaatggct cggttcgggg cgttatgggg gatattctta atattattac
     6121 cttgcaaaca ggtttaaatt tttctccgat caccgtttca cacaatatcc atgccggaac
     6181 acagcttaac cccggtggat gggatataat acctggcgct atttatagtg aagatcgaga
     6241 aaataatgtt tcatttgcag aagctttcat aacaacgcca tacgtttttg tcatgcaaaa
     6301 agcacctgac agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta
     6361 tgagctgcat tcgcaattaa aagagatgta tccggaggtt gagtggataa aagtcgataa
     6421 cgccagcgct gcatttcaca aggtcaagga aggtgaactt gatgctctgg tcgcgacaca
     6481 gctaaattcg cgttacatga tcgatcatta ctatcctaat gaactgtatc attttcttat
     6541 tcccggcgtt ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa
     6601 ggatattatt aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga
     6661 aaaatggatt aaaatgccca atgtgaccat tgacacatgg gacctctata gcgagcaatt
     6721 ttatattgtt acgacattat ccgttttatt agttggcagt agccttttat ggggattcta
     6781 cctgttacgc tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc
     6841 attccgaaaa gcactctcgg attccttacc aaatccaact tatgttgtaa actggcaagg
     6901 taatgtcatt agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa
     6961 tgcaatgtta ccattagaaa acagtgactc accctttaaa gatgtttttt ctaatgcgca
     7021 tgaagtcaca gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga
     7081 taatggcatc gagaaaagat gcattaatca ctggcataca ttatgcaatc tgccagccag
     7141 cgatcatgct gtttatattt gtggttggca agatattacc gagacgcgtg atttaattca
     7201 tgcactcgaa gtagagagaa ataaagcgat caatgcaact gtcgcaaaaa gtcagtttct
     7261 ggcaacaatg agtcacgaaa taagaacacc aataagctcc attatgggct tcctggaact
     7321 tctgtcgggt tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc
     7381 caccggacaa tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc
     7441 gggtaactat caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg
     7501 tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacatt
     7561 tcctgatcat tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt
     7621 actgagcaat gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggt
     7681 tcacattgat gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt
     7741 atcgcaggaa gaacaacaac aactctttaa acgctacagc caaacaagtg caggtcgcca
     7801 gcaaacagga tctggcttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg
     7861 cgatttgtca ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt
     7921 agaaattagc cagcaagtgg cgactgtcga ggcaaaagca gaacacccca tcacactacc
     7981 tgaaaagttg agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg
     8041 ccagctaaat ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca
     8101 caaagtcagt atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga
     8161 tggttttgag ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct
     8221 tacagccaac gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg
     8281 tttgttcaaa ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt
     8341 tgcgcatatt gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc
     8401 gaacgatcta caactgatgc aggagattct catcactttc cagcatgaaa cgcataaaga
     8461 tctacccgct gcgtttcaag cactagaagc tggcgataac agaactttcc atcagtgtat
     8521 tcatcgcatc cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca
     8581 tcagttagaa ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa
     8641 ctctgtaaaa gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa
     8701 tgactaaata gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac
     8761 atttatgatg agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc
     8821 ggcataacat gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt
     8881 cccccggctt caatcaacat atttctcgcc tgagtttgtg gcaatttaat ggcctcagcc
     8941 acacttaata acggcgcgac gggtacacca acttcatgta ttctggctaa ccaaacttca
     9001 gctgcctgcg tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg
     9061 cgtaaaatat tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg
     9121 cataacgcag aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta
     9181 ttgaaaacat caaaaggtgc catgtaggga tggcgatttc ccagacgttg tggtgacttc
     9241 cctgtcgcga tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc
     9301 gctatatcga catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt
     9361 ccgctgaata aatagacacc gccgcataga tccgcaagag atgtaccaac gcgcactggc
     9421 ggagcatcag gatatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg
     9481 taggcaggag catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg
     9541 aggcgcgggt tgatttcttg aagcgtttcc catgaaaacc ccagtttttc cattgtacct
     9601 gggcgaaaat tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta
     9661 tcgtaatcat tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa
     9721 tagagtgact gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc
     9781 ggctcaactt taattaccct tgcgcccata ttacaaagaa gttgagttcc gaaaggtcca
     9841 ttaaggacat gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta
     9901 tttgtcattt gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg
     9961 taaaatcaat aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc
    10021 cgtgacgaca aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac
    10081 attaaaccgg ctggcaataa taattccgga gaatgccggc ggtaatgcgc ctgccagtac
    10141 catcatctgc agatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagtgccag
    10201 tggcatcaga atcagcttca ggaaggtgtt ataagcaatt tcggcactga actcgaattt
    10261 atgtgcagcc agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat
    10321 cagattaaag gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt
    10381 tgccagaaca ggtgcccata ctactggctc ctttgccgca gaaattaatg cactcagatt
    10441 actattcttc ttaccatccg ctcctgaaga aggattcagc aaatacagac caataggaat
    10501 agtaattgcg ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc
    10561 accataaata ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacctgcaat
    10621 taatgcacat acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccgaacca
    10681 ggagaaaaag aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc
    10741 aaaaatcatt tccctgtttg cccgagtaat agatacaaat agagccgcag gaagcgcgta
    10801 gtttaatacc agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc
    10861 gctaaaataa cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt
    10921 tagcataata gtatttccga taagggtccc ctcaccatcg cataatgagt tggcaagagg
    10981 agacgatgtc agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc
    11041 actttcagtt cctgctgcag ggtcgatgac cacattaata atggtcggtt tgcgcgactg
    11101 aataccggtg gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc
    11161 acgaaacgca tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggagatgg
    11221 tgcaccagcg ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat
    11281 aacgatcgtc accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc
    11341 aaaagcacta tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat
    11401 ggcatagccc ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg
    11461 tttatacata tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa
    11521 ataaatatcc tggttctcgc gcaatacgtc gcgcacagaa cttaatgcat taaagtaatt
    11581 taatggttga gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg
    11641 gatatttaaa atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc
    11701 cagcatacct tgcatactgg atgcaatatc accaatgact ggcacagcaa tggggcggtt
    11761 gctgtcaatt tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc
    11821 ttttttaccg tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc
    11881 atttgccagg gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat
    11941 ccctttcgcc atagacattg gcaggaatgg aatctgagca ctttcaataa attcacgaag
    12001 ctgttcatca gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc
    12061 ttttgctaaa agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga
    12121 cggattttca actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag
    12181 gacatttgct ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat
    12241 agcgcgtgcc aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc
    12301 atacggtttt gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac
    12361 gatcgcgcgg tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt
    12421 ggccaatgcc gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg
    12481 tttttgggta agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa
    12541 accaatataa cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc
    12601 tacaacacca taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat
    12661 accatctgtc atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg
    12721 ggagccagaa gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca
    12781 tcgtcgctat aacctagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca
    12841 gctttaatat ccggcgtaaa agcagagaat ttcattggac aaccaacggt cagatatttt
    12901 ccacgcaacg gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt
    12961 gaaatttctt tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc
    13021 accgcttcat gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa
    13081 atatgtggct gtcgggcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg
    13141 ccgatggctt tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg
    13201 ttcggatcgg tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct
    13261 gcattaccac cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct
    13321 tccagataac ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc
    13381 aatacggcat cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc
    13441 agcaaagcag caagcaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct
    13501 gcagcgctta ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct
    13561 gcctgagcaa cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct
    13621 ttgatcgaac caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc
    13681 atgtgatcaa tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc
    13741 ttttccatta cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta
    13801 ctgttaagca tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtgacgc
    13861 gttacgtcac caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc
    13921 atttgagtac aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga
    13981 agtggagttg acatagcaat acctattagt taattaaata tgtgttaatt tagcgtttat
    14041 ttaaggcgta atatcttcta taaataaccc gcaaaatatc gtcgtagctg cactcgatat
    14101 ttttaaaatc ctgtatcaag tgctgaaaat catttcatcg ccacttccaa ttcctttctg
    14161 catggtaagt agtgaggacg aaaagaagaa tatgataaat attgattcgt ggtaatcaga
    14221 gagagtcgcg acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa
    14281 gtaagggtat gattacgctt tttaaagcgt accataaaac ataacataat aaatgatttg
    14341 tgatgacacg ggattattgg ggtttacttt gaggaagata aaatgcgtgt ttatagaacc
    14401 gcgagcgcca aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg
    14461 gctggaggga gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca
    14521 gtgcttcgct gtcgacgatg attccatctt ccgcaccttt cagcgccagg tttgcctcgt
    14581 agtattgctt tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa
    14641 gcggcatcag atattggttt tccattacgc caacaccggc caggcgaagt tcttccattg
    14701 cgcccttttt atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt
    14761 cgggagttgc tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt
    14821 catcattgag attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat
    14881 ctgacagcaa agcggaagct tcattggtca gttcttttgc cttttctgga tcgccatgaa
    14941 acagagccag gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac
    15001 gctctgctgc tgccgttttc tgggtttgct gaacttgctg agtttgctgt tgtgccgcta
    15061 ccggtgcatt atcagcagcc catacagttg aagatgccag aattgctgtg accatcgtag
    15121 ccataattaa acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc
    15181 atattgggtg agcatgaaat gagaaatcag agagcaacgg tcattttgaa agggttatct
    15241 tacagttgta gttattatcg caagaagtat tttagtctta caattgtagg aatctgcttc
    15301 ctgtcgcaga gaactattct tatgtaccac aaagagatgt catttattat atcgatgtaa
    15361 atattgatta attatatgta aatacagcca taacatcgta ggttctcagt gaaccatttt
    15421 caggataact ttctatgaaa gtaaacttaa tacttttcag cttattttta ttggtctata
    15481 ttatggcatg caatgttttt gcattttcca tttctggtgg tgtaagtgag aggagctata
    15541 aagagactga aaaaacatca gcgatgacga ccacacactc tacaaaactt cagccatcac
    15601 aggcgatttt gtttaagatg agagaagatg cgccaccatt aaacctcaca gaagaaatca
    15661 cgcctactta tccgacaaag gcgaattatc ttattcatcc tgtgcgatag cgtctgtcac
    15721 atggcaggtg aacaatcatg tctgatgctg atgtgaacac ctgcccaaat gagccatcct
    15781 gacccactat cgcctgccat accccaccat aaagcgaatc atcaagccac aagccggggc
    15841 aagaaacata aatatctccg tcactagttc attaccgcca gttaaccaca tggtgaagta
    15901 ataaatgagt gcagcgagga tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt
    15961 cagtacctga ccgatgtagc cgcttacgca gacaatacac agggcgagaa acatccataa
    16021 ataaatcata gctcaacctc ctgccgcgct aaaaaacctg taacttcagt cactttaata
    16081 gtaagacaat ccttaacctc tggcaatttt tgctaagcat caatgaaaac agcatgttaa
    16141 atgcaagact gttgtgtacg gaaaaatatt tactttgcac gattaataat cagtagctga
    16201 aagcagtcag cgagtgataa agtacagata ccgcattcat gctcctcgtg gttatgtcct
    16261 gactagtctt tacacacttt acaggaacca ttgtcgtaca tgatggccca accaattgag
    16321 tgtaccgctg tcgacatgga atcttctgcg atacaacaat tcgtatctac agaaggtaac
    16381 tatgtttcca caatgcaaat tttcccgcga gtttctacat cctcgctact ggctcacatg
    16441 gtttgggctt ggtgtactct ggctttgggt acagcttcct tatcctgttc tctgctttct
    16501 cggcacgcgt attggcgcaa tggcgcgacc attcctgaaa cgtcgtgaat ctatcgcccg
    16561 taaaaacctg gaactttgtt tcccgcagca ttctgcggaa gaacgcgaga agatgattgc
    16621 cgaaaacttt cgttcactcg gcatggcgct ggtagaaacc ggcatggcat ggttctggcc
    16681 cgacagtcgc gtacgtaaat ggtttgatgt tgaagggttg gataacctta aacgcgcaca
    16741 aatgcaaaat cgcggcgtaa tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg
    16801 ccgcgtgatg ggactgtgcc aaccaatgat ggctacctat cgtccacata ataatcagct
    16861 gatggaatgg gtgcagaccc gtgggcgcat gcgctctaac aaagcgatga tcggcagaaa
    16921 taatctgcgc ggcattgtcg gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga
    16981 tcaggattat ggtcgtaaag gcagctcctt cgcgccgttc tttgcggtgg aaaatgtcgc
    17041 cacaaccaat ggcacctatg ttctctcccg tctctctggc gcagccatgt tgaccgtaac
    17101 gatggtaaga aaagcggatt acagcggata tcgtttgttc atcaccccag agatggaagg
    17161 ctacccgaca gatgaaaatc aagccgctgc ctatatgaac aagattatcg agaaagagat
    17221 catgcgcgca ccggagcagt acctctggat ccactgtcgc tttaaaacgc gcccggtggg
    17281 agaatcgtcg ttgtacattt aacaaactgc tcttactctt tccctcctca aatcggccat
    17341 aataacctca tggtttttat ggcttttttc ttaaggtttt ttccttcgct gaatatctaa
    17401 ttattaccgt cagaaattaa actgtcgccg atatgagaca cttgtaagtt actaaaagta
    17461 aataagaaaa tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc cgtctcgagc
    17521 agtttagctc agaattctgg ggtgatgccc acggtaaaat acagggcttt taaggtcgct
    17581 ttgcccagtg tcaactcact gtatcaggtg taatgaagtc attcaggcgt aacagcaatt
    17641 acgcggagag atgtaaagtg aaatatttct ttatgggcat ttcttttatg gtcatcgttt
    17701 gggccggtac ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa acaggagcca
    17761 tcaggctcct gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt gaatatgttt
    17821 gctgctggcg ggtagtaaac cgtcggcacg gaacatcgct ttaatccctc tgatggcctg
    17881 acgaatacgg tcgcggtttt caatcagtgc aaagcgaaca tgcgtatcac catagtcgcc
    17941 aaagccaatc cccggcgaga cacagacctt cgcttcgtta agcaatttct tggcaaatac
    18001 cagcgagccc atggcagcat acggttccgg tattttcgcc cagacataca tcgaagcctt
    18061 cggcatttca actatccagc ccgcttcatg cagcccttta accaatacat cacggcggcg
    18121 tttgtactgt tcggcaatgt cgcgcacgca ctgttgatcg ccctccagcg ccgcaatcgc
    18181 cgccacctgc aacggcgtaa aggtgccgta atcgtgatag cttttaatac gagccagggc
    18241 gctgaccagc gttttgttgc caaccataaa gccgatacgc cagcccgcca tgttgtagct
    18301 tttcgacagc gtaaagaact cgactgccac atcgcgtgca cccggtacct gcatgattga
    18361 cggcgctttc cagccatcgt agacgatatc ggcataggcc aggtcgtgga ccaccagcac
    18421 atcgtagcgt ttcgccagcg ccacaacctt ttcgaagaac tccagctcca cgcattgcgc
    18481 ggttggattc gacgggaagc cgaggatcat catcttcggt ttcggataac tttcacgaat
    18541 ggcgcgctcc agttcgttga agaaatcgac accttccacc agcggcactg agcgtacctg
    18601 cgccccggca atcaccgcgc cataaatatg gatcgggtaa cttggattcg gcaccagcac
    18661 cgtgtcacca tgatccagcg tcgccagcat cagatgcgcc aggccctctt tcgaaccaat
    18721 agtgacgatg gcttctgatt ccgggtcgat ttcaacgtcg tagcgatcct gataccagcg
    18781 ggaaatggcg cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat gcgtgtcctg
    18841 gcgctgggcc acagtacata atttttcgac gatatgcggc ggagtcgcac cgtccgggtt
    18901 acccatgctg aaatcgataa tatcttcgcc gcgccgacgc gcagccattt tcagttcagc
    18961 ggtgatgtta aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg
    19021 agtgtcagcc ataatttcct cagattaacg ttagcgcccg gaccgtccga gcgacgctgc
    19081 cacaatggtg gctgttttga aaatagcctg attaatttct gtctgtccag cgttttagaa
    19141 catttttatc aatggcttta aatgaggaac tccacttcaa aaacaggaaa tttttctacg
    19201 ctaacaagaa aattaccacc aattttatta acattataat ttaaataaat ttaccagcca
    19261 gaagtgcatt atcaagagat acattgctaa tacaccattt atctttcaat ccccttttaa
    19321 atttatggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt acctatcata
    19381 gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc tgctggcggt
    19441 cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta tccgcctgtt
    19501 tcgcgagctg ttgcacaagt cggcacactc cccaaaagag ttgcttgctg ttaccgccat
    19561 tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag gctcgctggt
    19621 gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt gggtaggcat
    19681 cattaccggc gtgattgcgg gtattcaccg gtatttaatt gatatcggcg gcgtgacggc
    19741 gatcccctgc tttatcacca gcattctggc gggttgtata tcaggctgga taaacctgaa
    19801 aatccccaaa gcacagcgct ggcgcgtcgg tattctcggc ggcatgttgt gtgagacgtt
    19861 gaccatgatt ctggtaattg tctgggcacc aactaccgcg ttggggatcg atatcgtctc
    19921 taaaatcggc attccaatga tcctcggtag cgtctgtatc ggctttattg tgcttctggt
    19981 gcaaagcgtt gagggcgaaa aagaggccag cgccgcgcgg caggccaagc tggcgctgga
    20041 tatcgccaac aaaacgctac cgctgtttcg ccatgtcaat agcgagtcat tacgcaaggt
    20101 ctgcgaaatt atccgcgatg acattcacgc cgatgcggtg gcgattacta ataccgatca
    20161 tgtgctggcc tatgttggcg tgggtgaaca taactatcag aatggcgatg acttcattag
    20221 cccgactacc cgtcaggcga tgaattacgg aaaaatcatc attaaaaaca atgatgaagc
    20281 ccaccgcaca ccagagattc attccatgct ggtgatccca ttgtgggaga aaggcgttgt
    20341 gaccggaacg ctgaaaattt actactgcca cgcgcatcag ataacctcgt cattacagga
    20401 aatggcggtt ggtctgtcgc aaatcatctc cacgcaactg gaggtttcac gcgccgagca
    20461 gctacgtgaa atggcaaata aggcagagct tcgcgccctg caaagcaaaa ttaatcccca
    20521 ttttctgttt aacgctctga acgctatttc atcgtcaatc cgtctgaatc cggataccgc
    20581 tcgccagttg atctttaatc tgtcgcgtta tctgcgctat aacattgaat taaaagacga
    20641 tgagcaaatc gatatcaaaa aagagctgta tcaaattaaa gactatattg ccattgagca
    20701 ggcccgcttt ggtgacaagc tgacggttat ctatgatatt gatgaagagg tgaattgctg
    20761 cattcccagc ctgctgatcc agccgttggt ggagaacgcc attgtccacg gtattcagcc
    20821 ttgcaaaggt aaaggcgttg tcaccatcag cgttgcagag tgcggaaatc gggtacgcat
    20881 tgcggtgcga gataccggca cggcatcgat ccaaaggtga ttgagcgggt cgaagcgaat
    20941 gaaatgccgg gcaataaaat tggcctgctg aatgtccatc atcgcgtgaa gttattgtat
    21001 ggcgaggggc tgcatatccg ccgcctggag ccggggacgg aaattgcgtt ttacattcct
    21061 aaccaacgca ccccagtcgc ctcacaggct acgttattgc tttgagccgg agtgatattg
    21121 tgaaagtcat cattgttgaa gacgaattcc tggcacaaca ggaactgagc tggctaatta
    21181 aagagcacag ccagatggag attgtcggca cctttgacga cggtctggac gtgttgaagt
    21241 ttttgcagca taaccgcgtc gacgccattt ttctggatat caatattccg tcgctggatg
    21301 gcgtgttgct ggcgcaaaac atcagccagt tcgcccataa accgtttatt gtgttcatca
    21361 ccgcgtggaa agaacatgcg gtagaagcgt ttgaactgga ggcgtttgac tacattctca
    21421 aaccgtatca ggagtcacgt attaccggga tgctgcaaaa actggaagcg gcctggcaac
    21481 aacagcagac cagcagtacg cctgccgcga cggtaacgcg tgagaatgac accattaatc
    21541 tggtgaaaga tgagcgaata atcgtcacgc caattaacga tatctattac gccgaagcgc
    21601 acgagaaaat gacctttgtc tatacgcggc gtgaatccta cgtaatgccg atgaacatta
    21661 ccgaattttg cagcaaactg ccgccgtcgc attttttccg ctgccatcgc tcattttgtg
    21721 tcaatctgaa caaaatacgc gaaatcgaac cgtggtttaa taacacctac attctgcgac
    21781 tgaaagatct ggattttgaa gtgccggtca gccgcagcaa agtgaaagaa tttcgccagt
    21841 taatgcatct ttaatcggga actttcatga aagcaccagg cttgcccgcc gatcaacaat
    21901 ttttcgccga tctgttcagc ggcctggtgc ttaactcgca actactgggg cgcgtctggt
    21961 ttgccagcca gcctgcctcg ttaccggtgg gcagtttatg tattgatttt ccccgtctgg
    22021 atatcgtgct gcgcggcgaa tacggcaacc tgctggaagc aaagcagcag cgaatggtgg
    22081 aaggagaaat gctgtttatt ccggcacgcg cggctaattt accgatcaac aacaaaccgg
    22141 tgatgctgtt aagcctggtg ttcgcaccga cctggcttgg gttatcgttt tacgatagcc
    22201 gcaccacgtc gttgttgcat cctgctcgcc agatccagct tcccagcctg caacgcggtg
    22261 aaggtgaagc gatgcttacc gccctcactc atcttagccg ttcgccgctg gagcagaata
    22321 tcattcagcc actggtgtta agtttgctgc atctttgccg caacgtggtg aatatgccgc
    22381 cgggtaattc gcaaccgcgc ggcgattttc tctatcacag catttgtaac tgggttcagg
    22441 ataattatac ccagccgctc acccgcgaga gcgtggcgca gttttttaat atcacgccca
    22501 atcatctgtc aaaactgttt gctcagcatg gaacgatgcg ttttatcgag tatgtgcgtt
    22561 gggtgcgaat ggcgaaggcg aggatgattt tgcagaaata tcatctgtca attcatgaag
    22621 tggcacagcg ttgcggtttt ccggatagcg actatttttg tcgcgttttc cggcgtcagt
    22681 ttggtctgac gccgggagag tacagcgccc gttttcaggg ctaacgtcag aaggttaatt
    22741 ctgtttccag cagcgtcagg atactttgtg catcttgcgc ggcaaacaac gactggcgga
    22801 agtttttatt caccagttta cgcgccagct gcgagaagac tttcacatga ttcatccctt
    22861 cgttagcacc cagcgtcagc atgatcacca gttcgacttc gcccatttct gactgccagt
    22921 caatcggttt cgccagccgg gcaatgctga tactggaatg acggatccac tgagatttgg
    22981 tgtgcgggat cgctacgcca aaaccaacgc cggtggtaac aatctcttcc cgctgccaga
    23041 catcttcttc cagctcaaac ggatgttcag tgcgcccgtt aacgccgagg ttgccgcaca
    23101 ggaactggat cgcctgctct ttattgctaa aatcctgatc aacaaagata ttctccagcg
    23161 ccagcagtgg gcgaacgtct tcttccggcg taaaggcggt gagtaacgct tcaatttcct
    23221 gcgcactgcg gcattcacat gcctgacgcg ccagttcccg acacgcctcg ctatccagtt
    23281 gacgaagctg gcttttcacc gccggaatac gcgggctact catactcagc tcatccaggc
    23341 ccagcccaag cagtagcggc agataacggc tttcaccgcc cagttcaccg caaatgccta
    23401 cccatttgcc ccgctgatgc gcagtggtaa ctatttgctg caacatgcgc aggaacgatg
    23461 gcgtaatcgg gttatatagc ggcgatacgc gcgggttatt acgatcgacc gcatacagat
    23521 actgggtcat atcgttggag ccgatactga agaaatcgac ctcatcgcag aagtggtcga
    23581 tgatgtagca caccgacggc acttccacca tgatcccaag cgtaatcgtc tctgcatgac
    23641 gcaggccatc gcgcttaagc tcaacgatcg ctttttggat ctcgcccttc acccataaga
    23701 tctgatcgag actgtggacc atcgggatca tcaactgggc gttgccaaaa ctggcggcgc
    23761 gcaaaatcgc ccgtagttga gtgcggaaca ggccagcaaa ttccgggtaa atacgtaccg
    23821 cgcgatagcc gaggaacggg ttctcttcct ggggaatatt cagataagga atgcttttat
    23881 cgccaccgat gtccattgtg cggaagataa tcggcttgtc gcccgctgcc agtagcacct
    23941 gctggtaggc ttcaaattgc tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca
    24001 tttcagtacg gaacagtcca acgccttccg cgccgttggc aaacgcgcct ggcgcttcca
    24061 gagcggtgcc gatattcgcc gcaatatcaa tacgcttgtt atcacgggaa taggccagct
    24121 gcgcggcggc ctgcgcctgt tgtttttggc gtttatccgc cagcgtctgt gcgacctgat
    24181 aataaccgct caccgcgtca ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca
    24241 ccgccggttg cccggcataa cgggcaatcg catccagcgg caggccgctc agtaccggga
    24301 tcgccgaggc gcgggccaga atcagggtat gcgaggtgcg cccagttttc tccagaatca
    24361 tgcctgcaag attttttaaa tcgaggctca aaaactggct tggggttaaa tcctcggcta
    24421 ccagaatagt cggtttttcc agcaccagat tgttgcgcgg cttcagttcc ggccaggtga
    24481 tatgcagcaa ctgttcgcta atatcgcgaa tgtcgctaac acgttcgcgc agataatcac
    24541 tggcagaagc agaaagtttg gcgcaaacct gctccatatt gctgatgatc gccgccccca
    24601 gcccttgatg ctgttctgtc atcagacgac ggatattgcc cgcgaattca tcatcctgaa
    24661 tcagcgacaa atgggcgcta aggatagttt tgctttcgcc gtcactctca cgcaattgct
    24721 ggttcagttg ctcggcaagc gttgcaaggc tgtgctccag ccgggtggaa tcttgcgcac
    24781 tggcggggat tgcccgataa ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca
    24841 cgccgacgct gcttgccagc acattgccgt acagtaaatc cgggttcagg cggcttaatg
    24901 aacgcggcag cggatgcgcc gtcagttctg cctgcgttgg ctggatgctg tcgctgtcga
    24961 taaagcgcac ctggatgtac tcttccaaca cgcgctgcgc ctgctcttca tcgctgccgc
    25021 taatgttcag gctgcaactg tcattaaata aagtaccggt gccaatcagc gccagcgagc
    25081 ttttcgcatc ggcctttgcg ttctggcgat ggttgataaa ggtgatttcg ctttgccact
    25141 ggctgcactg ttctttaagt tcccacgccg gacgagcatg aagaccgttt ggcaaaggac
    25201 agagaaattg aatcgttaac ataaccgctc cgggttaatc aggagatcat ctgaaatccg
    25261 tcagttgaac aaccgtctca cgcgtaagac gtttaataag ggcagataaa agctgctgca
    25321 tctgcaaaat gtcacggcaa tcggcaatcg atgcggcgca atgtccgtgg cgggttgctg
    25381 gccccatcac cactgtaggc acgccggtgc cggttaagtg caccgctccg ccgtccgtgc
    25441 cgccgttgct gaacatatct gcctgtaacg gcacgccaat ttctgccgcc acggtttcga
    25501 tccacgcggt aagttttggc ggcgcaatca gcgacttgtc gcttaacacc agcatcggcc
    25561 cgttacaaat ctgacgatgg ttagccgcgc cataatcaaa gtttttcgcc cagcaggcgg
    25621 tatcaagcac aatggcgaca tccggcgaca ccgcgcgggt ggcagtttgc ccgccgcgta
    25681 cccccacctc ttcgctggaa ctggccacca gccacacttc cgcaggcagt tcagcgctgt
    25741 gtagttcgcg cagtaacgtc accagcaggt agcaaccgag gcggtcatca aaggctttcc
    25801 ccatcacccg ctggtgaggg agaacctgaa aagttgtatc aaaggtgacg cgatcgcctg
    25861 gacgaattcc cgcctgcatc acttcgtcat acgagcgcgt accaatgtcc acgcgcatgg
    25921 cgctgacgtc attcccctgc cggtcgccgt caagcaggcc tggaattttg cactcttcac
    25981 gggtggtgat gcgcaccggc tgcagctggc gggcagccat gcgcacgttg ccaaccggca
    26041 gcacatcaat tgccccttcg cgggagatgc tgcgcaccat aaatcccact tcgtccatat
    26101 gggcgcagat catcaccttc ggccctgtcg attcattgag gcggatcagc accgatccca
    26161 gaccatcaaa tcgcacttct ttttgcaggc gatccgcttc ttccagcagg atctgccgca
    26221 cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat agcgataaat
    26281 ccattatgcc tctcccgtga gcaacactgt tttcggcatg gcgtagagca cttctgcgcc
    26341 ttgcggggta accagcacaa catcttcgat gcgcacgccc ccttgccctg gcaaataaat
    26401 ccccggctcc acggtcagca acatgcctgg ctgtagcgtc gtggtgtccc gcggtgaaaa
    26461 acgcggatct tcatggactt caatgccgat agcgtgacca gtgttatgac cgaaatagtc
    26521 gccaaatcca gcctcggtaa tgacccggcg cgcggcgtcg tcaatctgct ggcagcgcac
    26581 gccggggcga attgcagaga ttgccgcgag ctgtgcctgc aggacaatct gatagacgtc
    26641 aaacagcggg tgagattcgg cgctcacccc ttcgccattc accagcaagg tgcgcgtcat
    26701 atcagagcag tagccctgat acagcgcacc aaaatcgaga gtgacaaact cgcccgctgc
    26761 aacaatcttg tcgctggctt tgccgtgcgg cagcaccccg cgccagccgc tggcgacaat
    26821 ggtgtcaaaa gaggcttttt ctgcgccctg ctggcgcata aaccactcca gttcagcggc
    26881 tatctcgcgc tcgctcatcc ccgcctgaat aaagcggcga atatgctccg ctccgcgatc
    26941 ggcaatccca caggcgaggc ggattatctc cacctcctct ggcgttttga tttgccgcag
    27001 cacatccggc gtggcgctaa ccagtttcgc attgagttca gacttccagc ggtgcgcggt
    27061 ttcccaactc acctgctggc cttcaaaacc gagcgtttgc aactgctcat cggcaatgat
    27121 ttgattgacg atagtggtaa gcgtgttcgt cgcgtcaagc aaatgcagct ggtagccttg
    27181 cgcacgggct tccacctcaa cgaaatagct cgaatctacc agaatgtgcg cactttcacg
    27241 gctaataacc acatagcctg atccggtgga gatccccaga tgcggctgtt tgttctgccg
    27301 tgaggagaga agcactgcat ccagttgttg ctccttaagc cagtcgcgca gcgaagcgag
    27361 taatgtcatc tggattttcc ttcttacagg ctatcgatta acaatttgcc tttacggaac
    27421 atcatcagac gcaggaacac caccatcagc gcagttatga ctgcccccag cgcgatcccc
    27481 gccatataga cgccaaggtt agtgaccagc ggccatgccc agatagcgga ttccgggaac
    27541 cattgcactg cgcccagcca gacagcggcg gttgagccga caatcgcgcc gaccatatac
    27601 gacggaatgg cggtgatggg gctttccagc gcaaacggaa tcgcaccctc actaatcccc
    27661 ataaaggcaa ggaacatcgc ggtcttaccc tgcggataaa gttgagcgtt gaacaggcgt
    27721 ttgccggtta aacgacggtc aataatggtc gccagcccca gaccaatcgg aggaataacg
    27781 atagcgatag aacgagcggt gaccggtaat acgtggtcag tggtaaagct gaaggcaacg
    27841 aaacctgcgg ctttgttgat cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc
    27901 attgcgtaca tcagcgcgcc cttctcacct gcggcagtca gcacggtacg gataacgccg
    27961 ttgatccagc cgccaaaggg ggtaatgacg tagtacatcg ccagcatgac aaaaatggcg
    28021 gaaagaatcg gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg taactggatt
    28081 ttctggttca tccacttcac aaggtaaccc gcgacgatag aaataatcag cgcaccaatg
    28141 aaggtggacg gcaccggcga agaggtcgcc cattgcatag tgctgggatc gaagttcagc
    28201 agttgcgtcg gctgggtaga catcaagccg ccgataaaac cagccgggaa cgccagtttg
    28261 ccaccgatag agttcgccac aaaagcagca aacatcggaa tggcaaaacc aaacaacacg
    28321 ccgccgaagg actgcgacag ccaggcaaat ttcagtaagg agaggtcgaa accggagaat
    28381 ttcccgctat taagagcatc catgatgccg atctcagcag gaattttaag ccagctataa
    28441 gcaatcagct ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt
    28501 gaaatccctg acatcacatg ctgcgggagt tcaccccaga agctgctttt agaggcctgc
    28561 ggattcttaa ctgccgctgc cgcaccggat gcgccaggca caacggttgc actgcgtttt
    28621 ttaatggcca taacaattat ccctgtcgaa tattactgtt gttcagaggc aatcatctct
    28681 tcgatttctt tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa
    28741 acgtcgcgtg attcgaaacg ctcattatct tccggggtaa ctgccacgga gtggatgatg
    28801 atggttgctt cggcgatatc ctgcgccgtc aggcgattct ggataccgtc cgcgccctgg
    28861 gtttcaattt tcacttcata accggcttct accgccgctt cttccaacgc ctgagcggcc
    28921 ataaaggtgt gagccaggcc cattgggcag gcacataagg caatcagttt cttactcatc
    28981 gttaattcct cattcagtga tagttgacaa gtgaaattat gaacgtgcaa aacaaatcgc
    29041 cgttaccaga caaataatgc atttactgga gaattaatcc cgctgtacgg aagtgtgacg
    29101 caggtcggcc ttgtgagggg acacaaagaa aaattgaatt aactgtatga tttaaacgat
    29161 tatcgggaga gttacctccc gatataacag gaaggattta cagaatgtgc cctaaggtct
    29221 ggcgtaaatg tgcaccggaa ccgagaagac ccggattgtc atggacgatg agatacaccg
    29281 gaatatcatg gacatattct ttaaagcgcc ctttatcttc aaatgcagca cggaaaccag
    29341 aggctttgaa gaactcaagg aagcgcggca cgataccgcc cgcaataaac acgccgccaa
    29401 atgtcccgag attgagcgcc agattgccgc caaaacggcc cataatgacg caaaacagcg
    29461 acaacgcgcg gcggcaatcg gtacagctgt cagccagcgc gcgttcggta atatcttttg
    29521 gcttgagatt ttctggcagg cggttgtcag ctttcacaat tgcgcgatac aaattcacca
    29581 gcccagggcc agaaagcacg cgctccgccg aaacatgacc aatttccgca cgcaatattt
    29641 cgaggataat ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg ccttcgcccg
    29701 gcaagcttac ccaacgctta tcgacatgga ccagatgcgc aaccccaagc cctgttccgg
    29761 caccgtaaac cgcaataggt ttaccttcta ccggttctgc gccaccaaac tgaatcagat
    29821 gctctttttt cagcatcggg atcgccatcg atacagcggt aaaatcgtta ataatttcca
    29881 gatggctaaa accgagattc tttttcattt cggcaattga gaacgcccag gtatggttgg
    29941 tcattgccac ccagtcaccg gtaattgggc aagcgatggc aatacagccg tctttcacct
    30001 cgaccttatg ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc
    30061 ctgaataggt cttagcctgc gagatttcac cactggcaat atcacacaga gcaagacgtg
    30121 cgttggtgcc gcccacatca ccgactaatg catactttat cattcttcaa ctgctccgct
    30181 aaagtcaaaa taattctttc tcacactgta aatacctggg ggcataacaa caacgccgga
    30241 aaggcaggct ccctgtaaat atcgatctgg gtcacacatt tactttatcg tttcagcacc
    30301 aattgcagcg atgccttttt gcaagctggg caaactaagt atctgacccc gcataaggaa
    30361 tagaacatgc tccatccgcg agccagaacc atgttgttat tatcgctccc cgccgtggca
    30421 attgggattg cgtccagtct tattctgatt gtggtgatga aaatcgcctc ggtattacag
    30481 aatttgctct ggcaacgact gccgggaact ctggggatag cccaggattc acctctctgg
    30541 atcatcggtg tattaacgct aacgggtatt gcggtggggt tggttatccg tttcagccag
    30601 ggtcatgccg gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccagt tccgccctct
    30661 gcgctaccag gacttatcgt agcattaatt ctcggtcttg ctggcggcgt cagcctgggg
    30721 ccggaacatc cgatcatgac catcaatatc gcccttgccg tcgccattgg cgctcgtctg
    30781 ttaccgcgcg tcaaccgaat ggagtggact attttagcct ctgccggaac catcggcgcg
    30841 ctgtttggca cacctgttgc agcggcgttg atattttcgc aaaccttaaa tggcagtagt
    30901 gaagttccgc tatgggatcg tctctttgcg ccgttaatgg cggcagcagc tggtgcacta
    30961 actaccggat tatttttcca tccccatttt tcactgccca ttgcgcatta cggacagatg
    31021 gaaatgaccg atattctcag cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg
    31081 gtcgccgtat ggtgcttacc acggttgcac gcgatgatga atcaaatgaa aaatccggtg
    31141 ctcgtgctgg gtattggcgg atttattctc ggtattctgg gggttattgg tggaccagtt
    31201 tcgctgttta aagggctgga tgagatgcag cagatggtgg caaatcaggc tttcagcacc
    31261 agcgattact ttttgctggc ggtaattaaa cttgccgccc tggtcgttgc tgccgccagt
    31321 ggctttcgcg gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt agggttgatg
    31381 ctgcatgagc acgtacccgc cgtaccagcg gcaataaccg tttcttgcgc tattctcggc
    31441 atcgtgctgg tggtaacacg cgatggctgg ttaagtcttt ttatggcggc ggtcgttgta
    31501 cccaatacca cattgctacc gctgctctgt atcgtcatgc ttccggcatg gctgttatta
    31561 gcaggtaagc cgatgatgat ggtcaatcgt ccgaagcaac agccacccca cgataacgtt
    31621 tagcaaaaaa tgctctcctt tatgatttaa gagttatggc gatgatacgg ggctttacct
    31681 ccccgtaata ttgcgttaac aggccgctga cggcttcgat cgtgaaggag aataacaatg
    31741 ttcaggtcac tgtttctggc ggccgccctg atggcattta ccccgcttgc agcaaacgca
    31801 ggtgaaatca ccctactgcc atcaatcaaa ttacaaattg gcgatcgcga tcattacggt
    31861 aattactggg acggtggtca ctggcgcgac cgtgactact ggcatcgcaa ttatgagtgg
    31921 cgcaaaaacc gttggtggcg tcatgataat ggctatcatc gtggctggga taagcgtaaa
    31981 gcgtatgagc gtagctatcg tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc
    32041 cgcggacatg gacaccgcca ttaaacacat tcgccaatgg agcacaatgc ctgatgcgac
    32101 gctagcgcgt cttatcaggc ctacaaaccg gatctttaac gcgtagggcg gataaggcgt
    32161 ttacgccgca tccgccaata gtgccagatg cgacgctcat tcaactacaa tcccagcgcc
    32221 gtccccacca acaaccagat attcagcgcc acgaccagca ctacaatcac ccagcctgtc
    32281 tgttttacgc gtttgctgtt caccagatcg cccatcaact tgctgtcact ggtgaaaatc
    32341 agcagtggaa ccagcgccag ggcgatacca aaacttaaca gcacctgact cataaccaga
    32401 atccgtgtcg gatctaatcc catcagaatg acaataaatg acggcagcat ggtgactgta
    32461 cgacgcaccc acagcgggat atggaagcga atgaacccct gcatcaccac ctgccctgcc
    32521 agtgtcccca ctaccgttga ggacagtccg gcagcaacca gacttaaccc aaagaccgtt
    32581 gccgcagcat ggcttaacag cggttgcagc gtcagataag cctcatcaag atcggcaaca
    32641 ccagtatgac cagaaaagtg gaacgccgcc gcagctgtag ccatcatcgc cagattgaca
    32701 aaaccggcaa tagtcatggc gatagccaca tcccatttgg tggcggaata acgttgttga
    32761 cgcgaaccgc catgtaaatg ctgagtgagc gaggagtgca aataaatcac atgcggcata
    32821 atcgtcgccc ctaacacgcc tgctgccagg aagaccgctt ccgaagtagg taaactcggg
    32881 agcaccattc ctttacccag ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg
    32941 taagccgcgg caacaaacaa cagtaacccg ccaatcactt tctccagcgt tttttgcccg
    33001 cgacgttgca gcattaaaat caggaaagtc gcgatccccg tcagcaccgc gccctgcaac
    33061 aacgaaacac caagaatgag tttaaaaccg atcgccgcac caataaattc cgccaggtcg
    33121 gttgccatcg caataatttc tgcctgaacc caatagaacc acactacggg acgcggatag
    33181 tgatcgcgaa tctgctccgc cagattttta ccggtggcaa tccctagttt ggcagagagg
    33241 atctgaatca gcatcgccat caggttggcc caaacgacaa cccacagtag ctgatagccg
    33301 aagctggcac ccgcctgaat attggtcgca aagttaccgg gatcgatata accaatcgcc
    33361 gcaatgaacg caggtcccat taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta
    33421 ctctcaacgc gatagttcgt catcttgtgc ctctaaaaca tagcctttgc tatgtttcat
    33481 gctatgataa acgagaatga ttatcaaatt catttaaatg gattgtggtg atttctgtga
    33541 tagaccagga ttatgactac gaaaagattg atgacttgaa tgttatggtt atgtttaatg
    33601 ttagcacatt tacataactt tcagcttcca tacacaacat agcagaaatg tatgacagat
    33661 cactattttt gaagcctgtc acaggacgtc attatagtgt gtgtcagatc tcgttttcct
    33721 taaccatgtt acatagaatg tgcacggaaa tttaacctgc ctcatatttg gagcaaatat
    33781 ggaccgcgtc cttcattttg tactggcact tgccgttgtt gcgattctcg cactgctggt
    33841 aagcagcgac cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg ttatcgaagt
    33901 gttactggcg tggttcttcc tgaactccga cgttggttta ggcttcgtga aaggcttctc
    33961 cgaaatgttc gaaaaactgc tcggatttgc caacgaaggg actaacttcg tctttggtag
    34021 catgaatgat caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat
    34081 ctctgcactg atcggtattc tccagcacat tcgcgtgttg ccggtgatca tccgcgcaat
    34141 tggtttcctg ctctccaaag tcaacggcat gggcaaactg gaatccttta acgccgtcag
    34201 ctccctgatt ctgggtcagt ctgaaaactt tattgcctat aaagatatcc tcggcaaaat
    34261 ctcccgtaat cgtatgtaca ccatggctgc cacggcaatg tccaccgtgt cgatgtccat
    34321 cgttggtgca tacatgacca tgctggaacc gaaatacgtc gttgctgcgc tggtattgaa
    34381 catgttcagc acctttatcg tgctgtcgct gatcaaccct taccgtgttg atgccagtga
    34441 agaaaacatt cagatgtcca acctgcacga aggtcagagc ttcttcgaaa tgctgggtga
    34501 atacattctg gcaggtttca aagttgccat tatcgttgcc gcgatgctga tcggctttat
    34561 cgccctgatc gctgcgctga acgcactgtt tgccaccgtg actggctggt ttggctacag
    34621 catctccttc cagggcattc tgggctacat cttctatccg attgcatggg tgatgggtgt
    34681 tccttccagt gaagcactgc aagtgggcag tatcatggcg accaaactgg tttccaacga
    34741 gttcgttgcg atgatggatc tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg
    34801 catcatctct gtgttcctgg tttccttcgc taacttctct tcaatcggga ttatcgcagg
    34861 tgcagttaaa ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg gtctgaaact
    34921 ggtttacggc tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta
    34981 agaccataca taaaaaagcc gggga
//
LOCUS       DAJTSL010000025        34893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTSL010000025 DAJTSL010000000
VERSION     DAJTSL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.025"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..334)
                     /locus_tag="OO433_001071"
     CDS             complement(<1..334)
                     /locus_tag="OO433_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6049624.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGE"
     gene            complement(415..1842)
                     /locus_tag="OO433_001072"
     CDS             complement(415..1842)
                     /locus_tag="OO433_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6049625.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETEPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHLLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1839..2546)
                     /locus_tag="OO433_001073"
     CDS             complement(1839..2546)
                     /locus_tag="OO433_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6049626.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2709..3686
                     /locus_tag="OO433_001074"
     CDS             2709..3686
                     /locus_tag="OO433_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6049627.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKTALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3756..4238)
                     /locus_tag="OO433_001075"
     CDS             complement(3756..4238)
                     /locus_tag="OO433_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6049628.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4473..7055
                     /gene="leuS"
                     /locus_tag="OO433_001076"
     CDS             4473..7055
                     /gene="leuS"
                     /locus_tag="OO433_001076"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6049629.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKS
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7070..7651
                     /gene="lptE"
                     /locus_tag="OO433_001077"
     CDS             7070..7651
                     /gene="lptE"
                     /locus_tag="OO433_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6049630.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7651..8682
                     /gene="holA"
                     /locus_tag="OO433_001078"
     CDS             7651..8682
                     /gene="holA"
                     /locus_tag="OO433_001078"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6049631.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRTLHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8684..9325
                     /gene="nadD"
                     /locus_tag="OO433_001079"
     CDS             8684..9325
                     /gene="nadD"
                     /locus_tag="OO433_001079"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6049632.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNQED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9349..9957
                     /locus_tag="OO433_001080"
     CDS             9349..9957
                     /locus_tag="OO433_001080"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6049633.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPPLTARGIEQAQNLHTLLHGV
                     SFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAENY
                     SAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARLI
                     GMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVDNA"
     gene            10217..10534
                     /gene="rsfS"
                     /locus_tag="OO433_001081"
                     /gene_synonym="rsfA"
     CDS             10217..10534
                     /gene="rsfS"
                     /locus_tag="OO433_001081"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6049634.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10538..11005
                     /gene="rlmH"
                     /locus_tag="OO433_001082"
     CDS             10538..11005
                     /gene="rlmH"
                     /locus_tag="OO433_001082"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6049635.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11036..12937
                     /gene="mrdA"
                     /locus_tag="OO433_001083"
     CDS             11036..12937
                     /gene="mrdA"
                     /locus_tag="OO433_001083"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6049636.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTTHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12940..14052
                     /gene="mrdB"
                     /locus_tag="OO433_001084"
                     /gene_synonym="rodA"
     CDS             12940..14052
                     /gene="mrdB"
                     /locus_tag="OO433_001084"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6049637.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14063..15151
                     /gene="rlpA"
                     /locus_tag="OO433_001085"
     CDS             14063..15151
                     /gene="rlpA"
                     /locus_tag="OO433_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6049638.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15291..16502
                     /gene="dacA"
                     /locus_tag="OO433_001086"
     CDS             15291..16502
                     /gene="dacA"
                     /locus_tag="OO433_001086"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6049639.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDMYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16613..16876
                     /locus_tag="OO433_001087"
     CDS             16613..16876
                     /locus_tag="OO433_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6049640.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVEILYEELGKIDIVRMVL"
     gene            16977..17618
                     /gene="lipB"
                     /locus_tag="OO433_001088"
     CDS             16977..17618
                     /gene="lipB"
                     /locus_tag="OO433_001088"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6049641.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17877..18830
                     /locus_tag="OO433_001089"
     CDS             17877..18830
                     /locus_tag="OO433_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6049642.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIALNLLTIFEAVYVHK
                     GIVNAAKVLNLPPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNKEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19039..20004
                     /gene="lipA"
                     /locus_tag="OO433_001090"
     CDS             19039..20004
                     /gene="lipA"
                     /locus_tag="OO433_001090"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6049643.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVAIEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20105..20308)
                     /gene="tatE"
                     /locus_tag="OO433_001091"
     CDS             complement(20105..20308)
                     /gene="tatE"
                     /locus_tag="OO433_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6049644.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20437..21225)
                     /locus_tag="OO433_001092"
     CDS             complement(20437..21225)
                     /locus_tag="OO433_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6049645.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENAVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21318..21701
                     /gene="crcB"
                     /locus_tag="OO433_001093"
     CDS             21318..21701
                     /gene="crcB"
                     /locus_tag="OO433_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6049646.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21756..21965)
                     /gene="cspE"
                     /locus_tag="OO433_001094"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21756..21965)
                     /gene="cspE"
                     /locus_tag="OO433_001094"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6049647.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22140..22700)
                     /gene="pagP"
                     /locus_tag="OO433_001095"
     CDS             complement(22140..22700)
                     /gene="pagP"
                     /locus_tag="OO433_001095"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6049648.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAPTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23289..24674
                     /gene="dcuC"
                     /locus_tag="OO433_001096"
     CDS             23289..24674
                     /gene="dcuC"
                     /locus_tag="OO433_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6049649.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24715..25395)
                     /gene="dpiA"
                     /locus_tag="OO433_001097"
                     /gene_synonym="citB"
     CDS             complement(24715..25395)
                     /gene="dpiA"
                     /locus_tag="OO433_001097"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCR6049650.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(25364..27021)
                     /gene="dpiB"
                     /locus_tag="OO433_001098"
                     /pseudo
     CDS             complement(25364..27021)
                     /gene="dpiB"
                     /locus_tag="OO433_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            27370..28428
                     /gene="citC"
                     /locus_tag="OO433_001099"
     CDS             27370..28428
                     /gene="citC"
                     /locus_tag="OO433_001099"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6049651.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLKTPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            28443..28739
                     /gene="citD"
                     /locus_tag="OO433_001100"
     CDS             28443..28739
                     /gene="citD"
                     /locus_tag="OO433_001100"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6049652.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGTLDCILRARLEALLARASGIPTLPWEDCQ"
     gene            28736..29644
                     /gene="citE"
                     /locus_tag="OO433_001101"
     CDS             28736..29644
                     /gene="citE"
                     /locus_tag="OO433_001101"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6049653.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            29655..31187
                     /gene="citF"
                     /locus_tag="OO433_001102"
     CDS             29655..31187
                     /gene="citF"
                     /locus_tag="OO433_001102"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6049654.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIHRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQEQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPTR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            31191..31741
                     /gene="citX"
                     /locus_tag="OO433_001103"
                     /pseudo
     CDS             31191..31741
                     /gene="citX"
                     /locus_tag="OO433_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            31716..32593
                     /gene="citG"
                     /locus_tag="OO433_001104"
                     /pseudo
     CDS             31716..32593
                     /gene="citG"
                     /locus_tag="OO433_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
     gene            32644..34107
                     /gene="citT"
                     /locus_tag="OO433_001105"
     CDS             32644..34107
                     /gene="citT"
                     /locus_tag="OO433_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR6049655.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPNGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            34218..>34893
                     /gene="rna"
                     /locus_tag="OO433_001106"
     CDS             34218..>34893
                     /gene="rna"
                     /locus_tag="OO433_001106"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6049656.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPA"
BASE COUNT         8678 a         8896 c         8641 g         8678 t
ORIGIN      
        1 cttccccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
       61 agccaccagc aattaatgct ttccactctt caggggaaga gcgatgccat gtcagatata
      121 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
      181 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttata ccggtataca
      241 tttttggcga gtcatgcgcg ggtttgcaac cctgattaag tatttccatg ggcgcagaaa
      301 gcgcaaaaag aagatattgt tcttccttat tcatttgtta ataatccctt aagtgcatta
      361 actgactact tttttatatt acttgccggg ctttccgcac attaacgtcg actgtcaccc
      421 gaacagatgg agtattttcc ctatcaaacc ggcaaagata aaaatgtaga aaataatttt
      481 gatgacgccc agacagcccg gcccaccatc gtcaacgggc attggctgcg gttgcggctc
      541 cggttcaaca gtttgcttct cggtaaaatt cgccgcctga attaataatc gacgaataat
      601 taatgcttcc agtggacaac gatcttctgc atcaagacgg cttacgatcg gcattaatga
      661 ttcttctgtc ggttggctgg cctgcactaa ttgtttggca actaaatata atggccataa
      721 gagatgttct gtatcacgcg tgagtgagat ccgcggctta cgtcctttgt gcaaaaccat
      781 ctcgaccagt gaacgcccca gcgcagagag tttttcatcg ctgagtattt cgtataaatg
      841 aaaacgcgta cgggcgctgt cgccggggac ataaagtagc tgtttaccct gatcgttaac
      901 cagttcacga gcttcaatca tcatcaccac taacgggtaa taatcgaccc gatgctgacg
      961 gcaccattgc aatattaaag catccgcctg ggtggaggga tactcgcgcc agtagtcaca
     1021 aagttctgcc agcaagcttt cgccttctcc acaatagacg cgttgcgcgt attcataata
     1081 acgcgcatct tcattatcag gttcagcttc ccgccacgac cgggcgtaat ccagaagttc
     1141 ggggatatcc aggcgacctg cgctatacca gcggagtaaa tttctgtgca gttttgcatc
     1201 atcggggatg atcactgcgc gatgttgcgc taaatacaat gtcagccatt ctggatgcga
     1261 tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg tcattttgca cggcaacggg
     1321 cagatgcaga atggtctgga agttaaaaac attccccttc gcaatggcat ataaaaagga
     1381 ttccagttct tcttcgtcta tttccccgac gttttcctcc tgcaaccacc gcaatctttc
     1441 tgctaataac accacgcact cgaaggataa atgggcagtg ttcatggcga ttgtgcacag
     1501 cgaccagcgt aattcatcaa tctcttccat cgagcaataa tttaattgct gaatgaatcg
     1561 ctgccaggcg gagggcagaa aacgttcgct atcagaggca agtaacgcgc gaaaggcgag
     1621 cagaatttca tgttctgcta cctcatattc ttctggttgc caaacagatt tagcaggcga
     1681 ctgcgcaatc cgtagcgcct cctcatacgc ctgacgaagt tgtttaaaac cctgcggttc
     1741 ggtttctgga tgaaaggacg gtaacagcgc cagataagcg cggcggataa tatcgacgtc
     1801 agtcgtttcc tctatatcga ggatcttcca gcaatttttc ataagtcgct catatcctta
     1861 agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc aggacaaggt agggtaatac
     1921 gcatcatcac tggcaaataa ttgataaaac atatcattgc cggaatcgtc gtattcttta
     1981 cgcgctaaga attcgcgtaa taaaggcaga tgatcatctt cgggagactg ccaatattgc
     2041 cgaccaaagg tataaccggc aaagtattgc cgccagttac tataataacg tagagcccgt
     2101 agatgaatac gcgactggat ccatagactt tcttcttcac tcaaccagtt attaagtacg
     2161 cccattcgac ttaaaaaacc catccggaca taatcccatg ctttaattcc tccctcacca
     2221 cagcattcgg ctgtgctggc gacaaattgc gcataagcct gaccttgctc atttaattca
     2281 gcaagatagt cacgccattc acatggagaa taacgaaacc agcggtgata aaaccctgcc
     2341 aaccgagcgg catgaccgtt ctcgatcatt cggtgaattg tgcgcagcag gtcatcacgg
     2401 tcatcaattc cccatgagga agaaaggtca aaaaagcatg tacctaaata cattttgggg
     2461 gatgaatgcg aaggtaaaca gcattcattg cgtatttcca tcggagtgga aagtgcaaac
     2521 aatatttttt gcgaccccat gtccatataa tttatcctta aacattcatt taatgaatgt
     2581 aaactacatt tcgagactca gttatttata cttttctgac gcgacataat acgcataaaa
     2641 taaggcttac gcaaaaatgg aacacataaa atataatatg ttttatctac aaccataagg
     2701 aaatgataat gatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg
     2761 aacgtgctga aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg
     2821 atagcgcaat tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgccg
     2881 agcaaggtca ttgtgaagca caatattcct taggacaaaa gtatactgag gataaaagcc
     2941 gtcataaaga taatgagcat gccatctttt ggctgaaaaa aactgcccta caaggccata
     3001 ctttcgcttc caacgccctt ggctggatac tggatcgtgg agaagacccc aattataaag
     3061 aagcggttgt ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc
     3121 ttgggtggat gtacagaaat ggcaacggag tcgcaaaaga ctatgcgctg gcatttttct
     3181 ggtacaaaca agctgcatta caaggccata gtgacgcgca aaacaatctg gccgatcttt
     3241 atgaagacgg aaaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa
     3301 gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg
     3361 aaggggtgga ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg
     3421 aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga
     3481 aagattatca ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg
     3541 cctggtataa cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac
     3601 aggctctcga cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa
     3661 ttcgtgagac tgaagattta ctgtagagat aaaaacaagg gccggggaat ttcattcggc
     3721 ccatattctc tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg
     3781 aactggtcat gaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga
     3841 tggaagtgac atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa
     3901 accccatgat gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg
     3961 atatctgcca gctcctgcca taagctttct ttaatcaccc gcataaagac gctgtcagat
     4021 tcttctttca ggctctcttc atagctcatg gcgaactctt ccaggtcacg tcttacagct
     4081 cgcgtcagct catcgacctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc
     4141 gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gctcagtgac
     4201 gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta
     4261 actgactata ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg
     4321 taaattaatg tatgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt
     4381 tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg
     4441 tagccgtatt gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga
     4501 tagaatccaa agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg
     4561 agagcaaaga gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca
     4621 tgggccacgt acgtaactac accatcggtg acgtgatcgc ccgctaccag cgtatgctgg
     4681 gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg
     4741 cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa
     4801 accagctcaa aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc
     4861 cggaatacta ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat
     4921 ataagaagac ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac
     4981 aagttatcga cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc
     5041 agtggtttat caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg
     5101 atcactggcc agacaccgtt aaaagcatgc agcgtaactg gatcggtcgt tccgaaggcg
     5161 tggagatcac cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc
     5221 cggacacctt tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga
     5281 aagcggcgga aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag
     5341 ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg
     5401 ttcacccatt aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggagt
     5461 acggcacggg cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct
     5521 ctaaatacgg cctgaacatc aaaccagtta tcctggctgc tgacggctct gagccagatc
     5581 tttctcagca agccctgact gaaaaaggcg tgctgttcaa ctctggcgaa ttcaacggtc
     5641 ttgaccatga agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg
     5701 agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc tcgtcagcgt tactggggcg
     5761 cgccgattcc gatggtgacg ctggaagacg gtaccgtaat gccgaccccg gacgaccagc
     5821 tgccggtgat cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag
     5881 atccggagtg ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt
     5941 tcgacacctt tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag
     6001 aaggtatgct ggattccgaa gcagctaact actggctgcc ggtggatatc tacattggtg
     6061 gtatcgaaca cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg
     6121 atgcaggcat ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc
     6181 tggcagatgc cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg
     6241 atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc
     6301 atgaactggt ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc
     6361 cacaggtgat ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt
     6421 ctccggctga tatgactcta gaatggcagg aatccggtgt ggaaggggct aaccgctttc
     6481 tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa aggtgatgtt gcggcactga
     6541 acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaacgatcg
     6601 ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta
     6661 tggagctgat gaacaaactg gcgaaagcac caaccgatgg cgagcaggat cgcgctctga
     6721 tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc gttcaccccg cacatctgct
     6781 tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga caacgcgccg tggccggttg
     6841 ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag
     6901 tccgtgccaa aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg
     6961 gccaggaaca tctggtagca aaatatcttg atggcgttac cgtgcgtaaa gtgatttacg
     7021 taccaggtaa actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct
     7081 ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg
     7141 tgataccacg caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa
     7201 cgggccatta agccgtgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga
     7261 taaagaaacc acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa
     7321 agataccgca tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt
     7381 gaatgcgacc gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg
     7441 ttcgttcttt gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt
     7501 aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc
     7561 tgcggatatt cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc
     7621 tgcacgcgtc tccaccacgc tgggtaactg atgattcggt tgtacccgga acaactccgc
     7681 gcgcagctca atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta
     7741 ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg cacaaggatt cgaagaacac
     7801 cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct
     7861 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat
     7921 gcggcgatca atgaacaact tctcacactc accggacttc tgcatgacga cctgctgttg
     7981 atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgcgctt
     8041 gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg
     8101 gttgctgcgc gcgcaaaaca gctcaactta gaactggatg acgcggcaaa tcaggtgctc
     8161 tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg
     8221 ctctggccag acggcaaatt gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg
     8281 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcacattg
     8341 catattcttc agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta
     8401 caacgtgaac tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt
     8461 gcgttgtttg ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta
     8521 aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc
     8581 accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg
     8641 ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc
     8701 tctgtttggc ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct
     8761 ggcgaatttg attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg
     8821 tccccagccg gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccga
     8881 caagccatta tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc
     8941 gcaaacactg aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat
     9001 tggtcaggat tcactgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa
     9061 tgcacatttg atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata
     9121 ccagcaatgg ctggaagatc atttgacaca taaccaggaa gatcttcacc ttcagcctgc
     9181 cggtaaaatt tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga
     9241 acgtttgcaa aacggtgaat cgtgtgagga tttattgccg gaaccggtac tgacttacat
     9301 taaccaacaa ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg
     9361 ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc tttacagcgg tcatgcgccc
     9421 cccctgaccg cgcgcggtat tgagcaagcg caaaatctgc atacgctgct acatggtgtt
     9481 tcctttgatc tggttttatg cagtgaactg gaacgggcac agcataccgc gcgactggtt
     9541 ctcagtgacc gccagctccc cgtgcaaatc atccctgaac tcaacgaaat gttttttggc
     9601 gactgggaga tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg
     9661 tggtgcaatg actggcagca tgcaatcccc acgaacggtg aaggatttca ggcattttcg
     9721 caacgtgtgg aacgctttat cgcaaggctt agtgaatttc agcactatca gaatatttta
     9781 gtcgtcagcc atcagggtgt actgagtctg ttaatcgccc gtttaattgg catgcctgcc
     9841 gaggctatgt ggcattttcg cgttgaccag gggtgttgga gcgccattga tatcaaccaa
     9901 aaattcgcga cgctacgcgt cctcaatagc cgtgccatcg gggtcgataa tgcatgactt
     9961 ttctgttttt ttacgggtaa gccgcaacga ccattgacag acccgggcag gctgatattc
    10021 tccgcagcca gacttttttc gccagacacg actttgtaga aattgtttta caaaaatggc
    10081 gatgcaatct gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca
    10141 attgtcccga aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt
    10201 cacccagggg gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga
    10261 tgacctcaaa ggtcaggaca tcatcgcctt agacgttcaa ggcaaatcca gcatcaccga
    10321 ctgcatgatc atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt
    10381 tgtgcaggag tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc
    10441 cgactggatt gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg
    10501 tcgcctgtat gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt
    10561 gggaacgaaa atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc
    10621 gaaagatatg cccttcgagc tgattgaaat tccggccgga aaacgcggca agaatgcgga
    10681 catcaagcgc atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg
    10741 cattgtcacc ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct
    10801 ggaacgctgg aagctggatg gtcgcgacgt cagtctactg attggcgggc ctgaagggtt
    10861 gtcgcctgcc tgtaaagcgg cggcggagca gagctggtcg ctgtcggcgc ttaccctccc
    10921 ccatccgctg gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac
    10981 caaccatcct tatcaccgtg agtgataagg gagctttgag tagaaaacgc agcggatgaa
    11041 actacagaac tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct
    11101 ggtcgccttt ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct
    11161 gcaaattgtt cgctttaccg actaccagac ccgctctaat gaaaaccgca ttaagctggt
    11221 gcctatcgcg cccagccgcg gcattattta cgaccgtaat ggtatccctc tggccctcaa
    11281 ccgcactatc taccagatag aaatgatgcc agagaaagtc gataacgtgc agcaaacgct
    11341 ggacgctttg cgcagcgtgg ttgatctgac cgatgacgat attgctgcat tccgaaaaga
    11401 gcgcgcacgt tcacaccgtt tcacctctat tcccgtgaaa actaacctga ccgaagtaca
    11461 agtagctcgc tttgccgtca atcagtaccg ttttccgggg gttgaagtta aaggctataa
    11521 acgtcgttac tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat
    11581 caacgataaa gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac
    11641 gcatgatatc ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac
    11701 cggttatgaa gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt
    11761 accaccgcaa gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat
    11821 tgaaacgctg ctggcgggta gccgcgcagc tgtggtagtc accgatccgc gtacaggtgg
    11881 ggtgctggcg ctggtttcca cgcctagtta tgacccaaac ttgtttgttg acggtatctc
    11941 cagcaaagat tattccgcct tgttgaacga cccgaataca ccgctggtga accgcgccac
    12001 acagggggtt tatcctcccg cgtctacagt taaaccctat gtggcggttt cggcattgag
    12061 cgccggggtg atcacgcgca atacgacgct gtttgaccca ggctggtggc aactgccagg
    12121 ttcggaaaaa cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag
    12181 atcgctggaa gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga
    12241 tcgcctctcc gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc
    12301 ggaagaacgt tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc
    12361 gtggtatcag ggtgacacca ttccggttgg tatcggtcag ggttactgga cagcgacccc
    12421 aatccagatg agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca
    12481 tttgctgatg agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgcatgaacc
    12541 gcccgtcggc gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt
    12601 tgctaaccgc cctaacggta cgacgcataa atactttgcc agcgcaccgt acaaaattgc
    12661 ggcgaaatct ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca
    12721 caaaattgcc gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa
    12781 tccgcaagtg gctgtcgcca tgattctgga gaacggcggc gcgggtccgg cggttggtac
    12841 gctgatgcgt cagatcctcg accacattat gctgggtgat aacaacaccg atctgcctgc
    12901 ggaaaatcca gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa
    12961 aaaacattct gggataaagt ccatctcgat cccacaatgc tgctgatctt actggcattg
    13021 ctggtttaca gcgccctagt tatctggagc gccagcggtc aggatattgg catgatggag
    13081 cgtaaaatcg gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct
    13141 ccacgcgttt atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg
    13201 gcggtagatg ctttcggtgc catctctaaa ggtgctcaac gctggctgga cctcggtatt
    13261 gttcgttttc agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt
    13321 atcaaccgcg acgtttgccc accgtcgttg aagaacacag gcatcgcgct ggtgctgata
    13381 tttatgccca cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg
    13441 ctttccggtc tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca
    13501 gtagtgctgg tggcggcgtt cattcctatt ctgtggttct tcctgatgca tgattaccag
    13561 cgccagcgcg taatgatgct cctagacccg gaatcagacc cactcggcgc gggctatcac
    13621 attattcagt ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcat
    13681 ggcactcagt cacaacttga atttctaccc gaacgccata ccgactttat cttcgcggta
    13741 ctggcggaag agctgggatt agtgggcatt ttgattctgc tcgctctcta cattctgctg
    13801 atcatgcgcg ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct
    13861 ggcggcttaa tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt
    13921 attctgccgg ttgtaggggt tccgctccca ctggtcagtt atggaggatc agcgctaatt
    13981 gtgctgatgg ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg
    14041 aaaagcgtgt aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg
    14101 aatgctcgcg gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc
    14161 tgcggtatgt aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact
    14221 gaacgcgacg gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga
    14281 tccgtctcga tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa
    14341 cctgacggcc tctggcgaag ctttcgatcc gacacagctg acggcggccc atccaacgct
    14401 tccgatcccc agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg
    14461 cattaatgat cgcggccctt acggcaacga ccgcgttatt tcgctttctc gcgcggcagc
    14521 tgaccgtctt aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca
    14581 ggatggttcg ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc
    14641 cctgcctgca cctcccgatt taagcggtgg cgcgggaaca agttcagtgt ctggcccgca
    14701 gggtgacatt cttccggtca gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc
    14761 ggtaaccagt agcggtttcc tcggcgcacc aacgacctta gcgcctggtg tactggaagg
    14821 cagcgaaccg acgcctgcgc cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac
    14881 ctcgcctgca atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt
    14941 gcaagtcggg gccgttagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca
    15001 gaagttcggc gtccccggtc gcgtaactca aaatggcgcg gtctggcgga tccagcttgg
    15061 ccccttcgcc agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca
    15121 attacagtca tttattacca ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt
    15181 ctgtaaatga cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc
    15241 tatagtaggg cacttttttt aattccatca cggatgtcgt agttcagacc atgaatacca
    15301 ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta
    15361 tctctgctgc acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga
    15421 tcgatgcgga gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga
    15481 acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg
    15541 gccaagcaat gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg
    15601 catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca
    15661 tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt
    15721 gtgtcgccat ggctgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca
    15781 gctacgttaa cgccctgggc ctgaaaaaca cccacttcca gacggtacat ggtctggatg
    15841 ctgatggtca gtacagctcc gcgcgcgata tggcgctgat cggccaggcg ttgatccgtg
    15901 acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc
    15961 agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg
    16021 gacacactga caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct
    16081 tgatctctgc ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac
    16141 tgctgacctg gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt
    16201 tcgcctctga accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag
    16261 acatgtacct gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga
    16321 acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc
    16381 agcttgatgg caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag
    16441 gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt
    16501 aaaaattaaa cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa
    16561 ctcccgcctt ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac
    16621 caaacttaac gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc
    16681 gttacctgag ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta
    16741 caccccaacg gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa
    16801 cgccactcat atcgagcagg ttgaaatact gtatgaagaa ctgggcaaaa tcgatattgt
    16861 ccgcatggtt ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct
    16921 cctcgctgtg atatactttt cccccacttt tactcattct ccacggagat gccgttttgt
    16981 atcaggataa aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccagg
    17041 ctatgcatga attcaccgat acccgcgatg atagtaccct tgatgaaatc tggctggtcg
    17101 agcactatcc ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg
    17161 gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac
    17221 aacaggtgat gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg
    17281 tgaccttgct tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc
    17341 ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac
    17401 gaattcgacg cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac
    17461 catttttacg tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat
    17521 ggaaacccga agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc
    17581 tactaaacaa tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat
    17641 ttacactggg tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat
    17701 attttcttta tcacttgcag aaatgaggtg ccacgccttg ctccgtaact caccccattg
    17761 attaacctat tgaaatagtt gattatggtg agcaaaagcg ttccgttcca attagacata
    17821 agattattgt aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg
    17881 atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa
    17941 tatttacaac cttacgaaat attgctctta accttctgac tatttttgaa gctgtatatg
    18001 tacataaagg gatcgttaat gcagcgaaag tgcttaatct gcccccctcg gcaatcagtc
    18061 agtctattca gaaactgcgc gttatattcc ctgacccatt gtttattcgc aaaggccaag
    18121 gtgtcactcc taccgcattt gcgatgcatc tacatgagta tatcagtcag ggccttgagt
    18181 ccattcttgg cgcgctggat atcgaaggaa gctatgataa gcaacgaacg ataactattg
    18241 cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc
    18301 actatccgca gcttttactg cgcaacccgc ccatcagcga cgcggaaaac caactcagtc
    18361 agtttcaaac cgatctcatc atcgataaca tgttttgcac caatcgtacg gtgcaacatc
    18421 atgttctgtt caccgacaat atggtgttaa tttgccgcga gggaaatcca ctactctctt
    18481 tagaagatga cagagagact atcgacaacg ctgcgcatgt acttctgtta ccggaagagc
    18541 aaaatttcag cggtctgcgg cagagagttc aagaaatgtt tccggaccgg caaatcaatt
    18601 tcaccagcta caacattttg acaatcgctg cactggttgc caacagtgac atgttagcga
    18661 ttattcccag ccgtttttat aacctgttta gccgctgctg gccgctggaa aaactgcctt
    18721 ttccgtcctt aaataaggag caaatagatt tctctatcca ctacaacaaa ttcagcctgc
    18781 gtgatcccat cctgcacgga gtaatagatg ttatccgtaa tgcattttga aaaagtattg
    18841 ttacagccag atcacagcgc agaacgataa aatggcacaa tccaacaaca attttacatt
    18901 ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga tatactgcca gtcgttaatt
    18961 caaaaatagt tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc
    19021 aactggaaca cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc
    19081 gatgccgata agatggccct tatcccggtt aaaaacgtgg caatagagcg cgaagccctg
    19141 ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc
    19201 atcaaagccg caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct
    19261 aacctggcgg aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt
    19321 acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat
    19381 gaaccagtga aactggcgca gaccattgcc gatatggcgc tgcgttatgt ggttatcacc
    19441 tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc actttgcgga ttgcattact
    19501 gccattcggg aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgtggt
    19561 cgtatggatc gtgctctgga tattctgact gcaacgccac cagatgtgtt caaccataac
    19621 ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg gtgcagatta caactggtcg
    19681 ctgaagctgc tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtcta
    19741 atggtgggac tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt
    19801 catggtgtga cgatgttaac gctggggcaa tatttgcagc caagccgcca tcacctgccg
    19861 gttcaacgtt acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg
    19921 ggctttaccc atgctgcatg cggtccgttt gtccgttctt cttaccacgc cgatttgcag
    19981 gcgaaaggga tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa
    20041 cccgcttttt gaagcggttt ttttgtatcg aacaagatat tgagggagcg tcctgctcgc
    20101 cacgtcactc tttatgagag agcttttcag cctgaagatc aacgtctgcg ccttttttcg
    20161 ccgcagcatc atcatcattc atcgccttct tgaacccttt aatggccgct ccaaggtctc
    20221 cgcccagcgt acgtaacttc ttagtcccaa acagcagaac gaccagcgcc gcaactacta
    20281 gcagtttggt aatactaatc tcacccatag ataccttctt gacatataac cggcaacaga
    20341 acgcgcctta aaaaccagag aatattaacg gtcattcgac gcgcgcacac aatagcaatc
    20401 tgtaacaagg tgaatcaagc gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa
    20461 gcgacggttg tttaagacgg gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac
    20521 ttccgccata atgagtgcag gcatttctga cgctgccgca atggtgacgc caaagggatc
    20581 tataatccgg ctttgcccga tatttttgtt cccgcactcg cccgccgcca ccatataaca
    20641 ggtggtatcc agcgcacgag cggcaagcaa cgttgaccag tgatgctctt tgagcggccc
    20701 gcgaacccag gcggcaggaa gtaccaggat ttcagctccc tgtaatgcct gtgccagcgc
    20761 cagctctgga aagcgtaagt cataacaggt catcagaccg accttcatcc cttccacctc
    20821 cagtaacgga gcgatttcat taccagcatc aacacggcgt gattcctgaa tggcaaatgc
    20881 atcatagaga tgcagtttgg cataacgggc gacgatgttt cctgcctgaa gtgccaccag
    20941 catattccat gcgcgccccg gcgttgaagg aacatgaatc gtcagaattg tcgtcatcat
    21001 gttacgttta ctttctcgcc gtaaacgtcc gaggaattcg ccttccagca gctgtgctga
    21061 tttaaccgat agatctgcat catgatcatc gcgtgccagc aatgcttccg gcaggacaaa
    21121 cagcgatacg gcgttttccg ccgcctgcgc catcaacgag gcacaaatct cagcgttctt
    21181 ttcccacaca gatgtaacag caaactgtcc tgcagcaacc agcattgcca cctcctgtca
    21241 gagtcgtaaa attcgccaac gacgattatt cggcgttaca cttatcactc atacaaatca
    21301 aatagcagga ttttgcagtg ttacaacttc ttttagcagt ttttattggc ggtggtacgg
    21361 gaagcgtggc gagatggctg ttaagtatgc gatttaaccc actgcatcag gcgattccgt
    21421 tggggacgct gacagcaaac ctgattgggg cattcatcat aggaatggga ttcgcatggt
    21481 tcagcaggat gacgaacatt gatccagtgt ggaaagtatt aatcaccacc ggattttgtg
    21541 gcggtctaac aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggtc
    21601 gctttggctg ggcattactg aacgttttcg tcaaccttct ggggtctttt gccatgacag
    21661 cactggcttt ctggctgttt tcggcctcaa ccgcacacta aagacccaaa aaaaacccgc
    21721 tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca
    21781 gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt
    21841 ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc
    21901 ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta
    21961 gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgcgtcggt taccagtaca
    22021 ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat
    22081 ttttgatgtt acttatttta gctattgatt ttaaagaagt tactaaaact tcatttgtct
    22141 caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg
    22201 taggtcatct gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg
    22261 agagggatgt aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa
    22321 tgaaaatttt catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt
    22381 tcccatttgt tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt
    22441 tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt
    22501 ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa
    22561 tgttcaggct gttgccaggt tggtgcaata ttttctctaa acgttgtcat ccactcatct
    22621 gcgttagcaa aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg
    22681 acatatttac tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca
    22741 tagcaaagct acaattaaca taaccttaat agacccaaca taaagaataa tctgaatagc
    22801 tgctctattt atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg
    22861 cgtcgttttc tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa
    22921 atgcaatcaa ggaaaaatta ttttattttt taacgagata caacaatcac cttaacaaag
    22981 tatataatat atatcgtcaa cctatgctca tattagatta ttttttgagc gcacagccgc
    23041 agctaacacc attgcaatta acaaatttgc atcaatccac catcaatttg cacacattat
    23101 tatgtgataa ttgccaaccg ctaaatatgc gttttgttat ctatgtataa aaacagtaac
    23161 ttcaatgtct taatggcagt ttttcttgat tttaatcagc attcatcgcc aatttattgg
    23221 gcatattttt tccttaagct ttaggaattt tttattattt actttggggc ctggagacag
    23281 gaaaaattat gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag
    23341 ctcgctacat cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat
    23401 tgctgattat cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag
    23461 gctacagcgc cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg
    23521 acctcggcat gatgattatg atgctgtgtg gatttgccgc ttacatgacc catatcggcg
    23581 cgaatgatat ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc
    23641 tgctgatgat tgccgcctat tttgtcgcct gtctgatgtc tctggccgtc tcttccgcaa
    23701 ccggtctggg tgttttgctg atggcaaccc tatttccggt gatggtaaac gttggtatca
    23761 gtcgtggcgc agctgctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt
    23821 caggggatgt ggtgctggcg gcgcaagctt ccgaaatgtc gctgattgac ttcgccttca
    23881 aaacgacgct gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct
    23941 ggcaacgtta tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa
    24001 tcaccaccac tgctcctgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac
    24061 tgatttttga cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta
    24121 tgctgattgc ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg
    24181 gtctggaagt ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg
    24241 ttgccgctgg ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt
    24301 ctatcgctac ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga
    24361 caatgctggc ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga
    24421 tgatcccgaa actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc
    24481 tgcaggcgtc aaaccttggc cgtacccttt cgcccgtttc tggcgtagtc gttgcggttg
    24541 ccgggatggc gaagatctcg ccgtttgaag tcgtaaaacg cacctcggta ccggtgcttg
    24601 ttggtttggt gattgttatc gttgctacag agctgatggt gccaggaaca gcagcagcgg
    24661 tcacaggcaa gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc
    24721 actgtggtat atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag
    24781 atggcggctg gcgcaatatt caagataacg tctggcagac gtgcggctga tggtcagtgc
    24841 ctgcgccacc gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc
    24901 gtttagcgtc aagggatcaa tgccggtcgg cagttcgtct ttaggttcac cgcgagcata
    24961 ggcattaaac atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg
    25021 tttacgctgg cggaaacggg ttagcgtttg ccccagccgt tcataggcaa tgggcttaat
    25081 gagataatca aatacaccac aacgtacggc ttcagacacc gtttccatat cgctggctgc
    25141 agtggtaaac accacgtcgc cgggataatg cgcctgcacc agttcatgca ataaattaat
    25201 ccctctgccg tcaggaagat agttatcgag caagattagc cccggcttaa aacgctcgat
    25261 catcattcgg gcctgcgcca ggtttcccgc cagtaatatc tgactgaatc cgggaatgtg
    25321 acgaatatat tccgcatgca tctctgccag cggcgtttcg tcctcaacga tcaatagggt
    25381 taatggagct gtcattaggt ttcactttcg gaatatagat tgaaaataag gtaccgcagg
    25441 gatcattatc ttcgagagtg ataacaccac cgcagcgcgt tacgtagctg gcaatcaagt
    25501 acaacccaat gccatgttca ccgggctcgt cagcacgcgt actgaccccc tgctcaaata
    25561 ttttgtctcg tagagactct ggaacgccgc agccctgatc ggcgacttca atcaccacat
    25621 catcgccttc atcgctgagg aataattcaa cgatcttgtt tccttcatcg ctacgcaggc
    25681 tggcttcgaa ggcgttatca agtaaattgc ccgcaatggc tgcaaactcg gtgctatcca
    25741 gtcctggcgg cagttgcgaa agctggctac cggggacaat gatcattttt agccccagtt
    25801 cccgggcgcg ctgcacttta ccaaaaagca gccccgccac ctagcgatcg gcaaacgcct
    25861 cgcgcaggct gtcaataagc tgttgctggg cctgagactc cccctgcacc atcgccagca
    25921 cgcgatcata ctctttcatc tgcaacagac cattgagcgt cgacatccaa ttgagatgct
    25981 cgtgtcgcaa tgtacgaagg ctctcaacgt attgttttat ttgcgtcagt tgcgcattga
    26041 gggtggaaat ttcgtcttta ctgcgaaagc tgataatggc ccccagcaaa tcatcacctg
    26101 aacgaatagc ttcccgattg gcaataacgc tcagaccgtt aaagttagcc accatatcct
    26161 gacgtttttc atcaatctgt tcggtaaaga aatcggcggg cctgaccact tcagcaatgg
    26221 gtttacccaa ccattgccgt ccgggggaac tcagccccag catctttctt gcgttacgat
    26281 tgatggcggt aatgtaaccg tgcggatcca ccgcaatcag cccttcataa accgaactaa
    26341 acagcgcctc ttgctgacgg accacgcgtg cgatttgctt tggctccatg cccatcatct
    26401 gccgacggat atgcgcggcc aggaaccacg acagcaacat cagaatccct aacagcacga
    26461 caaacacacc tgccatcggt aataaaaact cagcccgcca gctatcgatt ttactcacca
    26521 ggtagccaat cgacaccacg ccgatgactt ttccatcgtc atcaaagatt ggcgttttgg
    26581 cgcgcatcgc catccccatt gaccctttcc cggtaatgaa gtagctctcc cctttctcca
    26641 gcgcgccctg tttggtgaac tgcataggat aaccaatttt ctccggatta ggatggtaaa
    26701 ggcggatcga gtgccggtcg ccatcaccac ataatcaaaa tcggtatctc tttgtaattt
    26761 gttagcgatg gtcgccagcc gtttgtagtc acgcgttttc accgcagaga tgacactgtc
    26821 attggaggca ataattttcg cctgattcat tgccatgtcg cgtacatgaa gcgttaaata
    26881 gtcctcaaaa ctggccgtaa aatattgcgc caaggctgca atgacaaata ttgagaacac
    26941 cagaatcagc aaaaagatac gcagcggaaa tgccagtctt tggaaaaatg caaactgttt
    27001 attctcgtta agctgcaaca ttgttttcct tgcgggttat cccgagcctc tttgacagat
    27061 gtaattaaaa tgttgaatca aaatacatag atattaatta tgatcgtggc aaattttatt
    27121 cattaaatca attaaatcac ttaaacctgc aggtcatttc gtaattacat taattaaaaa
    27181 acttaaacca ttaaataaat aaaaatttca cgccgaaata ctccttagga tgtatagcga
    27241 aaggagaaaa agatatacct cgatcacccc cctttctccc aagtgaaaat aaaaggttat
    27301 cagtttgcaa cattgaacaa cattcgttgc aaatcgataa caacatgcac cttcaggata
    27361 ctatttatta tgttcggcaa tgatattttc acccgcgtaa aacgttcaga aaataaaaaa
    27421 atggcggaaa tcgcccaatt cctgcatgaa aatgatttga gcgttgacac cacagtcgaa
    27481 gtatttatta ccgtaacccg cgatgaaaag cttatcgcgt gcggtggaat tgccggaaat
    27541 attattaaat gcgttgctat cagtgaatcc gttcgcggtg aaggactggc gctgacatta
    27601 gccactgaac tgataaacct cgcctatgag cggcacagca cgcatctgtt tatttatacc
    27661 aaaaccgaat acgaggcgct gttccgccag tgcggttttt ccacgctgac cagcgtaccc
    27721 ggcgtgatgg tgctgatgga aaacagcgcc acgcgactga aacgctatgc cgaatcgctg
    27781 aaaaaatttc gtcatccagg gaacaagatt ggctgcattg tgatgaacgc caatcccttt
    27841 acgaatggtc accgttatct gattcaacaa gctgcggcac agtgcgactg gttgcatctg
    27901 tttttagtca aagaagattc ttcacgcttc ccctatgaag accggctgga tctggtgtta
    27961 aaaggcaccg ccgatattcc acgcctgact gtgcatcgcg gctccgaata catcatctcc
    28021 cgcgctacgt tcccttgcta cttcattaaa gaacagagcg tcattaacca ttgttacacc
    28081 gaaattgatc tgaagatttt ccgtcagtac ctcgctcccg cactgggtgt aactcaccgc
    28141 tttgtcggta ctgaaccctt ttgtcgcgtt accgcccagt acaaccagga tatgcgctac
    28201 tggctgaaaa cgccgactat ctccgcaccg cccatcgaac tggttgaaat tgagcggctg
    28261 cgttaccagg agatgccgat atccgcttcc cgggtacgtc aactgctggc gaaaaacgat
    28321 ctcacggcta tcgcgccgct ggtccctgca gtcacgctgc attatttgca gaacctgctt
    28381 gagcactccc gccaggacgc ggcagctcgt caaaagaccc ccgcatgaga aacaggtgaa
    28441 aaatgaaaat aaaccagccc gccgttgcag gcacccttga gtctggggat gtgatgatac
    28501 gcatcgcccc actcgatacg caggatatcg acctgcaaat caacagcagc gttgagaaac
    28561 agtttggcga tgcaattcgc accaccattc tggacgttct cgcccgctac aacgtgcgcg
    28621 gcgtacagct gaatgtcgat gacaaaggca cactggactg cattttacgt gcacgactgg
    28681 aagccctgct ggcacgcgcc agcggtatcc cgactctgcc atgggaggat tgccaatgat
    28741 ttccgcttcg ctgcaacaac gtaaaactcg cacccgccgc agcatgttgt ttgtgcctgg
    28801 tgccaatgcc gcgatggtca gcaactcctt catctacccg gctgatgccc tgatgtttga
    28861 cctcgaagac tccgtggcat tacgtgaaaa agacaccgcc cgccgcatgg tttatcacgc
    28921 gctgcaacat ccgctgtatc gcgatattga aaccattgtg cgtgtcaacg cgctggattc
    28981 cgaatggggt gttaacgacc tggaagccgt cgttcgcggt ggtgcggacg ttgtgcgtct
    29041 gccgaaaacc gataccgctc aggatgttct ggatattgaa aaagagatcc tgcgtatcga
    29101 aaaagcctgt ggtcgtgaac ccggcagcac cggcctgctg gcggcgattg aatctccgct
    29161 gggcattacc cgcgcagtgg aaatcgctca cgcttccgag cgtttgatcg gtatcgccct
    29221 cggtgcagaa gactatgtgc gcaacctgcg tacagaacgc tccccggaag gaactgaact
    29281 gctgttcgca cgctgttcca ttttgcaggc cgcgcgctct gcgggtattc aggcgttcga
    29341 taccgtctat tccgacgcta acaacgaagc cggattcctg caagaagccg cccacatcaa
    29401 acagctgggc tttgacggca aatcgctgat caacccgcgt cagattgatc tgctgcacaa
    29461 cctctacgca ccgacccaga aagaagtgga tcacgcccgc cgcgtcgtag aagccgctga
    29521 agccgccgct cgcgaaggcc tcggcgtggt ttccctgaac ggcaagatgg tggacggtcc
    29581 ggttatcgat cgcgcccgtc tggtgctctc ccgtgcagaa ctttccggca tccgcgaaga
    29641 ataaggcaat caaaatgacg cagaaaattg aacaatctca acggcaagaa cgggtagcgg
    29701 cctggaatcg tcgcgctgaa tgcgatcttg ccgctttcca gaactcacca aagcaaacct
    29761 accaggctga aaaagcgcgc gatcgcaaac tgtgtgccaa cctggaagaa gcgattcatc
    29821 gctctggttt acaggacggc atgacggttt ccttccatca cgctttccgt ggcggtgacc
    29881 tgaccgtcaa tatggtgatg gacgtcatcg cgaagatggg ctttaaaaac ctgactctgg
    29941 cctccagctc cctgagtgat tgccatgcgc cgctggtaga acacattcgc cagggcgtgg
    30001 ttacccgcat ttatacctct ggcctgcgcg gtccactggc ggaagaaatc tcccgtggtc
    30061 tgctggcgga accggtgcaa atccactctc acggcggacg tgtgcatctg gtacagagcg
    30121 gcgaactgaa tatcgacgtg gctttcctcg gcgtcccatc ctgtgatgaa ttcggtaatg
    30181 ccaacggtta caccggtaaa gcctgctgtg gctccctcgg ctatgcaatg gttgatgccg
    30241 acaacgcaaa acaggtcgtg atgcttaccg aagaactgct gccttatccg cataatccgg
    30301 caagcattga gcaagagcag gttgatttga tcgtcaaagt tgatcgcgtt ggcgatgctg
    30361 caaaaatcgg cgctggtgcg acccgtatga ccactaaccc gcgcgaactg cttattgccc
    30421 gtagcgctgc ggatgtgatt gtcaactctg gctacttcaa agaaggtttc tccatgcaaa
    30481 ccggcaccgg cggcgcttcg ctggcggtaa cccgtttcct ggaagacaaa atgcgtagcc
    30541 gcgatattcg cgccgacttc gccctcggcg gtattactgc aacgatggtt gatctgcacg
    30601 aaaaaggtct gatccgcaaa ctgctggatg tgcagagctt tgacagccat gctgcgcaat
    30661 cgctggcccg taaccccaat cacatcgaaa tcagcgccaa ccagtacgct aactggggtt
    30721 cgaaaggcgc atcggttgat cgtctcgacg tggtggtact gagcgcgctg gaaattgaca
    30781 cccagttcaa cgttaacgtg ctgaccggct ctgacggcgt actgcgtggt gcttccggtg
    30841 gtcactgcga taccgcgatt gcctctgcgc tttccatcat cgtcgcgccg ctggtacgcg
    30901 gtcgtattcc gactctggtg gataacgtgc tgacctgcat caccccgggc tccagtgtcg
    30961 atattctggt cacagaccac ggtatcgcag ttaacccgac acgtccggag ctggcagaac
    31021 gtctgcagga agcgggcatt aaagtggttt ccattgagtg gctgcgtgaa cgtgcgcgtc
    31081 tgctgaccgg tgaaccacag ccgattgaat tcaccgaccg cgtcgttgcc gttgtgcgtt
    31141 accgcgatgg ctcggtgatc gatgttgtac atcaggtgaa ggaataagcc atgcacctgc
    31201 ttcctgaact cgccagccac catgcggtgt caattcccga gctgctcgtc agccgggatg
    31261 aaaggcaagc acggcaacac gtctggctca agcgccatcc tgttccactg gtctccttta
    31321 ccgtggttgc gcctaggccg attaaagaca gtgaggtcac acgccgaatt tttaatcatg
    31381 gcgtgacagc cttgcgtgcc ttagccgcaa aacagggctg gcaaattcag gagcaggctg
    31441 cactggtttc cgccagcggg ccggagggta tgttgagcat tgccgccccg gctcgcgacc
    31501 tcaagctcgc caccattgag cttgaacata gtcatcctct cgggcggtta tgggatatcg
    31561 atgtcctgac gcccgaaggc gaaattctct cccgccgcga ctattcactg ccgcctcgcc
    31621 gctgcctgtt gtgcgagcaa agcgcagccg tctgcgcgcg tggaaaaccc atcaactgac
    31681 cgatttactc aaccgcatgg aggcactgct gaacgatgtc gatgcctgca acgtcaacta
    31741 aaaccacaaa gcttgcgacg tcattaatcg atgagtacgc cctgctgggc tggcacgcca
    31801 tgctgactga agtcaatctg tcaccgaaac caggtctcgt ggatcgcatt aactgcggtg
    31861 cgcataaaga tatggcgctg gaagatttcc accgcagcgc gctggcgatt cagggctggc
    31921 taccccgttt cattgaattt ggtgcctgta gtgcggaaat ggcaccagaa gcggtactcc
    31981 acggattacg cccaattggt atggcttgcg aaggtgatat gttccgcgcc accgcgggcg
    32041 taaacacgca taagggcagc attttttctt tagggctact gtgtgcggca attggccgtt
    32101 tgcttcagct caaccagtcg ctaacgccaa caaccgtttg ttctacggcg gcaagtttct
    32161 gccgtggcct gaccgatcgc gaactgcgta ccaataattc acaactgacg gcaggtcaac
    32221 ggttgtacca gcagcttgcc cttaccggcg cacgcggtga agccgaagcg ggttatccac
    32281 tggtgatcaa ccacgccttg ccgcattacc tcactctgct ggatcagggg ttagatcctg
    32341 aactggcatt gctcgatacc ttgctcctac tgatggcgat caacggcgat accaacgttg
    32401 cttcgcgcgg tggcgagggg gcctgcgctg gctacagcgc gaggcgcaaa cattattgca
    32461 aaaagggggc attcgaaccc ccgccgatct cgattatctc cggcagttcg acagggagtg
    32521 tatcgaacga aatctcagtc taggcggcag tgccgacctg ctgatcctta cctggttttt
    32581 agcacagatt taattattta agcacttgat aaatttggaa atattaattt tcggagaacc
    32641 cgtatgtctt tagcaaaaga taatatatgg aaactattgg ccccactggt ggtgatgggt
    32701 gtcatgtttc ttatccctgt ccccaacggt atgccaccac aggcatggca ttacttcgct
    32761 gtgtttgtgg caatgattgt cggcatgatc ctcgagccaa ttccggcaac agcgatcagt
    32821 tttattgcgg ttactatttg cgttattggc agtaattacc tgctctttga tgccaaagaa
    32881 ttagctgacc cagcgtttaa tgcgcaaaaa caggcgctga aatggggtct ggctggtttt
    32941 tccagcacca cggtatggct ggtatttggc gcatttattt ttgcattagg gtatgaagtt
    33001 tccgggttag gtcgtcgcat tgcccttttc ctggtgaaat tcatgggcaa acgcacgctg
    33061 acgttgggtt atgcgattgt cattatcgac attctgctgg cgccgtttac accgtccaac
    33121 accgcgcgta ccgggggtac ggtttttccg gtcattaaaa acctgccgcc gttgtttaaa
    33181 tcattcccca acgatccgtc cgcgcgtcgt attggcggct atttgatgtg gatgatggtc
    33241 attagtacca gtctgagttc gtccatgttt gtcaccggtg cggcaccaaa cgtgctgggt
    33301 ctggagttcg tcagcaaaat tgccggtatc cagattagct ggttgcagtg gttcctctgc
    33361 ttcctgccgg ttggggttat cttgcttatc attgcgccgt ggctttccta cgtgctgtac
    33421 aaaccggaaa tcacacacag tgaagaagtg gcaacctggg cgggtgatga actgaaaacc
    33481 atgggtgcgc tgacacgcag agagtggaca ctgattggcc ttgtattgct cagcttaggt
    33541 ttgtgggttt ttggcagtga agtcattaat gctactgcag ttggtctgct ggcagtttcg
    33601 ctaatgctgg ccctgcacgt tgtgccttgg aaagatatta cccgctataa cagcgcatgg
    33661 aacacgctgg tcaacctggc aactctggtt gtgatggcta acggcctgac tcgttctggc
    33721 tttattgact ggttcgccgg taccatgagt acgcacctgg aaggattctc accaaacgca
    33781 acggtgattg tactggttct ggtgttctac tttgcacact acctgtttgc cagcctgtct
    33841 gcgcacaccg caaccatgct gccggttatt ctggccgtcg gtaaaggtat tccgggcgta
    33901 ccaatggaac aactgtgtat cctgctggtg ctgtctatcg gtatcatggg ctgtctgacg
    33961 ccctatgcaa ccggtcctgg ggtgattatt tacggctgtg gctatgtgaa atcaaaagat
    34021 tactggcgtc ttggcgcaat cttcggggtg atttatatct ctatgttgct gttggttggc
    34081 tggccgattc tcgccatgtg gaactaatac gttcgccaaa cgctgaataa atcatcacgc
    34141 ccgccaggta agcccacctg gcgggcgtga tgatttaata gatagtattc agtgtatgta
    34201 tgagttccac acccattatg aaagcattct ggcgtaacgc cgcgttgctc gcggtttctc
    34261 tgcttccctt ctcttctgcc aacgccgtag cgttgcaggc aaaacagtat ggcgattttg
    34321 atcgctatgt cctggccctc tcctggcaaa ccggattttg ccagagtcaa cacgatcgaa
    34381 atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac gaccaacaaa gctgattttc
    34441 tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt tgctgcccgt ggtgttgatg
    34501 aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat cccgaatcta ccagaagcgc
    34561 acgccagccg gatgtgttca tcgccggaaa ccgggttatc actggaaacg gccgctaaac
    34621 taagtgaagt catgccagga gctggcggac gttcctgcct ggaacgctac gaatatgcca
    34681 aacacggtgc ctgctttggt tttgatccgg acgcatactt cggtacgatg gtacgcctga
    34741 atcaagaaat taaagagagc gaagccggaa aattccttgc ggataattac ggtaaaacag
    34801 tgagccgccg tgactttgac gccgcctttg ccaaaagctg gggaaaagag aacgtgaaag
    34861 cagttaagct aacgtgccag ggtaaccctg cgt
//
LOCUS       DAJTSL010000026        34851 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTSL010000026 DAJTSL010000000
VERSION     DAJTSL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.026"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..761)
                     /gene="istA"
                     /locus_tag="OO433_001107"
                     /pseudo
     CDS             complement(1..761)
                     /gene="istA"
                     /locus_tag="OO433_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1377..1514
                     /locus_tag="OO433_001108"
     CDS             1377..1514
                     /locus_tag="OO433_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049657.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            2114..2893
                     /gene="ipaJ"
                     /locus_tag="OO433_001109"
     CDS             2114..2893
                     /gene="ipaJ"
                     /locus_tag="OO433_001109"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6049658.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3837..4766)
                     /gene="virB"
                     /locus_tag="OO433_001110"
     CDS             complement(3837..4766)
                     /gene="virB"
                     /locus_tag="OO433_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6049659.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4983..5219)
                     /locus_tag="OO433_001111"
     CDS             complement(4983..5219)
                     /locus_tag="OO433_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6049660.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(5223..7124)
                     /gene="ipaA"
                     /locus_tag="OO433_001112"
     CDS             complement(5223..7124)
                     /gene="ipaA"
                     /locus_tag="OO433_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6049661.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNDLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDAVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSTNNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(7133..8131)
                     /gene="ipaD"
                     /locus_tag="OO433_001113"
     CDS             complement(7133..8131)
                     /gene="ipaD"
                     /locus_tag="OO433_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6049662.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKDLSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSKK
                     EYPINKDARELLHSAPKEAELDGYEMISHRELWDKIAKSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVKSLKDELTKLKEKYKDKPLYPANNTV
                     SKEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(8182..9273)
                     /gene="ipaC"
                     /locus_tag="OO433_001114"
     CDS             complement(8182..9273)
                     /gene="ipaC"
                     /locus_tag="OO433_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6049663.1"
                     /translation="MEIQNTKSAPILYTDISTKQTQSSSETQKSQNYQQLAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTITPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETRLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISEQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRAYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYTSALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDNINQSRNSTASQIAGNIRA"
     gene            complement(9293..11035)
                     /gene="ipaB"
                     /locus_tag="OO433_001115"
     CDS             complement(9293..11035)
                     /gene="ipaB"
                     /locus_tag="OO433_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6049664.1"
                     /translation="MHNVSTTTTGLPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LAANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPENKKLRREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGN
                     VASAVFQNNASKNLADLTLSKYQVEQLSKYIGDAIEKFGQLQEVIAELLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(11041..11508)
                     /gene="ipgC"
                     /locus_tag="OO433_001116"
     CDS             complement(11041..11508)
                     /gene="ipgC"
                     /locus_tag="OO433_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6049665.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(11566..12192)
                     /gene="ipgB1"
                     /locus_tag="OO433_001117"
     CDS             complement(11566..12192)
                     /gene="ipgB1"
                     /locus_tag="OO433_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6049666.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(12208..12597)
                     /gene="ipgA"
                     /locus_tag="OO433_001118"
     CDS             complement(12208..12597)
                     /gene="ipgA"
                     /locus_tag="OO433_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6049667.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(12610..14094)
                     /gene="icsB"
                     /locus_tag="OO433_001119"
     CDS             complement(12610..14094)
                     /gene="icsB"
                     /locus_tag="OO433_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6049668.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAESFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEV
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            14408..16024
                     /locus_tag="OO433_001120"
     CDS             14408..16024
                     /locus_tag="OO433_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6049669.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNQKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTNYLNNKNWEPINKNINY
                     NGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESKHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRQELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKELNSKRGEPTKLLIRNSDGLLQEVKVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEILNKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            16034..16396
                     /gene="ipgE"
                     /locus_tag="OO433_001121"
     CDS             16034..16396
                     /gene="ipgE"
                     /locus_tag="OO433_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6049670.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            16396..16854
                     /gene="ipgF"
                     /locus_tag="OO433_001122"
     CDS             16396..16854
                     /gene="ipgF"
                     /locus_tag="OO433_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6049671.1"
                     /translation="MSRFVFIFLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16863..17978
                     /gene="mxiG"
                     /locus_tag="OO433_001123"
     CDS             16863..17978
                     /gene="mxiG"
                     /locus_tag="OO433_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6049672.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKSGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICETNKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17986..18237
                     /locus_tag="OO433_001124"
     CDS             17986..18237
                     /locus_tag="OO433_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscF/YscF/HrpA family type III secretion system
                     needle major subunit"
                     /protein_id="HCR6049673.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGQLTSALNALAENPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            18250..18543
                     /gene="mxiI"
                     /locus_tag="OO433_001125"
     CDS             18250..18543
                     /gene="mxiI"
                     /locus_tag="OO433_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6049674.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSHPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            18549..19274
                     /gene="mxiJ"
                     /locus_tag="OO433_001126"
     CDS             18549..19274
                     /gene="mxiJ"
                     /locus_tag="OO433_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6049675.1"
                     /translation="MIRYKCFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            19271..19798
                     /locus_tag="OO433_001127"
     CDS             19271..19798
                     /locus_tag="OO433_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6049676.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIEWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19740..20435
                     /gene="mxiN"
                     /locus_tag="OO433_001128"
     CDS             19740..20435
                     /gene="mxiN"
                     /locus_tag="OO433_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6049677.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            20428..20835
                     /gene="mxiM"
                     /locus_tag="OO433_001129"
     CDS             20428..20835
                     /gene="mxiM"
                     /locus_tag="OO433_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6049678.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDENIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20819..21247
                     /gene="mxiM"
                     /locus_tag="OO433_001130"
     CDS             20819..21247
                     /gene="mxiM"
                     /locus_tag="OO433_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6049679.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            21377..22009
                     /gene="mxiE"
                     /locus_tag="OO433_001131"
     CDS             21377..22009
                     /gene="mxiE"
                     /locus_tag="OO433_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6049680.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21996..23696
                     /gene="mxiD"
                     /locus_tag="OO433_001132"
     CDS             21996..23696
                     /gene="mxiD"
                     /locus_tag="OO433_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6049681.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKMTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSQGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTRNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23715..24782
                     /gene="mxiC"
                     /locus_tag="OO433_001133"
     CDS             23715..24782
                     /gene="mxiC"
                     /locus_tag="OO433_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6049682.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24795..26855
                     /gene="mxiA"
                     /locus_tag="OO433_001134"
                     /gene_synonym="virH"
     CDS             24795..26855
                     /gene="mxiA"
                     /locus_tag="OO433_001134"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6049683.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26868..27269
                     /gene="spa15"
                     /locus_tag="OO433_001135"
                     /gene_synonym="spaK"
     CDS             26868..27269
                     /gene="spa15"
                     /locus_tag="OO433_001135"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6049684.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            27273..28565
                     /gene="spaL"
                     /locus_tag="OO433_001136"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             27273..28565
                     /gene="spaL"
                     /locus_tag="OO433_001136"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6049685.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMATAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELLGETIR"
     gene            28658..28996
                     /gene="spa13"
                     /locus_tag="OO433_001137"
                     /gene_synonym="spaM"
     CDS             28658..28996
                     /gene="spa13"
                     /locus_tag="OO433_001137"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6049686.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28983..29861
                     /gene="spa32"
                     /locus_tag="OO433_001138"
     CDS             28983..29861
                     /gene="spa32"
                     /locus_tag="OO433_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6049687.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29855..30736
                     /gene="spa33"
                     /locus_tag="OO433_001139"
                     /gene_synonym="spaO"
     CDS             29855..30736
                     /gene="spa33"
                     /locus_tag="OO433_001139"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6049688.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30741..31391
                     /gene="spa24"
                     /locus_tag="OO433_001140"
                     /gene_synonym="spaP"
     CDS             30741..31391
                     /gene="spa24"
                     /locus_tag="OO433_001140"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6049689.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            31406..31666
                     /gene="spaQ"
                     /locus_tag="OO433_001141"
     CDS             31406..31666
                     /gene="spaQ"
                     /locus_tag="OO433_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6049690.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            31666..32436
                     /gene="spa29"
                     /locus_tag="OO433_001142"
                     /gene_synonym="spaR"
     CDS             31666..32436
                     /gene="spa29"
                     /locus_tag="OO433_001142"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6049691.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            32443..33471
                     /gene="spa40"
                     /locus_tag="OO433_001143"
                     /gene_synonym="spaS"
     CDS             32443..33471
                     /gene="spa40"
                     /locus_tag="OO433_001143"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6049692.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            33484..33726
                     /locus_tag="OO433_001144"
     CDS             33484..33726
                     /locus_tag="OO433_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049693.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(34333..34851)
                     /locus_tag="OO433_001145"
                     /pseudo
     CDS             complement(34333..34851)
                     /locus_tag="OO433_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        10781 a         5217 c         7038 g        11815 t
ORIGIN      
        1 cagtgctccc catcgggcgc aggcgagtca ttagtgggat gtaaaaactg ttccatccgt
       61 tccaccttac ctttagtctg tgccctgaag gggcgacaca gtcggggaga gaagcccatc
      121 tccttgccga actgccacag cgaaggatgg aaccggtgct gaccggtctg atatgcgtca
      181 cgttgcagaa ccacagtttt catattgtca tacaacactt cgcgcggcac accaccaaag
      241 aagcggaacg cattacgatg gcaggtctcc agcgtgtcat aacgcatatt gtcagtgaat
      301 tcgatgtaca acattcggct gtatccggga acagcaacga acacgtgaag cggtgagcga
      361 ccattacgca tagtgcccca gtcaacctgc atctgtcgtc cgggttcagt ttcgaaccga
      421 acggcaggct cctgctcctg aggaaccgag agagaacgaa tgaatgccct gagaatggtc
      481 attccgccac gatatccctg gtctctgatc tcgcgagcga ttaccgttgc cgggattttg
      541 taaggatgag catcggcgat gcgttgacga atataatccc ggtattcatc caggagtgaa
      601 gcaacagcag gtcgcggcgt atattttggc ggctcagatt ttgcctgcaa ataacgttta
      661 acggtattgc gggagagccc cagttctctg gcaatcgccc ggctactcat tccctgcttg
      721 tgcaggattt taatttccat aactgtctca aaagtgacca taagctctcc tgaatcagga
      781 gagcagatta ccccctggat ctgatttcag gcgttgggtg tggatcacta ttgcaccgtt
      841 cgtgacactt gttgactgcc tgtgcggcat ctggtatcaa ggtggcgtga agccggttca
      901 ggttctcatg cggaacgttg gaacctatat ccccccgata ccaaaggcga aatgtaagtg
      961 acaaaatgca atgaacacgt tctccgttaa ctgaggagca tggtgataaa taaaggtaaa
     1021 tgcagaaggt gtagagtctc gcagatttct gacatcatag agcctaacat tagatgcata
     1081 acgctacaga ttgtagaatc tctacaggtg aaatatacac tggttggcgt ttatgtcgca
     1141 aaacatgtca ttcaggttca tctcaccaat aaggatatga gtgaagtgga ggataagtga
     1201 gtctgctgtc agagttttct ggtgtatgtc agtaactgcg aaccatgtct gcttgatatg
     1261 ggagcttatg gaggctctct gtacaaggtt agggagctaa caaagcaggg ataaaaagtc
     1321 cgcctgatgc aggcacggtt cgttaaatct tttgccattg gtaacaaaat ggtgcgatga
     1381 atgttcaggc tatctttatc ttgatggtgg ttcagctgtt tggtaaagaa atcgctgtaa
     1441 caatagaaga actgcaggca gtactggtcc cacatcgttt gctcagtcaa ctggtgaagt
     1501 tccgtagcgc gtaaattaat gctttgcatg aaacgttact ggagtggatg aaagaatacc
     1561 tgcatatatc attactgcgt tggattacca gtacaacaga ttgaatgaac tggacatgtt
     1621 gatagacggc attgaaaagc agcttataag catagtgcgg cagagttaat tggagagcac
     1681 ttcgccttct gagtattaag taatcatggt ggtacctgcg gatactatct attcactgtg
     1741 ctcggtcggc agccttactg accaaggagt tgggggagtt gataacaaag ttgaaaaaac
     1801 agctctcaac caaggtggtt attgtaacaa tagcaaataa gctggtgtga cctgtatcac
     1861 aataaccgat tatggacgta aatacgacaa aaatatgtca gtatcaggcg gtattaattg
     1921 ctgatgctga tggacattaa ctcttaataa aagggtggat gctgaaaggt tgttatggtg
     1981 acggacgtga tgacgacgga aaagagtatg aggttttgga tttaaagttc gttcggggaa
     2041 taaggacccg gtcagctaat tctcatagta gttcgcattg ctggatgcaa tactgtgctg
     2101 caattactcg tcgatgtcgg aacaacggaa gccttgcaaa cggggatgta ttcatacagg
     2161 tgttatgtta tatggggttt tattgcaggg ggctatacca agagaatata tgatatctca
     2221 tcagactgat gtgagggtaa atgagaatag agtaaacgaa caaggttgtt ttttggcgag
     2281 gaagcagatg tatgacaatt cttgtggagc tgccagttta ctctgtgctg caaaggaatt
     2341 aggggttgat aaaataccac aatacaaagg gtctatgtcc gaaatgacaa ggaagagttc
     2401 tcttgatttg gataatcgtt gtgagcggga tttatactta ataactagtg gtaattataa
     2461 tccgagaata cacaaagata atatcgcgga tgccggttat tctatgcctg ataagatagt
     2521 catggctacg aggcttcttg gattgaatgc ttatgtcgtt gaagaaagta acattttttc
     2581 gcaagtaatt agttttattt atcccgatgc aagggactta ttaattggca tgggatgtaa
     2641 tatagtacat caacgagatg tgttatcttc taaccaaaga gttttggaag ctgttgctgt
     2701 atcctttatt ggagttcctg tcggcctcca ctgggtgttg tgtagacctg atggaagcta
     2761 tatggatcca gctgtaggag aaaattattc ttgtttttca actatggaat taggtgcaag
     2821 aagaagtaat tctaacttta taggatatac taaaataggt atttccatag ttataactaa
     2881 tgaggctttg taaattgtcg ttttttatct gtgaaggagc ttgaatattt tggggcgggg
     2941 gcataacaaa tgatattact gagtacaaat ttagctatat tgggcggatt agttgtgtgg
     3001 gaagacgaaa tgtttctctt gaaataagtg aactccctca aggaatgatt gtgtgcttaa
     3061 atatttaagt gctggtaagt ttgagaaggc tgacatattt tgcagagcaa acagtgattg
     3121 acttggatga tatttttatt ttagtcactc gttgtaattg gagtatgctt tattttattt
     3181 gaaaatagat gtattttata cccgcaattt aaactgtaag ccctatgagg ggtatggata
     3241 ttataagtta ctctttgact aagcataact tttggggtta gaaaaataaa tctcttgtta
     3301 ataattaata tgattttaaa atcaaacttg tttataatta taaaatcatg taagtcggtc
     3361 attttatggg aagattagga gaataaaaca gtaattatat ttgagaaatc tgctaatata
     3421 ttcttaaagc ttttttgata ctgtgaacga agtctgttaa tggtggattt tttgtttatc
     3481 accatgtaaa aaattgagta gtaaaagcgt tgactgctaa tcctcgctgt ttttattgaa
     3541 acgattaaaa atacgctgaa tattttcggc cagtcactca tcttcacata aaattttgac
     3601 catcatgccg catcccttag ttgcacaatt cattttcaat gatatgaata ttactaattt
     3661 caccagataa tgttctcgtg gtcagcaggg tatgagtttt caccgtaatt ttaaggaaac
     3721 acagtgcgtg gggattgaat aacagatagc cgacggaatt accccaccgg ctgaaagtca
     3781 agttcaacga agatttttat tatctgaatt gggcagttta catcagtgtt cgatgtttat
     3841 gaagacgata gatggcgaga aattatatct cgaatagctt catctatttc tctctgaact
     3901 gttttgttta ttttagataa tgtaaatatt agagttctgt ttgttttatt ctcttttctt
     3961 tttatgtaag tccgtttgtc tttagaaata aataacgagt caacttttag cgaagggttt
     4021 tgctttctgt ttttgctttt ctttatgatg tttaaaattt cttttttata tatgtccggg
     4081 ggcaaatttg tatcaaggtc ttttatttct tcctttaata ttggtagtgt agaactaaga
     4141 gattcattag ccttttcaag tcctttataa taattgaata atatcttgta gtcattaaag
     4201 ttaagttctg acgcgattgg aaatagagat attatttcct gtggaacgct tgctgcctga
     4261 aaggcacgag tgactttcgc gcgagacaga ttctcttttt tggctatgtc tttataggac
     4321 atccctgata ctttcagaaa attaagacca ataccaagtt ctcggatgct atgctctttt
     4381 gctgtttgta tatcgtttgc tagttttctg gcatcaagag tagatatata ttcttttgaa
     4441 tatagaactt caagatctgc tcctgcatat attgcagatg ctcttctacg agtgccatcc
     4501 agaatttcaa ttctaccatc aatctccctt cctattacag ggaagaattg ttgtagcttt
     4561 atagttttta tgatatcggc taatgattct tctgttaggg attcttgatc gcgtacattc
     4621 gttttttggt tgacgaaggt taaatcttgg atccttttat gaggaatctt ggctttgata
     4681 aaggaaactt tattaccaga atggagtgta aactctttct tttggccttc ctttatactt
     4741 aacaagtcgt tgcacaaatc caccatatca caccctgttt attcatattg atttatattg
     4801 acaaaagtta aatgcagtgt gagcaagatt gtatgtaact gttgatgata gtcaacggta
     4861 gtcttaaaaa ttactatttt aatagctgga ttgatgcaat ttatctttat tggataaaag
     4921 aataatattc ttgtggagct ctgatgtgag agctccatag atgaaaaaat gcttatcaat
     4981 tactatattg attttgtact atttttgact acagaaataa gatctccaat agtaataatg
     5041 tgttctaatg cagattcatc tatacgtaga ttgaactcaa aactaatcat atccagcatt
     5101 tcaatcagag aaagagaatc ggcagaaaga tcttcaataa atttagtttc ttcagatagt
     5161 ttgctgtacg ctataccata gcaaaaggcc actattgata atattttttc ttttatcata
     5221 ttttaatcct tattgatatt ctttaatact tttgataggg aagtggttac atcttttgct
     5281 gctttatata tattattgtt ttcagctgta atatcattgt tattttttaa atcagatata
     5341 tcatctgtaa gtaattctgc agaggtatcg tcaatttttg acaatacctt gctgagggcg
     5401 ctagatactt cttttgcctt ttcatagatc gcatgatttt tatcaattgt atctgcgttg
     5461 gtttctgtta cgtagtttat tgtatttgca tctgtaactt ttaatttgtt gtttattctg
     5521 tgttctgttg tggattttgt aatgtccttt gatttttcct gtaactctcg ttcttgtgtt
     5581 cctgatttag ttccaatgag agatataaga ttacttaggg agtttgttac tttttttgaa
     5641 gcttcaaata tcgtttcccg tgttaaatat gtgtctccat ttcttgataa aaaatcagtc
     5701 tgaagtactt ttgaatcagt gtttgagttg aatttattga tttgcgaagc tactctatga
     5761 gaaagagaag cgctactttt agttgtatct gatgagttat cattactgct tgtcactcct
     5821 tccgggtgga gagggggggt ggttgaagag ttctgtatgt tcagaggcct gtgtccccga
     5881 gaaagagagt tatttgtaga tctttcttga ctactcgtag ttaacaggtc tgaacctatt
     5941 ccatccgaat tatctggagt atggttgaaa accttttcta aagctgaatt atttgcaaat
     6001 gtctggacag catctgatat agagtgtggt agaacggaag ttactttttg aactagtgct
     6061 ctttgtggat ttagtaatga gtttccgtta cgggataata gatcgtttgt ctgagaagta
     6121 ggagactgtg catcattttc tgggttctcg tgatagctat tgtcatatac tctgttgtca
     6181 aaaactctat tatcgtaagt tctattatca ttatttatat gatagtgaat tacaggttgg
     6241 gatgttttct cattggctcc gggggttggt ccgccatctg gcatatcttc gggaggtgta
     6301 gggaggccat cttggatagg atgcgaatct tttatatctt ctaaaatatc attaaggact
     6361 tttctcaaca gctcaggaaa cacggctact gtatttaagt ctcttgacag tttacctata
     6421 ccaaatcctt tactatcaat agtattttta cgttgattgc gcaaactatc aaaggcaagt
     6481 tttgcctgtg cgtcaactaa atcatctagt ttgagagatg aaacagtttc attacttaac
     6541 tttaattcag caagaatatt attcttgata aatgatgata cttcagtatt gaggagtttt
     6601 tgtcccaatg ataaagcttc accagatgtg tgttgttgtg ctaatagtgc aagtctgtgc
     6661 tcaatgtttt gtcgtaattg atggagtttc ttattctttg ccgaccatgg tccaaaggca
     6721 gaatcttggg ataatttatc ttttataatt agttccgaat actgtgcgac aattttttga
     6781 agtccctttt cgttttcaaa attaagatca ttttgatgta ctaaggagta caaaacattt
     6841 gaaaattcga gaatctcgct tttggcatac gcattcattg agttggaata aatgcttctt
     6901 aacacatctt ctttccctga ggaagaaaat aaaaaatcaa gacatttctg attaaaagac
     6961 gttgcaaagt tttctttttc agacactgat gctggtgttt ttagagaagt ttgcgaacta
     7021 tggatatcgg atattttgct ttttaactcg ctgtattctg ttgatgatgg tgaagttgcc
     7081 ttatataaga atgttggcgc ttgagtatta tttacattat gcatggcgca cctcagaaat
     7141 ggagaaaaag tttatctgta tctgtacatg agcttattgt actactcaaa acctttacta
     7201 aattatcaaa aatactattg gcattactgt atttttgaac taaagtttga agattatttt
     7261 tcattgtttc atcttcggca gagaatccgg cattccatgc ctgatatttt gcattatcta
     7321 gcacaacctc gccatttcca cctagattat ctaagctttt taacatattg tctattgggg
     7381 tcatgtttat attgacaaca tatcccccgt ttttttcaga taccttgccg attgttccac
     7441 ctaattctgt aagccattta tttgcttgtt ccttactaac agtattattt gctggatata
     7501 gcggtttatc tttatatttt tccttgagct ttgtcaattc gtctttaagc gatttgactt
     7561 gtaatttcac ggagtttccg tcgttacctc cgggagagat ccagccggca agactggaaa
     7621 gaacagcgct aaaatcttga tacatttgag tatatgaact aacggcatgt tcatatactt
     7681 tcagatactg ttcattaata ttattgattg actttgcaat tttatcccac agttctctgt
     7741 gagatatcat ttcatatcca tcaagctcgg cttctttcgg ggctgaatgt aataattctc
     7801 ttgcgtcttt attaattgga tattctttct tggaaagaat atccaatagt tgagcggatt
     7861 tgtttacatc catgctaatc tgagatgaat gtaatgctat ttcttctagc gatgttttag
     7921 tcaacgtttt ttgtgagaga tctttcttta atgcctgatt tgttgttctg atattatgaa
     7981 gggtgtcgtt gagcatagta agggaactta caggatgaga actggtcgtt gtttttatat
     8041 cagaattaac tgtttcggtt gatgaaccgt tggtattgtt tgggctgaat gatgaggtgg
     8101 aaatactatt agtcagagtt gttatattca tggttatttc cttataggaa gagccatata
     8161 ttattatggc tcttggatat attaagctcg aatgttacca gcaatctgac tggctgtaga
     8221 attccttgat tggttgatgt tgtcaattat attcaataat ttttgtatta attgatttgt
     8281 cgcttgggat gcttctttag atacttggga tgcttcctct gcttgtttac tgctggcctg
     8341 actgattagt tgttcttctt cttcaagagc agatgtataa cgccctcctg atgtggatat
     8401 attaccagca acggctgatg tctccattgt tgcacggcca atgttttgtt tttgctgagc
     8461 agaaagggta ttgagctcgt aagctcttct ctgggtgtca attttatcct gcaaagaaat
     8521 atcgggagaa ctaagagaag ttttatgttc cgttgacagg gatatattat ctggtgccag
     8581 tttattttta cctaaaattt ttgtgctaga gttagtttgt ggtgaggtga tatttgtatc
     8641 tatttgttta tttaacccta atttagaacc tgcaagctct ttttcaagag attgagcagt
     8701 ggcaaggttc tttcttaagg ctcctttttg ttcgctaatc cctgaatgcg tttttttggc
     8761 accgataccc gttataccta cttgtgtgac cgctccagta atgcttgatg atagggctgc
     8821 taggccttgc cgaacaatgt tctctgcagc tgattttgta gcatcgaacg caatcaatga
     8881 caattgagag cccaatctag tttctgcagt gcggagagca gaaagtagaa cggctacact
     8941 aataattaaa gaaacagcat tagaagaaag actggaaata tccagggtgt tctctggggt
     9001 aattgtgtga ctcatttctg aaagatcttt catctttttg tcagtaaggc gagcaatgat
     9061 ttctgaatca tgctgaacct gctctgataa ctttaaaagt tgatcatcat ttaatgtagt
     9121 tgttaatacg ggatttttac cgacattaag tggaatatgt gctgctaact gctgataatt
     9181 ttgtgatttt tgtgtttcgg aagaactttg agtttgtttt gtagatatat ctgtatataa
     9241 aatcggggct gattttgtgt tttgaatttc cataacattc tccttatttg tatcaagcag
     9301 tagtttgttg caaaattgct tttgcaacat cagttctatt agcctgagag ttggacattg
     9361 aggctaacag ctctgcaatt acttcctgca attggccgaa tttttctatt gcgtcaccga
     9421 tgtattttga cagttgttca acttgatatt tcgataatgt caggtctgct agatttttcg
     9481 acgcgttgtt ttggaaaaca gcagaagcga catttcctcc cgcttgtgtg gcagagttaa
     9541 cactttctcc taataaaact gcttggttta aatgggtgga aataatttgt ttggatatta
     9601 cttcatcacc cgctgcacca agaaatttat ttaatctggc aacagcatta gtgattaaat
     9661 cgtccagttg agaagaaaaa ttcttgagaa actttggtat aaggtctgtg agggttttac
     9721 ctattatttt gccaatattc tctgcaagtt ttgctgctgc ctgtttacca acagtggcta
     9781 cgagaacgac tgctgcaacc aagacaagag cgcctgcgat tgcccccaga atagagccaa
     9841 tcattttggc ttttttcgag tcgacgccca agccttcgag catttttgta aatgcatctg
     9901 aaaggagttt gattaagggt tcaatgactg ctttcatgat cggattcagg gcttgttcca
     9961 tgaaggaatt gccggtcgct gcttgtacta tagcatccgt aaccataaga gctaaaccaa
    10021 cagctgccag tgctagagat gctcctccag aaaaagctgc tgcaacaaca ctaacgatag
    10081 ttaaaagtgc cccaagtatt ttcccaacac aacccattac tctgttgagt tcttctgctt
    10141 tacgtacttc agcagcatac tcatcagatt ttctctccat ttcagttttt cttgattctt
    10201 ggagagactg gaatagagcc agatcatttt ttaaagattc ttcattattt tttccaacta
    10261 gttgaataaa ggttgccatc aattgagtaa cactggcaag tccggttaat gatttctgct
    10321 gggttgatag ctgttcagca gatgctgtgt ttgaaaatgc agaaaaagag tctatttctt
    10381 tttcgagttg cattgattta tctgtaagtt tgctatgaat tgacagagtt ttctgctcaa
    10441 tcaatgtcct gtctttaact gctgcgtctt ttttgatagt gagttgtatt tcttcccgtc
    10501 ttaatttttt attttctggt gaatctgggg cgagttcgga taatcttgtt tgaatttggt
    10561 taattttatt ttctaggtct tttatttttg aatctgcgtt ttttagttta ttaatttgtt
    10621 tttcatagtc tctggttagt ccttcagttt cagatagaag agtattaatt ttatcggaga
    10681 attctaggtt tttttgctgt cttgcctgtt gctgggactt ccaggcagta attttatttg
    10741 ttaatgcagt taaagatttt tcaccaagta tttgaataag gtttccaatt aaaagcgtta
    10801 gttgggaact tgcatttaat gactttggtg ctttaagttc agggattaat atattcgaag
    10861 ttgagtgtgc attagttgta ttaatatttt tgttagcagc aagatcagca attgtaagag
    10921 aaaataattt gttagctgca tcatttgcag cctggatagt attgtctcca agctcagtgg
    10981 aagccaatat tttggcaaga ggcaaaccag tggttgtggt gcttacatta tgcataataa
    11041 ttactccttg atatcctgaa ttgcgtctaa gtatgattgt gcttttattt ttaatttttc
    11101 atcattgctg tgttgaatta cgagttcgaa gcactctttg gctttcaagg gggctttcaa
    11161 ccgaagctgg cattgtccag tatggaatac cggtgtatag tcattttttc ctaatgcaaa
    11221 agcgacagca taaaggtctg ctgcttgttg gaactgttct tttatctgat aaatagctgc
    11281 gagtcccata atgtagtcta cattgtaaaa gtcgtatata cataaaaatc tgaagaaaac
    11341 ttcagcttcc tctattcttc ctttgttgta aaagtcataa gcatatgaat aaatgtcatc
    11401 catcatatca tcaggaattg cattaatatc tttcagtgta gcgccagagt taattgcatc
    11461 aattactgca gtagagatgc tttcattttc agtgatattt aaagacataa ggtctccttt
    11521 ttgtctgcat tactttaata agtataagat ttaatataaa cagatttaat ttgtattgct
    11581 ttgacggtat acagccttta gaacctctgc gcttatttct atttgtaaaa cttttgttga
    11641 ttcattaaat attttatcgt agacggaact ggatactaga tttgtaagat ccttcatttg
    11701 tgtgttaaac aaatccatgt cactgctata acgttttaaa atctttgctc tgaggccaga
    11761 tgaggcaatc tcgtttagtg ctgtatgact gatagatgtt tgtgctgatt tttcatcaag
    11821 ttttatatcc gggaattttt tttcaatagc tgcaaatatg ttttttttgt tatctgaggt
    11881 caaaacaact ccattgtgta tggccatttc atctatactt ctgtttatca ttgaagagac
    11941 ataagaggtt cgctcttggc tcatccaaca aaatagttga ttccccgaat tactatcttt
    12001 tattttgtct atgacgctgt ccctgatttt atttatattc accgggcatc caaaattctt
    12061 ctgaggaaag cgttgtttta agttaaaaac agataatatt ttctttacaa gcttattgtg
    12121 acttaacgaa tcaaatgatg ggcgaggaat cgcgaattca acctgtggaa gtattttgtt
    12181 tagaatttgc atgatacccc ctatatgtta gttcacttct gaagtgatgt ttgcaatggt
    12241 tagtttaata caaaataaaa attcgtacaa agattcaatg agttcatcag gtgaaagata
    12301 ttttttgtag tgtttcttgg tcaaaacaca ttgatgagta tcagggagaa gacagataga
    12361 agaaccataa tctctactca aagagtagtt aaggatgagt atgtgttctg gattaataat
    12421 attttctgta aaaagttcat gttcattaag aagtccggtc aaaaagatgt attcatcatt
    12481 atctgttata caaacaggta tttgttcttc gagcaatata aaggcttgat ttttatcgtt
    12541 aaaatcaagc tttgcacctg ttatttcata aagtttatca tatagtttgc gacacatttc
    12601 tatggccttc tatatattag aatgagagtt attcaataaa agcttattga cctgtaataa
    12661 gtcgtgaatg taaagtttgg ttccaagatc tggcgattta agagaattgt aataatcaat
    12721 agtttttatt gacttttcga taattacttc ttttttatct gttgctccgg tttttaattc
    12781 gtttaataca cgagacatat tgctgggtgc tgattttact aagtatgagt ggcaagcgtt
    12841 gatgaccgca tcaaagtctt ctgaagatac attttcagta ggatgtttgt aaagtgaaat
    12901 tgataaaggc ttgaatgttt ttggggtgtg gataaggtta attttattga aacttttaag
    12961 atagctacgt cgtaattgag tcaatctgga aagatcaaaa tcagattcaa tatacttcat
    13021 tatctcgtta taagactgat tgagactttc aatgcgttgc ctaactttat ttgcatacgc
    13081 gtatacattg ttaggtgtag caaccaattt aacgtcaggg agtggaaagt atatttcaga
    13141 atttcctgct ttaagaacat taagtgccat accagcacag ttttcagatt tactgattaa
    13201 tttataattt gccaattgtc tgaaagcatc tgctttcttt tggcatataa attttgacat
    13261 gtttgtttca tcaaggccaa aaaggttata gctgatttta ccatcctttg ttttattatc
    13321 acctgataat ggaatgtata ctttatcagc actgaccccc caataaccat aggggtcttt
    13381 tttttgatta gctctgggaa aaaatatttt tttgttgtta ttttgatctt gttttgaatt
    13441 taataagttt ctaacctctt ggcctgcatc aagtctttcg gctgtttggt ctgaaatcat
    13501 atttagtttg tcttgtttat aggtatcgac actgtaacct gagcgttttt caaaaaggta
    13561 gtttatttct gtatcttttg taacattccc gccgggatac cagctgagat aggtatgagt
    13621 tggttcctgt agtaaattgt tttttatact gatactggta tgccctaaat gggttatgcc
    13681 tctatttccc cagcaacgaa acactaaatg tctttcaaga tttttggctg taccgatgcc
    13741 atgaaaactc tctgcggtat tcaacattaa tttgatgtct ttacctgtaa aagaatgttg
    13801 aattgaattt ttgactgctt cataggcggc aaacgcactt tctgcagatt tttcgctagc
    13861 taatgtcttg aggaaaagca ataaggattt ttgattcgct agtcgataat tttttagttg
    13921 ttcatttaat atctgtgaat ttatctttgc attaatagtg cttaatgttc tgaaaaagag
    13981 atcctttagg ttgaatttct gtgctaccaa tataggtcca tgctctgtat cttcaacgcg
    14041 tataggccct tttgtattgc ttgcgtcaat gaaattgcta attttgagaa tcatacttta
    14101 ttaactctcc attacgtggt gattgtgggg atgtaaaatg tatttgatat ataatatatt
    14161 caattaataa atttagaaac ttgagcctgt taacataatc aaattttctt ttgctgtaca
    14221 taatatgtac ctcgtgagca tatgtagtgc tggtttcatc atgaaatccc acaagttaaa
    14281 gtgtctgata tatcaggctc ggagtgttat agaaaaagag agaaccctgt tgaataagta
    14341 tcttttttgt tgtaaaacga ggtacaaaac tgcaatcaat gccccattta tgactgagga
    14401 taattaaatg cacataacta atttgggatt gcatcaggtt tcatttcaaa gcggagattc
    14461 ctataaaggc gcagaagaaa ccggaaacca aaaaggtgta agcgtgattt catatcaaag
    14521 agttaaaaat ggagagagaa ataagggggt cgaagcgctt aatcgattat atttacagaa
    14581 ccaaacaagc ttaacaggga aaggcctttt gtttgcgcgt gacagagctg cagtttttta
    14641 cgaggcaata aagttggcag gtggtgatac gtcaaaaatt aaagccatga tggaacaatt
    14701 agatacctat aaacttggtg aagttgataa aagaaatatt aatgagctta ataaagtaat
    14761 aagtgaagag ataagagcac agctaggcat taaaaataaa aaggaattgc aatcggaaat
    14821 aaaacaaata tttactaatt atttaaataa taagaattgg gaaccgatca acaaaaatat
    14881 taattataat gggaaaaatt atggttttca attaacaccc gcctctcata tgaagatagg
    14941 gaataaaaat atatttgtca aagagtataa tggaaaagga atttgttgtg cttctacgag
    15001 agagagtaag catattgcga atatgtggct ttcaaaagtg gtggatgacg aggggaagga
    15061 aattttttcg ggtatcagac atggagttat atcagcctat ggattgaaaa aaaactcatc
    15121 tgaaagagct gttgcagctc gtaataaagc tgaggagtta gcaagcgcag cattatatag
    15181 cagacaagaa ttattatcac aggctttgtc tggtaaaaca gtagatttaa agattgtttc
    15241 gacttctctt ctgacgccga ccagtttaac tgggggagag gagagtatgt taaaagatca
    15301 ggtaaaggca ttaaaagagt taaactctaa aaggggggaa ccaacgaaat tattaattcg
    15361 gaatagtgat ggtcttctgc aagaagtaaa ggttaatcta aaagtggtga catttaactt
    15421 tggtgtaaat gaattagcgc taaaaatggg cttaggctgg aggaatgttg acaagcttaa
    15481 tggtgaatca atatgttctt tgttggggga taatttcctc aaaaatggtg tgattggagg
    15541 ttgggctgca gaagctatag aaaaaaatcc accatgtaag aatgatgtga tatatctggc
    15601 taaccagata aaagagattc taaacaagaa gttacaaaaa aatgataatg gagagccgta
    15661 taaattgtca caaagaatgg ctctattggc ttatactata ggtgctgtac cttgctggaa
    15721 ttgtaagagt gggaaggaca gaacaggtat gcaggatgct gaaattaaaa gagaaataat
    15781 aagaaaacat gaaacagggc agttttctca attaaatagt aaattatcct cagaagagaa
    15841 aagattattt tctactattc taatgaatag tggtaatatg gaaatccaag agatgaatac
    15901 tggtgtgcct ggaaataaag ttatgaaaaa attaccgcta tcttcgttag agctgtctta
    15961 tgctgaaaga ataggggacc ccaaaatatg gaatatggtg aaagggtatt cgtcatttgt
    16021 ataagaggaa tatatggaag atttagcaga tgttatttgc cgagccttgg gtatcccttc
    16081 aattgatacc gatgatcaag caatcatgct tgatgatgat gtgttaatat atattgaaaa
    16141 agaaggagat tcaataaatt tattatgtcc tttttgtgca ttgcctgaaa atataaatga
    16201 tcttatatat gcgttgagcc taaattactc agaaaagata tgtttagcta ctgatgatga
    16261 aggtggaagt ctaatcgcgc gattagatct taccgggata aatgaattcg aggatatata
    16321 tgttaataca gaatattata tttcgcgtgt gcgttggctg aaggatgaat ttgcgcgaag
    16381 aatgaagggg tattaatgtc ccgtttcgta tttatttttc tgtgttttat cccatattta
    16441 gggagagctg attgttggga taaggctggt gaaaggtaca acattccatc gagtttgtta
    16501 aaagcgattg cggaaaaaga atccggattt aataaatttg cagtcaatgt taataacaat
    16561 ggaagtaaag attatggtat aatgcaaata aatgactttc attctaaaag acttagagaa
    16621 atgggatata ctgaggaaat gctaattagt catccatgtc tttctgtaca ttatgcagca
    16681 aagttattaa atgagtttat gctgatgtat ggaagagggt gggaggctgt tggtgcctat
    16741 aatgcaggaa catctccgaa aaagaaaaaa gaaagattaa agtacgcaga ggatatctat
    16801 aaacgctatt tgcggatagc agcagagagc aagcagaata atcgaaggat ataagaggat
    16861 ttatgtctga ggcaaagaac tcaaatcttg caccattcag gttattagtg aaactcacaa
    16921 acggtgtcgg ggatgagttt ccgttatatt atgggaataa cttaattgtt ctcggacgga
    16981 cgatagaaac acttgagttt ggaaacgata atttcccaga aaatattatt ccagtaacag
    17041 atagtaaatc ggatggtata atttatttgg ccataagtaa agataatatc tgtcaattta
    17101 gtgatgaaaa aggtgaacaa attgatatca attcacaatt taactcattt gaatatgacg
    17161 gagtttcttt tcaacttaaa aatatgatgg aggataaatc gagagttcat atactaaacg
    17221 gaatgtataa taaaccctcg gtgttttttt tctttgctgt tattgttgtg ttaattataa
    17281 ttttttccct ctctttaaag aaagatgaag taaaagaaat tgctgaaatt atagatgata
    17341 agagatatgg aatagtcaac acaggacagt gcaattatat tcttgccgaa acacaaaatg
    17401 atgcggtatg ggcatctgta gccttaaata aaagcggatt taccaaatgc agatatattc
    17461 tggtgtccaa caaagaaata aatagaattc aacaatatat caatcaacga ttcccatata
    17521 tcaatttgta tgtgttgaat ttagtgagtg ataaagcgga attactggta tttttaagta
    17581 aagaaagaaa tagttcaaaa gataccgaac ttgagaaatt gaaaaatgct ctgatagttg
    17641 aatttccgta tatcaaaaat attaaattca actatcttag cgatcataat gcaagggagg
    17701 atgctaaagg tattttcaca aaggtaaatg tccagtataa agagatttgt gaaactaata
    17761 aagttacata ttctgtcaga gaagaactga cagatgaaaa gcttgagctc attaatagac
    17821 tgatttctga tcataaaaat gtatatggag atcagtatat tgagttttct gtacttttga
    17881 tagatgatga ttttaaaggt aaatcatatc ttaacagcaa agacagttat gtaatgttga
    17941 atgataaaca ctggtttttt ttagataaaa ataagtgagg ataaaatgag tgttacagta
    18001 ccggataaag attggactct gagttcatta tctgaaactt ttgatgatgg aactcaaaca
    18061 ttacaagggc aactaacatc ggcactaaat gcattagctg aaaatccttc gaatccacag
    18121 ttgctggctg aataccaaag taaattatct gaatatacat tatataggaa cgcgcaatct
    18181 aatactgtga aagtgattaa ggatgttgat gctgcaatta ttcaaaactt cagataatag
    18241 ggagcattca tgaattacat ttatccagtc aatcaggttg atattatcaa agccagtgat
    18301 tttcaatctc aagagatatc aagcctggaa gacgtcgtgt cggctaaata tagtgatatt
    18361 aagatggata cagatattca agtatcacaa ataatggaga tggtaagcca tccagaatca
    18421 ttaaacccag aatctttggc caagttacag acgacgctct caaattattc aataggagta
    18481 tcattagctg gcacgttagc aagaaaaaca gtttcggctg ttgaaacttt attaaagtct
    18541 taatttatat gattaggtat aaatgtttta ttttattctt gttgctgatg ttgattggat
    18601 gtgagcaacg tgaagagtta atttctaatt tatctcaaag acaggcaaat gaaataatat
    18661 ctgtgctaga acgccataat attactgcta gaaaagttga tggaggtaaa caggggatct
    18721 cggtacaagt cgaaaagggg acatttgcat cggcagttga tttgatgcgc atgtacgatt
    18781 tgccaaatcc ggagagagtt gatatctcac aaatgtttcc tacagattca ttagtgtctt
    18841 ctccaagagc tgaaaaggcc cgtttatata gtgctattga gcaacggctg gaacagtctt
    18901 tagtttctat tggtggtgtt atttcggcaa aaatacatgt tagctatgat cttgaagaaa
    18961 aaaatatatc ttcaaaaccg atgcatatat cagtaatcgc tatatatgac tcaccgaaag
    19021 agtctgaact attagttagt aatattaagc gatttttgaa aaacaccttt tctgatgtta
    19081 agtatgaaaa tatatctgtc atattaactc cgaaagaaga atatgtttat acaaatgtac
    19141 aacctgttaa ggaaattaaa tcggaatttt taacaaatga agtaatatat gtatttctcg
    19201 ggatggctgt actagttgtc attcttttgg tatgggcatt caaaacaggg tggttcaaga
    19261 gaaacaaaat atgataagaa tggatggaat ttataaaaaa tatctttcaa taatttttga
    19321 tccagcgttc tatataaata gaaatcggtt aaatttgcct tctgaactgt tagaaaatgg
    19381 cgtaatcaga agtgagatta ataatctcat aattaacaaa tatgatttaa attgcgatat
    19441 tgaaccttta agcggggtaa ccgctatgtt tgttgccaac tggaatttac ttccagctgt
    19501 tgcttatttt atcggtagtc aagagtctcg gttaattaat catagtgaga tggttattag
    19561 ttattatggg ggcaaaatca gtaagcaagg ggaggccgct attcgaagtg gtttttggca
    19621 tcttattgag tggaaggaaa atatatctgt tggtatatat gaaagaataa acttattatt
    19681 taatccaatt gctttggagg gaaattatac gcctgtagaa agaaatttgt cccgattaaa
    19741 tgaaggtatg caatatgcaa aaaggcactt taccggtatc cagacatcat gcctatgatg
    19801 gtgttgttat aaagagaatt gaaaaggaat tatgtaaaac cataaaagat agagacactg
    19861 agagtaagaa gaaagcaatt tgtgttataa aggatgctac gaaaaaagct gaatcactgc
    19921 gtatagatgc tgtatgcgat ggttatcaaa ttggtataca gactgctttt gagcatatta
    19981 ttgactatat ttgtgaatgg aagttaaagc aaaatgaaaa cagaagaaat attgaggact
    20041 atataactag tttgctttca gaaaatttac atgatgagag gatcattagc actttattgg
    20101 agcagtggct tagttcgttg cgtaatacag taacagaatt aaaagtggtt ttgcccaagt
    20161 gtaatttagc actgaggaaa aagttagaac ttgatctcca taaatatcgc tcagatgtca
    20221 agattatttt aaaatattct gaagggaata attatatatt ctgtagtggt aatcaagtag
    20281 tggagtttag tcctcaggat gtaatatcag gtgttaaaat agaacttgcg gaaaaattaa
    20341 caaaaaatga taaaaaatat ttcaaagagt tagcacataa gaaactgaga caaattgcag
    20401 aagacttgtt aaaagagaat cctgttaatg attaatcaaa taaatgcaag caatgctctg
    20461 cagcaaagat taaatagtga agaatttgtt aatttaaatg agagattatc atcttcgcaa
    20521 tcatttgatg agaatataat ctatgaaata atgcaatatt tctcacagag tgaattaaat
    20581 tccatagata atgatgaact gcacaataag attgaacaat tattcaattc acgttttcct
    20641 tatcttacag ctgcacaaaa aagttcactt cttaataaac tcatagatgc aaatcaatat
    20701 gtcgatttgc atgagggatt ttatgcatca ttgtccatat ataacaatat tgatttctat
    20761 attaagacta caaccttcga ttcattaatt agtgtctttg aagcagggag agaggcagat
    20821 gattcgacat ggtagtaata agttgaaaat atttatttta agtatattgc tattaacact
    20881 gagtgggtgt gctttaaagt catcatctaa ttctgaaaaa gaatggcata ttgttcctgt
    20941 aagtaaggat tatttttcta ttccaaatga tttattatgg tcgtttaata caaccaataa
    21001 aagtataaat gtttactcta aatgtattag tggtaaggcg gtttatagtt ttaatgcagg
    21061 taaattcatg ggcaacttta atgttaagga agtagatggg tgcttcatgg atgcacaaaa
    21121 gatagctata gataaactat tttctatgct gaaagacggg gttgttttaa aaggtaataa
    21181 gataaatgat accatcctta tagagaagga tggggaagtt aaattaaaat taattcgagg
    21241 gatataattg tattgtgagt aaatataaag gtctaaatac aagtaatatg ttttacattt
    21301 actctagtgg acatgaacca gttaacgttg agcttgtaaa agataaagaa cgtaacataa
    21361 ttgagctggc tccagcatgg aagggttttt ttttgtccga aatcaaaata taaaattcag
    21421 tgataatgtg aactatcatt atcgctttaa cattaatagt tgtgcaaaat ttcttgcttt
    21481 ttgggactat ttcagtggtg ctctagtaga gcatagccat gctgaaaaat gtattcattt
    21541 ctatcatgag aatgatcttc gggatagttg taataccgaa tcgatgcttg ataagttaat
    21601 gttaagattc atcttttcat ctgaccagaa tgtaagtaat gcactggcta tgatacgaat
    21661 gactgagtca tatcatttgg ttctttatct tctgagaact attgagaaag aaaaggaggt
    21721 aagaataaag agtctgacag agcattatgg tgtgtcggag gcttatttta gatctttatg
    21781 tagaaaagct ttaggggcaa aagtcaaaga acaactgaac acttggcggt tagtaaatgg
    21841 tttattagat gtatttttgc acaatcaaac aatcacttct gctgccatga ataatgggta
    21901 tgcatcaaca tcccattttt caaatgagat aaaaacacga ctaggctttt ctgcaagaga
    21961 attatcgaat attacttttt tagtgaaaaa aataaatgaa aaaatttaat attaaatctt
    22021 tgactctctt gattgtattg ttaccattga tagttaatgc taataatatt gactctcatt
    22081 tattagaaca aaatgacata gcgaaatatg ttgctcaaag tgatacggta ggatcttttt
    22141 ttgaacgttt ttctgcgtta ttaaattatc ctattgttgt tagcaaacaa gctgcaaaaa
    22201 agagaatatc tggggagttt gatttatcta atccagaaga aatgcttgaa aaaatgacat
    22261 tacttgtggg actcatatgg tataaagatg gaaatgcatt atacatatat gactctggag
    22321 aattaataag caaagttatt ttgctggaga atatatcact taattatctt attcagtatc
    22381 tgaaggatgc taacctttat gatcacagat atcctataag aggaaacatt agtgataaaa
    22441 cattctatat atctggtcct ccggcattgg ttgaactggt agctaataca gcaactttgc
    22501 ttgataagca ggtttcttct attgggacag ataaagttaa ttttggggtt ataaaattaa
    22561 aaaatacatt tgttagtgac agaacttata atatgagagg ggaggatatt gtcatcccag
    22621 gggtggctac tgttgtagaa cgtttattaa ataatggtaa ggctctgagt aacagacaag
    22681 ccaaaaatga tccgatgcca ccctttaata ttacacagaa agtatctgag gatagtaatg
    22741 acttttcatt tagttcagtt accaatagtt ctatattaga ggatgtatca cttattgcat
    22801 atccagaaac caattctata ttagtcaaag gtaatgatca gcaaatacag attattcgcg
    22861 atattatcac tcagttagat gtagccaaac gacatataga attatcatta tggataatcg
    22921 atattgataa atctgaatta aataaccttg gcgttaattg gcaaggaacg gcttcatttg
    22981 gtgattcgtt tggtgcatca tttaatatga gctcatcagc atcgataagc actttggacg
    23041 gcaataagtt tattgcttca gttatggcat taaatcagaa aaagaaagca aatgtagttt
    23101 caaggccggt gattttgaca caagagaata ttccggctat atttgataat aacaggactt
    23161 tttatgtgtc attagttggt gagcgcaatt cttcgttgga gcatgttacg tatggaacat
    23221 tgataaatgt tatacctcgc ttttcatctc agggacaaat tgagatgtct ttaacgatag
    23281 aggatggaac aggaaatagt cagtcaaatt acaattataa taatgaaaat acatctgtcc
    23341 ttccagaagt tggaaggacc aaaatcagta caatagcgag agttccacaa ggcaaaagtt
    23401 tgttgatagg tggttatacg catgaaacga actctaacga aatagtatct attccttttc
    23461 tctcctctat tcctgtaatc ggtaatgtgt tcaagtataa aacaaggaat atcagtaata
    23521 ttgtaagggt ttttctgatt caaccccgtg agataaagga gtcaagttat tataatactg
    23581 cagaatataa gagcctcata tctgaaagag aaatacaaaa gactacacaa ataatcccat
    23641 ctgaaactac tttgctggaa gacgaaaaat cattggtttc atacttaaat tactaactat
    23701 aaagtaggtg atgtatgctt gatgttaaaa atacaggagt ttttagctct gcattcattg
    23761 ataagttgaa tgcaatgaca aattcagatg atggagatga gactgctgat gcagagcttg
    23821 attctggctt ggctaatagc aagtatattg actcatctga tgagatggct tccgctcttt
    23881 cgtcatttat aaacagaaga gaccttgaga aactgaaagg aacaaatagt gatagtcagg
    23941 aacgtatttt agatggggaa gaagatgaaa ttaatcacaa gatttttgat ttaaagagaa
    24001 ctttaaaaga taaccttcct ctagatcagg attttataga cagactaaag agatatttta
    24061 aagatccaag tgatcaagtc ttagcattaa gggaactttt gaatgaaaaa gatcttactg
    24121 ctgaacaagt cgaatcatta actaaaatta ttaatgagat aatatcaggt agtgaaaaaa
    24181 gtgttaatgc tggaataaat tcagctatac aggctaaatt atttggcaac aaaatgaaac
    24241 ttgaaccaca gcttttgcgt gcatgttatc gtggttttat catggggaac atatcaacaa
    24301 cagatcagta tatagaatgg cttggtaatt ttggttttaa tcacagacat acaattgtga
    24361 attttgtaga gcagtcactg attgtagaca tggattctga gaaaccgagc tgtaatgctt
    24421 atgagtttgg ttttgtgtta tctaaattaa ttgcaattaa gatgattaga acttcagatg
    24481 taatttttat gaagaaactg gaatcctcaa gcttgctaaa agatggcagt ttaagtgcag
    24541 agcagctatt gctaacttta ttatatattt ttcaatatcc aagtgaaagt gagcaaattc
    24601 ttacttctgt tatagacgta tcacgagcca gtcatgagga ttctgtagtg tatcaaacat
    24661 atctatcttc tgttaatgaa agtcctcatg atatatttaa aagtgaaagt gaaagagaaa
    24721 ttgcgatcaa tattctacga gagcttgtca caagtgcata caagaaagag ctttctagat
    24781 aacaggagat gaaagtgatc cagtcttttc ttaagcaagt aagtactaag cctgaattaa
    24841 ttatattagt gcttatggtt atgatcattg ctatgttgat cataccatta cctacatacc
    24901 ttgttgattt tttaataggg cttaatattg tacttgctat tctcgttttt atgggatcat
    24961 tttatattga aagaatattg agtttttcta cattcccttc ggttttgttg ataacgacat
    25021 tatttcgtct cgctttatca ataagtacta gtcgactaat tttagttgat gcggatgcgg
    25081 gtaaaattat tactacattt ggccagtttg tcattggtga tagtctggct gtaggttttg
    25141 ttattttttc aatagtaact gttgtgcagt tcattgttat aacaaaagga tctgaaaggg
    25201 ttgcggaggt agctgctcgc ttttctcttg acggtatgcc ggggaaacaa atgagcattg
    25261 atgcggattt aaaggctgga attattgatg ccgcaggagc taaagaaaga cgcagtattc
    25321 tggaacgtga gagtcaattg tatggttcat ttgatggagc aatgaagttc attaaaggtg
    25381 atgcaattgc tggcatcatt attatttttg tcaatttaat aggaggtatt tctgttggta
    25441 tgagtcaaca tggaatgtct ctatctggtg cattatctac ttataccata ctaactattg
    25501 gtgacggatt ggtctctcag attcctgctc tgttaatttc tataagtgca ggatttatcg
    25561 ttacaagagt taatggtgat agcgataata tgggacgtaa cataatgtcc cagatatttg
    25621 ggaatccttt tgttctcatt gttacatcag ctcttgcctt ggcgataggg atgttgccag
    25681 gttttccgtt ttttgttttt ttcctgatag cagttacttt gacggcttta ttctattata
    25741 aaaaggtcgt agaaaaagaa aaaagtctgt ctgagtctga ttctagtggc tatactggta
    25801 catttgatat tgataatagc catgactcat ctttggcaat gatagaaaat ctggatgcta
    25861 ttagttcaga aaccgttcct ttaattttat tatttgccga aaataagata agtgcaaatg
    25921 atatggaagg tcttattgaa aggataagaa gtcagttctt tattgattat ggtgtcaggc
    25981 ttccaactat tttatataga acaagcaatg agcttaaggt cgatgatatt gttttgttaa
    26041 taaatgaggt gcgtgccgat agttttaata tatactttga caaagtgtgt attacagatg
    26101 aaaatggaga tatagatgct ctaggtattc ctgttgtttc aacttcatat aatgaacgtg
    26161 ttatttcttg ggtagacgtt tcatatacag aaaatctaac taatattgat gctaaaatta
    26221 aaagtgctca agatgaattt tatcaccagt tgtcacaagc tttattaaac aacataaatg
    26281 agatttttgg tatacaagaa acaaaaaata tgttagatca gtttgaaaat cggtatcctg
    26341 atctattaaa ggaagtcttc cgacatgtga ctatacagag aatttctgag gtattacaaa
    26401 gattgcttgg agaaaatatt tctgttcgca atttaaaact tattatggag tctttggcgc
    26461 tttgggctcc cagagaaaaa gatgtcataa cattagttga acatgtccgt gcatcacttt
    26521 ctaggtatat ttgtagtaaa atagctgttt ctggtgagat taaagttgtg atgctttccg
    26581 gatatattga ggatgcaata agaaaaggga taaggcaaac ctctggtggc tctttcttga
    26641 atatggatat agaggtttcg gatgaggtaa tggaaacttt agcacatgct ttgagagaat
    26701 tgagaaatgc aaaaaaaaat ttcgttcttt tggtatcagt agatatacgt aggtttgtta
    26761 aaagacttat agataacaga tttaagagta tactcgttat atcgtatgct gagattgatg
    26821 aagcatatac cattaatgta ttaaagacta tttagtgagg tttaaatatg agtaacatta
    26881 atttagttca attagttaga gatagtcttt tcacgattgg ttgtcctcca tcaattatta
    26941 cagatttaga cagccattca gcgattacca tttctttgga tagtatgcct gctattaata
    27001 tagctcttgt caatgagcag gttatgttat gggctaattt cgatgcacca agtgatgtaa
    27061 aacttcagtc atctgcttat aacatattaa atttaatgtt aatgaacttt agttatagca
    27121 ttaatgagct ggtagagctt catcgttctg atgagtactt acagttaaga gttgttatta
    27181 aagatgacta cgttcacgat gggattgttt ttgcagagat tctgcatgag ttttatcaga
    27241 ggatggaaat cttaaatgag gtcttataat caatgagcta tataaaattg ctcactcaat
    27301 tatcttttcc taatagaatc tcggggccaa tcttggaaac aagtcttagc gatgtttcga
    27361 ttggtgagat ttgtaacatt caggctggaa ttgaaagtaa tgaaattatt gcaagagctc
    27421 aggttgtagg atttcatgat gaaaaaacaa tattaagctt gattggaaat tctcgtggac
    27481 tttcaaggca aacgttgatt aagcccactg cccagtttct tcatacgcaa gtcggccgtg
    27541 gattattggg agcagtagtc aatcctttag gggaggttac tgataagttt gctgttacgg
    27601 ataacagtga aattctttat cgacctgtag ataatgctcc tccgctatat agtgaaaggg
    27661 ctgcaattga gaagcctttt ttaacaggtg ttaaggttat tgattcttta ctcacgtgtg
    27721 gtgaaggaca gcgaatgggg atttttgcgt cagctggttg tggcaaaact tttctcatga
    27781 atatgctcat tgaacatagt ggtgctgata tatatgttat tgggttaatt ggtgagcgag
    27841 gtcgagaggt tacagaaacg gttgattatt tgaagaactc tgagaaaaaa agcaggtgtg
    27901 ttttagtata tgcaacttcg gattactctt cggttgatcg ttgtaatgct gcatatatag
    27961 ccactgctat agccgaattt tttaggactg aaggacataa agtagcgctt tttattgatt
    28021 cattaacaag gtatgccaga gcattacgtg atgtggcctt agccgctgga gaatcacctg
    28081 ccagaagagg ctatccggtt tcggtttttg atagcttacc cagacttctt gaaaggccag
    28141 gaaagttaaa ggcaggtggc tctattactg cattttacac tgttcttttg gaggatgatg
    28201 attttgctga tccattagct gaagaggtaa gatccatttt agatggacat atatatttga
    28261 gcagaaatct agcccaaaaa ggacaatttc ctgcaattga ttccttaaaa agtataagca
    28321 gggtatttac acaggttgtt gatgaaaaac atcgtattat ggccactgca tttcgggagc
    28381 tactttctga aatagaagag ctaaggacaa ttattgactt tggtgaatac aaaccggggg
    28441 agaatgcctc tcaggataaa atctacaaca aaatatctgt tgttgagagt tttctgaagc
    28501 aagattatcg actcggtttt acttatgagc agacaatgga gcttcttggt gaaacaatta
    28561 gataaggttt taaaaatcaa agataaatat caacggtctg ttaaattaat tgaagcacac
    28621 atattaacac taaaaaaaaa actctttgta tcgagatgtg gaggcattag ataaaaggat
    28681 tatatacttc ctgcaactgg agaatgactt ggaacctgta ggcgctcaga gtgttagtca
    28741 gcttttcaat acaagacgta agatcgctat agtaaaaaag catattatcc aatatcagtc
    28801 tgaacgaatt cttctaaagg gaagaattga agaaattcaa aaagatattg atgaggcaaa
    28861 tgcttctaaa agaaaactgc ttcataaaga gagtaaaata tgtaaacgga ttggcttgat
    28921 taaaagaaat aactttgcca aacagttgat acttgatgag ttatcacaag aggatatgaa
    28981 gtatggcatt agataatata aacctaaatt ttagtagtga caaacagata gaaaaatgtg
    29041 agaaactatc ttcaatagat aatattgata gtctcgtttt gaagaagaag aggaaggttg
    29101 aaattcctga gtactcttta attgcatcta actattttac tattgataag cactttgaac
    29161 ataagcatga taaaggagaa atttatagtg gcattaaaaa tgcgttcgaa cttagaaacg
    29221 aacgagcgac atattctgat attccagaat caatggccat taaagaaaat attttgatac
    29281 cagatcaaga tatcaaagca agggaaaaaa taaatatcgg cgatatgagg gggatctttt
    29341 catataataa gagtggaaat gcagacaaga acttcgaaag aagtcatact tcttctgtaa
    29401 accctgataa tctgctagaa tctgataata gaaatggtca aattggttta aaaaatcata
    29461 gcttgtctat tgataagaat attgctgaca tcatttcttt actaaatgga agtgttgcta
    29521 aatcatttga gctgcctgta atgaataaaa atactgcaga cataacccca tccatgtcat
    29581 tgcaagaaaa atcaatagtt gaaaatgata aaaatgtttt tcaaaaaaat agtgaaatga
    29641 cttaccactt taaacagtgg ggggctggac attctgttag tatttcagtg gagtctggtt
    29701 cttttgttct aaaaccgtca gatcaatttg taggaaataa acttgactta attttgaaac
    29761 aagatgctga gggtaattac agatttgata gcagtcaaca taataagggg aataaaaata
    29821 atagtacagg atataatgaa cagagtgaag aagaatgcta agaattaaac attttgacgc
    29881 taacgaaaaa ctacagattt tatatgcaaa gcaactctgc gagcgttttt cgattcagac
    29941 attcaaaaat aaatttacag gcagtgaaag tttagtcact cttacttccg tgtgtgggga
    30001 ttgggtaatt cgtattgata cattatcttt tttgaaaaaa aaatacgagg tattttcagg
    30061 attttctaca caagaatctt tactgcattt atcaaaatgt gtctttatag agtcgtcatc
    30121 tgtattttcg attccagaac tgtctgataa gattactttc cggatcacga atgaaatcca
    30181 gtatgcaact actgggagtc atttatgctg tttttcctct tctttaggta ttatttattt
    30241 tgacaagatg ccggtattac gtaatcaagt ttctcttgac ttattgcatc atcttttaga
    30301 gttttgctta ggttcatcta atgtaaggct ggctacttta aaaagaattc gtactggtga
    30361 tataatcata gttcagaaac tttataattt attattgtgt aatcaagtta ttattgggga
    30421 ttatattgtg aatgataata atgaggcaaa aattaatctg tcagaaagta atggtgagtc
    30481 agatcacaca gaagtttctt tggcattatt caattatgat gatatcaatg taaaagtgga
    30541 ctttatcctt ttagaaaaaa agatgacaat caatgaacta aaaatgtatg tagaaaacga
    30601 attattcaag tttcccgatg acatagttaa acatgtaaat attaaagtaa atggttcttt
    30661 ggttgggcat ggggaacttg tttctattga ggatggttat ggtatcgaga ttagttcttg
    30721 gatggtaaag gagtaatctc atgctgagtg atatgtccct catcgctaca ctttcttttt
    30781 ttacgctatt accattttta gttgctgctg gtacttgtta tataaagttt tctattgttt
    30841 ttgtaatggt acgaaatgca cttggtttgc agcaggttcc atcaaatatg actctcaatg
    30901 gtattgcatt gattatggct ttgttcgtga tgaaacccat tattgaagct ggttatgaaa
    30961 attatctaaa tggcccgcaa aaatttgata caatatcaga tattgtccgt ttttctgatt
    31021 ctggcttgat tgaatacaag caatacttga aaaagcatac agatcttgag ttggcacgtt
    31081 ttttccaaag gtctgaagaa gaaaatgctg atttaaagag tgcagagaat aatgattatt
    31141 cattgttttc cctcttgcct gcatatgcgc taagtgaaat taaagatgcg ttcaaaatag
    31201 gtttttattt atatttgcca tttattgttg ttgatcttgt tatttcaagc attttacttg
    31261 cacttggtat gatgatgatg agtccaatca caatatcagt accaataaaa cttgtattat
    31321 ttgttgcgct tgatgggtgg gggatcctgt ctaaggcctt gattgaacaa tatatcaatg
    31381 ttcctgctta gatgatggag gtaatgtgtc tgatatagtt tatatgggta ataaggctct
    31441 ttatttaatc cttatctttt ccttatggcc agtaggtata gctacggtta ttggattaac
    31501 tattggttta ttacagacag tgactcaact tcaagagcag acacttcctt ttggtataaa
    31561 gcttataggt gtctcaatat ctttgctact tctttctgga tggtatggtg aggttttatt
    31621 gtctttttgt catgaaataa tgtttttaat taagagtggg gtttgatgga catttcaagc
    31681 tggttcgaaa gtattcatgt gtttttaata ctcctgaacg gcgttttttt tagattggct
    31741 ccattatttt tctttcttcc atttttaaat aacggtataa tttctccatc tattagaata
    31801 cctgtgattt ttcttgttgc atcaggatta attacttctg gtaaggtaga cataggttct
    31861 tctgtttttg aacatgttta tttccttatg ttcaaggaaa taattgttgg cctccttctc
    31921 tctttttgct tgtctcttcc cttttggata tttcatgctg ttggtagtat tattgacaac
    31981 cagcgtgggg caacgcttag tagttcaatt gatcctgcca atggtgttga tacgtctgaa
    32041 ttggcaaaat ttttcaatct tttttctgca gttgtatttc tatacagtgg tggaatggtc
    32101 tttattttag aatccataca attgtcttat aatatatgcc cgttattttc tcaatgttct
    32161 ttccgtgtct caaatatctt aacatttctg actttattgg caagtcaggc tgttatttta
    32221 gccagtcctg ttatgatagt attgttacta tcagaagtat tacttggtgt attatcgaga
    32281 tttgctccgc agatgaatgc tttttccgta tcattaacta ttaaaagttt acttgcaata
    32341 tttattattt tcatctgttc ttctactatt tacttttcta aagttcaatt tttcctcggt
    32401 gaacataaat ttttcacaaa tctatttgtt agataaaata ttatggcaaa taaaacagaa
    32461 aagccgacac ctaaaaaact aaaggatgcc gcaaaaaaag gacagtcatt taaatttaag
    32521 gatttaacga cagttgttat tattctggta gggacattta ctataatatc attcttttcc
    32581 ttaagtgatg taatgctctt atacagatat gtaataatta atgacttcga aattaatgag
    32641 ggtaaatact tttttgcagt ggttattgtc ttttttaaga taattggctt cccacttttt
    32701 ttctgtgttc tttcggctgt gttgccaaca ttggttcaaa caaagtttgt tcttgcgact
    32761 aaagctatca agattgattt ttcagtatta aaccctgtta aagggttaaa aaaaatattt
    32821 agtataaaga caataaaaga atttttcaaa agcattctgc ttcttattat tctagcatta
    32881 acaacctatt tcttttggat taatgaccga aaaataattt tttctcaggt gttttctagt
    32941 gttgatggct tatatcttat ttgggggggg ctatttaagg atataatatt atttttctta
    33001 gcattttcta tttttgttat tattcttgac tttgtgattg agttcatttt atacatgaaa
    33061 gatatgatga tggataaaca ggagataaaa agagaatata tagagcaaga gggacacttt
    33121 gagacaaagt cgagaaggcg tgagttgcat atcgagattc tttcagagca gactaaatct
    33181 gatatacgta attcaaaatt agtggtaatg aacccgactc atattgcaat tggtatttat
    33241 tttaatccag aaatagcgcc tgcacctttt atttctctca ttgaaactaa ccagtgtgcc
    33301 ttggctgtca gaaaatatgc aaatgaagtt ggtataccaa ctgtgcgtga tgtgaaatta
    33361 gctagaaaac tatataaaac acatacaaaa tatagttttg ttgattttga acacttggat
    33421 gaagtcctac gtcttattgt ttggcttgag caggttgaaa acactcatta ataagtaaag
    33481 gagatgtgtt atatgggagt taatttctgt aataaaatag gtattgatca gtctgaattt
    33541 gaaatagaat cttccatcat taactccatt gctaatgagg tattgaaccc aatatctttc
    33601 cttagcaaca aggatataat aaatgtttta ctcagaaaga tttcatctga atgtgacctg
    33661 gttagaaaag acatttatcg ttgtgctctt gagttggtcg ttgaaaaaac acctgatgat
    33721 ttataatcat tcataataac tcttttgttg atataaatat ttttgttgaa gatatttatg
    33781 tagtcacagg atcaattgta gaaaattgtt atgtttttgt taggaactga actaaataat
    33841 atatgataag acagcaaaaa cgactgacca taatccttct tttattagga gttgataaga
    33901 gagactattc atcctgtaat gttaaaactc tgttatatag tattcgcgat tatgcaaagt
    33961 ctgtaaatga tcatgagata ctaaccgaga gtaatcgttt attatctcat tgcatttcag
    34021 atagtaacgg agctttcttt aaaagtagta aatacgttcc ccttaagtac ttacgtaaaa
    34081 ggagaatagc acgaaagata ccaaatgatt agcactttgc tagtaagctt gctggtaaac
    34141 tatattgacc gttgggtttg ttttgagact gtaatttaaa ttgtgtaatt gcctgttttt
    34201 gatatgttta ctccaacaac ggagacaggt aaattatgga cgaaaagaag cttaaagccc
    34261 ttgcggctga actggctaaa ggcctcaaaa ctgaggccga cctcaatcag ttttcccgta
    34321 tgctgacgaa atatcccggc tcttaaaacg atagtggctc aggctttcat ttttcagcgt
    34381 tccccagaag atttccatcg gagcgttgtc gtaacagtta cctttacgcg acattgatgt
    34441 tttcagacca gactgctcct gtatgacccg gtaatcgtat gcgcagtact gtgaacctcg
    34501 atcggtgtgg tggattagcc cggcaggtgg gcgctggctc ctgagcgcca taaacagggc
    34561 tttacccgtc aattctttcg tcatgcgctc tcccatggcg taaccgacaa tttcgcacgt
    34621 ataaacatct ttgacgccgg cgagatacag ccatccttcc cgggtggcaa catacgttat
    34681 atctgcgacc cagacctgat taggtgctgt gggcgtaaag ttctgattca gcagattcgg
    34741 cgtaaccggc ggattgtggt cagagcttgt ggtcgccctg aacttgcgtt tctgcttaca
    34801 gtgcagcctc agctccttac gaagacgtgc cagtctgtca cgaccgacga t
//
LOCUS       DAJTSL010000027        34463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTSL010000027 DAJTSL010000000
VERSION     DAJTSL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.027"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            112..718
                     /locus_tag="OO433_001146"
                     /pseudo
     CDS             112..718
                     /locus_tag="OO433_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            821..1323
                     /locus_tag="OO433_001147"
                     /pseudo
     CDS             821..1323
                     /locus_tag="OO433_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1390..2847)
                     /gene="nuoN"
                     /locus_tag="OO433_001148"
     CDS             complement(1390..2847)
                     /gene="nuoN"
                     /locus_tag="OO433_001148"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6049694.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQSLISIVRLAMPLM"
     gene            complement(2854..4383)
                     /gene="nuoM"
                     /locus_tag="OO433_001149"
     CDS             complement(2854..4383)
                     /gene="nuoM"
                     /locus_tag="OO433_001149"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6049695.1"
                     /translation="MLLPWLILIPFIGGFLCWQTEHFDVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4547..6388)
                     /gene="nuoL"
                     /locus_tag="OO433_001150"
     CDS             complement(4547..6388)
                     /gene="nuoL"
                     /locus_tag="OO433_001150"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6049696.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYFCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQSVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWQGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6385..6687)
                     /gene="nuoK"
                     /locus_tag="OO433_001151"
     CDS             complement(6385..6687)
                     /gene="nuoK"
                     /locus_tag="OO433_001151"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6049697.1"
                     /translation="MIPLQHGLILSAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6684..7238)
                     /gene="nuoJ"
                     /locus_tag="OO433_001152"
     CDS             complement(6684..7238)
                     /gene="nuoJ"
                     /locus_tag="OO433_001152"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6049698.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7250..7792)
                     /gene="nuoI"
                     /locus_tag="OO433_001153"
     CDS             complement(7250..7792)
                     /gene="nuoI"
                     /locus_tag="OO433_001153"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6049699.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7807..8784)
                     /gene="nuoH"
                     /locus_tag="OO433_001154"
     CDS             complement(7807..8784)
                     /gene="nuoH"
                     /locus_tag="OO433_001154"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6049700.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8781..11507)
                     /gene="nuoG"
                     /locus_tag="OO433_001155"
     CDS             complement(8781..11507)
                     /gene="nuoG"
                     /locus_tag="OO433_001155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6049701.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11560..12897)
                     /gene="nuoF"
                     /locus_tag="OO433_001156"
     CDS             complement(11560..12897)
                     /gene="nuoF"
                     /locus_tag="OO433_001156"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6049702.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12894..13394)
                     /gene="nuoE"
                     /locus_tag="OO433_001157"
     CDS             complement(12894..13394)
                     /gene="nuoE"
                     /locus_tag="OO433_001157"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6049703.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13397..15199)
                     /gene="nuoC"
                     /locus_tag="OO433_001158"
     CDS             complement(13397..15199)
                     /gene="nuoC"
                     /locus_tag="OO433_001158"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6049704.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15293..15955)
                     /gene="nuoB"
                     /locus_tag="OO433_001159"
     CDS             complement(15293..15955)
                     /gene="nuoB"
                     /locus_tag="OO433_001159"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6049705.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15971..16414)
                     /gene="nuoA"
                     /locus_tag="OO433_001160"
     CDS             complement(15971..16414)
                     /gene="nuoA"
                     /locus_tag="OO433_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6049706.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17045..17983)
                     /gene="lrhA"
                     /locus_tag="OO433_001161"
     CDS             complement(17045..17983)
                     /gene="lrhA"
                     /locus_tag="OO433_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6049707.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18902..20119
                     /gene="alaA"
                     /locus_tag="OO433_001162"
     CDS             18902..20119
                     /gene="alaA"
                     /locus_tag="OO433_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6049708.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20203..20802
                     /gene="yfbR"
                     /locus_tag="OO433_001163"
     CDS             20203..20802
                     /gene="yfbR"
                     /locus_tag="OO433_001163"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6049709.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVLTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20861..22693)
                     /locus_tag="OO433_001164"
     CDS             complement(20861..22693)
                     /locus_tag="OO433_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6049710.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPVGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22780..23430)
                     /gene="hxpA"
                     /locus_tag="OO433_001165"
     CDS             complement(22780..23430)
                     /gene="hxpA"
                     /locus_tag="OO433_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6049711.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPKVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     AEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQLNGDVIIQ"
     gene            complement(23441..23935)
                     /locus_tag="OO433_001166"
     CDS             complement(23441..23935)
                     /locus_tag="OO433_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6049712.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACLRQYHLSANEINQ
                     IINA"
     gene            complement(24018..24473)
                     /locus_tag="OO433_001167"
     CDS             complement(24018..24473)
                     /locus_tag="OO433_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6049713.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24811..26013
                     /gene="ackA"
                     /locus_tag="OO433_001168"
     CDS             24811..26013
                     /gene="ackA"
                     /locus_tag="OO433_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6049714.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26088..28232
                     /gene="pta"
                     /locus_tag="OO433_001169"
     CDS             26088..28232
                     /gene="pta"
                     /locus_tag="OO433_001169"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6049715.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNQEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28422..29942
                     /gene="yfcC"
                     /locus_tag="OO433_001170"
     CDS             28422..29942
                     /gene="yfcC"
                     /locus_tag="OO433_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6049716.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVSRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29975..30517)
                     /gene="yfcD"
                     /locus_tag="OO433_001171"
     CDS             complement(29975..30517)
                     /gene="yfcD"
                     /locus_tag="OO433_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6049717.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30575..31126)
                     /gene="yfcE"
                     /locus_tag="OO433_001172"
     CDS             complement(30575..31126)
                     /gene="yfcE"
                     /locus_tag="OO433_001172"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6049718.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31182..31814)
                     /gene="yfcF"
                     /locus_tag="OO433_001173"
     CDS             complement(31182..31814)
                     /gene="yfcF"
                     /locus_tag="OO433_001173"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6049719.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASVAMAEHLLALGQPNLFGEW
                     CIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31950..32597
                     /gene="yfcG"
                     /locus_tag="OO433_001174"
     CDS             31950..32597
                     /gene="yfcG"
                     /locus_tag="OO433_001174"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6049720.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGELGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32654..33016
                     /gene="folX"
                     /locus_tag="OO433_001175"
     CDS             32654..33016
                     /gene="folX"
                     /locus_tag="OO433_001175"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6049721.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33037..33930
                     /locus_tag="OO433_001176"
     CDS             33037..33930
                     /locus_tag="OO433_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6049722.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIDSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33978..>34463)
                     /gene="rpnB"
                     /locus_tag="OO433_001177"
     CDS             complement(33978..>34463)
                     /gene="rpnB"
                     /locus_tag="OO433_001177"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6049723.1"
                     /translation="WLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELI
                     QKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEIAERAP
                     QEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQS
                     H"
BASE COUNT         8634 a         9378 c         8622 g         7829 t
ORIGIN      
        1 tgtatagatc taacaaccgt aataaaacaa atactcataa ctcattataa ataatgataa
       61 aataacgtct tttattgata atttatatat gggaaaaatt ccttttgtta tattgtttct
      121 gatttcactc cctctgatta tttttaccaa ccctgggtca ctgcattacc accaacgccc
      181 cgacacgtca gtcctgagca attagaaaaa acagttcgtt atcttacaca aactgtgcat
      241 ccacgtaatg ccgacaatat tgataatctg aataggtccg tagagtatat aaaagagttc
      301 tttgtcagta gcggtgccag agttaccgcg cagggcgtcc ccgattggct attacgactc
      361 tacgcctggt agccaggatt acctttaccc gcaatgtcct ggctttatcc cgatcggggg
      421 gactttattg cctggtcggc agaatacagg atatcaacgc cgttcgtcag gtaaaaacgg
      481 cattgttatc atcccgggat ttatctgttt attctatgaa tgccccatgg tttattcccg
      541 gtattgattt ctctgaccac ctgaattatt ggcaacacga tattcccgcc gtaatgatta
      601 ctgacaccgc tttttatcgt aataaacaat accacttgcc cggtgataca gcagacagat
      661 tgaattatca gaaaatggct cagatggtgt tataaccttg ttacacaaca gtaaataata
      721 aaaatttctc gaggcgggtc cagaaacagc gacatgtaca ttttattttc cctcgtctgt
      781 tttaagatgc gaaacataaa attcaatgga ggaaaaataa atgggaatga ttggttattt
      841 tgcagaaatt gactcagaaa agattaacca atctgcttga gtctactgaa aaacctttga
      901 tggataatat acatgacacg ctttcaggat tacgccgact ggatattgat aaacgctggg
      961 attttttaca ctttgggctg acaggcaccc ctgcttttga tcccgcgaag aatgatccgc
     1021 tgagtcgtgc cgtgcttggt gaacacagcc tcgaagatgg catagatggc ttcctgggat
     1081 tgacctggaa tcaggaatta gccgcgacaa tcgaccgact ggaatcactg gatcgaagta
     1141 agctgcgtaa acagttttcc attaagcgac tatgaaatgg agatttatcc gggagtgacc
     1201 ttttcagaag agttagaggg gcaactcttt gcctcgatca tgcttgatat ggaaaagctg
     1261 attagcgcgt atcgacgtat gctgcgacaa gggaatcatg cgctgacggt tattgttggg
     1321 tgatggtgtc agagcgccaa aaaaaaccgc cgaacatgtc ggcggttttc tgacttacaa
     1381 actaacagat tacatcagcg gcattgctaa ccgcacaata ctaatcagcg attgtggcca
     1441 tacacccagc accagtacca acagtgcaga aatcagcacc acgataccgc ccgcgctgta
     1501 ctgccagttt gacggtgcat cgcgacccgg ttgttccggt gcgtgcaggt acaggctcac
     1561 cgccacgcgc aggtagtagt agaggccgat tgccgaaccg acaaccacgg cacccaccag
     1621 ccaccacaag tgtgcctgga caccgaccgc cagcacgtag aacttaccga taaagcccag
     1681 cgtcatcggg ataccggcca gagacagcat catcaccgtc atcactgccg cgaggatcgg
     1741 acgatgccag aacagaccgc ggtaagagaa cagggaatca gcatccgggc cacgatacgg
     1801 gctggacatc aggctgacca cgccgaacgc gccgaggctg ctgaacagat aaccggccag
     1861 gtaaacccct accgcttcca tcgacatctc gccggtttgc agcgcaatca gcgccaccag
     1921 cagatagccg aggtgagaga tagatgagta accgagcaga cgtttgatgt tggtctggct
     1981 caacgccatc aggttaccga agatgatgga ggcaaaggcg ataatcgcca gtactacgcg
     2041 aatcgcttcg ctgtcaccca ccggtgcata gaggaacaga cgcatcacca caccgaagat
     2101 agcgattttg ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc cttggtatac
     2161 gtctggcgtc cacaggtgga acggcaccag agagagtttg aagccgaggc caacaatcat
     2221 catgccgaaa cctgccagca acagcggctc gttgagcata ccgtcaccga ggtttttacc
     2281 cagcgcgaca aacgacaggt cgccagactg cgcatacacc agcgccatac caaacagcag
     2341 gaaagaagac gctgcggcag aaaggatggt gtatttgata ctggcttcca gcgaacgttt
     2401 ctggcggaaa gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt caataccgag
     2461 gaacagagac gccagatggt ttgcattcgc cagcaggatc ccgcccagcg cggcaattaa
     2521 caccaacagg tagaactcat ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa
     2581 agtacaggtg gcgaggctcg ccaacaatac cagcccggtg taaagcatgg cgaaaccatc
     2641 aacgcgcatc agcggcgtaa cgtccatagc gcccgcctgg ccaacaaacc agagcgaaac
     2701 cagcgccgcg ttaagcccaa taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca
     2761 cgcaatggag agcatcacaa ccaccaccgt taagccgacg atcagcaacg gtagcagtgc
     2821 gatcaggttt tgtggagtta ttgtcatggc gatttacggc cttgtagtag taacggaatt
     2881 aacaaaccac tgctggatat tgccaatcgc ggagtgcgag gtatccagaa tcggctgcgg
     2941 atagaagccc agcagtacca gcagcaccac cagcaacagg atcataaaca gctcacgcag
     3001 cgacatccct ggcagttcct ggctggcaat ctggcttttc gctttaccga agtaagcgcg
     3061 atgcaacatc gccagcgaat aaacagatgc aaagaccagc ccaaaggtag agataacggt
     3121 aatcaccggg acaacctgga agctgccgaa cagaatcata aattcgccga cgaagttacc
     3181 ggtgccaggc atcccaagcg ttgccaccgc aaagaacagc gacaatgctg gcagccattt
     3241 catcttgctc cacagaccgc ccatcatgcg catgtcgcgg gtatggatac gttcataaag
     3301 ctgaccacaa agaataaaca gacccgccgc cgacaaaccg tgcgcaatca tctggattac
     3361 cgcgccctgg taggccaact ggctgccggt gtagatagca atcagcacga agcccatgtg
     3421 ggaaaccgag gtgtaggcga tcagacgttt gatatcggtc tgggcgaagg ccatccacgc
     3481 accgtagaag atgccgataa cacccagcca catagcaatt ggcgcgaact ctgccgacgc
     3541 gttcgggaac agcggcaggg agaaacgcag caaaccgtaa gcggcagttt tcagcaagat
     3601 ccccgcgaga tcaacggaac cggcggtcgg agcctgggag tgcgcatccg gcagccagcc
     3661 atgcagcgga accaccggca ttttgactgc gaaggcgatg aagaagccca gcattaacag
     3721 gtattccaca ccactggaca ttggcgtatt cagcagctct tcatagttga aggtccagac
     3781 gccggtcgca ttgtagtgaa caaaaaccag cgccaggatg gcaatcaaca tcaccaggcc
     3841 actcgcctgg gtgtaaatga agaacttggt tgccgccgtg atacgcgttt taccgtcaga
     3901 ggctttatgc ccccacagtg cgatcaggaa gtacatcggc accagcatca tttcccagaa
     3961 gaagaagaac aggaacatgt cgatggcaag gaacacgccg ataacgccgc ccaggatcca
     4021 catcaggttg aggtggaaga agccctgata tttttcgatc tctttccacg aacagagtac
     4081 cgccagcaca ccgagcagac cggtcagcac gaccatcagc agcgacagac cgtcaatggc
     4141 gagatgaata gagataccaa aacgcgggat ccacggcatg tcgaattcag actgccactg
     4201 cggaattccg gcggattgcg tcagtgaata accgccctgc aaccacagtt gcagcgacag
     4261 cgccagcgtc aatcccatgg tgatcagcgc gatccagcgt ggcaccttga cgtcaaagtg
     4321 ttcggtctgc cagcacagga agccgccaat aaagggaatt aatattagcc agggtagtaa
     4381 catggcgatc tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa cgaattctca
     4441 cgacagaaaa ttctcttcgg actgggggta gtgtcggatg cgccgcgaag gcagcttatc
     4501 cgacctacgt tcttatatgc ccaggagcaa tcccacaatc cttaactcaa cgcagtacca
     4561 tcaacagtgc cagcaccacg accgcaccga tgctcatgga tgccacatac cagcgcagat
     4621 aaccgttctc gcttaacagc aggcctttac ctgcaaagcg ggaaagaacc gccggaatgt
     4681 tcatcattga gttcagcgga tcgcgtttca gcaaccaggc aatacccaga aacggcttga
     4741 cgaacacttt gtcgtacagc cagtcaaagc cccaggcgtt gtaccaccag gtgcccagca
     4801 gacggcccgg cgcactgttg gcaatggagg tcaccagagt acgtttaccc tgccacagcc
     4861 aggctgccag cagaatgccg accaccgcga ccacgccaga ggtaatttcc agggtcaaca
     4921 tgctgccgtg cgccagttcc gtcgtttgcg gaagcacgct ctgcagcggc ggtacaatca
     4981 gtgcgccaac gaaggtggaa aggatcagca gcacaatcag cggcaggctg tgagttaccc
     5041 ctttcacggc gtgagcgtga atttgttctt ttccgtggaa gacgatgaaa atcatacgga
     5101 aggtgtagag cgaggtcata aacgcaccga ccagacctgc caccatcaga ttgatatgac
     5161 cattcgccat cgcacccgcg aggatctcat ccttactgaa gaagcccgca gtgaccagcg
     5221 gtagtgccga cagtgctgcg ccgcccacca ggaagcagaa ataaaccagc ggaatagatt
     5281 tacgcagacc gcccatcttg aagatgttct gttcgtgatg gcaggccaga atgacggaac
     5341 cggatgccag gaacagcagc gctttaaaga acgcgtgagt catcaagtgg aaaatcgccg
     5401 catcccatgc ctgcacgcca agcgcgagga acatgtagcc aatctggctc atggtagagt
     5461 aagcgagaac acgtttgatg tcggtctgta ccagcgcggc aaaaccggcc agcagcagcg
     5521 taaccgcccc gacaatgccc accagatgca gaatttccgg cgtcatcagg aacaggccgt
     5581 gggtacgggc gatcaggtag acacccgcgg ttaccatggt tgcggcgtgg atcagcgcgg
     5641 agacaggcgt cgggcccgcc atcgcgtcgg caagccatgt ctgcaacggc aactgcgcag
     5701 atttaccgac cgcaccgccc agcagcatca gcgtcgccca catcagcatg ttattgccgt
     5761 cagcaaagtg cgctggtgcc agttccacca tttcgcggaa gttcagggtg cccagttcgt
     5821 tgtaaagaat gaacagcgcg aaagcgagga acacgtcacc cacacgggtc acgacgaacg
     5881 ctttcattgc cgctgcgcca ttcttcggat cggtgtaata gaacccgatc agcagatagg
     5941 agcacaggcc cacgccttcc cagccgaggt acatcagcag caggttatcg gcgagaacca
     6001 gaactaccat gctggcgatg aacaggttgg tgtaggcgaa gaagcgagag tagccctctt
     6061 caccgcgcat gtaccaggag gcgtacatgt gaataaggaa acccacacca gtgaccaccg
     6121 agagcatggt cagcgacagg ccgtccagca ccaggttaaa accgatgtta aagtcgccta
     6181 ccgacatcca cgtccacagc ggctggctgt atgcctgctc gccgttagcg aagaaatcaa
     6241 cgccgataaa ggcggttacc agcgccgcca ggcccacaga gccaacgccg acgatcgccg
     6301 aaacgttttc agaccagcgc ccacgggaga acgccagcag gacgaagcca atcaatggca
     6361 aaataatggt taaggcaagc atgttcatcc gcgcatctca cttactgaat cgatgttcag
     6421 gttctggcga cgacggtgaa gttgcagcag cagcgcaagg ccgatactcg cttctgccgc
     6481 cgcgaggctg atggcgagaa tgtacatcac ctgaccgtcg gtctgacccc agtagcttcc
     6541 ggcgaccaca aaggccagcg cggaggcgtt aatcatgatt tccagaccaa tcagcataaa
     6601 cagcagattg cgacggataa ccagaccggt taagccaaga acgaataaga ttgccgagag
     6661 gatcagtcca tgttgtaagg ggatcatgcg tgctcctccg tttttctttt cgcgctgtcg
     6721 tctttacgat tgctcagcac ttcacccgca cgctcttcac gaccgacgtg gaaggccaca
     6781 accagacccg cgagcagcag catagaagcc agttccaccg ccagtacgta aggcccgaac
     6841 agcgtaatac ccactgcttt agcactgatt ggcgtaccgt cgataccctg atcattaaca
     6901 ccaaggatgg cgtaaacaat caccaccagc atgatggccg acaaaattgc cggaccaatc
     6961 cacacctgcg gtttcagcca ctggcgttcc tgttcgattt ctgaaccgcc caggttgagc
     7021 atcatcacca cgaacacgaa cagcaccata atggcacccg cgtagacgat aatttccagc
     7081 gcaccggcga agtaagcgcc cagtgagaag aacaccccgg aaatcgccag cagcgaaata
     7141 atcaggtaca gcagtgcgtg taccggattg gtatgggtga tcactcgcaa ggtcgcaagt
     7201 atggctatca ggccacagat ataaaaagcg aactccattg cccctctcct tacggtaaca
     7261 ggctcttgac gtcgataggc ttggcttcgt tctctgcttc gcccttatct ttgccgtcga
     7321 ttgccatacc tgccatccgg tagaagttat attccgggta tttgcccgga ccggagatca
     7381 gcagatcctc tttttcgtaa accagatcct ggcgcttgta ttcccccatt tcgaaatccg
     7441 gggttaactg aatcgccgtg gtcggacagg cttcttcgca cagaccacag aaaatgcagc
     7501 gtgagaagtt gatgcggaaa aattccgggt accagcgacc gtctttggtt tctgcttttt
     7561 gcagcgagat acagccgacc gggcaggcta ccgcgcagag gttacaggct acgcaacgct
     7621 cttcgccgtc cgggtcgcgg gtcagaacga tacgaccacg ataacggggc ggcagataga
     7681 ccggctcttc cgggtacatt cgcgtttcgc gtttggcgaa cgcgtgcagg ccgatcatcc
     7741 agatactacg aacctgggtg ccgaaaccta ctaacaattc ttttaaggtc atggtcttat
     7801 tgccccttat tgcgcctgcc agagaatgac agccgccgtt accagcaagt tgatcagcgt
     7861 cagcggcagg cagattttcc agccgaagga cattacctgg tcataacgcg gacgcggtaa
     7921 cgacgcacga atcaaaatga acatcatcat aaagaacgcg gttttcaacg cgaaccagat
     7981 gaatggcggt aacaacgggc cttgccagcc accgaagaac agcgtcacca tcaatgcaga
     8041 gatggtcaca atcccgatgt actcacccac gaagaacaga ccgaacttca taccggaata
     8101 ttcaatgtgg taaccatccg ccagttcctg ctcggcttcc ggctggtcaa acgggtgacg
     8161 gtgacatacc gccacgcccg cgatggcaaa ggtaataaaa ccaaagaatt gcgggataac
     8221 gttccacaca tgcgcctggc tgttgacgat gtcggtcatg ttgaatgaac cggcctgcgc
     8281 caccacgccc atcagggaaa gcccgaggaa cacttcgtag ctcagggtct gcgcagaagc
     8341 acgcatcgca cccagcaacg agtatttgtt gttacttgac cagcccgcaa acagcaccgc
     8401 gtaaaccgcc agacctgcca tcatcaggaa gaacaaaatc ccgatgttca ggtcggcaac
     8461 cacccaaccc ggactgactg gcacaatcgc aaaggccagc agcagcgagg taaaggcaat
     8521 catcggtgcc agggtaaaga tgacgcgatc cgagaatttc gggatccagt cttctttaaa
     8581 gaacattttg atcatgtccg caaccagctg gagcgaaccg ccccagccaa cacggttagg
     8641 tccataacgg ttctggaaca gacccagcag gcgacgttcg ccaaagctca tgaatgcccc
     8701 acaggtgaca accaccagca ggatcaccac cgctttgagg atggtcagca ggatctcaat
     8761 cagttccggt gatatccaac tcattgttgt gcctccttga gatcctcaag atgcgcgcca
     8821 gccagcaccg gagcaatgcc ggacataccc atcggcaagc ccacctgccc tgccgtcagt
     8881 ccttcggcga tttcaaccgg cagcgtgacc gtgttgccat cgtaactaaa ggagacgcgt
     8941 gtacctgcgt tcacacccaa cttcgcggca tccgctgggt tgagtttgat gtacggctgc
     9001 ggcatacggc tctggaagac cggagcacgc tgtgacaatt catcgctgcc aaacaggtgg
     9061 taatacggcg caatacgcca tttcccgtcc tgcggctgga agcgtgccgg tacgctggtg
     9121 aagtaatcca gaccattctc gctggtttca aacagacgca cgcccggatc gccaaagcgc
     9181 agtttgccgc ccacttcgtc ctggaatttg ttccacgcct gcggggagtt ccagcccggt
     9241 gcccaggcaa acggcacttg cgaacggtgc gcggtcggct ggttgttacc ttccatcgag
     9301 aaggtgaaca tggtgtcaat atcctgcggc tgacgcggct catgaacgct gatattggcg
     9361 cgcatggcgg tacgaccgct gtaacggtgc ggttcacggg ccagtttctg cccacgaata
     9421 cggaatgtcg catccggcgc agcatctttg atacctgcca gttccgggat tttcgccaca
     9481 acagcgtcaa tcacatggtc gagctgcgtc cagtccactt cacggctcag cagggtgctg
     9541 tgcagcgagt gcagccagcg ccagctttcc agcatgacag ttttgctgtc gtaataagcc
     9601 ggatcgtaaa cctggaagaa acgttgggcg cgaccttcat tattaattac cgtaccgtcg
     9661 ctttcagcaa agctggcggc ggaaagtacc agatgggcgt tttccatgat cgctgtacgc
     9721 tgatggtcaa ccaccatcac cagcggtgct ttagccagcg cagcattcac gcgggtagca
     9781 gaagcgtgac gatgcagatc gttttccaac accaccaccg cgtcggcgcg tccggtttcc
     9841 agttcggtta acgcttcttc aagcgaaccg ccacccataa tgcccagccc catgctgttg
     9901 acggaacggg caatcatggt gataccgacg tcagcgccgc gacctttcag ggctttcgcg
     9961 acgttagccg ccgcctgaat cacctctaag ctaccggcgt tcgtcccgga gataatcaac
    10021 ggtttcttcg cacctgccag tgcctgcacg atgacgtcga ttttgctttg cagctcaggt
    10081 tcgataccgt caaccgctgg tgcagagtta tccagcgcat gggcgatggc aaaacctaaa
    10141 cgcgcctgat cttcaaccgg tgcgcggtaa gtccacgccg cgatatcatc cagacgggtg
    10201 tcatcaacgt tggtaacaaa cagcggatgc ttcgcacgtt gaccgatgtt aaggattgcc
    10261 gcaatctgcc agtcagccac tttctgtgct gccgccattt cgcgcgcttt acctttcacc
    10321 gcctgacgca ctgccagcgc gacgcgcgcg ccggtctggg taacgtcttc acccagcacc
    10381 agtaccgcat cgtaagattc gatttcgcgc agagccggag tataaatgcc gccttcacgc
    10441 agcactttca gcgccagttg cagacgttcc tgctcaccgt gagcgatacc ggtgtagaag
    10501 ttttcttcgc ccaccagttc acgcagtgca aagttgcttt ccacgctggc acgcggagaa
    10561 ccaataccga tcactttctt cgactgacgc agaatatctg ccgcgccctg cattgcctgt
    10621 tcggcgttga gggtaatgaa atcatcgcca cgacgctgta ctggctgacg cggacgatcc
    10681 ttcaggttga cgtaaccgta accgaaacga ccacggtcgc agaggaagta gtggtttacc
    10741 gtaccgttgt aacggttttc gatacgacgc agttcgccgt aacgttcacc ggggctgatg
    10801 ttacagccga tggaacattg ctggcagatg ctcggcgcaa actgcatatc ccatttacgg
    10861 ttgtaacgct cggagtgcgt tttgtcggta aatacgccgg tcgggcaaat ttcgaccagg
    10921 ttaccggaaa attcgctttc cagcgtgccg tcttccgggc gaccgaagta gacgttgtcg
    10981 tgcgcgccat aaacgcccag atctgtaccg tcagcgtaat ctttgtagta acgcacacag
    11041 cggtagcagg cgatgcagcg gttcatttcg tgagatatga atggccccaa atcctgatta
    11101 cggtgggtac gtttggtgaa acggtagcga cggaagctgt gtccggtcat cacggtcata
    11161 tcctgaagat ggcagttacc gccttcttca cataccggac agtcgtgcgg gtggttggtc
    11221 attaaccact cgaccacact ttcacggaac tgtttcgctt cttcgtcgtc aatggaaata
    11281 aaggtgccat cggaagccgg tgtcatacag gacatcacca ggcgaccacg cgtgtcttcc
    11341 gcgttttggt attgcttcac cgcacactgg cggcaagcac cgacgcttcc cagcgccgga
    11401 tgccagcaaa agtaaggaat atcaaggccc agagacagac aagcttccag caggttgtcc
    11461 gctccgttga cctcgtattc tttgccgtct acatgaattg tagccattag catgcttcca
    11521 gttttctcgg tcagggaccg agcgttaatc gaaattcggt taccagcgct ctttcagcag
    11581 gttcggctga atcccattaa tcaaatgggt attgctgaac ggctgtttga ttcccgcctc
    11641 aaattcttcg cggaaatatt tgatggcgct ctgtaacggc tccactgcac caggtgcgtg
    11701 ggcacagaaa gttttacccg ggcctaagaa tcgacacagt tgctcaagtg tttcaatatc
    11761 gcccggctgg ccttcgccac gctccagcgc acgcagaatt ttcacgctcc acggcagacc
    11821 gtcgcggcac ggcgtacacc agccgcagga ctcacgggcg aaaaactctt ccaggttacg
    11881 caccagcgac accatgttga tctcatggtc aaccgccatc gccagcgccg tacccagacg
    11941 gctgcccgct ttaccgatac tttcgaattc catcggcaga tcaaggtgcg cttcggtcag
    12001 gaagtcagtc cccgcgccgc ctggctgcca ggctttaaat ttcagaccat cacgcatacc
    12061 accggcgtaa tcttcgagga tctcgcgtgc ggtggtgccg aacggcagtt cccacagtcc
    12121 cggatttttc acccgaccgg agaagcccat cagcttggtg ccagcatctt tacttttcga
    12181 gatgttctga taccactcca cgccgttagc gaggatcgcc ggaacgttac acagggtttc
    12241 gacgttgttg acacaggtcg gtttgcccca tacgccggag gttgccggga aggggggttt
    12301 cgagcgtggg ttagcacgac gcccttctag ggagttgatt aacgctgttt cttccccgca
    12361 gatgtagcgc cctgccccgg tatggacgaa cagttcgaag tcgaaacctg ttcccataat
    12421 gtttttgcca agcaagcccg cttcagtggc ttcggcaatg gcgcggcgca gatttactgc
    12481 cgcttcgata tattcgccac gcaggaagat gtagccacgg taagctttca gcgcaaacgc
    12541 ggagatgagc ataccttcca ccagcaggtg cggcagttgc tccatcaaca ggcggtcttt
    12601 ataggtgccc ggctccattt catcggcgtt acacagcagg taacggatgt tcatggattc
    12661 gtctttcggc atcaggctcc acttcaggcc agtagaaaag cccgctccgc cgcgcccttt
    12721 cagaccagcg tcttttacct gattaacgat ttcgtccgga gacagcccgg tcagcgcctt
    12781 acgcgcccct tcgtaaccgt ttttgctgcg gtattcgtcc agccacactg gctgtttgtc
    12841 atcgcgcaga cgccaggtca gcggatgcgt ttcgggagta cggataatgt ttttcattta
    12901 taccgctcca gcagttcagg gatcgcttcc ggggtcagat gcgcgtgagt gtcctcatcg
    12961 atcatcatgt ttggcccttt atcacagttc cccaggcagc aagttggcag cagcgtaaag
    13021 cggccatcaa atgtcgtttg ccctggtttg atgttcagct ttttctcgag cgccgcctga
    13081 ataccctgat acccgttgat atgacagacc acgctgtcgc aataacggat cacatggcga
    13141 ccaaccggct ggcggaagat ctgactgtag aacgttgcca caccttcgac gtcgcttgcc
    13201 ggaataccca gcacatcggc gatcgcgtgg atcgcaccat ccggcaccca gccacgctgc
    13261 ttctgaacga ttttcagcgc ttcaatggac gccgcacgcg ggtcttcgta gtggtgcatc
    13321 tcgtgctcaa tcgcttcacg ctctgccgca ctcagctcaa aagcctcggt ttgtggttgt
    13381 tgattctcgt gcataattag cggtccacat ctgacataac aaaatcgata ctgcccagat
    13441 aaacaatcag gtcagacacc aggctgccgc ggatcgccgc cggaatttgc tgcaaatgcg
    13501 cataactcgg cgtgcggata cgagtgcgat aactcatggt gctaccgtcg ctggtcaggt
    13561 agtaactgtt gatccccttg gtcgcctcaa tcatctggaa agattcattg gcaggcatca
    13621 ccggccccca cgacacttgc aggaagtggg tgatcagggt ttcgatatgt tgcagcgtgc
    13681 gctctttcgg cggcggcgtg gtcagcgggt gatccgcttt gaacgggcct tccggcatgt
    13741 tgttgaggca ctgctcaaga atgcgcagac tctggcgcag ctcttccact ttcagcatta
    13801 cgcgggtgta gcagtcagaa acaccaccac caaccgggat ttcaaagtcg aagttttcat
    13861 agccagaata aggacgcgcc ttacgcacgt cgaagtcgat cccggtagca cgcaggcccg
    13921 cgccagtggt gccccactcc agcgcctctt tcgcgccata ggcggcaacg ccctgggaac
    13981 gacctttcag aatggtattt tgcagcgccg ctttctcgta agacgccaga cgtttcggca
    14041 tccagtcgag gaactcacgc agcaggcgat cccagccgcg cggcaggtcg tgcgctacgc
    14101 cgccaatacg gaaccacgcc gggtgcatac ggaaacccgt gatcgcttcc accagatcgt
    14161 aaattttctg acgatcggta aaggcgaaga acacgggcgt cattgcgccg acgtcctgaa
    14221 taaaggtcga gatgtacagc aggtggctgt tgatacggaa cagttcggag agcataacgc
    14281 gaatgacgtt aacgcgatcc ggcacggtga tcccggccag tttctctacc gccagcacgt
    14341 aaggcatttc gttaacgcag ccgccgaggt attcaatacg gtcggtatac ggaatgtagc
    14401 tgtgccagga ctggcgctcg cccattttct cagcaccacg gtggtggtag ccgatgtctg
    14461 gtacgcagtc gacaatctct tcgccgtcga gttgtaaaac gatacggaaa gccccgtgcg
    14521 ccgacgggtg gttcggaccg aggttgagga acatgaagtc ctcgttttcg gtgccgcgct
    14581 tcatccccca ctcttccggt ttgaaggtca gggcttccat ctccagatcc tgtttggctt
    14641 tggtcagctc aaacggcgag aattcggtag cgcgcgccgg gtaatcttta cgcagcgggt
    14701 gacctttcca ggtttgcggc atcatgatgc ggcgcaggtt cgggtgaccg tcgaaagtaa
    14761 tgccaaacag atcccaggtt tcacgctcat accagttagc gttcgggaac agtttggtga
    14821 aggtcggtac gtgcaggtcg ttttctgcca gcgccacctt cagcatgatg tcgcggttac
    14881 gatcgataga aatcagatgg tagaaaacgg aaaaatccgc ggcaggtaac ccttcgcggt
    14941 gtgtgcgcag acgttcgtcc atgccgtgta agtcaaacag catgacgtaa ggtttcggca
    15001 gtttctttaa gaaatcgcca acttccagta attgttcacg cttgatccac acaacgggaa
    15061 ccccggtgcg agtcgcctga acagtaaagg catccggccc aaaacggttg cgcagttcgc
    15121 caattaccgg atcatcaaga tgatcgcggg tctgccaggc gggttcttgc gcggttaagt
    15181 cggtcatatt gttcaccatt gcaaatggtc cgtggtgact gtcggcgcag taagcttcgc
    15241 gaaatagatt taggaataag cgaagaaaaa tccctttgcc gacaggcgca aattaaatct
    15301 cgtcaggtgt acgcaggttg gttacggcaa tgcgttcacc gcgcttgcgt tcgcgctctg
    15361 attgcatatt ggcgcgataa acgccctgat cgccaaccac ccaggagagc ggacgacgtt
    15421 ctttgccgat agattcctgc aacagcatca gtgcctgcat gtacgcttca ggacgcggcg
    15481 ggcagcccgg gatatacaca tcaaccggga tgaatttatc gacgccctgc acaacggaat
    15541 aaatatcgta cataccacca gagttggcac aggcacccat tgagataacc cattttggtt
    15601 ccagcatctg gtcatacaga cgctgaataa ccggtgccat tttggtaaag caggttcctg
    15661 caaccaccat caggtcagcc tgacgcggcg aagcacgcaa tacttctgcg ccaaaacgcg
    15721 ccacgtcatg caccgcggta aacgaagtca ccatctcaac gtaacagcag gaaagaccga
    15781 agttatacgg ccaaattgag tttttacgac cccagttaac catgtcattg agcttgccca
    15841 taaacacgtt tttgttaact tcttgctcca gagggtcggt tacgatctcc tgcttttgca
    15901 gggggtaacg gtcgttctca ccgttgggat ctatgcgggt gagcgtataa tccatcttaa
    15961 tgcctcgcgg ttagcgttga cgattagcga tactgttcgt ttccgggttc atacgctcgc
    16021 ggcgtgaacg cgcgggcgtc cagtccagcg cgccaatacg caccagataa accagacctg
    16081 ccagtaacac aaaaataaaa attgcagctt ccacaaagcc tacccagccg ctttcgcgga
    16141 tagaggttga ccatgcgaac agatacagcg cttcaacgtc gaagataacg aagaacatgg
    16201 ccaccagata aaacttggcg gacaggcgta agcgggcgga gccgaccgag tcgataccgg
    16261 attcaaacgg cacgtttttc gacctcgcgc gtgcgcgacc gcctaaaaac caaccaccta
    16321 ccagcattag gcaacacagg ccaatggcaa cgataagaaa gatagcgaat gcccagtgat
    16381 gagcgatgac ttcagtggat gttgacatac tcattgctta ctcatcaaaa gtagcgccag
    16441 attcactgct cttcacggca gatggacgcc acatcgattc atggggagga ataaaaaaaa
    16501 ccttacaatc actgtagaaa ttcttttata cagctaattg atgtggtctt ttactccttt
    16561 ctataacctt ttgtcaactt taacaaaagt ttcttcacat tagtttacat aatatcaaca
    16621 ccattagcat ttaatgccct ttcaccccag ctccttgacg actccaggat aattagatgt
    16681 tgttgaatcg tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt gacaggaatt
    16741 tgtgtcgatt cctcccccca aaagagagta ttttcttgat ctgtgacacg cttttgtcat
    16801 tccataacaa aaacgcagca acaaatttac gtatttttta acatcattgt agcaggtgat
    16861 ttttttcagg cgattatttg tgcgttcggg acgtgaatct ctggtgatta aaaaatgaac
    16921 agttttgtac gttctgcact atgcgaggaa ggatttttac taaaaaaaag ccgctggggt
    16981 ttaaaacacc cccagcggct cgttttttac actattgtct caggaattat ctatcgtccg
    17041 tcgattactc gatatccctt tcaatcaaca atgaatcatc cccttccgga gcagacattg
    17101 ggctgtattg ccacggatta tggtagctct ccatcgcctg atagatcacc tgggccagct
    17161 cattattact ggacgggtca tagcacagca aatattcggt atcaggtagc ggcggtaagc
    17221 catctactcc actcagcaca cgcaggtccg ggctcatcat ctcaaccggc cttgccgtca
    17281 cgccaagacc ggctttcact gccgcacgaa cggccggaag cgtcgaggcg acataagcca
    17341 gtcgccatgg aatatctgct ttattaagcg ttgccagcac catatcgcga aacggacttg
    17401 gatcatccag caatacaaga gggatcggct cgcctttttg caatacgtat tccgctgcgc
    17461 agtaccagtg tgttggcgag gtacgcaggt tcaatgcctt aaacgctgaa ggacgatggg
    17521 tggttaccat caaatccact tcctgggatt ccagcatttc tgccatatag gcattacgtt
    17581 taacgcggac atccagcgct aatttcggat aaaccgaact cacgcgattt aacaggaaag
    17641 gtaagatcgt atcggcagat tcatctgaag caccgatagt taacacgccc tgaagattac
    17701 tgaacattaa tgatgagcag gcctcatcat taaaacgcag gattttcctg gcgtaaccaa
    17761 gaagttgaat gccatgttca gttaacagtt tgttgcgacc gtgacgagcg aacagttctt
    17821 tcccaacgag ttgttccaga cgctgcattt gctgacttac ggcggactga gtacgacaca
    17881 cagcggcagc tgcggcagca aaagtgttca gatcggcaac agcaacaaat gttctcagca
    17941 gatcgaggtc gaggttaatt atcggacgat ttgcacttat catatattat cacttactgg
    18001 cggctcatac tgagctggtt aatgctgtgc acacacaaac aagcaattcc atttgtaatg
    18061 tgcctccctg gcagtttcat ccggaacccg gacgaaagta aaaatgcata tgagttgcac
    18121 taaaaaagcg actcacattg ttccgttata atgcctgaag tagatcacag aatatatctt
    18181 cagggatcgc atatctatta agttactcac tcttttctat ttatgacatg cgcgtgtttg
    18241 tataaatgta aatgtgagtc cttgttccac tctcgtgcag catcgctggt catacgcgaa
    18301 cacgtacaaa cagcaatggt gaggcggcat caagagcagg gatccgtcat ttatgcgagc
    18361 attttacccc aaaaaccttt tatttataag ggccattgcg aatcatctga tgcaaagtta
    18421 tgttatgtta ggcaaagtaa tcttctatta ttaataagca catcaaaacc tttttgaata
    18481 ttaaataata attaattagc atcatcctca ttcattaatt ccgcttaaca atagtttcac
    18541 accttgcccc attacgacac ctcggaaatc atcacgctgt taaactctcc gtaatgcgga
    18601 aatatcatca tcgcgtttca ttagtgaatt cttaaatgag gcattttcac acaattatct
    18661 tacagataaa aaaaccagac ttacaattaa gaatcagaac aattcactat ataacattgc
    18721 atgtaaagca tatacacctc attattttgt catcattaag ttattaacag cacaatcgag
    18781 ccttcccctc tggcaaaatc ttattctgca gaccttcaaa acaccgtcct gggggagtac
    18841 attgttctaa gctgacttcc acggcaggga gtggcgataa cagcaaaaaa ggtcaagatt
    18901 catgtccccc attgaaaaat ccagcaaatt agagaatgtc tgttatgaca tccgtggtcc
    18961 ggtgctgaaa gaagcaaaac gcctggaaga agaaggtaac aaggtactga aactgaacat
    19021 cggcaaccca gccccgttcg gttttgacgc gccagatgaa atcctcgttg acgtgatacg
    19081 caacctgcct accgctcaag ggtattgcga ttccaaaggt ctttactccg cgcgtaaagc
    19141 catcatgcag cactaccagg ctcgtggcat gcgtgatgtt accgtggaag atatttacat
    19201 cggcaatggt gtatcggagc ttatcgttca ggcaatgcag gcattgctga acagcgggga
    19261 cgaaatgttg gttcctgcac cagattaccc actatggacc gcggcggttt cgctttccag
    19321 cggtaaagcg gtgcattatc tttgcgatga atcctctgac tggttcccgg acctcgatga
    19381 tattcgcgct aaaattacgc ctcgtacgcg tgggatcgtt attatcaacc caaataaccc
    19441 aaccggcgcg gtatattcca aagagctttt aatggagatt gtggagattg cacgtcagca
    19501 taatctcatt atcttcgccg atgaaattta tgacaaaatt ctttacgacg acgctgagca
    19561 tcactcaatt gcgccgctgg cacctgacct gctgaccatt acctttaacg gactgtcgaa
    19621 aacgtaccgc gttgcaggct tccgtcaggg gtggatggtg ttgaacgggc cgaaaaaaca
    19681 cgccaaaggc tacatcgaag gtctggaaat gctggcttca atgcgcctgt gtgctaacgt
    19741 tcctgcgcaa cacgccattc agaccgcgct gggtggttat cagagcatca gtgaatttat
    19801 tacccctggc ggtcgtcttt atgagcagcg taaccgcgcg tgggaactga tcaacgatat
    19861 tccgggcgtt tcctgcgtga aacctcgtgg tgcgctgtat atgttcccga aaatcgacgc
    19921 caaacgcttt aacattcacg acgatcagaa aatggtgctg gatttcctgt tgcaggaaaa
    19981 agttctgttg gtgcaaggga cggcattcaa ctggccgtgg ccggatcact tccgcattgt
    20041 cacgctaccg cgtgtcgatg atatcgagct gtctttgagc aagttcgcgc gtttcctttc
    20101 tggttatcat cagctgtaat cttaatttca ctgccggaga ttgcatccgg cagcgttatc
    20161 ccgccacaat gacctgatga tgtcatcata cgtaaggtca ctatgaaaca gagccatttt
    20221 tttgcccatc tctcccgcct gaaactcatt aaccgctggc cgctaatgcg caacgtgctg
    20281 acggaaaatg tgtccgaaca cagtttgcag gtagcgatgg tcgcccatgc gctggcagct
    20341 atcaaaaatc gaaaatttgg cggtaatgtc aacgccgaac gtatcgcttt actggcgatg
    20401 taccacgatg cctcagaagt gctcaccggc gatctcccta ctccggtgaa atacttcaat
    20461 tcgcaaatcg ctcaggagta caaggctatt gaaaaaatcg ctcagcaaaa actggtcgat
    20521 atggttccgg aagagctgcg ggatatcttt gcgccgttaa ttgacgagca tgcatatagc
    20581 gatgaagaaa aatcgctggt gaaacaggca gatgcactgt gtgcatatct gaaatgtctg
    20641 gaagaactcg cggccggaaa taatgaattc ttgctggcaa aaacgcgact ggaagcgacg
    20701 cttgaagcgc gtcgcagcca ggagatggac tacttcatgg aagtatttgt tcccagcttc
    20761 catctttcgc tcgatgagat tagccaggat tcaccgctgt aagcagccgg agtctgcgtc
    20821 gcatcaggca ataagcgccg gatgcgacat caggctcttg tcaaaacgga aacagcatcg
    20881 ggatcatcac cacacaaacc gccatcacga taatggtgaa cggtaccccc aacttcacaa
    20941 agtcactgaa gctgtaattt cccggaccta aaaccagtgt gttaacaggt gaagaaaccg
    21001 gtgtcataaa ggcggcggat gctgccatcg ccacgaccat cgcgaatgga taaggcgaca
    21061 cccccatcgt tttggcagca gccagcgcaa tcggagccat caacaccgcc gtcgcggtat
    21121 tagagataaa tagcccaata accgccgaca agacaaacaa acaccccagc atcatatgtg
    21181 gcccgtaacc accgccaatg tccatcagcc ctttcaccgc cagcgcgaca cctcccgttt
    21241 tctgtaatgc cacagcaaac ggcatcatcc caacgatcaa aataatgctc ggccagtgaa
    21301 tggatttata ggcgctttca gcatctatac agcggaattt ccccatcagc aggcaggcga
    21361 tgatagcggc aacaggatta ggaatttcat ctgtcagcat taacgccacc attagcacca
    21421 gacagaaaat ggcatggggt gcctggctgt gcgcgggcga tgcttcactc acctcttccg
    21481 gtaagttcag cgctacgaag tcgcggccct gtttggccag cataccgatc agtttccagt
    21541 tacccacaac caggatgata tcgcccagca gcagaggctc atccgccagc gaaccttcca
    21601 gcgccacgcc attgcgcttt agccccacca cattcagtcc gtagcgggta cgaaaaccaa
    21661 tttcgcgcac cgatttacca atcagttctg actcaggaat taatgaaatc tctgccatgc
    21721 ccacatcaag ggcctggtca gaaaaatact cgccgcgcag taccatcggc tccagcaatt
    21781 gctcactaca aaattgccgg agatcgacat cagccgcaga catatcaata agcaaaacgt
    21841 cacgcgcgcg aaattcagaa accccattaa cgttcacgat aacgcgacga aaacgccgcc
    21901 agcgttcaac accgatgacg ttagcgccat aacgctcacg taatttgaga tcatccagcc
    21961 gttgaccaat cattggcgat ccggggcgaa tagccagacg tcgcgcacgc ccggtcagtc
    22021 gatattcacg gataagatcg cgaaaggttc gacgcgtcca gccttcgcgc tgcggggtct
    22081 gggtatcccc tttcagcatg aaacgcatca ctaacatata caaaataccc agcaccagca
    22141 cgaccaggcc aacaggtgtt acgctaaaga aactgaagcc gtgatagcct tcacgcagca
    22201 attcactgtt gactaccagg ttcggcggcg tcgccaccag cgtcatcatg ccgctaatca
    22261 gcccggcaaa acttaacggc atcatcagac gtgacggcga agtttgcata cgcatggcaa
    22321 cgcttaacac cacgggaata aagatagcga caacgccggt tgaactcata aacgcgccaa
    22381 gccccgcgac ggtcagcatc aacaaaacca acattttgat ttcactgttg cccgccactt
    22441 tgaccagcca tgttcccatt acggtggcaa caccggtacg gaccaaacca tcgccaataa
    22501 taaacaaggc ggcaatcagg acaacgttag gatcagaaaa gccggaaaat acttcgggga
    22561 ccgtcagcgt tccgcttaat gcaaacgcga caataacaaa caaagcgacc gcatccatac
    22621 gcactctgcc cgtcgcaaac aagacgatgg caacggccag taatgaaaga acccaaatca
    22681 attcaccgtt cacaacttat ccttgttaat tgagggggat gacttgattc tgccataaaa
    22741 aagccccgac gagacggggc taaatcatga tcaggtgttt cactgaataa taacatcgcc
    22801 atttagctgt ttggtcacag taatttgctc cagactgtgg aggaccaaat cgacctcatt
    22861 caggcgcggg gtatctgccg gagcgttaac cgcaatgaca tggcaacccg ccgccaggcc
    22921 agaaagcacg ccagcgggag catcttccgc caccacacac tcctgcggcg caagccccag
    22981 cagctgcgca cctaacagat acgcatcagg ttctggtttt ccgcgcttca ctcgctcagc
    23041 ggttacaaac acctttggtg cgggaagccc agctatttta tggcgcgctc gcgctaccgg
    23101 catggagcca gaagtcacaa tggcccacgg aatacctgct ttattcaaat gattgagtaa
    23161 ggcgattgcc cccggaagcg cggtaatacc ttcggtttcc gtggcctcga tgtgctccag
    23221 acacgtaaac tcggcggcaa tatcagcctc ggatttgccc gccataaaat ggcgcaaaga
    23281 ggtgatcgcc tgtttaccgt gaatgaaagc cagcacctct tccggcgcta acccatgacg
    23341 tctggcccag ttgctccacg cccgttctac cgcaggcagg gaatccacca gcgttccatc
    23401 aagatcaaac agaaaacctt tgcaccgcac gcgggcctcc tcaggcgttg ataatttgat
    23461 taatttcgtt ggcgctcaaa tggtactgac gcaggcaggc atgccacaca ttaagcatgc
    23521 gctgatattt ttcccacatt ggcgtctggg cgttaaaacc atgagtccca gcgtcaaaat
    23581 gggtatagcg cccttccaca ttaaccataa agcggacata accgaggtaa cgtgcttcag
    23641 tggcggcgtc aaagccgagg aaggtgacac gacgttcatc gatggattgc tggtcctgca
    23701 aattagacca ggaaacatgc aacgcatgat acatctccat aatgtcgatg atagtgcggc
    23761 aggtttcttc tttcagctcg ccaaactcgc gatccaattc acgcatctgt aatccgtaac
    23821 cacgctcaat aattgtttgc aggcgacggt aacgttcagc atttgccgga tcgagcatag
    23881 tcatcatctt gtactgatta gacaaaataa gacgttgcgc gttggtcatt tccattgttg
    23941 actcctgtat cactctacta cggtgaaaaa aaagaaggct gagtatgcct tcttttatat
    24001 gcgtaatcag gggtcaatta caaatcatca aggaaagttt tatccagttg tttgaaggcg
    24061 cgcttaagcg tgtcagctaa tgcctggtaa tcaggcttgc cttcaacggg tgccaacacc
    24121 tgtccagact cctgcaattt accgcgaact tcataaaacc agttgaggat agcagggggt
    24181 aatggcgtta cagaacgctt gcccagccac cataatccct gcatgggtaa acttaaggcg
    24241 aacagcgcag tggcaactgc cggcccaagc tgaccgccca gggcaatctg ccagcagaga
    24301 gtaaatacgg cgatcggcgg cataaaacgg atcgcataac gcgtcatctt gataacgcga
    24361 ttttcgacaa agaccggggc aaggcgtttt tccagcggcc acgtctttga gtaatgctgt
    24421 ccccggcgaa acaagctaaa aaaattaaca gaacgattat ccggcgttga catgcttcac
    24481 ctcaacttca catataaaga ttcaaaaatt tgtgcaaatt cacaactcag cgggacaaca
    24541 ttcaaaacat tttgtcttcc atacccacta tcaggtatcc tttagcagcc tgaaggccta
    24601 agtagtacat attcattgag tcgtcaaatt catatacatt atgccattgg ctgaaaatta
    24661 cgcaaaatgg catagactca agatatttct tccatcatgc aaaaaaaatt tgcagtgcat
    24721 gatgttaatc ataaatgtcg gtgtcatcat gcgctacgct ctatggctcc ctgacgtttt
    24781 tttagccacg tatcaattat aggtacttcc atgtcgagta agttagtact ggttctgaac
    24841 tgcggtagtt cttcactgaa atttgccatc atcgatgcag taaatggtga agagtacctt
    24901 tctggtttag ccgaatgttt ccacctgccc gaagcacgta tcaaatggaa aatggacggc
    24961 aataaacagg aagcggcttt aggtgcaggc gccgctcaca gcgaagcgct caactttatc
    25021 gttaatacta ttctggcaca aaaaccagaa ctgtctgcgc agctgactgc tatcggtcac
    25081 cgtatcgtac acggcggcga aaagtatacc agctccgtag tgatcgatga gtctgttatt
    25141 cagggtatca aagatgcagc ttcttttgca ccgctacaca acccggctca cctgatcggt
    25201 atcgaagaag ctctgaaatc tttcccacag ctgaaagaca aaaacgttgc tgtatttgac
    25261 accgcgttcc accagactat gccggaagag tcttacctct acgccctgcc ttacaacctg
    25321 tacaaagagc acggcatccg tcgttacggc gcgcacggca ccagccactt ctatgtaacc
    25381 caggaagcgg caaaaatgct gaacaaaccg gtagaagaac tgaacatcat cacctgccac
    25441 ctgggcaacg gtggttccgt ttctgctatc cgcaacggta aatgcgttga cacctctatg
    25501 ggcctgaccc cgctggaagg tctggtcatg ggtacccgtt ctggtgatat cgatccggcg
    25561 atcatcttcc acctgcacga caccctgggc atgagcgttg acgcaatcaa caaactgctg
    25621 accaaagagt ctggcctgct gggtctgacc gaagtgacca gcgactgccg ctatgttgaa
    25681 gacaactacg cgacgaaaga agacgcgaag cgcgcaatgg acgtttactg ccaccgcctg
    25741 gcgaaataca tcggtgccta cactgcgctg atggatggtc gtctggacgc tgttgtattc
    25801 actggtggta tcggtgaaaa tgccgcgatg gttcgtgaac tgtctctggg caaactgggc
    25861 gtgctgggct ttgaagttga tcatgaacgc aacctggctg cacgtttcgg caaatctggt
    25921 ttcatcaaca aagaaggtac ccgtcctgcg gtggttatcc caaccaacga agaactggtt
    25981 atcgcgcaag acgcgagccg cctgactgcc tgatttcaca ccgccagctc agctggcggt
    26041 gctgttttgt aacccgccaa atcggcggta acgaaagagg ataaaccgtg tcccgtatta
    26101 ttatgctgat ccctaccgga accagcgtcg gtctgaccag cgtcagcctt ggcgtgatcc
    26161 gtgcaatgga acgcaaaggc gttcgtctga gcgttttcaa acctatcgct cagccgcgta
    26221 ccggtggcga tgcgcccgat cagactacga ctatcgtgcg tgcgaactct tccaccacga
    26281 cggccgctga accgctgaaa atgagctacg ttgaaggtct gctttccagc aatcagaaag
    26341 atgtgctgat ggaagagatc gtcgcaaact accacgctaa caccaaagac gctgaagtcg
    26401 ttctggttga aggtctggtc ccgacacgta agcaccagtt tgcccagtct ctgaactacg
    26461 aaatcgctaa aacgctgaat gcggaaatcg tcttcgttat gtctcagggc actgacaccc
    26521 cggaacagct gaaagagcgt atcgaactga cccgcaacag cttcggcggt gccaaaaaca
    26581 ccaacatcac cggcgttatc gttaacaaac tgaacgcacc ggttgatgaa cagggtcgta
    26641 ctcgcccgga tctgtccgag attttcgacg actcttccaa agctaaagta aacaatgttg
    26701 atccggcgaa gctgcaagaa tccagcccgc tgccggttct cggcgctgtg ccgtggagct
    26761 ttgacctgat cgcgactcgt gcgatcgata tggctcgcca cctgaatgcg accatcatca
    26821 acgaaggcga catcaatact cgccgcgtta aatccgtcac tttctgcgca cgcagcattc
    26881 cgcacatgct ggagcacttc cgtgccggtt ctctgctggt gacttccgca gaccgtcctg
    26941 acgtgctggt cgccgcttgc ctggcagcca tgaacggcgt agaaatcggt gccctgctgc
    27001 tgactggcgg ctacgaaatg gacgcgcgca tttctaaact gtgcgaacgt gctttcgcta
    27061 ccggcctgcc ggtatttatg gtgaacacca acacctggca gacctctctg agcctgcaga
    27121 gcttcaacca ggaagttccg gttgacgatc acgaacgtat cgagaaagtt caggaatacg
    27181 ttgctaacta catcaacgct gactggatcg aatctctgac tgccacttct gagcgcagcc
    27241 gtcgtctgtc tccgcctgcg ttccgttatc agctgactga acttgcgcgc aaagcgggca
    27301 aacgtatcgt actgccggaa ggtgacgaac cgcgtaccgt taaagcagcc gctatctgtg
    27361 ctgaacgtgg tatcgcaact tgcgtactgc tgggtaatcc ggcagagatc aaccgtgttg
    27421 cagcgtctca gggtgtagaa ctgggtgcag ggattgaaat cgttgatcca gaagtggttc
    27481 gcgaaagcta tgttggtcgt ctggtcgaac tgcgtaagaa caaaggcatg accgaaaccg
    27541 ttgcccgcga acagctggaa gacaacgtgg tgctcggtac gctgatgctg gaacaggatg
    27601 aagttgatgg tctggtttcc ggtgctgttc acactaccgc aaacaccatc cgtccgccgc
    27661 tgcagctgat caaaactgca ccgggcagct ccctggtatc ttccgtgttc ttcatgctgc
    27721 tgccggaaca ggtttacgtt tacggtgact gtgcgatcaa cccggatccg accgctgaac
    27781 agctggcaga aatcgcgatt cagtccgctg attccgctgc ggccttcggt atcgaaccgc
    27841 gcgttgctat gctctcctac tccaccggta cttctggtgc aggtagcgac gtagaaaagg
    27901 ttcgcgaagc aactcgtctg gcgcaggaaa aacgtcctga cctgatgatc gacggtccgc
    27961 tgcagtacga cgctgcggta atggctgacg ttgcgaaatc caaagcgccg aactctccgg
    28021 ttgcaggtcg cgctaccgtg tttatcttcc cggatctgaa caccggtaac accacctaca
    28081 aagcggtaca gcgttctgcc gacctgatct ccatcgggcc gatgctgcag ggtatgcgca
    28141 agccggttaa cgacctgtcc cgtggcgcac tggttgacga tatcgtctac accatcgcgc
    28201 tgactgcgat tcagtctgca cagcagcagt aatctcgtca tcatccgcag ctttgcgctg
    28261 cggatatctg aaccggaaat aatcactatt tccggttttt tattctctta atctgcatta
    28321 atcctttctg attatcttgc ttaactgcgc tgcatcaatg aattgcgcca tttcactttg
    28381 catacttacc actttgtttt gtgcaaggga atatttgcgc tatgtccgca atcactgaat
    28441 ccaaaccaac aagaagatgg gcaatgcccg atacgttggt gattatcttt tttgttgcta
    28501 ttttaaccag ccttgccacc tgggtagttc cggtggggat gtttgacagt caggaagtgc
    28561 agtatcaggt tgatggtcaa acaaaaacac gcaaagtcgt agatccacac tcatttcgca
    28621 ttctgactaa cgaagcaggc gaacctgagt atcaccgcgt acagctgttc acgacgggcg
    28681 atgaacgccc gggcctgatg aacttcccgt ttgaaggatt aacctcagga tcgaaatacg
    28741 ggacagctgt tggcatcatc atgtttatgc tggtgattgg cggcgcgttt ggcattgtga
    28801 tgcgtacagg aaccattgat aacggtatcc tggcgcttat tcgccatacc cgcgggaatg
    28861 aaattctctt tattcctgcg ctgtttattc tgttttcact tggcggcgcg gtatttggta
    28921 tgggagaaga ggccgtcgcc tttgccatta tcatcgcacc gctaatggtc cggctgggct
    28981 atgacagtat taccaccgtc ctggtgacct atattgccac gcaaatcggt tttgccagtt
    29041 cgtggatgaa cccgttttgt gtggtcgttg ctcaggggat tgccggcgtt ccggtgcttt
    29101 ctggctccgg gttgcgcatc gtggtgtggg ttatcgccac tatgattggc ctgatcttta
    29161 ccatggtgta cgcctcacga gtgaaaaaga atcctcttct gtcacgcgtg catgagtccg
    29221 accgcttctt tcgtgaaaag caggcggatg ttgaacaacg tccgtttacc tttggtgact
    29281 ggctggtatt gattgtcctg accgccgtaa tggtctgggt gatttggggc gtgatcgtta
    29341 atgcctggtt tattccagaa attgccagcc agttcttcac catgggtctg gtgattggca
    29401 tcatcggcgt tgtttcccgc cttaacggca tgacggttaa taccatggct tcatccttta
    29461 ccgaaggggc gcgaatgatg atcgcccctg ccctgctggt gggtttcgcc aaagggattt
    29521 tgctgctggt cggtaatggt gaagcgggtg atgccagcgt gttaaatacc atcctcaaca
    29581 gcattgccaa tgccattagc ggtctggaca acgcggtcgc ggcctggttt atgttgctct
    29641 tccaggcggt atttaatttc ttcgtgacgt ccggttctgg tcaggcggcg ttaaccatgc
    29701 cgttactggc accgcttggc gatctggtcg gtgttaaccg tcaggttacc gtgctggcat
    29761 tccagtttgg tgatggcttc agtcacatca tttacccaac ctcagcttcg ttaatggcaa
    29821 ctctcggtgt gtgcagggtg gacttccgta actggctgaa ggtgggcgcg accctacttg
    29881 gactgctgtt tattatgtcc agcgtcgtag tgatcggcgc tcagttgatg ggctaccact
    29941 aaaaatgtta agagccgcaa ttgcggctct tttttcattc tgccgtttca gtctctacgg
    30001 cttcattttt ggcattgcgc ttcatccaca acgccagcgc tttcagcgag tctggagtga
    30061 actcatcgca gcgtgcggtg atttcttccg gcgtcagcca gcaaacttca ctgacttcat
    30121 cttcctgtag ggcgaaggga ccgtgagaga cgcagctgaa caacgcgccc cagacacggc
    30181 aatttttatc ttcgaaatag aactgcccgt gctcggcaaa ggggacaccg gcaatgccca
    30241 actcttcttc cgcttcgcga cgcgcggatt ccagcagttg ctcatcggcc tgaaccacac
    30301 cgcctgcggt cgcatctaac atgccgggta aaaagtcttt tgtctcggta cgacgctgga
    30361 ccagaatttt gcccatgcca tcatgcacga cgatgtaagt tgcacgatga cgcagacact
    30421 gtgcccgcat ttgttcccgg ctggcttgtg caatgacttc gttctcttca ttgacaatat
    30481 ccacccattc agtacttgcc aaacgacgct gttccattat caggaaacct tctttttctg
    30541 gcgctcttac ggcgcatttt gagttgtggg taaattacgg attaatcgcg acctgcgcaa
    30601 tgatactttg atcattgagt gcgattacac taagtacgtc attatccagc atgccataac
    30661 tcgccggatt accgcctttc ggaatactca ccgagccggg gttgaagtgg aaaatctccc
    30721 cccgctgttc tgccactggc agatgggtat gaccgtacac cagcacatcg ttctggttta
    30781 aagcaggtag attttccggg ccaaaaagat gaccatgcgt caaaaacaga cgttgttttt
    30841 ccagtaatac ctgttgccac ggcgcggtta tcgggaaatg cagcagcatt tgatccactt
    30901 cgctgtcgca gttgccgcgc acagcgataa ccttatgtgc cacttcatta agccgttcag
    30961 cgactttggc tggcgcgtaa ccctccggta aagcattacg cgggccgtga ttcaacacgt
    31021 cgccaaggat caccagccac tgggcaccgc tttgggcaaa caactccaga acacgttccg
    31081 tcgccggtaa cgacccatga atgtccgatg caaacatcag tttcatcact cactcctcgt
    31141 cgaaaaaacg taagctatga tactggattc tgtcgttgct atcagccaga ttgcttcgcc
    31201 gaaagtgcaa taaaacgctg gacagacgct cgctgccact ggaatgtcgc ataatccacc
    31261 aggcgttctg gcacctcatc gccatgtagt accaggcggt taatcattag cgccagatca
    31321 gtatcagcaa tgcaccattc accaaataaa ttcggctgac ccagtgctaa caaatgttct
    31381 gccatcgcga cactggcttt tccctcggcc gttagtggcg cttttttcgc ccccgcaaag
    31441 acaacatccg tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag ccaggcctga
    31501 atctgtcgtg cacgcgcacg attttctaaa tcaagcggat aaatacgttc ccaggtcggt
    31561 ggcgcaaatc gatcttccag atactccgca atggcagaag attcactcaa ctcaaaatca
    31621 tcgatttgta ataacggcac acggcgtgtc tgaccgtaac cttgccacgt cggctgcaaa
    31681 tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg acaggccttt ttcctgcaac
    31741 gccacccaag cggataacac ataaggggag aaaaagtggg catctgacca aagcgtgata
    31801 gcgggtttac tcattatgtc ctcggcaata aaggaggctg gctatcaaac tatagagcct
    31861 ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta tacttgtggt
    31921 gttcagatga ttaaacaacc ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca
    31981 atggtcacaa aattacgctg tttctcgaag aagcagagct tgattatcgc ttgattaagg
    32041 tagacctggg gaaaggcggt cagtttcgtc cggaattttt gcgcatttcg cccaacaaca
    32101 aaattccggc aattgttgat cattctcctg ccgatggcgg cgaaccgcta agcctctttg
    32161 aatctggtgc cattttgttg tatctggctg agaaaacggg gctctttttg agtcatgaaa
    32221 cgcgtgaacg cgccgccaca ttacagtggt tattctggca ggtaggcgaa ctggggccga
    32281 tgcttgggca aaatcatcat tttaatcacg cagcccccca aaccattcct tacgctattg
    32341 aacgttatca ggttgaaact cagcgtcttt accatgtact aaacaagcgg ctggaaaact
    32401 cgccctggct gggaggcgag aactacagca ttgcggatat tgcctgctgg ccgtgggtta
    32461 atgcctggac tcgccagcga attgacctcg caatgtatcc ggcagtcaag aactggcatg
    32521 agcggatccg ttcgcgccct gccaccgggc aggcactgct aaaagcacaa ctcggtgatg
    32581 agcgttcgga tagttaacag aaacaggttc tcgtgtatta tttcatccta agtaaaacaa
    32641 cggagaacct gcaatggcac aacctgccgc tattattcgt ataaagaacc ttcgtttacg
    32701 tacgtttatc ggaattaagg aagaagaaat taacaaccgt caggatattg ttatcaatgt
    32761 gacgatccac taccccgccg ataaagcgcg cactagcgaa gatatcaacg atgcgctgaa
    32821 ttatcgcacc gtaacgaaaa acattattca gcatgtagag aataaccgtt tctctttgtt
    32881 ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa catcactggg tgacgtatgc
    32941 tgaagtggag atcgataaac tgcacgcgct gcgctacgcc gattcggtat ccatgacctt
    33001 aagctggcag cgttaaccgc catatcggga ggctgcatga acatagtgat caccggaggg
    33061 acgggattaa ttggtcgcca tttgattccg cgtttgctgg agctgggcca tcaaatcacg
    33121 gtagtgacgc gtaacccaca gaaagccagt tccgttctcg gccctcgggt gacgctatgg
    33181 caagggctag ccgatcaaag caacctcaac ggcgttgatg cggtaatcaa cctggccgga
    33241 gaaccgattg ctgataaacg ctggactcac gagcaaaaag agcgtctctg ccaaagccgc
    33301 tggaatatca cgcaaaaact ggtcgatttg attaatgcca gcgacacgcc gccgtcggta
    33361 ctcatttccg gctcggcaac gggctattat ggcgacttag gtgaagtggt ggttaccgaa
    33421 gaggaaccgc cgcataacga atttactcat aaactctgcg cccgctggga agagattgcc
    33481 tgccgggcgc aaagtgacaa aacgcgagtg tgcctgctgc gaaccggcgt ggtactggca
    33541 ccggacggcg gtattctcgg taaaatgctg ccaccgtttc gtcttggcct gggcgggccg
    33601 attgactctg gtcggcagta tctggcctgg attcatattg atgatatggt caacggtatt
    33661 ctctggctgc tggataacga gctgcgcggg ccatttaata tggtttcgcc ctacccggta
    33721 cgcaatgaac aatttgccca tgcgctcggt cacgcgctgc atcgcccggc cattttgcgc
    33781 gtccccgcga ccgccattcg actgttaatg ggcgaatctt cagtactggt attaggtgga
    33841 caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg cgtttcgctg gtacgattta
    33901 gaagaggcgc tggcggatgt cgttcgctga tgtggtttac agcaaacatc cgccagttaa
    33961 ctcccggtgt tacaggatta gtggctttgc gcgataagat cgtctggtga aagtcgggtc
    34021 accatcataa ctaactctct gtctaaacct ctatccagca tctcctgagc aatacgcagg
    34081 gcttcttcgt gtttgccctg cattgcgcct tcttcacgta atctgtcagc aatggtcatc
    34141 agtttctcct tttcttgtgg tgcgcgttcc gctatctcac caataaatgc acgaaaacgc
    34201 tgggcatccc ctgtttgtaa tacgtaatta aacagggctt ttagctgtct gtcattagtg
    34261 ttccctgtaa ctagcagcga aacaatttgg tcgactaatc ccaacagatc gcgctgacga
    34321 atatgtttct gaattaactc caacagcgcc attttgcggt gttgcataat ctcgtcatcc
    34381 ggcaccacgg taatatccac caacggaaac gctgatgaat atattttgcg ggctatagca
    34441 ggctcggcaa attcatcaag cca
//
LOCUS       DAJTSL010000028        34161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTSL010000028 DAJTSL010000000
VERSION     DAJTSL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.028"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..152)
                     /locus_tag="OO433_001178"
     CDS             complement(<1..152)
                     /locus_tag="OO433_001178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049724.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNP"
     gene            complement(312..524)
                     /gene="rpmE"
                     /locus_tag="OO433_001179"
     CDS             complement(312..524)
                     /gene="rpmE"
                     /locus_tag="OO433_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6049725.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            727..2925
                     /gene="priA"
                     /locus_tag="OO433_001180"
     CDS             727..2925
                     /gene="priA"
                     /locus_tag="OO433_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6049726.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWILGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3081..4106
                     /gene="cytR"
                     /locus_tag="OO433_001181"
     CDS             3081..4106
                     /gene="cytR"
                     /locus_tag="OO433_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6049727.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4198..5157
                     /gene="ftsN"
                     /locus_tag="OO433_001182"
     CDS             4198..5157
                     /gene="ftsN"
                     /locus_tag="OO433_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6049728.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHNKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5250..5780
                     /gene="hslV"
                     /locus_tag="OO433_001183"
     CDS             5250..5780
                     /gene="hslV"
                     /locus_tag="OO433_001183"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6049729.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5790..7121
                     /gene="hslU"
                     /locus_tag="OO433_001184"
     CDS             5790..7121
                     /gene="hslU"
                     /locus_tag="OO433_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6049730.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7188..8114
                     /gene="menA"
                     /locus_tag="OO433_001185"
     CDS             7188..8114
                     /gene="menA"
                     /locus_tag="OO433_001185"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6049731.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDRPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8207..8692
                     /gene="rraA"
                     /locus_tag="OO433_001186"
     CDS             8207..8692
                     /gene="rraA"
                     /locus_tag="OO433_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6049732.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="OO433_001187"
     CDS             complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="OO433_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6049733.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9448..10293
                     /gene="glpF"
                     /locus_tag="OO433_001188"
     CDS             9448..10293
                     /gene="glpF"
                     /locus_tag="OO433_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR6049734.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVKEKETTTPSE
                     QKASL"
     gene            10316..11824
                     /gene="glpK"
                     /locus_tag="OO433_001189"
     CDS             10316..11824
                     /gene="glpK"
                     /locus_tag="OO433_001189"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6049735.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRSE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11959..12969
                     /gene="glpX"
                     /locus_tag="OO433_001190"
     CDS             11959..12969
                     /gene="glpX"
                     /locus_tag="OO433_001190"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6049736.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13066..13812
                     /gene="fpr"
                     /locus_tag="OO433_001191"
     CDS             13066..13812
                     /gene="fpr"
                     /locus_tag="OO433_001191"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6049737.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13817..14245)
                     /gene="uspD"
                     /locus_tag="OO433_001192"
     CDS             complement(13817..14245)
                     /gene="uspD"
                     /locus_tag="OO433_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6049738.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14272..14571)
                     /locus_tag="OO433_001193"
     CDS             complement(14272..14571)
                     /locus_tag="OO433_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6049739.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSVDEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14783..15223)
                     /locus_tag="OO433_001194"
     CDS             complement(14783..15223)
                     /locus_tag="OO433_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6049740.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     CFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15324..15923
                     /locus_tag="OO433_001195"
     CDS             15324..15923
                     /locus_tag="OO433_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6049741.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16031..16798
                     /gene="tpiA"
                     /locus_tag="OO433_001196"
     CDS             16031..16798
                     /gene="tpiA"
                     /locus_tag="OO433_001196"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6049742.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16853..17608)
                     /gene="cdh"
                     /locus_tag="OO433_001197"
     CDS             complement(16853..17608)
                     /gene="cdh"
                     /locus_tag="OO433_001197"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6049743.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17715..18704)
                     /gene="sbp"
                     /locus_tag="OO433_001198"
     CDS             complement(17715..18704)
                     /gene="sbp"
                     /locus_tag="OO433_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6049744.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVMRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19024..19986)
                     /gene="pfkA"
                     /locus_tag="OO433_001199"
     CDS             complement(19024..19986)
                     /gene="pfkA"
                     /locus_tag="OO433_001199"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6049745.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20167..21069)
                     /gene="fieF"
                     /locus_tag="OO433_001200"
     CDS             complement(20167..21069)
                     /gene="fieF"
                     /locus_tag="OO433_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6049746.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMIDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21217..21729)
                     /gene="cpxP"
                     /locus_tag="OO433_001201"
     CDS             complement(21217..21729)
                     /gene="cpxP"
                     /locus_tag="OO433_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6049747.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQRS
                     TQSHMFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQ
                     AEKMANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLK
                     LLSSSNSRSQ"
     gene            21879..22577
                     /gene="cpxR"
                     /locus_tag="OO433_001202"
     CDS             21879..22577
                     /gene="cpxR"
                     /locus_tag="OO433_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6049748.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22574..23947
                     /gene="cpxA"
                     /locus_tag="OO433_001203"
     CDS             22574..23947
                     /gene="cpxA"
                     /locus_tag="OO433_001203"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6049749.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24053..24727)
                     /gene="yiiM"
                     /locus_tag="OO433_001204"
     CDS             complement(24053..24727)
                     /gene="yiiM"
                     /locus_tag="OO433_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6049750.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24876..25841)
                     /gene="kdgT"
                     /locus_tag="OO433_001205"
     CDS             complement(24876..25841)
                     /gene="kdgT"
                     /locus_tag="OO433_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6049751.1"
                     /translation="MRKSRGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMITGTVSI
                     LAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVEVGFFAG
                     LSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGTAGIASF
                     EPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTVIAQTGL
                     LGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPMLIAEMVPAFKP
                     MAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26120..26740)
                     /gene="sodA"
                     /locus_tag="OO433_001206"
     CDS             complement(26120..26740)
                     /gene="sodA"
                     /locus_tag="OO433_001206"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6049752.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27024..28058
                     /gene="rhaT"
                     /locus_tag="OO433_001207"
     CDS             27024..28058
                     /gene="rhaT"
                     /locus_tag="OO433_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR6049753.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLLNTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28055..28921)
                     /gene="rhaR"
                     /locus_tag="OO433_001208"
     CDS             complement(28055..28921)
                     /gene="rhaR"
                     /locus_tag="OO433_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6049754.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(28977..29813)
                     /gene="rhaS"
                     /locus_tag="OO433_001209"
     CDS             complement(28977..29813)
                     /gene="rhaS"
                     /locus_tag="OO433_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6049755.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30101..31513
                     /gene="rhaB"
                     /locus_tag="OO433_001210"
     CDS             30101..31513
                     /gene="rhaB"
                     /locus_tag="OO433_001210"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6049756.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGLDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELAQLRGEDFSQLHIVGGGCQNTLLNQ
                     LCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDS
                     EIAHYVAQLHSTRQTKELCA"
     gene            31510..32769
                     /gene="rhaA"
                     /locus_tag="OO433_001211"
     CDS             31510..32769
                     /gene="rhaA"
                     /locus_tag="OO433_001211"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6049757.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWGEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISKKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDCDHVVLLDDETQAIASEIVRHDLFDQVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            33036..33860
                     /gene="rhaD"
                     /locus_tag="OO433_001212"
     CDS             33036..33860
                     /gene="rhaD"
                     /locus_tag="OO433_001212"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR6049758.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNVHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33870..>34161
                     /gene="rhaM"
                     /locus_tag="OO433_001213"
     CDS             33870..>34161
                     /gene="rhaM"
                     /locus_tag="OO433_001213"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6049759.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASADVCQRWWKYMTDVMPANPDNSPVSSEL"
BASE COUNT         8370 a         8915 c         8867 g         8009 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcaccccg ccggggcaca ctgaacaggc cacgccggtg
       61 ggagcaccaa tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgccgccgg ttttccgctc atcgtaaaat ccttttgaaa taaataagaa
      181 ccagaagcct agcgccagtc aacaacagga aatgataatg agttaacacc ccggaatttt
      241 tcagaattta taaaaggcaa aattcaggca caaaaaaagc gccgtgcggc gcttttttcg
      301 gaaatccggt cttatttgct gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg
      361 ccaccggtag caacatcacg ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg
      421 tcgaggttca ggtcatgacc aacagtggag cggattttca ttacgttacc gcaagagcag
      481 ctagcagtaa tttcttcgta tttcgggtga atatcttttt tcatgggaaa acctcggttt
      541 aaggccgcgt cgctcttcca gccctaacgc cagacaccac gcgatgttaa aagtatagct
      601 tcaatacgat catttcgtac gaaggcgcga aatcatacag aaattaacca acgtatgcaa
      661 actgatccgc actcttctac ggcaatgtgt atactaaccc accgaatttc aagtcaggat
      721 gatgctatgc ccgttgccca cgttgccttg cccgttccgc ttcctcgtac ctttgactat
      781 ctgctgccag aaggcatgac ggttaaagcc gggtgtcgcg tgcgcgtgcc gtttggcaaa
      841 cagcaggagc gcatcggggt tgtggtgtca gttagcgatg tcagtgagct gccgcttaac
      901 gagctgaaag cggtagtgga agtgctggac gttgagccgg tatttactca ctccgtctgg
      961 cggttactgc tctgggcggc agattactat catcatccga ttggcgatgt gctgtttcat
     1021 gccttgccga ttttactgcg ccaggggcgg cctgcggcga acgcgccgat gtggtactgg
     1081 tttgccactg aacaaggcca ggcggtggat ctgaacagcc tgaaacgctc ccccaagcaa
     1141 caacaggcgc tggcggcgtt acggcaaggc aaaatctggc gcgaccaggt cgccacgctc
     1201 gaatttaatg atgccgcgtt gcaggcgcta cgcaaaaaag gtctgtgtga tttagcaagt
     1261 gaaacaccag agtttagcga ctggcgaacg aactatgccg tttctggtga gcggttgcga
     1321 ttgaataccg aacaggccac cgccgttggc gcaattcata gcgcggcaga tactttttct
     1381 gcctggctgc tggcgggcgt taccggttcc ggtaaaacgg aggtttatct cagtgtactg
     1441 gaaaacgtgc tcgctcaggg caaacaggcg ctggtgatgg tgccggaaat cggcctgaca
     1501 ccgcaaacta tcgcccgttt tcgtgaacgt tttaatgccc ccgtggaagt tctgcattcc
     1561 ggcctgaacg acagcgagcg gctttctgcg tggttgaaag cgaaaaatgg cgaggcggcg
     1621 attgtgatcg gcacccgctc cgcgctgttt acgccgttta aaaatctcgg cgtgattgtc
     1681 attgatgaag agcacgacag ctcctacaag cagcaggaag gctggcgcta tcatgcccgc
     1741 gacctggcgg tgtatcgtgc gcacagcgag caaatcccga ttattcttgg ctccgcaacg
     1801 cccgcgctgg aaacgttatg caacgtccag cagaaaaaat accgcctgct gcgcctgacc
     1861 cgtcgggcag ggaatgctcg tccggcaatt caacatgtgc tggatttaaa aggtcagaag
     1921 gtgcaggcag gtctggctcc ggcgttaatc acccgtatgc gccagcattt acaggccaac
     1981 aatcaggtta tcctctttct taatcgtcgg ggttttgcgc ctgcgctgct gtgccacgac
     2041 tgcggctgga tagccgaatg cccacgttgc gatcactact acacgctgca tcaggcgcaa
     2101 cagcatttgc gctgtcacca ctgtgacagt cagcgtccgg tgccgcgcca gtgcccttcc
     2161 tgcggttcca cgcacctggt ccccgtgggg ctgggcaccg aacagcttga acagacgctt
     2221 gcgccgatgt tccccggcgt gcccatttca cgtatcgacc gcgataccac cagccgcaaa
     2281 ggggcactgg aacagcaact ggcggaagtg catcgcggcg gcgcgaggat tttgatcggc
     2341 acacaaatgc tggcgaaagg tcaccacttt ccggatgtga cgctggtggc gttactggat
     2401 gtggacggcg cgctgttttc tgccgatttt cgctcggcag aacgtttcgc ccagctttac
     2461 acccaggtcg ccggtcgtgc cgggcgtgcg ggtaaacagg gcgaagtggt gctgcaaacg
     2521 caccatccgg aacatcctct gttgcaaacg ttgctctata aaggctacga cgcctttgcc
     2581 gaacaggcgc tggctgagcg acgcatgatg cagctgccgc cgtggaccag ccatgtgatt
     2641 gtgcgtgcgg aagatcataa caatcagcac gcgccgctgt tcctgcaaca actgcgtaat
     2701 ctgatccttt ccagcccgct ggcagacgac aaactgtgga ttctcggccc ggttccggct
     2761 ctggcaccta agcgtggtgg ccgctggcgc tggcagatct tgttgcagca cccttcccgc
     2821 gtgcgcttgc aacacatcat tagtggtacg ctggcgctta ttaatacaat accggattcc
     2881 cgcaaggtga aatgggtgtt ggatgtcgat cccatcgaag gctaatgcgc aaacgttgcg
     2941 aggcggatcg aaaaattcaa tattcatcac acttttcatg aaaattctgt aaccgttttc
     3001 acgcgctatc tgctaaaaat gttgccgatg tgaagtaaac atggatgtag tacgcctgac
     3061 gtaccaggcg aggagtgagt gtgaaagcga agaagcagga aactgccgcg accatgaaag
     3121 acgttgccct caaggcaaaa gtctctacag cgactgtctc ccgagcatta atgaatcccg
     3181 ataaagtctc ccaggccacc cgtaatcggg ttgaaaaagc ggcccgggaa gtgggttatt
     3241 taccgcagcc tatggggcgc aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg
     3301 tcccggatat ctgcgatccc ttctttagcg aaattattcg cggtatcgaa gttacggcgg
     3361 caaatcacgg atatctggtg ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa
     3421 cctttatcga tttgatcatc accaagcaaa ttgatggcat gttgctgctg ggttcaaggc
     3481 tgccgtttga tgccagcatt gaggaacagc gtaatctgcc gccgatggtg atggcgaacg
     3541 aatttgcacc ggaactggag ctgcctaccg ttcatatcga caatctgacc gccgcatttg
     3601 atgcagtaaa ttatttatat gagcaagggc ataaacggat tggctgtata gccggtcccg
     3661 aagagatgcc actgtgtcac taccgcctgc aaggctatgt tcaggcgctg cgtcgctgcg
     3721 gaattatggt tgatccacaa tacatcgccc gtggcgactt caccttcgaa gccggaagca
     3781 aagcaatgca acagctgctt gatcttccac aaccgcctac tgcagtcttc tgccatagcg
     3841 atgtgatggc gctcggcgca ctttctcagg caaaacgcca ggggctgaaa gtcccggaag
     3901 acctttccat aatcggtttt gataacatcg acctgacgca attttgtgat ccgccgctga
     3961 caaccatcgc gcagccgcgt tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc
     4021 aaatgcaggg gcaacacgtt ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc
     4081 ggggatcaac acgcgcgtta ccttaaagta aacctttaag actgccgtga ctggtcaaag
     4141 gcccgccgct taagtaacat ggcgggctga cgaacgaata aatacagcga aacgatagtg
     4201 gcacaacgag attatgtacg ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc
     4261 tcacggaaaa agcaacgaaa tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc
     4321 gccgttcttg tgacctttat cggtggtctg tacttcatta cgcatcacaa taaagaagag
     4381 tccgagacgc tgcaaagcca gaaagtgacc ggaaacggac taccaccaaa accagaagaa
     4441 cgctggcgct acattaaaga gctggaaagt cgccagccgg gtgtgcgtgc gcccacagaa
     4501 ccttctgccg gtggtgaagt gaaaacgccg gagcaactga caccagaaca acgtcagctt
     4561 cttgaacaaa tgcaggctga tatgcgccag cagccaacgc agctggttga ggttccgtgg
     4621 aatgaacaga cgccggaaca gcgtcagcaa acgctacaac gccagcgtca ggcgcagcaa
     4681 ttagcggagc agcaacgcct ggctcagcag tcgcgtacga ctgaacaaag ctggcagcag
     4741 cagacgcgta cgtcgcaagc cgcgccggta caggcgcaac cgcgccagtc taaaccagct
     4801 tctactcagc agccgtacca ggatctgctg caaacccctg cgcacacgac cgcgcagtcg
     4861 aagccgcagc aagctgcacc ggttgctcgt gccgctgacg cgccaaaacc gacggcggag
     4921 aaaaaagacg aacgccgctg gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca
     4981 gagacggtac gtgctcagct ggcgttcgaa ggatttgact cgaaaatcac caccaacaat
     5041 ggctggaatc gtgtggtcat tggcccggtg aaaggcaaag agaacgcaga cagcaccctc
     5101 aatcggttga agatggcggg tcatacaaac tgcattcggc tcgccgccgg gggttgaaac
     5161 cctcaaaatc ccccccatct ataattgcat tatgcctcgt acttttgtac ggggtttgta
     5221 ctctgtattc gtaaccaagg ggtcagctcg tgacaactat agtaagcgta cgccgtaacg
     5281 gccatgtggt catcgctggt gatggtcagg ccacgttggg caataccgta atgaaaggca
     5341 acgtgaaaaa ggtccgccgt ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta
     5401 ctgcggatgc ttttacgctg ttcgaactgt ttgaacgtaa actggaaatg catcagggcc
     5461 atctggttaa agccgccgtt gagctggcaa aagactggcg taccgatcgc atgctgcgca
     5521 aacttgaagc actgctggca gtcgcggatg aaactgcatc gcttatcatc accggtaacg
     5581 gtgacgtggt gcagccagaa aacgatctta ttgctatcgg ctccggcggc ccttacgccc
     5641 aggctgcggc gcgcgcgctg ttagaaaaca ctgaacttag cgcccgtgaa attgctgaaa
     5701 aggcgttgga tattgcaggc gacatttgca tctataccaa ccatttccac accatcgaag
     5761 aattaagcta caaagcgtaa ggatctccca tgtctgaaat gaccccacgc gaaatcgtca
     5821 gcgaactgga taagcacatc atcggccagg acaacgccaa gcgttctgtg gcgattgctc
     5881 tgcgtaaccg ctggcgtcgc atgcagctca acgaagagct gcgccatgaa gtgaccccga
     5941 aaaatatcct gatgatcggc ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg
     6001 ctaagctggc gaatgcgccg ttcatcaaag ttgaagcgac caaattcacc gaagtgggct
     6061 atgtcggtaa ggaagtggat tctattattc gcgatctgac cgatgccgcc gtgaaaatgg
     6121 tacgcgtcca ggctatcgag aaaaaccgtt atcgcgctga agaactggca gaagaacgta
     6181 ttctcgacgt gctgatccca cctgctaaaa acaactgggg acagaccgaa cagcagcagg
     6241 aaccgtccgc tgctcgtcag gcattccgca aaaaactgcg tgaaggccag cttgatgaca
     6301 aagaaatcga gatcgatctt gccgcagcac cgatgggcgt tgaaattatg gctcctccag
     6361 gcatggaaga gatgaccagc cagctgcagt ccatgttcca gaacctgggc ggccagaagc
     6421 aaaaagcgcg taagctgaaa atcaaagacg ccatgaagct gctgattgaa gaagaagcgg
     6481 cgaaactggt gaacccggaa gagctgaagc aagacgctat cgacgctgtt gagcagcacg
     6541 ggatcgtgtt tatcgacgaa atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg
     6601 atgtttctcg tgaaggcgtt cagcgtgacc tgctgccgct ggtagaaggt tgcaccgttt
     6661 ccaccaaaca cgggatggtc aaaactgacc acattctgtt tatcgcttct ggcgcgttcc
     6721 agattgcgaa accgtctgac ctaatcccgg aactgcaagg tcgtctgcca atccgcgttg
     6781 aactgcaggc actgaccacc agcgacttcg agcgtattct gaccgagccg aatgcctcta
     6841 tcaccgtgca gtacaaagca ctgatggcga ctgaaggcgt aaatatcgag tttaccgact
     6901 ccggtattaa acgcatcgcg gaagcggcat ggcaggtgaa cgaatctacc gaaaacatcg
     6961 gtgctcgtcg tttacacact gttctggagc gtttaatgga agagatttcc tacgacgcca
     7021 gcgatttaag cggtcaaaat atcactattg acgcagatta tgtgagcaaa catctggatg
     7081 cgttggtggc agatgaagat ctgagccgtt ttatcctata atcgcgttca atcattttca
     7141 tcattgtttg atggggctga aaggccccat ttttattggc gcgtattatg actgaacaac
     7201 aaattagccg aactcaggcg tggctggaaa gtttacgacc taaaaccctc cccctcgcct
     7261 ttgctgcaat tatcgtcggg acggcgctgg catggtggca aggtcatttt gatccgctgg
     7321 tcgccctgct ggcactgatt accgccgggc tattacagat cctttctaac ctcgccaatg
     7381 attacggcga tgcggtaaaa ggcagcgata gacctgaccg cattgggccg ctacgcggca
     7441 tgcaaaaagg ggtcattacc cagcaagaga tgaaacgggc gctcattatt accgtcgtgc
     7501 tcatctgtct ctccgggctg gcactggttg cagtggcgtg ccatacgctg gctgattttg
     7561 tcggtttcct gattcttggc gggttgtcga tcattgccgc tatcacctac accgtgggca
     7621 atcgtcctta tggttatatc ggtctggggg atatttccgt actggttttc tttggctggt
     7681 taagcgtcat ggggagctgg tatttacagg ctcatacatt gattccggca ctgatccttc
     7741 cggcgaccgc atgcggcctg ctggcaacgg cagtactgaa cattaataac ctgcgtgata
     7801 tcaatagcga ccgagaaaac ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg
     7861 cgcgtcgtta tcatgcctgc ctgctgatgg gctctctggt atgtctggcg ctgtttaatc
     7921 tcttttcgct gcatagcctg tggggctggc tgttcctgct ggcggcaccg ttgctggtga
     7981 agcaagcccg ttatgtgatg cgggaaatgg acccggtggc gatgcgaccg atgctggaac
     8041 gcactgtcaa gggggcgtta ctgactaacc tgctgtttat tttagggata ttcctaagcc
     8101 agtgggcagc ataactgaca aatatcaatt aacaattgat gattttgcca acagcccaca
     8161 tagcgcgata tactgaaaat tctcgcagca actgaatgtt aagcctatga aatacgatac
     8221 ttccgagctt tgtgacatct atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa
     8281 ctttggcgga cgggcgtcgt ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa
     8341 cgggttgctg tacgatctgc tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg
     8401 cggtggttct gttcgtcgcg cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa
     8461 tgaatgggaa ggtctggtta tttacggcgc ggtgcgtcag gtagatgacc tggaagagtt
     8521 ggatatcggc atccaggcga tggcggcaat tccggttggt gccgctggcg aaggcattgg
     8581 cgaaagcgat gtccgcgtca attttggcgg tgtaaccttc ttctccggcg accatcttta
     8641 tgccgacaat accgggatta ttctttcaga agatccgctg gatattgaat gataagaaag
     8701 gcaccgcaag gtgccttttt tctgcgttac ctgttggcct acacagtaaa gaaattacgc
     8761 gaaagatgaa gcgtaatcag acctcttcca tgcgacccag cagggcctgc agacgttcct
     8821 gccagccgtt ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat
     8881 gctgggcatt ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt
     8941 ccatctgcaa cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa
     9001 acacttctaa tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaaat
     9061 cctcgtcccg attaccggtg acgccttaat aaatacgagc gcactttagt tagctccgat
     9121 tgtatgaagc cgcgccatcg ctgtccagcg gcacgccttg cagattacgg tttgccacac
     9181 ttttcatcct tctcctggtg acataatcca catcaatcga aaatgttaat aaatttgttg
     9241 cgcgaatgat ctaacaaaca tgcatcatgt acaatcagat ggaataaatg gcgcgataac
     9301 gctcatttta tgacgaagca cacacatttt aagttcgata tttctcgttt ttgctcgtta
     9361 acgataagtt tacagcatgc ctacaagcat cgtggaggtc cgtgactttc acgcatacaa
     9421 caaacattaa ctcttcagga tccgattatg agtcaaacat caaccttgaa aggccagtgc
     9481 attgctgaat tcctcggtac cgggttgttg atttttttcg gtgtgggttg cgttgcagca
     9541 ctaaaagtcg ctggtgcgtc ttttggtcag tgggaaatca gtgtcatttg gggactgggg
     9601 gtggcaatgg ctatctacct gaccgcaggg gtttccggcg cgcatcttaa tcccgctgtt
     9661 accattgcat tgtggctgtt tgcctgtttc gacaagcgca aagttattcc ttttatcgtt
     9721 tcacaagttg ccggcgcttt ctgtgctgcg gctttagttt acgggcttta ctacaattta
     9781 tttttcgact tcgagcagac tcatcacatt gttcgcggca gcgttgaaag tgttgatctg
     9841 gcaggcactt tctctactta ccctaatcct catatcaatt ttgtgcaggc tttcgcagtt
     9901 gagatggtga ttaccgctat tctgatgggg ctgatcctgg cgttaacgga cgatggcaac
     9961 ggtgtaccac gcggcccttt ggctcccttg ctgattggtc tactgattgc ggtcattggc
    10021 gcatctatgg gcccattgac gggttttgcc atgaacccag cgcgtgactt cggtccgaaa
    10081 gtctttgcct ggctggcggg ctggggcaat gtcgccttta ccggcggcag agacattcct
    10141 tacttcctgg tgccgctttt tggccctatc gttggcgcga ttgtaggtgc atttgcctac
    10201 cgcaaactga ttggtcgcca tttgccttgc gatatctgtg ttgtgaaaga aaaggaaacc
    10261 acaactcctt cagaacaaaa agcttcgctg taatatgact acgggacaat taaacatgac
    10321 tgaaaaaaaa tatatcgttg cgctcgacca gggcaccacc agctcccgcg cggtcgtaat
    10381 ggatcacgat gccaatatca ttagcgtgtc gcagcgcgaa tttgagcaaa tctacccaaa
    10441 accaggttgg gtagaacacg acccaatgga aatctgggcc acccaaagct ccacgctggt
    10501 agaagtgctg gcgaaagccg atatcagttc cgatcaaatt gcagctatcg gtattacgaa
    10561 ccagcgtgaa accactattg tctgggaaaa agaaaccggc aagcctatct ataacgccat
    10621 tgtctggcag tgccgtcgta ccgcagaaat ctgcgagcat ttaaaacgtg acggtttaga
    10681 agattatatc cgcagcaata ccggtctggt gattgacccg tacttttctg gcaccaaagt
    10741 gaagtggatc ctcgaccatg tggaaggctc tcgcgagcgt gcacgtcgta gtgaattgct
    10801 gtttggtacg gttgatacgt ggcttatctg gaaaatgact cagggccgtg tccatgtgac
    10861 cgattacacc aacgcctctc gtaccatgtt gttcaacatc cataccctgg actgggacga
    10921 caaaatgctg gaagtgctgg atattccgcg cgagatgctg ccagaagtgc gtcgttcttc
    10981 cgaagtatac ggtcagacta acattggcgg caaaggcggc acgcgtattc caatctccgg
    11041 gatcgccggt gaccagcagg ccgcgctgtt tggtcagttg tgcgtgaaag aagggatggc
    11101 gaagaacacc tatggcactg gctgctttat gctgatgaac actggcgaga aagcggtgaa
    11161 atcagaaaac ggcctgctga ccaccatcgc ctgcggcccg actggcgaag tgaactatgc
    11221 gttggaaggt gcggtgttta tggcaggcgc atccattcag tggctgcgcg atgagatgaa
    11281 gttgattaac gacgcctacg attccgaata tttcgccacc aaagtgcaaa acaccaatgg
    11341 tgtgtatgtg gttccggcat ttaccgggct gggtgcgccg tactgggacc cgtatgcgcg
    11401 cggggcgatt ttcggtctga ctcgtggggt gaacgctaac cacattatac gcgcgacgct
    11461 ggagtctatt gcttatcaga cgcgtgacgt gctggaagcg atgcaggccg actctggtat
    11521 ccgtctgcac gccctgcgcg tggatggtgg cgcagtagca aacaatttcc tgatgcagtt
    11581 ccagtccgat attctcggca cccgtgttga gcgcccggaa gtgcgcgaag tcaccgcatt
    11641 gggtgcggcc tatctcgcag gcctggcggt tggcttctgg cagaacctcg acgagctgca
    11701 agagaaagcg gtgattgagc gcgagttccg tccaggcatc gaaaccactg agcgtaatta
    11761 ccgttacgca ggctggaaaa aagcggtcaa acgcgcgatg gcgtgggaag aacacgacga
    11821 gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcctgaa acgtgcgggg
    11881 gcaaccccgc acacatcaat aatcccaccc ttcccctgtg ctacacttcg cgccattcct
    11941 tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg cgtcaccgaa
    12001 tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac cgcggacggc
    12061 gcggcggtaa acgccatgcg tattatgctc aaccaggtca acattgacgg caccatcgtc
    12121 attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa agtcggtact
    12181 ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg catgacggcg
    12241 atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg cttcctcaat
    12301 gcgccggata tgtatatgga gaagctgatt gtcgggccgg gagccaaagg caccattgat
    12361 ctgaacctgc cgctggcgga taacctgcgc aatgtagcgg cggcgctcgg caaaccgttg
    12421 agcgaactga cggtaacgat tctagctaaa ccacgccacg atgccgttat cgctgaaatg
    12481 cagcaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc ctcaattctc
    12541 acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc gccggaaggc
    12601 gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg tctgctggcg
    12661 cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca ggagctggca
    12721 cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga tatggcgcgc
    12781 agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct ggaaggcatt
    12841 agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa gtcacgcacc
    12901 attcgccgca ttcagtccat ccactatctg gatcgcaaag acccggaaat gcaggtgcac
    12961 atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga atttttttgt
    13021 tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc tgattgggta
    13081 acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct caccgttcac
    13141 gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga aatcgacggc
    13201 gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga tctggagttt
    13261 tacctggtca ccgtccccga tggcaaatta agcccacgat tggcggcact gaaaccaggc
    13321 gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggatga agtaccagat
    13381 tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt atcgattctg
    13441 caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc cgcacgttat
    13501 gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta cgaaggcaaa
    13561 ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac cggacggata
    13621 ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat gaataaagaa
    13681 accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca acagttgctg
    13741 aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat gacagcggag
    13801 cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc gcactcatct
    13861 tattgatcat cccgcgatat gctggcatca aacggttgat aaatgagtga tgatgaccac
    13921 agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc ggcatttctc
    13981 cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt ttatacagct
    14041 tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg aagtagatac
    14101 ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga gcgtcattat
    14161 gtctggcgag ctccagagct ttattcacca gtaaggcatc ttcttcattc ccggaaattg
    14221 ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc atcaagctaa
    14281 cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag tgaaggccgc
    14341 atcaagctca acttgcccct ccagatcctt aatcttgtag gccacgctgg ccccttcatc
    14401 tacagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact ctgcttcttc
    14461 gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa tcaccgtacc
    14521 gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca tagggcacct
    14581 ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta agggaagaac
    14641 aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg tgaagagttc
    14701 agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg gaaaaaaata
    14761 gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta tacttcacat
    14821 cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca ccaagatcga
    14881 taagcatcat caccagaatc aacgtcggga caaaacaccc caccgcccat tgccagacac
    14941 ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc agaaatgccc
    15001 atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct gttggccaaa
    15061 gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta cccgccagtg
    15121 agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa tcacggcgtc
    15181 caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt ccttgatggt
    15241 tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt aatcggagcc
    15301 agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt ctcttatgtt
    15361 ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca acgaccgcgc
    15421 cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt cgagaagact
    15481 ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac agcagtcccg
    15541 acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat gcttctacag
    15601 cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta cccatccagg
    15661 ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca gtgatccgca
    15721 cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg ctgctaacgg
    15781 cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat gttgtcgcgg
    15841 acaacggcga aaaggggctg accttcgctg ttgaaccgat taagctggcg ctatctgaat
    15901 cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga atctctatac
    15961 tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct tataagcgtg
    16021 gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca
    16081 catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg ctggctgtgc
    16141 ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag ctgaaggcag
    16201 ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat tcaccggtga
    16261 aacctctgct gctatgctga aagacatcgg cgcacagtac atcatcatcg gccactctga
    16321 acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg cggtgctgaa
    16381 agagcagggt ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa acgaagcggg
    16441 caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc agggtgctgc
    16501 ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta ctggcaaatc
    16561 tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca tcgctaaagt
    16621 tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa acgcgtctaa
    16681 cgctgcagaa ctgtttgctc agccagacat cgacggcgcg ctggttggcg gtgcgtctct
    16741 gaaagctgac gctttcgcag taatcgttaa agctgcagaa gcggctaaac aggcttaagt
    16801 ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg ccttaacgca
    16861 aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc gtcagtagat
    16921 ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc gccagcccgt
    16981 agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata aatgggctgc
    17041 gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgcccg cgcaaaccac
    17101 ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt tgttcgcgca
    17161 catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc ccggtgcggg
    17221 agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtacttt ttgctcataa
    17281 aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg gtcaacaaag
    17341 gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt gggccattaa
    17401 gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc gaaggatttt
    17461 gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat gtatccgact
    17521 cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata actatcatca
    17581 ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa gagatgcaag
    17641 ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg ggtttgataa
    17701 ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt agcaaaatgc
    17761 tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa cttcagcttt
    17821 ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta gtagtttttc
    17881 gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc cgccacctct
    17941 ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc tgcgaggata
    18001 gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc tgccagcaga
    18061 gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa ggtgttagtg
    18121 gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg aacaaaatcc
    18181 tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc agccaggtag
    18241 ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac acccggttta
    18301 atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag gaaaacaatg
    18361 gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt atcaatccgc
    18421 ccgcgttccg caattgcgtc cacgtcatag gccagagcca gcgtgacaac atcagcttca
    18481 ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg acgcatcacc
    18541 acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta ctgttcgtac
    18601 aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc cataacgctg
    18661 gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg actctcttat
    18721 gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag aataaaaaaa
    18781 gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga aatccgcctc
    18841 atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc ttatccggcc
    18901 tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata agcgaagcgc
    18961 atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct ttttccgaaa
    19021 tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac gcttcatgtt
    19081 ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac ctacacaacg
    19141 accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg aagccagaat
    19201 acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag ttgcgcgggt
    19261 ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac acatatgttc
    19321 ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga tttcgtttac
    19381 caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc caccggcaat
    19441 ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca cggaaatacg
    19501 ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg tgctcagcgc
    19561 agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag tgcccggcag
    19621 accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg aaccgtcacc
    19681 gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag ccacggcgcg
    19741 gatgttctcg tcgcggaatt ccgggaaacg cgcagaaccg aggaacgtac cgccacggtt
    19801 gatcatgtca gacacgctgt aacggtctag ctgtaccata cggtcttcat acagacccag
    19861 atagccgtca taaataccca ttacttccag accttctgtc agcgcagaac gaacaacccc
    19921 gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac cgattttctt
    19981 aatcatgact acctctgaac tttggaatgc aaaatgaaat ctgttgccgg aagtcttctt
    20041 gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc tgctgaattg
    20101 attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg ctctctttta
    20161 tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac aggggtcctg
    20221 atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta cctgatccgc
    20281 caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa tctgaataaa
    20341 gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac ccggccagga
    20401 agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat ccagtaatga
    20461 ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc cgattcccaa
    20521 tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg ccagcagaat
    20581 tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca ccgcctggct
    20641 ttgcgtccgg cgcaccaccc aacgctgaaa cgagacaagg ataatcgtac aaattagcgc
    20701 cacaattgtc acgataaccc cgacgcctgg atctatcatc ggtgttggag atatcagatg
    20761 ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac tttgcgccag
    20821 cgccgcgagg gactctgctt taccgtgacc aaacgagtga ttatcgtcgg caggttgcag
    20881 ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca gcgaatccac
    20941 cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt taatcagcaa
    21001 tagcaacgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc gtccataaga
    21061 ttgattcata aatactcccg ctatcaactg acgctagtat aacggaagca aatcatctgc
    21121 aatgcattaa gcagcaggca aattgaggat aaaaaaaacc ccacatcatg tgggggaaga
    21181 cagggatggt gtctatggca aggaaaacag ggtttactac tgggaacgtg agttgctact
    21241 actcaatagc ttcaacgatg aacttttttg ccattgcgtc acgtcacgca actgctccat
    21301 tcgttgttga tgtttctcgt ttaaaaccgc ttgctgctcc ggcgttaaca ggcgatacat
    21361 ttggttgcgg acttttgcca tctcaacctg acgagcaatt tgctcattcg ccattttttc
    21421 tgcctgtgcg cgcacagcgt tttcatcaaa attttctgcg gtgacaaggc gatgcattgt
    21481 ctccagttcg ctaacattaa caggaggctg ttcgtgccgg gcctgttgca taagatctcg
    21541 catctgctga cgctgatgtt cggttaaact tatgccgtcg aacatatggc tctgcgtact
    21601 gcgctgcgta ctgcgctgcg taagttcttc acccggatgc cagttatcgc ctgaaccgac
    21661 ttcagcagcg tggcttaatg aactgactgc cagcgttgaa gccatgacgg cagcggtaac
    21721 tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt caacgataga gagtttacga
    21781 ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt aaagtcatgg attagcgacg
    21841 tctgatgacg taatttctgc ctcggaggta tttaaacaat gaataaaatc ctgttagttg
    21901 atgatgaccg agagctgact tccctattaa aggagctgct cgagatggaa ggcttcaacg
    21961 tgattgttgc ccacgatggg gaacaggcgc ttgatcttct ggacgacagc attgatttac
    22021 ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga cacattaaaa gcacttcgcc
    22081 agacacacca gacgcctgtc attatgttga cggcgcgcgg cagtgaactt gatcgcgttc
    22141 tcggccttga gctgggcgca gatgactatc tcccgaaacc gtttaatgat cgtgagctgg
    22201 tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag cgagcaacag caaaacaacg
    22261 acaacggttc accgacactg gaagttgatg ccttagtgct gaatccaggc cgtcaggaag
    22321 ccagcttcga cgggcaaacg ctggagttaa ccggtactga gtttaccctg ctctatttgc
    22381 tggcacagca tctgggtcag gtggtttccc gtgaacattt aagccaggaa gtgttgggca
    22441 aacgcctgac gcctttcgac cgcgctattg atatgcacat ttccaacctg cgtcgtaaac
    22501 tgccggatcg taaagatggt cacccgtggt ttaaaacctt gcgtggtcgc ggctatctga
    22561 tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc ttcgccatct tctggctgac
    22621 gctggcgctg gtgttgatgt tggttttgat gttacccaag ctcgattcac gccagatgac
    22681 cgagcttctg gatagcgaac agcgtcaggg gctgatgatt gagcagcatg tcgaagcgga
    22741 gctggcgaac gatccgccca acgatttaat gtggtggcgg cgtctgttcc gggcgattga
    22801 taagtgggca ccgccaggac agcgtttgtt attggtgacc accgaaggcc gcgtgatcgg
    22861 cgctgaacgc agcgaaatgc agatcattcg taactttatt ggtcaggccg ataacgccga
    22921 tcatccgcag aagaaaaagt atggccgcgt ggaactggtc ggtccgttct ccgtgcgtga
    22981 tggcgaagat aattaccaac tttatctgat tcgtccggcc agcagttctc aatccgattt
    23041 cattaactta ctgtttgacc gcccgctatt actgctgatt gtcaccatgt tggtcagtac
    23101 gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg gcgcgtaagc tgaaaaacgc
    23161 tgccgatgaa gttgcccagg gaaacttacg ccagcacccg gaactggaag cggggccaca
    23221 ggaattcctt gccgcaggtg ccagttttaa ccagatggtc accgcgctgg agcgcatgat
    23281 gacctctcag cagcgtctgc tttctgatat ctctcacgag ctgcgcaccc cgctgacgcg
    23341 tctgcaactg ggtacggcat tactgcgccg tcgtagcggt gaaagcaagg aactggagcg
    23401 tattgaaacc gaagcgcaac gtctggacag catgatcaac gatctgttgg tgatgtcacg
    23461 taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa gccaaccagt tgtggagtga
    23521 agtgctggat aacgcggcgt tcgaagccga gcaaatgggc aagtcgttga cagttaactt
    23581 cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc ctggaaagtg cgctggaaaa
    23641 cattgttcgt aatgctctgc gttattccca tacgaagatt gaagtgggct ttgcggtaga
    23701 taaagacggt atcaccatta cggtggacga cgatggtcct ggcgttagcc cggaagatcg
    23761 cgaacagatt ttccgtccgt tctatcggac cgatgaagca cgcgatcgtg aatctggcgg
    23821 tacaggattg gggctggcga ttgttgaaac cgccattcag cagcatcgtg gctgggtgaa
    23881 ggcagaagac agcccgctgg gcggtttacg gctggtgatt tggttgccgc tgtataagcg
    23941 gagttaaact ccgcatttgt aggcaggata aggcgtttac gccgcatccg gcatttgagc
    24001 aggatgcctg atgcgacgct gatagcgtct tatcaggcct acactccggg ggttattttc
    24061 cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag tcggcgcttt tgcatcgtcc
    24121 gcgtccagct tttcgataac ccggcggcgg agagtaaacg gtgatactga tcgtcatcaa
    24181 acggcatatg ccatgcgatg gcggcagcct cctgcacggt cacatcgctg acacgggaaa
    24241 ccaactccag cggcgcgtcc gcagacactt tccctggcgc tatcacgcta tacaaccacc
    24301 ccaccttacc tgtgttttgc atcaactgcg ctatatcgct gatatcaaaa tggtaattga
    24361 gtttatagca aggcgagcgc ggctggctga cctgaattaa tgcctctccc cagcggaaaa
    24421 tatcgcccat ataaacatta ctttccgtca ggccgtcggt tgagaggttt tcaccaaacg
    24481 caggcgcaac aaacaactcc gcctgttccg gaaattcccg cgcccagtag agataatgct
    24541 cacgaggata atgacacagc gctctgtctg gcccaccatg aacctttttc tccgcctgct
    24601 cgtcaccttc cagccccagc tccgtcagca tcaactcacc atcaacctgg attttggcaa
    24661 ttgcgctggg tttgccttcg ggataagcct gaatcttgcc tgtgtataca tcaaccggat
    24721 atcgcatctg ttccctctcc acgagaaatt tccccattag accatattta ttttatgtca
    24781 ggcgtgctgt tttcgcggta agtatccagg caggaactca atagcaaacc ggacgaacgt
    24841 tagttcatcc ggctgtgggg attaaggaag caggtttatt tcaccgtacc taaaatttcg
    24901 attttcgctg ctctggcttt gactttacgt gaccagatag aggtaagaat tggcaccaga
    24961 atcgaagtca caatgaccgc cgtcgctacc agcgaagttg ctgccggagc catcggttta
    25021 aacgcaggca ccatttctgc aatcagcata ggtgtcgcta ccgcggcccc tgcggaactg
    25081 gaagcggcaa ttccggcagt gccatcgcca ccgccaatca atttatcggc gataatcaac
    25141 ggaataccgg tcacgataat tactgccaca cccaacagga tccccagcaa accagtctgg
    25201 gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt
    25261 tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa ggttccccag ggcaaagccc
    25321 accaggaacg gtaatactgc gccgacgaaa acatgcggtt caaacgaggc aatccctgca
    25381 gtgcccagaa taatcatcgt catgagcggc ccggactcca acgacatcaa cacaaatgcc
    25441 ccagcttctt cttttgtgcc gtactgctgc atgatggaag cgtaaagtcc gccattggtc
    25501 atatccatcg ccgccaccag cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca
    25561 ccatgttccg gaataatgcg cgaggcaatt gccgcaacca cccacgcgac ggcaattttt
    25621 gttaccacca gcgtaccgga tttacgcaat accgttcccg tcgcgcttaa ttttattgac
    25681 gcccccatgc aaaaaaacca caccgccaga atggacaccg taccggtaat catcccgttg
    25741 gtgaatgatc caaaatattt ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg
    25801 aatagcggga cgagcatcat ccccccccgg gattttctca atcgagcgtt ttatctgcat
    25861 ttccatcacc tgtcggcata tttttaaggg ctgatgcgta attcctcaat ggagcatcag
    25921 tgttgatgga aacgaagata gacattcatt taatcaataa aagtgatcaa aaccacatca
    25981 attgaaacgc tgttttattt ttatcggatc attgttttta ttttgaaatt gatcacaaaa
    26041 aaaccaccgc cgttggcgat ggttcatttt ttaagctgat atgcggcgta aacgcctcat
    26101 tgcagcaggc ggcaaatgat tattttttcg ccgcgaaacg tgccgctgct tcatcccagt
    26161 tcaccacgtt ccagaactct ttgatgtagt ccggacggcg gttctggaat ttcaggtagt
    26221 aagcatgttc ccacacatcc aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt
    26281 cacccatcag cggggaatcc tggttagcag tagaaaccac cgccagttta tcgcctttca
    26341 gcaccagcca tgcccagccg gaaccaaagc gggaagctgc cgctttctca aattctgctt
    26401 tgaagttatc aacggagccg aagtcacgtt cgatagccgc tttcaggtca ccctgtaagg
    26461 tggtgccttt tttcagacct ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt
    26521 tgcgcagtac ggttttcttg tctgctggca gctggtccag tttggtgatc agctcttcaa
    26581 ccggcaggtt ggcaaattct ggcaggcttt ccagcgccgc gttggcgttg ttgacgtagg
    26641 tctgatggtg tttggtgtgg tggatttcca tggtctgctt atcgaagtgc ggttccaggg
    26701 catcgtaagc atacggcagg gatggcaggg tatagctcat attcatctcc agtattgtcg
    26761 ggcggccgat tgttaatgcg cgtaagcagt tggttcatta tagttaatta aatgatattg
    26821 aaaatgatta tcaatgccgt acttttcgta agggtatggt tttgcaggaa aattcccgag
    26881 atgtgaagca aatcacccac ttaatgccgt gattgccagt aaatcgacaa cggcggcaac
    26941 aggcgaaagg ttaatcgaca gcacgatttt tacactcatc tcgtcggaga tgtgacgcga
    27001 cgaaaaatga tgaggataag aagatgagta acgcgattac gatggggata ttttggcatt
    27061 tgatcggcgc ggccagtgca gcctgttttt acgctccgtt caaaaaagta aaaaaatggt
    27121 catgggaaac catgtggtca gttggtggga ttgtttcgtg gattattctg ccgtgggcca
    27181 tcagcgccct gttactgccg aatttctggg cgtattacag ctcgtttagt ctctctacgc
    27241 tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg taatatcaac tacggcctga
    27301 ccatgcgtta tctcggcatg tcgatgggaa ttggcatcgc cattggcatt acgttgattg
    27361 tcggtacgct gatgacgcca attatcaacg gcaatttcga tgttctgctt aataccgaag
    27421 gcggacgcat gacgttgctc ggcgttctgg tggcgctgat tggtgtaggg attgtgactc
    27481 gcgccgggca gttgaaagag cgcaaaatgg gcattaaagc cgaagagttc aacctgaaaa
    27541 aagggctggt gctggcggtg atgtgcggca ttttctctgc cggtatgtct tttgcgatga
    27601 acgccgcaaa accgatgcat gaagccgctg ccgcactggg cgtcgatcca ctgtatgtcg
    27661 ctctgccaag ctatgtcatc atcatgggcg gcggcgcgat catcaacctc ggtttctgct
    27721 tcattcgtct ggcaaaagtg aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac
    27781 cgctgatcat tcacaatgtg ttactctcag cactgggcgg gttgatgtgg tatctgcaat
    27841 tctttttcta tgcctggggc cacgcccgca ttccggcgca gtatgactac atcagttgga
    27901 tgctgcatat gagtttctat gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt
    27961 ggaacaatgc aggtcgccgt ccggtaacgg tgttgagcct cggttgtgtg gtgattattg
    28021 tcgccgccaa catcgtcggc atcggcatgg cgaattaatc tttctgcgaa ttgagatgac
    28081 gccactggct gggcgtcatc ccggtttccc gggtaaacac caccgaaaaa tagttactat
    28141 cttcaaagcc acattcggtc gaaatatcac tgattaacag gcggctatgc tggagaagat
    28201 attgcgcatg acacactctg acctgccgca gatattgatt gatggtcatt ccagtctgct
    28261 ggcgaaattg ctgacgcaaa acgcgctcac tgcacgatgc ctcatcacaa aatttatcca
    28321 gcgcaaaggg acttttcagg ctagccgcca gccgggtaat cagcttatcc agcaacgttt
    28381 cgctggatgt tggcggcaac gaatcactgg tgtaacgatg gcgattcagc aacatcacca
    28441 actgcccgaa cagcaactca gccatttcgt tagcaaacga cacatgctga ctactttcat
    28501 gctcaagctg accgataacc tgccgcgcct gcgccatccc cacgctacct aagcgccagt
    28561 gtggttgccc tgcgctggcg ctaaatcccg gaatcgctcc ctgccagtca agattcagct
    28621 tcagccgctc cgggcaataa ataatattct gcaaaaccag atcgttaacg gaagcgtagg
    28681 agtgtttatc gtcagcatga atgtaaaaga gatcgccacg ggtaatgcga taagggcgat
    28741 cgttgagtac atgcagacca ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg
    28801 tatgttcggc aaaaacatct tgcggataac ggtcagccac agcgactgcc tgctggtcgc
    28861 tggcaaaaaa atcatctttg agaagtttta actgatgcgc caccgtggct acctcggcca
    28921 gagaacgaag ttgattattc gcaatatggc gtacaaatac gttgagaaga ttcgcgttat
    28981 tgcagaaagc catcccgtcc ctggcgaata tcacgcggtg accagttaaa ctctcggcga
    29041 aaaagcgtcg aaaagtggtt actgtcgctg aatccgcagc gataggcgat gtcagtaacg
    29101 ctggcctcgc tgtgacgtag cagatgtcgg gccttcatca gtcgcaggcg gttcaggtaa
    29161 cgctgaggcg tcagtcccgt ttgctgctta agctgccgat gtagcgtacg cagtgaaaga
    29221 gaaaattgat ccgccacggc atcccaattc acctcatcgg caaaatggtc ctccagccag
    29281 gccagaagca agttgagacg tgatgcgctg ttttccaggt tctcctgcaa actgctttta
    29341 cgcagcaaga gcagtaattg cataaacaag atctcgcgac tggcggtcga gggtaaatca
    29401 ttttcccctt cctgctgttc catctgtgca accagctgtc gcacctgctg caatacgctg
    29461 tggttaacgc gccagtgaga cggatactgc ccatccagtt cttgtggcag caactgattc
    29521 agcccggcga gaaactgaaa tcgatccggc gagcgataca gcacattggt cagacacaga
    29581 ttatcggtat gttcatacag atgccgatca tgatcgcgta cgaaacagac cgtgccaccg
    29641 gtgatggtat agggctgccc attaaacaca tgaatacccg tgccatgttc gacaatcaca
    29701 atttcatgaa aatcatgatg atgttcagga aaatccgcct gcgggagccg gggttctatc
    29761 gccacggacg cgttaccaga cggaaaaaaa tccacactat gtaatacggt catactggcc
    29821 tcctgatgtc gtcaacacgg cgaaatagta atcacgaggt caggttctta ccttaaattt
    29881 tcgacggaaa accacgtaaa aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa
    29941 atgcggtgag catcacatca ccacaatgaa gcaaattgtg aacatcatca cgttcatctt
    30001 tccctggttg ccaatggccc attttcctgt cagtaacgag aaggtcacga attcaggcgc
    30061 tttttagact ggtcgtaatg aaattcagca ggatcacatt atgacctttc gcaattgtgt
    30121 cgccgtcgat ctcggcgcat ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg
    30181 ccgcagcctg acgctgcgcg aaatccatcg ttttaacaat ggcctgcata gccagaacgg
    30241 ttatgtcacc tggaatgtgg atagcctgga aagtgccatt cgccttggat taaacaaggt
    30301 gtgcgaggaa gggattcgta tcgatagcat tgggcttgat acctggggcg tggactttgt
    30361 gctgctcgac caacagggtc agcgtgtggg cctgcccgtt gcttatcgcg atagccgctc
    30421 caatggccta atggcgcagg cacagcaaca actcggcaaa cgcgatattt atcaacgtag
    30481 cggcatccag tttctgccct tcaatacgct ttatcaactg cgtgcgctga cggagcaaca
    30541 acctgaactt attccacaca ttgctcacgc tctgctgatg ccggattact tcagctatcg
    30601 cctgaccggc aagatgaact gggaatatac caatgccacc accacacaac tggtcaatat
    30661 caatagcgac gactgggacg agtcgctact ggcgtggagc ggggccaaca aagcctggtt
    30721 tggtcgcccg acgcatccgg gtaatgtcat tgggcactgg atttgcccgc agggtaatga
    30781 gattccggtg gtcgccgttg ccagccatga taccgccagc gcggttatcg cctcgccgtt
    30841 aaacggttca cgcgctgctt atctctcttc tggcacctgg tcattgatgg gcttcgaaag
    30901 ccagacgcca tttaccaatg acacggcgct ggcagccaac ataaccaatg aaggcggggc
    30961 ggaaggtcgc tatcgggtgc tgaaaaatat tatgggctta tggctgcttc agcgagtgct
    31021 tcaggagcgg caaatcaacg atctcccggc gcttatcgcc gcgacacagg cacttccggc
    31081 ctgccgcttc atcatcaatc ccaatgacga tcgctttatt aatcctgagg cgatgtgcag
    31141 cgaaattcag gctgcgtgtc gggaaacggc gcaaccgatc ccggaaagtg atgctgaact
    31201 ggcgcagcta cgcggtgaag atttctcgca actgcatatt gtcggcggcg gctgccagaa
    31261 cacgctgctc aaccagctat gtgccgatgc ctgcggtatt cgggtgatcg ccgggcctgt
    31321 tgaagcctcg acgctcggca atatcggcat ccagttaatg acgctggatg aactcaacaa
    31381 tgtggatgat ttccgtcagg tcgtcagcac caccgcgaat ctgaccacct ttacccctaa
    31441 tcctgacagt gaaattgccc actatgtggc gcagcttcac tctacacgac agacaaagga
    31501 gctttgcgca tgaccactca actggaacag gcctgggagc tggcgaaaca gcgtttcgcg
    31561 gcggtgggga ttgatgtcga ggaggcgctg cgccaacttg atcgtttacc cgtttcaatg
    31621 cactgctggc agggcgatga tgtttccggt tttgaaaacc cggaaggttc gctgaccggg
    31681 gggattcagg ccacaggcaa ttatccgggc aaagcgcgta atgccagtga gctacgtgcc
    31741 gatctggaac aggctatgcg gctgattccg gggccgaaac ggcttaattt acatgccatc
    31801 tatctggaat cagatacgcc agtctcgcgc gaccagatca aaccagagca cttcaaaaac
    31861 tggggtgaat gggcgaaagc caatcagctc ggtctggatt ttaacccctc ctgcttttcg
    31921 catccgctaa gcgccgatgg ctttacgctc tcgcatcccg acgacagcat tcgccagttc
    31981 tggattgatc actgcaaagc cagccgtcgc gtttcggcct attttggcga gcaactcggc
    32041 acaccatcgg tgatgaacat ctggatcccg gatggtatga aagatatcac cgttgaccgt
    32101 ctcgccccgc gtcagcgtct gctggcagca ctggatgagg tgatcagcaa gaagctaaac
    32161 cctgcgcacc atatcgacgc cgttgagagc aaattgtttg gcattggcgc agagagctac
    32221 acggttggct ccaatgagtt ttacatgggg tatgccacca gccgccagac tgcgctgtgc
    32281 ctagacgccg ggcacttcca cccgactgaa gtgatttccg acaagatttc cgccgccatg
    32341 ctgtatgtgc cgcagttgct gctgcacgtc agccgtccgg ttcgctggga ctgcgatcac
    32401 gtagtgctgc tggatgatga aacccaggca attgccagtg agattgtgcg tcacgatctg
    32461 tttgaccagg tgcatatcgg ccttgacttc ttcgatgcct ctatcaaccg cattgccgcg
    32521 tgggtcattg gtacacgcaa tatgaaaaaa gccctgctgc gtgcgttgct ggaacctacc
    32581 gctgagctgc gcaagctgga agcggcgggc gattacactg cgcgtctggc actgctggaa
    32641 gagcagaaat cgttgccgtg gcaggcggtc tgggaaatgt attgccaacg tcacgatacg
    32701 ccagcaggta gcgaatggct ggagagcgtg cgggcttatg aaaaagaaat tttgagccag
    32761 cgtgggtaaa cactgccgga tgcggcgcga gcgccttatc cggcctacgg gtcggcaaca
    32821 gttgtaggcc tgataagacg cgacagcgtc gcatcaggca ttgattgccg gatgcggcac
    32881 gagtgcctta tccggcctac aggtcggcaa tagttgtagg cctgataaga cgcgacagcg
    32941 tcgcatcagg cattgattgc cggatgcggc acgagcgcct tatccggcct acgggtcgtg
    33001 catctgacaa caccgaattt acaggaacac agaacatgca aaacattacc cagtcctggt
    33061 ttgtccaggg aatgatcaaa gccaccaccg atgcctggct gaaaggctgg gacgaacgca
    33121 acggcggcaa cctgacgctg cgcctggatg acgccgatat cgcgccttat aaagacaacg
    33181 tccatgcaca accgcgctat atcccgctca gccagcccat gcctttactg gcaaatacac
    33241 cgtttattgt caccggctcc ggcaaattct tccgtaacgt ccagctcgat cctgcagcta
    33301 acttaggcgt cgtaaaagtc gacagcgatg gcgcgggcta ccatattctc tgggggttaa
    33361 ccaacgaagc cgtcccgaca tccgaactcc cggcgcactt tctctcccac tgcgagcgca
    33421 ttaaagccac caacggtaaa gatcgggtga tcatgcactg ccacgccacc aacctgatcg
    33481 ccctcaccta tgtactggaa aacgacaccg cggtctttac tcgccaactg tgggaaggca
    33541 gcaccgagtg tctggtggta ttcccggatg gcgttggcat tctgccgtgg atggtgcccg
    33601 gcacggacga aatcggccag gcgaccgcac aagagatgca aaaacattcg ctggtgttgt
    33661 ggcccttcca cggcgtcttc ggcagcgggt cgacgttgga tgaaacattc ggtttaatcg
    33721 acaccgcaga aaaatcagca caagtattag tgaaggttta ttcgatgggc ggcatgaaac
    33781 agaccatcag ccgtgaagag ttgatagcgc tcggcaagcg ctttggcgtt acaccactag
    33841 ccagtgcgct ggcgctgtaa ggagcaaaca tgatccgcaa agcctttgtc atgcaggtta
    33901 accccgacgc ccacgaagag tatcagcgtc ggcataaccc catctggccg gaactggaag
    33961 cagtgttgaa atctcacggt gcgcataact acgccattta cctcgacaaa gcgcgtaatc
    34021 tgctgtttgc cacggtagag attgaatctg aagaacggtg gaatgcggta gccagtgctg
    34081 atgtttgcca acgttggtgg aaatatatga ccgatgttat gcccgctaac ccggataaca
    34141 gcccggtgag tagcgagctg c
//
LOCUS       DAJTSL010000029        32948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTSL010000029 DAJTSL010000000
VERSION     DAJTSL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.029"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..247
                     /locus_tag="OO433_001214"
     CDS             <1..247
                     /locus_tag="OO433_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6049760.1"
                     /translation="CDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNGLSVVMTP
                     GWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            257..1631
                     /gene="gspE"
                     /locus_tag="OO433_001215"
                     /pseudo
     CDS             257..1631
                     /gene="gspE"
                     /locus_tag="OO433_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            1621..2823
                     /gene="hofC"
                     /locus_tag="OO433_001216"
     CDS             1621..2823
                     /gene="hofC"
                     /locus_tag="OO433_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6049761.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(2858..3901)
                     /gene="guaC"
                     /locus_tag="OO433_001217"
     CDS             complement(2858..3901)
                     /gene="guaC"
                     /locus_tag="OO433_001217"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6049762.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSDQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(4057..4101)
                     /locus_tag="OO433_001218"
     CDS             complement(4057..4101)
                     /locus_tag="OO433_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049763.1"
                     /translation="MIIKTLKMSARKRN"
     gene            4126..4746
                     /gene="coaE"
                     /locus_tag="OO433_001219"
     CDS             4126..4746
                     /gene="coaE"
                     /locus_tag="OO433_001219"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6049764.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            4746..5489
                     /gene="zapD"
                     /locus_tag="OO433_001220"
     CDS             4746..5489
                     /gene="zapD"
                     /locus_tag="OO433_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6049765.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            5499..5696
                     /gene="yacG"
                     /locus_tag="OO433_001221"
     CDS             5499..5696
                     /gene="yacG"
                     /locus_tag="OO433_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6049766.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(5803..6192)
                     /gene="mutT"
                     /locus_tag="OO433_001222"
     CDS             complement(5803..6192)
                     /gene="mutT"
                     /locus_tag="OO433_001222"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6049767.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(6252..8957)
                     /gene="secA"
                     /locus_tag="OO433_001223"
     CDS             complement(6252..8957)
                     /gene="secA"
                     /locus_tag="OO433_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6049768.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(9019..9531)
                     /gene="secM"
                     /locus_tag="OO433_001224"
     CDS             complement(9019..9531)
                     /gene="secM"
                     /locus_tag="OO433_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6049769.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQYAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(9762..10679)
                     /gene="lpxC"
                     /locus_tag="OO433_001225"
     CDS             complement(9762..10679)
                     /gene="lpxC"
                     /locus_tag="OO433_001225"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6049770.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(10780..11931)
                     /gene="ftsZ"
                     /locus_tag="OO433_001226"
     CDS             complement(10780..11931)
                     /gene="ftsZ"
                     /locus_tag="OO433_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6049771.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(11992..13254)
                     /gene="ftsA"
                     /locus_tag="OO433_001227"
     CDS             complement(11992..13254)
                     /gene="ftsA"
                     /locus_tag="OO433_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6049772.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(13251..14081)
                     /gene="ftsQ"
                     /locus_tag="OO433_001228"
     CDS             complement(13251..14081)
                     /gene="ftsQ"
                     /locus_tag="OO433_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6049773.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKETAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQLAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(14083..15003)
                     /gene="ddlB"
                     /locus_tag="OO433_001229"
     CDS             complement(14083..15003)
                     /gene="ddlB"
                     /locus_tag="OO433_001229"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6049774.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLDASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(14996..16471)
                     /gene="murC"
                     /locus_tag="OO433_001230"
     CDS             complement(14996..16471)
                     /gene="murC"
                     /locus_tag="OO433_001230"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6049775.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFLGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(16525..17592)
                     /gene="murG"
                     /locus_tag="OO433_001231"
     CDS             complement(16525..17592)
                     /gene="murG"
                     /locus_tag="OO433_001231"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6049776.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(17589..18833)
                     /gene="ftsW"
                     /locus_tag="OO433_001232"
     CDS             complement(17589..18833)
                     /gene="ftsW"
                     /locus_tag="OO433_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6049777.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVVFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(18833..20149)
                     /gene="murD"
                     /locus_tag="OO433_001233"
     CDS             complement(18833..20149)
                     /gene="murD"
                     /locus_tag="OO433_001233"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6049778.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(20152..21234)
                     /gene="mraY"
                     /locus_tag="OO433_001234"
     CDS             complement(20152..21234)
                     /gene="mraY"
                     /locus_tag="OO433_001234"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6049779.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(21228..22586)
                     /gene="murF"
                     /locus_tag="OO433_001235"
     CDS             complement(21228..22586)
                     /gene="murF"
                     /locus_tag="OO433_001235"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6049780.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(22583..24070)
                     /gene="murE"
                     /locus_tag="OO433_001236"
     CDS             complement(22583..24070)
                     /gene="murE"
                     /locus_tag="OO433_001236"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6049781.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKV
                     IDVNYHDSGVTIRFSSSWGDGEIESHLIGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTVPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(24057..25823)
                     /gene="ftsI"
                     /locus_tag="OO433_001237"
     CDS             complement(24057..25823)
                     /gene="ftsI"
                     /locus_tag="OO433_001237"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6049782.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(25839..26204)
                     /gene="ftsL"
                     /locus_tag="OO433_001238"
     CDS             complement(25839..26204)
                     /gene="ftsL"
                     /locus_tag="OO433_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6049783.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(26201..27142)
                     /gene="rsmH"
                     /locus_tag="OO433_001239"
     CDS             complement(26201..27142)
                     /gene="rsmH"
                     /locus_tag="OO433_001239"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6049784.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(27144..27602)
                     /gene="mraZ"
                     /locus_tag="OO433_001240"
     CDS             complement(27144..27602)
                     /gene="mraZ"
                     /locus_tag="OO433_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6049785.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(28204..29208)
                     /gene="cra"
                     /locus_tag="OO433_001241"
     CDS             complement(28204..29208)
                     /gene="cra"
                     /locus_tag="OO433_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6049786.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHLMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(29290..29376)
                     /locus_tag="OO433_001242"
     CDS             complement(29290..29376)
                     /locus_tag="OO433_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6049787.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(29388..29879)
                     /gene="ilvN"
                     /locus_tag="OO433_001243"
     CDS             complement(29388..29879)
                     /gene="ilvN"
                     /locus_tag="OO433_001243"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6049788.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(29882..31606)
                     /gene="ilvI"
                     /locus_tag="OO433_001244"
     CDS             complement(29882..31606)
                     /gene="ilvI"
                     /locus_tag="OO433_001244"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6049789.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVVAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPISISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            31725..31769
                     /locus_tag="OO433_001245"
     CDS             31725..31769
                     /locus_tag="OO433_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049790.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(31924..32868)
                     /gene="leuO"
                     /locus_tag="OO433_001246"
     CDS             complement(31924..32868)
                     /gene="leuO"
                     /locus_tag="OO433_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6049791.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         7908 a         9254 c         8016 g         7770 t
ORIGIN      
        1 ctgcgacggt ggcagcaatg gcattccctc gcctaccacc acccgctatg tttcagccat
       61 gagtgtggca aaaggcgtgg tgtcgctgac cgggcaagaa agtctcaatg gactaagcgt
      121 cgtcatgacg ccaggttggg ataacgcaaa cggcgtcacc ggctgggcgc gcaactgcaa
      181 tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat gtcttccgct ttgatgacgc
      241 caactaagga gcggcaatga atattccaca gctcaccgcc ctgtgtctgc gttatcaggg
      301 agtcttgctg gatgccagcg aagaggtggt tcatgttgcg gtagtcgatg caccttcgca
      361 tgagttgctg gacgcattgc atttcgctac caccaaacgt attgagatca cctgctggac
      421 gcgccaacaa atggaaggcc acgccagtcg cacacaacag acattgcccg tagctgttca
      481 ggagaagcat cagtcgaaag cagagttgct gactcgaacg ttacaatctg cgctggaaca
      541 acgtgcgtct gatattcata tcgaaccagc ggacaatgcc taccgcatcc gcttgcgtat
      601 cgacggcgta ttgcatcctt taccggatgt ttcaccggat gccggagtcg cattaaccgc
      661 cagattaaaa gtgctgggaa acctggatat tgcggaacat cgcctgccgc aggacgggca
      721 attcactgtc gaactggcag gaaacgccgt ctcatttcgt attgcgacct taccatgtcg
      781 gggtggtgaa aaggtggtat taaggttgtt acagcaggtg aaccaggcac tggatgttaa
      841 cacgctggga atgcagccgt tacaactggc ggactttgct catgccttgc aacaaccaca
      901 gggactggtg ctggtaactg gccctacagg cagcggcaaa acggtcacgc tttatagtgc
      961 cctgcaaaag ctgaataccg ctgacattaa tatttgtagc gtcgaagatc cggttgagat
     1021 ccccatagcc ggactaaacc agacgcaaat ccatccgcgt gccgggctca cctttcaggg
     1081 cgttttgcgt gcgttattgc gccaggatcc tgacgtcatc atgatccgat ggcgaaacgg
     1141 cagaaattgc cattaaagcc gcgcaaaccg gtcacctggt gttgtctacc ctacacacta
     1201 attccacctg cgaaacgctg gtacgtttac agcaaatggg agtcgcccgc tggatgctct
     1261 catcagcgct tacgctggta atagcccagc gtctggtacg taaattttgc ccacattgtc
     1321 gccagcagca aggggagccc atccatattc cagacaatgt atggccgtcg ccgctgcccc
     1381 actggcaggc acccggttgt gtacattgct accacggttt ttatggtcgt accgccttgt
     1441 ttgaagtcct gcccattaca ccagccatac gccagcttat ttccgctaat accgacgttg
     1501 aatcgctgga aacgcacgca cgacaggcgg gtatgcgtac gctttttgaa aacggctgcc
     1561 tagccgtgga gcaaggctta accacctttg aagagttaat ccgcgtactg gggatgccgc
     1621 atggcgagta agcaactctg gcgctggcat ggcataaccg gcgacggcaa tgcgcaagat
     1681 gggatgctat gggcagagag ccgtgctttg ctgctcatgg cactacagca acagatggtt
     1741 accccactta gcctgaagcg aatcgccatc aattctgcgc agtggcgagg agataaaagc
     1801 gcggaagtca ttcatcaact ggcgacgcta ctcaaagccg ggttaacgct ttctgaaggg
     1861 ctggcactgc tggcggaaca gcatcccagt aagcaatggc aagcgttgct gcaatcgctg
     1921 gcgcacgatc tcgaacaggg cattgctttt tccaatgcct tattaccctg gtcagaggta
     1981 tttccgccac tctatcaggc gatgatccgc acgggtgaac tgaccggtaa gctggatgaa
     2041 tgctgctttg aactggcgcg tcagcaaaaa gcccagcgtc agttgaccga caaagtgaaa
     2101 tcagcgttac gttatcccat catcatttta gcgatggcaa tcatggtggt tgtggcaatg
     2161 ctgcattttg ttctgccgga gtttgccgct atctataaga ccttcaacac cccactaccg
     2221 gcactaacgc aggggatcat gacgctggca gactttagtg gcgaatggag ctggctgctg
     2281 gtgttgttcg gctttctgct ggcgatagcc aataagttgc tgatgcgccg accgacctgg
     2341 cttatagtgc ggcagaaatt gctgttacgc atcccgatta tgggttcact gatgcgggga
     2401 caaaaactca cgcagatctt tacgattctg gcgctgacac aaagtgcagg cattactttt
     2461 ttgcagggcg tagagagcgt cagagaaaca atgcgctgcc cgtactgggt gcaacttctg
     2521 acacaaatcc agcacgatat cagtaacggt catcccatct ggctggcgct aaaaaatacc
     2581 ggggagttta gcccgctctg tttgcaatta gtgagaacag gagaggcatc cggctcgctg
     2641 gacctcatgt tagacaacct cgcccatcat catcgggata acacaatggc gctggcggat
     2701 aacctcgcag ccttactgga accggcgttg ctgatcataa cgggaggaat tatcggtacg
     2761 ctggtggtgg cgatgtatct gccaattttc catttaggcg atgcgatgag tgggatggga
     2821 taacgctggc gtgttgctcc acaccagcgt tggaagatta caggttgttg aagatgcggt
     2881 tttcttgttc ctgcacacgg ataaatgtgg tgcgcttggt cagttctttc aggcgtgaag
     2941 ccccaacgta tgtacaagct gaacgcaggc cgcccaaaat atcacgcgcg gtattttcaa
     3001 ccgggcctcg cagcggcagc ttaacggttt taccttctgc tgcgcgatat tccgcaacgc
     3061 cgccaacgtg acgtttcatc gcagactcgg agctcatgcc gtagaacagc ataaatttct
     3121 cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg gcccgccagc atgccgccaa
     3181 gcatgacgaa atcggcaccg ccgccaaagg cttttgcgac atcgcccggc gtggtgcagc
     3241 caccgtcgct gacgatcatt ccgcccagac cgtgcgccgc atcggcacat tcgattaccg
     3301 cagaaagctg cggataaccg acgcctgttt tgacgcgagt tgtacaaaca gaacctgggc
     3361 caatgccaac tttaacgata tcagcaccgg agaggataag ctcctcacac atttcaccag
     3421 tcactacgtt gccagcacaa atggttttgg tcggccacgc ttcacgcgct ttcgcaacga
     3481 actgcacgaa gtgttcggaa taaccattcg ccacatcaat acaaacaaag tttaatgccg
     3541 ggttcaggtc gagaatctgt ttagtttttt cgaaatccgc atcagacgta ccggtagaaa
     3601 ccatcacatg tttcagcaca tcagcggaag aattgttgat aaacgcttgc cactcttcga
     3661 cagaatagtg tttatgcaca gcagtcaaaa tatcaaaaga agccagcgca gaggccatag
     3721 aaaatgtacc tacggtgtcc atatttgcgg cgataatcgg cacgccggac cagctctgat
     3781 ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc ggaacggctt ttaagagtgg
     3841 agcgtttagg gcggatgaga acgtctttaa aacctaactt cagatcttct tcaatacgca
     3901 tgtgcggatt cctggggtta atggctataa gggtaagagc gcaactccag tgacgctatc
     3961 atacgcacta atcaatgcgg cgcaagactg cgaaattgtc tttttttacg ctacaatccc
     4021 ataaatttac ctggtaaata gtgagcgaac gtttcatcaa tttcgctttc gtgcactcat
     4081 ttttaacgtt ttgataatca tgatgaattc ccgggaatat aaattatgag gtatatagtt
     4141 gccttaacgg gaggcattgg cagtggcaag agtaccgttg ccaatgcgtt tgctgatctc
     4201 ggaattaacg tcattgatgc cgatattatt gcgcgtcagg tggttgaacc aggtgcacct
     4261 gcgctacatg ccattgctga tcactttggc gctaacatga ttgctgctga tggaacattg
     4321 cagcgccggg ccttgcgcga gcggatcttc gccaacccgg aagagaaaaa ctggcttaac
     4381 gccctgctgc atccgctgat tcagcaagag acgcaacacc agatccagca agctacttcc
     4441 ccctatgtac tgtgggttgt gccattgctg gtagaaaact cactgtataa aaaagcgaat
     4501 cgagtgctgg tggtggatgt cagcccagaa acgcaactta agcgcaccat gcagcgcgat
     4561 gatgtaactc gcgagcatgt cgaacaaatc cttgctgctc aggcaacgcg cgaagcccgc
     4621 cttgccgtgg cagatgacgt cattgataat aacggcgcac cggatgctat cgcatcggat
     4681 gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc agtttgtctc acaggaaaaa
     4741 ccgtaatgca gacccaggtc ctttttgaac atccactaaa tgaaaaaatg cgtacatggc
     4801 tgcgcattga gtttttgatt cagcaactca ccgttaattt acccatcgtt gaccacgctg
     4861 gcgcgctgca tttcttccgt aatgtcagtg aattactgga tgttttcgag cgcggcgaag
     4921 tccgcactga gctgttgaaa gaacttgacc ggcagcaacg taaactccag acttggattg
     4981 gcgtgcctgg cgtggaccag agccgtattg aagcattaat tcagcagtta aaagcggcgg
     5041 ggagcgtatt aatttccgcg ccgcgtatcg ggcaatttct gcgtgaagat cgtttgattg
     5101 ctctggtgcg tcagcgactg agcatcccag gcggctgttg cagctttgat ttacctacat
     5161 tgcacatttg gctgcatcta ccccaggcgc agcgcgacag ccaggtagaa acctggattg
     5221 ccagcctgaa cccgctcacc caggcactta ccatggtgct ggatttaatt cgccagtcgg
     5281 cccccttccg taaacaaacc agcctgaatg gtttttatca ggataacggt ggcgatgccg
     5341 acttgctgcg cctgaatctg tcgctcgatt cacagcttta tccgcaaatt tccggacata
     5401 agagccgttt tgccattcgt tttatgccgc tggacagtga aaacggacag gtaccggaac
     5461 gtctagattt cgaactggcc tgttgctaag gagtgaaaat gtcagaaact attacggtga
     5521 attgcccaac ctgcgggaaa acggtggtat ggggtgaaat cagcccgttt cggccatttt
     5581 gctccaaacg ttgtcagctg atcgacctcg gagaatgggc tgctgaagaa aaacgaatcc
     5641 ccagcagtgg cgatctttcc gaaagcgatg actggagcga agagccaaag cagtgacatt
     5701 ggtctgatgc cgcacatagg cctgataaga cgcggacagc gtcgcatcag gcatcttgtg
     5761 caaatgtcgg atgcggcgtg aacgccttat ccgacctaca gactacagac gcttaagctt
     5821 cgcaattacc ggttcattgg ctggcggaaa atcatcggca ttaagaccga ccagcgacat
     5881 ccactcaccg ggttgccctt ctttacccca cggcacccct tcccaacttt cgaccagcca
     5941 gaaccacaga gttatatgcc tgtccgggaa ttcatatgcc agtttttcaa atagcgaaaa
     6001 atgttgtggg gtaatcccga cctcttcctg aagttcacgc accaccgcct gttccggcgt
     6061 ttcgcccatt tcaattttac cgccgggaaa ctccagttta ttcgccatgt gcgcatctgc
     6121 tgcgcgacgc gttataaaga tttcattgtt ctcgttgcga ataataccta ccgcaatttg
     6181 cagctttttc attgttttaa acctataaaa aaggcgcaga atcctgcgcc ttttgcttca
     6241 acagttagct tttattgcag gcggccatga cactgcttgt attttttacc agaaccgcac
     6301 gggcaaggat cgttacgtcc tactttgcgc tcaccggttt gcgccgccag tgcagctgcg
     6361 gctgcagagt cgtcatcctg atggctaagc tgctgcattt gcgctaaacg ctcggcttcc
     6421 atacgacgct gttgttccag ctcctcaacc tcttcaggca tacgtacctg aactttgctc
     6481 agcgtactga taacttcata tttcaacgac tccagcatcg ctgcaaacat ggagaacgat
     6541 tcacgtttgt attcctgctt cggatctttc tgtgcatagc cacgcaggtg gataccctga
     6601 cgcagatagt ccatcgctgc caggtgctct ttccacaggg agtcaagcgt ttgcagcatg
     6661 acgcctttct cgaagtgacg catcatctca gcaccaacca cttcttcttt acgctgatac
     6721 acttcgatgg actgcgccag aatgcgctca cgcagcgtct cttcatgcag ttctggttct
     6781 ttatccagcc actcggcaat tggtaaatcg aggtcgaaat cgttcttcag acgttcctgc
     6841 agccccggaa tatcccacat ttcttccagc gactgtggcg gaatgtaggc atcaatggtg
     6901 gctttgaaca catcttcacg aatgctgttg atggtttcgc tcacatcgct gacatccagc
     6961 agttcgttac gctgggagta aatggcgcga cgctgatcgt tagccacgtc atcatattcc
     7021 agcagttgct tacgaatgtc gaagttacga ctttcaactt tacgctgggc gttggcaatc
     7081 gctttagtca cccacgggtg ttcaatggct tcgcctggct tcatacccag tttacgcatc
     7141 atgccggata ctcggtcgga agcaaaaata cgcatcagcg catcttccat cgacaggtag
     7201 aaacgggaag aaccagcatc cccctgacga ccagaacgac cgcgcaactg gttatcgata
     7261 cgacgggatt cgtgacgctc ggtaccgatg atatgcaggc cacctgcttc cagtaccgca
     7321 tcgtgacgta cctgccagtc ggctttaatt ttttcaattt gctctgcggt cggattttcc
     7381 agcgcggcaa cttctgcctg ccagctacca ccgagcacaa tatctgtacc acgacccgcc
     7441 atattggtcg cgatagtcac cgcagccgga taacctgctt gagcaacaat cgccgcttcg
     7501 ttggcgtgga atttggcgtt caggacgttg tgcttaatac cggctttggt cagttcgttt
     7561 gacaccagct ccgatttttc gatggagata gtacccacca gcaccggctg gcctttcgca
     7621 gtacgttctt tgatatcttc aatgatcgcc tgaatttttt ccgcttcagt catgtagacc
     7681 aggtccggca gatctttacg aatcattgga cggttggtcg gaacaacgac ggtatccagc
     7741 ttgtagatgg agctaaattc gaaagcttcg gtatcagcag taccggtcat ccccgccagt
     7801 ttttcataca gacggaagta gttctggaag gtgatcgaag ccagcgtttg gttttcgttc
     7861 tggatctgca caccttcttt cgcttccaca gcctggtgca gaccatcgga ccagcgacgg
     7921 ccctgcatgg tacgaccggt gtgttcgtca acgatgataa cttcaccatc tttaacgatg
     7981 tagtcgacgt cgcgggtaaa cagcgcatga gcgcgcagcg ccgccgttac gtggtgcatc
     8041 agcatgatgt tggccggaga gtacagagac tccccttcat ccatgatgcc ctctttcacc
     8101 agcagttctt caatcagcac cagaccacgt tcggtcaggt tcacctggcg agatttttcg
     8161 tccaccgaga agtggccttc gccctggaag gtttcggagt cttctttttc ctgacggatc
     8221 aggtgcggaa taattttatt cacgcgttta tacatttccg agctgtcttc tgccgggccg
     8281 gaaatgatca gcggtgtacg cgcttcatcg atcaggatgg agtccacttc gtccaccagc
     8341 gcatagtgca gtttacgctg tacacgttct tcagggctga acgccatatt gtcgcgcagg
     8401 tagtcaaagc cgtattcgtt gttcgtaccg taagtgatgt cagctgcgta agcttcgcgc
     8461 tttgccggtg ctggcatgcc cggcaggttg ataccgacag tcaggccaag gaattcaaac
     8521 agcggacggt tgttttcggc gtcacgttgc gccaggtagt cgttgacggt aactacgtgc
     8581 acgcctttac cggttagtgc gttcaggtaa gcaggcagcg ttgcggtcag ggttttacct
     8641 tcaccggtac gcatttcggc gatgcagcgt tcgttaagaa ccataccgcc gagtaactga
     8701 acgtcgaagt gacgcatacc aaagacgcgc ttacttgcct cacgtaccac ggcgaaagct
     8761 tccgggatca gattttccag cacttcgcct ttttccagac gtgcacgaaa ctctgcggtt
     8821 ttccctttca gttcttcgtc ggagagtttt tccatctccg gttccatggc attgatgatg
     8881 ttgaccactt tgcgcatccg gcgcagggtg cgatcgttac gactaccgaa aactttagtt
     8941 aacaatttga ttagcataat aaaatctcaa acgccccgcg ttgcggagtt aataaaatga
     9001 agtaaaggtt tattgttgtt aggtgaggcg ttgagggcca gcacggatgc cttgcgcctg
     9061 gcttatccag acgggcgtgc tgaattttgc ttgtggggta aaatgcgcat aatcaatgcg
     9121 ataacccttt tcagacggcg tgccttcctg ggtcagcagc gcgctgagcg tatccagtaa
     9181 tgcaagatgt tgcgcctgaa gaggcaaaga ttcttcagca acgggcagtg tttgcggtgc
     9241 cattgcgaaa gaaagatgac ggattaccgt gcgaatggca tattgatgcc agtaatcaac
     9301 ggaatagttc gaattcgggc ggcgtgtgtt cgcttccagc aaggccaatt gaccaaagtt
     9361 aactttggct gaaggctcgt ggttgcgggt tgtcgctttt gcgggcacgt ttggttcggc
     9421 ggcgttgctg agcgcaggca aacctaaact cgccgcgacc atccctaata agagatgcgg
     9481 ccagaagtag cgtttaccaa actgtcgcca gcgcgtcagt attccactca cgttattgcc
     9541 atcccgatcg aattcaaacg tatttagcgc acaaatctta tcattaaagc ctgaagtcca
     9601 cagcatgaat gaagataagt tgtgcgtaaa agtgcttaaa aaaacagcgt tcgcaccgtt
     9661 ttttatagct cttataaaat tcaaaaaaaa acgactggtt cacctggccg gagagagtgc
     9721 cagatttgcc agtcgaattt tatacgacag tataaatgtc gttatgccag tacagctgaa
     9781 ggcgctttga aggccaacgg cagttctgcg tcgtcctgga aggtcacata ttcccaagct
     9841 tcctgtttcg ccaggacagc ctgcagcagt ttgttattca gtgcatgacc ggatttataa
     9901 gcgataaatg caccaataat attgtgacca cacatgaaca agtcaccgat cgcatcgagc
     9961 attttgtgac gcacaaattc gtcttcaaaa cgcaggccgt cttcgttcag tacgcgataa
    10021 tcgtcaacaa cgatggcaca atcgaagctg ccgcccaggc acaaaccacg ggactgcaga
    10081 tattcgatat cacgcatgaa accgaacgta cgcgcacggc tgatctggcg cataaacgca
    10141 tcagcggaga agttcatcgc atagcgctgg ttgctggaat caatagccgg atggttaaaa
    10201 tcgatggtga aatccagcga aaaaccattg tacggcttaa attcagccca cttatcgcca
    10261 tcttcgacac gaacagtctc tttgatgcga acaaattttt tggcgcagtt caactcgtcg
    10321 ataccggcgt caagcagcag gtatacaaac ggagcggcgc tgccgtccat gatcgggatt
    10381 tccggcgcgt taacttcgat aacaatgtta tcgatgccca ggcccgcgag agcagcattg
    10441 aggtgctcta cggttgaaat ccgtacatca tgctcgttga ccagacacgt acagagcatg
    10501 gtatcacgca cagatttggc atcggccggg aaatctaccg gtggattcaa gtcggtgcga
    10561 cgatagatga ccccggtgtt ggccggcgca gggcgtaacg tcagagtgac tttcttgccg
    10621 gtatgtaaac cgacacccgt cgcctgaacg atacgtttaa gtgtcctttg tttgatcatc
    10681 gtattatctc gccaaattac ctatccaacc gaagtgtact atacattcgg cgggccagtt
    10741 tagcacaaag agcctcgaaa ccaaaattcc agtcaattct taatcagctt gcttacgcag
    10801 gaatgctggg atatccagat aatccggctc tttcgcagtt tgcggcgcat tgtcattcac
    10861 gactttagca accggcttct gctcctgggt cagcggagcc atcccatgct gctggtagcg
    10921 atccatcact ggctgctgaa cctgcttatt ggtcaccaga gtgatttcag gacgtttgtc
    10981 catgccgata cctgtcgcaa caacggttac gcgcagctcg tcattcatat ccgggtcaag
    11041 agaagtaccg ataaccacag tcgcgttgtc ggaagcaaat gcacggatgg tgttacctac
    11101 cgtttcgaac tcatccagac gcaggtcgaa gcccgccgtg atgttaacca gcacgccgcg
    11161 cgcgccagac aggtcgatat cttccagcag cggagaagag atagccattt cagcagcttc
    11221 ttccgcacgg tcttcaccgc tcgccacgcc agaacccatc attgcgtagc ccatctcaga
    11281 cattacggtg cgtacgtctg caaagtccac gttcatcaaa cccggacgag taatcagttc
    11341 agcgatacct tgcacagcgc ctttcagtac atcgttcgct gcgccaaacg catccagcag
    11401 ggagataccg cggcccagaa ctttcagcag tttgtcgttc gggatagtga tcagagagtc
    11461 cacatgcttg gacagttcag tgatcccctg ctccgcgaat gccatacgct tcttgccttc
    11521 aaagttgaaa ggcttagtga cgacagcaac ggtcaggata cccaaatctt ttgccacttc
    11581 agcgacgact ggtgctgcac ctgtaccggt accaccaccc atacccgcag caataaagac
    11641 catgtctgca ccttccagcg ccgcacgcaa tgcatcgcga tcctcatcag ccgcattgcg
    11701 gccaacttct ggattagcgc cagcgcccag tcctttggtg ataccgctac cgatttgaat
    11761 cgtctgtcca accgctgttt tacgcagcgc ttgtgcatcg gtatttaccg cgaagaattc
    11821 aacaccttca atgcgctcgc gcaccatgtg ttcaacagca ttaccgccgc cgccgccgac
    11881 gccgatgact ttaatcaccg cgtcattggt aagttccatt ggttcaaaca tagtttctct
    11941 ccgatttgtg cctgtcgcct gaggccgtaa tcatcgtcgg cctcataaaa attaaaactc
    12001 ttttcgcagc caactattga gtcgcttgat ccacgagcca actgatgctg taacacgttt
    12061 ttctacttca gcttcaccgt taagatgtga ctctttccca tagtgaagca atcccaccgc
    12121 cgtcgaataa tacggctcct gagcataatc cgttaaaccg gtaatgttca gcggcgcgcc
    12181 gatacgcact tgcgtatgaa acacgcgctg agcacaggct gcaagacctt cgatctgcgc
    12241 tgcgccaccg gttaatacaa tgcctgccgc caggtgatgt ttaacccctt gttggcgaag
    12301 cttttcctgc aactgcaata tctcttcgtt gaccaggttg agcagctcgg tatagcgcgg
    12361 ctcgatcacc tctgccaggg tctgacgttg cagactccgt ggcggacgac cgcctacgct
    12421 cggcacttcc acgctctcat cttttccaac gatggaaccc agcgcacaac cgtggcgaac
    12481 tttaatcgct tcggcgtcgc ttggcggcgt gccaaaggcg taagcgatat cactggtcac
    12541 gacattgcca gcataaggaa ttaccttagt gtggcgcaat gccccaccgg tataaacggc
    12601 gatatccatt gtaccacctc cgatatcgac gacgcagaca cccagttcac gttcatcttc
    12661 cgtcaatacc gaataacttg atgccagtcc ggcaaatatc agttggtcaa ctttcagccc
    12721 acaacgttca accgctttga cgatgttttt cgccatatcg ttgtgacatg tgatcaggtg
    12781 cacttttgcc tgcatccgca cgcccgaaag tcctaccgga ttcttgatcc cttcctgata
    12841 gtcaatcgca tactcttgcg ggatcacatg cagcacacga tgttcatcgc gcacacgcac
    12901 cgatttcgcg gtatggacga cgttttccac atcttcttgc gtcacttctt cttcagaaat
    12961 aggcaccata ccaatttcat tctggcagct gatgtgctta ccagaaagcg ccagatatac
    13021 cgaagagatc tgacaatctg ccatcaattc tgcctggtca atggcgcgtt gtacgcactt
    13081 gaccacggat tcgaggtcgt tcaccccgcc tttatccata ccacgtgacg ggcagctgcc
    13141 cacgccaatg atattgacca taccgtcggg cagaacttcc cctactaaag cggcaacctt
    13201 cgcggtacca atctccagtc ctactaccag ttttctgtcc gtcgccttga tcattgttgt
    13261 tctgcctgtg cctgattttg ttgctgagta gattcctctg gcggcaaggg cgcccagcct
    13321 actgccgctc cagagtcata acgcaaatca acgtagctaa tccgtttgcc atcggtttgc
    13381 gccagctgct gtaaaaccgg ataaagttct acaaagcgag ccaaacgttt catcgtatcg
    13441 ccccggccaa gattgagctt aatatcgtta ttcagcgtca actgccagga acgccgcgcg
    13501 gtcatcgccg tttccttcag agtaaatctg tcctttgcca gcatctgccc catttcgcga
    13561 tagccctgca acacttcatt ggcgctgcct tccgggccat acagcattgg aagcacctgc
    13621 ttgctggtgc gatctggcgg cacgctgaag gtgtttcctt ccgcgtctac catatgttga
    13681 tcattccacc gcgcaatcgg cacatattca accagatgaa tcttcaattc atcaggccac
    13741 tgctttctga cgctcacctg cttaatccac ggcaggcgtt gttctatttg cgtctggatg
    13801 atgttgacat cctgggtcat aaaggtaccc ggctcaccca atgccaggat cgactgccgg
    13861 atatcgtcat tacgcgtgta atggcgttca ccggtcaaca ccagctttga gagcggcagg
    13921 cgttgcgcat cttccatcca gcccaacacg acccagccgc tcaccaacac tgtcgttaaa
    13981 acggtcagca ggaaaaggat ccccgccaga cgcgttccat tattgcggcg agaagaaacc
    14041 tcttcttcgc tgtttcgcgt gttcagagca gcctgcgaca tattagtccg ccagttccag
    14101 aattcgtact accaactgcg agaagctcat acctgcctga cgtgccgcca tcggcaccag
    14161 gctgtggctg gtcatacccg gtgaggtatt ggcttccagc agataaaact gtccatcgct
    14221 gtccagcata acgtcaatac gtccccatcc tttgcaacct aacgtcgtcc atgctttcag
    14281 cactaatgcc tgcaaattgg cctcttgtga cgcatccaga cctgcggggc agaaatactg
    14341 tgtctcatca gagagatact tcgcctcata atcatagaag gttccggacg gttgaatacg
    14401 tattgacggt aaaatttctt caccgagtat cgcaaccgtg aactccggcc cacttagcca
    14461 tttttcaatc aatacttctt catcgtgctg aaatgccaat cttaatgcat cttgtagagc
    14521 attttctgct actacttttg acattcccac actggaacct tcgcggctcg gcttaacgat
    14581 aaccggcaaa cccagagcag aaatttctgc taactgctta tcgctcaggc ctttttcaaa
    14641 ctctgcgcgg gttaacgcta cccacggcgc gaccggtaaa ccggcacctt gccacagcag
    14701 tttgctgcgt agtttatcca ttgaaagcgc agatgccatc actccgctgc cggtataagg
    14761 caagcccatc agctcgagca tgccctgcag cgtaccatct tcaccgccgc gaccgtgtag
    14821 cgcgataaac actttctgaa aacccatcga cttcagttgc gtcacgtcga cttctttcgg
    14881 gtcgacagga tacgcatcaa taccaccttc acgcagcccg gctaacaccg ctgcgccaga
    14941 attcagagaa acttcccgct cagcggaagt cccgcccaac aggaccgcga ttttatcagt
    15001 catgttgttc ttcctccgga gtttgcggct tcagtttgat ttcagctaaa gaacgggcaa
    15061 tttttccgat attaccagcc ccctgaacga gaatcaggtc gttaccggtt aataccggtg
    15121 ccagcatctc ggctacctgc gcagggtccg gcaccagaat gggatcaatt ttcccacgtc
    15181 cacgaattgt gcgacacagc gaacggctgt ccgctcccgg aattggcgct tcgccagccg
    15241 gatacacttc cagcatcaac agggtatcaa cctgcgtcag cacattggcg aaatcatcat
    15301 acaagtcgcg cgtacgggta aaacggtgcg gctgaaacag cattaccagg tttttatccg
    15361 gccagcctgc gcgcgccgct ttaatggtgg cgtcaacttc cgtcgggtgg tggccgtagt
    15421 catcgaccag cattgccgta ccgcttttac cattcactgg ctccagcggg aattcaccga
    15481 ggaaatcaaa acggcgacca gtccccagga agctctccag cgcacgcaaa atagcctcgt
    15541 cgtcaattcc ctcttccgta gcaaccgcaa ccgcagctgc ggcgttcagc gcgttatgac
    15601 ggcctggcgc attcagggtg acgcgcatcg gctctttgtc ctggcgcagc agcgtaaagt
    15661 gcccctgcgg gccaatctgc tgataatctt ctacacgcac gtcggcatct tcgctgaagc
    15721 cgtaagtcgt ggtctgacgt cccacacgcg gtaacaattc gcggatcacc ggatcatcaa
    15781 cacacatcac cgcacgaccg taaaacggca ggttgtgcag aaaattaata aaagtctgtt
    15841 ttaaattctc aaagtcgccc tggtaggtat ccatgtggtc ggcttcgata ttggtgacaa
    15901 tcgccaccat cggttgcaga tgcaggaacg atgcatcact ctcatctgct tcggcaatca
    15961 ggtaccgacc atgccccaaa cgcgcatgaa cccccgccgc ttttaccagc ccgccgttaa
    16021 cgaaggttgg gtcgagcccc gcttctgcgt agatgctgga aaccatcgcg gtggttgtcg
    16081 ttttgccgtg cgttcctgca atggcgatgc catgacgaaa acgcattaac tcagccagca
    16141 tttcggcacg acggatcacc ggaatacgcg cttcatgagc tgcgacaatt tccgggttat
    16201 cggcagaaat ggcgctggaa acaacgacca cgctggcatc acgtacgttt tccgggcgat
    16261 ggttgaaata aatcgtcgct cccagattca ttaactgctg cgtgaccgga tttggcgcta
    16321 aatcggaacc actgatctga taaccttcat tggccagaac ttcggcaata ccgcccatac
    16381 cggcaccacc gatgccgaca aaatgtatgt gccgaacgcg acgcatttcg ggcacgatgg
    16441 aacgcagttt tgccaattgt tgtgtattca ttctttacgc cattaacttc ttaaattcat
    16501 acgatgcaaa aggcatcgct acaattacgc ccgggcagcc cggctcactt catttgccac
    16561 tcgctcggtg gcatccggaa tggatgcagc gcgggcgcgt tctgccatgg ttaataaggt
    16621 ttctcgcgac cacccggcca gggtgttggc gacagcatcc acgctaagct gtggctgctc
    16681 gataattttg gctgcgcccg ctttttccag cggtagcgca ttccagtatt gctggcggtc
    16741 tttatgttga aacggcacaa acaacgccgg tagtcctgcc gcggcgattt cactcaccgt
    16801 taaagcaccg gagcggcaaa cgacgacatc cgcccacgca tacgccgccg ccatatcatc
    16861 aataaattcc gtcactttat gctgcggttg ccccgcttcg gcatacgcct gttcaacgga
    16921 ttgttgcgaa cctttaccgc tctgatgcca gatagtgact gaatcaccca gtttcgcagc
    16981 aacctgcggc attgtctggt taagaatgcg tgcgccctgg gaaccaccca ctaccagcac
    17041 acgaaccgga ccttcacgtc cagccaaacg ttgctgcggc aacggcagcg ccagcacatc
    17101 ggtacgcacc gggttaccca ccacttccgc attagggaaa gcgcctggaa acgcctgcat
    17161 cactttggtg gcaatcttcg ccagccattt attggttaag cccgcaatac cgttttgttc
    17221 atgaagtaca accggaatgc ctaacgacca cgcggccaga ccacctggac ctgatacata
    17281 gccgcccata ccgagtacca cgtcaggttt gtacgctttc ataatcgccc gcgcctgacg
    17341 ccaggcgttg aagatacgca gcggcgcagc tatcagtgct tttatacctt ttccacgcag
    17401 accagagata cgaatgaaat caatttcgat gccatgtttt ggcactaagt ccgcttccat
    17461 acggtcggca gtccccagcc agcgaacttg ccaaccctga gccattagat ggtgcgcaac
    17521 cgccagtccc gggaatacat gtccaccggt tccgcctgcc atcaccatta atcgctttcc
    17581 ttgaccactc atcgtgaacc tcgtacaaac gcctgcgctt tctccagacg cgtttcataa
    17641 tcaatacgca acagcatcat gatggctgtc gacataatca gtaagctcga accaccgtaa
    17701 ctgatcagcg gcaatgtcag acctttggtc ggtaacatcc ccgccgccgc gcctacgtta
    17761 accagcgcct ggaagctaaa ccagatgcca atagaacagg cgagaaaacc ggaaaaacgg
    17821 tggtcaattt ctaacgcttt gcggccaatc gacatcgcgc gaaaagcgac gaagaatacc
    17881 attaaaagtg ccagcaccac accgacatac cccagttctt cgccgataat ggcgaaaata
    17941 aagtcagtgt gcgcttccgg cagatactcc agtttttgta ccgagttacc taaaccttgt
    18001 ccccaaagtt cgccgcgacc aaacgccatc agcgattgcg ttaactgata gccgctgcca
    18061 aagggatctt cccacgggtt ccagaatgcg gtaacacggc ggatacggta cggttcggcg
    18121 agtatcagca acacaaccgc tgaaatgccc ataccgataa tggcaatgaa ctgccacaat
    18181 ttcgctcccg ccaggaacaa catcgccagc gtagtcacaa acaccaccac caccgtacca
    18241 aggtctggct gtgccagcag taacactgcc aacaccagaa tcacgcccat cggtttcagg
    18301 aagccgcgca ggttattacg tacttcgtcg cctttacgca ccagatagtt ggcgatataa
    18361 caaaacagcg acagttttgt cagctccgca ggctggatac gcagcaaacc gagatcgatc
    18421 caacgcgatg cccctttaac cgagctcccc actaccagca cgatcatcag caggatgata
    18481 gatccgagca gcatcgtggc actgtagcgt tgccagaact ccatcggcag acgcagcgta
    18541 atgatcgcca gaatgaatgc caggatcaga tagacgccat cacgcttcgc gaagaagaac
    18601 ggatcgttgg ttaagcgttg ccctatgggc attgacgccg aggtcaccat gataaagcca
    18661 atcgccgcaa ggccgaaggt cagccacagt aaggtgcgat cgtacatgat caggctgtcg
    18721 gtatcttttt cccgcgagcc catcacccag ccttttagcg ccgtggagat ccagaccagg
    18781 atactgaatc ctggcaggcg cggcattttc aggcgaggga gagataaacg catcaaccta
    18841 actccttcgc cagacgggca aactcattgc ctcgttgttc aaagttcttg aactgatcaa
    18901 ggctggcaca ggccggggag agcagaacca tatcgcccgg ctgaacacgc ggagccagca
    18961 agcgcatcgc ctgttccata gtttcggttt gttctgccac ttccgggcgt agcgccgcca
    19021 gctgcgcacc gtcacgacca aaacagtaca aacgcacgtt gtcgccattc agatagcgcg
    19081 ccagtggact gaagtccgcc gatttaccat cgccgcccag caacaaatgc agcgtgccgt
    19141 ctacctgcaa gccattcaac gccgcttccg tactgccgac gttagtggct ttcgaatcgt
    19201 taatccagcg tacgccattg tgctccagca ccacttcaaa gcgatgcggc agaccggtga
    19261 aggtggttaa cgctttcagg ctgctggcgc gcggcaaacc agctgcgtct gccagtgcca
    19321 gtgctgccag cgcattggtg tagttatgct gcccggaaag tttcatctct ttcacgttca
    19381 gcactttctc gcctttcact cgcagccagg tttctccctg ctgatgattc aggtgatagt
    19441 cacccatgtt gacgccaaag ctgacgcagc gttcatcagc accgcgaatc ggcattgtta
    19501 aggcatcatc agcattgacg acacaaactt tcgcattttc gtaaatacgc aattttgccg
    19561 cacggtattg ctgcaaacca aacggatagc ggtccatatg atcttcagtc acgttcagaa
    19621 tggtcgccgc caccgcctgt aagctggaag tggtttccag ctggaagctc gacagttcca
    19681 gcacgtacag ttcgcactca gcatcaagca gcatcaacgc tggcaggcca atattgccac
    19741 ccacaccaac gttaaccccc gccgctttcg ccatttcacc cactagcgtg gtgaccgtgc
    19801 ttttgccgtt agaaccggta atcgccacaa tcggtgcttg tgcttcgcgg cagaacagct
    19861 cgatatcgcc aacgatttcg attccggcat cagcggcagc gcttaaggat ggatgcgcca
    19921 gtgcaatacc gggactggcg acaatcagat ctgccgccat cagccattca tcattcagac
    19981 tgcccgtgtg gcgttctacg gcttcgggta atttatccag gccaggcggt gtcatacgcg
    20041 tatccataac gcgcggcgtc acaccgcgag cgaggaaaaa gtccacgcag gaaagcccgg
    20101 tgaggcccag gccgataatg acgacatttt taccctgata atcagccatg attaacgtac
    20161 cttcagcgtt gccagaccaa tcagaaccag catcagcgaa ataatccaga aacgcacaat
    20221 gacgcgcggt tccggccagc ctttcagttc atagtggtga tgaatcggtg ccatgcggaa
    20281 aatacgttgt ccgcgcagtt taaaggagcc gacctgcagg atgacagaaa gcgtttctac
    20341 cacgaacacg ccccccataa tcaccagcag gaattcctga cgtagcagta cggcgataat
    20401 gcctaacgca ccacctaacg ccagcgaacc tacatcgccc ataaagacct gcgccggata
    20461 ggtgttaaac cacaggaagc ccagtcctgc cccgactatc gcggtacaga caataaccag
    20521 ttccccggcg tgtcgcagat acggtatgtg taaatagctg gcaaagttca tgttgccggt
    20581 cgcccacgcc accagcgcaa aaccaccggc gacaaatacg gtcggcataa ttgccaggcc
    20641 gtcgagacca tcggtcaggt ttaccgcgtt gccagtaccc acaatgacga agtaagccag
    20701 cagaatgtag aacagcccca actgcggcat cacatcttta aagaatggga ccaccagctg
    20761 cgttgcgggc gtgtctttgc cggcaaggta cagggcgaag gcgacaccca gcgcaatgac
    20821 cgacatccag aaatacttcc aacgagcgat caaccctttg gtgtctttac gcaccacttt
    20881 gcgataatca tcaacaaagc caataacacc gtaacctacc agcaccacca acacgcacca
    20941 gacgtacgga ttggacgggt aagcccacag cagtacggag atcacaatcg ccgtcaggat
    21001 cataatcccg cccatggtcg gcgtaccgcg cttgctgaag tgtgattcag gaccgtcgtt
    21061 acgcaccacc tgaccaaagg aaagtttttg caaatgagca atcatacgcg ggcccatcca
    21121 caatgagatg aacagcgcgg tcagcaggct gacgatggcg cgaaacgtca gataggaaaa
    21181 gacgttaaag ccggaataat atttgaccaa atgttcggcc agccaaacta acatgtccca
    21241 ttctcctgta aagcgcgtac tacctcttcc atggcggcac tacgtgaacc cttaactaaa
    21301 atcgtaatta cctgttgctc agcaatcagt gatttaagac gcgtaattaa cgcagtttta
    21361 tcagcaaaat gttcgccaac gccgctggcg gtgctgatag catggctttg tttacccacg
    21421 cttaacacgc ggtcaatacc agcagctttt gccgcctcgc ccacctgtac atggcaggct
    21481 tcgctttcag cgcccagttc cgccatatcg cccaccacca gcacgcggta gcccggcatt
    21541 tcagccagta cctgaacagc ggcggtcatt gaaccgacat tggcgttgta ggagtcgtcg
    21601 agcagcaact ggttttctgc cagttggatg gggaacagac ggcctggaac agctttcaga
    21661 tttgccagcc ccgctttgat agcatcaagc gttgcgccca cggacatgga gagcgcagcg
    21721 gctgccagcg cattcgcaat attgtgacgc cccggcaacg gcagcagaac atcgacgcta
    21781 ccggttgggg tttgtagggt aaattccgta ccgtgcgagg tcacatggat attggtggcg
    21841 gtgaaatcgc tgttggcggc attgggtgag aaacgccaca ctttgcgtga gccaattacg
    21901 ctctgccagt tcagccagtc gttgttgtcg gcgttcataa tggcgatacc gttttccggc
    21961 aggccgctaa agatttcacc tttcgctttc gcgacacccg caagcgagcc aaaaccttcc
    22021 agatgcgccg ctgccaggtt gttgaccagc gcagcttccg ggcgagtcag actcacagtc
    22081 caggctattt cgccctgatg gttcgcgcca agttcaataa ctgcgtaatc gtattccggc
    22141 gttaagcgca acaacgtcat cggtacaccg atgtcgttgt tgagattgcc tgccgtataa
    22201 agcgtgttgc cgcactggct taaaatcgcc gccgtcatct ctttaacgga ggttttgccg
    22261 gaggaacctg tcagagcaac cacgcgcgcc ggaacttgct ggcgaaccca tgcagccagt
    22321 tcaccaaacg ccagacgcgt atccttgacg attaactgcg gcaggtcgat atccagcgga
    22381 cggctgacca gtagtgcgcc cgcgccgcca gctttcgcct ggtcggcaaa atcgtgggca
    22441 tcaaaacgtt cgcctttcag ggcaacaaac aggcagcccg gcgtcagttt tcgggtatca
    22501 gtggttacag catcaagggt gatatctgca ccttgcagtt caccgttgag aatgtcggta
    22561 agttggctaa gggttacgct aatcatgcaa tcacccccag cagacgcgcc accgtgacgc
    22621 gatcggagta gtccagacgc tgattgccaa caatctggta atcttcatgg cctttgcccg
    22681 cgaccagcac cacatcgttc tctttagcct gcataacggc gcaagtcacc gcttcagcac
    22741 ggccttccat cactttggca tgtccggcat ctaacattcc cgccagaata tcgttgatga
    22801 tggcacgcgg ttcttcggta cgcgggttat cgtccgtcac caccgccacg tcagcaaact
    22861 cttcggcaat tgcgcccatc agtggacgct tacctttatc gcgatcgcca ccacagccaa
    22921 agacacacca cagcttgccc gcacagtgca gacgcgccgc ctgtaaggct ttttccagtg
    22981 catccggcgt atgcgcgtaa tccaccacca ccgtcggttt gcctggcaca gtgaacactt
    23041 ccatacgtcc gcaaaccggt tgcagacgcg cggcggtttt caacagatca gccaatggat
    23101 agccgagtgc caacagtgtc gccagcgcga gcagcaggtt gctgacgtta aaagcgccta
    23161 tcagatggct ttcaatttcg ccatcgcccc aacttgagct aaagcgaatc gtcacaccgc
    23221 tgtcgtgata gttcacgtcg atcactttta accagcgtcc gtgacagttc ggattaatat
    23281 gatcttccat tgataccgca accgcgtccg gcagctttgc cagccaacgg cggcccactt
    23341 catcgtcggc gttaataatc gcctgaccgc aatgatgttc agaataaagc agccatttcg
    23401 cagcttcgta gtgttccata tcaccatgat aatcaaggtg atcgcggctt aagttggtaa
    23461 agaccgacgc cgcaaatttc aatgccgcca cacggtgctg taccagcccg tgggaggaaa
    23521 cttccattgc gcaaaacgtc gcgccctgct ccaccagccc cgccagctca tgctgaacat
    23581 cgactgccga accggtggta ttttctgtcg ggatcacttt ccccagcaga ccgttaccaa
    23641 cggtgcccat taccgcgctg gtttcgccaa gcagttggct ccactgcgcc agcagctggg
    23701 tagtcgtggt tttgccgttg gtgcccgtta cgcctacgag acgtaaattg tcagagggtt
    23761 catggtaaaa gcggcccgcc agtgcagata aacgctcgtt gagctggctg agatagatga
    23821 ccggtacgcc gtgcatttca cggatttcac cgtcggtcgc ctcatctttc gcctctgcaa
    23881 taatggcagc cacaccttgc gctatcgcct gcgggatata tcgacgcccg tccgcctgat
    23941 gacctactac agctacaaag agatcgcccg ccgcagccac acggctgtcg agtgtcatct
    24001 ctcgcagtgc tcgcgaaggt gcgtctggca cccacggagc aagaaggtcg cgcaaattac
    24061 gatctgccac ctgtcccctc gccttgatta atcacaaatt catttttatc gcccgttgtc
    24121 agcgcatccg gctcgatgtt catggtacgc aatacgccgc ccatgatggc accaaagacc
    24181 ggcgcggaaa cggcgccgcc gtagtattta cccgcctgcg gatcgttgat aacaacaacc
    24241 agcgcgaagc gcggctgact cgcaggcgca acgcctgcgg tataagcaat atatttattg
    24301 atgtagcgac cgtccggccc gacctttttc gcggtaccgg ttttaatggc gatacgatag
    24361 cctttaatcg ccgccttcac gccgccgccg cccggtagcg ccacgctttc catcatatgc
    24421 accacggtgc ggacaatgga ttccgggaag acacgttcac cgggaaccgg ggggtcaact
    24481 ttggtaatcg acagtgggcg ataaatgccg tagctgccga tagttgcgta gactcgcgct
    24541 aactgtaacg gtgttaccat tagcccgtag ccgaaagaga aggtggccct ctctatgtca
    24601 gaccaccgtt gtttttgagg atataagcca ctgcgttctc cgaccaaccc caaattggtc
    24661 gcttttccca gtccaaaacg tgagtaagta tctactaacg ctgaggacgg catcgctaac
    24721 gccagcttgg aaacaccgac gttactcgac ttctgtaata ccccggtcag ggttaattcg
    24781 ctgtagcgtg ccacgtcttt gatttcgtgg ccgttaattc gataaggaat ggtattgagt
    24841 accgagtttt cccgcaccac gccacgttgc aacgcggtca tcaccaccat cggtttaacc
    24901 gttgagcccg gttcaaacac gtcggtgatg gtacggttac gcatcgcctc tttcggcgtg
    24961 ccgctcagat tgttagggtt gtatgacggg ctgttagcca tcgccagcac ttcacctgtg
    25021 ttgacatcca ccagcacggc gctaccagat tcagccttgt taaaggccac cgcgttgttc
    25081 agttcgcgat aaaccagcgc ctgcaggcgt tcatcaatac tcagcgccag gttgtgcgct
    25141 gcctggctgt cagtagaaga aatatcttca attacgcgac catagcggtc tttacgcaca
    25201 atgcgctcac ccggctgccc ggtaagccat ttatcgaaac tcttctcaac gccctcaatc
    25261 ccttgactat cgacgttagt aaagccaatg aggtgagcag tcacttcgcc ggatggataa
    25321 taacggcgag attcttcacg cagatgaata cccggcagtt tcagtttttt gatgtagtcc
    25381 gccatgtcag ggtttacctg acgcgccaga taaataaagc gccctttcgg gttggcgtta
    25441 atgcgggcag aaagctgatc cagcggaata ttgagcgcgt tagccagcgc cttccagcgg
    25501 tcaccgacgc tgataccgcc agcgtcatgc acttctttcg ggtcagccca aatcgctttt
    25561 accggcacgc tcaccgctaa cgggcgacca gaacggtcag taatcatgcc gcgggaggtg
    25621 gaaacttgct gaacgcgaag agaacgcatg tcgccctctt tcaccagcat atccggggag
    25681 ataacttgta accacgctac gcgtccgagc agaaaagcca gcgccaggag aatacagccg
    25741 cataacaacg caaaacgcca actaataaag ttggcatgtt cttcctgacg ttttggtttc
    25801 tgcgttttcg ccgctgcttt catgcgtcgc gtttatcctt atttttgcac tacgatattt
    25861 tcttgtgacg gatcaacatg ctgcatttgc agcttttccg tggcgatcct ttccacccgg
    25921 ctatggtcgc cgagcgcatt ctcttcaagg atcaggttgc gccattcaat gtctaaagca
    25981 tctcgctcca gcaccagttg ttcgcgctga gcggtcagta aacgggtatg gtgcgccgtg
    26041 gttaccacgg tcaccgccgt caaaataatg caaatgaaca ggcagagtgg cagcttccca
    26101 aatcgcaaaa gatcgtcacc gataacacca ggcaatgcat ggcgctcgtg gcttcccatc
    26161 gatcctttaa ctttgcttag agcttctgtc actctgctga tcatgcgttc gtcctctctg
    26221 cgatacgcag aactgaacta cgggcacgag gattctcagc cacctcttct tcgcccggca
    26281 ttaacttgcc taatgctcgc agctgacggc cacccagttt tttgagctgc tcttcagtca
    26341 tcggtaaccc tgccggaact tgcggaccgc ggctgttttc acgcataaaa cgtttcacaa
    26401 tacggtcttc cagcgagtgg aagctgatga tcgaaagccg cccacccggg gccagcacgt
    26461 tgagcgagct ttttagcgcc tgctctatct cctccagttc actgtttacc caaatgcgca
    26521 ccgcctggaa ggtacgggtc gcgggatgtt taaacttatc tttcaccggc gttgcagcag
    26581 ccacgacttc cgccagttct ttggtgcggg tcatcggctg ttcgcggtta cgctcgacaa
    26641 tggcgcgggc aatgcgtttg gcaaaacgct cttcaccata ggttttcaat acccaggcga
    26701 tatcggcttc ttctgcggtt tgtagccatt cagcggctga ctgcccacgg gttgggtcca
    26761 tacgcatgtc cagcggacca tcgcgcataa aggaaaagcc acgttcagca tcatcaagtt
    26821 gcggtgaaga gacgccaaga tcgaggagaa tgccgtcgat cttgccgata agatcgcgct
    26881 cggcaacgta ttcgcccagc gcggagaaag gtccgtggat gatggagaag cgcggatcat
    26941 caatagtctt cgcaacggcg atagcctgcg ggtcgcgatc gatcgccagc aaacgcccct
    27001 cttcgccaag ctgcgagagg atcagacgtg agtgaccacc gcgaccaaaa gtcccatcaa
    27061 tgtagatgcc atcaggacgg atattgaggc cattaacggc ttcatccagc agcaccgtag
    27121 tatgtttata gttttccatc attttataga gacaagtcct gcagtcgctc cgataagtct
    27181 ccagtagcca actgctctgc gtcgatatct tccttgacct gttgatgcca ggttgtttca
    27241 tcccacagct caaacttgtt gaactgtcca accagcatca cttcttttgt cagcccggca
    27301 tgttgccgca gtactggcgc gattaacaat cgacctgcgc catccatctg acattcgctg
    27361 gcatgaccta acagtaggcg ctgcacacgg cgctcaactg ggttcatgct cgacagacgc
    27421 gataattttt gctcgataat ttcccattca ggcagggggt aaagcagcag gcacgggtga
    27481 tgaatgtcaa tggtgcaaac catttgaccg gcagcgttct caagcagctg ttcccgataa
    27541 cgggtaggca ctgataagcg ccctttgctg tcgagattga ctaacgttgc tccccggaac
    27601 atgccagcct caccccttat tcccactttg ccccacaatt tcccactgaa aggagtttac
    27661 ggagcggagg aaaagcttgt caagccagtc acaaggcata ccgaggtaaa aaacccgatg
    27721 tttacggcta atatcagcgt tgtttatttt acgtccaacg cgcgaagcaa attaacgtta
    27781 tgaatagctg taagaaaaaa tccctcatcg tcatcactgt tttaaaatga ctcactattt
    27841 tccacagcaa atataaagtg tcagtttgcg acgcgagcgg cattttagga catatcttcc
    27901 ccggttaaca gtccttgtta cgtctgtgtg gcgcttgatg ccagcgggtt tgcgcaaaag
    27961 caaagaaaag tgtttgttaa atcggcaatt catcgccgca tgtaacaaaa taatgcaaaa
    28021 acgcgctgtc gatatcactt aattattgtg aatttcagct ggtgagttaa ttaacgggcg
    28081 ggtttaaaac catttatcaa taatagtcag gaatagtctt atttacttta agcatattga
    28141 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
    28201 ggcttagcta cggctgagca caccgcggcg atagagatta cgtttaatgc gcgttaaacc
    28261 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
    28321 gacatcgcgg tgacgttgag ccactgccag caccgggcac tgtaagaagt cgagcagttc
    28381 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttaccatcgc gacgtagcgt
    28441 gacatccatc accccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcag
    28501 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
    28561 atacaggaaa tgcacttcgc gcggatcatc tttccaggca gtacggaaac cttgttcacg
    28621 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
    28681 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
    28741 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggg cgttagccca
    28801 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
    28861 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
    28921 aatcagcagt tgataacccc gttgccgcgc ctggcgctca agatagttag cgatgcgggt
    28981 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
    29041 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
    29101 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
    29161 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
    29221 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
    29281 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
    29341 agcccagcgt gacatgttgg gctgcaaatt gcacactgag atcattctca acgcattatt
    29401 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccacc
    29461 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
    29521 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
    29581 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
    29641 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
    29701 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
    29761 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
    29821 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
    29881 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
    29941 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
    30001 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
    30061 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
    30121 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
    30181 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
    30241 gaatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
    30301 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgagg ccaccggaat
    30361 tgatccagcg acgcggtttg tcgaatggat aataaagtgc agcaaacatc tggtgctgcc
    30421 cgacatcgga cgtcacgtaa gcgtctccct tcgtcaaccg ccaaagagtc tcgatcaccg
    30481 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgacga gcgcgccact
    30541 gttcaatttg ctgccaccag tcgcggatct catccagtgg ttgatgggcg gattcttgcg
    30601 acaagagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
    30661 cagtcacggt tttagaaatg gaaataggat caatatcgat atgcagaaca gtggcatttg
    30721 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
    30781 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
    30841 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
    30901 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
    30961 caccgcctac gtagacaacc ggtttttttg ccactaccag cgtttgcaga gcacgcttaa
    31021 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactccggcc
    31081 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgactactg
    31141 gtccaggacg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
    31201 ccgtttgctt aaccagaaaa ctgtgtttaa ccactggtcg cgaaatcccc accatgtcgc
    31261 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
    31321 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
    31381 ccgacgttac cagcacgacg ccgacttccc cggtcgcgcg cgccagacca tcggccatat
    31441 gcactgccgc ctgctcatga cgaactaaaa catgatcgat accacccacg gtatgcaatg
    31501 catcgtaaat atcaaggact gcgcctccag gataaccgaa tacttgttta acgccctgat
    31561 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
    31621 tttgatggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
    31681 ccagagacat aaacgcaggg taataagaat aaacaggagg aaagatgaaa agaaacgata
    31741 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
    31801 caatttctct atttttttaa ttaatcctaa aatagagagc ccacacattc agaataaaat
    31861 tctacattca ataaaaaatg cagaataaac cagacattca tgtctgacct attctgcatt
    31921 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
    31981 cccggcagct tcatgccatg agagataaca ggttctgctg ttttgtttta acggcagcgg
    32041 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
    32101 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
    32161 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
    32221 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
    32281 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
    32341 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
    32401 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
    32461 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
    32521 aagatgaaat acacgttcac tactcgcggg ttcaaaacct gaaccaggca attcattttg
    32581 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
    32641 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
    32701 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatgttttg
    32761 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
    32821 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
    32881 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
    32941 tttatcat
//
LOCUS       DAJTSL010000030        32476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTSL010000030 DAJTSL010000000
VERSION     DAJTSL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.030"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            196..990
                     /gene="panB"
                     /locus_tag="OO433_001247"
     CDS             196..990
                     /gene="panB"
                     /locus_tag="OO433_001247"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6049792.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1002..1853
                     /gene="panC"
                     /locus_tag="OO433_001248"
     CDS             1002..1853
                     /gene="panC"
                     /locus_tag="OO433_001248"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6049793.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2145..3041)
                     /gene="rpnC"
                     /locus_tag="OO433_001249"
     CDS             complement(2145..3041)
                     /gene="rpnC"
                     /locus_tag="OO433_001249"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6049794.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQGKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3315..3695
                     /gene="panD"
                     /locus_tag="OO433_001250"
     CDS             3315..3695
                     /gene="panD"
                     /locus_tag="OO433_001250"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6049795.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3699..4899)
                     /locus_tag="OO433_001251"
                     /pseudo
     CDS             complement(3699..4899)
                     /locus_tag="OO433_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727225.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(4994..5434)
                     /locus_tag="OO433_001252"
     CDS             complement(4994..5434)
                     /locus_tag="OO433_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6049796.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5539..6309)
                     /locus_tag="OO433_001253"
     CDS             complement(5539..6309)
                     /locus_tag="OO433_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6049797.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLATTFGVLVVFIVVFYLICWSLIQRGRGLRS"
     gene            complement(6306..7232)
                     /locus_tag="OO433_001254"
     CDS             complement(6306..7232)
                     /locus_tag="OO433_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6049798.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKLETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7341..8003
                     /gene="can"
                     /locus_tag="OO433_001255"
     CDS             7341..8003
                     /gene="can"
                     /locus_tag="OO433_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6049799.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPHFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8044..8580)
                     /gene="hpt"
                     /locus_tag="OO433_001256"
     CDS             complement(8044..8580)
                     /gene="hpt"
                     /locus_tag="OO433_001256"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6049800.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8786..11176
                     /gene="gcd"
                     /locus_tag="OO433_001257"
     CDS             8786..11176
                     /gene="gcd"
                     /locus_tag="OO433_001257"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6049801.1"
                     /translation="MAINNTGSRRLLVTLTVLFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPTEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPKLKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11223..12773)
                     /gene="cueO"
                     /locus_tag="OO433_001258"
     CDS             complement(11223..12773)
                     /gene="cueO"
                     /locus_tag="OO433_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6049802.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12939..13286
                     /locus_tag="OO433_001259"
     CDS             12939..13286
                     /locus_tag="OO433_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6049803.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13392..14258
                     /gene="speE"
                     /locus_tag="OO433_001260"
     CDS             13392..14258
                     /gene="speE"
                     /locus_tag="OO433_001260"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6049804.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPSGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14274..15068
                     /gene="speD"
                     /locus_tag="OO433_001261"
     CDS             14274..15068
                     /gene="speD"
                     /locus_tag="OO433_001261"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6049805.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15105..15467)
                     /locus_tag="OO433_001262"
     CDS             complement(15105..15467)
                     /locus_tag="OO433_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6049806.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15642..18239)
                     /gene="acnB"
                     /locus_tag="OO433_001263"
     CDS             complement(15642..18239)
                     /gene="acnB"
                     /locus_tag="OO433_001263"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6049807.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGLLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFMNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18594..20447
                     /locus_tag="OO433_001264"
     CDS             18594..20447
                     /locus_tag="OO433_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR6049808.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAAVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSNEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFRERVQESRPQRLNDSNRTARLNNDQRSAVRERLSERGARRLER"
     gene            complement(20518..21942)
                     /gene="lpdA"
                     /locus_tag="OO433_001265"
     CDS             complement(20518..21942)
                     /gene="lpdA"
                     /locus_tag="OO433_001265"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6049809.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22267..24159)
                     /gene="aceF"
                     /locus_tag="OO433_001266"
     CDS             complement(22267..24159)
                     /gene="aceF"
                     /locus_tag="OO433_001266"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6049810.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24174..26837)
                     /gene="aceE"
                     /locus_tag="OO433_001267"
     CDS             complement(24174..26837)
                     /gene="aceE"
                     /locus_tag="OO433_001267"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6049811.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHSI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26998..27762)
                     /gene="pdhR"
                     /locus_tag="OO433_001268"
     CDS             complement(26998..27762)
                     /gene="pdhR"
                     /locus_tag="OO433_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6049812.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28303..29676
                     /gene="aroP"
                     /locus_tag="OO433_001269"
     CDS             28303..29676
                     /gene="aroP"
                     /locus_tag="OO433_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6049813.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNSGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29719..30573)
                     /gene="ampE"
                     /locus_tag="OO433_001270"
     CDS             complement(29719..30573)
                     /gene="ampE"
                     /locus_tag="OO433_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6049814.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARVTMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGIWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30570..31121)
                     /gene="ampD"
                     /locus_tag="OO433_001271"
     CDS             complement(30570..31121)
                     /gene="ampD"
                     /locus_tag="OO433_001271"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6049815.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31209..32102
                     /gene="nadC"
                     /locus_tag="OO433_001272"
     CDS             31209..32102
                     /gene="nadC"
                     /locus_tag="OO433_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6049816.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32305..>32476
                     /locus_tag="OO433_001273"
     CDS             32305..>32476
                     /locus_tag="OO433_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6049817.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCA"
BASE COUNT         7828 a         8832 c         7999 g         7817 t
ORIGIN      
        1 ccacgctaca acaggatgga actataccaa tagaagctgg tgagtttaaa gccaccagta
       61 ctttccaggt aacctaccct taataagtcc accgcaccgc catcatctgg cggtgcggta
      121 attgataaat cattgccccc accaatgtga ctgataaaat gccaggcaac acgacatcat
      181 ttatcaggac acgttatgaa accgaccacc attgcttcac ttcagaaatg taagcaggat
      241 aaaaagcgtt tcgcgaccat caccgcttac gactacagct tcgcgaaact atttgctgat
      301 gaaggactta acgtcatgct ggtaggcgac tcgctgggca tgacggttca gggtcacgac
      361 tccaccctgc cggtgaccgt tgccgatatc gcctaccaca ctgcctccgt acgtcgtggt
      421 gcgccaaact gcctgctgtt ggctgacctg ccgtttatgg cgtatgccac gccggaacaa
      481 gcctttgaaa acgccgcaac ggttatgcgt gccggtgcca atatggtcaa aattgagggc
      541 ggtgagtggc tggtcgaaac cgtaaaaatg ctgaccgaac gtgccgttcc tgtatgtggt
      601 cacttaggtt taaccccaca gtcagtgaat attttcggtg gctacaaagt tcaggggcgc
      661 ggcgatgaag cgggcgatca actgctcagc gatgcattag ccttagaagc cgctggggca
      721 cagctgctgg tgctggaatg cgtgccggtt gaactggcaa aacgtattac cgaagcactg
      781 gcgatcccgg ttattggcat tggcgcaggc aacgtcactg acgggcagat cctcgtgatg
      841 cacgacgcct tcggcattac cggcggtcac attcctaaat tcgctaaaaa tttcctcgcc
      901 gaaacgggcg acatccgcgc ggctgtgcgg cagtatatgg ctgaagtgga gtccggcgtt
      961 tatccgggcg aagaacacag tttccattaa ggagtcacgt tgtgttaatt atcgaaaccc
     1021 tgccgctgct gcgtcagcaa attcgccgcc tgcgtatgga aggcaagcgc gtggcgctgg
     1081 tgcctactat gggtaatctg cacgatggcc atatgaagct ggtcgacgaa gccaaagccc
     1141 gcgccgatgt ggtcgtcgtc agtattttcg ttaacccgat gcagttcgac cgcccggaag
     1201 atctggcacg ttatccacgg accttgcagg aggactgcga gaagctaaac aaacgtaaag
     1261 tggatttagt tttcgcccct tcggtaaaag agatctaccc gaacggtact gaaacccata
     1321 cttacgttga cgttcccggg ctttcgacca tgctggaagg cgcgagccgt ccggggcatt
     1381 ttcgcggcgt ttcgactatc gtcagcaagc tgtttaacct ggtccagccg gacatcgcct
     1441 gcttcggtga aaaggacttt cagcaactgg cgctaatccg caaaatggtt gccgatatgg
     1501 gcttcgatat tgaaattgtc ggcgtcccga ttatgcgcgc caaagatggc cttgcgctca
     1561 gttcccgtaa tggttatctg acggcggaac aacgcaaaat tgcgcccggt ctgtacaaag
     1621 ttttaagttc aattgccgac aaattgcagg ctggcgagcg ggatcttgat gaaattattg
     1681 ccattgcagg gcaagaactg aatgaaaaag gcttccgcgc cgatgatatt cagattcgcg
     1741 atgccgacac gttgctggaa gtctctgaaa ccagcaaacg agctgtaatt ctggtagccg
     1801 cctggcttgg cgatgcccgc ctgatcgaca acaaaatcgt cgaactggcg taatgctttt
     1861 actctaaata attcaagttg caggaaggcg gcaagcgagt gacaaattcg tcaggaacga
     1921 atttgcccag ccgcaggctg gccttcggtg agagacatgg atgtctctca ttaatcccca
     1981 ggagcttaca taagtaagtg actggggtta gcgaacgcag acacagcaca tgcaatttaa
     2041 agtatgacga gtataactgg cgctacggct gatggcgcca attattaatt tgccccacgt
     2101 catggcgatg gcgtctgtaa gcaattattc ataacgcaga caacttagtt aatcagccta
     2161 tcaatctcag caagagataa atttgtcatc tctgcaacgt cttcccgaga catcccttta
     2221 ctcagaaaac gctgggcgaa ttcttgcctt tcctcctgtc ttccctgctg aatccccttc
     2281 tcaatccctt tctcttcaaa ccactgcgcc agcgtcatca tagactcccc tcccgtttcc
     2341 ctgtctctca gcacaccata aaacaaatcc gcttgttcag tatgaccgcg ttgcagcata
     2401 tagttttgca tggcaactaa ctgacttccg ctagtgtacc cttcgtcaat cagcgtgacc
     2461 agttgctcca gcaataacat taagtcgcgc tggcgaatat gtttttgcag tagttcgaga
     2521 atcgcaatcc gccgatgttg catgatttcg tcatccggtg tgatggtgat atccaccagc
     2581 gggaaaggac tgttatagac gcgtcgtgcc agctccggcg agtaaaatat atcaaaccag
     2641 cacattgaga gcggataagg tgtggcctcg ccctgataaa acagtatcgg caccaccagc
     2701 ggcagcttat cgtgatccgc ctccagatgc cggtgcatgg cagcaataga ataacgcatc
     2761 atgcgaaagg ccattttctt atccggcttg ccttggtgtt caatcacaac atgcagataa
     2821 ccgggattgc cctgcatttg cacggaatag agcacgtccg tgctgtgtcc tttcaggctc
     2881 tcttcaatga aactccccga ctctaaatga agcgtgttga ggtcacaaag ttctcgtaat
     2941 tccactggca aatgtatctc cagaaagtcg cgagccgtct ccgcgtgcat taaaaattgt
     3001 ttaaacactg catcatgcgg tgtggtactc ggtgcatcca tcttccctgc cttccgtgtt
     3061 gtcttgcgtg aaggcagtat ccagtcaagg cgtgccatcg tcagccacct ctttagtgtt
     3121 taccgagcag cgttcagaga aatttcaatt ggttacagcc gttacatggc acgcttcggc
     3181 acggtacgca aactgctgat gtaaatgccg tcagatgagt agacactaaa caaaaatcgg
     3241 gcaatactgc gtgagaattt cctcaaagca ggcatcgcct gcttcgttaa cgacagggta
     3301 gaaaggtaga agttatgatt cgcacgatgc tgcagggcaa actccaccgc gtgaaagtga
     3361 ctcatgcgga cctgcactat gaaggttctt gcgccattga ccaggatttt cttgacgcag
     3421 ccggtattct cgaaaacgaa gccattgata tctggaatgt caccaacggc aagcgtttct
     3481 ccacttatgc catcgcggca gaacgcggtt cgagaattat ttctgttaac ggtgcggcgg
     3541 cccactgcgc cagtgtcggc gatattgtca tcatcgccag cttcgttacc atgccagatg
     3601 aagaagctcg cacctggcga cccaacgtcg cctattttga aggcgacaat gaaatgaaac
     3661 gtaccgcgaa agcgattccg gtacaggttg cttgattgtt atccctgcgg ctggttactc
     3721 accagccgcg acatcgtctc cagcgaatcc gttcttaaga tataaagtcg ttttagtaac
     3781 aacggattat cccccggttt tactttgcct ttcatggttg tcaccgccag gtgaaatccg
     3841 gcatcgtttg ctgccttcac ggctttgtca ttaaatccgc caaacggata cgaaagatac
     3901 cagacatgcg gattaaattg cgccagagcg cggcgtgaac gtgcaaaatc aaacagaata
     3961 ttgtgttcac tacggctcgg taatatgggg cggcgataac catccacccg atgcaaaaaa
     4021 tgggtatgtg actggaaatc aaaaacatcg cgaatttcgt taagttcaga aacgctcata
     4081 aattgcagcg attttgggtc ccacttctgc gggtgacgtt tgatgcgcga ggtaacaata
     4141 aacgccgtcg ccttcatgcc atattgtttc aacacgggat acgcatagcg gctcaccgat
     4201 ttgaggccat catcaaaggt aatcaccacc gctcgcgcag ggagattgat cttattcttc
     4261 acgtaacctt ccagctgcac catgctcagt gtcgcgtatc ccctgtcgcg cagccaggcc
     4321 atctggttat tgaaagcgcg tactgaggtg gtcgtcgaag tatggcgaaa acgggtgttt
     4381 tcttcatcgc gcagaatatg gtgataggtt agcaccggca ggccattatc gggttgggca
     4441 tccagcgcgc tgatataggc cagtcgatcg ccaatacgga tctgatacca ggtttgattt
     4501 aacctgtctt tcagtttatg caaaatcggg tagcgcaaat tgtccgccag taccccataa
     4561 tggcgcactg cccgcactcg gcgcgttata gaccggcgta tctttccagg taattaagtt
     4621 ctgattactc agcggcttgt tgaggtcacc caaaccgtct tcaacttttt gtcgcccctg
     4681 aactggctcg agataccctt tatcgataaa ccctttacca aagccaaaat taaatgcgta
     4741 ataacttgcg gcagtgggct ccacggcaat gatttgcccg gcccgaatat tccccacggt
     4801 caccatcttg tcgccaattt gcgcccagat cgcagcattt tcgatggttt gcatataacg
     4861 ggcaggtaac gcggcactaa cgcttgcggt aaacagcatc agcaggatga ataacagctt
     4921 gtttatacat gaaaaaataa ccgagggtga aacaacgctt gtattgtagc aaaagcgcca
     4981 tcagaatcgc gggttaatac tcatacaaat tatgtttgag gacaaaaggc ggattttttt
     5041 gttgttggtg ccaaagacta ctcacctccg gcgcacccag ttcgacaata cgctcgcgaa
     5101 actctgaact ggtcatggtt tcacggcaag ccatcatctg ttcaattaac ggtaacgtga
     5161 caccagaaat cacttcacaa cgggagtgtt tatggcttaa taatgaagcc acgcggtacg
     5221 gtggcgctcc ggctatatcg gttaagaaga tgacaccctc accagagtcc gcttcatgca
     5281 gagcatcgca catcatgcgg ctgagcatat tagagcttaa tccgcgccag aaattcacgg
     5341 cccggcactg aagaagtgcc ccatgttttt tctccagcgc atccagtatc tcttgcgccc
     5401 ggtcatcgtg acaggtaatt acccaaccta acatcgcctt cctccttagc cgaaaatcag
     5461 tctatcctga ccattgttga taaagggtga tagccgtcaa aactcctctc ccccgaatcc
     5521 agaggagagg aaatagcctt agctacgcaa accacgtcca cgttggatca gcgaccaaca
     5581 gatcaaataa aacaccacaa taaagaccac cagtacgcca aaggtagtgg ccagcggcac
     5641 atcatttata ccgaggaagc cgtagcggaa accactgatc atataaacga ttgggttcag
     5701 gtgcgacagc ccttgccaga acggcggcaa caaagtcagg gagtaaaaca ccccgcccaa
     5761 atacgtgagt ggcgttaaca caaaggttgg caccaggctg atgtcatcga acgttttggc
     5821 aaacacgccg ttcagcaaac ccgcaaggga gaatagcacc gccgtgagca ccagggttaa
     5881 ggcaacgaat acccacgaat gcacctgaaa cggcacaaaa aacagtgaaa ttgccgtcac
     5941 cagaatgcca acaaacagac cacgcgccac gccaccgccg acatatccgg caataatgac
     6001 gtgagtcgga accggcgcta ccagcagctc ttcaatatta cgctggaact tggcaccaaa
     6061 aaatgatgac gcgacgttag cgtaggcatt ggtgatcacc gacatcatga tcagccccgg
     6121 tacgatgaac tgcatatagc tgaagccatg catatcgcca atacgcgaac ctatcaggtt
     6181 accgaagata ataaagtaaa gggtcatggt gatgactggc ggcaccagcg tctgcaccca
     6241 gatacgcata aagcgatgga tctctttcgc ccagatgctt tttagcgcca cccagtaaag
     6301 atgcatcatg cgcgatctcc ttgtttttca ttaaccagtg aaacaaacag ctcttccaga
     6361 cggttagctt tgttacgcat acttaatacc tgaatgccct gctcacttaa ctgcgtaaat
     6421 acgctgttga tcccctgctc acgcagcact tcaacttcca gcgtcgcggt atcgaccagt
     6481 cgatactgat agccatcgag cttcggtaac gggcttttcg gtgcgagatc gagaataaag
     6541 gtttccaatt tcagcttcgc cagcagcgcc ttcatcgagg tattttccac cagctcaccg
     6601 tgttgaataa tgccgatatt gcggcacagc atttctgctt cttccaggta gtgtgtagtg
     6661 agaatgatgg tggtgccttt gtcgtttaaa tccttcaaaa agccccacat tgagcggcga
     6721 agttcaatat ccacgcctgc ggtcggttcg tcgagaatca gtagtttagg ttcatgcatt
     6781 aacgcacggg caatcattaa acggcgcttc atcccgccag ataacatacg cgcacgttcg
     6841 ttgcgttttc cccataaatc gagttgtttg agatattttt cgctgcggat gtacgcttct
     6901 ttgcgctcca cgccgtagta ccctgcctga ttcaccacaa tttgctgcac ggtttcaaac
     6961 gggttgaagt taaattcctg cggcaccagt cccaactgac gtttagcgtt cacgacatcc
     7021 ttctcgagat cgtaaccaaa tacgctgacc cgcccggagg ttttatttac cagagagctg
     7081 ataataccga tagtggtcga tttcccggcc ccgttcggcc cgagaagcgc ataaaaatca
     7141 cccgcttcga cctgcaaatc tattccacga agcgcctgaa cgccgcctgg ataggttttt
     7201 ttaagctgtt gaagttccag tgcaatggtc ataaattttt acttacctta cgttcttaca
     7261 ctttatctgt ggtttaaatc gtcccggagt tgccctatat tagccaaacg taattatttg
     7321 gttacaggtc gttaacctcc atgaaagaca tagatacact catcagcaac aatgcactat
     7381 ggtcaaaaat gctggtggaa gaggatcccg ggttttttga gaaactggca caagcgcaaa
     7441 aaccgcactt tctatggatt ggatgttccg acagtcgcgt tcctgcagaa cgtttaaccg
     7501 gtcttgagcc gggcgaactc tttgttcacc gtaatgttgc taacctggtc attcacaccg
     7561 acctgaactg cctttccgtg gttcagtatg cagtggatgt actagaagtt gaacacatta
     7621 ttatctgtgg ccactacggt tgcggcggcg tacaagccgc agttgaaaac ccggaactgg
     7681 ggcttatcaa caactggctg ctgcatatcc gcgatatctg gttcaaacat agctcattgc
     7741 tcggcgaaat gccgcaagag cgccgtctgg ataccttgtg tgaactgaac gtcatggaac
     7801 aggtgtataa cctaggccac tccaccatta tgcaatcagc gtggaaacgc gggcagaaag
     7861 ttaccattca cggctgggcc tatggcattc acgacggctt gctgcgtgat ctggatgtta
     7921 ctgccaccaa ccgcgaaacc cttgagcaac gttaccgtca cgggatttcc aacctcaagc
     7981 tgaaacacgc caaccacaaa taaaaatgcc atgccggatg caacacatcc ggcaacttca
     8041 cacttactcg tccagcagaa tcactttgcc gatatacggc agatgacggt aacgctgtgc
     8101 gtaatcaatg ccgtaaccca ccacaaactc atccgggatc gagaaaccga taaattctac
     8161 cgggacgttc acttcacgac gggacggttt atccagcagc gtacaaatcg ccagcgactt
     8221 cggttcgcgc aggcttaaga tctcacgcac tttcgacagt gtattccccg agtcgatgat
     8281 atcttcaaca atcagcacgt ccttgccacg gatatcttca tccagatctt tgaggatttt
     8341 cacatcacgg gtggtggaca tgccgctacc gtagctggag gcggtcataa agtcgacttc
     8401 atgagatacc tgaacttcac ggcacaggtc cgccataaac ataaatgagc cacgcagcag
     8461 acccaccagc accatatcgc tgccgctgtc tttgtaacgc tcagtaatct gacgacccag
     8521 ttcggcgata cgcgctttaa tctccgcttc ggggatcatt acttctacag tatgtttcat
     8581 atctctaacc atatgattta aaaacaaatc acttcgcacg ggcaggtttt ggcaccgcaa
     8641 caaagcgcaa gacacgcagt ataccagtaa aactattcat tcgcggaatc tgttaataaa
     8701 gctcgaattg tgatgacgat cacacatgtt aaaacctata attaattgct actaaaatat
     8761 taatgaattg aaatggtgtc tctttatggc aattaacaat acaggctcgc gacgattact
     8821 cgtcacgcta acagtccttt ttgcagcgct ttgcgggctg tatctactca ttggcggagg
     8881 ctggctggtc gcgattggcg gctcctggta ctaccctatc gctggccttg tgatgctcgg
     8941 cgtcgcctgg atgctgtggc gcagtaaacg tgccgcgctt tggctatacg ccgccctgct
     9001 gctcggcacc atgatttggg gcgtctggga agttggtttc gacttctggg cgctgactcc
     9061 gcgcagcgat attctggtct tcttcggcat ctggctgatc ctgccgtttg tctggcgtcg
     9121 cctggtcatt cctgccagcg gcgcagttgc cgcactggtg gtggcactgc tgattagcgg
     9181 tagtatcctg acctgggccg gatttaacga tccgcaggaa atcaacggca ccttaagcgc
     9241 cgatgccaca cctacggaag ctatctcacc cgttgccgat caggactggc ctgcctatgg
     9301 tcgcaatcag gaaggtcaac gcttttcacc tctgaaacaa attaacgccg ataacgtcca
     9361 taatctgaaa gaagcctggg tgttccgtac tggcgatgtg aagcagccga acgatccggg
     9421 cgaaatcacc aatgaagtga cgccgattaa agtgggcgac accctttacc tgtgtaccgc
     9481 tcaccagcgc ctgtttgcgc tcgatgccgc cagcggcaaa gagaaatggc attacgatcc
     9541 taagctgaaa actaacgagt ctttccagca cgtaacctgc cgtggcgtct cttatcatga
     9601 agccaaagca gaaacggctt cgccggaagt gatggcggat tgcccgcgtc gtatcattct
     9661 tccggtcaat gatggtcgac tgattgcgat taacgctgaa aacggcaaac tgtgcgaaac
     9721 cttcgccaat aaaggcgtgc tcaatctgca aagcaatatg ccagacacca aaccgggtct
     9781 gtatgaaccg acttcgccac cgattatcac cgataaaacc atcgtgatgg caggttcagt
     9841 taccgataac ttctcaaccc gcgaaacgtc tggcgtgatc cgtggttttg atgtcaacac
     9901 cggggagctg ctgtgggctt ttgatcccgg cgcgaaagat ccgaacgcaa tcccgtctga
     9961 cgaacacacc tttaccttta actcgccaaa ctcttgggca ccagcggcct atgacgcgaa
    10021 gctggatctg gtctatctgc cgatgggcgt gaccacgccg gatatctggg gcggtaaccg
    10081 cacaccggaa caggaacgtt atgccagctc gattctggcg ctgaatgcca ctaccgggaa
    10141 actggcgtgg agctaccaga ccgttcacca cgacctgtgg gacatggatc ttccggcaca
    10201 gccgacgctg gcggacatca ccgttaatgg tcagaaagtg ccagttattt acgctccggc
    10261 gaaaaccggc aacatttttg tgctcgatcg tcgcaatggc gaactggtgg ttccggcccc
    10321 ggaaaaaccg gtgccgcaag gtgctgcgaa aggcgattac gttactccga cccagccgtt
    10381 ctctgaactg agcttccgtc cgaagaaaga tttgagcggt gcggatatgt ggggagccac
    10441 catgtttgac caactggtgt gccgcgtgat gttccaccag atgcgctatg aaggcatttt
    10501 cacgccgcca tctgaacagg gcacgctggt cttcccgggt aacctgggaa tgtttgaatg
    10561 gggcgggatt tccgttgatc caaatcgtga agtggcaatt gccaacccaa tggcactgcc
    10621 gtttgtttcg aaactgatcc cacgcggtcc gggtaacccg atggagcagc cgaaagatgc
    10681 caaaggcacg ggtacggaat ccggcattca gccacagtac ggtgtaccgt atggtgtcac
    10741 gctcaacccg ttcctctcac cgtttggtct gccgtgtaaa cagccagcat ggggttatat
    10801 ctcggcgctg gatctgaaaa ccaatgaagt ggtgtggaag aaacgtattg gtacgccgca
    10861 ggacagtatg ccgttcccga tgcctgtacc ggtgccgttc aatatgggta tgccgatgct
    10921 gggcgggcca atctccacgg cgggtaacgt gctgtttatc gccgctacgg cagataacta
    10981 cctgcgcgct tacaacatga gcaacggtga aaaactgtgg cagggtcgtt taccagcggg
    11041 tggtcaggcg acgccaatga cctatgaagt gaatggtaag cagtatgtgg tgatctccgc
    11101 aggcggtcac ggttcatttg gtacgaagat gggcgactat attgtggctt atgccctgcc
    11161 ggatgatgtg aaataaccat aatgcccgga gagatccggg catatttccg aatacggtct
    11221 ttttataccg taaaccctaa catcatcccc gtatcttcat gctccagcag atggcagtgc
    11281 gccatataag catgttcttt cggtgcgtcg tgattaaact tcaccagcac ttcactgaca
    11341 ttgccttcta ccttaacggt atctttccag cccgcgcgat gagccgctgg cggtttgcca
    11401 ttttctgaca agatacggaa ctgcgtgccg tgaatatgga acggatgcag catcatgtcg
    11461 cccacgccag agataaccca acgttcgtac tgccctttcg ccgccgcaaa catcggctta
    11521 ttcatatcaa acgcctgacc gttgatttta ttggcatggt ggaaatcgaa cttcccgccg
    11581 tggttcatat gattcatatt accgtgcccc atatggccca tcatctggct gtgatccatt
    11641 ccggccatcg cctgatcgcc atatttctcc attagcatct gcatacccat catatcgagc
    11701 atcgggtcca tagaaagttg cagcttgcgt accgtcagcc cttccagcga aggtaacgca
    11761 ggcaggctac ttaatgtgtc tggcaaagca ccggaggcac taatagcaat cggctgaatc
    11821 cgcattaccg gatgcggctt gtcaaacggc gcaatcgcca tccccatctg gctgaccggt
    11881 agcgtcacca ggtcaaaggg tttgttgtcg ttaacctcca ccagcacttc aaaacgctcg
    11941 cccatcagca ccggcagctc gctcaccttc accggttcag gtagcagacc accgtcgctg
    12001 gcaatcacat acagcgggcg attgtcgctg gtggcgaaat tgagcgagcg ggcattacag
    12061 ccattgagca aacgcaggcg cagccaacca cgcggggcag cgtgttgtgg gtagattgca
    12121 ccgttggtca gcagcgtatc gccaaaccag cccacggcgg cggtcatcac atccagttga
    12181 taatcaatct gcccatcagc gttaaatttt ttatcctgaa caatcaccgg aacatcatcg
    12241 ataccccact gttttggcag cattaatttc aggatctcgt catcttcaat taccaccagc
    12301 ccagccaacc ccatcgccac ctgtcgccca gtcttgccat gttgatgtgg atggaaccag
    12361 caagtagcgg caggttgatc aacgttcaac gtcaccgagc gcttgccacc tggcggaata
    12421 attccctgcg ggccgccgtc gacttcaccc ggtacttcca gcccgtgcca gtgcaacgtc
    12481 gtctcttccg tcagttggtt atagatatca accgttaccg ctttgccgcg ctgtaatttc
    12541 accgccggcc ccagcagatt gccgttatag ccccaggtag ttgcagtttt ctcgccaaag
    12601 gtagactgac ctgcgccaat agttaactga atgcgattac gggcatcggt cgtgagcaaa
    12661 tcagggatcg gtaacgttgg gcgttccgcc gcaaagactg cgcggctcca cagcggcaag
    12721 gctgaagcca cacccagcgc gacggaatat tttaagaaat cacgacgttg catagttatt
    12781 tccttattct taagcaggcg aaacaaaatc aaacgttgag catagtcctt ccccttacgg
    12841 gaaggtcaag ccgcagacac aatttaatcg acaataatgg tcgtcgcctc gctcgcagtg
    12901 tgctaacgtt tatcttcttt aagcccctgg tagaagcaat gaagacgttt ttcagaacag
    12961 tgttattcgg cagcctgatg gccgtttgcg caaacagtta cgcgctcagc gagtctgaag
    13021 ccgaagatat ggccgattta acggcagttt ttgtctttct gaagaacgat tgtggttacc
    13081 agaacttacc taacgggcaa attcgtcgcg cactggtctt tttcgcccag caaaaccagt
    13141 gggatctcag taattacgac accttcgaca tgaaagccct cggtgaagac agctaccgcg
    13201 atctcagcgg cattggcatt cccgtcgcta aaaagtgcaa agccttggcc cgcgattcct
    13261 taagcctgct tgcctacgtc aaataatccc tgataccttt ttgtagaaat attgaccgtg
    13321 catccgcggt caatgttagc tattatgttg cgcccttttt ttacgggtgt taacaaagga
    13381 ggtatcaacc catggccgaa aaaaaacagt ggcatgaaac gctacacgac cagtttgggc
    13441 agtactttgc ggtagataac gttctgtatc atgaaaagac cgatcaccaa gatctgatca
    13501 tttttgagaa cgctgcattt ggtcgcgtaa tggcgctgga tggcgtagta caaaccaccg
    13561 agcgcgacga gtttatctat catgagatga tgacccatgt tccgctactg gcccacggtc
    13621 acgcgaaaca tgtgctgatt atcggcggcg gcgacggtgc catgctgcgt gaagtaaccc
    13681 gacataaaaa cgttgagtca atcacgatgg tggaaatcga tgcgggtgtc gtgtcgttct
    13741 gccgtcagta tctgcccaac cataacgccg gtagctacga cgatccgcgc tttaagctgg
    13801 tgatcgacga tggcgtcaat ttcgttaatc aaaccagcca gacctttgat gtcattatct
    13861 ccgactgcac cgatcctatc ggtcccggcg aaagtctgtt cacctcagca ttttatgaag
    13921 gctgcaaacg ttgcctgaat cctagcggta tcttcgtcgc acaaaacggc gtctgctttt
    13981 tacagcagga agaagccatc gacagccatc gcaaactcag ccattacttc agcgacgttg
    14041 gcttttatca ggcggcgatc ccgacctatt acggcggtat catgactttt gcatgggcga
    14101 cagataacga cgccttacgc catctctcaa ccgaaattat tcaggcgcgt tttctcgctt
    14161 ctggcctgaa atgccgttat tacaatccgg caatccatac ggcagctttt gccttacctc
    14221 agtatctgca agatgcactg gcttcacagc cgtcctaagg agaagataag aaattgaaaa
    14281 aactgaaact gcatggcttt aataatctga ccaaaagtct gagtttttgt atttacgata
    14341 tctgctacgc caaaactgcc gaagagcgcg acggttatat tgcttatatc gatgaactct
    14401 ataatgccaa ccgtctgacc gaaatcctgt cagaaacctg ttccattatc ggggccaata
    14461 ttcttaacat cgcccgccag gattacgaac cacagggtgc cagcgtcact attctggtga
    14521 gtgaagaacc ggttgacccg aaactcatcg acaaaacaga acaccccggc ccactgccag
    14581 aaacggtcgt tgcccatctc gataaaagtc atatttgcgt acatacctac ccggaaagcc
    14641 atcctgaagg tggtttatgt accttccgcg ccgatattga agtctctacc tgcggcgtga
    14701 tttctccgct gaaggcgctg aattacctga tccaccagct tgagtccgat atcgtaacca
    14761 ttgattatcg cgtgcgcggt tttacccgcg acattaacgg tatgaagcac tttatcgacc
    14821 atgagattaa ttcgattcag aactttatgt ctgacgatat gaaggcgctg tatgacatgg
    14881 tggatgtaaa cgtctatcag gaaaatatct tccataccaa gatgttgctt aaagagttcg
    14941 accttaagca ctacatgttc cacaccaaac cggaagattt aaccgacagc gagcgccagg
    15001 aaattaccgc tgcgctgtgg aaagaaatgc gcgagattta ttacgggcgc aatatgccag
    15061 ctgtttaacg gctctggcgg agctccaggc tccgccagat ttatttactt ctgctgcacg
    15121 aaattgcggt aagccgccac gacctgcaga aaatcctcaa cgccgcatag cgacaggctt
    15181 tcttcgtcgt agtagttcat cccctcttcc atttcatcgc cagcgaattc cagttgattg
    15241 gcgcgaacca tcacctcttc accgtccatc cacagggtgt attcatgccc tgcccgttgc
    15301 caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt ccacttcatc aagcaaggcc
    15361 aggttttctt tcacctcttc attaaaccag tgcccgacca cttcatgccc catggacata
    15421 cgcaccttta ccactccggt aatatcgcgc agaaattcgt aatccatagt gtgttcctct
    15481 gctcccggca atgcgccgaa atcgcattgc gccattgatg taattatcgc agccttaccg
    15541 gagaaaaaca gcggaacggc gatggtttag aaataaaaaa tgcccgggaa tctcattcac
    15601 cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt attacaccgc agtctggaaa
    15661 atcaccccat cggctttttc ggtgtactga gaaagttggt tgaagttcag ataacgataa
    15721 gtatcaacgg cagttttatc tacttgcgca acgtaggtct ggtactcttc cggcgtcggc
    15781 agtttgccaa tcagcgccgc aacagccgcc agttctgcag aagccaggaa gacattcgcg
    15841 ccagtcccca gacggttcgg gaagttacgg gtagaggtgg aaaccaccgt cgcaccgtct
    15901 gctacacgcg cctggttacc catacacaga gaacagcccg ggatctcgat acgagccccg
    15961 ctcttaccga agacgctgta gtagccttct tcagtcaact gtgcggcgtc catacgggtt
    16021 ggcggtgcca cccacaggcg ggtcggcaac tggcctttat gcgcatccag cagtttaccc
    16081 gcagcacgga agtgaccgat gttagtcatg caagaaccga taaatacttc gtcgatcttc
    16141 tcgccctgta ccgcagacag cggacgcgcg tcatccgggt cgttcggagc acacaggatt
    16201 ggctctttaa tatccgccag atcgatgtca atcactgccg cgtattccgc atctgcatcg
    16261 gcttccagca gctcaggatt cgccagccat ttttccatgc cctgaatacg acgttccagg
    16321 gtacgacgat cgccgtaacc ttccgcgatc atccacttca gcaggacgat attagagtta
    16381 aggtattcga tgattggttc tttgttcagc ttgatagtac aaccagcggc agaacgctca
    16441 gcggacgcat cggtaagctc aaaggcctgc tcaactttca gatccggcag accttcaatt
    16501 tccagaatgc ggccagagaa gatgtttttc ttgcctttct tctcaacggt cagcagacct
    16561 tgtttgatgg catacagcgg aatggcgtgt accagatcgc gcagggtgat gcccggctgc
    16621 attttgcctt tgaagcgcac cagaacggat tccggcatat ccagcggcat tacgcctgtt
    16681 gcggctgcaa atgccaccag accagaaccc gccgggaaag agataccgat cgggaaacgg
    16741 gtatgggagt cacctccggt accgacggta tccggcagca gcatacggtt cagccaggag
    16801 tggattacgc cgtcacccgg acgcagcgac acaccgccac ggttcataat gaagtccggc
    16861 agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg ccgcggtgtg gcagaaagac
    16921 tgcatcacca ggtcagccga gaagcccagg cacgccaggt ctttcagttc atcacgggtc
    16981 atcgggccgg tagtgtcctg tgaacctaca gaagtcattt tcggttcgca gtacgcgccc
    17041 ggacgaatgc ctttcacgcc acaggcacgg cctaccattt tctgcgccag cgagaagccg
    17101 cgatcgctct cagcgacatc tttcgcctga cggaacacat cactgtgcgg cagaccaagt
    17161 gcttcacgcg ctttggtggt caggccacgc ccgataatca gcgggatacg gccgccagca
    17221 cgcacttcat caatcagcac gtcggttttc agttcgaagg tcgccagcag ttcgcctgtt
    17281 tcgtggttac gcacttcacc tttgtacggg taaacgtcaa tcacgtcgcc catgttcagg
    17341 ttagagacgt cgacttcgat tggcagtgca cccgcgtctt ccatcgtgtt aaagaagatg
    17401 ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt tgttcggcac atgtggaata
    17461 tcatcgccca taaaccacag aacggagttc gtggcggatt tacgcgatga acccgtaccc
    17521 acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc aatctgtttg
    17581 atcgggccaa caacacctgg ctggtctggc tcaatacctt cacgggcgtt tttcagcatc
    17641 gccagcgcgt gcagtgggat atccgggcgc gaccacgcat ccggtgccgg agaaaggtca
    17701 tcggtgttgg tttcgccagt gactttgaag acggtaacgg tcagtttttc agccagcgcc
    17761 gggcgattca taaaccattc ggcatccgcc caggactgca ttacctgctt cgcatattcg
    17821 ttgcctgctt tcgctttctc ttctacgtca tagaagttat cgaacatcag cagtgtgtga
    17881 gaaagtgctt tggcagcgat cggtgccagt ttggcatcat ccagcgcgtc gatcagcgga
    17941 tgaatgttgt aaccaccctg catggtgccc agcagttcga tggctttttc cggagtcagc
    18001 agaggggatt tggcttcgcc tttcgcgata gcagccagta agcctgcttt gacataggcg
    18061 gcttcatcga cgccaggggg aacacggttg gttaacagat ctaacaggaa ttcttcttcg
    18121 cccgcgggcg ggtttttcag cagctctaca agtgcggcca tttggtttgc atccaggggt
    18181 ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct tacggtattc ttctagcacg
    18241 acggttctcc tcgctctcat tgtcatagtg cggcaggcga ttctcttcac gctcctgtga
    18301 gacagcagtt tgtagggtaa atgcccagta ccgcaacggg cagaatagca ggattttgac
    18361 gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg ttaaggatgg
    18421 tctagagatt gtttccctcc gtaaaaactt cgttaattcg catggtgata atcacctttc
    18481 tcaacgaatc ccaagattag tcaaaattta aactaccgcc tctttatact cggattaaca
    18541 gcacctgcgg gtgacagttc gccgaccatt gcgatttcct tgagatccga attatgaaaa
    18601 tgactttgcc gtttaaaccc catgtgctgg cactaatttg cagtgccggg ctttgtgccg
    18661 cctctgccgg gctatatata aaaagccgca cagtggaagc ggctgtggaa acgcaatcga
    18721 cacaactggc tgtgtctgac gctgccgcgg ttacgcttcc tgcaacggtt tccgcgcctc
    18781 ccgtaacacc cgccgtcgtt aaatccgcat tcagcactgc acaaatagat caatgggtcg
    18841 cgcccgtcgc gctgtatccc gacgccctac tttcgcaggt gctgatggca tcaacctatc
    18901 cggcaaacgt tgctcaagca gtgcaatggt cgcacgataa tccacttaaa caaggcgatg
    18961 ctgctattca ggcggtatct gaccagccgt gggacgccag cgttaaatca ctggtggcct
    19021 ttccacaatt gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac ctgggcgatg
    19081 cttttctggc gcagccgcag gacgtgatgg actcggtaca gcgattgcgg caactggcgc
    19141 aacaaaccgg ttcgctgaag tcatcaaacg aacaaaaagt tattaccaca acgaagaaag
    19201 ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc atccaatacc gttttaactg
    19261 ccagccccgt cattacagag cctgcaacaa ccgtcatttc cattgagccc gccaatcctg
    19321 atgtggtcta tattcccaac tacaacccaa ccgtggttta cgggaactgg gccaatactg
    19381 cgtatccgcc ggtttatctg ccaccaccag ccggagaacc gtttattgac agctttgtgc
    19441 gcggattcgg ctatagcatg ggtgttgcta ccacgtacgc actattcagc agcatcgact
    19501 gggatgacga cgatcatgac catcatcatc atgacgatga tgattatcat caccacgatg
    19561 gcggtcatcg tgacggtaat ggctggcaac acaacggcga caacatcaat atcgacgtca
    19621 acaatttcaa ccgtatcacc ggtgagcatc ttactgataa gaatatggca tggcggcaca
    19681 atccaaacta ccgtaatggt gtgccctatc atgatcagga tatggcaaag cggtttcatc
    19741 aaaccgatgt caacggcgga atgagtgcca cgcagctacc tgctccaaca cgcgacagcc
    19801 agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca cgccgccccc gtcattacac
    19861 gagataccca acgtcaggca gcggcacagc ggtttaatga agctgaacac tatgggagct
    19921 atgacgactt ccgcgacttc agccgtcgcc aacccctgac ccagcaacaa aaggacgccg
    19981 ctcgtcagcg ttatcagtca gcttctcctg agcagcgcca ggcagttcgc gagagaatgc
    20041 agactaaccc gcagatccag cagcgaagag aggcagcgcg tgagcgcatt cagcccgcct
    20101 cgcctgagca gcgccaggca gtccgcgaga aaatgcagac taacccacag atccagcagc
    20161 gaagagacgc agcgcgtgag cgtattcagt cagcctcgcc tgagcagcgc caggtgttta
    20221 aggaaaaagt acagcagcgc ccactgaacc aacagcaacg tgataacgcc cgccagcgtg
    20281 ttcaatcagc atcacctgaa caacgtcagg tttttcggga gagagttcag gagagccgcc
    20341 cacaacgtct aaacgacagt aaccgtactg ccagattgaa taacgatcaa cggtcagcag
    20401 tacgcgaacg tctctctgag cgcggagcaa ggcgactgga aaggtaaatt acagacgtaa
    20461 aaaaagcggc gtggttagcc gcttttttaa ttgccggatg ttccggcaaa cgaaaaatta
    20521 cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta ccttcgaaca cttctgccgc
    20581 caggcccaca gactcgtgca gagtcgggtg cgcatggatg gtcagcgcga tgtcttcagc
    20641 gtcacaaccc atttcgattg ccaggccgat ttcacccagc agctcgccgc cgttggtacc
    20701 gacaatcgca ccaccgatca cacggtgaga ttctttgtcg aaaatcagct tggtcatacc
    20761 gtctgcgcag tcggaagcga tagcacgacc agaagcagcc cacgggaagg tggcggtttc
    20821 atagctgatg cctttctctt tcgcttcttt ctcagtcaga cctacccatg caacttctgg
    20881 ttcggtatag gcgatagacg ggataacttt cggatcgaag tagtgttttt taccggcgat
    20941 aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg gctgaccaac
    21001 gatatcgccg atagcaaaga tgtgcggtac gttggtacgc agctgtttgt caacgcggat
    21061 gaaaccacgg tcgtccactt ccacgccagc tttgcctgcg tcgaggtttt taccgttcgg
    21121 cacacgacca atcgctacca gcacggcgtc gtaacgctgc ggttcagcgg gtgctttttt
    21181 gccttccatc gtcacataaa taccgtcttc tttcgcttca acggcggtaa ctttggtttc
    21241 cagcatcagg ttgaatttct tgctgatacg cttggtgaag actttaacga tgtctttgtc
    21301 agctgccggg ataacctggt cgaacatttc aaccacgtca atctgtgaac ccagcgcgtg
    21361 gtatacggtg cccatttcca gaccgatgat accgccaccc attaccagca ggcgttctgg
    21421 tacttctttc agttccagcg cgtcagtgga gtcccagata cgcggatctt catgcggaat
    21481 aaacggcagt tggatcgggc gagaacccgc tgcaatgatc gcgttgtcga agttgatcac
    21541 ggttttaccg ttctcacctt caacttccag ggtgttagcc ccggtaaatt tacccagacc
    21601 gttgaccact ttgactttgc ggcctttcgc cataccagcc agaccaccgg tcagctgatt
    21661 gatcactttc tctttccagg tacgaatctt gtcaatatcg gttttcggtt cgccgaagac
    21721 gataccgtgt tcagccagcg ctttggcttc ttcgataact tttgctacgt gcagcagtgc
    21781 tttagaaggg atacagccga cgttcaggca aacaccgcca agggtgttgt aacgttctac
    21841 gattacggtt tccagaccta aatcagcgca acggaaggca gcggagtaac ctgcggggcc
    21901 tgccccaagt accacgacct gagttttgat ttcagtactc atcatgacct ctatatattt
    21961 atctccggcg gtcatacccg tcgtctttca ggccgcagcg gcgttagctt cacgccctca
    22021 ccccagtcac ttacttatgt aagcccctgg ggattctggc gcttgctacc ttgctgcacc
    22081 cagaaatcca tagggtatgt gaagtaatac cctaaccacc accgggtcgt tctatccgtc
    22141 ggtattttac aaaattgtta acaattttta aacaacaaac ggcaaccgat ttgtctattc
    22201 gctaataacc tcaatacatt catgagatta ccagaaaaaa gccggccgtt gggccggctc
    22261 ttttacttac atcaccagac ggcgaatgtc agacagcgtg ttgttaatga tggtaatgaa
    22321 acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag gagagagaaa tcggcagcat
    22381 cagacgcggc acgaactctt taccattcca caccggctcc atcgcggact tggaaacgcc
    22441 gaggatagcc acttccggcg cgttcacaat cggcgcgaag tgggtagtac ccaggccgcc
    22501 gatgctggag atggtgaagc aaccgccctg catttcgccc gcagtcagct taccgtcacg
    22561 cgctttctta gaaatagtca tcagctcgcg agacagctcg atgatgcctt tcttgttgac
    22621 gtctttgaat accggaacaa ccagaccgtt cggggtatcc accgccacac cgatgttgat
    22681 gtatttcttc agggtcagac gctgaccgtc ttccgacagc gaactattga agcgaggcat
    22741 ctgctcaaga gctgcagcaa cggctttcat gatgaagaca accggggtga tcttcacatc
    22801 cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg aacgcttcca gctcggtgat
    22861 atcggttttg tcgaagtggg taacatgcgg gatcatcacc cagttacggc tcaggttagc
    22921 accagaaatt ttctggatac ggcccagttc cacttcttcg atttcaccaa acttgctgaa
    22981 gtccaccttc ggccacggca gcatgcctgg gataccgccg ccagtcgccg ccggagctgc
    23041 ttctgcacgt ttgatagctt ctttcacgta agcctgaacg tcttcgcgca ggatacgacc
    23101 tttacggcca gtgcccttca ctttcgccag gttaacacca aactcgcgtg ccagacggcg
    23161 gatcagcgga gtcgcgtgaa cataagcgtc gttttcagca aattcagatt tgccttccgc
    23221 tttcgcagct ggtgctgctg ccggggcttc agcttttgct gccggtgccg gcgctgccgc
    23281 ttcctgtttc gcaggagctg ccgcaggcgc tgcgccttca acttcgaaga tcataatcag
    23341 cgagccagtt ttcactttat cgccaacgtt gactttcagt tccttcacga cgcctgcaaa
    23401 cggcgccgga acttccatag aagctttgtc gccttctacg gtgatcagtg actgttcagc
    23461 ggcaactttg tcgcccactt tcaccatcac ttcagtcact tcaacttcgt caccgccgat
    23521 atccggaacg ttaacttctt tcacgccagc tgctggtgca ggggccgctg ccggagccgc
    23581 ttcctgttta gccgccggag ctgccgcgcc tgcttcaccc gcgacttcga agaccataat
    23641 cagcgagccg gtagacactt tgtcacccac gttcactttg atctctttca cggtgccagc
    23701 aaacggagcc ggaacttcca tagaagcttt atcgccttct acggtgatca gcgactgttc
    23761 agcttcaact ttatcgccaa ctttcaccag gatttcggtc acttcaactt cgtcgctgcc
    23821 gatatccgga acgttaacgt cttttgccgc cgcagccgcg ggtgctgctg ccggagctgc
    23881 ttctttcttc tcttctgcct gagcaggtgc agcgtctgct gcaccgtcgg cggaatcgaa
    23941 aatcataatc agtgcgccgg tctgggtttt atcgccaaca gagactttga tctctttaac
    24001 gatacccgcc tgcggagacg gaacttccat agaggctttg tcgccttcta cggtgatcag
    24061 cgactgttcg gcttcaactt tgtcgcccac tttgaccagg atctcggtga tttcaacttc
    24121 atcagccccg atgtccggta ctttgatttc gatagccatt attcttttac ctcttacgcc
    24181 agacgcgggt taactttatc tgcatcgatg ttgaatttgg cgattgcgtc agcaaccact
    24241 ttcttatcga tttcgccacg tttagccagt tcgcccagcg ccgcaaccac gacataagaa
    24301 gcatcaactt cgaagtggtg acgcaggttc tcacggctgt cggaacgacc gaagccatca
    24361 gtacccagta cgcggtagtc gtcagccggt acgtaagtac ggacctgctc agcgaacagt
    24421 ttcatatagt cggtagatgc cactgccgga gcgtcgttca tcacctgagc gatatacgga
    24481 acgcgcggag tttccagcgg atgaagcatg ttccagcgtt cacaatcctg accatcacgc
    24541 gccagctcgg tgaaggaggt cacgctataa acgtcagaac ctacgccgta atctttcgcc
    24601 aggatctctg ccgcttcacg gacgtgacgc aggatagaac cggagcccag cagctgaact
    24661 ttacctttgc taccttcaat ggtttcgagt ttgtagatac ctttacggat accttcctca
    24721 gcaccttccg gcattgccgg catgtggtag ttttcgttca gcgtggtgat gtagtagtaa
    24781 acgttctctt gtttttcacc gtacatacgc tccagaccgt catgcataat gacagcaact
    24841 tcgtaagcgt aagccgggtc gtaagagata cagttcggaa tagtcagcga ctgaatgtgg
    24901 ctgtgaccat cttcatgctg cagaccttct ccgttcaggg tggtacgacc ggaagtaccg
    24961 ccgatcagga agccacgcgc ttgctggtcg ccagccgccc agcacagatc gccaatacgc
    25021 tggaagccga acatcgagta atagatgtag aacgggatca tcggcagatt gttggtgctg
    25081 taagaggtcg ccgctgccag ccaggaacaa cctgcgccca gctcgttgat cccttcctgc
    25141 agaatctgac ctttctcgtc ttctttatag taagcaacct gctcgcggtc ctgcggggtg
    25201 tactgctgac cgttcgggct gtaaatacca atctgacgga acagaccttc cataccgaaa
    25261 gtacgcgctt cgtcggcgat gatcggtacc agacgatctt tgatcgactt gttcttcagc
    25321 attacgttca gggcacgaac gaaagcgata gtggtagaga tctctttgct ctgctcttcc
    25381 agcagcgcgc cgaagtcttg caggctcggc agctcaagct tctcggtgaa gttcggctga
    25441 cggcttggca gataaccgtg cagtttctga cgctgtgcgt gcagataggt atgctcttca
    25501 gaaccttccg ggaaggtgat gtacggcagt ttttcgatat ctgcatcaga caccggcaca
    25561 ttgaaacggt cgcggatgtg acgcacgccg tccatgttca ttttcttaac ctggtgcgcg
    25621 atgtttttac cttcagccgc gtcgcccatg ccgtaacctt taatggaatg agcaaggatt
    25681 actgtcgctt tgcctttggt ttcctgcgct ttcttgaatg cagcgtagat tttcttcgga
    25741 tcgtgaccac cacggttcag tgcccagatc tgctcgtcag tccagtctgc aaccagtgct
    25801 gcggtttcag gatatttacc gaaaaagtgt tcacgaacgt acgcaccatc tttcgatttg
    25861 aaggtctggt agtcgccgtc aacggtttcg ttcatcagct ggatcagttt accgctggta
    25921 tctttacgca gcagttcatc ccaacggcta ccccacatca ctttgatcac gttccagcca
    25981 gcaccttcga agatgccttc cagttcgttg atgatcttgc cgttaccggt gaccgggccg
    26041 tcaagacgct gcaggttaca gttgataacg aagaccaggt tatccagttt ttcacgggta
    26101 gcgatggtga tcgcaccttt ggattccggt tcgtccattt caccgtcgcc gaggaacgcg
    26161 taaacggtct gtttagaggt atctttcagg ccacggtgtt ccagatattt caggaattta
    26221 gcctggtaaa tagcaccaat cggccccagc cccatagata cggtcgggaa ctgccagaat
    26281 tccggcatca gtttcgggtg cggataggaa gagaggccat tgccgtgaac ttcctgacgg
    26341 aagttatcca gctgctcctg agtcagacga ccttccagga aagcacgagc gtacacgccc
    26401 ggggagatgt ggccctggaa gtaaaccagg tcgccgccat cctgctcgtt gcgtgcacgg
    26461 aagaagtggt taaagcacac atcataaatg gttgcggaag actggaagga cgccatatgg
    26521 ccgcccagtt cgaggtcttt tttcgacgca cgcagcaccg tcatgatggc gttccagcgg
    26581 atagctgaac gaatacggcg ttccagttcc agattacccg gatactccgg ttgttcttca
    26641 acggggatgg tgttgatgta gttgctgata cctgtgcctg cggctacgtt tacaccgcct
    26701 ttgcgggctt cagcaagcag ttggtcgatc agatactgag cacgctcaac accttcttca
    26761 cggatgaccg attcgatcgc ctggagccag tcgcgagttt cgatcggatc cacgtcattt
    26821 gggaaacgtt ctgacatggg ttattcctta tctatctaat aacgttgagt tttctggaac
    26881 ctgtcccatt gaactctcgc cggaaagctc aataagacag gttctacgtt tagttgccgc
    26941 gcttttatat gcgcttgatt tacaacatct tctggataat ttttaccagt aaaatcacta
    27001 attctttcgt tgctccagac gacgcagaga acgctcacgg cggctctctt cacgacttct
    27061 gtcgagcaaa atttcttcga taaaggccag atggcgatgc gatgcttcgc gcgcttcttc
    27121 cggcttaccg gccataatcg cttcaaatat gcgggtgcgg tgactactca ccagcggcag
    27181 catctcgcga cgcgaataga gcaattcgaa gttctggcgg acattctggg ccaacatcgg
    27241 ctccatacac cttagcagat gaagcagaac cacattgtgg gccgcttcag tgacagcaat
    27301 ctgatactgg agtacggcgt ttgattccgc gtccagatcg ccagactgct gcgccagttc
    27361 tatggcgtga tggagttcac ggatgcgttc cttgtcttca tcggtactac gcagcgcggc
    27421 gtaataagcg gcgatacctt ccagggcgtg tcgtgtttcg agcaagtcat actgtgactc
    27481 aggatggtcg gagagcagct ccaccagcgg atcgctgaag ctttgccata ggctgctctg
    27541 gacaaaagtg ccgccaccct ggcgacgaag caacaagccc ttcgcttcga gacgttgaat
    27601 cgcctcacgc aaggagggac gggagacgtc aaactgtttt gccagttcgc gttccggtgg
    27661 gagtttttcg cccgggcgga gagtgccttc gaggatcaaa aactccagtt gctgctcaat
    27721 cacatcggag agttttggtt ggcggatttt gctgtaggcc atgagttcct gtcttaagcc
    27781 acttgccgaa gtcaattggt cttaccaatt tcatgtctgt gacgctaaag taacaaagta
    27841 ttcaccttat gtccatacag gttttgattg aaatcatgaa actgtgcaca ttttaacaac
    27901 ttgacatata taacgtttca aagttgtaac tatgcacaaa tgtagacttt acgtaggaaa
    27961 gggatttttt aacgattatt aaactataaa aatccgaatt gaaccgattc acttaccaat
    28021 tttgtgattt ttaattcaat taaaacgaat ttaaattcat tctacatatt gagaggggtt
    28081 gaggctgagc tttacaaacg gtttcttttt aagcaactca tcttcaacca tgcataaagc
    28141 gggtgcattc gctgccgcat accattattc ttgatctgac ggaagtcttt ttgtaacaat
    28201 tcaaacttct ttgatgtaaa caaattaata caacaaacgg aattgcaaac ttacacacgc
    28261 atcactgcgt agatcaaaaa aacaaccacc gcacgaggtt tcatgatgga aggtcaacag
    28321 cacggcgagc agctaaagcg cggccttaaa aaccgccata ttcagcttat cgcgctgggt
    28381 ggcgcgatag ggaccgggtt attcctgggt agcgcctccg taatacagtc cgcagggcca
    28441 gggattatcc tgggttacgc cattgctggt tttatcgcct ttctgatcat gcgtcagctg
    28501 ggtgaaatgg tggtcgaaga acctgtcgca ggctccttta gccactttgc ttataaatac
    28561 tggggcagtt ttgccggttt cgcctctggc tggaactact gggtactgta cgttttagtt
    28621 gccatggctg agctgactgc cgtgggtaaa tacattcagt tctggtatcc ggaaatcccc
    28681 acctgggttt ctgccgccgt attctttgtg gtgattaacg ccatcaacct gaccaacgtt
    28741 aaagtgtttg gcgagatgga gttctggttt gccattatca aagttatcgc tgtggtagcg
    28801 atgatcatct tcggcggctg gctgctattc agtggcaaca gcggcccgca ggcgaccgtt
    28861 agcaacctgt gggatcaggg tggtttcctg ccgcacggct tcaccgggct ggtgatgatg
    28921 atggcgatta tcatgttctc gttcggtggt ctggaactgg tggggatcac cgcagcagaa
    28981 gctgataacc cggagcaaag tataccgaaa gcaactaacc aggttatcta ccgcatcctg
    29041 attttctata ttggttcgtt agccgttctg ctctcactga tgccgtggac ccgcgttacc
    29101 gccgatacca gtccgtttgt gctgatcttc cacgagttag gcgatacctt tgtggcgaat
    29161 gcgctgaaca tcgtggtact gactgcggcg ctctccgtgt acaacagctg cgtatattgc
    29221 aacagccgta tgctgtttgg tctggcacaa cagggtaatg cgccaaaagc gctggcgtct
    29281 gtcgataaac gtggtgtacc agtaaatacc attctggtgt ctgcactggt aacggcgttg
    29341 tgcgtactga ttaactacct tgccccagag tccgctttcg gactgttaat ggcgctggtg
    29401 gtatctgcac tggtaatcaa ctgggcgatg attagcctgg cgcatatgaa attccgtcgc
    29461 gccaagcagg aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc gctgggtaac
    29521 tggatctgcc tgctgtttat ggcggcggta ctggtgatta tgctgatgac cccaggaatg
    29581 gcgatttcgg tatacctgat cccggtatgg ctgatcgtgt taggtatcgg ctatctgttt
    29641 aaagagaaaa ccgccaaagc cgtaaaagcg cattaatctc tctacgccct cacccgtaca
    29701 gggtgagggc aataatcttt acaccaacgc cccgtaaatc gtcagtagtg caatcaccac
    29761 cacgaccacg aacgaggttt tcttcgccat tgaaaccgct gccttcggcg tctccacctt
    29821 atcgacatgc ggttcacgcg ccagagagaa ctgcgccaga cgcgttaaca cctgatactg
    29881 cgaagtatgg aagtcaccca gcgaagcaaa ccaggccggt aacgctttct ccccatgacc
    29941 gatcaaggca tataccacac ccgcaagacg aaccggcacc caatccagta catgaagcac
    30001 ggcatcaatg ccggactgta aacgatgatg cggcgtctgg tatcgcgcca gccagtattg
    30061 ccatgcacgc aaaaacgcat accccatcag cgtaacgggt ccccagattc cccccacaat
    30121 caaccagaac agcggagcaa gataaaaacg aaagttaatc cacagcaatg cattttgcag
    30181 ctcacgcaaa tactcacgtt catcgcagcc tgccgggacg ccgtgaatca tggtgagttc
    30241 gccagccatc gtgacacggg catggctatc attacgtgaa gcagctgtca gataagcatg
    30301 ataatgaaga cgaactttac ctgcgccaat acacagcaaa ccaatcagca gccacaccag
    30361 cagcgtggga acgttgaaca atactccctg caatgcgcgt aacagtaaaa aagtcacgcc
    30421 catcgcaata atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca cccgccgaaa
    30481 gaacgcttca agacgatgat caagctgcca gtgctcgccc aacttaaaca ggcgctcgaa
    30541 aattaacacc agtaaggttg taaatagcgt catgttgtct ccttgctgac cagcgcacga
    30601 aaccgcgccc aatcaaatgc agggccggga tcggttttcc gatccggcgc aatatcacaa
    30661 tggcccgtca tgttattagc gatatctgga tagcgatcaa tcagtgcccg cgtaaccgcc
    30721 gcaagctgtt gatactgcgc atcggtatac gccagcgtat cggtgccttc aagctcaatc
    30781 ccaatagaaa aatcattgca gcgttcgcgc ccctgatact gagagactcc cgcatgccat
    30841 gcacgtttat cgaaaggaac atactggact atttcaccat cacggcgaat caaacagtga
    30901 gcggagacgc gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg atcaatagtt
    30961 ccagtgaata atgcgtcgat ccacggaccg ccaaactcgc ctggcggcag gctaatattg
    31021 tgcaccacca gcagggtggg tgtttcgtca tccgggcggc aatcgtaatg tggtgaggga
    31081 acgcggcgcg cgccaaccag ccacccctgt tctaacaaca tgcaggatct ccttatatgt
    31141 ggtgctaata cccggttcag agtagcatgt ttctacctta tgattcgtta gctatctgga
    31201 gttttaacat gccgcctcgc cgctataacc ctgacacccg acgtgacgag ctgctggaac
    31261 gcattaatct cgatatcccc ggcgcggtgg cccaggcgct gcgggaagat ttaggcggaa
    31321 cagtcgatgc caacaatgac attacggcaa aacttttacc ggaaaactct cgctctcatg
    31381 ccacggtgat cacccgcgag aatggcgtat tttgcggcaa acgctgggtt gaagaggtgt
    31441 ttattcaact ggcaggcgac gatgtcacca taatctggca tgtggatgac ggcgatgtca
    31501 tcaacgccaa tcaacccttg ttcgaacttg aaggcccatc ccgcgtgctg ttaacgggcg
    31561 aacgcactgc gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag gtacgccact
    31621 atgtcgaatt actggaaggc accaacacgc agttgttgga tacgcgcaaa accttacctg
    31681 gcctgcgttc agctctgaaa tacgcggtac tttgcggcgg cggagcgaat caccgtctgg
    31741 ggctttctga tgccttcctg atcaaagaaa atcatattat tgcctccggc tcagtgcgcc
    31801 aggccgtcga aaaagcgtcc tggctgcacc cggatgcgcc agtagaagtc gaagtagaga
    31861 atctggaaga acttgatgaa gccctgaaag caggagccga tatcatcatg ctggataact
    31921 tcgaaacaga acagatgcgc gaagccgtca aacgcaccaa cggcaaggcg ctactggaag
    31981 tgtctggcaa cgtcactgac aaaacactgc gcgaatttgc cgaaacgggc gtggacttta
    32041 tctccgtcgg tgcgctaact aaacacgtac aagcactcga cctttcaatg cgttttcgct
    32101 aattgctgac agatttatcc gctccatcgc gggcggataa tctccccttc cccaactttc
    32161 ttcgtaacgc ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa cctcggaagc
    32221 tgctttcccg tttcgttttt tgcccctcgc ttgtgctgat ggcgtttgcc aaagtagcgc
    32281 caaccaaatc aaggagcgaa acagatggac aagcaacgcg gttttacact tatcgaactg
    32341 atggtggtta ttggcatcat tgccatttta agcgccattg gtattcccgc ttatcaaaac
    32401 tacctgcgca aagctgcact caccgacatg ctacaaacct ttgtgcctta ccgtaccgcc
    32461 gtagagttgt gcgcgc
//
LOCUS       DAJTSL010000031        31909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTSL010000031 DAJTSL010000000
VERSION     DAJTSL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.031"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..954
                     /gene="tuf"
                     /locus_tag="OO433_001274"
     CDS             <1..954
                     /gene="tuf"
                     /locus_tag="OO433_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6049818.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            1184..1567
                     /gene="secE"
                     /locus_tag="OO433_001275"
     CDS             1184..1567
                     /gene="secE"
                     /locus_tag="OO433_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6049819.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1569..2114
                     /gene="nusG"
                     /locus_tag="OO433_001276"
     CDS             1569..2114
                     /gene="nusG"
                     /locus_tag="OO433_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6049820.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2274..2702
                     /gene="rplK"
                     /locus_tag="OO433_001277"
     CDS             2274..2702
                     /gene="rplK"
                     /locus_tag="OO433_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6049821.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGAGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2706..3410
                     /gene="rplA"
                     /locus_tag="OO433_001278"
     CDS             2706..3410
                     /gene="rplA"
                     /locus_tag="OO433_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6049822.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3823..4320
                     /gene="rplJ"
                     /locus_tag="OO433_001279"
     CDS             3823..4320
                     /gene="rplJ"
                     /locus_tag="OO433_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6049823.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4387..4752
                     /gene="rplL"
                     /locus_tag="OO433_001280"
     CDS             4387..4752
                     /gene="rplL"
                     /locus_tag="OO433_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6049824.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            5072..9100
                     /gene="rpoB"
                     /locus_tag="OO433_001281"
     CDS             5072..9100
                     /gene="rpoB"
                     /locus_tag="OO433_001281"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6049825.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            9177..13400
                     /gene="rpoC"
                     /locus_tag="OO433_001282"
     CDS             9177..13400
                     /gene="rpoC"
                     /locus_tag="OO433_001282"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6049826.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13613..14152
                     /locus_tag="OO433_001283"
     CDS             13613..14152
                     /locus_tag="OO433_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HCR6049827.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(14562..15695)
                     /gene="thiH"
                     /locus_tag="OO433_001284"
     CDS             complement(14562..15695)
                     /gene="thiH"
                     /locus_tag="OO433_001284"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6049828.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAKTEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15692..16462)
                     /gene="thiG"
                     /locus_tag="OO433_001285"
     CDS             complement(15692..16462)
                     /gene="thiG"
                     /locus_tag="OO433_001285"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6049829.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(16464..16664)
                     /gene="thiS"
                     /locus_tag="OO433_001286"
     CDS             complement(16464..16664)
                     /gene="thiS"
                     /locus_tag="OO433_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6049830.1"
                     /translation="MQILFNDQPMQCATGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(16648..17403)
                     /gene="thiF"
                     /locus_tag="OO433_001287"
                     /pseudo
     CDS             complement(16648..17403)
                     /gene="thiF"
                     /locus_tag="OO433_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            complement(17396..18031)
                     /gene="thiE"
                     /locus_tag="OO433_001288"
     CDS             complement(17396..18031)
                     /gene="thiE"
                     /locus_tag="OO433_001288"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6049831.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVISAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(18031..19926)
                     /gene="thiC"
                     /locus_tag="OO433_001289"
     CDS             complement(18031..19926)
                     /gene="thiC"
                     /locus_tag="OO433_001289"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6049832.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(20007..20107)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20160..20636)
                     /gene="rsd"
                     /locus_tag="OO433_001290"
     CDS             complement(20160..20636)
                     /gene="rsd"
                     /locus_tag="OO433_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6049833.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQKVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20731..21504
                     /gene="nudC"
                     /locus_tag="OO433_001291"
     CDS             20731..21504
                     /gene="nudC"
                     /locus_tag="OO433_001291"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6049834.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVVLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            21544..22608
                     /gene="hemE"
                     /locus_tag="OO433_001292"
     CDS             21544..22608
                     /gene="hemE"
                     /locus_tag="OO433_001292"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6049835.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKVTRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22618..23289
                     /gene="nfi"
                     /locus_tag="OO433_001293"
     CDS             22618..23289
                     /gene="nfi"
                     /locus_tag="OO433_001293"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6049836.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            23332..23922
                     /locus_tag="OO433_001294"
     CDS             23332..23922
                     /locus_tag="OO433_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6049837.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVVLSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQVGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            24109..24381
                     /gene="hupA"
                     /locus_tag="OO433_001295"
     CDS             24109..24381
                     /gene="hupA"
                     /locus_tag="OO433_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6049838.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            24394..25089
                     /locus_tag="OO433_001296"
     CDS             24394..25089
                     /locus_tag="OO433_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6049839.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(25091..25516)
                     /gene="zraP"
                     /locus_tag="OO433_001297"
     CDS             complement(25091..25516)
                     /gene="zraP"
                     /locus_tag="OO433_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6049840.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25754..27130
                     /gene="zraS"
                     /locus_tag="OO433_001298"
     CDS             25754..27130
                     /gene="zraS"
                     /locus_tag="OO433_001298"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6049841.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIVQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            27127..28451
                     /gene="zraR"
                     /locus_tag="OO433_001299"
                     /pseudo
     CDS             27127..28451
                     /gene="zraR"
                     /locus_tag="OO433_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(28448..29737)
                     /gene="purD"
                     /locus_tag="OO433_001300"
     CDS             complement(28448..29737)
                     /gene="purD"
                     /locus_tag="OO433_001300"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6049842.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLLLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29749..31338)
                     /gene="purH"
                     /locus_tag="OO433_001301"
     CDS             complement(29749..31338)
                     /gene="purH"
                     /locus_tag="OO433_001301"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6049843.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7564 a         8227 c         8711 g         7407 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccgtaccgt tggcgcgggc gttgtagcaa aagttctgag ctaattgccg
      961 ataacatttg actcaatgcg cactaaaagg gcatcatttg atgccctttt tgcacgcttt
     1021 cgtaccagaa cctggctcat cagtgatttt ctttgtcata atcattgctg agacaggctc
     1081 tgttgagggc gtataatccg aaaagctaat acgcgtttcg atttggtttg cctcgcgatc
     1141 gcggggtgaa aatgtttgta gaaaacttct gacaggttgg tttatgagtg cgaataccga
     1201 agctcaagga agcgggcgcg gcctggaagc gatgaagtgg gtcgttgtgg tggcattgct
     1261 cttggtggcg attgtcggca actaccttta tcgcgacatt atgctgccgc tgcgtgcgct
     1321 ggccgtagta attctgattg ctgcagcggg tggtgtcgcg ctgttaacga caaaaggtaa
     1381 agctaccgtt gcttttgccc gtgaagcgcg taccgaagtc cgtaaggtca tttggccgac
     1441 tcgccaggaa acattgcaca ccacgctgat tgtggctgcg gttaccgcag taatgtcact
     1501 gatcctgtgg ggactggatg gtattctggt tcgcctggta tcctttatca ctggcctgag
     1561 gttctgagat gtctgaagct cctaaaaagc gctggtacgt cgttcaggcg ttttccggtt
     1621 ttgaaggccg cgtagcaacg tcgctgcgtg agcatatcaa attacacaac atggaagatt
     1681 tgtttggtga agtcatggta ccaaccgaag aagtggttga aatccgtggc ggtcagcgtc
     1741 gcaaaagcga acgtaaattc ttccctggct acgtcctcgt tcagatggtg atgaacgacg
     1801 cgagctggca cctggtgcgc agcgtaccgc gagtgatggg cttcatcggc ggtacttccg
     1861 atcgtcctgc gccaatcagc gataaagaag tcgatgcgat tatgaaccgc ctgcagcagg
     1921 ttggtgataa gccgcgtccg aaaacgctgt ttgaaccggg tgaaatggtc cgtgttaatg
     1981 atggtccgtt cgctgacttc aacggtgttg ttgaagaagt ggattacgag aaatctcgtc
     2041 tgaaagtgtc tgtttctatc ttcggtcgtg cgaccccggt agagctggac ttcagccagg
     2101 ttgaaaaagc ctaacccagc gatcaaaaaa gcggcgattt aatcgttgca caaggcgcga
     2161 gattggaata caatttcgcg ccttttattt ttatgggcct tgcccgtaaa aacgattttt
     2221 tatatcacgg ggagcctctc agaggcgtta ttacccaact tgaggaattt ataatggcta
     2281 agaaagtaca agcctatgtc aagctgcagg ttgcagctgg tatggctaac ccgagtccgc
     2341 cagtaggtcc ggctctgggt cagcagggcg taaacatcat ggaattctgc aaagcgttca
     2401 acgcaaaaac tgattccatc gaaaaaggtc tgccgattcc ggtagtaatc accgtttacg
     2461 ctgaccgttc tttcactttc gttaccaaga ccccgccggc agcagttctg ctgaaaaaag
     2521 cggctggtat caagtctggt gccggtaagc cgaacaaaga caaagtgggt aaaatttccc
     2581 gcgctcagct gcaggaaatc gcgcagacca aagctgccga catgactggt gccgacattg
     2641 aagcgatgac tcgctccatc gaaggtactg cacgttccat gggcctggta gtggaggact
     2701 aagaaatggc taaactgacc aagcgcatgc gtgttatccg cgagaaagtt gatgcaacca
     2761 aacagtacga catcaacgaa gctatcgcac tgctgaaaga gctggcgact gctaaattcg
     2821 tagaaagcgt ggacgtagct gttaacctcg gcatcgacgc tcgtaaatct gaccagaacg
     2881 tacgtggtgc aactgtactg ccgcacggta ctggccgttc cgttcgcgta gccgtattta
     2941 cccaaggtgc aaacgctgaa gctgctaaag ctgcaggcgc agaactggta ggtatggaag
     3001 atctggctga ccagatcaag aaaggcgaaa tgaactttga cgttgttatt gcttctccgg
     3061 atgcaatgcg cgttgttggc cagctgggcc aggttctggg tccgcgcggc ctgatgccaa
     3121 acccgaaagt gggtactgta acaccgaacg ttgctgaagc ggttaaaaac gctaaagctg
     3181 gccaggttcg ttaccgtaac gacaaaaacg gcatcatcca caccaccatc ggtaaagtgg
     3241 actttgacgc tgacaaactg aaagaaaacc tggaagctct gctggttgcg ctgaaaaaag
     3301 caaaaccgac tcaggcgaaa ggcgtgtaca tcaagaaagt tagcatctcc accaccatgg
     3361 gtgcaggtgt tgcagttgac caggctggcc tgagcgcttc tgtaaactaa tgcctttacg
     3421 tgggcggtga ttttgtctac aatcttaccc ccacgtataa tgcttaatgc agacgtatat
     3481 ccgagatatt cgggttgtgg caaggcggca actgagtgag tcgccaagag catagctaac
     3541 tatgtgactg gtgcgaatga aggaagccaa cgccgccaca agttgaatag cgacggatag
     3601 aaaagatttg ttcgttggag cttggcctat ccaggcctcc gtcgaagacc gcaggagttt
     3661 cgcaagaaac ttaatcccct gcgtagacgg tgacagaacg ctaagattat tcttttatat
     3721 tctggcttgt ttctgctcac cgtaattaag acgctctctc cgtttggagg agtgaagtga
     3781 gttccagaga ttttctctgg caaacatcca ggagcaaagc taatggcttt aaatcttcaa
     3841 gacaaacaag cgattgttgc tgaagtcagc gaagtagcca aaggcgcgct gtctgcagta
     3901 gttgcggatt cccgtggcgt aactgtagat aaaatgactg aactgcgtaa agcaggtcgc
     3961 gaagctggcg tatacatgcg tgttgttcgt aacaccctgc tgcgccgtgc tgttgaaggt
     4021 actccgttcg agtgcctgaa agacgcgttt gttggtccga ccctgattgc atactctatg
     4081 gaacacccgg gcgctgctgc tcgtctgttc aaagagttcg cgaaagcgaa tgcaaaattt
     4141 gaggtcaaag ccgctgcctt tgaaggtgag ctgatcccgg cgtctcagat cgaccgcctg
     4201 gcaactctgc cgacctacga agaagcaatt gcacgcctga tggcaaccat gaaagaagct
     4261 tcggctggca aactggttcg tactctggct gctgtacgcg atgcgaaaga agctgcttaa
     4321 tcgcagttat ctttttaacg cattcgctta cgtataaact tattctgata ttcaggaaca
     4381 atttaaatgt ctatcactaa agatcaaatc attgaagcag ttgcagctat gtctgtaatg
     4441 gacgttgtag aactgatctc tgcaatggaa gaaaaattcg gtgtttccgc tgctgctgct
     4501 gtagctgtag ctgctggccc ggttgaagct gctgaagaaa aaactgaatt cgacgtaatt
     4561 ctgaaagctg ctggcgctaa caaagttgct gttatcaaag cagtacgtgg cgcaactggc
     4621 ctgggtctga aagaagctaa agacctggta gaatctgcac cggctgctct gaaagaaggc
     4681 gtgagcaaag acgacgcaga agcactgaaa aaagctctgg aagaagctgg cgctgaagtt
     4741 gaagttaaat aagccaaccc ttccggttgc agcctgagaa atcaggctga tggctggtga
     4801 ctttttagtc accagccttt ttgcgctgta aggcgccagt agcgtttcac actgtttgac
     4861 tactgctgtg cctttcaatg cttgtttcta tcgacgactt aatatactgc gacaggacgt
     4921 ccgttctgtg taaatcgcaa tgaaatggtt taagcgtgat agcaacaggc attgcggaaa
     4981 gtgttccatt ttccggtcaa caaaatagtg ttgcacaaac tgtccgctca atggacagat
     5041 gggtcgactt gtcagcgagc tgaggaaccc tatggtttac tcctataccg agaaaaaacg
     5101 tattcgtaag gattttggta aacgtccaca agttctggat gtaccttatc tcctttctat
     5161 ccagcttgac tcgtttcaga aatttatcga gcaagatcct gaagggcagt atggtctgga
     5221 agcagctttc cgttccgtat tcccgattca gagctacagc ggtaattccg agctgcaata
     5281 cgtcagctac cgccttggcg aaccggtgtt tgacgtccag gaatgtcaaa tccgtggcgt
     5341 gacctattcc gcaccgctgc gcgttaaact gcgtctggtg atctatgagc gcgaagcgcc
     5401 ggaaggcacc gtaaaagaca ttaaagaaca agaagtctac atgggcgaaa ttccgctcat
     5461 gacagacaac ggtacctttg ttatcaacgg tactgagcgt gttatcgttt cccagctgca
     5521 ccgtagtccg ggcgtcttct ttgactccga caaaggtaaa acccactctt cgggtaaagt
     5581 gctgtataac gcgcgcatca tcccttaccg tggttcctgg ctggacttcg aattcgatcc
     5641 gaaggacaac ctgttcgtac gtatcgaccg tcgccgtaaa ctgcctgcga ccatcattct
     5701 gcgcgccctg aactacacca cagagcagat cctcgacctg ttctttgaaa aagttatctt
     5761 tgaaatccgt gataacaagc tgcagatgga actggtgccg gaacgcctgc gtggtgaaac
     5821 cgcatctttt gacatcgaag ctaacggtaa agtgtacgta gaaaaaggcc gccgtatcac
     5881 tgcgcgccac attcgccagc tggaaaaaga cgacgtcaaa cttatcgaag tcccggttga
     5941 gtacatcgca ggtaaagtgg ttgctaaaga ctatattgat gagtctaccg gcgagctgat
     6001 ctgcgcagcg aacatggagc tgagcctgga tctgctggct aagctgagcc agtctggtca
     6061 caagcgtatc gaaacgctgt tcaccaacga tctggatcac ggcccatata tctctgaaac
     6121 cttacgtgtc gacccaacta acgaccgtct gagcgcactg gtagaaatct accgcatgat
     6181 gcgccctggc gagccgccga ctcgtgaagc agctgaaagc ctgttcgaga acctgttctt
     6241 ctccgaagac cgttatgact tgtctgcggt tggtcgtatg aagttcaacc gttctctgct
     6301 gcgcgaagaa atcgaaggtt ccggtatcct gagcaaagac gacatcattg atgttatgaa
     6361 aaagctcatc gatatccgta acggtaaagg cgaagtcgat gatatcgacc acctcggcaa
     6421 ccgtcgtatc cgttccgttg gcgaaatggc ggaaaaccag ttccgcgttg gcttggtacg
     6481 tgtagagcgt gcggtgaaag agcgtctgtc tctgggcgat ctggataccc tgatgcctca
     6541 ggatatgatc aacgccaagc cgatttccgc agcagtgaaa gagttcttcg gttccagcca
     6601 gctgtctcag tttatggacc agaacaaccc gctgtctgag attacgcaca aacgtcgtat
     6661 ctccgcactc ggcccaggcg gtctgacccg tgaacgtgca ggcttcgaag ttcgagacgt
     6721 acacccgact cactacggtc gcgtatgtcc aatcgaaacc cctgaaggtc cgaacatcgg
     6781 tctgatcaac tctctgtccg tgtacgcaca gactaacgaa tacggcttcc ttgagactcc
     6841 gtatcgtaaa gtgaccgacg gtgttgtaac tgacgaaatt cactacctgt ctgctatcga
     6901 agaaggcaac tacgttatcg cccaggcgaa ctccaacctg gatgaagaag gccacttcgt
     6961 agaagacctg gtaacttgcc gtagcaaagg cgaatccagc ttgttcagcc gcgaccaggt
     7021 tgactacatg gacgtatcca cccagcaggt ggtatccgtc ggtgcgtccc tgatcccgtt
     7081 cctggaacac gatgacgcca accgtgcatt gatgggtgcg aacatgcaac gtcaggccgt
     7141 tccgactctg cgcgctgata agccgctggt tggtactggt atggaacgtg ctgttgccgt
     7201 tgactccggt gtaactgcgg tagctaaacg tggtggtgtc gttcagtacg tggatgcttc
     7261 ccgtatcgtt atcaaagtta acgaagacga gatgtatccg ggtgaagcag gtatcgacat
     7321 ctacaacctg accaaataca cccgttctaa ccagaacacc tgtatcaacc agatgccgtg
     7381 tgtgtctctg ggtgaaccgg ttgaacgtgg cgacgtgctg gcagacggtc cgtccaccga
     7441 cctcggtgaa ctggcgcttg gtcagaacat gcgcgtagcg ttcatgccgt ggaatggtta
     7501 caacttcgaa gactccatcc tcgtatccga gcgtgttgtt caggaagacc gtttcaccac
     7561 catccacatt caggaactgg cgtgtgtgtc ccgtgacacc aagctggggc cggaagagat
     7621 caccgctgac atcccgaacg tgggtgaagc tgcgctctcc aaactagatg aatccggtat
     7681 cgtttacatt ggtgcggaag tgaccggtgg cgacattctg gttggtaagg taacgccgaa
     7741 aggtgaaact cagctgaccc cagaagaaaa actgctgcgt gcgatcttcg gtgagaaagc
     7801 ctctgacgtt aaagactctt ctctgcgcgt accaaacggt gtatccggta cggttatcga
     7861 cgttcaggtc tttactcgcg atggcgtaga aaaagacaaa cgtgcgctgg aaatcgaaga
     7921 aatgcagctc aaacaggcga agaaagacct gtctgaagaa ctgcagatcc tcgaagcggg
     7981 tctgttcagc cgtatccgtg ctgtgctggt agccggtggc gttgaagctg agaagctcga
     8041 caaactgccg cgcgatcgct ggctggagct gggcctgaca gacgaagaga aacaaaatca
     8101 gctggaacag ctggctgagc agtatgacga actgaaacac gagttcgaga agaaactcga
     8161 agcgaaacgc cgcaaaatca cccagggcga cgatctggca ccgggcgtgc tgaagattgt
     8221 taaggtatat ctggcggtta aacgccgtat ccagcctggt gacaagatgg caggtcgtca
     8281 cggtaacaag ggtgtaattt ctaagatcaa cccgatcgaa gatatgcctt acgatgaaaa
     8341 cggtacgccg gtagacatcg tactgaaccc gctgggcgta ccgtctcgta tgaacatcgg
     8401 tcagatcctc gaaacccacc tgggtatggc tgcgaaaggt atcggcgaca agatcaacgc
     8461 catgctgaaa cagcagcaag aagtcgcgaa actgcgcgaa ttcatccagc gtgcgtacga
     8521 tctgggcgct gacgttcgtc agaaagttga cctgagtacc ttcagcgatg aagaagttat
     8581 gcgtctggct gaaaacctgc gcaaaggtat gccaatcgca acgccggtgt tcgacggtgc
     8641 gaaagaagca gaaattaaag agctgctgaa acttggcgac ctgccgactt ccggtcagat
     8701 ccgcctgtac gatggtcgca ctggtgaaca gttcgagcgt ccggtaaccg ttggttacat
     8761 gtacatgctg aaactgaacc acctggtcga cgacaagatg cacgcgcgtt ccaccggttc
     8821 ttacagcctg gttactcagc agccgctggg tggtaaggca cagttcggtg gtcagcgttt
     8881 cggggagatg gaagtgtggg cgctggaagc atacggcgca gcatacaccc tgcaggaaat
     8941 gctcaccgtt aagtctgatg acgtgaacgg tcgtaccaag atgtataaaa acatcgtgga
     9001 cggcaaccat cagatggagc cgggcatgcc agaatccttc aacgtattgt tgaaagagat
     9061 tcgttcgctg ggtatcaaca tcgaactgga agacgagtaa ttctcgctca aacaggtcac
     9121 tgctgtcggg ttaaaacccg gcagcggatt gtgctaactc cgacgggagc aaatccgtga
     9181 aagatttatt aaagtttctg aaagcgcaga ctaaaaccga agagtttgat gcgatcaaaa
     9241 ttgctctggc ttcgccagac atgatccgtt catggtcttt cggtgaagtt aaaaagccgg
     9301 aaaccatcaa ctaccgtacg ttcaaaccag aacgtgacgg ccttttctgc gcccgtatct
     9361 ttgggccggt aaaagattac gagtgcctgt gcggtaagta caagcgcctg aaacaccgtg
     9421 gcgtcatctg tgagaagtgc ggcgttgaag tgacccagac taaagtacgc cgtgagcgta
     9481 tgggccacat cgaactggct tccccgactg cgcacatctg gttcctgaaa tcgctgccgt
     9541 cccgtatcgg tctgctgctc gatatgccgc tgcgcgatat cgaacgcgta ctgtactttg
     9601 aatcctatgt ggttatcgaa ggcggtatga ccaacctgga acgtcagcag atcctgactg
     9661 aagagcagta tctggacgcg ctggaagagt tcggtgacga attcgacgcg aagatgggtg
     9721 cggaagcaat ccaggctctg ctgaagagca tggatctgga gcaagagtgc gaacagctgc
     9781 gcgaagagct gaacgaaacc aactccgaaa ccaagcgtaa aaagctgacc aagcgtatca
     9841 aactgctgga agcgttcgtt cagtctggta acaaaccaga gtggatgatc ctgaccgttc
     9901 tgccggtact gccgccagat ctgcgtccgc tggttccgct ggatggtggt cgtttcgcga
     9961 cttctgacct gaacgatctg tatcgtcgcg tcattaaccg taacaaccgt ctgaaacgtc
    10021 tgctggatct ggctgcgccg gacatcatcg tacgtaacga aaaacgtatg ctgcaggaag
    10081 cggtagacgc cctgctggat aacggtcgtc gcggtcgtgc gatcaccggt tctaacaagc
    10141 gtcctctgaa atctttggcc gacatgatca aaggtaaaca gggtcgtttc cgtcagaacc
    10201 tgctcggtaa gcgtgttgac tactccggtc gttctgtaat caccgtaggt ccatacctgc
    10261 gtctgcatca gtgcggtctg ccgaagaaaa tggcgctgga gctgttcaaa ccgttcatct
    10321 acggcaagct ggaactgcgt ggtcttgcta ccaccattaa agctgcgaag aaaatggttg
    10381 agcgcgaaga agctgtcgtt tgggatatcc tggacgaagt tatccgcgaa cacccggtac
    10441 tgctgaaccg tgcaccgact ctgcaccgtc tgggtatcca ggcatttgaa ccggtactga
    10501 tcgaaggtaa agctatccag ctgcacccgc tggtttgtgc ggcatataac gccgacttcg
    10561 atggtgacca gatggctgtt cacgtaccgc tgacgctgga agcccagctg gaagcgcgtg
    10621 cgctgatgat gtctaccaac aacatcctgt ccccggcgaa cggcgaacca atcatcgttc
    10681 cgtctcagga cgttgttctg ggtctgtact atatgacccg tgactgtgtt aacgccaaag
    10741 gcgaaggcat ggtgctgact ggcccgaaag aagcagaacg tctgtatcgc tctggtctgg
    10801 cttctctgca tgcgcgcgtt aaagtgcgta tcaccgagta tgaaaaagat gcaaacggtg
    10861 aattagtagc gaaaaccagc ctgaaagaca cgactgttgg ccgtgccatt ctgtggatga
    10921 ttgtaccgaa aggtctgcct tactccatcg tcaaccaggc gctgggtaaa aaagcaatct
    10981 ccaaaatgct gaacacctgc taccgcattc tcggtctgaa accgaccgtt atttttgcgg
    11041 accagatcat gtacaccggc ttcgcctatg cagcgcgttc tggtgcatct gttggcatcg
    11101 atgacatggt catcccggag aagaaacacg aaatcatctc cgaggcagaa gcagaagttg
    11161 ctgaaattca ggagcagttc cagtctggtc tggtaactgc gggcgaacgc tacaacaaag
    11221 ttatcgatat ctgggctgcg gcgaacgatc gtgtatccaa agcgatgatg gataacctgc
    11281 aaactgaaac cgtgattaac cgtgacggtc aggaagagaa gcaggtttcc ttcaacagca
    11341 tctacatgat ggccgactcc ggtgcgcgtg gttctgcggc acagattcgt cagcttgctg
    11401 gtatgcgtgg tctgatggcg aagccggatg gctccatcat cgaaacgcca atcaccgcga
    11461 acttccgtga aggtctgaac gtactccagt acttcatctc cacccacggt gctcgtaaag
    11521 gtctggcgga taccgcactg aaaactgcaa actccggtta cctgactcgt cgtctggttg
    11581 acgtggcgca ggacctggtg gttaccgaag acgattgtgg tacccatgaa ggtatcatga
    11641 tgactccggt tatcgaaggt ggtgatgtta aagagccgct gcgcgatcgc gtactgggtc
    11701 gtgtaactgc tgaagacgtt ctgaagccgg gtactgctga tatcctcgtt ccgcgcaaca
    11761 cgctgctgca cgaacagtgg tgtgacctgc tggaagagaa ctctgtcgac gcggttaaag
    11821 tacgttctgt tgtatcttgt gacaccgact ttggtgtatg tgcgcactgc tacggtcgtg
    11881 acctggcgcg tggccacatc atcaacaagg gtgaagcaat cggtgttatc gcggcacagt
    11941 ccatcggtga accgggtaca cagctgacca tgcgtacgtt ccacatcggt ggtgcggcat
    12001 ctcgtgcggc tgctgaatcc agcatccagg tgaaaaacaa aggtagcatc aagctcagca
    12061 acgtgaagtc ggttgtgaac tccagcggta aactggttat cacttcccgt aatactgaac
    12121 tgaaactgat cgacgaattc ggtcgtacca aagaaagcta caaagtacct tacggtgcgg
    12181 tactggcgaa aggcgatggc gaacaggttg ctggcggcga aaccgttgca aactgggacc
    12241 cgcacaccat gccggttatc accgaagtaa gcggttttgt acgctttact gacatgatcg
    12301 acggtcagac cattactcgt cagacagacg aattgaccgg tctgtcttcg ctggtggttc
    12361 tggattccgc agaacgtacc gcaggtggta aagatctgcg tccggcactg aaaatcgttg
    12421 atgctcaggg taacgacgtt ctgattccgg gtaccgatat gcctgcgcag tacttcctgc
    12481 cgggtaaagc gattgttcag ctggaagatg gcgtacagat cagctctggt gacaccctgg
    12541 cgcgtattcc gcaggaatcc ggcggtacca aggacatcac cggtggtctg ccgcgcgttg
    12601 cggacctgtt cgaagcacgt cgtccgaaag agccggcaat cctggctgaa atcagcggta
    12661 tcgtttcctt cggtaaagaa accaaaggta aacgtcgtct tgttatcacc ccggtagacg
    12721 gtagcgatcc gtacgaagag atgattccga aatggcgtca gctcaacgtg ttcgaaggtg
    12781 aacgtgtaga acgtggtgac gtaatttccg acggtccgga agcgccgcac gacattctgc
    12841 gtctgcgtgg tgttcatgct gttactcgtt acatcgttaa cgaagtacag gacgtatacc
    12901 gtctgcaggg cgttaagatt aacgataaac acatcgaagt tatcgttcgt cagatgctgc
    12961 gtaaagctac catcgttaac gcgggcagct ccgacttcct ggaaggcgaa caggttgaat
    13021 actctcgcgt caagatcgca aaccgcgaac tggaagcgaa cggcaaagtg ggtgcaactt
    13081 actcccgcga tctgctgggt atcaccaaag cgtctctggc aaccgagtcc ttcatctccg
    13141 cggcatcgtt ccaggagacc actcgcgtgc tgaccgaagc agccgttgcg ggcaaacgcg
    13201 acgaactgcg cggcctgaaa gagaacgtta tcgtgggtcg tctgatcccg gcaggtaccg
    13261 gttacgcgta ccaccaggat cgtatgcgtc gccgtgctgc gggtgaagct ccggctgcac
    13321 cgcaggtgac tgcagaagac gcatctgcca gcctggcaga actgctgaac gcaggtctgg
    13381 gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg cttcggcggg
    13441 tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag gaatttctga
    13501 atactcataa tcaatgtaga gatgactaat atcctgaaac tgactgaact aattgagtca
    13561 aactcggcaa ggattcgata ctattcctgt gtaactttct taaggaacga gaatgaaaca
    13621 ggaagtggaa aagtggcgac cttttggaca tccggatggt gatattcgtg atttatcatt
    13681 tcttgatgct catcaggctg tctacgttca gcatcatgag ggcaaagagc ctttagagta
    13741 tcgcttttgg gttacctact ctcttcactg cttcacaaaa gattatgaac atcagacgaa
    13801 cgaagaaaaa caatcgttaa tgtaccacgc gcctaaagaa tctcgtccct tctgccagca
    13861 ccgttataac ttagcgcgca cacacttaaa aagaactatt ttggcgctgc cagaaagcaa
    13921 cgttattcat gccgggtatg gtagctatgc cgtgattgag gtggacttag acggaggaga
    13981 taaggcattt tactttgttg cgttcagggc tttcagggaa aagaaaaaac tccgtttgca
    14041 tgtaactagc gcttatccca tttctgaaaa acagaaaggt aaatcagtga aatttttcac
    14101 cattgcctac aacttattga gaaataagca gcttcctcag ccctcaaaat aacaaaaccc
    14161 accttaaggt gggtttcgcc agagaattat ctctggtatt cagaacgcca ttaccggact
    14221 ttgccttgac cttgcgataa tcgcaggttg cgggatgtct gaatttcttc agtctgctgc
    14281 atcctggaag atgagaacat gtgttcttat tttcgtctct atcatagttg agtatttact
    14341 ctcttacaat cagatctctt tcattgctca acaggcgatg gcttcagact ttgcattacg
    14401 gaatttttaa gaaaggcagg gcgaaacgag gaagaagctt ttcgccctgg acaacattcc
    14461 tgctgacggc actaccataa aaaaacacaa cgctttcaac aaccttgcgt ttttcatctt
    14521 ccgtagcggc tcggaataaa tttacgttct tacattccgt ctcatagtct ttgcgaggcc
    14581 cgtcccagat agctgtccca gtctttccat accggctgca aaccctgagc ggttaacgcg
    14641 gcagcaaccg cttccggtct gcgatcgtcg tgcggtgaga actgttccaa ctcggggtga
    14701 ttatcggcat agccacctgg ctgcgttttc gagaaggcgc tgacgttatt gatcgccagc
    14761 ggaataacgc gatcgcgaaa ccacggtgat tcccgcgtgg agagtgacag ttcaatctcc
    14821 ggtgcaagca gtcggaaggc gcagatggtt tgcactaact ggcgttcatc cataatcgac
    14881 gcaggctcaa tgccgccagt acacgggcgc aggcgcggaa aggagacaga gtaacggctt
    14941 tgccagtaat gctgttgcag ccatagcaaa tgttcggcaa ccatatagca gtcaacgcgc
    15001 cagttgtcgg aaaggccaat tagcgcgccg aggcctatct tatcaatccc cgcacgcccc
    15061 agccgatccg gcgtttccag ccgccagaag aagtcctgtt ttttgccttt cagatgatgg
    15121 cgggcataag tcgcctcgtg atatgtctcc tgataaacca tcacgccatc cagaccaagc
    15181 tgctttaact cggcgtattc cgtcttcgcc agcggttgca cttccatttg tagtgaagag
    15241 aactgttcac gcagggcggg gagatgacga cgaaagtaat ccatccccac tttcgcctga
    15301 tgttcaccag tgactaatag cagatgttca aagcccatct cccgtatagc ggcgctttcc
    15361 ctggcaatat ccgcttcatc cagcgttttg cgcttgatgc gattactcat ggaaaatccg
    15421 cagtacgtgc agtcgttagc gcaaagattg gaaagataaa gcgggacgta gaaactaact
    15481 gtgttgccaa atcgctgacg ggtcagacgc tgcgcccgtt gggccagttg ttccagatag
    15541 ccactggcgg ctggcgataa cagcgccatc atatcgtcgc gggtgaattg cgaggcattt
    15601 agcgcccgct ctacgtcaac agccgttttg ccgttgatac gcaggcggat gtcatcccag
    15661 tccagttgtc gccagcgatc gctgaaggtt ttcatgccga tgcctccaga aatccagtca
    15721 gcgggctggt ggcatgagca aaatgactgc ggctgcccgg tccggactga cgtgccagca
    15781 ggcctgcttc taccgccaga cgaaatgcct tcgccatgtt gacgggatcg tccgctacgg
    15841 caatcgccgt attcactaac accgcgtcgg cccccatttc cagggcctgc gctgcatggc
    15901 tgggaacgcc gatgccagca tcgacaacca ccggaactgt ggcctgctgg ataataatct
    15961 ccagcatggc acgggtttcc agtccctgat tcgagccaat cggcgcgccg agcggcatca
    16021 ccgctgcaca gccgacttct tccagacgtt tacacaatac cggatcggcc ccgcagtaag
    16081 gcagcacgac aaatccctgt tgtaccagca tttcggcggc tttcagagtt tcgatgggat
    16141 cgggcaacag ccagcgggcg tcagggtgaa tctctaattt taaccagttt gtgcctaacg
    16201 cttcacgtgc cagatgggcg gcgaaaatgg cttcttccgc tgttttcgcc ccggaagtat
    16261 ttggcagcag ggtcacaccc gccgcgataa gcggttcgag gatagcgtcg ttgtgctggc
    16321 gcaagtcgac acgtttcatc gccagtgtca ccagctggct gccggaagcg cggatcgcct
    16381 ccaccatcag ttgtgaagaa gcgaatttcc ctgtgccggt aaacagatgt gaatcaaacg
    16441 ttttgtccgc aatacgtaac atctcaaccc cctgcaataa cctgaaaaag caggatctgg
    16501 tcgccatcct gcacgatatg ttgcgtccac tgctcacgcg ggacgatttg ctgattaatc
    16561 gccagagccg cgcccgcttg tcgttggtcg agttgctcca gtagttcgtg aacagtttgc
    16621 ccggtggcgc actgcatcgg ttgatcgtta aacaggatct gcattgcttc ctccgcatac
    16681 cgggcaacca ctggcgcggc gcaacgccag gctgcgccac tggctcgatt taccgtcgaa
    16741 cagtcggagt tctcccgcag gtgtctctat accgcttaat aacttaatgg cttccagtgc
    16801 ctgcaaagtg cccataaccc cgaccaccgg gccaaccacg cccgccgtgc ggcagttgcg
    16861 ttctggctcc tggttatctg gccacaggca gcggtaacac ccctgctccc agggcggcgt
    16921 cagtaccatc aactgaccgc caaatccgac cgcgctggcg gtgataagcg gcgtgttgag
    16981 taccacgcag gcggcattaa tctcctggcg agtcgccata ttgtcggtac agtcgagcac
    17041 cacatcggcc cgtgcaaccg catcttttaa cgcctcaccc gttaaccgtt gttgtaatgc
    17101 tgtcagttga atgtcgggat tcaactgtgt cagtcgctgt tggctgacct gcgatttcgg
    17161 gctgttgata tcttcagtgg taaagagaat ttgtcgttac agattgctta aatgcacatc
    17221 gtcgtcatct gccagtacca gcgtcccgac gccagcgccc gccaggtata gcgcagcagg
    17281 tgtacccagc ccgccaagac cgataatcag cacctggctg tcgagcagtt tttgctgccc
    17341 gtcaagagcg atatcatcga gcaggatttg gcggctataa cgcataaaat cacggtcatt
    17401 catcgccaac tcctgcaatt tccagcaact gtgccgttgc caaacgccag tctgcggctt
    17461 gagtaatggc gctgatgacg gcgatactgc cgacacccgt tgctatcacc gcaggcgcgc
    17521 gtgccagact gataccgcca atcgccacgg tgggataatc cgccagtcgc tcaacatgcc
    17581 gtgccagctg ttccagcccc tgcggtgcag aaggcatctg tttggtttgc gtcgggaaca
    17641 catgtcccag cgcgatataa gaggggcgtg ctgccagcgc gacgtcgatt tccatatcgt
    17701 catgtgtcga aacacccagc cgcaggcctg ccgcgcggat ggcattgaga tcggtggctt
    17761 gcaaatcttc ctgccccaaa tggacgccat acgcctgatg cttgatcgcg agccgccagt
    17821 aatcgttgat aaacaatcgc gcgttatagc ggcggcccag cgcaattgcc gccacgacat
    17881 cggcttccac ctcttcatcg cgccgatctt tgatgcgtag ctggagagta cgtacgcctg
    17941 catccaacag acgttcgatc cactgtacgc tgtccaccac tgggtacagt cctaaacgaa
    18001 aaggtacagg aggaaaatca ggctgataca tcacgcttcc tccttacgca ggtagatttc
    18061 tccgcctctg gcacggaagt tctccgacat atccgccatt cccatttcaa tagtttgcgt
    18121 ggcggcgtaa tcacgcactt cctggctgat tttcatcgag cagaatttcg gcccacacat
    18181 ggagcaaaaa tgggcgactt tacctgactc ttgcggcagg gtttcatcgt gataagcgcg
    18241 ggcggtaaac gggtcgaggg ccagattaaa ctggtcttcc cagcgaaatt cgaagcgggc
    18301 tttcgacatg gcgttatcgc gaatttgcgc gcccggatgc cctttcgcca ggtcagcggc
    18361 gtgggcggca atcttatagg tgataagccc ctgcttaaca tcttctttat taggcagacc
    18421 cagatgctct tttggtgtta cgtaacagag catcgcgcag ccaaaccagc caatcatcgc
    18481 cgcaccaatc cccgacgtga agtggtcata gcccggcgca atatcggtag ttagcggccc
    18541 cagagtgtaa aacggcgctt cgtggcagtg ctctaactcc tcggtcatat tgcggcggat
    18601 catctgcatc ggcacgtggc ctgggccttc aatcatcacc tgcacgtcat attcccaggc
    18661 aattttggtc agttcgccca gcgtatgcag ctcggcaaac tgcgcttcat cgttggcgtc
    18721 ctgaatagaa ccggggcgca gaccgtcgcc cagcgacagc gaaacgtcat aagcggcaca
    18781 gatttcacaa atttcgcgga agtgttgata gaggaaattt tcctgatgat gggagaggca
    18841 ccatttcgcc ataatcgaac cgccgcgaga gacgataccg gtcaggcgtt tcgcggtcat
    18901 cggcacatag cgcaacagta cgcccgcatg gatagtgaag taatccacac cttgctcggc
    18961 ctgttccagc agcgtgtcgc ggaacgcttc ccaggtaaga tcttcggcga tcccgttaac
    19021 cttctccagc gcctggtaga tcggcactgt accgatcggc accggactgt tacgcaaaat
    19081 ccactcgcgg gtttcgtgaa tatagcgacc ggtggagaga tccatcaccg tatccgctcc
    19141 ccagcgcgtg gaccatacca gcttttccac ttcttcttcg atggaagagg tgaccgccga
    19201 gttgccgata ttggcgttaa cttttaccag gaagttacgg ccaataatca tcggctccga
    19261 ttccggatga ttaatgttgg ccgggataat cgcacgtccg gcagcaactt catcacggac
    19321 aaattccgca gtgatatttt ccggcagacg tgcgccaaag ctcattcccg gatgctggtg
    19381 gcgtaaaact tcgctacgta tgcgctcgcg gcccatattc tcgcggatgg cgatgaattc
    19441 catttccggt gtgatgatgc cctggcgggc gtagtgcagt tgggtgacac ggcgtcctgc
    19501 tttggcgcgt tttggtgtta gtacgccgct aaaacgcagt tcgtcgaggc catcatctgc
    19561 cagccgcgct ttagtgtaat cggaactgcg cacggtaagt tcttcggtat cgccgcgcgc
    19621 atcgatccac ggctggcgta gttttgccag cccttgctgc acgttaatgg cgatctgcgg
    19681 atcgccatat gggccggagg tgtcgtagac cggaatcgct tcgttttctt caaactgcgg
    19741 ctgttctttg ctgccgccga ttagcgtcgg gctaagctgg atctcacgca tcggcacgcg
    19801 aacgccagag tgtgtgccag tgaggtaaat gcgttttgag ttgggaaagg cggtgccttc
    19861 cagggtgtcg ataaaatgtt gggcccgggc gcgttgttcg cggcgggtca gttttgttgc
    19921 agacatagct cattccaaaa agttaaggac gtggcttgtc agacgacgga tgaagcaaga
    19981 gacgatcgcc gcaaaggcga tgcgatagca gattaactct tgttcccttc gcaggtatta
    20041 gcctgatcag gttccgcgga tcccgaataa acggtctcag ccagttaagg cactccgaca
    20101 agaaattagc ccccacaaac ggggcgttga atgtaaatta cgcgttaaca gcgcagaact
    20161 caagcaggat gtttgacgcg ggcggcgtca agcaccagca gaatcagctt atcttccagc
    20221 acaaagcgcg cttccagcgc ctcgccgatg tcagataaaa ccttttgaaa ctcaaggtaa
    20281 ttatcatgat cgatggcggt ttccaggctg gaatcgtagt aatccataat ctgttgggta
    20341 ttggcttcga gttgcggcca aatcttcgcg gcgcgcgcga gttgcccgtt gccttccagc
    20401 ttatgaagaa tgcgctcata aatactgaaa tgtccggcag acaagtaatc gaccaggctc
    20461 tgacaaaaat catcaagggc tttttcgttt agcctcatgt acgattcttt gccaggctta
    20521 atgccaacca gattgtagta agccacgagc agatgcttac gtacatgtag ccagcgatca
    20581 accagtttgt tacttcctct gacgcgctcc gtcaggttat cgagctggtt aagcatgatt
    20641 gactccgcaa gtttgtattc aaaaactgct cagtgagaaa tgtaaaaacc atgttaaaca
    20701 tgccagtgat gcaaaggtag tgcaagagct atggatcgta taattgaaaa attagatcac
    20761 ggctggtggg tcgtcagcca tgaacaaaaa ttatggttgc cgaagggaga attgccatat
    20821 ggcgaagcgg caaatttcga tcttgtgggt cagcgcgcac tacaaatcgg cgaatggcag
    20881 ggggaacctg tttggttagt acaacagcag cggcgtcacg atatggggtc ggtacgtcag
    20941 gtcattgatc tcgatgttgt gctgtttcaa ctggccggac gaggcgtaca actggcggag
    21001 ttttaccgat cgcataaata ctgtggttac tgcgggcatg aaatgtatcc gagcaaaacc
    21061 gaatgggcga tgctgtgcag ccattgccgt gagcgttact acccgcaaat cgccccctgc
    21121 attattgttg ccatccgtcg cgatgattcg atcctcctcg cccagcatac ccgccatcgt
    21181 aacggtgtcc atacagtact tgccggattc gtcgaagtgg gcgaaaccct cgagcaggca
    21241 gtcgcgcggg aagtgatgga agagagcgga attaaagtta aaaacttgcg ttacgtgact
    21301 tctcagccgt ggccgtttcc tcagtcttta atgaccgcgt ttatggcgga atatgacagc
    21361 ggcgacatcg tgatcgaccc gaaagaattg ctcgaggcga actggtatcg ctatgacgat
    21421 ttgccgttac tcccgccgcc cggcaccgta gcgcgccgtc tgatagaaga tactgtggcg
    21481 atgtgtcggg cagagtatga gtgatgatac actgaccgcc tgacgcacta aggaacagcc
    21541 aaaatgaccg aacttaaaaa cgatcgttat ctgcgggcgc tgctgcgcca gcccgttgat
    21601 gtcactccag tatggatgat gcgccaggcg ggtcgctatc taccggaata taaagtcacg
    21661 cgcgcccagg cgggcgattt tatgtcgctg tgcaaaaacg ccgagctggc gtgcgaagtg
    21721 actttgcaac cgctgcgtcg ctacccgctg gatgcggcga tcctcttttc cgatatcctc
    21781 accgtgccgg acgcgatggg gttagggctc tattttgaag ccggagaagg tccgcgtttt
    21841 acctcgccag tcacctgcaa agccgacgtc gataagctgc caattccgga cccggaagat
    21901 gagctgggtt acgtgatgaa cgcggtgcgt accattcgtc gcgaactgaa aggcgaagtg
    21961 ccgctgattg gtttttccgg cagcccgtgg acgctggcaa cctacatggt ggaaggcggc
    22021 agcagcaaag ccttcaccgt gatcaaaaaa atgatgtatg ccgatccgca ggcgctgcac
    22081 gctctactcg ataaactggc gaaaagcgtc actttgtatc tgaatgcgca gattaaagcc
    22141 ggtgctcagg cagtgatgat tttcgacacc tggggcggtg tgcttaccgg gcgcgattat
    22201 caacagttct cgctctatta catgcataaa attgttgatg gtttactgcg tgaaaacgac
    22261 ggtcgccgcg taccggtcac gctgtttacc aaaggcggcg gacagtggct ggaagcgatg
    22321 gcagaaaccg gttgtgatgc gttgggcctc gactggacaa cggatatcgc cgatgcgcgc
    22381 cgccgtgtgg gcaataaagt cgcgttgcag ggtaatatgg atccgtcgat gctgtacgcg
    22441 ccgcctgccc gcattgaaga agaagtagcg actatacttg caggtttcgg tcacggcgaa
    22501 ggtcatgtct ttaaccttgg tcacggcatt catcaggatg tgccgccaga acatgctggc
    22561 gtgttcgtgg aggcagtgca tcgactgtct gaacaatatc accgctaagg agtgattatg
    22621 gatctcgcgt cattacgtgc tcaacaaatc gaactggctt cttctgtgat ccgcgaggat
    22681 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    22741 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    22801 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    22861 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    22921 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggtttg
    22981 atggtggatg tgccaacgat cggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    23041 ctctccagcg aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    23101 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    23161 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    23221 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    23281 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaacttaa catgttacaa
    23341 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    23401 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    23461 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    23521 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    23581 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggtgtt aagtgaactg
    23641 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    23701 atcacgaccg ttgcgatgct ggaaatgact caggttggtc gcgaaatgag cgatgaagag
    23761 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    23821 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    23881 gaggcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    23941 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    24001 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    24061 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    24121 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    24181 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    24241 gttggtttcg gtaccttcaa agtgaaccac cgcgctgagc gtactggccg caacccgcag
    24301 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    24361 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    24421 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg ctggcgctct gcttctcact
    24481 gcctgtagtc ataactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    24541 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    24601 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    24661 acgctcatca atatcaatgt ttacagcaaa ccgccggtga atattcgcgc gcgttttgac
    24721 gatcgcggtg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    24781 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    24841 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    24901 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    24961 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    25021 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    25081 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    25141 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    25201 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    25261 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    25321 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    25381 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    25441 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    25501 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    25561 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    25621 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    25681 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    25741 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    25801 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    25861 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    25921 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    25981 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    26041 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    26101 ttccccgcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    26161 tagcgcagac ggtgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    26221 tggaatgcac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    26281 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    26341 tatcctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    26401 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    26461 ggcactgggg catctggcgg caggcgttgc ccacgaaatc cgtaatccac tttcctcaat
    26521 taaagggctg gcgaaatact ttgccgaacg cgcgccagca gggggagaag cgcatcaact
    26581 ggcgcaggtg atggcgaaag aagccgaccg tttaaaccgc gtggtaagcg agttgctgga
    26641 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    26701 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    26761 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    26821 tctctatctc aatgctattc aggcgattgt tcagcatggc gtgattagcg tgacggtcag
    26881 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    26941 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    27001 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    27061 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    27121 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    27181 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    27241 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    27301 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    27361 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    27421 ggggcgctgg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    27481 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    27541 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    27601 gcgccatcgg aagccacagt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    27661 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    27721 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    27781 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    27841 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    27901 gagctcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    27961 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    28021 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    28081 atccctctgc tggctggcca ttttctgcag cgctttgccg agcgtaatcg caaggcggta
    28141 aaaggtttta cgccccaggc gatggatctg ttgattcatt acgactggcc gggaaatatt
    28201 cgtgagctgg aaaacgcggt ggaacgggca gtggtgctgc tgccggggaa tatatttccg
    28261 aacgcgagct gccgctggcg attgccagta cgccgatccc gctggggcaa agtcaggata
    28321 ttcagccgct ggtggaagtg gaaaaagagg tgattctggc ggcactggag aaaacgggcg
    28381 gcaacaaaac tgaagccgcc cgtcagttag ggatcacgcg taaaacgtta ttggcaaaac
    28441 tgtcgcgtta gttctgctcg cgttcgatag cacgccagcc gatatctttc cggcagaagc
    28501 agtcgtccca gtggatatcg gtcattaagg cataggcgcg tttctgcgct tctgccacgg
    28561 tatgacccag cgcggtgacg cacagtacgc gcccgccgtt ggtcactacc tgctcgtcat
    28621 ccgccagttt tgtgcccgcg tggaacactt tgccgcctgc cacttcttcc agcagcaggc
    28681 cgtggatcac gtcaccggtg cggtaatcac ccggatatcc acccgcagcc atcaccacgc
    28741 cgagagaagc gcgttcatcc cactcggacg ttttctcgtc cagtttgctt tcacaggccg
    28801 ccaggcagag ctcaaccaga tcggacttca tgcgcagcat aatcggctgg gtttccggat
    28861 cgccaaagcg gcagttaaat tcaataacct tcggattgcc ctgtttgtcg atcatcaggc
    28921 ccgcgtagag aaaaccggtg taggtgttgc cttccgccgc catgcctttc acggttggcc
    28981 agataatacg ttccatggtg cgctgatgaa cgtcatcggt tactaccggc gcgggggagt
    29041 aagcgcccat cccgccggtg tttggtccgg tatctttatc gcctacgcgt ttgtgatcct
    29101 ggctggtagc catcggcagc acatgctcgc cgtccaccat cacgataaag ctcgcttctt
    29161 cgccatcgag gaactcttcg ataacgatgc gatgacccgc gtcgccaaaa gcgttgcccg
    29221 ccagcatatc gtgaacagcc gcttccgctt cttccagcgt catcgccacg ataacgcctt
    29281 tcccggcagc cagaccgtcc gctttaatga cgattggcgc gcctttctca cgcagatacg
    29341 ccagcgcagg ttctacctcg gtgaagttct ggtattccgc cgtagggatc ttatggcggg
    29401 ccaggaaatc tttggtaaac gcttttgagc cttccagttg ggccgcacct gcggttgggc
    29461 cgaagatttt cagcccggcg gcgcggaagg tatcgaccac gcctttcacc agcggcgctt
    29521 ccgggccgac gatggtcaga tcaatctttt cgttttgtgc gaaatccaac agcgccggga
    29581 tatcggtcac gccaatagca acgttttgca gcgcgggttc cagcgcagtg cctgcattac
    29641 ccggagcaac aaaaacagtc tcaaccagcg gcgactgggc cgctttccag gccagcgcgt
    29701 gctcgcgccc gccgttacca atcactaata ctttcatcta ttgctccatt aatggcggaa
    29761 gtggcgcatg tcggtgaaga gcatcgcaat accgtgctcg tcggcggcgg caatcacttc
    29821 gtcatcacgg atggaaccgc caggctggat tacgcaggtc acgcccgcag cggcggcggc
    29881 atcaataccg tcgcggaacg ggaagaacgc gtcagaagcc atcgaggaac ctttcacttc
    29941 caggccttca tcggcagctt taataccggc gattttcgcg gagtacacgc ggctcatctg
    30001 gcccgcgcca ataccgatgg tcatattgtt tttggcatag acgatagcat tggatttcac
    30061 gaacttcgcc actttccagc agaacagcgc atcgcgcagt tcctgttcgg tcggctgacg
    30121 ttgggtgacg acgcgcagtt cttccgcacc gaccatcccc aggtcacgat cctgaaccag
    30181 cagaccgccg ttcacgcgtt tgaaatcaag acccggaaca cgctcgcccc actgaccgca
    30241 ggtcagaacg cgtacgttct gcttggcggc ggtgattttc agggcttctt cgctggcgga
    30301 aggcgcaata atcacttcga caaactgacg agaaatgatg gcctgtgcgg tttccgcatc
    30361 tagctcgcgg ttaaaggcaa tgatgccgcc gaatgcggag gttgggtcgg ttttgtacgc
    30421 gcgatcgtaa gcatcaagaa tggaattgcc gatagctaca ccgcaagggt tggcgtgctt
    30481 cacaatcaca catgccggct cggcgaactc tttcacgcac tccagcgccg catcggtatc
    30541 ggcgatgttg ttataagaga gggctttacc ctgaacctgg gttgcggtag caacggaggc
    30601 ttctttcaca ttctcttcta tatagaaggc agcctgctgg tggctgttct cgccgtaacg
    30661 catatcctgc ttcttaatga agttcaggtt cagcgtgcgt gggaagcgac cggcggcttc
    30721 tttgctttca ccgtggtacg ccggaaccat gctgccgaag tagttggcaa tcatgctgtc
    30781 gtaggcggca gtgtgttcga aggctttgat ggcgaggtcg aaacgggttg caagcgtcag
    30841 cgatccttcg ttgtcatcca tctctttaat aatggcgtcg tagtcgctgc tcttcaccac
    30901 gattgcgaca tctttatggt tcttagcggc ggagcgcacc atggtcgggc cgccgatatc
    30961 gatgttctca accgcatctt ccagcgagca gccttcacga gcaacagtct gggcgaacgg
    31021 atacaggtta acaaccacca tatcgatagg ctggatctga tgttcttcca taatggcatc
    31081 gtcctggccg cgacggccca gaatgccacc atgtactttc ggatgcaggg tcttcacgcg
    31141 tccatccatc atctccggga aaccggtgta atcggaaact tcggttaccg gcagaccttt
    31201 ttctgctaac agacgggcag tgccccctgt agacagcagc tccacaccgc gtgcggaaag
    31261 tgcctgggcg aattcgacga taccggcttt gtcagaaaca ctgagcagag cgcggcggac
    31321 tggacgacgt tgttgcatgg taaatcccct ggatttgact attacagaga gcgttagctg
    31381 aatttttcgc gaaaaactca gctaacgccc ctaacggggc atccttattt ttcgcccgca
    31441 ttgtaacgaa aacgtttgcg caacgctcgc gaatttttct ctttcaatgg tgatcacaat
    31501 tttgacagtg gctaccgtgg gaaaaataca gaaattacat tgatgattgt ggataactct
    31561 gtgcgtaaaa aggtataaag cggggttttg ctggggaatg cagcagtcag tcatttttct
    31621 gcaatttttc tattgcggcc tgcggagaac tccctataat gaacctccat cgacacggcg
    31681 gatgtgaatc acttcacaca aacagccggt tcggttgaag agaaaaatcc tgaaattcag
    31741 ggttgactct gaaagaggaa agcgtaatat acgccacact cacgacagtg cgctaaagct
    31801 cgtcgcaact gctctttaac aatttatcag acaatctgtg tgggtactcg aagatacgga
    31861 ttcttaacgt cgcaagacga taaatgaata ccaagtctca agagtgaac
//
LOCUS       DAJTSL010000032        31375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTSL010000032 DAJTSL010000000
VERSION     DAJTSL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.032"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(18..3299)
                     /locus_tag="OO433_001302"
     CDS             complement(18..3299)
                     /locus_tag="OO433_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR6049844.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISCYKNVPVTTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLVIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLLDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            3417..4610
                     /locus_tag="OO433_001303"
     CDS             3417..4610
                     /locus_tag="OO433_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6049845.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4674..5927)
                     /gene="glyA"
                     /locus_tag="OO433_001304"
     CDS             complement(4674..5927)
                     /gene="glyA"
                     /locus_tag="OO433_001304"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6049846.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6255..7445
                     /gene="hmpA"
                     /locus_tag="OO433_001305"
     CDS             6255..7445
                     /gene="hmpA"
                     /locus_tag="OO433_001305"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6049847.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISSGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIYYECFGP
                     HKVL"
     gene            complement(7490..7828)
                     /gene="glnB"
                     /locus_tag="OO433_001306"
     CDS             complement(7490..7828)
                     /gene="glnB"
                     /locus_tag="OO433_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6049848.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7889..9223)
                     /gene="glrR"
                     /locus_tag="OO433_001307"
     CDS             complement(7889..9223)
                     /gene="glrR"
                     /locus_tag="OO433_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6049849.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9213..9926)
                     /gene="qseG"
                     /locus_tag="OO433_001308"
     CDS             complement(9213..9926)
                     /gene="qseG"
                     /locus_tag="OO433_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6049850.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGCWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10091..11518)
                     /gene="qseE"
                     /locus_tag="OO433_001309"
     CDS             complement(10091..11518)
                     /gene="qseE"
                     /locus_tag="OO433_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6049851.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12076..15963)
                     /gene="purL"
                     /locus_tag="OO433_001310"
                     /gene_synonym="purI"
     CDS             complement(12076..15963)
                     /gene="purL"
                     /locus_tag="OO433_001310"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6049852.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRILGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRILHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16224..17780
                     /gene="mltF"
                     /locus_tag="OO433_001311"
     CDS             16224..17780
                     /gene="mltF"
                     /locus_tag="OO433_001311"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6049853.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLCNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17777..18313)
                     /gene="tadA"
                     /locus_tag="OO433_001312"
     CDS             complement(17777..18313)
                     /gene="tadA"
                     /locus_tag="OO433_001312"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6049854.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18338..18973)
                     /gene="yfhb"
                     /locus_tag="OO433_001313"
     CDS             complement(18338..18973)
                     /gene="yfhb"
                     /locus_tag="OO433_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6049855.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDKPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19182..20030
                     /locus_tag="OO433_001314"
     CDS             19182..20030
                     /locus_tag="OO433_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6049856.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            20086..20346
                     /gene="yfhL"
                     /locus_tag="OO433_001315"
     CDS             20086..20346
                     /gene="yfhL"
                     /locus_tag="OO433_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6049857.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(20540..20620)
                     /gene="shoB"
                     /locus_tag="OO433_001316"
     CDS             complement(20540..20620)
                     /gene="shoB"
                     /locus_tag="OO433_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6049858.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(21040..21420)
                     /locus_tag="OO433_001317"
     CDS             complement(21040..21420)
                     /locus_tag="OO433_001317"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6049859.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21420..22151)
                     /gene="pdxJ"
                     /locus_tag="OO433_001318"
     CDS             complement(21420..22151)
                     /gene="pdxJ"
                     /locus_tag="OO433_001318"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6049860.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22163..22891)
                     /gene="recO"
                     /locus_tag="OO433_001319"
     CDS             complement(22163..22891)
                     /gene="recO"
                     /locus_tag="OO433_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6049861.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTYCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(22903..23808)
                     /gene="era"
                     /locus_tag="OO433_001320"
     CDS             complement(22903..23808)
                     /gene="era"
                     /locus_tag="OO433_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6049862.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(23805..24485)
                     /gene="rnc"
                     /locus_tag="OO433_001321"
     CDS             complement(23805..24485)
                     /gene="rnc"
                     /locus_tag="OO433_001321"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6049863.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(24758..25732)
                     /gene="lepB"
                     /locus_tag="OO433_001322"
     CDS             complement(24758..25732)
                     /gene="lepB"
                     /locus_tag="OO433_001322"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6049864.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(25748..27547)
                     /gene="lepA"
                     /locus_tag="OO433_001323"
     CDS             complement(25748..27547)
                     /gene="lepA"
                     /locus_tag="OO433_001323"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6049865.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(27745..28224)
                     /gene="rseC"
                     /locus_tag="OO433_001324"
     CDS             complement(27745..28224)
                     /gene="rseC"
                     /locus_tag="OO433_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6049866.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28221..29177)
                     /gene="rseB"
                     /locus_tag="OO433_001325"
     CDS             complement(28221..29177)
                     /gene="rseB"
                     /locus_tag="OO433_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6049867.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGDEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29177..29827)
                     /gene="rseA"
                     /locus_tag="OO433_001326"
     CDS             complement(29177..29827)
                     /gene="rseA"
                     /locus_tag="OO433_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6049868.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(29860..30435)
                     /gene="rpoE"
                     /locus_tag="OO433_001327"
     CDS             complement(29860..30435)
                     /gene="rpoE"
                     /locus_tag="OO433_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6049869.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30432..30587)
                     /gene="rseD"
                     /locus_tag="OO433_001328"
     CDS             complement(30432..30587)
                     /gene="rseD"
                     /locus_tag="OO433_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6049870.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            30843..>31375
                     /locus_tag="OO433_001329"
     CDS             30843..>31375
                     /locus_tag="OO433_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6049871.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRR"
BASE COUNT         7503 a         8402 c         7919 g         7551 t
ORIGIN      
        1 attacagtta atagggttta gtgaacgtag ttaaaaataa aaggcatcac agtggtgaat
       61 aatgccgctt tcggccaggc cggattaatc gccgtcaatt cagcgcgagc agactcatat
      121 tgcgcaacct ccccgagtcc cgcggacgcc agcatcgcca ccatcaggca tttttcttta
      181 tgctgctgtt gtaaatcgcc atacagcgat aacaggtcag gttgtgaaac agcaaagaaa
      241 tccgcctcaa tactggtttt cgccatctct tgcgcccact gtttcatttc actaaacagt
      301 tgctgtgcgg tttgctgttc acccagcagt cgcagcgcca ttccttgcca gaagagataa
      361 tcaaccggct gatcgttgta ataactgtgg atgttaatgg tgcgatcgcc ggtcgccgcc
      421 agacgtaaac aacgcatcgc ttcagtttca tcgccctgcg cgttggcgca tatcgcctgc
      481 cagaaccaga tgtcgttatc agtttgcccc ggtaaacggc cttcgcttaa attctccgga
      541 taatgcagcg cggcatgaag cagttcgctg gcctgctgcg gctctctggc atcaagatgc
      601 tgccaggcgc gtaataactg gttgaggata aactgactgg tgaccttccc ttccccgcct
      661 tcccacgggt ggaatttacg cgtggcgaga atgtccgccg ctttgtctgc ctgacccgtg
      721 agatgccaca aattgagcag ttctgcggtc atgtcgtcgc gtttcagcgc aatttccaga
      781 ttattttcca gacgcgccag tcgtttctcc ggtgtggctc cacttaactt atcaagcaaa
      841 tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct gataagcatt atcaagataa
      901 cgcgcggcca gctcataatc gtgttgctta ttccacgcat ggatcgctaa cccgcgccag
      961 ccgtcggcaa actccggcga catctctacg caacgttgcc aaaaggcaat ggctttgttg
     1021 tagctacgtt tgttgtagta gaagcaggcc agtaaatggc gagcaaacca gcactcttca
     1081 atactctcca gcgccgccac ttcttccagc gtattcggga aacggacaaa ctgcgggaag
     1141 acatcaatgg cttttgcgac cagttcgcca cgttcggctt tcggcagcaa gctggcttgc
     1201 aggtaaagcg gcagcgtgcg ctggcagtcc agtgcgttca gcatctctgc cgccagggtg
     1261 ggcattcccc agttaatcag ttgcccggcg gtcatcaggg cgttaacgtc gcgtccctga
     1321 cacagaccgc gccactgcgc gagagctgat tcgctacgac catcgaacca gttcagccac
     1381 cacagagtgg cgttcagcgg ataatcgcgc agcagtttct cgcgctgcac acgcgcgttg
     1441 tcctgacgac cacttaacac cagcagcaga ttatgcaggc aaagcacttc ctgattggtt
     1501 gggcaggcgc gaagactttg ttggcaaaaa tccagaccag cgtcgaagtt accattacgc
     1561 gccgccagtc gtgccaggcc ataatagcca ccggctttgc tgttgccgct ccagaccgca
     1621 cgccagaaat cctcttcggc ttgttgatat tgtccctgac gttcgtaagc actggcgcga
     1681 atcaaactcg cctgtccgca ctgcggattt ttgttcagcg catgtgcgcg tttcagagcc
     1741 tgagtggcat acgccaccgc ttgcgggaaa tcggcgcggt tatattccag catcgccagc
     1801 gccaggttac agcggtaatc cagcggatcc agcgccacgc cgcgcaggta gtaatcgaac
     1861 ggtgaacggc tggcgtgatg atattgctcc agatgctgac cgataaacca ggcttcatct
     1921 gtactggtaa tgtcttgtgc tgccagtggc gctttggcga cgtccagcaa cggcaactct
     1981 tgcggctgat gttcctgata actcagtaca atattgccgt cggcatcgga gagctcaatg
     2041 gtcagccttt caggattgat accgtgcaac acgccctgga tggcggtcgc aggcatcagt
     2101 gccacggcat catcaagtaa cgcgttgcat ttgccgattt cgcggatcac caggcggtat
     2161 ccgttcaacg gagagatggc atacagcccc cactcaatcc cccgcttact acgctggagt
     2221 tttatcaccg catcgcggga ggcattttgc accatgccca aagaatgata aggcaggaaa
     2281 tactgctcaa aacgcttctc ttcgtaagca tcaagccagg taaaatcggg ctggttatcg
     2341 gcaaaaatac cggtcatcag ttcgatatac gggccgttat tgtcggtcag actcttatcc
     2401 cacgcctggc caaattcact gtgtccccaa ctccactgtt ttttacctgg cgcaatatgg
     2461 tggttggcaa cgtgcagcaa accgccatct tcatcgtgac accacgcgcc gacaaaatcg
     2521 tactgcgatt tttcggccat atatgaggtt gtaacaggca catttttata gcaagaaatg
     2581 tccactccgg cggagtagtc cactttgtag taagtgccgg tggcgatggg gaaagcggag
     2641 acggcccgtt tgccgtgatc aaacaccgca gtcacatccg gtgggaaaac gctctgatgc
     2701 ccttcccccc ctttcactgc cgggttggcc caccacaaga aatgacgcgg cgtggcgtta
     2761 ccgttataga cgcggctggc gatttccagc gccgcccggt cagggcgcag ggtgaaacct
     2821 gtcatcacct gtaaaccatg catcggctcc gtttcgccta cccacaccgt ctgtgcaccg
     2881 tcttcatggg cttcgagggt gaaatcaacg ggcataaagg tggtcgggcg atgatgttgc
     2941 ggccagttaa actcaatccc accggaaatc cacggcccca gcagccccac cagcgcaggt
     3001 ttaatgactt cattgtgata aacaaaatcg cgctgtttca ctttatccca cgcgcgatgc
     3061 acccgaccgc ccagttccgg caggatcatc actttgatgt agtcgttttc cagccacacc
     3121 gcctgccagg atttcagggt tttctgctcg ctcagcgtat cggtcacgcc gtagggataa
     3181 accgcgccgg acgatccctg ataaacgcga ttttccagga acatgggatg tatatcctgc
     3241 ggcccggttt cataggtcgg gatctcaacg cgctcttgcc acacttttac tggagtcatg
     3301 gtgccctcaa tattaacaag acatactgaa ttaaaagatt tgtggcagtg tattgaacaa
     3361 tctggcaatg ttttcgcgga ataatcacgc aattaactaa acaaggttta gtgaagatga
     3421 gagcctgcat taacaatcaa cagattcgcc accataacaa atgcgtgatt ctggaactgc
     3481 tgtaccggca aaagcgcgcc aataaatcaa cgctggcccg gctggcgcaa atttcgattc
     3541 cggcagtcag taatattttg caggaactgg aaagcgaaaa acgggtggtg aatattgacg
     3601 atgaaagcca gacgcgcggg catagtagcg gtacatggct gattgcgccg gaaggtgact
     3661 ggacgctgtg cctgaacgtg acgcccacca gtattgagtg tcaggttgct aatgcttgtt
     3721 taagtccgaa aggtgaattt gagtatttac agattgatgc accgacgccg caggcgctgc
     3781 tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt gtggccggac cgcaccatca
     3841 atctggcgct ggcaatccac ggtcaggttg atccggtgac cggcgtgtcg caaaccatgc
     3901 cgcaagcgcc gtggacaacg ccggttgagg tgaagtatct gctggaagag aagctgggca
     3961 ttcgggtgat ggtcgataat gactgcgtga tgctggcgct ggcggagaaa tggcaaaata
     4021 attcgcagga acgggatttc tgcgtgatca acgttgatta cggcattggc tcgtcgttcg
     4081 tgattaacga gcaaatttat cgcggcagtt tgtatggtag cggacaaatt ggtcacacca
     4141 tcgttaatcc ggatggcgtc gtctgcgact gtggacgtta cggctgcctg gaaaccgtcg
     4201 cctcgttaag tgcattaaaa aaacaggcgc gggtatggct aaaatcacaa ccggttaata
     4261 ctcagcttga tcctgaaaaa ctgacaaccg cgcagttaat cgctgcctgg cagagtggag
     4321 aaccgtggat caccagctgg gttgatcgct ctgccaatgc cattggtttg agtctgtata
     4381 acttcctcaa catcctcaat attaatcaga tttggttgta cggtcgcagt tgtgcctttg
     4441 gtgagaactg gcttaatact attattcgcc agacaggatt taacccgttc gaccgcgacg
     4501 aaggaccgag cgtgaaagcg acgcaaattg gcttcgggca attaagccgc gcacaacagg
     4561 tgctgggaat tggctatttg tatgttgagg cgcagttacg acagatttga tggcgcgata
     4621 acgtagaaag gcttcccgaa ggaagccttt tttcaacaga ttaaccgttt cgcttatgcg
     4681 taaaccgggt aacgtgcgca gatgtcgaga actttacctt tgatgcgctc gataacggct
     4741 tcatcattga tgctgtccag cacgtcacac atccagccag ccagttcttt cgcttctgct
     4801 tctttgaagc cgcgacgcgt aattgccgga gtacccacgc gaataccgga ggtcacaaac
     4861 gggctcttcg gatcgttcgg tacgctgttt ttgttgacgg tgatgttagc acggcccaga
     4921 gcggcgtctg cttctttacc ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg
     4981 ttatcagtgc cgccggaaac cactttgtag ccgcgctcga ggaacacttc taccatcgct
     5041 ttagcgtttt tcgcgacctg ctgctggtaa gttttgaact caggctccat cgcttctttc
     5101 agagcaaccg ctttaccggc gattacgtgc atcaacggac cgccctgacc accagggaaa
     5161 acggcagagt tcagtttttt gtacagctct tcgctaccac ctttcgccag gatcaggccg
     5221 ccgcgcggac ccgccagggt tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc
     5281 gggttcgggt agacgccagc agcaaccagg cccgcaacgt gcgccatatc aacgaacagg
     5341 taagcaccga tgctgtcagc gatttcacgc attttcgccc agtccaccac gccggaatat
     5401 gcagagaaac caccgataat cattttcggc ttgtgttctt tggcttgttt ttccagatcg
     5461 gcgtagtcga tatgaccggt agcatcgata ccgtaaggaa cgatgttgta cagtttaccg
     5521 gagaagttaa ccggagaacc gtgagtcagg tgaccgccat gcgccaggtt catacccaga
     5581 acggtatcac ctggttccag cagcgcggtg tagaccgcaa agttagcctg ggagccggag
     5641 tgcggctgga cgttagcgta gtcagcgccg aacagttctt tcgcacgatc gatcgccagt
     5701 tgttcaacga tatcaacata ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca
     5761 gcatatttgt tggtcagctg agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt
     5821 tcggaggcga tcagttcgat gtgctcttcc tgacgtactt tttcctgctc catagcctgc
     5881 cacagttcgg catcataatc ggcaatgttc atttcacgct ttaacatccg catctcctga
     5941 ctcagctaac aataaaattt ttggccttta taggcggtcc tgttggacaa cggcgaacag
     6001 tataaccgaa tcattgtgcg ataacaggtc ttgacaaagg aatttacgca aacgattacc
     6061 ttcaggctac gcaaggcttt ggagaataaa gagcttgcaa ccggaaacgg atttcttttc
     6121 aggtttgtga tgcaaatttt tcacttcatc acattctttc tgaaaaacac caaagaacca
     6181 tttacattgc agggctattt tttataagat gcatttgaaa tacatcaatt aagatgcaaa
     6241 aaaaggaaga ccatatgctt gacgctcaaa ccatcgctac agtaaaagcc accatccctt
     6301 tactggtgga aacggggcca aagttaaccg cccatttcta cgaccgtatg tttactcata
     6361 acccagaact caaagaaatt tttaacatga gtaaccagcg taatggcgat caacgtgaag
     6421 ccctgtttaa cgctattgcc gcctacgcca gtaatattga aaacctgcct gcgctgctgc
     6481 cagcggtaga aaaaatcgcg cagaagcaca ccagcttcca gatcaaaccg gaacagtaca
     6541 acatcgtcgg tgaacacctg ttggcaacgc tggacgaaat gttcagcccg gggcaggaag
     6601 tgctggacgc gtggggtaaa gcctatggtg tactggctaa tgtatttatc aatcgcgagg
     6661 cggaaatcta taacgaaaac gccagcaaag ccggtggttg ggaaggtact cgcgatttcc
     6721 gcattgtggc taaaacaccg cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg
     6781 acggtggcgc agtggcagaa taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg
     6841 aaggtttccc gcatcaggaa attcgtcagt actctttgac tcgcaaaccg gatggcaaag
     6901 gctatcgtat tgcggtgaaa cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc
     6961 acgccaatgt tggcgatgtc gtgaaactgg tcgctccggc aggtgatttc tttatggctg
     7021 tcgcagatga cacaccagtg acgttaatct cttccggtgt tggtcaaacg ccaatgctgg
     7081 caatgctcga cacgctggca aaagcaggcc acacagcaca agtgaactgg ttccatgcgg
     7141 cagaaaatgg cgatgttcac gcctttgccg atgaagttaa ggaactgggg cagtcactgc
     7201 cgcgctttac cgcgcacacc tggtatcgtc agccgagcga agccgatcgc gctaaaggtc
     7261 agtttgatag cgaaggtctg atggatttga gcaaactgga aggtgcgttc agcgatccga
     7321 caatgcagtt ctatctctgc ggcccggttg gcttcatgca gtttaccgcg aaacagttag
     7381 tggatctggg cgtgaagcag gaaaacattt attacgaatg ctttggcccg cataaggtgc
     7441 tgtaatttga tgttgccgga tggaaacatc cggcaaccct tgacgcggtt taaattgccg
     7501 cgtcgtcctc ttcaccggta cggatgcgaa tgacccgtgc cacgtcaaag acgaagattt
     7561 taccgtcacc gattttgccg gtttgcgccg tgcgaataat ggtatcgaca caggtatcga
     7621 caatgtcgtc cggtacgaca atctcaattt tcactttcgg cagaaaatcc accatatact
     7681 ccgcgccgcg gtacagctcg gtatggcctt tctggcgacc aaagcctttc acttcggtca
     7741 ccgtcatgcc ggtaataccg acttcggcca gtgcttcgcg gacatcgtcc agcttgaagg
     7801 gttttataat cgcatcaatc tttttcatgc tattccttga aaaggtcgcc tgtcttttga
     7861 tctgctaaac gtaacacata acgccaattc attccttgaa atcgtttgca tccagctcgt
     7921 gtcgggaaag cagtttataa aattctgtcc ggttgcgccc cgccattctc gccgcgtggg
     7981 tgacgttgcc tttggtaatt tgcagcagct tacgcaaata gttgagttca aactgattac
     8041 gtgcctcaac aaaggttggc agcgccgtgt tttcaccctc cagcgcctgc tctactaatg
     8101 catcgctaat aaccggagat gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact
     8161 ggcgcacatt acccggccag ctagcggtca tcaggcgttt catcgcatca gtggagaacg
     8221 cgcggacaaa cggtttatgt cgctctgccg cctggcgcaa caggtgattt gccagaagcg
     8281 gaatgtcttc tgtgcgctct gccagagccg gaattttcag gctgacaacg ttgaggcggt
     8341 aatagagatc ttcgcggaat tccccgcgcg ccatcgcttt tggcagatca cggtgagtgg
     8401 cagaaataat ccgcacattg atatcaatat cgcggttact gcccagcggg cgcactttac
     8461 gctcctgcaa cacgcgcagc agtttgacct gtaacggcgc aggcatatcg ccaatttcat
     8521 cgagaaatag cgttccgcct tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc
     8581 cagtaaacgc gccacgcgca tgaccaaaca gctccgactc cagcaattgc tcgggcaatg
     8641 cgccacagtt aatagcaata aatggtttgc tgttgcgcgg gctggcgttg tggatagcct
     8701 gggcgaaaat ctctttcccg gtgccgctct gaccgttaat caaaacgctg acgtctgatt
     8761 gcgccaccag ccgcgcctgt tccagcaaac gcagcatcag cgggctgcgg gtgacaattg
     8821 cctcgcgcca gcgttcatcg gtggctggcg cggattgctc cagcgcatcg tcaattgcct
     8881 gatatagcgc gtctttgtcg acaggcttgg tgaggaaact aaaaacgccc tgctgtgttg
     8941 cagcaacggc atcggggata gaaccatgcg cagtaagaat aattactggc attcccggct
     9001 gcactttctg gatttcagca aatagctgca taccgtccat ttcatccatc cgcaggtcgc
     9061 tgataactaa atctactttt tcgcgattca gtacccgtaa tccttcagcg ccactttccg
     9121 ccgtgaccac actgtagcct tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg
     9181 gatcgtcatc gaccaataat aaatgcgcag gtttatggct catcaggagt gacctcatgg
     9241 gtggatggcg cgggcttttc actctcgtgc ggcgtatccg gcgagaaatt tccagccggt
     9301 ttgcgggtcg agagctggcg ttcaatatcg gtcaggtttt ccagcttgcg ggtggtgagt
     9361 tccagttgct gctgtagaac gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg
     9421 gactgctgga gtttactgta acgttggcgc tcttccgcca gttgcagttg cagcgcctga
     9481 ccgtcgcgcc agagttgata cagtgggcga acctgtgccg ggatctcggt acttaacgct
     9541 tcaatacgcg cgaccagctg gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa
     9601 atcccttgtt taaaggtatt ttgccagcag ccgtcgtcat attggcgagc ttgctgacgc
     9661 gactgcgcag gcattaaacg atcagcacaa tccatcgccc gcagccagta aagcggattg
     9721 gtttcggttg atttaccttg cagcgcccag atgtcgctac attcagtaga aagatagtca
     9781 gccagttgat aaaccggaat tttttcttct gctggcgtat caatggctgg cttattgtga
     9841 ttctgcacgc aacccagcaa tgccagacat ggcagccctg ccagccacag ccgtcggggc
     9901 aataatcgtt gaaaaatgtg tcgcatattc accagactta aagcctatcc cagtgggcgt
     9961 aattgttgca gacagtctgg acatggacag cgcggagaaa ccggagcgta catatcgtac
    10021 gtgaagattt cgagcactgc ccggagccga actgacaaat aaaatagcct gataggatag
    10081 gctgttagca ttatttcgtg tttttcgacg acggtaattc aatgcggaaa caaacgtctt
    10141 gcccgctctc gtcgaccaga tacagttccc cttgcatacg gcgaatacaa tccctggcaa
    10201 tgcttaatcc cagaccgctg cccttcaccg ccccttttcg ctggtggctt ccctgaaaaa
    10261 agggttcgaa gatcatggcg cgttcctctt gcggaatggg cgtgcctgta ttgatgacat
    10321 caatataaac ccgcgcacca tgtgaactgc tgcgaaggca aatgttaccg gattcagccc
    10381 cgtagtgcac cgcattggag taaagattat ccagtacgct catcagcagc attggctccg
    10441 ccaggcaagc tgttgctttg agatcgacgt cggtatgcat cattttagcc cgtgcgggca
    10501 ggctatgagc agaaaccact gtctccacca gcggtgctaa ttcaacattc tccagttcca
    10561 ccgcactgtc cgcctgttta cggttgtaat caagcagttg ttcgatcagt ttttgcaaat
    10621 tgcggctgct gctatcaaga atgctcacca cctctttttg ctctggcgta agcggcccga
    10681 caacctggtc agccagtaat tcagtgccct cgcgcatact cgccagtggc gtttttaatt
    10741 catgagataa atgtcttaaa aattgatggc gttgggattc cagccatgac aggcgctcac
    10801 ttaaccaaag aatacgttgc ccaaccgagc gtaactcgct cggtccactg aacgaaacgc
    10861 tattgcccag agaacgccct tcccccagac ggttgatcat gcgctcgata tttttcaccg
    10921 gcccgataat catccgcgtg aaaagtagca ccatcaccag actcaccaga aatagcacca
    10981 gcgattgcca accaaaatat tgcccacgtt cggcgatttc acgctgaagt tgctgcccac
    11041 gagagaacac cactgtgcgc gtggcctgca ccatttcggt attggcactg gcaaaggctt
    11101 caagacgcgc ggcggcggca gcatcgggac cactgttgtt acactgaagt tgggccagat
    11161 tgttcaaatc ctgacgcaat gcctggtaaa gtttatcgtc tggcagtacg cctgcgtggg
    11221 catcgagcat ttcgctgtaa cgcttgcgct ggctttgata aaccttcgca agcgtagggt
    11281 cgtccagcac gcaatactga cggtagctac gctccatctc cagcgccgcg ttggtcatcg
    11341 cttcactacg ccgggcatca ataagcgtag tgcggttaac cagcgccgcc tgatcgctaa
    11401 gcgcattcag gctttgccag gcttgccatg ccagcaccaa caggggtagt agaattagca
    11461 aaaatgccag cattaccagt tgtcgtaatg agcggggaaa aacgggccag cgtttcaagg
    11521 tgttactctc gtcagacgcg aatagcctga tgctaaccga ggggaagttc agatacaaca
    11581 aagccgggaa ttacccggct ttgttatgga ataaggcggt gcctaactcg acgtttcgcc
    11641 cgatggttga tatagctacg ctgatatcag aagttggacg gcaggcacct tgttgtgcgt
    11701 cattcgaatt ttatgtagca cgtcccgaag gggctgacat aagtcggtga atgagccact
    11761 gcttactatt atgcagtaac tgtgccaata aagaaaatag tttggtaacg tactgattat
    11821 acgttgcttt gagggattta tggcttcttc gctatttgaa tgtaaatcag ccactgtgtc
    11881 gctaaaaaga gacagcttaa gataaactta ttagattaca ataaaatcaa tcacttaagt
    11941 gtcgccagaa agcgacacgg taaaccaccc attgtcgtga tttacagaca caaaaaagct
    12001 cccggagttg ggagcttatg atagtggttg gtgcttaatg ccgcatccgg gctgcaaaac
    12061 caatgaactg atgaattacc ccaactgctt acgcgcattg cggaaaatgc gcatccatgg
    12121 gccatcctcg ccccagtttt ccggatgcca ggagttgctg acagtacgga aaacacgttc
    12181 cgggtgcggc atcataatgg tgactcgacc actttcagtc gtgactgccg taataccgtt
    12241 cggtgaaccg ttcgggttgg ccgggtaggt ttcagtgact ttgccgaagt tatcgacata
    12301 gcgcagtgcc accagccctt tgctttccag tgccgccaga tgcgccgcat cacgcacttc
    12361 cacgcgccct tcaccatgag agacagcaat cggcatttgc gagcccacca tcccctgcaa
    12421 caacagagac gggctttggg ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga
    12481 ggtattgcgc acaaaacgtg gccacaactc actacctggg atcagttcgc gcaggttaga
    12541 catcatctgg caaccgttac atacccccag cgccagcgtt tgcggacggt ggaagaaggt
    12601 tgcaaactca tcgcgtacac ggtcattgaa caggattgac ttcgcccaac cttcaccggc
    12661 acccagcaca tcaccgtagg agaaaccacc gcacgcgacc agggcgtgga aatcttccag
    12721 gcccgtgcgt ccggtcagca ggtcactcat atgcacgtcg atagcatcaa agcctgcacg
    12781 gtggaaagct gccgccattt caacatgcga gttcacgccc tgctcacgca gtacggcaac
    12841 tttcggacgt gcgccagtgg caatatacgg tgctgccaca tcttcgttga tatcgaacga
    12901 cagttttaca ttcaggcccg gatcggcgtc gttagatttc gcctgatgct cctgatcggc
    12961 acactccggg ttgtcacgca ggcgctgcat ctgccaggta gtttctgccc accagacacg
    13021 caacgtggtg cggctttcgc tgaatacagt ctgcccgttg gcggtaatca caaaacggtc
    13081 accggaaacc gcctgcccta cataatggac acaatcagca agcccatgct gtgccagtac
    13141 ggactcgacc gcttcacggt cagcggcacg aacctgaatc accgcaccca gttcttcgtt
    13201 aaacaacgcc gccaggcgat cgtcgcccag agaggcaata tccgcgttaa tgccgcaatg
    13261 accagcaaag gccatttccg ccagcgttac cagcaggccg ccatcagagc ggtcgtggta
    13321 tgcgagcagc ttacgctgtg caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg
    13381 cgcgacgtcg cgtacatctg ccggtttgtc gccaagctga cgataaacct gcgccagcgc
    13441 cgttgcgccc agcgcgttat tgcctttgcc caaatcaatc agcaatagtg cgttatcttc
    13501 ggtagaaagc tgtggcgtga tggtgtgacg cacatcttcc acgcgggcaa atgcagaaat
    13561 caccagcgac agcggcgacg tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt
    13621 catcgacata gagtctttac ccaccgggat cgtcaggccc agcgccggac aaagctcttc
    13681 gcccacggct ttaacggctt catacaggcc cgcatcttca ccagggtggc ctgccgccgc
    13741 catccagttg gcggaaagtt taatgcgttt gatatcgcca atctgtgttg cggcaatatt
    13801 ggttaacgct tcaccgaccg ccagacgggc agaggcggcg aaatccagca gcgcaaccgg
    13861 cgcacgctcg ccaatcgcca tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac
    13921 cgcgcagtta gcgaccggca cctgccacgg acccaccatc tgatcgcgcg ctaccatgcc
    13981 agttacgctg cggtcaccga tggtcaccag gaaagttttc tccgccacgg tcggcagatg
    14041 cagtatgcgt ttcaccgcgt ctgcaatggt gatcccttca cggaccagcg catcgccctt
    14101 cgctttcagc gtttgtacat cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag
    14161 cggcagatcg atcggctgat tatcaaaatg acgatcgtgt agagaaagat gcagttcttc
    14221 ggtcgcttca ccaatcaccg cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag
    14281 cggtaattga tcggcagcaa ccgccagcac gtagcgttcc tgggattcgt tacaccagat
    14341 ttccagcggg ctcatgcctg gctcgtcgct cagaatgtcg cgcagttcaa atttaccgcc
    14401 acgcccgccg tcgctcacca gttccggcat agcgttagaa agaccgcctg cgccaacgtc
    14461 gtggataaac agtattgggt tggcatcacc aagctgccag caacggtcaa tcacttcctg
    14521 gcagcgacgc tccatctccg ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc
    14581 agactgacca gacgccatgg aagacgccgc gccgccgcca agaccgatgt tcattgccgg
    14641 gccgccgaga acgaccagct tcgcgccaac gttgatctcg cctttttgta cgtgatcggc
    14701 gcgaatgttg ccgatcccgc ccgccagcat gatcggtttg tgataaccgc gcagctcttc
    14761 gccgttgtgg ctgttcactt tttcttcata agtacggaag tagccgttca gtgccggacg
    14821 accaaattcg ttgttaaacg ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc
    14881 ggtgacaatg cgctcaggct taccgaaatc ttcttcccac ggctgttcga agccaagaat
    14941 tcgcaggttg gaaacggaga aaccaaccaa acccgctttc ggcttcgcgc cgcgcccggt
    15001 ggcaccttca tcgcggattt caccgccgga accggtcgcc gcgcccggcc acggagaaat
    15061 cgccgtcggg tggttgtgag tttcgacttt catcagaata tgcgccggtt cctgatggaa
    15121 atcatagcgg cccgtttcgt ggtcagcaaa gtagcggccc acttcagaac cttccattac
    15181 ggcggcgtta tctttataag cagagagaac gtgatctggc gtggtttcga aagtattttt
    15241 gatcatcctg aacagcgatt tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa
    15301 aattttgtgg cggcagtgct cagagttcgc ctgagcgaac atatacagct cgatgtcgtt
    15361 cgggttacga ccaagctttg tgaaagcatc ctgcagatag tcaatttcat cttccgccag
    15421 agccaggcca agacgcaggt tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc
    15481 aacgcttgtg accggagtcg gttgatggtg ggcaaacaac tgctctgcat catctaaatc
    15541 aaaaaagaca gtttccatca tgcggtcgtg cagttcagcg gtaacctgct gccattgttc
    15601 attggtcagc gtaccggctt ctatatagta agcaacgccg cgctcaaggc ggtttacctg
    15661 ttgtagcccg cagttatggg caatatcggt cgctttcgaa gaccagggag agatggtgcc
    15721 aggacgcggg gtcaccagca ggagtttgcc ttgcggggcg tggctggcga gtgccggacc
    15781 atatttcagc aggcgttcaa gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc
    15841 agcaaaatgg acatactcgg cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg
    15901 tgccagcagt ttgttgattc ggaatgccga cagtgcaggc gaaccacgca gaatttccat
    15961 cataagtctc tcgtcttcta agctttcggt gtacccaagg ggggggggaa acgggcgtca
    16021 ttataaagaa tctgatgcgc tgacgaaacc gtttgcgtgg aaataaaatc accatcgtga
    16081 attagcaacg cgtgccgcca atggctgtaa taagttgcca tctggcgcag gtttacgcaa
    16141 aatgccgctc atttatgagt aaacctttca ctattattac gttttttcaa gctgggacgc
    16201 gcacgacaca gagaattaac taattgaaaa aattaaagat taattatctg ttcatcggca
    16261 ttctggcact gctgctcgcg gtcgctctct ggccatccat tccctggttt ggtaaagccg
    16321 acaaccgtat cgccgccatt caagcgcggg gagagttgcg tgtgagcacc attcatactc
    16381 ccctgactta taacgaaatc aacgggaaac cttttggcct ggattacgaa ctggcgaaac
    16441 agtttgccga ttacctcggc gtaaaactga aagtgaccgt gcggcagaat atcagccagc
    16501 tgtttgacga ccttgataat ggtaacgccg acctgctggc ggcaggactg gtctataaca
    16561 gtgagcgggt aaaaaattat cagcctggcc ctacctatta ttccgtgtca caacaactgg
    16621 tttataaagt gggtcagtat cgcccacgta cgctgtgcaa cctgacggcg gagcaactca
    16681 ccgttgcacc gggtcatgtg gtggttaacg atctccagac cctgaaagaa acaaaattcc
    16741 cggaattaag ctggaaggta gacgacaaaa aaggctctgc ggaattaatg gaagatgtca
    16801 tcgaaggaaa actcgattac accattgctg attctgtcgc catcagcctg tttcagcgcg
    16861 ttcacccgga gctcgccgta gcgctcgata tcaccgatga acaaccggtg acttggttta
    16921 gcccgttaga tggcgataat accctttccg ccgccctgct cgacttcttc aacgaaatga
    16981 atgaagacgg tacgctggca cgcattgaag agaaatacct ggggcatggc gatgattttg
    17041 attacgtcga tacgcgcaca tttttacgcg ccgtcgatgc ggtactgccg cagttaaagc
    17101 ccctgtttga gaaatacgcc gaagaaattg actggcgttt gctggccgct attgcttatc
    17161 aggaatcgca ctgggatgca caggccactt caccgacggg tgtgcgcggc atgatgatgt
    17221 taaccaaaaa taccgcgcaa agcctcggca ttacggatcg taccgatgcc gaacagagca
    17281 tcagcggtgg cgtgcgttat ttgcaggata tgatgagtaa agtgccggaa agtgtgccgg
    17341 agaacgagcg gatctggttt gccctcgctg cgtacaatat gggctatgcg catatgctgg
    17401 atgcccgcgc cctgacggca aaaaccaaag ggaatcctga cagttgggct gacgtaaaac
    17461 agcgtctgcc tttacttagc cagaaaccct attacagcaa gctgacttac ggctatgctc
    17521 gtggacatga agcctacgct tatgtcgaaa atattcgtaa gtatcaaatt agcctggtgg
    17581 gttatctgca agagaaagag aagcaggcta cagaagcggc gatgcaactg gcgcaggatt
    17641 atccggcggt atcgcctacg gagttgggca aagagaaatt tccttttctc tcgtttcttt
    17701 cccagtcgtc atcaaactat ttgacccatt ctccctctct gctgttttcc aggaaaggga
    17761 gtgaagagaa acaaaattaa tccgtcgagg attgcgcttt tttctgcgct ttaatttcct
    17821 ggcggcgcat gcgaaagaag tcactgagca acgccgcgca ctcatccgcc agtattcctt
    17881 ccgtaatttc cactcggtga ttcatacccg gatgatgcag cacatccatt aaagatcccg
    17941 cagcgccagt tttcgcgtca cgcgcaccaa agaccacgcg accaatgcga ctgtggatca
    18001 tcgctccggc acacattaca catggttcaa gcgtgacata caacgtggcg tcgatcagac
    18061 gataattttg catcaccaga ccaccctgcc gcagggccat gatttctgca tgtgcggtgg
    18121 gatcatggcg accaatcggg cggttccaac cttcgccgat tacccgattg ttatgcacta
    18181 ataccgcgcc gaccggcact tcccgctcat cccaggcacg tttcgccagc gtcagcgcgt
    18241 gacgcatcca gtattcgtgg ctaaattcga cttcagacaa aaagaaaact ccggttataa
    18301 aagcgcggcg cattatacac ggacgctatg ctttactcta ttccagttgc tggagttcac
    18361 cgcgcggggt tactcgccaa cgatgctgac agaaataaag cagcggattg tcctgattac
    18421 tgtcgctata gccactgtac agccgcagcg gagtgccgat tttgcgctcc agttgtgcga
    18481 ccttttcatg tcccagacaa cgcatcgtca atacccaacc accatagcca cgctgaattt
    18541 ggctggcgat aagattaacc cgcggcagcc agggcttatc gaaataaacc gcttcaacca
    18601 gcggctgcgg agagccggta atcaaccaga tatcagcatc ggaacttaac aggtaggtgg
    18661 ttaatcgctc ctgaaccagc ggaaaggcgg taacattgtc gcgaaaccag cgcacgaaat
    18721 cggcctgcaa cgtctgtaaa cgtgcttcgc tgtgaccaaa agtgcacccc cacagaagca
    18781 gactcatcgg ccagcgtgcc gcacgacctt ttatcaataa cgcaatggct ataatcggta
    18841 acaacggcag gacaagtaac gcattcagcg gttggcgacg tagtaaatag cgcagaaaac
    18901 tgccgaacat atcctgctga tgcaatgttc catctaagtc aaaaaacacc acacgacgct
    18961 cgtgagttgc caaaccatac tcctctggac tgaaacatcc tgattaattt tcgctactac
    19021 atagcctaac agatagatca tcacttttcc ggcaacaatc gaccgcatca tggctaactg
    19081 gaatttttaa ttcatggcaa ttagcggcaa tggaatataa aattcactcg cgtgtgtctc
    19141 atattgcctg ttgtcgccac tatttcatcg cgaggaaaga tatgaacggc ttacttcgta
    19201 tccgtcagcg ttaccagggg cttgcccaga gtgataaaaa actggcggat tatctgctgc
    19261 tacaacctga tacggcgcgc catttaagct ctcagcaact ggccaacgaa gccggagtca
    19321 gtcagtccag cgtcgtgaag ttcgcgcaaa aactcggcta taaaggtttt ccggcgctta
    19381 agttggcgtt gagtgaagcg ctggcaagcc agccggaatc gccctccgtg cccattcata
    19441 accaaatccg cggtgatgat ccgttacggc tggtcggcga aaaactgatt aaagaaaata
    19501 ctgccgctat gtacgcaacg ctaaacgtta atagtgaaga gaaactgcat gaatgcgtaa
    19561 caatgttgcg ctctgcgcgg cggataattc tgaccggtat tggcgcttcg ggtctggtgg
    19621 cgcaaaactt tgcctggaag ctgatgaaga ttggcttcaa tgctgccgca gtgcgcgata
    19681 tgcatgcgct actcgcaaca gtacaggcgt cgtcccctga cgatctgtta ttagccattt
    19741 cctacaccgg tgtacgacgc gagttaaacc tggcggcaga tgagatgctg cgagtgggcg
    19801 gaaaagtgct ggcgattacc ggctttactc cgaatgccct gcaacagcgt gcttctcatt
    19861 gcctgtatac cattgccgaa gaacaagcga caaacagtgc ttcaatctct gcttgtcacg
    19921 ctcagggaat gttaacggat ttgctgttca ttgcgctgat tcagcaggat ctggaactgg
    19981 caccagaacg tattcgtcat agtgaagcgc tggtgaaaaa actggtctga gtaaagaatg
    20041 cgcgtataat gcccgcccgg tttgtgttgt tttgagagtt tccttatggc gttgttaatc
    20101 actaaaaaat gcatcaattg tgatatgtgt gaacccgaat gcccgaatga ggcgatttca
    20161 atgggagatc atatctacga gattaacagc gataagtgta ccgaatgcgt agggcactac
    20221 gagacaccaa cctgccagaa ggtgtgcccg atccccaata ctattgtgaa agatccggcg
    20281 catgtcgaga cagaagaaca gttgtgggat aaatttgtgc tgatgcacca cgcggataaa
    20341 atttaatatt ctactctgga agtagagtat taattatatt actgggaagc cttaacgcca
    20401 ttatatttat ttaattgatg acattagcat aatcattcac taagttaatt tatatagtat
    20461 ctgccaagac acttatttat agttattaaa ggcgcgtccg attggttcac cggacgcacc
    20521 ttaagtacgt ttccttgtgt tataagaaca gaaggatcag ctgtaaaaca gcaatgatga
    20581 ttttgatgac ccgtttaatc aggtatcggc aatcagtcat tcgtttttcc ttaaacaagg
    20641 aatgcagccg ttcagattag cccttacatc tccccaaaac tgaacgtgcg agttattgag
    20701 ggtgcatgct gcactccaca ccagagcttt gacgacacca ctcgtttcaa tgggggaatt
    20761 ctgtggcatg gtgtaaagca cagcaaaatc ttcaataacg aagccaattt taatgtactt
    20821 acgaattggc ggtcacgtta atctttccat caatattgct ttcttcgtaa aggctcgagt
    20881 ttttatgcta aagattgcaa gttgcttgta aaagataagt acactgatcc ataatcgctg
    20941 ttgttgaggg tgcatgctgc acaaaattaa agttaaaaag taaaaccccc gttccttacc
    21001 agttcggggg ttttactttt taaagagaac ggtattattt taactttcaa taattaccgt
    21061 ggcacaagca tagtggcgtt catctgccag cgttacatgc atatttacaa cgccgagctt
    21121 ttccgccagt tttaatgcct caccccatag ccgtagccgt ggtttgccga gctcatcatt
    21181 gaatacttca aattgattaa acgccagacc attgcggatc ccggtgccaa acgcttttgc
    21241 tgcggcttct ttcacagcaa aacgcttcgc cagaaaacgc accggctggt ggtgcgtttt
    21301 ccagattgcc cattcgttat cgcttaatac gcggcgtgct aggcgctcac cggatcgggc
    21361 gatcactgct tctatgcgag cgatctctac gatatccgtg cctaaaccta atattgccat
    21421 tagccacgcg cttccagcat cagacgcttc atttctgcca ccgcatcttt cagtccggtc
    21481 atcactgcac ggccaataat ggcatgaccg atattcagtt catgcatctc agggatggcg
    21541 gcaatggctt tcacgttgtg ataggtcaga ccgtgtccgg cgttaacttt cagaccgaga
    21601 ctagcggcaa aggtcgcggc tttggcgata cgcaccagct cttgcgcctg ttcggcgtca
    21661 gttttggcat cagcatagca accggtgtgg atctcgataa acggtgcgcc aacctctgcc
    21721 gcagctttga tctgctcttc atcggcgtcg ataaagagag aaacctgaat cccggcatct
    21781 gtcagacgtt tgcaggcatc gcgcattttt tcacgctgcc ctgcaacgtc caggccgcct
    21841 tcggttgtta cttcctgacg cttttccggt accaggcagc aaaaatgtgg cttcgtctca
    21901 acggcgatcg ccagcatctc ttcggtcact gccatctcca gattcattcg ggtatccagc
    21961 gtctgccgca ggatgcgcac atcgcggtca gtaatgtggc ggcgatcttc acgtaaatgc
    22021 acggtaatgc cgtccgctcc cgcctgctcg gcaataaacg ccgcctgcac cggatccggg
    22081 taagcagtac cgcgtgcgtt acgcagcgtt gcaatatggt caatgttgac gcctaacagt
    22141 aattcagcca tgacaatcct catcattcat aatgtgtttt caccgttcgc ttaggcataa
    22201 actgccggaa cagttccctg ctctttaatg gtttaccgcc aagatacggc ttaagcgcca
    22261 tgcgggtaaa gcgtttcgcg gcgcgtagtg tgtctgcgtc aggaaattcc cgcgcgttta
    22321 acgcttttaa ctgccttccg gtgaacgttt tattgtcgat aacgacgctt gcgataaacc
    22381 ctttttcttc gcgataacga tacgtcatgg tgtcatctac cggctcgccg ctacccgcgc
    22441 aataggtaaa attgacgcca tagcccagat gcccgagcaa tgccagttca aagcggcgca
    22501 gcgcgggttc tggcgtacca gtggcacctg caagagactg aatgcagtgc aaataatcga
    22561 aaaagagttc agagaagcgc gtctcgtatt ccagtacgcg ggagagaagt tcgttgatgt
    22621 acagaccgct gtaaagcgtg ataccgctta atggcagcgc cagcgagaca gcttcagcac
    22681 tgcgcagcgt tttgacttcg ccacgcccgc cgaaacgtag caagagaggg gtgaaaggct
    22741 gtaatgcacc tttcagagta gagcgtttag agcgtgcgcc tttggcaacc agacgcacgc
    22801 gacccgattc ctccgtgaag acgtccagca tcaggctggt ttcgctccac gggcgactat
    22861 gcaggacaaa tgcgcgctgc cagccttcca tcggagtgac tcttaaagat cgtcaacgta
    22921 accgagactg cgcagtgcgc gttcgtcgtc ggcccaaccg gatttcactt ttacccacag
    22981 ttcaaggtgg acaggcgctt cgaacatttc ctgcatgtct ttacgcgctt caatcccgat
    23041 ggttttgatc ttggcccctt tgttgccaat aaccatcttc ttctgccctt cacgctcaac
    23101 gagaatcaaa ccattgatgt cataaccacc gcgttcgtta gagacgaaac gttcgatctc
    23161 cacggtcacg gagtacggca gttcagcgcc gaggaaacgc atcagttttt cgcggatgat
    23221 ttcagacgcc ataaaacgct gtgagcgatc ggtgatgtaa tcttccggga agtgatgggt
    23281 cgcttcaggc agatgcttac gcacgattgc tgcgatagtg tcgacattca gcccggtttc
    23341 agcagagatc ggcacgatat cgaggaagtt catctgactt gccaggaact gtaagtgcgg
    23401 cagcagatcg gctttttcct gcacgttatc cactttgttc accgcgagga tcaccggcgc
    23461 tttgccatcg cgcagtttgt tgagcaccat ttcgtcgtcc ggcgtccagc gggtgccttc
    23521 aacgacaaaa atcaccagct caacgtcgcc aatagagctg ctcgccgctt tgttcatcag
    23581 gcggttaata gcgcgttttt cttccatatg caggccaggg gtatcaacgt agatcgcctg
    23641 atacgcgcct tcagtatgga tccccacaat gcggtgacga gttgtttgcg ctttgcggga
    23701 ggtgatggag atcttctgcc ccagcagttt gttcaacaat gtggatttgc caacgttcgg
    23761 acgtccgacg atggcaataa atccgcagta acttttatcg atgctcattc cagctccagt
    23821 tttttcaacg cctgttcggc ggcagcctgc tcagccttac gacggcttga acctgtgcca
    23881 accaccggtt cactcaggcc gctgacctgg cagtggatag taaattcctg atcgtgcgct
    23941 tcgccacgta cctggactac cagataagtc ggcagcggca gatggcgacc ctgcaaatat
    24001 tcttgcaagc gcgttttcgg atctttttgt ttatcgcctg ggctaatttc gtccaaacga
    24061 gtttgatacc agttgaggat taatttctcg acggtttgaa tatcactgtc gaggaatacg
    24121 ccaccaatta atgcttcgac ggtatcggcg agaattgact cacgacgaaa tccaccgctt
    24181 ttaagttcac caggccctaa acgtaagcac tcgcctaact caaattcgcg cgccagttcc
    24241 gccagcgtat tgccacggac cagcgtggcg cgcatccggc tcatatcgcc ttcatccaca
    24301 cgagggaaac ggtgataaag cgcattggcg ataacgtagc tcagaataga gtcgcctaaa
    24361 aattctaaac gctcgttatg tttgctgctg gcactacgat gagttaatgc ctgctgcaac
    24421 agttcctgat gattaaaagt gtagcccagc ttccgttgaa gccgattaat tacgatgggg
    24481 ttcatgcgat accaataaat aaatgcgtca acaattcagc acacgaaaca gacctgatat
    24541 acatggctct gctaactgct tcgctgcagt ttcagtttac cggtatatgg ggaccaacgc
    24601 tgtttcgtgt gccgtggcaa cctggaggtg ccaaccttaa acttcggggg aatattctat
    24661 acacaacgac gggggatgtc gttagccacg ggagatttat ctcataaata attcacgttg
    24721 tcgccataac ggcgacaacg tgaacgaaga tgggctatta atggatgccg ccaatgcgac
    24781 ttaagcgcac acctgtcggc cattcgcctt cttgcttatc gaaactcatc cagatagccg
    24841 ttgcccgacc gaccagattc gcttcaggca caaaacccca gtaacggctg tccgcgctgt
    24901 tgtcgcggtt gtcgcccatc atgaagtatt gtcccggcgg aacaatccag gttgccagtt
    24961 gttgccctgg ctgctggtaa tacatcccca cctggtcctg cgcaatcggc actgtcagaa
    25021 ttcggtgcgt cacatcaccc agtgtctctt tacgctcgga aagacgaatt ccattttctt
    25081 tggtttcgtt tttcggcact tcaaagaatc cgctggtcgc ttccccacca ttacggcgtg
    25141 agaaggtctg aacgaaatcg ctcggttcca cgttggagta ggtgaccggt agcgcgtttt
    25201 cacacgcctg gccggaactg catcccggtt gaatcgtcag ctcttttgag accggatcgt
    25261 aagtgacttt atcgcccggt aaacccaccg cgcgcttgat gtaatcaagc tttggatctt
    25321 ccggatattt aaagaccacg atatcgccgc gtttcggatg accggtttcg atcagcgttt
    25381 tctggtagat aggatcttta atgccataag caaacttctc taccagaata aaatcaccga
    25441 ttaacagagt cggcatcatc gaacctgacg ggatctggaa cggttcataa ataaacgaac
    25501 gtacaatcaa tacgatagcc agcaccggaa aaacagaagc accggtttcc agccagccag
    25561 gcttcggcgc aacctttttc agggttgctt tatccagtga gtcaccggca gccgcctgcg
    25621 ccgctgcctg acgttcccgc cgtttaggtg cgaaaaagaa tttatccacg caccataaaa
    25681 tgcccgtcac cagtgtggca atcaccagaa tcagggcaaa catattcgcc atgccaactc
    25741 ctaagggtta tttgttgtct ttgccgacgt gcagaatggc gaggaacgct tcctgcggca
    25801 gctcgacgtt accgatctgc ttcatgcgtt tcttaccttc tttctgcttc tgcagcagct
    25861 ttttcttacg gctgatatcg ccgccataac atttagccag tacgttttta cgcagctgtt
    25921 tcacggtgga tcgcgcaata atgtgcgtac caatcgctgc ctgaatggcg atatcaaact
    25981 gctggcgtgg gatcagatct ttcatcttct ccaccaactc gcgaccgcgg ttttgcgaat
    26041 tatcacggtg ggtgatcagc gccaacgcat caacacgttc accgttgatt aatacgtcta
    26101 cacgtaccat gtcggacgcc tggaagcgtt tgaagttgta atccagagac gcataaccac
    26161 gcgaggtaga tttcaggcga tcgaagaagt cgagcaccac ttccgccatc gggatctcgt
    26221 acgtcagcgc cacctgatta ccgtggtaaa ccatattggt ctgcacgccg cgtttttcta
    26281 cgcacagcgt aataacgttg ccgagatatg cctgcggcag cagcatgtga cactctgcaa
    26341 tcggctcgcg cagttcgtaa atgttattta ccgcaggcag cttggatggg ctgtcgacgt
    26401 agataacttc ccttgacgtg gtttcaactt catacactac ggtcggcgca gtggtgatca
    26461 gatccagatc gtattcacgt tccagacgtt cctggatgat ctccatgtgc agcaggccaa
    26521 ggaagccgca gcggaaacca aagcccagcg cgctggagct ttccggctca tagaacagtg
    26581 aggcatcatt caggctgagt ttacccagcg cgtcacggaa ggcttcatag tcgtcggaac
    26641 ttaccgggaa cagaccggcg tatacctgcg gtttgacttt cttaaagcca ggcagcgcct
    26701 tttctgccgg attacgcgcc agcgttaagg tatcgccgac tggagcgccg tggatatctt
    26761 taatcgcaca taccagccag cctacttcgc cacatttcag ttcagtgcgg tcaacctgtt
    26821 tcggcgtgaa gatgcccaga cggtcggcgt tataggtctg cccggtactc atgactttca
    26881 ctttgtcgcc cttacgcagg gtgccgtttt taatacggat aagtgaaaca acgcccaggt
    26941 agttgtcgaa ccatgagtcg ataattagtg cctgcaacgg gccttccgga tcgccttccg
    27001 gcggcggaat gtcgcgcacc agacgttcga gaacgtcctg cacgccaacg ccggttttcg
    27061 ctgaacagcg caccgcgtca gtggcgtcga tgccgacgat atcttcaatt tcttccgcca
    27121 cgcgttcagg atcggctgcc ggcaggtcaa tcttgttcag tactggcacc acttcgagat
    27181 ccatttccat ggcggtgtag cagtttgcca gggtttgcgc ttccacgccc tgcccggcgt
    27241 cgaccaccag caatgcacct tcacaggcag ccagcgaacg ggaaacttca taggagaagt
    27301 ctacgtggcc cggggtgtcg ataaagttaa gctgataggt ttcgccgtca gacgctttgt
    27361 agtccagcgt cacgctttgc gctttgatgg taatgccacg ctcacgctca agatccatgg
    27421 aatcgagaac ctgcgcctcc atttcacggt cagacaggcc accgcagatc tggataatac
    27481 ggtcagacag cgtcgattta ccgtggtcaa tgtgagctat gatcgaaaag ttacgtatat
    27541 tcttcataaa gtatgattat tgtgccttaa tgcccggtta accaggcttt tagaagtcgc
    27601 tgttctgagc ttaacgtctg tattaataga aacgccgcat tctacactac aacattgagg
    27661 cgaggaaatg ttcataccgt atggattgtg gtatctggaa acgtcctcgc atttgttatg
    27721 caaaatgcaa caaagccagt gaaatcactg gctcgcgtct tccgaagatg tttcaaatcg
    27781 cacaaggcca ggcggcaatg ccacgcttaa gatgatcggt tgccattctg cccgggcagc
    27841 aaacttacgc gagtagccgc gcgcaatcag gaacccgcca ataccgccga ggaccgcacc
    27901 acataatgct gcgacgtcag aagcaaagag tagctgaaat agcgaagcga tgaggaataa
    27961 tcccaccagc ggcgacatat aaaccagtaa tgcggagcta agcaggctgc cttcggcgat
    28021 ccccaattcc actttttgcc ccggcactaa cggttcatca cagggtacga caatggtatg
    28081 cgtggtttgc gggccaagtt tattcaacac gcggctaccg caaccggcgc gtgaagcgca
    28141 gctgctgcat gaagctttaa catcacaact gaccagcgcc tgcccgtttt gccaggagac
    28201 gacggtagcc cactctttga tcattgcgct gccccgaact taatattctc ggcaatgcgt
    28261 ttcgccgttt gcggcggcag ttcaccgaca atggtgattt cggcgttatc acgtacgctt
    28321 gtactgacgg ttctgcgtcc ggtgcgcaac atctgatcgg tgctcgatgg cgtagcgcgg
    28381 ttaacgttta ccgagaagct gaataatccg tcagaataga gacgtgattc gataggcatg
    28441 ttgtccatcg tcggtagcgg acgtcgacta ctggaaactt cgctaaaacc ctgtggcaac
    28501 caggttggcg tccagctgaa tttagctttt tcacctacag gaacagaaag caacggcggc
    28561 aaatttgcct tcgccagcgt ctgcatactg ctgctgatat cctgattgac gttaaaagca
    28621 atcacgcgaa attgttccag cgtttcacca tcgcgatcaa ggagatcaac ccgcatcggt
    28681 aatttcgatt cggtgtccat ccacacgatg tagctgtagc gtgtaccatc tcgggcaacc
    28741 acgcgaatga cttcgcaaag acgatcagca atacgcgtgc gcccgacgga gataaagtcg
    28801 tagtaaggag aaaggcgttt gaaatcggta tagatgagcg atggcagaga atcaacaatg
    28861 taatcgccat taagcgtgaa cggttcaagt cccggttcaa aatagctgat ttcatcgccg
    28921 cgctgtacca cttcccggcg cgggccatcc atttgcaaca attgtgcaag aggacggtta
    28981 tcgaggcgtg catgtcgata acgcagagac tcaacaccct gtttattgat gctgatgaat
    29041 gacagctcgt aattcagtga ctgactggcc aggttcatct gctgtaataa cgccccggac
    29101 gcgggagtgg ccgaggcgtt agcagagaat aacaggctac ctgtcactaa tgacatggca
    29161 aaccaaagtt gcttcattac tgcgattgcg ttcctaaagt ttgaattcct ggcacctgta
    29221 cagcggcttg ctgggtttgc gcctgctcaa actgaagctg ttcagagtgg agtcggcgtt
    29281 gcagttcgta atcctgcaac attgcattaa tgcgacgacg ctgctcctgt acctgctgct
    29341 gttgaccatt gtttgcggtc gcttcagaag gtactcccag gcttaccggg ctggctttac
    29401 ccatcatcgg cagtgtatta aataccggcg tttcgggctg ctgggacgtt tcagattgtc
    29461 cattatagtg ctggacgcca acgataactg caagcgatac gcatgctgct acgcccattt
    29521 gggtaagctg tgccgcccac ggacgtactt tctgccagaa tggcattttc tgccattgat
    29581 gcggcgcagg ctgggcttcc gggatcaatg tcgccggttg acgtactggc tcttcttcaa
    29641 tggcggccat cacgcgtgaa gagatatcga aatggagcac ctcgggagta tcaccccgca
    29701 ttgagtcacg gattaagtga tagctttccc aggttttctg catttctggg ttatgagcca
    29761 gttcgttaag cagctcacta tccagcgttt cgccatccat taaagcggaa agttgttctt
    29821 tctgcatgcc taataccctt atccagtatc ccgctatcgt caacgcctga taagcggttg
    29881 aactttgtta tcaatagctt ccctcgctcg gaagatacgt gaacgcaccg tacctaccgg
    29941 acaatccatg atagcggcta tctcttcata gctcaggcca tccagctccc gcaaggttat
    30001 tgccatgcgc aaatcttccg ggagggactc aatagttcgg aaaactatct gtctcagttc
    30061 ttctgacaac attaagttct cagggttcga aatttctttc aacgcgccgc cactttcgaa
    30121 gttttcagct tcaatggcat ccacatcact ggaaggtgga cgacgcccct gagcaaccag
    30181 gtaatttttc gctgtattta cagcaatccg atacagccat gtataaaaag cgctatctcc
    30241 ccggaacgaa tccagcgcac gataggcttt aataaaagct tcttgtacca catcgggaac
    30301 atcacccgac ggcacatagc gggaaaccag actcgccact ttatgctgat agcgcactac
    30361 cagtaagtta aaggctttct gatctccctt ctggacccgt tcaaccagga cctggtccgt
    30421 taactgctcg ctcatccgag gtaaagtctc cccaaaccaa atttccacgc gctatcgaaa
    30481 cgccactcca ttagctgcaa tttgagcaag caaagggtta gagtgtctcg tttttgtaaa
    30541 gttccgtaac gcatctgttt ttgtttgtca tgctgtagac ggatcattat ctatcattat
    30601 aagtctacag aatctgaaca tcgcattatc tgtgtagaaa tgcccattta actgcctgaa
    30661 gagtaaccca acggcctttt tatttcacca cctaatcctc caccagccag taacttctct
    30721 ttttctcgcc gccctgcatc agcgtgttta gcaactgtaa caaatattaa aatagcaggt
    30781 gtttattcgc acaacatgat gctatactga ccaaaccgtg tttagtaaat taaacaaaga
    30841 aaatgaatac tctccctgaa cattcatgtg acgtgttgat tattggtagc ggcgcagccg
    30901 gactttcact ggcgctacgc ctggctgacc agcatcaggt catcgttcta agtaaaggcc
    30961 cggtaacgga aggttcaaca ttttatgccc agggcggtat tgccgccgtg tttgatgaaa
    31021 ctgacagcat tgactcgcat gtggaagaca cattgattgc cggggctggt atttgcgatc
    31081 gccatgcagt tgaatttgtc gccagcaatg ctcgttcctg tgtgcaatgg ttaattgacc
    31141 agggggtttt gtttgatacc cacgttcaac cgaatggcga agaaagctac catctgaccc
    31201 gtgaaggagg acatagtcac cgtcgtattc ttcatgccgc cgacgccacc ggtagagaag
    31261 tacaatccac gctggtgagc aaggcgcaga accatccgaa tattcgtgtg ctggagcgca
    31321 gcaacgcagt tgatctgatt gtctctgaca aaattggcct gccgggcacg cgacg
//
LOCUS       DAJTSL010000033        29261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTSL010000033 DAJTSL010000000
VERSION     DAJTSL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.033"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..156
                     /locus_tag="OO433_001330"
     CDS             <1..156
                     /locus_tag="OO433_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HCR6049872.1"
                     /translation="INYYGGRSETELTTAQLTLITDEGSVNEKQETFIVPMRNAGELT
                     LVKSFDW"
     gene            431..3280
                     /gene="rcsC"
                     /locus_tag="OO433_001331"
     CDS             431..3280
                     /gene="rcsC"
                     /locus_tag="OO433_001331"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6049873.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGGEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGEPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3480..4130)
                     /gene="rcsB"
                     /locus_tag="OO433_001332"
     CDS             complement(3480..4130)
                     /gene="rcsB"
                     /locus_tag="OO433_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6049874.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(4147..6819)
                     /gene="rcsD"
                     /locus_tag="OO433_001333"
     CDS             complement(4147..6819)
                     /gene="rcsD"
                     /locus_tag="OO433_001333"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6049875.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVNTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTISDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQPQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7558..8688
                     /gene="ompC"
                     /locus_tag="OO433_001334"
     CDS             7558..8688
                     /gene="ompC"
                     /locus_tag="OO433_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6049876.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINSRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8800..9855
                     /gene="apbE"
                     /locus_tag="OO433_001335"
     CDS             8800..9855
                     /gene="apbE"
                     /locus_tag="OO433_001335"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6049877.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDEMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GINGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            9929..10981
                     /gene="ada"
                     /locus_tag="OO433_001336"
     CDS             9929..10981
                     /gene="ada"
                     /locus_tag="OO433_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6049878.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCPVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVLANAIGKPKAVRAVASACAANKLAIVIPCHRVVGGDGTLSGYRWGV
                     SRKAQLLRREAENEER"
     gene            10981..11631
                     /gene="alkB"
                     /locus_tag="OO433_001337"
     CDS             10981..11631
                     /gene="alkB"
                     /locus_tag="OO433_001337"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6049879.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTIHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFYPLTTDCRYNLTFRQAGKKE"
     gene            11707..13350
                     /gene="yojI"
                     /locus_tag="OO433_001338"
     CDS             11707..13350
                     /gene="yojI"
                     /locus_tag="OO433_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6049880.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIEMA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLMAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            13568..15214
                     /gene="mqo"
                     /locus_tag="OO433_001339"
     CDS             13568..15214
                     /gene="mqo"
                     /locus_tag="OO433_001339"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR6049881.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMLERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(15364..15852)
                     /gene="eco"
                     /locus_tag="OO433_001340"
     CDS             complement(15364..15852)
                     /gene="eco"
                     /locus_tag="OO433_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6049882.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            16260..16754
                     /gene="napF"
                     /locus_tag="OO433_001341"
     CDS             16260..16754
                     /gene="napF"
                     /locus_tag="OO433_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6049883.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            16744..17007
                     /gene="napD"
                     /locus_tag="OO433_001342"
     CDS             16744..17007
                     /gene="napD"
                     /locus_tag="OO433_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6049884.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            17004..19490
                     /gene="napA"
                     /locus_tag="OO433_001343"
     CDS             17004..19490
                     /gene="napA"
                     /locus_tag="OO433_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6049885.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTLITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTQDATDPLSKETDFKKCAVKLEKV"
     gene            19497..20192
                     /gene="napG"
                     /locus_tag="OO433_001344"
     CDS             19497..20192
                     /gene="napG"
                     /locus_tag="OO433_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6049886.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHRDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            20179..21042
                     /gene="napH"
                     /locus_tag="OO433_001345"
     CDS             20179..21042
                     /gene="napH"
                     /locus_tag="OO433_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6049887.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPRVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            21039..21488
                     /gene="napB"
                     /locus_tag="OO433_001346"
     CDS             21039..21488
                     /gene="napB"
                     /locus_tag="OO433_001346"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6049888.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            21498..22100
                     /gene="napC"
                     /locus_tag="OO433_001347"
     CDS             21498..22100
                     /gene="napC"
                     /locus_tag="OO433_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6049889.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            22119..22736
                     /gene="ccmA"
                     /locus_tag="OO433_001348"
     CDS             22119..22736
                     /gene="ccmA"
                     /locus_tag="OO433_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6049890.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            22733..23395
                     /gene="ccmB"
                     /locus_tag="OO433_001349"
     CDS             22733..23395
                     /gene="ccmB"
                     /locus_tag="OO433_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6049891.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            23437..24174
                     /gene="ccmC"
                     /locus_tag="OO433_001350"
     CDS             23437..24174
                     /gene="ccmC"
                     /locus_tag="OO433_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6049892.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            24171..24380
                     /gene="ccmD"
                     /locus_tag="OO433_001351"
     CDS             24171..24380
                     /gene="ccmD"
                     /locus_tag="OO433_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6049893.1"
                     /translation="MTLAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILCGVEQQRAREARLRAAQQQEAA"
     gene            24377..24856
                     /gene="ccmE"
                     /locus_tag="OO433_001352"
     CDS             24377..24856
                     /gene="ccmE"
                     /locus_tag="OO433_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6049894.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            24853..26802
                     /gene="ccmF"
                     /locus_tag="OO433_001353"
     CDS             24853..26802
                     /gene="ccmF"
                     /locus_tag="OO433_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6049895.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     MLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIAIASLLSGRLDSTYARFTRPWTLAAW
                     IFLTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKA
                     WTLLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAA
                     RGHKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPF
                     FNTMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKV
                     VAMTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQN
                     YSVERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEK
                     RYYNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMAL
                     GGLLCLFDPRYRKRVSPQKTAPEAV"
     gene            26799..27356
                     /gene="dsbE"
                     /locus_tag="OO433_001354"
     CDS             26799..27356
                     /gene="dsbE"
                     /locus_tag="OO433_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6049896.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            27353..28405
                     /gene="ccmH"
                     /locus_tag="OO433_001355"
     CDS             27353..28405
                     /gene="ccmH"
                     /locus_tag="OO433_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6049897.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLSANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(28440..29087)
                     /gene="narP"
                     /locus_tag="OO433_001356"
     CDS             complement(28440..29087)
                     /gene="narP"
                     /locus_tag="OO433_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6049898.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPSFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         6683 a         7222 c         8105 g         7251 t
ORIGIN      
        1 atcaactact atggtgggcg cagcgaaacg gaattaacca ccgcccaact gacgctgatc
       61 accgatgaag gatcggtcaa tgagaaacag gaaacattta ttgtaccgat gcgtaatgct
      121 ggcgagctga cgctggtgaa aagttttgac tggtgatcca tcccttcaca caacctggct
      181 ctctcttttt tcagataaga ctgctgggcg atctgccgac gccaggatat aaccattaac
      241 aagcgtcggc agattggtca tttcttattt ttcgtactga gaactgtcca tctactccgg
      301 ctttcctggt taaggcaatg gtttctaagc gatacgcatt attgcaataa tgacagaaca
      361 ttgcggtaac acgcttttac cgctacctta accacactcc atcggtcacc tgaggcggag
      421 cttcgcccct ttgaaatacc ttgcttcttt tcgtacaacc ctgaaagcct cgcgctacat
      481 gttcagagca ttggcgttag tgctctggct gttgattgct ttttcatccg ttttttacat
      541 cgttaatgcg ttacatcagc gagaatcgga aattcgtcag gaatttaatc tgagttccga
      601 tcaggctcag cgctttattc aacgcacctc tgatgtgatg aaagagctga agtacatcgc
      661 cgaaaatcgc ttatcggcag aaaacggtgt gctttccccg cgtggacgag aaacgcaggc
      721 ggatgtgcct gcgtttgaac cgctgtttgc cgactccgat tgttccgcaa tgagtaacac
      781 ctggcgaggt tctctggagt cattggcgtg gtttatgcgc tactggcgcg ataatttttc
      841 tgcggcttac gatctaaacc gggtattttt aatcggcagc gataacctct gcatggccaa
      901 tttcggtctg cgtgatatgc cagtggaacg cgataccgcg ttgaaagctt tgcatgaacg
      961 catcaataaa tatcgaaatg caccacaaga tgatagcggc agtaacctct actggatcag
     1021 cgaaggtccg cgccctggcg tcgggtattt ttacgcgttg acgccagttt atctggcgaa
     1081 ccggttgcag gcgcttttgg ggggcgagca gaccatccgg atggagaact ttttcttacc
     1141 gggtacgttg ccgatggggg ttaccattct tgatgaaaat ggtcataccc tgatttcgct
     1201 taccggacca gaaagtaaaa ttaagggcga gcctcgctgg atgcaggaac gctcctggtt
     1261 tggctatacg gaagggttcc gggagctggt gctgaagaaa aatctgccac cctcatcgct
     1321 aagcatcgtg tattcggtgc cggttgataa ggtgctggaa cgcattcgca tgttgatcct
     1381 taacgcaatt ttgctgaatg tgcttgccgg agctgcattg tttactctcg cacggatgta
     1441 cgagcgacgt attttcattc cggcggaaag cgacgccctg cgactggaag aacatgagca
     1501 gttcaatcgc aagattgtcg cctccgcgcc agtgggtatc tgcattttgc gtaccgctga
     1561 tggcgtcaat attttaagta acgaactggc gcatacctat ctcaatatgc ttacgcatga
     1621 ggaccgccaa cgactgacgc aaattatctg tgggcagcag gtcaattttg ttgatgtcct
     1681 gaccagcaac aataccaatc tgcaaatcag cttcgtccat tcgcgctatc gtaatgaaaa
     1741 cgtggccatt tgtgtgctgg tggatgtttc ttcgcgcgtg aagatggaag agtcgttgca
     1801 ggagatggca caagcagcgg aacaggcgag ccagtcaaaa tcgatgttcc ttgccaccgt
     1861 cagtcatgag ctgcgaacgc cgctgtatgg cattatcggt aacctggatc tgttgcaaac
     1921 caaagagtta ccgaaaggcg tcgatcggct ggtgacggca atgaacaact cttccagcct
     1981 gttgttgaaa attatcagcg atattctcga tttctcgaag attgaatcgg aacagttgaa
     2041 gatcgaaccg cgtgagtttt caccgcgtga agtgatgaac cacatcaccg ccaactattt
     2101 accgctggtg gtacgcaagc agttaggctt gtactgcttt attgaaccgg atgtgccagt
     2161 ggccttaaat ggcgacccga tgcgtttaca gcaggtcatc tccaacctgt tgagtaacgc
     2221 cataaaattc accgataccg gctgtatagt tttgcatgtc cgcgctgatg gcgattatct
     2281 ctctatccgt gttcgcgata ccggcgtggg gattccggcg aaagaagtgg tgcgcttgtt
     2341 tgatcccttc ttccaggtcg gaacgggcgt acagcgaaac ttccagggga ccggtctggg
     2401 tctggcgatt tgtgaaaaac tgatcagcat gatggacggc gatatctcgg tagattcaga
     2461 accgggaatg ggcagccagt ttaccgtgcg tattccgttg tacggcgctc agtacccgca
     2521 gaaaaaaggc gtggaagggt tgagtggtaa acgctgctgg ctggcggtcc gcaatgcgtc
     2581 gctctgtcag ttcctagaaa ctagtttgca gcgcagcggc atcgtcgtta caacatacga
     2641 agggcaggaa ccgactcccg aagatgtgtt aattactgac gaggtagtga gtaaaaaatg
     2701 gcagggtaga gcggtagtga ccttctgtcg tcgccatatt ggtattccgc tggagaaagc
     2761 gccaggggag tgggtacaca gtgtggctgc cccgcatgag ctaccggcat tgttggcgcg
     2821 tatttatttg atcgagatgg agagcgacga tcctgctaac gctctgccgt cgacggacaa
     2881 agcggtcagc gataatgacg atatgatgat tctggtcgtg gatgatcatc cgattaaccg
     2941 acgtctgctg gcagatcagt tgggatcgtt gggctatcaa tgtaaaaccg cgaatgatgg
     3001 tgtcgatgcg cttaatgtac ttagcaagaa tcatattgat atcgtgctta gcgacgtcaa
     3061 catgccaaat atggatggtt accgcttgac gcaacgcatt cgtcagttgg gactgacgtt
     3121 gccggtaatc ggagtaactg ctaatgcgtt ggctgaagag aagcagcggt gtctggagtc
     3181 cggtatggac agctgcctgt cgaagccggt aacgctggat gtgataaaac agacgctgac
     3241 ggtatatgcc gagagggtca ggaaatcgcg ggaatcgtag gacggataag gtgtttatgt
     3301 cgcatctggc attcagtgca aatgccagat gcgacgctga cgcgtcttat ctggcctact
     3361 ttgatgtaat ctgttgaaat aatgggaatc gtaggacgca taaggcgttt acgccgcatc
     3421 cggcaatcgg tgcaaatgct agataagaca ctaacgcgtc ttatctggcc tacaggtgat
     3481 tagtctttat ctgccggact taacgtcact gaagagagat aattcagcag ggcgatatcg
     3541 ttctcgacac ccagcttcat catcgcagat ttcttctggc tactgatggt tttaatactg
     3601 cggttcagct ttttagcgat ctcggtcacc aagaagcctt ccgcaaacag gcgcagaact
     3661 tcactctctt ttggcgagag acgcttgtca ccgtaaccac cagcactgat tttttccaac
     3721 aggcgagaaa cgctttccgg ggtaaatttc ttccctttct gcagcgcggc gagagctttc
     3781 ggcagatcgg tcggtgcacc ttgtttcagc acgatccctt cgatatccag atccaatacc
     3841 gcactaagaa tcgccgggtt gttgttcata gtcagaacaa tgatcgacag gcttgggaaa
     3901 tggcgcttga tgtacttgat taaggtaatg ccatcgccgt acttatcgcc aggcatggag
     3961 agatcggtaa tcaacacatg cgcatccagt ttcggcaggt tgttgatcag tgctgtagag
     4021 tcttcaaatt cgccgacaac attcacccac tcaatttgct caagtgattt gcgaataccg
     4081 aacaagacta tcggatggtc atcggcaata attacgttca tattgttcat gtaataggct
     4141 accttgctac agcaagctct tgacataact gtcaatgtcg ctgatgtatt tttctattcc
     4201 tggaacatcc ttctcacgaa tcagatgttc cagcgtttca cataactgct tgccgggtac
     4261 cagatttagc atggcaaata cgcctttaag acgatgagcc gtttgggcta acgcagcaaa
     4321 gtcactggtt gctgcttcag tatacagcct cttaacatca tccggtactg tgtctacaaa
     4381 gagcgcataa tagccgctgg catggagttg cgcattttca tctccgccca gaggcgattc
     4441 tgtcacctct tcctgcgcca gttgcacttc aattaattgt aagaccgctt cctgcatagc
     4501 gttgctcata ttgaagttga cgcacaattg accaggccca atttcccgta cgccagactc
     4561 atcatcgctt aaaagcaagc cagaggcagt aagattagac ggattatccg ttaaaaagat
     4621 atcataatct tgactaatta atctttcatc gggtgtgata caggttgcac cccaattttc
     4681 taactggcga gtgacaatat tccgaatttc tgccgaagta acatccacca ttacgcagac
     4741 atcatccagt aaacgctctt cttcctcttc aacttccggg tcagcggcga gcattttgat
     4801 atgcacagag tagcgtgtac caagcccatc ccgcgttttg atgtttaaat gaccgcccag
     4861 tttacgcgcc agttgatcgc tcagccagaa tgccagcggg tccgccttgc catagcgatc
     4921 gttttggggc tggttgataa acgggaagtg caaattatcc atttcatgaa tacttacgcc
     4981 ttctcccgtg tccagaatgc ggaatgtcag gcggtcttca gaggactcat cctgatcaac
     5041 ctcaagggtg atttttccca attgcgttga ggtcacggca tattgcatca gtagcagcaa
     5101 aatacgtcgt aaggcatcgc gatcgccgcg gcgcatatcg tgtgctttca gatgattgtt
     5161 aatcagcagt tgcagacctt tacgcttgat ggcaggcaac actgaaggca caacttcatc
     5221 aattaaatcc tgcacggaga acagcaccgt ctcacttttc cagctatcgt ccgcaagcat
     5281 gttcgctaac tgtatttcat cgaccagccg caccagcaca tctgcctgat tcgccagttg
     5341 tttgctttcc ggggcgttga gtttagccgc gctctccgcc agggactgtg cgggttcttt
     5401 cagcgcatcg ccaatgtttt tcataaaggt catccgcccc tgctggtttt tctcatacag
     5461 acgctgcgcc tgcttgagtt tcttgtttac cagcacttcg cgatcctgat cgcgaataat
     5521 gaaaatttgt gtgcgcggcg cgacctggct gcggaacatg cggatctcat acagctcgtt
     5581 attgatcgtc gcctgaataa tcccctgatg ctgttccgcc atggtggtga tgttttgcag
     5641 attcaaatgc ggcagcaaat gatcggcaat tttgttactt atgacagtgc ggttcgattc
     5701 ctgatcgtga accagcaggc cgagcggcag cagtgagact atctcttcat taattgcccg
     5761 taaaatgcgc aattcgttat tgaccgccgt gctgggtaga ctttctgtac tgcggctgga
     5821 gaaatggcgg aatgtggtat agccaaataa cgccagcgcc agcaaaccga tgttcagcag
     5881 cagtggcagc agaatgtttt gcaacgtatc cagcaataag gtgccataag gaacctgcca
     5941 gaccaggcgc atatcggtag agttgagtgc cgaggagatt tcaatcttcg tactgttaaa
     6001 gtggatactg acgctatccg tcccttcttt ctcattatca ttgtctcccg ttgccgtcgc
     6061 gtctggctca aggcggaaac tgtccagcgg catacccggt gggatcaaat cattaatcgg
     6121 tagatcaaaa gctacgaccg ttgccagatg tcccggctgg ttgaacgtgg tacgcaaggt
     6181 aaagtaatga ccgttctgcc aggccaggcg gcgcatgtta gaaaaacttt cgcgttcatc
     6241 gagggcgttg gcctgttgca acatctctgc acgacgtgaa tcaacaatgt cgctgatggt
     6301 cgattcttta aatccggagg tgagatcttt gaggggcagg gttgagatca gcaccagact
     6361 gttatcctga ccattcaggt aatacatcga ccacggtaca ttttctgcgc cccacaatgt
     6421 gtccagatag gtggacatcc gctgagtcat ctcaagcgtt gagttgtcgt gagagccaaa
     6481 gattaacgct tccgttttgc ggcgcggttt ctccagatag tagacatcct gtttcaggcg
     6541 cgtctcttgt aaaccttcgc cggaggaggg ggaggttgtc gcggcaatgt tgtcgtagat
     6601 ctgccaggtc acgtaacgcc aggtattgac gcgcttttgg atagcgtggg taatgtcgac
     6661 aatctggtaa cttttatctt tcagccatgc gttaacggcg ctttgtacca ttacacccat
     6721 cgtcaccagt aacacaatga tcaacagtaa aaagaagcgg gtaatgctcc ccggtaggag
     6781 tgaaaagcgg gtcgtggccg ttgtctcttt ctgacgcatt cgtgtttatg acctgttaaa
     6841 acttcgcgaa gtgatagggc agtataaagg gtacagtgtg aaattccaga ctcttacctg
     6901 attgatgatt ctctattctc ccgtgatatt tatcatcctg cattggggga aatagatgta
     6961 caagcgccat tttgctgtac aaataaccta caaaaagccc aacgaaatta attgcaaata
     7021 aacgatgatt gctggaaatt atgctgatgt taattatttg tgaaatagtt aacaagcgtt
     7081 atagtttttc tgtggtagca cagaataatg aaaagtgtgt aaagaagggt aaaaaaaacc
     7141 ggatgcgagg catccggttg aaataggggt aaacagacat tcagaaatga atgacggtaa
     7201 taaataaagt taatgatgat agcgggagtt attctagttg cgagtgaagg ttttgttttg
     7261 acattcagtg ctgtcaaata cttaagaata agttattgat tttaaccttg aattattatt
     7321 gcttgatgtt aggtgcttat ttcgccattc cacaataatc ttaaaaagtt cccttgtatt
     7381 tacattttga aacatctata gcgataaatg aaacatctta aaagttttag tatcatattc
     7441 gtgttggatt attctgcatt tttggggaga atggacttgc cgactgatta atgagggtta
     7501 atcagtatgc agtggcataa aaaagcaaat aaaggcatat aacagagggt taataacatg
     7561 aaagttaaag tactgtccct cctggtccca gctctgctgg tagcaggcgc agcaaacgct
     7621 gctgaagttt acaacaaaga cggcaacaaa ttagatctgt acggtaaagt agacggcttg
     7681 cactatttct ctgacaacaa gtcagaagac ggcgaccaga cctatgtacg tcttggtttc
     7741 aaaggcgaaa ctcaggttac tgaccagctg accggttacg gccagtggga atatcagatc
     7801 cagggcaata cctctgaaga caacaaagaa aactcctgga cccgtgtggc attcgcaggt
     7861 ctgaaattcc aggatgtagg ttctttcgac tacggtcgta actacggcgt tgtttacgac
     7921 gtaacttcct ggaccgacgt actgccagaa ttcggtggcg acacctacgg ttctgacaac
     7981 ttcatgcagc agcgtggtaa cggcttcgcg acctaccgta acaccgactt cttcggtctg
     8041 gttgacggtc tgaactttgc tgttcagtac cagggcaaaa acggtagcgt aagcggcgaa
     8101 ggcatgacca acaacggtcg tggcgctctg cgtcagaacg gcgacggtgt cggcggttct
     8161 atcacttatg attacgaagg cttcggtatc ggtgctgcag tttccagctc caaacgtact
     8221 gatgatcaaa atggtagcta catcagcaat ggtgtagttc gtaactacat cggtactggc
     8281 gaccgtgctg aaacctacac tggtggtctg aaatacgacg ctaacaacat ctacctggct
     8341 gctcagtaca cccagaccta caacgcaact cgcgtaggtt ccctgggttg ggcgaacaaa
     8401 gcacagaact tcgaagctgt tgctcagtac cagttcgact tcggtctgcg tccgtctgta
     8461 gcatacctgc agtctaaagg taaaaacctg ggtgtcatca atagtcgtaa ctacgacgac
     8521 gaagatatcc tgaaatatgt tgatgttggc gcgacctact acttcaacaa aaacatgtcc
     8581 acctatgttg actacaaaat caacctgctg gacgacaacc agttcactcg tgacgctggc
     8641 atcaacactg ataacatcgt agctctgggt ctggtttacc agttctaatc tcgattgata
     8701 tcgaacaaag ggcctgcggg cccttttttc attgttttca gcgtacaaac tcagtttttt
     8761 ggtgtactct tgcgaccgtt cgcatgagga taatcacgta tggaaataag ctttacccgc
     8821 gtggcactgc tggctgccgc gctcttcttt gttggttgcg atcaaaaacc acaacccgcc
     8881 aaaacccacg ctactgaagt taccgttctt gaaggcaaaa ccatgggtac cttctggcgt
     8941 gccagcatcc cgggcattga tgccaaacgc agtgccgaac ttaaagaaaa gattcagacc
     9001 cagctggacg ccgacgatca gctgctttcg acctataaaa aagattccgc gctgatgcgc
     9061 tttaacgact cgcaaagttt gtcgccgtgg ccagtaagtg aagcgatggc cgaaatcgtc
     9121 actacctcgc tgcgcattgg cgcgaagacc gatggtgcga tggatattac cgtcgggccg
     9181 ctggtaaatc tgtggggctt tggcccggaa caacagccgg ttcaaattcc gagccaggaa
     9241 cagatcgacg agatgaaagc caaaactggt ttacagcacc ttacggtcat taatcagtcg
     9301 catcagcaat atctgcaaaa agacctgccg gatttatatg tcgatctctc taccgtcggc
     9361 gaaggttatg cggcggatca cttggcacgc ttgatggagc aggaagggat ttcccgctat
     9421 ctggtgtcgg tgggcggcgc gctgaacagc cgtggtatca acggtgaagg cctgccgtgg
     9481 cgggtagcga ttcaaaaacc aaccgataaa gaaaacgcag ttcaggctgt ggtggatatc
     9541 aacggtcatg ggattagcac ctctggcagc taccgtaact attacgaact ggacggcaaa
     9601 cgtctttccc atgttatcga tccgcaaacc gggcgtccca tcgaacacaa tctggtatcc
     9661 gtgacggtga ttgctccgac ggcgctggaa gccgatgcct gggatactgg cttgatggta
     9721 ctcgggccgg agaaagccaa agaggttgtt cgccgggaag ggctggcggt ttatatgatc
     9781 accaaagaag gcgatagctt taaaacctgg atgtcaccac agtttaaaag cttccttgtc
     9841 agcgaaaaaa attaaagcgc aagattgttg gtttttgcgt gatggtgacc gggcagacta
     9901 aaggctatcc ttaaccaggg agctgattat gaaaaacgcc acatgcttaa ctgacgatca
     9961 acgctggcaa tctgtcttag cccgcgaccc gaatgccgac ggcgaattcg ttttcgccgt
    10021 gcgcaccaca ggcatctttt gccgtccgtc ttgccgcgcc agacatgcct tgcgggaaaa
    10081 tgtctccttc tacgcaaatg ccagcgaggc actcgccgcc ggatttcgcc cctgcaaacg
    10141 ctgccaacct gataaagcca atgcccagca acatcggttg gataaaatca cccacgcgtg
    10201 tcgactgctg gaacaggaaa cgcctgtaac gttggaagcc ttagccgacc aggtggcgat
    10261 gagtccattt catctacatc ggttgtttaa agcgactacc ggaatgacgc ctaaagcctg
    10321 gcaacaggcc tggcgcgctc gccgtttgcg cgaatcgctg gcgaaagggg agagcgtgac
    10381 gacgtctatt cttaacgccg gattccccga cagcagcagt tactaccgca aagccgatga
    10441 aacgctgggc atgacggcta aacaattccg tcacggtggc gaaaatctgg cggtgcgtta
    10501 cgcgctggct gattgtgcgc tgggtcgttg cccggtggca gaaagcgagc gggggatttg
    10561 cgcgatattg ctgggcgatg atgacgcgac attaatcagc gagttgcagc agatgtttcc
    10621 cgctgccgac aacgcgcctg ccgatctgac gtttcagcaa catgtgcgtg aagtgatcgc
    10681 cagcctcaat caacgcgata cgccgctgac gttaccgctg gatattcgcg gcactgcttt
    10741 tcagcaacaa gtctggcagg cactgcgcac gataccttgc ggtgaaaccg tactggctaa
    10801 cgccatcggc aaaccgaaag cggtacgggc cgttgccagc gcctgtgccg ccaacaagct
    10861 ggctatcgta ataccctgtc atcgagtggt cggtggtgat ggcacacttt ccggttaccg
    10921 ctggggcgtg tctcgtaaag cgcaactgct gcgccgcgaa gctgaaaatg aggagaggta
    10981 atgttggatc tgtttgccga tgctgaaccg tggcaagagc cactggcggc tggtgcggta
    11041 attttacggc gttttgcttt taacgctgcg gagcaactga tccgcgatat taatgacgtt
    11101 gccagccagt cgccgtttcg ccagatggtc acccccgggg gatataccat gtcggtggcg
    11161 atgaccaact gtgggcatct gggctggacg atccatcggc aaggttatct ctattcgccc
    11221 attgatccgc aaacaaataa accgtggccc gccatgccac aaagctttca tgatttatgt
    11281 caacgtgcgg ctacggcggc gggctatcca gatttccagc cagatgcttg tcttatcaac
    11341 cgctacgctc ctggcgcgaa actgtcgctg catcaggata aagacgaacc ggatctgcgc
    11401 gcgccaattg tttctgtttc tctgggctta cccgcgattt ttcaatttgg tggcctgaaa
    11461 cgtaatgatc cgctcaaacg tttgttgttg gaacatggcg atgtggtggt atggggcggt
    11521 gaatcgcggc tgttttatca cggtattcaa ccgttgaaag cggggtttta tccactcacc
    11581 accgactgcc gctacaacct gacattccgt caggcaggta aaaaagaata aaaataagaa
    11641 ttattattgc tgtgcgcgcg aagatgttta aactgcgggc tgtaattcat tgtccgggtt
    11701 ttctgcatgg aacttcttgt acttgtctgg cggcagtatc gctggccatt tatcagtgtg
    11761 atggcgctaa gcctcgccag tgcggcatta ggcattggct taattgcttt tatcaatcag
    11821 cgccttatcg aaatggcgga taccagtctg ctggtattgc cggagtttct gggattattg
    11881 ctgctgttga tggcagtcac tctcggatcg caactggcgc tcaccacttt ggggcatcac
    11941 ttcgtttacc gactgcgtag tgaatttatc aagcggattc tggataccca cgtcgagcgc
    12001 attgaacaac tcggtagcgc ctcgttgttg gcgggattaa ccagcgatgt gcgcaatatc
    12061 accattgctt ttgtgcgtct tccggaactg gtgcagggga tcattctcac tatcggttcc
    12121 gcggcgtatc tgtggatgct gtcgggcaaa atgttgctgg taacggctat ctggatggcg
    12181 atcaccatct ggggtggttt tgtactggtg gcgcgggtgt acaaacatat ggcgacccta
    12241 cgtgaaaccg aagacaagct gtacacggat tttcaaactg tacttgaagg gcgcaaagag
    12301 ctgactctga accgggaacg cgccgagtat gtgtttaaca acctctacat ccccgatgcg
    12361 caagagtatc gccatcatat tattcgcgca gacaccttcc atcttagtgc cgtgaactgg
    12421 tcaaacatca tgatgctggg cgctatcggc ctggtgttct ggatggcgaa cagtctcggt
    12481 tgggctgata ccaacgttgc tgcgacctat tcgttgacgc ttttattcct gcgtacgccg
    12541 ctgctttcgg cggttggcgc attgccgacg ctgctgatgg cgcaggtggc gtttaacaag
    12601 ctgaacaaat tcgcgctcgc gcctttcaaa gcagagtttc cgcgcccgca ggcgtttccc
    12661 aactggcaaa cgctggagct gcgtaatgtg acgtttagct atcaggataa cgcgttttcc
    12721 gttggtccga ttaatctcac catcaaacgt ggcgagctac tgtttctgat tggtggtaac
    12781 ggtagtggaa aatcgacgct ggcgatgttg ttgacgggct tgtatcagcc acaaagcggc
    12841 gaaatcttgc tggatggcaa acctgtcagc ggcgaacaac cggaagatta tcgcaaactg
    12901 ttttcggcag tgtttaccga tgtctggctt tttgatcaac tgctggggcc ggagggtaaa
    12961 cccgctaacc cgcaactggt tgagaagtgg ctggcgcagc tgaaaatggc tcataagctt
    13021 gagttaagca acgggcgtat tgttaacctg aagttatcaa aagggcagaa aaaacgcgtg
    13081 gcgctgttgc tggcgctggc agaagaacgc gatattatcc tgctggatga atgggcggcg
    13141 gatcaggatc cacacttccg tcgtgagttt tatcaggtgt tgctgccgct gatgcaggag
    13201 atgggtaaaa ctattttcgc tatcagtcat gatgatcatt actttatcca cgccgaccgc
    13261 ctgctggaaa tgcgcaatgg gcaacttagc gagctgacgg gcgaagagcg cgatgccgct
    13321 tcgcgtgatg ccgttgcccg gacggcataa tattacgacg ctgacctggc ggactgctgc
    13381 cgtcaggtca atatgctaaa aatcccttca tttctaataa ccctataatt aatcaacgaa
    13441 attataatgt ttctaaaatt agaatataat ttataaacat tatttaaatg ttgttactta
    13501 agtgttacct ttgatgccgc gcaaatctca cctgcaataa agcgactaaa agtaaggcat
    13561 taacaagatg aaaaaagtga ctgccatgct cttctcgatg gccgtggggc ttaatgccgt
    13621 ttcgatggcg gcaaaagcga aagcgtccga ggagcaggaa actgatgtac tgttgattgg
    13681 cggcggcatt atgagcgcca cgttggggac ctatttacgc gagctggagc ctgaatggtc
    13741 gatgaccatg ctggagcgcc tggagggtgt cgcgcaggag agttcgaacg gctggaataa
    13801 cgccggaacc gggcattctg cactgatgga actgaactac accccgcaaa acgccgatgg
    13861 cagtatcagt attgaaaaag cagtcgccat taacgaagca tttcagattt cccgccagtt
    13921 ctgggcgcac caggtcgagc gcggcgtgct gcgtactccg cgttcattta tcaataccgt
    13981 tccgcatatg agctttgtct ggggcgagga taacgttaat ttcctgcgcg cccgttacgc
    14041 cgcgttgcaa caaagctcgc tgtttcgcgg tatgcgttac tctgaagatc acgcgcagat
    14101 caaagagtgg gcaccgttag tgatggaagg gcgcgatccg caacagaaag tggcagccac
    14161 gcgtacggaa attggtaccg atgtgaacta cggcgagatc acccgccagt taattgcttc
    14221 cttgcagaag aaatctaact tctcgctgca actcagcagc gaagtccgcg ccctaaagcg
    14281 taatgacgat aacacctgga ccgttaccgt tgccgatctg aaaaatggca ctgcacagaa
    14341 cattcgtgcc aaatttgtct ttatcggcgc gggcggtgcg gcgctgaagc tgttacagga
    14401 atcggggatt ccggaagcga aagactacgc cggtttcccg gtgggcggac agttccttgt
    14461 ttcggaaaac ccggacgtgg ttaatcacca tctggcgaag gtttacggta aagcatccgt
    14521 tggcgcacca ccgatgtcgg ttccgcatat cgatacccgc gttctggacg gtaaacgcgt
    14581 agtgctgttt gggccatttg ccaccttctc aaccaaattc ctcaaaaacg gttcattgtg
    14641 ggatctgatg agttccacca ccacctctaa cgtgatgccg atgatgcacg tcgggctgga
    14701 taatttcgat ctggtgaaat atctggtgag tcaggtgatg ttgagtgaag aggatcgttt
    14761 tgaagcgttg aaagagtact atccgcaggc gaaaaaagag gactggcgtt tgtggcaagc
    14821 ggggcagcgt gtgcagatta tcaagcgtga tgccgataaa ggtggtgtac tgcgtctggg
    14881 tactgaagtg gtcagtgacc agcaagggac cattgccgcg ctcctgggag catcgccagg
    14941 ggcgtcaacc gccgcgccga ttatgttgga tctgctggaa aaagtatttg gcgatcgcgt
    15001 ttccagcccg caatggcagg ctacgttgaa agcgatcgtt ccgtcttatg gacgcaagct
    15061 gaacggtgat gtagcggcaa cagaacgcga gttgcagtac accagcgaag tgctggggct
    15121 gaaatacgac aagccgcagg cagcagatag tacgccaaaa ccgcagttga aaccgcaacc
    15181 cgttcaaaaa gaagtggcgg atattgcgtt gtaatgatgt gccacatccg gcatggtatg
    15241 ccggatgtgg cgtatactga taagacgcgc cagcgtcgca tcaggcaacc ggctcgggcg
    15301 ttagatgttg gatgcggcgt aaccgcctta tccaaccaac ggggtgccgt acttcacaac
    15361 agtttagcga acttctgcgt tgtcaatttt ctcttccgcc ttccagacgc ggtatttcac
    15421 atctacattg tctggcgtat acaccacgat cggcagcttg ctgttgtaac gcagcattcc
    15481 agcatcgccc agatacgcgg tgacaaattt cttctctttc ttgccatccg ggcaggccat
    15541 catcgtggaa accggcgagc taactttatc aaagacataa taatcatagc cccagccttc
    15601 cagcgttttg ctttccagct tcccgccgag acgatgcaaa ttgcaatcga cttccagcgt
    15661 ctgaccgatt aacagttcta ctttcagggt agattcatct tcttgcgggg ttaactgaat
    15721 cacctgacgc ttcatccctt tttcagcttg tggataaggc gcgatttttt ccagtggctg
    15781 gacgctttct gccgcccagg cggaagtggt agcgaaagcg gcaaacaata ctgcaggtag
    15841 aatggtcttc atttttttgc tttccttctt catttatagg ttttcaccga tgatgtcgct
    15901 gaaggttaac atattcataa gatttattaa tttgctttat gaataactta aagaatattc
    15961 agacaatttt acctgctctt ttagtgttaa attctgatga gagaacaaac cagcagaaat
    16021 atctctggtg aattaatcgc tatataaata tgtttataac catttgaaat gtgagcaaaa
    16081 gcccgttttt gccacgcttc ccgccactct tttgatcctg ctacaggttt taccccgatc
    16141 ggggtatgca tctttgacac atcctttaat atcttagcgg ttataaaaat ggcttattaa
    16201 ttatgcggtt tatttggtcg ctctcaattt tcagagcgcg ttaatgatgg aaggtcaatg
    16261 tgaagattga tgcatcccgt cggggcatac tcactggtcg ctggcgcaaa gccagtaacg
    16321 gtatccgtcc gccctggtcc ggtgatgaat ctcattttct gacccattgc acccgttgcg
    16381 acgcctgtat taatgcttgt gaaaacaaca ttctgcaacg cggcgcgggc ggctatccga
    16441 gcgttaattt caaaaataac gagtgcagct tctgttacgc ttgcgcgcag gcttgccccg
    16501 aatcgctatt ttctccgcgc cacaccaggg cctgggattt gcagttcacc attggggacg
    16561 cgtgcctggc gtatcagtca gttgaatgcc gccgctgtca ggatagctgt gagccaatgg
    16621 caattatctt tcgcccgacg ctctccggga tctaccagcc gcaacttaat agccaacttt
    16681 gcaacggatg tggagcatgt gcagccagct gtccggtatc agccataact gcggagtatc
    16741 tccatgcaca ctaactggca agtttgcagc ctggtcgtgc aggccaaaag cgaacgaatt
    16801 tcagacatca gcacccaact gaacgccttt cccggctgtg aagttgctgt cagcgacgcg
    16861 ccgagcggtc agttgattgt ggtggtggaa gcagaagaca gcgaaacgct gatccaaacc
    16921 attgagtcag tacgcaacgt agagggcgtg ctggcggtgt cgctggttta tcaccagcag
    16981 gaagagcaag gtgaggaaac accatgaaac tcagtcgtcg tagctttatg aaagctaacg
    17041 ccgttgcggc cgctgcggcg gctgccggtc tcagcgtgcc gggcgttgcc cgcgccgttg
    17101 ttggtcagca ggaagccatc aaatgggata aagcgccgtg ccgtttctgc ggtactggtt
    17161 gcggcgttct ggtcggaacg cagcagggac gtgtggtggc ctgtcagggc gacccagacg
    17221 caccggttaa ccgtggcctg aactgcatta agggctattt cctgcccaag atcatgtacg
    17281 gtaaagaccg tttgacgcag ccgctgctgc gtatgaaaaa cggtaaatat gacaaagaag
    17341 gcgaatttac cctaatcacc tgggatcagg ccttcgatgt gatggaagag aaattcaaaa
    17401 ccgccctgaa agaaaaaggg ccggaatcga tcggtatgtt cggttctggt cagtggacta
    17461 tctgggaagg ttatgccgcg tccaagctgt ttaaagcggg cttccgttcg aacaacatcg
    17521 acccgaacgc gcgtcactgt atggcgtcgg cagtagttgg ctttatgcgt acctttggta
    17581 tggatgagcc gatgggctgc tatgacgaca tcgagcaggc tgacgcgttt gtgctgtggg
    17641 gcgcaaacat ggcggagatg cacccgatcc tctggtcgcg catcactaac cgtcgtctct
    17701 ctaatcagaa cgtcaccgtg gcggtgcttt ctacctacca gcatcgtagc ttcgagctgg
    17761 cggataacgg catcatcttt acgccacagt ctgacctggt gatcctgaac tacatcgcca
    17821 actatatcat tcaaaacaat gcgataaatc aggacttctt cagcaaacac gttaacctgc
    17881 gcaaaggggc gacggacatt ggctacggtt tacgtccgac ccatccgctg gaaaaagcag
    17941 cgaagaatcc aggttctgac gcctccgaac cgatgagctt tgaagattac aaagccttcg
    18001 ttgccgagta tacgctggaa aaaactgccg aaatgaccgg cgtgccgaaa gaccagttag
    18061 aacaactggc gcagctgtat gccgatccga acaagaaagt catctcctac tggacgatgg
    18121 gcttcaacca gcatactcgt ggcgtgtggg ctaacaacct ggtctacaac ctgcacctgc
    18181 tgaccggcaa aatttcccag ccgggttgcg gtccgttctc cctgaccggg caaccttctg
    18241 cgtgtggtac tgcgcgtgaa gtgggcacct ttgctcaccg tctgcctgcg gacatggtgg
    18301 tgaccaacga gaaacaccgt gatatctgcg agaagaagtg gaatatcccg agcggcacca
    18361 ttccggcgaa aatcggtctg cacgcggtgg cacaagaccg tgcgctgaaa gacggcaaac
    18421 tgaatgttta ctggaccatg tgtaccaaca acatgcaggc cgggccgaac atcaatgaag
    18481 agcgtatgcc gggctggcgc gatccgcgca acttcatcat cgtctccgat ccgtatccga
    18541 cagtcagtgc gctggccgcc gacttgatcc tgccgaccgc aatgtgggta gagaaagagg
    18601 gcgcttacgg taacgccgaa cgccgtactc agttctggcg tcaacaggta caggcaccgg
    18661 gcgaagcgaa atcggatctc tggcagttag tccagttctc ccgccgcttc aaaactgaag
    18721 aagtatggcc ggaagatctg ctggcgaaga aaccggaact gcgtggcaaa acgctgtacg
    18781 aagtgctgta tgccacaccg gaagtgagca aattcccggt atccgaactg gcggaagatc
    18841 agctgaacga tgaatcccgc gagctgggct tctatctgca aaaagggctg ttcgaagagt
    18901 acgcatggtt tggtcgcggt cacggtcacg atctggcacc gttcgatgac taccacaaag
    18961 cgcgcggtct gcgctggccg gtggtgaacg gtaaagaaac gcagtggcgt tacagcgaag
    19021 gtaacgaccc gtacgtgaaa gcgggcgaag gctataagtt ctacggcaaa ccggatggca
    19081 aagcggtgat cttcgcactg ccattcgaac cggcggcgga agcaccggat gaagagtacg
    19141 acctgtggct ctctaccgga cgcgttctgg agcactggca caccggcagt atgactcgcc
    19201 gtgtaccaga actgcaccgc gccttcccgg aagcggtcct gtttattcac ccgctggatg
    19261 cgaaagcgcg cgatctgcgt cgtggcgaca aagtgaaagt ggtttctcgc cgtggcgaag
    19321 tgatctcgat tgtcgaaacg cgcggtcgta accgtccgcc gcagggcctg gtgtacatgc
    19381 cgttcttcga cgccgcacag ctggttaaca aactaacgca ggacgcgacc gatccgctct
    19441 cgaaagagac ggacttcaag aagtgcgcag tcaaactgga gaaggtgtaa gccattatgt
    19501 cccggtcagc gaaacctcaa aatggtcgcc gccgctttct gcgcgatgtt gttcgcacag
    19561 caggcgggct ggctgccgtg ggtgtggcgc tggggttaca acagcaaacc gcacgcgcat
    19621 ctggcgtgcg gttgcgcccg cccggagcca taaacgagaa cgcctttgcc agtgcctgtg
    19681 tgcgttgcgg tcagtgtgtt caggcttgcc cttacgacac cttaaaactg gcgacgctgg
    19741 cctctggtct gtcggcgggc acgccatatt ttgtcgcacg ggatattcct tgcgaaatgt
    19801 gtgaggacat tccgtgcgcc aaagtgtgcc cgagcggtgc gctggatcgt gagatcgaat
    19861 cgatcgacga cgcgcggatg gggctggcgg tactggtgga ccaggaaaac tgtctcaact
    19921 ttcaggggct gcgctgcgat gtttgttatc gcgaatgccc gaaaattgat gaggccatca
    19981 ccctggagct ggagcgcaac acgcgtaccg gtaagcacgc ccgctttctg ccgacggttc
    20041 acagagacgc ctgtaccggt tgcggtaagt gcgaaaaagt gtgcgtgctg gaacaaccgg
    20101 caatcaaggt gttaccgctg tcactggcta aaggggagtt aggtcaccat taccgcttcg
    20161 gctggctgga ggggaacaat ggcaaatcgt aaacgtgacg ccgggcgcga ggcgctggag
    20221 aaaaaaggct ggtggcgcag tcaccgttgg ctggtgttac gtcgcctttg tcagttcttc
    20281 gtgctgggga tgtttttgag cggtccgtgg tttggtgtgt ggatcttaca cggtaactac
    20341 agcagtagcc tgttattcga caccgttccg ctgaccgatc cgctgatgac gctgcaaagt
    20401 cttgccagcg gtcatctgcc tgccacggtg gcgttgaccg gtgcggtgat tatcaccgtg
    20461 ctctacgccc tggcaggaaa gcgattattt tgcagctggg tctgcccgct gaatccgatt
    20521 accgacttag cgaactggtt acgcaggcgc tttgacctca atcagtctgc gacgatccct
    20581 cgccacattc gctacgtgct gttggtggtc attctgctgg gatcggcctt aactggcacg
    20641 ctcatctggg aatggatcaa ccccgtatct ctgatggggc gcagcctggt aatgggcttc
    20701 ggcagcggcg cgctgcttat tctcgcgctg tttttatttg atctactggt cgttgaacac
    20761 ggctggtgcg ggcacatttg tccggttggc gcgctgtatg gcgtgctggg tagcaaaggt
    20821 gtgattaccg ttgcggccac cgatcggcag aaatgtaacc gctgtatgga ttgttttcat
    20881 gtttgcccgg aaccgcgtgt gctacgtgcc ccggtgctgg atgaacaaag cccggtgcag
    20941 gtcaccagcc gcgattgcat gacgtgcggt cgctgcgtgg atgtctgttc tgaggatgta
    21001 tttacaataa ctacacgatg gagttcggga gcgaaatcat gaaaagccat gacctgaaga
    21061 aagcgctgtg tcaatggacg gcgatgctgg ccctggtggt aagcggcgcg gtttgggcgg
    21121 ctaatggcgt cgattttagc cagtcgccgg aagtatccgg gacacaggaa ggggccattc
    21181 ggatgccaaa agagcaggaa cggatgccgc tgaactatgt gaatcagccg ccaatgatcc
    21241 cgcatagcgt tgaaggttat caggtaacga ccaataccaa ccgctgcttg cagtgccacg
    21301 gtgtcgaaag ttatcgcacc actggcgcgc cgcgcattag tcctactcac tttatggaca
    21361 gcgacggcaa agtgggcgcg gaagtggctc cgcgtcgtta tttctgtctg caatgtcacg
    21421 taccgcaggc cgataccgcg ccaatcgtgg ggaatacctt taccccatca aaaggttacg
    21481 ggaaataaga ggttattatg ggaaattctg accgtaagcc tggtctgatt aagcgcctgt
    21541 ggaaatggtg gcgtaccccc agccgtctgg cgctggggac gctgctgttg atcggttttg
    21601 ttggcggcat cgtcttctgg ggtggcttta acaccgggat ggaaaaagcc aataccgaag
    21661 agttctgcat tagctgccac gaaatgcgca acacggtgta tcaggaatac atggattccg
    21721 tgcactacaa caaccgtagc ggcgtccgtg cgacctgtcc ggattgtcac gttccgcacg
    21781 agtttgtgcc gaagatgata cgcaagctca aagcaagtaa agagctgtat ggtaaaattt
    21841 ttggcgttat tgacacgccg cagaaatttg aagctcatcg tctgacgatg gcacagaatg
    21901 agtggcggcg catgaaggac aataactcgc aggagtgccg taactgtcac aacttcgagt
    21961 atatggatac aaccgcccag aaatcggttg ccgcgaagat gcatgaccag gcggtgaaag
    22021 atgggcaaac ctgtattgat tgccataaag ggatagcgca caagctgccc gatatgcgtg
    22081 aagtcgagcc aggtttttaa cagggttatt gcgtgggtat gcttgaagcc agagagttac
    22141 tttgtgagcg ggatgaacga accttattta gtggcttgtc atttacgctg aacgcaggag
    22201 agtgggtaca aatcaccggt agcaacggcg cggggaagac aacgcttctc cgtttgctga
    22261 cggggttgtc tcgccctgac gcaggcgagg ttctctggca agggcagccc ttgcatcagg
    22321 tacgcgacag ctaccatcaa aacctgttat ggataggcca tcagccgggg atcaaaaccc
    22381 ggctgacggc gttagaaaat ctgcactttt atcatcgcga tggcgatacc gcacaatgtc
    22441 tggaagccct ggcgcaggcc gggcttgccg gattcgaaga tattcctgta aatcagctct
    22501 cggccgggca acaacgccgc gtcgctttag cgcgtctgtg gctgacccgt gccacgttat
    22561 ggatcctcga cgagcctttt accgcgattg acgttaacgg cgtcgatcgt ctgacccagc
    22621 gtatggcgca gcatacggag caggggggga ttgtgattct gactacccac cagccgctca
    22681 acgttgctga aagtaaaatt cgccgcattt cactgacgca aacgagggcc gcatgatgtt
    22741 ctggcgcatt ttccgtcttg agctgcgtgt agcgtttcgc catagcgccg aaatcgccaa
    22801 cccgctgtgg ttcttcctga ttgtaattac cctttttccg ctcagtatcg gtccggagcc
    22861 gcaactgctg gcgcgtattg caccgggcat tatctgggtt gctgcgctgc tttcatcctt
    22921 gctggcgctg gaacgactgt tccgtgacga tttgcaggac ggcagtcttg aacaattgat
    22981 gttgttgccg ttacccttgc ccgccgttgt gctggcgaag gtgatggcgc actggatggt
    23041 aaccggtctg ccgttactca tcctttcgcc actggtagca atgctactgg gaatggatgt
    23101 ttatggctgg caagtgatgg cgctgacgct gctgctggga acgcctacgc ttggctttct
    23161 cggtgcaccg ggtgtggcgt tgacagtggg acttaagcgc ggtggtgtgc tgctcagcat
    23221 actggtgtta ccgctgacta tcccattact catctttgcc accgccgcga tggacgcggc
    23281 ttccatgcat ttgcccgttg acgggtatct ggcaatttta ggcgcgttgc tggcaggcac
    23341 cgcgacatta agtccttttg cgacggcggc agcgttacga atcagcattc aataacgctg
    23401 gattactgat ttttgttcga gtctggtatc gaaactatgt ggaaaacact gcatcaactg
    23461 gcgatcccac cacggctgta tcaaatctgt ggctggttta taccgtggct ggcaattgcc
    23521 agtgtggtcg tgcttaccgt cggctggatc tggggattcg gctttgctcc ggctgattat
    23581 cagcagggaa atagctaccg cattatctac ctgcatgtgc ctgcggcgat ctggtcgatg
    23641 ggtatttatg catcaatggc ggtggcggcg tttattggcc ttgtctggca gatgaaaatg
    23701 gccaacctgg cggtggcggc gatggccccc attggtgccg tgtttacctt tattgccctg
    23761 gttaccggct ctgcatgggg aaaaccgatg tggggcacct ggtgggtatg ggatgcacgt
    23821 ctgacttctg aactggtgct gctgtttttg tatgtgggtg tgattgccct gtggcacgcc
    23881 ttcgacgacc gccgtctggc gggccgtgcg gcaggtatcc tggtgctgat tggcgtggtg
    23941 aatctgccga ttattcatta ctccgtggag tggtggaaca ccctgcatca gggatcaacg
    24001 cggatgcagc aaagtatcga tccggcgatg cgttcgccgc tgcgctggtc gatttttggc
    24061 ttcctgctcc tgtctgccac gctgacgctg atgcggatgc gtaatttgat tttgctgatg
    24121 gaaaaacgcc gtccgtgggt gagtgaactg atactgaaaa gaggccgtaa atgacccttg
    24181 catttgcttc ctggaatgaa tttttcgcaa tgggcggtta cgcctttttt gtctggctgg
    24241 cggtggtgat gaccgttatt ccgctggtgg ttttggtcgt gcactcggtg atgcagcatc
    24301 gcgcaattct gtgtggtgtg gagcaacagc gggcgcgtga ggcgcgttta cgtgctgcgc
    24361 aacagcagga ggctgcatga atattcgccg taaaaaccgc ttgtggattg cctgtgctgt
    24421 gttggcaggt ttggcgctga ccatcggtct ggtgctgtat gcgctgcgct cgaatatcga
    24481 tctcttttat acgccggggg aaattctcta cggcaagcgt gaaactcagc aaatgccgga
    24541 agtcggtcag cgtctgcgcg ttggcgggat ggtgatgccg ggtagtgtgc agcgcgatcc
    24601 caattcgctg aaagtgacct tcaccattta cgacgctgaa ggctcagtgg atgtctctta
    24661 cgaaggcatt ttgccggatc tgttccgtga agggcagggc gttgtggtgc agggcgagct
    24721 ggaaaaaggc aatcatatcc tcgcgaaaga agtgctggcg aaacacgacg aaaactacac
    24781 gccgccagaa gttgagaaag cgatggaagc taaccaccgt cgcccggcga gtgtttataa
    24841 ggacccagca tcatgatgcc ggaaattgga aacgggctgc tgtgcctggc gctgggaatt
    24901 gcgctgctgc tgtccgtgta tccgctatgg ggcgtggcgc gcggagatgc gcgcatgatg
    24961 gcgtcttccc gcttgtttgc ctggatgctg tttatgtctg tggctggcgc atttctggtg
    25021 ctggtcaatg ctttcgtggt caacgacttt accgtcacct atgttgccag caactccaat
    25081 acccagcttc cggtgtggta tcgcgtggcg gctacctggg gcgcgcatga aggttcgctt
    25141 ctgctgtggg tgctgctgat gagcggctgg acctttgcgg tggcgatttt tagtcagcgt
    25201 attccgctgg atattgtggc ccgtgtactg gcgataatgg ggatggtcag cgtcggcttt
    25261 ttgctgttca ttctctttac ctctaacccg ttctcacgca cgctgccgaa cttcccaatt
    25321 gaagggcgcg atcttaaccc gctgttacag gatccggggc tgatcttcca tccgcctctg
    25381 ctttatatgg ggtacgtggg tttctcggtg gcgtttgctt ttgccattgc cattgcttct
    25441 ttgctgagcg ggcgtctgga cagcacttat gcgcgtttta ctcgcccgtg gacgctggcg
    25501 gcgtggattt tcctgacgct cggcattgtg ctcggttccg catgggccta ttacgaactc
    25561 ggctgggggg gctggtggtt ctgggatccg gtagaaaacg cctcgtttat gccgtggctg
    25621 gtggggactg cgctgatgca ctcactggcg gtcactgaac aacgcgccag cttcaaagcg
    25681 tggacattac tgctggcaat cagtgccttc tcgttgtgtc tgctggggac tttcctggtg
    25741 cgttccggcg tgctggtatc ggtacacgcg tttgcctctg atccggcacg cggtatgttt
    25801 atcctcgcct ttatggtact ggtgattggt ggttcgctgc tgctgtttgc cgcgcgtgga
    25861 cacaaagttc gctcacgcgt aaacaatgcg ctgtggtcgc gggaatctct gctgttagcg
    25921 aacaacgttt tgttggtcgc cgcgatgctg gtggtattac tggggacgct gctgccgctg
    25981 gtgcacaagc aactgggact gggcagtatt tcgattggcg aaccgttctt caacaccatg
    26041 tttacctggc tgatggtgcc gtttgcgctg ctgcttggtg tcggtcctct ggtgcgctgg
    26101 gggcgcgatc gcccacgtaa aatccgcaat ttattgatta tcgccttcat ctcaacgctg
    26161 gtgctgtcgc tgctgctgcc gtggctgttc gaaagcaaag ttgtggcgat gacggtgctc
    26221 ggcctggcaa tggcctgctg gattgctgtg ctggcaattg cggaagctgc gctgcgtatt
    26281 tcacgtggca cgaaaaccac cttcagttat tggggaatgg tggcggctca cctggggctg
    26341 gcagtgacaa ttgttggtat tgcctttagc cagaactata gcgttgagcg tgatgtgcgc
    26401 atgaagtccg gcgatagcgt cgatattcat gaatatcgct tcaccttccg tgatgtcaaa
    26461 gaggtgactg gcccgaactg gcgcggcggt gtggcgacta ttggcgtaac gcgagagggt
    26521 aaaccggaaa cggtgctgta tgcggaaaaa cgttattaca acactgccgg gtcgatgatg
    26581 accgaagcgg cgattgacgg cggcatcacg cgtgacctgt acgccgcgct cggtgaagag
    26641 ctggaaaacg gcgcgtgggc cgtgcgtctt tactacaaac cgtttgttcg ctggatttgg
    26701 gcgggcgggc tgatgatggc gttgggcgga ctgctgtgtc tgtttgatcc tcgctatcgt
    26761 aagcgcgtga gtccgcaaaa aactgcgccg gaggctgtat gaagcgcaaa gtattgttaa
    26821 ttccgttgat tatcttcctg gcgattgccg cggcgctgct gtggcagctg gcgcgtaatg
    26881 ccgaagggga tgatccgacc aatctggaat cggcgctcat tggcaagcca gtgccgaagt
    26941 ttcgcctcga atcactggac aatccggggc agttttatca ggcggatgtg ctgactcagg
    27001 gcaaacctgt gctgcttaac gtttgggcta cctggtgtcc gacctgccgt gcggaacatc
    27061 aatatctgaa tcagctttct gcgcagggca ttcgcgtggt cggcatgaac tataaagacg
    27121 atcgccagaa ggcaataagc tggctgaaag agctgggcaa tccttacgcg ctaagcctgt
    27181 ttgatggcga tggcatgtta gggctggatc tcggtgtcta tggcgcgcca gaaacgttcc
    27241 tcatcgacgg caacggcatc attcgctatc gccatgcggg cgatctcaat cctcgcgtct
    27301 gggaagaaga gatcaagccg ctgtgggaga aatacagtaa ggaggccgca caatgaggtt
    27361 tttattgggc gtgctgatgc tgatgatctc cggctcagcg ctggcgacca tcgacgtgtt
    27421 gcagtttaag gatgaagcgc aggagcagca gttccgccaa ctcactgaag aactacgctg
    27481 cccgaaatgc cagaacaaca gcattgccga ttccaactcg atgattgcca ccgacctgcg
    27541 ccagaaagtg tatgaactga tgcaggaagg taaaagtaaa aaagagattg tcgattatat
    27601 ggtggcgcgt tacggcaact tcgtcactta cgatccgccg ttaacgccgc tgaccgtgct
    27661 gctgtgggtg cttccggtag tggctattgg cattggcggt tgggtcattt acgcccgttc
    27721 gcggcgtcgg gtacgcgtgg tgccggacgc gtttcctgaa caaagcgtgc cggaaggtaa
    27781 gcgtgccgga tatattgttt atctgccggg tattgtggtg gcgttaattg tggctggcgt
    27841 cagctactac cagactggca attatcagca ggtgaaaatc tggcagcagg ccacggcaca
    27901 ggctccggcg ttgctggaca gggcgctgga tccgaaagcc gatccgctca acgaagaaga
    27961 gatgtcgcgt cttgcgctgg ggatgcgtac tcaactgcaa aaaaatccgg gagatataga
    28021 aggctggatt atgttgggcc gcgttggcat ggcgctgggt aacgccagta tcgccaccga
    28081 tgcatacgct actgcgtatc gcctcgatcc gaaaaacagt gatgccgcac tgggttatgc
    28141 tgaagcgttg acacgttcat ctgatcccaa cgacaaccgc ctcggtggtg aactgctgcg
    28201 ccagttggtg agaacggacc atagcaatat ccgtgtgtta agcatgtatg cgtttaatgc
    28261 ctttgagcag cagcgatttg gcgaagccgt tgccgcgtgg gagatgatgt tgaaactctt
    28321 atctgccaac gatactcgcc gtgcggtgat tgaacgtagt atcgcgcagg cgatgcaaca
    28381 tttgtcgccg caggagagta aataaaaaga ggcccgatgg tgatgccatc gggctatttt
    28441 tattgtgccc cgcgttgttg caggaacaga atggtggctg ccacgcgtga gcggacattg
    28501 agtttgcgca gcagattgcg aatatgtact tttactgtct gctctgaaat attcaacacc
    28561 gaggcaatct gtttatttga cagcccctgt gccagctcgt gcagaacatc cagctcgcgc
    28621 tccgtcagca cgctgaaggg atcttcttcc gcgccaaaca tttcacgttc acgtaagtac
    28681 tgattgacgc gttcgctaaa gactttgctg cctttcgctc cagcacgaat tgcttccagc
    28741 aatacttccg ggtcgctgtc tttcaacaga taaccgtctg cgcctgcgtc tatcagcgca
    28801 aagacatcgc tggaggcatc tgatacggtc aggataataa tttgcgcggt aacgccatcc
    28861 ctgcgcaagg cattgagggt atccaggcca ctcatacctt tcatattgag atccagcaag
    28921 atcacgtcga tatccagtct atttgccaga tcgatcgcgc tcgcgccgtc gcccgcttcg
    28981 gcgaccactt caaagctagg atcaagctcc agtaactgac gaacaccgcg tcgcataagt
    29041 ggatgatcat ccacaatcat cacctgaaaa ggcgttgctt caggcatagt agtctcctgg
    29101 ggttttatta gaatcattat gtttgagcgt agcgttggta acgctaccag gcgcgtgaat
    29161 gttaccccat tttatgtatg gacatgtagt gaaaactaca accttgtcgt tcgctttggc
    29221 atcattttca ttataaagac ggaattttgc atcagcttct c
//
LOCUS       DAJTSL010000034        29039 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTSL010000034 DAJTSL010000000
VERSION     DAJTSL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.034"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            28..1347
                     /gene="potE"
                     /locus_tag="OO433_001357"
     CDS             28..1347
                     /gene="potE"
                     /locus_tag="OO433_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6049899.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPCFELKNKHG"
     gene            1423..1572
                     /locus_tag="OO433_001358"
                     /pseudo
     CDS             1423..1572
                     /locus_tag="OO433_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            1696..2346
                     /locus_tag="OO433_001359"
     CDS             1696..2346
                     /locus_tag="OO433_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HCR6049900.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKNLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(2387..2881)
                     /locus_tag="OO433_001360"
     CDS             complement(2387..2881)
                     /locus_tag="OO433_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049901.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     RLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(3096..4736)
                     /gene="pgm"
                     /locus_tag="OO433_001361"
     CDS             complement(3096..4736)
                     /gene="pgm"
                     /locus_tag="OO433_001361"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6049902.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMEAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(4762..5307)
                     /gene="seqA"
                     /locus_tag="OO433_001362"
     CDS             complement(4762..5307)
                     /gene="seqA"
                     /locus_tag="OO433_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6049903.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5492..6256
                     /gene="ybfF"
                     /locus_tag="OO433_001363"
     CDS             5492..6256
                     /gene="ybfF"
                     /locus_tag="OO433_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6049904.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPSGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6396..6689
                     /gene="ybfE"
                     /locus_tag="OO433_001364"
     CDS             6396..6689
                     /gene="ybfE"
                     /locus_tag="OO433_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6049905.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            6829..7359
                     /gene="fldA"
                     /locus_tag="OO433_001365"
     CDS             6829..7359
                     /gene="fldA"
                     /locus_tag="OO433_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6049906.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7569..7655
                     /gene="uof"
                     /locus_tag="OO433_001366"
     CDS             7569..7655
                     /gene="uof"
                     /locus_tag="OO433_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6049907.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7648..8094
                     /gene="fur"
                     /locus_tag="OO433_001367"
     CDS             7648..8094
                     /gene="fur"
                     /locus_tag="OO433_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6049908.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8179..8505)
                     /locus_tag="OO433_001368"
     CDS             complement(8179..8505)
                     /locus_tag="OO433_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6049909.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFPDQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8555..9961)
                     /gene="chiP"
                     /locus_tag="OO433_001369"
     CDS             complement(8555..9961)
                     /gene="chiP"
                     /locus_tag="OO433_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6049910.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPAYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10408..12072)
                     /gene="glnS"
                     /locus_tag="OO433_001370"
     CDS             complement(10408..12072)
                     /gene="glnS"
                     /locus_tag="OO433_001370"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6049911.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTRQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSSEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12276..14222)
                     /gene="nagE"
                     /locus_tag="OO433_001371"
     CDS             complement(12276..14222)
                     /gene="nagE"
                     /locus_tag="OO433_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6049912.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPSTAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14555..15355
                     /gene="nagB"
                     /locus_tag="OO433_001372"
     CDS             14555..15355
                     /gene="nagB"
                     /locus_tag="OO433_001372"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6049913.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15415..16563
                     /gene="nagA"
                     /locus_tag="OO433_001373"
     CDS             15415..16563
                     /gene="nagA"
                     /locus_tag="OO433_001373"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6049914.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            16572..17792
                     /gene="nagC"
                     /locus_tag="OO433_001374"
                     /gene_synonym="nagR"
     CDS             16572..17792
                     /gene="nagC"
                     /locus_tag="OO433_001374"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6049915.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYIGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            17840..18592
                     /gene="nagD"
                     /locus_tag="OO433_001375"
     CDS             17840..18592
                     /gene="nagD"
                     /locus_tag="OO433_001375"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR6049916.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETSSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            18989..20653
                     /gene="asnB"
                     /locus_tag="OO433_001376"
     CDS             18989..20653
                     /gene="asnB"
                     /locus_tag="OO433_001376"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6049917.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21034..21110
                     /locus_tag="OO433_001377"
     tRNA            21034..21110
                     /locus_tag="OO433_001377"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21068..21070,aa:Met,seq:cat)
     gene            21119..21203
                     /locus_tag="OO433_001378"
     tRNA            21119..21203
                     /locus_tag="OO433_001378"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21153..21155,aa:Leu,seq:tag)
     gene            21227..21301
                     /locus_tag="OO433_001379"
     tRNA            21227..21301
                     /locus_tag="OO433_001379"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21259..21261,aa:Gln,seq:ttg)
     gene            21336..21410
                     /locus_tag="OO433_001380"
     tRNA            21336..21410
                     /locus_tag="OO433_001380"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21368..21370,aa:Gln,seq:ttg)
     gene            21426..21502
                     /locus_tag="OO433_001381"
     tRNA            21426..21502
                     /locus_tag="OO433_001381"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21460..21462,aa:Met,seq:cat)
     gene            21551..21625
                     /locus_tag="OO433_001382"
     tRNA            21551..21625
                     /locus_tag="OO433_001382"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21583..21585,aa:Gln,seq:ctg)
     gene            21663..21737
                     /locus_tag="OO433_001383"
     tRNA            21663..21737
                     /locus_tag="OO433_001383"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21695..21697,aa:Gln,seq:ctg)
     gene            complement(21891..23066)
                     /gene="ubiF"
                     /locus_tag="OO433_001384"
     CDS             complement(21891..23066)
                     /gene="ubiF"
                     /locus_tag="OO433_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6049918.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23212..24636
                     /gene="miaB"
                     /locus_tag="OO433_001385"
     CDS             23212..24636
                     /gene="miaB"
                     /locus_tag="OO433_001385"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6049919.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPSETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            24789..25829
                     /locus_tag="OO433_001386"
     CDS             24789..25829
                     /locus_tag="OO433_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6049920.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLTIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            25826..26293
                     /gene="ybeY"
                     /locus_tag="OO433_001387"
     CDS             25826..26293
                     /gene="ybeY"
                     /locus_tag="OO433_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6049921.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26383..27261
                     /gene="corC"
                     /locus_tag="OO433_001388"
     CDS             26383..27261
                     /gene="corC"
                     /locus_tag="OO433_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6049922.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27286..28824
                     /gene="lnt"
                     /locus_tag="OO433_001389"
     CDS             27286..28824
                     /gene="lnt"
                     /locus_tag="OO433_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6049923.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTHEVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
BASE COUNT         7092 a         7442 c         7397 g         7108 t
ORIGIN      
        1 aagcaccctg ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc
       61 gttcagttaa ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg
      121 acaaagcttg ccgaagttgg gacaatctca attatctcct ggctggtgac agccgtaggc
      181 tcaatggcac tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggcggt
      241 atgggcggtt atgcggagta tgctttcggt aagtccggca attttatggc gaactacacc
      301 tacggtgtct cgctgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc
      361 accgaactgc tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggagtg
      421 ctgtggattt gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc
      481 attactgtgt ggggagtcat tattccggtc gtcggtctgt gcattattgg ctggttctgg
      541 tttagtccga cgctgtacgc taactcatgg aacccgcacc atgtgccgtt ctttagcgct
      601 gtaggttctt ccatcgccat gacgttgtgg gctttcctcg gtctggagtc agcctgtgcc
      661 aacgccgaag tagtggaaaa cccggaacgt aacgtgccga ttgcagttct tggcggtact
      721 ttaggtgcgg cggtgattta tatcgtctcc accaacgtga ttgccgggat tgtgcctaat
      781 atggagctgg caaattcaac ggcaccattt ggcctggctt tcgcacagat gttcacacca
      841 gaagtgggta aagtcattat ggcgctgatg gtgatgtcct gctgcggttc actgcttggc
      901 tggcagttca ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa
      961 attttctccc gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt
     1021 attcagagtg gattggcact gatgaccatt agcccgtcac tgaacagcca gttcaacgtg
     1081 ctggttaacc tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta
     1141 gtcattattc agaaggtcgc taatgtgccc ccctcaaaag cgaaagtagc aaactttgtt
     1201 gcctttgttg gtgcgatgta cagtttctat gcgctgtact catccgggga agaagccatg
     1261 ctgtacggtt ctatcgtgac cttccttggt tggacactgt atggtctggt atcaccatgc
     1321 tttgaactga aaaataaaca cggttaataa aacaaagtaa aaaagggcga ttgaatgatc
     1381 gccctttctg tttgttggca aatagttcgt tatttatgcc gcatccggca gatgtagggt
     1441 ctaaacgcga tcgaacacag caactatttg cgtaattata tggggcatct gcgacaaaaa
     1501 ctggaacagg atccggcccg gcctcgccat tttattactg aaaccggtat tgggtatcgg
     1561 tttatgcttt gaaaaaagtg gcaatgtatg gataaagtca ttttttattc aaatataagg
     1621 aacttaatat ttaaaaaatg ttccatacaa ttcctcatca taaatgacac cgtagcacac
     1681 aaggagcaag tagcgatgcg tgaaatgaat cagttagtaa atactgaaga ttcagcctgg
     1741 ccaataatcc agaactggtt aaaagacgcc acaaaccata ccgaactctt accggttaat
     1801 aaaaatttag ccgaaaccgc actttatcaa ttacaggtga caacaaaatc accgatgggt
     1861 gcgcttgtct atggctccgg aggtctgctg attgataatg gttggttacg catagcagga
     1921 tctggtcacc cccggttgcc acgagatcca gtaagttgga cacaacgccc tgaattcgca
     1981 ggcgtgcggg ctttacccat cgctgacgat gtcgcagggg gaatttttgc tcttaatggt
     2041 ggcgatcttg gggaagatac tggctgcgtt tattattttg cgccagatac gctgaattgg
     2101 gaaagtctgg aagttggata ttctgagttt cttcagtggg cattatccgg tgatttagat
     2161 accttctatg aaaatgtacg atggcagcaa tggcgagaag acgtaataaa attatcggca
     2221 acggaagcct tcacgttcta ccctttctta tgggtacaaa gtgaagaagc aagaacaaga
     2281 aaagttattt cgcttactga actctgggaa atgcagtatc aaatgaaaga aacattcacg
     2341 caataagaca tgttcaggtc cgggtttcat tcccggactc actattttaa tgtgtattta
     2401 tcgctttcaa ttcttgctgg caattttctg actggcagcg tcgtaactca ataatcccga
     2461 catgcatcat cgagccaaaa aatccgcttt cgctgacata atcctcattg actatcggtc
     2521 gagtgccggg cttgccctgg caaacccgca taaaaaatga ggtgtagcat tgccaaccgg
     2581 gatttaaacc gaccttttct tgtgctgaca tattatgaag ataagtaagt tctttttcgt
     2641 tcccgcctga caactgccag cggcgggtat tatcaggtaa gttatcacca cggcaatttg
     2701 tcttacaccg ggaaggtgca atatttaccg caatgctacc cgttaatctt ctggttttat
     2761 ctttaggggt cagtaaccag tcgccagcta atgatgattg ttcaggttgg gcgcatgcag
     2821 tcagcagtaa tgtggcggta attagtgaca ctatgacgac cagactcctg ttggtcttca
     2881 ttgggatttc cttgagctac tcagtttgtt gacaaagtgt tcgttgtttt tgccggatgc
     2941 ggcgtgaacg ccttatccgg cctacaaaat ctcgtaaatt caatatattg caccatcctc
     3001 gtaggcctga taagcgtagc gcatcaggca attctgtgtt tgtcatacgt aaaaaagggc
     3061 gatcttccga ccgccctttt tcttatttaa tgtgtttacg cgtttttcag aacttcgcta
     3121 acaatctcaa ccgcttcttt ctcaatctgc ttgcgatgtt cttcaccgag gaagctttcg
     3181 cagtagatct tatatgcgtc ttccgtgcct gacggacgcg cggcgaacca gccgttgtca
     3241 gtcatcactt tcagaccgcc gatagaagca ccattgcccg gtgccgccgt caggcgcgcg
     3301 gtgatcgggt cgcctgccag ggtgctggcg ctcaccattt ccggagacag cttagacagc
     3361 gccgcttttt gtgcggaagt cgcagctgcc tgcaaacggt tgtagctcgg cgcaccaaag
     3421 cgttttgcca gttcgttgta gtgttcctgc gggttcttac cggtgacagc ggtgatttcc
     3481 gccgccagca gacacatgat gatgccgtct ttgtcggtgg accacggcgt gccgtcgaaa
     3541 cgcaggaagg aagcccctgc gctctcttcg ccgccaaagc cgaagctgcc gtcgaacaga
     3601 ccatcaacaa accatttgaa acctaccggg acttctacca gcttgcggcc caagtcattg
     3661 accacacggt cgatcatcgc agaagaaacc agcgttttac cgacagcaac atctttgccc
     3721 cactgcggac gatgctggaa caggtagttg atcgccaccg ccaggtagtg gttcggattc
     3781 atcaaacctg ccggagtgac gataccgtga cggtcataat ccgggtcgtt agcaaacgcc
     3841 agatcgaact tatcacgcag tgccagcagg cccgccatcg cacactcgga ggagcagtcc
     3901 atacggatcg cgccgtcttt atcaaggtgc ataaagcgga aggtttgatc gacctgatcg
     3961 ttaacgatgg tcaggttgag gttgtaatac tcgccaatac gcttccagta ttcgataccg
     4021 gaaccgccca gcggatcaac gcccagcgtc aggcccgctt tctgaatcgc ctccatatca
     4081 acgatatcgg ccagaccttc cacaaacggc tgcaccagat cctgctcttt cacatgaccg
     4141 gatgccatcg cttcgtcgag ggagatacgc ttcacgcctt tcaggccatc ggccagcagt
     4201 gcgttggccc tgtcttccac cactttggtg acgttggtat cagccgggcc accatttggc
     4261 ggattgtact tgataccacc atcttccggc gggttatggg acggtgtaat cacgataccg
     4321 tctgccagcg gaccaccttt tttattgtga accaggatgg cattggaaac ggcaggcgtt
     4381 ggagtgaagc cattgttttc ctgcacaatg acatcaacgc cgttcgctgc cagcacttcc
     4441 agtacggaaa tgaacgcagg ctcggacagg gcgtgagtat ctttacccac atagcaaggg
     4501 ccagtgatgc cgtttttcgc acgttcttca gcaattgcct gagcgatcgc cagaatgtgc
     4561 ggctcgttaa agctgtggcg cgctgcactg ccacggtgac cggaagtacc gaatttcacc
     4621 gcgtgctccg cattccctgc ttctggtttc agtacataat attgcgccgt cagttgggcg
     4681 acgttaatca aatcactctg ttgtgcaggt tggcctgcac gattgtggat tgccattgct
     4741 ttgtcctttg tctgcaacgt tttagatagt tccgcaaacc ttctcaatca attccgccgg
     4801 gaattgcatc gactgcatga tgtgttcgat catgctgcat ttacggccgg tgttggtgtt
     4861 ggtgatcacc caatacggcg tgcctggcac atgtttcggc ttggtctgat taccattttt
     4921 cagcagcgtt tgttcatctg ccgcaaagta aacgcgtgta cgaccgtgca acgattccgt
     4981 tgcttcggca aacgcctggg cgtcaagaga atatagtgta gacaacagca gcataaagcg
     5041 attgaccgct cgcttttgct ctgcgtattc gtcagaaagc agaagttcac gcattgcgcg
     5101 aaccttgtct ttaatcgttt tgaccggctt cgcttcgacg atagcaggtg acgcaacgcg
     5161 aacctctttc gtcaccggag cagcaggctg tgatgcggcg gaaaatttca acatacgccg
     5221 taaaatgtcg gatgcgctct cgccgatatg cttagtgtgg ctggcaatat agctgtagag
     5281 ttcatcatca acttcaatcg ttttcatctt aatccagtgc agtgtcttag ctgaatacaa
     5341 gtcgccaaga gtatatgaat aaatcccggc agcggatagc gtcaagcctg gcttgcagaa
     5401 aaactcggaa taaaccttat ttgttgcagc cctgtggcac aatggtcaac ataataccct
     5461 aacccgacaa cgcgaaaaaa agaactttgc catgaaattg aatatccgcg cgcaaactgc
     5521 acaaaaccag cacaataatt ctcccatcgt tcttgtccat ggtctgtttg gtagcctcga
     5581 caaccttggc gtactggctc gcgatctggt aaacgatcac aatatcatcc aggttgatat
     5641 gcgtaaccac ggtctttcac cgagagatcc ggtaatgaat tacccggcga tggcgcagga
     5701 tcttgttgat actctggatg cacagcagat cgacaaagca acatttatcg gtcactccat
     5761 gggcggtaaa gcggtaatgg cacttacggc actggcccgc gatcgcatcg ataaactggt
     5821 ggcgatcgat atcgcaccgg tcgactatca cgtacgccgt catgatgaga ttttcgcggc
     5881 tatcaacgcg gtcagtgaat cggacgcaca aactcgccag caagcagcag caataatgcg
     5941 ccagcatctt aatgaagaag gggtgattca gtttctgttg aaatcttttg ttgacgggga
     6001 gtggcgcttt aacgtgccgg tattgtggga tcagtatccg catattgtgg gttgggagaa
     6061 aatcccggca tgggatcacc ctgccctgtt tatccctagc ggcaattctc cgtatgttag
     6121 cgagcagtac cgtgatgatt tactggcaca atttccacag gcacgggcgc atgtgattgc
     6181 aggcgcgggt cactgggtcc atgctgaaaa accggatgcg gtattacgcg ctatccgtcg
     6241 ctatctcaat gattaacgca actgattaaa aacccagcgt ccgcgtcgct ttccgcgcgt
     6301 gggcgttggc gtgactcatc gcgctgatgt attatggcgc gctatccatc cgggcagagg
     6361 cctggctgat tgtttccccc gaagtcacca agatcatggc caaagaacaa acggaccgta
     6421 cgacattaga tctgttcgcg cacgagcgtc gaccgggacg accgaaaact aatccgcttt
     6481 cgcgcgatga acagctgcgt attaataaac gcaaccagct aaaacgcgac aaagtacgtg
     6541 gccttaagcg tgtcgaactg aagctgaacg cggaagctgt cgaggcgctg aacgagctgg
     6601 cggagtcgcg caatatgagc cgtagcgaac tgatcgaaga gatgttaatg cagcaactgg
     6661 cggcgctgcg tagtcagggc atcgtttaaa tttccacttt catgtagcac agtgtgcagt
     6721 cctgctcgtt tgctgattcc gaaagactgc ctgttctgct atgattgcct ttatccgtgg
     6781 gcaattttcc acccccattt caataagttt caagaggtta tttcactcat ggctatcact
     6841 ggcatctttt tcggcagcga caccggtaat accgaaaata tcgcaaaaat gattcaaaaa
     6901 cagcttggta aagacgttgc cgatgtccat gacattgcaa aaagcagcaa agaagatctg
     6961 gaagcttatg acattctgct gctgggcatc ccaacctggt attatggcga agcgcagtgt
     7021 gactgggatg acttcttccc gactctcgaa gagattgatt tcaacggcaa actggttgcg
     7081 ctgtttggtt gtggtgacca ggaagattac gccgaatatt tctgcgacgc attgggcacc
     7141 atccgcgaca tcattgaacc gcgcggtgca acgatcgttg gtcactggcc aactgcgggc
     7201 tatcatttcg aagcatcaaa aggtctggct gatgacgacc actttgtcgg tctggctatc
     7261 gacgaagacc gtcagccgga actgaccgct gaacgtgtag aaaaatgggt taaacagatt
     7321 tctgaagagt tgcatctcga cgaaattctc aatgcctgat gtgatgcggc gtagactcat
     7381 gtctacgccg tattaataga taatgccaat caaaataatt gctacaaatt tgtaactttt
     7441 gctgttgtac ctgtacaatg tcccggtgtt caagtggcct tgccgttgta aatgtaagct
     7501 gtgccacgtt tttattaaca atatttgcca gggacttgtg gttttcattt aggcgtggca
     7561 attctataat gatacgcatt atctcaagag caaattctgt cacttcttct aatgaagtga
     7621 accgcttagt aacaggacag attccgcatg actgataaca ataccgccct aaagaaagct
     7681 ggcctgaaag taacgcttcc tcgtttaaaa atcctggaag ttcttcagga gccggacaac
     7741 catcacgtca gtgcggaaga tttatacaaa cgtctgatcg atatgggtga agaaattggt
     7801 ctggctacgg tatatcgcgt actgaaccag tttgacgacg ctggtatcgt cacccgccac
     7861 aattttgaag gcggtaaatc cgtatttgaa ctgacacagc aacatcacca cgatcacctg
     7921 atctgcctcg actgcggcaa ggttatcgaa tttagtgatg attccatcga agcgcgtcag
     7981 cgtgaaattg ccgcaaaaca tggcattcgc ctgactaacc acagtctcta tctttacggt
     8041 cactgtgccg aaggcgattg ccgcgaagat gaacatgcgc acgaaggcaa ataagccagc
     8101 ctgaatgaga aaaagccaac ctgcgggttg gcttttttat gcaagggaaa taagaaaagc
     8161 gagagttaca gctctcactt atttgttatt actgcgaatt tcctgccaaa ctttatcaca
     8221 atcggctttc accgcctgat catttccggt ttgcgttgca cgcagacact gctgataatc
     8281 cagtacgcgg acactttcct ggtcgggaaa atgctgatgt tgtttctctt tcttcagcac
     8341 gtttaacacg ctctggcagg cttcaatttt ttctggcgaa ccttgtgcgg tgttgataca
     8401 ggcgctgtat gcctctttca gacgactgtc ttcctgtggc gcggttgatt gcgcacaagc
     8461 taccagcccc gatgccatta tggcgatgag aatcagtttt ttcatagcaa attcctcaaa
     8521 cgtgcggcag gcacaaaacc tgccgcgtcg ggcatcagaa gatggtgaat ggtgcgatta
     8581 ccataaattt cacgtcacgc tcatcctgga agatgttgcc gtaaccaccg ccccagcttg
     8641 ggatgtcgga gtggttgtcg tattcggtga agtgcagttt gaacatcgtg cctttggcgc
     8701 gaccgtcctg aatggtgtag accgcatcca ggctgtatgc agactcttca atagtccggt
     8761 ttttgtcgta gtacgcatcc ggattgctct gccaggtcgc aggtttagca tcccatgcgt
     8821 aaacgtagga agcgccgatg gcgaagcctg gaagattcca gtttttcagg tcatacatcg
     8881 caccgaagaa gaccgctttt tcgccgttag cgttgaagtc ggaacggtta tcccaccaga
     8941 tatccaggcg gccgttggag gaagcgtagg ctggagtcat acgttgcagg aagtatccct
     9001 gctgaccgtc agccttaacc caggtgcctt cgaggcgcaa atccactacg tcagccgccc
     9061 ggtaaccaaa ggtcaacgct tgcagccagg cggtgccgtc ataaaggtcg ttgacgctgc
     9121 gatcgtcaac tttatcgcgc gtaccataga actggtagct ggtggttaac gggctaccgg
     9181 cgatatcaaa tttgtagctg gctttggcaa aatattgatc gatataccct tccgcctgac
     9241 caaatgccgc ttccagtacg aagttatttt tgaagtcgta tttcgcccca agggagtgca
     9301 gataatcaac tttggtggtt ttatcgttct gataaaactc atccatttcc agatgccacg
     9361 gcgctttgta ttcgttggtc cacatgtagg agaaactcaa cgcaccagca tcgccgtaat
     9421 caaaattcgc cccggcttcc gccccctgat aagtacctgg cataaagctc cagtgcggcg
     9481 ctaacagcgt ttgaccagtt ggctgaatgt aacctgccct cgcccaaacc ggaccgtatt
     9541 taaatttggc cgcagcttta tacaggctta taccgctttt gtcgccagac cagtcttcgt
     9601 cataggcttt attacttttt gaaaacgcga tttcgttcgg gtgggagctg tcgccgtttt
     9661 ccgccatttc aatcgccgta aacacagcaa tatcaaggcc gaacatatca gcagcataac
     9721 cggactgaaa atcgaggttg gcattccagg tggagtgaga aaggttggtt ttgtatttgt
     9781 cgccgtcggt aacatcttta cggtcgcgtt cacgctgcca gtaatatata ccgccggtta
     9841 aggttgaatc gtcgatgaat ccttcggcgc gagcctccgg cattgccata aagcccgaca
     9901 ttgctgtaac accggcgata gccagcgcca gcgtactacg tttgccacta aacgtacgca
     9961 tgggttaatc ctctttgacg tataaattgc tgcaccaaag gtgaacagcg aaaaaaataa
    10021 aaattaaaaa agtagcattg agaaaagtct cgctgactac aggaatagac tatttttcgc
    10081 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    10141 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa
    10201 agcgtaaatt tccagcaaga tgaaagcggt tacattttat ttaattatgg tttgtgattt
    10261 taaatttatt ttatggataa attgcgaagc ggaattaatt cgcgaagaaa agcagaaaaa
    10321 acgccgcaga gtgcggcgtt tgggattgtt tgctatttct tgccggatgc gacgtaaatg
    10381 cctcacccgg cctggtagaa cttaaaatta ctcgcctact ttcgcccagg tatcacgcag
    10441 cccaacggtg cggttaaata ccggtttttc cgctgtggaa tggcggctgt cgaggcagaa
    10501 gtaaccttca cgctcaaact ggaatgcttt acccgcaacc gcatctttca gcgacggttc
    10561 agcaaagccc tgtttgatca ccagcgattc cgggttaatc accgacagga aatcatccgc
    10621 agcacctggg ttaggcacgc tgaacagacg gtcgtacaga cggatttcaa ccggcagcgc
    10681 atgtgccgcg ctcacccagt gaataacacc tttgacttta cgaccatctg ccggatcttt
    10741 gcttaaggtg tcggcgtcat aagtacagaa gatggtggtg atattgcctt cggcatcttt
    10801 ctccacgcgt tctgccttaa tgacgtaagc attacgcaga cgcacttctt tacccagcac
    10861 cagacgtttg tactgcttgt tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc
    10921 actgctaaac ggcacctggc ggctacccat ttccggtttg ttcggatggt tcggcatggt
    10981 aaccatttcg ccttcgccct gatagttttc gataaccagt ttcaccggat cgataaccgc
    11041 cattgcgcgc ggggcgtttt cgttgagatc ttcacggatg caggattcca gcgacgccat
    11101 ctcaatggtg ttgtcctgcc tggtcacgcc gatgcgtttg cagaactcac gaatagaagc
    11161 agcagtgtaa ccacgacgac gcagaccgga aatggtcggc atacgcgggt catcccagcc
    11221 ttcaacgtgc ttgtcggtca ccagcaggtt cagcttacgc ttggacatca cagtgtattc
    11281 cagattcagg cgcgagaatt cgtactggcg cgggtgaaca ggaatggtga tgttgtccag
    11341 cacccagtcg tacaggcgac ggttgtcctg gaactcaagc gtacacagag agtgcgtaat
    11401 accttccagg gcatcgctga tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt
    11461 gttgccagtc tggtggtgtt cagcaaattt aatgcggtac agcaccggat cgcgcatcac
    11521 gataaacggt gaagccatgt cgattttcgc acgcaggcag gctttacctt cttcaaaacc
    11581 accggcacgc attttttcga acagcgccag gttctcttca acgctgcggt cgcggtacgg
    11641 gctgttttta cccggttgcg tcagggtgcc gcggtattcg cggatctgtt ccggcgtcag
    11701 ttcatcaacg tacgccaggc ctttattgat cagttcgatc gcataggcgt ggagctgatc
    11761 aaaataatcg gaggagtaac ggacgttacc agaccagtga aaacctaacc actctacgtc
    11821 gtttttgatc gactcaacat actcgatatc ttcttttacc gggttagtgt cgtcgaaacg
    11881 caggttgcac tggcctttat agtcctgggc gatcccgaag ttcaggcaga tagatttcgc
    11941 atggccaata tgcagatagc cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg
    12001 cttaccactg gccagatctt catcgatgat ctgacggata aagttagtcg ggcgggcttc
    12061 tgcctcactc atcgtggatt cctcaaagcg taaacaacgt ataacggcgt atgatcttat
    12121 aagcggaaca ggctgacaac tgttagtttt ttaaaattga taatctaaca aggattatgg
    12181 ggatgattga tgaaaaaaaa aagcggcaga gaagatctcc accgcttgtc attgttggat
    12241 gcgacgctca agcgtcgcat caggcataaa gcagattact ttttgatttc atacagcggt
    12301 gtttgacccg ccacaacatg gccctgagct ttaatgatca agccactgaa atcgtcgata
    12361 ttgctgcaaa ccaccgggct aatcatcgag cgggcgttag cgttcaggta atccagatcc
    12421 atttccagaa tcggttgccc tgcgcttacc tgcgcaccct cttccaccag acgtttaaag
    12481 cctttacctt ccagcgctac ggtgtcgata cccatatgga cgacgatctc cgcgcctttt
    12541 tcggtttcca ggcagaacgc gtggttggtg ttgaagattt tcacgattgt ccctgcggct
    12601 ggtgatacga cgattttatc tgtcggtttc accgccacac cgtcacccac cgctttgctg
    12661 gcgaatgctt cgtcaggaac ctgatccagt gccacgacat caccggtaat cggcgatacc
    12721 agctccgcga tagataccgc gtttggtaca gcctgcggtt ttgctacagg cgcggcagtt
    12781 gacggagttg cttcagctga cgcagcggct accggaccac gggcaacgac tttcttcatc
    12841 gcatcgccga tggattctgc tttcgcgcca acaatcacct gaatagtctg tttgttcagt
    12901 ttcactacac cagaagcacc cagacgttta cacatcgtat cgttgacgcg ggcagagtca
    12961 gccacggtca ggcgcagacg ggtgatacag gcgtcaattg ctttcaggtt gtcagtgccg
    13021 ccaaccgcag caatatagtt ggtcgccagt tgattgaggc cttcttcagt gttgctgttg
    13081 gcttcttcag taacgatctc atcttcttta tcttcacgac ccggcgtttt caggttgaac
    13141 atgcggataa ccaaactgaa caccacgaag tagatagcga agaagacaac acccatcacc
    13201 agcagcatcc agacgttctg gctggcggcc ggcaggttat acatcaacgc gtagtcgata
    13261 gcccccgcag agaaagagaa gccagcatgg atacccagca gtgttgccac aaacaggctg
    13321 ataccggtca gcagtgcgtg caggaggtac agcagcggtg caaggaacat gaacaggaat
    13381 tccagcggct cagtcacacc ggtcaggaac gcagtaacag caacagaaag cagcatcccg
    13441 ccaaccatcg gacgacgctc tttcggtgct gcgaagtaca tcgccagcgc cgcacccggc
    13501 agaccgaaca tcataatcgg gaagaagccg gacatgaaca tccccgcggt gccgtcaccg
    13561 gcatagaagc ggttgatatc accgtggaaa accgtacccg ccgcgttggt gaattcacca
    13621 atctggaacc aggcgatggt gttcagcacc tgatgcagac cggttgggat cagcagacgg
    13681 ttgatgaaac caaagatacc ggaacccagc gcgcccgcag aaacgatcca ctcgcctcct
    13741 gcatggatag cgtgctgtac cggcggccag acgtaaccaa aaatggccgc cagcaccagg
    13801 cagaagaagc cggtggcaat cggcacaaag cgtttgccgc cgaagaagct caggaagtcc
    13861 ggcagtttaa tatcggacca acggttatag gctgcgccac caaccagacc ggtaatgata
    13921 cccgccagta cacccatgtt tatttctggg ttgatggtca ccatcgcttt ggttaacaca
    13981 aagtaaccta ccgcacccgc cagcgccgcc gcacctgcgc tgtctttcga ccagctggat
    14041 gccacaccga tggcgaagat taatgcgagg ttatcaaaaa tcgcaccgcc cgcctgggca
    14101 ataaacgcaa cgttaagtaa atctggctga ccgaatcgca gcaacagtgc cgccaccggc
    14161 agcaccgcga tagggagctg taacgcccta ccgagtcgct ggaaaaaacc taaaatattc
    14221 atcttattcc ccctacgaga accctatttg gctcgtttaa agccgtattt ttattttgct
    14281 gcaaattgta ctgccgatgt tctgtaatca gattgttaga tcatctgcta cagagtgtgt
    14341 gaaaatttaa ttcgtatcgc aaattaaacg cgtgtctttt gtgagttttg tcaccaaata
    14401 tcgttatcat cactcccttt tactggctaa accagaaaac ttattttatc attcaaaaaa
    14461 tcaggtcgga ttgacgcctg tctgcgcaaa tccaagttac gcttaaagat gcctaatccg
    14521 ccaacggctt acattttact tattgaggtg aataatgaga ctgatccccc tgactaccgc
    14581 tgaacaggtc ggcaaatggg ctgctcgcca tatcgtcaat cgtatcaatg cgttcaaacc
    14641 gactgccgat cgtccgtttg ttctgggcct gccaactggc ggcacgccga tgaccaccta
    14701 taaagcgtta gtcgaaatgc ataaagcagg ccaggtcagc tttaagcacg ttgtcacctt
    14761 caacatggac gaatatgtcg gtctgccgaa agagcatccg gaaagctact acagctttat
    14821 gcaccgtaat ttcttcgatc acgttgatat tccagcagaa aacatcaacc ttctcaacgg
    14881 caacgccccg gatatcgacg ccgaatgccg ccagtatgaa gaaaaaatcc gttcttacgg
    14941 aaaaattcat ctgtttatgg gcggtgtagg taacgacggc catattgcat ttaacgaacc
    15001 ggcgtcttct ctggcttctc gtactcgtat caaaaccctg actcatgaca ctcgcgtcgc
    15061 gaactctcgt ttctttgata acgatgtcaa tcaggtgcca aaatatgccc tgactgtcgg
    15121 tgttggtaca ttgctggatg ccgaagaagt gatgattctg gtgctgggta gccagaaagc
    15181 actggcactg caggctgcag ttgaaggttg cgtgaaccac atgtggacca tcagctgtct
    15241 gcaactgcat ccaaaagcga tcatggtgtg cgatgaacct tccaccatgg agctgaaagt
    15301 taagacttta agatatttca atgaattaga agcagaaaat atcaaaggtc tgtaattgtt
    15361 atccctgccc tggctccttg ctcagggcaa tattttttaa aatcgggggt cagaatgtat
    15421 gcattaaccc agggccggat ctttaccggc cacgaatttc ttgatgacca cgcggttgtt
    15481 atcgctgatg gcctgattaa aagcgtctgt ccggtagcgg aactgccgcc agagatcgaa
    15541 caacgttcac tgaacggggc cattctctcc cccggtttta tcgatgtgca gttaaacggc
    15601 tgcggcggcg tacagtttaa cgacaccgct gaagcggtca gcgtggaaac gctggaaatc
    15661 atgcagaaag ccaatgagaa atcaggctgt actaactatc tgccgacgct tatcaccacc
    15721 agcgatgagc tgatgaaaca gggcgtgcgc gttatgcgcg agtacctggc aagacatccg
    15781 aatcaggcgt taggtctgca tctggaaggt ccgtggctga atctggtaaa aaaaggcacc
    15841 cataatccga attttgtgcg taagcctgat gccgcgctgg tcgatttcct gtgtgaaaac
    15901 gccgacgtca ttaccaaagt gaccctggca ccggaaatgg ttcctgcgga agtcatcagc
    15961 aaactggcaa atgccgggat tgtggtttct gccggtcact ccaacgcgac gttgaaagaa
    16021 gcgaaagccg gtttccgcgc ggggattacc tttgccaccc atctgtacaa cgcgatgccg
    16081 tatattaccg gtcgtgaacc gggcctggcg ggcgcgatcc tcgacgaagc tgacatttat
    16141 tgcggtatta tcgctgatgg cctgcatgtt gattacgcca acattcgtaa cgctaaacgc
    16201 cttaaaggcg acaaactgtg tctggttacc gacgccaccg cgccagcagg tgccaacatt
    16261 gaacagttca tttttgcggg taaaacaata tactaccgta acggactttg tgtggatgag
    16321 aacggtacgt taagcggttc atccttaacc atgattgaag gcgtgcgtaa tctggtcgaa
    16381 cattgcggta tcgcactgga tgaagtgctg cgtatggcga cgctctatcc ggcgcgtgcg
    16441 attggcgttg agaaacgtct cggcacactc gccgcaggta aagtagccaa cctgaccgca
    16501 ttcacacctg attttaaaat caccaagacc atcgttaacg gtaacgaggt cgtaactcaa
    16561 taagagaaag tatgacacca ggcggacaag ctcagatagg taatgttgat ctcgtaaaac
    16621 agcttaacag cgcggcggtt tatcgcctga ttgaccagta cgggccaatc tcgcggattc
    16681 agattgccga gcaaagccag cttgcccccg ccagcgtaac caaaattacg cgtcagctta
    16741 tcgaacgcgg gctgatcaaa gaagttgatc agcaggcctc caccgggggc cgccgcgcta
    16801 tctccatcgt caccgaaacc cgcaatttcc acgcaatcgg cgtacggctt ggtcgtcacg
    16861 acgccaccat caccctgttt gatctcagca gcaaagtgct ggcagaagaa cattacccgc
    16921 tgccggaacg tacccagcaa acgctggaac atgccctgtt gaatgccatt gctcagttta
    16981 ttgatagcta ccagcgcaaa ctgcgcgagc tgatcgcgat ttcggtgatc ctgccagggc
    17041 ttgttgaccc ggacagcggc aaaattcatt acatgccgca tattcaggta gaaaactggg
    17101 ggctggtaga agctctggaa gaacgtttta aagtgacctg tttcgttggt cacgatatcc
    17161 gtagtctggc gctggcggag cactacttcg gtgcaagtca ggattgcgaa gactccattc
    17221 tggtgcgtgt ccatcgcgga accggggccg ggattatctc taacgggcgc atttttattg
    17281 gccgcaacgg taacgtcggt gaaattggcc atattcaggt cgaaccgctg ggtgaacgct
    17341 gccactgcgg caactttggc tgcctggaaa ctatcgccgc caacgctgcc attgaacaac
    17401 gggtgttgaa tctgttaaag cagggctacc agagccgcgt gccgctggac gactgcacca
    17461 tcaaaactat ctgcaaagcc gcgaacaaag gcgatagtct ggcgtcggaa gtaattgagt
    17521 atatcggtcg tcatctgggt aaaaccatcg ccattgctat caacttattt aatccgcaaa
    17581 aaattgttat tgccggtgaa atcaccgaag ccgataaagt gctgctccct gctattgaaa
    17641 gctgcattaa tacccaggcg ctgaaggcgt ttcgcactaa tctgccggtg gtacgttctg
    17701 agctggatca ccgctcggca atcggcgctt ttgcgctggt aaaacgcgcc atgctcaacg
    17761 gtattttgct ccagcatttg ctggaaaatt aatgtgcttt tatagtggcg cttattgttg
    17821 tcaatattct gggtagtcca tgaccattaa aaatgtaatt tgcgatatcg acggcgtgct
    17881 gatgcacgat aacgtcgccg taccgggtgc agcggaattt ttgcacggga ttatggataa
    17941 aggcctgccg ctggtgttgc tgaccaacta tccttcgcag actgggcaag atctggcgaa
    18001 ccgctttgcc accgcaggtg tcgatgtacc tgacagcgtg ttttatacct ctgcgatggc
    18061 gactgccgat ttcctgcgtc gccaggaagg caagaaagcg tatgtggtgg gcgaaggcgc
    18121 actgattcat gaactgtaca aagccggttt cactattacc gatgtgaacc ctgatttcgt
    18181 gattgttggc gaaacgagtt cctacaactg ggacatgatg cataaagcag cctatttcgt
    18241 cgctaacggt gcacgtttta tcgccaccaa tccggacacc cacgggcgcg gtttttatcc
    18301 cgcgtgtggc gcgttgtgtg caggcatcga aaaaatctcc gggcgtaaac cgttctatgt
    18361 tggtaaaccg agtccgtgga tcatccgcgc ggcattaaac aaaatgcagg cgcattcgga
    18421 agaaacggtg attgtcggcg ataacctgcg taccgatatt ctggcgggct tccaggcagg
    18481 tctggaaacg attctggtgc tttctggtgt ttcatcgctc gacgatatcg acagtatgcc
    18541 gttccgccca agctggattt acccgtcggt cgctgaaatc gacgttatct gaatagcaac
    18601 cacgtctgcg gacgtggttg gctctgatga caaacgccaa actgcctgat gcgctacgct
    18661 tatcaggcct acgcagctcc tgcaatatat tgaattttca tgcttttgta ggccggataa
    18721 ggcgttcacg ccgcatccgg catgaacaaa ttgcactttg tcagcaattt ggcccccgta
    18781 aaatacaaac attcaataaa acccgctgaa aattatcgat tcatcaataa atatatcctc
    18841 acaccagacc ttttttcacc aatcgacaat caccattacg ttttttattt tttcaccgcc
    18901 aaatcgcttg cgcacagtgc tgatttgcgg cattttttta aacaagcaaa cacaacaagc
    18961 aacaaatacc agattaacgg agaaggttat gtgttcaatt tttggcgtat tcgatatcaa
    19021 aacagacgca gttgagctgc gtaagaaagc actcgagctg tcacgcctga tgcgtcatcg
    19081 tggcccggac tggtccggta tttatgccag cgataacgcc attctcgctc acgaacgtct
    19141 gtcaattgtt gacgttaacg cgggggcgca acctctctac aaccaacaaa aaacccacgt
    19201 actggcggta aacggtgaaa tctacaacca ccaggcattg cgcgctgaat atggcgatcg
    19261 ttatcagttc cagacagggt ctgactgtga agtgatcctc gcgctgtatc aggaaaaagg
    19321 gccggaattt ctcgacgact tgcagggcat gtttgccttt gccctgtacg acagcgaaaa
    19381 agatgcctac ctgattggtc gcgaccatct ggggatcatc ccactgtata tgggctatga
    19441 cgaacacggt cagctgtatg tggcctcaga aatgaaatcg ctggtgccag tttgccgcac
    19501 gattaaagag ttcccggcgg ggagctattt gtggagccag gacggcgaaa tccgttctta
    19561 ttatcatcgc gactggttcg actttgatgc agtgaaagat aacgtgaccg acaaaaacga
    19621 gctgcgtcag gctctggaag attccgttaa aagccatctg atgtctgatg tgccttacgg
    19681 tgtgctgctt tctggtggtc tggattcctc aattatttcc gctatcacca agaaatacgc
    19741 agcccgtcgc gtggaagatc aggaacgctc tgaagcctgg tggccgcagt tacactcctt
    19801 tgctgtaggt ctgccgggtt caccggatct taaagcagcc caggaagtgg caaaccatct
    19861 gggcacggtg catcacgaaa ttcacttcac tgtacaggaa ggtctggatg ccatccgcga
    19921 cgtgatttac cacatcgaaa cttatgatgt gaccactatt cgcgcttcaa caccgatgta
    19981 tttaatgtcg cgtaagatca aggcgatggg cattaaaatg gtgctgtccg gtgaaggttc
    20041 tgatgaagtg tttggcggtt atctttactt ccataaagcg cccaacgcta aagaactgca
    20101 tgaagagacg gtgcgtaaac tgctggccct gcatatgtat gactgcgcgc gcgccaacaa
    20161 agcgatgtca gcctggggcg tggaagcacg cgttccgttc ctcgacaaaa aattccttga
    20221 tgtggcgatg cgcattaacc cgcaggataa aatgtgcggt aacggcaaaa tggaaaaaca
    20281 catcctgcgt gaatgttttg agtcatacct gcccgcaagc gtggcctggc ggcagaaaga
    20341 gcagttctcc gatggcgtcg gttacagttg gatcgacacc ctgaaagaag tggcggcgca
    20401 gcaggtttct aatcagcaac tggaaactgc ccgcttccgc ttcccgtaca acacgccaac
    20461 ctcaaaagaa gcgtatctgt accgggagat ctttgaagaa ctgttcccgc ttccgagcgc
    20521 cgctgagtgc gtgcctggcg gtccttccgt cgcgtgttct tccgctaaag cgatcgaatg
    20581 ggatgaagcg ttcaagaaaa tggacgatcc atctggtcgt gcggttggtg ttcaccagtc
    20641 ggcatataag taagaaatgc gaaacagccc cggtgaaatc cggggctgtt tcagttattg
    20701 aggtgcttgt tgattctgat atgcagtaag gaaatgttcc gggtttgcca catcactgtt
    20761 tgacaactca tcactgtagg ccagtatttc atagattaat ttcccaccac tattgttgaa
    20821 ttccgaattt catcctgtac gacaaaagaa tggctgtagg gtggggaaaa aacacaaagt
    20881 aaacaataat tgacgaatat agcgccacgc tgttcgcaac ctaaccaaat agtcactttc
    20941 gagcaatttt ccttgaaaaa gaggttgacg ctgcaaggct ctatacgcat aatgcgcccc
    21001 gcaacgccga taaggtatcg cgaaaaaaaa gatggctacg tagctcagtt ggttagagca
    21061 catcactcat aatgatgggg tcacaggttc gaatcccgtc gtagccacca tctttttggc
    21121 gggagtggcg aaattggtag acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag
    21181 ttcaagtctc gcctcccgca ccattcacca gaaagtgttg tacggatggg gtatcgccaa
    21241 gcggtaaggc accggttttt gataccggca ttccctggtt cgaatccagg taccccagcc
    21301 atcttcttcg agtaagcggt tcaccgcccg gttattgggg tatcgccaag cggtaaggca
    21361 ccggtttttg ataccggcat tccctggttc gaatccaggt accccagcca tcgaagaaac
    21421 aatctggcta cgtagctcag ttggttagag cacatcactc ataatgatgg ggtcacaggt
    21481 tcgaatcccg tcgtagccac caaattctga atgtgtcgaa tacgttcggc aaattcaaaa
    21541 accaatttgt tggggtatcg ccaagcggta aggcaccgga ttctgattcc ggcattccga
    21601 ggttcgaacc ctcgtacccc agccaattta ttcaagacgc ttacatcgta agtgcaccca
    21661 gttggggtat cgccaagcgg taaggcaccg gattctgatt ccggcattcc gaggttcgaa
    21721 tcctcgtacc ccagccacat taaaaaagct cgcttcggcg agctttttgc ttttctgcgt
    21781 tcattcaatg tcgaatgcga tgttgacacg tcttatcctt caatgtcgga tgcgacgctg
    21841 ccgcgtctta tccgacctac ggttggcacg catccggcaa tgttgtaagg ctacaaccct
    21901 aacgcatatt tcagcgcctg acgtttcaac acgccagcac gctctgccgc cattaacccg
    21961 agattacgca caaaacgcag cggcggcaga ttattactga atccggcata aaacagatcc
    22021 ataccacttt gcataatgaa gttatccgcc atgcgccgca tctggtaacg cttgaggaca
    22081 ggataactgg cccacgcttc accgtaactg cgggcattca ccagaacatc aatcagggca
    22141 tcgacatcac gataaccaag attcaccccc tgccccgcca gcggatggat ggtatgcgcg
    22201 gcatcgccca ccagcgcaag ccccggctgc acatactgca acgcatgtcg gcgcgtcagc
    22261 ggaaacgcac cagcggcaag cggtgtaacg taacccagac gcgacgggaa atgcttcgcg
    22321 atttccgcct ggagttgcgc catattcata ttctgcaact ggcgaatacg cgccggagag
    22381 tcataccaca ccagcgatgc ccagttatca aacaacggca gaaacgcacg cggtccgtcc
    22441 ggggtaaatt gctgccaggt gctgtcgccg ggatcgttct cacactgtac gctaatcaac
    22501 atgcacgact gcgcatactg ccaggcatga acgccaattc ccgccatctg ccgcacctga
    22561 gaatttgcgc cgtcggcacc aatcaccagt ttcgcgcgga tcgtttcacc gcccttcagt
    22621 tccagctcct gaagatcatt atctcgatgt aatgcaatca gcgagcctgg cacacgtaac
    22681 gttactttcg gatgcgcttc cagcgcctgc cacagcgcct gttgcaggac ggtgttttcc
    22741 accatatagc caagcaacgg tagcttaagt tcagcggcgt caaacaccac atgcgccgtt
    22801 tcccactccc acgtttccag tctgcggtaa ggatggcaac gcatagcctg tactgcatcc
    22861 cagaccccta accctttaag caatgatacc gatgcggcgc tgatcgccga gatccgcacg
    22921 tccggttggc tatcagcgac aaacggtgca ggttctgcgt gctcgatcac cgttaccgaa
    22981 aatccgtgct gtgccagccc cagcgccagc gcgccgccga ccattcctcc gccgacaatg
    23041 gcaatttccg ttggttgatt tgtcatggtc aatcatcctg ttagcaatat gctttaagtt
    23101 tacaggattt ttacccccaa gggctgataa cgctcatact ggccacaacg gcagcaaagc
    23161 attacactat gcgccctgca ttcctggcta ctatttcgca agagcaagtc gatgaccaaa
    23221 aaactccata ttaaaacctg gggctgtcag atgaacgagt acgattcatc gaagatggcc
    23281 gatctgctgg atgccaccca cggctatcaa ctgaccgacg tggcggaaga agcggatgtg
    23341 ctgctgctga acacctgctc aatccgcgag aaggctcagg aaaaagtctt ccatcagttg
    23401 ggtcgctgga aactgttaaa agagaagaat ccagacctga ttatcggcgt cggtggctgc
    23461 gtggcatcgc aagaaggcga gcacattcgc cagcgcgccc actatgtcga tattattttt
    23521 gggccgcaaa cgctgcaccg tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc
    23581 ccggttgtag atatcagctt cccggaaatc gagaagtttg accgtctacc ggaaccgcgc
    23641 gccgaagggc cgaccgcgtt tgtctccatc atggaaggct gcaataaata ttgcacctac
    23701 tgcgtggtgc cttacacccg tggtgaagag gtaagccgtc cgtctgacga tattctgttt
    23761 gagattgccc agcttgcggc tcagggcgtg cgtgaagtca acctgctcgg tcagaacgtg
    23821 aacgcctggc gtggtgaaaa ctacgacggc tccaccggat cgtttgccga tctgctgcgt
    23881 ctggttgctg cgatcgacgg gatcgatcgt attcgcttta ccaccagcca tccgatcgaa
    23941 ttcaccgacg atatcatcga agtgtatcgc gacacgccgg agctggtgag cttcctgcat
    24001 ctgccggtac agagcggttc cgatcgcatt ctgaacctga tgggccgtac tcatacggca
    24061 ctggagtaca aagcgatcat ccgtaaactg cgtgcggcgc gtccagatat tcagatcagt
    24121 tctgatttca tcgttggctt ccctagcgaa accaccgaag acttcgagaa aacgatgaag
    24181 ctgattgccg acgtcaattt cgacatgagc tacagcttta tcttctctgc acgtccgggt
    24241 acaccagccg ccgatatggt tgatgatgtt ccggaagaag agaagaagca gcgtctgtat
    24301 attctgcaag aacgtatcaa tcagcaagcg atggcatgga gccgccggat gctcggcacc
    24361 acccagcgta ttctggtaga agggacttcg cgtaagagca tcatggagct ttccgggcgt
    24421 accgaaaata accgcgtggt caacttcgag ggcacaccgg atatgatcgg taaattcgtc
    24481 gatgtagaaa ttaccgacgt ctacccgaac tctctgcgcg gtaaagtggt gcgtactgaa
    24541 gatgaaatgg gcctgcgcgt ggcagaaaca ccggaatcgg tgattgcccg tacccgcaaa
    24601 gaaaacgacc ttggcgtggg ttattatcag ccgtaattct caggcccgcc gttccggtgg
    24661 gccttgtttt ttctctcttt atccccatct ttatcgcaat gcttgcgcct ttttcctgtg
    24721 gcgtgaataa tttccttata ggccggtgaa tgttcagctt ccggcggcat acagataaga
    24781 ggaacggttt gaacatagac actcgcgaaa tcaccctgga gccagcagac aacgcgcgtc
    24841 tgttgagcct gtgcggcccg tttgatgaca acatcaagca gctcgaacgc cgtctcggca
    24901 tcgagatcaa tcgccgcgac aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg
    24961 ctgcggcaga cattctgcgt agcctgtatg tcgatacagc cccgatgcgc ggtcagattc
    25021 aggatatcga accggaacag atccacctta cgattaaaga agcgcgagta ctggagcaaa
    25081 gtgcggagag cgtgccggag tacggcaaag cggtcaatat caaaaccaaa cgcggcgtaa
    25141 ttaagccgcg cacgccaaac caggcgcagt acatcgccaa tattctcgac catgacatta
    25201 ccttcggcgt tggcccggcg ggtacgggta aaacctacct ggcagtggct gcggcagttg
    25261 atgccctgga gcgtcaggag attcgccgta ttctgctgac tcgtccggcg gtcgaagccg
    25321 gtgagaaact gggcttcctg cctggcgatt taagccagaa agtagacccg tatctgcgcc
    25381 cactgtacga cgcgctgttt gaaatgctgg gctttgagaa agttgagaaa ctgattgagc
    25441 gcaacgttat tgaagtcgcg ccgctggcct atatgcgtgg tcgtacgctg aacgacgcgt
    25501 ttatcattct cgatgagagc cagaacacca ccatcgaaca gatgaagatg ttcctgaccc
    25561 gtatcggttt taactcaaaa gcggttatca ccggcgacgt cacacagatc gacctgccgc
    25621 gtaatactaa atcaggctta cgtcacgcca tcgaagtact ggccgatgtc gaagagatca
    25681 gctttaactt cttccacagc gaagacgtgg ttcgtcaccc tgtggtggcg cgtatcgtta
    25741 acgcctatga agcctgggaa gaagccgaac aaaaacgaaa agcggcgctg gcggcagaac
    25801 gcaagcgcga agaacaggaa caaaaatgag tcaggtgatc ctcgatttac aactggcatg
    25861 tgaagataat tccgggttac cggaagagag ccagtttcaa acatggctga atgcggtgat
    25921 cccgcagttt caggaagaat cggaagtgac tattcgcgtg gtcgataccg ccgaaagcca
    25981 cagtctgaat ctgacctatc gcggtaagga taagccgacc aacgtgctct ccttcccgtt
    26041 tgaagtgccg cctggcatgg aaatgtcgct actgggcgat ctggttatct gccgtcaggt
    26101 ggttgagaag gaagctcagg agcaaggcaa accactggag gcgcactggg cgcatatggt
    26161 ggtgcacggc agtctgcatt tgttaggtta cgatcacatc gaagatgacg aagcagaaga
    26221 aatggaagcc ctcgaaacag agattatgct tgctctgggc tatgaggatc cgtacattgc
    26281 cgagaaagaa taatttgcca ggcatttgac tggcagacgt ccccgccgtt ggtgattaac
    26341 gacggcgttt tgattaacta acatgagaac ccatacgacg ccatgagcga cgacaattca
    26401 cacagtagtg acactataag caacaagaag ggatttttct ccctgttact cagccaactt
    26461 ttccacggtg aaccgaaaaa ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag
    26521 aacgacctta tcgacgaaga tacgcgcgat atgctcgaag gggtgatgga catcgcagac
    26581 caacgcgttc gcgacatcat gatcccccgc tcccagatga ttaccctgaa acgcaaccag
    26641 acgctggacg aatgccttga tgtcatcatc gagtccgccc actcacgttt cccggtgatt
    26701 agcgaagaca aagatcacat tgaagggatt ctgatggcga aagacttgct gccgtttatg
    26761 cgcagcgatg ctgaagcctt cagcatggac aaagtgttac gtcaggcggt tgtcgttcct
    26821 gaaagtaagc gcgtagaccg gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg
    26881 atcgtcattg acgaattcgg tggggtttcc ggtctggtga ccattgaaga catcctggaa
    26941 ctgattgttg gtgagattga agacgaatat gacgaagaag atgatatcga cttccgtcag
    27001 ctgagtcgtc atacctggac cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg
    27061 ttcggcaccc actttagcga tgaagaagtc gacactatcg gtggtctggt gatgcaggcg
    27121 tttgggcatc ttccggcgcg tggcgaaact atcgacatcg acggttacca gttcaaagtg
    27181 gcgatggccg acagtcgacg tattattcag gttcatgtca aaatcccgga tgactcaccc
    27241 cagccgaagc tggatgaata aaaccgaaac tggatagata actacatggc ttttgcctca
    27301 ttaattgaac gccagcgcat tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg
    27361 ctggccttct ctccttacga cgtctggcct gcggcgatta tttcgttgat ggggcttcag
    27421 gcgttgacct ttaaccgccg tccactccag tctgccgcta ttggcttttg ctggggattt
    27481 ggcctctttg gcagcggtat taactgggtc tatgtcagca tcgcgacctt tggcggaatg
    27541 cctggcccgg ttaacatctt cctggtggtg ctgctggcgg cgtatttgtc gctgtatacc
    27601 ggactgtttg ctggcgtgct gtcgcgtctg tggccgaaaa ccacctggct gcgcgtggcg
    27661 attgccgccc ctgctctctg gcaagtgacc gagtttctgc gcggttgggt actgacaggc
    27721 ttcccgtggt tacagttcgg ctatagccag attgatggcc cgttaaaagg gctggcaccg
    27781 ataatgggcg tggaagccat taacttcctg ctgatgatgg ttagtggcct gctggcactg
    27841 gcgttggtca aacgcaactg gcgtccgctg gtggtggccg tcgtgctgtt tgccctaccc
    27901 ttcccgctgc gttacatcca atggtttacc ccgcaaccgg agaaaaccat tcaggtttcg
    27961 atggttcagg gcgatattcc gcaatcactg aaatgggatg gagatcagct tcttaatacg
    28021 ctgaagattt actacaacgc aacggctccg ctgatgggca aatcatcgtt gattatctgg
    28081 ccggagtcgg cgataaccga tctggaaatt aatcagcaac cgttcctcaa agcactggac
    28141 ggtgagttgc gcgataaagg tagctcgctg gtaaccggga ttgtcgacgc gcgtctcaat
    28201 aagcagaacc gctacgatac ctacaacacc atcatcacac tgggtaaagg tgcaccgtac
    28261 agctacgaat cagccgatcg ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc
    28321 ccgctggagt cgattctgcg tccgttagca ccgttctttg atctgccgat gtcgtcgttc
    28381 agccgtgggc catatatcca gccgccgctg tcagtaaatg gtattgagct tactgcggct
    28441 atttgctacg agatcattct cggcgagcaa gtgcgcgata acttccgccc ggataccgac
    28501 tatctgctga ctatctccaa cgatgcgtgg tttggtaaat ctattggtcc atggcaacac
    28561 ttccagatgg cgcgaatgcg tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac
    28621 aacggcatta cggcggtgat tggcccgcag ggtgagattc aggcgatgat cccgcagttc
    28681 acccacgagg tgttaaccac taatgtgaca ccgaccaccg gactcacacc gtatgcacgt
    28741 accggcaact ggccactgtg ggtgctgacg gcactgtttg gttttgccgc tgtgttgatg
    28801 agtctgcgag cacgtaaacg ttgatgtgcc tgatgtatcc tgcatgagca aaacgtactt
    28861 tctcattgag ctttggggca tggttatcat gccccttttt cttacggttc agggatacaa
    28921 gccacattgc catattgggg atgacgaata attgcctgcg gatcaagcga gcgcgtcctg
    28981 tccatttgga tcaacccgtt cttcgccatg acaaatattt ttctaccatc cgctgaaaa
//
LOCUS       DAJTSL010000035        28928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTSL010000035 DAJTSL010000000
VERSION     DAJTSL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.035"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            33..476
                     /locus_tag="OO433_001390"
     CDS             33..476
                     /locus_tag="OO433_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6049924.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLTDVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            712..1530
                     /gene="ybhA"
                     /locus_tag="OO433_001391"
     CDS             712..1530
                     /gene="ybhA"
                     /locus_tag="OO433_001391"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR6049925.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDMPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1531..2589)
                     /gene="modC"
                     /locus_tag="OO433_001392"
     CDS             complement(1531..2589)
                     /gene="modC"
                     /locus_tag="OO433_001392"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6049926.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2592..3281)
                     /gene="modB"
                     /locus_tag="OO433_001393"
     CDS             complement(2592..3281)
                     /gene="modB"
                     /locus_tag="OO433_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6049927.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3281..4054)
                     /gene="modA"
                     /locus_tag="OO433_001394"
     CDS             complement(3281..4054)
                     /gene="modA"
                     /locus_tag="OO433_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6049928.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4220..4369)
                     /gene="acrZ"
                     /locus_tag="OO433_001395"
     CDS             complement(4220..4369)
                     /gene="acrZ"
                     /locus_tag="OO433_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6049929.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4498..5286
                     /gene="modE"
                     /locus_tag="OO433_001396"
     CDS             4498..5286
                     /gene="modE"
                     /locus_tag="OO433_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6049930.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNANNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5354..6826
                     /gene="modF"
                     /locus_tag="OO433_001397"
     CDS             5354..6826
                     /gene="modF"
                     /locus_tag="OO433_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6049931.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKILLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQHLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7087..8103
                     /gene="galE"
                     /locus_tag="OO433_001398"
     CDS             7087..8103
                     /gene="galE"
                     /locus_tag="OO433_001398"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6049932.1"
                     /translation="MRVLLTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGIGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8113..9159
                     /gene="galT"
                     /locus_tag="OO433_001399"
     CDS             8113..9159
                     /gene="galT"
                     /locus_tag="OO433_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6049933.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNHDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9163..10311
                     /gene="galK"
                     /locus_tag="OO433_001400"
     CDS             9163..10311
                     /gene="galK"
                     /locus_tag="OO433_001400"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6049934.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10305..11345
                     /gene="galM"
                     /locus_tag="OO433_001401"
     CDS             10305..11345
                     /gene="galM"
                     /locus_tag="OO433_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6049935.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11547..12299
                     /gene="gpmA"
                     /locus_tag="OO433_001402"
     CDS             11547..12299
                     /gene="gpmA"
                     /locus_tag="OO433_001402"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6049936.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12457..13509)
                     /gene="aroG"
                     /locus_tag="OO433_001403"
     CDS             complement(12457..13509)
                     /gene="aroG"
                     /locus_tag="OO433_001403"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6049937.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13825..14205
                     /locus_tag="OO433_001404"
     CDS             13825..14205
                     /locus_tag="OO433_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6049938.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAATDAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14319..15260
                     /gene="zitB"
                     /locus_tag="OO433_001405"
     CDS             14319..15260
                     /gene="zitB"
                     /locus_tag="OO433_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6049939.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHAHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15257..15976)
                     /gene="pnuC"
                     /locus_tag="OO433_001406"
     CDS             complement(15257..15976)
                     /gene="pnuC"
                     /locus_tag="OO433_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6049940.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMLLMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSCALSH"
     gene            complement(16014..17057)
                     /gene="nadA"
                     /locus_tag="OO433_001407"
     CDS             complement(16014..17057)
                     /gene="nadA"
                     /locus_tag="OO433_001407"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6049941.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17306..17381)
                     /locus_tag="OO433_001408"
     tRNA            complement(17306..17381)
                     /locus_tag="OO433_001408"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17346..17348),aa:Lys,seq:ttt)
     gene            complement(17528..17603)
                     /locus_tag="OO433_001409"
     tRNA            complement(17528..17603)
                     /locus_tag="OO433_001409"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17568..17570),aa:Lys,seq:ttt)
     gene            complement(17607..17682)
                     /locus_tag="OO433_001410"
     tRNA            complement(17607..17682)
                     /locus_tag="OO433_001410"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17647..17649),aa:Val,seq:tac)
     gene            complement(17734..17809)
                     /locus_tag="OO433_001411"
     tRNA            complement(17734..17809)
                     /locus_tag="OO433_001411"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17774..17776),aa:Lys,seq:ttt)
     gene            complement(17812..17887)
                     /locus_tag="OO433_001412"
     tRNA            complement(17812..17887)
                     /locus_tag="OO433_001412"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17852..17854),aa:Val,seq:tac)
     gene            complement(17923..17998)
                     /locus_tag="OO433_001413"
     tRNA            complement(17923..17998)
                     /locus_tag="OO433_001413"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17963..17965),aa:Lys,seq:ttt)
     gene            complement(18163..18954)
                     /gene="cpoB"
                     /locus_tag="OO433_001414"
     CDS             complement(18163..18954)
                     /gene="cpoB"
                     /locus_tag="OO433_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6049942.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18964..19485)
                     /gene="pal"
                     /locus_tag="OO433_001415"
     CDS             complement(18964..19485)
                     /gene="pal"
                     /locus_tag="OO433_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6049943.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19520..20812)
                     /gene="tolB"
                     /locus_tag="OO433_001416"
     CDS             complement(19520..20812)
                     /gene="tolB"
                     /locus_tag="OO433_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6049944.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPSAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLVFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20945..22126)
                     /gene="tolA"
                     /locus_tag="OO433_001417"
     CDS             complement(20945..22126)
                     /gene="tolA"
                     /locus_tag="OO433_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6049945.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAVKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAK
                     AEAEKKAAAEKAAADKKAAADKKAAEKAAAEKAAADKKAAAEKAAAEADDIFGELSSG
                     KNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKT
                     CTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFK
                     P"
     gene            complement(22191..22619)
                     /gene="tolR"
                     /locus_tag="OO433_001418"
     CDS             complement(22191..22619)
                     /gene="tolR"
                     /locus_tag="OO433_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6049946.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22623..23315)
                     /gene="tolQ"
                     /locus_tag="OO433_001419"
     CDS             complement(22623..23315)
                     /gene="tolQ"
                     /locus_tag="OO433_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6049947.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23312..23716)
                     /gene="ybgC"
                     /locus_tag="OO433_001420"
     CDS             complement(23312..23716)
                     /gene="ybgC"
                     /locus_tag="OO433_001420"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6049948.1"
                     /translation="MNTTLFRWPARVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23866..24159)
                     /gene="ybgE"
                     /locus_tag="OO433_001421"
     CDS             complement(23866..24159)
                     /gene="ybgE"
                     /locus_tag="OO433_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6049949.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIIGLIFFFF"
     gene            complement(24159..24272)
                     /gene="cydX"
                     /locus_tag="OO433_001422"
     CDS             complement(24159..24272)
                     /gene="cydX"
                     /locus_tag="OO433_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6049950.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24287..25426)
                     /gene="cydB"
                     /locus_tag="OO433_001423"
     CDS             complement(24287..25426)
                     /gene="cydB"
                     /locus_tag="OO433_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6049951.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25442..27010)
                     /gene="cydA"
                     /locus_tag="OO433_001424"
     CDS             complement(25442..27010)
                     /gene="cydA"
                     /locus_tag="OO433_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6049952.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27858..>28928)
                     /locus_tag="OO433_001425"
     CDS             complement(27858..>28928)
                     /locus_tag="OO433_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6049953.1"
                     /translation="IEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVI
                     RYDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPT
                     PFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAV
                     FSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLS
                     CRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMP
                     PVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEEN
                     QGSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         7301 a         7611 c         7056 g         6960 t
ORIGIN      
        1 cagacgtcca gaacgaatgg ttcggccttt gcatggatct tgcactggta acaggacagc
       61 gcgaaggaga tctggcggct atgagatggg aggatataag agatggcaga ctatacgtgg
      121 aacaacaaaa aacaggggca aaaattcgca tttctctccc caccacgatt tccagattga
      181 atttaacact taccgatgta ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt
      241 tactcggagg gaagacagcc aggacaatcg cagcacagtt caggattgcc cgagacacat
      301 ccggattgaa atgggaaggt gacccaccgc catttcatga aatccgaagt ttatccggtc
      361 gcctgcactc agcagaaaaa ggcagtgatt tcactcaggc gctccttgga catcgttcat
      421 ccagcatgac ggataaatac cgtgacggac gagggaggga atggaaagac atttagaatt
      481 aatttcgaat gatttcgaaa caccacttgt aattaattga ttataaagga ttaattccta
      541 agaggcattt gcttcatgaa tgctcctttg catttagcaa ctgactgtaa cagctaaaat
      601 tagtcgcttt tggcggtgaa tgctgaatta aaacgcagaa ttttgtccga tagtggtatc
      661 ggtgtaccat tcgcgcagac gaaaattctt aaccttaatc tggaatacgc catgaccaca
      721 cgcgtgattg ctctcgactt agacggcacc ttattgaccc cgaaaaagac cctgcttcct
      781 tcatcgatag aagccctggc ccgcgctcgc gaagcaggct atcaattaat catcgtcaca
      841 ggtcgccatc acgtcgctat tcatcctttt tatcaggcgc tggcgctgga tacacctgct
      901 atttgctgta atggcaccta tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac
      961 ccaatgcccg ttaataaagc cctgcaactc attgagatgc tgaatgaaca ccacattcac
     1021 ggtctgatgt atgtggatga tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc
     1081 acgtctaact gggcgcaaac cctgccgccg gaacagcgtc cgactttcac acaagtcgct
     1141 tctctggctg aaacggcgca acaagttaac gccgtatgga agttcgccct gacgcacgat
     1201 gacatgccgc aattgcagca ttttggtaag catgtcgaac atgaactggg actggagtgt
     1261 gaatggtcct ggcacgatca ggttgatatt gcacgcggcg gcaacagcaa aggtaaacgt
     1321 ttgacgaaat gggttgaggc gcaaggctgg tcgatggaaa acgtcgtggc gttcggcgat
     1381 aactttaatg atatcagtat gctggaggcc gctggtacag gcgtggcgat gggcaacgcc
     1441 gatgacgcgg taaaagcgcg cgccaacatt gtgattggtg ataacaccac cgacagcatt
     1501 gcccagttca tttatagcca cctgatttaa tcaggcggtt atcgacacac ttttaatttg
     1561 cgcgtacagc cacaggcctg gtttgatcgc cagttcatcc ctggcccacg ggctgatacg
     1621 cgcccacagc gttttaccgc cgacttccag ctccacttcc acctgaccgt tgtcgtcata
     1681 actattaaca acttttgccc gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa
     1741 aaccagagaa acatcggaag cctgaatgcg gatacgtagc gcagcttgca gcggttcgtc
     1801 cagcttattg acccacaaat gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg
     1861 atgatgctca agcaccgtca ctttcagaat gctactttgt tgctctttcg gcagccacgg
     1921 attcatcacg ctactacccc agacttcttc cagtgcgcca aaggctttca cctgaccgtt
     1981 ttccagtacc atcactctgt ctgccagatg gaggatctca tccagcgaat ggctgacata
     2041 caacatcggg atgttgattt cccgcgtcag acgttgcaga taaggcaaca gttcgcgttt
     2101 acgcggaata tccagtgacg ccagcggttc atccagcaac agcaattctg gtgccgtcag
     2161 caaagcccgc ccaatcgcta cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg
     2221 gtcaagcaac ggttcaatgc ctaaaagcgc caccagctta tcgaactgat cgaccatgct
     2281 tttcgccatg ccgtagcgca gattgccacg cactttgtaa tgcggaaaca gccgcgcatc
     2341 ctgaaaaaca tagccaacgc gacgcttttc tggcgtcagg cagatacctt tttcggcatc
     2401 atttagtacc cgaccattga ggacaatcct ccctttttgc gggcgcgtca gtccactgat
     2461 ggcgttaatc agcgaagttt ttccggcacc ggagacgcca aagatagcag tgatgccatt
     2521 ggcgggcagc gtttcattaa tagtcaggca atggttgccc aacgtctggg aaaaattcag
     2581 ttccagcatg attagcgccc cgcccgttca cggctgattc tggccagcca ttctgaaatc
     2641 aacagggaga tcatcgccag cgcaatagaa ataatgcaca gtctcgccgc gccactttcc
     2701 ccgccggggg tctggatcag ggtatacatg gcagagggaa tggttcgcgt ttcgccagga
     2761 atgttcgaca caaaggtgat ggttgcacca aactcaccga gagaacgagc aaaagccagt
     2821 accgtaccaa caataattcc cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc
     2881 cagcgcccgg cacctagtgt tcttgcggcc tgttcgagtt tgacatcaac cccttccagc
     2941 gccagacgaa ttgcccgcac catcagcgga aacgacatga cggcggcagc gagaaccgcg
     3001 ccgcgccagc taaaggcgaa ggtaatacca aaccagtcat acagacgttc accgataaat
     3061 ccgcgccgtc ccatcgaaac taataataag taaccgacga ccacgggcgg taacaccagc
     3121 ggtagatgca gtacgctgtc gagcagagct ttgcccggaa acgtgcaacg caccagtaac
     3181 caggcaaaaa agatcccaaa cggcaggcta aacagcacag ccagggaaga aactttcagg
     3241 cttaataaaa ctgcctgcca ttctggatcg gtcagtatca ttacttggtt gtaaatccgt
     3301 aacgtttaaa gatttccgct gcctgcggtc ccttcagata atcataaaat gctttcactg
     3361 tcgcattgtt atgcccttcc acaacagcaa ccggatattc cactttttta tgtgaatctt
     3421 ccgggaaggt ggcaaccact tttacccctt tgctgacaac tgcgtcagaa ccgtaaacaa
     3481 tgcccaacgg cgcttcattg cgttcgacca acgccagcgc gccacgaaca tcttccgccg
     3541 gagccaattt cggggagagc gtatcccatg cgcccagttt ttgcagtgct tcttttgcat
     3601 aaatgccagc agggacatgt tccggatcgc caaccgcgag gcgaccgcca ttcagcagcg
     3661 aagtccagtt agttgtgctg tcgatgatga aatctttctg ctcgctggct ttcggtgcta
     3721 caacgaccag gctattgccg agcagtgtct ggcgcgaagc cgtatcgatc gcttttttat
     3781 caaccgcata atccatccat ttctgatcgg cagagataaa cagatccgca ggcgctcccg
     3841 cttcaatctg acgggccaga gtagatgacg aagcgaaaga agaaaccaca tccacgcctt
     3901 tttctttttt atactgcgta gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga
     3961 acaccgtgat tttcccttca tctgccagtg tattgccagc aacagcgaaa gagagtgccg
     4021 ccccggcaaa caagttcaac catttacgag ccattggtaa ccccttaatg taacgttata
     4081 taggtgacaa tataacggct aactcagaat tttcccagca gttattgcta acctttgtga
     4141 ggtagataag aaaaaatatc ggctggaaaa gcaggaagtt gagaaaaaga aatgcccgac
     4201 taagtgggca ttcagggaat caatgatttt gtccgggctg gtctttttta ccaacaccag
     4261 aaaagatgtt gaatacttca ccaagaccgt aaatcagacc caggatgatg gccatcacga
     4321 caggtaccat gattacggcg aataccagac tttttaataa ctctaacatg gtcaactcca
     4381 gatatagtca tgagactatt ctaaccgcta agcacagaaa agcactcccc ttttgtgcgg
     4441 tcagctttgc gtggcgttcg ttttccgtca caataagact tttgccagga cattgttatg
     4501 caggccgaaa tccttctcac ccttaagctc caacaaaaat tattcgccga cccgcgccgg
     4561 atttcgctac taaaacacat tgcgctttcc ggttccatta gccagggagc gaaagatgcc
     4621 ggtatcagct ataaaagcgc ctgggatgcc attaacgaga tgaatcagtt aagcgagcat
     4681 attctggttg agcgcgcaac aggcggtaaa ggtggcggcg gcgcagtact gacccgctat
     4741 ggtcagcgac tgattcagct ctatgactta ctggcgcaaa tccagcaaaa agcctttgat
     4801 gtgttaagtg acgatgacgc cctgccgctg aacagcctgc tggccgcgat ctcacgtttt
     4861 tcactgcaaa ccagcgcccg taaccagtgg ttcggtacca tcaccgcccg cgatcatgat
     4921 gacgttcaac agcatgttga tgtcttactg gctgacggaa aaacacgcct gaaagtcgca
     4981 attaccgcac aaagcggcgc acgtctgggg ctggatgaag gcaaagaagt gttgatattg
     5041 ctaaaagcgc cgtgggtagg cattactcag gacgaagcgg tcgcacaaaa cgccaacaac
     5101 caattaccgg gtattattag tcatattgag cgcggcgcag agcagtgcga agtattaatg
     5161 gcgctacccg acgggcaaac actgtgcgcc acagtgccgg taaatgaagc gacttctctt
     5221 cagcaagggc agaatgtcac ggcctacttt aatgccgaca gcgtgattat cgccacgctg
     5281 tgctaagcgt gttgacaatt tgttatgaaa cacgtatccc tgtcagtaat cgctgcacaa
     5341 aatgggatat aaaatgtcat cgttgcaaat tttgcaaggc acgtttcgtc ttagcgacac
     5401 aaaaacgctg caattgcctc agctaacgtt aaacgcgggt gatagttggg cgtttgtcgg
     5461 ttcgaatgga agcgggaaat cggccctggc ccgcgcgctg gcgggggaac ttccgctttt
     5521 gaaaggtgaa cggcaaagcc agttttccca catcactcgt ctctccttcg agcaattgca
     5581 aaaactcgtc agcgacgaat ggcagcggaa taacaccgat atgctcggcc ctggcgaaga
     5641 tgacaccgga cgcactacgg ctgagatcat tcaggatgaa gtaaaggatg caccgcgttg
     5701 catgcaactg gcgcagcagt tcggtattac cgccctcctc gaccgacgct ttaaatacct
     5761 ttccactggc gagacgcgaa aaatcctgct gtgtcaggcg ctgatgtcgg agcctgactt
     5821 gttgattctt gatgagccgt tcgatggcct ggatgttgcc tcacgtcagc agctggctga
     5881 gcgactcgcc tcgttacatc agtccggtat tactctggta ctggtgctca atcgcttcga
     5941 tgagatcccg gagtttgtcc agtttgctgg cgtgctggcg gattgcacgt tagcggaaac
     6001 tggcgctaaa gaggaactgc tccaacaagc actcgtcgcg caactggcac atagtgaaca
     6061 gcttgaaggt gtgcaactgc cggagccgga tgaaccttca gcacgtcacg ccttacccgc
     6121 caacgaaccg cgcattgtgc tgaacaatgg cgtggtttct tataacgatc gccccattct
     6181 taataacctt agctggcagg tgaatccagg tgaacactgg caaattgtcg ggccaaatgg
     6241 tgcaggaaaa tcgacgttat taagcctggt tactggcgat catccgcaag gttacagcaa
     6301 cgatttgacg cttttcggac gacgtcgcgg cagcggcgaa accatctggg atatcaaaaa
     6361 gcatatcggt tacgtcagca gtagtttgca tctggattac cgggtcagca ctaccgtgcg
     6421 taatgtgatt ctttctggct attttgattc gattggcatt tatcaggccg tttcggatcg
     6481 ccagcaaaaa ctggtgcagc agtggctgga tattctcggc attgataaac gtacggctga
     6541 cgctccgttc catagtcttt cctggggaca gcagcatctg gcgctgatcg tccgcgcact
     6601 ggtgaaacat ccgacgttgc ttattctcga tgaaccacta caggggcttg atccgctcaa
     6661 tcgccagctt atccgccgtt ttgttgatgt gctgattagc gaaggtgaaa cgcaattgtt
     6721 gtttgtttcg caccacgctg aagatgcgcc tgcctgtatt acccatcgcc ttgagttcgt
     6781 gccggacggt ggactctatc gctatgtgct gacaaaaata tattgagtcg gtagtgctga
     6841 ccttgccgga ggcggcctaa gcgccctctc cggccaacgg ttcgacgcat gcaggcatga
     6901 aaccgcttct tttttcagat aaaaagcgca atcattcata aaccctctgt tttataatca
     6961 cttaatcgcg cataaaaaac ggctaaattc ttgtgtaaac gattccacta atttattcca
     7021 tgtcacactt ttcgcatctt tgttatgcta tggttatttc ataccataag cctaatggag
     7081 cgaattatga gagttctgct taccggtggt agcggttaca ttggaagtca tacctgcgtg
     7141 caattactgc aaaacggtca tgatgtcatc attcttgata acctctgtaa cagtaagcgc
     7201 agcgtactgc ctgttatcga gcgtttaggc ggcaaacatc caacgtttgt tgaaggcgat
     7261 attcgtaacg aagcgttgat gaccgagatc ctgcacgatc acgctatcga caccgtgatc
     7321 cacttcgccg ggctgaaagc cgttggcgaa tcggtacaaa aaccgctgga atattacgac
     7381 aacaatgtca acggtactct gcgcctgatt agcgccatgc gcgccgctaa cgtcaaaaac
     7441 tttattttta gctcctccgc caccgtttat ggcgatcagc ccaaaattcc atacgttgaa
     7501 agcttcccga ccggcacacc gcaaagccct tacggcaaaa gcaagctgat ggtggaacag
     7561 atcctcaccg atctgcaaaa agcccagccg gactggagca ttgccctgct gcgctacttc
     7621 aacccggttg gcgcgcatcc gtcgggcgat atgggcgaag atccgcaagg cattccgaat
     7681 aacctgatgc catacatcgc ccaggttgct gtaggccgtc gcgactcgct ggcgattttt
     7741 ggtaacgatt atccgaccga agatggtact ggcgtacgcg attacatcca cgtaatggat
     7801 ctggcggacg gtcacgtcgt ggcgatggaa aaactggcga acaagccagg cgtacacatc
     7861 tacaacctcg gcgctggcat aggcagcagc gtgctggacg tggttaatgc cttcagcaaa
     7921 gcctgcggca aaccggttaa ctatcatttt gcaccgcgtc gcgagggcga ccttccggcc
     7981 tactgggcgg acgccagcaa agccgaccgt gaactgaact ggcgcgtaac gcgcacactc
     8041 gatgaaatgg cgcaggacac ctggcactgg cagtcacgcc atccacaggg atatcccgat
     8101 taaggaacga ccatgacgca atttaatccc gttgatcatc cacatcgccg ctacaacccg
     8161 ctcaccgggc aatggattct ggtttcaccg caccgcgcta agcgcccctg gcagggggcg
     8221 caggaaacgc cagccaaaca ggtgttacct gcgcacgatc cagattgctt cctctgcgca
     8281 ggtaatgtgc gggtgacagg cgataaaaac cacgattaca ccgggactta cgttttcact
     8341 aatgactttg cggccttgat gtctgacacg ccagatgcgc cagaaagcaa cgatcctttg
     8401 atgcgttgcc agagcgcgcg cggtaccagc cgggtgatct gcttttcacc ggatcacagt
     8461 aaaacgttgc cagaactgag cgttgcggca ttgacggaaa tcgtcaaaac ctggcaggag
     8521 caaaccgcag agctgggaaa aacgtacccg tgggtgcagg tctttgaaaa caaaggtgcg
     8581 gcgatgggct gctctaaccc gcatccgcac ggacagattt gggcaaatag cttcctgcct
     8641 aacgaagctg agcgcgaaga ccgcctgcaa aaagaatatt ttgccgaaca gaaatcacca
     8701 atgctggtgg attatgttca gcgcgagctg gcagacggta gccgtaccgt tgtcgaaacc
     8761 gaacactggt tagccgttgt gccttactgg gctgcctggc cgttcgaaac gctactgctg
     8821 cccaaagccc acgttttgcg gatcaccgat ttgaccgacg cccagcgcag cgatttggcg
     8881 ctggcgttga aaaagctgac cagtcgttat gacaacctct tccagtgctc cttcccctac
     8941 tctatgggct ggcacggcgc accatttaat ggcgaagaga atcaacactg gcagctgcac
     9001 gcgcactttt atccgcctct gctgcgctcc gccaccgtac gtaaatttat ggttggttat
     9061 gaaatgctgg cagagaccca gcgagacctg accgcagaac aggcagcaga gcgtttgcgc
     9121 gcagtcagcg atatccattt tcgcgaatcc ggagtgtaag aaatgagtct gaaagaaaaa
     9181 acacaatctc tgtttgccaa cgcatttggc taccctgcca ctcacaccat tcaggcgcct
     9241 ggccgcgtga atttgattgg tgaacacacg gactacaacg acggtttcgt tctgccctgc
     9301 gcgattgatt atcaaaccgt gatcagctgt gcaccacgcg atgaccgtaa agttcgcgtg
     9361 atggcagccg attatgaaaa tcagctcgac gagttttccc tcaatgcgcc cattgtcgcg
     9421 catgaaaact atcaatgggc gaactacgtt cgtggcgtgg tgaaacatct gcaactgcgt
     9481 aacaacagct tcggcggtgt ggacatggtg atcagcggca atgtgccgca gggtgccggg
     9541 ttaagttctt ccgcttcact ggaagtcgcg gtcggaaccg tattgcagca gctttatcat
     9601 ctgccgctgg acggcgcaca aatcgcgctt aacggtcagg aagcagaaaa ccagtttgtt
     9661 ggctgtaact gcgggatcat ggatcagcta atttccgcac tcggcaagaa agatcatgcc
     9721 ttgctgattg actgtcgttc actggggacc aaagcagttt ccatgccgaa aggtgtggct
     9781 gtcgtcatca tcaacagtaa cttcaaacgt accctggttg gcagcgaata caacacccgt
     9841 cgtgaacagt gcgaaaccgg tgcgcgtttc ttccagcagc cagccctgcg cgatgtcacc
     9901 attgaagagt tcaacgctgt tgcacatgag ctggacccaa tcgtggcgaa acgcgtgcgg
     9961 catatcctga ctgaaaacgc ccgcaccgtt gaagctgcca gcgcgctgga gcagggcgac
    10021 ctgaaacgta tgggcgagtt gatggcggag tctcatgcct ctatgcgcga tgatttcgaa
    10081 atcaccgtgc cgcaaattga cactctggta gaaatcgtca aagctgtgat tggcgacaaa
    10141 ggtggcgtac gcatgaccgg cggcggattt ggcggctgta tcgtcgcgtt gatcccggaa
    10201 gagctggtgc ctgccgtaca gcaagctgtc gctgaacaat atgaagcaaa aacaggtatt
    10261 aaagagactt tttacgtttg taaaccatca caaggagcag gacagtgctg aacgaaactc
    10321 ccgcactggc acccgatggt cagccgtacc gactgttaac tttgcgtaac aacgcaggga
    10381 tggtagtcac gctgatggac tggggtgcga ctttactttc agcccgtatt ccgctttccg
    10441 atggcagcgt ccgcgaggcg ctgctcggct gtgccagccc ggaatgctat caggatcagg
    10501 ccgcgtttct gggggcctct attggtcgtt atgccaaccg catcgccaat agccgttata
    10561 cctttgacgg tgaaaccgtg accctttcgc caagtcaggg cgttaaccag ctgcacggcg
    10621 gcccggaagg gttcgacaaa cgtcgctggc agattgtgaa ccagaacgat cgtcaggtgc
    10681 tgtttgccct gagttcagat gatggcgatc agggcttccc gggtaatctc ggcgcaacgg
    10741 tgcaatatcg tctgaccgac gataaccgta tctccattac ttatcgcgcc acagttgata
    10801 aaccttgccc ggtgaatatg actaatcacg tctatttcaa tcttgacggc gagcagtctg
    10861 acgtgcgcaa tcacaagttg cagattctgg cggacgaata tctgccggtt gatgaaggcg
    10921 gcattccgca cgacggcctg aaatctgtcg ccggaacgtc ttttgatttc cgcagcgcca
    10981 aaatcatcgc cagtgagttt cttgccgacg acgatcagcg caaagtgaaa ggttacgatc
    11041 acgcattctt gttacaggcc aaaggcgatg gcaagaaagt ggcggcgcat gtctggtcag
    11101 cagatgaaaa attgcagctg aaggtctaca ccaccgctcc ggctctgcaa ttctactccg
    11161 gcaacttcct cggcggcaca ccgtcgcggg gaacagaacc ttacgccgac tggcaagggc
    11221 tggcgttgga aagcgagttt ctgccggaca gcccgaacca ccctgaatgg ccgcaaccgg
    11281 actgcttcct gcgtcctggc gaagagtatt ccagcctgac ggaatatcag tttattgctg
    11341 agtaatgtta agccctcgtc atgagggctt tatctcatat tgctcaaatc accagcaaac
    11401 accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact attttcgcta
    11461 tggttatgcg taagcattgc tgttgcttcg tcgcggcaat ataatgagaa ttattatcat
    11521 taaaagatga tttgaggagt aagtatatgg ctgtaactaa gctggttctg gttcgtcatg
    11581 gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg
    11641 agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa ggttacagct
    11701 ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg
    11761 acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac gaacgtcact
    11821 acggtgcgtt gcagggtctg aacaaagcgg aaactgctga aaagtatggc gacgagcagg
    11881 tgaaacagtg gcgtcgtggt tttgcagtga ctccgccgga actgactaaa gatgatgagc
    11941 gttatccggg tcacgatccg cgttacgcga aactgagcga gaaagaactg ccgctgacgg
    12001 aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaact attctaccgc
    12061 gtatgaagag cggtgagcgc gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg
    12121 tgaaatatct tgataacatg agcgaagaag agattcttga gctgaatatc ccgactggcg
    12181 tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat tatctgggta
    12241 atgctgacga gatcgcagcg aaagcagcgg cggttgcaaa ccagggtaaa gcgaagtaaa
    12301 cgtcattcgt ttaaaatgag aaagccgact gcaagtgagt cggctttttt gttgctaaca
    12361 atggagcaca ttgcctgatg cgacgcttcg cgacttatca ggcctgtggt gattcatcgg
    12421 atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg cttttactgc
    12481 attcgccagt tgacgtaaca gagcatcggt atcttcccag ccgatgcagg catcggtgat
    12541 gctcttaccg taggccagcg gctccccgct ctcgaggctc tgattgcctt ccaccagatg
    12601 gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct ggcaaacgtc
    12661 agcacaaaca tccatctgct ttttgaattg tttggacgag ttagcatggc tgaaatcgat
    12721 catcacctgt gctggcaggc ctgctttgtt cagcccttct ttcacttcag caacgtgctt
    12781 cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcgc cgttaccgct
    12841 ggtattcaca atcgccgaat gcccccattt cgttacggac aggaagcagt gcggcgcacc
    12901 ggcggcatta atggcatcga tagccacttt aatcgtaccg tcggtgccat ttttgaagcc
    12961 gactggacaa gaaagccctg atgccagttc gcggtgcacc tgcgattcgg tggtacgtgc
    13021 gccaattgcg ccccagctca tcaggtcagc gagatattgt ggggtgatca tatcgagaaa
    13081 ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac gggctatacg
    13141 aagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca gccctttcca
    13201 gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca gctcatcttt
    13261 cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga caggatcatg
    13321 aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca ggatcttatg
    13381 gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagtagcgg ggaatttttc
    13441 cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat cgtcgttctg
    13501 ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat acttccaatc
    13561 tatatcttcc gtcagaatgt gtaaacgggg ttttacatta tgaacggatt aatcctggaa
    13621 ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa tttctaactg
    13681 taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa actggctgaa
    13741 atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg ttatccgaca
    13801 taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt atttctgact
    13861 gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc gcaaactaat
    13921 aacggccagg caaacgccgc aactgatgcg ggccaggtag cccctgacgc ccgtgaaaat
    13981 gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg cacaatgctg
    14041 cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaga gcacaagaac
    14101 accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaac cggtgatggc
    14161 atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg caaaatagcc
    14221 aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc agtatgatgt
    14281 cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca cactcacaca
    14341 cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct ttcggcgtga
    14401 ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct ctggcattgc
    14461 tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc ctactcgccg
    14521 tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta agactgacca
    14581 cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg attgtctggg
    14641 aggcgataga acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg atggcaattg
    14701 ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac ggcagcgaag
    14761 agaaaaacct caacgtacga gcagcggcat tgcatgtgct cggggacctg ttgggttcgg
    14821 ttggggctat tattgccgcg ttgattatta tctggaccgg ctggacacct gctgacccca
    14881 ttctctcgat agtggtgtcg cttctggttc tgcgtagcgc gtggcgactg ttgaaagata
    14941 gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa ctgaagcgtc
    15001 gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta tggatggtag
    15061 gtgagaagcc ggtgatgacg ctgcatgtgc aggtgatccc gccacacgct cacgatgcct
    15121 tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac gccaccattc
    15181 agatggaata tcaaccttgt catgggccag actgccacct gaatgaaggg gtgtctggcc
    15241 attcacatca tcaccattaa tgggacagcg cgcatgagcc tctttcacgt gcgctgttga
    15301 tccacatccg actgccgttg agcgcaataa aggtcaggat gatgtactcc agagacatgg
    15361 cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc acccacaaca
    15421 gccagttttc cacatactta cgcgtcatca gaagcattgc cacgatagat aacaccatca
    15481 tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca acctgtaatc
    15541 ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc ggattgataa
    15601 ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagc gccttcggca
    15661 atggtaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc gaccacgcat
    15721 accaaccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg gcatacagct
    15781 gaatctgaaa gaaaataatg ccaaacaagg tgacgttgat caggccaaag aagtagttgc
    15841 tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg cctaccgctt
    15901 cgatccatga gagatcataa ccgcctgctc ctattggtat atgtaccagg atattctgca
    15961 cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat tcgttatcca
    16021 cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct ctctcgcagc
    16081 ctttcatcaa catgaacctc gtgattgctt ccttcctgtt ctaatgcctc tgcgatggcc
    16141 tgaaggccat tcatggccat ccacggacaa tgcgcgcagc tgcggcaggt tgcaccctca
    16201 cctgcggttg gtgcttccag taactcttta tctggcaccg cctgctgcat tttgtagaaa
    16261 ataccccgat cggttgccac aataagcctc tgatgtggca atgttttcgc agcagcgatc
    16321 agttgactgg tggaaccgac cgcatccgcc atatcgacaa tagcttgtgg tgattctgga
    16381 tgcaccagta tggcagcatc cgggtattct tcttgcaagc gggttaacgc ctgagtctta
    16441 aattcatcat gcacaataca ggcaccctgc cagcatagaa tgtctccacc cgtctgtttt
    16501 tgcacgtaac gccccagatg tttgtcgggt gcccagatga ttttttcacc caaactatca
    16561 agatgatcaa taagttcgac ggcaatgctt gaagttacca cccaatctgc gcgcgctttt
    16621 accgcagcag aagtgttggc gtagacgacg acagtacgat cgggatgggc atcgcaaaat
    16681 gcgttaaatt cttcaacagg gcagccgaga tccagtgaac attcagcctg aagtgtcggc
    16741 atcagaattg ttttttccgg actgagaatt ttggcggttt ctcccataaa tctcacccca
    16801 gcgactaaca aagtagaagc gggatgcttt gcaccgaagc gcgccatttc cagagaatca
    16861 gaaatacagc caccggtttc ttctgccagt tgttgaattt cgggatcggt atagtagtgg
    16921 gcaaccatca ccgcattacg ttcttttagc agacgtttta tcttctcgcg gtaatacgct
    16981 ttttcatcaa tgcttaacgg cgtcggcttc ggggggaaag gataaatcgc cgtgtctgga
    17041 tcaaacatta cgctcatctt accatctcgt tttacaggct taacgttaaa accgacatta
    17101 gcgtaatatt cgctgttatc ggtgtaattt gttttatatg ctaaacaaga tagtcgaatg
    17161 gtggggaaaa gtcactcgaa ttacgcctaa ttccaccgac gtagagttgc ttgcacagta
    17221 agctctgacg atgtcatcag gtttacaaag tgaaattgag gatgcctgaa tagcaaaaaa
    17281 gcgcctttag ggcgcttttt tacattggtg ggtcgtgcag gattcgaacc tgcgaccaat
    17341 tgattaaaag tcaactgctc taccaactga gctaacgacc catacgggtg ttacctgaaa
    17401 gatttctact cggggccaat ctttaagatt ggtgtgtcgt gcaggatgac tcgcttcgct
    17461 tgccctacgg gccgttgctg gcgcaacgtt atccttcacg cttaacatct gagtttgatg
    17521 ttaaaattgg tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc
    17581 tctaccaact gagctaacga cccgagtggt gggtgatgac gggatcgaac cgccgacccc
    17641 ctccttgtaa gggaggtgct ctcccagctg agctaatcac ccgatactgc gctggatact
    17701 acaaactact tccaccgaac ccatcgtaat tagtggtggg tcgtgcagga ttcgaacctg
    17761 cgaccaattg attaaaagtc aactgctcta ccgactgagc taacgacccg gtggtgggtg
    17821 atgacgggat cgaaccgccg accccctcct tgtaagggag gtgctctccc agctgagcca
    17881 atcacccaat actacgctgg atactacaac taactatgtt agtggtgggt cgtgcaggat
    17941 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccac
    18001 tttttcgttg ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt
    18061 tcagacttga gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg
    18121 acacgaccag aaataatgca acttctggtc gtgtgttatg cattacatcg cgttcagacg
    18181 tttttgtgcc tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac
    18241 ggctttcgct tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc
    18301 atcagcagcc tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc
    18361 atcatctttt ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag
    18421 gtaagttgaa tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg
    18481 gcgggattta tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg catcgccgct
    18541 tttcaccggc gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc
    18601 cgcaccgctt tgatcgctgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc
    18661 gatctgcaac aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc
    18721 ctgaatctga ccacgcaggg aatcaatatc ggattgatta tcagaaagtt gttgctggag
    18781 ttgggttaaa agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc
    18841 gaccgagcct gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc
    18901 tataccaacc agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt
    18961 ctcttagtaa accagtaccg cacgacggtt tttggagtat gccgcttcgt catgacccag
    19021 tactgcaggt ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt
    19081 accctgcagg tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc
    19141 cggagtacca cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg
    19201 caggaagttt gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc
    19261 cagatcgaag taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc
    19321 ttcggaagac atgttgccgt tgccgccgtt cgcatccata ccagtgccgg cacccagcat
    19381 gccttcgctg ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac
    19441 aggcagagca atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt
    19501 actattcaat taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat
    19561 cagttgccgg aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc
    19621 ccatcccctg agaagagctg tagattacca tagtgccatt aggtgccaga cttggcgttt
    19681 catccaggaa cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa
    19741 tgtgctgctg cccaccattg gagctgacca ttaccataaa tttaccgtcg ctgctgacat
    19801 ccgcatcctg gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat
    19861 tcactttata aacctgcgga cgaccggcct ggtcagaagt aaataccagg ttctggctat
    19921 ccgggaacca ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac
    19981 cagaagccaa atccattacg tacaggttca gactaccggt tttcgacaag gcgaatgcca
    20041 gtttgctgcc gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct
    20101 gacgtacagc gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg
    20161 tcacataagc cagtttagaa ccgtctggtg accacgccgg agacatcagc ggctgcggtg
    20221 aacggtgaac gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga
    20281 actgaccgcc gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatgc
    20341 cggtcagttt ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct
    20401 tgttcacttt gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa
    20461 gttgataagc aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag
    20521 cgtcaatgcc cagtgcggac catgcagatg gttgtacttc ctgcgcacta cccggctgct
    20581 gtggcagacg agcgcgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa
    20641 cgatgccgcc aatatcttca ggtgccgcac caggccccgc ccactggaaa ggaacaacac
    20701 caataggacg accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa
    20761 ctgatgccca cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc
    20821 ccttatctgg accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca
    20881 aaactaccag aaccccgtgg caaccggtgc ctgatgttga ccgtccgaac agtcaacatc
    20941 gcgattacgg tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg
    21001 gtggtttcgg gatcttcgca agtttagctg ctgccaacgc agcctgacaa agtgcgggat
    21061 cgccaccttc aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg
    21121 tacaggtttt gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa
    21181 tctgcccggc atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac
    21241 tcccggcagg cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac
    21301 cagagcttag ctcaccgaaa atatcatctg cctctgcggc ggctttttct gccgctgctt
    21361 tcttatcagc tgctgccttt tcagcagccg ctttttctgc tgcttttttg tcggctgcag
    21421 ctttcttatc agctgcagcc ttttcagcag ccgctttctt ctcagcttct gctttggctt
    21481 tttcagcagc ttcagttgcc gctttctttc ttgcttcagc tgcagctgct tctgccgctt
    21541 ccgctttctt cttcagtgcc gcagcggctg cctcggcttt tttctgcgct gcggctttgg
    21601 cggcttctgc ttctgctttt ttctttgcgt ctgcagccgc tttcttcgct gcttcttccg
    21661 cagctttagc atctgcttcg gccttcgctt tagcatctgc cgccgctttc gctgccgcct
    21721 cttcagcttg cttctgcttt aactcggcct gttttacggc ttcttcagcc tgctttttct
    21781 gctcctgagc cgctaaccgc tctttctcaa gttgcttcag gcgttcctgt tcagccgctt
    21841 gtttctcacg cagttcttca gcagcctgct gttccttcat cttgcgctgt tcatcagaac
    21901 gcttcgcgct tgattcctgg ctttgcatgc gtttgtactg ctcaactacc gcacctgaat
    21961 caaccatgac agcgtcgatg gacgaaccac cgccgcctcc ggctgaagct tctatattct
    22021 catcgaacga actccagatc agcgccgcaa ataagatgac atgcagcact gctgaaataa
    22081 ttatcgcccg cttgagcttg tcgttttgtt cggttgcctt tgacactctc ggtttccaaa
    22141 aactgttcgc ctgttacccg ctctctttca agcaagggaa acgcagatgt ttagataggc
    22201 tgcgtcatta aaccaaccga tttcacaccc gcactatgta acaagttcag tgctttaatt
    22261 atttcatcgt aaggcacatc ttttgcgcca ccgatcagaa agaccgtttt cgggttggcc
    22321 ttgaagcggc tggacacttc cgccaccacc tgctctggtg gtaaacgctc caggcgatct
    22381 ttctcaacca ccacggtgta ctgaccaata ccagacactt caacaatcac tggcggatta
    22441 tcgttactgc tcaccgcctg tgattcagta gcgtctggca gatcgacctc cacgctctgg
    22501 gtgatgatgg gcgctgtcgc catgaagatc agcaacagca ccagcagtac gtccagcaac
    22561 ggtacaatgt tgatttcgga cttaagttcg cgacgacctc gtccacgcgc tctggccatg
    22621 acttacccct tattgctctc gctaacggta aacgcctggc ggtgcagaat cgcggtaaac
    22681 tcttccataa agttgtcgta attcagttcc agtttgttta cgcgctggtt gaggcggttg
    22741 taggccataa ctgccgggat agcggcaaac agaccaattg cagtcgcaat caacgcttct
    22801 gcgataccgg gcgcaaccat ttgcagtgtt gcttgtttta ccgccccgag ggcgataaag
    22861 gcatgcatga tcccccagac cgtaccaaac agaccaatat acgggctgat ggagccaacc
    22921 gtaccgagga acggaatgtg cgtttccaga ttttcaagtt cacggttcat ggagatacgc
    22981 atagcacgcg acgccccttc cactacggct tccggcgcat ggctattggc acgatgcagg
    23041 cgcacaaact ctttgaaccc gctgtaaaat atttgttccg aaccagtcag attatcccgt
    23101 ttcccctgac tctcttgata gaggcgagag agttcgattc cagaccagaa tttatcttca
    23161 aacgcttcgg cttcgcgcgc cgctgcgtta agaatacggg tccgctggat aataatggcc
    23221 caggatgcga ttgaaaaacc aatcaaaatc aacatgataa gtttaaccag aaggctagcc
    23281 ttcaggaaca aatcaaggat attcatgtca gtcactgctt aaactccgcg acaatagact
    23341 tgggaagcgc acgaggcttc attttgagtg ggtcaacgca aacaaccaga acctctgctt
    23401 cattcagcaa agtattctcg gcgttgacaa tacgttgcgt gaaaaccaaa gaggtgccac
    23461 gcattgatgt tatttcagtc tgtatttcga gcatatcgtc gagccgcgca ggtgcgtaat
    23521 attccaccgt cattttacgt accacaaagg caacgcgttc agccatcagc gcctgctgac
    23581 tgaagtgatg atgacgcagc atctctgtgc gtgctctttc ataaaaagcg acgtaactgg
    23641 cgtggtacac cacaccaccg gcatcggtat cttcatagta gacgcgagcc ggccatcgaa
    23701 acagcgttgt attcacttta catcccggta atgcaacaaa agttagagct tttaaacgct
    23761 gctactatac gcgcgcgatt gaggtttggg aatgagggca agttaaggga gcgtaaaaat
    23821 ttatgggcct ggagcggccc ataatttttt aaagagaatt ctgatttaaa agaagaagaa
    23881 aatcagcccg ataatgagaa caatatcggc aagcaatggg caaaaaatcc cttgccaaag
    23941 aaccttctgc ggacgaaagc ccacgccgtg aatcacacca gcacagacgg cccacatcag
    24001 caataaacca tgccagattt ccagttcact ggtcttcgcg gcaaaacgag atgggtccca
    24061 aaacatacat cctgctaaca gaagcgccat cacgaaggaa agcgcccgca gggggcgctt
    24121 gtccattacc gcatacaagg tcgcgataat cttactcatc agatgtcttc ttgaccggct
    24181 ttgcctgatt cgacgtgttc aagcgccagc gcggtgatta ccccaaacga acaggcaaga
    24241 agcgttccca gaatccatgc gaaataccac atttttagct ccttacttag tacagagagt
    24301 gggtgttacg ttcaatatct tctttggtga tacgaccgaa cattttccag taacaccagg
    24361 cggtgtagag cagaatgatc ggtaccagaa ccaccgcaac ccaggtcatg acgttaagcg
    24421 tcagctggct ggaagttgca tcccacattg tcagacttgc gttcatcatg gtgctggacg
    24481 gcatcacaaa cgggaacatt gcgataccgg ctgtcaggat gatgcaggcc agcgtcaggg
    24541 aggagaacac aaacgcccac gcggctttat ccatacgtgc agtcaggatg gtcagcagcg
    24601 gcagaaccac acccagtgcc ggaatagccc acagaattgg cgtgttgttg aagttaacca
    24661 gccatgcgcc agcttcacga accacttctt tattcagtgg gttagaggct gcgtaatggt
    24721 ccattgtcga tttcacgaca taaccatcga taccgtacat cacccatacg ccagccagtg
    24781 cgaaacagac cagtgtcacc agcgcagcca cctgagccgt tgcacgggta cgcaggtgca
    24841 gttcgcccac ggtacgcatt tgcagatagg ttgcgccctg agtaatgatc atccctacgc
    24901 tcaccacgcc tgccagcagg ccgaacgggt taagcaactg gaagaagtta ccggtgtagt
    24961 acagacgcag atattcatca acgttgaacg gtacgccctg caacaggtta ccgaacgcaa
    25021 caccaattac cagcggcgga acgaagctac caatgaagat gccccagtcc cacatgttac
    25081 gccagcgggt ttcttcaatc ttggagcggt agtcaaaacc gaccggacgg aagaacaaag
    25141 acgccagcac gaggatcatc gccacataga agccggagaa cgcagcggca tagaccatcg
    25201 gccaggcagc aaagagtgcg ccgcccgcgg tgatcagcca aacctggtta ccgtcccagt
    25261 gtggtgcaat ggagttaatc ataattcgac gctcggtgtc gttacgaccg aggaaacggg
    25321 tgagcatgcc cacccccatg tcgaaaccgt cagtgactgc aaaaccaatc agcagaacgc
    25381 caaccagcag ccaccagata aaacgcaata cttcataatc gatcatttga cgactcctgt
    25441 cttagcgtgc cggctgagta gtcgtggaag actgctcaaa gtgatagcga ccggttttca
    25501 ggctgcttgg gccgaggcgt gcaaatttga acattaagaa caattctgcc accaggaaca
    25561 gggtatacag gccgcaaatc agcaccattg agaagatgag atcgcctgcg gtcagtgacg
    25621 aattcgccac agctgtcggc agcacttcac cgatagccca cggttggcgg ccatattcag
    25681 ccacgaacca gcccgcttct acagcaatcc acggcagcgg aataccgtac agcgcggcgc
    25741 gcagcagcca ttttttctcg ccaatgcggt tgcggatgac actccagaaa gagagcgcga
    25801 tgattgccag cagcaggaag ccacacgcca ccataatacg gaacgcgaag tacagcggcg
    25861 ctacacgcgg aatggagtct ttagttgcct gttgaatctg cgcttcagtc gcatcagcca
    25921 cgtttggcgt atagcgtttc agcagcagac cgtaaccgag gtctttcttc atgctattga
    25981 actggtcacg aaccgcctgg tcggtagaac cagaacgcag ttgttcgagc agagagtacg
    26041 ccttcatccc gttacgaatg cgttcttcat gctgcaccat cagctctttc aggccgataa
    26101 ccggggtatc cacggaacgc gttgcaatga tgcccagtgc gtaagggatc tgaatcgcaa
    26161 atttgttcgt ctcctcttcc tgatcaggaa tgccgaacag agtaaaggca gcaggcgcag
    26221 gttgcgtttc ccactcggct tcaatagcag ccagtttggt tttctgcacg tcgcccattt
    26281 cgtagccgga ttcatcaccc agaacaataa cagacagaac agcagccata ccgaagctgg
    26341 cagcgatagc aaaggagcgt ttagcgaagg cgaagtcacg acctttcagc atataccatg
    26401 cgctgatacc gaggatgaac atcgcgccag tcacataacc agacgctaca gtgtgaacga
    26461 atttcacctg agcaaccggg ttaagcacca gctcggagaa gctcaccatc tccatacgca
    26521 tagtttcaaa gttgaaatcg gacgcgattg ggttttgcat ccagccgttc gcaaccagaa
    26581 tccacagtgc ggacaggttt gaaccgagcg ccaccagcca ggtgacacac atatgctgaa
    26641 ctttacccag acgatcccaa ccgaagaaga acagacctac aaaggtggat tcgaggaaga
    26701 aggccatcag accttcgatt gccagcggcg caccgaagat atcccctaca tagtgggaat
    26761 agtaagacca gttagtcccg aactggaact ccatggtcag accggtagcc acacccagag
    26821 cgaagttgat accaaacaac ttgccccaga acttggtcat atctttataa atctgtttgc
    26881 cggagaggac gtagaccgtt tccataatgg ccagcaggaa cgccataccg agcgtcagtg
    26941 gcacaaaaag gaagtggtac atcgcggtca aggcaaactg taagcgcgac agttcgacta
    27001 tatctaacat catgactcct tgctcatcgc atgaagactc cgagagtgaa ccccgttaga
    27061 aagggtcaca cgcatgcccc aatagaaatt tgtttgaaca ctttcatcat accatgtgaa
    27121 tgcacacaaa aacggcgtaa agaaaggttg caaacatgtt aataaaaact caaattgatc
    27181 ccacgtatat attacgccgc aaaatcctta caataaacag gtttttgttg aagcaaattt
    27241 gcatttttcg acggtgatca atttatagct aaattaccgc ctttcagcca atttgatcga
    27301 gaacaattta tctctttttg atgcccattt ccaagactta tacattgata aatatcaatt
    27361 cctacaaatt atgttaatca tgtttcaata aggtaacttt agtgacattt atgtttaaaa
    27421 tgtgtgagtt ataaggctga aattagtttc ggttggcaat taaagataaa aagagacgaa
    27481 gcaaaaacag cacggttgac cttaattctt tttttggtca ctggctaata cgacgcagag
    27541 tttcaggatt actaataaag gcataaatcc acccccgccg atattaacat ttaattaact
    27601 tataattata ttataattaa ctcacccagg gttattattt tgttaaaaat aaagccacat
    27661 atacgtggct tgagagtgaa gataaacgca aaattacctg atgcgctacg cttgccagtg
    27721 tctacatcat ctctgcaata tattgaattt gcctgctttt gtagaccgaa taaagcgttc
    27781 atgccgtatc cggcatgaac aaagcgccct ttgtcaacaa tctggccgcg catatgtggc
    27841 ttcggtttga ttgctattca ggcaagccgg taactgaacg tccgtgactg gccgggtaaa
    27901 aaaggccccg atagatttga accttgattt tcctcggtgg taatgtgttc atccatcatc
    27961 gtttctgagc aagaaatcac ctcgcgactg aaagcaacag tcgcatcaca ggttgctgat
    28021 tcagccggat taaacagccg taaaatcact tcttgtcggt cttcagcttt cttaagtgcg
    28081 cttatcaggc atcccactgg gggcattttc aacaaactat aactttccgg cacgttgaat
    28141 ccggctttgt tgagcttcat cgcatcccat gggattttgt tgtaacactg tactggagtc
    28201 agccatgctc gcgcctgctg agctacacca gcggcggttg gcgtaccggt ataactcaat
    28261 aaacttaggc gacaagaaag cagaccacgt agttgtgagt ccgggactgg cattttaatt
    28321 cccgaaggcc gcccaggcct taaaagcaga tcttctttgc ccagtaagcc cacgccacgc
    28381 agcaacgtaa tggcaaaggt tttcttctct tcaccgatga cttcaaattc acgtaaccct
    28441 tcgctaaaga cagccatgcc gttacgccct tcctgtaagg caacatagtt gagcatattc
    28501 cataccggaa ccggcgcttc tttccagcct tcttgctgcc agttgttcat tgcactgtcg
    28561 ttcacggggc gcgttagcga accaaactgc gtatctgcca gaacactgtc ggtgttaaaa
    28621 ggtgtaggga ccaggacacg aaggcgatga tcgtcagcct ggttatcaag gttgatatcc
    28681 acatcaatac gcctgctgtt atgactaaga gtgacaacca acaccacccc tactctgcca
    28741 gtggattgcc gggcgctgcg ttctgacaaa ttgagcggca ctgccatgtc atagcggata
    28801 acagccctgc tctgccaggc ttcatgaata atatcgcatt gcggtttcgc gttcgctgcg
    28861 gtaattaccc actcttcttt tgcgggtgaa tagtcatatt catcaccatc atcagagctt
    28921 tcttcaat
//
LOCUS       DAJTSL010000036        28663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTSL010000036 DAJTSL010000000
VERSION     DAJTSL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.036"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..925
                     /locus_tag="OO433_001426"
     CDS             <1..925
                     /locus_tag="OO433_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR6049954.1"
                     /translation="RRQDFLTFFGNREPCLIGMEACGGSQHWARELTKLGHKVRLLQA
                     RFVKAFVMGNKNDVMDARAIWMAVQQPGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQ
                     INALHGTLLEFGETIHKGRAAMEREFPEALERMKERLPPYLIMVLENQYNRLNELDSL
                     IEDIEKQLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFKSGREFAAYVGLV
                     PKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKKRRPASVAI
                     VAMANKLARTVWAITAHDRKYDRNHVSIRPY"
     gene            1636..4821
                     /gene="rne"
                     /locus_tag="OO433_001427"
     CDS             1636..4821
                     /gene="rne"
                     /locus_tag="OO433_001427"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6049955.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPHHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(5018..5971)
                     /gene="flgL"
                     /locus_tag="OO433_001428"
     CDS             complement(5018..5971)
                     /gene="flgL"
                     /locus_tag="OO433_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR6049956.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFAIQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLAVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(5983..7626)
                     /gene="flgK"
                     /locus_tag="OO433_001429"
     CDS             complement(5983..7626)
                     /gene="flgK"
                     /locus_tag="OO433_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR6049957.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(7692..8633)
                     /gene="flgJ"
                     /locus_tag="OO433_001430"
                     /pseudo
     CDS             complement(7692..8633)
                     /gene="flgJ"
                     /locus_tag="OO433_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
     gene            complement(8633..9730)
                     /gene="flgI"
                     /locus_tag="OO433_001431"
     CDS             complement(8633..9730)
                     /gene="flgI"
                     /locus_tag="OO433_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6049958.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVSGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSARLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(9742..10440)
                     /gene="flgH"
                     /locus_tag="OO433_001432"
     CDS             complement(9742..10440)
                     /gene="flgH"
                     /locus_tag="OO433_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6049959.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANVSNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVVDARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(10493..11275)
                     /gene="flgG"
                     /locus_tag="OO433_001433"
     CDS             complement(10493..11275)
                     /gene="flgG"
                     /locus_tag="OO433_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6049960.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMQQKLTQL"
     gene            complement(11413..12168)
                     /gene="flgF"
                     /locus_tag="OO433_001434"
     CDS             complement(11413..12168)
                     /gene="flgF"
                     /locus_tag="OO433_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR6049961.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKIDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(12188..13396)
                     /gene="flgE"
                     /locus_tag="OO433_001435"
     CDS             complement(12188..13396)
                     /gene="flgE"
                     /locus_tag="OO433_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6049962.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATPQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(13421..14116)
                     /gene="flgD"
                     /locus_tag="OO433_001436"
     CDS             complement(13421..14116)
                     /gene="flgD"
                     /locus_tag="OO433_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6049963.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTMLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAITASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(14128..14532)
                     /gene="flgC"
                     /locus_tag="OO433_001437"
     CDS             complement(14128..14532)
                     /gene="flgC"
                     /locus_tag="OO433_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR6049964.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(14536..14865)
                     /gene="flgB"
                     /locus_tag="OO433_001438"
     CDS             complement(14536..14865)
                     /gene="flgB"
                     /locus_tag="OO433_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6049965.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTLGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYQMSLSALSGQIKGMM
                     NVLQSGN"
     gene            15021..15680
                     /gene="flgA"
                     /locus_tag="OO433_001439"
     CDS             15021..15680
                     /gene="flgA"
                     /locus_tag="OO433_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6049966.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARMRMVSGLVVSGVVDADGNILIN
                     L"
     gene            15756..16097
                     /gene="flgM"
                     /locus_tag="OO433_001440"
     CDS             15756..16097
                     /gene="flgM"
                     /locus_tag="OO433_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6049967.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTDSTSTSTSTS
                     TSTSTSTSTSTSVTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIADAL
                     INEAQQDLQSN"
     gene            16102..16518
                     /gene="flgN"
                     /locus_tag="OO433_001441"
     CDS             16102..16518
                     /gene="flgN"
                     /locus_tag="OO433_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6049968.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(16558..18093)
                     /gene="murJ"
                     /locus_tag="OO433_001442"
     CDS             complement(16558..18093)
                     /gene="murJ"
                     /locus_tag="OO433_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6049969.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(18260..19126)
                     /locus_tag="OO433_001443"
     CDS             complement(18260..19126)
                     /locus_tag="OO433_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6049970.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(19128..19775)
                     /locus_tag="OO433_001444"
     CDS             complement(19128..19775)
                     /locus_tag="OO433_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6049971.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAELESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(19786..20370)
                     /gene="rimJ"
                     /locus_tag="OO433_001445"
     CDS             complement(19786..20370)
                     /gene="rimJ"
                     /locus_tag="OO433_001445"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6049972.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            20606..21814
                     /gene="mdtH"
                     /locus_tag="OO433_001446"
     CDS             20606..21814
                     /gene="mdtH"
                     /locus_tag="OO433_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6049973.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            21878..22525
                     /gene="grxB"
                     /locus_tag="OO433_001447"
     CDS             21878..22525
                     /gene="grxB"
                     /locus_tag="OO433_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6049974.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            22659..23219
                     /locus_tag="OO433_001448"
     CDS             22659..23219
                     /locus_tag="OO433_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6049975.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            23325..24371
                     /gene="pyrC"
                     /locus_tag="OO433_001449"
     CDS             23325..24371
                     /gene="pyrC"
                     /locus_tag="OO433_001449"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6049976.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREAHFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            24445..24690
                     /gene="dinI"
                     /locus_tag="OO433_001450"
     CDS             24445..24690
                     /gene="dinI"
                     /locus_tag="OO433_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6049977.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGVTKEDKQRISEILQETWESADDWFVSE"
     gene            24980..25234
                     /gene="bssS"
                     /locus_tag="OO433_001451"
     CDS             24980..25234
                     /gene="bssS"
                     /locus_tag="OO433_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6049978.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            25349..26467
                     /gene="solA"
                     /locus_tag="OO433_001452"
     CDS             25349..26467
                     /gene="solA"
                     /locus_tag="OO433_001452"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6049979.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCTGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            26515..26628
                     /locus_tag="OO433_001453"
     CDS             26515..26628
                     /locus_tag="OO433_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6049980.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            26889..27455
                     /locus_tag="OO433_001454"
     CDS             26889..27455
                     /locus_tag="OO433_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6049981.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFTLGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            27459..28034
                     /locus_tag="OO433_001455"
     CDS             27459..28034
                     /locus_tag="OO433_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6049982.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGNDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(28076..>28663)
                     /locus_tag="OO433_001456"
     CDS             complement(28076..>28663)
                     /locus_tag="OO433_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6049983.1"
                     /translation="RNHYEYEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMY
                     CTGGIRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGE
                     RISDEIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPP
                     EEQRRRRAGRENGNKIFNKSRGRLNTTLGIPDPTE"
BASE COUNT         6930 a         7586 c         7256 g         6891 t
ORIGIN      
        1 gcgtagacag gattttctga cgttcttcgg caaccgtgag ccatgcctga ttggtatgga
       61 ggcctgtgga ggttctcagc actgggcacg ggaactgaca aaacttggtc ataaagtccg
      121 gttgttgcag gcccgcttcg ttaaggcatt cgtcatgggc aataagaatg atgtgatgga
      181 tgcccgggct atctggatgg cggttcagca gccgggtaaa gaaatcgccg taaaaacaga
      241 agaacagcag tcggtactgg ttctgcaccg tacccgcatg caactggtga agttccggac
      301 cgcacaaatt aatgccctgc acgggacgtt actggagttt ggtgaaacca tccacaaagg
      361 ccgggcagcg atggagcggg agttccccga agcactggaa cggatgaaag agagactgcc
      421 accgtatctc attatggttc tggaaaacca gtacaaccga ctgaatgagc tggactcact
      481 gatagaggat attgaaaaac agcttaccag cgtggcgagg cagaatgaaa cctgtaagcg
      541 gttgctggat attcctggcg ttggaccact tattgcgacg gcagcggtgg ccaccatggg
      601 ggaagcatca gcgtttaaat cggggcgaga gttcgccgca tatgttggtc tggttccaaa
      661 acaaactggc tccggaggga aagtacgtct gctggggata agcaaacgtg gtgacactta
      721 tctcaggaca ttatttatcc acggtgcaag agcggtggca ttagtagcta aagagcctgg
      781 cccgtggata accgaactga aaaaacgtcg tccagccagt gtggcaatcg tcgccatggc
      841 aaacaagctg gcacgaacag tatgggcgat aaccgcccat gaccgtaagt atgacaggaa
      901 ccacgtcagt atcagaccat attaatcgct gataccatta aacaatgaac tcttaacaaa
      961 agggtgaatg ctgaaaggtt gctatggcgg ccagagtgat gacaaagaca ggtaagaccg
     1021 tgactcacta aacctgaaca gtattttggg cttgaagtcc gccgtgaaaa taaggggtga
     1081 gtcggcgaat tacatagggg ctcgcagcgt tacggctgca ataaagccgg atataaagct
     1141 gcaacctacc cgtcatgtca aaacaatgga tgccttgcaa acgggatgcg ttcatataaa
     1201 aactgtgagt aagcgggtga taaatggtaa aagtcatctt gctataacaa ggcttgcagt
     1261 ggaataatga agccgtttcc gtgtccatcc ttgttaaaac aagaaatttt acggaataac
     1321 ccattttgcc cgaccgatca tccacgcagc aatggcgtaa gacgtattga tctttcaggc
     1381 agttagcggg ctgcgggttg cagtccttac cggtagatgg aaatatttct ggagagtaat
     1441 acccagtctg tttctttgat aattgcgctg tttttccgca tgaaaaacgg gcaaccgaca
     1501 ctctgcgcct ctttgagctg acgataaccg tgaggttggc gacgcgacta gacacgaggc
     1561 catcggttca cacccggaaa ggcgttactt tgcccgcagc ttagtcgtca atgtaagaat
     1621 aatgagtaag ttacgatgaa aagaatgtta atcaacgcaa ctcagcagga agagttgcgc
     1681 gttgcccttg tagatgggca gcgtctgtat gacctggata tcgaaagtcc agggcacgag
     1741 cagaaaaagg caaacatcta caaaggtaaa atcacccgca ttgaaccgag tctggaagct
     1801 gcttttgttg attacggcgc tgaacgtcac ggtttcctcc cactaaaaga aattgcccgc
     1861 gaatatttcc ctgctaacta cagtgctcat ggtcgtccca acattaaaga tgtgttgcgt
     1921 gaaggtcagg aagtcattgt tcagatcgat aaagaagagc gcggcaacaa aggcgcggca
     1981 ttaaccacct ttatcagtct ggcgggtagc tatctggttc tgatgccgaa caacccgcgc
     2041 gcgggtggca tttctcgccg tatcgaaggc gacgaccgta ccgaattaaa agaagcactg
     2101 gcaagccttg aactgccgga aggcatgggg cttatcgtgc gcaccgctgg cgtcggcaaa
     2161 tctgctgagg cgctgcaatg ggatttaagc ttccgtctga aacactggga agccatcaaa
     2221 aaagccgctg aaagccgccc ggccccgttc ctgattcatc aggagagcaa cgtaatcgtt
     2281 cgcgctttcc gcgattactt acgccaggac atcggcgaaa tccttatcga taacccgaaa
     2341 gtgctcgaac tggcacgtca gcatatcgct gcattaggtc gcccggattt cagcagcaaa
     2401 atcaaactgt acaccggcga gatcccgctg ttcagccact accagatcga gtcgcagatc
     2461 gagtccgcct tccagcgtga agtccgtctg ccgtccggtg gttcgattgt tatcgacagt
     2521 accgaagcgt taactgccat cgacatcaac tccgcacgcg cgacccgcgg cggcgatatc
     2581 gaagaaaccg cgtttaacac caacctcgaa gctgccgatg agattgctcg tcagctgcgc
     2641 ctgcgtgacc tcggcggcct gattgttatc gacttcatcg acatgacgcc tgtacgccac
     2701 cagcgtgcgg tagaaaaccg tctgcgtgaa gcggtgcgtc aggaccgtgc gcgtattcaa
     2761 atcagccata tttctcgctt tggcctgctg gaaatgtccc gtcagcgcct gagcccatca
     2821 ctgggtgaat ccagtcatca cgtttgtccg cgttgttctg gtactggcac cgtgcgtgac
     2881 aacgaatcgc tgtcgctctc tattctgcgt ctgatcgaag aagaagcgct gaaagagaac
     2941 acccaggaag ttcacgccat tgttcctgta ccaatcgctt cttacctgct gaatgaaaaa
     3001 cgttctgcgg taaatgccat tgaaactcgt caggacggtg tgcgctgcgt gattgtgcca
     3061 aacgatcaga tggaaacccc gcactaccac gtgctgcgcg tgcgtaaagg ggaagaaacc
     3121 ccaaccttaa gctacatgct gccgaagctg catgaagaag cgatggcgct gccgtctgaa
     3181 gaagagttcg ctgaacgtaa gcgtccggaa caacctgcgc tggcaacctt tgccatgccg
     3241 gatgtgccgc cagcgccaac gccagctgaa cctgccgcgc ctgttgtagc tccagcaccg
     3301 aaagctgcac cggcaacacc agcagctcct gcacaacctg ggctgttgag ccgcttcttc
     3361 ggcgcactga aagcgctgtt cagcggtagt gaagaaacca aaccgaccga gcaaccagca
     3421 ccgaaagcag aagcgaaacc ggaacgtcaa caggatcgtc gcaagcctcg tcagaacaac
     3481 cgccgtgacc gtaatgagcg ccgcgacacc cgtagtgaac gtactgaagg cagcgataat
     3541 cgcgaagaaa accgtcgtaa tcgtcgccag gcacagcagc agactgccga gacgcgtgag
     3601 agccgtcagc aagctgaggt aacggaaaaa gcacgtacca ccgacgagca gcaagcgccg
     3661 cgtcgtgaac gtagccgccg ccgtaacgat gataaacgtc aggcgcaaca agaagcgaag
     3721 gcgctgaatg ttgaagagca atctgttcag gaaaccgaac aggaagaacg tgtacgtccg
     3781 gttcagccgc gtcgtaaaca gcgtcagctc aatcagaaag tgcgttacga gcaaagcgta
     3841 gccgaagaag cggtagtcgc accggtggtt gaagaaactg ccgctgccga accaattgtt
     3901 caggaagctc cagctccacg cacagaactg gtgaaagtcc cgctgccagt cgtagcgcaa
     3961 actgcaccag aacagcaaga agagaacaat gctgataacc gtgacaacgg tggcatgccg
     4021 cgtcgttctc gccgctcgcc tcgtcacctg cgcgtgagtg gtcagcgtcg tcgtcgctat
     4081 cgtgacgagc gttatccaac ccagtcgcca atgccgttga ccgtagcgtg cgcgtctccg
     4141 gaactggcct ctggcaaagt ctggatccgc tatccaattg tacgtccgca agatgtacaa
     4201 gttgaagagc agcgcgaaca ggaagaagta caagtgcagc cgatggtgac tgaggtccct
     4261 gtcgccgccg ctgtcgaacc ggttgttagc gcgccagttg ttgaagaaat ggctgaagtc
     4321 gtagaagccc ccgtaccggt tgccgaaccg caaccggaag tggttgaaac gacgcatcct
     4381 gaagtgattg ccgccgcggt aactgaacag ccgcaggtga ttaccgagtc tgatgttgcc
     4441 gtagcccagg aagttgcaga acacgcagaa ccggtggttg aaccgcagga agagacggca
     4501 gatattgaag aagttgccga aactgctgag gttgtggttg cagaacctga agttgttgct
     4561 caacctgccg cgccagtcgt cgctgaagtc gcagcagaag ttgaaacggt agccgcggtt
     4621 gaaccagaga tcaccgttga gcataaccac gctaccgcgc caatgacgcg cgctccggca
     4681 ccggaatatg ttccggaggc accgcatcac agtgactggc agcgccctac ttttgccttc
     4741 gaaggtaaag gtgccgcagg tggtcatacg gcaacgcatc atgcctctgc cgctcctgcg
     4801 cgtccgcaac ctgttgagta ataattagct caaagtaatc aagccctggt aactgccagg
     4861 gcttttttat ttcatgtttg aatcaacata ttaaaattca tcaaatggca tccttgctaa
     4921 ccaacaatgc aaaataggca aaaaaaatgc ccgccccggc gatccctgag cgggcagaca
     4981 aaacataccc agtttcatga tatgttttaa agcgagctta tttgttgagc tggaagagcg
     5041 acaatccctg catatcggta aatgctttat acgatgcctg caatgccgtt tgctgcatga
     5101 tgtaagatga aatagttgca ttccagtcca catcaaccag atcgctcatc tgctgcgttt
     5161 gccctaaagc gcgatcgcta cctaaggaat ccagcgactc cagttcgttc agctgcgtgc
     5221 ctaattccgc gcgaacggca aggacattat tcagcgagtt tttcagtcca cggttggttt
     5281 tatccaatgc cgcagcggcg atttctttat ccgcttcgct atccgcgacc ggcgttttca
     5341 gggctgcgat ggcgctatcc agcatggcaa aaagattggt ttcagaagcg ctaccgtctg
     5401 gctccgcaac tgcgttgctg gtaatactgt cgaaaatttt gtcacccgtg tgccctatca
     5461 ccatcgaacg cgaagcatcg acctgttgct taatactttc tgctccaccg acgtatttcc
     5521 ctttctcttc gctaaacggc gcagtctctg ttttataacc ggcaaaaatg tagcgcccgt
     5581 taccgtcagt ggtgttcgcc agattcagca actggtcacg aagcccctga atatccgtag
     5641 ccagcgaggc ccggtcatcg tcactcaagg tgccattgct ggcgtagaca attttttcct
     5701 gagcattctg gatagcagtg gtgacctggc taagtacact ctcttccagt gacacttttt
     5761 gaatggcgaa agtacgcgcc agcgtgtact ggctgttttg cgcctgtgcc tgggagagaa
     5821 ctacggcttg tgatgctgca atgggatcgt cagaagggtt aacgactcgc ttacctgtcg
     5881 acatctgctc gccgtacttc atccattccg cctgagaatt ggtgatgcca cgcatgtttt
     5941 gctggtacat catctgggta ctgaaacgca tcttatctcc ccttagcgaa tgttaatcag
     6001 cgcatcaaaa atcgcgtttg ccgtctgcag aacctgcgca ttcgccaggt aatactgctg
     6061 aaaacgttgc agatttccgt actcttcgtc gagattgacg ccagagatag actgctgctg
     6121 attggaaagc tgcgtcacca cattaccttg cgtggcgcta ctggttttca acgtcgcggt
     6181 tttattaccg atatcactca ctaacgaggc ataagcgtcg ttaaaggatt tcgcaccgcc
     6241 caccgtttta ctgttgcttt gcagatccag cagggcctga ccgttgcggt tatcgctatc
     6301 acccgcatct ttttcgctcg ccatcgctat tttcgcttcg tcggtgatta atacatccat
     6361 gttgacgatg gcgtcactta ctggtttcag cgtgaagctg tcgttaacgg caggcgttcc
     6421 tgtaaacgtc aactccagac catcaaatgc cactttaccg ttggcatccg gcgtcaccgt
     6481 aaaagtggta ttgctggcaa ggcgggtgac ctgccactga ttattatcga acgagatttt
     6541 gtaatctgtc gccagtaccg cggaggcatc agttaccgtg gcaccgatcg caacgtcacc
     6601 tttgtttttc gtgttttgca aaaccgcggg cttaccgata gcaaagaaat cttcaccagc
     6661 atcgccgttg gcatcaaatc cggctttgtg ttgggtgttg aaagcctcgg caaatgccag
     6721 cgccagttgt ccaagcgtat tacgcgtctg gtccagatcc tgagaacgga atgtcagaat
     6781 gccgcccagc gacccggtat tcagtaattt ctccgggatc tcaatattgc ctgccgtccc
     6841 atcaacgtaa gcgacagtcg tacgagaagg gtcggcgctg gaaggaactg ccgccagttg
     6901 ccgcgccgta cttccctgaa ccagtgagta accattggcc atcgtgatgt tataagtgcc
     6961 gccatcctga acgctgactt ctacaccaac aatctggttt aattcgctca ccagttgatc
     7021 gcgttgatcc agcagattgt taggtgacgc ccctgccccc acgcctgtca ggcgggagat
     7081 ttggtcgttc aggctggcga tttgtttagc gtaattgttg atctgatcaa cgctggcacc
     7141 tatcgcgata ttgacctgtt tgtcctggtc acgaagatac tgatcggtgg ttttaaactg
     7201 attcaccaac ccttctgatt tcccaatcag cgcctggcgc gctgccgggt cttccgcgtt
     7261 actcaccagc gtttgcaggc tggtgaagaa atcctgcatc tgtgttgcca gcgaagaggt
     7321 actggtggag agcatattgt cgattttcga catctgctca tagcgggcag tcaggccgct
     7381 actttgcgtc tgcgccgcac gtaactggtt ggtgataaac gcatcatact cacgctgcac
     7441 accagaaacg tagacaccat tgccaaccca gccgccagcg cccaacgtgc tattggcctg
     7501 cgccataata gtggtttggc gggtatatcc ggcaacgtta tagctggaga tattattact
     7561 tgccgtattt aacgccgcct gggccgcgtt cagtccgctc atggcgttat taatcaagct
     7621 ggacatggag gttccttata agccttcaat tacagagtat tatcggcagc gacccgccgg
     7681 acttgagtta ttcaaaacag attatcaatg ttcatactgt aggttttgct caccttgtcg
     7741 cttatcgatt tcatctgctg aatcatgttg gtgagtttgc gggcatagtg aggatcggtg
     7801 gcatagcccg cgtcctgtag ggcctgcgcc ccctgctccg cactcgtggc ggtggtcacg
     7861 gcggcgtagc gcgggttacg cgttaacagc ccaacgtaat ccgactatgc ttccagatac
     7921 gagctgtaga cccgaaactt cgcttttact ttcttcgctt cgccgttttc atattcagtc
     7981 gtggtgattt cagtaactgg ccctttccag ttgccagagg ctttgacacc aaacaggtta
     8041 tagctcggct cgccgttttc gcggcggatt tgccgttgcc cccaaccaga ttccagcgcc
     8101 gcctgagcga ggatcaaatg atgtggcaca ccgctttgct ggcttgccag ttgggcgggc
     8161 agcgagagtt gcgcgaggaa tgctttactg tcacccggca gcgaatcatc gtagttacgt
     8221 ggcacggcct tttgcaccag ctgcgaaagc gcctgatttt gataacgcac cacagtttcg
     8281 agcgggaatt tcatcggtgc tgctggcgtg gactcctctg gcaatggttg ttctggcgtc
     8341 atctgtttaa ccatcatctc tgcaagcccc agacctttgc ccgccgtcat ctgttgggca
     8401 atctgctggt catacatact ggtatacagg cgagtgtgct cgctgctgaa caggccatct
     8461 tttggtaaag cgtcgcgcat gcttttcaac atcatctgca cgaacatccc ttccacctga
     8521 cgggccaccg gacggatatt tgccgccgga tcttcgcccg ctttcgcctt tagttcgttg
     8581 agtgattgcg catcccaggc cgcacttgcc agtagtttgc tatcgctgat catcagatga
     8641 tttccagttt tgcccgcaga catcccgcac tttgcattga ttgcagtatg gacatcagat
     8701 ccatcggcgt agcgcccagc gcattgagcg cgcgcaccac gttattgagc ctggcgctgg
     8761 aacgtacgct ttgcagcgaa ccgccgctct ggcgtaaatc gatctgcgtt tgtggagtaa
     8821 ccacagtctg tccaccacca aacggtgtat ctggctggct gacattggcc tgacgattaa
     8881 ctgttactga gagattcccc tgcgctaccg cgcagctgtc gagggtcact tcgcgattca
     8941 tcaccaccga accggtgcgc gagttaatca ctactttagc gtcctgcggg gtgacattaa
     9001 cctgcatatt ctggatatcg gcaaggaagc ggacctggga actgttgcca ctcggtacgc
     9061 gcacctgaat agtccgcgca tctaacgcgg tggcgctgcc atatccacgc acgcggttga
     9121 tggtgtcagc gatttgctgc gccatgctga aatcttcgtc gttaagttgc aaattaaggg
     9181 tattcccgac gccaaactgg ctgggcaatt cacgttcaat aaccgcacca ttggtgatcc
     9241 gtccaccgtt cagttggtta acctgaacac tgctaccgcc agcggaggct cctgcgccgc
     9301 taaccagaat attgccctgc gccagcgcat acacctgact gtcaacgccc ttaagcggtg
     9361 tcatcaacaa cgtacctcca cgcaagcttt tggcattacc catggaagaa accaccacat
     9421 cgatggtttg cccctgacgt ccaaacggag gaagtgacgc tgtcaccatt accgcagcga
     9481 cgttttttag ctgcatattg gtgcccgtcg gaacggtaat tcccagctgt gagagcatgt
     9541 tattaagcgt ttgtgtggta aacggcgtct gggttgtctg gtcaccggtg ccatccagcc
     9601 ccaccaccag accatagcca atcagtgagt tttgccttac cccctgaaca ctggtgagat
     9661 cgcgaatacg ctcagcctga gccgccgtcg tgaccagtag aagaattaat gcagagagaa
     9721 atttaatcac cacagcctca cttacattgg cgacaggtta aggaagaaac gctgcaacca
     9781 gcccatattt tgcgcttcgt taatgtagcc attgcctacg tattcaatgc gcgcatccac
     9841 cacctgagta gacggtacgg tattgctgcc gctgatagtg cgtggattaa ccacgccaga
     9901 gaagcgaata aattcggtac cctgattaat ggcaatctgt ttttcaccca ccacatgcag
     9961 gttgccgttg accagtacct ggtcaaccgt caccgtcaac gtgccgctaa aggtattgct
    10021 gacattggcc ccgccctttc cgttgaacgt gttaccaccg gaggcttcga catcggcacg
    10081 agcgttacca aacagcccct gcaaatagcg cggcacagta tcaaagccaa aattagtttt
    10141 accgtcacgg ctggcattcg cagaggagct tttgctggcg ctgacgttct cctgcaacac
    10201 gatggtcagc gtatcgccaa tattgcgtgg tcgacgatct tcaaacagcg gttgatagcc
    10261 atagttaatc ggctgagcag actggaaaat agaaccgttg gcgacgggcg tcggaccggg
    10321 aaccggttgt gcactggtcg ccccctgcac cagcggcgtg gagggtatcc aggcgcagcc
    10381 ggttagtgaa agcaccaaca agctggaaat ggcataagta tgcgcagcgt ttttttgcat
    10441 ggctatcatc ttcaaaaatc agtaaaccgg tgaacctgcc accggttaag ccttagagtt
    10501 gcgtcagttt ttgctgcatc tgatcggtgg tggacaccgc tttactgttg atttcgtaag
    10561 cgcgttgcac ctgaatcata ttgaccagtt cttccgccac gttgacgtta gacgtttcaa
    10621 catacccttg atacagcaat cccgcgccgt tcaggcccgg cgtgctttcg ttcggtgcac
    10681 cagaggattg cgtttcggtg tagaggtttt cgccaatgct ctccagcccg gtgtcattca
    10741 taaaggtggt gagattgagc tgcccaacct gaaccggagc tgcctggcct tgttgggtta
    10801 cgctgaccac gccatcacga ccgatggtga tacttaacgc attcgccgga atggtgatcg
    10861 ctggctgcac ctgaaaacca ccagccgtca ccagctgccc gttctgatcc acctggaaag
    10921 agccgtcacg ggtataggct gatgaaccat ctggcaacat cacctggaaa aagccctgcc
    10981 ctttaatcgc gacatctttg ctgttgttgg tctgcgacag atttccctgg ctgtgtaagc
    11041 gttcagtggc gaccgggcgt acccccgtgc cgatttgtaa tccggagggt aaggtggttt
    11101 gttcggaaga ctgtgccccc ggctggcgaa tggtttgata aagcagatct tcaaacaccg
    11161 cgcgctgacg cttaaaaccg ttagtactga cgtttgccag gttgttggca atgacgtcca
    11221 tattggtttg ctgggcgtca aggcccgttt tggcgatcca taatgaactg atcataaaat
    11281 gtcctgtagt ggattagtga caagcagagc agaagcacgc gacagtgtga attccccctc
    11341 actctaaccc tctccccaaa ggggcgaggg aatagtccgc gcgcttttat tgcaatgtca
    11401 tcaatcgttc aattaactca tcgacagcag ttggttggca cggcctgcgt tatcatcgac
    11461 gctgctgatc accttcatct gcatttcaaa acgccgcgcg ctggcaatca tgtcgctcat
    11521 tgccgcaacg gcattgacgt tactgccttc cagaaccccc gacatcacac gcaaggttgg
    11581 atctgcctgc agtaccggcc ctcgcgtggc ctgggtttct gcgcttaaac gaaaaatgcc
    11641 gtcgtcaccg cgctgcactt cgctgcccgt ggctttcacc agtttcagac gccctactgg
    11701 cgcaactgta tttgccggat cgcccggatt gagcgccgag attgtgccat cggcagcaat
    11761 agtgatttcc gctccttccg gcacagcaat tggccccgcc tcgcctatca cctgatgccc
    11821 ctgaattgtc agttgcccgg tgggatcaac ctgaatgctg ccattacgcg tatacccttc
    11881 gctgccgtca gcggtctgca cggccagcca gccatcctgc tgcaacgcaa cgtccagcgg
    11941 gcgcgaggtg taatctatct tgccgggcgt catgtctgca cccggtgttg acgccgttac
    12001 cagcgtgcgc gtgggcagtg aaagcccttc caccggcact gcgcgcaagg cgtttaactg
    12061 cgcgcgaaaa ccgggcgtag aggcattggc cagattactg gcggttaccg cctgttgatt
    12121 cagcgtctgg ctggctgctc ccatcgcagt ataaattgcg tgatccattg agctatcccg
    12181 tcagcgatta gcgtaagtta accagcgtgt tgaggatctg gtcctgggtt ttgatggtct
    12241 gggcgttaga ctgatagtta cgctgggcaa cgatcatatt gaccagttct ttactgagat
    12301 cgacgttgga cgcttccagc gcaccgttgg tcagggtgcc aaagtttccc gtcccggctg
    12361 tccccaacag cgccacgcca gaagattgcg tcgcagacca gacgttgtcg ccttcggatg
    12421 ccagaccttc gttgttggca aagttcgcca gtacaatctg ccccagcagt tgagtttgtt
    12481 cgttggaata gttgccgaca accgtaccgt catcattgat ttgataactc accagatcgc
    12541 ccggtttgta gccgttctgg ggggttgcca caatattgtt agcgccggta ttttgctgca
    12601 tggagttgag gaagctcaga ctaaacgtgg cgggttctgc accgttaatt gcgccggttg
    12661 cgatattatt cgccatcgca ccatccacta atgtgccatt agcattaaat tccagtgttg
    12721 tcgctgtctt cgcaatgctg tttggatcac tgctatcctg ggtgtagacc tgccagttat
    12781 tatccccggt cttcacaaag tagacgctca tgtcatgagc attaccctga ctgtcgaaaa
    12841 cagtcaccga accttttttg ttatagctat ccgcattgct ggcgctgaat ggcgtaacag
    12901 taggaagcgg atcactggaa ttcaggttga tctgcatcga cgccgtggtg gtagttttcg
    12961 ctgccatcag ggtattcggg atcgaaatat tggtcggatt cgccccttgc tgaatagtcg
    13021 gcggcgtacc ggttgccggg taacccgtca gctgtaaacc ttgcatattc accaggttac
    13081 ggttttcatc cagcttaaat tgtccgttac ggctgtagaa caccgaaccg ttgctgtcta
    13141 ccagacggaa aaaaccgttc tggctgatag caacgtccag acctcgcccg gtgttggtgg
    13201 tcgtgccatc tgtaaagtcc tgagtgatac cggcaacttt tacccccagt cccactttcg
    13261 aaccggcaaa catatcggca aaagaggccg tgcctgattt aaagccgtag gtggcggagt
    13321 tggcaatatt gttgccaata acatcgaggt tagtggcggc agcgtttaat ccgctaaccg
    13381 cttgagaaaa ggccatgact gactcctgaa atgtgaaggc ttaaattatc tgccgtactt
    13441 cgtcgagggt ggtggtgccg taagtgccga gatccagcgt attaccgctg ttgccgcgga
    13501 tcacaccctg caccagagca aactgcagcg gctgggcaac cagttgtgta ccaccgttac
    13561 tggcggtaat cgctacattg taagaaccgt tcggcgcagt gctgccatca gtcaacgtac
    13621 cgtcccaggt gaaactgtga actccggcgg tcagttcacc aatatcaatg gtgcgcacaa
    13681 ccgcgccatt tttatcggtg atggtggccg taactttgtc tgccgcctgt tgcagctcaa
    13741 caccaaacgg cgtggtcgtg gtcacagccc cttcttcact gccggttccc gcaagaacag
    13801 ttgtgccggg gatcatcacg ccgtgaccga tcaggttact ggcctgtaac gactggctgt
    13861 tatcaatctg tccggaaata gatccgagca tggtattgag tttttcaatc ccgctgaccg
    13921 tgctgatttg tgccaattgc gacgtcagct cgttgttttc cattggattg gtcgggtcct
    13981 ggtttttcag ctgcgccacc agcaaagtca gaaaactgct ttgtaaatct gcggcgttgc
    14041 tgcccgtgag cgaactactg ctggtggtac tgacgccggt atttgtcgga tcggtggtgg
    14101 ttaccgcaat ggacatagct ttctccttta ttgaccgagc gtaagggttt tcagcatcat
    14161 gcttttcacc gtgttgagca cttcaacatt ggcctgatag ctgcgtgacg ccgacatggt
    14221 gttaaccatc tctccgacaa catcaacgtt cggcattttt acgtagccct ttgcatccgc
    14281 cagcggatta cccggttcat aaaccagttt gtccggggcc tgactttcta taacatcggc
    14341 aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta acctggaata ccacctgttt
    14401 tgcccgatat ggctgtccat cgggaccggt cacgctatca gcattcgcca gattactggc
    14461 cgccacgttc aggcgctggg actgggcagt taacgccgac ccggcgatat caaaaatatt
    14521 cagcagtgcc atccgttaat ttccgctctg taaaacgttc atcatgcctt tgatttgccc
    14581 gctcaacgca ctaaggctca tctggtattg cagttctgcg gtaggaggcg tcagcgcctg
    14641 cgccggaatg tgttgcgttg aggtcatcgt cagagcaacc acactggttg catcccgtcc
    14701 acgttgcatg acttttttaa gttcactggc aaaatcgata tcgcgcgcct gataaccaag
    14761 ggtatcggca ttggcgatgt ttgctgccag cacttcctga cgctgggcgc gcagattgag
    14821 cgcctcttgt tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt
    14881 atcaaaattc tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc
    14941 aaaatgggtc gctatttatg ccgttgatga ccattgggga cgggtacaat tcacgttgta
    15001 gaaatagctg gggggcgaaa atgctggcaa taaaacgtag cgtggcgatc atcgcgatac
    15061 tgttcagtcc gttaagtgcg gcgagcaatc tcacatcgca attgcacacc ttttttagcg
    15121 cccagctcgt gggggtaagt gatgaggttc gtgtttctat tcgtacagca cccaatctac
    15181 taccgccgtg cgagcagcca ttgctttcga tgagcaataa ttcccgcctg tggggcaatg
    15241 tgaatgtgtt ggcacgctgc ggtaacgaca aacgatattt acaggttaat gtgcaggcca
    15301 caggaaatta tgtggttgcc gcgatgccca ttgtgcgggg aggaaagctg gaagctggca
    15361 atgtcaaact aaaacgcgga cgactggata ccctgccacc gcgtacggtg ctggatatca
    15421 atcaacttgt tgatgccatt agcctacgcg atctatcacc cgatcaacca atccaattaa
    15481 cccattttcg ccaggcatgg cgggtaaaag cgggacaacg cgtcaacgtg atcgccagcg
    15541 gtgacgggtt tagcgccaac gcagaaggtc aggcgctgaa caatgcagcc gtcgcacaga
    15601 atgcgcggat gcgcatggta tcgggactgg tggtcagcgg cgttgttgat gcagatggga
    15661 atattcttat aaacctgtaa gctgttaaag attacccgtc ccttgccgat aaataagcaa
    15721 cacatgataa aagcgccctc aatgaggaat aaaccatgag tattgatcgc acttcgcctc
    15781 tgaatcccgt aagcaccgtt caaccgcgcg aaaccactga cgcgccggta acgaacaccc
    15841 gggcggcaaa aacaaccgac tccaccagca ccagcaccag caccagcacc agcaccagca
    15901 ccagcaccag caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac
    15961 ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg
    16021 gtgaactaaa aatggacatc ggcaaaattg ccgatgcgct gatcaacgaa gctcagcaag
    16081 acttgcagag taactgatcg tatgacacgt cttgcagaga tccttgacca gatgtccgct
    16141 gtgcttaacg atctcaaaac ggtaatggat caagagcagc aacatctctc tatggggcag
    16201 atcaacggca gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg
    16261 gattacctcg aacagttacg caagaaagaa cccaatacag caaatagcgt tgatattagt
    16321 caacgctggc aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac
    16381 ggttggttac tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg
    16441 catcaggaac cgacgctata tggggcgaac ggtcagacct caacaaccca tcgcggcggt
    16501 aaaaagattt cgatctgaag attatctccg gcctgcactg caggccggaa tgcattgtta
    16561 caccgtccgg cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta
    16621 cgcggcaatc cccgccagca cgaccgccat taaacgcagt aagcgccagg gcatggtacc
    16681 caatgaccac tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac
    16741 cgccaccacc agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg
    16801 cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag
    16861 tgacagcccg gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat
    16921 cagcgtaacg atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg
    16981 agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg
    17041 ctgggtcatc agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa
    17101 cggacctgaa agaatgccca acgcaaccgc actcggcagc gccaacagga aacaaagacg
    17161 caacccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga
    17221 cagcgacggc agcaaaatgg tgccaagcgc cacgcccagc acaccggacg gaaactccat
    17281 taagcggtcg gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat
    17341 ggtgttgatg attaaggaga tctggctcac agagacgcca aggatcgccg gtcccatctg
    17401 tttcaccacg cgcattgctc cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc
    17461 gatcttcttc aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc
    17521 ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat
    17581 catgctgatg ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt
    17641 cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt
    17701 tagtagctgg ctggtcaggg caaatttgtc agctgtatca gcgaagcctg gcgcggtcac
    17761 catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt
    17821 cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt
    17881 atattccgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg
    17941 taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa
    18001 gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga
    18061 gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccaga ccaacgggca
    18121 aaacgcctgc aagacaggcg ctgaaagagg cgaatagtct acctggatta tggtgaattg
    18181 ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga
    18241 tacgttgcgc cagcacggcc tattcgccgg cggtttgcgg aactgtctga ttttgcacac
    18301 attcaatgaa gtgccgcgca cagccgacaa atccacgttg ttcaagagtg ctctgccaac
    18361 caggaatcgg tttatgcact acgccctgcc cgcgctcctc acgccattcg cgcatatccg
    18421 taatgtcgat gagcgcaccg tcagtcacgg cctgcacggt ttcacgctga cttccggcac
    18481 ggcgatgcat acaggtggtg atctgcaaag gaccggccga aaaatggtgc tcggcaaaca
    18541 gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg
    18601 acaaccacag cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat
    18661 cgtgtggccc gacactattg ctacgatgtt tatccattct tagcgaagct gcggtagcga
    18721 gttgcgtttt taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg
    18781 tcagtttttt ccgcgccgcc agttccacca gccgttcagc atcgcgcaga ttttctgcca
    18841 gcggtttatc gacacagaca tgtacacccg cattgagtaa cgtactgacc acgtcaaagt
    18901 ggctggcggt gctggaatgc acaaaaaccg catcgcaact ggcggcaagg ctggataacg
    18961 aatcggcata aggaatgcgc cagctttcac aaattggcag ggctttcgcg cgcgtaggcg
    19021 accaggctcc ttgtaacatc cagtcagacg ctgccgccag caccggtaac cacgcttttt
    19081 gcgcaatgcc gcctaatccc acaacgccga tacgtaattt tttcacttta atctcccaga
    19141 tgggccagca acgaatcaag acgctgtttc agttctgcca cttcgatttc cagggcttcg
    19201 acgcgggcct gtaaatcacc atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc
    19261 tctacctcac cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc
    19321 agacgcacca caaaaggacc atcttcgcga tttgccagtt gttccagcgt cgactccaat
    19381 tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc
    19441 tgggcaccac gcaataacaa cgtggtgatt aacgccactt ccgctgcgct cagtttcaga
    19501 tcgccaaatt ctgaattaca aaaacgttgt tcatatttgg tgacccgatt accaaagcca
    19561 ctcaccgtgc gcagataatg gcgtttgacc agattatcca gttgctcctg cacttcagat
    19621 tcactcagat tcatgaccgg ttcacggttc gttttctgat tacaggccgt gactacacca
    19681 ttgactgaga gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc
    19741 acgcgagctt caagggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt
    19801 ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag
    19861 atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg
    19921 tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata
    19981 acgaatggct gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat
    20041 cgaataaccg agatagcagg catgaaaaga gccacgaaca acattggaaa aattggcaac
    20101 gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt
    20161 atgaaattcg ttaatcatcc ccagcctggc ctgccagcct gatggataac agtggctttc
    20221 gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgcaag
    20281 acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac
    20341 ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt
    20401 aatacgctgt aagtattact atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc
    20461 attttctgca ttattcacat atttgataaa aactctctat caaagcgacg ttttgataaa
    20521 aaaatattgt ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct
    20581 catcttttat ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa
    20641 atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct
    20701 gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct
    20761 cggtctacgc cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg
    20821 ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac
    20881 aatgggtatc gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg
    20941 cggcacgttg tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca
    21001 acgtggtcgt tttttctcgc tgttgatgat gcaggacagt gccggtgcgg tcattggcgc
    21061 attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt
    21121 tttgtttgtg ctgtgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac
    21181 cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac
    21241 ctatgtcctg acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc
    21301 aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga
    21361 agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg
    21421 tctggaacac cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg
    21481 catggtcagc ggcctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat
    21541 cattgccgag cctgcgcgtg aaaccttaag tgcttcgctg gcggacgcaa gagctcgcgg
    21601 cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg
    21661 tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat
    21721 gctgggaatt attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg
    21781 cgccgcgcgt cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca
    21841 tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca
    21901 ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt
    21961 acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt
    22021 tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta
    22081 tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga
    22141 gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc
    22201 ggcatttgat gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc
    22261 cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag
    22321 cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggagaact
    22381 ttcggaagat gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat
    22441 caactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa
    22501 tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt
    22561 gccgccgttt tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt
    22621 gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg
    22681 ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca
    22741 ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt
    22801 tacccggcgt ggctgacgcc catattgttc tgacaaactt gaccagccaa attggtcgcg
    22861 aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg
    22921 gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa
    22981 aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa
    23041 tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta
    23101 accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa
    23161 aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat
    23221 gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
    23281 ttgtgtccgg caaaaacatc ccttcagccg gagcatagag attaatgact gcaccatccc
    23341 aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
    23401 taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctattgta atgcccaatc
    23461 tggctccgcc cgtgaccact gttgaggctg ccgtggcgta tcgccagcgt attcttgatg
    23521 ccgtacctgc cgggcacaat ttcaccccat tgatgacctg ttatttaaca gattcgctgg
    23581 atcctaatga gctggagcgc ggatttaacg aaggcgtgtt caccgctgca aaactttatc
    23641 cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg
    23701 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg
    23761 cagatatcga catttttgat cgtgaagcgc actttataga aagcgtgatg gaacctctgc
    23821 gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg
    23881 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
    23941 ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc
    24001 tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag
    24061 tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct
    24121 gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
    24181 tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatggct
    24241 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
    24301 tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg
    24361 ttaaacaata aaaatcgtag cttcctgttg tcattaggtt attttacctg tataaataac
    24421 cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
    24481 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
    24541 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt
    24601 attggcgtaa caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa
    24661 agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg
    24721 ccccggcttt tttattttac ttacaaattt tgagtacaaa atttctacac ttcctcaaaa
    24781 taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc
    24841 ttaaaagcag aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt
    24901 ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa
    24961 cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt
    25021 agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca
    25081 gaccccaaat aagtccgagc aggaagggac tgaagttggt cagacgctct ggttaaccac
    25141 tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg
    25201 tgatttcccg gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg
    25261 cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg
    25321 cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg
    25381 ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac
    25441 cgacgcccat atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg
    25501 ccatgcttat ggtgaaggcg aaaagtatgt cccgttggtc ctccgcgcgc aaacgctgtg
    25561 ggatgaactt tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa
    25621 cctcggcccg gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca
    25681 actcaacgtt gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt
    25741 cccggacaac tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc
    25801 gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc
    25861 ggtcaccgca attcgtcatg acgatgatgg cgtaactatt gaaaccgttg acggtgagta
    25921 tcaggcgaaa aaagcgattg tctgcacggg aacatgggta aaagacctgc tcccggagct
    25981 gcctgtccag cctgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt
    26041 gaagaataaa ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt
    26101 tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc
    26161 agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt
    26221 cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga
    26281 taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct
    26341 cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc
    26401 cgattttgcg caagacaaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt
    26461 ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt
    26521 cggctgttgc actatctcat caataatatt cgcgagcatc tgatgcttta tctttttctc
    26581 tggggattgt tggcaattat ggatctgatc tatgtgtttt atttttagtt ttccgcactg
    26641 acataaactg acaaatattt aatctccctt tcatttagcg tgaaaaattg aatgaaatta
    26701 ttcctgcttc tacagcactc accagacatc tctgatgctg aacccactaa acttatgaag
    26761 attctttcat tattaattca aaattattga aataaaaata aatatcattc aatttgttat
    26821 ttttgtgttg caattcattc tccgacacgc cattttcttt ctctcgcaaa ttcgtattgg
    26881 atgcacttat gtcattcaca aatacccctg aacgctatgg tgttatatct gcggccttcc
    26941 attggctaag cgcaattatt gtctacggca tgtttacttt aggtctgtgg atggtcacgc
    27001 tcagttatta cgatggctgg tatcacaaag cacccgaact gcataaaagc atcggtattt
    27061 tgttaatgat ggggctggtt attcgcgttc tgtggcgtgt catatctcca ccgccgggtc
    27121 cgctgccgag ttattcgccc atgactcgtc ttgccgcaaa ggctggacat ctcgcattgt
    27181 acctattgct gttcgccatc ggcatcagcg gctatctgat ctcaactgcc gatggtaaac
    27241 cgatcagcgt ttttggctgg tttgacgtcc ccgcgaccct tgccgacgct ggcgcacagg
    27301 cagactttgc cggtgccctg catttttggc tcgcgtggag cgtcgtagta ctgtccgtta
    27361 tgcacggatt tatggccctg aagcatcatt tcatcgataa agacgacact ctgaagcgca
    27421 tgctgggaaa gtcgtcatct gactatggag tataaaaaat gaaaaaaagc ctgcttggtt
    27481 taaccttcgc gtccctgatg ttctctgccg gttcagcggt tgccgccgat tacaaaattg
    27541 acaaagaagg tcagcacgcc tttgttaatt tccgcatcca gcaccttggc tatagctggt
    27601 tatacggcac ctttaaagat ttcgacggta cttttacctt tgacgaaaaa aatccggctg
    27661 ccgataaagt gaatgtgaca attaacacca ccagcgtcga tactaatcac gccgaacgcg
    27721 ataaacatct tcgcagtgca gatttcctca ataccgcaaa atatccacag gcaacattca
    27781 cctccaccag cgtgaagaaa gacggtgacg aactggatat taccggcgat ctgacgctga
    27841 atggcgtaac caaacccgtc acgctggaag cgaaattaat tggtcagggc aacgacccat
    27901 ggggtggtaa acgtgcaggc ttcgaggccg aaggcaaaat taagctcaag gacttcaata
    27961 tcaagacaga tttaggtcca gcttctcagg aagtggatct gattatttca gtggaaggcg
    28021 tacagcagaa gtaagcgtga aatgttgccg gatggcacgc atccggcaat gatatttatt
    28081 ctgttggatc aggaatgccc agtgttgtat tcagacgtcc acgagactta ttaaagatct
    28141 tattgccatt ttcacgtcct gcccgacggc gtcgctgttc ttctggcggt aacgcgcttt
    28201 cttcgcagca aatctcacta caacaacctt tgtatttttc cgcgcatact ggacactgaa
    28261 taaacagcag gtggcagcca tcatttttac agttggtatg gctgtcgcac ggcgcgccgc
    28321 actggtggca atgcgcgata atctcatccg atatacgttc gcccatccgc tcgtcaaaaa
    28381 caaaattttt gccaataaaa cgcaccggca agccctgctc gcgcgcctta cgggcgtatt
    28441 caataattcc gccctcgata tgccagactt tgttgaatcc gttatgtttc atccaagcac
    28501 tggccttttc acaacgaatg ccgccggtgc agtacatgac gattttttta tctttatgtg
    28561 cctgcatcat ctcgactgct tttggcagct gctcacggaa ggtatctgcc ggaatttcga
    28621 gcgcgttttc aaagtgcccc acttcatact catagtggtt acg
//
LOCUS       DAJTSL010000037        28414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTSL010000037 DAJTSL010000000
VERSION     DAJTSL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.037"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(381..1481)
                     /gene="chaA"
                     /locus_tag="OO433_001457"
     CDS             complement(381..1481)
                     /gene="chaA"
                     /locus_tag="OO433_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6049984.1"
                     /translation="MSNAQEAVKTRHKEISLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1751..1981
                     /gene="chaB"
                     /locus_tag="OO433_001458"
     CDS             1751..1981
                     /gene="chaB"
                     /locus_tag="OO433_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6049985.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2139..2834
                     /gene="chaC"
                     /locus_tag="OO433_001459"
     CDS             2139..2834
                     /gene="chaC"
                     /locus_tag="OO433_001459"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6049986.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2878..3231)
                     /gene="ychN"
                     /locus_tag="OO433_001460"
     CDS             complement(2878..3231)
                     /gene="ychN"
                     /locus_tag="OO433_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6049987.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3415..4810
                     /gene="ychO"
                     /locus_tag="OO433_001461"
                     /pseudo
     CDS             3415..4810
                     /gene="ychO"
                     /locus_tag="OO433_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
     gene            complement(4811..5461)
                     /gene="narL"
                     /locus_tag="OO433_001462"
     CDS             complement(4811..5461)
                     /gene="narL"
                     /locus_tag="OO433_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6049988.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5454..7250)
                     /gene="narX"
                     /locus_tag="OO433_001463"
     CDS             complement(5454..7250)
                     /gene="narX"
                     /locus_tag="OO433_001463"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6049989.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRVRLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPASSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7589..8980
                     /gene="narK"
                     /locus_tag="OO433_001464"
     CDS             7589..8980
                     /gene="narK"
                     /locus_tag="OO433_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6049990.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9496..13218
                     /locus_tag="OO433_001465"
     CDS             9496..13218
                     /locus_tag="OO433_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6049991.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERAYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNQLMLTLGRGGPVVWLSEADAKELGIADNDWIEVYNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITGQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            13236..14774
                     /gene="narH"
                     /locus_tag="OO433_001466"
     CDS             13236..14774
                     /gene="narH"
                     /locus_tag="OO433_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6049992.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            14771..15481
                     /gene="narJ"
                     /locus_tag="OO433_001467"
     CDS             14771..15481
                     /gene="narJ"
                     /locus_tag="OO433_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6049993.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEVIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            15481..16158
                     /gene="narI"
                     /locus_tag="OO433_001468"
     CDS             15481..16158
                     /gene="narI"
                     /locus_tag="OO433_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6049994.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFAGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(16341..16472)
                     /locus_tag="OO433_001469"
     ncRNA           complement(16341..16472)
                     /ncRNA_class="other"
                     /locus_tag="OO433_001469"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(16520..16604)
                     /locus_tag="OO433_001470"
     tRNA            complement(16520..16604)
                     /locus_tag="OO433_001470"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16568..16570),aa:Tyr,seq:gta)
     gene            complement(16639..16723)
                     /locus_tag="OO433_001471"
     tRNA            complement(16639..16723)
                     /locus_tag="OO433_001471"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16687..16689),aa:Tyr,seq:gta)
     gene            complement(16883..17725)
                     /gene="purU"
                     /locus_tag="OO433_001472"
     CDS             complement(16883..17725)
                     /gene="purU"
                     /locus_tag="OO433_001472"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6049995.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(17775..18233)
                     /locus_tag="OO433_001473"
     CDS             complement(17775..18233)
                     /locus_tag="OO433_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6049996.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            18346..19251
                     /gene="rssA"
                     /locus_tag="OO433_001474"
     CDS             18346..19251
                     /gene="rssA"
                     /locus_tag="OO433_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6049997.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQCREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRVLGADIVIAVDLQHDAHLIQQDLLSFNVSEENSENGESLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            19343..20356
                     /gene="rssB"
                     /locus_tag="OO433_001475"
     CDS             19343..20356
                     /gene="rssB"
                     /locus_tag="OO433_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6049998.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDATVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            20558..21466
                     /gene="galU"
                     /locus_tag="OO433_001476"
     CDS             20558..21466
                     /gene="galU"
                     /locus_tag="OO433_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6049999.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEQVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(21610..22023)
                     /gene="hns"
                     /locus_tag="OO433_001477"
     CDS             complement(21610..22023)
                     /gene="hns"
                     /locus_tag="OO433_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6050000.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            22628..23245
                     /gene="tdk"
                     /locus_tag="OO433_001478"
     CDS             22628..23245
                     /gene="tdk"
                     /locus_tag="OO433_001478"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6050001.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(23527..24423)
                     /locus_tag="OO433_001479"
                     /pseudo
     CDS             complement(23527..24423)
                     /locus_tag="OO433_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(24547..27222)
                     /gene="adhE"
                     /locus_tag="OO433_001480"
                     /gene_synonym="adhC"
     CDS             complement(24547..27222)
                     /gene="adhE"
                     /locus_tag="OO433_001480"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6050002.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGILSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            27700..28347
                     /gene="ychE"
                     /locus_tag="OO433_001481"
     CDS             27700..28347
                     /gene="ychE"
                     /locus_tag="OO433_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6050003.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         6967 a         6832 c         7405 g         7210 t
ORIGIN      
        1 cattttgcgg ctattcttga agtgtctggt ttcaagatta gcccccgttt tgttgtcagg
       61 ttttacctct caacgtgcgg gggttttctc tttccagcaa ccaatgccac cagggataaa
      121 gcccccgcaa cattgcgcct caccggataa ttccggcttg gtgtggatac tacgtctcaa
      181 ttcatcttca cttcatccct gaaatgtttg caataaagag tacattccgg cttttcaaca
      241 gctgttgcag tggtttcgtg caggttgtgg tacaggctcg caattctgat taacgagctg
      301 tccaggtagt atgaagaaat acaataagca ggaatttatc ttatctcccc cgatttccgt
      361 catcggagga gatataactg tcaggcaaat atcgtcatca aataggcggc aaacagtgcc
      421 agatgcgctg cgccattgag cacgttagta cgtccggtgg agaaggagat atggcacagc
      481 actaaagagg ccaccatcac caccatttct ggcgcaccaa gtgcaaactg taattcgtta
      541 cccgtcataa aggcaattag cgtgacgaca ggtacggtaa gtgaaatggt tgctaacact
      601 gaaccaaaga acagattcat cgcgcgctgg acctggttgt tcaacactgc ttttaacgca
      661 cctaaacctt ccggcgacag aatcaacaga gccaccagga agccagtaaa ggcgacaggg
      721 gcattcatgc tgtcgagcaa tgtctccagc gggctggcat tcattttggt gactgcaata
      781 acggcaatca gatggataat caaccagata gcatgccaca cgctgctatg ggcagacggt
      841 ttaccgtgat gcgggtcgtc gtcatcactg tcatcttcgt gctcgtagac aaacaaactt
      901 tgatgcgttt tggtctggat cagcaaaaat acgccataca ttgccgcaga aattaatgct
      961 accagtaacg cctgaccggt tgaaaaattc gccgcaggca gagccattgg aaataccagt
     1021 acgattatcg ccagggggaa cagcgcaatt aaatactgct tgataccaaa cagattcata
     1081 tattgggtgg caaacttacg accgcccaac aataatgaaa agccaaccag cccaccggta
     1141 acaatcataa tgattgaata gagcgtatca cgcattagcg ttggcgcggc gtcgccggtt
     1201 gccattaaag ctgaaatcaa actgacttca agaataacca ctgaaaggct aagaataagc
     1261 gaaccgtaag gttctcccag gcgatgggct aatacgtccg catgacggac aacactaaag
     1321 gcgctactta aaataccaat aagcgcaaga agattgatgg caatgaccac tggtagtgtc
     1381 tggctgcttc cccacaggaa cagcactacc agcgccagaa ccgggaaaat aagcgaaatc
     1441 tccttgtggc gggtttttac cgcctcttga gcatttgaca ttatggttat ccctttgcag
     1501 atgaatttat cgaaaatgta aaaaataggt aggaaaaata acagaaagtg tctggatatc
     1561 ggtaacattt tacgaatttt taccccactg tcgatttttt actcattggg acataaaaat
     1621 aagtattacg tttagacaat gtttgtttta gcgtttctta agaacagtct gacatgaaaa
     1681 ttcttatgtt ttggcaagag tagatattgt tgaccacact taatgttcaa ctttgtaaaa
     1741 ggagtcaacg atgccgtata aaacgaaaag cgatctgccg gaaagcgtaa agcacgttct
     1801 accgtctcat gcccaggata tctataaaga agcgttcaac agcgcatggg atcaatataa
     1861 agataaagac gatcggcgtg atgacgccag tcgcgaagaa accgcgcata aagtggcctg
     1921 ggctgctgtg aagcatgaat atgccaaagg ggatgatgat aaatggcata aaaaatcgta
     1981 aaaccggtag cttagttaaa gctattcgtg cggtgttgcc ttgcaagtgg tccgtggatt
     2041 gcatattgtc ccgttagtgg tttcaaaatg agcagtaaaa atgtccggaa gacaccaaaa
     2101 agttgtcgca gggaagtatg cagtggcgga ggtgtaaggt gataacgcgt gatttcttga
     2161 tgaatgccga ttgtaaaacg gcatttggtg ccattgaaga atcactctta tggtcagcag
     2221 agcaacgagc ggcttcgctg gcggcgacgc tggcttgtcg acctgatgag ggaccggtgt
     2281 ggatcttcgg ttatggatcg ttgatgtgga atccggcact ggagtttacc gagtcgtgca
     2341 ccggtacact ggttggatgg catcgcgcgt tttgcctgcg cctgaccgcc gggcgcggga
     2401 ctgcgcacca gccgggacgg atgcttgcac tgaaagaggg cggacgcact acaggcgtcg
     2461 cctatcgact gccagaagag acgctggagc aggaactaac cctgttgtgg aagcgcgaga
     2521 tgattaccgg ctgttatctg ccaacctggt gtcagcttga tcttgatgat ggatgcacag
     2581 taaacgccat tgtgtttatt atggacccgc gacatccaga atatgaatct gacactcgcg
     2641 ctcaggtcat cgcgccgttg attgcggcgg cgagcggtcc gctgggaacc aacgcacaat
     2701 acctgttttc actggaacag gagcttatca aactgggaat gcaggacgat gggctgaatg
     2761 atttgctggt atcggtaaaa aaactgctgg cggagaatta tccggatggt gtgttacggc
     2821 cgggattcgc ctgagtaaac ttcccgcata gtggggcgtc agacgccccc tcaaacatta
     2881 aaatgtgagc actttatcgg ctgacagcgt ccattgcgcc agttccacaa gagtaccgat
     2941 ttccacccca tcaatcagag gaagtgtact aatcccgcgc ccgtcggtac aggttttgca
     3001 caatttcacc ggtacattct gagcggtaag gatctccagc atttgctgaa tgttgtagcc
     3061 ttcccctggt ttttgcccgc gcaacccggc tgtgaccgca tcagacatga ggaacagacg
     3121 cagatccaga ttgctctcct gctctcgtaa cgcaatggcc agccgcaagc tgttaaacaa
     3181 ggattcgctc ccgtaaggtg cgccattggc aacgatcacg attttttgca ttatttactc
     3241 ctgtattcag ggaattagac actcatcttc tatcttactg cttctgcagc gtctgaccaa
     3301 tcggtcacat ttttaaggat tttcctgaaa gcgcgagaaa atacgacaaa agttgccagt
     3361 aatcgttatt ctttaaggct atggttttca ttattaccgg aagttaccga cgttttgagc
     3421 cgtttcgttc ctcgcattat cccgttttat ttactcttgc ttgtagcagg cggtacagct
     3481 aacgcacaat ctaccttcga gcaaaaagcg gcaaatccct ttgataataa caatgatggt
     3541 ctgccggatt taggcatggc tcccgaaaat catgatgggg aaaaacactt tgctgaaatt
     3601 gtgaaagatt tcggcgaaac cagtatgaat gataacgggc tggatactgg cgagcaggca
     3661 aaagctttcg cattgggaaa agtccgcgac gcgcttagtc aacaggttaa tcagcacgta
     3721 gagtcctggc tatcaccgtg gggaaatgcc agtgttgacg tcaaagtgga taacgaagga
     3781 catttcaccg gcagtcgtgg aagctggttt gtgccgttac aagataatga tcgttatttc
     3841 acctggagcc agcttggtct tactcagcag gataatgggt tggtgagcaa tgtgggcgtt
     3901 gggcaacgct gggcgcgcgg caactggctg gtgggttata acacttttta tgacaacttg
     3961 ctggacgaaa atcttcagcg agcgggcttt ggtgccgaag cgtggggcga atatttgcga
     4021 ttattggcaa acttttatca gccgtttgct gcatggcatg aacagacagc cacgcaggaa
     4081 caacggatgg cgcgcgggta cgacctgaca gctcggatgc gcatgccgtt ctatcaacac
     4141 ctcaatacca gtgtcagcct agaaacagta ttttggtgat cgtgttgatt tgtttaactc
     4201 tggtacgggt tatcacaatc ccgtcgcgtt gagtctggga ttaaattaca cccctgtgcc
     4261 attagtcact gtgacggccc agcataaaca gggtgaaagt ggcgagaatc aaaataacct
     4321 cgggctgaat cttaattacc gctttggtgt accgctcaaa aaacaacttt ctgcgggcga
     4381 ggttgccgaa agtcagtcgt tacgtggtag tcgctatgac aatccgcagc gaaataatct
     4441 accgactctt gagtaccgac agcgaaaaac gttaacggtg tttctggcga caccgccgtg
     4501 ggatctaaaa cctggcgaaa cagtgccgct gaaattacaa atccgcagtc gttacggtat
     4561 tcggcaactg atttggcagg gcgatacgca gatattaagt ttgacgccgg gcgcacaagc
     4621 caacagcgcg gagggctgga cgctgatcat gcctgactgg cagaacgggg aaggggcaag
     4681 caatcactgg cgattgtcgg tggtggtgga atataaccag gggcagcgtg tctcctccaa
     4741 tgagatcacg ctaacgcttg tcgaaccgtt cgatgcattg tcaaacgacg aactgcgctg
     4801 ggaaccgtaa tcagaaaatg cgctcctgat gcacccatac cgctgcttcc acgcgagact
     4861 tgagcttcat tttcttcagc atgtgcttga cgtgcacttt tactgtgctt tcggtgatat
     4921 ccaggcggcg ggcaatcatc ttgttcggca aaccctgggc aatcagcttg agaatatcgc
     4981 gctcgcgtgg ggttaactgg ttaacatcgc gctcagtagt ggcacggtta gcgcgcaagc
     5041 tggcggccag aacaggcgtt aatgcttcgc ttaataccat ttcgccagca gctgcctgat
     5101 gcaatgcttt cagcagatct tccggttcca tatcttttaa cagataacca tccgcgccgc
     5161 gtttcagtgc ggtgaccaca tcttcttcat ggttagagac gctgaatacc acaatgcgcc
     5221 ctgagaggga cttttcgcgc agtttatcca gcgtttccag accgttcatg ccgggcatat
     5281 tgagatctaa caggatcaga tcgggatcaa gagactccgc cagttcaata ccctgttcgc
     5341 cattactcgc ttcgccaacc acggtgatat ctggtgccat actgataagc tgttttacgc
     5401 cagttcgcag catcgggtga tcgtcgatca gcaggatagt agccggttcc tgattactca
     5461 tgggtatctc cttggacgtc tgtgaaagtt ttttcgggaa taaaggtgac caccacttcg
     5521 gtgccacctg attcacgacg gcggacgcgg caatcgcctc gtaaactttg cgcacggtcg
     5581 cgcattatta tcatgccgta gtgattgctg cggatggcat tttcaggcac gccgcagccg
     5641 ttatcttgga cggtcagttt gacttgatta tcgttttgcg ccaccgtcac cacgacctca
     5701 ctcgcttgcg aatgtttgag ggcgttactt aatgcctcac gggcaatttg caacaagtgg
     5761 attgcctgat gtgaaggcac cagacgaggc ggcaattgat aatccagctt caccgggaag
     5821 ccaaatttgg cgctgtactc ttcgcaactc gcctccagcg ccggacgtaa tccaggctcg
     5881 gtgagctgca agcggaatgt ggtgagcaat tcacgcaact gcgcccagga tgcattcagt
     5941 tcgttacgga tctgacttaa cagttcgcgg ctgcttgctg gcagcgcatc gccctgcatc
     6001 tgtaaacaac tcacctgcat cttcatgcaa gagagagatt gggcaataga atcatgcagt
     6061 tcgcgcgcaa tggtggcacg ctcttccatc acgatcaact gttgctgacg ttcctgatgg
     6121 cgatccagcg ccagcgtggc ggtgagttgt tcaaccaggg tatccaccag ttgttgttga
     6181 tcatggctaa gatgacgccc ctgcggcagg gtcgccagca aaataccgta ctgcgtatga
     6241 gagtcagcca gccgccactt cagggttgtg ccgcgatcac caacgggtaa tacgccgcgc
     6301 gggcagagct ggcagccttt atcatcacaa gtcatatctg gctggcaggt aaactcctga
     6361 tgattctctt catcatcagt gtcatacacc cgcagttcga tatcacgtag cagggttaaa
     6421 ttctgtaagc cgttgagtac aggtgacagg cgttcacaca gcggggcgcg ggaatgcaaa
     6481 cggcggttag cctgccataa aaaagagagg atctgatttt tatgctccag cccggcggtt
     6541 ttctcctgaa cccgctgctc aagtacggca taactttcgg ccagttctgc agacatattg
     6601 ttcaatgcag ttccaagcat cgccatttcg ttgcgcccgc tgatgttcgc gcgttgggta
     6661 aaatcgcgat gactgacggc actcgccatt gccagcagtt gccgccacgg ttgtagcagt
     6721 cgcacccgca accagataat agtgaacacc agtaaaagtg ccataaatac cgccattacc
     6781 cgatggacca gtaccactgt ctcaatgcgc atttccgtgg tgcggtcaaa accagatacc
     6841 agctgatcaa gcccggcaac aaactggctg acatccgctg acaccgtttc gcggttttgt
     6901 gcacgcatca gtgcagggat cagttcatta cgccagtaat cttgtaaacc ctgtaattgc
     6961 gccagttgtt cgtctcgttc tgctgctcga gttaactcgg cgctaaatgc cgtttgttcc
     7021 atctctttaa ttaagggctt gtctttctca cttaatggca ctgccgccaa cagacggtaa
     7081 ctttgcatgc gcagcgatcc cgctttgttg atcgcatggg cgctgccctg aacgccttgc
     7141 accagccagc cagaaaccgc catccctgcc agtccaatag cagtagaaag caacacaata
     7201 agcgcaacct gattaaccag ggtgagcgga gagagacaac gtttaagcat gtaaacctct
     7261 tccttcaggc tttaaatgag caataacctt aatgaatgtg acgatacatt ctggaatggc
     7321 agtattctcg gctattggct gaagtatacc catacccgga aagagttact ccttatttgc
     7381 cgtgtggtta gtcgctttac atcggtaagg gtagggattt tacagcaccg tgaaaaatct
     7441 cataattttt atgaaatcac tgtactcact atgggtaatg ataaatatca atgatagata
     7501 aagttatctt atcgtttgat ttacatcaaa ttgcctttag ctacagacac taaggtggca
     7561 gacatcgaaa cgagtatcag aggtgtctat gagtcactca tccgcccccg aaagggctac
     7621 tggagctgtc attacagatt ggcgaccgga agatcctgcg ttctggcaac aacgcggtca
     7681 acgtattgcc agccgtaacc tgtggatttc cgttccctgt ctgctgctgg cgttttgcgt
     7741 atggatgttg ttcagcgctg ttgcggtgaa cttaccgaaa gtcggtttta attttacgac
     7801 cgatcagcta tttatgttga ctgcgctgcc ttcggtttct ggcgcgttat tacgtgttcc
     7861 atactccttt atggttccta tcttcggtgg tcgtcgctgg acggcgttca gcaccggtat
     7921 tctgattatt ccttgcgtct ggctgggttt tgccgtgcag gatacctcca cgccttatag
     7981 cgtcttcatc atcatctctc tgctatgcgg ctttgctggc gcgaacttcg catccagtat
     8041 ggcaaacatc agcttcttct ttccgaaaca gaagcagggt ggcgcgctgg gtctgaatgg
     8101 tggtctggga aacatgggcg tcagcgtcat gcagttggtt gctccgctgg tggtatcact
     8161 gtcgattttc gcagtatttg gtagccaggg cgtcaaacag ccggatggga ctgagctgta
     8221 tctggcgaat gcgtcctgga tatgggtgcc gttccttgcc atcttcacca ttgcggcgtg
     8281 gtttggcatg aacgatcttg ctacctcgaa agcctccatc aaggagcagt tgccggtact
     8341 caaacggggt catctgtgga ttatgagcct gctgtatctg gcaaccttcg gctccttcat
     8401 cggcttctcc gcgggctttg caatgctgtc aaaaacgcag ttcccggatg ttcagattct
     8461 gcaatacgct ttcttcgggc cgtttattgg tgcgctggcg cgttctgcag gtggtgcatt
     8521 atctgaccgt ctgggcggaa ctcgtgtcac gctggtgaac tttattctga tggcgatttt
     8581 cagcggcctg ctgttcctga ccttaccgac tgacgggcag ggcggaagct tcatggcgtt
     8641 cttcgcggtc ttcctggcgc tgttcctgac agctgggctg ggtagtggtt ccactttcca
     8701 gatgatttca gtgatcttcc gtaaactgac aatggatcgc gtgaaagcag aagggggttc
     8761 tgacgaacgt gcgatgcgtg aagcggcaac cgacacggcg gcggcgctgg gtttcatctc
     8821 tgcgattggc gcgattggtg gcttctttat cccgaaagcg tttggtagct cgctggcatt
     8881 aacgggttcg ccagtcggcg caatgaaggt atttttgatt ttctatatcg cctgcgtagt
     8941 gattacctgg gcggtatatg gtcggcattc taaaaaataa accgttactc gtcatactcc
     9001 gggttacatg tgctgcggct gcgttcattc accccagtca cttactttag taagctcctg
     9061 ggattcattc acttgccgcc ttcctgtaaa ccgaattata tagagtaaaa tatttgatta
     9121 tcctttgcgc ggcatgatgt cgcgcttttt ttaagcgtta tttagttaca acatactaat
     9181 gttatatggt ttatttcgcc ggatttcatt aagagccatt aatatgttac ccatggggaa
     9241 tactccttaa tacccatctg cataaaaatc ttaatagttt aaataactac aggtataaaa
     9301 cgtcttaatt tacagtctgt tatgtggtgg ctgttgatta tcctaaaggg gtatcttagg
     9361 aatttacttt atttttcatc cccatcactc ttgatcgtta tcaattccca cgctgtttca
     9421 gagcgttacc ttgcccttaa acattagcaa tgtcgattta tcagagggcc gacaggctcc
     9481 cacaggagaa aaccgatgag taaattcctg gaccggtttc gctacttcaa acagaagggt
     9541 gaaacctttg ccgatgggca tggccagctt ctcaatacca accgtgactg ggaggatgga
     9601 tatcgccagc gttggcagca tgacaaaatc gtccgctcta cccacggggt aaactgcacc
     9661 ggctcctgca gctggaaaat ctacgtcaaa aacggtctgg tcacctggga aacccagcag
     9721 actgactatc cgcgtacccg tccggatctg ccaaaccatg aacctcgcgg ctgcccgcgc
     9781 ggtgccagct actcctggta tctttacagt gccaaccgcc tgaaataccc gatgatgcgc
     9841 aaacgcctga tgaaaatgtg gcgtgaagcg aaggcgctgc atagcgatcc ggttgaggca
     9901 tgggcttcta tcattgaaga cgccgataaa gcgaaaagct ttaagcaggc gcgtggacgc
     9961 ggtggatttg ttcgttcttc ctggcaggag gtgaacgaac tgatcgccgc atctaacgtt
    10021 tacaccatca aaaactacgg cccggaccgt gttgctggtt tctcgccaat tccggcaatg
    10081 tcgatggttt cttacgcatc gggtgcacgc tatctctcgc tgattggcgg tacttgctta
    10141 agcttctacg actggtactg cgacctgcct cctgcgtctc cgcaaacctg gggcgagcaa
    10201 actgacgtac cggaatctgc tgactggtat aactccagct acatcatcgc ctggggttca
    10261 aacgtgccgc agacgcgtac cccggatgct cacttcttta ctgaagtgcg ttacaaaggc
    10321 accaaaactg ttgccgtcac accagactac gctgaaatcg ccaaactgtg cgatctgtgg
    10381 ctggcaccga aacagggcac cgatgcggca atggcgctgg cgatgggcca cgtaatgctg
    10441 cgtgaattcc acctcgacaa cccaagccag tatttcaccg actatgtgcg tcgctacacc
    10501 gacatgccga tgctggtgat gctggaagaa cgcgacggtt actacgctgc aggtcgtatg
    10561 ctgcgcgctg ctgatctggt tgatgcgctg ggccaggaaa acaatccgga atggaaaact
    10621 gtcgccttta ataccaatgg cgaaatggtt gcgccgaacg gttctattgg cttccgctgg
    10681 ggcgagaagg gcaaatggaa tcttgagcag cgcgacggca aaactggcga agaaaccgag
    10741 ctgcaactga gcctgctggg tagccaagat gagatcgctg aggtaggctt cccgtacttt
    10801 ggtggcgacg gcacggaaca cttcaacaaa gtggaactgg aaaacgtgct gctgcacaaa
    10861 ctgccggtga aacgcctgca actggctgat ggcagcaccg ccctggtgac caccgtttat
    10921 gatctgacgc tggcaaacta cggtctggaa cgtggcctga acgacgttaa ctgtgcaacc
    10981 agctatgacg atgtgaaagc ttataccccg gcctgggccg agcagattac cggcgtttct
    11041 cgcagccaga ttattcgcat cgcccgtgaa tttgccgata acgctgataa aacgcacggt
    11101 cgttcgatga ttatcgtcgg tgcggggctg aaccactggt atcacctcga tatgaactat
    11161 cgtggtctga tcaacatgct gattttctgc ggctgtgtcg gtcagagcgg gggcggctgg
    11221 gcgcactatg taggtcagga aaaactgcgt ccgcaaaccg gctggcagcc gctggcgttt
    11281 gcccttgact ggcagcgtcc ggcgcgtcac atgaacagca cttcttattt ctataaccac
    11341 tccagccagt ggcgttatga aaccgtcacg gcggaagagt tgctgtcacc gatggcggac
    11401 aaatcccgct ataccggaca cttgatcgac tttaacgtcc gtgcggaacg catgggctgg
    11461 ctgccgtctg caccgcagtt aggcactaac ccgctgacta tcgctggcga agcggaaaaa
    11521 gccgggatga atccggtgga ctatacggtg aaatccctga aagagggttc catccgtttt
    11581 gcggcagaac aaccagaaaa cggtaaaaac cacccgcgca acctgttcat ctggcgttct
    11641 aacctgctcg gttcttccgg taaaggtcat gagtttatgc tcaagtacct gctggggacg
    11701 gagcacggta tccagggtaa agatctgggg caacagggcg gcgtgaagcc ggaagaagtg
    11761 gactggcagg acaatggtct ggaaggcaag ctggatctgg tggttacgct ggacttccgt
    11821 ctgtcgagca cctgtctcta ttccgacatc attttgccga cggcgacctg gtacgaaaaa
    11881 gacgacatga atacttcgga tatgcatccg tttattcacc cgctgtctgc ggcggtcgat
    11941 ccggcctggg aagcgaaaag cgactgggaa atctacaaag ccatcgcgaa gaaattctcc
    12001 gaagtgtgcg tcggccatct gggtaaagaa accgacatcg tcacgctgcc tatccagcat
    12061 gactctgccg ctgaactggc gcagccgctg gatgtgaaag actggaaaaa aggcgagtgc
    12121 gacctgatcc caggtaaaac cgcgccacac attatggtcg tagagcgcgc ttatccggcg
    12181 acttacgaac gctttacctc tatcggcccg ctgatggaga aaatcggtaa tggcggtaaa
    12241 gggattgcct ggaacaccca gagcgagatg gatctgctgc gtaagctcaa ctacaccaaa
    12301 gcggaaggtc cggcgaaagg ccagccgatg ctgaacaccg caattgatgc ggcagagatg
    12361 atcctgacac tggcaccgga aaccaacggt caggtagccg tgaaagcctg ggctgccctg
    12421 agcgaattta ccggtcgtga ccatacgcat ctggcgctga ataaagaaga cgagaagatc
    12481 cgcttccgcg atattcaggc acagccgcgc aaaattatct ccagcccgac ctggtctggt
    12541 ctggaagatg aacacgtttc ttacaacgcc ggttacacca acgttcacga gctgatccca
    12601 tggcgtacgc tctctggtcg tcagcaactg tatcaggatc accagtggat gcgtgatttc
    12661 ggtgaaagcc tgctggttta tcgtccgccg atcgacaccc gttcggtgaa agaagtgata
    12721 ggccagaaat ccaacggcaa cccggaaaaa gcgctcaact tcctgacgcc gcaccagaag
    12781 tgggggatcc actccaccta cagcgacaac cagttgatgc tgaccttagg tcgcggtggc
    12841 ccggtggtct ggctgagcga agccgatgcc aaagagctgg gcattgccga taacgactgg
    12901 attgaagttt acaacagcaa cggtgcgctg actgcccgtg cggttgtcag ccagcgtgtt
    12961 ccggcaggga tgaccatgat gtaccacgcg caggaacgta tcgttaacct gccgggttcg
    13021 gaaattactg gccagcgtgg ggggatccac aactcggtca cccgtatcac gccgaaaccg
    13081 acgcatatga ttggtggcta tgcccatctg gcatacggct ttaactacta cggcaccgta
    13141 ggttctaacc gcgatgagtt tgttgtagtg cgtaagatga agaacattga ctggttagat
    13201 ggcgaaggca atgactaggt acaggagagc gtaaaatgaa aattcgttca caagtcggca
    13261 tggtgctgaa tctcgataag tgcatcggct gccacacctg ttcagttacc tgtaaaaacg
    13321 tctggaccag ccgtgaaggc gtggagtacg cgtggttcaa caacgtggaa accaagccgg
    13381 gtcagggctt cccgactgac tgggaaaacc aggaaaaata caaaggcggc tggatccgta
    13441 aaatcaacgg caaactgcag ccgcgcatgg gtaaccgtgc catgttgctg ggtaaaatct
    13501 tcgctaaccc gcatctgccg gggatcgacg attattacga gccgttcgat tttgactatc
    13561 agaacctgca taccgcgccg gaaggcagca aatcgcagcc gattgcccgt ccccgttcgc
    13621 tgattaccgg ggaacggatg gcgaaaatcg aaaaagggcc gaactgggaa gatgacctgg
    13681 gtggtgagtt tgacaaactg gcgaaagaca agaacttcga caacatccag aaggcgatgt
    13741 atagccagtt cgaaaacacc tttatgatgt atttgccgcg cctgtgcgaa cactgcctga
    13801 acccggcatg tgtggcgacc tgcccgagcg gtgcgattta caagcgtgaa gaagatggca
    13861 tcgtcctgat cgaccaggat aaatgccgtg gctggcgtat gtgcatcacc ggatgcccgt
    13921 acaaaaaaat ctacttcaac tggaagagcg gtaagtctga gaagtgcatc ttctgctatc
    13981 cgcgtattga agcgggtcag ccgaccgtgt gctcagaaac ctgtgtcggt cgtatccgtt
    14041 atcttggcgt gctgctgtac gatgccgacg cgattgaacg tgcagccagc accgagaacg
    14101 agaaagatct ttaccagcgt cagctggagg tgttcctcga tccgaacgat ccgaaagtca
    14161 tcgagcaggc gattaaagac ggtattccgc tgagcgttat tgaagccgca cagcagtcgc
    14221 cggtttataa aatggcaatg gaatggaaac tggcgctgcc gctgcatcca gaatatcgca
    14281 cactgccgat ggtctggtac gtgccgcctc tgtctccgat tcagtctgca gcagacgcgg
    14341 gtgagctggg tagcaacggc attctgccag acgtcgaaag cttgcgtatt ccggtacagt
    14401 atctggcgaa tctgctgacc gccggtgata ccaaaccggt actgcgcgca ctgaaacgta
    14461 tgctggcgat gcgtcattac aaacgtgctg aaaccgttga cggtaaagtt gatacccgtg
    14521 cgctggaaga ggtcggtctg accgaagccc aggcacagga gatgtaccgt tatctggcta
    14581 ttgctaacta cgaagatcgc tttgtggtgc cgagtagtca tcgtgaactg gcacgggaag
    14641 ccttcccgga gaaaaatggc tgcggcttta cctttggtga tggctgccac ggctcagata
    14701 ccaaattcaa tctgttcaac agccgtcgta tcgatgccat cgatgtgacc agcaaaacgg
    14761 agccgcatcc atgatcgaac tcgtgattgt atcgcgtctc cttgaatatc cggatgctgc
    14821 cttatggcag catcaacaag agatgtttga ggtgattgct gcgtcgaaaa atctgtcgaa
    14881 agaagatgcc catgcgctgg gcattttcct gcgcgattta acggcgatgg atccgctcga
    14941 tgcccaggcc cagtacagcg aactgttcga ccgtggccgc gccacgtcac tgttgctatt
    15001 tgaacatgtt cacggtgaat cccgtgaccg cggccaggcg atggtggacc tgctggcgca
    15061 gtacgagcag cacggcttgc agttaaacag ccgcgaatta ccggaccatc tgccgctgta
    15121 tctggagtac ctgtcgcagc tgccgcaaag cgaagccgtg gaaggtttga aagatatcgc
    15181 gccgattctg gcattgctga gcgcgcgtct gcaacagcgt gaaagccgtt atgccgtgat
    15241 gtttgatctg ctgctgaaat tggccaatac cgctatcgac agcgacaaag tggcggaaaa
    15301 aattgccgac gaagcgcgcg atgatacgcc gcaggcgctg gatgctgtct gggaagaaga
    15361 gcaggttaaa ttctttgctg acaaaggctg cggtgattca gcaatcactg ctcatcagcg
    15421 tcgctttgcc ggtgccgtcg cgccgcaata tctgaatatc accaccggag gacagcacta
    15481 atgcaattcc tgaatatgtt cttctttgat atctacccgt acatagccgg ggcggtcttc
    15541 ctgattggta gctggctgcg ttatgactac gggcagtaca cctggcgcgc ggcgtccagc
    15601 cagatgctgg atcgcaaagg gatgaacctg gcgtcgaacc tgttccatat cggtattctg
    15661 gggatttttg ctggtcactt cttcggtatg ctgacgccgc actggatgta tgaagcctgg
    15721 ctgccgattg aagtgaaaca gaaaatggcg atgtttgctg gtggtgccag cggcgtgctg
    15781 tgtctgattg gcggcgtgct gttgctgaag cgtcgtctgt tcagcccacg cgtgcgtgca
    15841 accactaccg gagcggatat cctgatcctg tcgctgctcg ttatccagtg cgcgctgggc
    15901 ctgttgacca ttccgttctc cgctcagcat atggacggta gcgagatgat gaaactggtt
    15961 ggctgggcgc agtcggtggt gaccttccac ggtggcgctt ctcaacacct cgatggtgtg
    16021 gcgtttatct tccgtctgca cctggtgctg gggatgacgt tattcctgct gttcccgttc
    16081 tcgcgtctgg tacacatctg gagcgtaccg gtggagtatc tgacacgtaa gtaccagctg
    16141 gtgcgcgctc gtcactaaac gaattttagt tcacatagac cctgcttcgg cggggttttt
    16201 tatgggcacg gtgcggggtg agttgtcgga tgcgcttcgc ttatccgacc tacaggggag
    16261 gatattgtag gcccggtaaa cgtggtgcca tcgggcaaaa ttagctcgga gtaacaggtt
    16321 ttgattattc aaggaggcga tggtggtggg ggaaggatta ctcagcgctg cgcgcttcgc
    16381 ccttcgggtc gttgcctgcg gcaacgctct ctcgctggcg ctcaagtcga accttggtcg
    16441 aagcttctca tccttccccg cttgggcaga atatttaatt gcggattcgt ttgagagttt
    16501 acggactttt gaaaagtgat ggtggtgggg gaaggattcg aaccttcgaa gtcgatgacg
    16561 gcagatttac agtctgctcc ctttggccgc tcgggaaccc caccaggggt aattcaaatt
    16621 ttgaggtaat gcttgagatg gtggtggggg aaggattcga accttcgaag tcgatgacgg
    16681 cagatttaca gtctgctccc tttggccgct cgggaacccc accacggggt aatgcttttt
    16741 actggcctgc tcccttatcg ggaagcgggg cgcatcatat caaatgacgc gccgctgtaa
    16801 agtgttacgt tgagaaaaat gaactggttg cgtaattttc atccgtaacg gattaaaggt
    16861 aaccagttat ttttgctggc gattaaagaa taatcgtccg attaccgtaa acaaagacgc
    16921 gctgtgccag tactttgtat agcgcacgac ttaagacgtt tttctcgacg tcacgacctg
    16981 cgcgcatcat atcttcagct gtgtaggtat gatcgacatg aataacgtcc tgcatgatga
    17041 ttgggccttc gtccagattg tcattcacat agtgagcggt tgcgccaata atcttcacac
    17101 cacgttcata ggcctggtga taaggacgtg cgccgataaa cgcaggcagg aatgaatggt
    17161 gaatattgat gattttattc gggaagcgtg ccacaaattc cggcgttaat acccgcatat
    17221 acttcgccag caccacatag tcaggtttat aagcatcaat ggcgtctgcc atcttttgat
    17281 cgtgctcgtt gcggcttaac ccttcatggt ttaccagctc aaacggaata tcaaaacgct
    17341 caaccagaga acgtaaagta tcgtggttac caataactgc cgcgatttcg acatccaggc
    17401 cgccgtaatt ggctttcatc aacaaatcgc caaggcaatg cgcttcttta gtgaccagaa
    17461 tcactatccg gcgacgaccg gcaggattca gctcacgcac ggagccttct ggcaatgcgc
    17521 tatcgagatc cgccagcagg gtggaatcat taaaaatccc ttccagttcc gtgcgcataa
    17581 aaaagcgccc ggtacggtga tcaacaaatt cattgttctg tacgatattt aactcgtgct
    17641 tgtagcaaat attggtaata cgtgcgatca gacctttttg gtccggacaa atagtacgca
    17701 gaactttacg ttggagtgaa tgcattgctg gaaaaacctt gttgagagtg tttgctaaac
    17761 cgtaccgtca accattattg gccgcagcac tttttaaatt ttttacctga accacaaggg
    17821 cagggatcgt tgcgaccaaa ctgcggacgt gtaccgtcaa tatagtacca ttgaccgttt
    17881 tctttcaaaa atcgcgaacg ttcaattatt gcacctgttt tgccaccttc ggtaaatcga
    17941 gcaacaaaac tgacaaatcc gatattgtcg gcgtcctgcc aacaatgttc aaatacggtc
    18001 aatcccaacc attcagtatg cgcaaatccg gccatcaatt cggcacgtaa cgccgctgct
    18061 ccacaagagg gatgccaggt ctttattaaa taatctgcgt cttgcatcac aaaagcgcag
    18121 taacgcgaac gcatgagatg ttctggatca ggtgcaacct tttcaccaga cacataaggg
    18181 tggcaacata ggctatactc gacagcacta ccacagggac aaagctgaga cacaaataat
    18241 ctccctggaa acaataacgg cgtattaacc gcctgagtag cactatgtta accgagcagt
    18301 agcgatgtgg ctacgattgc attccagggg aatcttgcgg gaataatgag aaagataaaa
    18361 atagggctgg cgctgggatc tggcgcggcg agaggttggt cgcatattgg cgttattaat
    18421 gcgctaaaaa aagtgggtat tgaaattgat atcgttgcag gatgttcaat tggttcgctg
    18481 gtgggcgctg cctatgcatg cgatcgatta tctgcgctgg aagattgggt gacctctttc
    18541 agttattggg atgttttacg cctgatggat ctctcctggc agcgcggtgg gttactgcgc
    18601 ggcgagcgtg tcttcaatca atgccgcgaa ataatgccgg aaacagagat cgaaaattgt
    18661 tcccgtcgct ttgcggctgt tgccaccaat ttaagtacgg gacgtgaatt atggtttact
    18721 gaaggcgatc tccatcttgc tattcgcgca tcatgcagta ttccaggact catggcacct
    18781 gttgcacata acggctactg gctggttgat ggagcagtag ttaacccaat tcctatttcc
    18841 ctcacgcgtg tattgggggc tgatattgtg atagcggtcg acctgcagca cgatgctcat
    18901 ttgatacaac aagatttgct ctcctttaat gtcagtgaag aaaatagcga gaatggtgaa
    18961 tctttgccgt ggcatgcgcg tctgaaagaa aggttaggca gtataacaac acgtcgggcg
    19021 gtgacagcgc caacggcaac agagattatg accacttcta tccaggtgct ggagaaccgc
    19081 cttaaaagga accgcatggc aggtgatccg cccgatattc tgattcaacc tgtttgcccg
    19141 caaatatcta cgcttgattt ccatcgcgcg cacgctgcca ttgcggccgg acagctggca
    19201 gtggaaagga aaatggacga acttttgccg ttggtacgca ccaacatttg accagaattt
    19261 ttatctacac ttaagttaat tctgacaggc gcaggtggca atagcatgcc actattgagt
    19321 aaagccagtc aggggagaga acatgacgca gccattggtc ggaaaacaga ttctcattgt
    19381 tgaagatgag caggtatttc gctcgcttct ggattcatgg ttttcctcat tgggagcgac
    19441 aacggtactg gcggctgatg gggtggatgc ccttgagttg ctgggaggtt tcactccaga
    19501 cctgatgata tgtgatatcg cgatgccacg aatgaacggg cttaaactgc tggagcatat
    19561 acgtaacaga ggcgaccaga ccccagttct ggtgatatct gccactgaaa atatggcaga
    19621 tattgccaaa gcgttacgtc tgggcgttga agatgttttg ctgaaaccag ttaaagatct
    19681 gaatcgcttg cgcgagatgg tttttgcctg tctctatccc agcatgttta attcgcgcgt
    19741 tgaggaagag gaaaggcttt ttcgcgattg ggatgcaacg gttgataacc ctgccgcagc
    19801 ggcgaaatta ttacaggaac tacaaccgcc ggttcagcag gtgatttccc attgccgggt
    19861 taattatcgt caattggttg ccgcggacaa acccggcctg gtgcttgata ttgccgcact
    19921 ttcggaaaac gatctggcat tttattgcct tgatgtcacc cgagctggac ataatggcgt
    19981 acttgctgcc ttgttattac gcgcattgtt taacggatta ttacaggaac agcttgcaca
    20041 ccaaaatcaa cggttgccag agctgggcgc gttattgaag caggtaaacc atttactccg
    20101 tcaggccaat ctgccggggc agtttccgct attagttggc tattatcatc gcgaactgaa
    20161 aaatctcatt ctggtttctg cgggtctgaa tgcgacgtta aataccggcg aacaccaggt
    20221 gcaaatcagt aatggtgttc cgttaggcac tttaggtaac gcttatttga atcaattgag
    20281 ccagcgatgc gatgcctggc aatgccaaat atggggaacc ggtggtcgac tgcgcttgat
    20341 gttgtctgca gaatgagcaa acgataacgc ggactaaatt tgcattacct gctaatgtcg
    20401 gctggtggta ctatcgtcgc cattcgtata agtaattgtc ttaattatgc taactcgcct
    20461 ccttttcaga acttagcccc ttcggggtgc tgatatactg ggatgcgata cagaaatatg
    20521 aacacgttca aaacacgaac agtccaggag aatttaaatg gctgccatta atacgaaagt
    20581 caaaaaagcc gttatccccg ttgcgggatt aggaaccagg atgttgccgg cgacgaaagc
    20641 catcccgaaa gagatgctgc cacttgtcga taagccatta attcaatacg tcgtgaatga
    20701 atgtattgcg gctggcatta ctgaaattgt gctggttacg cactcatcta aaaactcgat
    20761 tgaaaaccac tttgatacca gttttgaact ggaagcaatg ctggaaaaac gtgtaaaacg
    20821 tcaactgctt gatgaagtgc agtctatttg tccgccgcac gtgactatta tgcaagttcg
    20881 tcaggggctg gcgaaaggcc tgggacacgc ggtattgtgt gctcacccgg tagtgggtga
    20941 tgaaccggta gctgttattt tgcctgatgt tattctggat gaatatgaat ccgatttgtc
    21001 acaggataac ctggcagaga tgatccgccg ctttgatgaa acgggtcata gccagatcat
    21061 ggttgaacag gttgctgatg tgaccgcata tggcgttgtg gattgcaaag gcgttgaatt
    21121 agcgccgggt gaaagtgtac cgatggttgg tgtggtagaa aaaccgaaag cggatgttgc
    21181 gccgtctaat ctcgctattg tgggtcgtta cgtacttagc gcggatattt ggccgttgct
    21241 ggcaaaaacc cctccgggag ctggtgatga aattcagctc accgacgcaa ttgatatgct
    21301 gatcgaaaaa gaaacggtgg aagcctatca tatgaaaggg aagagccatg actgcggtaa
    21361 taaattaggt tacatgcagg ccttcgttga atacggtatt cgtcataaca cccttggcac
    21421 ggaatttaaa gcctggcttg aagaagagat gggcattaag aagtaacatc catatcggtg
    21481 ttatccacga aacggcgttg aacaatcgac gccgtttttt tatagcttat tcttattaaa
    21541 ttgtcttaaa ccggacaata aaaaatcccg ccgatggcgg gatttttagc aattgcaatc
    21601 tacaaaagat tattgcttga tcaggaaatc gtcgagggat ttaccttgct catccattgc
    21661 ttttttgatt acagctggag tacggccctg gccagtccag gttttagttt cgccgttttc
    21721 gtcaacatag ctatattttg ccggacgctg agcacgttta gctttggtgc cggatttaac
    21781 ggcagcaagg ctattcagca gttcgttcgg gtcaataccg tcagcgatca gcatttcgcg
    21841 atattgctgc agtttacgag tgcgctcttc aacttcagca gcagccgcgc tttcttcttc
    21901 gcgacgttcg ttaacaacaa cttctaattt ttccagcatt tcttccagcg tttcaagtgt
    21961 acattctctt gcctgcgcac gaagagtacg gatgttgttc agaattttaa gtgcttcgct
    22021 cattgtagta atctcaaact tatattgggg tggtttgttg aggtaataat agagccttaa
    22081 attcagttgt gcaatagcca ggaatgtaag gaattcaaaa ttgttcttta ttttgtgccg
    22141 ccaataaata tcttttcata aaattagcca gaaaagacgc ggcatatagc cctatttaca
    22201 ccgatgattt cgcagcacgt gaggttaaaa cttcctgatt catgtcacat tttatgggga
    22261 gattatcgta ggctgacggc ctttcagtct tctgtattag ttgtgtttac gagaattccc
    22321 tattaagcga atgatgaaaa gtagaacagt cgcaataaga gcatggactt agtattgcac
    22381 tatctcctgg aggtcaacag agggctatta cttgcgcaac aggttaaaga ttgtgaatag
    22441 ttaccagcag tcatttaccc gcttataaca agcgaggcag ttgtaatgat agctcagaag
    22501 gattatgcaa ggcttcgtaa gggagaacgc atatacccac ttctgtgcat actgttgagc
    22561 tgaaaaactg acgaattatg ataaactcca gccaacttta tttcatatca ttgagggcct
    22621 gtggctgatg gcacagctat atttctacta ttccgcaatg aatgcgggta agtctacagc
    22681 attgttgcaa tcttcataca attaccaaga acgcggcatg cgcactgtcg tatatacggc
    22741 agaaattgat gatcgctttg gtgccgggaa agtcagttcg cgtataggtt tgtcatcgcc
    22801 tgcaaaatta tttaaccaaa attcatcatt atttgatgag attcgtgcgg aacatgaaca
    22861 gcaggcaatt cattgcgtac tggttgatga atgccagttt ttaaccagac aacaagtata
    22921 tgaattatcg gaggttgtcg atcaactcga tatacccgta ctttgttatg gtttacgtac
    22981 cgattttcga ggtgaattat ttattggcag ccaatactta ctggcatggt ccgacaaact
    23041 ggttgaatta aaaaccatct gtttttgtgg ccgtaaagca agcatggtgc tgcgtcttga
    23101 tcaagcaggc agaccttata acgaaggtga gcaggtggta attggtggta atgaacgata
    23161 cgtttctgta tgccgtaaac actataaaga ggcgttacaa gtcggctcat taacggctat
    23221 tcaggaaagg catcgccacg attaataaga atttctttac tgacagggtg agcagggcac
    23281 ttttatcctg tcagttcgtt ttacgcactt cttccgggct atataccctt ctcggcagtt
    23341 ttttaacgcc gctatacgcc tcacagggct cttaagcacc gacgttgact tgtgacctgt
    23401 aaagtacaat atccctgtgt ttaggcgtta tacatcgtcg caaatatgat gaaggctaat
    23461 gctgtcggtt tatggaaaag ttgctttggg taaacaaaaa atacggcccc agaagggcaa
    23521 tgccgttcac ttaagaggag cggcactatg tttcacagga taacgggttt ttgatatctt
    23581 aaccgacctc ggccttgatg gtcgggggcg ttttgttatg aacaccactt ccagagcacc
    23641 ccgaagatgc tccagtcgtt tcgggatggt ccctggtgac gccgtgtttc ccagctctat
    23701 catttctgat gcgatattct tccacgcagg cagtagccag tggcggttac aacccttctg
    23761 gttcagcgtt agcagcaggt cttcgctgta aaaaagctta tcaaacaacg taatagagtt
    23821 atccgggatg gtggcgagca tggagtgggc cagcacagtt tcgctctgcc ggtaaggtgc
    23881 ggtcacggca ttcagcagaa tgtgacttcc caggttcatt aaggccacca gacgcataac
    23941 cgggtaggcg ttctgccgct tagtggatgt gttggcagac ccataatatt cacgcagctc
    24001 gggtttatca ggtgtcctga actgtgcgcc atcaatggca aaaagttgca ggccgtgcca
    24061 gtcatccttc aggtaacgtt ccgcgcccct gtctgtgcgg tctggcggaa gagccattcc
    24121 actggggcgg cccccacgcg ctgacgcgct tgggtgacag cgctgcgggc cagcaggttc
    24181 atccccgctt cgccatccgc gctcaggttc agacggcgaa caacatcggt aattggctca
    24241 ttgcaccacc atccagataa ccatgtcccc cggtaaacga cggcggcgaa cggtcgcatg
    24301 agcagaaagc gtcaggcagt gttgtatcca ctcggtggga aggtgttctg caaatagttg
    24361 tgcagagggc ggaggcataa gcggatggtc actgaaatcg agcagatcat tgagaagtgg
    24421 cataagaaaa cggctccctg ttgtggaagc cgttatagtg cctcagttta aggatcggtc
    24481 aactaatcct taactgatcg gcattgccca gaaggggccg tttatgttgc cagacagcgc
    24541 tactgattaa gcggattttt tcgctttttt ctcagcttta gccggagcag cttctttctt
    24601 cgctgcagtt tcaccttcta cataatcacg accgtagtag gtatccagca gaatctgttt
    24661 cagctcggag atcagcgggt aacgcgggtt agcgccggtg cactggtcat cgaatgcatc
    24721 ttcagacagt ttatccacgt tcgccaggaa gtctgcttcc tgaacgccag cttcacggat
    24781 agatttcgga atacccagtt cagctttcag cgtttccagc catgccagca gtttctcgat
    24841 cttagcagca gtacggtcgc ccggtgcgct cagacccagg tggtcagcaa tttcagcata
    24901 acgacggcga gcctgcggac ggtcatactg gctgaatgca gtctgcttgg tcgggttgtc
    24961 gttcgcgttg tagcgaataa cgttacaaat cagcagggcg tttgccagac cgtgcggaat
    25021 atggaactgg gaacccagtt tgtgcgccat tgagtgacat acacccagga aggcgttcgc
    25081 aaacgcgata cccgcgatag tcgctgcact gtgaacacgt tcacgcgcta ccggattttt
    25141 agacccttcg tggtaggacg ctggcagata ttctttcagc agtttcagtg cctgcagagc
    25201 ctgaccatca gagaactcag atgccagtac agaaacataa gcttccatgg cgtgagttac
    25261 tgcgtccaga ccaccgaaag cacacaggga cttcggcatg tccataacca ggttggcgtc
    25321 gacaatcgcc atatccggag tcagcgcata gtctgccagc ggatatttct gaccagtagc
    25381 gtcgtcagtt acaaccgcaa acggagtgac ttcagaacct gtaccagaag tggtggtgac
    25441 agcgatcatt ttcgctttca cgcccatttt cgggaacttg tagatacgtt tacggatatc
    25501 cataaagcgc agcgccagct cttcgaagtg agtttccgga tgttcgtaca taacccacat
    25561 gatcttcgcg gcgtccatcg gggaaccacc acccagcgcg ataatcacgt ctggtttgaa
    25621 ggagtttgcc agttctgcac ctttacgaac gatgctcagg gtcgggtccg cttctacttc
    25681 gaagaagact tcagtttcaa cgcctgctgc tttcagtacg gaagtgatct gatcagcata
    25741 accattgttg aacaggaagc ggtcagtcac gatgagcgca cgtttgtggc catcagtaat
    25801 cacttcatcc agcgcgattg gcagggagcc acggcggaag tagatagatt tcggaagttt
    25861 gtgccacaac atgttttcag ctcgcttagc aacggttttc ttgttgatca ggtgtttcgg
    25921 accaacgttt tcagagatgg agttaccacc ccaagaacca caacccagag tcagggaagg
    25981 tgcgagtttg aagttataca ggtcaccgat accaccctga gacgctgggg tgttaatcag
    26041 gatacgagcc gttttcattt tctgaccgaa gtaagaaacg cgagccggtt ggttatcctg
    26101 gtcagtgtac aggcaagagg tatgaccgat accgcccata gcaaccagtt tctctgcttt
    26161 ttctaccgcg tcttcgaaat ctttagcgcg gtacattgcc agagtcgggg acagtttttc
    26221 atgtgcgaac ggttcgcttt catcaacaac ggtcacttca ccgatcagaa tcttggtgtt
    26281 ttctggtaca gagaagcctg ccagttcagc aattttatag gctggctgac caacgatagc
    26341 cgcgttcagc gcaccgtttt tcaggataac atcctgaaca gctttcagct ctttaccctg
    26401 caacagatag ccgccgtggg ttgcaaaacg ttcacgtaca gcgtcataaa cagagtcaac
    26461 aacaacaaca gactgttcag aagcacagat tacgccgttg tcgaaggttt tggacatcag
    26521 tacagatgca actgcacgtt tgatatcagc agtttcatcg ataacaactg gagtgttgcc
    26581 cgcgcctaca ccgatagctg gtttaccgga gctgtatgcg gctttaacca tgcccggacc
    26641 accagtcgcg aggatcaggt tgatgtctgg gtggtgcatc agtgcgttag acagttcaac
    26701 agaaggttga tcgatccagc cgatcagatc tttcggagca ccggcagcga tagcagcctg
    26761 cagaacgata tcagccgctt tgttggtggc atcttttgca cgcgggtgcg gggagaagat
    26821 aatggcgtta cgggtcttca gactgatcag cgatttgaag atagcagttg aagtcgggtt
    26881 agtggtcgga acgataccgc aaataatacc gattggttca gcgatagtga tggtaccaaa
    26941 agtgtcgtct tcagacagaa taccacaggt tttttcatct ttataggcgt tgtagatata
    27001 ttcagaagca aagtggtttt tgatcacttt atcttcgacg atacccatgc cggattcggc
    27061 aacggccatt ttcgcgagtg ggattcgagc atctgcagca gccagagcgg cggcgcggaa
    27121 gattttgtct acttgctctt gagtgaaact ggcatattca cgctgggctt tctttacacg
    27181 ctctacgagt gcgttaagtt cagcgacatt agtaacagcc ataatgctct cctgataatg
    27241 ttaaactttt ttagtaaatc atctgctcga atacgagagt atagtcagtg cggtgatgat
    27301 ttgcttaacc tatgaaaatc aaaagcttac tcgcgctcac actcactgtg atttactaaa
    27361 agagtttaaa cattagagtt attatctcta atgcgtcact tccaggtggc gtaagcaaga
    27421 ttactcactt ctgggtactg attacgtgat ccaaatcaaa ttttttgcaa agctgacacc
    27481 tttcagcatc gcttttcgcc attatagcta acagttaata aattgtagta tgatttggtg
    27541 gctacattag catgttttgc acaactagat aacaataacg aatgatagca attttaagta
    27601 gttaggaggt gaaaaatgct gtcaaaaggc gtattgtcag cgcgtctttt caaccttatt
    27661 tatggctaac attatccggc ttttgcttcg gagctaaccg tgattcagac cttttttgat
    27721 tttcccgttt acttcaaatt tttcatcggg ttatttgcgc tggtcaaccc ggtagggatt
    27781 attcccgtct ttatcagcat gaccagttat cagacagcgg cagcgcgaaa caaaactaac
    27841 cttacagcca acctgtctgt ggccattatc ttgtggatct cgctttttct cggcgacacg
    27901 attctgcaac tttttggtat atcaattgat tcgttccgta tcgccggggg catcctggtg
    27961 gtgacgatag cgatgtcgat gatcagcggc aagcttggcg aggataaaca gaacaagcaa
    28021 gaaaaatcag aaaccgcggt acgtgaaagc attggtgtgg tgccattggc gttgccgttg
    28081 atggcggggc caggggcgat cagttctacc atcgtctggg gtacgcgtta tcacagcatt
    28141 agctatctgt ttggtttctt tgtggctatt gcattgttcg ctttatgttg ctggggattg
    28201 ttccgcatgg caccttggct ggtacgggtt ttacgccaga ccggcatcaa cgtgattacg
    28261 cgtattatgg ggctattgct gatggcattg gggattgaat ttatcgttac tggtattaag
    28321 gggattttcc ccggcctgct taattaattc ctttcaaatg aaacggagct gccatgctcc
    28381 gtttacttcg tcattatttt tactttgttc ccgc
//
LOCUS       DAJTSL010000038        27907 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTSL010000038 DAJTSL010000000
VERSION     DAJTSL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27907
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.038"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..461)
                     /locus_tag="OO433_001482"
                     /pseudo
     CDS             complement(1..461)
                     /locus_tag="OO433_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
     gene            complement(639..1550)
                     /gene="gsiD"
                     /locus_tag="OO433_001483"
     CDS             complement(639..1550)
                     /gene="gsiD"
                     /locus_tag="OO433_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6050004.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1553..2473)
                     /gene="gsiC"
                     /locus_tag="OO433_001484"
     CDS             complement(1553..2473)
                     /gene="gsiC"
                     /locus_tag="OO433_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6050005.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILSSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2491..4029)
                     /gene="gsiB"
                     /locus_tag="OO433_001485"
     CDS             complement(2491..4029)
                     /gene="gsiB"
                     /locus_tag="OO433_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6050006.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILVH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4049..5920)
                     /gene="gsiA"
                     /locus_tag="OO433_001486"
     CDS             complement(4049..5920)
                     /gene="gsiA"
                     /locus_tag="OO433_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6050007.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLCVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5907..6872)
                     /gene="iaaA"
                     /locus_tag="OO433_001487"
     CDS             complement(5907..6872)
                     /gene="iaaA"
                     /locus_tag="OO433_001487"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6050008.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPAIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIGHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7076..8311
                     /gene="moeA"
                     /locus_tag="OO433_001488"
     CDS             7076..8311
                     /gene="moeA"
                     /locus_tag="OO433_001488"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6050009.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8311..9060
                     /gene="moeB"
                     /locus_tag="OO433_001489"
     CDS             8311..9060
                     /gene="moeB"
                     /locus_tag="OO433_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6050010.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHNDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEYDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9136..9798)
                     /gene="fsa"
                     /locus_tag="OO433_001490"
     CDS             complement(9136..9798)
                     /gene="fsa"
                     /locus_tag="OO433_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6050011.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKNPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9929..10828
                     /locus_tag="OO433_001491"
     CDS             9929..10828
                     /locus_tag="OO433_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6050012.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEIATALLQACHEAGIHTAVETC
                     LHVPWKYITPSLPYIDLFLADLKHIADAPFKQWTDGNAAKVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            10834..13266
                     /locus_tag="OO433_001492"
     CDS             10834..13266
                     /locus_tag="OO433_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6050013.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGNVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13412..14227
                     /gene="ybiV"
                     /locus_tag="OO433_001493"
     CDS             13412..14227
                     /gene="ybiV"
                     /locus_tag="OO433_001493"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6050014.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIAFDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14379..15644
                     /locus_tag="OO433_001494"
     CDS             14379..15644
                     /locus_tag="OO433_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6050015.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFSREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSTSRPEIYPIYWSQTQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     HEDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15885..17477)
                     /locus_tag="OO433_001495"
     CDS             complement(15885..17477)
                     /locus_tag="OO433_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6050016.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLMKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17696..18616
                     /gene="ldtB"
                     /locus_tag="OO433_001496"
     CDS             17696..18616
                     /gene="ldtB"
                     /locus_tag="OO433_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6050017.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18675..19793)
                     /locus_tag="OO433_001497"
     CDS             complement(18675..19793)
                     /locus_tag="OO433_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6050018.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKVLQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLASLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(19790..20257)
                     /gene="mntR"
                     /locus_tag="OO433_001498"
     CDS             complement(19790..20257)
                     /gene="mntR"
                     /locus_tag="OO433_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6050019.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20443..20571
                     /gene="mntS"
                     /locus_tag="OO433_001499"
     CDS             20443..20571
                     /gene="mntS"
                     /locus_tag="OO433_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6050020.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLILLSMLCDMVNNKPQQDKPSDK"
     gene            20843..22426
                     /gene="opgE"
                     /locus_tag="OO433_001500"
     CDS             20843..22426
                     /gene="opgE"
                     /locus_tag="OO433_001500"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6050021.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22475..22990)
                     /gene="ompX"
                     /locus_tag="OO433_001501"
     CDS             complement(22475..22990)
                     /gene="ompX"
                     /locus_tag="OO433_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6050022.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23043..23108)
                     /locus_tag="OO433_001502"
     CDS             complement(23043..23108)
                     /locus_tag="OO433_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050023.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23343..24230
                     /gene="rhtA"
                     /locus_tag="OO433_001503"
     CDS             23343..24230
                     /gene="rhtA"
                     /locus_tag="OO433_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6050024.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24529..25032
                     /gene="dps"
                     /locus_tag="OO433_001504"
                     /gene_synonym="pexB"
     CDS             24529..25032
                     /gene="dps"
                     /locus_tag="OO433_001504"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6050025.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25436..26182
                     /gene="glnH"
                     /locus_tag="OO433_001505"
     CDS             25436..26182
                     /gene="glnH"
                     /locus_tag="OO433_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6050026.1"
                     /translation="MKSVLKVSLAALTLVFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26321..26980
                     /gene="glnP"
                     /locus_tag="OO433_001506"
     CDS             26321..26980
                     /gene="glnP"
                     /locus_tag="OO433_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6050027.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            26977..27699
                     /gene="glnQ"
                     /locus_tag="OO433_001507"
     CDS             26977..27699
                     /gene="glnQ"
                     /locus_tag="OO433_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6050028.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
BASE COUNT         6883 a         7478 c         6918 g         6628 t
ORIGIN      
        1 tctgaagcca ccgaccacgg tcgtttcgat gggttaaaat cggcaaaaat aacccgccct
       61 gactcgtcga caagcggtcc atttacttat tcctgatgat aaacaacgat tttatcattc
      121 gccctccgtg aaaagagata atatttatta tcatcaagta ccagtcccac cgagtctaaa
      181 aatggcatca tttccatttt cagaatcacg gcacgttgaa tctcctctgt attttttaat
      241 aaatacagtg agtcctttaa tgtcagggcc gaacgttcaa tatctttaaa gaaatagcca
      301 acgtttttct gaataagttc aaagcggcta tcggcacgtt ttaccgcgcg ttccgatacc
      361 agttgatagt taatgatgaa aaaaagacca ataaataaca gtgctgccaa taaaaataaa
      421 attatcagcc ttatctttat cttttcgtat aaactcagca tgttaataca tttaacaaaa
      481 taactatctg aacgctgtgc cgctgcaaaa atagtccctt gctgcggctt tttaaccaaa
      541 ttacccgatt ctacaaaatc agcagaaagt tacaacactt gcttagggca ataatttcct
      601 gtgaggtgat taccctttca agcaatattc aaacgtaact atcctttaat tttcggatcc
      661 agcgcatcgc gtaaaccatc gcccaacaaa ttgaacgcca gtacggtcag aaaaatagcc
      721 agggccggaa aaacagcgac atgcggcgcg ataaccatat ccgctcgtgc ctcattgagc
      781 attgctcccc actctggtgt cggcggctgc gcaccgaggc cgagaaatga caggctggcg
      841 gcagagataa tcgaggtgcc aatgcgcatg gtgaaaaaca ccacgataga cgagacggtc
      901 ccaggcaaga tatgacgcaa taaaatggtc atatcgctgg caccaatact gcgtgctgac
      961 tcaataaagg tttgctgttt caacaccagc gtgttgccgc gcaccagtcg ggcaaacgcg
     1021 gggatggaaa aaatggcgac tgcgataatc acgttagcaa tgccgcttcc caacaccgca
     1081 acaacagcga tcgccagtaa aatacccggg aaggcaaaca gcacatcgca aatgcgcatg
     1141 atcagccgat cccaccagcc ttcataatat ccagccaaca agcccagcaa cgtcccgatc
     1201 gccgcaccga taaacacggc aaacacgccc gccgccagcg agatttgcgc accaaccagg
     1261 acacggctga aaatgtcgcg ccccagtgaa tcgacgccaa accagtgctg caaagaaggt
     1321 ccgttattca gattgtcata atcaaaataa ttttcggcgt catagggagc gatccagcgt
     1381 gcaaaaatgg ccaccacaat caataaaata acgaataatg cggcggtcat cgccatatgc
     1441 tgacggcgaa atcgtcgcca gaattcatgc cacggtgtac gcacctggtc aggtttgacc
     1501 agtggcatgg cgtttaacac cgcctgacgt cgccagttaa atagtcgcat ccttacttgt
     1561 acctgatagc cgggttaatg gcggcgtaaa gcacatccac cactaagttg ataagaataa
     1621 attccagcga gaaaagcaga atttccgcct gaatcaccgg gtaatcgcgc atttctacgg
     1681 agtcaacgag taagcgtcca agtccgggcc agttgaaaac tttctccacc acgatagaac
     1741 caccgagcaa aaagccaaac tgtaagccca tcatggtcac taccgggatc atcgcgttac
     1801 gtagcccgtg tttgaggaca acccaggttt cgctcacccc tttcgccctc gcggtacgca
     1861 tataatcttc gcttaaaaca tcgacaaacg acgcgcgggt aaagcgcgcc atcacggctg
     1921 ccactgccgc gccgagcgtc agggaggata aaatgtagtg ctgccaactg tctgctccca
     1981 cggtaggcag ccagcccagt tcaacggaga atacctgaat taaaagcatc cccagcgcaa
     2041 atgccggaaa cgagatcccc gacaccgcaa tggtcatact caatcgatcc ggccaacgat
     2101 tacgccagac ggcggcgata attcccgccg ccataccaaa tatcaccgcc cagaccatac
     2161 tggttatggt cagccacagc gttggcataa agcggctggc aatctcatcg gcaaccggac
     2221 gacgcgacac catcgacagg ccaaaatccc cctgcacagc attgctgata tagtgccaga
     2281 actggtgata cagcggctga tccaacccca gctgctgacg caccagttct ataacctgcg
     2341 catcagcttc gggcccggca atcaatcgcg ccggatcgcc gggcagcata tgcacaaata
     2401 aaaacaccag caccgagacg ataaacagcg tcggaatcaa ccccagtaag cgtttgataa
     2461 cgtaattaag cattccactc cctgcgttgc ttattgcaaa tccgcgtctt caaagctgaa
     2521 gccggtgtct ggcatgatcc aaaaaccggt caggttttta ctgtgtgccg acaccagttt
     2581 ttctaccacc agcgggatcc acggcgactc ttgccagatg atatcctgcg ccgccttata
     2641 caagcgggtc ttttccgccg gatcattagt tttcagtgcc tgagccagga agtcgtccac
     2701 ctgtttattg ctgtaaaatg cggtattaaa cagcgtcggt ggccagtttt gcgaggcgaa
     2761 cagtggcgat agcgcccagt ccgcttcgcc ggttgaagcc gaccagccag tgtagaacat
     2821 ccgcacgccg ctctcttttt gccctttacc ttcaacttct gccgcccgct gtccggcatc
     2881 catcgcggtc acctgggctt taatcccgac ctgcgctaac tgctgctggg taaattgcag
     2941 cactttctgc gcggtgctgt ggttatgtga cgaccacagc gtggtactga aaccgttggg
     3001 atatcccgcc tctttcagta attcgcgcgc tttcactgga tcgtaaggcc acggtttata
     3061 actttgcgcg taggcgatac ttggcggtac cacaccagta gctggcgttg catagcccgc
     3121 aaaggcaact ttcaccagcg ccggacggtt aatggcgtaa ttcagcgcct cacggacctt
     3181 cgggttatcg aacggctttt gcgtcacgtt catactgata taacgctgca taattgacgg
     3241 actggccatc aactcgatat ttttgttttt ctccagcagt gcggcctgct cgtagggaat
     3301 ggggaaagca aactgcgctt caccggtttg cagcattgcc gcgcgggtgt tgttatccgc
     3361 caccggacgc caggttatgc tgtccagttt gggcaatcct ggctgccagt aacccgcgaa
     3421 ttttttcacc ttcacaaaat cggtctgatt ccaggtatcc agttcatacg gtccggttcc
     3481 caccggatga aaaccaatct ccttgccata tttttccagc gctgccggtg aaatcatcgc
     3541 ggtcgccgga tggacaagaa tattaataaa cgctgagaac ggctgtttga gggtaatctt
     3601 taccgttgtc ggatcgatcg cctccgtttt ggcaatattc ttatacaggt tatagcgttt
     3661 aagatgattc gccggatcgc tggcccggtc cagattcgct ttcaccgccg cggcgttgaa
     3721 atcggtgcca tcctggaatt taattccttc ccgcaatttc acggtgtaag taatgccgtc
     3781 atcggaaacg gtataactct ccgccagcac gtttttcagt ttcatctctt tatccagacc
     3841 gaacagcccc tggtaaaacg atttcgctac ggcctgagat aacgtgtcat tcgcgtcata
     3901 cggatcgagc gtggtgaaat tcgatcctac cgccaccacc acatctttgg cagcgaatgc
     3961 acaagatgcc atcaacgctg tcgcaatgcc cagcgccact aacccactac ggtgtacagc
     4021 tcttgccatt ttattctccg cctgaatgtt atctacgcat gaatgcgtat tctgattgtg
     4081 gttgtgcgac gtaatgcccc ggcccgacgc attgcaacga gacggctgcc acctcttcgc
     4141 cacgcagatg aatattgctg ggaagatcgt ccgacagcag tacacgctgc ggtcgttgtc
     4201 gggacggttc agcgaccgga actgccgcca gtaatttacg cgtataagga tgctgcgggt
     4261 tttcgaagac cgcgcgccgt ggaccaattt caacaatttg cccgagatac atcaccgcca
     4321 cacgatgact aatccgctct accacggcca tatcgtggga gataaacaga tacgcaatgc
     4381 cgaaatcacg ctggagatcg agcaacaagt tgataatctg cccgcgaata gaaacatcca
     4441 gcgccgaaac ggcttcgtcg gcaatgatca cttttggatt caatgccaac gcgcgagcaa
     4501 tgcagatgcg ctggcgctga ccgccggaaa actcatgcgg gtaacgccag gcatgttcag
     4561 gtaacaggcc cacgcgctcc agcaaccacg caacgcgtgc agccgcgtct ttacctggca
     4621 ataaaccgtg tacacacagc ggttcgataa tcgaatcacc gatggtctga cgtgggtcca
     4681 gcgaagcgta agggtcctga aaaataaact gaatatcccg gcgcaatgcc tgaagtttgc
     4741 caggtgacaa ggtatcgatt cgctgaccgt taaagataat ttcgccgccc tgcgattcga
     4801 ccaggcgcag caacgcccgc ccggtagtgg atttaccgct gccagactcg cccaccagcg
     4861 atagcgtttc gccaggccag agatcaaaac tgactttctc aacggcatgc acttcccgcg
     4921 ttacgcgatt caacaaaccg ctgcgcaaag ggaaacgggt gacaagatta cgcactcgta
     4981 aaacaggttc gccatccacc accgttttct gctcgatggg gggttcctgt ttcgctggat
     5041 gttcaagcga tatcaatggg aaacgtcggg gataatctaa ccctttcatc gcaccaagtt
     5101 gcggaacagc agctaacagc gcacgggtgt aaggatgttg cggtgcatga aaaatctgtt
     5161 cgacggtacc cgtttccacc gcctcgccct gatacatcac cagtacccga tcggctatct
     5221 ctgccaccac gcccatatcg tgcgtgataa agataacgcc catcgacatc tctttttgca
     5281 atactttgat taattgcagg atctgcgcct gaatagtgac atccagcgcg gtggttggct
     5341 catcggcaat cagcaccgcc gggcggcatg acagcgccat cgcaatcatc actcgctggc
     5401 gcatcccgcc agagagttga tgcggataac gtgaaagaat ggtttgtgcc tcaggaatgc
     5461 gtacctgatc cagcatccgc ttcgcctcga ccatcgcttc ttcacgactg gcgttctgat
     5521 gcagacgaat tgattcggca atctgttcac ccacagtaaa taccgggttc agcgatgtca
     5581 tcggctcctg aaatatcatc gccatatccg caccgcgaac atggcgcatt tgtgcagcgc
     5641 tctgctcgct aagttcaatc acttcgcgac tgcgccgctg caacagcatt ttatcgcact
     5701 gtactaaacc gcccgcctgt tccaacaggc gcatcaatgc caacgcagtc actgacttac
     5761 cggagccgga ttcgccaaca attgccagcg tctcaccgcg ttgcagacta aaagagagat
     5821 tgcggaccgc agctattttc tgctggtcct gcataaaggc aatattcaga ttttcaaccg
     5881 ccagcacatt accggcatca agttcatcac tgtgtggcaa cggtgtcccc tttttcacgg
     5941 tagataccgg tggttggcgt atcgcctgcg tagccccagg cgcgatacat tccttcggtg
     6001 ttaaacggta gcgcgacatt cccttcatgg ccgatagcga ttaagccacc gctaccgcca
     6061 agcgcaggga gtttttccat tactacccgc tcgcaggctt ccgcgagact taatccgccg
     6121 taatccatta acgcggcgat gtcatatgcc gccagcgcgc ggatgaagac ttcgcccgtg
     6181 ccggtacaag aaaccgccac actggcgtta ttggcgtagc atccggcacc cactaaggga
     6241 ctatcgccaa ctcgtccggg taatttattg gtcattccgc ctgtggacgt ggctgccgcc
     6301 aaattgccgt ctaaatccaa cgccacggcc cccacggtgc ccattttctg tttttcatcc
     6361 agtggcgcac cgctatggtc gaggacggtt gccccttcct tgcgcgctgc cagtagttgt
     6421 tcataacgca aaggcgtgga gaaaatcgcc ggcgagacgc gctccatgcc acgagcaaac
     6481 gcaaaatttt ctgccccttc gccaatcatc atcacatgcg ggctttgctc catcaccagc
     6541 cgggcggcaa gaaccggatt acgcagatga ctaacgcccg ccaccgcacc ggctttcagg
     6601 gtgttaccat ccatcacaca ggcgtccagt tcatgggttt catcacgcgt aaagacagcg
     6661 ccaattccgg cgttaaacag tggacactct tccagcagac gcaccgcttc cgtcaccaca
     6721 tccagcgcac tttcgcccgc ttccagcatt ttctgcccgg tttcaacaat ggcagacaac
     6781 gcctcaatgt agcgtaattc ctgttgcaga ctcatctgcg cgcggctaat tgcacctgcg
     6841 ccaccatgaa ttgcaatgac tgctttgccc atagtattcg tccattaagg gtggctcgcc
     6901 acttttgcta taaatatcag aatttttatg aattactcat tcaatttttg aatatagaaa
     6961 gatggatatg gcatgtaaag gcaggagcca acgctacgcc cgaggtggca tacagtctgt
     7021 ggcgtttttc tgacataata ggcaaattcg attttgcctc cgcaggagtg ttttcatgga
     7081 atttaccacc ggattgatgt cgctcgacac cgcgcttaat gagatgcttt ctcgcgtcac
     7141 cccactgacc gcccaggaaa cgctgccact ggtacagtgt tttggtcgta ttctggcgag
     7201 cgatgtcgtt tcgccgcttg atgttccggg gtttgataac tccgcaatgg acggctacgc
     7261 ggtgcgttta gccgatattg cctccgggca accgctgccc gttgccggta aatcctttgc
     7321 cggtcagccg tatcatggtg aatggcctgc gggcacctgc attcgtatta tgaccggtgc
     7381 gccggtgccg gaaggctgcg aagcggtggt gatgcaggag cagactgaac aaacggacaa
     7441 tggcgtgcgt tttactgctg aagtgcgtag cgggcaaaat attcgccgtc gcggtgaaga
     7501 tatctctgca ggtgcggttg ttttcccggc gggaactcgc ctgactaccg cagagctgcc
     7561 agtaattgct tcactgggga ttgccgaagt tccggtgatt cgtaaagtgc gtgtagcgct
     7621 tttttctacc ggtgatgaac tccagttgcc cggtcagccg ctgggtgacg gccaaatcta
     7681 cgacaccaac cgtctcgccg tacacctgat gttagaacag ctgggttgcg aggtaatcaa
     7741 cttagggatt atccgcgacg atccccatgc cctgcgcgcc gcatttattg aagccgacag
     7801 ccaggcggat gtagtgatca gttccggcgg tgtttcagtg ggtgaagcgg attacaccaa
     7861 aacgattctt gaagagctgg gggagatcgc cttctggaag ctggcgatta aaccaggtaa
     7921 accgttcgcg ttcggtaaac tcagcaatag ctggttctgc ggcctgccgg gcaacccggt
     7981 ttcagcgacg ctgaccttct atcaactggt acagcctttg ctggcaaaac taagcggcaa
     8041 caccgccagc ggcctgcccg cgcgccagcg cgtacgcaca gcgtcccgcc tgaaaaaaac
     8101 gccaggacgt cttgatttcc agcgcggcgt gctgcaacgc aacgccgacg gtgaacttga
     8161 agtgacgacc accggacatc agggttcaca tatatttagc tcctttagtc tcggcaactg
     8221 ctttatcgtg ctggaacgcg atcgcggcaa tgtggacgtg ggcgaatggg tggaagtaga
     8281 accgtttaac gcgttgttcg gaggcctgta atggcggaac tcagcgatca ggagatgctg
     8341 cgctacaacc ggcaaatcat tctgcgcggt tttgattttg acggtcagga ggcgctaaaa
     8401 gattctcgtg tgctgatcgt cggcctcggt ggactcggct gcgcagcttc gcagtatctg
     8461 gcaagcgccg gtgtcggcaa cctgacgctg ctcgacttcg acacggtttc gctctcgaat
     8521 ctgcaacgcc agacactgca caatgatgcc acggtcgggc aaccgaaggt ggaatccgcc
     8581 cgtgacgccc tgacgcggat caacccacat atcgcgatta cgccagtcaa tgcactgcta
     8641 gatgacgcag aacttgcagc attgattgct gaatacgatc tggtgctcga ctgtacggat
     8701 aacgttgcgg tacgtaatca actgaacgca ggctgttttg ccgcgaaggt gccgctggtt
     8761 tccggcgcgg caattcgtat ggaagggcaa atcacggtct tcacttatca ggacggcgaa
     8821 ccgtgctatc gctgccttag ccgtttgttt ggcgagaacg cgttaacctg cgtagaagca
     8881 ggcgtgatgg caccgttgat cggcgtaatt ggttcgttgc aggcaatgga agcgatcaaa
     8941 ctgctggcag gttatggcaa accggccagc gggaaaatcg tcatgtacga tgcgatgacc
     9001 tgtcagtttc gcgaaatgaa actgatgcgt aatccggggt gtgaggtgtg cgggcagtaa
     9061 ttgccacctc caggccagat aagacgcggc actccgccac gtcatcagca atttatgagg
     9121 atgcagaacg tccggttaaa tcgacgttct gccaaacgct cccagccagt cctgttcaaa
     9181 cttcgccaca gcggcatcaa ccgccggata gctaatcatc tgttgtgcca catccagtgg
     9241 cagcgtaatt gattcacatc ctgccagtaa gcagtccagc gcctgacgcg ggtttttgaa
     9301 actcgctgcc agcactttcg cctgcggcgc atgcattttc aataactggt gtaagtcggt
     9361 cacagtctga atgccgctac cgccctgagc atcaatacga ttaacgtaag gcgcaacata
     9421 ttccgcacct gccagcgccg acagcagccc ttgtgctgcg ccatataccg cggttcccag
     9481 cgttggaatc ccttccgctt ttaacatctt aatagctgcc agcccctcgg cggtcaccgg
     9541 aactttcacc acgatatccg caataataga acgcaattta cgtgcgtcat tcaccatccc
     9601 ttcagcagtg gtagccatta cctgggcaaa cagacgcccc tgaccgccca tcgcttcatg
     9661 aagttgcgga agcacaacat ccagcggttt tttacccgcg gcgataatgc ttgggttagt
     9721 ggtcacaccc gccagcggaa aaatacgtga cagcgccttc actgcaacaa cgtctgaagt
     9781 atccagatac agttccataa ccatcctcaa aatgtacttt ttgtctgaat gtagactatc
     9841 acgggaaaac tctctgaaca gttgacgcac atcaatccaa ctttcattcg aaagtatttt
     9901 aatgtttgaa cgaaacagag aggcaactat gattttcaat attcagcgct actcgaccca
     9961 tgatggcccc ggtatccgca cggtcgtatt tcttaaaggc tgttcgctgg gctgccgctg
    10021 gtgtcagaac ccggaaagcc gcgcccgcac gcaggatctg ctgtatgacg cacgactgtg
    10081 tctggaaggc tgcgagctgt gcgctaaggc agcgccggaa gtgattgagc gcgcattgaa
    10141 tggtttgctt attcatcggg aaaagttaac cccggagcat ctgacggcgc taaccgactg
    10201 ctgcccgaca caggcgttaa ccgtgtgtgg ggaagtgaaa agcgttgagg agatcatggc
    10261 gaccgttctg cgcgataaac cgttttacga tcgcagcggc ggcggcttaa cgctttcggg
    10321 tggtgaaccc tttatgcagc cggaaatagc gacggcgcta ctgcaagcct gccacgaggc
    10381 aggcattcat actgcggtag aaacctgtct gcatgtgccg tggaaatata tcaccccttc
    10441 tctgccctat atcgatctgt ttcttgccga tttaaaacac attgccgacg cgccgtttaa
    10501 acagtggacc gacggtaacg ccgccaaggt gctggataac ctgaaaaaac tcgccgcagc
    10561 gggcaaaaaa atcattatcc gcgtgccgct gattcagggc tttaatgccg acgaaacctc
    10621 tgtaaaagcc attaccgatt ttgccgccga cgagctgcac gttggcgaaa ttcattttct
    10681 gccctaccac acgctgggca tcaacaaata tcacttactt aatctgccct atgacgcccc
    10741 ggaaaaaccg cttgatgcgc cagacctgct cgactttgcc cagcagtatg cctgccagaa
    10801 agggttaacc gcgaccttac gaggataaca accatgacca cactgaaact ggacacgctc
    10861 agcgaccgca ttaaagcgca caaaaatgcg ctggtgcata ttgtgaaacc gccggtctgt
    10921 accgagcgcg cgcagcacta taccgagatg tatcaacaac atctcgataa gccgatcccg
    10981 gtacgtcgcg cgctggctct ggcgcatcac ctggcgaatc gcaccatctg gatcaagcac
    11041 gatgagttga tcatcggcaa ccaggcaagc gaggttcgcg ctgcgccgat cttcccggaa
    11101 tataccgtct cgtggatcga aaaagagatt gatgatctgg cagatcgtcc cggtgctggt
    11161 tttgcggtga gcgaagagaa caaacgcgtt ctgcatgaag tgtgcccgtg gtggcgcggt
    11221 cagacggtac aggatcgctg ctacggcatg tttaccgatg agcaaaaagg tctgctggcg
    11281 accggaatca ttaaagcgga aggcaatatg acctccggcg atgcgcacct ggcggtgaat
    11341 ttcccgctgc tgctggaaaa aggtcttgat ggtctgcgcg agaaagtagc ggaacgtcgc
    11401 tcgcgcatca atctgacggt actggaagat ttgcacggcg aacagttcct gaaagcgatt
    11461 gatatcgtgc tggtggcagt cagcgaacac attgaacgtt ttgcagccct ggcgcgtgaa
    11521 atggccgcga ccgaaacccg cgaaagccgt cgcgatgaac tgctggcgat agcagaaaac
    11581 tgcgatctta tcgcccacca gccgccgcag actttctggc aggcgctgca actgtgttac
    11641 ttcatccagt tgatattgca gatcgaatct aacggtcact cggtatcgtt tggtcgtatg
    11701 gaccagtatc tctacccgta ctatcgccga gacgttgaac tcaaccagac actggatcgc
    11761 gaacacgcca tcgaattgct gcatagctgc tggctgaaac tgctggaagt gaacaagatc
    11821 cgctccggct cacactcaaa agcctctgcg ggaagtcctc tgtatcagaa cgtcaccatt
    11881 ggcggacaaa ctctggttga tggtcaacca atggacgcgg tgaatccact ctcttacgcg
    11941 atcctcgaat cctgcggtcg cctgcgttcc actcagccta acctcagcgt gcgttaccat
    12001 gcaggaatga gtaatgattt cctcgacgcc tgcgtacagg tgatccgttg cggcttcggg
    12061 atgccggcgt tcaacaacga cgaaatcgtg atcccggaat ttattaaact cggtattgaa
    12121 ccgcaggacg cttacgacta cgcagcgatt ggttgtatcg aaaccgccgt cggtggcaaa
    12181 tggggctatc gctgtaccgg catgagcttt atcaacttcg cccgcgtgat gctggcggcg
    12241 ctggaaggcg ggcgtgatgc cacaagcggc aacgtgttcc tgccacaaga aaaagcgttg
    12301 tcggcaggca actttaacaa tttcgatgaa gtaatggacg cgtgggatac gcaaatccgt
    12361 tattacacgc gtaaatcaat cgaaatcgaa tatgtcgtcg acaccatgct ggaagagaac
    12421 gtgcacgata ttctctgctc ggcgctggtg gatgactgta ttgagcgagc gaaaagtatc
    12481 aagcaaggcg gcgcgaaata tgactgggtt tctggcctgc aggttggcat tgccaacctc
    12541 ggcaacagcc tggcggcagt gaagaaactg gtatttgaac agggcgcgat tggtcagcaa
    12601 cagctggctg ccgcactggc ggatgacttc gatggcctga ctcatgagca actgcgtcag
    12661 cggctgatta acggtgcgcc gaagtacggc aacgacgatg atactgtcga tacgctgctg
    12721 gctcgcgctt atcagaccta tatcgacgaa ctgaaacagt accataatcc gcgctacggt
    12781 cgtggtccgg ttggcggcaa ctattacgcg ggtacgtcat ctatctccgc taacgtaccg
    12841 tttggtgcgc aaactatggc aacgccggac gggcgtaaag cccacacccc gctggcagaa
    12901 ggcgctagtc cggcctctgg tactgaccat ctcggcccta ctgcggtcat tggctcggtg
    12961 ggtaaactgc ctacggcagc gattctcggc ggcgtgttgc tcaaccagaa actgaatccg
    13021 gcaacgctgg agaacgaatc tgacaagcag aagctgatga tcctgctgcg caccttcttc
    13081 gaggtgcata aaggctggca tattcagtac aacatcgttt cccgcgaaac gctgctggag
    13141 gcgaaaaaac atccggatca gtaccgcgat ctggtagtgc gtgtcgcggg ttattccgcc
    13201 ttcttcaccg cgctctctcc agacgctcag gacgatatca tcgctcgtac tgaacatatg
    13261 ctgtaatctc ttcggcccgg ctgcggcgtc gccgggctaa atcattcaca tcatcaattt
    13321 catccttact ttcattcgaa atataatttg tgctctgcgt cacattgtta ttacactgtt
    13381 acggtttcat tttcgagcca ggagcacacc tatgagcgta aaagttatcg tcacagacat
    13441 ggacggtact tttcttaacg acgccaaaac gtacaaccaa ccacgtttta tggcgcaata
    13501 tcaggaactg aaaaagcgcg gcattgagtt cgttgtcgcc agcggtaatc agtattacca
    13561 gcttatttcg ttctttcctg agctaaagga tgagatctct tttgtcgcgg aaaacggcgc
    13621 actggtttac gaacatggca agcaactgtt ccacggcgaa ctgacccgac atgaatcgcg
    13681 gattgttatt ggcgagttgc taaaagataa gcaactcaat tttgtcgcct gcggtctgca
    13741 aagtgcatat gtcagcgaaa atgcccccga agcatttgtc gcactgatgg caaaacacta
    13801 ccatcgcctg aaagctgtaa aagattatca ggagattgac gacgtactgt tcaagttttc
    13861 gctcaacctg ccagatgaac aaatcccgtt agtgatcgac aaactgcaca tagcgttcga
    13921 tggcattatg aaacccgtca ccagtggttt tggctttatc gacctgatta ttcccggtct
    13981 acataaagca aacggtattt cgcggttact gaaacgctgg gatctgtcac cgcaaaatgt
    14041 ggtagcgatt ggcgacagcg gtaacgatgc ggagatgctg aaaatggcgc gttattcctt
    14101 tgcgatgggc aatgctgcgg aaaacattaa acaaatcgcc cgttacgcta ccgatgataa
    14161 taatcatgaa ggcgcgctga atgtgattca ggcggtgctg gataacacat ccccttttaa
    14221 cagctgacct ctctaccgga gccgtgcgct ccggttttcc cttcttttta catcctgtac
    14281 atcacatcaa atcattaaac ttgcattaac ttatttataa cttatattgt cacatgcaaa
    14341 tcataatggt ttcgaatgaa agttaatgag aggtggttat ggcttctact tttaccagcg
    14401 acacattgcc agccgatcac aaagcagcta tccgtcagat gaagcacgcg ctgcgggcgc
    14461 agcttggcga cgtccagcag atctttaatc agctaagcga tgacattgcc acgcgagtgg
    14521 ctgaaatcaa cgcactcaaa gcacagggcg atgccgtctg gccggtgctg tcttatgccg
    14581 atatcaaagc aggtcatgtt actgcagagc agcgcgaaca gattaaacgt cgcggttgtg
    14641 cggtgataaa aggccatttc tcccgcgaac aagcgctagg ctgggatcag tcgatgctgg
    14701 actatctgga ccgcaaccgc tttgacgagg tctacaaagg ccccggcgat aatttcttcg
    14761 ggacgctcag cacttcacgt cccgagattt accccatcta ctggtcgcag acgcaaatgc
    14821 aggcccgcca gagtgaagaa atggcgaatg cgcagtcgtt tctcaatcgt ctgtggacat
    14881 ttgaaagtga tggaaagcaa tggtttaacc cggatgtgag cgtcatctac cctgaccgta
    14941 tccgccgccg tccgcccgga acgacctcca aaggtcttgg agcgcatacc gactccgggg
    15001 cgctggaacg ctggctgctt ccagcgtatc agcgcgtttt cgccaacgtc tttaatggca
    15061 atctggcgca atatgatccc tggcatgcgg cacatcgtac ggaagttgaa gagtacacgg
    15121 tggacaacac caccaaatgt tccgtgtttc ggacattcca gggctggaca gcgctctctg
    15181 atatgctgcc tggtcagggg ttgctgcacg ttgtgcccat tcctgaagcc atggcgtacg
    15241 tactgttacg tccgctgctt gatgatgtgc cggaggatga actgtgcggc gtagcgcccg
    15301 gaagagtgtt gccggtatca gagcaatggc atccactgtt aattgaggcg ttaaccagca
    15361 ttccaaaact cgaggccgga gactccgtct ggtggcactg cgacgtcatc cattccgttg
    15421 cccccgttga aaatcaacag ggttggggca acgtgatgta cattcctgcg gcaccgatgt
    15481 gcgagaaaaa tcttgcctac gcgcacaagg tgaaggccgc actggaaaaa ggcgcatcgc
    15541 cgggcgactt cccgcacgag gactatgaaa caaactggga aggacgcttt acgcttgccg
    15601 acctcaacat tcacggtaag cgagcgttgg gcatggatgt ttgagagtta atggaagtga
    15661 attgatgtgg gattaagaaa atcgattcga cgttactcag attgctgaca agtgcgcttt
    15721 cttaatgcct gatgcggcgt taacgcctta tctggcctac aaagtcctgc agattcaata
    15781 tattacaggt actgcgtagg cctgataagc gtagcgcatc aggctatttt gcatttgtca
    15841 tcagccgccg tcaccccgac agtatcgggg tgacggataa tcacttactc gatcccttta
    15901 ctacgcaggt aatcttcgta attaccgcta aagtcgatca cgcgttccgg ggtgatttcc
    15961 agaatgcggg tcgccaggga gcttacgaac tcacggtcgt gtgaaacaaa gatcagcgtg
    16021 ccctgataca gttccagcgc catgttcagt gactcgatgg attccatatc caggtggttg
    16081 gtcggttcgt ccatgatcag aatgttcggc ttctgcatca ttaacttacc aaacagcatc
    16141 cgcccttttt ccccaccgga aagcacttta gctggctttt tgatgtcgtc ctggctgaac
    16201 agcaaacgac cgagaatgct gcgtaccgcc tgctcgtcat cgccttcctg cttccactga
    16261 ctcatccatt cgaacacggt cagatcattt tcaaactcat attcgtggtc ctgggcgtag
    16321 taaccaatgc gcgcgttttc agaccatttt acggtgccgc tgtccggttg cagatcgccc
    16381 accagcgttt tcatcagcgt tgatttaccg acgccgttgg tacccagtac cgcaagtttt
    16441 tcaccgacct ccagcagcag gttgagattt ttaaacagtg gaccgttatc aaaccctttg
    16501 gtcagacctt ccacttccag cgcgttacgg aacagtttct tatcctgttc aaaacggatg
    16561 aacgggttct gacggctgga ggctttcacc tcttccagtt tgattttatc aatctggcgc
    16621 gcgcgcgaag ttgcctggcg agatttcgag gcgttggcgc taaagcggct aacgaaagat
    16681 tgcaactcag caatctgcgc tttcttcttg gcgttatcgg ccagcagacg ttcacgcgcc
    16741 tgggtcgccg ccgtcatgta ctcatcgtag ttacccggat aaacgcgcag ctcgccgtaa
    16801 tccagatccg ccatgtgggt acagaccatg ttaaggaagt gacggtcgtg cgagatgatg
    16861 atcatggtgc tgtcacgctc gttcagcacc tgttccagcc agcgaatggt gtcgatgtcg
    16921 aggttgttgg tcggttcgtc gagcaggaga atatccggat cagcaaacag cgcctgcgcc
    16981 agaagcacac gcagcttcca gccaggagca acttcactca tcgggccgta gtgctgttcc
    17041 actggaattc ccacgccaag caacaattca ccggcgcgag cttccgcaga gtaaccgtcc
    17101 atttcgccgt atttaacttc cagatcggcc actttatagc cgtcttcctc actcatttcc
    17161 ggcaaagcat agatgcggtc acgctcctgc ttcacttccc acaactcttt atgccccatg
    17221 atcaccgtat ccagcacagt gaactcttca aaggcaaact gatcctgacg cagcttacca
    17281 atgcgctcgt tgggatcgag ggaaacgtta cccaaagtcg gctctaagtc gccgccgagg
    17341 atcttcataa aggtggattt accactaccg ttcgcgccaa tcaggccgta acggttgccg
    17401 ccgccaaatt tgacggaaat gttttcaaac aacggcttac tgccgaactg catggtgacg
    17461 ttactggaaa ctaacacagg cgtatcctga aaagagatat gacaaaccgc gcattatgcc
    17521 ataagtctga aagaagatcc tgctcctcgc ttaaggtcta cactcaagcc aaaggcaaaa
    17581 aatgtgattt cgtacacatc tgatttcact gtgagctgga atgaacttat aatgcgcttc
    17641 caatactcta atattctcaa cccaatggcc tgccaggcac aaaatctcgc ttaacatgaa
    17701 tatgaaattg aaaacattat tcgcagcggc cttcgctgtt gtcggctttt gcagtaccgc
    17761 ctctgcggta acttatcctc tgccaaccga cgggagtcgc ctggttggtc agaatcaggt
    17821 gatcaccatt cctgaaggta acactcagcc gctggagtat tttgccgcgg agtaccagat
    17881 ggggctttcc aatatgatgg aagcgaaccc gggtgtggat accttcctgc cgaaaggcgg
    17941 tactgtactg aacattccgc agcagctgat cctgccggat accgttcatg aaggcatcgt
    18001 cattaacagt gcagagatgc gtctgtatta ctatccgaaa gggaccaaca ccgttatcgt
    18061 gctgccgatc ggcattggtc agttaggcaa agatacgcct atcaactgga ccaccaaagt
    18121 tgagcgtaag aaagcaggcc cgacctggac gccgaccgca aaaatgcacg cagagtaccg
    18181 cgctgcgggc gaaccgcttc cggctgtcgt tccggcaggt ccggataacc cgatgggtct
    18241 gtatgcactc tacatcggtc gcctgtatgc tatccatggc accaacgcca acttcggtat
    18301 cggcctgcgt gtaagtcatg gttgtgtgcg tctgcgtaac gaagacatca aattcctgtt
    18361 cgagaaagta ccggtcggta cccgcgtaca gtttattgat gagccggtaa aagcgaccac
    18421 cgagccagac ggcagccgtt atattgaagt ccataacccg ctgtctacca ccgaagccca
    18481 gtttgaaggt caggaaattg tgccaattac cctgacgaag agcgtgcaga cagtgaccgg
    18541 tcagccagat gttgaccagg ttgttcttga tgaagcgatt aaaaaccgct ccgggatgcc
    18601 ggttcgtctg aattaatctt caaaacttaa agcaaaaggc ggactcataa tccgcctttt
    18661 ttatttgcca gaccctagtt ggccgggagt ataactaaca aaacatatcc caccaacgcc
    18721 gcccacaaca gcatcggtat tgaatagaga tggaaacgcc accagatgcg gcgatcgttc
    18781 gccatacgta gcgcaatcaa acttgccagc gatccgggta ataacccaaa gccacctacg
    18841 tttaccgccc atgccagtaa taaagacggc ggcacatagt tcagcaacaa tatggtactc
    18901 ggcacgttac tgatcacctg cgataaaccg attgccgtta accataaccc gggttcagat
    18961 agatgactca cgttacccaa cacgccttgc aacgctggaa gctgggtcag taaatggacg
    19021 tcgataaaca tcgccataaa caccagcagc agcgtccagt ccacactgag caccacgcga
    19081 cgtgccagca gcgcaaagcc tgccgccaca atcaccagtc cccacagctc ttgtttgaac
    19141 tccagcgccg ttagaaagac gatatacagc cccagacaac tccacaccag ccgcggtttc
    19201 cactccggtg tttgcacccc cgtatggtat tgcagcacct ttccagggaa acaacaccag
    19261 cacaggagca ggagcgtcag catcattgcg ccagccagcg gtgccatttg ggcaataaat
    19321 ccggcaaacg aaagaccaga acgtccccag ataagaatat tttgcgggtt gccaattggc
    19381 gtcagtagcg aaccagcatt gactgccagc gcctcaaaaa taatcagacg attaaccggg
    19441 atctcacaca gtctttttag cgtgatagtc agcggaacaa caataaacag cgcgacatcg
    19501 ttggtcagaa aggtagaaag cagcgccgcc gccagcacca taaacatcgc cagccgacgc
    19561 tccgtagcaa agcggcgcac cattttgcgc cccagcacat caaaataacc gcttaactcc
    19621 acacctttgg tcagcagcat caggccgctt aaggtgatga tggtgtgcca gtcgatagca
    19681 gcaggccagg atttcggtgc aaagggcacg aaaaagctta atccgatacc aacaagaatt
    19741 aataactgaa aaaaacgatc gccttgcagc gtgcgtaaaa aaggcaggct catttggcac
    19801 cgtgtttctg ggtaaacaaa cgaaaagcgt cgagcgtctc ttcactaaca tggtgctcca
    19861 tgccttccgc gtcgcgacgg gcgatttccg gactgacgcc caacaccaac aagaaatttt
    19921 cgactatctg atgtcgctcg cggctttcct gcgccagctt ctctccttct gccgttaaaa
    19981 acacgccacg ccaggggatc atttcaatca gccccatggt tgccagccgc ttaagcattt
    20041 tagccaccgt cggttgcgaa actcccagac gagcagccat gtccacctga cgagcttccc
    20101 ccacttccct gatcaagtca gaaatcagct caacgtaatc atcaataagc tcgcgccgat
    20161 gcgcctctct gacctggcgg aacccttcaa cgtgctcttc cacgttcact aactgcgtca
    20221 ctttttttgc tgttggcgta cctgcgcgac gactcattgt gcttcctcat tatggtgacg
    20281 catacatagc gttctcattt cgtgcgcaca ttgtaaacca gagttgcgaa ggtacaaaaa
    20341 attaacgttt tagcaatagc tatataatat agcctgtgct atatctgtat gtaatgcaat
    20401 catccctcaa ggatcgacgg gattagcaag tcaggaggtc ttatgaatga gttcaagagg
    20461 tgtatgcgcg tgtttagtca ttctcccttt aaagtacggt taatactgct ctctatgttg
    20521 tgcgatatgg tcaacaacaa accgcagcaa gataaacctt ccgataaata gcggcgtcgc
    20581 ggtacgccgc ttcactcctg ctttcatgca ggcataacgc gttttggcct gaaaaacccc
    20641 actttttgtc ggatttgcaa tccccttcgc aaaagatttg ttcgtcagta gttgacctga
    20701 acggcggctc gctctatctt cttgcagccc tgcgtatatt gcggctcgcg gatgcggacc
    20761 cctttccact cttcacgcac tcttgcaggt attgaccctt gacgccaggg taagcacatg
    20821 gcgtttgtta cgatagtggc atatgaattt aaccctcaaa gaatcgcttg ttacccgtag
    20881 ccgggtattt agcccgtgga ctgcgttcta ctttttacag tcgctattaa ttaacctcgg
    20941 cttaggttac cccttcagtt tgctctacac cgctgcgttt acggctattt tgcttttgct
    21001 atggcgaaca ttgcctcgcg tacaaaaagt tctggtcggt gtcagttcgc tggtggcggc
    21061 ttgttatttc ccttttgctc aggcctacgg cgcgcctaat ttcaatacat tgctggcatt
    21121 gcactccacc aatatggaag agtcgaccga aatcctgacg atttttccgt ggtacagcta
    21181 cctggtcggc ttatttattt ttgcgctcgg cgtaatagca atcaggcgaa aaaaagagaa
    21241 tgaaaaagcg cgctggaata ccttcgacag cctgtgtctg gtattcagtg tggcgacatt
    21301 ttttgttgct cccgtgcaaa acctggcctg gggtggcgta tttaaactga aagatactgg
    21361 ctatccggta tttcgttttg ctaaggatgt catcgtcaat aataacgagg tgattgaaga
    21421 gcaagaacgg atggcaaaac tttccggaat gaaagatacc tggacggtca ctgccgttaa
    21481 gccgaagtat cagacctatg tggtggtgat cggtgaaagc gcgcgtcgcg atgccctcgg
    21541 tgcctttggc ggtcactggg acaatacccc gtttgccagc agcgttaacg gtttgatatt
    21601 tgctgactac attgccgcca gtggctccac gcagaaatcg cttggcttaa cgctcaatcg
    21661 cgtagtcgat ggcaaaccac agtttcagga taactttgtc accctggcaa atcgcgcggg
    21721 cttccagacc tggtggtttt ccaaccaggg ccaaatcggc gaatacgata ctgctatcgc
    21781 cagcatcgcc aaacgtgcag atgaagtgta cttcctgaaa gaaggtaatt ttgaagcaga
    21841 taaaaacacg aaagacgaag cgttactgga tatgaccgct caagtgctgg cgcaagagca
    21901 ctcgcaaccg cagctgattg ttctgcatct gatgggctcg catccgcagg cctgcgacag
    21961 gacacaaggc aaatacgaaa cctttgtgca atcgaaagaa acgtcgtgct atctctatac
    22021 catgacgcaa acggacgatt tactgcgcaa gctgtacgat cagttacgca acagcggcag
    22081 cagcttctct ctggtttact tttctgacca cggtctggcc tttaaagagc gcggcaaaga
    22141 cgtgcaatac cttgcccatg atgataaata tcagcaaaat ttccaggtgc cttttatggt
    22201 catttccagc gacgataaag cgcatcgggt gattaaagcc cgccgctcag ccaatgactt
    22261 cttaggcttt ttctcgcagt ggacgggaat taaagcgaag gaaataaata tcaaataccc
    22321 gtttatatct gagaagaaag ccgggccgat atacatcacc aacttccagt tacagaaggt
    22381 ggattacaac catctcggaa ccgatatttt cgacccgaaa ccttaaaaac aaaaatccgc
    22441 cccgagaggc ggatttttta tatcaccaaa gtgattagaa gcggtaacca acaccggcaa
    22501 tccaggtgcc tacgtcaacg ctacgaatac ggctctgctc gtaagagaag tccagagcaa
    22561 cgttttccat cgggttgaac tgcagaccag caccgtagga gaaaccgtag tcgctggtgt
    22621 cgtgtttgta ggtcgggtat tcagtggtct ggaatttacc ataacccaca cccactacac
    22681 cgtagatgct tgcccagtcg ttaatgcggt aagccggacc agcagtgatg ccgtagtact
    22741 ggtttttgtt gtagtcacca gagcttgcag tacgactttt ctcggtgtaa gtgaaagaac
    22801 cgatcacacc cagcgggctg ttgtcttctt catagcggta tttcaggttg aaaccgccca
    22861 ttttgttcat ttggccctga gcgtcgctct gtgcgtaacc gccagttacg gtagaagtcg
    22921 cagctacgga agtacctgcg gtgaaagcca gaactgcggc cagtgctgaa agacatgcaa
    22981 tttttttcat aaccacctca aatgtgattc aaataagtcc taagttttaa atatatcaaa
    23041 aattaatggg aaactcttcg cgatttgtga tgtctaacgg gccatttcat gtaacagaac
    23101 gtttccatac accgctatcc atctaaattt aaatcacttt ttcagagaac tgcgtaagta
    23161 ttacgcatgt tttccctgtc attcatccag attattccta atcaccagac taatgattcc
    23221 atcaatcctg gcgcatttta gtcaaaacgg gggaaaattt tttcaacaaa tgctcaacca
    23281 gcattgggta tatccagtac actccacgct ttacttaagt ctagatattt gtgggagaaa
    23341 ggatgcctgg ttcattacgt aaaatgccgg tctggttacc aatagtcata ttgctcgttg
    23401 ccatggcgtc tattcagggt ggagcctcgt tagctaagtc actttttcct ctggtgggcg
    23461 caccgggtgt cactgcgctg cgtctggcat taggaacgct gatcctcatc gcgttcttta
    23521 agccatggcg actgcgcttt gccaaagagc aacggttacc gctgttgttt tacggcgttt
    23581 cgctgggtgg gatgaattat cttttttatc tttctattca gacagtaccg ctgggtattg
    23641 cggtggcgct ggagttcacc ggaccactgg cggtggcgct gttctcttct cgtcgcccgg
    23701 tagatttcgt ctgggttgtg ctggcggttc ttggtctgtg gttcctgcta ccgctggggc
    23761 aagacgtttc ccatgtcgat ttaaccggct gtgcgctggc actgggggcc ggggcttgtt
    23821 gggctattta cattttaagt gggcaacgcg caggagcgga acatggccct gcgacggtgg
    23881 caattggttc gttgattgca gcgttaattt tcgtgccaat tggagcgctt caggctggtg
    23941 aagcactctg gcactggtcg gttattccat tgggtctggc tgtcgctatt ctctcgaccg
    24001 ctctgcctta ttcgctggaa atgattgccc tcacccgttt gccaacacgg acatttggta
    24061 cgctgatgag catggaaccg gcgctggctg ccgtttccgg gatgattttc ctcggagaaa
    24121 cactgacacc catacagctg ctggcgctcg gcgctatcat cgccgcttca atggggtcta
    24181 cgctgacagt acgcaaagag agcaaaataa aagaattaga cattaattaa atttacattt
    24241 ctgcatggtt atgcataacc atgcagaatt tctcgctact tttcctctac accgtcttta
    24301 tatatcgaat tatgcaaaag catatttatt ccgaaaattc ctggcgagca gataaataag
    24361 aattgttctt atcaatatat ctaactcatt gaatctttat tagttttgtt tttcacgctt
    24421 gttaccacta ttagtgtgat aggaacagcc agaatagcgg aacacatagc cggtgctata
    24481 cttagtctcg ttaattactg ggacataaca tcaagaggat atgaaattat gagtaccgct
    24541 aaattagtta aatcaaaagc gaccaatctg ctttataccc gcaacgatgt ctccgacagc
    24601 gagaaaaaag caacagtaga gttgctgaat cgccaggtta tccagtttat tgatctttct
    24661 ttgattacca aacaagcgca ctggaacatg cgcggcgcta acttcattgc cgtacatgaa
    24721 atgctggatg gcttccgcac cgcactgatc gatcatctgg ataccatggc agaacgtgca
    24781 gtgcagctgg gcggtgtagc tctggggacc actcaagtta tcaacagcaa aaccccgctg
    24841 aaaagttacc cgctggacat ccacaacgtt caggatcacc tgaaagaact ggctgaccgt
    24901 tacgcaatcg tcgctaatga cgtacgcaaa gcgattggcg aagcgaaaga tgacgacacc
    24961 gcagatatcc tgaccgccgc gtctcgcgac ctggataaat tcctgtggtt tatcgagtct
    25021 aacatcgaat aaatccatcg ctgatggtgc agaactttag tacccgataa aagcggcttc
    25081 ctgacaggag gccgttttgt tttgcagccc acctcaacgc acttatttag tgcatccatc
    25141 tgctatctcc agctgattaa gtaaattttt tgtatccaca tcatcacaca atcgttacat
    25201 aaagattgtt ttttcatcag gttttacgct aaataatcac tgtgttgagt gcacaatttt
    25261 agcgcaccag attggtgccc cagaatggtg catcttcagg gtattgccct ataaatcgtg
    25321 catcacgttt ttgccgcatc tctaaaaatc aatgagttgc aaaactggca cgattttttc
    25381 atatatgtga atgtcacgca ggggatcgtc ccgtggatag aaaaaaggaa atgctatgaa
    25441 gtctgtatta aaagtttcac tggctgcact gaccctggtt tttgcggttt cttctcatgc
    25501 cgcggataaa aaattagttg tcgcgacgga taccgccttc gttccgtttg aatttaaaca
    25561 gggcgataaa tatgtgggct ttgacgttga tctgtgggct gccatcgcta aagagctgaa
    25621 gctggattac gaactgaagc cgatggattt cagtgggatc attccggcac tgcaaaccaa
    25681 aaacgtcgat ctggcgctgg cgggcattac catcaccgac gagcgtaaaa aagcgatcga
    25741 tttctctgac ggctactaca aaagcggcct gttagtgatg gtgaaagcta acaataacga
    25801 tgtgaaaagc gtgaaagatc tcgacgggaa agtggttgct gtgaagagcg gtactggctc
    25861 cgttgattac gcgaaagcaa acatcaaaac taaagatctg cgtcagttcc cgaacatcga
    25921 taacgcctat atggaactgg gcaccaaccg cgcagacgcc gttctgcacg atacgccaaa
    25981 cattctgtac ttcatcaaaa ccgccggtaa cggtcagttc aaagcggtag gtgactctct
    26041 ggaagcgcag caatacggta ttgcgttccc gaaaggtagc gacgagctgc gtgacaaagt
    26101 caacggcgcg ttgaaaaccc tgcgcgagaa cggaacttac aacgaaatct acaaaaaatg
    26161 gttcggtact gaaccgaaat aataacgcta cacctgtaaa gcgcactggc agttccctct
    26221 cccctctggg gagaggatta gggtgagggg cgcaaacccg ctccggggcc attaattacc
    26281 ctgaatttga ttatttacac cacggtaaca ggaacaacat atgcagtttg actggagtgc
    26341 catctggcct gccattccgc ttctgattga aggtgccaaa atgaccctgt ggatttcggt
    26401 cctcggtctg gctggcggtc tggtaatcgg attgctggca ggttttgcac gcaccttcgg
    26461 tggttggata gccaaccacg tcgcgctggt ctttattgaa gtgatccgcg gcacacctat
    26521 cgtcgtccag gtgatgttta tctatttcgc cctgccgatg gcgtttaacg acttacgcat
    26581 cgacccattt actgcggcgg tggtcaccat catgatcaac tccggcgcgt acattgcgga
    26641 aatcacgcgt ggtgcggtgc tgtctattca caaaggtttt cgtgaagcag gactggcgct
    26701 cggtctttca cgttgggaaa ccattcgcta cgtcatttta ccgctggcac tgcgtcgtat
    26761 gctgccgccg ctgggtaacc agtggatcat cagcattaaa gacacctcgc tgtttattgt
    26821 tatcggcgtg gcggaactga cccgtcaggg gcaagaaatt attgccggta acttccgcgc
    26881 ccttgagatc tggagcgccg tggcggtgtt ctatctgatt attaccctgg tgctgagctt
    26941 tattctgcgt cgtctggaaa gaaggatgaa aatcctgtga ttgaatttaa aaacgtctcc
    27001 aagcactttg gcccaaccca ggtgctgcac aatatcgatt tgaacattgc ccagggcgaa
    27061 gtcgtggtga ttatcgggcc gtccggttcc ggtaaatcga ccctgctgcg ctgcatcaac
    27121 aaactggaag aaatcacctc cggcgatctg attgtcgatg gcctgaaggt taacgatccg
    27181 aaagttgacg agcgcctgat tcgccaggaa gcaggtatgg tgttccagca gttttacctc
    27241 ttcccgcatc tgacggcgct ggaaaacgtc atgtttggtc cgctacgcgt gcgtggcgcg
    27301 aacaaagaag aggcggaaaa actggcgcgt gagctgctgg cgaaagtcgg tctggcagaa
    27361 cgtgcacatc actacccttc cgaactttct ggtggtcaac agcagcgtgt ggcgattgcc
    27421 cgcgcgctgg cggtgaagcc gaaaatgatg ctgtttgatg aaccgacttc cgctcttgac
    27481 ccggaactgc gccatgaagt gctgaaggtt atgcaggatc tggctgaaga agggatgacg
    27541 atggtgatcg tgacccacga aatcggtttt gccgagaaag tagcttcgcg gctgatcttt
    27601 atcgacaaag gccggattgc ggaagatggc aatccgcagg tgttgatcaa gaacccgccg
    27661 agccagcgct tgcaggaatt tttgcagcac gtctcttaat aagacacatt gcctgatgcg
    27721 ctacgcttat caggcctaca ggatatctgg caacttatta aaattgcatg aacttgtagg
    27781 acggataagg cgttcacgcc gcatccggca aaaaagcccg cacgttgtca gcaatctgct
    27841 taatatccct tcctcccttt cacccgaaag ggaggcacac cagattcctc tcatttaaaa
    27901 tcgcccc
//
LOCUS       DAJTSL010000039        27862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTSL010000039 DAJTSL010000000
VERSION     DAJTSL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.039"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..527
                     /locus_tag="OO433_001508"
     CDS             <1..527
                     /locus_tag="OO433_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6050029.1"
                     /translation="KYKIMANHHGIIFDEQRKRYIIGSITVSIDEMTNALDPVELAEI
                     INKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHN
                     GNDGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRN
                     GLMPLIRRRTMLQL"
     gene            893..1960
                     /locus_tag="OO433_001509"
     CDS             893..1960
                     /locus_tag="OO433_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6050030.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1957..4692
                     /gene="rbbA"
                     /locus_tag="OO433_001510"
     CDS             1957..4692
                     /gene="rbbA"
                     /locus_tag="OO433_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6050031.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSTLSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDMWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4692..5816
                     /locus_tag="OO433_001511"
     CDS             4692..5816
                     /locus_tag="OO433_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6050032.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5889..6163
                     /locus_tag="OO433_001512"
                     /pseudo
     CDS             5889..6163
                     /locus_tag="OO433_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6160..6519
                     /locus_tag="OO433_001513"
     CDS             6160..6519
                     /locus_tag="OO433_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6050033.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6639..7040)
                     /gene="nikR"
                     /locus_tag="OO433_001514"
     CDS             complement(6639..7040)
                     /gene="nikR"
                     /locus_tag="OO433_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6050034.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7046..7852)
                     /gene="nikE"
                     /locus_tag="OO433_001515"
     CDS             complement(7046..7852)
                     /gene="nikE"
                     /locus_tag="OO433_001515"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6050035.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDETVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRATEKV"
     gene            complement(7849..8613)
                     /gene="nikD"
                     /locus_tag="OO433_001516"
     CDS             complement(7849..8613)
                     /gene="nikD"
                     /locus_tag="OO433_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6050036.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8613..9446)
                     /gene="nikC"
                     /locus_tag="OO433_001517"
     CDS             complement(8613..9446)
                     /gene="nikC"
                     /locus_tag="OO433_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6050037.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSH
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVSGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9443..10387)
                     /gene="nikB"
                     /locus_tag="OO433_001518"
     CDS             complement(9443..10387)
                     /gene="nikB"
                     /locus_tag="OO433_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6050038.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQFFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10387..11961)
                     /gene="nikA"
                     /locus_tag="OO433_001519"
     CDS             complement(10387..11961)
                     /gene="nikA"
                     /locus_tag="OO433_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6050039.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKLSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKTPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12072..12659)
                     /gene="acpT"
                     /locus_tag="OO433_001520"
     CDS             complement(12072..12659)
                     /gene="acpT"
                     /locus_tag="OO433_001520"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6050040.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12714..13763)
                     /locus_tag="OO433_001521"
     CDS             complement(12714..13763)
                     /locus_tag="OO433_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6050041.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVTITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMIGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYACILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            13895..15112
                     /locus_tag="OO433_001522"
     CDS             13895..15112
                     /locus_tag="OO433_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6050042.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKSWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15116..15673)
                     /gene="dcrB"
                     /locus_tag="OO433_001523"
     CDS             complement(15116..15673)
                     /gene="dcrB"
                     /locus_tag="OO433_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6050043.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAENNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15746..16411)
                     /locus_tag="OO433_001524"
     CDS             complement(15746..16411)
                     /locus_tag="OO433_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6050044.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16632..16877
                     /gene="tusA"
                     /locus_tag="OO433_001525"
     CDS             16632..16877
                     /gene="tusA"
                     /locus_tag="OO433_001525"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6050045.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16979..19177)
                     /gene="zntA"
                     /locus_tag="OO433_001526"
     CDS             complement(16979..19177)
                     /gene="zntA"
                     /locus_tag="OO433_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6050046.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQSATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEYKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19251..19877)
                     /locus_tag="OO433_001527"
     CDS             complement(19251..19877)
                     /locus_tag="OO433_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6050047.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYF"
     gene            20018..20377
                     /locus_tag="OO433_001528"
     CDS             20018..20377
                     /locus_tag="OO433_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6050048.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20380..20649)
                     /locus_tag="OO433_001529"
     CDS             complement(20380..20649)
                     /locus_tag="OO433_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6050049.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20639..21235)
                     /gene="rsmD"
                     /locus_tag="OO433_001530"
     CDS             complement(20639..21235)
                     /gene="rsmD"
                     /locus_tag="OO433_001530"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6050050.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21385..22878
                     /gene="ftsY"
                     /locus_tag="OO433_001531"
     CDS             21385..22878
                     /gene="ftsY"
                     /locus_tag="OO433_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6050051.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFELQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22881..23549
                     /gene="ftsE"
                     /locus_tag="OO433_001532"
     CDS             22881..23549
                     /gene="ftsE"
                     /locus_tag="OO433_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6050052.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23542..24600
                     /gene="ftsX"
                     /locus_tag="OO433_001533"
     CDS             23542..24600
                     /gene="ftsX"
                     /locus_tag="OO433_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6050053.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24845..25699
                     /gene="rpoH"
                     /locus_tag="OO433_001534"
     CDS             24845..25699
                     /gene="rpoH"
                     /locus_tag="OO433_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6050054.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25970..27073
                     /gene="livJ"
                     /locus_tag="OO433_001535"
     CDS             25970..27073
                     /gene="livJ"
                     /locus_tag="OO433_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6050055.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27272..27655)
                     /gene="panM"
                     /locus_tag="OO433_001536"
     CDS             complement(27272..27655)
                     /gene="panM"
                     /locus_tag="OO433_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6050056.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6595 a         7229 c         7743 g         6295 t
ORIGIN      
        1 ccaaatacaa aataatggca aatcatcatg ggataatttt tgatgagcag cgaaaacgtt
       61 acattatagg atcgataaca gtctctattg atgaaatgac caatgctctc gatccggtcg
      121 aattagcgga aattattaat aaaaagcacc atgctgtaat taatggcaaa aagtttaaaa
      181 atagccgggc tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca
      241 gacctaaact tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg
      301 cacataacgg taatgatgga agagaatgta taggtgaaac ggggacagtt ttaggtctta
      361 acgcccgtga cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc
      421 ctggtgcagg gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa
      481 atggacttat gcctttgatt agaaggcgca caatgttaca gctctgaacc atagacaaca
      541 tgaatattaa tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg
      601 ttcctgcaac tgaacaggcg tgaaacgcga tccaacagga tggataccgt tttgtcagcc
      661 agttttatag atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga
      721 tgacaacatt actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg
      781 ctggtcgtat ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg
      841 gcggcagttg taacacgcgt cgtttgctta cagggattat ggcgggccga ttatggataa
      901 gagtaagcgc catctggcgt ggtgggttgt cggggcactg gcggtggctg ccgtcgtggc
      961 gtggtggctg ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat
     1021 tgaagcgacg gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt
     1081 gaaagaaggc cagtttgttc gcgaaggtga agtgctggcg aagatggata ctcgtgtgtt
     1141 gcaggaacag cgactggaag ccatcgcgca aatcaaagag gcacaaagcg ccgttgccgc
     1201 cgcgcaggct ttgctggagc aacgacaaag cgaaactcgc gccgcgcagt cgctggttaa
     1261 tcaacgccag gcagaactgg actccgtagc aaaacgtcat atgcgttccc gttcactggc
     1321 ccaacgaggg gctatttctg cgcaacagct ggatgacgac cgcgccgccg ctgagagcgc
     1381 ccgagctgcg ctggaatcgg caaaagctca ggtttctgct tctaaagccg ctatagaggc
     1441 ggcacgtacc aatatcattc aggcgcaaac ccgcgtcgaa gcggcacaag ccactgaacg
     1501 gcgcattgcc gcagatatcg atgacagcga actgaaagcc ccgcgtgacg gacgcgtgca
     1561 gtatcgggtt gccgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt
     1621 cgatctcagc gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctaaa
     1681 actgggcggt gaagcacggc tgatcctcga tgccgcgcca gatctgcgta ttccggcgac
     1741 catcagtttt gtcgccagcg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga
     1801 acggctgaaa ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca
     1861 tctggaatat gtcaaaacgg gcttgccggg cgtagcgtgg gtgcgggtga atgaagaact
     1921 tccgtggcct gacgacctgg tggtgaggtt gccgcaatga cgcatctgga actggttccc
     1981 gtcccgcctg tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg
     2041 aacaatatca ctctcgatat tccggcccgc tgtatggtcg ggctgattgg cccggacggc
     2101 gtcgggaagt cgagtttgtt gtcgttgatt tccggtgctc gtgtcattga gcagggcaat
     2161 gtgatggtgc tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ctgcccgcgc
     2221 atcgcctgga tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa
     2281 aacgtcgatt ttttcgcccg cctgttcggt cacgacaaag cggagcggga agtgcgaatt
     2341 aatgagctgc tgaccagcac ggggttagca ccgtttcgcg atcgtccggc agggaaactc
     2401 tccggcggga tgaagcaaaa acttgggctg tgctgcgcgt taatccacga cccggaactg
     2461 ttgatccttg atgagccaac aacgggggtt gacccgctct cccgcgccca gttctgggat
     2521 ctgatcgaca gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat
     2581 atggaagagg ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca
     2641 accggcagcg ccgaagagct gcggcagcaa acgcaaagcg ccacgctgga agaagcattt
     2701 attaatctgt taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat
     2761 caacctgaaa acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc
     2821 ttcgttgccg ttgatcacgt caatttccgc attccacgcg gggagatttt tggattcctt
     2881 ggttcgaacg gctgcggtaa atccaccacc atgaaaatgc ttaccggact gctgcccgcc
     2941 agcgaaggtg aggcgtggct atttgggcaa ccggttgatc caaaagatat cgatacccgc
     3001 cgtcgtgtgg gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa
     3061 aacctggagt tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcgcgagtg
     3121 gctgaaatga gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg
     3181 ccgctcggca ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg
     3241 ttaatcctcg atgagcctac ttctggtgtc gatccggtgg cgagggatat gttctggcag
     3301 ttgatggtcg atctctcgcg ccaggacaaa gtgactatct tcatctccac ccactttatg
     3361 aacgaagcgg aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gcttgccagc
     3421 ggtacaccgc aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc
     3481 gcctatttgc aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtggtacac
     3541 gacaccaccc acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc
     3601 cgcgaagcgc tggaactgcg acgcgatcca gtacgttcga cgctggcgct gatgggaacg
     3661 gtgatcctga tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt
     3721 gcggtgctcg accgcgacca gaccgtcagt agccaggcgt ggacactcaa cctctccggt
     3781 tcccgttact ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg
     3841 cgtgcgggcg atatcacggt ggcgattgag atcccgccca atttcgggcg cgatatcgcg
     3901 cgtggtacgc ctgtggaact cggcgtctgg atcgacggag cgatgcccag tcgtgccgaa
     3961 acggtaaaag gttacgtgca ggcaatgcac cagagttggc ttcaggatgt ggcgagccga
     4021 caatcgacac ccgccagcca aagcgggctg atgaacattg agacgcgtta tcgctataac
     4081 ccggacgtaa aaagcctgcc agcaattgtt ccggcggtga tcccgcttct gctgatgatg
     4141 atcccgtcaa tgctaagcac ccttagcgtg gtgcgggaaa aagagcttgg gtcgattatc
     4201 aacctttacg tgacccccac cacgcgtagt gaatttttgc ttggtaaaca gttgccatac
     4261 atcgcgctgg ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc
     4321 gtaccgcata aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatt
     4381 gccaccggaa tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc
     4441 ggaacggcga ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta
     4501 gcttcgctgg aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg
     4561 actatcgccc gcgggacgtt ctcgaaagcg ctggatctga ctgatatgtg gcaacttttt
     4621 atcccgttgc tgatagccat cccgctggtg atgggcttaa gtatcctgct gctgaaaaaa
     4681 caggagggat gatgcgccat ttacgcaata tttttaatct gggtatcaaa gagttgcgca
     4741 gtctgctcgg tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt
     4801 attcgtcagc gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata
     4861 tggatcaatc gcagttatcg aaccggatcg ttaacagctt ctatcgtccg tggtttttgc
     4921 caccggagat gatcaccgcc gatgagatgg atgccggact ggacgccgga cgctatacct
     4981 tcgcgataaa tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata
     5041 ttcaggtgaa cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc
     5101 agaatattat caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac
     5161 cgttggtatc gctggaaacc cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg
     5221 gcggggtgat ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg
     5281 cgctgatccg cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc
     5341 cgtttgagat catgatggcg aagatctggt cgatggggct ggtggtgctg gtggtatcgg
     5401 gattatcgct ggtgctgatg gtgaaaggtg tactgggcgt accgattgaa ggctcgatcc
     5461 cgctgtttat gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta
     5521 tggggacgat agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc
     5581 cgctgcaaat gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg
     5641 acattatgct gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc
     5701 ggggtgccgg attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg
     5761 cgtttttcac cattgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaggaa
     5821 gggaaacttg acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag
     5881 gacatgccat gagaaccaag gtacaggctt tacggaagaa acaaaaaata cattggatca
     5941 aatatttaaa actcctgttc ctcaagggat caagtggtct gatatagagt cactggttaa
     6001 agcattaggc ggagaaatta aggaaggaag aggttcgcgt tgtaagttca tactaaatat
     6061 gagcgttgcg tgtttccatc ggcctcatcc gtcgccagat accgataaag gcgctgtaga
     6121 aagcgtgcgt gactggttac taagtatagg ggtaaaacca tgatcaaact gaagacgcca
     6181 aactctatgg agattgccgg gcaaccagct gttattacct acgtgccaga actgaatgca
     6241 tttcgtggca aattcctggg cttgtcaggt tattgtgatt ttgtctctga cagcattcag
     6301 gggctgcaaa aagaagggga actttccctg ctagagtatc ttgaggattg taaagctgcg
     6361 ggtattgagc cgtatgcgcg tacagaaaag attaagacct ttacgttacg ctatccagaa
     6421 tcgctgagtg aacgccttaa taatgcggct gcgcagcaac aagtctctgt taatacctat
     6481 attattgaaa cactgaatga gcgtttgaat catttataaa ttttagcggc gtacattacg
     6541 cagaatatct gcctttacag gatctatcta tgcaactgaa cttaatttta taaattaagt
     6601 tcagttaata tcttttcttt cctcgtttca gaaataactc aatcttcctt cggcaagcac
     6661 tgcaaatgcc catgccgcac gccgcgctgg gcgataacgt catcggcaaa atgctgcacg
     6721 tcgcccatat cacctttcag cacggcgatt tccagacagt cgtcgtgatt gatatgcaca
     6781 tgcagcgtgg cgacggagag atcgtggtga tggtgctggg tggagacaat gcggctggct
     6841 aagtcgcgtt tttcgtgttc atacacatag gagagcaccg cgaaaccttg cgtgccgtgc
     6901 tgctgagtgg cctcctgggc cagggcgcta cgcagaatat cgcggatagc ttcggaacgg
     6961 ttgttataac cacgacgctg gctcaggctg tccagcgtct ccagtaaatc gtcatcaagc
     7021 gtgatggtga ctcgttgcat ttgcgttaaa ccttttctgt ggcgcgacgg cgcacgggga
     7081 atgcgggtaa taccgcgttt tgtagcacac gtccggcgtc agaggaaaag gttaatttat
     7141 ctcccaccgc ctgggtttcg acgatttgtc cgttgtccat caccatcacc cgctggcaaa
     7201 agcgttctac aagacgtaag tcgtgggtga tgaacaggca ggcggtgcca aactgttgtt
     7261 gcagcttttt cagcaggcga atgacgcccg cctgtaacac gagatcaagg ttagaaacgg
     7321 tttcatccag aatcagcaat ttcggttcga ccgccagcgc gcgtgccagg cagacgcgct
     7381 ggagctggcc gccgcttaac tgcggtgggc gtttgtcgag aacgctgtca tcgagatcga
     7441 ccgccttcag catttcgctg gcgcgcgcca gttgttcgga ttttttcagt gatagcaggt
     7501 ggcgcatcgg ttcacgcagg atctcgcgca cggttttgcg cggatttacg gcactgatgg
     7561 agtcctgaaa caccatctga atatcacggc ggaaggcttt acgttgggcg cgattaagtt
     7621 tcgccagcga ttcgccgcgc cagctaatat tcccttgtga gggagactct aagcctacca
     7681 gcagccgcgc gagggtgctt tttccgcagc cgctgcgtcc cagcagggcg acggtttcgc
     7741 cgcttttcag ggtcagggaa acgttattca gcaccgcctg atgttgatgt tttccgctaa
     7801 atccaccgtg ggcatagtga tgggaaaggc cgctgacgtt aagtaaggtc atgatgccag
     7861 ctccataccg tagagggcga gatgagcgga aaccaggctg cgcgtcaccg tatgtttggg
     7921 ggcgttaaac agcgtttcta catcgccctg ttcgacaatt ttaccttgtg acatcaccgc
     7981 cacgtcatcc gccagacgcg ccacaacgcc catatcatgg gtgaccagca gcattcccgg
     8041 cgcttgtttt tgcataatgc tttccagcag atcgaggatg cgcgcctgtg ctaccacgtc
     8101 gaggtcggtg gtcggttcat cggcgatgat aaacggtgat tcacacagca ccgccatcgc
     8161 aatcatcatg cgctgcaaca tgccgccgct catctcgaac gggtacagct tcagcacgcg
     8221 cgcggcgttt tccagcccca ccgcttctat ggcagcggta agcgtagcgt catcggcggg
     8281 tttccctaac gccaggcagg tttcccgcgc gtgggtgtgc atggtgtgta gcggattaaa
     8341 ggcgctgcgc gggttctgca tgatggtggc aattttgatg ccgcgcaggg cgcagggata
     8401 aaccgctttg ccatcggcta aaatttcccc cgccgtctgg cgaacgccag cgggcaaaat
     8461 gcccagcgtc gcggcgcagg ttaatgattt cccgctgccg ctaccgccga ctaacgccag
     8521 cacgcgcccg cgttgcaggg ttaacgatac accgtgtacc agcggctgcg cggcctgtag
     8581 cgcgatatta cgtagttcaa tctgttgcgg cattagtgtg cgtgctccgt caccagatga
     8641 gggtccagat gatcgcgcag tgcgtcaccc accaggttaa aggccatcac gctgataaac
     8701 agcgccagcc ccggccagaa catttgcagc ggctgggtcc agatatactg gcgcgcgtcg
     8761 ttaatcatca cgccccattc ggcggtcggc gcggtcacac cgaggccaag gaaagacatc
     8821 cccgagacgt gcagcatcat atggccgata tccagcgttg ccagcaccag cagcgaaggg
     8881 atcaccgcgc ctgccagatg atcgacaaac actcgcacat ggcccgcacc ggaaagccgt
     8941 gacgccagca caaactcgcg ttggcgtagt gaaatcacca ggctgcgcac catgcgtgca
     9001 taccacgccc agtgcgacag ggcgatggcg ataattacgt tggtcagccc ggtgccgagc
     9061 acgccaacca taaagaacga cagaatcgag gtcgggaagg tcataaacat atcggcgacg
     9121 cgcatggtgg cctgatcaac gcgcccgcca atcaacccgg cgctgccgcc aataaccagc
     9181 cctaatgtca gcaccagcag caggcaggcc attaccgaac cgagcgacac gcgggtcgct
     9241 gccatcagcc gcgagaaaat atcgcgacct aagtgatcgg tgcccagcca gtgctgcgca
     9301 tccggcgaaa gcaggtgcga cggcaaatca atcgcctggg ggtcatacgg cagccaccac
     9361 tggctggtga gcgcaatcag cgccagcagg gcgataatga tcagcgccag gcgtaccgac
     9421 cagcgggaag agaggaaaaa gttcacgcgt gcgctccttc atgacgacga atgcgcgggt
     9481 ccagcgcggc gttgagcaaa tcgacaatca aattacagac cacaaaaacc accaccatca
     9541 tcagcgtaaa gaactgaatc accggatagt cacggttaaa aatcgccgac accgcatagc
     9601 gcccgacgcc aggccaggca aagatgtttt cgataatcat cgtcccgcca atcaattcgc
     9661 caatgtgcat ccccacggcg gtgatcatcg gcagcgaggc attgcgcaga atatggcggc
     9721 gttcggtctg tttgtcattc aggccgcgta gccgcgccca ggtgacgtga cgctgaccgg
     9781 cgacgtccag catactggcg cgcagcagtc gcgcgttaat cgccagcgac ataaaggcga
     9841 tggaaaccgc aggcaaaatg atgtgctgcc agccgccgta gcccatcgcg ggtagccatt
     9901 gcagatacac cgaaaacgcc atcactagca aaaatgccag ccagaagttg ggcatcgaca
     9961 cgccaagaaa cgcgataaag cgcacggcga aatccggcaa acgatcgcgg tggcgcgccg
    10021 cccagatacc gagcggtacg gaagtgagca gaattaatac cagcgccgca cctgcaagtt
    10081 ccagcgtggc gggcaggaag ttcagcatat cgtccagcac cgggcgttgg ctggcgaatg
    10141 agataccaaa gtcaagatgc agcgccttcc acaaccaggt gccgtactgg acgtacagcg
    10201 gctgatccag tccgagcatg gtgcgggtag aggccagcat ctccggcgtc ggcggcaggt
    10261 tagacagacg caaataatcg agcgccgggt cgccggtacc gaggcgcagc atcagaaaaa
    10321 tgatcaccga ggcggcgagc accatcggga tcagcagcag aaagcggcgt aatacgtaac
    10381 gcaacattaa ggtttcaccg gtttaatctg ttcgaacgga atttcggtgg cgatcggcgc
    10441 gtaggggatg ttacccagct ccggttttga taccaccatc attgagatgt aactgatagg
    10501 cagataaacc gcctcgtcat gcagacgggt cagaatgtcg cgatacagcg cctgacgttg
    10561 cgtttcgtca tgggtcgcca gcacttcgcc gatctcttta tcaatcagcg gtttgtcggc
    10621 taatccttgc tgtgcctgga agtcagcgtg cgacggtacg cgcattgaac tcaggaaggc
    10681 gtgtggatcg tatggcgcgc cccaggtgcg gtggaaaatc atgccaaaac gaccgtcgcg
    10741 ctgacgagca tagatactgc tctcttcttc gccaatcagc gagacatctg cgccaatctg
    10801 gcgcatatca gcctgaatga tttccgccat cgatttgctt aacgcatcgg tgccgatgaa
    10861 cgacagttca atgcgcagcg gctgaccatt tttctcgcgg atgtctttgc ccgcaggcag
    10921 cgtccaaccg gctttttcca gcaacgcttt cgctttctgc ggatcgtact ggcgcggttt
    10981 caggccgagg ttggcgtagg gcacagaagg ggcaaacagg gtgtcggcga cctgctgggt
    11041 gccatacaac gcgttatcaa tcagcgattt tttgtttacc gcgtaattaa gagcttcacg
    11101 tactgccagc tcattggtgg gggttttggc ggtattaagc gccagcatca cggtttcgat
    11161 cggctgtgac agttgggtat ggtaagccgg gttctggcta aagcgggcga aggtatcgag
    11221 cggtaataac ccttcgtttc cgtacagcag gtcgatatcg ccagtttcaa acgccaccgc
    11281 gcgggtagtc gggtccggga tgacgttaaa ggtgatcttt ttaatcgctg gcttttcgcc
    11341 ccagtagttt tcgttacgga cgaagacatc gtactgattc agtttcgatt cctgcaaaat
    11401 ccacggtccg gtgccaatcg gcgctttaat tccgttcatg gtttcatggt ttttaaactg
    11461 cgagggagcg ataaaacgga aaggacgggg cagggccagt tcttgcagga aaggatagta
    11521 ggcgcttttc agggtaattt gcagctctgt tttactgagt gctttaacat caacaatctg
    11581 gtttgccagc tccagccagg cgtgacgttg acggttatcg agcactgcgc ggaagttttc
    11641 tgccgccgcc tcggcatcga acggttcacc gttggataat ttcacgtcat cacgcagggt
    11701 gaaggtccag gttttaccat cttctgaatg agtccagctt tttgccagcc acgggatcac
    11761 cgaaccgtct gcctgatatt tcaccaatgg ttcataaacc atgctctggg cgaacatctg
    11821 gttaggcgtg taaaggtgcg ggtttagtgg cccgacattc accggccagg cggtagtgat
    11881 ttcatctggt gcagcggcat ggacgataaa agacgcacaa gccagcagcg caaatagagt
    11941 gcggcggagt gtggagagca tgataacccc aatggattaa aatagatggc ggaaataagt
    12001 atgacgattt taagtattcg tcatactgat tacctgttct ggatcaatag atgggcagtc
    12061 gggtgggcgg atcagttaac tgaatcgatc cattgcacac tgtcggcggt gagcgtaaag
    12121 ggggtggggg tgcagatcgc aaggcttaaa ttttctagct gacaatggct gactgacagc
    12181 gaggagtgat aggtgctatc tacgctgacg atttgccagg cgctgccgcc acgctgttta
    12241 acgatggctt ctttgcgcgt ccagatgcgc cagaacattt ccagttgctg atcaggatgc
    12301 acggcgtcca tttcagcgtg ttccccgagg ctgaacacgg cgttcgccag ccagcgccag
    12361 ttggcgcgcg ggcgaatcac ttcgatatcg cagccgactt cgccttcatc gctcaacagc
    12421 agagcgatat cgtcaccgct atggcttaag ttgaaccata gcggcgtttc cggcgcgaat
    12481 gcaggtttgc cttgttcgcc atagatgatc tccggtagcg gggaaagcgt gtgcgaaagc
    12541 aatgcacgcc ccgccagcca gcgttcgcgt cgtggacctt gcggtgcttg ctcgcgtaaa
    12601 cccggtggca gtggagctgc gcttaaggtc gaaactttcc ccagaactat ccgatacata
    12661 tcagggccaa cgtttaatgg aaaatgaaag tgcgtatcgt atcacttgtc gcctcatccc
    12721 ggtaaccgac ttttcggtct gcctggcccc agtaaaatcg ccagtttgct accgcctttg
    12781 gtggtttcca tccagatttt acacacgctg gttaatggca ccgaaagtag catccctacc
    12841 gggccgagca gccatcccca aattaacaat gaaagaaata ccaccatggt ggacatcccc
    12901 aggcgatggc ccatcatccg tggttctaaa atattgccga tgaccatatg gaccactaaa
    12961 aacaatgcgc cgaccagaat acatgcgtaa acaccattaa acagcagcac ctgaatcatt
    13021 ggcggtacgg cggaaattac cgcgccgata ttgggcacgt agttgagcaa aaacgccagt
    13081 actgcccaca tcagcgcaaa ctgtacgccc attagcgcca gccccagcca gacgatgaca
    13141 cctgtccata aactgagtag cgtctttaac gccagataat gcgaaacgcc tttaagtgcg
    13201 cggtgtaatc ctgcgatgtg aatctgtggg ttattcagcg caaaacgcat tttgtaaggg
    13261 acgtggcgca cttcaaacag cataaaaact acggtcatca ccagcaaaag cacgctcgcc
    13321 attgccccgg aaagcccgat cattagcgct gtggtgaagg taaccacttt ttccgagtcc
    13381 atccgctgca gcattcgctc cggcgacata tgcaaattaa gaaaaggcaa catctcctgc
    13441 aatttaaaaa gtttgcgcgt cagctcctta ttaaacttcg gcagcataga gataaattcg
    13501 ttaaacgatg ccgccagtac gccgactagc gcggttagtg cgatcagcat caccaccact
    13561 acaatcgtaa tggtaacggg gcgttgtact ccccgacgaa taaaccaggt aacgagcggg
    13621 ttgaggacga tggcaaaaaa cagcgccagt aacagctgca caatgatatc tgccgctgcg
    13681 tgaatgcccg cgaggatcac taccagcgag gccaacttga gcagaatgtg catgcccgtt
    13741 ttatcgggtt gaggggtttc cattggggct tccttgtgac tttttgtgtt aagtgtagcg
    13801 ggagtcacgt cagcaatatt ctgattctta ctgctgattt tcacgtcagc acgtggtaaa
    13861 aatgaaacac tgttgtaaaa atgtggtgat cctcatgccc gaacccgtag ccgaacccgc
    13921 gctaaacgga ttgcgcctga atttgcgcat tgtctccatt gtcatgttta acttcgccag
    13981 ctacctcacc atcgggttgc cgctcgctgt attaccgggc tatgtccatg atgtgatggg
    14041 ctttagcgct ttctgggcag gattggttat cagcctgcaa tatttcgcca ccttgctgag
    14101 ccgtcctcat gccggacgtt acgccgattt gctgggcccc aaaaagattg tcgtcttcgg
    14161 tttatgcggc tgctttttga gcggtctggg atatctgacg gcaggattaa ccgccagtct
    14221 gcccgtcatc agcctgttat tactttgcct gggacgcgtg atccttggga ttgggcaaag
    14281 ttttgccgga acgggatcga ccctgtgggg cgttggcgtg gttggctcgc tgcatatcgg
    14341 gcgagtgatt tcgtggaacg gcattgtcac ttacggggcg atggcgatgg gtgcgccgtt
    14401 aggcgtcgtg ttttatcact ggggcggctt gcaggcgtta gcgttaatca ttatgggcgt
    14461 ggcgctggtg gccattttgt tggcgatccc gcgtccgacg gtaaaagcca gtaaaggcaa
    14521 accgctgccg tttcgcgcgg tgcttgggcg cgtctggctg tacggtatgg cactggcact
    14581 ggcttctgcc ggatttggcg tcatcgccac ctttatcacg ctgttttatg acgctaaaag
    14641 ttgggacggt gcggctttcg cgctgacgct gtttagctgt gcgtttgtcg gtacgcgttt
    14701 gttattccct aacggcatta accgtatcgg cggcttaaac gtagcgatga tttgctttag
    14761 cgttgagata atcggcctgc tactggttgg cgtggcgact atgccgtgga tggcgaaaat
    14821 cggcgtctta ctggcggggg cagggttttc gctggtgttc ccggcattag gtgtagtggc
    14881 ggtaaaagcg gttccgcagc aaaatcaggg ggcggcgctg gcaacttaca ccgtatttat
    14941 ggatttatcg cttggcgtga ccggaccact ggctgggctg gtgatgagct gggcgggcgt
    15001 accggtgatt tatctggcgg cggcgggact ggtcgcaatc gcgttattac tgacgtggcg
    15061 attaaaaaaa cggcctccgg aacacgtccc tgaggccgcc tcatcatctt aaaacttact
    15121 gaataaccag cgtattaatg atgttttctg cggtggtctg cgctttttgc tgatcgtcag
    15181 cgggcagcgt aatttgcatg gtcagcagtt gattacccac gttacccaga ataacggaag
    15241 agtacgccgt ctggcctttc gcggagataa tactgtctaa ctgctgcatt ttgtgacctt
    15301 tcagctcaat ggctttattg gttaccactt gcagctgcgg atcgcggcta cgttgctgat
    15361 cttccagacg cttcgccagc accgccagat cttctttcgg atcatcgccc atgatgacga
    15421 tgactgcttt ctgcccggtg gcgtcggacc agacatgcat gttattggcc tgcgttccca
    15481 gcttaccgct ctggtcggtc atatccgctg gcagcgagaa acttaacttg ccatcaagca
    15541 ggttgacggg attcccggta gcgttatttt ccgcgaccga accctgcgcc gtagcgttag
    15601 tgtctttatc atcacaggcc gcaagcccca taaccagcag gccaattccg acatatttaa
    15661 ccagattgcg cattgacttc ttcctttcga taaacggcca taacggctca ttcatccatc
    15721 ttatcacaac tctgataacg aacctttaac tcgcctgcaa agcgttgatt tcggatttat
    15781 ctgccagtct tttcaacagc atattgagta atacgccata cattggcagg aagaaaacga
    15841 tactgattaa cactttgaaa cagtaatcga ccagcgcgat ttccatccag tgttcagcca
    15901 taaaggcatc cgggctacgc cagaaggcaa tgaagaaaaa ggccagcgtg tcgctgacgt
    15961 taccgaacag tgtggacgct gtcggtgcca gccaccagcg gcgactctga cgcaggcggt
    16021 taaaaacgtg cacgtcgagg atctgtccca gcgcgtaggc catgaaactg gcggtggcga
    16081 tacgggcgac aaacaggttg aagtgggcga gtgcgccgaa tccctgccag gaacccatat
    16141 agaatagcga cgagatgacg taggagatca gcaacgccgg gatcatcacc gcaaagatga
    16201 tacgacgggc cagcggtgcg ccaaaaatac gcacggtcag gtcggtagca aggaaaataa
    16261 acgggaagct aaacgcgccc caggtggtat ggaaacccaa aatggagacg ggaagctgaa
    16321 ccagatagtt actggaggtg atcaccagca gatgaaataa cgataaccag aacaacgcct
    16381 tatagcgttg agtttgcgag aaaacgttca tattgtacct ttttgattaa ccattggggt
    16441 gagggaaccc aatacgtacg acacgtctgt taccttaaca taataacgtg cttacccttt
    16501 tttcgagccg ccgcatgata ctgctttacg ttgacaatgc aatggttaat tttcacgcaa
    16561 tcgttaacct ggtttgctta cggactcaca gggcgtaaac tagcgccgtt tttttatgtg
    16621 atgagaagaa aatgaccgat ctcttttcca gccctgacca cacactcgac gcacttggcc
    16681 tgcgctgccc ggaacccgta atgatggtgc gcaaaactgt gcgcaatatg cagcccggcg
    16741 aaacgttgct gattatcgcc gacgatccgg ccactacccg cgatattcct gggttttgta
    16801 cctttatgga acacgaactg gttgcgaaag agacggatgg actgccttat cgttatttga
    16861 ttcgtaaagg cggttgatgg gggctgattg gcttcgatgc cgccttttcc cctcacccta
    16921 accctctccc cagaggggcg aggggaccga tcgcgctcaa tgttgcgatc ggtttgcctt
    16981 atctcctgcg caacaatctt aacgcattcg ctgtcaccag caccgtcgca cccgtatctg
    17041 ccagcaccgc cagccatagt ccggtcatcc ctaaaagcgt ggtgacgagg aagatccctt
    17101 tcagtcccag cgcaatggtg atgttctggc ggatattggc gtgagtggcg cgtgccagtt
    17161 caatcatttg caccaggccg cgcaggtggt tatgggttaa tgctgcatca gcggtttcca
    17221 gcgccacgtc tgtgccactg cccattgcaa tcccaatggc ggcagctttc atcgctggcg
    17281 cgtcgttaat accgtcaccg accatcgcca gcggcgcatg ttgattcagc tcggtcaccg
    17341 ctttgacttt atattccggc aacaggcccg ctttaaattc cagccccagc tccccggcaa
    17401 ttgccgccgc tgcgcgtgga ttatcgccgg tgaggatcac ccctttgacg cccagcgcgt
    17461 tcagttcact gatggcagtt gcagcatcgg cgcgcagggt atcctgcaat gcaatgatac
    17521 ccagcacgtc atcattacgt actaccagca caaccgtttg cccggcgctt tccagttcat
    17581 taatcaaacc agcaaatgca tcagcgggat gtttcccggc agcgcatatc aacacgcgct
    17641 caccgttaac ctgcgcttca atgccagacc cgaccagcgc ccgctgtgat tcggcggcgg
    17701 gaatggcgag tgcagcaacc tgtgcttcgc gcacgatggc ctgcgccagt ggatgcgtcg
    17761 cgctttgctc gaccgccgcc gccagtgtca gcagttcaga ttcactaata cccgttgccg
    17821 gatgaatcgc ggtaacgcgc ggtttaccga cggtcagcgt accggtttta tcaaacgcca
    17881 cctgagtaac acgacctagt tgttccagcg ccgctccgcc tttaatcaac gccccacgac
    17941 gcgctgctgc cgccagcccg gaggtaatcg ccgcgggcgt ggagataact aacgcacacg
    18001 ggcagccaat cagcagcagc gtcaggcctt tataaatcca ctcctgccag ctggcggcaa
    18061 acagcagcgg tggcaccagc gtcaccagca gggcgacggc cataatcgct ggcgtataaa
    18121 tacggctgaa acggtcgata aaccgctcaa tgggggcgcg gcgctcttcg gcttcttcga
    18181 tcagtttcag aatccggtca atggcgctgg cacccggttc tgacagcact tccagcgtta
    18241 ccagacggtc tacgctggtg gctcctgcag gaactttatc gcccgtcgcg cgctccaccg
    18301 gaatggattc tccggtcagg gcgctttcat caaaactggc aaacggtgag agcagtttac
    18361 cgtcggcagg caaacgccca cctgcggcga cttcaatcac atcgccaggc cgcaggctgt
    18421 taatcgccac ctcttcccgc tcaccgttac gcaggcgcgt ggcggtttct ggtttcagcg
    18481 ccattaacgc gctaacgccc tgacgcgcgc ggctggcggc ccagccttcc agtcgttcac
    18541 caatcaaaaa cagcagcaac accatcgcag cttcagccgt tgcgccaata aacagtgcac
    18601 caatagcggc tacgctcatt aaggtttcaa tggcgaagta gctgccggat ttgatcagcc
    18661 gtaatgcctg atgagcaatc gggtacagcc caaccagcgt ggtcgcgata aacgccagtt
    18721 gcccgaacgg atgattgaac tgctccagac cccagctgat tgccatcatc acgattagtg
    18781 taatcagcgg cagattctct ttcaggcgcg atgcttgtgg ctcgtcggcg gcctgctcat
    18841 cgcgcaggga atagcccgct ttttgcaccg cagattcaac ttgtgcacga atgtcattgt
    18901 cggcatcaac caccagtttt tcggtggcga acaacacctg cacctgattc acacctgcaa
    18961 gctggcgcac ggcattttct accttgcgcg cacaggcggc gcagtccatg ccactgactt
    19021 tccagctata gcgggtgccg gagacgtttt cagagagcgt tggcgagctg gagcatgcgc
    19081 cgtcgcagca acagtcgttg gcgttctgta ccgtggttag cggtttgaac gcagcaaatt
    19141 gaggggcttt cttgccgtga ttgtcaggtg tcgacatggc atactccggt taagtttttt
    19201 ctcattaacc gaaggataca ctctggagtc gactccagag tcaagtttta tcagaaatac
    19261 agtgagcgga cgatgaggaa gtgaccggca aagtagcagg ccgcagcaat cgcgttatcc
    19321 gcacggaagc gtcggcgata gtggctcccc agccagacaa agttactgat aaacagcaac
    19381 gaagcgccaa caaacgcaga gagcgccgga gcggtcggac ggaagaacca cagttcacct
    19441 gccagccaca ccatcaccag cgtcatgccg ataaacgtgc agataggcca acgatactct
    19501 tccagccgcg tccagataat cgccagtaac agcgcaccca gaaccagcaa caccagtggc
    19561 agaggccaga agaaagagag cgtcatctga ctggcgaaat agatggtgta cagcaggtgc
    19621 gagaggaaaa acgcgccgat ggcgtacatc agacgttgac gtggcaacag ggttagcgca
    19681 tcgcccagca gtgaggcgca cagccctgcc agcaccagat agctaatggc gtcgaacatc
    19741 ggcgcttgcc aggccagtaa cagcaggaga agaagggtta acggtttaaa cacccagcgt
    19801 tgccaggttg gcccacgata cgatgcatcc acagatagcc atgcggaaag acagacagcg
    19861 ataaacgacc aaagcatctt agttccttga attcgttatt ttttcgtaag cggaatgcct
    19921 acggtcttgc ttccagttta gtggcgaggg tggacggatg acaacaccat aactagcagg
    19981 gtatgcttat ttccgcattt tccgatgagg gataaagatg agcaaaccac ctcttttctt
    20041 tattgttatc attggcttaa ttgtcgtcgc cgcatcgttt cgttttatgc aacagcgacg
    20101 ggaaaaagct gataatgata tggctccgct ccagcaaaag ctggtggtgg tgagcaacaa
    20161 gcgggaaaaa ccgattaacg atcgccgttc gcgccagcag gaagtgactc cggcaggcac
    20221 cagtatgcgc tatgaggcaa gcttcaaacc gcaaagcgga ggaatggagc agacgtttcg
    20281 cctcgacgcc cagcagtacc atgccctgac agtgggcgat aaaggtacgc tgagctataa
    20341 aggaacgcgc tttgttagct ttgttggcga acaataaggt tattttttaa ctttaaattt
    20401 cttctgccag accagcagtt caaacacgcc gaaaaggaaa atccgcacct tttcggcggt
    20461 ggtcatctgc gggccatctt tcggtaaagt ggatttcaat agcgccagct gcataccatg
    20521 catcagcacg gtaaaaatca gcgccacgtt aacgaaaata ttcagcgggc gcgggaaggg
    20581 atgcaccaga ttgaggatta aaaatcccca aacgcagagc attaacaaac gaccaatatt
    20641 aatcagcatc actttctcct tgtgcttcgc gttgatacag ccgataagcc acctgacccg
    20701 ccactttttc ccgatgtaat gaccagtttg ccggaacagt gggcagaccg ttttcgactt
    20761 cgctttcgac ataaatcagg gcttcgtcag ccagccagcc gttatcttcc agtaaattta
    20821 tcgtctcttc taacaagcca cggcggaacg gtggatcgac aaacacgata ttatgcggtg
    20881 tacctttttg cgccaggaat gacatcgcgt tgctgttcac cacgcgtgca ttgcctgctt
    20941 ttagtgtcgc cagattctta attaactgct gagaaaccgc gcgatccatc tcaatcaacg
    21001 tggcccccgc agcgtagcgc gataacgctt ccagtcccag cgcgccgctc ccggcgaagc
    21061 aatccagaca ttgggcgtca acaatgaccg gagccagcca gttaaacaat gtttcgcgta
    21121 cgcggtcggt ggtggggcgc agacctgggc tatcaggaac cgggagttta cggcctcgcc
    21181 actgcccgcc aataatgcgg atttggccgc tgccggaatg attcggtttt ttcatgataa
    21241 attgctcaat ccgccagata acacatactt gcaggcggtg atgtgaatta agttaagtga
    21301 tagactattt catcattttt ttagctgcta tgtacatagc gttaacgctg tgccatgaag
    21361 caacagcgag gagtgtagtc gcaaatggcg aaagaaaaaa aacgtggctt tttttcctgg
    21421 ctgggctttg gtcaaaaaga gcagaccccg gaaaaagaga cagaagttca gaatgaacaa
    21481 ccggttgtag aagaaatcgt tcaggcgcaa gagcctgcga aggcttctga acaagccgtc
    21541 gaagagcaac cgcaggcgca tactgaagcc gaggcggaaa cttttgctgc cgacgttgtg
    21601 gaagtcactg aacaggttgc tgaaagtgaa aaagcgcagc ctgaagcgga agtcgttgca
    21661 cagccggaac cggtcgtaga agaaacgccg gagccagtgg ctatcgaacg tgaagagctg
    21721 ccgttgccgg aagacgtcaa cgccgaagag gtttcgccag aagagtggca ggctgaagcg
    21781 gaaaccgtag agattgtcga agcggcggaa gaagaagcgg ctaaagaaga aatcaccgac
    21841 gaagagctgg aagcacaggc gctggctgcc gaagcggcag aagaggcggt gatggtggtt
    21901 cctccggtag aagagcagcc ggtggaagaa atcgctcagg agcaggaaaa accgaccaaa
    21961 gaaggtttct tcgcgcgcct gaaacgcagc ctgttaaaaa ccaaagaaaa tctcggttcc
    22021 ggatttatca gcctgttccg cggtaaaaaa atcgacgatg atctgtttga ggagctggaa
    22081 gaacagctgt tgatcgccga tgtgggcgtg gaaaccacac gtaaaattat caccaatctg
    22141 acggaaggcg catcccgcaa gcagcttcgt gatgccgagg cgctctatgg cctgctgaaa
    22201 gaagagatgg gcgagattct ggcgaaagtc gatgagccgc tgaatgttga aggtaaaacg
    22261 ccgttcgtga tcctgatggt gggcgtcaac ggtgtgggta aaactacgac gattggtaag
    22321 ctggcacgtc agtttgagct gcagggtaaa tcggtgatgc tggcggcggg cgatactttc
    22381 cgtgcagcag cggttgaaca gcttcaggtc tggggtcagc gcaacaatat tccggtgatt
    22441 gcccagcata ccggtgcgga ttccgcctct gttatcttcg acgccattca ggcagctaaa
    22501 gcgcgtaata tcgacgtcct gattgccgat accgccggac gcctgcagaa caaatcgcac
    22561 ctgatggaag agttgaagaa aatcgtccgc gtgatgaaga aactcgacgt tgaagcgccg
    22621 catgaagtta tgctgactat tgatgccagt accgggcaga atgcggtaag ccaggccaaa
    22681 ctgttccacg aagccgttgg cttaaccggc atcacgctaa cgaaactgga cggcacggcg
    22741 aaaggcgggg taattttctc ggtggctgac cagtttggta tccctatccg ctacattggt
    22801 gtcggcgaac gtattgagga tttgcgtccg tttaaggcgg acgactttat agaggcactt
    22861 tttgcccgag aggattaaca atgattcgct ttgaacatgt cagcaaggct tatctcggtg
    22921 ggagacaggc gctgcagggc gttacgttcc atatgcagcc gggtgagatg gcgtttctga
    22981 ccggtcattc cggcgcaggg aaaagtaccc tcctgaagct gatctgtggg attgagcggc
    23041 ccagcgccgg gaaaatctgg tttagcggcc atgacatcac gcgtctgaaa aaccgtgaag
    23101 ttccgtttct gcgccgccag attggcatga ttttccagga tcaccatcta ctgatggacc
    23161 gtactgtcta cgataacgtg gcgatcccgc tgattatcgc tggtgccagc ggtgacgata
    23221 ttcgtcgccg ggtgtcggcg gcactggata aagtcgggct actggacaaa gcgaagaact
    23281 tccctattca gctttccggc ggtgaacaac agcgtgttgg cattgcccgc gcggtggtga
    23341 ataagcccgc ggtactgctg gcggacgaac cgaccggtaa cctggacgac gcgttgtcgg
    23401 aagggattct acgtctgttt gaagagttta accgcgttgg ggtaaccgta ttgatggcaa
    23461 cgcacgacat caacctgatt tcacggcgtt cctatcgcat gctcaccctg agcgatggtc
    23521 acttgcatgg aggcgtgggc catgaataag cgcgatgcaa tcaatcatat tcggcagttt
    23581 ggtgggcgtc tggatcgctt ccgtaaatcg gtcggcggct caggcgacgg cggtcgtaac
    23641 gcaccgaaac gcgcgaaatc ctcgccaaaa ccggtaaatc gcaaaaccaa cgttttcaac
    23701 gaacaggtgc gctatgcctt ccacggcgca ttgcaggatc tgaaaagcaa accgttcgcc
    23761 acgtttttaa cggtgatggt tatcgccatc tccctgacgc tgcccagcgt ctgttatatg
    23821 gtgtacaaaa acgttaatca ggcggcgacg cagtattatc cgtcaccgca aatcactgtt
    23881 tacttgcaaa aaacgctgga cgatgacgcc gctgcgggcg tggtggcaca gttgcaggcc
    23941 gagcaaggcg tggagaaagt gaactatctt tctcgtgaag acgcactggg cgagttccgt
    24001 aactggtctg gttttggtgg tgcgctggat atgctggaag aaaacccgct tccggcagtg
    24061 gcggtggtga tcccgaaact cgattttcag gggacggaat cactcaatac gctgcgtgat
    24121 cgtatcacgc agattaacgg cattgacgaa gtgcggatgg atgacagctg gtttgcccgt
    24181 ctggcggcgt tgaccgggct ggtcgggcgc gtttcggcga tgatcggcgt gttgatggtg
    24241 gcggcagtgt tcctcgtcat cggcaacagc gtacgtctga gcatctttgc tcgccgtgac
    24301 tccatcaacg tgcagaaact gattggtgcg acggatggtt tcatccttcg cccgttcctg
    24361 tatggtggcg cactgctggg attttctggc gcattgttgt cattaatttt gtcagaaatt
    24421 ctggtgctgc gattgtcatc ggcggttgcg gaagtggcac aggttttcgg aacgaatttt
    24481 gatatcaatg gtttatcgtt cgatgaatgc ctgctattgc tgctggtatg ctcgatgatt
    24541 ggctgggtgg cagcgtggct tgccacggta caacatttac gccactttac gcctgaataa
    24601 taaaagcgtg ttatactctt tccctgcaat gggttccgta gcagggaaag agatcccgtt
    24661 gtctcttccc ggtatttcat ctctatgtca cattttgtgc gtaatttatt cacaagcttg
    24721 cattgaactt gtggataaaa tcacggtctg ataaaacagt gaatgataac ctcgttgctc
    24781 ttaagctctg gcacagttgt tgctaccact gaagcgccag aagatatcga ttgagaggat
    24841 ttgaatgact gacaaaatgc aaagtttagc tttagcccca gttggcaacc tggattccta
    24901 catccgggca gctaacgcgt ggccgatgtt gtcggctgac gaggagcggg cgctggctga
    24961 aaagctgcat taccatggcg atctggaagc agctaaaacg ctgatcctgt ctcacctgcg
    25021 gtttgttgtt catattgctc gtaattatgc gggctatggc ctgccacagg cggatttgat
    25081 tcaggaaggt aacatcggcc tgatgaaagc tgtgcgccgt ttcaacccgg aagtgggtgt
    25141 gcgcctggtc tccttcgccg ttcactggat caaagcagag atccacgaat acgttctgcg
    25201 taactggcgt atcgtcaaag ttgcgaccac caaagcgcag cgcaaactgt tcttcaacct
    25261 gcgtaaaact aagcagcgtc tgggctggtt taaccaggat gaagtcgaaa tggtggcccg
    25321 tgaactgggc gtaaccagca aagacgttcg tgagatggaa tcacgtatgg cggcacagga
    25381 catgaccttt gatctgtctt ccgacgacga ttccgacagc cagccgatgg caccggtgct
    25441 ctatctgcag gataaatcat ctaactttgc cgacggcatc gaagatgata actgggaaga
    25501 gcaggcggca aaccgtctga ccgacgcgat gcaaggtctg gacgaacgca gccaggacat
    25561 catccgcgcg cgctggctgg acgaagacaa caagtccacg ttgcaggaac tggctgaccg
    25621 ttacggtgtt tccgctgaac gtgtacgcca gctggaaaag aacgcgatga aaaaactgcg
    25681 cgctgctata gaagcgtaat ttccgctatt aagcagagaa ccctggatga gagtccgggg
    25741 tttttgtttt ttgggcctct acaataatca attccccctc cggcaaaacg tcaatcccca
    25801 cgcagattgt taataaactg tcaaaatagc tattccaata tcataaaaat ggggtatgtt
    25861 ttagcagagt atgctgctaa agcacgggta gtcatgcata aaacgaaata aagtgctaaa
    25921 tcacaacacc acaacacacg taataaccag aagaatgggg attctcagga tgaatataaa
    25981 gggtaaagcg ttactggcag gatgtatcgc gctggcattc agcaatatgg ctctggcaga
    26041 agatattaaa gtcgcggtcg tgggcgcaat gtccggtccg gttgcgcagt acggtgacca
    26101 ggagtttacc ggcgcagagc aggcggttgc ggatatcaac gctaaaggcg gcattaaagg
    26161 caacaaactg caaatcgtaa aatatgacga tgcctgtgac ccgaaacagg cggttgcggt
    26221 ggcgaacaaa gtcgttaacg acggcattaa atatgtgatt ggtcacctct gttcttcatc
    26281 aacgcagcct gcgtctgata tctacgaaga cgaaggcatt ttaatgatca ccccagcggc
    26341 aaccgcgccg gagctgaccg cccgtggcta tcagctgatc ctgcgcacca ccggcctgga
    26401 ctccgaccag gggccgacgg cggcgaaata tattcttgag aaagtgaaac cgcagcgtat
    26461 tgctatcgtt cacgacaaac agcaatacgg cgaaggtctg gcgcgagcgg tgcaggacgg
    26521 cctgaagaaa ggcaatgcta acgtggtgtt ctttgatggt atcaccgccg gggaaaaaga
    26581 tttctcaacg ctggtggcgc gtctgaaaaa agagaatatc gacttcgttt actacggcgg
    26641 ttatcacccg gaaatggggc aaatcctgcg tcaggcacgc gcggcagggc tgaaaactca
    26701 gtttatgggg ccggaaggtg tggctaacgt ttcgctgtct aacattgcgg gcgaatcagc
    26761 ggaagggctg ctggtgacca agccgaagaa ctacgatcag gttccggcga acaaacccat
    26821 tgttgacgcc atcaaagcga aaaaacagga cccaagcggc gcattcgttt ggaccaccta
    26881 cgccgcgctg caatctttgc aggcgggcct gaatcagtct gacgatccgg ctgagatcgc
    26941 caaatacctg aaagcgaact ccgtggatac cgtgatgggt ccgctgacgt gggatgagaa
    27001 aggtgatctg aaaggctttg agttcggcgt atttgactgg cacgccaacg gcacggcgac
    27061 cgatgcgaag taatcattaa tcggcaactt tgggttgccg ccaaattgct aatatcgagt
    27121 acgttgcttc atgccggatg cggcgtaaac gccttatccg gcctacaaga tccaaagaaa
    27181 tcagtaaatt gcaacacaca ttgtaggcct gataagcgta gcgcatcagg caatttctcg
    27241 ttggtcatca cttttgaaat caacttgacg attaacgctt ctcccagccg ccctgttgcg
    27301 ccgtaaatcc cagtgcctgc ataaacgccg tcatcacacc gcgatcttcc acgcctgcat
    27361 ccgccatcca ccagcatgaa acgccaggat tgttacgcaa aacctcttcc agcagatatt
    27421 gccccacacc gcgacgtcgg gtgacttccc gcacacgcag ggaatccagt gctccctcgg
    27481 tgccgcttaa ggttacccgt acggcagcga gcaggcgctc gttaaaacgc gcggcgtaga
    27541 tacggtggtt atcgtcaacc tgtaaagagg aaggggaata ctccggccag atcttttgca
    27601 ggtcaatccg gtcctggtcg ctaaagtttt ctaatcgaat gatggtcagc ttcatgggta
    27661 acccgtgtaa atcacaaaag tggaaccagt gtagcgaaat aatttaatcg gaggctttct
    27721 cttttttatt tcttttggca ggtgattaat tttttaacag caataattac aaagttaaaa
    27781 cattagagaa tgaaaaatac ccagcataat cccctgaatg gtagtgaatt attccgccct
    27841 ttgtgccgtt attttatgct ga
//
LOCUS       DAJTSL010000040        27206 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTSL010000040 DAJTSL010000000
VERSION     DAJTSL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27206
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.040"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(40..1338)
                     /gene="eno"
                     /locus_tag="OO433_001537"
     CDS             complement(40..1338)
                     /gene="eno"
                     /locus_tag="OO433_001537"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6050057.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1426..3063)
                     /gene="pyrG"
                     /locus_tag="OO433_001538"
     CDS             complement(1426..3063)
                     /gene="pyrG"
                     /locus_tag="OO433_001538"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6050058.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3291..4082)
                     /gene="mazG"
                     /locus_tag="OO433_001539"
     CDS             complement(3291..4082)
                     /gene="mazG"
                     /locus_tag="OO433_001539"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6050059.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEESYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4153..4488)
                     /gene="mazF"
                     /locus_tag="OO433_001540"
     CDS             complement(4153..4488)
                     /gene="mazF"
                     /locus_tag="OO433_001540"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR6050060.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4488..4736)
                     /gene="mazE"
                     /locus_tag="OO433_001541"
     CDS             complement(4488..4736)
                     /gene="mazE"
                     /locus_tag="OO433_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR6050061.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4814..7048)
                     /locus_tag="OO433_001542"
     CDS             complement(4814..7048)
                     /locus_tag="OO433_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6050062.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7096..8397)
                     /gene="rlmD"
                     /locus_tag="OO433_001543"
     CDS             complement(7096..8397)
                     /gene="rlmD"
                     /locus_tag="OO433_001543"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6050063.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     SALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8454..11210
                     /gene="barA"
                     /locus_tag="OO433_001544"
     CDS             8454..11210
                     /gene="barA"
                     /locus_tag="OO433_001544"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6050064.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11442..12782)
                     /gene="gudD"
                     /locus_tag="OO433_001545"
     CDS             complement(11442..12782)
                     /gene="gudD"
                     /locus_tag="OO433_001545"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6050065.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIMIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLCARDNAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12803..14143)
                     /locus_tag="OO433_001546"
     CDS             complement(12803..14143)
                     /locus_tag="OO433_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6050066.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELHVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14145..15515)
                     /gene="gudP"
                     /locus_tag="OO433_001547"
     CDS             complement(14145..15515)
                     /gene="gudP"
                     /locus_tag="OO433_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6050067.1"
                     /translation="MFRSATTSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRAT
                     LSIAGSEMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWS
                     MFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSI
                     FNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQ
                     KELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYF
                     FITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKT
                     PIVMGMLLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSG
                     GLFNMFGNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIEL
                     KPVAGQ"
     gene            complement(15932..16381)
                     /locus_tag="OO433_001548"
     CDS             complement(15932..16381)
                     /locus_tag="OO433_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6050068.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16399..17181)
                     /gene="truC"
                     /locus_tag="OO433_001549"
     CDS             complement(16399..17181)
                     /gene="truC"
                     /locus_tag="OO433_001549"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6050069.1"
                     /translation="MLEILYQDEWLVSVNKPSGWLVHRSWLDRDEKVVVMQTVRNQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17181..17510)
                     /locus_tag="OO433_001550"
     CDS             complement(17181..17510)
                     /locus_tag="OO433_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6050070.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQHALILAELEKLDA
                     LFADDAS"
     gene            complement(18132..18677)
                     /gene="syd"
                     /locus_tag="OO433_001551"
     CDS             complement(18132..18677)
                     /gene="syd"
                     /locus_tag="OO433_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6050071.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18745..19593
                     /gene="queF"
                     /locus_tag="OO433_001552"
     CDS             18745..19593
                     /gene="queF"
                     /locus_tag="OO433_001552"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6050072.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPKVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19705..21069
                     /gene="ppnN"
                     /locus_tag="OO433_001553"
                     /gene_synonym="ygdH"
     CDS             19705..21069
                     /gene="ppnN"
                     /locus_tag="OO433_001553"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6050073.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKAVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21626..22915
                     /gene="sdaC"
                     /locus_tag="OO433_001554"
     CDS             21626..22915
                     /gene="sdaC"
                     /locus_tag="OO433_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6050074.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22973..24340
                     /gene="sdaB"
                     /locus_tag="OO433_001555"
     CDS             22973..24340
                     /gene="sdaB"
                     /locus_tag="OO433_001555"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6050075.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24452..25207
                     /gene="xni"
                     /locus_tag="OO433_001556"
     CDS             24452..25207
                     /gene="xni"
                     /locus_tag="OO433_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6050076.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25312..26449)
                     /gene="fucO"
                     /locus_tag="OO433_001557"
                     /pseudo
     CDS             complement(25312..26449)
                     /gene="fucO"
                     /locus_tag="OO433_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
     gene            complement(26477..27124)
                     /gene="fucA"
                     /locus_tag="OO433_001558"
     CDS             complement(26477..27124)
                     /gene="fucA"
                     /locus_tag="OO433_001558"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6050077.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIAFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6578 a         7298 c         6554 g         6776 t
ORIGIN      
        1 tcccggaggc tggcattttt aaatcagata aagtcagtct tatgcctggc ctttgatctc
       61 tttacgaccg ttgtacggtg ctttttcgcc cagagcttct tcgatacgaa tcagctggtt
      121 gtatttagca acacggtcag aacggctcat agaaccagtt ttgatctggc ctgcagcagt
      181 accaacagcc aggtcagcga tggtagcgtc ttcagtttcg ccagaacggt gagagataac
      241 tgcagtgtag ccagcatctt tcgccatctt gattgcagcc agagtttcgg tcagagaacc
      301 gatctggttg aatttgatca ggatggagtt agcgatacct ttttcgatac cttctttcag
      361 gatcttggtg ttggttacga acaggtcgtc accaaccagc tggattttgt cgcccagaac
      421 tttggtctgg tatgcgaaac cgtcccagtc agattcgtcc agaccgtctt cgatagaaac
      481 gatcgggtac tgtttggtca gttcttccag gaagtgagtg aattcttcag aggtgaacgc
      541 tttgttgcct tcgccagcca gaacgtattt accatctttg tagaattcag aagctgcgca
      601 gtccatcgcc agagtgatgt ctttgcccag ttcataacca gcagctttaa cagcttcagc
      661 gataacagcc agagcttcag cgttggaacc caggttcggc gcatagccac cttcgtcacc
      721 aacagcagtg ttcatgcctt tcgctttcag aacttttgcc aggtgatgga aaacttcaga
      781 acccatgcgg atggcttctt tcacagtttt cgcgccaacc ggctgaatca tgaattcctg
      841 gatatcaacg ttgttgtcag cgtgctcacc accgttgatg atgttcatca tcggaaccgg
      901 catagagtat ttgcccggag taccgttcag ttcagcgatg tgctcgtaca gcggcatacc
      961 tttagcagct gcagcagctt tggcgttagc cagagataca gccaggattg cgttcgcgcc
     1021 gaatttggat ttgttttcgg tgccgtccag gtcgatcatg atcttgtcaa tgccagcctg
     1081 atctttagca tctttgccaa tcagcgcctg agcgatcggg ccgtttaccg cagcaacagc
     1141 tttggttacg cctttaccca ggaaacggga tttgtcgcca tcgcgcagtt ccagagcttc
     1201 acgggaacca gtagaagcac ctgacggagc agctgccata ccgacgaaac caccctccag
     1261 atgtacttcg gcttcaacag tcgggttacc acgggagtcg atgatttcac gaccgatgat
     1321 ttttacgatt ttggacatta ggttttcctc aagtcactag ttaaactgaa actccagaca
     1381 aacaacacgt acccagggta cgcgttgccg ttctaacttt tttacttact tcgcctgacg
     1441 tttctggaac tcgctggcgg ctttcacaaa gcctgcaaac agcgggtgac catcacgtgg
     1501 agtagaagta aactccggat ggaactggca agccacgaac cacgggtgat tcggaacttc
     1561 gatgatctcg accaactgat catccccgga acggcccgca acgcgcagac ctgcatcttc
     1621 aatctgtttc aacagcatgt tgttgacttc gtaacggtga cgatgacgct caacaattgt
     1681 cggcgcattg tacagctggc gaaccaggct atcgtcaacc aactggcact gctgtgcgcc
     1741 gagacgcatg gtaccgccga gatcgctctt ctcgctacga acttcaacgt tgccgttttc
     1801 atcgcgccac tcggtaatca gcgccacaac cgggtactta cagtctggca caaattccgt
     1861 agagttggcg ttctccatgt tggcaacatg gcgagcgtaa tcaattaacg ccacctgcat
     1921 acccaggcaa atgcccagat aaggaatatt gttctcacgc gcaaaacgcg cggtcgtaat
     1981 catgccttct acgccacgat agccgaaacc gccaggtacg aggattgcgt ccagaccttt
     2041 aaggatttca acgccgcgcg tttcaacatc ttgtgaatcg atcagtttga tgttgacgct
     2101 gacacgattc ttcagcccac cgtgtttcag tgcttcgatc actgatttat aagcatccgg
     2161 cagttcaatg tacttgccga ccataccgat ggtgacttca cttaccgggt tcgcttcttc
     2221 gaagataacc tgttcccatt cggacagatt cgcttccggg cagtttaagc tgaatcgttt
     2281 acaaatataa tcgtccagcc cctgagattt caacaggccc ggaattttat agatggaatc
     2341 gacgtctttc agagaaataa ccgctttttc cggaacatta cagaacaatg caatcttcgc
     2401 acgttcgttc gccggaacag cgcgatctga acgacaaatc aggatgtcag gctggatacc
     2461 gatggagagc agctctttta cagagtgctg agtcggtttg gttttgactt caccagacgc
     2521 tgccatgtac ggcaccagcg tcaggtgcat aaacagagtg tgctcacggc caatttcaac
     2581 agccatctgg cgaatcgctt cgaggaacgg caaggattcg atatcaccta ctgtaccgcc
     2641 gatttctacc agtactacgt catgaccttc gccaccttcc agcacgcgct ctttgattgc
     2701 gttagtgatg tgcggaataa cctgcacggt tgcgccgagg tagtcaccgc ggcgttcttt
     2761 acgcagaacg tcagagtaga tacgacccgt ggtgaagttg ttgcggcggc tcattttggt
     2821 acgaatgaaa cgctcgtagt gccccaggtc caggtcggtt tcagcgccgt cttcagtaac
     2881 gaacacttcc ccgtgttgga ttgggctcat agtacctgga tcgacgttga tgtacggatc
     2941 cagtttcatg atggtcacat tgaggccacg ggcttcaaga atggctgcga gggaggctgc
     3001 ggcaatgcct ttacccagag aggatacgac cccgccggtc acaaaaatat agttcgttgt
     3061 catgctgaac ctgagaagtt aggttgaaaa gacgatggaa taaccaggac gggaaagcag
     3121 tatacccgaa catgacctgt gccacaaact ttcattatcc ctcctcttcg ccagcgcact
     3181 attgaaatca ggagtgagaa aatagcccct ttggggtaaa tgtttttgac gcaaatcaag
     3241 cgcttgtcat ttaaaaaatg acacaaatgg cacttgaccg cgtaattccc ttagagatca
     3301 atttcctgcc gttttacctg ttgccagact tcttccattg tttcgaggtc aacacctgtc
     3361 atttccagtc cacgcgcggc aacaatacgc tccacttcgc gaaaacgacg ctcgaatttt
     3421 tcgttcgctt tttgcaatgc gatttctgct ttcgtcccta aatggcgagc cagattaacc
     3481 gtggcaaaca gcaggtcccc catttcctcc tccagtttag cctggtcgac aacagcctgc
     3541 cgcgcttcgt acatcacctc gtcgatctct tcgtagactt tatcaaccac cgggccaagc
     3601 gtcgtccagt cgaagccaac gttggcgcaa cgtttctgga ttttttgcgc ccgcattaaa
     3661 gccggtaaac tacgaggaat atcgtccagc gccgaatgct gcgctttctg cgcgcgctct
     3721 tcggttttga tttgctccca acgggcaagc acttcactac tgttttcggc agaactatca
     3781 gcaaaaacat gcggatggcg acgctctaat ttatcgctaa tagcagcgca aatatcatta
     3841 aagtcaaagc gcccttcttc ctgagccatt tgcgcgtaaa acaccacctg gaatagcaga
     3901 tcgcccagtt caccgcgaag atcgtcaaaa tcttcacggg cgatggcgtc cagcacttcg
     3961 tagctttctt caagggtgta aggcgcaatg gtggcaaatg tctgctcttt atcccacggg
     4021 cagccgtttt ccggatcgcg caggcgctgc ataatagtga gcaaacgatc gatttgattc
     4081 attgaattgt cctgaaaatt gcgggtctgt caggtggaaa cctgtgacca gaatagaagt
     4141 gagttagtaa cactacccaa tcagtacgtt aattttggct ttaatgagtt gtaattcctc
     4201 tggggcaact gttcctttct tcgttgctcc tcttgcccgc caggcgattc tttttacctg
     4261 atcagctaac gctacgccat cacgttcctg accggataaa acaacttcga acggatatcc
     4321 ttttgattgc gttgtacaag gaacacacag acacatacct gttttgttgt tgtacatgaa
     4381 aggactcagg acaacagctg gacgatgtcc agcttgctcg ctaccttttg tcgggtcaaa
     4441 atcaacccaa atcagatcgc ccatatcggg tacgtatcgg cttaccatta ccagacttcc
     4501 ttatctttcg gctctcccca gtcgatattc tcgtggaggt tttccggcgt gatgtcgttg
     4561 accagttcag caagcgtaaa tacgggctct ttacgcactg gctcaataat taatttgcca
     4621 tccaccaggt caatcttcac ttcatcatca atattcagat tgagcgcctg cattaacgta
     4681 gccgggatcc gcaccgccgg tgaatttccc caacgcttta cgctactgtg gatcatcacc
     4741 ctttcctcaa accgctatca tatgtagata cagtatatat caatctacat tgtagatacg
     4801 agcaaatttc tgcctaactc ccgtgcaacc gacgcgcgtc gataacatcc ggcacctggt
     4861 tgagtttacc cagcacgcgg cccagcacct gcaggttgta aatctcaatg gtcatgtcga
     4921 tggtcgccag ttgctgtttg gtgtcgctac ggctggcaac gccaagtacg ttcaccttct
     4981 cgttggcgag aatggtcgtg atatcacgta acaacccact acgatcatta gccaccacgc
     5041 ggaccaccag cgaatatccg gcggagtagc tctcacccca taccgcgtca acaatgcgtt
     5101 ctggcgcatg agagcgcagt tccgccagtt gttcgcaatc ggcgcggtgt actgaaatac
     5161 cgcgcccctg ggtaatgaag ccgacaatct catctccagg aatcggctgg cagcagcgcg
     5221 cgatgtggtg catcaggttg ccaacacctt cgactaccac gcgaccgtta tctttactgc
     5281 ggttttgcgg cgtgtagctt ttttgctgaa gttgcttcag cgcggcggcg tcctgctctt
     5341 cggcactcgg cttattaaat tgcgattgca ggaagttcac catctgattg agacggatat
     5401 ccccgccacc aatcgccgcc agcaactcgt cgacatcatt aaagttgtaa cgcggcagca
     5461 gatgtttttc tgcttctttc aggctgatcc ccagatgttc cagctcgtcg tcaaggattt
     5521 gccgcccagc cagaatgttt ttgtcacggt cctgtttacg gaaccaggcg tgaattttcg
     5581 aacgcccacg gctggttgtg acgtaaccga ggtttgggtt taaccagtca cggctggggt
     5641 tcggctgttt ctgggtgata atttcaatct ggtcgcccat ctgcagctgg taggtgaacg
     5701 gcacaatgcg cccgccaatt tttgccccga tgcagcggtg tccgacatca ctgtggatgt
     5761 ggtaagcgaa gtccagcggc gttgatcccg caggcaaatc aacgacatca cctttcggcg
     5821 taaagacata cacccggtcg tcaaagacct ggctgcgtac ttcgtcgagc atttcgccgg
     5881 aatcagccat ctcttcctgc cacgcaatca gtttacgcag ccaggcaatc cggtcttcat
     5941 gtcccgaacg tgcgccgcca gctgccgcgc cctctttata tttccagtgc gcagcaacac
     6001 ccaactctgc atcttcatgc atctgtttgg tgcggatttg gatctcaacg gttttgccac
     6061 ctggccccag aaccacggta tgaatagact gataaccgtt tggtttcggg ttagcgacat
     6121 aatcgtcaaa ctcatccggc aggtggcgat agtgagtgtg cactatcccc agtgcggcat
     6181 aacaatcctg taaacgctcg gcgacaatac gtaccgcacg cacatcaaac agctcatcga
     6241 aggcgaggtt ctttttctgc attttgcgcc agatgctgta gatgtgtttc ggacggccat
     6301 acacttcagc tttaacgcct tcagctttca tctcagcgcg cagatgaccg acaaactctt
     6361 cgatatagtg ttcgcggtcg agacggcgtt catgcagcag ttttgcgatg cgtttgtatt
     6421 cggtcgggtg cagataacgg aagcagtaat cttccagctc ccatttcagt tgcccaatcc
     6481 caagacggtt cgccaacggc gcgtagatat tggtgcactc ttttgccgcc agtacgcgtt
     6541 catcttccgg cgcatctttt acttcgcgca gatgagcaat acgctccgcc agtttgatga
     6601 ccacgcagcg aaaatcatcg accatcgcca ataacatccg gcgaacgtta tcgacctgtt
     6661 cggaggaaac agaatcagtg tgcgtcgctt tcagctggcg gatcgccgcc atatcacgca
     6721 cgccgtgaat aaggttaacg acagacttac cgacgctctc acgcagcaca tcttcgctga
     6781 ctacgttggc atcggccaga gggaacagca gcgccgcccg cagcgtgtca atgtccatac
     6841 ttaatgtcga gaggatctcc accatctcaa caccacgcca caataacaga ctggcatccg
     6901 gatgcccctg cgtctgctgc agacaatacg cccaggtttc ggctaagcac tcacacgact
     6961 tctggctggt aatacccaga cttgcgatcc atttttccgg atcaaattca ccagccttat
     7021 tgatatgtgc acttcttacc gcaaccattg tcctctcctt tagggaccag acctgccgaa
     7081 atcggcaaat cgtaactatt taacgcgcga gaaaagtacc atcgattcca gatgtcccgt
     7141 gtgtgggaac atatccagca tcgccagtcg cgcaatggta tatcctgctt ttaataacgc
     7201 ttcgctatcc cgagccagcg ttgcagggtt acaggataca taaactatac gaataggttc
     7261 cagttttata atttgctgca taacacctgc ggcacctgct cgcgccgggt ccagcaacac
     7321 tttatcgaag ccgtttttcg cccacggctg ctttgtgaca tcttcttcaa gattttcgtg
     7381 ataaaacgtc acattctgta agccattaag acgcgcattc tgctggcctt tttccaccag
     7441 cgccggaaca ccttctacac cgaccacact ggcagcttgt gtcgccaatg gcagtgtaaa
     7501 gttgcccata ccgcagaaca gatccagtac gcgatcttca ggttgcacat ccagccattc
     7561 caacgcactc gctaccattt tttggttcac acccgcattg acctgaataa aatcgcgcgg
     7621 gctaaaagtt aagcgcaacc cgttagagtc ataccagggc atctcaccag agacggtttc
     7681 gagtatctca ctatcggggg cgagatacag atccaggcct tcagaatgcg aaaagcgttc
     7741 cagtttttcg cgatctgccg aacttagcgg tgcggtatgg cgcaaaatca tcagcgtgcc
     7801 gctggttgcc tgtaccagtt caacatgacc aagatggcgc attgcttgta agctgcccag
     7861 gcatgccctg actttgggca gcaatgcttc aagttggggc actaaaatgg gacattgttt
     7921 aacgtcgaca atgtcactgg agcccgcttt gcgaaacccc atctgaagtt gctgtgtttt
     7981 cggtaagtag ttcagactta aacgcgcgcg acggcgatag ccccagggaa catcggcgat
     8041 cacttcagag acttcgtgtt tcattaatcg ggcgagtgcc gcacttttgc ttcgctgctg
     8101 taaatccacg ctggcgtgtt gttgctgaca gccgccgcat acgccaaaat gaggacagcg
     8161 tggcgtttcg cgttccgggc tatcgcttaa ccggcgtacg actttagcgc gggcatactg
     8221 ttttttatct tcagtaacag taacttccgc gttttcctgc ggcaataatc cggggataaa
     8281 tagcgttttg ccgttatgtc gcgccacgcc ctgaccaaaa gagtcgaggt cgttgactga
     8341 aacggttatg atctgacgcg tcgtcgtgcg tcgttttgca gagtagaatt gcgccattgg
     8401 cgagactttc tcaatttaac agtgtgacct taattgtccc ataacggaac tccatgacca
     8461 actacagcct gcgcgcacgc atgatgattc tgatcctggc accgaccgtc cttattggtt
     8521 tattgctgag tatatttttc gtcgtgcatc gctataacga cttgcagcgt caactggaag
     8581 atgcaggtgc cagcattatt gagccgcttg cagtttctac tgaatatggc atgagcctgc
     8641 aaaatcgcga atctatcggt cagttaataa gcgtactgca tcgtcgccat tccgatattg
     8701 ttcgcgcgat ttcggtttat gatgaaaata accgactctt tgtcacctcc aattttcatc
     8761 ttgatccctc atcaatgcag ctcggcagca acgtgccgtt tcctcgccag ctcactgtca
     8821 ctcgtgacgg cgatattatg atcctccgca cgccgattat ttctgagagt tactcccccg
     8881 acgaatcgcc cagtagcgat gccaaaaata gtcaaaatat gttgggatat attgcgcttg
     8941 agctggatct taaatcggtt cgcttgcagc aatataaaga gatctttatt tccagcgtga
     9001 tgatgctgtt ttgtatcggt attgcgctta tttttggctg gcgcttaatg cgcgatgtaa
     9061 ccggtccgat tcgcaacatg gtgaataccg tcgaccgcat ccgtcgcggg caactcgaca
     9121 gccgggtgga aggatttatg ctcggcgagc tggatatgct gaaaaacggt atcaactcga
     9181 tggcaatgtc gctggctgct tatcacgaag agatgcagca caatatcgac caggcgacgt
     9241 ccgatctgcg tgaaacgctg gagcagatgg aaattcagaa cgttgagttg gatctggcga
     9301 aaaagcgcgc ccaggaagcg gcgcgtatta aatccgagtt tctggcaaat atgtcacacg
     9361 agctgcgtac accactgaat ggtgttattg gctttacccg cctgacgctg aaaacagaat
     9421 taacaccaac gcagcgcgat cacctgaata cgattgaacg ttcggcaaat aatttgctgg
     9481 caattattaa tgatgttctc gacttctcga aactggaagc aggtaagctg attctggaaa
     9541 gtattccatt cccactacgc agcacgctgg atgaagtcgt tactctgctg gcacattctt
     9601 ctcacgataa agggttggaa ctgacgctca atattaaaag cgacgtgcct gataacgtga
     9661 tcggcgaccc actgcgatta cagcaaatca tcactaacct ggtggggaat gcaattaaat
     9721 tcaccgagaa tggcaacatt gatattctgg tagaaaaacg tgcgctgagt aataccaaag
     9781 tgcagattga agtgcagatt cgggataccg gcattggtat tcctgaacgc gatcaatcgc
     9841 gcttattcca ggccttccga caggctgatg ccagtatttc ccgccgtcat ggtggcaccg
     9901 ggctggggct ggtgattaca caaaaactgg ttaatgaaat gggcggcgat atttcgttcc
     9961 atagccagcc gaatcgcggt tcaactttct ggttccacat taatctcgat ctgaacccga
    10021 acattattat cgaagggcca tccacccagt gcctcgcagg taagcgcctg gcctatgtcg
    10081 aaccaaactc cgcagcagcg caatgcacgc tggatatttt aagtgaaacg ccgctggaag
    10141 tggtttatag cccaacgttc tccgcgctgc ctcccgcgca ttacgacatg atgttgttag
    10201 gcatcgcggt gaccttccgc gagccgctaa caatgcaaca tgagcgatta gcgaaagcgg
    10261 tatcgatgac cgatttcctg atgctggcac ttccttgcca tgcacaagtc aatgctgaaa
    10321 aactcaagca agatggtatc ggcgcgtgtc tgctgaaacc attaacacct acacgcctgt
    10381 tgcctgccct gacggaattt tgtcatcaca aacaaaacac gcttttgcct gtaaccgatg
    10441 aaagtaagct ggcaatgaca gtcatggcgg ttgatgacaa ccccgctaac ctgaaactta
    10501 tcggcgcatt gctggaagat atggtgcaac atgtggaact ttgcgatagc gggcatcagg
    10561 cggttgaacg ggcgaaacag atgccgttcg atttgatctt aatggatatt caaatgccgg
    10621 acatggatgg cattcgggcc tgcgaactca tccaccagct cccccatcag caacaaacgc
    10681 cggttatcgc ggtaacggcg catgcaatgg ccgggcaaaa agagaagctg cttggcgcag
    10741 ggatgagcga ttatctggcg aaaccgattg aagaagagcg attgcataat ttgttgttgc
    10801 gctacaagcc tggcagcggt atttcctctc gcgtcgtgac gcccgaagtc aacgaaattg
    10861 tggtgaaccc gaatgcgacc ctcgactggc aactggcact acgccaggca gcaggaaaaa
    10921 ccgatttagc gcgcgatatg ctgcaaatgt tactcgattt cctgcctgaa gttcgcaaca
    10981 aagttgagga acagctggtt ggagaaaacc cggaaggcct ggttgatttg attcataaac
    11041 tgcatggcag ttgcggctat agcggtgtgc cgcgtatgaa gaatctctgc caactgatcg
    11101 aacaacagct acgtagcggt actaaagaag aagatttgga gccggagctg ctggaactgt
    11161 tggacgagat ggataatgtc gcgcgcgaag ccagcaaaat tctcgggtaa cggacgtctt
    11221 gtcgagtagc aatcggcttt ttatgacgct ggtttcagat tggtgacaaa gtgcctgtgt
    11281 ttatgccgga tgcggcgtta acgccttatc cggcctacaa aaccaattaa attcaatgaa
    11341 ttaaaaaatg atgtagacct gatacatagc acaacaggct attttgcgtt tagcatcagt
    11401 ctcaagccgg ctccagatag agccggtttt ggttttctgt cttaacgcac catgcacggg
    11461 cgcttgttat cgaacgtcca gccaggaatc agatactgca ttcccatcgc attgtcacgc
    11521 gcgcaaagcc cgtgtttctg atacagctca tgggctttca tcacttgatc catatcgatt
    11581 tctacaccca gccccggttt ttccggcacc tgaaccagac cgcctttgat ctcaaacggt
    11641 tctttggtca agcgctgatt gccttcctgc caaatccagt gcgtatcaat cgcggtgatt
    11701 ttacccggtg cagcggcggc aacatgggta aacatcgcca gggaaatatc gaagtggttg
    11761 tttgagtgtg aaccccaggt caggccaaat tcatggcaca tttgcgccac acgtaccgaa
    11821 ccttgcatcg tccagaaatg cggatccgcc agcgggatat caacggattg cagggagagc
    11881 gtatggccca tctgccgcca gtcggtggcg atcatattgg ttgcggtcgg caggccagtt
    11941 gcgcgacgga actctgccat cacttcacgc ccggagaaac cttgctccgc accacacgga
    12001 tcttctgcat aagccagcga ccctttcagg tatttaccaa ttttaatcgc ttcgttcagc
    12061 gaccaggcac cgttaggatc gagcgtaata cgcgcctgcg ggaagcgttg cgccagtgcc
    12121 acaatagact cggcctcttc ttccccggcc agtacaccgc ctttcagttt gaaatcgttg
    12181 aagccatatt tttcatacgc cgcttccgcc aggcgcacca ccgcatccgg cgtcatcgct
    12241 tcttcatgac gcaggcgata ccagtcgcat gagtcatccg gctggctttg atacggcagc
    12301 ggtgtggctt tgcgatcacc gacgaagaac agataaccga gcatttcgac ttcgctacgc
    12361 tgttgaccat cgcccagcag ggatgccacg tttaccccca gatgctgccc cagcagatcc
    12421 agcattgccg cttctatccc ggtaactaca tgaatagtgg tacgcaaatc aaatgtctgc
    12481 aaaccgcgcc caccagcatc acgatcggca aaagtattac gcaccagcgt cagaacgttt
    12541 ttgtattcac ccagcgtttt acctaccacc agcggaatcg catcttccag cgttttacgg
    12601 attttctcgc cgccgggaat ttcccctacg ccagtgtgac cagaattatc tttgataatc
    12661 ataatattac gcgtaaagaa cggtgcgtgt gcaccactca gattcatcag catactgtca
    12721 tgacccgcca ccgggatgac ctgcatttca gtaacaacag gcgtcgtaaa ttgagaactc
    12781 atatttatgt cctttttcag aatcaatgac ggccgaaaac gggacgttta cggtcaaagg
    12841 tccagccggg gatcaggtac tgcatcggac ctgcgtcgtt acgcgcaccg ccaggcagac
    12901 gtttataggc ctcatgtgcc ttttgtacct gttcccagtc cagttccacg cccagaccgg
    12961 gcgcatcagg aacggcaatt tttccgtttt taatctccag cggatttttg gtcaggcgac
    13021 aatcgccctc ctgccaaatc cagtgggtat cgatagcggt aggattaccc ggtgccgccg
    13081 cgcccacatg ggtaaacatc gccagagaga tatcgaaatg gttattagaa tggcagcccc
    13141 aggtcagccc ccagtcgtcg caaagctgcg ccacacggac tgcaccggaa agcgtccaga
    13201 agtgcggatc ggcaagtgga atatctaccg cattgagcat caccgcatga cccatttcgc
    13261 gccagttggt ggcgatcatg ttagtcgcga cgggcaagcc ggtcgcccgc cgaaactccg
    13321 ccataacttc acgaccggag aaaccctgtt ctgcgccgca tggatcttcg gcataggtaa
    13381 gaacatcatt cagccctttg catagagaaa tggcttcatc aagtagccag gcaccgttgg
    13441 gatcaacggt aatccgcgca tcagggaagc gtttcttcaa tgcacgaaca gtgtcgattt
    13501 cttgctcgcc aggtaacacc ccgcccttaa gtttgaaatc tttaaagcca tagcgatcct
    13561 gtgaggcttc cgccagacgc acaacggctt cgctgttcat cgctttctga tggcgcaact
    13621 gataccactc atggttgccc ggcgtatttt ccagataagg aagatcggtt ttggtccgat
    13681 caccgatata aaacagataa ccgaggacgg taatagtctc gcgttgcttg cctggcccca
    13741 acagttcgca gaccggaaca ttcagcgcct tacctagcag gtcaagcaag gcggcttcca
    13801 gcgccgccac ggcattaacg tgcaattcaa aagtccaggc acctttgccg aaggtatcaa
    13861 aatcggctgc ctgattacct ttatgcacct gctgaaccac tttattcagg cgcgcaactt
    13921 cctggcccag aaccatcgga atagcatcga ccagtgtttg ataaatcacc tctccgcccg
    13981 gcgcttcacc aatgccggta tgcccggcgt tatcggtgag taccacaata ttgcgggtga
    14041 aatatgcgtt atgtgcccca ccaatattaa gcaacatgct atcgtgcccg gcgaccggaa
    14101 tgactttcat atcagtaata acaggactgg attgtgtcgt catcttattg ccccgcgaca
    14161 ggtttcagct cgatacgctt gatatcgccc accagcacca ggtagctcag taccgcgatt
    14221 aaggcatgaa caccaacata aatcagcgcc ccattaaagg agccagtcgt gccaacgata
    14281 taaccaattg cgattggcgt gacgatgcca gaaatgttac cgaacatgtt gaacaggcca
    14341 ccggaaagac cgctgatctc ttttggcgcg gtatctgcca ttactgccca acccagcgcc
    14401 ccgatgccct taccgaagaa ggccagcgcc ataaagccga tgatcatcca ctcaacgttg
    14461 acgtagttgc agaacaccat caccatcgac agcaacatgc ccattacgat cggtgtttta
    14521 cgcgcaatgt tcagcgatcc cgtgcggcgc atcagccagt cggaaataat cccacccagc
    14581 acaccgccga taaaaccgca aaccgccgga acggaagcca caaagcccgc tttcagaatt
    14641 gacatcccac gcgcctgcac cagataaacc ggaaaccagg taatgaaaaa gtaagtcagg
    14701 gcgttgatac agtactgacc gatataaacg ccgatcatca tccgtgaccc gagcagttgt
    14761 ttgatctgcc cccacttcac gctgaacggc actttaactt tggtgttttg ctgatccata
    14821 ttgatcagcg cgccacccgc ggcgatgtac tccagctctt tctgatttac ccccggatgt
    14881 tgatttggct cgtggatgac tttcaaccag ataaagctga tgacaatccc cagaccgccc
    14941 ataaagaaga agacgtgtga ccagcccact tcatgcgtca gccagcccat aatcggcgcg
    15001 aagatcaccg ttgcgaagta ttgagcggag ttaaaaatcg acaccgccgt tcccctttcc
    15061 tgcgccggaa accaggccgc aacaatgcga ctgttgccgg ggaaagatgg cgcttcagca
    15121 agcccgacca ggaagcgcag agtaaacagg gcaacgataa tgccgaatcc actaaagata
    15181 tcgacgaagc cttgcagcaa ggtaaacatc gaccagataa agatcgacca gaagtagacg
    15241 cgttttgaac caaaacgatc cagcaaccaa ccaccaggga tctgcccgat aacataagcc
    15301 catgagaagg cagagaacac atagcccatt cccacgggat caaggccgat atctttggcc
    15361 atttccgaac cggcaataga aagcgtagcg cggtcgccgt agttgaagga tgtgacgata
    15421 aacaacatca ccactatcca gtaacgagca ttcgtgcgtt tttccacact gctcgcagcc
    15481 tgacttaaag aactcgttgt tgcactcctg aaaattcgcg ttagccacgc taactctgga
    15541 ctgcgacata gccaggaaat cagaggtgac gtagggtgtt ttttgccgtt tttataggtc
    15601 gttcgccgaa tacggcgcgt gtttatatct ggcaatagca gtataaaaag cgcgccatag
    15661 cggctcaccg tgcaacaaca caacattaat gcgttcaatg aggcccgatt ttggcattag
    15721 ccctggacgg tggaatccac ttcacggaaa tgaaaacaag aacaagaaag gaagggttaa
    15781 aacgaagaaa taaagaagag tatgaaatgg atcgcttgac tccaggcaaa cgccagtaaa
    15841 aatccgcgct atgaagcagt ttttactggc atttgcctga aaagattcga ttcagcaccg
    15901 ctaaaacgac atttaccgct cgctgaacat atcaggacaa cagcgtgccc cactgttcga
    15961 cccacggatt tgattccgtt tccggttccg ggttctcact ggcgtcaatc aacagcattt
    16021 cgccaactcg ctgagcgctc tgttcctgca acaaggcatc gaactgtttg ccgccattgc
    16081 agaaatttac ataactgctg tcgccgagcg caatcacgcc ataacgcaga ttcggctgga
    16141 agcccagact atctttgatt ccctgaaaga gcggcacaat gctatcagga aggtcgccct
    16201 gcccggtcgt ggacgtaacc accagaacat acttatcctg ataaggcagc cagtcgctta
    16261 attcaggatc ttcaaatacc gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt
    16321 cttccgccac taacagcgaa tttccgtaca tggtgccgac aaaaatacca atttccgcca
    16381 taccgttttc tccctggatt aggaacttat ctctccatcc tgacccgatg gcgcactgaa
    16441 ctcaaccctt tcattttcag gaagcagacc gcgccagcca aattgtgata acgcctgcat
    16501 ccaggtgtcg tccaggcccg cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa
    16561 tgacaactga ctggcatgta gcattaaccg ctggaggcca aaatgctcag caccgctgcg
    16621 attctggcgt aaatcgccat gtttgctatc gccaataatc ggatgacgca aatgggcaag
    16681 atgtcggcga agctgatgtt tgcgtccggt tttcggctcc agttccacca ggccgtagcg
    16741 cgtggtcggg taacgtccgg tcgctaccgg catttctacg gtcgccagac cgcgataatg
    16801 cgtcactgct ggctgcgggc ctttatcttc gcgggcaaat ttatcagcga ttttgtccag
    16861 ttcttccacc agtggataat ccagcaccgc ttcttccatc aaccagccgc gcacaatcgc
    16921 atggtaacgt ttctggattt ggtgctgttc aaactgttgt gccagcagcc gtccggcctc
    16981 gctggataat cccatcaaca acacaccaga agtgggtcgg tccagacgat gagcagtaaa
    17041 aacatgctgt cctatctggt tacgcacggt ttgcatgacc actactttct cgtcgcgatc
    17101 cagccagcta cggtgaacca gccagccgga gggtttattt accgaaacca gccattcatc
    17161 ctgatagagt atttccagca ttagctcgca tcatccgcaa aaagagcatc cagtttttcc
    17221 aactcagcca gaataagcgc gtgttgcgga tggtccgtcg ccagcgccat ttcataatag
    17281 ggtgcaacag caaaagcgcc cggtaacggc tggttgttat ttaacaaatc gtgcattcgc
    17341 gggatcagca cccactgcaa ccactccagt ggttccatgg tgtccataaa gaacggttgg
    17401 gtactattaa attgatgcgg ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt
    17461 aacgcttcaa gcgcctgcaa ctggagacga acgcggtcat gagtggtcat aaagcaacct
    17521 caataagaaa aactgccgcg aaggatagca ggaataaaaa aagggagcac tgtattcaca
    17581 gcgctcccgg ttcgtttcgc agcattccag ctacttttgt tgctccctgc tcatccttga
    17641 caacttttcc tctggccttg cggccaatcg ttcatcctga actattgctt cctgctcaca
    17701 ccaccccgat gtgatacttc atcctgaagt gtccttggcc ttcctgacca gccgaatcat
    17761 cctgaccggt tctcattctc catcctggag gtgtccttta acgcgtcctg cgtcatcctc
    17821 ttcgcttcat ccagaagcct ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc
    17881 atcatcctga tgttcacttc gttgtctgac tccctgtcga cgaagataga atcgtctttt
    17941 tcgcgaagtc ttacaaggcg cttacagaca atgccttaag gaaaatttca tatgaaagtt
    18001 aagaattaac aatcaacatt ttgattttaa atgaaaataa cgattcttat ctttttaagt
    18061 attcgcttat ttcctggcga tctcctacag agtctgtaag agatctctca caaccagatg
    18121 gggtatttgg attacagaag aggcttaagt tgattaagga attccgcaag atttgaggct
    18181 aaatgcgtcc gtttacgtgt tcccagcgtc tctttgcaca cttcaccgga gagattacac
    18241 accgagatga cctctaactc ttcttccagc gtcgcgataa atagcgtggg cggcagtttt
    18301 agccttttct gagtcaccag atgaccaatc agattttcct gcacccgacg gaagtcgtct
    18361 tcactccagg tttgcagcaa tgtcagtttg atatcgccaa actgcgcgtg catatccccg
    18421 gcaaactgag tcgtataaaa ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt
    18481 tcaaccgcgt ttacattttg ttccccggta aacggctggg gttgccagta tacggcatct
    18541 tcggtagttg aaataatgca cggtgaagga acaccgtaca gttcctcact taacggccag
    18601 cttttatgct cttcatgcca cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt
    18661 gcggtcaaat cgtccactaa tttctctctt cacttaaacc agatacactt gtcgtttagt
    18721 ttatctggtt tatgacggtg aaacatgtct tcttatgcaa accatcaggc acttgcgggc
    18781 ctgactcttg gaaaatcaac cgattaccgg gatacctatg acgccagcct actgcaaggc
    18841 gttccacgca gcctgaatcg cgacccgctg ggtctgaaag cggataacct gccttttcac
    18901 ggtacggata tctggacgct gtatgaactt tcctggctga atgcgaaagg tttgccgaag
    18961 gtcgctgtcg gtcatgttga acttgattac accagcgtaa atctgattga gtcgaagagt
    19021 tttaagctct atctcaacag ttttaaccag acgcgtttta ataactggga tgaggtgcgc
    19081 cagacgctgg agcgcgactt aagcacttgc gctcagggta agattagcgt ggcgttatat
    19141 cgtcttgatg aactggaagg ccagccgata ggtcatttta atggcacttg cattgatgac
    19201 caggatatca ctatcgataa ctatgaattc actactgact atctggagaa tgccacctgt
    19261 ggtgaaaaag tagtggaaga gacgcttgtc agccacctgc tgaaatcaaa ctgcctgatc
    19321 acccatcaac cagattgggg ttcgatccaa attcagtatc gtggacgcca aattgacaga
    19381 gaaaaactgc tgcgttacct ggtctcattc cgtcatcaca acgagttcca cgaacagtgc
    19441 gtggaacgca tctttaatga cctgttacgc ttctgccagc cagaaaaatt gagcgtttac
    19501 gcacgttata cccgtcgtgg cggtctggac attaacccgt ggcgcagtaa tagcgatttt
    19561 gtcccatcga ccacaagact ggttcggcaa taaatttttt ctcaattttg cgtgctggat
    19621 tcacgcagaa ggttgtgaaa ggtcatcagg cagggctatt gtaatcaaag ggaatgacga
    19681 tattcgtccc ataaggagtt tttcttgatt acacatatta gcccgcttgg ctccatggat
    19741 atgttgtcgc agctggaagt ggatatgctt aaacgcaccg ccagcagcga cctctatcaa
    19801 ctgtttcgca actgttcact tgccgtactg aactccggta gtttgaccga taacagcaaa
    19861 gaattgctgt ctcgttttga aaatttcgat attaacgtct tgcgccgtga acgcggcgta
    19921 aagctggaac tgattaatcc cccggaagag gcttttgtcg atgggcgaat tattcgcgct
    19981 ttgcaggcca acttgttcgc ggtcctgcgt gacattctct tcgtttacgg gcaaatccat
    20041 aacaccgttc gttttcccaa cctgaatctc gacaactccg tccacatcac taacctggtc
    20101 ttttccatct tgcgtaacgc tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc
    20161 tgctggggcg gtcactcaat taacgaaaac gagtatttgt atgcccgtcg cgtcggaaac
    20221 cagctgggcc tgcgtgagct gaatatctgc accggctgtg gtccgggagc gatggaagcg
    20281 ccgatgaaag gtgctgcggt cggacacgcg cagcagcgtt acaaagacag tcgttttatt
    20341 ggtatgacag agccgtcgat tatcgccgct gaaccgccta acccgctggt caacgaattg
    20401 atcatcatgc cagatatcga aaaacgtctg gaagcgtttg tccgtatcgc tcacggtatc
    20461 attatcttcc ctggcggtgt gggtacggca gaagagttgc tgtatttgct gggaatttta
    20521 atgaacccgg ccaacaaaga tcaggtttta ccattgatcc tcaccggccc gaaagagagc
    20581 gccgactact tccgcgtact ggacgagttt gtcgtgcata cgctgggtga aaacgcgcgc
    20641 cgccattacc gcatcatcat tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg
    20701 atgccgctgg tgaaagaaaa tcgccgcgac acaggcgatg cctacagctt taactggtca
    20761 atgcgcattg cgccagattt gcaaatgccg tttgagccgt ctcacgagaa tatggctaat
    20821 ctgaagcttt acccggatca acccgttgaa gtgctagctg ccgacctgcg ccgtgcgttc
    20881 tccggtattg tggcgggtaa cgtaaaagca gtcggtattc gcgccattga agagtttggt
    20941 ccttacaaaa tcaacggcga taaagagatt atgcgtcgta tggacgacct gctacagggt
    21001 tttgttgccc agcatcgtat gaagttgcca ggctcagcct acatcccttg ctacgaaatc
    21061 tgcacgtaat ctcccctccc ccggtgacgt ttgttgccgg gttttctcgt ttttggtcac
    21121 ttactcatca actcatttca tttgttatat gaatgtttct taccaccctc acgcgacaaa
    21181 tatcatcaca gttaatatgt catacaattt atgttgcaac gcaaacgttt ctctattttc
    21241 ataaaccgta ttttatcgct atgaaaaaga aattatcgcc atgattaact aaaagtattg
    21301 attttttcag ttcaacctac atatattgcg cgccctggaa gaagtcagat gtcgtttaat
    21361 gggcaaatat tgcccttaaa ttctctttta cttttgattt acagagtaaa gcgttgggat
    21421 aatctatctt ccaagtagat tattgtattt gagatcaaga tcactgatag atacataact
    21481 tgtgtgtatc tttccgccct caaattatta cggcggtaaa tgattaagcc atcgccgata
    21541 gacagatttc atttttacgg tcaggcacct tcccgggctg aactggctaa aagctgaatt
    21601 atttgcattc ctccaggaga aatagatgga aacgactcaa accagcacga ttgcgtcgaa
    21661 agactctcgt agtgcctggc gcaagacaga caccatgtgg atgctgggcc tttacggcac
    21721 ggcaatcggc gcgggcgtgc tgttcctgcc aatcaacgcc ggtgttggcg gtatgatccc
    21781 gctgatcatc atggctatcc ttgcgttccc gatgacgttt tttgctcacc gcggcctgac
    21841 tcgcttcgta ctgtctggta aaaacccggg cgaagacatc accgaggttg tagaagaaca
    21901 ctttggtatt ggcgcaggta aactgattac cctgctctac ttcttcgcta tctacccgat
    21961 cctgctggtt tatagcgtgg caatcaccaa tacagttgaa agcttcatgt ctcaccagct
    22021 gggtatgacg ccaccgccgc gtgcgattct gtcgctgatc ctgatcgtgg gtatgatgac
    22081 catcgttcgc ttcggtgagc agatgatcgt taaagcgatg agtattctgg tgttcccgtt
    22141 tgttggcgta ttgatgctgc tggctctgta cctgatcccg cagtggaacg gcgcagcact
    22201 ggaaacgctg tctctggaca ctgcatctgc aaccggaaac ggtctgtgga tgaccctgtg
    22261 gctggcaatt ccggtaatgg tgttctcgtt caaccactct ccgatcatct cttctttcgc
    22321 cgttgcgaag cgtgaagagt acggcgatat ggcagaacag aaatgctcga agatcctggc
    22381 attcgcacac atcatgatgg tgctgaccgt aatgttcttc gtcttcagct gtgtactgag
    22441 cctgactccg gcagacctgg ctgcggctaa agagcagaac atctcgattc tgtcttacct
    22501 ggctaaccac tttaacgcac cggttatcgc gtggatggct ccgattatcg cgattatcgc
    22561 tatcaccaaa tccttcctcg gccactacct gggcgcacgt gaaggcttca acggtatggt
    22621 gattaaatct ctgcgtggta aaggtaagtc tatcgaaatc aacaagctga accgtatcac
    22681 tgcgctgttc atgctggtaa cgacctggat tgttgccacc ctgaacccga gcatcctggg
    22741 tatgattgaa accctgggcg gtccaatcat cgcgatgatc ctgttcctga tgccgatgta
    22801 cgcaattcag aaagtaccgg caatgcgtaa gtacagcggt cacatcagca acgtattcgt
    22861 tgtcgtgatg ggtctgattg caatctccgc aatcttctac tctctgttca gctaagtcct
    22921 ttcgcgccgc tttcgggcgg cgcttcctcc gttttaacgc gatgtatttc ctatgattag
    22981 cgtattcgat attttcaaaa tcggcattgg cccttccagt tctcataccg ttggaccaat
    23041 gaaagcgggt aaacaattta ccgacgatct gattgcccgt aacctgctta aagacgtgac
    23101 ccgcgtggtg gttgacgtgt acggctcgct ctctctgacc ggtaaaggcc accacactga
    23161 tatcgccatt attatgggcc tggcgggtaa cctgccggat accgtggata tcgattccat
    23221 ccctggtttt attcaggatg tgaatactca tggtcgcctg atgctggcaa acggtcagca
    23281 tgaagtggag ttcccggttg atcagtgcat gaacttccac gccgacaacc tttctctgca
    23341 tgaaaacggt atgcgcatta ccgcgctggc gggcgataaa gtcgtttaca gccagactta
    23401 ctactctatt ggcggtggct ttatcgttga tgaagagcat tttggccagc aggatagcgc
    23461 accggttgaa gttccttatc cgtacagttc agcagccgat ctgcaaaaac attgtcagga
    23521 aaccgggctg tcactctctg gcctgatgat gaaaaacgag ctagcgctgc acagcaaaga
    23581 agagctggaa cagcacctgg cgaacgtatg ggaagtcatg cgtggcggta ttgagcgcgg
    23641 tatttccacc gaaggcgtgt tgcctggcaa actgcgcgtt ccacgccgtg ctgcggcact
    23701 acgccggatg ctggtcagcc aggataaaac caccactgac ccgatggcgg ttgttgactg
    23761 gatcaacatg tttgcactgg cagtgaacga agagaacgct gctggcggac gcgtggtgac
    23821 tgcgccgact aacggtgcgt gcgggattat cccggcagtg ctggcgtact acgacaagtt
    23881 tatccgcgaa gtgaacgcta actcactggc tcgttacctg ctggtagcca gtgccattgg
    23941 ttctctttat aagatgaacg cgtcaatttc tggtgctgaa gtcggctgcc agggtgaagt
    24001 tggtgtggcg tgctcaatgg cggcggctgg tctggcagaa ctgttaggtg caagcccggc
    24061 gcaggtgtgc atcgcggcgg aaatcgccat ggagcacaac ctcggtctga cgtgtgaccc
    24121 ggtcgccgga caggtccagg tgccatgcat cgagcgtaac gccattgcgg cagtaaaagc
    24181 ggtgaacgcc gcacgtatgg cgctacgccg taccagcgag ccgcgcgtct gcctcgataa
    24241 agttatcgaa accatgtacg aaacaggtaa agatatgaac gccaagtacc gcgaaacctc
    24301 tcgcggcggc ctggcaatga agatcgttgc ctgcgattaa tcgctctcca gaggcctcgt
    24361 tttgcgaggc ctcttcccga tttctcatcc agccgtagcc tgttccggca tcgaatgtta
    24421 cccttatcgc ctgatcttta agggggttat cgtggctgtt catttgctta ttgtcgatgc
    24481 actgaatctt attcgtcgca ttcatgccgt tcagggatcg ccctgtgtcg aaacctgcca
    24541 gcatgcgctc gatcagctca ttatgcacag ccagccaacc cacgcggtcg ccgtttttga
    24601 tgatgaaaac cgcagtagcg gctggcgtca tcagcgttta ccagattaca aagcgggtcg
    24661 accgccaatg ccggaagagt tgcacgacga gatgcctgca ttacgcgccg cctttgagca
    24721 acgcggcgtc ccgtgctggt caaccagcgg caacgaagcc gatgacttag ccgccacgct
    24781 ggcggtcaaa gtgacacagg ccgggcatca ggcaacgatt gtttcgacag ataaaggcta
    24841 ctgtcagtta ctttcaccga cattacgtat tcgtgattac ttccagaaac gttggctgga
    24901 tgcaccattt atcgataaag aatttggcgt tcaaccgcag cagttgcccg attactgggg
    24961 acttgcgggg atcagcagtt caaaggtacc gggtgttgcg ggaatcggac caaaaagcgc
    25021 cacgcagctg ctggtcgagt ttcagagtct ggaagggata tatgagaatc tggatgcggt
    25081 tgccgaaaag tggcgcaaaa aattagaaac ccataaagag atggcgtttc tgtgccgcga
    25141 tattgcccgc ttacaaaccg atttgcatat cgacggcaat ttacagcaat tgcggttggt
    25201 acggtaacgg cgagccggat gcaaacgatt atcaacacat tgaatttaat gcacttatat
    25261 accggataag acgcggctag cgtcgtatcc ggcattatca catcagcgca tttaccaggc
    25321 ggtatggtaa agctctacaa tatcctcaag cgttgcttca cgcgggttgc caccggtaca
    25381 aacatcatcc agtgccgcct gcgccagtgc cggaatgtct tccttgcgta caccaacatc
    25441 acgcaaatgt ggcggaatac cgacatcacg gttgagagca aacaccgctt caacagcggc
    25501 attacgcgcc tcttccagcc actttcacgc ccataacgcg cgcgatatcg cggtacttct
    25561 caccggtaaa gtcagcgtta tagcgcatga catgcggtaa caggatggcg ttcgcaacac
    25621 cgtgtggagt gttgtaaaac gcgcccagtg gatgcgccat accatgcacc aaccctaacc
    25681 caacattcga gaagcccata cccgcaacat actgcccgag cgccatttct tctccggcat
    25741 ccttatcacc agcaaccgat cctcgcagcg ccccagcaat gatttcaatc gctttaatgt
    25801 gcagtgcatc ggttagcgcc cacgcgccac gggtaatata cccctcaata gcatgagtga
    25861 gcgcatcgac acccgtcgca gctttcagcg ctggaggcat accatccatc atgtcagcgt
    25921 caataaacgc cacctgcggg atatcatgcg gatcaacgca aacaaacttg cgccgtttct
    25981 cttcgtcagt gatcacgtag ttaatggtca cttctgccgc agtacctgct gtggtaggaa
    26041 ttgccagaat cggtacactg ggtttattgg tcggggaaag cccttccagg ctacgcacat
    26101 cggcaaactc cgggttgttg ctgataatgc caatcgcttt acaagtatcc tgtggagaac
    26161 caccaccaat agcgatcagg taatccgcgc cgctattctg gaatacaccg agcccttctt
    26221 tgacgacagt aattgttggg ttgggcacta cgccgtcgta aatcgcccat gccagccctg
    26281 cagcatccat cttatcggtc actttcgcca ccacgccgca ttgcaccagc gttttatcgg
    26341 tgacgatcag cgccttctga taaccacggc gtttcacctc atcggttaaa gccccaacag
    26401 caccccgacc aaaccatgcc gtttcgttca gaatcattct gttagccatc atccttctcc
    26461 ttgttgcttt acgaaattac tcttcaatcc gtaacccata ggttttgaat ttctccagca
    26521 ctacggcaat ctcttcatcg ctcagcactg gcaccgggtc cgtaatcgcc agggtcgtca
    26581 ggtaaagttg cgccagcact tcaacttcat gcgccagcca taacgctttt tccagattca
    26641 cctcacaagc gataagccca tgatgttgta acaaagttgc cttacgattt ttgagagcca
    26701 gcgcaacatg ttcagaaagt tcgcgtgttc caaaggtcgc ataaggcgcg caaggaatag
    26761 aattaccacc tgccgccgca atcatgtagt gaatagcggg gatcggtcgg ttaagaatgg
    26821 aaactgctgt gcaatgaacg gcatgattgt gaacaaccgc gttggcatcc ggtctgcttt
    26881 gataggccgc catatggaaa cgccattcgc ttgaggggag ctttccttcc tcatgtttac
    26941 cgttaccatc aataaaggca atatgcgact ccgtcagttt ttcatatgga atgcctgtag
    27001 gcgtaatcag catcccatcc tgataacgta cactgacgtt ccccgctgtc ccctggttca
    27061 gtcccaggcg ggtcatttcc aggcaagtgt caataatctg acgagcaagt ttatttcgtt
    27121 ccattagcta cctctctctg attcaaaaca gggcaataat gttgttcctt tcacactatt
    27181 gaattagccg tttaattacc caccat
//
LOCUS       DAJTSL010000041        27203 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTSL010000041 DAJTSL010000000
VERSION     DAJTSL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.041"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(174..704)
                     /gene="ppa"
                     /locus_tag="OO433_001559"
     CDS             complement(174..704)
                     /gene="ppa"
                     /locus_tag="OO433_001559"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6050078.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            1014..1970
                     /gene="ytfQ"
                     /locus_tag="OO433_001560"
     CDS             1014..1970
                     /gene="ytfQ"
                     /locus_tag="OO433_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR6050079.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENRIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            2110..3612
                     /locus_tag="OO433_001561"
                     /pseudo
     CDS             2110..3612
                     /locus_tag="OO433_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            3626..4648
                     /locus_tag="OO433_001562"
     CDS             3626..4648
                     /locus_tag="OO433_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6050080.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     EVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            4635..5630
                     /gene="yjfF"
                     /locus_tag="OO433_001563"
     CDS             4635..5630
                     /gene="yjfF"
                     /locus_tag="OO433_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6050081.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(5663..6661)
                     /gene="fbp"
                     /locus_tag="OO433_001564"
     CDS             complement(5663..6661)
                     /gene="fbp"
                     /locus_tag="OO433_001564"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6050082.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            6837..8210
                     /gene="mpl"
                     /locus_tag="OO433_001565"
     CDS             6837..8210
                     /gene="mpl"
                     /locus_tag="OO433_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6050083.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADTANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(8293..8844)
                     /locus_tag="OO433_001566"
     CDS             complement(8293..8844)
                     /locus_tag="OO433_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6050084.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEE"
     gene            8938..10290
                     /gene="pmbA"
                     /locus_tag="OO433_001567"
     CDS             8938..10290
                     /gene="pmbA"
                     /locus_tag="OO433_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6050085.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGVDCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            10474..10860
                     /gene="cybC"
                     /locus_tag="OO433_001568"
     CDS             10474..10860
                     /gene="cybC"
                     /locus_tag="OO433_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR6050086.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(10905..11369)
                     /gene="nrdG"
                     /locus_tag="OO433_001569"
     CDS             complement(10905..11369)
                     /gene="nrdG"
                     /locus_tag="OO433_001569"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6050087.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(11468..13606)
                     /gene="nrdD"
                     /locus_tag="OO433_001570"
     CDS             complement(11468..13606)
                     /gene="nrdD"
                     /locus_tag="OO433_001570"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6050088.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYSNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(14000..15655)
                     /gene="treC"
                     /locus_tag="OO433_001571"
     CDS             complement(14000..15655)
                     /gene="treC"
                     /locus_tag="OO433_001571"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6050089.1"
                     /translation="MTNPPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAVLKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(15705..17126)
                     /gene="treB"
                     /locus_tag="OO433_001572"
     CDS             complement(15705..17126)
                     /gene="treB"
                     /locus_tag="OO433_001572"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6050090.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNARQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAETFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPNYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(17227..18192)
                     /gene="treR"
                     /locus_tag="OO433_001573"
     CDS             complement(17227..18192)
                     /gene="treR"
                     /locus_tag="OO433_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6050091.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPEFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLSRSFPER"
     gene            18377..18430
                     /gene="mgtL"
                     /locus_tag="OO433_001574"
     CDS             18377..18430
                     /gene="mgtL"
                     /locus_tag="OO433_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6050092.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            18571..21267
                     /gene="mgtA"
                     /locus_tag="OO433_001575"
     CDS             18571..21267
                     /gene="mgtA"
                     /locus_tag="OO433_001575"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6050093.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQLSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(21473..21859)
                     /gene="ridA"
                     /locus_tag="OO433_001576"
     CDS             complement(21473..21859)
                     /gene="ridA"
                     /locus_tag="OO433_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6050094.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYETFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAIRR"
     gene            complement(21932..22393)
                     /gene="pyrI"
                     /locus_tag="OO433_001577"
     CDS             complement(21932..22393)
                     /gene="pyrI"
                     /locus_tag="OO433_001577"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6050095.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(22406..23341)
                     /gene="pyrB"
                     /locus_tag="OO433_001578"
     CDS             complement(22406..23341)
                     /gene="pyrB"
                     /locus_tag="OO433_001578"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6050096.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(23345..23479)
                     /gene="pyrL"
                     /locus_tag="OO433_001579"
     CDS             complement(23345..23479)
                     /gene="pyrL"
                     /locus_tag="OO433_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6050097.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(23759..24154)
                     /locus_tag="OO433_001580"
     CDS             complement(23759..24154)
                     /locus_tag="OO433_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6050098.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     SYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(24285..24998)
                     /gene="bdcA"
                     /locus_tag="OO433_001581"
     CDS             complement(24285..24998)
                     /gene="bdcA"
                     /locus_tag="OO433_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6050099.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADKDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMSHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            25069..25662
                     /locus_tag="OO433_001582"
     CDS             25069..25662
                     /locus_tag="OO433_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6050100.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            25807..26259
                     /gene="tabA"
                     /locus_tag="OO433_001583"
     CDS             25807..26259
                     /gene="tabA"
                     /locus_tag="OO433_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6050101.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            26382..>27203
                     /locus_tag="OO433_001584"
     CDS             26382..>27203
                     /locus_tag="OO433_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6050102.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIIKF
                     VMNNPEGERADLSKVEIEITLSTFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNISQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVNNYLLNNKKAT"
BASE COUNT         6487 a         6765 c         7228 g         6723 t
ORIGIN      
        1 tccagcaagt ggctatgaac agcaaaatgg tggtcgggtt tgcttgttct taccgttcaa
       61 gccttgcaac cgctgagaat aatgatggtg gaatagagag tttatttatc gcgggcataa
      121 aaaaaccctt actaaccgaa gcccggcgtt cagggttatt gcgctagaag aacttattta
      181 ttctttgcgc gctcgaagga agcaacgatt tcagctttag cggcttctgc gttttcccaa
      241 ccttcaactt tcacccactt gcctttttcg aggtctttgt agtgctcgaa gaagtgagcg
      301 atttgcgctt tcagcagttc aggcagatcg tttacgtctt taatgtgatc gtattctttg
      361 ctcagcttgc tgtgcggaac cgcaaccagt ttcgcatctt caccggcttc gtcggtcatt
      421 ttcagaacgc caaccggacg gcaacggatc acagaaccag gctgcagcgg gtacggagtc
      481 gggaccagta cgtcaaccgg gtcaccgtcc agagacaggg tgtggttgat gtaaccgtag
      541 ttgcacggat agaacatcgc ggtggacatg aagcggtcaa cgaacagtgc gccgctctct
      601 ttgtcgattt cgtatttgat cggatctgcg ttagccggga tctcaataac aacgtagatg
      661 tcttccggca gatctttacc cgcagggacg ttgagtaagc tcatgtctgt ttcctttaaa
      721 aatatgtggc aaacaagtgc cgagtattat agccaactcg cgccgaatgt ctttgcttgt
      781 tttcgtcttc attttccttt ttcgccagtt ttaagacggt atacagagca ggaaaatcca
      841 taaccgtagc cgcatttttc atagtgagat gaaagcgatt acaaacttgt gattaacgtt
      901 ttatttactt ttttgaagtg tgatgtaacg caatccgtta cataacgaat tgtctatagt
      961 tttttcgcga acatcttttc accaataata attaccccga ctaggacaac cctatgtgga
     1021 aacgcttact tgtagtctct gcagtctcgg cagccatgtc gtctatggcg ttggccgctc
     1081 cattaaccgt aggattttcg caggtcggat cggaatccgg ctggcgcgcc gcagaaacca
     1141 atgtggcgaa aagtgaggcc gaaaaacgcg gaattacgtt gaaaattgcc gatggtcagc
     1201 aaaagcagga aaaccggatt aaagcggtac gttccttcgt tgcacaaggg gtggatgcga
     1261 tctttatcgc tccagtggtt gcgacgggtt gggaaccggt attaaaagag gcgaaagatg
     1321 ccgaaatccc ggtcttcttg ctcgaccgtt ccatcgatgt gaaagacaaa tctctctata
     1381 tgaccaccgt tactgccgac aacatcctcg aaggtaagtt gatcggtgac tggctggtaa
     1441 aagaagtgaa tggcaaacca tgcaacgtgg tggagctgca gggcaccgtt ggggccagcg
     1501 tcgccattga ccgtaagaaa ggctttgccg aagccattaa gaatgcgcca aatatcaaaa
     1561 ttatccgctc gcagtcaggt gacttcaccc gcagtaaagg caaagaagtc atggagagct
     1621 ttatcaaagc ggaaaacaac ggcaaaaaca tctgcatggt ttacgcccat aacgacgaca
     1681 tggtgattgg tgcaattcag gcaattaaag aagcgggcct gaaaccgggc aaagatattc
     1741 tgacaggttc tatcgatggc gtaccggata tctacaaagc gatgattgat ggcgaagcga
     1801 acgccagtgt tgaactgacg ccgaatatgg cgggacccgc ttttgacgcg ctggagaaat
     1861 acaaaaaaga cggcaccatg cctgaaaagc tgacgttaac caaatccacc ctttacctgc
     1921 ctgataccgc aaaagaagag ttagagaaga agaaaaatat ggggtattga gggttgctat
     1981 gcctgatgcc gattcgtagg ccggataagg cgctcgcgcc gcatccggcg atggtgcact
     2041 gaagcctgat gcgacgctta ccgcgtctta tcatgcctac tgggagcacg ctttacaccg
     2101 ggggaaacca tgacgaccga ccaacaccag gaaatcctcc gcaccgaagg attaagtaaa
     2161 tttttccccg gcgtcaaagc gttagacaac gttgatttca gcctgcgccg tggcgaaatc
     2221 atggcgctgc tcggtgaaaa cggggcggga aaatcaacgc taatcaaagc attaactggt
     2281 gtataccacg ccgatcgcgg caccatctgg ctggaaggcc aggttatctc accgaaaaat
     2341 accgcccacg cgcaacaact cggcattggc accgtctatc aggaagtcaa cctgctaccc
     2401 aatatgtcgg tcgctgataa tctatttata ggccgcgaac ccaaacgctt cggccttcta
     2461 cgccgcaaag agatggaaaa gcgcgccacc gaactgatgg catcttacgg tttctccctc
     2521 gacgtgcgcg aaccgctcaa ccgcttttca gtcgcgatgc agcaaatcgt cgccatttgc
     2581 cgggctatcg atctctccgc caaagtgctg atcctcgatg aacccaccgc cagcctcgac
     2641 acctaggaag tggagctatt atttggcctg atgcgtcagt tgcgcgatcg cggcgtcagc
     2701 ctgatctttg tcactcactt tctcgatcag agctatcagg tcagcgatcg gatcaccgtt
     2761 ttacgcaacg gcagtttcgt aggctgtcgg gaaacgcgcg agctaccgca gatcgaactg
     2821 gtaaaaatga tgctggggcg cgagctggat acccacgcgc tacagcgtgc cgggcgaaca
     2881 ttgttgagcg acaaacccgt tgccgcgttc aaaaattacg gcaaaaaagg aacgatagca
     2941 ccgtttgatc tcgaagtgcg ccccggcgag atcgtcggcc tggcaggctt gctgggatcc
     3001 ggacgtaccg aaaccgccga agtgatcttc ggtatcaaac ctgctgacag cggcacggcg
     3061 ttgatcaaag gcaaaccgca aaacctgcga tcgccacatc aggcttcggt actgggcatc
     3121 ggcttctgcc cggaagacag gaaaaccgat ggcatcatcg ctgccgcctc ggtgcgggaa
     3181 aatatcatcc tcgctctcca ggcccagcgc ggctggctac gacccatttc ccgcaaagag
     3241 cagcaagaga ttgccgaacg ctttatccgc cagcttggca ttcgcacacc ttcaactgaa
     3301 caaccgattg aatttctctc cggcggcaat cagcaaaaag tgttgctttc acgttggcta
     3361 ctgacccgac cgcaatttct gatcctcgat gagccaaccc gtggcattga tgttggtgcc
     3421 cacgccgaga tcatccgcct gattgaaacg ctatgcgccg atggtctggc gctgctggtg
     3481 atctcctccg aactggaaga actggtgggc tatgccgacc gggtgatcat catgcgcgat
     3541 cgcaaacagg tggcggagat ccggctggca gagctttccg ttccggctat catgaacgcc
     3601 attgcggcgt aaggagaaca gtgtgatgcc tcaatctctt ccggacacca ctacgccaaa
     3661 aaggcgcttt cgctggccga cggggatgcc gcagctggta gcactattgc tggtgctgct
     3721 ggtcgacagc ctggtagccc cgcatttctg gcaggtagtg cttcaggatg ggcgtttgtt
     3781 cggtagcccc atagacattc ttaaccgtgc agcccccgtt gcgttactgg cgattggtat
     3841 gacgctggtg atcgccaccg gtgggattga tctctccgta ggggcggtga tggctatcgc
     3901 cggagccaca acggctgcga tgacggtcgc ggaagtcagc ctgccgattg ttttgttaag
     3961 cgccctaggc accggcatcc tggcgggatt gtggaacggc atactggtag cgatcctcaa
     4021 aattcagcca tttgtcgcca ccctgatcct gatggtcgcc gggcgcggcg tggcgcaact
     4081 gatcacctcc ggacagatcg tcacgtttaa ctcgccggat ctctcatggt ttggcagtgg
     4141 atcgctgttg ttcctgccaa cgccagtcat tattgcggta ctgacgcttc tcctgttctg
     4201 gctgttgacc cgcaaaacgg cactggggat gtttatcgaa gccgttggta tcaacattcg
     4261 ggcggcaaaa aatgccgggg taaacacgcg gatcatcgtc atgctcactt atgtgttgag
     4321 cgggctgtgt gcggcgattg cgggcattat cgtggcggcg gatattcgcg gtgccgatgc
     4381 caacaacgcc ggattatggc tggagctgga cgccattctc gcggtggtga ttggcggcgg
     4441 atcgctgatg ggcgggcgct ttaacctgct gctctcggta gtgggggcgc tgattattca
     4501 ggggatgaac accggaattt tgctttcggg ctttccaccg gagatgaacc aggtggtgaa
     4561 agcggtggtg gtgctttgcg tgctgattgt ccagtcgcaa cgctttatca gtctgattaa
     4621 aggagtacgt agccgtgata aaacgtaatt tgccactgat gatcaccatc ggcgtctttg
     4681 tgctgggcta tttctactgc ctgacgcagt ttcccggttt tgcttccaca agagtgatct
     4741 gcaatatcct gaccgataac gcttttctgg ggattattgc cgttggtatg acctttgtga
     4801 tcctctctgg cggtatcgat ctctccgtcg gttcggtgat cgcctttact ggcgtgtttc
     4861 tggcaaaagt ggttggcgat ttcggcctct cgccgctgct ggcgtttccg ctagtgctgg
     4921 tgatgggctg tgccttcggc gcatttatgg ggcttctgat cgacgccctg aagatcccgg
     4981 catttatcat tacgcttgcg gggatgttct ttttgcgcgg cgtcagctat ctcgtttcgg
     5041 aagagtcgat tccgataaac catcccattt atgacacgct ctcaagcctt gcgtggaaaa
     5101 tccccggcgg cggtcgcctt agtgcgatgg ggctgttgat gctggcggtg gtggttatcg
     5161 gtattttcct tgcgcatcgt acccgttttg gcaatcaggt gtacgctatt ggcggcaacg
     5221 cgacgtcggc gaacctgatg gggatttcta ctcgcagcac tactattcgc atttatatgc
     5281 tctccaccgg actggcaaca ctggcgggga ttgtcttctc gatttatacc caggccggat
     5341 atgcactggc gggcgtaggt gtggaactgg acgccatcgc gtcggtggtg attggcggta
     5401 cgttgttaag tggtggcgtc gggacggtgc ttgggacgct ttttggcgtg gcaattcagg
     5461 gactgatcca gacttacatc aactttgacg gcacgctgag ttcctggtgg acgaaaatcg
     5521 ccatcggtat tttgttgttt atttttatag cattacagcg tgggttaacg gtgctgtggg
     5581 agaatcgtca gagttcgcca gtgacaagag tcaacattgc gcagcgataa aaatgcctct
     5641 ccgtgtggag aggcgcagga gattacgcgt ccgggaactc acggataaag cgttcgacat
     5701 cttcaaccat atggtcgttg ccgacaaaga acgaacggcg ctggtgcaga gtttccggga
     5761 tgatatccag aatacgctct ttgccatcgc tcgctttacc gcccgcttgt tccgccagga
     5821 atgccatcgg gttgcactca taaagcaaac gcagtttgcc gtccgggtgg ctggcggtgc
     5881 ttgggtagag ataaataccg cctttcagca ggttacggtg gaaatccgca accagtgaac
     5941 cgatataacg tgaggtatac gggcggttgg tggatttatc ttcttcctgg cagaatttaa
     6001 tgtacttctt caccccgttc ggaaacttaa tgtagtttcc ttcgttgatg gagtaggttt
     6061 tgcctttctc cgggaagcgc atccgttcct ggcacaggca gaaaacgccc agcgaaggat
     6121 cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac cagcatggta gaggagccgt
     6181 ataccacgta acctgccgca acctgtttgt taccaggctg gaggaaatct tcttccgtta
     6241 ccggcgtgcc gacgggcgta actcggcggt agatggagaa aatggtaccg acagagacgt
     6301 taacatcgat gttggacgag ccatccagtg ggtccatcag caccacgtat tttgcatgtt
     6361 cacagccttc aaagacaaca atctcatctt cttcttcaga ggcaatgcct gcaacgatat
     6421 cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa caagtcgagt ttttgctgaa
     6481 cctcgccctg cacgttctca gcaccgctgg cacccaggat atcaaccagt cctgctttgt
     6541 tgatatcgcg atggataatc ttggcgccca gttttattgc cgacagcaaa gcagtgagct
     6601 caccggtagc atgagaaaac tcgtgctgct tttcgacaat aaattcacct aacgttttca
     6661 taaaactttc cctgcaatgt ttatggagta aagcgaccgc aacaatctta acaaataatc
     6721 tcaatgttgc gctcaggtga atcgcgccag caaattacgg attatcctga aatgcgtttc
     6781 tcacttgccc gacatatgcg taaaatgagc ggcagattaa aaaaggatag tgacgtatgc
     6841 gcattcatat tttaggaatt tgtggcacgt ttatgggcgg tctggcgatg ctggcgcgcc
     6901 agttaggcca tgaagtaacg ggttcggacg ccaatgtgta tccgccgatg agcaccttac
     6961 ttgagaagca aggcattgaa ctgattcagg gttacgatgc cagccagctc gatccgcagc
     7021 cggatctggt gattattggc aacgccatga cccgtggaaa tccgtgtgtg gaagcggtac
     7081 tggaaaaaaa catcccttat atgtcaggtc cacagtggct gcacgatttt gtgctgcgcg
     7141 accgctgggt gctggccgtt gccggtacac acggcaaaac caccaccgcg ggaatggcga
     7201 cctggattct ggaacagtgt ggttacaaac cgggatttgt gatcggcggt gtgccgggga
     7261 actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt tgttatcgaa gcggatgagt
     7321 acgactgcgc cttcttcgac aaacgctcta aatttgtcca ttactgcccg cgtacgctga
     7381 tcctcaacaa ccttgagttc gatcacgccg atatctttga cgacctgaaa gcgatccaga
     7441 aacagttcca ccatctggtg cgtatcgttc cggggcaggg ccgtattatc tggccggaaa
     7501 atgacatcaa cctgaaacag accatggcga tgggctgctg gagcgagcag gagctggtgg
     7561 gtgagcaggg gcactggcag gcgaaaaagc tgaccactga tgcttccgaa tgggaagtct
     7621 tgctggatgg cgaaaaagtg ggcgaagtga aatggtcgct ggtaggcgaa cataatatgc
     7681 ataatggcct gatggcgatt gcggcggccc gccatgttgg cgtagcgccg gcagataccg
     7741 ctaacgcgct gggttcgttt attaatgctc gtcgccgtct ggagttgcgt ggtgaagcga
     7801 atggcgtcac ggtatatgac gattttgccc atcacccgac ggcgattctg gcaacgctgg
     7861 cggcgctgcg tggcaaagtt ggtggtacag cgcgcattat tgctgtgctg gaaccgcgct
     7921 cgaataccat gaaaatgggg atctgcaaag acgatttggc accttcatta ggtcgtgccg
     7981 atgaagtctt cctgctgcaa ccggcgcata ttccgtggca ggtggcagaa gtggcagaag
     8041 cctgcgttca gcctgcacac tggagtggcg atgtggatac gctggcagat atggtggtga
     8101 aaaccgctca gcctggcgac catattctgg tgatgagcaa cggcggtttt ggtgggatcc
     8161 atcagaaact gctggatggt ctggcgaaga aggcggaagc tgcgcagtaa ttcggcctca
     8221 gcctgagata gcattgccgg ataaggcgtt tacgccgcat ccggcaaacc atactcacat
     8281 caacaacgaa aattactctt cattctccgc cagttcgcgc agatactgga aaatctggcg
     8341 tgcggatttc ggcggcttat tcccttcttt ctctttcttc gcgttgcgga tcagagtacg
     8401 cagttgctga cgatcggcat cgggccacag atttaatacc tcagcgatgg cgtcatcacc
     8461 ttgatcgatc agacgatcgc gcaggttttc cagtttatgg aacagcacca cctgctggtt
     8521 gtgacggttt ttcagcttat ccagcgcctg acggatcggt tctacatcgc gctggcgcag
     8581 catcttaccg atgagctgca actggcggcg gcgaccttcc atcttaatac gctgggcgag
     8641 ctcaatagcc gcgcgcagat ccgcatctaa cgggatctta tccagcgcgt ttttccccag
     8701 atcaacaatt tccgcgccaa ggcgttttag ctcctcggca tcacgtttaa tttcactttt
     8761 actgacccag ataatttcat cgtcttcgtc ttcgatgtca tcaccgggaa cgtcgtcgag
     8821 ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa aaagaggcta atgttaccag
     8881 ttaagatgcg cactgaaaaa cggttctctg ttagacttca gagaaactct ctacattatg
     8941 gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct ggaagaagca
     9001 gtttccactg cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt tgccgtcagc
     9061 aagaccaccg gcattagcgt aagcacgcgt tatggtgaag tggagaatgt tgaattcaat
     9121 agcgatggcg cgctggggat cactgtttat caccagaacc gcaaaggtag cgcatcatcc
     9181 accgatttaa gcccgcaggc cattgcccgt actgtacagg cggcgctgga tattgcccgt
     9241 tatacctcgc cagatccctg tgccggcgtg gcagacaaag agctgctggc ctttgacgca
     9301 ccagatctcg acttgttcca ccctgcggaa gtttccccgg atgaagccat tgaactggcg
     9361 gcccgcgcag aacaggcggc attgcaggcg gacaaacgca tcaccaatac cgaaggtggc
     9421 agctttaaca gccactacgg tgtcaaagtt tttggcaaca gccacggcat gttgcagggt
     9481 tactgctcaa cgcgtcattc gctctccagc tgtgtaattg ccgaagaaaa tggcgatatg
     9541 gagcgtgatt acgcctacac cattggtcgt gcgatgagcg atctgcaaac gccagagtgg
     9601 gttggggtcg actgtgctcg ccgtacttta tcgcgcctgt caccgcgtaa actctccacc
     9661 atgaaagcgc cggtcatttt tgccaatgaa gtggcaaccg ggctttttgg ccatctggtg
     9721 ggggcgatag cgggtggatc ggtttaccgt aaatctacct tcctgctgga ttcgctgggt
     9781 aaacaaattc tgccggactg gctgaccatt gaagagcatc cgcatctgct gaaagggctg
     9841 gcgtcgacgc cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat tattaaagat
     9901 ggcatcctga ctcagtggct gctgaccagc tattcggcgc gaaaactggg gctgaaaagc
     9961 accggacatg cgggcggtat tcacaactgg cggattgccg gacaaggtct aagcttcgag
    10021 cagatgctca aagagatggg caccgggctg gtggtgacgg aattgatggg ccagggcgtg
    10081 agtgcaatta ccggtgatta ttcccgtggt gcggcgggct tctgggtaga aaacggcgaa
    10141 attcagtatc cggttagcga aatcaccatc gcaggtaatt taaaagatat gtggcgcaat
    10201 attgtcaccg tcggtaacga tattgaaaca cgcagtaata tacagtgtgg ttctgtgctg
    10261 ttgccggaga tgaaaatcgc cggacagtaa aaaaactggc gcgacctgtc gcgctataaa
    10321 ctaaaaaatt acacaaaatc attcgcactg catcgagacg gaaagtgaat gagggccggg
    10381 gagcgtactc gcagtacgtg acccggtcga atgagcgtag tcgtcgatga ggcagtgtga
    10441 atgatgaagt gtaaataaaa aaggaagtga gcaatgcgaa aaagcctgtt agctattctt
    10501 gcagtctcct cgttggtatt cagttcggcg tcgtttgccg ctgatcttga agacaatatg
    10561 gaaaccctca acgacaattt aaaagtggtc gaaaaagccg ataacgcggc gcaagtcaaa
    10621 gacgcgttaa cgaagatgcg cgccgcagcg ctggatgcgc aaaaagcaac gccgccgaag
    10681 ctcgaaggca aatcaccgga cagcccggag atgaaagact tccgtcacgg tttcgatatt
    10741 ctggtcggtc agattgacga cgcgctgaag ctggcaaatg aaggtaaagt aaaagaagcg
    10801 caggctgccg cagagcaact gaaaacgacc cgcaacgcct atcaccagaa gtatcgttaa
    10861 ttcctcattt ccctgttgcc tgcactcagg taacagggaa cttatcatcg caaatgatgc
    10921 accacctgat tgctgctgcc gcgccagatc agggaagggt cttttaaatc ctgcacaaat
    10981 ttgccgtcga ccagcacgtt aatcaaatca acaacctgca tttgcgctgc gttgagttcg
    11041 tcgagtttat agcctgtcca cacccagatg tctttacccg gacactcggc gcggatgcgt
    11101 tgtaccagtt tcagaatatc cggcacgttt tgcggatgca gcggatcgcc gccggagagg
    11161 gaaataccct ggcgtttgat acgagtgtca ttcagatcgt taatgatctg gtcttccatt
    11221 gctttagtaa atggctgacc ggaatttacc cgccatgtgc ttttgttata gcaaccgggg
    11281 cattcatgaa cacacccgga gacaaacaga gtgcaacgag tgccggggcc gttgacgatg
    11341 tcgacaggat agtactgatg ataattcatt atttcgcctg gcttatcagt ccgcgtgtag
    11401 actgcatccg gtaatcaatg cctgatgcga cgctttcaca tcacatccgg catttgctta
    11461 gtgcagatca acctatctgc ccatttccca aatgtttaac gcggcgctta acttcttcct
    11521 gcttgccggc gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag
    11581 ttaccgacac acgggaggcg tcatggttac cacatttcgg gcaggtgaag cctttgctgg
    11641 tgcactcgaa ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc
    11701 cgtaatacgg tacatgctga tagctgtaat cccagacatc ttccagcgcc ttcaggttgt
    11761 gctgaatgtt tgaatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag
    11821 gcgcttcaaa gtcgatcttg tcgtacgggt tcaccttctt ctccacatcg aggtggaaac
    11881 tgttggtgta gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga
    11941 gacggcagaa gcggtcgcac aggttttcac tcggcgtgct gtagagactg aaaccataac
    12001 ccgtttcttc cttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt
    12061 tcgcgcgaag ctgctcgttg tcgtaaacat gctcgccgcc gaacagcgcg ttaatggttt
    12121 cgtggatgcc gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa
    12181 catcatcgtc agcattgaga cgcacgccac aagcaccttc catatagagg atcggggcca
    12241 cgcgcgcttt cacgccttcg agacgagcga tacgggtcat cagcgcctta cgtgccagca
    12301 ccagacgttc atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa
    12361 tacgcggcag gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc
    12421 cgttttcatt ttcccacacg ccgaggaagc tgcggcagcc catcggagtt ttaaacgaac
    12481 cggtgacttt cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc
    12541 actccagcgc cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat
    12601 cgcgaatcgc aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg
    12661 cgatacggtt acgcaggata gattcctgaa tcaggcgcga ctcccagctg gtgcccagac
    12721 caaaaccaaa ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt
    12781 actccagcga ctggaaggca tcgtagcact ctttaatggt acgagagtta gcgtagcctt
    12841 cggcgtccgg gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtga
    12901 caaacggtgc cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg
    12961 cgacctgagc aataatctgc gcagttacag cggttgccgt agagatcgac ttcggcggtt
    13021 caatctcggc gttccccatc ttaaagccct gggtcagcat gcctttcagg tcgatcaaca
    13081 tgcagttgaa catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac
    13141 gctcatgtgc ctgcaccacg tcacgcggca gcaggtgctg acgtgcatag tgtttagcca
    13201 cgatcccggc cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt
    13261 cgttgagtaa cgaggcgttg gtctgctcga ccagaccacg gatctcctga ttcaggcgac
    13321 cgcgtttttc acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt
    13381 gtttgtatgg acctgacatc agctggtttt caacagcggt ctggatctca ttgatatcca
    13441 cctggttgcg gccctgcatc tgctcgctga caactgcagc aacagtggcg caataatcgg
    13501 catcatcgac ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt
    13561 taaacggcac tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt
    13621 tttaagaaca ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt
    13681 ttccggtgct ttccgcaatc gccatccgct tttccacaac gccgcacctt tttcgtgggg
    13741 agctgtagta gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag
    13801 tctatatata gtgctttgca tcaaggatgt ttgaactttt tttgatgtag ctcaaagtaa
    13861 aaagcagagc gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc
    13921 tggttgaaaa ataaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt
    13981 accggggctt agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat
    14041 tcatggcaca gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc
    14101 cgcgcatttg ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca
    14161 aggtttgccc cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat
    14221 cctggtaatt gccccatgtc aggatggctt cctgcttacg cagtgcgatt aacttttggt
    14281 aggtgtaaaa caccgaggaa tcatcggcca gcgcggcttc tacgttgatt tgttgatagt
    14341 tatcacccag gccaatccac ggttcgccag ccgtaaatcc ggcattatcg ccgttgctcc
    14401 attgcatggg cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt
    14461 cggcatcacg accatcgttg cgcagctcgg caaacatatt gaggctctct acgtcgcgat
    14521 agtcagtaat gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat
    14581 acggcgttcc ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt
    14641 attcaccttc atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca
    14701 aggcattcca tgctacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg
    14761 ccacaaagtc aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt
    14821 gatgaaaatt aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat
    14881 gctcaaggct ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat
    14941 cgcggttcat ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc
    15001 cgtcgccatc caggtcatca gggaaacgcg ggtctttgga gatcagattc accacatcca
    15061 ggcgcaaccc gtcgacccca cgatcggccc agaactcaca gacttttttc agcactgcgc
    15121 gtaccgctgg attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt
    15181 actgttcgct ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt
    15241 tcggtggcgt ttctggttct ccatcgcgcc agatataaaa ctggcggtaa gggctttctt
    15301 tgttcagcgc ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga
    15361 gaatgatacg aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg
    15421 tgccgtaggt gggatcaatc gccgtatagt tcgctacgtc gtaaccgtta tcgacctggg
    15481 gagagacata aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca
    15541 ggcgttggat aacgccacgt aaatcgccgg taccgctacc cgtggtgtcc tgaaaactct
    15601 ttggataaat ctggtagata acgccgtttt gccaccagtg gggaggatta gtcattacgt
    15661 tattcctgca aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc
    15721 gtgcccaggc ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg
    15781 gcgatagcca ttgccagcgc aaacacctgc cagtagttcg gttgaatcga gagaattccc
    15841 ggcaggccgc ctacgccgat gccattcgcc ataacgccgt tcaggccgca tagcaatcct
    15901 gccagaccag aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg
    15961 tacattgcag gctcagtgac cccaagccag gcggagatcg ccgcaggcac ggagatctcg
    16021 cgttcattgt gcttgcggct ggaaatgata atgccgatca cggcggagcc ctgagcgata
    16081 ttcgacagcg caatcagtgg ccacactggc gtaccaccca tgctttgaat catctgcaaa
    16141 tcaatagcaa gcgtggtctg gtgtaccccg gtgatcacca gcggggcgta caggaagcca
    16201 aacaatgccg cgccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca
    16261 acgccatcgc caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg
    16321 aggatcagcg aacagacggg caccaccacc agatagaggt aatctggcac gatgcgttta
    16381 aggcgagttt caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc
    16441 tgatagccca ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc
    16501 ccgagcagat aagcgttcat cagttggggg gaaaccagtg tcacgccaag cacgatacca
    16561 aggatcggtg tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag
    16621 aagaagatcg cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac
    16681 atttgcgcca gcgtttgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg
    16741 aggatcaaac cgccgctaat caacgcgggc agcaacggga agaaggtctc tgcgaaatga
    16801 gagatcaact gctcatgcca tttcatattc tgccgggcgg ctttttttac ctgctcttta
    16861 tcaacctgcg cctgtccggt tgacgctatc agtgcttgat agtagtcacc cacgttggtg
    16921 ccaatcacca cctgaaattg cctggcattg gtgaaacagc ctttcaccat ggggagttgc
    16981 tcaatttctt tcggtctggc attggccggt tggttgagga caaagcgtag gcgagtaata
    17041 cagtggctca ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata
    17101 tccgtttggt ttattttgct catcataaag ccccatggca gatgacattt ttggttggct
    17161 gcagaatata ttgcgcggaa aaatttaaaa acgggaacgt tccctaaacg cagcgaagat
    17221 cacaatttat cgttcaggaa acgatctgga cagggtggcg gggatgatga tttgttgcgg
    17281 ttcgctgcgc ccggttacct gtgcgatcaa ctggcaggcc gcctggcgtc cagcttcggc
    17341 gtaaccggga tctacggtga cgatctccgg atggaggaat ttcattaacg gcgtattacc
    17401 gacgctcgcc agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag
    17461 tgccagcgtg tcggttgcgc acagtaaggc ggtagtttca ggcgtaatca cttttgctac
    17521 gttctcatag ccttgcttca tagcaaggcc tggcagagcg gcaacgggat gcagtttatg
    17581 cgctttgcag aacgccaggt aggcttcgtg acgtcgctta ccggtcgtca cgtcactgtg
    17641 cggcacgccg agataactga tattacgatg cccctggtca tacagccgtt gcatcaggat
    17701 cttgattgcc ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag
    17761 cagaaccagc gatgactgcc agtgggctaa catttcttct gttatgccgg taaaaccgaa
    17821 cagcactacg ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg
    17881 cggggaaaac tgactttcca tcatgattgg gtcgtaacct tgttcataga actctggcag
    17941 catggtttga acggcgagat tttctgacaa cgaatccagg cgggtaacaa tgatggcgac
    18001 cactttatcg ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg
    18061 attcatcact gcttcaacac gctcgcgggt gcgctggctc acgccgcttt cgttattcag
    18121 cacccgggaa actgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag
    18181 ccgattttgc atcctgttgt cctgtaacgt gttgtttaat tatttgagcc taactttacc
    18241 cgtgcattca gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta
    18301 tatcgccata gatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc
    18361 acttaccgga ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact
    18421 tttccggtaa gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaac
    18481 gtccctgctc agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt
    18541 actgtttcag acagtgcgga gggactcctt atgtttaaag aaatttttac ccggctcatt
    18601 cgccatttac cttcccgtct ggttcatcgt gatccattgc ctggcgcgca gcagacagtg
    18661 aatacggtgg tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa
    18721 gaagaattgt ggaaaacgtt cgacacccat ccggaagggt taaatcaggc ggaagtggaa
    18781 tctgcccgcg aacaacatgg tgaaaataaa ttacccgcac aacaactgtc gccgtggtgg
    18841 gtacatttat gggtctgcta tcgcaacccc tttaatattt tactcaccat tctcggcgct
    18901 atttcttacg ccacggaaga tttatttgct gcgggcgtta tcgcgctaat ggtcgctatt
    18961 tctacgttgc tgaactttat tcaggaagca cgttccacta aagcggcaga tgccctgaaa
    19021 gcgatggtca gcaataccgc gacggtgctg cgcgtaatta acgacaaagg cgaaaatggc
    19081 tggctggaga tcccgatcga ccagctggtg cccggcgata ttataaaact ggcggcgggg
    19141 gatatgatcc cggcagattt acgtatcttg caagcgcggg atctgttcgt cgctcaggcg
    19201 tcgttaaccg gtgagtctct gcccgtggaa aaagccgcta ccactcgcca gccggagcac
    19261 agcaatccgc tggagtgcga cacgctgtgt tttatgggca ccaccgtggt gagcggcacg
    19321 gcacaagcaa tggtgattgc tacaggtgcc aatacctggt ttggtcaact ggcggggcgt
    19381 gttagtgagc aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgtcagtatg
    19441 ctgctgattc gctttatgct ggtgatggca ccggtggtgc tgttaatcaa tggctacacc
    19501 aaaggcgact ggtgggaagc ggcgctgttt gcgctttcgg tcgcggtagg cctaacgccg
    19561 gaaatgttgc cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa
    19621 cagaaagtga tcgtcaaaca tctggatgct attcagaact ttggcgcaat ggatattctg
    19681 tgcactgata aaaccggcac cctgacgcag gataaaattg tgctggagaa tcataccgat
    19741 atctccggta aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattatcag
    19801 accggactta aaaacctgct cgatacagcg gtgctcgaag gtacggatga agagtcagcg
    19861 cgctcgctgg ccagtcgttg gcaaaaaatc gatgagattc cgtttgattt cgagcgtcgc
    19921 cggatgtcgg tggtagtggc agaaaatacc gagcaccatc agctggtttg caaaggtgca
    19981 ttgcaggaaa tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtgccgctc
    20041 gatgacatca tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg
    20101 cgcgtggttg cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg
    20161 gatgaatccg acctgatcct cgaaggatat attgcttttc ttgatccgcc aaaagagaca
    20221 accgctccgg cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc
    20281 gacagtgagt tagttgcagc gaaagtgtgc catgaagtgg ggctggacgc gggagaggtg
    20341 gtcattggta gtgatattga aacgctatct gacgacgaac tggcaaatct tgcacagcgc
    20401 accacgctgt ttgcccgcct gacgccgatg cataaagaac gcattgtgac cttactgaag
    20461 cgcgaagggc atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc
    20521 gcggcggata tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat
    20581 atcatcctgc tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc
    20641 actttcgcca acatgctgaa atacatcaaa atgacggcga gttctaactt tggtaatgtg
    20701 ttcagcgtgc tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg
    20761 cttattcaga acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac
    20821 gacgagcaaa ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatgatc
    20881 ttcttcggac cgatcagctc gatcttcgat attctgacgt tttgcctgat gtggtgggta
    20941 ttccatgcca acacgccgga aacgcaaacg ctgttccagt cgggatggtt tgtggtgggc
    21001 ttactgtcgc aaacgctgat tgtgcatatg atccgcaccc gccgtgtgcc gtttattcag
    21061 agctgtgcat cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca
    21121 ttgccgtttt caccgctggc cagttatctg caattacagg cgttgccgtt aagctatttc
    21181 ccgtggctgg ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaaggattt
    21241 tatagccgtc gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga
    21301 ttgcagacaa agtgagtttt gttcatgccg gatgcggcgt aaacgcctaa tccggcctac
    21361 aaattcaatc aattgcagga atcacgtagg cctgataagc gtagcgcatc aggcgatttt
    21421 gcgtttgtca tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac
    21481 gaatagcgat cgcttcgatc tcaatcttta cgtctttcgg cagacgggca acttcaacgc
    21541 aagaacgtgc cgggaaggtg gcgttgtgtt cggtgaagaa ggtttcgtaa gtggcgttta
    21601 cggttgcgaa gtcgttcaga tcttttacaa acacggtagt tttaacgatg tcgcccactt
    21661 tcagaccagc ggcttcgacg atcgctttta cgttatccag cgactgacgt gcctgtgcag
    21721 cgacgtctgc cggtacttcg cccgttttcg gatttaccgg gatctgaccg gaggtgatga
    21781 tcatatttcc cagatcaacg ccctgtacgt aaggaccgat agctgctggt gcattttccg
    21841 tcgcgatagt tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta
    21901 tagggagcca gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa
    21961 actctttttc acagtatttg catttgagcg cgatgtcatt ggcgcgtttt cgcacggcaa
    22021 agctggatga aaccggttcg gcatggctga tacagttgct gttcgggcag accaacacat
    22081 tgtcgatgcg ctctggcaga ctggggcgcg atttacccac cacttcatag ttgtcgatac
    22141 ggttaaccgt ggcttgcggc gcatacaatg ccagttgatc tacctgatct tcactcaaaa
    22201 aggtattttc gattttgatc agatctttgc ggcccatctc gccagaaggc aggttcagac
    22261 caatggtgat gcgctgatcc gtttcggtca gcttgaacag actcaacagc ttaaaaccga
    22321 tctgggcggg gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat
    22381 tatcgtgtgt catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca
    22441 gtaacgcctg gcgagcgaaa atcccgttgc ctgcctgctg gaagtaccag gcgtgtggcg
    22501 ttttatcaac atccgtcgca atttcatcaa cacgcggcag cggatgcagt actttcatat
    22561 tggctttggc gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt
    22621 attcggatgg gtccaggcgc tctttttgca cgcgggtcat atacaggatg tctacttccg
    22681 ccatcacttc ttcaatagag ctgtgcagac tccatgcgat ccctttttca tcgagcatat
    22741 ccagaatgta ttgcggcatt gccagcgcgt ccggcgcgat gaagtaaaaa cggttgccgt
    22801 cgaacttcgc taacgcctga gtcagggagt gaacggtgcg gccatatttc aggtcaccaa
    22861 ccattgcgac gtggagattg tccagacgcc cctgggtttc ctgaatagtg aataagtcca
    22921 gcaaggtttg cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc
    22981 cggaaaactc ggtggccagg cgcgccgcac cttcctgcgg atgacgcatc actatcgcat
    23041 cgacgtaagt gctgataacc gaaatggtat cggccagcgt ttcgcccttt ttacccagtg
    23101 atgtattggc gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt
    23161 cgaaagagag gcgggtacgg gtagaggctt cgaagaagca gctggcaatg actttgtgct
    23221 tcaacagctc tggttgcggg tttgctttca gttttgccgc tgttgccagc accagattaa
    23281 ggtcatcgcg actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca
    23341 tcttttatct cctgacgcct gggcaaaaaa aagcccctcg attgaggggc tgggaatggg
    23401 tgatcaacgg gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat
    23461 gtcgaacaca ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatcgtca
    23521 gaagcggtgc aagcaattga tgcaatattt tgtcagcgca aacggtttat ttgaatttaa
    23581 agttaaaata tatgcatttt tatttactgt gattctgggg ggaactgtcg ttcggtatca
    23641 ataattgcag acatttcctg cttttttctt tttcactttc acgcaatcag atatgcattt
    23701 tattccattc atccgactta tagggcggag tgtgaaaacg aacggctagc actattgctt
    23761 agagctcagg gatgcgcgct atcactttaa tttcaaaatc aaagcctgcc agccatgtaa
    23821 cgcccaccgc cgtccaggtt ggataagatg gcgcgctaaa tatttcattt ttcaccgtca
    23881 tgatgtcttc aaattgtttt tctggatcgg tatgaaagct cgtaacatca atgatatcgt
    23941 caaaagtgca tcccgcagct gccagggtcg catgcaaatt atcaaaggcc agtctgactt
    24001 gttgctgaaa atcgggttct ggtgttccgt cctctcgact tcctacttgc ccggaaacaa
    24061 acagcaaatc gccggaacga atagccgcag aataacgatg ctcagcatac agtgaatgtc
    24121 gtccagcagg gaaaacagcg gttctttcta ccatttggtt atcctcaaga tttacgacat
    24181 gaacagaaga tttatattta ccaggagccg cttttagcgg acgacgtgag taaacaaaac
    24241 ccagacatca tgggaaatgg ctgggcttaa ttgagcgtag tcggttatgc gccaaacgcg
    24301 ccatcaatgg tatgcatcgc gccggtaaca aaactggctt ctggccctgc taaccatgcg
    24361 accataccag cgacctcttc cggttgccca tgtcttttga tggccataaa actatgcgac
    24421 atatcgcgca ttggcccgtt ggcgggatta gcgtcggtat caattggccc tggctggacg
    24481 acgttgatgg tgatcccacg cggtccgaaa tcacgggcca gcccgcgcgc catgccttgc
    24541 agggcagatt tactggcggc ataagcagcc atgcctgcaa caggcatacg atcgccattc
    24601 acggagccga tgattaagat gcgtccgcct tcgggcatct gccgggcggc ttcaacagag
    24661 gcatggtaag gagcatgaat attgattttg aaaaggcgat caatatcgtc ggcatttaat
    24721 tccagggcat cgccaaagac gccaatacct gcatttacca ccaggatatc caatgcgccg
    24781 ctcttacgaa cgacatcaat aacagcgtct ttgtcagcac tatctgtgaa tactgctgtc
    24841 gctccagtct cttgtgccag gcgttcagcg gcatctttcg accccgcata ggtgaatcgt
    24901 acattggccc catcggtgac gaaacgacgt acgatagcgg caccgattcc acggctacca
    24961 ccgaggatga gaactgtctt acctgtaaaa gcgcccataa ggactccttg atttattatg
    25021 taacatgcat tacaaaactg ttttaacttt ctgtcaacaa gttttgtaat ggtcactaaa
    25081 aaacaatctc gcgttccagg tcgtcccaga cgtttcgctc ctgagcaggc agtctctgcg
    25141 gcaaaagtgc tttttcacca aaaaggtttc gatgctgtca gtgttgctga agttactgat
    25201 tatcttggta ttaacccccc gagcctctac gcggcttttg gcaataaggc tgggttattt
    25261 agccgtgtac tcaatgaata tgtcggtacg gaagctattc cgcttgccga tattcttcgt
    25321 gatgatcgtc cggtagggga gtgcctggct gaggtattaa aagaagcggc gcgccgatat
    25381 agccaaaacg gcggctgcgc tggctgtatg gttcttgaag gcattcatag tcatgatcca
    25441 caagcgcgtg atattgccgt tcaatattat cacgccgcag aaacgaccat ttatgactat
    25501 atcgccaggc ggcatccaca aagcgcacaa tgtgtgactg attttatgag taccgtgatg
    25561 tcagggcttt ctgcgaaggc acgggagggg cactccatcg aacaactctg tgcaacagct
    25621 gcactggcgg gggaagcgat aaaaactatt ctcaaggagt gatgcgggcc ttgacccgaa
    25681 aggcgggaac gcgcctgccg ataagttgtg ataagacaat aattcacgcg ttaaggctag
    25741 cggaattgat catcttttcg tataacgatg gaaatgaaac gttgttttaa ttaaggagtg
    25801 gaaaagatga tcatcggaaa tattcataat cttcagccgt ggctacccca ggagttacgc
    25861 caggcgattg agcatatcaa agcacacgtt acggcagaaa cgccaaaggg caagcacgat
    25921 atcgaaggca atcgtctgtt ttatcttatc tcggaagata tgaccgagcc gcacgaagct
    25981 cgccgtgcgg agtaccatgc ccgctatctc gacattcaga ttgtgttaaa aggtcaggaa
    26041 ggcatgacct tcagcacgca acctgcaggc accccggata ccgactggtt agctgataaa
    26101 gacatcgcat ttttgccgga aggcgttgat gagaaaacag ttatccttaa tgaaggtgat
    26161 tttgttgtgt tttatccggg ggaagtgcat aaaccgctgt gcgcagtggg cgcgccagcc
    26221 cgggttcgca aagcagtagt gaagatgctg atggcgtgat gacttttcgc cgtaaataac
    26281 tccaggttta cggcgagttt gtgaaaagag cgttttttga tatttttttg tgagtaaaat
    26341 ttgtaatgct tagacgttct tattaactca aggagttcgt catgagcaaa atatcagatt
    26401 tgaattattc tcaacacatt acattagccg acaattttaa acaaaaaagt gaagttttaa
    26461 atacctggcg tgttggaatg aataattttg cccgtaatgc cgaggggcag gataacacaa
    26521 gaaatatcct tgatcctaag acatttttgg aatttttggt aaaaatattt actctaggtt
    26581 atgtggattt tagcaaacgc tccaacgaag cgggaagaaa tatgatggct catattgagt
    26641 cctcatctta tatcaaaaat aatgatggca gtgagataat aaagtttgtt atgaataatc
    26701 ctgaagggga acgagcggat ttatctaagg tggaaataga aatcacactt tccactttca
    26761 ctactatggg gactcgtcaa ggacatacgg ctattatatt tcaacaacct gatggttcga
    26821 ctaatcgtta tgaaggaaag tcctttgaaa gaaaagatga gagttcatta cacctgatta
    26881 ctaacaagat tctggcatgt taccaacgcg aagctaacaa ggaaatagct cgtctattaa
    26941 atatttcaca ggagttaaat aattctcagg acttgaataa ctcgcaggtg agttgtaaag
    27001 attcagttga ttctacgatt acggatttat tagaaaaacc attgaataat gcattattag
    27061 caataaggaa agaacatctg ctattaatgc cttatgtatg taatgaatcg atttcatact
    27121 tgcttggtga aaaagggata ctgaaagaaa tagatgacct taacgcagta aataattatc
    27181 ttcttaataa taagaaagct act
//
LOCUS       DAJTSL010000042        27127 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTSL010000042 DAJTSL010000000
VERSION     DAJTSL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.042"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..148)
                     /gene="fumD"
                     /locus_tag="OO433_001585"
     CDS             complement(<1..148)
                     /gene="fumD"
                     /locus_tag="OO433_001585"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR6050103.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKR"
     gene            705..2117
                     /gene="pykF"
                     /locus_tag="OO433_001586"
     CDS             705..2117
                     /gene="pykF"
                     /locus_tag="OO433_001586"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6050104.1"
                     /translation="MKKTKIVCTIGPKTESEEILAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2428..2664
                     /gene="lpp"
                     /locus_tag="OO433_001587"
     CDS             2428..2664
                     /gene="lpp"
                     /locus_tag="OO433_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6050105.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(2728..3732)
                     /gene="ldtE"
                     /locus_tag="OO433_001588"
     CDS             complement(2728..3732)
                     /gene="ldtE"
                     /locus_tag="OO433_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6050106.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLLPVVPAGPNNPLGRYALRFAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRARQ
                     "
     gene            complement(3881..4297)
                     /gene="sufE"
                     /locus_tag="OO433_001589"
     CDS             complement(3881..4297)
                     /gene="sufE"
                     /locus_tag="OO433_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6050107.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQNQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(4310..5530)
                     /gene="sufS"
                     /locus_tag="OO433_001590"
     CDS             complement(4310..5530)
                     /gene="sufS"
                     /locus_tag="OO433_001590"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6050108.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLHTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSVLGLNNIAEYELNLMHYALSQLESVPNLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(5527..6798)
                     /gene="sufD"
                     /locus_tag="OO433_001591"
     CDS             complement(5527..6798)
                     /gene="sufD"
                     /locus_tag="OO433_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6050109.1"
                     /translation="MAGLPNSSNALQQWHHLFDAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTSLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(6773..7519)
                     /gene="sufC"
                     /locus_tag="OO433_001592"
     CDS             complement(6773..7519)
                     /gene="sufC"
                     /locus_tag="OO433_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6050110.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(7529..9016)
                     /gene="sufB"
                     /locus_tag="OO433_001593"
     CDS             complement(7529..9016)
                     /gene="sufB"
                     /locus_tag="OO433_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6050111.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(9025..9393)
                     /gene="sufA"
                     /locus_tag="OO433_001594"
     CDS             complement(9025..9393)
                     /gene="sufA"
                     /locus_tag="OO433_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6050112.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(9940..10128)
                     /locus_tag="OO433_001595"
     CDS             complement(9940..10128)
                     /locus_tag="OO433_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6050113.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(10228..10638)
                     /gene="menI"
                     /locus_tag="OO433_001596"
     CDS             complement(10228..10638)
                     /gene="menI"
                     /locus_tag="OO433_001596"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6050114.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(10635..13691)
                     /locus_tag="OO433_001597"
     CDS             complement(10635..13691)
                     /locus_tag="OO433_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6050115.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDVLDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPELDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTL
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            14080..15192
                     /gene="ydiK"
                     /locus_tag="OO433_001598"
     CDS             14080..15192
                     /gene="ydiK"
                     /locus_tag="OO433_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6050116.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            15378..15485
                     /gene="rprA"
                     /locus_tag="OO433_001599"
     ncRNA           15378..15485
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO433_001599"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            15621..16000
                     /locus_tag="OO433_001600"
                     /pseudo
     CDS             15621..16000
                     /locus_tag="OO433_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            15945..16667
                     /locus_tag="OO433_001601"
                     /pseudo
     CDS             15945..16667
                     /locus_tag="OO433_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            16679..17545
                     /gene="ydiB"
                     /locus_tag="OO433_001602"
                     /pseudo
     CDS             16679..17545
                     /gene="ydiB"
                     /locus_tag="OO433_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
     gene            17577..18335
                     /gene="aroD"
                     /locus_tag="OO433_001603"
     CDS             17577..18335
                     /gene="aroD"
                     /locus_tag="OO433_001603"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6050117.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            18481..20076
                     /locus_tag="OO433_001604"
     CDS             18481..20076
                     /locus_tag="OO433_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCR6050118.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFNFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIQELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            20090..21241
                     /locus_tag="OO433_001605"
     CDS             20090..21241
                     /locus_tag="OO433_001605"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCR6050119.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFAEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(21284..22195)
                     /locus_tag="OO433_001606"
     CDS             complement(21284..22195)
                     /locus_tag="OO433_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6050120.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLSNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            22509..23280
                     /locus_tag="OO433_001607"
                     /pseudo
     CDS             22509..23280
                     /locus_tag="OO433_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
     gene            23300..24238
                     /locus_tag="OO433_001608"
     CDS             23300..24238
                     /locus_tag="OO433_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6050121.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAAPLKDKHPAMLLLVATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            24294..25583
                     /locus_tag="OO433_001609"
     CDS             24294..25583
                     /locus_tag="OO433_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6050122.1"
                     /translation="MSDDKFDAIVVGADVASSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYSRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            25580..25873
                     /locus_tag="OO433_001610"
     CDS             25580..25873
                     /locus_tag="OO433_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6050123.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            25930..>27127
                     /gene="fadK"
                     /locus_tag="OO433_001611"
     CDS             25930..>27127
                     /gene="fadK"
                     /locus_tag="OO433_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR6050124.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVSVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSPIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDE"
BASE COUNT         6741 a         6893 c         6685 g         6808 t
ORIGIN      
        1 tgcgtttatt ggctaatgct gtacgtaata cgcctgcgat aacaatatgc tttggttcct
       61 gccccagatc gcgcatttcc agcacaactt taccgacaac tctgcacatt tctcggtaca
      121 gttcatcctc ttttgtccga tttcccattc tttataccta tttatcatgc caactatcag
      181 catatatcaa tctaaccaat taaacaaaaa tcaaacaaaa tcagacaaat aacgcgataa
      241 attattttaa ctgtagcaat tgagcgatga tatatttata caccggatga actttcactt
      301 atcctcacac tgacaacttt ggcaccagac gttgcgcaaa cagcgaagtt tttgcgtaac
      361 cttttccctg gaacgttaaa tctttgataa caatttattg tctaacaagt tgtatatttt
      421 ttgaaacgct gtttttgttt tccttttgga ttaatttcag cgtataatgc gcgccaattg
      481 actcttgaat ggtttcagca ctttggactg tagaactcaa cgactcaaaa acaggcactc
      541 acgttgggct gagacacaag cacacattcc tctgcacgct ttttcgatgt cacctatcct
      601 tagagcgagg caccaccact ttcgtaatac cggattcgct ttccggcagt gcgcccagaa
      661 agcaagtttc tcccatcctt ctcaacttaa agactaagac tgtcatgaaa aagaccaaaa
      721 ttgtttgcac catcggaccg aaaaccgaat ctgaagagat attagctaaa atgctggacg
      781 ctggcatgaa cgttatgcgt ctgaacttct ctcatggtga ctatgcagaa cacggtcagc
      841 gcattcagaa tctgcgcaac gtgatgagca aaactggtaa aaccgccgct atcctgcttg
      901 ataccaaagg tccggaaatc cgcaccatga aactggaagg cggtaacgac gtttctctga
      961 aagctggtca gacctttact ttcaccactg ataaatctgt tatcggcaac agcgaaatgg
     1021 ttgcggtaac gtatgaaggt ttcactactg acctgtcagt tggcaacacc gtactggttg
     1081 acgatggtct gatcggtatg gaagttaccg ccattgaagg taacaaagtt atctgtaaag
     1141 tgctgaacaa cggtgacctg ggcgaaaaca aaggtgtgaa cctgcctggc gtttccattg
     1201 ctctgccagc actggctgaa aaagacaaac aggacctgat ctttggttgc gaacaaggcg
     1261 tagactttgt tgctgcttcc tttattcgta agcgttctga cgttatcgaa atccgtgagc
     1321 acctgaaagc gcacggcggc gaaaacatcc acatcatctc caaaatcgaa aaccaggaag
     1381 gcctcaacaa cttcgacgaa atcctcgaag cctctgacgg catcatggtt gcgcgtggcg
     1441 acctgggtgt agaaatcccg gtagaagaag ttatcttcgc ccagaagatg atgatcgaaa
     1501 aatgtatccg tgcacgtaaa gtcgttatca ctgcgaccca gatgctggat tccatgatca
     1561 aaaacccacg cccgactcgc gcagaagccg gtgacgttgc aaacgccatc ctcgacggta
     1621 ctgatgcagt gatgctgtct ggtgaatccg caaaaggtaa atacccgctg gaagcggttt
     1681 ctatcatggc gaccatctgc gaacgtaccg accgcgtgat gaacagccgt ctcgagttca
     1741 acaatgacaa ccgtaaactg cgcattaccg aagcggtatg ccgtggtgcc gttgaaactg
     1801 ctgaaaaact ggatgctccg ctgatcgtgg ttgctactca gggcggtaaa tctgctcgcg
     1861 cagtacgtaa atacttcccg gatgccacca tcctggcact gaccaccaac gaaaaaacgg
     1921 ctcatcagtt ggtactgagc aaaggcgttg tgccgcagct ggttaaagag atcacttcta
     1981 ctgatgattt ctaccgtctg ggtaaagaac tggctctgca gagcggtctg gcacacaaag
     2041 gtgacgttgt agttatggtt tctggtgcac tggtaccgag cggcactact aacaccgcat
     2101 ctgttcacgt cctgtaatat tgcttttgtg aattaatttg tatatcgaag cgccctgatg
     2161 ggcgcttttt ttatttaatc gataaccaga agcaataaaa aatcaaatcg gatttcacta
     2221 tataatctca ctttatctaa gatgaatccg atggaagcat cctgttttct ctcaattttt
     2281 ttatctaaaa cccagcgttc gatgcttctt tgagcgaacg atcaaaaata agtgccttcc
     2341 catcaaaaaa atattctcaa cataaaaaac tttgtgtaat acttgtaacg ctacatggag
     2401 attaactcaa tctagagggt attaataatg aaagctacta aactggtact gggcgcggta
     2461 atcctgggtt ctactctgct ggcaggttgc tccagcaacg ctaaaatcga tcagctgtct
     2521 tctgacgttc agactctgaa cgctaaagtt gaccagctga gcaacgacgt gaacgcaatg
     2581 cgttccgacg ttcaggctgc taaagatgac gcagctcgtg ctaaccagcg tctggacaac
     2641 atggctacta aataccgcaa gtaatagtac ctgtgaagtg aaaaatggcg cacattgtgc
     2701 gccatttttt ttgtctgcta tttaccacta ctgcctcgcg cgtaacatat tcccttgctc
     2761 tggttcacca ttctgcgctg actctactga aggcgcattg ctggctgcgg gagttgctcc
     2821 actgctcacc gaaaccggat accctgcccg acgatacaac gctttatcga ctaacttctg
     2881 atctacagcc ttattgtctt taaattgcgt aaagcctgct ggcagtgtgt atggcattgt
     2941 ctgaacgttc tgctgttctt ctgccgatag tggtcgatgt acttcaacat aacgcatccc
     3001 gttaggctcc acggaatatt tcaccggttc gttgatcact ttcaccggcg ttcccgtccg
     3061 cacgctggag aataaggctt taatatccgg tgcattcatg cgaatacagc ccgaactgac
     3121 gcgtaaaccg acgctgtccg gcgcactggt accatgaatg aggtattcgc cattaccatg
     3181 cgcgaagcgc agtgcgtaac gtcctagcgg gttatttggt ccggcaggaa cgactggcag
     3241 taatttaatg ccacgctcca gcgaacgttg acgaatgcct gccgtaggcg tccaggttgg
     3301 attagggatt ttctgcccca cacgcgtttc catcaccggc gtttccagcc cctgcaagcc
     3361 aatacctatc ggataaacct gcacgatatt ttctcccggc ggataataat aaaggcgcag
     3421 ctctgcaagg ttaacgataa tcccctgacg cggtgcatca ggtaataaca gttgtgaagg
     3481 aatagttatc gtcgtaccag gttttggcac cggggcgata gtgttattgg cttcaaggat
     3541 caacattgcc gcagtatcaa aacgtcgggc aatagcctga aggtttttat ccccttcttg
     3601 caccgtatac gtttgatttt gcccaaccag tcggcttccg gttggtggta gcggataatc
     3661 aaccgcccag gcagcctgga tggcgctaaa agcgccgata agcgtgagtg taagcaaaga
     3721 cgcgcgtttc attgtaaacc tcctgtattt gccggagact cacgctgaaa cgtcggatgg
     3781 cgcttatgtt cacctgaaac caaaacactc ctgtgcaggt cagtgtaaac attgaccatc
     3841 cggcaatgtg agccaaccgg atgaaagctg tccctttagt ttagctaagt gcagcggctt
     3901 tggcgcgaat tgcgcgaatc atcgcttcca gaccttgtga acgagatggg gtgagatgtt
     3961 gggtgagcgc cattttttca aaccacggac gcacatcgaa attgacaata tcctgcggcg
     4021 tcatctgatc gtagagaata aatacgaccg caataagccc tttcacaatc gccgcatcgc
     4081 tgtcgccctg caattcaata attccctgcg cattctggcg catgacaatc cacacctgat
     4141 tctggcagcc ctgaatgcta ttttgtgggc ttcggtcttc gtcgcgtaat tctggcagac
     4201 gctggcccag ctcaataatg tagagatatt tctcttccca gttggcgcag cgtaaaaaat
     4261 tacgcagcaa tttttcttta tccggcaata aagccatagt gcctccctgt tatcccagca
     4321 gacggtgaat acgttgcagg ccggtcacca gacgatccac ttcttcatga gtgttataca
     4381 tggccagcga cgcccgacac atcgcaggga cgttgtaata ggccattaat ggcatagcgc
     4441 agtgatgtcc ggtacgcaca gcaatgccgt aattatcgag aaaactgcca acatcatagg
     4501 cgtggtgttt accgagatta aaagcaataa cgccaagcct gttttgtggg ccatagagag
     4561 tgagattcgg tacagattcc agctgtgata gcgcgtaatg catcagattc agttcatact
     4621 cggctatgtt attaagcccc agcaccgaaa catattccag cgccgcgcca agaccaatga
     4681 tgcccccggt attgggtgta ccggcttcaa accgccatgg tgctttggtc caggtagtgc
     4741 cttcactcag gctgacggtg gcgatcatag aaccgccccc ttcccacggc ggcatctcct
     4801 gcaacaaggc ttctttgaca taaagaatgc caatcccggt ggggccatac agtttatgcc
     4861 cggaaaacac gtaaaaatcg caatccagcg cctgaacatc caccggatga tgcatcaccg
     4921 cctgagcgcc atccaccagc acttttgcgc catgctggtg cgcaagcgtg atcattcccg
     4981 ccagtggatt ttctgtgcca agcacgttgg agacatgagt aattgccagc aggcgagttt
     5041 tctcatcaaa cagcgtatgc agcgtctcca gttgcagcgt accgtcgggg ttgagaggga
     5101 tcacacgcag ctctgcgcca acgcgtgcgc aaagcatctg ccaggggaca atgttagcgt
     5161 ggtgctccat ctgactgatg atgatgttat cgcccgcccg cacattgctg ttgccccagc
     5221 tattggcgac cagattgatc ccttccgtcg tgccgcggac gaacaccagc tcttccgccg
     5281 aacgggcatt aataaacagc gatgcccgct tgcgcacgtt ctccattttc tcggtcgctt
     5341 gggcgcttaa ggtatgaata ccgcgatgca ccgccgcgta gccatgacga taaaactcgg
     5401 cctcggcgtc aatcacctgg ctcggtttct gtgcactggc ggcgctgtcg agataagcca
     5461 gcggcaaacc gtttacctca cgcgaaagca ccggaaagtc ggcccgcact ttgtcgacgg
     5521 aaaaagtcat cttgcacctc ctggcagccg ttgaccgatt cgggccagca cctgctgttt
     5581 aagcccctca tcacgcagtg cttccgtcag ttcggcagca aaggcgtaaa tgatcatctg
     5641 ctgggcatcc tgctgattga tcccgcgcga gcgcagatag aacatctgtt catcatcaat
     5701 acgccccacc gtcgcgccgt ggctgcattt cacatcatct gcatagattt ccagctgcgg
     5761 tttcgtatcc acttccgcca gtttgcccat cagcagatta ttgttggtca tctgaccatc
     5821 cgttttgatg gcgtgctgcg cgacgttgat caaaccgtta aataccgcgc ggcctttgtc
     5881 gctgacgata gttttgtgca actgtcggct gttacaaaaa cctttattgt gttccagcca
     5941 ggtacgggta tcacacacct cgtttttcac cggcatcgcc aggctattga tccgcagcgt
     6001 gctgttttcg ccattgagtt gcgtactggt gttgtgtcgt aacactgcgc cacccagcag
     6061 gaaactatgg ctaaatgcgg tggcatcctc agccagcaac aaatcgttat gagcaaagtg
     6121 gtgactgagc gggttttcaa acgccagctt gatatgctgc aagtgggcgt tcgctgcgac
     6181 attgatagtg aaccgtgccc cggtaaaatg acgagcatca ttaaggctga caaaatgttc
     6241 gatcaccgtt gcttcggcac cttccgccag atccagatga tgccggtaat gggcagtgtt
     6301 cacctcttca cctgccacgc cctgggtgat atacattaac agcaatggct ttgccggccg
     6361 ttgaccgcgc ttcacggcga tatgcgtcac gctttgtgcc aggctttccg tcaaatgcag
     6421 aaacacttcc gcctgaatag cgtcgggtaa cccctgacgg tcgtcgttaa tgctcacttc
     6481 atatccgctg ccttcaattg catcgctcag cgcgggcacg taacgtccat cgacaaacac
     6541 cagccgcacg gcgtctaacg ttaacgctaa ggcatcacgc tgctgtgggg atatctcccc
     6601 cgcaatgctg acaaactggc tattggtcag cccttccagc gacgtatatt tccagttttc
     6661 atgtttacgt gtcggcagtc cggtacgcag caattgttgt aaatgctgct gtgcttgcgg
     6721 ggagcgtttt gtcccttcag cgtcaaacaa gtgatgccac tgttgcagcg cgttactgct
     6781 gttcggtaag ccagccataa ccctgctcct ccagttgttt gaccaacgtg aaatcgccgg
     6841 atttcacaat tcgtccctga tatagcacat ggacgtaatc aggcttgatg tagtcgagaa
     6901 tgcgttggta gtgcgtaaca atgatgaatg agcgcttgcc atcacgcagg gagttcacgc
     6961 catcggcgac cacttttaat gcgtcaatat ccagcccgga gtccgactca tcaagaatgc
     7021 ataactccgg ttccagcacc gccatttgca aaatatcgtt gcgctttttc tcgccgccgg
     7081 aaaaaccaac gtttaccgaa cgggttaata aatcttccgg catcttcagg agagcgattt
     7141 tctcttccat caaatcctga aaatcaaagc ggtcgagcgt ttcctggccg cgatagctgc
     7201 gcactgcatt aagtgccgtt tgcaggaaaa actggttact gacgcctgga atctccaccg
     7261 gatactggaa ggccataaag atgccttcgc ctgcacgatc ttccggcgac agtgcaagca
     7321 aatctttgcc tttgaactca accgtgccgc ccgtcacttc ataatcttcg cgcccggcaa
     7381 gcgttgccga taaggtactt ttgcccgaac cgtttggccc cataatggcg tgaacttcgc
     7441 cgggacgaac ggcgaggctt aatccacgca ggatagcttt atcttccacg ctgacgtgta
     7501 aatctttaat acttaacatg tttattcctt atccgacgct gtgttcaaga ctgatcgcga
     7561 ggagtttttg tgcttcaacg gcaaattcca gcggcagttc tgagaacacg tctttgcaga
     7621 aaccgttaac aatcatcgag atggcgtctt cttcgctgat cccgcgttgc aggcagtaaa
     7681 acagttgatc ttcaccaata cgtgatgtcg ttgcctcgtg ctccagttgt gcgctgttat
     7741 tgcgacattc gacatacggg aaggtatgcg ccccacaatt agcgccaatc agcattgagt
     7801 cgcactgagt gaaattgcgc gcattggttg ccgtcggcat gattttcact aagccgcgat
     7861 aactgttctg actatgtccg gcagagatcc ctttcgagat aatggtcgat ttggtgtttt
     7921 taccgatgtg gatcatcttg gtgccggtat ccgcttgctg atgaccgctg gtcagcgcca
     7981 ctgagtaaaa ctcaccaatg gagttatcgc cgcgcaaaat gcagctggga tatttccacg
     8041 taatcgcgga cccggtttct gattgcgtcc atgacatttt gctgttttcg ccttcgcaca
     8101 aagcacgctt ggtgacgaag ttgagaatac cgccggtgtt gttatcgcca gggaaccagt
     8161 tttgtaccgt ggaatatttc acctcggcgt ttttatggat gatgacttcc accactgccg
     8221 cgtgtaactg atagctgtca cgcaccggag cggaacagcc ttcaatgtag ctgacgtagc
     8281 tgtcttcgtc ggccaccaga atggtgcgct caaactgccc ggttttttcc gcgttaatgc
     8341 gaaaataggt ggaaagttcc atcgggcagc gcacgccttt aggcacataa ataaacgtac
     8401 cgtcagaggc taccgccgca ttaagcgcgg caaagaagtt gtcattcccc ggcaccacgg
     8461 tgccgagata tttacgcacc agttccgggt gatcgtggat cgcctcacca aaggaacaga
     8521 aaataattcc ctgctccgcc agtttttcgc gataagtagt ggcaaccgaa acagagtcga
     8581 aaatggcatc caccgccacc tctttgcctt cccgcacggg aacgcccaac tgctcaaacg
     8641 ccgcctccac ctctttactt aaaaaggcgt tcgcgccagt ttgctgcacc gcgccaggtt
     8701 cagacgcgca agtgtcgtca caattaccgc acgatggtgc tgagtagtag ctgtaatcct
     8761 gataattcag cttgtcgtag tgcgctttca accagtgcgg ttcttccatc tccagccatg
     8821 cgcgataggc gtttagacga aactccagca tccactccgg ctcattacgc ttcgccgaaa
     8881 ttgcgcgcac cacctcttcg tttatcccct ttgccagctc atcggtggct aactgggtga
     8941 agaatccttc tttataattc agcgggccgc cggtccaggt tttgacatcg tcagttgctt
     9001 cagtattacg agacatagta ccgcctatac cccaaagctt tcgccacagc cacattcatt
     9061 ctgggcttta gggttgtgaa atttgaatat ctgattaagt ccttcacgaa cgaaatcgac
     9121 ttccgtgcca tcaataaacg gcatcgcttg cagcgggaca aacagcttcg cgccgtcgtg
     9181 ttcaaacagc agatcgtctt tgtccggctc gctaacactg tcgagcacat agccaaagcc
     9241 cgcgcagccc gtttgcttca cgcctaaacg cacgccgacc atacccggct gctttgccac
     9301 cagctcacgg atgtgtatcg ccgctgcggg tgtcagcgtt aagccttgcc aggcgaaatc
     9361 ttgtgggtta aaggttcctg aatgcatgtc catcgattta cctcacttca tcgctttcag
     9421 cgtataacag catgttggtg ataatgatta tcagttcaac ccagcaaacg caggggcttt
     9481 accgtaaaac atgctttttg cctgctttta ataagcatag accctgatgt gtgggttaac
     9541 aggcacgcta agaattaggt atctcattgt tagataatgg ttattatcta aggagcatta
     9601 aggctgtgat gaaaaagaaa aagttgtatt aaaatgacta tttaagagat aggtaaaaaa
     9661 gtgcagcgtt cagaaataag aaaaccctta agtctgtgcg acacaggctt aagggtttct
     9721 accccatccg gcgcttatct ccggcactct cagtggctta gctcttgaag gggcgataag
     9781 aataatctca taaagctaac ccgccgtttt aacacaaact gcgattagta ttatttttga
     9841 acaatatcag gcggtagata agcagtatta agaaggtcat cgaacctgga cggaggttaa
     9901 tccaggtcga tttggcgaac ttgcggcatt aagtcaggat caatgcacgc ccagacgcca
     9961 ggcaaagtag atttcttctt ttaattcagc agaagagaga gtaagcaggt cagcaaattc
    10021 aagttctagt tgtttcagac gtttgagata ttgggcaggt gaaagattgc tctggtcacg
    10081 gcgtaaaaat tcaatggcca gctgggtggg atcaagttga gtagacatag catcctcgct
    10141 tttagacaag acgtgtacag tataccaccg tttactgtgc agataatgac caaaagcaat
    10201 atgcgtcaca cttttctggt gacaacgtca caaaatggcg gtcgtcaatc gtgacgaaca
    10261 gcacaaacgc cctttctcat cgaagatttc aatctgccag acctggtgac gcgaaccgag
    10321 atgcaacggt ttacatatgc cgcgcacccg cccttctcgt gccgagcgga cgtgattagc
    10381 attgatttcc agaccaacca ctttttgctc accttcggta cataaataac cggcaacgga
    10441 accgatactt tcggccagta ccacggatgc tcctccatgc agcaacccga aaggctgctt
    10501 tgtccgcgag tctactggca ttgtcgcttc aagggtgtca tcaccaatat gttcaaagcg
    10561 aatatccagg aaccccacca tgtttccttc acccatagca ttcagtgctt ccagggtgat
    10621 tttccgtttc catatcattt aataatctcc agtaaagcct gcacaggatg gcgtaccccc
    10681 gtgccttcaa cccgttttac ctggctacgg caggaatatc cggtcgccag acagcggtta
    10741 cgcggcagtc gctgcatagc ctgatgccag gataactcat agatcccgag cgaattttca
    10801 tggtttttcg cttcatgtcc gtaagtccct gccatgccgc agcaacccac gctgacattt
    10861 tccagtttcg cgccaaaacg ggcaaatatc gcggcccatt gtgctggcgc acccggcaag
    10921 gcggtaactt cggtacagtg accaaagaaa taccatgatt caccgctgac tgtagccaca
    10981 ggctgtgact caagtgcgct tgccagccat tcattcgcca gtaagacgtt aaactcgcca
    11041 cgctcctcgc ccagcgccag tttatattca tcgcgataac aaagtaccag cgccggatcg
    11101 acgcccacca ttggcatacc cagcttcgcc atacggttga ggaaatccgc cgtctttttc
    11161 gccgtcttcg caaaacgatt aagaaaacct ttaatatgct gggctttgcc atttggcgaa
    11221 aatggcagta acacaggctg gaaacctaat ttttcgacca gacggacaaa atccgccacc
    11281 acttgcgcat cgtaatagct ggtaaagggg tcctgcacca ccaacactgt gcgcgctttc
    11341 tgctctgcat tgagagattc aagctgttcc agcgtcatgt ttgccgagcg atgccccacc
    11401 atttgttgtt gtagcgaggg gaccgacagc agcggcaaat caaccatgcc gatatgtttt
    11461 tccgagagtt tgcgcaccag cggctggtta atgaagaagt taaaggtttt cggcgcgcgt
    11521 gccatcagcg gcgcgtagct ctcgaccgta gcgacgaggt ggtcgcgcag cgggcgtaaa
    11581 taacgggtgt gatagagctg cagaaaacga gagcgaaact ccggcacatc aattttgatg
    11641 gggcacaggg tcgaacacgc tttacaggcc agacagcccg acatcgcctc tttgacttcg
    11701 tgtgagaagt catattcgcc tttattcgca tgccagctat tgcgcgtgcg ggcaattaac
    11761 gtccgcaaac tgacgccgga ttcaggcagt tcttgttcca gtttgagtgg atcaacgccg
    11821 cgatccgcca acaaacgcag ccattcacgc accagcgttg cgcgcccttt cggtgaatga
    11881 atccggttct gggtgatctt catcgacgga cacatcggac tacgggcatc aaagttgaag
    11941 cataaaccgt tgccgttaca ctccatcgca ccgcgccact gctggcgtac cgcaatgggg
    12001 atctgccgat cgaatgtacc gcgcttcacc gcgtccactt tcatcatcgg cgcatcgaga
    12061 ccttctggcg ggcaaatctt ccctgggttg agtcggttat gcgggtcaaa tgccgctttc
    12121 actttgcgca gttctgcaaa aagttcctca ccgaaaaacg ccgggctgta ttcagcgcga
    12181 aaacctttgc cgtgctcgcc ccacaacaaa ccaccgtatt tcgcagtcag cgccaccacg
    12241 tcatcagaga tttgcttcat caaaatctct tgttgaggat cgcacatatc cagctctgga
    12301 cggacgtgca agacacctgc gtcgacgtga ccgaacatac cgtagcttaa gccgtggctg
    12361 tcgagcagcg cgcgaaattc agcaatataa tccgccaggt gttccggcgg tacgcaggta
    12421 tcctcagcaa acggaattgg cttagcggca cctttggcat tgccaagcag accaacggct
    12481 tttttgcgca ttgcatagat acgttcaacc cccgccagct cacggcacac ctgccagccg
    12541 atgacacctg cttgatgact ggcgatcagc tcatccaacc gcgcacagag tgcatttacc
    12601 cgctcatcaa tcagcgcctc atcatcacca gcaaattcca caatgttcag cccgagcatc
    12661 tcttggtcag gcacatcggt aatcaactcg ctgacggaat gccagacaat atcttcccgc
    12721 gccagattca gcacttttga gtccaccgtc tctaccgaaa gcgcccgcgc ctcaaccata
    12781 aacggcgcgt tacgcagcgc agagtcaaaa gagtcatatt tgacgttcac cagacggcgc
    12841 actttaggca agcgcgtaat atccagccgc gcttcggtaa taaaggccag cgtcccttct
    12901 gaacccgtca gaatgcgcgt caggtcgaac tcggtcatct catcgttaaa gacatgacgc
    12961 agatcgtaac cggtaagaaa gcggttaagt ttggggaagt tgtcgataat taactggcgt
    13021 tgctgacggc aacgttgata aaccgtgtta taaattcgcc cgattgtggt attggattta
    13081 cccagcgttt ccgccaattc gacgggtaaa ggttgcgtat cgagaacatc gccccccaac
    13141 aacaccgcgc gtacgccaag tacgtgatct gacgttttgc catagaccag cgatccctga
    13201 ccggatgcat cggtattgat catcccaccg agcgttgccc ggttgctggt cgaaagttcc
    13261 ggcgcaaaaa agtagccgaa cggtttcagg tactgattga gttgatcttt tatcaccccg
    13321 gcctcaacgc gcacccagcc ctcttcaggg ttaatttcga tgatgcggtt catatggcgg
    13381 gacatatcaa caataatccc ctggttgagc gcctgaccgt tagtgccggt gccgccgccg
    13441 cggggggtaa agatcagcga tgaatagcgt tcctgcgcgg caagacgggc gatcagcgcc
    13501 acatctgcgg ttgaacgcgg aaataccacc gcatcgggga gaagttggta aatactgttg
    13561 tcggtcgaca ttgtcagacg atcggcataa cttgtcgccg tatcgccggt aaaaccttgt
    13621 tgctccagct cttgcaaaaa attaagcacc agttgaacga cgccgggtgc ctgagaaatc
    13681 tgtggaatca ttatattgcc cctttcctgc ggtctgtgat gtaggtcgat acactattct
    13741 ttcaggcggc tgcaatagcg cactgaaagg tgatgtttat ttactctatg gatttcgagt
    13801 tgcaggaagg cggcaagcga gtgaactcca ggagcttaca atagtaagtg actggggtga
    13861 acgaacgtag ccgcagcaca tgcaacttga aatacgacga gtaaatcgtt tgcgtgttgc
    13921 ctgagttgtt gtaccacatt tttttctaac acgcccatca gaattaaggg cagaatcggc
    13981 ctgttaaaaa ccgctgaaat tgctcatcat tatgcaggtg agtttcgcgt gttcacgtcg
    14041 cgtcgacgat ttgacgcaca aaaaaggtga aaagtagtta tggtaaatgt tcgtcagccc
    14101 agggatgtcg cacaaattct gctttcggtg ctgtttttag ccatcatgat tgtggcatgt
    14161 ctgtggattg ttcaaccctt tattctcggc tttgcatggg ccggtacggt ggttatcgcc
    14221 acctggccgg tattgttacg tttgcaaaag atcatgtttg gccgccgctc tctcgccgtt
    14281 ctggtgatga cgctgttatt agtgatggtg tttatcatcc ctattgcttt gctggttaac
    14341 agtatcgtcg acggcagcgg cccgctaatt aaagccattt ccagcggcga catgacgtta
    14401 cccgatctgg cgtggcttaa taccattccg gtgattggcg cgaagctgta tgcaggctgg
    14461 cacaacttgc tggatatggg ggggacggcg atcatggcga aagtccgccc ttatattggc
    14521 accaccacca cctggttcgt tgggcaggcg gcgcatatcg ggcgctttat ggtgcattgt
    14581 gcgctgatgc ttctcttcag tgccctgctg tactggcgcg gtgaacaggt ggcacaaggc
    14641 attcgccatt ttgcaacccg tctggcaggc gttcgcggag atgccgccgt actgctggcg
    14701 gcacaggcta tccgcgcggt ggcgctgggt gtggtggtga cggcgttagt acaggcagtg
    14761 cttggcggta tcggcctcgc cgtatccggc gtaccttatg caacgttgct aacggtgtta
    14821 atgatcctct cctgccttgt ccagcttggc ccgttgccgg tactgattcc ggcgattatc
    14881 tggctctact ggactggcga taccacctgg ggaacggtat tgttagtgtg gagcggtgtg
    14941 gttggcacgc tggataacgt catccgccca atgttaattc gcatgggtgc cgatttaccg
    15001 ctgatcctga tcctctctgg tgttattggt ggtttgattg ctttcgggat gatcggtctg
    15061 tttattggtc cggttctgtt agccgtttcc tggcgtctgt ttgccgcgtg ggtggaagaa
    15121 gtcccgccgc cgacggacca accggaagaa attctcgaag aacttggcga aatcgagaaa
    15181 ccgaataagt aatttctcat caggcggctc tgccgcctga ttgttaaccc ccgctaattg
    15241 atatttgctt gctcttcccc atattttcct gcttaccgcc attcagctgg tagtacctgt
    15301 cgcaaattct ttacagtttt taaactaatg agacgaatct gatcgacgca aaaagtccgt
    15361 atgcctacta ttagctcacg gttataaatc aacatattga tttataagca tggaaatccc
    15421 ctgagtgaaa caacgaattg ctgtgtgtag tctttgccca tctcccacga tgggcttttt
    15481 tttaacattt ttccacttcg ctacctcgcc cctcactctt tccaataatg ttgcacaaga
    15541 tactgtgact ctcttcaagc cgtcagcagg ctgataatgt tatttatatt ttattgttta
    15601 tttcatgcag ccattacacg atgaacgctt atgaactcca ggcactccga catatttttg
    15661 ccatgactat tgatgaatgc gcacctggat tgcccagacc ggtgacagtg aaagctggcg
    15721 tcagtgggaa aatggcaagt gtgccattcc tgatcgtgta gtcgagcaac tgttggctat
    15781 gcgtcagcaa agaaaaaaac atcttcatgc catcatagag aaaataaata accgcatcgg
    15841 caacaacacc atgcgttttt tccccgactt aaccgcattt caacgagtct accctgatgg
    15901 caattttatt gactggaaga tctatcaatc ggtagccgct gaaggtaatg ctgttgaaaa
    15961 gcaaattccc cagccagttg gtggacgcca gcgtagctaa tgaattaccg caaatgaaca
    16021 gcaaaccgtt agtctggctg gaaggtgttt catcggtact gttcggtgta gccgcattct
    16081 cgacctttta tgtgattgtg gtgtggatgc ccaaatatgc gatggctttt gctggtatgt
    16141 cagaagctga ggcattaaaa accatctctt attacagtat gggctcgttg gtctgtgtct
    16201 ttatttttgc cgcactactg aaaaaaatgg tccggcccat ctgggctaat gtatttaact
    16261 ctgcactggc aacaataaca gcagccatta tctatctgta cccttctcca ctggtgtgta
    16321 atgccggcgc ctttgttatc ggtttctcag cagctggcgg cattttacag ctcggtgttt
    16381 cggtcatgtc agagtttttc ccaaaaagca aagccaaagt caccagtatt tatatgatga
    16441 tgggtggact ggctaacttt gttattccgc tgattaccgg ttatctgtcg aacatcggcc
    16501 ttcaatatat cattgttctc gattttactt tcgcgctgct ggccctgatt accgcaatta
    16561 ttgtttttat ccgctattac cgcgttttta ttattcctga gaatgatgtg cggtttggcg
    16621 agcgtaaatt tagcacccgg ttaaacacaa ttaagcatag aggttaaagg agttaattat
    16681 ggatgttacc gcaaaatacg aattgattgg gctgatggcc tatcctatcc gccacagttt
    16741 atcgcctgaa atgcagaata aagccttaga aaaagcggga ttgccattta cctatatggc
    16801 ctttgaagtg gataacgata gttttcccgg agcaattgaa ggattaaaag ctctaaaaat
    16861 gcgcggaact ggtgtatcaa tgccgaacaa acaactggct tgttaatatg ttgatgaatt
    16921 aacgccagca gccaaactgg tgggtgccat caacaccatc gttaatgatg atggctacct
    16981 gcgcggctat aacaccgacg gcacgggcca tattcgcgcc attaaagaga gcggttttga
    17041 tatcaaaggc aaaacaatgg tgctgttagg ggccggaggt gcctcaacgg ctattggcgc
    17101 gcagggggca attgaaggtt taaaagaaat taaactcttt aaccgtcggg atgagttctt
    17161 cgataaagcc ctcgccttcg cgcagcgggt taacgaaaac accgattgtg tcgtcacggt
    17221 taccgatctc gccgatcagc aagcctttgc tgaagccctg gcttccgccg acattttaac
    17281 caatggcaca aaagtgggta tgaaacccct tgagaatgaa tcattagtta atgatatcag
    17341 tctgttacat ccgggacttc tggtcactga atgcgtgtat aacccgcata tgacgaagtt
    17401 attgcagcag gcgcaacaag ctggttgcaa aacgattgat ggatacggca tgttgttgtg
    17461 gcaaggggct gaacagttca cgttatggac tggcaaagat ttccctctgg aatatgttaa
    17521 acaggtcatg gggttcggtg cctgacaggc tgaccgcgtg cagaaagggt aaaaaaatga
    17581 aaaccgtaac tgtaaaagat ctcgtcattg gtacgggcgc acctaaaatc atcgtctcgc
    17641 tgatggcgaa agatatcgcc cgcgtgaaat ccgaagctct cgcctatcgt gaagcggact
    17701 ttgatattct ggaatggcgt gtggaccact ttgccgacct ctccaatgtg gagtctgtca
    17761 tggcggcggc aaaaattctc cgcgaaacca tgccagaaaa accgctgctg tttaccttcc
    17821 gcagtgccaa agaaggcggc gagcaggcga tttccaccga ggcttatatt gctctcaatc
    17881 gtgcagccat cgacagcggc ctggttgata tgatagatct ggagttattt accggcgatg
    17941 atcaggttaa agaaaccgtc gcctgcgccc acgcgcatga tgtgaaagtt gtcatgtcca
    18001 accatgactt ccataaaacg ccggaagccg aagaaatcat tgcccgtctg cgcaaaatgc
    18061 agtccttcga cgccgatatt cctaagattg cgctgatgcc gcaaagtacc agcgatgtgc
    18121 tgacgttgct tgccgcgacc ctggagatgc aggagcagta tgccgatcgt ccaatcatca
    18181 caatgtcaat ggcaaaaact ggcgtaattt ctcgtctggc tggtgaagta tttggctcgg
    18241 cggcaacttt tggtgcggta aaaaaagcct ctgcgccagg gcaaatctcg gtaaatgatt
    18301 tacgcacagt attaactatt ttacatcagg cataagcaat aatatttcgg cgggaatatc
    18361 ctcccccgcc gaactaaaaa aaatatattc aaccgtattt aataaaaata tttcgtgagt
    18421 ctctgtgcgc taaatatcca tctggcgtag ggaaaatcac acctgaatca gaaattaaca
    18481 atgaaacctg taaaaccacc tcgtattaat ggacgagtgc cggtcctgtc ggcacaggaa
    18541 gcggtgaatt atattcccga cgaagcaaca ctttgtgtgt taggcgctgg cggcggtatt
    18601 ctggaagcca cgacgttaat tactgctctt gctgataaat ataaacagac tcaaacgcca
    18661 cgtaatttat cgattattag tccaacaggg cttggcgatc gcgccgaccg tggtattagt
    18721 ccgctggcgc aagaaggtct ggtgaaatgg gcattatgtg gtcactgggg acaatcgccg
    18781 cgtatttctg aactcgcaga acaaaataaa attattgctt ataactatcc acaaggtgta
    18841 cttacacaaa ccttacgcgc cgccgcagcc caccagcctg gtattattag tgatattggc
    18901 atcgggacat ttgtcgatcc acgccagcaa ggcggcaaac tgaatgaagt cacgaaagaa
    18961 gacctgatta aactggtcga gtttgataac aaagaatatc tctattacaa agcgattgcg
    19021 ccagatatcg ccttcattcg cgctaccacc tgcgacagtg aaggctacgc cacttttgaa
    19081 gatgaggtga tgtatctcga cgcattggtt attgcccagg cggtgcacaa taacggcggt
    19141 attgtgatga tgcaggtgca gaaaatggtt aagaaagcca cgctgcatcc taaatctgtc
    19201 cgtattccgg gttatctggt ggatattgtg gtggtcgatc cggatcaaag ccaactgtat
    19261 ggcggtgcgc cggttaaccg ctttatttct ggtgacttca cccttgatga cagcaccaaa
    19321 cttagcctgc ccctaaacca acgtaaatta gttgcgaggc gtgcattatt cgaaatgcgt
    19381 aaaggcgcgg tggggaatgt cggcgtcggt attgctgacg gtattggcct ggtcgcccgg
    19441 gaagaaggtt gtgctgatga ctttattctg acggtagaaa caggtccgat tggcggaatt
    19501 acttcacagg ggatcgcctt tggcgcgaac gtgaataccc gtgccattct ggatatgacg
    19561 tcccagttta atttttatca cggtggtggt ctggatgttt gttatttgag ttttgctgaa
    19621 gtcgaccagc acggtaacgt cggcgtgcat aaattcaatg gcaaaatcat gggtaccggt
    19681 ggatttattg atatcagtgc cacctcgaag aaaattattt tctgcggcac gttaaccgcg
    19741 ggcagtttaa aaacagaaat tgccgatggc aaattaaata tcgtccagga aggacgggtg
    19801 aagaaattta ttcaggaact accggaaatt acttttagcg gaaaaatcgc gctcgaacgg
    19861 gaactggatg ttcgttacat caccgaacgt gctgtattca cgctgaaaga agacggcctg
    19921 catttaatcg aaatcgcccc tggcgtcgat ttacaaaaag atattctcga caaaatggat
    19981 ttcaccccag tgatttcgcc agaactcaaa ctgatggacg aaagattatt tatcgatgcg
    20041 gcgatgggtt ttgtcctgcc tgaagcggct cattaacagg agtatcacga tggatttttc
    20101 tttaactgaa gaacaagaac tgctgctggc cagtattcgc gaactgatta cgactaactt
    20161 tgcggaagag tatttccgca cctgcgatca aaacgggaca tatccgcgtg agtttatgcg
    20221 ggcgctggcg gataacggca tttctatgct tggcgtgccg gaagagttcg gtggcatccc
    20281 tgcggattac gtcacccaga tgctggcgct gatggaagtg tcaaaatgcg gtgctccggc
    20341 atttttgatt accaacggtc aatgtattca cagtatgcgc cgtttcggtt ctgcagagca
    20401 gctacgtaaa acggcagaga gcaccctgga aacgggtgat cccgcctatg ccctggcgtt
    20461 gacggagcca ggcgcaggct cagacaacaa cagtgccact accacttaca cgcgtaaaaa
    20521 cggcaaggtt tacatcaacg gacagaaaac ctttattacc ggtgcgaaag agtacccgta
    20581 tatgctggtg ttggcgcgcg atccgcaacc gaaagatccc aaaaaagcct tcaccctgtg
    20641 gtgggtcgac tccagtaagc ccggcattaa gattaacccg ctgcataaaa tcggctggca
    20701 tatgctcagc acctgcgaag tctatctcga caacgtggag gttgaagaga gcgacatggt
    20761 gggcgaagaa ggaatgggtt tcctcaatgt gatgtacaac tttgagatgg agcgcctgat
    20821 caacgccgcg cgcagcaccg gctttgccga atgcgccttt gaagatgccg cccgctatgc
    20881 caaccaacgt atcgcttttg gtaagcccat tggtcataac cagatgatcc aggaaaaact
    20941 ggcgctgatg gcgattaaga tcgacaacat gcgcaacatg gtgctgaaag tggcatggca
    21001 agccgatcag catcagtcac tgcgcaccag cgcggcgctg gcaaaactgt attgcgcacg
    21061 taccgcaatg gaagtcattg atgatgcgat tcaaatcatg ggcggtctgg gctataccga
    21121 tgaagcgcgc gtctcccgct tctggcgtga tgtccgttgt gaacgtatcg gcggcggtac
    21181 agacgaaatt atgatttacg tagcaggtcg gcagatcctg aaagactatc agaacaaata
    21241 atctgcaggc ggcgcagctt cttaacaaac tgcgccgcca gatttatcca acaagactta
    21301 ccggttgagg aaattcagat aggatttctt gttccgcaaa acagttttta aattgtctac
    21361 ggtaatcgct gggcgaacaa ccgacatggc gcaaaaacag tttggcaaag tgatcgacat
    21421 tttcataacc cacacgccag gagatctctg cctgtgataa ttcagtatta gtgagtgacc
    21481 attttgcttc cgtcatacgc cgttgaataa catagttaat aggggaaata cgatactctt
    21541 tggtaaattc atggcaaata taactgacgc tggcacgaaa ttttttcgat aactgttcta
    21601 aagtgatttt ttcgcgataa ttattactca gatagaaaag aatatctttt atcagaacat
    21661 cttttttaat atacccctgc tccgaacgat aagcattttt aaaattttcg tagtaaagaa
    21721 tggctaacgt ataggcaaat gcatcccata acgaagatgt ttggctattt ttactttgcg
    21781 gcaaaatcac acttagctca ttaaataagg tttttatgac ttcttttccc tgccccgcgg
    21841 caattaccgg acaagaatgc ggttgcagta gctgattttc ctcagccccc tgaaactgaa
    21901 agccgtacag cgcacaggta cacgtcgttg ccggatcgtt aacgtcagag gccaccgcat
    21961 gcagcctgcc gcgttctatc accacaatgt catccgcatg tgcgacatat aacgaagaat
    22021 cgatggtaaa ccttgcaacc cctttcttga cgtaaatcaa ctcggtttca ttgtcatgaa
    22081 cgtgatgtcc ggactcccat tttggatcat cgctaaatgc aaaacgtgaa agccgtggcg
    22141 ttttaccggc gacaaacagc gtttcactgg cgttatcaaa acagcgttga tacatgacaa
    22201 cctccctatt ccatgagcaa gcaaaaacaa tatatgccgg ataaaatacg gcgtgtattg
    22261 cggttataca accgcgttta gcgagagcta aacaagatta tttacactga atgcaagatt
    22321 gtacggtcat ggaataacta tattgccgca aattgcactt ttgttttagc gatcgcattt
    22381 ttttgcaaga ttgttggcaa ggaaaacagc ttgctccgtc gaaaaccccc accgctatcg
    22441 cacactattt tcaggccatt tttaccttcc atcggagatg gttccgtatg cgactcacag
    22501 gagaaatcat gaaaataata acctgcttta agctggtgcc tgaagaacag gacattgttg
    22561 tcactccaga atacaccctg aatttcgaca atgccgacgc caaaatcagc cagttcgatc
    22621 tcaatgccat tgaagctgca agccagctcg caacagatga cgatgagata gccgcgctga
    22681 ccgttggcgg ctcattgttg cagaactcga aagtgcgcag tgcgcaaaga tgtgctatcc
    22741 cgcgggccgc acagcctgta tttggtgcag gatgcgcaac ttgaacatgc actgcctctc
    22801 gataccgcaa aagcgctggc ggcagcaatt gaaaagatcg gcttcgattt actgatcttt
    22861 ggtgaaggtt ccggcgacct ttatgcccag caggttggct tgctggtcgg agaaattctg
    22921 caacttccgg tgattaatgc agtgagtgct attcagcgtc agggtaatac actggtgatt
    22981 gaacgcacgc ttgaagatga tgttgaagtt attgaactct ctgttccagc cgtgctctgc
    23041 gtcacctccg atattaacgt gccacgcatt ccttcgatga aagccattct cggcgcgggt
    23101 aaaaaaccgg taaatcagtg gcaggcaagt gatattgact ggagccagag cgcgccactt
    23161 gctgaactgg tcggcattcg cgtaccgccg caaacagaac gtaagcacat cattatcgat
    23221 aacgattcgc cggaggccat tgccgagctg gcggaacatc tgaagaaagc cctgaactga
    23281 agcctacgga gaagaaacga tgagtcaatt aaacagcgtc tgggtcttta gcgataatcc
    23341 tgaacgttat gctgaactgt ttggcggcgc tcagcaatgg ggccaacagg tgtatgccat
    23401 tgtacaaaat accgaccagg cgcaggcagt tatgccttat ggtccaaaat gtatttatgt
    23461 tcttgagcaa aacgacgcgc tgcaacgcac tgaaaattac gccgaaagca ttgctgcccc
    23521 gctgaaagat aaacaccccg ctatgttgct gttggtcgcg acgaaacgtg gtaaagctct
    23581 ggcagcacgg ttaagtgtgc aactgaatgc ggcgctggtg aacgatgcca cggcggtgga
    23641 tattgtcgat ggtcacattt gcgccgaaca ccggatgtat ggcgggttag cgttcgctca
    23701 ggaaaagatc aacagcccgc tggcgatcat tacccttgca cccggtgttc aggaaccgtg
    23761 caccagtgat acctctcatc agtgcccgac agaaacggta ccttatgttg ctccgcgtca
    23821 tgaaattctc tgtcgcgaac gccgtgcgaa agccgcaagc agcgtggacc tgagcaaagc
    23881 aaaacgtgtg gttggcgtcg gtcgtggtct ggcggcgcag gatgacctga aaatggtcca
    23941 cgaactggcg gcggtgctga atgctgaagt cggctgttca cgtccaattg ccgaaggcga
    24001 gaactggatg gagcgtgaac gttatatcgg tgtctccggc gtgttgctga aatccgatct
    24061 ctacctgacg ctggggatct ccgggcagat ccagcatatg gttggcggca acggcgcaaa
    24121 agtgattgtc gccatcaata aagataaaaa tgcgccaatc ttcaattatg ccgactacgg
    24181 tctggtgggc gatatctaca aagtcgtccc tgccctgatt agccagttga gccgctaatt
    24241 cccctttcaa ccacatctcc cgctgtaaac gcagcgggaa gggagcataa cgcatgtcgg
    24301 atgacaaatt tgatgccatt gtggtcggtg cggacgttgc tagtagcgtt gccgcactgg
    24361 tcatggcgcg agccggactg gatgtcctgg tgatagaacg cggcgacagt gccggatgta
    24421 aaaacatgac cggcgggcgt ctttatgccc acacacttga agcaatcatt ccaggctttg
    24481 cagtatcagc gccggtagaa cgcaaggtca cacgcgagaa aatctccttc ttaaccgaag
    24541 aaagcgccgt taccctcgat tttcaccgcg agcaaccaga tgttccgcaa cacgcatctt
    24601 ataccgtatt gcgtaatcgt ctggacccgt ggttgatgga acaagccgag caggcgggcg
    24661 cacagtttat cccgggcgtt cgcgtcgatg cgttggttcg tgaaggaaac aaggtcactg
    24721 gcgtgcaggc cggggatgat attctccaag cgaatgtggt ggttctagct gatggcgtaa
    24781 actcgatgct tggccgctcg ctgggaatgg ttcccgcttc cgatccgcat cattacgctg
    24841 ttggtgttaa agaggttatt ggcctcacac cagaacagat caacgatcgc tttaatatta
    24901 cgggcgagga aggtgccgcc tggctgtttg ccggctcccc ttctgacggc ctgatgggcg
    24961 ggggatttct ctataccaac aaggattcca tatccttggg gctggtttgt ggattgggtg
    25021 atatcgccca tgcgcaaaaa agcgtgccgc aaatgctgga agattttaaa caacaccccg
    25081 ccattcgccc actaattagc ggcggcaaac tgcttgaata ttccgcgcat atggtgccag
    25141 aaggcggtct ggcaatggtg ccgcagatgg ttaacgatgg cgtgatgatc gttggtgacg
    25201 ccgcaggctt ctgcctgaat ttgggtttta cagttcgtgg catggattta gccattgcat
    25261 cggctcaggc tgccgccaca acggtgatcg ccgccaaaga acgcgaggat ttctccgcca
    25321 gcagtctggc gcaatacaaa cgtgagctgg aacaaagctg cgtcatgcgc gatatgcagc
    25381 atttccgcaa gatcccggcg ctgatggaaa acccgcgcct gtttagccaa tactcacgaa
    25441 tggtcgccga catcatgaac gagatgttca ccattgacgg caaaccaaac cagccggtac
    25501 gcaaaatgat catgggacac gcgaagaaaa ttgggctgat caacttgctg aaagatggca
    25561 ttaagggagc aaccgcgcta tgagccagaa cgctacggtt aacgtcgaca tcaaattagg
    25621 cgtcaataaa ttccatgttg atgagggcca cccgcatatc attctggcgg caaatcccga
    25681 tatcaatgaa ttccgtaaat taatgaaagc ttgccctgcc ggactttata agcaggatga
    25741 cgccggcaac attcatttcg attccgccgg ttgtctggag tgcggcacct gtcgggtgct
    25801 gtgcggcaac actattctcg aacagtggca atatcccgca ggcaccttcg gtattgattt
    25861 tcgctacggc taatcatgca tcccacaggc ccgcatctcg ggcctgatgt tctgtttcga
    25921 gagtccaaaa tgaaagtgac attaacgttc aacgaacaac gtcgtgcggc gtatcgtcag
    25981 caagggttat ggggcgatgc ttcactggcc gattactggc agcagaccgc tcgtgcgatg
    26041 ccagacaaaa ttgccgtggt cgacaatcat ggtgcaacgt acacctatag cgcgctcgat
    26101 cacgccgcga gctgtctggc aaactggatg ttagcgaagg gtattgaatc aggcgatcgc
    26161 atcgcatttc aactgcctgg ctggtgtgaa tttaccgtta tctatcttgc ctgcctgaaa
    26221 atcggtgcgg tttccgtgcc gctgttgcct tcctggcggg aagcagaact ggtgtcggtg
    26281 ctcaataagt gtcaggcaaa aatgttcttt gcaccgacgt tgtttaaaca aacacgtccg
    26341 gtagatttaa tcctgccgct gcaaaatcag cttccacaac tacaacaaat tgtcggcgtg
    26401 gacaaactgg ctcccgccac ctcttccctc tcattaagtc cgattatcgc cgacaatacc
    26461 tcactgacca cggcgataac gacccacggc gatgaattag ctgcggtgct gtttacctcc
    26521 ggaaccgagg gtctgccaaa gggcgtgatg ctaacgcata acaatattct cgccagtgag
    26581 cgggcttatt gcgcgcgact gaatctgacc tggcaggatg tctttatgat gcctgcgcca
    26641 cttggtcacg caacgggctt tctgcatggc gtaacggcac cattcttaat tggcgctcgc
    26701 agcgtgttgt tagatatttt cactcctgat gcgtgtctcg cgctgcttga gcagcagcgt
    26761 tgcacctgta tgctaggcgc aacgccgttt gtctatgatc ttttgaatgt actagagaaa
    26821 caacccgcgg acctttcagc gctgcgtttc tttctttgcg gcggtaccac aatccccaaa
    26881 aaagtggcac gtgaatgcca gcagcgcggc attaaattat taagtgttta tggttccaca
    26941 gaaagttcgc cgcatgcggt ggtgaatctc gatgatcctt tgtcgcgctt tatgcacacc
    27001 gatggttacg ctgccgcagg tgtagagatt aaagtggtcg atgacgcacg caagacctta
    27061 ccgccaggtt gcgaaggtga agaagcctcg cgtggcccca atgtgtttat ggggtatttt
    27121 gatgaac
//
LOCUS       DAJTSL010000043        26796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTSL010000043 DAJTSL010000000
VERSION     DAJTSL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.043"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..316)
                     /gene="rpnB"
                     /locus_tag="OO433_001612"
     CDS             complement(<1..316)
                     /gene="rpnB"
                     /locus_tag="OO433_001612"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6050125.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQN"
     gene            complement(513..1286)
                     /gene="hisP"
                     /locus_tag="OO433_001613"
     CDS             complement(513..1286)
                     /gene="hisP"
                     /locus_tag="OO433_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6050126.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEAHERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(1294..2010)
                     /locus_tag="OO433_001614"
     CDS             complement(1294..2010)
                     /locus_tag="OO433_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6050127.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGIYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVLHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(2007..2693)
                     /gene="hisQ"
                     /locus_tag="OO433_001615"
     CDS             complement(2007..2693)
                     /gene="hisQ"
                     /locus_tag="OO433_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6050128.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFFGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(2783..3565)
                     /gene="hisJ"
                     /locus_tag="OO433_001616"
     CDS             complement(2783..3565)
                     /gene="hisJ"
                     /locus_tag="OO433_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6050129.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(3786..4568)
                     /gene="argT"
                     /locus_tag="OO433_001617"
     CDS             complement(3786..4568)
                     /gene="argT"
                     /locus_tag="OO433_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6050130.1"
                     /translation="MKKSILALSLLVGLSVAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(4834..5403)
                     /gene="ubiX"
                     /locus_tag="OO433_001618"
     CDS             complement(4834..5403)
                     /gene="ubiX"
                     /locus_tag="OO433_001618"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6050131.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAISLPEDLFARWQGA"
     gene            complement(5498..7015)
                     /gene="purF"
                     /locus_tag="OO433_001619"
     CDS             complement(5498..7015)
                     /gene="purF"
                     /locus_tag="OO433_001619"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6050132.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQDYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(7052..7540)
                     /gene="cvpA"
                     /locus_tag="OO433_001620"
     CDS             complement(7052..7540)
                     /gene="cvpA"
                     /locus_tag="OO433_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6050133.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(7799..8461)
                     /gene="dedD"
                     /locus_tag="OO433_001621"
     CDS             complement(7799..8461)
                     /gene="dedD"
                     /locus_tag="OO433_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6050134.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(8451..9719)
                     /gene="folC"
                     /locus_tag="OO433_001622"
     CDS             complement(8451..9719)
                     /gene="folC"
                     /locus_tag="OO433_001622"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6050135.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(9789..10703)
                     /gene="accD"
                     /locus_tag="OO433_001623"
     CDS             complement(9789..10703)
                     /gene="accD"
                     /locus_tag="OO433_001623"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6050136.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVEMGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGSIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(10859..11518)
                     /locus_tag="OO433_001624"
     CDS             complement(10859..11518)
                     /locus_tag="OO433_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6050137.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRCSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGMLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(11601..12413)
                     /gene="truA"
                     /locus_tag="OO433_001625"
     CDS             complement(11601..12413)
                     /gene="truA"
                     /locus_tag="OO433_001625"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6050138.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPDDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(12413..13426)
                     /locus_tag="OO433_001626"
     CDS             complement(12413..13426)
                     /locus_tag="OO433_001626"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6050139.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(13492..14628)
                     /gene="pdxB"
                     /locus_tag="OO433_001627"
     CDS             complement(13492..14628)
                     /gene="pdxB"
                     /locus_tag="OO433_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6050140.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIAVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            14727..15722
                     /gene="flk"
                     /locus_tag="OO433_001628"
     CDS             14727..15722
                     /gene="flk"
                     /locus_tag="OO433_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6050141.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(15719..16897)
                     /locus_tag="OO433_001629"
     CDS             complement(15719..16897)
                     /locus_tag="OO433_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6050142.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPLKFALLVVGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17217..18437)
                     /gene="fabB"
                     /locus_tag="OO433_001630"
     CDS             complement(17217..18437)
                     /gene="fabB"
                     /locus_tag="OO433_001630"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6050143.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            18596..20602
                     /gene="mnmC"
                     /locus_tag="OO433_001631"
     CDS             18596..20602
                     /gene="mnmC"
                     /locus_tag="OO433_001631"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6050144.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQSFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQQDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAC
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(20723..21001)
                     /locus_tag="OO433_001632"
     CDS             complement(20723..21001)
                     /locus_tag="OO433_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6050145.1"
                     /translation="MIAEFESRILALIDGMIDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(21035..21583)
                     /gene="epmC"
                     /locus_tag="OO433_001633"
     CDS             complement(21035..21583)
                     /gene="epmC"
                     /locus_tag="OO433_001633"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6050146.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(21583..22392)
                     /locus_tag="OO433_001634"
     CDS             complement(21583..22392)
                     /locus_tag="OO433_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6050147.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFAGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(22392..23216)
                     /gene="mepA"
                     /locus_tag="OO433_001635"
     CDS             complement(22392..23216)
                     /gene="mepA"
                     /locus_tag="OO433_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6050148.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(23220..24305)
                     /gene="aroC"
                     /locus_tag="OO433_001636"
     CDS             complement(23220..24305)
                     /gene="aroC"
                     /locus_tag="OO433_001636"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6050149.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGILLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(24340..25272)
                     /gene="prmB"
                     /locus_tag="OO433_001637"
     CDS             complement(24340..25272)
                     /gene="prmB"
                     /locus_tag="OO433_001637"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6050150.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            25438..25989
                     /gene="smrB"
                     /locus_tag="OO433_001638"
     CDS             25438..25989
                     /gene="smrB"
                     /locus_tag="OO433_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6050151.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(26062..26775)
                     /locus_tag="OO433_001639"
     CDS             complement(26062..26775)
                     /locus_tag="OO433_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6050152.1"
                     /translation="MATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSR
                     VSMSACPGLEQYDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDD
                     DFPDYIGPTKVSSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMEN
                     GKLCNGNNFDERGVYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDT
                     TSRQPIDSTCRFTYILNMY"
BASE COUNT         6608 a         7327 c         6755 g         6106 t
ORIGIN      
        1 ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccggct
       61 tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt ttcacagacc
      121 acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttccagtt
      181 taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat
      241 cccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag
      301 ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc
      361 acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaagtatt
      421 tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccatgaaaa
      481 tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt aaggaactgt
      541 tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc ttcttctatt
      601 ttcccctgat ggaggaaaat gacatgagta gaaacatggc gagcaaagcc catttcgtga
      661 gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg caacacttcg
      721 cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac ttccggttcc
      781 atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag atgcaccgga
      841 tatttcccct gcgcacgttc gtctatcccg acttttgcca gatacttgac cgcccgctcg
      901 tgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccagc
      961 accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt
     1021 aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg caccagattg
     1081 atcgtctggc cattgaccac gatcgaccct tcactcggtt tttcgaggaa gttaatgcag
     1141 cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac atctccggca
     1201 ttcgcttgca gtgagacccc tttcagcact tcatgttcgc cgtagcgttt gtgcaaatcg
     1261 ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg
     1321 ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa
     1381 atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc
     1441 gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag tagagtgcag
     1501 catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat
     1561 aatgcaacga tacattttaa aagtcgagaa gccataggct ctggcggctt caatttcccc
     1621 atgcagaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag
     1681 cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa ggaattcggt
     1741 tcccttaaca atctcaagcg tgtatatgcc ggaatagaac accagcaact gaacatacag
     1801 cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact
     1861 ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta ccgacaaaat
     1921 aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg
     1981 tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacggagtag
     2041 cggcgctcaa ggaacagcag cacaccattg gaaacagtgg tgaaaaccag gtaaatcacg
     2101 ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag ttgggtggct
     2161 ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag gatcacctgc
     2221 cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgccga
     2281 aacacttgcc cacgagtaaa accgaacgcc gtcgccgcct ctatatgtcc tttcggcact
     2341 gccataaaag caccacggaa cgtttcggta aaataagcac cgtagataaa accgagagtg
     2401 ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc ctccgtcacc
     2461 gtgtttagcg caatctgcaa accgaagaaa atcagcagca tcaacactaa gtccggcacg
     2521 ccacgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc
     2581 ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct gatagccagc
     2641 tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatataa catgctgcct
     2701 gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg
     2761 ggctttcttt caccgatggc gattagccac cataaacatc aaaatcgaag tactttttcg
     2821 ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcttcgc
     2881 gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat
     2941 ctttaacaga cgggccaccg aatttgtaat ctttaccgac aggttgtttg aggaaacctt
     3001 cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat
     3061 aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc caatgttcat
     3121 taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct ttcagcgact
     3181 cgactgtcgg ctgaatgtca gaatttttcg ccaccaccaa acgagaatcg gcagcgtaca
     3241 gtttgtcggt gaaggctatt tcttgctgac gtttttccgt aatggaaagc gatgacatga
     3301 tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcgga ttttcgacaa
     3361 acgtacattg cgtattgatg cgtttgcata attcctttgc cagatcgata tcgaagccaa
     3421 ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc
     3481 ggatgttttg cggaatcgca gcaaatgccg cagttgcgct ggagaaggcc agaaccagag
     3541 agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata
     3601 tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc
     3661 cccgttagat gtattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga
     3721 tagtgcaaca aacctagcgt gcaccatgat gatcattcca tcaggtacag cttcccagcg
     3781 acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt gtcgtaggtg
     3841 ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc agcatcatct
     3901 ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac tgatgagcca
     3961 gcaaaggcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc agcaacttca
     4021 tcttgtaacg cagcatccag acgtcctgca gccagatcgg aatagaccaa atcctggttg
     4081 gcataggcca ccacatccac gcctttgcta cgccaggtct cgttagcgta agcttcctgg
     4141 gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt tggctgaatc
     4201 ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc ggagaaggca
     4261 atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatggcgtc gatttttttc
     4321 gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc
     4381 atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc acctttagca
     4441 tctttcgacg agaacggcgc ataggtggta tcggttccga tacgtaccgt ctccggtagc
     4501 gccgcatagc tggaagccgc tacggagaga ccgactaaca aagacagagc gagaatcgac
     4561 ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg
     4621 tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa cattcaacgt
     4681 taaatatgtt aataagacgt tgcattattg tcctgaagtt gaagatagca ggtatagcgg
     4741 ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt
     4801 gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc gggcaaagag
     4861 atcttcagga aggcttattg caaactggtc aagaacacga ttaaccgtct gatttatcac
     4921 atcatcaagg gattgcgggc gatgataaaa cgccggaaca ggaggcataa tcaccgcacc
     4981 gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg tttcacgcac
     5041 gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc
     5101 atcagtatag ctatggacaa tgccggaaag ggttttgatt gaacagggta aaatcaccat
     5161 ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg catcgtgcgt
     5221 gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg
     5281 gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg
     5341 taataagcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt
     5401 cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg cgggagttgc
     5461 aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta tgcatttcga
     5521 gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt aacgtatcga
     5581 ggaaatcgag gtagtcctga tcaacatctt tggtgacgta gacgccgttg aacaccgagc
     5641 attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga tcgttcagat
     5701 cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatcaact tcgcgaccgt
     5761 gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg aagcgaattt
     5821 ccggggccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa
     5881 tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg tttttatcgc
     5941 ggaactcggc gcggttggcg ttcagtttac ggcgcacgga cttacgacgc agctgctggc
     6001 ccggcatgat gaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggct
     6061 tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttccggg atcgggatca
     6121 ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcacccagt ttcgtgccca
     6181 tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg cgggcaaagt
     6241 atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca
     6301 actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa
     6361 agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta cggttctcgt
     6421 caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg aaagcaacca
     6481 taccgtggcc gataatcatc gccacacagg catatgcgcc gcggattaag cggtttgtgg
     6541 cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag ttgtccagct
     6601 cgctagcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt
     6661 tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa
     6721 gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc
     6781 cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc
     6841 gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg
     6901 catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat
     6961 cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc
     7021 tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc
     7081 tttgcagata atcaaaaaac catctgatga taaaactgaa ctgcgggatc agctgtgatt
     7141 tgctccagtc ttcgcttttc gacaccccgg taaaggagtc gagaaagaag agaatggcgg
     7201 caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga tcggtgcctg
     7261 acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca ccaacgatca
     7321 gggtagcgat aaacagtacc gcgatggcaa ttccatttcg aaccagttcg tcttcaaagc
     7381 ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc
     7441 atgtcaccag cgataacgct tcacgaacaa agccgcggat caggctaacc agagaggaaa
     7501 aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg
     7561 ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt
     7621 ccttcccacg catcaataaa aatggcgctg aaaaaatatt caacgccatc gactttttat
     7681 gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat ccgacctacg
     7741 gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca agactgtatt
     7801 aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc aactcaccca
     7861 gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg gtaattttac
     7921 cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc agcttaccga
     7981 caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca taggctttac
     8041 ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc gcaggtggtg
     8101 cttcaacctt cggtttaggc ggctccaccg gtttcggctt cggtggggcg accggtgcag
     8161 gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc gacggtgctg
     8221 cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc ggcaacgcct
     8281 gggtggcggc tggcatcata tcaggctcat cgcgatcgcc cgcttttgga accagcggga
     8341 tagccgcaaa ctcatcctga taatgttttt tctgcccgtc cagcagccct ggaagtacaa
     8401 tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac ttacttgcca
     8461 ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa agaaccacac
     8521 accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc ctgcgcaacg
     8581 ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt ggcaccgcgc
     8641 ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca ggccagagtt
     8701 ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc gtttttcggt
     8761 agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc gacatcaaaa
     8821 ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc gctggcaatc
     8881 ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc cgccagcgct
     8941 gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt gccgtgagca
     9001 tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc ccgacgttgt
     9061 aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc cggctcaccg
     9121 acaattgccg gttttgcgct gcgaaagatg cctgctttct cgcgaccaat actttcgcga
     9181 tctggcccca gccagtcggt atgatccagc gcaatactgg ttactaccgc gacatcggca
     9241 tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat caccacgtca
     9301 agttgtgcct gtttgaacag ccacaacgcc gacagcgtac cgtactcgaa ataggtcagg
     9361 gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc cgattccggc
     9421 aattcctgac cctgcacacg tacgcgctcg gtataacgca ccagatgcgg cgaactgtag
     9481 acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca ggtggtgcct
     9541 ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcagaac gccaagacga
     9601 gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg caggttttcc
     9661 agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt gataatcatg
     9721 gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg ccctgccctt
     9781 atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac gccttcacgc
     9841 ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct cgccagtttc
     9901 aggcgcattt ccggacgacg gacgatcatg tcgatcgagc ctttctcgat caggaattca
     9961 ctgcgctgga atcctagcgg cagtttttcg cgaacggtct gttcgataac acgcggaccg
    10021 gcaaagccaa ttaacgcttt cggttcagcg atgttgagat cgcccagcat ggcgaaactt
    10081 gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa gccgcgctcc
    10141 tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat cagtgcttcc
    10201 tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc ttccagcgcc
    10261 tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc gcccataaag
    10321 gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt catcaccacc
    10381 agcgcatctt tttcgccggt ttctttctgc gcagaagcca gacggtcttt atacttcttg
    10441 gagtcacgaa acttcagcac atctttcggc tcaagctcgc tacccatctc cacaaggctt
    10501 ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg atggtcacac
    10561 ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg accgcagcta
    10621 tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt aatgttgctt
    10681 ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt cgatgccagt
    10741 tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa tgttgcacat
    10801 taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga gttacttttt
    10861 attttgcggc gcgtgccgca gcgcgtttgt gacgaataaa ttcgaaaacc ccaggcaata
    10921 ttgaaacaaa aataatgcca acaatcatca gcttaaggtt gctctgaatg aaggggagca
    10981 tgccgaagaa ataacccgcg taggtaaaaa tcagtaccca caacagtgcg ccgatcacgt
    11041 tataagcggc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac ggcgcgaacg
    11101 ttctgacgat cggtacaaaa cgggcgagaa taatcgtttt gccgccatgt ctctcgtaaa
    11161 actgatgagt cttatcaaga taactgcaac gaaagatttt cgaatccggg ttactgaaca
    11221 gtttgtcacc aaataaccgt ccaatggtat agttcactgc atcaccgaca atagcggcaa
    11281 tcaacataag caccaccatc atatggacat tgagatcgtt agtttccagc gacgccaatg
    11341 caccagcaac aaacagcagc gaatcaccag gcaggaatgg cgttaccacc agccccgttt
    11401 cgcagaacag aatcaaaaac agaatggcat aaacccagac gccgtactcc gcgaccagct
    11461 ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc aggtccatat
    11521 ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta cagcctgaaa
    11581 tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg cggttttgga
    11641 agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc ttttgccgtt
    11701 gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct ctccggctgg
    11761 ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg atgtacaaag
    11821 gcattcgctt tgatatctac caccacataa ggaccgtgac gcgtgacatt aatatgcata
    11881 acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt gaaatcattc
    11941 tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg ttcgtaaaaa
    12001 tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat gatgtagcga
    12061 taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc tttaacccaa
    12121 cgcacggcga tgtcatcagg taaattcgca tttacgccca aggtccacgc cgcgtctttg
    12181 cgctgcgcgg ttgtttcgaa atgcaccacc tgcccggtac cgtgtacccc tgcgtcagta
    12241 cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc cttttccagc
    12301 ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt actgccgtcg
    12361 tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga cattagtaca
    12421 gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg ccaaagcgaa
    12481 cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca ttacgcacgc
    12541 agccaacaga aagatgcgga gtacccgaag catcccctac ctgagtcggg aattcgttct
    12601 cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct tctgccgcca
    12661 gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc ggtgcctgga
    12721 cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact tcgtcaacga
    12781 tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc atattgaacg
    12841 ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc aatttcgcac
    12901 tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa atcaggctgg
    12961 taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc gccagcagct
    13021 ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg gtcagtacaa
    13081 acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac agtccgctgc
    13141 tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg gtagcttctt
    13201 tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg gcatcctgca
    13261 cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta cgtgccagtg
    13321 cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg gcttcgccca
    13381 cagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg gtttactcca
    13441 gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag attaacgtgc
    13501 cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac tggcgtcatc
    13561 acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc gcagtttatc
    13621 gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc gcacatcata
    13681 caccaaatgc accagccttt tcagcgtcgg ttgatcgagc gggccatgca gcgtaatgcg
    13741 accaaactct ggcgcgggca gtaatgtatc cagcgcaacg tgctgttcat gcccaataaa
    13801 cttgctataa gcttcaaaca cttgcgtagt accgcgtgct ttaccttcca gggtataacc
    13861 tgcgatatgc ggcgtgccga tatccacttt tgtcagcaac tccacgttaa gttccggttc
    13921 gccttcccag acatccagca ctacgcttaa cttctggcct tcattcaggc aggtcagcaa
    13981 cgcagtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc ccggcttcag
    14041 gctacggatc agtttttcat ccgccagatg cagcgttttg tacggcccat ctttgaagag
    14101 tggcgtatgg aaagtcagaa tatccgcatg ctgaactaat tcatccagcg agcggaaatc
    14161 accctcatcc ccacggtcag cgcgaggcgg atcgcaaagc aatgttttaa tccctaacgc
    14221 ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg cccacgatcc ccaccgtacg
    14281 gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagt agtgaggaga aaacatattc
    14341 caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg cctgcttcaa
    14401 ccatgcttca tcgacatggt cggtccccgc agtggcagtg ccaacaaatt taatgggttt
    14461 tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg catccgcgtc
    14521 tgccagttga gcgacggcga ttggacgccc gggaaccgcg gtcacctcac ccaaacggct
    14581 aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt ttgtgttacc
    14641 tgtatgagac gagagttaac cggacaagtg tgccataatc tcgcggccag gcatacttgc
    14701 gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct cctcctgggc
    14761 aaccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct ttatccagtc
    14821 agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg caacagcaaa
    14881 gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat tcaccgctgc
    14941 tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg gccgcgcaaa
    15001 aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt ctggggaata
    15061 atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg ctgaatcagc
    15121 tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag atggttattc
    15181 cgcaaccgca gcagcgcgag gcgaccgacc gtcctttatt accggcggag cacaatgcgc
    15241 taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa cagatctggc
    15301 aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg aaactgttta
    15361 accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat acgccgacgc
    15421 tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg gcggcgttat
    15481 cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct tctgcccagg
    15541 ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat ccgcgtgata
    15601 tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat atggcaacgc
    15661 gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg ctggttcgtt
    15721 aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag atcgccccgg
    15781 caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc ggcccggaga
    15841 cgccaagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg cgaacttgtg
    15901 aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa catccggctc
    15961 cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag agcagcaaca
    16021 agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca aaacggtccg
    16081 gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg gtaagagtaa
    16141 agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc ccgataaccg
    16201 caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc ccgacaacgc
    16261 tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg ccgttacagg
    16321 cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa ccgtaatggc
    16381 tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc gccattccgt
    16441 tccatgacat cactttgcca gagtgttttg cccctacgat gcctaatccc caggtcagag
    16501 cgcctgtcag taactggctt tcaccaaagc caagaatcaa acgcccgacg accaacaggg
    16561 caaatttcaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg ccgccagcca
    16621 gaccacaagc taacatcccc tgaagcgccg aacgttttgc accatattga tcggccagtc
    16681 gcccggcata gccacgcgtc agcaccgtag ccagaaactg aatcccgacg gcaatcccga
    16741 ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc ggcaacggca
    16801 gccctacggt catgtaggtg agaaaaaccg caaaagcgat gcggaagagc gaaaaattgg
    16861 cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga atgcagcgca
    16921 aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacagtgcg ttggcaggat
    16981 ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc tggaactggc
    17041 ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt tagtgccgga
    17101 ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga cctactccga
    17161 ctactccgac tactccgact actccgacta ctccgactac tccgacctac tgcgaattaa
    17221 tctttcagct tgcgcattac cagcgtggcg ttggtgccgc cgaagccgaa gctgttagac
    17281 ataacggtgg tcagttcgcg atcggtcgtt tcggtcacga tgttcagacc tgccgcctgc
    17341 tcgtccagct cttcaatgtt gatgctcggg gcgataaagc cgtgttccag catcagcaga
    17401 gagtagatag cttcctgtac gccagcagcg cccagagagt gaccggtcat ggctttggtt
    17461 gcagaaatcg ccgggctctt atcgccgaac acttcacgga tagctgccag ttctttcacg
    17521 tcgccaaccg gagtcgaagt accatgagag ttcaggtaat cgattggggt atcaacgccg
    17581 tgcatcgcca tcttcatgca gcgtactgcg ccttcgccag acggagcaac catgtctgca
    17641 ccatcagagg ttgcgccgta gccaacgatt tcagcataga tgtgagcacc acgcgccagc
    17701 gcgtgttcca gctcttcaac cactaccata ccgccgccgc cagcgataac gaaaccgtca
    17761 cggtgagcgt cgtaagtacg ggaggctttt tccggggtgt cgttgtattt agtagacagc
    17821 gcacccatcg cgtcgaattc gcaggccatt tcccagcaca gctcttcgcc gccgccagca
    17881 aacacgatgt cctgtttgcc cagttggatc tgctctactg cgttaccgat acagtgtgcg
    17941 gaagtcgcac acgcggagct gatggagtag ttaacgccat gaattttaaa cggcgtggcg
    18001 aggcaggcag aaacgccgga tgccatcgct ttggtgacca catacgggcc aaccgctttc
    18061 aggccgcgcg ggccgcgcat tgcgtcagcg ccgaatacct ggaaacgcgg ggaaccgccg
    18121 ccagaacctg caatcaggcc aacgcgcggg ttattctggt aagcttccgg agaaaggccc
    18181 gcatctgcga ttgcctgctc catagaaagg aatgcataaa tggatgcgtc gctcataaag
    18241 cgcacaactt tgcggtcaat gaggccagtg gtatccagtt ttacgttgcc ccagacgtgg
    18301 ctacgcatgc cggaatcctt aagctcctga gagaaagtga tccctgaacg tccttcacgc
    18361 agagatgcca ggacttcctg ctggttatta ccgatgctgg aaacaatgcc caggccagta
    18421 atcactgcac gtttcattca atacctctgt aagtcgcaca tagagtaagt ttcgaatgca
    18481 caatagcgta cacttgtacg ccgaacaagt ccgatcagcc atttaataga gaaatttgcg
    18541 cagccttaca cacatcgcta agatcgagcc accgcctgta agacgagtaa cttacgtgaa
    18601 acactactcc atacaacctg ccaacctcga atttaatgct gagggtacac ctgtttcccg
    18661 agattttgac gatgtctatt tttccaacga taacgggctg gaagagacgc gttatgtttt
    18721 tctgggaggc aaccaattag aggtacgctt tcctgagcat ccacatcctc tgtttgtggt
    18781 agcagagagc ggcttcggca ccggattaaa cttcctgacg ctatggcagt catttgatca
    18841 gtttcgcgaa gcgcatccgc aagcgcaatt acaacgctta catttcatta gttttgagaa
    18901 atttcccctc acccgtgcgg atttagcctt agcgcatcaa cactggccgg aactggctcc
    18961 gtgggcagaa caacttcagg cgcagtggcc aatgcccttg cccggttgcc atcgtttatt
    19021 gctcgatgaa ggccgcgtga cgctggattt atggtttggc gatattaacg aactgaccag
    19081 ccaacaggac gattcgctaa atcaaaaagt agatgcctgg tttctggacg gctttgcgcc
    19141 agcgaaaaac ccggatatgt ggacgcaaaa tctgtttaac gccatggcaa ggttggcgcg
    19201 tccgggcggc acgctggcga catttacgtc tgccggtttt gtccgccgcg gtttgcagga
    19261 cgccggattc acgatgcaaa aacgtaaggg ctttgggcgc aaacgggaaa tgctttgcgg
    19321 ggtgatggaa cagacattac cgctcccctg ctccgcgccg tggtttaacc gcacgggcag
    19381 cagcaaacgg gaagcggcga ttatcggcgg tggtattgcc tgcgcgttgt tgtcgctggc
    19441 gctattacgg cgcggctggc aggtaacgct ttattgcgcg gatgaggccc ccgcactggg
    19501 tgcttccggc aatcgccagg gggcgctgta tccgttatta agcaaacacg atgaggcgct
    19561 aaaccgcttt ttctctaatg cgtttacttt tgctcgtcgg ttttacgacc aattacccgt
    19621 taaatttgat catgactggt gcggcgtcac gcagttaggc tgggatgaga aaagccagca
    19681 taaaatcgca cagatgttgt caatggattt acccgcagaa ctggctgtag ccgttgaggc
    19741 aaatgcggtt gaacaaatta cgggcgttgc gacaaattgc agcggcatta cttatccgca
    19801 aggtggttgg ctgtgcccag cagaactgac ccgtaatgtg ctggaactgg cgcaacagca
    19861 gggtttgcag atttattatc aatatcagtt acagaattta tcccgtaagg atgactgttg
    19921 gttgttgaat tttgcaggag atcagcaagc aacacacagc gtagtggtac tggcgaacgg
    19981 gcatcaaatc agccgattca gccaaacgtc gactctcccg gtgtattcgg ttgccgggca
    20041 ggtcagccat attccgacaa cgccggaatt ggcagagctg aagcaggtgc tgtgctatga
    20101 cggttatctc acgccacaaa atccggcgaa tcaacatcat tgtattggtg ccagttatca
    20161 tcgcggcagc gaagatacgg cgtacagtga ggacgatcag cagcagaatc gccagcggtt
    20221 gattgattgt ttcccgcagg cacagtgggc aaaagaggtt gatgtcagtg ataaagaggc
    20281 gcgctgcggt gtgcgttgtg ccacccgcga tcatctgcca atggtaggca atgttcccga
    20341 ttatgaggca acactcgtgg aatatgcgtc gttggcggag cagaaagatg aggcggtaag
    20401 cgcgccggtt tttgacgatc tctttatgtt tgcggcttta ggttctcgcg gtttgtgttc
    20461 tgccccgctg tgtgccgaga ttctggcggc gcagatgagc gacgaaccga ttccgatgga
    20521 tgccagtacg ctggcggcgt taaacccgaa tcggttatgg gtgcggaaat tgttgaaggg
    20581 taaagcggtt aaggcggggt aatctgctct cgcagtgttt gccggatgcg gcgtgaacgc
    20641 cttatccgac ctacgtgtga cgtgtaggcc tgataagacg cgataagcgt cgcatcaggc
    20701 atggtgctcc agacgccgca acttactgct gtgacgcctg ctgaaacagg ttttcccaca
    20761 tatcggtcac cagcgcctgg tcacgcggcg acaattcacc ggcaccaatg gctttttcca
    20821 gactctggct aacggtcgta tgcaccgcct gagcggagtg gtcatcacca ctttccagtt
    20881 ctgcgatggc taatgtcagg tggccacgca aatacccact ggcaaacaac tcatcatcac
    20941 tggcatggtc aatcatgccg tcgattaatg ccagaatgcg tgattcaaac tccgcgatca
    21001 tcttctttcc tcattgtaaa acgtggcacc ggcttcagtt gagcgcttcc ggccacggga
    21061 actgttccgc cgtaagttcc ggcgtgtgat aataattttg tagtgcttta atgaagcgtg
    21121 ccggacgctc gggaatgcca tttgtcagat aatccatcac ctgcgcatgt acccgccgtt
    21181 gaaacaccac acgatccggt tcgaagtcgc cttccagatt gtcgcaactg acgttaaacg
    21241 gatatcccgc cgccacacag aacaaccagt cgagtgcctg cggcttcact tcaacatctt
    21301 caaactggct ttgcgtttgg gcatcacgcc catccgggca ataccagtag ccgaaatcaa
    21361 ccagctcacg gcgcgctttt ccggcaatac accagtgcga aatctcatga attgcgctgg
    21421 cataaaagcc gtgggcaaag acaattcggt tatacggtac ttccgcatca gcaggaagat
    21481 agatcggttc gtcgtcgcct ttaatcagac gggtattaaa ttcatcggca aagcagctat
    21541 taaaaatttc aatcaactgc tcatagtggt gtgtactgtt cattagttca tccccaacca
    21601 gtggaggatc tcctgtccgt ggctatcgta aagtaatttg gcactcatca ccgccgagac
    21661 gataacgatc atcgggcgga tcagtttttg tcctttgctc agcaccagtc ttgaacccat
    21721 gcgcgcgccg aggaactgcc ccaccagcat gacaaagccc gtcgcccaaa tcactttgcc
    21781 gccgagaata aacagcagca gaccgccaat gtttgacgtt gcgttgagta atttggcgtg
    21841 agccgtggct ttggcgaggt tgaatccgca cagcgtaacg aaggccagcg cgtaaaacga
    21901 tccggcagcc gggccaaaga atccgtcata aaaaccgacg cagccaccgg caattaacgc
    21961 gaagggtaaa ccgtacatcc ggcgctggcg atcttcttca cccagctttg gcatcagcaa
    22021 gaaatagagg ccgatacaaa ttaccagaat gggcaaaatc tgccgtaaga catcagcctg
    22081 aacgtattgc accagcagtg cgccgctcat tgagccggca aaggtcatgg cgatattgag
    22141 tttctgatcg cttaaactaa ccactttgcg gcgaataaag tagatagtgg cggaaataga
    22201 gccgccgcag gcttgcagtt tattggttgc cagcgcgtta gcgggagaca ttcccgcagc
    22261 catcaatgcc ggaatggtga gtaacccacc gccaccggca atcgagtcga taaatcccgc
    22321 cagcatggcg acaaaaaaga gaactcccag caacagcggg gaaaccataa acaggctatt
    22381 aaacgtttcc attagatcac gtgctcatcc agtagcgcct ggcaggaagg cggcaacgga
    22441 ggcggtgtct tcttctcagg ctttgttgtt cctggttttg gaggtgcaaa ccagctttgc
    22501 agttctgccc cgcaaccatc gcctggtggc ggtaaaggtt gatcttcaca ctccagacta
    22561 tcggcagggc aacgtaatcg tacatgcata tgcgcgcgat gctggaacca gggtcgcact
    22621 ttgcgcaacc agtcgcgatc ggtgcccgca tcaaggcaaa gttgttgttt aatcgccgga
    22681 ttaacaaaaa tgcgcgtgac gtctttgtcc tgggcggcga gtttgatcaa gctgaaaatt
    22741 tctggcttcc acagcgtgga gacaacgtgt ttaccgtcgc gggaaactaa gtccagtgct
    22801 tgcgggcgca agagctgcgc ggaggtccag cgagttttcg gcagttgcag aaagatatcg
    22861 acatccagtc cggtctggtg gctggcatga ccgccgttga aacgcccacc agcgggcatc
    22921 cccatatcgc caatcagcac cgtacccatg cccagattgc tcacctggct actcagacgc
    22981 tggataaaca tcaccagatc cgggtgaccg aaatagcgac gctgatcggt acgcatgacc
    23041 tgataatgtt cggactgtat cggcaacgtg tcagcgccga caatacagcc attagaaaaa
    23101 ctgcctatcg attgtgcgct acccggcaca ggttgggtta ttttttgcca cggcgtcgct
    23161 gccaagctag cgctactggc aagcagagcc agcagcgcaa tcgcggtttt gttcattttt
    23221 taccagcgtg gaatatcagt cttcacatcg gcattttgcg cccgctgccg taacaggtga
    23281 tccattaaaa cgatcgccag catcgcttca gcgatcggca ctgcacggat cccgacacag
    23341 ggatcgtgac gacctttggt gatcatctca acttcttcgc caaagcggtt aatagtgcgc
    23401 cccggcacgg taatgctgga ggtcggtttc agcgccatat gagcaatgat ttgctgcccg
    23461 ctgctgatac cgccgagaat gccgcccgca tggttgctct ggaaaccgtc tttggtgatt
    23521 tcgtcgcggt tctggctgcc gcgcagcgcc actacgtcaa agccgtcgcc aatttccacg
    23581 cctttcaccg cgttgatgct catcagcgca tgggcgatgt cggcatccag gcggtcaaat
    23641 accggctcgc caagtccggc ggggacgcca ctggcaacaa cggtgacttt cgcgccgatg
    23701 gagtcgccct cttttttcag cgcgcgcatc agttcatcta acgcgtcgat tttgtccgga
    23761 tccgggcaga agaacggatt ttgttcaacc agcgaccagt ctttgatttc cagcggaatg
    23821 tcgcccatct gggtcaagca gccgcggatt tcaataccaa atttctcggc gagatatttt
    23881 ttggcaatcg cccctgccgc cacgcgcatg gcggtttcgc gggcggaaga acgtccaccg
    23941 ccgcgataat cgcgcagacc gtatttttgt tcgtaggtgt aatcagcatg gcctgggcgg
    24001 aaaacgtctt taattgcgct gtaatcctga gagcgctggt cagtgttttc gatcaacaag
    24061 ccaatgctgg tgccagtagt aacgccttca aaaacaccag agagaatttt gacctgatcc
    24121 ggctcgcggc gctgggtggt atagcgcgat gtcccagggc gacgacggtc gaggtcatgt
    24181 tgcaggtccg cttccgtcag cagaatgcct ggcggaacac catcgacgat gcagccgagc
    24241 gccagcccat gcgattcgcc gaaggtggtt acgcgaaaga gttgtccaat tgtgtttcca
    24301 gccatcacgg ctccgttatt gttgtgtttg cgtgtttact taatctttat aaatcgcgaa
    24361 atattcacgg gccgccagca gctgctcttt ggtgagcata aacacaccat cgccgccgtt
    24421 atcaaactcc agccaggtga acggaacatc cggatattgt tccataagat gtaccatgct
    24481 gttgccgact tcacaaatca acacgccatc atcagcaagg taatctgccg cgtttccaag
    24541 aatgcgacgc gtcagtttaa ggccgtcggt gccagatgcc aggcccagtt ccggctcgtg
    24601 acggtattcg tttggcaggt cggacatatc ttccgcgtcg acatacggcg ggttagtgac
    24661 aatcagatcg tactgcactt tcggcaagtc gcggaacaga tcggaacgaa tcggaatgac
    24721 gttgtggatc agaccgtgtt cttcgatgtt ctgttcagca accgccagcg cgtcgggaga
    24781 gatatccacc gcgtcgactt ctgcatccgg gaaggcataa gcacaggcaa tggcgatgca
    24841 gccgctaccg gtacacatat ctaaaatatg ctgcggttgt ttgctgatga gtccggcaaa
    24901 tttattgttg atcagttcac caatcggcga gcgcggcacc agcacgcgtt catcgacgta
    24961 aaattcatga ccgcagaacc aggctttgtt ggtcaggtaa gccaccggaa tgcgctcgtt
    25021 gacgcggcgg atcacgcgtt caacaatacg atgtttttcg ctggaggtca gacgcgcggt
    25081 gcgcatatct tccgggatat ccagcggcag gtagagcgat ggtaacacca gctgtacggc
    25141 ttcatcccac gggttatcgg tgccgtgacc gtaccagata tttgccgcgc tgaagcggct
    25201 cactgaccag cgcaacatgt cctgaatggt ttgcagctca tttactgctt catcaacgaa
    25261 aattttatcc acgtattcct ccagggcatg atcgcaataa tttcggcggc tagtttgcca
    25321 tgaagatgac gataaatcag cattcacgcg cggtgagtga ggaaaaatac gtttaaaacg
    25381 atcgattgtg ctacgagtcg ggtaaactgt aggaaaatta gaaatagaga cagataaatg
    25441 aaaaagaaaa caacacttag cgaggaggac caggctctgt ttcgccagtt gatggcgggg
    25501 actcgcaaga ttaagcagga cacgattgtc caccgaccgc agcgtaaaaa aatcagcgaa
    25561 gtgccggtga aacgcttgat ccaggagcag gctgatgcca gccattattt ctccgatgag
    25621 tttcagccgt tattaaatac cgaaggtccg gtgaaatatg ttcgcccgga tgtcagccat
    25681 ttcgaggcga agaaactgcg ccgcggcgat tattcaccgg aattgttttt ggatttacac
    25741 ggtctgacgc agctgcaggc caagcaggaa ctgggcgcgt tgattgccgc ctgtcgccgc
    25801 gaacatgtgt tttgtgcctg cgtgatgcat gggcacggga agcatatttt gaagcaacaa
    25861 acaccgctgt ggctggcaca acatccgcat gtgatggcat ttcaccaggc accgaaagag
    25921 tacggcggtg atgcggcttt gttggtgttg attgaagtgg aagagtggtt gccgccggag
    25981 ttgccctgag gagttgagcg tgttgtgcca ggagcgcgga atggcagggg cgaatgaccc
    26041 tgccaggtga gaaaaggtgc gctaatacat attcagtatg taagtgaatc ggcaggtaga
    26101 atctatgggc tgtcgagatg tcgtgtccac gcgtagcttc atatcatgca gttgcctgga
    26161 cgtaatcggt tgcggctcag gcgtcaccgt cactttggca ttatcacagc cggaagtcga
    26221 gaaggtcatc tgctgtgcga taaaacggca gtagacacca cgctcatcaa aattatttcc
    26281 gttacacagt ttgccatttt ccatcaggaa agtgggcagt gtttgctcaa taacaccacc
    26341 tgttgactgc aacctgacga ctttgttatg tacaacataa ttttcattcc aggaaacatc
    26401 atacggtgcc aggggcaccg ccggacatga agaactcacc ttcgttgggc caatatagtc
    26461 aggaaaatca tcatcatacg ttttagcgaa gggatctcta ctcttaacaa acgacgacac
    26521 aatccacgga caaccatgta aattcccatc gtaataaaca aacatcttta attcattaca
    26581 tgtccatccc gttttggtct ggctatcata ctgctctaat ccaggacagg ctgacatgga
    26641 taccctgctg tccgaaattt tcgacataga tggttgagta gaactatcgc cagtccacga
    26701 aactaaatcc ggctgtgaat tgggcatgaa tgtagcctta aatatagcgt aattaactga
    26761 atctggcgtc gctataatca gatgaaactc aaactc
//
LOCUS       DAJTSL010000044        25499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTSL010000044 DAJTSL010000000
VERSION     DAJTSL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.044"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..173
                     /locus_tag="OO433_001640"
     CDS             <1..173
                     /locus_tag="OO433_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCR6050153.1"
                     /translation="ITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPDIGRQ
                     LSPDAWDDVSQE"
     gene            243..1019
                     /gene="yaaA"
                     /locus_tag="OO433_001641"
     CDS             243..1019
                     /gene="yaaA"
                     /locus_tag="OO433_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6050154.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1172..1468)
                     /locus_tag="OO433_001642"
     CDS             complement(1172..1468)
                     /locus_tag="OO433_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6050155.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGNRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(1682..2968)
                     /gene="thrC"
                     /locus_tag="OO433_001643"
     CDS             complement(1682..2968)
                     /gene="thrC"
                     /locus_tag="OO433_001643"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6050156.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLCLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(2969..3901)
                     /gene="thrB"
                     /locus_tag="OO433_001644"
     CDS             complement(2969..3901)
                     /gene="thrB"
                     /locus_tag="OO433_001644"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6050157.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(3903..6365)
                     /gene="thrA"
                     /locus_tag="OO433_001645"
     CDS             complement(3903..6365)
                     /gene="thrA"
                     /locus_tag="OO433_001645"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6050158.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFVQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTSGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6390..6509)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6445..6510)
                     /gene="thrL"
                     /locus_tag="OO433_001646"
     CDS             complement(6445..6510)
                     /gene="thrL"
                     /locus_tag="OO433_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6050159.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(6724..7410)
                     /locus_tag="OO433_001647"
     CDS             complement(6724..7410)
                     /locus_tag="OO433_001647"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6050160.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(7810..7950)
                     /locus_tag="OO433_001648"
     CDS             complement(7810..7950)
                     /locus_tag="OO433_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050161.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8046..8762
                     /gene="arcA"
                     /locus_tag="OO433_001649"
     CDS             8046..8762
                     /gene="arcA"
                     /locus_tag="OO433_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6050162.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(8822..10174)
                     /gene="creD"
                     /locus_tag="OO433_001650"
     CDS             complement(8822..10174)
                     /gene="creD"
                     /locus_tag="OO433_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6050163.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMPLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDWATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(10232..11656)
                     /gene="creC"
                     /locus_tag="OO433_001651"
     CDS             complement(10232..11656)
                     /gene="creC"
                     /locus_tag="OO433_001651"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6050164.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(11656..12345)
                     /gene="creB"
                     /locus_tag="OO433_001652"
     CDS             complement(11656..12345)
                     /gene="creB"
                     /locus_tag="OO433_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6050165.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCSQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLHAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12358..12831)
                     /gene="creA"
                     /locus_tag="OO433_001653"
     CDS             complement(12358..12831)
                     /gene="creA"
                     /locus_tag="OO433_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6050166.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13042..13911
                     /gene="robA"
                     /locus_tag="OO433_001654"
     CDS             13042..13911
                     /gene="robA"
                     /locus_tag="OO433_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6050167.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(13908..14555)
                     /gene="gpmB"
                     /locus_tag="OO433_001655"
     CDS             complement(13908..14555)
                     /gene="gpmB"
                     /locus_tag="OO433_001655"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6050168.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAARRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            14607..15128
                     /gene="yjjX"
                     /locus_tag="OO433_001656"
     CDS             14607..15128
                     /gene="yjjX"
                     /locus_tag="OO433_001656"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR6050169.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15213..15539)
                     /gene="trpR"
                     /locus_tag="OO433_001657"
     CDS             complement(15213..15539)
                     /gene="trpR"
                     /locus_tag="OO433_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6050170.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(15629..17566)
                     /gene="sltY"
                     /locus_tag="OO433_001658"
     CDS             complement(15629..17566)
                     /gene="sltY"
                     /locus_tag="OO433_001658"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6050171.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            17777..19444
                     /gene="ettA"
                     /locus_tag="OO433_001659"
     CDS             17777..19444
                     /gene="ettA"
                     /locus_tag="OO433_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6050172.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(19751..20983)
                     /gene="nadR"
                     /locus_tag="OO433_001660"
     CDS             complement(19751..20983)
                     /gene="nadR"
                     /locus_tag="OO433_001660"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6050173.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21004..22386)
                     /gene="radA"
                     /locus_tag="OO433_001661"
     CDS             complement(21004..22386)
                     /gene="radA"
                     /locus_tag="OO433_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6050174.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22435..23403)
                     /gene="serB"
                     /locus_tag="OO433_001662"
     CDS             complement(22435..23403)
                     /gene="serB"
                     /locus_tag="OO433_001662"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6050175.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            23509..24153
                     /locus_tag="OO433_001663"
     CDS             23509..24153
                     /locus_tag="OO433_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6050176.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24181..25197
                     /gene="lplA"
                     /locus_tag="OO433_001664"
     CDS             24181..25197
                     /gene="lplA"
                     /locus_tag="OO433_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR6050177.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            25229..25378
                     /locus_tag="OO433_001665"
     CDS             25229..25378
                     /locus_tag="OO433_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050178.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
BASE COUNT         6133 a         7026 c         6329 g         6011 t
ORIGIN      
        1 ctattaccaa tttgaccgcc attttactgc tctcgcctgt ggttcatacc attgccagtg
       61 attatctacg ccagcgtaaa ctcggcgtgc gcccggtgtt tgatccgttg cgttatccgg
      121 atatcggtcg ccagctttct ccggacgcgt gggatgatgt ttcgcaggag taatcacaac
      181 tatcgatcaa ctcattctct ttttttgcta aagtcggcat aaatttcctg caaggactgg
      241 atatgctgat tcttatttca cctgcgaaaa cgcttgatta ccaaagcccg ttgaccacca
      301 cgcgctatac gctgccggag ctgttagaca attcccagca gttgatccat gaagcgcgga
      361 aactgacgcc tccgcagatt agcacgctga tgcgcatcag cgacaaactg gcgggtatca
      421 acgccgctcg ctttcatgac tggcagccag atttcacgcc ggcgaatgcc cgccaggcga
      481 ttctggcgtt taaaggtgat gtctacaccg gcttgcaggc cgaaaccttc agcgaagacg
      541 atttcgattt tgcccaacag catttgcgaa tgctttccgg cttgtatggc gtactgcgcc
      601 cgctcgattt aatgcagcct tatcgtctgg aaatggggat ccgtcttgag aatgcccgag
      661 ggaaagatct gtatcaattc tggggagata tcatcaccaa caagctgaac gaggcgctcg
      721 cagcacaagg cgataatgtg gtgattaatc tggcgtcaga tgagtatttt aaatcggtga
      781 agccgaagaa attgaatgcc gagattatca agccggtgtt cctcgatgag aagaacggca
      841 aatttaagat catcagcttc tacgctaaga aagcacgcgg tctgatgagt cgtttcatta
      901 ttgaaaatcg gctgaccaaa ccagagcaac tgactggttt taatagcgaa ggttacttct
      961 ttgatgaagc ttcctccagc aatggcgaac tggtgtttaa acgctacgag cagcgttaag
     1021 tttgcagtca atgccggatg cggcgtgaac gccttatccg gcctacaaaa gcatgcagat
     1081 tcaatatatt gcagaattca cgtaggcctg ataagacgcg ccagcgtcgc atcaggcgct
     1141 gaatgccgga ttgttacccg gcatttgtca tttagcggtg atgtttgcct ggaccatgac
     1201 cgccgtgatg atcatgagga gctttcttat ggtggcgcgg cggtggcggc ggtccgtgtg
     1261 ggtgccagcg attgcctcgc cattcataat gttgtttcca ccagccgtgg tcgcgccagt
     1321 gacctccatc ccagtaatag ccacgattat cacgattgcc tatctgtaat tttactgacg
     1381 ggactaacgt aatttccgca gcctgtgctg ccatgggagc gaccagaacc agggaaagtg
     1441 cgagtacgat agattgcatc tttttcacag gttactcctt ttatcattgc ccgttatggg
     1501 tcgatatccg gagattacgc gatgagaacg acggttgtta ttctccgcaa tcctaatctc
     1561 taagttaccg catttattga gaatatctcc tttttccctg tcgtttttct ccataatttc
     1621 ttcataaaaa agccgggctg cataaaagca aacccggcct gattgagata atgaatagat
     1681 tttactgatg attcatcatc aatttacgca acgcagcaaa atcggcgggc agattatgtg
     1741 aaagcaaggg taaatcagca cgttctgcca gctcttttgg cagatccaac gtttcaccga
     1801 gaatcgcttc cacgctctct ttaaatttcg ccggatgcgc ggtgccgagg aacaagccat
     1861 attcgcctgg attcaactgg tcacgcagcg cacgatacgc tacggcagcg tgcggctccg
     1921 aggtgtagcc cagttctttt aactcacgca ttgtctgttg cgtggtttca tcatccacgg
     1981 ctgcataacc cagctctttc agttgccaga ttttgcggcg gaacaactct tccacacgcg
     2041 gccagttgtt cggctgactc acgtccatcg cgttggataa cgtcgcctga gtcgctttgg
     2101 gtgaccactg accgtcgtgc aggaaacgtg gcacggtatc gttcacgttg gtcgcagcaa
     2161 taaaacgttt caccggcaga cagagtgact tcgccagcag acccgccgtc aaatcgccga
     2221 agtttccgct tggcaccgag acaaccagct ggttgcgcgc ttcctgcggc agctgcgcaa
     2281 ctgcttcaaa gtagtagcaa atctgcgcca gcaaacggct gatgttaatc gagttagctg
     2341 agtttaaccc cagcgccact ttcagctctt catcatcaaa cgcctgcttc accagcgcct
     2401 gacaggcatc gaaatcgccg tcgatggcaa cagtttcgat attgccgccc aatgtacaga
     2461 acagtttttc ttgcagtgga ctgattttgc ctcgtggata gaggataacc actttcacat
     2521 tcggtaaacc gtagaaagca tgagccactg ccgctccggt atcaccggag gtcgcggtca
     2581 gaatggtcac tggcttatcg cccgcaatat gggtcagcat ttgtgccata aagcgaccgc
     2641 cgaaatcttt aaatgccagc gttggcccgt ggaacaattc cagacaaccg acatcgcttt
     2701 caacattggc gaccggagcc gggaaggcaa acgccgcgcg cacgcgctct tccaggattt
     2761 cctgcgggat ttcatcacca ataaacgccg agaggatctt cgcactgcgg gtgacaaaat
     2821 ccagcttcag catctcatca atttcagtca ggctgaattc cggcaggtcg tgcggaaaaa
     2881 acagcccctg atttttgccc aacccctggg ttacggcttg cgcaaagctg acctgctcat
     2941 tgtgatcttt cagattgtag agtttcattt agttttccag tactcgtgcg cccgccgtat
     3001 ccagccggca aatatgaaca aaaccttcct gattttgcag atagttctta cccaaccagt
     3061 cggcaacgcg ctgggcggta tccggcttgt cacacagagc gaacaaggtc gggccggagc
     3121 cggagatacc gctcgctacc gcgccgattt ccgcaaccgc ctgccgcgcc tgccggaagc
     3181 caggcagtaa ccgttcacgg tagggttcag cgataacatc tttcatcagc ttcgcggcaa
     3241 gctcaggctg acgggaatag caggcgtgaa tgaagccagc cagatgtcgc ccgtgcgcaa
     3301 tgcaatcctg gcggcgatac tgcgccggta aaatagccct ggcttctgcc gttgagactt
     3361 taatccccgg atacgccagc acccacagcc actcatcaaa ccctggcact tgctggctga
     3421 tgatgtcgtt ttcttcgatc atcaactgca taccaccaag aaaacacggt gccacgttgt
     3481 cgtaatgaat gctgccggag attcgtcctt ccagctcgcc catcaaagcc agcaaacgag
     3541 tgtcattaag cggcttgcca cagtgttcat tcatcgccat cagcgccgcg accaccgaac
     3601 aggcgctgga gcctaagccc gaaccgattg gcatattctt ttccagagtc atcgccactg
     3661 gaatttgctt gccaagctcc tggcaaaaac gctcccagca ctgataaacg atattttccc
     3721 gtggttctga cggcagctta tcggcaaagc gtccgaggtt gttgagactg aatgtctctg
     3781 ccgcctcaac cgtgactaca tctccgagca atgcaccatc aacaggtgtc accgccgccc
     3841 cgagcacatc aaacccgacg ctcatattgg cactggaagc cggggcataa actttaacca
     3901 tgtcagactc ctaacttcca tgagagggta cgtagcagat cggcaaagac accggcagct
     3961 gtaacgtcat tgcccgcacc atatccgcgc agcaccaacg gcagcggctg ataatagtgg
     4021 ctataaaagg ccagggcgtt ttcgccattt ttcactttga acagcggatc attaccatcc
     4081 acttcggcaa tcttcacgcg gcaggcacca tcttcatcaa tattgccaac atagcgcaaa
     4141 acttttcctt catcacgggc cttcgccacg cgcgcggcaa agagatcgtc gagctgtgac
     4201 agattcgcca taaaagcggc aacatcaccc tcagcgttaa actctgcggg cagcacaggt
     4261 tcaatttcaa tatccgccag ctccagttca cgtcccgttt cacgagcgag aatcaatagc
     4321 ttacgcgcta catccatacc agaaagatca tctcgcggat ccggttcggt ataacccatt
     4381 tcccgcgcca gagtagtcgc ctcggagaaa ctcatgcctt cgtctaactt gccgaagata
     4441 taagaaagcg aacctgaaag aatgccggag aacttcatca attcatcacc agcattgagc
     4501 agattttgca ggttctcaat aaccggtaat ccagccccaa cgttggtgtc atagaggaat
     4561 ttacgccgcg atttttccgc cgcatgacgc aacagatggt agtaatccat cgacgaggtg
     4621 ttggcctttt tgttcggcgt gacaacgtgg aaaccttcgc gcaagaagtc ggcatattga
     4681 tccgccactg cctggctgga ggtgcagtca acaatgaccg ggttcagcag atgatattct
     4741 ttcacgaggc gaattaagcg cccgagatta aacggctctt tggcttgcgc cagttcttcc
     4801 tgccagtttt ccagatttag gccatgcaca ttggtgagca gtgccttcga gttggcaaca
     4861 ccgcagacac gtaagtcgat atgtttattc ttcagccagc tttgttgacg cttcagttgc
     4921 tccagcagcg caccgccaac gccaccgacg ccaatcacaa acacttcgat aacctgatcg
     4981 gtattgaaca gcatctgatg agtaacgcgc acgccagtgg tcgcatcatc gttatttacc
     5041 acgacagaga ttgagcgttc agaagatccc tgagcaatgg cgacaatgtt gatattggcg
     5101 cgggccagcg cggcaaagaa tttcgccgag atcccacgca aggtgcgcat accatcacct
     5161 accaccgaga taatggccag ccgttccgtc accgccagcg gctccagtaa gccttctttc
     5221 agttccaggt agaactcttc ctgcattgcc cgttcagctc gcacacagtc gctttgtgga
     5281 acgcagaaac tgatactgta ttcggaagat gattgcgtaa tcagcaccac ggaaatacgg
     5341 gcgcgtgaca ttgcagcaaa gacgcgcgcc gccatgccga ccattccttt catccccgga
     5401 ccagaaacgc tgaacattgc catattattc agattggaaa tgcctttgac cggtaattcg
     5461 tcttcatcac ggctggcacc aatgagcgta cctggtgctt gaggatttcc ggtattttta
     5521 atcaggcaag ggatctggaa ctgggcgatg ggggtaatgg tgcgggggtg aagaacttta
     5581 gcgccgaagt aggaaagctc catcgcttcc tggtaggaca tcgacttcaa caacctcgca
     5641 tcgggcacct gacgcgggtc gcaggtatag accccgtcaa cgtccgtcca aatctcgcaa
     5701 caatcggcgc gtaaacaggc agccagcacc gccgcggagt agtcggaacc gttacgtcca
     5761 agtaccacca gttcgccttt ttcattaccg gaggtgaaac ctgccatcaa caccatgtga
     5821 tcagccggaa tgcggcttgc cgcaatacgg cgggtggact cagcaatatc gacggtagat
     5881 tcgaggtaat gccccactgc cagcagtttt tcgaccggat cgataacggt aacgttgtga
     5941 ccacgagctt ctaacacgcc ggccataatg gcgatcgaca ttttctcgcc acggcaaatc
     6001 agcgcagcgt tgatgctatc cgggcactgc cccaacaaac taatgccatg caggacatgt
     6061 tttatttgga caaattcctg atcgacgaaa gttttcaatt gcgccagcgg gaaccccggc
     6121 tgggcggcgg cgagtcccgt caaaagttcg gcaaaaatac gttcggcatc gctgatattg
     6181 ggtaaagcat cctggccgct aatggttttt tcaatcatcg ccaccaggtg gttggtgatt
     6241 ttggcggggg cagagaggac ggtggccacc tgcccctgcc tggcattgct ttccagaata
     6301 tcggcaacac gcagaaaacg ttctgcattt gccactgatg taccgccgaa cttcaacact
     6361 cgcatggttg ttacctcgtt acctttggtc gaaaaaaagc ccgcactgtc aggtgcgggc
     6421 ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt
     6481 ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg tactctgtaa tttttatctg
     6541 tctgtgcgct atgcctatat tggttaaagt atttagtgac ctaagtcaat aaaattttaa
     6601 tttactcacg gcaggtaacc agttcagaaa ctgctatcag acactctttt tttaatccac
     6661 acagagacat attgcccgtt gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt
     6721 tttttatttg gtgatatttt tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc
     6781 tcgttgctct aaaagcccca ggcgttgttg taaccagtcg accagtttta tgtcatctgc
     6841 cactgccaga gtcgtcagca atgccatgac tcgttcgcgt aaagcttgca gttgatgttg
     6901 gtctgccgtt gtgtcacttt tcgtcggttg ttgtattaat gttgctaatt gatagcaata
     6961 gaccatcacc gcctgcccca gattgagcga aggataatcc gccaccatcg gcacaccagt
     7021 aagaacgtca gccaacgcta actcttcgtt agtcagcccg gaatcttcgc gaccaaacac
     7081 cagcgcggca tggctcatcc atgaagattt ttcctctaac agcagcacca gttctactgg
     7141 cgtggcgtag taatgatatt tcgcccgact gcgcgcagtg gtggcgacag tgaaatcgac
     7201 atcgtgtaac gattcaacca gtgtcgggaa aactttaata ttatcaataa tatcaccaga
     7261 tccatgtgcg acccagcgag tggctggctc cagatgtgcc tgactatcga caatccgcag
     7321 ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc ccaatatttt ctgctctggc
     7381 gggtgcgacc agaataatcg ttatacgcat attgccactc ttcttgacca aatgaccgcg
     7441 aacagggtga tcactgtcaa cttattacac ggtgcgaatt tacaaatcct taacgtaagt
     7501 cgcagaaaaa gccctttact tatcttaaaa aaggctaaac tatttcctga ctgtactaac
     7561 ggttgaattg ttaaaaaatg ctacatatcc ttctgtttac ttaggataat tttataaaaa
     7621 ataaatctcg acaattggat tcaccacgtt tattagttgt atgatgcaac tagttggatt
     7681 attaaaataa tgtgacgaaa gctagcattt agatacgatg atttcatcaa actgttaacg
     7741 tgctacaatt gaacttgata tatgtcaacg aagcgtagtt ttattgggtg tccggcccct
     7801 cttagcctgt tatgttgctg ttaaaatggt taggatgaca gccgtttttg acactgtcgg
     7861 gtcctgaggg aaagtaccca cgaccaagct aatgatgttg ttgacgttga tggaaagtgc
     7921 atcaagaacg caattacgta ctttagtcat gttacgccga tcatgttaat ttgcagcatg
     7981 catcaggcag gtcagggact tttgtacttc ctgtttcgat ttagttggca atttaggtag
     8041 caaacatgca gaccccgcac attcttatcg ttgaagacga gttggtaaca cgcaacacgt
     8101 tgaaaagtat tttcgaagcg gaaggctatg atgttttcga agcgacagat ggcgcggaaa
     8161 tgcatcagat cctctctgaa tatgacatca acctggtgat catggatatc aatctgccgg
     8221 gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt
     8281 tcctgactgg ccgtgacaac gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg
     8341 actacatcac caaaccgttc aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt
     8401 cccgtaccat gaatctgggt actgtcagcg aagaacgtcg tagcgttgaa agctacaagt
     8461 tcaatggttg ggaactggac atcaacagcc gttcgttgat cggccctgat ggcgagcagt
     8521 acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa
     8581 ttcagtctcg tgctgaactg ctgaagaaaa tgaccggccg tgagctgaaa ccacacgacc
     8641 gtactgtaga cgtgacgatc cgccgtattc gtaaacattt cgaatctacg ccggatacgc
     8701 cggaaatcat cgccaccatc cacggtgaag gttatcgctt ctgtggtgat ctggaagatt
     8761 aatcggcttt accaccgtca aaaaaaacgg cgctttttag cgccgttttt atttttcaac
     8821 cttatttcca gatacgtaat tgatcgtcca ttgtaacttc tttactggct ttcattttcg
     8881 gcagtgaaaa cgcataccag tcgatattac gagtcacaaa catcatgccg gccagcgcca
     8941 ccaccagcac actggttccc aacaacagcg cgctatcggc agagttgagc agtccccaca
     9001 tcacaccatc cagcaacaac agcgcgaggg taaacaacat gctgttgcgc cagcctttca
     9061 ataccgcctg caaataaata ccgttcatta acgccccaat cagactggcg attatccatg
     9121 ccacggtaaa accgatatgt tcagaaagcg ccagcaagag caaataaaac atcaccaatg
     9181 aaagccccac cagcaaatat tgcattgggt gtaaacgttg cgcggtgagc gtttcaaaaa
     9241 caaagaacgc cataaaagtc agtgcaatca gcagaatggc gtacttagtc gcccagtccg
     9301 ttaattggta ttgatcggct ggcgtcgtca ctgcgacgct aaacgcgggg aagttttccc
     9361 agccggtatc attgcctgaa gcaaaacgct caccgagatt attagcaaac cagctgcttt
     9421 gccattgcgc ctgaaaacct gactcgctaa cttcccgttt ggctggtaga aaatcaccta
     9481 aaaaactggg atgcggccag ttgctggtta agggcatttc gctattacgc ccggcaggca
     9541 ccacagaaag atcgccggta ccgcttaaat tcagtgccat attcagctgc aggttctgct
     9601 tccgccagtc cccttcgggt aaagggatat gcacgccctg cccgccttgc tctaacccgg
     9661 taccgggttc aatggtcagt gccgttccgt taacttcagg cgctttcacc acaccaatac
     9721 cacgcgcatc cccgacgcta atcacaataa agggcttgcc taagatgata tttggcgcgt
     9781 tgagctcgct aagacgcgaa acatcgaaat cggcttttag cgttaaatca ctgtgccaga
     9841 cctgaccggt ataaatccct atcttgcgtt cttccacgtt ctgattgcca tcaaccatca
     9901 atgactcagg taaccaaaaa tggataaaac ttcgtttccg ctccacggtt ttatcctctt
     9961 cctgcaccgt ataaagctcg gtcacaggaa tagcgataag cggcccaacg agtttttgcg
    10021 gcccgctggt actttggcga atcgcatctt ccacatcgct acggtaatcg gcacgttcga
    10081 caatcacttg ccgaatcagc attatcggaa tcaacaacag caatactgca ccaaacaagg
    10141 tggtcatttt ccagaacagg ggggatttca acatagtctt ctcctttgca atggcagcag
    10201 gatacgaagg ctatgtggga agaatttgaa gctatgtgaa gtgacggtga agtcgaagcg
    10261 aggccagcac gccaccttcc tgcacgttgc gcagcgtgac ttcgccgtta aacaaacggg
    10321 cgacctcact gacgaacgcc aaccccagac cgctgctttt ttgcccattt gcacgcggca
    10381 aagagtaaaa gcgttcaaaa atacgtgaca gcgcgtagtc aggaatacca ctaccggtat
    10441 ccagcacctt aagggcgacg tgttcctgat ccacttcggc gcttagcgtt atgcgaccgc
    10501 tctcgggggt aaaatcgatg gcgttatcca gtaaattccc cagcgcctgc tccagtaacg
    10561 ccggttcagc agcaacatta acctcagtgg gcataacatg tagagtgatg tttttttctg
    10621 ccaactgcac ggtgcgcgct tcgctgacgc ggcgaaataa tgccgccaca tcaacaacag
    10681 tcagaacgac ttcctgacga ttctccagtc ttgcctggcg tagtaacgtt tctaccaatg
    10741 cctgcatacg cgcattttgc gtcagaatgt tgtcggtaaa acgagccacc acttccggtg
    10801 gcggaccttc gcgtaaaatt tccgccgcgc cacgaatcgc cgccagtggg ctttttagct
    10861 catgggttaa cgcataaaca tactgctcaa tatagttttt cccttccagc ttcacgcgca
    10921 tactttccag cgcctgcgca agcttacgca actcgctact gccgagttca ggaagaggaa
    10981 cgggcttatt gtcagtgacg gaatcggcat agcgagtgag ccgggcaatg gagcggttga
    11041 tccaccaaac catgcctgcg ccaatcacca gtgcaatccc cagcaaaatg gcgctggccc
    11101 ataaaattcg ccgctcgcta cgcttaatga ccggagccat cgccgcgttc ggtttgccta
    11161 cgctcaaaac gccaataagc cgcgagccgt ccataatcgg cgcggcaaca tacatcaccg
    11221 aactttcggg atcggcagga ttttgcaacg tgctgcgcgc accatactga ccacgcaacg
    11281 ttagccagac gtcattccag cgcgaataat cctgcccaac ggctttattt gccgaatcga
    11341 acaatacttt accctgcgaa tcggtcatat agacatgata ttcattgcgc actttgttaa
    11401 tgccaccgat attggcgcga aacggacgat gttgtagctg attaaacgcc tgcgccagtt
    11461 gcccatgcgt tgggtcccca gagagcaaat cgggacgcgc cagctccgcc agcaacgttg
    11521 cggtgtcgat caacgtcccc tccgttgctc ttcgcacgcc cggtttaact tctttgacaa
    11581 aaatagccag tacgaaccag gccgccaccg ccaccagtaa aaaatagccc agcagcaacc
    11641 gcatgccgat acgcattaca ggcccctcag gctatatccc atgccgcgat gagtattaat
    11701 cggtgaaaga tcggggttga tggcacgcag cttggcatgc aacgttttaa tgtgggtatc
    11761 aacggtgcga tcgtaggtgt cctgcgcatc ttcccacacg ctatccatca gttgctggcg
    11821 ggaccagacg cggcctggcg acttgagtaa cgtcttcagc aataaaaact cataccgcgt
    11881 cagcgttaat ggcgtgtcaa accagctgat ctgcgccgcg ggttcattca attcaaaatg
    11941 accaatacgg atgacgggag acggcgacga gaacttcttc acccgacgca gtaaggtgcg
    12001 caccctggcg cacacttcgc ggggtgaaaa cggtttagcc acatagtcgt cagcgccaat
    12061 ttccagccca agcaggcgat cgacctcttc acttcgggcc gtcaggaaca gtacaggtaa
    12121 cgccggatgg agcgccagta actggctgca caattcaaag ccgctgatat caggcaaacc
    12181 aacgtcgaga atcatgacgt cgggaaccag ctggcgagct ttatccagca ccggcaagcc
    12241 tcgctcaaag acctcgacgg caaaaccttc ctgctgcaac atgtagacca gcgtgtcggc
    12301 tatcccttgc tcatcttcca ctaaccagac cgtttcccgt tgtataaatc gcctctgtta
    12361 ttgccgccac ggcatgacag gaaccgcgct aatcgcgttt ttcggcgaac cttctacaac
    12421 tttgtcggag taagccagat aagcgagcgc gttgcgtttg gcgtcataaa agcgcacgac
    12481 ctgtaacgac ttaaagacca gggacgtgcg ttttttgaat actacctcgc cctgagcttt
    12541 gccgttttta atacgatccg acagttcaat cggcccgact tgctgacaag aaatagccgc
    12601 atcggaggta tcttccgcca gacccaatcc ccctttaata ccacctgttt tcgcccggct
    12661 cacataacag gtgacatttt tcacatcggg atcgtcaaag gcttccacaa caattttgtg
    12721 atccgggccg atcattttaa atacggtgtc gaccgaacca atctcttctg catgagccaa
    12781 tggccccagc attatcaggc ttaaagacag gatcaaatgc ttgtatttca tattgttacc
    12841 atttttatct attacacgag caattagttc gtcacggtaa ataacatatt gattaagaac
    12901 aaaatctcaa ttcttttatt tccgtcaagc cctacaacat actctactaa gaaaaaaaca
    12961 ctgaatgcta aaacagcaaa aaatgctatt atccaattac ctgatgtcag gtgctcgttg
    13021 ttgaaaggat gaggatattt tatggatcag gccggcatta ttcgcgacct tttaatctgg
    13081 ctggaaggtc atctggatca gcccctgtcg ctcgacaatg tagcggcgaa agcaggttat
    13141 tccaagtggc acttacagag aatgtttaaa gatgtcactg gccatgctat tggcgcgtat
    13201 attcgtgctc gtcgtttgtc gaaatcggcg gtcgcactac gcctgactgc gcgtccgatt
    13261 ctggacatcg cgctgcaata ccgcttcgac tctcaacaga catttacccg cgcattcaag
    13321 aagcagtttg cccagactcc tgcactttac cgccgttctc ctgaatggag cgcctttggt
    13381 attcgcccgc cgctacgtct gggtgaattc acaatgccag agcacaaatt tgtcaccctg
    13441 gaagatacgc cgctgattgg tgttacccag agctactcct gttcgctgga gcaaatctct
    13501 gatttccgcc atgaaatgcg ttatcagttc tggcacgatt ttctcggcaa cgcgccgacc
    13561 attccgccag tgctctacgg cctgaatgaa acgcgtccga gtcaggataa agacgacgaa
    13621 caagaggtat tctataccac cgcgttagcc caggatcagg cagatggcta tgtactgacg
    13681 gggcatccgg tgatgctgca gggcggcgaa tatgtgatgt ttacctatga aggtctggga
    13741 accggcgtgc aggagtttat cctgacggta tacggaacgt gcatgccaat gctcaacctg
    13801 acgcgccgta aaggtcagga tattgagcga tactacccgg cagaagacgc caaagcggga
    13861 gatcgcccaa ttaatctgcg ctgtgaactg ctgattccta tccgccgtta acgctgcagc
    13921 tcatctaatg caggggcgtc cagatgcgag atgtcccctg cggtttcaac gacccagcca
    13981 gacgccagcc acaggctttc ctgataatcc acgcgcgaaa tagaacagtt acgcagacgt
    14041 aagcggcgcg ctgcccatgc tggtaatccg agaatcgtac tcaccagaca tcccagtgca
    14101 ataccatggc tcaccagcag cggtcggctt ccctgcggta agtcccggca agattccagt
    14161 gccgcattga cgcgatcgct gagctcctgc attgactcgc cttcaggaat acgcccgtca
    14221 acggtgccat tgaccagctg ccgacgccag ttctcttctt cttcggtcag agaatcgata
    14281 tgtctttttt ccagcacacc catgtttaat tcacgcaggc gagaatcaaa gatgatgtca
    14341 cagccgcagg cctgggcgat gatttccgcc gtacgccggg tgcgtcctaa atcgctactg
    14401 ataatatgcg taatgccaag ctctttggca cgggttgcca cctgcatcgc ctgttgctca
    14461 cctttggcgg tcagcgggct gtcagactgg ccctgaatac gtcgctcggc gttccactgc
    14521 gtttcaccgt ggcggactag gtatacctgt aacatgcttt ttttccgtat actgctgtaa
    14581 cgttaatttt ctgagtaatg ctgattatgc accaagttgt ctgtgcgacc accaatcccg
    14641 ctaaaattca ggccattctg caggcatttc acgagatctt cggcgaagga tcctgccata
    14701 ttgcatccgt tgccgtcgag agcggtgtac cggaacagcc ctttggcagt gaggaaacgc
    14761 gcgctggcgc acgaaatcgg gtagcaaatg cccgccgttt acttccagag gctgattttt
    14821 gggtggcgat tgaagctggc atcgatggtg acagcacttt cagttgggtg gtgattgaaa
    14881 acaccagcca gcgcggcgag gcgcgttcgg ctactttgcc gttacctgcg gtgattctgc
    14941 agaaagtgcg tgaaggtgag gcgcttggtc ccgtgatgtc gcgttatacc ggtattgatg
    15001 agattggtcg taaggaaggg gcgatcggcg tgtttaccgc cgggaaactc actcgcgcca
    15061 gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt tcacaatgcc gtgtatcagg
    15121 cattgtagga cggataaggc gttcacgccg catccggcat tcggtgcacg atgcctgatg
    15181 cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc ttttcagcaa cacctcttcc
    15241 agccactggc gcagctccac gggcgcggct ttcaggctgt tagatccacg cgtaatcgtc
    15301 gcgatgcccg cgccgagttc attttttaac tcacgctgac tcatttcgcc acgcaacagc
    15361 tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct catctggcgt cagcatcagg
    15421 tttaacaacg gtaaatggag atcgttttgg taggcattct taagcaggtc gacaaaacgt
    15481 aaccactcct ggtgacgctg ttctgccatc gctgctgaat agggtgattg ttgggccata
    15541 atatgtcgcc attgttagcg ggggaagcaa aatgcctccc ccggttgtac tcgctaaaga
    15601 gtacgatagc atatcataaa cgtgcggatc agtaacgacg tccccattcc gtggcgctca
    15661 tcaacgtcgg tttatccccc atgaaataac ggtagtaagc gtcataagcc agcacgttct
    15721 tcacataacc gcgcgtctcg gagaacggaa tactctcgac aaatgccaca gcgtcgatac
    15781 gcccggcgct gttgccaagc caggttcgca cccgtccagg tccggcgtta taagctgctg
    15841 aggagaaaat acggttattg ccaaactgct gataaacata ttgcaggtaa ctggtgccaa
    15901 tgttgatatt cgtttccgga tccagcaatt gcccagggct gctataacca ggaatagaga
    15961 acatcttcac cgtatgggtc gctgtaccag gcataatctg catcaggccg ctggccccta
    16021 ccggtgattt cactttcgga ttccaggcgc tctcctgacg ggcaatcgcc atcgcatagc
    16081 tttgcgggat ctctttccca ctggtgtaac gtttgaaaag atcgttgtaa gccagcggga
    16141 atcgctcttc cagatgatcc caaagcttcc cggcgatcgt tgcctgaacg ctaagatccc
    16201 accattggtt attgaaagca taccgagcca gttgagcctg ctctgttttt gacttgctct
    16261 tcaccagatt ggcccactcg ctacgcgcgg tgttatcgag attccagtac atcaactcgc
    16321 gcacgcgcgc catctccggc ccctgagtca gggcgctgtc aacattctgc ggcgctttat
    16381 caatcttcag ctcatactct tctccgatgc gttgtgctgc aaccatcggg tagaaaccac
    16441 gctgttgcat gagttgatgc aaaatctctt tcgcttcagc ttcacgtccg cgttccagca
    16501 ataaatccgc ctgccagtaa cgccattcat ctttctcttt tgcttccatc ggcagacgcg
    16561 ccagccaggt attcaggccg cgacgatcgc cggtgccaag tgccattcgc acacggcgtt
    16621 caatcaacga cgtggattgc gagcgcataa tggcgtcatc gcgccatttc gcctgctcgt
    16681 cggtgacatc gttgcccatc aaacgccagg cgacgatatc gcgcagctcc tgaatctgat
    16741 cttcattaag ctgctgcgcc tgggcaagcg acgggatcat cagccgtgca ttttcagcat
    16801 cctgccgcgc cacactggca aacgccaccg ctgccatttg acgggtaaaa tcggtcgctc
    16861 cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt cgccagtgaa atgattgccg
    16921 aggcgatagt ctggtaatcg gcaggcatct gccctgccag cactgttacc aggccagtgt
    16981 tacccgcttt catcgccaga cggatacgct ctaaatacgc cagcggatct tgtttacctg
    17041 acgcacgcca gacgctaaat aacttgtcac aggcgttagg ctggctcttg ccggttagcc
    17101 acagctcttt cgccccttgc caggcttctt cactctgccc ggtgttccat ttcgcatagt
    17161 agtaattaca ttgcgcttcg gtagttccgg gcttttccgg gctaaaggct aacaagccac
    17221 gccagtcttc acgccgcgcc agttcattga cgaaacgaga ttgcagcgtg cgagcgggag
    17281 gaagcgtggg gttagcgcga acaaagttag tgaccgtcac cgccggttga ttcatcagat
    17341 catcggtgat ctggcggtat tccaggtagg gataaagcgg ataatccttc agtccaggca
    17401 tcatttgttc gaccacatcc atttgtcgat tatcccaggc ctgcttgatt tgcgcgtaac
    17461 ggctacgctg ctcatccagt gagtcggctc gcgccacgct gctgaccgtc agcagacaga
    17521 caccggcagc caacagccgc caggtaactt gtttggcttt ttccacaagc gcatcctcta
    17581 agtgtaaata catcaatgca gcgtcatgcc gcgtaataga tttatgctaa ccagtcattg
    17641 ccgtttacgc cacgttacgg acactttttt acttttactg cgaaggtgat cgaccaactg
    17701 ggattaggcg gggaaaaagg ctacacttcg cctttgaaaa ctcatcatca cgataaacat
    17761 agaggcgaag tccaacgtgg ctcaattcgt ttataccatg catcgtgtcg gcaaagttgt
    17821 tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt ttcttccctg gggcaaaaat
    17881 tggtgtcctg ggtctgaacg gcgcgggtaa gtctaccctg ctgcgcatta tggcaggcat
    17941 tgataaagac atcgaaggtg aagcgcgtcc gcagccagac atcaagattg gttacctgcc
    18001 gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag tccattgaag aagcggtttc
    18061 tgaagtggtt aacgccctga aacgcctgga tgaagtgtat gcgctgtacg ccgatccgga
    18121 tgccgatttt gacaagctgg ccgctgaaca aggccgtctg gaagagatca ttcaggctca
    18181 cgacggtcat aacctgaacg tacagctgga gcgtgcggcg gatgcgctac gtctgccgga
    18241 ctgggacgcg aaaatcgcta acctctccgg tggtgagcgt cgtcgcgtag cgttgtgccg
    18301 cctgctgctg gaaaaaccag acatgctgct gctcgacgaa ccgaccaacc acctggatgc
    18361 cgaatccgtg gcctggctgg aacgcttcct gcacgacttc gagggcaccg tggtggcgat
    18421 tacccacgac cgttacttcc tcgataacgt tgcaggctgg atcctcgaac ttgaccgcgg
    18481 tgaaggtatt ccgtgggaag gcaactactc ctcctggctg gagcagaaag atcagcgcct
    18541 ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg atcgagaaag agctggagtg
    18601 ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa gcacgtctgg cacgctttga
    18661 agagctgaac agcaccgaat atcagaaacg taacgaaacc aacgaactgt ttattccacc
    18721 tggaccgcgt ctgggcgata aagtgctgga agtcagcaac ctgcgtaaat cctacggtga
    18781 tcgcctgctg attgatgacc tgagcttctc gatcccgaaa ggggcaatcg tcgggatcat
    18841 cggtccgaac ggcgcgggta aatcgaccct gttccgtatg atctctggtc aggaacagcc
    18901 ggacagcggc accatcactt taggtgaaac ggtgaaactg gcatcggttg atcagttccg
    18961 tgactcaatg gataacagca aaaccgtttg ggaagaagtt tccggcgggc tggatattat
    19021 gaagatcggc aacaccgaga tgccaagccg cgcctacgtt ggccgcttta actttaaagg
    19081 ggttgatcag ggtaaacgcg ttggtgaact ttccggtggt gagcgcggtc gtctgcatct
    19141 ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc gacgaaccaa ccaacgacct
    19201 ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg gagttcccgg gctgtgcgat
    19261 ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc acgcacatcc tggactacca
    19321 ggatgaaggt aaagttgagt tcttcgaagg taactttact gagtacgaag agtacaagaa
    19381 acgcacgctg ggcgcagacg cactggagcc gaagcgtatc aagtacaagc gtattgcgaa
    19441 gtaatgcgta aaatgccgga tgcggcgtga acgccttatc cggcctacaa accaagaaat
    19501 ttcaatacgt tgtgaatctc caggcctgat aagacgcggt aagcgtcgca tcaggcattg
    19561 agcaccgaat gccggatacg gcgtgaacgc cttttccggc ctacaatagc cttagtatcc
    19621 gcaatttcgt agcgggtcac ccgtgctgat tcttttaaac cttgtttaat ctcgacagcc
    19681 tcttccagag aggttttcaa ctcatcaaaa aacttatttt atacctcgcc tttgagccgt
    19741 ttcatcgcgg ttatctctgc tcccccatca tctcccgcac cagctccacg cagcgcagga
    19801 aacggctgtc ataatcgtcc tcttcaaccc gcacgaattc gatattgttc tcttccagca
    19861 tctccaccag caagttctgg aactctttgc gatccaccga actgccgagg ctgcgtaaac
    19921 catccgccac ccacggcgtg ttgttctcca gcaggatcac cagatcgaaa cggtattcat
    19981 caatcaacgc ctgtacgaac ggatgctcac gcccttcgta ctttttgcag aacgcctggg
    20041 tagtgacaaa atcagtgtca ataaacgcca ctttattggc atatttcact gcaaaatcaa
    20101 tgtattgtgc gtggcccagc gcgattttat cgtaatcgga atactgcaat gcgatctcat
    20161 caccgccgag gtgtgaaaag acataatcgc gaccatattc ccacgcactg gtggtattga
    20221 agatattggc aagtttgttt accagggtgg atttaccgct cgactcgcca ccaaggatcg
    20281 ccacggtacg tacaaagaac ggcttcactt ccgtaggaat atattcccag tagcggaacg
    20341 ggttttcgcg gatctgcgca ccgctgatac tcataaaggt acgtttcgga tcgaccagca
    20401 ccgtatcgat ccccagatgt tccatatact gtggcgcatc ggcttcttcc gaggtgtaga
    20461 tcaggtccgg ctgaatcccc ttttcagcca taaacttttt gatgccgttg ctccacacat
    20521 cccagccgtg cggatacggc tccatgccct cctcgttgaa agcatgaatg cgaatatttt
    20581 tctgatattt aaaagtttgt aataaccagc gcagacgatc cggcaccgtg ggctgctgcg
    20641 acatggcgct gtcttcaaac aacgcgcggt cgcgggtatc gtcaaaaccc ataatgatat
    20701 gcagctcgtc aacctggcta caggcgcgct ggataaggta gatatgtccg gtatgcagcg
    20761 ggtagaactt accgaatacg acaccaatgg ttttcttctg tcggggaaat tcaagcccca
    20821 aaaaacggtg caacgcctcc agcttttgcg cgctggggct tttaattttg gcattcagta
    20881 actggcttaa ataccctttg gtcataccgc tggcatcagc tacctgctgt agcgtgcagc
    20941 cctgttgctt gatggcagtt ttcaggtaat caaatgacga catatctccc tccgtatctc
    21001 tcattataag tcgtcgaaca cgctaagcgc gtcggagagt tttttaacgc caaaaatctg
    21061 catcccttcc ggcgcttttt tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc
    21121 gtgtttcgcc gcttctgaaa ttcgttcctg accgctgggc accgggcgga tctcccctgc
    21181 cagcccgact tcaccaaaca ccaccagatc ctgtggcagc ggtctgtcac gcaggctgga
    21241 aaccatcgcc agcagtaacg ctaaatcggc actggtttcg gttaccttca cgccgccgac
    21301 cacgttcaca aatacatcct gatcggccat ttgcagacca ccgtgacggt gcaagacagc
    21361 cagcaggatt gccagacggt tttgttccag ccccactgcc acgcggcgcg gattcgccat
    21421 catcgagtga tcgaccagcg cctgaatctc caccagcaac ggacgcgttc cttcccacac
    21481 caccatcacg gagctgccgg aggtcacttc atctccgcga cttaagaaaa ttgccgaagg
    21541 gttgctgact tcacgcagcc cctgttcggt catcgcgaag acgcccagct cattcaccgc
    21601 gccgaagcgg tttttatggc tgcgcaaggt gcgaaaacgg gagtcggcat caccatccag
    21661 aagcaccgaa cagtcgatac agtgttccag cactttaggg ccagccagcg agccatcttt
    21721 ggttacgtgc ccaaccatga caatcgccac accgcgcgtt ttggcgaagc gcgtcagata
    21781 agccgccgtt tcacgcacct gcgccacgct accaggcgat gattgtacat ccgccatatg
    21841 catcacctgg atcgagtcaa ttaccatcag tttcggttgc tcttcttcgg caatcaggca
    21901 gatctgttcg atgctggttt ccgacaacat attgagattg tcagtcggca ggccaaggcg
    21961 atgagcgcgc attgccacct gttgcagcga ctcttcgcca gtgacataca gcgttttcat
    22021 ctgctgggcc agtttgcaca gcgtttgcag tagcagcgtg gatttccccg cacctgggtt
    22081 accgccaatc agaatggcgc ttcccggcac cacgccgccg cctagtacgc ggtcgaactc
    22141 tttaaatccg gtggaaaaac gcggcagctc ttcaaggctg atatcggaga gtttctggac
    22201 ttttgccacc ccggcgctac cggcatagcc gctgagacgc tcgttacgcg ccaccgttgg
    22261 cgacgcagca agacgcacct cggtgatggt gttccaggca tgacaggcac tgcactgccc
    22321 ctgccagcgc ggataatcgg ccccgcattc attacaaaca aaggcgcgtt ttggagcttt
    22381 tgccacggtg tacctcgtta atgctgtgcc gcccgcagga tggcgggcga gcaattactt
    22441 ctgattcagg ctgccagaga ggatgcagaa tacccccatc aggtcagcgt gacggatggt
    22501 gacttccgcc ttttcattca cttttggctt ggcatggtag gcaatcccca gccctgctgc
    22561 tttgatcatc ggcaggtcat tagctccatc gccaatcgcc acggtctgcg ccagcgggat
    22621 ttcatactcc tgcgcgaggc gagtcaaagt tttcgctttg tactgcgcgt ctacgatgtc
    22681 gccgatcaca ttgccggtaa atttaccgtc catgatctcc agttcattgg ctaccacggc
    22741 ggtcaggcgc agcttgtcgc gcagatattc agcaaagaaa gtaaagccgc cggaggcaat
    22801 cgccactttc cagcccagcg tttccagctt gagcaccagt tgcgttaagc ctggcatcag
    22861 cggcagattt tcacgcacct gttgcagaat attggcgtca gcgcctttca gcgtcgccac
    22921 acggctgcgc aggctggcgg taaaatcgag ttcgccgcgc atcgcccgtt cggttacttc
    22981 cgccaccatc tcgcccgttc cggccagttt ggcaatttca tcaatacatt caatctggat
    23041 ggcggtggaa tccatgtcca tcaccagcaa acccggcgtg cgcaggtgcg ggattttccc
    23101 cagcggggcg acatccagtt gcgcttcatg ggccaggcgc gtagcccgtg cggtgagtga
    23161 acctgccaga cgaatcacct gataatcttc cacgcaccag gcggcaacaa tcaccatcgc
    23221 cgcacccagt ttgctctggt attgggtcag acgctgttta tccagcccac gaccatacag
    23281 cagccagccg ctacgacctg cgtggtaatc cagtggcatc acttcatcac cacttaatga
    23341 aagaggcaga ccaggccata aagagacatc ttcaggcagg tcgcaccagg taatgttagg
    23401 cattaaggat cctgtaaaat cgttcgaagc agggaaaata acgcatgagg ctaccttgta
    23461 tccattgctt ctggcaacat taagtctcaa attttcaaag ggtggaagat ggctcgcaca
    23521 aaactgaaat tccggctgca tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg
    23581 gcgctgatgc agggagcgtc atggtttagt caaaaccacc agcgacaacg taatccacag
    23641 ctggaagaac tggcccgcac cctggcgcgt caggtaacgc tgaacgttgc accgctgatg
    23701 cgtaccgact caccggatga aaaacgcatt caggcgatcc tcgatcagtt aacggatgaa
    23761 agccgtatcc tcgacgcggg tgtgtatgac gaacaaggcg atcttatcgc acgttctggc
    23821 gaaagcgtcg aagtgcgcga ccggctggcg ctcgacggta aaaaagcggg cggctatttt
    23881 aaccagcaga ttgtcgaacc aattgccggg aaaaacggac cgctcggcta tctgcgcctg
    23941 acgctcgaca cccatacgct cgccaccgaa gcccaacagg tggataacac cactaacatt
    24001 ttacgcctga tgttgctgct ttcactggca atcggcgtag tgctgacccg cacgctgcta
    24061 cagggtaaac gtacccgctg gcagcaatcg cccttcctgt taaccgccag caaaccggtg
    24121 ccggaagagg aagaaagcga gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt
    24181 atgtccacat tacgcctgct catctctgac tcttacgacc cgtggtttaa cctggcggtg
    24241 gaggagtgta tttttcgcca gatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat
    24301 gccgacacgg tagtgattgg tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg
    24361 atggaagaag ataacgtccg cctggcacgg cgcagtagcg gtggcggcgc ggtgttccac
    24421 gatctcggca atacctgctt tacctttatg gctggcaagc cggagtacga taaaaccatc
    24481 tccacgtcga ttgtgctcaa tgcgctgaac gcgcttggcg tcagcgccga agcgtccggg
    24541 cgtaacgatc tggtggtgaa aaccgccgaa ggcgaccgca aagtctcagg atcggcctat
    24601 cgcgaaacca aagatcgtgg cttccaccac ggcaccttgc tgctcaatgc cgaccttagc
    24661 cgcctggcaa actatctcaa tccggataaa aagaaactgg cggcgaaagg cattacctca
    24721 gtgcgttccc gcgtgaccaa cctcaccgag ctgctgccgg ggatcaccca tgagcaggtt
    24781 tgcgaggcca taaccaaggc ctttttcgcc cattatggcg agcgtgtaga agcggaaatc
    24841 atctccccgg acaaaacgcc agacttgcca aacttcgccg aaatctttgc ccgtcagagt
    24901 agctgggaat ggaacttcgg tcaggctccg gcattctcgc atctgctgga tgaacgcttt
    24961 agctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac ccgcgcccag
    25021 gtgtttaccg acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg actgcaaggc
    25081 tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg
    25141 gaccaggaaa aagagctacg ggagttgtcg acgtggatag cgggggcggt aaggtaactc
    25201 gctgattaaa ataccaaagg aagggaatat gatcgatctg gaaaaccagg agcgggagat
    25261 tatcaattta atgttaagcc agcgtatttc ctggctagcc gccgtgcgta tcaggcataa
    25321 attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg attaattctc taaaatagat
    25381 cgaaaagacg gaacggttta gctcaaacat caaaagcaaa atggcagaaa tttatggttg
    25441 tccaccagag ttgctgatat gcccttcctg gatgacggct gaacataaat gaatagatt
//
LOCUS       DAJTSL010000045        25242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTSL010000045 DAJTSL010000000
VERSION     DAJTSL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.045"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..403)
                     /locus_tag="OO433_001666"
     CDS             complement(<1..403)
                     /locus_tag="OO433_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6050179.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGTIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLP"
     gene            782..1093
                     /gene="rpsJ"
                     /locus_tag="OO433_001667"
     CDS             782..1093
                     /gene="rpsJ"
                     /locus_tag="OO433_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6050180.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1126..1755
                     /gene="rplC"
                     /locus_tag="OO433_001668"
     CDS             1126..1755
                     /gene="rplC"
                     /locus_tag="OO433_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6050181.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1766..2371
                     /gene="rplD"
                     /locus_tag="OO433_001669"
     CDS             1766..2371
                     /gene="rplD"
                     /locus_tag="OO433_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6050182.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2368..2670
                     /gene="rplW"
                     /locus_tag="OO433_001670"
     CDS             2368..2670
                     /gene="rplW"
                     /locus_tag="OO433_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6050183.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2688..3509
                     /gene="rplB"
                     /locus_tag="OO433_001671"
     CDS             2688..3509
                     /gene="rplB"
                     /locus_tag="OO433_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6050184.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3526..3804
                     /gene="rpsS"
                     /locus_tag="OO433_001672"
     CDS             3526..3804
                     /gene="rpsS"
                     /locus_tag="OO433_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6050185.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3819..4151
                     /gene="rplV"
                     /locus_tag="OO433_001673"
     CDS             3819..4151
                     /gene="rplV"
                     /locus_tag="OO433_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6050186.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4169..4870
                     /gene="rpsC"
                     /locus_tag="OO433_001674"
     CDS             4169..4870
                     /gene="rpsC"
                     /locus_tag="OO433_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6050187.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4883..5293
                     /gene="rplP"
                     /locus_tag="OO433_001675"
     CDS             4883..5293
                     /gene="rplP"
                     /locus_tag="OO433_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6050188.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5293..5484
                     /gene="rpmC"
                     /locus_tag="OO433_001676"
     CDS             5293..5484
                     /gene="rpmC"
                     /locus_tag="OO433_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6050189.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5484..5738
                     /gene="rpsQ"
                     /locus_tag="OO433_001677"
     CDS             5484..5738
                     /gene="rpsQ"
                     /locus_tag="OO433_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6050190.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5903..6274
                     /gene="rplN"
                     /locus_tag="OO433_001678"
     CDS             5903..6274
                     /gene="rplN"
                     /locus_tag="OO433_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6050191.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6285..6599
                     /gene="rplX"
                     /locus_tag="OO433_001679"
     CDS             6285..6599
                     /gene="rplX"
                     /locus_tag="OO433_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6050192.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6614..7153
                     /gene="rplE"
                     /locus_tag="OO433_001680"
     CDS             6614..7153
                     /gene="rplE"
                     /locus_tag="OO433_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6050193.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7168..7473
                     /gene="rpsN"
                     /locus_tag="OO433_001681"
     CDS             7168..7473
                     /gene="rpsN"
                     /locus_tag="OO433_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6050194.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7507..7899
                     /gene="rpsH"
                     /locus_tag="OO433_001682"
     CDS             7507..7899
                     /gene="rpsH"
                     /locus_tag="OO433_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6050195.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7912..8445
                     /gene="rplF"
                     /locus_tag="OO433_001683"
     CDS             7912..8445
                     /gene="rplF"
                     /locus_tag="OO433_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6050196.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRIKEAKKK"
     gene            8455..8808
                     /gene="rplR"
                     /locus_tag="OO433_001684"
     CDS             8455..8808
                     /gene="rplR"
                     /locus_tag="OO433_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6050197.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8823..9326
                     /gene="rpsE"
                     /locus_tag="OO433_001685"
     CDS             8823..9326
                     /gene="rpsE"
                     /locus_tag="OO433_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6050198.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9330..9509
                     /gene="rpmD"
                     /locus_tag="OO433_001686"
     CDS             9330..9509
                     /gene="rpmD"
                     /locus_tag="OO433_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6050199.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9513..9947
                     /gene="rplO"
                     /locus_tag="OO433_001687"
     CDS             9513..9947
                     /gene="rplO"
                     /locus_tag="OO433_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6050200.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9955..11286
                     /gene="secY"
                     /locus_tag="OO433_001688"
     CDS             9955..11286
                     /gene="secY"
                     /locus_tag="OO433_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6050201.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11318..11434
                     /gene="rpmJ"
                     /locus_tag="OO433_001689"
     CDS             11318..11434
                     /gene="rpmJ"
                     /locus_tag="OO433_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6050202.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11581..11937
                     /gene="rpsM"
                     /locus_tag="OO433_001690"
     CDS             11581..11937
                     /gene="rpsM"
                     /locus_tag="OO433_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6050203.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11954..12343
                     /gene="rpsK"
                     /locus_tag="OO433_001691"
     CDS             11954..12343
                     /gene="rpsK"
                     /locus_tag="OO433_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6050204.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12377..12997
                     /gene="rpsD"
                     /locus_tag="OO433_001692"
     CDS             12377..12997
                     /gene="rpsD"
                     /locus_tag="OO433_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6050205.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13023..14012
                     /gene="rpoA"
                     /locus_tag="OO433_001693"
     CDS             13023..14012
                     /gene="rpoA"
                     /locus_tag="OO433_001693"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6050206.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14053..14436
                     /gene="rplQ"
                     /locus_tag="OO433_001694"
     CDS             14053..14436
                     /gene="rplQ"
                     /locus_tag="OO433_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6050207.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14543..14911
                     /locus_tag="OO433_001695"
     CDS             14543..14911
                     /locus_tag="OO433_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR6050208.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGF
                     STDFLRGDYADKLLDKINDN"
     gene            14922..15347
                     /gene="zntR"
                     /locus_tag="OO433_001696"
     CDS             14922..15347
                     /gene="zntR"
                     /locus_tag="OO433_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR6050209.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15403..15621
                     /gene="arfA"
                     /locus_tag="OO433_001697"
     CDS             15403..15621
                     /gene="arfA"
                     /locus_tag="OO433_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR6050210.1"
                     /translation="MSRYQHTKGQIRDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="OO433_001698"
     CDS             complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="OO433_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR6050211.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="OO433_001699"
     CDS             complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="OO433_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6050212.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="OO433_001700"
     CDS             complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="OO433_001700"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6050213.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAKFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRHH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="OO433_001701"
     CDS             complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="OO433_001701"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6050214.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERYIRAFNPWPMSWLEIERQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19853..20362)
                     /gene="def"
                     /locus_tag="OO433_001702"
     CDS             complement(19853..20362)
                     /gene="def"
                     /locus_tag="OO433_001702"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6050215.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20492..21616
                     /gene="dprA"
                     /locus_tag="OO433_001703"
     CDS             20492..21616
                     /gene="dprA"
                     /locus_tag="OO433_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6050216.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     YALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVSPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21588..22061
                     /locus_tag="OO433_001704"
     CDS             21588..22061
                     /locus_tag="OO433_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6050217.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLTEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLEI
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22090..22632
                     /locus_tag="OO433_001705"
     CDS             22090..22632
                     /locus_tag="OO433_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR6050218.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22637..23209
                     /gene="tsaC"
                     /locus_tag="OO433_001706"
     CDS             22637..23209
                     /gene="tsaC"
                     /locus_tag="OO433_001706"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6050219.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVLGETGGRLNPSEIRDALTGELFRQG"
     gene            23214..24032
                     /gene="aroE"
                     /locus_tag="OO433_001707"
     CDS             23214..24032
                     /gene="aroE"
                     /locus_tag="OO433_001707"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6050220.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24029..24286
                     /locus_tag="OO433_001708"
     CDS             24029..24286
                     /locus_tag="OO433_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6050221.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24262..24816)
                     /locus_tag="OO433_001709"
     CDS             complement(24262..24816)
                     /locus_tag="OO433_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6050222.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGGVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6457 a         5996 c         6569 g         6220 t
ORIGIN      
        1 aaggcagcgg gttttttaat gattcttttc ctctcatgag cagaccaata actacggctc
       61 cgcaagcgaa tgaggcagca aggaaaacca gtcgtggtaa gaaggcccat gaatgccagg
      121 cgccaagggc ggcgagaaac ttcacatcac catagcctaa tccttcttta tgtcgcagta
      181 tgcgataccc ccagtaaatg acagcgaatg tgccgtaacc aacaattgtc ccccataacg
      241 catcggctaa accatcagga tgacaaactt gatagaacaa aagacctgac cagagtaacg
      301 ggcacgtaaa tctgtcaggt aacaagccat gctttgcatc ccagaaaaaa agtagggctg
      361 ataaacaagc gtacaaaatc agaaagggaa gggtagtgtc cataaaacat cctctgtaat
      421 tggagtatca ccatcgaata actccggatg ctcagcaaca gagagagcgc tgatttgtta
      481 acctgattcc ggtgaaaaac gctatcttca caaccttaca aatttcgtgg aatgcagctc
      541 gcaatctgcg cgttgcgatt aatttctacc cgatgcttgc gtcaggctca gatttgcgta
      601 taatgcgcgg gcttgtcgta gttgacagca ggttcaatct gaacctcagt tagccgaatt
      661 tggctaccta acaatgctcc caatcgggga gctacgtaag aacggttaca ctctcccatc
      721 aatcgtaatg ggtctgagga gtaatcattt tcgtttataa aataattgga gctctggtct
      781 catgcagaac caaagaatcc gtatccgcct gaaagcgttt gatcatcgtc tgatcgatca
      841 agcaaccgcg gaaatcgtcg agactgccaa gcgcactggt gcgcaggtcc gtggtccgat
      901 cccgctgccg acacgcaaag agcgcttcac tgttctgatc tccccgcacg tcaacaaaga
      961 cgcgcgcgat cagtacgaaa tccgtactca cttgcgtctg gttgacatcg ttgagccaac
     1021 cgagaaaacc gttgatgctc tgatgcgtct ggatctggct gccggtgtag acgtgcagat
     1081 cagcctgggt taatcaggtc attgagcgat tgagaggttg aaacaatgat tggtttagtc
     1141 ggtaaaaaag tgggtatgac ccgtatcttc acagaagacg gcgtttctat cccagtaacc
     1201 gtaatcgaag ttgaagcaaa ccgcgttact caggttaaag acctggctaa cgatggctac
     1261 cgtgctattc aggtgaccac cggtgctaaa aaagctaacc gtgtgaccaa gcctgaagct
     1321 ggccacttcg ctaaagctgg cgtagaagct ggccgtggtc tgtgggaatt ccgcctggct
     1381 gaaggcgaag agttcactgt aggtcagagc attagcgttg aactgtttgc tgacgttaaa
     1441 aaagttgacg taactggcac ctctaaaggt aaaggtttcg caggtaccgt taagcgctgg
     1501 aacttccgta cccaggacgc tactcacggt aactccttgt ctcaccgcgt tccgggttct
     1561 atcggtcaga accagactcc gggcaaagtg ttcaaaggca agaaaatggc aggtcagatg
     1621 ggtaacgaac gtgtaaccgt tcagagcctt gacgtagtac gcgttgacgc tgagcgcaac
     1681 ctgctgctgg ttaaaggtgc tgtcccgggt gcaaccggta gcgacctgat cgttaaacca
     1741 gctgtgaagg cgtaaggaga tagcaatgga attagtattg aaagacgcgc agagcgcgct
     1801 gactgtttcc gaaactacct tcggtcgtga tttcaacgaa gcgctggttc accaggttgt
     1861 tgttgcttat gcagctggtg ctcgtcaggg tactcgtgct cagaagactc gtgctgaagt
     1921 aactggttcc ggtaaaaaac cgtggcgcca gaaaggcacc ggccgtgcgc gttctggttc
     1981 tatcaagagc ccgatctggc gttctggtgg cgtgaccttt gctgctcgtc cgcaggacca
     2041 cagtcaaaaa gttaacaaga agatgtaccg cggcgcgctg aaaagcatcc tgtccgaact
     2101 ggtacgtcag gatcgtctga tcgttgtcga gaagttctct gtagaagcgc cgaaaactaa
     2161 gctgctggca cagaaactga aagacatggc tctggaagat gtgctgatca tcaccggtga
     2221 gctggacgaa aacctgttcc tggctgcgcg caacctgcac aaggttgacg tacgcgatgc
     2281 aactggtatc gacccggtta gcctgatcgc cttcgacaaa gtcgtaatga ctgctgatgc
     2341 tgttaagcaa gttgaggaga tgctggcatg attcgtgaag aacgtctgct gaaggtgctg
     2401 cgtgcaccgc acgtttctga aaaagcgtct actgcgatgg aaaaatccaa caccatcgta
     2461 ctcaaagttg ctaaagacgc gaccaaagca gaaatcaaag ctgctgtgca gaaactgttt
     2521 gaagtcgaag tcgaagtcgt taacaccctg gtagttaaag ggaaagttaa acgtcacgga
     2581 cagcgtatcg gtcgtcgtag cgactggaaa aaagcttacg tcaccctgaa agaaggccag
     2641 aatctggact tcgttggcgg cgctgagtaa gtcggaggag taatacaatg gcagttgtta
     2701 aatgtaaacc gacatctccg ggtcgtcgcc acgtagttaa agtggttaac cctgagctgc
     2761 acaagggcaa accttttgct ccgttgctgg aaaaaaacag caaatccggt ggtcgtaaca
     2821 acaatggccg tatcaccact cgtcatatcg gtggtggcca caagcaggct taccgtattg
     2881 ttgacttcaa acgcaacaaa gacggtatcc cggcagttgt tgaacgtctt gagtacgatc
     2941 cgaaccgttc cgcgaacatc gcgctggttc tgtacaaaga cggtgaacgc cgttacatcc
     3001 tggcccctaa aggcctgaaa gctggcgacc agattcagtc tggcgttgat gctgcaatca
     3061 aaccaggtaa caccctgccg atgcgcaaca tcccggttgg ttctactgtt cataacgtag
     3121 aaatgaaacc aggtaaaggc ggtcagctgg cacgttccgc tggtacttac gttcagatcg
     3181 ttgctcgtga tggtgcttat gtcaccctgc gtctgcgttc tggtgaaatg cgtaaagtag
     3241 aagcagactg ccgtgcaact ctgggcgaag ttggcaatgc tgagcatatg ctgcgcgttc
     3301 tgggtaaagc aggtgctgca cgctggcgtg gtgttcgtcc gaccgttcgc ggtaccgcga
     3361 tgaacccggt agaccaccca catggtggtg gtgaaggtcg taactttggt aagcacccgg
     3421 taactccgtg gggcgttcag accaaaggta agaagacccg cagcaacaag cgtactgata
     3481 aattcatcgt acgtcgccgt agcaaataat tttagaggat aagccatgcc acgttctctc
     3541 aagaaaggtc cttttattga cctgcacttg ctgaagaagg tagagaaagc ggtggaaagc
     3601 ggagacaaga agcccctgcg cacttggtcc cgtcgttcaa cgatctttcc taacatgatc
     3661 ggtttgacca tcgctgtcca taatggtcgt cagcacgttc cggtatttgt aaccgacgaa
     3721 atggtcggtc acaaactggg tgaattcgca ccgactcgta cttatcgcgg ccacgctgct
     3781 gataaaaaag cgaagaagaa ataaggtagg aggaagagat ggaaactatc gctaaacatc
     3841 gccatgctcg ttcttctgct cagaaggttc gccttgttgc tgacctgatt cgcggtaaga
     3901 aagtgtcgca ggctctggat attttgacct acaccaacaa gaaagcggct gtactggtca
     3961 agaaagttct ggaatctgcc attgctaacg ctgaacacaa cgatggcgct gacattgacg
     4021 atctgaaagt tacgaaaatt ttcgtagacg aaggcccgag catgaagcgc attatgccgc
     4081 gtgcaaaagg tcgtgcagat cgcatcctga agcgcaccag ccacatcact gtggttgtgt
     4141 ccgatcgctg agactctgga gactagcaat gggtcagaaa gtacatccta atggtattcg
     4201 cctgggtatt gtaaaaccat ggaactctac ctggtttgcg aacaccaaag aattcgctga
     4261 caacctggac agcgatttta aagtacgtca gtacctgact aaggaactgg ctaaagcgtc
     4321 cgtatctcgt atcgttatcg agcgtccggc taagagcatc cgtgtaacca ttcacactgc
     4381 tcgcccgggt atcgttatcg gtaaaaaagg tgaagacgta gaaaaactgc gtaaggtcgt
     4441 agcggacatc gctggcgttc ctgcacagat caacatcgcc gaagttcgta agcctgaact
     4501 ggacgcaaaa ctggttgctg acagcatcac ttctcagctg gaacgtcgcg ttatgttccg
     4561 tcgtgctatg aagcgtgctg tacagaacgc aatgcgtctg ggcgctaaag gtattaaagt
     4621 tgaagttagc ggccgtctgg gcggcgcgga aatcgcacgt accgaatggt accgcgaagg
     4681 tcgcgtaccg ctgcacactc tgcgtgctga catcgactac aacacctctg aagcgcacac
     4741 cacttacggt gtaatcggcg ttaaagtgtg gatcttcaaa ggcgagatcc tgggtggtat
     4801 ggctgctgtt gaacaaccgg aaaaaccggc tgctcagcct aaaaagcagc agcgtaaagg
     4861 ccgtaaataa ggagcgtcgc tgatgttaca accaaagcgt acaaaattcc gtaaaatgca
     4921 caaaggccgt aaccgcggtc tggcgcaggg tacggatgtt agcttcggca gcttcggtct
     4981 gaaagctgtt ggccgtggtc gtctgactgc ccgtcagatc gaagcagcac gtcgtgctat
     5041 gacccgtgca gttaagcgtc aaggtaagat ctggatccgt gtgttcccgg acaaaccgat
     5101 cactgaaaag ccgctggcag tgcgtatggg taaaggtaaa ggtaacgtgg agtattgggt
     5161 tgccttgatt cagccgggta aagtcctgta tgaaatggac ggtgttccgg aagagctggc
     5221 ccgtgaagca ttcaagctgg cagcagcgaa actgccgatt aaaaccacct ttgtaactaa
     5281 gacggtgatg taatgaaagc aaaagagctg cgtgagaaga gcgttgaaga gctgaacacc
     5341 gagctgctga acctgctgcg tgagcagttc aacctgcgta tgcaggctgc aagtggccag
     5401 ctgcaacagt ctcacctgtt gaagcaagtg cgtcgcgatg tcgcacgcgt taagacttta
     5461 ctgaacgaga aggctggtgc gtaatgaccg ataaaatccg tactctgcaa ggtcgcgttg
     5521 ttagcgacaa aatggagaaa tccattgttg ttgctatcga acgttttgtg aaacacccga
     5581 tctacggtaa attcatcaag cgtacgacca aactgcacgt acatgacgag aacaacgaat
     5641 gcggtatcgg tgacgtggtt gaaatccgcg aatgccgtcc gctgtccaag actaaatcct
     5701 ggacgctggt tcgcgttgta gagaaagcgg ttctgtaata cagtacactc tctcgatacg
     5761 aataaacggc tcagaaatga gccgtttatt ttttctaccc atatccttga agcggtgtta
     5821 taatgccgcg ccctcgatat ggggattttt aacgacctga ttttcgggtc tcagtagtag
     5881 ttgacattag cggagcacta aaatgatcca agaacagact atgctgaacg tcgccgacaa
     5941 ctccggtgca cgtcgcgtaa tgtgtatcaa ggttctgggt ggctcgcacc gtcgctacgc
     6001 aggcgtaggc gacatcatca agatcaccat caaagaagca attccgcgtg gtaaggtcaa
     6061 aaaaggtgat gtgctgaagg cggtagtggt gcgcaccaag aagggtgttc gtcgcccgga
     6121 cggttctgtc attcgcttcg atggtaatgc ttgtgttctt ctgaacaaca acagcgagca
     6181 gcctatcggt acgcgtattt ttgggccggt aactcgtgag cttcgtagtg agaagttcat
     6241 gaaaattatc tctctggcac cagaagtact ctaaggagcg aatcatggca gcgaaaatcc
     6301 gtcgtgatga cgaagttatc gtgttaaccg gtaaagataa aggtaaacgc ggtaaagtta
     6361 agaatgtcct gtcttccggc aaggtcattg ttgaaggtat caacctggtt aagaaacatc
     6421 agaagccggt tccggccctg aaccaaccgg gtggcatcgt tgaaaaagaa gccgctattc
     6481 aggtttccaa cgtagcaatc ttcaatgcgg caaccggcaa ggctgaccgt gtaggcttta
     6541 gattcgaaga cggcaaaaaa gtccgtttct tcaagtctaa cagcgaaact atcaagtaat
     6601 ttggagtagt acgatggcga aactgcatga ttactacaaa gacgaagtag ttaaaaaact
     6661 catgactgag tttaactaca attctgtcat gcaagtccct cgggtcgaga agatcaccct
     6721 gaacatgggt gttggtgaag cgatcgctga caaaaaactg ctggataacg cagcagcaga
     6781 cctggcagca atctccggtc aaaaaccgct gatcaccaaa gcacgcaaat ctgttgcagg
     6841 cttcaaaatc cgtcagggct atccgatcgg ctgtaaagta actctgcgtg gcgaacgcat
     6901 gtgggagttc tttgagcgcc tgatcactat tgctgtacct cgtatccgtg acttccgtgg
     6961 cctgtccgct aagtctttcg acggtcgtgg taactacagc atgggtgtcc gtgagcagat
     7021 catcttccca gaaatcgact acgataaagt cgaccgcgtt cgtggtttgg atattaccat
     7081 taccactact gcgaaatctg acgaagaagg ccgcgctctg ctggctgcct ttgacttccc
     7141 gttccgcaag taaggtaggg ttactaaatg gctaagcaat caatgaaagc acgcgaagta
     7201 aaacgcgtag ctttagctga taaatacttc gcgaaacgcg ctgaactgaa agcgatcatc
     7261 tctgatgtga acgcttccga cgaagatcgt tggaacgctg ttctcaagct gcagactctg
     7321 ccgcgtgatt ccagcccgtc tcgtcagcgt aaccgctgcc gtcaaacagg tcgtccgcat
     7381 ggtttcctgc ggaagttcgg gttgagccgt attaaggtcc gtgaagccgc tatgcgcggt
     7441 gaaatcccgg gtctgaaaaa ggctagctgg taattgtcac caattgaatc acgggagtaa
     7501 agacagatga gcatgcaaga tccgatcgcg gatatgctga cccgtatccg taacggtcag
     7561 gccgcgaaca aagctgcggt caccatgcct tcctccaagc tgaaagtggc aatcgccaac
     7621 gtgctgaagg aagaaggttt tattgaagat tttaaagttg aaggcgacac caagcctgaa
     7681 ctggaactta ctctgaagta tttccagggc aaagctgttg tagaaagcat tcagcgtgtc
     7741 agccgcccag gtctgcgcat ctataaacgt aaagatgagc tgccgaaagt tatggcgggt
     7801 ctgggtatcg cagttgtttc tacctctaaa ggtgttatga ctgatcgtgc agcgcgccag
     7861 gctggtcttg gtggcgaaat tatctgctac gtagcctaat cggaggaaaa aatgtctcgt
     7921 gttgctaaag caccggtcgt tgttcctgcc ggcgttgacg taaaaatcaa cggtcaggtt
     7981 attacgatca aaggtaaaaa cggcgagctg actcgtactc tcaacgatgc tgttgaagtt
     8041 aaacatgcag ataataccct gaccttcggt ccgcgtgatg gttacgcaga cggttgggca
     8101 caggctggta ccgcgcgtgc cctgctgaac tcaatggtta tcggtgttac cgaaggcttc
     8161 actaagaagc tgcagctggt tggtgtaggt taccgtgcag cggttaaagg taatgtgatt
     8221 aacctgtctc tgggtttctc tcatcctgtt gaccatcagc tgcctgcggg tatcactgct
     8281 gaatgtccga ctcagactga aatcgtgctg aaaggcgctg ataagcaggt gatcggccag
     8341 gttgcagcgg atctgcgcgc ctaccgtcgt cctgagcctt ataaaggcaa gggtgttcgt
     8401 tacgccgacg aagtcgtgcg tatcaaagag gctaagaaga agtaaggtaa cactatggat
     8461 aagaaatctg ctcgtatccg tcgtgcgacc cgcgcacgcc gcaagctcca ggagctgggc
     8521 gcaactcgcc tggtggtaca tcgtaccccg cgtcacattt acgcacaggt aattgcaccg
     8581 aacggttctg aagttctggt agctgcttct actgtagaaa aagctatcgc tgaacaactg
     8641 aagtacaccg gtaacaaaga cgcggctgca gctgtgggta aagctgtcgc tgaacgcgct
     8701 ctggaaaaag gcatcaaaga tgtatccttt gaccgttccg ggttccaata tcatggtcgt
     8761 gtccaggcac tggcagatgc tgcccgtgaa gctggccttc agttctaagg tagaggtgta
     8821 agatggctca catcgaaaaa caagctggcg aactgcagga aaagctgatc gcggtaaacc
     8881 gcgtatctaa aaccgttaaa ggtggtcgta ttttctcctt cacagctctg actgtagttg
     8941 gcgatggtaa cggtcgcgtt ggttttggtt acggtaaagc gcgtgaagtt ccagcagcga
     9001 tccagaaagc gatggaaaaa gcccgtcgca atatgattaa cgtcgcgctg aataacggca
     9061 ctctgcaaca ccctgttaaa ggtgttcaca cgggttctcg cgtattcatg cagccggctt
     9121 ccgaaggtac cggtatcatc gccggtggtg caatgcgcgc cgttctggaa gtcgctgggg
     9181 ttcataacgt tctggctaaa gcatatggtt ccaccaaccc gatcaacgtg gttcgtgcaa
     9241 ctattgatgg cctggaaaat atgaactctc cagaaatggt cgctgccaag cgtggtaaat
     9301 ccgttgaaga aattctgggg aaataaacca tggcaaagac tattaaaatt actcaaaccc
     9361 gcagtgcaat cggtcgtctg ccgaaacaca aggcaacgct gcttggcctg ggtctgcgtc
     9421 gtattggtca caccgtagag cgcgaggata ctcctgctat tcgcggtatg atcaacgcgg
     9481 tttccttcat ggttaaagtt gaggagtaag agatgcgttt aaatactctg tctccggccg
     9541 aaggctccaa aaaggcgggt aaacgcttgg gtcgtggtat cggttctggc ctcggtaaaa
     9601 ccggtggtcg tggtcacaaa ggtcagaagt ctcgttctgg cggtggcgta cgtcgcggtt
     9661 tcgagggtgg tcagatgcct ctgtaccgtc gtctgccgaa attcggcttc acttctcgta
     9721 aagcagcgat tacagccgaa gttcgtctgt ctgacctggc taaagtagaa ggcggtgttg
     9781 tagacctgaa cacgctgaaa gcggctaaca ttatcggtat ccagatcgag ttcgcgaaag
     9841 tgatcctggc tggcgaagta acgactccgg taactgttcg tggcctgcgt gttactaaag
     9901 gcgctcgtgc tgctatcgaa gctgctggcg gtaaaatcga ggaataagta gcagatggct
     9961 aaacaaccgg gattagattt tcaaagtgcc aaaggtggct taggcgagct gaaacgcaga
    10021 ctgctgtttg ttatcggtgc gctgattgtg ttccgtattg gctcttttat tccgatccct
    10081 ggtattgatg ccgctgtact tgccaaactg cttgagcaac agcgaggcac catcattgag
    10141 atgtttaaca tgttctctgg tggtgctctc agccgtgctt ctatctttgc tctggggatc
    10201 atgccgtata tttcggcgtc gatcattatc cagctgctga cggtggttca cccaacgttg
    10261 gcggaaatta agaaagaagg ggagtctggt cgtcgtaaga tcagccagta cacccgctac
    10321 ggtactctgg tgctggcaat attccagtcg atcggtattg ctaccggtct gccgaatatg
    10381 cctggtatgc aaggcctggt gattaacccg ggctttgcat tctacttcac cgctgttgta
    10441 agtctggtca caggaacgat gttcctgatg tggttgggcg aacagatcac tgaacgaggt
    10501 atcggcaacg gtatttcaat cattatcttc gccggtattg tcgcgggact cccgccagcc
    10561 attgcccata ctatcgagca agcgcgtcaa ggcgacctgc acttcctcgt gttgctgttg
    10621 gttgcagtat tagtatttgc agtgacgttc tttgttgtat ttgttgagcg tggtcaacgc
    10681 cgcattgtgg taaactacgc gaaacgtcag caaggtcgtc gtgtctatgc tgcacagagc
    10741 acacatttac cgctgaaagt gaatatggcg ggggtaatcc cggcaatctt cgcttccagt
    10801 attattctgt tcccggcgac catcgcgtca tggttcgggg gcggtactgg ttggaactgg
    10861 ctgacaacaa tttcgctgta tttgcagcct gggcaaccgc tttatgtgtt actctatgcg
    10921 tctgcaatca tcttcttctg tttcttctac acggcgttgg ttttcaaccc gcgtgaaaca
    10981 gcagataacc tgaagaagtc cggtgcattt gtaccaggaa ttcgtccggg agagcaaacg
    11041 gcgaagtata tcgataaagt aatgacccgc ctgaccctgg ttggtgcgct gtatattacc
    11101 tttatctgcc tgatcccgga gttcatgcgt gatgcaatga aagtaccgtt ctacttcggt
    11161 gggacctcac tgcttatcgt tgttgtcgtg attatggact ttatggctca agtgcaaact
    11221 ctgatgatgt ccagtcagta tgagtctgca ttgaagaagg cgaacctgaa aggctacggc
    11281 cgataattgg tcgcccgaga agttacggag agtaaaaatg aaagttcgtg cttccgtcaa
    11341 gaaattatgc cgtaactgca aaatcgttaa gcgtgatggt gtcatccgtg tgatttgcag
    11401 tgccgagccg aagcataaac agcgccaagg ctgatttttt cgcatatttt tcttgcaaag
    11461 ttgggttgag ctggctagat tagccagcca atcttttgta tgtctgtgcg tttccatttg
    11521 agtatcctga aaacgggctt ttcagcatgg aacgtacata ttaaatagta ggagtgcata
    11581 gtggcccgta tagcaggcat taacattcct gatcataagc atgccgtaat cgcattaact
    11641 tcgatttatg gcgtcggcaa gacccgttct aaagccatcc tggctgcagc gggtatcgct
    11701 gaagatgtta agatcagtga gctgtctgaa ggacaaatcg acacgctgcg tgacgaagtt
    11761 gccaaatttg tcgttgaagg tgatctgcgc cgtgaaatca gcatgagcat caagcgcctg
    11821 atggatcttg gttgctatcg cggcttgcgt catcgtcgtg gtctcccggt tcgcggtcag
    11881 cgtaccaaga ccaacgcacg tacccgtaag ggtccgcgca aaccgatcaa gaaataatcg
    11941 gggtgattga ataatggcaa aggcaccaat tcgtgcacgt aaacgtgtaa gaaaacaagt
    12001 ctctgacggc gtggctcata tccatgcttc tttcaacaac accatcgtga ctatcactga
    12061 tcgtcagggt aacgcgttgg gttgggcaac agccggtggt tccggtttcc gtggttctcg
    12121 caaatccact ccgtttgcag ctcaggttgc agcagagcgt tgcgctgacg ccgtgaaaga
    12181 atacggcatc aagaatctgg aagttatggt taaaggtccg ggtccaggcc gcgaatctac
    12241 tattcgtgct ctgaacgccg caggtttccg catcactaac attactgatg tgactccgat
    12301 ccctcataac ggttgtcgtc cgccgaaaaa acgtcgcgta taacgcctcg ttttccaggt
    12361 ttgttggaga aagaaaatgg caagatattt gggtcctaag ctcaagctga gccgtcgtga
    12421 gggcaccgac ttattcctta agtctggcgt tcgcgcgatc gataccaagt gtaaaattga
    12481 acaagctcct ggccagcacg gtgcgcgtaa accgcgtctg tctgactatg gtgtgcagtt
    12541 gcgtgaaaag caaaaagttc gccgtatcta tggtgtgctg gagcgtcagt tccgtaacta
    12601 ctacaaagaa gcagcacgtc tgaaaggcaa caccggtgaa aacctgttgg ctctgctgga
    12661 aggtcgtctg gacaacgttg tataccgtat gggcttcggt gccactcgtg cagaagcacg
    12721 tcagctggtt agccataaag caattatggt aaacggtcgt gttgttaata tcgcttctta
    12781 tcaggttagt ccgaatgacg ttgtaagcat tcgtgagaaa gcgaagaagc agtctcgcgt
    12841 gaaagccgct ctggagctgg ctgagcagcg tgaaaagcca acttggctgg aagttgatgc
    12901 tggcaagatg gaaggtacgt ttaagcgtaa gccggagcgt tctgatctgt ctgcggacat
    12961 taacgaacac ctgatcgtcg agctttactc caagtaaagc ttagtaccaa agagaggaca
    13021 caatgcaggg ttctgtgaca gagtttctaa aaccgcgcct ggttgatatc gagcaagtga
    13081 gttcgacgca cgccaaggtg acccttgagc ctttagagcg tggctttggc catactctgg
    13141 gtaacgcact gcgccgtatt ctgctctcat cgatgccggg ttgcgcggtg accgaggttg
    13201 agattgatgg tgtactacat gagtacagca ccaaagaagg cgttcaggaa gatatcctgg
    13261 aaatcctgct caacctgaaa gggctggcgg tgagagttca gggcaaagat gaagttattc
    13321 ttaccttgaa taaatctggc attggccctg tgactgcagc cgatatcacc cacgacggtg
    13381 atgtcgaaat cgtcaagccg cagcacgtga tctgccacct gaccgatgag aacgcgtcta
    13441 ttagcatgcg tatcaaagtt cagcgcggtc gtggttatgt gccggcttct acccgaattc
    13501 attcggaaga agatgagcgc ccaatcggcc gtctgctggt cgacgcatgc tacagccctg
    13561 tggagcgtat tgcctacaat gttgaagcag cgcgtgtaga acagcgtacc gacctggaca
    13621 agctggtcat cgaaatggaa accaacggca caatcgatcc tgaagaggcg attcgtcgtg
    13681 cggcaaccat tctggctgaa caactggaag ctttcgttga cttacgtgat gtacgtcagc
    13741 ctgaagtgaa agaagagaaa ccagagttcg atccgatcct gctgcgccct gttgacgatc
    13801 tggaattgac tgtccgctct gctaactgcc ttaaagcaga agctatccac tatatcggtg
    13861 atctggtaca acgtaccgag gttgagctcc ttaaaacgcc taaccttggt aaaaaatctc
    13921 ttactgagat taaagacgtg ctggcttccc gtggactgtc tctgggcatg cgcctggaaa
    13981 actggccacc ggcaagcatc gctgacgagt aaccggatca caggttaagg ttttactgag
    14041 aaggataagg tcatgcgcca tcgtaagagt ggtcgtcaac tgaaccgcaa cagcagccat
    14101 cgccaggcta tgttccgcaa tatggcaggt tcactggttc gtcatgaaat catcaagacg
    14161 actctgccta aagcgaaaga gctgcgccgc gtagttgagc cgctgattac tcttgccaag
    14221 actgatagcg ttgctaatcg tcgtctggca ttcgcccgta ctcgtgataa cgagatcgtg
    14281 gcaaaactgt ttaacgaact gggcccgcgt ttcgcgagcc gtgccggtgg ttacactcgt
    14341 attctgaagt gtggcttccg tgcaggcgac aacgcgccga tggcttacat cgagctggtt
    14401 gatcgttcag agaaagcaga agctgctgca gagtaatctg aagcaacgta aaaaaacccg
    14461 ccccggcggg tttttttata cccgtagcat ccccacttat ctacaatagc tgtactcttt
    14521 ttgttcatcc cctggagtat ttatgtggtt acttgaccag tgggcagagc gtcatatagc
    14581 agaagcgcaa gcgaaaggtg agtttgataa tctggcaggt agcggcgaac cattgatact
    14641 ggatgatgat tctcatgtgc caccggaatt acgtgcgggg tatcgcttgc tgaagaatgc
    14701 tggttgctta ccgccagaac ttgagcagcg gagggaagca attcagcttc tggatatcct
    14761 gagaggtatc cgtcacgatg atccgcaata tcaagaggtt agccgtcgat tgtcattact
    14821 ggaattgaag ctgcgacaag ctggatttag taccgatttt ttacgcggcg attatgctga
    14881 caagttgttg gacaaaatca acgataacta gtggagtatg tatgtatcgc attggtgagc
    14941 tggcaaaaat ggcggaagta acacctgaca cgattcgtta ttacgaaaaa cagcagatga
    15001 tggagcatga agtgcgtact gaaggcgggt ttcgcctgta taccgaaagc gatctccagc
    15061 gattgaaatt tatccgccat gccagacaac taggtttcag tctggagtcg atccgcgagt
    15121 tgctgtcgat ccgcatcgat cctgaacacc atacctgtca ggagtcaaaa ggcattgtgc
    15181 aggaaagatt gcaggaagtc gaagcacgga tagccgagtt gcagagtatg cagcgttcct
    15241 tgcaacgcct taacgatgcc tgttgtggga ccgcccatag cagtgtttat tgttcgattc
    15301 ttgaagctct tgaacaaggg gcgagtggcg ttaagagtgg ctgttgattt tttgcactgg
    15361 caggattaca ctcgcgccgt aaaataacca attggagttt ttatgagccg atatcagcat
    15421 actaaagggc agataaggga taatgcgata gaagcattac tacatgatcc cttattccga
    15481 cagcgcgtag agaaaaataa gaaggggaaa ggcagttaca tgcgaaaagg aaaacatggc
    15541 aatcggggga actgggaggc cagtggcaag aaagtaaatc acttttttac cactggtctt
    15601 ctgctttcag gcgcttgtta agagcggtta ttctgctctt tcagcaaatc acgaatttct
    15661 gccagtaata cttcttcttt agttggtgca ggtgcggcag ctggttcttc ttttttccga
    15721 ttcagtttgt tgattagctt aatcgccata aagatggcaa aggccacaat cagaaaatca
    15781 aagacgtttt gaatgaagac accgtaatgc atcacaacag cagggatatc cccctgcgca
    15841 tcgcgtagcg tgacagcaaa ctgtttaaag tcgatcccgc caattaataa gcccagaggt
    15901 ggcatgatga tatcggcaac cagtgaagat acaatcttcc cgaatgccgc accgataatg
    15961 acacccaccg ccaaatccac cacgttcccg cgcatcgcaa attcgcgaaa ttctttaata
    16021 atgctcatgt tattctccct atgaagccga caaccataag tctaacaaat gttaagccat
    16081 tttcctgcca ggaatgaatt taattaatcc ttgataatca aagggaaata ataaggcgtc
    16141 attagacgcc ttattaatta caagaagaaa gggcttggct ggaagagtct ttcgacgtcg
    16201 gtaataaact ttttatctgt gaggaacata attacgtgat cgccttgctc aatgcgcaga
    16261 ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg ctccaataat cgttcctggc
    16321 ggtagcttga tttcgtcaat gactctgccg acaacgcgtg aggtgctttc atcaccgtga
    16381 gcaacggctt caatagcttc tgctacgccg cggcgcaatg aggaaacacc aacaatatct
    16441 gcttttcgca catggctaag caacgcagaa atagttgctt gttgtggtga aattgcaata
    16501 tcgatcacgc tcccctgaac cagatccaca taagcgcgac gctggatcaa taccatcacc
    16561 tttttcgcac ccatacgttt ggcaagcatg gcggacatga tattggcctc gtcatcgttg
    16621 gtgacggcaa taaacagatc aacttgatcg atatgttctt cggccagcag ttcttgatcc
    16681 gacgcatcac caaaaaagac gatcgtattc tgcaactttt ccgccagttc ggcagcgcgc
    16741 tgctgattac gttcgatgag tttaacgctg taatcttttt ccagacgacg cgccagccct
    16801 gcaccgatat taccgccacc aaccagcatg atccgcttat acggtttttc cagtcgctgt
    16861 aattcactca tcaccgcgcg gatatgctgt gaagcggcaa taaagaacac ttcatcacca
    16921 gcttcaacaa tggtcgaacc ttgtggacga atggggcgat cgtggcggaa aattgctgcc
    16981 acacgagtat cgatatgtgg catatgttcg cgcatggtcg aaagtgcatt accaatcagc
    17041 gggccgccat aataggcttt aactacagcg aggctgactt taccctcagc gaagttcacc
    17101 acctgcaatg cgcctggata ctcaatcagt cggtaaatat tatcgataac caactgctct
    17161 ggtgcgatca gatgatcaat cggtacagca tctgaatgaa atagcttatc ggcatcgcgt
    17221 acgtagtctg gtgagcggat gcgagcgatg cgattagggg tgttgaaaag tgagtaggct
    17281 acctggcagg caaccatatt ggtttcatct gaactggtta cagcaaccag catatcggcg
    17341 tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc catgcccctg cacgactcgc
    17401 aggtcaaatt tatcctgcaa ggtccgcaga cgctcaccgt tggtatcgac aacagtaata
    17461 tcgttgttct cgccaaccag gttttccgcc agtgtgccgc caacctggcc ggcacccaga
    17521 atgataattt tcatcagtct cgacccgtta tctcatcact ttttgattag cttagcgtaa
    17581 aagaagccgt cgccctcttc ggcaccaggc agattttgtt tacccggttg ctctggtgtt
    17641 cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa aagctttaat ctgcaggcta
    17701 ttctcttccg gtaacaccga acaggtggca tagaccaggg ttccaccgga ttttaaatgc
    17761 ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga gttccgggat atcgcgatcg
    17821 cgacgtaacc atttaatatc cggatggtga cgaatcaccc ccgttgctga acaaggcgca
    17881 tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt gggaagggta acggccatca
    17941 ccttgtttca cggttgcctt cataccaagg cgttttaaat tgtcgtaaac gcgagagagg
    18001 cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg gtgccacctc aaggatatgc
    18061 gttgttttac cgccgggggc ggcacaaaga tccagaatgt gttcaccgtt ttgtggcgca
    18121 agccaggtca tgcaaccttg tgctgatgca tcctgaacgg taacccatcc gtcttcaaaa
    18181 ccaggtagcg catgaacagg tgcaggtgtt tccagacgta cagcatcagg gtaatccgca
    18241 tgcgggaaac ctttcattcc tgcttcatcc agcaatgcaa gccagctgtc gcgggaatga
    18301 tgcgtacgat taacgcgcag ccacattggc ggacgctggt tattggcttc gacgatggat
    18361 tgccactgct ctggatacgc tttttgcaga cgcttcagca accaggaagg atgcagataa
    18421 cgtgcatcac tggcattaaa cttggctaat aactcttctt gctgacgctg gaactggcgt
    18481 aatacaccgt taatcaaccc tttaagttgc ggacgcttaa ttgcgatagc gccttcaacc
    18541 gtttcagcca gcgcagcatg aggtggaatg cgggtataaa gcagttgata caaaccaacc
    18601 ataatcaggt aatgcacagt gcgctgtttg ccggtcatcg gacgggccat taacttatta
    18661 atcagccagt ctaactgcga aagcgtacgc agtacgccaa agcacaactc ttgaagaagt
    18721 gctttgtctt tatcagaaac tttttgctgg agcggtggca gaatgttgct taatgattgc
    18781 ccttgctcga cgacttgttc aacggcctgg gccgccatgc tacgtaaatt acgttgtttt
    18841 ttcataagta taaaaatgcc cggtaagacc gggcttagaa gagtggacta tcaggccaga
    18901 cggttgcccg gaacaaacca ttcccgacga gaattcagga ggtcttgcgc gctcatcgct
    18961 ttcttacccg caggttgtaa cgagagcaga ttcaggattc catcaccagt tgcaacctga
    19021 atgccttgtt tgttggcttc aaggatcgtt cctggtgcag cgttggttgc cgtatcaatg
    19081 accgacgctt tccagacttt aaccggctgt ctttcaattt ccagccagct cattggccat
    19141 ggattgaaag cgcgaatgta gcgttcaagc tgtgctgccg aaagtgacca gtcaatacgc
    19201 gcttcttctt tactcaactt ttcggcgtaa gtgacaagag tttcgtcctg aacttctggt
    19261 ttcgccgtgc cgtctgccag ttgtttcaac gtggtgataa gcccttgtgg gccaagctct
    19321 gccagcttgt cgtacagcgt accactggta tcttctgcag taatcgggca ggagagctta
    19381 tagagcatgt caccggtgtc taaaccgaca tccatttgca taatggtcac accagtttct
    19441 gcatcacccg cccatagtga gcgttggatt ggtgcagcac cgcgccagcg tggcagcagt
    19501 gaaccatgaa cgttgataca gccaagacgc ggcatctcca gcactgcttt aggcagaatt
    19561 aagccatagg cgacgacgac cataacatca gcctgcagat cggcgaccag ttgctggttt
    19621 tcttgtggac gcagggaaac aggttgaaaa acgggcagac ctttttcctc agccagaact
    19681 ttaaccgggc tgggcatcag ttttttaccg cgtcctgccg gtcggtctgg ctgggtgaac
    19741 acgccaacga cgttatgacc agaagacaac agcgcgtcga gatgacgcgc tgcaaagtca
    19801 ggtgtacccg caaaaataat acgtagtgat tctgacacgt tagttcttat ccttaagccc
    19861 gggctttcag acgatccagt ttttcaactt tctgacgaat acgttgttgt ttcaacggtg
    19921 acagataatc cataaacagt ttgccgacca ggtgatccat ctcatgctga atacagatgg
    19981 ctaacagacc gtctgcttcc aattcaaatg gtttaccgtc gcggtccagg gcgcgaattt
    20041 taactttctc tgcgcgtggc actaaagcac gttgttcagg gatcgacagg caaccttctt
    20101 caatgcctgt ttcgccgctt ttttctaaaa gctccggatt gattaacacc agccgttcgt
    20161 cacggttttc cgaaacatca ataacaatga tacgttgatg gatatcaacc tgggttgccg
    20221 ccaggccaat accttcttct gcgtacatcg tctcgaacat atcatcgacg atacgctgaa
    20281 tttctgcatt cacttcttct accggtttag caactttgcg aagccgctcg tccggaatat
    20341 gtaacacttg caaaactgac ataaatctcc agagatgtgt tcaggagtta gaaagattat
    20401 ttcttctatt ctagacaaat ccccctctga ttgacagcat cactgaccaa tcgcaaagat
    20461 tgctaaggct gcttatggca gggagataag gatggtcgat acagatattt ggctgcgttt
    20521 aatgagcatc agcagcttgt acggcgatga tatggtccgt atagctcact ggctggcaaa
    20581 acagtcgcat attgatgcgg ttgtattgca gcaaacaggg cttacattgc ggcaggcaca
    20641 acgctttctt tcatttccgc gaaagagtat cgaaagctca ctttgttggt tggagcaacc
    20701 caaccatcat ttaatccctg cggacagcga attttatcct cctcaacttc tggcgacgac
    20761 agattacccc ggcgcactgt ttgttgaagg agaactgtac gcgctgcatt catttcagct
    20821 tgccgtagtg gggagtcggg cgcattcatg gtatggcgag cgatggggac gattattttg
    20881 cgaaactctg gccacgcgtg gagtgacaat tacgagtgga ctggcgcgtg gaatcgatgg
    20941 tgtggcgcat aaagcggcct tacaggtaaa tggcgtcagc attgctgtat tggggaacgg
    21001 acttaatacc attcatccgc gccgccatgc ccgactggct accagtttgc ttgaacatgg
    21061 tggggcactt gtctcggaat ttcccctcga tgtttcaccc cttgcttaca atttcccacg
    21121 aagaaatcgc attatcagtg gtctaagtaa aggtgtactg gtggtggaag cggctttgcg
    21181 cagtggttcg ttggtgacag cacgttgtgc gcttgagcag gggcgtgaag tttttgcctt
    21241 gccaggacca atagggaatc cgggaagcga agggcctcac tggttaataa aacaaggtgc
    21301 gattcttgtg acggaaccgg aagaaattct ggaaaacttg caatttggat tgcactggtt
    21361 gccagacgcc cctgaaaatt cattttattc accagatcag caagacgtgg cattgccatt
    21421 tcctgagctc ctggctaacg taggagatga ggtaacacct gttgacgtcg tcgctgaacg
    21481 tgccggccaa cctgtgccag aggtagttac tcaactactc gaactggagt tagcaggatg
    21541 gatcgcagct gtacccggcg gctatgtccg attgaggagg gcatgccatg ttcgacgtac
    21601 taatgtattt gtttgaaacc tatattcaca cagaagctga gttgcgtgtg gatcaagaca
    21661 aacttgaaca ggatcttacc gacgcaggat ttgatcgaga agatatctac aatgccctgc
    21721 tttggctgga aaagcttgcg gattatcagg aaggactgac agaaccgatg caactggcct
    21781 ctgatccact ctccatgcgt atttataccc ctgaggagtg tgaaagactg gatgccagct
    21841 gtcgtggttt tctgcttttc cttgagcaaa ttcaggtgct taaccttgaa atccgtgaaa
    21901 tggtgataga gcgagtgctc gcgctggata ccgcagagtt cgacctggaa gacctgaaat
    21961 gggtaatcct gatggtgttg ttcaatattc cgggctgcga aaatgcgtac cagcaaatgg
    22021 aagaattact ctttgaagtg aatgaaggta tgctgcatta atcatttctt taattcagca
    22081 taagttgtta tggcgaaatc agcactgttc acggtgcgta ataatgagtc ctgcccaaag
    22141 tgcggggctg aactggttat tcgatccggg aaacacggtc cgtttcttgg atgctcacag
    22201 tatccggcgt gtgactacgt ccgtcctctg aaatcttcag cggatggaca tattgtcaaa
    22261 gttctggagg ggcaggtttg ccctgcatgt ggcgcaaatc tggtattacg ccagggacgc
    22321 tttggtatgt ttattggttg cagtaactac cctgaatgcg aacataccga acttatcgat
    22381 aaaccggacg aaacagcaat tacatgcccc caatgtcgga cgggccatct ggtccagcgc
    22441 cgctcccgtt atggcaaaac atttcactcc tgtgatcgct acccggagtg tcaatttgcc
    22501 attaacttca aacccatagc tggagaatgc cctgagtgtc attatccact actcatcgaa
    22561 aagaaaaccg cgcagggtgt aaaacacttt tgtgccagta aacaatgtgg aaagccggtt
    22621 tcggcggaat aataacgtga ataataacct gcaaggagac gctatcgcag ctgcgataga
    22681 tgtcctcaat gaagaacgtg tcatcgccta tccaacggaa gccgttttcg gtgttgggtg
    22741 cgatcctgat agcgaaacag cagtgatgag actgttggag ttaaaacagc gtccggttga
    22801 taaggggctg attttaatcg cggcaaatta cgagcagctt aaaccctata ttgatgacac
    22861 catgctgact gatgcgcagc gtgaaaccat tttttcccgc tggccaggtc ctgtcacctt
    22921 tgtctttccc gcgcctgcga caacaccgcg ctggttgacg ggccgctttg attcgcttgc
    22981 tgtacgagtc accgaccatc cgttggtggt tgctttgtgc caggcttatg gtaaaccgct
    23041 ggtttctacc agtgccaact tgagtggatt gccaccttgt cgaacagtag acgaagttcg
    23101 cgcacaattt ggcgcggcgt tcccggttgt gcttggtgaa acgggggggc gtttaaatcc
    23161 ttcagaaatc cgcgatgccc tgacgggtga actgtttcga caggggtaac ataatggaaa
    23221 cctatgctgt ttttggtaat ccgatagccc acagcaaatc gccattcatt catcagcaat
    23281 ttgctcagca actgaatatt gaacatccct atgggcgcgt gttggcaccc atcaatgatt
    23341 tcatcaacac actgaacgct ttctttagtg ctggtggtaa aggtgcgaat gtgacggtac
    23401 cttttaaaga agaggctttt gccagagcgg atgagcttac tgaacgggca gcgttggctg
    23461 gtgctgttaa taccctcaag cggttagaag atggacgctt gcagggtgac aataccgatg
    23521 gtataggttt gttaagcgat ctggaacgtc tgtcttttat ccgtccgggt ttacgtattc
    23581 tgcttatcgg cgctggtgga gcatctcgcg gcgtactact accactcctt tccctggact
    23641 gtgcggtgac aatcactaat cggacggtat cccgtgcgga agagttggct aaattgtttg
    23701 cgcacactgg cagtattcag gcgttgggta tggacgaact ggaaggtcat gagtttgatc
    23761 tcattattaa tgcaacatcc agtggcatca gtggtgatat tccggcgatc ccgtcatcgc
    23821 tcattcatct aggcatttat tgctatgaca tgttctatca gaaaggaaaa actccttttc
    23881 tggcatggtg tgagcagcga ggctcaaagc gtaatgctga tggtttagga atgctggtgg
    23941 cacaggcggc tcatgccttt cttctctggc acggtgttct gcctgacgta gaaccagtta
    24001 taaagcaatt gcaggaggaa ttgtccgcgt gaatcaggcc atccagtttc cggacaggga
    24061 agagtgggac gagaataaaa aatgtgtatg ttttcccgct ctcgtgaatg gtatgcaact
    24121 gacatgcgcg atctctggcg agagtctggc gtatcgcttt actggagata cgccagaaca
    24181 gtggttagcg agttttcgtc agcatcgctg ggacctggaa gaagaagcgg aaaacttaat
    24241 tcaggaacaa agtgaagatg atcaaggctg ggtctggtta ccctgatcca gatattcgtc
    24301 cttccatttc acgtaattat tcgcggaata gcgtaaccca gccttctctt catcacttaa
    24361 cgggcggatc tgtttgacgg ggctaccgag atacagatat ccgctctcca gccgtttatt
    24421 ttgtgggacc agactacccg caccaatcat cacatcatct tctactattg cgccatcaag
    24481 taaaattgag cccatcccaa ccaaaactcg attgccaatg gtgcagccgt ggagcatcac
    24541 cttgtgacca acagtgacat cttcgccaat ggttaatggg ttgccatctg ggttgtacga
    24601 ggatttatga gtgacatgca acatactgcc atcctggata ttggtgcgtg ctccgatctg
    24661 tacataatgt acatctccac gaatcacaac gagcggccag atccccacat catcagccag
    24721 acgaacgcca ccaatcacga cactgctatc gtcgatcatt acgcgctgac cgatttgtgg
    24781 aaaaagatcg cggtatgggc gtaaaacatc agacatactt acctcagcaa taaatgattt
    24841 actaatgact ttgggggcat tattggcctt gtgcaagtct tttagtatgc aaaaaagcac
    24901 cgttttgtgt gcgattgcag caaaaagggt gaaaaaacaa caaacagaaa aaaagatcaa
    24961 aaaaatactt gtgcaaaata ttgggatccc tataatgcgc ctccgttaag acgacaacgt
    25021 gaaacacttc acaggatggt cggaacaacg aagagaaaaa atcctgaaat tcagggttga
    25081 ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca gtgagctgaa agccgcgtcg
    25141 caactgctct ttaacaattt atcagacaat ctgtgtgggc actcgaagat acggattctt
    25201 aacgtcgcaa gacgaaaaat gaataccaag tctcaagagt ga
//
LOCUS       DAJTSL010000046        25011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTSL010000046 DAJTSL010000000
VERSION     DAJTSL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.046"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..808
                     /locus_tag="OO433_001710"
     CDS             <1..808
                     /locus_tag="OO433_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR6050223.1"
                     /translation="PRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTG
                     GRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVIN
                     LTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAF
                     AMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHN
                     GFSYWIYGCMGVLAALFMWKFAPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(852..1250)
                     /gene="psiE"
                     /locus_tag="OO433_001711"
     CDS             complement(852..1250)
                     /gene="psiE"
                     /locus_tag="OO433_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6050224.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLIIVDHK
                     SPLDVLIYSVAILLLVITLWLCNSKRLKRE"
     gene            1520..1798
                     /locus_tag="OO433_001712"
     CDS             1520..1798
                     /locus_tag="OO433_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050225.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(1845..3941)
                     /locus_tag="OO433_001713"
     CDS             complement(1845..3941)
                     /locus_tag="OO433_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6050226.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIGGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNVGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     MADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVLKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3941..4678)
                     /locus_tag="OO433_001714"
                     /pseudo
     CDS             complement(3941..4678)
                     /locus_tag="OO433_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
     gene            complement(4675..5313)
                     /locus_tag="OO433_001715"
     CDS             complement(4675..5313)
                     /locus_tag="OO433_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6050227.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(5427..5669)
                     /locus_tag="OO433_001716"
     CDS             complement(5427..5669)
                     /locus_tag="OO433_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050228.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="OO433_001717"
     CDS             complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="OO433_001717"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6050229.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            8305..9654
                     /gene="lysC"
                     /locus_tag="OO433_001718"
     CDS             8305..9654
                     /gene="lysC"
                     /locus_tag="OO433_001718"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6050230.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            9906..10178
                     /locus_tag="OO433_001719"
     CDS             9906..10178
                     /locus_tag="OO433_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6050231.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="OO433_001720"
     CDS             complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="OO433_001720"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6050232.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            11531..12478
                     /locus_tag="OO433_001721"
                     /pseudo
     CDS             11531..12478
                     /locus_tag="OO433_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            12548..13354
                     /locus_tag="OO433_001722"
     CDS             12548..13354
                     /locus_tag="OO433_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6050233.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALVWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTHG"
     gene            13364..13771
                     /locus_tag="OO433_001723"
     CDS             13364..13771
                     /locus_tag="OO433_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6050234.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            13771..14265
                     /locus_tag="OO433_001724"
     CDS             13771..14265
                     /locus_tag="OO433_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6050235.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            14331..15128
                     /locus_tag="OO433_001725"
     CDS             14331..15128
                     /locus_tag="OO433_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6050236.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSAFGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            15139..15981
                     /locus_tag="OO433_001726"
     CDS             15139..15981
                     /locus_tag="OO433_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000437910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6050237.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITTALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLSRF
                     FAPA"
     gene            16015..17244
                     /gene="sorE"
                     /locus_tag="OO433_001727"
     CDS             16015..17244
                     /gene="sorE"
                     /locus_tag="OO433_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6050238.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSQLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            17627..18316
                     /gene="pepE"
                     /locus_tag="OO433_001728"
     CDS             17627..18316
                     /gene="pepE"
                     /locus_tag="OO433_001728"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6050239.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEDNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(18407..20038)
                     /locus_tag="OO433_001729"
     CDS             complement(18407..20038)
                     /locus_tag="OO433_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6050240.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTAALTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQSDEDEGRDEY"
     gene            complement(20258..23946)
                     /gene="metH"
                     /locus_tag="OO433_001730"
                     /pseudo
     CDS             complement(20258..23946)
                     /gene="metH"
                     /locus_tag="OO433_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            24146..24970
                     /gene="iclR"
                     /locus_tag="OO433_001731"
     CDS             24146..24970
                     /gene="iclR"
                     /locus_tag="OO433_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6050241.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         5883 a         6617 c         6532 g         5979 t
ORIGIN      
        1 tcctcgctgg ctgatgtcac gcggcaagca agaacaggcg gaaagtatcc tgcgcaaaat
       61 tatgggcaac acgcttgcaa ctcaggcagt acaggaaatt aaacactccc tggatcatgg
      121 ccgcaaaacc ggtggtcgtc tgctgatgtt tggcgtgggc gtgattgtca tcggcgttat
      181 gctctccatc ttccagcaat ttgtcggcat caatgtggtg ctgtactacg cgccggaagt
      241 gttcaaaacg ctgggggcca gcacggatat cgcgctgttg cagaccatta ttgtcggagt
      301 tatcaacctc accttcaccg ttctggcaat tatgacggtg gataaatttg gtcgtaagcc
      361 actgcaaatt atcggcgcac tcggaatggc aatcggtatg tttagcctcg gtaccgcgtt
      421 ttacactcag gcaccgggta ttgtggcgct actgtcgatg ctgttctatg ttgctgcctt
      481 tgccatgtcc tggggtccgg tatgctgggt actgctgtcg gaaatcttcc cgaatgctat
      541 tcgtggtaag gcgctggcaa tcgcggtggc ggcccagtgg ctggcgaact acttcgtctc
      601 ctggaccttc ccgatgatgg acaaaaactc ctggctggtg gcccatttcc acaacggttt
      661 ctcctactgg atttacgggt gtatgggcgt tctggcagca ctatttatgt ggaaatttgc
      721 cccggaaacc aaaggtaaaa cccttgagga gctggaagcc ctctgggaac cggaaacgaa
      781 gaaaacacaa caaactgcta cgctgtaatc ttcctgtcca gcacgccgcg ccagttcggc
      841 gtgctgtctt tttactcccg cttcagccgt ttcgaattac acagccacag agtgatcacc
      901 agtaacagga tcgcaaccga gtagatcaac acatccagtg gcgatttatg atcgacgatg
      961 atcaagcgca caatggcggt gatcccaata tagacaaagt aacgtagcgg gaagtgaaaa
     1021 ccggactgaa agtacttcac aatcagcgcg ataaattcga aatagagaaa gtaaaccacc
     1081 agctcatatt tgctggtttg ttctggcgcg aacagcacat ctgccagatg caccgtttct
     1141 ttgccgagga agacaaccaa aatcaggccg aggcacagca ggccaagatt gagtacggtc
     1201 tgcaaaatgg tggagataaa ctccacgcgc ggacgagaga gtgacgtcat agcagtacct
     1261 ccttcacagt ggcgagcttc ctgtataacg caaaaatgtg acggagatct atattttttg
     1321 tgtatgtttt gttcaacctg gatttggagc gcgcctccac caaagccgga tatacctacc
     1381 cgtaggccgg ataagccgcg cctggcggcg catccggcaa gacaaaccgc acccaagccg
     1441 gtaattcaaa aatcacccat tcaggtgatt cccagccacc agcaatgcgc gaaaaatcga
     1501 caatgttcct ctggaacaaa tgaaacgtaa cctgataaag gtggtaaaaa tgaagcctta
     1561 ttttgctgct ttgatgttat cagtctctgt tcttcctgct tatgctggtc cgctcggtac
     1621 tgctgataaa gccgacctac cgcaaagcaa tgtctccagc ccaatgatgg cccagtcgct
     1681 caggcaacca gacctgcaac cgatatcgac ggacaggaag acagaatgtt ttcgccttta
     1741 cacaccagat cgcaaaccgg gggtgaactg cgttcctgat ggctcgacag gccattaaga
     1801 atgaaggtgc cagtgagtgc gaaaactggc acctttccca tgacttagcg gaaattgacg
     1861 ctacggtcgc tggtcatgtc atacaactgg aacttacgcc caagttgctg accaccgtca
     1921 cgcgtcagcg gcgtccagcc aatcgccgca cggctgcgtg tcgggccaga cgagaagaga
     1981 tccaacggta ccgagacata cacgcctttg gtgaagtccc cttcgccgta ctcctctttc
     2041 aaaacgttgg tgatcgtggc atagccaccc accaccacgc cgctgtcaaa gcgtttggcg
     2101 atctccagcg tgccgccttt atcccctgcc agatactgcc cgacgctggc tttcaccaac
     2161 acgtcctggg cgaaagatgg cgtccagtag gcggtcagat gtccggtttt cacgctgtag
     2221 tcggtgaatt tcatcatatc ttttgcacta cgccagtcgc gctgtttaac gtagttggca
     2281 tccagaccaa acgcccagtt actgtccagc ggacgataca acacttcggc ccccgcaccg
     2341 ccaaacatgg tttcgagata acctccatag acctgaccgt agaagccgtt gcccagatgc
     2401 tggaagtagt tggcttgcag gttattcaca tagacatcgt tctgcacata ctcgcgcaca
     2461 tgggtacgaa cgcgcggtaa gtgcgagtcc tgcggcggat tggtgtagtt aaacttgtcg
     2521 tagttgttgg caagatttgc aaacaggctg ccggtggtca gcagatggtc cgtcagccac
     2581 aaatctgcca ttcccatcac gcccagctga tacatgtaaa agttttccgg gccaccgacc
     2641 gactggttca gcaccggatc gatatggaaa tcaaagcgtg atttgtcgat ataccagccc
     2701 tgctcggtgg actgcggaac cactggctcg acgcgttttt gcgccagctt cgtttcgtgg
     2761 cccaacggct ctccggcgag atgatttttc aggctggcga catcggtttc cgtcgtcacc
     2821 tgcggcatgt taagacgatt ttccgtaatg cggatcgttt tgatcccatc cggcagatcg
     2881 ttcatcacga tccgattagc acggatgatc ccttcgcgcg aatcacgata tttcacctgc
     2941 tcgccggtga catacagcgt atcgcctttc gcctggatct gcggatcggc aagtccgaca
     3001 ttgtatttca acagcgttaa ctgattcgcc accaccgaat gctgcaaaat ggcatcctgc
     3061 ggctgcggtt gatattgcgg gcgggcgtta tcgttgtaag acgggcgcag atcgttaaag
     3121 ttggtgcgca gcgtgacgcc aaacataaag gtgttgccac gttcatagct aaggttaacg
     3181 tcggcccaat cggtaacgcg ataaatcgca ccgacgttaa acttactttt ttgctccagc
     3241 ttcccggcaa aatcctgctg ataattattg ccttcatact ccagtttcag gcgcagcggt
     3301 tgccagggcg tctggtattc cacgccgcca aacagtgagg caggaccgtg gaacatctgg
     3361 ctaccgccga tagatcccgc ctgtttgtag ctgttatcgc gatagcaata tttatcactg
     3421 gctgaacaga gcggattttt cacattaccg ctggtgccca gataacccca gcccaggccg
     3481 agcgtaaaat cgaacggccc ccaggctttg ctggcaacaa gatattccgc atcaaacagc
     3541 cccgtaccgc cgatatccct cgcaccaacc gccacttgcg gcagccagta actctcttcc
     3601 cacaaacgca gtttgagatc gaaggcttta tctttatacg tttgatcgcc agagaacgct
     3661 tcgacgctgc tgtactgccg ggtgcgcacg tcggtgtagc gcagcgttgt ttccagccac
     3721 gggaagagtt gcactgaagc tgagtaataa cggtactgat cgttatcgcg atagttcagg
     3781 ctcaactccc cttcccgcgc catgcgtgcg gtgggcgttt gtaataatcc tacgccaccg
     3841 aaatccgact gcgacgggcc aatgggcgca ggatatgttt cgccgtagca agccatgcta
     3901 atgcccagcg ccagtaagct aagcagatgt ctttttttca ttattggggt atccgctgcg
     3961 tcagagtctg aagaatgtcg gcgttaaggg catcaggcgt ctcactccag acggagtccg
     4021 cgaggccaac ataaataatg ctgccgggca tcggctctac gtgacgcttg ttccagtaag
     4081 ccaccggcgc tttttgcgtg cgtccgtccg ggtaaaccac ccacgcgtag ctgcgatccg
     4141 caccgctaag caggttctgc ccggagagat agctcgccac gtcgcgacct ggagtgaatg
     4201 gctgcttgcc aggacggctg ataagcccga acaacgtgac cgtggacggc ggtggtccga
     4261 cccacagcgt gtagttgccc tgcaacggtg ggttaccgcg ttcggcaacg cgcacgatat
     4321 cgggatcaag attgattttt tgcctgcccg tcactttcaa cgcctgaatt tgctggcgta
     4381 aggcgttaat ggcggcggca tcgtcggcgc tggagtctgc ggcctgttca gccagccgtg
     4441 tcagcaacgc ctgctgctga cgcaatgctg ccgccgttgc cagctcttcg ctaatcaccg
     4501 caccgggcca ccagttgttt gccagccgcg gttagcctac cagatcgatt aaatgctctg
     4561 cgcctgttag tgttttggcc tcactgctgc cattgctgaa caccttgacg gttcctgccg
     4621 cgaagaccga tgacgctccc acactcaaaa gcaacgcgac aaaagtttgt ttaatcattg
     4681 ttttgccgcc ttgatgagag tggttttcac cgggaaataa tccgcgccga gatactgttc
     4741 cgactggcga atttgccctt cgctgtcgat ccagtagcgg ttatgccagc gcgcctggtc
     4801 ggtggagact tcttcgtcca gcacgcgaac caaggtttca tcgctgccga ctttgacggt
     4861 atcggtgcca tcccatttga agactgagcg tgcggtggcg tagcgtacct gctggtactc
     4921 ggtccagccc atcgtgcgcg tccatgttgc accatcaaca atttgtgcgg gtttaatcag
     4981 cgggtcggcg gcaaggttat tcacttcaat caggttgtcg ccgccaagca aagtcttcac
     5041 cagacgacca tgttgtgtga caagagtggc ttgatcctga gtgacccatt tttgttgtcc
     5101 gtcttcagcg aaggccagca ccacaaacaa ttgcggcccg ccattaagct gcatgtactg
     5161 gctggcgtag ggcatatttt gaatatcatc gtccgtcagc tgtacgcctg gcgtgccgaa
     5221 cagactgtcc cacagtgaat tgcccagctc tttagtcgtg gctgaacagg cctgtaatag
     5281 caggcagata agaatgagtg caggtcgctt cacgactctt ctccagaggg gcagaataac
     5341 cacaccggag tgtggttatg gtaaatccct tcatacttca agccacagat gcaatgactc
     5401 aaattatttt ggggatacat actttattac tgggtactgg tggtcgtggt tgtggtggtc
     5461 ccggtattag aaccatcacc gccaccggtt gccgcgagcg cgacacccac cgccgagctt
     5521 acggtgctga cgctggtcgc ggaggaacta cccgccgaaa ccgacgttgc tgccgaccct
     5581 gccgcttcgc ccacctgtac aggtgcagcc cacgcagaag tcgccgcaag cgcagatatg
     5641 gcaaaaatgc catacagaac ttttttcata acaatttccc ttcattgaat gaatggagat
     5701 ttacccaaaa acatttcggg cgggatcgag tatacacaac taaagcatgc gaattacggc
     5761 gcggggaatt agcgatggcg ttttaggata attggaatga atatcaaata taagcttcaa
     5821 cttatattta aataacaata aaaatcataa acttaaaata aaaacaaaag taattaatct
     5881 gcgtattctc cgaaataaac aacgattaaa aatctatttt aggattaacc tcacggtatg
     5941 atttccgtta aatcacagac aaggggattt atctgataaa acaatgccga cttattgtcg
     6001 gcattgtttt taaggttatt taactcctca tactatgcat taatgtcgat attggcatca
     6061 ggctccggtt gccggatgcg gcgtaaacgc cttatccggc ctacatatcg gcatcaggca
     6121 cgcatgatga ttaaccgcgc cacgctttat agcggttaat cagaccattg gtcgagctat
     6181 cgtggctgct gatttctttg tcatctttca gctctggcag aatacggttc gccagttgtt
     6241 tacccaactc cacgccccac tggtcgaagg taaagatgtt caggatcacg ccctgagtaa
     6301 agattttgtg ctcatacagc gcaatcaacg cccccaggct gaacggagtg atttcgcgca
     6361 gcaggatgga gttggtcggg cggttacctt cgaacacttt gaacggcacc acgtagtcaa
     6421 gcgttgccgg atctttaccc tgatcgcgat attcctgctc aaccacttcg cgggatttac
     6481 caaacgccag cgcttcggtc tgggcgaaga agttagacag cagtttctgg tggtgatcag
     6541 agagcgggtt atgggtgata gccggggcga tgaaatcgca cggtaccatt ttggttccct
     6601 ggtggatcag ctggtagaac gcgtgctgac cgttagtgcc tggttcaccc cagataatcg
     6661 ggccagtctg gtaatccaca acgttaccgt tacggtcaac atacttaccg ttggactcca
     6721 tattgccctg ctggaagtac gccgcgaaac ggtgcatata ctggtcatac ggcagaatcg
     6781 cttcagtttc cgcaccaaag aaattgttgt accagatgcc aatcagcgcc agcaggacag
     6841 gcaggttttt ctcggcaggc gtggtggaga aatgcttgtc catcgcgtgt gcgccagaaa
     6901 gcagttcaac gaagttatca aagccgatgg agagaacaat cgacaggcca atcgctgacc
     6961 acaaagagta acggccgccg acccagtccc agaactcgaa catgttggca gtatcaatac
     7021 caaactcgcc aacggctttg gcattggtgg aaagcgccgc aaagtgtttt gcaacgtgtt
     7081 tttcatcacc tgccgctttc aggaaccagt cacgcgcgct atgggcgttg gtcatagttt
     7141 cctgagtggt gaaggtttta gatgctacca agaacagcgt ggtttccggg tttacttttt
     7201 tcagcacttc agcgatgtga gtcccatcga cgttagaaac aaagtgcatg ttcaggtgat
     7261 ttttgtacgg acgcagtgct tcggtcacca tgtacgggcc gaggtcagaa ccaccgatcc
     7321 cgatgttcac tacgtcagtg attgctttgc cggtataacc cttccactca ccggagataa
     7381 tcgcttccga gaaggttttc atcttctcca gcaccgcgtt gacttccggc attacgtctt
     7441 tgccatcaac cagaatcggg gtattgctac ggttacgcag cgctacgtgc agcacggcgc
     7501 ggttttcagt gcggttgatc ttctcgccag agaacatcga cttaatcgcg cccgccagat
     7561 cgcactcttt cgccagatcc tgtaatttcg ccagcgtctc ttcagtgatg cggtttttgg
     7621 agtaatccac cagcatctga tcgtcgaatg ttgcggagaa cttagaaaaa cgatcgccgt
     7681 ctttagcaaa aagatcggcg atcgtaacgt ctttcatttc atcgaagtgt ttctgtagtg
     7741 cctgccaggc agcggtctgc gttggattga tgtttttcat tagcaatact cttctgattt
     7801 tgagaattgt gactttggaa gattgtagcg ccagtcacag aaaaatgtga tggttttagt
     7861 gccgttagcg taatgttgag tgtaaaccct tagcgcagtg aagcatttat tagctgaact
     7921 actgaccgcc aggagtggat gaaaaatccg catgaccccg tcgttgacaa ccgccccgct
     7981 caccctttat ttataaatgt actacctgcg ctagcgcagg ccagaagagg cgcgttgccc
     8041 aagtaacggt gttggaggag ccagtcctgt gataacacct gagggggtgc atcgccgagg
     8101 tgattgaacg gctggccacg ttcatcatcg gctacagggg ctgaatcccc tgggttgtca
     8161 ccagaagcgt tcgcagtcgg gcgtttcgca agtggtggag cacttctggg tgaaaatagt
     8221 agcgaagtat cgctctgcgc ccacccgtct tccgctcttc ccttgtgcca aggctgaaaa
     8281 tggatcccct gacacgaggt agttatgtct gaaattgttg tctccaaatt tggcggtacc
     8341 agcgtagctg attttgacgc catgaaccgc agcgctgata ttgtgctttc tgatgccaac
     8401 gtgcgtttag tagtcctctc ggcttctgct ggtatcacta atctgctggt cgctttagcg
     8461 gaagggctgg aacctggcga gcgattcgaa aaactcgacg ctattcgcaa catccagttt
     8521 gccattctgg aacgtctgcg ttacccgaac gttatccgtg aagagattga acgtctgctg
     8581 gagaacatta ctgttctggc agaagcggcg gcgctggcaa cgtctccggc gctgacagat
     8641 gagctggtca gccacggcga gctgatgtcg accctgctgt ttgtcgaaat cctgcgcgaa
     8701 cgcgatgttc aggcacagtg gtttgatgta cgtaaagtga tgcgtaccaa cgaccgattt
     8761 ggtcgtgcag agccagatgt agccgcgctg gcggaactgg ccgcgctgca gctgctccca
     8821 cgcctcaatg acggcttagt gatcacccag ggatttatcg gtagcgaaaa taaaggtcgt
     8881 acaacgacgc ttggccgtgg aggcagcgat tatacggcag ccttgctggc ggaggcttta
     8941 cacgcatctc gtgttgatat ctggaccgac gtcccgggca tctacaccac cgatccacgc
     9001 gtagtttccg cagcaaaacg cattgatgaa atcgcgtttg ccgaagcggc agagatggca
     9061 acttttggtg caaaagtact gcatccggca acgttgctac ccgcagtacg cagcgatatc
     9121 ccggtctttg tcggctccag caaagaccca cgcgcaggtg gtacgctggt gtgcaataaa
     9181 actgaaaatc ctccgctgtt ccgcgcgctg gcgcttcgtc gcaatcagac tctgctcact
     9241 ttgcacagcc tgaatatgct gcattctcgc ggcttcctcg cggaagtttt cggcatcctc
     9301 gcgcggcata atatttcggt agacttaatc accacgtcag aagtgagcgt ggcattaacc
     9361 cttgatacca ccggttcaac ctccactggc gatacgttgc tgacgcaatc tttgctgatg
     9421 gagctttccg cactgtgccg ggtggaagta gaagaaggtc tggcgctggt cgcgttgatt
     9481 ggcaatgacc tgtcaaaagc ctgcggcgtt ggcaaagagg tattcggcgt actggaaccg
     9541 ttcaacattc gcatgatttg ttacggcgca tccagccata acctgtgctt cctggtgccc
     9601 ggcgaagatg ccgagcaggt ggtgcaaaaa ctgcatagta atttgtttga gtaaatactg
     9661 tatggcctgg aagctatatt tcgggccgta ttgattttct tgtcactatg cttatcaata
     9721 aacgagcctg tactctgtta accaacgtct ttatcgagaa taattgcctt taattttttt
     9781 atctgcatct ctaattaatt atcgaaagag ataaatagtt aagagaaggc aaaatgaata
     9841 ttatcagttc tgctcgcaaa cgaattcccg tacactgaac tctctggctc actgagggca
     9901 ccatcatggc actcccccgt attacccaaa aagagatgac tgaacgcgag cagcgtgaac
     9961 ttaaaacact gctggatcgg gcgcggattg cgcatggtcg cgtactgacc aactccgaaa
    10021 ccaacagcat taagaaagag tacatcgata aattaatggt cgagcgtgag gctgaagcga
    10081 aaaaagcccg ccaattgaag aaaaagcagg cttataaacc ggatcctgaa gcgtcattta
    10141 gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg aacaatcaac gcctgagatt
    10201 ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa ccctctcccc gaaggggcga
    10261 ggggactgtc cgagctcttt ttggacgttt tcatcagtct gaggcgttaa tcagcgtccc
    10321 ttctttttac gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg accgcctttt
    10381 tccgccgttt tttccatctt aacgactgga cgtttgatgc ccgctgtttt cggtttggcc
    10441 ttcgctttag gttttgcctc ggaagaggaa ttttcgatga gcttaaagag gtcgattaac
    10501 tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc cgcttaagct gacgttcata
    10561 atgcgcgtac gttccagctt tttcacttca tagccgaaat gctcgcacat gcgccggatc
    10621 tgacggttca gcccctgcac cagggtaatg cggaagacaa acggcgcttc ttttttgact
    10681 ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg cccccatacc acgaataaat
    10741 tcatcggtaa tcggtttatc gaccgtcacc agatactctt tctcatgatc gttacccgca
    10801 cggaggatct tattcaccag atcgccgtga ttggtgagga aaatcagccc ctgggagtct
    10861 ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta
    10921 tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg gcttgttcag ggcgataagt
    10981 accaaatctt cggcttcccg aggctcaatc aactgaccgt ttactttcac aatgtcgccg
    11041 ggtttcacct gatcgccaat ggtggctcgc ttgccattaa ggaacacatt gccttgctcg
    11101 atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta tttatttaaa
    11161 cggactgatg agtcgggcag cataaattct cctgtaaaac gcggaatata ccgtacctct
    11221 tagcggggaa aaacagggtt atgcggcaac gtcgaaatta aatgtgatct gccacgcctt
    11281 ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc ctggcaaaat aaggataatc
    11341 aacatattac gaatcgctgc acacctgtgc tgatgactac tacgcagaga aacaaatgtg
    11401 caagagaaag cgctatatct ccccatgcaa agagtgaaaa atcgctttga aaggccctca
    11461 tgatggggtt atatcagatc aagcggtgag aaagcatatt tgcacaaatg tgcagaacca
    11521 ggaggcggca atggaaaaca gtgacgatat ccgtttgatt gtgaagattg cccaactcta
    11581 ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa ctgggtattt accgcaccac
    11641 catcagccgc ttgcttaaac gaggccgcga tcagggaatt gtcaccatcg ccatcaacta
    11701 tgactacaac gaaaatctct ggctggagca gcaactgaag caaaagtttg gcctgaaaga
    11761 cgttgtggtg gtgtcgggaa atgatgagga tgaagagact caactggcga tgatggggtt
    11821 acacggcgcg caactgctgg atcgcttgct ggagcctggc gatattgtcg gtttttcctg
    11881 gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag gcggggcaat cgcggcagtt
    11941 aatctgcgta ccaattattg gcggaccgtc cggtaaactc gaaagccgct atcatgtaaa
    12001 cacattaacc tacagcgcgg cagcgaagct gaaaggggaa tcgcatctcg cggattttcc
    12061 ggctctgctg gataacccat taattcgtaa tgggatcatg cagtctcagc actttaaaac
    12121 catctctgcc tactgagata atctggatgt cgccctggtg ggaattggct caccggccat
    12181 tcgcgacggc gctaactggc atgcgtttta tggtggtgaa gagagtgacg acctgaatgc
    12241 ccgccaggtt gctgacgata tttgctcgcg cttttttgat attcacggcg caatggttga
    12301 aacgaatatg agcgaaaaaa cactctctat cgaaatgaat aaattaaagc aggcacgata
    12361 ttccattggc attgccatga gtgaagaaaa atacagcgga attgttggtg cactgcgtgg
    12421 aaaatatatt aattgtctga taacgaatag cagcacagct gaactattac tgaaataaaa
    12481 atcttcccga tgtttagcgt aataaaaatt acggcggggc cctattatct aaaatcagga
    12541 gtctgttatg caaacgtggt taaatttgca ggataaaatc attattgtca ccggcggcgc
    12601 atccggtatt ggtctggcga ttgtagagga attattagca caaggcgcaa acgtacagat
    12661 ggtagatatt cacggtggcg atggtcaata tgaaggtcat aaaggctatc agttttggcc
    12721 gaccgatatt tccagcgcca aagaggtaaa tcatacggta gcggaaatta tccagcgttt
    12781 tggtcgcatc gacggtctgg tcaataacgc cggggtcaat ttcccgcgtc tgctggtcga
    12841 tgagaaagcg cctgccgggc agtatgaact caacgaagct gcattcgaaa aaatggtcaa
    12901 tatcaaccag aaaggcgttt ttctgatgtc gcaggcggtg gcgcgacaga tggtcaaaca
    12961 acatgatggc gtgattgtga atgtttcctc agaaagtgga ctggaaggct cagaaggcca
    13021 aagctgttac gccgctacca aagccgcgct taatagcttc acgcgatcct ggtcgaaaga
    13081 attgggtaaa cacggtatcc gtgtggtcgg tatcgcgccg gggattctgg aaaaaacagg
    13141 actgcgcacg ccggaatatg aagaagcgct ggtgtggacg cgcaatatca ccgtcgagca
    13201 gctgcgtgaa ggctatacca aaaacgccat tcctattggg cgcgccggaa gattagcaga
    13261 agtcgctgat tttgtttgtt atctgctgtc tgaacgcgcc agctatatca ccggagtaac
    13321 cactaacatt gcgggcggca aaacgcacgg ctaaggaggc tttatggtca atgccatttt
    13381 ttgcgcccac ggcaaattag cttgcgccat gctggaatcg gttcagatgg tgtacggcga
    13441 cgccagggtc gaggcagttg aatttgtgcc cggcgagaac gccggagaca tcgtagcaaa
    13501 gctggaaaag ttagtaagca ttcacaatca agatgagtgg ctgattgccg tggatttaca
    13561 gtgcggcagc ccgtggaatg cggcagcgat gcttgcgatg cgcaatcctc gtctgcgggt
    13621 aatcagcggc ctttctctgc cgctggcgct cgaactggtt gataaccagg acagcatgaa
    13681 tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag caaacctgcg ttgtctggaa
    13741 acaactggca acggcagagg aggatttctg atgaacatta cgctcgcccg cattgatgac
    13801 cgcctgatcc acggtcaggt cactaccgtc tggtcgaaag tggctaacgc ccagcgcatt
    13861 attatctgca atgacgaagt ttataacgat gaagttcgcc ggacattatt gcgtcaggct
    13921 gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa aagccgtcgc tgtttatcac
    13981 aacccgcaat atcaggacga aaccgttttt tatcttttca ctcgcccaca ggatgcctta
    14041 gcgatggttc gccaaggggt aaaaatagac accctaaata ttggcggcat ggcctggcgg
    14101 cccggtaaaa aacagttaac caaagcggtt tcattagatg atgatgatat taacgcattt
    14161 catgagttaa ataatcttgg cgtcatactg gatttacgcg tcgtagcgtc agatccctca
    14221 atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa attaaataat agcgcttgtg
    14281 tacatatgct ttaacaggca gggatgacga cacttaaaag gtgaaaaatc atggaaataa
    14341 gtaccctaca aatcattgct atatttcttt tttcctgtat tgccggaatg ggcagcgtgc
    14401 tggatgaatt tcagactcat cgcccgttaa ttgcctgtac ggtgattggt ttaattctcg
    14461 gtgatttaaa aaccggaata atactcggtg gtacgctgga attgatagct ctcggctgga
    14521 tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc cagcataatc tccgccattc
    14581 tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc catcgcgttg cctgtggctg
    14641 cggcaggcca ggtgctgact gtgtttgccc gtaccatcac cgtagtgttc cagcacgcgg
    14701 cggataaagc agcggaagaa gcacggtttc gcactctcga tattctgcat gtctccgcgt
    14761 ttggcgtgca ggcgctgcgc gttgctattc cggcactgat tgtctcactg ttcgtcagcg
    14821 ccgatatggt gagcaatatg ctgagcgcga taccggaatt cgtgacgcgc ggtctgcaaa
    14881 ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt gctacgtatg atgggcgtga
    14941 aatatttgat gcctttcttt ttcctcggtt tcctcgcagg tggctacctc gatctcagtc
    15001 tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt ctacatccag ctaaatccac
    15061 agtggcgtaa agctgaacct caactccagg ccaccgcctc caccgccctt gaccaacttg
    15121 acgattaacg gagccatcat ggaacagaga aaaattacac gcagcgatct ggtgagcatg
    15181 tttctgcgct ccaacctgca acaggcgtcc tttaactttg aacgtattca cgggctgggt
    15241 ttttgctacg acatgatccc cgccatcaaa cgactttacc cattaaaaga ggatcaggtt
    15301 gcggcgctca ggcgacacct ggtgttcttc aataccacgc cagccgtatg tggcccggtc
    15361 atcggcgtca ctgccgccat ggaagaggcg cgggccaacg gcgcggaaat tgatgacggt
    15421 accattaacg gcatcaaagt cggtctgatg ggaccattgg caggagttgg cgatccactg
    15481 gtctggggaa cgctgcgccc gattaccacc gcgctcggcg catctctggc actttcgggc
    15541 aacattctcg gcccgctgct gttcttcttt attttcaatg cggtgcgtct ggcgatgaag
    15601 tggtatggcc tacagctcgg ctttcgcaaa ggggtgaata tcgtcagcga tatgggcggg
    15661 aatgtgctgc aaaaactcac cgaaggcgcg tcgattctcg gactgtttgt gatgggcgtg
    15721 ctggtcacca aatggacgtc aatcaacgta ccgttggtgg tttcacaaac gcatgccgcc
    15781 gatggctcca ccgtcaccat gaccgtgcag aacattctcg accaactttg ccctggtttg
    15841 ctggcgctcg gtctgacgct gcttatggtt cgtctgctca acaaaaaaat taacccggta
    15901 tggctgattt tcgccctgtt tggcttaggg attatcggta atgcgctggg cttcctgtcc
    15961 agattcttcg ccccggcatg actgccgggg ccatcgctca acatgaggtg gtttatgaaa
    16021 acaacagctc tgcgtcttta tggtaaacgt gatttacgcc tggaaacctt tgaccttcct
    16081 gaaatgcagg aggatgaaat cctcgcgacg gtggtcactg acagcctgtg cctctcttcc
    16141 tggaaagagg ccaatctggg tgaaaaccat aaaaaagtac ccgacgatgt ggcgaccaac
    16201 cccatcatca tcggccacga gttttgcggc gatattctgg ccgtgggtaa aaagtggcag
    16261 cacaaattcc agccgggtca gcgttatgtg attcaggcca acctgcaact ccccgaccgc
    16321 ccggactgcc ccggctactc cttcccgtgg gtgggcggcg aagccacgca tgtggttatt
    16381 cccaacgagg tcatggaaca agattgcctg ctggcatacg acggcgaaac ctattttgaa
    16441 ggctcactgg ttgaaccgct ttcctgcgtg attggcgcgt tcaacgccaa ctatcatctt
    16501 caggaaggta gttataacca cacgatgggg attcgcccgc aagggcgcat gctgatcctc
    16561 ggcggcaccg gaccaatggg actgttggcg attgattatg cgctacatgg acccgttaac
    16621 ccgtcgcagc tcgtcattac cgataccgac aacgataaat tgagttatgc gcgcaagcac
    16681 tatccatcag aaccgcaaac actgattcat tatctcaatg ccgccgatgc agcatttgat
    16741 acgctaatgg cgctgagtgg cggtcacggc ttcgatgata ttttcgtctt tgtgcctaat
    16801 gaaggactgg tgactctcgc ctcttccttg ctggcgacag atggttgcct gaatttcttc
    16861 gccggaccgc aggataaaca tttcagcgcg ccaattaatt tctacgatgt gcattatgca
    16921 tttacccact acgtgggcac gtcaggcggc aataccgacg acatgcgcgc ggcggtcaaa
    16981 ctgattgaag agaaaaaagt gcaggccgca aaagtggtaa cacatattct tgggctgaat
    17041 gccgcgggcg aaaccacgct tgaattgcct gccgtcggcg gcggcaaaaa gctggtgtat
    17101 accgggaaat acctgccgct gacgtcactc acgcagattc aggatcaagc actggcggcg
    17161 attctggcgc gtcatcaggg gatctggtcc ggtgaggcgg agcaatatct gctcactcat
    17221 gcagaggcaa tttcccatga ttaatcgcgc tacttaactc tgcatgtcgc tggctggtcg
    17281 ccgcggcaac ttcgcgacct gatgtgcggc tgattgctga agcggcccaa tgtgcacgag
    17341 aaggataaaa aaacatcagc gccttaaggg gcgctcaaat tggtggcaaa gtacacattg
    17401 tttatgccgg atgcggaata aagccttatt cggcctacat aatctcgaaa attcaatata
    17461 ttgcagttat ctggtaggcc tggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc
    17521 agtctcagct cccggtgcaa tacgtgacat cgtgagccat gtcagtaagt ggttaaattc
    17581 gcagtggtag catgccttcc gtgatttctt actaaccgga gaagtaatgg aactgctttt
    17641 attgagtaac tcgacgctgc cgggtaaagc ctggctggaa catgcactgc cgctaattgc
    17701 tgaacagttg cagggtcgcc gctcagcggt gtttatccct ttcgctggcg taacgcagac
    17761 ctgggatgat tacacagcga aaacggctgc ggttctcgct ccgctgggtg tttctgtcac
    17821 cggtattcat agcgttgtcg atcccgttgc cgcgattgaa aatgctgaga tcgtgattgt
    17881 cggcggcggg aatactttcc agttgctgaa acagtgccgc gagcgcgggc tgctggcacc
    17941 aattactgac gtggttaaac gtggtgcgct gtatattggc tggagcgcag gcgctaacct
    18001 tgcttgccca accattcgta ccaccaacga tatgccgatt gtcgatccgc aaggtttcga
    18061 tgcgctaaat ctgttcccgt tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg
    18121 ccataaaggt gaaacccgtg agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact
    18181 gacgattatt ggtctaccgg aagataactg gatcacagtg agtaaaggtc acgctacgct
    18241 gggtggcccg aacaccacct atgtgtttaa ggccggtgaa gaagcggttc cgctggaagc
    18301 tggtcaccgt ttttaagtga attgccggat gcatgatccg gcatcttgtt catagtgcct
    18361 gatgcgacgc ttgacgcgtc ttatcaggcc tacaggattc acaatattaa tactcgtccc
    18421 gtccttcatc ttcatccgac tgttccagca cactgtaagc caccgaacaa aacagcgagt
    18481 tcaggcgctg catatctccc agtaagccta aatgtagcga actggtttca atgctctgca
    18541 cgttttgctg atgcaggcga tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa
    18601 agcgatgctt actacggcgc aaacgacgag cgctggtgac atcgccagag aagaacaccg
    18661 acattgccag ctttaaatta ctgagcaatt gctcatagag cgcatccagt tccttcaacc
    18721 catcaagcga aaatgcccgc cgtgctgcca gtgatttgtc agcaatttcg ctgcccatgc
    18781 gctcgacgat atcggaggcc tgttcaaggt tgagcgacat ttcaataatc tccgcccagc
    18841 ggcgcgactc ttcctccgcc agctcttctt ttggcatccg cgccagatac agcttaatgg
    18901 cggtgtagag aacgttgata tcatctgcca gcttacgcag ctctttctcc tgccgtggct
    18961 caccgtgcat cactttattc aacccttcca tcatctgttc catggcgtcg ccaatgcgca
    19021 gggtttcgcg cgcggcgttc gccagagcaa gcgtgggcgt atccagcgcg ctgacatcca
    19081 gatgtttagg ccgtagctgg gtatccagtt ccggttcatc gcgaataatc gttttgcaaa
    19141 accgtgccat cgggtcaaca aatggcagca tgaccaggca acgtacaagg ttgtagaaga
    19201 cgtggaaata gatcaccagt tccgcttttg gcaatgacag cttccccatt gtctctgcca
    19261 gcaaatggac aaacggcagg ataatcaggc tacccaccag cttaaacagc agactaccca
    19321 gcgcgacacg gcgggctgcg gcattggcgg cactgttgtt gagcatcgcc agcaggccgg
    19381 aaccgaggtt agcaccaatc accagacaga gcgccacggg gaaggagata atgcctgcgg
    19441 cggtcagagc cgcggtcagc agtacagcag caaggctgga gtagctgata atggcgaaca
    19501 tcgcgccaat cagcgcatcc agcagaatat cgccggtcag cgaggcaaag atcacctgaa
    19561 cgccgtttgc ctgggtgatc ggcgttacgg cctgcacaat caactccagc gccagcaaaa
    19621 tcagcccaag accaataccg acgcggccca gttgcccggc gcgtgactgt ttgcgtccga
    19681 ggaaaaaaat cacgccgata aaaataagta acggtgacag ccaggataag tcgaaggtga
    19741 gaatacgcgc cattagcgcc gtcccgacat ctgcccccag cacaatgacc agagccggtg
    19801 cgagggctac cagatcctga gcgacgaacg aggtcaccag catggtggtg gcattactgc
    19861 tctgtaccag tgcggtaacg ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc
    19921 tccggctaag gacagtacgc aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa
    19981 tatgagtccc ccagaccagc agggcgacgg cagaaagcag gtgaagcagc gttaacacga
    20041 gttcaggttc tccttatcgt tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa
    20101 acacgctgac tggcatcttt actgtgcctt ttgacattcg tttttccggc taagattatt
    20161 ccagcctgat tccagtataa gtgtttcata ttaataaaga gacagggcac tcattgagcg
    20221 ccctgtttgt tgtaaggaaa agtgacagat ttgtgaatca gtccgcgtca taccccagat
    20281 tcggtgccag ccagcgctca acatctgaga cgctcatacc tttacggcgg gcatagtctt
    20341 caacctgatc gcgctgaatt tgcgccacgg cgtagtactt gctgtccggg tggctgaaat
    20401 accaacccga aaccgatgca ccgggccaca tggcgaaaga ttctgtgagt ttcatgccgg
    20461 tatgtttttc cacttccagc agctcccaga tggtggcttt ttccgtatgt tccgggcagg
    20521 ccgggtagcc cggtgccgga cggatgccct ggtagttttc gcggatcagt tcttcgttgc
    20581 tgaggttctc gttcggcgca tagccccagt agactttacg cacacgctca tggagatact
    20641 ccgcaaaggc ttcggctaaa cggtcggcaa gcgctttcac catgattttg ttgtaatcat
    20701 cgtgctgcgc ttcaaaggca tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa
    20761 atgcgccgat gtaatctgct ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt
    20821 agttagcgaa tcccgttttt tcggtctgtt gacgcagatg gtggctgaca ttgatcacat
    20881 gggtgcgcgt ttcgtcacgg tagatttcaa tgtcatcgcc cacacggttt gccgggaaca
    20941 ggcccaccac gccacgccac gcggattcag cgttttctcg gcgcttaatt tatccagcat
    21001 gtcgttggcg tctttaaaca gccgctgcgc ctcaacgccc accacttcat cttccagaat
    21061 gcgcggatac ttcccggcca gcgaccaggt cataaagaac ggtgtccagt cgatgcaatt
    21121 acgcagagtt tcgatgctgg cttcaacttc ctgcacgccg agacggtgcg ccaccggcgg
    21181 cgtgtaagcc tgccagtcaa aagcgaagtc gttatcgcgc gccgcttcca gcgtgaccgg
    21241 tggtgtgcgc ggtttcttgc gcccgtgctg aatacgtacg gtttcgtact ccttgcgggt
    21301 acgagcgaca aaatcatcgc gctgagtatc ggaaagcagc gccgccacca caccaacggt
    21361 gcgcgaggca ttctgcacat acaccgtcgg gccgctgtag ttctgctcaa ttttcaccgc
    21421 cgtgtgcgct tttgaggtgg tcgcgccacc gatcagtaac ggaatggtga agccctgacg
    21481 ctccatctct ttcgccacgt taaccatctc gtccagcgac ggcgtgataa gccccgaaag
    21541 gccaatcaaa tcagcattca cttctttagc agtacggaga attttttccg caggcaccat
    21601 cacgccgaga tcgacaattt cgtagttgtt acattgcagc accacgccaa cgatattttt
    21661 accgatatcg tggacgtcgc ccttcacggt ggcgatcacc atcttgccgt tggttttgcc
    21721 ctgctctttg ctggcttcaa taaacggttc gagatacgcc accgcctgtt tcatgacgcg
    21781 cgccgatttg accacctgtg gcaggaacat tttgccttcg ccaaacagat cgccgaccac
    21841 gttcatgccg tccatcaacg ggccttcaat cacttcaatc gggcgcgtag cctgctggcg
    21901 ggcttcttcg gtatcctgct cgataaactc ggtaatgcct ttgaccagcg agtattccag
    21961 acgtttattc acttcccacg agcgccactc cgcctgctgg gcgttatcgg tgtcgtcggt
    22021 tttgctgccg cgatatttct cggcaagctc cagtaaacgc tcagtgccat cgtcacgacg
    22081 attaagaatc acatcttcca ccgcgtcgcg cagttcagcg ggtaggtcgt cgtaaatcgc
    22141 cagttgcccc gcgttgacga tccccatatc catgccattg cgaatagcgt agtagaggaa
    22201 cactgcgtga atggcttcgc gcaccggatc gttgccacgg aatgagaaag aaacgttaga
    22261 tacgccgccg gaaatcagcg cgtgcggcag ttcgcgtttg atgtcttcac acgcgccgat
    22321 aaagtcctgc gcgtagttgt tgtgctcttc aataccggtg gcgaccgcga agatgttcgg
    22381 gtcgaagatg atatcttctg gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg
    22441 acggcaaatc tcgattttcc gtgcgcgagt atcggcctgt ccctgttcat caaaggccat
    22501 caccaccact gccgcaccgt agcgacgcaa caatttcgcg tgatggataa aggcgtcgac
    22561 gccctctttc atcgagattg agttaacaat gcctttgccc tgaatacact tcagcccttt
    22621 ttcgataacg tcccattttg aggagtcgat cataatcggc acgcgagcga tatccggttc
    22681 accggcaatc agattgagaa aacgcaccat cgccgcttcg gcatcgagca tcccttcatc
    22741 catgttgata tcgataatct gcgcgccgtt ttccacctgc tgacgcgcca catcctgcgc
    22801 ctcgctgtat ttctcttctt tgatcaggcg cttgaactta gcagagccgg tgacgttggt
    22861 gcgttcaccc acgttcacaa acaggctatc gtcgccaatg ttcagcggct ccaggccaga
    22921 caaacggcag gctacgggaa tttccggcag tttgcgcggc gctaatcctt ctactgcacg
    22981 actcatcgct gcaatatgtt gcggcgtggt cccgcagcaa ccaccgacga tattgagaaa
    23041 gcccgcttgc gcccattcac gtatctgttt tgccatcgtg tcggcgtcga gatcgtattc
    23101 accaaaggcg ttaggcagcc cggcgttcgg gtgggcggtg acgtagcatt ccgcaatccg
    23161 tgatagttcc tgcacgtact ggcgcagttc atcgggcccc agcgcacagt tcaggccaaa
    23221 ggttagcgct tcggcgtggc gcaatgagtt gtaaaatgct tcggtggtct gcccggagag
    23281 cgtgcgcccg gaggcgtcgg tgatggtgcc ggagatcata atcggcagct caacgcccag
    23341 cgcttcaaac tccgttttca ccgcaaaaac cgccgctttg gcgttaaggg tgtcgaaaac
    23401 ggtttcaatc aggatcagat ccgcaccacc ttccaccagc gctttggtgg attcgcgata
    23461 ggccgccacc agctggtcaa aagtgatgtt gcggaatgcc ggatcgttga cgtccggaga
    23521 aatagacgcc gtgcggttgg ttgggccgag aacaccggca acgtagcgcg gtttctccgg
    23581 cgtgcgcgcg gtccactcgt cagcacaggc gcgcgccagt ttcgccgccg caaagttgat
    23641 ttccgccgac agggattcca tctggtaatc cgccatcgca atggtcgtgg agttgaaggt
    23701 gttggtttcg atgatatccg cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac
    23761 ttccggtttg ctgagcacca gcaggtcgtt gttgcctttg agatcgcatg gccagtcggc
    23821 aaagcgttca ccacgaaaat cggcttcgtt cagtcgataa ctctggatca tggtgcccat
    23881 accgccgtcc agcaccagaa tacgttcatt taactgcgca cgcagttgtt ccactttgct
    23941 gctcacactt gctcccgaca cgctcaaccc agatttaata aaaattcaac aaaccatact
    24001 ggcataaacg catctgtggt aaaagcgacc accacgcaac atgagatttg ttcaacatta
    24061 actcatcgga tcagttcagc aactattgca ttagctaaca ataaaaatga aaatgattcc
    24121 cacgatacag aaaaaggaga ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa
    24181 acccgccgtt gccaccgcac cagcgactgg acaggttcag tctttaacgc gtggcctgaa
    24241 attactggag tggattgccg aatccaatgg cagtgtggca ctcacggaac tggcgcaaca
    24301 agccgggtta cccaattcca cgacccaccg cctgctaacc acaatgcaac agcagggttt
    24361 cgtgcgtcag gttggcgaac tgggacactg ggcaatcggc gcacatgcct ttatggtcgg
    24421 cagcagcttt ctccagagtc gtaatttgtt agcaattgtt caccctatcc tgcgcaatct
    24481 aatggaagag tctggcgaaa cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc
    24541 gattattatc gaccaggtac agtgtacgca tctgatgcga atgtccgcgc ctatcggcgg
    24601 taaattgccg atgcacgctt ccggtgcagg taaagccttt ttagcccaac tgagcgaaga
    24661 acaggtgacg aagctgctgc accgcaaagg gttacatgcc tatacccacg caacgctggt
    24721 gtctcctgtg catttaaaag aagatctcgc ccaaacgcgc aaacggggtt attcatttga
    24781 cgatgaggaa catgcgctgg ggctacgttg ccttgcagcg tgtattttcg atgagcaccg
    24841 tgaaccgttt gccgcaattt ctatttccgg accgatttca cgtattaccg atgaccgcgt
    24901 gaccgagttt ggcgcaatgg tgattaaagc ggcgaaggaa gtgacactgg cgtacggtgg
    24961 aatgcgctga ctttttctgg cgggcagagg caatattctg cccatcatac c
//
LOCUS       DAJTSL010000047        24813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTSL010000047 DAJTSL010000000
VERSION     DAJTSL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.047"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(82..2301)
                     /locus_tag="OO433_001732"
     CDS             complement(82..2301)
                     /locus_tag="OO433_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6050242.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSSEEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVLHPAYGNVRIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(2412..3824)
                     /gene="ftsP"
                     /locus_tag="OO433_001733"
     CDS             complement(2412..3824)
                     /gene="ftsP"
                     /locus_tag="OO433_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6050243.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDSRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3899..4636)
                     /gene="plsC"
                     /locus_tag="OO433_001734"
                     /gene_synonym="parF"
     CDS             complement(3899..4636)
                     /gene="plsC"
                     /locus_tag="OO433_001734"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6050244.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4869..7127)
                     /gene="parC"
                     /locus_tag="OO433_001735"
     CDS             complement(4869..7127)
                     /gene="parC"
                     /locus_tag="OO433_001735"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6050245.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LVSERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSLMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(7673..8155)
                     /locus_tag="OO433_001736"
     CDS             complement(7673..8155)
                     /locus_tag="OO433_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR6050246.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPKAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(8208..8600)
                     /gene="ygiW"
                     /locus_tag="OO433_001737"
     CDS             complement(8208..8600)
                     /gene="ygiW"
                     /locus_tag="OO433_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6050247.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            8752..9411
                     /gene="qseB"
                     /locus_tag="OO433_001738"
     CDS             8752..9411
                     /gene="qseB"
                     /locus_tag="OO433_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6050248.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLASEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9408..10757
                     /gene="qseC"
                     /locus_tag="OO433_001739"
     CDS             9408..10757
                     /gene="qseC"
                     /locus_tag="OO433_001739"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6050249.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPSGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            10867..11448
                     /gene="mdaB"
                     /locus_tag="OO433_001740"
     CDS             10867..11448
                     /gene="mdaB"
                     /locus_tag="OO433_001740"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6050250.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            11479..11793
                     /locus_tag="OO433_001741"
     CDS             11479..11793
                     /locus_tag="OO433_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6050251.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(11841..13733)
                     /gene="parE"
                     /locus_tag="OO433_001742"
     CDS             complement(11841..13733)
                     /gene="parE"
                     /locus_tag="OO433_001742"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6050252.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13762..14343)
                     /gene="yqiA"
                     /locus_tag="OO433_001743"
     CDS             complement(13762..14343)
                     /gene="yqiA"
                     /locus_tag="OO433_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6050253.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(14343..15170)
                     /gene="cpdA"
                     /locus_tag="OO433_001744"
     CDS             complement(14343..15170)
                     /gene="cpdA"
                     /locus_tag="OO433_001744"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6050254.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(15195..15617)
                     /locus_tag="OO433_001745"
     CDS             complement(15195..15617)
                     /locus_tag="OO433_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6050255.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(15618..16247)
                     /gene="nudF"
                     /locus_tag="OO433_001746"
     CDS             complement(15618..16247)
                     /gene="nudF"
                     /locus_tag="OO433_001746"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6050256.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHSLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            16452..17933
                     /gene="tolC"
                     /locus_tag="OO433_001747"
     CDS             16452..17933
                     /gene="tolC"
                     /locus_tag="OO433_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6050257.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSIVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            18081..18752
                     /locus_tag="OO433_001748"
     CDS             18081..18752
                     /locus_tag="OO433_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6050258.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLASCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            18758..19918
                     /locus_tag="OO433_001749"
     CDS             18758..19918
                     /locus_tag="OO433_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6050259.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLPLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(19956..20744)
                     /gene="ygiD"
                     /locus_tag="OO433_001750"
     CDS             complement(19956..20744)
                     /gene="ygiD"
                     /locus_tag="OO433_001750"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6050260.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEARGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            20887..21648
                     /gene="zupT"
                     /locus_tag="OO433_001751"
     CDS             20887..21648
                     /gene="zupT"
                     /locus_tag="OO433_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6050261.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGISPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(21706..21876)
                     /locus_tag="OO433_001752"
     CDS             complement(21706..21876)
                     /locus_tag="OO433_001752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050262.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(22136..22789)
                     /gene="ribB"
                     /locus_tag="OO433_001753"
     CDS             complement(22136..22789)
                     /gene="ribB"
                     /locus_tag="OO433_001753"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6050263.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(22893..23041)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            23163..23453
                     /gene="ubiK"
                     /locus_tag="OO433_001754"
     CDS             23163..23453
                     /gene="ubiK"
                     /locus_tag="OO433_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6050264.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            23737..24288
                     /locus_tag="OO433_001755"
     CDS             23737..24288
                     /locus_tag="OO433_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCR6050265.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILNSQSGEVTLGTPVVITFNNTNSYQELNFKVRM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            24348..>24813
                     /locus_tag="OO433_001756"
     CDS             24348..>24813
                     /locus_tag="OO433_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6050266.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNAIINNKKTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDA"
BASE COUNT         6190 a         6577 c         6166 g         5880 t
ORIGIN      
        1 tcggcatata aataaatgcc taatgagata gattctgatg taatatcccc ggtagcaatt
       61 ctgctaccgg gaacagcgaa tttaacgtcc aaccgccgct ttaggccgtt tcttcgcacc
      121 agcattcacc ggacgagatt gcgtagacga cgcttttttt gccgtagcag gcgtctgacg
      181 ctgggtcgcc atcggcgtat gtttcgtcaa cgccggacgg gtattgcggt tctggcgacg
      241 agcttcacgc atctcttcaa tggttggcgc aggcactaag caatcgcgac ggctaccaat
      301 cagatgcttt ttacccatcg cttccagcgc ctggcggatt aacggccagt ttgccggatc
      361 gtggtaacgc aacaacgctt tatgcaaacg acgctgcttg tcgcccttcg gtacgaagac
      421 gtcttcactc ttataaccaa tcttcgccag cggttttttc ccggtgtaat acatggtggt
      481 tgagttcgcc agcggcgatg ggtagaagtt ctgtacctgg tcgagacgga aacgatgctt
      541 tttcagccac agcgccagat tcaccatatc ttcatcacgc gtaccggggt gcgcggagat
      601 gaaatagggg atcagatact gctctttacc tgcctgtttt gagtaagtat cgaacagctc
      661 tttaaagcgg tcatagctgc ccatgcccgg cttcatcatc ttcgataacg gcccttcttc
      721 ggtatgttcc ggggcaatct tcagataacc gccgacgtga tgggtcgcca gttctttgat
      781 atagcgcgga tcttccacgg cgatgtcata acgcacacca gaggcaatca ggatcttttt
      841 aatgcctttc agatcacgcg cacggcgata gaggttgatc gttggttcgt ggttcgtgtc
      901 catgtgcgga caaatatccg gataaacgca cgacaaacgg cggcaggttt gctcagcgcg
      961 tggcgatttg cagcgcaaca tatacatgtt ggcagttggc ccaccgagat cggaaattac
     1021 gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc tcattaatga tcgaatcttc
     1081 ggaacggctc tgaataatgc gcccttcgtg ctcggtgata gaacagaaag agcagccgcc
     1141 aaagcagcca cgcataatgt tgaccgaaaa acggatcatt tcgtaagccg gaatacggac
     1201 attgccatag gccggatgta gcacgcgctt gtatggcagc gcaaaaacgc tgtccatctc
     1261 ttcggtagaa agcggaatag caggcgggtt gatccacaca tagcggtcgc cgtgtttttg
     1321 catcaatgcg cgggcacagc ctgggttggt ttcgtggtgc agaatacgcg aagcatgggc
     1381 gtacagcact ttatcgccct tcactttctc gaaagaaggc agcaacacgt aggttttttc
     1441 ccacggtttc gggcgcggtg gctgcacggt tacggctttg gcttcctgct ttttcggtgc
     1501 caccggtttg ttatccgcgc acggcaaatc ttcaccatac ggatgcggga ttgggtcgat
     1561 ttttccaggg gtatcaagac gggtggaatc cacgccgctc cagccaggca gggcctcttt
     1621 cacgataatc gcggtattac gcacatcgcg gatttcacta atcggctcgc ccatcgccag
     1681 acggtgcgcc acctccacca gcggacgctc accgttacca aacatcagca tgtcggcttt
     1741 cgaatccacc agcacggaac ggcgcacggt atcggaccag taatcataat gtgcggtacg
     1801 acgcagacta gcctcaatac cgccgaggat caccggtaca tctttccacg cctctttaca
     1861 acgctgggta taaaccagtg tggcgcgatc cgggcgctta cccgcgacgt tatccggcgt
     1921 gtaggcatcg tcatgacgta aacggcgatc ggcggtataa cggttgatca tcgaatccat
     1981 gttgccagca gtaacaccga aaaacagatt cggtttaccc agacgcataa agtcgtcttt
     2041 gctgctccag tccggctggg cgatgatccc gacgcgaaag ccctgcgctt ccagcatacg
     2101 accgcaaatc gccatcccga agcttgggtg atcgacatac gcgtcgccag taaccaaaat
     2161 gatgtcgcag ctatcccagc caagttgatc catctcttca ctagacatcg gcaaaaacgg
     2221 tgccggtcca aaacaggcgg cccagtactg cggccaggag aacaggtcgc gatccggttg
     2281 gatcagggag atagagctca ttttgcttcc agaaatgata aaaaaataat caaaggccgg
     2341 ggattataag ccggaacgaa agagaaatcg aaaagtattc catactcgcc ctcctcgggc
     2401 gagtatgaag attacggtac cggattgact aacagttgcc caatcgagcc acggtccgcc
     2461 atttccagcg tctgactgtt gaagtagaac gggaagtgcg cccaggaagg ctgaccgaaa
     2521 taaacaagca gctccacttg tccgtcaacc caaacggtat ctttccagcc tctgtcttcc
     2581 gggaacggca tcgcaccatt cacgttacgg atctggaaca tcacgccttc aatatggaac
     2641 gcttgcggct cgtccgcgcg taccgtccag cgttcccacg ttccttgctg cgcggtgaca
     2701 tcaatacggt tgacgtccca cagttgtcca ttaatacccg ggtcatcacc cagactgata
     2761 tcgcgactgc gaatcggcga accggccatg atttcagttg gcagcaagcg catcggaaga
     2821 ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg ttagcaccag ggtagaaacc
     2881 agaatgctgg atggctcaaa gaagccacga atacgatcaa caatgctcgc cgcttcgcca
     2941 caggtgatcg acacttcatc accgttgctc atatccacca gaatctcgcg gcgctcgccc
     3001 ggtgccaacg aaagttgctt caccgataca ggagcaggca ggaatccctg atcgccagaa
     3061 atcacatgta acgggcgact atcgctcatc tgtagttgat agcgacgaga gttcgacgcg
     3121 ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa cgtacgggct ttgtacaccg
     3181 ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta ttctggcgta
     3241 ccaaagttat ccagccgttt atcctggata atgaccggaa aatcatccac accataatgg
     3301 ttggggatag gcagcgactt gctgacttca tcttccacca gccacattcc ggcaaggccg
     3361 ttatagacct gctgagccgt gcggttggga gtattggcgt gataccacag agtagctgcg
     3421 ttctggcgaa tgggcagtac gggtgcccag tcagcgtttg gcgacatcat ccgtgccgga
     3481 ccgcccatca gcgggcctgg tacctgtagc ccggccaccg tcattgagac attttctgtc
     3541 aggcggttgc tgtaaataag cttaacatcg tcgcccttcc agacgcggat agtcggcccc
     3601 aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc ctggcgtaaa tgaccagtgc
     3661 gcacgttgta cagtcataaa cagcggttgc ccacggcgag attcaagtag cggcggaacg
     3721 ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc ggcacaaagt
     3781 gcaatccccg atgcctgaat gaactgacgc cgactgagtg acatatttgc tccatgtaaa
     3841 actggctaaa gtaataccgt gcaggaaagt gttccccgca aaaatacaga ttgccgactt
     3901 aaacttttcc ggcggcttcg cgttctgcga cttctttatc gagctcggcg attttttgtt
     3961 ccattatcga acgacaatgg gcagccagct cacgaacctg atctttgcca tactgactga
     4021 cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt gtgcagtcga ttaagattaa
     4081 tcttattcga agttgtagag acgcacacgg gaataatcgg gacgcccgcc gcaattgccg
     4141 cgtgaaatgc tccagtcttg aacggtagca ggccgcgacc acggctgcgg gttccttccg
     4201 ggaacatcca gatggaaata cggcgttttt tgaaatgatt cacgacttcc gcaatggtgc
     4261 cgtgagcttt agtgcgattg tttctgtcga tcaataagtt gccggttaac cagtacaact
     4321 gcccgaagaa ggggatccac agcaagctct ttttacctac cgtcaccgtc ggcggttgca
     4381 caatgttcga tgctgtcacc atgtcatagt tgttctggtg gttagcgata tagatagcat
     4441 tgccgtagct ttcagcatct gcaggtttgc ggcactcaac tttcaggccg aacagcggcg
     4501 caagacggcc aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc gggctgaaaa
     4561 ggcagtaaat ggagccgaat acacagacta agatgctgta aatcacggta ataataagac
     4621 gaaagatata tagcataaca acctctgaag gtctgagaga ctggcattgt acgtcacctg
     4681 aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt taatcgcaac
     4741 gcatgaataa acaacggttt caagggaatt tttatcgttt ccgggggttg aatacgctgc
     4801 cggatgacga cttaacgttt catccggcgt tccttgcaag cgggaggaaa caggccctcc
     4861 ccggcatatt actcttcgct atcaccgctg ctggcacggc gaggagagtc gatctcaaca
     4921 cgatcgatac gctgcaaacc gcgcatcaac gtaccgcggc gtccacgttc gccagtgact
     4981 ttctgtaact cttccgggcg cagtttaatt ttgcgtttcc caacatgaat ggtcagcgtg
     5041 ctttgcggcg gcagaacgta caattgcgcc agaccatctt ctccacgcgc ggcttctgcc
     5101 gatggaatgt tgataatctt gttgcctttg cccttcgaca gctgcggcag atcgcttacc
     5161 gggaacatca acatacggcc tgcctgagtg attgccagca gcatatcgga agcatcttca
     5221 atcaccaccg gcggcataac atgggcattt tccggtaagg tgatcaaagc cttacctgca
     5281 cggttacgcg ccaccagatc gttaaaggtg cagacgaaac cgtaacccgc atcggaagcc
     5341 atcagcagtt tctgatcgtc gctttccatc agcatatggt caacggtcgc cccaggcggc
     5401 aacgttaatt tgccggtgag tggttcgccc tgaccacgcg ccgacggcag cgtaatcggg
     5461 tcgatggcat agctacgacc ggtggaatca acaaacacta ccggttggtt gctcttacct
     5521 ttcaccgccg ctttgaagct atcacccgct ttataattca ggcccggcgc gtcgatatca
     5581 tggcctttag cgctgcgtac ccagcccatc agcgacagca caatggtgac aggttcagac
     5641 ggcagcatgt cgtgctcgct catcgctttc gcttcttcgc gttcctgcaa cggcgaacga
     5701 cgatcgtcac cgtaggcttg cgcgtctgcc tgcagttctt tcttcagcag gttattcatt
     5761 ttacgctcgg aaaccaaaat gccctgcaac tggtcgcgct ctttttccag ctcactctgc
     5821 tcaccgcgaa tcttcatctc ttccagtttg gcaagatgac gcagtttcag ttcgaggatc
     5881 gcttccgcct gggtttccgt aaggccaaac cgcgacatca gcgccggttt cggttcatct
     5941 tcgttacgaa tgatctcaat cacttcgtcg atgttgagaa acgccaccag caaaccttcc
     6001 aggatatgca ggcgcttgag gactttctcc agacgatagt tcagtcggcg gcgcacggta
     6061 tcgcgacgga acaccagcca ttcggagagg atttccagca ggtttttcac cgccggacga
     6121 ccatccagac cgatcatatt gaggttaatg cgatagctct tttccagatc ggtggtagcg
     6181 aagaggtggt tcatcacctg atccatatcc acgcggttgg aacgcggcac aatcaccaga
     6241 cgggtcgggt tctcgtggtc agattcatcg cgcagatcgt caaccatcgg cagctttttg
     6301 ttgcgcattt gcgcagcaat ttgctccagt acgcgcgcac ctgaaacctg atgcggcaat
     6361 gcgctgataa ccaccgcgcc atcttctttc ttccacaccg cgcgcatacg cactgaacca
     6421 cgtccgttct cgtagatttt acggatctcg gcgcgcgaag tgataatttc cgcttcagtc
     6481 ggataatccg gcccctgcac gatatccagc agctgatcga gcgtggtttt cggctggtcg
     6541 attaatgcga ttgccgcctg agctacttca cgcaggttat gcggtggaat atcggtcgcc
     6601 atgccgacgg caataccggt ggtgccgtta agcaaaatgt ttggcagacg ggcaggcagc
     6661 attttcggct cctgcaaagt gccgtcgaag tttggcaccc agtcagccgt cccctgcccc
     6721 agctcgctca atagcagttc ggaatatttc gacaaccggg attcggtgta acgcattgcc
     6781 gcgaacgatt tcggatcgtc cggcgcgccc cagttcccct gaccatcaac cagcggataa
     6841 cggtaagaga acggctgcgc catcaggacc atcgcttcat aacaggcact atcgccgtgc
     6901 ggatggtatt tacccagtac gtcaccgacg gtacgggccg attttttaaa tttggcgctg
     6961 gcattcaggc ccagttcaga catcgcatac acaatgcggc gctgaacagg tttaagacca
     7021 tcgccaataa acggcaacgc acggtccatg atcacgtaca tggagtagtt taagtaggcg
     7081 ttttccgtaa attcatgtag cgcaaggcgc tctgccatat cgctcattaa ttctgattcc
     7141 tcaacttatt cgccagcctt caaaggggca atattgccgg agatactaca ttatctgccg
     7201 cgtcgagtca caggagatca gcgtgaatag cgccgccgga cgtgctccga cggcgataaa
     7261 ggcatcaatg tgccttgata tacaactctt tgctgtagac ttgatcggca tagagggcag
     7321 cacaaggttg cgcccatgcc acaccactcg gcatgggggt ccgctccggg cgattcgaga
     7381 agttgaggtt atgcgagtcc agggaagcct aaagcatcaa agtagtcgac tgtaagtata
     7441 cggttcagcc cagtttagct gtgtctccac cagtgccccg cgcctcctgc cataagtgcg
     7501 gcttgctcat ggataccccc gagacggctt acggcgcacc ggataataaa tttcgcgtat
     7561 acatggtatt ccttaccgga gaaattataa gcccaacaac aatacgcctg tggcattgtt
     7621 cgctcaaact tatcgcgagt gatttggctc acactccggt aaagattccc catcacgcta
     7681 acggcacata aacatccgtt tccagtcgct gttctgtcat accttcggca agattggtgt
     7741 agtgaaacag aatcggtgct ttacgcattt tctcgccgct tgcaggcagc cagtggcgaa
     7801 tgatgcccca taccgtgtgt gaaatatcgt ccagctcgcc aacgtggcgg gccacggcat
     7861 aacgtccacc ggtaagttca ccattgctca caccataacg attatcggga atcggttcgc
     7921 taacgctgcc gcagatatca aagcgaaacg ctttcggtgc ggtggtggca gggtcgtccc
     7981 aggcgacgcc aaaagtctgg ctttggttaa caggagaaag ccccgtctct ttacgccaca
     8041 tgataaattt cgccacactg tagttgagca actcagggct acagcggtgc ggcaacatcg
     8101 ccacagggat tgatgggaaa tcgataatgt ttacatccag tgtcaggttt gtcattttgc
     8161 ctccctgcga aattattccc gggaacgcgt cccgggagcg gtaacaatta cggatttact
     8221 ttgcggatct gtttgacgtc aatttcaaca gaattccagt ctttatcgac ttccccctga
     8281 atctcaaccg tatctttcgg cgtcaccgtc acaccgttcc agcgtttgtg gtcgatatca
     8341 acattgatag taccgctggc atctttaaac acgtagagat cgtcagagat gcgttcaacg
     8401 atattgccgc gcagggttac ccaggtgtcg tcacgcaggg attttgcgct ttctacagtc
     8461 gttacgctgc cgttcggccc ctggaatcct ccggcctgac tttgcgttgc cgatgggcca
     8521 gaaaaaccgc cctgctctgc tgccatcacc ggtgcactgc acagggccat tactgcgatt
     8581 actgctgcga attttttcat gtttattact ccctttaatg tctgtttccg agcatttaac
     8641 aagatagtcc ttaacaactt cttaagggaa aaaaataaaa tttaatgctg tacagagcgc
     8701 gttacaacac ggtttactgg cagcaaatac ggttatcgca gggatgaaaa aatgcgaatt
     8761 ttactgatag aagatgacat gctgattggc gacggcatca aaactggcct tagtaaaatg
     8821 ggttttagtg tcgactggtt tacacaaggt cgtcagggaa aagaggcgct ttatagcgca
     8881 ccttatgatg cggtgatcct ggatttaacc ttaccaggca tggatggtcg cgatattttg
     8941 cgcgaatggc gagagaaagg tcagcgtgag ccggtactga tcctgaccgc gcgtgatgcg
     9001 ttagcggaac gtgtagaagg gctgcgtctg ggagctgacg attatctgtg taaacctttt
     9061 gcgttgatag aagtcgccgc caggctggag gctctgatgc gccgaaccaa cggccaggcc
     9121 agcaacgagc tgcgccacgg taacgtcatg ctcgaccccg gcaaacgtat cgccacgctg
     9181 gctagcgaac ccttaacact gaaaccaaaa gaatttgccc tgctggaatt actgatgcgt
     9241 aacgctggtc gggtactgcc gcgcaaactg attgaagaga aactgtatac ctgggacgaa
     9301 gaggtcacca gtaatgccgt tgaagtgcat gtgcatcatc tgcgacgcaa actcggtagt
     9361 gattttattc gtaccgtgca tggtattggc tacacattag gtgagaaatg aaatttaccc
     9421 aacgtcttag tctgcgcgtc aggctgacgc taatcttttt aattctggcc tcggtgacct
     9481 ggctgctttc cagctttgtc gcctggaaac aaacaacgga taacgtcgat gaattgttcg
     9541 acacccaact gatgctgttt gccaagcggt taagtacgct cgatctcaac gaaatcaacg
     9601 cggcggatcg catggcacag acgccaaata aattaaaaca cggtcatgtt gatgacgatg
     9661 cgctgacctt tgccatcttt acccacgacg gcagaatggt ccttaatgat ggcgataacg
     9721 gagaagatat tccctatagc tatcaacggg aaggttttgc tgacgggcaa ctggtcggtg
     9781 aagacgatcc ttggcgtttt gtctggatga cctcacctga tggcaaatat cgcatcgttg
     9841 ttggccagga atgggaatac cgtgaagaca tggcgctggc gattgttgcc gggcaattga
     9901 tcccgtggct ggtcgcactg ccgattatgt taatcatcat gatggtacta ctgggtcgtg
     9961 aactcgcgcc gctgaacaaa ctggcgctgg cactacgtat gcgtgaccct gactcggaaa
    10021 aaccactaaa cgcgactggc gtacccagcg aagtgcgacc actggttgag tcgctaaatc
    10081 aactgttcgc ccgcacacat gcgatgatgg ttcgagaacg acgctttacc tccgatgcag
    10141 ctcacgaact acgtagcccg ttaacagcgc tgaaagtgca aaccgaagtt gcgcagctct
    10201 ctgacgatga tccgcaggca cggaaaaaag cactgctcca attacattcc gggatcgatc
    10261 gcgctactcg tctggttgat caactgctca cgctatcgcg gctggactca ctggataacc
    10321 ttcaggacgt cgcggagatc ccgcttgaag atctcctgca atcgtcggtg atggatattt
    10381 accacacggc gcagcaggcg aaaattgacg tgcgactgac actcaatgtc cagggcatca
    10441 aacgcaccgg acaaccgcta ttgctaagtt tgttggtgcg aaatttgctg gataacgccg
    10501 tgcgttacag tccacagggc agcgtggtgg acgtcacgct gaatgccgat aatttcatcg
    10561 tgagggataa cggccccggt gtgacaccag aggcactggc gcgaattggc gaacgcttct
    10621 atcgcccctc cggacaaacc gctaccggca gtggacttgg gctatcgatt gtccagcgaa
    10681 tcgccaagct gcatggcatg aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg
    10741 cgaaagtaag ctggtaaggg taagactttt gctaaattcg caaaagactt tgcacatttt
    10801 gctaatttca ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc accttgaggt
    10861 taaaaaatga gcaacattct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt
    10921 caactgaatg acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat
    10981 gttcgcatcg ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc
    11041 tgggctgatg tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg
    11101 aaaaaataca ttgatgatgt attcaccgaa ggtcacggga cgctgtatgc cagcgatggc
    11161 cgtacccgca aagatccgtc aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa
    11221 tatatgcttt ctctgacctg gaacgcacca atggaagcct tcaccgaaaa agatcaattc
    11281 ttccacggcg ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctt
    11341 ggcatggaac cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc
    11401 cgctatactg aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg
    11461 ctttagaagg agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa
    11521 catcaccgtc aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa
    11581 gaaggttgcc acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct
    11641 atggcaccgg attctatcgt gatgattgag cagtgggaaa gcatcgcgca ccttgaagcg
    11701 catctgcaaa ccccgcacat gaaggcgtac agcgaagccg taaaaggtga cgtgctggag
    11761 atgaatattc gtattctgca gccagggatt taatcctgcc ttgtttgccc ggccatcctg
    11821 accgggcaat gttctttcct ttaaacctca atctccgcca tgtcgccttt ctcttgcaac
    11881 cagttgcggc gatcttccga gcgtttcttc gccagtagca tgtccatcat cgcgtcagta
    11941 cgctgatcgt cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc
    12001 gtggtttcgc gcaattgcat cgggttcatt tcccccagac ctttaaaacg ctggacgttc
    12061 ggcttgcctt tcttgcgttt taattgctca agtacgccct ctttctcttc ttccgtcagc
    12121 gcgtaataaa cctctttccc gagatcaata cggtagagcg gtggcagtgc gacgtaaacg
    12181 tgaccgtgtt tcaccaacgc gcggaagtgt tttacaaaca aagcgcagag cagcgtggca
    12241 atgtgcagac catcggagtc agcatccgcc aggatacaga tcttgccgta acgaagctgg
    12301 ctcaaatcgt cgctgtcagg atcgataccg atcgctaccg aaatatcgtg cacttcctgc
    12361 gaagccagca cttcgtcgga agagacttcc caggtgttaa ggatcttacc tttcagtggc
    12421 atgatcgcct gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct
    12481 tccacaagaa acagttcggt gcggttaagg tcctgcgcgg tacagtcagc cagtttgcca
    12541 ggcagcgccg gaccgctggt cagcttttta cgcaccactt ttttagccgc acgcatacga
    12601 cgctgggcgc tggaaatcgc catctctgcc agcaactccg ccgcctgaac gttctggttc
    12661 agccacagga taaaggcatc tttcaccacg ccagaaacga atgccgcgca ctgacgcgaa
    12721 gagagacgct ctttggtctg cccggcaaac tgcggatcct gcatttttac tgacagcaca
    12781 taggcgcagc gatcccagat atcttccgcc gacagcttta caccgcgcgg cagaatgttg
    12841 cggtattcac agaactcacg catcgcgtcc agcaggccct gacgcagacc attaacatgg
    12901 gtaccgccct gcatcgttgg gatcaggttg acgtagcttt cggtcagcag ttcaccgcct
    12961 tccggcagcc acagtagcgc ccagtccacc gcttcagtat cgccagcgaa attaccgata
    13021 aacggttttt ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta atcattcaga
    13081 ccgtcctgat agcaccagcg ctgttcggtg ttgttgatct catctttaaa ggtaatctca
    13141 acgccggggc acagtactgc tttggctttc agcacatgcg tcaggcgtga aacagaaaaa
    13201 cgcgggctat cgaagaaggt ttcatccggc cagaaatgga cgctggttcc ggtattgcgt
    13261 ttaccgcaag tgccgacaac ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg
    13321 ttataaatct gaccatcgcg gcgcacgtta acttctacgc gcttcgacag ggcgttaacc
    13381 accgaaatcc ccacgccatg caggccgcca gagaactggt aatttttgtt agagaattta
    13441 ccgcctgcat gcagacggca aagaatcagt tcaaccgccg gtacaccctc ttccgggtga
    13501 atatccaccg gcatcccgcg cccatcgtca ataacttcta acgactggtc agcatgtaaa
    13561 ataacgtcca cgcgttttgc gtgacccgcc agtgcttcat ccacactgtt atcaatgact
    13621 tcttgcccca aatggttagg gcgagtggta tcggtataca tccccggacg gcggcgaacc
    13681 ggctcaagcc cggtgagtac ctcaatggca tcagcgttat aagtttgcgt catggtttaa
    13741 gttagtaatt cgagttgatc gtcagagatg gtgcagacca agaaaatcga cgatcgggtt
    13801 gaaataatct tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag tctggcggca
    13861 ggaagcgtag tacgccaccg cctggcggta atccagcact tcatctcccg tctgttgcag
    13921 cagccagatc aaatccggcg cttccagcgg gtcaatctgc atgactttaa gatcgtaaat
    13981 atggcgtgac tctagcacat attgctgccc ggtgtagggg ttctcgttct gaccgagata
    14041 gtccgtcagc agttcaaacg ggcgcaccgc cgggtttacc accactgcgg gcagcataaa
    14101 acattgtgac aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa tacccagcga
    14161 atcgccgcca tgttccagga caatggattc cagcagctct gccgcgtcgg aaggatacgg
    14221 cggcaactgc ggaatgatca tctcaacgtc agggtgatgt tccgccagcc agttttttaa
    14281 caagctcgct tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga
    14341 catcagtagc cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc
    14401 tcggtggtca gcgtgccatc agcatgtaac tcgagagtac gccagccggg cgcgatggta
    14461 tccagcgtaa agttggaaca gtgcggctta aactgcacac aggtcgacgg cgttgccagc
    14521 aggcggcgac cgttccagtc gagatccagc tcctgatgaa tatgaccgca cagcaagtat
    14581 ttgacgtgcg gaaacttcgc cagcacggta tccagttcgc ccgcgttacg cagactgtgt
    14641 tgatcgagcc aactacaacc cgcaggtagc ggatgatgat gcagcagcag caacgtatgg
    14701 cgttctggcg catcggccag tttacgttcc agccactcaa gctgaaactc gctcagctca
    14761 ccgtgcggca cgccaaacac ctggctatcc agcaacagga tttgccattg ctcaccaata
    14821 aacacgcgct tcgccgggga gatacccgca tcctgtaacg cgctgtacat cgcgggctgg
    14881 aaatcgtggt tgcccggcag ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg
    14941 aaatgctgat aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg
    15001 tcgaattcgt gctgatgtgg acgaatcgcc tccagcaccg cctggtagct ttcccaggtg
    15061 tttaccccta acagggcttc gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa
    15121 atcctgactc tggcctcacc agccagagga agggttaaca ggctttccaa atggtgtcct
    15181 taggtttcac gacgctagta aaccggaatc gccatcgctc catgtgctaa acagtatcgc
    15241 aaccagtccg ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg caacttttta
    15301 ttaggataat cataccgcgc tttgaagcga aaaatctgct ggcttgaaca cacttcagcc
    15361 accatcgcgt catgatacag acgcaccgtc attgacggaa ggctccagta actgatcgcg
    15421 ggcgcagtct gttctattgt caccagggta gtgtatcggg tcgattccac aatcgtcagc
    15481 cgatattgtg cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc attgcgcggt
    15541 aacaaacggc gcaattgtga aaagttcatc tcgcacaggc gcatcatttc aggaaagtca
    15601 ggtgtgtaac gcttcattta tgcccactca ttttttaacg cttggtggtg cagctgcagc
    15661 cattgcaaag cgatgaccga cgctgcgttg tcgattttcc cctcttctac ccactggtat
    15721 gcctgttccc ggcttaccac atgaacgcga atatcttcgt tttcatcagc cagaccatga
    15781 ataccgcttg cggtcgtggc gtccacttcg cccaccataa ttgacgaacg ctcactggtg
    15841 ccccccgggc ttgccaggaa acttaacacc ggtttggtcc gtttgactat cagccccgcc
    15901 tcttcaatcg cttcgcgacg ggcaacatct tccacacttt caccctcttc aatcatcccg
    15961 gcaaccatct ccagtagcca gggggtttcg ctagtgtcgt acgcggcaat tcgaatctgc
    16021 tcaatcagca caacttcatc acgcactggg tcaaagggta gcaagactgc ggcgtgaccg
    16081 cgctcaaaaa tttcccgccg cacctcatga ctcatttgcc cgttgaatag actatgacga
    16141 aatctataaa gatctaatga aaaaaagccg cgataaagtg tttctcgtgc aataatttct
    16201 acatcgtttt tgccaaatgt aacgggcagg ttgtctggct taagcattgt taatgtcctg
    16261 gcactaatag tgaattaaat gtgaatttca gcgacgtttg actgccgttt gagcagtcat
    16321 gtgttaaatt gaggcacatt aacgccctat ggcacgtaac gccaaccttt tgcggtagcg
    16381 gcttctgcta gaatccgcaa taattttaca gtttgatcgc gctaaatact gcttcaccac
    16441 aaggaatgca aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt tctgggttca
    16501 gttcgttgag ccaggccgag aacctgatgc aagtttatca gcaagcacgc cttagtaacc
    16561 cggaattgcg taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc
    16621 gcagtccatt actgccacag ctaggtttag gtgcagatta cacctatagc aacggctacc
    16681 gcgacgcgaa cggcatcaac tcgaacgcga ccagtgcgtc cctgcagtta actcaatcca
    16741 tttttgatat gtcgaaatgg cgtgcgttaa cgctgcagga aaaagcagca gggattcagg
    16801 acgtcacata tcagaccgat cagcaaacct tgatcctcaa caccgcgacc gcttatttca
    16861 acgtgttgaa tgctattgac gttctttcct atacacaggc acaaaaagaa gcgatctacc
    16921 gtcaattaga tcaaaccacc caacgtttta acgtgggcct ggtagcgatc accgacgtgc
    16981 agaacgcccg cgcgcagtac gataccgtgc tggcgaacga agtgaccgca cgtaataacc
    17041 ttgataacgc ggtagagcag ctgcgccaga tcaccggtaa ctactatccg gaactggcgg
    17101 cgctgaatgt cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag
    17161 aagccgaaaa acgcaacctg tcgctgttac aggcacgctt gagccaggac ctggcgcgcg
    17221 agcaaattcg ccaggcgcag gatggtcact taccgacgct ggatttaacg gcttctaccg
    17281 ggatttctga cacctcttat agcggttcga aaactcgtgg tgccgctggt acccagtatg
    17341 acgacagcaa tatgggccag aacaaagtgg gcctgagctt ctcgctgccg atttatcagg
    17401 gcggaatggt taactcgcag gtgaaacagg cccagtacaa ctttgttggt gccagcgagc
    17461 aactggaaag cgcgcatcgt agcatcgtgc aaaccgtacg ttcctccttc aacaacatta
    17521 atgcatctat cagtagcatt aacgcctaca aacaagccgt agtttccgct caaagctcat
    17581 tagacgcgat ggaagcgggc tactcggtcg gtacgcgtac cattgttgat gtgttggatg
    17641 caaccaccac gctgtacaac gctaagcaag agctggcaaa tgcgcgttat aactacctga
    17701 ttaatcagct gaatattaag tcagccctgg gtacgttgaa cgagcaggat ctgctggcac
    17761 tgaacaatgc gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc
    17821 cggaacagaa tgctattgct gatggttatg cgcctgatag cccggcaccc gtcgttcagc
    17881 aaacatccgc acgcactacc accagtaacg gtcataaccc tttccgtaac tgatgacgac
    17941 gacggggctt cggccccgtc tgaacgtaag acaacgtaaa gatacgggtt atctgccgca
    18001 ttcttccccc ttctcgcttc aatttcgacc agccatcctc tattctgatg ggtatttacc
    18061 actggtcccg gaagacaaaa atgaaacgga caaaatccat acgccacgca tcgttccgca
    18121 aaaactggag cgcacgccat ctgacaccag tcgctctcgc ggttgccact gtttttatgc
    18181 tggctagctg tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct gacgactgtt
    18241 cagctgcaaa cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag
    18301 aagccgaacg tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg
    18361 aaggtcagtg ccagcaggca ccagcccagg ctggcatggc accagaaaac caggcgcagg
    18421 cccagcaatc cagcgggagt ttctggatgc cgctgatggc cggttacatg atggggcgtc
    18481 tgatgggcgg cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa aacccagcca
    18541 gcccggctta cggtaaatat accgacgcga cgggtaaaaa ctatggcgca gcccagccag
    18601 gccgcaccat gaccgtaccg aagacagcca tggcaccaaa accggcgacc actactaccg
    18661 ttacccgtgg cggttttggt gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa
    18721 ccggcacctc ttctcgttca atgggtggct gataccgatg gaaagagtca gtattaccga
    18781 gcgcccggac tggcgtgaaa aagcccacga atacggtttc aattttcaca ccatgtacgg
    18841 cgagccgtac tggtgtgaag atgcttacta caagttgccc ctcgcccagg ttgaaaagct
    18901 ggaagaagtc accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc
    18961 cagcgatgag ctgatgacca aattccgcat tccaaaacac acctggagtt ttgtgcgtca
    19021 gtcatggctg actcaccagc catcgcttta ttcgcgtctt gatctggcat gggatggcac
    19081 tggcgaacct aaactgctgg aaaataacgc cgatacacca acgtcactat acgaggcggc
    19141 gttctttcag tggatctggc tggaagatca gcttaacgcc ggtaacttgc cggagggcag
    19201 cgaccagttt aacagtctgc aagaaaagct aatcgatcgc tttgtcgagt tgcgtgaaca
    19261 gtatggcttc cagttgctgc atctcacctg ctgtcgcgac acggtggaag atcgcggaac
    19321 cattcagtat ttgcaggact gcgcgacgga agctgaaatc gctaccgagt tcctctacat
    19381 tgacgatatc gggttaggtg aaaaaggtca gttcacggat ttacaggatc aggtaatttc
    19441 caacctgttc aagctgtatc catgggaatt tatgttgcgt gagatgttct ccaccaagct
    19501 ggaggatgca ggcgtacgct ggctggaacc ggcgtggaag agcattatct ccaacaaagc
    19561 gcttctaccg ctactgtggg agatgttccc gaatcacccg aacctgctgc ccgcttattt
    19621 tgcggaagat gatcatccgc aaatggaaaa atatgtggtt aaaccgatct tctcccgtga
    19681 aggcgcaaac gtgtcgatca ttgagaacgg caaaaccatt gaagcagcgg aaggtccgta
    19741 tggcgaagaa gggatgattg ttcagcaatt ccatccgtta ccgaaattcg gcgacagcta
    19801 tatgctgatt ggtagctggc tggtgaacga tcaacccgcc ggaattggca ttcgtgaaga
    19861 ccgtgcattg atcacccagg atatgtctcg gttttatcca catatttttg ttgagtaatc
    19921 aatgatgccg gatggcattc gccatccggt aattgttaac ctatctgcac cgacagcata
    19981 ctcaggctgc ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac
    20041 gcacctaaga catacaacaa cggcagatag tgctccggcg ttgggttcga taacgcgcca
    20101 ccttcatggt cgaggtaatt caccagagga tgctgctcta ccgccccttg ccacgtcaga
    20161 ttcgctttca catactcatt aaacgacatc gcccaaggat atggtgaact atcaccgtgc
    20221 cacttcactg tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tattccttca
    20281 tctcgcagcg ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg
    20341 tcgatactca actgcaccat cgggatatcg gcgtcaggat acatcttaat cagcacgccc
    20401 caggaaccgt ggtcaaagcc cctggcttct ttatccagcg tcaccgggac cggcgctaac
    20461 agctcaacca gatgctgtgc cagcacaggc gaacccggag caggataatg cgtatcgtac
    20521 agcgcctgcg ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact
    20581 cctgttccac gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat
    20641 gtcatcccca acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga
    20701 ctaccgtgac ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact
    20761 aagggtgtca ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa
    20821 caatgatgat gatcatcagt tattttgacg atctgcctga aggtgaagat ttataaggag
    20881 ttgtcgatgt cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc
    20941 gcgtttctcg gcgttctcgg acaaaaaccc tcgaaccgct tactggcgtt ttcgctaggt
    21001 tttgcggcgg ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca
    21061 gctgaaggaa tatcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctac
    21121 tttggcctgg accgcatgtt gccgcatgcg catccgcagg atttaatgca aaaatcggtg
    21181 cagccgttgc caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg
    21241 cataacttcc cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga
    21301 tttggcatcg cactggccgt cgcgttgcac aatatccctg aaggtctggc agtggcaggc
    21361 ccggtttatg cggcaacggg gtctaaacgc accgcgattc tgtgggcggg gatttctgga
    21421 ctagcagaaa ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg
    21481 gtggtcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga attaatgccg
    21541 ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg tggaatgtca
    21601 gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa acaacatcag
    21661 aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt ctaccttttc
    21721 ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca gtgcgtctct
    21781 gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca ccacaaccag
    21841 agcaatcaat acaatccagt accaggcgat aaacatcctt tacccttaat atttaacaat
    21901 actaaacatt tagcgcataa atttcacata tcctttttcg gatatattca tatggtcgca
    21961 taataacgga acaattatat gattttgatc ataatgaaaa agaatgcctg atattaatga
    22021 aattttctca tagaaagaga agcggaatta acatcttgca tagcagggca aaataaaaac
    22081 cgaagccagc tacaacttcg gtttcttgat taaggcagta attaagcagc ggttttcagc
    22141 tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca
    22201 tattgtgttt attggcaaac tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat
    22261 tagtcagctc acacagcaca ccagccggtt taaagcctgc cagcgtcatc aaatcaatag
    22321 tcgcttcagt atgaccgcca cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa
    22381 cgtggcccgg gcgattcaga tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg
    22441 tcgtaatacg atcagcggca gaaacaccgg tagtcacacc ttcagccgct tcaatggtca
    22501 cggtaaaacc ggtgccatag gcgctggtgt tattttctac catcattggc agatcaagtt
    22561 gtttacggcg atcttcagtg atgcacaggc aaacaatacc gctaccgtgg cgaatggtca
    22621 gcgccatctg ctcaacggtc atggtttctg ccgggaagat catatcgcct tcgttttcac
    22681 ggtcttcatc atcaagcacc attacaccgc gtccttcacg cagcgcagcc agtgcatttt
    22741 caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaaa
    22801 ctcactaaaa ttatggttac cagaatcagg gcagtcttag gagtggcggc atatagccaa
    22861 aataacgtga gcgggtccat gcccgacaga atcgttactc tctcccatcc ggactctaac
    22921 cgtcggcccc ggaattacac cggatctgct gtcctttgag ttcgcaccca aagcgctcgc
    22981 gggctttcaa ctgagttgat ttaccgccgg tggggaattt cgccccgccc tgagaataag
    23041 cggattcact ataacgctaa tgattagcgg cagcaacgca tagcttcaca taattctggt
    23101 ttatgactta cccttatcgc actacaatgg tactcaacac ctatcagtac agggaaacta
    23161 caatgattga cccgaaaaaa attgagcaaa tcgctcgcca agttcacgaa tcaatgccta
    23221 aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc
    23281 agctgacgcg cctcgacctt gtaagccgtg aagagttcga cgtccaaacg caagtcctgt
    23341 tacggactcg tgagaaactg gcgctgcttg aacaaagaat gagcgaactg gaaaatcgca
    23401 gtactgagat taaaaagcag ccagatccag agactctccc cccaacgctg taaacatttg
    23461 tctttaatgc actgtaaatt atgaattaat cttctaattt acagtgcttt ctattacctt
    23521 atcgcttaat tccattcaga atatcaccac aaaaatgaat gagtgcaatt gaaaatataa
    23581 ttgttatgta tttttttaag ttttacttaa tccatattta gataaactaa tttcataaaa
    23641 cccgaaaagt aaaatataat atttgaatga ttagcaatcc ttaaataaca gttactaatc
    23701 attcattttc tacttatatt tatataaagg aatattatgt ctgcttttaa aaagtcactt
    23761 cttgttgctg gcgtagcaat gattctttcc aataacgtat ttgctgacga aggtcacggc
    23821 attgttaaat ttaaaggtga agttatctct gctccatgtt caattaaacc aggtgacgaa
    23881 gacttaactg taaatctggg tgaagtagca gataccgttc tgaaaagcga ccagaaatct
    23941 ttggctgagc ctttcactat tcacctgcaa gactgcatgt tgagccaggg tggaaccact
    24001 tattcaaaag cgaaggttac tttcaccaca gcgaatacta tgacgggtca gactgacctg
    24061 ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag gcgttggcgt acgtattctg
    24121 aatagccaga gcggcgaagt gactttaggt actccggtag taattacatt caacaatact
    24181 aacagctatc aggaactgaa cttcaaagtt cgtatggaat ccccgggcaa agatgctacc
    24241 ccgggcaacg tttacgctca ggctgactat aagattgctt acgagtaatc acattaggcc
    24301 gggttcgccc ggccacatca ttaacggatt aatgataagt ggaacagatg tataaaaaat
    24361 taaaattaac cacaataagc gaattgatta aaaatattta ttgttcatta tccgttctca
    24421 tcattggttg tgcgtcagct tatgccgttg aattcaacaa agatttaatc gaagccgaag
    24481 atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg ccaattaccc gtaggtaaat
    24541 actcattaaa cgctattatt aataataaaa agacgccaat ccatctggac ctgcaatggg
    24601 tattaattga taaccaaact gcagtttgtc tgactccaga gcaattaaca ttattaggct
    24661 ttactgatga gattattgaa gaggctcagc aaaacctgat cgatggttgt taccctatcg
    24721 aaaaagaaaa acaaattaca acttatctcg ataaagggaa aatgcaatta tccatatctg
    24781 cacctcaggc gtggttaaaa tacaaagatg caa
//
LOCUS       DAJTSL010000048        24665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTSL010000048 DAJTSL010000000
VERSION     DAJTSL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.048"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..421
                     /gene="speG"
                     /locus_tag="OO433_001757"
     CDS             <1..421
                     /gene="speG"
                     /locus_tag="OO433_001757"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCR6050267.1"
                     /translation="TTSHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIIS
                     PEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHE
                     FFINGQYRNAIRMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(424..1134)
                     /locus_tag="OO433_001758"
     CDS             complement(424..1134)
                     /locus_tag="OO433_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6050268.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            1242..1547
                     /locus_tag="OO433_001759"
     CDS             1242..1547
                     /locus_tag="OO433_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6050269.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSANVSAPAQSTQDGAPAEPQ"
     gene            1746..2312
                     /locus_tag="OO433_001760"
                     /pseudo
     CDS             1746..2312
                     /locus_tag="OO433_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            2294..2422
                     /locus_tag="OO433_001761"
                     /pseudo
     CDS             2294..2422
                     /locus_tag="OO433_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
     gene            2465..3079
                     /gene="dmsD"
                     /locus_tag="OO433_001762"
     CDS             2465..3079
                     /gene="dmsD"
                     /locus_tag="OO433_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6050270.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQYEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELSRLTLAQWQSQLLIPVAVKPLFR"
     gene            3238..4530
                     /gene="clcB"
                     /locus_tag="OO433_001763"
     CDS             3238..4530
                     /gene="clcB"
                     /locus_tag="OO433_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6050271.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTEMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4483..5178)
                     /gene="bioD"
                     /locus_tag="OO433_001764"
     CDS             complement(4483..5178)
                     /gene="bioD"
                     /locus_tag="OO433_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6050272.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(5303..6523)
                     /gene="mlc"
                     /locus_tag="OO433_001765"
                     /gene_synonym="dgsA"
     CDS             complement(5303..6523)
                     /gene="mlc"
                     /locus_tag="OO433_001765"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6050273.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNLGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPIISDSIRQQALPTYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLHG"
     gene            complement(6658..7551)
                     /locus_tag="OO433_001766"
     CDS             complement(6658..7551)
                     /locus_tag="OO433_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6050274.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRAQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            7658..8910
                     /locus_tag="OO433_001767"
                     /pseudo
     CDS             7658..8910
                     /locus_tag="OO433_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9307..9621
                     /locus_tag="OO433_001768"
     CDS             9307..9621
                     /locus_tag="OO433_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6050275.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTTKAAPAKT
                     THHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQP
                     AA"
     gene            9714..9797
                     /locus_tag="OO433_001769"
     CDS             9714..9797
                     /locus_tag="OO433_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050276.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            9897..10718
                     /locus_tag="OO433_001770"
     CDS             9897..10718
                     /locus_tag="OO433_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6050277.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTAQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVVLRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(10757..11086)
                     /gene="mdtI"
                     /locus_tag="OO433_001771"
     CDS             complement(10757..11086)
                     /gene="mdtI"
                     /locus_tag="OO433_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6050278.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(11073..11438)
                     /gene="mdtJ"
                     /locus_tag="OO433_001772"
     CDS             complement(11073..11438)
                     /gene="mdtJ"
                     /locus_tag="OO433_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6050279.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            11850..12884
                     /gene="tqsA"
                     /locus_tag="OO433_001773"
     CDS             11850..12884
                     /gene="tqsA"
                     /locus_tag="OO433_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6050280.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(12909..14297)
                     /gene="pntB"
                     /locus_tag="OO433_001774"
     CDS             complement(12909..14297)
                     /gene="pntB"
                     /locus_tag="OO433_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6050281.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQDVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(14308..15840)
                     /gene="pntA"
                     /locus_tag="OO433_001775"
     CDS             complement(14308..15840)
                     /gene="pntA"
                     /locus_tag="OO433_001775"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6050282.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     TGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRRVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            16364..17308
                     /locus_tag="OO433_001776"
     CDS             16364..17308
                     /locus_tag="OO433_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6050283.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            17494..18876
                     /locus_tag="OO433_001777"
     CDS             17494..18876
                     /locus_tag="OO433_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6050284.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWFVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLVLGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAASLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            18913..19635
                     /gene="folM"
                     /locus_tag="OO433_001778"
     CDS             18913..19635
                     /gene="folM"
                     /locus_tag="OO433_001778"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6050285.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKLGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(19632..19944)
                     /locus_tag="OO433_001779"
                     /pseudo
     CDS             complement(19632..19944)
                     /locus_tag="OO433_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
     gene            20070..20789
                     /gene="rstA"
                     /locus_tag="OO433_001780"
     CDS             20070..20789
                     /gene="rstA"
                     /locus_tag="OO433_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6050286.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGITICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     MPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRNVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            20793..22094
                     /gene="rstB"
                     /locus_tag="OO433_001781"
     CDS             20793..22094
                     /gene="rstB"
                     /locus_tag="OO433_001781"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6050287.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            22170..23099
                     /gene="tus"
                     /locus_tag="OO433_001782"
     CDS             22170..23099
                     /gene="tus"
                     /locus_tag="OO433_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6050288.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHCDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(23096..24499)
                     /gene="fumC"
                     /locus_tag="OO433_001783"
     CDS             complement(23096..24499)
                     /gene="fumC"
                     /locus_tag="OO433_001783"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6050289.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         5955 a         6350 c         6029 g         6331 t
ORIGIN      
        1 gaccacttca catattcacg atcagagcga acgacgcttt gtggtggaat gtgacggcga
       61 aaaagccggt ctggtggagc tggtggaaat taaccatgta catcgccggg cagaatttca
      121 gataattatc tccccggagt atcaggggaa aggtctggca acccgtgccg ccaaattagc
      181 aatggactat ggcttcaccg ttctcaatct ctataagctg tatctgatcg tcgataaaga
      241 gaatgaaaaa gcgattcaca tttaccgcaa gcttggcttt acggttgaag gtgaattgat
      301 gcatgagttt tttattaatg gtcaatatcg taatgccatt cgcatgtgta tattccagca
      361 tcagtatctg gcagagcaca aaacaccggg tcagactctc ctgaagccga ccgcacaata
      421 gcattaataa taatcgatcg tatttttgat ggtgtaaacc cgttcgacgg cgggttttac
      481 tccttcatca acaatgatca gctgacagtc caccggattg gcgtgactgt catattcaca
      541 gctctgtttt acattaccaa ctcgttgatt attcagtaaa gtaaccgctg tgtaatctaa
      601 ttttttgatc gggtccgttg atggcgtggc gctgacagat aatgttttgt cgttactttt
      661 cgtggtttta cccagtggat aaccctgatc atcatagcga tattccatct gcatttgttt
      721 gctgctggct ttaatcacga agccattatc atcggtttcc caactcaccc cggcagaagg
      781 taattctgct agctggcatt ttccctgtaa acgtactctc ttctccagcg tctcggcatc
      841 acggtaataa ttggcgtcca gtaccagagc gaccacggta ttattttcca gatccagtaa
      901 ttcgagtgaa tcaaaacagc cttcttccga caaagtccca gaaacacgtt tcgtcacttc
      961 accttgctca tccattaatg tctgagtgaa atcttttacc ggaccacgca gcggatcaaa
     1021 atcgaattca ttagagaaac tggccatctc aggggtaaat gaaagtgtta cctctgtgcg
     1081 gtcacatcct gtgaggaata tcgcgagtaa gcatggtagt aatttgtatt tcacaacagt
     1141 caccagagag tagagatgat tctcaatcat agtagcaaat acagtacttt acacgttaaa
     1201 tgctatgctt aaagaagtta tcttcgcgta aggagcttat gatgaaactc tcaacctgct
     1261 gtgccgcgct tctgctcgcc ctcgcctcac ccgcggtact cgccgcgccg ggatcctgtg
     1321 agcgcataca gagcgatata tcacagcgca ttatcaataa tggcgtaccc gaaagcagct
     1381 tcacattaag tattgtaccc aatgaccagg ttgatcagcc tgattcccag gtcgtcgggc
     1441 attgtgctaa tgatacgcat aaaattctct acacccgcac caccagcgct aacgtctctg
     1501 ctcccgcgca gtctacccag gatggcgcgc ctgccgaacc gcagtaatac attatcgact
     1561 gaacgccgga tatgacacat ccggcgattt gaacatacaa cataatccca ccttattact
     1621 catacccttc tattgatgtg gattaataat tcttaaccca aaatgggtag accccctcta
     1681 ttgttagcgc gctaaatatt caatacataa acatttatat aacgataaag aacagggagt
     1741 gagttatgtc caaaaatgac cgaatggtgg gcatcagccg cagaacactc gttaaatcaa
     1801 ccgcgatagg ttctctggcg ctggctgcag gcggtttttc tttaccgttc accctgcgca
     1861 gtgcagcggc aacggtacaa caggccagcg aaaaagtgat ctggggtgcc tgttccgtca
     1921 actgtggtag ccgctgtgca cttcgtctac atgttaaaga taatgaagtg acctgggtgg
     1981 aaactgacaa taccggcagc gatgagtacg gcaaccatca ggtacgcgcc tgtttgcgcg
     2041 gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaattaccca atgaaacgcg
     2101 tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc ctggatacta
     2161 tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaggctgta tatattcagt
     2221 actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc tcggcggtca
     2281 aacgcctgat gaactgctac ggcggttccc tcacgttgcc ggacttattc tggggctaat
     2341 cctcatactt ggcggtgaga tgatcgggcg tgtgctcttt tatggcctgc acatgacagt
     2401 aggaatggct attgcaggtt aacaaattgg tgcggggcaa cccgcacatt tcaggatgtt
     2461 aggaatgacc catttttcac agcaagataa tttttctgtc gcggcgcgcg tgttgggcgc
     2521 gctgttttat tacgctccag agagtgcgga agccgcacct ctggttgcgg tactcaccag
     2581 tgacggctgg gaaactcagt ggcctctacc agaagcgtca ttagcgccgc tggtgacggc
     2641 ttttcagaca cagtgtgaag agactcacgc ccaggcctgg cagcgtttgt ttgtcggccc
     2701 gtgggcactg ccgtctccgc cgtgggggtc ggtctggctg gatcgcgaat ctgtgctgtt
     2761 tggcgattca acattggcac ttcgtcagtg gatgcgcgag aaaggcattc agtttgaaat
     2821 gaagcaatac gaaccggaag atcattttgg ttcactgctg ttgatggctg cgtggttggc
     2881 ggagaatggt cgccagacag aatgcgaaga attgctggca tggcaccttt ttccgtggtc
     2941 aacacgtttt cttgatgttt ttatcgaaaa agcagaacac cctttctacc gtgcactggg
     3001 tgaactgtct cgcttaacgc tggcgcaatg gcaatcacaa ctgttaattc ctgtcgcggt
     3061 taaaccgctg tttcgatagg atcaccgtaa tattgccgga tggtgacgat tacgcgcaat
     3121 ccggcaataa taggttacag tgtcacgttt ttttatctct taaagcacgc actgcttttg
     3181 cggctggcct cttttgccgc aaaatagtcg cccgtgtttc attgcccatt tctgctcatg
     3241 catcatctac acatctatcc ggatctgcgc actatgttcc gccgtctgct tatcgcaaca
     3301 gtcgtcggta ttctcgcggc ctttgccgtt gccgggtttc gtcatgcgat gctgctactg
     3361 gagtggttgt tcctcaataa tgactccggc agtctggtca atgcagcgac aaacctttcc
     3421 ccctggcgac ggttgctaac tccggcgctc ggcggactgg cggcgggttt gttgctgatg
     3481 ggctggcaga aatttaccca acaacgccct catgcgccga ccgattacat ggaagcgttg
     3541 caaaccgatg gacagttcga ttacgcagca agcctggtta aatcgcttgc ctcgctgctg
     3601 gtagtaacca gcggcagtgc aattggtcgc gaaggtgcga tgattctttt agctgccctt
     3661 gccgcctcct gttttgccca acgttttacg ccacgccagg agtggaaatt atggatcgcc
     3721 tgtggcgccg cggcgggaat ggctgcggcc tatcgtgccc cgcttgctgg cagtttattt
     3781 atagccgaag tgctgtttgg cactatgatg ttggcctctc tcggcccggt gattatttcc
     3841 gccgtcgtgg cattgctggt tagcaatctg attaatcata gcgacgcgtt actctacaac
     3901 gtacaactct cagtgacggt tcaggctcgt gactatgcgc tgattatcag tacaggtgtg
     3961 ctggcaggtc tgtgcggacc actgttgtta acgttaatga acgcctgtca tcgtggattt
     4021 gtgagtctca aacttgcgcc gccctggcaa ctggcactag gcgggttgat cgtgggtctg
     4081 ctttccctgt tcacacctgc agtgtggggc aacggctata gcaccgtaca atccttttta
     4141 accgccccac cactgttaat gatcattgcc gggatcttcc tttgtaaact gtgtgccgtg
     4201 ctggcgagta gcggttccgg cgcacccggt ggtgtcttta caccgacgct atttatcggt
     4261 cttgccattg gcatgttgta tggtcgtagc ctgggattat ggttccctga tggcgaagaa
     4321 attacgcttt tactcggatt gaccgagatg gcgacactgt tggcggcaac cacgcacgcg
     4381 ccgattatgt cgacgttgat gatatgtgaa atgaccgggg agtatcagct actccccggt
     4441 ttattgattg cctgcgtcat tgcgtcggta atttcgcgga cgttacaccg tgactctatc
     4501 taccgccagc acactgcgca gcatagctaa gcggatgtat tgccccagtt ctcgctgttc
     4561 ggcgcgcggc agataaggca gttcaccaat gagcggtgcc ggaagttttt tacccagcac
     4621 atcaatgatt tccgcataat gcgccagtcc tgggttgatt cggttagcca cccagccaat
     4681 gagcggcagc ccgtcgttgg caatcgcctg agctgttagc agtgcatggt taatgcaacc
     4741 ttcctgaata ccgacaacca tcaacaccgg caactgttcc tgcaccaccc attcagagag
     4801 tggacgcaaa tcattcatca gactgcgcca gccgccagtc ccttctacca cgacatgatc
     4861 gactttatcg gtcaggtttg ccaggccgtt tgaaatgagg gtgtaattga ttgggcaact
     4921 gtgcgccacg ctactttctt cttcgcttaa cgcgatagga ttaactgctt cataaggtag
     4981 ttcgatggtt gaaacactct gcaacaccag ggcatcttta ttacgcagcc cttcgggtgt
     5041 ctctttgctc cccttcgcta cgggtttata tcccgcaact gtttttccct gggaggctaa
     5101 cgcttgtagc aatgcgcggg aaaccaccgt tttccctaca gaagtgtctg taccggtaat
     5161 aaagaaacgc ttcagcatca ctaactccac cgttatgctt cacaaatata aactaggaaa
     5221 ataattaacc ttgaaagtct aagttatgct ttcctggccc aaattgagat agcgcaaatt
     5281 ttggtagaac agttaaaaaa tgttaaccat gcaacagacg aatcaacaaa gaaccgttat
     5341 acatcgcgtc ttttaccagc gcagcgcctg ccatcgtgcc ctggttagaa aactgagtac
     5401 tctcaacgct aatgtgctga ctatacgtag gaagggcctg ctgacgtatg ctgtccgaga
     5461 tgatagggaa gaggatatct gccgctttac ttaacggtga gccaatcagt attttttgtg
     5521 ggttaaataa attcaccatg atggcaagaa tgcgcccgac atgcgcgccc accccggtaa
     5581 tgatgtcttt tgccagtaga tcgccgcgca atgccgcctg acacaatgag tccacggtta
     5641 acggttgccc atgtaacatc gagctcatgg attgattgag acgcagctgt gccagctcaa
     5701 gaatactgtc cacactggcg atggtttcga ggcagccgag gttcccgcaa taacagcgtt
     5761 taccatatgg gtcgacctgt gtgtggccta tttccacgag actactgctg cctgcgtgta
     5821 gcagatgacc atcggtaatg acgcccgccc ccacgttgtg atcgataacc acctgaatca
     5881 catcgcgcgc cccgcgtgag gcaccaaaca aggcctctgc catcgtccat gcgctgatat
     5941 catgctgaat ataaaccggt acgccggtat gctgctccag cgcctcgccg agcggcatct
     6001 cttttacatc ctcgtagaac ggcatgcgat gtacaatacc attttccgta tcaataattc
     6061 ccggcagggt tatggcaatc gaagttagac gctcaagttt tttctggtgg cggataaaaa
     6121 actgatcgat atgggaaatg atacgatcca gcaatggcaa gtcatctttt aacgccagtt
     6181 cctgcgactc ttccaccacc agtttgctgc tcagatcgcg cagagcaagg aaaatctcac
     6241 cgcgactaat gcgcagagaa agatagtgcc aggcttcagt ttcaaccacc agtcccaccg
     6301 ccggacggcc acggttcccc gcttctttga tttccagctc ttgcaccaag tgtgcttcga
     6361 gcatctcacg gacaatttta gtgatactgg caggagccag ctgcgccaga cgggaaagat
     6421 cgatacgcga gactggacca agctgatcaa tcaggcgata aaccgcgccc gcgttggtct
     6481 gctttatttg atcaatgtgc ccaggctggt tttcagcaac caccgcatac tccctatatt
     6541 ttcgcgctcc gaaataatct gtaggctatg gtgaagcact tcaatacgtg tcgtcaaatt
     6601 tttacttaga catgtgatta acagcacatt tttcgggctt tttcgctgaa atttccctca
     6661 cctgagagca ttcagcagat gaatacgaaa gttacgcgaa gctggacttt gttcatgatg
     6721 tttcggccag accaaccaca tttcagaaac cgcatcctct tcagcaatcg gcacccagcg
     6781 catttcgttg agctgagcac gtttaaatga agcaggcaaa attgaaacac ccagaccggc
     6841 ggaaaccaga ccgatgatgg tcattgcctc gcccacctcc tgagtgatga cgggcgtcaa
     6901 atggtaacgt cgcatcagcc cgagaatatc gtcatacagc cctgtcccga cgtgcggatc
     6961 aaaaaagacg aagggttctt tcgccagttc agccaacgtt acattcgggt tatttgccag
     7021 gggatgatcg tgcgggatca tcgccataag cggttcatgg acgatgactg cgtgttcaag
     7081 cgtctccggt aacgctgtgt tacgcagcaa tcccatatcc agcgttcctt caatgagcgg
     7141 agcgatttgc tcacgagtgt tcatttcgcg ggtttgtaaa tggacaccgg gataatcacg
     7201 gcgaaacagc gataacgtat cggacaccgc ccgaataaaa ggagcggacg aagtaaaacc
     7261 aatgcgcaac tcccccgctt caccctgatg cagtctttca gcgcgagcgg cagcgtcatc
     7321 taccatagac aggatttgcc gactatctgc aagaaactgt tttcctgctg ccgtcagcaa
     7381 tacactgcga ttagttcgtg ccagcagtcg ggcaccaatt tgttgctcca gtgcctgaat
     7441 ctgctgactt agcggtggtt gcgaaatatt caggcgggca gcggcgcgcc cgaaatgcag
     7501 ctcttccgca acagcaacaa agtaacgcag atgacgaagt tcaatattca tatttaaaac
     7561 atcttatttg agattattaa tatattagac agaacaattc gattttccta acctatgtat
     7621 aagcctgatc cacaggcata tttagcaagg atttcaagtg agccgtacta caactgttga
     7681 tggcgctccg gcaagcgaca ctgacaagca aagcatttct cagccaaatc aatttattaa
     7741 acgcggtacg ccgcaattta tgcgcgtcac cctggcgctg ttctctgccg gactggcaac
     7801 ctttgcactc ctctattgtg tgcagcctat ccttccggtg ctttcgcagg agtttggctt
     7861 aacccccgcg aacagtagta tttcactgtc catttccacg gcgatgttgg ctattggttt
     7921 gctgtttact ggccccctat ccgatgccat tggtcgcaaa ccagtgatgg tcacggcgct
     7981 actgttggcc tcaatttgta cgttatttcg acaatgatga ccagctggca cggcattttg
     8041 attatgcgcg ccttgattgg gctttcgtta agtggcgtgg cagctgttgg catgacttat
     8101 cttagcgagg aaatccaccc cagtttcgtg gccttttcga tggggttgta tatcagcggc
     8161 aactcaattg gcggcatgag cggacgctta attagtggtg tcttcacgga ctttttcaac
     8221 tggcgaattg ctctggcggc aatcggttgt ttcgcgctag cctcggcgtt gatgttctgg
     8281 aaaatccttc cggaatcacg ccattttcgc ccgacttcgc tgcgacctaa gacgttgttt
     8341 atcaactttc gtctgcactg gcgtgaccgg ggattaccgt tattgttcgc agaaggcttt
     8401 ttgctgatgg ggtcgttcgt cacgctgttt aattacatcg gctatcggtt gatgctttcc
     8461 ccctggcatg tcagtcaggc cgtggttggc ttattatcgc tggcttattt gaccggtaca
     8521 cggagctcac ccaaagccgg aaccatgacc acccgctacg ggcgcggtcc ggtgatgttg
     8581 ttttcgacgg gggttatgct gtttggttta ctgatgacct tattcagctc gctgtggctg
     8641 atctttgccg gaatgttact cttctcagca ggattcttcg cagcccactc agttgccagc
     8701 agctggatcg gcccccgcgc aaaacgcgct aaaggccagg cctcctcgct gtatctgttc
     8761 agttactatc tgggatcgag tattgccggg acgctgggtg atgttttctg gcataactat
     8821 ggctggaacg gcgtcggcgc atttattgct ctgatgctgg tcattgctct gctggtcggg
     8881 acgcgtttgc atcgtcgtct gcacgcctga aaaataagcc cggactgcgg taactacacg
     8941 tccggactta ttgcttgctc aaaccaacgt taatagccat cctaaaatag acgaagcgcc
     9001 agccaattcc cgcagtgcgt ctagcgtcat caggattata agtacccaaa taaacggatt
     9061 cattttgctg tgtgtcattt attactgatg cgcagttatt ctaccgcttt gtaagtagta
     9121 aaatagttaa cccgatcaag actactatta ttggtagcta aatttccctt aagtcacaat
     9181 acgttattat caatgctgta atttattcag cgtttgtaca tatcgttaca cgctgaaacc
     9241 aaccactcac ggaagtctgc cattcccagg gatatagtta tttcaacggc cccgcagtgg
     9301 ggttaaatga aaaaacaaat tgagggtatg acaatgaaaa aagtattagc tctggttgtt
     9361 gccgctgcta tgggtctgtc ttctgccgcc tttgctgcag agactgcgac gaccaccaaa
     9421 gcagcgccgg cgaaaacgac acatcataaa aaacagcata aagcagcacc tgcccagaaa
     9481 gcgcaggcag ctaaaaagca tcataaaaat acgaaagctg aacagaaagc ccctgaacaa
     9541 aaagcgcagg cagcgaagaa acacgccggg aaacacagtc atcagcaacc ggcaaaacct
     9601 gctgcacaac ccgcagcgta agttttcaac agtaatgctg gcgcgccccc tcgcgcctga
     9661 aaattacggt gctaagcggg taacgtttag caccgccttt agcggagggt agtatggtgg
     9721 gccgttatcg ctttgagttc attcttatca tccttatttt atgcgcactg attgccgccc
     9781 gtttttatct ttcctgactg tagttatctg attttactcc cactttcatc ccgtcccgtc
     9841 tatagtattt atgagggttt gcttttaata atcataatta cccaccagag tgtgatatgc
     9901 gtacaaccat tgctgtagtg ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag
     9961 ataaaccaga cgttgccaaa tcggcaaacg atgaggtcag caccctgttt tttggtcatg
    10021 atgatcgtgt gccagtgaat gacacggccc aatcaccgtg ggatgcggtt gggcaactgg
    10081 aaacggccag cggcaattta tgtacggcga cgctgattgc acccaatctg gcattaacgg
    10141 caggacactg tttattgaca cctccaaagg gtaaagccga taaagcagtg gtgctgcgtt
    10201 ttgtgtcaaa taaaggtctt tggcgctatg agatccacga catagaaggc cgcgttgatc
    10261 cgacactggg aaagcggtta aaagcagatg gggatggttg gattgtacct cccgcagccg
    10321 cgccgtggga cttcggattg attgtgctac gtaatccccc ttctggcatt acaccgttgc
    10381 cgttatttga gggagataaa gccgcgctta ctgccgcatt aaaatcggct ggccgtaaag
    10441 taactcaggc gggataccct gaagatcatc tcgatacgtt gtacagtcat caaaactgtg
    10501 aagtgactgg ctgggcgcaa acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg
    10561 acagcggttc gcctctgatg ttgcatacca atgacggctg gcaattaatt ggggtgcaaa
    10621 gttcggctcc tgccgcgaaa gatcgctggc gcgccgataa ccgggccatt tctgttaccg
    10681 gttttcgcga caagctggat caactgtcgc aaaaataatg ttcaaacgct gcccgactgt
    10741 gcgggcagcg tgttcatcag gcaagtttca ccatgatcat tccagccaac agcaagacca
    10801 ggccaatcca gcctttacga tttaaccgtt gaccaaacaa gatccaacct gcggctaacg
    10861 tggcggcaat accaaacccg ccccacaatg cataagcgac agacaagtcg atccctttaa
    10921 cggcctgaga aagcgcacta aaggcagcca gcaccgccgc cagggagagc aagccaaata
    10981 ttttgcgacg aaagccgtca gaaaatttca aaaagacgtt agcaacgatt tccagcacga
    11041 ttgccaatgc cagccaggcg gcgtgaaccc attcaaactg cgccatggtt tacctccagt
    11101 tcaggtttac gcgctttacg ggtacctgat tttatcaaca caatcccggc gaccagggtg
    11161 gttaacccgg caattttcat cagcgataaa ctttcgtcga ataacaaaac gctaaacaag
    11221 gtaataaata aaataccgat accttcccac aacgcataag ctacgcctaa ggcgatcttt
    11281 ttaacggcga aagagagaaa tatatacgac agagaaatca tcaccagcat taaaataaag
    11341 ccgccatttc cctcactgac gctcgcccat ttcattgaca gcgtaccggt aatttctgta
    11401 gcaatagcca ggcctaataa aatccaataa atatacattg tccttctcct gcaagagaat
    11461 tattttaatt ttcgcttaat tcagcgaaat caaagtaaag tggtcaagcc ccgcgcatag
    11521 ggcaagctta ggcagaagaa aggactaaag cgcgttgcgc cagtgctgct cacctacgag
    11581 caagatagtg gatgaggtac aaagatgggt aaatgttgaa aataaggtcc tgaacaaatt
    11641 gtccataata ttacaattat ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc
    11701 tcggtagttg aacacgcctg atttgtatca tagcttaaga attaactcaa aatattttca
    11761 cttctttacc tgagcggttt gattttcgtt atgatgacgg agcgaaaaag acattattat
    11821 tagcaaagga agaaaaaacg gggacaagca tggcaaagcc gatcatcacg ctcaatggcc
    11881 taaaaatcgt cattatgttg ggaatgctgg tcattattct ctgcggtatc cgttttgccg
    11941 ccgagatcat cgtgccgttt attctcgcat tatttattgc tgttattctt aacccgctgg
    12001 tgcaacacat ggtccgctgg cgagtgccgc gtgtactggc ggtgtcgatt ttgatgacca
    12061 tcatcgtgat ggcgatggtg ttgctattag cttatctggg ttccgcgctc aacgagttga
    12121 cgcggacgtt accgcaatat cgcaactcta ttatgacgcc gctgcaagct cttgagccgt
    12181 tgttgcaacg cgtagggatt gacgtctcag ttgaccagct ggcgcattac attgatccga
    12241 acgcagcgat gacgttgctc accaacttat tgacgcagtt atctaatgcc atgtcatcaa
    12301 tatttttatt gctgctgacg gtgctgttta tgctgctcga agtgccacaa ttgcccggaa
    12361 aatttcagca aatgatggcg cgtccggttg aagggatggc ggcgattcaa cgtgcaattg
    12421 acagtgtttc tcattatctg gtgctgaaaa cagccatcag catcatcacc ggcctggtcg
    12481 cctgggcgat gctcgccgca ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct
    12541 ttgcgcttaa ttacatccct aatattggtt cagttctcgc ggcaatcccc cctatcgctc
    12601 aggtactggt gtttaatggc ttctacgaag cgttgctggt gctggcggga tatctgctga
    12661 ttaatctggt cttcggcaat attctggagc cgcgcatcat gggacgtggg ctggggcttt
    12721 ccacattggt ggtatttttg tcgttgattt tttggggatg gttgttagga ccggtgggta
    12781 tgctgctttc cgtgccgtta acaattattg tcaaaattgc gcttgaacaa acagcgggag
    12841 gtcaaagcat cgccgttctg ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc
    12901 cgtcagggtt acagagcttt caggattgca tccacgctgg ctttggcgtc accaaacagc
    12961 atgtgggtgt tttccttgaa gaacagcggg ttttgcacac cagcatagcc agtgttcatc
    13021 gaacgtttaa agacaatcac gtcctgcgct ttccacactt ccagcacagg cataccagca
    13081 ataggactct tcggatcatc ctgcgccgcc gggttaaccg tatcgttagc accaatcacc
    13141 agtacggtat cggtatcagc aaagtcatcg ttgatctcgt ccatttccag cacgatgtca
    13201 tacggtactt ttgcttcagc cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc
    13261 gggtggatac cgaaacgcac gttgatacca cgagcgcgca gtttctcggt aatttcagcg
    13321 acaggatatt gcgcctgcgc gactgccatg ccgtaccccg gagtaatgat cactgaatgg
    13381 gagtttttca gcagttccgc tgtctcttct gcggtgattt cgcggtgctc gcccacttcc
    13441 tgatcatcgc cagtagaaga gccgtcggtg ccgaaaccac ccgcaataac gctgataaag
    13501 gaacggttca tcgccttaca cataatgtaa gaaaggatag cccccgaaga accgaccagc
    13561 gcaccggtca caatcagcag gtcgttgctg agcataaagc ccgcagccgc agccgcccag
    13621 ccggagtacg agttcagcat cgacaccacc actggcatat ctgcaccacc gatggaggcg
    13681 actaaatgcc agccgaatac cagcgcaatg gcggtcatta ttagcaatgc cagcacttgc
    13741 aggccgacgc tgtccgtgcg aacaaataca atcagcagca ggaaggaaac gaccagagcc
    13801 gccaggttca ttttgtgacg gtttggcagc atcaacggtt tagacgaaat cttgccacac
    13861 agtttgccga acgccaccac cgaacccgtg aacgttaccg ccccgatgaa gataccgagg
    13921 aacacttccg tcaggtgaat attgaccaga atcggtgcca ttcccgcgtc atgatgcaga
    13981 tagctgttaa agccaaccag cactgccgcc agacccacga agctgtgcag gatcgccacc
    14041 agttctggca tttcggtcat ttcaactttc ttcgccagac ggataccaat tgccccacca
    14101 atgaccatcg ccagcaagat ccagccaaca ttacccgtat ccggtccaaa aatggttgcg
    14161 attaacgcaa tcgccatccc tgcgataccg aagttgttac cctggcgaga cgtttcatgt
    14221 ttcgaaagac cggccagact gaagataaac aggatcgcgg caacaatgta tgcagctgta
    14281 actaatcctc cagacatatg ttacccctta atttttgcgg aacattttca gcatgcgctg
    14341 agtcacggtg aagccaccga aaatattaat gctggctata agcaccgcga taaaactaag
    14401 gaagctaacc cagccgccct ggccaatctg caacagtgct ccgacaacaa taatccctga
    14461 aatcgcgttg gtgaccgaca tcaacggtgt atgcagcgcg tgcgatacat tccacaccac
    14521 gtaataaccg acaacgcagg ccagcgcgaa aacggtgaag tgcccaagaa actctttcgg
    14581 cgcaacgctt gccagccaac cgaaaaggat gattgccagc gccatcaacg cgtatttacg
    14641 ccacggtgag caggcacatt tttcctcagt tttcacttcc ggtgccgctt tttgtgccgc
    14701 ctgcggctga gctgatacct gaatcggcgg tgccggccag gtaatttcgc ccgcacggat
    14761 cacggtcacg cggcgaatca ccacatcatc aaaatcaaca gtgatattgc cgtctttctc
    14821 tttgcacaac agtttcagca gattaacgag gttagtaccg taaagctgcg aggattgcgt
    14881 cggcagacgg cccggaagat cggtataacc aatcactttg acaccatttt ccgtagtgaa
    14941 gatttcaccc ggcacggtgt attcacagtt gccaccgttt tgggctgcca ggtcgacaat
    15001 cacactgccc gtcttcatgg agtcaaccat ttcacgggta attagcttcg gcgctggttt
    15061 gcctggaata agcgcggtgg tgacaatgat atcgacctct tttgcctggg cggcaaagag
    15121 ttccatttct gctttaatga acgcgtccga catcactttg gcatagccat cgccgctgcc
    15181 cgcttcctct ttaaaatcca gctcgaggaa ttccgcgccc atactttgaa cttgttcttt
    15241 cacttccgga cgggtgtcga atgcacgcac aatcgcgccg agactgtttg ctgcgccaat
    15301 ggcggccaga cctgcaacac ccgcaccaat caccatcact tttgccggtg gcactttccc
    15361 ggccgcagta atttgcccgg taaagaagcg cccaaattca tgtgccgctt caacaatggc
    15421 gcgataaccg gcgatgttcg ccatcgagct tagtgcgtcc agcgattgtg cgcgtgagat
    15481 acgcggcaca gaatccatcg ccatcacggt cacgttacgt tccgcaagtt tttgcattaa
    15541 ttccggattc tgcgcaggcc agataaaact caccagcgtt gtcccaggat tcagtaacgc
    15601 aatttcatca tctaacggcg cattgacctt cagaatgatc tctgactgcc agacgctatt
    15661 cccttctaca atttcagcgc ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg
    15721 acccgcgccg ctctctaccg cgacggtaaa acccagtttc agcagctgtt ccactgtttt
    15781 tggcgttgct gcaacacggg tttcattggt taaccgttct cttggtatgc caattcgcat
    15841 gatattccct tccatcggtt ttattgatga tggtttgcct gtgtcaggag ccacacaagc
    15901 cgctcatgta cgagctaaat gttactccgt taaaataaat tagtaacaaa cgccctataa
    15961 cgtactgaaa attatgcctg tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc
    16021 aaaatattaa cgattcagcc gtgatagtgg gataaacacc ttagaacgcc ggataaagac
    16081 tgataattgt cttcgacggt cgggtaaaac gagacatcgc cccggcacga ttcactactt
    16141 aacattaaat taacttatac aattcagttg cttcagtagt aatgatgctg atacggctgt
    16201 tttttaagca tagacggtca tttgagcagg attaaaattg gcttaaggaa tgtgatatga
    16261 aaaatgacgc agacagttac accgtttaaa tgcaataatc agccacgttt ctcgttaata
    16321 acaataccag tacctggttt gcgcaaggcg aaggattatt tttatgaagc ttaagaacac
    16381 cctcctggcg tcggcactgc tttctgctac ggcattctcc gttaacgcag caacagaact
    16441 gacaccggag caagcggcag cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt
    16501 taatgctatt ggcgaagcgg tgaaagccgt ttctcgtcgc gcagataaag aaggtgccgc
    16561 ctctttttat gttgtcgaca cttctgattt tggtaacagc ggtaactggc gtgtggtcgc
    16621 tgacctctat aaagccgatg ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg
    16681 tgttgtcgaa ctgccgaaag atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt
    16741 ccagggcttc tatcgtagcc agccagaagt caatgatgcc atcaccaaag cggcaaaagc
    16801 gaaaggtgcc tactctttct acatcgttcg tcaaatcgat gccaaccagg gcggcaacca
    16861 gcgtattact gcattcatct ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga
    16921 tgtgatcccg gcagattccg aagcaggacg tgcagctctg gctgccggtg gcgaagccgc
    16981 gaagaaagtt gagatcccag gtgttgcgac taccgcatca ccaagttctg aagtcggtcg
    17041 tttctttgaa acccagtcat caaaaggcgg gcgttacacc gtcacgctcc cggatggcac
    17101 taaagtcgaa gaactgaaca aagcgaccgc agcgatgatg gtcccgttcg atagcatcaa
    17161 attctctggc aactacggca acatgaccga agtctcctat caggttgcga aacgtgccgc
    17221 gaagaaaggt gctaagtatt accacatcac ccgccagtgg caggaacgtg gtaataacct
    17281 gaccgtcagc gcagatctgt ataaataact attcagttac tcctataagg cggcttgatg
    17341 ccgcctttcg catttttagt cacaaatttc cccgcgacca ttgcatgcag acacatctct
    17401 tcgtaaaatc ccgcgcttca gtggcaaact ccatttttat gcgttttgcc ctaatattta
    17461 ttcttcttat cacgttttaa tcactggata tcgatggaaa agaaactggg actgagcgca
    17521 ctcaccgcgc tggtattaag ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat
    17581 atggcggcag ttgccagccc ggcagcactg ctcatcggct ggggtattac tggcgctggc
    17641 attttattgc tggcctttgc catgctgatc ctcacgcgca ttcgtcccga acttgacggc
    17701 ggtatcttta cctatgcccg cgaagggttt ggcgagctaa tcggcttttg ttccgcatgg
    17761 ggatactggc tgtgcgcagt catcgccaac gtctcctatc tggtgatcgt tttttccgcg
    17821 ttaagctttt ttacggacac gccggaattg cgcctgttcg gcgatggtaa tacctggcaa
    17881 tcgatcgtcg gtgcatcggc tttattgtgg tttgttcact ttttgatcct gcgcggggtg
    17941 caaaccgctg ccagcattaa cctggtggcg acactggcaa aattgttgcc gctgggtctg
    18001 tttgttgtgc tggcgatgat gatgttcaaa ctggatacct tcaagctcga cttcaccgga
    18061 cttgtacttg gcgtacccgt ttgggaacag gtgaaaaaca ccatgctgat caccctgtgg
    18121 gttttcattg gtgtggaagg tgccgtggtt gtttctgcgc gtgcgcgtaa taaacgtgat
    18181 gttggcaaag cgacactgct ggcggttctc tccgctctgg gcgtttactt gctggtaacg
    18241 ctgctttcgc tgggtgtggt ggcacgtcct gaactggctg aaattcgtaa cccgtcaatg
    18301 gccggtctga tggtggaaat gatgggacca tggggcgaaa tcatcatcgc tgccagtttg
    18361 atcgtttccg tttgcggcgc gtacttaagc tggaccatca tggcggcaga agttccgttc
    18421 ctggctgcca ctcataaagc attcccacgc atttttgcgc gccagaacgc acaagctgcg
    18481 ccatccgcct cactgtggtt gaccaacatc tgcgtgcaaa tttgtctggt actcatctgg
    18541 ctgaccggtt ccgattacaa cacgttgctg accatcgcct cagaaatgat tctggtgccc
    18601 tatttcctgg tcggcgcgtt cttgctgaaa atcgcaaccc gtccactgca taaagcggta
    18661 ggtgtcggtg cctgcattta tggcttatgg ttattgtacg cctctggtcc aatgcacttg
    18721 ctgttatccg ttgttctgta tgcgccgggg ctgctggttt tcctgtatgc gcgcaaaacg
    18781 catacacatg ataatgtact gaaccgtcag gaaatggtgc tgattggcat gctgttaatt
    18841 gcctcagttc cggcgacctg gatgctggtg ggataacgtt catcccatcg ttggatagca
    18901 aaggagataa cgatgggtaa agcccagccc ttgccaatat taattactgg cggaggtcgt
    18961 cgcatcggcc tcgccctcgc ctggcatttc attaatcaaa agcaaccggt gattgtcagc
    19021 tatcggacgc actatccagc cattgatgga ctgattaagg caggtgcgca gtgtattcag
    19081 gctgattttt cgaccaacga cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc
    19141 catggtctgc gtgctatttt gcataacgcc agtgcgtgga tggcggaaaa actgggtgcg
    19201 ccactggccg acgtactggc ttgcatgatg cagatccacg ttaatacccc atacctgctc
    19261 aaccatgcgc tggaaagatt actgcgtggg cacggacacg ccgccagcga tatcattcac
    19321 tttaccgatt atgtggtgga gcgcggtagc gacaagcata ttgcgtatgc tgcaagcaaa
    19381 gcggcactgg ataatatgac ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg
    19441 aattctattg ctccatcgct gatcctgttt aatgaacatg atgatgccga atatcgacaa
    19501 caggcgctga ataaatcact gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg
    19561 gtcgattact tacttaccag ttgctttgtc accggacgca gtttcccact tgatggcggt
    19621 cgtcatctgc gttaatgcag ctttatccag catataatca acacccatgc aacacgcaac
    19681 agaaccaaca aaagcggcgg gcaagcgcat cattccggtg aaataccaca gcgacaccag
    19741 gtaaacaaaa tagggaataa tcgaccacat actaaaaatg atggttgcgc gtaaggcttc
    19801 aatgccgcgt tcgctggcaa caatataatg cgcgataagc gcaaaggtcg ggaaaagtgg
    19861 aatcagcccg gcgatataat aatttttcgt ttttgctaaa acaccaatca acagcactac
    19921 cagcgcaccg agcgcggctt tgattttacc ttaacacttc cataacaagt catcagtaga
    19981 atacctgatg aaaacttgtt tagaaacgat tgatagtaag taaaaacagc gcggtgtatt
    20041 gtgacgtttt tatatctacc gtgaatgtta tgaacactat cgtatttgtg gaagatgatg
    20101 cggaagtcgg ttcactgatt gccgcgtacc tggcaaaaca tgatatgcag gttaccgtag
    20161 agccgcgcgg cgaccaggcc gaagaaacca ttttgcgaga aaatccggat ttggtgttac
    20221 tcgacatcat gctaccaggc aaggacggca ttaccatttg tcgtgattta cgcgcaaagt
    20281 ggtctggacc gattgttctt ctaacctctc tcgatagcga tatgaaccac atcctggcac
    20341 tggaaatggg tgcctgcgac tatattctca aaacgatgcc ccctgctgtt ttgctagcgc
    20401 gtttacgttt gcatttgcgt cagaatgagc aagccacact gaccaaaggt cttcaggaaa
    20461 cgtctctgac tccctacaaa gccctgcatt tcggcacgtt gaccatcgat cccatcaacc
    20521 gcgtagtcac cctggctaac actgaaatct cgctctcgac agctgatttc gaattattgt
    20581 gggaattagc tacccatgcc gggcaaatca tggaccgcga tgcattgctg aaaaatttac
    20641 gcggcgtcag ttatgacgga ctggatcgta acgtggacgt ggctatttcg cggttaagaa
    20701 aaaaactgct cgataacgcc gcagaacctt atcgcattaa aactgtgcgt aacaaaggct
    20761 atctttttgc gcctcatgca tgggaataag cgatgaaaaa actgtttatc cagttttacc
    20821 tgttattgtt tgtctgcttc cttgtgatgt ctctgctggt tgggttggtg tacaaattta
    20881 ccgccgaacg cgcgggcaaa cagtcgctgg atgatttgat gaacagttcg ctgtatctga
    20941 tgcgcagcga attgcgtgag atccccccac acgactgggg taagacgctg aaagagatgg
    21001 atttaaatct ctctttcgat ctgcgtgtcg agccactgag taaataccat cttgatgata
    21061 tttccatgca ccgactgcgt ggcggcgaaa ttgtcgccct ggacgatcag tacacgtttt
    21121 tgcagcgtat cccgcgcagc cactacgtgc tggcagttgg tcctgttcct tatctttatt
    21181 atctccatca gatgcgattg ctggatatcg ccctgatcgc ttttattgct atttccctcg
    21241 cctttccggt gtttatctgg atgcgtccgc actggcagga tatgttaaaa ctggaagcag
    21301 cggcgcaacg atttggcgat gggcatctct atgagcgtat ccactttgat gagggttcga
    21361 gctttgaacg acttggtatc gcatttaacc agatggcgga caatatcaac gccttgattg
    21421 ccagcaaaaa acagcttatt gacggtatcg ctcacgaact gcgaacaccg ttagtgcgcc
    21481 tgcgttatcg actggagatg agcgataacc tgagcgccgc cgagtcccag gcgttgaatc
    21541 gtgatatcag tcaacttgaa gctttaattg aagagctgct gacttatgcc cgactcgatc
    21601 gcccacaaaa cgagcttcat cttagcgaac cagacctgcc gttgtggctg tcaacgcatc
    21661 tggcagatat tcaggcagta acgcccgata aaacggtacg gataaaaacg ctcgtgcaag
    21721 gccattatgc ggcgttggat atgcgcttaa tggagcgcgt gctggataat ttgctcaata
    21781 acgccctgcg gtactgccat tcaacggttg aaaccagcct gctactgtcg gggaatagag
    21841 cgacattaat tgttgaggat gatggcccag ggattgcccc agaaaaccgc gaacatatct
    21901 ttgaaccttt tgttcgcctc gatcccagcc gggatcgctc aaccggcggc tgcgggctgg
    21961 ggctggcaat tgtccactct atagcactgg caatgggcgg tacggttaat tgtgacacca
    22021 gcgaactggg tggtgctcgc ttctcgttta gctggccgtt atggcataac atcccgcaat
    22081 ttacctctgc ctgacactac gcgcacgatg gtcaagtcac caccattgtg ctataaatta
    22141 aggatgttgt aactaaagtg gttaatatta tggcgcgtta cgatctcgta gaccgactca
    22201 acactacctt tcgccagatg gaacaagagc tggctgcatt tgccgctcat cttgagcaac
    22261 acaagctatt ggttgcccgc gtgttctctt tgccggaggt aaaaaaagag gatgagcata
    22321 atccgcttaa tcgtattgag gtgaaacaac atctcggcaa cgacgcgcag tcgctggcgt
    22381 tgcgtcattt ccgccattta tttattcaac aacagtccga aaatcgcagc agcaaagccg
    22441 ctgtccgtct gcctggcgtg ttgtgttacc aggtcgataa cctttcgcaa gcagcgttgg
    22501 tcagtcatat tcagcacatc aataaactca agaccacgtt cgagcatatc gtcacggttg
    22561 agtcagaact ccccaccgcg gcacgttttg aatgggtgca tcgtcatttg ccggggctga
    22621 tcacccttaa tgcttaccgc tcgctcaccg ttctgcacga ccccgccact ttacgttttg
    22681 gctgggctaa taaacatatc attaaaaatt tgcattgtga tgaagtcctg gcacagctgg
    22741 aaaaaagcct gaaatcacca cgcagtgtcg caccgtggac gcgcgaggag tggcaaagaa
    22801 aactggagcg agagtatcag gatatcgctg ccctgccaca gaacgcgaag ttaaaaatca
    22861 aacgtccggt gaaggtgcag ccgattgccc gcgtctggta caaaggagat caaaaacaag
    22921 tgcaacacgc ctgccctaca ccgctgattg cactgattaa tcgggataat ggtgcgggcg
    22981 tgccggacgt tggtgagttg ttaaattacg atgccgacaa tgtgcagcac cgttataaac
    23041 ctcaggcgca gccgcttcgt ttgatcattc cacggctgca cctgtatgtt gcagattaac
    23101 gcccggcttt catactgccg accatctgtt ctggccgtac ccagctgtca aactcggctt
    23161 cgctaagata ccccagcgca agggccgcag cttttaaggt cagcccttct ttatgcgctt
    23221 ttttggcgat ctccgcggct ttgtcataac caatgtgggt gttaagcgca gtcaccagca
    23281 tcagcgattc attgagtaat tgattgattc gctcacgatt cggttcaata cccactgcgc
    23341 agtgtttgtt aaaactttcc atgccatctg ccagcaagcg caccgattgc aggaaattat
    23401 ggatcaccat cggacggaag acgttcagtt caaagttacc ggaagcgcca cccatgttga
    23461 tcgccacgtc gttccccatt acctgacagc agagcatggt taatgcttca cactgggttg
    23521 ggttcacttt ccccggcatg attgagctgc ccggctcatt ttccgggatt gagatttcac
    23581 caattccgca gcgcgggcca gaggccagcc agcggacatc attggcgatt ttcatcagtg
    23641 acgcagccaa ccctttcaac gcgccgtgcg cctgaaccag ggcatcacag gtcgcaagcg
    23701 cttcaaattt gttcggcgcg gtaacaaacg gtgcacaggt aatgactgcc agttcatctg
    23761 ctacgcgacg cgcatactcc ggatgggtat ttagtccagt acccaccgct gtaccgccaa
    23821 gagccagttc cgctacgtga ggcaggctgt attcgatatg tttgagatta tgctcgagca
    23881 tcgctaccca gccggaaatc tcctgcccta gtgttagcgg cgtggcgtcc tgcaagtggg
    23941 ttcgaccgat tttgacgata tcggcaaatg cacgcgattt ttcactcagt gtctgcgtca
    24001 gggttttaag ctgcggaatg agttgcttgc gcagcgccag cagcgccgca acgtgcatcg
    24061 ccgtcggaaa gacatcgttg gaactttggc ttttgttcac gtcgtcgtta gggtgaactt
    24121 tacgttccat cccgcgcacg ccgccgagta attcactggc ccggttagcc agcacttcgt
    24181 tcatgttcat gttactttgc gtgccggagc cggtctgcca gatagccagc gggaattcgt
    24241 cgtcatgctg tcctgccagt acttcatccg ccgcctgccg aatggcgctc gctttctctt
    24301 cagacaacaa gcctaaatct tcattaactt ttgccgctgc gcgcttggtt agcgccagcg
    24361 catgaatcag tgaggtgggc attttctccg tcgaaatgcg gaaatgctcc agcgagcgtt
    24421 gagtttgtgc gccccacagc ttatctgccg ggacatcaat cgcccccatc gaatcttttt
    24481 cgctgcgtac tgtattcatg gcctgctcct cacctgttta attttttctt tctgttttgc
    24541 tttcgctaag caacttttag ctcacttatt atttactatt tgataacaag tgtttggtct
    24601 ttcgtgccat gtaaaaaaac cgccccgaag ggcggtaatg ccagtcagtt aagcaactga
    24661 ctggc
//
LOCUS       DAJTSL010000049        24554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTSL010000049 DAJTSL010000000
VERSION     DAJTSL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.049"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(144..2999)
                     /gene="valS"
                     /locus_tag="OO433_001784"
     CDS             complement(144..2999)
                     /gene="valS"
                     /locus_tag="OO433_001784"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6050290.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVSL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(2999..3442)
                     /gene="holC"
                     /locus_tag="OO433_001785"
     CDS             complement(2999..3442)
                     /gene="holC"
                     /locus_tag="OO433_001785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6050291.1"
                     /translation="MKNATFYLLDNDTTVDGLSTVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3602..5113)
                     /gene="pepA"
                     /locus_tag="OO433_001786"
     CDS             complement(3602..5113)
                     /gene="pepA"
                     /locus_tag="OO433_001786"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6050292.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAILHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5380..6480
                     /gene="lptF"
                     /locus_tag="OO433_001787"
     CDS             5380..6480
                     /gene="lptF"
                     /locus_tag="OO433_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6050293.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6480..7562
                     /gene="lptG"
                     /locus_tag="OO433_001788"
     CDS             6480..7562
                     /gene="lptG"
                     /locus_tag="OO433_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6050294.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQTFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7723..9225)
                     /locus_tag="OO433_001789"
     CDS             complement(7723..9225)
                     /locus_tag="OO433_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6050295.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEKQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTKQQNNPPAKGKAVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(9355..10374)
                     /gene="ahr"
                     /locus_tag="OO433_001790"
     CDS             complement(9355..10374)
                     /gene="ahr"
                     /locus_tag="OO433_001790"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6050296.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDTC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            10570..10654
                     /locus_tag="OO433_001791"
     tRNA            10570..10654
                     /locus_tag="OO433_001791"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10604..10606,aa:Leu,seq:caa)
     gene            10818..12083
                     /locus_tag="OO433_001792"
     CDS             10818..12083
                     /locus_tag="OO433_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6050297.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDQAKKLLAQGIDPKAEQKEAQAENSGAYSFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYR
                     GMKMKEEHIVQLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSEDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MVQWWADYLQANKGKTITPYDFAKINKSR"
     gene            complement(12138..13148)
                     /locus_tag="OO433_001793"
     CDS             complement(12138..13148)
                     /locus_tag="OO433_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050298.1"
                     /translation="MIYITSESKETLMIKYEGKMKKNIRVIPKNIHSKLRRLGNTVVA
                     GTSIAFTENQLKSGALEHLGIYFDNGVNAYETSVIPDPLQGKYSLKNVFGEEIVRKDL
                     PKETHYTEIESPNWGDSSNGTHTVRLPYEKYPRDIIPPTLIAIEINHKQPSDSHFIFN
                     FRVTRILDKQSNKFDDELLFDLNLLQENLGKCGIEDADKPISTYADTLIVSWEIFPPG
                     SKEETLARIFRGKNITSDKKNVAENRYDFFMSLEPKKIVTGNSTFSNYIGAMLEDDLV
                     VFENIEYGNAIYILYDNWDDISKLSRIDLLSGRAGSNFDRIIHSGNWKDEVRKKVAAG
                     RL"
     gene            complement(13099..13635)
                     /locus_tag="OO433_001794"
     CDS             complement(13099..13635)
                     /locus_tag="OO433_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050299.1"
                     /translation="MLKAIAGIAKYTWIYMAIFIISLMIISSSDINYVYNDFANYCTL
                     LVSVSGMVFTIMGIWLAFLYPNALQRIVNPKTIETVDFTEQLEDTRRLEAIVGSVLKS
                     SFVMITLLVVYLVKLVFFKSHLYLESQDVIKNTSLALIIAISYMQIEAVISVILANVM
                     FINDLHNKREQRDIDDQV"
     gene            complement(13636..14715)
                     /locus_tag="OO433_001795"
     CDS             complement(13636..14715)
                     /locus_tag="OO433_001795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050300.1"
                     /translation="MNVGHLNFFKVNKCGLYKVNDDNTYGLELSETFDLIQDWVGTKS
                     LALTIPWDPKEKPNSSKCYCKDIYKDENTGDFLVMLWKSDTDSTGSLLGASEDGEIGS
                     SSVVKYTNSYRGKKVIWGRPCFYWVIPELETIVSIKFDHSVCDSELFQDYVHSSITNR
                     VKHSKRVKNKTEKGYIRLSNTDDEDLYKYMYRFDMKLRSLETTHTELGKLIPKITHIV
                     RRETIIINPNDARADWLKTFSTLVPFVSGKKNAKTRQIEIKAEAKPSLNEVQEIIEKY
                     SAEDREKRLWDNVGFATDKGITWVDKYRMRDILNVPSDDYSTYSAAYIYEQISHKRKE
                     FISPILKELKALQSQPRIKKASGED"
     gene            complement(15113..15685)
                     /locus_tag="OO433_001796"
     CDS             complement(15113..15685)
                     /locus_tag="OO433_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HCR6050301.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAT
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(15759..16259)
                     /locus_tag="OO433_001797"
     CDS             complement(15759..16259)
                     /locus_tag="OO433_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR6050302.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(16256..16990)
                     /locus_tag="OO433_001798"
     CDS             complement(16256..16990)
                     /locus_tag="OO433_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HCR6050303.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            17542..17808
                     /locus_tag="OO433_001799"
     CDS             17542..17808
                     /locus_tag="OO433_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6050304.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            17805..18404
                     /locus_tag="OO433_001800"
     CDS             17805..18404
                     /locus_tag="OO433_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6050305.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            18397..18684
                     /locus_tag="OO433_001801"
     CDS             18397..18684
                     /locus_tag="OO433_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HCR6050306.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            18677..19132
                     /locus_tag="OO433_001802"
     CDS             18677..19132
                     /locus_tag="OO433_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050307.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYQDIYPQDTVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            19237..19590
                     /locus_tag="OO433_001803"
     CDS             19237..19590
                     /locus_tag="OO433_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HCR6050308.1"
                     /translation="MQSSVTGRKANENTLLPPVLRAALYRRAVACAWLTVCERQHRYP
                     HLTLESLEAAIAAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVV
                     MDELCARHIKAPVLH"
     gene            19605..21938
                     /locus_tag="OO433_001804"
     CDS             19605..21938
                     /locus_tag="OO433_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR6050309.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDVAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEVTRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPLDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            22104..22298
                     /locus_tag="OO433_001805"
     CDS             22104..22298
                     /locus_tag="OO433_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6050310.1"
                     /translation="MWIKDALVWIRATDPNLLIKNYLTFRFIDGLKIDWSEKQWGHKR
                     GHISLLCFIICFLSLTYFDI"
     gene            22516..23784
                     /locus_tag="OO433_001806"
     CDS             22516..23784
                     /locus_tag="OO433_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6050311.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPALELKRIPELLQKID
                     EYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     AKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANREKGITPFDYAKINRGNGE"
     gene            24175..>24554
                     /locus_tag="OO433_001807"
     CDS             24175..>24554
                     /locus_tag="OO433_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050312.1"
                     /translation="MNFKKKLEEHFKQFEASPVLFVGSGVSRRYLGVPCWQDLLKHFA
                     EAIGENHIKLKTKSNGDLPEYAQLLVSAYAEKWWDTEEGQLALSEKEQEKTFINEQSP
                     LKLSISKYIENAHKNIIDNDELKH"
BASE COUNT         5831 a         6365 c         6100 g         6258 t
ORIGIN      
        1 tcctttaatc aataaagaag aacaaccaga taaaggcttc ttagccagta tttcacgggg
       61 aattatgcct tctttaccat ttctgtaaga gataaaaaag gccggagcat gctccggcct
      121 tcgttttcat cactgtgttt tgattacagc gcggcgataa cagcctgctg ttcaatcagc
      181 ttcgctttcg cttctgcata gccttccagc ttctcacgct ctttcgcgat gaccgcttcc
      241 ggtgcgcggg cgacaaagcc ttcattcgcc agcttgttct cgatacggct gatttcgcct
      301 tcgatcttcg ccacttcttt cgccagacgc gccagctcat cttctttgtt gatgaggcca
      361 gccatcggga tcagcagctc tgcgccgtcg atgatcttcg taacggaaac cggacctttg
      421 tcatcggcag gcagcacggt gatgctttcc agacgcgcca gggtttgcag gaagccacgg
      481 ttttcattta cgcgacgttc tgcatccgcg ctgcaaccac gcagcagcag ctccagcggt
      541 ttgcccggcg cgatgttcat ttctgcacgg atgttacgta ccgcaacgat cgcctgtttc
      601 agccactcgg tgtcagccag tgcggcttca tcaacctgag atgcatcgta ctgcgggaac
      661 ggctgcagca tgatggtgtc ggcagtgata ccgcaaagta ctttcacacg ctgccagatg
      721 gtttcggtga tgaacggaat gatcggatgc gcgaggcgca gcagaccttc cagtacagtc
      781 accagcgtat ggcgagtacc gcgcagttcg gcttcggttc caccgttcat taccggctta
      841 gtcagctcca gataccagtc gcagaactgg ttccaggtga actcatacag aatgcctgca
      901 gcgatatcga agcggaagct gtccagcgct tcgcggtacg ctttgatggt ctggttgaac
      961 tctgccagaa tccagcggtc cgccagcgac agcgtcattt caccaccgtt gaagccgcaa
     1021 tcctgacctt ccgtgttcat cagcacaaag cggctggcgt tccacagctt gttacagaag
     1081 ttacggtagc cttccaggcg tttcatatcc cagttgatgt cacgaccggt agaagccagc
     1141 gccgccaggg tgaagcgcag ggcatcggtg ccgtgcggct caatgccgtt cgggaactgt
     1201 ttctcggtgc gcttacggat tttgtccgcc agctgcggct gcatcatatt gccggtacgt
     1261 ttctccagca gttccggcag cgaaataccg tcaaccatat ccagcgggtc aataacgtta
     1321 cccttggatt tggacatctt ctggccttcg tcgtcacgaa tcaggccggt catgtaaacg
     1381 gtgtggaacg gcacctgcgg tttgccattt tcatctttga tgaagtgcat ggtcatcatg
     1441 atcatgcggg caatccagaa gaaaatgatg tcgaaaccag ataccatcac gctggttggg
     1501 tggaactggc gcagtgcgtc ggtgttttcc ggccagccta gggtagagaa ggtccacagc
     1561 gcagaagaga accaggtgtc gagaacgtct tcgtcctgac gcagggcaac gtcggcaccg
     1621 aggttatttt ccttgcgcac ttcttcttcg ttgcggccaa cataaacgtt acctgcttcg
     1681 tcgtaccatg cagggatacg gtgaccccac cacagctgac gagagataca ccagtcctga
     1741 atatcgcgca tccaggagaa gtacatgttt tcgtactgct tcggtacgaa ctgaatgtcg
     1801 ccgttctcaa ccgcctcaac cgccggtttc gccagtacat cggcacgcac gtaccactgg
     1861 tcggtgagca tcggttcgat aactacgccg ccacggtcgc cgtaaggcac ggtcaggtcg
     1921 tgcggtttaa tttcttccag caggccgagc gcgtcaaccg cagcaacgac tgctttacgt
     1981 gcagcaaaac gctccagttt ctggaactct gccgggattt cgctggaata aacgtcagat
     2041 tcgttacctt tggtatcgaa cacctgggcg ctttcacgga tatcgccgtc aaaggtcagg
     2101 atgttgatca tcggcagcgc gtgacgttta cccacttcat agtcgttaaa gtcgtgcgcc
     2161 ggagtgattt tcacgcagcc ggtgcctttt tccatgtcgg cgtgttcgtc gccaacgatc
     2221 ggaatacgac ggttaaccag cggtagaatg acatatttgc caatcaggtc tttgtaacgc
     2281 ggatcttccg ggttaacggc cacgccggta tcgcccaaca gggtttccgg acgggtagtc
     2341 gcaaccacca gataatcttt accgtctgcg gttttcgcgc cgtcagccag cggatagcgg
     2401 atgtgccaca tcgaaccttt cgattcgcgg ttttccactt ccaggtcaga gatagcggtg
     2461 cgcagtttcg gatcccagtt taccaggcgt ttgccacggt aaatcaggtc ttctttatac
     2521 agactaacga aaacttcttt caccgcattg gacaggcctt cgtccatcgt gaagcgttca
     2581 cgctcccagt cgacggagtt gccgagacgg cgcatctgac gggtaatcgt gccgccggat
     2641 tccgctttcc attcccaaat tttgtcgatg aaagcttcgc ggccgtagtc gtgacgggtt
     2701 ttaccttctt ctgcggcaat cttgcgctca acgaccatct gggtagcgat cccggcgtgg
     2761 tcagtaccga cctgccacag ggtgtttttg ccctgcatgc gctgatagcg gatcatggta
     2821 tccatgatgg tttgctggaa ggcgtgaccc atatgcaaac tgccggtgac gttcggcggc
     2881 gggatcatga tgcagaaact ttcctggctt tcatcgccat taggcttaaa gtagccctgc
     2941 ttttcccagt gctcgtaaag cggctgttcg atatcttgtg ggttatatgt cttttccatt
     3001 atttccaggt tgccgtattc aggttgaaac cagccacgcg gtaggcttta tagcgttcgc
     3061 gcgccagttg tttcagagaa tcttcataag gaacgaagtc taccacttct gtgaaagcgg
     3121 tggcaaaatc tgcaaagctt gttcgcagac taatcaatat atcgcgcggg ctgctgctgc
     3181 gtttttgcgg ccaggcgatc tccaccggcg caccgccgcg cggtccttct cccgctaaat
     3241 tatgcggaac aaagctttct gccggacgcg cccacagggc ttcatccagc cggtaagcct
     3301 gcttttcatc ttcacaggcg atgagcacgc gcttaccgct gcgccaacgt tctgcggcaa
     3361 tttcacacac cagttgctca acggtgctta agccatcgac ggtggtgtca ttgtccagaa
     3421 ggtagaacgt cgcgtttttc atatatgggg cttcttgtgg tggatttaaa tacgcatcag
     3481 gtatacaccg cagcgcctga tgcgacgctg ttgtgtctta tcaggcctac gagttcagtg
     3541 ctgtgtaggt cggataaggc gttcacgccg catccggcaa taacagcctt gcctgacgca
     3601 attactcttc gccgttaaac ccagcgcggt ttaacaggaa ctgtgccagc aacgccaccg
     3661 gacgaccggt ggcgcctttt gctttaccag aacgccaggc ggttccggcg atatccaggt
     3721 gcgcccagtt gtacttacgg gtaaagcgcg acaggaagca acctgcggta atcgccccac
     3781 caggacgacc gccaatgttc gccatatcgg caaaattgga ctccagctgt tcctgatact
     3841 cgtcacccag cggtaagcgc catgcgcggt caccggattg ttcagacgcg gcaatcagtt
     3901 catgggccag cggattatgg ttcgccatca gaccggtaat gtgatgaccc agcgcgatca
     3961 cgcaggcacc ggtcagcgtc gccacgtcaa tcaccgcttc cggctcaaaa cgctcaacgt
     4021 aagttaacac gtcgcacagt accaggcggc cttcagcgtc ggtattcagc acttcaacag
     4081 tttgaccgga catggtggtt aacacatcgc ccggacgata ggctcgtccg ccaggcatgt
     4141 tttcgcagcc tgccaacacg ccgataacgt taatcggcag ttgtagctcc gcgaccatcc
     4201 gcatcacgcc gtaaaccgct gccgcaccgc acatatcgta cttcatctca tccatgcctt
     4261 ctgaaggctt gatcgagata ccgccggagt cgaaggttaa acctttaccc accagcacga
     4321 ttgggcgtgc atcttccgac gcgttgcctt tgtactcaat caccgacatc agcgattcgt
     4381 tttgcgaacc ctgaccgacc gccagatagg aatgcatccc cagctctttc atctgctgtt
     4441 cgccgataac gcgggtgatg acattcttgc tgtagctgtc agccagctgg cgcgcttgtg
     4501 aagcgaggta agcggcgtta cagatattcg gcggcatatt gccgagatct tttgctgctt
     4561 taatcccggc ggcaatcgcc agaccgtgca ggatcgcgcg ctcaccgctg gtcagttcac
     4621 ggcgggtcgg cacgttgaac accattttac gcagcggacg acgcggttcg ctcttgttcg
     4681 ttttcagctg atcgaaactg tagagcgtct cttttgccgt ctcgacagcc tgacgcactt
     4741 tccagtagtt gttacggcct ttaacgtgca gttcagtcag aaagcagacc gcttccattg
     4801 agccagtatc attcagcgta ttaatggttt tctgaataac ctgcttgtac tgacgctcat
     4861 ccagctcacg ttctttgccg caaccaataa ggagaattcg ctcggaaagt acattcggaa
     4921 catggtgcag caacaatgtc tgccccggtt ttccttccag ttcgccccga cgtagcaggg
     4981 cgctgatgta cccatcgctg attttatcga gctgttctgc aatcggagaa aggcgacgtg
     5041 gttcgaagac gcccacgacg atgcaggcac tccgctgttt ctccgggcta ccgcttttta
     5101 cactaaactc catgcactac gctcctgaat cttaaagaca acggcggtgg ctacagatag
     5161 aattgcaagc tttcgtaact catgtccgct gttgcgatga cttcgtgtta atcttaacgt
     5221 tattacggca ttggcacgtc agaacaaaat ctgagaggtg aatccgttga gtataattat
     5281 cttagcgacg atttcgacga ctcaagagaa taaatgacgt ttaagccatg aaacaagcta
     5341 aaatcctgca aaagacgagt ttttacgggc gtatttaaag tgataatcat aagatatctg
     5401 gtgcgggaga cgctcaaaag ccagctggcg atactcttca tcttgctttt gatcttcttc
     5461 tgtcaaaagt tagtgaggat cctcggcgca gcggttgacg gcgatattcc ggcgaatctg
     5521 gtgctctccc ttctcgggtt gggcgtgccg gaaatggcgc agcttatcct gccattaagc
     5581 ctgttcctcg ggctgctgat gacgctgggc aaactgtata ccgaaagtga aattacggta
     5641 atgcatgcct gcggcctgag caaagcggtt ctggtgaaag cggcaatgat ccttgcggta
     5701 ttcactgcaa tcgtcgcggc ggttaacgtg atgtgggcgg gaccgtggtc atcgcgtcat
     5761 caggatgaag tgttagcaga agcgaaagcg aaccctggca tggcggcgct ggcgcaaggg
     5821 caattccagc aagcgactaa tggcagctcg gtgctgttca tcgaaagcgt tgacggcagc
     5881 gatttcaaag atgtgttcct cgcgcaaatt cgaccaaaag gtaatgcacg tccttctgtg
     5941 gtggtggccg attccggaca tttaacccag ctgcgcgacg gctcccaggt cgtcactctc
     6001 aaccagggaa cgcgcttcga aggcactgca ctgttacgtg atttccgcat tacggacttc
     6061 caggattatc aggcgatcat tggtcaccag gcggtagcgc tcgacccgaa cgataccgac
     6121 cagatggaca tgcgtacatt gtggaacact gacaccgatc gtgcccgcgc agaactgaac
     6181 tggcgtatca cgttggtatt caccgtgttt atgatggcac ttatggtcgt accgctgagc
     6241 gtggttaacc cgcgtcaggg acgcgtacta tcgatgctgc cagccatgct gctgtatcta
     6301 cttttcttcc tgatccagac ctccctgaaa tcgaacggcg gtaaaggtaa gctggacccg
     6361 acgctgtgga tgtggaccgt taacctgatt tatctggctt tggcgattgt tctcaacctt
     6421 tgggacaccg tgccggtccg ccgcctgcgc gccagttttt cgcgtaaagg agcggtgtga
     6481 tgcaaccttt tggcgtactt gaccgctata tcggtaaaac tattttcacc accatcatga
     6541 tgacgctgtt catgctggtg tcgctgtcgg gcattatcaa atttgtcgat cagctgaaaa
     6601 aagccgggca ggggagttac gacgcgttag gcgcaggaat gtataccttg ctgagcgtgc
     6661 cgaaagatgt gcaaacattc ttcccgatgg cggctctgct tggggcgttg cttggtcttg
     6721 ggatgctggc gcagcgcagc gaactggtgg tgatgcaggc ttctggtttc acccgtatgc
     6781 aggttgcgct gtcggtgatg aaaaccgcca ttccgctggt cttgctgacg atggcgattg
     6841 gtgaatgggt cgcgccgcag ggcgagcaga tggcgcgtaa ctaccgtgcg caggcgatgt
     6901 acggcggctc gttgctctct acccagcaag gcttatgggc gaaagatggc aacaacttcg
     6961 tttacattga gcgggttaaa ggtgacgaag agttaggtgg catcagtatt tatgccttta
     7021 acgagaatcg tcgtctgcaa tccgtacgct atgccgctac tgcgaagttt gacccggaac
     7081 ataaagtctg gcgtctgtcg caggttgatg aatctgatct gaccaatccg aaacagatca
     7141 ccggttcgca gacggtgagc ggcacctgga aaaccaacct cacgccggac aaactgggcg
     7201 tggtggcgct ggacccggat gcactctcaa tcagcggttt gcacaactac gtgaagtatc
     7261 tgaagtcgag cggtcaggat gccggacgtt atcagctcaa tatgtggagc aaaatcttcc
     7321 agccgctatc cgtggcggtg atgatgctga tggcgctgtc gttcatcttt ggcccactgc
     7381 gtagcgtacc gatgggcgtg cgtgtggtca ccggtatcag cttcggtttt gtcttctacg
     7441 tactggacca gatcttcggc ccgctgacgt tggtttatgg catcccgccg atcatcggcg
     7501 cactgttgcc aagcgccagc ttcttcttaa tcagcctgtg gctgttaatg agaaaatcgt
     7561 aaccgaatga acgaataaag ggagcgaaag ctccctttat tattgttagc aaagtgtgct
     7621 tcgttcatgc ctgaaaaatc atgaaaatcc aatatattgc agtggtgtag gcatgataag
     7681 acgcgccagc gtcgcatcag gcaaagacaa atctcgcctg acttaccttc ttcttccccc
     7741 cagcaaactc cccaacatcc cacgtacaat ctgattcgtc acctggcggg cggcgctttt
     7801 cgccattgtt tgcaccacac catctttctt cccgccgcgt ggtccggtag tgccaaacaa
     7861 aatatccttc aatccaccaa gaataccgtc atccaccgcc accgctttcc ctttcgcggg
     7921 aggattattt tgctgcttgg tactggcctg aaagcctttt tgcaacatct cataggcgga
     7981 ttcccggtcc acgtcatcct cgtacttgcc atacaccggg gagtggttaa tcaagccatt
     8041 gcgctcatct tccgtcaccg gtcccatccg cgaacaaggc gcaatcacca tcgcccgttc
     8101 caccacagaa ggacttcctt ttgcatcgag aaacgagatt aacgcctcgc cggtccccag
     8161 ttcctggatt gccttttcgg tatcaaacgc cggattggcc cgcatggttt gcgccgcagc
     8221 ttttaccgct ttctgatctt tgggcgtaaa agcccgcaaa gcgtgttgaa cgcgattacc
     8281 gagctgcccg agcacattat ccggaatatc agacgggttt tgcgaaacga accagacgcc
     8341 cacgcctttt gagcgaataa gccttattac ctgctcaatc ttatccagca gtacctgcgg
     8401 tgcatcgtta aacagcagat gtgcctcgtc gaagaaaaac accagttttg gtttctccag
     8461 atcgcccgct tccggcaatt gttcatacaa ctctgaaagc atccacagca ggctggcggc
     8521 gtacagtttc ggcatctgat aaagcttctc ggcgctgagg atattgataa cgcctttacc
     8581 gttggtatcg gtgcgcatcc agtctttgat atccagcatc ggctcgccaa agaaatgcgc
     8641 cgcaccttgc ttttccagcg acagtaaacc gcgctggatg gcaccaaccg atgcgctact
     8701 gatattaccg tactgattct ggaaagattt ggcgttatcg ccgatgtact gggtaatcgc
     8761 ccgcagatct ttaaagtcga gcagcaacaa tccctgatcg tcagcaatac ggaagatgat
     8821 attcagcacg ccagattgca catcgttgag attcaacagc cgcgccagca acagcggccc
     8881 caggtctgaa accgtcgccc gcaccggatg gcctttctcg ccaaagatat cccacaccac
     8941 caccggatta gcatgcggtt gccagtcatt gacgccgata tttttaagcc ttgtgagcag
     9001 tttttccgac gccgttcctg cctgcgcgat accggtcaga tcgcctttca catcagccat
     9061 aaacaccggc acgccgattt ccgacaatga ctctgccagt ttttgcagcg taacggtttt
     9121 acccgtcccc gttgcgccag taatcagccc gtgacggtta gccattcccg gcagtaaaaa
     9181 aagttctgtg tccggcgtgc gggcaattaa taggggttcg ctcatgagat ttcctccagt
     9241 ttcctgcctg gagtataggc aacggctgcg cactcttcac cgctaaattc agcagattgc
     9301 gagacgcctt ccaaagagat ttaagtgccg ggcgacaggc acccggcaaa gggatcagaa
     9361 gtcggctttc agaaccacgc ggtaacgcgc cttaccgtcg cgcacatgct tgatggcatc
     9421 gttaattttc gacatcggga acagttcggt ggtcggcgca accttgctac gggcggcaaa
     9481 gcgcatcagc tttcgcagct cataaggcgt gccggtagca gaaccagaga tgctgcggtc
     9541 gcccgcaatt aatgtaaagg ccggaacaga cagcggcgtg agaaccgcac cgaccgtatg
     9601 gaaattaccg ccataggtca gcgcctcaaa ataaggctgc cagtcgaggc tgacgttcac
     9661 agtattgata atgagatcaa actgccccga cagggctttc agtgcctgcg gatcgcggct
     9721 attcaccact ttatcggcac ccatcgccag tacttcctgc tctttcgccg gattagaact
     9781 aaaggccgtc acctcgcatc ccattgcgtg cagaagtttt atagcgatat gccccagccc
     9841 gccaatacca attaccccaa cgcggctggt agcagtgata tggtgcatca acagtggttt
     9901 aaagaccgtg ataccgccgc acaacagcgg cccggcggac tcgatatcaa tattttcagg
     9961 cagtggaatc acccattgcc agtccgcacg caacttctcg gcaaagccac cgcgattcat
    10021 aatcgtcggc accgcacctt gctcgcagtt gatctgatta ccgctaatac aggtgtcgca
    10081 gtgaccacag ctacgcgccg tccagccaat cccgacacgc tgaccgacct gcaaaccttt
    10141 atcctgcgcg gcgctcccga gtgccaccac gcgcccaatc acctcatgcc cggcaaccag
    10201 cggatattgt gaaaatcccc attcattgtc gatcatcgat agatcggaat ggcagatccc
    10261 gcaataatcc acctgcactt caacatcttg tggcctcagc tcaccgggat cgtactcata
    10321 aacttccagt tcgccgcccg cttcttttgc ggcatagctt tttatcatcg acattttttg
    10381 gtccttctct ggtgttgttt gggaagtgta gagcatggca gggcggggtg cctggagtgt
    10441 gacaaaggtt acacatcgct gtgtgcaatg ctgaaaattt cagcacttag cgaggtgcga
    10501 gcaagctggc gcttgcatgg tggcgtgcga caggtataat ccacaacgtt ttccgcatac
    10561 ctcttcagtg ccgaagtggc gaaatcggta gacgcagttg attcaaaatc aaccgtagaa
    10621 atacgtgccg gttcgagtcc ggccttcggc accattctta cttccaagac catccgagaa
    10681 agtccaaata tccctttaaa atcaatgctt gcagcgattt tttcgtcctg cgtcttccga
    10741 ggttgtccgt tgaaatccgg atatcattgg gggcataatt gggggcatct taacttcgat
    10801 tagaaatgtg cccccaaatg aagctcaacg ccagacaggt cgagaccgca aagccaaaag
    10861 acaaaaccta caaaatggcc gatggtggtg gtttgtatct tgaggtttcg gccaaaggtt
    10921 ccaaatactg gcgcatgaaa tacagacgtc cctctgacaa aaaagaggat cgtctcgcgt
    10981 ttggtgtttg gcctactgtg acgcttgctc aggcaagagc aaagcgcgat caagctaaaa
    11041 agctgttagc gcagggcatt gatccaaaag ccgaacagaa agaagctcag gccgagaatt
    11101 cgggggcata ttctttcgaa actatcgctc gtgaatggca tgctagtaat aagcgctgga
    11161 gtgaagatca ccgatcacgc gttctacgtt atcttgagct ttatatcttt cctcatattg
    11221 gttcatctga catccgtcag ctcaaaacta gccacctatt agccccgatt aaaaaagttg
    11281 atgccagtgg taagcatgac gttgcacagc gccttcagca gcgtgtcacg gctattatgc
    11341 gttatgcagt acagaacgat tacatcgact caaatccagc cagtgatatg gccggtgcgc
    11401 tatcgacaac caaagcgcga cattaccccg ctttgccctc cagccggttc cctgagtttc
    11461 ttgctcgtct tgctgcatat cgtggccgtg taatgacgcg gatcgcggtt gagctttcct
    11521 tactaacttt tgtgcgttcc agtgagttac gtttcgcgcg ttgggatgag ttcgacttcg
    11581 ataaatctct ctggcgagta cctgcaaaac gagaagaaat taaaggtgtg cgttactcgt
    11641 accgtggcat gaagatgaaa gaggagcata tcgttcagct tagtcggcag gcgatgattt
    11701 tgctaaacca gttaaagcag attagtggag ataaagagct gctttttccg ggtgatcatg
    11761 acgcaactaa ggttatgagc gaaaacacgg taaatagcgc attgcgtgcg atgggctatg
    11821 acacgaaaac cgaagtttgc gggcacgggt ttagaacgat ggcccgtggt gcgttgggcg
    11881 agtccggatt atggagtgaa gatgctatag aacgacagtt gagccattca gagcgtaata
    11941 atgtacgtgc tgcttatatt cacacctctg aacatttgga tgaacgtcga ttgatggttc
    12001 aatggtgggc tgattatctc caagcaaata agggcaaaac aataacacct tatgattttg
    12061 ctaaaataaa caaaagtcga tagtgaaaat aaacgcctat aatatatagg cgtttatttc
    12121 tgtattaaac actttgatca taatcttcca gcagcaactt tttttcgtac ttcatctttc
    12181 caattgccac tatggattat tctatcgaaa ttacttcctg ctcttcctga taataagtca
    12241 attcgagata gcttgctgat atcatcccag ttatcataga ggatatatat agcattacca
    12301 tattctatgt tttcaaatac aaccaaatcg tcctccagca tagctccaat ataattactg
    12361 aacgtgctgt ttcctgtgac gattttttta ggttcaagac tcatgaagaa gtcatatcta
    12421 ttttcagcga catttttctt gtcggaagtg atatttttac ctctaaaaat tcttgctaaa
    12481 gtttcttctt tactacctgg tggaaatatt tcccatgaga ctataagtgt atcagcataa
    12541 gtcgatatgg gtttatcagc atcctcaatt ccacatttac caagattttc ttgaagaaga
    12601 tttaaatcaa ataataattc atcatcaaac ttatttgatt gtttgtctaa aattctagtt
    12661 actctgaagt taaatatgaa atggctgtcc gagggctgtt tgtgattaat ttcaatggct
    12721 atgagtgttg gtggtataat gtctctggga tatttctcgt aaggtaatct aacagtatgt
    12781 gtaccattag agctatcacc ccagttaggt gactcaattt cagtgtaatg agtttctttt
    12841 gggaggtctt ttctgacaat ttcttcacca aaaacatttt taagtgaata tttaccctgc
    12901 aatggatctg gtatgaccga cgtctcataa gcattaacac cattatcaaa atatattcca
    12961 aggtgctcta atgctccact ttttaattgg ttttccgtaa atgcaataga agtgccagca
    13021 acaactgtat taccaagcct tcttaacttg ctatgaatat ttttaggtat aacacgtata
    13081 ttctttttca tttttccctc atacttgatc atcaatgtct ctttgctctc gcttgttatg
    13141 taaatcattg ataaacataa catttgcaag tattacgcta attactgcct caatctgcat
    13201 gtacgaaata gcaataatta atgctaaaga tgtgtttttt attacatctt ggctctctaa
    13261 ataaagatga cttttgaaaa acactagttt aactaagtat acaacaagta aagtaatcat
    13321 aacgaaagat gattttagaa cacttccaac tattgcttct aatcttcttg tatcttccag
    13381 ttgttcggtg aaatcaacgg tttctatagt tttggggttc acaatacgct gcaaagcatt
    13441 tgggtaaaga aacgctaacc aaattcccat aatagtaaaa accattccag aaactgatac
    13501 gagaagagtg caatagttgg caaaatcgtt atacacatag ttaatatcgg atgacgaaat
    13561 aatcatcagc gatataatga atatcgccat gtatatccat gtgtatttgg ctatgcctgc
    13621 tatagctttc aatattcaat cctctccaga tgctttcttt attcttggtt gagattgtaa
    13681 tgcttttaat tcttttaata taggggaaat aaattctttt cttttgtgtg atatttgttc
    13741 ataaatatat gcggcggaat atgttgaata gtcgtcagaa gggacattga gaatatcacg
    13801 cattctatat ttatccaccc aagttatacc cttgtctgta gcaaatccaa cattgtccca
    13861 aagacgtttt tctctatcct cagccgaata cttttcaatg atttcttgaa cttcattcaa
    13921 ggatggtttt gcttctgctt ttatttcaat ttgtcttgtt ttggcatttt tctttccgga
    13981 cacaaatgga acgagagtgc taaaggtctt taaccaatca gctcgtgcat catttggatt
    14041 tataataata gtttccctgc gaactatgtg tgttattttt ggtattagtt tacctaactc
    14101 agtatgagtg gtttctaagc ttctgagctt catatcaaac ctgtacatat atttatataa
    14161 gtcttcgtca tcggtattag atagccttat ataacctttc tcggttttat ttttaaccct
    14221 tttgctatgt ttaactctat tagtaataga tgagtgtaca tagtcttgaa atagttcaga
    14281 atcacaaaca gaatggtcga atttaatgga tactatagtt tcaagttcag gtataaccca
    14341 ataaaagcat ggccgccccc agataacttt tttccctctg tagctattag tatatttaac
    14401 caccgatgag cttccgattt caccgtcttc actagctcct aataaagagc ccgtactatc
    14461 agtatcagat ttccataaca tgactaagaa atcaccagta ttttcatcct tataaatatc
    14521 cttgcaataa cactttgaac tgtttggttt ttcttttgga tcccatggta tcgtgagtgc
    14581 aagagattta gttcccaccc aatcctgtat gaggtcaaat gtttcgctaa gttctaaacc
    14641 ataggtattg tcatcattta ccttgtataa accgcactta ttaaccttga agaaattgag
    14701 atgccctaca ttcatgctct ttccttagct tcttatcttg ggatatgtat catgatcaaa
    14761 aattaagcat aaccccattg acttcgtgcg ggcaatgttt tcaccctaga atttgatcgg
    14821 tttaacgtta aatattcata agttacatga gcatttttca cttcttccca attacagacg
    14881 tcatgagctg aatattaata aaataattat cctcacggca tggcgcgcaa tgctctcccc
    14941 gccacgcccg cccgctttgc agggcggttt taatgcagtt gcacagtcac gctcaggctg
    15001 cgccgggaat ggcgcggtct gcagaaaatg aggcaggaaa acgcatgcaa agccatgcac
    15061 cttatcgatg catggctttt ttcagtaaaa acgggcggat tttcggggaa ttttacacag
    15121 actgacgtga tgccagttgc gcacttttac gcgaaaaaat catgttctgc gcaggggtga
    15181 atttttcacg gctgtcatcc accgaagtcg cgtcaggcct gaatccgatg gtcgttaaaa
    15241 tgtcactatc ctgtgcggaa taattaattt tttccccctt cgcaagccag gacagaaggg
    15301 cttcacgcag ggcatctgtg gcacgctgta tggcacagtt tcgggcaatg gccgtcagct
    15361 cactgtagcc catcagctcc ggtgccagtg ccgccgccag tgctgtgccg tgctgctgca
    15421 taaaatcatt cagccggtcg cggatgctga tgtgctgaac ggcttcatgc gaacgaatat
    15481 aacgaccggc agcctgattc acctgccatt ttctgacttc gataatattg cgtaattcgt
    15541 ccaggcgact gacgtttctg ccttccccgg acagaagccg cagatattcc tgttcggccg
    15601 cagccagctc atttttgcgt tgcagccatg ctgctttgtt attctgacag gtgtcaaagg
    15661 cctgctgtaa ggctgtgctt tccatcgtta tctctttctc atcatgctga agaataaaaa
    15721 tacggtgtgc ggcgacggcc ggtgttaacc ggcagccctc attccagacg cagcgaatat
    15781 gattgtgttt ttaagcgtac tggcggcagt tcctgttttt catgcaggct ttctgccagt
    15841 tcgtccggcg tgaccgggcg gacaataaaa cggttgatgg tctgaagcgt tttaaacacc
    15901 agaccacagc ccggatccgt gcacacataa aaacgctcgg tgacttcctg agacagacgc
    15961 cgcgatgttc ttgacagtgc aaggccttta catctccgac aacaatatcc ggtaacaagc
    16021 attcttttcg ggcgtttcat actgccggag gctgacgtca gtgaatcgcg gtatctctgt
    16081 ttgcctgaaa tgtattccat tcctgaatct ttacagtcag agaaaaagct ttcactcgct
    16141 tcaaatgtcg cagagcaata aatattccgg cactgtgcaa tcattatctt ggtgccatcg
    16201 tccatgaaat gtgcgcgacg ggtgtgagca acatgtccac acgacgggca gtaaatcatg
    16261 acagcagtcc tctggcctta agctctgctc cctgctggtc tattttgtcc tgccacacct
    16321 tgcgctgtgc cggtgtgcct gccacctcat aatccatgtg cgggagtgtt gccgctgaca
    16381 gtccggtcag ccggagaacc ggctcgccgg tgaggctgat ttgcatctgt ttaattttct
    16441 gttccagcga tgatttcacc tgctgcatga cagccttttc cggtgcgacg tagccctgat
    16501 ggccggtggt gttggcgagc ggattttcct gtaccaggat actcagatgc attgcccgga
    16561 caagcgcctc acaggtttca ttcagggcgt gttccagctc atgctctgca tacagactca
    16621 gcaggtgatg atgtgccttc cggtaggcgg tggccgtgct gtcacacgcc cctttcaggc
    16681 gttcacgttc aaaattcagc accacggcca gattgtcata ttcctgtacc agctcccggc
    16741 gtgccacgcg ctcaatgtgg cgctgtttca gctcgtcgct caggacacca ccggctgcac
    16801 gaaaggccgt gcgccagtcg tcagcgtcat ttccgtcggc ctgcgccagc gcattttttt
    16861 cctgctctgc ccgttcaatg gccgtgacgg tctcatccat caggcgggcg ttctcaaggt
    16921 gggcggctct ggccttttcc agttgtgcca gtgcgggttg cagatattca gggatggtgt
    16981 tgtcagacat tttccggctc ctcgtcactt caggttaaga aaattgtgac gtacaccgga
    17041 caacaacacg acgcattgca gatgtgccag ccctgacaca ggagactcat cctcagaccg
    17101 gcaagccagg aaaaggtcgc aggaaaaacc ggcttactgt ttgttttttt atattttact
    17161 gttcacctct gttcaccata agaaaaaaga taagtaatac agtaagttaa agggtgaaca
    17221 atcgcagtaa tgactgttca ccgtctgttc accactgttc acccgctcat agactttttg
    17281 tgctgtttat tactttttat ttttattaat ccactgaaat aaataagaaa aaacaatttg
    17341 tatttcacta taattttttc cagctccttc cagagcgttt taaagccatc tggcgctgat
    17401 gtataaaaaa tacacagcca ttgtaagact gccggaacaa atcccccctg ttgcatctgc
    17461 caaaaatatt cacaaaataa agcgctaccc gaagccggac ggacttatcc ggtgctgtat
    17521 ggacattaac gaggtagccc gatgcaagct gttttttctt ccccgtctcc cgcccctgtg
    17581 acgccactga tgccgctgcc ggacatcacg caggagcgtt ttttacgtct gccggaagtg
    17641 atgcacctgt gcggcctgtc acgctcgacc atctatgaac tcatccgtaa gggggaattt
    17701 ccgccgcagg tgagtcttgg cggtaaaaat gtggcctggc tgcactctga agtcaccgca
    17761 tggatggccg gacgcattgc cggacgcaaa cgggggtacg acgcatgatg atgcccgctc
    17821 tgcaaaaact ccctttttct ggcttgcctt tttccggcat ttgcggatat agtttttccg
    17881 ctgccgcaaa atcggcagcc gggcgtgaga acccgagcaa ttcacaggcg acatatgacg
    17941 cgccacgcgt ctttttttgt gtcgcaatca atgccacaga gcgccagatt atggtgtggt
    18001 gtgtggtcag tcgtgcagat ggcattccgt gcatcctgcc cgcgtccgct cattatgccg
    18061 cagagtcaat ggtagctcag gcggggcagc ctccgggctg gccggtatcc tgtgaagccg
    18121 gtattctcac ccccgtctgg gctatcgcca tcgagcgtga gaactccggc gatagcgtta
    18181 tttgctattc acaggaggct gccatcatgg ctacaaccct taccccctca caccctgaat
    18241 ttgtctttgt gtttgcggct gtccgtcgcg cagaccgtca tccccgtatc tgtatgcttc
    18301 gcaccgtggc cggtgatgaa cgcagtgccc gccgttccct tgtccgtgac tatgtgctct
    18361 cccttgctgc ccgtctgccg gtggtggagg tgtcccgtgc gtaataaaaa agcccctcag
    18421 accgtctcag cacgtcatga cgcccgtgaa cacctcagca ttgaggctta ccataagctc
    18481 aaccgcgcca gcgccgtatc ccagtttgtt gggggtgatt tgattcaccg tgaactctcc
    18541 ggcctgcatc agctctacat tccgcatatt ttcagctacc tgaatgaaga tattgatttt
    18601 gtgctgaatg agctgaaagc caaaggcctg tgccgcgatt ttctcgccca gcagaaagac
    18661 cggggagaca ggacgcatgt ttgattttcc ccagcccggt gagatttacc gttctgccgg
    18721 ttttcccgat gtggccgtgg tcggcattct ggaagacggt attccgtggg aaatgccgta
    18781 ccgctgcccg gacattgtct ggaacccgta ccgccgtaaa ttcagtatcc ttgtgcgtat
    18841 cctcgccgac gggcgcacca cagacatccc gctggggcgt tttctgcggg aatttacctg
    18901 tgaccgtcct gacctgttta aacgcagccc cgtaaaccgg catgcggtac tgaaagaaat
    18961 ggccggagac ccggaattac agaaatggcg ggagaaatat caggatattt acccgcagga
    19021 cactgttccg gtcagccggg cagcaccggt agcgcgggaa tggcgggaaa ttccccgcac
    19081 ggagcctgac ccggaaatca ccccggataa cagttaccgc aattatctgt aattaaaaaa
    19141 cgacacccga aaaattaaat gtgcgtattc gcgcagggat acgcacgtct tcaggagacg
    19201 cagatatgcc ttatcagtta atgcaaccgg cacggaatgc agtcatctgt cacagggagg
    19261 aaagcaaatg aaaacaccct actaccgccc gtcttacgcg cagcccttta ccgtcgtgct
    19321 gtcgcctgtg cctggctgac cgtgtgcgaa cgtcagcacc gctacccgca tctcaccctt
    19381 gagtcactgg aggcggccat cgccgctgag ctggaaggct tttatctgcg ccagcacggt
    19441 gaggaaaaag ggcgtcagat agcctgtgcc ctgctggaag atttaatgga atccggcccc
    19501 ctgaaggccg caccgtcgct gtcctttctc gggctggttg tgatggatga actctgtgcc
    19561 cgtcacataa aagcgccggt actgcactga aggagaacaa caccatgaaa atgaacgtaa
    19621 ccgccaccgt cagccatgcg ctcggccact ggccgcgtat tctcccggcg ctggggattc
    19681 aggtgctgaa gaaccatcat cagccctgtc cggtctgtgg cgggagtgac cgcttccgtt
    19741 ttgatgacag ggaggggcgc ggcacctggt actgcaatca gtgtggtgcc ggtgacggcc
    19801 tgaaactggt tgaaaaggtg tttggtgtct ccccgtccga cgtggccgca aaggtggctg
    19861 ccgtgaccgg cagtctgccc ccggctgacc cggcagtgac ggccgccgcc gttgctgaaa
    19921 cagacgctgc ccggaagaac gccgccgcac tggcacaaac cctgatggca aaaacccgtc
    19981 ccggaaccgg taacgcctac ctgacccgca agggctttcc cggccgggaa tgccggatgc
    20041 tgaccggcac acacagagcc ggtggcgtga gctggcgcgc cggtgacctt gtggtgccac
    20101 tgtatgacga cagcggcgaa ctggttaacc ttcagttaat cagtgctgac ggccgtaagc
    20161 gcaccctgaa aggcggacag gtcaggggca cctgtcacac ccttgaagga cagaatcagg
    20221 ccggaaaacg tctgtggata gcggagggat acgcgaccgc acttaccgta catcacctga
    20281 ccggtgaaac ggtgatggtg gcgctttctt ccgtgaacct cctttctctg gccagccttg
    20341 cccggcagaa gcatccggcc tgtcagattg tccttgccgc agaccgtgac ctcagcggtg
    20401 acggccagaa aaaagccgcc gcagccgcag atgcgtgtga aggtgttgtt gccctgccgc
    20461 cggtcttcgg tgactggaat gatgccttca cgcagtacgg cggggaggtc acccgtaagg
    20521 ccatttacga tgccatccgg ccaccggctg aaagcccgtt cgacaccatg agcgaagcag
    20581 agttttccgc catgagtacc agcgaaaagg ccatgcgtat ctatgagcat tacggcgagg
    20641 cgctcgcggt cgatgccaac ggccagcttc tgtcccgcta tgaaaatggt gtctggaagg
    20701 tgctgccgcc actggacttt gcccgggatg tggccgggct gtttcagcgt ctgcgcgcgc
    20761 cgttctcctc cgggaaggtg gcctccgtgg tggacaccct gaagctgatt attccgcagc
    20821 aggaagcccc ctcccgccgc ctgattggct ttcgtaacgg tgtgctcgac acacagaacg
    20881 gcacgttcca cccgcacagt ccgtcacact ggatgcgtac cctgtgcgat gtggatttca
    20941 ccccaccggt ggaaggggaa acgctggaaa cccacgcccc cgcgttctgg cgctggcttg
    21001 accgtgccgc cggtggccgt gcggaaaaac gcgacgtgat tctggccgca ctgtttatgg
    21061 tgctggcaaa ccgctacgac tggcagctct ttctggaggt gaccggtccc ggcggcagcg
    21121 gcaaaagtat catggccgaa atcgccaccc tgctggccgg ggaggataac gccacgtcgg
    21181 ccaccattga gacgctggaa tccccgcgtg aacgtgccgc gttaactggc ttctcactga
    21241 tacgcctgcc ggaccaggaa aaatggagcg gcgacggtgc cggactcaag gccatcaccg
    21301 gcggcgatgc ggtgtccgtg gacccgaaat accgggatgc gtactccacg catatcccgg
    21361 cggtgattct ggccgtgaac aataacccga tgcgcttcac cgaccgcagc ggcggcgtgt
    21421 cacgccggcg ggtgattatt cacttcccgg aacagatagc cccgcaggag cgcgacccgc
    21481 agcttaagga caaaatcacc cgcgagctgg cggtcatcgt gcgtcacctg atgcagaagt
    21541 tcagcgaccc gatgctcgcc cggtcactgc ttcagtccca gcagaactca gacgaggcgc
    21601 tgaacatcaa acgggatgcc gacccgacgt ttgattttat cggctatctg gaaaccctgc
    21661 cgcagaccag cggcatgtat atggggaacg ccagtatcat cccgcgtaat taccgtaaat
    21721 acctctatca cgcctatctg gcctacatgg aggcaaacgg ctaccggaat gtactcagtc
    21781 tgaaaatgtt cgggctgggg ctgccggtga tgctgaagga atacggactg aattacgaga
    21841 agcgccatac caaacagggg atacagacca acctgacact gaaagaggaa agctacggcg
    21901 actggctgcc aaaatgtgac gaccctgcaa caacctgacc cacctgaccg gcatctgccg
    21961 gtcttttttt atcccgacct cccccgaagg tgaacaatcc actgttcacc cttcaccgta
    22021 tattcacccg ttatcacact gaaattaaaa gagaaaaatg aaaggtgaac agtgtgaaca
    22081 gttctttcga aaaaaaattt tttatgtgga taaaagatgc actggtttgg atccgggcta
    22141 cggatccaaa cttactgatt aaaaattatt taactttccg atttattgat gggctgaaaa
    22201 tagattggtc agaaaaacag tgggggcaca aaagggggca tatttcattg ttgtgtttta
    22261 ttatttgttt tttatcatta acttattttg atatttgagt ccggccttcg caccaaaagt
    22321 atgtaaatag acctcaactg aggtcttttt ttatgcctga aatccgcgct acacaaggct
    22381 tttccagctt ttcccctcaa ctgaagtcaa cctgactcaa cccacatcaa caggcttgtg
    22441 agtatactaa tgagtatatt tgccgattcg atattgcttg tatactcacg agataccaat
    22501 ggaaggaaga ccattatggc cctaacggat actaaagttc gctctgcgaa acctgaggag
    22561 aaagagtatt cacttgttga cggcgacggc atgtccttac ttgtaaaacc tggcggttca
    22621 aagtattggc gatttcgttt ccgttttggc ggtaaacaac atttgatggc gttcggcgtt
    22681 tacccggatg tttcgctggc ggatgcgcgg aagaaaaggg aagaggccag aaagctggta
    22741 gctgctggta tcgatcctcg tgagcataag cgtgctgtga aagaagagca ggcgaaagag
    22801 attattactt tcgagaaggt tgccagagag tggctcgtaa ccaaccaaaa atggtcggaa
    22861 gagcatgcta atcgtgtaaa aaagagcctg gaggacaata tcttcccggc aattggttcc
    22921 cacaatattg ctgaactggg cacccgcgat ctgttaattc ctattaaagc cgtggagaaa
    22981 tccggacgtc ttgaagtggc ttcccgcctt caacagcgca ccacggccat catgcgttat
    23041 gcagtgcaaa gcgggttaat tgattacaat ccggcgcaag agatggcggg ggcggtagct
    23101 tccagtaatc gacagcatcg tccggcgctg gagttaaagc gtatcccgga attactccag
    23161 aaaatcgatg aatacaccgg caggccacta acccgctggg caaccgaact taccctgctt
    23221 atttttattc gatccagtga gctgcgtttt gctcgctggt cagaaatcga ttttgaaacg
    23281 tcaatgtgga ccatcccgcc tgagcgagaa cctattcccg gtgtgaaaca ttctcagcga
    23341 ggagcaaaga tgcgtacacc tcatctggtg ccgctttcaa agcaggcgct agcgatcctg
    23401 aagcagataa aacagttctg tggcgaacat gagctgattt ttattggcga tcacgatccg
    23461 agtaagccca tgagtgaaaa cacggtgaac agtgcattgc gggtgatggg ctatgacacc
    23521 aaggttgagg tttgcggcca cggcttccgc actatggcct gtagctcatt gattgagtca
    23581 gggttatggt cgaaggatgc ggtggaaagg cagatgagcc atatggaacg caattcggtg
    23641 agggcggcgt atatccataa agcggaacat ctggatgagc gcaagctgat gttgcagtgg
    23701 tgggctgatt ttctggatgc gaaccgggag aaggggatta cgccgtttga ctatgcgaag
    23761 atcaatcggg gtaatggcga gtgagttatc taaaagcgca cattcgcctc taaattgaaa
    23821 gctgctgact tattcaaacg aggaattggt cagcagtgcc aaatagccga gttttcacta
    23881 aggtaagcct ttttattgaa cctaatcggc agtaatgctc cacctaagcc taaatttaaa
    23941 gtcatgatta cttataaaag gggagtggac ccttgattga gccctacatc gtaacaaagg
    24001 tacagcaatt cggctacatc aggagtgata gtcaattagt taatctaata attaattgaa
    24061 tctaaataat taaatttctt acgtaaaatt gaccaattta ctggtggtgt aagccgcaat
    24121 atcttaatgt aatcagtata aaggggcttg atttctttta aagatagtat ccgtatgaat
    24181 tttaagaaaa aactcgaaga gcattttaag cagttcgaag catccccagt gttatttgtc
    24241 ggctccggtg tgtctcgtcg ttatcttggt gtgccatgtt ggcaagacct tttgaagcat
    24301 tttgctgagg caattgggga aaatcatata aaattaaaaa caaaatctaa tggtgactta
    24361 cccgaatatg cacaattatt ggtgtctgca tatgctgaga aatggtggga tactgaagaa
    24421 ggacaattag cattaagtga aaaagaacag gagaaaactt tcattaacga acagtctcct
    24481 ttgaagttgt caatatctaa atatattgag aatgcacata aaaatattat agataatgat
    24541 gagttaaaac atga
//
LOCUS       DAJTSL010000050        24539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTSL010000050 DAJTSL010000000
VERSION     DAJTSL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.050"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(396..1277)
                     /gene="yddG"
                     /locus_tag="OO433_001808"
     CDS             complement(396..1277)
                     /gene="yddG"
                     /locus_tag="OO433_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6050313.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEAGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTQQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1509..4556
                     /gene="fdnG"
                     /locus_tag="OO433_001809"
     CDS             1509..4556
                     /gene="fdnG"
                     /locus_tag="OO433_001809"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2094..2096,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6050314.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIAVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGERVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4569..5453
                     /gene="fdnH"
                     /locus_tag="OO433_001810"
     CDS             4569..5453
                     /gene="fdnH"
                     /locus_tag="OO433_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6050315.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEAPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            5446..6099
                     /gene="fdnI"
                     /locus_tag="OO433_001811"
     CDS             5446..6099
                     /gene="fdnI"
                     /locus_tag="OO433_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6050316.1"
                     /translation="MSKSKMIVRTKFIDRVCHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(6150..6434)
                     /locus_tag="OO433_001812"
     CDS             complement(6150..6434)
                     /locus_tag="OO433_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6050317.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(6580..7590)
                     /gene="adhP"
                     /locus_tag="OO433_001813"
                     /pseudo
     CDS             complement(6580..7590)
                     /gene="adhP"
                     /locus_tag="OO433_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            complement(7724..9421)
                     /gene="maeA"
                     /locus_tag="OO433_001814"
     CDS             complement(7724..9421)
                     /gene="maeA"
                     /locus_tag="OO433_001814"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6050318.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     IDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(9578..9715)
                     /gene="sra"
                     /locus_tag="OO433_001815"
     CDS             complement(9578..9715)
                     /gene="sra"
                     /locus_tag="OO433_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCR6050319.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(9817..10032)
                     /gene="bdm"
                     /locus_tag="OO433_001816"
     CDS             complement(9817..10032)
                     /gene="bdm"
                     /locus_tag="OO433_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCR6050320.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10378..10809
                     /gene="osmC"
                     /locus_tag="OO433_001817"
     CDS             10378..10809
                     /gene="osmC"
                     /locus_tag="OO433_001817"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6050321.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSFMLGEAGFTPTSIDTIADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(10865..11791)
                     /locus_tag="OO433_001818"
     CDS             complement(10865..11791)
                     /locus_tag="OO433_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6050322.1"
                     /translation="MSDTLLTLRGVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALVEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(11784..12770)
                     /locus_tag="OO433_001819"
     CDS             complement(11784..12770)
                     /locus_tag="OO433_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6050323.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIVLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVRADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(12767..13663)
                     /locus_tag="OO433_001820"
     CDS             complement(12767..13663)
                     /locus_tag="OO433_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6050324.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMFFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPRAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(13660..14682)
                     /locus_tag="OO433_001821"
     CDS             complement(13660..14682)
                     /locus_tag="OO433_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6050325.1"
                     /translation="MTFWSILRQRCWGLVLVMAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(14684..16234)
                     /locus_tag="OO433_001822"
     CDS             complement(14684..16234)
                     /locus_tag="OO433_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6050326.1"
                     /translation="MKRSIPFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLA
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDKDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(16248..16829)
                     /gene="ddpX"
                     /locus_tag="OO433_001823"
     CDS             complement(16248..16829)
                     /gene="ddpX"
                     /locus_tag="OO433_001823"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR6050327.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(17087..19486)
                     /gene="dosP"
                     /locus_tag="OO433_001824"
                     /gene_synonym="pdeO"
     CDS             complement(17087..19486)
                     /gene="dosP"
                     /locus_tag="OO433_001824"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR6050328.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTDLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNHDYL
                     LSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(19511..20892)
                     /gene="dosC"
                     /locus_tag="OO433_001825"
                     /pseudo
     CDS             complement(19511..20892)
                     /gene="dosC"
                     /locus_tag="OO433_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            complement(21264..22583)
                     /locus_tag="OO433_001826"
     CDS             complement(21264..22583)
                     /locus_tag="OO433_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6050329.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(22714..24249)
                     /gene="gadC"
                     /locus_tag="OO433_001827"
     CDS             complement(22714..24249)
                     /gene="gadC"
                     /locus_tag="OO433_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6050330.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT         6125 a         6183 c         6189 g         6042 t
ORIGIN      
        1 ttagtaaaat aacgtagagc ggtaaccttg ccataaaggt cgagtttatt accatccttg
       61 ttatagactt cggcagcgtg tgctacgttc gcagctaaca aaccagttac aaccaccgca
      121 actattttta atttcattta acagaatcct tttaattatc gtcaaacgta ttttctaaac
      181 gaattttaaa cggcgtcatt tataaatgac atactgtttt taaaatagaa tggtgattaa
      241 aaacaatgag aaaaatagat gagataatat tatttatcga tatgtgatcg aagtcgaaat
      301 gagatataag gtgaattact ggtatttgaa atttattttt taatattgtc ggaatttatc
      361 tgattaactg ccgggccgta gacccggcaa ttattttaac cacgacgtgt cgccagccag
      421 cagagcaggg aaccgccgca gaccattagc gcgccttgcc agaatgagaa cgacagcggg
      481 gcgctgagca gcacggctgc aagcgctgag gaaagtacgg gcgtaaaata cgagcctacc
      541 gccattatgg tgacattgcc atgcaatata ccgacattcc atgcagcata agcaaatcct
      601 aaggtaaatg ccgcagagat gagtttaatc atgacgggcg tgctaaatac catttctggt
      661 tgttgcgtaa gaaaatagta aacccacaga cttgctcccg ttagcaggac aaaaacggta
      721 attccgttaa atccgcgtgc gtatttattc gttactgtgc aataggctgc ccagataaac
      781 gcaccaataa acgccaggaa ataactcaat gggctggtgg tgatattatt gatgatttca
      841 tcataatgta acccattctc accgcctaac acccagcaga cgccgaccag ggctaataat
      901 aatcctggta caatcagcca gttggttttc tgaccattaa acagaatggc aaagagaatt
      961 gtcaggctgg gccacagata gttcaccata cccgcttcaa tcgcctgatg acgggtcgcc
     1021 gcatacccta aggaaagcgc cagacagatt tcatagctga cgaataacag actcccggcg
     1081 agtaaatagc ctttcggaat ctgccgaata cgcggaaatc caaccgtgaa gattaacagc
     1141 agcccgctta atgaatagat agcagctgcg ccgccgaccg ggccgagccc ctcgctgaca
     1201 ccgcgaatca atcctaccat cgtgctccac aggacgatcg ctattagccc tatgagcgtt
     1261 gctttttgtc gtgtcatgct cgctgttttg tctctcttgc cgttaaaaat taagctgaat
     1321 tttatagcat ttttttaact ggcctgtcag gcagtggtgc gtttttctac cgctattgag
     1381 gtaggtcaat ttgcgcaggc ggattatttt gtggcaaaca cgtgttcttt ttgatttcgc
     1441 gcaaaaagat tcagaatttt actgttagtt tcctcgcgca gtaatatccc tgaaaaaaga
     1501 ggaaagcaat ggacgtcagt cgcagacaat tttttaaaat ctgcgcgggc ggtatggctg
     1561 gaacaacggt agcggcattg ggctttgccc ctaagcaagc actggctcag gcgcgaaact
     1621 acaaattatt acgcgctaaa gagatccgta acacctgcac atactgttcc gtaggttgcg
     1681 ggctattgat gtatagcctg ggtgatggcg cgaaaaacgc cagagaagcg atttatcaca
     1741 ttgaaggtga cccggatcat ccggtaagcc gtggtgcgct gtgcccaaaa ggggccggtt
     1801 tgctggatta cgtcaacagc gaaaaccgtc tgcgctaccc ggaatatcgt gcgccaggtt
     1861 ctgacaaatg gcagcgcatt agctgggaag aagcattctc ccgtattgca aagctgatga
     1921 aagctgaccg tgacgctaac tttattgaaa agaacgagca gggcgtaacg gtaaaccgtt
     1981 ggctttctac cggtatgctg tgtgcctccg gtgccagcaa cgaaaccggg atgctgacac
     2041 agaaatttgc ccgctccctc gggatgctgg cggtagacaa ccaggcgcgc gtctgacacg
     2101 gaccaacggt agcaagtctt gctccaacat ttggtcgcgg tgcgatgacc aaccactggg
     2161 tggatatcaa aaacgctaac gtcgtaatgg taatgggcgg taacgctgct gaagcgcatc
     2221 ccgtcggttt ccgctgggcg atggaagcga aaaacaacaa cgatgcaacc ttgatcgctg
     2281 tcgatcctcg ttttacgcgt accgcttctg tggcggatat ttacgcacct attcgttccg
     2341 gtacggacat tacgttcctg tctggcgttt tgcgctacct gatcgaaaac aacaaaatca
     2401 acgccgaata cgttaaacat tacaccaacg ccagcctgct ggtgcgtgat gattttgctt
     2461 tcgaagacgg cctgttcagc ggctacgacg ctgaaaaacg ccagtatgat aagtcgtcct
     2521 ggaactatca gttcgatgaa aacggctatg caaaacgcga tgaaacactg actcatccgc
     2581 gctgtgtgtg gaacctgctg aaagcgcacg tttcccgcta cacgccggac gtcgttgaaa
     2641 acatctgcgg tacgccaaaa gccgacttcc tgaaagtgtg tgaagtgctg gcctccacca
     2701 gcgcaccgga tcgcacaacc accttcctgt acgcgctggg ctggacgcag cacaccgtgg
     2761 gtgcgcagaa catccgtact atggcgatga tccagttgct gctcggtaac atgggtatgg
     2821 ccggtggcgg cgtgaacgca ttgcgtggtc actccaacat tcaggggctg actgacttag
     2881 gtctgctctc taccagcctg ccaggttatc tgacgcttcc gtcagaaaaa caggttgatt
     2941 tgcagtcgta tctggaagcg aacacgccga aagcgacgct ggctggtcag gtgaactact
     3001 ggagcaacta tccgaagttc ttcgttagcc tgatgaaatc tttctatggc gatgccgcgc
     3061 agaaagagaa caactggggc tatgactggc tgccgaagtg ggaccagacc tacgacgtca
     3121 tcaagtattt caacatgatg gacgaaggca aagtcaccgg ttatttctgc cagggcttta
     3181 acccggttgc gtccttcccg gacaaaaaca aagtggtgag ctgcctgagc aagctgaagt
     3241 acatggtggt tatcgatccg ctggtgactg aaacctctac cttctggcag aaccacggcg
     3301 agtcgaacga tgtcgatccg gcgtctattc agactgaagt attccgtctg ccttcgacct
     3361 gctttgccga agaagatggt tctatcgcta actccggtcg ctggttgcag tggcactgga
     3421 aaggtcagga cgcgccgggc gaagcgcgta acgacggcga aattctggcg ggtatctacc
     3481 atcatctgcg cgagctgtac caggccgaag gtggtaaagg cgtagaaccg ctgatgaaga
     3541 tgagctggaa ctacaagcag ccgcacgaac cgcaatctga cgaagtggct aaagagaaca
     3601 acggctacgc gctggaagat ctctatgacg ctaatggcgt gcttattgcg aagaaaggtc
     3661 agttgctgag tagctttgcg catctgcgtg atgacggtac aaccgcatct tcttgctgga
     3721 tctacaccgg tagctggaca gagcagggca accagatggc taaccgcgat aactccgacc
     3781 cgtccggtct ggggaatacg ctgggatggg cctgggcgtg gccgctcaac cgtcgcgtgc
     3841 tgtacaaccg tgcttcggcg gatatcaacg gtaaaccgtg ggatccgaaa cggatgctga
     3901 tccagtggaa cggcagcaag tggacgggta acgatattcc tgacttcggc aatgccgcac
     3961 cgggtacgcc aaccgggccg tttatcatgc agccggaagg gatgggacgc ctgtttgcca
     4021 tcaacaaaat ggcggaaggt ccgttcccgg aacactacga gccgattgaa acgccgctgg
     4081 gcactaaccc gctgcatccg aacgtggtgt ctaacccggt tgttcgtctg tatgaacaag
     4141 acgcgctgcg gatgggtaaa aaagagcagt tcccgtatgt gggtacgacc tatcgtctga
     4201 ccgagcactt ccacacctgg accaagcacg cattgctcaa cgcaattgct cagccggaac
     4261 agtttgtgga aatcagcgaa acgctggcgg cggcgaaagg cattaataat ggcgagcgtg
     4321 tcactgtctc cagcaagcgt ggctttatcc gcgcggtggc tgtggtaacg cgtcgtctga
     4381 aaccgctgaa tgtaaatggt cagcaggttg aaacggtggg tattccaatc cactggggct
     4441 ttgagggtgt cgcgcgtaaa ggttatatcg ctaacactct gacgccgaat gtcggtgatg
     4501 caaactcgca aacgccggaa tataaagcgt tcttagtcaa catcgagaag gcgtaagggg
     4561 gcgaacagat ggctatggaa acgcaggaca ttatcaaaag gtccgcaact aactccatca
     4621 cgccgccttc tcaggtgcgt gattacaaag cagaagtcgc aaaacttatc gacgtttcca
     4681 cctgtatcgg ctgtaaagcc tgtcaggtgg cgtgttcgga gtggaacgac atccgtgatg
     4741 aagtggggca ctgcgtcggg gtttacgata accccgccga tctgagcgcc aagtcctgga
     4801 cggtgatgcg ctttagcgaa accgaacaga acggcaagct ggagtggctg atccgtaaag
     4861 acggctgtat gcactgtgaa gctcccggct gcctgaaggc gtgcccgtct gctggtgcaa
     4921 tcattcagta cgctaacggg attgtcgatt tccagtcgga aaactgcatc ggctgtggtt
     4981 actgcattgc cgggtgtccg tttaatattc cgcgcctcaa caaagaggat aaccgggtat
     5041 ataaatgcac gctctgcgtc gatcgcgtca gcgtcggcca ggaaccggct tgtgtgaaaa
     5101 cctgtccgac cggggctatc cacttcggca ccaagaagga gatgctggag ctggcggaac
     5161 agcgcgtggc gaaactgaaa gcgcgtggtt acgaacatgc tggcgtctac aacccggaag
     5221 gggtcggtgg tacgcacgtt atgtacgtgc tgcatcacgc cgatcagccg gagctgtatc
     5281 acggtctgcc gaaagatccg aagatcgaca cctcggtaag cctgtggaaa ggcgcgttga
     5341 aaccgctggc agcggctggc tttattgcca cttttgccgg gttgattttc cactacatcg
     5401 gtattggccc gaataaggaa gtggacgatg acgaggagga tcatcatgag taagtcgaaa
     5461 atgattgtgc gcaccaaatt tattgatcgc gtctgtcact ggaccgtggt gatttgcttc
     5521 ttcctggtgg cgctgtccgg gatttcgttc ttcttcccga cgctgcaatg gctgacgcaa
     5581 accttcggta cgccgcagat gggacgcatt ttgcacccgt tcttcggcat tgcgattttc
     5641 gtcgcgctga tgtttatgtt tgtgcgtttt gtgcatcaca acatcccgga taagaaagat
     5701 attccgtggc tattgaacat tgtcgaagta ttaaaaggca atgagcacaa agtggcggat
     5761 gttggtaagt acaacgccgg gcaaaagatg atgttctggt cgatcatgag catgattttc
     5821 gtgctgttgg tgaccggggt gattatctgg cgtccgtact ttgcgcagta cttcccgatg
     5881 caggttgttc gctacagcct gctgatccac gcggctgccg gtatcatcct gatccacgcc
     5941 atcctgatcc atatgtatat ggcattctgg gtgaaaggat cgattaaagg gatgatcgaa
     6001 gggaaggtaa gccgtcgctg ggcgaagaaa caccatccgc gctggtatcg tgaaatcgag
     6061 aaggcagaag cgaaaaaaga gagtgaagaa gggatataat ctcttttgaa ctttaagctg
     6121 aaaatggcgc tttaagaagc gccatttttt tattgcgtaa ccagacggcg cagtcgcgac
     6181 acatccaccg ttttttcagc ctctgccaga ctccaggcgt tttgcagatt cagccacatt
     6241 tgcggcgaac tgccgatcac cacggaaagt ttaattgcca tttctggcgt caaagctgct
     6301 tttccggtca gcaatcggct tgccgttgag ggcgcaattt ccattgctct ggcaaactcg
     6361 cgcaggctga cattaagttc gtccagtgat tcctgaataa tgtcccccgg gcggggatga
     6421 ttggccattt tcattagtgt ctttactctc agtagcgttc cttctctggg aacactatca
     6481 ttattgttcc ttcctacgca acaataatcc atttagccat ttcatgttgg agagtgtgcg
     6541 atgctgcgac ccgaacatgg gagtcgcagc aaagacctct tagtgacgga aatcaatcac
     6601 catacggcca cggattttgc cttcttccat ctcggtaaag atggtgttga tgtccgctaa
     6661 cggacgcagg gcgactttcg gcaccacttt gccttcggcg gcaaactgga aggcttcggt
     6721 tagatcctgg cgcgtgccga ccagcgaacc gaccacttca atgccatcca gcacaagacg
     6781 tgggatatcc aggctcatag actccggcgg cagaccgaca gccacaacac gaccgcctgc
     6841 acggacagca tcaactgccg agttaaacgc agctttagct actgctgtta ccaccgcagc
     6901 gtgagcgcca ccggttttct cctgcacaat ttaggcggcg tcttcggtgc gtgagttaat
     6961 cgctaaatct gcacccattt cggttgccag ttttaactgc tcatcattga catcaatggc
     7021 gatcactttg gcgttaaaga cattcttcgc gtattgcagg gcgaggttac ccagaccgcc
     7081 aagaccgtag atagcaatcc actgaccggg acgaattttt gacagtttaa cggctttgta
     7141 ggtggtgacc cccgcacagg taatgctgct ggccgccgcc gagtccagac catctggcac
     7201 ttttaccgcg taatcggcga ccacgatgca ctcttccgcc atcccgccat caacgctgta
     7261 tccggcattt ttaactgaac ggcagagcgt ttcgttacca ctgttacagt attcgcaatg
     7321 accgcatcct tcgtaaaacc acgccacgct ggcacgatcg cctggtttta atgaggtgac
     7381 acctgggcca acttctgcca ccacaccgat gccttcatgg cccagaatta ccccagtttt
     7441 gtcaccaaaa tcgccattct taacatgaag atcggtatga catacaccac aacactccat
     7501 tttcagcagg gcttcgccat gtttcagtga gcgcagtgtt ttatccgtaa cgtcaacatg
     7561 atgatccttc gtaacaactg cagccttcat agttcctcct tttcggatga tgttctgcat
     7621 agcaggtgag gcaaatgaga tttattcgcc actacccagt atggatgaga tctgaaaaag
     7681 ggagagggaa atagcccggt agccttcact accgggcgca ggcttagatg gaggtacggc
     7741 ggtagtcgcg gtattcggct tgccagaaat tatcgtcaat ggcttgttgc aaagcttcag
     7801 cagacgtttt caccgccacg ccttgctgct gcgccatttt gccaaccgca aacgcaattg
     7861 cgcgggagac tttctgaata tctttcagtt ccggtagtac cagaccttcg ccgttcagca
     7921 ccagcggcga atactgagca agcgtttcac ttgctgacat cagcatctca tcggtgatac
     7981 gtgacgcgcc ggaagcaata acacccagcc cgatgccagg gaaaataaag gcgttgttac
     8041 actgggcgat agggtagatt ttatctttcc ataccactgg attaaacggg ctgccagtgg
     8101 cgaccagcgc gttaccttcg gtccaggcga taatgtcctg cggtgtggct tccacgcgtg
     8161 acgtcgggtt agacagcggc atcacgatcg gacgcggaca gtgtttatgc atctcacgga
     8221 tgatctcttc cgtaaacagc ccggtctgtc ctgagacgcc aatcagaata tctggtttta
     8281 cattgcgcac cacatccagc agtgacagca catcgctgtc ggtatcccag tcactgaggt
     8341 tttcgcgttt ctgcaccagt ttggtctgga aaggcagcag gttcggcatc ttgtccgtca
     8401 gcaggccaaa gcgatcgacc ataaagactt tctgccgcgc cgcttcctcg cttaatcctt
     8461 cgcgctgggt ctgggcgatg atcatttcgg caatgccgca tcccgctgaa cctgcaccaa
     8521 ggaagacgat ttttttctcg cttaactgac cacctgccgc tcggcttgct gcgatcagtg
     8581 tgccgactgt taccgccgca gtgccctgaa tgtcatcgtt aaaagaacaa atttcattgc
     8641 gatagcggtt aagtaacggc atcgcatttt tttgagcaaa gtcttcaaac tgcaacagca
     8701 cgtctggcca gcgttgtttc acagcctgga taaattcatc aacgaattca tagtattcgt
     8761 cgtcaatgat acgcggatta cgccagccca tatacagcgg atcgttaagc agctgttggt
     8821 tgttcgttcc gacatccagc accaccggaa gggtatacgc cgggctgatg ccgccacagg
     8881 cggtatagag cgacagttta ccgatcggaa tgcccatccc gccgatgccc tggtcaccaa
     8941 gccccagaat acgttcaccg tcagtcacca caatcacttt gatattatgg ttcggtacgt
     9001 tttgcagaat atcgtccata ttgtggcggt tctggtaaga gataaacacg ccgcgtgaac
     9061 ggcggtagat ctcagaaaaa cgctcacagg ctgcgccgac ggttggggta taaataacag
     9121 gcatcatctc atcaagatga ttgtttacca gacggtagaa gagggtttcg ttggtgtcct
     9181 ggatgttacg caggtagatg tgtttgtcga tttcggtttt gaatccctga tactggatcc
     9241 atgctcgttc cgcttgttct tcgatggttt cgaccacttc cggcagtaac cccagcaggt
     9301 tgaagttacg gcgttcttcc atgctgaagg cactgccttt attcaacaac ggaaattcca
     9361 gcagtacagg gccagcgtaa gggatataaa gcgaacgctg tttttttgtt tttggttcca
     9421 tgtcactcac tcttttttga atatccatcc ctggcacgct tttatcgtct ttgctttacc
     9481 gccagggcgt cggccccaag tataaagcag ataaaaacaa aaacaccatt gcgcaggcaa
     9541 tggtgtttaa tcgtcattga ggactgatgg ttatgaatta cttttcagcg gggcgttttc
     9601 tgccggttgg gttatttacc acgctggatt tgtcaccttc ggtaactatt ttgcgctggt
     9661 tagaaatttt atggtccagt ccaagaatat gacgtgcctg acggttcgat ttcattttaa
     9721 ctcctcaatc ctgttgctag ttttaaggac aacatcgccg tagcgaagaa acacgtgcta
     9781 aacccctaaa ttaggttgcc gatcaagcat agcaccttaa agcgtagggt gctggccact
     9841 gaccacataa ttgatcgttt gctggtagat atcactgagg atgtcgttat cagaagcttc
     9901 cacccatttg gtcagctcca tgagaatgtc atcttcagtg acaacaccgt gctctgcccg
     9961 aaggccttgc tcaatggcat taaccagagc gggttctgct gttgaatttt ctgcctgata
    10021 ataagtaaac atagtgattc tccgtgtctg tgtatttatg gtgtctgcta cggatcgcag
    10081 atttataaag cacattcagc atggcaaata tttgccgctt tgttgttaag attagtcctg
    10141 gttgatgatt tttatatttt aacctcatga tattgatagg tattattatt gtggtgagtt
    10201 gattaatatt aatgcaatat ctcttgggtt atatattccc ggttatctat tgtggaaatt
    10261 taatttaagt gcagaagtaa tatttctgcc ggatttttat tcggaatatc ctgcttatcc
    10321 tcgtgctgtt tctcacgtag tctataattt cctttttaag cccacatgag agcaacaatg
    10381 acaatccata agaaaggtca ggcacactgg gaaggcgata tcaaacgcgg gaagggaaca
    10441 gtatccaccg agagtggcgt gctgaaccaa cagccgtatg gatttaacac gcgttttgaa
    10501 ggcgaaaaag gaactaaccc tgaagaactg attggcgcag cgcatgccgc atgtttctca
    10561 atggcgcttt cattcatgct gggggaagcg ggatttacgc caacatcgat tgataccatc
    10621 gccgatgtgt cgctggataa agtggatgcc ggttttgcga ttacgaaaat cgcactgaag
    10681 agtgaagttg cggtgccggg tattgatgcc tctacctttg acggcatcat ccagaaagca
    10741 aaagcaggat gcccggtctc tcaggtactg aaagcggaaa ttacgctgga ttaccagttg
    10801 aaatcgtaaa gcattgccgg atgacgtgtc aggcgcgtga atgcctgatg cgttgttagc
    10861 atctctacag cgcccgccag caccggagct cacgtccgtc ctcccttatt gctaatgatt
    10921 gccggacttc acatccgctg gttgcaaggg ggcaacgttc gtagaaaaaa cagccttgcg
    10981 gcaacgtgcg gtttcccggc agatccgttt tacgtaatgc ccattcttcc tccagcggtt
    11041 tatcaatggc ggggagggaa tccagcaata atcgggtgta tggatgtgcc ggagccgtta
    11101 gcacctgttg cgcgtcgccc agttctacaa tctgcccgag atacatcacc gccacccgat
    11161 cgctcatatg acgtatcacc gagacattgt gtgaaatcag cacataggtc agcccgtgat
    11221 tttcctgtag cgttaccagt aaattgagga tctgcgcctg cacggagata tccagcgcag
    11281 aggttggctc atcaagcaca atcacgtcag gctgcgaaga gagtgctctg gcaatggcga
    11341 tacgttgccg ctgcccgccg gagaacgcat gaggcaggcg gtcgagatac tccggacgaa
    11401 tacccacctg cacagccagc tcctctacca acgctcgccg ctgttgttca ctactacgct
    11461 tagctatcca gagcggttct gtgatgatgc gccacaccgg taagcgcgga ttgagcgaag
    11521 agagcgggtc ctgaaacacc atctgcataa tgcgttgtga gccagaacgg atgtactgcc
    11581 cgtggctcgg ttgcagcata cccattaaaa gctgtgcgag ggtgcttttg ccgcagcctg
    11641 actcgccgac gatccctaag gtttcaccac ggcggatctg taaatcaata ccattaatcg
    11701 catgaacatg ttccgtagtt ttaccaagcc agtttttacg ggccgggaaa ttgatatgga
    11761 cgccgcgtaa cgttaataac gtgtcagaca cttatgacct cctgctgcgg ataccaacag
    11821 gcgcagcgct ggttgttgtc accacacgct gtcagcgccg ggacgttttc acactgtgca
    11881 cctgccgcat agcaacgatc gcgaaaagcg cagccgtcag gcaaatgggt gaggtttggt
    11941 accgtccctg gaatggcggg taatagctgg cgtggtactc catgttctgg tgcacattgg
    12001 agcaaaccaa tggtatacgg atgccgggga tgatggataa cgtctgccct cacgccgctt
    12061 tcaattacgc ttccggcata catcacgtaa acgctatcgc acagttgcga caccacggcc
    12121 atatcatggc tgatgaacag aaccgcagtt ccactggcgc gggctttatg tttaagcaaa
    12181 cgcagtacct gcaactgcac cgtgacgtcc agcgccgtag tcggttcgtc ggcaataatc
    12241 aattgcggct cgcaggagaa tgccagcgca atcattaccc gctggcgcat accacctgaa
    12301 agctcaaacg gatagcgcga cataacttcc acggcatccg ggatttgcat ctcttccagc
    12361 aggacaatcg ctttagctct ggcttcccga cgacttattg gttgatgatg gcggatcacg
    12421 tccatcatct gaagacctat tcgacgtgtc ggattgaggg cggtcatcgg ttcctgaaag
    12481 atcatcgcca ctcgtgcgcc gcgccactga cgaagctgtt tttcccgagc gttaagaaca
    12541 tcgtctccca gcagtgaaat ctgcccccga tgtacgcaat aactgcccgt cggtagcaga
    12601 cgcataatca gcattgcggt gactgattta cctgagccgg attctcccac cagaccgaca
    12661 atttcaccgc ggttaatctg caaggacaca ttgttgagcg cgtgaacatc accgttaaaa
    12721 ccggggaaac tcaaatgcag ctgttgaatg tccagaacgg gctgggtcat gactgctttc
    12781 ctcctgcttt cgggtcaagc agatcgcgaa taccatcacc aaagagatta aacccaacgg
    12841 cggtaagcaa aatcgctgcc cttggaaatg cgcaatacca ccactgatcg agtacatagt
    12901 tgcgaccatt cgctaccatc gcgccccatt cagcactcgg ttgttgagca cccaggccaa
    12961 taaatcccaa cgtggcggcc attaaaatcg cgctaccgat atccagcgat gcctgcacga
    13021 tcagcggcgg cagggagtta cgtaaaatat gccagctgat cagatgccaa cgcgacgcac
    13081 caaaggtttt cgccgcctga acataggtat actggcgtac aactaatgct tgcccccgcg
    13141 ccaggcgcac ataaaaggga attcgcacaa tagcaatcgc cagcatggcg ttaaataaac
    13201 tcggcccgag agcggcagcc agtgccattg tcagtaccaa cgaaggaatc gacagcataa
    13261 tgtccatgat gcgcatgatg atggcgtctg cgcgtccgcc aagcacaccg gatagacatc
    13321 cgagtagcga accaatcata cccgcaatgg caaccacgac taatccggcg agaattgatt
    13381 gctgactgcc gaccagtacg cggctgaaca gatcgcgtcc cacttcatcg gtgccaaacc
    13441 agtgcgccgc agaaggcggc aaaaggcggg cggttaaatc aatggcgttg ggatcatgcg
    13501 tcgcaagcca cggtgaaaaa aacatcatca atagcattaa tacaatgatg actgcgccgg
    13561 tcacggtgag cggactgcct ttcagcatcc ataccagttt tgcaccgtta aatcgcgttt
    13621 gtttttgtgg gcgtacggcg gacgtttcct cgcttagcat cattcaccac ctccacgtcc
    13681 gatacgcgga tcaatccaca aatagagcaa atccaccacc aggttgacca gcacataagc
    13741 aaatgaaacc acgacggcaa agcccatcac tgccgggaag tcgagcgcct gtattgatgt
    13801 tactacccat gcacccattc cgggccaggc aaaaacggtt tcggtgagca ctgcgccata
    13861 caacaaatcg cccagcgcca gacccaatac ggtaatcgat gggatcaacg cattgggtag
    13921 cgcataacat aaaacgatat accagccggg caagccgctg gcccgggcgg tacgaatgta
    13981 gtcttcactc aattgttcca gcatcgctga gcggatctgg cgagcgacaa ttcccaggtg
    14041 aacgaacgcc agcgttaatg ccggtaagat gagatgttgc aacgcattga agaagacttc
    14101 accgttgcct tcaagcagcg catcgagcag ataaaagccg gtaacgtgcg ttggtggatc
    14161 cagccagtca tcaagccttc cgccgccggg aagaatttgc agatgaccat aaaacagcac
    14221 aatgacgccc agtccaagcc aaaacgcagg tgtggagatt ccggtaatgg ccattattcg
    14281 caccagatga tccagccagc gatttcgcca gactgcagag agtatgccca gcgggatgcc
    14341 aatcaggagt gccagcagca gggcacaaaa agccagttcc agcgtcgccg ggaaaaatat
    14401 acgcaactct tccaacaccg gacgcccggt acgaatggat gttcccaggt caccatgaaa
    14461 caggtcgctg acgtagcggt aaaactgtac atacagtggc tggtccagtc ccagttgctg
    14521 gcgaatattt tccacgatag catcgctggc gcggtcaccc gccagtaacc gcgccggatc
    14581 gccagggatc agatgcgaga taataaacgt aatcacgcag acgcccgcca tcacgagcac
    14641 cagcccccag cagcgttggc gtaaaatact ccagaaggtc acgttattta ctcatggtat
    14701 tgatattgaa gacctgttcc agcatgggat tgaacacaaa gcctttcacc tctttgttca
    14761 tcgccagttg gtagtttttc tggaacaggt acacataagc agcgtcatca atgacgattt
    14821 tctgtgcctg ctggtagtcc cgggtacgct gcgtctggtc ggtggtcgca agcgcattgc
    14881 gcagtaactt atcgacctca ctgttttcat agaacgagcg gttacccggc agaccttttt
    14941 tgtctgactc aaaccagtaa ttcataaaca tatacgggtc ggcaaaatcc ggactccagt
    15001 tgccaatcgc aatgtcgtaa tcacctttac ccactctgtc gcgcatggtg gcgttcgcca
    15061 gcttttccag cttcacattg atgcccagct tgttgagact ggattgtgtc gccagagcaa
    15121 taggctccca gttcggatcc ttatcggagt agagaaacgt caggctggtg ggtttgctcg
    15181 tcactttatc ccattcggct ttggctttcg tttcgtcatg gttgtattgc attgccgtcg
    15241 catcgtagcc ccacatgcct tccggaatcg ggccgcgcat ctgttttccg ttaccactca
    15301 gaatgccgtt aaccatgccc tgataatcgg tagaccagga aatggcccga cgcagatccg
    15361 cctgattaag aggcgctttg ctgttattca gatacaggta ggtgacgcgc agtgacggat
    15421 actctgccac attgactttg ttttcctgct tcagggcgtt gagttgatcc accggcagcg
    15481 catcggcaat gtcaatatca ccacgggaga gctgcaggcg acgggaggca ctttcaccaa
    15541 taatttttac cgatactcgc ttaaagttcg gtttattgcc agggtaatgc ggatttggca
    15601 ccagaactaa ttgctgacct ttttgccagc ttttcagcat aaatggtccg gaaccggcgg
    15661 tattttgcgc gaggaagccg cgggcatcat ccgctgcatg ttcctttaac accgccggat
    15721 tgataatgga tgcaccgtca ttcgccagcg tgtagaggaa cggtgcgaat ggctggctaa
    15781 gggtaaactt caccgtatgt tcgtcgggag catcaatctt taaatctttg ggaaatgctt
    15841 ctgctggccc ctggccgatt tttagtagcc gctcaaaaga gagttttact gcttcggcag
    15901 tgaccggtgt gccatcggca aatttagcat tgtccttcag ggtgaacgtc cactcttttt
    15961 gatcgtcaga cgctttccag ctacttgcca gatcgccttc aacgtcggtt gagcctttat
    16021 caccgtccgt tttgtactga accagccgct gataagacgg gtaggtcacc gtccagtcgt
    16081 tattatctat tgttaccgcc ggggcgaggg tttgtggatc ggcggcctta ccaatcacca
    16141 gcatatcttt cggtacggcg gcgtgcgcaa ccgggaaagt tgtggcaagg acgagcgcga
    16201 gcaatgtagg acgaaacggt atcgatctct tcatgacagt ttcctgatta actgacgtgc
    16261 tgtgtgccag gggatataaa acaggtgaat tgatcagcga gcagtgggta actcgctgcc
    16321 tggggtaatt cgaagtgcca ccattcgctg gagataccga caaaaccgcc accagtcata
    16381 atcgcattca gcaacagccg attgcgctga gcggcgggcg ggacggaagg gtgataggca
    16441 tgggagcgct cgtgcatttc atcgaaccct gcgcccatat cgaggatgtt cccgtgctca
    16501 tcacgaagcg tcaggtcgat cgccgtgcca cggctgtgat tagaaccgac cgtcacatca
    16561 acaacatatt gcgggtctgg gcaggcttgc cacaacatcg cctgtgcttg ttgtgggcga
    16621 tacgcatcgt aaatcaccag ttgtaagcct gacagctggg cgatactgat acttttcgcc
    16681 agcgcggtaa tcgcatcctt gtgtagcaga caacgcgctt gctgataaat agctttacct
    16741 gtgatgttat cagcgcaggc gtatttcaat tcgatctcca gatcagggaa gattacggct
    16801 aaatcaacca gttcggtggt atccgacata aacggttcct gtataagaca aaaattgctg
    16861 cgctttccgc ttatgcagat ctcatgccat gccgggataa gcgccagaat gctggcttaa
    16921 agttatattt tttaactttt gatcaacatt tgtgcagcgt agtgcagttt tggtgcaaga
    16981 ggggaagtta aggaaggaat ctcccggaat cgtagatgaa atcacagtat ttaagtgacg
    17041 gtgtcacgtt aaatgaaaac ccgcgagtgc gggcgagagg aatttgtcag attttcagcg
    17101 gtaacacgct gctcatccag cctggaattt cttcggcggg tagggggcgg gaaaagaaat
    17161 atccctgaat aacgcgacag tggatcttgc gtagcatctc aaattgctct ttggtttcga
    17221 cgccttccgc cacgacggtt aaattgaggc tttgcccaat gctggtaatg gcttcaagta
    17281 aggcaaggat gcgtttttcg gtcagacaac gatcgacaaa acttttgtca attttgattt
    17341 ccgttaccgg aagactgact aagcgggata atccggaaaa gcccgtacca aaatcatcta
    17401 ccgataagcc cacgcccata tcacgcagga tctgaatgcg cttaaagatt tcggtatcgt
    17461 gttccatcat catgctttcc gtgatttcta ccgtcagctg gtggccgtca ataccccagg
    17521 cgtgcattgc atcagacacc tgattaggca gttgattact gcgaaagtgc agtgccgaca
    17581 agttaacgga aagcgccggg atatgaatat tctggctacg ccattctgct aactgacggc
    17641 aagcttccgc gatgacccag cgcccaatat tttcgatttc accaatctct tctgcgagag
    17701 gaataaaccg tgaagggggc acatgaccat gctggggatc gtgccagcgc gcaagggctt
    17761 cgatgccgta cagttcaccc gtttctgcga agatttgcgg ctggtaaaca agtttcagtt
    17821 ggttattgct aatcgcttct ttcagcgctg cgcctaaaac caaacgctct tttaccattt
    17881 cgttcatcgc cgggctgaag aactgccagc cgttaccgcc attcttgcga atataatcta
    17941 ttgcattgtg agcagtggag agtaggtaat cgtggttttt acccacgtcg tagctgatgc
    18001 caatactcaa ggtaagcggg aatggtttat cgtcaatcat tatcggcttg ctgaccacat
    18061 tccgtagctc atcggcgatc tgggtaatgt tactgacgtc gttttccagg ctcacgagga
    18121 caaactgcgt accttcgata cgacagagat actgatccgg tttgagtttt ccacgaaagc
    18181 gattgaccac ttccagcaat gcctgatcgg cccacgcata gccaaggcta tcaatcacat
    18241 cctgaatatg gtcaacaccg atgagataca ccaccggaga gacggctttg tcgaccaggt
    18301 catcgaggta attgtgcagg ttattgcgat ttggcagatc ggtcatcgga tcaaattgga
    18361 tgagttgttc aatatgctgg cggctttttt cctgttccag cgccagcgcg gccatatgct
    18421 gactgatatc tgccacgcgt tcgataaagg cgctggtttc tgctcctgac gaggttttaa
    18481 tttgcaggat ccccgcaggc gcgccatcac gctgacgaat ggtcgctgac cagctttgcg
    18541 cattttgaac ttctgcaccg tgcgaagatg acgcccagtg tatcggcatc ccgttgcgca
    18601 gtgcgaacag cgaaacatgc gattcgttga gaacagattc gatgttacga caaatgattt
    18661 cccccatttc atgaaatggc gggctgctgc acatggcggc gagaatattg ccttcaagct
    18721 ggcggatttg ccgttcttcg gtgatatccg agaaagtcat taccaggttc tgcagatgcg
    18781 cgagcacgtc ataaaccggg ctgatagagg ctttaatcca gattttttca ccggtgcgcg
    18841 tcaacagcag aaattcgtcc tgatcgcggg cggttttcca tagcaactgt tgtaaacgaa
    18901 tgcggttatc ggcagggaat tcaggaatgt tcaggagtgt atcgggctgc ataccgctgg
    18961 cttcgctaat gcagtaacca aacatttcgg taaatgcgcg attgcactgc acaatatggc
    19021 gttccggatc gaggacaatc accggtcggt cgagatggtc aacggcaata atcaattgtc
    19081 gggtctgttc tttttgcgcc atttctacgc tggcatcccg taccagcgcc aggtaataaa
    19141 ctttcccctc ggcgctcact ttcgatagcg caaaacgggt ccagatttta ctgccgtctt
    19201 ttttctccag ctgcagctcc cgactcatcc cctcgacgcg cgctttacca ccttcacggt
    19261 tatgacgaat gtattcagga tgcgcaggac gcaaatcccg cggaatcagc atatcaatgt
    19321 tattgccaat gacttcttca cgtttgtacc cccagagctt ctctgcggcg gggttgaaaa
    19381 acatcacttc atcattttcg ttaattaaca ccgcgcccat catattttgt tcaagggcgg
    19441 ggaaaaaaat gccatcggcg gcattttccg catcggttag cttcatgatt acctctgcat
    19501 cctggcgcat ctaaagactg gctttccaga gttcaacacg gtttctacct cgtcttttgg
    19561 cgatatacag agcttcatcg gctatttgaa tgaggcgctc atagtcagga tgaccattaa
    19621 acatggcggc accgatggaa agtgagaggg caatatcctc gccgtttgcg gctttcagtt
    19681 tggttttctc cacccgactg cgaatacgtt ctgcggtacg taacgtttcg ttttcagaag
    19741 cttcagtcaa aacaatgata aattcatcgc ccccgtagcg aaaaacataa tcactactgc
    19801 ggacgttgtc ataaaaggcc tgagagactt tacgcagaat ttcatcgcca gtgttatggc
    19861 cccacgtatc gttgatctct ttgaatttat caacgtcaat aatcagcact gacagcggtg
    19921 taccggtccg gttggcatgg gcaatttcgc gtttgaagat agtcggcagg aagcggcggt
    19981 taagtaattt cgtcagtaca tccataccga cttcgtggcg cgatacttct tcaaacaatt
    20041 cacgcagcaa ggtaataatt tgcgatacag tatttcttat ctgtaataaa aatttcaccc
    20101 gcagacttct gttattcaaa ttcctggtgt tacgcatggt ttgattgaat ataccgtcga
    20161 aatcctgaat cagacgggag atatgcctac ttcggcaatg ccactaaaat aatgtcgacc
    20221 tttatggtta aaccacaggc caaaatcagc ctggcttaaa ggcaaactac tgcctaaatc
    20281 agaatccagc aggattttat agataatatc tatttcccat gaaagtattg aggctatttg
    20341 ccgttctttt tcttcttcgg cgttttctag taacgagaag atacgatagt tttcatcttc
    20401 ctttgaggca ctactgtcac taaaggtaaa cgcgcgggtc atcacttcca tagcgatatc
    20461 aatactgtta atcgagaaat ggtagacctg aagtttttct gcggcagaat aatccgaaga
    20521 gaagattacc ggatagagga tttttttcag cacccgaaac cccatctcga caatttctac
    20581 cggaattcct atgcgggcat gcacttccgc gacggtatgc tggatttgta ttagcctttc
    20641 gacatcgtca acctgggcag aaagcacgtt aataatccag cgttccatcg cacttttcaa
    20701 ctgccgctca acttgttcat tactcaagaa ttcttcggca tgcgggtcga tgcggacaat
    20761 tcgataaaac tcgatactca gataatgagc atgcgcaacg gcaatttccg cggctttagc
    20821 acgaatgagt ggatctgccc gttcgacaag tccggtccac tcatctttca ttcttttaaa
    20881 atacatctcc ataattcaca cccttataag gctgggaaat cagacggaat caaaacgcaa
    20941 cgtgcgagat cgactaactg caccatattc tcctgaaata tgaagatata ctgaaaagaa
    21001 ataagcgact taggacagtt tcaatctacg ctactgttct tcagaagagt atagcccatc
    21061 gtaattattt ttcggtgaca gcgaatatcg tatggttttt catattcata catttttatt
    21121 agggatttat agctgtttaa ctaagtgtgg ttaattgcgt ggttaatttg acttatgtaa
    21181 gcatgattac tagtgggata tttcacaagg tgtaacaagc cggtgggtaa agcaccggct
    21241 tgttacaaag taagcatggg agtttaactg ccccaacgac tttgcagata gctgaccgct
    21301 tgttgagtct gcggtttatt cagatagtcc tcacggaaca agatggtgcc gctaatttct
    21361 ggtaccgcat cgttaagatc gagctgcttt ttcagttccg gtacgccgcc gttaatcatc
    21421 cagtctggct ctatctttga aggttcaccc actttataga aggcgatacc gatatacagg
    21481 cgggtcctgg tcggtttaac gacatccgcc caccattttg ccaacacgtc ataacgcgcg
    21541 gcactccgtg agaacggcca gtaaatttgg ggagcaatgt aatccagcaa tccttgttcc
    21601 acccatcgac gggtgtcagc gtaggattcg tcataggctg ccgcgcctcg ggtatcggaa
    21661 ccgagcggat cgtgtgatcg gttacgccac acgcctgccg ggctaacgcc aaattcgact
    21721 cccggcttaa tgcttttaat ggtgtgcgat acttttgtaa ttaactgctg agtattgttg
    21781 cgccgccagt ctgcttttga cgcaaatgcg cctccgtatt tacggtacgt ttcgttatca
    21841 tttagccgtg aacccggtga ctccgtatag aaatagtcgt caaactgcac gccatctacc
    21901 ggatagcggg aaaccacttc ggcgactatt gatgtgatcc agtcctgaac ctcagggatg
    21961 cccgggtcga ggacaaagcg atcgccagac gttctgatcc agtcacggtg ttgcacatag
    22021 acgctcgccg gttgttgaga cagagtgcta ttcagttccc tgatagtacc gggcttcgta
    22081 ttaaccgata cgcgataggg gttaaaccag gcgtgtactt tcatcccacg cttgtgggct
    22141 tcgtcgagca taaattgcag cggatcgtaa cccggatttt caccaatctt accggtcata
    22201 agatcggacc acggcaaaat ttttgatggc cacagggcgg taccgtccgg cttgacctgg
    22261 aaaaagaccg tgtttatacc gagacgttgc agatgatcca gtttgtcgat catcgcctgt
    22321 tgttgtacac gggcccggct ggtggggtta ctaatgttaa ccgaggaaac cggtggccag
    22381 tcgaggcgag acaccgtggc cagccagatg ccacgcatcg gttgtgacga ttgttgcgtc
    22441 gtggctggtg gctttgaacc tgctggtggt gtcaccatgg actctggagg cgtgctttta
    22501 caactacaca gtaaaagtgc cagtgcaacc agtatcgctg gtcttctaat cgttaatttc
    22561 ttgtttcggg agcagatatc catatgtgct ggtttccggt aaacagatgt gcgctcattc
    22621 tcgtacttat cccggttaag tcaatacgac agcaagcacg aaaaagggag cgatgaatta
    22681 tcgctccctt gtcttataac cattcagaca tggttagtgt ttcttgtcat tcatcacaat
    22741 atagtgtggt gaacgtgcac gcgggtgcag gaagaagtga ccttttggtg cgttctgact
    22801 gttgattggc tccagagtga cgccggtatt cgctttgcct ttacgatcat gaacagcata
    22861 gagaataaag ggcagggcaa gtaccaccag gaaactaacg accagtaatt caacatacat
    22921 atcggtagag tcaccctgga tgttatccgg cggcaggaag gaaacaataa acgccataat
    22981 tgaagtcagc agaccgacaa ttgccacgac cagttttacc cctttaccac cagggatatt
    23041 aaatgtgcgt tttaagtcag gatgtttaag aactaacaca atgtagccaa taaacagcat
    23101 gaaataagca cacagataaa tcaccaccgt cagcgccagt gcgatcagga aggacatgtt
    23161 gttaccgcca ccggtattgg tgaggatgat caacgcgata gaagtaatca ccagctgcga
    23221 aatgaccagc gttaccggta cgccattttt gttcatttta gcgaatgccg ctggcagcag
    23281 gtttttctgc gctgtgacat acatcccgcg agaaggacca acaatccagg aggcgatttc
    23341 cgccagaaca cccagcaaca gcagtgcgga gatcacgcga accgtccact caatttccgg
    23401 tgccacatgg gacatcagaa cggtaaaggt ttgcattacc cctgcggaga ggttgatttc
    23461 attacccgga atgaccatcg caatagataa accgccaaca gagcttaagc agattgccgc
    23521 caccatcagc agtaacatag ccagtggata gtcgcgcccg gggttgctca tttcattgac
    23581 gtgagttgcg gaagcttcta cgcccatata actcaaaatg aaggcaacaa atacaaccag
    23641 tgtgcccact ttagagaagt cagggaagaa ggtcttcgaa tccatttcga tagcaacggg
    23701 ggcaccggag tgcagataaa tagccgctaa tgcgatcaaa ataaatgcag gtaacaggat
    23761 accggcgaag aagccaactt tagcaattcg cgccgtgtat ttcgtgccac caaactgcgt
    23821 taatgccagc gcccaaagaa tgatgagtgc tgcaatagtt ttggtaatgg ggtcttcatt
    23881 cagcgctggc catttcagga tgtaggagag tgcccctaac acgaaataga gcatcggaat
    23941 aaaaccaatg gcgatttgca gatagccaaa tgagatcgct gcaaatcccc atctcggccc
    24001 cagagtattt gatacccagg cgaagacacc accttcttcc cagccgtcga cggtggccat
    24061 ttccgcagca caaagtccca cgggaataaa ccataaaatc ccgcctaata gcaggaagaa
    24121 gactaatgaa aagcccgatg ttgcgaaggt agggtattca taaacagcca ttaccatcga
    24181 tgccgttatg gcaaagaatc cgagtaatgt gagctgctta gctttacctg tctgtactga
    24241 tgtagccata ttatccccct aaaacggtat tcctgtcgga accgcacctg ttttgttctg
    24301 aagcgtattc agaacaatat tttccgttgc taatgccagt gaacagactt tggaaattgt
    24361 cccgaaacgg gttcgtttcg ggacaccgtt accgttaaac gttatcaggt atgtttaaag
    24421 ctgttctgct gggcaatacc ctgcagtttc gggtgatcgc tgagatattt cagggaggct
    24481 ttgtagtctt ccagcaacag ttcagcaaag tccatttcga agccgcgacg acacataat
//
LOCUS       DAJTSL010000051        24417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTSL010000051 DAJTSL010000000
VERSION     DAJTSL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.051"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..326)
                     /locus_tag="OO433_001828"
     CDS             complement(<1..326)
                     /locus_tag="OO433_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050331.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFV"
     gene            complement(442..993)
                     /gene="maa"
                     /locus_tag="OO433_001829"
     CDS             complement(442..993)
                     /gene="maa"
                     /locus_tag="OO433_001829"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR6050332.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(1165..1383)
                     /gene="hha"
                     /locus_tag="OO433_001830"
     CDS             complement(1165..1383)
                     /gene="hha"
                     /locus_tag="OO433_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6050333.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1409..1783)
                     /gene="tomB"
                     /locus_tag="OO433_001831"
     CDS             complement(1409..1783)
                     /gene="tomB"
                     /locus_tag="OO433_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6050334.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2329..5478)
                     /locus_tag="OO433_001832"
     CDS             complement(2329..5478)
                     /locus_tag="OO433_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6050335.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5501..6694)
                     /gene="acrA"
                     /locus_tag="OO433_001833"
     CDS             complement(5501..6694)
                     /gene="acrA"
                     /locus_tag="OO433_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6050336.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDNKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTAPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            6836..7483
                     /gene="acrR"
                     /locus_tag="OO433_001834"
     CDS             6836..7483
                     /gene="acrR"
                     /locus_tag="OO433_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6050337.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7611..10973
                     /gene="mscK"
                     /locus_tag="OO433_001835"
     CDS             7611..10973
                     /gene="mscK"
                     /locus_tag="OO433_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6050338.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLELQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIVGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYIDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(11185..11346)
                     /gene="rsmS"
                     /locus_tag="OO433_001836"
     CDS             complement(11185..11346)
                     /gene="rsmS"
                     /locus_tag="OO433_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6050339.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(11360..11887)
                     /gene="priC"
                     /locus_tag="OO433_001837"
     CDS             complement(11360..11887)
                     /gene="priC"
                     /locus_tag="OO433_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6050340.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            11957..12334
                     /locus_tag="OO433_001838"
     CDS             11957..12334
                     /locus_tag="OO433_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6050341.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12487..13038
                     /gene="apt"
                     /locus_tag="OO433_001839"
     CDS             12487..13038
                     /gene="apt"
                     /locus_tag="OO433_001839"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6050342.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13167..15038
                     /gene="dnaX"
                     /locus_tag="OO433_001840"
     CDS             13167..15038
                     /gene="dnaX"
                     /locus_tag="OO433_001840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6050343.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVPSALEKAP
                     AKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAARVS
                     QLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSTLKGSTVEL
                     TIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI"
     misc_feature    14431..14495
                     /gene="dnaX"
                     /locus_tag="OO433_001840"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            15091..15420
                     /locus_tag="OO433_001841"
     CDS             15091..15420
                     /locus_tag="OO433_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6050344.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15420..16025
                     /gene="recR"
                     /locus_tag="OO433_001842"
     CDS             15420..16025
                     /gene="recR"
                     /locus_tag="OO433_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6050345.1"
                     /translation="MQTSPLLTQLMEALRCLSGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16135..18009
                     /gene="htpG"
                     /locus_tag="OO433_001843"
     CDS             16135..18009
                     /gene="htpG"
                     /locus_tag="OO433_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6050346.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18190..18834
                     /gene="adk"
                     /locus_tag="OO433_001844"
     CDS             18190..18834
                     /gene="adk"
                     /locus_tag="OO433_001844"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6050347.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            18966..19928
                     /gene="hemH"
                     /locus_tag="OO433_001845"
     CDS             18966..19928
                     /gene="hemH"
                     /locus_tag="OO433_001845"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6050348.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEYIEMMANLVAAYR"
     gene            complement(19925..20884)
                     /gene="aes"
                     /locus_tag="OO433_001846"
     CDS             complement(19925..20884)
                     /gene="aes"
                     /locus_tag="OO433_001846"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR6050349.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            21036..22340
                     /gene="gsk"
                     /locus_tag="OO433_001847"
     CDS             21036..22340
                     /gene="gsk"
                     /locus_tag="OO433_001847"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6050350.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22473..24149)
                     /locus_tag="OO433_001848"
     CDS             complement(22473..24149)
                     /locus_tag="OO433_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6050351.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         6069 a         6361 c         6280 g         5707 t
ORIGIN      
        1 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
       61 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
      121 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
      181 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
      241 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
      301 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
      361 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatctacc acaattttgc
      421 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
      481 gacaacgttg tccgggacac cttttgtgac aactgcacct gaagctacca cgacgttatc
      541 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
      601 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
      661 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
      721 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttgccgagaa aaatgttata
      781 gccataatca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaataaatc
      841 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
      901 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
      961 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
     1021 gcaacacagt aagacgaaaa gtcagtgaac agaacgttcc cattacatct gtgtgataaa
     1081 gatcacatag ggtatgtctt catggtgaaa aagtataaaa ttcttaataa acagccggtt
     1141 atagctccga aagcgaatta tttattagcg aataaatttc catactgagg aagggatctt
     1201 gtcgtatagt ttattcatgg tcaattcggc gaggcggtga tctgcggctg agtaaaatac
     1261 cgccagttca ttatctgata attcgtattt atttttctcg ataacacgct ccagcgtgtc
     1321 aattgtctgg caacgacgta aacgcattaa ataatcggtt ttcgttaaag gtttttcgga
     1381 catacttcta cccatggttg taataattct aacaagataa actcgcagga ttctctttcg
     1441 tcgcattgac aaaacaacgg aatagtcgat tacctgactt ccgccatttc tgaagatcct
     1501 gcatattaat accataacta ctgaacaaca taaaggtgtc atccagatat tcgtcgatct
     1561 gctcaatgag cttattgtct tcattatact taattttgta attaagtgcg aaggtcgcaa
     1621 tatgctcaat cagttcattc aactggaggt tgatcgccga ggttgggtcg ttaacccagc
     1681 cataattgct ttcttcaagg tttgcaaggc agtcatgata cagggtttca cagagaaact
     1741 taagctgtgc gatatcatgt cttttgggtg agtattcatc cataacgcgt ccccttctta
     1801 gcggttgaac taacggacac ctttcgggat ggaaaaaact tactgacctg gacttgccct
     1861 ctcgtttgtt agcttaacta taagccactc tttgcaggta ttcatcgcat tattacgaaa
     1921 aattacaatt aggaaggaat cgggacagga ctcacttttt catactagca ctaacccgca
     1981 gcaggtaaaa gcagtttgct cgaactgttc ataacatttc aggattatcg ccagatgaaa
     2041 ttggtgacac taataccagg attgctctga atatgacgta ataaccgagg aatgaataaa
     2101 gaattaccgc aaataattaa gaatagcctc taaatgatta tggattatta aatctattag
     2161 cgctacttaa tataaattaa ctaacaatca gtatttttta tgaattttat ccgtggttaa
     2221 tactggtttt cgtatgagat cctgagttgg tggttcaatt actccttaat gttcgtaggt
     2281 tatgcataaa aaaggccgct tgcgcggcct tagtgattac acgttgtatc aatgatgatc
     2341 gacagtatgg ctgtgctcga tatcttcatt cttgcggcta aagcggcggc gaaccaccac
     2401 aaagaatacc ggaacgaaga agattgccag taccgttgcg gtcaccatcc cgcccattac
     2461 accggtacct actgcgttct gcgcgccgga accagcacca gtactgataa ccagcggcat
     2521 aacgccgagg ataaacgcca gcgaggtcat caggatcgga cgtaaacgca tccgcactgc
     2581 atcaagcgtc gcttcaatca gacctttacc ttctttatcc atcaagtctt tggcgaattc
     2641 gacgataagt atcgcgttct tcgccgacaa cccaatggtt gtgagcaggc ctacttggaa
     2701 gtaaacgtca ttggtcaggc cacggaaggt ggcagccagc aacgcaccga taacccccag
     2761 cggaacgacc agcataacgg agaacggaat cgaccagctc tcgtacagcg ccgctagaca
     2821 caggaatacg acaatcaacg aaatcgcgta cagtgaaggt gcctggttgc cggagaggcg
     2881 ttcctgatag gacatccccg tccagtcata gccaacaccg gtaggcagtt tgctcgccag
     2941 ttgttccatt agctccattg cttcaccggt acttttaccc ggtgccgcct ggcctaagat
     3001 ttccatggat ggcaggccat tgtaacgttc cagacgcggc gaaccgtact cccaacgaga
     3061 agaggagaat gccgagaacg gcaccatctg accatcagca gcacgaacat accagtcgcc
     3121 gatatcatcc ggcagcatac ggtatttcgc ttctgacatg acgtaaactt tcttcacacg
     3181 accgcggtcg ataaagtcgt tcacatagct accgccccat gcagcgccca gagtggtgtt
     3241 aatgtcgttg atagaaacac ccagcgcctg cgctttttcc tggtcgatat caatcttaaa
     3301 ctgcggggta tcttccagac cgtttggacg tacgctggtc aacatatcag gatgctttgc
     3361 tgcttctgca agcagctggt tacgcgcctg agtcagtttt tcgtgaccaa ggccagcctg
     3421 gtcaatcagc tcaaagtcaa agccggttgc agtacccagt tccacgattg cgggcaggtt
     3481 aaaggcgaaa accatcgcat ctttgatttg cgagaaagcg cgtgttgcac gcatggtaat
     3541 cgcttcaact ttgttttctt cgcccggacg atcggcccag tccttcaagg aaacgaacgc
     3601 aataccggta ttctgaccac gtcccgcaaa gccgaagccg ttaacggcga acaccgactc
     3661 aacgttgttc ttttctttgg tcagatagta atgcgttacc tcattgagca ctttctgcgt
     3721 acgttcctgc gttgcacctg ctggcagctg aaccatggtc ataaatacgc cctggtcctc
     3781 atctggcaag aaggagcttg gcagacgcac gaacagatag gccatgccga ccacgatgat
     3841 cagatacagc accaggtaac gccccgtact gcgcagaata ccgcctacgc tgtcggtgta
     3901 gtggtgcgtg ctcttctcga acatgcggtt aaaccagccg aagaagcctt ttttaccttc
     3961 cccgtgatcg cctttggcaa tcggtttcag catggtggca caaagagccg gagtcaggat
     4021 caacgctacc agtaccgaca gcgccattgc tgaaacaatg gtaatagaga actgacgata
     4081 gatagcacca gtagaaccgc caaagaaggc catcggtacg aataccgctg acagtaccat
     4141 cgcgataccg accagagcgc cctgaatctg ccccatcgac ttacgggtag cttcttttgg
     4201 cggcaaacct tcttccgcca taacacgctc cacgttttct accacaacga tagcgtcatc
     4261 caccaacaag ccgatggcga gcaccatccc gaacattgtt agcgtgttta tcgagaagcc
     4321 aaaggcggca aggacggcaa aggtcccgag caataccacc ggtacggcaa tggtcggaat
     4381 caacgtcgcg cggaagttct gcaggaacag atacataacc aggaacacga ggatgatcgc
     4441 ttcgaccagc gttttaacca cttcgtgaat agagattttc acgaacggcg tggtgtcgta
     4501 cgggtaaaca attttcagac ccgacgggaa gaacggttcc atcttcgcca gttcagcacg
     4561 gattgccgca gcggtatcca gcgcatttgc accggtcgcc agcttgatcc ccagaccgga
     4621 agccggttgg ccgttaaact ctgcgatgat gtcgtagttc tcaccaccca gctcaatctt
     4681 cgccacatca cgcagcagga cgcgggaacc atcctgattc actttcagca ggattttgcc
     4741 gaactcttca gtagaggtca gacgcgtctg agcaataata gaggcgttaa gctgttggcc
     4801 tttcaccggc ggcgtaccac cgagctgacc cgccgcaacc tgggcgttct gcgctttgat
     4861 ggcggtaatg acatcaaccg gcgttagctg gaatttgttc agctcattcg ggttcatcca
     4921 gatacgcatc gcgtactgtg aaccgaacaa ctgaacatca cccacgcccg acgtacggct
     4981 gatggcatct ttcatattcg ccgctacgta gtcagagata tcctcctgcg tcatggtgcc
     5041 atcggtgttg ataacgccga caaccatcag gaagctgctg gatgatttct caacgctcac
     5101 cccttgctgc tgaacttctt gcggcagcaa cggcatcgcc agctgcagtt tgttctgcac
     5161 ctgaacctgc gcgatatccg catcagtacc agactcaaag gtcagggtga tctgcacggt
     5221 ccccgtggag tcactgttag aggacatgta catcaggtta tcgataccgt tcatattctg
     5281 ttcgataacc tgtgtcaccg tgtcctgcac tgttttcgca tcagcgccgg ggtaggaggc
     5341 ggagatcgtt actgccggcg gtgcaatcgt aggatattgc gccaccggca gtttgaggat
     5401 cgccagcccc cctgccaaca tgatgataat ggcgatcacc cacgcaaaaa tcgggcgatc
     5461 gataaagaaa ttaggcatgt cttaacggct cctgtttaag ttaagacttg gactgttcag
     5521 gctgagcacc gcttgcggct tgctggttat tatcagcggt aacttcttgt gcttttacct
     5581 ggacaccagg acgcactttc tgcagcccac ttattactac gcgatcgcct gctttcagac
     5641 cttctgtcac cagccactta tcgccaatag cctggcttgc aacgatcgga cgggtttcca
     5701 ctttgtcatc cgcgccaact accagtacgg tggcatcacc acgcggcgta cgggttacgc
     5761 cctgttgcgg gactaaaata gcgtttggat taagcccttc ttccagacgt gcgcgcacga
     5821 acatacccgg cagcagagtg tgatccgggt tcgggaagat agcgcgtagg gtgatagacc
     5881 cagtggtctg atcaacggta acgtcagaga attccagcgt accgtcctgc gggaacttaa
     5941 tgccgtcact ggtgatcagc gacactttgg ctttgccgtt ctcttgtttc agcgtgccat
     6001 tcgccagttc ctgtttcagg cgcaggaagt cgttgctgga ctgggtcaca tcaacgtaga
     6061 tcggatcaag ttgctgcacg gttgccagcg cagtcgcctg accgttctgt accaatgcgc
     6121 cttccgtcac gttcgactta ccaatgcgac cgctaatcgg agcggtgact ttggtgtaag
     6181 ccagattgat ccgcgcagtt tcaacggcag ctttcgccgc agttaccgca gcattcgcct
     6241 gttgcgcatc agccagagcc tgatcgtact cttgcttact gatgtactga gtaccgagca
     6301 gtttctgata acgattcacc gtcaattgcg cgatattggc tgcagcctgg gctttcgcca
     6361 gatcaccttt cgcactgtcg tatgtcgcct gataggtcgc aggatcaatc tgatagagag
     6421 agacaccagc ttcgatgtcg ctaccttctt tgaaattacg cttcaggata atcccgctaa
     6481 cttgaggacg aacttctgcg atccggtagg cactggtgcg acccggaagc tcggttgtga
     6541 tctgcagagg ttcagttttg actgttacta cgccaacggc gggcatctgc tggccacctt
     6601 gttgggcctg tttgttgtca catcctgtta gggctaagct gcctgagagc atcagaacga
     6661 ccgccagagg cgtaaaccct ctgtttttgt tcatatgtaa acctcgagtg tccgatttca
     6721 aattggtcaa tggtcaaaag ttaataaacc cattgctgcg tttatattat cgtcgtgcta
     6781 tggtacatac attcacaaat gtatgtaaat ctaacgcctg taaattcacg aacatatggc
     6841 acgaaaaacc aaacaagaag cgcaagaaac gcgccaacac atcctcgatg tggctctacg
     6901 tcttttctca cagcaggggg tatcatccac ctcgctgggt gagattgcaa aagcagctgg
     6961 cgttacgcgc ggtgcaatct actggcattt taaagacaag tcggatttgt tcagtgagat
     7021 ctgggaactg tcagaatcca atattggtga actagagctt gagtatcagg caaaattccc
     7081 tggcgatcca ctctcagtat taagagagat attaattcat gttcttgaat ccacggtgac
     7141 agaagaacga cgtcgattat tgatggagat tatattccac aaatgcgaat ttgtcggaga
     7201 aatggctgtt gtgcaacagg cacaacgtaa tctctgtctg gaaagttatg accgtataga
     7261 acaaacgtta aaacattgta ttgaagcgaa aatgttgcct gcggatttaa tgacgcgtcg
     7321 ggcagcaatt attatgcgcg gctatatttc cggcctgatg gaaaactggc tctttgcccc
     7381 gcaatctttt gatcttaaaa aagaagcccg cgattacgtc gccatcttac tggagatgta
     7441 tctcctgtgc cccacgcttc gtaatcctgc cactaacgaa taaccctgaa tctgactcca
     7501 ggatttttcc tggacatttt cgtcgttgct attctggttc actgcgtcgt gatattcttg
     7561 cggtttgact ttttcaggtc gttcttcagg ttcagaaacc ttcattcatc atgactatgt
     7621 tccagtatta caaacgatca cggcattttg ttttttcagc atttattgct tttgtttttg
     7681 tcttgttatg ccagaacacg gcgtttgcgc gggcgtcatc gaatggtgat ctgccgacaa
     7741 aagcggacct gcaggcgcaa cttgactcac taaataaaca aaaagatctt tctgctcagg
     7801 acaaactgga gctgcaggat ctgacagata cattagccac cctcgataaa atcgatcgca
     7861 taaaagaaga gacagttcag ctacggcaaa aagtcgctga agcgccggaa aaaatgcgcc
     7921 aggcgaccgc ggcgttaaca gcacttagcg atgtcgataa cgacgaagaa acgcgcaaaa
     7981 ttctgagtac gctttcgttg cgccagctgg aaactcgcgt tgcccaggcg ctggacgatt
     8041 tgcaaaacgc acaaaacgat ctggcgtctt ataacagcca gctggtttcg ttacagacgc
     8101 agcccgaacg cgtgcaaaat gcgatgtata acgcttcgca gcagctgcaa caaattcgca
     8161 gtcgtctgga tgggactgat gtcggcgaga ctgccttacg tcccagccag aaagtgttaa
     8221 tgcaggctca gcaggcgttg ctgaatgcgg agattgacca gcagcgtaaa agcctggaag
     8281 ggaacaccgt cttgcaggat accttgcaaa agcaacgtga ttacgtgacg gcgaacagcg
     8341 cccgtctgga gcaccagttg cagctgttgc aagaagcggt aaacagcaag cgcctgactt
     8401 taactgaaaa aacggcgcag gaagccgtct ccccggatga agccgcgcgt attcaggcta
     8461 atccgctggt gaaacaggaa ctggaaatta accagcagtt aagtcagcgt ctgattaccg
     8521 cgactgaaaa cggtaatcag ttgatgcagc aaaacattaa agtcaaaaac tggctggagc
     8581 gggcgctgca atcggaacgc aatattaaag agcagattgc cgtcctgaag ggcagcctgc
     8641 tgttgtctcg tatcctttac cagcaacaac aaacgctgcc ctcggcggat gaactggaaa
     8701 acatgaccaa ccgcatcgcg gatttgcgtc tcgaacagtt tgaagttaac cagcagcgtg
     8761 atgcactctt ccagagcgat gcgttcgtca acaaactgga agaaggtcac accaacgaag
     8821 tcaacagcga agttcacgat gcgttattgc aagtggttga tatgcgtcgc gaattgctgg
     8881 atcaactcaa caaacagttg ggtaaccagc tgatgatggc cattaacctg caaatcaacc
     8941 agcagcagtt aatgagcgta tcgaaaaacc tgaaatccat cctgactcag caaatctttt
     9001 gggtgaacag taaccgtcca atggactggg actggatcaa agcgttcccg caaagcctga
     9061 aagatgaatt taagtcgatg aaaatcacgg tgaactggga aaaagcctgg cctgccgttt
     9121 ttatcgcttt cctcgccggt ttgccgctgc tgttgattgt cgggctgatc cactggcgtc
     9181 tgggctggct gaaagcgtat caacaaaaac tggcttccgc tgtgggttcc ctgcgtaacg
     9241 atagccagct caacacacca aaagcgatcc ttatcgacct gatccgcgcg ctgccggtgt
     9301 gcctgattat tctcgcggtt ggcttgattc tgttgaccat gcagctcaac atcagcgaac
     9361 tgctatggtc gttcagcaaa aaactggcga ttttctggct ggtgtttggc ctgtgctgga
     9421 aggtactgga gaagaacggc gttgccgtgc gtcacttcgg catgccggaa cagcagacca
     9481 gccactggcg tcggcaaatt gtccgcatca gtctcgcatt gctgcctatc catttctggt
     9541 ctgtggtggc agaactttcc ccgctgcatc tgatggatga tgtgctgggg caagcgatga
     9601 ttttcttcaa cctgctgctg attgccttcc tggtatggcc aatgtgccgc gaaagctggc
     9661 gtgataaaga gtcgcacacc atgcgactgg tcaccattac cgtgctgtcg attatcccga
     9721 ttgcattgat ggtgctgact gccacgggtt atttctacac cacgctgcgc ctctccgggc
     9781 gttggattga aaccgtttat ctggtgatca tctggaactt gctctaccag acggtattgc
     9841 gcggcttaag cgtagcggcg cggcgtatcg cctggcgtcg tgcgctggcg cgtcggcaga
     9901 atctggtgaa agagggcgca gaaggtgccg aaccgccgga agaacccacc attgcactgg
     9961 aacaggttaa ccagcagacg ctgcgtatta ccatgttgct gatgtttgcg ctgttcggtg
    10021 tcatgttctg ggcaatttgg tccgatttga tcaccgtgtt cagctatatc gacagcatta
    10081 cgctctggca ttacaacggt actgaagctg gcgctgcggt ggtgaaaaac gtcaccatgg
    10141 gcagtctgtt gtttgcgatt atcgcctcaa tggtggcctg ggcgttgatt cgcaacctgc
    10201 ctggtttgct ggaagtgctg gtgctctcgc gactgaatat gcgccagggc gcgtcgtatg
    10261 ccattactac catccttaac tacatcatta ttgccgttgg tgcgatgacg gtgttcggat
    10321 cgctgggcgt ctcttgggat aaactccagt ggctggcggc agcattatcc gtaggtcttg
    10381 gttttggttt acaagaaatt ttcggtaact tcgtctccgg tttgatcatt ctgttcgaac
    10441 gtccggtgcg tattggcgat acggtaacca ttggtagctt ctcggggacg gtaagtaaga
    10501 tccgtattcg tgcgacaacg attaccgatt tcgatcgcaa agaagtgatc atcccgaaca
    10561 aagcgtttgt taccgagcgt ctgatcaact ggtcgttgac tgacactact acgcgtctgg
    10621 tgatccgtct cggcgtggcc tatggctccg atctggaaaa agtgcgtaaa gtgttactga
    10681 aggcggcgac tgagcaccca agggtgatgc acgaaccaat gccggaagtc ttctttacgg
    10741 cgtttggtgc cagcacgttg gatcatgagc tgcgtctgta tgtgcgtgaa ctgcgtgacc
    10801 gtagtcgtac tgtcgatgag ctgaaccgta ctatcgatca gctgtgccgt gaaaacgaca
    10861 tcaacattgc ctttaaccag cttgaagtgc atctgcacaa cgagaagggc gatgaggtga
    10921 cggaagtaaa acgcgactac aaaggcgatg acccgacgcc agcggtaggg taataaccaa
    10981 agggggcgac atttagttgc cccgagatta ctgacaaagt gcgcgttgtt catgccggat
    11041 gcggcgtgac cgccttatcc ggcctacgaa accgcaagaa ttcaatgtat tgcaggagcg
    11101 gtgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgcccaca atcttagggg
    11161 acatttagcg gccccattta tttctcactt ttccgcctca tcatcgcgcg ttaatttctt
    11221 ttcataatcg cgttttacaa tatccagcgc gcgcagcacg gtgcgggctg ggatctgatt
    11281 ttcttccagc agcacaatca aatcgacagc cagtttgaca tcgtcagggg cattttccag
    11341 tgacatactc tctccattgc tagcgggtta agcgcgctag cctgttttcg attttttcca
    11401 gcgcatggcg gcagcgtgcc aggcgcgctt cataggcttc cacttcacga tgcagcgttt
    11461 gctgttccac gagatcggtc acccgcgcca ggcgggctct gcgttcggca accatctcac
    11521 gtagccgccg ctcaaaatcc tgatgctgaa tacgtttacg ctgccagcgg gcaattttcg
    11581 gtggcgcact gtcccactcg cgcaatgacc aggcggaggc ttcacgcgcg atggcttcca
    11641 gttgtgccgc cagatgttcc gccagccagg ccacttgcgg cagctgttgc tgctcaactg
    11701 catgacgaag cgcagccaga ttatcgccag cctcatcgag acaagcctgc agtgttgtcg
    11761 tacgggtttg aaaaaggtgt ctgtcaaaac gggcacttag cgtggcaaat tgcgctaccg
    11821 gggcacaacg ctgacgcagc gtagcgagct gtccttccag tttttccagc agcagggcgg
    11881 ttttcacgat aagacctcaa tgaaaatgat aattgttatg ctaaagtagc cactctgtaa
    11941 gctgacaaca ctcaatatgc aacgaatcat tttaatcatc attggctggc tggcggtagt
    12001 gctgggtacg ctgggcgtgg tattaccggt attaccgacg acgccgttta tcctgctggc
    12061 ggcctggtgc tttgcccgtt cttccccgcg ctttcacgcc tggttgctgt accgctcatg
    12121 gtttggcagc tatctacgtt tctggcagaa acatcatgcg atgccgcgcg gcgtcaaacc
    12181 gcgggcgatt ttgcttattt tgctcacgtt tgccatttct ctgtggttcg tccagatgcc
    12241 atgggtgcgc atcatgttgc tggtaattct cgcctgtttg cttttctata tgtggcgaat
    12301 tccggtgatt gatgaaaagc aagaaaagca ctgaagcaca acaatcgcag ttgcaattat
    12361 tgcgtacagc cagtacattc tggcgttttc gagcacaggc gcaggcggtc aaaggttaaa
    12421 caactgttac ttttgatacg tttaaaacgc gccgtgagta ccaccgtaac aagcaggcat
    12481 acacttatga ccgcgactgc acagcagctt gagtatctca aaaatagcat caaaagcatt
    12541 caggactacc caaaacccgg cattcttttc cgcgatgtca ccagcttact ggaagacccg
    12601 aaagcttacg ctctcagcat cgacttgctg gttgagcgtt acaaaaatgc gggcattacc
    12661 aaagttgtcg gcaccgaagc gcgtggcttc ttgtttggcg ctccggtagc tctgggtctg
    12721 ggcgttggct ttgtaccggt ccgtaaaccg ggcaaactgc cgcgtgaaac catcagtgaa
    12781 acttacgacc tggaatacgg caccgatcag ctggagatcc acgttgatgc catcaaaccg
    12841 ggcgacaaag ttctggtggt ggacgatctg ctggcaaccg gcggcactat cgaagcgacc
    12901 gttaaactga tccgtcgtct gggtggtgaa gtggctgacg ctgcgttcat tatcaacctg
    12961 ttcgatctcg gcggcgaaca gcgtctcgaa aaacagggca ttaccagcta cagccttgtc
    13021 ccgttcccgg gccattaatt atcgccagtc ttgtgctgcc cacgctacgg acagcacaag
    13081 atgtgcattc agcctcgccg ttctgacggg gctgtgttag cattacccct tcgtgaatcc
    13141 accttccagc gtttcagagc ctgccaatga gttatcaggt cttagcccga aaatggcgcc
    13201 cacaaacctt tgctgacgtc gtcggccagg aacatgtgct gaccgcactg gcgaacggct
    13261 tgtcgttagg gcgtattcat catgcttatc ttttttccgg tacccgtggc gtcggaaaaa
    13321 cctctatcgc ccgactgctg gcgaaggggc taaactgcga aaccggcatt accgcgacgc
    13381 cgtgcggcgt gtgcgataac tgtcgtgaaa tcgagcaggg gcgctttgtc gatctgattg
    13441 aaatcgacgc cgcctcgcgc accaaagttg aagatacccg cgacctgctg gataacgtcc
    13501 agtacgctcc ggcgcgtggt cgtttcaaag tttatctgat cgacgaagtg catatgctgt
    13561 cgcgccacag ctttaacgca ctgttaaaaa cccttgaaga gccgccggag cacgttaagt
    13621 ttctgctggc gacgaccgat ccacagaaat tgccggtgac gattttgtcg cgctgtttgc
    13681 aatttcatct caaggcgctg gatgtcgagc aaattcgcca tcaacttgag cacatcctca
    13741 acgaagaaca tatcgctcac gagccgcggg cgttgcaatt gctggcgcgc gccgctgaag
    13801 gcagcctgcg agatgcctta agtctgaccg accaggcgat tgccagcggt gacggccagg
    13861 tttcaaccca ggcagtcagt gcgatgctgg gtacgcttga tgacgatcag gcgctgtcgc
    13921 tggttgaagc gatggtcgag gccaacggcg agcgcgtaat ggcgctgatt aatgaagccg
    13981 ctgcccgtgg tatcgagtgg gaagcgttgc tggtggaaat gctcggcctg ttgcatcgta
    14041 ttgcgatggt acaactttcg cctgctgcac ttggcaacga catggccgcc attgagctgc
    14101 ggatgcgtga actggcgcgc accataccgc cgacggatat tcagctttac tatcagacgc
    14161 tgttgattgg tcgcaaagaa ttaccgtatg cgccggaccg ccgcatgggc gttgagatga
    14221 cgctgctgcg cgcgctggcg ttccatccac gtatgcctct gcctgagcca gaagtgccgc
    14281 gccagtcctt tgcacccgtc gcgccaacgg cagtaatgac gccaacccag gtgccgccgc
    14341 aaccgcaatc agcgccgcag caggcaccga ctgtaccgct cccggaaacc accagccagg
    14401 tgctggcggc gcgccagcag ttgcagcgcg tgcagggagc aaccaaagca aaaaagagtg
    14461 aaccggcagc cgctacccgc gcgcgtccgg tgccatcggc gctggaaaaa gcgccagcca
    14521 aaaaagaagc gtatcgctgg aaggcgacca ctccggtgat gcagcaaaaa gaagtggtcg
    14581 ccacgccgaa ggcgctgaaa aaagcgctgg aacatgaaaa aacgccggaa ctggcggcga
    14641 agctagcggc agaagccatt gagcgcgacc cgtgggcggc acgggtgagt caactttcgc
    14701 taccaaaact ggtcgaacag gtggccttaa atgcctggaa agaggagagc gacaacgcag
    14761 tatgtctgca tttgcgctcc tctcagcggc atttgaacaa ccgcggtgca cagcaaaaac
    14821 tggctgaagc gttgagcacg ttaaaaggtt caacggttga actgactatc gttgaagatg
    14881 ataatcccgc ggtgcgtacg ccgctggaat ggcgtcaggc gatatacgaa gaaaaacttg
    14941 cgcaggcgcg cgagtccatt attgcggata ataatattca gaccctgcgt cggttcttcg
    15001 atgcggagct ggatgaagaa agtatccgcc ccatttgatc gtaagcacag cttacgttcg
    15061 tcatccttaa cgtgattgag agagaaacct atgtttggta aaggcggtct gggtaacctg
    15121 atgaagcaag cccagcagat gcaagaaaaa atgcagaaaa tgcaggaaga gatcgcgcag
    15181 ctggaagtca ccggcgaatc tggcgcaggt ctggtaaaag tgaccatcaa cggtgcacac
    15241 aactgccgtc gcgtagagat cgacccgagc ctgctggaag acgacaaaga gatgctggaa
    15301 gacctggtgg ctgcagcatt caacgacgca gcacgtcgta ttgaagaaac gcagaaagaa
    15361 aaaatggcct ctgtatcctc cggaatgcag ctgccgcctg gctttaagat gccgttctga
    15421 tgcaaaccag cccgctgtta acacagctta tggaagcact gcgctgtctg tcgggcgttg
    15481 gcccgaagtc ggcgcagcgt atggcgttca cgctgcttca gcgcgatcgt agcggcggga
    15541 tgcgtctggc gcaggcgctc acccgggcga tgtcggaaat cggccactgc gccgattgcc
    15601 gcactttcac cgaacaggaa gtctgtaaca tctgttcgaa tccgcgtcgt caggaaaacg
    15661 gtcaaatctg tgtggtggag agtccggcgg acatctacgc cattgagcag acggggcagt
    15721 tttctggtcg ttattttgtg ttgatggggc atctgtcacc gctggacggc atcggtccgg
    15781 atgatatcgg gcttgatcgt ctggaacagc gtctggcaga ggaaaaaatc actgaagtga
    15841 tcctcgccac caatcccacg gttgaaggtg aagctaccgc taactacatt gccgagcttt
    15901 gcgcgcaata tgacgtggaa gctagccgaa tcgctcatgg cgtaccggtt ggcggcgaac
    15961 tggaaatggt cgatggcacc acgctgtcac actcccttgc cgggcgtcat aagattcgtt
    16021 tttaagcaaa cgagagcagg atcacctgct ctcgcttgaa attattctcc cttgtcccca
    16081 tctctccctc atcctgtttt taaccttaaa atggcattat tgaggtagac ctacatgaaa
    16141 ggacaagaaa ctcgtggttt tcagtcagaa gtgaaacagc ttctgcacct gatgatccat
    16201 tctctctatt ccaataaaga aatcttcctg cgtgagctta tctctaacgc ctccgatgcg
    16261 gcggacaagc tgcgtttccg tgcgctatct aacccggacc tgtacgaagg tgatggcgaa
    16321 ctgcgcgttc gtgtctcttt cgataaagac aagcgcacgc tgaccatctc cgataacggc
    16381 gtggggatga cccgcgacga agtgattgac catctgggga ctatcgctaa atccggtacc
    16441 aaatcattcc tcgaatccct gggttctgac caggcgaaag acagccagct gatcggtcag
    16501 tttggtgttg gtttctactc tgcgtttatc gtggccgaca aagtgactgt gcgtactcgc
    16561 gcggcaggcg aaaaaccaga aaacggcgtc ttctgggaat cggctggcga aggtgaatat
    16621 accgttgccg acatcaccaa agaagatcgt ggtactgaaa tcaccctgca tctgcgtgaa
    16681 ggcgaagacg agttcctcga tgactggcgc gtgcgttcca tcatcagcaa atactccgac
    16741 catatcgcgc tgccggtaga gatcgaaaaa cgcgaagaga aagacggcga aaccgttatc
    16801 tcctgggaga aaatcaacaa agcgcaggcg ctgtggactc gtaacaagtc ggaaatcacc
    16861 gatgaagagt acaaagagtt ctacaaacac atcgcccacg actttaatga tccgctgacc
    16921 tggagccaca accgcgttga aggtaagcag gagtacacca gcctgctgta catcccgtcc
    16981 caggctccgt gggatatgtg gaaccgtgat cataaacacg gcctgaaact gtacgtccag
    17041 cgtgtgttca tcatggacga cgcagaacag ttcatgccga actatctgcg cttcgtgcgt
    17101 ggtctgattg actccagcga tctgccgctg aacgtttccc gtgaaatcct ccaggacagc
    17161 acggtaacgc gtaacctgcg caatgcgctg accaagcgtg tgctgcaaat gctggaaaaa
    17221 ctggcgaaag acgacgcgga aaaataccag accttctggc aacagtttgg cctggtactg
    17281 aaagaaggtc cggcggaaga tttcgctaac caggaagcga tcgccaaact gctgcgtttt
    17341 gcttctaccc ataccgattc ttctgcgcag accgtatctc tggaagacta cgtttcccgc
    17401 atgaaagaag ggcaggagaa aatctactac atcaccgcag acagctatgc ggcagcgaag
    17461 agcagcccgc acctggaact gctgcgtaag aaaggcatcg aagttctgct gctttccgac
    17521 cgcatcgatg agtggatgat gaactatctg actgagttcg acggtaaacc gttccagtcg
    17581 gtgtctaaag ttgacgagtc gctggaaaaa ctggctgacg aagttgatga gagcgcgaaa
    17641 gaagcggaga aagcactgac tccgttcatc gaccgtgtga aagccttgct cggcgagcgc
    17701 gtgaaagatg tccgtctgac tcaccgtctg accgatacgc cagcgatcgt ttcgaccgac
    17761 gcggacgaaa tgagcactca gatggcgaaa ctgttcgctg cggcgggcca gaaagtgcca
    17821 gaagtgaaat acatcttcga actgaacccg gatcacgtac tggtgaaacg tgcggcagat
    17881 actgaagatg aagcgaagtt cagcgagtgg gtagaactgc tgctggatca ggcgctgctg
    17941 gcagaacgcg gcacgctgga agatccgaac ctgtttattc gtcgtatgaa ccagctgctg
    18001 gtttcctgat gtcatgccgg atgacctttg tgtcatccgg catttttctt ttcatcatct
    18061 gcactttccg caaattatct cgccattaac cgtttcagcc tcaggtgcct ttcttgaggc
    18121 aatcgcctgt tggtggtatc gtttatcgct ttttcaaaaa attcgacaca ttttaagggg
    18181 attttcgcaa tgcgtatcat tctgcttggc gctccgggcg cggggaaagg gactcaggct
    18241 cagttcatca tggagaaata tggtattccg caaatctcca ctggcgatat gctgcgtgct
    18301 gcggtcaaat ctggctccga gctgggtaaa caagcaaaag acattatgga tgctggcaaa
    18361 ctggtcaccg acgaactggt gatcgcgctg gttaaagagc gcattgctca ggaagactgc
    18421 cgtaatggtt tcctgttgga cggcttcccg cgtaccattc cgcaggcaga cgcgatgaaa
    18481 gaagcgggca tcaatgttga ttacgttctg gaattcgacg taccggacga actgattgtt
    18541 gatcgtatcg taggccgccg cgttcatgcg ccgtctggtc gtgtttatca cgttaaattc
    18601 aatccgccga aagtagaagg caaagacgac gttaccggtg aagaactgac tacccgtaaa
    18661 gacgatcagg aagaaaccgt acgtaaacgt ctggttgaat accatcagat gactgcaccg
    18721 ctgatcggct actactccaa agaagcggaa gcgggtaaca ccaaatacgc gaaagttgac
    18781 ggcaccaagc cggttgctga agttcgcgct gatctggaaa aaatcctcgg ctaatcaagc
    18841 cgcagcagat ctgctttcgg gcaggtctgc tcatccacct caggcaatac ctctcacagc
    18901 aattagttct tcttcctcac ttttccgcta caattatcaa caagttgaat cgataagagg
    18961 cggtaatgcg tcagactaaa accggtatcc tgctggcaaa cctgggtacg cccgatgccc
    19021 ccacacctga agcggtaaaa cgctatctga aacaattttt aagcgacaga cgcgtggttg
    19081 atacctcacg gttgttatgg tggccattgc tgcgcggcgt tattttgccg ctgcgctcgc
    19141 cgcgtgtggc gaagctgtat gcctctgtct ggatggaagg tggctcgccg ctgatggttt
    19201 acagccgcca gcaacagcag gcgctggcac aacgtttacc ggagacgccc gtagcgctgg
    19261 gaatgagcta cggctcgcca tcactggaaa gcgccgtcga tgaactcctg gcagagcatg
    19321 tagatcatat tgtggtgctg ccgctttatc cgcaattctc ctgttctacg gtcggtgcgg
    19381 tatgggatga actggcacgc attctggcgc gcaaacgtag cattccgggg atatcgttta
    19441 ttcgtgatta cgccgataac cacgattaca ttaatgcact ggcgaacagc gtacgcgctt
    19501 cttttgccaa acatggcgaa ccggatctgc tgctgctctc ttatcatggc attccccagc
    19561 gttatgcaga tgaaggcgat gattacccgc aacgttgccg cacaacgact cgcgaactgg
    19621 cttccgcact ggggatggca ccggaaaaag tgatgatgac ctttcagtcg cgctttggcc
    19681 gggaaccctg gctgatgcct tataccgacg aaacgctgaa aatgctcgga gaaaaaggcg
    19741 taggtcatat acaggtgatg tgcccgggct ttgctgcgga ttgtctggag acgctggaag
    19801 agattgccga gcaaaaccgt gaggtcttcc tcggtgccgg cgggaaaaaa tatgaatata
    19861 ttccagcgct taatgccacg ccagaatata tcgaaatgat ggctaatctt gttgccgcgt
    19921 atcgctaaag ctgagcggta aagaactgag cgccgtcgcg aagagcctcg tcggcggttt
    19981 tcatcattcg tgaataatgc aaaaaggcat gcagcgtacc tgggtagagt ttgaactcac
    20041 agggctgctg atgcgccgct aacgtctggt aaagcagacg gctgtcatcc agcagcggat
    20101 cgaactccgc cccggcaata aaacagggcg gaacttcgcg agtgagatca ttattaaaca
    20161 gacagtaata cggtgactcg cggtccgcgt cgttgcttaa atacgcctct tcatacatct
    20221 gcaaatcctg ttgcgttaag ccatcccaga caccgcccaa cagacgacga gtcacggaat
    20281 cccgtaatcc gtaaagccca taccacagca aaacacccgc aattttaccg caatcgatct
    20341 gtttatcacg caaccacaac gcactggcga gcgccagcat ggcacctgcg gaatcaccgg
    20401 caaagccaat gcgggacata ttgatttgat aatcctccgc ctgctggtgg aaataacaac
    20461 aagcagccac aatttcctct atcgcttgcg gaaaacgcgc ttcaggtgaa agggggtaat
    20521 caataccaat caccgtacat tggctgtagc ttgccagcag gcgcatgatg cgatcgtggg
    20581 tatcgagatt gccgagaata aaaccgcctc catgcaaata aaatagcgtc gccgggctat
    20641 ctggctgcgg acaaaagaga cgtgtttcca cctgcccata ttttgttgga accatgtaag
    20701 ctctggttgc catttctgga gcgcccgcat tccagaatcg gcgctcaagc gtgtaatact
    20761 gtcgttgctc agcaatcgtt cccgttgcgg gccaggacgg taaatccggc tgaagagtat
    20821 tcacaacggt cttcatttca gcagaaataa ggtccagaac aggtagtttg ttttccggct
    20881 tcatacaaaa actcctttca aattacgtca ttgtaaggaa ccactgccat gaaaatgcga
    20941 tcccgcctgc tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga ttgtgttaac
    21001 attcgccgct cagttaacca cccgtaaaaa caaccatgaa atttcccggt aaacgtaaat
    21061 ccaaacatta cttccctgta aatgcacgcg atccgctgct tcagcaattc cagccagaaa
    21121 acgaaaccag cgccgcctgg gtcgtaggta ttgaccagac gctggtcgat attgaagcga
    21181 aagtggatga tgaattcatt gagcgttatg gattaagcgc cgggcattca ctggtgattg
    21241 aggatgacgt agccgaagcg ctttatcagg agctaaaaca gaaaaacctg attacccatc
    21301 agtttgcggg tggcaccatt ggtaacacca tgcacaacta ctcggtgctc gcggacgacc
    21361 gttcggtgct gctgggcgtc atgtgcagca atattgaaat tggcagttat gcctatcgtt
    21421 acctgtgtaa cacttccagc cgtaccgatc ttaactatct acaaggcgtg gatggcccga
    21481 ttggtcgttg ctttacgctg attggcgagt ccggggaacg tacctttgct atcagtccag
    21541 gccacatgaa ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc ggagcctcgg
    21601 cactggttct cacctcttat ctggtgcgtt gcaaaccggg tgaacccatg ccggaagcaa
    21661 ccatgaaagc cattgagtac gcgaagaaat ataacgtacc ggtggtgctg acgctgggca
    21721 ccaagtttgt cattgccgag aatccgcagt ggtggcagca attcctcaaa gatcacgtct
    21781 ctatccttgc gatgaacgaa gatgaagccg aagcgttgac cggagaaagc gatccgttgt
    21841 tggcatctga caaggcgcta gactgggtag atctggtgct gtgcaccgcc gggccaatcg
    21901 gcttgtatat ggcgggcttt accgaagacg aagcgaaacg taaaacccag catccgctgc
    21961 tgccgggcgc tatagcggaa ttcaaccagt atgagtttag ccgcgccatg cgccacaagg
    22021 attgccagaa tccgctgcgt gtatattcgc acattgcgcc gtacatgggc gggccggaaa
    22081 aaatcatgaa cactaatgga gcgggggatg gcgcattggc agcgttgctg catgacatta
    22141 ccgccaacag ctaccatcgt agcaacgtac caaactccag caaacataaa ttcacctggt
    22201 taacttattc atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc tatcaggtac
    22261 tgaaccagca ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac agcctggaag
    22321 agtcttactg ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc gttcacgccg
    22381 catctggcat ttggctctcg atgcctgatg cgacgctggc gcgtcttatc atgcctacat
    22441 atttttcata ttttacatcc ggcaaccacc gtttaccccg tcaccacctc acccgccggt
    22501 ggcgtttcca gcagttccag catggtacgg gcgatttcac gctcgcccat cactacctga
    22561 ttcgcaccac gttcggtgat atacgccact tcatcgtcat aatgggcgcg ggcaataatc
    22621 tcaatatccg gatttttcgc gcgggcagat gccacaatct cacccgcttc ataaccgttg
    22681 ggaatcgtca ggatcagcca ttttgcacat tccagatgcg ccagttgcat aatttcttcg
    22741 ttcgccgcat tgcccaatac tgcgcggacc ccgcgctctc gcagctcatc aacacgggtt
    22801 cgtgacgtct caatcaccac cagcggaata tcagaggcga gcaatttctc ccccagcagg
    22861 ctgcctacac gaccgtaacc caccagtagc gcatggttgc aaatatccac tgggatctgc
    22921 ttctcttctt cgattgcctc ttccagcgtc tgctcttcca gcgtttcggt cttcgccaga
    22981 tatttctcca gtagtgcgaa cagtaccggg ttgagcataa tcgacaggat cgcccctgcc
    23041 agtaccaggt tttgtccggc ctgcggcagt aaattcaatg ccattcccag tcccgccagg
    23101 ataaacgcga actcaccaat ctgcgccagg ctggcggcga tggttaatgc cgtacgttgg
    23161 gagtgaccaa acagtcgcac caggaaaaat gcggctaacg acttaccaaa cagaataatc
    23221 gccagcgtcg ccagcactgc cagcggttgc tgaatcagaa ttaacggatc aaacaacatc
    23281 ccgacggaga caaaaaacag caccgcaaac gcgtcgcgca atggcagcgt atcgtgggcg
    23341 gcacggtgac tcagttcaga ctcgttcagt accatcccgg caaagaacgc accgagtgca
    23401 aaggagacat caaacagctc taccgcacca aaggcaatcc ctaacgccag cgccagcacc
    23461 gacagggtaa acagctcgcg agaaccggtt gccgcgctgc gtgccataat ccacggcacc
    23521 agacggcgac ctaccagcat cataatggcg ataaatgcga tcactttgcc gatggtgatc
    23581 cccatatcga ctgcaagagt ggcaaagccc acatcgccct gttccatcat tcctgcaact
    23641 gcgggcagca acaccagcgt cagaaccatt accaggtctt ccacaatcaa ccaaccgatg
    23701 gcgatttgcc cacgctgact gtcaattaat tgccgttctt caagtgcgcg cagtaacacc
    23761 acggtactgg cggtggaaag acataaaccg aacacgatac cggtcattaa cgaccagccc
    23821 agcacggcag agagcgccat acccagcagc gtcgccacgg ctatctgggc gatcgcaccg
    23881 ggaatggcga tggcctttac cgccatcaaa tccttcagcg aaaagtgcaa accgacgcca
    23941 aacatcaaca gaatgacgcc cagttcagcc agttccggcg caagcttggt atcggcaaca
    24001 aagcccggag tgaatggtcc tgccagcaca cccgctaaca gatatcccac cagaggagaa
    24061 atacgtagtt tattggccag catgccgagg ataaaggcga gcacaaggcc gccaacaatg
    24121 gtggtgataa gcggggtggc gtgatgcatt ccgtctcctt ttcctggtgg ttattgtcca
    24181 tttttggccg ggaaaaccaa aattacaggt aatagtttat gacaatttca ttgatgatgt
    24241 tcatgaataa ttattgaatt ttgcagaaaa atggaattag ctgcaaaaaa agcacggatc
    24301 ggaaaacaga aggcggttta acagaggaaa ggcttatggc gactgtaaaa cgatgcagcc
    24361 aaagtttgcc tttggctgca atgaaatcag tctttatgcc ggttatcagg caggaat
//
LOCUS       DAJTSL010000052        24086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTSL010000052 DAJTSL010000000
VERSION     DAJTSL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.052"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            324..551
                     /gene="feoA"
                     /locus_tag="OO433_001849"
     CDS             324..551
                     /gene="feoA"
                     /locus_tag="OO433_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6050352.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            568..2889
                     /gene="feoB"
                     /locus_tag="OO433_001850"
     CDS             568..2889
                     /gene="feoB"
                     /locus_tag="OO433_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6050353.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2889..3125
                     /gene="feoC"
                     /locus_tag="OO433_001851"
     CDS             2889..3125
                     /gene="feoC"
                     /locus_tag="OO433_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6050354.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3328..4206
                     /gene="rpnA"
                     /locus_tag="OO433_001852"
     CDS             3328..4206
                     /gene="rpnA"
                     /locus_tag="OO433_001852"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6050355.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMGCLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(4235..5005)
                     /gene="bioH"
                     /locus_tag="OO433_001853"
     CDS             complement(4235..5005)
                     /gene="bioH"
                     /locus_tag="OO433_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6050356.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            5043..5726
                     /gene="gntX"
                     /locus_tag="OO433_001854"
     CDS             5043..5726
                     /gene="gntX"
                     /locus_tag="OO433_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6050357.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5785..6360
                     /gene="nfuA"
                     /locus_tag="OO433_001855"
     CDS             5785..6360
                     /gene="nfuA"
                     /locus_tag="OO433_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6050358.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6721..8037
                     /gene="gntT"
                     /locus_tag="OO433_001856"
     CDS             6721..8037
                     /gene="gntT"
                     /locus_tag="OO433_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6050359.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8082..10166)
                     /gene="malQ"
                     /locus_tag="OO433_001857"
     CDS             complement(8082..10166)
                     /gene="malQ"
                     /locus_tag="OO433_001857"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6050360.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKQ"
     gene            complement(10176..12569)
                     /gene="malP"
                     /locus_tag="OO433_001858"
     CDS             complement(10176..12569)
                     /gene="malP"
                     /locus_tag="OO433_001858"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6050361.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQEVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATIGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKLLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLQDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEISEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13181..15911
                     /gene="malT"
                     /locus_tag="OO433_001859"
                     /pseudo
     CDS             13181..15911
                     /gene="malT"
                     /locus_tag="OO433_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(15954..16970)
                     /gene="rtcA"
                     /locus_tag="OO433_001860"
     CDS             complement(15954..16970)
                     /gene="rtcA"
                     /locus_tag="OO433_001860"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6050362.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGAVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(16974..18200)
                     /gene="rtcB"
                     /locus_tag="OO433_001861"
     CDS             complement(16974..18200)
                     /gene="rtcB"
                     /locus_tag="OO433_001861"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6050363.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKDSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIKICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMTAQSD
                     LVEVIYTLRQVVCVKG"
     gene            18389..19987
                     /gene="rtcR"
                     /locus_tag="OO433_001862"
     CDS             18389..19987
                     /gene="rtcR"
                     /locus_tag="OO433_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6050364.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWHPTLCLCQQESLVIDRL
                     ELLHDARSCSLFETLKRDIASVSPETEVVSIEIELHNPWDFEEVYACLHDFARGYKFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQTSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFFDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(19969..20727)
                     /locus_tag="OO433_001863"
     CDS             complement(19969..20727)
                     /locus_tag="OO433_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6050365.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(20744..21574)
                     /gene="glpG"
                     /locus_tag="OO433_001864"
     CDS             complement(20744..21574)
                     /gene="glpG"
                     /locus_tag="OO433_001864"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6050366.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIIAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(21619..21945)
                     /gene="glpE"
                     /locus_tag="OO433_001865"
     CDS             complement(21619..21945)
                     /gene="glpE"
                     /locus_tag="OO433_001865"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6050367.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            22135..23640
                     /gene="glpD"
                     /locus_tag="OO433_001866"
     CDS             22135..23640
                     /gene="glpD"
                     /locus_tag="OO433_001866"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6050368.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(23692..>24086)
                     /locus_tag="OO433_001867"
     CDS             complement(23692..>24086)
                     /locus_tag="OO433_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050369.1"
                     /translation="VMAGIGWQFSRRTPPELKNRLHCWLVFSPVYFWLILSNFILYIS
                     PEKSALLGNIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGFALGYYCHWCRKNR
                     NQRKKWGDWVTCLSLAILAQGPLFNYLQ"
BASE COUNT         5659 a         6560 c         6095 g         5772 t
ORIGIN      
        1 accaaactca taatatattc cggcaatatt tatcacttca ttaacaactg aaaccttaat
       61 taaacattag ccagtccggg taattcacta ttcgaattat attttcgctg cgatataacc
      121 ttgagccaca tcaacattga gtcagattat tattcaaacc aacattcgca cacattttaa
      181 gtattgctga tagaaaccat tctcattatc attgtgttat tgattattta atctctcctt
      241 tgttggcaaa tcatctggtc tcatgtcgct gtcaaacgcc ccatgaggta gttatccagt
      301 taatgagaaa caagtaggca cctatgcaat acactccaga tactgcgtgg aaaatcactg
      361 gcttttcccg tgaaatcagc ccggcatatc gccaaaaact gctttctctt ggcatgttac
      421 ctggctcctc ttttaatgtg gtgcgcgtcg ctccactcgg cgaccccatt catatcgaaa
      481 cccgtcgtgt gagcctggta ttacgcaaaa aagatctggc cttattagaa gtggaagcgg
      541 tttcctgtta atacggtgat aacaacaatg aaaaaattaa ccataggctt aattggtaat
      601 ccaaattctg gcaagacaac cttatttaac cagctcactg gcgcacgtca gcgtgtaggt
      661 aactgggctg gcgttaccgt cgaacgtaaa gaagggcaat tctccaccac cgatcatcag
      721 gtcacgctgg tggacctacc cggcacctat tctctgacca ccatctcatc gcagacctcg
      781 ctcgatgagc aaatcgcctg tcactacatt ttgagtggcg acgccgacct gctgattaac
      841 gtggtggatg cgtctaacct tgagcgtaac ctgtacctga cgctacaact gctggaactc
      901 ggcattccct gcattgtggc actgaacatg ctcgacattg ccgagaagca aaatattcgt
      961 attgaaattg atgctctgtc ggcgcgtctg ggctgtccgg tgatcccgct ggtttcaacc
     1021 cgtggtcgcg gtattgaagc gctcaagctg gcgattgatc gctataaagc taacgagaat
     1081 gtggaactgg tgcattacgc acagccgctg ctcaacgaag cagattcact ggcaaaagtg
     1141 atgccttccg acatcccgct gaaacaacgt cgctggctgg gcctgcaaat gctggaaggc
     1201 gatatctaca gccgcgccta cgccggtgaa gcgtcgcagc atctggatgc cgccctcgcc
     1261 cgtctgcgta atgagatgga cgatccggcg ctgcacattg ccgatgcgcg ttaccagtgc
     1321 attgctgcca tctgtgatgt ggtaagcaac accctgacgg cagaacccag ccgtttcacc
     1381 actgcggtag ataaaatcgt gctcaaccgt ttcctcggtc tgccgatttt cctctttgtg
     1441 atgtacctga tgttcctgct ggctatcaac atcggcgggg cgttacagcc gctgtttgac
     1501 gtcggctccg tggcgctatt tgtgcatggt attcaatgga ttggctacac gctccacttc
     1561 ccggactggc tgactatctt cctcgcccag ggcctgggtg gcggcattaa caccgtgctg
     1621 ccactggtgc cgcagattgg catgatgtac ctgttcctct ccttccttga ggactccggg
     1681 tatatggcgc gtgcggcgtt tgtgatggac cgtctgatgc aggcgctggg cttgccgggg
     1741 aaatcctttg tgccgctgat cgtcggtttc ggttgtaacg taccgtcggt aatgggtgca
     1801 cgtacgcttg atgcaccgcg tgaacgtctg atgaccatca tgatggcacc gtttatgtcc
     1861 tgcggcgcgc gtctggctat cttcgcagta ttcgcggctg ccttcttcgg gcagaacggt
     1921 gcgctggcgg tcttctcgct gtatatgctg ggtattgtga tggcggtgct gactggcctg
     1981 atgctcaagt acaccatcat gcgcggtgaa gcgacgccgt ttgtcatgga gttgccggtc
     2041 tatcatgtac cacacgttaa aagcctgatt atccagacct ggcagcgtct gaaaggcttc
     2101 gttctgcgtg ctggtaaagt gatcatcatc gtcagcattt tcctgagcgc tttcaacagc
     2161 ttctcgctga gcgggaaaat cgtcgataac atcaacgact cggcgctggc gtccgtcagc
     2221 cgggtgatca ccccggtctt caagccaatt ggcgtgcatg aagataactg gcaggcaacg
     2281 gttggcctgt ttacaggtgc catggcgaaa gaagtggtag tgggtacgct caacaccctc
     2341 tacaccgcag aaaatattca ggacgaagag ttcaatccgg cagagtttaa cctcggtgaa
     2401 gagctgttca gtgcgataga tgaaacctgg cagagcctga aagacacctt cagccttagc
     2461 gtactgatga accccattga agccagcaaa ggcaacggcg aaatgggtac cggggcgatg
     2521 ggcgtgatgg atcagaaatt cggtagcgca gcagcagctt acagctacct gattttcgtc
     2581 ctgctgtatg taccatgtat ctcggtgatg ggagccatcg cccgtgaatc aagccgtggc
     2641 tggatgggct tctccatcct gtgggggctg aatatcgctt actcgctggc aacattgttc
     2701 tatcaggtcg ccagctacag tcagcaccca acttacagcc tggtgtgcat tctggcggtt
     2761 atcctgttta acatcgtggt tatcggtctg ctgcgccgcg cgcgtagccg ggtggatatc
     2821 gaactgctgg caacccgcaa gtcggtaagc agttgctgcg cagccagcac caccggtgat
     2881 tgccattaat ggcttcactt attcaggtgc gcgatttgct ggcgttacgg ggccgtatgg
     2941 aagcggccca gataagccag acattgaaca ctccacagcc aatgattaac gccatgctgc
     3001 aacaactgga aagtatgggc aaagccgtgc ggattcagga agaacctgac ggctgcctct
     3061 ctggcagttg taaaagctgc ccggaaggaa aagcctgtct gcgcgagtgg tgggcgcttc
     3121 gttaacctta ctccatcgcc tgttttggaa agcccggtat gcgtctgcat ccgggctttt
     3181 ttgcgtgcgg ctttccataa aaatgcaact cttgcagcac ggcgtaagtt cctttgaaag
     3241 catctcgcag ggatgaaaac tcgctaatac acaggtgtgg agtggcgcgt agagtcgcgg
     3301 cattcaaaca acaggtgaag gaacgccatg agcaaaaagc agagttccac cccacacgat
     3361 gcgctgttca aactcttttt acgccaaccg gacacggctc gtgattttct tgcgtttcat
     3421 ttaccggcac ccattcacgc gctttgtgat atgaaaaccc tcaagctgga gtcgagcagc
     3481 tttattgatg acgatctgcg tgaaagctat tccgatgtgc tgtggtcggt gaaaacggaa
     3541 caaggaccag gatacatcta ttgtctgatt gaacatcaaa gcacctcaaa caaactgatc
     3601 gcatttcgca tgatgcgtta cgctattgcc gcaatgcaaa atcaccttga tgctggatac
     3661 aaaacgttgc cgatggtggt gccattgttg ttttaccacg gtattgaaag cccctatccc
     3721 tattcgctgt gttggctgga ttgtttcgcc gatcccaaac tggcaaggca gctttatgcc
     3781 tccgcatttc cgctgattga tgtcaccgtc atgcctgatg atgaaatcat gcagcaccga
     3841 cgtatggcgc tgctggagtt aattcaaaaa catattcgtc aacgcgacct gatggggcta
     3901 gtagagcaaa tgggctgctt attaagtagt ggatacgcta atgacagaca aatcaaaggg
     3961 ctgtttaatt acatactgca aaccggcgac gcggtacgtt ttaacgattt tatcgacggc
     4021 gttgccgaac gttcaccgaa acacaaggag agtttaatga caattgcgga aagattgcgg
     4081 caggaggggg aacaatccaa agccctgcat atagccaaaa taatgcttga atccggagtc
     4141 cctcttacag acatcatgcg ctttaccggg gtgtcagaag aagagttggc tgcggcgagc
     4201 cagtaaattt ctgtctcgcc atttcaaaag ttgcctacac cctctgcttc aacgccacca
     4261 gcaggtgaca aaactcgacc ggatgcgaaa taaacggcgc atgggccgct ttggcgaaga
     4321 tatatgattt gctgcgaggc cagagtttat ccagcatcgg caccactttg cgcggcacca
     4381 gaccatcgag atagccatac aatcgcaaaa acggcatggg cacgttttgc agcggctgac
     4441 ggagatcaac cgttttcagg atttccagcc cgccattaag cacgtcaacc tccggcatcg
     4501 gtaacgccag aacggttttc ttcaacgccc gcgcatcctg gcgcgccgtt tcagtcccca
     4561 tggtttgtaa cgccaggaac cgctccactg tacgctgaaa atcatcactg agttgctgct
     4621 gaaatcccgc cagcacgtcc ggttttatcc ccggccaatc gtcacgagca ctaaaacaag
     4681 gtgacgacgc cacggtgacc agcgcctgaa cacgctcggg atgggttaac gcaatctggc
     4741 ttgccaccag cccgcccaga ctccagccta accaaatggc tttatcaggt gcctgttgca
     4801 gcacggcttc ggccatatca gcaagtgaca gcgcaccaaa tccctggctg cgcccgaagc
     4861 cgggcagatc aacaaggtgc agcgtaaaat gcgagctaag ttcctcgtca atgcaacgcc
     4921 acacttcggc attcagtccc catccgtgca gcagcacaag atgaacattc ccctgacctt
     4981 tggtctgcca ccagatgtta ttcatccgct attgttctct tttgacttac aaggatgaac
     5041 atatgctaac agtaccggga ttatgctggc tatgccgaat gccgctggcg ttaggtcatt
     5101 gggggatttg ttcggtctgc tcacgcgccg cccgtacaga taaaacgtta tgcccacaat
     5161 gtggattacc cgccacacac tcacatcttc cctgcggtcg ctgcctgcaa aaaccaccgc
     5221 cctggcaaag actggtcacg gttgccgact atgcgccgcc gttaagtccg cttatccacc
     5281 agcttaaatt ttcccggcgc agtgaaatcg ccagcgccct gtcacgtctg ttactattgg
     5341 aagtcttaca cgctcgtcgc accaccgggt tgcaattgcc ggatcgcatc atcagcgttc
     5401 cgttatggca gcggcgtcac tggcgtcggg gatttaatca gagcgatttg ctgtgtcagc
     5461 cgttatcacg ctggttgcac tgccgatggg atagcgaagc cgtcacacga acacgggcca
     5521 ctgcgaccca gcattttctc agtgcccggc tacgcaagcg caacctgaaa aatgcctttc
     5581 gtcttgaatt gcccgtgcaa ggtcgccata tggtgattgt ggatgatgtc gttaccaccg
     5641 gaagtaccgt cgcagagatt gcgcagttgc ttttacgcaa tggtgcggcg actgtccagg
     5701 tctggtgcct ttgtcgaacc ttgtagagcc tcgatgatag gcgtattata accaactaaa
     5761 atagtcaact attaggccat tactatgatc cgtatttccg atgctgcaca agcgcacttt
     5821 gccaaactgc tggcaaacca ggaagaaggg acacaaatcc gcgtatttgt gattaaccct
     5881 ggcacgccta acgctgaatg tggtgtttct tattgtccgc cggatgccgt ggaagccacc
     5941 gacactgccc tgaaatttga cctgctgacc gcgtatgttg atgagttaag cgctccttac
     6001 ctggaagatg cagagatcga ttttgttacc gaccagttgg gttctcagct aacactgaaa
     6061 gcgcctaacg ccaaaatgcg taaagtggca gacgatgcac cactgatgga gcgcgtggag
     6121 tatatgctgc agtcgcagat caacccacag cttgctggtc acggtggccg cgtttcactg
     6181 atggaaatca ccgaagacgg ttacgccatt ctgcaatttg gcggcggctg taacggttgt
     6241 tccatggtcg atgtgacgct gaaagaaggg atcgagaagc agctgctaaa cgaattcccg
     6301 gagctgaaag gtgtgcgcga tctcaccgaa caccagcgcg gcgaacactc ctactactaa
     6361 gttatcccct cttttgtaga ttgcccgatg cgacgctaaa gcgtcatttc gggcctacgt
     6421 tgaacatgtg ccagacgtgg gcgcagcatt gctttggcaa atccctccct tcgtttctcc
     6481 gcgataatat gaccaacctc tcataattta aatttactcc gctctggtga ttctcaaacg
     6541 ccagatgtta ccggtatcat tcacatgggt accaaacata ctcctgacat ctgactacaa
     6601 taattagttt tagtgggtat cagtcgtggt gccgcaatat ctctgttccc gattgggata
     6661 attagagttt gtcgtcagaa aattgacgtt acccgtaaca aatgaaaggc caggtaaatc
     6721 atgccattag tcattgttgc tatcggtgta atcttgttgt tgctcctgat gatccgtttc
     6781 aaaatgaacg gcttcatcgc tctcgtcctc gtggcgcttg ctgttggatt aatgcaaggg
     6841 atgccgctgg ataaagttat tggctccatc aaagccggtg tcggcgggac gctcggtagc
     6901 cttgccctga tcatgggttt tggcgcgatg ctgggcaaaa tgctggcaga ctgcggtggc
     6961 gcacaacgta tcgccaccac cctgattgcc aaatttggta aaaaacacat ccagtgggcg
     7021 gtggtactga ccggttttac cgttggtttt gccctgttct atgaagtggg ctttgtgctg
     7081 atgctgccgc tggtgtttac catcgcggct tctgcgaata ttccactgct gtatgttggt
     7141 gtaccaatgg cagctgcact gtctgtgacc cacggcttcc tgccaccgca tccgggtccg
     7201 actgcgattg ccaccatttt caatgccgac atgggtaaaa ccctgctgta cggtactatt
     7261 ctggcaatcc cgaccgtgat tctcgccggt ccggtttacg ctcgcgtgtt gaaaggtatc
     7321 gataagccga tccctgaagg tctgtacagc gcgaaaacct tcagcgaaga agagatgccg
     7381 agctttggcg tcagcgtctg gacctctctg gtgccggtag tgctgatggc gatgcgtgcg
     7441 attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc cggtagcgga gttcctcggt
     7501 gacccggtaa tggctacgct gattgccgtg ctgattgcga tgttcacctt tggtctgaac
     7561 cgtggtcgtt caatggatca gattaacgac acgctggttt cttccatcaa aatcattgcg
     7621 atgatgctgt tgatcatcgg tggtggcggt gcgttcaagc aggtgctggt agacagcggc
     7681 gtggacaaat acattgcttc catgatgcac gaaaccaaca tttctccgct gctgatggcc
     7741 tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg caaccgttgc ggcaatcact
     7801 gcgggtggta tcgcggcacc gctgattgca acgacgggtg ttagcccgga gctgatggtt
     7861 attgcggtgg gttccggtag tgtgattttc tctcatgtga acgatccggg cttctggctg
     7921 ttcaaagagt actttaacct gactatcggc gagaccatca aatcctggtc gatgctggaa
     7981 accattatct cggtgtgcgg tctggtaggc tgtctgctgc tgaatatggt gatttgataa
     8041 cactgactgc cggatgcatc gcatccggca tcaaccgcac tctactgctt cttcgctgca
     8101 gctctgcgcc gtctgtccaa atccttcagc aacttattca cgccatcatc ggcaaacatc
     8161 gtctcaagcg ttgcggaaag cttgcgtcgc cagtttttat actggtaact ggtgccagga
     8221 atattcaccg gttcggccat atccagccag tcttccggct gtagtcctaa cagagcactg
     8281 ttactgtcgg caatgtagcg ctgcaaacca cgattaagcg tcggcgtcat cgacatcaac
     8341 gatgccttat gcccggcacg ttttggcaga caaccatatt tatgcagtgc atccagcagc
     8401 ccttgcttcg ccagttcgcg atcctgatac agaccgcgca gtaccacttc atccggatac
     8461 agccccaggg ttttgcccaa cgttagatcc ccgctctccc agtaaccgcg cagcgtaggc
     8521 aggtcatgtg tcgccgcaac cgccatcgac tgctccggat acgctttcgg tgcacggaac
     8581 gtcttctcgt ggtcgttttc gaaatagagc actttgtagg agtacacacc gctgctgcgc
     8641 agcttaccga caatctctac cggtacggta ccgagatctt caccaatcac catacagcga
     8701 tgacgtttac tttcgagtgc cagaatcgag agcagatcat ccaccggata gtgaacatac
     8761 gcgccctgat ctgccgtctc gccatacggt atccaccaca aacgcagcat cgacatcaca
     8821 tggtcaattc gtaatgcgcc gcagttttgc atattggcac gcaacagctc gataaacggt
     8881 tcataggcac gcgcggtgat gatatgcggg tccattggcg gtaatcccca gttctgcccc
     8941 aacgggccga ggatatccgg cggcgcgcca accgacgctt tcaggcaata tagttcacgg
     9001 tcacaccagg tttccgcccc accttccgct acgccaaccg ccagatcacg atacaagcca
     9061 atcggcattt catagccctg gcttatctcc cagcaggcgg caaactggct gtaagccagc
     9121 cactgcaacc agagataaaa atcgacgtca tcacgatgtt cttcgcagaa ctgacgcact
     9181 tctggtgaat ccacgttctg atacatctct ggccatgcag gccagcccca gcgcatttcg
     9241 tcctctttca cttgctgggc atgtagcgca tcaaaggctg cctgccagaa caggctgtcg
     9301 ccctgctctg caacaaactg gcgaaacgcg gtcatctgct catcatcacg ttgcgcgaaa
     9361 cctttccacg ccattcgtaa tgctgtcatt tttagggcgg taaccgtgga gtaatcgacc
     9421 cagtcggcat cgcgcgcctg ttgcagcgtc tgttgcgtgg tcggcaactg ccaccaggcc
     9481 tgagcctctt cgctaagatg gaaatcttca acggcgttaa cgtcgatata aatcacattc
     9541 agccaacggc gagaagacgg actgtatggg ctggcactct ccggatttgc cggatagagc
     9601 gcatgaatcg ggttcaggcc gatgaacgac ccgccacgtt ttgccacatc caccagcatc
     9661 gctttgagat cgccaaaatc cccaataccc cagttttttt ccgatcgcag cgtataaagc
     9721 tgaacgcagg caccccacag cttttgttta ttcagcaacg cctgcggttc gtaacagcgt
     9781 ttcggggcga caatcacccg gcaatgcgcg cgctggtcgt cctgggtgag tgtcagcgtg
     9841 tgataacctt ccggcagctt cgtcggtaga ttgaacgctt tgccccccgt tacatggcct
     9901 ttgtactgtg ttccttcttc ggtggtcagc agccagctat attcgccgct gccctccacc
     9961 accatcggca tttttttgcc gctggtataa accatgacat tcgggactgg cgttaccgcc
    10021 actttcgtgg cggtacgttg atgcatcgcg tcaagcaaac gccgtttggt ttcggcgcta
    10081 atcgactgcg gtttaccgtg ggcattgatg taattggggc taatccccgc cgccagcgcg
    10141 gcattatcca gacgtttgct ttccatcgag cttccttagc gttttgcctg ccagatacga
    10201 gcctgataat cgcgaataga gcgatccgag ctaaacatac cgcagcgggc ggtattgagg
    10261 atcgccgcgc gagtccaggc ctcctggtcg cggtacagca catccacctg cttttgtgcc
    10321 tcaacatagg ctgcgaaatc cgccatcacc agatacggat cgccgccttg tttgccgata
    10381 ctgtgcagca tctggtcgaa ggcatgctta tcgccgtcgc tgtatttacc gctttccagc
    10441 tctttcaata ctgcgtccag caccttatct ttcttccgcc atttcaccgg gtcgtagcct
    10501 ttggccagaa ttgccttcac ttgttccacg gtatgaccaa aaataaagat attttcttca
    10561 ccgactttct cggagatttc aacgttcgca ccatccagcg taccgacagt aagcgcacca
    10621 ttgagcgcca gtttcatatt gccggtaccg gaagcttctt tacctgcagt cgaaatttgt
    10681 tcggagatat ccgccgccgg gatcagtttt tccgccgccg aaacgcaata atcctgcagg
    10741 aacaccacct tcaacttatc gccaaccagt ggatcgttgt tgatcacgtc agccactttg
    10801 ttgatcgcaa agataatatt cttcgccagg tagtagcccg gtgccgcttt cgcgccgaag
    10861 aggaagacgc gcggtacgcg atcagcctgc gggttttcac gaatttcttt gtacaacgcc
    10921 agaatatgca gcagattcag gtgctggcgt ttgtactcat gcagacgttt aatctgaata
    10981 tcgaaaatcg cctgtggatt gatctcaata ccggtacgaa ctttcacaaa ctccgccaga
    11041 cggactttat tcgcctgctt gatctcgcga tattgctgac ggaatttcgc atcatcagcg
    11101 aatttttcca gattgatcag ctgatcgaga tcgttagccc actctttttg cagtaattta
    11161 tccaacagag ccgccagtgc agggttgcac tgtttgatcc agcgacgtgg ggtaataccg
    11221 ttggtgacgt tatggaattt gttcggccat agctggtgat attccgggaa cagatctttc
    11281 accaccagat ccgagtgcag cgccgcaaca ccgttcaccg cgaaaccgcc aaccacacac
    11341 aggttcgcca tatgcacttg tttatcgtgc accaccgcca gtttggccca cactttttca
    11401 tcgcccggcc aggttttctc caccagcgtt ttaaagcgag tattaatttc gttaataatc
    11461 tgcatatggc gcggcagtaa gcctttcacc agtttcacat cccagcgttc cagcgcttct
    11521 ggcatcaggg tatggttggt gtaagcgaaa gttttgctgg taatggccca ggcgtcatcc
    11581 cagctcatct ggtgctcatc gatcagcacg cgcagcagtt ctggaatcgc gatagttggg
    11641 tgggtatcgt tcagctgaat aacttcataa tccgccagtt cgtgcagttt acgccccgcc
    11701 agatgatggc gacgcaaaat atccgctacc gaacaggcac actggaagta ctgctgcatc
    11761 aagcgcagct ttttaccggc agtatggttg tcgtttggat agagaacttt ggtcagtttt
    11821 tccgcattga tgccctgctg ttcggcacgc aggaaatcac cgtcgttaaa tttagtcaga
    11881 tcaaacggat gcgcgtgcgt cgcctgccac agacgcagcg gctgcgccac gccattacga
    11941 tagccgacaa cggggagatc ccacgcttga ccggtaatgg taaactccgg ctcccagcgt
    12001 ccgtctttcg tcactttacc gccaatccct acctgcacat ccagtgcttc gttgtggcgg
    12061 aaccacgggt agttactacg atgccagtca tccggcgctt caacctgttt gccatcgaca
    12121 aaagactggc ggaacaaacc atattgatag ttcagaccgt aacccgtcgc agactgaccg
    12181 atagttgcca ttgagtcgag gaagcacgcc gccagacgtc ccagaccacc gttacccagc
    12241 gccgggtcga tctcttcttc cagcaggtcc gtcagattga tgtcataagc cttcaacgaa
    12301 tcctgtactt cctgatacca gccgagattc aacaggttgt tgcccgtcag gcgaccaatc
    12361 aaaaactcca ttgagatgta gttaacatgt cgctgattcg ccaccggctt ggcgaatggc
    12421 tgagcacgca gcatttcggc cagtgcttca ctcactgcta gccaccactg gcgaggagtc
    12481 atttcagccg cagaatttaa gccataacgc tgccactgac gtgaaagcgc ttcctgaaat
    12541 tgcttatcgt taaaaatagg ttgtgacata ggagttccac ttttcttaga ttttcaacac
    12601 aacgttatcg ctagtttgcc aggctcgatg ttgaccttcc tcatcctgcg ggggattagg
    12661 cagggaggag ttgcggggat gagcaaggaa atgtgatctc aaccacttaa agctagtgca
    12721 aaccacagga ttagcagcaa atcaatgcaa tacagcgcag aaaatctgta tctaagtgca
    12781 aaaaatggcc gttgcgtatt ttcaaaaagc ggaaggtaac tctataaatt aagtaaagga
    12841 gtgaaacagt ttcacaagta aaatatccag tgtgctccat ctcattctta atagatttat
    12901 taagatcatc tttttagatg gcactttcat caggaatgaa gaagaaaccc ttgcttaaat
    12961 gaatctgatg aacataaggg aaaccagtat tcacgctgga tcagcgtcgt tttaggtgag
    13021 ttgttaataa agatttggaa ttgtgacaca gtgcaaattc agacacataa aaaaacgtca
    13081 tcgcttgcat tagaaaggtt tctggccgac cttataacca ttaattacga agcgcaaaaa
    13141 aaataatatt tcctcatttt ccacagtgaa gtgattaact atgctgattc cgtcaaaact
    13201 aagtcgtccg gttcgactcg accataccgt ggttcgtgag cgcctgctgg ctaaactttc
    13261 cggcgcgaac aacttccggc tggcgctgat cacgagtcct gcgggctacg gaaagaccac
    13321 cctcatttcc cagtgggcgg caggcaaaaa cgatatcggc tggtactcgc tggatgaagg
    13381 tgataaccag caagagcgtt tcgccagcta tctcattgcc accgtgcagc aggcaaccaa
    13441 cggtcactgt gcgatatgtg agacgatggc gcaaaaacgg caatatgcca gcctgacgtc
    13501 actcttcgcc cagcttttca ttgagctggc ggaatggcat agcccacttt atctggtcat
    13561 cgatgactat catctgatca ctaatccagt gatccacgag tcaatgcgct tctttattcg
    13621 ccatcaacca gaaaatctca ccctggtggt gttgtcacgc aaccttccgc aactgggcat
    13681 tgccaatctg cgtgttcgtg accaactgct ggaaattggc agtcagcaac tggcatttac
    13741 ccatcaggaa gcgaagcagt tttttgattg ccgtctgtca tcgccgattg aagccgcaga
    13801 aagcagtcgg atttgcgatg acgtttccgg ttgggcgacg gcactacagc taatcgccct
    13861 ctccgcccgg cagaataccc actcagccca taagtcggca cgccgcctgg cgggaatcaa
    13921 tgccagccat ctttcggatt atctggtcga tgaggttttg gataacgtcg atctcgcaac
    13981 gcgccatttt ctgttgaaaa gcgccatttt gcgctcaatg aacgatgcgc tcatcacccg
    14041 tgtgaccggc gaagaaaacg ggcaaatgcg tctcgaagag attgagcgtc aggggctgtt
    14101 tttacagcgg atggatgata ccggcgagtg gttctgctat cacccgctgt ttggcaactt
    14161 cctgcgccag cgctgccagt gggaactggc ggcggagctg ccagtgggaa ctggcggcgg
    14221 agctgccgga aatccaccgt gccgccgcag aaagctggat ggcccaggga tttcccagcg
    14281 aagcgattca tcatgcgctg gcggcaggcg atgcgctgat gctgcgcgat attctgctta
    14341 atcacgcctg gagtttgttc aaccatagcg aactgtcgct gctggaagag tcgcttaagg
    14401 ccctgccgtg ggacagtttg ctggaaaatc cgcagttagt gttattgcag gcgtggctaa
    14461 tgcaaagcca acatcgctat ggcgaagtta acaccctgct agcccgtgct gaacatgaaa
    14521 tcaaggacat cagagaaggc accatgcacg cagaatttaa cgctctgcgc gcccaggtgg
    14581 cgattaacga tggtaatccg gatgaagcgg aacggctggc aaaactggca ctggaagagc
    14641 tgccaccggg ctggttctat agccgcattg tggcaacctc ggtgctgggt gaagtgctgc
    14701 actgcaaagg cgaattgacc cgctcactgg cgctaatgca gcaaaccgaa cagatggcac
    14761 gccagcacga cgtctggcac tacgccttat ggagtttaat ccagcaaagt gaaattctgt
    14821 ttgcccaagg gttcctgcaa accgcgtggg aaacacagga aaaagcgttc cagctgatca
    14881 acgagcagca tctggaacag ctgccgatgc atgagtttct ggtgcgcatc cgagcacaac
    14941 tgttatgggc ctgggcacgg ctggatgaag ccgaagcgtc ggcgcgtagc gggattgaag
    15001 tcttgtcgtc ttatcagcca cagcaacagc ttcagtgcct ggcaatgttg attcaatgct
    15061 cgctggcccg tggtgattta gataacgccc gtagccagct gaaccgtctg gaaaacctgc
    15121 tggggaatgg caaatatcac agcgactgga tctctaacgc caacaaagtc cgggtgattt
    15181 actggcaaat gaccggcgat aaagccgccg ctgccaactg gttgcgtcat acggctaaac
    15241 cggagtttgc gaacaaccac ttcctgcaag gtcaatggcg caacattgcc cgcgcacaaa
    15301 tcttgctggg cgagtttgaa cctgcggaaa ttgttctcga agaactcaat gaaaatgccc
    15361 gcagtctgcg gttgatgagc gatctcaacc gtaacctgtt gctgcttaat caactgtact
    15421 ggcaggccgg acgtaaaagt gacgcccagc gcgtgttgct ggacgcatta aaactggcga
    15481 atcgcaccgg atttatcagt cattttgtca tcgaaggcga agcgatggcg caacaactgc
    15541 gccagctgat tcaactcaat acgctgccgg aactggaaca gcatcgcgcg cagcgtattc
    15601 tgcgagaaat caatcaacat catcggcata aattcgccca tttcgatgag aatttcgttg
    15661 aacgtctgct aaatcatccg gaagtgccag aactgatccg caccagcccg ttgacccaac
    15721 gtgaatggca ggtcctgggg ctgatctact ccggttacag caatgagcaa attgcgggcg
    15781 agctggaagt ggcggcaacc accatcaaga cgcatatccg caatctgtac caaaaactcg
    15841 gcgtcgccca ccgtcaggcc gcggtacaac acgcgcagaa actgctgaag atgatgggat
    15901 atggggtgtg agtttagccg gataatgcat tggatccggt ttacatcttt acatcattca
    15961 atactcaccc gcgttacgcc atctgtttct atcaaaccaa accgcaccgg caagaaacgc
    16021 tccaccacgg cgatattggt cagcagatgg catgaaggat gggcgaccgt aaattctccc
    16081 gcgcccgcca gcgccatcgg tagcaccagc tggtcggcga gatattcccc cactgccgcc
    16141 gggcttgcca ggtagcgttt cacctctttc actaactgcg cggctaccac ctcggcactg
    16201 acgcgctttt caccgacgac gaaaaagcgt tcagtgatat tttcactttc gacttccagc
    16261 gagacggtat tacccggccc ctgatcgcgc ggcaggttat gaatattctg ttcatgcagg
    16321 gaaaaactcc ccgccagtgt agcgatttca cgctcagcaa catggcgcgg cacaccagct
    16381 aatagaactt ctccacgcat ctgcacaatg ttcccgcgct cgccaagttg caaggtgtta
    16441 aacgatgcca cgggcgagac ttccgttgcc accacgccgc ctccggcagg ataaaaaccg
    16501 tgacgtaaca gcgtggtttg ctgatgaatt cctattttcg ccagcagcgg ctccagcacc
    16561 cggcggataa aatccgcagg cggcgccgac gggttatcgg tgcctccgct cacttcaaca
    16621 cgcgaaggtc catcggcaaa ccacagcgcg ggcagcaccg tttgcagcac cagcgtacag
    16681 cttccggcac taccgatagc aaagcggtac tcgccgccgc gcacggcgcc aggccggaag
    16741 accagacgct gcgaccccag ctccgcgcct tccaccgttg ccctacaaat ttccgcagcc
    16801 gcttttaccg cggtcagatg ctggcgcaac agccccggtt tcgcccgccc ggcacgaatg
    16861 ctggtgatgg taaatggctg gccggttatc atcgacaggc tcagcgccga gcgcagaatc
    16921 tgcccgccac cttcgccctg tgcgccatcc agcgcaatca tccttttcat catttatcct
    16981 tttacgcaca ccacctgacg cagggtatag ataacttcca ccagatcgct ttgtgccgtc
    17041 atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc ggcatcttta
    17101 cggcattcca catgcgcggt ggcacgaatt tgatcttcca cgctgaacag ttttttcgct
    17161 ttagttcggc tcattacccg cccggcaccg tggctgcacg aacagaacga ctcttcattt
    17221 cccagcccac ggacgataaa gctttttgct cccatcgaac cgggaataat tccatattga
    17281 ccagcacgcg cagacaccgc gcctttacgc gtcacgtaga tctcttcacc aaagtgctgt
    17341 tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt ttgtggctgt
    17401 ctgaccgttt tctgcgtaat gctctgcaat gccgttacca cgttttccat catcacatcg
    17461 cggttaaggc tggcaaaaag ctgcgcccag gccacggctt tcaggtaatc atcaaagtat
    17521 tccgtacctt ccataaagta cgccagatca cgcgacggca acgtctcaag cgtttcctgc
    17581 atctcttttt gtgccagatc gataaagtaa gtcccgatgg cgttaccaat tccgcgtgaa
    17641 ccggagtgca gcataatcca cacctggtcc gactcatcaa ggcagatttt aataaagtgg
    17701 ttaccggttc ccagcgttcc caggtgttta tagttattgg tattcaggaa acggggatat
    17761 ttttgcgtta accactgata accggcttca agctcagccc atttcgcatc gacgttgaca
    17821 ggcgggtttt cccatgcgcc tttatcacgt ttacaacgcc cggtagtacg cccgtgcggc
    17881 acggccgttt caatcgcctg acgcagctct gccaggtttt caggcaggtc ttccgccgtt
    17941 aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc cgccggaata
    18001 atcgcccctt tggtcgggat cacgctacca atggtggaat ctttacccag gtgtacatca
    18061 ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat aagttgctga
    18121 cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc attttcagtg
    18181 gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgctgtc ctgataattg
    18241 caaccgtcgt gccagaaaat taaataagta aattaatttt ttaattcatt gtttttaaaa
    18301 agattatatc tttatgtccg taaccggaga tttcccgcaa agccgattta ccgataatga
    18361 aatatcgtct tttataagga tatctaagat gcgtaaaaca gtagcttttg gctttgtcgg
    18421 aacggtgctg gattatgccg ggcgcggcag tcagcgctgg tcaaaatggc atccgacact
    18481 ctgtttatgc cagcaagaat cgttggtcat cgatcgactg gaattattgc atgacgcccg
    18541 ctcgtgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    18601 agtggtgagc attgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    18661 cctgcatgat ttcgcccgtg gttacaagtt tcagccagaa aaagaagact atttaattca
    18721 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    18781 tctacccgcc cggttgatac aaacttcacc accgcgcaaa aaagaacaac cgcgcggtgc
    18841 cggtgaagtg acgattatcg atctcgatct aagccgttat aacgccatcg ccagccgctt
    18901 tgccgaggaa cgccagcaaa cgcttgattt tcttaagtcc ggcattgcca cgcgtaaccc
    18961 ccacttcaac cgcatgattg agcagattga aaaagtggcg attaaatccc gcgcaccgat
    19021 cctgcttaac ggcccaaccg gtgcgggaaa gtcatttctg gcgcgacgta tatttgaatt
    19081 aaaacaggcg cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    19141 cggcgatacc gccatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    19201 ggaatctcgt gaaggtttat tacgcagcgc caacggcgga atgttgtttt ttgatgagat
    19261 tggcgaactg ggcgcagacg aacaggcaat gctgctaaaa gccattgaag agaaaacctt
    19321 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    19381 gcgcgatttg cgccagctgg ttgccgaagg caaatttcgc gaagatctgt acgcgcggat
    19441 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    19501 ccttgattat gaagtggagc gccacgccac actcactggc gacagcgtgc gttttaacac
    19561 cgaagcgcgg cgcgcctggt tggcttttgc gacctctccc caggcaacat ggcgcggtaa
    19621 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    19681 cactctggac gtggttgaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    19741 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    19801 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgcagccgg
    19861 acgacagctt tttgatgttt cgcgccaggg aaaagccagc gtcaatgacg cggatcggct
    19921 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    19981 cagttgaata tgatggtccg tcagcacctg cattacgctt actggcggcg gggcgtcggt
    20041 gtagacggca tctaccatgc tgatgctgcc catattgacc atcgcgttac ggccaaattt
    20101 cgagtgatcg acaaccagca taacgtggcg cgagttctca ataatggcgc gcttggtgcg
    20161 aacttcgtgg taatcgaact ccagcagcga gccgtcgcta tcgatgccgc ttatccccag
    20221 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    20281 gccatcgcgg ctgcgtaatt cgccaccagc gagaataatg cggaaatctt cttttaccat
    20341 caacgtgtta gcaacattga gattgttggt gacaatacgc aaattgctgt gattaagcag
    20401 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagtgtcg agccgttggg
    20461 gatttgctcc gccactttgc gggcaatcct ctctttttct tcggtctggg tggctttgcg
    20521 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcaccgccat ggtgacgcag
    20581 gatcaggttt tgctccgcca gctcattgag gtcgcggcga atagtctgcg ggctgacgga
    20641 gaaatgctct accagctctt cagtactgac ataaccctgc tgtttaacca gttcgataat
    20701 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    20761 cattgagcga atcaacaaaa gccatcgcta aacccacggc taacccggcg atgtgtgctc
    20821 cgttcgccat cgacatccca aacaaatcaa accatccggc gataatccag atcagcgcaa
    20881 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgctca ccacgtagcc
    20941 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    21001 atttttgctg cacatagccg cttaacaggg cactaatgag agtaataaca attagcttac
    21061 cgctaccgag gcgtttttcc accgcaccgc cgagatacca ccaccagagc aggttaaaga
    21121 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    21181 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    21241 gcatggcgat aaacaccacc acgcaggcga tcatcaccac ccaggttacc ggacctgcgc
    21301 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg cagaccactg ccggtatggc
    21361 ccgcctgcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    21421 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    21481 gttgttgaat cgtaaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    21541 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    21601 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gacgctgcca
    21661 ggcttcaaaa ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    21721 cgccgcgcct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    21781 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    21841 tcccattgcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    21901 cttctggtgc gcgtcggcaa cgttaataca ttcgaacaga tccatgcgtc tctctttctt
    21961 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    22021 atgttataca tatcactcta aaatgttttt tcaatgttac ctaaagcgcg attctttgct
    22081 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    22141 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    22201 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    22261 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    22321 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    22381 ttcccaatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgg
    22441 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaaccggt
    22501 ttgcgttttg gcgcaaactc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    22561 gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt ggtgcgtaaa
    22621 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    22681 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    22741 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    22801 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgtgt gcatacccag
    22861 aagcaggcct acattctgca aaacgaagat aaacgtattg tgttcgtgat cccgtggatg
    22921 gacgaatttt ccatcatcgg caccaccgac gtagagtata aaggcgatcc gaaagcggtg
    22981 aagattgaag agagtgaaat caactatctg ctgaaagtgt ataacgcgca ctttaaaaaa
    23041 cagcttagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    23101 gagtccgact cgccgcaggc tattacccgt gattacaccc ttgatatcca tgatgaaaac
    23161 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    23221 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    23281 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc agctcgtctg
    23341 cgccgtcgct atccattcct gactgaatcg ctggcgcgtc attacgcacg cacttacggc
    23401 agcaacagcg agctgctgct cggcaatgcg ggggcgataa gcgacctcgg ggaggatttc
    23461 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    23521 accgacgatg ccctttggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    23581 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    23641 ggatgatgcc cggttgaagt gcaaccgggc tatttggatt ccttagacta ttcattgtaa
    23701 ataattgaat aacggcccct gagctaaaat cgccaaactt aagcacgtta cccaatcgcc
    23761 ccacttcttc ctctggtttc tattttttct gcaccaatgg caataatatc ccaatgcaaa
    23821 accaagctgt gcactaatcg ggccgacaaa atcagtccaa ggctgcggcc aaaaagggga
    23881 aaaattcagg ggaggccaga caaacgtcag aaagaaattt cggatatttc ccagcaacgc
    23941 tgatttctct ggagaaatat aaagaatgaa attcgagaga attaaccaga aatagaccgg
    24001 cgaaaagacc agccagcaat gcagtctgtt ttttaattca ggaggcgttc tgcgggagaa
    24061 ttgccaaccg atgccagcca ttacac
//
LOCUS       DAJTSL010000053        23482 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTSL010000053 DAJTSL010000000
VERSION     DAJTSL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.053"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(297..611)
                     /gene="pspE"
                     /locus_tag="OO433_001868"
     CDS             complement(297..611)
                     /gene="pspE"
                     /locus_tag="OO433_001868"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6050370.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(686..907)
                     /gene="pspD"
                     /locus_tag="OO433_001869"
     CDS             complement(686..907)
                     /gene="pspD"
                     /locus_tag="OO433_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6050371.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(916..1275)
                     /gene="pspC"
                     /locus_tag="OO433_001870"
     CDS             complement(916..1275)
                     /gene="pspC"
                     /locus_tag="OO433_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6050372.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1275..1499)
                     /gene="pspB"
                     /locus_tag="OO433_001871"
     CDS             complement(1275..1499)
                     /gene="pspB"
                     /locus_tag="OO433_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6050373.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1553..2221)
                     /gene="pspA"
                     /locus_tag="OO433_001872"
     CDS             complement(1553..2221)
                     /gene="pspA"
                     /locus_tag="OO433_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6050374.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKESGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2388..3365
                     /gene="pspF"
                     /locus_tag="OO433_001873"
     CDS             2388..3365
                     /gene="pspF"
                     /locus_tag="OO433_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6050375.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPATVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFATQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3485..4750)
                     /gene="puuE"
                     /locus_tag="OO433_001874"
     CDS             complement(3485..4750)
                     /gene="puuE"
                     /locus_tag="OO433_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR6050376.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(4788..6057)
                     /gene="puuB"
                     /locus_tag="OO433_001875"
                     /pseudo
     CDS             complement(4788..6057)
                     /gene="puuB"
                     /locus_tag="OO433_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
     gene            complement(6059..7546)
                     /gene="puuC"
                     /locus_tag="OO433_001876"
     CDS             complement(6059..7546)
                     /gene="puuC"
                     /locus_tag="OO433_001876"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR6050377.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FSDCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWMRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(7821..8378)
                     /gene="puuR"
                     /locus_tag="OO433_001877"
     CDS             complement(7821..8378)
                     /gene="puuR"
                     /locus_tag="OO433_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6050378.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8405..9168)
                     /gene="puuD"
                     /locus_tag="OO433_001878"
                     /pseudo
     CDS             complement(8405..9168)
                     /gene="puuD"
                     /locus_tag="OO433_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            9380..10798
                     /gene="puuA"
                     /locus_tag="OO433_001879"
     CDS             9380..10798
                     /gene="puuA"
                     /locus_tag="OO433_001879"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6050379.1"
                     /translation="METNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10947..11112
                     /locus_tag="OO433_001880"
                     /pseudo
     CDS             10947..11112
                     /locus_tag="OO433_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11102..12487
                     /gene="puuP"
                     /locus_tag="OO433_001881"
     CDS             11102..12487
                     /gene="puuP"
                     /locus_tag="OO433_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6050380.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSINLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12621..12866
                     /locus_tag="OO433_001882"
     CDS             12621..12866
                     /locus_tag="OO433_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6050381.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13179..14822
                     /gene="sapA"
                     /locus_tag="OO433_001883"
     CDS             13179..14822
                     /gene="sapA"
                     /locus_tag="OO433_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6050382.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLKFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14819..15784
                     /gene="sapB"
                     /locus_tag="OO433_001884"
     CDS             14819..15784
                     /gene="sapB"
                     /locus_tag="OO433_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6050383.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15771..16661
                     /gene="sapC"
                     /locus_tag="OO433_001885"
     CDS             15771..16661
                     /gene="sapC"
                     /locus_tag="OO433_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6050384.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMLAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16661..17653
                     /gene="sapD"
                     /locus_tag="OO433_001886"
     CDS             16661..17653
                     /gene="sapD"
                     /locus_tag="OO433_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6050385.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17655..18461
                     /gene="sapF"
                     /locus_tag="OO433_001887"
     CDS             17655..18461
                     /gene="sapF"
                     /locus_tag="OO433_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6050386.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18529..18882
                     /locus_tag="OO433_001888"
     CDS             18529..18882
                     /locus_tag="OO433_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6050387.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19250..20038
                     /gene="fabI"
                     /locus_tag="OO433_001889"
     CDS             19250..20038
                     /gene="fabI"
                     /locus_tag="OO433_001889"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6050388.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20183..21310
                     /locus_tag="OO433_001890"
     CDS             20183..21310
                     /locus_tag="OO433_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6050389.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTARDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     TAQFSPLLEHEQSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21378..23312
                     /gene="rnb"
                     /locus_tag="OO433_001891"
     CDS             21378..23312
                     /gene="rnb"
                     /locus_tag="OO433_001891"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6050390.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGAIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23390..23482)
                     /locus_tag="OO433_001892"
                     /pseudo
     CDS             complement(23390..23482)
                     /locus_tag="OO433_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5306 a         6209 c         6113 g         5854 t
ORIGIN      
        1 gacgcgttac aagtttctgt aaatgagttg cggtaaatgt tccaccgtag atttcgtcca
       61 ggtaatcagt aattttctgt ttcatgttgc ctttcagtgg tagcgatccc atgaaacgag
      121 agactactcc gcagaaaata aactgtcaat tgagcacaag ggttttagtc acgcaggaaa
      181 tcatttttta ccagggaaaa agcgtatttg tgagctgacg caaataatct atggggatgt
      241 aaataaggtg tgacagaaaa aaaacggcgc ataagcgccg ctcatggtga attcttttaa
      301 cctttgacct tcggcattgc gatgtctttc aagccaccgg cgttctcaac gtgggtatat
      361 cccatctcgc taaggatctc ttttgcttgc cctgactggc gtccggcatt gcaatacact
      421 ttcacggtgt cgtttttatc cggaacggcg gtggcaatgc gctctttcac ttccttcagg
      481 ggaatattga tggccccctg aacgtgctct tgctgatact gctctggaac acgaacatcg
      541 atccagtgtt cagcggcgaa aacgggcagt gaaaacacca gtgccagagc aagtaagcct
      601 tttttaaaca tgtaagcgtc cttctatgga tgaaaattgc catccattat acgcctgaga
      661 tttaacaatt tttgcgaaac tcccctcacc ttttataacg ctgtgccagt ttattagcag
      721 cccgacttaa cagcggttcc agcgccacag ccagcaacat tttcagcggt cggcgagcaa
      781 ctgattttat cgcccagccc gctacacccg ccgggccata gcgcagtgcg gtaagaagta
      841 ccagcttgcc tgctaatttg aaaccaggct ttaccttttg cccggcctgt tgccagcgag
      901 tattcataac tttcctcaca gttgacggaa acggctacgt aacgtgaaag tatcggaagt
      961 gacataacgt tccatctcgc gtaaacgcgt ttcacttgcc gccagttcac ggtcgacttc
     1021 atccagcaat tcgctgctgg agggtagctg ctcaccaaag gccatgttgt ccggcattgg
     1081 atcaagcgca aatgacaaaa tgatgtaagc aaccagggta aacagcgcca gaccgaagaa
     1141 aatcgacagc accaccagga tacgcaccag ttttaccggt acatcaaaat agttggcaat
     1201 cccggcgcag acgccgcgga ccatgccctg ctgtggaata cgccataatt ttttattgag
     1261 attaatgccc gccattagcg atccctccag ttcggatgtt ctgcatcaag aatagattcc
     1321 agcgcctgaa tacgttcgcg catccgtttt gcttcatcag ccagttgcgc taatcgctgc
     1381 tgctcacttt gcgacaattc actgcgacca gaacgattgc tgtaatgcag ccataaccag
     1441 atcggtaaaa caaacagcac aaaaatggtt aacggaatag ccagaaatag cgcgctcata
     1501 agtactcctt agacgatgag cggcggcgcg ttcggacgcc gcctagatgt tattattgat
     1561 tgtcttgctt cattttggct tttaattgtg ccagttgttc gctgattgca tcatcggctt
     1621 tcagttcggc aaactgatcg tccagcgatt tttgtttacc gaagctgtgg ctttctgctt
     1681 ccgcttccat ctggtcaata cgacgttcga aagattcgaa acgagccatt gcttcatcca
     1741 gtttgccact gtccagctga cgacgcacat cgcgcgacga gtttgccgcc tgatggcgta
     1801 acatcaatgc ctgctggcga gcgcgtgttt cgctcaattt gttttccagc tcaccgctct
     1861 ctttcttcat gcgtgccagc gtatcgtcca ccagcgtcac ttcatgttcc agactcttaa
     1921 tcagatcggt cagtttctgt ttttcaatta acgccgcacg tgccaaatcc tctctctctt
     1981 tcagcagcgc cagttcggct ttttcctgcc attcaacttc acgcgccgac gcttgttcaa
     2041 tacggcgagt cagctgtttc ttttctgcca acgcacgcgc cgaagtagaa cgtacttcaa
     2101 ccagtgtatc ttccatctcc tggatcatca gacgcaccag tttctgtgga tcttccgctt
     2161 tctctaacag agcgttgatg ttggcattca cgatgtcggc aaagcgagaa aaaataccca
     2221 taatgttgtc ctcttgattt ctgcgttcag gattgtcctg ctgaactgtt aatacaattt
     2281 gcgtgccaat tttttatctt tttgatttat aaagatctga ttgaagaatc atcagcaaca
     2341 tgccaggatg aattagcgaa ttacactaac aagtggcgaa tttcatcatg gcagaataca
     2401 aagataattt acttggtgag gcgaacagct ttctcgaagt gctggaacag gtttcgcatc
     2461 tcgcaccgct ggacaaaccg gtgctcatca tcggcgaacg cggcaccggt aaagagctga
     2521 ttgccagccg cctgcattat ctctcctccc gttggcaagg gccgtttatt tcccttaact
     2581 gcgcggcgtt aaatgaaaat ctgctggatt ccgaactgtt tggtcacgaa gcgggggcgt
     2641 ttaccggtgc gcaaaaacgt catccaggga gatttgaacg tgccgacggc ggtacgctat
     2701 ttcttgatga actcgctacg gcaccgatga tggtgcagga gaaattattg cgcgtgattg
     2761 agtacggtga actggagcgc gttggcggca gccagccatt gcaggtgaat gtgcggttgg
     2821 tatgcgcgac gaatgccgat ctcccggcga cggtcaatga aggcactttt cgcgctgacc
     2881 tgctcgaccg actggctttt gatgttgtac aactgccacc actgcgcgag cgcgaaagcg
     2941 acataatgtt gatggcagaa cactttgcca cccagatgtg tcgggaaatc aagctgcctc
     3001 tgttcccggg gtttacggag cgcgccagag aaacattgct gaattatcgt tggccgggaa
     3061 atattcgtga attgaaaaac gtggtggaac gttcagtgta tcgccacggc accagcgatt
     3121 atccgcttga tgacatcatt attgatccct ttaaacggcg tccgcctgaa gacgctatcg
     3181 ccgtttcaga aaccacctcg cttccaacac tgccgctgga tttacgtgag tttcagatgc
     3241 agcaggaaaa agagttgctg caactcagtt tgcaacaagg gaaatataac cagaagcgcg
     3301 cggctgaatt actggggtta acttatcatc agttccgcgc gttgttgaaa aagcaccaga
     3361 tttagcgaag ttgagcaata caacttgccg gatgcggcgt gaatgcttta ttcggcatac
     3421 ataatcaata agtcgtttgc aggcctgata agcgcagcgc atcagacatt attgcgttgg
     3481 gctattaatc gctcagcgca tcctgcaaaa ttttcattgc cgcatcgaat tgcgcatccg
     3541 gaatggtcag cggatacagg aagcgaatca cgttgccgta tgcgccacag gtcagcagga
     3601 gcagcccctg cgccagcgcg cgttgctgga ttttctgtgc aatcgccgct gacggctcgc
     3661 ccgtttgcgg atcgttaaac tctgccgcaa tcatcgaccc caggccgcgt accgcagcaa
     3721 tggccggaac gctttctttg gcatcaatca acgtgttttt gagacgctgg cccagttgat
     3781 tcgcgcgttc gcagagtgat tctttgtcga taatattgag caccgcgtgc gcggcagcca
     3841 ccgccagcgg gttcccggcg taggtgccac ccaacccgcc cggcgcgggc gcgtccataa
     3901 tattcgcgtt accgaccacg cccgaaagcg gcatcccgcc cgcgaggctt ttcgccatcg
     3961 tcattaagtc cggcttatcg gcgtaatgat ccatggcaaa cagtttaccg gtacgcgcaa
     4021 agccgctttg cacttcatcg gcaatcatca caataccgtg ctcgtcgcac aggcggcgaa
     4081 tggcggcaac cagctctttt ggcgcaacgt tgaaaccgcc ctcgccctgc accggttcga
     4141 aaataatggc cgccacctgc ttcgcttcaa tgtccgattt aaacaagcgt tcgatggcgt
     4201 cgagggagtc ctgtgttgaa atgccgtgta aatctgacgg ataaggtacg tgatacaccg
     4261 aaccagggaa cgggccgaag ccgattttgt acggcgcaac ttttccggtc agcgccatgg
     4321 tcatatatgt acgaccgtgg aagccgccgc taaacgcaat cacgccaggt cgtccggtat
     4381 gggcgcgagc aattttcacc gcgttttcca ccgcttccgc gccggtagtg aagaatgcgg
     4441 ttttagcctg cccgctcact ggggcaaggg cgttaatttt ctccgccagc gtgacgtagc
     4501 tttcgtacgg cacaatctga tacgccgtgt gggtaaactg ttgcaattgc tgctccaccg
     4561 ccgcgaccag atcagggtgg cgatgcccgg tattcagcac cgcaatgcct gcggcgaaat
     4621 cgatgtattc gttgccctct acgtccttca gcgtagcgtt ttctgccgac tgggcgaaga
     4681 agttacacat cacgccaacc ccgcgtgggg tagcagaaag acgacgctga tggaattcat
     4741 tgttgctcat gtttcacacc ttttgactct tcactgttaa tcggaaatta aacgcccaga
     4801 cgatcgcgca ggctgtaata agccgcgccc atcgcggtaa atggcacgcg cagcgtgcgc
     4861 ccgccgggga acgggtaatg cggcagattg gcgaaggcat cgaaacgctc ggcgtcaccg
     4921 cgcagcagtt cagcaatcaa acgcccggcg aggtgggtac aggtgacgcc gtggccgctg
     4981 tagccctgca tgtaatagat gttggtatcg aggcgaccga actgcggcat ccgtgaaagg
     5041 gtcagcagga agttgcccgt ccagcgatag tcgattttga cgcccttcag ctgcgggaag
     5101 gttttcagca gtttcggcac aacaagacgc tcaacgtcat ccgggtcgcg cgcgccgtag
     5161 accacgccgc cgccgtacag caaacggttg tcggcggtaa gacggtagta atcaagcaga
     5221 taattacagt cttccacaca gtagtttttc gggatcagcg aacgggctaa atcttccgac
     5281 agccgttcgg tggtgatcac ctgcgtgccg cacggcatgc tgcgtttcgc cagttccggc
     5341 tctactttat cgcccagata cgcattcccg gcgacaatca catacttcgc cgtcacctga
     5401 cctttggcag tgcgcacaac ggctggcgtg gtgtgctgga tctgcgtcac ggcagaaagt
     5461 tcatacacgc gcccgccgtt gaggcggatg gcgtccgctt cgccaatggc gaggtttagc
     5521 gggtgaatat gcccaccgct gtgatccagc agcgcaccgg tatagcgatc gctggctact
     5581 tcacggcgaa tggcgttggc gtccagcaat tccagctgtt tattgccgta gcgttcccag
     5641 ttctctttct gctcttcaag tgtggcgagc tgtttatcat tcatcgccac aaacaggctg
     5701 ccggggcggt agtcgcagtc aatctgataa cgtttgatac gttcgcggat gatctcacca
     5761 ccttcgaaca tcatgctgcc gagcataggg gctgtgtcca tgccgtagct tttttcgatc
     5821 acgtcgatgt cgcggttgta ggagttcaca agctgcccgc cattgcgccc gcttgcgcca
     5881 aagccgatgc gtgaggcttc gagaaccact acgtcaaagc ccgcttccgc cagatgcagc
     5941 gcggaggaga gcccggtata gccgccgcca accacgcaaa cgtcgcaggt gattgactca
     6001 ttcagggtgt cgaatggcac gtatttatgc gtaataactg ctggtatgtt cggtcatttc
     6061 aggcctccag gcttatccag atggttttca gttcggtgaa tttttcaagg gcatgcaggg
     6121 atttgtcgcg accgttgccg ctctgcttat agccgccaaa cggcacggtc atatcgccgt
     6181 cgttgtagtt attgacgaag acggaaccgg ctttcaggcg gcggctcata cggtgcgcgc
     6241 gggagaggtc gcgcatccat accgccgcgc caaggccgta ctggctgtcg ttggcaagct
     6301 gtagcgcctg ttcttctgat gtgaaacgcg tgaccaccag caccggaccg aaaatctctt
     6361 cgcggcttaa ggacgcattc gggtctacat ccacaaagat ggtcgggccg atggcggcag
     6421 ccagcccggc gttacggcca tccagcagca gttgcccttt gccttcgccc tcacgaataa
     6481 agctatggac cgagtcggcg tgggcgcagt cgattaaagt gcccatggtg gttgcggggt
     6541 caagcggatg gcccggctgc cagttttgcg cctgctgttt taacagggct aagaattcat
     6601 cggcgatgct ctcttccagc aacaggcgtg ttccggcgat gcacacctga ccctggttgt
     6661 agaagatgcc tgctgcggtg gagctcgccg ctttttgcag atccgaacaa tcggagaaaa
     6721 cgatgttggc gcttttgccg cccgcttcca gccagacgcg tttcatgttg ctgtcgcccg
     6781 catctttcag cagctgtttc ccggtacggg ttgaaccggt aaaggcaatg gcgtcgatat
     6841 cgttatgacg cgacagcgcc tgccccgctt catgaccaaa acccgtcacc acgttcaaca
     6901 caccatccgg caagcctgct tctttcgcca gccccgcgag acgaatcgca ctgagcggtg
     6961 atttttcaga cggttttaga atcacgctgt ttcccgccgc cagcgccggg ccgagtttcc
     7021 agcaagtcag caacagcggg aagttccacg gcacgatggc ggcaatcacg ccgaccggtt
     7081 cacgcacgat catcgccagc tcatggctac tggtggtcgc cacttcgcca tacactttgt
     7141 cgatcgcttc ggcgtaccag cgaatggcgc gcgccgcgcc gggaatatca tcacgcagac
     7201 tgtgacgaat cggtttgccg gtgtcgagag tttccagcag tgccagctct tcggcgtggg
     7261 cttccattaa atcggcgagt ttattcagta ccgctttacg tttagccgga gaagagagtg
     7321 accagtcgcc gcgttcaaat acgccgcgtg ctgcgctcac cgcacggtcg atatcgacgc
     7381 tcttgccgcg ggcaattttc gccagcggtg cctgggtgac tggatcaacg gtttcaaagg
     7441 tttcattttc cgccgcagca gtatattcac cgttaataaa taagcggttt tcaatggcga
     7501 gacttaacgc tttatcctgc cagtaagcca gatgatgaaa attcattatg actcctgttt
     7561 cacgtctatc agatatatgc agtttgtgaa cgtcagggcg cagagatccc cgggccgtat
     7621 gcccgtttat ttctggcgac attctttata tacacttcat ccttcaagcc gcctctgcct
     7681 tggctgcgtt cgctcccccg aatcacttac ctgagtaagc tcatcgggat tcgctcactt
     7741 gccgccttga tgcaacttga atgattttgt gtatagattg ccgggcgcat tgcgcttacc
     7801 cggcttacag aacaaaaaga ttaaaacgtg gtgggcgtat gggcgctgat aattcggcaa
     7861 atacctgccg acgtattact gaaactgtgc gggatgccgg tattaatggc ataactttga
     7921 cccgcgacga ggtggtaatc ctgaccatta atcgtcagaa caatttcacc ttccagtact
     7981 gtgcctattt cctcaccctg atgcttaatt ctttccccag tggttgtgcc cggctggtac
     8041 gtttcaaaga tcatcgccag cgtgcgattc gggttaccgt tatgaaccag cttcattgac
     8101 acaccctgac tgcccatctc aattaagtcg tcctgattaa tgacgacctg cggctcatca
     8161 ggtttttccg gctcggaaaa gaattccgag agtgacagac catacacctt cagcagcttt
     8221 tgcagcgtac tgatggcagg gctgacttta tcttgttcta tcgtactgat agcactgtga
     8281 gtcagcccgg agagttcggc ggcacgacgt tgtgaaagcc cctgctgctg gcggatttcc
     8341 gacaagcgtt ttcctggcgc cagtccctca tcactcatat ttgcatttcc ttaaactgta
     8401 gtggtcagag tcgctgtttt tcagcgatat ggtgctgaca agcggtgata aagccctcga
     8461 acaatatacg cgaaagagcg tactcgctac tgttccattc cgggtgccac tgtacgccca
     8521 gcgcaaaagg atgattgatg acgctaaccg cctcaactaa gccatccggc gagcgagctt
     8581 caacacgcaa ccgtgggcta acgaccttcg ccccttgtcc atgtagagag tttacccaaa
     8641 agttgctaca ttcaggtaac aacgcagaca gtaatccccc ctcttcaacc tgaacttcat
     8701 gcgacggtgc atattgctgt tccaccggca gttcggatct tcccgatgtt ccagcaattc
     8761 aggctgttcg cacagcttgc gatgcaacga accaccggtt gccaccacca gttcttgtaa
     8821 accccggcag atggcgaaaa tggggatgcg cctttcgagt gcggcattaa ttatcgccat
     8881 gctcagaaga tcacgcccgg gatcggcgtc aggctcatcg ccgttttcac catataggtg
     8941 cggctgcaca ttgctgggac taccaggaag ataaatgcca tcgagtttcg gcaaaagttg
     9001 ttcaagtaac gacggttccg ccagcgcatg tggtagcgca attggcaagc cgcctgcgtg
     9061 gatgatggca ttcaggtact tttcttgcag agtctgggtc gcatgaccct taagcctgtt
     9121 cctgcacatt acgacaccga taaccggatt gttcattata tttttcatgc tcaatctcac
     9181 aaagtggact aaattatcgc cattactgcg ttttaaatga cctttatgtt caatattttt
     9241 tcaatctagc agtgggtttt catttttgca aactaaattt aacatttagc aaacatttag
     9301 tttgcataca gattcgaatg gtggtcatta tattttacgc tttgataacg agcggaaaac
     9361 aaaccaaagg cgaagaatca tggaaaccaa tatcgttgaa gtagagaact ttgttcagca
     9421 gccagaagag aggcggggta gcgcctttac gcaggaagtg aaacgctacc tggagcgcta
     9481 cccgaatacg caatatgttg atgttttact tactgatttg aatggttgct tccgtggaaa
     9541 acgtatccct gtttcaagcc tgaagaagtt ggagaaaggg tgttatttcc cggcctcggt
     9601 gtttgcaatg gatattctgg gcaacgtagt agaagaagcg ggtctgggtc aggaaatggg
     9661 cgagccggat cgtacttgtg ttcctgttct cggttcctta accccttctg ccgccgatcc
     9721 agagtttatc ggtcagatgc tcctgaccat ggtcgatgaa gatggcgctc cctttgacgt
     9781 tgagccgcgg aacgttctta accgcctgtg gcagcagcta cgccagcgcg gattgttccc
     9841 ggttgtagcg gtagagctgg agttctattt actggatcgt cagcgcgacg ctgaagggta
     9901 tctgcaaccg ccctgcgcgc ccggcaccga taaccgcaat acacaaagcc aggtttactc
     9961 cgttgataac ctcaaccact tcgccgacgt gctcaatgat attgatgaac tggcgcagtt
    10021 acagttaatt cccgccgacg gtgcggtcgc cgaagcctcg ccgggtcagt ttgaaatcaa
    10081 cctttaccat accgataacg tgctggaagc ctgcgatgat gcgctggcgc taaaactcct
    10141 ggtgcgtctg atggcagaaa agcataagat gcactccact tttatggcga agccgtatga
    10201 agagcacgcg ggcagcggga tgcatatcca tatcagtatg caaaataatc gtggcgagaa
    10261 tgtgctttct gacacagaag gcgaagattc gccgctgctg aaaaagatgc tcgccgggat
    10321 gattgacctg atgccgtcgt cgatggcgtt gctggcacca aacgtgaact cgtatcgccg
    10381 cttccagccg ggaatgtatg tgccgacgca ggcgtcgtgg ggccataaca accgcaccgt
    10441 cgccctgcgt attccgtgcg gcgaccgtca taatcaccgc gtggaatatc gcgtggcggg
    10501 tgccgatgcc aacccatatc tggtgatggc agcgattttt gccggtattt tgcatggcct
    10561 tgataacgag ctgccgttgc aggaagaagt cgaaggcaac gggctggaac aggaaggctt
    10621 acccttcccg attcgccaga gcgatgccct gggtgagttt atcgagaatg atcacctgcg
    10681 ccgctattta ggcgaacggt tttgccatgt gtatcacgcc tgtaaaaatg atgagctgtt
    10741 gcagtttgag cgcctcatca cagaaacaga aattgagtgg atgttgaaaa acgcatagta
    10801 ccccttgcgg ggttgtttca tcgacctgag cggagccagg cctgacattt gctggtaatc
    10861 cagacatttc ccggatgtcg cgttatggag cgcaggcggc aacgggcttc cgttaacgat
    10921 taagactgtg acgaggaaat gagatgatgc cgatgattaa atccccgcat ggcgagggag
    10981 gctgcgtatg cgcccctcct gctactgact ggactccgtc ccctttgctt cccttgctaa
    11041 acaggtttga tttcaggtcg acacgaccgc aaaccttatt acgcaggggg aggcagcaat
    11101 tatggctatt aattcaccac tgaatattgc tgcgcaaccc ggcaaaaccc gtctgcgaaa
    11161 atcactgaaa ttgtggcagg tggtgatgat gggtctggcc tatctcacgc cgatgaccgt
    11221 atttgatact tttggtattg tctccggcat tagcgacggt cacgttccgg cgtcctattt
    11281 gctggcgctg gcgggcgtgc tgtttaccgc tatcagctac ggcaaactgg ttcgccagtt
    11341 tccggaggcc ggttcggcct atacctacgc gcaaaagtcg attaacccgc acgtcggatt
    11401 tatggtcggc tggtcatcgc tgctggatta tctctttttg ccgatgatca acgtcttgtt
    11461 ggcgaaaatc tatctctccg ccctcttccc ggaagtgccg ccgtgggtgt gggtagtaac
    11521 cttcgtcgcc attttaaccg ccgcgaatct gaagagcatc aacctggtcg ctaacttcaa
    11581 taccctgttt gtactggtgc aaatctccat catggtggtg tttatcttcc tggtagttca
    11641 gggactgcat aaaggagaag gcgttggcac cgtctggtca cttcagccgt ttatcagcga
    11701 gaacgcgcac ctgatcccga ttattaccgg ggcgacgatt gtctgtttct ccttcctcgg
    11761 tttcgatgcg gtgaccacgc tttcggaaga gacgccggac gccgcacgcg tgatcccgaa
    11821 agccatcttc ctgacggcgg tctacggtgg cgttatcttt atcgcggcgt cgttctttat
    11881 gcagctgttc tttcccgata tcagccgctt taaagatccg gatgccgcac tgcctgaaat
    11941 tgcgctgtac gtcggcggca agctcttcca gtcgattttc ctctgcacca cgtttgtgaa
    12001 cacgttagcc tctggcctgg cttcacatgc cagcgtgtcg cgtctgctgt atgtgatggg
    12061 gcgtgacaat gtgtttccgg agcgcgtgtt tggctatgtg cacccaaaat ggcggactcc
    12121 ggcactcaac gtcattatgg tcgggattgt cgcgttgtcg gcgctgttct tcgatttagt
    12181 taccgccaca gcgttaatta acttcggtgc tctggtggcg tttaccttcg tgaatctgtc
    12241 ggtgtttaat catttctggc ggcgtaaagg aatgaataaa agctggaagg atcacttcca
    12301 ctatttgctg atgccgctgg ttggcgcgct gacggtgggt gtgctgtggg ttaacctcga
    12361 gtcaacgtca ctgacactcg gtctggtatg ggcttcgtta ggcggcgcat atttatggta
    12421 tctgatccgc cgctatcgca aagtgccgtt gtatgatggt gacagaacgc cggtgagtga
    12481 aacgtaatag tcggatgcgc ggcgcattat cctgccggag ggtaatgcac cgcgccatta
    12541 actataaaaa gatgtatgac ccggatcacg agtgctatca tccgccctta tgcttagcgc
    12601 attaacccca ggggcaaata atgaaccacg atatcccgct aaaatatttt gatattgccg
    12661 atgagtacgc gaccgaatgc gcagagcctg tcgcagacgc tgaacgcaca ccgctggccc
    12721 actacttcca gttactgctc acccgtttga tgaacaatga agagatcagc gaagaagccc
    12781 aacatgaaat ggctgctgaa gcggggatta atcccgtacg cattgatgag atcgcagagt
    12841 ttttgaatca atggggtaat gagtaattat ttaattgcag acgatcggtc agtatcgttg
    12901 tccggataaa gcgttcacgc cgcatccggc taacaatgac acgcactgca gcgtgcttta
    12961 atgggtttct cacgatcgta ccgctctgct cctcgctgag ttgttctgtg agttatagtt
    13021 atagaggaag cacgggtacg aatgttgaac agatggcgac gccacctgtt caacgaccaa
    13081 ccacacggcc ctcattacga cccctgaaca caaacggtaa tggcaaatgt cattaaatat
    13141 tagtaagata gcctctttac ataaaatccc ttaatattat gcgccaggta ttatcgtctc
    13201 ttttggtgat tgctggactt gtgagtggtc aggcaatcgc cgcgcctgaa tcccccccgc
    13261 atgctgatat ccgcgacagc ggttttgtct attgcgtcag cgggcaagtc aacaccttta
    13321 acccatccaa agcgagcagt gggttaattg tcgataccct tgccgcccag ttttatgatc
    13381 gactgctgga tgtcgatccc tatacctatc gcctgatgcc ggaacttgcc gaaagctggg
    13441 aagtactcga caacggcgcg acctatcgct tccacctgcg tcgcgatgtt ccgtttcaaa
    13501 aaaccgactg gtttactccc actcgtaaaa tgaatgccga cgatgtggtg tttaccttcc
    13561 agcgaatttt tgaccgcaac aacccgtggc ataacgtcaa cggcagcaac ttcccctact
    13621 tcgacagcct gaaatttgcc gataacgtga aaagcgtccg caaactggat aatcataccg
    13681 ttgagttccg actcgcccag ccggatgctt cttttttgtg gcacctcgca acccattatg
    13741 cttcggtcat gtcggcagaa tatgcccgga agttagagaa agaagatcgc caggagcaac
    13801 tcgaccgtca accggtcggc accggtccgt atcagttgtc ggaataccgc gccgggcaat
    13861 ttattcgcct acaacgtcat gatgacttct ggcgcggtaa accgttaatg ccgcaggtag
    13921 tggtggattt aggctccggc ggcaccggac gtctgtcgaa actcctgacc ggggaatgcg
    13981 acgttctggc ctggcctgct gccagccagc tatccatttt gcgtgacgac ccgcgcttgc
    14041 gtttaacgct gcgtcctggg atgaacgtcg cctatctggc atttaacacc gccaaaccgc
    14101 cgctaaataa tcccgctgtc cgccatgcac tggcactggc gattaataac cagcgcctga
    14161 tgcaatccat ctattatggt acggctgaaa cggcggcctc tattttaccg cgcgcctcgt
    14221 gggcctatga caacgaggct aaaattactg aatacaatcc ggcgaaatcg cgcgaacagt
    14281 tgaaggcgtt ggggctggaa aacttaacgc tgaaactgtg ggtgcccaca cgttcgcagg
    14341 cgtggaaccc cagtccactg aaaactgccg aactgattca ggcggatatg gcgcaggttg
    14401 gcgtaaaagt ggtgattgtg ccggtagaag gtcgctttca ggaggcacgg ttgatggata
    14461 tgagccatga tctgacgtta tccggttggg cgacggacag taacgacccg gacagtttct
    14521 tccgtccttt actgagctgc gcggcaattc attcacagac caacctcgcc cactggtgcg
    14581 atccgaaatt cgacagcgtg ttgcgtaagg cgctctcctc gcagcagctg gcggcgcgta
    14641 ttgaagccta tgacgaagcg cagagtattc tggcgcagga attgcccatt ttgccgctgg
    14701 cgtcgtcatt gcgtttgcag gcctatcggt acgatatcaa aggtctggta cttagcccgt
    14761 ttggtaacgc ctcctttgct ggggtgtatc gcgagaaaca ggatgaggtg aaaaaaccat
    14821 gattatcttc accttacgcc gcattttgtt attgattgtc accttgttcc tgctgacctt
    14881 cgttggcttc agcttgagct atttcacccc tcatgcgccg ttacaaggcg cgtcactgtg
    14941 gaatgcctgg gtgttctggt ttaacggcct gatccactgg gattttggtg tttccagcat
    15001 taacggtcaa ccgattgccg aacagttaaa agaggtcttc ccggcgacga tggaactgtg
    15061 catcctcgcc ttcggttttg cgctgattgt tggcatcccc gtggggatga ttgccgggat
    15121 tacgcgccat aagtggcagg ataacttgat caacgccatc gccttactgg gcttttcaat
    15181 cccggtgttc tggctggcgc ttctgttgac gctgttttgt tcactcacgc taggttggtt
    15241 gcccgtttca gggcgtttcg atctgctcta cgaagtgaaa ccgattaccg gttttgcgtt
    15301 gattgatgcc tggctttcgg actcaccatg gcgggatgaa atgatcatga gcgcaatccg
    15361 ccatatgata ttgcccgtga tcaccctgtc ggttgcgcca acaactgaag tgatccgcct
    15421 gatgcgtatc agcactatcg aagtgtatga ccagaactat gtaaaagcgg cggcgacccg
    15481 cggtttgtcg cgctttacta ttttgcgtcg ccacgttctg cataacgcgt taccgccggt
    15541 tattcctcgc ctgggcttac agttttccac catgctgacg ctggcgatga tcaccgaaat
    15601 ggtttttagc tggccgggtt tagggcgctg gttaattaac gccatccgcc agcaggatta
    15661 cgcagccatt tccgccggag tgatggtgtg tggctcactg gtcattattg ttaacgtgat
    15721 ttctgatatt ttgggtgcca tggctaaccc tctgaaacat aaggaatggt atgccttacg
    15781 atagcgtata cagcgaaaag cgcccgccgg gcacgctgcg taccgcctgg cgcaaatttt
    15841 atagtgacgc ctctgcgatg gtcggcctgt acggctgcgc ggggctggct gtactgtgta
    15901 tttttggcgg ctggtttgcg ccttacggca tcgaccagca atttctcggt tatcaattat
    15961 tgccgccatc atggtcgcgc tatggcgaag tttctttctt cctggggact gacgacctcg
    16021 gacgcgatgt tttaagccgt ttactgagcg gagctgcgcc aaccgtgggt ggcgcatttg
    16081 tggtgacgct tgccgcgacg atctgtggcc tagtactggg caccttcgcc ggggcgacac
    16141 acggcctccg ctcagcggtg cttaaccata ttctggatac cttactggca atcccttcgc
    16201 tgctgttggc aattatcgtt gtagcgtttg ccggaccgag tttgtcccac gctatgcttg
    16261 ctgtctggct ggcattgctg ccgcgtatgg tacgttcgat ttacagcatg gtgcatgacg
    16321 aactggaaaa agagtacgtt atcgccgccc gtctggatgg cgcatcaacg ctgaatattc
    16381 tctggtttgc tgtgatgcca aacatcaccg ccgggctggt caccgagatc acccgcgcac
    16441 tgtcgatggc aattctcgat atcgccgcgc tgggctttct cgatctcggc gcacaactcc
    16501 cctcgcctga atggggagcg atgctcggtg atgcgctgga actgatttat gtcgccccgt
    16561 ggactgtcat gctgccaggt gcggcaatta tgattagcgt gttgttggtt aacctccttg
    16621 gcgatggcgt tcgtcgtgcg attattgcgg gggtggaata atgccattac tcgatattcg
    16681 taacctgacc attgaattta aaaccggtga tgagtgggtt aaagccgtcg accgggtaag
    16741 tatgacctta accgaaggtg aaatccgcgg tcttgttggt gaatccggtt ccggcaagag
    16801 tttgattgcg aaagcgattt gtggggtgaa taaagataac tggcgtgtta ctgctgaccg
    16861 tatgcgtttt gatgatatcg atttgctgcg tctctccgca cgcgaacggc gcaaactggt
    16921 tggccataac gtgtcgatga ttttccagga accgcagtcg tgtcttgacc cttcagaacg
    16981 tgtgggtcgc cagttgatgc aaaacatccc agcctggacc tataaaggcc gttggtggca
    17041 gcgttttggc tggcgcaaac gccgtgccat tgaactgctg caccgcgtgg ggattaaaga
    17101 tcacaaagat gcgatgcgca gttttcccta tgagttgacc gaaggtgaat gtcagaaagt
    17161 gatgatagcc attgcactgg cgaatcaacc gcgtctgctg attgctgacg aaccgaccaa
    17221 ctcaatggag ccaacaaccc aggcgcaaat ctttcgcctg ctgacgcgtc tcaaccaaaa
    17281 cagcaatacc actattttgc ttatcagcca tgacttacaa atgcttagcc aatgggcgga
    17341 taaaattaac gtgctttact gcggtcaaac agtggaaacc gcgccgagta aggagttggt
    17401 gacgatgcca catcatcctt atacccaggc gctgatccgc gcgataccag acttcggcag
    17461 cgcgatgccg cataaaagtc gcctcaatac gctgcccggc gctatcccac tgctggaaca
    17521 gttaccgatt ggttgtcgtc tggggccacg ttgcccgtat gcacaacgag aatgcattgt
    17581 gacgccacgt ttgacggggg cgaaaaatca tctctatgcc tgtcatttcc cgctgaacat
    17641 ggagaaagag tgagatgatc gaaacgctgc ttgaagtgcg taatttaagt aaaacgttcc
    17701 gctaccggac cggctggttt cgccgtcaga ccgtagaagc ggtaaaaccc ttgagcttta
    17761 cgctacgtga aggccagaca ctggcgatta ttggcgagaa tggttcgggt aaatccacgc
    17821 tggcaaaaat gctggcggga atgatagagc ctaccagcgg cgagttattg attgacgatc
    17881 atccactgca ttttggcgat tattccttcc gcagtcagcg tattcgcatg atttttcagg
    17941 atccttcgac ctcgttgaat ccccgtcaac gtatctcgca aattctggat tttccactgc
    18001 gcctgaacac cgacctggaa ccggaacagc gtcgcaaaca gattattgaa acgatgcgta
    18061 tggttgggct gctaccggat cacgtcagtt actatccgca tatgttggca cccggacaaa
    18121 aacagcgtct gggtctggcg cgcgcgctga tattgcgccc aaaagtcatt attgccgatg
    18181 aagcactcgc ttcactggat atgtcgatgc gttcgcagtt gattaatctg atgctggagt
    18241 tacaggaaaa acagggcatt tcgtatattt atgttaccca gcatatcgga atgatgaagc
    18301 acattagcga ccaggtgctg gtgatgcatc agggcgaggt tgtcgagcga ggcagcaccg
    18361 cggatgtgct ggcatcgccg ctgcatgaac tcaccaaacg gctgattgcc gggcactttg
    18421 gcgaagcatt gacagcggat gcgtggcgta aagatcgcta attttctgaa tcctccttcc
    18481 agattatcca cctcattctg acaagagatt aaatcttgtc agaatgaggt gatggataaa
    18541 ataaaatcaa atgcgcgtga tttaagacgc aatctcactt tgcaggaacg aaagctctgg
    18601 cgatatcttc gcagccgacg ttttggtgat ttcaaatttc gccgtcaaca tccggtgggg
    18661 agctacattc tcgattttgc ttgctgctcg gcgcgcgtag tcgttgagct ggatggtggg
    18721 cagcatgatt tggcagttgc ctatgatacc aggcgcacta gctggcttga gtcgcagggc
    18781 tggaccgtgc tgcgtttctg gaataacgag attgattgta atgaggaggc ggtgctggag
    18841 attattctgc aggaactgaa ccgccgatca ccctcaccct gaaagggcga gggggccgac
    18901 cgagccgaat agctgttgtg gtgaaaacat ggagacagtg ctggagaata ttcggcaagg
    18961 tctgaaccat cccagccatc gccataaaag ggttaggggc tgtatgagcc tgtttgttgc
    19021 tggggtaaca atatttgcac aatatggtcc cctcgcccct ctggggagag ggttagtgta
    19081 aggggaaaag cgcccaccct gccgcagcct gctccggtcg gacctagcaa ctatagctac
    19141 tcacagccag gttgattata ataaccattt atctgttcgt actgtttact aaaacgacga
    19201 atcgcctgat tttcaggcac aacaagcatc aacaataagg attaaagcta tgggttttct
    19261 ttccggtaag cgcattctgg taaccggtgt tgccagcaaa ctatccatcg cctacggtat
    19321 cgctcaggcg atgcaccgcg aaggagctga actggcattc acctaccaga acgacaaact
    19381 gaaaggccgc gtagaagaat ttgccgctca attgggttct gacatcgttc tgcagtgcga
    19441 tgttgcagaa gatgccagca tcgacaccat gttcgctgaa ctggggaaag tttggccgaa
    19501 atttgacggt ttcgtacact ctattggttt tgcacctggc gatcagctgg atggtgacta
    19561 tgttaacgcc gttacccgtg aaggcttcaa aattgcccac gacatcagct cctacagctt
    19621 cgttgcaatg gcaaaagctt gccgctccat gctgaatccg ggttctgccc tgctgaccct
    19681 ttcctacctt ggcgctgagc gcgctatccc gaactacaac gttatgggtc tggcaaaagc
    19741 gtctctggaa gcgaacgtgc gctatatggc gaacgcgatg ggtccggaag gtgtgcgtgt
    19801 taacgccatc tctgctggtc cgatccgtac tctagcggct tccggtatca aagacttccg
    19861 caaaatgctg gctcattgcg aagccgttac cccgattcgc cgtaccgtta ctattgaaga
    19921 tgtgggtaac tccgcggcat tcctgtgctc cgatctctct gccggtatct ccggtgaagt
    19981 ggtccacgtt gacggcggtt tcagcatcgc tgcaatgaac gaactcgaat tgaaataatc
    20041 attctgttgg taaatattgg gcggcgttct gccgcccgtt atctctgtta tacctttctg
    20101 atatttgtta tcgccgatcc gtctttctcc ccttcccgcc ttgcgtcagg ataacgattt
    20161 cctttacgac caaggagcgc ccatggaaca acgccacatc accggcaaaa gccactggta
    20221 tcatgaaacg caatccagta ctgcggagta tgacgttctg cctctggtcc cggaagccgc
    20281 aaaggtcagc gatccctttc tgctcgacgt gatccttgat gaagaaacgc tagctccctt
    20341 cctttcatgg ctggtccctg cgcgcgttct tgcagtggaa ttgttccctg accagcttac
    20401 cgtgacccgt tcacagacct tcaccgctta tgaacgcttg tctacggccc tgacggttgc
    20461 tcaggtttgc ggcgtccagc ggttatgtaa ctactattcg gcgcgactta cgccgctccc
    20521 cgggcctgat tcctctaggg aaagcaatca taggttggca caaatcacgc aatatgcccg
    20581 ccaactggct agctcgcctt ctattatcga caaccgatcg cgccagcatc tgaatgacgt
    20641 cggtcttact gccagggact gtgtaatcat taaccaaatc attggtttta ttggctttca
    20701 ggcgcggacc attgcgacat ttcaggctta tctcgggcac ccggtacgct ggttacccgg
    20761 tctggaaata caaaactacg ccgacgcgtc actgtttgct gatgaatcaa tacgctggcg
    20821 aagcagctat gaggtggaaa aactacctga agagtacaca aaaagttcaa cagcagaact
    20881 ttgccaactg gccgaaacac tctctctcca ccctatttca ctttcccttc tcgaaaggtt
    20941 gttaaacagt acacgggtta atacacagcc ggataataag cttgcggcgt tgttatgcgc
    21001 gcggataaat ggcagtcctg cttgttttgc cgcctgtatg gattcatcaa atgaatataa
    21061 aaaaatcagc ccccttctgc gcaagggcga aaatgaaatt aaccaatggg ctgaccgtca
    21121 ttctgttgag cacgctaccg ttcaggcgat acaatggctg acccgagcac ccgatcgctt
    21181 tagcaccgcc cagttcagcc ctttactcga acacgaacaa tcatcaacgc agattattaa
    21241 tctgctggta tggagcgggc tgtgtggctg gataaatcgc ttaaaaatcg cgttgggtga
    21301 gacatattaa ccttgccgcg tcagacagat tcgcgtaaaa ctgtcagccg ctctaatggc
    21361 caccaaaata gacaattatg tttcaggaca acccgctgct agcgcagctt aaacagcaac
    21421 tgcattccca gacgccacgc gctgaagggg tggtaaaagc cacagaaaaa ggctttggct
    21481 tcctggaagt cgacgcgcaa aaaagttatt tcattccgcc gccgcagatg aaaaaagtca
    21541 tgcatggcga ccgaattatc gcggtgatcc acagtgaaaa agaacgtgaa tccgcagagc
    21601 cagaagaact ggttgaaccg ttcctgactc gtttcgtggg taaagttcag ggcaaaaatg
    21661 accgtctggc catcgttcct gatcatccac tcttaaaaga cgccattcct tgccgcgcag
    21721 cccgtggcct gaaccacgag tttaaagaag gcgactgggc ggttgccgaa atgcgccgtc
    21781 atccgctgaa aggcgatcgt tctttctatg ctgaattgac acaatacatc acttttggtg
    21841 acgaccactt tgtaccgtgg tgggttacac ttgcgcgcca taatctggaa aaagaagcac
    21901 cagacggcgt cgctaccgaa atgctcgatg aaggtctggt tcgtgaagat ctgaccgcgc
    21961 tggattttgt caccatcgac agtgccagca cagaagatat ggatgacgcc cttttcgcta
    22021 aggcgttgcc ggatgacaaa cttcagctga ttgtggcgat tgccgatcca accgcgtgga
    22081 ttgctgaagg cagcaagctg gacaaagccg cgaaaattcg cgcattcacc aactatctgc
    22141 ctggcttcaa catccctatg ctgcctcgcg agctttctga cgatctctgc tcactgcgcg
    22201 ccaatgaagt ccgcccggta ctggcatgcc gcatgacgct ctccgctgat ggcgccattg
    22261 aagataatat cgaattcttt gccgccacca tcgaatccaa agcaaagctg gtgtatgacc
    22321 aggtttctga ctggctggaa aataccggtg actggcagcc tgaaagtgaa gcaattgccg
    22381 aacaagtccg tttgctagcg caaatttgcc aacgccgcgg cgagtggcgt cataaccacg
    22441 cactggtgtt taaagatcgc ccggattacc gctttattct cggtgaaaaa ggtgaagtgc
    22501 tggatatcgt cgccgagcct cgtcgcattg ccaaccgtat cgtcgaagaa gcgatgattg
    22561 ccgctaacat ttgtgcggcc cgcgtactgc gcgataagct cggttttggc atctataacg
    22621 tgcatatggg ctttgatccg gcgaatgccg acgcgctggc agcgttgctg aaaacgcacg
    22681 gtctgcatgt cgatgccgaa gaagtgctca cgctggacgg tttctgcaaa ctgcgtcgtg
    22741 aactggacgc gcaaccaact ggtttcctcg acagccgcat tcgtcgcttc cagtcatttg
    22801 ctgaaattag cactgaaccc ggtcctcact ttggcctcgg tctggaagca tacgccacct
    22861 ggacttcgcc gatccgtaaa tatggcgaca tgatcaacca ccgtctgctg aaagcggtta
    22921 tcaaaggcga aactgcgacg cgcccacagg atgaaatcac tgtccaaatg gccgagcgtc
    22981 gccgtctcaa ccggatggca gaacgtgatg ttggtgactg gttatacgca cgcttcctga
    23041 aagacaaagc cgggaccgac acccgtttcg cagcggaaat tgtcgatatc agccgtggcg
    23101 gcatgcgtgt gcgtttggtt gataacggcg ctatcgcctt tattccggca cctttcttac
    23161 acgctatgcg cgatgaactg gtttgcagcc aggaaaacgg cactgtacaa attaaaggtg
    23221 aaacggttta caaagtaact gacgttattg acgtcaccat tgccgaagtc cgcatggaaa
    23281 cccgcagcat tattgcgcgc ccggtcgcgt aatctccttt cacggcctat tcctcatgga
    23341 tgggccgttt atttccccgc tctaccttcg ttatatccag gcagattttt taactgactt
    23401 tcgtttgaaa actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaacaat
    23461 gcgtttagcc gccgttaatt tc
//
LOCUS       DAJTSL010000054        23030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTSL010000054 DAJTSL010000000
VERSION     DAJTSL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.054"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..562)
                     /locus_tag="OO433_001893"
     CDS             complement(<1..562)
                     /locus_tag="OO433_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6050391.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGG"
     gene            complement(781..1758)
                     /gene="epmA"
                     /locus_tag="OO433_001894"
     CDS             complement(781..1758)
                     /gene="epmA"
                     /locus_tag="OO433_001894"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6050392.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2083..3891
                     /gene="frdA"
                     /locus_tag="OO433_001895"
     CDS             2083..3891
                     /gene="frdA"
                     /locus_tag="OO433_001895"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6050393.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3884..4618
                     /gene="frdB"
                     /locus_tag="OO433_001896"
     CDS             3884..4618
                     /gene="frdB"
                     /locus_tag="OO433_001896"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6050394.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4629..5024
                     /gene="frdC"
                     /locus_tag="OO433_001897"
     CDS             4629..5024
                     /gene="frdC"
                     /locus_tag="OO433_001897"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6050395.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5035..5394
                     /gene="frdD"
                     /locus_tag="OO433_001898"
     CDS             5035..5394
                     /gene="frdD"
                     /locus_tag="OO433_001898"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6050396.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5457..6590
                     /locus_tag="OO433_001899"
     CDS             5457..6590
                     /locus_tag="OO433_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6050397.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6679..7212
                     /gene="blc"
                     /locus_tag="OO433_001900"
     CDS             6679..7212
                     /gene="blc"
                     /locus_tag="OO433_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6050398.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7209..7526)
                     /gene="sugE"
                     /locus_tag="OO433_001901"
     CDS             complement(7209..7526)
                     /gene="sugE"
                     /locus_tag="OO433_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6050399.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7708..7854)
                     /gene="ecnB"
                     /locus_tag="OO433_001902"
     CDS             complement(7708..7854)
                     /gene="ecnB"
                     /locus_tag="OO433_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6050400.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7965..8090)
                     /gene="ecnA"
                     /locus_tag="OO433_001903"
     CDS             complement(7965..8090)
                     /gene="ecnA"
                     /locus_tag="OO433_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6050401.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8142..8708)
                     /gene="efp"
                     /locus_tag="OO433_001904"
     CDS             complement(8142..8708)
                     /gene="efp"
                     /locus_tag="OO433_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6050402.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8750..9799
                     /gene="epmB"
                     /locus_tag="OO433_001905"
     CDS             8750..9799
                     /gene="epmB"
                     /locus_tag="OO433_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6050403.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            10173..11042
                     /locus_tag="OO433_001906"
     CDS             10173..11042
                     /locus_tag="OO433_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR6050404.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(11245..11598)
                     /locus_tag="OO433_001907"
     CDS             complement(11245..11598)
                     /locus_tag="OO433_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6050405.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11736..13382)
                     /gene="groL"
                     /locus_tag="OO433_001908"
     CDS             complement(11736..13382)
                     /gene="groL"
                     /locus_tag="OO433_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6050406.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13426..13719)
                     /locus_tag="OO433_001909"
     CDS             complement(13426..13719)
                     /locus_tag="OO433_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6050407.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13995..15251
                     /gene="yjeH"
                     /locus_tag="OO433_001910"
     CDS             13995..15251
                     /gene="yjeH"
                     /locus_tag="OO433_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6050408.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPCAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRSASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIVLAGLWLLLPKRKTPENGIIT"
     gene            complement(15267..15743)
                     /gene="fxsA"
                     /locus_tag="OO433_001911"
     CDS             complement(15267..15743)
                     /gene="fxsA"
                     /locus_tag="OO433_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6050409.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            16080..17516
                     /gene="aspA"
                     /locus_tag="OO433_001912"
     CDS             16080..17516
                     /gene="aspA"
                     /locus_tag="OO433_001912"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6050410.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17634..18935
                     /gene="dcuA"
                     /locus_tag="OO433_001913"
     CDS             17634..18935
                     /gene="dcuA"
                     /locus_tag="OO433_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6050411.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            19051..19389
                     /gene="cutA"
                     /locus_tag="OO433_001914"
     CDS             19051..19389
                     /gene="cutA"
                     /locus_tag="OO433_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6050412.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19365..21062
                     /gene="dsbD"
                     /locus_tag="OO433_001915"
     CDS             19365..21062
                     /gene="dsbD"
                     /locus_tag="OO433_001915"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6050413.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNHALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NITANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            21099..21674
                     /locus_tag="OO433_001916"
     CDS             21099..21674
                     /locus_tag="OO433_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6050414.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKQLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21781..21856
                     /locus_tag="OO433_001917"
     tRNA            21781..21856
                     /locus_tag="OO433_001917"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21814..21816,aa:Phe,seq:gaa)
     gene            22054..>23030
                     /locus_tag="OO433_001918"
     CDS             22054..>23030
                     /locus_tag="OO433_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6050415.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGTGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQTVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENVMNAS"
BASE COUNT         5738 a         5945 c         5700 g         5647 t
ORIGIN      
        1 gcccaccatt taataacaaa atagtaatgc ttactaacag caatactaaa ttcagacaca
       61 ttactgcaat accgcccacc gcagtaatgc gggcaatttt attaatcccc tttgaagcaa
      121 cgacggtgac cagaatcatc catgctactg ccagcagacc aaccacctgc gtaggctcca
      181 gtccggcaat acgccagtgc tgggtcatgt cgctaccata gaggaatgtt gagaacggta
      241 cccaaacttt cgcggaggta ctcaccatcc agatgatata agaggaaaac cacataaacg
      301 taccaatgaa ggcaaaacgt ggtccgacac tattattcat ccaggaatag ataccacctt
      361 cttctttgcg ataagcagct cccatttcag ccatcattaa ggcgaatgga ataaagaata
      421 acaatgcaga aaatatataa aagggaatcg cactataacc cattaagtaa taagccgatg
      481 ggctattggc aaatccaaat acggaagtaa agatcatcaa tatgagtcct atcagactca
      541 tcttttttat cgtgtgaggc atgaaaccat ccttcatttc agcgcgtgta taacacgtgc
      601 ccactgaact gcactgcgca acattgagca ggcagcgtaa aaaccaatac agaattgata
      661 cccggatgat atcaaatatc cagtcttaaa ttgtggcctt taacggaaaa atgaaaacat
      721 atgctacaga atggcgctta tcacgccatt cttcgctgtt aattcagtaa tttttcagaa
      781 ttatgcccgg tcaacgctaa aggcgatgac ttcagccagt gtctccgcgc ccagcgccaa
      841 catcaccaga cgatcaacac ctaatgccac gccggaacag tcaggcatac cgactttcaa
      901 ggcttcaatc agattctggt caatggggtg ctgcggcaaa ccgcgcgccg cgcgcttacg
      961 gttatcttgt tcaaagcgtt gttgctgctc acgggcatcc gttaattcat ggaaaccatt
     1021 cgccagctca atacctttat aataaacctc aaagcgttca gcgacccgat gatcttcggt
     1081 actgatttgc gccagtgatg cctggctggc tggaaagtgg tacacaaagg tcggtttttc
     1141 tttgccaata tttggctcta cgccaaaggt aaacagcaat tgtagcaacg tgtcgcggtc
     1201 ttcttcggta tcagcaacgt tactcaaatc cagtttcgct gcgacttccc gcagttgcgt
     1261 tttgtcggca gagagcgggt caatttccag ataacgcaag aaagcttgtt gataagaaag
     1321 gctttctgct gccgggcagt ccagcacctg ttgtaagaga tcgtccacct cgttcatcaa
     1381 ccggtacata tcatagtgcg gtcgatacca ctcaagcata gtgaactcag ggttgtgata
     1441 acgccccatc tcttcattac ggaagctgcg gcacagctgg aataccggcc cacaaccggc
     1501 aaccagcagg cgtttcatat ggtattccgg gctggtcatt aaccagagat tcatcccctg
     1561 cgaatgcccg gggccaacga aacgtgtctc aaacgggacc aaatgaatat cggttaccgt
     1621 cgcctggctc atacaaggcg tctccacctc cagcactcca cgatcagcaa agaaacgacg
     1681 gatctccgcc ataatcgccg cgcgttttaa taagttagga atggatgcgc tcggctgcca
     1741 ggatgccgtt tcgctcatag ttaaatctcc agtttttgac aagggcacga agtctactcg
     1801 caacgcgacg gcgagacaaa ttttacgcag gaatcaaaca gcggtgggca gtgactaaaa
     1861 aaagcacgat ctgatggttt agtaattaaa ttaatcatct tcagtgataa tttagccctc
     1921 ttgcgcacta aaaaaatcga tctcgtcaaa tttcagactt atccatcaga ctatactgtt
     1981 gtacctataa aggagcagtg gaatagcgtt cgcagaccgt aactttcagg cacttaccct
     2041 gaagtacggg gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac ctttcaagcc
     2101 gatcttgcca ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc tgccgcgcag
     2161 gcaaatccaa atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg tagccatacc
     2221 gttgctgcag aagggggctc cgccgctgtc gcgcaggatc atgacagctt cgaatatcac
     2281 tttcacgata cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt ggattatttc
     2341 gtccaccact gcccaaccga aatgacccaa ctggaactgt gggggtgccc atggagccgt
     2401 cgcccggatg gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga gcgcacctgg
     2461 ttcgccgccg ataagaccgg cttccatatg ctgcacacgc tgttccagac ctctctgcaa
     2521 ttcccgcaga tccagcgttt tgacgaacat ttcgtgctgg atattctggt tgatgatggt
     2581 catgttcgcg gcctggtagc aatgaacatg atggaaggca cgctggtgca gatccgtgct
     2641 aacgcggtcg ttatggctac tggcggtgcg ggtcgcgttt atcgttacaa caccaacggc
     2701 ggcatcgtta ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc gctgcgtgac
     2761 atggaattcg ttcagtatca cccaaccggt ctgccaggtt ccggtatcct gatgaccgaa
     2821 ggctgccgtg gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta tctgcaagat
     2881 tacggcatgg gcccggaaac tccgctgggc gagccgaaaa acaaatatat ggaactgggt
     2941 ccacgcgaca aagtttctca ggccttctgg cacgaatggc gtaaaggcaa caccatctcc
     3001 acgccgcgtg gcgatgtggt ttatctcgac ttgcgtcacc tcggcgagaa aaaactgcat
     3061 gaacgtctgc cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga tccggttaaa
     3121 gaaccgattc cggtacgtcc gaccgcacac tacaccatgg gcggtatcga aaccgatcag
     3181 aactgtgaaa cccgcattaa aggtctgttc gccgtgggtg aatgttcctc tgttggtctg
     3241 cacggtgcaa accgtctggg ctccaactcc ctggcggaac tggtggtctt cggccgtatg
     3301 gccggtgaac aagcgacaga gcgtgcagca actgccggta atggcaacga agaggcaatt
     3361 gaagcgcagg cagctggcgt tgaacaacgt ctgaaagatc tggttaacca ggatggcggc
     3421 gaaaactggg cgaagatccg cgacgaaatg ggcctagcaa tggaagaagg ttgcggtatc
     3481 taccgtacgc cggaactgat gcagaaaacc atcgacaagc tggcagagct gcaggaacgc
     3541 ttcaagcgcg tgcgcatcac cgacacctcc agcgtgttca acaccgacct gctctacacc
     3601 attgaactgg gccacggtct gaacgttgct gaatgtatgg cgcactccgc aatggcacgt
     3661 aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg tgacgacgtc
     3721 aacttcctca aacacaccct cgccttccgc gatgctgatg gcacgactcg cctggagtac
     3781 agcgacgtga agattactac gctgccgcca gctaaacgcg tttacggtgg cgaagcggat
     3841 gcagccgata aggcggaagc agccaataag aaggagaagg cgaatggctg agatgaaaaa
     3901 cctgaaaatt gaggtggtgc gctataaccc ggaagtcgat accgcaccgc atagcgcatt
     3961 ctatgaagtg ccttatgacg caactacctc attactggat gcgctgggct acatcaaaga
     4021 caacctggca ccggacctga gttaccgctg gtcctgccgt atggcgattt gtggttcctg
     4081 cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa accttcctgc gtgattacac
     4141 cgacggtatg aaggttgaag cgttagctaa cttcccgatt gaacgcgatc tggtggtcga
     4201 tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg gcaactcccg
     4261 caccgcggat cagggtacta acatccagac cccggcgcag atggcaaagt atcaccagtt
     4321 ctccggttgc atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc
     4381 agagttcatc ggtccggctg ccattacgct ggcgcatcgt tataacgaag atagccgcga
     4441 ccacggtaag aaggagcgta tggcgcagtt gaacagccag aacggcgtat ggagctgtac
     4501 tttcgtgggc tattgctccg aagtctgccc gaaacacgtc gatccggctg cggccattca
     4561 gcagggcaaa gtagaaagtt cgaaagactt tcttatcgcg accctgaaac cacgctaagg
     4621 agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga
     4681 aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt
     4741 ggttcagcat tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg gacgcctggg
     4801 cgggattcgt cgacttttta caaaacccgg ttatcgtgat cattaacctg atcactctgg
     4861 cggcagctct gctgcacacc aaaacctggt ttgaactggc accgaaagcg gccaatatca
     4921 ttgtaaaaga cgaaaaaatg ggaccagagc caattatcaa aagtctctgg gcggtaactg
     4981 tggttgccac catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt
     5041 aatccaaatc caaagcgttc tgacgaaccg gtattctggg gcctcttcgg ggccggtggt
     5101 atgtggagcg caattattgc gccggtgatg atcctgctgg tgggtattct gctgccactg
     5161 gggctgtttc cgggtgatgc gctgagctac gagcgcgttc tggcgttcgc gcagagcttc
     5221 attggtcgcg tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt
     5281 atgcaccacg cgatgcacga tctgaaaatt cacgtgcctg cgggcaaatg ggttttctac
     5341 ggtctggctg ctatcctgac agttgtcacg ctgattggta tcgttacaat ctaacgtatc
     5401 gccaatgtaa atccggcccg cctatggcgg gccgttttgt atggaaacca gaccttatgt
     5461 tcaaaacgac gctctgcgcc ttattaatta ccgcctcttg ctccacattt gctgcccctc
     5521 aacaaatcaa cgatattgtg catcgcacaa ttaccccgct tatagagcaa caaaagatcc
     5581 ccggtatggc ggtggcggta atttatcagg gtaaacctta ttactttacc tggggctatg
     5641 cggacatcgc caaaaagcag cccgtcacac agcaaacgtt gtttgagtta ggttcggtca
     5701 gcaaaacatt tacgggcgtg cttggtggcg atgctattgc tcgaggggaa atcaagttaa
     5761 gcgatcccgc aacaaaatac tggcctgaac ttaccgctaa acagtggaat gggatcacac
     5821 tattacatct tgcgacctac accgctggcg gcctgccatt gcagatgccg gatgaagtga
     5881 aatcctcaag cgacttgctg cgcttctatc aaaactggca gcctgcatgg gctccaggaa
     5941 cacaacgtct gtatgccaac tccagtatcg gtttgttcgg cgcactggct gtgaagccgt
     6001 ctggtttgag ttttgagcag gcgatgcaaa ctcgtgtctt ccagccactc aaactcaacc
     6061 atacgtggat taatgtaccg cccgcagaag aaaagaatta cgcctgggga tatcgcgaag
     6121 gcaaggcagt tcatgtttcg ccaggggcgt tagatgctga agcttatggt gtgaagtcga
     6181 ccattgaaga tatggcccgc tgggtgcaaa gcaatttaac cccccttgat atcaatgaga
     6241 aaacgcttca acaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt
     6301 atcagggcct gggctgggaa atgctggact ggccggtcaa tcctgacagc atcattaacg
     6361 gcagtgacaa taaaattgca ctggcagcac gcccggtaaa agcgattaag cccccaactc
     6421 ctgcagtacg cgcatcatgg gtacataaaa cgggggcgac cggcggattt ggtagctatg
     6481 tcgcgtttat tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa aactatccca
     6541 atccagcgag agtcgccgcc gcctgtcaga ttctcaacgc tctacagtaa aatttcatcg
     6601 ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa
     6661 accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt
     6721 ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg tgacagtggt gaataatttc
     6781 gacgctaaac gctatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa
     6841 cgtggactgg aaaaagtcac cgcaacatac agtctgcgtg atggcggcgg cctgaatgtc
     6901 atcaataaag gctataaccc tgacagagaa atgtggcagc agagtgaagg gaaagcgtac
     6961 tttaccggcg caccaacacg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc
     7021 ggttataacg tcattgcact tgatcgggaa taccgccatg cgctggtttg cggcccggac
     7081 cgcgactacc tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag
     7141 atgctggcag tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag
     7201 cccggtagtt agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga
     7261 ctcgccaggc gcatcgggtt agcggactca ccgagcagca caatgccggt gatagccgcg
     7321 ccgactgcgc caatacccgt ccacacggca taagccgtcc ctactggtaa cgatttcatc
     7381 gcccaggcaa gtagcgccat actgacaatc atcgccgtca cggtaataac actcggcgtc
     7441 aaacgactaa agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca
     7501 gcaataacta agataatcca ggacatatca ggctccggag aattggggcc gtccccggtg
     7561 aaaagacgcg tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgccaggt
     7621 ggcaggggaa tttcaactat taccttagtg actatcgcag aaaaaacaaa cggcacgaca
     7681 cagctgtcgt accgttattg cttattgcta ttgctgcgct ttcgttgctg cgccagaaat
     7741 cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa
     7801 tactgttgaa agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc
     7861 ttccttttag gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc
     7921 caaaaatgac ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga
     7981 gagatggagt tgccgagatg tttgatgtct tcgccgaaac cgcgagcggt gttacagccc
     8041 gtgagcagcg tactggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg
     8101 aataagtgat ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg
     8161 tattcaccag agcgggtatc cactttgatg acttcgccga tttgtacgaa cagcggaacc
     8221 ttaaccacag cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct
     8281 ttcaggcccg gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag
     8341 atcggctgac cattccacag agttacgata cactctgcct gatccagcag ccatttagcg
     8401 ttatcgccaa ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag
     8461 tgccagaact caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct
     8521 tcagcggaat cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc
     8581 agtttaacgc gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc
     8641 gcgtaaggtt cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac
     8701 gttgccataa ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat
     8761 tgtaacccta aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac
     8821 cgatcctgat gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg
     8881 acgcagcgcc aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga
     8941 gaaaggcaat ccggacgatc ctcttttgcg tcaggtactt acctcgcaag atgagtttgt
     9001 cgtcgcgtcc ggattctcca ccgacccact ggaagaacag cacagcgtag tgcctggttt
     9061 gttgcataaa taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg
     9121 ccgctattgc ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg
     9181 gcagactgca cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg
     9241 cggcgatccg ctgatggcga aagatcacga gctggactgg ttgctcacac aactggaagc
     9301 catcccgcat atcaaacgtc tgcggattca cagccgtctg ccgattgtga tcccggcacg
     9361 catcacagat gcgctggttg aacgcttttc acattctacg ctgcaaatcc tgctggtgaa
     9421 tcacatcaac catgccaatg agatagatga aacattccgt caggcgatgg ctaagttgcg
     9481 ccgggtaggc gttactttgc tgaaccagag cgttctgtta cgtgatgtga acgataacgc
     9541 acaaacgctg gcaaacctga gtaatgcgtt gttcgatgcc ggcgtgatgc cgtattacct
     9601 gcatgtgctc gataaagtac agggcgcagc gcattttatg gtgagtgatg acgaagcacg
     9661 gcagattatg cgtgagttgc tgacattggt gtcgggatat ctggtgccga aactggcgcg
     9721 agaaattggc ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc agcagttact
     9781 gattaaacag ggaagttaaa cattaaaatt gcctgatgcg ctacgcttat caggcctaca
     9841 tcgtgcattg aaattgaatg gatttgcaat gaactgtagg ccggataagg cgtttacgcc
     9901 gcatccggca agagcaacaa acgcaagtac agcaacctgt cattaaactg agaaatttaa
     9961 tctttgcccg ttgcataacc tttaattatt catatgcatt gctgttgtta ctttttatat
    10021 agctaacacc aataattaac ctttcttttc tgtttatata gagtgagcac ggctatttat
    10081 gttattttaa caactattac taccaagccc atttatctgg cttataaatt aaaattaatt
    10141 atttaattga gaaatggtta gggagaacct acatggccat aagcatcaag ggtgttaata
    10201 ccggtgttat tcgcaagagt aataatttca ttgcactggc gctgaaaatt aaagaacctc
    10261 gcaataaaga atcgttgttc tttatgtctg tcatggaact gcgcgacctg ctgattgcgc
    10321 tggaaagtcg tctgcatcaa aagcataaac tggatgctgc tgcacgcctt cagtatgagc
    10381 aagctcgcga taaagtcatt aagaaaatgg cagaaaacat cccagaaatt ctggttgatg
    10441 agcttaaaaa tgccgatatt aatcgtcggg ttaacacgct ggaacttacc gataatcaag
    10501 gcgaaaatct taccttcgta ttaacgctac atgacggtag caaatgtgag ttagtcgtca
    10561 atgagttgca gattgaaatg ctagcgcgag caatcattca tgcaattaat aatgcggaaa
    10621 tgcgtgaact ggcattacgt attacctcac tgttagattt cctgccgtta tatgacgttg
    10681 actgccagga aaatggcaat ctggaatatg acacctattc ccaaccggaa tggaaacata
    10741 acttgttcga ccattatctg gcggtgcttt accgctttaa agatgaaagt ggcaaagaac
    10801 aatttagcgg cgcagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa gtagaggcga
    10861 tcactcgccg aatgcttgat ttcagcccac gactgaaaaa actcgcgggt gtgccatgtc
    10921 aggtctatgt tcgcaccgta gcggcgaata acgcccaacc gctaacccag gatcaatgct
    10981 tacgcgcgct gcatcactta cgtgttcaat ccaccagcaa aactgcacca caggcgaaat
    11041 aatctcatct ttgctaatgc gaggctggag ccatccagcc tgaaatgact gataacgtga
    11101 tcgttggtca tgccggatgc ggcgtaaacg ccttatccgg cctacaaatt ctcaagcatt
    11161 caatcagttg caatgcctgc tgtaggcctg ataagcgaag cgcatcaggc aataatacgt
    11221 ttgtcaccca gcctctggat cacatcagtt cgggcactta taaacctgac cgataatctg
    11281 gctatccgtc gggacaaaac tggacaacat tccctgcgac gggctgctga tgccataaat
    11341 cacgttaccg cccattgcag ccgcctggtt gcgcagatcg tttgctgcgc cgcgcataga
    11401 accgccctct tctccgtgtt gcccggaaag ccagttgctt tgcttacctg tcgcagtacc
    11461 aatcagctgg cactctgcgc ctggctgctc gtccacaatg cgtacactct gaccggcagc
    11521 actcaattcg ttgctggagc tacaacccgc catcagtagc gcggcaccga caatccctgc
    11581 taagtatttt acgtgcatgt tattccccat aatcaatgag ctggacgcca ctcgcgtcgt
    11641 ccgtgtctga atcttatact agaaagatga ccaaaagaaa aacccccaga catttctgcc
    11701 cgggggtttg tttatttctg cgaggtgcag ggcaattaca tcatgccgcc catgccaccc
    11761 atgccgccca taccgccagc agcgcctaag tcagctgcat cgtttttcgg caggtcggta
    11821 accatgcact cggtggtgat catcaggcca gccacagaag ccgcgtactg cagagcagaa
    11881 cgggttactt tggttggatc caagataccc atgtcgatca tgttgccgta ttcttcggtt
    11941 gctgcgttgt aaccgtagtt gccgtcgccg cctttaacag tgttagcaac aacagacggt
    12001 tcttcgccgc agttcaatac gatctgacgc agcggagctt ccattgcacg cagtgcaact
    12061 ttgataccca cgttctggtc ttcgttctga ccacgcaggt cagccagttt agacgctacg
    12121 cggatcagcg caacaccacc accagcaacc acgccttctt ctaccgcagc acgggtcgcg
    12181 tgcagggcat cttcaacgcg tgcttttttc tctttcattt caacttcggt agcagcacct
    12241 actttgataa ctgcaacgcc gcctgccagt ttcgctacgc gctcctgcag tttttcacgg
    12301 tcgtagtcag aagttgcttc ttcaatctgc tgacggatct gagcaacacg gccctggatt
    12361 gcagcttctt cacccacgcc atcaatgatg gtggtggtgt ctttgttgat cacaacacgt
    12421 ttagcctgac ccaggtcttc cagggttgct ttttccagct ccataccgat ctcttcagag
    12481 attacggtac cgccagtcag ggttgcgata tcctgcagca tagctttacg gcgatcgccg
    12541 aagcccggag ctttaaccgc agcaactttc acgatgccac gcatggtgtt aacaaccaga
    12601 gttgccagcg cttcgccttc aacatcttca gcgatgatca gcagcggttt acctgcttta
    12661 gcaacggctt ccagaaccgg cagcatttcg cggatgttgg agattttctt gtcagccagc
    12721 aggatgaacg ggctttccag ttctactgcg ccagtttccg gcttgttgat gaagtaagga
    12781 gacaggtagc cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc ctgcagaccg
    12841 gtaccgtctt caacggtgat aacgccttct ttaccgactt tgtccatcgc ttcagcgatc
    12901 agtttaccta cggtttcgtc ggagttagcg gagatggtac caacctgagc aatcgcttta
    12961 gagtcagagc atggtacgga cagcgctttc agttcttcaa ctgcagcggt aacagctttg
    13021 tcgataccac gtttcaggtc catcgggttc atgcccgcag caactgcttt cagaccctca
    13081 gtgatgatag cctgagccag tacggttgca gtggtggtac cgtcgcctgc agcgtcgttc
    13141 gctttagagg caacttcttt caccatctgc gcacccatat tttcgaactt gtcttccagt
    13201 tcgatttcac gagcaacgga aacaccatct ttggtgatgg tcggtgcacc gaaagattta
    13261 tccagaacta cgttacggcc tttcggaccg agggtaactt tcactgcatc tgccagtacg
    13321 tttacgccgc gcagcatttt cacacgagcg tcgttaccga attttacgtc tttagctgcc
    13381 attatcttta ttccttaaat tcgtatgttc agtgtcgtgc gaggattacg cttcaacaat
    13441 tgccagaatg tcgctttcgg acatgatcaa cacttcttca ttgtcgatct tctcagattt
    13501 cacaccgtag ccatcgttga aaataacgat gtcgccaact ttcacatcca gcggcttcac
    13561 ttcgccattt tcaaggatac ggccattgcc gacagccagc acttcgccgc gggtggattt
    13621 agccgctgca gagccggtca gaacgatgcc gccagcagat ttagtttcaa cttctttacg
    13681 cttgacgatc acgcgatcat gcaatggacg aatattcatt gataactctc ctttgagaaa
    13741 gtccgtatct gttatgggtg acgccggagc ttacgtggtt tcccggctgg tgaccagaga
    13801 aatggggatg aggattcgcc ccttcaaggg ggaaaaagaa aaaaaattct gatcagcaca
    13861 aaatcgggtg aaaaccctga ttcacctcac atttcatcgc aatttcttca tcgccgtcat
    13921 aagcgaatct gattgtgcta ccatcgaaaa tctacgcagt tgcccaaaat ttgggcgcaa
    13981 tcggatatca acccatgagt ggactcaaac aagaactggg gctggcccag ggcattggcc
    14041 tgctatcgac gtcattatta ggcaccggcg tgtttgccgt tcctgcgtta gctgcacagg
    14101 tagcaggcaa taacagcctg tgggcgtggc ccgttttgat tatcttagtg ttcccgattg
    14161 cgattgtgtt tgcgattctg ggtcgccact atccctgcgc aggcggtgtc gcgcacttcg
    14221 tcggtatggc gtttggttcg cggcttgagc gagtcaccgg ctggttgttt ttatcggtca
    14281 ttcccgtggg tttgccagcc gcgctacaaa ttgccgccgg gttcggccag gcaatgtttg
    14341 gctggcatag ctggcaactg ttgttggcag aactcggtac gctggcgctg gtgtggtata
    14401 tcggtactcg cagtgccagt tccagtgcta atctacaaac cgttattgcc ggacttatcg
    14461 tcgcgctgat tgtcgctatc tggtgggcgg gcgatatcaa acctgcgaat atccccttcc
    14521 cggcacctgg taatatcgaa cttaccgggt tatttgctgc gttatcagtg atgttctggt
    14581 gttttgtcgg tctggaagca tttgcccatc ttgcctcgga atttaaaaat ccagagcgtg
    14641 attttcctcg cgcattgatg attggtctgc tgctggcagg attagtctac tggggctgta
    14701 cggtagtcgt cttacacttc gacgcctatg gtgaaaaaat ggcggcggca gcatcgcttc
    14761 caaaaattgt agtgcaactg ttcggtgtag gagcgttatg gattgcctgc gtgattggct
    14821 atctggcctg ctttgccagt ctcaacattt atatacagag cttcgcccgc ctggtctggt
    14881 cgcaggcgca acataatcct gaccactacc tggcacgcct ctcttctcgc catatcccga
    14941 ataatgccct caatgcggtg ctcggctgct gtgtggtgag cactttggtg attcatgctt
    15001 tagagatcaa tctggacgct cttattattt atgccaatgg catctttatt atgatttatc
    15061 tgttatgcat gctggcaggc tgtaaattat tgcaaggacg ttatcgacta ctggcggtgg
    15121 ttggcgggct gttatgcgtt ctgttactgg caatggtcgg ctggaaaagt ctctatgcgc
    15181 tgatcgtgct ggcggggtta tggctgttgc tgccaaaacg aaaaacgccg gaaaatggca
    15241 taatcacata atccggcgtt tcgacattaa tcctggcgat cgtctttatg atcaaggcgg
    15301 tcgcgctcat catcctttcg ctggtactca ccatcaaaag tattaccgcc accggtcccg
    15361 gcgctaaaac cgccgcctgg catacgagaa aagcgcaaat gcggcatcaa cttcactgtc
    15421 agatgctttt gcaccggcgg taataaaagc agaagaccga ggaagtcggt gaaaaagccc
    15481 ggtaataaaa gcagcaaacc ggcaatgatc agcgaaacac ttttaatcat ctccgccgct
    15541 gggttttcac ccgccgccat tttttgctgc atcagcacga aattcttaaa gccctggtta
    15601 cgtaccagtg acatcccgat aactgacgtg aatataacca gcacgagggt cagcaatacc
    15661 cccaatacat gggcaacctg aataaagatt gaaatctcaa tatagacata aaggaaaatg
    15721 gcaataaaag gtaaccagcg caaaggtttc tcctgtaata gcagccggta aaacccggct
    15781 acctgaatgg gttgcgaatc gcgttcagct tatattgtgg tcattagcaa aatttcaaga
    15841 tgtttgcgca actatttttg gtagtaatcc caaagcggtg atctatttca caaattaata
    15901 attaaggggt aaaaaccgac acttaaagtg atccagatta cggtagaaat cctcaagcag
    15961 catatgatct cgggtattcg gtcgatgcag gggataatcg tcggtcgaaa aacattcgaa
    16021 accacatata ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag aaggttcaca
    16081 tgtcaaacaa cattcgtatc gaagaagatc tgttgggtac cagggaagtt ccagctgatg
    16141 cctactatgg tgttcacact ctgagagcga ttgaaaactt ctatatcagc aacaacaaaa
    16201 tcagtgatat tcctgaattt gttcgcggta tggtaatggt taaaaaagcc gcagctatgg
    16261 caaacaaaga gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt gccgcatgtg
    16321 atgaagtcct gaacaacgga aaatgcatgg atcagttccc ggtagacgtc taccagggcg
    16381 gcgcaggtac ttccgtaaac atgaacacca acgaagtgct ggccaatatc ggtctggaac
    16441 tgatgggtca ccagaaaggt gaatatcagt acctgaaccc gaacgaccat gttaacaaat
    16501 gtcagtccac taacgacgcc tacccgaccg gtttccgtat cgcagtttac tcttccctga
    16561 ttaagctggt agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa gctgtcgaat
    16621 tccaggacat cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg atgaccctcg
    16681 gtcaggaatt ccgcgctttc agcatcctgc tgaaagaaga agtgaaaaat atccaacgta
    16741 ccgctgaact gctgctggaa gttaaccttg gcgcaacagc aatcggtact ggtctgaaca
    16801 cgccgaaaga gtactctccg ctggcagtga aaaaactggc tgaagtcact ggcttcccat
    16861 gcgtaccggc tgaagacctg atcgaagcga cctctgactg cggcgcttat gttatggttc
    16921 acggcgcgct gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac ctgcgcttgc
    16981 tctcttctgg cccacgtgcc ggcctgaacg agatcaacct gccggaactg caggcgggct
    17041 cttccatcat gccagctaaa gtaaacccgg ttgttccgga agtggttaac caggtatgct
    17101 tcaaagtcat cggtaacgac accactgtta ccatggcagc agaagcaggt cagctgcagt
    17161 tgaacgttat ggagccggtc attggccagg ccatgttcga atccgttcac attctgacca
    17221 acgcttgcta caacctgctg gaaaaatgca ttaacggcat cactgctaac aaagaagtgt
    17281 gcgaaggtta cgtttacaac tctatcggta tcgttactta cctgaacccg ttcatcggtc
    17341 accacaacgg tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt gtacgtgaag
    17401 tcgttctgga acgcggtctg ttgactgaag cggaacttga cgatattttc tccgtacaga
    17461 atctgatgca cccggcttac aaagcaaaac gctatactga tgaaagcgaa cagtaatcgt
    17521 acagggtagt acaaataaag aaggcacgtc agatgacgtg ccttttttct tgtgaacagt
    17581 aacttaaaaa caacaatcta atatcaactt gttaaaaaac aaggaaggct aatatgctag
    17641 ttgtagaact catcatagtt ttgctggcga tcttcttggg cgccagattg gggggaatag
    17701 gtattggttt tgcaggcgga ttgggggtgc tggttcttgc cgctattggc gttaaacccg
    17761 gtaacatccc gttcgatgtc atttccatta tcatggcggt tatcgccgct atttctgcca
    17821 tgcaggttgc tggcggtctg gactatctgg ttcatcagac agaaaagctg ctgcgccgta
    17881 acccgaaata catcacgatc ctcgcaccta tcgtgaccta tttcctgaca atctttgctg
    17941 gtactggcaa catctctctg gcgacactgc cagttatcgc tgaagttgcg aaggaacaag
    18001 gcgttaaacc ttgccgtccg ctctctactg cagtggtatc cgcgcagatt gcgatcaccg
    18061 catcgccaat ctcagcggca gtggtttaca tgtcttccgt gatggaaggt catggcatca
    18121 gctacctcca tctgctctcc gtggtcatcc cgtccaccct gctggcggtt ctggtgatgt
    18181 cctttctggt cactatgctg ttcaactcca aactctctga cgatccgatt tatcgcaagc
    18241 gtctggaaga gggcctggtt gaactgcgcg gtgaaaagca gattgaaatc aaatccggtg
    18301 caaaaacgtc cgtctggctg ttcctgctgg gcgtagttgg cgtggttatc tatgcaatca
    18361 tcaacagccc aagcatgggt ctggttgaaa aaccgctgat gaacaccacc aacgcaatcc
    18421 tgatcatcat gctcagcgtt gcaactctga ccaccgttat ctgtaaagtc gataccgaca
    18481 acatcctcaa ctccagcacc ttcaaagcag gtatgagcgc ctgtatttgt atcctgggtg
    18541 ttgcgtggct gggcgatact ttcgtttcca acaacatcga ctggatcaaa gataccgctg
    18601 gtgaagtgat tcagggtcat ccgtggctgc tggccgtcat cttcttcttt gcttctgctc
    18661 tgctgtactc tcaggctgca accgcaaaag cactgatgcc gatggctctg gcactgaacg
    18721 tttcaccgct gaccgctgtt gcttctttcg ctgcggtgtc tggtctgttc attctgccga
    18781 cctacccgac gctggttgct gcggtacaga tggatgacac gggtactacc cgtatcggta
    18841 aattcgtctt caaccatccg ttcttcatcc cgggtactct gggtgttgcc ctggccgttt
    18901 gcttcggctt cgtgctgggt agcttcatgc tgtaatgatc aatcgcgggg cgttcacgcc
    18961 ccgctttctt tcccgccgac taacatcctt cccccgtacg ttgtatagtg acctctctct
    19021 tgcggttcca tctgttcttg cgaggtgttt atgcttgatg aaaaaagttc gaataccgcg
    19081 tctgtcgtgg tgctatgtac ggcaccagat gaagcgacag cccaggattt agctgccaaa
    19141 gtgctggcgg aaaaactggc ggcctgcgcg accttgatcc ccggcgctac ctctctctat
    19201 tactgggaag gtaagctgga gcaagaatac gaagtgcaga tgattttaaa aactaccgta
    19261 tctcaccagc aggcactgct ggaatgcctg aagtctcatc atccatatca aaccccggaa
    19321 cttctggttt tacctgttac acacggagac acagattacc tctcatggct caacgcatct
    19381 ttacgctgat cctgctactt tgcagcactt ccgtttttgc cggattattc gacgcgccgg
    19441 gacgttcaca atttgtcccc gcggatcaag cctttacttt tgattttcag caaaaccaac
    19501 atgaccttaa tctgacctgg cagatcaaag acggttacta cctctaccgt aaacagatcc
    19561 gcattacgcc ggaacacgcg aaaattgccg acgtgcagct gccgcaaggc gtctggcatg
    19621 aagatgagtt ttacggcaaa agcgagattt accgcgatcg gctgacgctt cccgtaacca
    19681 tcaaccaggc gagtgcggga gcgacgttaa ctgtcaccta ccagggctgt gctgatgccg
    19741 gtttctgtta tccgccagaa accaaaaccg ttccgttaag cgaagtggtc gccaacaacg
    19801 cagcgtcaca gcctgtgtct gtttcgcagc aagagcagca caccgcgcaa ttgccctttt
    19861 ccgcgctctg ggcgttgttg atcggtattg gtatcgcctt tacgccatgc gtgctgccaa
    19921 tgtacccact gatttctggc atcgtgctgg gtggtaaaca gcggctctcc actgccagag
    19981 cattgttgct gacctttatt tatgtgcagg ggatggcact gacttacacg gcgctgggtc
    20041 tggtagttgc cgccgcaggg ttacagttcc aggcggcgct acagcaccca tacgtgctca
    20101 ttggcctcgc catcgtcttt accttgctgg cgatgtcaat gtttggcttg tttactctac
    20161 aactcccctc ttcgctgcaa acacgtctca cgttgatgag caatcgccaa cagggcggct
    20221 cacctggcgg tgtgtttgtt atgggggcga ttgccggact tatctgttca ccatgcacca
    20281 ccgcaccgct tagcgcgatt ctgctgtata tcgcccaaag cgggaacatg tggctgggcg
    20341 gcggcacgct ttatctctat gcgttgggca tgggcctgcc gctgatgcta attaccgtct
    20401 ttggtaaccg cttgctgccg aaaagcggcc cgtggatgga acaagtcaaa accgcgtttg
    20461 gttttgtgat cctcgcactg ccggtcttcc tgctggagcg agtgattggt gatgtatggg
    20521 gattacgctt gtggtcggcg ctgggtgtcg cattctttgg ctgggccttt atcaccagcc
    20581 tacaggctaa acgcggctgg atgcgcgtgg tgcaaataat cctgctggca gcggcattgg
    20641 ttagcgtgcg cccacttcag gattgggcat ttggtgcgac gcataccgcg caaactcaga
    20701 cgcatctcaa ctttacacaa atcaaaacgg tagatgagtt aaatcatgcg ctcgttgaag
    20761 ccaaaggcaa accggtgatg ttagatcttt atgccgactg gtgcgtcgcc tgtaaagagt
    20821 ttgagaaata caccttcagc gacccgcagg tgcaaaaagc gttagcagac acggtcttac
    20881 ttcaggccaa catcacggcc aacgacgcac aagatgtggc gctgttaaag catcttaatg
    20941 tccttggcct accgacaatt ctcttttttg acggacaagg ccaggagcat ccacaagcac
    21001 gcgtcacggg ctttatggat gctgaaacct tcagcgcaca tttgcgcgat cgccaaccgt
    21061 gaacgacact tgcagcggga aagacggagg aaaatagcgt gcaacgtgaa gatgtactgg
    21121 gagaagccct gaaattatta gaattacaag ggattgccaa caccacgctg gagatggttg
    21181 ctgaacgtgt ggattatcca ctggacgagc tacgccgctt ctggccagac aaagaggcaa
    21241 tcctctacga tgcgctgcgt tatcttagcc aacagataga tgtctggcgt cgtcagctga
    21301 tgctggacga aacgcaaacc gccgaacaaa agcagctggc acgttatcag gcgttatcgg
    21361 agtgcgttaa aaacaaccgc tatccgggct gtctatttat cgctgcctgt acgttttatc
    21421 ccgatcctgg ccaccctatt catcaactgg ccgatcagca aaaaagcgcg gcctacgatt
    21481 tcacccacga actgttaacc acactggaag ttgacgatcc ggcgatggta gcaaagcaga
    21541 tggaactggt gctggaaggc tgtttaagcc gaatgctggt gaatcgtagc caggcggatg
    21601 tcgacactgc acatcggctg gcggaagata tcctgcgctt cgcccgctgc cgccagggtg
    21661 gtgcattgac ctgacagaaa cacagaaaag aagcgatttg ccgcaatctt aagcagttga
    21721 atcgctttta ctgaaattag gttgacgaga tgtgcagatt acggtttaat gcgccccgtt
    21781 gcccggatag ctcagtcggt agagcagggg attgaaaatc cccgtgtcct tggttcgatt
    21841 ccgagtccgg gcaccaaatt catatcaacg gacctctacg gaggttcgtt tttcgtttca
    21901 gaacgccacg atttaagcgt tctgcctaca aatcaattct accgaactca accagattcc
    21961 ccccacatca accccattgt gtgggtataa ttgcgggcat accccagttc gacagaattt
    22021 gtaccctctt taacgcattc agaaggctga actatggcac tgactgacgc aaaaatccgg
    22081 gctgcaaagc ccactgacaa ggcttataaa ctcactgacg ggactggcat gttcctgctg
    22141 gtacatccta atggctcccg ttactggcgt ctccgttatc gtattctggg taaggagaag
    22201 actctggcac ttggtgtgta tccagaagtt tctctctccg aagctcgtac aaaacgggat
    22261 gaggcccgaa aactgatttc ggagggggtt gacccttgcg aacagaaaag agctaaaaaa
    22321 gtagtccctg atttacagct ctcttttgaa catattgcgc gacgctggca tgccagtaat
    22381 aaacaatggg cacaatcaca cagcgataaa gtactcaaaa gcctcgagac tcacgttttc
    22441 ccctttatcg gcaaccggga tatcacaaca ctcaataccc cggatctgct tatccctgtt
    22501 cgtgctgcag aagctaaaca aatttatgaa atcgccagtc gtctgcagca aagaatatct
    22561 gccgtaatgc gttatgccgt acagtctggc atcatcagat ataatcctgc tctggatatg
    22621 gctggcgcat tgactacggt aaaacgccag catcgccccg ctcttgatct ttctcgcctg
    22681 cctgaacttt tgtcgcgtat tggcagttac aaagggcaaa ctgtcaccca gcttgccgtt
    22741 atgctgaatt tactggtttt tattcgttcc agtgaactca gatacgcccg ctggtctgaa
    22801 attgatattg acaatgccat gtggactatt ccagccgaac gcgaacccct gccaggcgta
    22861 aaattctcac accggggctc caagatgcga acaccacatc ttgtgccact cagcaaacag
    22921 gctgtagcca tactgacaga actacagaca tgggctggtg aaaatggtct gatctttacg
    22981 ggagcacatg acccgcgtaa accaatcagt gaaaatgtaa tgaacgcatc
//
LOCUS       DAJTSL010000055        22931 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTSL010000055 DAJTSL010000000
VERSION     DAJTSL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.055"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..745)
                     /gene="fsr"
                     /locus_tag="OO433_001919"
     CDS             complement(<1..745)
                     /gene="fsr"
                     /locus_tag="OO433_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6050416.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSVGAVLLAAALVGTSSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLTAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISS"
     gene            963..2615
                     /gene="ushA"
                     /locus_tag="OO433_001920"
     CDS             963..2615
                     /gene="ushA"
                     /locus_tag="OO433_001920"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6050417.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVTKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(2652..3131)
                     /gene="ybaK"
                     /locus_tag="OO433_001921"
     CDS             complement(2652..3131)
                     /gene="ybaK"
                     /locus_tag="OO433_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6050418.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3335..4129)
                     /locus_tag="OO433_001922"
     CDS             complement(3335..4129)
                     /locus_tag="OO433_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6050419.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMTPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4267..4608
                     /locus_tag="OO433_001923"
     CDS             4267..4608
                     /locus_tag="OO433_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6050420.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(4823..7327)
                     /gene="copA"
                     /locus_tag="OO433_001924"
     CDS             complement(4823..7327)
                     /gene="copA"
                     /locus_tag="OO433_001924"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6050421.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPLW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNPALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7589..8521
                     /gene="glsA"
                     /locus_tag="OO433_001925"
     CDS             7589..8521
                     /gene="glsA"
                     /locus_tag="OO433_001925"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6050422.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAVVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGLN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8524..9816
                     /locus_tag="OO433_001926"
     CDS             8524..9816
                     /locus_tag="OO433_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6050423.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVAHAFGAYAVQFLHEGSQEEHLILLYALGSIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            9941..10348
                     /gene="cueR"
                     /locus_tag="OO433_001927"
     CDS             9941..10348
                     /gene="cueR"
                     /locus_tag="OO433_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6050424.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10349..10807)
                     /locus_tag="OO433_001928"
     CDS             complement(10349..10807)
                     /locus_tag="OO433_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6050425.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(10804..11721)
                     /locus_tag="OO433_001929"
     CDS             complement(10804..11721)
                     /locus_tag="OO433_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6050426.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            11867..12544
                     /gene="fetA"
                     /locus_tag="OO433_001930"
     CDS             11867..12544
                     /gene="fetA"
                     /locus_tag="OO433_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6050427.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12531..13310
                     /gene="fetB"
                     /locus_tag="OO433_001931"
     CDS             12531..13310
                     /gene="fetB"
                     /locus_tag="OO433_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6050428.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13373..14227)
                     /gene="cnoX"
                     /locus_tag="OO433_001932"
     CDS             complement(13373..14227)
                     /gene="cnoX"
                     /locus_tag="OO433_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6050429.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14288..15097)
                     /gene="ybbO"
                     /locus_tag="OO433_001933"
     CDS             complement(14288..15097)
                     /gene="ybbO"
                     /locus_tag="OO433_001933"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6050430.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15087..15710)
                     /gene="tesA"
                     /locus_tag="OO433_001934"
     CDS             complement(15087..15710)
                     /gene="tesA"
                     /locus_tag="OO433_001934"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR6050431.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            15681..16367
                     /locus_tag="OO433_001935"
     CDS             15681..16367
                     /locus_tag="OO433_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6050432.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16364..18775
                     /locus_tag="OO433_001936"
                     /pseudo
     CDS             16364..18775
                     /locus_tag="OO433_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            19204..22931
                     /locus_tag="OO433_001937"
                     /pseudo
     CDS             19204..22931
                     /locus_tag="OO433_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         5291 a         6004 c         6481 g         5155 t
ORIGIN      
        1 agctgctgat gctcgccata tagaaatatt tcgagaaaat gaggattaac agaatgctga
       61 ctgccagtac cactttgttg cgcggcagtg gattgattat cgtcgctttg ggttttcctt
      121 tattcattcg gtgctgtgcc gagtaccaac ggctaatttg cgccaacacc acgatcgcca
      181 gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc ataaggcgcg ataatcaccg
      241 ccgtcagcaa gggtcccagg gaactgccaa agttgccgcc gacctgaaag atagattgcg
      301 ccaggccatg ccgcccgccg gaagccatac gggctacgcg agaagattcc ggatgaaaga
      361 ccgatgaact ggtaccgacc agcgccgccg ccagcagaac tgcgccaaca ctgcccgcca
      421 gcgcaagcag caccagacca cttaaggtaa agcacatgcc aattggcaac gaccacggca
      481 tcggatattt atcggtccag tagccgacca ctggttgcag tagcgaagag gcgagctgga
      541 aggtgagggt tatcatgcca atctgcataa atgtcagaga aaattctgac tgaagcagag
      601 gataaatcgc cagaatcagc gattggatca tgtcgttcag cagatgtgag aggctgatag
      661 cacctaaaat accaaacgat gttcgggcct tggtcgttga cgcagccgcg cccgccacag
      721 gctgggtttg ttcactcatt gccataggaa agtcactttt tcagggttgc gatgtaaaga
      781 atgatcttat ttgtgattat tacccgacta acatacctgt aagcgtcgcc tgaaggaagt
      841 ctcaacgccg aatacagaat ttctaatctg gatgcagatt tatcttcacc ggacgcagac
      901 ttgtctatta tgttgcgtca tactattttt caacacgttg aaatcaggtc agggagagaa
      961 gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt aaccacattt acactggcga
     1021 gtgaaactgc tctggcgtat gagcaggata aaacctacaa aattacagtt ctgcatacca
     1081 atgatcatca tgggcatttt tggcgcaatg aatatggcga atatggtctg gcggcgcaaa
     1141 aaacgctggt ggatggtatc cgcaaagagg ttgcggctga aggcggtagc gtgctgctac
     1201 tttccggtgg cgacattaac actggcgtgc ccgagtctga cttacaggat gccgaacctg
     1261 attttcgcgg tatgaatctg gtgggctatg acgcgatggc gatcggtaat catgaatttg
     1321 ataatccgct caccgtatta cgccagcagg aaaagtgggc caagttcccg ttgctttccg
     1381 cgaatatcta ccagaaaagt accggcgagc gcctgtttaa accatgggcg ctgtttaagc
     1441 gtcaggatct gaaaattgcc gtcattgggc tgacaaccga tgacacagca aaaattggta
     1501 acccggaata cttcactgat gtcgaatttc gtaagcccgc cgatgaagcg aagctggtga
     1561 ttcaggagct gcaacagaca gaaaagccag acattattat cgcggcgacc catatggggc
     1621 attacgataa tggtgagcac ggctctaacg caccgggtga tgtggagatg gcacgcgcgc
     1681 tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc gcaagctccg gtctgcatgg
     1741 cggcagaaaa caaaaaacag gtcgattacg tgccgggtac gccatgcaaa ccagatcaac
     1801 aaaacggcat ctggattgtg caggcgcatg agtggggcaa atacgtggga cgggctgatt
     1861 ttgagtttcg taatggcgaa atgaaaatgg ttaactacca gctgattccg gtgaacctga
     1921 agaagaaagt gacctgggaa gacgggaaaa gcgagcgcgt gctttacact cctgaaatcg
     1981 ctgaaaacca gcaaatgatc tcgctgttat cgccgttcca gaacaaaggc aaagcgcagt
     2041 tggaagtgaa aataggcgaa accaatggtc gtctggaagg cgatcgtgac aaagtgcgtt
     2101 ttgtacagac caatatgggg cggttgattc tggcagccca aatggatcgc actggtgccg
     2161 actttgcggt gatgagtgga ggaggaattc gtgattctat cgaagcaggc gatatcagct
     2221 ataaaaacgt actgaaagtg cagccattcg gcaatgtggt ggtgtatgcc gacatgaccg
     2281 gtaaagaggt gattgattac ctgaccgccg tcgcacaaat gaagccagat tcaggtgcct
     2341 acccgcaatt tgccaacgtt agctttgtga cgaaagacgg caaactcaac gacctgaaaa
     2401 tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat ggcgacatta aacttcaatg
     2461 ccaccggcgg tgatggctat ccgcgccttg ataacaaacc gggctatgtg aataccggct
     2521 ttattgatgc cgaagtgctg aaagcgtata tccagaaaag ctcgccgctg gatgtgagtg
     2581 tttatgaacc gaaaggtgag gtgagctggc agtaatccga aagtgccgga tatttgcatc
     2641 cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt tggcatcgag gatcttcgcc
     2701 agatcgcctg ccgccagttc gatatccagt ccgcgcttgc cgccagaaac ataaatagtg
     2761 gcaaattctt gtgcgggggc gtcgataatc gtcggcagac gttttttctg ccccagtggg
     2821 ctaatccccc caaccaggta tcccgtcgaa cgctgcgcgg ccatcggatc ggccatctca
     2881 actttcttgg cacccagcgc ttttgctact tttttaagat ccagttgacc tgcgaccggc
     2941 gtaacggcca ctgccagatg tttcatatca ccgttcactg ccaccagtag cgttttgtag
     3001 acttgatccg gattcaaacc taattttttg acgacttcat cgccaaaatt ggtttcagcc
     3061 ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt ttttttcgag taatttaact
     3121 gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac gaagcaagca tacgcctgat
     3181 ggcggtccaa aaaaaagagt catcttgcct aagagtattg gcaggatggt gagattgagc
     3241 gacaatcgag ttacaccgtc gcttaaagtg acggcataat aataaaaaaa tgaaattcct
     3301 ctttgacggg ccaatagcga tattggccat ttttttagcg caacatttgc ggcaaattcc
     3361 cttctccata caggtgtagt gcaccgaccg cgaccacata tcgccccggc ggcatggcgc
     3421 gtaatttatc ccgccaggcg agatttcgct gatgcatcag cacatcgtac agcgactgac
     3481 tgaacgtatt gggcagcgtt atatcattat tttgcggagg tgcattcagc caccagctca
     3541 tcatttgttg cagcaaccgt gcgttggtat gccagtgggt cagcgtatcg tccagcagcg
     3601 ccagtccttt gtcagggagc tggagcaaca tggtaatctg gttttcagcc ccttccagtt
     3661 caatcacggg tttatgttgt tgcttcgccg cctgcaataa ctggtaatcg ataccgtatt
     3721 ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag aaccatcgcg atttgccaca
     3781 gcggttgagt agaaaagagc gacggagaaa tgcccatctc ctggctaatg tgctgcagat
     3841 tttggagttg ctcctcgtta atgcgctctt ccagcgcctc gcaagtaggc aaattagcaa
     3901 aaggcgtatc gctggtggaa acatccgcct cgacgatcag cgcatcggcg tttttgagct
     3961 ttttgagcaa acgggtgggc aggggagtca tatcgtggct acccatatga atactgccaa
     4021 tcagatgaaa atggcgattg ccgggaaggg tgatatcgat ggcaggccag gtgtaatgat
     4081 taccgcgcag cgcggcccaa agcgttttta cccggtacaa cagatccata cgacctccct
     4141 ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca aatggagttg gggacttgtt
     4201 taatgtgttt gtatgattca gtatgttctt gcatcgctat tcacagggaa gcaacagtta
     4261 aaaaccatga aacaggcaac aagaaaaccg acgacacccg gagatattct tctctatgaa
     4321 tatctggaac cgctcgattt gaaaatcaat gagttagcag agttgctgca tgttcatcgt
     4381 aatagcgtca gtacactgat caataataat cgtaaactca ccactgagat ggcattccgt
     4441 ctggcgaaag tttttgatac cacagtcgat ttttggctaa acctccaggc ggcagttgat
     4501 ctttgggaag ttgaaaacaa catgcgcacc caggaagaat tgggacggat tgaaacagtg
     4561 gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg tcgcgtaagg ccaaaacatc
     4621 gcttcatctc gtaggccgga taaggcgttc acgccgcatc cggcatccga gcaccatagc
     4681 ctgatgcgac gcttacccgt cttatcaggc ctacaaacct gtgcctgaac cgtaggccgg
     4741 ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg ccaaatgcgc cgccctaaag
     4801 cagcgcatcc gcaatgacgt acttattcct tcggtttaaa ccgcagcaac cggttggcgt
     4861 tactcactac ggtaatcgac gagagcgcca ttgccgctcc ggcaactacc gggttaagca
     4921 gtgttccagt aaacggccac aaaataccag cggcgaccgg aataccaatg ctgttgtaga
     4981 taaacgcacc gagcaggttc tgcttcatat tgcgcagcgt tgcgcgggaa atcgcgagcg
     5041 catctgcaac acccatcagg ctatggcgca tcagggtaat cgccgcggtt tcaatggcaa
     5101 catcactgcc gccacccatc gcaataccga catccgcctg agccagcgct ggcgcgtcgt
     5161 taatgccgtc gcccaccatt gccacctgac gtccttcact ttgcagacgt ttgatcgctt
     5221 cggctttacc gtctggcagc accccggcga ttacctcatc aatccctgct tctttggcga
     5281 tcgcattggc ggtggttggg ttatccccgg tcaacatcac cagacgatat cccgctttat
     5341 gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg atcgcgtaca gccagcaggg
     5401 ctactacttt cccgtcaatc gccagcagca caggcgttgc tccttgcgat gcctgagctg
     5461 aaatatcggc ttcaatcgct ttagtatcga cttgctggtc atttaacagc gccggattac
     5521 ccagcaataa cgcatgacct tcagcttcac cgcttacgcc cagcccgcgc aatgtgcgga
     5581 aaccgttgac ctgcggcagc tgcatatcgc ctgctttatc gaggatcgct cgtgccagcg
     5641 gatggctgga accttgctcc agcgccgccg ccagacgcaa tgcttgcgct tcatcaacat
     5701 cagcaaatgt ttttactgcg acaacctgcg gcttcccttc ggtcagcgtc ccggttttat
     5761 cgaacacgac ggtatcgagt gtactggcgc gttgcagcgc gtcggcgtcc cgcaccagca
     5821 cgccaaactc agccgcccgc ccgacgccgg aaataatcga cattggcgtc gccagcccca
     5881 gcgcacacgg acaggcaata atcagcaccg tggtggctat caccagggta tagacaatct
     5941 gcggtgccgg accaaagaaa taccagattg ctgcactgat cagcgcaatc accactacta
     6001 ccggcacaaa tacggctgag attttatccg ccagctgacc gatttctggc ttgctgctct
     6061 gggcctggcg caccatgcga ataatccttg acagcgtggt atggctgcca accgcactgg
     6121 cgcgaaatag cacgctaccg tcctgtacca ctgtcccggc atggacgctt tcgccttcgc
     6181 ctttttgctg cgggattggt tcgcccgtca gcatcgcttc atccagccat gcttcgccct
     6241 gggtaatctc gccatctacc ggcacgcgat cgccggtcgt caggcgcagt aacatccctg
     6301 gctgaacttc cgccagcggc acgctttttt caccctctgc ggtgaccact cgcgccgtcg
     6361 gtggggttaa atcgagtaat ttctccagcg ccttagaaga acgctgacgc gcgcgcgctt
     6421 ccagcatatg gccgagatta atcagaccga taatcatcgc actggcttca tagtaaagat
     6481 gccgcgcttc catcgggaac cacagcggcc acaggttgac gctcatcgaa tagagccacg
     6541 ccacacccgt acccagcgcc accagcgtat ccatcgtcgc cgcaccgttc agcaggcttt
     6601 tccacgcgct gcggtaaaaa tgcccgccgg cgaaaaccat taccgccagg gttatcaggc
     6661 cgataactaa ccacaggctg cggttgtcag cggtgaccat catgttatcg ccgatcatcc
     6721 cccagaccat taccggaata cccaccgcca gtgcgacaat cgcctgccag cggaagcgct
     6781 tcatcgtagc gacggcggtt tcctgctggc gctcgcggcg tttcgcgtca tcttcaatcg
     6841 cttccgcgcc gtagcccgct ttttccaccg cctgcactaa atcttgtggg gaggcactgc
     6901 ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc ctgagtgacg cccggtacgc
     6961 tttgcagcgc attttgtacg cgggtgacac agctggcgca gctcatgccg ctcagcagca
     7021 actgctggct gtcatcgtca tcggcggtcg ctgccggaag cgcctcagaa accgctgtca
     7081 gtgcttccga cgggattgat gactccgcca gcggtttagc ctttgggtgg cttacagatg
     7141 cgtcataacc cgcttgtttg atggtttcga tcagctgttc tgcactggca gtcccggtaa
     7201 cgtgcgcttc agtgatagac acatccgctt gttccacatc cgggcgctgt tcgagacttt
     7261 ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc cagggtcagg tcgatagttt
     7321 gtgacataaa acactccttt aagacagttt tgactggctg tgataaaggt taaaccttcc
     7381 agcaagggga aggtcaagaa attaataaac caggcgggta aaagtccgta aagattaaaa
     7441 aatcggctcg atttgcatca ggattagaca tttatttctt tgttttcctg tagttaagtt
     7501 gctggtgcta agttaaatca ggatgcctga aaatcggcac cggggtgagg aattacctcc
     7561 cgcatctata aaaaggagtt aacaaaagat gttagatgca aacaaattac agcaggcagt
     7621 ggatcaggct tacacccaat ttcactcact taacggcgga caaaatgccg attacattcc
     7681 ctttctggcg aatgtaccag gtcaactggc ggtagtggct atcgtgacct gcgatggcaa
     7741 cgtctatagt gcgggtgaca gtgattaccg ctttgcactg gaatccatct cgaaagtctg
     7801 tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg gtacaggaca aaattggcgc
     7861 tgacccgacc ggattgccct ttaactcagt tatcgcctta gagttgcatg gcggcaaacc
     7921 gctttcgcca ctggtaaatg ctggcgctat tgccaccacc agcctgatta acgctgaaaa
     7981 tattgaacaa cgctggcagc gaattttaca tatccaacag caactggctg gtgagcaggt
     8041 agcgctctct gacgaagtca accagtcgga acaaacaacc aacttccata accgggccat
     8101 agcctggctg ctgtactccg ccggatatct ctattgtgat gcaatggaag cctgtgacgt
     8161 gtacacccgt cagtgctcca cgctcatcaa tactgttgaa ctggcaacgc ttggcgcgac
     8221 gctggcggcg ggtggtctga atccgctgac gcataaacgc gttcttcagg ccgacaacgt
     8281 gccgtacatt ctggccgaaa tgatgatgga agggctgtat ggtcgctccg gtgactgggc
     8341 gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc ggtattctgg cggtcgtccc
     8401 tggcgtgatg ggaattgccg cgttctcacc accgctggac gaagaaggta acagtgttcg
     8461 cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc tataacgtgt ttaagggctg
     8521 atcatgatga acacggaagg taataacggt aacaaacccc tcggtctatg gaatgtcgtt
     8581 tccatcggta ttggggcaat ggtgggggcg gggatcttcg cgctgctggg gcaggctgcg
     8641 ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg gcggtattgt ggcgatgttt
     8701 tccggttatg cctatgcgcg tctgggggcg agctatccca gcaatggcgg cattatcgac
     8761 ttctttcgtc gcggattagg caacggcgtc ttttcgctgg cgctctcgtt actgtacctg
     8821 ttgacgctgg cggtgagcat cgccatggtc gcccatgctt ttggcgctta tgccgtgcag
     8881 tttttgcatg aaggcagcca ggaggagcac cttattttgc tctacgcgtt ggggagcatt
     8941 gcggtgatga cgcttttcaa ctccttaagc aaccatgcgg tagggcggct ggaagtgatc
     9001 ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta ttgccggtgt ctggtcgctg
     9061 caaccagcgc atatttccgt ctctgcgccc cccagctccg gtgcgttctt ctcctgtatt
     9121 gggataactt tccttgccta tgcgggcttt ggcatgatgg cgaacgcggc ggataaagtg
     9181 aaagatccgc aggtcattat gccacgggcg tttctggtgg cgattggcgt taccacgttg
     9241 ctttatatct cgctggcact ggttttgctt agcgatgtat cggcattaga gttagaaaaa
     9301 tatgccgata ccgccgtagc gcaggctgct tctccgctgc tcggacatgt gggttatgtg
     9361 atcgtcgtca tcggcgcttt actggcgacg gcttcagcca ttaacgcgaa cctgttcgcc
     9421 gtgtttaaca tcatggacaa catgggcagc gaacgcgaac tgccgaagct aatgaataaa
     9481 tccctgtggc ggcagagtac ctggggcaac atcattgtcg tggtgttgat tatgctgatg
     9541 acggcggcac tgaatttagg ctcactcgcc agcgttgcca gcgccacctt tttgatttgc
     9601 tacctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg atattcacgc ctcgttgccg
     9661 attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg ttggctttat ctacagtctg
     9721 tggtcccagg gtagccgtgc gttgatatgg attattggct cactcttact cagccttatt
     9781 gtggcaatgg tcatgaagcg caataaaacc gtataacatc tctctgtgcg cagtacttcc
     9841 tgtattattg tggtggcggt cgatattcgc actggcaaaa aaacgtgctt gaatatctgt
     9901 tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg atgaacatca gcgatgtagc
     9961 aaaaattacc ggcctgacta gcaaagccat tcgcttctat gaagagaagg ggctggtgac
    10021 gccgccgatg cgcagcgaaa acggttatcg cacctacacg cagcagcatc tcaacgaact
    10081 gaccttactg cgccaggcac ggcaggtggg ctttaacctg gaagagagcg gcgagctggt
    10141 gaatctgttt aacgacccgc agcggcacag cgccgacgtc aaacggcgca cgctggagaa
    10201 ggtggcggag atcgaacgac acattgagga gctgcaatcc atgcgcgacc agctgctggc
    10261 actggcgaat gcctgccctg gcgatgacag cgccgactgc ccgattatcg aaaatctctc
    10321 cggctgctgt catcatcggg cagggtgatt aagacgagac ggctcggata tgtagggtta
    10381 tcccttctat cgcaatgact tcaacatgcg tacctgcacc gagatcctcg ctggcgctga
    10441 caggccatga actgtcaccg acgcgcatat gaccgcgccc gttgaccagc ggagattcca
    10501 gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt taaatgacta tcgctgtgct
    10561 tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg agcagcgtca
    10621 ggatggcaaa cattacccct tgccactccc aacccagcgg caccagccag accaccaggc
    10681 cggtaatcac cgctgccacg ccgctccaca acaaataacc atttccgccc agcatctcgg
    10741 ctgccagcag caaaccgccg agactgagcc agaaaatatg tggatgaacg accattaact
    10801 ccatcatggc tgagtccgtt tgttggcgct gtctttcacc agttcggcaa tcccggcaat
    10861 cgaccccatc aggctgctgg cctctaatgg catcatcact actttgctgt tactggagga
    10921 accgatctgc tgtaacgctt cggtgtattt ctgcgctacg aagtagttca ccgcctgaat
    10981 atcaccggag gcgatagctt cagacaccat tttggtggcg cgggcttctg cttcggcgga
    11041 acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg ctttcaggat
    11101 ttgcgactgt ttttcacctt cggctttgag gatttccgcc tgacggatcc cttccgcttc
    11161 aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg aagagataag
    11221 ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg actttaatcc cccacgggtt
    11281 ggtggcttca tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct gagagagcat
    11341 ttcgtcaagt tccattgaac ccaacacggt acggatgtta gtcatggtca ggttgatgat
    11401 cgccagctcc agattgctga cttcataggc agcgcgtggc gcgtcaatca cctgaataaa
    11461 acagactgcg tcgatggtaa cgttggcgtt atctttcgag ataacttcct gggaagggat
    11521 atcgagcact tgctccatca tattgatctt gcgaccaatg cgatccataa acggcaccac
    11581 cagactgagc cccggctgta acgttttggt atagcgacca aaacgttcta ccgtccattg
    11641 atagccctgc ggcacgattt tgacgcccgc gccgacaatg accagcgcga caaaaatgag
    11701 aatcgggata aagataagca tcggaaaaac ctcctgttgt accgtccata atcagcaaaa
    11761 ttgctgcttg attaaacaaa ttatacctga ttactgaaag agagttcccc cttattcctg
    11821 cgaaggataa actgttttta gtaaaaatca gaaaaaggga acagcgatgc aggaaaatag
    11881 tcctttgctt cagctacaaa acgtaggata tctggcgggt gatgcgaaga ttcttaataa
    11941 catcaatttt tcgctgcgtg ctggcgaatt taagttaatt accggtcctt ctggttgtgg
    12001 caaaagtacg ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg gaacgttact
    12061 gtttgaaggt gaggatgtca gcacactaaa gccagaaatc taccgccaac aagtctctta
    12121 ctgcgcccag acaccgacgc tgtttggcga tacggtatac gataatctga tctttccttg
    12181 gcagatccgt aaccggcagc ctgacccagc catttttctc gattttctcg aacgcttcgc
    12241 cttgccggac agcattttga cgaaaaatat cgccgagcta tctggtggtg aaaaacaacg
    12301 catctcactg attcgtaacc tgcaatttat gccgaaggtt ttattgctgg atgaaatcac
    12361 cagtgcgctg gatgaaagta ataaacataa cgttaatgag atgatccatc gttatgtgcg
    12421 cgagcaaaat attgccgtgc tgtgggtgac acacgataaa gacgaaatta atcatgcgga
    12481 taaagtgatt acactgcaac cgcatgccgg agaaatgcag gaagcacgct atgaactcgc
    12541 ataatattac taacgaatca ttggcactgg cattaatgct ggtggtggtg gcaatcttaa
    12601 ttagccataa agaaaaactg gcgctggaga aagatatttt ctggagcgtc gggcgcgcga
    12661 taattcagct gattattgtc ggctatgtgc tgaagtatat tttcagcgtg gatgatgcca
    12721 gcctgacatt attgatggtg ttatttatct gctttaatgc ggcgtggaac gcgcaaaaac
    12781 gcagtaaata tattgctaaa gcttttatct catcgtttat tgccattacg gttggggcgg
    12841 gaattaccct ggcggtgctg attctctcag ggtcgattga atttatcccg atgcaggtga
    12901 tccctatcgc cgggatgatt gccggtaacg ccatggtagc ggtggggttg tgttacaaca
    12961 atttagggca acgggtcatt agcgaacagc aacagattca ggagaaactg agtcttggcg
    13021 cgacgccgaa gcaggcttca gcgatattga ttcgcgacag tattcgcgcg gctttaattc
    13081 cgacggtcga ttcagcaaaa acggttggct tagtgagttt accaggaatg atgtccgggc
    13141 tgatatttgc cgggattgat ccggtgaagg cgattaaata tcagattatg gtgaccttta
    13201 tgctgctctc aaccgccagc ttgtcgacca ttattgcctg ctatttaacc tatcgtaagt
    13261 tttataattc gcgccaccag ttggtggtga cgcaattgaa gaagaaatga tgcgatgccg
    13321 gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg aacaacggaa tatcaataca
    13381 acaatgcata cagctggcgg cgatacttcg acgccagtgc atcacccgta cccagcgcag
    13441 cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc ggcggtgaga tctttacgca
    13501 gatgcccgaa cagcaactcc agcgcctctt cattgcgccc aacctgatgc agttgcagcg
    13561 ccagttgcgt cgccagtgcg gcatcttctg gattctccgc cacctgctgt tgcaactgtt
    13621 gaatttccgg cgtatcagcc gcctgcttca gcagttcgat ttgcgccacc agcccctggt
    13681 agcgggtgtc ctgatcctgc aacggaatgg ttttcagcac cgcttccgca tcttcagaac
    13741 ggttcagcgc aatcagcgtt tctgccagca gcaggccgat ctccccgttc tgattcgaca
    13801 actgccaggc gtctttcagc aatggcaggg catcggtgta attgccttcc tgcatcagtt
    13861 gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag cactttatcc agcagggcgc
    13921 ggatcgcctc ttccggttgc ggcccctgga agccatctac cggttgcccg ttctggaaca
    13981 gatacacggt cggaatcgcg cgcagaccaa actgggcggc aatcatctgc tccgcgtcgc
    14041 agtccagctt cgccagaata aattgcccgt tgtactgcgc cgcgaggctt tccaggatcg
    14101 gggttaactg caaacagtgc tggctacgtt cagaccaaaa atagaacagc accggagtgg
    14161 tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca atattttcta
    14221 cggacatgga gtcgctctct gttgtcgata ttttctttga catgggggct taagcgcgcg
    14281 cttcaactca cccctgcaat attttgtcca tcacgcgccc aggcagcagg cgcttgagca
    14341 ccattactgc ccaggtcacc agcgttaccg gatagcgcat cttcggcttc tcgctaataa
    14401 aagcatggcg cactttgtcg accaccgctt ccggtcccaa cgtaaagcgg gcggcgatgc
    14461 cgggattttc gactggttta tcactttgcg tctggttgac gttgtcggtg aagcgagtac
    14521 gaatgggacc gggttcgatc aggctgactt taattccgct gtggcgtagc tccatgcgca
    14581 gcgcgtctga ccacgcctcc agcgcatatt tactggccgc gtaagcgcca cgacccggcg
    14641 tggagattaa tcccatcacc gatgatgtca tcacaatacg cccttcaccg tgcggtaaca
    14701 tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa aaagttggcg gaaaactgct
    14761 gttccatctg cgcacggctg atggtggaaa gggggccata catgccgaat ccggcattgt
    14821 taaagatccc atacagacaa ttatcggtca gggcgatcac ctcgtctgct gcgcgatcaa
    14881 cactttctgg tgaatccaga tcgatcaaca cgccggtaaa tcccatgttg ttcatacgct
    14941 caacatcatc cggttttcgg caacctgcca gcacatgaaa accctggcgt tttaattcga
    15001 gcgcgctttc caggccaatt ccactggaac atccggtaat taagaccgat ttttgcataa
    15061 ctttacctgt caggatctcc gttgctttat gagtcatgat ttactaaagg ctgcaactgc
    15121 ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat accgtcatcc
    15181 tgcatccatt gtggcttgag gtagacctct tccataaaaa agggcagcag cggaacatca
    15241 aactctttgg cgagtttggg gtaaatggcg ctaaaggctt cattataacg gcgaccatag
    15301 tttgcaggca gacgtatttg cattaacaat ggttcagcgt tggcggcttt gacatcctgc
    15361 aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa aaccacgcaa accgtcattg
    15421 ccgcccagtt caaccagcac ccagcgcggc tgatgctgtt tcagcagagc cggaaggcgc
    15481 gccagtcctt gttgcgaggt gtcgccgctg atgctggcat taactaccga cgttttactc
    15541 tgccacttat cattcaacaa ggcaggccag gccgcgctgg cagacattcg atacccggcg
    15601 ctcaggctat cacccagaat caataacgtg tccgctgcgg cggcacggaa ggttaacagg
    15661 accaggaaca ggaagggcaa atgccagcgg aaaacattgt tgaagttcat catcttaaga
    15721 agtccgtcgg tcagggggag catgaactct ccatcctcac cggagttgag ctggttgtca
    15781 aacgtggcga gaccatcgca ctggtgggcg agtcgggatc gggtaagtca accttgctgg
    15841 cgatcctcgc cgggcttgat gacggcagca gtggcgaagt gagtctggtg ggacaaccgc
    15901 tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc gaagcacgtc ggctttgttt
    15961 ttcagtcatt tatgttaatt cctaccctta acgcgctgga aaacgtcgag cttccggctc
    16021 tgctgcgcgg tgagagtagc gcggaaagtc gtaacggggc gaaagcgttg ctcgaacagt
    16081 tagggctggg taaacgtctg gatcatcttc cggcacagct ttccggcggt gaacagcaac
    16141 gagtggcgct ggcacgagcc tttaatggtc gacctgatgt gctgtttgcc gacgaaccca
    16201 ccggcaacct tgaccgccag acgggcgata aaattgccga cctgctgttt tccctcaacc
    16261 gtgaacatgg caccacgttg attatggtga cccacgacct gcaactggcg gcacgctgcg
    16321 accgctgctt acggctggtg aacgggcagt tgcaggagga agcatgattg cacgttggtt
    16381 ctggcgcgaa tggcgttcgc cgtcgctatt aattgtctgg ctggcgctaa gcctggcggt
    16441 ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg gagaagggct taagccagca
    16501 aagccgtgag tttatggcgg gcgatcgggc gttgcgcagt tcacgcgaag tgccgcaagc
    16561 gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc aagcagctga ctttcgccac
    16621 aatgaccttt gcaggcgaca caccgcagct ggcgaacgtc aaagcggtgg atgatatcta
    16681 cccgatgtat ggcgatctgc aaactaatcc ccctggcctg aaaccgcagg cgggcagcgt
    16741 attgctggcc ccacgcctga tggcactgct taacctgaaa acgggcgaca ccattgacgt
    16801 gggcgatgcc accttgcgga ttgccggaga agtgattcag gaaccggatt ccggttttaa
    16861 ccccttccag atggctccgc gtctgatgat gaatctggcg gatgtcgata aaaccggagc
    16921 cgtgcagccg gggagtcggg tcacctggcg ttataaattc ggcggcaacg agaaccagct
    16981 cgacggctat gagaaatggt tgttacctca gcttaaaccc gaacaacgct ggtacggtct
    17041 ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc tcgcaacagt tcctgctgct
    17101 ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg gcggtagcga tgaatcatta
    17161 ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg ctgggggcag ggcgaacgca
    17221 actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg acgctttcag ccgttaccgg
    17281 tggggccata ggcctgttgt tcgaaaacgt gttgatggtg ctgctcaagc ccgttctgcc
    17341 tgctgcacta ccgccagcca gcctctggcc gtggctgtgg gcgcttggca ccatgacggt
    17401 catctcgctg ctggtggggc tacgaccata ccgcttgttg ctggcaacgc agcctttacg
    17461 cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg aagttttatc tgccgattgt
    17521 cagtatggtg gttgtgctgc tgctcgccgg attaatgggt ggcagcatgc tgctttgggc
    17581 ggtgctggcg ggcgcagtag tactggcttt gctgtgcggt gtgctgggct ggatgcagct
    17641 gaatgtactt tgccgcatga cgctgaaatc gctacctctg cgcctggcgg ttagccgcct
    17701 gttacgtcag ccgtggtcaa cgttaagcca gctttcggca ttttcgctct cctttatgct
    17761 gctggcgctg ttgctggtat tgcgttgcga tctgctcgac cgctggcagc agctaccgcc
    17821 agaaagcccg aactactttt taattaacat cgccacagaa caggtagcac cgctaaaagc
    17881 gttcctcgcg gaacatcata tagtcccgga atcattttat ccggtagtgc gggcgcggct
    17941 gacggcgatt aacgataagc cgacagaagg taatgaagat gaggcgctta atcgcgaact
    18001 caatcttacc tggcaaaata cgcggcccga tcataatccg attgtcgccg gtaactggcc
    18061 gccaaaagcc gatgaagtgt cgatggaaga ggggctggcg aaacgcttaa acgttgccct
    18121 cggtggtacc gtgactttta tgggcgatac ccaggagttc cgcgctaaag tgaccagcct
    18181 gcgcaaagtg gactgggaaa gtctgcggcc taatttctat tttattttcc cggaaggggc
    18241 attagacggg caaccgcaga gctggcttac cagtttccgc tgggagaatg gcaacggcat
    18301 gttgacgcaa ctcaaccgcc agttcccgac cattagcctg ttagatattg gcgcgatttt
    18361 aaaacaggtc ggttaggtgc tggagcaggt aagtcgggcg ctggaggtga tggtggtgct
    18421 ggtcaccgcc tgcggtatgt tgctgttgct ggcacaggtg caggtgggaa tgcgccagcg
    18481 tcatcaggag ctggtggtat ggcgcacact cggtgcgggg aaaaaactgc tgcgcacgac
    18541 attgtggtgt gagttcgcca tgcttgggtt tgtttccggc ctggtggccg caattggtgc
    18601 ggaaacggca ctggcagtgt tgcaggcgaa agtgtttgat ttcccgtggg agccagactg
    18661 gcgattgtgg attgttctgc cgtgcagcgg tgcgctgctg ctgtcgcttt tcggcggctg
    18721 gctgggtgcg cgactggtta agggtaaggc gctgttcagg cagtttgcgg ggtgatgaga
    18781 gtaaataagg ggcgttctgg ttgaatggaa cgccttgttt aaattaattc tacaatatgt
    18841 ttcattgatg atgttattga attggtgatt tcctatcttt ctattgctga tattaatctg
    18901 aattggtgga gtttaataat tttaaatata gccctacaaa aataactcag actaataaaa
    18961 tcataaatca tatgcgttga atggatatta tccatatagt gaatttgttg atgatgaatt
    19021 catctgtgct aaaaatttta gtttaataaa atattgaaag tgacctgtaa taacagttat
    19081 tgttgattga gaacaaataa gtttatgtga aaaatatata aatacattag ctggtcatgt
    19141 gtgtcatttt atttttttgt tgctaacaca gggatatgaa caataactaa aagggcactt
    19201 tatatgagcg gaaaaccagc ggcgcgtcag ggagatatga ctcagtatgg cggtcccatt
    19261 gtccagggtt cggcaggtgt aagaattggc gcgccaactg gcgtggcgtg ctcggtgtgt
    19321 ccgggcggga tgacctcggg caacccggtg aacccgctgc tcggtgcaaa agtacagccc
    19381 ggcgagacgg accttgcgct gcccgacccg ctgccgttca ttctctcccg cacctacagc
    19441 agctaccgga cgaagacgcc tgcgccagtg ggaacttttt ggccccggct ggaaagcgcc
    19501 ttctgatatc cgcttacaga tacgcgatga tgcactggta ctcaacgaca acggcgggcg
    19561 gagcattcac tttgagccgc tgctgccggg gaaggctgtg tacagccgca gtgagtcaat
    19621 gtggctggtg cgtggcggta aggcagcgca gccggacggc cacacgctgg cacgactgtg
    19681 ggcgtcgctg ccgccggata tccggttaag cccgcatctt tacctggcga ccaacagcgc
    19741 acaggggccg tggtggatac tggggtgatc tgagcgggtg ccgggtgctg aggacgtact
    19801 gccagcgccg ctgccgccgt accgggtgct taccgggatg gcggaccgct tcgggcggac
    19861 gctgacgtac cggcgtgagg ccgcaggcga cctggccggg gaaatcaccg gcgtgacgga
    19921 cggtgccggg cgggagttcc gtctggtgct gaccacacag gcgcagcagg cggaagaggc
    19981 ccggcagcag gcggtcgtcg ccggagcaaa acgaccggac attcctgatt cactgccgga
    20041 gagcacagag tacggccccg acaggggtat ccgcctttcg gcggtgtggc tgatgcacga
    20101 cccggcatac ccggagagcc tgcccggtgc gccactggcg cggtacacgt atacggaagc
    20161 cggtgaactg ctggcggtat atgaccgcag caatacgcag gtgcgcgctt tcacgtatga
    20221 cgcgcagcac ccgggccgga tggtggcgca ccgttacgcg ggaaggccgg agatgcgcta
    20281 ccgctacgac gatacggggc gggtggtgga gcaactgaac ccggcagggt taagctaccg
    20341 ctatctttat gagcaggacc gcatcaccgt caccgacagc ctgaaccggc gtgaggtgct
    20401 gcatacagaa ggcggggccg ggctgaaacg ggtggtgaaa aaagaactgg cggacggcag
    20461 cgtcacgcgc agcgggtatg acgcggcagg aaggctcacg gcgcagacgg acgcggcggg
    20521 acggcggaca gagtacggtc tgaatgtggt gtccggcgat atcacggaca tcaccacacc
    20581 ggacgggcgg gagacgaaat tttactataa cgacgggaac cagctgacgg cggtggtgta
    20641 cccggacggg ctggagagca gccgggaata tgatgaaccg ggcaggctgg tatcggagac
    20701 atcgcgcagc ggggagacag tacgctaccg ctacgatgac gcgcacagtg agttaccggc
    20761 gacgacaacg gatgcgacgg gcagcacccg gcagatgacg gcggttcacc gcgaggaagg
    20821 tatcagcctt taccgccact atgacaaccg tggccggtta acctcggtga aagacgcaca
    20881 gggccgtgaa acgcagtatg agtacaacgc cgcaggcgac ctgactgccg ttatcacccc
    20941 ggacggcaac cggagcgaga cacagtacga tgcgtgggga aaggcggtca gcaccacgca
    21001 gggcgggctg acgcgcagta tggagtacga tgctgccgga cgtgtcatca gtctgactaa
    21061 cgagaacggc agtcacagcg acttcagcta cgatgcgctg gaccggctgg tacagcaggg
    21121 cggctttgac gggcggacgc aacgttatca ttatgacctg accggaaaac tcacgcagag
    21181 tgaggatgag gggcttgtca ccctctggca ctacgatgcg tcggaccgca tcacgcaccg
    21241 gacggtgaac ggcgacccgg cagagcagtg gcagtatgac ggccacggct ggctgagaga
    21301 aatcagccac ctgagcgaag gccaccgtgt cgctgtccac tacggctacg acgataaggg
    21361 ccgcctgacg ggcgaacggc agacggtgga gaacccggag acgggggaac tgctgtggca
    21421 gcatgagacg aaacacgcct acaacgaaca ggggctggca aaccgcgtca cgccggacag
    21481 cctgccgccg gtggagtggc tgacctacgg cagcggttac ctggcgggca tgaagctggg
    21541 cgggacgccg ctggtcgagt atacgcggga caggctgcac cgtgagacgg tgcgcagctt
    21601 cggcagcatg gcaggcagta atgccgcata tgaactgacc agcacataca cccccgcagg
    21661 ccagttacag agccagcacc tgaacagcct ggtgtatgac cgtgactacg ggtggaatga
    21721 caacggcgac ctggtgcgca tcagcggtcc gcgacagacg cgggaatacg gctacagcgc
    21781 cacgggcagg ctggagagcg tgcgcaccct cgcaccggac ctggacatcc gcatcccgta
    21841 tgcgaccgac ccggcgggaa accggctgcc ggacccggag ctacacccgg acagcacgct
    21901 cacggcgtgg ccggataacc gcatcgcaga ggatgcgcac tatgtttacc actatgatga
    21961 atacggcaga ctgacggaga agacggaccg catcccggcg ggtgtgatac ggacggacga
    22021 cgagcggacc caccactacc actacgacag ccagcaccgc ctggtgttcc acacgcggat
    22081 acagcatggc gagccactgg tcgagagccg ctacctctac gacccgctga gccgccggac
    22141 ggggaaacgg gtgtggcgtc gcgagcgtga cctgacgggg tggatgtcgc tgtcgcgtaa
    22201 accggaggtg acgtggtacg gatgggacgg cgaccggctg acgacggtac agaccgacac
    22261 cacgcgtatc cagacggtat accagccggg gagcttcgcg ccgctcatcc gcatcgaaac
    22321 agacaacggt gaacgggaga aagcgcagtg ccgcagcctg gcggagaagt tacagcagga
    22381 agggagcgaa gacggtcacg gtgtggtgtt cccggctgaa ctggtggggc tgctggacag
    22441 gctggagggg gaaatccggg caaactgcgt gagcagtgaa agccgccagt ggcttgcgca
    22501 gtgcgggctg acggtggagc ggctggcggc acagatagag ccggtttatt tgcctgagcg
    22561 aaaaatacat ctttatcact gtgaccaccg gggcctgccg ctggcgctta tcagtgaaga
    22621 cggcaatacg gcgtggagcg cggaatatga tgaatggggc aaccagctta atgaggagaa
    22681 cccgcatcac ctgcaccagc cgtaccggct gccggggcag cagtatgata aggagtcggg
    22741 gctgtactac aaccggaacc ggtactacga tccgttgcag gggcggtata tcactcagga
    22801 cccgataggg ctggaggggg gatggagtct gtatgcgtat ccgctgaatc cggtgaatgg
    22861 tattgatcca ttagggttaa gtcccgcaga tgtagcgcta ataagaagaa aagatcaact
    22921 aaaccatcaa a
//
LOCUS       DAJTSL010000056        22620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTSL010000056 DAJTSL010000000
VERSION     DAJTSL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.056"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(50..1699)
                     /gene="actP"
                     /locus_tag="OO433_001938"
     CDS             complement(50..1699)
                     /gene="actP"
                     /locus_tag="OO433_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6050433.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGMLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGTMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1696..2010)
                     /locus_tag="OO433_001939"
     CDS             complement(1696..2010)
                     /locus_tag="OO433_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6050434.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2142..4101)
                     /gene="acs"
                     /locus_tag="OO433_001940"
                     /pseudo
     CDS             complement(2142..4101)
                     /gene="acs"
                     /locus_tag="OO433_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4494..5930
                     /gene="nrfA"
                     /locus_tag="OO433_001941"
     CDS             4494..5930
                     /gene="nrfA"
                     /locus_tag="OO433_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6050435.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5975..6541
                     /gene="nrfB"
                     /locus_tag="OO433_001942"
     CDS             5975..6541
                     /gene="nrfB"
                     /locus_tag="OO433_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6050436.1"
                     /translation="MSVLRSLLTARVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6538..7209
                     /gene="nrfC"
                     /locus_tag="OO433_001943"
     CDS             6538..7209
                     /gene="nrfC"
                     /locus_tag="OO433_001943"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6050437.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7206..8162
                     /gene="nrfD"
                     /locus_tag="OO433_001944"
     CDS             7206..8162
                     /gene="nrfD"
                     /locus_tag="OO433_001944"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6050438.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLAQQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKSWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8182..9900
                     /locus_tag="OO433_001945"
     CDS             8182..9900
                     /locus_tag="OO433_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6050439.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9893..10276
                     /gene="nrfF"
                     /locus_tag="OO433_001946"
     CDS             9893..10276
                     /gene="nrfF"
                     /locus_tag="OO433_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6050440.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10273..10869
                     /gene="nrfG"
                     /locus_tag="OO433_001947"
     CDS             10273..10869
                     /gene="nrfG"
                     /locus_tag="OO433_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6050441.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYVALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11210..12523
                     /gene="gltP"
                     /locus_tag="OO433_001948"
     CDS             11210..12523
                     /gene="gltP"
                     /locus_tag="OO433_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6050442.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12714..13402)
                     /locus_tag="OO433_001949"
                     /pseudo
     CDS             complement(12714..13402)
                     /locus_tag="OO433_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(13496..15643)
                     /gene="fdhF"
                     /locus_tag="OO433_001950"
     CDS             complement(13496..15643)
                     /gene="fdhF"
                     /locus_tag="OO433_001950"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15224..15226),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6050443.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIMANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGEQGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15841..17307)
                     /gene="mdtP"
                     /locus_tag="OO433_001951"
     CDS             complement(15841..17307)
                     /gene="mdtP"
                     /locus_tag="OO433_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6050444.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPSLGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGIAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIHSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17304..19355)
                     /gene="mdtO"
                     /locus_tag="OO433_001952"
     CDS             complement(17304..19355)
                     /gene="mdtO"
                     /locus_tag="OO433_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6050445.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSCSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19355..20386)
                     /gene="mdtN"
                     /locus_tag="OO433_001953"
     CDS             complement(19355..20386)
                     /gene="mdtN"
                     /locus_tag="OO433_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6050446.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDVLVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPNDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20405..20680)
                     /locus_tag="OO433_001954"
     CDS             complement(20405..20680)
                     /locus_tag="OO433_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050447.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFAVYAMLFWLAFF"
     gene            complement(20889..>22620)
                     /gene="yjcS"
                     /locus_tag="OO433_001955"
     CDS             complement(20889..>22620)
                     /gene="yjcS"
                     /locus_tag="OO433_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR6050448.1"
                     /translation="RGEDYKFDINAPAPETVNPSLWRQSQLNGISGLFKVTDRMYQVR
                     GQDISNITFIEGDTGIIVIDPLVTPPSAKAALDLYFQNRPQKPIVAVIYTHSHTDHYG
                     GVKGIISEADVKSGKVQVIAPAGFMDEAISENVLAGNIMSHRALYSYGLLLAHNPQGN
                     IGNGLGVTLASGYPSIIAPNKTITKTGEKMIIDGLEFDFLMTPGSEAPAEMHFYIPAL
                     KALCTAENATHTLHNFYTLRGAKTRDTSKWTEYLNETLDMWGNDAEVLFMPHTWPVRG
                     NKHINDYIGKYRDTIKYIHDQTLHLANQGYTMNEIGDMIKLPPALANNWASRGYYGSV
                     SHNARAVYNFYLGYYDGNPANLHPYGQVEMGKRYVQALGGSARVISLAQEANKQGDYR
                     WSAELLKQVIAANPGDQVAKNLQANNFEQLGYQAESATWRGFYLTGAKELREGVHKFS
                     HGTTGSPDTIRGMSVEMLFDFMSVRLDSAKAAGKNISLNFNMSNGDNLNLTLNDSVLN
                     YRKTLQPQADASFYISREDLHAVLTGQAKMADLVKAKKAKIIGNGAKLEEIIACLDNF
                     DLWVNIVTPN"
BASE COUNT         5075 a         5961 c         6195 g         5389 t
ORIGIN      
        1 ctggagagga agagtattcc ccggtcaggc gaccggggaa ggggagagat taatgtgcgc
       61 ggccttgctc aacgccaaag ccggtctggg agcggataaa ctgcgcacgg aacagttcac
      121 gctcgcgcgc accttccgct gagttatcgg ttgccgagaa gaaccagatg ccgaggaatg
      181 ccacgctgat agagaacagc gccgggtatt catacgggaa gatggctttt tcgtgaccga
      241 ggatctgtac ccaaatagtc gggccgagga tcatcagcac cactgcggta atcaacccca
      301 gccagccgcc cagcatcgtg ccacgggtag tcagtttcga ccagtacatg gaaagcagaa
      361 tgatcgggaa gttacagctc gccgcgatgg caaacgcaag gcctaccata aaggcgatgt
      421 tctgattctc aaacagcatg ccgaggataa tcgcaatcac acccaaaatc aggacggtga
      481 ttttcgatac ccgcagttct tcacgttcgg tcgcaccttt tttgaagacg ttagcgtaca
      541 agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc cgcaaccacc gcgaggatag
      601 tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt gccgcccact gcattcgcca
      661 ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc cgccgcgtct ttatattccg
      721 gattcgcacc aaccagcatg atcgcgccga agccgataat aaaggtcaga atatagaagt
      781 agcccataaa cccggtggcg tagaacacgc tcttacgtgc ttcgcgggca tcgctgactg
      841 taaagaagcg catcagaatg tgcggcaagc ccgccgtacc aaacatcagt cccagaccca
      901 gagagagcgc ggagatcgga tctttcacca gcccgcccgg cttcatgatg tcgacacctt
      961 tcgggtgtac cgccatcgct tcgctgaaca gattattgaa gctaaagccg acgtgtttca
     1021 tcaccataaa ggccataaag ctggcaccga acagcaacag cacggcttta ataatttgca
     1081 cccaggtggt cgccagcatg ccgccgaaca ggacgtacat catcatcagc acgccgacca
     1141 gcaccaccgc aatgtgatag ttaaggccaa acagcagctc gatcagttta cctgcgccta
     1201 ccatctgggc gataaggtaa agcgccacca ccaccagaga accacaggcc gaaagaatac
     1261 gaatcggccc ttgtttcagg cggtaagagg ctacatcggc aaaggtgtag cgtcccaggt
     1321 tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca gcccaccagg aagcccagcg
     1381 agtaaattaa gccgtcatag ccggaggtaa acaccagcgc ggagatcccc aaaaatgagg
     1441 cggcggacat atagtcaccg gcaatcgcca gcccgttctg gaagcctgtg atattgccgc
     1501 ctgccgtgta gtagtcgcta cgagaacgta cgcgttttga tgcccagtag gtaatgccga
     1561 gcgtaaacac gacgaaaatc aggaacataa taatcgcctg ccagttcgtt ggctggcgct
     1621 ctacggcccc gctaatagca tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg
     1681 ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg acttcattat taagacggtc
     1741 gaattcgccg ttcgcccgcc agatgtagat accggtgaga acaaaggaga tcacaatcac
     1801 tccaacacca attggaatac cgcgtgtgac gctggtgttc ggattcagcg gcgtgcccag
     1861 ccagccgggc gcgaaggcga tcagtaaaat aaagccgata taaactgcca gcataataat
     1921 cgacaggatg gtggcaaacc gttgccgttt ttcgactaac tccctgaaat gcgcattgtc
     1981 ttctatccgc tgataaatag tgccattcat cacagattct ccagaggtaa tgtagggatt
     2041 gttttaattc ccgctccctt atgggagaag gttaacgctc gggtaaccct tgccgaatgt
     2101 aggccggata aggcgtttac gccgcatccg gcaattgtgg gttacgatgg catcgcgata
     2161 gcctgcttct cttcaagcag cttctcgact acgccaggat cggcaagcgt cgaggtatcg
     2221 cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa tacggcgcat aattttgccg
     2281 gagcgggttt taggcaggga gtcggtccag tgcagcacgt ctggcgtcgc cagcgggcca
     2341 atctctttac gcacccagtt gcggacttct gcgtacagtt ctggtgacgg ttcctccccg
     2401 tgattaagcg tgacgtaggc gtagatcgcc tgacctttaa tattgtgcgg aatacctact
     2461 acggcggctt cggcaatctt cggatgcgcc accagcgccg actcaatctc tgccgtcccc
     2521 agacggtgac cggagacgtt cagcacgtcg tccacatgcc cggtgatcca gtaatagcca
     2581 tcttcatcgc gacgcgcgcc gtcgccgctg aaatacatat ttttgaaggt ggagaagtag
     2641 gtctgttcaa aacgttcgtg atcgccaaac agcgtacgcg cctgacccgg ccaggagtcg
     2701 gtgatcacca ggctgccttc ggtagcgcct tccagcgggt taccttcgtt atcgaccagc
     2761 gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg ctttcagccc ggtagcgcca
     2821 ggcagtggcg tgatcatgaa accgccagtt tcggtctgcc accaggtatc gaccaccgga
     2881 catttctcgt tgccgatttt tttccagtac cactcccacg cttccgggtt aattggctcg
     2941 cccacggaac cgagaatgcg cagcgacgaa cggtcggtac cctcgatcgc tttatcgcct
     3001 tccgccatca gcgcgcggat cgccgtgggt gcggtataga gaatattgac ctgatgcttg
     3061 tccaccacct gcgccatacg ggcaggcgtc ggccagttgg gtacgccttc aaacatcagc
     3121 gtggtcgcac cgcaggccag cgggccgtac agcaagtaac tgtgtccggt cacccagccc
     3181 acatcggcgg tgcaccagta gatatcaccc ggatgataat caaagacata tttaaaggtc
     3241 agcgccgcgt acaccagata accgccggta gtatgcagca caccttttgg cttaccggta
     3301 gaaccggagg tgtagagaat aaacagcgga tcttcggcgt tcatctcttc cgcctggtgc
     3361 tgatcgctcg cttgctcaac caggtcgtgc caccacaggt cgcgcccttc ctgccagtca
     3421 attttcccgc cagtacgctt cagtaccacc acatgctcta cgctggtgac gttcgggttt
     3481 ttcagcgcgt catcaacgtt tttcttcagc ggaatactgc cgcccggcac gcacaccttc
     3541 gtcggaagtg atcaccagtc gtgagttgga atcaataatg cgcccggcaa cggcttccgg
     3601 cgagaagccg ccgaaaatca ccgaatgcac cgcgccaatg cgggcgcagg ccagcatcgc
     3661 aaccgcggct tccggcacca tcggcatata aatcgccacc acatcacctt ttttaatgcc
     3721 cagctcgagc agggtattgg cgaagcggca gacgtcgcgg tgcagctctt tatagctgat
     3781 atgtttgctc tggctggcgt cgtcgccttc ccagatgatg gcggtacgat cgccgttttc
     3841 ttgcagatgg cggtcaaggc agtttgccgc cagattcagc gtgccgtcct cgtaccattt
     3901 aatggacaca ttaccggggg caaaggaggt gtttttcacc ttctggtaag gtttgatcca
     3961 gtcaagaatt tttccctgtt cgccccagaa ggtatcaggt acgttaatag attgttgata
     4021 cattgcctcg tactgctgag ggtttatcag gcaacggtct gcgatgttgg caggaatggt
     4081 gtgtttgtga atttggctca tgcttttgtt ctccttgtag aatgttaata atatgtgaca
     4141 taagcgttaa atgtaggggt attggcagtt ttgtttagta tttgggcgac agatcacgca
     4201 aaagtagaat tgtgcaaata aacggcaggg taatttttga aggtcagcaa caaaagttga
     4261 ttaattcttt gaggaacatg cagttatgca tgctgttgaa aaagaggaag atactgacta
     4321 actctaaagt ggtattttac atgcacttac aattgattaa agacaacatt cacagtgtgg
     4381 ttatttgtta cacatagggg cgagcaatgt catgacagtg taggtgcggt tactgtcgtg
     4441 aaaaacaata aaaaccgcca ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa
     4501 ggataaaaat aaacgcacgc cgtatcttca gcttattgat tccttttttc tttttcactt
     4561 ctgttcacgc tgaacaaacg gcagctcccg caaaacctgt aactgtggaa gcgaagaatg
     4621 aaacctttgc cccgcagcat cccgatcaat atctctcctg gaaagccacc tcggaacagt
     4681 cagagcgtgt tgacgccctg gcggaagatc cacggctggt gatcctgtgg gcggggtatc
     4741 ccttctcgcg cgattacaac aagccgcgtg gacatgcctt tgctgtgacc gatgtgcgtg
     4801 aaaccctgcg taccggtgcg ccgaaaaatg ctgaagatgg tccgctaccg atggcgtgct
     4861 ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa agacggcgaa gatggctact
     4921 tccacggtaa gtgggcgcgc ggcggcccgg aaatcgtcaa caacttaggt tgtgccgact
     4981 gccataacac cgcctcacca gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc
     5041 cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg
     5101 accagcaatc gatggtttgc ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca
     5161 aagcggttaa attcccgtgg gatgacggca tgaaagtcga aaatatggag cagtattacg
     5221 acaaaattgc cttctctgac tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc
     5281 agcacccgga atatgaaacc tggacagcgg gcattcacgg taaaaacaac gtgacctgta
     5341 tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa actctacacc gaccataaaa
     5401 ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag
     5461 ctgccttgca aaaagtggtc gcggaacgta agcagtcgat taacgacctg aaaatcaagg
     5521 ttgaagatca actggttcac gctcacttcg aagcgaaagc agcgctggat gcaggcgcga
     5581 cggaagctga aatgaagcca attcaggacg atatccgtca tgcccagtgg cgctgggatc
     5641 tggcgatcgc ttcccacggc attcatatgc acgcaccgga agaaggttta cggatgctcg
     5701 gtacggcgat ggataaagcg gcggatgcac gcaccaaact ggcgcgcctg ctggcgacca
     5761 aaggcatcac ccatgaaatc cagatcccgg atatctcaac caaagagaaa gcccagcagg
     5821 ccattggcct gaacatggaa caaatcaagg ccgagaagca ggacttcatc aaaacggtga
     5881 tcccgcagtg ggaagaacag gcacgtaaaa acggtctgtt aagccaataa ccccgttccg
     5941 cctcgcaagg ggcggaaaac acaatggagt gaatatgagc gtattacgtt cgttattaac
     6001 tgctagggtg ctggcgtcgg gcctgttgtg gagcctgaac ggaattaccg ctacccctgc
     6061 ggcgcaggca tctggtgatc gctacgaagt tacccagcag cgtaacccgg atgccgcctg
     6121 tctggactgt cataaaccag ataccgaagg tatgcatggc aaacatgctt ccgttatcaa
     6181 cccgaataac aaattgccgg tcacctgcac caactgtcac ggccagccat caccgcaaca
     6241 ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg atgtacaagg tcggagagca
     6301 gaacagcgtc tgtatgtcct gtcatctgcc ggaacagttg caaaaagcgt tctggccgca
     6361 cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat tccctgcatc cacagcaaga
     6421 tacgatgcag acgttaagtg acaaaggacg gattaagatt tgcgtcgatt gccacagcga
     6481 tcagcgcacc aatccgaact ttaacccggc gtctgttccg ttgcttaagg agcagccatg
     6541 acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc
     6601 gcagggcgcg tggtggcgaa aacgttgaat atcaatgggg tgcgttacgg catggtgcat
     6661 gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg cttgtcgaga agtgaacaag
     6721 gtgccggaag gcgtctcgcg cctgacgatt attcgtagcg agccgcaggg cgaatttcct
     6781 gatgtgaaat atcgcttctt ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt
     6841 gacgtctgcc cgaccggtgc gtcttttcgt gatgccgcta gcgggattgt cgatgttaat
     6901 cctgatctct gcgtcggttg tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt
     6961 atccatccgg tcacgaagac ggcggacaaa tgcgatttct gccgtaaaac caatttgcag
     7021 gccggtaagc tgcccgcgtg cgttgaggct tgcccgacga aggcgctgac gtttggcaat
     7081 ctggacgatc ctaacagtga gatttcgcaa ctgctgcgcc agaagcctac ttaccgctac
     7141 aagctggcgc tgggaaccaa accgaagctg taccgcgtac cgtttaaata cggggaggtg
     7201 agccaatgac gcagacttcc gcatttcatt ttgaatcgct ggtgtgggac tggccgattg
     7261 ccatctacct gtttttgatt ggtatttctg ccggtctggt gacgctggcc gtgctgttac
     7321 gtcgcttcta cccgcaggcg ggcggtgcag acagtacgtt gctgcgcacc acgctgattg
     7381 tcgggccggg cgcggtgatc ctcggtctgt tgatcctcgt cttccacctg acaagaccgt
     7441 ggaccttctg gaagctgatg ttccactaca gttttacctc ggtgatgtcg atgggggtga
     7501 tgctgtttca gctttacatg gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg
     7561 atttgcttgc ccagcaacaa cgctggttgc cgaagctggg gatcgtgcaa aaggttctga
     7621 gcctgctgac gcccgttcat cgcggactgg aaacgctgat gctggtgttg gcggtgctgc
     7681 tgggggctta taccggcttt ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc
     7741 cgatcctgcc ggtgctgttc ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga
     7801 tcgccatggc gatacgccaa cgcagtaacc cgcattccac ggaagcgcac tttgttcacc
     7861 ggatggaaat ccccgtggta tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc
     7921 tggcgctggg cgatgacggt aaagttcgtg cgctggtggc ggcattaggt ggcggtttct
     7981 ggacgtggtg gttctggctt ggtgtcgccg ggctggggct gattgtgcca atgttgctca
     8041 aatcgtgggt caatcgcagt tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc
     8101 tggtcggcgt gttgatgctg cgctttttca ttctctacgc cggacagttg acggtggcgt
     8161 aagccagaaa agaggtggtt tctggacgta ttccttcctg aagtcggttt tctggcgttg
     8221 ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg cctttgcggg agtgcggttg
     8281 cgctggcctg ccatgatgcg actcacttgc atcggcattc tggcgcagtg cgcgctcctg
     8341 ctgctcgcct ttggcgtact gacgtattgt tttctcatca gcgatttctc ggtcatttat
     8401 gtcgcccaac atagctacag cctgctgtcg tgggaactca aactggcggc ggtgtggggc
     8461 ggtcatgaag gttcgctgct gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt
     8521 gcctggcatt atcggcagca aaccgatccg ctatttccgc tgacgctagc cgttttatct
     8581 ctcatgctcg ccgcactgct actgtttgtg gtgctgtggt ccgatccctt cgtgcggata
     8641 tttccaccag caatcgaagg ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc
     8701 tttcatccac cgctgcttta ccttggctat ggcggtttga tggtagcggc gagcgtggcg
     8761 ctggcgagtc tactgcgcgg cgagtttgat gcggccagcg cccgaatttg ctggcgctgg
     8821 gcgttacctg gctggagtgc attaacggcg gggatcatcc tcggttcctg gtgggcctac
     8881 tgcgaactcg gctggggcgg ctggtggttc tgggatccgg tggaaaatgc ctctttatta
     8941 ccctggcttt ctgccactgc gctgctgcac agtttatccc tgacacgcca gcgggggatt
     9001 tttcgccact ggtcgctgtt actggcgata gttactctga tgctgtcgct gctgggcacc
     9061 ttaattgtcc gttctggcat tctggtttcg gttcatgcgt tcgcgctgga taacgtccgc
     9121 gccgtgccgt tgttcagcct gtttgcactg attagccttg cgtctctggc tctgtatggc
     9181 tggcgagcgc gggacggtgg cccggcggtg cgtttttcgg ggttatcgcg ggaaatgtta
     9241 atcctcgcta cgctgttgct gttttgcgca gtgctactga tcgtgctggt gggaacgctt
     9301 tatccgatga tttacggtct gctgggctgg ggacgcctct ccgttggcgc gccgtatttt
     9361 aaccgcgcga cgttaccgtt tggtctgttg atgctggtgg tgattgtgct ggcgacgttt
     9421 gtctctggca aacgcgtgca gcttccggcg ctggtagctc atgcgggcgt gctgttattt
     9481 gccgctggga tcgtggtttc cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg
     9541 ggtcagcagg tgacgctggc aggatacacc ttccgttttg agcgcctcga tctgcaagcc
     9601 aaaggcaatt acaccagcga aaaagcgata gtggcactgt ttgaccatca gcaacgcatt
     9661 ggtgaactga tgccggagcg gcgtttttac gaagcacgtc gtcagcaaat gatggaaccg
     9721 tcaattcgct ggaacggcat ccatgactgg tatgtggtca tgggtgaaaa aaccggagcg
     9781 gatcgttacg cttttcgctt gtatgtacaa agcggtgtgc gctggatctg ggggggagga
     9841 ttgttgatga ttgcgggcgc attgttaagc ggatggcggg ggaggaagcg cgatgaataa
     9901 aggacttctc acgctactgc tgttatttac ctgttttgcg cgcgctcagg tcgtagacac
     9961 ctggcaattc gccaatccgc aacaacagca acaggcgtta aatattgcca gccagttacg
    10021 ttgtccgcag tgccagaatc aaaacttact ggaatccaac gcgccggtgg cagtcagtat
    10081 gcgccatcag gtttacagca tggtggcgga ggggaagaac gaagtcgaaa tcattggctg
    10141 gatgaccgaa cgctacggag attttgttcg ctataaccca ccgttaacgg gtcagacgct
    10201 ggtgttgtgg gcgctgccag tggtgttgtt actgctgatg gcactgatcc tctggcgagt
    10261 gagggcgaag cgatgaaaga gcccaaaata ccggtgaaaa tgctgacaac actcacgatt
    10321 ttgatggtat ttctctgtgt cggcagttat ctgttaagtc caaaatggca ggcggtacat
    10381 gcggaatatc agcgtcagcg cgatccgcta catcagtttg ccagccagca aaccccggaa
    10441 gcgcagcttc aggcattgca ggataaaatc cgcgctaatc cacaaaacag cgaacaatgg
    10501 gcgttactgg gcgagtacta tctgtggcaa aacgattaca gcaattcgct gctggcgtac
    10561 cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt atgtggcgct ggcgacggtg
    10621 ctttattacc aggccagcca gcatatgacc gcccagactc gcgcaatgat cgacaaagcc
    10681 ctcgcgctgg actctaacga aatcaccgct ctgatgctgc tggcttccga tgcgtttatg
    10741 caggcgaatt acgcgcaagc catcgaacta tggcaaaaag tgatggatct caactcaccc
    10801 cggatcaacc ggacacagct ggtcgagtcg attaatatgg cgaaattgtt gcagcggaga
    10861 tcggattaat catctggtga tgaagattat gttattgagc tgcataaaaa ataatcgaat
    10921 gaacatatgc caaaaataat cactaatcag tattattgca gattaaacaa ataaaaatct
    10981 ttccataaca aatggttatt cattaatcct gacataaact cgcgttatta tgcattaatg
    11041 cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc gcgcaaatga tactgatcat
    11101 aagcgttaaa aaaatctaca aaccaacgca acacaattca tgcctggcag tatgtcacgt
    11161 tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa gttattctga tgaaaaatat
    11221 aaaattcagc ctggcctggc agattctgtt tgctatggtg ctgggcattc tcctgggaag
    11281 ctacctgcac taccatagcg acagccgcga ctggctggtc gtcaatttgc tctctccggc
    11341 gggtgatatc ttcatccatc tgattaaaat gattgttgtg ccgattgtga tctccacgct
    11401 ggtggtgggt atcgcgggtg tgggtgatgc caaacagctc gggcgtattg gcgcgaaaac
    11461 cattatttac ttcgaggtga tcaccaccgt cgccatcatt ttggggatca ctctggcgaa
    11521 cgtcttccag cccggtgccg gggtggatat gtcgcagctg gcgaccgtcg atatctcgaa
    11581 atatcagagc actacggaag cggtacaaag cagttcccac ggcattatgg gcacgatttt
    11641 gtcgctggtg ccgacgaaca ttgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat
    11701 cttcttctcg gtgctgtttg gtctggggct ttcctccctg cccgcgacgc atcgtgaacc
    11761 gctggtgacc gtgttccgct ccatctctga aaccatgttt aaagtgactc acatggtgat
    11821 gcgttatgca ccggtgggtg tgtttgcgct gattgcggtg acggtggcta actttggttt
    11881 ctcttctctg tggccactgg cgaaactggt gctgctggtg catttcgcca ttctgttctt
    11941 cgcgctggta gtgctgggaa ttgtggcgcg cctgtgcggg ttaagcgtct ggatcctgat
    12001 tcgtattctg aaagatgagc tgattctggc gtactccact gccagctctg aaagcgtact
    12061 gccgcgaatt attgagaaga tggaagccta cggagcaccg gcgtcgatca ccagcttcgt
    12121 ggtgccgacc ggttactctt ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc
    12181 tattttcatc gctcagctgt atggcattga cctgtccatc tggcaggaaa tcattctggt
    12241 gctgacgctg atggtgacct cgaaggggat tgctggcgtg ccgggcgtgt cgtttgtggt
    12301 gttgctggca acgctgggta gcgtaggtat tccgctggaa ggtctggcgt ttattgctgg
    12361 tgttgaccgt atcctcgaca tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc
    12421 ggtgctggtg attgccaagt gggaacacaa atttgaccgc aaaaaggcgc tggcttatga
    12481 gcgtgaagtg ctgggcaaat ttgataaaac tgcggatcaa taattgaaga ttgccggggt
    12541 tatccacccg gcaatgtgtg gatgcctgat gcgacgctgg tgcgtcttat caggcctacg
    12601 ccagacagcg caatagcctg atttagcgtg attttgtagg tcggataagg cgtttacgcc
    12661 gcatccgaca tcaacgcctg atgcgacgct taacgcgtct tatcaggcct accttaaccg
    12721 ttggttaatt tttcaaactc ttcacacccg gtatcaaacc cttccataca gctcaggttc
    12781 aaccagtgca gcgccttttg tttgttcttc tcgataaaac cttcttcacc gtttaagaac
    12841 atcattcccg cccagtactc ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa
    12901 tactaggttg ccttgtcatc gtcggcctta atacccacgc cgttggcgta aatcaaacca
    12961 agcagcattt gggcatcgac ggcagagtcg ttctccagat cttccgaggc ttttctaaca
    13021 gcgaaatcgc ctttggataa tccggtttac ccgcttgagt atttaccaga atatgcgcca
    13081 gcgttacttc accttcttta ctgcccgctt gcgccgcttt ctctgcaaga acttttgctt
    13141 gtggatagtc cagactgccc ggattggtga ttttgatctg cgccaacagc gcgcaggcat
    13201 cagcatcacc gctgtcggcg gctttctgtg cccaatattc ggctttgctc aaatcgccgg
    13261 agctaaacca gctgtcggca agaaaatatt gtgcgcgtcg gtccccggcc tctgctgcct
    13321 ttaaatactg gctgccaggc tcggagtcgt tggcgtgggc aaagaatgtc aaaaacaaca
    13381 tcaatgcgat aatttttttc attttgataa tacgattggg tagggagtaa ccagtataaa
    13441 gagttcgaat ggataaaaaa acagcctccg aaaggaggct gtagaaagga ctgtattacg
    13501 ccagtgccgc ttcgcgcagg cgagttttca acttgttgta ctcgtcaatc acgtactgct
    13561 cggcggcgcg ctgatcggcg atcggctcga cgcgcacggc gcagtatttg tattccggcg
    13621 ttttcgtaat cgggcttaag ttttcggtaa ccagctcgtt acaggcacca atccaccact
    13681 ggtaggtcat gtaaatcgcc cctttgttcg gacgatcgct gacctgcgca cgggtgataa
    13741 ttttgccttt acgcgagtgc acccaaacca atgcctcatc ttcaataccc agacgtttgg
    13801 cgtcttcggt attgatttgt gcgtagccag gttcatcagc cagcgccgcc agtgccgcac
    13861 agttaccggt catcgaacgg caagagtagt gaccaacttc acgcaccgtt gacagtacca
    13921 tcgggtactc gtcggtgagt ttgtcgattg gcgctaccca gtcgcaggtg aagaactgcg
    13981 ccagaccgtt cggggtatca aacttctctt taaacagata agaagtcccc tgatcggcat
    14041 ctgaagtatc gcggcaaggc cactgaatga agccctgttc gcccattttc tcgtaagtcg
    14101 caccgtagaa atccgggcac agatgacgca actcatccca gatctcctgg gtgttgttgt
    14161 agtgcatcgg ataacccata cgggtggcga tttcactgat gatttgccag tccgttttca
    14221 gatcccattt cggttcaact gctttgaaga aacgctggaa gccacggtca gccgcagtaa
    14281 acacgccttc atgctcgccc cacgacgttg acggcaaaat gacatccgcc gccgacgcgg
    14341 ttttggtcat aaagatgtcc tgaacgatga ccagttccag atcttcaaag gctttacgta
    14401 ctgccgacag ctccgcatca gtttgcagcg gatcttcgcc cataatgtac gcggcacgca
    14461 cttcgccatg cgctgcgcgg tgcggcagct cgctgatgcg atagccggta tgagcaggca
    14521 ggctttccac gccccaggct ttggcgaatt tctcgcggtt agccggatct ttcacgtact
    14581 ggtatcccgg atacgtatcc ggcagcgcgc ccatatcgca ggcaccctgc acgttgttct
    14641 gaccacgaac cgggtttaca cccgcatgcg gcttaccgag gttaccggtc agcatcgcga
    14701 ggctggtcag agaacgcacg gtttccacgc cctggtagaa ctgggttaca cccatgcccc
    14761 acaggatggc ggcgcttttc gcctgggcat acatccgtgc cgcctgacga atctcactgg
    14821 cgctgacgcc ggtgatatct tcaaccgact ccggcgtgta gccttcaacg attttacgat
    14881 actcttcaaa gccttctgta cgggaagcga cgaacgcttt gtcgtacaga ttttcttcaa
    14941 taatgacatg gcccatcgca ttcaacagcg cgatgttcga gccgtttttc agtgcaatgt
    15001 gcatgtcagc aatgcgcgcg gtttcaattt tgcgcggatc gcagacgata attttcgccc
    15061 cgttacgttt agcgttaatt acgtgattcg ccatgattgg gtgggaatcc gccgggttgt
    15121 acccgaaaac gaacactaaa tcggtattat caatttcgtt aatagcattg ctcattgcgc
    15181 cattaccgac cgattggtgc agacctgcaa ccgatgggcc gtgtcagaca cgagcgcagc
    15241 agtcaacgtt attggtacca ataacggcgc gcgcaaattt ttgcattaca tagttggttt
    15301 cgttacccgt accacgcgag gagccggtcg tctggatggc atccggaccg tacttctctt
    15361 tgatggcgct caggcgctcg gcaacgtaat tcagtgcctc atcccaggaa acaggttcga
    15421 gtttgccgcc acgctggcga cggatcatgg gggttttcag gcgcggggtc aggatctggg
    15481 tatcgttaat gaagtcccag ccataataac ccttcagaca cagggtaccc tggttggttt
    15541 tcccctgcgc tgcctccgcc cggacgattt tgccgttatc gacgaccagg ttgattttgc
    15601 aacctgatgc gcaatagggg caaaccgtga cgactttttt catcggtctc gctccagtta
    15661 atcaaatcac gcatacgcgc tctcgactac agtatgcatc ttttatgcca cattttattt
    15721 ggggtcattc cctgatatta cgggcactat ttattcaaaa ctctgacgaa aaacaggctg
    15781 tcgtcagttt tgacgtgacg aaacgaaata ccgcgtgaca gccatcacgc ggcagacatt
    15841 ttattttttc tcgacgacgg gagccgcctg atacccgccg cccagcgatt tcatcaactg
    15901 aatgctgtgg atcacccggc ggctgtccag catcagtaat gacatctctt cggcaagcac
    15961 tggcaaccgg gcttcggtgg cctgtaagcg gctggttaag ccgcgctgat aggcggcctc
    16021 ggcagcgcgc tgggtgaagc gcgttgcttc cacgcgttca gcctgcattt ctcgttcgtc
    16081 gttgagcgtt tgcagacgcg tgccgttgac ggcaacgtca cgcaccgcgt tcagtactga
    16141 ctggttgtaa cgttcaatca tcatgttgct ggcggcgcga gtgccttcga gattggcatt
    16201 caaccgtcca ccgtcaaaca gcggcaattt cagacccggg atgaagttga actggcgact
    16261 ggtttttttg aataaggtat ccaggtggat ggagtccaga ccgaaaaacg ctttgatatc
    16321 aaagctcggg tagaacagtg cccgcgcgga atccacctga tctaatgacg cctgaacata
    16381 ccagcgcatg gcttgcagat ccgggcgtct ggcgagcaac tcataagaga gtgtcgccgg
    16441 aatgccggtc tggactcgtg gtaatgccac cggtttgatc tccggcatat cgctggcccc
    16501 cgcgccaatc aatgcacgca gagattctcg cgtttcggtg atttgccctt tgacggcagc
    16561 aatttgttta tcgaccgcca gaatctgtgc ccgcgcgccg tggaaaggca cttgtgcttc
    16621 cagaccgtgc gccactttac tctggtgcgc tttcaccgcg taatcaatca catcgcgagt
    16681 ttgttctaac agatcgagca tctgatagct ggcctgcata ctgtaataaa gctgcgctat
    16741 gcccgtggtc agcgatagct ctactgctgc ggtttctgcc agcgcggcat tatgcgcgcc
    16801 aatggcggcg gcaaccgctg agcgatgcac accccacaaa tcaagatcca gtccggcaaa
    16861 caaacctact gtggcttccg tatagtacgg cccgtccata cccagtgagg gcgcatccat
    16921 cgcataaggg cttaaaaagc cgttcgccga gacgcgttgg cggttgagca tccctaacgc
    16981 tgcgacctgt aattgtgaac cggcatctaa caaatcggcc tgggactgcg ctttttcttc
    17041 ccgcagtttc gcttcggcga gggtgtgtga accacttagc gtccgttgga tcagcgcatc
    17101 cagctgcggg tcattgagtt gtttccacca ctgcgcctgc ggccagccgg agctggcaag
    17161 atgaatatcg tcggccagtt tgatttgttc cggtttgagc tgttgatgag gtgcagaatc
    17221 cttacgtacc agggcacagc cggaaatcag cgtcgtgctg ccgagaatgc tgcacaacag
    17281 cagacgtgaa agttgacgat tgatcattgc gtggctcctt gtgcctgttc cgtggcgggc
    17341 gttaatgccg gggctgtcca gtctggcaag cgggcgaaaa gctggcagac gtgttgctcc
    17401 tgttttaata aggtgggcag gatggcctgc gaggcgggtg tctcttcaag cgttatttga
    17461 ggagaacagc tgagtgcggt tgccagaccg gcagcgtatt tttccagggc gtcggcaaac
    17521 tgacctgctc tgtccggctg tagtgcgtta tccagcgcct gcgccgagtt ccaggtggca
    17581 tcccaggcgt gaagaagatc gcggccctga cgaataaccg tttgcgaacg ttcaatcagt
    17641 aaggcgcgtt cttcgctgtc cagttgacgc tccagcgcca cgcgttggca catctcttca
    17701 caggcattaa acgccgcatg cagacctata cgaatttgca gataagtgcg cagagccgtg
    17761 acttcctgct ggcgtgggat ccgcattact ttacttagca tacccagcgc accggctagt
    17821 ttttgcggca gagtgcgcgc ttcactttca ggccagacaa aggtgtaaat caccgcggac
    17881 accacggtac caatgatgat acccagggcg cgatcgcgaa tttccaccag gtcgtacact
    17941 gggccaaaaa cgttttcgag cgtggcgagc gcgaaggtga cgaccatctg tgtgccgata
    18001 taagaagagc gttcagagct ggtggcaatc catgcgccca acaggaaaat cggtgccagc
    18061 acaaacagca attcgacaat attgtccagc cagggcatga ccagtagcgt gaataacagc
    18121 gccagaatcg cgccgcaaaa ggccccgcca aaacgcagca ccatcttctg gtacgacgaa
    18181 ccgacatttg gattagcgac gatcacgcag gtcagcatac aggtgtgaat gccttcccag
    18241 tccacgccgc tgtaaaaggt gtaacagatc aaacaggcga gcaatgtttt taccgcgtag
    18301 cgtatatagt ctggattggt aaaagcatcg gcgaccattg atggcggttt ggcggcgggc
    18361 gttggcggcg tattcgggtt catctggccc agttgtaaca acgtctggca gatattctcc
    18421 aggttacatt cccgtgccgc caccgcttca ctttcactga tccgccagtc gctttgccag
    18481 cactgacctt cagcaacggc atgctgcagc ttgttgattt ctgaggctaa tttttgccga
    18541 aattcaataa ttgcctgaga atcagcaaaa gaggtgggat cgtagcgatt cagcgtcgag
    18601 taaatgtagg ttaccgttgc cacgcagctt tgccaccatg cgctttgagt tcgccagttg
    18661 gcatcgtccg cgagacaaaa gacattcagt ttttgcagcg ccagcgcctc tctttcaatc
    18721 cgcgtttcgg gtagcggtgc gaggctgtct gtcaggtgac taatggcatc atcaagccga
    18781 tcattaagcg cctgatgcat ttgcgaaatg gcacgactgg gaaaccacag cacgccgatt
    18841 aacgtcatca gcaaggttgg atagaggcca acaacgatac accacagcgt taagcgcacg
    18901 accacttccg gatagtcgag catggcgggg aaggtttgcc cgtaaatggc gacaatggcg
    18961 acagcgaaaa agaccagtcc cagccgatgg gtgcgcatca aaaacatgca gcccatcagg
    19021 atcggcccgg cgatgatcaa tcggatcaac ggttcgccgt atgaccattt gtagatcaaa
    19081 aacaggctgc cgatctccag caccgtggca accacaaaca agatcgccac aaatttggtg
    19141 taaaacgcgt tcgactgaat accgtaaaac agcactgcca gcgataacgc cacaaaaggg
    19201 atctcaaaag tcatcgagat cagaatcacc agcaggcagc ctacccagag ttgcacgatc
    19261 tgcggcacgc ggccgggtcg ccgctcgctt aactcttcat gaaagaacgc cagcagcctg
    19321 accaccggta atggcaggga gttgagcgcg ctcattattg cggctcaaga ttagcgactg
    19381 ccgaagcgcc gatgcggaac atttccgggt caggtttatc gaccatgatt ttgaccggaa
    19441 aacgctgggc aacgcggacc cagttaatag agcgagaaac tttcggcagg ccgcccagca
    19501 ccaggccgcc gtcattcggt agcacaccgt agccaatcga atccacttta ccctcgaagg
    19561 ttttgccgct gtcactcatc aggcgaatcg ttgcgggtgt acctgagcga atatttttca
    19621 gatcggtttc gcggaagttg gcgatcacgt accagtgacg agtgtcgatt agggtaaaaa
    19681 tagggcgcat ggcagaagca aattgcccga cggaggtttt gagggaaatg acccggccat
    19741 caaacggcgc gcgaacggtc gccatttcca gatgcagttt ggtcagggca atatccgctt
    19801 cgaccgccgc acgctgggca actaatacat ccacgccgct gacggcgctg gcggctgact
    19861 gcgcctgtaa caatacggca ttaagatccg cttctgcggc gcgctgcgcc gttcttgcac
    19921 ggtcaacatc ttccgctgag acaaaacctt ctttcagtaa tggctcggtg cggcgtaatg
    19981 tatctgtggc ctgtttcgcg gcggcacgtg ctttttctac cgtggcatta accgagtcgg
    20041 caccaaactg ttgggcgtca acgctacgct gggtgagcat aatttgctta tccagcgccg
    20101 cgagggaggc ttcagctttc gccagattgg cttcgtacgg gcgcgggtca atgcggaaca
    20161 gcaaatcgcc ctgtttgact gcctggttgt cggtgaccgc cagttctaca atgcggccgc
    20221 tgacttccgg caccacatca atggtatctg ctgacgcgta agcgtcatta gttgatggcg
    20281 cactgtctac gcgccagata acgaaaacaa gggcaaccag cgccaacgca accactaaca
    20341 gagcagggaa tttactgcga ggagcttttt tcggcgtact ttccatgagc gcgtctcaaa
    20401 tagattagaa aaatgccagc cagaacagca tggcgtagac agcaaaaagg gcggtataaa
    20461 taattccgac aaatgccaga ttgatatttg cccgctgaat aattcgcctc gtgattagcg
    20521 tcaaaataag gctggcaatg gcgcagaaaa accagctggg ataataagcg ccgatcaccg
    20581 ggattgccgg agataatgag catcccgaaa ccatgaagac gggaattatc caggctgttt
    20641 ttttgagttg catcgccatg cgagagagaa ctgttggcat attaaataca tcctgtttgc
    20701 tgcgaacaat atctgtagtg aatatcaata tccattgaag gcgtcagtat ggctattttc
    20761 ttgatgaata aaaatagctt tattcagcca ccgcattcat aacttatata ttttatgtgt
    20821 taattgattg tatgtatttt tataataaat gcttttgggg gcttaagcgc ccccaaaagt
    20881 gagtggcatt aatttggggt tacgatattc acccacaaat cgaaattatc cagacaggca
    20941 ataatttctt ccagtttcgc gccattgcca ataattttgg ctttcttcgc ttttaccaga
    21001 tccgccattt tggcttgtcc tgtcagcacg gcgtgcagat cttcacggct gatgtagaaa
    21061 gaggcgtcgg cttgcggttg cagtgtttta cggtagttaa gcacgctatc gttcagcgtc
    21121 aggttgaggt tatcgccgtt gctcatattg aagttcaggc tgatattttt acccgcagct
    21181 ttcgcgctat cgaggcgaac ggacataaag tcgaacagca tttcgaccga catcccgcga
    21241 atggtgtccg gggaaccggt ggtgccgtgg ctgaacttat gcaccccttc gcgcagctct
    21301 ttcgcgccgg tcaggtagaa accgcgccag gtggcggact cggcctgata gcccagctgt
    21361 tcaaagttat tcgcttgcag attcttcgcg acctggtcac ccgggttggc ggcaatcacc
    21421 tgtttcagca gttctgccga ccagcggtaa tcaccttgct tgttcgcttc ttgcgccagg
    21481 ctgatgacac gggcagaacc gcccagcgcc tgcacgtaac gtttacccat ctccacctga
    21541 ccatacggat gcaggttagc cgggttaccg tcgtaatagc caagatagaa gttatacacc
    21601 gcgcgggcgt tgtggctgac agaaccgtaa tagccacggc tggcccagtt attggcaagt
    21661 gcaggcggca gtttaatcat gtcgccgatt tcattcatgg tgtagccctg gttcgccagg
    21721 tgcagggtct ggtcgtgaat gtacttgata gtatcgcgat atttaccaat ataatcattg
    21781 atatgcttat tgccccggac cggccaggtg tgcggcataa acaggacttc cgcgtcgtta
    21841 ccccacatat ccagcgtttc gttcagatac tcggtccact tgctggtgtc gcgggttttc
    21901 gcgccgcgca gagtgtagaa gttgtgcagg gtatgcgtgg cgttctcggc ggtacacagc
    21961 gctttcaggg ccggaatata gaagtgcatt tcggctggtg cttcgctacc tggggtcatc
    22021 aggaagtcaa actccaggcc gtcgataatc atcttctcac cggttttggt gatggttttg
    22081 ttcggtgcga tgatgctcgg gtagcccgat gccagcgtta cgccaagacc attgccgatg
    22141 ttaccctgag ggttatgcgc cagcagcagg ccgtaggagt aaagtgcacg gtggctcata
    22201 atattacccg ccagtacgtt ttcgctgatg gcttcgtcca taaagccagc aggggcgata
    22261 acctgcactt taccggattt cacatcagct tcagagataa tgcctttcac gccgccatag
    22321 tggtcggtat ggctgtgggt ataaataacc gcaacaatcg gtttttgcgg gcgattttgg
    22381 aagtaaaggt caagggcggc ttttgcgctc ggtggcgtca ccagcgggtc gatgacaata
    22441 atcccggtat caccttcgat aaaggtgatg ttggagatat cctgaccgcg aacctggtac
    22501 attctgtcgg tgactttaaa caggccggaa ataccgttta actgcgactg acgccacagg
    22561 ctagggttaa cggtttcggg cgcgggcgca ttgatatcaa atttataatc ttccccgcga
//
LOCUS       DAJTSL010000057        22458 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTSL010000057 DAJTSL010000000
VERSION     DAJTSL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.057"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            41..232
                     /locus_tag="OO433_001956"
     CDS             41..232
                     /locus_tag="OO433_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6050449.1"
                     /translation="MKYGKLGYIVPRPQKIRGRWLIDRRAVFVGPGETGIAPEIHTGD
                     DDALKEILTHVTEATKKQH"
     gene            201..1307
                     /locus_tag="OO433_001957"
     CDS             201..1307
                     /locus_tag="OO433_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6050450.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWV"
     gene            1487..2281
                     /gene="potC"
                     /locus_tag="OO433_001958"
     CDS             1487..2281
                     /gene="potC"
                     /locus_tag="OO433_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6050451.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMISPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDETKGNTGDVK"
     gene            2278..3324
                     /gene="potD"
                     /locus_tag="OO433_001959"
     CDS             2278..3324
                     /gene="potD"
                     /locus_tag="OO433_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6050452.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETCIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3480..4301)
                     /gene="cobB"
                     /locus_tag="OO433_001960"
     CDS             complement(3480..4301)
                     /gene="cobB"
                     /locus_tag="OO433_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6050453.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     KIQPNAAHLALAKLQEALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4317..5228)
                     /gene="nagK"
                     /locus_tag="OO433_001961"
     CDS             complement(4317..5228)
                     /gene="nagK"
                     /locus_tag="OO433_001961"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6050454.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQQLQAPEIIALYNQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5257..6501)
                     /gene="lolE"
                     /locus_tag="OO433_001962"
     CDS             complement(5257..6501)
                     /gene="lolE"
                     /locus_tag="OO433_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6050455.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEMTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     LIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6501..7202)
                     /gene="lolD"
                     /locus_tag="OO433_001963"
     CDS             complement(6501..7202)
                     /gene="lolD"
                     /locus_tag="OO433_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6050456.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7195..8394)
                     /gene="lolC"
                     /locus_tag="OO433_001964"
     CDS             complement(7195..8394)
                     /gene="lolC"
                     /locus_tag="OO433_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6050457.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEDSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMIFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPMAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8656..9729
                     /locus_tag="OO433_001965"
     CDS             8656..9729
                     /locus_tag="OO433_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6050458.1"
                     /translation="MKQKELWINQIKGLCICPVVIYHSVITFYPHMTTFPHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9857..13303
                     /gene="mfd"
                     /locus_tag="OO433_001966"
     CDS             9857..13303
                     /gene="mfd"
                     /locus_tag="OO433_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6050459.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTKHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13447..14409
                     /gene="ldtC"
                     /locus_tag="OO433_001967"
     CDS             13447..14409
                     /gene="ldtC"
                     /locus_tag="OO433_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR6050460.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     TRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14492..14749)
                     /gene="bhsA"
                     /locus_tag="OO433_001968"
     CDS             complement(14492..14749)
                     /gene="bhsA"
                     /locus_tag="OO433_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR6050461.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISAKAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            14990..15622
                     /gene="comR"
                     /locus_tag="OO433_001969"
     CDS             14990..15622
                     /gene="comR"
                     /locus_tag="OO433_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6050462.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15684..16223)
                     /locus_tag="OO433_001970"
     CDS             complement(15684..16223)
                     /locus_tag="OO433_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6050463.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(16450..17754)
                     /gene="ndh"
                     /locus_tag="OO433_001971"
     CDS             complement(16450..17754)
                     /gene="ndh"
                     /locus_tag="OO433_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6050464.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTHDPDIYAIGDCASCTRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(18154..18696)
                     /locus_tag="OO433_001972"
     CDS             complement(18154..18696)
                     /locus_tag="OO433_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6050465.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18719..19744)
                     /gene="nagZ"
                     /locus_tag="OO433_001973"
     CDS             complement(18719..19744)
                     /gene="nagZ"
                     /locus_tag="OO433_001973"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6050466.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKASSTRLNQLHERW
                     QEEKAGH"
     gene            complement(19755..20579)
                     /gene="thiK"
                     /locus_tag="OO433_001974"
     CDS             complement(19755..20579)
                     /gene="thiK"
                     /locus_tag="OO433_001974"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6050467.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDTALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(20563..21201)
                     /gene="lpoB"
                     /locus_tag="OO433_001975"
     CDS             complement(20563..21201)
                     /gene="lpoB"
                     /locus_tag="OO433_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6050468.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPIVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQ"
     gene            complement(21215..21592)
                     /locus_tag="OO433_001976"
     CDS             complement(21215..21592)
                     /locus_tag="OO433_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6050469.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQKPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(21595..21954)
                     /gene="hinT"
                     /locus_tag="OO433_001977"
     CDS             complement(21595..21954)
                     /gene="hinT"
                     /locus_tag="OO433_001977"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6050470.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVVAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
BASE COUNT         5593 a         5894 c         5578 g         5393 t
ORIGIN      
        1 cgacgagtgg aaaccaaaac ggttcagtct cactaccctg gtgaaatacg gaaaactagg
       61 atacatcgtt ccaagaccac aaaaaattcg tgggcgttgg ctgatagatc gccgagcagt
      121 atttgttggg ccaggtgaaa cgggaattgc gccggaaatt catactggcg atgatgatgc
      181 actgaaggag attttaactc atgtcaccga ggccacgaaa aaacagcact gacgtagccg
      241 gtctttacga aaagtttgat cgcagaactg gcagagttta ctaccagtat aaaaatcctg
      301 tgactggaaa atttcacgga ctcggaacag acaaaggtaa ggcagaaaaa atcgcttcca
      361 cagccaatca gcgaatagct gcagcagaag ctgaatattt catgcgcaaa attgatgaaa
      421 gtccgtcagc aacaaaacgt cggggtatca gattaaaggc atgggttgat cgatatctga
      481 aaatacagga cacgcgactg aaaaatggag atattgcagc tacaactcac aaagaaaaaa
      541 ctcgaatggc tgcatacctg gtttcccgtc tgggaaacca cccattgaaa gaactggaag
      601 taagagactt tgcattaata ctggatgagt ggctggataa agacatggtc agcacagcga
      661 gagtaaatcg tggattatgg gttgatattt ataaagaagc acagcatgca ggggaagttc
      721 ctcctggatg gaatcctccg gaggctaccc gtaaaccgat ccctaaagta accagagcca
      781 ggctcaccat ggaagactgg caaaaaattt acaatgcaac gcctgaaaaa cactttatcc
      841 gtaacgcaat gcttcttgcg attgttactg gtcagcgccg tgatgacatt tgccacatgc
      901 gtttttcaga tgtgtggaac gaacacttgc atatcaccca gggaaaaacc ggaatgcgtc
      961 tggcgttacc gcttacacta cgctgtgatg ccattgggat aacgttaaaa gaagttattg
     1021 atgggtgccg agacagaata ttaagtccat atctaatcca tagtcggcac cagaaacaac
     1081 cgaagccgat gagtaaagac aacctgagcg actactttgc caaagcacgg gatctggctg
     1141 ggataattcc accagcagga aaaactccgc caacatttca tgaacaacgc tctctatcag
     1201 aacggctgta ccgtgcacag ggtatcgata caaaaacatt actaggacat aaagtccagg
     1261 caaccaccga tcgctataac gatactcgag gtcaggaatg ggtttaagct ggttatttga
     1321 caaacaccat atggaatatc cgtcaccatc tcaggaaaaa agtgttgtaa aatgcgtggc
     1381 ctagttttgg ggaaacgttt tggagagatt ttggagaaag gaaaaaattg aataaattca
     1441 aaatcctgaa agtgctccac cttatgaata agaaggtgga actcgaatga tcggtcgact
     1501 gcttcgcggc ggttttatga ccgctatcta cgcgtacctg tatatcccaa tcattatttt
     1561 gattgtgaac tcctttaaca gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa
     1621 atggtatagc ctgctgatga acaacgacag cctgttacag gcagcgcagc attcactaac
     1681 aatggcggtg ttttcggcga cgtttgctac gcttatcggt tcactgacgg cagttgcgct
     1741 gtaccgttat cgctttcgcg gtaagccgtt cgttagcgga atgctgtttg tggtaatgat
     1801 atcaccagat atcgtgatgg caatttcgct gctggtgctg tttatgctgc tgggtattca
     1861 gcttggcttc tggtcgctgc tgttctcgca tatcaccttc tgcctgccat ttgtggtggt
     1921 gacggtgtat tcgcgcctga aaggttttga cgtgcggatg ctggaagcgg cgaaagatct
     1981 cggtgccagc gaatttacaa ttctgcgaaa aatcatactg ccactggcaa tgccagcggt
     2041 ggcagcgggc tgggtgttaa gctttaccct gtcgatggac gatgtggtag tttcttcgtt
     2101 cgtcaccgga ccaagttatg aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg
     2161 cgtatcgccg gaagttaacg cgctggcaac catattactg gtgctgtcgc tggtgatggt
     2221 tattgccagt cagcttattg ctcgtgatga aacgaaaggt aacacagggg acgttaaatg
     2281 aaaaaatggt cacgccacct gctcgcggcg ggtgctctgg cactgggcat gagcgccgct
     2341 cacgccgatg acaacaacac gctgtatttc tacaactgga ccgagtacgt gccgccagga
     2401 ctgcttgaac agttcaccaa agaaacctgt attaaggtta tctattcgac ttacgagtcg
     2461 aacgaaacca tgtacgccaa gctgaaaacg tacaaagacg gtgcgtatga cctggtggtt
     2521 ccttcaacct attacgtcga taaaatgcgt aaagaaggga tgatccagaa gatcgacaag
     2581 tcgaagttaa cgaacttcag caatctcgat ccagacatgc tcaacaagcc ttttgacccg
     2641 aataacgact actccattcc gtatatctgg ggggcgacgg cgattggtgt taacggtgat
     2701 gcggtggatc cgaaatctgt caccagctgg gccgatctgt ggaagccaga gtacaaaggc
     2761 agcctgctgt tgaccgacga tgcccgtgaa gtgttccaga tggcgctgcg taagctgggc
     2821 tattccggta acaccaccga tccgaaagag attgaagctg catataacga gctgaaaaaa
     2881 ctgatgccaa acgtcgcagc gtttaactcc gataacccgg ctaacccgta catggaaggc
     2941 gaagttaacc tcggcatgat ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg
     3001 cctattgacg tggtgtggcc gaaagaaggc ggcatattct ggatggacag cctggcgatc
     3061 ccggcaaatg ccaaaaacaa agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg
     3121 gatgtggcaa aacaggttgc tgaaactatc ggttatccaa cgccaaacct tgcggcgcgt
     3181 aagctgttaa gtccagaagt ggcgaacgat aaaacgctct acccggatgc tgaaaccatt
     3241 aaaaatggcg aatggcagaa tgacgttggc gcagccagca gcatttatga agagtattat
     3301 cagaagctga aagcaggacg ttaattcagc aagcgggcgg tggtattacc gcccgttttt
     3361 tatttagcaa ggatgtggct aatgagtcag gacagtagag ttttatccga ctctttcgtc
     3421 ggtattgtgg tccgctcgta ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt
     3481 tataatccct tcagcaactt ttcaacaaac tcaggcacca cctggcttgc cgggccgtaa
     3541 tatttctcgg caaattcatt accgacctga cttggttcga gattcagttc cacggtgtgc
     3601 gcgccatgca gtttcgcttc gtgaacaaac ccagccgccg gataaacatg cccggaagta
     3661 ccaatggcaa tgaaaatatc agccatcgac aacgccatat aaatttcatc catgccgagt
     3721 ggcatttcgc caaaccaaac gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg
     3781 catttatctt ctggcgtaac gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa
     3841 cgaactttca gcaattcccc atgcatatga atgacattgg tattacctgc gcgttcatgc
     3901 aggttgtcta tattctgcgt taccagcaaa aagcgatcgc cgagagcctc ttgcagttta
     3961 gccagcgcaa gatgcgcggc gttaggctga atttttggct gctgcagctg tcgacgacgg
     4021 gcgttataaa acgcttgcac cagttcagga tcgcgatcga aaccttccgg cgttgccaca
     4081 tcttcaaccc gatgttcttc ccacaggcca tctgcggcgc gaaaggtacg aatacctgat
     4141 tccgcagaaa ttcctgcccc tgtcagtacg agtactcttg gtttttccat tgcttccggc
     4201 accactttat ctctgaaaaa aatacgctga cgcaaacgct cgcgcaggcg gcgtttattt
     4261 ttacgaaaac gacttaaccg atgaccccga cgcgacagca tagcaacctc tgttgtttaa
     4321 tcggttagat gtaggaaggc cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt
     4381 tcaatgcgcg gaacacgagc tacaggtaag agatgacgag gcagcctgtc cgccagttgc
     4441 gttgtgattg ccgggaaatt cgataagcca ccaccaatga cgaccaggtc agggtcaaca
     4501 atggtcagga tatttcccag acaaaccgct aataaatcca gataacgctc aacgtgcgcc
     4561 cttgcctgct catcgccttg attataaagc gcaataattt cgggagcctg caactgttga
     4621 tgataatagt gttgatacag ccacgcaaaa ccgcgaccag acagataatt ctcaatgcag
     4681 ccatgctgac cacagccgca gcggcgtaac gggaaatcca accccatcat ggttaacgca
     4741 tcaaccggca gacgcatatg gccaaactcg ccggtaatgt agcttttacc ggtaatcggt
     4801 ttgccgttga aaatcagccc accgccaacg ccggtaccaa gaatcaaccc catcaccagt
     4861 ggatattgcg taaattcatc atcccaggct tctgaaaggg caaaacagtt ggcatcgtta
     4921 tcaaggcgta catcgcgatc aagacgtgcg ctcaggtcgg cacgcagcgg tttaccgctg
     4981 gcagcgggga cattggcggc atacagcgta ccatcttctg tttccggcat tcccggtata
     5041 ccaatgccga cagaacctct acagccaaaa cgttgatcag cttcggctac cagctcgcac
     5101 actgcatcta aaaatgcgtc atagctgtca cgcggtgtcg gcacccgctt ttcccactgc
     5161 aactgccgac cgctatcaaa cacgccaagc gcaatttttg ttccaccaat atcaaacccg
     5221 taatacattg ccgctccttg ttttaatgta ctgcctttac tggccgctaa ggactcgcgc
     5281 agggtcaata ttactggcgc gccgcgccgg ataccaactt gccaaaagac tcagcaacaa
     5341 tgctgtgacc agtacgtaga agacgtccag ccagtgcaat tccgatggca ggaagtcaat
     5401 aaaatagata tcgctggaga ggaactgatg accgatcagc ttttcaatcc gctcaataat
     5461 cggggtaagt tgcagtgaaa caactacgcc gataatcagc ccacacaggc tgccgaacag
     5521 ccctgccagc aatccatacc agacaaagat ggcgcgaatt aaaccatctt tcgcccccag
     5581 cgttcttaat actgcgatat cgccactctt atctttcacc gccatcacta aggtggagac
     5641 gatgttgaaa caggccacgc caatcaccag taccatcgcc agatacataa tggcgcggat
     5701 catctggata tcgcgataca tatagccgta agtaccaatc cagcttttaa tataaacata
     5761 gctgttggtc atttcacccg catcgcgtac cagcttattg gcgttgaaaa catccgtcat
     5821 tttaagggca atacctgaca cgctggaacc catatcaaga tattgttggg catccgccag
     5881 cgggatcatg gcaaaactgt gatcgagttg gccactcaac tgcaaaatac ccgcaacgtg
     5941 caaacgcaca cgttttggct gcatcagttt atgctcagga ttcgagttgg ggatcatgat
     6001 cgacacccaa tcgccctgct tcactttcag cgcatccgcc acgcctttgc cgatgataat
     6061 ttgctgttcg cccgctttaa aattgcgcca cgcatccccc tgaacaaacg agggtaatgc
     6121 gctcagacgc tgttcctgtt gtgggttaac gcccttcacc tggattgcgc gcagattcgc
     6181 tccactttcc accagcccgg tgaaattgat atacggcgca gcggcggcaa tacccggcac
     6241 tttctgcact ttatccagtg cttcctgcca gttagtccat ggctgattta ccgcttcgat
     6301 ttcaccatgc ggcaccactg ccagaatgcg gttattcagt tcgcgttcaa agccgttcat
     6361 cgcgcttaag ccgacgatca ataccgccac accgagggca atgccaatgg tagaaatgac
     6421 ggagatcagc gacaccatgc cgccgcgccg ccgtccgcga ctaaaacgca ggccaattaa
     6481 taacgataaa ggcatcgcca ttactccgcc cccatcaggc tcagttccgc cgtcagacgt
     6541 ccatcacgca tttctaactg gcggctcata cgtttcgcca gttgcaggtc gtgagtaacc
     6601 accaggaagg cggtgccctg caagcgattc aattccccaa gcaactggaa gatgctgtcg
     6661 gcgttacgcg catcgaggtt accggtaggc tcatccgcca gtaccagacg cggattattg
     6721 accagcgcac gggcaatcgc tacacgctgg cgttcgccgc cagaaagttc agatgggcgg
     6781 tgattcgcac gatgctccag ccccaccgct tttaacatct ccagtgcacg gctgttgatt
     6841 tcagcgggct ttttcttgcc aatcagcagc ggcatagcca cgttttccag ggcggtaaaa
     6901 tccggcagca ggtggtgaaa ctgataaata aagcccagtt tctggttgcg cagttctgct
     6961 tttgctgccg acgacagttt gctcatcggt tgaccgttaa agatcacatc gccggaggtt
     7021 ggcgtatcca gtccgcccag caggtgcagc aaggtgcttt taccggaacc agagctaccg
     7081 acgatcgcca tcatttcacc ttcgccgacg ctgaaactga cattgtgcag tacatcggtt
     7141 tgcacactgc cttcctgata gcgtttgcac aggttgtcgc attgcaacag gatcttattc
     7201 ataacgtaaa gcctcagcgg gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga
     7261 cagcagcgcg atagccatcg ccaccagcgc aataacaatg acctgtaaag gttcgatagc
     7321 catcggcagc gccgcgccat caagcaggac gccgattatt ggcatcagat tatttaactg
     7381 gctggcaagc agtgcgccaa gcgccgctcc gaggatcgca ccgataatcc cggcgctggc
     7441 cccttgcacc ataaagatca tcatgatttg tcgcggagtt aagccttgcg tttgcaggat
     7501 cgctacttcg ccctgcttct ccatcaccat cagccccagc gaggtaataa tgttaaacgc
     7561 cgcaacggcg acaatcaggc tcagcagtaa acccatcata tttttttcca tgcgtacggc
     7621 ctggaacaac tcgcctttac gatcacgcca gtcctgccat ttgctgtctt caggcagttt
     7681 ttgctgactt aatgagtcga ctttcagcgg ctcatccagc cacaaacgcc agccggtaat
     7741 attgcctgcc ggataacgca tcagacgcga ggcatcctca atattcacca gcatttcatt
     7801 gccatcgact tcactgttgg cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg
     7861 aatacgcccc atcggcgtga actggctggc agatggtacc atcatgcgga tttgatcacc
     7921 gcgattaacg cctagctgtg aggcaagttg ttcgccgagg atgacattat atttccccgg
     7981 ctcgaggtca gtttgtttca cattgaccag atacggcgtt agtggatctt tttgcgccgg
     8041 atcgataccc agcatcaccc cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc
     8101 ggtagtaata ggtgcgacgc gattaacgcc gtccagtttg actgccgttt ccgggagttg
     8161 ctgcgggtta agagagccat gctcagaaga gagaattgcc tgtggcatca ggccaaggat
     8221 gttgttttgc agctcgcgct caaagccgtt catcactgac aatactgtga ccagcgccat
     8281 caccccgagg gtaatgccga tggtagaaag ccaggagacg aaacgaccga agcgatccgc
     8341 tgcacgccca cgcatgtaac gcaggccaat aaatagagcg acaggttggt acatgaaatc
     8401 cgtctggttg ctgtagcaaa gacacgagta tataagcgaa agcaagatgg gtaaacgtct
     8461 gaactgtaag tgatcgtcag ggctaatctg aaaagccccc gtaaatcaat cggctaaatt
     8521 gccatgtttt atcatcaatc ttgccgcctg atcttaatct gaaaaatcat ccaatacaga
     8581 cagataccca ttacacctcg ggtcataacc cgcaggatca accgcatgta aattaaaaaa
     8641 gagcgtgttg tcctgatgaa acaaaaagag ctatggatta accagatcaa agggttatgt
     8701 atttgtccgg tggtgattta tcactcggtc attacctttt atccgcatat gaccactttt
     8761 ccgcatccgt tatcggaagt cctgagcaaa tgctggatct atttcaatct ttaccttgca
     8821 ccctttcgta tgccggtttt tttctttata tccggctatt tgattcgccg ctatatcgac
     8881 agcgtaccgt ggggaaattg tctcgataaa cggatctgga acatcttctg ggtgctggcg
     8941 ctttggggcg tggtgcagtg gctggcgtta agtgcgctga accagtggct ggcacctgag
     9001 cgcgatttaa gcaatgcctc caatgccgct tatgccgatt ccaccggtga gttcctgcac
     9061 gggatgatca ccgccagcac cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg
     9121 gtatgtaaaa tttttagccg tctggcgctg ccactattcg ccttgtttgt actgctgagt
     9181 gtggcggtta atttcgttcc cacgccgtgg tggggaatga acagtgtgat ccgcaatttg
     9241 ccttattaca gccttggcgc atggtttggc gcaacaataa tgacctgtgt taaagaggta
     9301 ccgttgcgcc gccatctgct gatggcttct ttgcttaccg ttctggcggt cggtgcctgg
     9361 ttgtttacta tctcgctgct gttgtcgctg gtatcgattg tggtgatcat gaagctgttt
     9421 tatcagtacg agcaacgatt cggtatgcgc tccaccagcc tgctgaatgt gattggctcc
     9481 aacaccattg ctatctacac cacccatcgc attctggttg aaatattcag cttaactctg
     9541 cttgcgcaaa tgaacgcagc acgctggtcg ccgcaagtcg aactgacact cctgctggtt
     9601 tatccctttg tcagtttgtt catctgtact gttgcgggct tgctggtaag aaaactttca
     9661 cagcgtgcat tcagcgatct gttgttctcc ccgccttcac tgcccgctgc cgtcagttac
     9721 tcccgctaag ccatcgcccc agccggggcg attcaatttg ctaaaccatg tcgattacgg
     9781 ataatgaaca gcattgctta tcagagatat gcccccatat gttgaggcat atcctaacga
     9841 gaatctgaca accgttatgc ctgaacaata tcgttatacg ctgcccgtca aagcgggtga
     9901 gcagcgtctg ctgggcgagt taaccggcgc agcctgtgca acgctggtag cggaaattgc
     9961 cgaacgtcac gccggtccgg tggtactcat tgcaccagat atgcaaaatg ctctgcgttt
    10021 gcatgatgaa atcagtcagt tcaccgatca gatggtgatg aatctggcgg actgggaaac
    10081 tcttccctac gacagctttt cgcctcatca ggacattatc tcctcgcgcc tttccaccct
    10141 ttaccagcta ccgacgatgc agcgtggcgt actgattgtt ccggtgaata cgcttatgca
    10201 gcgcgtttgc ccacacagtt ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg
    10261 cctgtcacga gatgcattac gaacccaact ggacagcgcc ggttatcgcc atgttgacca
    10321 ggtgatggag cacggcgaat acgccacgcg cggcgcgttg ctggatctct tcccgatggg
    10381 gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt
    10441 tgacgtcgac agccagcgca cgctggagga agtagaagcg atcaatctgc tgcccgcgca
    10501 cgaatttccg accgataaag cggcaattga actgttccgc agccagtggc gcgatacctt
    10561 cgaagtgaag cgcgatccgg aacatattta ccagcaagtg agtaaaggca cattacctgc
    10621 cgggatcgag tactggcagc cattgttctt cagcgaacca ctgccgccgc tgttcagtta
    10681 tttccctgcc aataccttgc tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt
    10741 ccaggctgac acgctggcgc gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt
    10801 gccaccacaa tcgctctggc tgcgggtgga cgagctcttc tcagagctga aaaactggcc
    10861 ccgggtgcag ctaaaaacta aacatttacc gacaaaagcc gcgaatgcca atttaggttt
    10921 ccagaaactg ccagacctag ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg
    10981 taagttcctc gagactttcg acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg
    11041 tgaagcgctg ggtgaactgc tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct
    11101 tgatgaagcc agcgaccgtg ggcgttatct gatgattggc gctgccgaac atggttttgt
    11161 cgataaggtg cgtaatctgg cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc
    11221 ccgccgtcgt caggattctc gccgcaccat caaccccgat acactgatcc gtaaccttgc
    11281 ggagctgcat attggtcagc cggtggtcca tctggagcac ggtgtcgggc gctatgccgg
    11341 aatgaccacg ctcgaagcgg gcggcattac tggcgagtat ttgatgctca cctatgccaa
    11401 cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg attagccgtt acgcaggtgg
    11461 cgcggaagaa aacgccccgc tgcataaact tggcggcgat gcgtggtcac gcgcgcggca
    11521 gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg ctggatatct acgcgcaacg
    11581 cgccgccaaa gagggcttcg cgtttaaaca cgatcgtgag cagtatcagt tgttctgcga
    11641 cagcttcccg tttgaaacca cgccggatca ggcgcaggcc attaatgcgg tacttagcga
    11701 catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac
    11761 agaagtggcg atgcgcgccg ctttcctggc agtagataac cacaagcagg tggcggtgtt
    11821 ggtgcctacc acccttctcg cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa
    11881 ctggccggta cgtatcgaaa tgatctcccg tttccgcagc gccaaagagc agacgcaaat
    11941 ccttgcggaa gtggcggaag ggaaaatcga tattctgatc ggtacgcaca aactgctgca
    12001 aagtgacgtc aagtttaaag atttaggcct gctgattgtc gatgaagaac accgcttcgg
    12061 ggtgcgtcat aaagagcgca ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac
    12121 tgcaacgccg atcccacgca cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat
    12181 tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc tttgtccgtg agtatgacag
    12241 cctggtggtc cgggaggcga tcctgcgtga aattttgcgc ggggggcagg tttattatct
    12301 ctacaatgat gtggaaaaca tccagaaagc tgccgaacgg ctggcagaac tggtgccaga
    12361 agcgcggatc gccatcggtc acgggcagat gcgcgagcgc gaactggaac gggtgatgaa
    12421 tgatttccat catcaacgtt tcaacgtgct ggtttgtaca accattatcg aaaccgggat
    12481 cgacatcccg acagccaaca ccattatcat tgaacgcgcg gatcacttcg gtctggcgca
    12541 gctgcaccag ttacgcggtc gcgtcggacg ttcacatcat caggcatatg catggctgct
    12601 gacgccgcat ccaaaagcga tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc
    12661 gctggaagat ctcggtgcag gctttgcgct ggcaacgcac gatctggaga tccgcggcgc
    12721 gggtgaactg cttggcgaag aacaaagtgg ctcaatggaa accatcggtt tctcgctgta
    12781 tatggagttg ctggaaaacg ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga
    12841 agatctcacc agccagcaaa cagaagtcga gctgcggatg ccgtcgctat tgccagatga
    12901 tttcattcct gacgtgaata cgcgtttgtc gttctataaa cgtattgcca gcgccaaaac
    12961 ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat cgcttcggcc tgctgccgga
    13021 tccggcgcgt accctgctgg atgttgcccg tctgcgccag caagcgcaga aactggggat
    13081 caggaagctg gaaggtaatg agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt
    13141 taatccggcc tggttgattg gtttgctgca aaaacagccg cagcattacc gccttgatgg
    13201 tccgacgcgc ctgaaattta ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt
    13261 acgccagttt atgcgtgaac tggaagagaa cgcgatcgct taatggtaaa tcgggggcgt
    13321 ttctgcgccc ccatcggcaa catttacaaa ttctttgcac ttccctgcac tatccgacac
    13381 tgtcaccatc cataattcag gcttatctgt ttattacaat aaccttatat ttattatgga
    13441 tttttggtga tgatcaaaac gcgtttttct cgctggctaa cgttttttac gttcgccgct
    13501 gccgtggcgc tggcgctacc ggcaaaagcc aacacctggc cgctgccgcc agcgggcagt
    13561 cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg acggtggttc tctggaagcc
    13621 atcgccaaaa aatataacgt cggctttctc gctctgttac aggctaaccc cggcgttgat
    13681 ccttacgtac cgcgcgcggg cagcgtgtta acgatcccgt tgcaaaccct acttccagac
    13741 gcgccgcgcg aaggcattgt gatcaacatt gcggagctgc gtctctatta ctacccgccg
    13801 ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc agttaggtgg tgacacgctg
    13861 acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa acccaacctg gacgccaacg
    13921 gcaaacaccc gcgcccgtta taaagcacag ggaattgagt tgcctgcggt agtgccggct
    13981 ggaccggata acccaatggg ccatcatgcg attcgtctgg cggcctatgg cggcgtttat
    14041 ttgcttcatg gtacgaacgc cgatttcggc attggcatgc gggtaagttc tggctgtatt
    14101 cgtctgcggg atgacgatat caaaacactc tttagccagg tcaccccagg caccaaagtg
    14161 aatatcatca acactccgat aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa
    14221 gtacatcagc cgctgtcaga gaagattgat gacgatccgc agctgctgcc aattacgctg
    14281 aatagcgcaa tgcaatcatt taaagatgca gcacaaactg acgctgaagt gatgcaacat
    14341 gtgatggatg tccgttccgg gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa
    14401 actctgtaaa tcctggataa aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc
    14461 agcagtagca ggcattaatg agggttaatg cttatttata aattactgct gttccatgga
    14521 gggtattcgg accggttaca gaagtaatac ggaaagattt tgcacccatc tcatccgctt
    14581 tttgcgccag ctgctcttcc agcgatccca gatttgtccc cgctttagca ctgattgtac
    14641 cgactttttg ttggccttct ggcgttgact gaacttcgac agccgcaaag ctggcaaatg
    14701 acatggagct taaaatcgcc gcagcgatga gggtttttac gtttttcata atagtggcct
    14761 tatgcagatg aatgacgtcg caagcaatta cttaacgatc gataactaaa tgacagatgt
    14821 gatctggatc acatacaaga tattttttat aacaatcatt aattaattta aaaatcttta
    14881 aatttcagtt aaatagaaag caattatttt tatgggatct ggactggtga acggaggtga
    14941 ttatttttat aataatcttt cattcacagt caccaggtac aacggcatca tggcaactga
    15001 ctcaacacaa tgtgtaaaaa aaagccgtgg ccgcccaaaa gtgttcgaca gggatgccgc
    15061 gcttgataag gccatgaaat tgttctggca acacggttat gaagcgactt ctcttgcgga
    15121 cctcgtcgaa gcgaccggag ccaaagcgcc cacgctatac gcggaattta ccaacaaaga
    15181 ggggttattt cgtgccgttc tcgaccgcta tatcgatcgt tttgccgcta agcatgaagc
    15241 acagctgttt tgtgaagaga aaagcgtgga gtctgcgctg gctgactatt ttgctgccat
    15301 cgccaactgc tttaccagca aagacacccc ggcaggctgc ttcatgatca acaactgcac
    15361 caccctctcc ccagattcag gagatatcgc caatacgttg aaatcacgcc atgcgatgca
    15421 agagcgcact ttgcagcagt ttttatgtca acgacaagcg cgcggggaaa tcccgaccca
    15481 ctgtgacgtg acacatctgg cagaattcct taattgtatt attcagggga tgtcgatcag
    15541 cgcacgcgaa ggtgcatcgc tggaaaaact aatgcagatt gccggaacga ctttgcgttt
    15601 atggcccgaa ctggtgaaat aagtcgcaaa aaaagcccct cagcctgagc gaatgagggg
    15661 ccaaattgca gagtacagcc ttgttatact ttgttattca aaatcagttg cccattacta
    15721 tctagcggga tctgcgtacc cggatcgcgg tccatgcgga ttttgccctg ctgatcgcca
    15781 atcttatagg tcacatcata accgagcatt ttttctgact tgtcatacac cgttttacaa
    15841 cgctgttgcg tagtcgtgta agtatcgctt tcctggagag agccctggat ctggttaccg
    15901 gcatatccac cacccagcgc ccccacaaca gtggcgacat ctttaccgcg accaccgcca
    15961 aactgatgcc ctatcacgcc gccagcaaca gcgccgagca ccgacccggt aatgcgattt
    16021 tcatcctgca ccggtcgacg atgggtcact gtgacgttgc gacactcctg acgcggtgtt
    16081 ttaaccgttt ccttgattgg ggttgcagaa acaacctgag cgtattgcgg gccacgttca
    16141 aacacgttca gactggccac tgccgctacg cccagcgcag ctgcgacacc aatcccgata
    16201 cccgccaaca ttgatttatt cacgggaaca cctccttctt tgcagttatg ccaatcatgc
    16261 aagcgaagga gaaaagcagc tatcagacag aagatgaaaa gcgcgggaat ggctggaaat
    16321 acttaatgat tcggaggagt ctgaaggaat gcctgatgcg acgcttaacg cgtcttatca
    16381 ggcctaccgc tcgtaccatt tgtccagggc ggatgaggcg tttatgccac atccgccagt
    16441 gtacgtcgat taatgcaact tcaaacgcgg acggataacg cggttaatac tccccaccag
    16501 catcattaat ccggttttaa agtaaccatg cagcgcaatc tgatgcattc ggtatagcga
    16561 gatatataca aagcgcgcaa ttcgtccttc aatcatcatt gagccgcgcg tcaagttacc
    16621 catcaggcta ccaacggtgg agaagttcga cagcgatacc agtgaaccat gatctttata
    16681 ctgataattt ttcagcggct taccgtttat ctgcgccaga atattgttca ttgcgcaagt
    16741 cgccatctgg tgtgcagcct gagcacgcgg tggaacaaag cccccttccg gacgcgtgca
    16801 tgacgcgcag tcgccaatag cgtaaatgtc tggatcgtgg gtggtttgca gcgtcggttc
    16861 caccaccagc tggttgatac ggttagtttc aagaccaccg atatctttca ggaagtctgg
    16921 cgctttgatc ccggctgccc acaccatcag atcagcctca atatattcac catctttagt
    16981 gtgcaggccg ccttcatcag cactggtgac catggtttgc gtcagcacgc gaacgccaag
    17041 tttcgttaac tcactatggg ccgcagcaga gatgcgtggc ggtaatgcag gcaaaatacg
    17101 ttctcccgct tctactagcg ttacgttcag ggcttcgttg gtcagacctt tataaccgta
    17161 gctgtgcagt tgcttgaccg cgttatgcaa ctcagcggag agttctacac ccgtcgcgcc
    17221 gccaccgaca atcgcaatgt tcactttgcc attcgcgccc aggttggcgg agtatttcag
    17281 gaacagattc agcatctcct ggtggaagcg acgcgcctgg tgcgggttat cgaggaaaat
    17341 gcagttctct ttgacgcctg gcgtattgaa atcgttagag gtgctaccca gcgccatgac
    17401 cagggtgtca taggcgattt tacgttccgg aaccagcagt tcacctttct cgtcgcgtag
    17461 ttctgcaata gtgattgttt tcgcttcacg atcaatatca atgacggaac ccaactggaa
    17521 ctggaaacca tgattgcgcg catgggccag atagctcaac gcatcgacgc cttcatcaag
    17581 cgagccagtc gccacttcgt gcagcagcgg tttccacagg tggctgtggt tacgatcgac
    17641 cagcgtaatt ttggcttttt tcttacgtcc cagcttatgc cccagctgtg ttgccatttc
    17701 cagcccacca gcaccgccgc cgacaatcac aatctttttc aatggcgtag tcaacgtgac
    17761 ccccttgaat ttattaacca attgttaatt aatggttatt aacatagctc ttaattaaca
    17821 acaggttaca gcaatgaaaa tgttcttcgc tattgagaat aacatgaatg gtgcattggt
    17881 cataccaaaa ttgatgttaa tcaagttttg ttgctgaaaa gataggcgac tttaccgggt
    17941 gagatcaaaa aatagagttt attgtttatt atttgagcga atttgtgaga gtgatataga
    18001 gtgaggggtg tatgcagggc gttcggtagg cctgataaga ggcgcatcag gcattgatgc
    18061 tcgactgtcg gatgcggcgt aaacgcctta tccgacttac agaattcgga gcgcatcagg
    18121 taccggcaag gttacctgat gcgacgaggg catttacccg agggttttga acgctttaat
    18181 gcgctgcaaa tgcggggaga tattcttgaa tttgtgcgtc tgctcttcgt cccagacaat
    18241 ctcgtaataa tgatgcaact cttcagatgt ccgctggctg ttaagcgctt catcattacg
    18301 cgacaaaatc accaggcaac gatcgcgatt cttctcgcgg aagttggtca cacacttagt
    18361 ggcaatatcg gcatactctt ccgggcgatc aatcttgcct tccatgttct cataagggaa
    18421 caaattaggg ttgaagatca cctggcggat atcgcaaaga aaaccaatcc gttccgccca
    18481 gtatccgccc agaccaacgc cgcaaatcag cggacgctcg tcgacgttca gttgcaacat
    18541 tttgtccact tctttaagca gatgctgcat atcatgtttc ggatgccgcg tactgtagct
    18601 tatcaagcgt acatccgggt caataaactg caattgtaag actttctcgt ggttacccgg
    18661 actgttagag tcaaaaccgt gtaaatagat aatcatcgct tcctcacata agccagggtt
    18721 agtgacctgc tttctcttcc tgccagcgtt catgtaactg attcagacgg gtgctgctcg
    18781 ccttccagcg agccgagtcc atcagttcct gtcgcgaaaa tgaaccttta tgatacaaac
    18841 gtgtaacacg ttctgccttg atcggtgaca gattatctaa cacgctgacg gcccctttac
    18901 gattattgca gaccaggatc atatcgcaac ctgcgtccag tgatgcctgc ccgcgttcgg
    18961 cataactgcc cataatcgcg gcaccttcca tcgataaatc gtcagagaaa attacaccgt
    19021 caaaacacag ttcctgacgc aaaacggttt tcagccagta agaagaaccg ctcgccggac
    19081 gcggatcaac atcactgtag atcacatgcg caggcataat ggcgtcgagt ttattttcgc
    19141 ggattaacgt gctgaaaacc gacatatctt tggcacgaat ttccgcttgc gggcgtgggt
    19201 cgcacggcgt ctctttgtgt gaatctgcag ttactgcacc gtgtcctggg aagtgtttcc
    19261 cggtcgtttt cattccggct tcatgcatcc catcaataaa ccgactagcg attgccaggg
    19321 ctttttctgg gtcggcatga taagaacgct cgccaatcgc cgcgctgata tgtcctacat
    19381 ccagcactgg cgcaaagctg atatcaatat ccatagcgat catttcgctg gccatcagcc
    19441 aacccgcctc ttgcgccagt ttgccgccct cttccattcc caacagcgca gcaaaggatt
    19501 gtgctgccgg taagcgggta aaaccttcgc gaaaacgctg cacgcgtcca ccttcctgat
    19561 ctaccgccac caccagatga ttgcgcgatg ctgcgcggat ctggcgcacc agttcacgta
    19621 actgggcagg atcatgatag ttacgcgtaa agagaatcag ccctcccacc agcggatgcg
    19681 ccagtatttc acgctcttcc gcgtccagtt cataaccttc gacatccaac attactggac
    19741 ccacactgct ctccttattg ttttattaat agctgccgcc aggtgtcatc ggccagcctg
    19801 ataaattgtt gatcgccggt ttgtcgccag cggtactcaa accaccctgc tttgagcatc
    19861 agcagccagg gaaaccatcg cctgacctga cgccataatt gcgccggata aatcttcgcg
    19921 cgagtggcat agtcattgac caattgccgg tgctgttcag tattttccac ccacaccgcc
    19981 gccagttcca gcgcggtatc accatctccg gcatactccc agtcgatgag ttttaaccct
    20041 gatgcgctat gcactaaatt tccggcgtgg acatccatat gcaatggact taagcgtaaa
    20101 ggtcgtggtt cccgcgcttt gcgcagacgt tttaacattc gcagccaacc cacagtccgc
    20161 cgcgccggat cgctttgctg ccagtacagt tccagtaacg gcaacagcgt tattcgccag
    20221 ccaaaacgtg gttgttgatg tagataatac agcaagcctg ccagttcgtt ggtatccggc
    20281 aaatacgttt ttaccgcgcc gggcagatag tcgactacca tccagtcacg gagatataaa
    20341 tgcggcttcg gtgcaatgct tgcgggtagt tgtgataaag cccgatactg gcgcaagaac
    20401 gcggactgcg gcgcatcagg atcgtgcggc tgacgcacaa caaaacgctg gccctgatgt
    20461 tcaatgagaa aactaccgcc actaagccca ctattaaacg tcgtgacggg atgaaactgc
    20521 gggaaaaagc gcgacagcaa ttcgtcgcgc gtgatgggat tattactgcg aaacggcacc
    20581 tttacctgac cagataattt cgcccgtctg caccagcatc agctgcattt gtagggtcgg
    20641 agcgttaacg ttgccagagg cgctggagta cagcacgtaa tgagcgccga cattgcgggc
    20701 aatgcctatg gctttactac gggtgcctaa actgtcctgc ggcgacaaac ctaactgttg
    20761 cttagccatc gacagttgct gggcggaaac cagggtaaat ttcccgttat tagccagcgc
    20821 atttcgcagc gtttcggtcg cttctgcggc attcagcgaa ccgttagtac ggttgttaac
    20881 gctatcaacc agcaggacgc tacccgcagt caccccgtca gccccaagca tcttactgac
    20941 tatcggctgc attgcgccat tccagtcata atggcgaata tgcggcgcag gcggtgccgt
    21001 tcgatcttcg tgctcaattg ggcctggctg ctgcgggatc gtcggcaccg agggcactgt
    21061 ggggacaggc tgttgtggct cggctggttg ttccggcgct ggtttcactt cttctaccgg
    21121 tgcaggttca cgttgcccca cacacccggc gagaaacatc gccagcgcgg taatcaaggc
    21181 gtagcgactc atttttgtca tcaagattca ccccttacaa atatagataa agtctgactt
    21241 tgtgcgcccc cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg
    21301 taacgctgcg tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa
    21361 aacgataatg aacggtaacg ggtttttgcc tttcgttata aagcgttgag gatgctgaag
    21421 gttgaatatc agacgtcgaa aggacgggct tttctgcact gatgcccgca gccaataaag
    21481 atgactccat caccagcgat tgctcatcat tcaccggaat ttccggatgt gaacgacagc
    21541 ccaccagcag taacaacacc agagacacca gcccaaagca tccttttctc atcgttacag
    21601 acctttatgc gccagcattg gtcccagcgg acggccaccc aacaagtgca tatggatgtg
    21661 gtaaacctct tgtccgccat ggcggttggt attcatgatc agacgatagc catcttcggc
    21721 aataccttct tgctcagcaa tttttgccac tacggtgatc atgcgtccca gcgcctgctc
    21781 atgctcagct gagacgtcgt tcacagtcgg aatgaggata ttcggaatga tcagaatatg
    21841 cgttggcgct tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct ggtagacgat
    21901 atcggagggg atctcacgac gaataatttt gctgaatata gtttcttctg ccacgacatt
    21961 ttcctttttc aaatagccat gcgttgcgcc atgctacgcc gcgccacact gcgagtatag
    22021 agtatggtcg agttaaacct gctctttcaa ccttaactca cgatttctta agcaaaaaat
    22081 cgttttgctg ccgtatatat cgccattatt cccatttctg cctgcgaatg cgctgaaaag
    22141 acattctctg tttggtttac acgacaaata tgaatgcgta tatttctcat ttgcatttac
    22201 aaacaaaatt attcgcacac taaatataat cgctcatcac cctcctccgg catgagcctg
    22261 acgacaacat aaaccatgag atttcaaatg ctttcaacac aatttaacag ggataatcaa
    22321 tatcaagcca tcaccaaacc gtcactactt gccggttgca tagcactggc actattacct
    22381 tctgccgctt ttgctgcatc agccactgaa gaaacggtga ttgttgaggg ttcagccaca
    22441 gctccagatg atggcgaa
//
LOCUS       DAJTSL010000058        22123 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTSL010000058 DAJTSL010000000
VERSION     DAJTSL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.058"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1298
                     /locus_tag="OO433_001978"
     CDS             <1..1298
                     /locus_tag="OO433_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6050471.1"
                     /translation="WLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPY
                     VRILTQDEIDVRIKRFWRYLDGTLPDAFMHANIGPSDSPITRAILRADAELKQVSPNL
                     TFIYDPEIPPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGG
                     GSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQIAIIDARCEFLYQQSHFFENSFLV
                     KEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVA
                     NTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPIIHLQTVAPHHAYYYSGI
                     SDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLSDLEKYR
                     AEGSRTNTTWLGEEATRNTRILERQPRVISHEQQMRFSQ"
     gene            1270..2133
                     /locus_tag="OO433_001979"
     CDS             1270..2133
                     /locus_tag="OO433_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6050472.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVGSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(2347..3129)
                     /locus_tag="OO433_001980"
     CDS             complement(2347..3129)
                     /locus_tag="OO433_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6050473.1"
                     /translation="MICRFIDTHCHFDFLPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(3126..4199)
                     /locus_tag="OO433_001981"
     CDS             complement(3126..4199)
                     /locus_tag="OO433_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6050474.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGCYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(4321..4482)
                     /locus_tag="OO433_001982"
     CDS             complement(4321..4482)
                     /locus_tag="OO433_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6050475.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="OO433_001983"
                     /pseudo
     CDS             complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="OO433_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
     gene            complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="OO433_001984"
     CDS             complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="OO433_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6050476.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(7287..7964)
                     /gene="yjjG"
                     /locus_tag="OO433_001985"
     CDS             complement(7287..7964)
                     /gene="yjjG"
                     /locus_tag="OO433_001985"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6050477.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     ELAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHLEQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="OO433_001986"
     CDS             complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="OO433_001986"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6050478.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKNFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRVLLKHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(8394..8807)
                     /gene="holD"
                     /locus_tag="OO433_001987"
     CDS             complement(8394..8807)
                     /gene="holD"
                     /locus_tag="OO433_001987"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6050479.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            8910..9941
                     /gene="rsmC"
                     /locus_tag="OO433_001988"
     CDS             8910..9941
                     /gene="rsmC"
                     /locus_tag="OO433_001988"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6050480.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            10209..10295
                     /locus_tag="OO433_001989"
     tRNA            10209..10295
                     /locus_tag="OO433_001989"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10243..10245,aa:Leu,seq:cag)
     gene            complement(10334..10570)
                     /locus_tag="OO433_001990"
     CDS             complement(10334..10570)
                     /locus_tag="OO433_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6050481.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            10711..11499
                     /gene="fhuF"
                     /locus_tag="OO433_001991"
     CDS             10711..11499
                     /gene="fhuF"
                     /locus_tag="OO433_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6050482.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(11537..12213)
                     /gene="bglJ"
                     /locus_tag="OO433_001992"
                     /pseudo
     CDS             complement(11537..12213)
                     /gene="bglJ"
                     /locus_tag="OO433_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(12171..12896)
                     /locus_tag="OO433_001993"
     CDS             complement(12171..12896)
                     /locus_tag="OO433_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6050483.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIVSLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            13515..14285
                     /locus_tag="OO433_001994"
     CDS             13515..14285
                     /locus_tag="OO433_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6050484.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAIITFFASTAAMYIRQLLAQ
                     HHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            14276..14749
                     /locus_tag="OO433_001995"
     CDS             14276..14749
                     /locus_tag="OO433_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6050485.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTRGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            14857..15396
                     /gene="dnaT"
                     /locus_tag="OO433_001996"
     CDS             14857..15396
                     /gene="dnaT"
                     /locus_tag="OO433_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6050486.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            15399..16136
                     /gene="dnaC"
                     /locus_tag="OO433_001997"
     CDS             15399..16136
                     /gene="dnaC"
                     /locus_tag="OO433_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6050487.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            16185..16679
                     /locus_tag="OO433_001998"
     CDS             16185..16679
                     /locus_tag="OO433_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6050488.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            16933..19224
                     /gene="opgB"
                     /locus_tag="OO433_001999"
     CDS             16933..19224
                     /gene="opgB"
                     /locus_tag="OO433_001999"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6050489.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRHRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(19363..20385)
                     /gene="lgoD"
                     /locus_tag="OO433_002000"
     CDS             complement(19363..20385)
                     /gene="lgoD"
                     /locus_tag="OO433_002000"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR6050490.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVRLGKNIADLKNGLQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            20524..21438
                     /locus_tag="OO433_002001"
     CDS             20524..21438
                     /locus_tag="OO433_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6050491.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTLINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            21653..>22123
                     /locus_tag="OO433_002002"
     CDS             21653..>22123
                     /locus_tag="OO433_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6050492.1"
                     /translation="MEKENITIDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVIND"
BASE COUNT         5469 a         5789 c         5460 g         5405 t
ORIGIN      
        1 aatggctgga gctggaaggc gcgaaagatc ttgatgacgc actctctctg ctgaccattc
       61 tttaccacca cgtaccgtcg gtcacatcga tgccggtcta cctagggcaa ctggatgcgt
      121 tgttgcaacc gtatgttaga attctaacac aagacgagat cgatgttcga ataaaacgtt
      181 tctggcgtta cctcgacgga accctgccag acgcctttat gcacgccaat atcggcccgt
      241 ctgattcgcc cattacccgc gcgatcttac gtgcagacgc agagttgaag caggtttcac
      301 cgaacctgac ctttatctac gatcctgaaa tcccccctga tgacctgctg ctggaagtgg
      361 cgaagaacat ctgtgaatgt agcaaaccgc acatcgccaa cggtccggtg catgataaaa
      421 ttttcacaaa agggggctac gggattgtga gctgttacaa ctcactgccg ctggcgggtg
      481 gtggcagcac gctggtacgc cttaacctga aagccattgc cgagcgcagc gaatcgctgg
      541 atgacttctt tacgcgcact ctaccgcact actgccagca gcagatcgcc atcatcgatg
      601 cgcggtgtga attcctctat caacaatcac acttctttga gaatagcttc ctggtgaaag
      661 aagggctgat taaccctgaa cgttttgtgc caatgtttgg catgtatggg ctggcggaag
      721 cggttaactt gctgtgtgag aaagaaggga ttgccgcgcg ctacggtaaa gaagccgccg
      781 caaatgaagt gggttatcgc atcagcgcgc aactggcgga gtttgtcgcc aatacccccg
      841 tgaaatatgg ctggcaaaaa cgcgccatgt tacacgcaca gtcggggatc agttccgata
      901 tcggcaccac gccgggcgcg cgtttgccgt atggcgatga gccagatccg atcatccatc
      961 tgcaaactgt cgccccgcat catgcttatt attattccgg catcagcgac attctgacgc
     1021 tcgacgaaac catcaaacgt aatccgcagg cgctggtaca gctttgcctc ggtgccttta
     1081 aagccggaat gcgtgaattt accgccaatg tcagcggtaa cgatctggtt cgcgttaccg
     1141 gttatatggt gcgtttgtcg gatttagaaa aatatcgcgc cgaaggttca cgcaccaaca
     1201 ccacctggct gggcgaagaa gccacacgca acactcgtat tctggaacgc cagccgcgcg
     1261 tgataagcca tgaacagcag atgcgcttta gtcagtaaga ttatcccctt ctcctgcgtt
     1321 gacgggccag gcagtcgtct ggctctgttt ctgcagggct gcaatctgcg ctgcaaaaac
     1381 tgtcacaatc cgtggacgat gggacgctgc aatgattgcg gagagtgcgt gccacagtgt
     1441 ccgcatcagg cgttgcagat tgttgacggc aaagtgctgt ggaacgctgt ggtctgcgag
     1501 cagtgtgata cctgcctgaa gatgtgtccg caacatgcca cgccaatggc gcaatccatg
     1561 agcgtggacg aagtgcttag ccatgtccgc aaagcggtgc tgtttattga agggataacg
     1621 gtaagcggcg gcgaagccac gacccaactg ccgtttgtag tggcgctgtt tactgctatc
     1681 aaaaacgatc cgcaactgcg ccacctcacc tgcctggtgg gcagtaacgg catgttgagc
     1741 gaaaccggct gggaaaaatt actcccggtg tgtgacggcg caatgctcga tctcaaagcg
     1801 tgggggagcg aatgtcatca acacttaacc ggacgcgata atcagcagat taagcgcagc
     1861 atctgtttgc ttgcagagcg cggcaagctg gcggaactgc gtttgctggt aattccagac
     1921 caggtggatt atttgcatca catcgatgag ctggcgacgt ttatcaagag acttggcgat
     1981 gttccggttc gcctgaatgc gtttcatgcc cacggcgtgt atggcgaggc gcaaagctgg
     2041 gcgagcgcca cgccggaaga cgttgagccg ttggctgatg cgttaaaggt gcgcggggtg
     2101 agccggttga tatttccggc gctctatttg tgacatgccg gatacggcct acggttcggc
     2161 acagacttgt aggcatgata agacgcgaca agcgtcgcat caggcattgt gcgccaactg
     2221 ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg cacaaacctg taggcatgat
     2281 aagacgcgac aagcgtcgca tcaggcatta tgcgccgact gccggatact gcctacgaag
     2341 ggtaaactac ccactaacgc taaacaacgc atacgtatta ttaagcaaca cttcggcaat
     2401 ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgca cagcctgttc
     2461 cggtcggtta ggctgcccct gaaagccgtt gagcggcata tccggcgcgt cggtttccag
     2521 caacaacgat gccagcggta attttgcgat gacatcgcgg gttttactgg cgcgtggata
     2581 ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct cggcctgttg
     2641 caggctgccg gaaaaaccgt gaaccacgcc agtgcgcgat aaatcgtggc gtttaagatg
     2701 catcgccagt ttgtcgtgtg tgcgccgtga atgcaggatc accggcagat cgtagcgttt
     2761 cgccagtttc agctgttcgt cgagtaacca ctgctgcctc tcaagttgcg gatcgtcgcc
     2821 gaagagatcc agaccgacct cccccactgc caccaccttc gccggacgcc tttccagcgc
     2881 ctgctgtagc tgctcaagag acacatcgct atgtttttcc aacatacctg gatgcaagcc
     2941 caatgcggca tacagcggtt gatatttttc cgctaacgcc tgcacacggg caaaattttc
     3001 cgcctctgtc gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc gttgcaggct
     3061 ggcctcttca tcgccactaa acggcaggaa atcaaaatgg cagtgggtgt cgataaaacg
     3121 gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcgtcaatc
     3181 acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaataacgat cgattccggc
     3241 gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag cttgccaacc
     3301 gtggcgagga aataacaacc gcataaacgc ccaagtttat agtcttcacg cagcgccgga
     3361 atccgactgc caagcgcgat actatgtaat ggcttcggcg gataaatttc gaatatccgc
     3421 agcttgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt ttcatgatgc
     3481 tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg
     3541 aaccactgcg gcgtgtagta catttgtgac ggcacagtgc gaatgacgac caacgtttta
     3601 gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc atccaggtag
     3661 ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga
     3721 atcacatcca gccagttttg tttggttggt aaaaagtaat taggcgcgta gtcatcctga
     3781 cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt
     3841 tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct
     3901 ccacggacaa agcgcaatgg gtcgaaaaat tcgcgttttg tggtatagcg catgatgact
     3961 ttacgcgcgt aaccgggctg attgcagata aacgccgaga ggttctgcgc cccggcagat
     4021 gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca
     4081 gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg tccaggctgg
     4141 aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccacc
     4201 tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa
     4261 aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat
     4321 ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc cgacgacaaa
     4381 gacaatttta gctgcgcctg cagcggtacc ggccaaacca ccaaacccaa gtgcggcggc
     4441 gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat
     4501 agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg
     4561 tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt acttagtttt
     4621 cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt cagcacggtc
     4681 actttgtgcc tgagaatcta cggtaccgga gagctgaacc acgccgtcgg tggtttcaac
     4741 tttcacatga cgggaaggga cgatatcgtc cgccagcagt ttggctttga tttcactggt
     4801 ggtggcggtg tcacccgcgt agcccttcac cgagccttct ttagcgtcgc gaacgtgcag
     4861 tttgtcgctg acagaggtca ccccttcaac gcctttcgcc actttcactg cctcttcggc
     4921 ctgagcctag ctttcaacga aaccgctcag ggtcacgact ttttgctcgg ttttgacaga
     4981 gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt tcgcggtgat
     5041 ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt tttgccctgc
     5101 gctttcattg gtagtctgtg cgttgttttc cgcgtaggca gagccggtcg cgacggcaga
     5161 ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag tcatcgattt
     5221 attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt gctgaatagg
     5281 gagagacttc accctctaca gaagtcaatg gccgccatca caaaagcgat gggtgatgaa
     5341 taacgaccat tacagcctct gaatcagtta ttaatatcgg tagaatgaca atcgacggct
     5401 ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg tcattacttt
     5461 gttaaatata gatcacaatt ttgaaaccgc tcgtgatatc agcgaaaaca taagcaaaag
     5521 tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga cagggcgcag
     5581 cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa ctgaacgtcg
     5641 ggataacgtt cctgcgccag gcgcaggttg accatgctgg tagcgatgta agcgaggtta
     5701 tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc gaatttcttc
     5761 gcgtccgcac attctaccca gcgggcagtg gcaacgttga ctgattcata tactgcttca
     5821 acgttgtatt cgctcttcag gcgcgatacc accacatcaa actgcagcac accaacagca
     5881 ccaacgatca aatcgttgtt ggagatcgga cggaacacct gcaccgcgcc ctcttcggaa
     5941 agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag gcggatacga
     6001 cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc accctgggta
     6061 aaggtgtcgc cgatctgaat agtgccgtgg ttgtgcaggc cgaggatatc gcccggatac
     6121 gcttcttcaa cgtgcgaacg gtcgcccgcc ataaaggtca gcgcgtcgga gataaccaca
     6181 tctttcgcag tacgcacctg gcgcagcttc atgccttttt catatttacc ggatactacg
     6241 cgcataaacg ccacgcggtc gcgatgtttc gggtccatat tggcctgaat tttaaatacg
     6301 aagccggtaa atttgtcttc gctcgcctct acggtacggg tatcagtctg acgcggcatc
     6361 ggcgcagggg cccactccac caggccatcc agcatatgat caacgccgaa gttacccagc
     6421 gcagtaccga agaataccgg agtgatttcg cccgcaagga acagttcttt gtcgaactcg
     6481 ttagacgcgc ctttcaccag ttccagttcg tcacgtagct gctgtgccag atcttcacca
     6541 accgcagcgt cgagatccgg gttattcagc cctttaacaa tacggacttc ctgaatcgtg
     6601 tggcctttac cgctctgata aagataggtt tcgtctttat aaaggtggta aacgccttta
     6661 aacagcttgc cgcagccaat cggccaggtg atcggtgcgc agccgatttt cagctcgttc
     6721 tcaacttcat cgagcagttc catcgggtcg cggatatcac ggtcaagttt gttcataaag
     6781 gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt acgatcttca
     6841 acaccttttg cagcgtcgat aaccatcagg cagcagtcca ccgctgtcag ggtacgatag
     6901 gtatcttccg agaagtcttc gtgccccggg gtgtctagca ggttaaccag gcaatcgtga
     6961 tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc catctccatc
     7021 cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccagc ggtctgaatg
     7081 gcctgtccga acagcagcac cttctcggtg atggtagttt taccggcgtc cgggtgagaa
     7141 ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa cgtcataatt
     7201 ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat
     7261 tttacccatc aacggggggg aggcaatcag tgtttacaca ggagctgctc cagttcgtgc
     7321 aacgatgaaa cggtccaggt gggcgcgatg ccttctggtt gctcgagatg gtgcgcattc
     7381 agccagcaag tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc ggcagtgtcg
     7441 ccaaccatca gcacgcgtga acggtcagga ttgcccgcca gttccagcgc ataatcgaaa
     7501 attttcttat tcggtttggc aatgccaact tcttcggaaa tcaccagcaa atcaaagtaa
     7561 tcacgcaggc ccgtccgttc cagacgcact tgctgcaagg cgctaaaacc gttggtgatg
     7621 atgccgattt tagcgttgcc acgaatggcg ttaagcagag aaaccgcgcc cggcagcggc
     7681 gtgcagattt ccgccatcgc attaataaag gcatcgttaa gcaagcctgc ttcaactttc
     7741 agccgttcgg cccagctctc gaaacgcccg tgctgaagct gtaatgaagt gatcgcgccg
     7801 ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa atcttcagcg
     7861 gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta
     7921 aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc accttgtctt
     7981 acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt
     8041 aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca atggcggcag
     8101 cgtttgaagc acggacttcc agccatagtg tcgccacgcc gcgtttttcc agttcgtcga
     8161 tcagatgttt cagcagcacc cttcccaatc cctgacgctg atagtcagga tcgaccacaa
     8221 tattgaacaa tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc gccattttgc
     8281 cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca aagttttttt
     8341 cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt aaatcagtcg
     8401 tttcgaggga agaaatcgtg ttcatatgtg caaatctgtt gccataacgc ggcgcgtgcc
     8461 gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc ttccagtgat
     8521 agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg
     8581 attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc gcgcagaaca
     8641 tcgctcacta aaggatcggt cagggcggga agatcgtttg ccaccatcac cagacggacg
     8701 tgtgccggga tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg cagcgaccac
     8761 tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc gaatcgctcc
     8821 tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata tggctataat
     8881 cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca agtgaagtct
     8941 tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga gacttacagg
     9001 atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag caattccacc
     9061 actggcaggt attaagccgc cagatggggg ataacgcccg tttcagtctg gtcgccacgg
     9121 tggatgacgt cgcagattgc gatacgctga tttactactg gccgaagaac aaaccggaag
     9181 cccagttcca gttgatgaat ttactttctc tgctgccagt ggggacagat atttttgtcg
     9241 ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggcagat tatgcgccgt
     9301 tgaataaagt cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc
     9361 cggtatttga tgccgataaa ttctggggcg aatacagcgt cgatggcctg acggtcaaaa
     9421 cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg ctgctctcga
     9481 cgttaactcc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg ggggtgcttt
     9541 cagttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgcgc
     9601 cagcggtaga agccagccgc gcaacacttg cggccaactg tgttgaaggt gaagtctttg
     9661 ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc aacccgccgt
     9721 tccacgatgg gatgcaaacc agcctggatg cggcgcaaac gctgattcgc ggcgcggtgc
     9781 gtcatcttaa tagtggcggc gagctgcgaa ttgtagcgaa cgccttcctg ccttatccgg
     9841 acgtgctgga tgaaacattt ggcttccatg aagtgattgc gcaaaccggg cgtttcaagg
     9901 tatatcgcgc cattatgacc cgccaggcga aaaaaggtta agtatcccgc cgcaagcctt
     9961 gctggatgcg gcgtaaacgc cttatccgac ctacaaaacc tgcacgttag cccttcgtag
    10021 gccagataag acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct accaaacctg
    10081 cacgctagtt tcctgatgga catttttcca gcaattacac ctccggcgat aattaactat
    10141 tgacgaaaag ctgaaaacca ctagaatgcg cctccgtggt agcaattctt tttaagaatt
    10201 gatggtatgc gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt agtgtcctta
    10261 cggacgtggg ggttcaagtc ccccccctcg caccaattat ctttacttcc tttcttgttt
    10321 cttccttgat ttttcatccc tgattcaaat tcattatcat cagcatgatg gcagcaataa
    10381 gcgccaggca cgatggtagc aaaacgtaat gccgtaactg tttgtgatac agcatcaccg
    10441 gcgtcaatac taatcccatc agaatgacaa tccgccactg atccggcgat aaatccgcat
    10501 acatcagccc tgcaatcagc aatcccggca gcaacactcc ccagccactg cccagcgtac
    10561 gttgcaacat ggtttcatct ctttcattga taatgataac taatatcata tgataatttt
    10621 tatcatttgc aagccagaga aatcccttgc tatcgggtaa acctatcgct aggattagca
    10681 atcattatca tttagattac tatcccgatt atggcctatc gttccgcacc gctctatgaa
    10741 gatatcatct ggcgaacgca tctccagccg caggatgctg ggcttgcaca agccgtacgt
    10801 gcgacgatcg ccaaacatcg tgaacatttg ctcgagttta tccgcctgga tgaacccgct
    10861 ccgcttaacg ccatgacgct ggcgcaatgg tcatcaccga atgcgctaag ttctctactc
    10921 gcggtctatt ccgatcatat ctatcgcaac caaccaacga tgatccgcga gaacaaaccg
    10981 ctgatctcac tatgggcaca atggtatatc ggcctgatgg tgccaccatt aatgctggcg
    11041 ctactgacgc aggaaaaggc attagatgtg tcgccggaac atttccatgc tgagtttcac
    11101 gaaaccggac gcgtcgcctg tttctgggtc gatgtgtgtg aagataaaaa cgcaacacca
    11161 cattcgccgc agcagcgaat ggaaacgtta atcagccagg cgctggttcc ggttgtgcag
    11221 gcactagaag cgaccggaga gatcaacggc aaacttatct ggagtaatac cggttatttg
    11281 atcaactggt atctcactga gatgaagcaa ctgctcggcg aggcgacggt tgaatcgctg
    11341 cgccatgccc tcttttttga gaaaacgctc acgaacggtg aagataatcc actctggcgt
    11401 accgtcgtgc tgcgcgatgg cctgctggtg cgccgaacct gctgccagcg ttatcgctta
    11461 ccggatgtgc agcaatgtgg cgattgtacg ctgaaatgaa agcactgccg gggaagtaaa
    11521 cccggcatca tgcggattaa tagggatgca acacattact tgcttcgcaa tgccgcatac
    11581 ataacagaat atctgcggcc tccaacaccc ctgcatcaga actgacgccc agtttcgaca
    11641 tcacattaaa tttatgtgca cggatcgttt tgatattgcg tttaagctgc tcggcaattt
    11701 gtgtcattga gtagccacgc gacataaagc gcaatatttc tctctccgtc gggcttagcg
    11761 tccggctttg gttaatgtac cactgattgt tcagtcggtc ggtcgcctga cgtacaccat
    11821 ttaatgacaa aaagagttcc tgatgaaaaa tctccagcgt tgacgcttta cttaatacac
    11881 cgtccagcgg tgatggcgac aatgaaccaa tcagccgagc ttcaatatca tcatccgcaa
    11941 taactaaacg ccgggtacgc ggaaacttaa tcgccagttc agtcaggcaa gataatccct
    12001 ctctgcgctc acttctcatg gcagaaaaag aaaaatgacc gccgcaaatg agatccgcga
    12061 catggcatcc tgaaatgact cctgactggt ataggtatgg agcttataag ggttacccgc
    12121 aaactttctg aataaactct caataccgat actgctcatg acgcattttt ctatgagtgc
    12181 gacatttctc ttcttaattc ggctgtgttc cattctgcgg catctccata aacagaaggc
    12241 aagttaagtt ctcttgcacc ctgcgcgcta tttatccatg catacatttc agcgttgctg
    12301 tgaattgcca gtcgacgcat cgcactgttt ttttgggcgc tgatagtttt attacttttc
    12361 ttaagcaacg aaactatctg gttgatgccc catcctttac ctaatagccg cagtaccttg
    12421 cgctctgaaa gagtgagaat gacggagcga tagctaaaat catgagtttc attcattgcc
    12481 ggagaggtca gcatggcgct gatacgctct gcgtgcgtat tgccggaacg tacggtcttg
    12541 accagattct caatgggttc aacatcagac aataacgtcg ccgtagggca catgagcaat
    12601 tctactgcct gggaatacca ggagcgtgac accatgaaaa cccagtgaat ttcccgatat
    12661 tgtgagatta aagaataata atgttcacaa acagaacgtg gatcttcact ttcaccggct
    12721 aaatcagcaa tgactaatcc ggaacgacgt aattgtaata aggtaaggtc ctccgcagag
    12781 gcgctggata ttatttcata ttcagggaag tgagtcctca tgacctcttt taaccctgct
    12841 tgcataacag gtattttact gataacaatt ccatttttgc agcatcctgg caacatattt
    12901 cctccacatc cactatctaa aaatcatgat ggtttattca ggatattcat tccttaaatg
    12961 tttccagcaa attaatagat taagcgaata aattatcctt agcattcact ttcatatcag
    13021 catactcctg ctgcacaaca actccactgc atgtgtcaga aggttagcat caaatgataa
    13081 cctagtgaaa tagcctggag acatttaaaa tatctacatt aagttgttag gaatacttaa
    13141 tgaaaaacag aaatcacatt catttaattt ataattaaat gatatccctt cccgcagaat
    13201 gaaaagtcac acaaccaata tttattcaat gaaatctgat ggttttaact tgttaacatt
    13261 aaaaaacgtg cccggttatc tattatcctg attcgtaaag gccaaacaat aaattcatta
    13321 tagtgataat attcatgtga tagtttgttt gcggcgagag ataattcgct ttttatcacc
    13381 gagcttgtcc cgataaccag gattaatcag cgcaattttg atcttaacct accgcaaaac
    13441 aaccgtgctt catgatacaa atggattctc attatctgaa caatacgcag cacgtctatg
    13501 acaaagggcg agttatgcaa actgagcaac agcgagccgt aacacggctt tgtatccagt
    13561 gtggattatt tcttttgcaa catggtgcgg aaagcgcgtt ggttgatgag ctttcctcac
    13621 gactgggtcg ggcgttgggg atggacagcg tcgaaagttc tatctcttcg aacgccatag
    13681 tgctgacaac cattaaagat gggcaatgcc tgacatcgac acgtaaaaat cacgatcgcg
    13741 gcattaatat gcatgtggtg actgaagtcc agcacattgt gattctggcg gagcaccatc
    13801 tgctggatta caaaggcgta gagaaacgat ttagccaaat tcagccatta cgttacccaa
    13861 gatggctggt agccttaatg gttggccttt cttgcgcctg tttctgcaaa ctcaataaag
    13921 gtggctggga tggtgccatc atcaccttct ttgccagtac ggccgcgatg tatatccgcc
    13981 agctgctggc acaacatcat cttcatccgc agatcaactt ttgcctcacc gctttcgccg
    14041 ccaccaccat ttccggattg cttttgcaac ttcccacttt cagcaataca cccactatcg
    14101 cgatggccgc cagcgttctg ctgctggtgc cgggatttcc gttgattaat gccgtcgccg
    14161 atatgtttaa aggccacatc aataccggac tggcacgctg ggcgatcgcc agtctgctga
    14221 cactggctac ctgcgtcggc gtggtgatgg cactgacgat ttgggggcta cgcggatggg
    14281 tgtgatcgag tttctgtttg cattggcgca ggatatgatc ctcgccgcca ttccagcggt
    14341 cggctttgcg atggtgttca acgttcccgt tcgggcatta cgctggtgtg cgctgcttgg
    14401 cgcgattggt catggttcac gaatgatctt aatgaccaga gggctgaata ttgagtggtc
    14461 aacctttatg gcctccatgc tggtcggcac cattggtatt caatggtcgc gctggtatct
    14521 ggcgcatccg aaagtgttta ccgtggcggc tgttatccct atgttcccgg gcatatcggc
    14581 ttataccgca atgatttcgg cggtaaaaat cagccagtta ggttacagcg aaccgttgat
    14641 gattaccctg ttaaccaact ttcttacagc ttcatcgatt gttggtgcgt tatccatcgg
    14701 tctttccatt cctggattat ggttgtaccg caagcgccct cgcgtataaa attgcctcgc
    14761 ttaaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct
    14821 cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg
    14881 gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaagctgaa
    14941 ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca
    15001 cgcctggctg aactgctggc gctggaagaa aagctggcgc gtccgggaag cgatgtcgct
    15061 ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc catggggaaa
    15121 ttcgccatgt atccggactg gcaacccgat gccgatttta tccgcctggc ggcgctatgg
    15181 ggtgtggcac taagagagcc ggtgaccacc gaagaactgg cctcattcat tgcctactgg
    15241 caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg
    15301 caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagcgaa
    15361 cctgacagtc aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga
    15421 tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa acgggtgaag
    15481 aactactggc ctggcagaaa gaacaaggag cgatccgctc cgccgctctc gaacgtgaaa
    15541 atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcggcca ctgcatcaga
    15601 actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag
    15661 cgcgccagta cgtcgaagag tttgacggca acatcgccag cttcatcttt tctggtaagc
    15721 caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta
    15781 aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat accttcagga
    15841 atagcggtac cagtgaagaa caactgctta acgatctgag caacgttgat ctgctggtga
    15901 tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg
    15961 tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag
    16021 agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttgt
    16081 gggtgatctt caactgggat agctaccgta gccgggtaac aggtaaagag tattaatccc
    16141 cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg aaaactgtta
    16201 aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg
    16261 cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagcacga
    16321 cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc gggaaccagg
    16381 ccttaagcgc agataacatg aacaacgccg caggcattct gcaatactgc gcgaagcaaa
    16441 agctggcgtc ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa aagctgggcc
    16501 tgaacagtga agagcaaaaa gaagacacca actatctgga tggcattcag ggtttgctga
    16561 aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg aacgactccg ctggcagaaa
    16621 aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc atttcctgaa
    16681 ccgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct gcccttttcc
    16741 ctgcttctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa aaaagtctgt
    16801 ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca gcaatatggc
    16861 gacgccatta cattgttctt ctcaaaaagc ttaaacgttg gctaaacggc ccggtttagt
    16921 gaggatcgcc cgttgtcaga actactctct ttcgcccttt ttctcgcctc tgtgctgatt
    16981 tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacattaac ggtgctgggg
    17041 ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg cgatggtatt
    17101 aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt cagcaaatac
    17161 atcctgccgg gtatcggcat tgtgctgggg ctgacagcgg tgttcggtgc gctgggctgg
    17221 atcctgcgcc atcgtcgcca tcatccgcac cattttggtt acagcctgct ggcgctctta
    17281 ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga actggtgaaa
    17341 tcccagtcac gcgatggcga cccggacttt gcggcttatt ataaagagcc gtcgaaaact
    17401 atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga gcggacctat
    17461 tttgataacg aagctttccc ggatctcacg cctgaactgg gcgcgttgaa aaatgaaggc
    17521 ctggatttca gccacacgca gcagctgcca ggaacggatt acacgattgc gggcatggtg
    17581 gcttctcagt gcggcatacc gctgtttgcc ccctttgaag gcaacgcctc cgcctctgtc
    17641 tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc gggttatcag
    17701 aactatttcg tgcagggcgc gaatctgcgt tttgccggta aagatgtgtt cctgaagtcg
    17761 catggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtggc cgacccgcac
    17821 tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg gaaaaagttt
    17881 gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt cgatacccat
    17941 cacccggatg gttttatctc gcgtacctgt aaccgcaaaa aatatgattt tgacggtaag
    18001 ccgaaccagt cattcagcgc ggtaagttgc agccaggaga atatcgcggc gtttatcaac
    18061 aaaatcaaag cgtcaccgtg gtttaaagat accgtcatcg tcgtctcttc tgaccattta
    18121 gcgatgaaca acacggcgtg gaaatacctc aataagcagg atcgcaataa cctgtttttt
    18181 gtcattcgtg gcgacaagcc gcagcaagag acgctggcag tgaagcgtaa cacgatggat
    18241 aacggcgcga cggtgctgga cattctcggt ggcgataact atctcggact tggtcgtagc
    18301 agtttatccg ggcagtcgat gtcggaaatc ttcctcaata tcaaagagaa aacattggcg
    18361 tggaagccgg atatcatccg cctgtggaaa ttccctaaag agatgaaaga gttcaccatc
    18421 gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc gctgctgttg
    18481 cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg aatactcagc accgctgcgt
    18541 ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgaccg ttgctataag
    18601 atggcacaac tctgggctcc ggaactggca ctctccaccg actggtgtgt ctcgcaaggg
    18661 cagcttggcg gtcagcaaat tgttcaacat gttgacaaaa caacatggca gggcaaaacg
    18721 gcatttaaag atacggtcat cgacatggcg cgttataaag gcaatgtcga tacgctgaaa
    18781 attgttgata acgatattcg ctacaaagcc gacagtttca tcttcaacgt cgccggtgcg
    18841 ccagaagagg tgaaacagtt tagtgggatt tcccgtccag agtcgtgggg ccgctggtcc
    18901 aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc aaagaaattt
    18961 gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat tccggtacgc
    19021 gtaggcaatg aagaacaaac ccttgtgctg ggcaatgaag tgaccaccac cacactgcat
    19081 ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc tgtctcaacc
    19141 aacgaaggga atatcctcgg acactcgcca cgtaagctcg ggatcggcat ggtggaaatt
    19201 aaagtggtgg aacgtgaagg ataattcctg actgatgtgt aggtcgcata aggtgcgacc
    19261 tacacacctt ctgcggccta caaaacatca ttgagaaaca gaaaaaaaca gcgcccaaca
    19321 cttccccgga cgctgcatgg caactttaat aatctcgcct tatcagaaag taattacacc
    19381 cttaatcaac tcacgattgt taatcacatc gcgctcgtag gtttctgcca gcgtggcgaa
    19441 cggatagcga tgggttaaca tcatgtcagc agtgattttt ccttccgcca tcagtcgacc
    19501 gactttagca aaatcttccg gcgtggcgtt gcggctaccc atcatcgtcg tttctttttt
    19561 atggaattcc ggatcggaga actgcaactc acctttaaac aggccgacaa ataccaccgt
    19621 gccgccgtga cgaatcaaat tcacggtgtt attcatcgca tgttgattac ctgtcgcgtc
    19681 gatcactttc tgcgccagcg aaccaccaaa ctgcgcccgt agctgcgcgt caaaatcttc
    19741 ggctgacggg tccagtaaag gtaattccag acgcgttgcc acatgttcac ggcgcgccgg
    19801 actggtatcc gccaccacca cctgtgcgcc atcggcttta gcgattgccg ccgcgcccag
    19861 accgattggc cccgccccga ccaccagcac ctgctcgccg ggagcaatgg ctgcgcgacg
    19921 caccgcatga gcgctaatag cgaaaggttc aatcaatgct gccgcctgcg ggtcaatacc
    19981 gtctgcgggc aaaatgttcg ccaccggcac gctcaaatac tcactaaaac cgccatcctg
    20041 atgcacgcca atgactgaaa ttttttcaca gcaattggta cgcccgcttt tacacgccgg
    20101 gcattgctga caggcaacat aagggatcac agcaacttgc agaccatttt taagatcagc
    20161 aatattttta cccagcctaa caatctcccc acatatttca tggcctaaaa cacgtggata
    20221 actaaaaaat ggttgatttc caccccaggc atgaatatcg gtaccgcaaa tcccgacaga
    20281 cttaattttt attaatgctt cattgtcacc cggaataggt atctcgcgtt gtttccagac
    20341 taattctttc ggctgctggc aaattaaaac attcatcgta gacatgattc ggctctccat
    20401 tttttgttgc cttagatatc aatgaaaatg aaaaatggca gcggaacgga aaatctgttt
    20461 tgtgaaaaca cacgcataaa aatgttttaa atcgggtttt aatggactca caaggaggaa
    20521 aatatgagtc gttcacaaaa tttacgccac aatgtgatta accaggtcat tgatgatatg
    20581 gcgcggggtc atatcccttc cccacttcca tcgcaaagtg cgctggcgga aatgtacaac
    20641 atcagccgca ccacagtgcg tcatattctc agccatttac gcgaatgcgg cgtcttgacg
    20701 caggttggaa acgactatgt tattgcgcgt aaacctgacc acgatgacgg tttcgcctgt
    20761 actaccgctt caatgagtga acaaaacaaa gtgtttgagc aggctttttt cacgctgata
    20821 aaccagcgcc agttacgccc aggggaaact ttttctgaac tgcaactggc tcgagccgcg
    20881 ggtgtcagcc cggtggttgt aagagaatat cttttaaaat tcggccgtta taatcttatt
    20941 caaagcgaaa agcgcggcca gtggagcatg aagcaattcg atcaatccta cgccgagcag
    21001 ctgtttgagc tacgcgaaat gctcgaaaca cattcacttc agcactttct caacctgcct
    21061 gatcatgatc cccgctggct gcaggcgaaa accatgctcg aacgtcatcg gctactgcgt
    21121 gacaacatcg gcaatagctt tcgcatgttc tcgcaacttg acagagattt tcactcctta
    21181 ctgctttcag ctgccgacaa tatctttttt gatcaatcgc ttgagatcat ctcagtcatc
    21241 tttcattttc actatcaatg ggacgaaagc gatcttaaac agcgcaatat cattgccgtc
    21301 gatgagcata tgaccattct cagcgctctg atttgccgca gtgacctcga tgccaccctt
    21361 gcgctccgta accatttgaa ttcagctaaa caatcaatga ttcgatccat taatgagaac
    21421 acgcgttatg ctcattaagt acggattaaa aacagcaaac tattagcggg tgcacggttt
    21481 tatcaaaaga taaataccgg gaaattcccg cttacctatg ctcacaatca gtcgactaaa
    21541 aatgtttttg tgagcggtag taaagtccta aaactttaac ctgccgttaa tgtcaacctt
    21601 gcgtaatgca caattacgta aacaattaaa attataaata caggagtccg gcgtggaaaa
    21661 agaaaatatc accatcgatc cgcgttcttc atttactcca tcttcgtcgg cagatattcc
    21721 cgtgccacca gatggattag ttcaacgcag tacccgaatt aaacgcattc aaaccaccgc
    21781 catgttgtta ttattttttg cggcggtaat caattatctc gaccgcagtt cgctgtcggt
    21841 agcaaattta acgattcgtg aagaattggg attaagtgcc actgaaatcg gcgctttgct
    21901 ctccgtgttt tcactcgctt acgggattgc gcaacttcct tgcggcccac tattggatcg
    21961 taaaggccca cgcctgatgc tgggactggg gatgttcttc tggtcactgt tccaggcaat
    22021 gtctggcatg gtgcacaact ttacgcagtt cgtgttggtg cgtatcggta tggggattgg
    22081 tgaagcgccg atgaacccat gcggtgtaaa agtcattaac gac
//
LOCUS       DAJTSL010000059        21723 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTSL010000059 DAJTSL010000000
VERSION     DAJTSL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.059"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..421
                     /locus_tag="OO433_002003"
     CDS             <1..421
                     /locus_tag="OO433_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6050493.1"
                     /translation="SSKRSNVAQMPRSPLTKERQRRANAQVAANKRAATAAANKKARQ
                     DKLDAEASADKKRDQSYEDELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQT
                     DVVQSEADANRNMTEGGRDLMKSVGKAEENKSDSWFN"
     gene            complement(516..2093)
                     /gene="guaA"
                     /locus_tag="OO433_002004"
     CDS             complement(516..2093)
                     /gene="guaA"
                     /locus_tag="OO433_002004"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6050494.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMHMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYNKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2162..3628)
                     /gene="guaB"
                     /locus_tag="OO433_002005"
     CDS             complement(2162..3628)
                     /gene="guaB"
                     /locus_tag="OO433_002005"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6050495.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            3790..5160
                     /gene="xseA"
                     /locus_tag="OO433_002006"
     CDS             3790..5160
                     /gene="xseA"
                     /locus_tag="OO433_002006"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6050496.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5157..5372)
                     /locus_tag="OO433_002007"
     CDS             complement(5157..5372)
                     /locus_tag="OO433_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6050497.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5441..6913)
                     /gene="der"
                     /locus_tag="OO433_002008"
     CDS             complement(5441..6913)
                     /gene="der"
                     /locus_tag="OO433_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6050498.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEVEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7031..8209)
                     /gene="bamB"
                     /locus_tag="OO433_002009"
     CDS             complement(7031..8209)
                     /gene="bamB"
                     /locus_tag="OO433_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6050499.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYD
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8220..8840)
                     /gene="yfgM"
                     /locus_tag="OO433_002010"
     CDS             complement(8220..8840)
                     /gene="yfgM"
                     /locus_tag="OO433_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6050500.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(8858..10132)
                     /gene="hisS"
                     /locus_tag="OO433_002011"
     CDS             complement(8858..10132)
                     /gene="hisS"
                     /locus_tag="OO433_002011"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6050501.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDLNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPIVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     TNGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10243..11361)
                     /gene="ispG"
                     /locus_tag="OO433_002012"
                     /gene_synonym="gcpE"
     CDS             complement(10243..11361)
                     /gene="ispG"
                     /locus_tag="OO433_002012"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6050502.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11388..12401)
                     /gene="rodZ"
                     /locus_tag="OO433_002013"
     CDS             complement(11388..12401)
                     /gene="rodZ"
                     /locus_tag="OO433_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6050503.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLLGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(12686..13840)
                     /gene="trmG/rlmN"
                     /locus_tag="OO433_002014"
     CDS             complement(12686..13840)
                     /gene="trmG/rlmN"
                     /locus_tag="OO433_002014"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6050504.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(13990..14421)
                     /gene="ndk"
                     /locus_tag="OO433_002015"
     CDS             complement(13990..14421)
                     /gene="ndk"
                     /locus_tag="OO433_002015"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6050505.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(14570..16882)
                     /gene="pbpC"
                     /locus_tag="OO433_002016"
     CDS             complement(14570..16882)
                     /gene="pbpC"
                     /locus_tag="OO433_002016"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6050506.1"
                     /translation="MLRLLTKRGCWITLAAVPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLEWPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHYAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(16883..>21723)
                     /gene="yfhM"
                     /locus_tag="OO433_002017"
     CDS             complement(16883..>21723)
                     /gene="yfhM"
                     /locus_tag="OO433_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCR6050507.1"
                     /translation="SENASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVL
                     TFSIPLDPDQDFSRVIHVVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEV
                     KALNNATFSKDYEKTITTRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFR
                     VKPESLPAFISQWEYRNSLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDI
                     KPLQQAGVYLAVMNQAGRYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQ
                     GIEVSLLNEKGQTLTQATSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAE
                     FNIAGAPGYSKQFFMFGPRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQV
                     LRSVVSQPENGLYHFTWPLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNL
                     TGEKTPLTPNDEVKFSVVGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAA
                     ENLSRTLDEVQLTLDDKGRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQA
                     IWPANALPGLRPQFALKSVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGL
                     QVRLIRERRDYYWNWSEDEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRL
                     EVKAPNEAVSSVRFWAGYSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPT
                     AGKGYAMVESSEGPLWWQEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPSDKSRSA
                     TPKRAVGVLHLPLGDENRRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVS
                     AVDSGVLNITDYVTPDPWQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELK
                     RGGKPLVNHVNIVAQQALPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNES
                     KVIVAAPVIAELNMPRFMASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPA
                     AVELAPGVRTTLFIPVRALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPA
                     QTVNYGTALQPGETWAIPADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGC
                     LEQTASGLFPSLYTNAAQLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDK
                     NGDEEYWLTAYVMDFLVRAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKA
                     SKFAVQSYAALVLARQQKAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEE
                     AIVLALKTPRNSDERIWLGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFG
                     ERWLSTQESNALFLAARTIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVR
                     NSGDQPLWLRMDASGYPQSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQ
                     VKASNSVPDALVVDLLPAGLELENQNLANGSASLEQSGDEVQNLLNQMQQASIKHIEF
                     RDDRFVAAVAVDEYQPVTLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIV
                     RP"
BASE COUNT         5154 a         6114 c         5391 g         5064 t
ORIGIN      
        1 atccagcaag agaagcaacg tcgcgcaaat gcccaggtcg ccgctaacaa aagagcggca
       61 acgtcgcgca aatgcccagg tcgccgctaa caaaagagcg gcaacggcag cagcaaataa
      121 gaaagctcgt caggataaac tggacgccga agcctctgcg gacaaaaaac gcgatcaaag
      181 ttatgaagat gagctacgca gcttagagat tcagaaacaa aaactggcgc tggcaaaaga
      241 agaagcccgc gttaagcgag aaaacgaatt tatcgatcag gaactgaagc acaaagctgc
      301 gcaaaccgat gtggtgcaat ctgaagctga cgccaacaga aatatgactg aaggcggtcg
      361 cgatctgatg aaaagcgtgg gcaaagcaga agagaacaaa tcggacagct ggtttaatta
      421 atcgatgtta gtaacttcaa gcctatgatt cttgaagata aaaaaccctc tgtagtaaca
      481 gagggttttg ttcattcata gtgcagggat taaaatcatt cccactcaat ggtagctggc
      541 ggcttgccgc tgatgtcata caccacgcgg gaaataccgt tcacttcatt gataatgcgg
      601 ttggaaacgc gaccgaggaa atcgtacggc agatgcgccc agtgtgcggt cataaagtcg
      661 atggtttcga cagcacgcag agagacaacc cagtcatact tacgaccatc gcccattacg
      721 ccaacggaac gtaccggcag gaacacagtg aacgcctggc tgactttgtt gtacaggtcc
      781 gctttacgca gttcttcaat gaagatggcg tcagcacggc gcagcaggtc acagtactct
      841 ttcttcactt cacccagcac acgaacgcca aggcctggtc ccgggaacgg gtgacggtac
      901 agcatgtcgt acggcaggcc cagctccaga ccaatcttac gcacttcgtc tttgaacagc
      961 tctttcagcg gttcaaccag gcccatcttc atctctttcg gcaggccgcc cacgttgtgg
     1021 tgagatttga tgacgtgtgc tttaccggtt gcagaagccg cagattcgat aacgtcaggg
     1081 tagatggtgc cctgcgccag ccacttcacg tcttccagtt tcagcgcttc ttcatcgaac
     1141 acttcaacga aaacgcgccc gatgatttta cgttttgctt ccggatcgtt ttcgccagcc
     1201 agcgctgaca ggaagcgatc ttctgccggt acgtgaacaa tgttcagacc aaagtgatcg
     1261 ccaaacatat ccagaacctg ctctgcttcg ttgagacgca gcaggccgtt gtcgacgaat
     1321 acgcaagtca gatttttacc gatagcgcgg tgcagcagca ttgcggttac ggaggaatcc
     1381 acaccaccag agaggccgag gatgacttta tcgtcgccta cctgctcgcg gatgcgagct
     1441 acagcatcgt cgataatttt cgctggcgtc cacagggctt cacactggca gatatcacgc
     1501 acaaaacgct ccagcatgtg cataccctgg cgggtgtgag tcacttccgg gtggaactgt
     1561 acgccataga agcgtttttc ttcgttagcc ataatggcaa acgggcagct ttcggtgctg
     1621 gctacggtga cgaagtcgga cgggatagcg gtaactttat cgccgtggct catccagaca
     1681 tcgagcagcg gtttaccgtc tgcggtcagc gcatcttcga taccgcgaac cagtgcgctg
     1741 tcgtttacga cttcaacctg cgcgtagcca aattcacgtt cgttagaggc ttcaacgtga
     1801 ccgcccaact gcattgccat ggtctgcatg ccatagcaaa cgccgaatac cggtacgcct
     1861 gcttcaaaga catactgcgg cgcacgcgga ctgttttctt cagtagtact ttccgggccg
     1921 ccggaaagaa taatgccgct tggattgaag tcacgaattt gtgcttctgt cacatcccac
     1981 gcccacagtt cgcagtaaac acccagctca cgcacgcggc gcgcaaccag ttgagtgtac
     2041 tgagaaccga agtccagaat gaggatgcga tgcttatgaa tgttttccgt cattgacgct
     2101 tattccgagg caagtgaaac agataatata aatcgcccga catgaagtcg ggcgaagaga
     2161 atcaggagcc cagacggtag ttcggggact ctttagtaat ggtcacgtcg tgaacgtggc
     2221 tttcctgaat gcccgcaccg ctgatacgta caaactccgc tttagtacgc agttcgtcga
     2281 tagtaccaca gccggtcagg cccatacagg agcgcaggcc gcccatctgc tggtgaatga
     2341 tctctttcag gcgaccttta taggctacgc gaccttcgat accttccggc accagtttgt
     2401 cggcagcgtt gtcactctgg aagtaacggt cagaagaacc tttggacatc gcacccagag
     2461 agcccatacc acggtaagat ttgtaagaac ggccctggta gagttcaatt tcacccggag
     2521 attcttcagt acccgccagc atggaaccca ccattaccgc gcttgcgcca gcggcgatag
     2581 ctttggcgat gtcgccggag aagcgaatac caccatctgc gataaccgga atacctgtgc
     2641 cttccagcgc ttcaactgcg tcagcaacgg cagtgatctg cggaacacct acgccagtaa
     2701 cgatacgagt cgtacagata gaaccagggc cgataccgac tttaaccgcg ctgcaaccag
     2761 cttctgccaa agcgcgtgca cctgcagctg ttgccacgtt gccgccgatg atttgcagat
     2821 ccggatattt agcacgggtt tcacggatac gctgcaaaac gccttctgag tggccgtggg
     2881 aggagtcgat cagcagaacg tcaacgccag cggcaaccag cgcgtcaaca cgctcttcgt
     2941 tacccgcacc tgcgccaact gcagcaccaa cacgcagacg gccttgctcg tctttacagg
     3001 cgttcggttt acgttccgct ttctggaagt ctttcacggt gatcatgccg atcaggtgga
     3061 attcgtcatc aaccaccagc gctttttcaa cgcgtttttc gtgcattttt gccagcacca
     3121 cttcacgggc ttcaccttca cgcacggtga ccagacgctc tttcggcgtc atgtaaacgc
     3181 taaccggctg gttcaggtcg gtaacaaaac gcacgtcacg accggtgata atacccacca
     3241 gttcgttttc ttcggtaaca accggatagc ccgcaaaacc gttacgctcg gtcagttctt
     3301 tcacttcgcg cagcgtcgtg gttggcagaa cagtctgcgg atcagtcacc acaccagatt
     3361 cgtgtttttt cacacggcga acttcttctg cctggcgttc aatggacatg tttttgtgga
     3421 taaagccgat accgccttcc tgagccagag caatagccag gcgcgcttcc gttacggtat
     3481 ccattgctgc ggaaagcata gggatattca gacgaatagt tttcgtcagc tgggtgctga
     3541 ggtcagcagt attcggcaga acggtagaat gagcaggaac gaggagaacg tcgtcaaacg
     3601 tcagagcttc tttagcgata cgtagcatgg gcaatatctc gaccagagtg gttaataaat
     3661 attgccgcgg cattatacag agcgtaaccg attgcatcta cccctttttg caaaaaatgc
     3721 ttgctatccc cgaagggcgg gttactatcg actgaataac ctgctgattt agaatttgat
     3781 ctcgctcaca tgttaccttc tcaatcccct gcaattttta ccgttagtcg cctgaatcaa
     3841 acggttcgtc tgctgcttga gcatgagatg ggacaggttt ggatcagcgg cgaaatctct
     3901 aatttcacac aaccggcttc cggtcactgg tactttacac tcaaagacga caccgcccag
     3961 gtacgctgcg cgatgttccg caacagcaac cgccgggtga ccttccgtcc acagcatgga
     4021 caacaagttt tagttcgcgc caatattacg ctctacgagc cgcgcggtga ctaccagata
     4081 atcgttgaga gtatgcagcc ggccggtgaa gggctgctgc aacagaagta cgaacagctc
     4141 aaagcgaagt tgcaggctga aggtttgttc gatcagcaat acaaaaaacc acttccctcc
     4201 cctgcgcatt gcgttggtgt gatcacctca aaaaccggtg ctgcgctaca tgatattttg
     4261 catgtgttaa aacgtcgcga tccttctcta ccggtgatca tctaccccac cgccgttcag
     4321 ggcgatgacg caccggggca aattgttcgc gccattgaac tggcgaatca gcgcaatgag
     4381 tgcgacgtgt tgatcgttgg gcgcggcggc ggttcgctgg aagatttatg gagttttaac
     4441 gacgaacgcg tagcgcgggc gatttttgcc agccgcattc cggtcgtcag cgccgtcggg
     4501 catgagacgg atgtgaccat tgccgatttt gttgccgatc tgcgtgcgcc aacgccgtct
     4561 gccgccgctg aagtagtgag ccgtaatcag caagagttac tgcgccaggt gcaatcgacc
     4621 cgtcaacggc tggagatggc gatggattat tatctcgcca accgcacgcg tcgctttacg
     4681 cagatccatc accgattaca gcaacagcat ccgcagctcc ggctggcacg ccagcaaacc
     4741 atgcttgagc gcctgcaaaa gcggatgagc tttgcgctgg aaaatcaact taagcgtacc
     4801 gggcaacagc agcagcggtt aacacagcgg ctgaatcagc aaaatccaca gccgaagatt
     4861 catcgcgcgc aaacgcgcat tcagcaactg gaatatcgtt tagcagaaac cctgcgcgta
     4921 cagcttagcg ccacgcgtga acgtttcggt aatgcagtaa cgcacctcga agccgtaagc
     4981 ccactgtcaa cgctggcgcg tggatacagc gttactactg ctactgacgg caatgtactg
     5041 aaaaaagtga agcaagttaa agcgggtgaa atgctaacca cacgtctgga agacggctgg
     5101 atagaaagtg aagttaaaaa catccagcca gtaaaaaaat cgcgtaaaaa ggtgcattaa
     5161 gccagcacga attcaacgcg ttttttcgat atcaagccat gcccgtgctg acagaagtaa
     5221 tctactgcac cgcaggcttt taacacctgc aacggttgat ggcagtctgg acaaagagct
     5281 ttcatttcaa taatttcccc gcaggatggg caacgcgcat ggccgttatc ctgatcaagc
     5341 acatgctgac attgtggaca atgcagttcc atatgaattc ctccggtagc catccctcta
     5401 cattcataga gggaatggca gataaaatac ttacggataa ttatttattt ttcttgatgt
     5461 gcttcatcag acgcttacgt ttacgcatct gggttggcgt cagggtgtta cgcttgttcg
     5521 catacgggtt ttccccttct ttgaactgaa tacgaatcgg cgatcccatt acgtccagcg
     5581 atttgcggaa gtagttcatc aagtagcgct tgtaggaatc aggcaggtct ttcacctgat
     5641 taccgtgaat caccacaatc ggcgggttat aaccaccagc gtgggcatat ttcagcttca
     5701 cacgacgacc gcgtaccagc ggcggttggt gatcttcaac agccatcgtc atgatgcgcg
     5761 tcagcataga ggtccccaca cgacgggtgg agctgtcata cgcttcacgt actgattcaa
     5821 acaagttacc aacaccactg ccgtgcaagg cagagataaa gtgcacacga gcaaaatcga
     5881 taaagcccag acggaagtcc agcgtttctt tcacctgctc tttcacttcc tggctcaggc
     5941 catcccactt attcaccaca atgacaagtg agcgcccact attgagaata aagcccagca
     6001 gcgagagatc ctgatcggaa ataccttcgc gcgcatcaat cactaacatc accacgttgg
     6061 catcttcaat ggcctgcaac gttttgatta cggagaattt ctctacagca tcggtgattt
     6121 tgccgcgttt acgtacgcca gcggtgtcaa tgagcacata ctcacgtcca tcgcgttcca
     6181 ttggaatgta gatactgtca cgcgtcgtgc caggcatgtc ataaacaaca acgcgctctt
     6241 caccaagaat acggttagtg agtgtagact tacctacgtt cggacgaccc acaatcgcca
     6301 gtttgatcgg cagactttgc gggtcgaagt cgtcttcctc ttcttcttcg ccgttctctt
     6361 ccacttcaaa ttgcgcccag tattcagcgt cttcgtcgac ttcctcttgc ggtgcgagat
     6421 cttccatcca cggcagcagc acatgctcca gcagacttaa tacgccacga ccgtgagacg
     6481 cggcgatcgg gtagatttca cctaagccaa gcgagtagaa atcaaccact gcctgatcgg
     6541 gatccagacc gtcagttttg tttgccacca ggaaggtcgg tttttcacgg gagcgcagat
     6601 gtttggcaat cgcttcatct gccggcatca ggcccgcgcg cgcatccacc ataaacagta
     6661 cgacgtccgc ttcttcaatc gccagcagcg actgttccgc catgcgggtt tctacaccgt
     6721 cttctgtgcc atcaatcccg ccggtatcaa tacagataaa ctcacggcct tcaatttccg
     6781 cacgaccgta cttacggtca cgagtcagac ccgggaaatc cgcaaccagc gcatctcggg
     6841 tgcgagttag acggttaaat aacgtggatt ttcctacgtt agggcgcccg acaagcgcga
     6901 ccacaggtac catgtttaaa gcctcatttt tataaatcat cagacaacgc acgctatatt
     6961 cgcgtcgttg ttaaaaacag gaaaacggcc cctgtccagg agccgttttc aaagtgaacg
     7021 acagagacga ttaacgtgta atagagtaca cggttccgtc ttttgcctgg atcagcagtt
     7081 tgccgtcagc ggcaaccggt tcagtctgga aaccggaact atcaactttt tgctgggcaa
     7141 cgaaacgacc atcttcgaca ttaatccagt gcagataacc ttcactgtca ccgaccacca
     7201 ggttgccatc atacagcacc ggagaagtca gcaggcgatg caacagatcg ctttgtgtcc
     7261 acagcgtaac gccgccatca atggtcaacg ccatcacccg gtcattttga tcgaccagat
     7321 agatgcgatt gccgtcgacg atgaaatcat tcaccgaacc cagttcgcgt ttccacataa
     7381 tctgaccact gcgcagatca agcgccgtca ggttaccatt ataggccagc gcgaaaacaa
     7441 cgccgttaac aacgacggga gtcgtgtcaa catcgctcag acggtcaatt tcggtagaac
     7501 cggtcgcctg ggaaatacgc tgctgccaaa tcatctggcc ctgttccatc agcactgcgc
     7561 tgacgcgacc attatcgccc cccacgacgg ccgcaccaaa agccgttgcc ggcgcagact
     7621 cgccacgcaa agagagcgaa ggcatatcga ggttaactgt ccatttgaca gcgccgtcag
     7681 cttcgttcag cgcttgtaac tgaccgttac tggtgtggat taacaccaga ccgtcgctga
     7741 ccaccgggcg cgaaagtgct tcacccgcga ctttagtttg ccatgccaca gtaccatcgc
     7801 tggtattcag cgcgtaaacc tgcgcctttt cgctgccaat gtagacatgc ccaccagaca
     7861 cggtcacacc gccagaaagt aatgcaggct ctttagagaa ccagccatct ttctcggcca
     7921 ggctgacaga ccagatttct ttgccatcat ccgcattcag cgcttttacc aaaccagcgc
     7981 ggtccgctgc atagacaacg ttgtccgcca gtgccggatg aagattggaa tagaagttgc
     8041 caatgccgct accaacggaa gtactccacg ccgtggtcgg cgtaaactgg ttttcaacgg
     8101 ttggcaatgg ggacatcttt accacatctt cttcgctgtt aaacagcgaa cagccgctta
     8161 aaagggtaac ggaaagcagt cctggcagca gtaatttacg caattgcatc gggtccctct
     8221 cagatggaca aattattaat tttcatctgc atcatttcgc tcagtgccgg agtaacatcg
     8281 cttttcacgc ctgcttccca tgcactacgc gcaccttgct tatcaccttt gctcagcaat
     8341 gcttcaccac gcaggtcggc aacaatggca gcccaccctt cacctttgat ggtatcaagg
     8401 gttttcagcg cggcatcagc ctgcttgagc tgtacctgaa cgcgagcaag acgcagattt
     8461 atcacggctt tgagattttc atcgctcgtg tctgccagcc cctgttgtaa ctgggcggca
     8521 gctttctcta gttcattttt gtcaacaaat tgctgcgcaa gttccaaaga agccagcgca
     8581 ccataagtat ttttattttc agcagcaaat ttttccgccg ccgggatgct atccggtttg
     8641 ccttcgctca ctgcggtaac cgcattttga taggcaagag aagcggagcg tgcagaatca
     8701 acctgatggc tgttccagta gcgccagcca atcagtgcgc caacgcccaa aatcacccca
     8761 acagccagtg ctttgccatt ttcagcaaaa aagcgtttaa ccgcttctac ctggtcgttt
     8821 tcgttctcgt aaatttccac gctgtccttc tccttcctta acccagtaac gtgcgcaaat
     8881 gcgcggctac gctatcctgc gcaactgccg tttgctcacc agagcgcaaa tccttcacta
     8941 ctgctgtgcc gttagtcact tcagactcac ccagcaccac agcaacgcgg gcaccccatt
     9001 tatcagcacg ggcaaactgt ttcttaaagt tgccgccgcc gtggttggtc atcaatttca
     9061 cgccctgtaa ttcatcacgc agacgctcag ctaatgccat agccgcagat tgtgtatcag
     9121 cacctgaagc caccaggtat atatcgacaa taggatcggc tttaaattcc ggattaacgg
     9181 cctgtactaa caatacaaga cgttcgaggc ccatcgcaaa accgacagcc ggtgttgcac
     9241 gaccgcccag ttgttccaca agaccgtcat aacgaccgcc tgcacacacg gtgccctggg
     9301 agccgagact gttagtcacc cactcgaaaa cggtacggtt atagtaatcc agaccacgca
     9361 ccagacgctg gtttacggtg taagcgatcc ccgcgctctc aagcagtttg cacagaccgg
     9421 caaaatgctc acgagattcc tcgtccagat aatcacctaa tgccggagcg tcgttgagaa
     9481 gcgcctgcac ttccggattt tttgaatcca gcacgcgcag cgggttagtg tacatgcggc
     9541 gtttgcagtc ttcgtccagc ttttctttat gctgctcaag gaatgccacc agcgcatcgc
     9601 ggtaattggc gcgtgcttcc agcgaaccga tagaattcag ctcaagagtt acgtgttcgg
     9661 aaatacccag cgcgcgccac cagcgggcgg tgagcataat cagttcagcg tcgatatccg
     9721 gaccttgcag accgaaaact tcgcagccca actgatggaa ctgacgataa cgccctttct
     9781 gcggacgctc gtgacggaac atcggcccga tataccacag acgctgttcc tgattgtaca
     9841 gaagaccatg ctcgatgccg gcgcgtacac agcccgccgt cccttcaggg cgcagagtca
     9901 ggctgtcgcc attgagatcc tcaaaggtgt acatctcttt ttcaaccacg tcggtgactt
     9961 caccaatcgc acgtttgaat agcggggtct gctctacaat cggcaagcgg atttcactgt
    10021 aaccgtagct gccgagcacg tttttcagtg tgccttcaat gcgctgccag atggccgttt
    10081 cgccaggcag gtaatcgttc atgccgcgaa tggcttgaat gttttttgcc acgtttattc
    10141 tctttctgaa tataaaaatg aaccctcaac gcttccctca atgtttgggg agccatgcgg
    10201 gttcaatcat acacgggaag cgaggcgctt cccatcacgt tattattttt caacctgctg
    10261 aacgtcaatt cgacgcgctt catccagctg actggctttc gcacgaatgc gtgcttccag
    10321 ctggtcgatc atatcgttgt tgtccagacg gtctttgcgc acgccatctt catagaggcc
    10381 gcttttcttg ttgccgccgg tgacgccgag tgtagaaacc agcgcctcac ctgggccatt
    10441 caccacgcag ccgataatag aaacgtccat cggagtgatg atatcttcca ggcgttgctc
    10501 cagcgcgtta accgtaccga taacatcaaa ttcctgacgc gaacaggtcg ggcaggcgat
    10561 gaagttgatc cctcgcgaac ggatacgcag cgatttcaaa atatcgaaac cgactttgat
    10621 ctcttcgacc ggatcggccg ccagcgatac gcgcagcgtg tcgccgatgc cttcagacag
    10681 cagcagacct aaaccaatgg cggattttac tgccccgctg cgtgcaccac cggcttcggt
    10741 gatccccaga tgcagcggct gatcgatctg ttttgccagc aaacgataag actcaacagc
    10801 gaggaagacg tcagacgctt tcacgctgac tttgaactga tcgaagttca ggcgatcgag
    10861 atgatcaaca tgacgcatgg cggattccag caacgcctgc ggcgtcggtt cgccatactt
    10921 ttcttgcaga tctttttcca gcgatccggc gttaacgcca atacggatcg gaatgttttt
    10981 atcgcgcgca cagtcaacca ccatgcgaat acgctcttca ttaccgatat tgccagggtt
    11041 aatacgcaga caatcgacgc cgtattccgc tactttcagc gcaatgcgat agtcgaagtg
    11101 gatgtcagcc accagcggca cgttaacccg ctgtttgatg agtttgaacg cttctgccgc
    11161 gtccatcgtc ggtacggaga cacggacgat atcagcgcca acgcgttcca gcgccttgat
    11221 ttgattgacc gttgcttcga catctgtcgt acgcgtattg gtcatggact gtacggcgat
    11281 gggagcacca tcgccaatcg gcacattccc aacgtaaata cgtgttgatt ttctacgttg
    11341 aattggagcc tggttatgca tgaaaaatct cccgcgttac ccgtctgtta ctgcgccggt
    11401 gattgttcgg cattgagggt cagacgcgca acctggttag ttctgataaa acgactcaga
    11461 tcgacaggtt tcccttgata ctggatctgt actgcggctg gcgcaccaat tttcagtttg
    11521 tacggtgcct ggccggttaa gttcaaatta ccgtctttac gctgcatacc gctaaacaat
    11581 tttttaccgg tagcatcagt gacctccagc cagcaatcgg cagtaaagtt catcaccagc
    11641 gcattcggat cagccaccgg cgtggtcacg ccagcctgat cggttggcaa cggcgcagca
    11701 ccatctggcg ttgttgctgc cgttggtgcc ggggtcgcgg cggtatcaac atttgcctgc
    11761 gaaggcgaaa caaccgcatt ctgttgcgga tcaacagctg gtgctggcgc agttacggca
    11821 ggtgtttgcg tgttggttgc ggtagtatcc acagacgctg gcggcgtgga cgttgtagcc
    11881 gggtctgtag ttgtcgacgt atttaacgga acactctgcc cctgctcgct attactgctc
    11941 agttccgccg aagattgatc ggccatagtg gtgatctctt cctgctgagc tttgtggtct
    12001 tgccaccacc aggcaccgct caggccgata accacaaaca acaccagcca ggtgaaggtc
    12061 atcagccagc cgtcacgttt tttgcggcgt ttaccgaggg aaaaactctg catcggcgca
    12121 actttcgcag cccgtagtgg agcctgcttt tccagcccta gcagcagttc ttcttctgga
    12181 atatgtacca gacgcgcata agagcggata tatccgcgca ggaatgttga agcaagatcg
    12241 gcgggtgcct tatcttcttc aatgtcgcgt accgtggaaa ccttcaggca aagtcgctcg
    12301 gcaacggcct gctgactaag tcctagttgt tcgcgagcat tacgcaggcg agcgccggta
    12361 gtaagtgctt cattttggtc gtgcgtggct tcagtattca ttcgctgcag gtacgtttaa
    12421 atgagaattg agatgccggt gaaccatcat gctcacccac accgcgaaac atccgttaag
    12481 ttaaccgtta tcatacagta taagaccgtc tgtgtgctcg tgacaaagcc cacattcact
    12541 aaggctaact tactgttgca tcgttacata ctgccttaaa gtcagcaaaa acgcaccgtt
    12601 aatattgacc agacaattgc aacttaatac ttcattcata aaagattacg ccacggtaca
    12661 taaagtaacc gtggcgtaat ggctatcaga ccgctttaat gtcgatggct tcaccctgca
    12721 tccgtttacg cagggtacgt ttcgtacggt cgataacatc gcccgccaac tgaccacagg
    12781 cagcatcgat atcatcacca cgagttttac gcacaatagt ggtgaaaccg tagctcatca
    12841 gcacttttga gaaacggtcg atacggctgt tcgaactgcg tccatacggc gcgcccggga
    12901 acgggttcca cgggatcagg ttgatcttac acggcgtatc tttcagcagc tccgccagtt
    12961 ggtgcgcgtg ttcagtgccg tcgttaacgt ggtcaagcat cacgtattca atagtgactc
    13021 ggccctgatt ggcgttggat ttctccagat aacggcgcac cgcagcaagg aacgtttcga
    13081 tattgtactt tttgttgatc ggcacaattt cgtcacgaat ttcgtcgttc ggcgcgtgca
    13141 gggaaattgc cagtgcaacg tcgatcatat cgcccagttt atccagcgcc ggaactacac
    13201 cggaagtaga aagcgtgacg cgacgtttag acaggccaaa accgaaatca tccagcatga
    13261 tttccatcgc cggaacgacg ttgttcaggt tgagcagcgg ctcgcccatg cccatcatca
    13321 ctacgttagt gattgggcgc tgaccggtga cttttgctgc gccgacgatt ttcgccgcac
    13381 gccacacctg gccgataatt tccgacaccc gcaggttgcg attaaagccc tgctgggcgg
    13441 tggaacagaa tttacactcc agcgcacacc ccacctgcga agagacgcag agcgtggcac
    13501 ggtcgtcttc cgggatatac accgtttcga cgcgctgatc gccaacggcg atcgcccatt
    13561 taatggtgcc gtcggatgaa cgctgttctt caaccacttc cggtgcgcgg atttccgcca
    13621 cctctttcag tttgccgcgc aacactttgt tgatgtcggt catctcatca aagttgtcgc
    13681 agcaatagtg atacatccac ttcatcacct gatcggcgcg gaagggtttt tcacctaaat
    13741 ctttaaaaaa ctcccgcatc tgctgacggt tgagatccag caggttgatt tttccatctt
    13801 tcgtggtgac gttttcaggt gtgactaatt gttcagacat atgctattcc ggcctcgtta
    13861 ttacacgtta tggcccctgg agggttgaaa aaagaaacgc cccggtgagc ggctgctcgt
    13921 ccgggggcgc tgcattgtac aaattctggc gcacggatgc cacgtttgca cgcggcattt
    13981 acgaaattat taacgggtgc gcgggcacac ttcgccttcg ccaaagaaat aagcgatttc
    14041 gcgagcggca gattcgacgg aatcagaacc gtgggtaccg ttttcggtca ggctgtcagc
    14101 gtaatcagcg cgcagagtac cagccagtgc gtttgccgga ttggtcgcgc ccagcagatc
    14161 gcggtgacgc tgaacggcgt tttcaccttc cagcacggaa accacgatcg ggccagaggt
    14221 catgaattca accagaccat caaagaacgg ttttccatcg tgttcagcat aaaagccacg
    14281 tgcctgttca acggtcaggt gcagcatttt ggtgccgaca attttgaacc ctgcagcttc
    14341 aaagcgcgca aagatattac caatgacgtt ttttgctacc gcgttcggtt tgatgatgga
    14401 aaaagtacgt tcaatagcca tttttacctc tgtaaattgt tctgttgttg tctgtaccag
    14461 cgtacgagat ggcgcggatt ataatgagca acagggccgt tgactattga tgaaggtaac
    14521 atttttttaa aataaaatga ggtttagcaa caatcaggga caaaacagac tattgcatga
    14581 caaatttcac tgtcgcgatt tgccccacgt catccatcac cagcaattga taatcacctt
    14641 tatccgtcaa atgcagggtc acgttgcgcc cgcgttcagt taacggttcg ccattcagaa
    14701 accaccagcg ttcacctgcc cctccacttg attgcaacgg caaagtcgct tccgctgcgc
    14761 ccggtaaacg tttaataatc gcgccatcgc gaacgcctgt cagctgcagc gggagctggg
    14821 catagtgacc gtatggcggg caacttgtcg aggccggtgg taagcgcaca gcgcgacgtt
    14881 ctgatgcggg cagccagggt tccagcggta gcggccagac atttatcatt tcttgtcgcg
    14941 cttgcgggca atcagcggca acacgtttgc cattttcatc cagccagatg gggaaacgaa
    15001 tgccattaat gccttcctgc tccggcagta atagagttgg cggctgactc ccgtccagca
    15061 gccaggttgc caggcggcgg cgacagttac cgtcaccttc cggcaaagac tgtccgcccg
    15121 gccagcagat aacgccacga gtgactgagt tcgggcgcgg gtcttccggc agattcgcac
    15181 tgcgcgacag taagatatta ttgacctgat tcaacaatgg tacggcactg gcaaagccaa
    15241 actgaccaac aacgggcgtg ccgtccggtc tgccagtcca gatcccaatg acatagcgag
    15301 cgttaacccc aatcgcccag gcgtcacgat agccatagct ggtgcccgtt ttccatgcca
    15361 gtggggcgac gcgcggcaag gcactatccg gcaagggttg cgcttcatca gccataatcc
    15421 ggcgaatgat ccacgccgcc cccgacgaca ttaaaggcca ttcaagcagc ggatcgtcag
    15481 gctgtaagcg caatttgcct gccttgccgt ggcgagcaaa cgcggtatac gctgccgcca
    15541 tatcttccag ttttgcacca gcgccgccga gaatgagtga aagattcggc gcagcaccgt
    15601 tgggcaaata taacggcaat ccaacattgc gtaactttgc cgcaaaccgt ttcggtccat
    15661 aggcttccag cacctgcaca gcaggtaagt tcagcgagcg caccagcgcc tcgctcatgc
    15721 tgatcgggcc atgaaaaccg ctatcaaagt tacctggtcg ataatcaccg gtgcgccggg
    15781 ggacgtcttg cagcagtgat gccgggtgga tcaagccttc atccagcgcc agaccataaa
    15841 caaacggttt gagcaccgat cctggcgatc ggatcgcatt gaccatatca acatgaccaa
    15901 agcgtgaatc atcgttgaga tcaaccgatc ccacccagcc gcgaacacgc atatcggtat
    15961 gatcaaccac gatcatcgcc agtgagctgc gcggtggcaa ccgccctttc cagttttgcg
    16021 ccagttcttc cagacgtcgt tgaagaccgg catccagcgt agtaacgatt ttgtcgcttt
    16081 tgcttttacc gagcatcatg cgcgaaaaca gcggtgccag ttgcggcatt tgtcggggtg
    16141 ccagccagat gggttcttcc cttgactctt ttacctgctc acgggaccac acaccttgca
    16201 cggccatccg ttcgagcact ttattacgcg cggcttcggc acgctccggc caacgatccg
    16261 ggcgcagtcg gctgggcgct tgcggtaaca ccgccagcat tgccgcctcg gaatagctta
    16321 aattcgcagg cgattttccg agataagccc aacttgccgc accgatcccc tgcaacgtac
    16381 cgccaaacgg agcgcggtta agatacaaga tcagaatttc acgcttagac aaatgccact
    16441 ccagttgcaa cgcgcgccag agctggagaa ttttgccacc aaatgttttg gggtgaggat
    16501 caagcagacg agcaacctgc atagtgagcg tgctgccacc agaaataacc cgtcccgaag
    16561 tgagatcttg ccatgctgcg cgcgccaccg agaatggatt cacccccgga tgcttccaga
    16621 accagcgatc ttcataattg atcagcgctt caaggtaacg tggagacaca tcttcgattg
    16681 ttaccggata acgccagatg ccatcagcat cggcgaagcg ccagagcggc gtaccatcct
    16741 gcgccacgac cactcgtgcg ggattgactt catgcagcgg tagaggccag agtttatctg
    16801 ctccccacgc tgcaagaaaa aggagaaatg gcacagctgc cagcgttatc cagcagccgc
    16861 gtttggttaa caagcgaagc atttacggtc ggacaatcag cagatcttca gccgcgccgg
    16921 tcgcccgcca ttggggaaca tacattgatt ccaccatcgg ttgcggtacc tgatacgttc
    16981 ccggcgtcac cgcccgcgcc agatacacca gcgttaccgg ttggtattca tcaacggcaa
    17041 ccgccgccac aaagcgatcg tcacggaact caatgtgctt aatgctcgcc tgctgcatct
    17101 ggttcagtaa gttttgcact tcgtcaccac tttgctccag gctggcgcta ccgttcgcca
    17161 gattctgatt ttccagttcc agacccgcag gcagcagatc cacgactaac gcatccggca
    17221 cgctgttact ggcttttacc tgcaaccaga ccagcaccag atcgccgcta cgtaacgagt
    17281 ccagcgattt gctcttacca tcagtaccaa gaatatgacg ctcgatttgc agcacattgt
    17341 tcgccggtaa aggtgcggat tgcggataac cgctggcatc catacgcaac cataacggct
    17401 gatcgccact gtttctcacc tgcaaggtgg caagttgatc gctattcaga ttgctgttta
    17461 gcgttttctc acctgtcagc ggctcagctg agaaagaggt ttgcgcctgc catttacccg
    17521 gtaaatcctg aatcgtacgg gcagccagga acaacgcgtt actttcctgc gtcgatagcc
    17581 agcgttcacc aaacgcctgc tgcgaaagtg tgttcagcaa agtgtactgc tcatcgggta
    17641 gcagtttatt ttcttccagc aaggagagca ttaacgcgtt gtcgcgcagt ggactaccgt
    17701 aatcacccag ccatatccgc tcatcactat ttctcggcgt tttcagcgcc agcacaatcg
    17761 cttcttcacc acgcgttgca tcacccatgg ttttcagcgc aatgccaagt tgcagcagcg
    17821 gtaaaccaga agcggcatct gcacgatgct cccagatttc acgcagcgca cccagtggtg
    17881 ctttttgctg acgggccagc accagcgcag cgtaagactg aacggcgaat ttactggctt
    17941 tgagattatc cgcgtacggg atcgacatca tgcccggatc ttgtaaatag cgcagcagac
    18001 gctcattacc ccggttaatg gcgtctgtcg gcacgctgta accctgttcg cctgcgcgga
    18061 ccaggaaatc catcacgtaa gccgtcagcc agtactcttc gtcaccgttt ttatcccaca
    18121 gcgcaaagcc gccgttatca cgttgcattt gcagcaaacg ggaaatgccg atatcgaccg
    18181 atgcacggcg tttctcatca ctgtcgcctt tgatgcccaa cgcctgcagt tgggctgcgt
    18241 tggtataaag tgacgggaac aggccgctgg cggtttgctc aagacagccg tacggatacg
    18301 cttttaactc tttgatataa cgtgcaatgt tcagcggtgg tttgccgctc aacaacaatt
    18361 gcccttccag cgtaacaggc gagaagtttt gcaatccatc cgccggaatc gcccatgtct
    18421 caccaggctg taacgccgta ccgtaattaa ccgtttgtgc cgggaacgcc ggacggacgc
    18481 cgattttcca ctgcttatgc tgatcggcaa cggtttcacc cggtaacgct aacccgctaa
    18541 tggtggcctg aatttctcca tcgccataac ccggcaatgc tcgcaccggg ataaacagcg
    18601 tagtacgcac acctggcgct aattcaacgg ctgcgggtga atcgctgacc agttcaagca
    18661 aaccactggc ggtcagggca acgttcagtt tttgcggttt atcggtaaga ttagtgatat
    18721 ccagcgtcag acgcgaggta tcgccactcg ccataaagcg cggcatgttc agttcagcaa
    18781 tcaccggtgc ggcaactatc actttgcttt cgttgctacc gaaatcatct gccgtccagg
    18841 cttgcgccat gacgcgcaat tcaccgttaa aatcgccaat cggcagtgta accgagcctt
    18901 cgccctgttc gttgagcgtt accggcagcg cctgctgcgc gacaatattg acgtgattga
    18961 ccagcggttt accaccacgt ttcagctcat caccatcgcc accgaaacgc agagctgcca
    19021 gacgcccctg accttcaata acctgaccgt aaatatcgta aatgtctgcg ccatagcgtt
    19081 tctgaccaaa gaacgcctgc cacggatctg gcgtgacgta gtcagtaata ttcagcacac
    19141 cactatcaac ggcagacacc agcacattca cctgtttagg cttctcgcca tttttagtgc
    19201 tggctttaat tttcacggtt aatggttgat tgggacgcat ttttgctggt gtttccagcg
    19261 ccagatcgag gcgacggttt tcatcgccaa gcggcagatg caacacacca accgcgcgtt
    19321 ttggcgtcgc ggagcgagat ttatcgctag gacgtaccac cagcgtactt aaatacagat
    19381 catgacgatt ccaggtttta tcgaccggaa tcgtcagatc cagcccttga gccggaacat
    19441 caatctcttg ccaccacagc ggcccttcac tggactcgac catcgcataa cctttacccg
    19501 ccgttggcgc ggcgatatgc aacttaatgg tgtcgccagg gcgataactg gctttatcca
    19561 gtttcagcgt gacacggtcg ggtcgcactg cgccgctacc gtcgctgttg tcctgccaac
    19621 tatagccagc ccagaaacga acactactga ccgcttcatt cggcgctttg acttccagac
    19681 gataagcgcc ccactctacc ggaaaactga ccttgccggt ttcgtccgct ttcagatcca
    19741 gagtttgttc attttcgatc agatcttttt gatcaaactg tgactgccag ccttcatctt
    19801 ctgaccagtt ccagtagtaa tcgcggcgtt cgcgaatcag gcgcacctgc aagcccgaca
    19861 cggctttttt cacgccttgt gcatcgctat aaacgatgtc aaaagcggcg ttactgcctt
    19921 catcaacaat gggctgtttc accgtgctgt cagtgcgata atcgtaaacc gatttcaagg
    19981 cgaactgcgg acggagcccc ggcaatgcat tggcaggcca gatagcctgc tcagcgcggc
    20041 gcgtcaccgg gcgaccgccc gattccagca aactgccctg gaaaataacc tgcaatgggg
    20101 aatgcgtttc cttccactgg ctttctgtag aaacttcgcc gcgcccttta tcatccagcg
    20161 tcaactgaac ttcatccagc gtgcgggaaa ggttttcggc agctatatcg ccgaattcaa
    20221 aaccaggtaa ggctgacaca gcctcacgca gtgggcgcag gaaaagttgc ccttgcaaag
    20281 tattaccatt agcaggcgca ccgtacaggt agtatcccac cacggagaat ttcacttcat
    20341 cattcggcgt aagcggggtt ttctcaccgg tcagattcag cgccatacgc tctggcataa
    20401 aatcttcgac gtggaaatcc cacatccggt actggttatc gcccgtgttg gcgcgaatat
    20461 gccacatacc ggttgccgca ttgctatcga gtggccaggt aaagtggtag aggccattct
    20521 ccggctgact aacgacgctc ctgagtacct gcccgtcagg tttaatcacg tctaacttga
    20581 tgggttgatc gggcaatgct ttaccgtctg catcgcgcag taaaccattg aggattaccg
    20641 tttcacctgg gcgataaaga tcgcgtgggc caaacatgaa aaactgtttg ctatagcctg
    20701 gcgcgccagc aatgttaaat tctgctaagt ccagcgccgg aagttttaaa tcgagtagcg
    20761 ttgtctgacc gtctttacgt gccagcagta atgccgcatt tttatcattt tccagctgca
    20821 catgcccctg agcgtcactg gttgcctgag tcagagtctg ccctttctca tttaataaag
    20881 agacttcaat tccttgctgg gccgcgccgt tttccagact ttgggtaaag atatccagac
    20941 gattgtgata acggtgagct gaaacgccga tatcacttaa cgtaaacagc gtcgcgggat
    21001 tactgtaatc gtaacgtcca gcctgattca tcacagccag atacacgccc gcctgctgaa
    21061 gcggtttgat atcgcccagc ggcagcaata atttttcacg ggtgttacgc gcaggattga
    21121 gatcaaaccg tccggtgtag accagatccg ccatctgcag cagtttgtct gactgccagt
    21181 tcgccagcga attgcggtat tcccattggc taatgaatgc tggcagagat tctggcttaa
    21241 cgcggaagaa gttaacatcg acattattaa cgttgagcgc cattaccggc agcccttcaa
    21301 cgactttgcc aggcagcagc gaaccacggc tggcaaaacc gacgctgggt tggatgtcgc
    21361 gggtagttat agttttttcg taatctttac tgaaggttgc gttgttgagt gctttgacct
    21421 ccttgccaat agtaacgatc aaatcacgtt tcggttcgag gtggcgtaaa cgtagctctt
    21481 taagattatc tgacagctcc caggcaccat ccactttgcc gcttttttta tcgacgacat
    21541 gaataacgcg tgagaaatcc tgatccgggt cgagagggat ggagaacgtc agcaccagcg
    21601 tggctgcacc atcaagttgg acttcagaga gatccagcaa tgtcagcacc ttaccggcac
    21661 tctgttgggc cagtttttgt ctctccggca cggagagttt tgctgaactc gcgttttctg
    21721 aag
//
LOCUS       DAJTSL010000060        20754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTSL010000060 DAJTSL010000000
VERSION     DAJTSL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.060"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(11..556)
                     /gene="hemG"
                     /locus_tag="OO433_002018"
     CDS             complement(11..556)
                     /gene="hemG"
                     /locus_tag="OO433_002018"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6050508.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OO433_002019"
     CDS             complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OO433_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6050509.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2058..2672)
                     /locus_tag="OO433_002020"
     CDS             complement(2058..2672)
                     /locus_tag="OO433_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6050510.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGIGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OO433_002021"
     CDS             complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OO433_002021"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6050511.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHLQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFSGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4193..6382
                     /gene="fadB"
                     /locus_tag="OO433_002022"
     CDS             4193..6382
                     /gene="fadB"
                     /locus_tag="OO433_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6050512.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6392..7555
                     /gene="fadA"
                     /locus_tag="OO433_002023"
     CDS             6392..7555
                     /gene="fadA"
                     /locus_tag="OO433_002023"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6050513.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCN
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7747..8448)
                     /gene="fre"
                     /locus_tag="OO433_002024"
     CDS             complement(7747..8448)
                     /gene="fre"
                     /locus_tag="OO433_002024"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6050514.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDYQIVVDIPYGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLRVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8494..9987)
                     /gene="ubiD"
                     /locus_tag="OO433_002025"
     CDS             complement(8494..9987)
                     /gene="ubiD"
                     /locus_tag="OO433_002025"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6050515.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGVPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10154..10642
                     /gene="rfaH"
                     /locus_tag="OO433_002026"
     CDS             10154..10642
                     /gene="rfaH"
                     /locus_tag="OO433_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6050516.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10639..11421)
                     /gene="tatD"
                     /locus_tag="OO433_002027"
     CDS             complement(10639..11421)
                     /gene="tatD"
                     /locus_tag="OO433_002027"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6050517.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDFTPKPSS
                     RRNEPAHLHHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11463..12239)
                     /gene="tatC"
                     /locus_tag="OO433_002028"
     CDS             complement(11463..12239)
                     /gene="tatC"
                     /locus_tag="OO433_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6050518.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12242..12757)
                     /gene="tatB"
                     /locus_tag="OO433_002029"
     CDS             complement(12242..12757)
                     /gene="tatB"
                     /locus_tag="OO433_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6050519.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELIQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12761..13030)
                     /gene="tatA"
                     /locus_tag="OO433_002030"
     CDS             complement(12761..13030)
                     /gene="tatA"
                     /locus_tag="OO433_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6050520.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13109..14749)
                     /gene="ubiB"
                     /locus_tag="OO433_002031"
     CDS             complement(13109..14749)
                     /gene="ubiB"
                     /locus_tag="OO433_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6050521.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14746..15351)
                     /gene="ubiJ"
                     /locus_tag="OO433_002032"
     CDS             complement(14746..15351)
                     /gene="ubiJ"
                     /locus_tag="OO433_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6050522.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15365..16120)
                     /gene="ubiE"
                     /locus_tag="OO433_002033"
     CDS             complement(15365..16120)
                     /gene="ubiE"
                     /locus_tag="OO433_002033"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6050523.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16215..17642)
                     /gene="rmuC"
                     /locus_tag="OO433_002034"
     CDS             complement(16215..17642)
                     /gene="rmuC"
                     /locus_tag="OO433_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6050524.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17783..18544)
                     /gene="udp"
                     /locus_tag="OO433_002035"
     CDS             complement(17783..18544)
                     /gene="udp"
                     /locus_tag="OO433_002035"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6050525.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18806..19618
                     /locus_tag="OO433_002036"
     CDS             18806..19618
                     /locus_tag="OO433_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6050526.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGMHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            20012..20672
                     /gene="hcp"
                     /locus_tag="OO433_002037"
                     /pseudo
     CDS             20012..20672
                     /gene="hcp"
                     /locus_tag="OO433_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680647.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5080 a         5604 c         5210 g         4860 t
ORIGIN      
        1 ttctttatgc ttatttcagc gtcggtttgt tggttaactg ggcgatttct cgggcgaaat
       61 tcgccacctg ctcccaatcg gtatagacaa cttctttgcg cgtatccgtt tcaccgcctg
      121 acatcttcat aatcagctta atcataaaac ggtcgtacca gcgataacgt gggtaacgca
      181 gcgccccggc aatgaccgcg cagcgatcgg gacgccattg tgaactcatc agaaacttgc
      241 gcgcgtagct gttggtctgt ggagtacgct tctccggttt gcgcgccacc aggtttacgg
      301 aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg ttttttgaca aattcctgga
      361 acgcagagtg gtagtgacca tagcgaatag aagcaccaat gaccacacgg tcatagtttt
      421 cccattgtgg ttcttcaatg cggtgcacat tggcgacatc cgtctggatc cccagttctt
      481 tcagttccga agccaggtag gaggcaatct cgcgcgtttg tccgtccctt gttgagaaaa
      541 gaattaatgt tttcacgtat tactccatca ttcacgccag aaagtcgggg taaagagcac
      601 cagcaatgta aagacctcga gacgaccaaa cagcatgttg gcaatcagga tccatttagc
      661 caccgggttc atactggtaa agttatcagc aaccacgcca agccctggcc ccaggttatt
      721 caatgtcgca acaactgagg caaaggcaga aaaatcatcc acgcccgtgg cgataatcgc
      781 cagcatactg acaataaaca ccaatgcata ggcggagaaa aatccccaaa cggcttcgag
      841 gatacgttcc ggcagtgcgc gattccccag cttaatgcta tagacggcgt tcggatgcac
      901 cagtcgtttc agctcacggt tcccctgctt aaacagcagc aggatgcgga tcactttcag
      961 gccaccgccc gttgacccgg cgcaaccgcc gataaaagct gaacataaaa gcagtaccgg
     1021 caaaaagagc ggccagcggg caatgctgtc agtcgtaaac ccggcggttg tcgccatcga
     1081 taccacctgg aaaaacgcct ggttaattgt catcagcgcc gaactgtaga cattatgaaa
     1141 ccacagtacg agtgtacaaa taaccaccag cgtaaactgt acgccgataa acatgcgaaa
     1201 ttccggatcg cgccaataaa ccttcagact acgcccactt aacagtgaaa agtgcagacc
     1261 gtagttacaa ccggagatca gcaggaagat agcaatgata gtgttaatag tcggactgtc
     1321 gaaataaccg atactggcat catgtgtcga gaagccgcca atagcgatag tcgcaaagct
     1381 atggccgatg gcatcaaagg catccattcc ggcaaaccac aacgccagcg cacaggcgac
     1441 ggtcagcaag acataaatca accacagggt tttcgccgtt tccgcaattc gcgggcgcat
     1501 tttgttatct ttcagcgggc cgggcatttc tgcgcgatag agctgcatcc cacccacgcc
     1561 gaggataggc agtatcgcaa ccgctaacac gatgatcccc atcccgccaa accattgcag
     1621 catctggcga taaaagagga tggcatgagg gagcgaatcc agccccacca gcgtagtggc
     1681 cccggtggtg gtcaggccag agaaagattc aaaaaacgca tcggtaatcg tgaggttcgg
     1741 gctttccgag aagataaaag ggagcgcacc gacactaccc agcacggtcc aaaacagcac
     1801 cactatcaga aacccctcac gggatttcag ttcgcccttc tctttgcggt tcggccacca
     1861 cagcatagag ccaatggcga gggcgacaaa aaaggtctgg gtaaaagcgc ggcccgctcc
     1921 atcccggtag atgagtgcta ccagcccagg gataatcatg gtccctgaaa ataagatgac
     1981 cagtagtcca acgattcggg taatggcgcg aaaatgcatc tctgccgctt ccttagttct
     2041 gcaaaatgaa gtggggatta ttcttcaatc gctaacaatt gcaatgaacc acggctaaaa
     2101 tccgccagct ttgcggaaaa ttcagccact ttcgccgccg gaagcgccac ccgcagcaga
     2161 acgaatgcct gataatcact gttgataatt ttgccgtcac actgccccag caacgcttca
     2221 atgccggtta actgactata ttcacattgc aaagtatatt cggttaatgg cgtcttgcgt
     2281 tgggtcgtta gctggcgcag cgcctgattc acgccgccgc catacgcttt cactaaccca
     2341 ccggtgccaa gcaatatgcc gccgtagtag cgcactacca cagcggtaat ttccccgatg
     2401 ccgctgccca ttagctgggc gagcatcggt ttacctgccg ttcccgccgg ctccccgtcg
     2461 tcagagaaac ccagctgttg agaatcatcc ggcgcacccg ccacccacgc cacgcaatgg
     2521 tggcgggcat cggggtgttc tgcccgcacc gattcaacaa acgctttcgc cgcctcaacg
     2581 ccatcggtat gcgccaacat cgttatgaag cggctctttt tgatctcttc aacgaccgtg
     2641 accggtgccg caggaattaa ccagctttcc atcacgccag tttcagatcc cgggtcatgt
     2701 tttccacgtt attttcgtgg atcaccacgt tgtcttcgat acgaatgccg ctgaacggtt
     2761 tcagtgcttc aattttctgc cagttgaagt gcttgctgaa ctgcccttca cgccacggtg
     2821 ccagtagcga ttcgatgaag tagatacccg gttcgatggt taacaccatg cccggctgga
     2881 gaatacgggt gcagcgcagg tacggatatt ttgccggtgc cgcgaggtgt gtaccgctat
     2941 catcctgcat aaaaccggct acgtcatgca cctgcaggcc cagcggatgg ccgataccgt
     3001 gcggcataaa cggtccggtg agatcgtttt cgaccatcgc ttcttcactc atatcggtga
     3061 tgatttgatg tttacgcagc aatttggcga tgcgctgatg gaactggagg tggtaatcca
     3121 catagctgac gccagctttc atggtagcga tcagcgcaag ttgttcatca tttacgtctt
     3181 tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca ggtacgagtc aggtcagccg
     3241 catagccgtt atattcggcc ccggcatcca gcaggaagct gcgcatctct tccggtgcct
     3301 gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc gttaagcgcc acaatgttgc
     3361 tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag ataggcgatg ttgatatcga
     3421 actcgctcat gccagaacgg aacgcttctt ctgccgcacg atgaccgttg accgccattt
     3481 tctgcgcttc acgcatacag gccagttcgt actcggtttt gaaggagcgg tagtaatgca
     3541 ggtagtcgat cacccctttc gggttgatgt tgctggcctc aatacccagt tgcagcgcac
     3601 gttccggcac cggaccgata taaccgatat tgccgcgcgc agcgggcagc aggctaccaa
     3661 tgccatcggc tttcggcagt gcgatcactt ccacatcttc agtccagaag gaggtcggca
     3721 gcggttcgac gttgtgccag taatcaaccg gcagatagaa ccacagtttc ggcttgttca
     3781 cgccatccac cagcaaccag cagtttggca cctgagttac cggcacccac gctttgaatt
     3841 gcgggttcac tttaaacgga tagggatgat cgtcgagaaa aacgttgaac agctcgccgg
     3901 agtgaataag taatgcatcc agcttgaagc gcgccagcgc atcgcgagtc cgttcttgta
     3961 aggtagctat atgattttta tagagcgagg ccagtgattc cattttttac ccttctgttt
     4021 ttttgacctt aagtctccgc atcttagcac atcgttcatc cagagcgtga tttctgccga
     4081 gcgtgatcag atcggcattt ctttaatctt ttatttacat gtttttaaca caaaatacac
     4141 acttcgatgc atctggtacg accagatcac cttgcggatt caggagactg acatgcttta
     4201 caaaggcgac accctgtacc ttgactggct ggaagatggc attgccgaac tggtatttga
     4261 tgccccaggt tcagttaata aactcgacac tgcgaccgtc gccagcctcg gcgaggccat
     4321 cggcgtgctg gaacagcaat cagatctaaa agggctgctg ctgcgttcga acaaagcagc
     4381 ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg ttcctcgttc ctgaagaaca
     4441 gttaagtcag tggctgcact ttgccaatag cgtgtttaat cgcctggaag atctgccggt
     4501 gccgaccatt gctgccgtca acggctatgc gctgggtggt ggctgcgaat gcgtgctggc
     4561 gaccgattat cgtctggcga cgccggatct gcgcatcggt ctgccggaaa ccaaactggg
     4621 catcatgccg ggctttggcg gttctgtacg tatgccacgt atgctgggcg ctgacagtgc
     4681 gctggaaatc attgccgccg gtaaagatgt cggcgcggat caggcgctga aaatcggtct
     4741 ggtggatggc gtagtcaaag cagaaaaact ggttgaaggc gcaaaggcgg ttttacgcca
     4801 ggccattaac ggcgacctcg actggaaagc aaaacgtcag ccgaagctgg aaccactaaa
     4861 actgagcaag attgaagcca ccatgagctt cactatcgct aaagggatgg tcgcacaaac
     4921 ggcggggaaa cattatccgg cgcccatcac cgcagtaaaa accattgaag ctgcggcccg
     4981 ttttggtcgt gaagaagcat taaatctgga aaacaaaagc tttgtcccgc tggcgcacac
     5041 caacgaagcc cgcgcactgg tcggcatttt ccttaacgat caatatgtaa aaggcaaagc
     5101 gaagaaactc accaaagacg ttgaaacacc gaaacaggcc gctgtactgg gcgcaggcat
     5161 catgggtggc ggcattgctt accagtctgc gtggaaaggc gtaccggttg tcatgaaaga
     5221 tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc gcgaaactgc tgaacaagca
     5281 gcttgaacgc ggcaagatcg atggtctgaa actggctggc gtgatctcca cgatccaccc
     5341 aacgctcgac tacgccgggt ttgaccgcgt ggatgttgtg gtagaagcgg ttgtcgaaaa
     5401 cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa aaagtacgcc cggataccgt
     5461 actggcgtct aacacttcaa ccattcctat cagcgaactg gccaacgcgc tggaacgccc
     5521 ggaaaacttc tgcggaatgc acttttttaa cccggtacac cggatgccgt tggtagaaat
     5581 tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa gttgtcgcct gggcgagcaa
     5641 gatgggcaag acgccgattg tggttaacga ctgccccggc ttctttgtta accgcgtgct
     5701 gttcccgtat ttcgccggtt tcagccagct gctgcgcgac ggcgcggatt tccgcaagat
     5761 cgacaaagtg atggaaaaac agtttggctg gcctatgggt ccggcatatc tgctggatgt
     5821 cgtgggcatt gataccgcgc atcacgctca ggctgtcatg gcagcaggct tcccgcagcg
     5881 gatgcagaaa gattaccgcg atgccatcga cgcgctgttt gatgccaacc gctttggtca
     5941 gaagaacggc ctcggtttct ggcgttataa agaagacagc aaaggtaagc cgaagaaaga
     6001 agaagacgcc gccgttgaag acctgctggc agaagtgagc cagccgaagc gcgatttcag
     6061 cgaagaagag attatcgccc gcatgatgat cccgatggtc aacgaagtgg tccgctgtct
     6121 ggaagagggc attatcgcca ctccggcgga agcggatatg gcgctggtct acggcctggg
     6181 cttccctccg ttccacggcg gcgcgttccg ctggctggac accctcggta gcgcaaaata
     6241 cctcgatatg gcacagcaat atcagcacct cggcccgctg tatgaagtgc cggaaggtct
     6301 gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg gttgagccag cccgtccggt
     6361 tggcgacctg aaaacggctt aaggagtcac aatggaacag gttgtcattg tcgatgcaat
     6421 tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt aacgtgcgtg cagaagatct
     6481 ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg gcgctggaag cggcggccct
     6541 cgacgatatt tactggggtt gtgtgcagca gacgctggag cagggtttta atatcgcccg
     6601 taacgcggcg ctgctagcag aagtgccgca ctctgtcccg gcggttaccg tcaatcgctt
     6661 gtgtggttca tccatgcagg cactgcatga cgcagcacga atgatcatga ctggcgatgc
     6721 gcaggcatgt ctggttggcg gcgtggagca tatgggccat gtgccaatga gtcacggcgt
     6781 cgattttcac cccggcctga gccgcaatgt cgccaaagcg gcgggcatga tgggcttaac
     6841 ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa atgcaggatg cctttgccgc
     6901 gcggtcacac gcccgcgcct gggccgccac gcagtcgggc gcatttaaaa atgaaatcat
     6961 cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag tttaattacg acgaggtgat
     7021 tcgaccggaa accaccgtgg aagccctcgc cacgctgcgt ccggcgtttg atccagtaaa
     7081 cggtacggta acggcgggca catcttctgc actttccgat ggcgcagctg ccatgctggt
     7141 gatgagtgaa agccgcgccc atgaattagg tcttaagccg cgcgctcgtg tgcgttcgat
     7201 ggcggtcgtt ggttgtaacc catcgattat gggttacggc ccggttccgg cctcgaagct
     7261 ggcgctgaaa aaagcggggc tttctgccag cgatatcggc gtgtttgaga tgaacgaagc
     7321 ctttgccgcg cagatcctgc cgtgcattaa agatctcggg ctaatggagc agattgacga
     7381 gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat ccgctgggct gttccggtgc
     7441 gcgtatcagc accacgctgc tgaatctgat ggaacgcaaa gacgttcagt ttggtctggc
     7501 gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg tttgagcggg tttaaccact
     7561 tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc ctgcaccaat gtaggcctga
     7621 taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg acatagatct
     7681 aaatgccact gaataaagtt ttagttgccg ttcttcccgc ctgtcagggg cgggtttttt
     7741 tatatctcag ataaatgcaa acgcatcgcc aaacagacga tcttcccgcg cattacgctc
     7801 actgcaaaac aggtcacgag caattttcgc catctcaaaa cgtccggcaa tatagatatc
     7861 atgctctgcc agcgtaccat gatcctgcaa gaccgccgtt aacacggtgc cagtacgccc
     7921 acgccagccc gcttccggtt gttcaaccac cggcaccaca cgcaggccag gatgcttcag
     7981 cgaaagcgcc tcaagctcgg agagatcata aagatgttgc tcctcacgcc caccccagta
     8041 aatggtgata tcgcgattgg gattacgcgc caacgccgtc aataaaatcg agcgcgcata
     8101 ggagaacccg gtgccgcccg caatcaaaat catcggacgc tcttcatcat cgcgcagcca
     8161 cgcttctccg tagggaatat cgaccacgat ttgataatct ttgaggatgc ggtccatgac
     8221 tgctttcgcg taaaggttga tttcagaagc gccaatatgc agctctataa accctttttc
     8281 atccggcgtc gaagccattg aaaacggacg tttgtcgcgc tcatccatca ctaccatcaa
     8341 atactgacca gcacgaaaag aaaaggccgc gtctggcacg atgcggacac gatatacggt
     8401 atccgtgata gcttctaccg aggtcacttt acagcttaag gttgtcatgc gctttctctg
     8461 tcggatcgat aaatagggca aaacaaacgc gcatcaggcg cttttaccgt tgttaaaaat
     8521 agccagttca tcccagatgg cgtcaatatg cgcgacaaca tctggatctt ttttgatggg
     8581 acgtccccat tcacgctggg tttcccccgg ccatttattc gtggcatcca gccccatttt
     8641 tgaacccagc ccggagacag gcgaggcaaa atccagataa tcaataggcg tattttctac
     8701 cagaacagta tcccgcgccg ggtccatacg ggtggtaatc gcccaaatca catcgttcca
     8761 gtcgcgtgcg ttgacgtcat catcgcaaac gatcacaaat ttagtgtaca taaactggcg
     8821 taagaacgac cagacgccca tcatgacgcg cttcgcgtgt ccggcgtact gttttttgat
     8881 cgtcactacc gccagacgat aagaacagcc ttccggcggc aggtaaaaat cgacaatttc
     8941 cgggaactgt ttttgcagaa tcggtacaaa cacttcgttc aacgccacgc ccagtaccgc
     9001 gggctcatct ggcggacgcc cggtataggt ggaatggtaa atcgcatctt cacgctgggt
     9061 aatatgcgtc acggtaaata ccgggaaact atcgacttcg ttatagtaac cggtgtggtc
     9121 gccatacggc ccttccggcg cagtttcgcc ttgttcgata tacccttcca gcacaatctc
     9181 cgcactggcg ggcacttcaa ggtcattgga gatacacttc accacttcgg tcttggtacc
     9241 gcgcagcaat ccggcaaacg catactctga aagtgtatcc ggaacggggg tgactgcacc
     9301 gagaatcgtg gcgggatcgg cacccagcgc cacagaaacc gggaaacgtt cgcccggatg
     9361 cgccgcacac cactcctgat aatccagcgc gccgccgcga tgcgacagcc agcgcataat
     9421 cagtttgttt ttaccaatca gctgctggcg ataaatgccc agattctgcc gctctttatg
     9481 cgggccgcgc gtcactgtca gcccccaggt aatcagcggt gcggcatctt ccggccagca
     9541 ggtcataatg ggaatgcgat tgagatcgac gtcatcgcca gagacgattt tttgttggca
     9601 gggcacacca cgcagccgct ttgtcggcat gttcaatact tgcttaaact gcggcagttt
     9661 atcaaacagg tcgcggaaac cttttggcgg ctccggctct ttcagaaacg ccaataattt
     9721 accaacttca cgcagcgccg aaacatcttc ctgtcccatg cccatcgcca cgcgctttgg
     9781 cgtaccgaac aggttgcaca gcaccggcat tgagtagcct ttagggtttt cgaacaacag
     9841 cgcaggccca ccggcacgca aagtgcggtc agcaatttca gtgatttcca gatgcggatc
     9901 caccgggagc gtgatacgtt ttagctcacc ctgctgttca agcagcgtca agaagtcgcg
     9961 taaatcgtta tatttcatgg cgtccattgt agcctcttaa tctacgccca ttatacggcg
    10021 ttcatctttg cgatgctgta aatttgttaa attagcgtga actctgacgg tataacgcaa
    10081 accggggaat ataattaact tagcgtaaag cttttgctat ccttgcgccc cgattaaacg
    10141 gataagagtc attatgcaat cctggtattt actgtactgc aagcgcgggc aacttcaacg
    10201 tgcccaggaa cacctcgaaa gacaggctgt gaattgcctg gcaccgatga tcaccctgga
    10261 aaaaatcgtg cgtggaaaac gtactgcagt cagtgagcca ttgtttccca actacctgtt
    10321 tgtcgaattt gatccagaag tgattcatac cacgactatc aacgcgaccc gcggtgtcag
    10381 ccacttcgtg cgctttggcg cgtcgccagc gatagtccca tcggcggtga ttcatcagct
    10441 atcggtatat aaaccgaaag acattgtcga tccggcaaca ccttatccgg gtgataaggt
    10501 gattattacc gaaggcgcgt tcgaaggctt tcaggccatt ttcaccgaac ccgatggtga
    10561 ggctcgttcc atgctattgc ttaatcttat taataaagag attaagcaca gtgtgaagaa
    10621 caccgagttc cgcaaactct aaaacgcaat cccaaacagt gttttgacat tggcatccgt
    10681 ggtggcagcc agccatgcgg catcttctcc acgccagtgc gcaatacgtt gcaaaatatg
    10741 gtgcagatgg gctggctcgt tgcgccggga tgatggcttt ggcgtgaaat cgcgcgggag
    10801 cagatacggc gcatcagttt cgatcagcaa tttctccgcc ggaatcaacg gcaacaattc
    10861 ccgcagctcc agcccgcgtc gttcatcgca aacccaaccg gtaatgccga tataaattcc
    10921 acgcgccacg cacgcctgca tctcttcgcg tgtgccggta aagcaatgaa gaaccgcacc
    10981 aggcagttta tccagccacg gctccagcaa tgtcataaac cgctcgtggg catcgcgaca
    11041 gtgcataaat accggcatgt ttaattctgc ggcaatgcgt agctgagcaa caaaagcgcg
    11101 ttcctgctct tccggcgtcg aaaagttgcg gttaaagtcg agaccacatt caccaatcgc
    11161 caccacttct ggctgcgcgg ccagctcaat aatcgcttct tcagtcgcag cttgccactg
    11221 gctgctgtcg tgaggatgta cgcccgccgt tgaccaacag gacgaatact gacgcgccag
    11281 cttttgcgcc tgctggcttt cacgcaggtt agtgccggtg atgagtagcc cattaactcc
    11341 cgcgtcaaaa gcgcgcgcta caacatcatc acggtctttc gcaaattgcg aactggtcaa
    11401 attaacgccg atatcaaaca tcctgtactc catatgacaa ccgccctgac gggcggttga
    11461 atttattctt cagttttttc gctttctgct tcagcgtcgt tttcctcttc ccgatttcgc
    11521 cctttaccaa cgtaaaagcg tgagaagaag acaccgattt caaacagaca gtacatcggg
    11581 atcgccaata gcgtttgcga gaagacatcc ggcggcgtca gcaacatccc gacaacgaat
    11641 gcaccaacca gcacatacgg gcgttttttg cgtaagtctt ctggcgaggt aatccccatc
    11701 cagcacagca gcacaattgc caccggcact tcaaaggaga caccaaacgc cataaacagc
    11761 gccataacga agcttaaata gctggcgatg tcggtggata cctgcacccc ttccggcgcg
    11821 gtattggcaa ggaagccaaa tgccagcgga aagaccacaa agtaggcgaa tgccatgccg
    11881 atataaaaca gcagagagct ggaaaccagc agcggcacca ccaggcgacg ttcatgctta
    11941 tacagcgctg gggcgataaa tgcccacacc tgatagagaa tcaccggcgc tgacagaatc
    12001 agcgacacca taaaggtcag cttgatcggc gtaaagaacg gcgaggccac gtcggtggcg
    12061 atcatcgttg aaccttgcgg caactgcttg atcaatggcg cggataccag gtgatagatg
    12121 tcattggcga aatagaccag acacaggaat atcacgatca ccgcgataat gcagttcagc
    12181 agacgcttac gcagctcaat tagatgcgtg ataagcggtt gagtatcttc tacagacatg
    12241 tttacggttt atcactcgac gaaggggaag gtgcagcggt tttcggttca gcgtccgcag
    12301 caggttttac caccggctct ggcgtggttt ctggcttctg ttccggcgaa ctggcctgcg
    12361 tttgtgcagc ggcaggcgtt acgccctcat gcgcagcttc attatctttc accaccgggt
    12421 tatggatggt gtgcgcttca tcgctcgcct tttctggatc gtttgcaacg taggaacgct
    12481 tcatcgactc cgcggcctgg cgtagttcat ccatcgacgc tttcagttcg ggcgtcaggt
    12541 tagtgaggct cgccttttca acctttttca gactgtcctg aaactcctgg agttttaact
    12601 cctggatcag ttcgttctgc accgttgtcg ccagtgaacg caacgcgcga atccagcccg
    12661 ctaccgtttt taccgccaca ggcagtcgtt gcggccccag aacgacgagg ccgatgatga
    12721 acaccaatag cagttcgcta aaaccgatat caaacacgga ttacacctgc tctttatcgt
    12781 ggcgcttcgc gtcttctgtt ttagcctgtt cctgattcgt atccgcctgc ttatcggcga
    12841 tagttttcgc agtaaaatca gcatcctgac tggttttatc ctgctttggt tcatcatcgc
    12901 tcattgcttt tttaaagcct ttgatcgacg caccaagatc ggaaccgatg gagccgagct
    12961 ttttggtgcc aaaaagcagt acaacgatga cggcaataat caataactgc caaatactga
    13021 taccacccat acatgttcct ctgtggtaga tgatgattaa acaaagccgc attatacgtt
    13081 acacggcccg ccttgagcga tgaaaaaatc agcgtgtttt gcgccaaccg acaaaccagg
    13141 cgatcagacc acctgccatt aaccagccgg gcatcagccc ccactcaggt cggctgacca
    13201 acaagaatgt gccacttaat actaacgtag cgccaattcc gagaaaataa cgcgattgtc
    13261 cctgacgtac atgatttgac tgaagctcgc gggcaatctt atcaacactg tgctgtaaat
    13321 acttgccctg gcgcaaactg tcgtaaacca attcaggcag ttctggcatt ttttcgaccc
    13381 agaacggcgc tttttcttta aatgctctca ccagcgcagg aataccgacc tgatctttaa
    13441 tccacgactc caggaaaggc ttcgctgttt tccataaatc gagctgcgga taaagctggc
    13501 gtcctacccc ttcgacatag agcagggttt tctggagtaa caccagttgc ggctgcactt
    13561 ccatattgaa gcgacgcgcc gtattaaaca gatttaacag tacatgtcca aacgaaattt
    13621 cggccagcgg tttctcaaag ataggttcac agaccgtacg aatggcaaat tcgaactctt
    13681 caacgttggt atctggtggc acccagcctg aatcgacgtg tagctctgcc actttgcgat
    13741 agtcgcgatt aaagaaggcg ataaagtttt ctgccagata gcgtttatct tctttgttta
    13801 gcgagccaac aatcccgcaa tcaatgccga tatatttcgg gttttccggg tgttcatagc
    13861 ttacgaagat gttgccaggg tgcatatcgg catggaaaaa gctgtcgcga aagacctgag
    13921 tgaagaacac ctgcacgccg cgttccgcca gcaatttcat gttagtgccg tttttctcca
    13981 gcgccgcaac atcagacacc ggaatgccgt aaatgcgctc catcaccatc atcccttcac
    14041 tacaatagtc agggtaaact tccgggatgt agagcatcgg gctgtcttca aaattgcgcc
    14101 gaagctgaat ggcgttggca gattcccgca gcaaattcag ttcatcaatc aaggtctttt
    14161 cgtactcgcg caccacttcg gttgggcgca gacggcgacc atccggcagc aaacgcggca
    14221 cccagcgagc cagacggtag ataagtttca gatccgcttt aatgaccggc aaaatatccg
    14281 ggcggatgac tttaatcacc acctctttac cattcgattt caatcgcgcg gtatgaacct
    14341 gggcgataga agcagaagcc agcggcttga tttcaaaatc gtcaaaccac gcttctaccg
    14401 gcaagccgcc cattgcagct tcaatctgct gcttcgccag cttgccatca aacggagcta
    14461 ctttgtcctg caataacgcc agctgatcgg caatatgcgg tggaaaaaga tcgcggcggg
    14521 ttgataacat ttgcccgaac ttgatccaaa ccggccccag ttcttgcagg gccagtcgta
    14581 gtcgctcacc taaaagtttg tctttatgcc gatttggcat ccagaataat gagtatcgcc
    14641 atagccgtag cggcagggtg atacgcattt tggggatcag ttcatcaagt ccgtagctta
    14701 aaaaagtgcg aatgatgaaa tataggcgcc gtacttcacc tggcgtcatt tagcctccag
    14761 tttttccagc cgtttggtca gggcatcaac agcacgctcg acggcagccg tctcttccgc
    14821 aaaccaggcc acttcaagcg gaccgggtgc catacgccac tcttcagtaa tggcttccgc
    14881 cacataacgt tgctggcgct taatgccgtg atgcaggaac tttgcgcctc cgcgcatggc
    14941 tttgctgatt ccttcagcgg cgatatcacc ggtataaggg gccagcagtt ccgcagggtc
    15001 gaactctgcc agatctgcca gcgcaacgaa gttttgcacc acctgaatat cgccctgcac
    15061 ttccagctca ccactgcgaa tcagtgcggt aagctgctgg cgatcgcgaa gtttcggcaa
    15121 cacactggcg taggcgataa cggtgcagtc agcatcgcct gcccattcgc ccagtacatc
    15181 aacctggcgt tcgctgaaca ccagaatcaa tgacgtcgaa aagcctttta cctccacgcg
    15241 caatacttta cccagcagac gcgagcgggc tgttttcagc gcgggtgagc gatacaggaa
    15301 ggtgttgagc agactttcaa ttcctgccgt cactaaaggt ttaaaaggca tttccggtct
    15361 cctgtcagaa cttataacca cgatgcagcg ccacaacccc tgccgtcaga ttgtagtagt
    15421 cgacactttc gaatccggca tcctgcatca tggctttcag ggtatcctga tcgggatgca
    15481 tacggatgga ttctgccaga taacggtagc tgtcggcgtc gttcgcgacc agtgagccaa
    15541 tacgcggcag cacatggaag gagtatgcat cataggcttt gctcagcggc tcgataattg
    15601 gcttcgagaa ctcaagcacc agcaggcggc cgccgggttt cagcacgcga tacattgaac
    15661 gcagtgcttt atctttgtcg gtgacgttac gcagaccaaa cgaaatggtg atgcaatcaa
    15721 aggtgttatc cgggaacggc agcgcctcag cgttcgcctg aacatactca acgttgccaa
    15781 tcacaccgat attacgcagc ttctcgcgtc ccattttgag catggattca ttgatatcag
    15841 caaggaccac tttgccagtt tctccgacca ggcgggagaa tttcgctgtc aggtcgccgg
    15901 tgccaccagc cagatccagc acggtctgcc cacggcgtac gccgctgcaa tcaatcgtga
    15961 atcgcttcca caaacgatga ataccaaatg acatcaaatc attcatgaca tcgtattttg
    16021 atgccacgga atggaaaacg tgggcgacca tatccgcttt ttgttccttc gcgacggtct
    16081 gaaaaccaaa gtgcgtcgtt tcttgtgact tatccaccat ctcaatgcct gctcatcaaa
    16141 aaattgttcc agaagtgtaa cagattgggc gtcgatgccc tagatttcta cccggcttaa
    16201 ctacccctaa tgggctagcg cgactgctga ttatattcat catcgcgttg ataagcttca
    16261 tcattcggct gctccggaac cgaccgaagt cgatactctt catcctggct caccgcctgt
    16321 tcagccaaat ccggattaat ctcgcgttta atttctactc ctaaaccgcg aaacgcttct
    16381 gcctgcgcca gcacatttcc gcgacctgaa gagagttttt tcattgcctg gcggtagtta
    16441 tcctgcgctt tgtcgagact ttgaccaatc gcggacatgt catcgatgaa caaacgcatc
    16501 ttgtcgtaca gcttgctggc acgatcggcg atttgctggg cgttgcggct ttgatgctca
    16561 taacgccaca ggttggcaat agtacgcaac gccaccagca gcgtagtcgg gctaaccagc
    16621 atgatattgt ttttcaacgc ttcggtgatc agctccggct ggcggtcaag cgccagtaaa
    16681 aaagcgggtt caacgggaat aaacatcagc acgtaatcca gagttcgcag ccccggcagc
    16741 tgttgataat ctttgcgtcc cagcaaacgg atatggttac gcaccgacgc gatatgttcc
    16801 tgtagcgcgc tttcgcgggt gtagtcgtct tcggcgttaa aatagcgttc ataggcgacc
    16861 agcgtcattt tggcgtcgat caccacatct tttccctgcg gcagccgcac gatgacatcc
    16921 ggctgcatcc gcgagcgggc gtcattttcg atgctgacct gggtttcata ttcataccct
    16981 tcacgcagac cggaagcctc cagcacccgc gtcaatacta cctcgcccca gttgccctgg
    17041 gttttattgt cgcctttcag cgcgcgcgtc aggttgatcg cttcctgggc catttgcgcg
    17101 ttgagttgct ggagattgcg aatttcgtgg gtcagggtat ggcgttcttg tgcttcttta
    17161 ccgaagctgt cctgaacctg acggcggaaa ccgtccagtt gttcacgtag cggcgacaac
    17221 aggctgttca gactctgacg gttttgctca tcaacccggc gattgctgtg ctcaaaaata
    17281 cggttggcga ggttttcaaa ctgctcactg aggcgctgct cgctgttaat catctggcga
    17341 attttatcgt cagcatgttg ctgtgcggct tccatccgcg tggttacttc acgcagatcg
    17401 gcctccagag aggtgttaat actttgcagg ctgcgcactt cgttattgag taactcgcac
    17461 tctgcacgcc agtgctcgct ttgggtaatt tgttgttttg ccgcgcttaa ctccgcgacc
    17521 atctcttcac gttcagctaa ttgctcggct ttttgctgcg catgttgata actggcaaac
    17581 agccagccaa ttgccacacc caccaacgca ataactgcgt aaaccatgat tgagaaatcc
    17641 acaacgcctc ctgacgcaac aacttacccg cacaaaatag aattgtgctg tacaaacgtc
    17701 cagttggaaa ggcatttcct gcgaggcgct acgcaaaaat acaaaaggcc gaacgcgtcg
    17761 gccttcagac aggagaagag aattacagca gacgacgcgc cgcttccacc acgattttca
    17821 ccgcttggct ttcagtttgt ttcatcgtct cagcattcgg gatctcttgc tgggtgcggt
    17881 taacgataac acccgctacc ataccggcac gcaggccctg acttgcacac atggtcagca
    17941 gggttgcaga ttccatttca tagttcatta cgcccatcgc ctgccactct tccatagaac
    18001 ctttaaagtg acgaactacg cgaccagagt aagtatcgta acgttcctgg cctgggtaga
    18061 aggtatcaga agaagctgtc acgccaacgt gagttgtcgc gccaatggat ttcgcagctt
    18121 caaccagcgc agtcgtacat tcgaaatcag cgacagccgg gaattccagc ggtgcgaagt
    18181 gcaggctcgc gccatccaga cggacagacg ccgtggtaac caggacatca cccacattaa
    18241 tatgcggctg aatagcgccc gttgtaccga tacgcaggaa ggtgcgaatg cccagctgtg
    18301 ccagctcttc aacagcaata gaggtagacg ggccgccgat accggtagag cagacgataa
    18361 caggtttacc atccagctct gcacgccagg tggtgaattc gcggtgagat gccagcttaa
    18421 ccggcttatc catcagcgcg gcgatctttt ccacacgatc cgggtcgcca gggacgatgg
    18481 caagcgtagc cccttgtaaa tcgtttttag tgaggccgag atgaaaaaca tcagacttgg
    18541 acatatacaa ctcctctgtg aatcggttta gtcagaagaa gcaaaaagac actttaccga
    18601 agggtttaac attttttcgt gatactcatc accatgacgc aaatgcgttg cataaattta
    18661 cccaccaaaa gtgatgcaaa tcacataaat cactcttcat tctgcaggac tgcataaaag
    18721 atgagccgat tcaggcaatg tgatttgttg catgctgact atagttaaca ttgcgcaaca
    18781 tttttataag ggtacggaga ataccatggc aacaacacaa caatctggat ttgcacctgc
    18841 tgcatcgcct ctcgcttcga ccatcgttca gaccccggac gacgcgattg tggcgggctt
    18901 cacctctatc ccttcacaag gggataacat gcctgcttac catgccagac caaagcaaag
    18961 cgatggccca ctgccagtgg tcattgtagt gcaggaaatt tttggcatgc atgaacatat
    19021 ccgcgacatt tgtcgccgtc tggcgctgga ggggtatctg gctatcgcac ctgaacttta
    19081 cttccgcgaa ggcgatccga atgattttgc cgatatcccc acgttgctta gcggtctggt
    19141 agcaaaagtg cctgactcgc aggtgctggc cgatctcgat catgtcgcca gttgggcttc
    19201 ccgcaacggc ggcgatgttc atcgtttaat gatcaccgga ttctgctggg gtggacgtat
    19261 cacctggctg tatgccgcgc ataatccaca gctaaaagcc gcagtggcgt ggtacggcaa
    19321 actgacgggc gacaagtcgc taaattcacc gaaacaacct gttgatatcg caaccgatct
    19381 taacgcgccg gttctcggct tatatggtgg tcaggataac agcattccgc aggagagcgt
    19441 tgaaaccatg cgccaggcgc tgcgggctgc taatgcgaaa gcagagatta tcgtgtaccc
    19501 ggacgccggg catgcattca acgctgatta tcgcccgagc tatcatgccg aatctgcgaa
    19561 agatggctgg cagcgtatgc tggaatggtt taagcagtat ggtgggaagc ggagttagtt
    19621 tcctcttagt ctatatttga gatggtgagt gaacgagtgc gttttgctgc catctctgtc
    19681 ccccaaaaaa taatcagtat gaattgattg gagattacga ctccgctatt tgaccttaag
    19741 aaagtcggca agtcatagtc ataaatgagt ctcttcaatg gaagacacct tagagattta
    19801 ccatctactc ccattcacaa acaaacatta attattatca acacattcac attaattata
    19861 aatcatctca gaccttcctg ataaaaagat attaaagtga aacattttgt ttttcacatt
    19921 ttagttcatc acttttaggc gtaaataaag gtaaaaaaaa caacaatact atttctcttt
    19981 cacaaaagaa ttaacaaaag gttttttaag gatgagtgat attatttact tgaccataac
    20041 cggagaacaa caaggatgta tttcctctct ttgtggaacg agcgcatcaa taggtaatcg
    20101 ctggcagatt ggccatgaag atgaaatctt cgcgttctcg ctctctaata gtattacgaa
    20161 tgcaggcaaa gggtcacaat tgcatggcct tagtttctgt aaattcttat taacgctatt
    20221 aacaataacg agcagttgtt catggcgttt gacttctatc ggatcaatag atttggtcga
    20281 tgggagaaat attacaacat tcaactccgc ggcgcgctct tatcagcaat caatcacctg
    20341 tttactgaga acaatctgga tacagaagcc atcactgtca gttatgaata tatactctcc
    20401 aggcatctga ttgctaatac tgaatttagt tatttggcat ttcccgataa ctacaaccgt
    20461 ctgtttatac cacggccaaa aactaaagct ggtaatggtt caaaacactt aatagtaaag
    20521 cggttggcag actgcttgct gctggcggca tatacaatgg gaatatcgaa ggattcaggg
    20581 aaacagcaga caagttgggg ggagatgcac ctgctggtta cgatcaattt atggataaca
    20641 aagggttact catcgctggt gcttcaatag ttgctggagc agttctgggg agaatgagac
    20701 tccctgattt ggaatcactt gagcatttcg gagccagagg agcagtatca ggaa
//
LOCUS       DAJTSL010000061        20694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTSL010000061 DAJTSL010000000
VERSION     DAJTSL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.061"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..220)
                     /gene="hyfA"
                     /locus_tag="OO433_002038"
     CDS             complement(<1..220)
                     /gene="hyfA"
                     /locus_tag="OO433_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6050527.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDD"
     gene            complement(473..943)
                     /gene="bcp"
                     /locus_tag="OO433_002039"
     CDS             complement(473..943)
                     /gene="bcp"
                     /locus_tag="OO433_002039"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6050528.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(943..1515)
                     /locus_tag="OO433_002040"
     CDS             complement(943..1515)
                     /locus_tag="OO433_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6050529.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            1661..2539
                     /gene="dapA"
                     /locus_tag="OO433_002041"
     CDS             1661..2539
                     /gene="dapA"
                     /locus_tag="OO433_002041"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6050530.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            2556..3590
                     /gene="bamC"
                     /locus_tag="OO433_002042"
     CDS             2556..3590
                     /gene="bamC"
                     /locus_tag="OO433_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6050531.1"
                     /translation="MAYSVQKSRQAKVAGVSLILLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGVRTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSASDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            3803..4516
                     /gene="purC"
                     /locus_tag="OO433_002043"
     CDS             3803..4516
                     /gene="purC"
                     /locus_tag="OO433_002043"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6050532.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKRFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            4684..5547
                     /locus_tag="OO433_002044"
     CDS             4684..5547
                     /locus_tag="OO433_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6050533.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            5562..7577
                     /gene="tmcA"
                     /locus_tag="OO433_002045"
     CDS             5562..7577
                     /gene="tmcA"
                     /locus_tag="OO433_002045"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6050534.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALL
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            7651..8349
                     /gene="ypfH"
                     /locus_tag="OO433_002046"
     CDS             7651..8349
                     /gene="ypfH"
                     /locus_tag="OO433_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6050535.1"
                     /translation="MKHDHFVVQSPDKPVQQLLLLFLGVGDNSVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(8459..8659)
                     /locus_tag="OO433_002047"
     CDS             complement(8459..8659)
                     /locus_tag="OO433_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6050536.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(8687..9814)
                     /gene="dapE"
                     /locus_tag="OO433_002048"
     CDS             complement(8687..9814)
                     /gene="dapE"
                     /locus_tag="OO433_002048"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6050537.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(9818..10174)
                     /locus_tag="OO433_002049"
     CDS             complement(9818..10174)
                     /locus_tag="OO433_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6050538.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPSIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            10555..10614
                     /gene="ypfM"
                     /locus_tag="OO433_002050"
     CDS             10555..10614
                     /gene="ypfM"
                     /locus_tag="OO433_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6050539.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(10713..13825)
                     /gene="acrD"
                     /locus_tag="OO433_002051"
                     /pseudo
     CDS             complement(10713..13825)
                     /gene="acrD"
                     /locus_tag="OO433_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(13988..15688)
                     /gene="narQ"
                     /locus_tag="OO433_002052"
     CDS             complement(13988..15688)
                     /gene="narQ"
                     /locus_tag="OO433_002052"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR6050540.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQHLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            15894..17873
                     /gene="aegA"
                     /locus_tag="OO433_002053"
                     /pseudo
     CDS             15894..17873
                     /gene="aegA"
                     /locus_tag="OO433_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
     gene            17941..18516
                     /gene="nudK"
                     /locus_tag="OO433_002054"
     CDS             17941..18516
                     /gene="nudK"
                     /locus_tag="OO433_002054"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6050541.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            18642..19685
                     /locus_tag="OO433_002055"
     CDS             18642..19685
                     /locus_tag="OO433_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6050542.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKNPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(19795..20694)
                     /locus_tag="OO433_002056"
                     /pseudo
     CDS             complement(19795..20694)
                     /locus_tag="OO433_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
BASE COUNT         5001 a         5292 c         5539 g         4862 t
ORIGIN      
        1 catcatccct ctgggagatg gcattgaccg ggcagacctg caggcaaggg gcttcctcac
       61 agtgatgaca cacgacaggg gcagtgattg ttgacgtctt cgccagggcc aggcgcgggt
      121 gttgctgtaa accttgcgtt ttatgcacgt ccgaacaggc agcgagacag gtattacatc
      181 ctgtacacca cagtggttcg gccaccacaa agcggttcat gtcggctcct tgaaaatgat
      241 atgaaacatt gcatttcata ttgcataaag gatgccagtc tgattaattt aatatattca
      301 ataaattatg tattaacagg gttctgaacg gtacaaaaat gatggtcatt aacgttcagc
      361 aaaaaatgga ttcgacaccc gtgtcgcagg ggaaagcgag aatgtgatct tgatcacgag
      421 aagtgaggtg tgctgaccgc aagcgcagcc agcacggaat ggagcaaagt aatcaggcgt
      481 gttctttcag ccagttcagc acaacgtcgt ggtgattgct ggttttaaaa tcgtcaaaga
      541 catgttcgat tttgccatca gcgtcaatca ggaagctgat gcgatgaata ccatcgtagg
      601 ttttgcccat gaaggacttt tcaccccaga cgccgaattg ttcgcacacc tggtggtcct
      661 catcagacag gagcgtaaag ttaagcagct ctttttccgc aaaacgggag agtttttcgg
      721 gtttatcggt gctgataccc agcacatcaa cgcccgcttt tttcaactca tccatgttat
      781 cgcgtaagcc gcaggcctgt acggtacagc cgggggtcat ggctttcggg tagaaataaa
      841 ccagaacacg ctgtccctgg aagtcggtca aattaacttg ttctccgtct tgatccggca
      901 agctaaattt cggtgcgata tcaccggctt tcagtggatt cattacttaa ctccatcctg
      961 ttcatcatgt tgggaataat tgacgacgtt aatactgcct tgtgcattga gttctgtaca
     1021 tagggctttg aacgcttgct caatatttgc tgcgtccgca gatgcggggc tgtgggcggt
     1081 gatctgaata tgcaactgcg cagcccgttc attttcagca ggttgcgtgc gcgacaccag
     1141 ctccgcaatg ttcatatgat gcgcgtcgaa aagtgctgtg aagcgttcaa ttaaatgcgg
     1201 ggagtctgca acatcgacct gaacccagac agatgctggc attggcggac gcggacgcgc
     1261 cgtcgtgcgc ttcatcacga ttaaaagatc cagttcggca cctttcaacg gtaacgttga
     1321 ttcaatcaga gtaatggcat tccatgaacc ggaaagcagc atgataaacg tgaactcttc
     1381 tcccagcatc gccaggcgac tgtcttcaat attacagccg caactactga catgacgggt
     1441 gatggtgttc acaattccag ggcgatcggc acccaacgca gtgatcacca gataatgttg
     1501 cgatgacagt gtcaaactgg ttattccttt aaggggtgag ttgttcttaa ggaaagcata
     1561 aaaaaaacat gcatacaaca atcagaacgg ttctgtctgc ttgcttttaa tgccatacca
     1621 aacgtaccat tgagacactt gtttgcacag aggatggccc atgttcacgg gaagtattgt
     1681 cgcgattgtt actccgatgg atgaaaaagg taatgtctgt cgggctagct tgaaaaaact
     1741 gattgattat catgtcgcca gcggtacttc ggcgatcgtt tctgttggca ccactggcga
     1801 gtccgctacc ttaaatcatg acgaacatgc tgatgtggtg atgatgacgc tggagctggc
     1861 tgacgggcgc attccggtga ttgccgggac cggtgctaac gctactgcgg aagccattag
     1921 cctgacgcag cgcttcaatg acagcggtat cgtcggctgc ctgacggtaa ccccttacta
     1981 caatcgtccg tcgcaagaag gtttgtatca gcatttcaaa gccatcgctg aacatactga
     2041 cctgccgcaa attctgtata atgtgccgtc ccgtactggc tgcgatctgc tcccggaaac
     2101 ggtgggccgt ctggcgaaag taaaaaatat tatcggaatc aaagaggcaa cagggaactt
     2161 aacgcgtgta aaccagatca aagagctggt ttcagatgat tttgttctgc tgagcggcga
     2221 tgatgcgagc gcgctggact tcatgcaatt aggcggtcat ggggttattt ccgttacggc
     2281 taacgtcgca gcgcgtgata tggcccagat gtgcaaactg gcagcagaag ggcattttgc
     2341 cgaggcacgc gttattaatc agcgtctgat gccattacac aacaaactat ttgtcgaacc
     2401 caatccaatc ccggtgaaat gggcatgtaa ggaactgggt cttgtggcga ccgatacgct
     2461 gcgcctgcca atgacaccaa tcaccgacag tggtcgtgag acggtcagag cggcgcttaa
     2521 gcatgccggt ttgctgtaaa gtttagggag atttgatggc ttactctgtt caaaagtcgc
     2581 gccaggcaaa ggttgcgggt gtttcgctta ttttattact cgctgcctgt agttctgact
     2641 cacgctataa gcgtcaggtc agtggtgatg aagcctacct ggaagcggca ccgcttgcgg
     2701 agcttcatgc cccggctgga atgattttgc cggtgacctc cggtgattat gcaatcccgg
     2761 tgaccaacgg tagtggtgct gtcggtaagg cgctggacat tcgtccacca gcccagccgc
     2821 tggcactggt ttctggcgtg cgtacccagt tcacgggcga taccgcttca ttgctggtgg
     2881 aaaatggtcg tggcaatact ctgtggccgc aggtggttag cgtgctgcag gcgaaaaact
     2941 acaccatcac ccaacgtgat gatgctggtc agacactgac catcgattgg gtacaatgga
     3001 accgtctgga cgaagacgag cagtatcgtg gtcgttatca aatctctgtt aagccgcagg
     3061 gttatcagca ggcggttacg gttaaactgc tgaacctgga acaggcgggc aaaccggttg
     3121 cagacgcggc ttccatgcag cgttacagca cggagatgat gaacgttatt tccgccggtc
     3181 tggataaatc tgcctctgac gccgcgaacg ctgcgcaaaa tcgtgcctcc accactatgg
     3241 acgtacaaag tgcagctgat gacaccggtt taccgatgct ggtcgtacgc gggccgttca
     3301 atgtggtttg gcaacgtctg ccagcggcgc tggaaaaagt gggcatgaaa gtgaccgaca
     3361 gcacccgttc gcagggtaac atggccgtaa cttataagcc gctgtctgac agcgactggc
     3421 aggaactggg cgcgagcgat ccaggcctgg catccggtga ctataaactg caggttggcg
     3481 atttagataa ccgcagcagc ctacagttca tcgatccgaa aggtcatact ctgactcaga
     3541 gtcagaacga cgcgctggta gctgtcttcc aggctgcgtt tagcaagtaa aaatacaggg
     3601 ctggagtcat ccggcccttt tttctgatat gatacgcaaa cgtgtgcgtc ggcagaaaaa
     3661 tgcaatttta gcggtaattc gcacgaaatt tgtttgtcgg acgtaggtcg gataaggcgt
     3721 tcttgccgca tccgacaaaa catccggcac accagacagc aaaagatttt aaaacgttaa
     3781 ttcacaccca ggagtgataa agatgcaaaa gcaagctgag ttgtatcgtg gtaaagcgaa
     3841 aaccgtatac agcacggaaa acccggacct gttggtgctc gaattccgca atgatacgtc
     3901 agcaggggat ggcgcgcgca ttgagcagtt tgatcgcaaa ggtatggtga acaacaagtt
     3961 caactacttc attatgagca aactggctga agcgggtatc ccgactcaaa tggagcgtct
     4021 gctctccgat accgaatgtc tggtgaaaaa gctggatatg gtgccggttg agtgtgtcgt
     4081 gcgtaaccgt gctgctggct ctctggtgaa acgtcttgga atcgaagaag gtattgagct
     4141 gaacccgccg ctgttcgatc tgttcctgaa aaacgatgcc atgcacgatc cgatggtcaa
     4201 cgaatcttac tgcgaaacct ttggctgggt gagcaaagag aacctggcgc gtatgaaaga
     4261 gctgacctac aaagcgaacg acgtgctgaa aaaacggttc gatgatgctg gtctgattct
     4321 ggtcgacttc aagctggagt ttggtctgta caaaggtgaa gtggtactgg gcgatgagtt
     4381 ctccccggac ggcagccgcc tgtgggacaa agaaacactg gagaaaatgg acaaagaccg
     4441 tttccgccag agcctcggtg gcctgatcga agcctatgaa gccgtcgccc gccgcctggg
     4501 tgtacagctg gactgatttt tctgttcatc atcttgccgt gcagctggca cggcaagaca
     4561 accgctctcg gaatatatgc attgaacgct cattcctccg ccatttcatc ccgctttcct
     4621 gtggtaatct tcctctgaac cacctacgat catttctata atgaatatat tgttgaggta
     4681 tctatgcgtt ggcaagggcg acgtgaaagt gacaatgttg aagacaggcg caacagctcc
     4741 ggtggtccgt ctatgggcgg tcccggtttt cgcctgccaa gcggtaaagg cgggctgatt
     4801 ttactgatag tcgtgctggt tgcaggctac tatggtgttg atttaaccgg gttaatgact
     4861 ggacagccgg tttcccaaca acaatcaacg cggtccatta gccctaatga agacgaagct
     4921 gcaaaattca cctcggtgat tctggcaacc acggaagaca cctggggaca acagttcgag
     4981 aagatgggta agacctatca gcaaccgaag ctggtcatgt accgtggaat gacgcgcacc
     5041 ggctgcgggg cgggccagtc cattatgggg ccgttctatt gcccggcgga tggcacagtt
     5101 tatatcgatc tctccttcta tgatgacatg aaagacaaac ttggcgcgga tggcgatttt
     5161 gcccaggggt acgttatcgc ccatgaagtc ggtcatcatg tgcagaaact gttaggcatc
     5221 gagccgaaag ttcgtcaact gcaacaaaac gcgacgcagg cggaagtgaa ccgcttatct
     5281 gtgcgtatgg aactccaggc cgactgtttt gccggtgtct gggggcataa catgcagcag
     5341 caaggcgttc tggaaaccgg cgatctggaa gaggcgctga atgcggcgca ggccatcggc
     5401 gatgaccgtt tacaacagca aagtcagggg cgagtagtac cagacagttt cacacatggc
     5461 acttctcagc aacgctacag ctggtttaaa cgtggtttcg acagcggcga tccggcacaa
     5521 tgcaatactt ttggtaaaag catttaattt ccggggcagg gatggctgaa ctgactgcgc
     5581 tttacacatt aacagcgcaa atgaaacgtg aagggatccg ccgcttgctg gtgttgagcg
     5641 gggaagaggg ttggtgtttt gatcatgcgc ttaagttgcg tgatgcctta cttggcgact
     5701 ggctgtggat ttcgccgcag ccagatgctg aaaaccactg ttctccctcg gcgctacaaa
     5761 ctttacttgg gcgcgagttc cggcatgcgg tattcgacgc ccgccacggc tttgatgccg
     5821 ctgcctttgc ggcacttagc ggaacgttga aagcgggaag ctggctggtt ttgttactcc
     5881 ctgtatggga agagtgggaa aaccaacctg atgccgactc gctgcgctgg agtgattgcc
     5941 ctgaccctat tgcgacgccg cattttgtcc agcatctcaa acgcgtactt acggcggata
     6001 acgacgctat tctctggcgg caaaaccagc cgttctcgtt ggcgcatttt actccccgta
     6061 ctgactggca ccccgcgacc ggcgcaccac aaccagaaca acagcaactc ttacagcagc
     6121 tactgaccat gccgccgggc gtggcagcgg taacggctgc gcgtgggcgc ggtaagtcgg
     6181 cgctggcagg gcaactcatt tctcgtattg cgggcagtgc gattgtcacc gcgcccgcaa
     6241 aagcggcaac ggatgtactg gcacaatttg cgggcgagaa gtttcgcttt attgcgccgg
     6301 atgccttgtt agccagcgat gagcaagccg actggctggt ggtcgatgaa gccgcagcca
     6361 tacctgcgcc gttgttgcat caactggtat cgcgttttcc tcgaacgttg ttaactacta
     6421 cggtgcaggg ctacgaaggc accggacgtg gttttttgct gaaattttgc gctcgctttc
     6481 cgcatttaca ccgttttgaa ctgcaacagc cgatccgctg ggcacaggga tgcccgctgg
     6541 aaaaaatggt tagtgaggca ctggtttttg acgatgaaaa cttcacccat acaccacaag
     6601 gcaatatcgt catttccgca tttgaacaaa cgttatggcg aagcgagcca gaaacgccgt
     6661 taaaggttta tcagttattg tctggtgcgc actaccggac ttcgccgctg gatttacgcc
     6721 ggatgatgga tgcaccaggg caatattttt tacaggcggc tggcgaaaac gagattgccg
     6781 gagcgctgtg gctggtggat gaggggggat tatctcaaca actcagtcag gcggtatggg
     6841 caggttttcg tcgcccgcgg ggtaatctgg tggcccagtc gctggcggcg cacggtagca
     6901 atccactggc agcgacattg cgtggacggc gggtcagccg gatagcagtt catccggctc
     6961 gtcagcgcga aggcgttggg caacagctca ttgccagcgc tttgcaatat acgcaagacc
     7021 tcgactatct ttcggtgagt tttggttaca ccggggagtt atggcgtttc tggcaacgct
     7081 gcggttttgt gctggtacga atgggtaatc atcgtgaagc cagcagtggt tgctatacgg
     7141 cgatggcgct gttaccgatg agtgatgcgg gtaaacagct ggctgaacgt gagcattacc
     7201 gtttacgtcg cgatgcgcaa gctctcgcgc agtggaatgg cgaaacactt cctgttgatc
     7261 cactaaacga tatcgtgctt tctgacgacg actggcttga actggccggt tttgcttttg
     7321 ctcatcgtcc gctattaacg tcgttaggtt gcttattgcg tctgctacaa accagtgaac
     7381 tggcattacc ggcgctgcgt gggcgtttac agaaaaatgc cagtgatgcg cagttatgta
     7441 ccacacttaa acttgcaggc cgcaagatgt tactggtccg tcagcgggaa gaggccgcgc
     7501 atgcgctgtt cgcacttaat gatgttcgca ctgagcgtct gcgcgatcgc ataacgcaat
     7561 ggcaattttt tcactgactc cttcaatttt ctcccatggt cattgtgagc cagcggcgta
     7621 aaattaatga catcagttaa ggagttcgcc atgaaacatg accattttgt tgttcaaagc
     7681 ccggataaac ctgtgcaaca gttgttactg cttttcctcg gtgtcgggga taactcggtg
     7741 gcaatggggg aaataggcag ttggtttgca ccgctgtttc ctgatgcgct ggtggtgagt
     7801 gtcggtggcg cggagccgag cggtaatccg gcggggcgtc agtggttttc ggtgcagggt
     7861 attacggaag ataatcgcca ggcgcgtgtg gatgccatca tgccgacgtt tattgaaacg
     7921 gtacgctact ggcagaaaca gagtggagta ggggctaacg ccacggcgtt aatcggtttc
     7981 tctcaaggcg cgattatggc gctggagagt attaaagccg aaccgggcct tgcttcacgc
     8041 gtcattgctt ttaacggacg ctatgcgagc ctgccggaaa ccgcgtctac agcaacgaca
     8101 attcacctga ttcatggcgg tgaagatccg gttatcgatc tggcgcatgc ggttgcggcg
     8161 caggaagcgt taatcagtgc cggtggtgac gtgacgctgg atatcgtgga ggatttaggc
     8221 catgcgatcg acaatcgcag tatgcaattt gccctggatc acttgcgtta taccattccg
     8281 aaacattatt tcgacgaagc gctaagtggc ggtaagcctg gcgatgatga tgtgattgaa
     8341 atgatgtaaa agacagatta cctggcctga taagacgcgt gagcgtcgca tcaggcatcg
     8401 ttcaccattg ccggatgcgg cgttaacgcc ttatccggcc tacggttgag cactcagctt
     8461 acttcttcgg ttgatccttt ttcggccagt cgtcgtcatc gtcccactta tcgttgaaat
     8521 cacgatgcgg gggaagctcc ggcttgttgg cgagaaattt cttgtggtcg acgcgcttga
     8581 gatctttaat cacgttcagc aggacgccta ccaaaaagac aatcaccaga atccaccaat
     8641 atttagccag ccagtccatg cttatttcct cttgcagaac cactcatcag gcgacgagct
     8701 gttccatgat acgttgatac atacgggcaa gtagctgcag gtcggcagcg ttcacacatt
     8761 cattaatttt atgaatagtg gcattgaccg gcccgagttc caccacctgc gcccccatgc
     8821 gagcaataaa gcgcccgtcg gacgttccgc ctgtggtcag tagctgcggt ttaatttcat
     8881 tatagtgctc aaccgcgtta acgaccgcat ccaccagttt accgcgcgcg gtcaaaaatg
     8941 gctgcccgga aagccaccaa tccaccgtat agcgcagttg atgtttttca agcagggcaa
     9001 gcacctgcgc tttgatcatc tcatcagtca gttcggtgct gaagcggaag ttaaactgca
     9061 caaacagttc acccggaata acattgttac tgcccgttcc cgcctgaata ttagcaatct
     9121 gcatactggt cgccgggaaa aattcattgc cctgatccca ctcaatagcc actaattcat
     9181 taaggaaagg tgctgcgcga tgtactggat tgtcagccag atgcgggtag gccacatgcc
     9241 cctgaacgcc atgaatggta aggttgcagg ttaacgatcc gcgacgacca tttttcacca
     9301 catcaccaac cacttcgata ctcgacggtt cgccgaccag gcagtaatcg agacgctcat
     9361 tacgtgccat caacgcttcg acgactttta ccgtaccgtt gtgggcactg gcttcttcat
     9421 cagaggtgat cagaaatgcc agtcgccccg tatggttggg atgttgtgcg acaaagcgtt
     9481 ctgccgctac caccatcgcc gccagcgagc ctttcatatc tgccgcaccg cgcccgaata
     9541 acatgccatc acgaatggtg ggttcaaacg gcggattgat ccaacgatcg gcgtcgccag
     9601 gcggcaccac gtcggtatgc ccggcaaagg ctaacgtttc accttgccca cgccatgccc
     9661 aaaaattctg cgtatcggca aagtccatgc gttcaacggt aaaaccgatc gcctgcaaac
     9721 gttcaatcaa caaagcctgg catcctgcgt catcaggact cagggaaggg cggcgaataa
     9781 gctgttgtgt cagctcaata accgggcacg acatagacta cacctcatgg aaaaattgct
     9841 gataactgga atcactgaaa cccagcagca taggcttacc aggcgcgcag agcaatggac
     9901 gtttgataat tgaaggcatt tcagtcatta atgccgccgc agaggccgca tcggtgattt
     9961 tattgcgggt ggtttcgtcc agtttacgcc aggttgtacc acgggtgttc agtaacgctt
    10021 cccagcctaa ttcgttgata aaaccgttca ataattcgct gtccagccca tcgacgcggt
    10081 aatcatgaaa acgatagtcg atgttattgg cttccagcca acggcgagcc tttttaatgg
    10141 tgtcacaatt tttgatgccg taaagtgtaa ccataatgaa tcctttttcg atctatgaga
    10201 ataaatactc atttaagggt gaaaacattt tgcctgcaaa tgattatcag gaaatataac
    10261 attatcgaag tattcaaatt aacaactatt aaataatatt aaacattttt aacgtaaaca
    10321 ttaagaatat tcatcttgtt atctgtctgt attttaacca gaattaaatc agtattagat
    10381 gccgtatgaa tatctcgaaa tgttgctgtg tatcacatac aaattatcac cgcaggacca
    10441 tagtatttac ataaggtttg tcccccggag gtagtcatcg cagcaatcga gtaaattagc
    10501 ttcaccacgg tatattgttt ccgtagaata cccataatta taagagaggt tgttatgatt
    10561 gaacgtgaac tggggaactg gaaagacttt atcgaagtta tgcttcgtaa gtaattcctg
    10621 agacaaagta caaacagcaa gaacccgctg agcaggttct taatcggaaa gttgaaaaag
    10681 gcgacacatt agcatgtcgc cttttttatt gcttattccg ggcgcggctt cagcgggaag
    10741 cggcggcgca ccagtacaaa gaacagcggc acgaagtaaa tagccagaat agtggccgaa
    10801 atcatcccgc ccattacgcc agtacccacc gcatgctgac caccggaacc ggcacccgtg
    10861 ctggttgcca ttggcaatac gccgaagata aatgccagcg aggtcatcag aatcgggcgt
    10921 aaacgctgac ggcaggcgtg gagcgtcgct tcaaacaggt cgtggccttt ttggttcatc
    10981 tcgttagcaa actcgacgat caggatggcg tttttcgccg ataaaccaat gaccgttaac
    11041 aggcccacct ggaagtaaac gtcgttttcc agcccgcgca tccaggttgc cagcagcgcg
    11101 ccgataaccc ccagcggcac gaccagcatt accgagaacg gcaccgacca gctttcatac
    11161 aacgccgcca gacacaggaa taccaccagc aaggaaatgg cgtacagcgc cggagcctgc
    11221 gcgccggaaa gccgctcctg atacgacatc gccgtccact ccagaccaaa gccgtttggc
    11281 aactgcttca ctaacgattc cattatatcc atcgccgtac cggtactgac ccccggtgcg
    11341 gcttccccaa caatctcaac cgcagaataa ccgttatagc gttccagacg cggcgagcca
    11401 gtctcccagc gtgaggtcgc gaaagcagag aagggcacca tgccgccatc tttatttcgg
    11461 acataccaga gattaatgtc atctggcagc atgcgatacg gcgcagctgc ctgcacatag
    11521 actttcttca cacgaccgcg atccataaag tcattcacat agctcgaacc ccaggcggtt
    11581 tgcagtgtgt cgttaatatc gtcgatagca acgcccagcg cctgagcttt acgctggtcg
    11641 atatcaatct gcaactgcgg actgtcatcg aggccgttat ggcgcacacg ggttagctcc
    11701 gggttttccg ccgccagcgc cagcaactgg ttacgtgctg ccatcagcgc atcgtgacct
    11761 gctccagcgt ggtcctgcaa ctccatatca aaacccgcag aactaccaag accgctaatt
    11821 gctggcgggc tgctggcgat aacgcgagct tctttaatct tgttaaacgc ctttgttgca
    11881 cgctcgataa tggcaaacga ggtgccggtc ttactgtcgc gttcgctcca gtctttcagg
    11941 cggataaaca ttcgcgccac gttttgcccg ttacccccag ggccagaacc aacggtggca
    12001 aacaccgaca tgatgttgtc tttttcatgg gtgaagtaat atttctcgat ttgctcaacg
    12061 actttcaggg tctgttgttg ggttgaaccg ctgggcaatt gtaccgaggt agtaaacatg
    12121 ccacggtctt ccagcggtaa gaacgacgtc ggcaaacgca ggaacaggaa caccatgccg
    12181 ccaagcagca ggacataaat cacaatccag cgcaggctgc ggtggagaat tttcgccacc
    12241 cctttttcgt agcgttcggc gttgcggtta aacatctggt taaaccaggc aaaaaagcct
    12301 ttttgcccgt gatgctcacc ttttttcagt ggcttaagta gtgtggcaca cagagccgga
    12361 gtgaggatca tcgctaccag tactgacagc accatcgccg caacaatggt aatagagaac
    12421 tggcgataga tggcaccggt ggtgccgccg aagaaggcca ttggtacaaa taccgccgaa
    12481 agcaccatcg caatcccgac cagtgccccc tggatctgcc ccatcgattt acgtgtggct
    12541 tcgcgaggag cgagtccttc ctcactcata atacgttcga cgttttccac caccacgatt
    12601 gcgtcatcca ccagcagacc gatcgccagt accatcgcga acatggttaa ggtgttgacg
    12661 ctgtaaccga aggcgtaaag tacggagaag gttcccatca acaccaccgg cacggcgata
    12721 gtgggtatca gcgtggcgcg gaagttttgc aggaacagat acataacgag gaaaaccagg
    12781 gcgatagctt ccagcagcgt tttcaccacg tcttcaatgg aggctttaac aaacgaggtt
    12841 gtttcatacg ccaccttgta ttccagtccg tgcgggaagt actgcgccag ctcgtcgaga
    12901 cgattgagca ccagctccct gtcgccattt cgtttgcgcc ggaggccagt tttaccccca
    12961 gcccggaggc tggcttacca ttgaagcggc taagataatc gtatttctcc gcccccattt
    13021 cgacggtggc gacatcgccc agccttacct ctgagccgtc ttgattcacc cgcaaggtga
    13081 tatcgcggaa ctgttctggt gtttgcagca gtgactgggc gttaatggtg gcgttgagcg
    13141 cctgcttatc gacggaaggt gtaccaccaa gttgcccaac cgcaatctgc gcgttctgtg
    13201 actcaatggc atcagtgaca tctttagccg tcatctggaa actgttgagt ttcgccgggg
    13261 ccagccagat acgcatggaa tattgcgaac cataggcatc gatatccccg acgccattca
    13321 cgcggcttaa cgggtcctga atattactgg caacataatc ggcaatatcc tgtttatcca
    13381 tcgaaccatc ggtagagacg aaagcaatgg tcagaatatt ggtatcgccg gttttacgca
    13441 ccgtcacgcc ctgattttgc accgcctgcg gtaactttcg catggctgat tgcagctggt
    13501 tttgtacttg ctgcacggct tcgtccggat cggtgcctgc tttaaaactt aaagtgacag
    13561 atgcctgacc ggtgccactg ctctgagatg acatatacat gagattatcg aggccggtca
    13621 tattttgctc gataacctgg ggcacggtgt tttccagcgt ctgggccgat gcgccgggat
    13681 agttagcggt cactcgcaca ttcggtggcg cgagatcggg gtattgttca acgggcaatg
    13741 aaaaaatcgc cagggtccct gtcagacaca acaggattgc cagcacccag gcaaaaatgg
    13801 ggcgatcaat aaagaaattc gccattaaaa gaggacctcg tgtttctgcg tatcgttgta
    13861 ggcttgactt gcttcactgt agcggcaagg tacgagccaa tcgtggagaa aaaaggagtt
    13921 aatgtaaatt atcatgccct tttatactga accttaagtg caagtattct ttggtcagta
    13981 ggaggcatta catcaactga ctttcctcac cctctgcaga gcgaaagcta atactcacta
    14041 acgtgccgcc gccggaaggt tgtgaaaaag tcagcgtccc acccagccgt tccgcgcgtt
    14101 cgcgcatgat attcagacca taatgacctt cgggttcttt cggttcaccg ataccaatcc
    14161 cgttatcacg gatataaacc gtgtgattgc cgtccggcgc ggtgacgcaa ctgacggcga
    14221 tttcgctggc gttggcgtgc ttcatcgcat tcagcaccgc ttcgcgaata atttgcaaca
    14281 aatgcacctg catttgcgca tccagcgcaa gggttggcag acggcagtcg agggtcagtt
    14341 tggcgctggt ttgattttgt aacgtatcca gcatttccct caacgcggag gggagatccg
    14401 cctgctgcag cgtcaggcgg aaagtagtca acagctcgcg taactgccga taagcatcat
    14461 tcaacgcctg ggaaaaatcg gccatgatac tttgtgcggt ggcgttatct tccggtatcg
    14521 aacgcttcag taacgtcaac tggatacgta agtaagaaag tacctgagcc agcgagtcgt
    14581 gcaattcgcg ggcgatggtt gcacgttctt ccatcaacaa taaatgctga aaatgcttct
    14641 gcgcctgatt aaagtacaaa ccgcgtccca gcatcgacga aacgctgtta agcagcggtt
    14701 ctgatgatga aacgtgacta ttttgccagt gcagttcgcc gtaaaccgtc tcttgcattg
    14761 tcaccggtaa aatctgcatc ggcaattccg ggtttggttg cccttcgcta atccgccagt
    14821 tttcaccgac atttaactcc agatattcag ccgcttcatt gtcgcgaaca atctgcaaaa
    14881 tatggcggaa acaatgcaca tcaatctggc ttgtgttcag cgcctgcgaa cattgataca
    14941 acacctccag acgacgcttg gcctcgtgga gatcgcgggt cttttcttct actgacgcct
    15001 ccagcgaaag gtacaattta tgcagctcgc tcgacatctg gttaaaggtt tttgcaagca
    15061 gaccaagctc attcggcaga ctggtatcca gcggcggcga gtcgaactgc ccgtgttcaa
    15121 tacgttgact ggcggtaacc agctgattca gcggggcaac cacctgatgg cgtatacggc
    15181 gcaaagtaaa aaagaccagc gtgaaaatac cgataccgcc agccagggaa atcgccacca
    15241 ccagcagcat tttgcgttca gcgtagtgct gtaaagccag cacgaacagg tctatctgat
    15301 taacataatt attaatattg gcctgatacc acggcaaatc gcccttgctg agccgattat
    15361 tcatctccag ccagttggca ttcagatgcg catagcgagt ttttactgct tctggcacat
    15421 accagacgtt gagattggtt aataccggtg aatgcagtgc ctgctgaaac aactggcgat
    15481 gtgcattgag ttgtggactg ccactttgca agtcgtagcc caggcggtaa ctctgcatac
    15541 gcagcgatcc ggcaatattg atagcctcag cgtcgcgcaa actgctcgcc agagttagca
    15601 gagcgatacc cgtggaaaga atcgacagca gcacaatgta aaaaaaggcc cgggccagac
    15661 tggccgagac gggtcgttta acaatcacac gcgtcttctc cacaaaaaat cattaatgtt
    15721 ccagttcgct gaaacgcaat ttagtcagtg atgggattat ttgagctgcc acagtttata
    15781 gcgaaatagt tacttattct gggcacatga acattgccga ttcccctgta agtcgggtaa
    15841 taacaacact catataaaga ataaggtttt tacaaccaaa aaagaaggtc gttatgaatc
    15901 gttttattat ggccaacagt cagcaatgtc tgggttgtca tgcttgtgaa atcgcctgtg
    15961 tcatggctta caatgatgag caacatgtcc tgagccaaca ccattttcat ccccgaatta
    16021 cggttatcaa acatcagcag caacgtagtg cagtgacctg tcaccattgt gaagatgcgc
    16081 cctgcgcccg tagctgccct aatggcgcaa tcagccacgt tgatgacagc attcaggtca
    16141 atcagcaaaa gtgtattggc tgtaaatcct gcgtggtggc ctgtccgttt ggtacgatgc
    16201 aaatcgtcct gacacccgtc gcggcaggaa aagtaaaagc cacggcgcat aaatgcgacc
    16261 tttgtgcagg gcgcgaaaac ggtcctgcct gtgtcgagaa ttgcccggcg gacgcgctgc
    16321 aactggtcac tgacgtcgca ctctccggca tggcgaaatc ccgccgcttg cgcaccgcac
    16381 gtcaggagca tcaaccgtgg catgccagta ccgctgcaca agaaatgccg gtaatgagta
    16441 aagtcgaaca aatgcaggca acgcccgcgc gtggcgagcc ggataaactg gcgattgaag
    16501 cgcgcaaaac cggttttgat gaaatttatc tgccatttcg cgccgaccag gcacaacggg
    16561 aagcctcgcg ctgccttaag tgcggcgagc acagcgtttg tgaatggacc tgcccgctgc
    16621 ataaccatat tccgcagtgg attgaactgg tgaaagccgg aaacatcgac gccgccgtcg
    16681 agctttctca ccagaccaac accctgccgg aaattaccgg acgcgtttgt ccgcaagacc
    16741 gtttgtgtga aggtgcctgt actattcgcg atgagcacgg cgcggtaact atcggcaaca
    16801 ttgaacgcta tatttcagat caggcgttgg cgaaaggttg gcgtcctgac ttaagtcatg
    16861 tcaccaaagt ggacaagcgg gtggcgatta tcggggcagg tccggcaggg ctggcctgtg
    16921 cggatgttct gacccgcaat ggcgtggcgg tgacggtgta cgatcgccat ccagaaatcg
    16981 gtggcttgct cactttcggc attccttctt tcaaactgga taaatccctg ctggcacgcc
    17041 gtcgggaaat cttcagcgcg atggggattc acttcgaact caattgtgaa gtgggtaaag
    17101 atgtctcttt ggattcgctt ttggaacaat acgacgcggt cttcgttggc gtaggcactt
    17161 accgttccat gaaagcgggt ttacccaatg aagatgcgcc gggcgtttat gacgcgctgc
    17221 cgttcctcat cgccaacact aaacaggtga tggggctcga agagctaccg gaagagccgt
    17281 ttatcaatac cgccggactt aacgtcgtgg tactgggcgg cggcgacacc gcgatggact
    17341 gtgtgcgtac tgcgctgcgc cacggcgcga gtaacgtcac ctgcgcttat cgtcgtgatg
    17401 aagctaacat gccaggctcg aagaaagagg tgaagaacgc ccgcgaagag ggggccaact
    17461 tcgaatttaa cgtccagccg gtggcgcttg agctgaatga acaaggtcac gtttgcggga
    17521 ttcgtttcct gcgcacgcgt cttggcgagc cggatgccta ggggcgtcgg cgtccagtac
    17581 cggtggaagg cagtgaattt gtcatgccag ccgacgcggt gattatggcg tttggcttca
    17641 atccgcacgg gatgccgtgg ctggagtcgc acggtgtaac ggtagacaaa tggggccgca
    17701 tcatcgcgga tgtggaaagc cagtaccgtt accagaccac caatccgaaa atcttcgccg
    17761 gtggcgacgc cgtgcgtggt gcggatctgg tggttaccgc aatggcagaa ggacgtcatg
    17821 cggcacaggg gattattgac tggctggggg taaaatcagt caaatctcac tgatagcctg
    17881 cgcagacaaa cccgacttca cagcgtaaga taattgttca tttcgcgctg tggagtcggt
    17941 atgacgcaac aaatcaccct cattaaagac aaaattctct ccgataacta tttcaccctg
    18001 cacaacatta cctacgatct cacgcgcaaa gatggcgaag ttatccgcca taaacgtgaa
    18061 gtttacgatc gcggtaatgg tgcgacgatc ctcctgtaca acgcgaagaa aaagaccgtg
    18121 gttctgattc gtcagttccg tgtcgctacc tgggttaatg gcaatgaaag cgggcagctg
    18181 attgaaacct gcgccgggct gctggataac gacgaaccgg aagtgtgcat tcgtaaagaa
    18241 gcgattgaag agacgggcta tgaagtgggc gaagtgcgca aattatttga actgtatatg
    18301 tcgccaggcg gtgtgactga gctaatccac ttttttatcg ccgaatacag tgacaatcag
    18361 cgcgctaacg ccggtggcgg tgtcgaagat gaagatattg aagtgctcga gctgccgttc
    18421 agccaggcgc tggagatgat caaaaccggc gagatacgtg acggtaagac ggtgttattg
    18481 cttaactatt tgcaaacgtc acatttaatg gactgaaaaa taacaataat atttcgttgt
    18541 ttattattgg ctcaatccga caaatccgat tgagccgcgc tacctgcgca acgaagatac
    18601 gacttgtgct gtttgtttga acttctgggg tcgtaccgtc catgcgctat cgcattttcc
    18661 ttctcttttt tttcgctttg ttgccgacgt ctttggtgtg ggcggcacca gcgcaacggg
    18721 cgttttccga ctggcaggtc acctgtaata accaaaactt ctgcgtggcg cgtaatacgg
    18781 gcgatcataa tggactggtg atgaccctga gccgcagcgc cggggcgcat accgatgccg
    18841 ttttacgtat tgagcgcggc ggattgaagt cgccggaggc gtcagaaggg gagatagcgc
    18901 cacggctgct gttagatggc gagccgttgg cgttaagtgg tgacaagtgg cggatttcac
    18961 catggttatt agtaaccgat gatacggcaa ccatcaccgc gtttttgcag atgattcagg
    19021 aagggaaggc gatcacctta cgcgacggca atcagaccat ttcgctgagt ggcttaaaag
    19081 cggcgttgtt gtttattgat gctcagcaaa agcgcgttgg cagtgaaacc gcgtggatca
    19141 agaaagggga cgaaccgccg ctcagcgtac cgcccgcgcc tgcgctgaaa gaggtcgcgg
    19201 tggttaaccc aacgccgacg ccactctcac tcgaagaacg caacgatttg ctggattatg
    19261 gaaactggcg gatgaatggt ctgcgctgct cgcttgatcc attgcgtcgt gaggtgaatg
    19321 tcactgcgct gactgatgat aaagcgctga tgatgattag ctgtgaggca ggggcctata
    19381 acaccattga tttggcatgg attgtgtcgc gtaaaaatcc gctggcctcg cgcccggttc
    19441 ggttgcgttt gccgttcaac aacgggcagg agacgaatga actggaactg atgaacgcaa
    19501 catttgatga gaaatcgcgt gaactggtga ccttagcgaa agggcgcgga ttaagcgatt
    19561 gtggcattca ggcgcgctgg cgctttgacg gtcagcgttt tcgcctggtg cgttacgccg
    19621 cagaacccac ctgcgataac tggcatgggc cagacgcctg gcccacgttg tggatcaccc
    19681 gttaaacaaa atggcgggga catgtaggcc ggataagacg ctgtaggccg gataagacgc
    19741 gtcagagtcg catccggcct acagcgtccg gcaatcagca tccggcgatc accatcaggc
    19801 acctttcact cccagcacct tatgcgcttt tgccacaata ttctcggcgg taaagccaaa
    19861 gaacgggaac agcttatccg ccggagcaga ttccccataa cctgtcatcc caacaatcgc
    19921 ccctttcaaa ccaacatact tgtaccagta atcggcaata cctgcttcca ccgccacgcg
    19981 agccgcaacg ttagaaggca acaccgactc ccgatattcc tcatcctggg cgtcgaaaat
    20041 atcagtcgag ggcagggaaa ctacgcgtac attgcgacct tctcccgcta atttctctgc
    20101 cgcttgcagg gggatttcca tctctgaacc ggtggcaatc aaaataatat ctggcttacc
    20161 gccgctgtct ttcagcacat aaccgccacg ggcaatctct tttacctgat ccggcgtacg
    20221 ttccacttgg gccagattct gccttgagag gatcagtgcc gtcggtccgt tgtggcgctc
    20281 aaccgccagc ttccagccca ccgccgcttc cacctgatcg cacggtcgcc aggtgctgaa
    20341 atttggcgtt aagcgcaggc tggccagttg ctcaacagcc tggtgcgtcg gaccatcttc
    20401 gcccaggcca attgagtcgt gggtataaac cataatctgc cgcgctttca tcagtgccgc
    20461 catccgcgcg gcgttacggg cgtattcaac aaacatcagg aacgtggcgg tatacggcac
    20521 aaagccgccg tggtgcgcga tgccgttggc tatagcggtc atgccaaatt cacgcacccc
    20581 gtagtgaatg tagttgcccg ccggatcttc cttcagcgaa acagaacctt tccagatggt
    20641 caggttgctg gagccagatc cgccgaaccg ccgagcaact caggcagcat cggc
//
LOCUS       DAJTSL010000062        20658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTSL010000062 DAJTSL010000000
VERSION     DAJTSL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.062"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..876
                     /locus_tag="OO433_002057"
     CDS             <1..876
                     /locus_tag="OO433_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HCR6050543.1"
                     /translation="DQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQ
                     YGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKG
                     FSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTN
                     IINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAED
                     GGFTLEINGSEKELYLLSGQQFLKCPLSVEKKRASIRYSGDVTCSVVTYSQLPELKQP
                     KLRGNVQTAQREVAP"
     gene            873..1962
                     /locus_tag="OO433_002058"
                     /pseudo
     CDS             873..1962
                     /locus_tag="OO433_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
     gene            complement(2050..3348)
                     /gene="exuT"
                     /locus_tag="OO433_002059"
     CDS             complement(2050..3348)
                     /gene="exuT"
                     /locus_tag="OO433_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6050544.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSRGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFSLVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            3831..5243
                     /gene="uxaC"
                     /locus_tag="OO433_002060"
     CDS             3831..5243
                     /gene="uxaC"
                     /locus_tag="OO433_002060"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6050545.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5258..6745
                     /gene="uxaA"
                     /locus_tag="OO433_002061"
                     /pseudo
     CDS             5258..6745
                     /gene="uxaA"
                     /locus_tag="OO433_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
     gene            6828..7379
                     /locus_tag="OO433_002062"
     CDS             6828..7379
                     /locus_tag="OO433_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6050546.1"
                     /translation="MTAYWLAQGVGFIAFLIGITTFFNRDDRRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTRALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7384..8628)
                     /gene="sstT"
                     /locus_tag="OO433_002063"
     CDS             complement(7384..8628)
                     /gene="sstT"
                     /locus_tag="OO433_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6050547.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDLSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9244..10209)
                     /gene="alx"
                     /locus_tag="OO433_002064"
     CDS             complement(9244..10209)
                     /gene="alx"
                     /locus_tag="OO433_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6050548.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINARVNYRHDKQRGE"
     gene            complement(10491..11477)
                     /locus_tag="OO433_002065"
     CDS             complement(10491..11477)
                     /locus_tag="OO433_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6050549.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLLHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(11556..12239)
                     /locus_tag="OO433_002066"
     CDS             complement(11556..12239)
                     /locus_tag="OO433_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6050550.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHSQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12316..12819)
                     /locus_tag="OO433_002067"
     CDS             complement(12316..12819)
                     /locus_tag="OO433_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6050551.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12904..14040
                     /gene="rlmG"
                     /locus_tag="OO433_002068"
     CDS             12904..14040
                     /gene="rlmG"
                     /locus_tag="OO433_002068"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6050552.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14325..14639
                     /gene="higB"
                     /locus_tag="OO433_002069"
     CDS             14325..14639
                     /gene="higB"
                     /locus_tag="OO433_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6050553.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14636..15052
                     /gene="higA"
                     /locus_tag="OO433_002070"
     CDS             14636..15052
                     /gene="higA"
                     /locus_tag="OO433_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6050554.1"
                     /translation="MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(15097..17115)
                     /gene="fadH"
                     /locus_tag="OO433_002071"
     CDS             complement(15097..17115)
                     /gene="fadH"
                     /locus_tag="OO433_002071"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6050555.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLHTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSESGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELNARRAIAQGTRLALEI"
     gene            complement(17541..19892)
                     /gene="ygjK"
                     /locus_tag="OO433_002072"
                     /pseudo
     CDS             complement(17541..19892)
                     /gene="ygjK"
                     /locus_tag="OO433_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(19909..20658)
                     /gene="ygjJ"
                     /locus_tag="OO433_002073"
                     /pseudo
     CDS             complement(19909..20658)
                     /gene="ygjJ"
                     /locus_tag="OO433_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
BASE COUNT         5035 a         5417 c         5429 g         4777 t
ORIGIN      
        1 gatcaaacgt catggcagtt atcgcatacc ttttataatg attcattcag ccataaagaa
       61 cttggcgtaa ccgtcggagg cctgaacacc gataccataa acagcgcggt taacgggcgt
      121 tgggatgggc aatacggaaa tgtctacgct accgtatctg atagttatga ccgtaagaat
      181 catgatcatc tttcggcctt tacgggcact tatagctcta cgctggctgt cagtcgctat
      241 ggcgttaatt tgggagccag tggtacagac gatttgctgg gtgcggtatt ggtggatgtg
      301 aaaggcttct ctgaacagga tgaagagagt caggatctgc aactcgaagc gcgggtggca
      361 ggcagccgaa ctttgcagct tggtcaaagc gacagtgtgt tgttccctta tcctggattt
      421 cagtctggtt ttgttgaggt taacgacagt agccagggca atcagcaggg gacaacaaac
      481 atcattaacg gtgcagggaa tcgtgaatta atgttgttgc ctggcaagct gcgctatcgc
      541 gaagtgtctg ccagctttaa ctacaactat atcggtcgtt tattactgcc ggcagcggtg
      601 aaaaaattcc cgattgtggg gctgaacagc gccatgttac tggtagctga agatggcgga
      661 tttacacttg aaattaacgg cagcgaaaaa gagctctatc tgctttccgg gcagcaattc
      721 cttaagtgtc cactgagtgt tgaaaagaaa cgcgccagca ttcgttacag cggagatgtt
      781 acttgtagtg tggtgactta ttcacaatta ccggagttga aacagcctaa attacgtgga
      841 aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt gcggcgatat
      901 tgggcttgtt tggcacgctc actggcgttc aggcagctcc tgacgtgacc agtgaaatta
      961 cgtatgattt ggcatctggt agagcggatt attacttctg gaaggatgaa gcgtcggcag
     1021 gaaataatgg atatatgtgg tatgaatgtt cgtatcctga cctccaacaa acctgtacag
     1081 ctaatggaaa tatatcgaca gtacaaatct atttaactga acaacgcagt gggatgcgtt
     1141 ggccggtaaa actcaaagga tttaaaacag ccattgtaag tagcgatgaa gcgccgctag
     1201 gatgcaaggg ggggcaaagg gcttcagacg aatcttaagg attctaatag atcttcatgt
     1261 acagaagatg gtcaacatta ttatatatac gatacaaagt ttcttacgct ctaccttgag
     1321 cagacagaga tgaagaattt gccgattggt ggcgtctgga aggggaaagt taaattacat
     1381 tcgaacagcc cggcccagga ctatttcgca aatattaccc tgaatacgct cgaccccaac
     1441 catattgacg tgttcttccc ggagttcgcc cacgccacgc cgagggtgca gttagacttg
     1501 catccaacag gaagcgttaa cggcagcaac tacgcgcaag atctgaccat gttggacatg
     1561 tgcctgtacg atggttttaa cggtaatgcc atcagttatg aaatcatgct caaagatgaa
     1621 gggcgaccag ctgcagggcg cagagacggt gacttctcta tctatcgtca gggaggaacc
     1681 tctaccgacg agggagaacg cattgattac cgggtcaaaa tgtacaaccc ggaaaccggt
     1741 gggcaaattg atgtgcgcaa taatgaaaat atggtctgga acagcattaa cctgaaaagt
     1801 gtgcgtccgg tggtactgcc cggtattcgc tatgccgtga tgtgtgtgcc gacacccttg
     1861 gctctggcag tcgataaatt cagcgtgatg gataaacagg ccgggtacta catgggcaaa
     1921 ttgtcagtaa tctttacgcc ttccttgcca accatcaatt aaccctgcaa agcggacaaa
     1981 cgcttttttc tatataaacg acgaagccgc ctgataaggc ggcttcgtcg ttataactta
     2041 cgttaaacgt taatgttgcg gtgcgggatc gttatgtgat tcctgcgcta cctcaatcgc
     2101 cggtttgttc tgcaacacgg tccagataac cagcgcacct aacaggtcga acactgccag
     2161 tactgcgaac agcgggctga agccgatggt gtcagccagt gcaccgacaa ccagcgaaaa
     2221 cagcgtactt gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt
     2281 acgaccgaac acgtcagaag agagcgtaat cagcgcgcca gacagtgcct ggtgagcaaa
     2341 accaccgata cacagcagca taattgcgac atacgggttg gtgaacaggc cgatcatacc
     2401 cgggccaatc atcagcactg cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt
     2461 cacaccaaac caacgctgga acagcggcgg caggtaacca ccgaggatac aaccgaggtc
     2521 agcaaacagc atcggcatcc aggcgaacat cgcgatctct ttcaggttaa agccgtaaac
     2581 tttaaacatg aacagcggga tccacgcgtt aaaagtaccc caggctggtt ctgccaggaa
     2641 acgcggcagc gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt
     2701 cttcgccgtg ctcacctggt gctgggcttc ctgaccatta ataatatagt cgcgttcttc
     2761 atcggtcaga tgcttctggt cgcgcggatg tttatagaaa atcagccatg ccatcgccca
     2821 gataaagctc aatgcaccgg agatgataaa tgccatctgc cagctgtgca ttacgattgc
     2881 ccataccacc agcggcggcg caatcatcgc accaatcgaa gaacctacgt taaagtaacc
     2941 taccgcgatg gaacgctctt tcgccgggaa ccattcggag ctggctttca gacccgccgg
     3001 gatcatcgct gcttccgcgg caccgactgc accacgagca atcgccaggc cacccctgct
     3061 accagccagc gcggttgcac cacagaatac ggcccacagt acagcaaaca ttgcataacc
     3121 gattttcgta cccagcacat ccagtacata gcccgctacc ggttgcatga ccgtataagc
     3181 agcagaatag gctgcgatga tataggaata ctgttgggtg gagatgttta actcttccat
     3241 cagagttggc gcagctgccg ccacagtgtt acgcgtcagg taaccaagca cggtgccaag
     3301 cgtcaccagt gcgatcatat accaacgtaa ccctttaatt ttacgcatgt aaaacctcat
     3361 cgttttgtta tatccgcagg aagcgcggcc atcatcccgc cagaggctga agatgttagt
     3421 tacgaccggg taaccgaaaa aaaaccggca cgccccgaac gttgccatga aagtttcgtg
     3481 ctaattcggc ttccgtaccg gttaacccga ttcatctaac aaatgaatga atcggcaatt
     3541 tgcattccgt cgacttatga tttgcgacgg cagaaagatg acttgtcata caactttaaa
     3601 aggtgagagc catcataaat gtgcgaatat ttgtagggac attacctgac gacagcgagg
     3661 ccagtactgg cgcggcctgc agcgagattt accactttga gagtaatttt tttaactacg
     3721 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     3781 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     3841 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     3901 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     3961 actatcgttt taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg
     4021 ctatgcgtac caacggtgtg gccgagcgtc tgtgtaccgg tgatgcgtct gaccgtgaaa
     4081 aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga
     4141 cgcacctcga actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg
     4201 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg
     4261 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     4321 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct
     4381 ggcgtccgga caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc
     4441 tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca
     4501 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg
     4561 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg
     4621 gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg
     4681 gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata
     4741 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc
     4801 gtccgatggc agaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     4861 cgaaaaccat tctgtactgc ctgaacccgc gcgataacga agtgttgggc accatgatcg
     4921 gtaacttcca gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     4981 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtttgctga
     5041 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     5101 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     5161 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     5221 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     5281 gctggataac gtcgcggtcg ctttagcgga tttggctgaa ggcacagaag tcagtgtcga
     5341 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     5401 tatcgcaaaa ggggccaatg tcatcaaata tggcctgccg attggttatg cattggcgga
     5461 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     5521 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcctgaagt
     5581 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc
     5641 aaccgtgggc tgtgtcaacg gcatcgcgcg gcagatccag aaccgttttc tgaaagagac
     5701 caacaacgct gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     5761 gctgggcgac gatcacatta atacccgcac catgctgcaa aacatggtgc gccacccgaa
     5821 cgcaggcgca gtgctggtga ttggtctggg ctgtgaaaac aactaggttg ccgcattccg
     5881 tgaaacgctg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
     5941 tgatgagatc gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga
     6001 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
     6061 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
     6121 taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
     6181 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
     6241 taaacagtac tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa
     6301 agcgggcggt atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc
     6361 cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccag ggctgaactt
     6421 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
     6481 tatggtgctg ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa
     6541 aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggctcgact ttgacgcggg
     6601 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt
     6661 tgagtttgcc aacggtaaac aaacctgcaa cgagcgtaac gacttccgcg aactggcgat
     6721 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
     6781 ataatgtgaa gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt
     6841 ggctggccca gggcgtgggt ttcatcgcct ttctgattgg tatcacaaca tttttcaatc
     6901 gtgacgaccg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
     6961 tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat
     7021 tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
     7081 gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg
     7141 tcagtaccag ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt
     7201 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg
     7261 agggtagttt tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa
     7321 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa
     7381 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
     7441 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
     7501 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
     7561 acaggccagc gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca
     7621 cagagaagcc accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt
     7681 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
     7741 ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag
     7801 tgccatattc accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct
     7861 ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
     7921 caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg
     7981 cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa
     8041 aataccaatc ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacag
     8101 atcgttgacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat
     8161 tgcccacacc aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac
     8221 catgctcatt accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc
     8281 cgcgctactg gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag
     8341 agcagcagag aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg
     8401 cccgtgctgg tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac
     8461 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
     8521 tgagatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
     8581 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
     8641 tcattgtgtt gtcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa
     8701 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
     8761 tttaaagaga ggtcatattt tgcaacttaa ttgttacatt tgtttgacat aaacagaggg
     8821 aaggactggc tactaaggct gattagataa catatgggca aaactatttg gaatatgaga
     8881 acggttgcgc tcctttaatg tgctatgcca tataaagtca ctggcgcagg gagcgcgcag
     8941 ggggcagcca atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct
     9001 gcgcgattcc ctcagcttat cccttgcggc taccgggtcg ggcgcaaggg aacgtccctg
     9061 taagatgcgc cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg
     9121 cgattttgac gccaccaaca cccgtgcggt ctttttgtaa agagctacaa gccaattaca
     9181 taattaatag ctcacctttg tctgggatga gttatctcta ggaatggctt aatatacagc
     9241 gaatcattct ccccgctgct tatcatgccg ataattcacc ctggcgttga taataaacgt
     9301 catcaccaga atgccaaaca ccacgcccag cgagacggcg attggaatat ggtagaagtc
     9361 gacaatcagc atcttgatac cgataaacac caggatcacc gccaggccat atttgagcat
     9421 cgagaaacgc tctgctacgc ccgccagcag gaaatacatc gcacgcaggc cgaggatcgc
     9481 aaacaggttt gaggtcagca caatgaacgg gtcagtggtc acggcgaaga tagccggaat
     9541 gctatccacg gcgaaaatca cgtcgctcaa ttccaccaga atcagtacca gcatcagcgg
     9601 tgtggcgtac agcaatccat tcttacgcac aaagaaatgc tcgttgtcga tggtgtcggt
     9661 catgcgcaaa tgaccgcgta gccagcgcac cagcggcttg tcgccaatgc ctgattcatc
     9721 ttcatgggcg agcgccatct tcacgccggt aaacagcagg aaggcaccga agatatacag
     9781 gatccagtcg aactgcgaaa ttagccagct gccagtgaag atcatgatgg tacgcagaac
     9841 aatcgccccg agcacgccat acaccagcac gcggcgttgt aatgccgccg gaacagagaa
     9901 atagctgaac aacatcagcc agacaaagac gttatcgacc gccagcgatt tctcaatcag
     9961 ataacccgtg agaaaggcca gtgcctgtgg atcggcaacg gcgcgacctt cggtttgcac
    10021 cagataccac cagaaagcgg cgttaaacag taacgacagc gtcacccaaa ccagcgacca
    10081 ggccgcagcc tgtttcatgg tcatggcatg tgccccacga cgcccctgca acaacaggtc
    10141 gatagccagc ataatggcga caacaacagc gaatccgccc catagcaacg gcgtgccgac
    10201 agtattcata gaagttcctt acacataaaa aaaacggcca acgtcggaag acgtcagccg
    10261 ctgcttttta tgcatagacc tcgccttccg gcaaggtctc acttacaaca aaaaagaata
    10321 cgtctgcgta ttcctttcgg gttgcccggt gaccggatgt ggtttttcac acatcgtaat
    10381 gacgatcgac cggcaatgaa gttactcccc tttgcaggta acaaagtatg acagccattc
    10441 agccagtcaa tggtctgtta catacctttt acactgtttt actttggcaa ttatagtttt
    10501 acgctatctg ccggaaaaat caccccagtc tggcggcgga tctcagtcag cagtttggcg
    10561 gtgatgcgac tgaccgccag ccctggatga tccaccagat gctcatccac cagggtagca
    10621 aacagctctg cttcgtagag catggtatta atatgctgcg gctgggtgag atcctgcatc
    10681 tggctgccac gcggcacgaa gcacactttc tggcattcag acagtttttc aatcaccagc
    10741 gatcccgctt cgccctgaat ctcgctttcc aggacagaat cactgacctt tgagtgcagc
    10801 aacgtaacgc tgaaatcacc gtaatccatc accaccacgc cgtgggcatc tacgccgctt
    10861 gccaacaaac tggcggttgc ctgcacgctt ttcggttcac cgaataatgc caccgccgac
    10921 gccaggcagt aaaagccgat atccataatt gaaccgttag agaatgccgg attaaaggtg
    10981 ttgggattct caccatccag gtaacgttga taccgcgagg aatactggca atagttgaaa
    11041 aagactttac gcaatttgcc gactttcggc agcgcctggc gcagcaaatg aaagttcggc
    11101 aggctggcgg ttttaaatgc ctcaaacagc accacctgat tttcccgcgc acaggcaatg
    11161 gcggcatcca cttccgccag attcgacgcc agtggtttct cgcaaatcac atgaatttta
    11221 tggctaagga aaagttgtgt ctgggaaaag tgcagggcat tcgggctggc aatatacacc
    11281 gcgtcaatgg catcgctttc cgccatcgct tccagcgagg taaacagatg ctcgacagaa
    11341 aaatcattgg cgaagtgctg ggcctgttca aggctgcggg aatatacggc ggttaacttg
    11401 tatttaccgc tctcatgggc ggcctcgacg aactggcgag tgatccagtt cgtaccaatc
    11461 acagcgaaac gtatcataac gcgtggatac tccataaagg gattctgtcg tcaatgtagc
    11521 acgccttttt catcttcgac gtgcgcagat acgcattatt cttttgccgg acagccatgc
    11581 tcactaaacg gcccaatcat gaccgatggc ccgagatatt tcggctggct atggaggatt
    11641 ttggcgtcaa tcacggcgct gacacgagcg agtttctctc gcagttgagt ataaaaaccg
    11701 tgcttcatat tgaggtcagg cgcgttaaaa ccgatagcgt cgatgctgta ctgttttgcc
    11761 agccagatgg cgcgttggtt atgaaattct tgcgagataa tggtgatatg gttttcacca
    11821 aaaacctttt tggcacgcac taccgaatcc agcgttgaga atccggcata gtcgcagaag
    11881 atcactttcg ccggtacgcc tttagcgatc aaagccttct gcatacctga tgcttcatcg
    11941 taatttttac gtccgttatc gccacttacc agtagccatt tcacttttcc ggcgtggtac
    12001 aacgctgctg cggtatcaat acgtcgggta aagtagcgat tacccggcct ggcccccagc
    12061 aataagccca cgttacgcgc cggaactgcg ttgacatcgc tccaggtcag ctgcttgctg
    12121 gcgttgacca ttactcgatc ggcgataaaa atggtcgctc ctgccacaag taacagtaag
    12181 caggcaatct ttagagtccg gcgagaaaaa caaatacgga gaagaaggcg gggctgcatg
    12241 gtcgttatcc ttaacgaaag gggggaggtc gattttaggc gctaaagaaa gaaaagataa
    12301 cgcctgacat cattttcaga ttttgtcctg cccaagcgac aactgcgtta gccacagccg
    12361 ggtgtcgaac tccagctggt ggtactgcgg ctccatatgg cagcacaact ggtaaaacgc
    12421 tttgttatgc tctttctcct tcaggtgcgc cagttcgtgc accacgatca tacgtaaaaa
    12481 cggttccggc gcgttgcgaa acacagtagc gacgcggatc tctgcttttg ctttcagctt
    12541 gccgccctgc acacgagaaa cagcggtatg taacccgagc gcgtttttta gcacatggat
    12601 cttattgtca tacatcactt tattgatcgg cggggcattg cgcagaaact gattttttag
    12661 atcctgagta tattgccaga gggctttatc ggtggcgtag tcgtgtgttc ccggatagcg
    12721 ttttgccagc acatcgccca gacgctgttc gttaatcagc gttcgcacct gggaaagtag
    12781 ctgctcggga taaccctgaa gataagtaag attgctcata accgcccacg aaaaagagaa
    12841 aaagggtata ctcacgcacc cttttcaggg gcaacgtcga aattttatca ttcaggaggg
    12901 ccgatgagcc acttagacaa cggtttccgt tcactgacac tacaacgttt tccggcgacg
    12961 gatgacgtta acccgctaca ggcgtgggaa gcggcggatg aatatttgct gcaacagttg
    13021 gacgacacag aaatccgcgg cccggtgttg atcctgaatg atgcctttgg tgcgttaagc
    13081 tgcgcgctgg cggaacataa gccgtacagc attggcgact catacatcag tgaactggcg
    13141 acgcgcgaga atttacgcct caacgggatt gatgagtcga gcgtgaagtt tctcgacagc
    13201 accgccgact acccgcaaca gccgggtgtg gtgctgatca aagtgccgaa aacactggca
    13261 ttgctggaac aacaactgcg tgcgctgcgc aaagtggtca cgtcggatac acgtattatt
    13321 gccggtgcca aagcccgtga cattcacact tccacgctgg aactgttcga aaaagtgctc
    13381 ggcccgacca ccaccacgct ggcatggaaa aaagcgcgcc tgattaattg cacgttcaat
    13441 gaaccgccgc tggccgatgc gccgcagacc gttagctgga agctggaagg tactgactgg
    13501 actatccata accatgcgaa tgtcttctcc cgcaccgggc ttgatatcgg cgcgcgcttc
    13561 tttatgcaac atctgccaga gaatctcgaa ggggagattg tcgatctcgg ttgcggtaat
    13621 ggcgttattg gtctgacgct gcttgataaa aatccgcagg cgaaagtggt gtttgtcgat
    13681 gaatcgccga tggcggttgc ttccagccgt ttgaacgttg aaaccaacat gccagaggcg
    13741 ttggatcgca gcgagtttat gatcaacaac gcgctctccg gcgtggagcc tttccgcttt
    13801 aatgctgtgc tctgcaaccc gccgtttcac caacaacatg cgctgaccga taacgtcgcc
    13861 tgggagatgt tccaccatgc ccgccgctgc ctgaaaatca acggcgagct gtatattgtt
    13921 gccaaccgtc acctggatta cttccataaa ctgaagaaga ttttcggcaa ctgcaccacc
    13981 atcgccacga ataataaatt tgtggtgctg aaagcagtga agctggggcg tcgtcggtaa
    14041 gattgggagt tacctgccgg atgcggcgtt aacgccttat ccggcctacg ttcagcacgt
    14101 tactcccagg ccggataaga cgcggcaagc gtcgcatccg gcaatgtctg cccagttctg
    14161 gaatcgcgct tccataaaac aaaaaatccc tctcaccatc ctctacattc tcttgtttag
    14221 cgtttttcta cgtttattct tccgtcacac agataaattc atacgttgcc aatctgtcaa
    14281 cggatgttat catattgcca atttgtcaac ggacgtgatg aagaatgcac ctgataactc
    14341 aaaaagcatt gaaagatgct gcggaaaaat acccgcaaca taaaacggag ttggtggctc
    14401 tggggaacac gattgctaag ggatatttca aaaaacctga gtcattaaaa gcagtattcc
    14461 catctctgga taacttcaaa tatctggata agcattatgt tttcaatgtt gggggcaatg
    14521 aattacgtgt tgtagcaatg gtcttttttg aatcgcaaaa gtgctacata cgtgaagtta
    14581 tgacgcataa agaatacgat ttctttaccg ctgttcatcg tactaagggg aaaaaatgat
    14641 tgcgattgcc gacatcttgc aagcaggaga aaagctaact gcggtggtac cttttctggc
    14701 gggtattcag aacgaggaac aatacaccca ggcgctggaa ctggtagatc atctgctgct
    14761 caacgatcct gaaaacccct tgctggatct ggtgtgtgcc aaaataaccg cgtgggaaga
    14821 atcagcgccc gaatttgcgg aatttaatgc catggctcaa gccatgcctg gcggtatagc
    14881 cgtgattcgt acccttatgg atcaatatgg tttaaccctt gccgatctgc cggaaattgg
    14941 cagtaaatct atggtgtcac gcgttttgag cgggaagagg aaattaacgc tggaacacgc
    15001 taaaaaattg gcaacgcgat tcggcatttc tcccgccttg tttattgatt aacgcgacgt
    15061 gccggatgcg ttgcatccgg cgcttcaaat aacgatttaa atctccagcg ccagccgtgt
    15121 tccctgggca attgcccgtc gtgcgttcag ctccatagcc acatcgcagc cgccaattaa
    15181 atgcacagtt ttcccgctat caatcagcgg ttgcgccagc gcgcggtttg gctcttgccc
    15241 tgcgcagatc accacattgt ccacagctaa tacctgcgtt tcgccgttga tcaccacatg
    15301 cagcccgtca tcgtcaatct tctgataact tacgcctggg atcattttca caccccgcga
    15361 gagcagggtg gtgcgatgga tccagccggt ggttttgcct aacccctgtc ccggtttgct
    15421 ggctttgcgc tggagcatca caatttgccg tgggctacgc gggatctgca ttccctgcgg
    15481 gcttaagcca ccagcctgtt gtaggctgct gtcgatcccc cactcattac agaacctggc
    15541 gatattctgg ctggttgatt cgcccggctg acttaaatac atcgccgtat caaaaccaat
    15601 cccgccacaa ccgatgatgg caaccttgtt gcctaccggc gctttgtcgc gcagtacatc
    15661 gagataactc aataccttcg gatgatcgat cccgtcgata ggcggaacgc gcggcacgat
    15721 cccactggcg aggatcgttt catcgaacgc ctgtaactga tccgccgtca cggtgtgatt
    15781 gagtttcagc gtcacgcccg tcacttcgat catccggtgg taatagcgca gcgtttcgta
    15841 aaactcctct ttgccgggga tctgtttggc gatattaaac tgcccgccgc tctcgctgtg
    15901 ggcatcaaac aatgttacct gatgcccacg cgccgccgcg ttaatggcaa acgccagccc
    15961 agcaggtccc gcaccgacca ccgccagatt ttttttctgc acggcaggaa ggattggcat
    16021 tttggtttcg tggcaggcgc gaggattcac caggcacgag gtgactttgc caacgaagat
    16081 ctgatcgagg caggcctgat tgcagccaat gcaggtgttg atctcatcgg ctcgccccga
    16141 ttgcgctttt gacagcagtt ccgcatcagc aagaaacggt cgtgccatcg ataccatatc
    16201 ggcatcgccg cgcgagagaa tatcgtcggc aacctgcgga tcgttaatcc ggttggtggt
    16261 taccagcggc agcgagacgt ggcctttcag tttgcgcgtg acccagctaa atgcgccgcg
    16321 cggcacgggc gtggcaatgg tcggaatacg tgcttcatgc cagccaatgc cggtgttgat
    16381 aatggtcgcg cccgccgctt caatggcctg cgccagctct accgtttcgg caaaagtccc
    16441 gccgtcttcg accaggtcga gcatcgacag tcggtagata ataatgaagt cgttgccgac
    16501 gcgttcgcgc accgcacgca ctacttctac ggcaaatcgc atccggttgc ggtaatcgcc
    16561 gccccactgg tcactacgct gattggtgtg cagcgtcaga aattcgttga tcaaataccc
    16621 ttcggacccc attacctcta caccgtcgta tcctgcctcc cgtgccagtt gcgcgcagtg
    16681 ggcgaaatcg tcgatcagtt gcaggatctc ttcatgggtg agttcgtgag gaacgaaacg
    16741 gttgatgggg gcctgcaatg cggagggagc gaccagatgc ggttggtagc tgtagcgccc
    16801 ggtatgcaaa atttgcaggg ctattttgcc gccttcctga tgtaccgctt cggtaatggt
    16861 gcgatggtgt gggatctggc tggcgtcgtt gagcattgcg ccaccttcca tgccaacgcc
    16921 tgttaaatct ggtgcaatgc cgccgctgac aatcagcgcc acgccgtgac gggcgcgttc
    16981 ggcataaaac gctgccagcc gctcggcacc gtccgggtat tcctccagcc cggtgtgcat
    17041 tgagcccatc aacacgcggt tttttaacgt ggtaaaacct aaatccagcg gggcgaacag
    17101 cgacgggtag ctcataaaat tgtccagtat gtaaaataat tgttatgtgg tcggatgagt
    17161 tctaatttag ccgtcgccgg agaaaaggga aaaggggagt gcggggtttg tgatgggatt
    17221 caaaaagaat acgcccggtg gtaacgccgg atgcgacgct tgccgtgttt tatccggcct
    17281 gggggattgc acatgtaggg cggataaggc gttcacgccg catccgccag tggcgcggtg
    17341 cagatgccgg atgcgacgct tgtcgcgtct tatccgacct acgggaacgc acatgtaggg
    17401 cggataaggc gttcacgccg catccgccag tggcgcggtg cagttgccgg atgcaacgct
    17461 tgccgcgttt tatccggcca ggaggattgc acatgtaggg cggataaggc gtttacgccg
    17521 catccgccag cagttgcaaa ttattgctta cggaaaaaat cgttatacaa catatacaaa
    17581 tgcgcggcac tccaggagaa atttggtgcg ccttgctgcg cgccagtcag cgggttgtaa
    17641 ttctcctgaa ttgggccatc ggcggttaat cctttggcgt gccggaagaa cgtatccgcc
    17701 agcttcaggg catcatcacg ataaccgtaa cgctccatcc ctttcagagc aaaccagaac
    17761 tgatccaccc atacgcgccc gcgccagtag atatcagcgc caaaagccgg attggttaac
    17821 gccgccgttc ccagcgggac aaaggtgttg aactctttag gatcgagcat caccttcacc
    17881 accgcgtcgg cattggcctg cgttgccgca ccgttaaaca gcggcgacca gccttccggc
    17941 cctttaccgc gctcaacaat cggtttgccc gcgcagccgt tcgccagtgg tttatcttca
    18001 atacgtacgt catagtagaa ctgggtagtc gggtcgaaca tacaggtgtt gatgtagtcc
    18061 gcgagctgct gtgccaactg gcgatagcgt ttggcctctt ctggcttacc gaggatggtt
    18121 gccatctccg ccagataatg gttatcgctg tacatatagc tggcctgatc caccgactcc
    18181 tgcaatagcg agtagcccag caacgttccg tcctgactgc ggttttcggc gaatttcacc
    18241 gtccagtcgc tacgtttgcc accattagcg acatatttat ccaactgttc tttgtcgata
    18301 aaaccaaaga cggcggcatc atcacgcccc gattcccacg acgcagcgac ctgtgccgga
    18361 atttccagac tgtcgtactg gcctttctcc accacgcggg cgtagttgtt cagcccagac
    18421 tgcgtctctt ctttgttgcc tttcttcacc gcaaacagca tctcgccgct ctcagtgttg
    18481 tgggctttgt cgcgggtcgc gccatattcc ggcacgccgt tgccgttgtg atcgcggtta
    18541 cgtaaccacc agtcgtgata ggcgaccagt ttcgggtaca tctctgccaa ccaggcttta
    18601 tcctgggtga cgttgtacac ttctatcacc gaccaggcgg caaggctggg tttggtatta
    18661 cgttcgttcc agttgccgcc atcgccacca cgctcggggc taaggttcca cgctatcagg
    18721 tcggggacaa agcccacatc ctgcggacgc acgcggtcgc caggctggat ctgccaggag
    18781 aagaccgcgc ggatattctc tttggcgatg tccggattga aatgcgccat cgcaaacgcc
    18841 tgcttctagg tatcccacgg ccaggtctga ttgccggaga accagcgccc ggtcaccgac
    18901 ggtgtaacgg tgttaaattt caccgcaccg cccggtgagc gccagttacc gttgagcgtt
    18961 tcgatcgctt tcaccgcgac gcgcgtctgt tccggcgtcg catccggatt ggttaaccct
    19021 ttcttcaggt attcttccca gcgttgctgc gaggcggtga gataaaacgc cggacgcgcc
    19081 agaatatcgc ggatctgcat ttgctctttg ctaacttcct gagcggtcag cagatgggaa
    19141 taggtggtgt agagcgtggt cgaaccgttg atatgcgcct tactggtaaa gcgattaccg
    19201 ttgatttcag tctgcaccgg cagggattta tgcacctgat attccgattc gccggaggtc
    19261 agcagatccc aggtggcgcg cactttgcca aaggtgactt tcaggccatc acgggtggcg
    19321 ctgattttgc gctgatagtc agggtattcg ccagcaatgg ttttatcgga aagcggtttc
    19381 ccttctttcg cttccagttt ttccagcagt tcgccgtccc acaccagatc cagcggttta
    19441 ttgctggtga ttttggtttc cagtagtgac gtgcgcggcg tggcgaagcg cagagtcatt
    19501 tcgacttgca catcttttgc tgtcagtttt tgcaccagcg caccgggaat actgtatgcc
    19561 tccagcgtaa agtcgacttt cttgccgtcc tgccagacgg tcaggcggtc gaaattgctg
    19621 gccataaagt tgatgtactc ttccgtcagc agcgcaacgc ccggaaagcc gcccatggtg
    19681 ttagggccgt ctggcaacag atgaccatgc caggctccga gatcgaaaaa cggattaaaa
    19741 cgctggtgat cgtcgtaatc gtaatccttc atgtactgcg gcgcgccagt acggttaatc
    19801 acgtttttat aattgtcggc gttagcggca tgggcggaaa aacttatcag cagagcgcag
    19861 gttactggcg ttaaaatagt ttttattttc atgaaattac agtccttatt accagtagaa
    19921 atattgcatc aggaagattt ggtcgctggg atcgtgagag gcatttttca tgtagaagcg
    19981 gctatcaaaa gccgtggcga aacgtccgcc gccgtattcg taccagatac caaactgcat
    20041 gaaagaggtg gtttcatcgt cagcgtggtc agggcgatgt ttggcgtagg tgtaagcggt
    20101 ggagagataa acgcccttta acgcttcata catggcgctc accatcatcc cactttcact
    20161 tcccggtacg tccagcccgt cggcgcgtcc ggtatggtgc caggcgcgag cggcaaagtt
    20221 aggagttagc aggcccgtga gatagagctg tcctgtgccg tcggtgattt ccagaccgtt
    20281 catccaggtg atgccttcgg caaggtcata ctggatgtaa ccgttcatca ttgccggata
    20341 ggtgtatttg tcgtcatagc gatcgtattt gccaaaatgt agccaggctt tgctttcatc
    20401 atgatgtccg gcaggagtcg cggtgacgct ataacgaaag ttgccgctta agttctgtac
    20461 cttcagcgca gccataatgt cgcgggtatt gggaatgacg tagcccagat cgggggtgaa
    20521 atcgccccac cattgcaggt catccagcga ggaatcctaa cgcagactga gcatgatttc
    20581 ggtgccatca ttggtacgat aaccgccata gaaacggtta attccgccct cgaaaccacc
    20641 ccagctatgg tctggcac
//
LOCUS       DAJTSL010000063        20649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTSL010000063 DAJTSL010000000
VERSION     DAJTSL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.063"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..4461)
                     /gene="mukB"
                     /locus_tag="OO433_002074"
     CDS             complement(1..4461)
                     /gene="mukB"
                     /locus_tag="OO433_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6050556.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLDGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVVFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVCADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAAYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLVISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS"
     gene            complement(4461..5165)
                     /gene="mukE"
                     /locus_tag="OO433_002075"
     CDS             complement(4461..5165)
                     /gene="mukE"
                     /locus_tag="OO433_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6050557.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5146..6468)
                     /gene="mukF"
                     /locus_tag="OO433_002076"
     CDS             complement(5146..6468)
                     /gene="mukF"
                     /locus_tag="OO433_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6050558.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLQVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6465..7250)
                     /gene="cmoM"
                     /locus_tag="OO433_002077"
     CDS             complement(6465..7250)
                     /gene="cmoM"
                     /locus_tag="OO433_002077"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6050559.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7386..8165
                     /gene="elyC"
                     /locus_tag="OO433_002078"
     CDS             7386..8165
                     /gene="elyC"
                     /locus_tag="OO433_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6050560.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8142..9035)
                     /locus_tag="OO433_002079"
     CDS             complement(8142..9035)
                     /locus_tag="OO433_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6050561.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQEHFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9189..9935)
                     /gene="kdsB"
                     /locus_tag="OO433_002080"
     CDS             complement(9189..9935)
                     /gene="kdsB"
                     /locus_tag="OO433_002080"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6050562.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9932..10114)
                     /gene="ycaR"
                     /locus_tag="OO433_002081"
     CDS             complement(9932..10114)
                     /gene="ycaR"
                     /locus_tag="OO433_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6050563.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10166..11398)
                     /locus_tag="OO433_002082"
     CDS             complement(10166..11398)
                     /locus_tag="OO433_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6050564.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLALEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11435..12421)
                     /gene="lpxK"
                     /locus_tag="OO433_002083"
     CDS             complement(11435..12421)
                     /gene="lpxK"
                     /locus_tag="OO433_002083"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6050565.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGSKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12418..14166)
                     /gene="msbA"
                     /locus_tag="OO433_002084"
     CDS             complement(12418..14166)
                     /gene="msbA"
                     /locus_tag="OO433_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6050566.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14203..16475)
                     /locus_tag="OO433_002085"
                     /pseudo
     CDS             complement(14203..16475)
                     /locus_tag="OO433_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16682..16966)
                     /gene="ihfB"
                     /locus_tag="OO433_002086"
     CDS             complement(16682..16966)
                     /gene="ihfB"
                     /locus_tag="OO433_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6050567.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17126..18799)
                     /gene="rpsA"
                     /locus_tag="OO433_002087"
     CDS             complement(17126..18799)
                     /gene="rpsA"
                     /locus_tag="OO433_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6050568.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18910..19593)
                     /gene="cmk"
                     /locus_tag="OO433_002088"
     CDS             complement(18910..19593)
                     /gene="cmk"
                     /locus_tag="OO433_002088"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6050569.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VATFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19766..20529)
                     /gene="ycaL"
                     /locus_tag="OO433_002089"
                     /pseudo
     CDS             complement(19766..20529)
                     /gene="ycaL"
                     /locus_tag="OO433_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
BASE COUNT         4970 a         5713 c         5017 g         4949 t
ORIGIN      
        1 ttaactcgcc tgagaaggcg cttcgtcagt tcctggaagc gtttcaggga gttgcggcgc
       61 aaatcctcgc aggccgacga catgaacatg ttcggtattc tggaagactt tacgcaccag
      121 tttataggtg gtgcctttct ccgggctgat attttccggc gctgcgatga tgagctgcat
      181 ttgcaaacgc tcacacaatt caaacagcgt ggcgatagaa cgagcatcca gtcgcgctgc
      241 ttcatcgagg aacagcagac ggcaaggaga gatatcttta ccgcgcaggc ggcgagattc
      301 atcttcccag ctttgtacca ccatcaccag aatcgacata ccggtaccaa tcgcctcacc
      361 ggtcgacaat gcaccagact ctgcgcgcaa ccaaccatcg gaaccacggt taacctcaac
      421 ttccatttcc agatagttgc ggtaatccag cagttcttcg ccaatggtct gcggcgtgcg
      481 ctgccccata tcaatctgcg gattaagacg ctgatacaat ttcgccaacg cttccgagaa
      541 ggtcagacgg ttgctgttaa acagatcctg atgctgctcg tgctgttcag agagcacatc
      601 cagcagcatg gcgtgcgttt cacgcacgtt cacgttgagg cgcacgctgt tcacctgacc
      661 aaacgatacg ttctgcaacc cctggttgag catacggata cggttctgct cgcgctgaat
      721 ggttttgcga atgatgttcg ccacgctgcg ggaactgatc accagtttct gttcacggga
      781 ggttaattct tcggtcagac ggctaagttc gatctccatc tgttcgatag cttccaccgg
      841 atcatcggta cgaataatat cctgacgaat acgttcacgc agatgctgat aagccgccac
      901 gaagaactga attttacgct ccggacgttt cggatcttcc gacatgcgca gcacgtcgcg
      961 cagatgttcg ttatccgcca ccgccagacg cagcgcaccc aatgccttat ccgacataga
     1021 gcgcaaatca tcagcggaga gataagccag ctcacgacgg tgtaagcggc gctcaacgcc
     1081 gttatctttc accatgcgca tcaccgcaca ccagcccgct ttggcggtca ctacctgctc
     1141 gcgcatctca aagtaatccc gctccagctt gcgcagtttg cgggtcaggt tgtccatctc
     1201 cgcttcgcag aaggtaagcg ctttttccag ttgattgcgg cgtgaacggt tattgctcag
     1261 ttgcgcatgc agctcgtcac ggcgaatacg cgcccgctct tctgccccgc tatcagcaca
     1321 cacgccgata tcctgcaatt cacgttgcag atcgttgagt agctcttttt tggtgtcgta
     1381 agaacttttc agcgaagcca gcacctggtt gtactgactc aactgcgctg cgtgaccgcg
     1441 caacgcttcg cgagcgcggg tacgctccgc ttccgcctgt tccagacgtt cacgcagttt
     1501 ttcgttgaga tcgctgttac cgctaagcat ttctgccgag tcagagtagc taaagtgcgc
     1561 acgacgctgc accacttccg tcagggcaaa cgcctgctga cgggcatcgc gctgcatctg
     1621 ttgagagtac gcgtaatctt cttttaactg ttcgaactgt tccgggtcgc tctgcaatac
     1681 cgaaacgatc ggttccagtt tcgccagttg attgccaaac tgctgaacaa aacgcgcggc
     1741 ttcctgggct tcatccagac gttcgcggat ttcatcgacg cgatccgcca ggctatcatc
     1801 agccaacagg ttgagacgcg gcagaatgcg gttcagcgcc gtaacgccct ctttcgcctg
     1861 ctcaaactga atacgctgct gctggttatc attttcatga ttacttaacg cccgctccag
     1921 ttcgacgcga cggctgttca gttgacggat ttctgcttcc gggtcagact caaacacaac
     1981 cgccagatga ctgccgataa agcggctgaa cgcctgatgc agacgctgag ttttctgtac
     2041 atcaaaggag agcgtggcga agcgttcgga aagcacttca cgctcggcat ggaggctttc
     2101 aatacggctt tcacgcgcag cacgaccaaa cagcggcact tccgggaaac gtgaataacg
     2161 ccactgacga tcggcgattt tcaccactac cgctttttcc agctcatcaa cgctgaacac
     2221 gctgtcatcg aatgactgcg gatctccttc gatcagatag agatcttccg ggcaatcggt
     2281 caagccatct aggtgttcag ttacctgtga cagatctggc accacgatgg cgtggcgtga
     2341 cgggccatac agcgctgaga agtacggcgc atcttccagg ctaacgtcgt cataaatttc
     2401 tgacagcagc acaccaccaa aacgctccgc cagcgcgttc agacgctgat cttcagagcc
     2461 gccaggctgg cttaaacgtt cgatctcttc atcgacggcg tttttgcgcg cgcccacttc
     2521 atcgcgttca acaatcgcct ctcgctcacg ctccagcaac tgttgcagat attcggtgac
     2581 gtcctggctg gaggtaaact cttcgccgca ctgttcgctc aactggttga gactgttttg
     2641 cgctgccagc caaaccggcg cacgctgcat caaactctga atgcgagact gcagctgttc
     2701 ctgctcctgg cgcagtgcca tgcgctcttc acgggcgtta gacacgctat cggaaagaga
     2761 ggcaatgcgt gcttccagtt cctgatgcag ggcttccagt tcgtcgatat caaaattctt
     2821 gccctgacgt ttgcagaaat ctgccagcag acgctcagct tcttgctgct cgcgtagacg
     2881 ctgttccagt tcgcttaatc gcatccgcag cggctgaacc tgctctgcca ggtgacgctg
     2941 atcgacccct tcgcgcaata gttcgcgagc gacatcccac gcctcgttac gcgccagtgg
     3001 gccgttgatt gccaccacca gctgataagc ctgctcaaac tggctgtgcg cggtttgcgc
     3061 catgctcatt ttctgctcaa gagagagcat tttttcagtc gcttccagct ctttcgcctg
     3121 gaaggtttcc agccattcgg cggcgctgtc ggcggttaag tccggcagat ggcacagttc
     3181 tttggcacga ttaagcgcag caatcgcctg gttatactgg atcgcgcgcg tttgctggac
     3241 gtccagcgcc tgctggtagt cagcaagctg gcttttcagc tcgtccactt ccagttcggc
     3301 agcttccgcg cgagcctcgt tctcttcctg gcgttcgatg gcttctgcca ccacttcgtt
     3361 ttgctcttcc agacggatct gcagctcatc gagatccgct tcgtagcgtt cgatcttctc
     3421 ctgctgacgc agtgcggttt gcaccaggtt caggtgatcg ctggccgcct gataatccgc
     3481 ttccagatcg ccttcggcac cgttgtgctc tgccagctca cgcgccatat cgacgtgttt
     3541 gtactgctca gccgccagtt gctgacgcga agtatgtaac tcgcgacgaa actccagggc
     3601 tttgtcgaga tggacacggc gctcgttggc gtgacgcatg tagtccgccg ccacgtagtt
     3661 ggtagcttcg ctgatcagat gcttaaacag gtcacggtcc gactgggtga cacgaatcgc
     3721 ttccagcgtc atgcggtttt cacgcagcgc cgcttccatg tcctggaacg ctttacgcac
     3781 gccgctgttt tccggcaaca gatagtcgcg cagagaacgg gtaatggcac tggagatccc
     3841 gccatacagc gaagcttcga tcagacgata gaatttgcta cggtcagatg cggagcgcag
     3901 acgacgcgcg atgatgccca gatcgaacat cagcgagtgg taatcggtaa tggagttgaa
     3961 ctgtttaaac tgcacgcctt ccatcgcctc gagcttgtct ttcagctcgt taagcggcag
     4021 cacgcgcgcc tggcgttcgt tcagggtttc ggtcaccagc tgtgtcggct gcaccgacat
     4081 cggcagtccc tgaatggcaa atggtttgat atccacttta cgatcgcgtc cggcaacctg
     4141 ttgcagacgc acaccgacca ccacgcgctg gtggcgcgag ttaatggtgt cgagcatcga
     4201 ataacagaca cccgctttca gcttaccgtg cagaccttta tcgcgcgaac cgctggtggc
     4261 cccggcttcc gtagtgttac ggaaatgcag cagggtcagg tcggggatca gcgccgtaac
     4321 gaacgccgcc atggtggtgg atttacccgc cccgttaccg ccagaaagcg tcgtgaccag
     4381 ctcgtcaagg tcaaaagttc gggcaaaaaa gccgttccag ttaatcagcg tcagtgagcg
     4441 aaatttaccg cgttcaatca ttattcttcc tctccgctat ctggctgatt ctcttcggtt
     4501 tcatcgttga gttgcagatg attttcaatc ggcattgctt cgccatcacg aatcaggcga
     4561 cgctgtgctt cacggggatc gtcgccagca cgcacatcgg ctccgaagcg gaacaccgat
     4621 tcggtaatgc gaaacttgct gctgtcgtgg cccataaacc acaccatgcc taaacgacgc
     4681 agacggttga gcgaagaacg tactttctcc tgcaacttct gacggtcaac gtctgaaccg
     4741 gttgaacggt tgttcaccag tttcagcagt tttgcttcat cggccagggt gagcagttcg
     4801 tcgtacagtt cctgctgggt gaaaatcccc tcattcgcca gccgttccgg gctgagatag
     4861 agataacaga ggattttccc gaccatcata tccagttccg acaagacgga acgcgggata
     4921 agagtggtgg aacgtgggcg taaatagaag aacccttccg gtgcgcgaat aagctcaacg
     4981 ttataacgcg cgtaaaactc ttccagatat tcctgaaaat ccatcaggaa tgcatgatta
     5041 tccagttcgt cgaggccaat atggcggcct gaacgtaagg cgctgtccag cgccggaaat
     5101 aacggattcg ccagcgcctg cgccagctta accggcatca cttgttcaat atttgtcgat
     5161 gacatgcgcc tgtaccttgg ctccgtaatc attaatcggc tgccatttcg ctggcagtcc
     5221 ggtgaaatct gcttgcgcta cgccaagacg taccgcctga tcaataacaa tacgcgcaac
     5281 gtcaaagtga cgtgcacgcg gatactgtga cagatattcg cgtaccacca gaccaagatc
     5341 cagcggcact tgtctggttt tgtacacggc aagttgttct tcgatgatcg ccgccagctg
     5401 ttcgcggatc tcgttaaact cttcgtattc cagatcctca ggaagttccc cagtcacttc
     5461 ttcatcgcgc agtgccatct cttcgtcacg catatccagc agacgatcgg cattggcata
     5521 agttagcgcc cacggctcat caaaataggt ttgtaccgac tgacgtaacc gctgagcaaa
     5581 gacgcggttt ttatccatat cgatcgcggt acgaataaat ttgtgtacgt ggcggtcgta
     5641 gccaatccac aagtcgatgg attgctggcc ccaactgata atacgatcga gtttgctctg
     5701 cagatcgaac accagacgat cgacgaaatg cagatcgtca tgggtcatcg tcgcatcctg
     5761 aatgcgcaac agattagcct gcaatttgtc gcctgccgct tccagcgtat cctgcaattc
     5821 acgcagcgtt ccggaagttt ccgaaagcaa caattcacag ctggaaatcg ccgcccgcca
     5881 gtctttgttc agcaactggg cgatatcgtc cttcacctgc tgctgctgtt cgtccatcag
     5941 acgttgcgtc aggtcgatac tgtcgaaaat ttctgctacc gaatatttca gtggcgcata
     6001 gacattacgg tgccagtgaa attcatcacc gccctcttcg gcggcatctg ctgcgcgttt
     6061 gagctcaccc gccacaatcg acaactgcat agaaagacgc agcgtagaaa actcgcgctg
     6121 acggatgtag tagtcagtaa tgccgatgcc gagcggcgtc agacggtaaa ttgcgttccc
     6181 ttccgcctgc tcgctggtaa agcggttcag cagacgttga cgcaccatgt cgttgatcgc
     6241 gttattggcg cgcacgccga tggtttcgct ggtttgctca aacgcatcac tcacatggcg
     6301 gaatgcatcc accagctcgc cttcactcat ctcaccatcc agacgctcgc cgttcagcgt
     6361 ggcaaccgcc agcagaaaag agagtcggtc tacctgcagc gagatggaga agtcattttt
     6421 tctggcccag gcaaccagtt cggggactgt ctgggaaaat tcactcatac tttatccttg
     6481 ctctgcggtt tgcgcgcggt gacatgaata taacgcccca gggtaatata cggttcctga
     6541 cggcaataac gcgtttctaa ttcaagtaat gcttcatagc agtcgcgctg ctggtgcttc
     6601 tcgcgcagat aatcatgaaa cacgcgaacg cctgtcttac ccataatttg ccaaccagct
     6661 tcttccagcc acagataaac ctgcgccggg tcgcgtggat aatctggcga aagcgtccgt
     6721 tttttctttt tcggcattcc cgcctgcacg taatcaaaat tcccggcgac catgttatgc
     6781 atcaacaaac catgcgcatt gtagaacatt aacgacaaca cgccgcctgg acgcaacact
     6841 gaccacaggg tctgcaatac actgcgggga tcggccaccc actcgagcac cgcatggaac
     6901 aatatcagat caacgggcgt ttccaaatgc gaagcaacat cctgagcggc gcaatgtata
     6961 aattgcatgt tgtcgctcac accttttgct tctgccgcct gttttgcgcg gtcgatcatc
     7021 tgcgcagaaa gatcgcataa aatgacctga tgcccacgct cggccatttt gattgcggtc
     7081 tgcccttctc caccgccagc atccagcaca cgcagttttt gcgggcccat ttccgccagc
     7141 acgcgatcga gatcctgcca cagaatagcc tgtcgaagct gccctttggt ggtgccgtaa
     7201 atgttacggg aaaacttttc cgcaatatca tcaaaattgc gatcctgcat tgctttctcc
     7261 accggttggc taagacgttt tctgataaag acgctatttt gtcacagcca cggcgagaat
     7321 gaacccgtct ggtgtaatat cgctggcgat cccttgctat atggttaaaa aaggaaccag
     7381 aaaggatgct ttttacactg aaaaaagtga ttggcaatat gttgctgcca cttccgctga
     7441 tgttgttaat tattggcgct ggcctggcgc tgctgtggtt tagccgtttt caaaaaaccg
     7501 cgaagatttt tattagcatt gggtggctgg cactcttgtt attgagtttg caaccggtag
     7561 ccgaccgcct gctgcgtccc atcgaaagca cctatccgac atggaataat tcgcaaaaag
     7621 tggactatat cgtggtgctc ggaggtggtt atacctggaa cccgcagtgg gcaccgagct
     7681 ctaatttaat caataacagt ctgccacgcc tgaatgaagg tattcgctta tggcgcgaaa
     7741 atccgggatc aaaactgatc ttcacgggag gcgtagcaaa aaccaatacg gtgagtacag
     7801 cggaagtagg tgccagagtt gcgcaatcgc tgggcgtgcc gcgcgagcaa attatcaccc
     7861 tggatttgcc aaaagatacc gaagaagaag ctgcggcagt gaaacaggcg attggtgacg
     7921 cccctttcct gctggtgacc tccgcatcac atctgccgcg ggcgatgatc ttttttcagc
     7981 aggaagggtt aaatcctcta ccagccccgg cgaatcagtt ggccatcgac tcgccgctta
     8041 atccgtggga acgggcaatc ccttctccgg tctggctgat gcatagcgat cgtgttggct
     8101 atgaaacgct tggccgcatc tggcagtggc tgaaaggtcc gtcaggcgag ccacggcaag
     8161 agtgattttg atgccagatc gaagttgcgc cgactaaatc gtccggtatt aaccagttgc
     8221 gcaacttcat cccataacac atacaaccaa cgccgccaga tgaacgactc cgccactggc
     8281 gcgcgttgca ggtaactcca gagcaaatct tccgccagag aattatccat tagtcggaac
     8341 agttcgtatt ctcgcggtgc ccaaagcatt agtcccggcc cgaccatcgc cagtaactga
     8401 tcgctgcgcg aatctttcag catgctgcgt aaacagaagt taccgtgaat caacacacaa
     8461 ttgtcgttaa agccttcaaa taatgccggg agacattcgc gagtgcgaaa caggatccgc
     8521 ttatcctgca tcgtcagacc ggtgttattg aactgattga gcgtggtcca tagcacttca
     8581 acatgttgcc ggtaccatga gggccagaaa tgttcctgag tattgtcgac cgcgccgacg
     8641 caaccgcgac tgtcctgacg gtgccaggcc agtaaggctt caacgatttg gtctttgagc
     8701 tgttcccagc gttctggtgt tcgggctggt gcctccaccg aaacaccacg catccgctcc
     8761 agtagcagga catcagggcc ggggtgttct tcatgcgtca tcacaccata aatagtcggc
     8821 atacggacag tcccacttct tgccagcatg gtggttttcc atgccagttg tcgggctttt
     8881 ccgggcgtac taaagcttct tgccattaac ggcattgggt ttccctggct gtcatacaaa
     8941 gcccacaacg ccgtatccgc tttttcattg acgcactcaa tacgactgag tttttcgccc
     9001 agtaaatggc ttaattcggc acgcagctgt tccattcaga ttaccctcat aaaagctact
     9061 gctttcataa tgagcgccaa tataaaagat gtcacctgaa tgagatcaat tgttgaaaag
     9121 aaaaattgag gaaaatcgta acggtacgac actcctccca aaattggctg aagtgtcgtg
     9181 aagtgaaatt agcgcatttc agcgcgaacg cgctcaagat cttcaggggt atccacacct
     9241 gtgccaggaa cttcctgagc aacagcaaca tggatttttt caccgtacca cagaacacga
     9301 agctgctcta acatttcgat gtgttctaac ggacttgcct gccagttgac gtaacgacgg
     9361 ataaagcctg cacggtagcc ataaatgcca agatgacgca ggaagttatc gccaacggtt
     9421 tcaaggcctt ctgcaaaacg atcacgatcc caaggaatgg tggcgcgtga gaagtacagt
     9481 gcataccctt cagcgtcgag aaccactttc accgcattcg ggttaaacgc ttcttccgca
     9541 ttgtggattg gcaccgccag agtcgccata cccacctgac gctgagcgag gttatcagca
     9601 acctgacgaa tgattgtcgc agggatcatc ggttcatcac cctgcacatt aacgatcacc
     9661 gtgtcgtcgc tgaatgcgca tttttcgaca acttccgcca gacgttctgt tcctgactga
     9721 tgatcggcgc gcgtcataca tacttcaccg ccagcggctt caacggcgcg ggcaacatcc
     9781 tcatgatcgg ttgccacgat gatgcgctcg gcacctgatt cacgcgcgcg ttcaagaaca
     9841 tgaacaatca tgggtttgcc gttaatatca accaatggtt taccgggcag acgcgtggac
     9901 gcgtagcgcg cgggaataat gaccacaaaa ctcatgattt actctcatca gcagtcagca
     9961 cgcgggcttc cgtttccagc aacactggaa tgccatcgcg gagcgggaaa gccaggttat
    10021 ccagtttgca aataagctct tgtttttcct ggttatacca aagttttccg ttgcaaaccg
    10081 ggcaggcaat gatttcaagc aaacgatgat ccatagttcc tccagatgga tcgggttatg
    10141 aatgcataaa tcttatcata atcatttatg cgacggggtc tatttcccag ccagtgcggc
    10201 aatcagtaaa gagaccttgc gggcagcgtc ctaatgtcac ccgcgttgcc tgctgccagc
    10261 tcgcgaaatc agtaatcgcc tgacgtaacc ctttttgcag cattgtcgtt ggtttaatgc
    10321 cttcctgtaa ccacagagag ataacttcaa ggatgcctgt ctggcgatgc attttggcat
    10381 ccattcgccc aactaattgc ccacgatgta ataatggcag aacaaaatag ccatactggc
    10441 gtttcggcgc tggggtatag cactccagcc ggtagctaaa atcaaaaagc tgctctgcgc
    10501 gtttgcgatc ccagacaaca ggatcaaaag gcgaaagtac cgcgctgtgc gttgcagtga
    10561 gctttccggc cagcgcacgc tcgagtagcg gcagcaaatc agcatgcagc caaagattgc
    10621 ccaatttttc aacatgcaca gcaatgattt gctgctgttc agccctcgct tcgcgccacg
    10681 ccgccagtgc cggacgtttc agccgatagt aatctgccag ccactgttcg cggaatattc
    10741 ccaggctacg cgcactgtta tccagcatga tgatttctgc ttctgtttgc gaaacgagat
    10801 cgcgctcatc atcccagtca ggcatgacac ggtgggttaa atcataaacg cgctggaagt
    10861 tgcgccgttc aatcaccatc acctttccgg cagtaaataa accttccaga tgccgtttat
    10921 gcggtttcca ttcccaccag ccgcttgcac ctttacgagg atgctcaaaa tcggctgaac
    10981 gtaccggccc cttatcatga atatgctgaa ttaactgtgc aatttccgcc tcatgttcct
    11041 gcatccaggc gtctttgtat ttccagccca ttttttcaag tgccagcatg cggtggcgaa
    11101 taagacgaaa gtcgctacgc ggcataaagc aggcttcatg cgcccagtat tccattaatt
    11161 cgccacgcgc cagagactca tccagccact gggcaggata atttcccaga cgactgaaaa
    11221 gcaccagata tggactacgg gcaacaatat tgatggtatc gatttgcagc aaggacatgc
    11281 gggagatcgt tgccggaata tcctccaacg acgctcgacg gcggggtttg tttaacaggc
    11341 cttgtgcggc aaggtgaaga ttacgcgcat cagcaaggga gaggtgcggc agcgacattc
    11401 atgactccat caatcgaacg ctgccgcggc gtaactagtt gccagaagcc agcgaggtta
    11461 gttgcgtaag cagtttcgct ggttcatcac ctgaaagctg tgcgtctaca ggcaaatacc
    11521 accaattttc ttctgcaaag gcccggcatt tcaccgcatc tttttcagtc attaccagcg
    11581 tttgcccggc gcttaccaac gcactgacat ccgcatggtt caaagactga tgatcggcca
    11641 gcggtacaca tttttccggt tgtacgccac acatcttcag cgtggcaaaa aagcgcggcg
    11701 gatgcccaat ccccgccatc gccactacat gttcaagctg agcaacgtca caacgcgtac
    11761 cggtacgtaa attcaccgcc tgacccggca gcagatgcat gggaatttca ccgctgcgag
    11821 ggacaccacc gttgacgatt accgcatcaa ccgactttaa gcgccccgct cgctcacgca
    11881 ttggccccgc cggcaaccac cagccattgc caaagcgacg cacaccatca ataacgacaa
    11941 tttccacatc acgcgccaga cggtaatgct gtaaaccgtc gtcggttacg atgatctgca
    12001 catcagggtg ttgcgccaga atggctttta ccgcatcaga acgaacggga gaaaccgcaa
    12061 caggcgcatc agtgcgttga taaatcaaca caggttcatc acccgcctgt gctgttgtgg
    12121 tatctgccga caataacagc ggataagatt cagccttact accatatccc cgcgatacga
    12181 cccccacgcg aataccgcgc tgttgcaact gttccaccag ccagacaacg accggggttt
    12241 ttccgttgcc gcctgcggtg agattaccaa ccacgacaac cggtacgggg gcacgccagg
    12301 cgcgcttcag ttttagttta tagcaaagac ggatcgcgcc actcaccagg ccatacaacc
    12361 aggagagtgg cagcaatagc cgccacaaag gggattcacc agaccagatt ttttcgatca
    12421 ttggccaaac tgcattttgt gaagttgcgc gtaaacgccg cggtgctcaa gcaaatcgtt
    12481 atgcgtaccg cgttccacaa tgacaccatc ctcgacgacc acgatttcgt ctgccttttc
    12541 aatggtagac aagcggtggg caatcaccag agaggtacgg tttttctgca actcatccag
    12601 tgccgcctga atcgcacgtt cggattcggt atccagagcc gaggtagctt catccagaat
    12661 cagaatcggg ctatcacgca gcaaggctcg agcgatagca atacgctgac gctgaccgcc
    12721 agagagcagc acgccgtttt caccaatcac tgtatcgaga ccgttatcca tcttattgat
    12781 gaagtccatg gcgtaggcca tacgcgccgc ttcttcaatt tgctcacggc tgtactgttc
    12841 agtccgtgcg taagcaatgt tgttagcaac cgtatcgtta aacagatgga cattctgcga
    12901 caccagagca acctggttac gtaacgacgc cagggtatac tcgcgcagat cgtgaccatc
    12961 catcaggatt tcgccttcat caatatcgta aaaacgagtg atcaggctgg cgatggttga
    13021 tttacctgaa ccagagcgtc caaccagagc aaccgtcttc cctgccggaa ttttcaggtt
    13081 gatgttacgc aatgcaggta cgtcacgtcc cggataagta aaggtgacat tgcggaattc
    13141 cacgtcgcca gtcgcacgct cgatcacgcg cttaccttcg tctttctcct gctcactgtc
    13201 cagaatggta aacagcgtct gacaagccgc cataccgcgc tggaactggg cgttaacgtt
    13261 ggtcagtgat ttcagcggac gcatcagtgc aatcattgaa gagaaaacaa cggtaatcgt
    13321 accagcagtc aggctatcca tgacacttgg gaagctcgcc gcatacagaa caaacgccag
    13381 cgccaaagag gcgatcagct gaatgatcgg atcagagatg gaagaggctg aaaccatttt
    13441 catcccctga agacgcattc ggttgctgac tttatcaaag cgtttcgttt ccacttcctg
    13501 accaccgaaa atcaatactt ctttgtggcc cttcagcatt tgttcggcgc tggtggtcac
    13561 ctgccccatg gtgttctgca tgtttttact gatgttgcga aaacgcttcg atactacgcg
    13621 aatcgcaatc gaaacaatcg gtgccagcac aatcaaaatg atcgacagtt gccaactgta
    13681 atagaacatc atgatgaaca ggccgatgat tgacgcacct tcacgcacaa ccgttatcag
    13741 tgcgccggaa gaagaagaag caacctgttc ggaatcgtag gtaatacgtg acaacagcgt
    13801 ccccgttgac tgtttgtcaa aaaatgaaac cggcattccc atcatgtgac caaacaggcg
    13861 gcgacgcatg gtcattacca cctttcctga tacccaggag atacagtagc tggagacata
    13921 gctggtgata ccacgtaaaa tcatcagccc gatcaccacc agcggcatcc acaccagcac
    13981 ggagcgatct gttttaccaa agccatcatc aagaagtggc ttaaggagcg ataacatgaa
    14041 ggtatcgctg gctgcgttga ggattaacgc tacgcccgcc acgatcagac ccgctttaaa
    14101 aggcgcaatg gttggccaca gtcggcggaa cgtctgccac gtagagagat ctttgtcgtt
    14161 atgcattcaa aaaaccagca tttgttgaaa tagccgcata ttctacccgt tatccactgg
    14221 cacgccaaac cactgatgat accaacgagg taaaatttgc tctctgaggc tgctaatccg
    14281 ccagccttgc gctgaaaaat tgaccgtcac ttgaccctga tgtggagtat caagccattg
    14341 atatccttgc tgttgatagc gatgcttaac tttattagag ggcaatcgcc atgcgttata
    14401 gcgttatagc gcgatgccga tgcgagtgcc acttttccat tcactcgctg aattaatggc
    14461 aacgatgatg aggtattact gccatggtga ggtacctgaa gcaatgttgt ctgcacttgc
    14521 tgccagtaac ggcttagcat cttttgttca gcggggactt caatatcacc ggttagaaga
    14581 atgctattcg tcccgtcata aaccttaacc acacaggaat ggttatttcc tttatcgttg
    14641 ctagcttgta aaggccagtg cacgctgaaa cgcaatcctt gccattgcca cgcttcgcca
    14701 cgcacacagg gctgatgatg ttcccagttt aacggacttc tgatccataa catcggccat
    14761 gtgtgcaata ttgagtccag ccctccccgg tgatccagat gttcatgact cagaataacg
    14821 ccttccggtt caagattatg ccagtggagc caggggataa tcagttgttg cccactatcc
    14881 ccttcgggcc aggccagtcc tgtgtcatag agaatcgctt tgccgtttct ggctatcacc
    14941 attgccagcc cttgcccgac atcaagcatg tacacctgcc actcgtcagg tcgaggtttt
    15001 tgccacagcg gccagcacat cagcaagcct acagccacac acattgctgg caacgttcgc
    15061 caggcgttta atcgccatac caccagtaag aaccatgggg aaaatgatag ccattgccaa
    15121 cgttcagcaa tgttgatcca cccttctggc aaactcttta acccccagaa aagtaaagcc
    15181 aaagaccggt cggcaagaaa ccataaccct tgctccagga ttaacggccc gcttaaatgc
    15241 acaaccatcg cggcgaggat caacggaacc gtgataaatg tcaccaaggg aattgctaat
    15301 agatttgcaa taaacgaggt cagactaatg ccatgaaata tgacgatttg cacaggcata
    15361 agcaggagtg tgattcccag ttgcaaatga atgagggaaa caactgcacg caataccggc
    15421 ggcagttgcc actcaggaca gggaaaccac tggtaccaaa aaatcagtgc cgcgaccgca
    15481 gcggcagaga gccataaact ttgcgagaga atggcaacag gatccatcag caaaattgcc
    15541 gccagacaac atatccatac atcccagcca ctccactgtc gcccacttaa cttaagcatt
    15601 ccccacgtag caagcgccac cacggtacgc aatgcaggag gttgcattcc cgtcaaccag
    15661 gcataaaaag cagcacagca gattccacca attaatggca tttgccagtg gatccagcgc
    15721 ccaggcagaa aaacttgccc accgcgaatg agtccggcag ccagcaacgc cgcaaaagcg
    15781 atgtgcaatc ccgatatcgc cattaaatgc gccgttccag tatcacgcat gatatttttg
    15841 atttctttgg ggacggataa ccgttccccc atacctaaac caagaataac cgcattccac
    15901 atatagggtt gcagcgttgt ttgtagtgac gccagatact gtgcacgcag gctacaattc
    15961 ggttcaatga cacttgcctg cagaaagcgg ccgatgagcg gctgatgctg ggcaatggca
    16021 taacgctggc tatcaaagcc gccatcatta agttggccat gaactgcacg aactttgagt
    16081 ttcattgacc attgttgtcc agcacaaacc gcttgcggaa gatattcgcc atacagcaca
    16141 agacctggcg cagggaatat acgtcgacct tgtagatgag taatttgacc gtaatgagtg
    16201 gtcatgccgt cagttgcagt gatctcaaca attgcatcct gcgtcgcgcc agttaaggtt
    16261 tctcctgccc acaaaatttg ctttgctgat aatatgcccc aaacgaaaaa cagcaacgtc
    16321 agcgcgatat aacggacggt tttaacaggg ataaatgcca gtacgcaggc gaagagagtc
    16381 agaaacgcaa gggttaatgt cccaggcaat tggggcaaaa tcagcaacgg aaaaattccg
    16441 caaattatgc atacgccgac tgtcgttatt ttcatgcacc cctccctgtt tatggaaagt
    16501 gtgcaacttt gttgagcggt tgtctggtgg gaattttgcg ttccccgagg cacattcaga
    16561 agaatttaac gaaagggtga aaactgctca aaactctgcg aggcagcttc cagttcctga
    16621 ttagagaaat gaaaaaagca cccgacaggt gcttttctcg cgttcaagtt tgagtaaaaa
    16681 cttaaccgta aatattggcg cgatcgcgca gttctttacc aggtttaaag tgaggaacgt
    16741 attttccttc cagttctact ttatcgccag tcttcggatt acgtccggta cgtggtgcgc
    16801 ggtagtgcaa agagaaactg ccgaaaccgc ggatttcaat acgctcgccc tgcgcaagag
    16861 tcgaggccat atgctccagc atctctttta ctgcatcttc aaccgtcttg gcgggaatgt
    16921 gcgattgctg ggtggcaagt ctttctatca attctgactt ggtcatgatt cctccggttc
    16981 cttaaaggca aattaagcgg ctgctgttgc tgcattgatt aagggcggcc gtagccgccc
    17041 ttagtgcttg attacaggac gaaacctgca atctgtcaag taaactcaac aaacttcgga
    17101 ataaaaatcc cgaagagtca gagaattact cgcctttagc tgctttgaaa gcttcagcca
    17161 ttgcgttgtt ggagaagttt gcatcttcct gtttgttaac agttgcgatt gcatctttct
    17221 cgtcagcttc gtctttcgca cgaacagaca ggctgattgc gcggttttta cgatcaacgc
    17281 cggtgaattt agcttcaact tcgtcgccaa cgctcagaac cagggtagcg tcttcaacgc
    17341 ggtcacggga tgcttcagaa gcacgcaggt aaccttcaac gccgtcagcc agttctacgg
    17401 ttgcgccttt agcgtcaact gcagttactt taccggttac gatagcgcct ttcttgttca
    17461 gagcaaccca gttgttgaac ggatcttctg cgagctgttt aacgcccaag gagatacgtt
    17521 cacgttctgc gtcaacctgc agaacaactg cagcgatttc gtcgcctttt ttgtattcac
    17581 gaactgcttc ttcgcctgca acgttccagg agatgtcaga caggtgaacc aggccgtcga
    17641 tgccgccgtc taggccgatg aagataccga agtcagtgat agacttgatt ttaccttcaa
    17701 cacggtcgcc cttgttgtgg gtttccgcga actgctgcca cgggttagct ttgcactgtt
    17761 tcagacccag ggagatacga cgacgttctt cgtcgatatc cagaaccata acttccacta
    17821 catcgccaac gttaacaact ttggacgggt ggatgttttt gttggtccaa tccatttcgg
    17881 aaacgtgtac caggccttca acgccttctt cgatttcaac gaagcagccg tagtcggtca
    17941 ggttggtcac gcgaccagtc agtttggtac cttccggata acgtttagcg atagctaccc
    18001 acggatcttc gcccaactgt ttcaggccca gggatacacg ggtacgttcg cggtcgaact
    18061 tcagcacttt aacagtgatt tcgtcgccca cgttgacgat ttcgctcgga tgcttaacgc
    18121 gtttccaggc catgtcagtg atgtgcagca ggccgtcaac gccgcccaga tcaacgaatg
    18181 caccgtagtc agtgaggttc ttaacgatac ctttaacttc catgccttcc tgcaggtttt
    18241 ccagcagctg atcgcgctct gcgctgtttt cggattcgat aacggcacga cgagaaacaa
    18301 caacgttgtt gcgcttctga tccagcttga ttactttaaa ttcaagctct ttgccttcca
    18361 ggtgcagagt gtcacgcacc ggacgaacgt ctaccagaga acctggcagg aacgcacgaa
    18421 taccgttcag ctcaacagtg aagccgccct taactttgcc gttgataaca ccggtaacag
    18481 tttcagcatc ttcgtaagct ttttccagcg tgatccaggc ttcgtgacgt ttagctttct
    18541 cacgggacag cagagtttca ccgaagccgt cttctactgc gtccagagca acgtcaactt
    18601 cgtcacctac ctggatttcc agctcgccct gggcgttttt gaactgctca gccgggatgg
    18661 cggactcaga tttcagacca gcgtcaacca gtactacgtc tttgtcgata gcaacaacaa
    18721 cgccacgaac gatagaaccc gggcgggttt cgatttcttt taaggactct tcaaagagtt
    18781 gagcaaaaga ttcagtcatg tttaatcttc aggtttatat ttaacgtcca cctggctcca
    18841 tgccggatgg ggttgtttaa catacccgct gtcattccat tgcaacgggg gtactgcaaa
    18901 ttcggtcgct tatgcgagag ccaatttctg gcgcgcgtat tgtagcgctt tttcaatcac
    18961 ttgctcaatg cttaaggtgg tggaatccaa cactaaagca tcggctgccg gaaccagtgg
    19021 cgctaccgct cggttacgat cgcggtcgtc gcgttctttg atctcggcca aaaggcgctc
    19081 aaagttaaca ctaaagccct tctcctgcaa ctgtagcatg cggcgatgcg cacgttcttc
    19141 cgaggaggcg tcaaggaaaa ttttcactgg tgcatcaggg aataccaccg ttcccatgtc
    19201 gcggccatcg gcaatcagac ctggtaattc gcgaaacgcg cgttggcgac gcaataatgc
    19261 ttcacgaacg cgtgggaatg tcgcgacttg tgaagccgca ttcgccactt cctgagtacg
    19321 aatttcgccg ctgacatctt ccccttcgag gatcacttcc agattgccat tggttgacac
    19381 aaaacgtaca tccagatggg atgccagcgg taccagcgca tcttctgacg caacatcaac
    19441 atggtgatgt aatgccgcca atgccagtac gcgataaatt gcacccgaat ccagcagatg
    19501 ccattgcaat gcttccgcca tagccttaca caaggtgcct ttccctgcac cgcttgggcc
    19561 atcaatggta ataaccgggg caattgccgt catctttatc tccttaaaac aggcgtaccg
    19621 ttaacataac cgcgcgcatt atacgcgccg acgccacaaa tcgttaacgt tgtgtgcaaa
    19681 aaccagtttt gtctgtattc agatggggat ttattgcgca attatagcgg gctggcagaa
    19741 gaccagcccg aaagatgaca atgatttact taccagaggc gatacgatca cggatgtgtt
    19801 gcgcacgctc tgttgatggt gggtgagagt caaacatgga ctgggtgcga ccgccatcca
    19861 gactagccag tttttcaaag ctgccaacca gcccctaggt gctgatgcca cgtttcttca
    19921 acagatcaaa ggagaaatca tctgcttcgg actctttatc acgggagtat ttagcattaa
    19981 tagcgccttc tgcgatatca cccaattgtg agcgggaaag ctgggaagcc acaccgctgg
    20041 tagctgaaat ggcatcgcgt gcggcaacga tcgcataaga aactttcatt tcagccagcg
    20101 agtgacccaa cgcgacgtgg cccagttcat ggcccagaac accttcaatt tcgttgtcat
    20161 tcatcatgtc catcaggcca ctgtagacac ggacacagcc gttcgccatc gcccatgcgt
    20221 tgacgtcgct ggtcatataa acctatagtt gaccggcgtg ccattaatat tgttacccaa
    20281 tgctttggcg atttttgcca gacgtttacc gtacttgctg gatttacttg cgacttgatt
    20341 gccgctgtcc atttcggcac agccctgatt ggcaatcgct tttgcatcgg catcgcttaa
    20401 tgttgctgct ttataggcat ttaaaccgga gctgatagcc atattggtat caataccgtg
    20461 ggtattttga caccctgtca gtaatgctgc agaggtcgca atcgccagca gtaatttagt
    20521 attcttcatg tgaatcatcc gttgattaat catatataaa cattatgtat caacaactta
    20581 cattgacaag cgacgctacc attaatcaaa atgactcaca aggtccgaaa aaaaacgccg
    20641 acaatgtgc
//
LOCUS       DAJTSL010000064        20603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTSL010000064 DAJTSL010000000
VERSION     DAJTSL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.064"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(56..481)
                     /gene="nudI"
                     /locus_tag="OO433_002090"
     CDS             complement(56..481)
                     /gene="nudI"
                     /locus_tag="OO433_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6050570.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEYGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYTWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            760..1302
                     /locus_tag="OO433_002091"
     CDS             760..1302
                     /locus_tag="OO433_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050571.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARSTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1402..2604
                     /locus_tag="OO433_002092"
                     /pseudo
     CDS             1402..2604
                     /locus_tag="OO433_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
     gene            2824..3606
                     /locus_tag="OO433_002093"
     CDS             2824..3606
                     /locus_tag="OO433_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR6050572.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLSWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKSIACARDISRLLGWKSPFDLQAS"
     gene            3621..4826
                     /gene="rhmD"
                     /locus_tag="OO433_002094"
     CDS             3621..4826
                     /gene="rhmD"
                     /locus_tag="OO433_002094"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HCR6050573.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4883..6172
                     /locus_tag="OO433_002095"
     CDS             4883..6172
                     /locus_tag="OO433_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6050574.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6190..6993
                     /gene="yfaU"
                     /locus_tag="OO433_002096"
     CDS             6190..6993
                     /gene="yfaU"
                     /locus_tag="OO433_002096"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR6050575.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7034..7936)
                     /locus_tag="OO433_002097"
     CDS             complement(7034..7936)
                     /locus_tag="OO433_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6050576.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8129..9319)
                     /gene="glpC"
                     /locus_tag="OO433_002098"
     CDS             complement(8129..9319)
                     /gene="glpC"
                     /locus_tag="OO433_002098"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6050577.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9316..10575)
                     /gene="glpB"
                     /locus_tag="OO433_002099"
     CDS             complement(9316..10575)
                     /gene="glpB"
                     /locus_tag="OO433_002099"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6050578.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLH
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLLCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPHFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10565..12193)
                     /gene="glpA"
                     /locus_tag="OO433_002100"
     CDS             complement(10565..12193)
                     /gene="glpA"
                     /locus_tag="OO433_002100"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6050579.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12466..13824
                     /gene="glpT"
                     /locus_tag="OO433_002101"
     CDS             12466..13824
                     /gene="glpT"
                     /locus_tag="OO433_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6050580.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13829..14905
                     /gene="glpQ"
                     /locus_tag="OO433_002102"
     CDS             13829..14905
                     /gene="glpQ"
                     /locus_tag="OO433_002102"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6050581.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14947..15189)
                     /locus_tag="OO433_002103"
                     /pseudo
     CDS             complement(14947..15189)
                     /locus_tag="OO433_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15368..16018
                     /gene="inaA"
                     /locus_tag="OO433_002104"
                     /pseudo
     CDS             15368..16018
                     /gene="inaA"
                     /locus_tag="OO433_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(16072..16326)
                     /gene="yfaE"
                     /locus_tag="OO433_002105"
     CDS             complement(16072..16326)
                     /gene="yfaE"
                     /locus_tag="OO433_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6050582.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(16326..17456)
                     /gene="nrdB"
                     /locus_tag="OO433_002106"
     CDS             complement(16326..17456)
                     /gene="nrdB"
                     /locus_tag="OO433_002106"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6050583.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(17603..19888)
                     /gene="nrdA"
                     /locus_tag="OO433_002107"
     CDS             complement(17603..19888)
                     /gene="nrdA"
                     /locus_tag="OO433_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6050584.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGECP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4844 a         4992 c         5525 g         5242 t
ORIGIN      
        1 aattcgttaa ccactaaatt ttcaatctga cagccagtga tggctgtcat cattgttaaa
       61 gaagaccttt caaacgtaac gtttttcggg tggcaacatt caaatcataa tgcaccagat
      121 cttcaggttt tacccacgtg tagtcctgaa actcttcgtt tattttcact tctcggttgg
      181 cagaaacgca gtcaaaaatc aggtaaatca tataaatctc ttccttgcga ccatctgcat
      241 acgtcttggt gcgaatatca tcgctgaagg tccacggcgt gatttctgtc aaaagcagct
      301 gttctcccag ttcttcgcga atttcgcggc gtagtgcctc ttcaattcgt tcgccatact
      361 ccacgccgcc acccgaaagc gcccattgac cggggaaaac gccgcgatcg tcggccattt
      421 tgcacagcag ataagcacca tcattttgaa ttaaagggca tacaatagtc cgttgtcgca
      481 caggggtatt ccttataaat aaaaagaaac catgagaata cggtaactca gaaatatgtg
      541 aaagtgacag taatctcttt tgcgatgata actgcactta ttattatttt tgtgtgtatc
      601 ggtaaagtgt gtttattttt tcggcgttta attatttaag aagtatttag ttacaataag
      661 tgactagatc ccttttttcg ctcattgaat cctctgtgcc aggcagtaca atcccgcagc
      721 tttgggaatt ttgattaatt gcttgtggga aaggggataa tgaaaaaaat tgcgctagca
      781 ggtctggccg gaatgctgtt ggtttctgca tcggtcaatg caatgagcat cagcggtcag
      841 gcgggtaaag aatacaccaa tattggtgtc ggttttggga ctgaatcgac gggcctggcg
      901 ttaagcggta actggacaca taacgacgac gacggtgacg tcgctggcgt ggggctgggg
      961 ttgaatctgc ctctagggcc gttaatggcg accgttggcg gaaaaggcgt ttacaccaac
     1021 ccgaattacg gcgatgaagg ttatgccgca gcggtaggag gtggtttgca gtggaaaatt
     1081 ggcaacagct tccgtttgtt tggcgagtat tactactctc cggattcact ctccagcggt
     1141 attcaaagtt atgaggaagc gaatgctggc gcgcgttcca ccattatgcg tccagtcagt
     1201 attgaggcgg gttatcgcta cctgaatttg tcgggtaaag atggtaaccg cgacaacgcc
     1261 gtggctgacg gcccgtatgt tggggttaac gccagtttct gatcccttcc ggcgcggcat
     1321 tctgtcgcgc cattcagttt cttactacgc cttatctccc tttgcgttat agtgattcga
     1381 taacaaaagc aggagataac catgttaaaa gtggaaatgt tatccaccgg ggatgaagtg
     1441 ttacacgggc aaatcgttga cactaacgct gcctggctgg ccgatttttt ctttcatcag
     1501 gggctgccat tatctcgcct caatacggtg ggggataacc ttgatgactt agtcaccatt
     1561 cttcgcgaac gtagtcagca cgccgatgtg ctgatcgtta acggcgggct gggaccgacc
     1621 agcgatgatt taagcgcact cgccgctgcg acagcaaaag gtgaaggcct ggtgctgcat
     1681 gaagcctggc tcaaagagat ggaacgctat ttccacgaac gtggacgagt aatggcaccg
     1741 agcaaccgta aacaagcgga gctgcctgcc agtgctgaat ttatcaataa cccggtaggc
     1801 accgcctgtg gttttgccgt gcagcttaat cgttgcctga tgttctttac tcccggcgta
     1861 ccgtcagaat ttaaggtgat ggtcgagcac gaaatcctgc cgcgcctgcg cgagcgtttt
     1921 tctttaccac agccgccggt ttgtctgcgt ttaactacct ttggtcgttc ggaaagcgat
     1981 ctggcacaaa gcctggatac tctacaactg ccgccgggcg taacaatggg ctatcgctct
     2041 tcaatgccga ttatcgaact gaaactcacc ggaccggcaa gcgagcaaca ggcgatggaa
     2101 aaactgtggc tggacgttaa acgtgttgcc ggacagagcg tgattttcga aggcaccgaa
     2161 ggactgcccg tgcagatcag tcgcgaattg caaaaccgcc agttcagcct gacgttgagc
     2221 gagtaattca ccggtggttt attggctttg caactttctc gcgcaggtgc tccattgctg
     2281 gcgtgtgaag tggttccttc acaggaagaa aacctggcgc aaactgcgca ctggattaca
     2341 gaacggcggg ccaaccattt tgccgggctg gcactggctg tttcgggttt cgagaacgaa
     2401 catctcaact ttgcgctagc cacaccagac ggcactttcg ctctgcgtgt gcgtttcagc
     2461 actacgcgct acagcctggc tatccgtcag gaagtgtgcg caatgatggc actgaatatg
     2521 ctgcgccgtt ggttaaacgg ccaggatatc gccagtgagc atggctggat tgaggttatt
     2581 gagtccatga ccttatctgt ctgattccgc cactctgtgc cggtttactg caccggcaca
     2641 ttccccctcc ggcaaaactt gtccaacctg ttgcactcaa taagaaaaaa tccgtgcact
     2701 gattcacaaa tttgtgcgta atttctccag tccgctggac gaggagcggg atttgatgcg
     2761 cttcacaatt ttactccccc tgagcaacga gactgattcc agtatctaag actggagtcc
     2821 agtatgttgg aatcaagcaa agttcccgcc ctgacacggg ctattgatat tctcaatttg
     2881 attgcccgca ttggcccctg tagtgctgcg acgatcattg acacattggg aatccctaaa
     2941 agcacggcct atttgctgct taatgaactc aggcgtcagc gttttcttag ccttgatcac
     3001 caggaaaatt tttgtttgtg gaccaggctg gtggagcttt ctggtcatgc gttaagcaag
     3061 atggatctcc gggaactggc tcgaccgcgt ttgacgcaac tgatggatac caccgggctg
     3121 ttatgtcatc tggggattat tgataacggc agcgcttatt acattttaaa agtggagtca
     3181 tcagccacta tcagcgtgcg ttcccatgaa ggaaaaagcc tttcgcttta tcgctccgga
     3241 ataggtaaat gcctgctttc ctggcaacct gcagcggtac agcaaagtat tatcgaaggg
     3301 ctggtctggg agcaggcgac acccacaacg attactcatc cacagcaact tcacgaagaa
     3361 ctggcgcgta ttcgccgtca gggctggagc tatgacaacg gcgaagatta ccccgatgtc
     3421 cgttgtgtcg ctgcgccggt gtttaacgcc aataacgagc ttaccgctgc gatctcggtg
     3481 gtcggtaccc gcttacaaat caatgaagaa taccgtgatt atctggcggg caagtccatt
     3541 gcctgcgcca gggatatttc acgtctgttg ggatggaaaa gtccctttga cttacaagcc
     3601 tcataacaat ggagaacatc atgaccctac caaaaattaa acaggttcgc gcctggttta
     3661 ctggcggtgc gacagcagaa aaaggcgctg gcggcggtga ttatcacgac cagggggcga
     3721 atcactggat agacgatcat attgccaccc cgatgagtaa ataccgcgat tacgagcagt
     3781 cacgccagtc atttggcatt aacgttcttg gcacgttggt tgttgaagtc gaagcagaaa
     3841 acggccagac cggattcgct gtttcgacag ccggtgaaat gggctgtttt atcgtcgaaa
     3901 aacaccttaa ccgtttcatt gagggtaaat gtgtcagtga tatcaaactg atccacgatc
     3961 aaatgctcaa tgccaccctg tattactccg gctctggtgg cttggtgatg aatacgattt
     4021 cctgtgtcga tctggctctg tgggatctgt tcggcaaagt ggtcgggctt ccggtttata
     4081 aacttttagg cggcgctgtt cgtgatgaga ttcagttcta cgccacaggt gcgcgtccgg
     4141 atctggcaaa agagatgggc tttatcggtg gcaaaatgcc gacgcactgg gggccacatg
     4201 atggcgatgc ggggatccgc aaagatgccg ctatggtcgc ggatatgcgt gaaaaatgcg
     4261 gtgaagattt ctggttaatg ctcgactgct ggatgagtca ggacgtgaac tatgcgacca
     4321 aactagccca cgcttgcgcg ccctataacc tgaaatggat cgaagagtgc ctgccgccac
     4381 agcagtatga aggttatcgc gaactgaaac gcaacgcgcc agtcggaatg atggtcacca
     4441 gcggtgagca ccacggcaca ctgcaatctt ttcgtacgct ttcagaaacc ggtatcgaca
     4501 ttatgcagcc ggatgttggc tggtgcggtg gtttaaccac gctggtggaa attgccgcaa
     4561 tcgccaaatc cagggggcaa ctggtggtgc cgcacggttc gtctgtttac tctcaccatg
     4621 cggtgatcac cttcaccaat acgccattca gcgaattcct gatgaccagc ccggattgtt
     4681 caacgatgcg tccgcagttt gacccgattc tgctcaatga gccggttccg gtgaatggtc
     4741 gtattcataa atcagtgctt gataaacccg gttttggcgt cgaactcaat cgtgactgca
     4801 atttgaaacg cccctacagc cactaatcac tccgggcgtt gctgtgacaa cggtaacgcc
     4861 cacatattct aaggagcttt ttatgagcac cgctttgctt gacgccgtgg tgaagaaaaa
     4921 ccgtgtgcgt ttaattccgt ttatgttggc gctgtatgtg ctggcgtttc tcgaccgttc
     4981 gaatatcggt tttgccaaac agacctacca gattgatacc gggttgagta atgaagctta
     5041 tgcgctggga gcaggcattt tctttgtggt atatgcgttt ctgggtgttc cggcgaatct
     5101 tttgatgcgc aaactggggg ccagaacctg gattggtacg acaacactgc tgtggggatt
     5161 tctttcggca gccatggcat gggccgatac tgaagcgaaa tttctgatag ttcgcactct
     5221 gcttggtgct gcggaggccg gatttttccc tggtatgatt tatctcacct cgcaatggtt
     5281 tccgcagcgt aatcgcgcca gcattatggg gctgttctat atgggcgcac cgctggcgtt
     5341 aacactggga tcaccgcttt ccggcgcgct gttggagatg catggattta tggggcatcc
     5401 cggctggttc tggatgtttg tgattgaagg attgttggca gtcggtgctg gggtattcac
     5461 attcttttgg cttgatgaca caccggagca ggcacgtttt ctgagtaaac aagaaaaaac
     5521 gttgcttatc aatcaactgg caagtgaaga acaacagaaa gtgacttctc ggctgagcga
     5581 tgcgctgcgt aatggccgag tctggcaact agcgattatc tacctgacca ttcaggtggc
     5641 ggtttacgga ttaattttct tcctgccgac ccaggttgcg gcattgctgg gaacaaaagt
     5701 gggctttaca gcgtcggtgg tcaccgccat tccgtgggtt gcggccttgt ttgggacctg
     5761 gcttattccg cgctattccg ataaaaccgg cgaacggcgt aatgtcgcag cgctgacatt
     5821 actggcggcg ggcattggta ttggtctgtc cgggctgctt tctccagtaa tggcgatcgt
     5881 agcgctgtgt gttgcagcta tcgggtttat tgccgtgcag ccggtgttct ggacgatgcc
     5941 gacacaactg ctttccggta cggcgctggc tgcggggatt ggttttgtaa acctgtttgg
     6001 tgcagtgggc gggtttattg ccccgatcct gcgcgtgaaa gcagaaacgt tatttgccag
     6061 cgatgcggca ggattactga cactggcagc ggtggcggtc atcggttcgc tgattatttt
     6121 cactctgcgt gtaaatcgta ctgttgcgca gaccgacgtg gcacatcatt aaataggtta
     6181 aggaacacga tgaacgcatt attaaccaat ccctttaaag aacgtttacg caagggcgaa
     6241 gtgcaaattg gtctgtggtt aagctcgact acttcttata tggcagaaat tgccgctact
     6301 tccggttatg actggttgct gattgacggg gagcacgcgc caaacaccat tcaggatctt
     6361 tatcaccagc tacaggcggt agcgccctat gccagccaac ccgtgatccg tccggtggaa
     6421 ggcagtaaat cgctgattaa acaagttctg gatattggcg cgcaaactct attgatcccg
     6481 atggtcgata ctgccgatca ggctcgtcag gtggcgtctg ccacgcgcta tcctccctac
     6541 ggtgagcgtg gtgttggggc cagtgtggca cgggctgcgc gctggggacg cattgagaat
     6601 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     6661 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacca
     6721 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     6781 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     6841 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     6901 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     6961 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     7021 gggcctgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     7081 cgagcacctg gtcacgatca atgccctgtt ccagcatgcg caaagcaatt ttaatggctt
     7141 gttcatgcat accttcctgc cagccaattt gatgcccttc ctgccgtagc cgttcagcaa
     7201 tagtcattaa tctctccttt tgtagtggtg aacgttcggc aatctcctca ataaaacggg
     7261 tgaaacggga ggtatcgccg cattgcagca gataattaaa cagtgtttgt agctggctgt
     7321 cattagtgaa gcctctaacc aaaagcgtgg tgatcctgtc gaccatgccg attaaatcgc
     7381 ggtcgcgaat atgcttttga atcagttcca gcagagctat acgccgatgt tgcatgatct
     7441 catcgtcagg tacgatggtg atatccacca acggaaaagc ttcggtgtac aactgccggg
     7501 caagttgcgg atcgtcaaac tcatccagcc agttaagtga gtacgggtag ggcgatgttt
     7561 cgccatgata aaacagcaat ggcaccacca gcggaactct gtcatagcct ttatccaggt
     7621 gacgctgcat ggcggcagtg gcatagcgca ttagccgaaa agccatattc ttttctgcag
     7681 agctttgatg ttcaatcacg cagtagatat agccgtcacc gtcgctggtt tccacggacc
     7741 acaaaacatc cgagtagtaa gcgcgtaaac ttttttcaat aaaactagtg ggttccaggc
     7801 gtaaggtttg caggttgcaa agcttgcgca gtggttctgg taaatgtatt tcgagaaaat
     7861 cccgtgcggt ttcgggtgtg aacataaagg ttttaaatac cgcatcatgc ggggaggagg
     7921 ttgttgattc ggtcatggtt gtccgtcacc tgatatttat gatgacgcga cattacggtt
     7981 atcctaggcc tttgacatct gccagtttca gcctttgcga gacgttttca gaggaatttt
     8041 cactgcgttg caacgcatac attgggcacg gaatcgtgta cgcaaaaatg cccggtaacc
     8101 acatgcttac cgggcatcag aaaggagttt aagccagcgc ctgggccagt agcgtaatcg
     8161 gatgttcgca gcgaagactt gtggacatct caatctgcca tttacaggtt tcgcagtcgg
     8221 tgaccaccag atctgcgccg ctttcttcta tctggcggaa cagtggtgcg ccgatggctt
     8281 gtgaggtggg gtagttctct tttttgaaac cgtaagtacc cgcaataccg cagcactggg
     8341 aatccagcac cgttaactca agccccggga ttttacgcaa cagctccagg gtgtagagcg
     8401 tccagcccat tttttccata tggcacggag tgtgataaac cactttcagc ggcagcggtt
     8461 tcagcggtaa cgttttgcct tcgtccagct tgcgccacag ccagcgggtt gccagttcga
     8521 tatgatcgcg caagcctttg ttgtcgacat tcagcacttc cgggtattcg tcgcgcaggg
     8581 caaatgtaca ggttgaggag gtggcaatca ccggaatgcc ttttactccc acagcttcgc
     8641 ggatcgactc tacattcgta attgcctgtt tgcgtgcttt atcggtaaag ccgttggcga
     8701 ttagcggtac gccgcagcat ttttctttgc tgagcagttg tacaccggta cccattgcgt
     8761 tgagcacttt aattaaatct ttacctaact gcggatggtt gtagttaacg aagcagccgt
     8821 gaaagaaagc gacctggtct ttatattgtg cttgctgagc cgctatgctg cgataccagc
     8881 gacggaacgt gccgaaggag tatttcggta gcgtgcgacg atgatcgatt tttaacgccg
     8941 catcaagcag ctgccgcacc ggtttcagcg aggtagcggt gttgacgatt ggtgcgaacg
     9001 gcgtggaaac gctacccatc aggtcggtat gactcaacac aaaattacgc agcgacgggc
     9061 gcgtggtgtc atattttgcc cgcgcgcgct ggataatatc gccaatcttc acatcggacg
     9121 ggcaggcgac ttcacaacgt ttgcagttga tgcaatattt cagcgcctcg tcatacagtg
     9181 cgccatcttt caaacgcaga cgctcgccat ccggcccggc ttgttttggc cctggataac
     9241 cgggattcac ccggctcacc gggcaggcgg tggtgcagac ggtgcactta atgcagtttt
     9301 cgaagctggt gtcgttcatt gttggcctcc tgcgcgttgg gcaatctgtt gagcggcatg
     9361 taaagcactg acggcacaaa caccgccgcc gcatccctgg gcgatgggat caaatccgcc
     9421 cagcaccgaa ccgatggcaa acaggttctc aatggtttgc cctgcctgcg acgggcgtag
     9481 cgtctcatca gtggttacac cgaactgctg ccacggttgc ggcgcaaaaa aatcgccctt
     9541 ataccattca ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg gctcgcgaat
     9601 gccgttacgt tccgctacca gtccgccact aaagaaactg ccgctggcga gaaccgcgaa
     9661 atgtggacgt agcggaatat cggcgtgatt gcgggtccag atttcattca ctacgccatt
     9721 tttacaggtc acttttttca cttcatcgcc cggcatccac acgccaccct ggcgcacaaa
     9781 ctggcgctgt aactggtttt gcagacgaat gcccagcacg gaaggcggca gcgttggcaa
     9841 aagcatcagt gaacaaagta gtttttcatt caaccaacgc cacagtttgt catcggccag
     9901 gccgaagcag gcaggcatca ggatcatttc gcaggtattg gcgacaggaa taagcgcatc
     9961 aagtaacagc ggccagtttt cttcattatc aaggaaacgg gcgatattca cagcgcgaaa
    10021 ttcggtggcg ttattgcgca ggacatccag ttccggcagc tctatttctg cggtttcaac
    10081 cgaaagatcc agttcgtgca acgaagccgc cgcaagatgc gcctgaaaat ccatcaggcc
    10141 gcttatcccg accacacata ttttcttcgc gggcaacggc cagacgggga cttctggcga
    10201 acttagccag gtagagcgca gagtgccgag cggcgtaatc cgctggtgag ccagttctac
    10261 gctgccctgc aattgcgcac cgctttcagc gattaatgcc tgcgcctggc aagcgagatc
    10321 gagcacgcgt tgtggcccga gaagagagta aggatgtgca ggtgcctgct gacgcaaaga
    10381 ttccagtcca ctgtgaatgt ctgccacggg ttgaccatct ggcagatggc tcagcaaatc
    10441 cagcgaaccg gatgagaaat gcaacgcgct ttgaccacgc gtgacaatgg cacagcgcag
    10501 gccgtgtttt tgcagttgca ggccacagag taatccggcg aggccgccgc ccataatgac
    10561 agtatcaaag cgcatctttc tgctccttct ccagaccaca cagtccctga taaacccagc
    10621 gggtaaattc gctttcgcgc agtgcgtctc cccaggcgat gggttgcacg cctttccagc
    10681 gttcgttaag gaaggtggaa agttgctcga tagattgcgc ggacgtcgtg acgttaaacc
    10741 gttgcagcag tccggcagcg cggcaggcgc agagttcgcc ctggcaggtg cccatcccca
    10801 cacgggtacg acggcgtaaa tccagcaggc tattaacgtt taaattttct accgcgtact
    10861 gcacttcacc cgcagttacc gcttcgcact cacataccag gctacggtgc agacggcctt
    10921 cgctcagcca ggcaggcgtg cgatcgccat gacgataaac cgcagaaccg cgcagcgggg
    10981 caggcaggga gatgacttta cgcaaggtaa cttcagcggg ttcttgtgaa ccaggcagtg
    11041 ccagatcggc agtcgtacag gggcgcgtgt tgcccagttt gcggcatacc gcgtcggtag
    11101 cccattcagc catcagccga taggtcatca gtttgccacc ggtgatggtg ataaatccgt
    11161 ccagaccatc gcgttcagca tggtcgagca gcacgatgcc acggctgacg ttacgtccgc
    11221 tcggatcgtc atcgctggca accagcgggc gcacgccaga ataggcccgc aaaatgcgcg
    11281 ttttcgccat cacgggggcc agtttttccc cttcacgcag cagaatatca acctcttctg
    11341 ccgtcactcg attatcgtca atctcgttgt aatcaatacg taaagaggtg gtaccaatca
    11401 gcgaaatggt atcgccaggc accagaatat cggcgtcgga aggtttacgg cagcggttga
    11461 tcacatgctg gttaatgcgg tgatccatga tcagcagcga tcctttcgcc gggaacatgc
    11521 gaatgcgcag atcggcatat tcggcaatgt gttgccccca gatcccagcg gcattaacca
    11581 cgacaggtgc atgaagggcc tgagtttcgc cggtgagatg gttacgtaca cgaacaccgc
    11641 acaccgtcgc gccttcacga atcagccccg tgacttcatg agcggtaagg ataacggcac
    11701 cgtgttcttt ggcatccagc atgtttgctg cggtcagacg aaatggatca acggtgccat
    11761 ccggaacttt caccgcgcca atcagtgccg ggttaacggc aggttcgata atgcgcgctt
    11821 gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc gcaggcgcga ataaaagtgg
    11881 cctggaatga gaggtcatct tccggcaggg tgataaacag gccgttggtt ggttcaacgc
    11941 agtgacgtgc aatgcgtttc aggatctggt tttcactaat gcattcgcgg gccgattccg
    12001 catcggttac cgcatagcgc gcaccgctgt gcagcaggcc gtggttacgc ccggtggcac
    12061 cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag cccgcgcagg gcacagtcgc
    12121 gggcaatccc ggctcccgtt gcgccgccgc caatgataat cacgtcactt gattgcgagt
    12181 cgcgagtttt cattgttatc cctctgaagt tcgtttttta ccatttagcc atagtaaaaa
    12241 catgaattgt ttgatttcgc gcatgttcgc tcataattcg aaagtgaaac gtgatttcat
    12301 gcgtcatttt gaacattttg taaatcttat ttaataatgt gtgcggcaat tcacatttaa
    12361 tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac ggcaggttct ctcactgaat
    12421 caggctgtta atcataaata agaccacggg ccacggaggc tatcaatgtt gagtattttt
    12481 aaaccagcgc cacacaaagc gcgcttacct gctgcggaga tcgatccgac ttatcgtcga
    12541 ttgcgctggc aaattttctt ggggatattc tttggctatg cggcttacta tttggttcgt
    12601 aagaactttg cgcttgctat gccttatctg gtcgagcagg gattctctcg cggtgattta
    12661 ggtttcgcgc tttcggggat ctcgatcgct tatggatttt cgaaattcat aatgggttcg
    12721 gtatcagatc gctcgaatcc gcgcgttttc ctccccgcag gtttgattct ggcggcggca
    12781 gtgatgttgt ttatgggctt tgtgccatgg gcgacgtcga gcattgcggt gatgtttgta
    12841 cttttgttcc tctgcggttg gttccagggg atggggtggc cgccgtgtgg tcgtactatg
    12901 gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt cagtgtggaa ctgtgcgcac
    12961 aacgttggtg gtggtattcc gccgctgctg ttcctgctgg ggatggcctg gttcaatgac
    13021 tggcatgcgg cgctctatat gcctgctttc tgcgccattc tggtggcatt attcgccttt
    13081 gcgatgatgc gcgatacccc gcaatcctgt ggcttgccgc cgatcgaaga gtacaaaaat
    13141 gattatccgg acgactataa cgaaaaagcg gaacaggagc tgacggcgaa gcaaatcttc
    13201 atgcagtacg tactgccgaa caaactgctg tggtatatcg ccatcgccaa cgtgttcgtt
    13261 tatctgctgc gttacggcat cctcgactgg tcaccgactt atctgaaaga ggttaagcat
    13321 ttcgcgctgg ataaatcctc ctgggcctac ttcctttatg aatatgcagg tattccgggc
    13381 actctgctgt gcggctggat gtcggataaa gtcttccgtg gcaaccgtgg ggcaaccggc
    13441 gttttcttta tgacactggt gaccatcgcg actatcgttt actggatgaa cccggcaggt
    13501 aacccaaccg tcgatatgat ttgtatgatt gttatcggct tcctgatcta cggtcctgtg
    13561 atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa aagcggcagg tacggcagcg
    13621 ggctttaccg ggctgtttgg ttacctgggc ggttcggtgg cggcgagcgc gattgttggc
    13681 tacaccgtgg acttcttcgg ctgggatggc ggctttatgg taatgattgg cggcagcatt
    13741 ctggcggtta tcttgttgat tgttgtgatg attggcgaaa aacgtcgcca tgaacaatta
    13801 ctacaaaaac gcaacggagg ctaatggcat gaaattgacg ctgaaaaacc ttagcatggc
    13861 gatcatgatg agcactatag tcatgggaag cagtgcaatg gcggcggaca gcaacgaaaa
    13921 aatagtcatc gcccatcgcg gtgccagtgg atatttgccg gagcatacgc tgccagcaaa
    13981 agcgatggcg tatgcgcagg gagcggatta tctggaacag gatttggtga tgaccaaaga
    14041 cgaccatctg gttgttctgc atgaccatta tctcgatcgt gttactgatg ttgccgatcg
    14101 tttcccggat cgggcgcgca aagacggtcg ttactacgcg atagatttca cgctggatga
    14161 aattaagtcg ttgaaattta ccgaaggttt cgatattgaa aacggtaaaa aagtgcagac
    14221 ttatccgggg cgtttcccaa tgggtaagtc cgacttccgg gtgcacacct ttgaagaaga
    14281 gattgaattt gttcaggggt taaatcactc taccgggaaa aatatcggta tttatccaga
    14341 aatcaaagcg ccgtggttcc atcatcagga agggaaggat attgcggcaa aaacgctgga
    14401 agtgctgaag aaatatggtt acaccggtaa agacgataaa gtttatttgc aatgttttga
    14461 tgctgatgag ctgaagcgta ttaagaatga gctggaaccc aaaatgggca tggagctcaa
    14521 tctggtacag ctgattgcct ataccgactg gaatgaaacg cagcagaaac agccggatgg
    14581 aagctgggtt aattacaact acgactggat gtttaagccg ggtgccatga aacaggtggc
    14641 ggaatatgca gatggtattg gtccggatta ccatatgttg attgaggaga catcgcagcc
    14701 gggtaatatc aaactcactg gcatggtgca agatgctcag cagaataaac tggtagtgca
    14761 tccttatacc gtgcggtcag ataaactgcc tgaatacact cctgatgtga atcagttata
    14821 tgatgctctg tataacaaag cgggtgtaaa tgggctgttt actgatttcc ctgataaggc
    14881 agtaaaattt cttaataaag agtaatgtta ctgcaggatg gcgttcatgc gccatcctgt
    14941 ctcatatcaa atttttccaa tcgccactaa ttgttccatg cacatttgta gtaaaaacag
    15001 tggactgcct tctaccggct tttctttagc cagcaacgaa atctgtcgat agaaagttgg
    15061 ttcgagagga acggaacaca attcacctgc aatgcttttc agcgttaact ctggttgtaa
    15121 ggcgatgcct aatccctgtc gaataaaatt catcgctgta tccgcctgat taaattcaca
    15181 ttttatctgc tgggcgcact attagtggta gtggctggtg gaatctatgc gctgcaaatg
    15241 aagggtaaaa gcggtgtcaa gctagtagtg gcaaaagaaa ttcattaata cgacacgttt
    15301 cattaagatt ttcctcaggt aaccacctat agtcattgtc gttaataaaa acgacaggcg
    15361 atagggtatg gcagtttcag caaagtacga cgaatttaat cactggtggg caacagaggg
    15421 cgactgggtt gaagagccta actatcgccg taatggtatg agcggggtgc aatgcgtcga
    15481 gcgcaacggc aaaaagctgt atgtaaagcg catgacgcat catctgtttc attctgtacg
    15541 ttatccgttc ggccgaccaa cgattgtccg tgaggttgcg gtaattaaat aactggaacg
    15601 ggccggtgtc atcgtgccga agatcgtttt tggtgaagcg gtgaaaattg agggtgaatg
    15661 gcgagcgttg ctggtgactg aagatatggc ggggttcatc agcattgctg actggtatgc
    15721 ccagcatgca gtatcgcctt attctgacga agtacggcaa gccatgttga aagcagtggc
    15781 gctggcgttt aagaaaatgc atagcattaa tcgtcagcat ggctgttgtt atgttcgcca
    15841 tatctatgtg aaaacagaag gtaaggcaga agctggtttt cttgacctgg agaaaagtcg
    15901 tcgccgcttg cgtcgcgata aagcgattaa tcatgacttc cgccagctgg aaaaatatct
    15961 ggagccaatc cccaaagcgg actgggagca ggtgaaagcg tattactacg ccatgtaatc
    16021 gttgtaggcc agataagaca cataagcgtt gtatctggca ctacaaccaa ttcacatctc
    16081 gatttcaata tcgccttttg cccggcaaca acagggcaaa atttcccccg gctgaataaa
    16141 ggctaacggt tcggcaatcc agtcaacttg acctgcaacc aggcgtgtgc gacaggagcc
    16201 gcagtaacct tcgcgacact ggtactcaac cgccacattg tgggattcca gcgctgccag
    16261 aagggaaggg tgttcatcct ggcacagcag ttgtgtgcca gtgatgcgca gggtaacgcg
    16321 ggccatcaga gctggaagtt actcaaatcg tcggtgtcca cttccgagtc aatctgcccg
    16381 accagataag aactgacttc cacttcctgc ggagcaacct gcacgttatc agacaccagc
    16441 caagtgttga tccacgggat cgggttggag cgcgtctgga acggcagatc caaaccgact
    16501 gcctgcatac ggatattggt gatgtattca acgtactggc agagaatgtc tttattcaga
    16561 ccaatcatcg aaccgtcgcg gaacagataa tccgcccagt ctttctcctg ttgagctgcc
    16621 tgaacaaaca ggtcatagca ctcctgctta cactcttcgg caatttccgc catctcagga
    16681 tcgtccgcgc cgctgcgcag cagattcagc atatgctggg tgccggtcag gtgcagggct
    16741 tcgtcgcggg caatcaggcg aataattttg gcgttgcctt ccatcaattc gcgttctgca
    16801 aaggcaaagg aacaagcaaa gctgacgtag aagcggatcg cttccagcgc gttaacgctc
    16861 atcaggcaaa gatacagttt tttcttcagc tcgcgcaggc taacggttac ggttttaccg
    16921 ttaacggtgt gggtgccttc gcccagaagg tgccagtagc tggtcatttc gatcagttca
    16981 tcgtaatagc tggagatccc ttccgcacgt ttctggatct gctcgttggt gacgatatcg
    17041 tcaaacacaa cagacggatc gttaacgata ttacgaatga tatgagtata ggaacgggaa
    17101 tgaatcgttt ctgagaacgc ccaggtttcg acccaggttt ccagttccgg aatagaaata
    17161 agcggcaata gcgccacgtt cggactacga ccctgaatgg aatccagcag cgtctgatat
    17221 ttcaggttgc tgataaaaat gtgtttttcg tgctccggca gcgcctggta atcgatacgg
    17281 tcgcgggaga cgtcaacttc ttccggacgc cagaagaaag agagctgctt ttcgatcagc
    17341 ttttcgaaga tgtcatattt ttgctgatcg tagcgggcca cgttgaccgg ctgaccaaag
    17401 aacatcggtt ctttgagctg atcatttttc gtctgtgaaa aggtggtata tgccatgagt
    17461 gtgtcctgtt gggagtttaa tgccggataa ggcattttac gccgcatcct gtgcctgatg
    17521 cgacgctggc gcgtcttatc aggcctacaa atccgagccg taggccggat aaggcgttca
    17581 cgctgcatcc ggcatctcaa tatcagatct tacatgcgcc gctttcgcag ccatcgtcct
    17641 ggattgacgg taccagatcg tcctgcgcgt cttcagcgcc gtcacgggtg ttctgataat
    17701 acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc tttcagcaac tgctgcatcg
    17761 gcacttttcc tgacgggaaa cgtgacggat cgtagttggt gttagcagag atcgactgat
    17821 cgataaattt ctgcatgata cctaccagtt gcaggtagcc atcgttaccc ggcatttccc
    17881 acagcagctc ataggcgtcg tgcaggtgct cgtagtccgg caccacctgg cgcaaaatac
    17941 cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg ttcaataccg ttagtggcgt
    18001 tagagatctg cgaagaggtc tcggacggca tcagggcaga aagcgtggag ttacgcaggc
    18061 cgtgcgtttt gattgattca cgcaatgctt cccagtcgta atgcagcggc tcattagcga
    18121 tggcatccag atctttctta taggtatcga tcggcaggat ccctttcgcg taagtggttt
    18181 cgttaaacca cgggcactcg ccttgctctt tagccagctc attagacgct ttcagcaggt
    18241 aatactgaat cgcttcgaag gttttatgcg tcaggttgtt ggcgctgccg tcggagtagc
    18301 gtttaccgtg cttcgccagg tagtaagcga agttgatcac accaataccc agcgtacgac
    18361 gacccatcgc tccacgtttg gcggccggga tcgggtaatc ctgataatcc agcagcgcgt
    18421 caagtgcacg aaccgccaga attgccagct cttccagttc atccaggtta ttaattgcgc
    18481 ccaggttgaa agcagacagc gtacacagcg cgatttcacc gttctcgtcg ttgacgtcgt
    18541 tcagcggttt ggtcggcagg gctatctcca ggcacaggtt agactgacgc actggcgcga
    18601 tggccggatc aaacgggcta tgggtattac agtggtcaac gttctgaata tagatacgac
    18661 cggtagacgc acgttcctgc atcatcagcg agaacagctc aacggctttc acacgctgct
    18721 tgcggatgct gtcgtctttc tcatatttgg tatacagacg ttcaaactct tcctgatcgg
    18781 cgaagaacgc gtcgtacagc cccggtacgt cggacgggct gaacagggtg atatcttcac
    18841 ctttcagcag acgggtatac atcagtttgt tgatttgtac cccgtagtcc atatgacgca
    18901 cgcggttgcc ttccacacca cggttgtttt tcaacaccag caggctttcc acttccagat
    18961 gccacatcgg gtagaacagc gttgccgcac cgccgcgcac accgccctga gagcaggatt
    19021 tcaccgctgt ctggaaatgt ttgtagaacg gaatgcagcc ggtatggaac gcttcaccac
    19081 cgcgaatcgg gctacccagc gcacgaatac gcccggcgtt gatgccgatc ccggcacgct
    19141 gggaaacgta tttaacaatc gcgctggagg tggcgttgat ggaatccagg ctgtcaccgc
    19201 actcgatcag tacgcaggag ctgaactgac gagtcggggt acgcacgccg gacatgattg
    19261 gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta aaaacgcttc acatattgca
    19321 ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcggc aaccagaata taaaggaact
    19381 gggcgctctc atagatttcg ccggtcacgc ggttctgtac cagatatttg ccttccagct
    19441 gcttaacggc agcataagag aaggtcatat cacggtcgtg atcgataaac gtgtccatct
    19501 gcttgaactc ttcttccgtg tagtcttcca gcagatgatt atcgtatttg cccatctcga
    19561 ccattttcac cacgtggtcg tacagcgcag gcggctcaaa ctggccgtag gcttttttac
    19621 gcaggtggaa gatcgccagg cgcgcggcga gatactgata atccggcgca tcacgggaga
    19681 tcaggtctgc ggcagccttg ataatggttt cgtggatgtc agaggtcttg ataccgtcat
    19741 aaaactgaat gtgggagcgc agctcgacct gggaaatcga aacgttatgc agtccttctg
    19801 ccgcccaatc cagaacgcga tggattttgt cgagattgat gcgctctgtg ctaccgtcgc
    19861 gctttgtcac cagcagattc tgattcatgt atgtcgtacc tgtttttgga attctttccg
    19921 caatagtttc atgcagacta cctgtagtac gggaagctgc ttagaacact atatataggg
    19981 ggtatgtttg ggattcactg caagatagtg tgaaaatggc cctcttgcaa gtgcataact
    20041 ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac cttaggtcag acaaggtgtc
    20101 cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa ctgttcggcg atttcttgaa
    20161 cggtagaata ggttggataa attgatatag atggtttatt aatctttttg ctaatgattg
    20221 caaaaattga tctaagcacg agtcatcaca taatctgatg tatcagttgt ggtcaatata
    20281 taagcgaatg taagcgattg ttgatgtcga aatggttgtt ttctattcgg ttattgttaa
    20341 atgcgaagcc attttgttaa tttaccttat attataaaaa aattattcat tactttaatt
    20401 tagtttaaat ataactggta tcccttaagg ttattaactt gaattatgac gcactgatat
    20461 tattcatcaa ataataacaa aatagccatt gcatcgggtt ggacaattta caaaagaaag
    20521 ataacccata ttgtataatg cgtttaaata tgccgtctta tataaaatga tggtcagatt
    20581 aatatgcgga ttatctttct acg
//
LOCUS       DAJTSL010000065        20052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTSL010000065 DAJTSL010000000
VERSION     DAJTSL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.065"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..445
                     /locus_tag="OO433_002108"
                     /pseudo
     CDS             1..445
                     /locus_tag="OO433_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            467..1429
                     /locus_tag="OO433_002109"
     CDS             467..1429
                     /locus_tag="OO433_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6050585.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDVHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            1426..2473
                     /locus_tag="OO433_002110"
                     /pseudo
     CDS             1426..2473
                     /locus_tag="OO433_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(2565..3914)
                     /gene="accC"
                     /locus_tag="OO433_002111"
     CDS             complement(2565..3914)
                     /gene="accC"
                     /locus_tag="OO433_002111"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6050586.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(3925..4395)
                     /gene="accB"
                     /locus_tag="OO433_002112"
                     /gene_synonym="fabE"
     CDS             complement(3925..4395)
                     /gene="accB"
                     /locus_tag="OO433_002112"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6050587.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5373..6347)
                     /locus_tag="OO433_002113"
     CDS             complement(5373..6347)
                     /locus_tag="OO433_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6050588.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVMVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAIIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6499..8439
                     /gene="csrD"
                     /locus_tag="OO433_002114"
     CDS             6499..8439
                     /gene="csrD"
                     /locus_tag="OO433_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6050589.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKYPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8744..9787
                     /gene="mreB"
                     /locus_tag="OO433_002115"
     CDS             8744..9787
                     /gene="mreB"
                     /locus_tag="OO433_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6050590.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            9853..10938
                     /gene="mreC"
                     /locus_tag="OO433_002116"
     CDS             9853..10938
                     /gene="mreC"
                     /locus_tag="OO433_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6050591.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPTTGIAQPTPQQPTTGNAATA
                     PAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            10938..11426
                     /gene="mreD"
                     /locus_tag="OO433_002117"
     CDS             10938..11426
                     /gene="mreD"
                     /locus_tag="OO433_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6050592.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11435..12028
                     /gene="yhdE"
                     /locus_tag="OO433_002118"
     CDS             11435..12028
                     /gene="yhdE"
                     /locus_tag="OO433_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6050593.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAHKKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVVLADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12018..13487
                     /gene="rng"
                     /locus_tag="OO433_002119"
     CDS             12018..13487
                     /gene="rng"
                     /locus_tag="OO433_002119"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6050594.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13555..17355
                     /gene="yhdP"
                     /locus_tag="OO433_002120"
     CDS             13555..17355
                     /gene="yhdP"
                     /locus_tag="OO433_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6050595.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFHLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKLLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLSSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17511..18956
                     /gene="tldD"
                     /locus_tag="OO433_002121"
     CDS             17511..18956
                     /gene="tldD"
                     /locus_tag="OO433_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6050596.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVGHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVRGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19084..20013)
                     /gene="aaeR"
                     /locus_tag="OO433_002122"
     CDS             complement(19084..20013)
                     /gene="aaeR"
                     /locus_tag="OO433_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6050597.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
BASE COUNT         4761 a         4906 c         5497 g         4888 t
ORIGIN      
        1 attaatcatc ttattgctga ttaaccactt cattcttact aaaacaacct ttggacgaaa
       61 aatttatatc actggtggta ataaagaagc ggcaatttat tcagggataa atgtcacccg
      121 cttgaaaatt aaagtcttta tgattacagc cgtgctggca ggtatcagcg ggatgatcct
      181 cgcctcacgc ctctattccg ggtaaccgaa ttccgcgttg agctacgaac tggatgctat
      241 tgctgcggct gtactgggag gcaccagcct gaacggcggc tatggaaccg tggtggggac
      301 ggtgatcggc gcattaacta tcggcgttat taataatggc atgaacttaa tgaatgttcc
      361 ctatttttac cagatggtgg ttaaaggatt agtcattctt gtagccgttt attttgacgt
      421 aagaaataaa agaaaacgca gctaatcatt tcaaggaatt aatcatatga gtaaggtctt
      481 cacaattgga gaaattctgg ttgaaattat ggcgagtaaa atcggccagc catttgatca
      541 accaggtatc tggaacggcc cttatcccag tggtgcgccc gctattttta tcgaccaggt
      601 gacacgtctt ggcgttccgt gcggcatcat tagctgtgtg gggaatgatg gctttggtga
      661 tattaatata catcgtctgg ctgctgacgg tgtggatatc agggggattt cagtcttacc
      721 tctggaagct accgggagtg cctttgttac ttatcataac tctggcgatc gcgattttat
      781 ttttaatatt aagaatgccg cttgcggtaa attatcggcg cagcatgttg atgaaaatat
      841 tctgaaggac tgtacccatt ttcatattat gggctcttcc ctgttttcgt ttcatatggt
      901 agacgcagta aaaaaagcgg taactatcgt taaagcaaat ggtggtgtta tttctttcga
      961 ccctaatatt cgtaaagaga tgctcgatat tccagaaatg cgtgacgccc tccactttgt
     1021 tcttgagtta acggatatct atatgcccag cgagggcgaa gtgttgctct tgtcgccaca
     1081 ttcgacgccg gagcgtgcaa tcgcaggttt tcttgaagaa ggtgttaaag aggtgattgt
     1141 gaagcgcggc aatcaaggtg ccagttacta ctccgcgaat gagcagtttc atgttgagag
     1201 ctatccggtg gaagaagttg acccgaccgg tgcgggagac tgctttggtg gcgcgtggat
     1261 tgcctgccgc cagctagggt ttgacgtcca tcgagcgctg caatatgcca atgcctgcgg
     1321 tgcgctggcg gtaaccagac gtggcccaat ggagggaacg tctcgccttg cggaaatcga
     1381 agcgttcatc cagcgccatg acatgtccat tcgggaagca gcacaatgaa aaacgaaatt
     1441 tggtcgtgga ttggcgatct ctcgcaggtt gccggaatta aacactatga gttacgtagt
     1501 ggcagagcaa agggtacaga agcgtttgat gtccgtactg gtgcgggact ggcatttacg
     1561 gtggtaaaag atcgcgcgct ggatattgca tgggccagct acaaggacac ggcgctttct
     1621 tttatcaccc ccaacggcat tgtcgccccc gctttttttg agtctcaggg caacggattt
     1681 ttacgtagct tttacgcggg gttactgact acctgcggac ttagctacat tggcacgcct
     1741 tgtgaggatg aaggagaaac gctggggtta cacgggcgtt tggctgcgac tccagcagaa
     1801 gaggtggggt atcgcacaga acggaccgac gacggcattg aatttgtcat caatggcaaa
     1861 gtacgggaaa cgcgactgtt cggcgaaaat ctgacgctgg aaaggaccat tcgctgccgt
     1921 tatggcgaaa atgtgctgcg cattgaagac aaggtgacta atcatggttt cacccgccag
     1981 ccgctgcaaa ttctttatca ctttaattac ggctggcctt tattatcgcc acaggccaga
     2041 aatcttgctg tcggctaaaa gcgtaacgcc acgaactccg catgcggcgg aagggttaac
     2101 cagtcatctg gagatttgta ccccacagcc cggttttgac gagcaggttt actatctcac
     2161 gttgaatagc gacagtcagg ggatgagtaa agttgctctg gtgaacgccg aactggggtg
     2221 gggaatttac gagaaattcg acaccatgca gctacccaac tttatccagt ggaaaaattt
     2281 aggcgcgggt gagtatgtta tgggactgga ggtcagtaat agttttcctg acggtcgtga
     2341 taaagaacga gcgcaggggc gtctgccctt tattgagcca ggggaaacaa agaaatactg
     2401 ctttgagctg ggaattgttg atggcgatgc tgaaatgagc gcgttgaaag cggaaattgc
     2461 aggctaccgt tagctaacgg caataaaaac gccgggcagc gttaacaggc ccggcgttga
     2521 ttacgttctt tgctaagcgc gatgaaaaca ccgcgcgtca caaattattt ttcctgaaga
     2581 ccgagttttt tctccagata gtggatgtta gtgccaccat gctggaagtt ctcgtcattc
     2641 atgatgcgga tctgcagatc aacgttggtt ttgataccgt cgatgatcag ctcctgcagc
     2701 gcattcttca tgcgagcaat cgccacgtca cggttttcac cgtagcaaat cagcttaccg
     2761 atcattgagt catagtacgg cggtacggtg tagcccgcgt agatatgaga ctcccaacgc
     2821 actccaaaac cgccaggtgc gtggaaacgg gtgattttgc ctgggcttgg caggaaggtg
     2881 ttcggatctt cggcgttgat acgacattcc accgcatggc cgcgaacgtg aacttcttct
     2941 tgcttgatcg acagcggttg accggcagcg atacgcagct gttctttgat caggtcaacg
     3001 ccggtgatca tttctgtaac cgggtgttct acctgaatac gggtgttcat ttcgatgaaa
     3061 tagaactcgc cgttttcgaa caggaactcg aaagtacctg caccgcgata gccgatatca
     3121 acacacgctt tagcgcaacg ttcgccgatg tagcgacgta gttccggggt aatgcccggt
     3181 gctggtgctt cttcgaccac tttctggtgg cggcgctgca tggagcagtc acgttccgcc
     3241 agatagatag cgttgccctg accgtcagcc agtacctgaa tctcgacgtg gcgaggattt
     3301 tccaggtatt tctccatgta aaccatatcg ttgctgaaag cagctttcgc ttccgcacgg
     3361 gtcatggaga tggattgtgc cagttcagcg tcgccgcgca ctacgcgcat accgcgacca
     3421 ccaccgccgc cggaggcttt gataatcacc ggataaccaa tgcgtttagc aatggcacgg
     3481 tttttatcca tatcgtcgcc cagcgggccg tcagaacccg gtacgcaagg gacgcccgct
     3541 tttttcatcg ccgcgattgc ggatactttg tcgcccatca ggcgaatggt ttctgctttc
     3601 gggccaatga agataaagcc ggagcgttca acctgctcgg caaagttggc gttctcggag
     3661 aggaagccgt aacccggatg gattgctact gcgccggtga tttcagcggc gctgatgatt
     3721 gccgggatgt tcagataact ttttactgac ggagcagggc caatacagac cgtttcatct
     3781 gccagtaata cgtgttttag atcgcgatcc gcgctggagt gcacagcgac agtcttgatg
     3841 cccagttctt tacaggcacg aagaatacgc aatgcaatct cgccgcggtt ggcaataaca
     3901 attttatcca gcatgttcgc ctcgttactc gatgacgacc agcggctcgt caaattctac
     3961 cggttgtcca ctttcgacca gaattgcttt cacggtaccg gatttgtccg cttcgatctg
     4021 gttcatcatt ttcatggctt caacgatgca cagggtatcg cccacgttga ctttctgacc
     4081 cacttcgatg aacgcttttg cgtccgggct tggggtgcgg tagaaagtac caaccatcgg
     4141 ggagcgtacg atgtgaccac tgatttccgc tgctgctggc gcttccatgg aaggaacggt
     4201 cgccggagcg gctgcgttag attgagctgg ctgctgcatc attggtgcag cgtaagcttg
     4261 ttgcatcaca gggaaacttg cggcaggagc tgcacggcta atgcgtactg actcttcgcc
     4321 ttcagaaatt tccagttcgg agatgcctga ttcttcaacc agctcgatca gttttttaat
     4381 cttacgaata tccatgagtg ggttccgtac tctttgttta gtgtgattgt gacaggcgtt
     4441 tcaccgccgt ctgtaaagcg tatgccccga cagctgtatg catagcgata aattccagca
     4501 ggccgggacg ccggtctatt ttgccccaga gtatcaatgt tagacttgac ggacattgtg
     4561 cagcgatctg ctgctatcct ccggcaaaaa acaaaatata ccttcgttgc gccaatgtca
     4621 ccttttccgc agcgcaaaaa ctgcgtcagg gaggacgagg tcgcacatta taacgatttc
     4681 gtagcaattg gcagctaaat actggtctta tcagggaaga taatcaacag ctaacatgta
     4741 aataaccttc aacaccgtgt aatttgcaac aagccgcaca attcacgaaa ttagcgccat
     4801 catcgacgga acttcttata acgtaaggtg aaaagctgta aagccagccc tgcgaagatg
     4861 aggggctgca atgagataat cttcacagac cacaaataat gtagggatgc caggatcgcg
     4921 acaagataga cgacattacg cagcggttgc tggggattac ccaattttca ctgcatcgtc
     4981 tggatagatg tgaatgctaa agcacgcaaa atcaaccgac tgacaattct cagcattaaa
     5041 taggttcgag tggttaattt gcagtcaaat aaaatcagat tattcacccc atggtaccag
     5101 aaatcagtaa caggttaagc gtagtgtcgc ccaggcaaag caccataaac ctaacaggcg
     5161 acggaggcgt atcaataatg gctgtttaga ggagtatcaa tgcgcatcaa tttgtggtca
     5221 gtcgaaatca tgactttcca gtttggatga tgttgtacaa cttcctgacc aaggtgttcg
     5281 acgaaagctg tccggctaac cgcttttccc gttaaccgat tgaataatga tgtcaaaatg
     5341 agttccctct cttattattc cttgctaaat ggttagttaa ccttcaccag cgtgcgaccc
     5401 tggatctggt tattaatgat ggcctcggca aagttcggtg cctctgacag agatatctct
     5461 tttgccgcct gggtatagaa tgattccggt aaatcggcga ccagtcgctg ccaggcttgt
     5521 gcgcggcgtt ctggtggcgt cattactgaa tccacccctt gcaaacggac attacgcaga
     5581 ataaatggca tgaccgtggt tggcagagta aaaccacccg ccagaccaca ggccgccacg
     5641 cagccgccgt aattcatttg cgccagcact tttgccagca ctttgtcgcc aacagtgtca
     5701 attgccccag cccagacttg tttttccaga ggacgggatt cggcaaactc atcacgaggg
     5761 agaatacggc tggcacctaa acttttcaga tattcatggg tactttcgcg accggaaacg
     5821 gcaacgacct gataacccaa cttatgcagc agcgccacgg cggtactgcc gacgccacca
     5881 ctggcacccg tcacgacaat ctccccgtcc tgcgggcgaa caccggcatc ttccagcgcc
     5941 atcacacaca gcatggcggt aaaaccggca gtaccgataa ttattgcttt acgcgcgtcc
     6001 agcccttgcg gcatggcaac cagccagtca cctttcactc gcgcctgctc cgccagccca
     6061 ccccagtggt tttcaccaac gccccagcca gtgagtaaca cctcctgacc ggcatgaaaa
     6121 cgcggatctt cgctggtgcg tacagttccg gcaaaatcga tcccaggaat catcggaaaa
     6181 ttacggatga tttttccctt accggtaatc gccagcgcat ctttatagtt caggctcgac
     6241 cagtgaacat cgaccatgac atcgccctcc ggcaggcgac tttcgtccag agtctgtact
     6301 gatgcgagag ttttgccgtc ctgctgttct aaaagtaacg cctgcataag tggtcctcat
     6361 gtgcatgatg gattggaaaa taatttctga agattatact cgctaatgga aaagcaaagc
     6421 cgatgaagcg caataaattg cgagataaat ctgatttgct agtatgcccg cttcctcact
     6481 atcggagtta acacaaggat gagattaacg acgaaatttt cggcctttgt tacgctgctc
     6541 accgggttaa caatttttgt gactttgctg ggctgttcgc taagtttcta caacgcaatt
     6601 cagtataagt ttagtcatcg cgttcaggcg gtggcgacgg cgattgatac ccaccttgtg
     6661 tcgaatgact tcagcacatt aaggccacaa attaccgaat taatgatgtc ggcagatatc
     6721 gttcgtgtag acctgctcca tggtgataaa caggtttata ccctggccag aaatggtagt
     6781 tatcgtccgg ttggctccag cgatctgttt cgtgaactga gcgttccgtt gataaagtat
     6841 ccggggatgt cgctgcgtct ggtttatcag gatccgatgg gcaactattt ccattcgttg
     6901 atgaccaccg cgccgctcac gggggcgatt agctttatca ttgttatgct cttcttggcg
     6961 gtacgctggt tacaacggca acttgccggg caagaattgc tggaaacccg ggctactcgt
     7021 atcttaaacg gtgagcgtgg ctctaatgtg ttgggaacca tctatgaatg gccgcccaga
     7081 accagcagtg cgctggatac gctgcttcgt gaaattcaga acgcacgcga acaacacagc
     7141 cgtcttgata cgctgatccg ctcttatgcc gcccaggacg tgaaaaccgg cctcaataac
     7201 cgactctttt tcgataatca gttagcaacg ttactggaag atcaggagaa agtaggtacc
     7261 cacgggatcg tgatgatgat tcgtctgccg gatttcaata tgttgagcga tacttggggg
     7321 cacagccagg ttgaagaaca gttcttcact ctgacgaatc tgctgtcgac atttatgatg
     7381 cgctaccctg gcgcactgct ggcgcgttac caccgcagtg attttgctgc gctgttaccg
     7441 caccggacgt taaaagaggc agagagcatc gccggtcagt taatcaaagc cgtcgatacc
     7501 ttgccgaaca ataaaatgct cgatcgcgac gatatgatcc acattggtat ctgcgcctgg
     7561 cgtagtggtc aggataccga gcaggtaatg gaacatgcag agtctgccac gcgtaatgcg
     7621 ggattgcagg gcggcaatag ctgggctatt tacgatgact cgttgcctga aaaaggacgc
     7681 ggtaatgttc gctggcgtac gcttatcgag caaatgctca gtcgcggcgg cccgcgcctt
     7741 tatcaaaaac cggcggttac tcgcgaaggt caggttcatc atcgcgaact catgtgccgc
     7801 atcttcgatg gtaatgaaga ggttagctcg gcggagtata tgccgatggt cttgcagttt
     7861 ggcttatcgg aagagtatga ccgtctgcaa atcagccgtc ttattccact attgcgttac
     7921 tggccagagg aaaatctggc gattcaggtt accgttgagt cgctgattcg cccgcgtttt
     7981 cagcgttggc tgcgcgatac gttaatgcaa tgtgaaaaat cacaacgaaa acgcataatt
     8041 attgaacttg cagaggccga tgtaggtcaa catatcagtc gtttacaacc tgttattcgt
     8101 ttagtgaatg ctttaggggt acgggtagcc gtcaaccagg ctggtttgac gctggtaagc
     8161 accagttgga tcaaagaact taatgttgag ttactcaagc tccatccggg gctggtcaga
     8221 aacattgaga agcgaacgga gaaccagctg ctggttcaaa gcctggtgga agcctgctcc
     8281 gggaccagca cccaggttta cgccaccggt gtgcgttcgc gaagcgagtg gcagaccctg
     8341 attcagcgcg gtgttacagg cgggcaaggg gattttttcg cgtcctcaca gccacttgat
     8401 actaacgtga aaaaatattc acaaagatac tcggtttaac ctgccgttta atccgttttc
     8461 acgtagaata acgcgcgctg cgtctcatgg gagtgtgctt gtctgctcgc cagattgttg
     8521 cagcacatat gcagatgaat gaccttacgc ggttgcaaac aggcgaggaa tgctgctgat
     8581 gcattaagcc tttctggact caggcagaga tttgtaacaa aggaaacgaa ctgcactaat
     8641 tttcaccgta gcagatgatt tttgcgcctt gtcgctgctg cgtgtggttg gtaaagtaag
     8701 cggattttct tttccgcccc agctttcagg attatccctt agtatgttga aaaaatttcg
     8761 tggcatgttt tccaatgact tgtccattga cctgggtact gcgaataccc tcatttatgt
     8821 aaaaggacaa ggcatcgtat tgaatgagcc ttccgtggtg gccattcgtc aggatcgtgc
     8881 cggttcaccg aaaagcgtag ctgcagtagg tcatgacgcg aagcagatgc tgggccgtac
     8941 gccgggcaat attgctgcca ttcgcccaat gaaagacggc gttatcgccg acttcttcgt
     9001 gactgaaaaa atgctccagc acttcatcaa acaagtgcac agcaacaact ttatgcgtcc
     9061 aagcccgcgc gttctggttt gtgtaccggt tggcgcgacc caggttgaac gccgcgcaat
     9121 tcgtgaatcc gcgcagggcg ctggtgcccg tgaagtcttc ctgattgaag aaccgatggc
     9181 tgccgcaatt ggtgctggcc tgccggtttc tgaagcgacc ggttctatgg tggttgatat
     9241 cggtggtggt accactgaag ttgctgttat ctccttgaac ggcgtggttt actcctcttc
     9301 cgtgcgcatt ggtggtgatc gtttcgacga agctatcatc aactatgtgc gtcgtaatta
     9361 cggttctctg atcggtgaag ccaccgcaga acgtatcaag cacgaaatcg gttcggctta
     9421 cccgggcgat gaagtccgtg aaatcgaagt tcgtggccgt aacctggcag aaggtgttcc
     9481 acgcggtttt accctgaact ccaacgaaat cctcgaagca ctgcaggaac cgctgaccgg
     9541 tattgtgagc gcggtaatgg ttgcactgga acagtgcccg ccggaactgg cttccgacat
     9601 ctccgagcgc ggcatggtgc tcaccggtgg tggcgcactg ctgcgtaacc ttgaccgttt
     9661 gttaatggaa gaaaccggca ttccagtcgt tgttgctgaa gacccgctga cctgtgtggc
     9721 gcgcggtggc ggcaaagcgc tggaaatgat cgacatgcac ggcggcgacc tgttcagcga
     9781 agagtaatcg gatgcaggca ggggaaatgt ctgtttaccc tgcctagtct gatacgagaa
     9841 tacgcataac ttatgaagcc aatttttagc cgtggcccgt cgctacagat tcgccttatt
     9901 ctggcggtgc tggtggcgct cggcattatt attgccgaca gccgcctggg gacgttcagt
     9961 caaatccgta cttatatgga taccgccgtc agtcctttct actttgtttc caatgctcct
    10021 cgtgaattgc tggatggcgt atcgcagacg ctggcctcgc gtgaccaact agaacttgaa
    10081 aaccgggcgt tacgtcagga actgttgctg aaaaacagtg aactgctgat gcttggacaa
    10141 tacaaacagg agaacgcgcg tctgcgcgag ctgctgggtt ccccgctgcg tcaggatgag
    10201 cagaaaatgg tgactcaggt tatctccacg gttaacgatc cttatagcga tcaagttgtt
    10261 atcgataaag gtagcgttaa tggcgtttat gaaggccagc cggtcatcag cgacaaaggt
    10321 gttgttggtc aggtggtggc cgtcgctaaa ctgaccagtc gcgtgctgct gatttgtgat
    10381 gcgacccacg cgctgccaat ccaggtgctg cgcaacgata tccgcgtaat tgcagccggt
    10441 aacggttgta cagatgattt gcagcttgag catctgccag cgaatacgga tattcgtgtt
    10501 ggtgatgtgc tggtgacctc cggtctgggc ggtcgtttcc cggaaggcta tccggtcgcg
    10561 gttgtctctt ccgtaaaact cgatacccag cgcgcttata ctgtgattca ggcgcgtccg
    10621 actgcaggac tgcaacgttt gcgttatctg ctgctgctgt ggggcgcgga tcgtaacggc
    10681 gctaacccga tgacgccgga agaggtgcat cgtgttgcta atgaacgtct gatgcagatg
    10741 atgccgcagg tattgccttc gccagacgcg atggggccaa agttacctga accgacaacg
    10801 gggatcgctc agccgactcc gcagcaaccg acgacaggaa atgcagctac tgcgcctgct
    10861 gctaatcgct ctccacaaag ggctacgccg ccgcaaagtg gtgctcaacc gcctgcgcgt
    10921 gcgccgggag ggcaatagtg gcgagctatc gtagccaggg acgttgggta atctggctct
    10981 ctttcctcat tgcgctgttg ctgcaaatca tgccctggcc ggataacctg attgttttcc
    11041 ggccaaactg ggtgttactt atcttgttgt attggatcct ggccttgccc catcgcgtaa
    11101 atgtgggcac aggttttgtg atgggtgcca tactggatct gatcagcggc tcgacgcttg
    11161 gcgtacgcgt attggcgatg agcatcattg cttacctggt ggcgctgaaa taccagcttt
    11221 tccgcaacct cgcattatgg cagcaggcgc tagtcgtcat gttgctttcg ctggtggtgg
    11281 atattattgt tttctgggca gagtttttag tgattaacgt ctctttcaga ccggaagtgt
    11341 tctggagtag tgtagtcaat ggggtgctct ggccgtggat tttcttgctg atgcgtaaag
    11401 tccgtcagca gtttgcagtg caataaaggt ttctatgact tctctgtatt tagcttccgg
    11461 ttctccgcgt cgtcaggagt tacttgcgca acttggcgtg acctttgaac gtattgttac
    11521 gggcattgag gagcagcgtc agccgcagga gagcgcgcag cagtatgttg tgcgtctggc
    11581 acacaagaaa gcacaggcgg gtgtcgcgca aacggcgcag gatctcccgg tgctgggtgc
    11641 ggatactatc gttatcctga atggagaagt gctggagaaa ccgcgcgacg cagagcatgc
    11701 ggcgcagatg ttgcgcaaat tatcgggtca gacccatcag gtgatgacgg cagtggtgtt
    11761 ggccgacagc cagcacattc tcgattgcct ggtggtcacc gatgtgactt tcagaacgtt
    11821 aacagacgaa gacatcgcgg gctatgtcgc cagcggtgaa ccgttagata aagcaggtgc
    11881 atacggtatt caggggctgg gtggctgttt tgtcaggaag ataaatggca gctatcacgc
    11941 cgtagtcggc ttaccgctgg ttgaaacgta tgaattatta agtaatttta acgcactgcg
    12001 tgagaaaagg gataaacatg acggctgaat tgttagtaaa cgtaacgcct tcggaaacgc
    12061 gagtggcgta tattgatggc ggtattctgc aggaaattca tattgaacgt gaggcgcgac
    12121 gcggaatagt aggcaatatc tacaagggtc gtgtaagtcg tgtacttccg ggtatgcagg
    12181 cggcttttgt agatattggg ctggataaag ccgcgtttct tcatgcatcc gatatcatgc
    12241 cgcacaccga atgtgtggcg ggtgaagaac aaaagcaatt cacggtgcgc gacatctcgg
    12301 aactggttcg tcaggggcaa gatctgatgg tgcaggtggt gaaagatccg cttggcacta
    12361 aaggtgcgcg cctgaccacc gatatcacgc tgccttctcg ctatctggtg tttatgccag
    12421 gggcttctca cgttggggtt tcccaacgta ttgaaagcga atcagaacgt gaacgcctga
    12481 aaaaagtggt cgcagagtat tgcgacgagc agggcgggtt tatcatccgt accgcagcgg
    12541 aaggggttgg cgaggctgaa ctggcctccg atgccgctta tctgaaacgc gtctggacca
    12601 aagttatgga gcgtaaaaaa cgcccgcaga cccgttatca gctgtacggc gaactggcgc
    12661 tggcgcagcg tgttctgcgt gatttcgccg acgccgaact ggaccgcatt cgcgttgact
    12721 cacgcctgac ttacgaagca ttgctggagt ttacctcgga gtacattccc gagatgacca
    12781 gcaagctgga gcattacacc ggacgccagc cgattttcga tctctttgat gtcgaaaacg
    12841 aaatccagcg agcgctggaa cgcaaagtag aactgaaatc cggtggttat ctgattatcg
    12901 accagaccga agcgatgacc accgtggaca tcaataccgg agcgtttgtc ggtcatcgca
    12961 atctggacga caccattttc aataccaata ttgaagcgac gcaggctatc gctcgccagt
    13021 tacggttgcg taatctgggt gggattatca ttattgattt catcgatatg aataatgaag
    13081 atcaccgccg ccgcgtgctg cactcgctgg agcaggcgtt gagcaaagac cgggtgaaaa
    13141 ccagcgttaa tggtttttcg gcgctggggc tggtggagat gacgcgtaaa cgcacccgcg
    13201 aaagcattga gcacgtactg tgtaacgaat gcccaacctg ccacggtcgc ggaacggtga
    13261 aaaccgtgga aacggtatgc tatgaaatca tgcgcgagat tgttcgtgtc caccatgctt
    13321 acgactccga ccgtttcctg gtctatgctt ctccggcagt agctgaagcc ttgaaaggcg
    13381 aagagtcaca ctcgctggcg gaagtggaaa ttttcgttgg caaacaggtt aaagtacaaa
    13441 ttgaaccgct ctataaccag gagcagtttg acgtcgtaat gatgtaaaca gatgctgggc
    13501 cgccatccgg caaagggttt ttgagtcaca tttttagcag acaaggagtg acgggtgagg
    13561 cgattgccgg ggattttact gcttactgga gccgcgctcg ttgtgatcgc agccctgctg
    13621 gttagcggcc tgcgtattgc tttaccgcat cttgacgcct ggcgtccgga aatcctcaac
    13681 aaaatagaat ccgcgactgg catgccggta gaagccagtc agctctcagc cagctggcag
    13741 aattttggcc cgacgcttga agcacacgac atccgtgcag aactaaaaga tggcggcgaa
    13801 ttttcggtta aacgcgttac tctggcgctg gatgtctggc agagcttgtt acatatgcgc
    13861 tggcagtttc gcgacctcac tttctggcag ctgcgctttc gcaccaacac tcctatcacc
    13921 agcggtggtg gtaatgatag cctggaagcc agtcacatca gcgatctgtt tcttcgtcaa
    13981 tttgaccatt tcgatctccg cgacagtgaa gtcagttttc tgacgccatc cggtcagcgc
    14041 gccgagctgg cgatcccaca actcacctgg ctgaacgatc cacgtcgaca ccgtgcggaa
    14101 ggcctggtaa gcctctccag ccttaccgga cagcacggcg tgatgcaggt gcgcatggat
    14161 ttgcgcgatg atgaggggtt gttaagcaat ggtcgcgtct ggctacaggc ggatgacatc
    14221 gacctgaagc cgtggctcgg taaatggatg caggacaata ttgctctgga aacggcacag
    14281 ttctcccttg aaggctggat gacgatcgac aaaggcgatg taaccggcgg tgacgtctgg
    14341 ctgaaacagg gcggtgccag ctggttgggc gagaagcaaa cgcatacgct gtcggtggat
    14401 aatctgaccg cgcatattac gcgtgaaaat ccgggctggc agttctctat tcccgataca
    14461 cggatcacga tggacggcaa accctggccg agcggagcat tgacgctggc ctggataccg
    14521 gaacaggacg ttggcggcaa agacaataaa cgcagtgacg aactccggat tcgcgccagt
    14581 aatctggagc tggcaggcct ggagggcata cgcccgctgg ccgcgaaact ttcacctgca
    14641 ctgggtgatg tttggcgctc cacacaaccg agcggcaaga ttaacactct ggcgctggat
    14701 atcccgcttc aggcggcaga caagacccgt tttcaggcat cgtggagcga tctggcctgg
    14761 aagcaatgga aattattacc gggtgcggaa cacttctccg ggacgctttc cggcagcgtt
    14821 gaaaatggtt tgcttaccgc gtcgatgaag caggcaaaga tgccttacga aacggtattc
    14881 cgtgcgccac tggaaatcgc cgacggccag gcaactataa gctggctgaa caatgacaaa
    14941 ggtttccacc tggatgggcg taatattgac gttaaagcca aagctgtcca tgcgcgcggc
    15001 ggttttcgtt acctgcaacc tgctaacgat gaaccctggc tgggtattct ggctggcatc
    15061 agtaccgatg atggttcaca agcctggcgc tatttcccgg aaaacttgat gggtaaagac
    15121 ctagttgatt acttaagtgg cgcgattcag ggcggtgaag cggataacgc gacgctggtt
    15181 tatggtggca atccgcaact cttcccctat aaacacaacg aaggtcagtt tgaagtgctg
    15241 gtgccgctgc gtaacgcgaa gtttgccttc cagccggact ggcctgcatt aactaacctt
    15301 gatattgaac tggactttat taacgacggt ttatggatga aaaccgatga cgttaatctg
    15361 ggcggcgtgc gcgcgagtaa tctcaccgca gtgatccctg actactcgaa agaaaaactg
    15421 ctgattgacg ctgacattaa aggtccgggt aaagccgttg gcccttactt tgatgagaca
    15481 ccgctgaaag attctctggg tgcgaccctg caagaactcc agctcgacgg cgatgtgaat
    15541 gctcgcttac atcttgatat cccgctgaac ggcgaactgg taaccgcgaa aggtgaagtg
    15601 acgctgcgta ataacagtct gtttatcaaa ctactcgaca gcaccctgaa aaatttgagc
    15661 ggtaaattca gctttatcaa tggcgatctg caaagtgaac cactgacagc aagctggttt
    15721 aatcagccgt tgaacgtgga tttttccacc aaagaagggg caaaagccta ccaggttgcg
    15781 gtaaacctca acggtaactg gcaaccggcg aaaaccggcg ttctgcctgc agcggtgaac
    15841 gaagcattga gtggcagcgt ggcgtgggat ggtaaagtgg gcattgatct gccttatcat
    15901 gctggcgcga cctataacgt agagctgaac ggcgatctga ataatgtgag cagtcactta
    15961 ccttcaccgt tagccaaacc tgcgggtgaa ccactgccgg taaacgttaa ggttgatggc
    16021 aatctcagca gctttgaatt aaccggacag gctggtgcgg ataaccattt caatagccgc
    16081 tggttgcttg gtcaaaagct gacgctcgat cgtgctattt gggcggcaga cagtaaaacg
    16141 ctcccgccgt tgccggaaca aagtggcgtt gaactcaata tgccgccgat gaatggtgcc
    16201 gagtggctgg ccctgttcca gaaaggcgct gcggagagtg tcggtggtgc agcgagtttc
    16261 ccacaacaca taacgttacg tacgcctatg ttgtcactgg gaaatcagca atggaataac
    16321 ctgagtattg tttcgcaacc gacggcaaat ggcaccctgg ttgaggcgca ggggcgtgaa
    16381 atcaatgcca cgctggcgat gcgtaataac gcgccgtggc tggcgaatat caaatatctt
    16441 tattacaacc cgagcgtggc gaaaactcgt ggtgattcaa caccgtcatc acctttcccg
    16501 acaacggagc gcattaactt ccgtggctgg ccggacgcac aaatacgatg cacagagtgc
    16561 tggttctggg ggcaaaaatt cggtcgtatt gacagtgatc tcaccatttc tggcgatacg
    16621 ttaacgctga ccaatggact gattgatact ggtttctcgc ggcttactgc cgatggtgaa
    16681 tgggttaata atccggggaa tgaacgtacc tcgctgaaag gaaaactgcg cgggcagaaa
    16741 attgatgccg ccgcagaatt ttttggtgtc acgacgccca tacgccagtc gtcatttaat
    16801 gtggattacg atttacactg gcgcaaagca ccctggcaac cagatgaggc gacgttgaat
    16861 ggcatcattc atactcaact gggtaaaggc gaaattaccg aaatcaatac cggacatgcc
    16921 gggcaattgc tgcgcttatt gagcgttgat gccctgatgc gtaagctgcg ttttgatttc
    16981 agagacactt ttggcgaagg gttctatttt gactccattc gcagcaccgc gtggattaaa
    17041 gacggcgtta tgcacaccga cgacacgctg gtggatggcc tggaggcgga tatcgccatg
    17101 aaagggtcgg taaatctggt acgtcgcgac ctgaatatgg aagcggttgt cgcaccagag
    17161 atttctgcga cggtgggcgt ggctgcggct tttgcggtta accccattgt tggcgcggca
    17221 gtatttgcag ccagtaaagt gctggggccg ctgtggagca aagtctccat tttgcgctat
    17281 cacatttcgg gtccgctgga cgatccgcaa atcaacgaag tgttgcgcca accgcgtaaa
    17341 gaaaaagcgc aatgatttga cgagggcgcg taattgcccc aatctcatat gataatcgtt
    17401 gccaaaggcc aacgagccag aacataaccg taggtcggat agggcgttca cgccgcatcc
    17461 ggcagccgta aaaaatcctc tactgcagta actaacgagt agcaaaaacg atgagtctta
    17521 acctagtaag tgaacaattg ctagcggcga acggcctgaa acatcaggac ttgttcgcga
    17581 tcctcggtca actggccgaa cgtcgccttg attatggcga tctctatttt cagtcgagct
    17641 atcacgaatc ctgggtttta gaagaccgca ttattaaaga tggttcttac aacatcgatc
    17701 agggcgttgg cgtgcgtgca atcagcggtg aaaaaaccgg atttgcttac gctgaccaaa
    17761 tcagcctgct ggcgctggaa cagagtgcgc aagcggcgcg caccatcgtc cgtgatagtg
    17821 gtgatggcaa agtacagacg ctgggcgcgg tagggcatag cccgttgtat acctcggtag
    17881 atccgctgca aagcatgagc cgtgaagaga agctggatat cctgcgtcgc gtcgataagg
    17941 ttgctcgcga agcggacaag cgcgtacagg aagtgactgc cagcctcagt ggtgtctatg
    18001 aattaattct ggttgcggcc accgacggca cgctagcggc ggatgtccgt ccgctggtgc
    18061 gtctttccgt gagcgttctg gtcgaagaag atggcaaacg cgaacgcggt gccagtggcg
    18121 gtggcggtcg ttttggttat gagttcttcc ttgccgatct tgacggcgaa gtccgtgcgg
    18181 atgcatgggc aaaagaagca gtgcgtatgg cgctggtcaa tctttctgcc gttgctgcac
    18241 cagcgggcac catgccggta gtacttggcg caggttggcc gggcgtgctg ttgcatgaag
    18301 cggtaggtca cggtctggaa ggcgacttca accgccgcgg cacttcagta tttagtgggc
    18361 aggtcgggga gctggtggct tcagaactgt gtaccgtggt tgatgacggc acgatggtcg
    18421 atcgccgtgg ttcggtggcg attgatgacg aaggtacgcc aggccagtac aacgtgctga
    18481 ttgagaacgg cattctgaaa ggctacatgc aggataaact caacgcgcgt ttgatgggga
    18541 tgacgccgac tggcaacggt cgccgtgaat cctacgccca tctgcccatg ccgcgtatga
    18601 ccaacaccta tatgctgccg ggtaaatcga ccccgcagga aattattgaa tccgttgagt
    18661 acggtatcta tgcaccgaac tttggtggcg gtcaggtgga tatcacctcc ggcaaattcg
    18721 ttttctccac ttcagaagca tatctgattg aaaacggtaa agtaacgaag ccggtgagag
    18781 gcgcaacgtt gattggttcc ggtatcgaaa ccatgcagca gatttcgatg gttggcaacg
    18841 acctgaaact ggataacggc gtgggtgtct gcggtaaaga agggcaaagt ttgccggttg
    18901 gcgtgggcca gccaacgctg aaagttgata acctgactgt tggcggtact gcgtaataat
    18961 ttcattattt tcagaaggat aattaatctt tctacgtgca cgaacggtcc cctcgctcct
    19021 ctggggttag ggtgagggga acccgttggc acaggtttgt agaacgtaac agtacaatat
    19081 gaattacttc tctttcccgc gcccgtgcat ctcctgaaac aatttaccga cctcaacaaa
    19141 ataatccgtc agcgagttga tcacgacctg taccttcagc ggcagcttat ctttttcggt
    19201 atataacgca taaaccgggc gtggatctga ctgataacgc ggcagcagga tctccagctc
    19261 cccacgattg atctcgttga tcacccacat cagcggcacg taggcgatcc cggcacccgc
    19321 cgttagccag cgcaccagcg tcatcggatc attagtcaca aatctccctt gcgggatcag
    19381 gcgagtcgag atcccttccg gtgcaatcag ttcaaattca ttgtcgggcc gcacgctgta
    19441 ttcaagccat gaatggctac ttaaatcggc gggtttttcc ggtatgccgt attgtgtgag
    19501 atagcttttc gcggcgcaca ccaccattgg catcgcgcca agacggcggg aaaacaggct
    19561 ggaatcctgc aacgcgccga cgcggatcac cacatccaga ccgtcggcaa tcaggtcggg
    19621 ggctggaatt ccggtaacca gattgacgct caaacctggg tattctttca gcattttggc
    19681 tgtcagcccg gcgagaacat tttgtgccat agttgaagaa cagccaatgc gtagcgtccc
    19741 gatgggggtg ttattgaaag catacagttg ctcatgaaca tcctgcactt catgaagcat
    19801 acgacggcag ccctggtagt aaattctacc ggcttcggtc aggccaatgc tgcgtgtgct
    19861 acggtttaac agctttacct gcaactcatc ttccagtttt gacaccgtct gactgatgga
    19921 cgaaacgctc atctgtagct gtctggcggc ggcggtaaaa gagccaaatt caactacttt
    19981 ggcaaacacc gacatgcgtt ttagtcgttc cattattcac tctgacttaa aagtgattta
    20041 gatcacataa ta
//
LOCUS       DAJTSL010000066        20048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTSL010000066 DAJTSL010000000
VERSION     DAJTSL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.066"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(52..1079)
                     /locus_tag="OO433_002123"
                     /pseudo
     CDS             complement(52..1079)
                     /locus_tag="OO433_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(1089..1901)
                     /gene="hcaB"
                     /locus_tag="OO433_002124"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(1089..1901)
                     /gene="hcaB"
                     /locus_tag="OO433_002124"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6050598.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(1898..2218)
                     /locus_tag="OO433_002125"
     CDS             complement(1898..2218)
                     /locus_tag="OO433_002125"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6050599.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGSDIFIDLP
                     EAQP"
     gene            complement(2218..2736)
                     /locus_tag="OO433_002126"
     CDS             complement(2218..2736)
                     /locus_tag="OO433_002126"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6050600.1"
                     /translation="MSAQVSLELHLRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRPRHLISNC
                     QISETDIPNVFAVRVNYLLYQAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(2733..4094)
                     /locus_tag="OO433_002127"
     CDS             complement(2733..4094)
                     /locus_tag="OO433_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6050601.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKSPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            4230..5120
                     /gene="hcaR"
                     /locus_tag="OO433_002128"
     CDS             4230..5120
                     /gene="hcaR"
                     /locus_tag="OO433_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6050602.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            5280..6419
                     /locus_tag="OO433_002129"
     CDS             5280..6419
                     /locus_tag="OO433_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6050603.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(6411..7691)
                     /gene="csiE"
                     /locus_tag="OO433_002130"
     CDS             complement(6411..7691)
                     /gene="csiE"
                     /locus_tag="OO433_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6050604.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(7882..8736)
                     /locus_tag="OO433_002131"
     CDS             complement(7882..8736)
                     /locus_tag="OO433_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6050605.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(8881..9684)
                     /gene="suhB"
                     /locus_tag="OO433_002132"
     CDS             complement(8881..9684)
                     /gene="suhB"
                     /locus_tag="OO433_002132"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6050606.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAVEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            9803..10543
                     /gene="trmJ"
                     /locus_tag="OO433_002133"
     CDS             9803..10543
                     /gene="trmJ"
                     /locus_tag="OO433_002133"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6050607.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10813..11301
                     /gene="iscR"
                     /locus_tag="OO433_002134"
     CDS             10813..11301
                     /gene="iscR"
                     /locus_tag="OO433_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6050608.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            11413..12627
                     /gene="iscS"
                     /locus_tag="OO433_002135"
     CDS             11413..12627
                     /gene="iscS"
                     /locus_tag="OO433_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6050609.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            12655..13041
                     /gene="iscU"
                     /locus_tag="OO433_002136"
     CDS             12655..13041
                     /gene="iscU"
                     /locus_tag="OO433_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6050610.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLVEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            13058..13381
                     /gene="iscA"
                     /locus_tag="OO433_002137"
     CDS             13058..13381
                     /gene="iscA"
                     /locus_tag="OO433_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6050611.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            13477..13992
                     /gene="hscB"
                     /locus_tag="OO433_002138"
     CDS             13477..13992
                     /gene="hscB"
                     /locus_tag="OO433_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6050612.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            14009..15859
                     /gene="hscA"
                     /locus_tag="OO433_002139"
     CDS             14009..15859
                     /gene="hscA"
                     /locus_tag="OO433_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6050613.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            15861..16196
                     /gene="fdx"
                     /locus_tag="OO433_002140"
     CDS             15861..16196
                     /gene="fdx"
                     /locus_tag="OO433_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6050614.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            16208..16408
                     /gene="iscX"
                     /locus_tag="OO433_002141"
     CDS             16208..16408
                     /gene="iscX"
                     /locus_tag="OO433_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6050615.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            16586..17869
                     /gene="pepB"
                     /locus_tag="OO433_002142"
     CDS             16586..17869
                     /gene="pepB"
                     /locus_tag="OO433_002142"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6050616.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRASERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            18011..18787
                     /gene="sseB"
                     /locus_tag="OO433_002143"
     CDS             18011..18787
                     /gene="sseB"
                     /locus_tag="OO433_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6050617.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLFIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(19280..>20048)
                     /gene="sseA"
                     /locus_tag="OO433_002144"
     CDS             complement(19280..>20048)
                     /gene="sseA"
                     /locus_tag="OO433_002144"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6050618.1"
                     /translation="RMASPGQEDRNVAQEYLNGHIPGAVFFDIEALSDHTSPLPHMLP
                     RPKTFAVAMRELGVNQDKHLIVYDEGNLFSAPRAWWMLRTFGVEKVSILGGGLAGWQR
                     DDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHENTAQIIDARPAARFNAEVDE
                     PRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFFGRGVSYDKPIIVSCGSGVTA
                     AVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT         4773 a         5115 c         5460 g         4700 t
ORIGIN      
        1 tgatgccatt ctatttcagg aaggagtgtc cgttaaactc aggctacctc agtcatcgcc
       61 acgcatatcg ccaataaact gtaagttatc actgtactga tcgctccaga accacggcag
      121 tagcggtagc ggtagcccta acattgcggc agcggcaatt tgcgcctggt tattggcgtt
      181 ttcccagctt tcacagcggt gtagtgcacc attatcaaga cgagtgattg ccacatcgcc
      241 accggcaaag atcgcgggat cgcaggtgcg gcaagcctca tcaatgacaa tgccattggc
      301 agtatcaagg ttggcctcgc gagccagttg ctcgttggcg ctgataccaa taccgtaaat
      361 caccacatca gcctgaagcg tttccccact ttgcagcgtc agttctactt tttcaccatc
      421 gaccacatgt tcaatggcat tattgagcag aatgcgcaca ccagcctgct ggtggcgctg
      481 taaaagatag cgttgcacgg gcggtggtgc attacggccc atgacggttg ccgccagttc
      541 aatcactgtc accttacatc tgcgctgcgc tgcgctgcgc tgcgctgcgt ggcgctggca
      601 gccagttcca gaccaatagt tccggcaccg acaatcacga ctgaccgttc gggctgcaga
      661 acttctcgca gtctggcggc atcaccggca tggcgtaggg taaagcagcg ttctcccagt
      721 gcatcaagca acggcagcgg tcgagctgcc gcgccggttg ctataaaaag ctgatcccag
      781 tgccagcttt cgccgttggt taacactaac tctcgtgtgt cgcgacccag cgttttgatg
      841 gttacaccgg aatgcagatg aacattgttt tcctgccacc agttagcggg taacacctgc
      901 tgtaactgcg gggaatcttc cagcaacatg gatttcgaga gtggaggtcg ttcataagga
      961 agatgtcgct catcggaaaa cagatgcagc tcaccggtga acccttgctg gcgtagcgag
     1021 gccgcagcca ttgccgccgc ttgcacgcca ccgacaataa tgatcgtttt ttctttcatc
     1081 gtgcttcctt aaagatccag cccagccgct acgtggcgaa tgccgcgaat cgctaaaccc
     1141 gcatcagcgt tgatcatcac accgcttaat gcgcgattat tgcgccgcga tgccaacatc
     1201 acatacggcc ccgtaaaatc cgccggttgc gggaaaaatt gcagcggtaa aatggcggca
     1261 attttctccg gcgtcagaga ctgcattatc gaggtttcac tttgcccgag cgcctgtggg
     1321 ccgcgcaggt cgctggccat accacacggg ccgacgccat tcacccgcac tttcggtgcc
     1381 agttcataag ccagttggcg aataagtccg gttgcggcat gtttactggc ggtgtacagc
     1441 gggccaccgc cgccaggata ccaggcggca tttgacagtg tgaaaatcat gctgccttca
     1501 ctggcgatta acgccggagc gcaggctttt gcgcccagca ggtaaccgag tacgttgacg
     1561 ttaaacagct cgtggaagcc ggtttcgagc gtctctgcgg gagtattaac cagtgaggca
     1621 ttgtgatccc agatgcctgc attgccgata aaacaatcca gcttgccgga acgagtcagg
     1681 atctgatcga ccgcgcgttg ataatcggca taacaggtca cgttaccttc caccgccaga
     1741 atatgttcgc caaatcgctg acgcagactg gcgacttttg ccgccgacag ttccagcgtg
     1801 gcaacctgcg cgccttcttc gataaatcgc tcgaccagcg ccagccctaa tcccgatccg
     1861 ccgccggtaa taaaaatgga ctcgttatgc agatcgctca tggctgcgcc tccggtaagt
     1921 cgatgaaaat gtcactacct tcaacgtgta ctggataagt ggtgagcgga tcggtggcgg
     1981 gcaggcataa cgctttcccc gttttcaggc aaaaactggc ggcgtgtagc gggcactcca
     2041 ccgtggcgtc atcttccaga tacccttctg acatcgacgc attaccatgg ctgcaacgat
     2101 cgttaatggc ataaaactcg ccgccaacgt tgaacagggc gatgacgggc gaggtatcga
     2161 tccggagagc ctcaccctcc ggcacatccg caacgggaca cgcataaatt cgattcatca
     2221 gaacagtaca ctcaggttat gggaagtgat taccgcttga tccaggacga tatcccgttc
     2281 cagcaagcgc cagttgtcat cttccagacg gcgaactttg tcgaaacgcg ttccaacata
     2341 gaatgtttca tcgcgctctt tttgtgcctg ataaagcaga taatttaccc gcacagcaaa
     2401 tacgtttggg atgtcggttt cgcttatctg gcagttgctg attaagtgac gggggcgtga
     2461 cggcggctct tctgcccagg ccatgcccgt ttccagacgg gcgattcgcc gctccagctg
     2521 gtctttggtg tcattaaaaa tccaggttgt cggtggctgg acgcctttgc ggcggtcgcg
     2581 tgtttgcgcg ttaactgtgg tgcgcatggt gtaacgaatc tcttcgtcga gctgcgccag
     2641 ccagtcacga aatttccagt cgtccagtaa gctggcttcg tgaaagagaa actggctaat
     2701 gcggagatgt aactctagtg aaacttgcgc actcatttca tcacctcctg ctggtaagcg
     2761 gcggttttat cgagcacttc ctgccagctt tcgctactca gaagatcggc ccagcgttgg
     2821 tacattccac gagcggcagt ttctgaaaag atatagttag taatgccagg aatgccgtcg
     2881 tcgcggcgct tttcctgacc aagccccatt tccagacaca gtttgctgtt gcgggcgcgg
     2941 tggcctttaa gcaatttctg gatttcacac cagttctccg agtcatcctg ctcgagaaaa
     3001 ccagcaggac caaaagcacg agtggcgctg ttttcaaaag cggctttaac ttcatcggag
     3061 gcggctttgt cagtaataca gaacgcccac acttcaactt gatcagggcc gcgcggatgc
     3121 cagacgcgga gtgtggcagt gccgtttagc catgaaagcg tggggaatat attgttatga
     3181 cccgccaggc gcagggcgcg aacttcacct aaacgttgtt ctgcttcggc ataggtttcg
     3241 cggtaatagc tggaaaccgc gccatcgacc cagacattag catccggttt ttcagtaaag
     3301 aagaaaccgc taccgtgacc gtcctgacca aattgcagcg catctttggc ggtttcccac
     3361 accgggcggg cggtttgtcc atcaccgaga cgcttatcgc tgccatcatc tttcgcccct
     3421 aatacctgaa cggcagaagc atggctgaac agagcatgat attggtcact ggcgaactgc
     3481 tctgccgggg atttccagtt acagttgatc acccactttt gtacgccgcc gacaatttcg
     3541 gtgccgcctt cgcgacgatc cagcatgcca tccagatacc aggcaatgtc accaaggtaa
     3601 tcacgcaggc ccggtgcgct ggtatcccag ttgccaaaaa tcagcccttt ataactctcc
     3661 acacaaggaa cttcgtttag cccccagtgg gatttacaca acccttgtgg gtaggcgcga
     3721 ggttccagcg gtacatcgat caactcgccg ttaatgccat aagaccagcc gtgatacggg
     3781 caggtaaagg cgcgagtgtt gccgcaatct gcataactca cacgcatggc ccggtggcgg
     3841 cattggttga gaaacgcctt gatgctgccg tctttctgac gcactacgac aaccgcatct
     3901 tctcccatgt aggtgttaaa gaaatcaccg ggttttggga tctggctttc gtgggcgaga
     3961 aatagccagc aacgaccgaa aatacgctca agctccagtt ggtaaatatc cgggtcggta
     4021 taaatacgcg gagtgacccg accattttgg gtgtcaatca gttggtaaat gttcaaatct
     4081 gagggtgtgg tcatattttt taatcctgaa agataagatg tattgacctt ctatttttgt
     4141 gccataagga tgttgctggt tggttgctaa tatgtgaaat agttaatttc taccagctga
     4201 gacgtttttt cgataacaag gggaaggtga tggaactacg gcatttacgc tatttcgtcg
     4261 cagtggcgca ggcactgaac tttacccgtg cggcggaaaa actgcatacc tcacagcctt
     4321 cgttaagcag ccagatccgc gatcttgaaa actgtgtcgg tgttccgtta ctggtgaggg
     4381 ataagcgcaa agtcgcgttg acggcggcgg gagaatgttt tctccaggac gcgctggcaa
     4441 tcctcgaaca agcggaaaat gccaaattac gggcgcggaa aattgttcag gaagacagac
     4501 aattaaccat tggctttgtg ccatcggcgg aagtgaattt actgccaaaa gtattaccga
     4561 tgtttcgtct cagacagcca gacaccttga ttgagctggt gagtttaatc accacgcaac
     4621 aggaggaaaa aattcgccgt ggtgaactcg acgtcggctt aatgcgccat cctgtttata
     4681 gcccggagat tgattatctg gagctttttg acgaaccatt agtggttgtg ttaccggttg
     4741 atcacccttt agcacatgaa aaagagatca ccgccgcgca actggatggc gtgaatttcg
     4801 tcagtaccga tccggcgtat tccggttcgc ttgcgccgat cgttaaagcg tggtttgcgc
     4861 aagaaaacag ccagccaaat atcgtccagg tggcaacgaa tattctggtg accatgaatc
     4921 tggtgggcat ggggctgggc gtcactttga tacccggtta tatgaataat tttaataccg
     4981 gacaggttgt ttttcgtccc attgccggaa atgtgccttc cattgcttta ctgatggcgt
     5041 ggaagaaagg ggagatgaag ccggcattac gcgatttcat cgccattgtg caggaacgtt
     5101 tggcaagcgt aacggcataa ttacgcatta tttttgcaac atgcgtcgcg gatcgcagtt
     5161 ttttagtgct tgtgaactgg cgttacagac atcgggaagc aggctcgaat attcttattt
     5221 ttcaacttgc accaacattc gattaacccc agactaacgc ctcctgacgg gagggactca
     5281 tggttttgca atccacgcgc tggttggcgc tcggctattt cacatacttt tttagttacg
     5341 gcatttttct acctttctgg agcgtctggc ttaaagggat tggtttaacg ccagaaacca
     5401 tcggcctgtt attgggggca ggtctggttg cccgttttct cgggagtttg ctcatcgcgc
     5461 cccgcgtcag cgatccttct cgcctgattt ccgccttgcg cgtgctggca atgatgacac
     5521 ttctctttgc tgtcgccttc tgggcggggg cgcacgtagc gtggctgatg ctggtgatga
     5581 ttggctttaa cctctttttc tcaccgctgg taccgttgac cgatgcactg gcgaatacgt
     5641 ggcaaaagca gttcccgctt gattacggca aagtgcgact gtggggctcg gtggcgtttg
     5701 tcattggctc ggcgctgacg ggcaaactgg tcactatgtt tgattatcag gtgatcctcg
     5761 cgctgttaac attgggcgtg gcatccatgc tgctcggctt tctcatccgt ccgacgattc
     5821 agccacaagg ggcaagccgc cagcaggaga gcaccggttg gtcagcgtgg ttggcgctgg
     5881 ttcttcagaa ctggcgcttt ctggcctgcg tttgtttatt gcagggggca catgcggcct
     5941 attacggttt tagcgccatt tactggcagg cagctggcta ctcggcctcg gcggtggggt
     6001 atttgtggtc gctgggcgtg gtggcggaag tcattatctt tgcgctgagt aataaacttt
     6061 tccgccgttg tagtgcacgc gatatgctgt tgatctcggc gatttgcggc gtagtgcgct
     6121 ggggcattat gggagcaact acggcgttgc cgtggttgat agtggtgcaa attctgcatt
     6181 gcggcacctt cacggtctgc cacctggccg ccatgcgcta tattgccgct cgccagggta
     6241 gcgaagtcat ccgtttacag gcggtttact ctgccgtcgc gatgggcggc agtatagcta
     6301 tcatgaccgt tttcgccggt ttcctgtatc aatatctggg ccacggcgtg ttctgggtaa
     6361 tggcgctggt ggcgcttccg gcaatgtttt tgcgcccgaa agttgttccc tcatgctgat
     6421 tcgagcattt tgcggatctg ctcctgttga tgtgttgtca gtgtcaggtc cgtatagatc
     6481 agcggtggtg aaaagagcgg taacggcatg gtataaggcg caataatcag tgccgcgccg
     6541 cgcggagcgc ctgtctgcaa aaatgccttt accgacatat gtttaatgtt gagcggcagt
     6601 aacgttagtt cgcgtaactg ctgctcaatc tgcgcttctc gctcgctatc attcccggta
     6661 agaagaataa tctgtttttc atgcagatcg ttttcctgca ttagccaggc accgaaaatc
     6721 accgcgacca gaccactttc ctcatcagaa agatggacgc cgtattcact ttcaaatccg
     6781 gccagcgccg cgcgggtggt gcgcaccagg cgtgggtaca gtctggcgaa ctcttctggc
     6841 agggtattat caataccgat ggcaaacaaa ctgcgattta acgcctgggc gaggtgggta
     6901 taaagctgat cgcataaccc ctgttcatca taaaaacgaa catttcccag ctcacgaaaa
     6961 tggtttacca gacgtttgat agactgacgc aattgtctgt ctcgctgatg cgcatcacgc
     7021 aatggatcgg gaacccgcaa catggaaaaa agtaatgcca taaacagtgg ctcatcaggc
     7081 ggtacaggct ggagcgcccg acgctgccag tggcgtccaa tttcctgcgc tacctgaaat
     7141 tcaaggcagg attccgccca gcgacgttgg agcggattaa actgaggcgt aattccagcg
     7201 tggtgttgca gcaaacaata ttgcagatac agacacagga aatgaatatc gcgcgtttca
     7261 aagcgttttt gcagccgtcg ggagcagaga ttcactaacg cttgtagatt ggtatcgtcg
     7321 taaaagttac gcgcaattcc gcgccgcttc agttcactct tcagggcggg ggtgaattgg
     7381 ctggtaataa atgacggaca cagacggaaa ccacgtcgta gccagtgaaa taaacacaaa
     7441 cgttggttaa gcactgtacc ttcaacccgg tagctaccgt cataaccagt cgtcagtgtg
     7501 agttgatgat agcgcaggat ctcccgtcct gtttcgctga tatcaagact ggcaatatca
     7561 tcatccacac cattaagctc gctgaaggtt gccatggtgg cggtcaaccc cggctgaaag
     7621 agcgtcagca agatctggca gcggcgctgg ggagccgaaa ggacagatgg tggagcaagc
     7681 gtaggcatca tctgtagatc tcccataagc ataattgata agaatagcta aagtttgctc
     7741 aggtaaatgg cgaagggaat gctaatcatc agaaatgttg gcaagcgtca cagaattttt
     7801 ctcatcttaa actgttctga ccagtggatt agcatgtctt aaggtggttt tgtgaatgat
     7861 tatcgtgcta gcgatcatgg gttagttctg cggattcaac gcgctaagga taaagtccac
     7921 catctgttcg cgataaacat cgccgtgacg atcggaaaaa gcatcaatgt gttcgccatc
     7981 cgggattagg atcaaccgtt ttgtctcttt tgccaggcta tacaactttt cgctgtgctg
     8041 ccatgggata acgtgatcag ctttaccgtg aatgagtaaa agcgggatcg ggctgacgct
     8101 ggcgatataa ttttcgccgc tgtaactctc atcaagtaag tagccactgc cggggatcat
     8161 ctgattggcg atggttgcat aagaggcaaa tgtggagtcg aggatcaccg cacgtatgcc
     8221 ttcacgatca tcctgaccaa taacagcaag aatattcgcc ccgccaatac tctgcccgaa
     8281 cagcaccagc cgttgtgggt ttacatcact gcgatggcgc accacattga tggcactttg
     8341 cgtatcgtcc agcaatccgg cctgggacgg cgtgcctttt gatttaccaa acccgcgata
     8401 atcaaacata aaaacgttga aattacgctc gggtaaccaa ctgaccagcg gccagtgggc
     8461 ggacatattt ccggcattgc cgtgagcatg aatgatggtt gcgatggcat tgtcagcagg
     8521 gcccgtcgaa gaaggaataa accagccttg cagacgagta ccgtccttag ccgtaaattc
     8581 gacggattcc gccgaccagg ggtcgggacc gtaaatttta tcgtcaggat aatagaagaa
     8641 gttgatggca acgcgcggca ctaaataaac gcagaaggca acaacaaaga gaataaacag
     8701 aattttgaga acgcgtttgt tcactggcag cgccataacc tttccttgtg acttctttat
     8761 catccattaa tcggggaaag atggtgggag agtgacgaaa gaaagtaaag cggtgaggaa
     8821 gtatttcgga atagcgcctg aaaggcgagg gcgggtgagt gacatcaccc gcctgagtca
     8881 ttaacgcttc agagcgtcgc ttaactcatc acgcatgttc gccagcatgg ctttcacaac
     8941 acgcgggtta ccagcaacga tgttaccggt cagcatatag ttatgaccac cggtgaagtc
     9001 gctgacgatg ccgcccgctt cacgaaccag cagctcgcct gccgcgaaat cccacggacg
     9061 cagaccgatt tcaaagaaac cgtcaacacg acccgcagcg acgtaagcca gatccagcgc
     9121 cgcagaaccg gtacgacgga agtctgcaca ttcgttgaac agtttgccga cgatgttgat
     9181 gtaggtagtg gcgtactgtt ttgctttgaa cgggaagccg gtcgccagaa tggtaccgtc
     9241 gagatcgcga gcggtgctac cgcgcagacg gtagccgttc agctgtgcgc cctgaccgcg
     9301 agtggcggtg aacagttcgt tacgcatagg atcgtatacc acagcaactt cggtgcggcc
     9361 tttgatacga acggcgatag ataccgcgaa gtgcggcaga cgtttgataa agttggtagt
     9421 accatccagt ggatcgataa cccattgaac atcctgatca gtaccttcaa gttcaccgct
     9481 ttcttcggtg atgatggtgt gctgtgggta agatttacga atcgtgtcga taatcaccgc
     9541 ttcgacagct ttatctacgt tggtcacgaa atcgttactg cctttctggc tcgcttctac
     9601 agcgtccggg gtttcatagt ttttggcaat taaattaccc gccttgcgcg ctgcgcgcac
     9661 ggcgatgttc agcatcggat gcatcggtct ctctcactgg atgttaaaga acgggaaaac
     9721 ggcgcagagt atagcagcga gatcggcatc tgtcttcgtt ttatgataag atgcgtgaat
     9781 aatctttaga tgaagaaaga caatgctgca aaatattcga attgtgctgg tggagacgtc
     9841 acacaccggc aatatgggtt ctgttgcccg tgccatgaaa acaatgggat taaccaatct
     9901 gtggctggtt aatccactgg tgaaacccga ctcccaggcg attgccctgg cagcaggggc
     9961 cagcgatgtg attggtaatg ctcacatcgt cgatacgctc gacgaagcgt tagccggttg
    10021 tagcttggtg gtgggcacca gcgcacgttc ccgcacgctg ccatggccga tgctcgaccc
    10081 gcgcgaatgc ggcctgaaaa gcgtcgctga agcggcaaat accccggtgg cgctggtgtt
    10141 tggtcgcgag cgcgtcggct tgaccaatga agagttgcag aaatgccatt atcatgtcgc
    10201 gattgcggct aacccggaat acagctcgct gaacctggcg atggcggttc aggttattgc
    10261 ctatgaagtg cgcatggcct ggctggcaac tcaggaaaac ggcgagcagg tcgagcatga
    10321 agagacgccg tatccgctgg tcgatgatct ggagcgtttt tatggtcatc tggaacaaac
    10381 gctgctggca accggtttta tccgtgaaaa ccatccgggg caggtgatga ataaattgcg
    10441 ccgtctgttt acccgtgcgc gcccggaaag ccaggaattg aatatcctgc gcgggattct
    10501 ggcttctatt gagcagcaga ataaaggtaa caaggccgaa taacagtagt tgcctgatgc
    10561 gacgcttaac gcgtcttatc aggcctacaa tgtacagaac cgtaggtcgg ataaggtgtt
    10621 cacaccgcat ccgacaacag gtacaaacgc cacgataaaa aaatggcact gaaggttaaa
    10681 tacccgacta aatcagtcaa gtaaatagtt gaccaattta ctcgggaatg tcagacttga
    10741 ccctgctatt caataccccc acttttacaa taaaaaaccc cgggcagggg cgagtttgag
    10801 gtgaagtaag acatgagact gacatctaaa gggcgctatg ccgtgaccgc aatgcttgac
    10861 gttgcgctca actctgaagc gggcccggta ccgttggctg atatttccga acgtcagggc
    10921 atttcccttt cttatctgga acaactgttt tcccgtctgc gtaaaaatgg tctggtttcc
    10981 agcgtacgtg gaccaggcgg tggttatctg ttaggcaaag atgccagcag catcgccgtt
    11041 ggcgaagtga ttagcgccgt tgacgaatct gtagatgcca cccgttgtca gggtaaaggc
    11101 ggctgccagg gcggcgataa atgcctgacc cacgcgctgt ggcgtgattt gagcgaccgt
    11161 ctcaccggtt ttctcaacaa cattacttta ggcgaactgg ttaataacca ggaagtgctg
    11221 gatgtgtctg gtcgtcagca tactcacgac gcgccacgca cccgcacaca agacgcgatc
    11281 gacgttaagt tacgcgctta ataaaaagaa ttcagaatca ggccggagtg ctaaatactc
    11341 cgtaaacacg gtcgtacatc cagccggtag cctgattcct tgcattgagt gatgtacgga
    11401 gtttatagag caatgaaatt accgatttat ctcgactact ccgcaaccac gccggtggac
    11461 ccgcgtgttg ccgagaaaat gatgcagttc atgacgatgg acggaacctt tggtaacccg
    11521 gcctctcgtt ctcaccgttt cggctggcag gctgaagaag cggtagatat cgcccgtaat
    11581 cagattgccg atctggtcgg cgctgatccg cgtgaaatcg tctttacctc tggtgcaacc
    11641 gaatctgaca acctggcgat caaaggtgca gccaactttt atcagaaaaa aggcaagcac
    11701 atcatcacta gcaaaaccga acacaaagcg gtactggata cctgccgtca gctggagcgc
    11761 gaaggttttg aagtcaccta cctggcaccg cagcgtaacg gcattatcga cctgaaagaa
    11821 cttgaagcag cgatgcgtga cgacaccatc cttgtttcca tcatgcacgt aaataacgaa
    11881 atcggcgtgg tgcaggatat cgcggctatc ggcgaaatgt gccgtgctcg tggcattatc
    11941 tatcacgttg atgcaaccca gagcgtgggc aaactgccta tcgacctgag ccagttgaaa
    12001 gttgacctga tgtctttctc cggtcacaaa atctatggcc cgaaaggtat cggtgcgctg
    12061 tatgtacgtc gtaaaccgcg cgtacgcatc gaagcgcaaa tgcacggcgg cggtcacgag
    12121 cgcggtatgc gttccggcac tctgcctgtt caccagatcg tcggaatggg cgaggcctat
    12181 cgcatcgcaa aagaagagat ggcgaccgag atggaacgtc tgcgcggcct gcgtaaccgt
    12241 ctgtggaacg gcatcaaaga tatcgaagaa gtttacctga acggtgacct ggaacacggt
    12301 gcgccgaaca ttctcaacgt cagcttcaac tacgttgaag gtgagtcgct gattatggcg
    12361 ctgaaagacc tcgcagtttc ttcaggttcc gcctgtacgt cagcaagcct cgaaccgtcc
    12421 tacgtgctgc gcgcgctggg gctgaacgac gagctggcac atagctctat ccgtttctct
    12481 ttaggtcgtt ttactactga agaagagatc gactacacca tcgagttagt tcgtaaatcc
    12541 atcggtcgtc tgcgtgacct ttctccgctg tgggaaatgt acaagcaggg cgtggatctg
    12601 aacagcatcg aatgggctca tcattaatcg gtatcggaat caggagaatt tataatggct
    12661 tacagcgaaa aagttatcga ccattacgag aatccgcgta acgtgggttc ctttgacaac
    12721 aacgacgaga acgtcggcag cggcatggtg ggtgcaccgg cctgtggcga cgtgatgaag
    12781 ttgcagatta aagtcaacga tgaaggtatc attgaagacg cgcgttttaa aacttacggc
    12841 tgcggttctg ctatcgcttc cagctccctg gtcaccgaat gggtgaaagg gaagtctctc
    12901 gtcgaagcgc aggcgatcaa aaacaccgat attgctgaag aacttgaact gccgccggtg
    12961 aaaattcact gttctattct ggcagaagac gcgatcaaag ccgccattgc ggactataaa
    13021 agcaaacgtg aagcaaaata agagttgagg tttggttatg tcgattacac tgagcgacag
    13081 tgcagcagcg cgagtaaata cctttctggc taaccgcggc aaagggtttg gcctgcgtct
    13141 gggcgtgaga acctccggat gttcaggtat ggcttatgta ctggaatttg ttgacgaacc
    13201 gacgccggaa gacatcgtgt ttgaagacaa aggcgtgaaa gtcgtggtcg atggcaaaag
    13261 cctgcaattt ctggacggta cgcagctgga cttcgtaaaa gaaggcctga atgaagggtt
    13321 taaattcacc aacccgaacg ttaaagatga gtgtggttgc ggcgaaagct tccacgtttg
    13381 atgcgcatac cgataacccc accgtggtcg cctgcgcgtg gggtttgttt tacctgattc
    13441 gccgttatcg cggcggatcg cagccctgag aatgttatgg attacttcac cctctttggc
    13501 ttgcctgccc gttatcaact cgatacccag gcactgagcc tgcgttttca ggatctacaa
    13561 cgtcagtatc atcctgataa attcgccagc ggaagccagg cggaacaact cgccgccgta
    13621 cagcaatctg caaccattaa ccaggcctgg caaacgctgc gtcatccgtt aatgcgcgcg
    13681 gaatatttgc tttctttgca cggttttgat ctcgccagcg agcagcatac tgtgcgcgac
    13741 accgcgttcc tgatggaaca gctggagctg cgcgaagaac tggacgagat cgaacaggcg
    13801 aaagatgaag cgcggctgga aagctttatc aaacgtgtga aaaagatgtt tgatacccgc
    13861 catcagttga tggttgaaca gttagacaac gagacgtggg acgcggcggc tgataccgtg
    13921 cgtaagctgc gttttctcga taaactgcga agcagtgccg aacaactcga agaaaaactg
    13981 ctcgattttt aatttctgga agctaaacat ggccttatta caaattagtg aacctggttt
    14041 gagtgctgcg ccgcatcagc gtcgtctggc ggccggtatt gacctgggca caaccaactc
    14101 gctggtagcg acagtgcgca gcggtcaggc cgaaacgtta gccgaccatg aaggccgtca
    14161 cctgctgcca tctgttgttc actatcaaca gcaagggcat tcggtgggtt atgacgcgcg
    14221 tactaacgca gcgctcgata ccgccaacac cattagttct gttaaacgcc tgatgggacg
    14281 ctcgctggct gatatccagc aacgctatcc gcatttgcct tatcaattcc aggccagcga
    14341 aaacggcctg ccaatgattg aaacggcggc ggggctgctg aacccggtgc gcgtttctgc
    14401 ggacatcctc aaagcactgg cggcgcgggc aactgaagcc ctggcaggcg agctggatgg
    14461 tgtcgttatc accgttccgg cgtactttga cgatgcccag cgtcagggca ccaaagacgc
    14521 ggcgcgtctg gcggggttgc atgttctgcg cttacttaac gaaccgaccg ctgcggctat
    14581 cgcctacggg ctggattccg gtcaggaagg cgtgatcgcc gtttatgacc tcggtggcgg
    14641 gacgtttgat atttccattc tgcgcttaag tcgcggcgtg tttgaagtgc tggcaaccgg
    14701 cggtgattcc gcgctcggcg gcgatgattt cgaccatctg ctggcggatt acattcgcga
    14761 gcaggcggac attcctgatc gtagcgataa ccgcgttcag cgtgaactgc tggatgccgc
    14821 cattgcagcc aaaatcgcgc tgagcgatgc ggactccgtg accgttaacg ttgcgggctg
    14881 gcagggcgaa atcagccgtg aacaattcaa tgaactgatc gcgccactgg taaaacgaac
    14941 cttactggct tgtcgtcgtg cgctgaaaga cgcgggcgta gaagctgatg aagtgctgga
    15001 agtggtgatg gttggcggtt ctactcgcgt gccgctggtg cgtgaacggg taggcgaatt
    15061 tttcggtcgt ccaccgctga cttccatcga cccggataaa gtcgtcgcta ttggcgcggc
    15121 gattcaggcg gatattctgg tgggtaacaa gccggacagc gaaatgctgc tgcttgatgt
    15181 gatcccactg tcgctgggcc tcgaaacgat gggcggcctg gtggagaaag tgattccgcg
    15241 taataccact attccggtgg cccgcgctca ggatttcacc acctttaaag atggtcagac
    15301 ggcgatgtct atccatgtaa tgcagggtga gcgcgaactg gtgcaggact gccgctcact
    15361 ggcgcgtttt gcgctgcgtg gtattccggc gctaccggct ggcggtgcgc atattcgcgt
    15421 gacgttccag gtcgatgccg acggtctttt gagcgtgacg gcgatggaga aatccaccgg
    15481 cgttgaggcg tctattcagg tcaaaccttc ttacggtctg accgatagcg aaatcgcttc
    15541 gatgatcaaa gattcaatga gctatgccga gcaggacgta aaagcccgta tgctggcaga
    15601 acaaaaagta gaagcggcgc gtgtgctgga aagtctgcac ggcgcgctgg ctgctgatgc
    15661 cgcgctgtta agcgccgcag agcgtcaggt cattgacaat gctgccgctc acctgagtga
    15721 agtggctcag ggcgatgatg ttgacgccat cgaacaagcg attaaaaacg tagacaaaca
    15781 aacccaggat ttcgccgctc gccgcatgga ccagtcggtt cgtcgtgcgc tgaaaggcca
    15841 ttccgtggac gaggtttaat atgccaaaga ttgttatttt gcctcatcag gacctctgcc
    15901 ctgatggcgc tgttctggaa gctaatagcg gtgaaaccat tctcgacgca gcgctgcgta
    15961 acggtatcga gattgaacac gcctgtgaaa aatcctgtgc ttgcaccacc tgccactgca
    16021 tcgttcgtga aggttttgac tcgctgccag aaagctcaga gcaggaagac gacatgctgg
    16081 acaaagcctg gggactggag cccgaaagcc gtttaagctg ccaggcacgc gttaccgacg
    16141 aagatttagt agtcgaaatc ccgcgttaca ctatcaacca tgcgcgtgag cattaacaga
    16201 ggttagtatg ggacttaagt ggaccgatag ccgcgaaatt ggcgaagcac tgtacgatgc
    16261 gtatcctgat cttgatccga aaacggttcg attcaccgat atgcatcagt ggatttgcga
    16321 tctggaagat ttcgacgacg acccgcaggc atccaacgag aaaatcctcg aagcgatttt
    16381 gttagtctgg ctggacgagg ccgaataatt tccgcgtcat gcttcacgcc gcagatgcgt
    16441 tggctgcgct cgttcacccc agtcacatag ttatctatgc tcctggggct tcactcactt
    16501 gccgccttcc tgcaacgcga atcatttagc ggaaaaatcc tggggctgcc aactggcggc
    16561 ccttttacaa agaaggataa ctaaaatgac agaagcgatg aagattaccc tctctaccca
    16621 acctgccgac gcgcgctggg gagaaaaagc aacttacagc attaataatg acggcattac
    16681 cctgcatttg aacggggcag acgatctggg gctgatccag cgtgcggcgc gcaagattga
    16741 cggtctgggc atcaagcatg ttcagttaag cggtgaaggc tgggatgcgg atcgctgctg
    16801 ggcattctgg caaggttaca aagccccgaa aggcacgcgt aaagtggagt ggccggatct
    16861 ggacgatgcc cagcgccagg aactggataa ccgcctgatg atcatcgact gggtgcgtga
    16921 caccatcaac gcaccggcag aagaattggg accatcgcaa ctggcacagc gtgctgttga
    16981 tctgatcagc aacgtcgcgg gcgatcgtgt gacttatcgg atcaccaaag gcgaagatct
    17041 gcgtgagcaa ggttatatgg ggctgcacac cgtcggacgc gcttcagaac gttctccggt
    17101 attgctggcg ctggattaca acccaactgg cgataaagaa gcgccagtgt acgcgtgcct
    17161 ggtaggtaaa ggtatcactt ttgactccgg cggctacagc atcaaacaga ctgcgtttat
    17221 ggactcgatg aagtcggaca tgggcggcgc ggcaacggtt accggggcgc tggcatttgc
    17281 cattacgcgc ggactgaaca agcgcgtgaa gctgttcctc tgctgtgcgg ataacctgat
    17341 tagcggcaat gcgttcaagc tgggcgatat catcacctat cgcaacggta aaaaagttga
    17401 agtgatgaac actgatgccg aagggcgtct ggtgcttgcc gatggtctga ttgatgccag
    17461 tgcgcagaaa ccggaaatga tcattgatgc ggcgaccctc accggggcgg cgaaaactgc
    17521 gctgggtaat gattatcacg cgctgttcag ttttgacgat gcgcttgccg gtcgtttgct
    17581 ggcgagtgcc tcacaagaga acgaaccatt ctggcgtctg ccgctggcgg aattccaccg
    17641 cagccagctg ccgtctaact ttgccgaact gaacaatacc ggaagcgcgg cgtatccggc
    17701 aggcgcgagc acggcggcgg gcttcctgtc gcactttgtt gagaactatc agcaaggctg
    17761 gctgcatatc gactgctcgg cgacttaccg taaagcgccg gttgaacagt ggtctgcggg
    17821 cgctacggga cttggtgtgc gcacgatagc taatctgtta acggcgtaaa ctaatatgcc
    17881 ggatgcggcg taaacgcctt atccggccta caaattctgc aaattcaaca aattgcgaat
    17941 cccttgtagg cctgataagc gtagcgcatc aggcatttgc gccccgaacg ttgaatgaat
    18001 tgtggttctt atgtccgaaa caaaaaacga acttgaagac ctgctggaaa aagcagcaac
    18061 cgaaccggca caccgcccgg cctttttccg tactctactg gaatccaccg tctgggtgcc
    18121 tggtacggcg gcgcagggcg aggctgtggt tgaagatagt gcgcttgatt tacagcactg
    18181 ggaaaaagaa gacggcacca gcgtcattcc ttttttcacc tcgttagaag cgcttcaaca
    18241 ggcggttgaa gacgaacagg catttgtcgt aatgcccgtt cgcacattgt ttgagatgac
    18301 ccttggcgaa acgctcttcc ttaatgccaa actgccgacc ggtaaagaat ttatgccgcg
    18361 tgaaatcagt ttgttgattg gtgaagaggg aaatccgctg agcagccagg aaatcctgga
    18421 aggcggtgaa tcgctgatat tatcggaagt tgcagagccg ccagcacaaa tgattgattc
    18481 actcaccacc ttatttaaaa ccattaagcc ggtgaagcgt gcttttattt gttcaattaa
    18541 agagaacgaa gaggcacagc ctaatttatt tattggcatt gaagccgatg gtgatatcga
    18601 agaaattatt caggcgacgg gaagtgtagc gaccgataca ttacctggcg atgaaccaat
    18661 cgatatttgt caggtgaaaa aaggggaaaa aggaattagc cactttatta ccgaacatat
    18721 tgtgccattc tatgaacgtc gctggggagg ttttttgcgt gactttaaac aaaaccggat
    18781 aatctaaaat atgccgccct aaagggcggc attcgggctt acttgctgct ctgaaggttg
    18841 ttaaccacca gtaaattgtc gccggagaga ctccggttat tatttacttg ggaaaacagc
    18901 gcaccagaaa caataagtac gatacagaag caccgtatct tcattgtgcg gtccatgcga
    18961 agaccgcatc acgacagcac cactggcgat attgccgtta gagcaagcgc tgagacgcgc
    19021 ttgctctaac ggtcgcggaa catcctttcc ttaagccagc gcccgcttgc gagacggcgg
    19081 aaagggccgc agtggataat atactaacag aacaataata actctctttt gcttgacaaa
    19141 aagagagtta ctggcgagta ttgtctccgc tgagacgcgc ttgcattact acggtaatac
    19201 aacctgctgg aaaggccgcg aaccagacca gcaataaaaa accgccaaat tcggcggttt
    19261 tttattgcct gtaaaatact tatttcactg gctcaaccgg taaatctgcc cgcgcgcccc
    19321 attcactcca tgcgccgtcg tacagtttca cgtttggcac atccagcgtc gcgagtgcta
    19381 acaaaaccac ggctgccgtt acaccagagc cgcagctgac gataattggt ttgtcgtagc
    19441 tgacgccgcg accaaaaaat atcgcatcca gttcatcggt cgtttttagt tcgccttcgc
    19501 gcaccagttc cgtccacggc acattcagtg ctccgggaat atgtccgcga cgtaaacctg
    19561 ggcgaggttc atcaacttct gcgttaaaac gtgcagccgg gcgggcatca ataatttgcg
    19621 ccgtattttc atggcttgcc aataatacat cggttacttt caccacggct tcaggattaa
    19681 acgcggcgtt aaactctcct tccggcagct ctactgcacc ttcttctaac agcagatcat
    19741 cgcgctgcca gcctgcaagt ccacccccca gaatcgacac tttctctaca ccaaaggtgc
    19801 gcagcatcca ccatgctcgt ggggctgaga aaagattacc ttcgtcatag acaatcaggt
    19861 gcttatcctg gttaacgcct aattcacgca tcgccacggc gaacgttttc gggcgcggca
    19921 gcatgtgcgg aagcggggaa gtgtgatcag aaagcgcttc gatatcaaaa aacactgcgc
    19981 cgggaatatg tccattcaga tactcctgag caacgttacg atcttcctgt ccaggcgacg
    20041 ccatgcgg
//
LOCUS       DAJTSL010000067        19865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTSL010000067 DAJTSL010000000
VERSION     DAJTSL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.067"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            54..830
                     /gene="eutC"
                     /locus_tag="OO433_002145"
                     /pseudo
     CDS             54..830
                     /gene="eutC"
                     /locus_tag="OO433_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200160.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
     gene            840..1499
                     /gene="eutL"
                     /locus_tag="OO433_002146"
     CDS             840..1499
                     /gene="eutL"
                     /locus_tag="OO433_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6050619.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            1512..2012
                     /gene="eutK"
                     /locus_tag="OO433_002147"
     CDS             1512..2012
                     /gene="eutK"
                     /locus_tag="OO433_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6050620.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAAGRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            2058..3110
                     /gene="eutR"
                     /locus_tag="OO433_002148"
     CDS             2058..3110
                     /gene="eutR"
                     /locus_tag="OO433_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR6050621.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFVTDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="OO433_002149"
     CDS             complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="OO433_002149"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6050622.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCNDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="OO433_002150"
     CDS             complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="OO433_002150"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6050623.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            5102..5527
                     /locus_tag="OO433_002151"
     CDS             5102..5527
                     /locus_tag="OO433_002151"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6050624.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADMLSLGKRLIEDEEY"
     gene            5514..5963
                     /locus_tag="OO433_002152"
     CDS             5514..5963
                     /locus_tag="OO433_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6050625.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFIIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            6170..6745
                     /locus_tag="OO433_002153"
     CDS             6170..6745
                     /locus_tag="OO433_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6050626.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFRGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            6841..7740
                     /gene="yfeX"
                     /locus_tag="OO433_002154"
     CDS             6841..7740
                     /gene="yfeX"
                     /locus_tag="OO433_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6050627.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="OO433_002155"
                     /pseudo
     CDS             complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="OO433_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
     gene            complement(9107..10531)
                     /gene="murP"
                     /locus_tag="OO433_002156"
     CDS             complement(9107..10531)
                     /gene="murP"
                     /locus_tag="OO433_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6050628.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKSVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRSQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="OO433_002157"
     CDS             complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="OO433_002157"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6050629.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            11595..12452
                     /gene="murR"
                     /locus_tag="OO433_002158"
     CDS             11595..12452
                     /gene="murR"
                     /locus_tag="OO433_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6050630.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNTTALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCVEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            12581..13372
                     /gene="ucpA"
                     /locus_tag="OO433_002159"
     CDS             12581..13372
                     /gene="ucpA"
                     /locus_tag="OO433_002159"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6050631.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            13676..14692
                     /gene="cysP"
                     /locus_tag="OO433_002160"
     CDS             13676..14692
                     /gene="cysP"
                     /locus_tag="OO433_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6050632.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKTDVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            14692..15525
                     /gene="cysT"
                     /locus_tag="OO433_002161"
     CDS             14692..15525
                     /gene="cysT"
                     /locus_tag="OO433_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6050633.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            15525..16400
                     /gene="cysW"
                     /locus_tag="OO433_002162"
     CDS             15525..16400
                     /gene="cysW"
                     /locus_tag="OO433_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6050634.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            16390..17487
                     /gene="cysA"
                     /locus_tag="OO433_002163"
     CDS             16390..17487
                     /gene="cysA"
                     /locus_tag="OO433_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6050635.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            17621..18532
                     /gene="cysM"
                     /locus_tag="OO433_002164"
     CDS             17621..18532
                     /gene="cysM"
                     /locus_tag="OO433_002164"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6050636.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(18535..18903)
                     /locus_tag="OO433_002165"
     CDS             complement(18535..18903)
                     /locus_tag="OO433_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6050637.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLSQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            19008..19859
                     /gene="pdxK"
                     /locus_tag="OO433_002166"
                     /gene_synonym="thiJ"
     CDS             19008..19859
                     /gene="pdxK"
                     /locus_tag="OO433_002166"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6050638.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         4571 a         5040 c         5605 g         4649 t
ORIGIN      
        1 cggcccccgc gctgtcagaa gcaaagtgca ccaccaccac ctgtgcggca ccggtgacct
       61 cggaaagctg agcgctggat ttaggttctg cggaagcaaa agcgtggatt ggtgttgaaa
      121 acccgcatcg cgcagacgta ttaacagaac tgcgtcgcag caccgtggcc cgcgtttgta
      181 ccggtcgtgc cggtccgcgt ccgcgtaccc aggcgttgct gcgtttcctg gccgatcact
      241 ctcgttcgaa agataccgta ctgaaagaag tgccggaaga gtgggtgaaa gcgcagggct
      301 tgctggaagt acgctccgaa attagcgaca aaaatctcta cctgactcgc ccggatatgg
      361 gccgccgcct gtgtgcagaa gctgttgaag cgctgaaagc acagtgtgtt gctaatccgg
      421 acgtacaggt cgttatttct gatggcctgt caactgatgc catcaccgtg aactacgaag
      481 agctcctgcc gccgctgatg gcgggcctga aacaggccgg gctgaaagtc ggcacgccat
      541 tctttgttcg ttatggtcgc gtgaagattg aagatcagat ctgtgagatc ctcggcgcga
      601 aagtggtgat cctgctggtg ggggaacgtc cggggttggg tcagtcagaa agcctctcct
      661 gttacgccgt ttactcgccg cgtatggcga ccaccgttga ggccgatcgc acctgcatct
      721 ctaacattca ccagggcggc acgccgccgg ttgaagccgc agccgtcatt gtggatttgg
      781 cgaaacgtat gctggagcag aaagcatccg gcatcaacat gacccgataa ggaggcatca
      841 tgccagcttt agatttgatt cgaccgtcgg taaccgccat gcgggtgatt gcctccgtta
      901 acgctgaatt tgcgcgtgaa ctaaaattgc cgccgcatat tcgtagcctc gggctgattt
      961 ctgctgattc cgatgacgtc acctacattg cggctgacga agcgaccaag caggcgatgg
     1021 tcgaagtggt atatggccgc tcgctgtatg cgggagcagc acacggaccg tcaccgactg
     1081 ccggtgaagt gctgattatg cttggtgggc caaacccggc ggaagtgcgt gctggtctgg
     1141 atgcgatggt tgcgcatatt gaaaatggcg ctgctttcca gtgggctaat gacgcagaaa
     1201 acaccgcatt cctggcacat gtagtttcgc gtaccggttc ttatctctca tcaaccgccg
     1261 ggatcacgct tggcgatccg atggcgtatc tggtggcacc gccgctggaa gcgacctacg
     1321 gcattgatgc agcgttgaaa tctgccgacg tacagctggt gacctatgtc ccgccaccgt
     1381 ctgaaaccaa ctactcggca gcatttttaa ccggtagcca ggccgcgtgt aaagcagcct
     1441 gtaacgcctt taccgatgca gtgctggaaa tcgcgcgtaa tccaatccag cgtgcgtaac
     1501 ggaggttgcc gatgatcaat gcactgggat tgctggaagt ggacggcatg gtcgccgcga
     1561 tagatgcagc ggatgccatg ctcaaagcag ctaacgttcg tctgctcagt cacgaagtgc
     1621 ttgaccctgg tcgcttaacg ctggtggtgg aaggcgatct ggcggcgggt cgtgcggcgc
     1681 tggacgcagg ttgtgctgcc gcgatgcgta ccgggcgtgt catcagccgc aaggagatcg
     1741 gtcggccaga cgatgacacc cagtggctgg ttactggctt taaccgccag ccgaagcaac
     1801 ccgtaaggga acccgacgcg ccagttatcg tcgcggaatc tgctgacgag ttgttggcgc
     1861 tgttaacatc agtacgtcag ggaatgacgg caggagaagt ggctgcccac tttggctggc
     1921 cgctggaaaa agccagaaat gcgctcgaac agctcttttc tgccgggacg ttacgtaaac
     1981 gcagtagtcg ttatcgtctc aagccccatt aacctgtcgg aggtgctggg tgtcctacaa
     2041 cacccggcat taacatcatg aaaaagaccc gtacagccaa tttgcaccat ctttatcatg
     2101 aacccttacc cgaaaacctg aagctcacgc cgaaggtcga agtggataat gttcatcaac
     2161 gacagacaac ggatgtctat gaacatgctt taacgattac cgcctggcag cagatttacg
     2221 atcagctgca tccgggcaag tttcatggtg aatttacgga aattctactc gatgatattc
     2281 aggtttttcg tgaatacacc ggtctggcgc tgcgtcagtc gtgcctggtc tggccgaact
     2341 cgttctggtt tggcattccg gcgacgcgcg gtgagcaggg atttatcggt tcgcaatgtc
     2401 tgggaagcgc ggaaatcgcc acccgccctg gtggcactga atttgaactg agcacgccgg
     2461 atgattacac gatcctgggc gtggtgcttt ctgaagatgt catcacccgg caggctaact
     2521 ttttgcataa cccggatcgg gtattacata tgctgcgtag ccagtcggcg ctggaagtga
     2581 aagagcagca taaagccgcg ctgtggggct ttgtccaaca ggcgctggcg acgttttgcg
     2641 agaacccgga aaatctccat cagcctgcag tgcgaaaagt gctgggggat aatttgctaa
     2701 tggcgatggg ggcgatgctg gaagacgcgc aaccaatggt gacggcggaa agcatcagtc
     2761 atcagagtta ccgtagatta ctttcccgcg cccgtgaata tgtgctggaa aatatgtctg
     2821 agccggtgac ggtgctggac ttgtgtaatc aactgcatgt cagtcgccgc acgctacaaa
     2881 acgcgtttca cgctatttta ggcattggcc caaacgcgtg gctgaaacgc attcgcctga
     2941 acgccgtacg ccgcgaactg ataagcccgt ggtcgcaaag cacaacggta aaagacgccg
     3001 ccatgcagtg gggattctgg catctggggc aatttgtcac ggattaccag cagctgtttg
     3061 ccgagaagcc gtcgttgacg ttgcatcagc ggatgcggga atgggggtga gggagttaca
     3121 cccaatccct gaccttaata aactcactta acgccgcttc tgggctgcca tcttttggct
     3181 gataatcata ctcccagcgt accagtggcg gcattgacat caggatcgac tcggtgcgcc
     3241 cgccagtttg caggccaaac agagtgccgc gatcccagac cagattgaac tcgacataac
     3301 gaccgcgacg gtagagctga aaattgcgct cgcgctcgcc gtaggccatt gctttacgtc
     3361 gctctacaat tggtaaataa gcgtcggtgt agcctttgcc taccgcctgc ataaaggcaa
     3421 aacagtggtc gaaatctggc gtgttcagat catcaaagaa cagcccgcca ataccgcgct
     3481 gttcgttgcg atgtttgagg tagaagtaat cgttgcacca ctttttgtaa cggggataaa
     3541 cgtcttcacc aaatggcagg cacaggtcac gggcggtgcg gtgccagtga atggcgtctt
     3601 cttcaaaacc atagaaaggg gttaaatcga agccgccgcc aaaccaccag acgggctctg
     3661 cacccggttt ttcggcaata aaaaaacgca cattcgcgtg gctggtggga acatacgggt
     3721 tatgcggatg cactaccagt gaaacgccca tcgcctcgaa actgcgcccg gcaagttccg
     3781 ggcgatgagc ggtggcggaa gcaggcatcg cctcgccgtg gacgtgagaa aagttgacgc
     3841 ctgcctgttc gaaaacacca ccattacgca acacccgact acgcccgccg ccgccagctt
     3901 cgcgctgcca actatcttcg acaaattctg cgccatcgac ggcggtcagc tgctgacaaa
     3961 tcgtatcctg aaggttgagc agaaactgtt taacctggtg tgcgtcgggt ttcataactt
     4021 atcgcttttt cgaatgtgct ttctggttgt cgaaccagtg gaaataactg atcacgcctt
     4081 cagcaatcgc tgtggcgatt ttctgacgaa acgccgccgt gcctaacagc cgctcttctt
     4141 ccgggttggt gataaacgag gtttccacca gcaccgaagg aaccgacggc gatttcaaca
     4201 ccacaaacgc ggcttgttcg gtgttgcggc tgtgcagttt atgcaccggc ttaatctttt
     4261 tcagaatatg cgagccaagc gtcaggctgt ttttaatagt atcggtttgc accagatcaa
     4321 acagcacttg ctgcaatagg tgatccttgt cagtcgcctt tttaccggca acttcatcgg
     4381 cgcggttttc gcgttcagac aggtatttcg ccattgcgct acttgcccca cggttagaga
     4441 gggcaaatac cgaagcaccg gcagctttcg ggttggtaaa gccatcggca tgaattgaca
     4501 taaacagatc tgcgccatgt ttatgggcga tttcaacgcg atcgtaaagt gggataaacg
     4561 tatcgccaga acgcgttaaa cgcgcatcaa tcccatgatt acgcaaaatg gaacggacgt
     4621 ttttagcaat cgccagcacc acatgttttt ctttcgaacc gttgcgtccg atcgctccgg
     4681 tatcaattcc gccgtggcct ggatcgagaa caacgacacg tttgccgcca gattttttgg
     4741 ctttcggctt gctgtgtccg ttgctggttt ttaaaggttc gtctttggcg atggcttgcg
     4801 acattcctga caacgtcagg gcagccaaac cggctttcag cacctggcgg cgcgaagtga
     4861 gtgtttttag tggtttaaaa gtgctcatac ggcctgagtt gtaataataa agttccagat
     4921 gttatatcgt atcgcgtaat ccgttacgac ggtttgatgt tgagaattgt tttacttttc
     4981 atttcaatag ttgacaactc cccattatgc cactttttca accgattttc gtcagatatt
     5041 ggctaattct accgttcacg ccataatcag ccttttaacc cgcaaaaagg taacgaatac
     5101 catggagata cgcgtatttc gccaggaaga tttcgaagag gtcatcaccc tttgggagcg
     5161 ttgcgacttg ctgcgtccgt ggaacgatcc ggaaatggac atcgagcgta agatgaacca
     5221 tgacgtcagt ttgtttttgg tcgctgaggt gaacggtgaa gtggtcggaa cggtgatggg
     5281 cggttatgac gggcatcgcg ggtctgctta ttatcttggc gtgcatccag agtttcgtgg
     5341 gcgtgggatt gccaatgcgt tgcttaatcg gctggagaaa aagctgattg ctcgtggctg
     5401 cccgaaaatt cagatcaatg tgccggaaga taacgacatg gtgctcggaa tgtatgaacg
     5461 cctgggatat gaacacgccg acatgctgag tctgggtaag cgtttgattg aagatgaaga
     5521 gtactgagtt ccatcctgtc cattatgatg cgcacggacg cctgcgttta cccttgctct
     5581 tctggcttgt gctattgctt caggcgcgaa cctgggtgct gtttatcatt gccggtgcgt
     5641 cgcgcgagca gggtactgcg ctgctaaatc tgttttatcc cgatcacgat aatttctggc
     5701 tggggttgat tcccggtatt ccggcggtgc tggcgttttt gttgagcggt cggcgggctt
     5761 cgtttcctcg cacctggcat gtgctctatt ttctgttgct gttggcgcag gtggttttac
     5821 tttgctggca accgtggctg tggctgaacg gtgaatccgt tagcggtatc ggtctggcgc
     5881 tggtagtggc ggatattgtg gcgttaatct ggctgctgac caatcgacgt ttacgcgctt
     5941 gttttaatga agaaaaagaa taacgcagag gtgcgctttg tttatgccgg atgcggcgtg
     6001 aacgccttat ccggcctaca aaacatcgaa aattcaatat attgcaggag aaagtaggcc
     6061 tgataagcgt agcgcatcag gcaattttgt gtttgtcatc aacctttaac ggcacttttt
     6121 ggtgaatttg cactccaagc aacgttattg aataaccaaa ggaagtgaca tgaaatcgct
     6181 gcgtttaatg ttatgcgcta tgccgttgat gctgaccggc tgttccacga tgtcgtcagt
     6241 taactggtcg gctgctaacc cgtggaactg gtttggatcg tccaccaaag tgagcgagca
     6301 gggcgtgggt gaattaacgg cgtccacacc actgcaagaa caagccattg ctgatgcgct
     6361 tgatggcgat tatcgcctgc gcagcggaat gaaaaccgcg aacggcaacg tggtgcgctt
     6421 ttttgaagtg atgaaaggcg acaacgtggc gatggtgatt aacggcgatc aaggcacgat
     6481 cagtcgcatt gacgtgctgg atagcgatat tcctgctgac accggtgtta aaatcggtac
     6541 accgtttagc gacctttaca gcaaagcatt tggcaattgc caaaaagctg atggtgatga
     6601 taatcgtgcc gtcgaatgta aagccgaagg cagtcaacat attagctacc agttcagagg
     6661 ggaatggcgt ggtcctgaag ggttaatgcc ttcggacgat accctgaaaa actggaaagt
     6721 cagtaaaatt atctggcggc gttaatttgc gtctgaacaa accgccgccg cgaaaaaaca
     6781 cgtaaaatag cgctcaacaa tgccacggat tgcgtggcat tcttattttc aggaggaaca
     6841 atgtctcagg ttcagagtgg cattttgcca gaacattgcc gcgcggcgat ttggatcgaa
     6901 gccaacgtga aaggggaagt tgacgccctg cgtgcggcca gtaaaacatt tgccgacaaa
     6961 ctggcaactt ttgaagcgaa attcccggac gcgcatcttg gtgcggtggt tgcctttggt
     7021 aacaacacct ggcgcgctct gagcggcggc gttggggcag aagagctgaa agattttccg
     7081 ggctacggta aaggccttgc gccgacgacc cagttcgatg tgttgatcca cattctttct
     7141 ctgcgtcacg acgtaaactt ctctgtcgct caggcggcga tggaagcctt tggtgactgc
     7201 attgaagtga aagaagagat ccacggcttc cgttgggtgg aagagcgtga cctgagcggc
     7261 tttgttgacg gtacagaaaa cccggcgggt gaagagacgc gccgcgaagt ggcggttatc
     7321 aaagacggcg tggatgccgg cggcagctat gtgtttgtgc agcgttggga gcacaacctg
     7381 aagcagctca accggatgag cgttcacgat caggagatga tgatcgggcg caccaaagag
     7441 gccaacgaag agattgacgg cgacgaacgt ccggaaacct ctcacctcac ccgcgttgat
     7501 ctgaaagaag atggcaaagg gctgaagatt gttcgccaga gcctgccgta cggcaccgcc
     7561 agtggcactc acggtctgta cttctgcgcc tactgcgcgc gtctgcataa cattgagcag
     7621 caactgctga gcatgtttgg cgataccgat ggtaagcgtg atgcgatgtt gcgcttcacc
     7681 aaaccggtaa ccggcggcta ttacttcgcg ccgtcgctgg acaagctgat ggcgctgtaa
     7741 gcgtttctga ttctatgatt aaaggccagc cagtaacaaa actgactggc ctttttatta
     7801 cttttgcttc aacgccgcat acacctgatc aactacccaa ccataagttg caatcggtaa
     7861 ctgaccgctt tcgaacatat tgggattttt ttgtggatcg gcaactggcg aatggggctt
     7921 gttgcttaac atcacaatcg ccatatgatt gaccggatcg ataacggtta ctgtcccggt
     7981 ccagccagtg tgaccgtagg tttgcgggct tgccagcgtg ccaaacgtcg gcgtcatggt
     8041 ggcattacca ttcacgcgcc agccgaggcc aaaagtggca tcttccttag agctggtggt
     8101 gaacatcttc accgtttccg cactgaacag ctgcacatcg ccatagcccc cgccgttcag
     8161 catcgtttgc attaacaccg caatatcgcc ggtattggaa aacaaacctg cgtgcccgga
     8221 aacgccgccc atcgaataga aggctttttc atcgtgcacc tgaccccaga gagtggaggt
     8281 gcggatattc gggaaatgga tcacgccatc gcgggtattg ccgttcaatt ccgtggcggc
     8341 aatttgttgc ggtttaaagc ctttcagcag cgggttaaat accgtatgtg tcaggccgag
     8401 cgggcgataa atcgactctt caacatagcg gtcaagcggc tgaccggtaa ccgactcgac
     8461 gataaatcca agcagcatat aatcgacatc gctgtagata tgtttgctgc cgggctgata
     8521 ttccagcggc gtgcgcttga tcatctccag cgtctggcct ttatcctagg aatataacgc
     8581 gcccgcgacc gctttattcg ggtattgcgg atctgccggg aaaccgccgc tgtgatgcag
     8641 caggtcagaa atccgcaggg tgtttttgcc tttaatggtg tcatttggac tatcggcaaa
     8701 ccccggaata tatttcgcaa tccgatcgtc aggatgcagt ttgccttcgc tcatcagctt
     8761 ttgcagggcg aagttcgtgg cgtacatttt ggtgtttgag gccaggtcat acagcgtccc
     8821 ggtggtggct ttgacaggct gttccatcaa cacgctgcca tcgtactttt tcgccgctcc
     8881 ccaggctttg cgatacacaa tctgattatc tttaatgatc agcaggttta cgccgggata
     8941 accggcatca acttgctggc taatccagcg atccatctgg ttaagccgtt tgacgttaaa
     9001 ccctgctttc tctggcgagc tttctgtcag aacagggtat tttgcggcgc tgacgctaca
     9061 actaacagcc agcagagaaa gatagagcat tgtccgtttc atgatatcag tccagattga
     9121 cgttacggca gccaaagagc gtggtgaaaa taaacccgca aacccatgcc accagaatcc
     9181 cgccagcata aaccgccatg gccggaagga tgccttgtgc ggaagtcatc agcggcagcg
     9241 ctaccagacc agacggccca aaggcgctgt ttaagcccat cggcagaccc caccaggcta
     9301 tcaggccgat aaacaaaccg cccgccgcac cgcctaaaca ggcggtgaca aacggcttca
     9361 tgcggggcag ggtgacaccg taaatcagcg gttcaccaac gcccagcagg ccgggaataa
     9421 tcgccccgcg tacctgactg cgtagcgcac tgtgcggttg cgaccgccag tagagtgcca
     9481 gcgccgcgcc cacctggccc gcgcctgcca ttgaaaggat gggaaataag ctgttgaatc
     9541 cctggctgtc cattaacgcg aggtaaacag gaataaagcc ctgatgcacg ccaaatacca
     9601 cggcgatcag gaacagcccg gctaaaaccg cacaaccgaa cggattactg ttcaggtgca
     9661 taaacagcca tgacatacct tcgaataacc agccacccag cggcataatg atcaggtacg
     9721 caagcgtagc ggtgatcagc agggtgatta acgaggtcag cagcatgtcg agatcgtccg
     9781 gcataaagcg gcgcaccatg ccttcaatgc gggcgcaggc ccacgcggca atcagcacgc
     9841 cgataatatt gccgcgcgga tcgatgggca gaccaaagaa atcgtgaaaa ccggcgtagt
     9901 aaccggtggt tgcagcgggg ttataaccga gcaaaaagag tgcggcaata attgcgccat
     9961 tgacgcccgt gccgccaaat gcctgggcgg cgttatagcc caccagaatc accaggaagg
    10021 tgaacaaacc tttgctgaac accttcataa aattcagcgc gtcgggtagc gtgccctgag
    10081 catctgccgg aacgtgcatc accgtggcaa ttaacgtcgc tatccccagc agcagaccgg
    10141 cggcaataaa accggggatc agcggcgtga agatggtggc gaatttggcg agaaattgtt
    10201 gcacgccgga ggtttgtttg gcttttaact gacgtttgtt ctgggcggcg atttctgcgg
    10261 catcctgtac cggtgcctcg cccagcagct cgctcatcgc ttttgcggca cgatgcgctt
    10321 taccaggtcc aaaaacgacc tgcacctggt cgctggtcaa aatgacgctc ttcacgcctt
    10381 ccagcgtttt gatattggga tcaaccagtg aactgtcatg tacacccaga cgcaggcgcg
    10441 tcatacagtt accacaactg gcgatatttc ccggtccgcc gacacgggta agaatggtgt
    10501 tcagaagttc actgctgatc tctttggcca tgggttattc cttgtctaaa acctgacgaa
    10561 taaagccgcc gtgttgatcc aggcgttttt tagcttctgc ggcatcgaga tttttcagca
    10621 ccatcacaat ggccgtttta cagttgcgct cgcaggcaat taacgccgct tccgcttgtt
    10681 cggcattaca tccggtggcg tttttaacga tattgacctg gcgcacatgc agtttttcgt
    10741 tggtggcgac cacatcgacc ataaggttgc cgaacacttt gccggattta atcatcagcc
    10801 cggtggaaag catattgagc accagtttct gcgctgtacc tgctttcatc cgcgaagaac
    10861 cggtaacaac ttcggcacct acaatcggtg taatggcaaa ctcagcggtg gttgaaacgg
    10921 cgctccccgg attacaggaa attcccactg tgcggcagcc gagctggcgt gcgtattcca
    10981 gtccggcaat cacatacggc gtgcgaccgc tggcagcaat gccaaccacc acatcctgtg
    11041 ccgttaaatt aatatttttc agatcattaa caccgccttc ccggctatct tccgcgcctt
    11101 ccaccgcgtg ctgaatggca tattcgccgc cagcaatcaa accaaccacc agacccggtt
    11161 tcacgccgta ggtgggcgga cattcgctgg catccagaat ccccagacga ccggatgttc
    11221 ccgcaccgag gtaaatcaga cgcccgccgc cgcttacctg ggcgtggata acatcgatcg
    11281 ccgcggcgat atccggcagt acgcgctcaa cggcaagcgg tacggtttta tcttcatcgt
    11341 tgataatccg gcacatttcc agcgttgata cgcggtcaat ttcagccgag gcggcgttcg
    11401 agccttcagt aatcatcttt tcaagttgca tcggtggtga ccttactatt tacgatcaaa
    11461 taattgcaga tgtatggaat catatatcct gaattgacag acttaaagga atcattgagt
    11521 ctacactcgc agagtgtgaa gagcgtcata agaacttaag cggaatttat gctgccagcc
    11581 caggagagtg gaagatgttg tacctgacga aaataagcaa tgcggggagt gaattcaccg
    11641 aaaacgagca aaaaatcgct gactttctgc aggccaacgt gagtgagctg caatccgttt
    11701 cttcacggca gatggcgaaa cagctcggca ttagccagtc gagcatagtg aaatttgccc
    11761 aaaaactcgg cgcgcaagga tttactgaat tgcgtatggc gttaataggc gaatacagtg
    11821 ccagccggga gaaaacgaat accacggcgc tgcacctgca cagttcgatt accagtgatg
    11881 attcgctgga agtgattgcc cgcaaattga atcgcgaaaa agagctggcg ctggagcaaa
    11941 cctgtgcgtt gttcgactac gcgcggctgc aaaaaatcat tgaggtcatc agtaaagcac
    12001 cgtttatcca gataacgggc ctgggcggat cggcgctggt ggggcgcgat ctgtcgttca
    12061 aactgatgaa aattggctat cgcgtggcct gcgaagccga cacgcacgta caagccaccg
    12121 tttcccaggc gttgaaaaaa ggcgatgtac aaatcgctat ctcttacagc ggtagtaaaa
    12181 aagagattgt cttgtgcgta gaagcggcac gaaagcaggg ggcaacggtg attgccatta
    12241 cttctctggc tgactctccg ctgcggcggc tggcgcattt cacgctcgat accgtttccg
    12301 gtgaaaccga atggcgtagc tcgtcgatgt ctacccgcac ggcgcaaaat tctgttaccg
    12361 atttactgtt tgttggtctt gtgcaactca atgatgtggc gtcattgaaa atgattcagc
    12421 gcagcagtga gctaacacaa cgcctgaaat gatgcataaa gcagcgactg gattgagatt
    12481 ttcctgaatt agtgagctga tccgcagcaa tattttgttt atcctgtatt ttcagaggga
    12541 atggagtgta acgctctgta ttaacaagga gagcattaaa atgggtaaac tcacgggcaa
    12601 gacagcactg attacgggcg cattgcaggg aattggcgaa ggaattgcca gaacttttgc
    12661 acgtcatggc gcgaacctaa tcttgctgga tatctcccct gagatcgaaa agctggcgga
    12721 cgaactgtgt ggtcgtggtc atcgctgtac ggcggttgtc gccgatgtgc gtgacccggc
    12781 gtcggtagcc gcagctatca aacgcgcgaa ggaaaaagaa gggcgcattg atatcctggt
    12841 gaataacgca ggcgtttgtc gtctgggcag tttcctcgat atgagcgatg acgatcgcga
    12901 tttccatatt gatatcaata ttaaaggcgt atggaacgtc acgaaggcgg tgctgccgga
    12961 gatgattgcg cgcaaagatg gtcgcattgt gatgatgtct tcagtcactg gtgatatggt
    13021 ggccgatcct ggcgaaacgg cgtacgcctt aacgaaagcg gcgattgttg gcctgacaaa
    13081 atcgctggcg gtggagtacg cgcagtctgg tattcgcgtt aacgccattt gcccgggata
    13141 cgtgcgcaca ccaatggcgg aaagcattgc ccgccagtcg aacccggaag atccagagtc
    13201 ggtgctgact gaaatggcga aagcaatccc gatgcgtcgc ctcgccgatc cgctggaagt
    13261 cggcgaactg gcggccttcc tcgcatcgga tgaatccagc tatttaaccg gtacacagaa
    13321 tgtgattgat ggcggcagca cactgccgga gacggttagc gtcggtatct gattcacctc
    13381 tgtttcctcc ctgcatgtgt ggggaggatt tcgtcttgaa ctaagttcac caggctattt
    13441 tatttgtcat tttggccccg ggcagtgctc gaaatactca cgtactatgt gtacgctccg
    13501 gtttctccgc gctgtccgtg tccaaactgc ctgcaacaat tacgcctgtt gaaccaagtt
    13561 cttattccct ttttaacttc caaatcacca aacggtatat aaaaccgtta ctcctttcac
    13621 gtccgttata aatatgatgg ctattagaaa gtcattaaat ttataagggt gcgcaatggc
    13681 cgttaactta ctgaaaaaga actcactcgc gctggtcgct tctctgctgc tggcgggcca
    13741 tgtacaggca acggaactgc tgaacagttc ttatgacgtc tcccgcgagc tgtttgccgc
    13801 cctgaatccg ccgtttgaac agcaatgggc aaaagataac ggcggcgaca aactgacgat
    13861 aaaacaatct catgccgggt catcaaaaca ggcgctggca attttgcagg gcttaaaaac
    13921 cgacgttgtc acttataacc aggtgaccga cgtacaaatt ctgcacgaca aaggcaagct
    13981 gatcccggct gactggcagt cgcgcctgcc gaataatagc tcgccgttct actccaccat
    14041 gggcttcctg gtgcgtaagg gcaacccgaa gaatatccac gactggaacg acctggtgcg
    14101 ctccgacgtg aagctgattt tcccgaaccc gaaaacgtcg ggtaacgcgc gttataccta
    14161 tctggcggca tggggcgcag cggataaagc tgacggtggc gacaaagcca aaaccgaaca
    14221 gtttatgacc cagttcctga aaaacgttga agtgttcgat actggcggtc gtggcgcgac
    14281 caccactttt gccgagcgcg gcctgggcga tgtactgatc agcttcgagt cggaagtgaa
    14341 caacatccgt aaacagtatg aagcgcaagg ctttgaagtg gtgattccga aaaccaacat
    14401 tctggcggaa ttcccggtgg cgtgggtcga taaaaacgtg caggccaacg gtacggaaaa
    14461 agcagcaaaa gcctacctga actggctcta cagcccgcag gcgcagacca tcatcaccga
    14521 ctattactac cgcgtaaata acccggaagt catggacaaa ctgaaagata aattcccgca
    14581 gaccgagctg ttccgcgtgg aagacaaatt tggctcctgg ccggaagtga tgaaaaccca
    14641 cttcaccagc ggcggcgagt tagacaagct gttagcggcg gggcgtaact gatgtttgca
    14701 gtttcatcaa gacgcgtgct gccgggcttt accttaagcc tcggcaccag tctgctgttt
    14761 gtgtgcctga ttttgctgct gccgctctcc gcgctggtga tgcagctggc tcagatgagc
    14821 tgggcgcagt actgggaggt gatcaccaac ccacaagtgg tcgcggccta caaagtgacg
    14881 ctgctgtcgg cgtttgtggc atcgattttt aacggcgttt tcggtctgct gatggcgtgg
    14941 attttaacac gttatcgctt tccgggccgc acgctgcttg atgcgctgat ggatttaccc
    15001 tttgcgctgc caacggcagt cgccggttta acgctggcct cgctcttttc cgtaaacggt
    15061 ttttacggtg aatggctggc gaagtttgat atcaaagtca cctatacctg gcttggtatt
    15121 gcggtggcga tggcctttac cagcattccg tttgtggtgc gtaccgtgca gccggtgctg
    15181 gaagagttag gcccagaata tgaagaagcg gcggaaacgc ttggtgcaac gcgctggcag
    15241 agtttctgca aagtggtgct gccggaactt tctccagcgc tggtggcggg cgtggcgttg
    15301 tcgtttaccc gtagtcttgg tgagtttggc gcggtgatat ttatcgccgg gaacatcgct
    15361 tggaagacgg aagtgacgtc gctgatgatt tttgtgcgct tgcaggagtt tgattacccg
    15421 gcggcgagcg cgattgcttc ggtgatcctc gcggcatccc tgctgctgct gttctcaatt
    15481 aacactctgc aaagtcgctt tggtcggcgt gtggtaggtc attaatggcg gaagttaccc
    15541 aattgaagcg ttatgacgcg cgcccgatta actggggcaa atggtttctg attggcatcg
    15601 ggatgctggt atcggcgttc atcctgctgg tgccgatgat ttatatcttc gtacaggcgt
    15661 tcagcaaagg gctgatgccg gttttacaga atctggccga tccggacatg ctgcacgcca
    15721 tttggctgac ggtgatgatt gcgctgattg ccgtaccggt gaacctggtg ttcggcattc
    15781 tgctggcgtg gctggtaacg cgctttaact tccccggacg ccagttactg ctgacgctac
    15841 tggacattcc gtttgccgta tcgccggtgg ttgcaggtct ggtgtatctg ctgttctacg
    15901 gctctaacgg tccgctgggc ggctggctgg atgaacataa tttgcagatt atgttctcct
    15961 ggccgggaat ggtgctggtc accatctttg tgacctgtcc ttttgtggtg cgcgagctgg
    16021 tgccggtgat gttaagccag ggcagccagg aagacgaagc ggcgattttg cttggcgcgt
    16081 ctggctggca gatgttccgt cgcgtcacat tgccgaacat tcgctgggcg ctgctgtatg
    16141 gtgtggtgct aaccaacgcc cgcgcgattg gcgagtttgg tgcggtgtcg gtggtttccg
    16201 gctcgattcg cggcgaaaca ctttcgctgc cgttacagat tgaattgctg gagcaggact
    16261 acaacaccgt cggctccttt accgccgccg cgctgttgac gctgatggcg attatcaccc
    16321 tgtttttaaa gagtatgttg cagtggcgtc tggagaatca ggaaaaacgc gcgcagcagg
    16381 aggagcatca tgagcattga gattgccaat attaagaagt cgtttggtcg cacccaggtg
    16441 ctgaacgata tctcactgga tattccttct ggtcagatgg tcgcgctgct ggggccgtcc
    16501 ggttccggaa aaaccacgct gctgcgcatt attgccgggc tggagcatca aaccagcggg
    16561 catattcgct tccacggtac cgacgtgagc cgcctgcacg cacgcgatcg taaagtcggt
    16621 ttcgtgttcc agcattacgc gctgttccgt catatgacgg tgttcgacaa tatcgctttt
    16681 ggcctgacgg tgctgccacg tcgcgagcgc ccgaatgccg cagccatcaa agcgaaagtg
    16741 acaaaattgc tggagatggt ccagcttgcg catctggcgg atcgttatcc ggcacagctt
    16801 tccggcggcc agaaacagcg cgtggcgctg gcgcgcgcgc tggctgttga accgcaaatt
    16861 ctcctgcttg atgaaccgtt tggcgcgctg gatgcgcagg tgcgtaaaga gctgcgtcgc
    16921 tggctgcgtc aactgcatga agagctgaaa ttcaccagcg tgttcgtgac ccacgaccag
    16981 gaagaagcga ccgaagtagc tgatcgtgta gtggtgatga gccagggcaa tatcgagcag
    17041 gctgatgcgc cggaccaggt atggcgcgaa ccagcgaccc gatttgtgct cgaatttatg
    17101 ggcgaagtga accgcctgca gggaaccatt cgcggcgggc agttccatgt tggcgcacat
    17161 cgctggccgc tggggtatac gcctgcgtat caggggccgg tggatctctt cctgcgcccg
    17221 tgggaagtgg atatcagccg ccgtaccagt ctcgattcgc cgctgccggt acaggtactg
    17281 gaagccagcc cgaaaggtca ctacacccaa ttagtggtgc agccgctggg gtggtacaac
    17341 gaaccgctga cggtcgtgat gcatggcgac gatgccccgc agcgtggcga gcgtttattc
    17401 gttggtctgc aacatgcgcg gctgtataac ggcgacgagc gtatcgaaac ccgagatgag
    17461 gaacttgctc tcgcacaaag cgcctgatag gttgagtgaa tgttaaacgc ccggaggcgc
    17521 ttcccgcgat ccgggctttt taatggcaag gtttgtaacc tgtagacctg ataagacgcg
    17581 caagcgtcgc atcaggcaac accacgtatg gatagagatc gtgagtacat tagaacaaac
    17641 aataggcaat acgcctctgg taaagttgca gcgaatgggg ccgaataacg gcagtgaagt
    17701 ctggttgaaa ctggaaggca ataacccggc aggttcggtg aaagatcgtg cggcactttc
    17761 gatgatcgtc gaggcggaaa agcgcgggga aattaaaccg ggtgatgtgt taatcgaagc
    17821 caccagtggt aacaccggca ttgcgctggc aatgattgcc gcgcttaaag gctatcgcat
    17881 gaaattgctg atgcccgaca acatgagcca ggaacgccgt gcggcgatgc gtgcttatgg
    17941 tgcggaactg attcttgtca ccaaagagca gggcatggaa ggtgcgcgcg atctggcgct
    18001 ggagatggcg aatcgtggcg aaggaaagct gctcgatcag ttcaataatc ccgataaccc
    18061 ttacgcccat tacaccacta ccgggccgga aatctggcag caaaccggcg ggcgcattac
    18121 ccattttgtc tccagcatgg gaacaaccgg cactatcacc ggcgtttcac gctttatgcg
    18181 cgaacaatcc aaaccggtga ccattgtcgg cctgcagccg gaagaaggca gcagtattcc
    18241 cggcattcgc cgctggcctg cggaatatct gccggggatt ttcaacgctt ctctggtgga
    18301 tgaggtgctg gatattcatc agcgcgatgc ggaaaacacc atgcgcgaac tggcggtgcg
    18361 ggaaggaata ttctgtggcg tcagctcagg cggcgcggtt gctggagcac tgcgggtggc
    18421 aaaagctaac cctggtgcgg tggtggtagc gatcatctgc gatcgtggcg atcgctacct
    18481 ctccaccgga gtgtttgggg aagagcattt tagccagggg gcggggattt aagattattg
    18541 cgcaggcacc gttttatccg tctgcgcaat tagcgcatgt aaaaaaggct gtgcgttctc
    18601 atcgctctta ccggggatct tcacaatata cggcttcccg gtaatcgatg acgacgaagc
    18661 aaccttatca ataaactgct cggcagtgtc aatgcggtta cgggtattgc cgagcttcag
    18721 acgcagatga gaaaccgctt catcgcaggt atgttcatca ccgttgcgca caaatatcaa
    18781 atcctttttc tgtgataatc cctccagcat ggcgttgatg cgggcttctt catgggcagt
    18841 taacttcgcg taagcgggga gtgtcattag tagtgtaatg accagacaaa tgattttctt
    18901 cattgaatta ccttatcctt gttttgtaat agccgatttg actgccagaa tcaggtaaag
    18961 tttcggttgg aatacgatga ggatcatcgg caaggagaaa aaattaaatg agtagtttgt
    19021 tgttgtttaa cgataagagc agggcgctgc aggcggatat cgtcgccgtg cagtcgcagg
    19081 tggtttacgg cagcgtgggc aacagcattg ccgtgcctgc tatcaaacag aacggtctga
    19141 atgtttttgc cgtgccgacg gtattgctga gcaatacgcc gcattatgac actttctacg
    19201 gtggtgcgat tccggatgaa tggtttagcg gttatttgcg tgcgcttcag gagcgtgatg
    19261 cgctgcgcca gctgcgcgct gtaaccacgg gctatatggg aacggcatcg caaatcaaaa
    19321 tccttgccga gtggctgact gcgctacgca aagaccatcc tgacctattg atcatggtcg
    19381 atccggtgat tggcgatatt gatagcggaa tttatgtcaa acctgacctt cccgaggcgt
    19441 atcgacaata tttactgccg ctggcgcagg gaattacccc caatatcttt gagctggaaa
    19501 tcctgaccgg taaaaattgc cgcgatctcg acagtgccat tgctgccgca aaaagtctgc
    19561 tttcagacac attaaaatgg gtggtgatta ccagcgcctc cggtaatgaa gaaaatcagg
    19621 agatgcaggt ggtggtggtc agtgccgaca gcgtgaatgt catttcccat tcacgggtaa
    19681 aaaccgacct gaaagggact ggcgacctgt tttgtgctca gcttatcagt ggtttgctga
    19741 aagggaaggc gttaaacgat gcggtgcacc gtgcggggtt gcgcgtactg gaagtgatgc
    19801 gctacaccca gcagcatgag agcgatgaat tgattttgcc gccgctggcg gaagcataaa
    19861 aaatg
//
LOCUS       DAJTSL010000068        19589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTSL010000068 DAJTSL010000000
VERSION     DAJTSL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.068"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..173)
                     /locus_tag="OO433_002167"
     CDS             complement(<1..173)
                     /locus_tag="OO433_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050639.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNV"
     gene            complement(209..1063)
                     /locus_tag="OO433_002168"
     CDS             complement(209..1063)
                     /locus_tag="OO433_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HCR6050640.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            1406..2236
                     /locus_tag="OO433_002169"
     CDS             1406..2236
                     /locus_tag="OO433_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6050641.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEINHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            2268..3203
                     /locus_tag="OO433_002170"
     CDS             2268..3203
                     /locus_tag="OO433_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6050642.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            3296..3538
                     /locus_tag="OO433_002171"
     CDS             3296..3538
                     /locus_tag="OO433_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6050643.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPDVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            3528..4979
                     /gene="panF"
                     /locus_tag="OO433_002172"
     CDS             3528..4979
                     /gene="panF"
                     /locus_tag="OO433_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6050644.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFY
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            4991..5872
                     /gene="prmA"
                     /locus_tag="OO433_002173"
     CDS             4991..5872
                     /gene="prmA"
                     /locus_tag="OO433_002173"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6050645.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            6201..7166
                     /gene="dusB"
                     /locus_tag="OO433_002174"
     CDS             6201..7166
                     /gene="dusB"
                     /locus_tag="OO433_002174"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6050646.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            7192..7488
                     /gene="fis"
                     /locus_tag="OO433_002175"
     CDS             7192..7488
                     /gene="fis"
                     /locus_tag="OO433_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6050647.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            7574..8458
                     /gene="yhdJ"
                     /locus_tag="OO433_002176"
     CDS             7574..8458
                     /gene="yhdJ"
                     /locus_tag="OO433_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6050648.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYDPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            8542..8721
                     /locus_tag="OO433_002177"
     CDS             8542..8721
                     /locus_tag="OO433_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6050649.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(8724..9386)
                     /gene="acrS"
                     /locus_tag="OO433_002178"
     CDS             complement(8724..9386)
                     /gene="acrS"
                     /locus_tag="OO433_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCR6050650.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            9785..10942
                     /gene="acrE"
                     /locus_tag="OO433_002179"
     CDS             9785..10942
                     /gene="acrE"
                     /locus_tag="OO433_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCR6050651.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYNSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     GTVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMYVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10954..14058
                     /gene="acrF"
                     /locus_tag="OO433_002180"
     CDS             10954..14058
                     /gene="acrF"
                     /locus_tag="OO433_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HCR6050652.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTNDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALSV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFLPVFFVVIRR
                     CFKG"
     gene            14311..14532
                     /locus_tag="OO433_002181"
     CDS             14311..14532
                     /locus_tag="OO433_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050653.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGCLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIAMSK"
     gene            14963..16006
                     /locus_tag="OO433_002182"
     CDS             14963..16006
                     /locus_tag="OO433_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6050654.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LLGFSYADADGKFSGIDVDVDVDVDVCRSVAAAVFGDDTKVKYTPLTAKERFTALQSG
                     EVDLLSRNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAG
                     TDTELNVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNP
                     AEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPA
                     TPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNL
                     WNNGGIQYAPPVR"
     gene            16074..17244
                     /locus_tag="OO433_002183"
                     /pseudo
     CDS             16074..17244
                     /locus_tag="OO433_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            17254..18357
                     /locus_tag="OO433_002184"
     CDS             17254..18357
                     /locus_tag="OO433_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6050655.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     VLLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            18365..19122
                     /locus_tag="OO433_002185"
                     /pseudo
     CDS             18365..19122
                     /locus_tag="OO433_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(19354..19469)
                     /gene="rrf"
                     /locus_tag="OO433_002186"
     rRNA            complement(19354..19469)
                     /gene="rrf"
                     /locus_tag="OO433_002186"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(19509..19584)
                     /locus_tag="OO433_002187"
     tRNA            complement(19509..19584)
                     /locus_tag="OO433_002187"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19549..19551),aa:Thr,seq:ggt)
BASE COUNT         4806 a         4680 c         4900 g         5203 t
ORIGIN      
        1 ttgacattgt ctttttttgc ctcttctttc atggcattcg caagatcaat atagaaagga
       61 tgttgctgtg ttaataatga cgcaccaatt ttatactctt ttgccaacga attttgcgag
      121 aacaatgcca ggctcaccaa catagctgaa ccaagtaatt taatattttt cataaacaat
      181 cctcattgtt tttgtagggt acaacgtctt aaatttttcc ttcactacca cagacaccaa
      241 taatacgagt tacgacttct ttcatggcgg cctttgcggg ctgcatatag tggcgtggat
      301 catttgccgc aggattgttg tagaaatatc cttttaacgc atcggcaaac gcaattttca
      361 attctgtcgc gacattcact ttacaaatac cgcggcgaat acagtgctga acgtcctgtt
      421 ctgataagcc cgaagcacca tgcaacacca gtggaatctc cactcgctga cgaatctgtt
      481 ccagccgcac aaaatccagt cgtggttcat gcgcatataa accatgtgct gtgccgattg
      541 ctacggccag ggagtcaatt tgggtacgtt gaacaaactc tgcagctgcg gctggtgagg
      601 tataagcgga atctttttca tccaccagca gatcgtcctc ctggccgcca agacgcccta
      661 gctcggcctc aacgctaaca tcaaagcgat ggcaaaaggt ggtaacttct tgcactcgcg
      721 caatgttgtc ttcaaatggc aaatgggagc catcaatcat gacagaacgg atccctgcgc
      781 gaaccttgtt aaaaatgtca tcactctctt catgatggtc gagatgcagg actaacggca
      841 ggttgtactc gcgagccagc gattgtacta acgaaattaa acttcccacg ccaccataac
      901 gataagtacc gggtgttccc gccagcataa cgggagagcg aagttgtgca gcgctctcaa
      961 caactacctg taaagtttca aggttatgaa tgttgaaagc ggggacggca tatccctctg
     1021 actgggcttt tttcagcatg tttttgcttg aaatgatgta catcgcagct cctgttattt
     1081 gtcgttcgcc ttacaaaatg aagaaagaga atattcactt tcattttgaa agcaaactag
     1141 cacagcaaaa gcggcaaaaa gcgtgataca tgtcgaatat catcaaaata acttacaaaa
     1201 aaataactta atttgttttt cactttacaa gtgaaaccaa taacatctct gcataaatat
     1261 gaataaattc atttattttt caatgaatta atctattttt gttgcgattt ttgacccatg
     1321 attttctttt cgaattgtcg atattattga aggataactt tcaaagaaaa atacaagggg
     1381 ctatagtgtt acgcattaaa ggccaatgaa gagtgttaac gtgccgagaa aatcgtctaa
     1441 cctactgaaa cgcagattaa aaattgcaga aattgttgcc gcccaaggtg aaatcaaagt
     1501 tgatgactta tctgcgcaac ttggtgtctc tggcgtgacg atccgcggcg atctcagtta
     1561 tctggagcaa cagggatatt taaagcgatc atttggcggt gctattgcga caccactctc
     1621 ctctgaacca gaagcgatac aaccgatcgc gccattgtct ctggcacaac aaatggaaat
     1681 agcacgacat tgcgcacggt taattcgcga tcgtgacacg ctatttcttg gacatgggac
     1741 aatctgccgt aagatcattc cattgctcag cgaagtgaaa aaactgcgtc tgatcaccaa
     1801 cgatcccgaa catgcgctgc tggcaaatca gtttattgat ggcgaaataa tactggctgg
     1861 cagtgaaatg ctgcgccccg acaccgcact ggccggaaaa ccgcttgaac aggcacttca
     1921 gcatttcacc attacacata gcattctgga aatcaaccat ctggatgctg atggtacttt
     1981 aaatatcaac gttcccagac tggccgctgc ctggcagctc tgtttcgatc atgcccagaa
     2041 taagacggtt atcgttgccg ccgatcctgc gccatcaacg gctgctatcg ggcatttgca
     2101 tcaggcagaa aatgtgatcg tcagccgcag cattaacgaa caatatcagc aacaattgca
     2161 gtcggctgat ttcgttatcc tttataccag taatgagtgc tttacctggt ccaatcgcga
     2221 aaggcttcag gaatgagagg ggttttgtga caataactat ctgcgctatg ggcgaattgc
     2281 tggccgagtt tttgtcccgc aacccacatc aaaaattcac tcagcctggg gagtttatcg
     2341 ggccatttcc cagcggtgcg ccagcaattt ttgctgctca ggtggcaaaa ctgtcccatc
     2401 gggccatctt cttcggatgt gttggtaatg atgattttgg ccgactcatt atagagcgcc
     2461 tccgtcatga aggtgtcatt accgatggga tccatgttat gaacaatgcc gtcacaggta
     2521 cggcgttcgt gagttatcaa aatccccagc agcgcgattt cgtctttaat atccctaaca
     2581 gcgcctgcgg cttgtttact gctgagcaca ttgataagga tctgctaaaa cagtgtaacc
     2641 atctccatat tgtgggctca tcattgttct catttcgcat gatcgatgtc atgcgtaaag
     2701 caataacgac gatcaaatcg gctggcggca ccgtttcttt cgatcccaat attcgcaaag
     2761 agatgctgag cattcctgaa atggcgcagg ctctcgatta tttgattgaa tatacggata
     2821 ttttcatccc cagcgaaagc gaactccctt tcttcgcgcg tcacaaaaat ctgtcagaag
     2881 aacagattgt tagcgatctt ctccacggcg gcgtaaaaca tgtggcgata aaacgcgccc
     2941 agcgtggggc cagctattac aagcttaaaa acggtacatt acacgcccag catgttacag
     3001 gccacgatat cgaaattatc gatccaacgg gtgcaggcga ctgctttggc gcaacgttta
     3061 tcactctttt cttatccggt ttcccggcac ataaggcact gcaatatgca aatgccagcg
     3121 gcgcgctcgc cgtaatgcgg caaggtccga tggaagggat atcctcactg gctgacattg
     3181 aagacttttt gcagcagcac tgaacggcaa ttttctgtca gattattttc cctgcctgaa
     3241 gagttgccgt agaattgccc gtttctcgta tccccccatg aggaggaacc cttccgtgga
     3301 cactcgtttt gttcaggccc ataaagaggc gcgctgggcg ctggggctga cccttttgta
     3361 tctggcagtt tggttagcag ccgcttactt acctgacgtt gcccccggtt ttaccggctt
     3421 tccgcgctgg tttgagatgg cctgcatcct gacgccgctg ctgtttattg gactgtgctg
     3481 ggcgatggtg aaatttatct atcgcgatat cccactggag gatgacgatg cagcttgaag
     3541 tgattctacc gctggtcgcc tatctggtgg tggtgttcgg tatctctgtt tatgcgatgc
     3601 gtaaacggag caccggtacc ttccttaatg agtatttcct cggcagccgc tctatgggcg
     3661 gtattgtgct ggcgatgacg ctcaccgcga cctatatcag tgcaagttcg tttatcggcg
     3721 ggcctggagc tgcttataaa tacggcctgg gttgggtatt gctggcgatg attcagcttc
     3781 ctgcagtctg gctttcgctc ggtattctcg gcaagaagtt cgcgattctt gcgcgccgct
     3841 acaatgcagt gacgctgaac gacatgctgt ttgcccgcta ccagagtcgt cttctggtgt
     3901 ggctggcgag tttgagtttg ctggttgcgt tcgttggggc aatgaccgtg cagtttatcg
     3961 gcggtgcgcg cctgctggaa accgcggcgg gtattcctta tgaaaccggg ctgctgattt
     4021 tcggtatcag cattgcgttg tataccgcct ttggcggctt tcgcgccagc gtgctgaacg
     4081 acaccatgca agggcttgtg atgctgattg gcaccgttgt gctgcttatt ggcgtggtac
     4141 atgccgcagg tggtttaagt aatgcggtag agacgctgca aaccatcgat ccgcaactgg
     4201 ttacgccaca aggcgctgac gatattctgt cgcctgcctt tatgacgtcg ttctgggtac
     4261 tggtgtgctt tggcgtgatt ggcctgccgc atactgcggt gcgctgtatc tcttataaag
     4321 acagcaaagc ggtacaccgg gggatcatca tcggtacgat tgtagtcgca atcctgatgt
     4381 tcggtatgca cctggccgga gcgttaggtc gtgcagtgat ccccgatctc accgtaccgg
     4441 atctggtgat cccgacgtta atggtaaaag tgctgccacc gtttgctgcc gggatctttc
     4501 tggctgcacc tatggctgcg atcatgtcga caattaacgc ccaattgcta caaagttccg
     4561 ctacgatcat taaagatctc tatctgaata tccgtccgga tcaaatgcaa aacgagacgc
     4621 gtctgaagcg gatgtcggcg gtaattacgt tagttctcgg cgcgttgctg ctgcttgccg
     4681 cctggaagcc gccagaaatg atcatctggc tgaatttgct ggccttcggt gggttggaag
     4741 ccgttttcct gtggccgctg gtgctgggtc tttactggga acgcgccaac gccaaaggcg
     4801 cgctaagtgc gatgatcgtt ggcggcgtgc tgtatgccgt actcgcgacg ctgaatattc
     4861 agtacctggg cttctaccct atcgtgccct cgttactact aagtttgctg gctttcctgg
     4921 tcggaaaccg tttcggtaca tccgtcccgc aagctaccgt tttgactact gataaataaa
     4981 gagttttgcc atgccttgga tccaactgaa actgaacacc accggcgcga acgctgaaga
     5041 tcttagcgat gcgctgatgg aagcgggtgc cgtttctatc acttttcagg atacccacga
     5101 tacgccagta tttgagccgc tgccgggcga aacgcgcctg tggggcgaca ccgatgtgat
     5161 tggtctgttc gacgctgaaa ccgatatgaa cgacgtggtg gcgattctgg aaaaccatcc
     5221 gctgctcggc gcaggcttcg cgcataaaat cgaacaactg gaagataaag actgggagcg
     5281 cgaatggatg gataatttcc acccgatgcg ctttggtgaa cgactgtgga tctgcccaag
     5341 ctggcgtgat gtgccggacg aaaacgccgt caacgtgatg ttagacccag ggctggcgtt
     5401 tggtacgggt acccatccaa ctacctctct gtgcctacaa tggctcgaca gcctcgattt
     5461 aactggtaaa acagtcatcg actttggctg cggttccggc attctggcta tcgcggcgct
     5521 gaaactgggt gcagcaaaag ccattggtat tgatatcgat ccgcaggcga ttcaggctag
     5581 ccgcgacaac gccgaacgta atggcgtttc tgaccgtctg gaactctact taccgaaaga
     5641 tcagccagaa gaaatgaaag ccgacgtggt ggtcgcgaac atccttgcag gcccattacg
     5701 tgaactggca ccgttaatca gcgtcctgcc ggtttcaggc ggtttgctgg gcctttccgg
     5761 tattctggca agccaggcag agagcgtttg tgaagcttat gccgatagct tcgcactgga
     5821 cccggtcgtg gaaaaagaag agtggtgccg tattaccggt cgtaagaatt aaccttcgca
     5881 tcgccgtagg gtgacgcggg gcgaagtgcg agcaagctca caaaaggcac gtaaatttgc
     5941 cggttattta cgcaaatttg cgtgccaaaa ttttcattca taaagaaaaa ttgagaactt
     6001 actcaaattt ctttgaatgt aaattttagt cactattttc taatatgatg atttttatga
     6061 gtaattatcg caccacgctc attttaaatg caattctttg atccatctca gaggattggt
     6121 caaagtttgg cctttcatct cgtgcaaaaa atgcgtaata tacgccgcct tgcagtcaca
     6181 gtatggtcat ttcttaactc atgcgcatcg gacaatatca gctcagaaat cgcctgatcg
     6241 cagcgcccat ggctggcatt acagacagac cttttcggac gttgtgctac gagatgggag
     6301 ccggattgac agtatccgag atgatgtctt ctaacccaca ggtttgggaa agcgacaaat
     6361 ctcgtttacg gatggtgcac attgatgaac ccggtattcg caccgtgcaa attgctggta
     6421 gcgatccgaa agaaatggca gatgcagcac gtattaacgt ggaaagcggt gcccagatta
     6481 ttgatatcaa tatgggttgc ccggctaaaa aagtgaatcg caagctcgca ggttcagccc
     6541 tcttgcagta cccggatgtc gttaaatcga tccttaccga ggtcgtcaat gcagtggacg
     6601 ttcctgttac cctgaagatt cgcactggct gggcaccgga acaccgtaac tgcgaagaga
     6661 ttgcccaact ggctgaagac tgtggcattc aggctctgac cattcatggc cgtacacgcg
     6721 cctgtttgtt caatggagaa gctgagtacg acagtattcg ggcagttaag cagaaagttt
     6781 ccattccggt tatcgcgaat ggcgacatta ctgacccgct taaagccaga gctgtgctcg
     6841 actatacagg ggcggatgcc ctgatgatag gccgcgcagc tcagggaaga ccctggatct
     6901 ttcgggaaat ccagcattat ctggacactg gggagttgct gcccccactg cctttggcag
     6961 aggttaagcg cttgctttgc gcgcacgttc gggaactgca tgacttttat ggtccggcaa
     7021 aagggtaccg aattgcacgt aaacacgttt cctggtatct ccaggaacac gctccaaatg
     7081 accagtttcg gcgcacattc aacgccattg aggatgccag cgaacagctg gaggcgttgg
     7141 aggcatactt cgaaaatttt gcgtaaacag aaataaagag ctgacagaac tatgttcgaa
     7201 caacgcgtaa attctgacgt actgaccgtt tctaccgtta actctcagga tcaggtaacc
     7261 caaaaacccc tgcgtgactc ggttaaacag gcactgaaga actattttgc tcaactgaat
     7321 ggtcaggatg tgaatgacct ctatgagctg gtactggctg aagtagaaca gcccctgttg
     7381 gacatggtga tgcaatacac ccgtggtaac cagacccgtg ctgcgctgat gatgggcatc
     7441 aaccgtggta cgctgcgtaa aaaattgaaa aaatacggca tgaactaatt caggttagct
     7501 aaatgcttga ttaaaaaggc gctactcggc atggggaagc gcctttttta taggtgtcac
     7561 aaagggagtg accatgagaa caggatgtga accgacccgg tttggtaatg aagctaagac
     7621 cattattcac ggtgatgcgc ttgccgaact taaaaagatc cccgccgaaa gtgtcgatct
     7681 gatctttgcc gacccaccat ataacatcgg taaaaatttt gatggtctga tcgaagcctg
     7741 gaaagaagat ctgtttatcg actggctgtt tgaagtgatt gcagagtgcc accgcgttct
     7801 gaaaaagcag ggcagcatgt acatcatgaa cagcacggaa aacatgccct ttatcgatct
     7861 ccagtgccgc aagcttttta ccatcaaaag tcgcatcgtc tggtcatatg acagttctgg
     7921 agtacaggca aaaaaacact acggctccat gtacgacccc atcctgatga tggtgaaaga
     7981 cgcaaagaac tacacattca acggtgatgc tattctggta gaagccaaaa ccggatcgca
     8041 gcgcgcgttg atcgattatc gcaaaaatcc tccacagcca tacaatcatc aaaaagtacc
     8101 gggtaacgtc tgggattttc cgcgcgtgcg ttatttgatg gatgaatatg agaaccaccc
     8161 gacgcaaaaa ccggaagcct tactgaaacg cattattctc gcctcttcca acccaggcga
     8221 tatcgttctc gacccgtttg ccggtagctt tactaccggt gccgtagccg tctccagcgg
     8281 gcgaaaattc attggtattg agatcaacag cgagtacatc aaaatggggc ttcgacggct
     8341 ggatgtcgcg tcgcattact ctgcggaaga actggcgaaa gtgaaaaaaa gaaagaccgg
     8401 caacctgtca aaacgaagcc ggttaagcga agttgacccc gatctcattg caaagtaaag
     8461 gaagtgtaag cctggttttc agattattca tttcgtgtat atttcctgcc agacttggtt
     8521 aaacatgcac aggctcaggt aatgattcgc aagtattggt ggctcgtcgt tttcgctgtc
     8581 ttcgtttttc tgtttgatac tttactgatg cagtggattg aactgctgac aacagaaaca
     8641 gacaaatgcc gtaatatgaa ctcagttaat ccactaaaac tggttaactg tgacgaactg
     8701 aattttcagg acagaatgtg aatttacatg acacttaatt cattcgtttg atgaattaat
     8761 ttcgttatgt tttcatctgg catgaacatt cttaatacgt tatcgaccag cgcgggtgct
     8821 tgtttataaa gatcataacc cgccatattc attaaccagt tttgaacaat tccgctgaag
     8881 gcaccattaa taataatcat cacaacatct aaatcgaggt tatttgctac acaaccttgt
     8941 tgctgacacg cctgcaatac ttcgcggaga gtctgcggat taaagcccat cttttcgcgt
     9001 atcactccct cggccagcat ctcatcatta aattcacatt tgtgatataa gattttcagc
     9061 aacgcctgct ggcggggaat tttggcaata tattgcaagc cgacaatcaa tttttcacgc
     9121 aattgttgaa acgggtcatg ctctaatcca gccgtcaagt gttcctggat taactcccgc
     9181 aatgaaggct gttgcaacca catctcatta aacagttgag tcttgttttc gaagtgccag
     9241 tagatagcgc cacgcgtaac gttagcggcg tcggcaatgt cgttgagcgt cgtcttgctt
     9301 acgccatgct gcgcaaactg ggcgatggca gtttcaatca gttcttgccg ggtcttcaga
     9361 gcttcggctt tggttctttt tgccatgatt aattattcag gaaataaata tattcgacac
     9421 agggtgagaa aatagcgaag gttaatctat catctaatgt gtatttatac gagagggtaa
     9481 cattgagttg ctataaatcg tcaaataaac aatgtatatt atttacctaa aatacattcg
     9541 ccacatgaat atatatttca atttacgagg ttttaattct gcctctttca acccgcgtca
     9601 aaataaaaca gtagaatatt aatctttttt tgtgtttatg tgccttgaga cgccagtatt
     9661 cataactatt ccttacatcg acgaatgata atttgtagga tagcaaactg tatttttctt
     9721 tctgcgagtt aacgcgttgc ctttctgggt aaataacgcg cttttggttt tttgaggaat
     9781 agtaatgacg aaacatgcca ggtttttcct cctgccctcc tttattctga tctccgcggc
     9841 tttaatcgcc ggttgtaacg ataagggaga agagaaagct cacgtcggtg aaccgcaggt
     9901 taccgttcat attgtgaaaa cggccccgtt agaagttaag actgaattac caggccgcac
     9961 caatgcttac cgtatagccg aagttcgccc acaggttagc gggatcgtac tgaatcgtaa
    10021 tttcactgaa ggcagcgatg tacaagcagg ccagtccctg taccagatcg atcccgcgac
    10081 ctatcaggca aattataaca gcgcgaaagg cgaactggcg aaaagtgaag ccgccgccgc
    10141 catcgcgcat ttgacggtaa aacgttacgt tccgctcgtg ggtacgaaat acatcagcca
    10201 gcaggagtac gaccaggcca ttgctgatgc tcgccaggcc gatgccgccg tgattgccgc
    10261 aaaaggcaca gtcgaaagcg ctcgcatcaa tctcgcttat accaaagtga ctgcgccaat
    10321 tagcggacgt atcggcaaat cgaccgtgac cgaaggcgct cttgtcacta atgggcaaac
    10381 gactgaactg gcgactgtcc agcagctcga tcctatctac gttgatgtga cccaatccag
    10441 caacgatttt atgaggctga agcaatccgt agagcaagga aatttgcata aggaaaacgc
    10501 caccagcaac gtagagttgg tcatggaaaa cggtcaaacc tatcccctga aaggtacgct
    10561 gcaattctct gatgtgaccg ttgatgaaag caccggctcc ataaccctac gtgctgtctt
    10621 ccctaacccg caacatacgc ttttgccggg tatgtatgtg cgtgcacgga ttgatgaagg
    10681 cgtccaacct gacgccattc ttatcccgca acaaggcgtt agccgcacac cgcgtggtga
    10741 tgcaaccgtg ctgattgtta acgataaaag tcaggtggaa gcgcgccctg tcgttgccag
    10801 tcaggcgatt ggcgataaat ggttgattag cgaaggtctg aaatctggcg atcaagtcat
    10861 tgtcagcggc ctgcaaaaag cgcgtccggg agagcaggtt aaagccacta ccgatacccc
    10921 cgcagatact gcatcgaagt aaggtaatct gacatggcaa acttttttat tcgacgaccg
    10981 atatttgcat gggtgctggc cattattctg atgatggcgg gcgcactggc gatcctacaa
    11041 ttgcccgtcg ctcagtatcc aaccattgca ccgcctgcgg tttctgtttc ggcaaactat
    11101 ccaggtgctg atgcgcagac cgtgcaggat acggtgacgc aggttatcga acagaatatg
    11161 aatggtatcg ataacctgat gtatatgtcc tccaccaacg attccgccgg tagcgtgaca
    11221 attaccctta ccttccagtc cgggaccgat cctgatatcg cgcaagtgca ggtgcagaac
    11281 aaactccagc tcgccacgcc gttgctgccg caggaggttc agcagcaggg gatcagtgtt
    11341 gaaaagtcca gtagcagcta tttgatggtg gcgggttttg tctctgataa cccaggcacc
    11401 acacaggacg atatctcgga ctatgtggcc tctaacgtta aagatacgct tagccgtctg
    11461 aatggcgtcg gtgacgtaca gcttttcggc gcacagtatg cgatgcgtat ctggctggac
    11521 gccgatctgc taaacaaata taaactgaca ccggttgatg tgattaacca gttgaaggta
    11581 cagaacgatc agatcgctgc cggacagttg ggcggaacgc cagcgttacc agggcaacag
    11641 ttgaatgcct cgattattgc tcaaacgcgg ttaaaaaatc cggaagaatt cggcaaagtg
    11701 accctgcgcg taaacagtga cggctcggtg gtacgcctga aagatgtcgc acgggttgaa
    11761 cttggcggtg aaaactataa cgttatcgct cgtatcaacg gaaaaccggc ggcgggcctg
    11821 gggattaagc tggcaaccgg cgcgaatgct ctcgataccg cgaaagccat taaggcaaaa
    11881 ctggcggaat tacagccatt cttcccgcag ggaatgaagg ttctctaccc ttatgacacc
    11941 acgccattcg tccagctttc tattcacgaa gtggtaaaaa cgctgttcga agccattatg
    12001 ctggtgttcc tggtgatgta tctgttcttg cagaatatgc gagcaacgct gatccccacc
    12061 attgcggtac ccgtggtgtt gttagggacg tttgccatcc tcgccgcttt tggttactcc
    12121 atcaacacac taacgatgtt cgggatggtg cttgccatcg ggctgctcgt cgatgatgcg
    12181 atagtggtgg tggagaacgt cgagcgcgtg atgatggagg ataagctccc gccaaaagaa
    12241 gcgacggaaa aatcgatgtc gcaaattcag ggcgcactgg tgggtatcgc gatggtgctg
    12301 tcagcggtat ttattccgat ggcattcttc ggcggttcta ctggggcaat ttatcgccag
    12361 ttctctatca ccatcgtttc ggcaatggca ctttctgttc tggtggcatt gattcttacc
    12421 cctgcgttat gtgcaacgct gcttaaaccc gtctctgctg agcatcacga aaataagggc
    12481 ggtttcttcg gctggtttaa taccaccttc gatcatagcg ttaaccacta caccaacagc
    12541 gtcggcaaaa tcctcggttc cacaggacga tatttactga tctatgcgct gattgttgca
    12601 ggaatggtgg tgttgttttt acgtcttccg tcttccttct tacctgaaga ggatcagggt
    12661 gtctttctga ccatgattca gttacccgct ggcgcgacgc aagagcggac gcaaaaagtg
    12721 ttggatcaag tgacggatta ctatctgaag aacgagaaag cgaacgttga aagtgtcttt
    12781 acggttaacg gctttagctt cagcggccag gcacaaaacg ccggtatggc cttcgtcagt
    12841 ctgaaaccgt gggaagagcg taatggtgat gaaaacagtg cggaagcggt aatccatcgt
    12901 gccaaaatgg aattgggcaa gatccgcgac ggttttgtca ttccattcaa tatgccagcc
    12961 attgttgaac tgggcacggc aacgggtttc gactttgagt taattgatca ggctgggctg
    13021 ggtcacgatg ccctaaccca ggcccgtaac cagttgcttg gtatggcggc gcaacatcct
    13081 gccagcttag tcagcgtgcg tcctaatggc ctggaagaca ccgcgcagtt taaactggaa
    13141 gttgaccagg aaaaggcgca ggcattaagt gtttcacttt ctgacatcaa tcagaccatt
    13201 tcaacggcgt tgggtgggac ttacgttaac gacttcatcg accgtggtcg cgtgaaaaag
    13261 gtgtatgttc aggcggatgc caaattccgt atgctgccag aagatgtcga taaactttat
    13321 gtccgcagcg ccaacggcga aatggtgcca ttctcggcct ttaccacttc acattgggtg
    13381 tatggctctc cgcgactgga acgctacaac ggtctgccgt caatggagat tcagggggaa
    13441 gccgcgccag gaaccagttc cggcgatgcc atggcgttga tggaaaacct tgcgtcaaaa
    13501 ttacctgcgg gcattggtta tgactggacg ggtatgtcgt atcaggaacg cttatcggga
    13561 aaccaggctc ccgctctagt agcaatttcc tttgtggttg ttttcctgtg ccttgctgca
    13621 ctctatgaaa gctggtcaat tcctgtctcg gttatgttgg tcgtgccgtt agggattgtc
    13681 ggcgtgctgc tggcggcgac actctttaat caaaaaaatg acgtctactt tatggtgggc
    13741 ttgctaacga caattggctt gtcggccaaa aacgctattt tgattgttga gttcgctaaa
    13801 gatctcatgg agaaagaggg taaaggtgtt gttgaagcga cactaatggc agtacgtatg
    13861 cgtctgcgtc ctatcctgat gacctctctc gcctttattc tcggcgtatt accgctagct
    13921 atcagtaacg gtgcaggcag tggcgcgcag aacgcagtgg gtatcggggt aatgggcgga
    13981 atggtctctg caacgttgct ggcaatcttc ttcctaccgg tgttctttgt ggtgatccgc
    14041 cgttgcttta aaggataaat cagaaacata aaggcacttt cgggtgcctt tattattttc
    14101 agtgaaaccc ataaaaatta ataagatatt cttccactca ctctttaaaa actttctata
    14161 gttcccgctc ccttcactat ttttacaatt cacataattt gtcattattc attccgcaat
    14221 atttataacc tccggtgata aaatggcatt cagctcgtta ataagagagt taacttatta
    14281 agcgttagcg tttattactg aggtaacacc atgaaaagat taattcctgt ggcattgctc
    14341 accgcattgc tggcgggctg tgctcacgat tcgccctgcg ttccggttta tgacgatcag
    14401 ggttgtctgg ttcacaccaa tacctgtatg aaaggcacga ctcaggataa ctgggaaacg
    14461 gctggggcta ttgccggcgg ggcagcggct gttgctggcc tgacgatggg gatcatcgct
    14521 atgtcgaaat aactgaacag ggcgcggagt ttcgcgctct gtttaacgat acagtatctt
    14581 tacataagat aaaatattgc tatattctgc tggctaattc tctgctgatt tagcatgtta
    14641 tatcgcgtta tttcgctgct ttcgaaataa cgcatcaatt taactttcac acttcccacc
    14701 agaaatatac tcacgctaac aatcattatt ctgcttagta aaacacgcgt aaatattttg
    14761 cgccaaaatg tggcgcatgt ttcattttcg caccattgcg gggcgctgtt tttattattt
    14821 cctgtctaca ctcagcttat tgcgacgcga aatcgtgcgc aaaagctggc actacttttg
    14881 cttataagag gatggccaca gacaggtaaa agacgtttcc caaacgtcct ataacgataa
    14941 ttttcgcctc acaggaagca ttatgaaaaa gatgatgata gccacactgg ctgccgccag
    15001 cgtgctgctt gccgttgcaa atcaggcgca tgctggcgcg acgcttgatg ccgttcagaa
    15061 aaaaggtttt gtgcaatgcg ggatcagtga tggattactt gggttctctt atgccgatgc
    15121 agacggtaag ttttcaggta ttgatgttga tgttgatgtt gatgttgatg tttgtcgtag
    15181 tgttgccgct gctgtatttg gtgacgacac gaaagtgaaa tataccccac tcacagcaaa
    15241 agaacgcttc acggctttac agtcagggga agtggatttg ctctcccgta atacgacctg
    15301 gacttcatct cgcgacgccg ggatgggaat ggcatttact ggcgtcactt attacgacgg
    15361 cattggcttc ctgacgcacg ataaagcggg gctaaaaagc gcgaaagaac tggatggcgc
    15421 taccgtctgt attcaggcgg gtactgatac cgaactcaac gttgccgact atttcaaggc
    15481 aaacaatatg aagtacacac cggtgacttt cgatcgctct gacgaatcag cgaaggcact
    15541 ggaatctggt cgctgcgata cgctggcctc ggatcagtcg caactgtatg ccctgcgcat
    15601 caaattaagc aacccagctg aatggattgt cttaccggag gttatctcta aagaaccgct
    15661 tgggccggta gttcgtcgtg gcgatgatga atggttctcg attgtacgct ggacgctttt
    15721 cgccatgctg aatgctgaag agatgggcat caattcccag aacgtcgatg aaaaagcggc
    15781 taatccagca acgcctgata tggcacatct gctgggtaaa gaaggcgatt acggcaagga
    15841 tctgaagttg gataataaat gggcctataa catcatcaaa caggtgggta actactcgga
    15901 aattttcgag cgtaacgtag gttcagaaag cccgctgaaa attaaacgtg ggcaaaataa
    15961 tctctggaat aacggcggta ttcagtacgc gccgcccgtg cgttaagtcg gtgtgaggta
    16021 acgagcgtcg ctacggcggc gctctcttca gcgttatgct ttccgaggtt tctatgtctc
    16081 atcgccgctc aaccgttaaa ggctcactct cctttgccaa ccctacggtt cgtgcctggt
    16141 tattccagat ccttgccgtt gttgctgttg tcggaattgt tggttggtta tttcacaaca
    16201 ctgtaacgaa tctcagtaat cgtggcatta cttcaggttt tgcctttctg gatcgcggcg
    16261 ctggcttcgg tattgtccag catttgatcg attaccagca gggcgacacc tacggacgcg
    16321 tttttattgt cggcttactc aatacgctac tggtttctgc attgtgtatt gtgttcgctt
    16381 ctgttttagg cttctttatc ggtctgacga gactttcgga taactgactg ctacgaaagc
    16441 tatccacaat ttatattgag atcttccgta atattccccc gctgctgcaa atcttcttct
    16501 ggtactttgc cgtgttgcgc aatttgcccg gaccacgcgc caggctgtga gcgcgtttga
    16561 tctggccttt ttgagcaatc gtgggcttta tattccgtca ccgcagctgg gagacggatt
    16621 tattgcgttt atcctagctg ttgttatggc tatagtcctt tctgttgggc tattccgctt
    16681 taataaaaca taccagataa agaccggaca actgcgccgc acctggccgt tcgccgcagt
    16741 gttgatcatc ggtttgcctt tactggcgca atggcttttt ggcgcagcac tacactggga
    16801 tgtcccagcc ctacgaggct ttaacttccg cggcggaatg gttttaattc ctgaactggc
    16861 agccttaacg ctggcacttt cggtttatac ctctgcattt atcgccgaga ttatccgcgc
    16921 tgggatccag gcagtgcctt atggtcaaca tgaagcggct cggtcactgg ggttacgcta
    16981 cgccaggtca ttattcccca ggcactgcga gtgattattc cgccgttaac cagccagtat
    17041 ctcaacatcg tcaaaaactc ctctcttgcc gccgctattg gctatcccga tatggtttcg
    17101 ctgtttgctg gcaccgtgct gaaccagacg gggcaagcca tcgagacgat agccatgacc
    17161 atgtcggtct atctgattat cagcctgact atctcgctgc tgatgaatat ctataaccgc
    17221 cgcatcgcga tcgttgaacg ctaaggagtc atgatgacaa aagtattgct gtctcagccc
    17281 tcgcgcccag cgagccataa ctcaagccgc gcgatggtgt gggtgcgaaa aaatctgttc
    17341 tccagctgga gcaatagcct gctgactatt ggctgcatat ggttgatgtg ggaactgatc
    17401 ccaccgttgc tgaactgggc atttttgcag gctaactggg ttggctcaac gcgtgccgac
    17461 tgcacaaaag ccggtgcctg ttgggtcttc atccacgaac gatttggtca gtttatgtat
    17521 ggactttacc cacacgacca acgctggcga attaacctag tattactgat tggccttgtg
    17581 tcaatcgcac caatgttctg gaaaatactc ccgcatcgcg gtcgctatat tgcggtctgg
    17641 gcggtgattt acccactgat tgtctggtgg ctgatgtatg gcggtttttt tggtcttgag
    17701 cgggttgaaa cccggcaatg gggcgggctg acgctaactt taattattgc atccgttggg
    17761 attgcggggg cgctaccgtg ggggatctta ctggcgttag gtcgccgctc ccatatgccg
    17821 attgtgcgta tcttatcggt catttttatc gagttctggc gcggcgtacc gctgattacc
    17881 gtattgttta tgtcttcggt catgctgccg ttgtttatgg cagaaggcac cagtatcgac
    17941 aaactgatcc gcgcgctggt tggcgtgatc ctgtttcagt cagcatatgt tgcggaagtc
    18001 gtgcgaggcg gattacaggc cctacctaaa gggcaatatg aagcggcaga gtcgctggcg
    18061 ttggggtact ggaaaactca ggggctggtt attctgccac aggcgttgaa gctggtaatt
    18121 cctgggctgg taaataccat catcgcactc ttcaaagata ccagcctggt gatcatcatc
    18181 ggcttgttcg atcttttcag tagcgttcag caggcaaccg ttgatcccgc ctggttgggt
    18241 atgtcgacgg aagggtatgt tttcgccgcg ctgatctact ggatcttttg tttcagcatg
    18301 tcacgttata gccaacatct ggaaaaacgt tttaacaccg ggcgtacacc gcattgagga
    18361 cactatgagc caaattttac tgcaacctgc taacgcgatg attacgctgg aaaacgtcca
    18421 taaatggtat ggacaattcc atgttttgaa aaatataaat ttaaccgtgc aaccgggaga
    18481 acggatcgtt ctgtgtggcc cttctggttc cggtaaatcg acaaccattc gttgtattaa
    18541 tcatctggaa gaacatcaac agggacggat tgtcgtagat ggcatcgaac ttaatgaaga
    18601 tatccgcaat attgagcgcg tcaggcaaga agtgggaatg gtatttcagc atttcaatct
    18661 cttccctcat ctgaccgttt tacagaactg taccctggca ccgatttggg tacgcaagat
    18721 gcctaagaaa gaggctgaag ctctggcgat gcattacctt gagcgggtga gaattgccga
    18781 acatgcgcat aagtttcccg gacagatttc aggtggtcag cagcaacgcg ttgccattgc
    18841 gcgttcgctg tgtatgaagc cgaaaattat gttgtttgat gaaccaacgt cggcctcgat
    18901 ccggagatgg tgaaagaggt gctggatacg atgattgggc tggcgcagtc gggtatgaca
    18961 atgttgtgtg taacacatga gatggggttt gcacgtaccg tcgctgaccg ggtaattttt
    19021 atggatcgtg gggagatagt ggaacaagct gcacctgatg aattttttac gcatcctaaa
    19081 tcagagcgta cgagggcatt tttatcgcag gtaatccatt aattgaatgt tagttcgaaa
    19141 agcaaaaagg ccatcctttc ggatggcctt tttgcttgat ttgatgtctg gcagtttatg
    19201 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa tccgctcccg
    19261 gcggatttgt cctactcgag agagtgttca ccgacaaaca acagataaaa caaaaggctc
    19321 agtcggaaga ctgggccttt tgttttattt gatgtctggc agttccctac tctcgcatgg
    19381 gaagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca
    19441 ggtgggacca ccgcgctact gccgccagac aaattctttt ctaatttgcc gaactttaac
    19501 ctaaaaagtg gtgctgatac ccagagtcga actggggacc tcacccttac caagggtgcg
    19561 ctctaccaac tgagccatat cagcacgct
//
LOCUS       DAJTSL010000069        19489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTSL010000069 DAJTSL010000000
VERSION     DAJTSL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.069"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1230
                     /locus_tag="OO433_002188"
     CDS             <1..1230
                     /locus_tag="OO433_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6050656.1"
                     /translation="FFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSS
                     LESAWKYIIICTVDVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLML
                     LAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIIC
                     QAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPL
                     GIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALA
                     LAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQA
                     VNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTWH
                     DFLPSGTASVSEKHSER"
     gene            1220..2935
                     /gene="hyfG"
                     /locus_tag="OO433_002189"
     CDS             1220..2935
                     /gene="hyfG"
                     /locus_tag="OO433_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR6050657.1"
                     /translation="MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            2945..3484
                     /gene="hyfH"
                     /locus_tag="OO433_002190"
     CDS             2945..3484
                     /gene="hyfH"
                     /locus_tag="OO433_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6050658.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            3481..4239
                     /gene="hyfI"
                     /locus_tag="OO433_002191"
     CDS             3481..4239
                     /gene="hyfI"
                     /locus_tag="OO433_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6050659.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            4232..4645
                     /gene="hyfJ"
                     /locus_tag="OO433_002192"
     CDS             4232..4645
                     /gene="hyfJ"
                     /locus_tag="OO433_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6050660.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHRSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTENEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            4675..6687
                     /gene="hyfR"
                     /locus_tag="OO433_002193"
                     /pseudo
     CDS             4675..6687
                     /gene="hyfR"
                     /locus_tag="OO433_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            6709..7554
                     /locus_tag="OO433_002194"
                     /pseudo
     CDS             6709..7554
                     /locus_tag="OO433_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(7592..8653)
                     /locus_tag="OO433_002195"
     CDS             complement(7592..8653)
                     /locus_tag="OO433_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6050661.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            8866..10329
                     /gene="bepA"
                     /locus_tag="OO433_002196"
     CDS             8866..10329
                     /gene="bepA"
                     /locus_tag="OO433_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6050662.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHLTQRHLARAMEDQQRSTPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            10350..10709
                     /gene="arsC"
                     /locus_tag="OO433_002197"
     CDS             10350..10709
                     /gene="arsC"
                     /locus_tag="OO433_002197"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6050663.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLANSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(10847..11593)
                     /gene="hda"
                     /locus_tag="OO433_002198"
     CDS             complement(10847..11593)
                     /gene="hda"
                     /locus_tag="OO433_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6050664.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11643..12932)
                     /gene="uraA"
                     /locus_tag="OO433_002199"
     CDS             complement(11643..12932)
                     /gene="uraA"
                     /locus_tag="OO433_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6050665.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEEADITDK"
     gene            complement(13018..13644)
                     /gene="upp"
                     /locus_tag="OO433_002200"
     CDS             complement(13018..13644)
                     /gene="upp"
                     /locus_tag="OO433_002200"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6050666.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            13968..15005
                     /gene="purM"
                     /locus_tag="OO433_002201"
     CDS             13968..15005
                     /gene="purM"
                     /locus_tag="OO433_002201"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6050667.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            15005..15643
                     /gene="purN"
                     /locus_tag="OO433_002202"
     CDS             15005..15643
                     /gene="purN"
                     /locus_tag="OO433_002202"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6050668.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKIHENAAWLDGQRLPPQGYAADE"
     gene            15815..17881
                     /gene="ppk1"
                     /locus_tag="OO433_002203"
     CDS             15815..17881
                     /gene="ppk1"
                     /locus_tag="OO433_002203"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6050669.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNPEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPHLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            17886..19427
                     /gene="ppx"
                     /locus_tag="OO433_002204"
     CDS             17886..19427
                     /gene="ppx"
                     /locus_tag="OO433_002204"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6050670.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRVEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
BASE COUNT         4703 a         5094 c         5093 g         4599 t
ORIGIN      
        1 ttcttccatc tgtttttgtt caccatgctg ctggttgtta ccagcaataa cctgattgtg
       61 atgtgggcgg cgatcgaagc caccacctta agctcggcgt ttctggtagg catttacggt
      121 cagcgttcat cgctggaatc agcatggaag tacatcatta tttgtactgt tgatgtcgct
      181 tttggtctgt tcggtaccgt gctggtatac gccaacgccg ccagcgttat gccgcaggca
      241 gaaatggcga tattctggag cgaggttctt aagcaatcgt ccttgcttga cccaacatta
      301 atgctgttgg cctttgtgtt tgtgctaatt ggctttggta ccaaaaccgg gctatttccc
      361 atgcacgcct ggctgccgga tgctcacagt gaagcgccga gtccggtcag cgccctgctc
      421 tccgccgtat tgctgaactg cgcgctgttg gtgctgattc gctattacat cattatttgc
      481 caagccatcg gcagcgattt ccccaaccgg ttgttgctca tcttcggcat gttgtcggtt
      541 gccgtggcgg catttttcat tctggtacag cgggacatta agcgtctgct ggcgtactcc
      601 agcgtggaga acatggggct ggtcgcggtg gcgctaggca ttggcgggcc gctgggaatt
      661 tttgccgcgc tgctgcacac cttaaaccac agtctggcaa aaacgctgct gttctgcggt
      721 tccggcaatg tactgctcaa gtacggcacg cgcgatctca acgtcgtctg tgggatgctc
      781 aaaatcatgc catttaccgc cgtgctgttt ggcggcggtg cgctggcgct ggcagggatg
      841 ccgcccttca acatttttct tagcgaattt atgaccgtta ccgcaggact ggcacgtaat
      901 cacctgctga ttatcgtcct gctgttattg ctgttaacgc tggtgctggc gggcctagta
      961 cggatggctg cgcgggtgtt aatggcgaaa ccgccgcagg ccgttaaccg gggtgatctc
     1021 ggctggttga ccacctcgcc aatggtgatt ctgctggtca tgatgctggc gatgggaacg
     1081 catattccac aacctgtcat caggatcctg gcgggcgctt ccactatagt cctctcaggg
     1141 acgcacgacc tgcctgcaca acgtagcacc tggcatgatt ttttgccttc aggcaccgca
     1201 tctgtttcgg agaaacacag tgaacgttaa ttcatcgtca aatagtggcg aagcgattct
     1261 cgccgccctg aaaacgcagt tccccggcgc ggtactggat gaagagcgac aaacgcctga
     1321 acaggtcacc attacggtga aaatcaatct gctgcctgac gttgtacatt atctttatta
     1381 tcaacatgat ggctggcttc cagtcctgtt tggcaacgac gagcggacac ttaacggtca
     1441 ttacgcggtt tattatgccc tttcaatgga aggggccgaa aaatgctgga tcgtggtgaa
     1501 ggcactggtc gatgccgaca gtcgggagtt tccgtcagtc acaccgcgcg tccctgccgc
     1561 ggtctggggc gagcgagaaa ttcgcgatat gtacgggctg attccggttg gcctgccgga
     1621 tcagcgtcgc ctggtgttgc ccgatgactg gccggaagat atgcatccgc tgcacaaaga
     1681 tgcgatggat tatcgactgc gccctgaacc gacgacagat tccgaaacgt atgcgtttat
     1741 caatgagggc aacagcgatg cgcgggtgat ccctgtcggc ccgctgcata tcacctccga
     1801 tgaaccgggt cacttccgct tgtttgtgga tggcgagcaa attgtcgatg ctgattaccg
     1861 cctgttttat gtccatcgag gcatggagaa actggcagaa acgcggatgg gctacaacga
     1921 agtgaccttc ttatcggacc gcgtgtgtgg gatttgcggt tttgcccaca gtgtggccta
     1981 taccaactcg gttgaaaatg cactggggat tgaggtgccg caacgagcgc ataccattcg
     2041 ctcgattctg ctggaagtcg aacggctaca cagtcatttg ctcaaccttg gcctctcctg
     2101 ccattttgtt ggttttgata ccggctttat gcaatttttc cgcgtgcggg aaaagtcgat
     2161 gacgatggcg gaattgctga ccgggtcgcg taaaacctac ggtctgaatc tgattggtgg
     2221 tgttcgccgc gatattctca aagagcaacg tctgcaaacg ctgaaactgg tgcgcgagat
     2281 gcgcgccgac gtgtcggagc tggtagagat gctgcttgct acgccgaata tggaacaacg
     2341 cactcagggc attggcattc tcgaccgaca aatcgcccgt gattatagcc ctgtagggcc
     2401 gctgatccgc ggcagtggtt ttgcccgtga tttgcgcttt gatcacccct acgccgacta
     2461 cggcaatatt ccaaaaacac tgtttacctt caccggcggc gatgttttct cccgcgtgat
     2521 ggtccgtgtc aaagagacgt ttgattcgct ggcaatgctg gaatttgccc tcgacaacat
     2581 gccggatacc ccactgctga ccgaaggctt tagctataaa cctcacgcat tcgcgctggg
     2641 ctttgttgaa gcgccacgcg gtgaagacgt gcactggagc atgctcggtg ataaccaaaa
     2701 attgttccgc tggcgctgcc gtgccgccac ctacgccaac tggccggtgt tgcgttacat
     2761 gctgcgcggc aataccgttt ctgacgcacc gctgattatc ggtagccttg atccctgcta
     2821 ctcctgtacc gaccgtgtga cgctggtaga tgtgcgcaag cgccagtcaa aaaccgtgcc
     2881 gtataaagag atcgaacgct acggcattga tcgtaaccgt tcgccgctga agtaaggaca
     2941 gaagatgctg aagttactga aaactattat gcgcgccgga accgcgacgg tgaaatatcc
     3001 cttcgcgcca ctggaggtca gcccaggctt tcgcgggaaa ccggacctga tgcccagcca
     3061 atgtattgcc tgcggcgcct gtgcttgtcc ggcaaatgcg ctgactatcc agaccgacga
     3121 ccagcaaaat tcgcgcacct ggcagctcta tctggggcgt tgtatttact gcggacgttg
     3181 tgaagaagtg tgcccgacca gagccatcca gcttaccaat aactttgaac tgaccgtcac
     3241 caataaagcc gatctctata cccgcgcgac gttccatcta caacgttgca gccgttgcga
     3301 acgcccgttt gccccgcaaa aaaccgtcgc actggctgct gaattgttgg cacagcaaca
     3361 aaatgcgcca caaaaccgcg aaatgttgcg ggcgcaagcg agcgtctgcc cggagtgcaa
     3421 acaacgcgcg acgctgatca acgacgatac agatgtaccg ctggtggcta aggagcagct
     3481 atgagtccag tgcttacaca acatgtcagc cagcccatca cgctggatga gcaaacgcaa
     3541 aagatgaagc ggcatttgct acaggatatc cgtcgctcag cctacgttta tcgcgtcgat
     3601 tgcggcggct gcaacgcctg tgaaatcgaa atttttgctg ccatcacccc cgtattcgac
     3661 gcagaacgtt ttggcattaa ggttgtttca tcaccgcgtc acgccgatat tttgttattt
     3721 actggcgcag tcacccgggc gatgcgtatg cctgcacttc gggcgtatga gtctgccccc
     3781 gatcataaaa tctgtgtttc ctacggcgcg tgcggtgtcg gcggcggtat tttccacgat
     3841 ctctacagcg tctggggcgg cagcgacacc attgtcccca ttgatgtttg gatccccggc
     3901 tgcccgccaa caccggccgc caccattcac ggtttcgccg tggcgctcgg tttgctgcaa
     3961 cagaagattc acgctgtgga ttatcgcgat cccaccgggg tgactatgca accgttgtgg
     4021 ccacagatcc cgccatcaca gcgtatcgcc attgagcgag aagcgcggcg gctggcgggc
     4081 tatcgtcagg ggcgagaaat ttgcgatcgg ctcctgcgcc atttaagcga cgatcctacc
     4141 ggaaatcggg ttaacacctg gttgcgcgat gccgacgatc cacgtctcaa tagtatcgtt
     4201 cagcaactct ttcgcgtact ccgggggtta catgactgaa gagtgcgggg aaattgtttt
     4261 ctggacgctg cgaaaaaagt ttgtcgccag tagcgacgag atgccggaac accgctctca
     4321 ggtaatgtat tactcgctgg ctatcggcca tcacgttggc gtgattgatt gtctgaatgt
     4381 cgccttccgc tgcccactga cggaaaacga agattggctt gcacaggtcg aagaggagca
     4441 agcccgacgt aagatgctgg gggtgatgac ctttggtgag attgttattg acgccagcca
     4501 caccgccctg ttgacccggg cattcgcgct actggcagat gacgcgacgt ctgtgtggca
     4561 ggcgcgtagc attcaattca ttcatctgtt ggatgaaatt gtgcaggaac cggccatcta
     4621 tctgatggcc agaaaaattg cgtgagaagg atttctcatt aataaggact gttgatggct
     4681 atgtcagacg aggcgatgtt tgccccgcca caaggaataa caattgaagc ggtaaacgga
     4741 atgctcgcgg agcggttagc acagaaacac ggtaaggcgt ctttattacg cgccttcatc
     4801 ccgatgccgc cgccgttcag cccggtacaa cttattgaac tgcatgttat caaaagcaac
     4861 ttctattacc gctaccatga tgatggcagc gatgtgacgg caacaacaga gtatcagggc
     4921 gagatggtcg attattcgcg tcacgccgtc cttctcggca gtagtggaat ggcggagcta
     4981 cgctttattc gtacccacgg cagccgtttt actccctagg attgcacact gtttaactgg
     5041 ctggcacgca ttatcactcc ggttctgcaa tcatggctca atgatgaaga acaacaggtg
     5101 gcgctgcgtt tgctggagaa agatcgcgat catcatcggg tactggttga tattactaat
     5161 gcagtgctgt cacatcttga tctcgacgat ctgatcgctg acgtcgctcg tgagatccat
     5221 cattttttcg gtctggcttc agtcagtatg gtactgggcg agcatcgaaa gaacgagaag
     5281 tttagcctgt ggtgcagcga tctttctgcc tcacattgtg cgtgtctgcc acgcaatatg
     5341 cctggcgaca gtgtattgct gacacaaacg ctacaaaccc gacaaccgac cttgacgcac
     5401 cgtgcagacg atctgtttct ctggcaacgc gacccgttat tactcttact tgcatctaac
     5461 ggctgcgaat ctgcgctcct tataccgctt acctttggca accatacacc gggtgcattg
     5521 ttgctggcgc atacctcttc cactctcttt agtgaggaaa actgccagct actacaacac
     5581 atagccgatc gcatcgctat tgccgttggc aatgccgatg cctggcgtag catgaccgat
     5641 ttgcaggaaa gtttgcagca agaaaactac cagcttagcg agcagctcct ttcgaatctg
     5701 ggcatcggtg acattatcta tcaaagccag gcaatggaag acctgctcca gcaggtagat
     5761 attgtagcga agagcgacag tacggtgttg atttgtggtg aaaccggaac cggcaaagag
     5821 gtgatcgcca gagcgctcca tcaacttagc acgcgacgcg acaaaccgct ggtcaaaatc
     5881 aactgcgctg ccatccccgc cagtcttctg gaaagtgagt tattcggtca tgacaaaggg
     5941 gcgtttactg gtgcgattaa tacacatcgt ggtcgttttg aaattgccga tggcggcacg
     6001 ttgtttctcg atgaaattgg cgatctgccg ttagaacttc agcctaaact gctgcgcgta
     6061 ttgcaggaac gggagattga gcgtctcggc gggagtagaa cgatcccggt gaatgtcaga
     6121 gtcattgccg ccaccaaccg tgatttgtgg caaatggttg aagatcgcca gtttcgcagc
     6181 aatctctttt atcgcctgaa tgtcttccca ttggaattgc cgccgctacg cgaccgtccg
     6241 gaagatatcc ctcttttagc aaaacatttc acgcaaaaaa tggcgcgcca tatgaatcgc
     6301 gcaattgacg ccatcccgac cgaggcgcta cgccagttga tgtcgtggga ctggccgggc
     6361 aacgtgcacg agctggaaaa cgtgattgag cgagcggtac tgctgactcg cggtaacagt
     6421 ctgaatttac atcttaatgt ccgacaaagc cgtttactgc cgacgctaaa tgaagattca
     6481 gcgcttcgca gttcaatggc gcagttgctg cacccgacga cgccagagaa tgacgaagaa
     6541 gaacgtcagc gcattgttca ggtattgcga gaaaccaatg gcattgttgc cgggccccgt
     6601 ggcgcggcga cacgattagg gatgaagcgc accacgctgc tgtcacgaat gcagcgtctg
     6661 gggatctcgg ttcgcgaggt gttgtaatct gcttttgcag gagtatgcat gagaaacaaa
     6721 ctctctttcg acttgcagtt gagcgccaga aaagcggcaa tcgctgaacg gattgccgtc
     6781 cataaaattg cccgcagtaa agtgtcggtc tttttaatgg cgatgtccgc tggcgtgttt
     6841 atggcgatcg gatttacttt ttacctttcc gttatcgccg atgccccgtc ttcacaggca
     6901 ttaacccatc tggtgggcgg cctttgcttt acactcggct ttattttgct ggcggtttgc
     6961 ggcacagcct gttcacctcg tcggtaatga cggtgatagc aaaaagtcgg ggcgttatta
     7021 gttggcgaac ttggctgatt aacgcacttc tggtggcctg tggtaatctg gcagttattg
     7081 cctgtttcag tttgttaatc tggttttccg ggctggtgat gagtgaaaac gcgatgtggg
     7141 gagtcgcggt tttacactgc gccgagggca aaatgcatca tacatttact gaatctgtca
     7201 gcctcggcat tatgtgcaat ctgatggttt gcctggcgct gtggatgagt tattgcgggc
     7261 gttcgttatg cgacaaaatc gtcgccatga ttttgcccat caccctgttt gtcgccagtg
     7321 gctttgagca ctgtatcgcc aatttgtttg tgattccgtt cgccattgcc attcgccatt
     7381 tcgccccccc ccttctggca actggcgcac agtagcgcag acaattttcc ggcactgacg
     7441 gtcagccatt ttattaccgc caatctgctc ccggtgatgc tgggtaatat tatcggcggt
     7501 gcagtgctgg tgagtatgtg ttatcgggct atttatttac gtcaggaatc ctgaaaaatc
     7561 agcccggcga aacagttcgt cgggctgaag attattcttg cgcgatttgc ccatcgggcc
     7621 aggcgtggat cacggctttg atcagcgtcg ccaatggaat ggcgaagaat acgccccaga
     7681 atccccacaa accaccgaag atcaccaccg ataaaataat caccagcgga tgcaggttaa
     7741 ccgcttcgga gaacaacacc ggtactagca ggttgccgtc cagcgcctga ataatcagat
     7801 acaccgcgaa gcagctccag aattccgtgc ctgcgccaaa ctggaatagc gccacgccaa
     7861 ccaccggaat ggtcaccaca aatgcgccaa tgtacggaat aagaaccgag aagccgacca
     7921 gcaccgccag cagcagcgaa tagttcagcc caaagagcaa gaaccccagc caggtggcga
     7981 tccccaccac gatcatctcc agcactttgc cgcggatata gttggtgatt tgttgattca
     8041 tctccttcca cacctgtcct gccagtccac ggttgcgcgg cagcacccga cgaacggcgt
     8101 tcagcatctg ctctttgtct ttcagcagga agaagaccat caatggcacc agcaccagat
     8161 agacggcaat ggtcagcaaa ccgaccagcg aggcgaggga aattttcacc accgaatcgc
     8221 ccatggtcag catccgactg cgcatatttt cggccattgc atcaataatg cccgcgtcca
     8281 ttaacgccgg atagcggcgc ggcaacgtgg cggcaaagtc agaaagctta ttgagcatcc
     8341 ccggcatatc gcggattaag tagatgccct gttgccaggc gataggcagt accacgaacg
     8401 ccatcagtag caatataccg acaaaaacca ccaatacaat cgacgtcgcc cagcggcggg
     8461 agcagccaat agattgcagg cgcacggttg gccattccag caaataggcc agcacaatag
     8521 ccaccagcaa cggagcaagc aggccactaa agaaaaagat aatgccaaat ccggcaacta
     8581 aaataaccag caaggcaatc gcttccgggt cgctaaaacg gcggcgatac cattgcatca
     8641 acatttcgag cataaaaacc ttccctgaac ctcaagagcg ggattgcgat ccgcaattgt
     8701 atcgaaatgt cacaaaaaag acttcgcttt ttatgacgga ttcaggaaac tgaaaagtca
     8761 tttgagtggg ctaatcttcg ccgttacact caaaggcggc gcggtgggaa cgatatttca
     8821 cagtatcggt caaatgacta tcttcagaaa tacaggatag aggttatgtt caggcagttg
     8881 aaaaaaaacc tggttgcaac cctcattgct gctatgacca ttggtcaggt agccccggcg
     8941 tttgccgaca acgcagacac cttgccggat atgggaacct ccgcaggaag cacgctttcc
     9001 attggtcagg aaatgcagat gggcgactat tatgtccgcc agctacgcgg cagcgcgccg
     9061 ttaattaatg acccgctgtt aacgcaatat attaattcgc tggggatgcg tctggtttcg
     9121 catgccaatt cggttaagac accgtttcat ttttttctaa tcaacaacga cgaaattaac
     9181 gcctttgctt tctttggcgg caacgtggtg ctgcactctg ccctgttccg ttattccgat
     9241 aacgaaagtc aactggcttc agttatggcg cacgaaatct cccacctcac ccaacgtcac
     9301 ctggcgcgag cgatggaaga tcagcagcgc agcacgccgc tgacctgggt cggcgcgtta
     9361 ggttctattt tactggcgat ggccagtccg caggcgggga tggcggcgct gaccggtaca
     9421 ctggcgggaa cgcgtcaggg gatgatcagt ttcacccagc aaaatgaaca ggaagcggac
     9481 cgcattggta ttcaggtgct gcaacgctcg ggattcgatc cgcaggcgat gccaaccttc
     9541 ctcgaaaaat tactcgatca ggcgcgttac tcctcgcgcc cgccggaaat tttattgact
     9601 cacccgttgc cggaaagtcg tctggcagat gcccgcaacc gtgctaatca gatgcgaccg
     9661 atggtggtgc agtcgtcgga agatttctat ctggcgaaag cgcgcacact ggggatgtat
     9721 aattccggac gtaaccagct caccagtgat ttgctggatg aatgggcgaa aggaaacgtt
     9781 cgtcagcaac gagcggcgca atatggtcgt gctttacagg cgatggaagc caataaatac
     9841 gacgaggcgc gtaaaacgct gcaaccgtta ctggcggcag aacctggcaa cgcatggtat
     9901 ctcgatctgg ctaccgatat cgatcttggg caaaacaaag ccaatgaggc aatcaatcgc
     9961 ctgaaaaatg cccgtgattt gcgcaccaat ccggtgttgc agctcaacct ggcaaacgct
    10021 tatctgcaag gcggtcaacc acaagaagcg gccaatattc tcaatcgcta cacctttaat
    10081 aataaagatg acagcaacgg ctgggattta ctggcacagg cggaagccgc gctaaataac
    10141 cgcgatcagg agctggctgc gcgagcagaa ggttatgcgc tcgccggacg actcgatcag
    10201 gccatttcgc tgttgagtag cgccagttcg caggtgaaat taggcagcct gcaacaagcg
    10261 cgttacgatg cgcgcatcga ccagttgcgc cagctgcagg aacgctttaa gccttatacc
    10321 aagatgtaat tcgcattaaa ggaagaacaa tgaccaaaca ggtaaaaatc taccataacc
    10381 cacgctgttc aaagagccgg gaaacgctga acctgctgaa agaaaacggc gtggagccag
    10441 aagtggtact ctaccttgag acacccgccg atgcggcaac gctgcgcgat ttgctgaaaa
    10501 tgctggggat gaatagcgcc cgtgaattga tgcgccagaa agaggatctt tataaagaac
    10561 tgaacctggc taacagttcg cttagcgaag aggcgctgat tcaggcaatg gtggataatc
    10621 cgaagctgat ggagcgcccg attgtggtcg cgaatggcaa agcgcggatt ggtcgtccac
    10681 cggagcaggt actggaaatc gtgggttaag atgtgctcca tcgctagttg aagcacattg
    10741 ccggatgcga cgcttgccat gtcttatccg gcctacgaat actatggatt aacttcgcgt
    10801 agttcggata aggcgttcgc gccgcatccg acaataaaca ccttatctac aacttcagaa
    10861 tttctttcac aaacggaatg gtcagcttac gttgtgcggt aatcgacgca cgatccaact
    10921 gatccaacgt cataaatagc gtgcgcattt ctctgtcgag ccgcttcagc aagaaacgcc
    10981 ccacatcttc cggcagttca aaaccacgca aacgcgcgcg taactgtagc gcctgcaact
    11041 tatcttcatc agaaagtggc tgcaatttgt agatctgccc ccagtcgagt cgcgacgcga
    11101 gatccggtaa tcccagattc aactgccgcg gtggacgatc gccggtgatc aacaaccgtg
    11161 ttttgcccga ttccagaatt cgattgtaga gatcgaaaat cgccatctcc cacaactcat
    11221 cgcctgcaat acactcaatg ttgtcgatac aaaccagcga caaatgctcc ataccgtcga
    11281 gcacttccgg aacaaaccaa gtgcgtttat ccagcgggac atagcccacc gcatcgccac
    11341 gctgcgacaa ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg ccttcgcgtg
    11401 cccagagata gatgtaaccg ctatgttcct gacgcagcac gttttgcagc gcggccagta
    11461 aagaggagtt atcccccggc cagaaacttg caaaggtttc gtcgtcagga agataaagtg
    11521 gcaaagagag ctgtgccggt gtgttcagag atacctcaac caggatttca caaaatcgcg
    11581 agaagtttac cacagaatcc catgatgttt gaaccgggca gcaacactgc ccggtcggta
    11641 cattatttgt ctgttatatc cgcctcttct gcgtccagca ccacttcttc agggcgcagc
    11701 acactgatca gtttgaaaat caaacttaag ccgataccga cgatggtcgc cagcgccata
    11761 ccttttaact ccgccgcgcc gatgtttacc ttcgcgccac tgacgccgat gatcaaaatc
    11821 acggaagtca ggatcaggtt ctgtgctttg ttgtaatcca ctttcgattc gatcaaaaca
    11881 cgaataccgg aagcaccgat gacaccataa agcagcagcg aaacgccgcc catcaccggc
    11941 aatgggatca tctggatagc ggcagccagt ttaccgacgc aggaaagcag gatagcgaaa
    12001 atcgccgccc cgccgataac ccaggtactg taaacacggg tgatcgccat cacgccaatg
    12061 ttttctccgt aagtggtatt tggcgtggag ccaaagaagc cggagatcac ggtcgacagg
    12121 ccgttagcaa acatcgaacg gtgcagacct ggatcgcgca gcagatcttt tttgacgata
    12181 ttagccgtta ctaccaggtg ccctacgtgt tcggcaataa ccactaacgc cgctggcaga
    12241 atagtcagaa tggcaaacca ctcgaagcgc ggcgtataga gggttggcag cgcaaaccag
    12301 tgagcattaa taatcggcgt ggtatcgaca attcccattg cgaaagagag cgcgtagccc
    12361 accagcacgc caattaaaat cgggataatt gccaggaaac cacgaaacag cacggaacct
    12421 aaaaccgtga ccgccagggt ggtaatagag atgatgatgg ttttggagtc tggcgtttgc
    12481 ccttcagccg ggagtaaacc cgccataccg gcagctacgc ccgccagctc cagaccgatg
    12541 acggcaacga ttgcgcccat tgccgcaggt ggaaacagca cgtccagcca gccggtcccc
    12601 gctttcttca cgataaaaga aaccaggcag aacagcacgc cgcacataat aaagccgccc
    12661 agcgcgactt cataccctaa cggcaacagt aacaataccg gtgaaataaa ggcaaagctg
    12721 gaaccaagat aagccggaat tttcccttta cagatgaaga gatacagcag cgttccaata
    12781 ccgttaaata acagtacagt cgccgggtta atatgaaata agacgggcac caggacggtt
    12841 gcaccaaaca tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa aagtggcggt
    12901 ctttcactca ccccgatagc acggcgcgtc atagtattat cctctgtatt atgtgttata
    12961 ggcgctttac tcaaaaaaaa gccgactctt aaagtcggct ttaattattt ttattcttta
    13021 tttcgtacca aagattttgt caccggcatc gccgaggccc ggaataatgt atccgtgctc
    13081 gttcagtccc tgatcaatcg atgcggtata cagttcgacg tccgggtgcg ctttttccag
    13141 cgcagcgata ccttctggcg cagcgaccag caccagaact ttgatgctgc tgcagcccgc
    13201 ttttttcagc aggtcgatgg tcgcgataac ggaaccaccg gttgccagca ttgggtcaac
    13261 gatcagcgcc atacgctcat cgatgttaga aaccagtttc tggaagtacg gtaccggctc
    13321 cagcgtttct tcattacggt acataccgac aacgctgatg cgcgcgctcg gaacattttc
    13381 cagcacaccg tccatcatac caagacccgc acgcagaatt ggcacaacgg taattttctt
    13441 acctttgatc tggtcgattt ctaccgggcc gttccagcct tcgatagtta ctttttccgt
    13501 ttcgaggtcg gcggtcgctt cgtaagtcag caggctaccc acttcggaag cgagttcgcg
    13561 aaagcgcttg gtgctgatat cttgctcacg catcagtccc agcttgtgtt tgacgagtgg
    13621 gtgtttgact tccacgatct tcatactctt tctcctttga ggggcagcca caaaaaaatc
    13681 gacggattat acctcctttc ttcaaggcgg caatattctt ttcgttgact ttagtcaaaa
    13741 tgataacgat ttgagataaa gttattttat attcaaatgg ttatgaaaga agatcattcc
    13801 atccgaaaac taacctttgc cctggcacaa gtcttctttc gccgcgcgtc tggggaaaag
    13861 acgtgcaaaa aggttgtgca aagcagcctc gcaaacgttt gctttccctg ttagaattgc
    13921 gccgaatttt atttttctac cgcaagtaac gcgtggggac ccaagcagtg accgataaaa
    13981 cctctcttag ctacaaagat gccggtgttg atattgacgc gggtaatgct ctggttggaa
    14041 gaatcaaagg cgtagtgaag aaaacgcgtc gtccggaagt gatgggcggt ctgggcggct
    14101 tcggtgcgct gtgtgcattg ccgcaaaaat atcgtgaacc cgtgctggtt tccggcactg
    14161 acggcgtagg taccaagctg cgtctggcaa tggacttaaa acgtcacgac accattggta
    14221 ttgatctggt cgccatgtgc gttaatgacc tggtggtgca aggtgcagaa ccgctgtttt
    14281 tcctcgacta ttacgcaacc ggaaaactgg atgttgatac cgcttcagcg gtgatcagcg
    14341 gcatcgcgga aggttgtctg caatcgggct gttcactggt gggtggcgaa acggcagaaa
    14401 tgccggggat gtatcacggt gaagattacg atgtcgcggg tttctgcgtg ggcgtggtag
    14461 aaaaatcaga aatcatcgac ggctctaaag tcagcgacgg cgatgttctg attgcactcg
    14521 gttccagcgg tccgcactcg aacggctatt cgctggtgcg caaaattctt gaagtcagcg
    14581 gttgtgatcc gcaaaccacc gaacttgatg gtaagccatt agccgatcat ctgctggcac
    14641 cgacccgcat ttacgtgaag tcagtgctgg agttgattga aaaggtcgat gtgcatgcca
    14701 ttgcgcacct gaccggcggc ggcttctggg aaaacattcc acgcgtattg ccagataata
    14761 ctcaggcagt gattgatgaa tcttcctggc agtggccgga agtgttcaac tggctgcaaa
    14821 cggcaggtaa cgttgagcgc catgaaatgt atcgcacctt caactgcggc gtcgggatga
    14881 ttattgccct gcctgctccg gaagtggaca aagccctcgc cctgctcaat gccaacggtg
    14941 aaaacgcgtg gaaaatcggt atcatcaaag cctctgattc cgaacaacgc gtggttatcg
    15001 aataatgaat attgtggtgc ttatttccgg taacggaagt aatttacagg caattattga
    15061 cgcctgtaaa accaacaaaa ttaaaggcac cgtacgggca gttttcagca ataaggccga
    15121 cgcgttcggc cttgaacgcg cccgccaggc gggtattgca acgcatacgc tcatcgccag
    15181 cgcgtttgac agtcgtgaag cctatgaccg ggagttgatt catgaaatcg acatgtacgc
    15241 acccgatgtg gtcgtgctgg ctggttttat gcgcattctc agcccggcgt ttgtctccca
    15301 ctatgccggg cgtttgctga acattcaccc ttctctgctg ccgaaatatc ccggattaca
    15361 cacccatcgt caggcgctgg aaaatggcga tgaagagcac ggtacatcgg tgcatttcgt
    15421 caccgatgaa ctggacggtg gcccggttat tttacaggcg aaagtcccgg tatttgctgg
    15481 tgatacggaa gatgacgtca ccgcccgcgt gcaaacccag gaacacgcta tttatccact
    15541 ggtgattagc tggtttgccg atggtcgtct gaaaatacac gaaaacgccg cgtggctgga
    15601 tggtcaacgt ctgccgccgc agggctacgc tgccgacgag taatgccccc gtagttaaag
    15661 cgccagctct gccgctggcg tttttcaatt cacctgtaaa tcgcaagctc cagcagtttt
    15721 tttccccctt ttctggcata gttggacatc tgccaatatt gctcgccata atatccaggc
    15781 agtgtcccgt gaataaaacg gagtaaaagt ggtaatgggt caggaaaagc tatacatcga
    15841 aaaagagctc agttggttat cgttcaatga acgcgtgctt caggaagcgg cggacaaatc
    15901 taacccgctg attgaaagga tgcgtttcct ggggatctat tccaataacc ttgatgagtt
    15961 ctataaagtc cgcttcgctg aactgaagcg acgcatcatt attagcgaag aacaaggctc
    16021 caactctcat tcccgccatt tactgggcaa aattcagtcc cgggtgctga aagccgatca
    16081 ggaattcgac ggcctctaca acgagctatt gctggagatg gcgcgcaacc agatcttcct
    16141 gattaatgaa cgccagctct ccgtcaatca acaaaactgg cttcgtcatt attttaagca
    16201 gtatctgcgt cagcacatta cgccgatttt aatcaatcct gacactgact tagtgcagtt
    16261 cctgaaggat gattacacct atctggcggt ggaaattatc cgtggagaca ccatccgtta
    16321 cgcgctgctg gagatcccat cagataaagt gccgcgcttt gtgaatttac cgccagaagc
    16381 gccgcgtcga cgcaagccga tgattcttct ggataacatt ctgcgttact gccttgatga
    16441 tattttcaaa ggcttctttg attatgacgc gctgaatgcc tattcaatga agatgacccg
    16501 cgatgccgaa tacgatttag tgcatgagat ggaagccagc ctgatggagt tgatgtcttc
    16561 cagtctcaag cagcgtttaa ctgctgagcc ggtgcgtttt gtttatcagc gcgatatgcc
    16621 caatgcgctg gttgaagtgt tacgcgaaaa actgactatt tcccgctacg actccatcgt
    16681 ccccggcggt cgttatcata attttaaaga ctttattaat ttccccaatg tcggcaaagc
    16741 caatctggtg aacaaaccac tgccgcgttt acgccacatt tggtttgata aagcccagtt
    16801 ccgcaatggt tttgatgcca ttcgcgaacg cgatgtgttg ctctattatc cttatcacac
    16861 ctttgagcat gtgctggaac tgctgcgtca ggcttcgttc gacccgagcg tactggcgat
    16921 taaaattaac atttaccgcg tggcgaaaga ttcacgcatc atcgactcga tgatccacgc
    16981 cgcacataac ggtaagaaag tcactgtggt ggttgagtta caggcgcgtt tcgacgaaga
    17041 agccaacatt cactgggcga agcgcctgac cgaagcaggc gtgcacgtta tcttctctgc
    17101 gccggggctg aaaattcacg ccaaactgtt cctgatttca cgtaaagaaa acggtgaagt
    17161 ggtccgttac gcacacatcg ggaccgggaa ctttaacgaa aaaaccgcgc gtctttatac
    17221 tgactattcg ttgctgaccg cagatgcgcg tatcaccaac gaagtacggc gggtatttaa
    17281 ctttattgaa aacccatacc gcccagtgac atttgattat ttaatggtgt caccgcaaaa
    17341 ctctcgccgt ctgttatatg aaatggtaga ccgcgaaatc gccaacgcgc agcaagggct
    17401 gcccagtggt atcaccctga agctaaataa ccttgtcgat aaaggcctgg ttgatcgtct
    17461 gtatgcggcc tccagctccg gcgtaccggt taatctgctg gttcgcggaa tgtgttcgct
    17521 gatccccaat ccggaaggca ttagcgacaa cattcgtgcc atcagtattg ttgaccgtta
    17581 ccttgaacat gaccgggttt atatttttga aaatggcggc gataaaaagg tctacctttc
    17641 ttccgccgac tggatgacgc gcaatattga ttatcgtatt gaagtggcga caccgctgct
    17701 cgatccgcac ctgaagcagc gggtgctgga catcatcgac atattgttca gcgatacggt
    17761 caaagcacgt tatatcgata aagaactcag taatcgctac gttccccgcg gcaatcgccg
    17821 caaagtacgg gcgcagttgg cgatttacga ctacatcaaa tcactcgaac aacctgaata
    17881 accctatgcc aatacacgat aaatcccctc gtccgcagga gtttgctgcg gtcgatcttg
    17941 gttcaaacag ttttcacatg gtcatagccc gtgtggtaga tggtgccatg cagattattg
    18001 gccgcctgaa acagcgagtg catctggcgg acggcctggg gccagataat atgttgagtg
    18061 aagaggcaat gacgcgcggt ttgaactgtc tgtcgctgtt tgccgaacgg ctacaagggt
    18121 tttctcctgc cagcgtctgt atagtcggca cccatacgct gcgtcaggcg ctgaacgcca
    18181 ctgactttct gaaacgcgtg gaaaaggtca ttccctaccc gattgaaatt atttccggta
    18241 atgaagaagc ccgtctgatt tttatgggcg tggaacatac ccaaccggag aaaggtcgca
    18301 aactggttat tgatattggc ggcggatcta cggaactggt gattggtgaa aatttcgaac
    18361 cgattctcgt tgaaagccgc cggatgggtt gtgtcagctt tgcccagctt tacttccctg
    18421 gcggggtcat caataaagag aattttcagc gcgctcgtat ggcggcagca caaaaactgg
    18481 aaactttaac ctggcaattc cgtattcagg gctggaacgt ggcaatgggc gcttccggta
    18541 ccataaaagc cgcccatgaa gtgttaatgg aaatgggcga gaaagacggg ataattaccc
    18601 cggaacgtct ggaaaaactg gtaaaagaag ttttacgtca ccgtaatttc gcatcgctga
    18661 gtttaccggg tctttccgaa gagcggaaaa cagtcttcgt tcctggactg gcgattttat
    18721 gcggtgtgtt tgatgcttta gccatccgtg aactgcgcct ttctgacggg gcgttacgcg
    18781 aaggcgtact gtacgaaatg gaaggacgtt tccgtcatca ggatgtgcgt agtcgcaccg
    18841 ccagcagcct cgccaaccag tatcacatcg acagcgaaca ggcccgacgg gtgctggata
    18901 ccactatgca aatgtacgaa cagtggcggg aacagcaacc gaagctggcg catccgcaac
    18961 tggaggcgct actgcgatgg gccgccatgc tgcatgaggt cgggttgaat atcaaccaca
    19021 gcggtttgca tcgccactcc gcttatattc tgcaaaacag tgacttgccg ggttttaatc
    19081 aggaacagca gctgatgatg gcgacactgg tgcgctatca ccgcaaagcg attaaactcg
    19141 acgatctgcc gcgctttacc ttgtttaaga agaaacagtt cctgccactg attcagctat
    19201 tgcgccttgg cgtattactc aacaatcaac gtcaggcaac caccacaccg ccaacattga
    19261 cactgattac tgatgacagt cactggacac tgcgtttccc gcatgactgg tttagtcaga
    19321 atgcgttggt actgcttgat ctggaaaagg agcaagaata ctgggaaggc gtggctggct
    19381 ggcggttgaa aattgaagaa gaaagtacac ctgaaatcgc cgcttaatgc tttgcgggcc
    19441 gacgagaaat gtcggcccgc attattcagg cactttcgcg aatgggttc
//
LOCUS       DAJTSL010000070        19371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTSL010000070 DAJTSL010000000
VERSION     DAJTSL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.070"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="OO433_002205"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="OO433_002205"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6050671.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="OO433_002206"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="OO433_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6050672.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQESNTGILISNGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="OO433_002207"
     CDS             complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="OO433_002207"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6050673.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="OO433_002208"
     CDS             complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="OO433_002208"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6050674.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="OO433_002209"
     CDS             complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="OO433_002209"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6050675.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHLYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="OO433_002210"
     CDS             complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="OO433_002210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6050676.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="OO433_002211"
     CDS             complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="OO433_002211"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6050677.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="OO433_002212"
     CDS             complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="OO433_002212"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6050678.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="OO433_002213"
     CDS             complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="OO433_002213"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6050679.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="OO433_002214"
     CDS             complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="OO433_002214"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6050680.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="OO433_002215"
     CDS             complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="OO433_002215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6050681.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="OO433_002216"
     CDS             complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="OO433_002216"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6050682.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="OO433_002217"
     CDS             complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="OO433_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6050683.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="OO433_002218"
     CDS             complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="OO433_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6050684.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="OO433_002219"
     CDS             complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="OO433_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6050685.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQHPIMRTIKP"
     gene            15346..16338
                     /gene="asnA"
                     /locus_tag="OO433_002220"
     CDS             15346..16338
                     /gene="asnA"
                     /locus_tag="OO433_002220"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6050686.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYLDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="OO433_002221"
     CDS             complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="OO433_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6050687.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="OO433_002222"
     CDS             complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="OO433_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6050688.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARALEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4627 a         5119 c         4854 g         4771 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaagtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca actgaccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc aacagttcgt tgttcggtgc
      301 aacgacgata accggcatat ccgcgtcaat cagcgccagc gggccgtgtt tcagctcgcc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccagtaagg gtgtttttga tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtaattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcagc agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaggccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt tttagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccataa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatgca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccagcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgacca gctttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa caaagttacc gacgtgtgca ccttccagca actcagcacc aggacgcaga
     2401 cgggcaaacg ggccaatagt acaggccgct gccagattca cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttaga gatcagaatg
     2881 ccggtgttgc tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaggtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaaaa ccagcctgtt tccagactgg
     3721 cttttgtgct tttcaagccg gtgttacatc gcttttttgg tcaactcgat aacgcgcagc
     3781 tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt aatctacgtc gccgtgagag
     3841 ctgctaatgt gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc
     3901 tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc cagactgcac ttcaagaatg
     3961 ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca
     4021 ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga tccccagttc accttcgcta
     4081 cccgttacct ggattttctc gaccagacca gagaacattt gttgctctgc gctgacgacg
     4141 tccaggtggt aagtcattgc catatcaccc tccgattaag gcgttaaagt tttttggctt
     4201 tttccacagc ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt
     4261 attcgccttc catgatgcct ttaaagccac ggatggtgtc tttcagggag acgtatttac
     4321 ccggagaacc ggtgaatact tctgccacga agaacggctg ggacaggaag cgctggatct
     4381 tacgagcacg cgctaccacc agtttgtctt cttcagacag ttcatccata cccaggatgg
     4441 cgatgatgtc tttcagttcc tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt
     4501 cgtagtgttc ctgaccaacc accagcgggt ccagctgacg gctggtggag tccagcgggt
     4561 caacggccgg gtagataccc agagacgcga tctgacggct cagtaccacg gttgcgtcaa
     4621 ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc atccgcaggt acgtataccg
     4681 cctgtacgga ggtgatagaa ccagttttgg tggaggtgat acgttcctgc agaacgccca
     4741 tctcttccgc cagggtcggc tgataaccta ccgctgaagg catacggccc agcagtgcgg
     4801 atacttccgt accggccagg gtgtaacgat agatgttgtc aacgaacagc agaacgtcac
     4861 gaccttcgtc acggaatttc tcagccatgg tcagaccggt cagagcaacg cgcagacggt
     4921 ttcccggcgg ctcgttcatc tggccataca ccagggatac tttgtcgata acgttggagt
     4981 cggtcatttc gtggtagaag tcgttaccct cacgagtacg ttcacctacg cccgcaaaca
     5041 cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag ctccatcatg tttacggttt
     5101 tacctacacc cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac ggacacatca
     5161 ggtcgataac tttgataccg gtttccagca gttcctgaga gtttgacagc tcttcgtagg
     5221 aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc gatctcgcct ttcatgtcga
     5281 ccggttcacc cagtacgttc atgatacggc ccagagtcgc tttacctacc gggacttcga
     5341 tcgggtgttc gaggtctttt acatccagac cgcgacgcag accgtcggag gaacccattg
     5401 cgatggtacg cacgataccg ccgccgagct gctgctgaac ttccagcacc agacgctcat
     5461 taccattttg cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga gggaattcga
     5521 cgtcaactac ggcgccgatt acctggacaa tctttccagt agccatctta aatcctctac
     5581 gaaataacct gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt cctgagtaat
     5641 gctggcctga cgagctttgt tgtataccaa ctgcagctct ttaatcaggc tgccgccatt
     5701 gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc tcgctggcca ggttttcaac
     5761 cacgccctga taaacctgag attcgacata acgacgcagc agggtatcca gcaacgcctt
     5821 cggatcgggt tcgtacaggt aatcccagga tttatgtttc agatcatcat catctgatgc
     5881 cggtaacggc agcagctggc tgatggtcgg aacctgagac atggtgttaa taaatttgtt
     5941 gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag gcctgcaaca tcacttttac
     6001 cggaccgatc agttcggaca gggaagggtt atcccccatg ccggtgacct gggcaacaac
     6061 attgccgccc acggagttga agaacgacac gcctttcgag ccgatcattg cgaggtcgca
     6121 ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc agttttttga acaggttaat
     6181 gttcaaacca ccgcacaaac cacggtcggt cgacaccacc aggtagccca cgcgtttaac
     6241 gtcgcggtct tccaggtaaa ggtgcttata ttccagatta ccatgtgcaa ggtgaccaat
     6301 cactttgcgc atggtttctg cataaggacg gctggccgcc atgcgatcct gcgatttacg
     6361 cattttggaa gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt tctggacgct
     6421 tgcgatctta ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg
     6481 cctgccctaa ggcaagccgc cagacgttac caggattggg ttgctttgaa ggaatcgagg
     6541 atgcctttca gtttgccttc gatttcgtcg ttgtagccac cggtctggtt gatctcttgc
     6601 atcaacggag cgtgatcacg gtcgacgtaa gccagcagag cggcttcgaa gctgccaatt
     6661 ttcgacagtt caacatccgc caggtaacca cgttctgctg cgaacagaac cagagactgc
     6721 tgcgcaacgg acatcggcgc atactgtttc tgtttcagca gttcggtcac tttctgaccg
     6781 tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg caaactgaga gaacgctgcc
     6841 agttcacgat actgtgccag agcggtacgg ataccaccgg acagtttttt catgatcttg
     6901 gtctgtgctg caccaccaac acgggatacg gaaatacccg ggttaaccgc aggacgaata
     6961 ccggcgttga acaggttggt ttccaggaag atctgaccat cggtaatgga gattacgttg
     7021 gtcggaacga acgcagaaac gtcacccgcc tgagtttcga taatcggcag tgcggtcaga
     7081 gaaccggttt tccctttcac ttcacctttg gtgaaggctt caacgtattc ggcgttaaca
     7141 cgtgcagcac gctccagcag acgagagtgg aggtagaaaa cgtcgcccgg gaatgcttca
     7201 cgtcctggcg gacgacggag cagcagggag atctgacggt aagcaacagc ctgtttagac
     7261 aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac ggaagtattc gcccattgcg
     7321 caaccggcat acggtgccag gtattgcagt gcagcggatt cagacgcggt tgctaccaca
     7381 acgatggtgt tagccagtgc gccgtgctct tccagtttac gtaccacgtt agaaatggtg
     7441 gacgctttct ggccgatagc gacatagata catttgatac cggaatcgcg ctggttgatg
     7501 atggcatcga tagccagtgc ggttttacct gtctgacggt caccgatgat caattcacgc
     7561 tgaccacgac cgattgggat catggagtca acggctttat aaccggtctg taccggctga
     7621 tctacggact gacgttcgat aacgcccgga gcgattgctt ctacagcaga gaagccgtcg
     7681 tgatccagcg gacctttacc gtcgattggt gcacccagag tgttaaccac acggcccagc
     7741 aggccacggc caaccggaac ttccaggata cggccagtac acttaacttt catgccttcg
     7801 gcaaggtcag cgtacggacc cataacaacc gcacctacag agtcgcgctc gaggttcagt
     7861 gcgatagcgt aacggttacc cggcagggag atcatttcac cctgcataca atcggccagg
     7921 ccgtgaatgc ggataacacc gtcacttaca gaaacaatag taccttcgtt gtgagcttca
     7981 ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc
     8041 agttgcatgc tccagtcccc ttaagactgc aagacgtctg caaggcgctc aagacgaccg
     8101 cgtacgctgc catcaatgac catatcaccc gctcggatga taacgcctgc cattacagac
     8161 ttatcgattt tgcaattcag cttaactttg cgtgacagac gtttttccat cgcagcagaa
     8221 attttcgcga gctgttgttc actcagtgcg gcagcggaaa tgacgtctac ctcagcggta
     8281 gcctcactca cggcacgaag gtgaataaac tgctccagaa catccgggag cgcgttaaga
     8341 cgaccatttt cagccattac ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca
     8401 caaactgcga taaacgactc ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct
     8461 gccatttgtt cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct
     8521 acactttggt gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta
     8581 ataaattcag acatcagccc ctccctcctt acagttcagc gacaagttta tccacgatgt
     8641 cgctgttagc agcttcatcc acggaacgtt cgatgatctt ctcggcgcca gcaacagcca
     8701 ggatagcaac ttgcttacgc agctcttcac gggcacgttt acgctcggct tcaatttctg
     8761 cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc ctcagctttc gcttcgtcca
     8821 gaatctgcga gcggcgtttg ttcgcctgct cgatgattac ctgggcttcc gctttcgctt
     8881 ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt gctcgttctg
     8941 cggaagcaag gccgtcagca atttcttttt gacgtttttc gatggctgcc attaatggcg
     9001 gccatacgta cttcatgcag aacagaacga acaggacaaa cgcgatggcc tggccgagga
     9061 ttgttgcgtt aagattcaca gcacaatgcc tctatttagt taacgttctg atattgctct
     9121 ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca cgtacagacc cagacctaca
     9181 gcgatcatcg ggatagcatc caccagaccc ataacgataa agaactgagt acgcagcaga
     9241 ggaatcagat caggttgacg cgctgcgcct tccaagaatt tacccccgag gatgccgata
     9301 ccgatcgcag caccgattgc cgccagaccc atcatcacag cggcagccat gtacagcaga
     9361 tccatattca ggttttccat gacagtctcc agtttgtttc agttaaaacg tagtagtgtt
     9421 ggtaaattaa tgttcttcag acgccatcga cagatagacg atcgtcagaa ccatgaagat
     9481 gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc cacggcacat tcaggatcca
     9541 ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa atcagctcac cggcatacat
     9601 gttaccgaac agtcgcaaac cgagtgaaac tggtttggac agcaggctta ccccttcaag
     9661 gattaagttg acaggaatga acgcccagtg attgaacggc tgcagcgtca actctttcgt
     9721 gaagccgccg atgcctttca ttttgatgct gtagaacaga atcaggataa atacgcccaa
     9781 tgccatagac agcgttacgt tcacgtccgc agacggaacc acacgcagtg caggcagacc
     9841 cagtacatgt tcagcaatgt acggcagcag gtcgataggc agtaaatcca tcaggttcat
     9901 caggaatacc cagacgaaga tcgtcagggc cagcggagca atcagcttgc ttttgccatg
     9961 gtacatgtct ttcacgctac cattaacaaa gccgatcacc agctcaatcg cggtctgaaa
    10021 cttacctggc acaccgctgg tcgccttttt ggctacgcta cggaataaaa ccaggaacaa
    10081 cagacccagc accaccgaga agaacatgga gtcaatattg attgtccaga aggtggctgg
    10141 ggggttttgt ggatccacca gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg
    10201 gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg
    10261 ttgttaatta cagccggtgc cagtatctga accaccagca ccaaaaccca cgtaacgatc
    10321 agcggcaaga ataccgcctt taaaaccgcc aacgccacca ccagtaacac caacatcgcc
    10381 agaactttga aagcttcgcc aaatgcgaat gtccaggcca cccggccttt cgctggtgta
    10441 tgcgcctggt gacgccaggc aaatatcata aacaaaacgt taggcagaaa gactgccagg
    10501 cccccgctta ttgcagagac gccccagaag gggtctttga ggctgaacag caatccactt
    10561 gctatcacca ccagtaactg aacgagcaga agcttccgag caacgtttcg actcacgagc
    10621 gacacagaca tcacgttttt tactcctgct cccttcgagg tatgccgcgt gtcgtataaa
    10681 actttcttta aggcttagag tcaagcatca aaaagcggtc aaattatacg gcgcgccccc
    10741 gtgatttcaa acaataagta gccaaaaggt gaataaatgt ttaaatattt ttccagtgca
    10801 tacaattgcg acttttctac taaccctgtt cgatcatgaa aaactgtaaa taacgcgtaa
    10861 acactggtga taaagcgtgc ttcagatcac atattgcgca tgttcgcgca cagcatattt
    10921 atttacttgg caaatgatgc ctttgcaagt ttatgatatt tcagtctaaa aacagatact
    10981 gttttaataa atgacattta cacaacaaaa accacccatt gacattttta ataatgtttt
    11041 agcagccaat gatggttctt agcgccgatt tttagcagac tgatattttc actaatgact
    11101 tattttctgc ttaccaaaaa aagccacgtt atcttgttga tgcaaaagag tgaacgtggc
    11161 gttaaatgta accagttata tcagtagaaa acctggttgt tgttaacagt ctaaccggtc
    11221 aattttttat gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg
    11281 acgttcgcca tccagggctg gaacctgaag tttaaccact gattcgacct gatattcttc
    11341 gggcaacaaa gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg
    11401 ctcaccagga agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct
    11461 aattacgcca tcaaatggcg gctctgaagg aaactcttct accctgctct gtacaggttc
    11521 aatattctcc agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag
    11581 gctatccaac agagtgaaat gggcttcagg acgcacgata gagagtggaa tgcctggcag
    11641 tcctggtccg gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat
    11701 gctatcgaga atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta
    11761 cgctttgttc catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc
    11821 ggtaagcgaa atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac
    11881 ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc
    11941 gctacgacgc agcataccct gttttttcag ccacaccagc agaatggaga tagccgcagg
    12001 cgtgacgcca gaaatacgcg aagcttggcc gatagaggct ggtttgtgat cgttaagttt
    12061 ggcgatcact tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag
    12121 ggtgttctcg ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc
    12181 gtatttaacc tgaatctcaa cctgttccgc cgcctgttcg tctgtcaacg caggggcaaa
    12241 cggcgtcagc gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc
    12301 actggcttca cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc
    12361 cgacggggtt acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt
    12421 ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt
    12481 caaacgcaga tccgcattat cttcgcgtag catcagacga tattctgcgc gcgaagtaaa
    12541 catacgatac ggttctttgg ttcctaaagt gcacaggtca tcaactagta cgccgagata
    12601 cgcctgagaa cgtgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag
    12661 accggccagc aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc
    12721 agcaaagaac agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgaggatc
    12781 gaagaagtca tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg
    12841 catagagcgg acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg
    12901 ataaatttca ttagaggtca gtccttccgg ttcaaggaag atctgatgct gatttctgtc
    12961 ggcgaagcgc atgactttgt cttcgatcga cgggcagtag cgtgggccga caccttcgat
    13021 cacccctgca tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc
    13081 gttggtatga gtgatataac acggcacctg ctggggatgc tgggacgcat tgcccataaa
    13141 cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc agcacactaa agtcgatagt
    13201 tcgagcatca atacgcggtg gtgtcccggt tttcagacga ccaacgcgca gcggcagttc
    13261 acgcaaacgg cgagaaagcg gaatggacgg cggatcacca gcacggccac cgctgtaatt
    13321 atccagaccg atatgaattt taccgtcgag gaacgtccca acggtgagca cgactgcttt
    13381 ggcacggaac ttcagtccca tttgggtaac agcaccgacc acgcgatcgt tttcgacaat
    13441 aagatcttca accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg
    13501 taccgcctga cggtagagca cacgatccgc ctgagctcgg gtagcgcgaa ccgccggtcc
    13561 tttgcttgcg tttagtatcc taaactggat acccgcttga tcgatcgctt tcgccatcag
    13621 accgccgagt gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg
    13681 gttgcagctc atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc
    13741 catacgcgcc gcggccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac
    13801 gtcaaaagga tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat
    13861 tgaagcccgg gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg
    13921 ggtattaaaa aggagatcta tttatttaga gatctgttct attgtgatct cttattagga
    13981 tcgcactgcc ctgtggataa caaggatccg gcttttaaga tcaacaacct ggaaaggatc
    14041 attaactgtg aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta
    14101 tacacaactc aaaaactgaa caaccgttgt tccttggata actaccggtt gaaccaagct
    14161 tcctgacaga gttatccaca gtagaacgca cgatctgtat acttatttga gtaaattaat
    14221 ccacgatccc agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat
    14281 cttcagtgtt tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc
    14341 gatagcccca caaaaggtgt catattcacg actaccaata ccgattgcgc caaagcggac
    14401 cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg
    14461 aatatctccg gcaccgtggg tggagctgat aatcagccag atccccgagg cagataaatc
    14521 ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc
    14581 caggtgttct gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc
    14641 tgccataaac cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat
    14701 ctacctgtgg aaaagtatgg gattaaaaaa gccgatcagg gcttgatagt acgcatgatc
    14761 gggtgttgca ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg
    14821 ttgataagta catgctggag agcgtcgatc gaacggcaca ttacttttat aaagatgctg
    14881 tagtggcctg ttgtgtaata ggcttcagtg acttcatcaa ggctttccag ctttgccagc
    14941 gcggaagggt agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa
    15001 ccgagctgct tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt
    15061 ttctctaccc gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg
    15121 taagcggtgc gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc
    15181 agataatttt ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct
    15241 tttttaatga atcaaaagtg tgttaggctt tttattgaat gattattgca tgtgtgtcgg
    15301 tttttgttgc ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac
    15361 attgccaaac aacgccaaat tagcttcgtg aaatctcact tttctcgtca actggaagaa
    15421 cgtctggggc tgatcgaagt ccaggcaccg attcttagcc gtgtggggga tggcacgcag
    15481 gataacttgt cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc
    15541 cagttcgaag tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac
    15601 ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagaccgt
    15661 ctttctccgt tgcactcggt ctatgtagac cagtgggact gggaacgcgt aatgggcgac
    15721 ggtgagcgtc aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa
    15781 gcaaccgaag ctgcggttag cgaagagttt ggcctggcac cgttcctgcc ggatcagatc
    15841 cacttcgtac acagccagga gttactgtct cgttatctgg atctcgatgc caaagggcgt
    15901 gagcgggcga tagcgaaaga tcttggcgcg gtattccttg tcgggattgg cggcaagctg
    15961 agcgatggtc atcgccacga cgtgcgcgca ccggattatg atgactggag caccccgtca
    16021 gagctgggtc atgcgggtct gaacggcgat attctggtgt ggaaccctgt actggaagat
    16081 gcgtttgagc tttcttccat ggggatccgc gtggatgccg acacgctgaa gcatcagctg
    16141 gcgctgaccg gtgatgaaga tcgtctgcag ctggaatggc atcaggcgct gctgcgcggt
    16201 gaaatgccgc agaccatcgg cggcggtatc ggccagtctc gtttgactat gctgctgctg
    16261 caactgccgc atatcggcca ggttcagtgt ggagtatggc cagctgctgt tcgcgagagc
    16321 gtcccttctc tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc
    16381 cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg
    16441 acatcgccac ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg
    16501 tcacgtcgtc aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa
    16561 accattccct gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc
    16621 cgccacgaaa ctgctggctt aaaaaacgga ttgcttgttc gatgccttgt gggcctgaaa
    16681 gctcataacg gacgatctcg gtggaaaata gcataatata gcagcgccgg ttttctgcga
    16741 gagcaatgcg catcaaggcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca
    16801 ttgagccgga agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt
    16861 tatgtaccac cggacgttcg atcacttttt cacgccacga ctcaccgtgc aggcgatagg
    16921 tgagcaactg tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta
    16981 gtgtcgccag ttctggcggc aggagacgta aaatatcatc gctttgttgc agaccatcaa
    17041 cctgctcagg aaccgtcgcc ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat
    17101 cgttgcgcgg tattgatttg gcttcccgag aacgccccag ctgctctgcc aggcgtttca
    17161 gttccggctg ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt
    17221 taagctggcc ggcgctcata tcccacagac gaccagctgc ggtattgtta tctgcgagaa
    17281 tcggttcaag ttgtccgctc agcgtcatgc gttcctgaac ttcacttaac agttgttcgc
    17341 gttcttcttc taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc
    17401 agcgctggag aaaaagcgtg tgtaacgctg aagttattgt gctgttagca tcaaccaact
    17461 gccgggcttg ttctgcccat ggggaattca gacgatgcag taagtccagg atctgtggta
    17521 gctgcacaat aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt
    17581 cggtgagttc tggcgggact cgggcatctt tcagccgact gcgcagcgcc tcacgccagc
    17641 ggggaacatc atcagtgatt gctgccttca gtcgtgggaa tttttcaaag aagaccgcca
    17701 gctgcggtga ggccagcagt gcgatgatca tctcttcgat caatccctct tcgctgacgg
    17761 ccagcatcac attaagcgta tccagcgtta gcattgttgt gcctggcgaa tctgtgcacc
    17821 gacatcctgt aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt
    17881 ttgctgttcg ctgaataaag cgtgttgctt acgccagtcg ctttcaagct cttccagttg
    17941 ttgttttatt tcacctggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc
    18001 ttgtaaactt acatcgcgta caacaagatg ttgggtgcta tcaacttcca gattcagttt
    18061 ctgggcaaag ccgataccgt tcagtttgcc gcgaatttca ccacctttac tcagccactg
    18121 ttccagcgcg ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg
    18181 tttttgcagc agcagagtca gagtggaagc agtaacgtta acagggagtt gatactgctg
    18241 acgacggcta aaaatgccgc ccagacgaat taccgttaag gctgttttat cgctttgttg
    18301 ctgctgaagt tgcaggtgac gttgcacaat cgcgcctagg cgggtcaaca tcccttgctg
    18361 ttgccaggcg tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg
    18421 cgcgtcatac cacaggcaat ctttcagcaa aatgagatct accggggcaa cagcactgcg
    18481 accgctaaaa aaggcgctgg cctgcaataa tcggatcgct tttttccagc gacgatccga
    18541 gacataaggc gcatccggta atttatccag ttgctggcgc agcataaaaa tcagctcaaa
    18601 tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc
    18661 tgtgacctgc aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat
    18721 ggagcggaaa ttcgctttat cctgcacttt atctaaccac agacgaatca gcatgcggtc
    18781 atataacgct tccagactgc tgtctgcttc cggcagctcg ttggaggccg ccaccagcag
    18841 acgcatcggg attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt
    18901 gagcaaggta ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacaatttc
    18961 tgcttccggc aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg
    19021 aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaag
    19081 cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc
    19141 tggcgggcca aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat
    19201 ggcgtggcta cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc
    19261 cgctaataaa tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta
    19321 aattgcgagt atagacgttt cttgctggtg gctaaaatag tctcaaaggg g
//
LOCUS       DAJTSL010000071        19086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTSL010000071 DAJTSL010000000
VERSION     DAJTSL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.071"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(31..2478)
                     /gene="glgP"
                     /locus_tag="OO433_002223"
     CDS             complement(31..2478)
                     /gene="glgP"
                     /locus_tag="OO433_002223"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6050689.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="OO433_002224"
     CDS             complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="OO433_002224"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6050690.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRASLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="OO433_002225"
     CDS             complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="OO433_002225"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6050691.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLKAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="OO433_002226"
     CDS             complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="OO433_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6050692.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="OO433_002227"
     CDS             complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="OO433_002227"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6050693.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDASISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9672..10775)
                     /gene="asd"
                     /locus_tag="OO433_002228"
     CDS             complement(9672..10775)
                     /gene="asd"
                     /locus_tag="OO433_002228"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6050694.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10967..11560
                     /gene="yhgN"
                     /locus_tag="OO433_002229"
     CDS             10967..11560
                     /gene="yhgN"
                     /locus_tag="OO433_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6050695.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="OO433_002230"
     CDS             complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="OO433_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6050696.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGPLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="OO433_002231"
     CDS             complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="OO433_002231"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6050697.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMLRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="OO433_002232"
     CDS             complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="OO433_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6050698.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVSYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="OO433_002233"
     CDS             complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="OO433_002233"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6050699.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15664..16701)
                     /locus_tag="OO433_002234"
     CDS             complement(15664..16701)
                     /locus_tag="OO433_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6050700.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFISDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLSVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     PEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17033..17521
                     /gene="yhhY"
                     /locus_tag="OO433_002235"
     CDS             17033..17521
                     /gene="yhhY"
                     /locus_tag="OO433_002235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6050701.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRSSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17758..18957
                     /locus_tag="OO433_002236"
     CDS             17758..18957
                     /locus_tag="OO433_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp1 family type VI secretion system effector"
                     /protein_id="HCR6050702.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLSKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPPMKNVMNASH
                     TIKHGAV"
BASE COUNT         4885 a         5050 c         4646 g         4505 t
ORIGIN      
        1 cctttggccc cgttctattt attggtgaac ttacaatctc accggatcga tatgccagat
       61 atgatcggcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
      121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg
      181 acaatcgaca tagctgcgat aatccgccag aacctgatag tgatcgccga agttgatcag
      241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
      301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
      361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc
      421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
      481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg
      541 ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
      601 gaacacgact ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
      661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
      721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
      781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
      841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
      901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
      961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
     1021 gtccagcacg gctgaaagcg atgggttcgc taccgctagc cagcgacgcg gcgtcacacc
     1081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
     1141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
     1201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
     1261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
     1321 aaagatgatc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
     1381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
     1441 cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
     1501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
     1561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccagaaagta
     1621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
     1681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cttggttgaa
     1741 tttaccgagg ttaatttcgc tactggcttg cgcactccac aaacgcaacg tgttggtcgc
     1801 gtcggtgtcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
     1861 aatccagcgc gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg
     1921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
     1981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
     2041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
     2101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
     2161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
     2221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg
     2281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
     2341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
     2401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
     2461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
     2521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
     2581 aaaccgccac agtgaagcac gagaccacag tacaaaagca cgtcgaatag cccgtaacag
     2641 cgaccaggca ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc
     2701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
     2761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
     2821 aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
     2881 gcccacctgg ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
     2941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agacccggta aggcttccag
     3001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
     3061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
     3121 caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt
     3181 ctcgtccacg ccgttcagta cgccggaaag acgcccttca cggtgacgct gttgcaacag
     3241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
     3301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
     3361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
     3421 ataaaacatg ccttgatagg ccaagttgtg cacagtaaac accgacttcg ccggacgccc
     3481 gcgcgccgcc agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc
     3541 aggacgccag aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc
     3601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
     3661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
     3721 caacagcgtg atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtcac
     3781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
     3841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
     3901 catctctgaa catacatgta aaacttgcat tatcgctcct gtttatgccc taacttccgt
     3961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
     4021 tcctctgcgt tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg
     4081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
     4141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
     4201 ccggagatca cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
     4261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
     4321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa
     4381 gctttcagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
     4441 gagagcggga acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct
     4501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
     4561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
     4621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
     4681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
     4741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
     4801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
     4861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
     4921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
     4981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccggagttg
     5041 atgcagttag acagcgcaaa gtcgataatg cggaacttac cgccgaagtg tacggccggt
     5101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
     5161 gcaacagatt tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta
     5221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
     5281 gccagacagc cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
     5341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
     5401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
     5461 gttgagcata tcgatttagc caacggacat tgccatcgcc ttcttcccac cagcgattct
     5521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
     5581 cactgcttgc ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt
     5641 tattgccatg ctggctgtga ccatgttcgt caccggccag taacatcggc gtaccctggg
     5701 agagcaacaa cgttgttaac aaggcgtgaa tactgtcgcg ccgccgttca accaggtcaa
     5761 gagaaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
     5821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
     5881 taaaaccgtc atgcgcagtg acgagattaa tcgcggcact cggcagacga ccattacgtt
     5941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
     6001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
     6061 ggaaatttcc cacctgataa ccaccagggg cgatatccca cggttcagca attaacttca
     6121 cctgcgagag caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
     6181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tgacaggttt
     6241 ctacccaata acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga
     6301 gcgtgttgcc gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
     6361 tacggttatc gatcccacgc agtgagaata acgggccgtc gaggtccagt tccgcactat
     6421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
     6481 cgcgaaactc atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
     6541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
     6601 tggcaaactg cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt
     6661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc ggaatctccg
     6721 ggtgcaagta cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg
     6781 tgcgcggcgg ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg
     6841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
     6901 ctttaaattc cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
     6961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
     7021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc
     7081 gatgttcctg gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga
     7141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
     7201 tggcgagttg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagtgt
     7261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
     7321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
     7381 gcctggctga ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc
     7441 aacgataatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
     7501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
     7561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
     7621 atcgccacct tccaacagat gccagtcgag gctggcgtca tggttccact cacggccctg
     7681 ggcaaattcg ttccccatga acagcagttt cttgcccggg aatgcccaca tccagccata
     7741 gtaggcgcgc aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
     7801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
     7861 gtagagaatc ccgaaggtga gtttatcgtg atgatactga cgataaaccg ggtcgagctt
     7921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
     7981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
     8041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
     8101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
     8161 gcgataaatc attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
     8221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat aattgtagat
     8281 cagcgtattc cagtcctgat gatagccttc gcgcggatcg ctgtgttcat acaagttcgt
     8341 gccatcaaat tcggcaagcg caaagtcatc ggtcgggaag tggcctggca cccagtcgag
     8401 aatcacgttc agacccgctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
     8461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
     8521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
     8581 caccagttga tcggccagct cgcggtagct caaccagaaa ttgttatcgg tgtggcgacg
     8641 ccaggagccc aggtgaactt cataaataga gattgacgca tcaaactgat tcgctttttt
     8701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
     8761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
     8821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccaaat
     8881 gccgctctct ttacgcagac gcatcgggtg acggcgaccg tcccagtagt tgaattgccc
     8941 aaccaccgag acccgacggg cgtttggagc ccaaacagag aaacgcgtac ctgtgacgcc
     9001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
     9061 taatagccag gcatccattt cctggattag cgggccaaaa cggtaaggat catcaatcag
     9121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
     9181 aatgacaccg ctaaagaatc cccgtgagtc aagacactcc agttttgcga gtttgcgccc
     9241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
     9301 tcccgcggtg gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat
     9361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
     9421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
     9481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
     9541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
     9601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
     9661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
     9721 agcagctggt cacccacggt aaaggctgac aggaactccg gtcccatatt cagcttacgc
     9781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
     9841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
     9901 tcgacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
     9961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    10021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt
    10081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    10141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    10201 gtcgcgagtt catctgccac atggccatac agatggccca tctgggttaa taactcacgc
    10261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    10321 agatcattgg cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg
    10381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    10441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    10501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    10561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    10621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    10681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    10741 ataccgcgcc agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg
    10801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    10861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    10921 tgttagcaca cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat
    10981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat
    11041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    11101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    11161 cagcctgcga gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat
    11221 taagatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    11281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    11341 gttgttgtct catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc
    11401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    11461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca
    11521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa
    11581 gaacccggta gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat
    11641 catcccaacg atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag
    11701 cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg cgtcgttaac
    11761 gtggctgaca acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc
    11821 ggagtagttc agttgttcaa tgaccggcat caccagcccc accgccgtta aacaggcaac
    11881 ggtggcagac ccctgaatga tgcgcactgc agcggccagc acgaagcagg tgatagcaat
    11941 cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag
    12001 cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgtag
    12061 cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc
    12121 ataaatcgct accagacaag caaccagaat cgcggtaaat ggatgaccaa taaactcgaa
    12181 ccattcgtaa gcagttgatc cttctggcac aaaacgagcg gcaatggttt tcagccccac
    12241 cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaggacggca ttttgccttc
    12301 gccgagatgc ggttcgctga tgtcgtcagg aatatgcaac tccacgtaac ggctgatgaa
    12361 attaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca gaccaatcag
    12421 gatcatccag ccaaagtcgg cattcatctg cgatgccagc aacatcggcg ctggtccagg
    12481 caccagaaat gccgccgctg ccgccacgcc tgcgaataat gggattacca gcttcaccag
    12541 gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa ctatcgccac
    12601 ttcaaagaac agcggtagcg cacagaccaa ccccgcaagg ccgatggcat aatgcgcgcg
    12661 gctgtgaccg aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcatg
    12721 taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggggcc
    12781 tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc
    12841 agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa
    12901 aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac
    12961 ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc
    13021 gatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg
    13081 cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaagc gactttcaat
    13141 cacatcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa
    13201 gtcgcgatag tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgcag
    13261 catagcaaac gcggcgtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag
    13321 cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat caccatcaag
    13381 aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga
    13441 tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc
    13501 acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac
    13561 aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt
    13621 gtaggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt
    13681 cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc
    13741 acgcggcgtc agcacgctcg ctaaccgcgg ctccatcacc tggccaatgt catgaccgtg
    13801 gaaaccggca atcgccatat cgtcaggaac cttcaatccc agacgctgac attcaaacgc
    13861 cgcgccgacc gccaggtcat cgttggtaca gaacacgcca tccagctgcg gatattcccg
    13921 ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac
    13981 gctatacgac accagacctg catccagcat cgcctgttcg tatcccttct gtttgatgat
    14041 agtacgttcg tcgagacgcg cgccgagata ggcaatgtgg caatgcccgc gagcaataat
    14101 ggcggtggtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca
    14161 cggcgactgg ctgtccatga gttccaccac cgggatcccc gccacttcaa tcatctttaa
    14221 ggtgcgcggc gtgtgggtac gttcggtgag gatcagtccg tcgatattcc atgagagcat
    14281 ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgagcca gcatggtctg
    14341 ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt
    14401 ggtgagagaa ggtaacagga cgccaatcgc ccggctggtg gcgttagaga ggatatcggg
    14461 cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc
    14521 gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac
    14581 acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa
    14641 gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct
    14701 tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc
    14761 aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg
    14821 caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc
    14881 tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg
    14941 tcgaggcttt cacgccgtta atggtgacat tgcctttcac cacctggatc cagacgcggc
    15001 gttcagcggc aatctgatgc accgactgct catctttcag caacgcccag cggtacagtt
    15061 ccatatcctg atgcactttc aatgaaccat ctcgcgcatc cggcgagagc accagctgtt
    15121 tgccctgtac ggcatcgaag cgacgctgtt cataacgcgg cgtaataccg ttttcttcag
    15181 gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt
    15241 gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat
    15301 tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat
    15361 ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc
    15421 gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc
    15481 aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca
    15541 tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat
    15601 gatagagggt gaaaattgcc ccctctgttc aaaaaaattg aactattcga ggggccatca
    15661 aacttaattc gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat
    15721 ttccagattg gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt
    15781 ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct
    15841 gaccgtcacg ccctcgtcat tgacatactc cggcacaccg accgaatcat ccgctgcgaa
    15901 tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta
    15961 tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag
    16021 atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc
    16081 gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc
    16141 gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccataaaacg cgccatcctg
    16201 cggcagccca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc
    16261 aacaatctct cccaacttgc cactttcaat cgcttttttc gctgtcagga agcaggagtc
    16321 aaagcgacga ttctgatacg gcgtgacgct cagccctttg cttttcgcca acgcaaacag
    16381 ctccttcgcc tgggcaagcg tcggggtgaa cggtttttcg accagcacat ttttcccggc
    16441 ttccagcgcg cgtttcgcgt actcgaaatg gctgtccgcg tgggtgcaga caacaaccag
    16501 cttaacatcg ggatcgttta gtacttcgtc gagatcgctg atgaaatgga tatgggaata
    16561 aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct
    16621 atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat
    16681 aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg
    16741 caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa
    16801 attgagaacg aaagatcaaa aaaaagccag cacccggctg gctaagtaat actggaagca
    16861 atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg cgagacaata
    16921 ataatcattc tcattcgcgc ttgtccaaca ctttttgcaa aaaaatgcat ttgactcgca
    16981 tttgaaagtc aatgatgttg aaagggacat ttaccccaaa gaggacaaag gaatgagtga
    17041 gatagtaata cgccacgcag aaacacggga ttacgaggcc atcaggcaga ttcacgccca
    17101 gccggaggtg tattgcaaca cactacaggt gcctcatcct tccgatcata tgtggcagga
    17161 gcgactcgcc gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg gagacgtcgt
    17221 gggccatctc accattgacg tgcaacagcg cccacgcagc agtcatgttg ccgattttgg
    17281 tatctgtgtc gactctcgtt ggaagaaccg cggcgtcgcc agcgccctga tgcgagagat
    17341 gattgaaatg tgcgacaact ggttgcgggt agatcgcatt gaactaaccg tgtttgtcga
    17401 taacgccccg gcaattaagg tctataaaaa atacggcttt gaaattgaag ggactggtaa
    17461 gaagtacgca ttgcgtaatg gtgaatatgt cgatgcatat tatatggcgc gggtgaagta
    17521 agatagtgcc ctttttctga gatggaaaaa gggtgtcatt caaaatcgac ataccttcct
    17581 ttaaggtatt tatttaccca atacatatat tgagatttaa ctcataatta attcctaaag
    17641 tgtaatattt tattttttaa tatatacgcc tacaatttcc tggagtaaat aaataacaat
    17701 taacaagcat aatattgcca ttgataaaat agcatgccat aaaaggactt ttcagggatg
    17761 agtaatattg tttacctgac agtaacggga gaacaacaag gaagcatctc cgcaggttgt
    17821 gggacttctg agtctacagg taatcgttgg cagagcgggc atgaggatga aatatttaca
    17881 ttctcactct taaataatat taataatacg gggcttggtt cacagttcca tggtataaca
    17941 ttttgtaaat taattgataa aagcactcca ttatttatta attccattaa caataatgaa
    18001 caattattta tgggatttga cttctatcga ataaatagat ttggtagatt ggaaaagtat
    18061 tattatatac aactaagagg cgctttttta tcggctattc atcaccagat cattgaaaac
    18121 caactggata cagaaacaat aactattagt tatgaattta tcctctgtca acatcttatc
    18181 gcaaataccg agttcagcta tttggcactc cctgaaaatt ataaccgatt gtttttacca
    18241 aattcaaaaa accaaacaaa taatcgtttc aaaacgttaa acagcaaagc tattggcagg
    18301 ctacttgctg ctggtggcgt atacaatggg aacattgaag gattcagaga tactgcggaa
    18361 aaactgggtg gagatgcaat aaaaggctat gatcaaatac taaatgaaaa aacagcgggc
    18421 atagcgatag caacagcatc tattctttta acaaagcgtt ctaatgttga tacatataca
    18481 gaaataaata gttacttagg caaacttaga ggtcaacaaa aacttcttga tggtatagac
    18541 ataatagaaa taatatacat taagagacct tcaaaagact tagctaactt acgaaaggag
    18601 tttaataaaa ctgtaagaaa aaattttctt atcaaacttt caaaaacctc cgaagcatct
    18661 ggaagattca acgccgaaga ccttttaaga atgagaaagg gcaatgttcc tctaaattat
    18721 aatgttcacc ataaactatc tctagatgat ggtggtacta atgatttcga aaatttagta
    18781 ttaatcgaaa acgaaccata tcataaagtt tttactaaca tgcaatcacg aatagctaag
    18841 ggaatattag taggtgaaag caaaatcact ccctgggcca ttccatctgg ctcaatttat
    18901 cctcccatga aaaatgtaat gaacgcatcc cacacaatta aacacggagc agtatgatgg
    18961 ttaaaagggt gaaactggca ggtgtggagc ctcgcagatt tccggcatca tagtgcccaa
    19021 tacgggatgc ataaagctcc ctcagactgg aaactccaca aatgaaatat acaccggttg
    19081 gcgttg
//
LOCUS       DAJTSL010000072        18983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTSL010000072 DAJTSL010000000
VERSION     DAJTSL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.072"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..739)
                     /locus_tag="OO433_002237"
     CDS             complement(<1..739)
                     /locus_tag="OO433_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR6050703.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMT"
     gene            1206..2438
                     /gene="mdtM"
                     /locus_tag="OO433_002238"
     CDS             1206..2438
                     /gene="mdtM"
                     /locus_tag="OO433_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCR6050704.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            2479..3759
                     /locus_tag="OO433_002239"
     CDS             2479..3759
                     /locus_tag="OO433_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCR6050705.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            3875..5027
                     /locus_tag="OO433_002240"
                     /pseudo
     CDS             3875..5027
                     /locus_tag="OO433_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
     gene            5037..5805
                     /locus_tag="OO433_002241"
                     /pseudo
     CDS             5037..5805
                     /locus_tag="OO433_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5802..6059
                     /locus_tag="OO433_002242"
     CDS             5802..6059
                     /locus_tag="OO433_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCR6050706.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            6013..6984
                     /locus_tag="OO433_002243"
     CDS             6013..6984
                     /locus_tag="OO433_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCR6050707.1"
                     /translation="MVSGAALHITGFLRVKDSLRSLRILLDILLICLKGITVTKSISL
                     SKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNYHATIDGKEIAGITNNISSLTW
                     SAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKDLETIEYIGDNQFVISDERDYA
                     IYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYSRQDHTFWFFKEKNPIEVYKVN
                     GLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTLLVLSHESRALQEVTLVGEVIG
                     EISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNRFYRFTPKSSH"
     gene            7052..8224
                     /locus_tag="OO433_002244"
     CDS             7052..8224
                     /locus_tag="OO433_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6050708.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVIMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALARGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIALGNEYVLAGL
                     HFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAILYG
                     LAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPCLT
                     ANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLLGF
                     VTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS"
     gene            complement(8237..8791)
                     /gene="kptA"
                     /locus_tag="OO433_002245"
     CDS             complement(8237..8791)
                     /gene="kptA"
                     /locus_tag="OO433_002245"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6050709.1"
                     /translation="MAKHNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            9041..9724
                     /locus_tag="OO433_002246"
     CDS             9041..9724
                     /locus_tag="OO433_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050710.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9721..10182
                     /locus_tag="OO433_002247"
     CDS             9721..10182
                     /locus_tag="OO433_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6050711.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10195..11367
                     /gene="iadA"
                     /locus_tag="OO433_002248"
     CDS             10195..11367
                     /gene="iadA"
                     /locus_tag="OO433_002248"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6050712.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11432..12343
                     /gene="hypT"
                     /locus_tag="OO433_002249"
                     /gene_synonym="qseD"
     CDS             11432..12343
                     /gene="hypT"
                     /locus_tag="OO433_002249"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6050713.1"
                     /translation="MDDCCAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEYGEAIFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12336..12728)
                     /gene="iraD"
                     /locus_tag="OO433_002250"
     CDS             complement(12336..12728)
                     /gene="iraD"
                     /locus_tag="OO433_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6050714.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12725..12808)
                     /gene="idlP"
                     /locus_tag="OO433_002251"
     CDS             complement(12725..12808)
                     /gene="idlP"
                     /locus_tag="OO433_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR6050715.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            13400..14230
                     /locus_tag="OO433_002252"
     CDS             13400..14230
                     /locus_tag="OO433_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6050716.1"
                     /translation="MSIPLSNALQSQIITDNHFLHHPKVDNKLTREYEYTRMDTENIY
                     LLPLTRGNNHNYDGKSVVEIRKLNIGKQSWPFNYATDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGYNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(14371..15144)
                     /gene="uxuR"
                     /locus_tag="OO433_002253"
     CDS             complement(14371..15144)
                     /gene="uxuR"
                     /locus_tag="OO433_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6050717.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(15359..16819)
                     /locus_tag="OO433_002254"
     CDS             complement(15359..16819)
                     /locus_tag="OO433_002254"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6050718.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLKAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(16900..18084)
                     /gene="uxuA"
                     /locus_tag="OO433_002255"
     CDS             complement(16900..18084)
                     /gene="uxuA"
                     /locus_tag="OO433_002255"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6050719.1"
                     /translation="MEQTWRWYGPNDLVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            18424..18628
                     /locus_tag="OO433_002256"
                     /pseudo
     CDS             18424..18628
                     /locus_tag="OO433_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18871..>18983)
                     /locus_tag="OO433_002257"
     CDS             complement(18871..>18983)
                     /locus_tag="OO433_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6050720.1"
                     /translation="GTSAVSLGLTANYARTGGQVTAGNVQSIIGVTFVYQ"
BASE COUNT         4441 a         4812 c         4782 g         4948 t
ORIGIN      
        1 ttgtcattat tcgctccctg tggtgtggca ttcgcctggt aagttcgctg ataaacgcct
       61 taaaacgcgc ttcatcgcca gtcagtaaaa tgtaatttaa cagcgcagtt atctggctgt
      121 cattagcaca ctctgtaacc agtaatacta ccagttggtc aataagcccc atcaaatcgc
      181 gctggcgaat atgcttttgg atcaactcca acagggcgac tctgcgatgc tgcacaatct
      241 cgtcgtctgg cacgacagtg acatccacca gcgggaacgc tgcgctataa agtttccgtg
      301 cggtagtcgg ggcggcaaat tcgtccagcc agcacaggga ccaggggtaa ggactacggc
      361 taccgtgata aaacagcatc gggataacca gcggtagcgg ccggcgttta tcatgctcga
      421 tatggcgctg catcaccgcc atggaatatc gcatcaggcg aaatgccata tgaatatcct
      481 cgcggctctg atgttcaatc actacgtaaa tataaccatc accttcacgg gtctttaccg
      541 accacagaat atcggagtgt agcgcccgca atttttcatc gacaaagctg gcggattcca
      601 gttttaagct gtcgagatcg cacagttcac gtaaatcttt gggtaagtgg atctccataa
      661 aatcccgcgc ggtgtcaggg tgagtgagaa aggatttaaa taatgcgtca tgtggcgtgc
      721 tggtcgtgaa gtttgtcatg gcgatccgtc acctgtagat aagatggccc gactttagcg
      781 actaacaaaa ccattctcac ccggcaattt ctcgttcccc gaggtgcttt ccaggcgtct
      841 gcaaacgata catttttccg gaaacaggct cgcgttttac atttcctgta cccggcagat
      901 attccccttt ccaccgcgct cttttgatta accgcaaatt ttaattacac ttaaggtgta
      961 tattttctat gcaacccatc aattaaagag gtgtaatgtg ctgatgacta tttgtaatcg
     1021 ttatacattc tgacctggag tcagaaggta tttctctgtc tgtgtgttca caggcagtgt
     1081 ggttgattac atgaaattca gtacatttgc agtctcgttg cccttctcgc ctgcctttcg
     1141 tcattaccga cggtattgaa tttcgttttc cccgttgggg ttctccgaac aaggagttgt
     1201 ttgttatgcc acgttttttt gcccgccatg ccgccacgct gtttttcccg atggcgttga
     1261 ttttgtatga ctttgccgcg tacctgtcga cggatctgat ccagccgggg atcattaatg
     1321 tggtccgtga ttttaatgcc gatgtcagtc tggccccggc agccgtcagt ctctatctcg
     1381 cgggcggtat ggcgttacag tggctgctgg ggccgctttc cgacaggatt ggccgcaggc
     1441 cggtgctgat taccggggcg ctaatattta cccttgcctg cgctgcgaca atgttcacaa
     1501 cgtctatgac acagtttctt atcgcgcgtg caattcaggg caccagtatc tgttttattg
     1561 ccaccgttgg ttatgtcacg gtgcaggagg cgttcggcca gacaaaaggg atcaagttga
     1621 tggcgattat cacctccatc gtactgattg cgccgattat tggcccgctt tccggcgcag
     1681 ctctgatgca ctttgtgcac tggaaagtcc tttttgccat cattgcagtt atgggtttta
     1741 tctcatttgt tggcttactg ctggcgatgc cagagacggt gaagcgcggc gcggttccat
     1801 ttagcgccaa aagcgtcttt cgcgattttc gtaatgtctt ttgcaatcgg ctgttcctct
     1861 ttggcgcagc aaccatctct ttaagctata tcccaatgat gagttgggtg gctgtctcgc
     1921 cggtgatcct gatcgacgcg ggcagcttaa caacttcgca gttcgcctgg acacaggttc
     1981 cggtgttcgg cgcggtgatt gtcgcgaatg ccatcgtggc gcgtttcgtt aaagatccga
     2041 ccgaaccgcg ctttatctgg cgtgctgtac ccattcaact ggtcggcctc gcgctgttga
     2101 ttatcggcaa tctgctgtca ccgcacgtct ggctgtggtc ggtgctgggc accagtttgt
     2161 atgctttcgg gattggtttg atcttcccga ccttattccg ctttacgctt ttttccaata
     2221 acttaccgaa agggaccgtc tccgcatcgc tgaatatggt gatcctgatg gtgatgtctg
     2281 tctcggtcga aatcggccgc tggctgtggt ttaacggcgg tcgcttgccg tttcatctgt
     2341 tagccgtcgt ggcgggcgtt atcgtcgttt tcaccctggc aggattgctt aatcgcgtgc
     2401 gccaacatca ggctgccgag ctggcggagg agcggtgatt tttgcgcgat cctgccgtca
     2461 ggctctattc ttaacgttat gaataaactc attgaactca gacgcgccaa aatgttggcg
     2521 ctctctttac tgcttatcgc cgctgctacc tttgtcgtta cgctgttttt gccgcccaat
     2581 ttttgggtga gcggcgtgaa ggcgattgct gaagcggcga tggtcggcgc gctggcggac
     2641 tggtttgcgg tggtggcgct gtttcgccgc gtgccgattc cgatcatttc tcgtcatacg
     2701 gcgattatcc cgcgtaataa agaccggatt ggcgaaaatc tcggtcagtt cgtgcaggaa
     2761 aaatttctcg atacccagtc gctggtggca ttgattcgac gtcacgaacc ggcgttgttg
     2821 attggcaact ggtttagtca gccggagaac gcccgccgcg ttggtcagca tctgttgcag
     2881 atcatgagcg gttttcttga actgaccgat gatgcgcgta ttcagcgcct gcttaagcgc
     2941 gcggtccatc gggcgattga taaggtcgat ctttccggaa ccagtgcgtt gatgctggag
     3001 agtatgacca aaaacgatcg tcatcaggtg ctgctcgata cgctgatcgc acagttgatc
     3061 gcccttctcc agcgcgataa atcgcgcaag tttattgccc agcaaattgt tcgctggctg
     3121 gagagcgagc atccgctgaa agccaaaatt ttgcctactg aatggctggg cgaacatagc
     3181 gcggagttgg tttctgacgc ggtgaattct ttgcttgatg atatcagccg cgatcgtgcg
     3241 catcagatcc gtcatgcgtt tgatcgcgcc acttttgccc tgatcgacaa gctgaaaaac
     3301 gatccagaaa tggcagcgcg agccgatgcc gtaaaaagtt atctgaaaga agatgaagct
     3361 tttaaccgct atctcagtga attgtggggg gatttacggg aatggctgaa agcggatatc
     3421 aacagtgaag attctcgtgt gaaagaacgt atcgcgcggg cgggtcaatg gtttggcgaa
     3481 acgttaattg ccgatgatgc cttgcgggca tcgttaaatg gtcacctgga acaagccgcg
     3541 caccgcgtcg cgcctgagtt ttccgcattc ctgacgcgcc acatcagcga tacagtaaaa
     3601 agctgggatg ctcgggatat gtcgcggcaa atcgagttaa atatcggcaa agatctgcag
     3661 tttatccgtg tcaacggtac gctggttggc ggttgtattg ggctaatttt atatttgttg
     3721 tcgcagctcc cggccttgtt ccccctcagc aatttttaga aatccatgaa aaactaagtg
     3781 gctaaccgcg agagagatca aataaaccac actaaaagaa tggtaataat gtggcctctt
     3841 ttcattatct cccgtggtac ggggaaggaa aatcatgtca cttatcaccg atctacccgc
     3901 cattttcgat cagttctctg aagctcgcca gaaaggcttt ctcaccgtca tggatctcaa
     3961 ggagcgcggc attccgctgg ttggcactta ctgcaccttt atgccgcaag agatcccgat
     4021 ggcagccggt gcggttgtgg tttcgctctg ttctacctct gatgaaacca ttgaagaagc
     4081 ggagaaagat ctgccgcgca acctctgccc gctgattaaa agcagctacg gcttcggcaa
     4141 aaccgataaa tgcccctact tctacttttc ggatctggtg gtcggtgaaa ccacctgcga
     4201 cggcaaaaag aaaatgtatg aatacatggc ggagtttaag cccgttcatg tgatgcagtt
     4261 gccgaacagc gttaaggacg atgcctcgcg tgcgttatgg aaagccgaga tgctgcgctt
     4321 gcaaaaaacg gtagaagaac gttttgggca cgagattagc gaagatgctc tgcgcgatgc
     4381 cattgcgctg aaaaaccgcg aacgtcgcgc actggccaat ttttatcatc ttgggcagtt
     4441 aaatcctccg gcgcttagcg gcagcgacat tctgaaagtg gtctacggcg caaccttccg
     4501 gttcgataaa gaggcgttga tcaatgaact ggatgcaatg accgcccgcg ttcgtcagca
     4561 gtgggaagaa ggccagcgac tggacccgcg tccacgcatt ttaatcaccg gctgcccgat
     4621 tggcggcgca gcagaaaaag tggtgcgggc gattgaagag aatggcggct gggttgtcgg
     4681 ttatgaaaac tgcactgggg cgaaagcgac cgagctaatg cgtggcggaa acgggcgatg
     4741 tctacgacgc gctggcggat aaatatctgg cgattggctg ctcctgtgtt tcgccgaacg
     4801 atcagcgcct gcaaatgctc agccagatgg tggaagaata tcaggtcgat ggcgtagttg
     4861 atgtgatttt gcaggcgtgc catacctacg cggtggaatc gctggcgatt aaacgtcatg
     4921 tgcgtcagca gcacaacatt ccttatatcg ctattgaaac agactactcc acctcagatg
     4981 tcgggcagct cagtacccgt gtcgcggcct ttattgagat gctgtaagga gtggcagtga
     5041 catattcgat tggcattgat tctggttcta ccgccaccaa agggatctta ctggcagacg
     5101 gcgtgattac gcgccgtttc ctcgttccaa caccctttcg tccggcaaca gcaattactg
     5161 aagcctggga aactctgcgc gaagggttag agacaacgcc gtttctgacg ctcaccggct
     5221 atggacggca actggtggat tttgccgata aacaagtgac ggaaatctcc tgtcacgggc
     5281 tgggcgcacg gtttcttgcg ccagcaacgc tgcgcggtaa tcgacatcgg tggtcaggac
     5341 agcaaagtga ttcagcttga tgatgacggt aacctgtgcg atttcctgat gaatgacaaa
     5401 tgcgcggcgg gcaccgggcg tttcctggaa gtgatctcgc gcacgcttgg caccagcgtc
     5461 gagcaactcg acagcattac cgaaaatgtc acgccgcatg ccatcacgag tatgtgcaca
     5521 gtgtttgccg aatcagaagt gatcagcctg cgctcagcgg gcgtcgcgcc agaagcgatt
     5581 ctcgccggag tgattaacgc gatggcgcgg aggagtgcca atttcattgc tcgtctctcc
     5641 tgtgaagcgc cgattctgtt tactggtggc gttagtcatt gccagacgtt tgcccggatg
     5701 ctggaatctc acctgggaat gccggtaaat acccatcctg atgcgcaatt tgctggcgca
     5761 attggcgcgg cggtaattgg tcaacgagtg aggacacgcc gatgaaagag tttttatttt
     5821 tatttcactc cacagtcggc gtcatacaaa ctcgcaaagc gttgcaggca gcgggcatga
     5881 cttttcgcgt cagcgatatt ccccgtgatt tacgcggcgg ctgcgggtta tgcatttggc
     5941 tgacctgtcc ccccggcgaa gaaatacaat gggtgatccc tgggctgact gagtcaattt
     6001 attgccagca ggatggtgtc tggcgctgca ttgcacatta ccgggtttct ccgcgttaag
     6061 gattcattaa ggtcgctacg tatacttctc gatatcttat tgatttgcct taaagggatt
     6121 accgtgacaa aaagtatttc gttaagtaaa cgcatctccg ttattgtgat tctttttgcc
     6181 attgtggcag tgtgtacttt cttcgtgcaa tcttgcgccc gtaaaagcaa tcatgcagca
     6241 tcatttcaga attaccacgc caccattgac ggcaaagaaa ttgcaggcat aacgaataat
     6301 atctcttcgc tgacctggtc ggcacaaagc aatactcttt ttagcactat caacaaacct
     6361 gccgccattg ttgaaatgac caccaatggt gattttatcc gcacaattcc tctggatttt
     6421 gttaaggatc tcgagacgat cgaatacatt ggcgataatc agtttgtcat tagcgatgag
     6481 cgcgattacg ccatttatgt gatttcactg accccaaact cggaagtaaa aatcctcaaa
     6541 aaaataaaaa tcccgctgca agactcccca accaattgcg gttttgaagg gttagcttat
     6601 tctcgccagg atcacacttt ctggtttttt aaagagaaaa atccgattga ggtctacaaa
     6661 gttaacgggc tgttaagtag caatgaatta cacatcagca aagacgaggc actacaacgg
     6721 caatttactt tagatgatgt gtccggcgca gaatttaacc agcaaaaaaa tacgcttctg
     6781 gtgctgtctc atgaatctcg cgcactccag gaagtaaccc tggttggcga ggttatcggt
     6841 gaaatatcac taaccaaagg tagtcgaggt ttgtcgcata acatcaaaca ggccgaaggg
     6901 attgcgatgg atgcctctgg taatatttat atcgttagcg aaccaaatcg tttttatcgc
     6961 tttaccccta aatcatcgca ttaacaggct ggcatatttc acctttttgt tattaacttg
     7021 ttaacaacaa aaatgacata caaggttaaa catgccttcg tcctcgcatc ccgtagaacg
     7081 cttttctttc agcaccgcgc ttttcgggat gctggttctg accctaggta tgggattagg
     7141 tcgcttcctt tatacgccta tgttgcccgt cattatggcg gaaggggcgt tttcatttag
     7201 ccagctctcg tggattgcca gcggcaacta tgcggggtat ctggctggca gtctgctatt
     7261 ttcgtttggc gcatttcacc agccgtcgcg cctacgccca tttctgttgg tttctgccct
     7321 ggcgagaggg ttgttgatcc tcgcaatggc atggttgccg ccatttattc tggtgttatt
     7381 gattcgcttc ctggcgggtg tcgccagcgc cggtatgctg atttttggtt cgacgctgat
     7441 tatgcagcac acgcgccatc cttttgtgct ggcagctttg ttttctggcg ttggcattgc
     7501 actgggcaac gaatatgttc tggcaggcct gcattttgcc ttctcctcgc aaacgttatg
     7561 gcaaggcgcc ggggcgcttt ctggcatgat gctgatcgca cttacgcttt taatgccctc
     7621 gaaaaaacac gccatcgcac caatgccatt ggcaaaaacg gagcaacaga taatgagctg
     7681 gtggttactg gctattctgt acggccttgc gggatttggt tatatcatcg tcgcgactta
     7741 cctgccgctc atggcgaaag atgcaggctc accattgtta accgcccatc tctggacgtt
     7801 agtcggctta tcaattgtgc caggctgctt tggctggcta tgggcagcaa aacgttgggg
     7861 ggctttgcct tgcctgacgg cgaatttgct ggtgcaggct atcagtgtgc tgcttactct
     7921 cgccagcgac tctcctctct tgcttatcat tagtagtatc ggttttggcg gtacctttat
     7981 gggcacgaca tctctggtga tgactatcgc ccgccaactg agcgttccgg gaaatctcaa
     8041 tcttttaggc tttgtgacgc tcatttacgg cattggacaa atcctcggcc cggcgctgac
     8101 cagtatgctc ggcaatggaa catcggcgct tgccggcgcc actctctgcg gcgcggcggc
     8161 gttgtttatt gctgcgctta tcagcacagt acaattattt aagctgcaag tggtcacttc
     8221 atgaggagtg tttcccttac cactcaagaa attcgaccgc aaccgttgat gtcagccaga
     8281 ctccgttttc cgcttgccag aaaggaatgc ctcgtttcgc catttcctgt gctttgacgg
     8341 ttaagacaac cggcgatccg tgtcttgctc ccactttgcg tgccgttgct tcatcagcag
     8401 agagatgcac gtaatgacga tcccctgcaa tcagcccctg tttttttatc tcgtcaagga
     8461 aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt tttttcggca aaggaaatag
     8521 cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca cctaccatca ctggaataac
     8581 taaaacgctt tttatcgctg gttgcgacca cggtatccag caatgcacgg gttaatcgct
     8641 tgcccgcttt ttgggcacaa agtatgagct tatcgatatc cgcccatcct tcacggtcca
     8701 gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa acttaaaaac ttgctcgtat
     8761 ccgccagctc tttttcgttg tgctttgcca tctcgttttc gtccctggat tttctgtttt
     8821 tatttaccaa tgtcattgtc actgggttta tgaattttct gcaactcaat cctgcgcaga
     8881 accttttcca cacagcgtaa tgtttcccct atgcaaaaaa cgcatagcca gaaacatact
     8941 tgcattttcc ccatacccac atggctgata aggtttttca gttccaggaa ttttcctggc
     9001 tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt atgacacaac aaggggatgc
     9061 tgtagccggg gagctggcga cagaaaaagt gggcattaaa gggtatctgg ctttttttct
     9121 caccataatc tttttttccg gtgttttttc cggaacagac agctggtggc gcgtttttga
     9181 tttttccgtt ctgaacggat cgtttgggca actgcctggt gcgaacggag ccaccacctc
     9241 atttcgcggc gcgggtggtg cgggggctaa agatggcttt ctctttgcgc tggagctggc
     9301 accgtcagtc attctctctc tggggattat ttcaattacc gatggtcttg gtggattacg
     9361 cgccgcgcag cagttaatga cacctgtgct aaaaccgttg ttgggcattc cagggatctg
     9421 ctcgctggcg ctgatcgcta acttgcaaaa cactgatgcc gccgccggta tgaccaaaga
     9481 gctggcgcag gaaggtgaaa ttaccgaacg cgataaagtg attttcgccg catatcaaac
     9541 cagcggtagc gccatcatca ccaactactt ctcttccggc gtcgcggtgt tcgcctttct
     9601 gggcacgtcc gtaatcgtac ctttagccgt catcctggtg tttaaatttg tcggagccaa
     9661 cattttgcgc gtctggctta acttcgaaga acgtcgcaac ccaacgcaag gagcgcaagc
     9721 atgacaactc aggtccgcaa aaacgtcatg gatatgttta tcgatggcgc gcgccgtggc
     9781 tttactatcg ccacgaccaa cctgctgcca aacgtggtga tggcgtttgt catcattcag
     9841 gcgctgaaaa ttaccggcct gctcgactgg gtggggcata tctgtgaacc cgtaatggcg
     9901 ctgtggggat tacccggtga agcggcaacc gtccttttag ccgcattaat gagtatgggg
     9961 ggcgcagtcg gtgttgccgc cagtctggca accgctggcg cattaaccgg tcacgatgtc
    10021 accgttttac tgcccgcgat gtatttaatg ggcaacccgg tgcaaaatgt cggtcgctgc
    10081 ctggggacag ccgaagtgaa tgccaaatat tacccgcata tcattacggt ctgcgtgatt
    10141 aacgcattgc tgtcgatctg ggttatgcag cttattgttt aacaaggagt taccatgatt
    10201 gattataccg cagccggttt taccctgctg cagggagcgc atttgtatgc gccggaagat
    10261 cggggaattt gcgatgtcct cgtcgctaac ggcaaaatta tcgccgttgc cagcaatatc
    10321 ccttctgaca ttgtaccgaa ctgcacggtt gtcgatctca gtgggcagat cctctgccca
    10381 ggttttattg atcaacacgt ccatttgatt ggcggtggcg gcgaagcagg tcccacgacg
    10441 cgcacgccgg aagtggcgct aagtcgcctg acggaagcgg gcgtcacgtc agtggttggt
    10501 ctgctgggca ccgactctat ctctcgccac ccggaatccc tgctcgccaa gacccgtgcg
    10561 ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg cttatcatgt cccttcccgc
    10621 actattacgg gttccgtgga aaaagacgtg gcgattatcg atcgtgtgat tggcgtgaaa
    10681 tgcgccatct ctgatcaccg ttctgccgca ccggacgttt atcacctggc taatatggcg
    10741 gcagaatccc gcgttggcgg tttgctcggc agtaaacctg gcgtcaccgt gttccacatg
    10801 ggcgacagta aaaaggcgtt acagcctatt tatgacctgc tggaaaactg cgatgtgccg
    10861 atcagcaagc tgctgccgac ccacgttaac cgcaacgtac cgttgtttga gcaggcgctg
    10921 gagttcgcgc gcaaaggcgg caccatcgat atcaccagca gcattgacga accggtcgcc
    10981 cctgccgaag gtattgcccg cgccgttcag gcgggtattc cgctggcacg cgtcaccctc
    11041 agctccgacg gcaacggtag ccagccgttc ttcgatgacg aagggaattt aacccatatc
    11101 ggtgttgcag gttttgaaac gttgctggaa accgtgcagg tgctggtcaa agactatgat
    11161 ttcagtatca gcgatgccct gcgcccgctc accagtagcg tagccggttt ccttaacctg
    11221 agcgggaaag gcgaaattct gcctggcaat gacgcagact tactagtcat gacgccagaa
    11281 ttgcgcattg agcaggtata tgctcgcggc aaactgatgg tcaaagacgg caaagcctgc
    11341 gtgaaaggaa cgtttgaaac ggcttaaacg gttgtcagcc gccctaatgg acgtagaatg
    11401 cccgcaaggg cggctgacag agtaaaacgt aatggatgac tgttgtgcgg ttttgcataa
    11461 tattgaaacc aaatggcttt atgattttct gaccctggaa aaatgccgca atttttccca
    11521 ggcggcagtc agtcgtaacg tctcgcaacc ggcattcagc cgccgcatcc gcgcgctgga
    11581 acaggcgatt ggcgttgaat tgtttaaccg ccaggtgacg ccgctgcaac tctcagaaca
    11641 agggaaaatc ttccattcgc agatccgcca tctgttgcaa cagttagaaa gtaaccttgc
    11701 agagctgcgt ggcggcagcg attacgcgca acgtaaaatc aagatagccg ctgcacactc
    11761 tctttccctc ggtctgttac cgtccattat cagccagatg ccgccgctct ttacctgggc
    11821 gattgaagct attgatgtcg atgaagcagt cgataaactg cgtgaagggc aaagtgactg
    11881 tattttttcc tttcacgacg aagatttgct ggaagcaccg tttgaccaca ttcgcttatt
    11941 tgaatctcaa ttgttccccg tttgcgccag tgacgaatac ggagaagcaa tttttaacct
    12001 cgcgcagcca cactttccgt tactgaatta cagccgcaac tcctacatgg ggcgattgat
    12061 taatcgcacc ctgacgcgcc acagcgagtt aagtttcagc accttttttg tctcttcgat
    12121 gagcgagcta ttaaagcagg ttgccctcga cggctgtggg attgcctggc tgccggagta
    12181 tgccatccaa caagaaattc gcagcgggca actcgttgtg ctgaatcggg acgaactggt
    12241 gatcccgatt caggcctacg cataccgtat gaatacccgc atgaatccag ttgccgaacg
    12301 cttctggcgt gaactgcgcg agctggagat tgtgcttagc tgacattctc aagcgtcgca
    12361 ctgcgccaac cgccattatc ccagaccaga tcaaacgtta gcggttcatc gtcgtggtac
    12421 agcattatct caaggctgat cgccagagtg tggcagtacg cttctttcaa attaacctgc
    12481 aacgccgcaa tccgtggctc atgaaataac agtgcagaac gcagctgacg gcaataccag
    12541 ctctggagat gtttgtcgct cgggttatgc acttgccact cgggcatgcc tgaaacaagt
    12601 aatgacggta atgccgaatg aggagaatta aaaagagtga ggagatcgct gcataccgat
    12661 ttacgcagca atgacgtttt attgcctgaa atttcaggta aaacagcctg aagtgattgg
    12721 cgcatcattt tgcgcactcc tgacgtttag caacatatgc ggcttgccct gaacaccggg
    12781 caccactgta ttgatgctca ttttccatag ccattccctt ttgttcatta catccatgtc
    12841 agtctgagac aggcctgaat cctgtcagca aagtataaaa ccttacttgc ctatgaatat
    12901 ctactgactt ttcaggaaaa aagtcatgat ttgataaaaa tcatcaaact caaaaaaaaa
    12961 ccgctagtat attctgaata ttccgaatag ttacgtagat gaaaatactt gtcaccgccg
    13021 ctacttaatt ccggtggaaa attatttatt tccgcataat cagccatgta ttatttgaaa
    13081 caattagttt gtaaataata taaattgata acatatcaaa tacatcaaaa ataccattca
    13141 taggcattat taatatagac atcaatatag caacatcaaa ttccaattag taataagata
    13201 tgctttttaa ttgcatgaaa ataatttcaa atgagcgttt tttgatactt tagagtgttt
    13261 cctacccttt aatatcaccg tgtagagttg atatcactac aacagaaaca tgcatgtcat
    13321 ttttttactt cttttttact acagaaaaat ttatctattt ttctaaacat cggtgaaata
    13381 ctcataaaag gtaactatta tgtctatacc attaagcaat gcccttcaga gtcaaattat
    13441 tacagataat cacttccttc atcatccaaa agtcgacaat aagttaacac gtgaatatga
    13501 atatacacgc atggatacgg aaaatatcta tcttctgcct cttactcgtg gaaataacca
    13561 taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt aatattggca aacagtcctg
    13621 gccatttaat tatgcgacag atacatgcag agaattcaat ggtattacaa caacaggacg
    13681 tatgctctat cgaaacttga aaatcacctc agctttagac gaaatatatg gtggcatatg
    13741 taaaaaggcg catgctgcta cagagttagc agaaggttta cggctaaatt tatttatgaa
    13801 gagccctttc gatcctgttg aggactatac agttcatgaa ataacattag gccctggtta
    13861 caatgttcct ggttatgccg gaaccacgat aggatatatt tcaacccttc cagcttctca
    13921 ggcaaaaaga tggaccaatg agcagccaag aattgatatc tatattgatc agataataac
    13981 tgtctcaggg gtggcaaatt cttccggatt cgcccttgcc gcattactta atgctaatat
    14041 tggaatggga aatgacccaa taattggtat agaagcctat cctggtaccg cggagatcca
    14101 tgcaaaaatg ggatataacg ttatccccgg tgatgaagat gcgccactca aacgcatgac
    14161 tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa aatggtgaat ggaattatat
    14221 tggcaaataa ccactgagaa gaatgagtac taacattcag gttatataga tatttaaaca
    14281 ataccctgca atttacaaag taatactcat tgcgacttct taatttaaga aagcgcaagg
    14341 aacgtttacc cttgcgctta tcataatcag tcaggcgtcg actttatcca gcggccatga
    14401 atcaaacaga tagccgtcaa aataaatatc gtcaacgttc gagaattcca gcagacgttg
    14461 cttaacgttt tccagatgct gccacattgc cagcttcgcc gctcgggcat cttttttgat
    14521 taacgcggca aggatctgtt tgtgatcgcc caaccactct ttgcgataca ggctgtcatc
    14581 cagatggctg tgcaactgga tccacattgg attgttttcc cgccactgcc aggactgacg
    14641 gaataactcc accagcatgc tgttatgcgt tgcttcagca atggcgagat ggaactgcat
    14701 gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt tcacgctctt ccagttgcaa
    14761 tgcctgacgc attttgacga tatcttcgcg ggtagcctgc agggcggcaa attccgcgat
    14821 gttgctctca agaagctgtc gcgcctgcaa caattcaaag gggccggcat cattgcagac
    14881 gttggcatcc ggactgtctg tgttgtggct gcctgagcta tcaagaacat agataccggc
    14941 accccggcgc acttccacca gccctttgat ctccagcatg atcagcgctt cacgtaccac
    15001 cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc ggcggcagcc gttcgccagg
    15061 attgtacggc gtctttatga tcagatcgcg gatcatcgcc ccgacttcct ggtaaggtct
    15121 ttgcgcagag gtggcagatt tcatggtgtt gtgtccgtta ggtgcggtta atcaaatttt
    15181 tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg gcaattttac ggcccccgtt
    15241 aatctcaaac tataccatta gcactggaag ttggcacgta caggtgggca aaaaaacgaa
    15301 aactggtcaa ccaaatatca gtttgattga ccagtaagtc tgtagggtaa tcagttagtt
    15361 acagcgcagc cacacactcg cgcgcaccgc gttcgcacag ctgctgatat gccgccgtca
    15421 ccgcgccaac aaagtcggca ttctgcggca gatcatcggc aaaaataccg ctcaggccca
    15481 gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt aatcttctgg aactcggcca
    15541 gcatcgggtc gaccacgtca atggcattac cctgctcatc cacgccctgg gtgtaacgca
    15601 tccagccagc cacgcccagc gccaggtgac gccagctgcc gccgttttgc aggtgcagac
    15661 gcaccgggtc cagcagacgc tgcggtaact tctggctgcc gtccatcgca atctgccagg
    15721 tacggtgacg cagagacggg ttgctgaaac gctcgatcag cagcgtcgca taggccttca
    15781 ggtccgtacc ctccggcata gacagcgttg gcgcttgttc ctgcatcatc agggcaaagg
    15841 ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat agtttcatag ccgccgaggt
    15901 aaccgaggta tgccaggaaa gagtggctgc cgttcagcat acgcagcttc atcatttcga
    15961 acggcacaac gtctgcaacg aactgtgcgc ccactttatc ccagtccggg cgaccattaa
    16021 cgaagttatc ttcaatcacc cactgacgga acggttcgca ggcaatggcg cacgggtcgt
    16081 aaacacccag ctggtcagca atttcctgta aggtttctgg cgtcgccgcc ggaacgatgc
    16141 ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat ccatgccgcc agctgcgggt
    16201 cacgtgcctg agccagcccc agtaccgcga ccttcgccac atgaccgttt tcacgcacgt
    16261 tatcgcagga catcaccgta aatgctttca gccctttttc acgacgcaga cgcaaggctt
    16321 cgacgatgta accaatcgcg gacttcggcg cagtcgggtt ttctagatcg tgcttgatca
    16381 gcgggttatt gagatccagt tgaccgcttg ccgcatcagc gcagtagcct ttttccgtga
    16441 ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc gttgagaata ccttcgcagc
    16501 cgtcgatttc cggatgcagc gcttctttca tcgaaccgat aattttcagc tcggtgcttt
    16561 ctgcgccttt ttccgctacg gtgtacagca gttgctgttt tttcaggttt tcgatcagca
    16621 cgcggtcgtt gcctggcatc aggttaactt cgcagatgcc ccagtcgctg tcggtgcttt
    16681 ccagcagatg atgggtatac agcgcctgat gtgcgcggtg aaacgccccg caaccgagat
    16741 gcacaatgcg tgattccaga cgagaatgat cccatgacgg acgggcaacc ggcagattgc
    16801 tgtcaacaat agtagtcatc gtaaactcca gaatccgtct tcagagagtg acggcaagga
    16861 agaaggaacc ggcacggcgc gcagccatgc cggtggatat taacggctaa agaaagcgcg
    16921 ctggatcgcc agttcgacac cgcgaacttc ggccaggcct ttcagacgac caattgcgga
    16981 gtaacctggg ttggttttct tcttcaggtc gtccagcatc tgatgaccgt ggtccggacg
    17041 catcgggatc aggtcttctt tgccttccgc tttacgacgg tgttcttctt caacaatcgc
    17101 tttcaccact tcgtacatat caacgtcacc gttcaggtgc gccgcttcgt ggaaggtttt
    17161 cgggttatct tcacgcatgg tggagcgcag atgggtgaag taaatacgcg gaccgaactg
    17221 cttgatcata tcaaccagat cgttgtcagc acgcacgccg taggaaccgg tgcacatggt
    17281 gaaaccgttc gccatgctgt ttacggtatc aaccatccac tgcatatctt caatggtgga
    17341 aacaatgcgc ggcaggccga ggatcgggcg cggcggatcg tccgggtgaa ccgccatacg
    17401 cacgccaact tcttcagcaa ctggaataat cgctttcagg aagacggcaa agttttcgcg
    17461 cagcttcgct ttatcgatat ctttgtacag ctccaggtgc ttacggaact ggtcgagggt
    17521 atacccttct tccgcgcccg gcagaccggc aatgatgtta cgcgtcagac gcgctttgtc
    17581 ttcatcgctc atagtggcga agcgttcagc tgcctgagca atttcttctt cggtgtaatc
    17641 cgcttccgcg cctggacgtt tcaggatatg catttcgaat gcagcgaatt cgatctggtc
    17701 gaagcgcaga gctttggagc cgtctggcag cacgtattcg aggtcagtac gggtccagtc
    17761 gagcaccggc atgaagttgt agcacacggt gcgaatgccg cactgcgcca ggttgcgcag
    17821 agtctgctga tagttagcga tccactgctc atagttgcca gtgtgggttt tgatatcttc
    17881 gtgaattggt acgctttcta cgacagacca caccaggcct gcgtcttcaa cgatcgcctt
    17941 gcgtttgagg atctcttcta cggaccatac ttcgccgttc gggatatggt gcagcgcggt
    18001 aaccacgcca gttgcgcccg cctgacggac atcagctaaa gaaaccagat cgtttgggcc
    18061 gtaccagcgc caggtctgtt ccatgatttt tcctctttca catagtgggt tccacagcaa
    18121 agagacgcgg aaccctggag ttgtttaggt atccggatga acaaccagac cgcagccgtg
    18181 gcgttgcatc ggatgagatg gcgtataagt tctacctttt ggtaagatat gtcaacccaa
    18241 atcctgaaat tggttaacca aatcacaaga atttcactct catggttgtt gctgcaaatt
    18301 gcagcaaaag gtaatcgatg gttattgatc agaggatgtg acattcatcg caacaatggt
    18361 tgaccaattt acataacatg tcgcccaaaa taacaacggt tcaaccaggt aaggtaaaac
    18421 gttatgcatg tgcttaacat tctctgggta gtattcggca ttggtctgat gctggtactg
    18481 acaacgaccg caggttcaga cacattcact gcattagcaa tgcctcttag attattttga
    18541 cctgtcagta aaagacacgt tgaaaacctg gggtctgctg gaactggtca actccgtggt
    18601 tgggctgatt attagcaagg tagcgtaaaa gaacaccaga gcctgccaat ggcaggctct
    18661 gactgatgac aaagtctaaa aatgtgcccg gacagtcccc tcgcccctcc ggggagaggg
    18721 ttagggtgag gggaacagac cagcaaagtg cgctttgttt atgccagatg cgacgctgac
    18781 gcgtcttatc tggcctacaa agggctaacg tgcaggtttt tagcttcagg taatattgcg
    18841 taactgcatt agcaatgccc tgtgatttct ttattgataa acaaaagtca cgccaataat
    18901 cgattgcaca ttccctgcag tcacctgccc tccggtacgt gcgtaatttg ccgttaatcc
    18961 cagacttacc gccgaagttc cta
//
LOCUS       DAJTSL010000073        18876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTSL010000073 DAJTSL010000000
VERSION     DAJTSL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.073"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..731
                     /gene="rihB"
                     /locus_tag="OO433_002258"
     CDS             <1..731
                     /gene="rihB"
                     /locus_tag="OO433_002258"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6050721.1"
                     /translation="MRKQIVADNIHGETGLDGPVFESLTRQAESTHAVKYIIDTLMAS
                     DGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPEA
                     ARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFENY
                     GLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTKV
                     GITIDTDWFWGLVEECVRGYIKTH"
     gene            831..2080
                     /locus_tag="OO433_002259"
                     /pseudo
     CDS             831..2080
                     /locus_tag="OO433_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(2136..3224)
                     /locus_tag="OO433_002260"
     CDS             complement(2136..3224)
                     /locus_tag="OO433_002260"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6050722.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGIADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGAGDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3227..4084)
                     /gene="nfo"
                     /locus_tag="OO433_002261"
     CDS             complement(3227..4084)
                     /gene="nfo"
                     /locus_tag="OO433_002261"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6050723.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4158..5207)
                     /locus_tag="OO433_002262"
     CDS             complement(4158..5207)
                     /locus_tag="OO433_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6050724.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIIVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5307..6188
                     /locus_tag="OO433_002263"
     CDS             5307..6188
                     /locus_tag="OO433_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6050725.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANIPR"
     gene            6393..7862
                     /gene="lysP"
                     /locus_tag="OO433_002264"
     CDS             6393..7862
                     /gene="lysP"
                     /locus_tag="OO433_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6050726.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8155..10135
                     /gene="cirA"
                     /locus_tag="OO433_002265"
                     /pseudo
     CDS             8155..10135
                     /gene="cirA"
                     /locus_tag="OO433_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(10167..11003)
                     /gene="yeiG"
                     /locus_tag="OO433_002266"
     CDS             complement(10167..11003)
                     /gene="yeiG"
                     /locus_tag="OO433_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6050727.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11261..11929
                     /gene="folE"
                     /locus_tag="OO433_002267"
     CDS             11261..11929
                     /gene="folE"
                     /locus_tag="OO433_002267"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6050728.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11946..13103
                     /locus_tag="OO433_002268"
     CDS             11946..13103
                     /locus_tag="OO433_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6050729.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            13245..14285
                     /gene="galS"
                     /locus_tag="OO433_002269"
     CDS             13245..14285
                     /gene="galS"
                     /locus_tag="OO433_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR6050730.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14565..15563
                     /gene="mglB"
                     /locus_tag="OO433_002270"
     CDS             14565..15563
                     /gene="mglB"
                     /locus_tag="OO433_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6050731.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQSVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15624..17144
                     /gene="mglA"
                     /locus_tag="OO433_002271"
     CDS             15624..17144
                     /gene="mglA"
                     /locus_tag="OO433_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6050732.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDKSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17160..18170
                     /gene="mglC"
                     /locus_tag="OO433_002272"
     CDS             17160..18170
                     /gene="mglC"
                     /locus_tag="OO433_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6050733.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPCWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18413..>18876)
                     /locus_tag="OO433_002273"
     CDS             complement(18413..>18876)
                     /locus_tag="OO433_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6050734.1"
                     /translation="IGGIETWEDAAEFLLLGAATLQVTTGIMQYGNRIVEDMASGLSH
                     YLADQGFDSLQEMVGLANNNIVPAEDLDRSYIVYPRINLDKCVGCGRCYISCYDGGHQ
                     AMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGCIELGEVKFKKGEKEHPVTL"
BASE COUNT         4506 a         4648 c         5016 g         4706 t
ORIGIN      
        1 ttatgcgtaa acaaatcgtt gccgataata ttcacggtga aaccggactg gatggtccgg
       61 tattcgagtc gctgacccgc caggcagaaa gtacccacgc ggtgaaatac atcattgata
      121 ccctgatggc aagcgatggc gatatcactc tggtgccggt tggtccgctt tcaaatatcg
      181 cggtggcaat gcgtatgcaa cccgcgatcc tgcccaaaat ccgtgaaatt gtgctaatgg
      241 gcggcgctta cggtacaggc aacttcacgc catctgccga gttcaacatc tttgctgatc
      301 cggaagcagc ccgcgtggtg ttcacctccg gcgttccatt agtgatgatg ggcctcgatc
      361 tcaccaacca gactgtttgc accccggacg tgattgcacg gatggaaagg gcaggcggcc
      421 ctgccggaga gctgttcagc gacatcatga acttcactct caaaacgcag ttcgaaaact
      481 acggcctggc tggcggcccg gtgcacgacg ccacctgcat cggttatctg attaaccctg
      541 atggcattaa aacccaggag atgtacgtcg aagtagacgt caacagtggc ccttgctatg
      601 ggcgtaccgt ctgcgacgag ctgggcgttc ttggcaagcc cgctaatacc aaagtcggca
      661 tcactattga taccgactgg ttctggggct tagtcgaaga gtgcgtgcgc ggctacatca
      721 aaacccatta agtgttgata acaagccggg tcagtatcgc aagatccaag cccggctatt
      781 tctgatgggc agaaagccgt cgcccaatat gacatgaaga gataataact atggatgtca
      841 tgagaagtgt tctgggaatg gtggtattgc tgacgattgc gtttttgctg tcagtaaaca
      901 agaagaagat cagcctgcgt accgttggcg cggcgttagt gttacaggtc gtgattggcg
      961 ggattatgct ttggttaccg gcagggcgtt gggtcgctga aaaagtcgct tttggcgtgc
     1021 ataaagtgat ggcgtacagc gacgcgggta gcgcatttat cttcggttcg ctggtcgggc
     1081 cgaaaatgga cacgctgttt gatggcgcag gatttatctt tggtttcagg gtattaccgg
     1141 caattatctt cgtcactgca ctggtgagta ttctctacta catcggtgtg atggggattt
     1201 taattcgcat tctcggcggt atattccaga aagcattaaa tatcagcaag attgagtcat
     1261 tcgtcgcggt caccaccatt ttcctcgggc aaaacgaaat tccggcgatc gtcaaaccct
     1321 ttatcgatca tctgaatcgc aatgaattat ttacagcgat ttgtagtggc atggcctcga
     1381 ttgctggttc gacaatgatt ggttacgccg ccctgggcgt acctgtggaa tatttgctgg
     1441 cggcatcgtt aatggcgatc ccaggcggga tcttgtttgc tcgcctgtta agcccggcta
     1501 cggaatcttc gcaggtttcc tttaataacc tctctttcac cgaaacaccc caaaaagcat
     1561 tattgaagcc gctgcgacag gggcaatgac cgggctgaaa atcgccgccg gtgtagcgac
     1621 agttgttatg gcatttgtcg ccatcattgc gttaattaat ggtattatcg gcggcgttgg
     1681 cggctggttt ggttttgcac atgcctcgct ggagtccatt ttaggttacc tgttggcccc
     1741 attggcgtgg gtgatggggg ttgactggag tgatgcaaat cttgccggga gtttgattgg
     1801 gcagaagctg gcgatcaatg aatttgtcgc ttatctcaat ttctcgccat atctgcaaac
     1861 gggtggcact ctggatgcta aaaccgtggc gattatttct ttcgcgttgt gcggtttcgc
     1921 taactttggt tctatcgggg tggtggtggg ggcgttttct gcggttgcgc cacaccgtgc
     1981 gccggaaatc gcccaacttg gtttacgcgc gctggcggcg gcgacacttt ctaacctgat
     2041 gagtgctgct attgcaggat tctttattgg tttagcgtag tgcgcaattt atgagccgga
     2101 taagatgcgg tcaagcgtcg aatccggcaa cattgttaca ccattggcac taatgaaagc
     2161 gcgttatcgg cagacagggt agggttgttt aagctgccgc tggcgcgcga aattgccgcg
     2221 caggctaccg caaaacgggc gctgtcgcgg aaactgtatc cttcaagaaa gctatatacc
     2281 aggcccgcca taaaaccatc gccagcacca aaactgtcta ctgtcgtatg cgctggcgcg
     2341 gtcagcaaaa attgttctcc atccttttcg ctgcaataaa ctgactcatc gggcaaataa
     2401 acaaacagtt gctgaacacc ttgctgatgc aatgcattca ctgcggcatt acggtcagca
     2461 tcgctggtga tcgcctgtcc ccataaaatt tccagctccg gtaaagtggg tttcagggtg
     2521 tgaatatgcg ccagccagtg tttgattttg cccgctttga attctgaaac ggtatcgaca
     2581 aacaccggga tttcatcagc gagggtaaag acccattcca gcgcctcggc tgtcaggttg
     2641 caatcagcca gtaccacgcc cgcatgacga agtaaatcgc gcgacccgtt cagtagttgc
     2701 ggtgtcaact gttccagcag atgggtatcg ttaatcgcca gcactgtttg atcgtctcga
     2761 ttggcaattg ccagatacgt cgatgtgctt tgaccatgca aacgaacgca gccggagaca
     2821 ttcacgccgg cacggcgcgt ttcttccagg agcatttcgc cataaaagtc atcgccaatc
     2881 actgaaagca aatggacgtc acggcctaac agcgccagat tgtgggcgat gttgcgtccc
     2941 acgccgcccg ccgagcaatg aattgtaccg ggatgagaag ccgcttgcgg gtaacggata
     3001 tccgcgatcc cgcgaatatc catattgatt gtccccacca ctacgcagta ttcctgctcg
     3061 gtgagaatgt aacctttgcc tttaatccgc cctttgcgca ttaaatccat aatatgcgcc
     3121 gcaacgcgcg aacgactgat ttgcagcatg tccgcaattt cgttctgctg aatcagcggg
     3181 ttacgccgta aaattgcaag gatctccttt tcccggttat tcatcttcag gctaccgctt
     3241 tttcagtttg ttgcgctttc agccaggcga tctcttctgc ccagatatcc gggttgatgg
     3301 tttcaaggat cagcggaatg ccgtcgaaac ggtcgtcctg catgatccag cggaacgcat
     3361 catgaccgat attgccttca ccgaggctat gatggcggtc aacgcggctg ccaaaggtgc
     3421 ttttcgcatc gttaaggtgc atcccgcgca gatacttaaa gccaacaata cgggcaaaat
     3481 ccgcgaacgt tttctcgcat tcggctggag tacgcaaatc atacccggca gcgaaagcat
     3541 ggcaggtatc aatgcagacg ccgacacggg atttatcttc cacgccgtcg ataatcgccg
     3601 cgagatgttc gaatttaaac cctaagttac tgccctgacc ggcggtgttt tcaatcaccg
     3661 ctgttacacc ttgagtttta tccagcgcaa tgttgatgga ttcggcaata cgcgcaaggc
     3721 aatcctcttc tgaaatctgc atcagatggc tgccagggtg gaagttgagc aaagaaagcc
     3781 ccagttgttc gcaacgctgc atttcatcta taaaggcatc gcgcgatttt tccagagctt
     3841 cagtgaccgg atgtccgagg ttaatcagat agctgtcgtg gggaagaatt tgcgccgatg
     3901 tgtagtgata tttttcacag gctgctttga attcatcgat ggtttgcgtc gtgagcggtg
     3961 cggcacgcca ctgacgttgg tttttggtga acaaggcaaa cgcggttgcg tcgatttcgg
     4021 cggcgcgaat tgcggcattt gccagaccgc cagcagcact aacgtgcgct ccaatgtatt
     4081 tcatgcgagg actcctgtta aacccgctgg aggaaaacgg taatgatagc gggttaacag
     4141 gagcaagatg tagtggttta tgcgatgacg ctttgaatca catagttaat cgcaccacca
     4201 ccaacaatca gccaggcaaa cagtaccagt gccatcagca gaggtttcgc cccggctttt
     4261 ttcagcgcgc tgacgtgagt ggtcagaccc agcgccgcca tcgccattgc cagcaggaag
     4321 gtatccagcg ttaccagcat gttcaccacg ctctgcggta acaggtggaa cgagttaaag
     4381 atggcaacga tgatgaacaa gatggcaaac cacggaatag tgattttgct tttctcgccg
     4441 ctgttcgccc cagacagctg tttaacacgc gccgccagca ggatgaggaa cggagccagc
     4501 atcatcacgc gcagcatttt ggaaataact gctgcgtttt ccgcatccgg gctgatggca
     4561 tgacctgccg ccaccacctg cgccacttcg tgcacagtag aaccaatgta gataccgaaa
     4621 gtttccggac taaaccattg agacatcagc ggatatatcg ccgggtagag gaaaatcgcg
     4681 acggtcccga agataacaac ggttgcaaca gccacggtta ctttactggc ttccgctttc
     4741 actaccggct cagtcgccag taccgcggca gcaccacaga tactgctacc ggcaccgatc
     4801 aaccagctgg tgtgcttatc cagaccaaac actttctgcc ccaggaagca agccagcagg
     4861 aaggtactgg acagcgtcaa cacgtcaatg atgatcccac tgataccgac atcggcaatt
     4921 tgcgagaacg tcagacggaa gccataaaga atgataccca gacgtaataa atattgcttg
     4981 gcaaacagca caccaccgtc acagcttttc cagatgtgcg gatagatggt gttgcctaaa
     5041 accatcccca acaagattgc gagggtgagg gcactaaacc cggcacccgc aaccgcggga
     5101 atggaaccac cccacagggc gaccccggtg ataactgcac tcagggctaa ccccggaata
     5161 aaatgccaca gtgtacgatg ttgtttctgt aaggtgatat tcgtcataac cctctcctta
     5221 taaccgggca taaggttacg actaactggt ttaaaaataa aattgattat atatttataa
     5281 ttaatcttta taagtggtaa gcgactatgc acatcaccct ccggcagttg gaagtttttg
     5341 cagaagtatt gaaaagtgga tcaaccaccc aggcgtcggt gatgctggcg ttgtcgcaat
     5401 cagcagtgag cgcagccttg accgacctgg aagggcagct tggcgtgcaa ctgtttgatc
     5461 gcgtggggaa aagactggtt gttaatgaac acgggcggct gctctatccg cgtgcgttgg
     5521 cattgcttga acaggcggtt gaaatcgaac aactgtttcg cgaagacaac ggcgcgattc
     5581 gtatctatgc cagtagtacc atcggtaact acattctgcc tgcagttatc gcccgttatc
     5641 gccatgatta tccgcagttg ccgattgaac ttagcgttgg gaacagccag gacgtgatgc
     5701 aagcggtgct ggatttccgc gttgatattg gctttattga aggaccgtgc cacagcactg
     5761 aaatcatttc tgaaccgtgg ctggaagacg agctggtggt tttcgccgcg ccgacttcgc
     5821 cgttggctcg tggtccggtc accttagaac agctggccgc tgcgccgtgg atcctgcgtg
     5881 aacgcggttc cggcacgcgg gagattgtcg attatctatt gctgtcacat ttaccgaagt
     5941 ttgagatggc gatggaatta ggtaactccg aggcaatcaa acatgcggtg cgtcatgggt
     6001 tgggaattag ttgcctgtcg cgacgggtga ttgaagatca attgcaggca ggcacattaa
     6061 gtgaagttgc ggtccctctg ccgcgcctga tgcgtacgtt gtggcgtata catcatcggc
     6121 aaaaacacct ttccaacgcg ctacggcgct ttctggacta ttgcgatccc gcgaatatac
     6181 cgcgttaagt tgctgtacaa gaacatgctg gtgctgtgtc gatttcgtga cgcagcgccc
     6241 tcagcatgca ttcgccagaa aagagattgg ctgctttact tataatccca gggcgatcat
     6301 gaaggtgtct tataaccgtg tatttctgcc ggaaggattg ccaatcgtct gctacaatcg
     6361 cgcctcattt ttaagatgga tagcattttt gtatggtttc cgaaactaaa accacagaag
     6421 cgccgggctt acgccgtgaa ttaaaggcgc gtcacctgac gatgattgcc attggcggtt
     6481 ccatcggtac aggtcttttt gttgcctctg gcgcaacgat ttctcaggca ggtccgggcg
     6541 gggcattgct ctcgtatatg ctgattggcc tgatggttta cttcctgatg accagtctcg
     6601 gtgaactggc tgcatatatg ccggtttccg gttcgtttgc cacttacggt cagaactatg
     6661 ttgaagaagg ctttggcttc gcgctgggct ggaactactg gtacaactgg gcggtgacta
     6721 tcgccgttga cctggttgca gctcagctgg tcatgagctg gtggttcccg gatacaccgg
     6781 gctggatctg gagtgcgttg ttcctcggcg ttatcttcct gctgaactac atctcagttc
     6841 gtggctttgg tgaagcggaa tactggttct cactgatcaa agtcacgaca gttattgtct
     6901 ttatcatcgt tggcgtgctg atgattatcg gtatcttcaa aggcgcgcag cctgcgggct
     6961 ggagcaactg gacaatcggc gaagcgccgt ttgctggtgg ttttgcggcg atgatcggcg
     7021 tagctatgat tgtcggcttc tctttccagg gaaccgagct gatcggtatt gctgcaggcg
     7081 agtccgaaga tccggcgaaa aacattccac gcgcggtacg tcaggtgttc tggcgaatcc
     7141 tgttgttcta tgtgttcgcg atcctgatta tcagcctgat tattccgtac accgatccga
     7201 gcctgctgcg taacgatgtt aaagacatca gcgttagtcc gttcaccctg gtgttccagc
     7261 acgcgggtct gctctctgcg gcggcggtga tgaacgcagt tattctgacg gcggtgctgt
     7321 cagccggtaa ctccggtatg tatgcgtcta ctcgtatgct gtacaccctg gcgtgtgacg
     7381 gtaaagcgcc gcgcattttc gctaaactgt cgcgtggtgg cgtgccgcgt aatgcgctgt
     7441 atgcgacgac ggtgattgcc ggtctgtgct tcctgacctc catgtttggc aaccagacgg
     7501 tatacctgtg gctgctgaac acctccggga tgacgggttt tatcgcctgg ctggggattg
     7561 ccattagcca ctatcgcttc cgtcgcggtt acgtattgca gggacacgac attaacgatc
     7621 tgccgtaccg ttcaggtttc ttcccactgg ggccgatctt cgcattcatt ctgtgtctga
     7681 ttatcacttt gggccagaac tacgaagcgt tcctgaaaga taccattgac tggggcggcg
     7741 tagcggcaac gtatattggt atcccgctgt tcctgattat ttggttcggc tacaagctga
     7801 ttaaaggaac tcacttcgta cgctacagcg aaatgaagtt cccgcagaac gataagaaat
     7861 aagtttcctc ccttccttgc taagccctct caaccgagag ggctttttca attccatttc
     7921 cctgacaaat catgcggata taaaatttaa catttggatt gataattgtt atcgtttgca
     7981 ttatcgttac gccgcaatca aaaaaggctg acaaatcaga ggctgttccg gctttctggg
     8041 atgatcacct gcatacaaat aagtccaccg cgatgctgcc gtacgcaagg ggacgtgaag
     8101 aaggtgtgag cgataaccca ttttattttc gtagttacct cattgagata tggaatgttt
     8161 aggttgaacc ctttcgtacg ggtcaggctg tgtttgtccg ctatttcttg tgcatggcct
     8221 gtgttagcgg tcgatgatga tggcgaaacg atggttgtca ctgcatcttc cgttgaacaa
     8281 aacctcaaag atgcacccgc cagtatcagc gtcattaccc aggaagacct gcagcgaaaa
     8341 ccggtacaga atctgaagga tgtcctcaaa gaagtgcctg gcgtacaact gacgaacgaa
     8401 ggggataacc gtaagggcgt tagtattcgt ggtctggaca gcagctacac cctgattctc
     8461 gtcgacggta aacgcgttaa ctcccgcaat gccgtcttcc gccacaatga tttcgatctg
     8521 aactggatcc cggtcgattc catcgaacgt attgaagtgg ttcgtggccc gatgtcgtcg
     8581 ctatacggct ccgatgcgct cggcggtgta gtgaatatca tcaccaaaaa aatcggtcag
     8641 aaatggtcgg gtaccgttac cgtcgatacc accattcagg aacatcgcga tcgcggtgat
     8701 acctataacg gtcagttctt taccagtgga ccattaattg atggtgtgct gggaatgaaa
     8761 gcttacggca gcctggcaaa acgtgaaaag gatgacccgc aaaactcaac gaccaccgat
     8821 accggagaaa tgccgcgtat tgaaggattc tccagccgcg acggcaatgt cgaatttgcc
     8881 tggacaccga atcaaaatca cgattttact gccggatacg gtttcgaccg tcaggaactc
     8941 gctggacaaa aaccgcctgg aacgccagaa ctactccgtc agccataatg ggcgttggga
     9001 ttacggcacc agcgaactga aatactacgg tgagaaagtc gagaacaaaa accctggcaa
     9061 cagcagcccg ataacttccg aaagcaatac ggtcgacggc aaatacacgt tgccgctgac
     9121 ggcgattaat cagtttctca cggttggcgg tgaatggcgt cacgacaaac ttagcgatgc
     9181 ggtgaacctg accgggggaa ccagctccaa aacgtctgcc agccagtacg cgctgtttgt
     9241 ggaagatgaa tggcggatct tcgagccgct ggcgctgacg accggcgtgc gtatggacga
     9301 tcacgaaacc tacggtgaac actggagtcc gcgtgcctac ctggtttata acgccaccga
     9361 caccgtaacg gtgaaagggg gctgggcgac ggcatttaaa gcaccttctc tgttgcaact
     9421 tagccctgac tggacgagca attcctgccg tggcgcatgt aagattgtgg gtagcccgga
     9481 tctgaaacca gaaaccagcg aaagttggga gctggggctt tactacatgg gtgaagaagg
     9541 ctggctggaa gggggggaat ccagcgttac cgttttccgt aacgatgtga aagatcgtat
     9601 cagcatcagc cgtacgtctg acgtcaacgc tgcaccgggc taccaaaact ttgttggttt
     9661 tgagacgggc gctaacggac ggcgtatacc ggtatttagc tactacaacg ttaacaaagc
     9721 tcgtattcag ggcgtggaaa ccgaactgaa aattccgttc aacgatgaat ggaaactgtc
     9781 gatcaactac acctacaacg atggtcgtga tgtcagcaac ggcgaaaaca aaccgctatc
     9841 cgatctgccg ttccatactg ctaacggtac gctggactgg aaaccgctgg cgctggaaga
     9901 ctggtcattc tatgtttctg gtcactatac cgggcagaaa cgcgccgaca gcgcgacggc
     9961 taaaacaccg ggcggttata ccatctggaa taccggcgcg gcctggcagg tgactaaaga
    10021 cgtcaaactg cgcgcaggcg tgctgaacct tggcgacaag gatctcagtc gtgacgacta
    10081 cagctataac gaagacggac gtcgttactt tatggcagtg gattatcgct tctgatgaga
    10141 agatgcccgg cgaaccgggc ggactttcac ttcagtaaat actgcgcatg gaagcgcagg
    10201 tgatcctcta taaaagaggc gatgaagtag taactgtgat catatcccgg ctgaatacgc
    10261 agcgtcatcg gccacgcttt ctggcgcgcg gcttctgcca gtaccgcagg ttgcaactgg
    10321 tcggcaagaa actgatcatt atcgccctga tcgataagcg tcgggatcgc atcctgcgcg
    10381 ttactggcat acatcagcgc gcaactgtcc cattccagcc atgcattttt gtcctcacct
    10441 aaatagctgc taaacgcttt gattccccac gggacgctgc acggattcac aattggcgca
    10501 aaggccgaaa cgctggtgta tttacccgga tttttcagcg ccataatcag cgcaccgtga
    10561 ccacccattg agtgaccgct aatggcgcag cggtcgctga cattaaattg cgactgaacc
    10621 agcgccggta attcatcgcg cagataatca tacatccggt aatgcgtcgc ccacggcggt
    10681 tgcgtggcat taagataaaa gcctgcgccc tggcctaaat cgtagccatc gtcgttggca
    10741 accttttcgc cgcgcgggct ggtgtctggc atcaccagta caatccccag ttctgccgcc
    10801 acgcgctggg cacccgcctt ggtggtgaag ttctcgtcat tgcaggttaa tccggaaagc
    10861 cagtacagca ctggtggcgg ggtgtgatca cgagggggtg ggagaaagat actgaacgtc
    10921 atcgggcagt ttaaggtact ggagtcgtgt cgccagcgtt gctgccagcc ttcaaaacag
    10981 cggtgctctt cgagcatttc catgcgtggc tccttgttgt gttgtttgca atttcttcat
    11041 cataccgttt attcaatgtg ctgtgagtaa ctttcacttc cgtatttgca taacgatgtt
    11101 ttaacatctg ctgatgaaag gcagcggcaa ttacaataat tatcgctgtg aatactggat
    11161 tatgtgcgcc gcctcacgca caataatcag gctgtaaatc agcttaataa ctttgccccc
    11221 acgcagggcg gaggcgtcac acctgcagga gaaatcataa atgccatcac tcagtaaaga
    11281 agcggccctg gttcatgaag cgttagttgc gcgaggactg gaaacaccgc tgcgcccgcc
    11341 cgtgcatgaa atggataacg aaacgcgcaa aagccttatt gctggtcata tgaccgaaat
    11401 catgcagctg ctgaatctcg acctggctga tgacagtttg atggaaacgc cgcatcgcat
    11461 cgctaaaatg tatgtcgatg aaattttctc cggtctggat tacgccaact tcccgaaaat
    11521 caccctcatt gaaaacaaaa tgaaggtcga tgaaatggtc accgtgcgcg atatcactct
    11581 gaccagtacc tgtgaacacc attttgttac catcgatggc aaagcgacgg tggcttatat
    11641 cccgaaagat tcggtgatcg gtctgtcaaa aattaaccgt atcgtgcagt tctttgccca
    11701 gcgtccgcag gtgcaggaac gtctgacgca gcaaattctt attgcgctac aaacgctgct
    11761 gggcaccaat aacgtggctg tctccattga tgcggtgcat tactgcgtga aagcgcgtgg
    11821 catccgcgat gcaaccagtg ccacaacaac gacctctctt ggtggattgt tcaaatccag
    11881 tcagaatacg cgccacgagt ttctgcgcgc tgtgcgtcat cacaactgat taaaaggcag
    11941 gaaccatgga gcgcaacgta acgctcgatt ttgttcgcgg cgtcgccatt ctggggatcc
    12001 tgctattaaa catcagcgcc tttgggctac caaaggcggc ttatctcaat cccgcctggt
    12061 acggcgctat tacgccgcag gatgcatgga cctgggcatt tctcgatctc atcggccagg
    12121 tgaaattcct cacgcttttt gcgctgctgt ttggtgcggg cctgcaaatg ttgctgcccc
    12181 gtggcagacg ctggatccag tcgcggttaa cgctgttagt cttgctgggt tttattcacg
    12241 gtttattgtt ctgggacggc gatattctgc tggcttacgg gctggtgggc ttaatctgct
    12301 ggcggctggt gcgcgatgcg ccatcggtaa aaagcctgtt taatacaggc gtcatgcttt
    12361 atctggtggg gcttggcgtt ttgctgttat tggggctgat ttccgacagc cagaccagcc
    12421 gcgcctggac gccggatgca tcggctattt tgtatgaaaa atactggaag cttcacggcg
    12481 gcgttgaagc gatcagtaat cgtgccgatg gtgttggcaa cagtttactg gcactgggcg
    12541 cacagtatgg ctggcaactg gcagggatga tgctcattgg tgcggcgctg atgcgcagcg
    12601 gctggctgaa agggcagttc agcttacgtc actatcgtcg tactggtttt gtgctggtgg
    12661 cgattggggt gaccattaac cttcctgcca tcgccctgca atggcagctg gactgggcgt
    12721 atcgctggtg tgccttctta cttcaaatgc cgcgggaact gagtgcgccg tttcaggcga
    12781 ttggctatgc gtcgctgttt tatggcttct ggccgcaatt gagccgcttt aagctggtgc
    12841 ttgcgatcgc ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa acgctgattt
    12901 gtaccatgct tttttaccac ctcggtttgt ttatgcattt tgaccgcctg gagctgctgg
    12961 cgtttgttat tccggtatgg ctggcgaata tcctcttctc tgttatctgg ctgcgttact
    13021 tccgccaggg gccggtggaa tggctctggc gtcagttaac tttgcgtgct gccggaacgg
    13081 caatatctaa aacatcaaga taacgatctg gatcacattc gttaacaaaa cggctgtaac
    13141 cgtttccatt gctgtgactc gattcacgaa gtcctgtatt cagtgctgac aaaatagccg
    13201 ccagcaagca gtcatttact gcaatctcat aacaggtagt gaatatgatc accattcgtg
    13261 atgtagcgcg tcaggctggc gtctctgtgg caacggtttc ccgggtgctc aataacagca
    13321 cgctggtcag tgccgacacg cgtgaagcag taatgaaagc cgtgagtgag ctggattatc
    13381 ggccaaacgc caatgcccag gcgctggcaa ctcaggttag cgacaccatt ggcgtggtgg
    13441 tgatggacgt ttctgatgcg tttttcggcg cgctggtaaa agcggtggat ctagtcgctc
    13501 agcagcatca gaaatacgtg ctaatcggca atagctatca tgaagcggaa aaagagcgtc
    13561 acgccattga ggtgttaatt cgccagcgtt gtaatgcgtt gattgttcac tcaaaagcat
    13621 tgagtgacga tgaactggcg caatttatgg ataacattcc cggtatggtg ttaatcaacc
    13681 gcgttgtgcc ggggtacgcc catcgttgcg tttgcctgga taatctcagc ggtgcccgaa
    13741 tggcgacgcg catgttgctg aataacggtc atcaacgtat tggttatctt tcttccagtc
    13801 acggcattga agatgacgcc atgcgtaaag caggctggat gagtgcgttg aaagagcagg
    13861 atattattcc gccggaaagc tggattggca ctggtacgcc ggacatgccg ggcggtgagg
    13921 cggcgatggt tgaactgctg gggcgcaatc tacaacttac cgctatattt gcttataacg
    13981 acaatatggc cgctggcgca ctgacagcat taaaagataa tggcattgcg attccgttac
    14041 atctctcaat catcggtttc gatgatattc ccatcgcccg ttacaccgac ccgcaattaa
    14101 cgaccgtgcg ttatcccatt gcttcaatgg ctaaattagc caccgaactg gccttgcagg
    14161 gggcagcagg caatattgat cctcgtgcca gccactgttt tatgccgacg ttagtgcgtc
    14221 gccattctgt cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca aatcaggcga
    14281 tgtaaccgct ttcaatctgt gagtgatttc acagaatctt aacaatgtga tagctatgat
    14341 tgcaccgttt taacgttgta acccgtatgt aacagtgaat aatcactttc accgaggtaa
    14401 tagcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg gaagaacgaa
    14461 ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc cctgccgagc
    14521 atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat aagaaggtgt
    14581 taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cgctgcacac gctgctgata
    14641 ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta gtgcgcaagg
    14701 ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat gattctcaga
    14761 atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg gtgaaggcac
    14821 tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa gcgcgtgggc
    14881 aaaacgtgcc ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg gatagctacg
    14941 acaaagccta ctacgttggc actgactcca aagagtccgg cattattcag ggcgatttga
    15001 ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa caaagacggt cagattcagt
    15061 ccgtactgct gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc acttacgtga
    15121 ttaaagaatt gaacgataaa ggcatcaaaa ctgaacagtt acagttagat accgcaatgt
    15181 gggacaccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg aacgccaaca
    15241 aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta gaagcactga
    15301 aagcacacaa caagtccagc attccggtgt ttggcgtcga tgcgctgcca gaagcgctgg
    15361 cgctggtgaa atccggtgca ctggcgggca cagtactgaa cgatgctaac aaccaggcga
    15421 aagcgacctt tgatctggcg aaaaacctgg ccgatggcaa aggtgcggct gatggcacca
    15481 actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat aaagacaacc
    15541 tggctgaatt cagcaagaaa taagactgat tgggcgcagt cgtttactgc gcccttattg
    15601 tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc ggggaatact
    15661 tgttggaaat gagcggtatc aacaagtcct ttcctggtgt taaggcactt gataacgtta
    15721 atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggt gcaggaaaat
    15781 cgacattatt aaaatgcctg tttggtattt atcaaaaaga ctccggcacc attttattcc
    15841 agggtaaaga gatcgatttc cattccgcca aagaggcact ggaaaatggt atttcgatgg
    15901 tacaccagga gttaaacctg gtattacaac gttcggtgat ggataacatg tggctggggc
    15961 gatatcccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa accaaagcga
    16021 tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca ttatccgttt
    16081 cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa attgtgatta
    16141 tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc actattattc
    16201 gtaaattaaa agagcgcggc tgcggtattg tttatatctc gcataaaatg gaagaaatct
    16261 tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc accgaaccgc
    16321 tggcaggact gacgatggac aagatcatcg ccatgatggt tgggcgttct cttaatcagc
    16381 gttttcctga caaagaaaac aagccgggcg aagtcatcct cgaggtgcgt aacctaacgt
    16441 cactgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg gagatcctcg
    16501 gtattgccgg gctggtgggg gcgaaacgta ccgatattgt tgagacgtta tttggtattc
    16561 gcgagaaatc ggctggcacc attacgttgc acggcaaaca gatcaataac cataatgcca
    16621 acgaagccat aaaccacgga tttgcactgg taactgagga gcgccgctca acgggaattt
    16681 atgcctatct ggatattggt tttaactcgt taatttccaa tattcgcaac tacaaaaata
    16741 aagttggttt actggataaa tcgcggatga aaagcgatac ccagtgggtg attgattcga
    16801 tgcgggtaaa aacaccgggg catcggacgc aaattggttc gctctcaggt ggtaatcagc
    16861 aaaaggtgat tattgggcgc tggctactaa cgcaaccaga aatattaatg ctcgatgaac
    16921 cgacgcgcgg tattgatgtc ggggcgaagt ttgagatcta tcagttaatt gccgaactgg
    16981 cgaagaaagg caaagggatt attattatct cctctgaaat gcctgagttg ttagggataa
    17041 cagaccgtat tctggtcatg agcaatggtc tcgtttccgg aattgtcgac acaaaaacaa
    17101 caacgcaaaa cgaaattctg cgtcttgcgt ctttgcacct ttaagattag gggcttccca
    17161 tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt atttacgtcg
    17221 ttcttttagt tttgctggcg attattattt tccaggaccc aacattttta agtctgttga
    17281 acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctc ggtgtggcag
    17341 ggttaattgt cacccagggg accgaccttt ctgcggggcg tcaagtaggg ctggcagcag
    17401 tggtggcagc gacattattg cagtccatgg ataacgccaa taaagtgttc ccggaaatgg
    17461 cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg gtgatcggtt
    17521 tgatcaacgg tctgattatc gcttatctca acgttacgcc gttcattacc acgctcggca
    17581 cgatgatcat cgtctatggc atcaactcgc tctattacga ctttgtcggg gcgtcgccaa
    17641 tttctggttt tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg ctggggagtt
    17701 tccgtctctc ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc tgggtgttgt
    17761 ggaacaaaac ccgcttcggt aagaacattt ttgccattgg cggtaacccg gaagcggcaa
    17821 aagtatctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg tctggcgtgt
    17881 tctatgcctt tggcgggatg ttagaagccg gacgtatcgg ctctgccacc aacaacctcg
    17941 gctttatgta tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta tcgttcagcg
    18001 gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc gtcatcaact
    18061 atggcctgac gtatatcggc gtaaacccat gctggcagta catcatcaaa ggggcgatta
    18121 ttatcttcgc cgtagcgctg gattcactga aatacgcgcg taagaaatga tgtgaatgtg
    18181 gtccgcattc ggtcataaac gcacataaca tcatgatctg tgtggttttg taggccggat
    18241 aaggcgttca cgccgcatcc ggcaacagga gccaaatgcc ggatgcaacg ctggcgcgtt
    18301 ttatccggcc tacaataagc tcaacatact gatttgtatg gttttgtagg ccggataagg
    18361 cgttcaagcc gcatccggcg acaggaacca aatgccggat gtgacgcggg ttctacaacg
    18421 ttaccgggtg ttctttctcg cctttcttaa acttcacttc cccgagctca atacaaccca
    18481 ccgggcagac gtgaccacaa agcagacaac ccacacattt ctcggtatta caatgcggtg
    18541 tgcgggtttt ctcgctccat tccatcgcct ggtgaccgcc gtcgtaacag gaaatataac
    18601 agcgtccaca gccaacacat ttgtcaagat tgatacgggg atagacaata taactgcggt
    18661 ctaaatcttc cgccgggaca atattgttat tcgccagacc taccatctcc tgcaacgaat
    18721 caaatccctg atcggcgaga taatgcgata acccgctcgc catatcttct acgatgcgat
    18781 tgccgtactg cataatgccg gtggtgacct gtaacgttgc tgcgccgagc aataaaaact
    18841 ccgccgcatc ctcccaggtt tcaatgccgc cgatac
//
LOCUS       DAJTSL010000074        18742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTSL010000074 DAJTSL010000000
VERSION     DAJTSL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.074"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..893
                     /locus_tag="OO433_002274"
     CDS             <1..893
                     /locus_tag="OO433_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6050735.1"
                     /translation="WFNIKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFIT
                     IGVLGIFLAIGWYMLYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTM
                     WGMMLGFSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMTAIPFLFGAAGMLVNGYVT
                     DWLVKGGMAPIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTS
                     CRGLIHVAVASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTA
                     AGALAYIFLVRQPINDPRKD"
     gene            complement(942..2597)
                     /gene="tsr"
                     /locus_tag="OO433_002275"
     CDS             complement(942..2597)
                     /gene="tsr"
                     /locus_tag="OO433_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6050736.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2716..3162)
                     /gene="hpaR"
                     /locus_tag="OO433_002276"
     CDS             complement(2716..3162)
                     /gene="hpaR"
                     /locus_tag="OO433_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6050737.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3434..4723
                     /locus_tag="OO433_002277"
     CDS             3434..4723
                     /locus_tag="OO433_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6050738.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            4720..6186
                     /gene="hpaE"
                     /locus_tag="OO433_002278"
     CDS             4720..6186
                     /gene="hpaE"
                     /locus_tag="OO433_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6050739.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRDHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            6188..7039
                     /gene="hpaD"
                     /locus_tag="OO433_002279"
     CDS             6188..7039
                     /gene="hpaD"
                     /locus_tag="OO433_002279"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6050740.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            7049..7429
                     /locus_tag="OO433_002280"
     CDS             7049..7429
                     /locus_tag="OO433_002280"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6050741.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            7540..8343
                     /gene="hpaH"
                     /locus_tag="OO433_002281"
     CDS             7540..8343
                     /gene="hpaH"
                     /locus_tag="OO433_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6050742.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8354..9142
                     /gene="hpaI"
                     /locus_tag="OO433_002282"
     CDS             8354..9142
                     /gene="hpaI"
                     /locus_tag="OO433_002282"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6050743.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9317..10693
                     /gene="hpaX"
                     /locus_tag="OO433_002283"
     CDS             9317..10693
                     /gene="hpaX"
                     /locus_tag="OO433_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6050744.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            10703..11589
                     /gene="hpaA"
                     /locus_tag="OO433_002284"
                     /pseudo
     CDS             10703..11589
                     /gene="hpaA"
                     /locus_tag="OO433_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            11840..13402
                     /gene="hpaB"
                     /locus_tag="OO433_002285"
     CDS             11840..13402
                     /gene="hpaB"
                     /locus_tag="OO433_002285"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCR6050745.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSANDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQHIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13420..13932
                     /locus_tag="OO433_002286"
     CDS             13420..13932
                     /locus_tag="OO433_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6050746.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14325..16475
                     /gene="btsT"
                     /locus_tag="OO433_002287"
     CDS             14325..16475
                     /gene="btsT"
                     /locus_tag="OO433_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6050747.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16606..16809
                     /locus_tag="OO433_002288"
     CDS             16606..16809
                     /locus_tag="OO433_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6050748.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            16820..17776
                     /gene="yjiA"
                     /locus_tag="OO433_002289"
     CDS             16820..17776
                     /gene="yjiA"
                     /locus_tag="OO433_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6050749.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(17878..>18742)
                     /locus_tag="OO433_002290"
     CDS             complement(17878..>18742)
                     /locus_tag="OO433_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR6050750.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFDVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKES"
BASE COUNT         4288 a         5185 c         4993 g         4276 t
ORIGIN      
        1 actggttcaa catcaaagag cgcggacgcc cgatgggctt cttcaacgca gcttctacca
       61 ttggcgttgc cgtaagccca ccgattctgg cggcgatgat gctggtgatg ggctggcgcg
      121 ggatgtttat taccattggt gtactgggga tttttctcgc catcggctgg tatatgctct
      181 atcgcaaccg cgagcacgta gaactgactg ccgttgagca agcttatctc aatgcaggta
      241 gcgtcaatgc ccgccgagat ccgctcagtt ttgccgaatg gcgcagcctg ttccgtaacc
      301 gtacaatgtg gggaatgatg ctcggattca gtggcatcaa ctacactgcg tggctgtatc
      361 tggcctggct tcctggttac ctgcaaacag cctataacct ggatttaaaa agcacagggt
      421 tgatgacggc tatccctttc ctgtttgggg ctgccgggat gctggtcaac ggttacgtta
      481 ccgactggct ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc tgcattattg
      541 ccgggatgtt ctgttctgcc gcctttacgc tgatagtacc acaagcgaca acatccatga
      601 cggcggttct gctgattggc atggcactgt tctgtattca ctttgccgga acatcctgcc
      661 ggggcttgat ccacgtcgca gttgcttctc gcatgactgc gtcggtgggc agtatccaga
      721 actttgccag cttcatctgc gcctcttttg cgccgatcat tactggtttt attgttgata
      781 ccacccactc attccgtctg gcactaatca tctgcggttg cgtcaccgca gcgggggcac
      841 tggcgtacat cttcctggtt cgtcagccga tcaacgaccc acgtaaagat taatctcctt
      901 atgcccgata acattttgct tatcgggcat tttcatggcg attaaaatgt ttcccagttc
      961 tcctcgctat ctgccacggc cattttacgc ggcgcagctg gcgtcacggt ttttaccaca
     1021 gccgatgttt cacgctgctg ttgctgaatc cggaacactg ccactgcttc ggtcaggcga
     1081 ctggcctgct cttccagcgc ggcggcggca gcggcagact cttccaccag cgcggcgttc
     1141 tgttgagtta cccggtccat ctcagcaacc gctaagccaa cctgatcgat accacggctc
     1201 tgctcatcag aagcagaagc aatttcgccc ataatgtccg tcacgcgggt cacggcgctg
     1261 acaatctccg ccattgtttc cccggcgctt tcgaccagcg tagagccaac atccactttc
     1321 cccaccgagt cttcaatcag gcttttaatt tcacgagccg cctgggcgct gcgctgggcc
     1381 agattacgca cttctcccgc gaccaccgca aaaccgcgcc cttgctcacc cgcacgcgca
     1441 gcctcaaccg ccgcgttcaa agccagaata ttggtctgga aggcaatgcc gtcaattacg
     1501 ctgataatat cggcgatttt ctgcgaactg gtggagatat cgcgcatagt ctgcaccacg
     1561 ttatctacca ctttgccgcc gcgttgcgcc gtttcagaag cacttaacgc cagatggctg
     1621 gcctggcgcg cattttcggc gttctgcttc accgttgcgg tcagttgctc catgctggct
     1681 gccgtctctt ccagcgaagc ggcctgttgc tcggtgcgcg aagagagatc gttattgccg
     1741 gtggcgattt cgctggcacc gctatagatg gcattggccc cgttgcgcac atcaccgacg
     1801 gtacgcatca gctctccctg catatggcgc aaactctctg ccagttgccc catctcatta
     1861 gagccatcca cctcaatcgg tttcaccaga tcgccgcctg caatatgacg aatgctgtca
     1921 atcaggcgat tcattggcgc taccagcgag gctttaatac cgaaccagac ggcgaagatg
     1981 accgccagta cgacgatcat cacgcccacc agaatccaca tcgcctggct gtaggaggca
     2041 ttgttatcgc tgacggcgat atcatagagc cgatcgtttt gctccatgta agccacatac
     2101 tgcttctcga aaccgtcctg atatccctgg gtcggctgat caaagaactc gttgattttg
     2161 cctgcaccta acagttggat cagctccgcc agcgcattgt gataaatatc gtaattacgt
     2221 ttgatctctg ccgctgcggc ggtgctctga cgcgggtcac gcggcaacgc ttcgtaatcc
     2281 gcccagtttt tttccgcctg tttcagcgaa atactggcac tctgcatcag ctcagcaacg
     2341 gttgaaccgc taccaatatt attctgatcc atcatgtagc ggatacccgc gcggttgagg
     2401 gtgttacgcg tctgcaacaa cgcgacccag ctgccattca gcgtggattg ctgctggcga
     2461 atggtttgta aaacagtgaa attttctttg tcattcttta aggcattaaa gaacagaccg
     2521 cctgatgtca gttgtaaaag gccaaaaacg gccaaaacca gcagtaagct ggtcacgatc
     2581 ttgatacgtt ttaacatggt ttctctttcc tgtggaccga tgcaagattt tcggcctgga
     2641 aagcaaaaac tttatgaaaa ggtgtaaccc gccatccatg gaaaaaccag gcgctggtta
     2701 tcagatacta aaaagttatt cattatcgcc cgggatggct tcttgtcggg aattgcccag
     2761 agcaataaat tcctctaaca gatgggttaa ctgttgcatt ttctcggcag taaactgagc
     2821 ttcaatctgc cgataggctt cttcaatctg tgtctgagcg cggttataca gcgcctgccc
     2881 ctctttggtt agcgagatat agagcttccg ctgatcattg atcggcttta atcgcaacac
     2941 taaaccgtcg cgttccatcc gcgtgagaat cccggtcagg cttgggcgca aaatgcaggc
     3001 gcgatacgcc agatcgtgaa aatccattga tgggctttcc gccagaatac gcacgatgcg
     3061 ccactgttgc tcagtcaaat tatgccgttt aacgattggg cgaaaataac tcatcgccgc
     3121 ttctcgcgcc tggagcaacg caatggttag tgagtcgtgc attatctttc ccctgaaaaa
     3181 ttcaaaatca ttaatataga aacagttaat cgtcacgtta ccgggactgc gctgcccctg
     3241 caattcctga ttttatcaaa attgatgata ttttattaac ttactgatat ttaatatttt
     3301 attatcttta aatgtaaata gtttgttaat tagatcacat ttacatcact tacttttgcc
     3361 aaactacgca atcgggaata aaagcaatca ttaacatatt aatgattaag ataatcgaat
     3421 ccgaggagtg gtaatgaaag gcactatctt cgccgtagcg ttgaaccatc gcagccaact
     3481 tgatgcatgg caggaagcgt tccagcaatc cccctacaaa gccccgccta aaactgcggt
     3541 ctggtttatt aaaccgcgca atacggtgat tggttgcggt gaaccgattc cctttccaca
     3601 gggtgaaaag gtactgagcg gtgcgactgt tgcgctgatt gtgggaaaaa cggcgacgaa
     3661 agtacgtgaa gaagatgcgg cagagtacat cgccggatat gcgctggcta acgacgtcag
     3721 cctgccggaa gagagctttt accgcccggc aatcaaagca aaatgtcgtg atggattctg
     3781 ccccattggc gaaaccgtgg ctctcagcaa tgtcgataat ctgaccatct ataccgagat
     3841 caacgggcgt cctgccgatc actggaatac cgccgattta caacgtaacg ccgcgcagtt
     3901 gctgagcgcc ctgagcgaat ttgccacgct gaatccaggc gacgccattc tgctcggcac
     3961 gccacaggcg cgcgtggaaa tacagccagg cgatcgcgtt cgtgttctcg cagaaggttt
     4021 cccgccgctg gaaaatccgg tagtggacga acgtgaagtg accacgcgca agagcttccc
     4081 aacgcagcca cacccgcacg gcacgctgtt tgccctcggc ctgaactacg ccgaccacgc
     4141 cagcgaactg gaatttaagc caccggaaga accgctggta ttcctgaaag cgccaaatac
     4201 cctcactggc gataaccaga cctccgtgcg tccaaacaat attgaataca tgcactatga
     4261 agcggagctg gtggtagtta ttggcaaaca ggcgcgtaac gtcagcgaag ccgatgccat
     4321 ggattatgtc gcgggctaca ccgtgtgtaa cgactacgcc attcgtgact atctggaaaa
     4381 ctactaccgc cctaacctgc gggtaaaaag ccgcgacgga ctgacgccga tgctttcaac
     4441 catcgtgccg aaagaggcga tcccggaccc gcataatctg acccttcgca ccttcgtcaa
     4501 cggcgagtta cgccagcaag gcaccaccgc cgatctgatc ttcagcgtgc ccttcctgat
     4561 cgcctactta agtgaattta tgaccctgaa tccgggcgac atgatcgcca ccggcacacc
     4621 caaaggctta tctgacgtgg tgcctggcga tgaagtagtg gtggaagtag aaggcgtggg
     4681 ccgcctggtg aaccgaattg tgagtgagga tacagcgaaa tgaaaaaagt aaatcattgg
     4741 atcaacggca aaaatgttgc aggtaacgac tacttccaga ccaccaatcc ggcaacgggt
     4801 gaagtgctgg cggatgtggc ctctggcggt gaagcggaga tcaatcaggc ggtagcggca
     4861 gcgaaagagg cgttcccgaa atgggccaat ctgccgatga aagagcgtgc gcgcctgatg
     4921 cgccgtctgg gcgatctgat cgaccagaac gtgccagaga tcgccgcgat ggaaaccgcg
     4981 gacaccggcc tgccgatcca tcagaccaaa aatgtgttga tcccacgcgc ttcccacaac
     5041 tttgaatttt tcgcggaagt ctgccagcag atgaacggca agacctatcc ggttgacgac
     5101 aagatgctca actacacgct ggtgcagccg gtgggcgttt gtgcgctggt atcgccgtgg
     5161 aacgtaccgt ttatgaccgc cacatggaag gtcgcgccgt gtctggcgct gggcaatacc
     5221 gcggtactga aaatgtcgga actctccccg ctgaccgctg accgcctggg tgagctggcg
     5281 ctggaagccg gtattccggc aggcgtgctg aacgtggtac agggctacgg cgcaaccgca
     5341 ggggatgcgc tggttcgtga tcatgacgta cgtgccgtgt cgttcaccgg cggtacggcc
     5401 accgggcgca acatcatgaa aaacgccggg ctgaaaaaat actccatgga actgggcggt
     5461 aaatcgccgg tgctgatttt tgaagatgcc gatattgaac gcgcgctgga cgccgccctg
     5521 ttcaccatct tctcgatcaa cggcgaacgc tgcaccgccg gttcacgcat ctttattcag
     5581 caaagcatct acccggaatt cgttaaacgc tttgccgaac gcgccaaccg tctgcgcgtg
     5641 ggcgatccga acgatccgaa tacccaggtt ggcgcgctta tcagccagca gcactgggaa
     5701 aaagtctccg gctatatccg tctcggcatt gaagaaggcg ccaccctgct ggcgggcggc
     5761 ccggataaac cgtctgacct gcctgcacac ctgaaaggcg gcaacttcct gcgcccaacg
     5821 gtgctggcag acgttgataa ccgtatgcga gtcgcccaag aagagatttt cgggccggtc
     5881 gcctgcctgc tgccgtttaa agacgaagct gaaggcttac gcctggcaaa cgacgtggag
     5941 tacggcctcg cgtcgtacat ctggacacag gatgtcagca aagtgttacg cctggcgcgt
     6001 ggcattgaag ctggcatggt gttcgtcaac acccagaacg tgcgtgacct gcgccagcca
     6061 tttggcggcg taaaagcctc cggcaccggg cgtgaaggcg gtgagtacag cttcgaagtg
     6121 ttcgcggaaa tgaagaacgt ctgcatttcc atgggcgacc atccaattcc gaaatgggga
     6181 gtctgatatg ggtaagttag cgttagccgc caagatcacg cacgtaccgt cgatgtatct
     6241 ctctgaactg ccagggaaaa accacggttg ccgccagggc gcgatcgacg ggcataaaga
     6301 gatcagcaag cgttgccggg aaatgggcgt cgataccatt atcgttttcg atacccactg
     6361 gctggtcaac agtgcttatc acatcaactg tgcagaccat tttgaaggcg tctacaccag
     6421 caacgagctg ccgcatttta tccgcgatat gacctacaac tacgagggca acccggagtt
     6481 ggggcagctt attgccgatg aagccttaaa gctcggcgtg cgggcaaaag cgcacaacat
     6541 tcccagcctg aaactggaat acggcacgct ggtgccgatg cgctacatga atgaagacaa
     6601 gcacttcaaa gtggtctcca tttcggcttt ctgcacggtt cacgattttg ccgacagccg
     6661 caagctgggc gaagcgattc tgaaagcgat cgaacagtac gacggcaccg tggcggtcct
     6721 tgccagcggt tcgttatcgc accgctttat tgacgatcag cgtgcagaag aagggatgaa
     6781 cagctacacc cgcgagttcg accgccagat ggacgagcgc gtggtgaagt tgtggcgcga
     6841 aggccagttc aaagagttct gcaatatgct gccggagtac gccgactact gctacggcga
     6901 aggcaatatg cacgacacgg tgatgctgct ggggatgctc ggctgggata aatacgacgg
     6961 caaggtggag tttatcaccg agctattccc aagctctggc accggtcagg ttaacgctgt
     7021 tttcccgctt cccgcgtaag gacgttttat gccgcacttt atcgttgaat gcagtgacaa
     7081 catccgcgaa gaagccgacc tgccggggtt gttcgccaaa gtgaatccga cgctggcagc
     7141 cacgggtatt tttccgctgg cgggtattcg cagccgcgtg cattgggtcg atacctggca
     7201 gatggccgac gggcagcgtg attatgcctt cgtgcatatg acgttgaaaa tcggcgcagg
     7261 tcgcagcctg gaaagccgtc agcaggcggg tgaaatgctg tttgaactga ttaaaacgca
     7321 cttcgccgcc ctgatggaga gccgcctgct ggcgttgtcg tttgagattg aagagctgca
     7381 tccgacgctg aattttaaac aaaacaacgt gcacgcattg tttaagtagc gcgcagattg
     7441 cccggtggcg ctgcgcttac cgggcctaca aaaccccaaa ccgtacaccg taggccggat
     7501 aaggcgcagc cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac
     7561 accctgatcg cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc
     7621 tcgctggatt acccagagat caccatcgaa gacgcttacg cggtgcagcg cgaatgggtt
     7681 cgcctgaaaa tcgccgaagg ccgtacgctg aaaggccaca aaatcggtct gacctcaaaa
     7741 gcaatgcagg ccagctcgca gatcagcgaa ccagattacg gtgccctgct ggatgacatg
     7801 ttcttccacg atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg
     7861 gagctggctt tcgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtc
     7921 tacaacgcca cggactacgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac
     7981 atcgatccgg aaacccagcg cccgcgcaaa gtgttcgaca ccatttctga taacgccgcc
     8041 aacgccgggg tgatcctcgg tggtcgtccg atcaaaccag acgaactgga tctgcgttgg
     8101 atctccgccc tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggcgtg
     8161 ctgaatcatc cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta
     8221 caactggaag ccgggcaaat cattctcggt ggttcgttca cccgcccggt tccggcgcgt
     8281 aagggcgaca ccttccacgt cgattacggc aacatgggtt ccattagctg ccgctttgtt
     8341 taaggagaga acgatggaaa acagctttaa agcggcgctg aaagcaggcc gtccgcagat
     8401 cggattatgg ctggggctga gtagcagcta cagcgcagag ttactggccg gagcaggatt
     8461 cgactggttg ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca
     8521 gctacaggcg attgcgccct accccagcca gccggtagta cgtccgtcgt ggaacgatcc
     8581 ggtgcaaatc aaacaactgc tggacgtcgg cacacaaacc ttgctggtgc cgatggtaca
     8641 aaacgctgac gaagcccgtg aagcggtacg cgccacccgt tatccccccg ccggtattcg
     8701 cggtgtgggc agtgcgctgg cccgcgcctc gcgctggaat cgtattcctg attacctgca
     8761 aaaagccaac gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa
     8821 cttaccgcag attctggacg tggaaggcgt cgacggcgtg tttatcggcc cggcggatct
     8881 gagcgccgat atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga
     8941 gcaggcgatc gtgcagatcc gtgaatcggg caaagcgccg gggatcctga tcgccaatga
     9001 gcaactggca aaacgctatc tggaactggg cgcgctattt gtcgccgtcg gcgttgacac
     9061 caccctgctc gcccgcgccg ctgaagcgct ggcagcacgg tttggtgcgc aggccaccgc
     9121 cgtgaagccc ggcgtgtatt aatgccggat gcgctgcact tatccggcct acactcgcac
     9181 caaaccgtag gccggataag gcgtttacgc cgcatccggc aaaaagccgt accaaatcgc
     9241 ggaccggata aggcgttacg ccgcatccgc cataaacctt gtcgtaccct acaaaaatcc
     9301 cattagagga agaaaaatga gcgacacctc acctgccata ccggagagta tcgatccggc
     9361 gaatcagcat aaagcgctga ctgccggaca acaggcggtt attaagaagc tatttcgccg
     9421 cctgatcgtc tttctgttcg tgctgtttat cttctcgttc cttgatcgca tcaacatcgg
     9481 ctttgccgga ctcacgatgg gacgcgacct cggtctgagc gccaccatgt ttggcctcgc
     9541 taccaccctg ttctatgccg cttatgtcat cttcggcatt cccagcaaca ttatgctgag
     9601 tattgtcggt gcgcggcgct ggatcgccac catcatggtg ctctggggca tcgcctctac
     9661 tgccaccatg tttgccactg gccccaccag cttgtacgta ctgcgtatac tggttggcat
     9721 taccgaagcc ggctttctgc ctggtattct gctgtattta accttctggt ttccggccta
     9781 tttccgcgcc cgtgccaacg ccttgtttat ggtagcaatg ccggtaacga cagcgttggg
     9841 ctcgatcgtt tccggctaca ttttgtcgct ggatggcgtc atggcattaa aaggctggca
     9901 gtggctgttt ttgctggaag gcttcccgtc ggtattactc ggcgtcatgg tgtggttctg
     9961 gcttgatgac tcaccggaca aagctaagtg gctgacgaaa gaagacaaaa aatgcctgca
    10021 agagatgatg gataacgatc gtctgacgct ggttcagcca gagggagcca tcagccacca
    10081 cgccatgcaa caacgcagta tgtggcggga gatcttcact ccggtggtga tgatgtatac
    10141 cctggcgtat ttctgcctga ccaacacact tagtgcgatc agcatctgga caccgcagat
    10201 cctgcaaagt tttaatcagg gcagcagtaa tatcaccatc ggcctgctgg ccgccgtacc
    10261 gcagatttgt accattctcg ggatggtcta ctggagccgt cactcagatc gccgccagga
    10321 acgaaggcat cacaccgccc ttccttattt gttcgctgcc gcaggttggt tactggcttc
    10381 ggcaactgat cacaacatga tccagatgct ggggatcatt atggcttcga ccggatcatt
    10441 cagcgcaatg gcgattttct ggacaacacc ggatcagtcc atcagcctgc gggcacgagc
    10501 gatcggtatt gcggtgatca acgccactgg caacattggt tcagcgttaa gtccgtttat
    10561 gatcggctgg ttgaaagatt tgaccggcag ctttaacagt ggattgtggt ttgttgccgc
    10621 gctgctggtg attggtgcgg ggattatctg ggcgattcca atgcagtcct cccgtccgcg
    10681 agcgaccccg taaggaacga cgatgtgtga ccgtcagatt gccaatattg atatcagcaa
    10741 agagtacgat gaaagcctgg gcacggacga tgtgcattat cagtcgttcg cccgcatggc
    10801 ggcatttttt ggccgccata tgctgccaca tcgccacgaa cagtactttc agatgcattt
    10861 cctcaatagc ggacagattg agctacagct tgacgatcat cgctactcgg tggaagcgcc
    10921 cctgtttgtc ctgacgccgc cgtcagtacc tcatgcgttt attacggagt ctgacgccga
    10981 cggtcatgtg ttgacggtac gggaagatct gatctggccc ctgctggaag ttctttatcc
    11041 gggcactcgg gaaaccttcg gcctgccggg ggcctgtcac tggcagataa acccgacgaa
    11101 ctggcggcgc tggaacacta ttggcaactg atagagcggg aatcggtgga acaactgcct
    11161 ggacgggaac acaccctgac gttactggca caggcagtgt tcaccctact gctgcgtaac
    11221 gcaaaactcg acgaccatgc cgccagcggg atgcgcggag aattaaaact gttccagcgt
    11281 tttaatatgc tgattgaaag ccattttcat cagcactgga cagtaccgga ttacgctaac
    11341 gaactgcata tcaccgaatc acgactcacg gacatctgcc gtcgctttgc caaccgtccg
    11401 ccaaaacggt tgattttcga caggcagctg cgagaagcca agcggctgct gctgttttct
    11461 gataacgccg tgaacaatat tgcctggcaa ctcggtttta aggatccggc ttattttgcg
    11521 cgctttttta atcgcttagt cggttgctcg cccagtgctt atcgcgccaa aaaagtacct
    11581 gtgacgtgaa aatcccttag ccttaacggg aaaccaggca ccacctgcta ttcccctttc
    11641 tccgcctgga gcaaggggat ttagtaacgt caaaaatcgc taaaagcgac ctcgatcaca
    11701 aaaccgtacc cgacctgaaa agtaccagcc attgtcccaa aagtctcttc ccgtaatcgc
    11761 ccctgcaagc tttaattgaa taacaaaaac aacacacaaa attaacaatc gtattccgat
    11821 taatactgta gaggtcgaca tgaaaccaga agatttccgc gccagtaccc aacgtccgtt
    11881 caccggggaa gagtatctga aaagcctgca ggatggtcgc gagatctata tctatggcga
    11941 gcgagtgaaa gacgtcacta ctcatccggc atttcgtaat gcggcagcgt ctgttgccca
    12001 actgtacgac gcgctacaca aaccggagat gcaggactct ctgtgctgga acaccgacac
    12061 cggcagcggc ggctataccc ataaattctt ccgcgtggcg aaaagtgcca acgacctgcg
    12121 ccagcaacgc gatgccatcg ctgagtggtc acgcctgagc tatggctgga tgggccgtac
    12181 cccagactac aaagctgctt tcggttgcgc actgggcgcg aatccgggct tttacggtca
    12241 gttcgagcag aacgcccgta actggtacac ccgtattcag gaaactggcc tctactttaa
    12301 ccacgcgatt gttaacccac cgatcgatcg tcatttgccg accgataaag taaaagacgt
    12361 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    12421 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    12481 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtaaa
    12541 attaatctct cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta
    12601 cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata acgtgctgat
    12661 cccatgggaa aacgtactga tctaccgcga tttcgatcgc tgccgtcgct ggacaatgga
    12721 aggcggtttt gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaattaga
    12781 cttcattaca gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg
    12841 tgtacaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga
    12901 ctcgatgtgt tctgaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc
    12961 actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattatcga
    13021 acgcaacgtt accagtggcc tgatctatct cccttccagt gcccgtgacc tgaacaatcc
    13081 gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aatggtatgg atcacgtcca
    13141 gcacatcaag atcctcaaac tgatgtggga cgccattggc agcgagtttg gtggtcgcca
    13201 cgaactgtat gaaatcaact actccggtag ccaggatgag attcgcctgc agtgtctgcg
    13261 ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc
    13321 ggaatacgac cagaacggct ggactgtgcc gcacttgcac aacaacgacg atatcaacat
    13381 gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacagcgc
    13441 ctgcgctttc gtgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag
    13501 ggcgacgccg gacaatgcgg gattacggca acggccgtct gctcggtcac ggatacacca
    13561 ccatcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac
    13621 ggtaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcacg ccacttcgcg
    13681 ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg
    13741 ctggcgcagc cggtgctgaa aggttcgctg gccagtcttg aaggtgagat ccgcgatgtg
    13801 caggcaattg gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca
    13861 gaaggtcacg gacttatcta ctttaaacgc cgtttccatc cagtgatgct ggaaatggaa
    13921 gctgcgattt aagctccagc cccttcacgt attcgtcgaa ggggcatttt tattgtgatc
    13981 tcaatcacat atccacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc
    14041 gcttacgtcc ctaaaataac cactcagtta tttaccttac tttacgcgcg cgtaactctg
    14101 gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    14161 ggcttcggcc tatgattaac aaattactct atgaattttg gtttgcagga aggcagcaag
    14221 tgagtgaatc cccgggagct tacaacagta agtgacagag gtgaacgaac gcagctgccg
    14281 cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac
    14341 tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag
    14401 ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg
    14461 tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg
    14521 accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca
    14581 accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgccggt ccgctggttg
    14641 gtccggtgct cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg
    14701 tagtactggc cggtgcggtt caggacttta tggtgctgtt tatctcctct cgccgtaacg
    14761 gcgcatctct cggtgagatg atcaaagaag agatgggacc agtaccgggg actatcgcgc
    14821 tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta
    14881 aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc
    14941 tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtaggtgaa gtttctgtta
    15001 ttggtatcgt gctgctggtt gcctctatct atttcggtgg cgtgattgct cacgatccgt
    15061 actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg
    15121 cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa
    15181 ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggta ctgaacccgg
    15241 aactgaaaat gcctgcaatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg
    15301 ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc
    15361 tgatctcttc cggtacgacg ccgaaattgc tggctaacga aaccgacgcg cgtttcattg
    15421 gctacggcgc aatgctgatg gaatccttcg tggcgattat ggcgctggtt gctgcgtcca
    15481 tcatcgaacc gggtctttac ttcgcgatga ataccccgcc tgctggcctt ggcatcacca
    15541 tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag
    15601 acgttaccgc acacgcggcg gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc
    15661 agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggcg
    15721 cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca
    15781 tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg
    15841 atgcgggtac tcgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt
    15901 tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc
    15961 tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt
    16021 ggccgctgtt cggtatttcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg
    16081 ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat
    16141 ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga
    16201 tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc
    16261 tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag
    16321 gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct
    16381 ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aactccgtac gttccaatcc
    16441 cggaaggcgg cgtgaagatc tcttcgcacc actaaccggt tgccggatgc gctgcgcgta
    16501 tccggcctac aaagtgtcta tcgtaggccg gataaggcgt ttacgccgca tccggcaaat
    16561 ccctagcccg gaccttccgg gctttacgca tatctggaag gcaaaatgtt tggtaactta
    16621 ggacaggcaa aaaaatatct cggccaggcg gcgaagatgt tgattggcat tccagactac
    16681 gacaactacg tcgagcatat gaagaccaac catcccgaca agccgtacat gagctatgaa
    16741 gaattcttcc gcgaacgcca gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc
    16801 tgttgttaat ggagagagca tgaacccgat tgcagttacc ctactcaccg gttttttagg
    16861 cgcgggcaaa accaccctgc tgcgccatat tcttaacgaa caacacggct acaagattgc
    16921 cgtgattgaa aacgaattcg gcgaagtctc cgttgatgat caactgattg gcgaccgcgc
    16981 cacgcagatc aaaacgctga ccaacggctg catctgctgt tcgcgctcca acgagctgga
    17041 agacgcacta ctcgacctgc tggataatct cgacaagggc aatattcagt tcgaccgtct
    17101 ggtcattgaa tgcaccggca tggccgatcc cggcccgatt attcagacct tcttctccca
    17161 tgagatttta tgccagcgct acctgctgga cggggtgatt gcgctggtgg atgcggtaca
    17221 tgccgatgag cagatgaacc agttcaccat cgcccagtcc caggttggct acgccgaccg
    17281 tattctgctg acgaaaaccg acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc
    17341 gcgcatcaac gcccgcgcac cggtctacac cgttacccac ggcgatatcg acctgggcct
    17401 tctgttcaac accaacggtt ttatgctgga agaaaacgtc gtcagcacca aaccgcgttt
    17461 ccactttatc gcggacaaac aaaacgatat ttcgtcgatt gtggtggaac tggattaccc
    17521 ggtagatatc agcgaagttt cccgggtgat ggaaaacctg ctgctggaat cggcggataa
    17581 actgctgcgt tacaaaggga tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca
    17641 gggcgtccag cgcctctaca gcgccgactg ggaccgcccg tggggcgatg aaaaaccgca
    17701 tagcacgatg gtgtttatcg ggattcagtt gccggaggat gagattcggg cggcgtttgc
    17761 gggactcaaa aagtaaccag ccctccaaca tcacaatacc tgagacttta tcaccctcat
    17821 attcctccag aatatgaggg cattcgggct taattatctc tgaacgtgca acacttccta
    17881 cgactcctta tgcgcctcca catcctgctt cgttaaccag agcgacttac gaccaaaatt
    17941 ttcgtacatt ttcagctgat cttcatagtg ctgatcaact gttccatcgg gagcaataaa
    18001 cccactctga ccgggtgcga ccacatccca ggcaagcacc ggacgatcgc ttgtcgttgg
    18061 tgagaaaaca atcatatcgt tttctgttcc acggttttga tactccgcct gatgacgcgt
    18121 ttcttccgct gcggcctgcg gtacatcaaa gaaattattt gcccggaacg ttaaggccat
    18181 tgcaggtgtt ttccagttac tcacattatt gccatagcgt ttggaaagag tctcccaggt
    18241 atcttccagc gcagccaaca caacctcctg ctgtggtttc ccagcaaaca gatcaaccgc
    18301 ctgtgggatt ggtgatttgt ctccctgcac cgcctcatac aaaatttttg ctccaacact
    18361 tatattcagc gaaccagttg ggccgtcctg ggttgtttcg tagccactgg cgctgtacca
    18421 cttatcaaat ggcataggta cggcagccac tacggtacgc ttcaacatac tggtcagcca
    18481 aacgttcagg atggcagagc ctggctgctg ccaggtttta ccatcatcat taagcaaatt
    18541 gatgccatcc caacgtgtta atgtatccac caactgacgg cgcggatcgc tctgtgtcaa
    18601 accagatgtc gctgcttgca gagtaggtaa aaaaagcctc aggttaagat cctgacgact
    18661 ggtttggcga ataacatccc atgcctgatc agcagttaag cgtggctttt gctcaagcag
    18721 tcggtcgatc tccgtaacgc ga
//
LOCUS       DAJTSL010000075        18032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTSL010000075 DAJTSL010000000
VERSION     DAJTSL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.075"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..1557
                     /gene="cas3"
                     /locus_tag="OO433_002291"
                     /pseudo
     CDS             1..1557
                     /gene="cas3"
                     /locus_tag="OO433_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
     gene            1655..3217
                     /gene="casA"
                     /locus_tag="OO433_002292"
     CDS             1655..3217
                     /gene="casA"
                     /locus_tag="OO433_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HCR6050751.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRSWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATFIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVCLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPDELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            3214..3761
                     /gene="casB"
                     /locus_tag="OO433_002293"
                     /pseudo
     CDS             3214..3761
                     /gene="casB"
                     /locus_tag="OO433_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
     gene            3776..4854
                     /gene="cas7e"
                     /locus_tag="OO433_002294"
                     /pseudo
     CDS             3776..4854
                     /gene="cas7e"
                     /locus_tag="OO433_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
     gene            4865..5611
                     /gene="cas5e"
                     /locus_tag="OO433_002295"
     CDS             4865..5611
                     /gene="cas5e"
                     /locus_tag="OO433_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCR6050752.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSLSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            5593..6243
                     /gene="cas6e"
                     /locus_tag="OO433_002296"
     CDS             5593..6243
                     /gene="cas6e"
                     /locus_tag="OO433_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6050753.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDISVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            6240..7160
                     /gene="cas1e"
                     /locus_tag="OO433_002297"
     CDS             6240..7160
                     /gene="cas1e"
                     /locus_tag="OO433_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCR6050754.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLQVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            7160..7453
                     /gene="cas2"
                     /locus_tag="OO433_002298"
     CDS             7160..7453
                     /gene="cas2"
                     /locus_tag="OO433_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR6050755.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(7660..8697)
                     /gene="iap"
                     /locus_tag="OO433_002299"
     CDS             complement(7660..8697)
                     /gene="iap"
                     /locus_tag="OO433_002299"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6050756.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTTEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            8949..9857
                     /gene="cysD"
                     /locus_tag="OO433_002300"
     CDS             8949..9857
                     /gene="cysD"
                     /locus_tag="OO433_002300"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6050757.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            9859..11286
                     /gene="cysN"
                     /locus_tag="OO433_002301"
     CDS             9859..11286
                     /gene="cysN"
                     /locus_tag="OO433_002301"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6050758.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMSWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     PSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            11286..11891
                     /gene="cysC"
                     /locus_tag="OO433_002302"
     CDS             11286..11891
                     /gene="cysC"
                     /locus_tag="OO433_002302"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6050759.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            11941..12264
                     /locus_tag="OO433_002303"
     CDS             11941..12264
                     /locus_tag="OO433_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6050760.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            12458..12769
                     /gene="ftsB"
                     /locus_tag="OO433_002304"
     CDS             12458..12769
                     /gene="ftsB"
                     /locus_tag="OO433_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6050761.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            12788..13498
                     /gene="ispD"
                     /locus_tag="OO433_002305"
     CDS             12788..13498
                     /gene="ispD"
                     /locus_tag="OO433_002305"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6050762.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            13498..13977
                     /gene="ispF"
                     /locus_tag="OO433_002306"
     CDS             13498..13977
                     /gene="ispF"
                     /locus_tag="OO433_002306"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6050763.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            13974..15023
                     /gene="truD"
                     /locus_tag="OO433_002307"
     CDS             13974..15023
                     /gene="truD"
                     /locus_tag="OO433_002307"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6050764.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQHRVNDKELMITAALPGSGEWGTQRKALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            15004..15765
                     /gene="surE"
                     /locus_tag="OO433_002308"
     CDS             15004..15765
                     /gene="surE"
                     /locus_tag="OO433_002308"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6050765.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            15759..16385
                     /gene="pcm"
                     /locus_tag="OO433_002309"
     CDS             15759..16385
                     /gene="pcm"
                     /locus_tag="OO433_002309"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6050766.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            16525..17616
                     /gene="nlpD"
                     /locus_tag="OO433_002310"
     CDS             16525..17616
                     /gene="nlpD"
                     /locus_tag="OO433_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6050767.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSY
                     SGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGG
                     NAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTA
                     PVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAI
                     IATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMG
                     STGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            17679..>18032
                     /locus_tag="OO433_002311"
     CDS             17679..>18032
                     /locus_tag="OO433_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; this sigma factor controls a
                     regulon of genes required for protection against external
                     stresses; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6050768.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLD"
BASE COUNT         4414 a         4329 c         4964 g         4325 t
ORIGIN      
        1 tttaatgaat cactctggtc cggtgattta atcgggtcag aagaaccgga tgaacaagct
       61 ttcagccagg gttgtgcggc ctagtttgcc gacaataaca agaaggcgtt gctggctgaa
      121 attggcgtcg gcaccctgga tcaggcgatg atggcagtga tgccgtttaa acataataat
      181 ctgcggcttc tggggctgag taacaaaatc ctgctggctg atgagatcca cgcctgtgac
      241 gcttatatgt cgtgcattct tgaagggctg atcgagcggc aggcgcgtgg cggaaacagc
      301 gttattttgc tttctgctac gttatctcaa cagcagcgcg acaaactcgt cgccgccttt
      361 gcgcgtggtg cagagggcca gcaagaagcc ccactcctgg gaaaagatga ttacccctgg
      421 ctgacgcatg tcacgaaaac cgatgtacat tcgcaccggg tagcgacgcg caaagaagtt
      481 gaacgtagcg tgagcgtggg ttggctccat agtgaacaag agtgtattgc gcgtatcgaa
      541 tcggcggtaa gtcaggggaa atgcatcgcc tggattcgga attctgttga tgacgctatt
      601 caggtgtatc gtcagctgct tgcccgcggc gtcattcctg cttccagcct ttcacttttt
      661 catagccgct ttgcttttag cgatcgccag cgaattgaaa cggaaacgct ggcacgcttt
      721 ggtaaatatt gtagtttaca gagagcgtct caagtcattg tttgtacaca agtaattgaa
      781 caaagcgtgg atattgacct ggacgaaatg atctccgacc tggcccccat tgatttgcta
      841 atccagcgag cggggcgatt acagcggcat atccgcgata ttaatggtca gttaaagcgt
      901 gacggaaaag acgagcgttc cccccctgaa ttgctgattc tggcccccgt ctgggatgac
      961 gctcctggtg acgaatggtt cggcagtgcc atgcgtaaca gtgcatatgt ctatcccgat
     1021 catgggcgaa tctggctgac gcagcgtgtg ctgcgtgagc aaggcgctat tcagatgccg
     1081 cacgcagccc ggcttcttat tgaatcagtt tacggtgagg acgtggtaat gccggaagga
     1141 tttgcccgca gcgagcagga gcaagtgggc aaatattact gtgatcgcgc aagggctaaa
     1201 aagtatgtgc tgaacttcag gctcggctat gccgccaata tcaacgatta ccttccggaa
     1261 aaactgtcga cacgtctggc tgaagagtct gtttccctgt ggctggctac ctgtattgac
     1321 ggtgtggtga agccttatgc caccggtgct cacgcatggg aaatgagcgt tgtcagagtg
     1381 cgccgaagct ggtggaaaaa acatcgggat gagttttctt tactggaagg ggatgcgttc
     1441 aggcagtggt gcgttgaaca gcggcaagat ccggaaatgg caaacgtgat tttagtcacc
     1501 gatgacgaaa gttgcgggta ttcggccagg gagggattga ttggcaaggt tggttgagat
     1561 gtctgcgaca agactctctg aatagttgat atacagaaaa ggatggaagg atggacaacc
     1621 gacctgggat ggtctttttt acgagaggat gcgtatgaac tcgttttcac ttctgacaac
     1681 cccgtggttg cccgttcgtt ttaaagacgg aacgacaggc aaactggcac cagtcgatct
     1741 ggctgatgaa aatgttgtcg atatcgctgc gccgcgggca gatctccagg gggcggcctg
     1801 gcagtttttg ctggggctac tacaaaccag cttcgcgcca aaagatcaac gtagttggga
     1861 tgatatctgg gaagacggac tggaagctga aaagctaagg gaagcattgc tgtctttaga
     1921 acatgctttt cagtttgggc cagattcccc ttcatttatg caggattttg atgaattaaa
     1981 agttaaggca acttttattg cttcgcttct tcctgacgca ccaggaaaac aaacaaaaga
     2041 acgaaataca gaccatttta ttaagcgaga cacaactcag catctgtgcc tgcattgtgt
     2101 gcctttagcg cttttttcaa tccagttgaa tgcacctata ggtggacgcg gttactatcc
     2161 tgggctacgt ggcggagggc ctctgacgac acttatagaa ttgctggagt atcagggcaa
     2221 tcaacaaacc cctttgtggc gaaagctgtg gctaaacgta atgccgcaag atgaagccga
     2281 tttaccttta ccaaaaacct ttgatgacct ggtctttcct tggcttgcac cgacgcgtac
     2341 cagcgaactg gacggtgctg tggtaacgga tgaacaagtc aataaacttc aggcctactg
     2401 gggaatgcca cgacgtatac gtattgattt taagacaacc tcaataggta actgcgacat
     2461 ttgcggtagg cagagcgatg ctcttttggg cttgatgtca ctcaaaaact atggtgttca
     2521 atacgtcatg tggcgccatc ctctaacacc ttaccgtctg ccgcttaaag agggtggaga
     2581 tttttactca gttaaaccac aacctggtgg tttaatctgg cgcgactggt taggcctcat
     2641 agaggtaggt aactcaaaaa acaatactga actccccgca caggtcgtga aacttttgaa
     2701 tgccagcaat ctaaagcaga caagggtgtg cttatgggga tttggttttg attttgaaga
     2761 tatgaaggtt cgttgttggt atgaacacca tttcccgctg ttgttaaaca aaaaagaaga
     2821 cctaatacct aagttgcgtc tggcagcgca agcggcatca cacattctaa gcttgttaca
     2881 ccgagcgttg aaagaagcat ggttctctga gaagaaaacc acaaagcttg atttcggttt
     2941 tgtggatatt gacttctgga acaaaactca gcatcgattc ctgagattag tgcgcaaaat
     3001 tgaagaaggt caggatccgg acgaattact cagtaaatgg caaaaggaaa tgtggttatt
     3061 cgcgcgtcag gattttgacg atcgtgtctt taccaatcca tatgagcccg ttgatttgga
     3121 acgcgtcatg accgcacgca agaaatattt tacaacatca gcggagaagc aaaatgctaa
     3181 cgccgccagg gagaaaaagc aggaggctgc tgaatgagta ttgtaaaaga tgagcataaa
     3241 gttgcgctgc gtaaatggca tgaagagttg caggataaac gcggcgagcg tgccagtctg
     3301 cggcgtagta cgacggttaa tgatgtttgt ctgacggatg gatttcgcct gtttcttaag
     3361 aacagacaaa taaagtggca ggatgaaccc gagtggcgta tcaccgcact agcgctgata
     3421 gcagcagtta gcgccaatgt taaagctatt gacgaacggc agccttttgc cgcgcaactg
     3481 gcggcggtaa tgaaaaaggg gcggtttact cgtttgttgg cagtaaaaac gccggatgat
     3541 ttattgcggc agttacgccg tgcggtcaaa ttattaaacg gttccgtcaa tcttgactct
     3601 ctggaagagg gggtcttccg ctggtgccag gagagcgatg acctgctcaa tcatcaccgc
     3661 cgccagcagc gaccaacaga atttatccgt atccgctggg cgctgggcgc tgggcgctgg
     3721 aatattacca ggctggcgac gccgataacg aacaaaatta ataaaggact atctcatgac
     3781 aacatttatt cagcttcatt tgttaaccgc ttaccctgct gccaatctta accgtgatga
     3841 ttccggctct ccgaaaactg tgattctggg tggagcaacg cgtctgcgcg tttcctcgca
     3901 aagtctgaag cgtgcttggc gcacttctga actttttgaa caggcgctgg cgggccatat
     3961 tggtgttcgt agcgggcgta ttgcgcgtga ggcggcaact atcctgattg agaaaggaat
     4021 cgaagataaa aaagccatcg aatgggcagt agagatagcc gattatcttg ggaaagccaa
     4081 aaaagacaaa aaacaaaaaa acgacaaaaa accaaaagat ccgctcacta gcgccgaaac
     4141 tgaacagctc gtccatatca gcccggcaga atttgacgcc gtaaaagcgc tggcccatca
     4201 actggcagaa gaaaagcgcg cgccaaaaga gaaagatctc gctttgttac gtaaagaccg
     4261 tatggcggta gatatcgcta tgtttggtcg tatgctggcg aaaaaacccg ggtttaatgt
     4321 agaagccgcc tgccaggtcg cgcatgcgtt tggtgtcagt gaaacgattg tcgaaaatga
     4381 ttttttcacc gccgtagatg atttacgcca ggcttctgaa gatgccggtg ccggtcatgt
     4441 agatgaaacc ggattcggtt ctgcgctgtt ctacacctat atcttatctg cattgataaa
     4501 gatctgctgg tagaaaacct cggcggtgac gaagcgttag ctaatcagac catacgcgcc
     4561 tttacggaag ccgcgcttaa agtctcccca accggtaaac aaaacagctt tgccagccgg
     4621 gcttatgcct cctgggcgct ggcagaaaaa ggcaccgatc aaccgcgttc tctggcggca
     4681 gctttctatg aacccattaa tggcacccgt cagttagagg tggcggtgca gcgtattaca
     4741 actcttcgcg aaaatatgaa tacggtctac gaacagaaga ccgattacgc aagttttgac
     4801 gtgatgaaca aacagggaag catgaaggac gtgctggact ttatctgcgc gtaaggggcg
     4861 gcttatgagc caatatctga tttttcagct tcatgggccg atggcatcct ggggcgtcga
     4921 tgcccccggc gaagtgcgtc atacccatga actgccttcg ctctcagcat tgctggggct
     4981 gctggctgcc ggggtaggga tccggcgtga tgataccgaa cgcttaaacg cgtttaatcg
     5041 ccactattca ctggtggttt gcgccagccg taacccgcgc tgggcacggg attatcacac
     5101 ggtccagatg ccaaaagagg tgcgtaaagc acgttatttc agccgtcgcg aagagttgag
     5161 cgaccctgag cttttgagcg cgattatctc ccggcgcgac tactacaccg atgcctggtg
     5221 gatggtggcg gtggcaacaa cagccgatgc gccttacagc ctcgaacagt tgcaggacgg
     5281 tttacgccat ccagtttttc cgctttatct ggggcgaaaa agtcatccgc tggcattacc
     5341 tcttgcgccg ttactgctcg aaggcaacgc gtgtgatgcc ttatgtaatg cataccaaca
     5401 gtatcaggat catttccata aactgaaagt ttcactcccg aaacttcagg atgaatgctg
     5461 gtgggaaggg aaacacgacg gcctggtcgc gagcaaaata ttacgccgcc gggatgttcc
     5521 tttaaatcgt cagcagtggc tgtttgggga acgcaccgtc aatcaggggc cgtggctcag
     5581 caaggaggaa ccatgtacct ctcaagaata accttacata ccggccagct ttcgcctgcg
     5641 cagttgctgc atctggtgga tcgcggtgaa tatgtgatgc atcagtggct gtgggatctc
     5701 ttccctagcg gcaaagaaag gcaatttctt tatcgtcggg aagaactcca gggcgcgttt
     5761 cgcttttttg tactgtcgca ggaacgcccg gcggaaagtg atacgttcac catagaatgc
     5821 cggtcatttg cgcccgagct gtgtaccgga caacaacttt gtttcaacct gcgggcgaat
     5881 ccaacaatct gtaaagcggg caagcgccac gatctgctga tggaggcgaa acggcaggtg
     5941 agggggcagg cggaaggaag tgatgtctgg ttgcatcaac aacaggcggc gctggactgg
     6001 ctggcagcgc agggggagag aagtggtttt acactgctgg atatctcggt tgatgcctac
     6061 cgacaacagc aactgcggcg ggaaaactcc cggcaactga tccagttcag tagcgtggat
     6121 tatacgggga tgcttacggt caccgatccc gggttatttc tgcaacgtct cagtcagggg
     6181 tatggcaaaa gccgcgcgtt cggctgtggt ctgatgctga tcaaacccgg agcagaggcg
     6241 tgacgtttgt accgctgagc ccgatcccgt taaaagatcg cacctctatg atcttcctcc
     6301 agtacgggca aatcgacgta ctggacggcg ctttcgtgct gatcgataaa accgggatcc
     6361 gcacgcacat tccggtggga tcggtcgcct gcattatgct cgaaccggga acgagagttt
     6421 cccacgcggc ggtgcatctg gccgccacgg tggggacgct acaggtctgg gtcggtgaag
     6481 cgggtgttcg cgtctactct tccggacaac ccggtggggc gcgtgctgat aagttactct
     6541 accaggcaaa gctggcttta acggaagatc tacgtctgaa ggtggtgcgc aaaatgtatg
     6601 aattacgttt tcgtgagcca ccgccagctc gccgttcagt ggagcagcta cgtggaattg
     6661 agggatctcg cgttcgccag acgtatgcat tactggcgaa acaatatggt gtgaaatgga
     6721 atggtcgcaa atacgatcct aaagactggg aaaaaggcga tgttgtgaat cgctgcatca
     6781 gtgctgctac atcatgtctg tacggtatat ctgaagcggc agtattagcc gcgggatatg
     6841 cgcccgctat tgggtttatc catagtggca aaccgctttc atttgtttat gacatagccg
     6901 atatcattaa atttgattcg gttgtaccaa aggcatttga aatagcggcg aggcaacccg
     6961 cagaacctga taaagaagtc agattagcct gtcgtgatat tttccgtagc actaagttaa
     7021 cgggcaaatt aataccgtta attgaggaag tccttgccgc aggtgaaatt gaaccgccac
     7081 aacctgcgcc ggatatgtta ccgccagcca tccccgaacc tgaaacgttg ggcgatagcg
     7141 gtcatcgagg acgcggctga tgagcatggt cgtggttgtt acagaaaatg tcccgccacg
     7201 cttacgtgga cggctcgcaa tctggctact ggaagtgcgt gccggtgtgt atgttggtga
     7261 tacatcaaaa cgtattcggg agatgatctg gcagcaaatt acccaactgg ctggttgcgg
     7321 aaatgtggtg atggcctggg cgaccaatac cgagtcgggt tttgaatttc agacctgggg
     7381 agaaaacaga cgtattccgg tggatttgga tgggttacgt ttggtttctt ttcttcctgt
     7441 tgataatcaa taagttatcc gttctttaaa aataaggaaa tgttttaatt tagttggtag
     7501 attgttgatg cggaataaat ttgtttaaaa acagttatgt atgcttagtg tgttccccgc
     7561 gccagcgggg ataaaccggg cgcactggat gcgataatgg atatcactta gaattccccg
     7621 cccctgcggt agaactccca gctcccattt tcaaacccat caagacgcct tcgccagctc
     7681 cttcaccaac ggtagcatta tccgcataac gtcacggcag cgacgttcta ttcttccagg
     7741 aagtgcctta tcaatatgct gttgattatc cagtcttacg tcatgccagc tatttcccgc
     7801 agggaatgcg gctgtttttg cgcgttgctg ataaccatcc ttattcccaa gattccagtt
     7861 agtcgcttcc accgaaagta cagcaatgcc cgctttgtcg aatatttctg cgtcattaca
     7921 acacccagtg cctttcggat aatttttatt caaccccgga ttggtcgttg cggctattcc
     7981 gtgactgcgc gcaattgcca gcgccctgtc gcgcgttaat ttccttactg cttcagtggt
     8041 ttttacaccg ctgttgaaat acaatttatc gccaacaatt aagttatcga gattaatcac
     8101 cagcagcgta tttttctttt cggtgtcact catccgcttg agtaaattct cagcgcctaa
     8161 tttcccttcc tcttcgccgc tggtcgccac aaatcgaata ccatactcgg taggcgtatt
     8221 tttcaggcgt tctgccaatt ccagcatgac acctaaacct gcggcgttat catccattcc
     8281 ttgtaacgtc agcccgccga gattggcatc ggcgtcagca tcgctcagcg gggcgtaagt
     8341 atccagatgc gccataatga tgatctgctg cggcgctttt ccttcatgag cggcaatcac
     8401 cgtacttccc gtcacgttat gccagctctt acgattatcg cgggcggtat aaatataccg
     8461 actattaaat gtccgaatat cactgcgata acccatttgc tgaaactgtt ggcgaatata
     8521 atcggcagat aacatttctg caggagttcc ggtcatgcgt cccggaaaga aagtagcaat
     8581 atgtcgtgcc tgagtattag caaaatcgcc aggtttaggt gacgaggcgt gtacggggag
     8641 aataaagcat acgccgagcg ccagggcagc ggtacggtgg cgcaatgcgg aaaacatagt
     8701 gagtccttaa ataccatgca aattttttta ccgccatagt atgaaactgc cgctgcgcta
     8761 aaacaatttc aaatcttcct aaacgcccga aatccggtgc cttaagcact ttttgatatt
     8821 agctttgcca aatcgttatt ccgttaagga actactcatt ctaattggta atttcattcg
     8881 ttctcttacg ctccctatag tcgaaacatc tgatggcaag aaaatagcgg tattgcaaag
     8941 gaacggttat ggctcaaaaa cgacttactc acctgcggca actggaggcg gaaagcatcc
     9001 acattattcg cgaggtggcg gcagaattct caaatccggt gatgctctac tctatcggta
     9061 aagattccag cgtcatgctg catctggcgc gcaaggcgtt ttatccaggt acgctgcctt
     9121 tcccgttgct gcatgtcgat accggctgga aattccgcga gatgtatgag ttccgcgatc
     9181 gtactgctaa agcctacggc tgcgagctgc tggtgcataa aaacccggaa ggcgtggcga
     9241 tggggattaa tccattcgta cacggcagcg cgaaacatac cgatattatg aaaactgaag
     9301 gcctgaaaca ggcgctgaac aaatacggtt ttgatgccgc cttcggtggt gcgcgccgtg
     9361 atgaagagaa atcccgcgct aaagagcgaa tctactcttt ccgtgaccgc ttccatcgct
     9421 gggatccgaa aaatcagcgc ccggagctgt ggcacaacta caacgggcaa attaacaaag
     9481 gtgaaagcat ccgcgtcttc ccgctctcta actggaccga gcaggatatc tggcaataca
     9541 tctggctgga aaatatcgac attgtgccac tgtatctcgc tgcggaacgt ccggttctgg
     9601 aacgcgacgg tatgttgatg atgattgatg acaaccgtat cgacctgcaa ccgggcgaag
     9661 tgattaaaaa acggatggtg cgtttccgta cgctgggctg ctggccgctg accggtgcgg
     9721 tggagtcaaa tgcacaaaca ctgccggaga tcatcgaaga gatgctggtt tctaccacca
     9781 gtgaacgtca gggccgcgtg attgaccgcg accaggcggg gtctatggag ctgaaaaaac
     9841 gtcaggggta tttttaagat gaacaccgca cttgcacaac aaatcgccaa tgaaggcggc
     9901 gtcgaagcct ggatgattgc gcaacaacat aaaagcctgc tgcgttttct gacctgtggt
     9961 agcgtcgatg acggcaaaag taccctgatt ggtcgcctgc tgcacgatac ccgccaaatc
    10021 tacgaagatc agctctcatc gctgcataac gacagtaagc gtcacggcac ccagggcgaa
    10081 aagctggatc tggcactgct ggtggacggc ctgcaagctg agcgcgaaca gggcatcacc
    10141 attgacgtgg cttaccgtta tttctctacc gagaagcgta aatttattat cgccgacacc
    10201 ccagggcacg agcagtacac tcgcaatatg gcaactggcg catcgacatg tgaactggcg
    10261 atcttactga tcgatgcccg taaaggcgtg ctcgatcaaa cccgtcgtca cagttttatc
    10321 tccacactgt tggggatcaa acatctggtc gtggcgatca acaaaatgga tctggtggat
    10381 tacagtgaag agacgttcac ccatattcgt gaagattatt tgacctttgc cgggcagctg
    10441 ccgggtaatc tggatatccg ctttgtgccg ctctctgcac tggaaggcga caacgtggca
    10501 tcgcaaagtg aaagtatgtc gtggtacagc ggtccgacac tgctcgaagt gctggaaacc
    10561 gtggagatcc agcgagtggt ggatgcccag ccaatgcgct tcccggtgca gtacgttaat
    10621 cgcccgaatc tcgattttcg tggctacgcc ggaacgctgg catccggtcg cgtggaagtc
    10681 gggcaacgtg tcaaagcgct gccctctggt gtggaatcaa acgtcgcgcg gatcgtgact
    10741 tttgatggtg atcgcgaaga agcctttgcc ggagaagcga tcaccctggt gctgacggat
    10801 gagatcgaca tcagccgtgg cgatctgctg ctggcggcag acgaagcgtt accggcggtg
    10861 ccgagcgcgt cggtggatgt ggtatggatg gcggaacagc cgctttcccc gggccagagt
    10921 tacgacatca aaattgccgg taagaagacg cgcgcgcgtg ttgatggcat tcgctatccg
    10981 gttgatatta ataaccttac ccagcgtgaa gttgaaaacc tgccactgaa tgggatcggc
    11041 ctcgtggatc tcacttttga cgagccgctg gtgttagatc gttatcaaca aaatccggtg
    11101 acgggtgggc tgatttttat cgatcgcctg agcaacgtga ctgttggtgc cggtatggtg
    11161 catgagccga aaaacgaggc cgccgttgcg tcatctgaat tcagtgcatt cgaactggaa
    11221 ttgaatgccc tggttcgtcg ccactttccg cactggggcg cgcgcgattt gctgggggat
    11281 aaataatggc gctgcatgac gaaaacgtcg tctggcatag ccatccggtc actgtgcaac
    11341 aacgcgagct acaccacggt catcgtggtg tagtgctgtg gtttaccggc ctctctgggt
    11401 ccggtaaatc aacggtcgcc ggggcgctgg aggaggcgtt acataaactc ggcgtcagta
    11461 cgtatctgct ggatggcgac aatgttcgcc acggattatg cagcgatctc ggttttagcg
    11521 atgccgatcg taaagagaat atccgtcgcg tcggtgaagt ggcgaatttg atggttgaag
    11581 ccggactggt ggtgctgacc gcatttatct cgccacaccg cgccgaacgc cagatggttc
    11641 gcgaacgcgt aggagaaggg cgctttatcg aagtgtttgt cgatacgccg ctggcgattt
    11701 gcgaagcccg cgatcccaaa ggcttatata agaaagcgcg tgccggtgaa ctgcgcaact
    11761 ttacgggaat agattccgtt tacgaagcgc ctgaatcggc agaaattcat ctcaatggtg
    11821 aacaattagt aacaaatttg gtacagcaat tattagatct gttgagacag aacgatatta
    11881 tcagatcctg agacaccacc gggtttccat gcccggttcg tcaaagtaac aggatcaaat
    11941 atgcgtaata gccataacat tacactaaca aataacgaca gccttactga ggatgaagaa
    12001 accacatggt cactgcctgg tgccgtggtc ggttttatct cctggttatt tgcgctggcg
    12061 atgccgatgt tgatttatgg ctctaacacg ctgttcttct ttatctacac ctggcctttc
    12121 tttctggcac tgatgcctgt cgcggtagtg gtggggattg cgctgcattc attgatggac
    12181 ggaaagctac gctacagtat tgttttcacc ctggtgacag taggcattat gtttggcgca
    12241 ctgtttatgt ggctactggg ctaatttgta ctttcttgtc tcctctgttc ataattcaaa
    12301 ccgtaactaa taatgagatt atgttctgca cgccctaggt atacgtaaca atggacaaat
    12361 gtggtacatt tgcccgcgtt gtcgcggtat ccccaacaga ggatgtagag tcgtcttcgg
    12421 atgcatggga tgatgatgcc gtttttcagg gggcaggatg ggtaaactaa cgctgctgtt
    12481 gctggctatt ctggtctggc tacagtattc gctgtggttc ggtaagaacg gtatacatga
    12541 ctatacccgc gtcaatgatg atgtggcggc acagcaagct acaaacgcga aacttaaagc
    12601 gcgaaacgat caactttttg ccgaaattga cgatctcaat ggcggccagg aggcgctcga
    12661 agagcgtgcg cgtaatgaac tcagcatgac caggccgggc gaaacttttt atcgtctggt
    12721 gcctgacgcg tcgaagcgcg cacagtctgc ggggcaaaac aatcgataaa tcagcccggg
    12781 aattaacatg gcaaccactc atttggatgt ttgcgccgtg gttccggcgg ccggatttgg
    12841 ccgtcgaatg caaacggaat gtcctaagca atatctctca atcggtaatc aaaccattct
    12901 tgaacactcg gtgcatgcgc tgctggcgca tccccgggtg aaacgtgtcg tcattgccat
    12961 aagtcctggc gatagccgtt ttgcacaact tcctctggcg aatcatccgc aaatcaccgt
    13021 tgtagatggc ggtgatgagc gtgccgattc cgtgctggca ggtttgaaag ccgctggcga
    13081 cgcgcagtgg gtattggtgc atgacgccgc tcgtccttgt ctgcatcagg atgacctcgc
    13141 gcgattgttg gcgttgagcg aaaccagccg cacgggaggg atcctagccg caccagtgcg
    13201 cgatacgatg aaacgtgccg aaccgggcaa aaatgccatt gctcataccg ttgatcgcaa
    13261 cggcttatgg cacgcgctga cgccgcaatt tttccctcgt gagctgttac atgactgtct
    13321 gacgcgcgct ctaaatgaag gcgcgactat taccgacgaa gcctcggcgc tggaatattg
    13381 cggattccat cctcagttgg tcgaaggccg tgcggataac attaaagtca cgcgcccgga
    13441 agatttggca ctggccgagt tttacctcac ccgaaccatc catcaggaga atacataatg
    13501 cgaattggac acggttttga cgtacatgcc tttggcggtg aaggcccaat tatcattggt
    13561 ggcgtacgca ttccttacga aaaaggattg ctggcgcatt ctgatggcga cgtggcgctc
    13621 catgcgttga ccgatgcatt gcttggcgcg gcggcgctgg gggatatcgg caagctgttc
    13681 ccggataccg atccggcatt taaaggtgcc gacagccgcg agctgctacg cgaagcctgg
    13741 cgtcgtattc aggcgaaggg ttataccctg ggcaacgtcg atgtcactat catcgctcag
    13801 gcaccgaaga tgttgccgca cattccacaa atgcgcgtgt ttattgccga agatctcggc
    13861 tgccatatgg atgatgttaa cgtgaaagcc actactacgg aaaaacttgg atttaccgga
    13921 cgtggggaag ggattgcctg tgaagcggtg gcgctactca ttaaggcaac aaaatgattg
    13981 agtttgataa tctcacttac ctccacggta aaccgcaagg caccgggctg ctgaaagcca
    14041 atccggaaga ctttgtggtg gtggaagatt tgggctttga gcctgatggt gaaggtgagc
    14101 atattctggt tagaatcctc aaaaacggct gcaatacccg ttttgtggcg gatgcactgg
    14161 cgaaattcct gaaaattcat gcccgtgaag tcagcttcgc tgggcaaaaa gacaaacatg
    14221 ctgttacgga acagtggtta tgcgctcgcg tgccaggcaa ggaaatgccc gatctgagcg
    14281 cctttcaact ggaaggctgc caggtgctgg agtatgcgcg gcacaagcgc aagctgcgtt
    14341 taggcgcgct gaaaggtaac gcctttaccc tggttctacg cgaagtgagc aatcgcgatg
    14401 acgttgaaca acgtctgatc gatatttgcg taaaaggtgt accgaactac ttcggtgccc
    14461 aacgttttgg gattggcggt agcaacttgc agggcgcgct gcgctgggcg caaaccaata
    14521 ctccggtgcg cgatcgcaat aaacggagtt tttggttgtc ggcagcccgc agtgcgttgt
    14581 ttaatcagat tgttgctgag cgcctcaaaa aagcagacgt taatcaagtt gttgacggcg
    14641 atgcgctaca attagccgga cgtggtagct ggtttgtagc aaccaccgaa gaactggcgg
    14701 aattacagca tcgcgtcaac gataaagagc tgatgataac cgccgcattg ccgggcagtg
    14761 gcgaatgggg aactcagcgt aaagcgctgg cattcgaaca agcagctgtc gccgcagaaa
    14821 ctgaattaca agctttactg gtgcgcgaaa aagttgaagc cgcgcgcaga gcgatgctgc
    14881 tgtatccgca acaattaagc tggaattggt gggatgacgt caccgtagag atccgtttct
    14941 ggcttccggc gggtagtttt gcaaccagcg ttgtcaggga acttatcaac acaacaggtg
    15001 attatgcgca tattgctgag taatgatgac ggggtacatg cacccggtat acaaacgctg
    15061 gcgaaagcct tgcgtgagtt tgctgacgtt caggtggtcg cccccgatcg taaccgcagc
    15121 ggcgcttcaa attctctgac actggaatcc tccctgcgca cgtttacctt tgaaaatggt
    15181 gatattgctg tgcaaatggg aaccccgacc gattgcgtct atcttggcgt gaatgctctg
    15241 atgcgtccgc gcccggacat tgttgtgtcc ggcattaacg ccgggccgaa tctgggggat
    15301 gacgttattt attccggtac ggtagccgcc gcgatggaag gccgtcattt aggttttccg
    15361 gcgctggccg tctcgcttga cgggcataaa cattacgaca ctgccgcggc ggtaacctgt
    15421 tcaattttgc gcgcactgtg taaagagccg ctgcgcaccg gacgtattct taatattaac
    15481 gttccggatt tacccttgga tcaaatcaaa ggtattcgcg tgacgcgctg cggtacacga
    15541 catccggcag atcaggtgat cccgcagcaa gatccgcgcg gcaatacgct gtactggatt
    15601 ggcccgccgg gcggtaaatg tgatgccggt ccggggaccg attttgctgc ggtagatgag
    15661 ggctatgtct ccatcacgcc gctgcatgtg gatttaactg cgcatagcgc gcaagatgtg
    15721 gtttcagact ggttaaacag cgtgggagtt ggcacgcaat ggtaagcaga cgcgtacaag
    15781 cacttctgga tcaattacgt gcgcaaggta ttcaggatga gcaggtgctg aatgcacttg
    15841 ctgccgtgcc gcgtgaaaaa ttcgttgatg aagcgtttga acaaaaagcc tgggacaata
    15901 tcgccttgcc gataggtcag gggcagacaa tttcgcagcc gtatatggtg gcgcgaatga
    15961 ccgaattact cgagctgacg ccgcagtcgc gggtgctgga aattggcacc ggttcgggat
    16021 atcaaacggc aatcctggcg catcttgtcc agcatgtttg ctcggtcgaa cggattaaag
    16081 gtttgcagtg gcaggcgcgt cgccgcctga aaaatcttga tttacataat gtttcaaccc
    16141 gtcatggcga tggatggcaa ggttggcagg cacgcgcgcc gtttgacgct atcattgtta
    16201 cggcggcacc gccggaaatt ccaactgcgc taatgacgca gctggacgaa ggcgggattc
    16261 tcgtcttacc cgtaggggag gagcaccagt atttgaaacg ggtgcgtcgt cggggaggcg
    16321 aatttattat cgataccgtg gaggccgtgc gctttgtccc tttagtgaag ggggagctgg
    16381 cttaaaacgt gaggaaatac ctggattttt cctggttatt ttgccgcagg tcagcgtatc
    16441 gtgaacatct tttccagtgt tcagtagggt gccttgcacg gtaattatgt cactggttat
    16501 taaccaattt ttcctggggg ataaatgagc gcgggaagcc caaaattcac cgttcgccgc
    16561 attgcggctt tgtcactggt ttcgctatgg ctggcaggct gttctgacac ttcaaatcca
    16621 ccggcaccgg tcagctccgt taatggcaat gcgcctgcaa ataccaattc tggtatgttg
    16681 attacgccgc cgccgaaaat ggggacgacg tctacagccc agcaaccgca aatccagcca
    16741 gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt ctataaccgt
    16801 cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt gaaaaaaggc
    16861 gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct tgctcagcgc
    16921 aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt gggtaatgct
    16981 tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc agagcaagga
    17041 gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc gacaattacg
    17101 tattctgaat cttcgggtga acagagtgct aacaaaatgt tgccgaacaa caagccagct
    17161 gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga gccaactgtc
    17221 agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga gggcaaagtg
    17281 atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc aggcagcaaa
    17341 ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgccggtaa cgcgctgcgc
    17401 ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc ctacgcccat
    17461 aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa aatagcaacc
    17521 atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta caaggggaaa
    17581 tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag gcttttgctt
    17641 gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat acgctgaaag
    17701 ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt tttgacgaaa
    17761 aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa ctgttatcgc
    17821 agggagccac acagcgagtg ttggacgcga ctcagcttta ccttggtgag attggttatt
    17881 caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg cgtggagatg
    17941 tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa attgcccgcc
    18001 gttatggcaa tcgtggtctg gcgttgctgg ac
//
LOCUS       DAJTSL010000076        17503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTSL010000076 DAJTSL010000000
VERSION     DAJTSL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.076"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..744
                     /locus_tag="OO433_002312"
     CDS             <1..744
                     /locus_tag="OO433_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6050769.1"
                     /translation="SSTDKPAKLKFHQYARATLLSNRGRDHNCDGRRTVEIHKLDLSD
                     WQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLL
                     QDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYI
                     DQLHTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRD
                     IRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(815..1264)
                     /gene="rplI"
                     /locus_tag="OO433_002313"
     CDS             complement(815..1264)
                     /gene="rplI"
                     /locus_tag="OO433_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6050770.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="OO433_002314"
     CDS             complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="OO433_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6050771.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1538..1852)
                     /gene="priB"
                     /locus_tag="OO433_002315"
     CDS             complement(1538..1852)
                     /gene="priB"
                     /locus_tag="OO433_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6050772.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="OO433_002316"
     CDS             complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="OO433_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6050773.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2581..2856
                     /locus_tag="OO433_002317"
     CDS             2581..2856
                     /locus_tag="OO433_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6050774.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2931..3482)
                     /locus_tag="OO433_002318"
     CDS             complement(2931..3482)
                     /locus_tag="OO433_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCR6050775.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(3579..4265)
                     /gene="ulaF"
                     /locus_tag="OO433_002319"
     CDS             complement(3579..4265)
                     /gene="ulaF"
                     /locus_tag="OO433_002319"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6050776.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4265..5119)
                     /gene="ulaE"
                     /locus_tag="OO433_002320"
     CDS             complement(4265..5119)
                     /gene="ulaE"
                     /locus_tag="OO433_002320"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6050777.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="OO433_002321"
     CDS             complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="OO433_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6050778.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYQRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="OO433_002322"
     CDS             complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="OO433_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6050779.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="OO433_002323"
     CDS             complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="OO433_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6050780.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="OO433_002324"
     CDS             complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="OO433_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6050781.1"
                     /translation="MSAQHSGCAEYPVTSSSGDRLYNIFTVFFNQVMTNAPLLLGIVT
                     CLGYILLRKSVSVIIKGTIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISD
                     TYASMMATIDRMGDAYSWVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGL
                     IAVTLFIFGYSMWTTIICTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASW
                     IAYKVAPFLGKKEESVEDLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAM
                     AGKVNWTVYILQTGFSFAVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAA
                     IYSFAPNAVVWGFMWGTIGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFG
                     GWRAALKICLVMGMIEIFGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMA
                     VIIVIALAYMFFAGRALRAEEDAEKQLAEQSA"
     gene            8340..9404
                     /gene="ulaG"
                     /locus_tag="OO433_002325"
     CDS             8340..9404
                     /gene="ulaG"
                     /locus_tag="OO433_002325"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6050782.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQAAPQEIRVQWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9512..10267
                     /gene="ulaR"
                     /locus_tag="OO433_002326"
     CDS             9512..10267
                     /gene="ulaR"
                     /locus_tag="OO433_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6050783.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERVGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="OO433_002327"
     CDS             complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="OO433_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6050784.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11195..11524
                     /gene="bsmA"
                     /locus_tag="OO433_002328"
     CDS             11195..11524
                     /gene="bsmA"
                     /locus_tag="OO433_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6050785.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11673..11948
                     /gene="yjfN"
                     /locus_tag="OO433_002329"
     CDS             11673..11948
                     /gene="yjfN"
                     /locus_tag="OO433_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6050786.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12065..13690)
                     /locus_tag="OO433_002330"
     CDS             complement(12065..13690)
                     /locus_tag="OO433_002330"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6050787.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSLLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMCHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13774..14832)
                     /locus_tag="OO433_002331"
     CDS             complement(13774..14832)
                     /locus_tag="OO433_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6050788.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
                     QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQW
                     VEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGN
                     VTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIRED
                     NTLITKDTSRFVPHYIAG"
     gene            complement(14835..15473)
                     /locus_tag="OO433_002332"
     CDS             complement(14835..15473)
                     /locus_tag="OO433_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6050789.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWLNTSGDYSWRSGSGKKESYSSGGFTTKKASTISRGGYGRSSSARGHWGG"
     gene            complement(15483..15881)
                     /locus_tag="OO433_002333"
     CDS             complement(15483..15881)
                     /locus_tag="OO433_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR6050790.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAVSLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15899..16558)
                     /locus_tag="OO433_002334"
     CDS             complement(15899..16558)
                     /locus_tag="OO433_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCR6050791.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16609..17307)
                     /locus_tag="OO433_002335"
     CDS             complement(16609..17307)
                     /locus_tag="OO433_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCR6050792.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSR
                     AQDVLARLQRQQGE"
     gene            complement(17380..>17503)
                     /locus_tag="OO433_002336"
     CDS             complement(17380..>17503)
                     /locus_tag="OO433_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCR6050793.1"
                     /translation="QKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEIT"
BASE COUNT         4466 a         4677 c         4187 g         4173 t
ORIGIN      
        1 tcgtccaccg acaaaccagc aaaattaaaa tttcatcaat acgcacgcgc tacattactg
       61 tcaaatcgtg gaagagatca taattgcgat ggcagacgta ctgttgagat ccacaaacta
      121 gatctctctg actggcaagc attcaataag ttagcaacga agtgtaatgc ttacgatgga
      181 gtaacaatga atggtgataa ttcttttgga tggaaccatg aagctacatt agataatatt
      241 cacgcccaga aatacaataa agcttacgcg ggtgcgaggt taaccgcaga actgaaatat
      301 ttattgcaag atgtcgaatc cttcgaaccc aacagtaaat acacaataca cgaagtggtt
      361 ttgggacctg gttatggtac tccagactat accggccaaa cgataggata cgttgtaacc
      421 cttcctgccc aaatgcctaa ttgctggtca tctgaactac caacaatcga tctttatatc
      481 gaccaacttc acactgttac aggcgtttct aatgccctcg gattcatcat tgctgcattg
      541 ctaaacgcgt attctgattt accccatgat ttgaaaattg gtctgcgttc cttatcaaat
      601 tcagcagcaa tctactcggg tttaggtttc gagcgagtgc cgcaggaccg agacataagg
      661 agtgatcgta tgcatctaac gccagcaaac catcctgact tatggacaca agagaatggt
      721 gaatggatct acttgcgaaa ctaatacctg acgaaaaata gaaagcaaaa cgccgaccaa
      781 tggtcggcgt ttttacgtct cgttgaataa cgaattattc agctactacg tttacgatca
      841 ctttcgcgaa tacttcgctg tgaacctgga agctcacttc gtgttcgcca gtggtacgca
      901 gaacgccgtt cggcagacga acttcgctct tagccacttc aacgccagct gcagttacag
      961 cgtcagcgat gtcgcgagta ccgatggaac cgaacagttt accttcgtcg ccagctttag
     1021 acgcgatggt aacagtttcc agtgcattga ttttctcagc gcgagcatta gcagctgcca
     1081 gaacttcagc cagtttagct tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt
     1141 tcttggtagc tggaacagct ttaccctgcg gtaccaggaa gttacgagca tagcccgctt
     1201 taacgtttac ctgatcacct aggctaccca ggtttgctac tttatcaagc agaataactt
     1261 gcattacctt atcctctcaa agtcgtatta atggaccgtg accgattact gatggcgatc
     1321 agtgtacggc agcagggaca ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg
     1381 gtattttgca cgggtaccgg tgatacggct tgggacaatc ttaccgcttt cggtgatgta
     1441 gtttttcagc gtagcgatat ctttatagtc gatctcttga acgccttccg cggtgaaacg
     1501 gcagaacttg cgacgacgga aataacgtgc catatggcta gtctccagaa tctatcaatt
     1561 caatctgctc ggcatgcaaa accattttgc tcagtccgtt ctttgccttg tggcatgaaa
     1621 tgaacccctg aacggttatg cgactgccga ccgttatact gtgagtaatg gcctggtttt
     1681 cgtgtccgct aacaataacg ggcatttggc accacgcctg ccggtgaaaa ccggcttcct
     1741 cctgcacaga acgatgctca agcacgaact ggcagtgagg aattcctgat ggactgacct
     1801 ttcgaagggg agtcctgcac acggtgccgg acaacaccag acggttggtc atcagaaatt
     1861 actcttcaga atccccagct tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac
     1921 gctcacggcg ctcgtctttc gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg
     1981 tacgcataac catgctgcgg ataacggcat cgttgaagcg gaaggtagtt tccagctcat
     2041 cgatcacttc ctgcggagct tcaacgttca tcagaacgta gtgtgctttg tgcagtttgt
     2101 tgatcgggta agccagctga cggcggcccc agtcttccag acggtggatc ttgccttctg
     2161 caccagtgat ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg ctctggtcag
     2221 gatggaccat aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg gattattcag
     2281 cctcctgtct gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa
     2341 aaatgacgcg ttatagtact tgtatgcccc actaaactca aggccagccg acaaataaat
     2401 ctcatctttc gctcaaaaga actaactcgc tgatttgaag ttcatcacat tccgtcacag
     2461 tgttttttcg aacaactgca tcagaaatgg tcaccatcaa cttctttatc attgattaaa
     2521 attaaatcag aagatcatca cagaggagag cacacaaagt cagccgtcga ggaggtatct
     2581 atgttcagtc gtgttttagc ccttctggct gtgcttttgc taagtgcaaa tacatgggca
     2641 gccattgaaa ttaataacca ccaggccaga aatatggacg acgtacagag cttaggcgtg
     2701 atttatatca atcataattt cgccactgaa agcgaagcac gtcaggcatt aaatgaagag
     2761 acagatgcgc agggcgcaac gtactaccac gtaattctga tgcgggaacc ggggagtaac
     2821 ggcaatatgc acgccagcgc ggatatttat cgctagcacc aggtataacc aacgaaacat
     2881 tgccatagtt tgctttgccc ccttcgcagg gggctttttt atacggttat tcaccggatt
     2941 gcgacttccg gatctggctc atgagtaatg cgattacgta agtcgcggcg cataatttct
     3001 attatccaga accagaaaat atgccccagg atttcagagg catattcgtc aaaaggcaat
     3061 tgagtgagcg gcggcgttaa attcagcgcc gggcaaaata taccgtggac tgccactgtc
     3121 acaataagcc ctgctaatat cccctgccat aatttcactt tcgggaaaat ttccgcgaca
     3181 atacaatacc ctatcgcaaa aaccagtgag aagatgatat gcgtcaccat caccgggttg
     3241 ataatatgtt ccgagaatgt atacacagtt tgggttgggt cgatacctaa ataatcccgt
     3301 agaaagatat acggcgggtt aaattcatcg cgaccaccgg aaaatgtgcg cggtggcaag
     3361 gggacttccg ctccccattt aacaaatgct gaaataattc ctgaaagtac acccacaagt
     3421 aacgcaacgc catagcggcg acgttgcgga tccgttctga caaacaatcc attgatagcc
     3481 ataaaaacac ctgttgttta tacgattttt taactgaagc ggccccggaa tcagaccaat
     3541 tacatcccgc aggaggtaat aattccgggg cgcactgctt acttctgccc gtaataggcg
     3601 ttaggaccgt gcttacgcat gaagtgttta ttcatgagga agctgtcgat gtgattcagt
     3661 tgtgggttaa tactgcgcgc aatccacgcc attttcgcca cttcttccat caccaccgcg
     3721 ttatgcaccg cgtcgtgggc atctttcccc caggcgaacg gcccgtgctg atacaccaca
     3781 attcccggcg tatgcagcgg ctcggcgtca cccagcgttt cgataatcac tttgccggtg
     3841 ttcagttcat actcgccctg cacctcttct tcgcttaatc cacgcgtaca cgggatgtcg
     3901 ccaaagaagt agtcggcgtg cgtggtgcct aacgccggga tcgccagccc cgcctgcgcc
     3961 catgcggtgg catgagtgga atgggtatgg acaatgccgc cgagcgacgg gtaacgacgg
     4021 tagagttcaa gatgcgtcgc ggtgtcagaa gacgggcgat acttcccttc taccaccttg
     4081 ccgctcatat cgaccaccac catatcgtct gctttcatgg tttcgtaggc aacgccgctg
     4141 ggcttgatca ccaccagccc gcgttcgcgg tcgatagcgc tgacgttgcc ccaggtaaag
     4201 gtcaccagcc catagcgcgg cagatccatg ttggcttcaa acacctgctg ttttagcttt
     4261 tgcattatgc cgcctccacc atgcctgctt tggccatgcg cgctttcacc caatcacgcg
     4321 ctttcaccac ttctgccgcc gggtcttccg ccgtttcgct ccacatctca atcaggtacg
     4381 gcccgcaata gccactctgt ttgagcgttt cgaagcaacg ttcgaaatcc accacacctt
     4441 cgccaaatgg cacgtttttg aagacgcccg gtttggtgtc tttcacatgc accgcgacga
     4501 tatgcccgat tccggcctgc aactccatct gcacatcgtt gtcccacgcc gacaggttgc
     4561 cgatatctgg gtagagctgg aaccacgggt tgttgagata gtgcgcgtaa cccagcgcct
     4621 tgctgatgga gttcatcaac ggataatcca tgatctccat cgccagcgtc acctgcgcgc
     4681 ggctcgccat ctcaacgctc tctttcaggc cgtcacggaa acgacgacgc gtttcgttat
     4741 tggcttcctg atagtaaacg tcatagcccg ccagctggat cacgcgaatg ccaacgtcct
     4801 gggcgaactg gatggcttta cgcataatct ccagcccctg cgcccgcacc gcgtcatctt
     4861 cgctgcccaa cgggaaacga cgatgggcag aaaggcacat ggacggcaca cgcacgccgg
     4921 tttcaacaat cgcattcacc agcgccagac gctgctcgcg gctccagtcg aggcgcgaca
     4981 ggcgctcgtc agtttcatct accgacatct cgacaaaatc gaagcccaac gtttttgcca
     5041 gtcgcaggcg ttccagccag cactccccgg cggggagcgc tttttcatag atgccaagcg
     5101 ggatttgttt ggacaacata tccgctcctt agccccacag ttcggcgatg gaacgtttga
     5161 actgacgcgc ggcttcaacc ggagaagcgg catcacggat gctgcgaccg gcgataaaca
     5221 catgaattgg aatgcctttg aacagcggca gatcttccag cgccaggcca ccggtgacgg
     5281 tgactttgaa gcccatatcg gaaagacgtt tgatcgcagt gatatcggct tcaccccacg
     5341 ccacgccagc ggcctgcgcg tcacggctgc gctgataaac cacctgctga atacctgcgt
     5401 cgcgccactg ttgcgcctgt tcccaggtcc agtaaccggt cagttcgatc tgcacgtcgc
     5461 cgttaaactc ttttgctacg tccagcgcac ctttggcggt gttgatatcc gcacaacaaa
     5521 tcacggtcac ccagtcagcg ttggcttcga agcacatacg agaaagaatt ttgcccgcat
     5581 cggcaatttt ggcgtctgcc agcacgattt tgtgcgggta gagcgctttc agatcgcgaa
     5641 ccgcacgcac gccttcgccc acgcacagaa tggtacccac ttcgataatg tcgacttctt
     5701 cggcaatcag gcgagtggtt tcgtaggcgc tatccatagt ctggttgtcc agcgcgactt
     5761 gcaacatcgg taatgacatc ttcaattcct tcttaagctg ccgcgttggt gcggtcaatg
     5821 agatccagta cttcctgctc ggtacggcag gcacgtaaac ggtcgaaatt ttcttcatct
     5881 tcaaacaggt tgacgatctg catgatgccc acttcctggt gcgtgttggc atcgaccgcc
     5941 gccatggtga tgaggatatc caccgggtcg ttatcttcgt ggttgaactc cagcggcttt
     6001 ttcagcgtca ccagcgcaaa accggttttc ttaacgccct cttccgggcg accgtgcggc
     6061 attgccaggc ctggtgcgag cacaaagtac ggaccgtgct gttcaaccgc atccagaatc
     6121 gcctggtagt aacgcggctc gacaacatcc gccgccacca gcaggtcaac gccgattttt
     6181 actgcgtcct gccatgtctc agcttcagcc tgcaggcgga tggatttatt ctccgccagc
     6241 gaatcacgta atttcatggc gcgtccttac ttcacatcct gcgggaaatg cgctttgatc
     6301 acttccagca gtttcgggcc aaagtcggca ggagagagca tgttgcgcac gccaaccacg
     6361 tatttgttgc cggtcacggt gatttcgccc gcaatgtgcg tagaagcgat gatgatatcc
     6421 gcgccgctca actcgctttt gtattcgcca accgcgcagc tatttaccgt gtggtcaatg
     6481 tttgattggg ttaaaaactg gtccactttc atcttcatga tcatggaact gccttgtccg
     6541 ttgccacaca cagccagaat acgtacggtc ataatcaaaa ctccttatta agcagactgt
     6601 tctgccagtt gtttttctgc atcttcttct gcgcgcagtg cgcggccagc gaagaacata
     6661 taagccagtg caattacaat gatgacggcc ataaaggcga taccgatgga gaagaagccc
     6721 tgcatcatcg gcggtgccag aatcgaccag tccgccatgc ccatccaggc gctcataccg
     6781 gtgagtttca ccgcccagac gcagccaaag atttcaatca tccccatcac cagacatatc
     6841 ttcagcgccg cacgccagcc gccgaagtgg ttagcgaaca cgccgatggt ggcgttggag
     6901 aagaacatcg ggataaagcc gggaataatc aggatcgagg aaccgcaggc gaccaggatg
     6961 ccaaccgcaa tcagctgacc gatggtgccc cacataaagc cccaaaccac ggcgttcggc
     7021 gcgaagctat agatagccgc acagtcaatc gccagaaccg caccagggat caggcgctgg
     7081 gagataccgt taaatgcttc agagagttcc gccacaaaca tgcgcacgcc ctgagtgatg
     7141 atgaagatcg ccaccgcgaa ggagaaacca gtttgcagga tgtacaccgt ccagttcact
     7201 ttgcctgcca tcgcctgcac ggtgtcgata ccgaaggaga gtagaatggc accaaagaag
     7261 atggtcatca caatcgccgt ggagacgatg ttgtcgtgga aaatattcag ccagcctggc
     7321 agtttgaggt cttcaacgct ttcctctttt ttgccgagga acggcgcaac tttataggca
     7381 atccatgatg caaactgttg ctggtgaccg atggagaaac cgcagccgtc cgtcacttcc
     7441 tgagtcggct tgtacatcat attggaggta atgccccagt agagcgaaac cagaatcgcc
     7501 gtgcagataa tggttgtcca catggagtag ccgaaaataa acaacgtaac ggcaatcagc
     7561 cccgcctgct ggaacatgat atgaccagtc agcatgatgg tacgaatacc ggtaatgcga
     7621 cgcagcagca cgtaacagat gttcagcgcc agcgctaaca acaccgcgta acctacccag
     7681 ctataggcat cgcccatgcg gtcgatggtt gccatcatcg acgcgtaagt atcggaaatt
     7741 gcgccgttaa tgccgtagac ttcggacatt ttcgccacca ccggtttgaa ggtgctggtg
     7801 aggatgccgg accctgcctg caacaacatg aaaccaatta tggttttaat cgtgccttta
     7861 ataataacgc tgacactttt gcgcagtagg atgtagccca gacaggtcac aatacccagc
     7921 aacaacgggg cattggtcat gacctggtta aaaaacacgg taaagatgtt gtagagacga
     7981 tctcctgaag aagaggtaac cgggtattcc gcacacccgg aatgttgtgc actcactcta
     8041 attttcaaaa gcaatcacaa caagattatt tatgattaaa tgtgacgcga cccgcaaata
     8101 attccatacg gatagagtgg gatccacaca acgaaacaat tatttattta aatttagtta
     8161 aattcattta gttaatttaa ctatacattc cattaaatct ttccacactc aaaatcaaca
     8221 acaacttccc tggcaaaaag tcaaaaaatg gtcattgcct tcctgaaaaa atagtgtcag
     8281 gattaatcaa aaccaatcac atattgattc gaattgaact atcatgaagg taaatggcga
     8341 tgagtaaagt gaaaagtatc acccgagaat cctggatcct gagcactttc ccggagtggg
     8401 gtagctggct gaatgaagaa attgaacaag agcaggtcgc ccctggcaca tttgcgatgt
     8461 ggtggctagg ctgcaccggg atctggttga aatcagaagg tggcaccaac gtttgcgttg
     8521 atttctggtg cggcactggc aaacaaagtc acggtaaccc gttaatgaaa cagggtcacc
     8581 agatgcagcg catggctggc gtgaaaaaac tgcagccaaa cctacgcacc accccgtttg
     8641 ttcttgatcc gtttgcgatt cgccagatcg acgcggtact ggcgactcac gatcacaacg
     8701 atcatatcga cgttaacgtc gctgctgccg tgatgcagaa ctgtgctgat gacgtaccat
     8761 tcattggacc gaaaacctgc gttgatttgt ggattggctg gggcgtaccg aaagagcgtt
     8821 gcatcgtggt caaaccgggc gatgtggtga aagtgaaaga cattgaaatt catgcgcttg
     8881 atgctttcga ccgtactgca ctgatcaccc tgcctgccga tcaaaaagcg gctggcgtac
     8941 tgccggacgg catggacgat cgcgcggtga actatctgtt caaaacgcct ggcggctccc
     9001 tgtatcacag cggcgactcc cactactcta actattatgc gaagcacggt aacgaacatc
     9061 agatcgacgt ggctttaggt tcatacggcg aaaacccgcg cggtatcacc gacaaaatga
     9121 ccagcgccga tatgctgcgt atgggtgaag ccctgaatgc gaaagtggtt atcccgttcc
     9181 accacgatat ctggtcaaac ttccaggccg ctccgcaaga gatccgcgtg cagtgggaga
     9241 tgaaaaaaga tcgtctgaag tatggcttca agccgtttat ctggcaggtt ggcggcaaat
     9301 ttacctggcc actggataaa gacaacttcg agtaccacta tccgcgcggt ttcgatgatt
     9361 gcttcactat tgaaccggat ctgccgttca aatcattcct gtaatctgtc gtttataccg
     9421 gatagcgtgg agaagttcgc gctatccggc gtagtatttc ctggctattt caaatatcat
     9481 ctaaaaaaat caaattttat cggaatagct catgactgaa gcacaaagac atcaaatcct
     9541 cctggaaatg ctcgcacaat tgggctttgt gaccgttgag aaagtcgttg agcgtctggg
     9601 aatttcgcct gccactgcgc gacgcgatat caataaactt gacgaaagcg gcaaactcaa
     9661 aaaggtgcgc aatggcgcag aagctattac ccaacagcgc ccgcgctgga cgccgatgaa
     9721 tctgcatcag gcgcagaatc acgatgaaaa agtacgtatt gctaaagcgg cctcgcagct
     9781 ggttaatccg ggcgaaagcg tagtcatcaa ctgcggctcc accgcgtttc tgcttgggcg
     9841 ggaaatgtgt ggcaagccag tgcaaatcat cactaattat ctaccgctgg caaattacct
     9901 gatcgatcaa gaacatgaca gcgtgatcat tatgggtgga cagtacaaca aaagtcagtc
     9961 catcacttta agcccgcagg gcagcgaaaa cagtctctat gccgggcact ggatgtttac
    10021 cagcggaaaa gggctgaccg cagaagggtt gtataaaacc gatatgctga cagcaatggc
    10081 agagcagaag atgctgagcg tggtagggaa actggtggta ctggttgata gcagtaagat
    10141 tggcgaacgc gtgggaatgc tttttagccg tgccgatcaa atcgatatgc ttatcaccgg
    10201 caaaaatgct aacccggaaa tcctgcaaca actggaagcg caaggggtca gcattctgcg
    10261 tgtttaaaga tgctggcgga aaaatgtcac ggcagcatct aacgcctcag gcgtaatgcg
    10321 gtgacgcacg cctggctgcc atgaacaggt tagcagttta tcccgtcccg tctcgcttaa
    10381 ggcctgctgc aaacgtagtg attcgtcggc aggcacaacg tcatcgtcga ggccatgcca
    10441 cagcagtaga ggtctgtccc caagttgttc caggtggttt gtcgcttccc actctgccag
    10501 cggcgcgata atgttattga attcattctg ctgtgctgcc gtttcaggta tcagcggtgg
    10561 aaacagtgaa cgggcgagcg atgtaaaata gcccgatccc atcatgctgg cggtacattt
    10621 caccgtgggg tggcgagcgg taattcccag tgccgtcatc gcgcccatcg acgcgccacc
    10681 gactgccaga cggtcatcaa gcagccagtt ttcttcggct attgccgcac gtaaagtagt
    10741 gaattcctgc atactttgta gcaagatttg ccagaattga tttaaccgcc gcgcttcgtc
    10801 gctactaaaa cggctaccgt gatcgggcgc atccggcatg atcacccgca aaccagcctg
    10861 cgccagcgca acggcaaaat agctatacac caggctggat gaagtaaagc cgtgataaaa
    10921 aattacgcac ggtaacgggg tatctttttg cccgacagga taagcatgaa gaacgggaat
    10981 atctgccagc tcgcgtgatt ctatttcaat catctgtctc tcctttttgt ctggtatgac
    11041 atgaagaaat ccttagcaca agtttctgct ggcggtatta gttcataatg ttgagatgtg
    11101 ggttacgctt tcgtcacgtt ttcattcgaa aatcgcgtac caggtaacaa ttcgggaaca
    11161 ttcccccaaa gttaaattaa taaggcacta cactatggtt agcaggaaac gtaatagcgt
    11221 tatttaccgg tttgccagtt tattattggt gttgatgtta agtgcctgta gcgcactgca
    11281 aggtacgcca cagccagcac cgccagtaac gaaccatccg caagagattc gccgcgacca
    11341 gacgcaaggg ttacagcgaa taggtagcgt aagcacgatg gttcggggtt ctccggatga
    11401 cgcattagca gaaatcagag caaaagctgt cgctgcaaaa gctgattatt acgttgtcgt
    11461 catggtggac gaaaccatcg tgacaggaca gtggtattca caagccattt tgtaccgtaa
    11521 ataaatcaga caacttacat agtcagcttt acattgcttt gcgtccctat gcgtttacct
    11581 gtgcacaata aattaccggc tggaagcctg ggcgtttatt accgcgatgg aatgccctgc
    11641 aacatgtggg gaaacgaaaa atggagctga cgatgaaaca attacttgct tcaccctcgc
    11701 tgcaattagt gacttatcct gcgagcgcca cggcacagtc tgcagaattc gccagtgctg
    11761 actgcgtgac gggcctgaat gaaataggtc agatctcagt tagcaatatt tcaggggatc
    11821 cgcaggacgt ggaacgtatc gtggcgttaa aggccgatga acaaggtgcg tcatggtacc
    11881 gcattattac catgtacgaa gaccagcagc ctgataactg gcgcgtacag gcgatactgt
    11941 atgcatgagt gtcatctaca gcaaatgaac aatgccggat tcggcgcgag cgccttgtcc
    12001 ggcctacaat tttcacatat ttcattgagt taatcgaaac cagcgtcgca tcagtcgtat
    12061 acgcttacac acacactccc cccgtcgccc gcagcaataa atcattctgg atctgctctg
    12121 acaaccgtac gccgccgcgc gtatctaaca tcacctgaca ccacgcctgc gccattggcg
    12181 gagaagcata tttcaacatt tgcgcaccgc agcccagcag gaatagctga tgagtaatct
    12241 ctcgccccag ttcttcagct ggcttacgca gctgctgctg taaacgacga accgcgcgat
    12301 caaaatagcg atcctgccct ttcacttcca caaatgcttc cgacaataag tcgtatacgc
    12361 ccgcttgctt attgagaacg cgcaacacat ccaggcacat aatattgccg gaaccttccc
    12421 aaatactgtt taccggcatc tcccggtaaa gccgcggcag ctcgctctcc tcgcaataac
    12481 caatgccgcc cagcacctcc atcgcttcgg ccacaaacgg catacctctt ttgcagatca
    12541 caaattttgc cgcaggcgta aataaacgcg cccacagggc ttctttggca tcggcacgcc
    12601 ggtcccacgc tcgcgcaaga cgaaacagca acgccgtttg cccttcaagc tgaagtgcca
    12661 tgcgacttaa gacatgacac ataaggggct gttggatcaa tggattacca aaaacatggc
    12721 gttgatgtgc atgataaatc gccagcgaaa atgcacggcg catcatggca tggctaccca
    12781 gggcgcaatc aaaacgcgtc atcccaccca ttttcaggat cagacgaatt ccttcccctt
    12841 cctgccccaa caaccagcca atggcatcct gaaactccac ttcgcaactg gcgttagagc
    12901 gattacccag cttatctttc agccgctcga ggcgaatcgc gttgcgttgc ccatcaggca
    12961 aaaagcgcgg cacaaaaaag caggacaaac cgcccgtagt ctgcgccagc accagatgcg
    13021 catcgctttg cggcaccgag aaaaaccatt tatgccccac cagccgataa gagccatctt
    13081 ccagacgctc tgcacgggtg gtgttgctca tgacatcgga accgccctgc ttttccgtca
    13141 ttcccatgcc aatcaacaaa ccgcgttttt gcccacctgg caataagaga gaatcgtagc
    13201 gatcgctcag cagcggcgtg gtccagtcct gaaacggcgt gggtaacatc tgtaacaaca
    13261 atggcgtggc ggcaaaggtc atcgttattg gacataacga cccagcctca acctgcgcat
    13321 gtaacataaa acgcgccgcg cgcgccacaa atgcgccgga gcgagcgtct tcttcccagg
    13381 caagattgtg cacccgattg gtacataacg cctgcatcag caggtgccag gcggggtgaa
    13441 aacgcacatc gtccagacgg cgtccttgcg catcgtagcg caataattca ggcggattca
    13501 cattcgccag ccgccccagt tcaagagatt cagccgttcc taactgctga ccaatactgg
    13561 ctagaaaatc gctatcccag ccagcacctt cacgcgttac cgcttcacag agcgcgccat
    13621 cagacaggta taagttgctg ttatttaatg gtataggttg attaaaaacg gtgtgagttt
    13681 gccagtgcac tgtgtctccc tccatcaatg gcagtcaccc ttagtatggt cactgccatg
    13741 gattcctgac agaaagagat tgctaaaaca ttcttatcca gcaatgtaat gcggaacgaa
    13801 acgtgaggtg tctttggtga tcagcgtgtt atcttcacgg atccccattc cgcacgcttc
    13861 atcatcgaca atccagctac cgatgagtgt gtagctatcg ccaaatcgcg gcagaggttg
    13921 aaacgcctgg tagatcatcg gttcatcggc gtaatcacca tcagcgtggt caacgacgtt
    13981 attctgaccg tcgaaaatgg tgacgtttcc gccttcgcgt gagtagatcg gtttacgcac
    14041 atagctttcg ccagcggcaa tctgcggttt ttcgccctca aaccaggatg caagaaggtt
    14101 aggatgacca ggaaagaagc gccaaagcag cggcattagc cctttattac tcaagatact
    14161 tttccataac ggctccaccc attgctcgcg acgcttgcgc agcagcggac cgttatcgtc
    14221 acgcatcatc cactccagcg gatacagctt aaatgcacgc tggatgacat tatcatcaag
    14281 atcggtcagt acgccgccga cgcccaaacc gagatcttca atgtagataa accgcgactc
    14341 ctgccctgcc tgctgggcgc agtcctgcaa gtacagcacg gtactcctgt cttcatcggt
    14401 gtcctgacag cagcaaaaat aaaacggttc ccgactgtaa agctcactaa agcgcgaaat
    14461 caggcgttcc tgaatggcgt tgtactccag cagcttcacc ggcgcattgc cacaccaggc
    14521 aaaatccatc cggccataca gcgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc
    14581 ccagtacaac ggcgggatcg ccagttgcgt caggatctct tcgtctttaa ccgcgcgatc
    14641 caccacctca aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat
    14701 ctggcgcaga gtaaaacggt aagcccgact ctcgtcccaa tagatttcat tgtcgatgat
    14761 atgaaagtcg aaaccgttgt tagcggcgat ctggtccaga tcccgtcgca caggaacgtt
    14821 gtgtctcagc atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg
    14881 ccgcgcgaaa tggttgacgc ttttttggtg gtaaagccgc ctgaagagta agactctttt
    14941 ttgccagaac cggagcgcca ggagtaatca ccggaagtgt tgagccagac ggggcgggaa
    15001 gcaaatgagg atccgccgct gttatagacg aacggctcat cgcgatcttt acgaataacc
    15061 cggcttaaca aaaatccaga aacgaccggg atccaactct gttcaacatt gtcgtagtag
    15121 caattttcgt acttagactg acaattctgc tgagtcatat tcttcggaac atcggcataa
    15181 aatgccgctt tggcgttgtt ccagccacgc gcgcaaatgt cggcattatt accgtcatca
    15241 atacaatcct gcactgtcga ataaaacgtt ccgtcgccgt cgttatcgac atcgccacta
    15301 tcgccacaac ctttcaatac aaaaaaagcg gcaccgccca ttagcgccaa cgttaagtat
    15361 ttttgtgcgt agcgattgcg acgcggttca aaaggattat tcggtctacc aatgcgactg
    15421 atcgctccgt gaccaatttt actgttattt cttgatttgc gttttctggc catgatcgtc
    15481 cattaccatg tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc ggttcccata
    15541 aacattcctg ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagt
    15601 ctgacaccag caaaaacgag taactgaatc accagtgcaa tcccgcccca ggcgaaatag
    15661 tctggaatac tcaccgcatt gattgccgca ctggataagg ggatcacgta acccaacaat
    15721 gtaccgctga atgccagtga caccgcggta ttattgtttt tgattaattg ccattcgttg
    15781 tgcggtgtaa ttttggagta gataaacaga aaaataatca ccatagccac gccaataaaa
    15841 aaataggcac taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt
    15901 aaccaataat atgaagtgat gtcagcggga tatcgacacc caacgcgcgc gaaaataacc
    15961 actctggctc gccgagatcg ttaaaagatt cctcaccatt taacagtaaa tattcgtaag
    16021 tatcttcggt tacttgccgc tggtagccca tggtaaaatt atggacctcc catttggcat
    16081 ggttttgatt ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc
    16141 tgttaaaaaa tcgctgccag cgtttttcct gccagtttaa ggtcattgcc cccatcgttt
    16201 tggcgttgat cgcctcgcgc cagtgactct ctttactgat accgtagctc tcttcataga
    16261 caaagagctt gatatcatca atgtcatcaa tatcttcgcc gccggtggta ttgatttgca
    16321 gaaattcatc gcctgaagtg tagtaacgga aaatctggct accgcctccc agatcgatgc
    16381 ggctgacagc ggctaccgta aattcctcac ccggtagcgc aatcagcaat tcatcttcca
    16441 gcaaacgaaa cgctaacgtg tcgagcgtaa agccactatt gagatgaagt cccagcggac
    16501 cgcgagcgat agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat
    16561 tttatcctta aaatagttgc cagcctttta gcgaggctgg caaaaaaatt actcgccctg
    16621 ttggcgttgc agtcttgcta atacatcctg ggctctactt ttattgctac cgccaattcc
    16681 ggcttccgcc agcttttcgt caaggtcgcg accgtctgcg actttctcca actgtgcggc
    16741 agcatccagg cgagcctgac gttcggcctg acgcgtttgc aggcgtttta aggattctgc
    16801 cgctgtcgaa acgctggaag atgcgccaac ggtagaggtt gttacagcct gttgtgcacg
    16861 ctgcatggct tcagtggctt taacgacttc catttgctgc tcaaactgag cgatacgctg
    16921 cgctgtcgct gtaactgctt tttccacgcc atcacgggag acttccagat tcgacaacac
    16981 ttgctcttca gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa cttcgttaat
    17041 caacgacgga ttaacgttct tgcttaacgc ttccagcgca cgggcttcca gactggcttt
    17101 gcgctcacgt aaatctttca gcttatcgtg acttaatttc acccgcgcca gcagatcaac
    17161 gcgagatttt ccggctttat cgagttcagc tttagcgtca cgaatatgct gttccagcat
    17221 gcgcacgccc tgggtttctt caatggattc ttccgcctgg gagataaacg atttccccag
    17281 cgtaaataaa ctttttaaaa ttcccataaa tacccgtcct tgtaattagt gtgaatattc
    17341 ttctgtaatt tcagccagat ccaatgcgtt atctaccagc taggttatct caagcaggat
    17401 atcttcaaga gaagatttaa gcgataacgc cccgaaaaca atgtaatact cttcctgttg
    17461 cacgctggag attcctaccg atgacaacgg catcatcttc tga
//
LOCUS       DAJTSL010000077        17421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTSL010000077 DAJTSL010000000
VERSION     DAJTSL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.077"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..339
                     /gene="fliH"
                     /locus_tag="OO433_002337"
     CDS             <1..339
                     /gene="fliH"
                     /locus_tag="OO433_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6050794.1"
                     /translation="ASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            339..1707
                     /gene="fliI"
                     /locus_tag="OO433_002338"
                     /pseudo
     CDS             339..1707
                     /gene="fliI"
                     /locus_tag="OO433_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
     gene            1726..2169
                     /gene="fliJ"
                     /locus_tag="OO433_002339"
     CDS             1726..2169
                     /gene="fliJ"
                     /locus_tag="OO433_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR6050795.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERLSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            2166..3293
                     /gene="fliK"
                     /locus_tag="OO433_002340"
     CDS             2166..3293
                     /gene="fliK"
                     /locus_tag="OO433_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6050796.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADQNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            3398..3862
                     /gene="fliL"
                     /locus_tag="OO433_002341"
     CDS             3398..3862
                     /gene="fliL"
                     /locus_tag="OO433_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6050797.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALNTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            3867..4871
                     /gene="fliM"
                     /locus_tag="OO433_002342"
     CDS             3867..4871
                     /gene="fliM"
                     /locus_tag="OO433_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6050798.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            4868..5281
                     /gene="fliN"
                     /locus_tag="OO433_002343"
     CDS             4868..5281
                     /gene="fliN"
                     /locus_tag="OO433_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6050799.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMHRLSR"
     gene            5284..5649
                     /gene="fliO"
                     /locus_tag="OO433_002344"
     CDS             5284..5649
                     /gene="fliO"
                     /locus_tag="OO433_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6050800.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWQVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            5649..6385
                     /gene="fliP"
                     /locus_tag="OO433_002345"
                     /pseudo
     CDS             5649..6385
                     /gene="fliP"
                     /locus_tag="OO433_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            6395..6664
                     /gene="fliQ"
                     /locus_tag="OO433_002346"
     CDS             6395..6664
                     /gene="fliQ"
                     /locus_tag="OO433_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6050801.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            6673..7458
                     /gene="fliR"
                     /locus_tag="OO433_002347"
     CDS             6673..7458
                     /gene="fliR"
                     /locus_tag="OO433_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6050802.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            7743..8366
                     /gene="rcsA"
                     /locus_tag="OO433_002348"
     CDS             7743..8366
                     /gene="rcsA"
                     /locus_tag="OO433_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6050803.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(8410..8598)
                     /gene="dsrB"
                     /locus_tag="OO433_002349"
     CDS             complement(8410..8598)
                     /gene="dsrB"
                     /locus_tag="OO433_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6050804.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            8761..8977
                     /gene="yodD"
                     /locus_tag="OO433_002350"
                     /pseudo
     CDS             8761..8977
                     /gene="yodD"
                     /locus_tag="OO433_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
     gene            9275..10090
                     /locus_tag="OO433_002351"
                     /pseudo
     CDS             9275..10090
                     /locus_tag="OO433_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(10087..11781)
                     /gene="yedQ"
                     /locus_tag="OO433_002352"
                     /pseudo
     CDS             complement(10087..11781)
                     /gene="yedQ"
                     /locus_tag="OO433_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(11952..12134)
                     /locus_tag="OO433_002353"
     CDS             complement(11952..12134)
                     /locus_tag="OO433_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6050805.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(12213..13130)
                     /locus_tag="OO433_002354"
     CDS             complement(12213..13130)
                     /locus_tag="OO433_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6050806.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            13303..14223
                     /gene="yedA"
                     /locus_tag="OO433_002355"
     CDS             13303..14223
                     /gene="yedA"
                     /locus_tag="OO433_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6050807.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(14212..14682)
                     /gene="vsr"
                     /locus_tag="OO433_002356"
     CDS             complement(14212..14682)
                     /gene="vsr"
                     /locus_tag="OO433_002356"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6050808.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(14663..16081)
                     /gene="dcm"
                     /locus_tag="OO433_002357"
     CDS             complement(14663..16081)
                     /gene="dcm"
                     /locus_tag="OO433_002357"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6050809.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(16148..16852)
                     /locus_tag="OO433_002358"
     CDS             complement(16148..16852)
                     /locus_tag="OO433_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR6050810.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     VIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGE
                     NEGVDHKVIDAFSPAG"
     gene            complement(16892..17257)
                     /locus_tag="OO433_002359"
     CDS             complement(16892..17257)
                     /locus_tag="OO433_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6050811.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         4172 a         4635 c         4403 g         4211 t
ORIGIN      
        1 gcttcgcgcc tgatgcagat ggcgctggag gcagcacgtc aggtcatcgg tcagacgcca
       61 acagtggata actcggcact catcaaacag atccaacagt tgttgcagca agaaccgtta
      121 ttcagcggta aaccacagct gcgcgtgcac ccggatgatc tgcaacgtgt ggatgatatg
      181 ctcggcgcta ccttaagttt gcatggctgg cgcttgcggg gcgatcccac cctccatcct
      241 ggcggctgta aagtctccgc cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg
      301 caagaactct gccgtctggc agcaccagga gtggtgtaat gaccacgcgc ctgactcgct
      361 ggctaaccac gctggataac tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct
      421 acgggcgatt aacccgcgct accgggctgg tgctggaagc caccggatta caattgccgc
      481 tcggcgcaac ctgtgtgatt gagcgccaga acggcactga aacgcacgaa gtagaaagtg
      541 aagtcgttgg ctttaacggt caacggctgt ttttaatgcc gctggaggaa gtcgaaggtg
      601 tcctgcccgg cgcgcgtgtt tatgccaaaa acatttcggc agaagggctg caaagcggca
      661 aacagttacc gctcggtccg gcgttattag gtcgtgttct ggacggcagc ggtaaaccgc
      721 tcgatggcct cccccgatac gacggaaacc ggtgcgctga ttaccccgcc atttaacccg
      781 ttgcaacgta caccgattga acatgtgctg gacaccggcg tgcgcccaat caatgccctg
      841 cttaccgttg ggcgtgggca gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt
      901 gtgctgctgg ggatgatggc acgttacacc cgcgccgatg tcattgtcgt gggtttgatt
      961 ggtgaacgcg gacgcgaagt aaaagatttt attgagaata tcctcggtgc cgaagggcgt
     1021 gcacgctccg tggtgattgc cgctccggcg gatgtttctc ctctcctgcg aatgcagggt
     1081 gccgcctatg ccacgcgcat tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg
     1141 attatggact ccctcacccg ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc
     1201 gaaccacccg ccactaaagg ttatcccccg tcggtgtttg ccaaattacc ggcactggtc
     1261 gagcgtgccg gaaatggcat tagcggcggc ggatcgatta ccgcgtttta taccgtgctc
     1321 actgaaggcg atgaccagca ggaccccatt gccgactctg cgcgggcaat cctcgacggt
     1381 cacattgtgc tgtctcgccg actggcggaa gccgggcact atccggctat cgatattgaa
     1441 gcgtcgatca gccgcgcaat gacggcgttg atcagtgagc aacattacgc gcgagtgcgc
     1501 accttcaaac agctgttgtc gagttttcag cgtaaccgcg atctggttag cgtcggcgcg
     1561 tatgccaaag gcagcgatcc gatgctcgac aaagccatcg ccctgtggcc gcagctggag
     1621 ggctatttgc aacaaggcat ttttgaacgc gcggactggg aagcgtctct ccaggggctg
     1681 gagcgtattt tcccgacagt gtcataaccc aggagataac ggcagatggc agaacatggc
     1741 gcgctggcga ccctgaaaga tctggcagaa aaagaggtag aggatgccgc gcgcctgctg
     1801 ggtgaaatgc gtcgcgggtg tcagcaggcg gaagaacagc tcaaaatgct gatcgattat
     1861 cagaatgaat atcgcaataa cctcaacagc gatatgagtg ccgggatgac cagcaaccgc
     1921 tggatcaact atcagcagtt tatccagacg ctggaaaaag ccattactca gcatcgccag
     1981 caacttaatc agtggacgca gaaagttgac attgccctga acagctggcg agaaaagaaa
     2041 caacgtttgc aggcctggca gacactgcag gaacggctat ccacggcggc actgcttgca
     2101 gaaaaccgcc tcgatcagaa aaagatggat gagttcgccc agcgcgccgc catgaggaaa
     2161 cctgaatgat tcgcttagcg cccttaatta ccgccgacgt tgacaccacc acattgcctg
     2221 gcggcaaagc cagcgatgct gcacaagatt ttctcgcgtt gttgagcgaa gcattagcag
     2281 gcgagacaac taccgacaaa gcggcccccc agttgctggt ggcaacagat aagcccacga
     2341 caaaaggcga gccgctgatc agcgatattc ttgccgacgc gcaacaagcg gatttactga
     2401 tccccgtgga tgaaacactg cctgtcatca acgacgaaca atccacatca acaccgttaa
     2461 ccaccgctca gacgatgacg ttggctgcgg tggctgacca aaatacgaca aaagacgaaa
     2521 aagcggatga tctgaatgaa gacgtcaccg caagcctgag cgcccttttt gcgatgttgc
     2581 cgggttttga caatacgccc aaagtgactg atgtgccgtc aaccgtgtta ccggcagaga
     2641 aaccaacgct cttcacaaaa ctgacttctg cgcaactcac aacagcacag cctgatgatg
     2701 cccccggcac gccagctcag ccattaacac cgctggtagc agaagcccag agtaaagcgg
     2761 aagtcatcag cacaccgtca ccggtgaccg ctgccgccag cccgctaatt actccacacc
     2821 agacacagcc actgcccacc gtcgccgcgc ctgttttgag tgcaccgctg ggttctcacg
     2881 aatggcaaca atcattaagc cagcatattt cgctgttcac ccgccagggg caacaaagtg
     2941 cagagttgcg tctgcacccg caggatttag gtgaagtgca aatctccctc aaagtggatg
     3001 ataaccaggc gcaaatccag atggtttcac cgcatcagca tgtacgcgcc gcgctggaag
     3061 cagcgcttcc ggtactgcgt acgcagctgg ccgaaagtgg cattcagtta gggcaaagca
     3121 acatcagtgg cgaaagcttt agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc
     3181 aacgcacagt aaaccatgaa cctctggcgg gggaagacga cgatacgctt ccggttcccg
     3241 tctctttaca agggcgtgta acaggcaaca gcggcgttga tattttcgcc taacgtcaga
     3301 ggtagcaccg taatccgcgt cttttccccg ctttgttgcg ctcaaaacgc aggataatta
     3361 gccgataagc agtagcgaca caggaagacc gcaacacatg actgattacg cgataagcaa
     3421 gaaaagcaag cgatcgcttt ggatcccgat tctggtattc attaccctcg cggcctgtgc
     3481 cagcgcaggt tacagctact ggcattcgca tcaggttgcc gctgacgaca aagcgcagca
     3541 acgcgtcgta ccctccccgg tcttctacgc gctgaatacc ttcacggtca atttgggcga
     3601 tgcggatcgc gtgctttata tcggcataac cctgcgcctg aaagatgaag ctacccgctc
     3661 gcggctgagt gagtatttgc cggaagtccg tagccgtttg ctgttactgt tttcgcgtca
     3721 ggatgctgcc gtactggcga cagaagaagg caagaaaaac ctgattgccg agattaaaac
     3781 cacgctttcc accccgcttg ttgccgggca accgaaacag gatgtcaccg acgtgctgta
     3841 taccgctttt attctgcgat aacgacatgg gcgatagtat tctttctcaa gctgaaattg
     3901 atgcgctgtt gaatggtgac agcgaagtca aagacgaacc gacagccagt gttagcggcg
     3961 aaagtgacat tcgtccgtac gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc
     4021 aggcgctgga aatcattaat gagcgctttg cccgccattt tcgtatgggg ctgttcaacc
     4081 tgctgcgtcg tagcccggat ataaccgtcg gggccatccg cattcagccg taccatgaat
     4141 ttgcccgcaa cctgccggtg ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg
     4201 gcacagggct ggtggtgttt tcaccgagtc tggtgtttat cgccgtggat aacctgtttg
     4261 gcggcgatgg acgcttcccg accaaagtgg aaggtcgcga gtttacccat accgaacagc
     4321 gcgtcatcaa ccgcatgtta aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga
     4381 ttaatccgct ggaagttgag tacgtgcgtt cggaaatgca ggtgaaattt accaatatca
     4441 ccacctcgcc gaacgacatt gtggttaaca cgccgttcca tgtggagatt ggcaacctga
     4501 ccggcgaatt taatatctgt ctgccattca gcatgatcga gccgctacgg gaattgttgg
     4561 ttaacccgcc gctggaaaac tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc
     4621 gccaggtgca gcattcacag ctggagctgg tcgccaactt tgccgatatc tcgctacgcc
     4681 tgtcgcagat tttaaaactg aaacccggcg acgtcctgcc gatagaaaaa cctgatcgca
     4741 tcatcgccca tgttgacggc gtcccggtgc tgaccagtca gtacggcact ctcaacggtc
     4801 agtatgcgtt acggatagaa catttgatta acccgatttt aaattctctg aacgaggaac
     4861 agcccaaatg agtgacatga ataatccggc cgatgacaac aacggcgcaa tggacgatct
     4921 gtgggctgaa gcgttgagcg aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt
     4981 gttccagcaa tttggcggtg gtgatgtcag cggaacgttg caggatatcg acctgataat
     5041 ggatattccg gtcaagctga ccgtcgagct gggccgtacg cggatgacca tcaaagagct
     5101 gttgcgtctg acgcaagggt ccgtcgtggc gctggacggt ctggcgggcg aaccactgga
     5161 tattctgatc aacggttatt taatcgccca gggcgaagtg gtggtcgttg ccgataaata
     5221 tggcgtgcgg atcaccgata tcattactcc gtctgagcga atgcatcgcc tgagccgtta
     5281 gtgatgaata accacgctac tgtgcaatct tccacgccgg tttctgctgc gccactgctg
     5341 caggtcagcg gcgcactcat cgccattatt gccctgatcc tcgctgctgc ctggcaggta
     5401 aaacggttgg gatttgcccc gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt
     5461 gcttcgctgg gcgcgcgtga acgggttgtg gtggtcgatg tggaagatgc acggctggtg
     5521 ctcggcgtta ccgcagggca aatcaatctg ctgcataaac ttcccccttc agcaccaaca
     5581 gaagagatac cgcagaccga ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg
     5641 agatcctgat gcgtcgtttg ttgtctgtcg cacctgtcct tctctggctg gttacgcccc
     5701 tcgccttcgc gcaactgccg ggtatcacca gccagccgct gcctggcggt ggacaaagct
     5761 ggtcgctccc ggtgcagacg ctggtgttca tcacctcgtt gacgtttatt ccggcaattt
     5821 tactgatgat gaccagtttc acccgcatca tcattgtttt tggtttattg cgtaacgcgc
     5881 tgggaacgcc ctccgcgcca cctaaccagg tattgctggg gctggcacta tttttgacct
     5941 tttttattat gtcaccggtg atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg
     6001 aagagaaaat atcaatgcag gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta
     6061 tgctgcgtca gacccgtgag gcagatttag ggttgtttgc cagactagcg aataccggcc
     6121 cgttgcaggg acctgaagcc gtgccgatgc gcattttgcg cccggcctac gtgaccagcg
     6181 agttgaaaac cgcatttcag ataggcttca cgattttcat tccttttttg attatcgacc
     6241 tggtgattgc cagcgtgttg atggcattgg ggatgatgat ggtccccccg ccaccattgc
     6301 tctgcccttt aaactgatgc tgtttgtact ggtggatggc tggcaattgc tggtcggttc
     6361 gctggcgcag agcttttaca gctagagagg caaaatgaca cctgaatcgg tcatgatgat
     6421 ggggactgaa gcgatgaaag tcgcgctggc actggctgcc ccgctgttgc tggtggcgct
     6481 ggtcacgggc cttatcatca gtattttgca ggccgccacg cagatcaacg aaatgacgct
     6541 gtcgtttatt ccaaaaatca tcgccgtatt tattgccatt attattgccg gaccgtggat
     6601 gctcaatctg ttgctggatt acgtccgcac cttgttcact aacctgccgt atatcatcgg
     6661 gtaggccgta caatgatgca ggtgacaagc gatcaatggc tttcctggtt aagcctgtac
     6721 ttctggccgt tactgcgcgt gctggcgctc atttctaccg caccaattct gagcgaacgc
     6781 agcgtgccga aacgggtaaa actggggctg gcaatgatga tcacgttcgt cattgcccca
     6841 tcattacctg ccaacgatgt tcctgttttt tcgttctttg ctctgtggct ggccgtgcag
     6901 cagatcctga tcggcattgc gcttggtttt accatgcaat ttgcctttgc cgctgtgcga
     6961 accgctggcg aaattatcgg tctgcaaatg gggctgtcat ttgcgacgtt tgtcgatccg
     7021 gccagccatc tcaatatgcc cgttttagcg cgtatcatgg atatgctggc gttactgctg
     7081 ttcctgacat ttaacggtca tttatggttg atttcactgc tggtcgatac ctttcacacc
     7141 ctgccaattg gtggcgaacc gttgaacagc aatgcgtttc tggcactcac caaagcgggg
     7201 agtttgattt tccttaacgg gctgatgctg gcgttaccgc tcattactct gctgctgacc
     7261 ctgaatctgg cattaggttt actgaatcgt atggccccgc aattatccat ttttgttatt
     7321 ggatttccat taactctgac tgtcggtatc tctttaatgg cggcattaat gccgttaatt
     7381 gcaccttttt gcgaacattt attcagtgag atgtttaatt tgctggctga tattattagt
     7441 gaattgccat taatataatt ccgtaacgtt tatcatgtta tcctaaggat tatccgaaaa
     7501 ataataccta cgaacatctt ccaggatact cctgcggcga aatatttgtt ttaagctcac
     7561 tcacatatcg caacatttac tttaagacaa ttccaggcaa attatacaac actttacggg
     7621 atagtaagtc cgcctgaaaa atcgcgagag tggcgcatta ggtgacccat gttgttccgt
     7681 ttagtcatga tgaaatattc aggtaagggg aattatcgtt acgcattgag tgagggtatg
     7741 ccatgtcaac gattattatg gatttatgta gttacacccg actaggttta accgggtatc
     7801 tgttgagtag aggggttaaa aaaagagaaa tcaacgacat tgaaaccgtt gatgaccttg
     7861 ccatagcttg tgattcacag cgcccttcag tggtgtttat taatgaggac tgtttcatcc
     7921 atgatgcttc taacagtcag cacatcaagc acatcattaa tcaacatccc aatacgttat
     7981 ttatcgtttt tatggcaatt gccaatgttc attttgatga atatctattg gtcagaaaaa
     8041 atttattgat aagttctaaa tcgattaaac cggaatctct cgacgatatc cttggcgata
     8101 ttctgaaaaa agagacaacg ataacctctt ttttaaatat gccgacgtta tcattgagcc
     8161 gaaccgaatc gagtatgttg cgaatgtgga tggcaggtca gggaaccatt caaatctctg
     8221 accaaatgaa tatcaaagcc aagaccgttt catcgcataa aggtaatatt aaacgtaaga
     8281 tcaaaacgca taataaacag gttatctacc atgtcgtccg actgacggat aatgtgacta
     8341 atggtatttt tgtcaacatg cgctaacaca ttctgactgg tggtttccca ccagtcaggc
     8401 tgaataagat tactctgctt tctccacaaa gataccgtcc tgatgccctg cttcattaaa
     8461 gaaccagatg ccgagcgggt agtcttccag cgaaaccagg tacattgtgc cttcactaaa
     8521 ctcctcaact gctagtacca cgccaggacg acgcggaccg ccatccgttt tgactgttac
     8581 ccgatcattc accttcatcg ttttcctcct gtggctttgt gccagtgtag aacaatttcg
     8641 tttttctggc agcgccaggc gcgcgcgagt gctgattttc tcgacggtct atacttaaga
     8701 gatgccaggc ggacttaacg actggcggca acaacagagt aacggttgcg aggaaagatg
     8761 atgaaaaccg caaaagagta cagcgatacc gcaaaacgtg aggtcagcgt cgatgtcgat
     8821 gccctgctgg cggcgatcaa tgaaataagt tcatcgcagc cagaacgatt ctgaacacgt
     8881 tagcgtcgat ggacgtgaat atcatacatg gcgtgaattg gcggatgcct tcgaactgga
     8941 tattcatgac ttcagcgtct ctgaagtgaa tcgttgaatg cacaataaaa aaatcccgac
     9001 cctgaggggg tcgggatgaa acttgcttaa gcaagaagca cttaaaaaat tcgttacacc
     9061 aggaaatctg atgtgttcat caccttatcc gcaatttttt tcgctgacaa gaaaatattc
     9121 gcgatatgaa tgattaacgc tacttatgaa tagaaatgtg acgtgcgtca ccccttggtg
     9181 gctctggcgt caacgggatc gctcccgaaa aaatcatctt tgattatttg tgctgttttg
     9241 agaatcatcc tggtaagggg agaactgcgc gaccatgttt tcaattcaac aaccactact
     9301 ggtttttagc gatcttgatg gcaccctgct ggacagtcat agttatgact ggcaaccggc
     9361 tgccccctag ctcagccgtt tacgcgaagc gaatgttccc gtcattctct gtagcagtaa
     9421 aacttcagcg gaaatgctgt acttgcaaaa aacgttgggg ttacaagggg taccactgat
     9481 tgccgaaaat ggcgcagtga tccagcttgc tgaacaatgg caggatatag acggttttcc
     9541 acgcatcatc tcaggtatta gccatggcga aatcagccag gttttaaata cgctacgcga
     9601 gaaagaacat tttaaattca cgactttcga tgatgtcgac gatgcaacca tcgccgaatg
     9661 gacgggatta agccgtagcc aggcggcgct gacgcagctg catgaggcgt cggtaacgct
     9721 aatctggcgc gacagtgacg agcgtatggc acaatttacc gctcgtctga acgaactggg
     9781 cttacagttt atgcagggtg cgcgcttctg gcacgtcctg gatgcctctg ccggaaaaga
     9841 tcaggccgcc aactggatta tcgcgaccta tcaacaatta tcaggcaaac gcccaaccac
     9901 acttggcctg ggcgatgggc caaacgatgc gcccttactg gaggtaatgg attacgcggt
     9961 gattgtgaaa gggctaaacc gtgaaggggt gcatctgcat gatgaggatc cggcccgcgt
    10021 ctggcgaacg cagcgtgaag gaccggaagg atggcgtgaa gggctggacc attttttctc
    10081 cgctcgttaa gcgttatcgc tcgcgcatac ccgattacgc ccggcctgtt tagcgagata
    10141 aagccgacgg tcagccagtg actggagttg ttcaaaatca taatcaccgg cttcctcgct
    10201 gctacttacc cccaacgagg cactgatgcg tatcgtcgta ctcttagcaa tcaacatctc
    10261 tttttcattt aacttcaggc gaatacgttc tgcgacttcc gcagcctccg tcagactcgc
    10321 gcctggcaga atcacacaaa actcctcacc accgacccgc ccggcaacgt cctgcgcacg
    10381 caaggaactg ctaattaatc cggcagcatg agaaagaaca cggtcgcccg cctgatgact
    10441 aaagcggtca ttaatcgctt taaaatggtc aaggtcgacc tgaatgacag aaaaaggatg
    10501 ttggtgcgtc tgacacaatt tcgcgagcgg acgagctttt tcaaacagtg cgccacgatt
    10561 atataaacgg gttaaggtgt cgtgccacgc ctgccactgc aacgagcttt gcagaacata
    10621 catgttgcta accatccggc gaatcacata ccaggagatg agtaacatgg tggtaaagag
    10681 cgcccacagc agggttaatg caatgctgat actgccgaaa tcgccgcgca cgccttcgct
    10741 tagcgtatgg acgcgcacca gcacaccgtc gaaatggtcc agacgttccc agctaacata
    10801 gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc atcgcctgcg ccagcaaggc
    10861 cagttcacga ggatcaaaaa tattccctgt tggatggtca ggattggaag aggtcaaaaa
    10921 tctcagctta ctgtcataga gctgatactc accatcgagg tttttatcga tggcgtttct
    10981 taaaaattgc tgcatggtac gcacgggaat actcatcccc agcacgccat accagtagtt
    11041 attactgtct accggaacac tgacggtaac ctgcggttca gtattgctgg cgtgttccgg
    11101 ttgcgaagta aaccagcgta ccgcgcggtg acgattttct cattgcgaat ggccgataaa
    11161 ccagggttgg gtgacatagc cgtaataacg cgttggtaca ttgcgcgtaa acaaggtcgg
    11221 ctgcgtcgaa acgtaaaatc cggcacgcga gacatacatc gcctgttcaa ccatcgacga
    11281 ggtgttgtgc gccagtcgca gcaagtaacc aacttccagt gcagcggtaa tttcattgtc
    11341 gaggctttca ttttcgcgag acaggagatt cccctcgctg actaatgcat ccgatacgcc
    11401 attgactgac agggtgcgtc gtcggttgag ttcaatttgc caggcgtgct cgtcgcgatg
    11461 ctgttcgaac tcggttacag cattacgcag tgaagtgaaa tccagtggcg ctacgagagc
    11521 ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg ttatactgca aatgcttatc
    11581 gagcgcgttg gctacgtttt ccagatgatt acgctgactg gagatatagg catcttccag
    11641 cactaccact tcacgccagg tcagcaaggt ggaaaaaagc aataccacga taaagcagag
    11701 attaacgaca tgaccaggcc ccaggcggcg tgcgattttt ttcaaccagc tctggttttc
    11761 catttttgtc tcgtgctgca ccctgacgac tcccaacctg cttttttatg attctggcat
    11821 aacctggcga tagcgtaccg caaaacagcg ataattttat aatggtggca tgataagacg
    11881 cgactatcat tcattgcagg cattggcaca ccaggccgga agtgatccgg cctggtgtta
    11941 atgataccct ttcaaacttc ttccgcagaa cgactcccct cccccggaac aagctcggtt
    12001 tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc aacgacactc aaccataccg
    12061 ctggagtatc cggtgacaat catccgcggg ccgccctctt taaccgtaac ttcctcacta
    12121 accataaagc tcatactcgc ctcctttttt cgagtgaaac ttgttcacct tagttgaaga
    12181 tggcgaattt tgcataatag cctgtgcgag tattaatgtg cctgaccgcg tattttcgct
    12241 acggctttca ctcccagcac cacgatgccg ccgatgataa atccaagaat cagatttaaa
    12301 acagtcggta atatcatcgc caccactgca ctttgctgcc cggcgaaatg ttcaatggcg
    12361 tgatgcagcg gcgcaatacc atgtaccaca atcccgccgc cgacgaggaa catcgccagc
    12421 gtgccgacaa tcgataacgc tttcatcagc cagggcgcaa tgatcaataa ccctttacct
    12481 aatgcctgca tcagcgcgct ggatttttcc gccagccaat accccaggtc atcaatctta
    12541 acgataaccc ctaccagacc gtagacgccc acggtcacta ccagcgcgat gcctgaaagc
    12601 accagcacct gattaagcaa cggcgcttcg gccacaatcc ccagcgtgat ggcgacgatt
    12661 tcggcagaca atataaaatc ggtacgaatc gcccctttta ttttgtcctt ttcaaacttc
    12721 agcggatcct gcgccgccag cttctccaga cgctgctggc tctgcgccgg atcttcttta
    12781 tgtttacgcg cctccagcat atgcagcact ttctctactc cttcaaagca gagaaacgcg
    12841 ccaccaatca tcaacagagg cgtaatcgcc cacgggataa acgcactgat gatcagcgcc
    12901 agcggcacca gaatcacttt attaatcagc gatcctttcg ccacgcccca gaccacggga
    12961 agttcccggt tggcccgcac gcctgaaact tgttgcgcat tgagcgataa gtcatcccct
    13021 aatacaccgg cggttttctt cgccgccagt ttgcccatca cggagatatc gtccagtagt
    13081 gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg ccaaaagcat tccctctcat
    13141 ttttattgtt gaagggtgta agtatgaagc aaaaacggca gctctgatac ttgcttcaca
    13201 ggtcaacatt tttttaacaa tcaagtgcaa ttatatctct cgccagcaga atgattttca
    13261 cgtttactat gaaccacctt tttatttcca ccgtgaggga ttatgcgttt ccgccagttg
    13321 ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt ggggctcaac ctattttgtc
    13381 attcggattg gcgtggaaag ctggcctccg ttaatgatgg caggcgttcg attcctggca
    13441 gccggtattt tattgatggc atttttgcta ctgcgcggac acaagctccc cccgctacgt
    13501 ccgctgctca atgccgcgct gattggcctg ttattgctgg ctgtcggtaa tggcatggtg
    13561 acggttgccg agcatcaaaa tgttccttcc ggcatcgccg ccgttgtggt tgcaaccgtg
    13621 cccctcttta ccctgtgctt cagccgcctg tttggcatta aaacgcgcaa attggaatgg
    13681 gtgggtatcg ccattgggct tgccggaatc atcatgctca atagcggtgg aaatttaagc
    13741 ggcaatccgt ggggcgcgat tctgatttta atcggctcga ttagctgggc gtttggctcg
    13801 gtttatggct cgcgcattac cttacctgta gggatgatgg cgggtgcgat tgagatgctg
    13861 gcggcaggcg tggtgttaat gatcgcgtcg atgattgcag gtgaaaaact gacggcgctc
    13921 ccttcccttt caggcttcct tgcggtcggc tatctggcgc tgtttggttc gattatcgcc
    13981 atcaacgctt atatgtattt aatccgtaat gtcagtccgg ctctcgccac cagctacgcc
    14041 tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac tgggcggaga gacgctgtcg
    14101 aagattgaat ggctggcgct cggcgtaatt gtcttcgcgg tggtactggt cacgttggga
    14161 aaatatctct tcccggcaaa acccgtagtt gcgccagtta ttcaggacgc atcaagcgag
    14221 taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct tcgccgcaga tccactcttc
    14281 cagacgctcg gtaagcgctt catccgtcag cttctcgcgc ccacgtaatg cacactccca
    14341 gacaatcagt acgcgccagc cgagttcctg caaacgactg atatcgcggc gatcgcgttc
    14401 aacattttta cctatcttct ccagccagaa ctcggttcga gtcgcaggca ctttaaacag
    14461 atagcagtga tgatgatgcc agaagcagcc atgggtaaat atcacgcagc gatattcatc
    14521 aacgacaaaa tccgggcttc cgggcagact ggcgtcctga acgcgaaacg ccaggccctg
    14581 cccggttaac agactggcga ggcgcttctc aatcgccgta tcacgcgtgg caatcgcgcg
    14641 catatttttg ctgcgagtgg ccttatcgtg aacgtcggcc atgttgtgcc tcttgctgac
    14701 gcaacgccac cgcctgtttg atttttggct caagcagttt tgccaccgcg gcaaagaccg
    14761 gcacgaccac cgagttaccg aactggcgat aggcctgagt gtccgaaacc ggaatgcgga
    14821 atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc gcattcccga ggcgttaacc
    14881 gacgtggacg atgttgctga ttcagcggat cgtcaaagtc tttctcacct ttggccatat
    14941 cccagccgcg atcgattaaa atttccgcgc catctttgta ataacgcgca gacagcgtac
    15001 gcgtgacgct ttgcggattg ttcggataaa ccattccata accgaagccg ttaccgcgcg
    15061 cctgatgttt tttcgcatat cgatagaggt acttccacag caccggcgtc aggatatatt
    15121 tcgcctcgac catcgggtcc aacagctgcg ccagcgtcac tcgctgtgca gggaaacatt
    15181 cgctgatatc acgcagagta aaatcggctt tcagattaag atcgcgacga aaacccacca
    15241 gcacgatgcg ttcacggtgc tgcggcagaa aatgtttacc atcgatgatt ttcggatcgt
    15301 ccggcccgtt atcttctgca tcagccacgt catagcccag ttcgtccagc gtctgcatga
    15361 tgatgcggaa cgttttaccc tggtcgtgac ttttcaggtt tttgacgttt tcgagcacaa
    15421 acatcgccgg acgacgcgcg tcgataatgc gtaccacatc aaaaaacagc gtgccctggg
    15481 tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt tttcgatacg ccagccagcg
    15541 aaaatggctg acaagggaaa ccggccagta aaacatcgtg ttcaggaatg tgttgacgaa
    15601 tatgttccgc cgccgcctca tcactcacgc cttctttatg gctgagggtg atatcgcgga
    15661 tatcttcatt aaaatgatgc gtcgccggat cgcaataatg gttggcttta taagtgcgta
    15721 ccgcatgttt gttccattcg ctggtaaaca cgcattgtcc gccaatcgat tcaaaaccgc
    15781 gacggatgcc gccaattccg gcaaatagat cgataaagcg aaacgcataa tgcggatgat
    15841 gtgccggtgg tttgggtaac aacgtttgca gatgggcgaa ctctttctca cttaaacggt
    15901 gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc cgcgctccag tgattctcac
    15961 ccacaccatt aagctgcgcc accagcgttt taacatcata aatttgcagc agtttctcca
    16021 gcattgcctg tgcggcattc ccggtgctgt atgaatcggt tactgatata ttttcctgca
    16081 tagatttaac cggccatctg agatggccga caggttaaca taattacagc catttagggc
    16141 cacgcgctta tcccgcgggt gaaaacgcat ctatcacctt gtgatcgaca ccttcattct
    16201 cccccgccag ttcggcactg agcttcgcca taaactcgac taaaaaatgc gcgttgtgct
    16261 gcgccagctg cttgcctctt gcggtttgca tggtttgcat ggtttgcggt agcttcagca
    16321 acttagtctg gaaatgatcc agcgcgtagc gtttatcatc aagcgggcga tgctgtgcga
    16381 acggatcttc accatcaaac agcgccacgc ccaatgcccc tgaaacggca aacacgcgcg
    16441 ccaggccaat cgcccccagg gcttccagcc gatccgcatc ctggacgatt ttagcttccg
    16501 ttgttaaggg ggcgatttgc gcgctgaaac tgtgagcggc aatgacatga caaacggcct
    16561 cgattttctc cgccggaaat tgctcgaact cttcacggag caggcgacgc gtctcttctg
    16621 ccgccaggat tgaagaacgc tgcctttgcg gatgattttt cgccaggctc acaatatcgt
    16681 gaaaataaca ggcggttaaa atcaccagca tatcgacatc gtcatctgcc gccagcttct
    16741 gtgccgtagc ccagacgcgg cgaaaatgac acacgtcatg ggccgcgtcc tggtgctgat
    16801 gatggttttt caaccagttt tcgaactgtg cctgccagtg ttgtaagtcc atacgcgctc
    16861 cgttgccaaa gagtcgccac cttagcagtt tttattcata gcgtctgcta cgtgcgggac
    16921 gaggtcgggt gtaccaggca atgctgccta acaatgcccc gaccagagcc ataaataaaa
    16981 atccgaccag tgcccctaac cactttcccg cggtagagcc taaatcgctt tcaaatcccg
    17041 acacgggggc atcggggatc cggctcatca cccagatgta acgcgccatt gcccagacga
    17101 aatagccaca ccatgtatag aaagcccaca aagccagttt gccgccaggg ctgcgagaga
    17161 gtctctcttc catctttgtc gaaccgtatt ccattttgag ataacccgga aaattcagac
    17221 ttagcactat tatgtgataa aagtcacatt tttccacatt ggatgagcca gtcaagactc
    17281 aatcattttt tgaacaaata gataaatttt gcgcaacggc agtgaataca ggaaaaatcg
    17341 ttttgagcga ttttatttat ctttgatatc ggctttatta attcatttaa tcaatatatt
    17401 agcactgatt acaattatac c
//
LOCUS       DAJTSL010000078        17341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTSL010000078 DAJTSL010000000
VERSION     DAJTSL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.078"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            189..2036
                     /gene="ldtD"
                     /locus_tag="OO433_002360"
     CDS             189..2036
                     /gene="ldtD"
                     /locus_tag="OO433_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6050812.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2217..2765
                     /locus_tag="OO433_002361"
     CDS             2217..2765
                     /locus_tag="OO433_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6050813.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2792..3439
                     /gene="gloC"
                     /locus_tag="OO433_002362"
     CDS             2792..3439
                     /gene="gloC"
                     /locus_tag="OO433_002362"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6050814.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3661..4851)
                     /gene="aspC"
                     /locus_tag="OO433_002363"
     CDS             complement(3661..4851)
                     /gene="aspC"
                     /locus_tag="OO433_002363"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6050815.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARMAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5036..6124)
                     /gene="ompF"
                     /locus_tag="OO433_002364"
     CDS             complement(5036..6124)
                     /gene="ompF"
                     /locus_tag="OO433_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6050816.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYAHLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6726..8126)
                     /gene="asnS"
                     /locus_tag="OO433_002365"
     CDS             complement(6726..8126)
                     /gene="asnS"
                     /locus_tag="OO433_002365"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6050817.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8295..9497)
                     /gene="pncB"
                     /locus_tag="OO433_002366"
     CDS             complement(8295..9497)
                     /gene="pncB"
                     /locus_tag="OO433_002366"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6050818.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9763..12375
                     /gene="pepN"
                     /locus_tag="OO433_002367"
     CDS             9763..12375
                     /gene="pepN"
                     /locus_tag="OO433_002367"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6050819.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12418..13169)
                     /gene="ssuB"
                     /locus_tag="OO433_002368"
                     /pseudo
     CDS             complement(12418..13169)
                     /gene="ssuB"
                     /locus_tag="OO433_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(13166..13903)
                     /gene="ssuC"
                     /locus_tag="OO433_002369"
                     /pseudo
     CDS             complement(13166..13903)
                     /gene="ssuC"
                     /locus_tag="OO433_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714028.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            complement(13968..15113)
                     /gene="ssuD"
                     /locus_tag="OO433_002370"
                     /pseudo
     CDS             complement(13968..15113)
                     /gene="ssuD"
                     /locus_tag="OO433_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15110..16069)
                     /gene="ssuA"
                     /locus_tag="OO433_002371"
     CDS             complement(15110..16069)
                     /gene="ssuA"
                     /locus_tag="OO433_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6050820.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALCIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16062..16637)
                     /gene="ssuE"
                     /locus_tag="OO433_002372"
     CDS             complement(16062..16637)
                     /gene="ssuE"
                     /locus_tag="OO433_002372"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6050821.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            16993..>17341
                     /locus_tag="OO433_002373"
     CDS             16993..>17341
                     /locus_tag="OO433_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050822.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDA"
BASE COUNT         4371 a         4434 c         4398 g         4138 t
ORIGIN      
        1 acggcgcgct tttctttaaa cttctttaca ttaggttatg taaaaacgta tcggcgttta
       61 tatactgaag ataagcctga tgagtaacag gcttgctcgt catactttcg tgagtattgg
      121 cgttgtacag gcaagtcgta aaataacagc ctggctattc agagtatgat aaaaacaggg
      181 ggcaagggat gttgcttaat atgatgtgtg gtcgtcggct gtcggcaatc agtttgtgcc
      241 tggccgtaac attcgctcca ctgttcaatg cgcaggccga tgagcctgaa gtaatccctg
      301 gcgacagccc ggtggctgtc agtgaacagg gcgaggcact gccgcaggcg caagccacgg
      361 caataatggc ggggatccag ccattgcctg aaggtgcggc agaaaaagcc cgcacgcaaa
      421 tcgaatctca attacccgca ggttacaagc cggtttatct taaccagctt caactgttgt
      481 atgccgcacg cgatatgcaa cccatgtggg aaaaccgtga tgctgttaaa gccttccagc
      541 aacagctggc agaggtggcg attgccggtt tccagccgca gtttaataaa tgggtagagt
      601 tactgaccga tcctggtgtt aacgggatgg cacgcgacgt agtgctctct gatgcgatga
      661 tgggctatct ccatttcatt gcaaatattc cggtcaaagg cactcgctgg ctatatagca
      721 gtaaacctta tgcacttgca acgccgccgc tctcggtgat taaccaatgg cagctggcgc
      781 tggataaagg tcaattgcct acgtttgttg caggactggc accgcagcat ccgcaatatg
      841 cggcgatgca tgaatcgtta ctggccttac tcagtgacac caaaccgtgg ccccaactga
      901 ccggcaaagc aacgttgcgc ccagggcagt ggagtaacga cgtaccggcg ttgcgcgaaa
      961 tattgcaacg cacaggcatg ttggacgggg ggccgaaaat tactctacct ggcgatgaca
     1021 cgccaactga cgcggtagtc agcccatccg ctgttactgt tgaaacagca gaaactaagc
     1081 cgatggacaa gcaaacgacg tctcgtagta aacctgcgcc tgccgttcgc gccgcctacg
     1141 ataatgaact ggtggaagcc gttaaacgtt ttcaggcatg gcaaggattg ggggcagatg
     1201 gtgctattgg cccggcaaca cgtgactggt taaacgtaac gcccgcccag cgtgctggcg
     1261 tgttggctct caacatccag cgattgcgct tgctgccaac agagctttct accgggatca
     1321 tggttaacat tccggcctat tcgctggtct actatcagaa cggcaatcag gtgctggatt
     1381 cgcgagtcat tgtcggtcgc cccgatcgca aaacgccgat gatgagcagt gcccttaaca
     1441 acgtagtggt aaacccgccg tggaacgtac ctccaactct ggcacgcaaa gatattctgc
     1501 caaaagtgcg caacgatccg ggatatctcg aaagccatgg ctatacggtg atgcgtggct
     1561 ggaacagcag agaagcgatt gacccatggc aggttgactg gtctacaatc acggcctcga
     1621 atttaccgtt ccgcttccag caggctccag gcccacggaa ctcgctgggg cgctataaat
     1681 tcaatatgcc gagttcagag gccatttatt tgcatgacac gccgaaccac aatctgttca
     1741 agcgtgatac acgcgcattg agctcaggct gtgtacgagt gaataaagct tccgatctgg
     1801 cgaatatgct gttgcaggat gcaggctgga atgacaaacg tatttctgat gcgctgaagc
     1861 agggtgatac acgttacgtc aatattcggc agtcgattcc ggtgaatctc tactacctga
     1921 cggcctttgt tggtgcagat ggtcgtaccc agtatcgtac agatatttac aattatgatc
     1981 tgcctgcgcg atccagctcg caaatcgtat cgaaagcgga acaattaatc aggtaaatga
     2041 agtagttcgg ggaaatcagt tgtcgtaata attagcatga ttgggggcga ttctctgcag
     2101 cccccgtcac tgctggggtt gagtcatctt gacgtctgct ttacgggcgg ttaaggtgcc
     2161 tcttgtgcgc cagaagtgca tataaacgat aacattgacc tgtagacttg attatcatgg
     2221 acaaattcga cgctaatcgc cgcaaattgc tggcgcttgg tggcgttgca ctcggtgccg
     2281 ccatcctgcc gacccctgcg tttgcaacac tctctacccc acgcccgcgc attttgacac
     2341 tcaataatct tcataccgga gagtcaatca aagcggagtt tttcgatggc agaggctata
     2401 ttcaggaaga attggcaaaa cttaaccatt ttttccgcga ttaccgcgcg aacaaaataa
     2461 agtccatcga cccaggatta ttcgaccagt tgtatcgcct gcaagggttg ttaggcacgc
     2521 gcaaaccggt gcaactcatt tccggttatc gttccattga taccaacaat gaactacgcg
     2581 cccgcagccg tggagtagcg aagaaaagct atcacactaa aggccaggcg atggatttcc
     2641 atattgaagg tatcgcgtta agcaatattc gcaaagccgc gttatctatg cgcgcaggtg
     2701 gtgtaggata ttacccacgt agtaactttg tgcatattga taccgggcca gcacggcact
     2761 ggtagcaatc gcttaacgaa acaggggcag tatgaactat cgtattattc cggtcaccgc
     2821 attctcccag aactgttcat taatctggtg tgaacaaacc cgtctggccg cactggtcga
     2881 tcctggcggc gatgcggaaa aaatcaaaca ggaagttgat gacagcggcc tgacactgat
     2941 gcagatcctg ctgacgcatg gtcatctgga ccacgttggc gcagcggcgg aactggcgca
     3001 acattacggc gtgccggttt ttggcccgga aaaagaagat gagttctggc tgcaaggctt
     3061 gcctgcgcaa agtcgtatgt ttggtctgga agagtgccag ccgctgacgc cagatcgttg
     3121 gctgaacgaa ggcgatacca tcagcatagg aaatgtgact ttacaggtgt tacattgccc
     3181 tgggcatacg ccgggtcatg tcgtgttttt tgatgatcgg gcaaagctgc tgatttctgg
     3241 cgatgttatt ttcaaaggcg gagtagggcg cagtgacttc ccgcgtggcg atcataatca
     3301 actgatttct tcaatcaaag ataaattgct gccactgggg gatgacgtga catttattcc
     3361 gggtcacgga ccattatcca cacttggtta tgaacgcctg cataatccct tcctgcaaga
     3421 cgaaatgccc gtctggtaag gcacataaaa aagcccgctt ttaatgcggg cctggatttc
     3481 tggcaaagtg cgctttgttt atgccggatg cggcacgagc gccttatccg gcctacaaaa
     3541 tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcctg ataagcgtag cgcatcaggc
     3601 aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg ggcttcattg tttttaatgc
     3661 ttacagcact gccacaatcg cttcgcacag cggagccatg ttatctggtg tcatcccggc
     3721 cacatttacg cgaccagaag caaccgcata tacgccaaac tcttcgcgca gacgcagcac
     3781 ttgttctttt gtcaggccac tgaaggagaa catgccgttc tgtttgatga taaagctgaa
     3841 gtcgcggttt gcgccttttt cctgcagcgt attgacgaac aactgacgca tacgctgaat
     3901 acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaacgcat cgttgctcag
     3961 gatggtggca acaacagaag cgccgtgtgc tggtgggtta gagtagttag cgcgaatcgc
     4021 cgctttcatt tggctgaatg cgcgatcaac ggtttcactg tcggcagcaa ccagagtaca
     4081 agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg caacaatcag
     4141 ctctttatgc atagccgcga aagcgcgcag tccttcagca tcttcttcca gaccacgggc
     4201 aaaaccctgg taagcgaagt caaacagcgg taaccagcct ttctcaacgg agagttgtgc
     4261 cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc agcagccatg
     4321 gaacagcact acgtcgccag cctgagcttc attcaggctg ttaatcagtg catcgaagtc
     4381 aagagtgtga ttttccgcat cataataagc gtattcacga acttccagac ctgcagagtt
     4441 aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacactcgct taacgctggt
     4501 attttttgcc aggaaatcgg cagctacgcg tagtgcgcca gtgccccccg gagtctgtgc
     4561 catgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca gttcctgagt
     4621 gcagcgacca aattcaggga tgccgtcaat gccgaggtaa tttttggtgg tttcattttc
     4681 gagcagatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc ccgtctcatc
     4741 tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac gaaacagatc
     4801 ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca tgacgaggtt
     4861 ccattatggt tacagaaggg aagtccgcta tcagggtaac gggagattta caaaattcca
     4921 actattactg atgaaaacgc aggctgtttt tgcaagacgt gagattgctc tggaaggtat
     4981 aaaaaaaaca ggaccaaagt cctgtttttt cggcatttaa caaagaggtg tgctattaga
     5041 actggtaaac gatacccaca gcaacggtgt cgtctgaacc tacgcccagt ttgttatcag
     5101 aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg aagtagtagg
     5161 ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct acgtctttcg
     5221 ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg tattgcgcaa
     5281 ctaacagaac gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat ttattagtga
     5341 tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg ttcgcgtcgt
     5401 acttcagacc agtagcccac tgttcagctt ttttaccgtt gccaagaggt tgagcttctt
     5461 gcaggttggt acggtcagct gcaccataag caccaacgat accaaagcct tcgtattcgt
     5521 agctgataga accgccaaca ccgtcgccgt tagaacggcg tgcagtgtca cgctcgtttt
     5581 tacccaggta ctgaacagcg aagttcaggc catcaaccag accaaagaag ttggagttac
     5641 gataggtagc aacgccgcca acacgaccaa cgaagaagtc atcgctgtat gcagtatcac
     5701 caccaaattc tggcagcata tcggtgtaac ccagtgcatc ataaaccaca ccgtagttac
     5761 ggccgtaatc gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc agacgcgttt
     5821 tgttaccagt ttgagcgtca gcgccttcag agttgttacc ttggaagtta tattcccact
     5881 gaccataacc ggtcagatcg gaattgattt gagtttcccc tttaaaacca agatgggcat
     5941 aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt acccttggaa aaataatgca
     6001 gaccaacagc tttaccgtac agatctactt tgttgccatc tttgttatag atttctgcag
     6061 cgtttgcagt acctgctact aacagagcag ggacgatcac tgccagaata ttgcgcttca
     6121 tcattattta ttaccctcat ggtttttttt atgacacctg ccactgccgt caataagttc
     6181 tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc
     6241 atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctgcaaagat aaagatttag
     6301 tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggaactttgt gaacaccgtc
     6361 atatttccat agaaacgtga tgatatttac agcaattttt atctatttat atgatttact
     6421 tatatttaaa ttaactaagc ggaaattttg tttctgatgg aaactttatc gacctggcac
     6481 aaaatcctct ttcagacatc cagaatgcca aaaaatagta tgaaaattgt gctatcagct
     6541 aagaaaaaac taatccgcaa taagaatacg ggttattttt tgtgcgtaaa tgttgcaatg
     6601 taatattccg taacaggatg atcgttatgg cggggttgca aatagattgc ttgacttaac
     6661 aaacagataa cttgacagaa aggataaaaa agagccagca ggcgctggct ctttgaggaa
     6721 gaaaatcaga agctggcgtt gcgcggagta cgtgggaacg gaatcacatc acgcacgttt
     6781 tgcacgccag ttacgtaagc aatcaggcgt tcaaaaccaa gaccgaaacc tgaatgcgga
     6841 acagtaccgt agcgacgcag atcgcgatac caccagtaat cttctttatt caggcccatt
     6901 tccagcatac gctcgtccag aacgtccaga cgttcttcac gctgggagcc tccaatgatc
     6961 tcaccgatgc ccggagccag aacgtccata gccgcaacgg ttttaccgtc ttcgttgagg
     7021 cgcatataga acgctttaat atctttcgga tagtttttaa ccactaccgg tgctttaaag
     7081 tgttcttccg ccagataacg ctcatgctca gaagagagat cgacgcccca gtaaaccggg
     7141 ttttcaaact tcctgccgca gttttcgaga atggtcactg cgtcggtgta atccacctgc
     7201 gcgaaatcgg cctcaatgaa gcgttccaga cgtgaaacgg catctttatc tacgcgttca
     7261 gcgaagaatt tcatgtcgtc tgcgcgttct tcgagaaccg ctttgaagac atatttcagc
     7321 atggcttcag ccagacccgc gatatcgttc aggttagcaa acgccacttc cggctccagc
     7381 atccagaatt ccgccaggtg acggctggtg ttggagtttt cagcacggaa agtcgggccg
     7441 aaggtataaa ttttggataa tgcgcaagcg taggtttcgc cgttcaactg gccagatacg
     7501 gtcaggaaag actctttacc aaagaagtct ttgtcgaaat ccactttgcc ctgatcgtta
     7561 cgcggcaggt tttccagatc cagcgtagaa acgcggaaca tttcgcctgc accttcggta
     7621 tcagatgcgg taatcagtgg cgttgaaacc cagaagaatc cctgctcgtt aaagaagcga
     7681 tgcagcgcct gcgccagcgt atggcgaacg cgcgcgacgg caccaatcag gtttgtgcgc
     7741 ggacgcaggt gagcgacttc acgcagatac tcaatgctgt ggcgttttgc cgccatcggg
     7801 taagtgtctg gatcttcaac ccaaccagca acttcaactt tgctggcctg aatttcaaat
     7861 tgttgcccct ggcccggcga cgccacgact ttacccgtca caatgaccga gcagccggtg
     7921 gtcagacgca ggacgtcttc attgtaattg ggcagagaat tattgatgac agcctgtaca
     7981 ggatcaaagc aggaaccgtc ataaacggcg aggaaggaga tgccagcttt tgaatctcgg
     8041 cgggtacgta cccatccgcg tacggtgact tcgctgtcaa cggctacgcg gccctggagt
     8101 acgtcggcta caggcacaac gctcataata ttctctctgt taatagtcgg aaaaaataaa
     8161 cacttgtcca cccgtaaatg gggggatacc tatgttacct ggcatctgca atcagacaag
     8221 cagaattcgc aaatgcagcg aaagatttcg gaattaaagg taaaaaaagg gagctgatta
     8281 gctccctgat gatattaact ggctttttta atatgcggaa ggtcgaacgc tttgcgcagc
     8341 gcccgaacaa acgctttatc atggcagata gttttgccag ggctgtcaga aagtttcgcc
     8401 accggtttac cgttacactc taccaactta atcacaatat tcaggggttt tacctggggg
     8461 atatcgcagg tcaggcgagt cccaatacca aaacttaatt gcacgcggga agagaagtgg
     8521 cggtatagct caaccgcttt gcgtaaatcc agattgtcag agaaaaccag cgttttactc
     8581 tgtggatcaa ttcccagctt tttataatgt gcaatggctt tttcacccca ttcaaccggg
     8641 tcgccagagt catgacgcag gccctgatac cgactggcga actcgacacc gaaatcacgc
     8701 aggaaagcat ccatagtgat gcagtcggtt aatgcaatgc caagttggtc gggatattct
     8761 tccagccagg cagcaagtgc agctcgctgg ctgttggcta ggtccgggct gatttgctga
     8821 tgtgcctgga accattcgtg cgcctgtgtt cccatcggcg tgagggaaag ccgacgcgcc
     8881 agatcgtagt tgctggtgcc cacgaaccag gattcctgtt gcagacgctt aacgatggtt
     8941 tcttgtactt cgcgagaaaa acggcgacgg gtgccaaaat ccatcagatg gaagcgcgac
     9001 atatcaagac cggcggttaa cgccgagaag tcgactaatt tgctttccag cgtgtcgagg
     9061 gcttgcgcaa cgtcggcctg cggtgagcga tagcgatgta ccatttcact gataaccgcc
     9121 agcaaaggaa cttcccagag gatgacttca cgccacggac cgcttaagcg aatatccagc
     9181 ttgccattat cgttggacac ggtgacttgt tccgggttaa agcggaattc gcgtaaccag
     9241 ttaagatagt cggccttaaa gaaaggcagg gcagagagcc actgatattc atcatcctgc
     9301 aggcgcaggt gctgcatcgc ctgaatctgt tcacgaatag catcggcata aatacccagc
     9361 agatcgtcac ctcggcaacg aaactccgcc gcgacatgca catcgtaata gtgatgaaac
     9421 acggcttgct gcatatgcaa cttataagca tctgtatcca gcaacgagtg cagaacagga
     9481 gaagcgaatt gtgtcatagg tgcgcagtag catcctcgta caggagcgtt tagtacaata
     9541 aacatcttca ggaactgctg gagtatacct tgtttcgcaa tttattgaac cccgatcaca
     9601 ccatatgcca cctttctggt cgatggcatt acgcggtgta tgttataaaa atgtagcaat
     9661 aaaggcgttt gtacctgaaa agatgaagat tctgcatagc gcgatttacg caacaggaat
     9721 agactgaaca ccagactcta taaaagatgc taaaggttat ttatgactca acagccacaa
     9781 gccaaatacc gtcacgatta tcgtgcgccg gattaccaga ttactgatat tgacttgacc
     9841 tttgacctcg acgcgcaaaa gacggtcgtt accgcggtca gccaggctgt ccgtcatggt
     9901 gcatcagatg ctccgcttcg tctcaacggc gaagacctca aactggtttc tgttcatatt
     9961 aatgatgagc cgtggaccgc ctggaaagaa gaagagggcg cactggtcat cagtaatttg
    10021 ccggagcatt ttacgcttaa gatcgttaat gaaataagcc cggcggcgaa taccgcgctg
    10081 gaagggcttt atcagtcagg cgatgcgctt tgcacccagt gtgaagccga aggtttccgc
    10141 catattacgt attatctcga ccgcccggac gtgctggcgc gttttaccac caaaattatt
    10201 gccgataaaa ccaaatatcc cttcctgctt tccaacggta accgcgttgc gcaaggtgaa
    10261 ctggaaaacg gacgccattg ggtacagtgg caggacccgt tcccgaaacc gtgctacctg
    10321 tttgcgctgg tggcaggcga ctttgatgta ctgcgcgaca cctttaccac gcgttctggt
    10381 cgcgaagtgg cgctggagct gtacgtcgat cgcggcaacc ttgatcgcgc gccgtgggcg
    10441 atgacctcgc tgaaaaactc aatgaaatgg gatgaagaac gcttcggcct ggagtatgac
    10501 ctcgacatct atatgatcgt cgcggtggat ttcttcaata tgggcgcaat ggagaataag
    10561 ggtttgaata tctttaactc caaatatgtg ctggcccgca ccgacaccgc cactgacaaa
    10621 gattacctcg atattgaacg cgttatcggc catgaatatt tccataactg gaccggtaac
    10681 cgagtcacct gccgcgactg gttccagctc agcctgaaag aaggtttaac cgtcttccgc
    10741 gatcaggaat tcagctctga ccttggttcc cgtgcagtta accgcatcaa taatgtgcgc
    10801 accatgcgcg gattgcagtt tgcggaagac gccagcccga tggcgcaccc gatccgcccg
    10861 gacatggtca ttgagatgaa caacttctat accctgaccg tttacgagaa gggcgcggaa
    10921 gtgatccgta tgatccacac cctgctgggc gaagaaaact tccagaaagg gatgcaactc
    10981 tatttcgagc gccatgatgg cagcgcggca acctgcgacg actttgtgca ggcgatggaa
    11041 gatgcgtcga atgtcgatct ctcccatttc cgccgttggt acagccagtc cggcacgccg
    11101 attgtgaccg tcaaagacga ctacaatccg gaaaccgagc agtacaccct gaccatcagc
    11161 cagcgcacgc cagccacgcc ggatcaggca gaaaaacagc cgctgcatat tccgtttgcc
    11221 atcgaattgt atgacaacga aggcaaagtg atctcgttgc agaaaggtgg tcatccggtg
    11281 aattccgtgc tgaacgtcac ccaggcggaa cagacctttg tttttgataa tgtctacttc
    11341 cagccggtgc ctgcgctgct gtgcgaattc tctgcgccag tgaaactgga atataagtgg
    11401 agcgatcagc aactgacctt cctgatgcgt catgcgcgta atgatttctc ccgctgggat
    11461 gcggcacaaa gcctgctggc aacctacatc aagctgaacg tcgcccgaca tcagcaaggg
    11521 cagccgctgt ctctgccggt acatgtggct gacgctttcc gcgcagtgct gctcgatgag
    11581 aagattgatc cggcgctggc ggcagaaatc ctgacgctgc cttctgtcaa tgaaatggct
    11641 gaactgttcg atatcatcga cccgattgct attgccgaag tacgcgaagc actcactcgt
    11701 actctggcga ctgaactggc ggatgagctg ctggctattt acaacgcgaa ttaccagagc
    11761 gagtaccgtg ttgagcatga agatattgcg aaacgcactc tgcgtaatgc ctgcctgcgc
    11821 ttccttgctt ttggtgaaac gcatctggct gacgtgctgg tgagcaagca gttccacgaa
    11881 gcgaacaata tgaccgatgc gctggcggcg ctttctgcgg cggttgccgc acagctgcca
    11941 tgccgtgatg cgctgatgca ggaatacgac gataagtggc atcaggacgg tctggtgatg
    12001 gataaatggt ttatcctgca agccaccagc ccggcggcga atgtgctgga gacggtgcgc
    12061 ggtctgttgc agcatcgctc atttaccatg agcaacccga accgtattcg ttcgttgatt
    12121 ggcgcgtttg cgggcagcaa tccggcagcg ttccatgccg aagatgggag tggttaccag
    12181 ttcctggtgg aaatgcttac cgacctcaac agccgtaacc cgcaggtggc ttcacgtctg
    12241 attgaaccgc tgattcgcct gaaacgttat gatgccaaac gtcaggagaa aatgcgcgcg
    12301 gcgctggaac agttgaaagg gctggaaaat ctctctggcg atctgtacga gaagatcacc
    12361 aaagcactgg cttgataaat aaccgaatgg cggcaatagc gccgccattc ggggaattta
    12421 cccctgtttt ctcaggcgaa tttcagattc accacgctgc atcacccttt gcaacacttc
    12481 cgattccagc tccgctaatc gcaccgaccc taatcgacgc ggagggggga tatccactgt
    12541 caaatccaga ccaatttttc cctcttcaat taataacacc cggtcagcca ttgctaccgc
    12601 ttcgctcaca tcatgcgtca ccaacagtac ggtaaagccg tgctcctgcc aaagtgacac
    12661 aatcaaatcc tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag
    12721 caacaataaa cccggtcgat gaatcaacgc tcttgccagc accactcgct gtttctgccc
    12781 gcctgaaagt gcggcaggcc attccccggc gcgattctcc agtcctaccg cagccaacgc
    12841 ccgacgcgcg gcatcgcgcc actggccttt aaggcctaac ccaacgttat caatcaccga
    12901 tttccacggt agcagacgcg catcctgaaa catcattcgc gtgtcttcct gaatttcagc
    12961 cagcggtgtg gtgcctgcca acacatcgcc tgcggttggc gtttccagcc cggccagcag
    13021 gcgcagcagg gtacttttgc caccaccgcc acaaactgac ctgccggaat atgtaaatcc
    13081 agttggttca ggacgatatt ttccgcgtaa tgtttgctta ctgcattgag caacaatggt
    13141 gtgccctggt tcagacgagc agtattcata ccgtggcctc cttcaaatga taagccgggt
    13201 tccagcgcaa ccagaggcgc tctaacagct gcgcgctgac gtcagcaagt ttgccgagca
    13261 gggcgtaaag aataatagcg actaccacca cgtccgtttg caaaaactct cgcgcattca
    13321 tcgccagata accaatgccg gaattggcag aaatggtttc ggcaacaatc agcgtcagcc
    13381 acatcaggcc caacgcaaaa cgcacgccga ccataattga gggcagggca ccaggcagga
    13441 tcacatggat aaacagcggt atgccggata atccatagct acgcgccatc tccaccagcc
    13501 cgcgatcgat attacggatg ccatgccagg tgttgatata aatgggaaac agcgtaccga
    13561 gcgccaccag aaagattttt gcggattcat caatgccaaa ccacaaaatc accagcggga
    13621 tcagcgccag atgcggcacg ttgcgcaaca tctgaattga ggtatccagc agccgttctc
    13681 cccagcgaga tagcccgcta atcagtccca aaatcaaccc cagcgatccg ccaattgaaa
    13741 agccaatcag cgcccgctag gaactgatcg ccagatgctg ccacagttcg ccgctggcgg
    13801 agagcgtcca gaacgccgtc accacccctt ccggtgaggg caaaatacgc gtcgacagcc
    13861 agccaactga ggaggccagt tgccacaccg ccacgatgcc catcggtaaa aactaggggg
    13921 caacgcgcaa taaccacttc ttcactggcg ttgccattat ttcttcctta gctttgcgcg
    13981 actttacggg ggataaaatc attcgccacc gcttcgcctt gcggattcag cggctgcggc
    14041 tggggaattt ccggaatggc gacatccaga tgtgggaaca gcaactcgcc gacccgatac
    14101 gcttcttcca gatgcggata gcccgaaagc acaaagctgt cgatgccaag cgcggcgtat
    14161 tcgttgattc gcgcagcgac cgtaggacca tcgcccacca gcgccgttcc ggcaccgccg
    14221 cgcactaagc caacgcccgc ccataaattg gggctgatct ccagattgtc gcgcttgccg
    14281 ttatgtaacg ccgccattcg ctgttgtcct acggaatccg tccgggcgaa tgcggcctgt
    14341 gctttggcga tagtttcatc atcaagatgc gagattaacc gctcggcggc ctgccacgct
    14401 tcgtcgttag tttcacgaac aatcacatgc agacgaatac cgaaacgaat tttgcgtcca
    14461 tgcgcggcag ctttcgcccg cacttgttcg atcttctctt ttactagttc tggcggttcg
    14521 ccccaggtga ggtagagatc aacttgttct gccgccagct cctgggcgac gtctgacgat
    14581 ccgccaaagt aaagcggagg ataaggctgt tgaatcggcg ggaagagcag ttttgctccg
    14641 cgcacatgaa tatgtttacc gttgaaattg acggtttccc ccagcaataa gcgccgccag
    14701 acctaggtaa attccgccga ggcttcgtag cgctcgctat gatcaaggaa cacgccgtcg
    14761 cctgccagct cttgtggatc gctgcctgtg accagattaa acaacgcacg accatttgag
    14821 agacggtcaa gcgtggcggc ctggcgagcg gcaacggtag gtgaggttac gctggggcgc
    14881 agggcgacaa gaaacttcag ccgctgcgtc accgggatca tcgacgccgc caccagccac
    14941 gcatcttcac acgatcgccc cgttgggatc agcacaccgg tatagcccag acgatccgcc
    15001 gcttgcgcaa tttgttgcag ataaccgtga tcaaccgggc gtgaaccttc ttccgttccc
    15061 agatagtgcc cgtcaccgtg ggtcggtaaa aaccagaaca tattcagact cataattgtt
    15121 ttccttccag ttgagtgggc tgccagatgc gctggcgaat atcgactttt ttcggcacca
    15181 gacgattttc ataaaacaga tctgccgttt gctgctgtaa ggcggcaacc tcggcgttaa
    15241 ccggtttgat ggtggtagga gggcgatgat ctaagtaaga ggcaatcacc ggtgccggta
    15301 agcccatcgt ttttgccagt aaagcgatgc tttgctcgcg ctggctgcgg gttaacgcat
    15361 cggcctcact aaaggttgcc agtacgccct gaataaaagc gccgtttttt tctgcatagg
    15421 gacgagctgc cagataaaac gatccagttt gattgagatc ggtgccgtct ttcagcaccc
    15481 gcacgccgcc ctgtaataat gcagcagagt agtagggatc ccagatagcc caggcgtcaa
    15541 cgttaccttg ctggaacgcg gcgcgggcat cagcgggcgt caggtaagtg ggttggatat
    15601 cggtaaactt aagtccggcc tgacgcagtg cacgcagtaa aaggttgtgt gaactggaac
    15661 ctttctgaaa ggcaactttg tgacctttaa gatcggctac ggttttgatc gggctgtttt
    15721 ctgccaccag aatcacttcg gctttgggct tcggtggctc gacgcccaca tataccaaat
    15781 cagccccggc agcctgggca aagattggcg gaatatcccc ggtactgccg agatcaatac
    15841 tgccaacgtt taaggcttcc aacatttgcg gacccgcggg gaactccacc caggagattt
    15901 ttgattgcgg atagcgtttt tccagtaact ggtggctttt tgccagtacc ataccaatac
    15961 tgcctttctg atagcctata cataacgctt caggcgagga ttctgcagca accgcaatcg
    16021 tagagacgct aagcaatccc accagcgcca gtttaatgat gttacgcatg ggcattacct
    16081 cgcagagaca gaaggtcagg aacctgaaca tcgcggcggt gcaatgcctg ccagaaagtt
    16141 tctagcgcgg tatcaagacg ggtttgcaga tttggcgtga actggggtct gtgatggtaa
    16201 tcaattactt gtgagtcatc ggcaaacacg ccgtgcagga tctcctgagc tttcagtgcg
    16261 cttaaaactg gtttaagggc ataatcgacc gccagcagat gggccacggt accgcccgtc
    16321 gccagcggta gcaccacttt gccttgcaaa gcgcgctctg gcagcaggtc gagcagggtt
    16381 ttcagcgcgc cggaataggc ggctttatac acaggtgtag cgactatcag tccatcagcc
    16441 tgttgcagct gttcggtgaa ggtcttgagt gccggactat cgaaacgagc ataaagcaga
    16501 tcttccggga cgaagttttg cagattccag tgataaacct ctacatccag gccatttagt
    16561 ttttcccgcg catattccag caaggagctg gagcgagaag gaaagcgagg actacccgcc
    16621 agggtgatga cacgcatact ctctccttat aaccaattgt tctttttttg ttaacattga
    16681 taacaattcg gtcagtctgt cggagagaca agaaaatgcc aaatataaat tttgtgtatc
    16741 tttttctgaa aataaactaa agaaagggct ttatatatca cgcatattta tttgttggat
    16801 agtcattaga tgttgaatca atgaattact cgttcagtta atatttaaat attcatgaaa
    16861 tctataaatt aaagatttgt cacttattgg atttagtatg cttgatttaa atgacggtca
    16921 attattgtta ttattaagtc taatgtcatt taaaggcatt atcatattta cgaagagatc
    16981 aggatgatga cgatgaaaaa aagtgtattg acggcgttta taactgtggt atgtgcaacg
    17041 tccagcgtta tggcagctga tgataatgct atcacggatg gcaaagtaac atttaatggt
    17101 aaagttattg ctccagcttg taccctggta gctgcgacga aagattcagt agtcacattg
    17161 ccaaatgtca gcgccaccaa gttgcaaact aatggagcag tctctggcgt taaaactgat
    17221 gtaccaattg ccttagaagg ctgtgatgtt actgtaacta aaaatgcgac gtttaccttt
    17281 agtggtacgg ctgacggcgt gcaacctaca gcatttgcca accaggctac caccgatgca
    17341 g
//
LOCUS       DAJTSL010000079        17325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTSL010000079 DAJTSL010000000
VERSION     DAJTSL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.079"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..693)
                     /gene="rlmB"
                     /locus_tag="OO433_002374"
     CDS             complement(<1..693)
                     /gene="rlmB"
                     /locus_tag="OO433_002374"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6050823.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     AQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLVRT
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVAT"
     gene            complement(874..3315)
                     /gene="rnr"
                     /locus_tag="OO433_002375"
     CDS             complement(874..3315)
                     /gene="rnr"
                     /locus_tag="OO433_002375"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6050824.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAECRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRLVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3354..3779)
                     /gene="nsrR"
                     /locus_tag="OO433_002376"
     CDS             complement(3354..3779)
                     /gene="nsrR"
                     /locus_tag="OO433_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6050825.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3984..5282)
                     /gene="purA"
                     /locus_tag="OO433_002377"
     CDS             complement(3984..5282)
                     /gene="purA"
                     /locus_tag="OO433_002377"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6050826.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5386..5583)
                     /locus_tag="OO433_002378"
     CDS             complement(5386..5583)
                     /locus_tag="OO433_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6050827.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5665..6669)
                     /gene="hflC"
                     /locus_tag="OO433_002379"
     CDS             complement(5665..6669)
                     /gene="hflC"
                     /locus_tag="OO433_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6050828.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6672..7931)
                     /gene="hflK"
                     /locus_tag="OO433_002380"
     CDS             complement(6672..7931)
                     /gene="hflK"
                     /locus_tag="OO433_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6050829.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTYPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8017..9297)
                     /gene="hflX"
                     /locus_tag="OO433_002381"
     CDS             complement(8017..9297)
                     /gene="hflX"
                     /locus_tag="OO433_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6050830.1"
                     /translation="MFDRYDAGEQAVLVHIYFTKDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETILADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9374..9682)
                     /gene="hfq"
                     /locus_tag="OO433_002382"
     CDS             complement(9374..9682)
                     /gene="hfq"
                     /locus_tag="OO433_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6050831.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9768..10718)
                     /gene="miaA"
                     /locus_tag="OO433_002383"
     CDS             complement(9768..10718)
                     /gene="miaA"
                     /locus_tag="OO433_002383"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6050832.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAITG"
     gene            complement(10711..12558)
                     /gene="mutL"
                     /locus_tag="OO433_002384"
     CDS             complement(10711..12558)
                     /gene="mutL"
                     /locus_tag="OO433_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6050833.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12568..13905)
                     /gene="amiB"
                     /locus_tag="OO433_002385"
     CDS             complement(12568..13905)
                     /gene="amiB"
                     /locus_tag="OO433_002385"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6050834.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKHSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13924..14385)
                     /gene="tsaE"
                     /locus_tag="OO433_002386"
     CDS             complement(13924..14385)
                     /gene="tsaE"
                     /locus_tag="OO433_002386"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6050835.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14357..15904)
                     /gene="nnr"
                     /locus_tag="OO433_002387"
     CDS             complement(14357..15904)
                     /gene="nnr"
                     /locus_tag="OO433_002387"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6050836.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLFAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHSITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWGDVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15903..17042
                     /gene="queG"
                     /locus_tag="OO433_002388"
     CDS             15903..17042
                     /gene="queG"
                     /locus_tag="OO433_002388"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6050837.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQPICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGGH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17025..17078)
                     /locus_tag="OO433_002389"
     CDS             complement(17025..17078)
                     /locus_tag="OO433_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050838.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3943 a         4945 c         4340 g         4097 t
ORIGIN      
        1 ggttgcaacc gaaacgttca gagaagaaac acttcctgcc attgggatgc tgatcaactc
       61 atcgcaatgt tcacgggtca ggcgacgcat accttcacct tccgcgccca tcaccagcgc
      121 caggcgtccg gtcattttgc tctgatagag tgtatgatcc gcctcacctg ccgtaccgac
      181 gatccagata ttctcttcct gcaacatacg catggtacgc accaggttag tcacccgaat
      241 cagcggaacg ctttctgccg cgccacaggc cacttttttc gccgtggcgt tgagctgggc
      301 ggagcgatct ttcggcacaa tcaccgcatg aacaccagcg gcatctgcgc tacgcaggca
      361 cgcgccgagg ttatgcggat cggttacacc gtcaaggatc agcaggaacg gctgatcgag
      421 cgaagcgatc agatccggca aatcattttc ctggtactga cgtcctggct tcacgcgggc
      481 gataatgccc tgatgcacgg caccgtcgct tttctcgtcg agatattggc ggtttgccag
      541 ctggataacc acgccctggg cttcgagggc gtgaatcagc ggtaacagac gtttatcttc
      601 acggcctttt aaaataaaga cttcctgaaa acgttcaggg gcgcgctcca gcagggcctg
      661 cactgcgtgg atgccgtaaa tcatttcgct cattaatgta ctcgttgttt acgttttgcc
      721 tgatgcacta cgtttatccg gcctacatga tctctgcaat atattgaatt tgcgtgatta
      781 tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa caaagcgcat tttgtcagca
      841 atctaaccct cttctgtttg aagagggtaa tgatcactct gccacttttt tcttcgccgc
      901 acgcttcgct ttggtcgctg cagctatttt ctgcgttttc gccgatggct ttttcgcttt
      961 tctcgcgtct ttcttcgctg ctttcggctt cgtttttttc tcaccgcgga aggcgctgtc
     1021 tggctcaaag tttacctttt taccgaccag acgacgcttg ccgccttttt tacctgcatc
     1081 gccttttttc gctttctcgc gcgccgtttt accgacgttg cgcggtgcgc gttcgctgga
     1141 gatcaggcta aagtcgattt tgcgctcgtc catattaacc gcttcgacgc gaacttccac
     1201 gcgatcgccc agacgataag tctggccgct ggattcaccc atcagccgtt gccctacctg
     1261 gtcaaagcga tagtagtcat tgtccagcga agagacatgg accagaccat caatgaataa
     1321 gtcgtccaga cggacaaaga atccaaagcc agtgacgctg gaaattacgc ctttaaagac
     1381 gttacctacc tggtcgagca tgaagtcaca cttcagccag tcagccacat cgcgcgttgc
     1441 ttcgtcggca cgacgttccg ccatcgaaca gtgctgaccc agttgcagca tttcttccat
     1501 cgaataatgg tagccgccgg tttcagtggt gttgccctga tgcccctgct ctttcgccag
     1561 cagatattta atggcgcggt gcagcgtcag gtctggataa cgacgaatcg gcgaagtaaa
     1621 gtgcgcatag gactgcaatg ccaggccaaa gtgaccacgg ttttctggat cgtaaatcgc
     1681 ctgtttcatc gagcgcagca gcatggtttg cagcatttct gcatcaggac gatcggcaac
     1741 cgactccagc agctccgcgt agtcacgcgg ttccggcttg ttaccgcccg gcagttccag
     1801 ccccagctcc gccagtactg aacggaaaga ggtaatcgct tcggtgctcg gcttgtcgtg
     1861 aatacggaac aatgctggtt ctttggcttt ctcaacgaaa cgcgccgccg agatattcgc
     1921 cagaatcatg cactcttcaa ttaacttgtg tgcgtcgtta cgctgagtct gttcgatacg
     1981 ctcaatacgg cattcagcgt tgaaaataaa cttcgcttct tcgctctcaa atgagatccc
     2041 gccacgttct tcacgggctt tatccagcac tttatagagg ttatgcaact cttcgagatg
     2101 cttaaccagc ggagcgtact gctcgcgcag atcctgatcg ccctgcagaa tatgccagac
     2161 tttggtgtag gtcagacgcg cgtgagagct catcaccgct tcgtagaatt tgtagcccgt
     2221 caggcggcct ttcgacgaaa ccgtcatctc gcacaccata cacaggcggt ctacctgcgg
     2281 attgagcgag cacagaccgt tagagagcac ttccggcagc atagggataa cctgcgaagg
     2341 gaagtacacc gacgtaccac gattacgcgc ttctctatcc agcggcgttg gcggacgcac
     2401 atagtagctg acgtcggcaa tcgcgaccca taaacgccag ccgccgccgc gttttttctc
     2461 gcagtaaacc gcatcgtcaa agtcacgggc gtcttcgcca tcaatggtga ccagcggtaa
     2521 atcgcgcaaa tcgacacggc ccgcttttgc ttcttccggc acttcttctt tcagcccggc
     2581 aacctgttgc tcaacagcct gcggccagat gtacggaatt tcatgggtac gcaaagcgat
     2641 atcaaccgcc atgccggtgc ccatattgtc gcccagcact tcgacgattt tacccaccgc
     2701 tttggtacgg cgagtcgggc gttgggtcag ttcaacaacc accacaaagc ccatccgcgc
     2761 gcccatgatc tgatcgggcg ggattaagat atcgaagctc agacggctat cgtcaggaac
     2821 cacaaagccg acgcccgctt cggtaaagta gcgaccaaca atctggctgg tttttggcac
     2881 cagtacgcgg acaatacgcg cttcacgacg acctttacgg tcagcaccca gcggctgagc
     2941 cagcacctga tcgccatgaa tgcaggtttt catctgctcg ctggagagat acaaatcatc
     3001 tttacgccct tcaacccgca gaaagccgta gccatcacgg tggccaataa cggtaccttt
     3061 caccaggtcg aggcgttccg gcagcgcata gcactgacga cgagtgaaga ccagttgacc
     3121 atcgcgctcc atcgcgcgca ggcggcgacg caggccttca agctgctctt cgccttcaat
     3181 gtgcagttct accgccagct catcacggct ggccggtttt tcacgtttgg ttaaatgttc
     3241 gaggataaat tcccgactag ggatgggatt cgcgtatttt tcagcttcgc gttcctggaa
     3301 aggatcttgt gacatctcgg ttcctccgtt gtcatctctg atgaagattt tcgtcactcc
     3361 accagcaata atttataaag cggttgattc tcttcaacca aatcggcaag cgtgtagtta
     3421 tccagttccg taagaaaact ttgcacggcc ttagaaagcg cctgtttcaa ccgacaggca
     3481 ggtgtaatgt ggcaaaactc actgctgcaa ttcaccagcg ataagggctc cagctcgcgc
     3541 accacatcac caatacgtat cgcactcgcc ggtttaccca ggcgaatgcc gccatttttt
     3601 ccacgaacag cagtcacgta gccggcacga ctaagttgat tgattatttt gaccatatga
     3661 ttacgggaga cgccgtagac gtcagtcact tcagaaatac tggtcatccg cccttctggc
     3721 aatgacgcca tgtagatcag cgcacgtaat ccgtaatcag tgaaactcgt taactgcaca
     3781 tcaacctcaa aagggaaatc gggaaaaaat acatttatat tgatgataaa ccagccacaa
     3841 gctgtgtcgc taatttattt cagttcgggg aaggaaaaaa gcgaggattt aacactgtgt
     3901 cggatagcgt aatggcttaa ccgacacagc aatgtatcac actgttagcc cggcaggcaa
     3961 aatatctgcc aggcgtacca gaattacgcg tcgaacgggt cgcgcagaat catagtttca
     4021 gtacgatccg gaccggtaga gatgatatcg atcggcacac cggtcagctc ttcaatacgc
     4081 ttgatgtagt tcagcgccgc ctgcggcagc ccgctacgat ctttcacgcc gaaagtggat
     4141 tcagaccagc ccggcatggt ttcgtaaatc ggctctacac ctttccagtc gtcagctgcc
     4201 agcggagtgg tagtcacttc gcgaccatcc ggcatacggt aagccacgca gagtttaacc
     4261 tctttcaggc catccagaac gtccagttta gtcaggcaga agccagacag ggagttcagc
     4321 tgtaccgcac gacgaacggc aacggtgtcc agccagccgg tacgacgacg acgacccgtc
     4381 gttgcgccga attcgttacc ctgcttgcag aggaactcgc cagtttcatc aaacagttcg
     4441 gtcgggaaag gacctgcacc cacacgagtg gagtaagctt tgaggatacc cagaacgtaa
     4501 tcaacataac gcgggcccag gccggaaccg gtcgccacgc caccagcagt ggtgttggaa
     4561 gaagttacgt acggataagt accgtggtcg atatccagca gcgtaccctg cgcaccttca
     4621 aacatgacga aatcgccacg ctgacgcgcc tggtcgagca ggtcagaaac gtcaaccacc
     4681 atagaagtca ggatgtcggc aacagccatc gtatcatcca gaactttctg gtaatcaacc
     4741 gcttcagctt tgtagtagtt aaccaactgg aagttgtgat attccatcac ttctttcagt
     4801 ttttcagcga aggtttcttt gtcgaaaagg tcgccaacac gcagaccgcg acgtgccact
     4861 ttatcttcat aagcaggccc gataccacga ccggtggtgc cgatcgcttt cgcgccacgc
     4921 gctttctcac gcgcgttatc cagcgcaacg tgataatcaa ggatcagcgg acatgcttca
     4981 gacagcagca gacgctcacg aacggggatg ccacggtctt ccagttcttt catctctttc
     5041 atcagcgcgg ccggagacag cacaacaccg ttaccgatga tgctggttac attctcgcgg
     5101 agaatacctg atggaataag atggagaacg gttttttcac cgttgattac gagagtatgg
     5161 cctgcgttgt gaccgccctg gtagcgtaca acatatttag cccgttcagt cagaagatcg
     5221 acgatcttac ctttaccttc gtcaccccat tgggtgccca gtacgacgac gttgttaccc
     5281 atttttcaaa atcaccgttt gcttaaaaat ggattctacc atcgcttttt cagagttaca
     5341 gcactttttg cactcaaaaa tgacctcgat cagtctgttt tttgttcagc caatcgtttt
     5401 cctcaacatg tagtagacca caacgcccgc aaccacaagt ccaccgccaa aacgacgtaa
     5461 aatattatcg ggcaaattgg tcatcgcaga gatcatcttc ttccatgcct tcgggtaaag
     5521 catcggccct aaaccttcca gtaccaaaac cagggcaagc gccagccaga ttgtcgaatt
     5581 cattcattat ccttatagaa aaagaaaacc accgacatcc ctgaggatgc ggtggcttta
     5641 ttgacctgta ccgcagtcgt tatattaacg cgttgcggaa gtcggcgtct tcatgtagcg
     5701 gaagaaatcg ctatccgggc tcatgaccat cacgtcctga ttgccagaga agctgttctc
     5761 ataagcacgc aggctacgga tgaatgcgta gaagtccgga tctttactga atgcatcagc
     5821 aaacagtttg gctgcttcgg catcgccttc accacgcatg atgcggccct gacgctcagc
     5881 ttctgccagc gttctggtca cttcatagtc ggcagtcgcg cgcagttttt ccgcttcttc
     5941 ctgaccttgt gaacggtgac gacgcgctac cgcttcacgc tcggcgcgca tacggttgta
     6001 gatcgcttca gacacttcgg tcggcaggtt gatctgcttg atacgcacat cgacaacttc
     6061 aatacccagc gccgccatac tgttcgggtt gatgaccgga actttgccct tcgtctcagc
     6121 cgttacgcgc tctgccgctt cggcaatggc gttatctgct gccggggtag taacttcatc
     6181 ttctgtaccc gcagaaccgg agttcagcgc gtcacgtact tcgagggtca gacgaccacg
     6241 ggaatcagtg acgatatctt tcacgtccag gcgaccaatt tcagaacgca gacggtcaga
     6301 gaacttacgt ttcaacagca cttccgcttg cgaaatgtcg ccaccacccg ttgccaggta
     6361 gtaacggctg aaatcgctga tgcgccattt gatgtaagag tcgacgatca ggtctttctt
     6421 ctctttggtc acaaagcggt cggcctggtt gtccatggtc tgaatacgtg cgtcgagcat
     6481 tttcaccgtt tcaatgaacg gtatcttgaa atgcagaccc ggctcataaa ccagaggttt
     6541 gttgtcatcg tcacgcagta ccttaccaaa acgcagcgta ataccgcgct caccttcttt
     6601 gacgacaaag acagacatgt aaagcactac cagcacgatg atgataatcg cgataactga
     6661 cttacgcatc gttattcccc ctgacgctgg tagtcgttac gctgcgcgtt ggcgcggcgt
     6721 tggtccataa tatcgccctg actggtggac gacgtgttgc ttgctccact ggttgtggag
     6781 gaagaggctg gcggcaaacg cagcagattg ctggcaccgt tatcgctctt cgccgcaggg
     6841 gcgttaccac ctttcagcat ctggtctaac ggcagaacca tcaggttgcc acctttatcg
     6901 ttaaccagca ctttgcgggt gttacccaac actttttcca tcgtctcgat atacagacgc
     6961 tcgcgagtaa tttccggcgc ggctttatat tccggcagaa gtttagcaaa gcgcgccact
     7021 tcaccctgag cttccaggat ggtctgggcc ttgtacgcac gcgcctcttc gaggatacgt
     7081 tgcgcctgac cgttcgcacg cggctgaact tcgttggtat acgcttctgc ttcacgaatg
     7141 tattgctgtt cgttttcacg cgcggcaatc gcatcgtcaa acgccgcttt tacttcttcc
     7201 ggcggacgag cagcctggaa gttgacgtcc agcagagtga tgcccatgtc atacggacga
     7261 atcgtctctt ccagttcgcg ctgggtatcg ctacgaatca cggtacgacc ttccgtcaga
     7321 atgcggtcca tggtgtattt accgataacc ccgcgcaggg cgctgtcggt agcctgacgc
     7381 aggctgtcat ccgggctggt cacgctatac agatattttt ccggataggt aacgcggtac
     7441 tgcacgttca tctcaacgcg cactacgttc tcgtccgacg tcagcatcac accggaagcg
     7501 gccagttcac gcacggcttc cacgtttacc ggtttgactt cgtcgataaa cgtcggtttc
     7561 cagttcagac ccggctcaac cagatggctg aatttaccaa agcgtgttac cacgccgcgt
     7621 tcggcttctt taatggtata gaaaccactg gccgcccaga taatgacaat cgctgccgct
     7681 gcgatggtaa cgacacgacc gccaagctgc gggcgcgggc cttgcgatga actgccaccg
     7741 ccagatccgg tgcctttacc gccgcccaga ccaccgagct ttttgctcag tttgcggaag
     7801 atatcatcta aatcaggtgg cccttgatcg cgaccgcctt tgtttccatt tccctcagag
     7861 ttgccgccag gtttgctgct tccccacggg tcgcggtctt gtccgttatt accgggctga
     7921 ttccacgcca tgtttgtgct ccatatttgt tatgcggtga tccctgttgt ctttcgacgc
     7981 cagaggatat gactccacgc ttcagacgct acgccgttag atcaggtaat cgatcaacgc
     8041 cggttcttgt ttacagaggc gacgccagtc aacgatcggc atacgaactt gcagacttac
     8101 gctgccgtcc tcctccatcc actctttttc tattgcctga agctgataaa aacgacttct
     8161 cagacgccct tcctgcggtg gcagacgcaa tgtatgctgc gccacctcgc cggaaagccg
     8221 ctccgtcaaa gcctgaaaaa gctgtggtat ccccgctccg gtctgtgcgg aaagccagac
     8281 acggatcggt ttgttctctt cgtcccgatc aatacgcggt tcgaaatctt ccagcatatc
     8341 gatcttgttc atcaccagca gggttgggat ctcgtgagcg tcgatctctt caagaaccgt
     8401 attcaccgct tcgatgtttt cttgtacacg cacatccgcc gcatcaatga cgtgcagcag
     8461 taatgtggct tgccgcgtct cttgtaacgt ggctttaaat gccgccacca gatcgtgcgg
     8521 cagatggcga ataaacccta cggtatctgc aagtatggtt tcaccgacat ccgcgacgtc
     8581 aatacgccgc aacgtcgggt cgagggtggc aaacaactgg tctgccgcgt agacccgcgc
     8641 ttcggtgatg cgattgaaaa gggtagattt accggcgttg gtatatccca ccagcgaaac
     8701 agtaggaacg tcggctttga tacgcgattg ccgcccctgc tcacgctgct tttcaactct
     8761 ttccaggcgc gactgtatct gcacgatgcg attacgcaac aaacgacggt cggtttcgag
     8821 ctgggtttca cccggaccac gcaaacctat cccgcctttc tgtctttcaa ggtgggtcca
     8881 gccacgcacc aggcgcgtag ccagatggcg cagctgcgcc agctcaacct gcaacttacc
     8941 ctcatgggta cgcgcacgtt gggcgaaaat atctaaaata aggccggtgc ggtcgatgac
     9001 acgacactcg cacaaacgct ccaggttacg ctcttgcgcc gggctcaggg catggtcaaa
     9061 aagaacgacc gaagcacccg tcgctttgac agcttccgca atttcaactg ctttaccttc
     9121 acctacaaaa tactttgggt gcggcgcttt acggctaccg gtaatcacct gcaatgcttc
     9181 gacaccggcg gaagaaacca gagattcaaa ctcctggagg tcttccatat ctttgtcttt
     9241 cgtaaaatag atgtgtacca gtaccgcctg ctcaccagca tcataacggt caaacaagcg
     9301 tataaccctc taaatagatc agcggggaac gcaggatcgc tggctccccg tgtaaaaaaa
     9361 acagcccgaa accttattcg gtttcttcgc tgtcctgttg cgcggaagta ttctgcgcgc
     9421 tgctaccatg atggtagtta ctgctggtac cgccaccggc gttgttactg tgatgagaaa
     9481 ccggacgaga cgggacaaca gtagaaatcg cgtgcttgta aaccatctgg ctgaccgtgt
     9541 ttttcaacag gatcacgaac tgatcaaaag actcgatttg cccttgcagc ttaataccat
     9601 tcaccaaata aatagaaact ggaacacgtt cccgacgcag tgcgttcagg aacggatctt
     9661 gtaaagattg ccccttagcc attctctctt ttccttatat gcttatttgt actttgaacc
     9721 tttcgattct gaaaaaattg cgcacgatac gtctcaattg tacacattca gcctgtgata
     9781 gcaccaacaa cctgtaatac ttcgtcacgc gcctgttctg gtttttcact gtcaagccag
     9841 tgaacccctt cccaaccacg cagccaggtt atctgccgct tcgccaactg tctcgtggcg
     9901 caaacacctc gataaaccat ttcatcgtat gagatttcgc cttcaaggta agaccacatc
     9961 tggcgataac ccacgcaacg aatggaaggc aagtccgtat gcaaatctcc tcgggcaaaa
    10021 agcgcccgga cttctgcttc aaaacctgaa gccaacatct gatgaaaacg ctgctcaatg
    10081 cgttgatgga gcagttcacg gctcgccggg gcgatggcga actgatgcac ctgatacggt
    10141 agagcgtctc ctgacgtttg cgtcagttcc gttaaagttt tacccgaaat gaaaaaaact
    10201 tccagtgccc gggaaagcct ttgtggatca tttggatgaa tccttgctgc cgcaaccgga
    10261 tctacctcct gaagttgacg atgcaatgac tcccaacctt gctctgccgc ctgttgctca
    10321 attctggccc gcacttccgg gtctgccgac ggtagcggcg acaacccttc cagcaatgcc
    10381 ttgaaataca acatcgtacc gcccactaac agtgggatcc gccccgccgc ggtgatatcg
    10441 gccatttccg ccagcgcatc gcggcgaaaa tcagcagccg agtaagcctg cgacggatcg
    10501 cgaatatcca gcaatcggtg cggcgcggcg agtaactctt cagcgttcgg cttcgccgtc
    10561 ccgatatcca tccctttgta aataagggca gaatcaacgc ttatcaactc tactggtaaa
    10621 attttacgca gctcaatggc taacgccgtt ttaccggagg ccgtcggccc catcaaaaaa
    10681 atcgccttag gcaggctcgc cttactgata tcactcatct ttcagggctt ttatcgccgg
    10741 atgtaaatca acagattgta acagaccacc cggcggcgtt ttcacaagtt gcggacataa
    10801 ccgttccacg tccgccagca gggttatggc ctgtgccatt gaccactgcg catgttcgct
    10861 catcagattt cgtgcaatcc actgcgcaat attgccaggt tcgaatacgg actgcttcgc
    10921 caggtagcct atcagttcag gaatcaagat ttgtaaattt tgttggcgta agggtaaagg
    10981 cacggccctg atggtcacat gctgtgcatc tgactggaaa tcaataccca attccgccag
    11041 ggcagactgc gctttttcta atgccgattt ttcttcgcca gaaactttta gccgcaacgg
    11101 aataagcagc ggctgagcgc aaactggcgc ttcacccggg gtcagttgta cctgacgcag
    11161 ccaacgttct gccactggca aggctaaaag tgaaatgttg ccgtcgcgct ccagcaacgc
    11221 acagtcggaa tggacgatag tcagtacccg accaaaactc tgactgttcg ccgcaagtgc
    11281 aggttcctgc ggttccggcg cttttaattt ttgcatcggc gcgggcgttt gcaaaagctg
    11341 gcgatacact tcaccttgct gtttctggta gcctggctgc gcattcggcc aaggggcagc
    11401 cggacgactg cctgatgctg gcgcaggagt gtagcgcgga gctaccggct cacgagctgc
    11461 cggttctgca aagtgattgc gccccgccgc cacgcggttt tccggaatgg aacgcggtgc
    11521 aggttggggt tcatcgtcca gcggtagcgg cgtttccagt tgctgttgta gcacgctcag
    11581 cacgccctga tagataaaat catgcaccag acgcgactga tggaaacgca cttcgtgttt
    11641 ggcggggtgc acgttgacgt ccacctgatg cggatcgatc tccagataca gcacaaatgc
    11701 cggttgctga tcggccccca gtttgtcttc gcaggcctgg cggatcgcgt gattgatcag
    11761 gcgatcgcgc atcatgcgac cgttcacgta gcaatactga atttctgcca gtgcgggcgt
    11821 ggtgtgattt ggatcggcca cccagccgcg tagcgtgaga tcgccgtgtt gccattcaat
    11881 cgccagcgct tgttcaagaa aagcggtgcc gcaaatcgcg cctaagcgcc gttctttttg
    11941 cccgccttcc ggcactgcgc ggtactgacg cacaatttta ccgttatgcg acaggttgat
    12001 cgtgacgtcg aaacgcgcca gcgcaatgcg gcggatgatc tcatcaatgt ggttaaattc
    12061 ggttttctcg gtgcgcagga atttgcgccg cgccggggtg ttgtagaaca gatccagcac
    12121 ctccagcgtc gtccccacag gatgcgccgc cggttttacc gtcacgttca tatcgcgccc
    12181 ttcggcatag gcctgccagg cttcctgctg ttctgcggtg cgtgaagtga gcgtcaggcg
    12241 ggaaaccgaa ctgatactcg ccagcgcctc accgcgaaag cccaggctga taatggcttc
    12301 gagatcgtcc agagaggcga ttttactggt ggcatgacga gccagcgcca gcgccagctc
    12361 atcttttttg ataccgcagc cgttatcacg aatgcggata agtttcgccc caccgcgttc
    12421 gatatcaata tcgatacgcg tcgcacctgc atcgaggctg ttttccacca gttctttgac
    12481 taccgacgca ggtcgctcga ccacctcacc tgcggcaatc tggttcgcca gttgtggcgg
    12541 taagacctga attggcatca atcgtcctta gtttggcagc gtgcgatctg gcgtcgtcac
    12601 cgtactggcc gtttgtgccg ttgcaccctg cggcgcagat tgcatcggat gcgcaaggaa
    12661 ataattgcgc aggcctttgt aaatggcttc tgccagctgt tgttggtaat cgtcgctcgc
    12721 cagcaaacgt tcttcgctgt tgttgctgat aaaaccggtt tcgaccagta ctgacgggat
    12781 atccggcgaa cgcagaacgc cgagactcgc gtgttctggt cggcgtttat gtatttcgcc
    12841 aatgcgttgc aactgactga tcatactggt cgctacatca taccctaccc gctgggaatg
    12901 accgaactgt aaatccagca ccgcctggct taaataggga tcagactgac tgttcgccag
    12961 cacatcgccc gccccaccca gcagctccga atgtttctcg tgctgctcca gccagctggc
    13021 catttcgctg ttggcgcgac ggttagagag cacccatacg gaagcgccag tcgcactgcg
    13081 gttcggtgcg gcatcagcgt gaatcgacac gaggaaatta gcgttttgct tacgtgccac
    13141 atcgctgcgc cccatcaccg aaataaagta atccccgtca cgggttaaaa cgcctttaaa
    13201 catcggatcg tcattgagca aagtacgcaa tttacgcgcg atggcgatgg tgacattttt
    13261 ctcccgcgta ccaccggggc cgatagcgcc agggtcctga ccgccgtgtc cggcatcaat
    13321 agcgatgata attttatcgc cagtgttagc cgtcgcgcgc gctgccggac gcgttaccgt
    13381 attactgctg ataacacccg tagtgcggtt actttccgtt ttaaacggat tgcgcgccgg
    13441 ttcgctgacg cgcggtgcga caaccgcagg cgtttcaacg cgtttcgcaa ccacgggcgg
    13501 cggaggaggc ggtggcggca catcggcgtt aatcgtaaag acgacagtgt aattgctgcc
    13561 attctgccgc ttcaccgctt cggttttacc attttcggta agatcgacca ccagccgtag
    13621 cgtttgtgca tctttaggcg ttccagagcg aatcgccttc accagattat tgccgctgaa
    13681 caacaacggc agtccctgaa tcacgcccgt ttgtttgata tcgagcgcca cggtgcgttt
    13741 gctttgatgg ctaaacgcat aatcaggatc gccaataaaa ctcaacgtta tccgcgcctg
    13801 ttgattaccg ttagaaacct gaatatcaga gagcgtcgcg gcacccaccg gcgtgcacag
    13861 cagcagcagc gtcgctacca accaatttct gatgcgatac atcatcccgc cacctttcaa
    13921 aggttaaccg gctaaacgcg ccagcaacaa ttcacccgca gaggaaaccg cactcacgcg
    13981 cgcctcacgg ccttgtgcct gataatcaat gtgtatttcg acatccgggt caggaagaac
    14041 acctgtacct tgttgtggcc actccaccag gcagatggca tcgttggcaa aataatcgcg
    14101 gatccccata aactccagct cctcgggatc ggcaaggcgg tacaaatcaa agtgataaac
    14161 cattaaattg tcgagcgtat agggttcgac cagcgtataa gtggggcttt tgacattacc
    14221 ctgatgaccc agagcttgta aaaagccccg gctaaaggtg gtcttacctg cgcctaaatc
    14281 gccatacaga tagattacgg ttgcgccatc gcaggctttc gctacccgct cgcccaggtc
    14341 taatgttgcc tgctcatcag ggagcggaat tactcgattc atcatggttt ttatcagtca
    14401 cttccgggtt aacaatacgc tgtagcgtgg aaaagagatc ggttgccagc atcccgcgcg
    14461 ttccaaaacg cgccgccagt acgtcagctg ccgcaccgtg cgcgacacag cccgcacagg
    14521 cggcatcata cggcgacagt ttttgcccaa gcaatgcgcc aataatacca gagagcacat
    14581 cgcccatgcc gccgctcgcc atgcctgcat ttccggcatc aataatgcct aaagcgtcag
    14641 gatgggcggc gaccacggtt ccggcacctt tcagcaccgc tacgccgcca taacgttgta
    14701 ccagacgttt ggcgcaatgt aagcggtcac tttcaatttc agcgacggaa cagcctaaca
    14761 accgtgcggc ctcgccagga tgcggcgtga tcacgcgatt gtgacgctta tcgggattga
    14821 ttgccagcag gttcaatgca tcggcatccc acaacatcgg tttgcgaaaa ttctcaactt
    14881 tttgcagtgc ttttttcccc cactcttgct ggcccagacc gggaccaatc accaccacat
    14941 cgccccattc caggctttcg gtaagagagt ccatcgtcag ttcatgcacc atcaattccg
    15001 gtcgtgcagt cagcagcggc gcaatgtttt cactgcgggt cagtactcgg actaaaccag
    15061 caccagcacg cagcgccgct tcccccgtca tacgaatagc ccccgccgtg ccgtgatcgc
    15121 caccgataat taccagccgc ccgtgatcgc ctttatgcga agtcgggcga cgcggtttta
    15181 gccagtgaga aagttgttct gctgaaaacc gctgaatttt cgtctcctga cctgccagcc
    15241 aactatccag ccccagtgag tcaaaatgca gttgtccggt aacatcccgc gcttttccag
    15301 tgagcaagcc tggtttcagc gcaataaaag tgatggagtg atcggcgttg atcaccgcgc
    15361 ctggcgtagc gccagtttca gccagcaggc cggaagggat atcaaccgcc acaatcggcg
    15421 caggatggga attagcgtgg tcgattaact ggctaatgga ttcgcggggc gcttgccgca
    15481 aaccggtacc gagcagcgca tcaacaatca gatctaccga ttcgggccag acaatattcg
    15541 aagcatggat ctcgccaccc gcgtttaacc atgcttcgcg tgccagcgcg gcctcttccg
    15601 gcaacggttt gtcgctctcc tgggcaaaca acgtgacctc aatgccgacc gctttggcca
    15661 gtcgcgcgac cacgtagcca tcgccgccgt tattaccatg accgcacagc accagccagt
    15721 ggcgggcgtc aggatacgcc gaacgacaca cctggaatgc ggcctcgcca gcgcgaagca
    15781 tcagctcata gagtgtgagc cccagcacat ctgccgcctc gcgttctccg cggcggatat
    15841 cgtcggcgta ccagacggtg tgtggtatac ttacggggtt tttcttcatt gtatggtccg
    15901 tcatgtcaga gcccctcgat ctcaatcagt tagcgcaaaa aattaaacag tgggggctgg
    15961 aactgggctt tcagcaggta ggtattaccg ataccgatct cagcgagtcc gagcccaaac
    16021 tgcaagcatg gctggacaaa caataccacg gcgaaatgga ctggatggca cgtcacggta
    16081 tgctgcgcgc tcgccctcat gagttattgc ccggtacgct gcgcgtgatc agcgtgcgga
    16141 tgaattacct tcctgctaac gccgcatttg ccagcacgct gaaaaacccc aaactcggct
    16201 atgttagccg ttatgcgctg ggccgtgact atcacaaact tctgcgcaac cgactcaaaa
    16261 agctgggcga gatgattcag caacattgtg tttcgctgaa ttttagaccg tttgtcgatt
    16321 ctgcgcctat tctcgagcgc ccgttagctg aaaaagctgg gctcggctgg acaggtaagc
    16381 actcacttat cctcaatcgc gaggccggtt cgttcttctt tttaggcgaa ttgctggtcg
    16441 atattccgct gcccgtggat caaccagtcg aggaaggatg cggtaaatgc gtggcctgta
    16501 tgacgatttg cccgaccggt gccatcgtcg agccatatac cgtcgatgct cgccgctgta
    16561 tctcttatct caccatcgaa cttgaagggg cgatcccgga agagttgcga ccgttaatgg
    16621 gaaaccgtat ttacggttgc gatgactgcc agcctatctg cccgtggaat cgctattcac
    16681 aactcaccac agaagaggat ttcagcccgc gtaagccgct acacgcaccg gaactcattg
    16741 agttattcgc ctggagcgaa gagaagtttt taaaagtcac ggaaggatcg gcgattcgtc
    16801 gaattggtca cctgcgttgg ctgcgtaata tcgccgtagc attaggcaat gccccttggg
    16861 atgaaacgat tttgacagcg ctggaaagtc gtaaaggtgg gcacccactt ctcgatgagc
    16921 acatagcgtg ggcgattgcg cagcaaatag agagacgaaa tgcgtgcata gtcgaagtgc
    16981 aattgccgaa aaaacagcgt ctggttcggg tgattgaaaa agggttaccg cgtgacgcct
    17041 gattcatcca cagcttgtga ataaaaataa aaacgcattg caattcaaga cgaataaaat
    17101 cgtcaagtga tcgactcgac aaaacggaat cttaattttt aacttatcta tcaataggtt
    17161 aaaaaaatac tattcaccgt gcgaagtttc tttgaaatta agattacagc ctgaggctgt
    17221 ggataactct gtttacaaga gtttgtcaga gacaacgaaa accagcaata acgcatcgct
    17281 gtggatattt tattgagaga agaatttgga gcgggaaacg agact
//
LOCUS       DAJTSL010000080        17166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTSL010000080 DAJTSL010000000
VERSION     DAJTSL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.080"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(34..2631)
                     /gene="topA"
                     /locus_tag="OO433_002390"
     CDS             complement(34..2631)
                     /gene="topA"
                     /locus_tag="OO433_002390"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6050839.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            3011..3262
                     /locus_tag="OO433_002391"
     CDS             3011..3262
                     /locus_tag="OO433_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6050840.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(3298..4347)
                     /gene="sohB"
                     /locus_tag="OO433_002392"
     CDS             complement(3298..4347)
                     /gene="sohB"
                     /locus_tag="OO433_002392"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6050841.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMGSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKY
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4567..5325
                     /locus_tag="OO433_002393"
     CDS             4567..5325
                     /locus_tag="OO433_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6050842.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            5322..5913
                     /gene="cobO"
                     /locus_tag="OO433_002394"
                     /pseudo
     CDS             5322..5913
                     /gene="cobO"
                     /locus_tag="OO433_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(5953..6825)
                     /gene="rluB"
                     /locus_tag="OO433_002395"
     CDS             complement(5953..6825)
                     /gene="rluB"
                     /locus_tag="OO433_002395"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6050843.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(7036..8931)
                     /locus_tag="OO433_002396"
     CDS             complement(7036..8931)
                     /locus_tag="OO433_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HCR6050844.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENPEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSLF
                     YRPDTFLQWGGLAILAIILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(8959..9579)
                     /locus_tag="OO433_002397"
     CDS             complement(8959..9579)
                     /locus_tag="OO433_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6050845.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9576..10457)
                     /gene="yciV"
                     /locus_tag="OO433_002398"
     CDS             complement(9576..10457)
                     /gene="yciV"
                     /locus_tag="OO433_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6050846.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLTTLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10595..10639
                     /gene="trpL"
                     /locus_tag="OO433_002399"
     CDS             10595..10639
                     /gene="trpL"
                     /locus_tag="OO433_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6050847.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    10616..10709
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            10731..12293
                     /gene="trpE"
                     /locus_tag="OO433_002400"
     CDS             10731..12293
                     /gene="trpE"
                     /locus_tag="OO433_002400"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6050848.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRTGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGIVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12293..13888
                     /gene="trpD"
                     /locus_tag="OO433_002401"
     CDS             12293..13888
                     /gene="trpD"
                     /locus_tag="OO433_002401"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6050849.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQRKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13889..15250
                     /gene="trpCF"
                     /locus_tag="OO433_002402"
     CDS             13889..15250
                     /gene="trpCF"
                     /locus_tag="OO433_002402"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6050850.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFNPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     GLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPTHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15262..16455
                     /gene="trpB"
                     /locus_tag="OO433_002403"
     CDS             15262..16455
                     /gene="trpB"
                     /locus_tag="OO433_002403"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6050851.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVCEFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRETPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16455..>17166
                     /gene="trpA"
                     /locus_tag="OO433_002404"
     CDS             16455..>17166
                     /gene="trpA"
                     /locus_tag="OO433_002404"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6050852.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLRDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAI"
BASE COUNT         4118 a         4596 c         4298 g         4154 t
ORIGIN      
        1 cctttgttta taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc
       61 atcaacataa aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg
      121 ctgtttggtt ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg
      181 cgcatcggcc agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc
      241 caccagtggc gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaaaacacc
      301 cgcagcgccg tcacgcagca cgaaataagc atctgatttt tcgcacggca gctcaggtaa
      361 tggcaccgga tcttctttcg gtggtgccac ttcgccgtta cgtaaaatct tacgtgtgtt
      421 tttacactct tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat
      481 ttcagagcca catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc
      541 gccctcttcg atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg
      601 tttcggatcg atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc
      661 gcgcagcgcg ttggtttcag cgtcttcgcc ttccagtacg ttcagcactt cgttttccgg
      721 caccaggtta atggtggttt tgcaacgctc tttcggcggc agcgcataac cagaacagcc
      781 aaggaatacc ccggtgctcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc
      841 aatgctggtc agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc
      901 tttatctaac tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc
      961 ttcgtgattt gccacctggt cgaggctgtt ttccatctgt gcggtgaagt cgtagttcat
     1021 taactcgcgg aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata
     1081 gaaacgacga ttttctactc gcacgtagcc acgatcctga atggtcgaaa tgatcgacgc
     1141 ataggtagac ggacgaccga taccgcgttt ttccagctct ttaaccagcg atgcttcact
     1201 gaaacgggct ggcggcttgg taaagtgctg ggctggtgta agttcaacga gcgtcagagc
     1261 atcgccttta ttaactgctg gtaagatgcg atcttcatcg cctttacgca acgcaggcat
     1321 cacttttgtc cagccatcaa aacgcaaaat acgacctcgt gctttcaggc ggaaatcgcc
     1381 cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggctacgaa
     1441 ctgacgccag attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc
     1501 cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt
     1561 gctggcgtac tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata
     1621 accgcgaacc atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt
     1681 gatatagcct gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaatcc
     1741 aagacgggtg ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact
     1801 ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac
     1861 cgcagcctga gtttgttctt tgttgaccgg acggaacggt ttgtcgttct gatgagtcac
     1921 ctgtaacgcc aacgcttcac cagatggcgt ggtcgtgctg gcatcgactt cccagaactc
     1981 ttccggcacg aacgctttaa tttcacgctc acgctcgacc accaggcgaa ccgccaccga
     2041 ctgcacacga ccggcagaca gaccacgagc gatctttttc catagcagcg gcgaaaccat
     2101 ataccccacc acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt
     2161 cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac
     2221 cactcggcta tagcgcgcat catcaccccc aatcacttcc cgcaggtgcc atgcaatggc
     2281 ttccccttcg cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg
     2341 tttcagttca gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt
     2401 gtgccacggg tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt
     2461 tttagccgtc ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg
     2521 caaatcgcgg atgtgaccga cgctggattt caccacgtag tcactaccca gatacttgtt
     2581 gatcgttttg gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt
     2641 acctaatttg attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag
     2701 ttaccgatat ggactcgttc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa
     2761 aattcaggtg actgagttaa caggtcttgg cctacaggcg ctatagcacg acggaatttt
     2821 ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa
     2881 aatccgatac gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc
     2941 caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttatttaa
     3001 ggagtaaata atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc
     3061 caacaaaatc attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac
     3121 gcagcgtaat ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc
     3181 aacagccaaa agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga
     3241 aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta
     3301 catcaatggc ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc
     3361 cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac
     3421 ttcacggcct tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag
     3481 gcctttctct accgcctgtt gtccgtacca gtgttcacct gttgccacct gttcaatatc
     3541 cagagacgga cgcatacgct tcacaaaatc tttaaacaac tgatgcgttt cgttcaactc
     3601 ttcgcggaat ttctcccgcc cttcttcggt attttcaccc agcaacgtca gagtacgctt
     3661 atactgcccg gcagtgtgca gttcgatatc aatatatttg cttttcagga agcggttaaa
     3721 gttgggcatt tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat
     3781 tttgtccgcc acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac
     3841 agttaaagga atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc
     3901 atgcaccatg ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc
     3961 tgcgagtacc gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc
     4021 tttaaaatcc agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc
     4081 tttcgctttt gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg
     4141 tttttgctga tgtgagccca tcagcgccgc ggccagttct tctttcatct ccttatactg
     4201 ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt
     4261 gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tcgccaaaaa
     4321 caaaccatat tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct
     4381 cacagtttac aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg
     4441 acaaagtatc cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca
     4501 ggcataaagc cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga
     4561 gtagccatgc attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg
     4621 ggagccagcg atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca
     4681 gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa
     4741 gaaactgggc gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat
     4801 tgccaacaac tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat
     4861 aatgccggat tgctcggcga tatttgccca atgagcgaac aaaatccgca ggtctggcag
     4921 gacgtcatgc aggtcaacgt taatgccacc tttatgctca cccaggcact gcttccttta
     4981 ttactcaaat cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga
     5041 cgagctaact ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta
     5101 ctggccgatg aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc
     5161 accgcaatgc gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct
     5221 gatatcatgc cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg
     5281 acctttgacg cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc
     5341 aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg tgtggcacag gccccaggat
     5401 gaacgcggta ttatcatcgt ctttaccggc aatggaaaag gcaaaaccac cgcggcattt
     5461 ggtacggcaa cacgcgcagt tggtcacgga aaaaaagtgg gcgtcgtgca gtttattaaa
     5521 ggcacctggc ctaatggcga acgcaatctg ctggaaccac atggcgttga gtttcaggtg
     5581 atggcaacgg gctttacctg ggatacacaa aaccgcgagt ctgataccgc cgcctgccgc
     5641 gaagtctggc aacatgcaaa gcggatgctt gctgattcct cactggatat ggttttgctt
     5701 gatgaactga cgtatatggt ggcgtatgac tatttgccac tggaagaagt ggtgcaggcg
     5761 ttaaatgaac gtccacatca acagacggtg attatcacgg gtcgaggctg tcatcgggat
     5821 attcttgaac tggcggatac ggtaagtgag ctgcgcccca tcaaacatgc gtttgatgcc
     5881 ggtgttaagg cgcaaatagg gattgattat taatctgtaa aaatgccgga tatgacgtcc
     5941 ggcatacttt gattaaccgt tattacgccc gccagaacga cgaccgccgc tcacctgact
     6001 gtgacgtttc accgcacgac gaatctgatt agccttcatg cgacgacggt ctttttctac
     6061 agcgacttta gagctggttt ctggcggaag ctccaccagt tcgcgcagat agttagtctg
     6121 ggcgaggtcc agctccgtcc agccaccgcg tggcagacct tttggcagtg ggatatcacc
     6181 gtaacgaaca cggatcaggc ggctcacctg cacaccaacc gcttcccaca gacgacgaac
     6241 ttcacggtta cggccttcgg tcagagtcac gttgtaccac tggttgatcc cttcgccgcc
     6301 gctgaacttg atggttttaa aagctgccgg accatcttcc agttgcacgc cacgactcaa
     6361 atcacgcagt ttcgcgtcgt caacctgacc aaatacacgc acggcgtatt cacgttcaac
     6421 ttcacggctt ggatgcatta aacggtttgc cagttcacca tcggtggtga acagcagcag
     6481 accacaggtg ttaacgtcca gacgacctac ggcaatccag cgtgcgccac gcagctttgg
     6541 cagacggtca aacaccgtcg gacgcccttc cggatcgtta cgggtacaca actcaccttc
     6601 cggtttgtaa taggccagaa cgcgacaaat ttgttcagcg gactcacgta ccgaaatcag
     6661 atgaccatcg atacgaattt tcagcccagg ggtaacttca acgcgatcgc cgagtttagc
     6721 aattttgcca tcaacactca cacgaccggc ttcaataata gattcgattt cacggcggga
     6781 gccgtggcca gcgcgggcca gcactttctg taatttttcg ctcatagagc ttccttttgg
     6841 tgtcgccttc ccaggcgtct gatatgcttt tgtgcaacag tgagtcagaa cttacatctg
     6901 actcactgat agtaaagtct gtttgaactc ttcaggcctt aagtaaacga ctcgacgttt
     6961 atcggcctgg ctttgaggcg caacatacta cactatcttt atggttaaag cttgtttcga
     7021 ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcgccgc cgccagcacc
     7081 accaccggaa taacttcgcc ccaacaaact ggtattggcc ccgcaactta ggtgcgtctg
     7141 taatgagaat aaaacgccgt tacgggcaat ctctggagcg cgaatcgccg aagaaagcga
     7201 aggggcaatg cgtaacgccc attccctccc caaatttgcc gatatagccc aacctagcaa
     7261 cgcgtaatcc agtttccgtg tttccccttt ccgccgacgt ccactgtgtg ttgctgcctc
     7321 tttacgcgca agatagttaa cgatagcttc accgcgggca taacgttgct gcccagtttg
     7381 ggtaaacttt ggcagaaaaa tataccccat gccagtgaga taaccggtca ggcaaacagc
     7441 gctaaaataa cccgctggca tataccagta aaagaataca tctccgacat tgatatagag
     7501 agaatagaag gccacgcctg aacaaatcag tggtaaaaac aaagtgatga ttgcacgatt
     7561 aacgcaaggc ttgtcatcat cgcgggcaaa caaaagctca agggaacaaa ataaaaaagg
     7621 aataatccag cagatcataa taaacatatc gcctaccaga ctcattcctg cccaacctac
     7681 gggactcagg ttaccgtaga gaatgatcgc caatattgcc agtccacccc attgcaaaaa
     7741 cgtatcaggc cggtaaaaca aagacttttt acgcggcaga taaaatttct gcatccgtaa
     7801 aaatgccctg cgtaagactc tgttgcgtct ccccgtaaga acgaccttat cgcctttacg
     7861 aaataacgct tccagtaata actgctctgc gggcagtaat acctcattat ttctggtcgc
     7921 gccttcatta acacaaatca ggatttgctg gttcttatca tactgatctt ccagatgaat
     7981 ttttcccttc acgatcagat ttacgatatc ggcacagaaa cctttatcgt cgtaaaccag
     8041 cttcgcatcg agacgtaaca tgccgggcgt gtaatctggc ggaatgacat cggtttcaat
     8101 cacatctacc ggcgtaaatt gcggcctgcg cttccagaga tataaccatc cacaaaccag
     8161 gaatagacac ggaaaccaaa ttaacaacga tgacgtggag ggtaaaagaa gatggctgaa
     8221 aatattcgtc gtttgtggag ccggggcatt gctaagtaac gcgtgaggcc agcggtagag
     8281 taccgtgaaa tcttcacgat aaaacggatc gcgggattct attcttccgt cctcaaggat
     8341 gcggccattt cgatatgtgt cgccctcttc tccagtaaag agatcgattt cgctaaatgg
     8401 attacccgcg atatcaggga acttgagtga aaatcgggtc ttatagattt caaacggcca
     8461 gtggttacca gtcacgttcc agattaacag atctgaatct ccctcacgca ggaaagcatt
     8521 tttaacctga taatgaattt gataatgata gtcgccttga ggcaagaaac gttgtttatc
     8581 gcccacgaca atactcatta acccgatatg ccagtcaaga tgccagggtt caggaatacc
     8641 atcacgagtg acgccaacaa tatgatagtt cagcagtgca gcgtccccat caggctgcat
     8701 ccagagtcgt ggtaaatcac gataaaatcc gtgcttaatc tgctgatttc gcgcctgaaa
     8761 atgaatattt tctttgatgt cgagcacccc atccggttgc agagtggctt caacatcaaa
     8821 gagcgagatc ccttcttcat aagaagggat ctccgggttt tctgcataac ttagagatga
     8881 tacaaacagc cctgctatca acagcaaaat gcagcgaaac ttccctgcca tagtcttcca
     8941 tccgtggaac gcaatttgtc ataagaaagg cttcacatca cccacgcctt cacgcaccac
     9001 gaccggcgta tcatcggtaa gatcaatgac cgttgtcggt ttctgtccga gatagccgcc
     9061 gtggataatc aaatccacct gtttttccag acggtcttta atttcttctg gatcggattc
     9121 ggtaaattcg ctgcctggca gcattagcga agtggaaagc atcggttcgc ccagtgcttc
     9181 aagcaacgcc tgcgcaatcg ggttagaagg cacacgcata ccgatggttt tgcgtttttc
     9241 ctgcaacaga cggcgtggca cctccttcgt ccccttcagg atgaaggtat agttgcccgg
     9301 cgtgttgttt ttcattaaac gaaacgcaac gttatccacg aatgaatagg tcgaaagttc
     9361 agaaagatcg cgacacatca gggtaaagtt gtgaccgtcc ggcagctggc gaatacgaca
     9421 aatacgctcc atcgcgtttt tatcttcaat tttacagccg agcgcatagc cggaatcagt
     9481 tggataaaca atcaccccgc ctttacgcac gatctccacc gcctggttga tcagacgttg
     9541 ctgcgggtta tcaggatgaa tataaaaaaa ctggctcata attccctctc tgtggtgttc
     9601 tgcggctgtt cccatagctg ccagacgcct tcaacgcctg cgggcagcca gagtttacga
     9661 cccagttcga tccacgggca tggctgatga aaatcagatc cttgtgatgc ccataaatga
     9721 tgctgacgcg caagtgtcgt caactgggtg cgttcattgg gcgattgctg gcactgcgcg
     9781 acttccatcg cgtcgccgtg gtgttcggca aaatgcgcaa ccaatctttt cagccattta
     9841 gcactaagat tgtaccgccc aggatgagct agcaccgcct taccgccaga atgatgaatg
     9901 acatcaatag cttgttctat tgtacaccac tgtggcggaa cgtatccggt tttcccgcgc
     9961 gccagatact ttttaaagac atctgccatt gaactggctt tgccgcactc aacaaggaaa
    10021 cgcgcaaaat gaccgcgcgt cactgcgccg ccctgcgcca gtcgttgtgc gccttccagc
    10081 gccccaggta tttgcgcttt ttcaagtctt tcggcaattt gctgcgcccg ctgattgcgc
    10141 cgttctgtct gctgcgcgag gaactcacac attagcggat gagtaatatc aatattcagc
    10201 ccgacaatat gaatttcatg attttcccag accgtggaaa tttccacgcc gggaataaga
    10261 ttcagcgcca gtcccgaacg tgaaatttcc tctcttgctg gcgcgattgc agctgtggtg
    10321 tcatggtcgg tgatcgccag ggtgccgacg cgcatctcga ctgcacggtg caccaatgct
    10381 tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgcg
    10441 taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat
    10501 aacggttctg gcaaatattc tgaaatcagc tgttgacaat taatcatcga actagctaac
    10561 tagtacgcaa gttcacgtaa aaagggtatc gacaatgaaa gcaattttcg tactgaaagc
    10621 ttggtggcgc acttcctgaa acgggcagtg tattcaccat gcgtaaagca atcagatacc
    10681 cagcccgcct aatgagcggg cttttttttg aacaaaatta gagaataaca atgcaaacac
    10741 aaaaaccgac tctcgaactg ctaacctgcg aaggcgctta tcgcgacaac ccgactgcgc
    10801 tttttcacca gttgtgtggg gatcgtccgg caacgctgct gctggaatcc gcagatatcg
    10861 acagcaaaga tgatttaaaa agcctgctac tggtagacag tgcgctgcgc attacagcat
    10921 taggtgacac tgtcacaatt caggcacttt ccggcaacgg cgaagccctg ctgacactac
    10981 tggataacgc cctgcctgcg ggtgtggaaa atgaacaatc accaaactgc cgtgtgctgc
    11041 gcttcccccc tgtcagtcca ctgctggatg aagacgctcg cttatgctcc ctttcggttt
    11101 ttgacgcttt ccgtttattg cagaatctgt tgaatgtacc gaaggaagaa cgagaagcaa
    11161 tgttcttcgg cggcctgttc tcttatgacc ttgtggcggg atttgaagat ttaccgcaac
    11221 tgtcagcgga aaataactgc cctgatttct gtttttatct cgctgaaacg ctgatggtga
    11281 ttgaccatca gaaaaaaaac acccgcattc aggccagcct gtttgctccg aatgaagaag
    11341 aaaaacaacg tctcactgct cgcctgaacg atcttcgcca gcagctgacc gaagccgcgc
    11401 cgccgctgcc ggtggtttcc gtgccgcata tgcgttgtga atgtaaccag agcgatgaag
    11461 agttcggtgg cgtggtgcgt ttgttgcaaa aagcgattcg caccggagaa attttccagg
    11521 tggtgccgtc tcgccgtttc tctctgccct gcccgtcacc gctggcggcc tattacgtgc
    11581 tgaaaaagag taatcccagc ccgtacatgt tttttatgca ggataatgat ttcaccctgt
    11641 ttggcgcgtc gccggaaagt tcgctcaagt atgacgccac cagccgccag attgagatct
    11701 acccgattgc cggaacacgc ccgcgcggtc gtcgcgccga tggttcactg gacagagacc
    11761 tcgacagccg catcgaactg gaaatgcgta ccgatcataa agagctttct gaacatctga
    11821 tgctggtgga tctcgcccgt aatgatctgg cacgcatttg cacccccggc agccgctacg
    11881 tcgccgatct taccaaagtt gaccgttact cttacgtgat gcacctggtc tcccgcgtgg
    11941 tcggtgagct gcgccacgat ctcgacgccc tgcacgctta ccgcgcctgt atgaatatgg
    12001 ggacgttaag cggtgcgccg aaagtacgcg ccatgcagtt aattgccgag gcggaaggtc
    12061 gtcgccgcgg cagctacggc ggcgcggtag gttatttcac cgcgcatggc gatctcgaca
    12121 cctgcattgt gatccgctcg gcgctggtgg aaaacggtat cgccaccgtg caagccggtg
    12181 ctggcatagt ccttgattct gttccgcagt cggaagccga cgaaacccgt aataaagccc
    12241 gcgctgtact gcgcgctatt gccaccgcgc atcatgcaca ggagactttc tgatggctga
    12301 cattctgctg ctcgataata tcgactcttt tacgtacaac ctggcagatc agttgcgcag
    12361 caatgggcat aatgtggtga tttaccgcaa ccatattccg gcgcagacct taattgaacg
    12421 cctggcgacg atgagcaatc cggtgctgat gctttctcct ggccccggtg tgccgagcga
    12481 agccggttgt atgccggaac tcctcacccg cttgcgtggc aagctgccca ttattggcat
    12541 ttgcctcgga catcaggcga ttgtcgaagc ttacgggggc tatgtcggtc aggcgggcga
    12601 aattctccac ggtaaagcct ccagcattga acacgacggt caggcgatgt ttgccggatt
    12661 aacaaacccg ctgccagtgg cgcgttatca ctcgctggtt ggcagtaaca ttccggccgg
    12721 tttaaccatc aacgcccatt ttaatggcat ggtgatggca gtacgtcacg atgcggatcg
    12781 cgtttgtgga ttccagttcc atccggaatc cattcttact acccagggcg ctcgcctgct
    12841 ggaacaaacg ctggcctggg cgcagcggaa actagagcca accaacacgc tgcaaccgat
    12901 tctggaaaaa ctgtatcagg cgcagacgct tagccagcaa gaaagccacc agctgttttc
    12961 agcagtggta cgtggcgaac tgaaaccgga acaactggcg gcggcgctgg tgagcatgaa
    13021 aattcgcggc gagcacccga acgagatcgc cggagcagca accgcgttac tggaaaacgc
    13081 cgcgccgttc ccgcgcccgg attatctgtt tgccgatatc gtcggcaccg gtggtgacgg
    13141 cagtaacagc atcaatattt ccaccgccag tgcgtttgtc gccgcggcct gtgggctgaa
    13201 agtggcgaaa cacggcaacc gtagcgtctc cagtaaatct ggttcgtccg atctgctggc
    13261 ggcgttcggt attaatcttg atatgaatgc cgataaatcg cgccaagcgc tggatgagtt
    13321 aggcgtatgt ttcctctttg cgccgaaata tcacaccgga tttcgccatg cgatgccggt
    13381 tcgccagcaa ctgaaaaccc gcactctgtt caacgtgctg ggaccattga ttaacccggc
    13441 gcatccgccg ctggcgttaa ttggtgttta tagtccggaa ctggtgctgc cgattgccga
    13501 aaccttgcgc gtgctggggt atcaacgcgc ggcggtagtg cacagcggcg gtatggatga
    13561 agtttcatta cacgcgccga caatcgttgc cgaactgcat gacggtgaaa ttaagagcta
    13621 tcagctaact gcagaagatt ttggcctgac accctaccac caggagcaac tggcaggcgg
    13681 aacaccggaa gaaaaccgtg acattttaac acgcttgtta caaggtaaag gcgacgccgc
    13741 tcacgaagca gccgtcgcgg cgaatgtcgc catgttaatg cgcctgcatg gccatgaaga
    13801 tctgcaagcc aatgcgcaaa ccgttcttga ggtactgcgc agtggttccg cttacgacag
    13861 agttaccgca ctggcggcac gagggtaaat gatgcaaacc gttttagcga aaatcgtcgc
    13921 agacaaggcg atttgggtag aaacccgcaa acagcagcaa ccgctggcca gttttcagaa
    13981 tgaggttcag ccgagcacgc gacattttta tgatgcgcta cagggtgcac gcacggcgtt
    14041 tattctggag tgcaagaaag cgtcgccgtc aaaaggcgtg atccgtgatg atttcaatcc
    14101 ggcacgcatt gccgccattt ataaacatta cgcttcggca atttcggtgc tgactgatga
    14161 gaaatatttt caggggagct ttgatttcct ccccatcgtc agccaaatcg ccccgcagcc
    14221 gattttatgt aaagacttca ttatcgaccc ttaccagatc tatctggcgc gctattacca
    14281 ggccgatgcc tgcttattaa tgctttcagt actggatgac gaccaatatc gccagcttgc
    14341 agccgtcgct cacagtctgg agatgggggg gctgaccgaa gtcagtaatg aagaggaact
    14401 ggagcgcgcc attgcattag gggcaaaggt cgttggcatc aacaaccgcg atctgcgtga
    14461 tttgtcgatt gatctcaacc gtacccgcga gcttgcgccg aaactggggc acaacgtaac
    14521 ggtaatcagc gaatccggca tcaatactta cgctcaggtg cgcgagttaa gccacttcgc
    14581 taacggtttt ctgattggtt cggcgttgat ggcccatgac gatttgcacg ccgccgtgcg
    14641 ccgggtgttg ctgggtgaga ataaagtatg tggcctgacg cgtgggcaag atgctaaagc
    14701 agcttatgac gcgggcgcga tttacggtgg gttgattttt gtcgcgacat caccgcgttg
    14761 cgtcaacgtt gaacaggcgc aggaagtgat ggctgcggca ccgttgcagt atgttggcgt
    14821 gttccgcaat cacgatattg ccgatgtggt ggacaaagct aaggtgttat cgctggcggc
    14881 agtgcaactg catggtaatg aagatcagct gtatatcgat acgctgcgtg aagctctgcc
    14941 aacacatgtt gccatctgga aagcattaag cgtcggtgaa accctgcccg cccgcgagtt
    15001 tcagcacgtt gataaatatg ttttagacaa cggccagggt ggaagcgggc aacgttttga
    15061 ctggtcacta ttaaatggtc aatcgcttgg caacgttctg ctggcggggg gcttaggcgc
    15121 agataactgc gtggaagcgg cacaaaccgg ctgcgccgga cttgatttta attctgctgt
    15181 agagtcgcaa ccgggcatca aagacgcacg tcttttggcc tcggttttcc agacgctgcg
    15241 cgcatattaa ggaaaggaac aatgacaaca ttacttaacc cctattttgg tgagtttggc
    15301 ggcatgtacg tgccacaaat cctgatgcct gctctgcgcc agctggaaga agcttttgtc
    15361 agcgcgcaaa aagatcctga atttcaggct cagttcaacg acctgctgaa aaactatgcc
    15421 gggcgtccaa ccgcgctgac caaatgccag aacattacgg ctgggacgaa caccacgctg
    15481 tatctgaagc gcgaagattt gctgcacggc ggcgcgcata aaactaacca ggtgctcggt
    15541 caggctttac tggcgaagcg gatgggtaaa accgaaatca tcgccgaaac cggtgccggt
    15601 cagcatggcg tggcgtcggc ccttgccagc gccctgctcg gcctgaaatg ccgtatttat
    15661 atgggtgcca aagacgttga acgccagtcg cctaacgttt ttcgtatgcg cttaatgggt
    15721 gcggaagtga tcccggtaca tagcggttcc gcgacgctga aagatgcctg taacgaggcg
    15781 ctgcgcgact ggtccggcag ttatgaaacc gcgcactata tgctgggcac cgcagctggc
    15841 ccacatcctt atccgaccat tgtgtgtgag tttcagcgga tgattggcga agaaacgaaa
    15901 gcgcagattc tggaaagaga aggtcgcctg ccggatgccg ttatcgcctg tgttggcggc
    15961 ggttcgaatg ccatcggcat gtttgcagat ttcatcaacg aaaccaacgt cggcctgatt
    16021 ggtgtggagc cgggtggtca cggtatcgaa actggcgagc acggcgcacc gctaaaacat
    16081 ggtcgcgtgg gcatttattt cggtatgaaa gcgccgatga tgcaaaccga agacgggcag
    16141 attgaagaat cttactccat ctccgccgga ctggatttcc cgtctgtcgg cccacaacac
    16201 gcgtatctta acagcactgg acgcgctgat tacgtgtcta ttaccgatga tgaagccctt
    16261 gaagccttca aaacgctgtg cctgcacgaa gggattatcc cggcgctgga gtcttcccac
    16321 gccctggccc atgcgctgaa aatgatgcgc gaaaccccgg aaaaagagca gctactggtg
    16381 gttaaccttt ccggtcgcgg cgataaagac atcttcaccg ttcacgatat tttgaaagca
    16441 cgaggggaaa tctgatggaa cgctacgaat ctctgtttgc ccagttgaag gagcgcaaag
    16501 aaggcgcatt cgttcctttc gtcacgctca gggatccggg cattgagcag tcattgaaaa
    16561 ttatcgatac gctaattgaa gccggtgctg acgcgctgga gttaggtatc cccttctccg
    16621 acccactggc ggatggcccg accattcaaa acgccactct gcgcgccttt gcggcaggtg
    16681 tgactccggc acaatgtttt gaaatgctgg cactgattcg ccagaaacac ccgaccattc
    16741 ccattggcct gttgatgtat gccaatctgg tgtttaacaa aggcattgat gagttttatg
    16801 cccagtgcga gaaagtcggc gtcgattcgg tgctggttgc cgatgtgcca gttgaagagt
    16861 ccgcgccctt ccgccaggcc gcgttgcgcc ataatgtcgc acctatcttc atctgcccgc
    16921 caaatgccga tgacgacctg ctgcgccaga tagcctctta cggtcgtggt tacacctatt
    16981 tgctgtcacg agcgggcgtg accggcgcag aaaaccgcgc cgcgttaccc ctcaatcatc
    17041 tggttgcgaa gctgaaagag tacaacgctg cacctccatt gcaggggttt ggtatttccg
    17101 ccccggatca ggtaaaagca gcgattgatg caggagctgc gggcgcgatt tctggttcgg
    17161 ccattg
//
LOCUS       DAJTSL010000081        17105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTSL010000081 DAJTSL010000000
VERSION     DAJTSL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.081"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..304)
                     /gene="hlyE"
                     /locus_tag="OO433_002405"
     CDS             complement(<1..304)
                     /gene="hlyE"
                     /locus_tag="OO433_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCR6050853.1"
                     /translation="MTEIVADKMVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLF"
     gene            677..1096
                     /locus_tag="OO433_002406"
     CDS             677..1096
                     /locus_tag="OO433_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6050854.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1096..2363
                     /gene="umuC"
                     /locus_tag="OO433_002407"
                     /pseudo
     CDS             1096..2363
                     /gene="umuC"
                     /locus_tag="OO433_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(2409..2939)
                     /gene="dsbB"
                     /locus_tag="OO433_002408"
     CDS             complement(2409..2939)
                     /gene="dsbB"
                     /locus_tag="OO433_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6050855.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(3085..4626)
                     /gene="nhaB"
                     /locus_tag="OO433_002409"
     CDS             complement(3085..4626)
                     /gene="nhaB"
                     /locus_tag="OO433_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6050856.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4848..5567
                     /gene="fadR"
                     /locus_tag="OO433_002410"
     CDS             4848..5567
                     /gene="fadR"
                     /locus_tag="OO433_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6050857.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5619..7151)
                     /locus_tag="OO433_002411"
     CDS             complement(5619..7151)
                     /locus_tag="OO433_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6050858.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7481..8779
                     /gene="dadA"
                     /locus_tag="OO433_002412"
     CDS             7481..8779
                     /gene="dadA"
                     /locus_tag="OO433_002412"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6050859.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8789..9848
                     /gene="dadX"
                     /locus_tag="OO433_002413"
                     /pseudo
     CDS             8789..9848
                     /gene="dadX"
                     /locus_tag="OO433_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(10235..11971)
                     /locus_tag="OO433_002414"
     CDS             complement(10235..11971)
                     /locus_tag="OO433_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6050860.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGTAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(12066..12980)
                     /gene="ldcA"
                     /locus_tag="OO433_002415"
     CDS             complement(12066..12980)
                     /gene="ldcA"
                     /locus_tag="OO433_002415"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6050861.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVSAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            13080..13691
                     /gene="emtA"
                     /locus_tag="OO433_002416"
     CDS             13080..13691
                     /gene="emtA"
                     /locus_tag="OO433_002416"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6050862.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13693..14427)
                     /locus_tag="OO433_002417"
     CDS             complement(13693..14427)
                     /locus_tag="OO433_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6050863.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14628..14882
                     /locus_tag="OO433_002418"
     CDS             14628..14882
                     /locus_tag="OO433_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6050864.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFQTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGLFRLLRRE"
     gene            15061..15501
                     /locus_tag="OO433_002419"
     CDS             15061..15501
                     /locus_tag="OO433_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050865.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPAV"
     gene            complement(15580..17105)
                     /locus_tag="OO433_002420"
                     /pseudo
     CDS             complement(15580..17105)
                     /locus_tag="OO433_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
BASE COUNT         4311 a         4225 c         4422 g         4147 t
ORIGIN      
        1 caaatagcaa aatatacgct gcgagcaatt gcgtcgcaac accacaccat tcatacactg
       61 tttgggttgc ttcaaaatac ttatcctggc tatccataag taaggtttta atatcgccga
      121 ctaaaacgga ggctgcctgt gaatactcct gtttaaagcg acttaactct tttatggttt
      181 catcaaaggt ctgccagggg atgacctgat cgagatattt attataaaga tctaatgctc
      241 catctgcggt ttcgattgcg tttttaacta cttctaccat tttatctgca acgatttcag
      301 tcataatcat tcgcctcttt aaatatataa attgtaatga aactcctgtt ttacaactat
      361 taataaattt tacttcatct aattcatagt tagccgggtg ggatgcgtca atgtctttat
      421 ttctattaat atgataaata tcaaacaatg tttaatgtca ttatggcgaa tgcttctatt
      481 ctattttttt gccgggtgat atttttcatt tctgctggat gagcgtcgtc gccagaaggc
      541 cacgtgagca caggataaga gaacgaaaaa tcagcagtct atacagcgac aaatattgat
      601 agcctgaatc agtattgatc tgctggcaag aatagactac tgtatataaa aacagtataa
      661 cttcaggcag attattatgt tgtttatcaa gcctgcggat ctccgcgaaa ttgtgacttt
      721 tccgctattt agcgatcttg ttcagtgtgg ctttccttca ccggcagcag attacgtcga
      781 acagcgcatc gatctgaatc aactgttgat ccagcatccc agcgcgactt acttcgtcaa
      841 agcaagtggt gattctatga ttgatggtgg aattagtgac ggtgatttac tgattgtcga
      901 tagcgctatt accgccagcc atggtgatat tgtcatcgct gctgttgacg gcgagtttac
      961 ggtgaaaaaa ttgcaactac gcccgacggt acagcttatt cccatgaaca gtgcgtactc
     1021 gcccattacc atcagtagcg aagatacgct ggatgtcttt ggtgtggtga tccacgtcgt
     1081 taaggcgatg cgctgatgtt tgctctctgt gatgtaaacg cgttttatgc cagctgtgag
     1141 acggtgtttc gccctgattt atggggtaaa ccggtggttg tactatcgaa taatgacggt
     1201 tgcgttatcg cccgaaacgc tgaggcaaag gcgctgggtg ttaaaatggg cgatccctgg
     1261 ttcaaacaaa aagatctgtt tcgtcgctgt ggtgtggttt gctttagcag caattatgag
     1321 ctttacgcag acatgagcaa tcgggtgatg tcgacgctgg aagaactatc gccccgcgtc
     1381 gagatttaca gtattgatga ggcattctgc gatctgacag gtgtgcgtaa ttgtcgcgat
     1441 ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca tcttactgtt
     1501 ggtgtgggga tcgcccagac caaaacgctg gctaagcttg ccaatcatgc ggcaaaaaaa
     1561 tggcagcggc agacgggggg gtggtggatt tatcaaatct ggaacgccag cgtaaattaa
     1621 tgtctgctct ccccgtggat gacgtctggg ggattggacg gcggatcagc aaaaaactgg
     1681 acgcgatggg gatcaaaacc gttcttgatt tggcggatac agatatccgg tttatccgta
     1741 aacattttaa tgtcgtgctc gaaagaacgg tgcgtgaact gcgcggtgaa ccctgtttgc
     1801 aactggaaga atttgcaccg acaaagcagg aaattatctg ttcccgctcg tttggtgaac
     1861 gtatcacgga ttatacgtcg atgcggcagg ccatttgtag ttacgctgcc cgggcggcgg
     1921 aaaaacttcg cagtgagcat caatattgtc ggtttatctc cacatttatt aagacgtcac
     1981 catttgcgct caatgaacct tattacggca atagcgcgtc ggtaaaactg ctgacgccca
     2041 ctcaggacag cagggatatc attaacgccg ctacgcgatc tctggatgcc atctggcaag
     2101 cgggccatcg ctaccaaaaa gcgggcgtga tgctggggga tttcttcagt cagggagtcg
     2161 cgcagctcaa tttattcgat gacaacgcac cgcgccccgg gagtgagcaa ttgatgacgg
     2221 taatggatac actgaatgct aaagagggca gaggaacact ctattttgcc gggcagggga
     2281 tccaacaaca atggcagatg aagcgagcca tgctttcacc acgttataca acgcgaagtt
     2341 ctgatttact gcgggtcaaa taaatatagc ggcaggaaaa aagtgctccc gcaggagcgc
     2401 cgaatggatt agcgaccgaa cagatcacgt tttttcgctt taaacggctg ggaaatcatc
     2461 accagcaccg cgacaatcag gtaagcgata aaaataccga gcagccactg cggcatttcc
     2521 agacctaaaa aatcccactg acgctcggcg caatcgccag aggcgacaaa cacttgcggc
     2581 acccatttat ccagcggcag ccattccggg aaacgaacca taaaatcaca ggtggcaaac
     2641 ggcgaaggat agagctgaag catggtgtgc tcgtaagtta actgcacacc gcggaacgca
     2701 ctatacaacc agataaccat cgctacataa cgcagcggag ttttcggggc gatcgcgcca
     2761 atcagcgcag cacccagaac gccgaataac gcgcagcgtt cataaataca gagcacgcaa
     2821 ggtttcagta acatcacatg ctggaaccac agcgccgtca gttccagtgc cagagcagta
     2881 aacgccatca acagccacgc gccccggcct tgtgaacatt ggttcaaaaa tcgcaacata
     2941 atcatttccc tgcaatatgc atagagtgcg cagtttaaac caattcattc gctgcgccac
     3001 caggaggcag caaaaatagg acgcaattgg acaattatcg ggcgaaaagg caaaccgggc
     3061 atttatatgc ccggtaagtt gttatcaaag cgttgctatc cagcccattt gcataaacca
     3121 ttcggttaca ggggcaagcg taaactcgac gcagagcagc ccgacgagtg tcaggacgag
     3181 ggtgtaaggc agggccatcc acaccatgcg gccataagag aggcgaatca atggcgcgag
     3241 tgcagaggtc agcaggaaca ggaacgcagc ctgaccgttc ggcgtagcga cagagggcag
     3301 attggtaccg gtattaatgg cgaccgccag cagctcgtat tgcttcaacg tgatagcgcc
     3361 actttccatt gccgctttcg cttcgttgat ataaatcgtc cccacgaaga cgttatccga
     3421 aatggatgac agcagaccgt tgaaaatata gaacaacgac agctgagcat gttccgatgc
     3481 ctgcaacaca aactgaataa tcggcgaaaa cagttgttgg tcgataatca ccgcgactac
     3541 cgagaaaaac accgtcaaca gtgcggtgaa tggcagagat tcggtgaagg ctttaccgat
     3601 agcatgttca tcggtgacac cggtcaatga tgtcgccaga ataatgaccg acaaaccaat
     3661 caagccaacc tccgccagat gcaacgccag cgcagtcacc agccagacgc cgataatcgc
     3721 ctggacaatc agacggattt tatcctgacg ggtacgctgg tggcggcttt gatcgtcaaa
     3781 ctgttgcaac acttcgcgga ctttctccgg cagcgtttca ccgtagccaa accaacgcag
     3841 cttctctacc agcaggcagg ttaacaggcc acaaatcaga accggaacgg tcaccggcga
     3901 catgcgcagg aagaaatcgc caaaatgcca gccagccgct ttagcgatga tcaggttctg
     3961 tggttcgccc accatggtca ttacgccgcc taatgcggtg ccgacaccgg catgcatcat
     4021 caggctacgc agaaagccac ggaactgttc cagaaccact ttgtaatgct tgtcgatatg
     4081 actatcgtct tgcaggtcgg tatcttcagt acgggaagag gctacgcggt gataaatacc
     4141 ataaaaaccg actgcaacgc tgatcaccac cgccacgacg gttaaggcat cgaggaacgc
     4201 ggagaggaac gccgccgcca cgcaaaaaga gagcgacagc agcattttgg aacgaatgct
     4261 taacagcaaa cgggtaaata tgaacagcaa tagctgtttc ataaaataga tacccgccac
     4321 cataaacatc agtaacagca agacttcaag atttgccgcc acctcttcac ggacgtgttc
     4381 cgcgctggtc atgccgatga ataccgcttc aatagccaac agaccaccgg ggagtagcgg
     4441 gtagcatttc agggccatcg ccagagtgaa aataaattcc gctaccagca accagccagc
     4501 gacgaaaggg ctgatgagga aaattaacgg atttacgatt aagaaaatga tgagggcgag
     4561 tttgtaccag tcgggggact ggcccaaaaa attgcgccat agcgcgcggc cccaggagat
     4621 ctccatgatg gtttccctta ccttacaaat aatcaatgat gtttttatgt ttaaacgcaa
     4681 agcttaacgg tcaggcagga gtgaggcaag tcttgatagt caaggggaaa gagatgcgga
     4741 aaatgaagcc ttgatccctt ttttcttctt tttgtctgct atcagcgtag ttagccctct
     4801 ggtatgatga gtccaacttt gttttgctgt gttatggaaa tctcactatg gtcattaagg
     4861 cgcaaagccc ggcgggtttc gcggaagagt acattattga aagtatctgg aataaccgct
     4921 tccctcccgg gactattttg cccgcagaac gtgaactttc agaattaatt ggcgtaacgc
     4981 gtactacgtt acgtgaagtg ttacagcgtc tggcacgaga tggctggttg accattcaac
     5041 atggcaagcc gacgaaggtg aataatttct gggaaacctc tggtttaaat atccttgaaa
     5101 cactggcgcg actggatcac gaaagtgtgc cgcagcttat tgataatttg ctgtcggtgc
     5161 gtaccaatat ttccactatt tttattcgca ccgcgtttcg tcagcatccc gataaagcgc
     5221 aggaagtgct ggctaccgct aatgaagtgg ccgatcacgc cgatgccttt gccgagctgg
     5281 attacaacat attccgcggc ctggcgtttg cttccggcaa cccgatttac ggtctgatcc
     5341 ttaacgggat gaaagggctg tatacgcgca ttggtcgtca ctatttcgcc aatccggaag
     5401 cgcgcagtct ggcgctgggc ttctaccaca aactgtcggc gttgtgcagt gaaggcgcgc
     5461 acgatcaggt gtacgaaaca gtgcgtcgct atgggcatga gagtggcgag atttggcacc
     5521 ggatgcagaa aaatctgccg ggtgatttag ccattcaggg gcgataatcc cttccgttta
     5581 aaaagcaaac ccctcaaacg aggggttttt tgttgttttt acagatttcc cattcttggc
     5641 gggcaacgtt ccagcaactc gacgctgcca tcttcgtttt gctgttccag catcacatca
     5701 aatccccaca ggcgatgcac atgcttcagc acttctttgc gcccccgatc cagcggtgcg
     5761 cgattatgtg gaatgtaacg cagcgtcagc gaacggtcgc cgcgcaaatc cacgttccag
     5821 atctgaatat tcggctccag attacttaag ttatattgcg acgataaccg gttacggatc
     5881 tcccgataac cttcttcatt atgaatagcg gaaatctcca gataattatg ccgatcgtca
     5941 tccagcacgg tgaagaagcg gaaatcacgc atcactttcg gtgacagaaa ctggctgata
     6001 aaactctcat ctttgaaatc acgcatcgcg aaatgcagcg tttccagcca gtcggaaccg
     6061 gcgatatccg ggaaccagta tttgtcttct tccgttggcg actgacaaat ccgtttaata
     6121 tcctggaaca tggcgaaccc gagggcatac gggttgatgc cgctgtacca cgggctgtta
     6181 taggggggct ggaagaccac attggtgtgg ctgtgcaaaa actccagcat aaaacgttcc
     6241 gttactttcc cttcatcata cagatggtta aggatggtgt agtgccagaa ggtggcccag
     6301 ccttcgttca tcacctgagt ctgtttttgc ggataaaaat actggctcac cttacgcaca
     6361 atacgcagga tttcacgctg ccatgattcc agcagtggcg catttttctc cataaaatag
     6421 agcaggtttt cttgtggttc ggacggatag cggcgcgctt cagcaaccgt tttctcttcc
     6481 tcgcgcttcg gcagggtacg ccagagcata ttgacctgac tttgtagata ctcttcgcga
     6541 cttttctgcc gggctttctc ttcttgcagc gagatttttt gcgggcgttt gtaccggtcc
     6601 acgccgtagt tcatcagcgc gtggcacgag tccagaagcc gttctacttc atccacgcca
     6661 taacgttctt cgcactcggt aatatattta cgggcaaaaa tcagataatc gacaatcgaa
     6721 ctggcgtcgg tccaactgcg gaataagtaa ttgtttttaa agaaagagtt atgcccatag
     6781 caggcgtgtg ccatcaccag cgcttgcatg gtaatggtgt tctcttccat caggtaagcg
     6841 atacacgggt tagagttaat gacgatttca taggccagtc cttgctgacc gtgcttatac
     6901 agccgttcag tctcgataaa ctttttaccg aatgaccagt gcggatagtt aattggcatg
     6961 ccgacgctgg agtaggcatc catcatctgt tctgaggtta tcacttcaat ctggtgcggg
     7021 taggtatcca gccggtagag tttcgccacc cggtctatct ctgccagata aacatccagc
     7081 aggtcgaacg tccagtcggg tccatcgctc aaccgtgtgg tgtccttatt catagaatcg
     7141 atcgtcgcca tacgcgcacc tcattgttgt cggcgctctc tgtgtggagc acctcatttc
     7201 aagcatagaa cacctgttaa aaaccgcgtc gccggagaat ttttttcttt gcgatttctt
     7261 attatcagag tgccactaat ccgcttctga acggaatttt atgctggata aaaagggcgt
     7321 tcagcaggag atactaaaga cgccatattg ccgcagagtc agggagatgt gagccagctc
     7381 accataaaaa agctgtatgt tgaataatat tttcaactga gttatcaaga tgtaattaga
     7441 ttattattct tttactgtat ctaccgttat cggagtggct atgcgagttg tcatactggg
     7501 aagtggtgtg gtaggcgttg ccagcgcctg gtacttaaat caggcaggac atgaggtcac
     7561 cgtcattgat cgggagccgg gggccgccct ggaaaccagc gccgcgaatg ccgggcaaat
     7621 ctcccccgga tatgctgcac cgtgggcggc accaggtgtg cctttaaaag cgatcaaatg
     7681 gatgttccag cgccatgcgc cgctggcggt tcgtctcgac ggtacgcagt ttcagttgaa
     7741 atggatgtgg caaatgttac gtaactgcga caccagccac tacatggaaa acaaagggcg
     7801 gatggtgcgt ctggcggaat acagccgtga ttgcctgaaa gcattgcgcg ccgaaaccaa
     7861 tattcagtat gaagggcgtc agggtgggac gctgcaactg ttccgtaccg aacaacagta
     7921 tgaaaatgcg acccgcgata tcgccgtgct ggaagatgcc ggcgtaccgt atcagctgct
     7981 ggaatccagc cgcctggcgg aagtggagcc agcgctggca gaagtagcgc acaaactgac
     8041 gggtggtctg caactgccca atgatgaaac cggagactgc cagctgttta cccagaatct
     8101 ggcgcggatg gcagagcagg cgggggttaa attccgcttt aatacgcccg ttgaccaact
     8161 gctttgcgac ggcgagcaaa tctacggcgt gaagtgtggc gatgaagtga ttaaggccga
     8221 tgcgtatgtg atggcgtttg gttcttactc gacggcgatg ctcaaaggca ttgttgatat
     8281 tccggtgtat ccgctgaaag gctactcgct gactattcca attgcgcagg aagatggtgc
     8341 gccagtatcc accattcttg atgaaaccta caaaatcgcc attacccgtt tcgataaccg
     8401 cattcgtgtt ggcggaatgg cggaaattgt tggttttaac accgagctgt tgcaaccgcg
     8461 tcgtgaaacg ctggaaatgg tggttcgcga tctctatcca cgcggcggtc atgtcgagca
     8521 ggcgactttc tggacgggtt tgcgcccgat gacgccagac ggtacgccgg ttgtcgggcg
     8581 tacacgcttt aaaaatctgt ggctgaatac cggtcacggc acgctcggct ggacgatggc
     8641 ttgcggttcc ggtcagttgt taagcgatct gctctctggt cgcacgccag cgatcccata
     8701 tgaggatcta agcgtagcgc gctacagccg tggatttacg ccatcacgtc cgggccattt
     8761 acatggcgca cacagctaag gaaacgagat gacccgtccg atacaggcca gcctcgatct
     8821 gcaggcatta aaacagaatc tgtccattgt ccgccaggcc gcgccgcacg cgggtggtaa
     8881 aagcgaacgc ttacgggcat ggtattgagc gtatctggag cgcgctcggg gccaccgatg
     8941 gctttgcatt acttaacctg gaagaggcaa taacgttacg tgagcgcggc tggaaggggc
     9001 cgatcctgat gctggaagga tttttccatg ctcaggatct ggagatttat gaccagcacc
     9061 gcctgaccac ctgcgtacac agcaactggc agctcaaagc actgcaaaat gcgcggctaa
     9121 aagcaccgtt ggatatttat cttaaagtga acagtgggat gaatcggttg ggcttccagc
     9181 ccgatcgcgt gcttaccgtc tggcagcagt tgcgggcaat ggcgaatgtt ggcgagatga
     9241 ccctgatgtc gcattttgcc gaagcggaac atcctgatgg aatttccagc gcgatggcgc
     9301 gtattgagca ggcggcggaa gggctggagt gtcggcgttc gttgtccaat tcggcggcga
     9361 ctctgtggca cccggaagcg cattttgact gggttcggcc tggcattatt ttgtatggcg
     9421 cttcgccgtc cggtcagtgg cgtgatatcg ccaataccgg attacgtccg gtgatgacgc
     9481 taagcagtga gattattggt gtccagacgc taaaagcggg tgagcgtgtg ggctacggcg
     9541 gtcgctatac tgcgcgcgat gaacagcgaa tcggcattgt cgccgcaggg tacgccgacg
     9601 gttatccgcg ccacgcgcct accggtaccc ctgttttagt ggacggcgtg cgcaccatga
     9661 cggtggggac cgtctcgatg gatatgctgg cggtcgattt aacgccttgc ccgcaggcgg
     9721 gtattggtac gccggttgag ctgtggggca aggagatcaa aattgatgat gtcgccgccg
     9781 ctgccggaac ggtgggctat gagttgatgt gcgcgctggc gctacgcgtc ccggttgtga
     9841 cggtgtaact tgttgtaagc cggatcggag gcaacgtctt ctgggtgcaa aaaaaatcat
     9901 ccatccggct ggtcagcaac tgtagttgtt aatgtgacag agccattgcc catgatagtg
     9961 tccattaaaa ggatggacac tatttccccg gaacctgaac tcaccgcaca ggcgttctac
    10021 ataaaacgct tacgcttcat tgttgactcg gagtcgactt cagataaatc acgctttacc
    10081 cttgatggag cctgtacata gatttgtgta attgcctgat tttgatatgt tcaattcaac
    10141 atcaaatgaa ggttaaatta tggacgacaa acaattgcag gctcaggctg cgttcagcaa
    10201 agcatcgcaa ccggcgatag atgcttcatt aaatttaaga ttcagcttcc tcttcagcca
    10261 cccgtacgcc aatcttcaac acttcattat cttctttctc ggccaccgtc cagatcatcc
    10321 cggcaaactc tacctggtca ccgacaaccg gtgccgtgcc taacaactgc tggacaattt
    10381 cacccagcgt ttgctgctta tcacgatatt ctcgcccgtc ttccaggcca tatatcagcg
    10441 caacatcagc atatttggcg ctggcttcga gaatgaagtc accaaagaag cgttgatcca
    10501 gcgcgaccgg cggcgactgg ctgaacagtt taccgagcgc cggaagatcg cgttcccgac
    10561 caattacaca caacacatcg ccttcgcgca gtcgggtgct gccggtagga tgaagcaact
    10621 ggttatcacg aaacagtgcc gcaatacgcg tctcttttgg catatgcaaa tcgcgcagtg
    10681 ccgcgcccac gcaccattta tcggcactca attgataaac aaactgctcc cacggatttt
    10741 ccggatgaat atccaggcca acgcgtgaca ccggacgtcc cactggcgga accaccactt
    10801 tggctttttt agccgcccac gagagtgatg ttccctgcaa gagcagtgaa accagaacca
    10861 caaagaaggc gacattaaag aacagacgtg cattctccag ccccgccatc atcgggaaca
    10921 cggccaggat gattggcacc gcgccgcgta atcctaccca gctgataaac acgcgctcac
    10981 gcagattgaa accgcggaag gggagcaatc cagcaaatac cgaaagggga cgggcgaaga
    11041 atatcatcca tgccgacaaa attagcgccg gaatggcaat aggcagcaga tcgcttgggt
    11101 taaccaatag ccccagcacc aggaacatgg cgatttgcgc cagccaggcg aggccgtcga
    11161 aattttgcag gatgccgtag cggttgcgaa tcggccgatt acccagcaga aaaccgcaca
    11221 gataaacagc cagaataccg ctgccttcca gcgccgtggt taaagcaaaa attaaaatac
    11281 cgccgcttaa tgccagcaat ggatataatc cggcaggcag ggcgatgcga ttaatcattt
    11341 gcagcagtaa ataaccaccg ccaagtccga tgacaatccc gaggccaaat tgctgcagaa
    11401 tatcgaccac gaacatccaa ctgacactgc tttcatgttg ctggatcatc gcaatcaggg
    11461 taatcgtcag aaagaccgcc attggatcat tactgccgga ttcaatttcc agcgtcgagc
    11521 caacacgttc gttaagcccc ttaccaccca gcagagaaaa gaccgctgca gcatcggtag
    11581 agccgacgat agcgccgatt aataagcctt caatcaaatc aagattaaac agccacgccg
    11641 ccatcatgcc ggttaaacca gaggtgataa gcacgcccag cgtcgccagc gacagtgccg
    11701 gtcctaacgc cacacgaaag gaactggcct gagtgcgcat cccgccgtcg agcaaaataa
    11761 tcgccagtgc caggttactt accatgtagg cgaaggggta attatcaaac gggataccgc
    11821 cgacgccatc gactcctgcc agcatgccga tcgccagaaa aataaccaga ataggaatgc
    11881 caagacggga agaaaatgaa ctaagtaata tactgctggt gacaaggatg gatcctaaga
    11941 tgaaaaggct aattattgtt gtggcatcca acggtcggtt actcctgatt acgctgtctc
    12001 ttatataaac cctaccatat tagcggcaga gacagcgttt tacttagccc tgaagcgtga
    12061 tttttttaca ttttaagaac aggatgaccg ctaatcgtca actgagtgcc ttcccgggtg
    12121 ttattcagaa tggcatgtgc gcccaacggc agcgtaaccg tgcgttgttc atgaccaaaa
    12181 tcgaggccgg taatgagtgg aatcgacagg cgggaacgca aaaacgcgga cactgactcc
    12241 aggttgtaac ctgcgtcata atcattgggc gtgctgccgc taaagctacc gagaataatc
    12301 gccttctgac gtggcaaaat tcccgcatga tagagctgca acagcatacg ttcgacgcgg
    12361 aaagggtgct cgttaatatc ttccagtacc aggataccgt tctcaatttt tggcatccac
    12421 ggtgtaccaa tcagtgaaat cagcatcgca agattgcctc cccacaacgt gccttcggtc
    12481 tgacatgtcg ggccttcccc ttgccattca atggtgaagg ttttattgcg taacgccagc
    12541 cagaaatggt gctcggtaaa ggcgttcagt tcatccgcac caaagtttgc taccagcatg
    12601 gggccgctaa aggtgatgac attgccttgc gccagaagac cgcactgaat ggcggtaaaa
    12661 tcgctatgtc cgcaaatgag taacgggtca tgttgttggc gagccaccag tgcctgccag
    12721 tcaatatctg ccagtaaacg actggcaccg taaccgccgc gtacagccag cacgatggtg
    12781 ttgggtgtag tcagtctggc aagggaattg agatcttcca gacgctccgt ttccgtaccc
    12841 gcaaaacgct cacaacgacg ggcaataacc tcgacgttat tgacttgatg ccccgcgtcg
    12901 gtaaggcgct ggataccacg cagcgcggcg tgctgtttaa tgctgtaacc cgatggggca
    12961 attaagtgaa acagagacat ggcaattcct tgctgacaac agaaacgaaa tgtatatcat
    13021 gccgcttagg tgtgccgttg tcacctcaac ggcgattcca ggctataagg atagaagaag
    13081 tgaaattgag atggtttgcc tttttgattg tgttattagc gggttgttca tcaaagcatg
    13141 actatacgaa cccgccgtgg aacgcgaaag ttccggtgca acgtgcgatg cagtggatgc
    13201 caataagcca gaaagccggt gcagcctggg gcgtcgatcc acaattgatc acggcgatta
    13261 tcgctatcga atcgggtggt aatccgaacg cggtgagtaa atcgaatgcc attggtttga
    13321 tgcagttaaa agcttcaacc tccggacgtg atgtttatcg ccgtatgggc tggagtggtg
    13381 agccgacgac cagcgagctg aaaaatccag agcgtaatat ttcaatgggg gcggcttacc
    13441 tgaatattct ggaaaccggc ccgctggcag gcattgaaga tccgaaggta ctgcaatatg
    13501 cgctggtggt gtcatacgct aacggggcag gtgcgctgct acggactttc tcgtcagatc
    13561 ggaaaaaggc gatcagcaaa atcaacgatt tggatgctga cgagttcctc gatcacgtag
    13621 cgcgcaatca tcctgcgccg caggctccgc gctatatcta caaacttgag caggcactgg
    13681 acgcgatgta aatcagtcgc gtactttgtc cgctttctcc cgggcttccc gctcgagaga
    13741 gaaaataatc cgttgtaatt gccgctccac cgtcgggctg acgttaagaa aacggaagct
    13801 cagacgggga gtggtgatgg tttcattctt gccatcaatc actttgcgct cgctgatgga
    13861 gattaactgg gcgtcaaagt gaaaaacacc ccattgcccc atgttgactt caatctgagc
    13921 gaagcgcatg ccttcttgta attcggcagg ctttgctgtt tccagtaatg cgcccatgcc
    13981 gcctaacgac aaatcataca ggcggaaacg taacgtactg ttatccgcca gtttggtctg
    14041 gcaaaaataa ggcggatgga gtggggcgga gatgcggaaa tatcggcgtc gttgtacaaa
    14101 ccataaggtg ggaggcggta cggtaataaa tgccggaagc tgcaagtatt cactctgctg
    14161 tagttgttca acagtaaact cgactttcgc accctgagtt tcggcggtaa tggtaatatg
    14221 ctgcgccttt agcacggcga tgttgtcttc ggcttgactg ccgaaatcca gcaccagttt
    14281 atccggggtt attgccagta ttttgctgat cagctgcccg ccattccaac tgagacgcaa
    14341 aggaattgcg gctttgtgca aatcgcgtaa cacgcccagg acggctaacg gattttgttt
    14401 caggaactgc tcatggtaat gactcacgcg gaaaaactcc tgactgacaa actgtctttg
    14461 gtttatcggt cacagttaac aaaacttaat acaaatgcgt gaatattttt tacatgttgt
    14521 tcttaaatca gccgacatac gcccagcatc gatcccgccc ttgcctatac ttagagcgtt
    14581 gacgtaagca tttcttgcgt ccgattcatc gaaacgaggg cttgaacatg ggaattattg
    14641 cctggattat ttttggcctg atagccggca ttatcgccaa gctaatcatg ccggggcgtg
    14701 atggtggtgg atttttccag acctgtattc tcgggatagt cggtgcggtg gtcggcggct
    14761 ggctggcgac catgtttggc attggcggct ccatcagcgg ttttaatttg cacagcttcc
    14821 tggtggcggt ggtgggagct attctcgttc tgggcttatt ccgcctgctg cgaagagaat
    14881 aagattttca taaggcggat agcgatacag atgccgctat ccgcttttca catcagaacg
    14941 tatattcgac acctgcttta cgggtgaaaa aaatcaatat agcacttcga tatccgataa
    15001 agtcggttcc cggtaacaac aacagaatcc ttttttaatt attgtttcgt tgtttttggt
    15061 gtgattcaga aaataaatat tttagacagt agccagtcag gcgttaaaaa tggggcggat
    15121 agtgaggcgg ttttggctgc gcttattgaa catttagaat tgatcaatcc ttctggcaga
    15181 ttaagccaaa atacccgttc ggcaatgctt cagttacgcg aagaggaatg gtctgaattc
    15241 tttttttggt tgctaaattc tctggagtgt ctggattatg tgattattaa tttaactcca
    15301 gaaagcaaaa aaacgttgat gagtgaacac cgtaacaata ttcaggtagc aatcgatgct
    15361 ttatataggc aacgtcggtg taaatcaccg ggggatgata gtgaaacact tacccgtaga
    15421 aatgacgcta tcttcggcaa ccacgtatgg caaacatttg cgcaatactt cccgccagga
    15481 cttgaaaaac cggctgtgta gaaaacacca tagctatggt gttatctgcc gacaacgcca
    15541 tccggagaag ccgaatgacg ttgtcggagt aagcgctggt taaggtgtgg gttgtgcctc
    15601 tttggttgag ggttgcgtcg ttgctgactt aacggtcgga cgcgtcgccg gaacattgtc
    15661 acacggttgc tctttcgggc agatcaaatc cagcattttc agcgtcacgc cattggtcca
    15721 gccaaagcca tcctgtaatg gatattcgcc acctcttttt gcccgtagtt ttgtaatcct
    15781 tctgtcgcga cccactgtaa cggtgcccag ccatttggcg catcccattg ttgcccactt
    15841 ttcaccgacg tggtgttcag gccgccgggt tgcagcagat gtgttttcgt cgccgtcgcc
    15901 attttgttgg cgcgatcttt cgctgccgca ttgacgtaca gcgggaacag ggcggccgcg
    15961 gttaactgat tgcgcacttt atgacttttc aggtcgtaat cggcatacca gccttgttga
    16021 tcgttccaca ggtatttttc gattcctttt tgacgggcat tcgccagcgt ttcgtactgg
    16081 tttgccatcg cgttatctcc tgcagctttg ctggcgcggg cgaggatttt ttccatttta
    16141 aacatcaggc tgttcagatc gaccggtacg atgctggtgg tgcgtaaggt atttaactgc
    16201 tgcgggttgt ccatccagcg cgagctgaaa tcccagccag acgcagcggc agagcgcagg
    16261 tcgcggtaaa tttcagtggc aggtcgattc ggattgcttt tggcggtggc aatatcttcc
    16321 acccatgact ctggtcgtgg cgtatcgcga tcgtcccagt agcggttgag aagggtacca
    16381 tcctgaagtt tgacaacgcg tttttcctgt tgtccggctt gcaggttttc aacaccgtcc
    16441 atccagtaag catattcttt ttgcatttgc ggcaggtatt gcttcaacgc ggcatcgcct
    16501 tcatgctgcg ccagtaactc taccatcagg gcaaagaagg gcggttgcga gcggcttaaa
    16561 tagtaactgc ggttgccgtt gggaatatga ccgtaagtgt ctatttcatg agcaaaattg
    16621 gccaccatat ccgcgacttt atcccagtga ccgctttcag caagtcctaa catggtgaag
    16681 taactgtccc agtaatatac ctcgcgaaaa cgtccgcccg gcacgacata aggttccggc
    16741 agcggtaaca gagaatccca tttttcggtg ttttcggtag aacgcgttaa taccggccaa
    16801 agtccgtcaa tatgttcgcg cagtgactgc ccctctggcg gaacatattt ctcgccttct
    16861 ttcggcaggg tgaaattgac gttaacgaaa tggcgcagat caaatccgct ctggttttgc
    16921 tgcatccgat aatcagcgga tcgctgttcg gcacggcatc ggcaaaggtt ttttggtccg
    16981 gaaaaagttt ggcgttttgc acatcattaa acagcggccc taataaaata tcaggcggct
    17041 atggtgttac cggtgtttct tctgcctgca ccgatagcgc agcgaaacac aaaaagatac
    17101 aggct
//
LOCUS       DAJTSL010000082        17010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTSL010000082 DAJTSL010000000
VERSION     DAJTSL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.082"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            177..614
                     /locus_tag="OO433_002421"
     CDS             177..614
                     /locus_tag="OO433_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCR6050866.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLADILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDSYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     gene            748..1292
                     /gene="cobU"
                     /locus_tag="OO433_002422"
                     /pseudo
     CDS             748..1292
                     /gene="cobU"
                     /locus_tag="OO433_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
     gene            1289..2032
                     /gene="cobS"
                     /locus_tag="OO433_002423"
     CDS             1289..2032
                     /gene="cobS"
                     /locus_tag="OO433_002423"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6050867.1"
                     /translation="MSKLFWTMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILALLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            2044..3123
                     /gene="cobT"
                     /locus_tag="OO433_002424"
     CDS             2044..3123
                     /gene="cobT"
                     /locus_tag="OO433_002424"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6050868.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGGAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            3185..4120
                     /gene="ldtA"
                     /locus_tag="OO433_002425"
     CDS             3185..4120
                     /gene="ldtA"
                     /locus_tag="OO433_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6050869.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSYTVTVP
                     DHNTQPLETFAAQNGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYEFDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVPISSGSRQMF"
     gene            complement(4176..4251)
                     /locus_tag="OO433_002426"
     tRNA            complement(4176..4251)
                     /locus_tag="OO433_002426"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4216..4218),aa:Asn,seq:gtt)
     gene            4578..4949
                     /locus_tag="OO433_002427"
                     /pseudo
     CDS             4578..4949
                     /locus_tag="OO433_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4948..5382
                     /locus_tag="OO433_002428"
                     /pseudo
     CDS             4948..5382
                     /locus_tag="OO433_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
     gene            5725..6441
                     /locus_tag="OO433_002429"
     CDS             5725..6441
                     /locus_tag="OO433_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6050870.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(6897..6972)
                     /locus_tag="OO433_002430"
     tRNA            complement(6897..6972)
                     /locus_tag="OO433_002430"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6937..6939),aa:Asn,seq:gtt)
     gene            complement(7073..8716)
                     /gene="yeeO"
                     /locus_tag="OO433_002431"
     CDS             complement(7073..8716)
                     /gene="yeeO"
                     /locus_tag="OO433_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCR6050871.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKVLALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWGLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            8721..8796
                     /locus_tag="OO433_002432"
     tRNA            8721..8796
                     /locus_tag="OO433_002432"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8754..8756,aa:Asn,seq:gtt)
     gene            8958..10220
                     /locus_tag="OO433_002433"
     CDS             8958..10220
                     /locus_tag="OO433_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6050872.1"
                     /translation="MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRF
                     SGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNINPAHQRMSDKAAASPEKYFKAVA
                     LAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEV
                     ASRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGD
                     YQQGRQLTRLAVVLTLHLFIRSSELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRG
                     AKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMSENTVNRALRLMGYD
                     TKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAM
                     MQWWSDYLDVSREGYVAPYIYARRHKAA"
     gene            complement(10932..11816)
                     /locus_tag="OO433_002434"
     CDS             complement(10932..11816)
                     /locus_tag="OO433_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HCR6050873.1"
                     /translation="MGILEQEMKRLAQQAGGSHKTVHDRIKLVQRFCERLVLAQNVQI
                     RRVEQLKARYIEGYVRERLAQGITKRSLQNEMAAVRCILKQAGRDRLAQSERLNNRSL
                     GLSGASRNGTKRAITPEHYRYVLETARAKDPGMAAALELSRLMGLRSQEVVQSAQSLR
                     TWKQSLERGEPRLTVVFGTKGGRPRETVILDAVAIRKALDNALVVAEDRHGRLIDKPD
                     LKSAMKYWHSQASRLGLTGTYSPHSLRYAWAQDAIRHYLAQGFSEKEALAMTAMDLGH
                     GDGRGRYVAQVYGRKDTD"
     gene            12375..12521
                     /locus_tag="OO433_002435"
     CDS             12375..12521
                     /locus_tag="OO433_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050874.1"
                     /translation="MYRIKTLASVAMFCDPVFAEVCTGKRIDRPDNRRFFQVVKRWAL
                     STTA"
     gene            complement(12681..12998)
                     /locus_tag="OO433_002436"
     CDS             complement(12681..12998)
                     /locus_tag="OO433_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6050875.1"
                     /translation="MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVP
                     VTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTIDMGARSGKRLERIPDAVVNEVL
                     ARL"
     gene            complement(12999..13256)
                     /locus_tag="OO433_002437"
     CDS             complement(12999..13256)
                     /locus_tag="OO433_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR6050876.1"
                     /translation="MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLII
                     EPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIL"
     gene            complement(13605..13811)
                     /locus_tag="OO433_002438"
     CDS             complement(13605..13811)
                     /locus_tag="OO433_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003842122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6050877.1"
                     /translation="MMLQDWHPADIIAGLKKRGTSLSALSRQAGLASSTLANALTRRW
                     PKGERLIAEALGIKPEQIWPSRYR"
     gene            complement(13808..14269)
                     /locus_tag="OO433_002439"
     CDS             complement(13808..14269)
                     /locus_tag="OO433_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050878.1"
                     /translation="MATIPTQTHPLLAVPFSAATDFTVLADHCENVAETLIESEDPVL
                     RLALCGRLAACLTLLQPTLNDPIPSHLTDSLTVDALPADMPRFVPDAELLCDYSLTLA
                     QLLIGQGMQPKVEQTITGLLCELVWYFAAEMKAPRWLRTADGVKFIDEVAA"
     gene            complement(14640..16244)
                     /locus_tag="OO433_002440"
     CDS             complement(14640..16244)
                     /locus_tag="OO433_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050879.1"
                     /translation="MITKELVDESNFISQLVDIGRFDESYQQSVAFLNKLERITIRND
                     NYFIVLANIAGALVDIGQMQNNKNASELGCNLMEDNKEAFISVLGECLYYYNYGNALS
                     NLVSITNPNDHTFKSIEELVSLKNIYWRSFKLSAEEQEEFQAELSVNLANSLRSQFRL
                     SESLRYYDLTNLKGLDIPQAWVNRSMSLMVLNLISSSFSIKLLKEVRAGYIKASVSKN
                     IPPQWESFYLERIAQTNEKIAEYAVDDETDEHDEALTQQEFEVLSPYRQFCLRNHLTL
                     SEHGLYCPCVGSATDDLVIASRGGVTGDFIIPMEMVLNRLKSEFSLARHLYFDYLHPQ
                     NTDYIKYESHFLELYNDEVLGIEIEKIRTAFRLCFGILDKIAVAICELYNLYPTTKKG
                     TPQKNIYFQNFWQLDVDNRRQLFEKVKSPGLLALYSIATDLNKDKGGELAFYKEWRNG
                     LEHKFLVVHKSDKPEDIYESYQLIEDILFIKESDFIHHFEHLLQITRSAIFSFAFMVR
                     QEGMKEKKEGIHYMTRELHAINYSAD"
     gene            complement(16257..16622)
                     /locus_tag="OO433_002441"
     CDS             complement(16257..16622)
                     /locus_tag="OO433_002441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050880.1"
                     /translation="MVSDKRADIIVSLPQIKIPIEIKRDYHRDVWTALNGQLDKLYTK
                     NPDAAGHGIYLVFWFGSARPNSLPHLAKNTSQPENASAMENMLNETVPVDKRDRLSAI
                     VIDVSCEGIPPVEAPKSSV"
     gene            complement(16650..16859)
                     /locus_tag="OO433_002442"
     CDS             complement(16650..16859)
                     /locus_tag="OO433_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050881.1"
                     /translation="MNKTPANIMDLYSLLLDHIRDISNRIANENTDIYKQFWNEDSYG
                     RTLTPKPEESCRNIFLDLLRVRLNP"
BASE COUNT         4479 a         4013 c         4080 g         4438 t
ORIGIN      
        1 aatttctttt tttcattgga gcacctgtgt tgttctgagg tgagcatatc acctctgttc
       61 aggtggccaa attcagtgtg ccacttcacc agggctgcat tcaaggtgtg gcaatattgt
      121 ttcatggctt atcaaccaga gcgccattac aggaaattaa gtggagggcg agaggcatga
      181 aaacgataaa acccgaatcg atagctcttt tttgcctctt atctggagat gtggatctgg
      241 cgaaacatct ggctgatata ttgccaataa cctgttttgt tggtgaggac tggcagcaag
      301 atttccagcc tcttgatggt gggcctgtgc aatcgttatc agattcgtat agcgattatt
      361 cagtgctgct attgatagca gctgaaagcg agatatccgt accagaatgg ctcgctcatt
      421 ctgagggaac gacggtaatt ttgattgcag aacacaatgc ccgtttgctg actggtccgg
      481 ctggtggggc tgtagcatta atccattatc tgaatgaaat ctgtcattcc agagaaaccg
      541 ctgtcaatga cgcattttgt cggacgttaa cacgaggaag tctggctatt ccgcaatgcc
      601 atcttctttc gtaatggatt gagaatgctg gatttttaat tatattacgc gcagtattaa
      661 tcgcgtaaat gttttgcggt cagcaacggt gggggatcca ttgttctgtt caatgaatac
      721 catcatacaa ttgaatcagg agtcggtatg atgatttcgg tgacgggggg catacggagc
      781 gggaagagtc gccacgcaga ggcgcttatt ggggactctt cacaggttct gtatacgcca
      841 cctcgcaaat ccttgatgat gagatggctg cacggataga acatcatcgg caaggccgcc
      901 cgacgcactg gcacacagtg gagcgctggc aacatcttga tgaattaatt catgcagaca
      961 ttaacccgca tgaggctgta ttgctggaat gcgttaccac catggtgact aatctgttgt
     1021 ttgattatgg cggcgataaa gaccctgatg aatgggatta tcaggcgatg gaacaggcga
     1081 ttaatgctga gattcagtcg ttgattgctg cctgccaacg ttgtcccgca aaggttgtat
     1141 tagtgactaa cgaagtggga atggggattg tgccggagag tcgtctggca cgacattttc
     1201 gtgatattgc cgggcgggta aatcagcagc tggccgctgc ggcaaatgaa gtatggttgg
     1261 tggtttcggg tattggagta aaaatcaaat gagtaaatta ttttggacga tgctctcttt
     1321 tattacgcgt ctgcctgtac cgcgtcgctg gtcccaggga ctggatttcg agcattattc
     1381 tcgcggtatt attacttttc ctttaattgg attattgctt ggcgcgatta gcgggctggt
     1441 cttcatggtg ctgcaggcat ggtgtggcgt accactggcg gcactgttta gcgtacttgt
     1501 gctggcgctg atgaccgggg gattccacct ggacggcctc gccgatacct gtgatggcgt
     1561 attttctgca cgtagccgcg atcgcatgtt ggaaatcatg cgtgatagcc gtttaggcac
     1621 ccacggcggt ctggcattga tttttgttgt actggcaaag attctggtat taagcgagtt
     1681 ggctctgcgt ggcgaaccga tccttgcatt gctggcggca gcatgtgcgg tcagccgcgg
     1741 gactgccgca ttattgatgt atcgtcatcg ttatgcccgg gaagaaggac ttggcaatgt
     1801 gttcattggc aaaattgatg ggcgacaaac ctgcgtcaca ctcggcttag ccgctatttt
     1861 tgctgccgta ttattgccgg gtatgcatgg tgtggctgct atggtggtga cgatggtggc
     1921 gattttcatc ctcggtcagc ttctcaaacg tacgctgggc gggcaaaccg gcgatacgct
     1981 gggagctgct atagagcttg gtgaactggt cttcctgctg gcactgctat aaagagacgt
     2041 gacattcaaa cacttgccga tttactgaat acgatccctg ctatcgatcc tgccgctatg
     2101 tcgcgtgcac aacggcatat cgacgggtta ctcaaacctg ttggtagcct gggaaggctg
     2161 gaggcgcttg ccatacaact ggcaggaatg ccggggttga atggcgttcc gcatgtgggc
     2221 aaaaaagcgg tactggtcat gtgtgccgat cacggcgtct gggaggaagg gggcgctatt
     2281 tccccaaaag aagtgacagc cattcaggct gaaaatatga cccgcggaac aaccggcgtg
     2341 tgtgtgctgg cagcacaagc gggcgctaac gtctacgtaa ttgatgttgg tattgatact
     2401 gctgagccta tccccgggct tatcaacatg cgtgttgctc gaggtagtgg caatattgct
     2461 tcagctccgg caatgagtcg ccatcaggca gaaaagttgc ttttggacgt catatgttat
     2521 acgcgggagc tggcaaaaaa cggtgttacg ctgtttggtg taggtgaact ggggatggca
     2581 aacacgacac cggcagcggc aatagtcagc acaatcactg gccgggctcc tgaagaagtg
     2641 gttgggattg gcgcaaacct gccgacagat aaactggcta ataaaattga tgttgtgcgc
     2701 cgggcgatta cgttgaatca accaaatccg caggatggcg ttgatgtcct ggcaaaagtg
     2761 ggtggatttg atttggtcgg aatggctggc gtgatgttag gcgctgcgtc ctgcggttta
     2821 cccgtgttgc tggatggatt tctttcttat gctgctgcgc tcgcagcctg tcagatgtct
     2881 cctgcaatca aaccgtatct cattccttct cacctgtcgg cagaaaaagg agcgcgtata
     2941 gcgctgtcgc atttggggct ggagccttat ctcaatatgg agatgcgttt aggtgagggg
     3001 agtggcgcag ctctggcgat gcccatcatc gaagctgcct gtgcgatata taacaacatg
     3061 ggcgaacttg ctgccagtaa tattgttcta ccggggaata cgacttctga tttgaacagt
     3121 taaaaacgaa aagtctgata aaagctatac ttaacggata gctttcgcga cttaggaaag
     3181 ggacatgatg cgtcgtgtaa atattttttg ctcatttgct ctgctttttg ccagccatac
     3241 tagcctggcg gtaacttacc cattacctcc agagggtagc cgtttagtgg ggcagtcgta
     3301 tactgtaact gttcctgatc acaataccca gccgctggag acttttgccg cacaaaacgg
     3361 gcaagggtta agtaacatgc tggaagcgaa cccgggtgct gatgttttct tgccgaagtc
     3421 cggctcgcaa ctcaccattc cgcagcaact gattttgccc gccactgttc gtaaagggat
     3481 tgtggttaac gtcgctgaga tgcgtcttta ttactaccca ccagacagta atactgtgga
     3541 agtctttcct attggtatcg gccaggctgg gcgagaaacc ccgcgtaact gggtgactac
     3601 cgttgaacgt aaacaagaag ctccaacctg gacgccaacg ccgaacactc ggcgcgaata
     3661 tgcgaaacga ggggagagtt tgcccgcatt tgttcctgcg ggccccgata atcccatggg
     3721 gctgtacgcg atttatattg gcaggttgta tgccatccat ggtaccaatg ccaattttgg
     3781 tattgggcta cgggtaagtc agggctgtat tcgtctgcgc aatgacgata tcaaatatct
     3841 gtttgataat gttcctgttg ggacgcgtgt gcaaattatc gaccagccag taaaatacac
     3901 cactgaacca gatggctcga actggctgga agttcatgag ccgttgtcgc gcaatcgtgc
     3961 agaatatgag tttgaccgaa aagtgccatt gccggtaacc ccatctttgc gggcgtttat
     4021 cagcgggcaa gaagttgatg taaatcgcgc aaatgctgcg ttgcaacgtc gatcgggaat
     4081 gcctgtgcca attagttctg gttcaagaca gatgttttaa gagcgttggt aattagaaag
     4141 caaaaagcct gctagaaagc aggctttttt gaatttggct cctctgactg gactcgaacc
     4201 agtgacatac ggattaacag tccgccgttc taccgactga actacagagg aatcgtgtga
     4261 acggggcgca tattaatggc agtgcatggt gatgtcaaag ggtgaaatgc atttttttgt
     4321 ttgtttgctg tcaatttcaa caaagtggtg agctttcaat cttattgggt aatgaaccat
     4381 cgtggtgcat accctccttt tatagggcag gggaacgcga cagctgatta aaggagcaaa
     4441 tgacgaatct atcataatct cttatttgac agttggttag cttgtacatc aacgccaaaa
     4501 taaaactggc aagcatcttg caatctggtt gtaagtaatg gcggcacttg ggccgattct
     4561 taaaaaccgg aggcaacatg aacttcagac gcctgaaata cttcgtaaaa attgtagata
     4621 ttggtagcct gacccaggct gctgaagtat tgcatatcgc acaaccagcg ctcagccagc
     4681 aggttgccac actggaaggt gagttaaatc aacaactttt gatccgtaca aagcggggcg
     4741 ttacgccaac agacgccgga aaaattctct atacccatgc gcgggccatt ttacgtcagt
     4801 gtgaacaggc ccaactggcg gtgcataacg ttggtcaggc attatcgggg caagtctcga
     4861 ttggctttgc accaggaacc gctgcgtcat ccatcaccat gcccttatta caggcggttc
     4921 gcgctgaatt tccggagatc gttatctatc ccgagcattg ctgaagtgca acgtgcgctt
     4981 tatgacgcca ccaagctggt gagcggcagg cccggtgagg aagtcaaaca gcggctacgt
     5041 actggtactg tggtaaccac agatgacagg aactgggaat tacgttactc agcttctgca
     5101 cttcgtttta acttaagccg ggccgtagca attgatatgg aaagtgcaac cattgccgcg
     5161 caaggatatc gtttccgcgt gccatacggg acactactgt gtgtttcaga taaaccgttg
     5221 catggcgaga ttaaacttcc cggccaggct aaccgttttt atgaaggcgc tatttccgaa
     5281 catctgcaaa ttggcattcg ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat
     5341 tcgcgtaaat tacgaacctt taatgagccg ccgttccgat aataagttct gaaccttctt
     5401 atcagaaata gatgtgaagg agtaagtaag acacctggca aatagcctgc aataatcgtg
     5461 ggctgtttgc ttccttgggc ggatacgtgt tttattatcg tcttaatgat ttccacatat
     5521 taaaagcaag tatgctttca aaacacaatt ataaaaaatc ccgccaacaa tataagtttt
     5581 tataaaatta aatataagat tatggcttta gaatattttt atttctaata gacgagattt
     5641 ttcctgttat gatataatat gccgaattaa cacatgttaa cgatttacca gtaatgtaaa
     5701 taaattttcg aggagatcat tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa
     5761 acggctaaag acggtgcaac gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct
     5821 gctgctaaac aaggtgaacc cgatccagaa ttaaacacat ctttaaaatt cgttattgaa
     5881 cgtgcaaagc aggcacaagt tccaaagcac gttattgata aagcaattga taaagccaaa
     5941 ggcggcggag atgaaacgtt cgtgcaggga cgttatgaag gctttggtcc taatggctca
     6001 atgattatcg ccgagacatt gacttcaaat gttaaccgta cgattgctaa cgttcgcaca
     6061 attttcaata aaaaaggcgg caatatcgga gcggcaggtt ctgtcagcta tatgtttgac
     6121 aatactggtg tgattgtatt taaaggatca gaccctgacc atatttttga aattttactt
     6181 gaagctgaag ttgatgttcg tgatgtgact gaagaagaag gtaacattgt tatttatacc
     6241 gaacctactg accttcataa aggaatcgcg gctctaaaag cagctggaat cactgagttc
     6301 tcaacaacag aattagaaat gattgctcaa tctgaagttg agctttcccc agaagattta
     6361 gaaatctttg aagggcttgt tgatgccctt gaagatgacg acgatgtaca aaaagtttat
     6421 cataacgtcg caaatctcta attatatatt aaagaaatct gtctttatgg cagatttctt
     6481 taatctcata taatccttat aaaaaatata atgttcaact cgtcatattg cttataaaaa
     6541 atcctcgttc ccagagaaat aatatttatt ataattccag ttcttctttt tctgattaca
     6601 gaaggcaaag tgacaattac gcatagtttc ccgataaaga cgcgacagct acatcccgca
     6661 taaggcattt ttctctttat ccttgtacgg tacttcatgg aacagagttt ttgaccttgc
     6721 gaatcgtgat gtctgttggg gaggggcaat ttgctcactg aagcgtgaga ctcgattaag
     6781 cgcacgaaac acagaaatca aaaaacccgg gcactttttt acaaggtaac cgggtaaaaa
     6841 taatttttat tttttaactg ttttgagact catagagatg tctcaaaact aaaatttggc
     6901 tcctctgact ggacttgaac cagtgacata cggattaaca gtccgccgtt ctaccgactg
     6961 aactacagag gaatcgtgag aacgaggcga atattagcga tgcccaccca caatgtcaaa
     7021 gcctgttatt taaatttgaa atcgtttgct gaaataatct gcattttgtc gtttattccg
     7081 acacaactgg ctttttttca cacttttgcg gttcggatcg agggtatttc cacagccaac
     7141 gtccagtaac cattcgccag taaaacagca cggcccgcac agcccagtcg gcaaacattc
     7201 ccatccagac accaaccaca ccccagccaa gcatgatgcc cagcacataa ccgacgacaa
     7261 cccggcaacc ccacatgctc aacatagaaa cccacatggc gtaacgggca tcgcgagcac
     7321 ctttaaatcc agcgggtagc ccccatgagg cggaccaaat aggcataaat aaagcattta
     7381 gccaaatcag aatcacaacg acatgtttaa cctgtggatc ctgggtgtaa aacgatgcca
     7441 taaccccggc aaagggagcc gttagccagg cgatggccgt taatccaaga gtggaaagcc
     7501 agaacacatg ccgcaactga atctctgctt gcgctatctg ccctaccccc aaccttcggc
     7561 ctgtaatgat cgtagaagca gagccgagcg cacttccggg taagttgata agagccgcaa
     7621 ttgaaaacgc gataaaattt ccggcaataa cactggtccc catcccggca acgaacattt
     7681 gggttaataa ccgaccactg gtaaataaca ctgattcgac actcgcggga ataccaatcc
     7741 ccatgacttc ccagataatg ctaaaattca gcggtttaaa atagctcttt aacgaaatcc
     7801 ttagcgcagg attaaaacca atcgccagca cccacaaaat tgcaactgcg ccaatataac
     7861 gagaaatggt taaacccagc cctgccccga caaatcccag tcccggccag gagaaaaggc
     7921 cgtaaatcaa tatgccgcta ataataatat taagaatatt caggctaccg ttaatcaata
     7981 gcggtatttt cgtattccct gcaccacgaa gtgccccgct accaataaga gtgatggcag
     8041 ctgctggata actgagtacc gtcagctcca gataagtcaa cgccagtact ttaacttctg
     8101 tcgtggcatc acccgcgacg aaatcaataa tttgttcgcc aaaatgatga ataagcgttg
     8161 ccaacagtac ggcaaacaac gtcatgatca ccaatgactg ccgcgtcgcc accctcgctc
     8221 gtcgtcgatc ccgcttaccg agactaaatg ccacaacgac agtagtacca agatcgatag
     8281 cagcaaaaaa agccataatg accatattga agctgtccgc caatcccacg ccggccatcg
     8341 catcttttcc cagccagctg accagaaaag tgctcagaac ccccatcaac aggacacagg
     8401 cattctccat gaagatagga acagcaagcg gggttatctc gcgccagaac aacactttgt
     8461 agctcttgcg tttagcgtgc cagcgagtgc cgtgaacaac ctggcgtaaa gcagaggaga
     8521 tattcaaagc cgaccttaat tgcagaaagt gaaaccacat ttcaaataat gagggagaat
     8581 cagcaaagct gcaaagattt tcgccaacaa attgtctgca aatgcaacaa actgttgata
     8641 gaaacggcaa acagttgggg aatttaaaaa tcgggtttga caaaagattt ttcgccgtta
     8701 agatgtgcct caacagcgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
     8761 cgtatgtcac tggttcgagt ccagtcagag gagccaaatt ccgattctta cgctatctta
     8821 ttagtcctta tttgatattg attcaataag ttagcgtgaa aatctttcca gatgtgtatt
     8881 cagttttccc tccgcatccg gttaatttgg tggtcagatt tgggggcagg ttggttcgat
     8941 tctggagtga cccccaaatg tctttaaacg attcaaaaat ccgtaactta aaaccctctt
     9001 caaaacccgt aaaactgtct gattcgcacg gtctgtatct gctggtcaat cagggcggtt
     9061 cgcgtatctg gtatctcaag tatcgcttca gtggtaaaga gtcccgcgtc agtctgggcg
     9121 catatcctct ggtttcactt gctgaagcaa gacaacgacg tgacgatatc cgtaagctac
     9181 tgacgcagaa tattaacccc gcccaccagc gcatgtctga taaagccgca gcgtcaccgg
     9241 aaaagtattt taaagccgtg gcgcttgcat ggcacaaaac caacaaaaaa tggtcgcctg
     9301 attatgccgc gcgcatcctc gccagcatgg aaaatcatat ctttccggcg attggtcatc
     9361 tgcctgtcac tacgctgaaa acgcagaatt tcactgcgtt actgcgggtt attgaggata
     9421 aaggttttct ggaagtcgcg tcccgaaccc ggcagcaact ctgcaacatc atgcgttacg
     9481 ccgtacagca gggattcacc gaaaacaacc tggcgcagca tctggaaggc gtcaccgcgc
     9541 cgccggttaa gaatcactat cccgccctgc cgctggagcg gttgcccgaa ttgtttgagc
     9601 gcattggcga ctatcagcag ggacggcagc ttacgaggct ggcggtggtg ttgactctcc
     9661 acctgtttat ccgttccagc gagctgcgct ttgcccgctg gggtgagata gatttcagaa
     9721 acaaaatctg gaccatcccc gccacaaggg aagcgattga gaaagtccgt ttctctggcc
     9781 gtggcgcaaa aatgcgtact ccatatatag tgccgctttc tcgtcaggct atcgccattc
     9841 tgaagcagat aaaggaaatc tccggccatc tggaactggt gttccccggc gaccataacc
     9901 cgtataagcc gatgagcgaa aacactgtca accgggcgtt gcgtcttatg ggctatgata
     9961 ccaaaactga tgtctgtggg catggattcc ggacaatggc gtgtagcgca ttagtggaaa
    10021 ctgaactctg gtcacgggat accgtggagc ggcagatgag tcatcaggag cgcaacagcg
    10081 ttcgggcagc gtatatccac cgggctgaac atctcgacgc ccgtacagcc atgatgcagt
    10141 ggtggtcgga ttatctggat gtaagtcgcg aggggtacgt tgcgccgtat atttatgcgc
    10201 ggcggcataa ggcggcctga gacagtggct atataatttg ttaaaaatga aaaagcagat
    10261 cctgattgaa tccggatctg ctttttacat taggcaatcg ctgccagaca atctgcacca
    10321 gaaacgccat aactgcacca gttcagtacc accgcttacc tgcgccagtc tgccgtcgcc
    10381 tgtctgtgtc aggactgcgc cagatcttat cgaataaaca tcaaactgcg ccagtgttac
    10441 ggccctttca gccctttggc cttttccggt ctggcgctgt tacccttagc cgccccttac
    10501 cctttccggc ctttttcctt tgctggcgca atcgccagaa aaatcgtcaa gctgcaccag
    10561 aagcctgcca ataggtcata ccgttcagaa gcatcgttat tgagaggaaa ccgtacaaaa
    10621 aatttctacg tagaaatttt ttcggtggat aacgatgaat aaagagtatt ttataatgtg
    10681 atggctatca aaaatcgacc atccaaaaat aattcattga tttataatga ataaccatga
    10741 atttaatagt cgatgattat tgtttttatc agtattgata ggaaagtata caaaaaattt
    10801 tggccccaaa attttttgta cgaaaaccga tgaataacgc gaaattaata ggaaaccatc
    10861 caaaattatt tggggcaaat aattttgccc gaaaaccgat ggataactcg atcttttcaa
    10921 agcaacggct atcaatcagt atcctttctt ccgtaaacct gcgccacata ccgcccgcgc
    10981 ccgtcgccgt gtcccaaatc catcgccgtc attgccagcg cttctttctc actgaatccc
    11041 tgcgccagat aatgacgaat ggcatcctgc gcccacgcat agcgcaacga gtgcggggaa
    11101 tatgtgccag ttaacccgag acgtgacgcc tggctgtgcc agtatttcat cgcgcttttc
    11161 aggtcgggtt tatctatcag cctgccgtgg cgatcttctg ccacaaccaa cgcattatcc
    11221 agcgcttttc tgatagcgac agcatccaga ataacggttt ctcgcggacg cccgcctttg
    11281 gtgccgaaca ccacggttaa acggggctca ccccgctcca gcgattgctt ccacgttctt
    11341 aatgactgcg cactttgcac cacctcctgc gaacgtaatc ccatcagcct tgagagttcc
    11401 agcgccgctg ccatccccgg atcttttgcg cgggcggttt ccagcacata gcggtaatgc
    11461 tccggcgtga tggcccgctt cgtaccgtta cgggatgcgc cagacaggcc cagcgagcgg
    11521 ttattcagtc gctcactctg cgccagcctg tcacgcccgg cctgtttcag aatgcagcgc
    11581 accgccgcca tctcattttg cagggaacgc ttcgtgatac cctgcgccag tcgttcacga
    11641 acatatccct caatataccg cgccttaagc tgctccaccc ggcggatctg cacattctgt
    11701 gccagaacca gacgctcaca aaaccgctgc accagcttaa tacggtcgtg aacggtctta
    11761 tggctgccgc ccgcctgttg tgccaatcgc ttcatttcct gctccagaat ccccatacat
    11821 tccccctttc atgattgccg caaactcatc cccaaaaact caacactgat acagcgttcc
    11881 ccggcgcaca ggccgatagc ccctgagttt tgtcggggct gcgaacgata cctgagcgcc
    11941 ggggcggcag ccgttgaggt gtggtggggc ttcggttgtg ctctcagagc agccgcccgc
    12001 ttgcgcgggt catcccccgg atcgtccaga tccgcctcgg tatcggttca tattctcctt
    12061 atgggtaaat ggcctaaaaa ggcggtgtca gaatccagcc atgagctggc gcagttgagt
    12121 tttctggtgc aggtcgaaag ggcttgcgtg cgtcactttc ccgctttgcg cggttaaagt
    12181 gacgcacgga tctggtgcag catctgagat ttatcgtctg gtgcagtttt acggtcacgc
    12241 ggcagcaaag cctgacggca atgcaggccg cagcccgtaa ggtaaaaggc gctgagggtg
    12301 tcagcagatg caggttagcc gggagaattc cggcgcggtg cagattcaga ccagcaggct
    12361 ggcgaagtaa aaggatgtat cgtattaaaa ctctggcctc cgtcgcgatg ttctgcgacc
    12421 cggtgtttgc agaggtctgc accggtaaac ggatcgaccg tcctgataac aggcgcttct
    12481 ttcaggttgt gaagcgttgg gcgctgtcta cgacagcata gagcaagggg gagactacgc
    12541 ctgtgccgct ggcgcggaaa ggagaaactg tttttacctg caaaaggatt ttttaatcac
    12601 ttgttgttat aaaaaatgag ggcgctgtat gtgacagcgc cctcgttcag gttacataaa
    12661 aaatatcagc tcagaatagc ttacagtcga gccagcacct cattcacgac agcatcgggt
    12721 atacgttcca gccgcttacc gctccgagct cccatatcaa tggttctggg ctggtcgcag
    12781 cgaataacgc ccgttgtttt tgttcccgcc ccgtccagtg aaacggtaaa accggcttcg
    12841 cgggcaaagt ttccgccgct ggtgacaggg acaacaacag gcaaccgggt gaacgtgtta
    12901 aacgatgcct tcgatacgat gagtaccgga cgttttccac tttgttcatg gccagtggtg
    12961 ggatccagtg aaacgagcca gatttctcct ctgtccatct acaggatctc cttgcccacc
    13021 gcaggcgcat caatccattc ccgaccctct tcgcttattt cagcgtgcgg atcgcactgc
    13081 gccaacagtt cttcgagcga atagcgggga cgtttttgag gttcaataat caggcagcca
    13141 ttatcaatgg tcatgcccac ttcgctgtcc gtcgacagcc ctagcgtttt cagcactgcg
    13201 gggggaaccg ccagcatgac ggatccgcca acctttttca ggcgagtggt atacatagag
    13261 cacctccgga tattatattt taatataaca tccatgatag acgatgcaca actattcatc
    13321 agattcattg tggtttcctt agtgtaataa agcgtgaaga aagccttaat ccatcggaag
    13381 acagatccgg cagttttcaa aggcgacatt gccggtaaaa gggcaaccgt cctgatacat
    13441 caggcgcttc tttcaggtca tgaagcattt cgtacaggcg ggagcttagt gcgtaacaaa
    13501 gaaggccgat aataggaaac tgcttttgca gttcaaagga ttatgacggt tcggataaac
    13561 aaaatgcggc gcagcttccg tatccgggct gcgccgcagg aacatcagcg gtagcgcgaa
    13621 ggccagattt gctcaggctt gatgcctaac gcttcggcaa ttaacctttc gcctttgggc
    13681 cagcggcggg taagcgcatt agccagcgtg gaggacgcca gacctgcctg acgcgaaagc
    13741 gctgacagcg aagtgcctcg ttttttcagc ccggcgataa tgtcggcagg atgccagtcc
    13801 tgcagcatca tgcagccacc tcgtcgataa acttcacgcc atcggcggtg cggagccagc
    13861 gcggggcttt catttcggcg gcaaaatacc agaccagttc acataataag cctgttatcg
    13921 tctgctccac ttttggctgc atcccctgcc cgataagaag ttgcgccagc gtcagtgagt
    13981 aatcacaaag taattctgcg tcggggacaa atcggggcat atcggcaggt agagcatcaa
    14041 cggtcaggct gtcagtcaga tgagacggga tggggtcgtt cagcgtgggt tgcagcaaag
    14101 tgagacaggc ggcaagtcgc ccgcaaagcg ccagccgcag caccggatct tcgctttcga
    14161 tcagggtttc ggcgacgttt tcgcagtggt ctgccagtac ggtaaaatct gtggcagcac
    14221 tgaagggaac agcaagtaac gggtgtgtct gagtggggat cgtagccata aaggcagcct
    14281 ccagtatgtg gtttgaaaac caccaccaga ggttccaatc tcatgggtgg tggactgaac
    14341 gaggttggaa ctaccggcca tactggaacc ggcgcacctt acggtgcccc cgcccagccc
    14401 accataattt tggtgtagct aagcgcgcga cataaaaaaa gacgcagacg cgtcacgtgt
    14461 cgccagtatg gttatatttc ggggttccaa tcccggcacc tgattttgca ggtgcgggta
    14521 aaagataccg ctttcggttg aaaacggcaa ggggaagtcg tggaatagag atcgcagatt
    14581 gttataaata tacacaatag ttaatgttaa caaagacggc acatatatta actttactct
    14641 caatctgctg aatagtttat cgcatgtaat tctcgggtca tatagtggat accctccttc
    14701 ttttctttca tcccctcttg cctcaccata aaagcaaacg aaaaaattgc tgaacgagtt
    14761 atctgaagta aatgctcaaa atggtgaata aaatcacttt ctttgataaa aagaatatct
    14821 tctattagtt gataagattc gtaaatgtcc tcaggtttat cacttttatg tacaactaaa
    14881 aacttatgct ctaaaccatt gcgccattct ttatagaaag ccaactcccc acccttatct
    14941 ttattcaggt cggtagcaat actgtacagc gccagaagcc ctggtgattt taccttttca
    15001 aataattgtc ttctgttatc aacatctaac tgccagaaat tctgaaaata gatattcttc
    15061 tgaggtgtac ctttttttgt tgttgggtaa agattataaa gttcacatat ggctaccgca
    15121 attttatcaa gaataccaaa gcaaagacga aatgccgtcc tgattttttc aatttctatt
    15181 cctaaaactt catcattata caattccaga aaatggcttt cgtacttaat gtaatccgta
    15241 ttttgtggat gaagataatc aaaataaaga tggcgtgcga gtgaaaactc cgatttaagg
    15301 cgatttaata ccatctccat gggaatgata aaatcacctg ttacaccgcc tcgcgaagca
    15361 atcacaagat catctgtagc actccctaca catgggcaat acaggccatg ttctgataaa
    15421 gtcagatgat ttctaagaca aaactgtcga tatgggctta acacttcgaa ctcttgttgt
    15481 gtaagtgctt catcgtgttc gtcagtttca tcatcaacag catattctgc gattttctcg
    15541 ttagtctgcg ctatgcgttc taaatagaac gattcccatt gtggtgggat attttttgat
    15601 acagacgctt taatatatcc tgctctcact tcttttagta acttgattga aaatgaagat
    15661 gaaataagat tgaggaccat aagtgacatc gaacggttga cccaagcctg tggtatatct
    15721 aatcccttaa gattggtaag atcataatat ctcaatgact cactgagccg aaattgactt
    15781 cttaatgaat tagccaaatt aacagatagt tcagcctgaa attcctcctg ctcctcagca
    15841 gatagcttaa aagaacgcca gtagatattt tttaaggaaa ccaattcttc tatcgatttg
    15901 aatgtatggt cattggggtt agtaatgctc accagattag atagcgcatt accatagtta
    15961 taatagtaaa ggcactctcc taacactgat ataaatgctt ctttgttatc ttccatcagg
    16021 ttgcaaccaa gctctgacgc atttttatta ttttgcatct gcccgatgtc aacaagagca
    16081 cccgcaatgt tcgccaatac tataaagtag ttatcgttcc tgattgttat tctttccagt
    16141 ttgttcagga acgcaactga ctgttgataa gattcgtcaa atcttccaat atcaaccagt
    16201 tggctaatga aattactttc atcgacaagt tcttttgtta tcaaaataaa ttttccttat
    16261 acagaggatt tcggtgcttc aacaggaggt atgccctcac aggagacatc tataactatc
    16321 gctgataagc ggtctctttt atcaaccggc acagtttcat taagcatatt ttccattgca
    16381 ctggcgtttt caggttgaga tgtatttttc gccaaatgag gcagggaatt cggtcgagca
    16441 gaaccaaacc aaaaaaccag atagataccg tgaccagcag catcggggtt tttagtataa
    16501 agcttgtcca attgcccatt aagtgcagtc cagacatcac gatgataatc acgctttatc
    16561 tcgataggta tttttatctg cggtagtgaa acaatgatat ctgccctttt atcagaaacc
    16621 atgtgtcctt ctggttcaca gcttattttt tatggattaa gccggacacg gagtaaatcc
    16681 agaaaaatat tacggcaact ctcttcaggt ttaggcgtta gggtacggcc atagctgtct
    16741 tcattccaga attgcttata aatatcggta ttttcattgg caattcggtt agaaatatcc
    16801 cgtatatgat caagcaataa tgagtaaaga tccattatgt tggcaggcgt tttattcatc
    16861 aatgtactta cagtctgcct ccagtcggca tgatggaact gtgattctct ataacgaatt
    16921 ttctgattgc tttgggcatg tgagagatga tcccgataac tttcacattc aggcagcagt
    16981 aataagcgct caagtacttc actagcatcg
//
LOCUS       DAJTSL010000083        16752 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTSL010000083 DAJTSL010000000
VERSION     DAJTSL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.083"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..2020
                     /gene="fhuE"
                     /locus_tag="OO433_002443"
     CDS             <1..2020
                     /gene="fhuE"
                     /locus_tag="OO433_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCR6050882.1"
                     /translation="NDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEV
                     MENTLEISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVE
                     VVRGATGLMTGTGNPSAAIDMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTED
                     GKIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTW
                     GGLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEV
                     EFDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYE
                     LFGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDT
                     THMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWS
                     TYASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSGWMNSRLTTTLAIFRIEQDNVA
                     QSTGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAV
                     NPNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDL
                     FTRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(2080..3513)
                     /gene="ptsG"
                     /locus_tag="OO433_002444"
     CDS             complement(2080..3513)
                     /gene="ptsG"
                     /locus_tag="OO433_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6050883.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMHDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(3808..4605)
                     /locus_tag="OO433_002445"
     CDS             complement(3808..4605)
                     /locus_tag="OO433_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6050884.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(4616..5620)
                     /gene="holB"
                     /locus_tag="OO433_002446"
     CDS             complement(4616..5620)
                     /gene="holB"
                     /locus_tag="OO433_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6050885.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(5617..6258)
                     /locus_tag="OO433_002447"
     CDS             complement(5617..6258)
                     /locus_tag="OO433_002447"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6050886.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(6248..7270)
                     /gene="yceG"
                     /locus_tag="OO433_002448"
     CDS             complement(6248..7270)
                     /gene="yceG"
                     /locus_tag="OO433_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6050887.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(7273..8082)
                     /gene="pabC"
                     /locus_tag="OO433_002449"
     CDS             complement(7273..8082)
                     /gene="pabC"
                     /locus_tag="OO433_002449"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6050888.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(8202..9443)
                     /gene="fabF"
                     /locus_tag="OO433_002450"
     CDS             complement(8202..9443)
                     /gene="fabF"
                     /locus_tag="OO433_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6050889.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNLDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(9531..9767)
                     /gene="acpP"
                     /locus_tag="OO433_002451"
     CDS             complement(9531..9767)
                     /gene="acpP"
                     /locus_tag="OO433_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6050890.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(9978..10712)
                     /gene="fabG"
                     /locus_tag="OO433_002452"
     CDS             complement(9978..10712)
                     /gene="fabG"
                     /locus_tag="OO433_002452"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6050891.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(10725..11654)
                     /gene="fabD"
                     /locus_tag="OO433_002453"
     CDS             complement(10725..11654)
                     /gene="fabD"
                     /locus_tag="OO433_002453"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6050892.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(11670..12623)
                     /gene="fabH"
                     /locus_tag="OO433_002454"
     CDS             complement(11670..12623)
                     /gene="fabH"
                     /locus_tag="OO433_002454"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6050893.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVANQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(12691..13761)
                     /gene="plsX"
                     /locus_tag="OO433_002455"
     CDS             complement(12691..13761)
                     /gene="plsX"
                     /locus_tag="OO433_002455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6050894.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLRPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(13842..14015)
                     /gene="rpmF"
                     /locus_tag="OO433_002456"
     CDS             complement(13842..14015)
                     /gene="rpmF"
                     /locus_tag="OO433_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6050895.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(14067..14588)
                     /gene="yceD"
                     /locus_tag="OO433_002457"
     CDS             complement(14067..14588)
                     /gene="yceD"
                     /locus_tag="OO433_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6050896.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            14787..15371
                     /gene="yceF"
                     /locus_tag="OO433_002458"
     CDS             14787..15371
                     /gene="yceF"
                     /locus_tag="OO433_002458"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6050897.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLCEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(15483..16442)
                     /gene="rluC"
                     /locus_tag="OO433_002459"
     CDS             complement(15483..16442)
                     /gene="rluC"
                     /locus_tag="OO433_002459"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6050898.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
BASE COUNT         4162 a         4546 c         4047 g         3997 t
ORIGIN      
        1 aaatgattac agcgtaacgt ctacctctgc gggtaccaaa atgcagatga ctcaacgtga
       61 tattcctcag tcggtcacta ttgttagcca gcagcggatg gaagatcagc agttacaaac
      121 gctgggcgaa gtgatggaaa acacgctgga gatcagcaaa agtcaggcgg attccgatcg
      181 tgctctttat tattcccgcg gattccagat cgataactat atggttgatg gtatccccac
      241 ctattttgaa tcgcgctgga atctgggcga tgcactttct gatatggcac tgtttgaacg
      301 cgtagaagta gtgcgtggcg cgacaggact catgaccggg acgggtaatc catctgcggc
      361 aattgatatg gttcgaaaac acgcgaccag tcgtgaattt aaaggcgatg tctcggcgga
      421 atacggtagc tggaacaaag aacggtatgt ggcggattta caaagcccac tcaccgaaga
      481 cggtaaaatc cgcgcgcgaa ttgtcggcgg ctaccagaat aacgactcat ggctggaccg
      541 ctacaacagt gaaaagacct tcttctccgg cattgtcgat gctgatttag gcgatcttac
      601 gacgctttca gccggttacg aatatcagcg cattgatgtt aatagcccta cctggggcgg
      661 tttaccgcgc tggaatactg atggcagcag caacagttac gatcgtgcac gcagtaccgc
      721 acctgactgg gcgtacaacg ataaagagat caacaaggtc tttatgaccc tgaagcagcg
      781 gtttgctgat acctggcaag cgacactgaa tgccacccac tctgaagtcg aatttgacag
      841 caaaatgatg tatgtcgatg cctatgtaaa caaagcggat ggtatgctgg ttgggccata
      901 cagtaattat ggacctggct ttgattatgt cggcggcacc ggttggaaca gtggcaaacg
      961 taaagttgat gcgctggatt tgttcgctga cggtagttat gaattgtttg gtcgtcagca
     1021 caatctaatg tttggtggca gttacagcaa acaaaacaat cgttacttca gttcatgggc
     1081 caacatcttc ccggatgaaa ttggcagttt ctacaacttt aatggcaatt tcccacaaac
     1141 cgactggtca ccacagagcc tggcacagga cgataccaca catatgaaat cgttatatgc
     1201 tgccactcga gtcacccttg ccgatccgct gcatctgatc ctcggcgcac gttataccaa
     1261 ctggcgggtt gatacgctga cttacagcat ggagaaaaac cacaccacgc cttacgctgg
     1321 tctggtgttt gacatcaatg acaactggtc gacctacgcc agctatacct ctattttcca
     1381 gccgcaaaat gatcgtgaca gttcaggcaa atatctggct ccaatcaccg gtaacaacta
     1441 cgagctgggt ctgaaatcgg gctggatgaa tagccgtctg accaccacgt tagccatctt
     1501 ccgtattgag caggataatg tcgctcagtc caccggtaca cctatccccg gcagcaacgg
     1561 cgaaaccgcc tataaagcgg tggatgggac agtcagtaaa ggggtggaat ttgaactcaa
     1621 cggcgcaatt accgacaact ggcagctgac atttggcgca acgcgctata ttgcagagga
     1681 taacgaagga aacgccgtta atcctaatct gccacgcacc acggttaaaa tgttcaccag
     1741 ctatcggttg cctgtcatgc cagagttgac agtcggcggt ggtgtgaact ggcaaaatcg
     1801 cgtgtatacc gacaccgtga caccgtatgg caccttccgc gccgagcaag gtagctacgc
     1861 gctggtggat ctcttcaccc gctaccaggt gacgaaaaac ttctcgttac aggggaacgt
     1921 caataacctg ttcgacaaaa cctacgatac caacatggaa ggttctatcg tctacggcgc
     1981 accgcgtaat ttcagcatta ccggcacgta tcaattctga ataacacctg taaaaaaggc
     2041 agccatctgg ctgccttagt ctccccaacg tcttacggat tagtggttac ggatgtactc
     2101 atccatctcg gttttcaggt tatcggattt agtaccgaaa atcgcctgaa caccagaacc
     2161 cgcaaccact acgcccgctg cgcccagttt cttcaggcca gcctgatcca ctttagacac
     2221 atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg aggttagtaa tgttttcttt
     2281 accaccaaat gcagcaacca gagccggtgc catttcgctg gtacctgttg cttttgcatc
     2341 ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt gctttaatca gcacgcggaa
     2401 gatggtgtag taaacaatcg cataaccgat accgacgatc gggaacagcc acagtttgct
     2461 gctgttacca gacagaacga taaagtcgat cagaccgtgc gagaacgacg taccgtcatg
     2521 catccccaga agaatacaga ttgggaatgc caggcctgcc agaatcgcgt ggatgatgta
     2581 caggatcggc gcaacgaaca tgaaggagaa ctcgatcggc tcggtgatac cggtcaggaa
     2641 cgaggtcagc gccgcggaga tcataatacc gcccactttc gcgcggtttt ctggtttagc
     2701 agagtgccag atagcaattg cggcagctgg cagaccgtac attttgaaca ggaagccacc
     2761 agacagttta cccgcagtcg ggtcacccgc catataacgc ggaatgtcgc cgtggaaaac
     2821 ctgacctgct gcgttggtgt attcaccaat ctgcatctgg aaaggtacgt tccagatgtg
     2881 gtgcagacca aacggtacca ggcaacgttc gatgaaaccg taaatgccaa acgcaactac
     2941 cgggttctgg taagcagccc actgagagaa ggtctggatt gcagaaccaa tcggcggcca
     3001 aatgaaggac agcacaacgc cagtaaagat ggcagccagg ccagaaatga tcggcacaaa
     3061 gcgtttaccg gcaaagaagc caagatactc aggcagctta atacggtaga aacggttaaa
     3121 catgtacgct gcgatcgcac cggagataat ccctccgagt acgccagtat ccgctaggtg
     3181 tttagaggcg atttcttcag caggtaaatg cagtaccagt ggcgcaacca cggccatggt
     3241 tttaaccatg atgccatagg caacaactgc ggccagcgcg gatacgccat cgttattggt
     3301 aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg tttgcaaaga cggaaccgcc
     3361 tgcttctgcc ataacatgcg atacaacggc gggcagccag ctgaaattcg cggaaccgac
     3421 gcccagcaga atacctgcga taggtagtac ggataccggc agcatcagcg atttaccgac
     3481 cttttgcagg ttagcaaatg cattcttaaa cataattgag agtgctcctg agtatgggtg
     3541 ctttttttac gttctcacgc atggcaaggg gggagagcct cgccgtgtac agggcatcta
     3601 agcgcccttt atttattaca cagagtaaaa taattcagtg ccaatttgtt tgacggctat
     3661 cacgtttcaa cttcagatca ttggagaagt tgcacatttt gacaaaacat atgtcacaat
     3721 atttcaaacg cggttattca ccgcccaatt ttcggcggtg aactttactc gttttagcca
     3781 ttaattacga gctttaaaaa aactcattca acggatggat tgaaggcggg aagcgtcgat
     3841 gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc agttcttcaa cggcaacacc
     3901 tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc gcaggttgat tctcttttcc
     3961 tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc accagtaatc gatccagggg
     4021 gacataacgc gcagcatcgc gcagttgctc cgcattacgg aaggtcacaa tgccggaaaa
     4081 ggagatgtaa aatccgagat ccagtaattt acccgccgtt tctctgtcct ctgtaaaaca
     4141 gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc agaattgcca gcgtatcggc
     4201 acgggcgtca cgggtatgga cgataaccgg cttgttcagt tcacgaccaa tctggatatg
     4261 atggatgaag gactcttgct gacgtacttt agtttccggt gtgtaataat aatccagccc
     4321 ggtttcaccc agcgctacaa caccctcttc tgccgccaga cggcgtaaat cttctacatc
     4381 gtagggatca ttctggttga gtggatgcac gccacaagaa aatacgacgt tgtcacgttc
     4441 gcctaccaga tcccgcatat gtaaataacc cggtaatgtt gtggcgactg ccagacaaaa
     4501 tttcacatcg cgtgcggcgg ctttcgccag aacgtcatcc acgtccttat gcaaagattc
     4561 ataatccaga ccatcgagat ggcagtgtga gtcgactaaa aacatgatgt ctctcttaaa
     4621 gatgaggaac cggtagcaca acgcccggtt gcaggtaatg ctcaatacgc agtaaaagat
     4681 cggtgatgag aagctcgcgg ttgatgcctg tgacagacat taactgttca cgaatgtggc
     4741 aaacatcccc cagtatagcc tgcaggcgcg agggagaaag atggtttgcc agttcggcga
     4801 ccaggcccgg cacatcaaca ttggtcacct gtgcagcgcc atgatggcgt tttagcgcat
     4861 ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc ttgttcatga ttaagggccg
     4921 ccagcagcga ataccagtcg cccgatggta cgctatatgc caacgcctga cacaatgttt
     4981 cacgagcctg ccagttatct ccctgaaaca acgccagtgc cgcgctaggc gaaccggcac
     5041 ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt cacttcgcgt gaaagccagg
     5101 tcacggcgta ctgttccggc ggcggcgcaa ggtaatgtaa ccgacaacga ctacgtaatg
     5161 ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa ccaggtttct gctggtggct
     5221 cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt cagtaaggca gcatcggtta
     5281 cccagacaac tttcgcacca cctaggcgtg cgtgctcatt cagcttttcg gtgacctcac
     5341 gtaccgcatc aatgcccagc gtatttttcc ctttttcggg agccagggtg tagtaatcgg
     5401 gatgcgtgcc agcctgcatc aactgacatc cacgacagtg accgcaactt ttgtggccct
     5461 gcggttgttg gcagagtaaa taacggctca gggcgtagat taaagcatca tcgcccatgc
     5521 ccggtaacgc ctgaatgagt agcgcatggt gacctcttcc ggcctgatag ctggctacca
     5581 gtttttcgaa atcaggtcgt aaccatggat accatctcat gcgtccaact ccttcaccca
     5641 gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc agcggctggg tggcatcaat
     5701 ggtatgaata cttttatctt gtgcagccag ttccagatag cgggcgcggg tgcgattaaa
     5761 gaaatcgaaa gactcttgct caatacgatc cagctcgccg cgcgcacgcg cgcgttttaa
     5821 gccaacttcc ggggtaacat cgagatagag cgttaagtca gggcgaaaat ccccgagaac
     5881 agcatcacgc agtgttgcca gcatatgttg gtcaatacca cgtcctccgc cctgatacgc
     5941 ctgagtggag agatcgtggc gatcgccaat cacccaggtg ccgttagcca gagctggttt
     6001 gatgaccgtt tctaccagtt gaacgcgcgc ggcataaaac atcagaactt cggctttatc
     6061 ggtaatgact tcatcgccta ccgatttgat atccagcacc aggcttctta acttttcggc
     6121 aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg cggataccca gttgctcaag
     6181 cgtctcaacc accacattac gcgcggtagt tttgcctgcg ccttccagcc cctcaatgac
     6241 gatatactta ctgcgcattt ttttccttaa gcactttcag ataatcctgc acagacttgt
     6301 tatggctggc aagattggta ttaaacgtgt gaccaccttt accatcggcc acaaaataga
     6361 gatacggcgt ttttgccgga tgcgcagcag ccttcagcga atccgccccc ggcgtagcta
     6421 tcgcacctgg cggcaaaccg gtaatggtat aggtgttata cgctgtcggc gtttccaggt
     6481 ctgcacgaga aagtttgcca ttataacgct ctcccatccc gtaaatcacg gttgggtcgg
     6541 tctgcaggcg cataccaatg cgtaaacggt tgataaatac cgaggcaacc tgatcgcgtt
     6601 cactggcaac ggcggtttct ttttcgataa ttgatgccat cgtcaccagc tggtttttat
     6661 ctttataagg caggccgtcc gcacgccctt cccaggcgct atcgaccgct ttcaccattt
     6721 tcttgtgcgc tcgcttgagt aacgcgacat cggtggtatt ggcggtatac atccaggtgt
     6781 ctggccagaa ccaaccttca atccactccg ggttttccag ttcaagtgcc tgcgctacgg
     6841 tggcgtactt atcgtcactc agcgtgtgct tgatatacgg agcctcacgc aattgcttga
     6901 ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg gaactgtgct tctttaccgc
     6961 tttccagcaa tttcagcatc tcgcgcacgg tcatctgcgg tgtaaagcgg taagtcccgg
     7021 ctttaaagtg agaaagatcc ggttcgatac gcagcaacca ttgaaaaacc cgtgggcgat
     7081 tgatgatctt atcggcataa agctgttcac caagcgccag acgtccggtc cctggcttca
     7141 gggtaaatat cgtctcttct ttgataagca atttgctgtc ggcaagatgg cgaaccttcc
     7201 agacgcccac accagcggcg atacccagta ccaccagcaa taacaagata atcaataaca
     7261 cttttttcat gactaattcg ggcgctcaca aagtggggct aaatattcat ataacgttgc
     7321 tgacgaaaag gagacatcgc cacaggcacg tacgggcatc actggcatta acgcattaca
     7381 aataaccatc tcatctgcct gcaacgcctc ttccagagag gcttgcactt cgacaagctg
     7441 ataagaggat tgtgccagca aacggataca gaattgtcgc ataatgccgt taacacctgc
     7501 ctgatccagt cgcggcgtat aaactacatt gcccttccgc cagaacaaat tagccgcaca
     7561 gcattccgta acccaccctt cgctgtcaag gaccagcgcc tcatcagcgt ttgtctgctc
     7621 aagatgagag cggatcaata cttgctcaag ccgattaaga tgtttaatac ctgcaagatg
     7681 gggattgcgc cccagccgca ccgggcttag cgccaacgtc atcccttcgt tacgcaaacg
     7741 gtcgtaatgt gcaggataaa ccgtaacgga gagaatccgc gttgccggtc cactgttcaa
     7801 tgtgctgtac cctcgcccgc cactaccgcg actgatcacg actttcagca caccattttg
     7861 ctgttctgct gccagcgttt tcatctcttg ttcaagctga ggccagaagt cacagaaaat
     7921 catcaaccgc tgacaagcat cctgtagtcg ctggatatgc gccgataaca aactgacttt
     7981 accgtcgata actctggcgg tggtaaaaca accatcacca aactgcgttg cccgatcgct
     8041 tactgccagc gattcctgct tatgaccgtt aattaagaac ataccggctc cttatcagag
     8101 aattgttagt gtggcagcat gctcactacg gaacaagtcg gaataaaagc taagaaaaaa
     8161 ggcccgcaag cggacctttt atatgggtgg gaaatgacaa cttagatctt tttaaagatc
     8221 aaagaaccat tagtgccacc gaagccgaag gagttacaca gcgtgtattc cattccgctg
     8281 acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac cttcatccag gttatccagg
     8341 ttgatggttg gcggaacagc ctgatcgcgc agcgccagga tggagtagat agactctact
     8401 gcccccgccg cacctaacag gtgaccggtc atagatttcg tggagcttac caacacacgg
     8461 cttgcagctt caccgaagat ggctttcacc gcctgcgctt cagctttatc gccagccggc
     8521 gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg cttcaatgcc tgcatcacgc
     8581 agagcatttg ccattgccag agctgcgcct gcgccatttt ctggcggtga cgtcatatga
     8641 taagcatcgc tgctcatacc aaagccgacg agttcagcgt aaattttcgc accgcgtttt
     8701 ttcgcgtgtt cgtactcttc tagtaccagc ataccggcac catcgcccag tacgaagcca
     8761 tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt tatcattgcg ggtagataat
     8821 gcacgtgccg cgccaaaacc accaacgccc agcggcgtac tggctttctc tgcgccacct
     8881 gcaaccatca cgtcagcatc gccatacgcg ataatacgcg cagcatgacc aatgttgtgc
     8941 acgccggaag tacaggcggt cgcgatagag atgcttgggc cacgcaggcc atacatgata
     9001 gtcagatgac ctgccaccat gttcacaatc gttgacggaa cgaagaatgg gctgatctta
     9061 cgtggaccac cgttcatcag agatgtgtgg ttttcttcga tcagtccgag gccgccaatc
     9121 ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt ccgttatttc aaggccagaa
     9181 tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa tgaaggcatc catcttgcgc
     9241 tgttctttgc gcgagataat gtcctcacag ttaaaatcct ttactaagcc agcaaatttc
     9301 gttgcatagg cgctagtatc gaaatggtcg attaggctga tgccactctg accggcaagc
     9361 agagctttcc aggtagactc tacggtattg ccgacaggag acaacatgcc cagtccggtc
     9421 acaactacac gacgcttaga cacgtttgtc ctccagggag ggaaaaaatg attctagtgg
     9481 gacaaaaaga taaaactcag gcggtcgaac gaccgcctgg agatgttcac ttacgcctgg
     9541 tggccgttga tgtaatcaat ggcagcctga acggtggtga ttttctcagc ttcttcgtcc
     9601 ggaatctcag tatcaaactc ttcttccaga gccattacca gctcaacggt gtcaagagaa
     9661 tccgcgccca ggtcttcaac gaaagaagca ttgttggtaa cttcttcctg cttaacgccc
     9721 agctgttcgc cgataatttt cttaacgcgt tcttcgatag tgctcatact cttaaatttc
     9781 ctatcaaaac tcgctttcgc gatggttttc gtagtgtata aaatgttgaa aaatttgcaa
     9841 ctaaatcccg gcaggtctta ccacgatttt acgttatttt gaggccttac cccccaataa
     9901 cgcaaatcat tttgcactaa ttattactca taaccacgca gactgcgcgc aacatgagca
     9961 ttttgtgcaa atcgcggtca gaccatgtac atcccgccgt tcacatgcaa agtttcaccc
    10021 gtgatgtaag ctgcttcgtc ggatgccagg aatgcaaccg cgttggcgat ttcctgtgcg
    10081 ccgccgaggc gacccgcagg aacctgcgcc aggatacccg cacgctggtc atcgctcagc
    10141 gcacgtgtca tgtccgtttc aataaagccc ggagcaacaa cgtttacagt aataccgcgt
    10201 gacgcaactt cgcgcgccag tgatttactg aagccgatca agcccgcttt cgccgcagcg
    10261 tagttggcct gaccgccatt tcccatggta ccaaccacag aaccgatagt gataatacga
    10321 ccatgacgct ttttcatcat agcgcgcatt accgcttttg acagacggaa aacagatgaa
    10381 aggttggttt cgataatatc gttccactct tcatctttca ttcgcattaa caggttatca
    10441 cgagtgatac cggcattatt gaccaggata tccacttcac caaattctgc gcgaattttt
    10501 tccagaacag attcgataga tgccgggtcg gtcacattca acatcagacc tttgccgtta
    10561 gcacctaaat aatcactgat cgcctgagcg ccattttcac tggtcgcagt gccaataact
    10621 ttcgcgccac gggctgcgag cgtttcagca attgcgcggc caattccgcg gcttgcaccg
    10681 gttaccagtg cgatttttcc ttcaaaattc atgattttcc tcttttaaag ctcgagcgcc
    10741 gctgccatcg ctgaaggttc gttcagcgcc gaggcggtca gggtgtcgac aatgcgtttc
    10801 gtcaggccag ttagcacttt gcccgggccg acttcataga gatgttctac gccttgcgct
    10861 gccatgtact caacagactt cgtccactga accgggttat acaactgacg taccagtgcg
    10921 tcacgaatgg catcaccatt ggtttcgcat ttcacatcaa cgttattcac aacaggaact
    10981 gttggtgcgt taaaggtgat tttcgctaat tctactgcca gtttgtcggc tgctggtttc
    11041 atcagcgcac agtgagacgg tacgctcact ggtaacggca gcgcgcgttt tgcgcccgca
    11101 gctttacagg cagcgccagc acgctcaacc gcctctttat gaccggcaat aaccacctgt
    11161 cccggagagt taaagtttac cggagaaacg acctgacctt ctgcagcttc ttcacacgct
    11221 ttcgcaatag acgcatcatc cagaccgatg attgccgcca tagcgcccgt gccttctggt
    11281 acggcttctt gcatgaactt gccgcgcatc tcaaccagac gcaccgcatc agcgaaatca
    11341 atcacgccag cgcaaaccaa cgcggagtat tcccccaggc tgtgaccggc catcattgcc
    11401 ggtgctttac cgccctgctg ctgccatacg cgatacagcg caacagatgc agtcaacagc
    11461 gctggctggg tttgccaggt tttattcagt tcttcagctg gcccctgctg ggtcagcgcc
    11521 cacaggtcgt agcccagcgc cgcagaagct tcagcaaacg tttcttcgac aattggatag
    11581 ctcgccgcca tatcagccag cattccaacg gtttgagaac cctgtccagg gaacacaaat
    11641 gcaaattgcg tcatgtttta atccttatcc tagaaacgaa ccagcgcgga gccccaggtg
    11701 aatccaccgc caaaggcttc aagcagaacc aactgccccg gcttaatgcg cccgtcgcgt
    11761 acagcttcat ccagcgcgca cgggacagag gccgcagagg tattaccgtg gcgatccaac
    11821 gtcaccacga cattgtccat agacataccg agttttttcg ccgttgcact gataatacgc
    11881 aggttagcct gatgcggaac cagccagtcc agttgagaac ggtcaagatt attcgccgcc
    11941 agcgtctcat caacgatgtg cgccagttcc gttaccgcaa ccttgaagac ttcgttgccc
    12001 gccatcgtca gatgaattga attctctgga ttcacgcggt cggcgtttgg cagcgtcagc
    12061 aattcaccat aactaccgtc ggcatgcaga tgggtggaga tgattcccgg ctcttcagag
    12121 gcagccagca ccgcagcgcc cgcgccatcg ccaaaaataa taatagtccc acgatctgtt
    12181 ggatcgcagg tgcgcgccag tacatcggaa ccgacgacca gagcatactt caccgcccca
    12241 gatttcacgt attgattggc tacgcttaat gcataggtga aacctgcgca ggctgctgca
    12301 acgtcaaatg ccgggcaacc tttaatgccc aacatgcttt gaatctgaca agctgcgctc
    12361 gggaaagcgt gcgtagcaga agtcgttgcc acaacgatca ggccaatctg gtctttctca
    12421 atgcccgcca tctcaattgc gcgtgtcgcc gcttcaaagc ccatggttga aacggtttcg
    12481 tttggcgcgg caatgtggcg ttcgcggata ccggtacgag tgacaatcca ctcgtcagag
    12541 gtgtccacca ttttttccaa atcggcgttt gtccgcactt gttcgggcag atagctgcca
    12601 gtaccaataa tcttcgtata catgtacgct cagtcacttt tcggttatat accgtcactt
    12661 gcaaactgcg agttcgctgg cagcgtcctg ctaccgcaga gttccgcttt tgccaccgtc
    12721 cagcagctca aaaccagctg ggtatacaga ttccaggcga gcggcaattc gctgaggaac
    12781 ttgtcgctgc accgcctgca ctgcctgttc aatcgcgacc gcaaaagctc gctgattggc
    12841 tgcaccatga ctttttatca ccgtgccgcg caatcctaac agacaggcgc cgttatactg
    12901 gtcggggttg aggtgactga atcgcctcgt caggctcttt tgtagccaac gctttaataa
    12961 cagtagccac cacgaccgtt ttttcccttc accctgagat ttcagcagag aaaggaacat
    13021 cctgacaaca ccttccatcg tctttaacgt gacatttcct gtaaagccgt cacaaaccag
    13081 cacatctgtc ttgccagtta acaactcatt ggcttcaaga tagccgatat aattgataga
    13141 agggattgtt ttaagcaccg ctgaggcatc ccgaatactg tcgagaccct ttacttcttc
    13201 ttcaccaata ttgagcaacg ccacgcgagg attgggaatt tccaccacct cttcagccag
    13261 aactgagccc ataatggcaa attgcaccaa cattgtgctg tcacaatcga cgttggcccc
    13321 taaatcgagg accaccgttt tgcccttttg ctgatgtggt aataccgtca ccagcgccgg
    13381 acgctcaatc ccctccaggg gcctgagtaa taattttgcc agccccatca gtgccccggt
    13441 attaccggca ctgacacagg cttgcgctcg accttctttc accagctcca gggccatacg
    13501 cattgaactc ccacgactag cgcggatagc ttgcgaaggc cgggcatcac tggcgataac
    13561 tgactgcgca ggaataatct gcagacgcga acgttgttca aagtcagctt tagcaagtaa
    13621 tggcgtgatg gcgtcgggat tgccgactaa aagaagagtg agttgcgaat tagaattcag
    13681 tgcctgcaat gctgcaggca ctgtcacgga agggccaaaa tcccctccca tgacatctaa
    13741 cgccagggtt agacgtgtca aggtatcgtc gccgcccggt ttggtctttc cccagttgcc
    13801 tggggaaaat cctcactaag cttcatcacg cagacgcgtg attacttagc gatgaccttg
    13861 cggccgcggt agtaaccgtc ggcagtgata tggtgacgca ggtgtttttc accagaagtt
    13921 ttgtctacag acaggctggt gactgcggtc agcgcgtcat gggaacgacg catgccacgt
    13981 ttggaacggg ttggtttatt ctgttgtacg gccatggacc ttactcctca attacggttt
    14041 atccccgatc caacggggag caccaattac ttacgcttta agctggctaa tacggcaaat
    14101 gggtttggct tttgcacttc ttcaggcagt tcaccaaaga ccatgtccgc ttcggacact
    14161 tcacagtgtt cagaatcatg caccggaact accggcaagg cgaggatgat ttcatcttca
    14221 accattgcaa gcagatcgat ttcaccgaat tcgttaacct caatcggttc atacgcttcc
    14281 ggcagtgctt cagcctgttc gtctgaacgc acaggactaa aacaatacgt tgtgtagacc
    14341 tgatgagtaa acggcttccc gcaacgctga cactcgagcg ttaccgtcac cttcgcatcg
    14401 ccgtttaaca ctgcgagacg ttggttatcg atagcgaacg acatggagca ttccacatca
    14461 ctgtccacac tgactacgga ttcggcgacg cgctcaacct gatcaggggt atagataccc
    14521 tggtaatcaa ggcgtttttg agccgtacga accggatcga gagtcagggg taattttacc
    14581 ttttgcatag ggcgcgcata ttaactttgt aacgtcatag agtcaaagaa aaaggcagcc
    14641 tgtggttgcc ttttgccaat aattcgcaca cattgcgggt tactgcttta ttttcgttca
    14701 gtgctgataa cgaacatcaa cctgcaaaac gaagcgcata gtttaaaatg gctatcatca
    14761 atacgctata tctgatggaa aaaaatatgc ctaaacttat tttagcctcc acctcgccat
    14821 ggcgtcgcgc tcttctggaa aaactgcaaa tctcttttga atgtgcagca ccagaggtcg
    14881 acgaaacccc acgcagcgac gaatcaccgc gacagttggt gcttcgactg gcacaagaaa
    14941 aagcgcaatc tctggcgtca cgttatccgg atcatttaat tattggttca gaccaggtat
    15001 gtgttcttga tggcgaaatc accggtaaac cgttaacgga agaaaatgcc cgtctgcaat
    15061 tacgcaaagc cagcggcaat atcgtcacct tctatactgg actggcgttg tttaattcgg
    15121 cgaatgggca tctacaaaca gaagtggagc cttttgacgt ccatttccgt cacctgtgcg
    15181 aggcggagat tgataattac gtgcgtaagg agcatcccct gcactgcgcg ggtagcttta
    15241 agagtgaagg atttggcatt acgctgtttg agcgtttaga gggacgtgac cctaacacgc
    15301 tggttggttt accgctaatc gcgctgtgtc agatgttacg tcgggaaggg aaaaacccgc
    15361 tgatgggata gttgtttgcg ccggatgcgg ttacgtcgca tccggcaatc ggtgcaggcg
    15421 gcctgatgcg gcgtaaacgc cttatcaggc caaccgccca ttatcaggct tatatgcttg
    15481 tattagcgcg cgttacgcag cttttgcaaa caacgcttca aaccttcatc catcggcgct
    15541 tcgatacgca tcacctcacc ggtccccgga tgggtaaact tcaacgctgc ggcgtgcagg
    15601 aacagacgat ttaatcccgt gcctgcttca gtgagctgtc tgtcaaattc acggtcaccg
    15661 tagcgatcgt caaaggcaat cggatgaccc gcatactgtg tatgcacacg gatctgatga
    15721 gtacgtccgg ttaccgggct acaacgcacc agggtggcaa atgcatagcg ttcttccact
    15781 ttaaagcgtg tttccgacgg tttgccttcc tgactgacac gcacgatacg ttcgccgctt
    15841 tgcagaatat ttttcaataa cggcgcttga acgctcttca catgcgactg ccactgactg
    15901 cgcaccagcg ccaggtaatc tttttgcatt cctttttcac gtaattgctc atgcagagaa
    15961 cgcaacgccg agcgtttttt cgctaccagc aacacacctg aagtgtcccg gtcgagacga
    16021 tgaaccagtt caaggaaccg cgcttccggg cgcaacgccc gcaaaccttc gataacgccg
    16081 aagcttaaac cactgccgcc atgtaccgcc gtaccggaag gtttattcag caccaggata
    16141 tgatcatctt catataagat gacgtccgcc agcgccgcca ccttttgcag atgtggcgaa
    16201 accgcctctt cttcccgctc agcaacgcga accggtggga tacgcacctc atcacccgct
    16261 tcgagtttat attcaggctt aatacgtttt ttgttcaccc gcacttcgcc tttacgcaaa
    16321 atacggtaaa tcatactttt tggtacgcct ttcaattggg tacgcaaaaa gttgtcgata
    16381 cgttgccccg cttcgtcagc ggtgatagca acaattttta cggatggagt ctctgttttc
    16441 atggtgggcg attctaatta gccaacagga ttcgcgccac tcatttttct atgcttatat
    16501 ttagttttac accttattac ttcactacgt gatcacttta ttgatggtta ttaaaccaat
    16561 caccagcaag aagtgaaaaa tgtaatgaac gcatcccaca caattaaaca cggagcagta
    16621 tgatggttaa aagggtgaaa ctggcaggtg tggagcctcg cagatttccg gcatcatagt
    16681 gcccaatacg ggatgcataa agctccctca gactggagac tccacaaatg aaatatacac
    16741 cggttggcgt tg
//
LOCUS       DAJTSL010000084        16722 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTSL010000084 DAJTSL010000000
VERSION     DAJTSL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.084"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            125..745
                     /locus_tag="OO433_002460"
     CDS             125..745
                     /locus_tag="OO433_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCR6050899.1"
                     /translation="MKDQDVRFAVHHKLLKESHLDPDCLVVDEFSISLGASRADIAVI
                     NGVIHGYELKSEYDSLERLPLQIKHYSSVMDKVTLVVAEKHLEGALKLIPGWWGVKTV
                     SVGPKGAILIKHMRGEKLNRNHDTLMLAQLLWKDECIDVLERWGYSKGIKSKPRFELW
                     NIIAENIPIANLRLEVRTALKKRVGWKVKAWQAESAPTNKAVSRLT"
     gene            complement(687..1754)
                     /locus_tag="OO433_002461"
     CDS             complement(687..1754)
                     /locus_tag="OO433_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HCR6050900.1"
                     /translation="MSQHQYYPQLKWKPAEYESLMLLDQTTLSGFTPIITIPDIDWDY
                     ENECYKKSLSSYLSDFGINLAASWKANRPVLLDVKYLDKHGSSRHHPLDMCIQDARVN
                     GKEIIPVVSPAYSTNYIHAVQRNLINGLAISITPQTWHQFTSLVNHLNIHPSLIDVII
                     DFGDIQNATDSLKQQALSMVNTLSGQAPWRNLILSSTAYPASQAGIPQHQVHHIPRHE
                     YDLWMYVVQNFSNGRTPSFSDYPTASSTITSVDPRFMSQYVSVRYSNDTSWIFVKGTA
                     VKGNGWGQTKNLCTTLVSSPEYQVFGSKFSWGDDYIYQRSLGANKSGGSKEWRKVAHT
                     HHITLVVRQLYWLAQTQPAKP"
     gene            2167..2712
                     /locus_tag="OO433_002462"
     CDS             2167..2712
                     /locus_tag="OO433_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR6050901.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            2687..4612
                     /locus_tag="OO433_002463"
     CDS             2687..4612
                     /locus_tag="OO433_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6050902.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            4609..4815
                     /locus_tag="OO433_002464"
     CDS             4609..4815
                     /locus_tag="OO433_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCR6050903.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            4812..6413
                     /locus_tag="OO433_002465"
     CDS             4812..6413
                     /locus_tag="OO433_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6050904.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            6394..7713
                     /locus_tag="OO433_002466"
     CDS             6394..7713
                     /locus_tag="OO433_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6050905.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            7723..8055
                     /locus_tag="OO433_002467"
     CDS             7723..8055
                     /locus_tag="OO433_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6050906.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDSAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8111..9136
                     /locus_tag="OO433_002468"
     CDS             8111..9136
                     /locus_tag="OO433_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6050907.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGSTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            9178..9573
                     /locus_tag="OO433_002469"
     CDS             9178..9573
                     /locus_tag="OO433_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6050908.1"
                     /translation="MTKDELIVRLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTPLRPENALTGHENEVVSAQTDTVTDAAALVTVVALVTLHTDALHATRDEP
                     VAFVLPETAFRVSAGVAAEMTERGLARMQ"
     gene            9585..9938
                     /locus_tag="OO433_002470"
     CDS             9585..9938
                     /locus_tag="OO433_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCR6050909.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9950..10528
                     /locus_tag="OO433_002471"
     CDS             9950..10528
                     /locus_tag="OO433_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6050910.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            10525..10920
                     /locus_tag="OO433_002472"
     CDS             10525..10920
                     /locus_tag="OO433_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6050911.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            10928..11668
                     /locus_tag="OO433_002473"
     CDS             10928..11668
                     /locus_tag="OO433_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6050912.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDKDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAVVAEITVTDS"
     gene            11684..12106
                     /locus_tag="OO433_002474"
     CDS             11684..12106
                     /locus_tag="OO433_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6050913.1"
                     /translation="MFLKTESFEYNGVSVTLSELSALQRIEHLALLKRQAEQAGSSLN
                     RQVSVEDLVRTGAFLVAMSLWHSHPQKTKMPSMNEAVKQIEQEVLTTWPTEAIAQAEN
                     VVMRLSGMSEFVVNDAPEQADDAGPAEPVSAGKCSTVS"
     gene            12088..12522
                     /locus_tag="OO433_002475"
     CDS             12088..12522
                     /locus_tag="OO433_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6050914.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSIHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            12515..15076
                     /locus_tag="OO433_002476"
     CDS             12515..15076
                     /locus_tag="OO433_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6050915.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMNRQALAAQKVGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SQLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQCRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            15073..15402
                     /locus_tag="OO433_002477"
     CDS             15073..15402
                     /locus_tag="OO433_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6050916.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            15402..16100
                     /locus_tag="OO433_002478"
     CDS             15402..16100
                     /locus_tag="OO433_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6050917.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYAHFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            16106..>16722
                     /locus_tag="OO433_002479"
     CDS             16106..>16722
                     /locus_tag="OO433_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6050918.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFYREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQL"
BASE COUNT         3943 a         4112 c         5084 g         3583 t
ORIGIN      
        1 tagtcgtatg gctatgaatc ttgttgctgc agataagcat tttgtgattg aagtggtcaa
       61 atctctcaaa tgcagtaaaa tgcagtgcgc tataattcaa taacggaggg agtaaggaga
      121 agtcatgaaa gatcaagatg ttaggttcgc ggtgcatcat aagcttttga aagaatcgca
      181 tttagatcca gactgccttg tggtcgatga attttccata tcccttggcg ccagtagagc
      241 agacattgct gtaataaatg gtgttataca cgggtacgag ctcaaaagtg aatatgactc
      301 tttggagcgt ttgcctcttc aaatcaagca ttattcttct gtaatggaca aggttactct
      361 tgtcgtagct gagaaacatc ttgagggagc attaaagtta atcccaggtt ggtggggcgt
      421 taaaacggtt tctgttgggc caaaaggcgc cattcttata aagcacatgc gtggagaaaa
      481 gcttaatcga aaccatgaca cattgatgct cgctcaattg ctttggaaag atgaatgtat
      541 cgacgtactt gaacgatggg gctattccaa aggaatcaaa agcaagcccc gatttgagtt
      601 atggaatatt attgcggaaa atattccaat agcgaatctc aggcttgaag tcagaacagc
      661 cttaaagaaa cgcgtaggct ggaaagttaa ggcttggcag gctgagtctg cgccaaccaa
      721 taaagctgtc tcacgactaa cgtaatatgg tgcgtatgtg caactttacg ccattcttta
      781 gagccgccag atttgttagc gcctaatgat ctttggtaaa tgtaatcatc cccccaacta
      841 aatttggaac caaagacttg atactctggc gaactaacaa gggtagtaca taagttttta
      901 gtttggcccc atccatttcc tttaactgcg gtacctttta caaagatcca tgaggtatcg
      961 ttcgaatatc tcactgagac atactgagac atgaagcgtg ggtctacgct cgtaatggta
     1021 gagctagcgg tgggataatc actaaaactt ggcgttcttc cattgctaaa attctgtact
     1081 acgtacatcc aaagatcgta ttcatggcgc ggaatatgat gaacttgatg ttgcggtatc
     1141 cctgcctgtg atgctgggta tgcggttgaa gataaaatca agtttctcca cggagcttgg
     1201 cctgataatg tgttgaccat gcttaatgct tgttgtttta aactatcagt tgcgttttga
     1261 atatctccaa aatcaatgat tacatcaatt aaactaggat gaatatttaa gtggttaacc
     1321 agacttgtga attggtgcca tgtctggggg gtgatagata tagcgagccc attgattaag
     1381 ttgcgttgaa cagcatgtat atagtttgtt gaatatgcgg gagaaacaac agggataatt
     1441 tccttaccat ttactctagc atcttggata cacatatcta gaggatgatg gcggctcgaa
     1501 ccatgtttat ctaaatattt aacatccagc aaaacaggac gattggcttt ccaggatgcc
     1561 gcaaggttaa taccgaagtc agataagtaa gaactcaaac tcttcttgta gcattcgttt
     1621 tcataatccc agtctatgtc tggaatggta atgatcggag taaaaccaga gagcgtagtt
     1681 tgatctaaaa gcatcagaga ttcatattca gcaggcttcc atttcagctg tggataatat
     1741 tgatgttgac tcattaaaac tccttaaaat ctttacttat cctaagtata gtgttatggc
     1801 gtaacgccaa ttgtttttat acacttgaaa acattgggaa aagttgagct atatctaaat
     1861 ttacaaaatt ggatgttata gaaatcgagg tttcaactag gcatggaact gcaaaatttt
     1921 taatgcctgc acgcgaaagt cgttggcggg tcctttccgg tgatccagac cgttacgggg
     1981 cggcgacctc gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc
     2041 ttcttcgtcg taacttaatg tttttattta aaataccccc tgaaaagaaa ggaaacgaca
     2101 ggtgctgaaa acgaactttt gggcctttgt cgtttccttt ctctgttttt ggccgtggaa
     2161 tgaacaatgg aagtcaacaa aaagcagctg gctgacattt tcggtgcgag tatccgtacc
     2221 attcagaact ggcaggaaca gggaatgccc gttctgcgag gcggtggcaa gggtaatgag
     2281 gtgctttatg actctgccgc cgttataaaa tggtatgccg aaagggatgc tgaaattgag
     2341 aacgaaaagc tgcgccggga ggttgaagaa ctgcgccagg ccagcgaggc agatctccag
     2401 ccagggacta ttgagtacga acgccatcga cttacgcgtg cgcaggccga cgcacaggaa
     2461 ctgaagaatg ccagagactc agctgaagtg gtggaaaccg cattctgtac tttcgtgctg
     2521 tcgcggatcg caggtgaaat tgccagtatt ctcgacggga tccccctgtc ggtgcagcgg
     2581 cgttttccgg aactggaaaa ccgacatgtt gatttcctga aacgggatat catcaaagcc
     2641 atgaacaaag cagccgcgct ggatgaactg ataccggggt tgctgagtga atatatcgaa
     2701 cagtcaggtt aacaggctgc ggcattttgt ccgcgccggg cttcgctcac tgttcaggcc
     2761 ggagccacag accgccgttg aatgggcgga tgctaattac tatctcccga aagaatccgc
     2821 ataccaggaa gggcgctggg aaacactgcc ctttcagcgg gccatcatga atgcgatggg
     2881 cagcgactac atccgtgagg tgaatgtggt gaagtctgcc cgtgtcggtt attccaaaat
     2941 gctgctgggt gtttatgcct actttataga gcataagcag cgcaacaccc ttatctggtt
     3001 gccgacggat ggtgatgccg agaactttat gaaaacccac gttgagccga ctattcgtga
     3061 tattccgtcg ctgctggcgc tggccccgtg gtatggcaaa aagcaccggg ataacacgct
     3121 caccatgaag cgtttcacta atgggcgtgg cttctggtgc ctgggcggta aagcggcaaa
     3181 aaactaccgt gaaaagtcgg tggatgtggc gggttatgat gaacttgctg cctttgatga
     3241 ggatattgaa caggaaggct ctccgacgtt ccttggcgac aaacgtattg aaggctcggt
     3301 ctggccaaag tccatccgtg gctccacgcc caaagtgaga ggcacctgcc agattgagcg
     3361 tgcagccagt gaatccccgc attttatgcg ttttcatgtt gcctgcccgc actgcgggga
     3421 ggagcagtat cttaaatttg gcgacaaaga gacgccgttt ggcctcaaat ggacgccgga
     3481 tgacccctcc agcgtgtttt atctctgcga gcataatgcc tgcgtcatcc gccagcagga
     3541 gctggacttt actgatgccc gttatatctg cgaaaagacc gggatctgga cccgtgatgg
     3601 cattctctgg ttttcgtcat ccggtgaaga gattgagcca cctgacagtg tgacctttca
     3661 catctggaca gcgtacagcc cgttcaccac ctgggtgcag attgtcaaag actggatgaa
     3721 aacgaaaggg gatacgggaa aacgtaaaac cttcgtaaac accacgctcg gtgagacgtg
     3781 ggaggcgaaa attggcgaac gtccggatgc tgaagtgatg gcagagcgga aagagcatta
     3841 ttcagcgccc gttcctgacc gtgtggctta cctgaccgcc ggtatcgact cccagctgga
     3901 ccgctacgaa atgcgcgtat ggggatgggg gccgggtgag gaaagctggc tgattgaccg
     3961 gcagattatt atgggccgcc acgacgatga acagacgctg ctgcgtgtgg atgaggccat
     4021 caataaaacc tatacccgcc ggaatggtgc agaaatgtcg atatcccgta tctgctggga
     4081 tactggcggg attgacccga ccattgtgta tgaacgctcg aaaaaacatg ggctgttccg
     4141 ggtgatcccc attaaagggg catccgtcta cggaaagccg gtggccagca tgccacgtaa
     4201 gcgaaacaaa aacggggttt accttaccga aatcggtacg gataccgcga aagagcagat
     4261 ttataaccgc ttcacactga cgccggaagg ggatgaaccg cttcccggtg ccgttcactt
     4321 cccgaataac ccggatattt ttgatctgac cgaagcgcag cagctgactg ctgaagagca
     4381 ggtcgaaaaa tgggtggatg gcaggaaaaa aatactgtgg gacagcaaaa agcgacgcaa
     4441 tgaggcgctc gactgcttcg tttatgcgct ggcggcgctg cgcatcagta tttcccgctg
     4501 gcagctggat ctcagtgcac tgctggcgag cctgcaggaa gaggatggtg cagcaaccaa
     4561 caagaaaaca ctggcagatt acgcccgtgc cttatccgga gaggatgaat gacgcgacag
     4621 gaagaacttg ccgctgcccg tgcggcactg catgacctga tgacaggaaa acgggtggca
     4681 acggtacaga aagacggacg gagagtggag tttacggcca cttccgtgtc tgacctgaaa
     4741 aaatacattg cggagctgga agtgcagacc ggcatgacac agcgacgcag gggacctgca
     4801 ggattttatg tatgaaaacg tccaccattc ccacccttct ggggccggac ggcatgacat
     4861 cgctgcgtga atatgccggt tatcacggcg gtggcagcgg atttggaggg cagttgcggg
     4921 cgtggaaccc accgagtgaa agtgtggatg cagccctgct gcccaacttt acccgtggca
     4981 atgcccgcgc agacgatctg gtacgcaata acggctatgc cgccaacgcc atccagttgc
     5041 atcaggatca tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc tggcgctatc
     5101 tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag
     5161 agtttgccga ggatgactgc tgctgcattg acgttgagcg aaaacgcacg tttaccatga
     5221 tgattcggga aggtgtggcc atgcacgcct ttaacggtga actgttcgtt caggccacct
     5281 gggataccag ttcgtcgcgg cttttccgga cacagttccg gatggtcagc ccgaagcgca
     5341 tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg cagattaatg
     5401 acagcggtgc ggcgctggga tattacgtca gcgaggacgg gtatcctggc tggatgccgc
     5461 agaaatggac atggataccc cgtgagttac ccggcgggcg cgcctcgttc attcacgttt
     5521 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     5581 agatgaagat gctcgacacg ctgcagaaca cgcagctgca gagcgccatt gtgaaggcga
     5641 tgtatgccgc caccattgag agtgagctgg atacgcagtc agcgatggat tttattctgg
     5701 gcgcgaacag tcaggagcag cgggaaaggc tgaccggctg gattggtgaa attgccgcgt
     5761 attacgccgc agcgccggtc cggctgggag gcgcaaaagt accgcacctg atgccgggtg
     5821 actcactgaa cctgcagacg gctcaggata cggataacgg ctactccgtg tttgagcagt
     5881 cactgctgcg gtatatcgct gccgggctgg gtgtctcgta tgagcagctt tcccggaatt
     5941 acgcccagat gagttactcc acggcacggg ccagtgcgaa cgagtcgtgg gcgtacttta
     6001 tggggcggcg aaaattcgtc gcatcccgtc aggcgagcca gatgtttctg tgctggctgg
     6061 aagaggccat cgttcgccgc gtggtgacgt taccttcaaa agcgcgcttc agttttcagg
     6121 aagcccgcag tgcctggggg aactgcgact ggataggctc cggtcgtatg gccatcgatg
     6181 gtctgaaaga agttcaggaa gcggtgatgc tgatagaagc cggactgagc acctacgaga
     6241 aagagtgcgc gaaacgcggt gacgactatc aggaaatttt tgcccagcag gtccgtgaaa
     6301 cgatggagcg ccgtgcagcc ggtcttaaac cgcccgcctg ggcggctgca gcatttgaat
     6361 ccgggctgcg acaatcaaca gaggaggaga agagtgacag cagagctgcg taatctcccg
     6421 catattgcca gcatggcctt taatgagccg ctgatgcttg aacccgccta tgcgcgggtt
     6481 ttcttttgtg cgcttgcagg ccagcttggg atcagccgcc tgacggatgc ggtgtccggc
     6541 gacagcctga ctgcccagga ggcactcgcg acgctggcat tatccggtga tgatgacgga
     6601 ccacgacagg cccgcagtta tcaggtcatg aacggcatcg ccgtgctgcc ggtgtccggc
     6661 acgctggtca gccggacgcg ggcgctgcag ccgtactcgg ggatgaccgg ttacaacggc
     6721 attatcgccc gtctgcaaca ggctgccagc gatccgatgg tggacggcat tctgctcgat
     6781 atggacacgc ccggcgggat ggtggcgggg gcatttgact gcgctgacat catcgcccgt
     6841 gtgcgtgaca taaaaccggt atgggcgctt gccaacgaca tgaactgcag tgcaggtcag
     6901 ttgcttgcca gtgccgcctc ccggcgtctg gtcacgcaga ccgcccggac aggctccatc
     6961 ggcgtcatga tggctcacag taattacggt gctgcgctgg agaaacaggg tgtggaaatc
     7021 acgctgattt acagcggcag ccataaggtg gatggcaacc cctacagcca tcttccggat
     7081 gacgtccggg agacactgca gtcccggatg gacgcaaccc gccagatgtt tgcgcagaag
     7141 gtgtcggcat ataccggcct gtccgtgcag gctgtgctgg ataccgaggc tgcagtgtac
     7201 agcggtcagg aggccattga tgccggactg gctgatgaac ttgttaacag caccgatgcg
     7261 atcaccgtca tgcgtgatgc actggatgca cgtaaatccc gtctctcagg agggcgaatg
     7321 accaaagaga ctcaatcaac aactgtttca gccactgctt cgcaggctga cgttactgac
     7381 gtggtgccag cgacggaggg cgagaacgcc agcgcggcgc agccggacgt gaacgcgcag
     7441 atcaccgcag cggttgcggc agaaaacagc cgcattatgg ggatcctcaa ctgtgaggag
     7501 gctcacggac gcgaagaaca ggcacgcgtg ctggcagaaa cccccggtat gaccgtgaaa
     7561 acggcccgcc gcattctggc cgcagcacca cagagtgcac aggcgcgcag tgacactgcg
     7621 ctggatcgtc tgatgcaggg ggcaccggca ccgctggctg caggtaaccc ggcatctgat
     7681 gccgttaacg atttgctgaa cacaccagtg taagggatgt ttatgacgag caaagaaacc
     7741 tttacccatt accagccgca gggcaacagt gacccggctc ataccgcaac cgcgcccggc
     7801 ggattgagtg cgaaagcgcc tgcaatgacc ccgctgatgc tggacacctc cagccgtaag
     7861 ctggttgcgt gggatggcac caccgacagt gctgccgttg gcattcttgc ggttgctgct
     7921 gaccagacca gcaccacgct gacgttctac aagtccggca cgttccgtta tgaggatgtg
     7981 ctctggccgg aggctgccag cgacgagacg aaaaaacgga ccgcgtttgc cggaacggca
     8041 atcagcatcg tttaacttta cccttcatca ctaaaggccg cctgtgcggc tttttttacg
     8101 ggattttttt atgtcgatgt acacaaccgc ccaactgctg gcggcaaatg agcagaaatt
     8161 taagtttgat ccgctgtttc tgcgtctctt tttccgtgag agctatccct tcaccacgga
     8221 gaaagtctat ctctcacaaa ttccgggact ggtaaacatg gcgctgtacg tttcgccgat
     8281 tgtttccggt gaggttatcc gttcccgtgg cggctccacc tctgaattta cgccgggata
     8341 tgtcaagccg aagcatgaag tgaatccgca gatgaccctg cgtcgcctgc cggatgaaga
     8401 tccgcagaat ctggcggacc cggcttaccg ccgccgtcgc atcatcatgc agaacatgcg
     8461 tgacgaagag ctggccattg ctcaggtcga agagatgcag gccgtttctg ccgtgctcaa
     8521 gggcaaatac accatgaccg gtgaagcctt cgatccggtt gaggtggata tgggccgcag
     8581 tgcggcgaac aacatcacgc agtccggcag cacggagtgg agcaagcgtg acaagtccac
     8641 gtatgacccg accgacgata tcgaagccta cgcgctgaac gccagcggcg tggtgaatat
     8701 catcgtgttt gatccgaaag gctgggcgct gttccgttcc ttcaaagccg tcagggagaa
     8761 gctggatacc cgtcgcggct ctcattccga gctggagaca gcggtgaaag acctgggtaa
     8821 agcggtgtcc tataagggga tgtatggcga tgtggccatc gtcgtgtatt ccggacagta
     8881 cgtggaaaac ggcgtcaaaa agaacttcct gccggacaac acgatggtac tggggaacac
     8941 tcaggcacgc ggtctgcgca cctatggctg cattcaggat gcggacgcac agcgcgaagg
     9001 cattaacgcc tctgcccgtt acccgaaaaa ctgggtgacc accggcgatc cggcgcgtga
     9061 gttcaccatg attcagtcag caccgctgat gctgctggct gaccctgatg agttcgtgtc
     9121 cgtacaactg gcgtaatcgt ggcccttcgg ggccattttc tctctgtgga ggagtccatg
     9181 acgaaagatg aactgattgt ccgtctccgg tcgctgggtg agcaactgaa ccgtgatgtc
     9241 agcctgacgg ggacgaaaga agaactggcg ctccgtgtgg cagagctgga agaggagctt
     9301 gatgacacgg atgacgctgc cggtcaggat acccctctca gaccggaaaa tgcgctgacc
     9361 ggacatgaaa atgaggtggt atcagcacag acggataccg tgactgatgc ggctgctctg
     9421 gtcacggttg tggcactggt gacgctgcat accgatgcac ttcacgccac gcgggatgaa
     9481 cctgtggcat ttgtgctgcc ggaaacggcg ttccgtgtct ctgccggtgt ggcagctgaa
     9541 atgacagagc gcggcctggc cagaatgcaa taacgggagg cgctgtggct gatttcgata
     9601 acctgttcga tgctgccatt gcccgcgccg atgaaacgat acgcgggtac atgggaacgt
     9661 cagccaccat gacatccggt gagcagtccg gcgcagtaat acgtggtgtt tttgatgacc
     9721 ctgaaaatat cagctatgcc ggacagggcg tacgcgttga aggctccagc ccgtccctgt
     9781 ttgtccggac tgatgaggtg cggcagctgc ggcgcggcga cacgctgacc atcggtgagg
     9841 aaaacttctg gatagaccgg atttcgccgg atgatggcgg aagctgtcat ctctggcttg
     9901 ggcggggcgt accgcctgcc gttaaccgtc gccgctgaaa gggggatgta tggccataaa
     9961 aggtcttgag caggccgttg aaaacctcag ccgtatcagt aaaacggcgg tgcccggtgc
    10021 cgccgcaatg gccattaacc gcgttgcttc atccgcgata tcgcagtcgg cgtcacaggt
    10081 tgctcgtgag acaaaggtac gccggaaact ggtaaaggaa agggccaggc tgaaaagggc
    10141 cacggtcaaa aatccgcagg ccagaatcaa agttaaccgg ggggatttgc ccgtaatcaa
    10201 gctgggtaat gcgcgggttg tcctttcgcg ccgcaggcgt cgtaaaaagg ggcagcgttc
    10261 atccctgaaa ggtggcggca gcgtgcttgt ggtgggaaac cgtcgtattc ccggcgcgtt
    10321 tattcagcaa ctgaaaaatg gccgctggca tgtcatgcag cgtgtggccg ggaaaaaccg
    10381 ttaccccatt gatgtggtga aaatcccgat ggcggtgccg ctgaccacgg cgtttaaaca
    10441 gaatattgaa cggatacggc gtgaacgtct tccgaaagag ctgggctatg cgctgcagca
    10501 tcaactgaga atggtaataa agcgatgaaa catactgaac tccgtgcagc cgtactggat
    10561 gcactggaga agcatgacac cggggcgacg ctttttgatg gtcgccccgc tgtttttgat
    10621 gaggaagatt ttccggcaat tgccgtttat ctcaccggcg ctgaatacac gggcgaagag
    10681 ctggacagcg atacctggca ggcggagctg catattgaag ttttcctgcc tgctcaggtg
    10741 ccggattcag agctggatgc gtggatggag tcccggattt atccggtgat gagcgatatc
    10801 ccggcactgt cagatttgat caccagtatg gtggccagtg gctatgacta ccggcgcgac
    10861 gatgatgcgg gcctgtggag ttcagccgat ctgacttatg tcattaccta tgaaatgtga
    10921 ggacgctatg cctgtaccaa atcctacaat gccggtgaaa ggtgccggga ccaccctgtg
    10981 ggtttataag gggagcggtg acccttatgc gaacccgctt tcagacgttg actggtcgcg
    11041 tctggcaaaa gttaaagacc tgacgcccgg cgaactgacc gctgagtcct atgacgacag
    11101 ctatctcgat gataaagatg cagactggac tgcgaccggg caggggcaga aatctgccgg
    11161 agataccagc ttcacgctgg cgtggatgcc cggagagcag gggcagcagg cgctgctggc
    11221 gtggtttaat gaaggtgata cccgtgccta taaaatccgc ttcccgaacg gcacggtcga
    11281 tgtgttccgt ggctgggtca gcagtatcgg taaggcggtg acggcgaagg aagtgattac
    11341 ccgcacggtg aaggtcacca atgtgggacg tccgtcgatg gcagaagatc gcagcacggt
    11401 aacagcggca accggcatga ccgtgacgcc tgccagcact tcggtggtga aagggcagag
    11461 caccacgctg accgtggcat tccagccgga aggcgcaacc gacaagagct tccgtgcggt
    11521 gtctgcggat aaaacaaaag ccaccgtgtc ggtcagtggt atgaccatca ccgtgaaagg
    11581 tgttgctgca ggcaaggtca acattccggt tgtatccggt aatggtgaac ttgctgtggt
    11641 tgcagaaatc accgtcaccg acagttaatc cggagagtca gcgatgttcc tgaaaaccga
    11701 atcatttgaa tataacggtg tgagcgtcac gctttctgaa ctgtcagccc tgcagcgaat
    11761 tgagcatctc gccctgctga aacgacaggc agaacaggcg ggatccagtc tcaatcgaca
    11821 ggtgagcgtg gaagatctcg tcagaaccgg tgcttttctg gtggcgatgt ccctgtggca
    11881 tagccatccg cagaagacaa agatgccgtc catgaatgaa gccgttaaac aaattgagca
    11941 ggaagtgctt accacctggc ccacagaggc aattgctcag gctgaaaatg tggtaatgcg
    12001 tctgtccggt atgtctgagt ttgttgtgaa tgatgcacct gaacaggcag atgacgccgg
    12061 gccagcagag cctgtttctg cgggaaagtg ttcgacggtg agctgagttt tgccctgaaa
    12121 ctggcgcgtg agatggggcg acccgactgg cgcgccatgc ttgccgggat gtcatccacg
    12181 gagtatgccg actggcaccg cttttacagt atccattatt ttcatgatgt tctgctggat
    12241 atgcactttt ccgggctgac gtacaccgta ctcagcctgt ttttcagcga tccggatatg
    12301 catccgctgg atttcagtct gctgaaccgg cgcgaggctg acgaagagcc tgaagatgat
    12361 gtgctgatgc agaaagcggc agggcttgcc ggaggcgtcc gctttggccc ggacgggaat
    12421 gaagttatcc ccgcttcccc ggatgtggcg gacatgacgg aggatgacgt aatgctgatg
    12481 acagtatcag aagggatcgc aggaggagtc cggtatggct gaaccggtag gcgatctggt
    12541 cgttgatttg agtctggatg cggccagatt tgacgagcag atggccagag tcaggcgtca
    12601 tttttccggt acggaaagtg atgcgaaaaa aacagcggca gtcgttgaac agtcgatgaa
    12661 ccggcaggcg ctggctgcac agaaagtggg aatttccgtc gggcagtata aagccgccat
    12721 gcgtatgctg cctgcgcagt tcactgacgt ggccacgcag cttgcaggcg ggcaaagtcc
    12781 gtggctgatc ctgctgcaac aggggggtca ggtgaaggac tccttcggcg ggatgatccc
    12841 catgttccgg gggcttgccg gtgcgatcac cctgccgatg gtgggggcca cctcgctggc
    12901 ggtggcgacc ggagcgctgg cgtatgcctg gtatcagggc aactcaaccc tgtccgattt
    12961 caacaaaacg ctggtccttt ccggcaatca ggcgggactg acggcagatc gtatgctggt
    13021 cctgtccaga gccgggcagg cggcagggct gacgtttaac cagaccagcg agtcactcag
    13081 cgcactggtt aaggcggggg taagcggtga ggctcagatt gcgtccatca gccagagtgt
    13141 ggcgcgtttc tcctctgcat ccggcgtgga ggtggacaag gtcgctgaag ccttcgggaa
    13201 gctgaccaca gacccgacgt cggggctgac ggcgatggca cgccagttcc ataacgtgac
    13261 ggcggagcag attgcgtatg ttgctcagtt gcagcgttcc ggcgatgaag ccggggcatt
    13321 gcaggcggcg aacgaggccg caacgaaagg gtttgatgac cagacccgac gcctgaaaga
    13381 gaacatgggc acgctggaaa cctgggcaga caggacagca cgggcattca aatccatgtg
    13441 ggatgcggtg ctggatattg gtcgtcctga taccgctcag gagatgctga ttaaggcaga
    13501 ggctgcgttt aagaaagcag acgacatctg gaatctgcgc aaggatgatt attttgttaa
    13561 cgatgaagcg cgggcgcgtt actgggatga tcgtgaaaag gcccgtcttg cgcttgaagc
    13621 cgcccgaaag aaggctgagc agcagactca acaggacaaa aatgcgcagc agcagagcga
    13681 tactgaagcg tcacagctga aatataccga agaggcgcag aaggcttacg aacggctgca
    13741 gacgccgctg gagaaatata ccgcccgtca ggaagaactg aacaaggcac tgaaagacgg
    13801 gaaaatcctg caggcggatt acaacacgct gatggcggcg gcgaaaaagg attatgaagc
    13861 gacgctgaaa aagccgaaac agtccggcgt gaaggtgtct gcgggcgatc gtcaggaaga
    13921 cagtgctcat gctgccctgc tgacgcttca ggcagaactc cggacgctgg agaagcatgc
    13981 cggagcaaat gagaaaatca gccagcagcg ccgggatttg tggaaggcgg agagtcagtt
    14041 cgcggtactg gaggaggcgg cgcaatgtcg ccagctgtct gcacaggaga aatccctgct
    14101 ggcgcataaa gatgagacgc tggagtacaa acgccagctg gctgcacttg gcgacaaggt
    14161 tacgtatcag gagcgcctga acgcgctggc gcagcaggcg gataaattcg cacagcagca
    14221 acgggcaaaa cgggccgcca ttgatgctaa aagccggggg ctgactgacc ggcaggcaga
    14281 acgggaagcc acggaacagc gcctgaagga acagtatggc gataatccgc tggcgctgaa
    14341 taacgtcatg tcagagcaga aaaagacttg ggcggctgaa gaccagcttc gcgggaactg
    14401 gatggcaggc ctgaagtccg gctggagtga gtgggaagag agcgccacgg acagtatgtc
    14461 gcaggtaaaa agtgcagcca cgcagacctt tgatggtatt gcacagaata tggcggcgat
    14521 gctgaccggc agtgagcaga actggcgcag cttcacccgt tccgtgctgt ccatgatgac
    14581 agaaattctg cttaagcagg caatggtggg gattgtcggg agtatcggca gcgccattgg
    14641 cggggctgtt ggtggcggcg catccgcgtc aggcggtaca gccattcagg ccgctgcggc
    14701 gaaattccat tttgcaaccg gaggatttac gggaaccggc ggcaaatatg agccagcggg
    14761 gattgttcac cgtggtgagt ttgtcttcac gaaggaggca accagccgga ttggcgtggg
    14821 gaatctttac cggctgatgc gcggctatgc caccggcggt tatgtcggta caccgggcag
    14881 catggcagac agccggtcgc aggcgtccgg gacgtttgag cagaataacc atgtggtgat
    14941 taacaacgac ggcacgaacg ggcagatagg tccggctgct ctgaaggcgg tgtatgacat
    15001 ggcccgcaag ggtgcccgtg atgaaattca gacacagatg cgtgatggtg gcctgttctc
    15061 cggaggtgga cgatgaagac cttccgctgg aaagtgaaac ccggtatgga tgtggcttcg
    15121 gtcccttctg taagaaaggt gcgctttggt gatggctatt ctcagcgagc gcctgccggg
    15181 ctgaatgcca acctgaaaac gtacagcgtg acgctttctg tcccccgtga ggaggccacg
    15241 gtactggagt cgtttctgga agagcacggg ggctggaaat cctttctgtg gacgccgcct
    15301 tatgagtggc ggcagataaa ggtgacctgc gcaaaatggt cgtcgcgggt cagtatgttg
    15361 cgtgttgagt tcagcgcaga gtttgaacag gtggtgaact gatgcaggat atccggcagg
    15421 aaacactgaa tgaatgcacc cgtgcggagc agtcggccag cgtggtgctc tgggaaatcg
    15481 acctgacaga ggtcggtgga gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg
    15541 agccggtcac ctggcagggg cgacagtatc agccgtatcc cattcagggg agcggttttg
    15601 aactgaatgg caaaggcacc agtacgcgcc ccacgctgac ggtttctaac ctgtacggta
    15661 tggtcaccgg gatggcggaa gatatgcaga gtctggtcgg cggaacggtg gtccggcgta
    15721 aggtttacgc ccattttctg gatgcggtga acttcgtcaa cggaaacagt tacgccgatc
    15781 cggagcagga ggtgatcagc cgctggcgca ttgagcagtg cagcgaactg agcgcggtga
    15841 gtgcctcctt tgtactgtcc acgccgacgg aaacggatgg cgctgttttt ccgggacgta
    15901 tcatgctggc caacacctgc acctggacct atcgcggtga cgagtgcggt tatagcggtc
    15961 cggctgtcgc ggatgaatat gaccagccaa cgtccgatat cacgaaggat aaatgcagca
    16021 aatgcctgag cggttgtaag ttccgcaata acgtcggcaa ctttggcggc ttcctttcca
    16081 ttaacaaact ttcgcagtaa atcccatgac acagacagaa tcagcgattc tggcgcacgc
    16141 ccggcgatgt gcgccagcgg agtcgtgcgg cttcgtggta agcacgccgg agggggaaag
    16201 atattttccc tgcgtgaata tctccggtga gccggaggcg tatttccgta tgtcgccgga
    16261 agactggctg caggcagaaa tgcagggtga gattgtggcg ctggtccaca gccaccccgg
    16321 tggtctgccc tggctgagtg aggccgaccg gcggctgcag gtgcagagtg atttgccgtg
    16381 gtggctggtc tgccggggga cgattcataa gttccgctgt gtgccgcatc tcaccgggcg
    16441 gcgctttgag cacggtgtga cggactgtta cacactgttc cgggatgctt atcatctggc
    16501 ggggattgag atgccggact tttatcgtga ggatgactgg tggcgtaacg gccagaatct
    16561 ctatctggat aatctggagg cgacggggct gtatcaggtg ccgttgtcag cggcacagcc
    16621 gggcgatgtg ctgctgtgct gttttggttc atcagtgccg aatcacgccg caatttactg
    16681 cggcgacggc gagctgctgc accatattcc tgaacaactg ag
//
LOCUS       DAJTSL010000085        16609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTSL010000085 DAJTSL010000000
VERSION     DAJTSL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.085"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(28..2217)
                     /locus_tag="OO433_002480"
     CDS             complement(28..2217)
                     /locus_tag="OO433_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCR6050919.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(2426..2501)
                     /locus_tag="OO433_002481"
     tRNA            complement(2426..2501)
                     /locus_tag="OO433_002481"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2466..2468),aa:Ala,seq:ggc)
     gene            complement(2541..2616)
                     /locus_tag="OO433_002482"
     tRNA            complement(2541..2616)
                     /locus_tag="OO433_002482"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2581..2583),aa:Ala,seq:ggc)
     gene            2837..3196
                     /locus_tag="OO433_002483"
     CDS             2837..3196
                     /locus_tag="OO433_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6050920.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            3198..3590
                     /locus_tag="OO433_002484"
     CDS             3198..3590
                     /locus_tag="OO433_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6050921.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGVRAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3641..5056)
                     /gene="gltX"
                     /locus_tag="OO433_002485"
     CDS             complement(3641..5056)
                     /gene="gltX"
                     /locus_tag="OO433_002485"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6050922.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            5316..5391
                     /locus_tag="OO433_002486"
     tRNA            5316..5391
                     /locus_tag="OO433_002486"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5349..5351,aa:Val,seq:tac)
     gene            5436..5511
                     /locus_tag="OO433_002487"
     tRNA            5436..5511
                     /locus_tag="OO433_002487"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5469..5471,aa:Val,seq:tac)
     gene            5558..5633
                     /locus_tag="OO433_002488"
     tRNA            5558..5633
                     /locus_tag="OO433_002488"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5591..5593,aa:Val,seq:tac)
     gene            5638..5713
                     /locus_tag="OO433_002489"
     tRNA            5638..5713
                     /locus_tag="OO433_002489"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5671..5673,aa:Lys,seq:ttt)
     gene            5833..6174
                     /locus_tag="OO433_002490"
     CDS             5833..6174
                     /locus_tag="OO433_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6050923.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLDVSLLNP
                     VEKTTHIDIYI"
     gene            complement(6165..7091)
                     /locus_tag="OO433_002491"
     CDS             complement(6165..7091)
                     /locus_tag="OO433_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6050924.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            7181..8179
                     /locus_tag="OO433_002492"
     CDS             7181..8179
                     /locus_tag="OO433_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6050925.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCTLPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(8176..8394)
                     /locus_tag="OO433_002493"
     CDS             complement(8176..8394)
                     /locus_tag="OO433_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6050926.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(8396..10411)
                     /gene="ligA"
                     /locus_tag="OO433_002494"
     CDS             complement(8396..10411)
                     /gene="ligA"
                     /locus_tag="OO433_002494"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6050927.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIVGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(10482..11468)
                     /gene="zipA"
                     /locus_tag="OO433_002495"
     CDS             complement(10482..11468)
                     /gene="zipA"
                     /locus_tag="OO433_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6050928.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            11698..12459
                     /gene="cysZ"
                     /locus_tag="OO433_002496"
     CDS             11698..12459
                     /gene="cysZ"
                     /locus_tag="OO433_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6050929.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            12644..13615
                     /gene="cysK"
                     /locus_tag="OO433_002497"
     CDS             12644..13615
                     /gene="cysK"
                     /locus_tag="OO433_002497"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6050930.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            13999..14256
                     /gene="ptsH"
                     /locus_tag="OO433_002498"
     CDS             13999..14256
                     /gene="ptsH"
                     /locus_tag="OO433_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6050931.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            14301..16028
                     /gene="ptsI"
                     /locus_tag="OO433_002499"
     CDS             14301..16028
                     /gene="ptsI"
                     /locus_tag="OO433_002499"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6050932.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            16069..16578
                     /gene="crr"
                     /locus_tag="OO433_002500"
     CDS             16069..16578
                     /gene="crr"
                     /locus_tag="OO433_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6050933.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLPGSVTVG
                     ETPVIRIKK"
BASE COUNT         4197 a         4275 c         4073 g         4064 t
ORIGIN      
        1 gataattccc cgtttttttt acgcctgtta atcagctaat ggctgcgggc gaccaatcaa
       61 atacccttgc agatattgca ccccgagctt atgcaatagc gcctgctgct gttgcgtctc
      121 gacaaactcc gcgaccacac tcaatgactt cgctttcgcc agatcggtaa ttgatctcac
      181 aatcatcgca tccagcgtgt tcgtgacaat atctttcaca aagacgccat caattttgat
      241 gatatcagcc tgcaaacgct ttaaccgttc gtagttggca tagccggtgc caaagtcatc
      301 aatcgcaatc cggaaaccaa acttatgcag ctgctcgatg ttgtacatgc tgctttctgc
      361 gttagaaaac gcctgctcct cggtgatctc aagaatgacc gcctgcgggg agatgtgata
      421 acgcttaaac agacgaataa tccgcccggc aatattcttt tgcagcagcg tgagcggcat
      481 taaattgact gaaaagcgcg gtcctttttt gtcgcaaggg tgtgttgcca gccacttcaa
      541 caaggattcc agcacttgca aatcaaaacg cgcgctaagg ttaaactgag caataagggg
      601 cagaaactta tccggggtca taatgccgcc gtcatatttc agtcgcgcga ggatctcatc
      661 ataaccttca ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat caccctgatc
      721 taacgccgtg cgaatggtat tcagcaatag cacctgttta gtggtctgcc cggaaaccat
      781 ctcctctctg ctatccagcg ccagcacatg atgatgtgcg caggattgct ccgccagcca
      841 gcttaactgc cccaacaagg gttgcagggt ttcctgattt ccatcaaaac gcccccaggc
      901 agcaccatag cccatatcca gcccggtatt gttccagtga atttgccgac tattcaggat
      961 gttaaccata tgctggagtc gcccttccgt ttccggcccg ctcagcacta acagcagttc
     1021 actacccggc aattgataca acttttcgtt ttcctgcatc aacggcagca gcgtacggca
     1081 aattgagcgg atacagtgaa cgcgcatcat taagccgtaa tgacgactca taaactcaag
     1141 attatcaatg cgcaggcagc aaaaactctt gcccgcctct tgctccggcg cttgttccaa
     1201 cgcacggaag ttgggcagga gcgttaacgg atcggtcagc gcctgcaaat gccagcggcg
     1261 attaagccat tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga
     1321 aatcagcacc gcaagaataa atgccagcga atattcggtt tccacccctt gcaaaaagtt
     1381 ctggttgtaa ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata
     1441 gcgaagctta ccgaccccga gggtaaagat gatgaagaaa acgggtacca ggtaaccggc
     1501 aataaagtcg ttttcataag gtgtgcacaa cagaagtaga agcacgctta acgctgccag
     1561 ccagcttaag gtaaatgcgc gtttctcttt gcccaacgac ggcgcgatat ccctgcgcca
     1621 caatatctgc gcaaagtggg gacttacaat catgcgagtg agatagtaga aaagcatgtt
     1681 gtaaatcagc acagcggtga aaaggcttag caaatcaacg accgtgaaaa tggcatccgc
     1741 atcgccgaaa aaggtggata tctttagcgg gaaatcaaag aagctcccca caagatacat
     1801 gctgcatttg atgccgatcg gcgtcaccaa accaagccag aacagacgtt gccagacata
     1861 tcggctggtc aatccataac gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc
     1921 acacaacacg acaaacgtct ggctaccgag catgaccgca gtctgtaaaa aagagagatg
     1981 aaagttccac agattggtgc aaaacatccc gcataaaacg ggaacgacac ctcgccagcc
     2041 aaagataaac aagattgaca gcattacgca cagtggcatc cacgccagaa agatataact
     2101 ggaatggata atggcaagtg gcgaaataaa acgggatagc tgaataagta ccacggtgag
     2161 cgtaaacgct agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag
     2221 tacactactc tcactgatcg gagttttcag gcacacaaat aataagttta ttcgcatcgc
     2281 gaagcaaaca gggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg
     2341 gcactaaccc ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc
     2401 cgctttgcag cgagggttgg aaatttggtg gagctaagcg ggatcgaacc gctgacctct
     2461 tgcatgccat gcaagcgctc tcccagctga gctatagccc cacgatgcgt ttacgtacca
     2521 aatttgctgg gtgcaaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
     2581 gccatgcaag cgctctccca gctgagctat agccccgtca cgtaaagctt gtcgagttga
     2641 cgggcggcat catatgaatt ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt
     2701 caatcgctga aaaatcaggc aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta
     2761 ttcatgtcac ggtttcctgt aaagtggtgt tataaaatga actactaata gacccacata
     2821 cattcaggga attgttatgt tcaaggagag gatgacacca gatgaacttg ccagactgac
     2881 cggctatagc cgccagacca ttaataaatg ggtacgcaag gaaggctgga caacgtcacc
     2941 aaaacctggc gtccagggtg gcaaagcccg actggttcac gtcaatgaac aggttcgtga
     3001 atatattcgc aatgctgaac gtccagaagg tcagggagaa gcgcctgccc tttctggtga
     3061 tgcgccgctt gaagtattgc tggtgacact ggcgaaagag atgacgccag ttgaacaaaa
     3121 acagtttaca tccttgcttc tgcgggaagg gattatcgga ttgttacaac gcttagggat
     3181 tcgcgatagc aaataatatg aaaagattac gcaataaaat gaccaccgaa gaactggctg
     3241 aatgcctcgg tgtggctaaa caaactgtta accgttggat cagagaaaaa ggctggaaaa
     3301 cggaaaaatt tcccggcgtg aaaggcggtc gtgccagact tattctggtc gatacgcaag
     3361 tttgcgagtt tattcagaac acgccagcct tccataacac accaatgttg atggaagctg
     3421 aagagagaat tgctgaatac gctcctggag tacgcgcccc ggcgtatcga caaatcatca
     3481 acgcgataga taatatgaca gacattgagc aggaaaaagt cgcgcaattt ttgtcacgtg
     3541 aaggaattcg caatttcctc gcccgtctcg atattgacga atcagcataa ataaaaaacg
     3601 gcaggatatt atctcctgcc gtttatcttt tatgcgctaa ttactgctgg ttttcgcgtt
     3661 cagcaataaa agccagcgct ttgttgatac gctcaatact gcgggtccta ccaattgcgt
     3721 gaacggtaac atccagtgcc ggagactgcc ccgcaccggt tacggcgaca cgcagcggca
     3781 taccaacttt acccataccc acttccagct catccgccgt cgcctgaata gcgtgatgaa
     3841 cgttttcagc ggtccagtca gtaatcgcgg ccagtttgtc acgaaccact tccagcggct
     3901 gacgcgctac cggacgcaga tgttttttcg cggcgtcggc atcgaactca gcaaaatctt
     3961 cgtagaaata acggcagctc tgtgccatct ctttcagcgt cttgcagcgt tcgcccagca
     4021 gtttcaccag atcagccagc tgcgggccgt tacgggtatc gatattttcc tgctcaatgt
     4081 gccactgtaa gtgagtagca acatactccg gcggcagcgc gttaatgtag tgatggttca
     4141 gccacagcag cttgtcggtg ttgaacgcac tggcagattt gctgacggca ttcagggtga
     4201 agtatttgat catctcttca cgagtgaaga tttcctgatc gccgtgggac cagcccagac
     4261 gcaccagata gttcagcagt gcttctggca aataaccgtc atcacgatac tgcattacgc
     4321 tgaccgcccc gtgacgtttg gacagttttt taccgtcatc gccgttgatc atagaaacgt
     4381 gcgcgtaaac cggcaccggc gctttcaggg ccttaagaat gttgatctgg cgtggcgtgt
     4441 tgttgatatg gtcttcgcca cggataacgt gggtgatttc catatcccag tcatcgacaa
     4501 ccacacagaa gttataggtt ggggaaccat cggtacggcg aataataaga tcgtccagtt
     4561 cctggttgct gaactcgatc ggaccacgga tctgatcgtc aaaaacaaca gaaccttcct
     4621 gcgggttagc aaaacgtaca acgcacggtt catcatcagc gtgatgctca tggctgtggc
     4681 ggcagcgacc gtcataacgc ggcttctcac ctttcgccat ttgctcttcg cgcagcgctt
     4741 ccaggcgctc tttagagcaa tagcatttat aagcagtgcc ctcttccagc atctgatcga
     4801 tcaccgcgtt gtagcgatca aaacgtttgg tctggtagta cggaccttca tcccactcca
     4861 ggctcagcca gttcatgcca tccataatgg cttcgatagc ttccggcgtg gaacgctcaa
     4921 gatcggtgtc ttcaatacgc agcacgaact caccgccgtg gttacgtgca aaaagccagg
     4981 agtaaagggc agtacgcgcg ccgccaacgt gcagatagcc tgttgggctt ggcgcgaagc
     5041 gagttttgat tttcatgaaa tggccttacg tttagaaaga tgccgacaac cggcaaatcc
     5101 tggaaaaatt gagtgggcga tattctatca ctcccgcctg attcctcaat gtagttcggg
     5161 tttctacctt acgcattatt gttttcgttg acaaattgcg caacacggtc tattttgcga
     5221 tcatcttgct taaatttacg acgaacgaac aatttcttta gaaaatgcgt tgactcattt
     5281 tgaactctcc ctataatgcg actccacaca gcggggggtg attagctcag ctgggagagc
     5341 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc aactacttta
     5401 tgtagtctcc gccgtgtagc aagaaattga gaagtgggtg attagctcag ctgggagagc
     5461 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc actttctcac
     5521 cagctaaatt tcttgtaaca atgtgaagta ccgaagtggg tgattagctc agctgggaga
     5581 gcacctccct tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca ccacttcggg
     5641 tcgttagctc agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct
     5701 gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatc tgcgatttgt
     5761 aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc gtacagtgac
     5821 gaggactgaa caatggccac tatctcttca acttcaattc cttccataca aacacagagt
     5881 agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat aagtcagcaa
     5941 atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag tgctgaagac
     6001 aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac acaactggcg
     6061 cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt gtctctggat
     6121 gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat ctgatacaac
     6181 ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac gtcccataac
     6241 gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa ctggcgttca
     6301 acaaccggcg taatgcgctt taccaccagt gggctacctt caggcaatgg tagcgccagt
     6361 gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt cgccggatgt
     6421 ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc gtcaatcagc
     6481 ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa tgcctgccag
     6541 ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa gaaaggctcg
     6601 gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac gatgccaaag
     6661 tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc gtgcaggaca
     6721 aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat aagatgcgcg
     6781 gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc catacgcccg
     6841 gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa agccaactgc
     6901 tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg aacaccagta
     6961 tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc gatacgctct
     7021 cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa ttgttttaaa
     7081 gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat ttctcaaact
     7141 ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt ttcgtatcct
     7201 cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct ttccggccag
     7261 aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc tgctgttctt
     7321 tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc actggcgact
     7381 gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg tactgtttgc
     7441 ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct acttgtgcac
     7501 tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg gtaacgtcgc
     7561 ggcggcggtt tgttctgcgt cagcatccag cctgctggga attttccttt caccattgct
     7621 ggttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg tcggtaaaat
     7681 tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt ggattggtga
     7741 ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca ttctgttggt
     7801 ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag ttggctgggg
     7861 atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga ttgtagttaa
     7921 cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta tcgtcttttg
     7981 tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt tccccacatc
     8041 ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat tgatggtctg
     8101 tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc agcaggaaag
     8161 cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg tcagcccctc
     8221 ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg ggaactcatc
     8281 tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac gccctttgta
     8341 tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt ccatctcagc
     8401 tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt tcctgcgcct
     8461 tcgccagttt agatcctgca gcttcaccca caatcaccag atcggttttc ttcgacacgc
     8521 tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca cgcgacatct
     8581 ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca atctcttccg
     8641 cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg ctgatgacat
     8701 tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca atgccaacat
     8761 caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc gtgccgaaat
     8821 atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt gcatagagga
     8881 agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt tgtgccgatt
     8941 ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac agatctgccg
     9001 gagtgtggac atattctttt tcaaccagct gatcgatgat tttgtcgccc attccgtcaa
     9061 catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc gcaccgcaaa
     9121 tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg tcagaaccac
     9181 ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt tcagaaagca
     9241 cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact ttatcgccaa
     9301 tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta ctcaccagca
     9361 cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca gtacgcccaa
     9421 cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat ttaaacgcta
     9481 ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc agtgagttga
     9541 ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct tccactttgt
     9601 gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg ctgaccggca
     9661 acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc tcgccacctt
     9721 ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta atacgcggat
     9781 caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact ttcccgcccg
     9841 tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac acttcaccac
     9901 gcacttccag acgcgccggg atattctctc catgcagctt cagcggaatg gcgcgaatag
     9961 tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc gcggcactga
    10021 ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc agctcacagc
    10081 accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg aaagcaagaa
    10141 agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg cgtatctggc
    10201 tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc gtaatcagtt
    10261 ctggatgttt agtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat tcagcgtcgg
    10321 gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc gtcgttcgca
    10381 gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa aaacccccga
    10441 caagcggggg ttcgaagaag agttaatttg ccttaagtgt atcaggcgtt ggcgtctttg
    10501 acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat acggcgctga
    10561 tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg cagcatcagc
    10621 ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt gacacccgga
    10681 gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt cgccaggctg
    10741 aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt catatcgcca
    10801 aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag ctcgctaccg
    10861 tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt atccataact
    10921 ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc aggctggaaa
    10981 gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc gacctgtggc
    11041 gaaactggct gctgcaacgg ctgttcaggc tgcggctgat agacaggctg ctgcaccggc
    11101 tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg ctggaccggt
    11161 tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca
    11221 gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca
    11281 acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt
    11341 cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaacca
    11401 tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc
    11461 tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca tttactcact
    11521 aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat
    11581 agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt
    11641 acccggtaaa atacgcagaa ttttcctagg attggtcaaa aggagctcat cctgattatg
    11701 gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag
    11761 ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg
    11821 ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat cccgactctc
    11881 atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc tctggctgtc
    11941 atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg gattgccgct
    12001 ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc tacaccgcca
    12061 gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa
    12121 aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt catccctggt
    12181 attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc
    12241 cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc
    12301 ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct gtttacgatg
    12361 atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg
    12421 gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta
    12481 aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt gcatgtcatt
    12541 atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta
    12601 tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga
    12661 agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg
    12721 acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg
    12781 tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt
    12841 tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta
    12901 caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc
    12961 gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg caatccaaaa
    13021 agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa
    13081 tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg aagataccga
    13141 cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag
    13201 ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg ttgagccaac
    13261 cgattctcca gttatcgccc aggcgctggc aggtgaagag attaaacctg gcccgcataa
    13321 aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc tggtcgataa
    13381 agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa tggaagaaga
    13441 aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga aactacaaga
    13501 agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg gtgagcgtta
    13561 tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac agtaatgcca
    13621 gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa
    13681 ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta
    13741 ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt
    13801 ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac
    13861 acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctattaa aaactggcgc
    13921 taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct
    13981 ataagttggg gaaatacaat gttccagcaa gaagttacca ttacagctcc gaacggtctg
    14041 cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact
    14101 gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc
    14161 ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca aaaagcggtt
    14221 gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca
    14281 gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg
    14341 gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg
    14401 accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc
    14461 tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg
    14521 aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta
    14581 aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg
    14641 ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg
    14701 gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg
    14761 atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag ctgaacctga
    14821 agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg
    14881 cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa
    14941 atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg
    15001 aagttattga taaaatgcgt gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg
    15061 ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa gtatgcgcta
    15121 acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc
    15181 tgtatcgtac tgagttccta ttcatggacc gcgacgcgct gcccactgaa gaagaacagt
    15241 ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca
    15301 tggacatcgg cggcgacaaa gaactgccat acatgaactt cccgaaagaa gagaacccgt
    15361 tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg cgcgatcaac
    15421 tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca
    15481 tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc
    15541 gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg
    15601 ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca
    15661 atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt
    15721 accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg
    15781 ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc
    15841 tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta
    15901 agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg
    15961 ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa
    16021 tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc
    16081 gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat tgagatcatt
    16141 gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa
    16201 aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta
    16261 gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga atctgatagc
    16321 ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc
    16381 aagcgtattg cagaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat
    16441 ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg
    16501 gacgaaatca aagaactgat caaactgccc ggtagcgtaa ccgtggggga aaccccggtt
    16561 atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcg
//
LOCUS       DAJTSL010000086        16569 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTSL010000086 DAJTSL010000000
VERSION     DAJTSL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.086"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            6..1106
                     /gene="arnB"
                     /locus_tag="OO433_002501"
     CDS             6..1106
                     /gene="arnB"
                     /locus_tag="OO433_002501"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6050934.1"
                     /translation="MEELAAVKEVLESGWITTGPKNQALEQAFCQLTGNQHAIAVSSA
                     TAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDVDRDTLMVTPEAIE
                     SAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVGTYYKGRHIGAKGT
                     AIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDRQTWGRAPQAEVLT
                     PGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPFQPLSLPAWPHVHA
                     WHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYRERFPTLSLPNTEW
                     NSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1110..2078
                     /gene="arnC"
                     /locus_tag="OO433_002502"
     CDS             1110..2078
                     /gene="arnC"
                     /locus_tag="OO433_002502"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6050935.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2078..4060
                     /gene="arnA"
                     /locus_tag="OO433_002503"
     CDS             2078..4060
                     /gene="arnA"
                     /locus_tag="OO433_002503"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR6050936.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHATRQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4057..4947
                     /locus_tag="OO433_002504"
     CDS             4057..4947
                     /locus_tag="OO433_002504"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6050937.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKTEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            4947..6599
                     /gene="arnT"
                     /locus_tag="OO433_002505"
     CDS             4947..6599
                     /gene="arnT"
                     /locus_tag="OO433_002505"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6050938.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6596..6931
                     /gene="arnE"
                     /locus_tag="OO433_002506"
     CDS             6596..6931
                     /gene="arnE"
                     /locus_tag="OO433_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6050939.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            6931..7317
                     /gene="arnF"
                     /locus_tag="OO433_002507"
     CDS             6931..7317
                     /gene="arnF"
                     /locus_tag="OO433_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6050940.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7311..7577)
                     /gene="pmrD"
                     /locus_tag="OO433_002508"
     CDS             complement(7311..7577)
                     /gene="pmrD"
                     /locus_tag="OO433_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6050941.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7687..9042)
                     /gene="menE"
                     /locus_tag="OO433_002509"
     CDS             complement(7687..9042)
                     /gene="menE"
                     /locus_tag="OO433_002509"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6050942.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQY
                     ALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9039..10001)
                     /gene="menC"
                     /locus_tag="OO433_002510"
     CDS             complement(9039..10001)
                     /gene="menC"
                     /locus_tag="OO433_002510"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6050943.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELLQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALRLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10001..10858)
                     /gene="menB"
                     /locus_tag="OO433_002511"
     CDS             complement(10001..10858)
                     /gene="menB"
                     /locus_tag="OO433_002511"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6050944.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10873..11631)
                     /gene="menH"
                     /locus_tag="OO433_002512"
     CDS             complement(10873..11631)
                     /gene="menH"
                     /locus_tag="OO433_002512"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6050945.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11628..13298)
                     /gene="menD"
                     /locus_tag="OO433_002513"
     CDS             complement(11628..13298)
                     /gene="menD"
                     /locus_tag="OO433_002513"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6050946.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPSLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13387..14682)
                     /gene="menF"
                     /locus_tag="OO433_002514"
     CDS             complement(13387..14682)
                     /gene="menF"
                     /locus_tag="OO433_002514"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6050947.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14761..15066)
                     /gene="elaB"
                     /locus_tag="OO433_002515"
     CDS             complement(14761..15066)
                     /gene="elaB"
                     /locus_tag="OO433_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6050948.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQVSDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15121..15582)
                     /locus_tag="OO433_002516"
     CDS             complement(15121..15582)
                     /locus_tag="OO433_002516"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6050949.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15647..16564
                     /gene="rbn"
                     /locus_tag="OO433_002517"
     CDS             15647..16564
                     /gene="rbn"
                     /locus_tag="OO433_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6050950.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         3819 a         4507 c         4259 g         3984 t
ORIGIN      
        1 tgggcgtgga ggaactcgct gcagttaaag aagttctcga atccggttgg atcacaaccg
       61 gtccgaagaa tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca
      121 tcgcggtcag ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca
      181 agggcgatga agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct
      241 tgttgggtgc aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg
      301 aagcgatcga gtcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg
      361 gtgcgccagc agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta
      421 tcgaagatgc tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag
      481 gtaccgctat tttttcattt catgccatta aaaatattac ctgtgcggaa ggtggcctga
      541 ttgtaactga taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggcctgg
      601 gtgtcgatgc ctacgacaga caaacctggg gccgagcacc gcaggctgaa gtcttaacac
      661 cgggctataa gtacaatctg accgatatta acgccgcaat tgccctgaca cagttagcaa
      721 aattagagca cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg
      781 cagctctccc ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc
      841 tgtttattat tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag
      901 cgttaaaaga aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat
      961 attatcgcga gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca
     1021 tctgttcttt gccgctgttc ccggatatga ctaccgccga tgccgaccgt gtcatcacag
     1081 cccttcagca gctcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct
     1141 cggtggttat tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca
     1201 ccacagcctg tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta
     1261 gcgataattc cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt
     1321 ctattttgct taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg
     1381 ttactggcga cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc
     1441 cccgcctagt ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc
     1501 gccaggacag ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca
     1561 ccaccggcaa agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg
     1621 tcgatgcgat gttgcactgc catgaacgta gcacctttat cccgattctg gcgaatatct
     1681 tcgcccgccg tgccattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca
     1741 aatacagttt tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa
     1801 cgccgctacg tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg
     1861 cggtgctgct ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct
     1921 ttatgctatt tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac
     1981 tcggtgaata tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc
     2041 agcaagttat ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta
     2101 ccacgatatg ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc
     2161 catttttacc cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct
     2221 ggcggcggaa agaggcattc cggtttatgc gccggataac gttaatcatc cgctgtgggt
     2281 ggaacgcatt gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat
     2341 ttgcgacgag attttgcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt
     2401 accaaaatat cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac
     2461 gggcgttaca ttgcaccgaa tggtaaaacg tgccgatgct ggggccattg tggcgcaact
     2521 gcgcgttgcc attgcgccag acgatatcgc tattacgctg catcataaat tgtgccatgc
     2581 cacgcgccag ctactggaac agacattacc cgccattaaa cacggtaata ttctggaaat
     2641 cgcccagcgc gaaaacgaag ccacctgttt tggtcgcaga acgccggatg acagtttcct
     2701 tgaatggcat aaaccggcat ccgtactgca caacatggta cgtgccgttg ccgatccgtg
     2761 gccgggtgcc ttcagctatg ttggcaatca gaaattcacc gtctggtcgt cgcgtgttca
     2821 tcctcatgcc agcaaagcac agccggggag cgtgatttct attgcaccgc tgttgattgc
     2881 ctgtggcgat ggcgcgctgg aaatcgtcac cggacaggcg ggcgacggca ttactatgca
     2941 gggctcgcaa ttagcgcaga cgctgggcct ggtgcaaggt tcacgcttga atagccagcc
     3001 tgcctgcacc gcccgacgcc gtacccgggt actcatcctc ggggtgaatg gctttattgg
     3061 caaccatctg acagaacgcc tgctgcgcga agatcattat gaagtttacg gtctggatat
     3121 tggcagcgat gcgataagcc gttttctgaa tcatccgcat tttcactttg ttgaaggcga
     3181 tatcagtatt cattccgaat ggattgagta tcatgtcaaa aaatgtgatg tcgtcttgcc
     3241 gctggtggcg atagccacgc cgattgaata tacccgcaac ccgctgcgcg tatttgaact
     3301 cgattttgaa gagaatctgc gcattatccg ctactgcgtg aagtaccgta agcgaatcat
     3361 cttcccgtca acttcagaag tttatgggat gtgtagcgat aaatacttcg atgaggacca
     3421 ttctaattta atcgtcggcc cggtgaataa accacgctgg atttattcgg tatcaaaaca
     3481 attacttgat cgggtgatct gggcctatgg cgaaaaagag ggtttacagt tcaccctctt
     3541 ccgcccgttt aactggatgg gaccacgact ggataacctt aatgcggcac gaatcggcag
     3601 ctcccgcgct attacgcaac tcattctcaa tctggtagaa ggttcaccga ttaagctgat
     3661 tgatggcgga aaacaaaaac gctgctttac tgatattcgc gatggtatcg aggcgttata
     3721 ccgcattatc gaaaatgcgg gaaatcgctg cgacggtgaa attatcaaca ttggcaatcc
     3781 tgagaacgaa gcgagcattg aggaactggg cgagatgctg ctggcgagct tcgaaaaaca
     3841 tccgctgcgc catcatttcc caccgtttgc gggctttcgc gttgtcgaaa gtagcagcta
     3901 ctacggcaaa ggatatcagg acgtagagca tcgtaaaccg agcatccgca atgcccaccg
     3961 ctgcctggac tgggagccga aaattgatat gcaggaaacc atcgacgaaa cgctggattt
     4021 cttcctgcgc accgttgatc ttacggataa accatcatga ccaaagtagg cttacgcatt
     4081 gatgtcgata cctttcgtgg cacccgtgaa ggcgtgccgc gtctgctgga aatcttgagt
     4141 aagcataata ttcaggccag catttttttc agcgtcggcc cggacaatat gggccgccat
     4201 ctctggcgac tggtgaagcc acagtttttg tggaagatgc tgcgctcaaa cgcggcatcg
     4261 ctttatggct gggatatttt actggcaggt acggcctggc caggtaaaga gattggtcat
     4321 gccaatgccg atatcattcg tgaagcggct aaacatcacg aagtcggcct gcacgcctgg
     4381 gatcaccatg cctggcaagc ccgtagcggt aactgggatc ggcaaacaat gatcgacgat
     4441 attgcacgcg gtcttcgcac tctggaagag attatcggtc aaccggtaac ctgttctgcc
     4501 gctgcgggct ggcgtgccga ccagaaggtg atcgaagcaa aagaagcgtt ccatttgcgc
     4561 tacaacagcg attgtcgtgg ggccatgccg ttccgtccat tgctcgaatc aggaaaccct
     4621 ggcactgcgc aaattccggt gaccttaccc acctgggatg aagtgattgg tcgggatgtg
     4681 aaaacagaag attttaacgg ttggttactc aaccgcatcc tgcgagataa aggcacgccg
     4741 gtttatacca ttcatgcaga agttgaaggc tgcgcttatc agcataattt tgtggatctc
     4801 ctcaaacgcg cagctcagga aggcgtgaca ttttgccctt taagcgaact gttatcagag
     4861 acgttgccgc tcggacaagt tgttcgcgga aatattgccg gacgtgaagg ctggctgggt
     4921 tgccaacaaa ttgcgggtag tcgctgatga aatcggtacg ttaccttatc ggcctcttcg
     4981 cgtttattgc ctgctattac ctgttaccga tcagcacgcg tctgctctgg caacccgatg
     5041 aaacgcgtta tgcggaaatc agtcgagaaa tgctagcatc cggcgactgg attgtgcccc
     5101 atctgttagg gctacgttat ttcgaaaaac ccattgccgg atactggatt aacagcattg
     5161 ggcaatggct atttggcgcg aataactttg gtgtgcgggc aggcgttatc tttgcgaccc
     5221 tgttaactgc cgcgctggtg acctggttta ctctgcgctt atggcgcgat aaacgtctgg
     5281 ctctactcgc cacagtaatt tatctctcat tgtttattgt ctatgccatc ggcacttatg
     5341 ccgtgctcga tccgtttatt gcattctggc tggtggcggg aatgtgcagc ttctggctgg
     5401 caatgcaggc acagacgtgg aaaggcaaaa gcgcaggatt tttactgctg ggaatcacct
     5461 gcggcatggg ggtgatgacc aaaggttttc tcgcccttgc cgtgccggta ttaagcgtgc
     5521 tgccatgggt agcgacgcaa aaacgctgga aagatctctt tatttacggc tggctggcgg
     5581 ttatcagttg cgtactgacg gttctccctt ggggactggc gatagcgcag cgggagcctg
     5641 acttctggca ctattttttc tgggttgagc atattcaacg ctttgcactg gatgatgccc
     5701 aacatagagc tccgttctgg tactacgtgc cggtcatcat tgccggtagc ctgccgtggc
     5761 tgggattact ccccggtgca ctgtacacag gctggaaaaa ccgcaagcat tccgcaaccg
     5821 tctatttgtt gagctggacg ataatgccgc tgctgttttt ctccgtcgct aaaggtaaat
     5881 tgcccaccta tattctttcc tgctttgcat ctctggcaat gctgatggcg cattacgctt
     5941 tgctggcagc aaaaaataat cctctggcgc tgcggattaa tggctggatt aacatcgctt
     6001 ttggcgtcac tggcattatt gccacatttg tggtctcccc gtggggacca atgaacacgc
     6061 cggtgtggca aaccttcgag agctataaag tcttttgtgc ctggtcgatt ttttcgctat
     6121 gggcattttt cggctggtac accttaacaa acgtcgaaaa gacctggtct ttcgccgcgc
     6181 tttgcccgct ggggctggcg ttgctggtag gattttcaat tcctgacaga gtcatggaag
     6241 gaaaacatcc gcaatttttt gtcgagatga cacaagaatc acttcaacca agccgctata
     6301 ttcttactga tagcgtcggt gttgccgcag gtctggcatg gagcctgcaa cgcgatgaca
     6361 tcatcatgta tcgccagaca ggtgagttga aatacggcct taattatccg gatgcgaaag
     6421 ggagatttgt cagcggtgat gagttcgcaa actggcttaa tcaacatcgt caggagggga
     6481 ttattacact cgtgctttcg gttgaccgcg atgaagatat caacagtctc gccattccgc
     6541 ccgcagatgc catcgatcgt caggagcgtc tggtgctgat tcagtatcgt cccaaatgat
     6601 ctggctaaca ttagtctttg ccagcttgct tagcgttgcc gggcagttgt gtcagaaaca
     6661 ggcaacctgc tttgtggcga taaacaaacg gcgcaaacat atcgtgctgt ggctgggact
     6721 ggcgctggct tgtcttggtc ttgccatggt gctctggctg ctggtcttgc agaacgtacc
     6781 ggtaggcatt gcctacccga tgttaagtct gaattttgtc tgggtgacgc tggctgcagt
     6841 aaaactgtgg cacgaaccgg tatcgccgcg tcactggtgt ggggtggcgt tcattattgg
     6901 tggcattgtg atcctcggga gtacggtgta atgggcctga tatggggatt attcagcgtc
     6961 attattgcct cagttgcgca attaagcctg ggttttgcgg caagtcatct gccgccgatg
     7021 acgcacttat gggattttat cgcggctctg ttagcttttg gcctggatgc ccggatactg
     7081 ttactcgggc tgctgggtta tctgctttcc gttttttgtt ggtataaaac gctacataaa
     7141 cttgcgctaa gcaaagccta tgccttgctc agtatgagtt atgtgctggt atggattgct
     7201 tcaatggtac ttcccggctg ggaagggact ttttcgctga aagcactact gggagtagcc
     7261 tgtattatga gcgggttgat gctgattttc ctgcccacga caaaacaacg ttactgagtt
     7321 ttccctgccg ctgtacactg ccgttcccac tcatcggggc tataactgct tgccgagagg
     7381 actttcaccg tatgcagctt ttcacgatta atacaataaa gcgcattctg caaaggcgag
     7441 agtaaatccc ccactttcac ggcgaagtca ctcttcactt cggcgatcat ttttatcgcg
     7501 ccgccagcat cgcagagcat aagtacatgt ctgttatctt gtttattgca gcacgatttt
     7561 ttgaccagcc attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga
     7621 ttaacagata aactctttat caacggcaat ggaatggcag aaatgagaag tgaaacggag
     7681 ggttaattat tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc
     7741 gccgtttttc agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag
     7801 cttatctttc acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac
     7861 cggccgatga ccaaactcct tgtcggcaac gggaacgata aacacctgca aaaccgcagg
     7921 atgtgcagca ataacacgct cgacttcttc cggctgaata ccctcaccgc cactgaataa
     7981 cagattgtct aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg
     8041 cgtagcgtac cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc
     8101 ttctgccata ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc
     8161 cggcagcggc gaaccaacgt ctgccaggcc gtcggcttct ttcgcacata ccgtggaggc
     8221 aaactcggtc agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt
     8281 caactcgacc gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg
     8341 gttaaccagc aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag
     8401 catttgctcc aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg
     8461 ccacataatt ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg
     8521 atcgccaaac ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt
     8581 atggacagcg gctttcggta aaccggtgga gccggaggtc aatgtcattg aacaaagacg
     8641 tgttggttgc cacagagctg aatgtgtgcc ttcaaccatc tgaatatgca gtgaagttaa
     8701 cgccgggaaa gtattttctc cttccagcac cagcgcatat tgcagtgtaa gatttggcag
     8761 taattcctca agcaacggtt gcggaagctg tggatttacg ggcagcaccc gcgccccgca
     8821 ttgcagtaac gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac
     8881 gccgctgccc tcaaccacac cctgcgccgc aaagcccgta gctaactcat cgacgcgagc
     8941 acaaagctcg cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg
     9001 cacttgccgc cagtgacgcc acggccagtc agagaagatc ataacaaccg ctccagtgca
     9061 tcaacttcca cgacaggcag cgtgctaccc ggccagcgac gtacctgctg cgcctgcatc
     9121 agatccagcg tgtccagccc tggaatggtg tccggcgtta accaggcggc aatccgcgcc
     9181 agttgcgtta agcctaagct cgactcaata gaagaactga tcaccgccgt cagcctcaac
     9241 gcgtgcgccg cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg
     9301 ataactaccg cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt
     9361 tcatcccagg caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt
     9421 ttgcacggct cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca
     9481 aactgctgac ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc
     9541 ggaattgcct ccagcaacag attaaccacc atgccgtcgc gcacggcttc gtacaatccc
     9601 actttgacct tcgccacttt ctcgcctggc atatcagcaa gtttgaggat cagatcgtca
     9661 ggatcgccat tacacagtgg tgccgcacgg taattggccg cctgcgggag cgtatctgcc
     9721 agctccgcca atgcacagct tacgccaaag gcaacggaag gcatctgcag tagctcgcaa
     9781 tcgcctgcca gccagttatt tacccaggca agcagcacgc tttgcgcctc ttcccaggtt
     9841 tcctggctga agcccggcag tggggagatc tccccccacc cttcgcgctc gccctcacgc
     9901 aggcaaacgt acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg
     9961 tccatgggga tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt
    10021 tgctgaaatc aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg
    10081 tcatgtagaa cagcatggtg gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac
    10141 agtcggcgtt cagtgcagct ttcaggcagc gcagcgccat cggactgttt tgcagcattt
    10201 cgcggcacca gcgaacggtc tctttttcca gatccgccag cggtaccacg gtgttgacaa
    10261 ggcccatatc cagcgcctgt ttcgcgtcgt actgacggca caggaaccag atttcccgcg
    10321 cttttttctg tccgacgatg cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac
    10381 cgactttcgg gccagtctga ccgaagatgg cattatctgc cgcgatagtc aggtcgcaca
    10441 tcatgtgcag aacgtgaccg ccgccgatgg agtagccagc caccatcgcg acaaccggtt
    10501 tcggacaggt acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat
    10561 catctttata gccgccgtaa tcaccacgca ctttctggtc accgccggag cagaacgctt
    10621 tatcacctgc gccagtcaga atgatcacgc cgacgttgtc gtcataacgc gcatctgcca
    10681 gtgcctggat catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat
    10741 taatggtgat ttttgcgata ccatcggtgg atttttcata acgaatgtcc tcgaaacctt
    10801 cggagcagtc gtgccattca accggtgcgt aaagcattgc ttcatcagga taaatcatat
    10861 tgtgtccttt agtcagaaac gcaagatctg cgccagactt gcgattacgc cagcgggatt
    10921 ttcccgatgc gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc
    10981 cgccagggcg cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg
    11041 ggcgctaagg ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc
    11101 ggcaagggtt gcgccattat tgttgctgcg cagcgccacc agctcccggc gttgatcgtc
    11161 attgagcgag gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc
    11221 tgtgcgaaaa cgctgcgccc attggcgatc ggaacgctga cgttccgcac gttgttcagc
    11281 attttgcagc cccggatgcc cgccttcgac aataacccca caaagccccg ccagcccctg
    11341 gcaagccgcc atcatcgcca cccgtccgcc aagcgagtac cccaccagcc agaagtcaag
    11401 gatgttgtaa ctaaccaagg ttttacgcag taagtcggtg acatcatcaa atccatcgac
    11461 gctaatcgcc gccgaaccac catgacctgg gagatcaaca tacaaccgtg agtagtcggc
    11521 aaacgcctcg cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac
    11581 cagccagggt aaacctggtt ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt
    11641 acctgcgcca gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca
    11701 atcaccgtgg tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag
    11761 ttttgcggac gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt
    11821 tgcggcatca gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt
    11881 tgcccgccgt tgttgttcac cacaattaat accagcggcg cagaaacctg acgcaataac
    11941 gccagcgcgt tgagatcgta aagtgcggag agatcgccca caatcgccag cgtcggtttg
    12001 ccgcttgccc gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct
    12061 cggttgctgt acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc
    12121 accaggctgt taccgacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc
    12181 gccagttgcg cttcgccaaa cgcatcgcga cgggcaataa ccgcctgcat tgcctgttcc
    12241 gccaggcgcg ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc
    12301 cagtcggcaa tattggcaat taagcggcgt ccacgatggt gcgccggatc aagccgccct
    12361 tcgatgtcat ccacaatcca gtactcttcg ggttcacagc ttgcctgcca ttgcaggagc
    12421 cgtttgcccg tcaggctgct tcccagttgc accacaattt gcgcctgctg cagctcgctg
    12481 gtcgctttgg cattgcctaa ccaaagatcg gcacacggca gcggctgccc ggtttgtgac
    12541 agcacatcgc caatcagcgg ccagccaaga gtttgcgccc acagggcaac ttttttgccc
    12601 tcttccgcac tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag
    12661 tcgcgctgtt tttcactttc cagactaggc gcttcacgca gccacggttt gtcgtcctgc
    12721 caccagtcac ccagacgctg ttgccagcta agcccggtat cgtccatttc gccatacagc
    12781 ggttcagcaa acgggcagtt gatatggact ccccccgcat gaagcgtacc gagagcgtgg
    12841 tcgatggtag aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata
    12901 ctgtgcgtgg ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag
    12961 tcaattagct ccggcgggcg atcggcggtt aagagaatca gtttttctcc ggttaacccg
    13021 gcttcaatca gtgccggata gagatttgcc accgccgtgc cggaggtcac aatcaccgcc
    13081 accggctgct tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg
    13141 aaatgggtgt ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa
    13201 cgcgagcctg gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg
    13261 accgccgccc agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt
    13321 gcggctaagt ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac
    13381 tcattactat tccatttgta ataaagtacg caaccctgcc gctttgttgt cgatttcctg
    13441 ccactcttgc tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac
    13501 attgccgcta atttttgctg agcgcaggga aacgcagaat tcgctttgtt gtaatgagag
    13561 atagcccgca gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg
    13621 tcgcgccaga tcgcgcggta agccagcaac tgctgccgtt ggctgcaact gatgtaaaca
    13681 gatcacatca tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt
    13741 acgcagacgc agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg
    13801 ttggcagata tcttccacca ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag
    13861 ccactcttct aactgctgcg cctgcttatc atcaggatga tttgctactg ttcccgccag
    13921 cgcttcagta cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag
    13981 aaaagcattt tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg
    14041 actggcagcc atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg
    14101 agcgagcacc actttgtcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa
    14161 ttgcgtccag cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc
    14221 aggcaagggc ttgatactca ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg
    14281 ctgaagggag ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg
    14341 ccattccagg cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca
    14401 aatgcgtaag tcggcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga
    14461 cgtaaaacgg gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca
    14521 gtagaattgc gggtacgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt
    14581 gagagggaaa ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa
    14641 atggcgcagt agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta
    14701 aaagcctcac attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt
    14761 ttaacggcgt gccagcaaca gtcctagtac cagcccaacg gccgcgccca caccaattcc
    14821 ttgccaaggt ttttcgtgga cgtagtcatc agcacgataa acagcctgct tcgcccgata
    14881 gtaataactg tctgaaacct ggctaacccg ttttttcaca tcatccagcg ctttttctgc
    14941 acgcgctttc agctcaacat atttctgatc agcgggatcg ccagaggagc ggagcacctc
    15001 ttccagtgtc tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt
    15061 agacattctc gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gggttgttga
    15121 ttacgcctga attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac
    15181 ctccgtcaca gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc
    15241 gaggtaaaca ggcttatcag gccagtgatg cgtgcaggtg tcgagggttt tactcatcag
    15301 ttgctgcccc actttttcgc cacgcaacgc ttcactgaca atcacccgac caataacgac
    15361 cggctcaaga tcatcatcac ttttcagaat cctcgcatat gccaccagtt catcattttt
    15421 ccaaccgaga atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg
    15481 gcaattctgc tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga
    15541 aaccgataat tcagagtgat gcagatcttg ccattcaatc ataatgctct ccttttagat
    15601 gtatacctta tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt
    15661 tttaggtact tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa
    15721 cctgcaacat ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca
    15781 tcagctactg cataccgcct ttaaccctgg aaaactggac aagattttta tcagtcacct
    15841 tcatggcgat catctttttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat
    15901 tatccaaccc ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct
    15961 gcggattagc ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga
    16021 aattctcgat gatggcctgc gcaaagtaac cgcttatccg ctggaacacc cactggaatg
    16081 ttatggctat cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa
    16141 agctgctggc gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac
    16201 gctggaagat ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa
    16261 agcgctcgct attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg
    16321 tgtcgatgtc atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag
    16381 tcgcggccat agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggtaa
    16441 gctaatcatt acccacgtca gctcgcgcta tgatgacaaa ggttgtcagc acctgttacg
    16501 tgaatgcagg tcaattttcc cggcgactga actagcgaat gatttcaccg tgtttaacgt
    16561 ttaactctg
//
LOCUS       DAJTSL010000087        16521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTSL010000087 DAJTSL010000000
VERSION     DAJTSL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.087"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            95..2323
                     /gene="crfC"
                     /locus_tag="OO433_002518"
     CDS             95..2323
                     /gene="crfC"
                     /locus_tag="OO433_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6050951.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAAQLAL
                     PWSQPNIAERYAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2320..3203
                     /locus_tag="OO433_002519"
                     /pseudo
     CDS             2320..3203
                     /locus_tag="OO433_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
     gene            3467..4969
                     /gene="proP"
                     /locus_tag="OO433_002520"
     CDS             3467..4969
                     /gene="proP"
                     /locus_tag="OO433_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6050952.1"
                     /translation="MLKRKKVKPITLRDVTIINDSKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5035..5172
                     /gene="pmrR"
                     /locus_tag="OO433_002521"
     CDS             5035..5172
                     /gene="pmrR"
                     /locus_tag="OO433_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6050953.1"
                     /translation="MSDAHTLLQVNGGECNEKPCLYESLTTVFSVLVVSSFLYIWFAT
                     F"
     gene            complement(5148..6248)
                     /gene="pmrB"
                     /locus_tag="OO433_002522"
     CDS             complement(5148..6248)
                     /gene="pmrB"
                     /locus_tag="OO433_002522"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6050954.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6249..6917)
                     /gene="basR"
                     /locus_tag="OO433_002523"
     CDS             complement(6249..6917)
                     /gene="basR"
                     /locus_tag="OO433_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6050955.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6914..8557)
                     /gene="eptA"
                     /locus_tag="OO433_002524"
     CDS             complement(6914..8557)
                     /gene="eptA"
                     /locus_tag="OO433_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6050956.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTV
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8661..9998)
                     /gene="adiC"
                     /locus_tag="OO433_002525"
     CDS             complement(8661..9998)
                     /gene="adiC"
                     /locus_tag="OO433_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6050957.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTPGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10135..10896)
                     /gene="adiY"
                     /locus_tag="OO433_002526"
     CDS             complement(10135..10896)
                     /gene="adiY"
                     /locus_tag="OO433_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6050958.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11221..13488)
                     /gene="adiA"
                     /locus_tag="OO433_002527"
     CDS             complement(11221..13488)
                     /gene="adiA"
                     /locus_tag="OO433_002527"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6050959.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTTDFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     YWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHAMCVKA"
     gene            complement(13687..14595)
                     /gene="melR"
                     /locus_tag="OO433_002528"
     CDS             complement(13687..14595)
                     /gene="melR"
                     /locus_tag="OO433_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6050960.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMMIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14878..16233
                     /gene="melA"
                     /locus_tag="OO433_002529"
     CDS             14878..16233
                     /gene="melA"
                     /locus_tag="OO433_002529"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6050961.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPHIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGESSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            16336..>16521
                     /locus_tag="OO433_002530"
     CDS             16336..>16521
                     /locus_tag="OO433_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6050962.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIM"
BASE COUNT         3987 a         4001 c         4348 g         4185 t
ORIGIN      
        1 tatttcttgc agtaattata aaagccgaaa acagaactca aaaacaatct ggctaaataa
       61 aagtaacaaa atttgcttta aggaagaatt ttctatgtac acacagaccc tgtatgagtt
      121 aagtcaggag gctgaacgcc tgttacagct ttctcgccaa cagttgcagt tactggaaaa
      181 aatgcctctc tctgtacccg gagacgatgc ggcacaactg gctttaccct ggagtcagcc
      241 taatatcgcc gaacgttacg cgatgctgaa taatgagttg cgtaaaattt cccgactgga
      301 aatggtgctg gcgattgtcg gtactatgaa agcagggaaa tcaaccacca ttaatgccat
      361 tgttggtacg gaggttctgc ctaatcgtaa tcgcccaatg actgcgctgc cgacgcttat
      421 tcgccatacg cccgggcaaa aggaaccggt actgcatttt tcacatgtcg cgccaatcga
      481 ttgtttaatt caacaattac aacagcgcct gcgtgattgc gatattaagc atctgaccga
      541 tgtgctggaa atagataaag atatgcgtgc gcttatgcag cggatcgaaa atggcgtcgc
      601 tttcgaaaaa tattatctgg gtgcccagcc tatttttcat tgtctgaaaa gtttgaatga
      661 tttagtgcga ctggcgaagg cgctggacgt cgattttcct ttttctgctt acgccgccat
      721 tgagcatatt cccgtgattg aagtggagtt tgtccatctg gcggggctgg aaagttatcc
      781 cggtcagttg acgttactgg atacccccgg gccaaatgaa gccgggcaac cgcatctgca
      841 aaaaatgctt aaccagcagc tggcacgcgc ctcggcggta ctggcggtgc tggattatac
      901 gcaactgaaa tcgatctccg atgaagaggt ccgtgaggcg attttggcgg tggggcaatc
      961 ggtgccgctg tatgtgctgg tcaataagtt cgatcaacag gatcgtaaca gtgacgacgc
     1021 cgaccaggtg cgggcactga tttccgggac gctgatgaaa ggctgtatta cgccacagca
     1081 gatatttccg gtgtcgtcga tgtggggcta cctggcgaat cgggcgcgct atgagttagc
     1141 caacaacggt aagttaccag cgccagagca acaacgctgg gtggaagatt ttgcccatgc
     1201 cgcgctcggc aggcgctggc gtcatgccga tctggcggac ctcgaacata ttcgtcatgc
     1261 tgccgatcag ttgtgggaag attcgctgtt cgcccagcca attcaggcgt tgcttcatgc
     1321 cgcttacgct aacgcctcat tgtatgctct gcgatctgct gcgcataaac tgttgaatta
     1381 cgcgcagcag gcgcgggaat acctggattt tcgtgcgcac gggttaaacg tcgcttgtga
     1441 acaattgcgg caaaatatcc atcaggtcga agaaagtttg cagctattgc aactcaatca
     1501 ggcgcaggtg agcggcgaga ttaaacatga aatcgagctg gccctgacct ccgccaacca
     1561 ctttctgcgt caacagcaag atgcgctgaa tgcgcagtta gccgccttgt ttcaggatga
     1621 ttcggagccg ttaagcgaga ttcgtacctg ctgtgagaca ctgttacaga cggcgcagaa
     1681 caccatcagt cgcgacttta cgctgcgttt tgccgagctt gaatccaccc tttgccgggt
     1741 gttaaccgat gttattcggc ccattgagca acaagtcaaa atggaattga gcgagtcagg
     1801 gtttcgtcct gggtttcatt ttcctgtttt tcacggagta gttccccacg tcaacactcg
     1861 ccagctgttc agtgaagtca tttcgcgcca ggaggcaacg gacgagcaga gcacgcgttt
     1921 aggcgttgtg cgtgagactt tttcgcgctg gttgaatcag cccgactggg gacggggaaa
     1981 tgagaaatcc ccgacagaaa cggttgatta cagtgtgttg caacgagcat taagcgcaga
     2041 agtcgatctt tattgccaac aaatggctaa agttctggca gagcaggtcg atgaatctgt
     2101 tacggcaggc atgaatactt ttttcgctga gttcgcttca tgtttgacgg aattacagac
     2161 gcgtttacgc gaaagcctgg ctctgcgtca acaaaatgaa tcggtggtca ggctgatgca
     2221 gcagcaattg cagcagactg tgatgactca cggctggatt tacaccgacg cccagctgtt
     2281 acgcgatgat attcaaacac ttttcacggc agaacgatat tgaccaagac gttactggac
     2341 ggccccggtc gcgtgctgga gtcggtttat ccccgctttt tagtggatct ggcgcagggc
     2401 gatgatgccc gccttccaca agcccatcag cagcagtttc gtgaacgact gatgcaggaa
     2461 cttctttcgc gtgtgcagct tcagacatgg acgaacggcg gcatgttaaa tgcgccgctt
     2521 agcctgcgac tgacaatggt ggaaaaactg gcgtcgatgc tggatcccgg tcatctggcg
     2581 ctgacgcaga tcgcgcagca tctggcgctg ctgcaaaaaa tggatcaccg ccagcactct
     2641 gctttcccgg agctccccca gcaaattgcc gccttgtatg agtggttttc agcccgttgt
     2701 cgctggaagg aaaaggcgtt aacgcaacga ggccaggcct actggtgcag gcaggtgatc
     2761 agagcgagca aatttttacc cgctggcgtg ctggggcgta taacgcctgg tcgttgcctg
     2821 ggcgctgttt tatcgttctc gaagagttgc gttggggggc atttggcgat gcctgtcgtc
     2881 tgggaagccc gcaagcggtg gcgttgctgc tgggtgattt gcgcgtgaaa gcgacacaac
     2941 atctggcaga gagtatcaat gcggccccga ccacgcgtca ctattaccat cagtggtttg
     3001 cctcttcgac cgttctgacg ggcggggatc atgctgattt tttaagttgg ctgggaaagt
     3061 ggaccacggc agataaacaa cccgtttgct ggtcagtgac ccaacgttgg caaactgtcg
     3121 cgctggggat gccacgactc tgttcagcgc agcgtttggt gggggcaatg gtcgaggaaa
     3181 tcttctctgt aaatttggcg taaataatca gttacatcaa tgagtcctaa acgaaatcca
     3241 tgtgtgaagt tgatcacaaa tttaaatact ggtagggtaa aaaggtcatt aactgcccaa
     3301 ttcaggcgtc aactggtttg attgtacatt ccttaaccgg agggtgtaag caaacccgct
     3361 acgcttgtta cagagattgc atcctgcaat tcccgctccc cttttgcggc cgtcgcgctg
     3421 atttttctgg cgtttgcgga aatgggccaa ctctgcgagg aaagctatgc tgaaaaggaa
     3481 aaaagtaaaa ccgattaccc ttcgtgatgt caccattatt aatgacagta aactgcgtaa
     3541 agccattacc gcagcatcac tgggtaatgc aatggaatgg ttcgattttg gtgtttatgg
     3601 ttttgttgct tacgcattag gtaaagtttt tttcccgggg gctgacccca gcgtgcagat
     3661 ggttgctgca cttgccactt tctccgttcc ctttctgatt cgaccgcttg gcggactctt
     3721 ctttggtatg ttgggcgata aatatggtcg ccagaagatc ctcgctatca ctattgtgat
     3781 tatgtcgatc agtacgttct gtattggctt aataccgtcc tacgacacga ttggtatttg
     3841 ggcaccgatt ctgctgttga tctgtaagat ggcacaaggt ttctcggtcg gcggtgaata
     3901 taccggggcg tcgatatttg ttgcggaata ctcccctgac cgtaaacgtg gctttatggg
     3961 cagctggctg gacttcggtt ctattgccgg gtttgtgctg ggtgcgggcg tggtggtgtt
     4021 aatttcgacc attgtcggcg aagcgaactt cctcgactgg ggctggcgta ttccgttctt
     4081 tattgctctg ccgttaggga ttatcgggct ttacctgcgc catgcgctgg aagaaactcc
     4141 ggcgttccag cagcatgttg ataaactgga acagggcgac cgcgaaggtt tgcaggatgg
     4201 cccgaaagtc tcgtttaaag agattgccac taaatactgg cgcagcctgt tgacatgtat
     4261 tggtctggta attgccacca acgtgactta ctacatgttg ctgacctata tgccgagtta
     4321 tttgtcgcat aacctgcatt actccgaaga ccacggggtg ctgattatta tcgccattat
     4381 gatcggtatg ctgtttgtcc agccggtgat gggcttgctg agtgaccgtt ttggccgtcg
     4441 tccgtttgtg ctacttggta gtgttgccct gtttgtgttg gcgatcccgg cgtttattct
     4501 gattaacagt aacgtcatcg gcctgatttt tgccgggtta ctgatgctgg cggtgatcct
     4561 taactgcttt acgggcgtta tggcttctac cttgccagcg atgttcccga cgcatatccg
     4621 ttacagcgcg ctggcggcgg catttaatat ttcggtgctg gttgccggtc tgacgccaac
     4681 actggcggcc tggctggtcg aaagctcgca gaatctgatg atgcctgcct attacctgat
     4741 ggtagtggcg gtgattggtt taatcaccgg cgtaaccatg aaagagacgg caaatcgtcc
     4801 gttgaaaggt gcgacaccgg cggcgtcaga tatacaggaa gcgaaggaaa ttctcgtcga
     4861 gcattacgat aatatcgagc agaaaatcga taatattgac cacgagattg ccgatttgca
     4921 ggcgaaacgt acccgcctgg tgcagcaaca tccgcgaatt gatgaataag ctgaaacgga
     4981 tggcctgatg tgacgctgtc ttatcaggcc aattgaactc ttaaggttca cttaatctct
     5041 gacgcgcata ctctcctcca ggttaacgga ggagagtgca atgaaaaacc gtgtttatat
     5101 gaaagtttaa ctaccgtgtt cagcgtgctg gtggtcagca gctttcttta tatctggttt
     5161 gccacgttct gatctttctt cagccgtacc caggcccgcg tgccggaagt ctcttgccgg
     5221 ttttgcagga aaaactgccc gtgatgcaac tgtgtaatgc ggctgacaat acttaacccc
     5281 agaccaatcc cgccataacg gctgtccata cgtacaaacg ctttactcaa ctccccgcat
     5341 ttactctcat caatacctgg tccttcatct tcaactgcca tgaccgctcc gccatcttct
     5401 tgcagcttaa tcataatgtt gctgccttgc gggctgtaac gatgggcgtt ttctaccagg
     5461 tttcgcaata acatccgcag cagggttgca tcaccctgaa cggtgatgtc ggcggcgctc
     5521 tctggcaata gcagggtttg ctgtcgctgg tcgagcatgg tactgagttc gtcatacgag
     5581 gggagaatga catcttccag cagttttaca tgttgataat taccggaaga aaatgactgt
     5641 ccggcacgcg ccagttgcag cagctgggag acgctctcca tcatctgatc aagccgtgcc
     5701 actaacggtg ctacatcaat gtgatgcgtt ttcgccagca gttccagatg caaacgcacc
     5761 cccgccagtg gcgttcgcag ttcgtgcgcg acgtcagcgg taaacaacct ttcgttatcc
     5821 agcgtgctgg tcaggcgact gaccagatcg ttcaacgccg aaaccaccgc ttcgatttcg
     5881 agggtggcgc tgtgaatggc aatgggcgtt aagttgtcgg cagtgcgcgc ttccagctct
     5941 ttttgcagct ccgccagcgg gcgggtgatg cggcgtaccg cctgatagca gataaatagc
     6001 gtcaggctga ccataaagac gccggggaca atcaggctgg cgaccgcctc gcggatctca
     6061 cgcatgatgt ggcgatcgtt gttgcgattg tcgcgtagcg cctgctcaaa cagctgaatc
     6121 tgctcggtac tttcatgcca tagccagaag acgctgatca gctcaaacac caacaaaatg
     6181 gccccgatag tcaatatcag ccgttggcgc agcgatattg gtcggcgcag aaaacgcatc
     6241 agattcaatt agttttcctc attcgcgacc agcatatagc caaagccgcg cacggtgcgg
     6301 atacgggatt tgcccacttt gtcgcgcaga ttgtggatat gcacttccag ggtgttggtc
     6361 gagggttcat tgtcccagtt atagatgtcg ttgtagagaa tttcccgatg taccggactg
     6421 cctgctttga gcattaaccg tgacagcaga gcatattctt tgggcgtcag aatcaactct
     6481 tcaccgccca tccatacctg acggcgaccc atattcagcg tcagattgcc aacaatcagc
     6541 tcactttcgc cctgattatt atggcgtcgt agcagggcgc ggatacgggc gtgtaactct
     6601 tccagcgcaa aaggcttcac cagatagtca tcggcaccga catccagccc ggcgattttg
     6661 tcggtcagcg tatcgcgagc ggtgaggatc agtaccggca gggtatattt tttctgccgg
     6721 atacgggcga gaaaatgcag tccatcttcg tcgggtaacc ctaaatccag taccaccagg
     6781 ctgtaatgac cggcttcaag gctttgttcc gccatccgcg cggttgtcac gccatcgcac
     6841 gcgtagcctt cggtttgcgc cgccagaatc agtccctgca ataacagcgt atcgtcttca
     6901 acaatcagaa ttttcattta ctcactctcc tgcaagtttg cagaatatca tccgcagcct
     6961 ggtaatactt cgtctcaacg ccagttaatc ccaatagcgt ggagaataaa ttgtcttgtg
     7021 aatagtgttg cgtttgcgcc tgtttttgca ggcagttctg gtcaacctga taccgttttt
     7081 gataatcctc cgacagccac agcagcatcg gcacctgttt ttggctatcc ggggcgatgg
     7141 cataaggcag accgtgcaga tagatgccat tttcacctaa cgattcaccg tggtcagaaa
     7201 gataaaccag gctggtggta aatttatcct gatgttcttt cagcagatta atcgctttat
     7261 caacaatata gtcgacgtaa accagcgtgt tgtcgtaagt gttcaccagt tgctctttgg
     7321 tacaggtctg gatctcattg gtgtcgcagg ttggggtaaa tttcctgaac tgcggcggat
     7381 agcggttgta ataggtcgga ccgtggctgc cgatggtgtg taagacaatc acgccatcac
     7441 cctgcaggtt attgatgtac tcttccagcc cgtggaacag cacttcgtca tagcattcgc
     7501 cgttgatgca ctgaccaggc agattcagcg cggtgacgtt ctggtgaggc acgcggtcgc
     7561 aggcaccttt acagccgcca tcgttgtcat tccacagcac gttgatgccc gctcgctgaa
     7621 tgatatccag cacgccttcc tggtgctgtg ccagctcttc tttgtaatgc tcacgcggca
     7681 tatccgagaa catgcacggt actgaaactg ccgttgccgt gccgcaagat acggtattag
     7741 ggaaatagac cacgttatct ttcgccagcc gcgggttagt ttcacgcggg tagccgttga
     7801 gggagaagtt ctccgcccgc gaggtttcgc cgacaatcag gatggtcaaa tttttacgtt
     7861 tttcgttctg cattaacggg ttgcggtggg cgtcttcacc aattcgcacc agcggtagat
     7921 ttgccagtcg ctgatgggag taccatgacc agctggcaac aatgctgtta gaggggctta
     7981 aggatttcac cagctctttg ttattgcgga acaacgaggc gtagtcttta taaaacagtg
     8041 cggcgaccag caaaatcagt agtacagaaa ccagaatatt ggctccacgg aaaagaacac
     8101 tgcgcagacg cgaggtggca ggtttgattt ttatccagca ggcaatcagc gcagcaagca
     8161 cgccgctgaa tcccagcgtt aataacattt gcggtgtcat cagcgcataa ctttctgccg
     8221 gagtggtatc aataatattg gcaatcatcg agcggtcgat gacgatgccg taagtcatta
     8281 tgaaatattg tgcagccgcg ccaaccagaa taaacaggca ggccagtggt cgattaagcc
     8341 ataagaaaga gcttagtgtc aggacaatat taatcacgct gaaagcgacg accggcatcg
     8401 acaagaaaac cagtacgtta tgcagcgaat ccagcggcag cgcctgcaac acctgtttaa
     8461 aaaaggcaat attcaggcag atagagatat aaaaagcggc caacaatagc caggcgagta
     8521 aattcaaaga gggtcttttt agtaggcgct tcaacatcac ggtgtttcca tcgaacaaag
     8581 tgcgcatatg ctcgcaaagc aaaattaagc caaccttaag ttcttaaggt tggcttttat
     8641 gtttgctgga ttaaggggaa ttaatctttg cttattggtg catctaaggg atacgggttt
     8701 ttatgtagcc ggttgtaatt cagcgcatac atggcggtga tgaccatcag ggtgacaaat
     8761 gaccacataa cctctttcgc tccggacccc accacggccc agatgcagta gaggaaggca
     8821 atggtagtaa ctgccagata tgccgggcgt gctttaccaa agtgaccgtg tccgagcagc
     8881 agtaacgccg cacaggtgta aagatatggc accagtgtaa agatgaccga gacggaagaa
     8941 accagaccga actctttggt cgcgtttggt gaaatgctgc tgagctggaa gatggtcatc
     9001 aaaataccga cgataatcag ccccgccact ggcgtacccg ctttatttac acgggcaaaa
     9061 atcggtggga acagtccgtc atcggcagcg gctttcgccg tttgacccgc cagcaacgtc
     9121 cagccgccca gtgaacctaa gcaacccgca gctgcgcaga aggaaacaat ggccccgggg
     9181 gtgtcaccca acgccatccg tgcggcatca ccgaatggcg aagcagaaac gcgcagtgcg
     9241 gcattaggga tcatccccat aatcgcggtg gtagaaagta catagcaaac ggcggcaatc
     9301 aataccccac caatggtggc gatagggaca ttgcgtttcg ggtttttcac cacacctgcg
     9361 gcaacggagg cactttccac accgatgaac gaccacagcg taacgttaag ggtactttga
     9421 attgcaccga aggtacccag gccgctgacg ttccatgccg ccatataggt ttcaccacgg
     9481 aaccagaacc agccaaatac ggcaatcccg acgatgggga tcagcgccag cacggtggca
     9541 actgcctgca cacgggtgat cattttcgga ccaacaatgt tcagcaggac gaagatccac
     9601 agcaccacga cgcaggtgat ggttaatacc aatggatctt tcagaatcgg gaagaagtaa
     9661 cttaaatatc ctacgccaat gaccaccatg gcgatattgc cgatccagca ggccagccag
     9721 tagaggacgt tggtttgata accgagaaac gggccaaagc agcggcgggc gtaagcgtaa
     9781 gaaccaccag gacttgggtc gaggaacgac attttggcgt ataccatcga gagccccagt
     9841 gcaccgataa tcgtcaccaa ccatccataa atggcaatcc cgccagtgga ggccaggttt
     9901 gcaggtaaca gaaaaacacc tgaccccata atattccccg acaccatcag ggtgacgggg
     9961 attaagccca ctttgtgagc atcagcatcc gaagacataa ttaaactcct gcgaaggcga
    10021 gcttcgtgac aataaattac gtcatatcat acgcctgcat tcgatagttg aattattatc
    10081 gaccgggtta tcgctgatgc cgttattaat atagctcttc gttaaggtta aaaatcaggc
    10141 gacagtgcgt tctctgtgct gactaacata atgcagcggc gtcataccgt agaagtcttt
    10201 aaagacagaa ataaagtacg acgtactgtt gtagccgcag gactgtgata cctgagagat
    10261 atttttaccg tccatcatta attcatttac ggcataacgc atgcggcagg tggtgattat
    10321 ttggctataa ctggtgtttt cgcttttcag ctttttcttt aacagacttg ggctaagaca
    10381 taaacaactg gctaccatac taagattcca gtctttgtga atatcgcttt caataatttg
    10441 ataaacgctg tcacttacac aattacgtaa catatacatc attaatgaaa ggaatttttt
    10501 actgtcgaga aaacgggata gcaccgtgaa taataatgcg cgtgttcgtt ccttttctga
    10561 ctcagtttct gcgggcatca tgctgtgttg cgcggcaacg cgaaagacgt ctggcgtcag
    10621 gcatggcagg gtgagtatgg gcgtagcgct acgttgccat actggtattt gcgagagatc
    10681 tttattcaga aaccggaggt aatctttgat gatgtcgtga ctaatatgag caaccaaagt
    10741 gttgttaaga gaggagacgt cgataatatt attttcacaa tttaataacg ccatatggtt
    10801 agctttaagg ctaataggtt ctttcccatt cacccttatc cagacatctt tttcagtcaa
    10861 taaaacaata caaggttggt cgctgcaaat cctcatacat gtactcctga gtgcgaataa
    10921 aagaccagtt ctcgctaaag caaagcgata ccgagaaatg gttgtataat tgtatgacga
    10981 atataaacat gcataaaaat aacgagtttt actaatatta caaattttta tagtaatttc
    11041 tcatgtacat atttttttac agggtaaata acaggcaaaa aaaagcgcgg ggtggggccg
    11101 cgcaaaaagt atgacatttt tgtttgcagc aatttttatg aaaacgttgc catactcaaa
    11161 aggcctaagg ccaggcggcg taatgttatt taaacaatta cgccttcagc ggaatagtgg
    11221 ttacgctttc acacacatag cgtggtaaat accgtcaata atttcagtcc cttcagtttc
    11281 gtgttcaaat ccagggaaat ggtggtccca ggattgcagc gagcgtaaat aacttacttg
    11341 cggactgttt ttatcgccga agttttcacc agacagcagc atcgggattc ctggcggata
    11401 tgggataact gagtttgccg cgatgcgtcc tggcagattt tcaatggata ccagttcgac
    11461 attgttgtcg acaatcgcgt tgtacgcttc acgcggggtg atttccgcca ccggcaggcc
    11521 ggaataggct tcgttcaacc gtgcgccagg gttgttttct ttcagccagg caaacatggt
    11581 gtcacccaga tcgtgaatcc ccatgttcgc gtaagtgtca ggatattgtt caacaagttc
    11641 cggcatcacc tgcgccagcg gtgtgttggc gtcatagtgg cgtttgaagg agcaaagggt
    11701 gttaaccaga gtcccccatt tcccacgggt tacgcccata gagaacagga acataatttg
    11761 gaagtcagtg gtgcgggtag gcacaatgcc gtggcgacca agccaggcag tgaccagcgc
    11821 cgccggaaca ccggtttctt ccagttcacc atcttcaccc attcccggag caaggatgct
    11881 gactttaatc gggtcgagca tactccagtt atccggaata tctttgaagc cgtgccagct
    11941 ttcgcccgga tgcattaccc agtagtcctg aacggtggtc agcagtttgg ttggtgcgtc
    12001 agcaaagtca taggttttgc cggtttgtgg gtcggtgacg acttctttgt tccacggttt
    12061 gaagaaccag ctaccgtcag cggtgaactc tttatacagc cgcgccatcg cctgacggaa
    12121 atcaaccgct tcgtcgatca cttcctgcgt cagtgacagg ccgctgttgc cgtccatcat
    12181 cgacaccgcc acgtcgttgg atgcgcagat ggcatacagc ggggaggtgg tggcatgcat
    12241 catgtaggcc tggttgaagc gggagaagtt aatcgcccca cgaccttcac gtacatgaat
    12301 ataagaagcc tgtgaaagcg cattcagcag tttgtgggtg gagtgggtgg cgaaaacggt
    12361 aggaccgttg tgatcgccag gttcgccgcg catggcatag tgatcggcat agatcgggtt
    12421 gaaacgtgca tagccgtacc aggcttcgtc aaagtgcaga cgatcggagg ttttttccag
    12481 cagatcctgc gcttctttag cgttataaca cacgccgtca taggtgcagt tggtcaccac
    12541 gcagtaagac ggtttttgcc cggctttgtc tttagtcagc gggctttcac tgattttctt
    12601 ctgcaaggtt tcaggttgca tttcctgcgg atagattggc ccgataatgc cgtagcggtt
    12661 gcggcttggc accatgtaga ccggtttcgc gcctgtcagc atcaagcctt gttcgatgga
    12721 tttatggcag ttacggtcaa cgaccacgac atcgttatcg gtcatgcaag cctgcatgat
    12781 ggtgcggtta gagccggaag taccgacgac taccgaccag gagcgatcgg caccaaatac
    12841 gcgtgcggca tatttttcgc tttcgccaaa tgcgccagta tggtcaagca aagaaccgag
    12901 ggaagttcgt tcgatgccca tatcggtgcg gaacagattt tctccatagt agtcatggta
    12961 gaaacgtccg gcgggtgttt tggtaaaacc aacgccgccc tggtggcctg gcgctgccca
    13021 ggaatattca tggatgtcgc tatacttcat cagcgcgctg aacagtggcg gcaacagctg
    13081 ctggcggtag cgggtcatcg cggcaacggc gcgtccggcg ataaagtcgg tggtatcttc
    13141 cagaatccag gcgaattcat cgacaagctc cagcaggtcg cgatccattg cggcgagggc
    13201 tttttcccga tcgcccaaca ggaagaccgg cacgttttgt tggcgctcat gaagcttacc
    13261 gatcaattgt ctgacgtttt gatgttcgtc cggatgttcc atttgatagc tgaacatcag
    13321 gcagtcaatg gcttcgtttg aagagagaat ggcaaaacca tcatcaaagg aggtggattt
    13381 aatcacggta acattttgct ggcttaaagc atctgccaga cgctcaacgg cgttaccgac
    13441 ccaggtgtct tgatggagaa actcgctttc aacaattaat actttcatca ttgcttaccc
    13501 ggttatgaag gaatcttcgt tgcgggcaag tatcttccgg ccttttcggg ctgtaaagcg
    13561 cgtgaaaaac aaacattttt gaaatattta tttttataaa caatgcgtta tgtgattatt
    13621 ttaaattatt ttcactgctt gttatcgacg gctaaactat ttttttggct gatactgata
    13681 tcgtctttag ccgggaaacg tctggcggcg ctgttggcta agtttgcggt attgttgcgg
    13741 cgacatgccg acatatttgc cgaacgtgct gtaaaaacga ctacttgaac gaaagcctgc
    13801 cgtcagggca atatcgagaa tacttttatc ggtatcgctc agtaacgcgc gaacgtggtt
    13861 gatgcgcatc gcggtaatgt actgtttcat cgtcaattgc atgacccgct ggaatatccc
    13921 cattgcatag ttggcgttaa gtttgacgtg ctcagccaca tcgttgatgg tcagcgcctg
    13981 atcatagttt tcggcaataa agcccagcat ctggctaaca taaaattgcg catggcgcga
    14041 gacgctgttt ttgtgtgtgc gcgaggtttt attgaccaga atcggttccc agccagagag
    14101 gctaaatcgc ttgagcatca ggccaatttc atcaatggcg agctggcgaa tttgctcgtt
    14161 cggactgttt aattcctgct gccagcggcg cacttcaaac gggctaagtt gctgtgtggc
    14221 cagtgatttg atcatcatgc cgtgagtgac gtggttaatc aggtctttat ccagcggcca
    14281 ggagagaaac agatgcatcg gcagattaaa aatcgccatg ctctgacagg ttccggtatc
    14341 tgttagttgg tgcggtgtac aggcccagaa cagcgtgata tgaccctgat tgatattcac
    14401 tttttcattg ttaatcaggt attccacatc gccatcgaaa ggcacattca cttcgacctg
    14461 accatgccag tggctggtgg gcatgatatg cggtgcgcga aactcaatct ccatccgctg
    14521 gtattccgaa tacagcgaca gcgggctgcg ggtctgtttt tcgtcgctgc tgcacataaa
    14581 cgtatctgta ttcatggatg gctctctttc ctggaatatc agaattatgg caggagtgag
    14641 ggaggatgac tgcgagtggg agcacggttt tcaccctctt cccagagagg cgaggggagc
    14701 gtccgagcat catgaggccg aaaactctgc ttttcaggta acttattacc ataaactcag
    14761 atttactgct gcttcacgca ggatctgagt ttatgggaat gctcaacctg gaagccggag
    14821 gttttctgca gattcgcctg ccatgatgaa gttattcaag caagccagga gatctgcatg
    14881 atgactgcac ccaaaattac atttatcggc gctggttcga cgattttcgt taaaaatatt
    14941 cttggtgatg tgttccatcg agaggcgctg aaaacggcgc atattgccct gatggacatt
    15001 gatcccaccc gcctggaaga gtcgcatatt gtggtgcgta agctgatgga ttcagcaggg
    15061 gccagcggca aaatcacctg ccacacccaa cagaaagaag ccttacagga tgccgatttt
    15121 gtcgtggtgg catttcagat tggcggttat gaaccttgca cggtgactga tttcgaggtc
    15181 tgtaagcggc atggtctgga acaaaccatt gccgatacgt tggggccggg cggtattatg
    15241 cgcgcgctac gtaccattcc gcatctgtgg caaatttgcg aggacatgac ggaagtctgc
    15301 cccgatgcca cattgctcaa ctacgttaac ccaatggcga tgaatacctg ggcgatgtat
    15361 gcccgctatc cgcatatcaa acaggtaggg ctgtgccatt cggtgcaggg aacggcggaa
    15421 gagctggcgc gtgacctcaa tatcgatccg tccacgctgc gttaccgttg cgctggtatc
    15481 aaccatatgg cgttttacct ggaactggag cgtaaaaccg ccgacggcag ttacgtgaat
    15541 ctctacccgg aactgctggc ggcttatgaa gcagggcagg caccgaagcc acatattcat
    15601 ggtaatactc gctgccagaa tattgtgcgc tacgaaatgt tcaaaaagct gggctacttc
    15661 gtcacggaat cgtcagaaca ttttgccgaa tatacgccgt ggtttattaa gccaggtcgt
    15721 gaggatttga ttgagcgtta taaagtaccg ctggatgagt acccgaaacg ctgcgtcgag
    15781 cagctggcga actggcataa agagctggag gagtataaaa acgcctcccg gattgatatt
    15841 aaaccgtcac gggaatatgc cagcacaatc atgaacgcta tctggactgg cgagtcgagt
    15901 gtgatttacg gcaacgtccg taacgatggt ttgattgata acctgccaca aggatgttgc
    15961 gtggaagtag cctgtctggt tgatgctaat ggcattcagc cgaccaaagt cggtacgcta
    16021 ccttcgcatc tggccgccct gatgcaaacc aacatcaacg tacagacgct gctgaccgaa
    16081 gctattctta cggaaaatcg cgaccgtgtt taccacgccg cgatgatgga cccgcatact
    16141 gccgccgtgc tgggcattga cgaaatatat gctcttgttg acgacctgat tgccgcccac
    16201 ggcgactggc tgccaggctg gttgcaccgt taaaacgcga ctaaacgcta ctgcgccggg
    16261 ggatttattc cggcgcacac ctctgacgat accaataaca gaaggcgggc gttggtaaca
    16321 gcgacccgat accctatgag catttcaatg actacaaaac tcagttatgg atttggagcg
    16381 ttcgggaagg attttgcgat cggcattgtg tatatgtacc tcatgtatta ctacaccgat
    16441 gtcgttgggc tgtctgtggg tttggtcggt actttgtttc tggtggcgag gatctgggat
    16501 gctattaacg atccgattat g
//
LOCUS       DAJTSL010000088        16040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTSL010000088 DAJTSL010000000
VERSION     DAJTSL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.088"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(72..317)
                     /locus_tag="OO433_002531"
                     /pseudo
     CDS             complement(72..317)
                     /locus_tag="OO433_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            424..720
                     /locus_tag="OO433_002532"
     CDS             424..720
                     /locus_tag="OO433_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6050963.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(854..1279)
                     /gene="arsC"
                     /locus_tag="OO433_002533"
     CDS             complement(854..1279)
                     /gene="arsC"
                     /locus_tag="OO433_002533"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6050964.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1292..2581)
                     /gene="arsB"
                     /locus_tag="OO433_002534"
     CDS             complement(1292..2581)
                     /gene="arsB"
                     /locus_tag="OO433_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6050965.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2636..2989)
                     /gene="arsR"
                     /locus_tag="OO433_002535"
     CDS             complement(2636..2989)
                     /gene="arsR"
                     /locus_tag="OO433_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6050966.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            3728..3811
                     /locus_tag="OO433_002536"
     CDS             3728..3811
                     /locus_tag="OO433_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6050967.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3865..5217)
                     /gene="gorA"
                     /locus_tag="OO433_002537"
     CDS             complement(3865..5217)
                     /gene="gorA"
                     /locus_tag="OO433_002537"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6050968.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKMLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSEHLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5289..6131)
                     /gene="rlmJ"
                     /locus_tag="OO433_002538"
     CDS             complement(5289..6131)
                     /gene="rlmJ"
                     /locus_tag="OO433_002538"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6050969.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            6334..8376
                     /gene="prlC"
                     /locus_tag="OO433_002539"
     CDS             6334..8376
                     /gene="prlC"
                     /locus_tag="OO433_002539"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6050970.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8384..9136
                     /gene="rsmJ"
                     /locus_tag="OO433_002540"
     CDS             8384..9136
                     /gene="rsmJ"
                     /locus_tag="OO433_002540"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6050971.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVTTPNAVVTKGHRFDIYAGTPV"
     gene            complement(9185..10654)
                     /gene="dtpB"
                     /locus_tag="OO433_002541"
     CDS             complement(9185..10654)
                     /gene="dtpB"
                     /locus_tag="OO433_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR6050972.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINSVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10972..11406)
                     /gene="uspA"
                     /locus_tag="OO433_002542"
     CDS             complement(10972..11406)
                     /gene="uspA"
                     /locus_tag="OO433_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6050973.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11797..12132
                     /gene="uspB"
                     /locus_tag="OO433_002543"
     CDS             11797..12132
                     /gene="uspB"
                     /locus_tag="OO433_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6050974.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12376..13875)
                     /gene="pitA"
                     /locus_tag="OO433_002544"
     CDS             complement(12376..13875)
                     /gene="pitA"
                     /locus_tag="OO433_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6050975.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKIPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14107..15309
                     /locus_tag="OO433_002545"
     CDS             14107..15309
                     /locus_tag="OO433_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6050976.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15624..>16040)
                     /locus_tag="OO433_002546"
     CDS             complement(15624..>16040)
                     /locus_tag="OO433_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HCR6050977.1"
                     /translation="QTRNTFSTKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAP
                     GIILIYLGMICYSIFSKVWLLALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQ
                     TDMGYFIPSRVLVGLGAVCFTLFSIVSILEAGSAKK"
BASE COUNT         4081 a         4093 c         4183 g         3683 t
ORIGIN      
        1 cccccaaacg aatcggaaaa tcgatactgg aaaaactcta actcggtggc cagtttttgt
       61 tgaccacttc acaacaatcc acgtaacatg ggtattgtcg agcaactaca gaacggggta
      121 acggctccca acagagcagc caggagatag cctctccctt ttctggcccc catcatctgc
      181 tggattttat ggtctggcac cttttgtctt atcaggctga caccggcact aatcactata
      241 aacagcaacg aaagctcgac ggcgagaaac acaaacatct ctgcggcatc ttgcagcatc
      301 gcaagccaac tactcatacg taacctcatt ggataaatct atatttccag aataatcgaa
      361 atgaaattca gatcaagata tttctggtat tatcgaaata tcaaccagac agaggggtgt
      421 catatgcagt tggaagaagt cgctaaagcg ctgaaagagc taggtcaccc gacccgtttg
      481 ttcatattta aacatcttgt caaagccggg gaacaagggt tgccggtcgg tgagttgcaa
      541 aagcagcttg ggatacctgg ctccacactc agccaccata tatcagcact ggtatcggta
      601 ggactggtca aacaaaaccg tgagagtcgg acgctgatgt gtgtttcgca atacgcaatg
      661 ctggaagcga taattgaatt cttacgcgag gagtgctgcg taaatagcaa aattgcctga
      721 tgcgctacgc tctacatatt tcatgccata tattgaattt gcacggtttt gtaggtcgga
      781 taagacgcgt tagcgtcgca tccgacataa acaaagcgca ctttaccaac tatcggcagt
      841 ggatatcgcc gctttatttc aggcgcttac ccgcttcatc aaccactttc tcgccatctt
      901 ccttggagaa tgcgcctttt tgcgcatctg gcagaatttc cagcaccact tctgaagggc
      961 ggcacaggcg agttcccagc ggcgtcacca caatcgggcg attaatcaga atcgggtgct
     1021 gaagcataaa gtcgattaac cgatcgtcag taaatttatc ttccgcaagg cccagctcct
     1081 catacggttc gacgttttta cgcagcagcg cgcgtacgga aatccccata tcggcaatga
     1141 gtttgaccag ttcatcgcgc gttggcggag tttccagata atggataatg gtcggttctg
     1201 tgccgctgtt gcggatcatc tccagcgtat tacgcgacgt gccgcaggcc gggttgtgat
     1261 aaatggtaat gttgctcata tcagtatctc attacaaagt gaaagagaga cgtagcgcca
     1321 gcgcagccag cgtcacaaac agcacaggca gggtcatgat aatccctgta cggaagtaat
     1381 atccccagct gatagtcata ttcttctgcg aaagtacgtg cagccagagt agtgtagcca
     1441 ggctaccaat tggggtaatt ttcggtccca aatcgcagcc aatcacattg gcataaacca
     1501 tcgcttcttt gataacgcca gatgccgtgc tgccatcaat ggacaacgcg ccaaccagta
     1561 ccgtcggcat attgttcata atagaagaga ggaaggcggt gaggaatccg gtgccgagcg
     1621 tcgcggccca caggccgtta tccgccagca cgttgagtac gccagaaaga tattccgtta
     1681 atccggcatt gcgcaggcca taaaccacca gatacatgcc gagcgagaag atgacaattt
     1741 gccagggggc accgcgcagg actttacccg tattaatcgc atgaccgcgt ttagcgacga
     1801 caaataatat cagcgcgccc acagctgcaa tggcgctcac cggaatgccg agcggttcca
     1861 ggacgaaaaa tcccaccagc agaagcagta aaacaaccca gccagttttg aacgtagcag
     1921 gatctttgat cgcttctgcg ggagatttca gcagcgccat atcgtagttc tgcggaatat
     1981 ctttgcgaaa atagagatgt agcatcacca gcgtggcaac aatcgcggcg atatccaccg
     2041 gcaccatcac cgaggcgtat tcgcgaaagc cgaggccaaa gaaatcagcg gaaacgatat
     2101 tcaccaggtt ggagacaata agcggcaggc tggcggtatc ggcaatgaat ccggccgcca
     2161 tcacgaacgc cagcgtcgtg cctttactga atcctaaagc cagcagcatg gcgatgacaa
     2221 tcggcgtcaa aataagcgct gcgccatcat tggcaaacag ggcggcaacg gcagcaccga
     2281 gcaggacaat ccaggtaaac agcaagcgac cacgaccatt accccagcgt gagacgtgca
     2341 gcgccgccca ttcaaaaaag ccggactcat ccagcagcag gctgatgata atgacggcga
     2401 taaacgcagc cgtcgcgttc cagacgatat tccacaccac cggaatatca cccggatgga
     2461 ccacgcccgt aactaacgct agtactgcgc cgagcgttgc actccagccg atgcctaaac
     2521 ctttcggctg ccagataacc aatacaatgg tcaggacaaa gatagcgcct gccagtaaca
     2581 taatgcctcc tggataaaac acatgcgaaa atacatatgt gtttagctaa attttttaac
     2641 tgcaaatgtt cttactgtct gcggaacagt tttgtcgagc caggttgcgg acaattgcct
     2701 gaatcttttc ctgttcacat cgccaggctt catcaattat tttcgccgcc catgctggaa
     2761 tatgcggcga taagcggtaa tgaacccatt taccttgctt gcggtccagc aatagcccgc
     2821 tttcacgcag caatgccagg tggcgggaga tcttgggctg cgactggtcg agagcagtgc
     2881 agagatcgca gacgcataac tctcccagtt cgctgagcag taaaacgatg cccagacggg
     2941 tttcatcagc aagaattttg aacaattgga tgggtaacag aaatgacata ttgcgctcct
     3001 gattgttgca ggtagtgtct ctcttcgaag cggataagtc aaaaacatat atgatttaac
     3061 gaatgtgtaa gtgcagagga aggtaatagg tgtgaatttt gagttggcta ttcatttgaa
     3121 aggaggtctg aatcaggacg taaaaaaacc gactttgcgt cggtttttta ctttccagcc
     3181 ctgagttggt ggctctggct ggagtgagag agttcttatc taacagctca atactattag
     3241 ccagtggcta acattgcaag aattagcgat ttcttcagct ggcggaggga gcacttcggt
     3301 atatatcgca gtgacgattt atatactttc actgggtcat cgtcatatta agccttgtcc
     3361 gaatccggat gtaaaaaacc gactttgcgt cggtttttta ctttccagcc ctgagttggt
     3421 ggctctgact ggagtgagat agccatcatc taacgcatca atgctaatac catactgaaa
     3481 ctattgcaag gacgtgctgg ttttataacc tgcatgtact gtatgattat ccagttagct
     3541 ctgaggcatt ttcactctgg caatgcgcat aaacgctttc aaagtcctgg tcagaagtac
     3601 gggtggtgcc gttaactgat gctctggccg gagtgagaga gttcttatct aacaatgaga
     3661 catgcgccgt gacaggcagt ggatgagtaa gcggatgcat tctcactcca tcgcatggag
     3721 aaaacgggtg attgataaag caatcatcgt tctaggggcg ttaattgcgc tgctggaact
     3781 gatccgcttt ctgcttcagc ttctgaactg atagcggaaa cgtaattaag ggctaagagc
     3841 acactactct tagcccttta acatttaacg cattgtcacg aactcttctg ccgccgttgg
     3901 gtgaatggcg acggtattgt cgaagtcttt tttggttgcc cccatcttca gcgccaccgc
     3961 gaagccctgc aacatttcgt ccataccaaa gccaatgccg tgaataccga caatcttctc
     4021 ttccgatcca acgcacacca gcttcatgcg gcacggctgg cggtgagtgg tgacggcggt
     4081 atacatcgcg gtgaaagagg atttatacac tttcacctga tcgtcgccat actgctcgcg
     4141 cgcctgcggt tccgttaaac caacagtacc aatcggcgga tggctgaaga ccacggtcgg
     4201 aatgttgctg taatccagat gctcatccgg cttgttatta aacaggtgtt cagagagacg
     4261 gcgacccgct gcaactgcca ccggtgtcag ctccactgca cccgtgttat cgcccaccgc
     4321 gtaaatacct tcaatgttgg tgttttgata tttatcgacg acgatatagc ctttttcgtt
     4381 ggttttaacg ccagcggctt cgaggttgat gttgtcattg gcaggctcgc gaccaatcgc
     4441 ccaaatcagg caatccaccg tttcactgcg accatcttcc agctccagcg tcaggctacc
     4501 atcggcattt ttcactaccg ctttcgggat ggcgttggtg tgcagctgcg ggccttcggc
     4561 gttcatcact tcgaccagcg tttcggaaat catcgggtcg aagctgcgca gcggcgcatg
     4621 tttacgcaca aacagatgcg ttttcgcgcc gaggccgtta atcacgcccg ccagctcaac
     4681 ggcgatgtaa cccgcgccaa caaccgccac gcgctctggc aaagcaggaa gggcgaagaa
     4741 gccatcagaa tcaataccgt attccacgcc cggaatatcc gggtggctcg gacgaccgcc
     4801 tgtggcgatc agaatatgat cggccgtgat ggtttcgccg tttacctcca gcattttggc
     4861 atcaacgaag cgggcaaagc ctttgattac atcaacgtta tttttaccga gcacgttttc
     4921 ataggaagta tgaatacggt cgatataggc ggtacggctg gcgatcaacg tttcccagtt
     4981 gaacttgttg atggtggtat cgaaaccata atcagggccg tacatatgga tcgcttcacg
     5041 gatttgcgcc gcgtgccaca tcactttttt cggcacgcag ccgacgttca cgcaggtgcc
     5101 gcccagctct ttggcttcaa tcagtgcgca tttctggccg tacatagccg cgcggttgat
     5161 ggaggctata ccgccgctgc cgccgccgat ggcgatgtaa tcatagtgtt tagtcatgac
     5221 agagtgtcct tatcgttgat taccgcgatt gtagcgcgag acgtaatagg tgccagcaat
     5281 ggctgcaatt actccggcac gatccagctt gcggtggcgt gcccggtgcc tgccggaacc
     5341 agtttgctgt gcagccacgg cagcacgtta ttcatctgct gttccagttt ccacggcggg
     5401 ttaatcacaa tcatgccgga agcggtcatg ccacggcgat cgctgtctgg cagtaccgcc
     5461 agttcaattt gcagaatttt gcgaataccg gtcgcttcca gatcgtggat catgcgctta
     5521 atttgctgac gcagcaccac cggataccac agtgcgtaag taccagtggc gaaacgtttg
     5581 taaccttctg ctatcccgct gaccaccgcc tgatagtcgg ttttcatttc atacggcggg
     5641 tcgataagga ttaaaccacg gcgggaaacc ggcggcagtt tggccttaag ctgctggaaa
     5701 ccatcggctt tttcgacgcg cgcacggctg tctttctgaa attcagaacg cagcaacggg
     5761 taatcgctcg ggtgcagttc ggtcagttgc aggctgtctt gttcgcgcag tagctggcga
     5821 gcaatcaatg gcgaaccggg gtagtaacgc aactgaccgc tacggttgaa gtgttttacc
     5881 acattgatgt acgcctccag ttctgcgggc aaatcgtcct gctgccagat acgggcgatg
     5941 ccttcgagat attcgccggt acgctcggca tgttcgctgc ctaactgata acgcccggcc
     6001 cctgcgtggg tgtcgagata gagaaacggt ttatctttct ctttcagcga ctcgatgatc
     6061 aggctctgaa cggtatgttt aaggacgtcg gcgtggttgc cagcgtgaaa gctgtggcga
     6121 taactgagca tgggtaaagg tgttccggta agtaaaaatc ggcccgtatt cggggcgcac
     6181 aaaagcgtat caggacagta taccgaaaag aggccgccgc cgcgaaagcg taacgtttct
     6241 cattgaaatt cactacactt aaccccatgc tacacacatt atgtaaagcg cctgttgagc
     6301 gcttccttaa cctctttaac caggactgcg cgaatgacga atccgttact gactcccttt
     6361 gaattgcctc cgttttctaa aattctcccg gaacatgtcg ttccagccgt gactcaggcg
     6421 ctgaacgatt gccgcgaaaa tgtggagcgc gtagtagcgc aaggggcacc gtacacctgg
     6481 gaaaatctct gccagccgtt ggcggaagtg gacgatgtgt tggggcgtat cttctccccg
     6541 gtcagccacc tgaactcggt gaaaaatagc ccggaactgc gtgaagccta cgagcaaacc
     6601 ctgccgctgc tgtcggaata cagcacctgg gtagggcaac atgaagggct gtataaagcg
     6661 taccgcgacc tgcgcgatgg cgatcattac gccacgctga acacggcgca gaagaaagcg
     6721 gttgataacg ccctgcgtga ttttgaactc tctggcatag gtctgccgaa agagaaacag
     6781 cagcgttacg gcgaaattgc gacccgtctt tctgaactgg gcaaccagta cagcaacaac
     6841 gtcctcgatg cgacaatggg ctggaccaaa ctcgttaccg acgaagcgga gctggcgggg
     6901 atgcctgaaa gcgcgctggc tgcggcaaaa gcccaggccg aagcgaaaga gctggaaggc
     6961 tacctgctga cgctggatat cccaagctac ctgccggtaa tgacctactg cgacaaccag
     7021 gccttgcgtg aagagatgta tcgcgcttac agcacccgcg cctccgatca aggcccgaac
     7081 gccggtaaat gggataacag caaggtgatg gaagagatcc tcgctctgcg tcacgaactg
     7141 gcgcaactgc tgggctttga aaactacgcc tttaaatccc ttgccactaa aatggcagaa
     7201 aacccgcagc aggtgctgga tttcttaacc gatctggcaa aacgcgcgcg tccgcaaggc
     7261 gaaaaagagc tggcacaact gcgcgccttc gccaaagcgg aatttggcgt cgatgagttg
     7321 cagccgtggg atatcgcgta ctacagcgaa aaacaaaaac agcacctcta cagcatcagt
     7381 gacgaacagc tgcgtccgta cttcccggaa aacaaagcgg ttaacggcct gtttgaagtg
     7441 gtgaaacgta tttacggcat caccgctaaa gagcgtaaag atgttgatgt ctggcatccg
     7501 gatgtacgtt tcttcgaact gtatgacgag aacaacgaac tgcgcggcag cttctacctc
     7561 gatctgtatg cccgtgaaaa caagcgcggc ggggcgtgga tggatgactg cgtaggccag
     7621 atgcgtaaag ctgacggttc tctgcaaaaa ccggtcgcgt atttgacttg taacttcaac
     7681 cgcccggtaa atggtaaacc ggcgctgttc actcacgacg aagtgatcac cctgttccac
     7741 gagttcggtc acggcctgca ccatatgctg acccgcatcg aaaccgctgg tgtttccggt
     7801 atcagcggtg tgccgtggga tgcggtcgaa ctgccgagtc agtttatgga aaactggtgc
     7861 tgggagccgg aggcgctggc gtttatctct ggtcactatg aaaccggcga accgctgccg
     7921 aaagagttgc tggataaaat gctggcggcg aagaactacc aggcggcgct gtttattctg
     7981 cgccagctgg agttcggcct gttcgatttc cgcctccatg ccgagttccg cccggatcag
     8041 ggtgcgaaaa tcctcgaaac tctggcagaa atcaagaaac tggttgccgt agtaccgtct
     8101 ccatcctggg gccgtttccc gcacgctttc agccatattt tcgccggtgg ttatgccgca
     8161 ggttactaca gctacctgtg ggctgacgta ctggcggcag atgccttctc gcgctttgag
     8221 gaagagggca ttttcaaccg tgaaaccggg cagtcgttcc tcgacaacat tctgagccgt
     8281 ggcggttcag aagagccgat ggatctgttc aaacgcttcc gtggtcgtga accgcagctg
     8341 gatgcgatgc tggagcatta cggcattaag ggctgatcat tcagtgaaaa tctgcttaat
     8401 tgatgaaaca ggcgccggag acggcgcctt atctgttctg gcggcccgct gggggctgga
     8461 gcacgatgaa gacaacctga tggcgctggt gttaacgccg gaacatctgg aattgcgcaa
     8521 gcgtgatgag ccaaaacttg gcggcatctt tgttgatttt gttggcggag cgatggcgca
     8581 ccgacgcaaa ttcggcggtg gtcgcggtga ggcggtggcg aaagcggtgg gcattaaagg
     8641 cgattatttg ccggatgtgg tggacgccac cgcagggctg gggcgcgatg cctttgtact
     8701 ggcttcagtc ggctgccgcg tgcggatgct ggagcgtaat ccagtggttg ccgcgctact
     8761 cgacgacggc ctggcgcgtg gttatgcgga tgcggaaatc ggcggctggt tgcaggagcg
     8821 gttgcagtta attcacgcct ccagcctgac ggcgctaacc gatattaccc cacgcccgca
     8881 ggtggtctat ctcgatccga tgttcccgca taagcagaaa agcgcgctag tgaaaaaaga
     8941 gatgcgtgtc tttcagtcgc tggtgggacc ggatcttgat gccgatggat tactggagcc
     9001 tgcacgcttg ctggcgacca aacgcgtggt ggtaaagcgc ccggactacg cgccaccgct
     9061 agcgaatgtc accacgccaa acgcggttgt gactaaaggg catcgctttg atatctatgc
     9121 aggtacgccc gtgtaaaagt aaagcagggt agcgataacg ctaccctgct ttctgcaaga
     9181 ataattaatg gctttccggc gtcgcaatca tgcgtttcag ccacggcacc atcaacagca
     9241 tcactactgc aacgcccagc gtgaccagac caatcttacc aaacacgttg gtatagacgg
     9301 gcaacgtctc aagcggatcg gtaatgttgt ccggcaccgc ggtaaatgtt gccacatagc
     9361 cgcccagcaa gaacgcggca gcctgcgtca ggaaccacat cccgagaata aagcccatca
     9421 aatgctgcgg caccagggca gcaatcatcg ccaggccaag ggcgctaata aacagttcac
     9481 ctaagctctg gaataagtac accagcacga taaaccatgg cgatgtcagc ccttgtgcat
     9541 ccgcaaacca cattcccgca gctgccgccg tcaaaaagcc cagtgagcac ataaacatgc
     9601 cgagagtaaa tttcatcggc atcgagaggt ctttgccttt gttacccaga tgcgtgtaaa
     9661 tgcctgccag tattgggctg gcgagtacca cccagaacgg gttaagcgcc tggaagctga
     9721 ccgagttgat ggaaaaaccg agaatttcat gatgcacgtt gttgatggca aagaagttca
     9781 gcgatgttgg catctgggcg tagagaatgt aaaacaccac cgcttcgagc atcaggacaa
     9841 aggcgacaaa cattttattg cgcccggttt tatccagctt gaatgcctga cgaaagaaga
     9901 tgatggtgac gacgatggag agaacaatca gcaccagatt ggcgacttct acgttgtgca
     9961 tcagccatgc acatacgaag atcatcacca cgctgccaag taatacgtac aacagtttgc
    10021 tgaagctcat cggcttgaag tcgggttcag aaccaatgtc tttcaccatt ccacgacagg
    10081 cgatgtaaac cagtaatgcg ataattaacc ccgccccgca caggttgtag gtgactgaat
    10141 aaccgaatct atcagcgatc acaggggcca gcgataacgc tatcaacgag ccgatattga
    10201 tcgacatata gaacagggtg aatgcgccat caagccgcgg atctttcggc ggatagcact
    10261 tcgaaagcaa gctggctggg ttagctttaa acaggccgtt accgacagcg atagtcccca
    10321 gggcgatgaa aatcaggtca ggcttaagta gcgacatgcc ggtcatgaag tagccaatcg
    10381 ccagcacaag tgctccgaga acaatggtgc gtttggtccc cagcaggtgg tcgccgacat
    10441 agccgccaat ggaaatgagg ccatagacca gcgcagcaaa agcaccaaaa gtgacaaaag
    10501 cctgctcttg cgagaatcca agctgtttaa cgaagaaaac cgccagtacg ccctgcacgc
    10561 cgtagtagcc gaatcgctcc cataactcga caaaaaagat catgaaaaat gggcgaggtt
    10621 gctgcagcat ccccatgggt gttgttgtat tcatatttat taaccttcca ataccatcct
    10681 gaaaagtatg aacgcgtaag ggataacgcg cgaaaaagtt tacatctgtc agagattctt
    10741 aatattgatg cggtgaatgg attaatacac tattacaaca gaaaataacc agctaatcac
    10801 actaatgatg gaaaatatga tggtgttaaa tgttgtattg gcaggagatt ttgattgcgt
    10861 acgcagatcg taggccagat aaggggttta cgctgcatca ggtaacaagg cgcgccaaaa
    10921 agcaaaacgc ccgcatatgc gggcgttcag gaacacgtcg tggagggaag attattcttc
    10981 ttcgtcgcgc agcggaacaa tcagcatatc aacgtgaacg gtgttgatca gctgacgtgc
    11041 ggaagacatc agtttgctcc agaagtcctg gtggtgacca caaaccacca gatccatatc
    11101 gtatttcttg attgcatcga ccagaacctg gcccaggtcg ccgctgccgc tcagggtttc
    11161 agtgattggg tagcctgcat tagtggaaag ctcggtcagc gcatgatgtg tctcttcaga
    11221 gatgcgtttc tgcatatcac ccagattcac atcaataagc ccggtgtata ggtcagagta
    11281 gtttacatct acgtggatca gagaaacttt cgcattgtag gggcgagcca tagagactgc
    11341 tttctctacc agaactttgc tttccgggga gagatcgacc gcgatgagaa tgtgtttata
    11401 agccatagtg ttactccttc cataaagttg tcgatgactg gccagctagc gtttcttgtg
    11461 ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc cgtaaagatt
    11521 cagtgttaag accatcctta ccttatagcg acccggatga tccgtcaatc cgccttgctt
    11581 acccgttaga taaacaaatt ttcgacatga atatattgat agtggttaac cttctggaaa
    11641 aaaaacaacc tgatctccta cactatctat agagccgctc ggatgttggt caccacaaaa
    11701 gcggtttctc caggacctgg tgttaactga ttggcagcgt atcgggagcg gtagccccgg
    11761 ggtggaactc cgcgggcagg tcgccgggga ggaggtatga taagcaccgt cgcattattt
    11821 tgggctttat gtgtcgtttg cattgttaac atggcgcgct atttctcatc actacgcgcg
    11881 ttgttagtgg tactgcgtaa ctgcgatcca ttgctctatc aatatgttga tggagggggc
    11941 ttttttacct cacatggcca acccaacaaa caggtgcgtc tcgtttggta tatctatgcc
    12001 caacgttatc gcgatcatca cgatgatgag tttattcgcc gctgtgagcg ggtgcgtcgg
    12061 cagtttattc tgactagcgc attgtgtggt ctggtggtgg tcagcctgat tgcattgatg
    12121 atttggcatt aagcacttca gcaataataa cgcataaaaa aagctggcca gtaaactgac
    12181 ccgctcgaga ctgattacaa acgcaaaatt gcctgatgcg ctacgcttat caggcctgca
    12241 taatctctgc aatctattga atttgcatgt ttttgtaggc cgaataaggc gttcacgccg
    12301 catcaggcat gaacaaagcg cactatgtca caatctgaag cgggccagct gacccgctcg
    12361 ttttggtgcg tacgattaca ggaactgcaa ggagagccag tacagcccgc cggaaagcag
    12421 taccgcagca ggaagggtaa acacccaggc catcagaatg ctggtcacgg ttttacgctg
    12481 taagccgcca ccatctacca ccatcgtccc cgcgacagaa gaggagagta cgtgagtagt
    12541 ggaaaccggc atcccggtat aactcgccag gccgatagac actgccgccg tcatctgggc
    12601 agacatcccc tgagcgtagg tcatgccttt cttaccgatt ttctcaccga tagtcgttgc
    12661 cacacgacgc cagccaatca tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat
    12721 ccacaccggt gcatactcga tggtgctaag catgtcggac ttcagtttct tcaacaggcg
    12781 ctgatcgtca gcactcacgc caggtatctt cactactttg tcgatagtgt cagaaacgca
    12841 cagcataatg cggcgcatct ggctacgttg atcaagcgac agcttgtcgt agctttccac
    12901 atcggtagtt aacatacctt tcaggcggtt gagcgcgtta atggtattcg acggatggca
    12961 atggaactcc gcgggttgcg ttgcgccagc ttccggagcc ggtactaact gatcagcacc
    13021 ggtagcctgt ttcagcagcg caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc
    13081 atcacgggta cgggtgattt cgtagccagt ggcattcata ttcaccacga agcctgctgg
    13141 tgcgacacca atcaatacca acataaccag accaatgcct ttctgaccat cgttcgcgcc
    13201 gtgcgaaaac gccacgccga tagcggaaag gatcagcgca atacgcgtcc agaacggcgg
    13261 ctttttcttg ccgtctttct tttcacgctc cgctggggtc aggtggatac gggcgcgttt
    13321 cttggtgccg ctccagtagc gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc
    13381 gacaataggg gaaacaatca gagaaccgaa aatacttaat actttcggga tattgagtgc
    13441 atccaccact gacgtcccgg tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc
    13501 aatcagtgta tgagagctgg atgcaggtaa accaaagtac caggtaccca ggttccagat
    13561 aatcgccgcc agcaacatag agaacaccat ggcaaggcca tgagacgatc ccatattaag
    13621 cagcagatcc gtcggcagca tatgcacaat ggcataggca acactcagac cacccagcaa
    13681 aacacccaaa aagttgaaca ccgccgccat aaccacggcg agctgagaac gcatcgcgcg
    13741 ggtatagata acggttgcca cggcgttggc tgtgtcatgg aaaccattga tggcttcgta
    13801 gaacagcaca aaagccagtg caagcaataa taacagcccg gtatgcaaat ccaggccagc
    13861 aaacaaatgt agcataggac gttacgccat tttgaggaca tgaacgcggc gcattatcag
    13921 tgactttcgc ggcgcgggca aagtgaaata tagacttttt ttgatttgcc tcctgtatgg
    13981 atttcactca aaaaataatt atcttatata attcaggcaa atacttcctt ttagtaatat
    14041 tgatgctggt gtgaccactg aggaatcttt acaattcacg ctcgtttttt ctaagaggag
    14101 cgcaacgtgg aaaggtttga tgccattatt ataggcgctg gtgcggcggg tatgttctgt
    14161 tctgcgctgg caggtcaggc aggacgccgg gttctgctga tcgataatgg taaaaaacca
    14221 gggcgcaaaa tccttatgtc tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa
    14281 ccaggcgctt atctgagcca gaatccgcat ttttgtaagt ctgcactcgc gcgttttacc
    14341 cagtgggatt tcattgatct ggtcaataaa cacggcatcg cctggcacga gaaaacgtta
    14401 ggacaactct tctgcgatga ctccgcgcag cagattgtcg acatgctggt ggatgagtgc
    14461 gagaagggca atgtaacctt cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa
    14521 acaggcttca cgcttgaact gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg
    14581 accggtgggc tgtcaatgcc ggggctgggc gcatcgccgt ttggttataa gattgccgaa
    14641 caatttggcc tcaacgtgct gccgacccgt gcgggtctgg tgccattcac tctgcataaa
    14701 ccgttgctcg aagagttaca ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct
    14761 gaaaacggca ccgttttccg tgagaactta ctcttcaccc accgcggctt atctggaccg
    14821 gcggtgttgc agatttccag ctactggcaa ccgggtgaat ttgtcagtat caatctgcta
    14881 ccggatgtgg acctcgaaac cttcctgaat gagcagcgta acgcacatcc gaatcagagc
    14941 ctgaaaaaca cactggcggt tcatctaccg aagcggttgg ttgaacgctt acagcaactc
    15001 gggcaaatcc cggatgtttc gctaaaacag ctcaacgtgc gtgaccaaca ggcactgatt
    15061 agcacattga ccgactggcg cgtacaaccc aacggcactg aaggctatcg cactgccgaa
    15121 gtgacgctcg gcggcgtgga caccaacgaa ctctcttcac ggacgatgga agcgcgcaaa
    15181 gtgcctgggc tgtacttcat cggcgaagtg atggacgtta ccggctggct ggggggctat
    15241 aacttccagt gggcgtggtc gagtgcgtgg gcttgtgcgc aggatttgat tgcagcgaag
    15301 tcgtcctgac ctgttaccta aagatgaaaa ttattagagc cgcattaaaa atgagctaat
    15361 tttgatagtg gctatcttgt gattattttc taatgagccc gtgaactgaa accctccagg
    15421 cttaatataa ggtggaagga aagatgattg aaaactcacc cagtggccag atctttataa
    15481 aatatgaaca ggctgaaaaa gacagtaact ttgttgtttt ttatccttaa ccactatgca
    15541 ttaatgctgc gttatttcat gatgcctaag aaaaaccagc gttacgcaaa tggtcaacgc
    15601 tggttttatc cggtacgttg caattatttt ttagcagaac ctgcttctaa tattgaaacg
    15661 attgagaaca acgtaaagca taccgctccc aaaccgacca aaacccgcga aggaataaaa
    15721 tatcccatgt cggtctgcgc catttccgca agaaacgatg ccaggaaaag gcaaaacagg
    15781 caggtgaaga cgggaatcat cggtattctg ttggctaacg aacaggtacg tctccatacc
    15841 agtgccagta gccagacttt tgagaatatg ctgtaacaga tcattccgag gtaaataaga
    15901 ataatgccgg gagccagtcg ggcgcttgca tcggaactga ctaagacgta tatcccctgc
    15961 agtaccgtga ttgagccgag aaaaataacc caataacacc acagccagtg ttctttagtt
    16021 gaaaacgtat tacgtgtttg
//
LOCUS       DAJTSL010000089        15679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTSL010000089 DAJTSL010000000
VERSION     DAJTSL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.089"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..960
                     /locus_tag="OO433_002547"
     CDS             <1..960
                     /locus_tag="OO433_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6050978.1"
                     /translation="IKETELTTNTMKKTKSQLKTLRIIICESTPISHIRYSDILNYRN
                     ELLHGETLYLDNPRSNKKGRTVRTVDSYIALLCSLLRFAYQSGFISTKPFEGVKKLQR
                     NRIKPDPLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNV
                     RRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFYHREYGRTE
                     KQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTA
                     GANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
     gene            1012..1947
                     /gene="ttcA"
                     /locus_tag="OO433_002548"
     CDS             1012..1947
                     /gene="ttcA"
                     /locus_tag="OO433_002548"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6050979.1"
                     /translation="MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPVYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGIPHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2076..3449)
                     /gene="dbpA"
                     /locus_tag="OO433_002549"
     CDS             complement(2076..3449)
                     /gene="dbpA"
                     /locus_tag="OO433_002549"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6050980.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3895..4878)
                     /gene="zntB"
                     /locus_tag="OO433_002550"
     CDS             complement(3895..4878)
                     /gene="zntB"
                     /locus_tag="OO433_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6050981.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5133..6365
                     /gene="dgcM"
                     /locus_tag="OO433_002551"
     CDS             5133..6365
                     /gene="dgcM"
                     /locus_tag="OO433_002551"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCR6050982.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6386..6949)
                     /gene="smrA"
                     /locus_tag="OO433_002552"
     CDS             complement(6386..6949)
                     /gene="smrA"
                     /locus_tag="OO433_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR6050983.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIHQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(7279..8187)
                     /locus_tag="OO433_002553"
     CDS             complement(7279..8187)
                     /locus_tag="OO433_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6050984.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTPSLITQFRRECGYLQS"
     gene            8363..9663
                     /gene="abgA"
                     /locus_tag="OO433_002554"
                     /pseudo
     CDS             8363..9663
                     /gene="abgA"
                     /locus_tag="OO433_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
     gene            9663..11108
                     /gene="abgB"
                     /locus_tag="OO433_002555"
     CDS             9663..11108
                     /gene="abgB"
                     /locus_tag="OO433_002555"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR6050985.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGYIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANFPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFTKQIQ
                     ATLPPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTLSSLK"
     gene            11145..12671
                     /gene="abgT"
                     /locus_tag="OO433_002556"
                     /pseudo
     CDS             11145..12671
                     /gene="abgT"
                     /locus_tag="OO433_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            12682..13197
                     /gene="ogt"
                     /locus_tag="OO433_002557"
     CDS             12682..13197
                     /gene="ogt"
                     /locus_tag="OO433_002557"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6050986.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13392..14144
                     /gene="fnr"
                     /locus_tag="OO433_002558"
     CDS             13392..14144
                     /gene="fnr"
                     /locus_tag="OO433_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6050987.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            14296..15246
                     /gene="uspE"
                     /locus_tag="OO433_002559"
     CDS             14296..15246
                     /gene="uspE"
                     /locus_tag="OO433_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6050988.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFEPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(15296..15554)
                     /locus_tag="OO433_002560"
                     /pseudo
     CDS             complement(15296..15554)
                     /locus_tag="OO433_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
BASE COUNT         4042 a         3906 c         3843 g         3888 t
ORIGIN      
        1 attaaagaga cagagttaac aacaaacaca atgaagaaaa cgaaatcaca attaaaaaca
       61 ctcaggataa taatttgtga gagtaccccg atatcgcata ttcgttatag cgatatctta
      121 aactaccgga atgaactgct gcatggagaa acgctttacc tagataatcc aagatccaac
      181 aaaaaaggaa gaaccgtgcg cacagttgat agctatatcg ccctgctctg ttcgttgtta
      241 cgttttgcgt atcagtcggg atttatatca accaaaccat ttgaaggagt aaaaaaatta
      301 cagcgaaaca gaataaaacc tgacccgtta tctaaaacag aattcaatgc attaatggaa
      361 agtgaaaaag gacagagcca aaacttgtgg aaatttgccg tttactccgg gcttcgtcac
      421 ggggaactgg cagctctggc gtgggaggat gtggatttcg agaagggaat tgtgaatgtc
      481 agaagaaacc tgacgatact tgatatgttc ggtcccccaa aaacaaatgc cgggatccgg
      541 acggtaacat tactgcagcc ggctcttgaa gcactgaagg agcaatacaa actgaccggg
      601 catcatcgca aaagcgaaat cactttttat catcgggagt acggcagaac tgaaaagcaa
      661 aaactgcatt ttgttttcat gcccagggtg tgtaacgaaa aacagaaacc ttattactcg
      721 gtaagcagtt tgggtgcaag atggaatgca gcagtaaaac gtgctggtat tcgccgccgt
      781 aatccgtacc atacgcgaca tacttttgcc tgctggctgt tgacggcagg agcgaacccg
      841 gcatttatag ccagccagat ggggcatgaa actgcgcaaa tggtgtatga aatttacggt
      901 atgtggattg atgacatgaa cgacgaacag atagccatgt tgaatgcgcg gttatcgtag
      961 ttgcaaagtt tgcccccaat ttgccccatt tagtaccaga gaactgaaat aatgcaagaa
     1021 aatcaacaaa ttacaaagaa agaacaatac agcctgaaca aattacaaaa acgtctacgt
     1081 cgtaacgtgg gcgaagccat tgctgacttc aatatgattg aagaaggcga tcgcatcatg
     1141 gtttgcctct cagggggtaa agacagctat accatgctgg agattctgcg caatttgcag
     1201 caaagcgcgc caatcaattt ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc
     1261 ttcccagaac acgttctgcc cgtgtatctt gaaaagctgg gcgttgagta caagattgtc
     1321 gaagagaaca cttacggtat cgtgaaagag aagattccag agggcaaaac cacttgctca
     1381 ctgtgttctc gccttcgtcg cggtatcctt tatcgtaccg caacggaact gggggcgacg
     1441 aagatcgcgc tgggtcacca tcgtgacgat atcctgcaga cgttgttctt aaatatgttc
     1501 tacggcggca agatgaaagg tatgcctccg aaactgatga gcgatgatgg caaacatatc
     1561 gttattcgtc cgctggccta ctgccgcgag aaagatattc agcgatttgc cgatgcaaaa
     1621 gcgttcccga ttattccgtg caacctgtgc ggttcacagc ctaacctgca acgtcaggtg
     1681 attgctgaca tgttgcgtga ctgggataaa cgttatccag ggcgtatcga gacgatgttc
     1741 agcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata ccaacctgtt cgatttcaaa
     1801 ggcatccccc acggttctga agtggttaac gggggtgatc tggcgtttga tcgcgaagag
     1861 atcccactac aaccggcggg ctggcagcca gaagaagatg aaaatcagtt ggatgagtta
     1921 cggctgaatg tggttgaagt gaaataacca ggatagcgcc cgatgcgcaa gcgtatcggg
     1981 ctactcttat ggaggccgga taagacgcgg ccaacgtcgc atccggcaat gccgaagaag
     2041 atgtttactc ttgcacccgg caattcaaca tttcattatt ttaataaccg cacccggcac
     2101 gtttttcctt taatcttccc gccctgtaac tgtttccatg ctttatgagc aacagcctga
     2161 cggaccgcga catagacatg cgccggatgc acggcgattt tgccaatatc tgcgccatca
     2221 agcccgatat ctcctgtcag tgcacctaat acatcacccg ggcgcatttt ggcttttttc
     2281 ccgccatcga tacacaacgt tgccatttct gcttccagcg gcacaatgga actattagct
     2341 ggcggcgttt gccagttaag ttttatctgc aacatgtcag aaatgatatt ggcccgctgt
     2401 gcttcttccg gagcacagaa actgatcgcc agaccgctat ttcctgcacg agctgtacgg
     2461 ccgatgcgat gtacatgaac ttcagggtcc cacgccagct caaagttcac caccagctca
     2521 agcgatttaa tatccagacc acgcgcagca acatcagtcg caaccagtac acgggcgcta
     2581 ccgttagcaa aacgtaccag ggtctgatcg cgatcgcgtt gctccaaatc gccgtgtaat
     2641 gacaatgcac tttgccctac ttcattcagc gcgtcgcaga cagcctggca atcttttttg
     2701 gtattgcaaa acaccacgca agaggatggc tgatgcaagc ttaataaccg ttgcaacaga
     2761 ggaattttgc ctttgctgga tgtctcataa aattgttgtt caatgggtgg caaagcatct
     2821 gttgagtcaa tttcaatcgc caaaggatcg cgttgcactc gtccgctgat tacggcgatg
     2881 gcttccggcc aggttgccga aaacagaagc gtctgtcgag atgcaggcgc aaaacggatg
     2941 acatcatcaa tggcatcgct aaatcccata tccagcatgc ggtcggcctc atccatcacc
     3001 aaagtattca gcgcatccag tgataccgtg cctttttgca agtgatccag caaacgaccc
     3061 ggcgttgcca cgataatatg cggcgcatgt tgcaacgaat cacgctgcat accgaacggt
     3121 tgaccaccgc acaacgtcaa aattttggta tttggcagaa aacgcgccag ccgacgcaat
     3181 tcacctgcca cctgatccgc cagttcacgc gtaggacaca gcactaaagc ctgggtttga
     3241 aatagcgacg catcaatttg ctgtaacaag ccgaggccaa aagccgccgt tttgccgctg
     3301 ccggttttcg cctgcacgcg aacatctttt ccggcaagga tcgccggaag cgcggcggcc
     3361 tgcaccggcg tcatagttaa ataacccaac tcattaaggt tcgtgagttg ggcgggaggc
     3421 aaaacattca gggtagaaaa agcggtcaca atctattctc gtggtcatcg acgcaaagtt
     3481 agcaggcgcg tatcctcgca gatctacgcg ggtggtgggt caggcatggg ttgcgggcga
     3541 gggatcggat cgggcactgg aacaggatcg ccaggaatcg gttcagggac aggaatttgc
     3601 aaataaataa gtgtcgtcat atttccctcc ggtcattggg tggactctta aagggtagac
     3661 gctgataaat aacaggcaaa aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat
     3721 tgtgctatgc agtaattcaa aaaaggaagt aagacaatat ggagcgcaac gcccatcgct
     3781 tgacgttgca ttcacctgca agggagatat tgccctgaat gggtagagag tttattgact
     3841 tcgctcaaac tttgcggcgt ttttgtatac agacagccgg aaaaattgct tttgttacaa
     3901 ccatttacta cgatgcaacc ataaagcaac accaccaata agaacaacta acagaataca
     3961 aaaaattgaa aatccgaatt gccacccgcc gccagggatc ccaccaaggt tgacgccaaa
     4021 taaccctgtc agaaaggtac tgggtaaaaa gaccattgcc atcaatgaca ttgtataggt
     4081 acgacgagct aaattttcct gcatcacctg agcgatttca tccgccatca cgccagtccg
     4141 tgctatacag gcgtcgattt cgtcaagacc gcgcccaagg cgatcggcaa tatcctgcat
     4201 ccgacggcgt tggtcatcgc tcatccacgg caaacgttca ctggcaagac gagcataaac
     4261 atcacgttgc ggtgccatat agcgacgcat cacgattaat tgtttgcgca gcagagccag
     4321 gaatccacgc ggtggaattt gctgatcaag gagattatct tcaaggtcga taattttatc
     4381 gtgcagctgc tcgataaatt cgctggaatg atcggtcaac gcatcgcaca catccaccag
     4441 ccaccctccg caatcggtcg gacccgtgcc ctcttccaga tcgctcacca catcgtccag
     4501 cgccagcact ttgcgttgtc gggtcgaaac aattaaccgc ccgtccatat atacacgcat
     4561 ggcgaccagt tgatcggggc gttcatcggt gctgccgttt atacagcgca atgtaatcag
     4621 cgtgccttca ccgagacggc tgactcgggg ccgtgtgctc tcgcctgcca gcgcatcacg
     4681 tacgttattg ggaagcagcg gtgttgtcgc cagccattgg gcgctatcat ggtgtacata
     4741 attaaggtgg agccagcagg gatgcgcttc atcaatcaca tctgtatttt ccagcggttt
     4801 aacgccgcct ctaccatcca gcatccaggc aaatactgca tccgggacat taacgtccga
     4861 tcccttaatc gcttccacag tgcctccatc atcaacgcat tattttgtag tctagccttc
     4921 tggccctgtt acgcaacatc tcatcacccc attaccctga aatgattaat aaaattctgt
     4981 ctaaattgaa tacaaaaagc aaaatgcttt tccgtataca aaccgtgtga agtgttaaat
     5041 agcgtctatc attatcagaa ttatctgatc atatgacgtg gcttttttgc gatcggatag
     5101 caacaaaaat tgataaaaat aacgggatct caatgattac gcacaacttc aataccctgg
     5161 acttactcac cagtcctgtc tggattgttt cgccctttga ggaacagtta atttatgcca
     5221 atagcgcggc gcgactgttg atgcaagacc tcacgtttag tcagctacga accggaccct
     5281 attccgtctc ctcacaaaaa gaactgccga aatacctctc cgatctgcaa aaccaacacg
     5341 atattatcga aatcctcact gttcagcgta aagaagagga aacagcatta agctgtcggc
     5401 ttgttttgcg agagctgaca gaaacagaac cggtgattat tttcgaaggt atcgaagcgc
     5461 cggcaacgct gggtttaaaa gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt
     5521 tttatgcgcg cttttttctg actaactctg caccaatgtt gttgattgac ccctcacgag
     5581 atggccaaat cgtcgatgct aacctcgccg cgctcaattt ctacggttat aaccatgaaa
     5641 cgatgtgcca gaaacatacc tgggaaatta atatgctcgg gcgtcgcgtc atgcctatca
     5701 tgcatgaaat ttcgcattta cccggcggtc ataaaccttt gaattttgtt cataaactgg
     5761 cggatggatc tactcgtcat gtgcagacct atgccgggcc gattgaaatt tatggcgaca
     5821 agctcatgtt atgtattgtg catgatatta ctgagcaaaa acggctggag gagcagctgg
     5881 aacatgctgc tcaccatgac gcgatgaccg gattactgaa tcggcgacag ttttatcaca
     5941 ttacggaacc aggccaaatg cagcacctcg ccatcgccca ggattacagc ttgttgctca
     6001 tcgacaccga tcgttttaaa cacattaacg atctctatgg gcattctaaa ggtgatgagg
     6061 tgttatgcgc cctcgcccgc accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc
     6121 gttggggagg cgaagagttt gtcttattgc taccaagaac cccactggat accgcgcttt
     6181 cgctggctga aactatccgt gtaagcgtgg ccaaagtgag tatttcgggc ttaccacgtt
     6241 ttaccgtcag cattggtgtg gcgcatcacg aaggaaatga aagcatcgat gaactgttta
     6301 aacgcgttga tgatgctttg tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg
     6361 cataagccgc ggatgcgtct cgagatcaac ggctgcgctt agcgtggcgc tcccagtttt
     6421 cttgcttcgc ctgcgccgtt ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat
     6481 gcggtagcgc ggtgcaatat gcctgaacat catcaaattc ggtcagccag cgcgccacat
     6541 agctgcggac aatattggca tgcgatttat cttcccgccc tttaccatga ataatcagca
     6601 cattacgcaa accatccgcc agggcttggt gaatgaaact gaacaccatt ttgcggcact
     6661 cttccaccgg ctggcgcaaa agattcaggc tcgcctgttg cggatattta ccactgcgca
     6721 gcttatccag caccccatgt tgcaaccctt cccgccgaaa ctccagcggc tgacttagcg
     6781 ggatgatgtc gagaaatccg gtggtgagga aattatcaag ctgcagcgtg tcgatacgct
     6841 gcggcgcacg ttggttacgc gttggatgcc agtggacatc tgtagcacgt ttcagcggct
     6901 ggacatcttc catggcgtca agaaacagcg atttgtcgtc aaggttcatg ttacatcctc
     6961 ccgcaattaa gagcgcgata tgataaccag accgggtcgg tccaacaacg tattaccaaa
     7021 aattccagta ataagttcca aatattgccg atattttaag caaaatgctt atgcatgatt
     7081 attcattcac gatattaata atgtaactta tattttcgtg aaatctgtca ctgaagaaaa
     7141 ttggcaacta aaggttaaaa ccgttataac acagtcaccg gcgcagagga gacaatgccg
     7201 gacttaagac gcggatgcac tgctgtgtgt actgtagagt ctggcggatg tcgacagact
     7261 ctattttttt atgcagtttt aactttgcag atagccgcat tctcgccgga attgcgtgat
     7321 taatgacggg gtcagtggtg tctggcgact atcacgccgc tgaatcaaat aataggccgc
     7381 tttcggtaaa atttcgctaa ccggcaacat caccagcccc tgtccgtgca agggatcgca
     7441 gcccatttct tcaggcagta tgctgagaaa atcgcttttt gccaccagac tgatacaggc
     7501 tgagaacgtc tcgcagacta caccgacctg tggcgtttgc gcctgatcgt caagcaattc
     7561 actcaactgt ttgtagtaac tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa
     7621 ctgtttgatc gaacgggcac caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg
     7681 cttttccagt aatttctcaa aagtaaattc gtggtcgtac ggtccctgat aataggtatt
     7741 gatggtgaaa tccaattctc cctgacgcaa ttcattaatc atcgacacca gttgcccttc
     7801 cataatgcgt acttttacct gcggatgctg ctgatgaaaa cgagatatga cagctggcat
     7861 cagactgcgg gaaatactgg cccccatgcc gatattaatc tgccctgcca gttgcccttg
     7921 tcgttggcga atatcctctt gggctgcgcg cagctcttca agaataagac tggcgtgctg
     7981 ataaaaactt tcaccggcat cagttaacgt cacgccttta ctacggcgaa aaaagagttg
     8041 cgccgctaat ccttcttcca actcctgaat agatttactc agtgccggtt gcgacatatt
     8101 caacattcgg ctcgctccgc gaatgctgcc ctgacgagcc acttcaacaa aagcacgaat
     8161 ttgatgaatt tttacctgaa aagccatgac gccaccgata accgttattt atcagaccaa
     8221 agaaactggc atctacttta atgcagatga ttgtgtcagg gtaatttatg aacggttaaa
     8281 actgtgaaaa atcagttagt gataagtaaa aactatcgct acgtgaaccg ggtcacactt
     8341 tttactgatg acgggaaagg ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc
     8401 aaaattatcg cactggcgac gtgattttca tcactatgca gagtctggct gggtggaatt
     8461 ccgcactgcc acccttgttg cggaagaatt gcagcagctc ggctattcac tggcgctggg
     8521 ccgcgaagta gttaatgaaa gtagccggat gggactacct gatgaactca ctctacaacg
     8581 cgaattcgag cgcgctcgtc aacagggtgc gctagcacaa tggattgcgg tttttgaagg
     8641 tggtttcact ggtatcgtcg ccaccctgga taccggtcgc cccggtccgg tgatggcttt
     8701 ccgtgtcgat atggacgcgc tggatctcag tgaagagcag gatgtcagcc atcgccccta
     8761 ccgcgacggt tttgcgtcat gtaacgccgg aatgatgcat gcctgtggtc atgatggaca
     8821 taccgccatt gggcttgggc tagcgcatac ccttaaacaa ttcgagtccg gactacatgg
     8881 cgtcatcaaa ctgatttttc agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt
     8941 cgatgcaggt gtcgtagatg atgttgatta ttttactgcc gtgcacattg gcactggcgt
     9001 acctgcgggc accgtggtgt gcggcagtga taattttatg gcaaccacca aatttgacgc
     9061 gcacttcacc ggtaccgccg ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt
     9121 gttggcggca gcacaagcca ctcttgcact gcatgcaatc gccccggagc ttccagagta
     9181 aacgtgggcg ttatgcaggc aggaagcggt cgtaacgttg ttcctgcctc ggcgttgctg
     9241 aaagtggaaa cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa
     9301 caagcgattc agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt
     9361 gcagctaccg ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct
     9421 caggtcgcgg ggggcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat
     9481 gccacattaa tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt
     9541 ggcacacagc tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc
     9601 gctattgccg tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc
     9661 tgatgcagga aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata
     9721 ccgatattgc cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag
     9781 cggagcatct ggcttcggcg ctggaatctg caggcttcac cgttacccgc aacgtaggct
     9841 atatcccaaa tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg
     9901 gagaatatga cgccctggca ggtttaagtc agcaagcagg ttgcgcgcaa cctacatccg
     9961 tgacgcccgg tgaaaatggt cacggttgcg gacacaattt gctgggaacc gccgcctttg
    10021 ccgctgcaat agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acggtgcgct
    10081 tttatggttg tcctggcgaa gaaggcggct cgggtaaaac gttcatggtt cgcgaggggg
    10141 tatttgatga tgtggatgcg gcactcactt ggcacccgga agcctttgcc ggtatgttca
    10201 atacccgcac gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg
    10261 ccgcgaattt ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg
    10321 gcaccaactt cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa
    10381 atagcggcgg gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc
    10441 gcgcccccga aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag
    10501 gtgcggcatt gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt
    10561 atctcccgaa tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc
    10621 cggaatggaa ctccgaagaa ctggctttta cgaaacaaat tcaggctacg ctccccccca
    10681 acgatcggca aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt
    10741 ttgcactacg tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg
    10801 ataacgtgct tgcggcatcg actgatgtcg gcgacgtcag ttggaaactg cctgttgccc
    10861 agtgtttcag cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc
    10921 aggggcgaac atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa
    10981 ccacactcaa tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc
    11041 aagttacgga cacgcaaccg tatcactgcc ctatcccaaa aaacgtgaca ctgtcatctt
    11101 taaaataaca acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca
    11161 taccgtcgtc ctcctaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg
    11221 ttccccatcc ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa
    11281 ttttgtcggc ctttggcgtc agtgcgaaaa acccgaccga tggaacgccg gtggtggtga
    11341 aaaacctgct cagtgtggaa ggattacact ggtttttacc caatgttatt aaaaacttta
    11401 gcggttttgc tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc
    11461 gcgtcggctt actgccagcg ctaatggtta aaatggcatc gcatgttaat gtccgctacg
    11521 ccagttatat ggtgctgttt attgcttttt tcagccacat ttcttccgat gcggcgttag
    11581 tgatcatgcc accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag
    11641 gtttactggc tgccattgca ggcgtaggtt acggctttac ggctaattta ctgattgtca
    11701 caaccgacgt gttgctgtcg gggatccgca cggaagcggc ggctgcgttc aatccgcaaa
    11761 tgcacgtcag tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga
    11821 ttgttggcgg cctgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa
    11881 acagcgatga gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag
    11941 gtgtcgtatc gctacttttt attgctgcga tcgcgctgat ggtgatcccg gaaaacggga
    12001 tattgcgcga tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc
    12061 cactaatcat tctttttttc tttgtggttt cgctggctta tggcatcgct acccgcacaa
    12121 ttcgacatca ggcggattta ccgcatttaa tgattgaacc gatgaaagag atggcgggat
    12181 ttatcgtgat ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacatgg
    12241 ggaaattcat cgccgtgggg ctgaccgata tactggaaag ttcagggctt agcggcatcc
    12301 cggcgtttgt cggtctggcg ttgctttcct ctttcttatg catgtttatt gccagtggtt
    12361 ccgcaatctg gtcgattctg gcccccattt tcgtaccaat gtttatgcta cttggctttc
    12421 acccggcatt tgcgcaaatc ctctttcgta ttgccgactc atccgtattg cctttagcgc
    12481 cggtatctcc ttttgttcca ctgtttcttg gattcctgca acgctacaaa ccagacgcga
    12541 aactgggtac ttactattcg ttagtcttgc cctatccgct tatctttttg gtggtatggc
    12601 tgctgatgtt gctggcgtgg tatcttgttg gtctgccgat aggtccgggg atttacccac
    12661 gtttgtctta agagagaacg gatgctgaga ttacttgaag aaaaaattgc cacgccactg
    12721 ggtccactgt gggtgatttg cgatgagcaa tttcgcctgc gggcggttga atgggaagag
    12781 tacagcgaac gcatggtgca gctgctggac atccattatc gcaaagaagg ctatgagcgc
    12841 atttctgcca ccaacccagg cggtttaagc gacaagctta gtgattattt tgccggtaat
    12901 cttagcatta ttgatacgct tcccactgcc acagggggga cgccatttca gcgcgaagtc
    12961 tggaaaactc tacgcactat cccctgcggg caggtaatgc attacggcca actggctgaa
    13021 caattgggcc gccctggcgc ggcgcgtgcc gttggtgcgg caaacggatc gaatcccatc
    13081 agcatcgtcg taccttgcca tcgggttatt ggccgaaacg gcaccatgac cggatatgca
    13141 ggcggagttc agcgaaaaga gtggttattg cgccacgaag gttatctttt gctgtaaaca
    13201 ttaaacaatt tgtgccagct tgttcacact tttatgtaaa gttaccctta acaacttaat
    13261 ggttttcaaa tagatagaca tatatttaca tctaatatcg gaattctctg ctgttaaggt
    13321 ttgcttagac ttacttgctc cctaaaaaga tgttaaaatt gacaaatatc aattacggct
    13381 tgagcagacc tatgatcccg gaaaagcgaa ttatacggcg cattcagtct ggcggttgtg
    13441 ctatccattg ccaggattgc agcatcagcc agctttgcat cccgttcaca ctcaacgaac
    13501 atgagcttga tcagcttgat aatatcattg agcggaagaa gcctattcag aaaggccaga
    13561 cgctgtttaa ggctggtgat gaacttaaat cgctttatgc catccgctcc ggtacgatta
    13621 aaagttatac catcactgag caaggcgacg agcaaatcac tggtttccat ttagctggcg
    13681 acctggtggg atttgacgcc atcggcagcg gccatcaccc gagcttcgct caggcgctgg
    13741 aaacctcgat ggtatgtgaa atcccgttcg aaacgctgga cgatttgtcc ggtaaaatgc
    13801 cgaatctgcg tcagcagatg atgcgtctga tgagcggtga aatcaaaggc gatcaggaca
    13861 tgatcctgct gttgtcgaag aaaaatgccg aggaacgtct ggctgcattc atctacaacc
    13921 tgtcccgtcg ttttgcccaa cgcggcttct cccctcgtga attccgcctg acgatgactc
    13981 gtggcgatat cggtaactat ctgggcctga cggtagaaac catcagccgt ctgctaggtc
    14041 gcttccagaa aagcggtatg ctggcagtca aaggtaaata catcaccatc gaaaataacg
    14101 atgcgctggc ccagcttgct ggtcatacgc gtaatgttgc ctgatttttc cgcataactc
    14161 actatccttc tgtcatatca ttaaattttt ctgatttatt gatctggcag aaggttcatc
    14221 actgtttcat tcaccagata tgggttaatc ttttaattac aaactgcgtt gacagttgtt
    14281 gtaaggagac cctgtatggc tatgtatcag aacatgctcg ttgttatcga tcctaaccag
    14341 gacgaccaac cagcattgcg gcgagctgtt tatttacatc aacggattgg tggcaaaatt
    14401 aaagcctttt tgccgatcta tgacttctca tacgaaatga ccaccctgct ctccccggac
    14461 gaacgtaccg ctatgcgtca gggcgtcatc agccagcgta cagcctggat ccacgagcag
    14521 gcaaaatatt atctcaatgc tggcgttccc attgaaatta aagtggtctg gcataaccgt
    14581 cctttcgaag ccatcattca ggaagtgatc agcggcgggc acgatttggt gctaaaaatg
    14641 gcgcaccaac atgaccgtct ggaagcggtg atttttacgc caacggactg gcatctgtta
    14701 cgcaaatgcc caagcccggt gtggatggtg aaagaccagc cgtggccgga aggaggtaag
    14761 gcgctggtgg cggtgaatct cgccagtgaa gagccgtatc ataatgcgct caatgaaaaa
    14821 ctggtcaaag agacgatcga actggcagaa caagtcaacc ataccgaagt tcatctggtt
    14881 ggcgcttacc cagtaacgcc aattaatatc gcgattgaac tgccggaatt tgagccgagc
    14941 gtttataacg atgccattcg tgggcaacat ttgctggcaa tgaaagccct gcggcagaaa
    15001 ttcggcatta atgaaaacat gacgcacgta gaaaaaggtc tgcccgaaga ggttattcct
    15061 gatttggcgg agcacttaca ggcggggatt gtggttctgg gcacggtagg acgcaccggt
    15121 atttcagcag cattcctcgg caacacggcg gaacaggtga ttgatcatct tcgctgcgat
    15181 ctgttggtca ttaaacctga ccagtatcag acacccgttg aactggatga cgaagaagac
    15241 gattaacgac tcttcaataa ctgacaaggc ccgagttatc gggcctattt tttacttact
    15301 gctcttcgcc gccaaggaaa taaattccca acgggattgc cagcaacaca gtaaagacca
    15361 ggctataaac aaaaatcatc gatgactgaa tcacatacat tgatgtcgtc cactcagaca
    15421 gcggaatatt gtactgttct ataacgccgc cgatagtcgc gcgagtcaca accgacgccg
    15481 caataatgac aaaccgccaa caaaccaaag agaaatttct tccctttcgc tgacttcagt
    15541 ttcgccataa tcatgatgta atccttgtgc agacaaattt cttgttacct aaacgtaaca
    15601 ataccatgac atttcaccgt tgtctgctct gaaaacctca cctcttagct ggatttacaa
    15661 tcaacgcatt gattagtta
//
LOCUS       DAJTSL010000090        15633 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTSL010000090 DAJTSL010000000
VERSION     DAJTSL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.090"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            336..428
                     /locus_tag="OO433_002561"
                     /pseudo
     CDS             336..428
                     /locus_tag="OO433_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            432..611
                     /locus_tag="OO433_002562"
                     /pseudo
     CDS             432..611
                     /locus_tag="OO433_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            945..3224
                     /gene="ydeP"
                     /locus_tag="OO433_002563"
     CDS             945..3224
                     /gene="ydeP"
                     /locus_tag="OO433_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6050989.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHTASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIEHISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3472..3669
                     /gene="safA"
                     /locus_tag="OO433_002564"
     CDS             3472..3669
                     /gene="safA"
                     /locus_tag="OO433_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6050990.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3744..4505
                     /gene="ydeO"
                     /locus_tag="OO433_002565"
     CDS             3744..4505
                     /gene="ydeO"
                     /locus_tag="OO433_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6050991.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            4907..6589
                     /locus_tag="OO433_002566"
     CDS             4907..6589
                     /locus_tag="OO433_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6050992.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYNLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6641..7798
                     /locus_tag="OO433_002567"
     CDS             6641..7798
                     /locus_tag="OO433_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6050993.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CSFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7764..7886)
                     /locus_tag="OO433_002568"
     CDS             complement(7764..7886)
                     /locus_tag="OO433_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6050994.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8095..9780
                     /locus_tag="OO433_002569"
     CDS             8095..9780
                     /locus_tag="OO433_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCR6050995.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            9818..12190
                     /locus_tag="OO433_002570"
     CDS             9818..12190
                     /locus_tag="OO433_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6050996.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVVGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYSGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            12247..15030
                     /locus_tag="OO433_002571"
     CDS             12247..15030
                     /locus_tag="OO433_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6050997.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKKALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            15392..>15633
                     /locus_tag="OO433_002572"
     CDS             15392..>15633
                     /locus_tag="OO433_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6050998.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         4552 a         3398 c         3426 g         4257 t
ORIGIN      
        1 tggtgacggc agttgaaacc aatgtgttga ggttgcatgg acggttaagc cgagaatacg
       61 gtagtaagtg agatatgggg tttggtcgtt gttaatgagc aaccatgacc atgaccgcca
      121 acgactggat tctcccttgg ttatgttggt gcgcataatg tatattatgt taaatcatat
      181 ctgagtgatg cgaagtgctt ttctggtagt cgttattcgt tcagggttcc agaacttaag
      241 cctctcctct ttcataattg gcctgtgcat gacggatata ggctggctgg tatgcagatt
      301 tgccgtttgc cgcatcttcc cagcgttgaa gactcaagct ggcaaaccgc gtgcaaatga
      361 gcacgccctc tttccatcaa cacttcaggc agcttgcggg aatgagtcca ctacggtatc
      421 agaaatggga agtgggtgtc tctctgatgc gtaatggaaa aatactggct acgggagaaa
      481 atgtctccct ggggacagta aataaaagca aagtatctct gggtttaagc gcaacctatg
      541 gacaaacagg aaacaaggtt gcagccggaa ccgtccagtc agtcattggt gtcactttta
      601 tctatgaata attgtgattt tgatcttagt ctatgcgctc cattcacgaa aattgcgcgg
      661 ataattattt tgtgaaggct attatcctac acctgtaaga aaatccgcgc acaattgaag
      721 ccagagtctt attttttata ttgaaaaata tgttaattta aacactatga gagatgatgc
      781 ttaacttatc gtatttaatc tattgtttaa cgataataga ttaaaaatag aaagagagta
      841 gtcccctact cttatatatc catgttggcg ataatccctt tgtatgtact gtgcatcatc
      901 gctattacaa atcctaataa ttcatttcca cacaggataa gtagatgaag aaaaaaattg
      961 aatcctacca gggtgctgca ggtggttggg gtgctgttaa atccgtagcg aatgcagtac
     1021 gtaagcagat ggatatacgc caggatgtta ttgccatgtt tgacatgaat aagccagagg
     1081 gctttgactg tccgggttgt gcatggccag atcctaagca cactgcgtca ttcgacattt
     1141 gtgaaaacgg cgcaaaagca atcgcctggg aagtcacgga taagcaggta aatgcctctt
     1201 tctttgctga gaatacggtt caatcattac ttacctgggg agatcacgag cttgaggctg
     1261 cgggacgtct cactcagcct ttgaaatatg atgccgtcag cgactgttac aagccattaa
     1321 gctggcaaca agctttcgac gaaattggcg cacgccttca aagctatagt gatcccaatc
     1381 aggttgaatt ctatacttcg ggccgcactt ccaatgaagc tgcctttctt tatcagcttt
     1441 ttgcccgtga atacgggagc aataactttc ccgactgctc caacatgtgc catgaaccga
     1501 ccagtgtggg tttggcagcg agtatcggcg tgggtaaagg gactgtgttg ctggaagact
     1561 ttgagaagtg cgatttagtc atttgcattg ggcataaccc tggtacaaac cacccccgca
     1621 tgctgacttc gttgcgcgct ttagtgaaac ggggagcgaa aatgatcgcc atcaatcctt
     1681 tacaggaacg tggcctggag cgatttaccg caccgcaaaa cccgtttgaa atgctgacga
     1741 actctgagac tcagttggcc agtgcgtact acaacgtgcg cattggtggc gatatggcgt
     1801 tgctcaaggg gatgatgcgc ctgttaattg agcgcgatga tgctgcaagt gccgcaggtc
     1861 ggccctcatt gcttgatgac gaatttattc aaacgcatac cgtcggcttt gacgagctac
     1921 gccgtgacgt tctcaattcc gagtggaaag atatcgaaca tatttctgga ctaagtcaga
     1981 cacaaatcgc cgaactggct gacgcatatg ccgctgccga tcgcaccatt atctgttacg
     2041 gaatggggat cactcagcac gaacatggta cccagaatgt acagcaactg gtcaatctgc
     2101 tgttgatgaa aggtaacatt ggcaagcctg gtgcgggtat ctgcccacta cgaggtcact
     2161 ctaatgtaca gggcgaccga accgtcggta tcaccgagaa accgtctgca gagtttctgg
     2221 ctcgcctggg tgagcgctat ggcttcaccc cacctcatgc acctggacat gctgcaattg
     2281 ccagcatgca agcaatatgc acggggcagg ctcgagcatt gatctgcatg gggggcaact
     2341 ttgcactggc aatgccagat cgggaagcga gcgctgtacc gttaacgcaa ttagatttgg
     2401 cggtacacgt agccactaag cttaaccgct ctcatctgct gaccgcacgg catagctata
     2461 ttctgccggt tctggggcgt agcgagattg acatgcaaaa aagcggtgcg caggcggtaa
     2521 ccgttgagga ttcaatgtcg atgattcatg cctcgcgtgg cgtgttaaaa cccgccggtg
     2581 taatgctgaa atcagagtgt gcagtggtcg cgggaatcgc gcaggcagca ctaccccaga
     2641 gcgtggtagc ctgggagtat ctggtggaag attatgatcg cattcgcaat gacattgaag
     2701 ctgtgttacc agagttcgcc gactataacc agcgcatccg acatcctgga ggttttcacc
     2761 tgataaatgc agctgctgaa aggcgctgga tgacgccgtc aggtaaggct aatttcatta
     2821 ccagcaaagg gctgttagaa gatccctctt cagcgtttaa cagtaagctg gtcatggcga
     2881 cagtacgcag ccacgatcag tacaacacga cgatttatgg tatggatgat cgctatcgag
     2941 gggtattcgg tcaacgagat gtggtcttta tgagtgctaa acaagctaaa atttgccgtg
     3001 taaaaaacgg cgaaagagtt aatcttattg cgcttacgcc agacggtaag cgcagctcac
     3061 gccgcatgga tagattaaaa gtggtcattt accctatggc tgaccgctca ctggtgacct
     3121 attttccaga atcgaatcac atgctaactc ttgataacca cgatccatta agtggcattc
     3181 ctggttataa aagtattccg gttgaattag aaccatcaaa ttaattcgcc tcctcatttc
     3241 ttctgctgtc atccgcacag cagaagaatt tctcattgac tattatcgcg caatttgctc
     3301 acattgatca aattaaacca cacactataa gatataaacc tctgcctaca gctgtaagaa
     3361 actccgctca gtactgaagt gccagtccta tttcctcttt tctccagcct gttatattaa
     3421 gcatactgat taacaatttt taacgttatc cgctaaataa acatatttga aatgcatgcg
     3481 accacagtga aaaacaaaat cacgcaaaga gacaactata aagaaatcat gtctgtaatt
     3541 gtggttgtct tattactgac ccttacgttg atagccattt tttcggcaat tgatcagctg
     3601 ggtatttcag aaatgggtcg cattgcaaga gatcttacac atttcattat caatagtttg
     3661 caagactgaa aataaacagc aaattataaa tatgaaatgt taaaaaagta tcgataaaaa
     3721 ctttattgtt ttaaggagat aaaatgtcgc tcgtttgttc tgttatattt attcatcatg
     3781 ccttcaacgc taacatttta gataaagatt acgccttctc tgacggcgag atcctgatgg
     3841 tagataacgc tgttcgtacg cattttgaac cttatgagcg gcattttaaa gagatcggat
     3901 ttactgaaaa taccattaaa aaatatctac aatgcactaa catccagaca gtgacggtgc
     3961 ctgttcctgc gaagttttta cgtgcttcaa atgtaccgac tggattgctt aatgaaatga
     4021 ttgcttatct caactcggaa gaacgcaatc atcataattt ttcagaactt ttgctttttt
     4081 cttgcctgtc tatttttgcc gcatgcaaag gtttcattac actattaact aacggtgtgc
     4141 tatccgtttc cggaaaagtg agaaatattg tcaacatgaa gctggcgcac ccatggaagc
     4201 tgaaagatat ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc
     4261 aagagcaaac gacattctca cagattctat tagatgccag aatgcagcac gcaaaaaatt
     4321 tgatacgcgt agaaggttca gtcaataaaa ttgccgaaca atgtggttat gccagtactt
     4381 cttattttat ttatgcgttc cgcaaacatt tcggcaacag tccgaagaga gtttctaagg
     4441 agtaccgttg tcaaagtcac acgggtatga atacgggcaa cacgatgagt gctttagcta
     4501 tttgattatt tgctaacgag tagtcaacca cgcacgctac gtaagaatta tatggggcag
     4561 ccattccctg ccccgcgttg tttttaggcg atatatttat tgaaataaat aagtgacatc
     4621 catcacatat ttatgcactt gcataacctg ttgcatgatt atttatgttc tcaattcagc
     4681 atcttgtcag taaaatgcaa taatttatta aatatcaata aattagttgt ttatcggcga
     4741 gaaattactt aatagaacag aaagtaatgt caacgcttta tggactgtta tttccctttt
     4801 tttagctaaa tctgctatct ctttatgtga ctaacttcac ttacatccac ttatttctct
     4861 tcgtaaaatt acttcggaat taagtacaat aagaagagga acatttatga agtctgcatt
     4921 aaagaaaagt gtcgtaagta cctcgatatc tttgatactg gcatctggta tggctgcatt
     4981 tgctgctcat gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc
     5041 agactttacg ccgactgaat acagtaccaa aggaaagcca aatattatcg tactaaccat
     5101 ggatgatctt ggttatggac aacttccttt tgataaggga tcttttgacc caaaaacaat
     5161 ggaaaatcgt gaagttgtcg atacctacaa aatagggata gataaagcca ttgaagctgc
     5221 acaaaaatca acgccgacgc tcctttcatt aatggatgaa ggcgtacgtt ttactaacgg
     5281 ctatgtggca cacggtgttt ccggcccctc ccgtgccgca ataatgaccg gtcgagctcc
     5341 cgcccgcttt ggtgtctatt ccaataccga tgctcaggat ggtattccgc taacagaaac
     5401 tttcttgcct gaattattcc agaatcatgg ttattacact gcagcagtag gtaaatggca
     5461 cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa caaacgcgtg actatcatga
     5521 caacttcacc acattttctg cggaagaatg gcaacctcaa aaccgtggct ttgattactt
     5581 tatgggattc cacgctgcag gaacggcata ttacaactcc ccttcattgt tcaaaaatcg
     5641 tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta accgatgagg caattggcgt
     5701 tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt tacctggctt ataatgctcc
     5761 gcacctgcca aatgataatc ctgcaccgga tcaatatcag aagcaattta ataccggtag
     5821 tcaaacagca gataactact acgcttccgt ttattctgtt gatcagggtg taaaacgcat
     5881 tctcgaacaa ctgaagaaaa acggacagta tgataatact attattctct ttacctccga
     5941 taatggtgcg gttatcgatg gtcctctgcc gctgaacggg gcgcaaaaag gctataagag
     6001 tcagacctat cctggcggta ctcacacccc aatgtttatg tggtggaaag gaaaacttca
     6061 acccggtaat tatgacaagc tgatttccgc aatggatttc tacccgacag ctcttgatgc
     6121 agccgatatc agcattccaa aagaccttaa gctggatggc gtttccttgc tgccctggtt
     6181 gcaagataag aaacaaggcg agccacataa aaatctgacc tggataacct cttattctca
     6241 ctggtttgac gaggaaaata ttccattctg ggataattac cacaaatttg ttcgccatca
     6301 gtcagacgat tacccgcata accccaacac tgaggactta agccaattct cttatacggt
     6361 gagaaataac gattattcgc ttgtctatac agtagaaaac aatcagttag gtctctacaa
     6421 tctgacggat ctacagcaaa aagataacct tgccgccgcc aatccgcagg tcgttaaaga
     6481 gatgcaaggc gtggtaagag agtttatcga cagcagccag ccaccgctta gcgaggtaaa
     6541 tcaggagaag tttaacaata tcaagaaagc actaagcgaa gcgaaataac taaaccttca
     6601 tgcggcggaa ttttccgccg ccttattgag cgagatagcg atgcacgtta cagccaagcc
     6661 ctccagtttt caatgtaatc tcaaatgtga ttactgtttt taccttgaaa aagagtcgca
     6721 gtttactcat gaaaaatgga tggatgacag cactctgaaa gagttcatca aacaatatat
     6781 cgcagcgtct ggcaatcagg tctattttac ctggcaaggc ggtgaaccca ctctggctgg
     6841 cctggatttt ttccgtaaag ttattcacta tcaacaacgc tatgcaggcc aaaaacgtat
     6901 ttttaatgca ttacaaacga atggcatttt attgaataat gaatggtgtt cctttctcaa
     6961 agaacatgaa tttctggttg gtatctcgat cgatggcccc caggagttac atgaccgtta
     7021 cagacgcagt aattcaggta acggtacttt tgcaaaagtg atagcagcca tcgagcgtct
     7081 gaaatcatat caagttgagt ttaatacgtt aaccgtcatt aataacgtta atgtccatta
     7141 ccctcttgag gtttatcatt ttttaaaatc tatcggcagt aaacatatgc aatttatcga
     7201 attgctcgaa accgggacgc cgaatattga tttcagtggt catagtgaga acacattccg
     7261 tatcattgat ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa ccatttttat
     7321 gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt cagtttgaaa gctttgtcag
     7381 ccgttttttg gggaatgggc atacaagttg tattttccag gagtcctgca aggataatct
     7441 ggttgttgaa agtaatggag acatttacga atgcgaccat tttgtctatc cacagtacaa
     7501 aattggaaac attaataaat ctgaactcaa aacgatgaac agtgtacaac tgacagcgca
     7561 aaaaaaacgg atttcagcga aatgtcagca atgtgcatat aaacctatct gcaatggcgg
     7621 ttgtcctaag catcgtatta ctaaagtaaa caatgagact gtttcttatt tttgcgaagg
     7681 ttataaaatc cttttttcaa ccatggtacc ttatatgaac gccatggttg agttagctaa
     7741 gaacagagta ccgctttacc acattatgga tgttgcaaaa caaatggaga ataattaata
     7801 acgctctctt ttaactccat tcttagcctc cttcttatga ccttaacaag gcacccgaca
     7861 taaattcctg tcgggtgttt cgtcatcatc atcttccctt gcgaaaatcc tggcttacgt
     7921 ggtatgcgtc agggcattag caataccctt gattatcaat agcataacca taaccataac
     7981 cactgaaaat taaaaatttc tatatatttc atattgttaa atgatactta aataaatttc
     8041 atcagtaaat cattgccata gataatcatt ggatggcaca atcaattttc gctaatgata
     8101 accattccca ttacgttttg tatgttgata gcaaagtatc tctgtctgct gaagcccttt
     8161 tggctgcgta agaataataa aacttcagtg ttgttaatca ttattatcct cgcaatgatc
     8221 cttggcgtgg ttaaaattca ggtctggtta aacgactgga ataatgattt tttcaatgcg
     8281 ctaagccaga aagaaaccga taagctttgg cagcttgttc tttggttccc tgccctgctg
     8341 gggatctttg tattaatatc cgtaaacaaa acatggttaa ttaaactact gactattcgc
     8401 tggcgcgaat ggttaacaga ttactacctt aatcgctggt ttgcagataa aaactactac
     8461 ttcacgcaga tctacggcga gcataaaaat acagataacc ctgaccaacg tatcgctgaa
     8521 gacattctct tactgatcag caaaacgctc agtctttcat ttggcttcat ccagtcactt
     8581 agcatgctga tcacctttac cgttattctt tggcaaagcg cgggtacgct ctcattcact
     8641 gttggtggaa cagaatggaa tatccagggg tatatggtct ataccgtcgt gctcattgtg
     8701 atcggtggaa ctttatttac ccataaggtt ggtaagcgta ttcgtccgct taatgtggaa
     8761 aaacaacgta gcgaagcgac atttcgaact aatcttgtgc agcataataa gcaggcagaa
     8821 ctgattgcgc taagcaatgc tgagtctcta caacgccagg agttgcgcga caattttcat
     8881 accattaaag agaactggca tcgtttaatg aatcgtcagc ggtggcttga ttactggcaa
     8941 aatatttatt cgcgctcgct tagcgttctt ccctactttc tgttattgcc gcagtttatt
     9001 agcgggcaga tcaatctggg cggactgatg aaatcgcgcc aggcatttat gctagtatcg
     9061 aacaatttaa gctggtttat ttataaatat gacgaacttg ctgaactggc tgcggttatc
     9121 gatcgcttgt atgagttcca tcaactcact gaacagcgcc ctacgaataa gcctaaaaat
     9181 tgccaacatg cggtacaagt ggctaatgcg agtattcgta cgcctgataa taagatcata
     9241 ttagagaact tgaactttca tgtttcgcca ggcaaatggc tattactgaa aggctactct
     9301 ggtgcgggaa aaaccacact gcttaaaaca ttatcccact gctggccgtg gtttaaaggt
     9361 gatatttttt ctcctgctga cagttggtat gtgtcacaaa caccgttaat caaaaccggc
     9421 ttactgaaag agattatttg taaagcactt cccctgcccg tagacgataa atcgttgagc
     9481 gaagtactgc atcaggttgg tcttgggaaa ttggctgcgc gtattcatga ccacgatcgc
     9541 tggggagata ttctttccag cggcgaaaaa caacgtatcg ccctggcacg attaatttta
     9601 cgacgtccga aatggatatt tcttgacgaa actacctctc atcttgagga acaagaggct
     9661 atccgcttac tgcgtttagt gcgtgaaaaa ctacccacaa gcggcgtcat tatggttaca
     9721 catcaacccg gtgtctggaa cctggccgat gatatttgtg acattagcgc ggttttataa
     9781 aaacaataaa agccctgctg tacggagata taaataaatg aagcgagttc ttattcctgg
     9841 cgtcatttta tgtggcgctg atgtggcgca ggccgtcgat gacaaaaaca tgtacatgca
     9901 tttttttgaa gagatgacgg tctatgctcc tgtccctgta cccgtaaacg gcaacacgca
     9961 ttacaccagt gaaagcatcg agcgtttacc gaccgggaat ggcaatatca gcgatctgct
    10021 gagaaccaac cctgcggtac gcatggattc aacgcaaagt acctcgttga accagggaga
    10081 tattcgccct gagaaaatct ctattcacgg tgcgtcgccc taccagaatg cctatttgat
    10141 tgacggtatt agtgcaacta ataacctgaa cccagcgaat gagtccgatg ccagtagtgc
    10201 aaccaatatt agcgggatgt cacaggggta ttatcttgat gtcagcttac tggacaatgt
    10261 gacgctttat gacagttttg tgccggttga atttggtcgc ttcaatggcg gggtaattga
    10321 tgcaaagatc aaacgcttca acgctgatga tagcaaggtg aaattgggtt atcgcactac
    10381 gcgttcggac tggttaacat cgcatatcga tgagaataac aagagcgcat ttaatcaagg
    10441 ttcttcagga agtacttatt tctctccaga ttttaaaaag aacttttata ccttgtcgtt
    10501 taatcaggag ctggctgata actttggcgt taccgccggt ttatcgcgcc gccagtctga
    10561 tatcacccgc gcggattatg tttcgaatga cggcattgtc gtcggtcggg cacagtataa
    10621 aaacgttatc gatactgcat tgagcaaatt tacctggttt gccagcgacc gctttaccca
    10681 cgatttaacc ttaaaatata ccggctccag ccgtgattat aataccagca ccttcccgca
    10741 gtctgatcgc gaaatgggta ataaatccta tggtcttgca tgggatatgg atacgcaact
    10801 cgcatgggcc aaactacgta ccaccgttgg ttgggatcat attagtgatt atacccgtca
    10861 cgatcatgac atctggtaca ccgaactttc atgtacatat ggtgatatta cagggcgttg
    10921 tacccgtggc ggattaggac acatttccca ggctgtagat aattacacct tcaaaacacg
    10981 cctggactgg caaaaattcg ccgtgggtga tgtttcgcat caaccctact ccggcgcgga
    11041 atacatctat tccgatgcat ggactgaacg ccataaccag tctgaatcct atgtgattaa
    11101 tgctgcagga aagaaaacta accataccat ttaccataaa ggtaaaggca gcctaggaat
    11161 tgacaactac acgctgtata tggcggatca cattagctgg cggaatgtgt cgttaatgcc
    11221 cggtgtgcgt tatgactatg acaactatct gtcaaaccac aatatctccc cgcgctttat
    11281 gacggaatgg gatatttttg ctgatcaaac ctcaatgatt accgccggtt ataaccgtta
    11341 ctatggcggg aatattcttg atatgggatt acgtgatatc cgcaatagct ggacggaatc
    11401 ggtatcaggt aataaaaccc tgacgcgtta tcagaatttg aaaacgcctt ataacgatga
    11461 actggcaatg ggattgcagc aaaaaatcgg taagaacgtt attgcacgcg caaactatgt
    11521 ttaccgtgaa gcgcatgatc aaatcagcaa aagcagtcgt accgacagcg cgactaaaac
    11581 caccattact gaatataaca acgatggcaa aaccaaaacg cattcgttta acctcagttt
    11641 tgaactggcc gaacccctgc atatccgcca ggtagatatt aacccgcaaa ttgtctttag
    11701 ctatatcaag agcaagggca acttgtcgtt aaacaatggt tatgaggaga gcaataccgg
    11761 tgataaccag gtggtttata acggtaatct ggtctcttac gatagcgttc cagtggcaga
    11821 ttttaataac ccattaaaga tctccttaaa catggatttc acgcatcaac cgagcgggtt
    11881 agtgtgggcg aatacgctgg cgtggcaaga agcgcgtaaa gctcgcatta tccttggtaa
    11941 gacaaatgcg caatacatca gcgaatattc agattacaag cagtatgttg acgaaaaact
    12001 ggatagcagc ctgacctggg acacccgctt gtcctggacg ccacaatttc tgaaacaaca
    12061 aaacctgacg atcagtgccg atattctcaa tgtactggat agcaaaaccg ctgttgatac
    12121 aacgaatacc ggtgtggcga cctacgccag tggccgtact ttctggcttg atgtcagcat
    12181 gaaattttaa tagttcggat tttacttaac cgggtaaatt cgcccggttt tcgcatggag
    12241 ataattatga gaaacctctg tttcttactg acgttagtgg caactctgtt gctccccggg
    12301 cggctgattg ccgccgcctt accgcaggat gaaaagttaa ttaccgggca actggacaat
    12361 ggcttgcgat atatgattta tccgcatgct caaccaaagg atcaggtaaa tttatggctg
    12421 caaattcata ccggttcatt gcaggaagaa gacaatgagc gcggcgtggc tcattttgta
    12481 gaacatatga tgtttaacgg cacaaaaaca tggccgggta ataaagtcat cgaaacattt
    12541 gagtcaatgg gcctgcgttt tggtcgcgat gttaatgcct ataccagcta tgacgaaacg
    12601 gtgtatcagg tgagtttgcc gactacgcag aaacaaaatc tgcaacaagt gatggcaatc
    12661 ttcagtgaat ggagtaatgc cgcaaccttt gaaaaactcg aagtagacgc tgaacgtggc
    12721 gtaattactg aggaatggcg tgcccatcag gatgcgaaat ggcgcacctc tcaggcgcgc
    12781 cgccctttcc tgctggctaa tacccgtaat ttagaccgtg aacctatcgg cctgatggat
    12841 actgtcgcca cggtcacacc ggcacaattg cgccaatttt atcaacgctg gtatcaacca
    12901 aataatatga cctttatcgt ggtcggcgat atcgacagta aaaaagcgct ggcgctgata
    12961 aaggataatt taagtaagct tccggctaac aaagcagctg aaaatcgcgt ctggccgaca
    13021 aaagccgaaa accacctgcg ctttaatatc attaatgata aagaaaaccg ggtgaacggc
    13081 atcgcactct attatcgcct gccaatggta caagtgaacg atgagcaaag ctttatcgaa
    13141 caagctgaat ggagcatgtt agttcagctg ttcaatcaac gtctgcagga acgcatacag
    13201 tcgggcgagt tgaagactat ttctggcggc actgcgcgca gcgttaaaat tgcacccgat
    13261 tatcagtcgc tgtttttccg tgtaaatgca cgagacgata atatgcagga tgctgcgaat
    13321 gcattaatgg cagagttggc aaccattgat cagcatggtt tttctgctga agaactcgat
    13381 gatgtcaaat ctacccgcct cacctggctg aaaaatgcgg ttgatcagca agctgaacgt
    13441 gatttacgta tgctgaccag tcgcctggca tccagctcat taaataatac gccgttcttg
    13501 tcgccggaag agacatatca actttcgaaa cgtctgtggc agcaaattac cgtgcaaagt
    13561 ctggcggaaa aatggcagca gttaagaaag aaccaggacg cattttggga gcaaatggta
    13621 aacaatgagc ttgccgccaa aaaagcattg tctcctgcgg ctatcctggc gctggaaaaa
    13681 gagtacgcca acaaaaagct ggcggcttac atcttcccag gcagaaattt atcgttaaca
    13741 gtagacgctg acccacaggc ggaaattagc agcaaagaaa cgctggctga gaatctgaca
    13801 tcattaacac tttccaatgg tgccagggtt attctggcaa aatccgcggg tgaagagcaa
    13861 aagctacaaa ttactgccgt atcgaataaa ggcgatttaa gtttccctgc gcagcaaaaa
    13921 tcacttatcg cgctggcaaa taaagcagta agcggaagcg gcgttggtga gctctcctct
    13981 tccagcctga aacgctggag tgcggaaaat tcggtaacca tgagcagtaa agtcagtggc
    14041 atgaatacgt tgctctccgt tagcgcgcgg actaataacc ctgaacctgg ttttcagttg
    14101 attaaccagc gaatcaccca cagcacgatt aacgacaata tttgggcatc gctacaaaat
    14161 gctcaaattc aggcgttgaa aacgctcgac cagcgtccag cggagaaatt cgcccagcag
    14221 atgtatgaga cgcgctatgc tgatgaccgc acgaaattac tgcaagaaaa tcagattgta
    14281 cagtttactg ccgcagatgc gctggctgcc gatcgccagt tgttttcatc tccagcggat
    14341 atcacgtttg tcattgtcgg taatgtctca gaagacaaac tcgtggcgtt aattacgcgt
    14401 tacttaggat caatcaaaca ctctgattcg ccattagccg caggtaaacc attaactcgc
    14461 gcgacggaca acgcatcggt tactgtaaaa gaacaaaatg aacctgtggc acaggtttca
    14521 cagtggaagc gttatgattc ccggacacct gttaatctgg cgacgcgtat ggcgctcgat
    14581 gcttttaacg tcgcactggc aaaagatcta cgtgttaata ttcgtgaaca ggcatctgga
    14641 gcatacagcg tttcttctcg cctctcggtt gatcctcagg ccaaagatat cagtcatttg
    14701 ctggctttta cttgtcaacc agaacgacat gatgaactgt taacgttggc gaatgaagtg
    14761 atggttaagc gtctggctaa agggatcagt gagcaagaac tgaatgaata ccagcaaaac
    14821 gttcagcgca gcctcgatat ccaacagcgt agcgttcaac aattagcgaa cactattgta
    14881 aatagtctta ttcaatatga cgatcctgca gcatggactg agcaggagca attgttgaaa
    14941 caaatgacgg tagagaatgt taacactgcc gttaaacaat atctttctca tccggtaaat
    15001 acttataccg gagtattatt gccaaaataa taacagcccc gtcaacacat cgttggcggg
    15061 gattttagca atattcgcta tttttatgta ataattttat aaatgcgttc aaaataataa
    15121 tcaagtacta atagtgatat tttaaggtct gatttttacg tgataattca ggagacacag
    15181 aatgcgcata aaaataacag cataaaacac cttaccacca cccaagaatt tcatattgta
    15241 ttgtttttca atgaaaaaac attattcgcg taatatctca cgataaataa cattaggatt
    15301 ttgttattta aacacgagtc ctttgcactt gcttacttta tcgataaatc ctactttttt
    15361 aatgcgatcc aatcatttta aggagtttaa aatggataag aagcaagtaa cggatttaag
    15421 gtcggaacta ctcgattcac gttttggtgc gaagtctatt tccactatcg cagaatcaaa
    15481 acgttttccg ctgcacgaaa tgcgcgacga tgtcgcattc cagattatca atgacgaatt
    15541 atatcttgat ggcaacgctc gtcagaacct ggccactttc tgccagacct gggacgacga
    15601 aaatgtccac aaattgatgg atttatccat taa
//
LOCUS       DAJTSL010000091        15629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTSL010000091 DAJTSL010000000
VERSION     DAJTSL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.091"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(124..1407)
                     /gene="gltA"
                     /locus_tag="OO433_002573"
     CDS             complement(124..1407)
                     /gene="gltA"
                     /locus_tag="OO433_002573"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6050999.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1613..1720)
                     /locus_tag="OO433_002574"
     CDS             complement(1613..1720)
                     /locus_tag="OO433_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051000.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2101..2505
                     /gene="sdhC"
                     /locus_tag="OO433_002575"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2101..2505
                     /gene="sdhC"
                     /locus_tag="OO433_002575"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6051001.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2499..2846
                     /gene="sdhD"
                     /locus_tag="OO433_002576"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2499..2846
                     /gene="sdhD"
                     /locus_tag="OO433_002576"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6051002.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2846..4612
                     /gene="sdhA"
                     /locus_tag="OO433_002577"
     CDS             2846..4612
                     /gene="sdhA"
                     /locus_tag="OO433_002577"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6051003.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4628..5344
                     /gene="sdhB"
                     /locus_tag="OO433_002578"
     CDS             4628..5344
                     /gene="sdhB"
                     /locus_tag="OO433_002578"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6051004.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5645..8446
                     /gene="sucA"
                     /locus_tag="OO433_002579"
     CDS             5645..8446
                     /gene="sucA"
                     /locus_tag="OO433_002579"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6051005.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8461..9678
                     /gene="odhB"
                     /locus_tag="OO433_002580"
     CDS             8461..9678
                     /gene="odhB"
                     /locus_tag="OO433_002580"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6051006.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9954..11120
                     /gene="sucC"
                     /locus_tag="OO433_002581"
     CDS             9954..11120
                     /gene="sucC"
                     /locus_tag="OO433_002581"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6051007.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPQLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQLDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKVVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11120..11989
                     /gene="sucD"
                     /locus_tag="OO433_002582"
     CDS             11120..11989
                     /gene="sucD"
                     /locus_tag="OO433_002582"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6051008.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12093..12815)
                     /gene="mngR"
                     /locus_tag="OO433_002583"
     CDS             complement(12093..12815)
                     /gene="mngR"
                     /locus_tag="OO433_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6051009.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12984..14900
                     /gene="mngA"
                     /locus_tag="OO433_002584"
     CDS             12984..14900
                     /gene="mngA"
                     /locus_tag="OO433_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6051010.1"
                     /translation="MNLTTLTHRDALRLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTMHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVPACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAIFGFMCSFDLGGPVNKAAYAFCLGAMVNGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14918..>15629
                     /locus_tag="OO433_002585"
     CDS             14918..>15629
                     /locus_tag="OO433_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6051011.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPTDENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKL"
BASE COUNT         3726 a         3939 c         4272 g         3692 t
ORIGIN      
        1 ttttagaata tatgaatgtc cgatagtgga ttttattttt acaatttttt gtgtttaggc
       61 atataaaaat taacccgcca tttgaacggc gggttaaaat atttacaact tagcaatcaa
      121 ccattaacgc ttgatatcgc ttttaaagtc gcgtttttca tatcctgtat acagctgacg
      181 cggacgggca atcttcatac cgtcactgtg catttcgctc cagtgggcga tccagccaac
      241 ggtacgtgcc attgcgaaaa tgacggtgaa catggaagac ggaataccca tcgctttcag
      301 gatgatacca gagtagaaat cgacgttcgg gtacagtttc ttctcgataa agtacgggtc
      361 gttcagcgcg atgttttcca gctccatagc cacttccagc aggtcatctt tggtgcccag
      421 ctctttcaga acttcatggc aggtttcacg cattacggtg gcgcgcgggt cgtaattttt
      481 gtacacgcgg tgaccgaagc ccatcaggcg gaaagaatca tttttatctt tcgcacgacg
      541 aacaaattcc ggaatgtgtt taacggagct aatttcttcc agcattttca gcgccgcttc
      601 gttagcacca ccgtgcgcag gtccccacag tgaagcaata cctgctgcga tacaggcaaa
      661 cgggttcgca cccgaagagc cagcggtacg cacggtggag gtagaggcgt tctgttcatg
      721 gtcagcgtgc aggatcagaa tacggtccat agcacgttcc agaatcggat taacttcata
      781 cggttcgcac ggcgtggaga acatcatatt caggaagtta ccggcatagg agagatcgtt
      841 gcgcggataa acaaatggct gaccaatgga atacttgtaa cacatcgcgg ccatggtcgg
      901 cattttcgac agcaggcgga acgcggcaat ttcacgatga cgaggattgt taacatccag
      961 cgagtcgtga tagaacgccg ccagcgcgcc ggtaatacca cacatgactg ccattggatg
     1021 tgagtcgcga cggaaagcgt ggaacagacg ggtaatctgc tcgtggatca tggtatgacg
     1081 ggtcaccgta gttttaaatt cgtcatactg ttcctgagtc ggtttttcac cattcagcag
     1141 gatgtaacaa acttccaggt agttagaatc ggtcgccagc tgatcgatcg ggaaaccgcg
     1201 gtgcagcaaa ataccttcat caccatcaat aaaagtaatt ttagattcgc aggatgcggt
     1261 tgaagtgaag cctgggtcaa aggtgaacac accttttgaa ccgagagtac ggatatcaat
     1321 aacatcttga cccagcgtgc ctttcagcac atccagttca acagctgtgt ccccgttgag
     1381 ggtgagtttt gcttttgtat cagccattta aggtctcctt agcgccttat tgcgtaagac
     1441 tgccggaact taaatttgcc ttcgcacatc aacctggctt tacccgtttg ttatttggct
     1501 cgccgctctg tgaaagaggg gaaaacctgg gtacagagct ctgggcgctt gcaggtaaag
     1561 gatccattga tgacgaataa atggcgaatc aagtacttag caatccgaat tattaaactt
     1621 gtctaccact aataactgtc ccgaatgaat tggtcaatac ttccacactg ttacataagt
     1681 taatcttagg tgaaataccg acttcataac ttttacgcat tatatgcttt tcctggtaat
     1741 gtttgtaaca actttgttga gtgattgtca aattagatga ttaaaaatta aataaatgtt
     1801 gttgtcgtga cctggatcac tgttcaggat aaaacccgac aaactatatg taggttaatt
     1861 gtaatgattt tgtgaacagc ctatactgcc gctaggtctc cggaacaccc tgcaatcccg
     1921 agccacccag cgttgtaacg tgtcgttttc gcatctggaa gcagtgtttt gcatgacgcg
     1981 cagttataga aaggacgctg tctgacccgc aagcagaccg gaggaaggaa atcccgacgt
     2041 ctccaggtaa cagaaagtta acctctgtgc ccgtagtccc cagggaataa taagaacagc
     2101 atgtgggcgt tattcatgat aagaaatgtg aaaaaacaaa gacctgttaa tctggaccta
     2161 cagaccatcc ggttccccat cacggcgata gcgtccattc tccatcgcgt ttccggtgtg
     2221 atcacctttg ttgcagtggg catcctgctg tggcttctgg gtaccagcct ctcttcccct
     2281 gaaggtttcg agcaagcttc cgcgattatg ggcagcttct tcgtcaaatt tatcatgtgg
     2341 ggcatcctta ccgctctggc gtatcacgtc gtcgtaggta ttcgccacat gatgatggat
     2401 tttggctatc tggaagaaac attcgaagcg ggtaaacgct ccgccaaaat ctcctttgtt
     2461 attactgtcg tgctttcact tctcgcagga gtcctcgtat ggtaagcaac gcctccgcat
     2521 taggacgcaa tggcgtacat gatttcatcc tcgttcgcgc taccgctatc gtcctgacgc
     2581 tctacatcat ttatatggtc ggttttttcg ccaccagtgg cgaactgaca tatgaagtct
     2641 ggatcggttt cttcgcctct gcgttcacca aagtgttcac cctgctggcg ctgttttcta
     2701 tcttgattca tgcctggatc ggcatgtggc aggtgttgac cgactacgtt aaaccgctgg
     2761 cattgcgcct gatgctgcaa ctggtgattg tcgttgcact ggtggtttac gtgatttatg
     2821 gattcgttgt ggtgtggggt gtgtgatgaa attgccagtc agagaatttg atgcagttgt
     2881 gattggtgcc ggtggcgcag gtatgcgcgc ggcgctgcaa atttcccaga gcggccagac
     2941 ctgtgcgctg ctctctaaag tcttcccgac ccgttcccat accgtttctg cgcaaggcgg
     3001 cattaccgtt gcgctgggta atacccatga agataactgg gaatggcata tgtacgacac
     3061 cgtaaaaggg tcggactata tcggtgacca ggacgcgatt gaatatatgt gtaaaaccgg
     3121 gccggaagcg attctggaac tcgaacacat gggcctgccg ttctcgcgtc ttgatgatgg
     3181 tcgtatctat caacgtccgt ttggcggtca gtcgaaaaac ttcggcggcg agcaggcggc
     3241 acgcactgcg gcggcggctg accgtaccgg tcacgcactg ttgcacacgc tttatcagca
     3301 gaacctgaaa aaccacacca ccattttctc cgagtggtat gcgctggatc tggtgaagaa
     3361 ccaggatggc gcggtggtgg gttgtactgc actgtgcatc gaaactggtg aagtggttta
     3421 tttcaaagcc cgcgctaccg tgctggcgac tggcggggca gggcgtattt atcagtccac
     3481 cactaacgcc cacattaaca ctggcgacgg cgtcggcatg gctatccgcg caggcgtacc
     3541 ggtgcaggat atggaaatgt ggcagttcca cccgaccggt attgccggtg cgggcgtact
     3601 ggtcaccgaa ggttgccgtg gtgaaggcgg ttatctgctg aacaaacatg gcgaacgctt
     3661 tatggagcgt tatgcgccga acgccaaaga cctggcgggc cgtgatgtgg tggcgcgttc
     3721 catcatgatc gaaatccgtg aaggtcgcgg ctgtgatggt ccgtgggggc cacacgcgaa
     3781 actgaaactc gaccacctgg gtaaagaagt tctcgaatcc cgtctgccgg gcatcctgga
     3841 actctcccgt accttcgctc acgtcgatcc ggtgaaagag ccgatcccgg ttatcccaac
     3901 ctgtcattac atgatgggcg gtattccgac caaagtgacc ggtcaggcgc tgactgtgaa
     3961 tgagaaaggc gaagatgtgg ttgttccggg actgtttgcc gttggtgaaa tcgcttgtgt
     4021 atcggtacac ggcgctaacc gtctgggcgg caactcgctg ctggacctgg tggtctttgg
     4081 tcgtgcggca ggtctgcatc tgcaagagtc tatcgccgag cagggcgcat tgcgcgatgc
     4141 cagcgagtct gatgttgaag catctctgga tcgtctgaac cgctggaaca ataatcgtaa
     4201 cggtgaagat ccggtggcga ttcgtaaagc gctgcaagaa tgtatgcagc ataacttctc
     4261 ggtcttccgt gaaggtgatg cgatggcgaa agggcttgag cagttgaaag ttatccgcga
     4321 gcgtctgaaa aatgcccgtc tggatgacac ttccagcgag ttcaacaccc agcgcgttga
     4381 gtgcctggaa ctggataacc tgatggaaac ggcgtatgca acggctgttt ctgccaactt
     4441 ccgtaccgaa agccgtggcg cgcatagccg cttcgacttc ccggatcgtg atgatgaaaa
     4501 ctggctgtgc cactccctgt atctgccaga gtcggaatcc atgacgcgcc gaagcgtcaa
     4561 catggaaccg aaactgcgcc cggcattccc gccgaagatt cgtacttact aatgcggaga
     4621 caggaaaatg agactcgagt tttcaattta tcgctataac ccggatgttg atgatgctcc
     4681 gcgtatgcag gattacaccc tggaagcgga agaaggtcgc gacatgatgc tgctggatgc
     4741 gcttatccag ctgaaagaga aagatcccag cctgtcgttc cgccgctcct gtcgtgaagg
     4801 tgtgtgcggt tccgacggtc tgaacatgaa cggcaagaat ggtctggcct gtattacccc
     4861 gatttcggca ctcaaccagc cgggcaagaa gattgtgatt cgcccgctgc caggtttacc
     4921 ggtgatccgc gatttggtgg tagacatggg acaattctat gcgcaatatg agaaaattaa
     4981 gccttacctg ttgaataatg gacaaaatcc gccagctcgc gagcatttac agatgccaga
     5041 gcagcgcgaa aaactcgacg ggctgtatga atgtattctc tgcgcatgtt gttcaacctc
     5101 ttgtccgtct ttctggtgga atcccgataa gtttatcggc ccggcaggct tgttagcggc
     5161 atatcgtttc ctgatcgata gccgtgatac cgagactgac agccgcctcg acggtttgag
     5221 cgatgcattc agcgtattcc gctgtcacag catcatgaac tgcgtcagtg tatgtccgaa
     5281 ggggctgaac ccgacgcgcg ccatcggcca tatcaagtcg atgttgttgc aacgtaatgc
     5341 gtaaaccgta ggcctgataa gacgcgcaag cgtcgcatca ggcaaccagt gccggatgcg
     5401 gcgtgaacgc cttatccggc ctacaagtca ttacccgtag gcctgataag cgcagcgcat
     5461 caggcgtaac aaagaaatgc aggaaatctt taaaaactgc ccctgacact aagacagttt
     5521 ttaaaggttc cttcgcgagc cactacgtag acaagagctc gcaagtgaac cccggcacgc
     5581 acatcactgt gcgtggtagt atccacggcg aagtaagcat aaaaaagatg cttaagggat
     5641 cacgatgcag aacagcgctt tgaaagcctg gttggactct tcttacctct ctggcgcaaa
     5701 ccagagctgg atagaacagc tctatgaaga cttcttaacc gatcctgact cggttgacgc
     5761 taactggcgt tcgacgttcc agcagttacc tggtacggga gtcaaaccgg atcaattcca
     5821 ctctcaaacg cgtgaatatt tccgccgcct ggcgaaagac gcttcacgtt actcttcaac
     5881 gatctccgac cctgacacca atgtgaagca ggttaaagtc ctgcagctca ttaacgcata
     5941 ccgcttccgt ggtcaccagc atgcgaatct cgatccgctg ggactgtggc agcaagataa
     6001 agtggccgat ctggatccgt ctttccacga tctgaccgaa gcagacttcc aggaaacctt
     6061 caacgtcggt tcatttgcca gcggcaaaga aaccatgaaa ctcggcgagc tgctggaagc
     6121 cctcaagcaa acctactgcg gcccgattgg tgccgagtat atgcacatca ccagcaccga
     6181 agaaaaacgc tggatccaac agcgtatcga gtctggtcgc gcgactttca atagcgaaga
     6241 gaaaaaacgc ttcttaagcg aactgaccgc cgctgaaggc cttgaacgtt acctcggcgc
     6301 aaaattccct ggcgcaaaac gcttctcgct ggaaggcggt gacgcgttaa tcccgatgct
     6361 taaagagatg atccgccacg ctggcaacag cggcacccgc gaagtggttc tcgggatggc
     6421 gcaccgtggt cgtctgaacg tgctggtgaa cgtgctgggt aaaaaaccgc aagacttgtt
     6481 cgacgagttc gccggtaaac ataaagaaca cctcggcacg ggtgacgtga aataccacat
     6541 gggcttctcg tctgacttcc agaccgatgg cggcctggtg cacctggcgc tggcgtttaa
     6601 cccgtctcac cttgagattg taagcccggt agttatcggt tctgttcgtg cccgtctgga
     6661 cagacttgat gagccgagca gcaacaaagt gctgccaatc accatccacg gtgacgccgc
     6721 agtgaccggg cagggcgtgg ttcaggaaac cctgaacatg tcgaaagcgc gtggttatga
     6781 agttggcggt acggtacgta tcgttatcaa caaccaggtt ggtttcacca cctctaatcc
     6841 gctggatgcc cgttctacgc cgtactgtac tgatatcggt aagatggttc aggccccgat
     6901 tttccacgtt aacgcggacg atccggaagc cgttgccttt gtgacccgtc tggcgctcga
     6961 tttccgtaac acctttaaac gtgatgtctt catcgacctg gtgtgctacc gccgtcacgg
     7021 ccacaacgaa gccgacgagc cgagcgcaac ccagccgctg atgtatcaga aaatcaaaaa
     7081 acatccgaca ccgcgcaaaa tctacgctga caagctggag caggaaaaag tggcgacgct
     7141 ggaagatgcc accgagatgg ttaacctgta ccgcgatgcg ctggatgctg gcgattgcgt
     7201 agtggcagag tggcgtccga tgaacatgca ctctttcacc tggtcgccgt acctcaacca
     7261 cgaatgggac gaagagtacc cgaacaaagt tgagatgaag cgcctgcagg agctggcgaa
     7321 acgcatcagc acggtgccgg aagcagttga aatgcagtct cgcgttgcca agatttatgg
     7381 cgatcgccag gcgatggctg ccggtgagaa actgttcgac tggggcggtg cggaaaacct
     7441 cgcttacgcc acgctggttg acgaaggcat tccggttcgc ctgtcgggtg aagactccgg
     7501 tcgcggtacc ttcttccacc gccacgcggt gatccacaac cagtctaacg gttccactta
     7561 cacgccgctg caacacatcc ataacgggca gggcgcgttc cgcgtctggg actccgtact
     7621 gtctgaagaa gcagtgctgg cgtttgaata cggttatgcc accgcagaac cacgcactct
     7681 gaccatctgg gaagcgcagt tcggtgactt cgctaacggt gctcaggtgg ttatcgacca
     7741 gttcatctcc tctggcgaac agaaatgggg ccggatgtgt ggtctggtga tgttgctgcc
     7801 gcacggttat gaagggcagg ggccggagca ctcctccgcg cgtctggaac gttatctgca
     7861 actttgtgct gagcaaaaca tgcaggtgtg cgtaccgtct accccggcac aggtttacca
     7921 catgctgcgt cgtcaggcgc tgcgcgggat gcgtcgtccg ctggtcgtga tgtcgccgaa
     7981 atccctgctg cgtcatccgc tggcggtttc cagcctcgaa gaactggcga acggcacctt
     8041 cctgccagcc atcggtgaaa tcgacgagct tgatccgaag ggcgtgaagc gcgtagtgat
     8101 gtgttctggt aaggtttatt acgacctgct ggaacagcgt cgtaagaaca atcaacacga
     8161 tgtcgccatt gtgcgtatcg agcaactcta cccgttcccg cataaagcga tgcaggaagt
     8221 gttgcagcag tttgctcacg tcaaggattt tgtctggtgc caggaagagc cgctcaacca
     8281 gggcgcatgg tactgcagcc agcatcattt ccgtgaagtg attccgtttg gggcttctct
     8341 gcgttatgca ggccgcccgg cctccgcctc tccggcggta gggtatatgt ccgttcacca
     8401 gaaacagcaa caagatctgg ttaatgacgc gctgaacgtc gaataaataa aggatacaca
     8461 atgagtagcg tagatattct ggtccctgac ctgcctgaat ccgtagccga tgccaccgtc
     8521 gcaacctggc ataaaaaacc cggcgacgca gtcgtacgtg atgaagtgct ggtagaaatc
     8581 gaaactgaca aagtggtact ggaagtaccg gcatcagcag acggcattct ggatgcggtt
     8641 ctggaagatg aaggtacaac cgtaacgtct cgtcagatcc ttggtcgcct gcgtgaaggc
     8701 aacagcgccg gtaaagaaac cagcgccaaa tctgaagaga aagcgtccac tccggcgcaa
     8761 cgccagcagg cgtctctgga agagcaaaac aacgatgcgt taagcccggc gatccgtcgc
     8821 ttgctggctg aacataacct cgacgccagc gctattaaag gcaccggcgt gggtggtcgt
     8881 ctgacccgtg aagatgtgga aaaacatctg gcgaaatccc cggcgaaaga gtctgcaccg
     8941 gcagcggctg ctccggcggc gcaaccggct ctggctgcac gtagtgaaaa acgtgtcccg
     9001 atgactcgcc tgcgtaagcg tgtggcagag cgtctgctgg aagcgaaaaa ctccaccgcc
     9061 atgctgacca cgttcaacga agtcaacatg aagccgatta tggatctgcg taagcagtac
     9121 ggtgaagcgt ttgaaaaacg ccacggcatc cgtctgggct ttatgtcctt ctacgtgaaa
     9181 gcggtggttg aagccctgaa acgttacccg gaagtgaatg cttctatcga cggcgatgac
     9241 gtggtttacc acaactattt cgacgtcagc atggcggttt ctacgccgcg cggcctagtg
     9301 acgccggttc tgcgtgatgt cgataccctc ggcatggcag acatcgagaa gaaaatcaaa
     9361 gagctggcag tcaaaggccg tgatggcaag ctgaccgttg aagatctgac cggtggtaac
     9421 ttcaccatca ccaacggtgg tgtgttcggt tccctgatgt ctacgccgat catcaacccg
     9481 ccgcagagcg caattctggg tatgcacgct atcaaagatc gtccgatggc ggtgaatggt
     9541 caggttgaga tcctgccgat gatgtacctg gcgctgtcct acgatcaccg tctgatcgat
     9601 ggtcgcgaat ccgtgggctt cctggtaacg atcaaagagt tgctggaaga tccgacgcgt
     9661 ctgctgctgg acgtgtagta gtttaagttt cacctgcact gtagacagga taaggcatta
     9721 tcgccttctc cggcaattga agcctgatgc gacgcttaac gcgtcttatc aggcctacgg
     9781 gaagcaccaa tgtaggtcgg ataaggcgct tgcgccgcat ccgacaagcg atgcctgatg
     9841 cgacacttac gcgtcttatc aggcctacag gtgaccgaca atgcccggag gcgatacgaa
     9901 atattcggtc tacggtttaa aagataacga ttgctgaagg atggacagaa cacatgaact
     9961 tacatgaata tcaggcaaaa caactttttg cccgctatgg cttaccagca ccggtgggtt
    10021 atgcctgtac tactccgcgc gaagcagaag aagccgcttc aaaaatcggt gccggtccgt
    10081 gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa agcgggcggt gtgaaagttg
    10141 taaacagcaa agaagacatc cgtgcttttg cagaaaattg gctcggcaag cgtctggtaa
    10201 cgtatcaaac agatgccaat ggccaaccgg ttaaccagat tctggttgaa gcagcgaccg
    10261 atatcgctaa agagctgtat ctcggtgccg ttgtagaccg tagttcccgt cgtgtggtct
    10321 ttatggcctc cactgaaggc ggcgtggaaa tcgaaaaagt ggcggaagaa actccgcagc
    10381 tgatccataa agttgcgctt gatccgctga caggcccgat gccgtatcag ggacgcgagc
    10441 tggcgttcaa actgggtctg gaaggtaaac tggttcagca gttcaccaaa atcttcatgg
    10501 gtctggcgac cattttcctg gagcgcgacc tggcgttgat cgaaatcaac ccgctggtca
    10561 tcaccaaaca gggtgatctg atttgcctcg acggcaaact gggcgctgat ggcaacgcac
    10621 tgttccgcca gcttgatctg cgcgaaatgc gtgaccagtc gcaggaagat ccgcgtgaag
    10681 cacaggctgc acagtgggaa ctgaactacg ttgcgctgga cggtaacatc ggttgtatgg
    10741 ttaacggcgc aggtctggcg atgggtacga tggacatcgt taaactgcac ggcggcgaac
    10801 cggctaactt ccttgacgtt ggcggcggtg caaccaaaga acgtgtaacc gaagcgttca
    10861 aaatcatcct ctctgacgac aaagtgaaag tcgttctggt taacatcttc ggcggtatcg
    10921 ttcgttgcga cctgatcgct gacggtatca tcggcgcggt agcagaagtg ggtgttaacg
    10981 taccggtcgt ggtacgtctg gaaggtaaca acgccgaact cggcgcgaag aaactggctg
    11041 acagcggcct gaatattatt gcagcaaaag gtctgacgga tgcagctcag caggttgttg
    11101 ccgcagtgga ggggaaataa tgtccatttt aattgataaa aacaccaagg ttatctgcca
    11161 gggctttact ggtagccagg ggactttcca ctcagaacag gccattgcat acggcactaa
    11221 aatggttggc ggcgtaaccc caggtaaagg cggtactacc cacctcggcc tgccggtgtt
    11281 caacaccgtg cgtgaagctg ttgctgctac tggcgctacc gcttctgtta tctacgtacc
    11341 agcaccgttc tgcaaagact ccattctgga agctattgat gcaggcatca aactgattat
    11401 caccatcact gaaggcatcc cgacgctgga tatgctgact gtgaaagtga aactggacga
    11461 agccggcgtt cgtatgatcg gcccgaactg cccgggcgtt atcactccgg gtgaatgcaa
    11521 aatcggtatc cagccgggtc acattcacaa accgggtaaa gtgggtatcg tttcccgttc
    11581 cggtacactg acctatgaag cggttaaaca gaccacggat tacggtttcg gtcagtcgac
    11641 ctgtgttggt atcggcggtg acccgatccc aggctctaac tttatcgaca tcctcgaaat
    11701 gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc ggtgagatcg gcggtagcgc
    11761 tgaagaagaa gcggcagcgt acatcaaaga gcacgttacc aagccagttg taggttacat
    11821 cgctggtgtg actgcgccga aaggcaaacg tatgggccac gcgggtgcga tcattgccgg
    11881 tgggaaaggg actgcggatg agaaattcgc tgctctggaa gccgcaggcg tgaaaaccgt
    11941 tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt ctgaaataaa tatctgtaat
    12001 aagaaatagc cctcgccgct tccctctaca ggaatggcga agggctgtcg gtttcgacat
    12061 ggttggccat cgtatgatgg ccttttttgt gcttatcgcg atgattttcg ctgcgctatc
    12121 agggtaaatt tatagtcatc ggtattaaaa gcgttgcggc tatattcaaa cacccgacca
    12181 tcaactaaat atccacgcga tactttttca agaatcggct ttgtctggct gatattaagc
    12241 agacggctca tctcttcggt tggcatcaga ggaatgattt cctgttcgct acgatcgata
    12301 accatcttct tcacttcttc aataaagtga tatttcgaat tttccatgac ctgccaggtg
    12361 agatccggga acaacgcaag cggcatccag gtttcttcca gcgccattgg cttttgcttg
    12421 cgatagcgca cgcgcttcac atgccacaca cgatcctgcg cggtgatttg tagctgttgc
    12481 tgaagaaaat cgtcagccgg aatcacttca aatatcagga cttcactgtg tgtatcgacg
    12541 tgacggtccg acagtttttc atcaaaactg gttaactgaa aaatatcgta attgacccgc
    12601 tcttctttga cgtaagtccc gctgccctga atgctttcga ggatctgctg ctcgactagc
    12661 tggcgcaaag cctgacgcac cgtaacccgg ctgacgccaa actctgtttg tagcgctgat
    12721 tcagtgggta acgcatcgcc aggtttaagc tcgccacgcg caatttgttc acgaatgcga
    12781 tcggcaatct gccggtataa gggcttgtgt cccattttta gtatctcatt aatacgaatt
    12841 tagccattat gcccgataaa ttcatcctgt aaataatgca aatacaatac aaataatttc
    12901 aatcaagtga aattgatcat ataatggtat tgttttatcg ggcacactgg cgcgactata
    12961 aaaacgatca agtgaggatc atgatgaatc tgacgactct gacccaccgc gatgcgttgc
    13021 gtctgaatgc gcgctttacc agccgtgaag aggccatcca cgcgttgact caacgtcttg
    13081 ctgctctggg gaaaatttcc agtactgagc aatttctgga agaagtgtat cgccgtgaaa
    13141 gccttggccc gaccgcctta ggtgaagggt tggctgtgcc gcatggcaaa actgctgcgg
    13201 taaaagaagc ggcgtttgcg gtcgccacac tcagcgagcc gcttcagtgg gaaggcgttg
    13261 atggcccgga agcagttgat ttagtggtgc tgctggcgat tccccccaat gaagcgggta
    13321 ctatgcatat gcaactgctg acagcgctga ccacgcgcct tgcggatgat gagattcggg
    13381 cgcgtataca gtcggcgacg acgcctgatg agttgctctc ggcgctggat gacaagggag
    13441 gcacgcaacc ttctgcctct ttttccaacg cgccaactat cgtctgcgta ccggcctgtc
    13501 cggcgggtat tgctcacacc tatatggctg cggaatatct ggaaaaagcc ggacgcaaac
    13561 tcggcgtaaa tgtttacgtt gaaaaacaag gcgctaacgg cattgaaggg cgtttaacgg
    13621 cggatcaact caatagtgca accgcctgta tttttgcggc tgaagtcgcc atcaaggaga
    13681 gtgagcgttt taatggcatt cccgcgcttt cagtgcctgt tgccgagccg attcgccatg
    13741 cagaagcgtt gatccaacaa gcgcttaccc tcaagcgtag cgatgagacg cgtaccgtac
    13801 agcaagatac gcaaccggtg aaaagtgtca aaacggagct gaaacaggca ctgttgagcg
    13861 gaatctcttt tgccgtaccg ttgattgtcg cggggggcac ggtgctggcg gtcgcggtat
    13921 tactgtcgca aatcttcggg ctacaagatc tgtttaatga agaaaactcc tggctgtgga
    13981 tgtaccgcaa gctgggcggc gggctgctcg gaattttgat ggtaccggtg ctcgcggcct
    14041 atactgccta ttctctggca gataaaccgg cgttagcgcc aggctttgcg gctggacttg
    14101 ccgccaacat gatcggctcc gggtttctcg gcgcggtcgt tggcggattg atagccggtt
    14161 acttgatgcg ctgggtgaaa aatcacttgc gtcttagcag taaattcaat ggattcctga
    14221 ctttttatct ctacccggtg ctcggtacgt tgggagcggg cagtctgatg ctgtttgtgg
    14281 tgggagaacc tgtcgcatgg atcaataact cgcttaccgc ctggctgaac ggtctgtcag
    14341 gaagtaacgc gctgttgctg ggtgccattt tcggttttat gtgttccttt gatcttggag
    14401 ggccagtgaa caaagccgct tatgcattct gcctgggcgc aatggtgaac ggcgtttacg
    14461 gcccgtatgc cattttcgcc tccgtcaaaa tggtttcggc atttaccgta accgcttcca
    14521 cgatgctcgc accgcgcctg tttaaagagt ttgaaattga gaccgggaaa tccacctggc
    14581 tgttagggct ggcaggtatt accgaagggg cgatcccgat ggcgattgaa gatccgctgc
    14641 gggttattgg ttcgtttgtg ctgggctcta tggtaacggg cgctattgtc ggtgcgatga
    14701 atatcggcct ttcgacaccc ggtgccggca ttttctcgct ctttttactt catgataatg
    14761 gcgcgggcgg tgttatggcg gcaattggct ggtttggtgc ggcattggtg ggggctgcaa
    14821 tctcgactgc aattctcctg atctggcggc gtcacgcggt taagcatggc aactatctga
    14881 ctgatggcgt aatgccataa acaaaaagga aacgacgatg aaagcggtat ctcgtgttca
    14941 catcaccccg catatgcact gggatcgaga gtggtatttc accaccgaag agtcacgtat
    15001 tctgctggtc aataatatgg aagagatcct gtgccgactg gaacaggaca acgaatacaa
    15061 atattacgta ctcgacgggc aaacggcgat cctcgaagat tatttcgcgg tgaaaccgga
    15121 aaacaaagac cgtgtgaaga aacaggtaga agccggcaag ttgattatcg gcccctggta
    15181 tacccagacc gataccacga ttgtttctgc ggaatccatc gtccgtaatc tgatgtacgg
    15241 aatgcgtgac tgcctcgcgt ttggcgagcc gatgaaaata ggttatttac cagattcctt
    15301 tggcatgtcc gggcaactgc cgcatatcta caatggattt ggcattaccc gcaccatgtt
    15361 ctggcgcgga tgttcggagc gccacggtac tgataaaacc gagtttttgt ggcaaagcag
    15421 tgacggtagc gaagtgacgg cgcaggtgct gccgctgggc tacgccatag gtaagtactt
    15481 acctaccgac gaaaacggat tacgtaaacg cctcgacagt tattttgacg tgctggaaaa
    15541 agcgtctgta accaaagaga ttttgctgcc gaatggtcat gaccagatgc cattgcagca
    15601 aaatatcttc gaagtgatgg ataagctac
//
LOCUS       DAJTSL010000092        15099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTSL010000092 DAJTSL010000000
VERSION     DAJTSL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.092"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..607
                     /locus_tag="OO433_002586"
                     /pseudo
     CDS             1..607
                     /locus_tag="OO433_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490532.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            821..1969
                     /locus_tag="OO433_002587"
     CDS             821..1969
                     /locus_tag="OO433_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6051012.1"
                     /translation="MFEIDSLLLITSVIILSLLAVKLFDEISPIQLVDHGRNNQIDGM
                     RGFLAIFVLIHHAAIWHGYLLTGVWETPSSNLLTNLGQVGVSFFFMITGYLFFSKIRS
                     SDQDWVRLYISRFLRLTPMFIVSLCLVLLVIGFKSRWSVHVSPASLTVSLMRWAPFTA
                     LGMPNINGVKDSFTINAAVTWTLVYEWFFYFSLPVIAALFKRRVSIHMIIISVLVLVI
                     FLCYPSKKIHVISFLFGLLASYSNNYVHRVAKAKITPIMIIALLSYEMIYFPTTYAPL
                     PLIICGVCFILIASGCDFYGILRLNLTRKLGETTYSIYLLHGVFLYCVMTWVIPSNYS
                     NYFFIMLISITAFCVTLLSCITFKLIELPFINITKQTAMKIRGIIKNT"
     gene            complement(2249..2860)
                     /locus_tag="OO433_002588"
     CDS             complement(2249..2860)
                     /locus_tag="OO433_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6051013.1"
                     /translation="MNNAELNSELIATMAGEITVYNFDVMSREYISASTEYLAIGVGI
                     PAYSCLDAPGAYKAGYAICRSADFNSWEYVPDHRGEIVFSTETGESKEITAPGDYPDN
                     TTTIVPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDTAMASINLIQLKLQAG
                     RNLTQAETSRLNTVLDYIDAVTATDTSTAPDVIWPELLDAHNM"
     gene            complement(2860..3642)
                     /locus_tag="OO433_002589"
     CDS             complement(2860..3642)
                     /locus_tag="OO433_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR6051014.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEG
                     SALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDD
                     GRGVDNGRGLLTLQDGAIVSHNHYWGIWTSRTNDQTLESFTGTTILKQITPLSPSIDF
                     DNYPIPNPAITEGGVVAATTKPAGANETRPRNVAFNYIVRAA"
     gene            complement(3646..4230)
                     /locus_tag="OO433_002590"
     CDS             complement(3646..4230)
                     /locus_tag="OO433_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR6051015.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMQELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRFWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            complement(4221..5279)
                     /locus_tag="OO433_002591"
     CDS             complement(4221..5279)
                     /locus_tag="OO433_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6051016.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATVASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTTTDDAISSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NITIAKNELAVLGAISWT"
     gene            complement(5266..5691)
                     /locus_tag="OO433_002592"
     CDS             complement(5266..5691)
                     /locus_tag="OO433_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6051017.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLRRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(5691..6239)
                     /locus_tag="OO433_002593"
     CDS             complement(5691..6239)
                     /locus_tag="OO433_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR6051018.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(6239..7318)
                     /locus_tag="OO433_002594"
     CDS             complement(6239..7318)
                     /locus_tag="OO433_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR6051019.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARKRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(7315..8643)
                     /locus_tag="OO433_002595"
     CDS             complement(7315..8643)
                     /locus_tag="OO433_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR6051020.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQSTTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            complement(8704..10539)
                     /locus_tag="OO433_002596"
     CDS             complement(8704..10539)
                     /locus_tag="OO433_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6051021.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYQAVDPAQSNNASGATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            complement(10681..10950)
                     /locus_tag="OO433_002597"
     CDS             complement(10681..10950)
                     /locus_tag="OO433_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6051022.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(10950..11306)
                     /locus_tag="OO433_002598"
     CDS             complement(10950..11306)
                     /locus_tag="OO433_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6051023.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNKTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(11306..12802)
                     /locus_tag="OO433_002599"
                     /pseudo
     CDS             complement(11306..12802)
                     /locus_tag="OO433_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
     gene            complement(12786..12956)
                     /locus_tag="OO433_002600"
     CDS             complement(12786..12956)
                     /locus_tag="OO433_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6051024.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(12983..13525)
                     /locus_tag="OO433_002601"
     CDS             complement(12983..13525)
                     /locus_tag="OO433_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051025.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLEPGNGPDGDIEHHT"
     gene            complement(13522..14028)
                     /locus_tag="OO433_002602"
     CDS             complement(13522..14028)
                     /locus_tag="OO433_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051026.1"
                     /translation="MATPFFHVDVQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKKRAGFMTRIAPNQRNGKGNRMISGDFYP
                     AFLFFGVRGGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            complement(14003..14413)
                     /locus_tag="OO433_002603"
     CDS             complement(14003..14413)
                     /locus_tag="OO433_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6051027.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCDDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGSNGDSLFSR"
     gene            complement(14410..14733)
                     /locus_tag="OO433_002604"
     CDS             complement(14410..14733)
                     /locus_tag="OO433_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR6051028.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            complement(14736..14936)
                     /locus_tag="OO433_002605"
     CDS             complement(14736..14936)
                     /locus_tag="OO433_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051029.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            complement(14985..>15099)
                     /locus_tag="OO433_002606"
     CDS             complement(14985..>15099)
                     /locus_tag="OO433_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR6051030.1"
                     /translation="LRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLLKIAVA"
BASE COUNT         3486 a         4291 c         3628 g         3694 t
ORIGIN      
        1 agagcccgca ccaacgctga aaatcaaaga cccgcactat gatgaaaagc accttgctat
       61 tgagcaggcg ctgactcagg ggcagacgaa acatcctgtg ttttatcagg atgaagtcga
      121 catcgacctg aatacgaaaa tcggcgtgga ctggatgccc aaagggcaac agaaacgcat
      181 cgctacgccg ggacagaacc tgaagcatta tctggctggc gcactgcact caggtacggg
      241 acgggttcac taggccagtg gcagcagcaa aagttctgat ttatttatca gtctgttaga
      301 ggcattacgg cgtagatatc ggcgggcgaa aaccatcaca ctggtggcgg ataactacat
      361 catccataaa ggccgtaaag tggagcgatg gctggaagaa aacccgaagt tccggttgct
      421 gttcctgccg acgtattcac cgtggctgaa tccgatagag caactttggt tatcgttaca
      481 caagaccata acgcgaaatc atcagtgccg gtatatgtgg cagatactga aacaggtaac
      541 gcagtttatg aatgctgctt taccgtttcc gggtaatcaa cacggtatgg ccaaagtgga
      601 gcggtaatat gcgaagctat ttagattcag tttgaatctc agccctgaca ttcattcttt
      661 accgacgttc cattagccca aatctgagta tataacctca attcaactga ctaaatttaa
      721 cacaatatca atgacaaatg tctttaccat tggtacatac aattgcaaaa tcaccatctc
      781 taagcaataa gactacgcat attaaagtga gttatattaa atgtttgaaa ttgatagcct
      841 attattaata acatccgtga taatcttgtc attattggct gtaaaattat ttgatgaaat
      901 ttctcctata caacttgtcg atcatggaag aaataatcag atagatggaa tgagaggatt
      961 cttagccatc ttcgtactta ttcatcatgc cgctatttgg catggctact tattaaccgg
     1021 ggtatgggaa actccgtcat caaacttatt aacaaattta ggccaagttg gtgtctcatt
     1081 ctttttcatg atcaccggat atctattttt ttcgaagatt cgctcaagtg accaggattg
     1141 ggtaagacta tatatatcga gatttctgcg attaactcct atgtttattg taagtttatg
     1201 tttagttctt cttgttatag gttttaaatc aagatggagc gtacatgtct cccctgcctc
     1261 tcttactgtg tcactcatga gatgggcacc attcaccgca ttagggatgc caaatattaa
     1321 cggtgttaag gactcattca ctatcaatgc agctgtaaca tggaccctcg tttacgaatg
     1381 gtttttttat ttttcattac cggttatagc tgcactcttc aaaagaagag tcagcatcca
     1441 catgattata ataagtgttt tagttctagt gattttctta tgttacccat caaaaaaaat
     1501 acatgttatt tcatttttat ttggactgtt agcctcttat tcaaataatt atgtacatag
     1561 agttgctaaa gctaaaataa cgcctattat gataattgca ttactaagtt acgaaatgat
     1621 atattttcct acaacctacg ctccactccc tctcataatt tgcggtgttt gctttatact
     1681 tatcgcatca ggctgtgatt tttatggtat attgagatta aatttaacaa gaaaacttgg
     1741 tgaaacaaca tatagtattt atcttttaca tggagtattc ctttattgtg taatgacatg
     1801 ggtcatccct agtaattatt ctaattattt ttttattatg ttaataagca ttactgcttt
     1861 ttgcgtcaca cttttatcat gcataacatt taaattaatt gaactacctt tcattaatat
     1921 aacaaaacaa accgctatga aaatcagggg cataataaaa aacacttagt aatcaattgt
     1981 cacctaatca agaggtggtt tatagataac aataaagaac cttcgccatt tccggaggtt
     2041 cttttatatc cttatggtaa atataaatta taaatattat ttttatggtg ttccactatg
     2101 aacagggaag ctaaataatg attacaaaaa tatgtaactt caggcaatgc aggggctatg
     2161 ttattgcctt aacaccataa tgagtaatat tgtgtcccga ggataaaagt actgttaatt
     2221 cttattgatg cttcaatagc actcaaaatc acatattatg tgcatccaac aactcaggcc
     2281 agatgacatc cggcgcggtg ctggtatctg ttgccgtcac cgcgtcaatg taatccagca
     2341 ccgtgttaag tcggctggtt tctgcctgcg tcagattccg tccggcctgt aattttagtt
     2401 gaatcagatt gatggaagcc attgcagtat caatcagcga ctggcgttgt gcttctgctg
     2461 catcgactgc ggcgctatgt tgtgcctcgg tatccgtcac ccatttctca ccatcccatt
     2521 tatcgtatgg tgttaacggg acgatagtgg ttgtattatc agggtaatca cccggagctg
     2581 tgatttcttt tgattctcct gtttcggtgc taaagacgat ttcaccgcga tggtctggca
     2641 catattccca tgagttaaaa tctgcagaac ggcagattgc ataaccagct ttgtatgcgc
     2701 ctggagcatc taaacaggaa tatgccggaa tgccgacacc aatagcaaga tattcagttg
     2761 aagctgaaat atactcccga ctcatgacat caaagttata aacagtaatt tctcctgcca
     2821 tagtggcaat taattcactg tttaattctg cgttattcat tatgcagccc tcacaatata
     2881 gttaaaagcg acatttcgtg ggcgtgtttc attcgcacct gcaggtttag tcgttgccgc
     2941 aacaacacca ccctctgtaa tagccgggtt gggaattggg taattatcga agtcaatgga
     3001 cggagacagg ggcgtgattt gttttaaaat cgtggtgcct gtaaaacttt ccagagtctg
     3061 gtcgttagtt cgtgaagtcc agattcccca atagtggtta tgactgacaa tagcaccgtc
     3121 ctgaagcgtt aataatcccc ttccgttatc cactccacgc ccgtcatccc agccccgaat
     3181 aaactcaccg cgtaaatcag gtaatttatt tgtcggataa gcctttgcca gttccgggta
     3241 ttcttcagca gaaaaagctg ctccgttgca tttcagccag cctgttggcg gagtggctga
     3301 aggccacgga acaggcacac caacgggtaa tgctgagcct tctcccaaac caaggttttc
     3361 gagagctgtt ttcaccgtgc catccgattt gatatcgcca aacggattct tgcggcttaa
     3421 cagcagcgca cgaagcgcgg taagcagctg gtcatgccgc cccttctcca ggctggcacc
     3481 ggatgcctcc acaacgctgc aaagctcctc ctgcaacatg tcaaagtagt catcatccag
     3541 atcggtggca ggcgtgccgg tctgggggtt accacgggta aaaccgttct tacccgcgcc
     3601 gaacttatcc ttctgcgcgg ttttcgtgtc tatacgatgc atggattact ccggatattt
     3661 aaaaatcaca taggtatgcg acgggcagag tttgttaaga acacactcga caacggtgtc
     3721 cccccagaag cgcagcgcgg aatcacaggg atcgccacat gtcatccagg tggtgttggt
     3781 ggcggctggc atgttgacct gccagtaata ccgccattcc ggcgcattca ccgcgtcagt
     3841 acaggcagat gagcaggtga acgtgctttt gtcgtatcgc gtgatggtgg cgtctggtct
     3901 gcccaaggca gcaagctgtg caaggtaaaa atcctcattg atgccgcccg ccaggttaac
     3961 cttcgcatcc agccgttgct gacgctggcg aagggtctgc gtccctgccg gaatacattc
     4021 atccggcagg ccgcacagac gctcccagcg gtttatcagt tcggtggtgg tgcgcggatc
     4081 cagctcctgc atcagggcat ccgcacgctg atgaacacgg gttaatgacg gtgccgcacc
     4141 ggcaatcgcc ggatcgctgg ctgaccacgc cggaccgggc ggcaacagtg ctgacaacag
     4201 acggatgtaa tcatcgtttg tcacgtccat gaaatcgccc ccagaaccgc cagttcattt
     4261 ttcgcaatgg tgatattgtc cgccggtgca agcaactgat ggctgtattc cccgttcgca
     4321 ccggaaatcg cttcactgat acgtgacacc ttcagttctc cctgcggata accatcacgc
     4381 agcaggaacg aacgcaactc cgcggtgatg gcagcccgta tttccggtgt gtccggcgtc
     4441 acacggatat gaaaatccac cgtatgtgcc accggcctga acacatacaa atcagagcct
     4501 gccaccgggg ccagtggccc gatatgttgt cttgccgccg tttccgttga ttcttccgga
     4561 atgggattaa tcaggtcact gctggcaatc atcacaccga cagtccccgt tcccatccag
     4621 tgtcggtatg tccatgcgcg ggtaatgcca ggcacttctt tagcccagac gacatagtcc
     4681 ccgtcagccc cgccctgcgg cgtccagtaa taccgctcaa tgacgcgggc gcgccacgtt
     4741 tccagctctt cagtatcaaa tccgcctgtc agggtgtcag ccacaccgga agacggcaga
     4801 ccattcaccg gcgtgaccag gattaatgcc gtaccgtcgt cagcgttacc gaccgcacct
     4861 gcacttgagc aggcgatcgg cacgcgcagg acaccaccgg agctgattgc atcgtcagtt
     4921 gtcgtgtact gcaccaggtc atcgcgctga ataacactcc cggcagtcac cttcaggcca
     4981 tcgctgacac cttcccagcg catatacccg ctggcaaccg tggccccctt gcgcggacac
     5041 cgtttcatcg cagcatgtcg cgccagccag gactcatcgc acaggtcagg cagcatattc
     5101 attgccagat aatcgatgta accgtagacc gtatgcagcg ccgccgcata cacctttgcc
     5161 cgcacgtctt catccatgcg ccggagcgtg tcgctgacgt ccagcctggc gaataaatcg
     5221 ttacggagca tactgatatt ttctgccagc gtcgggcgct gaaattcact gtccgccatg
     5281 cgttatcgca ctccacagat catcaaaaga aatcattact ggtccatcac gacgcctgag
     5341 ggtgatactg ttacccagct cattaatccc ggtgcggcgg atatccagat caatacggga
     5401 caccacgccg tcatcagtca tccattgcag gcattcgcgg atatatcccc ttaccgtctg
     5461 caccagctga ttggtcagtt tgctgcgctg aagcaaccac agtcgggagc cgtaacggtc
     5521 attctgtacc gcaggccagg tatcccccca ccatcccatc gggacgtcgg cattgtcatc
     5581 aggctccgcc cgccgccagg tgaacaggga aatcaccacg gcgcgggtca gcggatccag
     5641 cggtgcgctg gcgcaggtgc gtttaccgtt caccgtcagc cacagttcca tcatgcctcc
     5701 atcgctttat caggtttgtc ggtgttactg ccctgaccgt tctctctgtg acgatgcccg
     5761 ttataggcaa gccgcatcgc tgacatggtg gtgccgccgg agtcgcacag gtctttcacc
     5821 tgtccggtca cttccagatc catttcaaaa cgtgcttcag gtgcattgcg aaacgtgatc
     5881 gttttccctg caccgtccac cacgatcccc tcccgggtca gcgtcacaga ctgcccctga
     5941 tcgtcataga cagccacctc acccgtttgc agccctttca ggcggtagcg ccggtccgac
     6001 accgtaacaa ccaccgcatg agaacggtcg ccatccggaa acaacaccac cgcttccgca
     6061 ccgctgtttg cccttgcggt aaaaccgtag ggttcaagat gttcaacccc ggctttgggt
     6121 tcaccggcaa tcagggacac atccacggtc tgacatttcg tggcggcact gatgcttttc
     6181 accactgccc gcccaatcag gccgagaagt tgtcgctgca tggcttcaat cgtcctcatc
     6241 agaacgggtc ctcctgtact ctggcttttt tctttttccg cgcgccgggg gcttcgggtt
     6301 caggcagata agcgtcaggc gggccgacac ggatttccgt cagggtgccg ttctggtcct
     6361 gagtaaacgt gacttccgag acaagcagtt cggtattgtc gaaaccacag accggatcga
     6421 agacaatcac ccgctggttg ggctgccaca gcgtaccgtt accctgtcgc cagccctgca
     6481 ccacataggt ggtttcatcc gtccgcgccg cccgttttcg ggcttcaaag tcagcacgcg
     6541 caatacagcc tgcccccgtg gcctgccctg tctgcctgat atacatcgga cggtaacggg
     6601 caataaatgc gtcctctgtg cgggcccgca gcgcggtggt ggtggcctca ccgaaatcat
     6661 cgtcgtttcc ggcacgctgc cccgccacct ggtaaacaga aaaccgctcc cggatactct
     6721 tctccgtatc acaggaaagg atgttttccc caagtaccag cgcggtatgt gcccgcgttg
     6781 agccaatacc gccaatcacc agcctgccgt gcgggtcgtc gtaagccagc gcctgctgct
     6841 gaccgagtat tttgttgatt acctcaatca ccgtttcacc gtgatcaggc tgaacatcag
     6901 gaataacacc cgacggcgca ccgctgttca ccacctcaat gccgaaaggc gcagcaagcg
     6961 cctgcgcaat ctgcaccagc gagcgtccgt taaactgtgt cggttcggct gcacagtcaa
     7021 tcaggtcagc cgtcagacta cgtccggcaa taccggtgct gaccgaacgg gcatcgtaac
     7081 gaacgggagt cgcctccacc cagccggtga tcaccagctc atcaccaatc agcacttcca
     7141 cttttgaacc gtttttaatg cgcggctgaa gcgtggtgat accctcatct cccggccatt
     7201 ggcgagtgat ctccacactg aaatcccgcg ccagccgttc aataccggca ccgatgcgca
     7261 ccgatgtcca gccattccac tcccggccat ttacccgtag cgtgacattg tcgttcattg
     7321 cactggcacc ttcagaggga tcaccggcac aaagccggga tgcgtaatgg cattacgccg
     7381 aataatgtcc gcgtcacgcg ccgcgttatc aaaccaggtc gccgccagca ccagcgcggg
     7441 taaaacctca tccggcgtgc gctgaatgat ccgtgcagac tgttcaaggc gcgtgttgat
     7501 atccgcattc agatctgctt tcacccggcg cagcgccaga aacagcgcat cactggttgt
     7561 acgggacaac tccttatcaa ttgccgtatt cagtgtgtcg cgaatgtcgg tcagttcttc
     7621 ccacgttggc aggtcaaccg tgtttttcac cgccggtgca ttgttcagtg ccggatgcgt
     7681 gacagaaggc cagccggtgc tctgcgcggg tgttgttgac tgccctactg tggcattctg
     7741 catcaccgcg gaagttgtgg gcgcaggcaa tcgtgtgacg gcatacgccg cttcgctgat
     7801 tgcggtcgta cgaagggtgc tggcaaccac gttacgctgc tgcgtcgccg tggcggtagt
     7861 tttactgtcc gttttccaga cgccgcgcgg ttgcagatcg ctgccgaggc tgacaccgga
     7921 aagcgttttg atcatggtga ccaggtcgct ggcgttacca taaaggcgtt tcccggtacg
     7981 ccacattttc tgcacctgct caacgaaatt tttgcctgac gatggtggcg gcagaagtac
     8041 cgagatatcc ccctgcaaca gcctggcggc atccgatacg gcagaatcca ccactttcat
     8101 cgcatcagaa acatacccaa gcattgtgct ggcattaccg acgacgtcgt tctgcacaaa
     8161 atctgccacg ccatcgatac tgaaaccact gaagctgtca ctgatgcagt catccagtgc
     8221 agaacaggat gacatcagcg tctgcgccgt cgccgcacct gaagtggggt aagagagttc
     8281 tcccgcttcg acaaacttca ggtcaaagcg gacaatacgc ccttcactct tcgatgtgct
     8341 gacccgaact tccccgtcaa cacagacttt cagctcaccg taagtcggat ggacaagcgt
     8401 gccgggaccg ggtttattca gcgcgtcaat caggcgatcg cgctggtcaa agcagtcatc
     8461 tcccaccaca taagctgtga tggacgggcg gaaagtgatt ttccccaggt cttcggtata
     8521 gggtttgtcg cggttcgggt attcgtgcgt ttccacacga cgaccggttc ccgcactttc
     8581 ttcttcaacc ttaaacggca caccgcgaaa tgacgcgtcc tgaagtctgt ctttccacgt
     8641 catataaact ccgtacataa aaaatcccac cggagtggga ctcattaaca gattaatttt
     8701 tcattacctg ccaaagcgcg tatagccaac atcatggctg acatcaaaac cgctggatcg
     8761 cgtttccata acccgcatac ccggaggcga attcacaaaa gagaccttga tctcaccatc
     8821 aacttttggc gcagaagctt tgttaatcat gaagggattc gggcctgtgg caccggaggc
     8881 gttgtttgac tgagccggat ctaccgcctg ataaggtgtg tatccccgtg ccggtattcc
     8941 cgtcccataa gcatcataag cacccgcgcc ccactgcgca gagttaatgg catcgaccgt
     9001 gtcaccggaa ctgtcggtaa accactcaat aattggcttc agtttgtccc acatatcctg
     9061 aaaccactta acaaccggtc cccagttatt gattaccatc cccagcggcg accaggcaaa
     9121 aaccttcttc agaagttccc agccagcctc aaaataagga ccaatggttt cccagagctt
     9181 cttgaaataa ggtccgacaa catcccagtt agtgataatt aatcccgcag ccagggctat
     9241 cgccgtcgca atcattccaa tcggcgtcat cgacatgatc ctgctgacaa tactgatggc
     9301 accgccaacg cccatcaatc ccagtttcag aatcgcaaga ccggcagcaa gcccgacgac
     9361 gccgcgaata acccggggat tttcatccgc aaacttcgtg aatttttccc ctaactcccc
     9421 cagccattgc gtgatatttt tggcgtcacc agaaaatgcg ccgccaatag ccgcaaggcc
     9481 gttagttgcg gtccccgtca ttgcctccca caggttggac agcgtaccaa gctgggcctg
     9541 aacacgttta ttcaggctgg cctgtttatt catcttctgc tggatctgat cgtagccatc
     9601 ctttccttta tcgattagtg cattgaccac ctgaagggtt tcggcatcat caccaaatat
     9661 tgccttaagt acacctgttc gcttaacgtc ggtcagtttt cgcagctttg ccagttgcct
     9721 gaacatgtta tcaagaccgc caaaacttcc tttgccgtca gtaaaatcga gctgtacccc
     9781 gagtttctgg cgggccatga ttttattgac gtccctgatt ttcttaacgc ttaatccgga
     9841 ctggataact tttcgcaggg cgttacctgc cgactccccg ttcatcccca tctgatccat
     9901 catgacgctg atgggggcaa ggctctgtgc agcctgaaga ccgtccttgt tcaccatctt
     9961 cagaacagag ctggttttag tgaagaagga caacatgttg gtatcgtcaa cacccagata
    10021 aaacgccttc tggatagtgt cgaacagccc catcatgtct tctgacgccg ttccggtagc
    10081 atcctgcatc tttgcagcaa actcagcagc cgcttccggt gtttttttca gttgtaccgc
    10141 aagataagct gtcgctttac ccacaccgcc aagaatgttt tctgccggga tcccctgacg
    10201 caccagcatc tgcatcatgt tctggaaatc agccgttgta ccgggtagct ggttacccag
    10261 gccaatagcc agtttattga tgtcctgaaa gctctttccg acctcgccgt tcgcatccat
    10321 catggcgact ttcagcccgg tggcggcgtt ttcctgatcg gcataagatt tcagggaaag
    10381 cgtcagaccc gctgccagtc cgccaccaag cgccagccca ccctgtgacg cttcttccgc
    10441 ctggcgttta aatccccgga ttttcttttg cattttcgac agcgcgggag aaagcctgtc
    10501 gacaccggtg atcaacgcct taagctcaaa ttccgccatg tgtgcgtttc tcctgctcta
    10561 tcctgtttgc ctgactgacc agcaaggtaa tttcactgat cggcatattc agcaattcga
    10621 aaggattaat gcgccagtaa ctggcgcagt caaagaagcg atcagtgagg tattcagccg
    10681 tcaggcctgg aggaaaaaac cagccacaag ccacgccgct gcattcaggt ctgccggaga
    10741 catctggtcg acagagcttt gcggcacttt cgccagccgc acaatgtatt tcgacaccac
    10801 atgcgccaga agtctgactg actcatcctg attcatctgg tagggatacc ccagctcgcg
    10861 gacatccttc ccggtgggtt catcaaactc cagtacggag agtgtctcgc catgagcagt
    10921 aatcggtttc tttaactcaa gctctttcat tactggtaat ccccttcttc accgtggaac
    10981 tcaagatcaa ccgtgccttc ttcggcatta tggttcgctt cgccgtgcag ccaggcagac
    11041 gacagtacat agacctgacc gttcgccagc tcggcagtga tggtcatctc atcagacgag
    11101 gtgattttgc ttaccggaaa attcttcggc accttgaagg tccctttgac ataaggcgca
    11161 cggtgagttt ccttgcggtc cactgaaccg tccaggccga tgatgtcatc gttaaccgtt
    11221 ttgttcatgg gcacctcaat gccgccggtc agcgatagct gctgaccgtc aattttgaaa
    11281 taacaggttc ccccgatacg ggccattatg cagactcctc tgaatactga agacggaact
    11341 gattaaccac ggcaaagaca cgcagctggt taacatagtc aggcgggaac agcgtgttca
    11401 ggcggttcgg atcgctggca tcacgctcca caaccaggta ctgcttaaac agttcgtagt
    11461 tttccacgat ccccgcacgc tcaagctgac ggtaggttgc cagcagttcc cctttgatca
    11521 ccgccggggt gacaatcgcc tgaccgggac caaagcgggt accgtcactg gcaagcttgt
    11581 gacgcccgta cttactggta atgacggatt tcagtttgcg cagtacatac gcgctggtat
    11641 gcagcgtctc actgtcgagg taactgttat ccgcaacccc gtaagcgttt ttcctgtacg
    11701 tggtgacatc acgctgaatg cgtagtaccc cgctttcgac atacgccgtt gccacgccat
    11761 gagacagcag ggtctgttgt tcggtcatcg tgaaccgttt ccccttcggc gcaggcagca
    11821 tacccaccag ctcaccggtc tgcgtgggac gtgccggatc gttgcggata aacaccgctg
    11881 cgcgggcggt acggcttgcc gccagctcgt cggcaggcgt ctgggtctct ttttcgtacc
    11941 ccgccagggt aatgtgctgc tggttaaact ggtcacctgc ggtcaccagt tctgacagcg
    12001 tgccgatctt tgccgtatac acatgaccat acagctgacg cgcatagctc cagcgaccgc
    12061 tggtatcgtt catctcggtc accagcgtgt taacggaggc cgtgtcgttg aacggcaggc
    12121 cgatataatc aaacggctca tccgccattg cagccaccgc gccggtgaga accggagcac
    12181 ccgttccggc ggtacccgtc gccacggcaa tctgtacgcc cgctggcagc acttcgcccc
    12241 caccaaagcc gtagtaattg aggctgacag gaatttcatt cccacaaagc cccttatgac
    12301 gcgcggtcag tgtgaccacg cctgccgaag atgaagccgt aaacggcagg gtcggaacgg
    12361 cattgatggc atcctagata ctgctggcaa tcatcgtgac gttatcgccg ttagtcaccg
    12421 gtgcctgcac gcgggtacgt cctacataca cattcaccgt gccggtttcg gttgccgccc
    12481 cggtcaccgt cagcgtaact gttgccgccg cacctgtgga ttcaggaacg gcaatcacat
    12541 acagctcgcc aaacgggtca gtctggcgat aagcctcgac catacgcgcc agctgacttc
    12601 ccgcaccaca aatctggcgt gcatagtctg ccgacgacat cagtaccaga ctgttggcaa
    12661 caatctctgc accgttattg gcatgaccaa tcagcagcga tgctccgctg tcctgtgcag
    12721 tattcgccgc ctggttatcc atttccgcat aaaacaacgg aaccagcgta ttcgacggaa
    12781 tggtgttaaa gcttatcgtc atcggtgttc acctttttat tcacgcgccg gatatcaccc
    12841 gctgcttcac ggcgcagcca gtagttgttc tcgtcaacat ttcgcccttc ggcgggcaaa
    12901 aggtcgccgc gggcagggtc aggcactgac cgccctttaa caggtttgac aaacatgagg
    12961 atcctcagga aggaagggtt atttaggtgt gatgttcgat atcgccgtca ggcccgttac
    13021 cgggctcgag ataatcaaca tcaatcgcca gcgtttgcag ttcatccaga ctgttcagat
    13081 catcctgctg gcgggtatcg tcttcagtca gctcgctgat gaccgaaaaa tcgaactgat
    13141 aaatcagctc atgacgattc agatccagca gcgtgccgcc gtcataggta atcgggttac
    13201 cgcacgcctc cgggttccag cccagcagag ccttaaagag catctgccgg acatcgtcca
    13261 ccacatcata cgaggcaaac tgaccacgct catcacgccc attactcagt atgacaacca
    13321 cggagaaacc ctctttcagc tcctgccagt agtcggtctg gcttttgttt tctcccggag
    13381 aatcatcacc cggtacaaca tatgccgccg ggagtttcag ctttccgacc tccggcagat
    13441 ttttgaactg ggccgcgcct gcaacccggt tttcaaaata cggacaacgg gcacgcagtg
    13501 cagcaataac aggagtcagt ttcatctgtg tcgtcgctcc ggcttcagtg atttacgcaa
    13561 ttcccgcgcc agaaaatagc gtgtccagct gcggttcttt tcaagcgttt ccaccatgaa
    13621 gttattacgt ggagccagtc gccagccgct gccaccggat gcaccacgat gatgactacg
    13681 acgacgtttt gctcctcccc ggacaccaaa aaacagaaac gccggataga agtcaccaga
    13741 gatcatccgg ttccccttcc cgttgcgctg gttaggggca atgcgtgtca taaaaccggc
    13801 tcgcttttta ctggctctcg gcaccatata accaatcgaa cgagccaggc gtccggtctg
    13861 ataaccgggg ttttcacccg gtgccgaccg cgcacggcgc atcaccagcc gacgggcatc
    13921 acgcatatga cgctgcccaa tcgtgacaaa cgcccgccgg acacgagcgc ggttaaagcg
    13981 catctcggcg ggctgctgaa catcaacgtg aaaaaaggga gtcgccattg ctgcctccgt
    14041 gactctgcct acattcgccc agctccgtac actccagcag cagaaagcgc cgcgccccgt
    14101 tcagatcacg ttgacgtttc acccggtaca cactgtcatc acagaccacc tcataatcag
    14161 cagtgatccc ccggcggtaa cgaatggtga tgtaatgggt gatggcgtcc ccggtctgcg
    14221 cggtttcctg ccaggtggtg gcactggtct ggataacctt cgcccatgtc cggaacgtaa
    14281 ccgggtattg aggctccacg ccaaagttat ccgcgggcat atccacccgc tggcggatca
    14341 ggacgcgttt attcagttca ccggggtccg gcagaatgta ggttgcgctg gtctgcgcct
    14401 gacgaatttt cattgcggaa agtacctgta cgggccgaca agccagccaa aactctgcgg
    14461 catgtcgagt ttctccactt ccgtaaccga cgagcggttt tcgtaaaaat ggctgataag
    14521 catcagcatc cccagacgaa tatcatccgg caggtgcagc ccgtccggat cgctgtccgg
    14581 aatggtttca tccggtgcat agagcttccg gttcagatac gtttccgtcc gcttttgtgc
    14641 cgcacaggcc agcagttgca gatggcggtc atcagcatcg aaatcctcat ccagccggag
    14701 ttgggcttta atctcttcca ttgtcagaag catactcagc cctctttact ggtcgtggct
    14761 tttttctctt ttgtcgcttt actgcttttt gcactggttc cgcgctctgc taacccggcc
    14821 tgaagtgcaa tctcctgcac ccgggcagga agcgccccgt cgtcatactc accggcccga
    14881 atgacctcaa cacgcatacc gtccggtgac catttcagat cttgtttcag gatcatgatt
    14941 cttcacccgt cagaacaggg gcgcggttcc gcgcccctga gtgattacgc cactgcaatc
    15001 ttcagcagtt tgatggcctg cgaatcgacc agcatgccgc cggtgcgttt ggtggtataa
    15061 aaaccgacaa acggtttatt ggtgtacggg tcacgcaga
//
LOCUS       DAJTSL010000093        15033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTSL010000093 DAJTSL010000000
VERSION     DAJTSL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.093"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(47..232)
                     /locus_tag="OO433_002607"
     CDS             complement(47..232)
                     /locus_tag="OO433_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6051031.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLY"
     gene            complement(229..2868)
                     /locus_tag="OO433_002608"
     CDS             complement(229..2868)
                     /locus_tag="OO433_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6051032.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFEYQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3076..4065
                     /gene="ldhA"
                     /locus_tag="OO433_002609"
     CDS             3076..4065
                     /gene="ldhA"
                     /locus_tag="OO433_002609"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6051033.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4176..4598
                     /gene="hslJ"
                     /locus_tag="OO433_002610"
     CDS             4176..4598
                     /gene="hslJ"
                     /locus_tag="OO433_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6051034.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4595..4861)
                     /locus_tag="OO433_002611"
     CDS             complement(4595..4861)
                     /locus_tag="OO433_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6051035.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5135..8659
                     /gene="nifJ"
                     /locus_tag="OO433_002612"
     CDS             5135..8659
                     /gene="nifJ"
                     /locus_tag="OO433_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6051036.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DSYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVEGNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9025..10158
                     /gene="ompN"
                     /locus_tag="OO433_002613"
     CDS             9025..10158
                     /gene="ompN"
                     /locus_tag="OO433_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR6051037.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGFSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10299..10733
                     /gene="uspF"
                     /locus_tag="OO433_002614"
     CDS             10299..10733
                     /gene="uspF"
                     /locus_tag="OO433_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6051038.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11290..12204
                     /gene="sitA"
                     /locus_tag="OO433_002615"
     CDS             11290..12204
                     /gene="sitA"
                     /locus_tag="OO433_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6051039.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12204..13031
                     /gene="sitB"
                     /locus_tag="OO433_002616"
     CDS             12204..13031
                     /gene="sitB"
                     /locus_tag="OO433_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6051040.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGYMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            13028..13885
                     /gene="sitC"
                     /locus_tag="OO433_002617"
     CDS             13028..13885
                     /gene="sitC"
                     /locus_tag="OO433_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6051041.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            13882..14739
                     /gene="sitD"
                     /locus_tag="OO433_002618"
     CDS             13882..14739
                     /gene="sitD"
                     /locus_tag="OO433_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6051042.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         3791 a         3770 c         3653 g         3819 t
ORIGIN      
        1 cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc atcgcctcag taaagatcgc
       61 tacgagtttc aagcagctct tcgacatctt tgtctgcctt gatgatgatc tcatgctcaa
      121 ttttaacgtt catattgata gtgatcggtt ccttgggcgc agcgacttca atacgcggcg
      181 tacaggcagc cagcataaaa gatgacgtca acgcagccag taaaattttc attgtttttc
      241 ctcacactct tttccttgcg gacagtcatt tcccggcaga cgtgcgttct gctccaacca
      301 tgcctggaga ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat tctcttcatg
      361 atggtaatta aggtttaccg taccgctttt accatcaacg cgactggtac ctgtaatgtt
      421 ggcctgcatg gttaataaac cgagattatc taaattaata tttgtcgatg aacggctaat
      481 ttccatatag cgcaaccagt taattgctga acccgcagtc atattgtctt tcaccaccgc
      541 atctgcggtg tctttatcca gccgcaaggt cattggcccg ctatttgcca gccaaccatc
      601 tttcactatc catttttcgt tgtttagcca taacggcaat gcaccactaa atgccccgga
      661 catggcgaat tgtttcggat tgacggcgct aaccagttcg ctggatgaga gattattcag
      721 ccgcaacagc gccgggtcat gttgcggcat acgtaattgt ttcatcagta cgttaccgcc
      781 taacacatcg acgctaacat cagtcaacag caagggttct tcttcagtcc acggataacg
      841 cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt cggcaattcg
      901 caacgtaacg gggccgcgag tccccagatg ccaggctcca tcggcaaaac ggaaaggcag
      961 gacaaaatcg acaccgttaa cctgattatc tggcatccag gcactaccgc ctttcaacac
     1021 gccgtgccct cccgcgcgga atccttgttc aggcgcagca gaaaatgcaa cctgagcata
     1081 cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc accagcggct ggaatacagt
     1141 cagtgattgt tttggccacc aggcgttacc gcgcagacga ataccgtccc agcgaccatt
     1201 tacccgaacc gggccaatct caccagcatg taaatcgcct ttaaagagga aatacgtcgg
     1261 atcgcgccca tcgacgctaa attttaaggt tgatggcggt aacacactgc cgccagtgaa
     1321 cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag gagggatgct gtgcgtcacg
     1381 tacccagacg acgggcttgt ccagaattaa tcgcggcttt tctaccgtca tcgtaccgta
     1441 ttgtaactgg tcgaaaccgg tggagaggtc ggtcagcgta atcgtgctgt catgccattc
     1501 accttttcct gcgacatccc aggtggcatt catcggtgta aaactgcctt ttccccagta
     1561 gcgccactgc cagcggcccg cgtcagggag aaaatcattc gccagcccat ccatatgcag
     1621 cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaac gtccgtcaac
     1681 accacgttgg gtgactttta cacccgctaa aggccagcgg atttcatcga tgtccagcga
     1741 atcgatgact cttccctttg aacgaagtaa cgcgcctggc tcaaaggcca gcgttgggtc
     1801 agtcagactt ccacttagct gcgcgggtaa gcgggcatat aaaatgaggt ccgcctgttt
     1861 cgcttcaccg gtcagctgca gaggcagctg gctgttatcc atgcttaatt ttcctgggcc
     1921 aaaatttagc accgcgttgc ctttacccgc ttgcccctgg gtcagcacac tcagtcgtcc
     1981 gctgatcaga gcgttctcaa gccctgcctg ccagttgtcg actttgacac ccagtcggcc
     2041 actcaaagga aaacctgcat acggccaact ccagcgacca tcgcttacgg tcaattgttg
     2101 acgagtaatt tgccacggca aatcgagcaa cggatcgccg ttatcacgtg ccagcacaat
     2161 caattgcccg ctattttcct gccagtccag ctctgcatcc accagtgacg gttcctgcgg
     2221 caagtttaac gtcgcagtag catgcccact tacaggaagt ccatccggca cgagcggcat
     2281 agtaaattcc cccaccagtt taaccggcgg ctgatattca aacgcgatga catccagttc
     2341 gctgactgta agttgttgcc ctttcagctg gccttgaaat ttaacttttt cgccctgata
     2401 acgcagttgc tggatatcag aggttaatgc gagagagagt tttccctgcc attcctgcca
     2461 gggagaaaaa atcagtttat cgatattgat ccaggtgtta ggcagcatgg actgccactg
     2521 cgcgagggtt tttggagcag ctggcgattg ctccgtctgc ggcaatttcg ccagacaagc
     2581 agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg aaaggctggc
     2641 gttggtgata tgcgcaagct gacaatctcc caccagataa cggagatcgg gaatgattaa
     2701 acctttacgc gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga gcggtagcca
     2761 gatgcccgcc agcgtgggaa cccacagccc gagcgtcatc agcagcgtca acggcacaag
     2821 aataatcagt aataacagcg cgagaacggc tttatattta cccagcatgg gtagttaata
     2881 tcctgattta gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt taaccgttca
     2941 gttgaaggtt gcgcctacac taagcatagt tgttgatgaa tttttcaata tcgccatggt
     3001 tttcaattat atttgaaatt ttgtaaaata tttttagtgg cttaaatgtg attcaacatc
     3061 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
     3121 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
     3181 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
     3241 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
     3301 tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag aactggggct gaaagtagtc
     3361 cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg
     3421 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
     3481 ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
     3541 ggtgtggcga tgctgcgtat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
     3601 tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
     3661 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggcaaacta ccatctgttg
     3721 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
     3781 gcattgattg attctcaggc agcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
     3841 ggtatggacg tgtatgagaa cgaacgcgat ctgttctttg aagataaatc caacgacgtg
     3901 atccaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctgtt taccgggcac
     3961 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
     4021 agcaatctgg aaaaaggcga aacctgcccg aacgaactgg tttaatgttg cagctcccct
     4081 gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct attcttaaaa
     4141 tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaagtagcc gcgtttgttg
     4201 cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt acgccagaac
     4261 agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg accagcgata
     4321 aaaatccgcc agaaatcagt tttggtgaaa aaatgatgat ttccggcagc atgtgtaacc
     4381 gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg ctggcaatga
     4441 cccgtatgat gtgcgctaac ccgcagctta atgaactcga taacaccatt agcgaaatgc
     4501 tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg accgcgaaac
     4561 agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag ctcccggcgg
     4621 caagtgactg ttcgctacag cgtttgccgt tgggtaatgc acacatccca atcgccgtgc
     4681 catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt gcttctccac
     4741 tactggacat cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc tgctgaacag
     4801 gttcactact acacgccgac aacaataaag cggcacaccc tacccaaaac gctgctcgca
     4861 tcttttcttc ctctgatctt caagccaaac gacaccgcca taaataatag gcagcacaga
     4921 gggcgacatc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt atggctattt
     4981 ttttgccctc tgtttgatca aaacattcat tacgctgatg tgggggacac aaaagcgaaa
     5041 atgcagaaga aagccatttg ctaaaattga aagattacta ctgggcgcgc agcaatttcg
     5101 tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg gtaatggcgc
     5161 ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc atctacccta ttacccccag
     5221 ttccacgatg gcagaacagg ctgatgcctg ggccggaaac ggcttgaaga acgtttgggg
     5281 agacacacca cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta ccgtgcatgg
     5341 cgctttgcag acgggtgccc tttcaacatc gtttacgtca tcgcagggtt tgctgctgat
     5401 gatcccgaca ctgtacaaac tggcaggcga actcacaccg tttgtcctgc atgtagcggc
     5461 acgtaccgtt gccacacatg cactctctat ttttggcgat cattccgacg tcatggcggt
     5521 gcgccagacg ggttgcgcga tgttgtgtgc ggcaaacgtc caggaagcgc aagactttgc
     5581 tctcatttcg cacatcgcga cgctgaaaag ccgcgtgcca tttattcatt tctttgatgg
     5641 tttccgcacg tcccacgaaa tcaataaaat tgtcccgctg gccgatgaca cgattcttga
     5701 tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg gcgctcaacc cggaacatcc
     5761 ggtgatccgt ggtacgtccg ccaatcctga ctcttatttc cagtctcgcg aagccaccaa
     5821 cccctggtac aacgcagtct atgaccatgt tgaacaggcg atgaatgatt tctctgccgc
     5881 gacaggtcgt cagtatcagc cgtttgaata ttacgggcat ccgcaagctg aacgggtgat
     5941 tatcctgatg ggctcagcca ttggcacctg tgaagaagtg gttgatgaat tgctaacccg
     6001 tggcgaaaaa gtcggcgtgc tgaaagttcg cctgtaccgc cccttctcag ccaaacactt
     6061 gctacaaact ctaccgggat ccgtacgcag cgtggcggta ctggacagaa ccaaagaacc
     6121 cggtgcccag gcagaaccgc tctatctgga tgtgatgacc gcactggcag aagcctttaa
     6181 taatggcaag cgcgaaactc tgccccgtgt cattggtggg cgctatggtc tttcatccaa
     6241 agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg ctaaaccgaa
     6301 agcgcgcttt acggttggta tttacgatga tgtaaccaat ctgtccctgc cgttgccgga
     6361 aaacacccag ccaaactcgg cgaaacttga agccttgttt tatggccttg gtagtgatgg
     6421 cagcgtttcc gcgaccaaaa acaatatcaa gattatcggt aattccacgc cgtggtacgc
     6481 acagggctat tttgtttacg actccaaaaa agcgggcggc ctgacggttt ctcaccttcg
     6541 cgtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt ttgttggctg
     6601 ccaccagttg cagtttatcg ataaatatca gatggctgag cgtttaaaac ctggcggcat
     6661 tttcctgctc aacacgccgt acagcgcaga tgaagtgtgg tcgcgcttgc cgcaagaagt
     6721 tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg attaacgcgg cgaaaatcgc
     6781 ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt ttttccatct
     6841 gacgcaaatt ctgcctggcg atagcgccct cgcagaattg cagggtgcga ttgccaaaag
     6901 ttacagtagc aaaggccagg atctggtgga aggcaactgg caggctctgg cgctggcgcg
     6961 tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg cacagcgcca atcgaccgcc
     7021 agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta accgcagcga tgctcgcagg
     7081 gcttggtgat gccctccccg tttcggcgct gccgccagac ggcacctggc cgatggggac
     7141 cactcgctgg gaaaaacgca atatcgccga agagatcccc atctggaaag aggaactctg
     7201 tacccaatgt aaccactgcg tcgccgcttg cccacactca gctattcgcg caaaagtggt
     7261 accacccgaa gcgatggaaa acgcccctgc cagcctgcat tcgctggatg tgaaatcgcg
     7321 tgatatgcgc gggcagaaat atgtcttgca ggtggctccg gaagattgca ccggttgtaa
     7381 cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta aagccatcaa
     7441 tatgatgtcg cgcctggaac atgtcgaaga agagaaaatc aattacgatt tcttcctcaa
     7501 cctgccagaa atcgaccgta gcaaactgga acgtattgat attcgtacat cgcagctgat
     7561 tacaccgctg tttgaatatt ccggtgcttg ctccggttgt ggcgagacgc cgtatattaa
     7621 attgctgact cagctctatg gcgaccggat gttgatcgct aacgccaccg gctgttcttc
     7681 aatttatggc ggtaacctgc cctctacacc gtataccacc gatgccaacg gtcgtgggcc
     7741 ggcatgggcg aactctctat ttgaagataa tgccgaattt ggccttggtt tccgcctgac
     7801 ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat caatttgccg ataaaatccc
     7861 ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca gaggttcgtc gagaacaggt
     7921 cgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaactgc tacgtgatgc
     7981 agatgcactg gtggaaaaat caatctggct gattggtggt gatggctggg cttacgatat
     8041 cggctttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca ttctggtgct
     8101 ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg aaagcgacac cgctgggtgc
     8161 agtaactaaa tttggcgagc acggcaaacg taaagcgcgt aaagatcttg gcgtcagtat
     8221 gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc tgaatcagac
     8281 ggtgaaagcg attcaggaag cggaagcgta tccgggacca tcgctgatca ttgcttatag
     8341 cccgtgtgaa gagcatggtt acgatctggc actcagccat gaccagatgc gccaactcac
     8401 agctaccggc ttctggccgc tatatcgctt cgatcctcgt cgtgccgatg aaggcaaact
     8461 gccgctggcg ctggattcac gcccgccgtc agaagcactg gaagaaacgt tacttcacga
     8521 gcaacgtttc cgtcgtctga attcgcagca gccagaagtg gcagaacagt tatggaaaga
     8581 tgctgcagct gatttgcaaa aacgctatga cttcctggca caaatggccg gaaaagcgga
     8641 aaaaagcaac accgattaaa tgctctggat aaggattatc caattctaaa aaaaagcccg
     8701 gacgactgtt cgggcttgtc tttttatatg ttggaaaatc agtggcaatg caatggccca
     8761 acagaaaata aaatctgtga caataaaggc atataacccg cgcagaataa cgtatagaaa
     8821 attaaaagta taatttttat tttgtatgta ttatcttcga tacattcacc agacttatta
     8881 tcatttcaga agaattattt gattaaggtt ttacttaagg cgtaacaaat gatatttatc
     8941 ggctaactga acttctcctt taggatgttt tcactccccg gaatggggat aaaaggcaaa
     9001 taaaataact aaaggattta ttcaatgaaa agcaaagtac tggcactttt aattcctgcc
     9061 ctgctcgccg caggtgctgc acatgcagcc gaagtttata ataaagacgg caacaaatta
     9121 gatctgtatg gcaaagttga tggcctgcat tatttttctg ataattcagc gaaagatggc
     9181 gatcagagct atgcgcgtct gggttttaaa ggcgaaaccc aaattaacga tcaactcact
     9241 ggctacggtc aatgggaata caatattcaa gcaaacaaca ctgaatcttc aaaaaaccag
     9301 tcatggaccc gtctggcatt tgccgggctg aaatttgcag attacggttc tttcgattac
     9361 ggacgtaatt atggcgtaat gtacgacatc gaaggctgga ccgatatgct gcctgaattt
     9421 ggcggtgact cttataccaa tgcagacaac tttatgactg gtcgagccaa tggcgtcgcg
     9481 acttatcgta atactgattt cttcggtctg gtaaatggtc tgaacttcgc ggtgcagtat
     9541 caaggtaaca acgaaggagc cagtaatggt caggaaggca ccaacaacgg acgtgatgtt
     9601 cgccatgaaa acggtgacgg ctggggtttt tccacaacat atgatttagg catgggcttt
     9661 agcgctggtg cggcatacac ctcttctgac cgcaccaatg accaggttaa ccatactgcg
     9721 gcgggtggtg ataaagcaga cgcgtggact gctgggctaa aatacgatgc taacaatatt
     9781 tacctagcaa ccatgtattc agaaacgcgt aatatgaccc cgtttggcga cagcgattat
     9841 gctgtggcaa acaaaaccca gaattttgaa gtcactgccc agtaccagtt tgattttggt
     9901 ctgcgtccgg cagtctcttt cctgatgtct aaaggccgtg acctgcacgc tgccggtggt
     9961 gcagacaacc cggcaggtgt tgatgataaa gatctggtta aatacgccga tgttggcgcg
    10021 acttactatt tcaataaaaa catgtccacc tacgttgact ataaaatcaa cctgttggat
    10081 gaagatgaca gcttctacgc tgccaatggc atctctaccg atgatattgt cgctttaggt
    10141 ctggtttatc agttctaaat cctcctgccc gctgttatgg cgggcttttt ctgcttattc
    10201 ttcctccttt gatctaaatt aaaaatgtga actccatcat tacacaaaaa gtgtcatctg
    10261 gcgttacact ttatgcggat actaaaacag gaggttttat gaacagaacg attcttgtcc
    10321 ctatcgatat ttccgattca gaattaactc aacgcgtgat tagccacgtt gaggcagagg
    10381 caaagattga cgatgcagag gttcatttcc tgacggtaat accttcactg ccctactatg
    10441 cctctctggg tttagcgtat tccgcagaat taccggcaat ggatgacctg aaagcggaag
    10501 ccaaatcgca actggaagag atcattaaaa aatttaaact gccaaccgac agagtgcatg
    10561 tccatgttga ggaaggctcg cccaaagacc gcattctgga attggcgaag aagatccccg
    10621 ctcatatgat catcattgct tcccatcgac cggatatcac cacttatctg ctcggttcca
    10681 acgccgcagc tgtagtgcgt cacgcagagt gctccgtgct ggttgtgcgc tgacactaac
    10741 gcccgcacat tgctgcgggc tttttgattc atttcgcaaa tgtgctgaca ttttcccctc
    10801 taatccgtac catacacgcc actgttttta tatcagattt cttatgctgg gcattccggc
    10861 atgacgcata ctcttctgat gccatataac gaattgagtc gcttttaaat gtcgcaaaat
    10921 caagaaatta gtaagaaaga acaatacaac ctgaacaagt aagggcaaaa atcacaacta
    10981 tctgactcat aagtatttta tttatttttc aatatgttaa acattatcag cgacaacaat
    11041 aagctacgat aacccactct ttttttgccc catttttgcc atcaacaatt ttcctcataa
    11101 agtcatttca aagcctaata gctgctctac ttttttcctc aatatcatgt cgccagcagg
    11161 aaacaaccta atccctgttt taggcctcaa agctgttaat gataattatt ttcattttgt
    11221 ataggttatg ctataaaaca tagccgttgc tatgtttgtt ctaattttgc tcactatagg
    11281 tactaaatta tgcactcaat aaaaaaagta accatgctct tggggtgcct cgccctcacc
    11341 tgctcgatcg catttcaggc aagtgcaact gaaaaattca aggtcattac aacatttacc
    11401 atcatcgcgg atatggccaa aaacgtggct ggagatgctg cagaagtctc atccataacc
    11461 aagcctggtg cagaaattca tgagtatcag cctacccctg gcgatattaa acgtgcacag
    11521 ggggcacaac tgattctcgc caatggtatg aatctggaat tgtggttcca acgcttttac
    11581 cagcatctca atggggttcc agaagtaatt gtctcttcgg gtgtgacgcc agtagggatc
    11641 accgaaggac cctatgaggg caaacctaat ccccatgcat ggatgtcgcc agataatgct
    11701 ctgatttacg tcgataatat tcgtgatgcg ttgataaaat acgatccggc aaatgcacaa
    11761 acctaccaac gtaatgccga tacttataaa gccaagatta cccaaaccct tgcccccctg
    11821 cgtaagcaga ttacggaact ccctgagaat cagcgatgga tggtcaccag tgaaggggct
    11881 ttttcttatc tcgcacgcga tttggggcta aaagagcttt atctgtggcc gattaatgcc
    11941 gatcaacaag gaacaccgca gcaggtacgt aaggttgttg atatagttaa gaaaaatcat
    12001 atcccggcag tctttagcga gagtacgatt tccgataaac cagcgcgtca ggttgcgcgt
    12061 gaaaccggcg cacactacgg cggtgtgctc tatgtcgatt ccctgagcac agaaaacggc
    12121 ccggtaccta cgtatatcga ccttcttaag gttactacca gtacgctggt acagggaatt
    12181 aaagccggga aaagggagaa ataatgatgc aatctgcagg cattgtcgtt aatgatgtta
    12241 ctgtcacctg gcgtaacggg cacacagcac tgcgcgatgc atccttcaca gtaccaagtg
    12301 gatctattgc cgctctggtt ggggtaaacg gttccggaaa atcgacgttg tttaaagcga
    12361 ttatgggatt tgtgcgtctg accagcggaa agatatctgt tctgggtatt cccacgcgac
    12421 aggcgctaca gaaaaacctg gttgcctacg tccctcagtc agaagaggtt gactggtcat
    12481 ttcctgtact ggtggaagat gtggtgatga tgggccgcta tgggtatatg ggtatgttgc
    12541 gtatagccaa aaagagggat cgtcagattg tcaccgatgc gctggaacgt gtcgatatgg
    12601 tggattttcg ccatcgtcaa atcggcgagc tttccggtgg acaaaagaaa cgcgtctttc
    12661 tggcgagagc gattgcacag cagggtgatg tgatcctgct ggatgaacct tttaccggcg
    12721 ttgatgtgaa aaccgaagcg aaaatcatca gcctgttgag ggagttacgc gcggaaggca
    12781 aaacgatgct tgtctcaacg cacaatctcg gctcagtcac gacattttgc gattatacgg
    12841 tcatggtcaa aggcaccgtc ctggcaagcg ggccgacaga caccactttt acagccgaga
    12901 acctggagct ggcttttagc ggcgtactgc gccatgtcac acttaacggc tcggaagaaa
    12961 gcattattac tgacgatgaa cgcccttttg tggcacatcg accgtcagcg gtgcagaggg
    13021 aagcaagatg aacgtgctac tggaaccctt cagctatgaa tatatgctca atgcgatgtg
    13081 ggtctcggca atggtcggcg gtctctgcgc tttcctgtca tgctatctga tgctaaaagg
    13141 ctggtcgctg attggcgatg cgctgtcgca ctctattgtc ccgggtgttg cgggggcata
    13201 tatgctgggg cttccgtttt ctctgggagc cttcttttcc ggcggactgg cagcgggcag
    13261 catgctgttt cttaaccaac gcacccgctt aaaagaagat gccatcatcg gcctgatctt
    13321 ctcgtccttt tttggcctgg ggctgtttat ggtgtcattg aacccgacat ccgtgaacat
    13381 tcagaccatc gtcctgggca atattctggc aatcgatccg gcggatatcc tgcaactcac
    13441 tatcattggc attctctcga tcatcgtgct ttttttcaaa tggaaagatc ttatggtgac
    13501 ctttttcgac gagaatcacg cccgtgctat tggattacat ccgggcagat taaaaatcct
    13561 cttcttcacc ctgctgtcgg tttcgaccgt tgcggcgctg caaacggttg gtgcctttct
    13621 ggtgatctgt ctggtggtga ctcccggtgc aacagcatgg ttgttaaccg atcgttttcc
    13681 ccgcctgctc ataattgctg taactattgg cagtgtaacc agtttcctcg gcgcgtgggt
    13741 cagttacttt ctggacggcg cgacaggtgg aatcatcgtg gtggcgcaga cactgctgtt
    13801 tcttttagca tttgtatttg cgcccaccca tggacttttg gcaaatcgtc ggcgtgcgca
    13861 taaagcgctg gaggatcgct catgatggca ttattactgg agccactgca gttcaccttt
    13921 atgagtcacg cactgctgat ttcactggta gtttccatac cctgtgcgct gctgtcggtc
    13981 tttctggtcc tgaaaggttg ggcactgatg ggtgacgcga tgagtcatgc ggtttttcct
    14041 ggcatcgtgc ttacctggat tctggggtta cctctggcta caggggcatt cgtcgccgga
    14101 gtattctgcg cggtggcgac gggatacctg aaagacaaca gccgaatcaa gcaagataca
    14161 gttatgggga ttgttttttc tggcatgttt gccgcaggtc tgatcttgta tattgcaatc
    14221 aaaccagacg tacatcttga ccatattctt ttcggcgaca tgctcgggat aaccatcggc
    14281 gatataatcc agacggtgat tattgccggg ctggttacac ttgttattag cgtaaaatgg
    14341 cgggattttt tgctgttcag ctttgattat caacaggcgc aggtaagcgg tttgcatacg
    14401 agatggctgc attatgggct gctgtgcatg gtttccctga ccattgtggc gacgctgaaa
    14461 gcagtaggta tcattctctc tatttctctg ctcattgcgc ctggcgccat tgcagtactc
    14521 ctgacacaac gctttcatat tgcgctccta ctggcgactg gcatatcggt aatagtgtca
    14581 atgactggtg tctggctgtc cttttttatc gacagcgcac cagctccgac gattgtcgtc
    14641 ttattcgcgg tcgtgttcat catgacgttt accgttacca gcatcaacgc acgcacaaag
    14701 ggaaacgccc atacacagga tctgttatca cccaattagc acaatatccg aggacagcat
    14761 cgtccgccag cgtccgccag cctacagaag ttcggaaagc catggaaggt cgttgggagg
    14821 aggtacgaat tctatcatgc aaaaaatacg taaaatcgat aacgcctaga aatcattcaa
    14881 tactcgcact atcgaaagtt taccagccaa ccgcggcacg ttcctgcata cgacgtgccg
    14941 cagcttctgt tattccacga taaaattcct aaccgaattc aaatccttca gactattcag
    15001 ctcttccttc agctcccgct gacggcgata aat
//
LOCUS       DAJTSL010000094        14936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTSL010000094 DAJTSL010000000
VERSION     DAJTSL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.094"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..450
                     /locus_tag="OO433_002619"
     CDS             <1..450
                     /locus_tag="OO433_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR6051043.1"
                     /translation="QEVNAALFKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIA
                     DPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMT
                     LGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(491..1423)
                     /locus_tag="OO433_002620"
     CDS             complement(491..1423)
                     /locus_tag="OO433_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6051044.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            1787..4573
                     /gene="polA"
                     /locus_tag="OO433_002621"
     CDS             1787..4573
                     /gene="polA"
                     /locus_tag="OO433_002621"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6051045.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNPLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(4955..5587)
                     /locus_tag="OO433_002622"
     CDS             complement(4955..5587)
                     /locus_tag="OO433_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6051046.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRHSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6169..6678
                     /gene="yihI"
                     /locus_tag="OO433_002623"
     CDS             6169..6678
                     /gene="yihI"
                     /locus_tag="OO433_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6051047.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            6867..8240
                     /gene="hemN"
                     /locus_tag="OO433_002624"
     CDS             6867..8240
                     /gene="hemN"
                     /locus_tag="OO433_002624"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6051048.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8430..8540)
                     /locus_tag="OO433_002625"
     CDS             complement(8430..8540)
                     /locus_tag="OO433_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6051049.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8651..10060)
                     /gene="glnG"
                     /locus_tag="OO433_002626"
     CDS             complement(8651..10060)
                     /gene="glnG"
                     /locus_tag="OO433_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6051050.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10072..11121)
                     /gene="glnL"
                     /locus_tag="OO433_002627"
     CDS             complement(10072..11121)
                     /gene="glnL"
                     /locus_tag="OO433_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6051051.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLEIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11295..12704)
                     /gene="glnA"
                     /locus_tag="OO433_002628"
     CDS             complement(11295..12704)
                     /gene="glnA"
                     /locus_tag="OO433_002628"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6051052.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13077..14900
                     /gene="typA"
                     /locus_tag="OO433_002629"
     CDS             13077..14900
                     /gene="typA"
                     /locus_tag="OO433_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6051053.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDY"
BASE COUNT         3864 a         3744 c         3852 g         3476 t
ORIGIN      
        1 caagaagtga atgctgcatt gttcaaagag tatttaccgt tattacaaca aagtgagccg
       61 accattaaac aaccggtaag atggaagaac gcactcggcg aactaaatgc caatctggat
      121 atcagtattg ccgacccagc caaatcttca tcatccacaa acaaagatat caaatcgctc
      181 aattttgatg tgaagttacc gcttaatgtc gtcacagaaa ccgcaaaaca gcttaattta
      241 tctgaaggaa tggatgcgga aaaagcgcaa aagcgggctg ataaacaaat cagcgggatg
      301 atgaccttag gtcagatgtt tcagttaatc acgattgaca acaataccgc ctcgctgcaa
      361 ctgcgttata caccgggtaa agttgttttt aacggacagg agatgagcga agaagaattt
      421 atgtctcgtg ccggacgttt tgttcattaa aatactcact cgcctctgct cattcagagg
      481 cgagcaaaat ttacttgtcc tgccgctgtg atgacatcaa cgacgtcaga agtcggtctt
      541 tttcctgcca cagtgagttc agccattgct gaaaatggcg cttaaagctt ttgtcattga
      601 tgtagtcgcc atgtaactca tcggcaatag gctgtaaatc cacatgaacg acaattcgcg
      661 tcagtttacc gcttaacata tcgaagaacg gctgacggtt attgtccgga taacacagcg
      721 taacattaag cagtttatcg aattgtttac ccagtacatt tagcgccatc gcaatgcctg
      781 ccgcctttgg tggcaacaag ttttgaaaag tggagtgggt ttgctgatgt ttttcttgcg
      841 tgaaacggga gccttcaacg aaattgacaa tggtggtggg atgcaggcga aacttttcac
      901 aagaacgccg agtggtttca acatctttac cacgtcgctc cggatgacgt aataaataag
      961 cgcgggaata acgcttcata aacggcatat ccagcgccca gcacgccagg ccaagaaatg
     1021 gcacccaggc cagctgttgc ttgaggaaat atttattcat cggaatgtgc ttacgaaaca
     1081 gcacgcacag tacgacaata tctgcccaac tacggtggtt acaaataagc agataccagt
     1141 tcttcttact taacccttcc agcccgtgaa cttcccattg caggtgtggg ttcagatgca
     1201 gtaataccgc cagaccttca caccagcaat acatcataaa atcacaaaaa cgcgaaacct
     1261 ttcgccaaat gactggcaca ggtagcaata gttttacaat cccggcaatg atgatcggga
     1321 cggaacaaaa aatagtgacc aaaatggtca atacgatact cagcaaaagg gttatcgcag
     1381 cgagtattct cgtcataatg aatttattca aaaggttagc catagacagt gcgctaaaga
     1441 agcgcaaggc gcagatttta gcagaaaacg accgaaataa cggatgatcc ttaaggagaa
     1501 aaataattca tatctatcca cattagaaaa aatcccatta tctcaattat tagggatggc
     1561 tttattttta actacatgaa aaacaaagac aaacatcata ctgtaaaaag catgataata
     1621 aattaaaagc gatgtaaata atttatgcac aaagttatcc acatgacgat ttgcgagcga
     1681 tccagaagat ctacaaaaga ttttcacgaa aagcggtgaa aaactcatgt ttttatcctg
     1741 tctgtggcat cctttaccca taatctgata aacaggcacg gacattatgg ttcagatccc
     1801 ccaaaatcca cttatccttg tagatggttc atcttatctt tatcgcgcat atcacgcgtt
     1861 tcccccgctg actaacagcg caggcgagcc gaccggtgcg atgtatggtg tcctcaacat
     1921 gctgcgcagt ctgatcatgc aatataaacc gacgcatgca gcggtggtct ttgacgccaa
     1981 gggaaaaacc tttcgtgatg aactgtttga acattacaaa tcacatcgcc cgccaatgcc
     2041 ggacgatctg cgagcacaaa tcgaacctct gcacgcgatg gttaaagcga tgggactgcc
     2101 gctgctggcg gtttctggcg tagaagcgga cgacgttatc ggtactctgg cgcgcgaagc
     2161 cgaaaaagcc gggcgtccgg tgctgatcag cactggcgat aaagatatgg cgcagctggt
     2221 gactccaaat attacgctta tcaataccat gacgaatacc atcctcggac cggaagaggt
     2281 ggtgaataag tacggcgtgc cgccagaact gatcatcgat ttcctggcgc tgatgggtga
     2341 ctcctccgat aacatccctg gcgtaccggg cgtcggtgaa aaaaccgcgc aggcattgct
     2401 gcaaggtctg ggcgggctgg atacgctgta tgccgagcca gaaaaaattg ctgggttgag
     2461 cttccgtggc gcgaaaacaa tggcagcgaa gctcgagcaa aacaaagaag ttgcttatct
     2521 ctcataccag ctggcgacga ttaaaaccga cgttgaactg gagctgacct gtgaacaact
     2581 ggaagtgcag caaccggcag cggaagagtt gttggggctg ttcaaaaagt atgagttcaa
     2641 acgctggact gctgatgtcg aagcgggcaa atggttacag gccaaagggg caaaaccagc
     2701 cgcgaagccg caggaaacca gtgttgcaga cgaagcgcca gaagtgacgg caacggtgat
     2761 ttcttatgac aactacgtca ccatccttga tgaagaaaca ctgaaagcgt ggattgcgaa
     2821 gctggaaaaa gcgccggtat ttgcatttga taccgaaacc gacagccttg ataacatctc
     2881 tgctaacctg gtcgggcttt cttttgctat cgagccaggc gtagcggcat atattccggt
     2941 tgctcatgat tatcttgatg cgcccgatca aatctctcgc gagcgtgcac tcgagttgct
     3001 aaaaccgctg ctggaagatg aaaaggcgct gaaggtcggg caaaacctga aatacgatcg
     3061 cggtattctg gcgaattacg gcattgagct gcgtgggatt gcgtttgata ccatgctgga
     3121 gtcctacatt ctcaatagcg ttgccgggcg tcacgatatg gacagcctcg cggaacgttg
     3181 gttgaagcac aaaaccatca cttttgaaga gattgcgggt aaaggcaaaa atccactgac
     3241 ctttaaccag attgccctcg aagaggccgg acgttacgcc gccgaagatg ctgatgtcac
     3301 cttgcagttg catctgaaaa tgtggccgga cctgcaaaaa cacaaagggc cgttgaacgt
     3361 cttcgagaat atcgaaatgc cgctggtgcc ggtgctttca cgcattgaac gtaacggtgt
     3421 gaagatcgat ccgaaagtgc tgcacaatca ttctgaagag ctcaccttgc gtctggctga
     3481 gctggaaaag aaagcgcatg aaattgcagg tgaagaattt aacctttctt ccaccaagca
     3541 gttgcaaacc attctgtttg aaaaacaggg tattaaaccg ctgaagaaaa cgccgggtgg
     3601 cgcgccgtca acgtcggaag aggtactgga agaactggcg ctggactatc cgttgccaaa
     3661 agtgattctg gagtatcgtg gtctggcgaa gctgaaatcg acctacaccg acaagctgcc
     3721 gctgatgatc aacccgaaaa ccgggcgtgt gcatacctct tatcaccagg cagtaactgc
     3781 aacgggacgt ttatcgtcaa ccgatcctaa cctgcaaaac attccggtgc gtaacgaaga
     3841 aggtcgtcgt atccgccagg cgtttattgc gccagaggat tatgtgattg tctcagcgga
     3901 ctactcgcag attgaactgc gcattatggc gcatctttcg cgtgacaaag gcttgctgac
     3961 cgcattcgcg gaaggaaaag atatccaccg ggcaacggcg gcagaagtgt ttggtttgcc
     4021 actggaaaca gtcaccagcg agcaacgccg tagcgcgaaa gcgatcaact ttggtctgat
     4081 ttatggcatg agtgctttcg gtctggcgcg gcaattgaac attccacgta aagaagcgca
     4141 gaagtacatg gacctttact tcgaacgcta ccctggcgtg ctggagtata tggaacgcac
     4201 ccgtgctcag gcgaaagagc agggctacgt tgaaacgctg gacggacgcc gtctgtatct
     4261 gccggatatc aaatccagca atggtgctcg tcgtgcagcg gctgaacgtg cagccattaa
     4321 cgcgccaatg cagggaaccg ccgccgacat tatcaaacgg gcgatgattg ccgttgatgc
     4381 gtggttacag gctgagcaac cgcgtgtacg tatgatcatg caggtacacg atgaactggt
     4441 atttgaagtt cataaagatg atgttgatgc cgtcgcgaag cagattcatc aactgatgga
     4501 aaactgtacc cgtctggatg tgccgttgct ggtggaagtg gggagtggcg aaaactggga
     4561 tcaggcgcac taagattcgc ctgaacatgc ccttttttcg taagtaagca acataagctg
     4621 tcacgttttg tgatggctat tagaaattcc tatgcaacaa ctgaaaaaaa attacaaaaa
     4681 gtgctttctg aactgaacaa aaaagagtaa agttagtcgc gtagggtaca gaggtaagat
     4741 gttctatctt tcagaccttt tacttcacgt aatcggattt ggctgaatat tttagccgcc
     4801 ccagtcagta atgactgggg cgttttttat tgggcgaaag aaaagatccg taatgcctga
     4861 tgcgctatgt ttatcaggcc aacggtagaa ttgtaatcta ttgaatttac gggccggata
     4921 cgccacatcc ggcacaagca ttaaggcaag aaaattattc gccgtcctgc gtttcttcta
     4981 caggctgcat ctcgctaaac caggtatcca gtttctgccg cagcttgtcc acgccttgtt
     5041 tcttcaacga agaaaacgtt tcaacctgca catcaccgtt aaacgccagt acagcttcac
     5101 gcaccatatt caattgcgct ttacgtgcgc cgcttgccag tttgtccgct ttggtcagca
     5161 gcaccagaac ggcgatattg ctgtctaccg cccactcaat catctgctga tccaaatctt
     5221 tcagcggatg gcgaatatcc attagcacca ccagaccttg caggctatga cgtttttcga
     5281 ggtattcgcc gagcgcacgc tgccatttgc gcttcatctc ttccgggact tccgcataac
     5341 cgtacccagg caagtcaacc agacgcttgc cgtcagccac ttcaaacagg ttgataagct
     5401 gggtgcgccc tggggttttt gaggtacgag ccaggctttt ctggttagtc agcgtgttca
     5461 gcgcgctgga tttacctgcg ttggaacggc ctgcaaaagc cacttcaatt ccggtatcgg
     5521 aaggtaggtg gcgaatatca ggcgcactca tcacaaaatg cgtctgttga taattcaaat
     5581 tagtcaaagc ggtcgtctcc gtcagtcaaa gctttgtggc gattatacct gaaccataat
     5641 aaaaggcgga tttttcggcg tgagcgttgt aagtaaaaac catacgcttt gtgagacatt
     5701 gccgatagtc tttatgcgaa atagcagaga aaattctgcg atgcatgtca aataagctat
     5761 ataaatcagt gaattgcttt tatataaaac tctgaaaatg tgcaaatact gatggcggtt
     5821 gattgtttgt ttaaagcgaa ggcgtaaagt agcacccata gagcgaggac gctaacagga
     5881 acaatgactc aggatgaggg tcaggagcgc caggaggcga agacagagga ttgtcaggaa
     5941 gacaaacgtc cggagacgta attaaacgga aatggaatca acacggattg ttccggctaa
     6001 aggaaaaaca gggtgtgttg gcggcctgca aggattgtaa gacccgttaa gggttatgag
     6061 tcaggaaaaa aggcgacaga gtaatctgtc gccttttttc tttgcttgct ttctgttaga
     6121 ttccgccgca aatcgatact gaataaaacg gctaaaagac gaaccattat gaaaccatca
     6181 tcttcaaact cacgcagcaa aggtcatgca aaagcgcgtc gaaaaacacg cgaggagctg
     6241 gatcaggaag ctcgtgatcg caagcgtcag aaaaaacgtc gtggtcatgc gccgggcagc
     6301 cgcgcagcgg gcggaaacac cacgtcaggc agcaaaggcc agaacgcacc aaaagatcca
     6361 cgtattggca gtaaaacccc tattccattg ggcgtgactg aaaaagtcac caaacagcac
     6421 aaaccgaaga gtgagaaacc tatgctttca ccgcaggcgg agttggaatt actggaaacg
     6481 gatgagcgtc tggatgcgct gctggaacgt ctggaagcag gcgaaaccct gagtgccgaa
     6541 gagcaatcct gggtggatgc taaactggat cgtattgatg agttgatgca gaaactcggc
     6601 ctctcttatg acgatgacga agaagaggaa gaagacgaga agcaagaaga catgatgcgt
     6661 ctgctgcggg gcaactaacg gattgccgcc gtgggccttc ccgttctgct gataaccctt
     6721 ccggttatat gttatctggt gtggttattc gttaaactac agcggttgtc gcggcgacaa
     6781 aagtggctgc gcaaccggct gatgacccga aacgggcatc cgccggtacg ccgtagccgc
     6841 cagagacgcc atcggaagga gtgagcatgt ctgtacagca aatcgactgg gatctggccc
     6901 tgatccagaa atataactat tccgggccac gatacacctc gtacccgacc gcgctggagt
     6961 tttcagaaga cttcggcgaa caggcgtttt tacaagccgt ggcgcgctat cctgagcgtc
     7021 cattatctct ctacgtacat atcccgttct gccataagct ttgttacttc tgcggttgca
     7081 ataagattgt tactcgccag cagcacaagg ccgatcagta tctggacgcg ctggagcaag
     7141 aaatcgtcca tcgtgcaccg ctgttcgccg ggcgtcacgt cagccaattg cactggggcg
     7201 gcggaacgcc gacgtatctg aataaagcgc aaatcagccg cctgatgaag ctgctgcgcg
     7261 aaaacttcca gttcaatgcc gatgcggaga tttcgatcga agtcgatccg cgggaaatcg
     7321 aactggatgt actcgatcat ttacgcgccg aaggctttaa tcgcctgagc atgggcgtgc
     7381 aggacttcaa caaagaagtg caacgtctgg ttaaccgcga gcaggatgaa gagttcatct
     7441 ttgcactgct taaccatgcg cgtgagattg gttttacctc caccaacatc gacctgattt
     7501 acggcctgcc gaaacagacg ccggagagtt tcgcctttac cctgaaacgt gtggcggagc
     7561 tgaaccccga tcgtctgagt gtctttaact acgcgcatct gccgaccatt tttgctgctc
     7621 agcgcaaaat caaagatgct gacctgccga gtccgcagca aaaactcgat atcctgcagg
     7681 aaaccatcgc cttcctgacg caatcgggct atcagtttat cggtatggat cactttgccc
     7741 gtccggatga cgagctggcg gtggcccagc gtgaaggcgt gctgcatcgt aacttccagg
     7801 gctacaccac tcagggcgat accgatctgc tggggatggg cgtttccgcc atcagcatga
     7861 ttggcgactg ctacgcgcag aaccagaaag agttgaagca gtactatcag caagtggatg
     7921 aacaaggcaa tgcgctgtgg cgtggtattg cgctaacgcg tgatgactgt attcgccgcg
     7981 atgtgattaa gtcgctcatc tgcaacttcc gtctggatta cgcccctatt gagaaacagt
     8041 gggatttgca cttcgctgat tactttgcgg aagatctcaa gctgctcgcc ccgttagcaa
     8101 aagatgggct ggtggatgtg gatgagaagg gaatacaggt gacggcgaaa ggtcgcttgc
     8161 tgatccgcaa catttgcatg tgctttgata cctatctgcg ccagaaagcg cggatgcagc
     8221 agttctcacg ggtgatttga atagtgaatg gcgcttcgtt tacaaagtag aaaacgaaag
     8281 cgccatcaat gtgctgagaa caagattgcc tgatgcgcta cgcttatcag gcctacaaaa
     8341 cctgttgaaa atattgcttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
     8401 caaagagcac ttcatcagca atctgaaact caactaaaca gcccaatcat tgcggcacac
     8461 agcacggcag caacggcagt ttgcggtgtg tggctgtcaa ctgcgccact cgataccaga
     8521 ttaattattg attccagcat aatgactctc cccgttttcc gggcagatca tactgaactt
     8581 atcggaacag taaagcgtaa aataccagca atttgcgctc aataatcaat ctttacacac
     8641 aagctgtgaa tcactccatc cccagctctt ttaacttacg cgtcagggtg ttgcggcccc
     8701 agccgagtag ccgcgccgct tcctgtttat gcccctgcgt atgtcgcaac gcggtcgtca
     8761 gtaacgtccg ctccagctct ggctgcgctt cggaaagcag attttgatga ccggaacgca
     8821 gcgctctgtc tgcccactgc gcaagaagcg tcgcccagct gtccggttgc atttgcgaag
     8881 tactctccgc aaccgttgat tcaaacagtt cgccgggcaa atcctgaatc aacacttcct
     8941 gcccggcggc catcaccgtt agccagcggc aggtgttttc cagctggcgc acgttgcctg
     9001 gccacgccag acgcgtcaga gcagcttcgg tttccggatg cagtaacttc gcttctacgc
     9061 ccagttcgcg cgcggcaacc tgtaaaaaat ggcgcgccag acggggaata tcttcccgac
     9121 gttcgcgcag cggcggcaga tgaacgcgga taacgtttag gcggtggaac agatcctcac
     9181 ggaacttacc ttcctgcact cgctgttcga gattctggtg agtggcagcg ataatccgca
     9241 catccacttt caccggcgca tagccgccaa cgcggtaaaa ctgaccgtct gccagcacgc
     9301 gcagcaaacg cgtctgcaca tccagcggca tatcaccaat ttcgtcgagg aataatgtac
     9361 cgccatcggc ctgttcaaaa cgcccctgac gaatggtatt cgcgccagta aacgcgcctt
     9421 tctcgtggcc aaacagttct gattcgatca aatcttttgg gatagctgcc atattcagcg
     9481 cgataaacgg tgctttggcg cgcggactgt ggcgatgcag ggcatgagcg accagttctt
     9541 taccggtgcc ggattcgccg ttaatcagca cgctaataga agaacgcgaa agccgaccga
     9601 taatacggaa cacgtcctgc atggctggcg cttcgccgat gatatcggtc gttgggccgt
     9661 taagctgaat attacgcggc tgctgctgtt cctggtaatg actgatagcg cgctcaacca
     9721 gcgccacggc ttcgtcgata tcaaacggtt tgggcagata atcaaacgcc ccttgttgat
     9781 aggcgctgac ggcagcatcc agatcggaat gtgcggtcat aatgatgacc ggaagcattg
     9841 gatggcgctg tttaatctgc ttgagcagcg ccagcccgtc cattcccggc atacggatat
     9901 ctgaaagcag cacatccggc gttttgctcg ccagcgcctc cagtacttcc gcgccgttct
     9961 caaatgtcgt acaggttaaa cccgctccag cgagcgcacg ttcaagcacc caacggatgg
    10021 aactatcgtc atcgactacc cagactatcc ctcgttgcat aaacgtcacc tttatttcct
    10081 gataggcagg taaaccgaga actcggtatg acctggccaa ctggtaaatt caattttgcc
    10141 tgaatgctga tcaatcaaat tacgggcgat ggataagcca agcccggtgc caccttcgcg
    10201 gccgctgacc atcgggtaaa acagcgtatc ctgcaaatga ggcggaatgc ccggcccgtt
    10261 atcttccaca tcaatccgcg ccgccagccg gtagcgctcg ccgtgtaagg tcagttgaaa
    10321 cgcggtgcgg gtacgcagaa tgatttcacc gccttccggc cccagcgcct gtagcgcatt
    10381 gcgcacaata ttcagcaaga cctgttcaat ttgatccggg tcgtgcgcca gttccggcag
    10441 gctgggatcg taatcacgaa tcaaccgcac gttgtccggc agttccatcg acaccagcgt
    10501 taccacgcgt tcagccactt tgtgaatact ttcggtaacg cgcgtaccgg gcagctgcgg
    10561 ccccaacaga cggtcgacca gatttcgcag ccggtccgcc tgttcgataa tcactttggt
    10621 atattcgagt agtgatgggt caggtaacgc tttgctgagc agctgcgccg cgccacgtaa
    10681 accgccaagc ggatttttaa tctcatgtgc caggccgcgc actaaatcac gggcagcaac
    10741 ctgctgggcg tgctgtagct gttcctgact taagcggcgc tggttatcca tcggagccat
    10801 ctccagcagg atcatgccgt ccggcatacg ctgggccgtc acagaaagga tatgcgagcg
    10861 cccgtcgatg accagcgtca cttcgttatc ggtaaaacct tgccccgcct ccagactttc
    10921 ttgcatcagc tcgatattta atgagaagta gctcaacagt tccggtaacg gtgtaccaaa
    10981 caatttgcgg gagctttggg cgagcagttg ttgcgcggca gggttggcgt aatggatctc
    11041 caggttgtca tcgattaaca aaatactgtt aatcagcgag ttgaggatct gcccagcatc
    11101 gggctgcgtg cctgttgcca taaagcagtc tcctgaacag gttgcaccat tttagtgcat
    11161 tatagctttt tacggataaa aagcgcgaag catcagagaa ttgacggaga aaaaagccca
    11221 tgcagagatg ggctgaaagt ttccacggca actaaatccc ggcgttgttg cgccgggtag
    11281 tacaacttca actattagac gctgtagtac agctcaaact ctaccggatg cggagtcata
    11341 cgcacgcggt catcttcttc gcgacgcaga gcgatgtacg catcaattgc ttcgtcagtg
    11401 aacacgccac cggctttcag gaactcgcgg tccagatcca gttcgttcag tgcttcttcc
    11461 agagagcctg caacctgtgg gatctctttc gcttcttctg gcggcaggtc atacaggttt
    11521 ttgtccatgg cttcacccgg atggatcttg ttcttgatac catcaagacc ggccatcagc
    11581 agggcagcaa agcacaggta cgggttagct gccggatccg ggaaacgtac ttcgatacga
    11641 cgtgctttcg gagaagaaac caccggaata cggatagacg cagaacggtt acgcgcagag
    11701 taagccagca ttaccggtgc ttcatagccc gggaccagac gcttataaga gttggtggtc
    11761 gggtttgcca gggcgttaat cgctttagcg tgtttgatta cgccgccaat gtagtacagc
    11821 gcctgctcag acagacctgc gtatttgtcg cctgcgaaca ggttaacgcc gtttttagac
    11881 agagacatgt ggcagtgcat accggagccg ttatcaccga acatcggttt tggcataaag
    11941 gtcgcggttt taccgaagcg gtgcgctacg ttgtgcacaa catatttgta gatctgaatt
    12001 tcgtcagctt ttttggtcat ggtattgaag cgggtagcca cttcgttctg accagcagtc
    12061 gctacttcgt ggtgatgggc ttcaaccacc agccccatct gttccatcac cagacacatt
    12121 tcagaacgaa tatcctgagc cgagtctacc ggtggaaccg ggaagtaacc gcctttcact
    12181 gccggacggt gacctttgtt accaccttcg tattgggtgg aggagttcca tgcgccttcg
    12241 atatcgtcga tagcaacgtg ggaaccggag atagatgatc cgaaacggat gtcatcgaac
    12301 aggaagaatt caggttctgg cccgaacagt acggtgtcgg caatgccagt ggaacgcagg
    12361 taatcttcgg cgcgcttcgc aatggagcgc gggtcacggt catagccttg cagggtgcca
    12421 ggttcaagga tgtcgcaacg gataatcagg gtggagtcgg cgaagaacgg gtcaatcact
    12481 gcggtggatg cgtctggcat cagcaccatg tcggactcgt taatgccttt ccagccgcca
    12541 atcgaggagc cgtcaaacat tttgccttct tcgaagaatt cagcattcac ctgatgagca
    12601 gggatagtga cgtgctgttc tttaccttta gtatcggtga agcgcaaatc aacaaacttc
    12661 acttcgtgct cgttcagcat cgtcagtacg tgttcagcgg acatacttta actctcctgg
    12721 attggtcatg gtcgtcgtgg taacgaaatc tgcaattatt ttggccgtgt cgccgtaaaa
    12781 aagataaagc gaaatctgtg ccaactttta aattgcccct aaaaggcgtt atcatgcgca
    12841 ccatcgtgca aaagggctgc accacgatgt gaatgttgca ccaatatagt gcttcaatgg
    12901 aaacattaag caccatgttg gtgcaatgac ctttggataa ccctttttat gctccgtgaa
    12961 agcgatcaca aagggactct gcaatacttg tttgcggagg atgtttgtga tcctgttttg
    13021 tagtgcgatt aatccgtgta caataacgcg ctatttctaa tgcctgaggc aaagttgtga
    13081 tcgaaaaatt gcgtaatatc gccatcatcg cgcacgtaga ccatggtaaa accaccctgg
    13141 tagacaagct gctccaacaa tccggtacgt tcgactctcg tgccgaaacc caagagcgcg
    13201 tgatggactc caacgatttg gagaaagagc gtgggattac catcctcgcg aaaaacaccg
    13261 ctatcaaatg gaatgattac cgtatcaaca tcgttgatac cccggggcac gccgacttcg
    13321 gtggtgaagt tgaacgtgta atgtccatgg tagactcagt gctgctggtg gttgacgcat
    13381 ttgacggccc gatgccgcaa acgcgcttcg taaccaaaaa agcgtttgct tacggcctga
    13441 agccgattgt tgttatcaac aaagttgacc gccctggcgc gcgtcctgat tgggttgtgg
    13501 atcaggtatt cgatctgttc gttaacctcg acgcgaccga cgagcagctg gacttcccga
    13561 tcgtttacgc ttctgcgctg aacggtatcg cgggtctgga ccacgaagat atggcggaag
    13621 acatgacccc gctgtaccag gcgattgttg accacgttcc tgcgccggac gttgaccttg
    13681 acggtccgtt ccagatgcag atttctcagc tcgattacaa cagctatgtt ggcgttatcg
    13741 gcattggccg catcaagcgc ggtaaagtga agccgaacca gcaggtcact atcatcgata
    13801 gcgaaggcaa aacccgcaac gcgaaagtcg gtaaagtgct gggccacctc ggtctggaac
    13861 gtatcgaaac cgacctggcg gaagctggcg atatcgttgc gatcacgggc cttggcgaac
    13921 tgaacatttc tgacaccgtt tgcgacacgc aaaacgttga agcgctgccg gcactctccg
    13981 ttgatgagcc gactgtttct atgttcttct gcgttaacac ctcgccgttc tgcggtaaag
    14041 aaggtaagtt cgtaacgtct cgtcagatcc tggatcgtct gaacaaagaa ctggtacaca
    14101 acgttgcgct gcgcgtagaa gaaaccgaag acgctgatgc gttccgcgtt tctggtcgtg
    14161 gcgaactaca cctgtctgtt ctgatcgaaa acatgcgtcg tgaaggtttc gaactggcgg
    14221 tatcccgtcc gaaagttatc ttccgtgaaa tcgacggtcg taaacaagag ccatatgaaa
    14281 acgtgactct ggacgttgaa gaacagcatc agggttctgt aatgcaggcg ctgggcgaac
    14341 gtaaaggcga cctgaaaaac atgaatccag acggtaaagg ccgcgtacgt ctcgactacg
    14401 tgatcccaag ccgtggtctg attggcttcc gttctgagtt catgaccatg acttccggta
    14461 ctggtctgct gtactccacc ttcagccact acgacgacgt acgtccgggt gaagtgggtc
    14521 agcgtcagaa cggcgtactg atctctaacg gtcagggtaa agcggtcgcg ttcgcgctgt
    14581 tcggtctgca ggatcgcggt aagctgttcc tcggtcacgg tgcagaagtt tacgaaggtc
    14641 agattatcgg tattcatagc cgctctaacg acctgactgt aaactgcctg accggtaaga
    14701 aactgaccaa catgcgtgct tccggtactg acgaagccgt tgttctggtt ccgcctatcc
    14761 gcatgactct ggaacaagct ctggagttca tcgatgatga cgaactggta gaagtgactc
    14821 cgacctctat ccgtattcgt aaacgtcacc tgacggaaaa cgatcgtcgt cgcgccaacc
    14881 gcgcaccgaa agactattaa tttcgttttt tagttataaa aaacctgcca tcgctg
//
LOCUS       DAJTSL010000095        14557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTSL010000095 DAJTSL010000000
VERSION     DAJTSL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.095"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..423
                     /locus_tag="OO433_002630"
     CDS             <1..423
                     /locus_tag="OO433_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6051054.1"
                     /translation="TLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKR
                     LAEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMT
                     LSIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            439..921
                     /locus_tag="OO433_002631"
     CDS             439..921
                     /locus_tag="OO433_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6051055.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(998..1345)
                     /gene="rplS"
                     /locus_tag="OO433_002632"
     CDS             complement(998..1345)
                     /gene="rplS"
                     /locus_tag="OO433_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6051056.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(1387..2154)
                     /gene="trmD"
                     /locus_tag="OO433_002633"
     CDS             complement(1387..2154)
                     /gene="trmD"
                     /locus_tag="OO433_002633"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6051057.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(2185..2733)
                     /gene="rimM"
                     /locus_tag="OO433_002634"
     CDS             complement(2185..2733)
                     /gene="rimM"
                     /locus_tag="OO433_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6051058.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEDYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(2752..3000)
                     /gene="rpsP"
                     /locus_tag="OO433_002635"
     CDS             complement(2752..3000)
                     /gene="rpsP"
                     /locus_tag="OO433_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6051059.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(3137..4498)
                     /gene="ffh"
                     /locus_tag="OO433_002636"
     CDS             complement(3137..4498)
                     /gene="ffh"
                     /locus_tag="OO433_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6051060.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            4665..5456
                     /locus_tag="OO433_002637"
     CDS             4665..5456
                     /locus_tag="OO433_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6051061.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            5477..6763
                     /locus_tag="OO433_002638"
     CDS             5477..6763
                     /locus_tag="OO433_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6051062.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(6818..7411)
                     /gene="grpE"
                     /locus_tag="OO433_002639"
     CDS             complement(6818..7411)
                     /gene="grpE"
                     /locus_tag="OO433_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6051063.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            7534..8412
                     /gene="nadK"
                     /locus_tag="OO433_002640"
     CDS             7534..8412
                     /gene="nadK"
                     /locus_tag="OO433_002640"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6051064.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCKKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8498..10159
                     /gene="recN"
                     /locus_tag="OO433_002641"
     CDS             8498..10159
                     /gene="recN"
                     /locus_tag="OO433_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6051065.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMAYGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALATQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            10308..10649
                     /gene="bamE"
                     /locus_tag="OO433_002642"
     CDS             10308..10649
                     /gene="bamE"
                     /locus_tag="OO433_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6051066.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(10711..11001)
                     /locus_tag="OO433_002643"
     CDS             complement(10711..11001)
                     /locus_tag="OO433_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6051067.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10991..11428)
                     /gene="ratA"
                     /locus_tag="OO433_002644"
     CDS             complement(10991..11428)
                     /gene="ratA"
                     /locus_tag="OO433_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6051068.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            11599..12081
                     /gene="smpB"
                     /locus_tag="OO433_002645"
     CDS             11599..12081
                     /gene="smpB"
                     /locus_tag="OO433_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6051069.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            12296..12658
                     /gene="ssrA"
                     /locus_tag="OO433_002646"
     tmRNA           12296..12658
                     /gene="ssrA"
                     /locus_tag="OO433_002646"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12819..14557)
                     /locus_tag="OO433_002647"
                     /pseudo
     CDS             complement(12819..14557)
                     /locus_tag="OO433_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         3591 a         3907 c         3431 g         3628 t
ORIGIN      
        1 acgttgatga acaattccga tgcccgaaaa acgtcggcgt tactatttct tgatggcgat
       61 aatttcaaat atatcaatga tacctggggt catgcgacgg gcgatagggt cttgattgaa
      121 atcgcaaaac ggttagctga atttggcggg ctgcgacata aagcataccg cctgggcggc
      181 gatgaattcg ctatggtgct ctatgatgta cagtcggaat ctgaagtgca acagatatgc
      241 tcagcactga cacaaatctt taatctcccg tttgatcttc ataatggcca tcagaccacc
      301 atgacattaa gcattggtta cgcgatgacc attgagcacg cctctgcgga aaaattacaa
      361 gagcttgccg atcacaatat gtatcaggcc aaacaccagc gtgccgaaaa gctggtgaga
      421 taacaaggat atatcgatat gataaagcac ctggtagccc ccctgatttt cacctcacta
      481 atactgactg gctgccagtc ccctcaggga aagtttactc ctgagcaagt cgccgctatg
      541 caatcttatg gatttactga atccgccggc gactggtcgc tgggcttatc agatgccatt
      601 ctgttcgcaa aaaatgacta caaattgctc ccggaaagcc agcaacagat ccaaaccatg
      661 gcagccaaat tggcctcgac agggctaaca catgcccgta tggatggaca caccgataac
      721 tatggtgaag acagttacaa cgaaggctta tcattgaaac gggcgaatgt cgtggccgat
      781 gcatgggcta ttggtggaca aattccacgc agcaatctca ccacacaggg tttaggaaaa
      841 aaatatccca tagccagtaa caagaccgcc cagggccgcg ccgagaaccg ccgcgtcgca
      901 gtggtgatta caacccctta aaacacgagc aaacagacaa aaaaagggcc agccaattgg
      961 ccagcccttc ttaacaggat gtcgcttaag cgaaatctta gttaagacgc tctttgatac
     1021 gagcagcctt accagtacgc tcacgcaggt agtacagttt agctttacga acagcaccac
     1081 gacgtttgac agaaatgctg tcaactaccg gagagtgagt ctggaagaca cgctcaacgc
     1141 cttcgccgtt ggaaatttta cgaacagtga atgcagagtg cagaccgcgg ttacgaatag
     1201 cgataaccac gccctcgaat gcctgcagac gttttttgga accttcaaca acccatactt
     1261 tcacttccac ggtatcaccc ggacggaagg aaggtacgtc ctgcttcatc tgctcttgtt
     1321 caagttgctt aataatgttg ctcataattt aatctcttat cctgggtaaa ctgatatctc
     1381 gggggcttac gccatcccat catgtttatg ttgctgttgt gcgtgttccg ttttgaactc
     1441 cgccagcaac cttgcttgct cttcagtcag agccaggttt tccagaagtt caggtcttct
     1501 aagccaggta cggcccagcg actgtttcaa acgccagcga cgtatctcgg catggttgcc
     1561 cgacagtaac actggcggaa cttccatccc ttctaacacc tcaggccgcg tatagtgcgg
     1621 gcaatccagc aatccttcag caaaggaatc ttccgttgcc gaggcttcat gtcccagtac
     1681 ccccggaata aaccgggaaa cggagtcaat cagcgtcatt gctggtaact caccaccact
     1741 gagaacgtaa tcgccgattg accattcttc gtcaatttcg gtttggatca cgcgctcatc
     1801 tataccttcg tagcgaccgc acaccagaat caatttttga ttcgttgcca attcgctgac
     1861 gcccgcttga tcaagcttgc gtccctgtgg tgacagataa atcacctttg cgccttcacc
     1921 cgccgcggct tttgctgcat gaatggcgtc ccgcaagggt tgcaccatca ttaacatccc
     1981 cggtccgccg ccgtaaggac gatcgtccac ggtacggtgc cggtcatgcg tgaagtcgcg
     2041 aggactccag ctctggatgc tcagcaggcc atttttaact gcccggccag ttaccccgta
     2101 atcggtaatt gcgcggaaca tttcaggaaa caggctaatt atgccaatcc acatagcgcc
     2161 gtcttttacc gtttatccgg tggtttaaaa accaggatcc caatctactt cgattgaacg
     2221 agtagtgaga tcgactttct tgataacctg cccatcgagg aacggtacga gacgttcctt
     2281 gataccaaac gcatctttca ggtttgcctt aatgacgaga acgtcattag atccggtttc
     2341 catcatatcg acgactttac cgagatcgta gtcttcagtg gttactacct ggcagcccat
     2401 caggtctttc cagtagtagt caccctcttc aagctgaggc aactgcgatg aatccacgac
     2461 aatttcacaa ttcgtcagca ggttcgccgc atcacgatcg tcaacgcctt tcagcttgat
     2521 gatcatgtcc tgattgtggt gcttccagct ttccagctgg acttgctgcc actgacccgc
     2581 cttctggata aaccagggct gatagtcaaa aatgctttcg gcgtcttcgg tggaagaaaa
     2641 cactctgagc cacccacgaa taccgtaaga cgaacccatt tttcccaaaa cgatgggatc
     2701 aacaggtgct tgcgcggtga gttgtttgct catcatgacc accgtgacag attaagctgc
     2761 tttgtttact tctttgatca gcgcagcaac gcgatcagaa atagttgcgc cctggccaac
     2821 ccagtgagcg atgcgatcca gatccaggcg agtgccttct tctttttcgc tagcgattgg
     2881 gttgaagaaa ccaacgcgct cgatgaagcg accgttgcgt gcattacggc tgtcagcgac
     2941 aacaacctgg tagaacggac gctttttagc gccgtgacgt gctaaacgaa tagttaccat
     3001 aacatcctct tgtgtgaata aaacaaccgg accccatcga ggaacggagt ccggtgtcat
     3061 attaaaagcc cgaaaatttt actgatttct ggagaaaatg caatcagcag ttaataactc
     3121 tgtcgcataa ggtcatttag cgaccaggaa atcccggcgg catcatcccc ttcatgcttc
     3181 tcatcatctt cgccattccg cccttcttca ttttcttcat catgcgctgc atgtcgtcga
     3241 actgtttcag aagacggtta acgtcctgca cctgcatacc gcaaccggca gcaatacggc
     3301 gtttacgcga acctttgatg atttctggct tagcgcgctc tttcatcgtc atcgagttga
     3361 tgatggcttc catacgcacc agcactttat cgtccatctg tgacttgacg ttatccggga
     3421 tctgccccat gcccggcagc ttgcccatca ggctggccat gccgcccata tttttcatct
     3481 ggcgcagctg ctcaagaaag tcgttgagat cgaagccgtc accttttttc agcttgctgg
     3541 ctaatttctc tgcctgcgcg cggtcaactt tgctttcgat atcttcgatc agcgacagta
     3601 cgtcgcccat gccgagaata cgcgacgcga tgcggtccgg atggaacggc tccagcgcct
     3661 cagttttctc gccaacaccg aggaacttga tcggtttgcc agtgatgtga cgaatagaga
     3721 gcgccgcacc gccgcgggca tcgccgtcca ctttggtcaa cactacgccg gtaagcggta
     3781 acgcttcatt gaatgctttt gccgtattgg ccgcatcctg accggtcatg gcgtcaacca
     3841 caaacagggt ttcaaccggg ttaatcgacg catggacttg tttgatctcg tccatcatcg
     3901 cttcgtcaac gtgcagacga ccagcggtat ccaccagcag cacgtcgtag aatttcagtt
     3961 tggcttcttt cagcgccgcg ttaacgatat ctaccggctt ctgaccaaca tcagaaggga
     4021 agaaatcaac gcccacctgc tctgccagcg tctcaagctg tttgattgcc gccgggcgat
     4081 aaacgtcggc agaaaccacc agcactttct tcttgtgctt ctcgcgcagg aacttaccga
     4141 gtttaccaac gctggttgtt ttaccggcac cttgcaggcc cgccatcagc acgaccgcag
     4201 gcggttgcgc cgccaggttc agggtctggt tctcttcgcc catcgccgca accagttcgt
     4261 tacggacgat tttgacgaac tcctgccccg gcgtcaggct cttattaact tcatgaccaa
     4321 ccgctttctc ttttacgcga ttgataaact cacgcactac cggcagagcg acatccgcct
     4381 ccagcagcgc catgcgcact tcgcgcagcg tatcttttac gttgtcttca gttaggcgtc
     4441 cacggccact gatattgcgc agcgtgcgcg acaaacgatc ggttaaatta tcaaacattg
     4501 tctctcgcct ggggtggaaa cggttggccg caatcgcgac acatcatcag tattttgccg
     4561 cagtataaca tgaaggcgtc tttgttgtta tgcaacggtt ggagcagcgt tcacctgacg
     4621 ctatactgct tctctttctt attgctcaaa ctgttgacat cactatgccc gtttttgctc
     4681 tgctcgcgct tgtcgcctac tccgtcagtc ttgcgctgat tgttcccggt ctgctgcaaa
     4741 aaaacggcgg ctggcggcgc atggctatta tttctgcggt cattgcgctg gtctgccacg
     4801 caatcgctct ggaagcccgc atcctgcccg acggtgatag cggacaaaac ctcagcctgc
     4861 tgaacgttgg ttcattggtc agtttgatga tctgtacggt aatgaccatt gtggcttctc
     4921 gcaatcgtgg ctggttgctg ctacccattg tctatgcctt tgcgcttatc aacctggcgc
     4981 tggcgacctt catgcccaat gaatacatca cccatctgga agcaacgcct gggatgctgg
     5041 tgcacattgg cttatcgctc ttttcctatg ccacgctaat tatcgccgcc ctgtacgcgc
     5101 tgcaactggc gtggattgat taccaactga agaacaagaa gctggcattt aaccaggaaa
     5161 tgccgccatt gatgagtatc gagcgtaaaa tgttccacat cacgcagatt ggcgtggtgc
     5221 tgctaacgct cacgctttgc actggcctgt tctacatgca caacctattt agcatggaaa
     5281 atatcgacaa ggctgtgctc tctatcgtgg cgtggtttgt ctatattgtg ctgctgtggg
     5341 gacattacca tgaaggatgg cgtggacgcc gcgtcgtctg gtttaacgtt gcgggcgcgg
     5401 taattctgac actggcctac ttcggcagcc gaattgtcca gcagttaatc agctaaaccc
     5461 agaaaaggag tttcccctgg aacacatttc tactactacg ttgatcatta ttctgatcat
     5521 catggtggtc atttcagcct atttttccgg gtccgaaacc ggaatgatga ccctcaaccg
     5581 ctatcgtctg cgacatatgg cgaaacaggg taatcgctcg gccaaacgcg tcgaaaaatt
     5641 gctgcgtaag ccagaccgcc tgataagcct ggtgttaatc ggcaataacc tggtcaatat
     5701 tcttgcctcc gcgctcggca ctattgttgg gatgcgtttg tacggcgatg cgggcgtggc
     5761 aattgcgact ggtgtgctga cttttgtcgt actggtattt gctgaggtat tgccgaaaac
     5821 cattgccgcg ctgtacccgg aaaaagtcgc ttatccgagt agttttctgc tggctccgct
     5881 gcaaattttg atgatgccgc tggtctggtt gctgaatgct atcacccgta tgctgatgcg
     5941 catgatgggt atcaaaaccg atatcgtggt tagcggctct ttgagcaaag aagagttgcg
     6001 cactatcgtg cacgaatcac gctcacaaat ttcccgtcgc aatcaggata tgctgctgtc
     6061 ggtgctcgat ctggaaaaaa tgaccgttga tgacatcatg gtgccgcgca gtgaaattat
     6121 cggtattgat atcaacgatg actggaaatc gattctgcgc caactctccc actcacctca
     6181 cgggcgcatc gtgctctacc gtgattcgct ggacgacgcc atcagtatgc tgcgagtacg
     6241 tgaagcctgg cgattgatgt cggagaaaaa agagttcacc aaagaaacca tgctgcgcgc
     6301 cgcggacgag atctattttg tgccggaagg tacgccgctc agcacgcagt tggtaaagtt
     6361 tcagcgcaac aaaaagaaag tcggcctggt cgtcaacgag tatggagaca ttcaggggct
     6421 ggtgacggtt gaagatattc tggaagagat tgtcggcgat ttcactacgt cgatgtcgcc
     6481 aacacttgcc gaagaggtca cgccgcaaaa cgacggttcg gtgattatcg atggcaccgc
     6541 caacgtgcgg gaaatcaaca aagcctttaa ctggcatcta ccggaagatg atgcccgcac
     6601 ggttaatggc gtcattcttg aggcactgga agagatccct gtcgcaggca cccgcgtgcg
     6661 tattggcgag tacgatatag atattctcga cgtacaggac aatatgatta agcaggtaaa
     6721 agtttttcct gtgaaaccgc tgcgcgagag tgtggcggag taacgaaaac ggccctgcat
     6781 tcgcatgcag ggccgtgaat tattacgaaa acagaaatta cgcttttgct ttcgctacag
     6841 ttaccatcgc cgcacgaatc gtacgaccat tcagcgtata acccttctgc ataatgccca
     6901 gtacgttacc tggcgcaacg tcatcagatt ccaccattgc gatagcctga tgcacattcg
     6961 ggtccagcgg cacgttagtt tcggcgatca cttcaacacc aaacttacgc acaacatcca
     7021 gcatcgactt cagcgtcagc tcaatgcctt caaccatcgc agacatatcc gggttagctt
     7081 tatcagccac ttccagcgca cgatccaggc tatcaatcac cggcagcaat tcgttgatga
     7141 atttctccag cgcaaatttg tgggcttttt caatatccag ttcagtacgt cgacgcaggt
     7201 tttccatttc ggcttttaca cgcaaaatgc cgtcacgttc acgggtctgg gcttcagcca
     7261 gctgagcttc gagattcgca attttttcat cgcgcggatc cacctgctca gcagaagctt
     7321 ctggctcaac tgcctcaatc tcttcgtgct gatccatgat aatttcttcc ggggcttgcc
     7381 cctcaggcgt tttctgttct ttactactca tgaatttctc cgcgtttttt tcgcattcat
     7441 ctcgctaact tcgcttatta tggggatcag tttcagggtt tcaagggaag cactcacatt
     7501 gtcatcaatc ttcgcaacaa ggacctcgga aaaatgaata atcatttcaa gtgtattggc
     7561 attgtgggac acccacggca ccccactgca ctgacaacac atgaaatgct ctaccgctgg
     7621 ctgtgcaaaa aaggttacga ggtcatcgtt gagcaacaaa tcgctcacga actgcaactg
     7681 aagaatgtga aaactggcac gctcgcggag attgggcaac tagctgatct cgcggtagtc
     7741 gttggtggcg acggtaatat gctgggcgcg gcacgcacac tcgcccgtta cgatattaaa
     7801 gttattggaa tcaaccgtgg caacctgggt ttcctgactg accttgaccc cgataacgcc
     7861 cagcaacagt tagccgatgt actggaaggc cactacatca gcgagaaacg ttttttgctg
     7921 gaagcgcaag tctgtcagca agattgccag aaacgcatca gcaccgcgat aaatgaagtg
     7981 gtgcttcatc caggcaaagt ggcacatatg attgagttcg aagtgtatat cgacgagatc
     8041 tttgcgtttt ctcagcgatc tgatggacta attatttcga cgccaacagg ctccaccgcc
     8101 tattccctct ctgcaggcgg tcctattctg accccctctc tggatgcgat taccctggtg
     8161 cccatgttcc cgcatacgtt gtcagcacga ccactggtca taaacagcag cagcacgatc
     8221 cgtctgcgtt tttcgcatcg ccgtaacgac ctggaaatca gttgcgacag ccagatagca
     8281 ctgccgattc aggaaggtga agatgtcctg attcgtcgct gtgattacca tctgaatctg
     8341 attcatccga aagattacag ttatttcaac acattaagca ccaagctcgg ctggtcaaaa
     8401 aaattattct aattttacgc cagcctcttt actgtatata aaaccagttt atactgtacg
     8461 caataacagt aatggttttt catacaggaa aacgactatg ttggcacaac tgaccatcag
     8521 caactttgct atcgttcgtg agcttgagat tgattttcat agcggcatga ccgtaataac
     8581 tggcgagacc ggcgcgggta aatctattgc aatagatgcc ctcggtcttt gtctcggtgg
     8641 tcgcgctgaa gccgacatgg tgcgtaccgg cgctgctcgc gctgacctgt gcgcccgttt
     8701 ttctctgaaa gatacgccag cggctctgcg ctggctggaa gaaaatcagc ttgaagacgg
     8761 gcatgaatgt ttgcttcgtc gcgtaatcag cagcgatggt cgctcccgtg gtttcatcaa
     8821 cggtacagct gttcctctgt cacaactgcg cgaactgggt cagttgctga ttcagatcca
     8881 tggtcagcac gctcatcaat tactcaccaa acctgagcac caaaaattcc tgcttgatgg
     8941 ctatgccaat gaaacctctc tactgcagga aatgaccgca cgttatcagt tgtggcatca
     9001 aagctgccgt gacctcgcgc atcatcaaca gttaagtcag gaacgcgccg cccgtgcgga
     9061 actgctgcaa taccaattaa aagaacttaa cgaatttaat ccgcagcccg gagagtttga
     9121 acaaatcgac gaagagtaca aacgtctggc gaacagcggt caattgctga ccaccagcca
     9181 gaatgcattg gcattaatgg cctacggtga agacgcaaac ctgcaaagtc agctttacac
     9241 ggctaaacaa ctggtgagcg aattgattgg catggacagc aaactgtccg gcgtacttga
     9301 tatgctggaa gaagctacca tccagattgc tgaagccagc gatgaactgc gccactactg
     9361 cgatcgtctg gatctcgatc ccaaccgact atttgaactt gaacagcgca tctcaaaaca
     9421 gatttcgctg gcacgtaaac atcacgtcag ccctgaggca ttgccacagt attaccagtc
     9481 gctactggaa gaacagcagc aactggacga tcaggccgac tcacaagaaa cgcttgcgct
     9541 ggcggtaacg aaacatcatc agcaggcact ggaaatcgcg cgcgcattac accaacaacg
     9601 ccagcaatat gcagaagaac ttgcacagct gatcaccgac agtatgcatg cgctctcaat
     9661 gccgcatggg cagtttacga tcgatgttaa atttgacgag catcacctgg gcgctgacgg
     9721 tgccgatcgt attgagtttc gggtaaccac caacccaggt cagccaatgc agcctattgc
     9781 caaagtcgca tccggtggtg aattgtcccg catcgcactg gcaacccagg tcatcacggc
     9841 gcgtaaaatg gaaaccccgg cactgatttt tgatgaagtg gatgtaggga ttagcggtcc
     9901 aacagcggca gttgtcggca aactgctgcg tcaactcggc gaatcaactc aggtgatgtg
     9961 tgttacccac ctgccacaag tcgcgggatg tggtcatcaa cactattttg tcagcaaaga
    10021 aaccgatggt gcgatgacag aaacgcatat gcaatccctg aataaaaaag cgcggttaca
    10081 agagctggcg cgcctgcttg gtggcagtga agtcacacgt aatacactgg cgaatgcgaa
    10141 agaactgctt gcagcgtaaa cttttttcct gcttcacggt cagagtaaac agcaaaacgc
    10201 cgtaagaccg gaaagcaaaa ggttttaaag tgatgaaagg tctattatca tcggcatatt
    10261 acagatgagc cacgtactgc tagggcccga aaaggaatca aatcactatg cgctgtaaaa
    10321 cgctgactgc tgcagcagca gtactattga tgttgaccgc aggctgttcc actctggagc
    10381 gagtggttta ccgtcctgac atcaaccagg ggaactatct gaccgctaac gacgtatcca
    10441 aaatacgtgt tggcatgacg caacaacaag ttgcgtacgc attgggtaca ccgctgatgt
    10501 ccgatccatt tggtacgaat acctggttct atgtcttccg ccagcaacca ggtcatgaag
    10561 gtgtaacgca gcaaacgctg acgctgacct ttaacagtag cggtgtgttg accaatattg
    10621 ataacaaacc tgcgctgagt ggtaactaat aataaagttg ctctcaaaga cgttaaaaaa
    10681 ggtgctcaat gagcaccttt tttctgtctg ttatttattc gctgattttt ctgctcgttg
    10741 cctgcgaagc tctttcggat cggcaatgag agggcgataa atctccaccc gatcgccatc
    10801 atgcacacta tcgcttagtt ttgccggacg gctgtaaatg ccgactttgt ttttagttaa
    10861 atcgatatcg gtacgcaatt ccagcaagcc actggcgcga atagcttctt caaccgtcgc
    10921 gccctcctgc agcgtcacgc gctgcaggta ctgcttctca ggtagcgcat aagccacctc
    10981 aacggcaatt ttacctggca ctgtaaacct ctttcgcacg aaccgtaaaa gcctggacca
    11041 tattagacgc cagctcttta aacacgcgac caaaggcgag ttcaatcaac ttattggtaa
    11101 actcaaagtc gagatgaaac tcgatacgac acgcctcctg gctcagcggc gtaaacttcc
    11161 atccaccaat caatttcttg aacggcccat ccaccagatt cataagaata ctttggttac
    11221 tggtcaactg gttgcgggta gtaaacgttt tgctgatccc agccttagag acatctaccg
    11281 ccgcagtcat ctgcccagga gtggactcca gaatccgact tccggtacaa cccggcaaaa
    11341 actgaggata agactgaacg tcattcacta actgatacat ttgctccgcg ctgtagggta
    11401 ccagtgcggt ccggctaatc tgaggcataa ctatttccat caacaaaaat ccaacaaata
    11461 atatcattta tcctgctgta aaaaaaacgc tatcccggcg ctgggtaaca tcgggttcat
    11521 gctaagatag agccttgtcc cccgcaggat tgatatgggg tgttttcgat ttcagattac
    11581 cgatgattca cgacgcttat gacgaagaaa aaagcacata aacctggttc agcgaccatc
    11641 gcgcttaaca agcgcgcccg tcacgaatac tttatcgaag aagagttcga agcgggactt
    11701 gccctgcaag gctgggaagt taaatccctg cgcgcaggaa aagccaatat cagcgacagc
    11761 tacgtccttc tgcgtgacgg agaggcattt ctgtttggcg ctaacatcac gccaatggcc
    11821 gtggcctcca cgcatgtggt gtgcgatcct acccgtaccc gcaagttact tctcaaccag
    11881 cgcgaactgg actcattgta cggtcgcgtc aatcgagaag gctataccgt agtggcgctc
    11941 tccctgtact ggaaaaatgc ctggtgcaaa gtgaaaatcg gcgtcgccaa aggtaaaaaa
    12001 cagcacgata aacgttcaga tatcaaagaa cgcgaatggc aggtggataa agcacgtatc
    12061 atgaaaaacg cccaccgtta aacctgcact ccaattattg accagttcct caccgcgcct
    12121 ccctctccgg cggcgcgaat gaacatctta ttggctatca catccgacac aaatgttgcc
    12181 atcccattgc ttaatcgaat aaaaatcagg ctacatgggt gctaaatctt taacgataac
    12241 gccattgagg ctggtcatgg cgctcataaa tctggtatac ttgccgttac acattggggc
    12301 tgattctgga ttcgacggga tttgcgaaac ccaaggtgca tgccgagggg cggttggcct
    12361 cgtaaaaagc cgcaaaaaat agtcgcaaac gacgaaaact acgctttagc agcttaataa
    12421 cctgcttaga gccctctctc cctagcctcc gctcttagga cggggatcaa gagaggtcaa
    12481 acccaaaaga gatcgcgtgg aagccctgcc tggggttgaa gcgttaaaac ttaatcaggc
    12541 tagtttgtta gtggcgtgtc cgtccgcagc tggcaagcga atgtaaagac tgactaagca
    12601 tgtagtaccg aggacgtagg aatttcggac gcgggttcaa ctcccgccag ctccaccaat
    12661 catgattgga cggtataagg acaacaccaa taaaaacagg aggttagcag tctcagcagg
    12721 acaccgacca gacggtgaag agacataaaa ggatacgcaa aggagccgcg gctcctggtg
    12781 acatgaaagc ccacagatgt gggctttttc gttgatggtc agaacgacca gttcacacca
    12841 gctaccccgt tccacgggga ttccacaccg gcaccatggc tataccccac cccaagatgc
    12901 ccgcttaacg tactgctgaa tgaggcttta atacctgcct ggtatattcc acgtctgccc
    12961 gacaaatcat tgacgaaatt accgtcacta ttcactttca cccggttatc atcgacaaat
    13021 tctttgcgca cagccgcctt cagccacggc tcaacttcca taccgttccc cagacgcatg
    13081 ttgtaactca gcgttgcacc cagttcacga tataaacctg cgggtatcga ctgatttcga
    13141 ttccatgcca ttggataaat gatattcagg gttatcagcg gtgaaccccc gttaacgatg
    13201 catacggcgt caggttccag ttaccatcgg taaatcgcat cccggtttca atgtgaccgc
    13261 ccagcccgtt gctgcgataa ctgccgttgg tggctccacc gctgccaacc gtggagtcgc
    13321 gagcttcggt acctgctaat accaataaat taccgccatt ttccagcaac atattggtcg
    13381 ctaaattgcc ggaaatggaa aaagtgccgt actggtaagt accgctgatt tcaataccgt
    13441 tagccgtgct cgtctggaga gcggcaccgc tgttctggac gatatctgtc gctttgccat
    13501 tatcgttaac tgtcagcgta ccgccttcat tgatctttgt ttttattgcc tctccgttag
    13561 ctgaaactgt ttgtattccg ccgtcgttaa tcgttgtctc attcgccacc ccctcgacga
    13621 tttgttcgcc gccggtgagc gtcgtgcctg tcgcagtggc ttttgttttg acgatctccc
    13681 gtccgcccgt attgacctgt gttttgtcag aagaggtgtc tgactccacg gttaacacgc
    13741 cgccatttgc cagcaggata ttgttcgccg caccctgctc gatgctgaac gcgacgccat
    13801 ccgcgcgtgt tcctgtgacc cgcgtcgccc tggtggttgc aaccaaagcg ccctggctac
    13861 tctgctgtat ccccgttgcg ctgcctttct cccgcacatc gagtgtgccg ccgtcattaa
    13921 gcaccgagtt ttcagccaga ccgccctcat taactacctg tgaaccccca ttaataatgg
    13981 aaccttccgc tgtcccgttt gccataattt gttgtaggcc agagacgata tcggtattga
    14041 ttgccttacc ataattctga acggtttgcg tgccaccatt gatgtgtgtt ttctctgttg
    14101 acccaccatc aacaatttgt tcaccacttt cgatatttgc ctcagtggct aatccatata
    14161 ccgtttgctt gccacctttg atatttgctt tatcagaagt ggcactggca tatattgttt
    14221 gggtgccagc actattgagt acagtgccaa catcttttcc ataaacatcc attttgccgt
    14281 tggcattaat aatcgtatca actgcccggg aaccagtgac gactgttaat gagccagcgt
    14341 tttccagcac tacattttta gcttctgaat tcctgatgta gaaagcatca ccataactgt
    14401 tggttccttt gataagggtt ccggaagttg tggaagcaat taatgcgccg ccggattgtt
    14461 gctcaacatg cttagcctca ccaccgtcct gaacctccag aacgccacca ttattaagtc
    14521 tggtggtatc tgttttagcc tccttctgga caatcag
//
LOCUS       DAJTSL010000096        14549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTSL010000096 DAJTSL010000000
VERSION     DAJTSL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.096"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO433_002648"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO433_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6051070.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO433_002649"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO433_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6051071.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO433_002650"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO433_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6051072.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO433_002651"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO433_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6051073.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO433_002652"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO433_002652"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6051074.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO433_002653"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO433_002653"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6051075.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO433_002654"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO433_002654"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6051076.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYRGGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO433_002655"
     CDS             complement(4598..5320)
                     /locus_tag="OO433_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6051077.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO433_002656"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO433_002656"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6051078.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO433_002657"
     CDS             6520..6738
                     /locus_tag="OO433_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6051079.1"
                     /translation="MQDLSLEARLAELESRLAFQESTIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO433_002658"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO433_002658"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6051080.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO433_002659"
     CDS             complement(7472..7672)
                     /locus_tag="OO433_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6051081.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OO433_002660"
     CDS             complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OO433_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR6051082.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEE
                     DEKPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYY
                     GDATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHE
                     LLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHA
                     DTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO433_002661"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO433_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6051083.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELANHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OO433_002662"
     CDS             10175..12088
                     /locus_tag="OO433_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6051084.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALSQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OO433_002663"
     CDS             12088..13110
                     /locus_tag="OO433_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6051085.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVIFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OO433_002664"
     CDS             13104..13322
                     /locus_tag="OO433_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6051086.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OO433_002665"
     CDS             13376..14245
                     /locus_tag="OO433_002665"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6051087.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..>14549)
                     /locus_tag="OO433_002666"
     CDS             complement(14300..>14549)
                     /locus_tag="OO433_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6051088.1"
                     /translation="VSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDL
                     KDAAVARAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
BASE COUNT         3597 a         3967 c         3582 g         3403 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcagt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgattgtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt taatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccgga ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt cacctatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc cctccccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggtgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaagcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtgacc atgagccagt tcttcttcgg tcgcttcacg
     6961 aatcgccaca acctcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggcc cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccaag tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaat agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggataa ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ttcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ctgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccggcgt tatcgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc aaggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcaacaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcaag cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gcgacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtgatatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc cctcgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atattatgcc gcgcagtcag tttcgcgaat
    13321 aacctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga agcgaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg aacattcacc ccctggcaac ctttaccaga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtagtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctg gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcggc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atcgaaacc
//
LOCUS       DAJTSL010000097        14523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTSL010000097 DAJTSL010000000
VERSION     DAJTSL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.097"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1264)
                     /locus_tag="OO433_002667"
     CDS             complement(<1..1264)
                     /locus_tag="OO433_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR6051089.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQVAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNSPALTGTPTTPAARQGTNNTQIASTAFVMAAI
                     AALVDSSPDALNTLNELAAALGNAPNFATTMTNALAGKQPKDATLTALAGLATAADRF
                     PYFTGNDVASLATLTKVGRDILAKSTVAA"
     gene            complement(1267..1797)
                     /locus_tag="OO433_002668"
     CDS             complement(1267..1797)
                     /locus_tag="OO433_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCR6051090.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPGD"
     gene            complement(1790..2686)
                     /locus_tag="OO433_002669"
     CDS             complement(1790..2686)
                     /locus_tag="OO433_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6051091.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCASFTISEGGRNE"
     gene            complement(2690..3019)
                     /locus_tag="OO433_002670"
     CDS             complement(2690..3019)
                     /locus_tag="OO433_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCR6051092.1"
                     /translation="MNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            complement(3037..3603)
                     /locus_tag="OO433_002671"
     CDS             complement(3037..3603)
                     /locus_tag="OO433_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR6051093.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTSRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(3615..4250)
                     /locus_tag="OO433_002672"
     CDS             complement(3615..4250)
                     /locus_tag="OO433_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR6051094.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     PQKAPDGTAWTPRRRHVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNS
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(4243..4710)
                     /locus_tag="OO433_002673"
     CDS             complement(4243..4710)
                     /locus_tag="OO433_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6051095.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLLEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(4848..5255)
                     /locus_tag="OO433_002674"
     CDS             complement(4848..5255)
                     /locus_tag="OO433_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCR6051096.1"
                     /translation="MKLWPTLGVAFLLTAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            complement(5252..5644)
                     /locus_tag="OO433_002675"
     CDS             complement(5252..5644)
                     /locus_tag="OO433_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR6051097.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQMSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(5641..5964)
                     /locus_tag="OO433_002676"
     CDS             complement(5641..5964)
                     /locus_tag="OO433_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6051098.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(5967..6167)
                     /locus_tag="OO433_002677"
     CDS             complement(5967..6167)
                     /locus_tag="OO433_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCR6051099.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(6167..6661)
                     /locus_tag="OO433_002678"
     CDS             complement(6167..6661)
                     /locus_tag="OO433_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCR6051100.1"
                     /translation="MTIVARTEPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(6829..7575)
                     /locus_tag="OO433_002679"
     CDS             complement(6829..7575)
                     /locus_tag="OO433_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR6051101.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKKQQK"
     gene            complement(7621..8493)
                     /locus_tag="OO433_002680"
     CDS             complement(7621..8493)
                     /locus_tag="OO433_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCR6051102.1"
                     /translation="MIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLKNR
                     KYQCEQVNYDTFISYPQLDAWSAHPDFQSRISAQIARQVALDRIMIGFNGTSHADESN
                     FSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQDAR
                     SSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIGGL
                     GVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVEDY
                     GKCAMVTGLKFADS"
     gene            complement(8490..8672)
                     /locus_tag="OO433_002681"
     CDS             complement(8490..8672)
                     /locus_tag="OO433_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCR6051103.1"
                     /translation="MNLVMSDITRNKLGCYMAQQVSLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKKIT"
     gene            complement(8696..9532)
                     /locus_tag="OO433_002682"
                     /pseudo
     CDS             complement(8696..9532)
                     /locus_tag="OO433_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
     gene            9687..11438
                     /locus_tag="OO433_002683"
     CDS             9687..11438
                     /locus_tag="OO433_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCR6051104.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPGQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPSVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            11438..12474
                     /locus_tag="OO433_002684"
                     /pseudo
     CDS             11438..12474
                     /locus_tag="OO433_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(12953..13543)
                     /locus_tag="OO433_002685"
     CDS             complement(12953..13543)
                     /locus_tag="OO433_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR6051105.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADKHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKHAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNCGWFSPLTTDKNNT"
     gene            complement(13607..13918)
                     /locus_tag="OO433_002686"
     CDS             complement(13607..13918)
                     /locus_tag="OO433_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6051106.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            complement(13923..>14523)
                     /locus_tag="OO433_002687"
     CDS             complement(13923..>14523)
                     /locus_tag="OO433_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR6051107.1"
                     /translation="KKANVMRPVEYQNGEAFTIRNVQVMPESIPAGFKALADMSPFES
                     LLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSHHIAN
                     TIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVMVVGG
                     GAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
BASE COUNT         2950 a         4046 c         4003 g         3524 t
ORIGIN      
        1 cggcagcaac ggtcgatttc gcaagaatat cccgcccgac ttttgtcagg gttgccaggc
       61 tggcgacatc attccccgta aaatacggaa acctgtctgc cgcagtagca agcccggcca
      121 gcgccgtcag ggtggcatct ttcggttgct tacccgcaag cgcgttagtc atggtggtcg
      181 caaaattcgg ggcgtttccc agcgccgccg ctaactcgtt cagcgtattc agtgcgtcag
      241 gtgacgaatc tacaagggcg gcaatcgcag ccataacgaa agccgtgctt gcgatttggg
      301 tattattcgt tccctgtcgc gcagctggcg ttgttggcgt tccggtcagc gcaggactgt
      361 ttaagggcgc tttcttgttc gtttcatcca ttaccgcctt aaccgctttc gatgtcgcgg
      421 ccagtgtttc agacgtgctg ttggtggcac tactgagctg gactatccct ttttgtgccg
      481 tcgtggcgtc ctgagcggta tattttgcgt tagcaaggtc atacgcggcc tttactgctt
      541 tcggcgttgc agcctgcgtt tcagacgtgc tgttggtggc gctactgagc tggacaaggc
      601 cttttcgcgt cgtgctggca tcctgcgcag tatatttacc gttagcaaga tcatacgcgg
      661 cctttactgc tttcggcgtt gcagcctgcg tttcagacac gctgttagtg gcgctactga
      721 gttgaacaaa gccttttgcg gtcagcgagg cgtccgggtg acgtcgtgac tgttcatgct
      781 ctttcagttt gtcatccacg taatccactg tggccatcac catggtgttg tccacggtaa
      841 gcgccacggt ggcagtgctg gatacggtca gaatggtgcg aaatgtttgt gcacgaccgg
      901 acccttcggc aacggctggc ttgtaacttt cggcagtatt tcccaccgcg attaaatcgc
      961 cgtgctcatc aaatacacca atttcccgga tccagaatcc gcccgtttca ggaggaataa
     1021 ccagctccgc aataatgcgg ttctgatgtg ttgcgtccag gatgacgcga ttaacagtat
     1081 gtcgccacac ctcatgcacc agacgggtct gcttactgtc tggtgtgggc aacgtgccgc
     1141 caccgtcgcc cacggccata tgagtcaggc ggacaggctt accatctggc gcggctacct
     1201 gagctaattt ttttgcaccc gtatcggtga taatggtttt aaattttcgt gttgtggtac
     1261 tcatgcttaa tcgcctggat aaatggtaat aacttcaccg tcataagttg ccgccgctgc
     1321 gaaaatatcc cccggaattt cctgaatgat attcagccct gtcatgtggc ggctgaccgg
     1381 gcgggcatca gcaatcaacc gctccatttc cagatacatt tcctccgtca cgccactgtc
     1441 cagcgtgccg acttcaacgg taaatgttcc cggttctccg ccgaactccc accactcaga
     1501 cacgcgaatg aggtatccca gcggctcaat ggcccggcgc agtgcgctga tggtcccttt
     1561 gtgtcggtgt atcagccatg catcacgaat aacctgtcgc tttgtctctt ccggccagtt
     1621 gcgatcccag cggtcaacgg aaaacgccca ggcgagataa ggcagcagat gcaccgggca
     1681 ggtgtccggc gaccacagcg tgttgaggtc taccggaatg tctgtaatgc gtgttccgac
     1741 ggcttcggca caacgcatga aattgctggc tgatggtggt aacaacgaat tactcattgc
     1801 gtccaccttc gctgatggtg aatgacgcac agcgcgccgc ctgtatgtcg ctgatggcca
     1861 tattctgtgt gggttcgatt atctccacgc gttgcacacc gtgcacatgc agtgcggcag
     1921 caatggcgga caacgccacg tcctgaccga taagcccctg ctcagccagc cacttcctga
     1981 acgacgattc agccgcggcc agaataggtt cggattccgg gccggggtaa aagtacagtt
     2041 ttgcattcag ccgccatgtc acgattctgg cactctgtac ggtcaggcgg tcggccaccg
     2101 ggcgggtatc ctctgcattc agaacggcgc gaacggtatt aagcaacgcc tccgttgctg
     2161 tgccgtcgcc ctcagtggac aggatggaaa ccgtcacgtt ggccggagac gggctgatgg
     2221 cccgcgcatc gcgtaccaga ccgctggcgc tgcgtgcaaa atactcgtat gcacctgacg
     2281 ggccagcaac actcagaccg tcatatgccc gttgcgcccg tagtctcagc gaggtgtcac
     2341 tttccatcac tgcatcggtg gtatccgttg ccggagtgat aaccaggcgc tttgtgttca
     2401 tattgcccgc gaggttgtcc aggtctgtcc cggcgctgtg gcttagcatg caggcgcgtg
     2461 caccctcatt gacccgctgg cgtaacagca tttcacgaaa cgacatggtt tgagcgataa
     2521 cgttaagggg ttccgattcc agctccagcg cggcggaaac ggcttcacgc tgttcggcgg
     2581 gataggacgc aatcatcatg gcctttgtgt cagccagaat tgcctcaaag tcaggctccg
     2641 cgatgatggc gggttccggt aactgtgaaa ggtcaacggc aggcatgatt tactccctca
     2701 gcgtgatggt taattcaaca ttctgcatgg tctgcatgac agtgcccgac agcgtcaccc
     2761 cggcgcggcc tcctgccttc cagacaacgt cgatagcgtc cagggcaatg cggggttccc
     2821 atcgtgtcag cgcaatcacg gcagcactca tgcattgcag acgcgtggtg ttattcatgg
     2881 gttcgtcaat caaatcaggc acaaggctgc catattcccg tcgcataacc cggcttgcca
     2941 gcggggtggt caggatgtcc ctgactgact gtttcaggtg ctccatatcg ttcaggtttc
     3001 ccgttccgtc cgggttcatt cctgtgtagc gggttgtcac tgcgggcctc ctgtcgaatc
     3061 gctgccacct ttaacgccac cgtgtttatg cgtatgcact gtgatgccgt ttgaggtgaa
     3121 attaccgccg ctgtgcgtga tattgccgct catctttccc ccttttgtga cgtcaagcgt
     3181 cgccgttctc agaaggtctg tgcattccac gacgggcgta tccagtgtca cgctgacgga
     3241 tgcctgcagg gtggctgttt tcatgccgct ggcgctcagt gcgcctgcgt ccgcgtcgta
     3301 gcggaacacc gcgccatccg gcgcgctgac cacgatttct ttcaggcttt tgccgggggc
     3361 cggaatggca tcactccaca ggctgccaat tatcatggcg gtttccgggt tgccgccaat
     3421 gcaggcaatt accacctgtt cgcctggtga tggcggcagc cacacattga aggctcccgc
     3481 gcgagaggtg ttccagcgca gccaacctgt ttccagttcg ccgctgcgaa cgcgcacgcg
     3541 ccaggacttc tcatcaactt cagagatgat cccggtgcgg atgatattgc tcagtagccg
     3601 catgagttct gcgctcaccg tacagcctcc gcaatccggc ccagcaccgt gttataaatc
     3661 aggcgctcat ctgcctggct gatacccagc agctcacgta ccgggtaatc ggtgaaaatg
     3721 cccggcgcaa cctgatcgcg ctcaccgaac tgatgaacgc gtgcaatacg tgcggccacg
     3781 ccgctgtaac ccaccgtcac accggaagca tctgcacggg ctttcaggta gcgggcggtg
     3841 cgcagtttta cgaacatggg gacgcgcttt gtgctgtcct ggttgatgcg ccgggtgcgt
     3901 atttccagaa agcggtcgat gtcatcccgg taaaacgtgc ggatgctgtt tttatcctca
     3961 tcccacccgg tgatggttcg cccgtatttc cccgtgtcgt gatgccagtt tttcagcgtg
     4021 cgtgcttcgt tattccagat aaagcgaatg cgctcctgta tccgggttac gtggcgtctg
     4081 cgtggtgtcc atgcggtccc gtccggcgct ttctgtggcc ggatacgtgc ctgctgggcg
     4141 cggcgtaaat cctgtgccag ctttctggcg atgttattga tggcctgctg attcaggctg
     4201 tcgcggatgg cctcaaaggt ttcatccacg cgagtgaatg ccttatccat cgctttcacc
     4261 ccacgtcaca tcctggaata catgcgacca gtcgccttcg gaagatggca ggcggggttt
     4321 tggctccagc aggtgttctg cctgcggtgt gccctgactg ctgcgcgtga tgcgaacgcg
     4381 ttcccgcagg gggagcgtaa acaggagatc ggcgctgtca tcgtcattga taacggcgga
     4441 aaatttgatg tcctgattac gctcagggtt gagcaacaac tgtggctgat tttcggataa
     4501 ccacgccagc agcggcagcg tgaggtcgtc cagctccccg gcgtaatcca tgacaaacat
     4561 caccatctga tagcggtaaa caaacgatgg ggtttctccg gtcgtttcaa tgttgccgct
     4621 ctccacgaaa atggtgaatt tttccgggtt agcctgacac catcggcatg aacgggtcat
     4681 ggcttcacgc agggaatcag ttttcagcat ggttgttatc ctcgttgttc agtcgttgca
     4741 gcctgcgctg ttccagtaat tcaatggccc gtttatccgc gttacaggtt tccagtgcat
     4801 ccagaaggcg gtcgccccat ataccgagat ttccccatgt gggagtgtca gggaaggggg
     4861 gaggcgttac cggtatggtc agcgtctgcg gtataagccg gactgacggc gctggcagtg
     4921 gcgcgttctg cgtgcctgcg cagcctgtca gtaaaacgag cgtcaggcaa agcgtgggcg
     4981 cattcatctt ttgcaatatc gttgcgtagc tgttcacgtc ttacctctcc gtcctgattg
     5041 cgttgctgat tttccacgcg gagttgcgcc agcacctgct gcatatcctg taccccggcg
     5101 ctgatgatat tcagggtgtc ggcggtactt ttcagggtgc tggcctgcgc ttcgtttctg
     5161 gcgttctccc ggcccagcga ccacgacaga cgcatggatg ttccccatcc ggcagtcaga
     5221 aggaaagcga cgccaagcgt gggccagagc ttcatgccgg ataggctccg tgtggtaact
     5281 gaaaatgcgg tccgtctttc agggtcttcc agtcgccgcc ccattccacc ggaatattca
     5341 gttcccggct ggcctgtctg aatgctgctg cgattttttc gtacagcggc cattcccatg
     5401 acatctggct gccgatataa gccacaacat ccacggcatg ccccgtaagg tggcggctgt
     5461 tcatggtctg gctcttaccc gtggccacca gttgcttctg gcggtaacgg ctgcgcaacc
     5521 cttcggtgat accaaaatcc acttccgaga tttccagtgc ccgtcgggtc actttcacca
     5581 gatcaggatt tacaccctgc aaattctttt cgctccggct gctgaattta aatgtgttgc
     5641 tcattcgtcc ttctccttca ccctgcgatt aaaggccgca ataaccttgt cgcgtgcttt
     5701 ctctgcaccc ataaaaccga ttgatgcgcc gataaacgtc acggcatctt caggaaaccc
     5761 gaagaagcgc aacgacccgg ccacggccat ggcaagaacg ccgcacgcca gcgatcccgt
     5821 tacggtctga accagtgttc gtccgtcata aaggctcatc agcgcggaaa tgctgaccgc
     5881 cgcgcctact gcatacaccg ttggcaggtg gtcaaagagc cacgcaataa cctgctctgt
     5941 gatccctgtt tgaatggtgc tcactgctac tccccccaca actgaatcat ttctcgtttc
     6001 ttcttctccg gctccggcat ctccacttcc tgcccggcgt ccagaaatac ctgctgacag
     6061 agtccggggt tggcatccag caccttttcg gtgacgccct gcgtcgtgcc gtagtaccgg
     6121 aaacagagcg aatccacggt gtcgccttcc agtgccttca ctttcatcag cacaactccg
     6181 caaagattcg cgggcggcac agaatgtcag agatggccca gctcacatca cgccacaaat
     6241 ccgatgtctg tatatccaga gcgtctgccc ggcggtcgcc cttgtccgta gtgtctgcat
     6301 cacgataacg ctccagaatc agggcgcgcg tggcggtata aacagcattg cgccagtgcc
     6361 agagattgac gctttctccg ttaattacgg gtgccggaac atcggccagc gtctgatggc
     6421 cagctgcctg ctgttcctgc tgccatgctt ccagctcgcg ggtaacgtgt gccacagccc
     6481 cggtggcagt atgcagcagg cgggaggtgg tcacgcgccc cggcagtcgt accgccagac
     6541 gcagctcgcg cagcacaata tccggccaga atgcacccgc tgaaatgcgg gtatcgccat
     6601 catcggtatc ggtgatgtcg tcctctgcgg gtccgggttc agttctggca actatagtca
     6661 tggggttcac tcctgaaaaa aaatcgggcg gtgggtgcgc ggtgtaaacg gtcacggagt
     6721 caaactggaa caccgcgcac gccgcccgct gacggggtca gtcgttaacc gcgcttcgcc
     6781 ttctgcgtcg cggtggtttt tcgtgttgca ggcttccgcg ttgtcttttt acttttgctg
     6841 ctttttgctg ctttcgtcct gcgcctgctg tgcgctggcg tcttctggtt cggctgcgga
     6901 atcggctttt ttcagggcgc gggaaagggt tgcaatctcg cgtttcacac ctgcgttcgg
     6961 gttcaggtgc attgcttcgc gcagcagctt cagtgacagg gccatgctgt ccgcatcact
     7021 caggccacgg cgggcaaagg cgcacgcttt gcataatttg gcgcgcactt cgtccggcat
     7081 gtcctggtcg gtgacaatct cccggagggt gtccagtggt tcgataaagg cggacaaatc
     7141 cgcgtcggca tccgtcccgg cctgcgtcag taccggatta cagatttctt cggtcagtac
     7201 cgtggcagca gtacggccaa agttatccgg catgatgagg ttgtgacgga ctacatacgc
     7261 gccgatacgc agcgcaagcg gaagatcgcc acagtcaatc gcccacacca tcagcgtggc
     7321 aatcacttca tcctgctgcc cgccgtcagc ctccagcgtt ccctcaatcc agccggaaaa
     7381 atccggcaac aactcttttt tgatggcggc tttcgcgctt ctggcctgta cgcccttaag
     7441 ccgggcctgt gccagacgca gacgatacag cacctcttca tgcgcggtac gcgcggcgtg
     7501 gtccacgcct tcattcgccc ggcctgcgcg ctgtgccatc acgttctgcc agtgttgttg
     7561 tgcaggagta atcatttttg ctctccgtta caggcgggca tgatgcccgc cgtgagttga
     7621 ttagctgtcg gcgaacttca ggccagtaac catcgcgcac ttgccatagt cttcaacgac
     7681 ataagcgtca ttgatggact ggtaggtggc gatgcggttg tattccggct cgtctttcat
     7741 caggcgacgc attgaacctt tctgccagta aatcgacagg ttgttgaacg aggtgatcag
     7801 catcgttgca tccgggaaga acggcgcaag gaacacgccc agcccgccaa tggtgcgcga
     7861 tgacaggatg agctgcccgg cgagtaattc cgcattggga ttctggccgc tgatgctgtt
     7921 cagcacgggc agacgcagcg agttaaacag gttgcgcccc ataatcacca cgaggtcgtc
     7981 agcttccttg tgccattcat ctagcaggga tgaacgcgcg tcctgtacca gtgcatcagc
     8041 gttcgcatac ttacccgcgt gcgccacggt gttgtccatg ttgcgggagg tcagcgtcac
     8101 gtcattcata acgcgttcgc tggcgtcggt tctgatgtgc tccagccagc ccacgttaac
     8161 gtcctgaagc agcttgttgg tgctgaagtt ggactcatcc gcgtgagacg tgccgttgaa
     8221 accgatcatg atgcggtcaa gcgccacctg tcgggcaatc tgtgcgctga tgcgggactg
     8281 aaaatcaggg tgtgccgacc aggcatcaag ctgcggatac gaaataaacg tgtcgtagtt
     8341 cacctgttca cactgatatt tgcggttttt cagatcaacc acgttattcg ggttacggcg
     8401 ttttgtgccg tcataactgg tattcgtgcg cgcaatcggt cctgtggtat ccaggaggat
     8461 tttttcgcct ttctggtcgg tcacgccgat cacgttattt ttttcgtaaa ttcggtactt
     8521 tcctttgagg cgttttcaaa acgctgctgc accgcgggtt ccacggtaaa tcgcgacacc
     8581 agtgcagata ccgggatatt attaagcgac acctgctgcg ccatatagca acccagcttg
     8641 ttgcgggtaa tatctgacat caccagattc ataaaaaatt tgctcctttg tctaatcaga
     8701 agtcggccag ctggtcggag gctgcgcccg ttgcggtgaa gcggttctgc ggatcgccgt
     8761 cctgcgtgcg cagtttttcc ttcagtgctg ccagctctgt ggtcagtgac gtaattttct
     8821 ggcggtcctg ctgatggcgg gtttccagca cattaaaacg gtcgataatg tcggcctgtg
     8881 acgttgcgac gccttccacc gcttcctgaa tacgggaaaa actggcgtca tccgctttgc
     8941 ggccacggcc aataatcccc attacgcggt taaaccactg ggtgccttct tcctggcgtt
     9001 gttctgccat ttcgatgatt tcagactcga tggcttcgga gataagcggc gcttcaccct
     9061 ggatactgtt aaacgtcatc accgcctaac gttgctgtgc cgtgaatttc aggcgctcag
     9121 tgcccaggct tgccggggtg tcggtcatcg ccagcccgac cagataggcg cgcccgttaa
     9181 cggagaactg cgggtgcagt tcgatactgg aatagatttt cttgccgtcc gcaacaagct
     9241 gcttcatgcg ctcggtcggt tcgatttctg catacagcgc agtacgtccg gccagcggac
     9301 cttccgtaat gtcttccgta ctcagtgcgg tgacatcgcc cattgcggaa aattcgcttg
     9361 acgggcatgg cgagagatag tgctcaacgt tcacgcgggc agcgtaaaca tccgggttga
     9421 agttctcggc ggcttcacgc agatgcaccg gactgatttc acggccatca acagttgatc
     9481 cggagacagc cacgcgaaac tttttgcggg atgtcttttt ttcattagcc atagtttttg
     9541 cccctctgac tggttcttca gtcatgatgg caaagcgtaa caggctgata caaagggctt
     9601 ttgttgtaag aaaacggcca gaacagaggg ttaaggagaa cagtttcgcg cgcgggtaat
     9661 cttcctgtaa ttactcaggg ggagcaatga ttcaggacgc ttttgtgcgc cagcgtgcgc
     9721 ggcaacttta ctggcagggt tatccgcccg cagaaatatc acgtctgatg ggaataaacc
     9781 cgaacacgat ttatgcgtgg aaaaaacgtg accagtggga tgaaacgcca cccgggcagc
     9841 gtgtcacgca gtccatcgat gcgcgcctca tccagcttac tgaaaaacag aataaaacag
     9901 gcggtgactt taaggaaata gacctgctga cccggcagct taaaaagctg catgatggcc
     9961 agccggatgt gatggccgca ggaaagaaag gccgggcgaa aaaactcaaa aatcatttca
    10021 cgccggaaca gattgccgca ctgcgggaaa aaatcatcag caggctggag tggcatcagc
    10081 ggggctggtt tgactccctg accctttgca gggaagccgg gatacgtaac aggatgatcc
    10141 tgaaatcccg acagattggg gcgacctggt attttgcaca ggaagcactg ctgatggcgc
    10201 tgcgtgacga tgtggcgcaa ccttaccagc gtaaccagat ttttttgtct gcgtcgcgtc
    10261 gtcaggcgtt ccagtttaaa agcattattc agaaggccgc ggctgaagtt gatgtggagc
    10321 tgaaaggggg cgataaaatc atcctctcca acggcgcaga gctgcatttt ctcggcactt
    10381 ctgctgcgtc ggcacagtcc tatacgggca atttttattt tgatgaattt ttctgggtca
    10441 gtcgctttgc tgaactgcgc aaggtggctg gcgctatggc aaccctcagc ggactgcggc
    10501 gcacctactt ctccacgcca tccaccgaaa cgcacgaggc atacgcctac tggaacggcg
    10561 accgctggaa cgagaaaaag gcctcgcata aacgccagcg tttttctgtg gactggaaaa
    10621 cgctgcataa cgggcttatc tgccctgacc ggacgtggcg gcaaattgtc acgctggaag
    10681 atgtggttaa tcacggctgg aaacacaccg atatcgacga aattcgtgat gaaaacaccg
    10741 aagacgagtt cctcaatctc tatatgtgtg agtttgtccg cgaaggggaa tcggcattta
    10801 acctgaatat cctgattggc tgcggcgttg acggatacga cgactggaaa gactggaaac
    10861 cttttgctcc ccgcccgatg gggaatcgtc cggtatggat tgggtatgac gcaaacggca
    10921 gcagtggcaa cggcgacagc ggcgctgtgt ccgtggtggt tcctccgtct gttcctggtg
    10981 gccgttttcg aacggtggag acgcgacgcg ttcaggggct ggagtttgaa gaacaggcca
    11041 gagtcattga agagttcacg tgtcgctaca acgtggaaca catcggcatt gatgtgacgg
    11101 gcgggaacgg ggaggctgtt tatcagatag tgaaacggtt tttccctgcc gctattccgt
    11161 acaccttcac gctgtcatca aaacggtcgc tggtactgaa aatgctgcaa ataatgcgtg
    11221 ccgggcggtg ggaatacgat cgcgccgaac gcgagctggt cgcggccttt aacgccgtgc
    11281 gtaaggtgaa aacaccgggc ggctttatca cttacgaaac ggaccgagcg agggggatca
    11341 gccacggcga ccttgcgtgg gcaaccatgc ttgctgtcat taacgaaccg attggcggcg
    11401 aaggagaaaa cgagcgtttc acggttatgg agttctgatg agcagaaaaa ataaaaaagt
    11461 gcgcatgagt tcacgcattg atctcgctga tgcgctcagg aaagaatcat cgctcagtgc
    11521 attcacattt gatggtcctt atcgcctgac ctgctggaca atatgtactg tgctgataac
    11581 gggcggtggt atgaaacccc ggtggactgg tacggtctgg caagagctgc ccggcaaacg
    11641 tcctggcatc agtctgcgct ttattttaag cgcaatgtat tgctcggttg ctacatcccg
    11701 cacccgctgc tttcccggca ggatttctcg gcgctggcgc tggactggtt tgtgttcggt
    11761 aacgcattcc ttgagcttcg aagcaatatg ctcggcgaac cgcttaaatt acggcacgcc
    11821 ctggcgaaat acatgcgacg cggaagcgat cttgaatcat ggaggtatgt gcaggatggc
    11881 aaggatgcgt ttcagtttcg cccaggcaaa gtgtgtcacc tgatgaatcc tgacattaac
    11941 caggaaatct acggcatgcc ggaatatctc ggcgcattac tctcggccag cctgtctcat
    12001 tcggcggaca tgttcagaaa actgtattac gacaacggat cccacgccgg gtgcatcatc
    12061 tacatcggtg ccgcgcaggt aaaccgcgaa agcatggact ccctgaaaga aacgctacag
    12121 ggtgcgcgtg gtggtggtgc gtttaaaaac gtgctcattc atgcgcccaa cgggggcaaa
    12181 gagggggtgc aaattttgcc gttccagcag atcaccgcaa aggatgagtt catgaatgtt
    12241 aaggcggcat cccgtgatga tgtgctggct gcgcaccgcg ttccgccgca actgatgggg
    12301 gcgatgccgg gtgaaaaaag tgcgtttggt gatgtggaga aggccgcgcg ggtttacgca
    12361 attaacgagc tgatgcccgt catggaggcc atgaagcaca tcaatgactg gcttggcgaa
    12421 gaggtgatcc gctttaaccc ttacgcactg ttagacatcc agcccacatc ctgacgcgct
    12481 tcgcttgtct gctgcttcgc cggggcataa aaaatttatg ccccgactct ccagctcctg
    12541 tatcagtcag ataatttcac gacgccttcc agtttatcgc caccatcgac ggtcagactc
    12601 ttacgcaatc ccaccgcgtt gactgcatgt tctcgccgcc tcagtgcgat tttgacggcc
    12661 ttacctttca ccccatcaaa tcagaatccc tcacgtattt ttcacgctca gcgtgagaaa
    12721 tacggccatt ctgtcgtgtc gctgcgacat cgttaaggga acgctattta ccccctgaaa
    12781 cgcgggctgt tcccccgtca cctgcgcgca gaaaaagcgc gtttttttgt gcacgcacgg
    12841 atccttgacg gatccagccg ccacgcgggc tggaagggca aaaattcgtt caaaaaaatt
    12901 gtgcaaatgt gcagcagggc gatgtgcgat tatcgccctg gtttgagggg gatcaggtgt
    12961 tatttttatc tgtcgtcagt gggctaaacc atccacaatt tacatcgcat gactgatgcg
    13021 ttctgtcctc gtgtttctcc tgccacaatg cttttccgcc agcctttatg atggctcttt
    13081 caacatcccg ctcgtatcgc aagggctgaa atacgtcatt aatgaaatat tcgcttatgt
    13141 ccggaagcga tactgcaaaa ttatttgttt gttgcgccgt cctgctatgc agcaaagcgg
    13201 catgctttaa tgcgtcctgt gctttctgga atttaagtgc atagtcagtg gcgatttttt
    13261 ccagttcagc gattcggttg tctttggttt ccagccaatc aagtagcgcc aatacttcag
    13321 gatccccgac atccaacact gttacgcgtg atttttcata atgtttgtca gcaagagttc
    13381 ggccaatttt gaaatctcca tcatcaccat aaccggagca ggcataaaca acctgtgctc
    13441 cagatattcg ctgaattgac atttcctcgc cgcaaatagg gcatttcggt actggcacag
    13501 gtgaataacg ttcacgtaat gcctgatagt tgatttcgct cattaagtcg ttctccttgt
    13561 ttatgttctg aggtggagag cagggcgaac tcgccctgat gttgtgttat tcaggaaata
    13621 acgcccggat atttccggcc atctgactgg ttatctgtgc ggttgatact ggctgtgatg
    13681 cggggcgttc tgtcctggtt tgtgtcactg ataacgcttc atcaccagcc catgcagcca
    13741 gtcgataagc ctctgccgga ttcattttca gaagtgccag cccggccaga aaagccacgc
    13801 gttggccgct tttgcgggct tctggtgtaa ggctgtccag ccaggcgcat gcttctcctt
    13861 cgttcttgac ggcggtgggc ttcagataga aacttatccg tctggttgga gtcgtcattg
    13921 gtttactcct tgtccattgc gtacagccca ttaaccagag caaactgtgg caccccgtcc
    13981 gcgatgaaag tcgcattaac tccgcaggct tcgcggatag cgggtgccac aatctccgcc
    14041 cctccaccga caaccatcac ccgcccgtaa cccgaaaaaa ccgccagcgc gcggatcacg
    14101 cgttgtttca gtgtttcttc cttttcacga attaccgcca tcaggctggc gtaatgcgcg
    14161 tcattgtgga tgtgctggcg cagccaggct tcatcatggc gatgttcgat aatggtattg
    14221 gcgatgtggt gactggtgcg cataccgtta gtggccatca ccgacagtac ggcatcggcc
    14281 atcagagaaa cgcccacgtg tggatcgcaa aacacctggc tgatacctgc cagttgcccc
    14341 tgaacctttg ccacatccag cgtggttccg cccaaatcca caatcagcag ggattcaaac
    14401 ggactcatgt cagccagtgc cttaaagcca gccggaatgg attcaggcat aacctgtacg
    14461 ttacggatag tgaatgcttc gccgttctgg tactccaccg ggcgcatgac gttcgctttt
    14521 ttg
//
LOCUS       DAJTSL010000098        14222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTSL010000098 DAJTSL010000000
VERSION     DAJTSL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.098"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            505..1329
                     /gene="gadX"
                     /locus_tag="OO433_002688"
     CDS             505..1329
                     /gene="gadX"
                     /locus_tag="OO433_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6051108.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1697..2425
                     /gene="gadW"
                     /locus_tag="OO433_002689"
     CDS             1697..2425
                     /gene="gadW"
                     /locus_tag="OO433_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6051109.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSLGTFS"
     gene            complement(2788..5901)
                     /locus_tag="OO433_002690"
     CDS             complement(2788..5901)
                     /locus_tag="OO433_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6051110.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIGAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OO433_002691"
     CDS             complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OO433_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCR6051111.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7143..7421
                     /locus_tag="OO433_002692"
     CDS             7143..7421
                     /locus_tag="OO433_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051112.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OO433_002693"
     CDS             complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OO433_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6051113.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OO433_002694"
     CDS             complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OO433_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6051114.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9575..9907
                     /gene="hdeA"
                     /locus_tag="OO433_002695"
     CDS             9575..9907
                     /gene="hdeA"
                     /locus_tag="OO433_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6051115.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10023..10349
                     /gene="hdeB"
                     /locus_tag="OO433_002696"
     CDS             10023..10349
                     /gene="hdeB"
                     /locus_tag="OO433_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6051116.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10413..11060
                     /locus_tag="OO433_002697"
                     /pseudo
     CDS             10413..11060
                     /locus_tag="OO433_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(11102..11632)
                     /locus_tag="OO433_002698"
     CDS             complement(11102..11632)
                     /locus_tag="OO433_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6051117.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDGMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKITLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OO433_002699"
     CDS             complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OO433_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6051118.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12709..13826)
                     /locus_tag="OO433_002700"
                     /pseudo
     CDS             complement(12709..13826)
                     /locus_tag="OO433_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3946 a         3019 c         3155 g         4102 t
ORIGIN      
        1 attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga agactacaaa
       61 gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca gcagaacagc
      121 tttaaacaca cctgataaca taacgttgta aaaaccgaat gcccagcctt taaaaaaaca
      181 gctgggcatt cggttgctta ttaatcgcaa taatatattg gctgtctatt catcgtgttg
      241 ataagatata tgttatatga atgtttatta aatgtagcga ttgcctttaa tgtcacgaaa
      301 agggattatt tgcttgctat taatttcact gcgttcttaa acgcgttttt gtattataaa
      361 tccagtcatc ctgcccgtag aacatcacac attatcatcc tgttctcccg cttcgtttaa
      421 atttatttat caatcaattt gacttaagag ggcggcgtgc tacattaatt aacagtaata
      481 tgtttatgta atattaagtc aactatgcaa tcattacatg ggaattgtct aattgcgtac
      541 gcaagacata aatatattct caccatggtt aatggtgaat atcgctattt taatggtggt
      601 gacctggttt ttgcggatgc aagccaaatt cgagtagata agtgtgttga aaattttgta
      661 ttagtatcaa gggatacgct ttcattattt ctgccgatgc tcaaggagga ggcattaaat
      721 cttcatgcac ataaaaaaat ttcttcatta ctcgttcatc actgtagcag agatattcct
      781 gtttttcagg aagttgcgca actatcgcag aataagaatc ttcgctatgc agaaatgcta
      841 cgtaaaagag cattaatctt tgcgttgtta tctgtttttc ttgaggatga gcactttata
      901 ccgctgcttt tgaacgtttt gcaaccgaac atgcgaacaa gagtttgtac ggttatcaat
      961 aataatatcg cccatgagtg gacactagcc cgaatcgcca gcgagctgtt gatgagtcca
     1021 agcctgttaa agaaaaaatt gcgcgaagaa gagacatcat attcacagtt gcttactgag
     1081 tgtagaatgc aacgtgcttt gcaacttatt gttatacatg gcttttcaat taagcgagtc
     1141 gcagtgtcct gtggatatca cagcgtgtcg tatttcattt acgtctttcg aaattattat
     1201 gggatgacgc ccacagagta tcaggagcga tcggcgcagg gattgccgaa ccgtgactcg
     1261 gcggcaagta ttgttgcgca aggaaatttt tacggcactg accgttctgc ggaaggaata
     1321 agattataga attttactca gacataaaaa aaacctggca taggggaccg ggaagaggat
     1381 agtctgccgt ctccagacta ataaaccgct ataacactcc ctgttggcac gggaaacttt
     1441 gtgctctcag taagttaaat ataactttta ctggaaataa gatcagccat ttttttataa
     1501 acataagcta tacgctgtgc gaaaatatat tcgtgctgca tttacttatt atcaattaac
     1561 tgttatgcaa aactactttg tggataaatt ttggtcctac caaatctggc agtttttgcg
     1621 ctaagaaaca gtctggcaac atttcattag tatactgaaa ttgaaataat cgcagtatga
     1681 aatataaggg ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga
     1741 tatttatcat gaacagcata aaatatcgct gcataacgag agtatcgtgc tgctggagaa
     1801 aaatttggca gacgattttg cgttttgttc accggatacg cgacgactgg atatcgatga
     1861 gctgacagtt tgccattact tacaaaatat tcgtcagcta ccacgcaatt tagggttaca
     1921 tagcaaagac cgtctattaa ttaaccagtc accccccatg ccgctggtga cggcgatttt
     1981 tgatagcttc aatgaatccg gggtaaattc accgatactg agcaatatgc tctacctttc
     2041 ctgtttatcg atgttttctc ataagaaaga actgatcccc ttacttttca atagcatcag
     2101 cactgtttca ggaaaagttg aacgccttat tagctttgat atcgccaaac gttggtatct
     2161 gcgcgatatc gcggaaagaa tgtataccag cgagagtctc atcaaaaaaa agttgcagga
     2221 tgaaaatacc tgtttcagta aaatattact cgcttccagg atgtcgatgg ccagacgatt
     2281 actcgagtta cgtcaaattc ctctgcatac tattgcagaa aaatgtggct atagcagtac
     2341 gtcgtacttt ataaacacat ttcgacaata ttatggtgta acgccacatc agtttgcgca
     2401 acattcgcta ggtacctttt cctgacatat tttgcatttg aatattggtc aggatctcac
     2461 acctgcttca tgtgaaactc ttccctgatg atttctgccg ggctaccggc tagttctctt
     2521 tcgcagtttg ccaggcggta taggtatttt caggaggaaa agactcggca tgtttgggat
     2581 tattaagctg acaattcata cgattacggg aatgtgggtc agtattgtgc tgttcaaatt
     2641 gatgacgaat ggctggagtg gtttctactt tcagtgctgt gtactaagcc tggtattcct
     2701 gacggttagc tggttgttga gtggtgaatg gttagcagga aagagtaagg ctgaaccttc
     2761 acgttcaacc ttactctcat ttacacgtta cgctttttta aagcgggcaa agagatgttc
     2821 cactacaacg aaaaagaccg gaacgaagta aattgccagc actgttgcgg caaacatccc
     2881 gcccatcacg ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat gactgataac
     2941 cagcggcagc acgccgagaa taaaggccag agaggtcatc aggattgggc gtaaacgcat
     3001 ccgcgccgct tcgatgattg cccctatcgg cgttttccct tctttctgca tcatctcaac
     3061 ggcaaattcg acaatcagga tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc
     3121 aacctggaag tagacgtcat tacttaagcc gcgcagatcg gtggccagta atgcgccaac
     3181 gacgcctaac ggaacaacca acatcaccga gaacggaatt gaccagctct catagagtgc
     3241 ggcgagggcg aggaacacca cgaccagtga aatggcatac agtgcaggag cctggtttga
     3301 ggataacgct tcctgatacg acagtccggt ccatgagtag ccgacgcctg ccggaagttt
     3361 agcgaccagg tctgccataa atttcatagc gtcaccggta cttttcccgg ccgccgcttc
     3421 acctaaaatc tccattgacg ggatgccgtt gtagcgttcc aatcgcggtg aaccataggt
     3481 ccattcggta gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata
     3541 ccattggttg atgttatccg gcaacatacg gaacggcgtg cctgcctgga catacacttt
     3601 tttcacccgc ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat
     3661 tgtctggttg atatcagaca gcgccacacc cattgcctca gctttcgcgg cgttgacgtt
     3721 cactttgaac atcggcgtat cttccaggcc gttcgggcgt accccggtga cctggtcagg
     3781 cgattgcgct gccagtgata acagctcgtt tcgtgcctgg gttagttttt cgtgccccag
     3841 gttaccgttg tccagcagtt ccatatcaaa acctgacgcg gtacccagtt cagccaccgc
     3901 gggtaagttg aacgggaaga cgacggcttt attgatactg cttaacgcaa tcattgcccg
     3961 ctgaatgatc gcggtaaccg agttttcctc accgacacgt tcagaccacg gcttgagact
     4021 gataaacgcc aggccgttgt tttgcccctg gccgctgaag ccaaagccgc caacggtaaa
     4081 caccgactga acattatctt tctcttttgt aagataataa tccgtcacct gttgcagcac
     4141 tttcgtggtg ttaaccatgg tggcaccgga aggtaactgc gcggtggtca taaatacccc
     4201 ctgatcctct tctggtaaga aagaggtcgg tgtacgcagg aacagcaccg ccatcccggc
     4261 gcaaatcagc aggtagacca ccatgtagcg accggtacaa cgcaacagcg agcgggtgct
     4321 atcggtatag tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga acagggcgtt
     4381 aggtttgtga ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg caggggtcag
     4441 gctcattgcc acaaatactg aaagcagcat ggaggagatc agcgtgatgg agaactggcg
     4501 gtagatctcc ccggttgcgc cgctcataaa ggccatcggc ataaacactg cggaaagaac
     4561 aacagcaata ccgaccagcg cacgttggat ctgccccatc gatttatgcg tcgcttcctt
     4621 cggtggtagc ttatcttccg caatgacacg ctcgacgttc tccaccacca cgatggcgtc
     4681 atccaccagt aaccctatcg ccagcaccat cccgaacata gttaacgtgt tgatggtgaa
     4741 accgaccgcc gacaagatcg caaacgtccc gagaataacc accggtacgg caatcgtcgg
     4801 gatgattgtg gcacggaaat tctgcaaaaa cagatacatg accaggaaga cgaggatgat
     4861 agcctcaacc agtgttttaa aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc
     4921 gtaaggataa accgtcttca gacttgcggg gaaataggct gataagcggt tcagctcctc
     4981 tttgactgcc cgcgaggtat ccagggcgtt tgctccggta gccagtttga tggcgatccc
     5041 ggcagcaggt ttgccgttat agcgcgccac ggtggaataa tcttccgctc caagttcgac
     5101 gcgggcgaca tcacgcagca gcacttgcga accatcttgc tgaactttca acaggatttt
     5161 gccaaattct tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg
     5221 gtctgccgcc tgtggcatgc cacccagttg accaccggaa atctggttgt tttgcacctt
     5281 aatctgggaa ataacatcgg aaggtaccag attgtattta ttgagttttt gcgggtccag
     5341 ccagatacgc atggcgtact cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg
     5401 gcttagcggg tctttgatat tagacgctac atagtccgcg atatcgtact ggttgaggct
     5461 gccgttatca gaaataaacg ccgctaccat cagaatatta ctgctcgact tatcaacgct
     5521 aatcccctgc tgctgcaccg cttcaggtaa tgaaggcata gcgagttgca gtttattttg
     5581 cacctgaacc tgtgcgatat caggagatgt cccagtctcg aaggtcagag tgatagaggc
     5641 attacccgcc gcatcactgg ttgaagacat gtacatcagg ccatcaagcc cattcatatt
     5701 ttgctcaatc acctgagtga ccgagtcttc taccgtttgc gcatcggcac ctggataggt
     5761 agcgctgacg gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat
     5821 gatcgccaga ccacctgcaa gcatcataat aatggcaagt acccaggcaa aaaccgggcg
     5881 atcaataaaa tagttagcca tgaaagtccc cttaagcctg caacgttatt gtttcgattc
     5941 ggtgctggcg ttttcctggc tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg
     6001 ttgcaaaccg gaaacgatca cccgatcgcc agcctgcaag ccagaggtga caacccactg
     6061 gtcgccgatg gctttgctgg cttcaatttc gcgtagctgc acaacatcgt ctttatccag
     6121 aatgagcgcc gttgctttac cctgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa
     6181 tacattctgg cggctacctt catccactaa tgccgtgacg tacatgccag gcagcaagtc
     6241 accatttggg ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catccaccgt
     6301 cgggtcggag aatttcagtg tgccggtctg gctgtagcgt ttaccatttt ccagattgag
     6361 ctgtactggc gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat
     6421 gcgcaagaaa tcctgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac
     6481 ggtaaccagc gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt
     6541 cccgctgacg cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg
     6601 ttcaacagcc gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc
     6661 ggtgtcgtaa tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa
     6721 ggtgatgcgg gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt
     6781 tagctcggcc tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt
     6841 atcgccttcg ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc
     6901 aacttcataa ggaacggttc taccgggcaa ttcgctcaac acattgaccg aaccggggga
     6961 gagtgtgacg acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc
     7021 gcaggcggtg agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt
     7081 cattttagtc cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag
     7141 atatgaggga tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca
     7201 ggcacagtga tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag
     7261 ttcctgccag cattcgggca acgaagggtt ctactggtgg atacacatac caggggaata
     7321 aagtcgtttt agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc
     7381 ctcgtcatgc cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata
     7441 cccagggtga cgatgtcgct catacgtctt agtttcaact tgtacataat gttgaatttc
     7501 tgaatcttta atgtttgttg cgaaatattg agcgtcaacg tgatattgct ttcattttta
     7561 tttttcagaa tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc
     7621 ttatggggca agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg
     7681 ctaacaaaat aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca
     7741 ataattctct cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga
     7801 ttgtgatagg tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac
     7861 agggaattga tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga
     7921 aaagaatctt tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac
     7981 atgtcatcct tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc
     8041 tataagaact ttacccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat
     8101 ttatccatca cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg
     8161 taaatagtaa acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa
     8221 ccagtttgtg atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg
     8281 agtctccttg tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga
     8341 aattacctga gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacac
     8401 ggatatttat gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt
     8461 aaccgcccat agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa
     8521 aacattaaac ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc
     8581 catggagtga aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt
     8641 atttcaatga tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg
     8701 atcatagtca catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta
     8761 acgaacaaac tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc
     8821 agtgtggaaa ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg
     8881 acgatatcca gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga
     8941 taccaactca tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca
     9001 atggcaaaaa tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg
     9061 actgcgacga ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac
     9121 ccgacaataa gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata
     9181 tcgccagaga cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca
     9241 ataaactgga ttgctctgcg atgtttttta agcatctcca gatcaaactt caaaattgtt
     9301 gccttatcta tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat
     9361 caatctatgc caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc
     9421 agtatctgat gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt
     9481 ccatcaacat gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgatttgtg
     9541 acggctcttt cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct
     9601 tggtggtctg cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga
     9661 taacaaaaaa ccggtcaact cctggacctg tgaagatttc ctggctgtgg acgaatcctt
     9721 ccagccaact gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc
     9781 ggttttagat gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca
     9841 ggataaacaa gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga
     9901 tatgtaattc cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat
     9961 cttctttaaa agcagctatt cctcctgttc atatataatc tatatattga atgggttaca
    10021 aaatgaatat ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt
    10081 cactggtgaa cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc
    10141 aggaatttat tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg
    10201 aagaaacagt atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa
    10261 ttcctaaagt gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa
    10321 atcaagcatc taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg
    10381 agccatcttg ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta
    10441 tactcgcggc aattgcctgt ggcgctattg gcatggaaag gcaaatgtga ggcaaaggag
    10501 cagggttacg cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga
    10561 aatatggttt tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg
    10621 ctgctcaggt ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc
    10681 aaaatattgt aggtttgacg acggcagcgg atatctggat gaccgccgct ataggtatgg
    10741 ttattggcag tggtatgtac gaattgggta tgtatggttc agtgatgacc ttgctggtgc
    10801 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
    10861 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
    10921 cggatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
    10981 ttcatgccac aacgtcgata gaagatctgt tgcgattatt aaaaggtatg gcaggggtga
    11041 aaggcgtttc aattagctaa tctgtgtggc agcgtagtca gactcaccgt aagcctgaaa
    11101 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
    11161 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgttatt ttcagtttct
    11221 ccagaatatc ctccgttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
    11281 gcgaaagtga cacagccgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
    11341 aatcgatgat agtgatagtc cgcggaacaa aggtcaccgg cactttcccc aggcagcgtt
    11401 taatattaaa gggggagaaa ataatcacat ggacaggttt taatcgttca atttgagtga
    11461 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacaaag gcattctgat
    11521 gcaacattcc gtcgatctct tcttcgttct gtatatgaac gacattacct ttgctcaaaa
    11581 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
    11641 cctggtgcgg acattaattt tataatcagt ttgccaaagg taattaacag gacagggctt
    11701 ataataataa tagttagctt gctgtgtatt aattttatgt aatgcacgat gcagatgctg
    11761 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
    11821 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
    11881 atcccatagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
    11941 catattcact tccaggaagt tatacgggac tttattgata aagccaggtt gttcaccctg
    12001 aatggtaccg aggatggtaa caaagtgatt acgatagttc actgggtcaa ggaagccgct
    12061 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggtttcg catagctatc
    12121 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
    12181 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
    12241 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
    12301 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
    12361 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
    12421 tgaggcgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttttgtttag
    12481 ttcaattcca ttgattttgg ggcgtggcat aagtatccat cgccccataa tataatcctt
    12541 tgttttaaaa ttacataaaa tctattttta ttcaataatt ataaagtgct aataatcaat
    12601 attatggtat tatattctga taatgatttg ataagcgaat tcttttgcca gatggtattt
    12661 ctatttccac cccataattt atatcttttt cattaggaag tggcctcttt aaataaggcg
    12721 tgtgtgacat tttttttggg ttaagcgtct cgaattgaaa tacttcgaac tgatattcag
    12781 acatttctgc ccatgtttga attttattgt atcgagagag tgtcgtagag gctttaactt
    12841 gcttataaca ttgtttttgg aattcttgct gatattttaa tatgtcattg tatgaaagat
    12901 tggttatcct ggcctctaaa aatttaatta ttgtcgaagc aatttccgct tttgctcgta
    12961 aaaagttgtc attaatggtt aataatgttg aaaataaacc ttcgatttgt ttgtcaagat
    13021 attgtccatc tttatataat ttgggggggt agggtgttct ttatgtaaaa aatagtgtca
    13081 cctaaatttg cgtcagtgaa atttatatta agtagatctt cgaaataaaa ccttgacaag
    13141 gttagagcga acccggaaag gttctgtcct gtcatattga aatcaataaa gttggttccg
    13201 gcaaagactt tcatcctgtt tttattttca ctgtgctcaa tatataactt cgtcattttt
    13261 tgatacaggc tttgaatatc ttttatatta gttggtaagt cagctcgttg ttcagcaata
    13321 aaattagtat ctgaaaatat ggtttggaca ctgaatgtta tgggtattcc ctcttctacg
    13381 ctaattttag ttatgattgc cttgtcgggt ttaggattag cgacaacaat gattcgacat
    13441 tcagcacttt tgtttttttc atatctaatg ttattaatct gattaagtat tgattgtgtc
    13501 tgttgcgtaa gtggtgtcga acttatcgtt tcaatgttat taataagact tacaagttga
    13561 accacaactt ttgagtagtc tgcatttgta tatgtggtgt tcgtgtggca tgcattaatt
    13621 tgttggagtt gttgttgcgc taaatcaaat tgttcagggt tgtatccttc tccagagaat
    13681 gtggaattaa tgggttgttg ttcttcccaa ttatcacaag gccacttcgg tgtgtctggc
    13741 gttttaacat tatcggtatt tgttgtgcag ttataaatat tgccgtgata tgtatttatt
    13801 atacctgggg taaagtcaat tgacataaca ttccttattc atcatctgtt ttatgctaag
    13861 gtttttaaat tggataatta gaacaggaat ggattttctt ctacacacaa taaatataat
    13921 gcgttatatc ttcctgatat aattaccttt aaattccctt tcatttccac ccggtaatat
    13981 ttgtgtataa aaaaagcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaag
    14041 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
    14101 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
    14161 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
    14221 ta
//
LOCUS       DAJTSL010000099        14090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTSL010000099 DAJTSL010000000
VERSION     DAJTSL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.099"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..564)
                     /locus_tag="OO433_002701"
     CDS             complement(<1..564)
                     /locus_tag="OO433_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6051119.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQ"
     gene            complement(952..1698)
                     /gene="cutC"
                     /locus_tag="OO433_002702"
     CDS             complement(952..1698)
                     /gene="cutC"
                     /locus_tag="OO433_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6051120.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQWVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLSNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1712..2278)
                     /locus_tag="OO433_002703"
     CDS             complement(1712..2278)
                     /locus_tag="OO433_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6051121.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEYERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            2494..4227
                     /gene="argS"
                     /locus_tag="OO433_002704"
     CDS             2494..4227
                     /gene="argS"
                     /locus_tag="OO433_002704"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6051122.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            4404..4892
                     /locus_tag="OO433_002705"
     CDS             4404..4892
                     /locus_tag="OO433_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR6051123.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDIEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(5012..5404)
                     /gene="flhe"
                     /locus_tag="OO433_002706"
     CDS             complement(5012..5404)
                     /gene="flhe"
                     /locus_tag="OO433_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR6051124.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(5404..7482)
                     /gene="flhA"
                     /locus_tag="OO433_002707"
     CDS             complement(5404..7482)
                     /gene="flhA"
                     /locus_tag="OO433_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCR6051125.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGCEQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(7475..8578)
                     /gene="flhB"
                     /locus_tag="OO433_002708"
                     /pseudo
     CDS             complement(7475..8578)
                     /gene="flhB"
                     /locus_tag="OO433_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            complement(8661..9305)
                     /gene="cheZ"
                     /locus_tag="OO433_002709"
     CDS             complement(8661..9305)
                     /gene="cheZ"
                     /locus_tag="OO433_002709"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6051126.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLAEARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(9316..9705)
                     /gene="cheY"
                     /locus_tag="OO433_002710"
     CDS             complement(9316..9705)
                     /gene="cheY"
                     /locus_tag="OO433_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6051127.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFDKLDM"
     gene            complement(9720..10658)
                     /locus_tag="OO433_002711"
     CDS             complement(9720..10658)
                     /locus_tag="OO433_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HCR6051128.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFG
                     MPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI"
     gene            complement(10661..11473)
                     /gene="cheR"
                     /locus_tag="OO433_002712"
     CDS             complement(10661..11473)
                     /gene="cheR"
                     /locus_tag="OO433_002712"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6051129.1"
                     /translation="MRLPGCSRYSFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRS
                     LGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYR
                     VWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLEKARSGIYRHEELKNL
                     TPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMI
                     YFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            complement(11611..12177)
                     /gene="flhC"
                     /locus_tag="OO433_002713"
     CDS             complement(11611..12177)
                     /gene="flhC"
                     /locus_tag="OO433_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6051130.1"
                     /translation="MSEKSIVQEARDIELITLGARLQMLESETQLSRGRLIKLYKELR
                     GSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLYLEQC
                     PQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPS
                     RAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(12180..12530)
                     /gene="flhD"
                     /locus_tag="OO433_002714"
     CDS             complement(12180..12530)
                     /gene="flhD"
                     /locus_tag="OO433_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR6051131.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVNDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13308..13736
                     /gene="uspC"
                     /locus_tag="OO433_002715"
     CDS             13308..13736
                     /gene="uspC"
                     /locus_tag="OO433_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR6051132.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13743..>14090)
                     /locus_tag="OO433_002716"
     CDS             complement(13743..>14090)
                     /locus_tag="OO433_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCR6051133.1"
                     /translation="LRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIVNP
                     YDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRSAES
                     QQRDKVATFPKLA"
BASE COUNT         3618 a         3728 c         3290 g         3454 t
ORIGIN      
        1 ctgactttcc agctcatgcg ccgcgttgtc atccattttt atttctggcg gaaaatgggc
       61 aatgccttta tgacaatcga tacaggtttc gccgcccttt tgtgctttgt tatgcatttt
      121 ctgcgcagat tcactttgcg aggcaatatc catggcatca aaagaatggc aactacggca
      181 cgttgcagag tcagttgctt ttaattcttt ccatactgtt tcggccattt cctggcgatg
      241 agtttcgaac ttatcgtcac tgtctatttt gccgctaaca aattcatgat aaatatcttt
      301 agatgctttt aatttagcaa ataaataatc catccctgac tttggaatat ggcaatctgc
      361 acattccgca cgtatccctt tctggttcga aaagtggaca gttccctgat attcctcaaa
      421 aggtttactc atcgagtggc aggaaaggca aaatgctgta tccgacgttt tatgtaaggc
      481 tttttgcgcc agcaataacc cgccgccacc aaccacaacc gctatcagca aaaacaataa
      541 cccaatgcgt tttttccctc gcatagattc ctctaaataa attccagaac accgaaatca
      601 ttggataatt aaatacacaa cgaattaacc gcttgcataa ctcggcacaa cactgcctga
      661 aataatcgat aaagaatatg atttattaca atgtaatcat taattgctaa ggaataaccc
      721 tgttgttatt tagaatatat atttgtcagt tttagcaatt taaaccccat aattaatgtg
      781 aatatataaa atatttttag cacaacgcta tgttcactgt cacccgctca acagatcacg
      841 gttatcgtac gttttttata ctgctcaggg ataaaaaaag gcccctgtcg aaattgcagg
      901 ggcctggtac gagcaagcat catattgggc gacatgatgc aacggtaaaa atcatttggc
      961 ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc gccccgtcta cgacataacg
     1021 cgaatactcg tccgcgtgtg catctgatga catggacaat ccttgattac gataacgcat
     1081 cggtgaagct tgccacgctc ccgcggagct atggacttcc agcactccgg catcgaggaa
     1141 gtggtgcagg ttttctgcac ggactcctgc tccggccata atgattggag catcacgatg
     1201 ggcaataagt tccataattt ttgataaacc ttgcagtgca tctgattttt gccctgatgt
     1261 cagcacccgg gcgatgccca attccgcaag attactgagt gtatttaaag ggttagcgca
     1321 catatcgaag gcgcgatgaa aagtcactgc cagcggaccg gcagcagcca ttattttttc
     1381 cattcgtggc atatcgacat tcccgtcaac atcgagcacg cccgtcacca gtccaggaaa
     1441 acctaattcg cggactgtgc gcacatcctc aagaatggcg gcaaactcac cgtcgctgta
     1501 gcaaaaatca ccaccgcgtg ggcggataat cggatgcaca gggatcgtca cccactggcg
     1561 cacggatttc agtacaccca acgacggcgt taagcccccc tcttttgggg ctgcgcataa
     1621 ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca cattccatgc tgtaacagca
     1681 aatttccagt aatgccattt ttactcctta attacgccga ctgctcgctg gcaacgatct
     1741 cttcaatgga ccacggatga aacttaatag ttgttttgcc atcagtgacc gccagcgtcg
     1801 gattcggtaa tcgctcatac tcaccttttg gcgaactggt tttcactgag atccccggta
     1861 ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc attcagcgtt tctggatcac
     1921 ccggcagaac gatttcaata tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc
     1981 acggtagctc cacaatggaa aactgccagt gcgcaacctg taccggttca tgcaatttaa
     2041 acagacaaat cggtctgcca ttgatcatat tttctgacaa aagctcgcca cactgttcaa
     2101 aaccgcgacg ccagcgctca gcggtgacgt tttgatggca acgcaaagaa atgtgatcgg
     2161 cagtcagcgg agtgatatcc aaccccagac ggcgggagag ttcatctaat gcgtgggtaa
     2221 atcgcggtaa atccgatgca atatcctgca gctcgtcgat agattgccag ttcgccataa
     2281 tcactcttcg tctttcagta aaagcgttaa tttaccctgt tgccctgcgc caaccaaccg
     2341 ctgatttcac gccgcttctg atgcaatagt gaaaacggca atacgccacg cgcacgttgc
     2401 tgacgaaaac agccatttgc agtatactcc cgccctaatt tctttaactg gtgcgggcaa
     2461 tttttgctcg cttcatcaat gtaaggtatt ccggtgaata ttcaggctct tctctcagaa
     2521 aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg attgcgaacc gcaggttcgt
     2581 cagtcagcaa aagttcagtt cggcgactat caggctaacg gcatgatggc agttgctaaa
     2641 aaactgggta tggcaccgcg acaattagca gagcaggtgc tgactcatct ggatcttaac
     2701 ggtatcgcca gcaaagttga gatcgccggt ccaggcttta tcaacatttt ccttgatccg
     2761 gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg atcgtctcgg tgtttctacg
     2821 ccagaaaaac agaccattgt ggttgactac tctgcgccaa acgtggcgaa agagatgcat
     2881 gtcggtcacc tgcgctctac cattattggt gacgcagcag tgcgtactct ggagttcctc
     2941 ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg gcactcagtt cggtatgctg
     3001 attgcatggc tggaaaagca gcagcaggaa aacgccggtg aaatggagct ggctgacctt
     3061 gaaggtttct accgcgatgc gaaaaagcat tacgacgaag atgaagagtt tgccgagcgt
     3121 gcacgtaact acgtggtaaa actgcaaagc ggtgacgaat atttccgcga gatgtggcgc
     3181 aaactggtcg acatcaccat gacgcagaac cagatcacct acgatcgtct caacgtgacg
     3241 ctgacccgtg atgacgtgat gggcgaaagc ctctacaacc caatgctgcc aggaattgtg
     3301 gcggatctca aagccaaagg tctggcagta gaaagcgaag gggcgaccgt cgtattcctt
     3361 gatgagttta aaaacaagga aggcgaaccg atgggcgtga tcattcagaa gaaagatggc
     3421 ggctatctct acaccaccac tgatatcgcc tgtgcgaaat atcgttatga aacactgcat
     3481 gccgatcgcg tgctgtatta catcgactcc cgtcagcatc aacacctgat gcaggcatgg
     3541 gcgatcgtcc gtaaagcagg ctatgtaccg gaatccgtac cgctggaaca ccacatgttc
     3601 ggcatgatgc tgggtaaaga cggcaaaccg ttcaaaaccc gcgcgggtgg tacagtgaaa
     3661 ctggccgatc tgctggatga agccttggaa cgtgcacgcc gtctggtggc agaaaagaac
     3721 ccggatatgc cagctgacga gctggaaaaa ctggctaacg cggttggtat tggtgcggtg
     3781 aaatatgcgg atctctccaa aaaccgcacc acggactaca tcttcgactg ggacaacatg
     3841 ctggcgtttg agggtaatac cgcgccatac atgcagtatg cttacacgcg tgtattgtcc
     3901 gtgttccgta aggcagaaat taacgaagag caactggctg cagctccggt aatcatccgt
     3961 gaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt ttgaagaaac cctcaccgtg
     4021 gttgcccgtg aaggcacgcc gcatgtgatg tgtgcttacc tgtacgatct ggctggtctg
     4081 ttctctggct tctacgagca ctgcccgatc ctcagcgcag aaaacgaaga agtgcgtaac
     4141 agccgtctga aactggcaca actgacagcg aagacgctaa agctgggtct ggatacgctg
     4201 ggtattgaga cagtagagcg tatgtaatcg atttttcgtg agagtaaagc ctgatcaggg
     4261 ttacctgatc aggctttttt attgccatct aaatgtattc tgaaaatgga catgccattg
     4321 ttttctgact gttggataag aggccagaag cgtaatatcc ggccccaggg aaacgataac
     4381 ggttgaattt aaggaatacc gcagtgttta aatttcttgt attaacatta ggcattatct
     4441 cttgccaggc ttacgcagaa gatacggtta tagtaaacga ccatgacatt tcagccatca
     4501 aagattgttg gcaaaaaaat tcagatgatg atactgacat taacgtgatc aaatcatgcc
     4561 tgcgacaaga atacaatctc gtcgatgcgc aattaaataa agcctatggt gaagcttatc
     4621 gttatataga acaagtgcca cgcacaggtg taaaaaaacc tgatatcgaa caacttaact
     4681 tgcttaaaaa atcacagcga gcctggctgg attttaggga caaagaatgt gaattaatcc
     4741 tttcaaatga ggacgttcag gatttaagtg acccttattc tgaatcagaa tggctctcat
     4801 gtatgatcat acagaccaat acgcgaactc gccagttgca gctataccgt aactctgaag
     4861 atttttatcc aagccctttg acaagaggat aattcacatc tttttggcat gttttgttgc
     4921 aagctattcc tgataaataa ttgcaacaag acatcgagcc tttttcactg agttattaaa
     4981 catactcgcg agcgcgtaat ttttttgtcc ttcagcgata attcacaatc acttcattac
     5041 gttgtacctt tagcggtgga attaaccgcc caccgcctgg cacttcccag ataaatcgca
     5101 acggttctgc tgccgctata ccggaaaagg ccacggtggt tccgctctgc ccctctaatt
     5161 cgacacaacg agattgcgaa cacaagcgaa cccgcagtcc tgaaggtgtc gggccgataa
     5221 gctgataacg ccacgctacc agcgtcatca atcctgaagc aggttgtcgc gtagaaagag
     5281 gcgcagacga catcgactca ccgcgatgat ttagcgtaat tcctacacta cttgcctgcc
     5341 acatcccctc cccggcggct tgcaccagca gcggaaacaa taatattgct aataaggttc
     5401 tcattatttg ccgccaattg tcgccgtcat gcggatatgt cggttatcag acagttccag
     5461 attcgacagg accactaact gcggcaagct gcggcgcagg aagcgagaca ataatggtcg
     5521 cagcgcgtgg ttcaccaaca atactggcgg cgcacccagc atctcctgac gggatagcgc
     5581 ttcctgagtt tgcgccagta aacgatccgc cagccctggc tccagtcctc ccccgccctg
     5641 cagcgcctgt agtaacaaac gttccagcgg tgtatcgagg ccaataacat ggacttcatc
     5701 tttgccagga aaccactgct gggtaatcgc ccgtcccaac gccacgcgca cgacggcggt
     5761 taattcatgt ggatcgcttt ggatgggcgc atgttccgcc agcgtttcga gaatggtgcg
     5821 catatcgcga atcggtactt tttcatcgag gagattttgc agcactttat gcagtgtggt
     5881 gagcgtgacg acgccaggaa cgagatcttc cgtcagcttt ggcatctcct gggcgacgcg
     5941 atccaacagc tgttgcgcct cctgacgacc aaacagctct gcggcatgct ggctaatgag
     6001 gtggttaaga tgcgttgcta ccaccgtgct ggcctcaacc actgtgtacc cctgaatctg
     6061 cgcctgttct tttagcgcac tttcaatcca gatagcattc aggccaaatg ccggatcgac
     6121 ggtcgcctca ccaggtaacg tcccggcagc ggttccaggg ttaatcgcca accagcgccc
     6181 cggataagca tcaccactgc caatctccac gcctttcatc aaaatgcgat agcgggcagg
     6241 ttgcagatcc atattgtcgc gaatgtgcac cactggcggc agaaatccca tctcctgggc
     6301 aaatttcttg cggatactgc gtatacggcc caacaactca ccatcctgct ggaaatcgac
     6361 catcgggatc agtcgataac ccacttccat tcccagagaa tcttccagtt gtacatcgtt
     6421 ccacgtcgct tcgacaacgg tattattctc tgccattttt accggtttgg gttcggcagg
     6481 cgctttttgt tcgcatccgc gtatccacca ggccagcccc agcaatccgg cagtgaacag
     6541 caaaaatacc aggttcggca ttccaggcac caagccgagt aaaccgagca cggcggcgct
     6601 taacaacata acgcttgggt tactgaaaag ctgattcacc atctgctcgc caacatcctg
     6661 atcggtgctg acacgcgtaa cgatgacccc cgcggcggta gaaatcacca gcgccggaat
     6721 ttgtgccacc agaccgtcgc caatggtcaa tagcgtataa ctttccgccg cgtgtcccat
     6781 gctcatgcca tgttgcagca cgccgaccag caacccgccg acaacgttaa tgaccatgat
     6841 gaggatcccg gcgatggcat cgccgcgaac aaacttactt gccccgtcca ttgagccgta
     6901 aaaatcggct tcctgagtca cttcggagcg gcgttttttc gcctcatctt caccaatcaa
     6961 tccagcgtta aggtcggcgt caatcgccat ctgcttaccc ggcataccat cgagaacaaa
     7021 gcgcgcaccc acttctgcga tacgcccggc acctttggta atgaccataa agttgatgat
     7081 cacgagaatg acaaacacca cgatacctat agcgaaattg ccaccaacga ggaagtgacc
     7141 gaacgcttcg accaccttcc ctgccgccgc cgcgccggta tgcccttcca tcaaaatgat
     7201 acgggttgaa gccacgttaa gtgccagacg caacaacgta gtaaacaaca gaatggtcgg
     7261 aaacgcagca aactcaagcg tgcgctgggt aaacatcgcc accagcaaca ccatgatcga
     7321 caaggcaata ttgaaggtaa acaacaggtc gagtatgaat gcgggcagtg gcagcaccat
     7381 catcgacaag atcaacagga tcaaaatcgg tccggcaagg atctgccatt gtgtcgattt
     7441 caggtttgcg ggcaggcgca gcatcgcggc cagattactc atgggtcggt ttctcgttaa
     7501 taaaatccag ggcttccggc accggaagat gagtaggttg tacagggcgc tgtccaccag
     7561 ccagacgcca gcgtttcagt tgccagatcc aggccagcac ttccgccacc gcggcgtaca
     7621 gttgacccgg gatttgttga ccaatctccg catgtcgata cagcgctcgc gccagcggcg
     7681 gcgcttcaag cgtcgggacg ttattttcag cgccaatttc acgaatgcgc agcgcgacca
     7741 gccctgcacc tttagcgacc actttcggtg cgctcatttt gttttcgtca tactgcaacg
     7801 ctaccgaata gtgggtcggg ttattgacaa tgacatccgc tttcggcaca tcggccatca
     7861 tccgacgccg tgcggcagct cgctgcatct gacggatccg tcctttaaca tgggggtcgc
     7921 cttcgctttg tttgaactca tcacgaatat cctgccgcga cattcgcagc tttttcaggt
     7981 ggctgaagat ttggaaaaag acgtcaaatc ccaccattgg aatgacacca agcaccacca
     8041 gcagtgcgca tagccctacc aaatccatcg cattacccat ggcggtaatc ggagactcgg
     8101 ccagtaagcg catcatctgc ggccagtgat gccagagaaa aaaccccgtc acgctgccaa
     8161 ccaagatggt tttcaaaatt gctttaagca actccgcgcc agtctgtgcc gagaacatcc
     8221 gtttaatgcc cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat tttccgctaa
     8281 ataccagccc ccccagcatg accggagaaa tgagcgctac cagcaccacg ccgctaatca
     8341 gcggtagcag cgccagcatg gcttctctga tcagcagaat aatctgcccg aggatcagat
     8401 tcgggtcttt gataatactg tgatcaaaat gcagcccagc ggagagcatg cccgacaatc
     8461 gacgggccag cgacacaccg ccgaaccaga taacacacac gcccaccagc aaaatcagca
     8521 gtgaggtcag ttcacgggaa cgcgggattt agggcttttg ctgccacaca tcaagcatag
     8581 tgtgcgtttg tcggatgcgg cgtcatcgcc ttatcagacc gcctgatatg acgtggtcac
     8641 gccacatcag gcaatacaaa tcaaaatcca agactatcca acaaatcgtc cacctgatcc
     8701 tgactggcta ccacaccggc tttgctggta tcgacctgag gtccattaag caaactctgg
     8761 ttttcacgtt ttggacgcga ctcctgttcc gggatgtttt ccaacagcac catcagcaac
     8821 tggcgttcga tctcctgaat gacatccatc atccgcttaa taacctgccc ggtgagatcc
     8881 tgaaaatcct gcgccatcat gatttccagc agttgcgcgt tagtaaagct ggtatgcgcg
     8941 ggtacatctg ccagaaattg tcgtgtatct gttaccagtt cacgggcctc ggcaaggtca
     9001 atcggatcgg caaaccagtc atcccaacgt tgggttaacg cttttgctga tttctccatt
     9061 tgatcctgat gcggttgtga cgcctcaaca ctgttcagcg cccgctccgc agcctgggcg
     9121 gtcatctgca caacatagta caaacgatcg cgcgcatcgg ggatggcttc cgccgcttcg
     9181 gcaatggcct gatccagccc cagttcccgc aaactgtcgc gcagcatacg cgtcaggctg
     9241 ccgatgcgcg caatgatatc gccagctgaa tgctcgtcag caggtttgat tgatggttgc
     9301 atcatagtcg catcctcaca tgtccagttt atcaaagatt ttgttgagtt tttcctccag
     9361 cgtcgcggcg gtaaatggct tcaccacata gccactggcc cccgcttgcg ccgcagcaat
     9421 gatgttctct ttcttcgctt ctgcagtcac cattaacact ggcaatgccg acatcgcgcc
     9481 atccgcacga attgttttca gcaattccag gccatccata ttgggcatgt tccagtcgga
     9541 gataacaaat ccataaccgc ctgcctgcaa cttattgaga gcgtcgacgc catcttccgc
     9601 ttcctcaaca ttattgaatc ccagctcttt cagcaggtta cgcactatgc gtcgcatggt
     9661 ggaaaagtca tccacaacca aaaatttaag ttctttatcc gccatttcac actcctgatt
     9721 taaatacgta tcgcctgtcc ggcactaatt tttgccaaca tttgctggct tacctggcta
     9781 agatcgacca cttcgcagac accacccata ttgatggcct cgcgcggcat gccgaacacc
     9841 acgcaacttg cttcgttttg cgcaagggtc catgcccccg cctgacgcat cgccaacatt
     9901 cccaccgggc cgtcgtgaat tttgatttga taatttgcgc cactacgcgc cagttccata
     9961 tgccgatcgc ccggcgcaat ataggcatgc cccggcaaga cacgttctcc gtcttcggct
    10021 tctttaaccc cgatctggca aagcttatta agtctgtcgg caaaagagcg ggtgaaaccg
    10081 ggcggcatat gttgggtaat taacaatgcc gggctggaaa gcggcaacgg ttgcagtacg
    10141 tgacgaattg cctcagttcc acccgttgaa gcaccaatcg caatcagttt ttcagaactc
    10201 aacaacggac ccgccttcag cgttgtcggt gctgacaatg gcttatgtgc tgcaaggctc
    10261 gcctttgctg ccgtacgcac cttttcagca atcatttcgc tatacgccag cattccttcg
    10321 cgaataccca gttgcggttt ggtgacaaaa tctattgccc ccagctccag cgcgcgcagc
    10381 gtgacttctg atcctttgcc ggtcaaggaa gaaaccatca caacgggcat tggacgcaaa
    10441 cgcattaatt tttcgaggaa atccagtccg tccatccgcg gcatttcaac atccagcgtc
    10501 agcacatcgg gattgaattt cttaatcaag tcacgcgcga ccagcggatc aggcgcggtc
    10561 gccaccattt ccatgtcgct atggctgttg atgatttctg tcatgatctg gcgcatcagt
    10621 gccgaatcat cgacagataa caccctgatt ttgctcatcg ttaatcctta cttagcgcat
    10681 acaccgtctg accacgcagc gtgaagcggc gctcaaggtg gctaaagttt tcagagtgac
    10741 ccgcaaacag caatccgtcg ggtttaagga gcggaacaaa gcggcgcaaa atctcctgct
    10801 gggtattttg atcgaagtag atcatgacgt tacgacagaa gatcgcatca aacggccccg
    10861 gcacggtgta ctgtttcgcc agtagattca gcggggcaaa atcaacatag ttcgccagct
    10921 cctgacgcac gcgtaccagc ccttcatgcg gtcccgtccc tcgcatgaaa taccgttgca
    10981 gttgctgcgg cgtcaggttt ttcaactctt catggcgata aataccgctt ctggcttttt
    11041 ccagcacttc ggtgtcgata tcactggcaa acactttcca gcgtccgggc gcggtgccca
    11101 atgtgtcagc cagcgtcatc gcaatgctgt acggctcttc gccggttgaa gccgccgcgc
    11161 tccatacgcg atactcgcca gaacggcgac gtgcgtgatc cgcgagcaga gggaaatgat
    11221 gtgcctcacg gaaaaatgcc gtcagattcg tggtcagcga attgataaac gcctgccact
    11281 caccgctgtg ctgattagat tccagcaagt tcagataatg accgaaatcc gtcagtccca
    11341 gcgaacgcaa acgacgaacc agtcggttgt aaaccatgtc gcgtttatga tcggccagga
    11401 cgatcccggc tcgttgatag atcaattgac ttatccgccg aaaactgtac cgagaacaac
    11461 caggtaacct aataagataa gcacgacatc atccttccac tgttgaccat gacaggatgt
    11521 tcagtcgtca ggcgttaacg cgcgattggg gcaaaaaaaa gcagcggtac gtcgttaccg
    11581 ctgctggcat gttgcgccac accgtatcag ttaaacagcc tgtactctct gttcatccag
    11641 cagttgtggg ataatatcgg caggattctg ggaaagttta cgtcttttta ctgcccggga
    11701 tggcggttga cataagctgc aggcaaagct gccaacaggc tggtgagcgt gggtaataaa
    11761 attgccgccg cagcagttgc agctggaaag ttgcagtaat ccactttcaa caaaccgcac
    11821 caatgtccag gcacgggtta atgccagcag tggtccttct tctgcttgtg ggcactgttc
    11881 aaggtataaa cggtaggctt tgatcaccgc atcgacgcca ttacacaaac ctgttttcag
    11941 taaaaactgc catgcattac agaacatcga agcatgaacg ttttgttccc aggtcataaa
    12001 ccagtcggtt gagaatggca gcatgccttt cggcggtggg cttccgcgca gttctttata
    12061 aagttttatc aggcgtccgc gacttaactg tgtttcgctt tccagcatct gcaaacgagc
    12121 gcccagggtg atcaattcaa tatcccgcgc ttcctgaaca atgctttttt cactcatgat
    12181 caggcccttt tcttgcgcag cgcttcttca ggctgattaa catcattcag caagcgtgtt
    12241 gagagcatga tgccggtatg aatttgctgg agatcgttaa cgcgggaatc ttgcgtcaac
    12301 tgagtaatcg tctggtggct gtcaaaacgg aagtgacaaa ccagttgatt ggtttctgcc
    12361 agcttaacca tttgcggaag agtcagtgcc gctaacgttg tcgccatttc ttcatttatg
    12421 ccgagacgaa acatagcgga cgctttgtcc tgaacaatca aacgctgtgc aagtagtaaa
    12481 tatgacaagt tgatgtcata aatgtgtttc agcaactcgg aggtatgcat tattcccacc
    12541 cagaataacc aactttattt ttatgcggtt tcaccgcacc ccgtgatgtc gccgggaagc
    12601 cccggtaaaa aataattagc attagaatag tgcgataagc tgcaataagc agaaccacct
    12661 ttttggttta atatgtcctt acaaatagaa atgggtcttt acacttatct aagatttttc
    12721 ctaaatcgac gcaactgtac tcgtcactac acgcacatac aacggagggg gctgcgattt
    12781 tcaataatgt gtgatgcaga tcacacaaaa cactcaatta cttaacataa atgtataagc
    12841 catacttttg ttttgggtgt atttcaatct gttaaaaagt ttttcgctac gctagcaagc
    12901 aaaaatgaaa ctggaataat cgaaatggga tgttgcgcac agttaaaata actcaccgta
    12961 aataatcatc tgctataaat aatcactttc atgcaatacc agataagcta tttttaaaca
    13021 gacacttacc gcacaacaaa ctaattaaat acaaagaaag gcaacaatca acttaatgtt
    13081 aatgaaatgt tttacatata ttaaacactt gtttaatgcg gagaatcagt acactatttt
    13141 cttataatta catttgaaat tatatgatca gcgggagtga ttgattaatt gtctttacta
    13201 ataatcagac taatatttac ctgtttgacc gagttgggat tgcgtcgttt ctccattagg
    13261 agtaaagctt taatgtcacc tgaagttcac agaataagga acaggttatg agctatagca
    13321 atattcttgt cgctgttgcg gttacaccgg aaagtcagca actgctggca aaagcggtat
    13381 ctatcgccag gccagtaaag ggacacatca gtttaattac tctcgcttcc gacccggaaa
    13441 tgtacaatca attagccgcg ccgatgcttg aagatttacg cagcgtgatg caagaagaaa
    13501 cgcaaagctt tcttgataag ttaattcagg atgcggggta tcccgttgac aagacgttta
    13561 ttgcctacgg tgagttaagc gaacatattc tggaagtatg tcacaaacat catttcgatt
    13621 tggtgatttg cggtaatcac aatcacagtt tcttttcgcg agcatcctgc tcggcgaaaa
    13681 gagtgattgc ctcaagtgag gtcgatgtgc tgttagttcc acttacgggt gattaaccgc
    13741 tcctacgcaa gctttggaaa ggtagcaact ttatcgcgct gctggctttc cgcgcttcgc
    13801 ggaactatct gctttaggtc gctaatgaag cactcctgcc agtggttaat atcgtttttc
    13861 acgataacgt ccagcatttc tgcatgacgg gaaatacgtt ccgccagcga catagtcaat
    13921 gcacgatcca gcgcagctgc aacttcgtca cgatcgtagg ggttaacaat taacgccgac
    13981 gttaactcgt ttgccgctcc cgcaaattgc gaaagaacaa gaacgcccgg gttggctggg
    14041 tcctgagcag caacatactc ttttgctacc aggttcatcc cgtcacgcag
//
LOCUS       DAJTSL010000100        13921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTSL010000100 DAJTSL010000000
VERSION     DAJTSL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.100"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..284)
                     /gene="fimH"
                     /locus_tag="OO433_002717"
     CDS             complement(<1..284)
                     /gene="fimH"
                     /locus_tag="OO433_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR6051134.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGT"
     gene            complement(304..807)
                     /gene="fimG"
                     /locus_tag="OO433_002718"
     CDS             complement(304..807)
                     /gene="fimG"
                     /locus_tag="OO433_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6051135.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGETKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(820..1350)
                     /gene="fimF"
                     /locus_tag="OO433_002719"
     CDS             complement(820..1350)
                     /gene="fimF"
                     /locus_tag="OO433_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR6051136.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(1360..3996)
                     /gene="fimD"
                     /locus_tag="OO433_002720"
                     /pseudo
     CDS             complement(1360..3996)
                     /gene="fimD"
                     /locus_tag="OO433_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
     gene            complement(4063..4788)
                     /gene="fimC"
                     /locus_tag="OO433_002721"
     CDS             complement(4063..4788)
                     /gene="fimC"
                     /locus_tag="OO433_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6051137.1"
                     /translation="MSNKNANVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWMENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQFAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(4825..5364)
                     /locus_tag="OO433_002722"
     CDS             complement(4825..5364)
                     /locus_tag="OO433_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6051138.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RWVTGGIANAQAWFSLTYQ"
     gene            complement(5429..5977)
                     /gene="fimA"
                     /locus_tag="OO433_002723"
     CDS             complement(5429..5977)
                     /gene="fimA"
                     /locus_tag="OO433_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6051139.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQIVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
     gene            complement(6458..7054)
                     /gene="fimE"
                     /locus_tag="OO433_002724"
     CDS             complement(6458..7054)
                     /gene="fimE"
                     /locus_tag="OO433_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6051140.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(7532..8134)
                     /gene="fimB"
                     /locus_tag="OO433_002725"
     CDS             complement(7532..8134)
                     /gene="fimB"
                     /locus_tag="OO433_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HCR6051141.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTPPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            9590..10306
                     /gene="nanC"
                     /locus_tag="OO433_002726"
     CDS             9590..10306
                     /gene="nanC"
                     /locus_tag="OO433_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6051142.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSSDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            10326..11432
                     /gene="nanM"
                     /locus_tag="OO433_002727"
     CDS             10326..11432
                     /gene="nanM"
                     /locus_tag="OO433_002727"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6051143.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNREGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQHGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            11497..12477
                     /locus_tag="OO433_002728"
     CDS             11497..12477
                     /locus_tag="OO433_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6051144.1"
                     /translation="MDAIISPDYYYVLTIAGQSNAMAYGEGLPLPAREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLSFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLIMGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWREK"
     gene            complement(12485..12607)
                     /locus_tag="OO433_002729"
     CDS             complement(12485..12607)
                     /locus_tag="OO433_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6051145.1"
                     /translation="MISFVFLLLVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            12689..13561
                     /locus_tag="OO433_002730"
     CDS             12689..13561
                     /locus_tag="OO433_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6051146.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGSVERLFWDFDGKIIALPQVRKNYPY
                     EGFVEWHRNEVFRG"
     gene            13606..13785
                     /locus_tag="OO433_002731"
     CDS             13606..13785
                     /locus_tag="OO433_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051147.1"
                     /translation="MQDLAASLQLAFDSGAKRVLLPISWAMDIPTVPAELFTKFQVSF
                     YSEPVDAVYKALDVN"
BASE COUNT         3912 a         3194 c         3026 g         3789 t
ORIGIN      
        1 gtcccggaaa aattagataa cacgccgcca taagccgagc ctcgttgcag tgtgacatag
       61 tctgtaatgg tttccggata atcgttatgg caaaagattt gcgtcgaaag atctacgacc
      121 aggttttgcc ccacattcac gacaggcgca aggtttacat aaacattagc gctgccaccg
      181 ccaataggga tagcagtacc attggcggtt ttacaggcga atgaccaggc atttaccgac
      241 cagcccatca gcagtacagc aaacagggta ataactcgtt tcattacaat tatctcttcg
      301 ggttcagctg taggtatagg tgatgctaat cactgcctga atggttccct gagtggctcc
      361 gccatttacc gtcaatgctc tgacctgtaa cgggaagtgc gctgattgtg aggaatcatc
      421 cacctgaact gttttggttt cgccagtatt caatgtgttg ccactgtcat cctgtagctc
      481 taactggatg ttttgcgcgg tcccctggtt tttataatat ccggtactgt cggctgcccc
      541 gctgaagctg gcagtgaccc tcgacgttcc caccggacaa ttagtcaact caagcgcaac
      601 atcatgccag gccgatgccg ccccggcaga catcagactg aaagaataaa gatcgccgag
      661 atcaaccgtg gcattggtgg tggaaactgt gcacggtttg gcgacgacct taccgttcac
      721 cgtgatggtg acatcggctg cctgtattgt cgcacttgcg agcgccaata ttgccgccaa
      781 taaataccca cgtttgcgcc atttcatgag catctccagt tactgatatt caagagtgaa
      841 ggtagccgtg gcattgatat gccccgcagt gacaggcacc tgtgtcgcca ttagccgggc
      901 gtaaaaattc aacgtatttg gtttacccgg cgtcagggtc gtccacgaaa tcgcggacga
      961 tggagcatta aggggtatct gattttgctg ctcattcaga agctgtattc ccagtcccga
     1021 agccgctgac accgtatttt caagtgcaag caggttggca ttgtggctat ctgcaacgcc
     1081 ggtaaaccca acctttacgg cagaaacggc gttaccacag ggtgacagca aaatacgaaa
     1141 tggaacgaca ggagtcgttg cgccaatgtt gttaaattgc ttcgccgcgt tttccatcag
     1201 atcaacagta aaattggttg attcagcggc cacactacag ccgttatctc tgacatagcc
     1261 gcggatagta atcgtgctat ccgcagccaa aacgcgactt accgccagcc acaaaaaagc
     1321 gcacagaaga taaaaaggtt tgtttctcat cacgccccct taacgacatt cagctgatag
     1381 ctgggttaat aactgctgct gactctctgg tggcagttga taattggcga tacagtgagc
     1441 attttcctct tctccccatt tcacctgaac ttttcccgct aaaggcattc cgctgaggta
     1501 aacctgacca ttatccgcaa caatgccgct actctggcta ctctctgatg tcaccatcgc
     1561 cccaaacggc agcggcttat tattgtgggt tagcgtcatg agcagtttta tcccaacgcg
     1621 cgctttaaac tctgctcgca cgatcgcccc acgagtggga acaacgttag cgaccgcgtt
     1681 atctaaatcg acgttatcag ccagggtatt ggtatccagc gccactctgt tttcccgata
     1741 ttcggtggca taaggcagca cggcataacc gcgccagtcg gtacgcaccc ccgtctggtt
     1801 ttcgactttt gcatcttttg cgccaggcgc tttaacaagt accaccgtat cgtttaacgg
     1861 ctgccccagc gttacgccat tggcatgagc cagtacccca ccgctgactc cgtaatagag
     1921 ctgcttaata tcatcgctat ggctgtaacc gatattggca ttgccgtaac caccgcgata
     1981 attcagcgtg gcgtagcctg tacttccgct attaccatcg cctcccccgg catagccggt
     2041 ttgcacgcta tagctgaggt tgttgtcttc cagcaacgta ccgtatacac cagccagatt
     2101 ggtcatccga ccgttgagat cgtgtgacac gctgtagctg gcactggcat gtcgccactg
     2161 agatttactg tcagaacgca gccagtggct gaaaggaata ttgacgttaa gcgctaacat
     2221 ctgatcccgc cctttttgcc aggcgttttt cgtcaggcta tagctgagcg tccagttgat
     2281 atcttcgaac gcagtattta atccagccta gaattgctca tcgacattac tcgttcccca
     2341 ataagtttga tggctaccac tcaaatacag tgttgatgtg cgcccgagtt gctgagtaac
     2401 ggtgagctgt aatttcccgc gtttgttata agcgaggttg taatagtcgg tgaatttcgg
     2461 cttaacctga ataactccat cctgtgtttc gatgttgtag ccattcattc gactgtatgt
     2521 tgtatcagcg aaattaaaat atccgctggt cgaataacgg taacccacta actgaatatt
     2581 cgtgcctgac tcattgagcg atttgttata gagaaaacgc accgattgtc cgtcatgctg
     2641 actgtcatcg ggaagtgtgg aattagcctg cgtcatatcc acagacagag cgcccagtgc
     2701 ccccatgttt ttcccgatac cgaaattaaa agcacgataa cgatccgcca gttgcgttcc
     2761 accatatatt gtccagccag ccggaaggcc gtggagtaat gtactttgga aaaagcgggg
     2821 tttttcctgc tgcgtatttc cactacggta ttctcctgcc gtaatggaat aacgagtatg
     2881 cccttcacgt tgcaaaagcg ggactgacga atagggtacg gtaaaaatct gcgtgctgcc
     2941 gtcagcctct ttgatcgtta cctgcaagtc accactatta cctgcggcat agatatcgtt
     3001 gatggtaaaa ggccccggtg gcaccgtact attataaatg tcatacccat tttgtttaat
     3061 agtgacctgt gcagtaccac gagcaatacc gtggatcacc ggggcaaatc ctctttggct
     3121 atcgggtaac atattgtcat ctgaggccaa ttgtgcgccg cgaaagttaa taccatcgaa
     3181 aatatcgccc tgggtataac catcacccag cgtcagccgg gaacgtaacg gtattatgtc
     3241 tcgctcaagc caggtattga tatgctgcca tttatttttg ctacctgatg atctgtcgct
     3301 actgttataa ctccagctgg tattgtcgcg taaacaccac gcaccaatat ttaacccact
     3361 ctgtaggttt aaatatgcat aatggctgtt acccccaatc tgattctgta cactatttcc
     3421 gctgaaatta taattgagca atccggcatt aataccggga tcccataact caggaggaat
     3481 ataaccacgc gcgcgattac tcataaatgc ctgagggatc gtcaggttca gtcgctgctg
     3541 accaacatcc agatgcgcag tagcgtcatg gatcattgag gttaatggca cgcaggcatc
     3601 atctgccagc agattcatac cggagacaga agccgcattc agccccatac tggcaagttg
     3661 cgcgcgtgtc aggcagggaa caatcccttg ttcactgtcg cccgtattaa atgtgacatc
     3721 acgcgttgcc atataaccat tattcaaata gatatcgacg cgatacgtcc ctggcggtaa
     3781 ttcttgccca ttttcaaaac gcgataaatc ggccacagcc tggggatcat ccgctaaaaa
     3841 gcgcggatta aaatagagtt cggcagatga caaaggtgcc tgtgcggcaa aagcacaggc
     3901 gacaacgagc cggacaaaaa aaccagccaa acgatgctta cgaatatgca agcattgtgt
     3961 gtttcgctgg taaagtctta aattcagata tgacattatt cctccggttt aaaattaccc
     4021 ggcagtcaat tctttttatt caggcgagaa attccccctg cgttattcca ttacgcccgt
     4081 cattttgggg gtaagtgcgc cataatcatt tattgttcgg taagtaatat tgcttcctgc
     4141 atcagaaggc aatttaaccg tgctttcgcc cattggaggc accaatgcat tttcaagaac
     4201 ccgggttccg gcattcaact ctgttaccgt cagataatag ggtgtcgggt taatcagcgt
     4261 cagagaattc gcgctacgac gaaatcttaa tttttctgcg gcctgatcgg gtggcaacgc
     4321 taatttagcc gggcgatagt acagtttaat gcggctgata attgcgaact gtagcgtatt
     4381 ctcagtcaat tttgatttat ccattgacgg aatcgcttta acattcatcc agaataaact
     4441 ttcccggtcc tgtggcaatt ggttatttgt tgcatcaaga atacgcaagg tattctcttt
     4501 ttttcccttc atcgcaaaca gaggaggcgt cacgataaaa cgaccatcct ttacaccatc
     4561 ggcattttcc atccatgatt gaattaaata ggtactattt tcatcattat ttgtcacggc
     4621 aagttgcact tgtttttgcc ctgccggata aattacgcga gtcgcaccta aggccactcc
     4681 cgcttcagcg cgtccggcaa ccatcattgc catgaacatc aggatacctg ccagcaagca
     4741 gaatgttatt tcctgcgatt tccttacatt ggcgttttta ttactcactg tcctgttcct
     4801 gttatcacat tatctgctga acaattactg ataggttaaa gagaaccagg cctgggcatt
     4861 ggcgatgccg ccagtaaccc aacgcccggt agcacgatat ttggcgatga aatgtagcga
     4921 agtagagcct gagtaaagcc gtttccagtt tactggagga cgattaatcg gtacgaggtt
     4981 tccttcatca tcaaacaacg ctacgccaat attggtggct atccctggcc cctctcccac
     5041 ggaaagcaca tccggatttt taccatccgc gacaccgtga aacgccacac ccacacgttc
     5101 actcaccacc gtgctacatt cccgtaaatg aataacaaaa ggcaccggtg cgctatcttc
     5161 ccctaccgca tgaaaccggt tactgctgat ttgccccata ttgaccgtca tttgtttatc
     5221 accggcttca atccggcaag tttccgcaat aatgacgccc tgaaattgca tatttccgcc
     5281 gggcagcgta gtattccatt tatttccggc cagagcaaac attggcagca acgacgctaa
     5341 tagaaataat ctcttccttt tcatcgttat ttttatcgca caagggtggg catccctgcc
     5401 cgtaatgacg tccctgaacc tgggtaggtt attgatactg aaccttgaag gtcgcatccg
     5461 cattagctgc gccaggggtt gcgacaccgg ttgcataata acgcgcctgg aacggaatgg
     5521 tgttagtacc gttattcagg gttgtttgct cactgaatgt cgcaccatcc agcgtcagcg
     5581 cagcacccgt tctgtccagg atctgtacac caacgtttgt tgcgctaccc gcagctgaac
     5641 tctgcagagc cagaacgttg gtatgacccg catcaatcgc cgtacctaaa aaggcaactg
     5701 cggctttaga tgcaacattg gtatcgcaat cattcagctg aatgttaaaa ccgacagcag
     5761 aactggttgc tccttcctgt gccagcgatg cggtacgaac ctgtcctaac tgaacgattt
     5821 gatcaacaga gcctgcatca actgcgcaag cggcgttaac aacttcccct ttaaagtgaa
     5881 cggtcccacc attaaccgtc gtggcagcgg ccagagccgc tgtagaactg agggacagag
     5941 ccgacagaac aacgattgcc agagttttaa ttttcatgct gctttccttt aaaaaaacta
     6001 tttctaaatc gacatgggca gtcgttctgt acactttgtt ttgtcaacga gtttgactgc
     6061 caacactgca cagttttccc ccaaaagatg aaacatttgg ggccattttg actcatagag
     6121 gaaagcatcg cgcacaaact ttttcagttt atttgttggc ttaatattct attgttatct
     6181 ttatttatag atttttatat tgcatgaggt ggtttttgga gagaagaatg aggaagatgc
     6241 gtcgagccac agaaacgtta gctttacata tagcggaggt gatgtgaaat taatttacaa
     6301 gagaaataat ttacatatca aacagttaga tgatttttgt cgttttttaa aatttttatg
     6361 cttgagaaaa aatacgtaac ttatttatga tatggacaat ttggccccaa ttgtcttgta
     6421 tttatttatt ttttaacttt attatcaata agttaattca aacctcttct ctttttaatt
     6481 tttcgtttat gagattattt ctttcccata atccggcaaa acgagcagca ttactggcgg
     6541 tataacgtac agtatggcga atatttcgat gcccgagata atcctgaatt aaacgagtat
     6601 ctgcaccacg ctccgccagt tcataaccgc aagcatgcct taacatatga ggatgagtct
     6661 gcgttacggt tccagcttca ataccggcat cacgaataat gcgataggcc tgctggcgag
     6721 aaagccgact cccgcggcga gaaataaata tggcgtcggt ccggtcagcg cctttccagt
     6781 tagcacgttc cagggtccag cgttccacag cttcacgctc atcaaaacgt aacgggtgaa
     6841 cggtagaaaa tccgttcttc agtcggcgaa tatttattcg accttcatta aggtcaagat
     6901 cctgataatg cagatcaagc agttcactaa tacgcatccc atgccgatat gccaacagaa
     6961 taagacaata atctctggct cccgttgccc cgtaacaaac cgcctgcatc atggcctgaa
     7021 cttctttacc ggtaagataa cgacgtttac tcacaatagt agtactccta actgagatat
     7081 atttgaatga atacctatag gaaacctcaa tcggtcaaaa ttagcccaat agataaaaag
     7141 ataaccacac acgaagtgat gtggctatca gtcaattaca ttaaatatca tacaaaaata
     7201 aaatatcact gatgtgacaa catctatttt cattgatttg attcaatata acaatagatt
     7261 gtagtagatt agcttccagt tgcttaaaaa ataaagaaaa aagtgcgaaa acaggagaat
     7321 ttaagtgatg tgtaaattta tgaatgattt tgtttattta ttttgaagaa cataaaccat
     7381 tgtattttgt atggatatgg ttaacttaat gcttattatg ataattaaga attatactta
     7441 caataacatc aattaataaa tggttaatca tttttgatat atcgtaagaa taatgtagtt
     7501 tttaacacca tccctggtat ctcaactatc tctataaaac agcgtgacgc tgtcgtcctc
     7561 tggctctatc ccagatgccg taaaaacgcc ctgcattgct ggcggtatac cagacagtat
     7621 gacgaatatt gcgatgccca agataatcct ggataagtcg cgtatctatt cccatattcg
     7681 ccaaagcaaa accacacgaa tggcgtaaca tgtgcggatg aatctccagt gacaacccgg
     7741 cattaccacc ggaagtcgag ataatatggt aaaactgttg ccgagaaagc ggattcccct
     7801 tacgcgataa aaatacccac tcgctctcag catgcgggta cgaagtacgg atactcaacc
     7861 agttttttaa agcctgaact tctttattca atagcggggg cgttgttgaa aagccttttt
     7921 ttaatcgatg gatatatata cactttgcct taagatcaat atccgaaatc ctcaatcgac
     7981 aaatttcact cgcccggaaa ccatgaataa aacaaagcaa agtcagacaa taattacgtg
     8041 ctgcatgagg cccggtattt gctgctttaa ggagtgattc gatttcacta tgggtcagga
     8101 agttcctttt tttgttatca gccttattct tcatcgtttt tcccttataa ttacagacgc
     8161 gcactagctg tgctgggtta gcaataatcc aacattttta tcctgattat gtttataaaa
     8221 gcgaacgttt gcttaataac taacgtaagt ggaccagttc ttctgagtga acttaaatgg
     8281 ggtagcaaca gttaattata ataacgttgc catacaaacg ctatgctctt gcatgctatg
     8341 tacctttata tatttatcaa tcggatacga atctaataat acccctcatc attaatgcaa
     8401 ctaatcttat ctatatatca tgtgatatgt tctgtaacaa gtaaaatcag cataaaaaat
     8461 tcagcaaaat ttaatattcg ttcaactagc cgtctcacat atgacattat tagtcaactc
     8521 catttgccac aaaatggcta tttcatggaa cgcagtaatt tcttagtggc tattatcatg
     8581 ctaattctta ggtccctagc gattattcct ggctgattcg tcagcttacg ccatgagatt
     8641 gttacattcc tttttatcct ggagatatgg gggaacgcag gagatgattt tttcatctac
     8701 attcgttggt aaatatagct ttggtcacta cgacttaaac cttcctcaat cacagccatt
     8761 cgccgggcga tacggcaaac aaactaagtg aaacatcatc ctgaaaacac caacatcaac
     8821 aagcctctcc agatcgactt cagaagtgac cagttacaag ccacaaacca ggaaacatat
     8881 ttcatgatga gaattatgct cagcctgatg gcgggtaaac actgattttc accaagctca
     8941 ctgagttagc ttgcaaagct tcccttgttt agactccaaa taaattattt ataaaacaaa
     9001 catttaaaca aagatcttta gtttttgaag accaccgcaa gtgttcgtct ggcttcacat
     9061 ggcatcttcc tcttagaaaa agatcgacat attttgtgac acgaattgca aatctggttt
     9121 tgttgtatgg attgcgtgat ttttgatctg gtataacagg tataaaggtg caccaagata
     9181 gtcaatgaga cagggcatct cgcaatctat ggcaaacatc acttcagttc tttctcatcg
     9241 ggtgatgaaa acgcacttca gtctgaaagg aatatgaaaa tgagatcaac agacactcta
     9301 ttttatgact ctgggtaaaa tggattgagt aagtgatata gcttacgaaa attcaaatca
     9361 attaaacatc agaagagatt ttatactcag gtatttaatc tggatctctg tttatttaaa
     9421 taatgtgaaa agagattttt cacaggagac cttatacaaa aaaatataaa atacagctac
     9481 cggttgccaa agacactata agcctggcaa aaaatattac acaacataaa tgctaattgt
     9541 ttatgcgggc tttgtattgc tttctgtatc ctacaaatga gtgaaattta tgaaaaaggc
     9601 taaaatactt tctggcgtat tattactgtg cttttcgtcc ccattaattt ctcaggctgc
     9661 gacactggac gtacgtggtg gatatcgtag tggaagccac gcctatgaga ctcgactcaa
     9721 agtcagtgag ggatggcaaa atggatggtg ggcaagcatg gaaagtaata cctggaatac
     9781 cattcatgat aataaaaagg aaaatgccgc actcaatgat gttcaggttg aagttaatta
     9841 cgcgattaaa cttgatgatc aatggacggt gcgcccggga atgttaacgc attttagcag
     9901 caacggcaca cgctacggac cctacgtaaa actgtcctgg gacgcgacaa aagatcttaa
     9961 ttttggcatt cgctatcgtt acgactggaa agcttaccga caacaagact tatccagtga
    10021 tatgtctcgt gataacgttc atcgttggga tggatatgtc acttaccata ttaatagtga
    10081 tttcaccttc gcatggcaaa cgacgctata cagcaaacag aacgattatc gctatgcaaa
    10141 ccataagaaa tgggcgacgg aaaatgcatt tgttctacaa taccatatga cgcccgatat
    10201 tacgccatac atagaatatg actaccttga ccgtcagggt gtttacaacg gcagagataa
    10261 tttatcggaa aacagttatc gcattggtgt gtcatttaaa ctgtagtaga caggagacag
    10321 tcacaatgaa taaaacaata acggcgcttg ctatcatgat ggcttcattt gccgcaaacg
    10381 cgtctgtatt accggaaact cctgtgccat ttaaaagtgg taccggagca attgataacg
    10441 acactgtcta cattggttta ggtagcgcag gtacggcatg gtacaagctg gatacacagg
    10501 ccaaagataa aaaatggaca gcgttagctg cattccctgg tggaccaaga gatcaagcaa
    10561 cctctgcatt tattgatggc aatctgtatg tgtttggcgg cattggcaaa aacagagagg
    10621 gcttgactca ggtatttaat gacgtacaca aatacaaccc caaaaccaat agctgggtta
    10681 aattgatgtc gcacgcaccg atgggcatgg cgggtcatgt gacttttgta cacaacggca
    10741 aggcttatgt tactggcggt gttaaccaga atatcttcaa tggctatttt gaagatctca
    10801 acgaggctgg aaaagattca accgctgtag ataaaatcaa tgcctactat tttgacaaaa
    10861 aagcagaaga ttatttcttc aataagtttc tgttgtcttt tgatccctca acacagcaat
    10921 ggagttacgc tggcgaatcg ccctggtacg gaacggctgg tgcggcggtt gtgaataaag
    10981 gtgataaaac ctggcttatt aatggcgaag ccaaacccgg attgcgaacg gatgccgtat
    11041 ttgaacttga tttcaccggt aataatttaa aatggaataa gcttgctccc gtctcatcac
    11101 cagatggcgt cgctggcggt tttgcgggga taagcaatga ttctcttata tttgccggag
    11161 gggccggatt caaaggttca cgagaaaatt accagcacgg aaagaactat gcgcatgaag
    11221 gcctgaaaaa atcatatagc actgatattc atctttggca taacgggaaa tgggataaat
    11281 cgggtgaatt atcgcaaggt cgggcctacg gagtatcatt gccctggaat aatagtctat
    11341 tgattattgg cggtgaaact gcaggcggca aagcggtgac ggattcagtt ttgatctctg
    11401 tgaaggataa taaagtcaca gtacaaaact aacgcttcag ggccccagta aaggggccct
    11461 gctatcaatt tatcatttca attacgcgaa aattatatgg acgcaataat atcgcccgat
    11521 tattactatg ttcttaccat tgctggtcag tctaatgcta tggcgtatgg cgaaggactg
    11581 ccattaccgg ccagggaaga tgcgcctcat cccagaatta aacaattagc gagatttgcg
    11641 catacgcatc ccggaggccc gtcatgtcac tttaacgaca ttattccact aactcactgc
    11701 ccacacgatg ttcaggatat gcagggttat caccatcctc tggcaacgaa tcatcaaaca
    11761 cagtacggca ccgttggcca ggcactgcat attgcacgga aattactgtc ctttattcct
    11821 gataacgcag gggttctcat cgttccgtgt tgccgtggcg gatcggcttt taccgcgggc
    11881 agcgaaggga catattcaga acggcacgga gccagccatg atgcttgtcg ttggggaacg
    11941 gatactccgc tataccagga tttagtcagc agaacgcgag ccgcactggc aaaaaatccg
    12001 cagaacaaat tcctcggcgt atgctggatg caaggcgaat ttgacttaat gaccagtgac
    12061 tacgcgtcac accctcaaca ctttaatcat atggttgaag cctttcgtag ggatctaaaa
    12121 caataccatt ctcagcttaa taatattact gacgcgccgt ggttttgcgg cgataccacc
    12181 tggtactgga aagaaaattt ccctcatgcg tatgaagcta tttatggcaa ttatcaaaat
    12241 aatgttttag ctaatattat tttcgtcgac ttccagcaac aaggtgaaag aggactgacg
    12301 aacgcgcctg atgaagaccc ggacgattta atcatgggat attacggttc agcgtaccgc
    12361 tcaccggaga actggacgac ggcactgcga agcagtcatt tcagcgcggc agcccgtcgg
    12421 gggattattt ctgacaggtt tgtagaagca attttgcagt tttggcgcga aaagtgagcg
    12481 ttcattaatt tgatttattc gcatccatca acgcttttaa ctcctccggc gtaaacggtg
    12541 gtttgcgtcg gtgcaacatc gcatggcaat tagggcacac aagcagcaaa aaaacaaatg
    12601 agatcaaggt aatatatctt cacttgcagg cttgaacagc agaaaatttg cgagcatgat
    12661 cgcaattatt tcactgctag ggatatcaat ggcttccagt aaatcgctac agcaggcaat
    12721 tgccaatata aaaatctggc ataagggtga acagcgcgca ccgcataaac cattgttatt
    12781 gctatacgta ttagcgggat acctgaatgg acatccacgc cttttcgatt atggatcgga
    12841 aatctacgaa cctctgcaca gtttactgga acgttttggg ccacagcgtt cacagtaccg
    12901 acctgatatg ccgttctggc gattacaagg cgacggattc tggcaattac acaatgccga
    12961 gctttgctca acagcaggga gcagccgaca accgccagtg aaggaactga acgaatacca
    13021 cgttgcaggc ggtttcgatg aacagcacta cgccctggta accggcaata aaaaactcat
    13081 caatactcta gcgcaacaga tactcgaagc gcacttcacc gagagtattc aggaagaaat
    13141 tgctgacgag ctgggttttg atctccagca aatccgtaaa cagcgcgacc cgttatttcg
    13201 caaaaatgta ttacgcgctt ataactacca gtgcgcgata tgtggcttta atatgcgcca
    13261 cgacgacacc accgtcgcgc tggaagcggc gcatataaaa tggaaacagc acggcggtcc
    13321 ttgtgaaatt cctaatggcc tggcgctttg cgcaattcac cataaagcct ttgataaagg
    13381 atcgattggc ctggacgaaa atatgcgggt actggtatca gatgcagtaa acggcggagg
    13441 gagcgttgag agattattct gggattttga cgggaaaatt attgcgttgc cgcaggtgcg
    13501 taaaaattac ccttatgagg ggtttgtgga gtggcatcgg aatgaggttt ttagggggta
    13561 aggttgataa ctatcgcagt atgttcaata ttgctgacat aatcagtgca ggatcttgcc
    13621 gctagtttac aactcgcctt cgatagcggc gcaaaacgag ttctgttgcc gatatcctgg
    13681 gctatggata ttccaacagt tccggcagag ttgtttacca agtttcaggt aagtttttac
    13741 tcagaaccgg ttgatgcggt ttataaggcg ctggatgtga attaacacat tcctcagctt
    13801 atttacaaaa ggattattcc catttgatat aatatcagtg ccaatttact tttttcaatg
    13861 gaatatatct cggctaaata acaataaaac ttatcgctaa tggattaaat aaacaatttt
    13921 c
//
LOCUS       DAJTSL010000101        13720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTSL010000101 DAJTSL010000000
VERSION     DAJTSL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.101"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(89..775)
                     /locus_tag="OO433_002732"
     CDS             complement(89..775)
                     /locus_tag="OO433_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6051148.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1356..2144)
                     /gene="phoH"
                     /locus_tag="OO433_002733"
     CDS             complement(1356..2144)
                     /gene="phoH"
                     /locus_tag="OO433_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6051149.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2766..4037)
                     /gene="efeB"
                     /locus_tag="OO433_002734"
     CDS             complement(2766..4037)
                     /gene="efeB"
                     /locus_tag="OO433_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6051150.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(4043..5170)
                     /gene="efeO"
                     /locus_tag="OO433_002735"
     CDS             complement(4043..5170)
                     /gene="efeO"
                     /locus_tag="OO433_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6051151.1"
                     /translation="MTINFCRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITAQAEDLAQLRGVLGLD"
     gene            complement(5228..6056)
                     /locus_tag="OO433_002736"
                     /pseudo
     CDS             complement(5228..6056)
                     /locus_tag="OO433_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
     gene            complement(6725..8233)
                     /gene="putP"
                     /locus_tag="OO433_002737"
     CDS             complement(6725..8233)
                     /gene="putP"
                     /locus_tag="OO433_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR6051152.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(8392..8601)
                     /locus_tag="OO433_002738"
     CDS             complement(8392..8601)
                     /locus_tag="OO433_002738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051153.1"
                     /translation="MMLQFCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            8656..12618
                     /gene="putA"
                     /locus_tag="OO433_002739"
     CDS             8656..12618
                     /gene="putA"
                     /locus_tag="OO433_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6051154.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNCNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTYETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQCNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGIQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(12658..13296)
                     /gene="rutR"
                     /locus_tag="OO433_002740"
     CDS             complement(12658..13296)
                     /gene="rutR"
                     /locus_tag="OO433_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6051155.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            13584..>13720
                     /locus_tag="OO433_002741"
     CDS             13584..>13720
                     /locus_tag="OO433_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR6051156.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     AL"
BASE COUNT         3405 a         3733 c         3377 g         3205 t
ORIGIN      
        1 tttaaagaag agctcgtacg aaggtacggg ctttttttcg tatattgcac acaccggggg
       61 gatgagaagc cccgaccggg atacttagtt aatgcaccgg ttcactcttt gtcgccgcga
      121 ctaatgggcg aattttgtca cgagtctccc agaccggagg aatttcctcc aatttagtga
      181 cagaagctcg tacaaaaggc acccgtgcaa gagtcataat tgtatccaac caacggtcac
      241 gaataatgcg atccgccctt tcatgactcg ggtcatccag ctcaataaca cagactattt
      301 tattgttctt ctttgaaacc agaacataat ccattgccat gccattataa attgcaaaga
      361 aacgctttct ttcttcttcg ctaaactcag gttgttctgg aaagttgatc aagcgcccca
      421 gagagacctg gcaatggata tgacaataat gtccaaacca tttttccaga ttatcgaaat
      481 acattttttc acaacgagtg attaaccact cttttctttc caataaaata ccttcggcaa
      541 ccattccctg aaataactca tctcgctctt gccaacgaga gtcacgatag gttttctgcg
      601 gtatatagct ccttgtcgac gccttagcgg caggggaagt tgcagatgaa gggacgggtt
      661 gcgatttagc gatgattacc gatgaggcaa cttttcggag tgtcaatttt ctggcaacag
      721 caaaaccggc gataaatgca attgtcaaac aagttacaaa agccataaag ctcataatta
      781 attttcacct atccatgttg attatatttc attaccaatg ttactcgctg cagcgtgtta
      841 catgccaaca atgcaacgat caatatatag ttagtatcat gttttgatta ttacaaccat
      901 ccctatttag ccattgcggc ttgccttcca caaaaaaata acaacacaca atattagcaa
      961 agaattaatt aagattaacc ttacatttat aatattatcc ctaaagaata aatcagagga
     1021 atatattcgg tgttaaacag tcagtcaaag tatagcccat tgttgattaa acctcataag
     1081 caaaattggc accagatctc ctgtacaggt tgaatggccg aaaaaagata ggcagatgct
     1141 catttttttg gcttctggaa gaaaaactct ccgcgctggc agtattacac tggtctgatg
     1201 ctatgacgaa aaatgcctgg taagagtatc taaaccagac tccagttcag cttatcaaca
     1261 cgctcaaagt cgcccccctg aacgctaacc tgaagtgaaa atctgttgaa ataaaacaaa
     1321 gcccggttcg cccgggctct gcaccgataa cacacttaac tgtatgcatg cagcgtacgt
     1381 tggcaaagtg ccgaacgtac gcaatcctct tttccgaacc ggacgatccc aaccatttca
     1441 tcttcttcga aacgttccag cgcgtcactt aatccggagc acacgccgcg aggcaaatcg
     1501 cattgcgtga tatcaccgtt gacgataacc gtcacgttct ccccgaggcg ggttaaaaac
     1561 attttcattt gcgcggcagt cacattctgc gcctcgtcaa gaatgacgac tgcattttca
     1621 aaggtacgtc cacgcatata ggcgaacggc gcgatttcca ccttccctat ttccggtcgc
     1681 aggcagtact gcataaagga agcccctaag cgccggacca gcacgtcgta gaccgggcga
     1741 aaatagggag caaacttttc tgcgatatca ccaggtaaga agccaagatc ttcatcggct
     1801 tgcagaactg gacgggtgac gataatcctg tcgacatcct tatgtatcag ggcctctgcc
     1861 gcttttgctg cgctgatcca ggtttttccg cacccggctt cgcccgtggc gaatatcagc
     1921 tgcttactct caatagcctt cagatagtgc aattgcgctt catttcgcgc gaggatgggc
     1981 gaggtatcgc gactgtcgcg ggccatacca atggcttcta cgccgctcat ctgcacaagc
     2041 gaggtgaccg attcttcttc acgctgctta tggctgcgcg aatcccgtct cagcacacgt
     2101 tttgcctcgc gacgagcttt gatcactgct ttttgtcttc ccatggagag caccttgagt
     2161 tgtttgtatt catcacacgc gccgttggca gcgcgattat gcgcacgaac atcagagggt
     2221 tggcttcctt gtaagccata gtttgctttt ggataaaatg ccgaaaaacg gctacgcgca
     2281 ccgtttacgg cgtcggtaac acatgaaaag aaaggatgag gttgaaaatg caaagtgacg
     2341 agatgactac cggaggagaa aactccgcga gtggtggcgc gttgattatc taaaacatgt
     2401 ccagtacagg acgttaccat ccgcgatctc catagtgact gactatcact gccgggaact
     2461 tccgctgcta cttaataagt acaacagatc tcgcatttta ttgcaacaat atatttactt
     2521 atatttaact ataaaacact atttcagtga cattagtttc tactggaaag atgacagagt
     2581 gatgacagtg atgaaaaaag ctgtgtgctt tcagcaggat ttgcatcagg catcagtgca
     2641 cgatcgccgg atgcggcgtg aacgccttat ccagcctacc aaacccgcac tgattgtagg
     2701 catgataaga cgcgcaagcg tcgcatcagg catctgcgca caaatgcctt atccgcctaa
     2761 aaacattaaa cccgcaataa cgcgcttccg agataatcgt tcgcgtcctt cactcccggc
     2821 agcgaaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     2881 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc
     2941 aaccccatat ccagttgtcc ggagttggtg acccccaacg aataactgta gccgcgacgc
     3001 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     3061 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     3121 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     3181 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     3241 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     3301 ttatcattct ggctatcggg attggcagtg ccgtctttaa agcccagcaa attaatcggc
     3361 gtctctttgc ctttactacg cgctgcgtga tcggaaataa acccttcccg cttccagcgc
     3421 acgctgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggat caccgtgtcc
     3481 tgggtgttgg cgcaaatttg tagcaacaca tcaccatggc ataacgccgc atccagcgag
     3541 tcgttgggga aacgcgtcat cttctgcaac tttttcggca tctgtggcgc aaggccaaac
     3601 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     3661 tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgcc
     3721 gctccgccct gagtcagaaa agcaaaacgc tgagtcaaca agcgaaacaa ccgttcaaga
     3781 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     3841 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     3901 gaaagcgtac ccggcgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     3961 agcgccagcg cacctatcac tttcagtaaa cgtcggcgtg acggttcatt cacgccgttt
     4021 tcatctttat actgcataac gcttaatcca gtcccagcac accgcgaagt tgcgccagat
     4081 cttccgcctg cgcagtaatc ggtcctttca gtgcattccg gtcggcatcg gtcaatttgt
     4141 cgtaggtttc aaaaccgtct ttagtacggt atttcgccag aatggtatcg acctttttaa
     4201 agttggcatc gacttttgcc agcagttccg ggttggcttt ttgcagttgt ggacgcagca
     4261 aatcgacaat tttctgcgag ccttcaacgt tagcctggaa atcccacaga tcggtgtggc
     4321 tgtagcgatc ttcttcaccg ctaattttgc tggctgccac ttcctcaatc agtccggctg
     4381 cgccgccgac cacttttgaa ggtgggaaag ccagttcact gatgcgtttt tgcaaatcga
     4441 ccacatctgt ataaagctgc tcagcgtact gatccatccc tttggtggtg ttgtcgccaa
     4501 acaatgcttt ttccagacgg tggaaaccag tgaattttgg gtcggcggct ttttgctcgt
     4561 aatcatcttc acgggcgtca atgctgccat ccagatcgga gaacagttca gcaatcggtt
     4621 caatgcgctc atagtgctgg cgcgtcggcg catacagtgc tttcgctttt tcgatatcgc
     4681 cagctttaat cgcgtcggta aaggctttgg tgtcggtcac cagctgcgtg gtttccgcca
     4741 tgacatacgc tttatatgca gtaattgcac caccaagact taacagcgca tcactttgcg
     4801 ccgcatccgc cgttgcctca cctttgacga tcaacttccc tttcgggtta gtcagcagac
     4861 cgcaggtcat atcgtattcg ccaggctgta aattcgccgt cattttctgg ctaaaaccag
     4921 gggcgatatt ttcccgctct tccaccacca tcacgccttt gaggatctcc cactccagcg
     4981 ccttctggct atggttctga ataatgaact gtgttttccc ggcgttaacc gtaatggtca
     5041 tcggttcgca ctgcttatcc gtcacggtca ctttgacctg cggcacatca gcggcgttag
     5101 ccataaaagc agaagaaaac agcgcagcca cgctcaactg caatgcgtta cggcagaagt
     5161 taatggtcat gacgagacta tccctttaaa gtatgttgta actaagcaag agtttgcgtc
     5221 gtatttgtta cgcggagcga gacgctgtcg cccctgcgcg tggtggcata gcaaatgcca
     5281 ccagcgccgg gatgagataa ataaaccaga cggcgacttc gctgacgctc ggcgcttcct
     5341 gatagccaaa aatcccttcc atcagcgtgc caaacagcga gtgagttgag agcaccgcac
     5401 tcatatcgaa ggcgatttcc tgaaagtggt tccacaatcc ggcttcatga aatgcgcgaa
     5461 tggcaccagc tgccagccct gcggcgacga agagaataaa caggctggtc catttaaaaa
     5521 atgcaccaag attgaggcga ataccgcccc agtagagcag aaagccgagc accacggctg
     5581 tagcaagacc gagcattgca cccagcggcg gccagatccc gacatcttgt tgaaatgccg
     5641 ccagcaggaa aaagaccgac tccagccctt cccttgcaac ggcaaaaaag accatcatca
     5701 ccagcgccca gccatgatga tttccacgct acaatgcgct atcgactgcc tgttccagtt
     5761 gcacttacgt tgcgcgacac tttgcgcatc cagaaaacca tccaggtaag gatcaccacg
     5821 gcgatcaccg ccacgatacc ttcaaacagt tcctgttctt tttgcggaaa ttcgccggtg
     5881 gtttcgttaa tgaagatccc caggcccagg cacaacgcag cggcaagcaa cacgccaatc
     5941 cacatcacac caatccatcg gcctcgctgg gtacgcttaa gatagctggc aatcaaactg
     6001 acaatcagcg cggcttcaag tccttcgcgc aacataatga gaaacggaac aaacatgcca
     6061 cccaccctta aacgtttttc tgagtcaatc tatgcaaaga aaagtaaatc gcagtgatag
     6121 cgattatcat tacggtaaag aaaaatacaa gcggaatgtt tataaaatga tgttggaatg
     6181 taaacaaaga gaggtaatgc caggggatat aaacgattat tccagggtag aaagatggga
     6241 ttgtttgcaa agaaccatgt gttcttgccg gatgccgcgt aaacgccata tccagccgac
     6301 aaaactgtcc agactcaatg catcagagct tccatgtaaa actgataagc gggtgtatca
     6361 agcaatttcg tccatccgcg attatcaaat ctaacttttt gcccggaaca taataacagc
     6421 tcgggtgttg gtggcgtcaa aatcgctgaa gcgttccccg aaggccggga cagcccacat
     6481 ggatgtgggc tgagggcgcg tcttacagga cgttacctcg cgcccgaccc ggtagccgaa
     6541 ggggataagc tgaaggtacc gcgcagcggc gattttgttc gccagagccc gggggtgcac
     6601 agggggcggc ggcgattggc cgccccctgc gcgctccctg caccattcaa tttatatggc
     6661 gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc gcaggctaag
     6721 tcccttagct ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca tcggcctcgg
     6781 caaagcgttt ttgcatcgcc gctgacggcg ctttacccag caaactaaac actacaatcc
     6841 cgatactgcc gaagatgaag cccggaataa tttcgtacag tcccagccag ccgaactgct
     6901 tccagacgat aaccgtcagc gcaccgatga tcatccccgc cagtgcaccg ttacgcgtca
     6961 tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca aagcctgccc
     7021 acgcgtagct cactaagccc agcacgcggt tttccgggtt tgccgccagc gcaatcgcca
     7081 ccagcgccac caccagcacc atcacacgcc ctacccacac cagctctttc tggctggcat
     7141 gtttacgcag aaacgctttg tataaatctt cggtaatcgc actggagcac accagcagtt
     7201 ggcaacttaa ggttgacatt accgccgcca gaattgccga cagcagaatc ccggcaatcc
     7261 acgggttaaa cagaatttgc gccagttcga taaacacgcg ctcggcgttc tggtttaccg
     7321 cgcctgctac cgccgggtgc tcattaaagt aagcaatccc aaagaagccg acagccaccg
     7381 cccctgccag gcagaggatc atccaggtca tactgatacg acgcgcatgg acaatgctgt
     7441 ggtgagaatc cgccgccata aaacgcgcca gaatgtgcgg ctgcccgaag taacccagcc
     7501 cccaacccat cagtgagata atggcaacaa agttcagacc tttgagcata tcaacgtttt
     7561 cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca ctgataatga
     7621 cgataaccgg tgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca gtgtcagtcc
     7681 agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgccgca gctcccgccc
     7741 acagagccgt ttcgtagctc atgccaaagg tactttcaaa cagacgcgcg cctgccacaa
     7801 tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac cagcgcggag ataatgcgca
     7861 aaatgcggct tttatcttca aaacgcccgg tgaaataatc cggcaatgtt aaggcgttat
     7921 tattgtattc ggtatgcaca cgcaaccgcc cggccaccag cttccagttg atccacgcgc
     7981 ctaatgtcag gccaatggcg atccagcttt cggaaatccc ggaaagaaaa accgcgccag
     8041 gcaaccccat taacagccag ccgctcatat ccgacgcacc cgccgataat gccgtcacaa
     8101 atggcccaag actacgaccg cccagaatat agtcgtcaaa gtttttcgtt gatcgccagg
     8161 cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc aacatcggtg
     8221 tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa acttgccgtt
     8281 atatctgcaa ccggaacggg gtaacagagt ttatttttta ccagggcgac cgtatcctgc
     8341 cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat tttagtgctc
     8401 agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg cacctttcaa
     8461 aaatgttaac tgccgcagag aaaaagtctg agttattttt ttaatccctg tcatatcaat
     8521 ttcttttatt aacatttcat tcatttttaa gcttgctacg caggtcacat ttaacatggt
     8581 tgcacaaaat tgcaacatca tggatatttc acgataacgt taagttgcac ctttcagaac
     8641 aacaggagta atggcatggg aaccaccacc atgggggtta agctggacga cgcgacgcgt
     8701 gagcgtatta agtctgccgc gacacgtatc gatcgcacgc cacactggtt aattaagcag
     8761 gcgatttttt cttatctcga acaactggaa aacagcgata ctctgccaga gctacctgcg
     8821 ctgctttctg gtgctgccaa tgagagcgat gaagcaccga ctccggcaga ggaaccacac
     8881 cagccattcc tcgactttgc cgagcaaatt ttgccccagt cggtttcccg cgccgcgatc
     8941 accgcggcct atcgtcgccc ggaaaccgaa gcggtttcta tgctgctgga acaagcccgc
     9001 ctgccgcagc cagttgctga acaggcgcat aaactggcat atcaactggc cgataaactg
     9061 cgtaatcaaa aaaatgccag tggtcgcgca ggtatggtcc aagggttatt gcaggagttt
     9121 tcgctgtcat cgcaggaagg cgtggcgctg atgtgtctgg cggaagcgtt gttgcgtatt
     9181 cccgacaaag ccacccgcga cgcgttaatt cgcgacaaaa tcagcaactg taactggcag
     9241 tcacacattg gtcgtagccc gtcactgttt gttaatgccg ctacctgggg gctgttgttt
     9301 actggcaaac tggtttccac ccataacgaa gccagcctct cccgctcgct gaaccgcatt
     9361 atcggtaaaa gcggtgaacc gctgatccgc aaaggtgtgg atatggcgat gcgcctgatg
     9421 ggcgagcagt tcgtcacggg tgaaaccatc gcggaagcgt tagccaatgc ccgcaagctg
     9481 gaagagaaag gtttccgtta ctcttacgat atgctgggcg aagctgcact gactgccgca
     9541 gacgcacagg cgtatatggt ttcctatcag caggcgattc acgccattgg taaagcgtct
     9601 aacggtcgtg gcatctatga aggtccgggc atttcaatca agctgtcggc gctgcatccg
     9661 cgctacagcc gcgcccagta tgaccgggta atggaagagc tttacccgcg tctgaaatca
     9721 ctcaccctgc tggcgcgtca gtatgatatt ggtattaaca tcgatgccga agaggccgat
     9781 cgtctggaga tctccctcga tctgctggaa aatctctgtt tcgagccgga actggcaggc
     9841 tggaacggca tcggttttgt tattcaggct tatcaaaaac gctgcccgtt ggtgatcgat
     9901 tacctggttg atctcgccac ccgcagccgt cgccgtctga tgattcgcct ggtgaaaggc
     9961 gcgtactggg atagtgaaat caagcgtgcg cagatggacg gccttgaagg ctatccggtt
    10021 tatacccgca aggtgtatac cgacgtttct tatctcgcct gtgcgaaaaa gctgctggcg
    10081 gtgccgaatc taatctaccc gcagttcgcg acgcacaacg cccatacgct ggcagcgatt
    10141 tatcaactgg caggtcagaa ctactatccg ggtcagtacg agttccagtg cctgcatggt
    10201 atgggcgagc cactgtacga gcaggtcaca gggaaagttg ccgacggtaa acttaaccga
    10261 ccgtgtcgta tttatgcccc ggttggcaca tatgaaacgc tgctggcgta tctggtgcgt
    10321 cgcctgctgg aaaacggtgc taacacctcg tttgttaacc gtattgccga cacctctttg
    10381 ccactggatg aactggtcgc cgatccggtc actgctgtag aaaaactggc gcaacaggaa
    10441 gggcaaactg gattaccgca tccgaaaatt cccctgccgc gcgatcttta cggtcacggg
    10501 cgcgacaact cggcagggct ggatctcgct aacgaacacc gtctggcctc gctctcctct
    10561 gccctgctca atagtgcact gcaaaaatgg caggccttgc caatgctgga acaaccggta
    10621 gcggcaggtg agatgtcgcc cgttattaac cctgcggaac cgaaagatat tgtgggcttt
    10681 gtgcgtgaag ccacgccgcg tgaagtagaa caggcgctgg aaagtgcggt taataacgcg
    10741 ccaatctggt ttgccacgcc tccggctgaa cgcgcggcga ttttgcatcg cgctgccgtg
    10801 ctaatggaaa gccagatgca gcaactgatt ggtattctgg tgcgtgaagc cggaaaaacc
    10861 ttcagtaacg ctattgccga agtgcgcgaa gcggtcgatt ttctccacta ctacgccgga
    10921 caggtgcggg atgatttcgc taacgaaacc caccgtccat tagggcctgt ggtgtgtatc
    10981 agtccgtgga acttcccgct ggctattttc accgggcaga tcgctgccgc actggcggca
    11041 ggtaacagcg tgctggcaaa accggcagaa caaacgccgc tgattgccgc gcaagggatc
    11101 gccattttgc tggaagcggg tgtaccccct ggcgtggtgc aattgctgcc aggtcagggg
    11161 gaaaccgtgg gcgcgcaact gacgggtgat gatcgcgtgc gcggggtgat gtttaccggt
    11221 tcaaccgaag tcgctacgtt actgcagtgc aatatcgcca gccgcctgga cgctcagggt
    11281 cgccctattc cgctcatcgc tgaaaccggc ggcatgaacg cgatgattgt cgattcttca
    11341 gcactgaccg aacaagtcgt cgtggatgtg ctggcctcgg cgttcgacag tgcgggtcag
    11401 cgttgttcgg cactgcgcgt gctgtgcctg caagatgaga ttgccgacca cacgttgaaa
    11461 atgctgcgcg gtgcaatggc cgaatgccgg atgggtaatc cgggtcgcct gaccaccgat
    11521 atcggtccgg tgattgatag cgaagcgaaa gccaatattg agcgccatat tcagaccatg
    11581 cgtagcaaag gccgtccggt gttccaggcg gtgcgggaaa acagcgaaga tgcccgtgaa
    11641 tggcaaagcg gcacctttgt cgccccgacg ctgatcgaac tggatgactt tgccgaattg
    11701 cacaaagagg tctttggtcc ggtgctgcat gtggtgcgtt acaaccgtaa ccagctacca
    11761 gagctgatcg agcagattaa cgcttccggc tatggtctga cgcttggtgt ccatacgcgt
    11821 attgatgaaa ccatcgccca ggtcactggc tcggcccatg ttggtaacct gtatgttaac
    11881 cgtaatatgg tgggcgcagt ggttggtgtg cagccgttcg gcggcgaagg gttgtccggt
    11941 accgggccga aagcaggcgg tccgctctat ctctaccgtc tgctggcgaa tcgcccggaa
    12001 agtgcgctgg cagtgacgct cgcgcgtcag gatgcagagt atccggtcga tgcacagttg
    12061 aaagccgcat tgactcagcc gctaaatgca ctgcgggaat gggcagcaaa tcgtccagaa
    12121 ttgcaggcgt tatgtacgca atatggcgag ctggcgcagg caggaataca acgattgctg
    12181 ccggggccga cgggtgaacg caacacctgg acgctgctgc cgcgtgagcg cgtgttgtgt
    12241 attgccgatg atgagcagga tgcgctgact cagctcgccg ccgtgctggc ggtgggcagc
    12301 caggtactgt ggccggatga cgcgctgcat cgtcagctag taaaggcatt gccatcggca
    12361 gtcagcgaac gtattcaact ggcgaaagcg gaaaatataa ccgctcaacc ctttgatgcg
    12421 gtgatcttcc acggtgattc ggatcagctt cgcgcattgt gtgaagccgt tgctgcgcgg
    12481 gatggagcaa ttgtttcggt gcagggtttt gcccgtggcg aaagcaatat ccttctggaa
    12541 cggctgtata tcgaacgttc gctgagtgtg aataccgctg ccgctggcgg taacgccagt
    12601 ttaatgacga tcggttaaac aggtagccgg aggatgttac accctcctcc ggcatcttta
    12661 acgtggtcga atcccctcaa taataatccg ctgcacgttt tcaaccgttt gattgaaaaa
    12721 tacctcatcg cgcaacgttg cgcctgtcac cgcctccacc tgaggggcga aatcggcgta
    12781 atgttgagtg gaagcccaaa tcataaaaat caaatgctgc ggatcaatcg gcgcgagttt
    12841 gccgcttttg acccaaccgg caatcagcgc cgatttctca tcaattaatg ccttcaaatc
    12901 gcccgtcagt tcatccatta acagcggcgc gcctgccagc atctccatac agaacaggcg
    12961 cgaagcctgc ggataatcgc gtgagacttc cagcttcaga cggatgtact ctttgatcgc
    13021 cgccagcggg gcgaaatctt cacgaaacgc ttttaacggt gccagccaga tatcgagaat
    13081 ctgccgcagc acggcaatat acagcgcctc ttttgacgga aagtaataca gcagattggt
    13141 ttttgaaaca cccgccaact ctgcgatctg ctccagcctt gtgccgtgaa aaccgaattg
    13201 tgaaaaagtg tccagtgctg cgctaagaat cgcttttttc ttcgcgctta ctgcgcgcga
    13261 acgtttaccc gttgttttca ctgcgccttg cgtcatgcgc tctcccctct tgtagattgg
    13321 caacaggata gcaaaacccc cgcccggcct cgaccttctg cactctcatc gcgcacggat
    13381 gcatgtttta tgtgcaactg ttttgaccgt ttagtccact ttttaccaga tattttagat
    13441 aacacaatgc taacacatta ataacattca ctttttaaaa actggcatcc gctttgcaaa
    13501 caagccaata cgcggctgaa aaggatatgc aggatgcagc gccccgtctg actttcactt
    13561 tacgagacga agagaggttg atgatgaaaa ttggcgtatt cgtacctatt ggcaacaacg
    13621 gctggctcat ttcgacccac gcgccgcagt acatgccgac ctttgaactg aataaagcca
    13681 ttgtgcaaaa agcggagcat taccatttcg atttcgccct
//
LOCUS       DAJTSL010000102        13577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTSL010000102 DAJTSL010000000
VERSION     DAJTSL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.102"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(231..1604)
                     /gene="cobA"
                     /locus_tag="OO433_002742"
     CDS             complement(231..1604)
                     /gene="cobA"
                     /locus_tag="OO433_002742"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6051157.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1623..2429)
                     /gene="nirC"
                     /locus_tag="OO433_002743"
     CDS             complement(1623..2429)
                     /gene="nirC"
                     /locus_tag="OO433_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6051158.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKSNRPVADKFNQTETAAG"
     gene            complement(2556..2882)
                     /gene="nirD"
                     /locus_tag="OO433_002744"
     CDS             complement(2556..2882)
                     /gene="nirD"
                     /locus_tag="OO433_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6051159.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2879..5422)
                     /gene="nirB"
                     /locus_tag="OO433_002745"
     CDS             complement(2879..5422)
                     /gene="nirB"
                     /locus_tag="OO433_002745"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6051160.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRIFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5684..6865)
                     /gene="tsgA"
                     /locus_tag="OO433_002746"
     CDS             complement(5684..6865)
                     /gene="tsgA"
                     /locus_tag="OO433_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6051161.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7136..7708
                     /gene="ppiA"
                     /locus_tag="OO433_002747"
                     /gene_synonym="rotA"
     CDS             7136..7708
                     /gene="ppiA"
                     /locus_tag="OO433_002747"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6051162.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGAPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            7813..7980
                     /locus_tag="OO433_002748"
     CDS             7813..7980
                     /locus_tag="OO433_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6051163.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            7970..8572
                     /locus_tag="OO433_002749"
     CDS             7970..8572
                     /locus_tag="OO433_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6051164.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            8604..9167
                     /gene="pabA"
                     /locus_tag="OO433_002750"
     CDS             8604..9167
                     /gene="pabA"
                     /locus_tag="OO433_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6051165.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            9253..10473
                     /gene="argD/dapC"
                     /locus_tag="OO433_002751"
     CDS             9253..10473
                     /gene="argD/dapC"
                     /locus_tag="OO433_002751"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6051166.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTASTPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(10540..12630)
                     /locus_tag="OO433_002752"
     CDS             complement(10540..12630)
                     /locus_tag="OO433_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6051167.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12681..13313)
                     /gene="crp"
                     /locus_tag="OO433_002753"
     CDS             complement(12681..13313)
                     /gene="crp"
                     /locus_tag="OO433_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6051168.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
BASE COUNT         3286 a         3492 c         3557 g         3242 t
ORIGIN      
        1 ttatatatta attaatattc attgattata tgaatataag cagactgctc gttaagaacg
       61 atacaggaat atttaattgg cgtcagtgag cgaatgactt aattttgtaa gtcgctgtaa
      121 cggtgaatat aacccattag cagataatat ttattaaggg gttatgtttt atttgctgaa
      181 tatataaaaa gagtcattaa gaaggccaga attctctggc ccgggttaaa ttaatggttg
      241 gagaaccagt tcagtttatc gcgcaggcca acaacccgac caataataat tagcgatgga
      301 ctgttcattt gctgagcaag ttcaccgagt tgcgtgagcg taccgtcaat cacgcgctgc
      361 gtgactgccg ttccgttttc gacaattgcc accggcattt cgccaggcat accgtgttca
      421 atcagctttt gctgaatagt cgcggcctga ttcaacccca tatagaacac cagcgtctgt
      481 ttttctgccg ccaggttttc ccagtccagc tcgccaccgg tttttaagtg tccggtaatt
      541 aagcgtacgc tctgggcata atcgcgatgc gtgagtggaa tacccgaata ggcagagcaa
      601 ccagaagctg cggtaatacc cggaaccacc gagaacggaa tacccgcgtt gcacagtgtt
      661 tccagctctt cgccaccacg gccaaaaata aacggatcgc cacctttcag ccgcaccacg
      721 cgtttgcctt tttgcgcttc ccgcagcagg atctggttaa tctcttcctg cggtacacag
      781 tggtatcccg cgcgtttgcc gacgaaaacg cgatcagcat cgcggcgtac cagattcata
      841 atatcgtcag aaaccagacg gtcgtagacc accacatctg cctgctgaat ttgttgcagt
      901 cctttcagcg tcagcagccc ggcatcgccc ggacctgcac caaccagcac cacttcaccg
      961 cgatggtcga gcggttcgtt gattaactgt tcggtcgttt cagtaatggc tttctgatcg
     1021 ttgtttgcca gcgactgcgc caggcggtcg ttaacgaaca atttctccca gaaacggcga
     1081 cgctcactca tcgtggtgaa ctgttgtttc actcgcccgc gtaattgccc ggcatatttc
     1141 gccacctggc ccagatgcaa cggcagcagt gattcgagtt tttcgcgcaa caggcgcgcc
     1201 agaaccggag aggtgccgcc agaggagacc gctaccatta gcggtgagcg gtcaataatc
     1261 gacggcataa taaagctggc ggctttcggc gcatcgacta cgttacagaa gatgcgacga
     1321 gcttcagcgg cttcgctgac gcgctggtta agcgtgtcat catccgtcgc tgcaatcgcc
     1381 agccagcagg tgtcgagaag gctttcatca aatggccctt cgacgagggt taacatgcct
     1441 gcatctgccc atgcggtgaa ctgtggaata aacgctaatg cattcaccgt taagcgagcg
     1501 cctgcgtcta acagcaacct tgctttgcgt tccgcgacat caccaccgcc gacaatcaga
     1561 cagtcgcgat cgcgtaattg gcaaaatata ggcaaatgat ccacgtaaaa accccttagt
     1621 aattaaccgg cagccgtttc agtttgatta aatttgtccg caaccggacg attcgatttc
     1681 ggcgtagcat accaataacc caatcccatg aatacggcac ctgataaagt attacccagc
     1741 gtcacccaca gcaggttatg accaataccc gccagcgtgt aggcttcgct gtggttgccg
     1801 aaccaggaga gcgcgaacag cgtcatgtta gcgatagagt gctcgtagcc ggacgcgata
     1861 aatgccagca gacaccacca gatagcgata aatttcgccg ccccttcagt gcgcagcgcc
     1921 atccagattg ccaggcaaac cagccagtta cacaatgcac ctttgaagaa gagtaccatt
     1981 gccggtgcag tggttttagc cagcgcgacg gagtgaacga tgctggtatc taccggcagc
     2041 aggctaccgc cgccccagct atagagcatg gcaacgaaga cggaaccgac caggttaccc
     2101 agccaggttt gcggcaggat tgcccacatt tgcccgtggc tgatgctgcc cgcttttacc
     2161 ccaaaggtga ggaacatggt gtgtccggtg aacagttcag aaccggcgat aatcaccagc
     2221 gttaaggcga taccaaaggt cgcgcccatc accagcggac gtacggacgg gtcgagcaaa
     2281 ttaccgagcg tgaaaatcaa gatgatccca agacccacat acgcgcccgc catggcggag
     2341 ctgacccaaa agccgagcgg gttatttgcc gacaggcgtg caatgcgcgc agcgttagcc
     2401 gcacacttat taatagtgtc tgtaaacatt tgattatcct ttaaaaaata aaaacaaaaa
     2461 aataaaacaa aaagtttaaa aaatgtcctg caatataccg aaggtaatta caggaccatg
     2521 caaaaagggg aggcattgcg cctcccgtta aaacattaac cgcgcagctg caccacgccg
     2581 tctttcactc gtgcttcgta atgtttgacg gaaaactgtt cgtcttccat gcacaagcca
     2641 tcacttaagc gaaaacgctg ttttttcagc gggctggcga cccacagctc gccctggtgt
     2701 tccgcaatca gcccgcgtga cagcacgctg gactcgaaga acggatcgat gttgctgatc
     2761 gcaaacacct ggtcgctgtg atacgggcgg aaaatcgcga cctgctcgtc gcctaacagc
     2821 gcgcagacgc cggtttcagg caggatgtca tcgattttgc agatgtcttt ccactggctc
     2881 atgcgttgtc ctccaccaga gttaccggga tacgttcata cggcgttgcc ggacggtgct
     2941 gttcgcgctc tggcaccatc tgcacgttcg ggtcacgctt gtcgctgttg atgaagtgtt
     3001 tgaagcgagt ctgcgcggac ggtgtattga ccgtttcagt ccactcacac actaccgctt
     3061 cacgcaggcg cgccatctct tcttccagat gagcgttcag ccccagtttg tcgtcaatga
     3121 tcaccgcttt caggtaatcg atgccgcctt cgaggttttc taaccacggc gcggtacgcg
     3181 tcagtttgtc ggcggtacgg atgtagaaca tcatgaagcg gtcgagatat ttgatcagcg
     3241 tttcgcgatc gatatcagct gccagcagat ccgcatgacg cggtttcatg ccgccgttgc
     3301 cgcaaacata caggttccag cctttttcag tggcgataat acccacgtct ttaccctgag
     3361 cttctgaaca ttcacgggta cagccggaga caccgaactt cattttgtgc ggcgtacgga
     3421 tgcctttgta gcggttttcc agttccacgc cgaggccgac gctgtcgcca acgccgtagc
     3481 ggcaccaggt gctacccacg caggttttcg ccatacgcag tgctttcgca taggcatgac
     3541 cggtttcgaa gccggcttca atcagctgac gccagatctc tggcagatcg tctttctgtg
     3601 cgccaaacat cgccaggcgc tgggaaccgg tgattttggt gtagagatta aattcacgcg
     3661 cgatacgacc cactgccatc agcccttccg gggtgatttc accgcccgga gaacgcggga
     3721 tcaccgagta ggtcccgtct ttctggatgt tagcgaggaa gttgtcgtta gaatcttgca
     3781 gcggagtatg ttctggcttc agaatgtatt cgttccagca ggaggccagc agcgaaccga
     3841 cggtcggttt acaaacttca caaccgtagc ctttgccgtg tttcgccagc agttcttcga
     3901 aggttttaat gccttcaacg cggatcaaat ggaacagttc ctgacgcgaa taagcaaagt
     3961 gttcgcacag gttgttgtta acttcgatgc cctgtttcgc cagttccgcg ttcagtacct
     4021 gagtgaccag cgggatacag ccaccgcagc cagtacccgc tttggtttca gctttcagcg
     4081 ccgcaactgt gtggcagcct ttgttgatgg cagcaatcag atcaccttta gtgacgtcga
     4141 agcaggagca gatttgcgcg ctgtccggca gtttatcaac accgatagac ggcttaccgc
     4201 tacccgagtg cgccggcaga atcagagaat ccgggttttc cggcagctcg atagcattca
     4261 gcaccagttg cagcaagtta ccgtagtcgc tggtatcgcc caccagtacc gcaccgagca
     4321 gggttttgtt gtcttcgctg acgatcaggc gtttgtagat ctctttgctt tcgtcgaggt
     4381 aaacgtagct acgtgcgcca ggcgtgcgac catgagcatc accaataccg cctacgtcca
     4441 cgcccagcag tttcagcttg gcgctaaggt cagcaccttc aaaggcgttt tcgctaccga
     4501 gaatatggtc aacggcgacc tgcgccattt tgtagccagg tgctaccaga ccaaatatac
     4561 ggttgttcca gcttgcgcat tcaccgatag cgtagatatc cggatcggaa gtttggcagg
     4621 aatcattaat gacgataccc ccacgcggag caacgtccag accacactgg gttgccagct
     4681 tatcgcgcgg acggataccg gtagagaaga cgataaagtc gacttccagt tcgctgccgt
     4741 cggcaaaacg catggtttta cgtgcttcga caccttcctg cacaatctca agggtgtttt
     4801 tgctggtgtg aacgcgcacg cccatacttt cgattttgcg acgcagctgc tcgccaccca
     4861 tctgatcaag ctgttccgcc atcagcatag gggcaaattc gataacgtgg gtttcaatac
     4921 ctaagttttt cagcgcgcca gcggcttcca gacctaacag gccgccacca acaacggcac
     4981 cgcgtttgct gcgacgggcg caggattcaa tggcgttgag gtcttcaatg gtgcgataga
     5041 caaagcagtc ctgagtatca gaacctttga ttggtgggat ccacgggtag gaaccggttg
     5101 ccatgatcag cttgtcataa aaaacggtac gtccggcgct ggagtgaatc accttctcct
     5161 gacggttgat ggtgatagcg cgttcgccga ccagaacttt gatgccgtgt ttctcgtaga
     5221 agccttcgcg caccagcgac agctcttcgg cggtgtggtg agagaagtaa gacgagaggt
     5281 gtacgcggtc ataagcgatg cgcggttctt cacagaaaac ggtaatatca aagttggccg
     5341 catcagattt atcaagaaga tcttcgataa agcgatggcc gaccataccg ttaccgataa
     5401 ttgcgagtct gactttgctc atttttgcct cgatttcttt tctattaccg cctaccttaa
     5461 cgattcagca accccgctta ttgatgtaaa tcaaattcac ctttatatac cccttaaggg
     5521 gtatgttgct gatttgtata aattttctta agtcacggaa ttgtctgaca tttcatgttt
     5581 gtgcaaaaaa tagacaaaat gaacgggttt atagaaatag atgcacgtag cggggttaga
     5641 gaggaaaagg ccggacgacg atagtgccgt ccggcattag caattaatga gaggtcaggg
     5701 tgttatgctg acggtgacgg ctgacgaaac ctaacaggaa gcacatcaca aagacgacag
     5761 cgtacagacc gtttgctgta agcagtgccg cctgcggacc gctatgttca acaatcgggc
     5821 cggtaaccac aaaggtcaac atagtaccga tggttccgca ggtcaggaca aagttaacca
     5881 gttttggcga cggtactttg gtctgctgtg aacccagggt gatgatggtg gtatagatcg
     5941 cgctggagaa gaagcccaga gcgagaattg accacgccat atgtgctggt gttccggtgt
     6001 taaagacgta catcagaatc gcagccagac cagccagtac ggtcagaatg cgttgcaaat
     6061 caaagaagcg aagaataaag ctgaacgccc acatgccgac catgtatgac atccagaagt
     6121 tactcaccag cgtgcccgcg tcgttcaggc tcatgcccag gcctttggca tactcaggca
     6181 cccaggagat aaaacctaac tgaccgagga tgtagcacag cgccgcaaca gagagaaaca
     6241 gtacgccgat cccccacttt tctttctcta ccggagcatc cgtttttggt gcatgtttgc
     6301 ccagtgctgg gaactcacag ccgaaggtca gaataaaaat agcgacatac accagcccga
     6361 tgcaggcata aacccagtac cactcaatgc tgcgcgccag tagaaacgcg gcgatcattg
     6421 ggaaaatcat cccagccata ctgaagaagg agtcagtaaa taacaggcgg gaaccgcgct
     6481 gacgcccttc atacatttgt gttatcagga atgtaccaat cgacatagta atgccgctga
     6541 ccaccccgag aatgaacatc gccgtcgaga acagcgccag gctgtggctg aacatcaaac
     6601 cggcaaccgc cagcaccatc aggagaaagc caaaacgtaa ctgcgttttc aacgggacga
     6661 tttccatcag ccaggcgttg aggaagatag agattaaaat gccggcgttg aggaaggtga
     6721 aggtgttact catactggaa acaggcagat tgaaataatc ggcgatattt cccatcacca
     6781 tcccggtgac aataaccaac gcaccagtca gtgcgtagga gagaaagcta atccatgtga
     6841 gcttgatgcg attgctgtta gtcatgattg gcctgcgttc aaaaataaaa tggcatagcg
     6901 ggatatgccg cgagcgggtg attttaggtg attttgtgat ctgtttaaat gttttattgc
     6961 aatcggttgc taaattgcat tttaagaagt gatcttgatc acggaataaa aagtgatcgt
     7021 caggttacat atatttcaga tacgtaaaat taggtaaagg gatggccttg ttcttgaagg
     7081 ctatttagaa tctcttcact tgcttttttt ctgctctgtt tgttaaggaa atctcatgtt
     7141 caaatcgacc ctggcggcga tggctgctgt tttcgctctt tctgctcttt ctcccgcagc
     7201 aatggcagcg aaaggggccc cgcacgtatt gttgacaacc tcagctggta acatcgaact
     7261 ggagctggat aaacaaaaag cgccagtgtc tgtgcaaaac tttgtcgatt atgtgaacag
     7321 cggtttttat aacaacacta cctttcaccg cgtcattcct ggctttatga ttcagggcgg
     7381 cggtttcacc gagcagatgc agcagaaaaa accaaacccg ccaatcaaaa atgaagccga
     7441 taacggcctg cgcaacacgc gtggcaccat cgcgatggcg cgtaccgctg acaaagatag
     7501 cgccaccagc cagttcttta tcaacgttgc cgataacgcc ttccttgacc atggtcagcg
     7561 tgatttcggt tacgcggtat ttggtaaagt ggtgaaaggc atggacgtgg ccgataagat
     7621 ttcccaggtg ccgactcatg acgttggtcc gtaccagaat gtgccgtcaa aaccggtagt
     7681 tatcctttcc gctaaagtcc tgccgtaatg atttctcgcg cgggcaatct tgcccgcgct
     7741 tctgctctcc cggcgtaacc cggatttgcc gcttatactt gtggcaaatg gacacgttca
     7801 gggaggcatc aagtgaagaa actcaccgat aagcaaaagt cccgtctctg ggagcttcag
     7861 cgtaatcgta atttccaggc cagtcgccgt cttgaaggcg tcgagatgcc tttagtcact
     7921 cttactgccg cagaggcttt agcgcgcctt gtagagctga ggaggcacta tgagcgataa
     7981 attcggcgaa gggcgcgatc cgtatcttta tccaggcctt gatatcatgc gtaaccggct
     8041 gaacatccgc cagcaacaac ggctggaaca ggccgcttac gaaatgacgg cgctgcgtgc
     8101 tgcgaccatt gagcttggtc cgctggtgcg cggtttaccg catttgcgaa ctatccatcg
     8161 ccagttgtat caggatattt tcgactgggc agggcaactg cgtgaagttg atatttatca
     8221 gggcgatacg ccgttctgcc actttgctta tatagaaaaa gagggcaatg ccctgatgca
     8281 ggatctggag gaagaaggtt atctggttgg cctggagaaa acgaagttcg tcgagcggct
     8341 ggcgcattac tattgtgaaa tcaacgtgct gcatcccttc cgggtgggaa gtggtctggc
     8401 acagcggatc ttcttcgagc aactggcgat tcatgccgga tatcaactga gctggcaggg
     8461 tatcgaaaaa gaggcctgga atcaggcaaa tcagagtggg gcaatggggg atctcaccgc
     8521 actgcagatg atatttagca aagtggtaag cgaagccggg gaatctgagt aaaatagcgc
     8581 ggttctttcg taccggagcc gccatgatcc tgcttattga taactacgat tcttttacct
     8641 ggaacctcta ccagtacttt tgtgaactgg gggcggatgt gctggttaag cgcaacgatg
     8701 cgttgacgtt ggcggatatc gacgccctta aaccacaaaa aattgtcatc tcacccggcc
     8761 cctgtacgcc agatgaagcc gggatctccc ttgacgttat tcgccactat gccgggcgct
     8821 tgccgattct tggcgtctgc ctcggtcatc aggcaatggc gcaggcattt ggcggtaaag
     8881 ttgtgcgcgc cgcaaaggtc atgcacggca aaacctcgcc gattacacat aacggtgagg
     8941 gcgtatttcg ggggctggca aatccactta ccgtgacacg ctaccattcg ctggtggtgg
     9001 aacctggctc attaccagcg tgctttgacg tgacggcctg gagcgaaacc cgcgagatta
     9061 tggggattcg ccatcgccag tgggatctgg aaggtgtgca gttccatcca gaaagtattc
     9121 ttagcgaaca aggacatcaa ctgctggcta atttcctgca tctctgattt ccgattgcca
     9181 ttcagtgatt ttttatgcat attttgtgat tataatttca tatttattta tgcgtaacag
     9241 ggtgatcatg agatggcaat tgaacaaaca gcaattacac gcgcgacttt cgatgaagtg
     9301 atcctgccga tttatgctcc ggcagagttt attccggtaa aaggtcaggg cagccgaatc
     9361 tgggatcagc aaggcaagga gtatgtcgat ttcgcgggtg gcattgcagt tacggcgttg
     9421 ggccattgcc atcctgcgct ggtgaacgcg ttaaaaaccc agggcgaaac tctgtggcat
     9481 atcagtaacg ttttcaccaa tgaaccggcg ctgcgtcttg ggcgcaaact gattgaggcg
     9541 acgtttgccg aacgcgtggt gtttatgaac tccggcacgg aagctaacga aaccgccttt
     9601 aaactggcac gccattacgc ctgtgtgcgt catagcccgt tcaaaaccaa aattattgcc
     9661 ttccataacg cttttcatgg tcgctcgctg tttaccgttt cggtgggtgg gcagccaaaa
     9721 tattccgacg gctttgggcc gaaaccggca gacatcatcc acgttccctt taacgatctc
     9781 catgcagtga aagcggtgat ggatgatcac acctgtgcgg tggtggttga gccgatccag
     9841 ggcgagggcg gtgtgacggc atcgacgcca gagtttttgc agggcttgcg cgagctgtgc
     9901 gatcaacatc aggcattatt ggtgtttgat gaagtgcagt gcgggatggg gcggactggc
     9961 gatttgtttg cttacatgta ctacggtgtg acgccggata ttctgacttc tgcgaaagcg
    10021 ttaggcggcg gcttcccgat tagcgccatg ctgaccacgg cggaaattgc ttctgcgttt
    10081 catcctggtt ctcacggttc cacctacggc ggtaatcctc tggcctgtgc agtagcgggc
    10141 gcggcgtttg atatcatcaa tacccctgaa gtgctggaag gcattcaggc gaaacgccag
    10201 cgttttgttg accatttgca gaagatcgat cagcagtacg atgtgtttag cgatattcgc
    10261 ggtatggggc tgttaattgg cgcggagctg aaaccacact acaaaggtcg ggcgcgtgat
    10321 ttcctgtatg cgggtgcaga ggttggcgta atggtgctga atgccggacc ggatgtgatg
    10381 cgtttagcac cgtcgctggt ggtggaagat gcggatatcg atgaagggat gcaacgtttc
    10441 gcccacgcgg tggcgaaggt ggttggggcg taaataagac gcatcaggca aaatatgtcg
    10501 gatgcggcgt gaatgcctta tccgatggtt tagcctgtat tacgccttcg aatcccgcaa
    10561 cttgcgactc agccaaatcc cgtgatgcgg tcgctgacgc catgccatcg acgaaatggt
    10621 gtgcatggtg ttcagatgac caataacccg ctgtaaatgc tgctccagcg tacctgccgc
    10681 gccttcgtgc ggctgcatct ccggcgcatc catgatattg gcatcgccag aactacccgg
    10741 ttcgtcatac tccagtcgct gctgacaacg ctgaatggcg atttcacaag actgtaaata
    10801 ctcttgtgcc agttcaggtg gcaatgcccg gtgttcccgc gccagcgtgg tcatggcatt
    10861 aatatgctca acaataaact ggctgtgcgt tacccacagt ttcatatctg ccagataatg
    10921 gctgttaaac gccggttcct gcatcgcctg attcaatgag ttatacagag tgttatgtgc
    10981 ctgatttacc cgcattcgct gccaggccag tggcgtaggt tgcggatcct cgctaagaat
    11041 caagcgaatc gcttcctgat aggcttctaa agcgtcatgg gcgtttttac gcaataaccc
    11101 gctctgccac tgcggccaca gccagacagt accgccgaaa gcaattaaac aaccaataat
    11161 ggtatcgata agacgcggaa ggatgtattg ctcgccgttc aaccacaata gttgcagggt
    11221 atacactgcg gtaatagtaa aaccgaccgt cgcccagccg tagtttttgc gcaatatcag
    11281 gtagctggcg agggtggtaa tcagcatcaa cgtcagggtg taaccttcgg gaattttaaa
    11341 gtgcagcgcc acgcccgcaa tgattaaccc gaccacggtt cccacggagc gattcacaat
    11401 cctcagacgg gttgcgccat agccattttg tgtcaccagc aataccgtca tcaggatcca
    11461 gtacgacttc ggcagatgca gcgcggtgcc catcaggctg gcaacgctta acatcacact
    11521 gagtcgtccg gcattgcgta gcgccggaga ctttagtgac agataacttt tcagcgcagg
    11581 aagtaatggc attcgccgct gtttatcggc cagtaagtca cgggcataga gcggtttttg
    11641 ggtgcgcagc acgcgggcga tgcggctgaa atgccagtag cagaattgcc caaccggatt
    11701 atccggatgc tggcgggcga ttttttccag tgcgccaatt tgcttttcca tcgtaaaacg
    11761 cgttggcagg cgatggtaaa gaatgtcatc agccagcacg cgcaggcgag cggcgacggt
    11821 ttgcgcattc cagcggataa cttcttccgc atggctacgc tcgaccagct tttgcacctc
    11881 ttccggctga tgcaaactga ccgaaatatg ttcctgtaaa tccagcgcct cctggaaaat
    11941 acgcagcatc cgcttgtagt cagtattatt ttgcgcggaa agcatatgca tttgctgata
    12001 gcactgggta attagatcga ccgctttttg ctggcgcacc agcagcggcg gcagcgcttt
    12061 ttcagggtcg gtgtgctggg taagcaggct gtatttggct tcacaataat ctgccagttc
    12121 acggtacagc agacttagtg actcgcgcag cggttgttcg cgccagatcc agaaccaaaa
    12181 ccagttaaac aatccgtacc agagagtgcc caacgcatag atgagcaacg gttcccagac
    12241 cggcatgtat cccgccagac tgagggtaaa aatggctgcc agcagcgatg cgggaagcaa
    12301 ttttgcgtgc aatggcccca actcagcagt gacgccaagt accagcgtta atccggtcag
    12361 caaaaagggc aggggaacat cttttgccag tagcagctgt gtcagtaagc tacaggtggc
    12421 aaacagcgac gcaccaatga ttaagcgttt gaaaaaacgt ttatgaggcg tatcaaggcc
    12481 cgcaatattg caacaggcag gaacgagaga gaagagcaga ccgaatcgta attcgccaag
    12541 cattaaccca acggccacgg gcaaacatag caccagcgtt tgtcgaagtg catagttgat
    12601 atcggggtga taaatcagtc tgcgccacat cgggggaaac aaaatggcgc gctaccaggt
    12661 aacgcgccac tccgacggga ttaacgagtg ccgtaaacga cgatggtttt accgtgtgcg
    12721 gagatcaggt tctgatcttc aagcatcttc agaatgcgtc ccacggtttc acgagaacag
    12781 ccgacaatct gaccgatttc ctgacgggta attttgattt gcataccgtc cgggtgagtc
    12841 atagcgtctg gttgttttgc cagattcagc agagtctgtg caatgcggcc cgtcacgtcg
    12901 aggaacgcca ggttgcccac tttctctgaa gtgacttgca gacgacgcgc catctgtgca
    12961 gacagacgca tcagaatgtc cgggtttacc tgaatcaatt ggcgaaattt tttgtacgaa
    13021 atttcagcca cttcacaggc ggttttcgca cgtacccatg cgctacgttc ctggccctct
    13081 tcaaacaggc ccagttcgcc aataaaatca ccctgattca gataggagag gatcatttct
    13141 ttaccctctt cgtctttgat cagcactgcc acagagcctt taacgatgta gtacagcgtt
    13201 tccgcttttt caccctggtg aataagcgtg ctcttggatg ggtacttatg aatgtggcaa
    13261 tgagacaaga accattcgag agtcgggtct gtttgcggtt tgccaagcac catgcgcggt
    13321 tatcctctgt tataagcttt ctccagagcc agataacgcc gctgtctctg gattgccgaa
    13381 atatgcttcc cgctacctgg gaaggggcta tcaactgtac tgcacggtaa tgtgacgtcc
    13441 tttgcataca tgcagtacat caatgtatta ctgtagcatc ctgactgttt tagcatagct
    13501 ttcgctttgt gtctcctggt gtctcgcttc agcatgaccc aggtcgcctt ccgttgcgcg
    13561 atttggttag tacgcgt
//
LOCUS       DAJTSL010000103        13490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTSL010000103 DAJTSL010000000
VERSION     DAJTSL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.103"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..186
                     /locus_tag="OO433_002754"
     CDS             <1..186
                     /locus_tag="OO433_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6051169.1"
                     /translation="CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELL
                     NRRLSESVPCMPHKKAG"
     gene            207..908
                     /gene="mlrA"
                     /locus_tag="OO433_002755"
     CDS             207..908
                     /gene="mlrA"
                     /locus_tag="OO433_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR6051170.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDDGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARRKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            968..1075
                     /locus_tag="OO433_002756"
     CDS             968..1075
                     /locus_tag="OO433_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6051171.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1056..1787)
                     /gene="yehW"
                     /locus_tag="OO433_002757"
     CDS             complement(1056..1787)
                     /gene="yehW"
                     /locus_tag="OO433_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6051172.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFVVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1792..2718)
                     /gene="yehX"
                     /locus_tag="OO433_002758"
     CDS             complement(1792..2718)
                     /gene="yehX"
                     /locus_tag="OO433_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6051173.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVCREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(2711..3868)
                     /gene="yehY"
                     /locus_tag="OO433_002759"
     CDS             complement(2711..3868)
                     /gene="yehY"
                     /locus_tag="OO433_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6051174.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3875..4792)
                     /gene="osmF"
                     /locus_tag="OO433_002760"
     CDS             complement(3875..4792)
                     /gene="osmF"
                     /locus_tag="OO433_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6051175.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQISGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5044..7341)
                     /gene="bglX"
                     /locus_tag="OO433_002761"
     CDS             complement(5044..7341)
                     /gene="bglX"
                     /locus_tag="OO433_002761"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6051176.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPFTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGSKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDMVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7537..9252
                     /gene="dld"
                     /locus_tag="OO433_002762"
     CDS             7537..9252
                     /gene="dld"
                     /locus_tag="OO433_002762"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6051177.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPSSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALQRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(9291..10223)
                     /gene="pbpG"
                     /locus_tag="OO433_002763"
     CDS             complement(9291..10223)
                     /gene="pbpG"
                     /locus_tag="OO433_002763"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6051178.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(10397..10984)
                     /locus_tag="OO433_002764"
     CDS             complement(10397..10984)
                     /locus_tag="OO433_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6051179.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11154..11732
                     /locus_tag="OO433_002765"
     CDS             11154..11732
                     /locus_tag="OO433_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6051180.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(11862..12623)
                     /locus_tag="OO433_002766"
     CDS             complement(11862..12623)
                     /locus_tag="OO433_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6051181.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(12676..>13490)
                     /locus_tag="OO433_002767"
     CDS             complement(12676..>13490)
                     /locus_tag="OO433_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HCR6051182.1"
                     /translation="EKEQNTIIATDRQLYQTGITSSVEGVETDINASKTRQQLNDVAG
                     KMKIIEARLSALTNNQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAAHWYVES
                     SLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNA
                     NLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDTLRVVGLA
                     QARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGGGYKR"
BASE COUNT         3236 a         3770 c         3405 g         3079 t
ORIGIN      
        1 tgctggtcac ttgccgcagg agctaatcct agttttatcg caagccagat gggccacaca
       61 aacgcacaaa tggtattcaa tgtttacgga gcatggatga aagacaacaa tcacgaacag
      121 atagaacttc ttaacagaag actatctgaa agtgtcccat gcatgcccca taagaaagcg
      181 gggtaaaata aaaacttgca aaatcaattg gtttaccttt aatccctgtc acgttacgcg
      241 cgtggcagag gcgttacggg ttgctgaaac cgcaacggac agacgacggt catcgactgt
      301 tcaacgatgc cgatattgac cggatccgcg agatcaaacg ctggatcgac aacggcgtgc
      361 aggtcagcaa agttaaaatg ctgctcagta atgaaaatgt tgatgtgcag aacggctggc
      421 gcgatcagca agaaacatta ctgacctacc tgcaaagcgg caatctgcat agcctgcgaa
      481 cgtggatcaa agagcgcggt caggattacc ccgcccagac actcaccaca catctgttta
      541 ttcctctgcg ccgacggctt cagtgccaac aaccgactct ccaggcgctg ctggcgatcc
      601 tcgacggcgt actgatcaac tacatcgcca tttgtctggc ttcggcacgt agaaaacagg
      661 gtaaagatgc gctggtggtt ggctggaata ttcaggatac cacccgtctg tggctggagg
      721 gctggattgc cagtcaacaa ggatggcgca ttgatgtcct cgcccactcg ctcaatcaac
      781 tacgccctga actattcgaa ggccgtacat tgctggtgtg gtgcggtgaa aatcgaacct
      841 ccgcccaaca gcagcaactc accagttggc aagaacaagg ccatgatatt ttcccactcg
      901 gcatttaatg attcgttaac aaatgcgctt tactgtacaa tcctttcgtt aacataagga
      961 gtgcaatatg cgcatagcta aaattggggt catcgccctg ttcctgttta tggcgttagg
     1021 cggaattggt ggcgtcatgc tcgcaggtta tacctttatt ttgcgtgctg gctaagcgcc
     1081 tgcaccagcc tttcaaacag gcggtctgca atgatcgccg ccagtgccac cagtaacgcc
     1141 ccctggatca catacgcggt attaaatccg ctaagcccga tgatgatggg cgtacccagc
     1201 gtgctggccc ctaccgttga ggcgatcgtc gccgtaccaa tgttgataat caccgaagtt
     1261 cgcacgcccg ccagaatcac cggagccgcc agcggtagct cgaccttacg cagtcgctga
     1321 ccacgactca ttcccatacc tttcgcaact tctgtcacgc tggcatcaat cgctcccagc
     1381 ccggcaagtg tcgcctgcag gacgggcagc acaccgtaaa ggatcaaggc gataatcgct
     1441 ggttgcagac caaagccgat caccggaacg gcaatcgcca gcactgcgac aggcggaaaa
     1501 gtctgtccaa cggcggcaat agtttccacc agtgggcgaa attccgcgcc ccacgggcga
     1561 gtgacagcaa ttccggcacc agtgccaatg atcaccacaa acaaactcga aattcccacc
     1621 agccagaaat gagccagtgc cagagctgca aaactttctt gctgataaac gggtcgtggc
     1681 agttgtggga acaaggcagc aaacagcggc tggctgtaag gcagccagaa aatcagtgcc
     1741 acaaacaaag caatgagcca gaacagcgga tcgcgcaaca tcttcatacg cttacgcctc
     1801 ctccagcaga tcctgaaaat gcagcgtgcc gcaaggctgg ccctgcgtgt tcaccaccgg
     1861 cagcacctcg catccccgcg cgacaaacag ggagagcgca tcgcgtagcg tcatctcttc
     1921 tgccagtgcc tcaccttctg cccgttcttc gcgacacacg taatccgcca cactacgtaa
     1981 cgaaagcagg cgcacaccca gttcgctgcg cccaaaaaac tggcggacaa aatcgtttgc
     2041 cggacgagtc agcatcgtca gcggattgcc ctgctgcacc acttcaccgt gatccatcaa
     2101 taccagatgt tctgccagcc gtagcgcctc atcaatatca tgagtgacca gcacaatggt
     2161 acgccccagc aaacggtgaa tgcgcgtcat ctcttgttgc aacgcgccgc gcgttaccgg
     2221 gtccagtgcg ccaaaaggtt catccatcag taagacttgc ggatcggcag ccagtgcgcg
     2281 cgccactccc acacgttgct gctgaccacc ggaaagctga tgcggataac gctcacgtaa
     2341 atttgactcc agccccagta gcgccattaa ttcgtcgata cgatcgtcaa tccgcgcccg
     2401 cgaccatttt tgtaattgcg gcacggtagc gatgttttgc gccacgctcc agtgggggaa
     2461 caggccaata gattgaatag catagcccat ccggcggcgc aactccagta ctggcagcga
     2521 gcgaatttct tctccggcaa agcggatcac tccgctgtca tgctccacca ggcggttaat
     2581 cattttcagg gtggtggatt tgccggagcc agatgtgcca atcagcaccg aaaaactccc
     2641 ttcctgaaaa ttgagattga gatcgttaac ggctttttgt gcgccgaaca gtttgctgac
     2701 atggctaaat tcaatcatta cgtttcacct tcagcagtgc gataagcaaa tcgaacagcg
     2761 cgtcggtaag caccgccaga acaattaccg ggatcacccc cagcaacact aaatcaatgg
     2821 cgctgcttag cagcccctgg aaaaccagcg caccaaaacc gcctgcgccg attaacgccg
     2881 caatcaccgc catacctaca gtttgcacca tcaccacccg caggctgcgc agaaataccg
     2941 gtaacgccag tggtaactga acatgcagga atcgctgcgc cccgctcatg cccatcgctc
     3001 tggcactctc cagcacatcg cgcgggatct ggttcaaacc gactaccacg ccgcgcacca
     3061 gcggcagcaa ggcatagagc accagcgcaa tcagtgcggg tgtcattccg gttcctgcta
     3121 tgccgagctt ccccagccac ggaaaggccg tcaccagcgc ggcaagcggc gcaatcaaca
     3181 ggccaaagag cgccaccgaa ggcacggtct gaatgacatt gagcagagaa aaaattgccc
     3241 cctgccgagc agtggaaaag tagcaccaga tgcccaacgg cacaccaatc actaacgcag
     3301 gcagcaccgc accaaacagc aacgtcagat gttgtgccag cgcgtcgtca aacacatcct
     3361 gacggttggc gtattctttc attagtgaga gatcgttaag cgtgccggag tacagcaacc
     3421 acagcggaat aatggcaatc tgcatatgca acaaccagcg ccacagcgga tgcgtggaga
     3481 ttcggcggat ggcatcgcta caggccagca atgccagcgc cgcagccagc cagaaaccac
     3541 tgccgaggct ggtacgcgcc agcgcactgc cattttgcgc cagttgtgtc gccgcctttc
     3601 ccgcgcccca caccagcaat acgaagacga attgcgccag aatgagtgca caaatgctgc
     3661 cttttttacc gggaataaaa caggccgtca gccaggcgca accaacgccc accagcatcc
     3721 agatcgtttg cggccacagc tgccagagat gacgcccctc acccgaaact aaacgattag
     3781 gcgcgtaact gataaacggc agcgccgctg cgattgccgt cagcaacagc agcagcgcca
     3841 gaacaggatt aatacggaaa taagtcacgg gaaattactt cgtccaccct ttttgtttca
     3901 ggtagtcggc agccactttt ttggcatcca gtccttccac agcaatgctg gcattcagtt
     3961 gctgcaatgt ttttgcatcg aggctggcga agactggctg tagccactgt gccatttgcg
     4021 gatactcctt caacaccgac tcacgcacca ctggtgcagg cgcgtagata ggttgcacac
     4081 cttgcggatc gcttaaggtt tgcagcccca gcgccgctac cgggccgtca gtgccgtaag
     4141 ccattgcagc attaacgcca gagatttgct gggcagcggc tttgatcgtc accgccgtgt
     4201 cgccaccagc cagtgacagc aactgatcct gaccgagctt aaagccatag gctttttcaa
     4261 acgcgggtaa cgcatcggcg cgttcgataa actctgccga ggctgccagt ttgaaggtgc
     4321 cgccctcttt cagataacga ctcaggtcgg caagcgaagt gagtttattt ttctctgcca
     4381 catcctgacg cacggcgata gtccaggtgt tatttgcagg cgcgggcgtc agccagatta
     4441 acttgttgtg ctccgaatcg agctttttga ctttctcgta accttgctgc gcgtttttcc
     4501 acgctgcatc gttttcatct ttaaagaaga aagcgccatt gccggtatat tccggataga
     4561 tatccagttc accggaagta atcgccccgc gcaccacagg agtcgttccg agttgcactt
     4621 tattcaccgt cggtacgccg tggctttcca gcacctgcaa gatgatgttg ccgagtagcg
     4681 cgccttcggt atcgattttt gaaccgactt taacggggga agccgcctgt agcggcaggc
     4741 tcacggctgc caacataacc agtgaacctg cccagacctt tgagagtggc atgatgcttt
     4801 cctcattctt ttactgttgt tttcagcgaa ttaagagaaa agcatagttg ataatgacag
     4861 agttagcctg agcggaagga ttcagttgca gaatcagata aatacttaaa gggcatattt
     4921 tgcccggcgt ttcttgccgg atgcggcgta aacgccttat ccggcctaca aaggtcgaaa
     4981 attcagcaag ttaagatatg aacatcaggc aaagggcgca aacgtcgcgc ccttactaaa
     5041 gcattacagc aactcaaact cgcctttctt aacgcgtgcg gaatcagtgc cgataaagac
     5101 attgaacttg ccaggctcgg cgtcatattt catctgttga ttccagaact tcagcgcctc
     5161 gatatcaatc gggaagctga cagtctgagt ttcgcccggt ttcagggtga ttttctcaaa
     5221 gcctttcagc tgcttcactg gacggctcat ggaagccgtc acatcctgca agtacatctg
     5281 tactaccgtg gccccttcgc gtttaccggt gttcgtcacc tgcacgctgg ccgtcacttt
     5341 gccgtcacgc ttcatggtcg gcgcagaaag tttcacatca gagacggtga aagtggtata
     5401 gctcagacca tagccgaacg gataaagcgc cccgttagct tcatcaaaat aacgcgaagt
     5461 gtatttgttc ggcttgtcgg cattatacgg acgaccggtg ttcagatggc tgtagtacac
     5521 cgggatctgc ccgacagaac gcgggaagga catcggcagc ttgccggacg ggttgtaatc
     5581 gccaaacaac acatcggcaa ttgcattacc gccttcagtc cccgcaaacc aggtttccag
     5641 aatcgcatca gcctgctgat cttctttcac cagcgccagc ggacgcccgt tcatcagcac
     5701 cagcaccagc ggtttaccgg tggctttcag cgccgcaatc aagtcacgtt ggctttgcgg
     5761 aatagtgata tcggtacggc tggaggcctc gtgcgccatc ccctgtgctt caccgaccac
     5821 agccaccacc atatcagatt gtttcgccgt ctgcaccgct tcatcaatca tctcttgcgg
     5881 cgagcgcgga tcgactttga ccgcttcttc atactgattc aggaaatcga taatgccttt
     5941 gtcactggta acgttcgccc ctttggcata cagcacttta ccgttttcac cgacggcatt
     6001 tttaatcccg gtcagtacgg tcacggattg atcggcaaca ccggctgcgg accagctgcc
     6061 catcacgtcg cgtttactgt ccgccagtgg cccaaccacc gcaatggtgg ccgatttttt
     6121 cagcggtaac gtttcgagac ggtttttcag caacaccagg ctttcgcgcg ccacttcacg
     6181 tgcttcttta cggtgcaggc ggctttcggc attggtatcc accgggtcag actctttcga
     6241 ccccaaatgg ctgtatgggt cgttaaacaa tcccatatca tatttaacgt tcagtacatg
     6301 gcgggcagca tcgtccagct cttccatcgt cactttgccg gatttgatca acccaggcag
     6361 atacttcgag taatactcgt cgctcatact catgttgatt ccggatttca gcgccacgcg
     6421 caccgcatct tccgggtctg ccgccgtgcc atgtttaatc agctctttga ttgcaccgtg
     6481 atcggaaacg gtgatgcctt taaagcccca ctggtcgcgc agaacatctt tcagcagcca
     6541 ggaatcggag gtggctggcg tgccgttcag cgagttcagc gccaccatca ccgcgccgct
     6601 gcctgcgtcc agccccgctt tgtacggcgg catataatca ttaaacaggc gctgcggact
     6661 catatcgacg gtgttgtact ctttaccgcc ttctaccgcg ccgtatgcgg caaagtgttt
     6721 gacgctggtc atcaccgagt agcgatctgc cgggctttta ccctgcatcg cttccaccat
     6781 ggttttaccc attgttgagg tgagatacgt atcttcgcca aaaccttcgg aagcacgtcc
     6841 ccagcgcgga tcgcgcgaga catcgaccat cggtgcccag gtcatattca ggccatcatc
     6901 tgccgcttca taagcagaga cacgtccgac cgttttcact gcatcgaggt taaaagacga
     6961 ggccagaccg aggctaatcg ggaacaccgt gcgctgaccg tgcagcacgt cgtaagcaaa
     7021 gaaaagagga attttcaggc ggcttaattc catcacctga tcctgcatgg cgcggatatc
     7081 ctgacgggtt acggtgttga aaatcgcccc aacctgaccg tctttgatca tctcgcggat
     7141 cgcctctttc gggttatccg ggccgacgct gattaagcgc agctgaccaa ttttctcatc
     7201 aactgtcatt ttcttaagca attcggtgac gaacgcatcc cgcgcttcgg gcgtgaatgg
     7261 atggttgccg aataaatcat ccgccagtgc tggctgcagg gccagactca ccgcgattcc
     7321 tactgaacat agccatttca tatggatttt tcttcctctg ttgcccgacg aggcagcgaa
     7381 aatggtgcaa aaaccgtagt ttgccataag catgatggag agagaaaaag aatgctcagt
     7441 ttattgtctg aattttcaaa atattcactc gctgaattgt catacaaggc gctattctag
     7501 tttgtgatat tttttcgcca ccacaaggag tggaaaatgt cttccatgac aacaactgat
     7561 aataaagcct ttttgaatga acttgcccgt ctggtcggtc attcacacct gctcaccgat
     7621 cccgcaaaaa cggcccgcta tcgcaagggc ttccgttctg gtcagggcga cgcgctggct
     7681 gtcgttttcc ctagctcact actggaattg tggcgggtgc tgaaagcctg tgtcaccgcc
     7741 gacaaaatta ttctgatgca ggctgccaat acaggcctga ccgaaggatc gacgccaaac
     7801 ggtaacgact atgatcgcga tatcgtgatc atcagcaccc tgcgtctcga caagctgcac
     7861 gttcttggca agggcgaaca ggtgctggcc tatccgggca ccacgctcta ttcgctggaa
     7921 aaagccctca aaccgctggg acgcgaaccg cactcagtga ttggatcatc gtgtataggc
     7981 gcatcggtca tcggcggtat ttgtaacaac tccggcggct cactggtgca acgtggcccg
     8041 gcgtataccg aaatgtcgtt attcgcgcgt ataaatgaag acggcaaact gacgctggtg
     8101 aaccatctgg ggattgatct gggcgaaacg ccggagcaga tccttagcaa gctggatgat
     8161 gatcgcatca aagatgacga tgtgcgtcac gatggtcgtc acgcccacga ttatgactat
     8221 gtccaccgcg ttcgtgatat tgaagccgac acgcccgcac gttataacgc cgatcctgat
     8281 cggttatttg aatcttctgg ttgcgccggg aagctggcgg tctttgcagt acgtctagat
     8341 accttcgaag cggaaaaaaa tcagcaggtg ttttatatcg gcaccaacca gccggaagtg
     8401 ctgaccgaaa tccgccgtca tattctggct aacttcgaaa atctgccggt tgccggggaa
     8461 tatatgcacc gggatatcta cgatattgcg gaaaaatacg gcaaagacac cttcctgatg
     8521 attgataagt taggcaccga caagatgccg ttcttcttta atctcaaggg acgcaccgat
     8581 gcgatgctgg agaaagtgaa attcttccgt ccgcatttta ctgaccgtgc gatgcaaaaa
     8641 ttcggtcacc tgttccccag ccatttaccg ccgcgcatga aaaactggcg cgataaatac
     8701 gagcatcatc tgctgttaaa aatggcgggc gatggcgtcg gtgaagccaa atcgtggctg
     8761 gtagattatt tcaaacaggc cgaaggcgat ttctttgtct gtacgccgga ggaaggcagc
     8821 aaagcgtttt tacaccgttt cgccgctgcg ggcgcagcaa ttcgttatca ggcggtgcat
     8881 tccgatgaag tcgaagacat tctggcgttg gatatcgctc tgcagcgtaa cgacaccgag
     8941 tggtatgagc atttaccgcc ggagatcgac agccagctgg tgcacaagct ctattacggc
     9001 cattttatgt gctatgtctt ccatcaggat tacatagtga aaaaaggcgt ggatgtgcat
     9061 gcgttgaaag aacagatgct ggaactgcta cagcagcgtg gcgcgcaata ccctgccgag
     9121 cataacgtcg gtcatttgta taaagcaccg gagacgttgc agaagtttta tcgcgagaac
     9181 gatccgacca acagtatgaa tccggggatc ggtaaaacca gtaagcggaa aaactggcag
     9241 gaagtggagt aaaaattacg gatggcatag tatcgccatc cgtcattcac ttaatcgttc
     9301 tgtgccgtct gccccgccgc cgccatttgg gcggcttttt gttttttata gctcaacgct
     9361 gctgctggaa caggcatcac tttaccggtt tcaatccagg tacgcaggcg gctggcatcg
     9421 gcaaaatggg tatatttgcc aaacgcatcc atcactacca gcgccaccgg tttattattg
     9481 ataaccgtac gcatcaccag acaatggccc gccgcattgg taaaaccggt tttggttaac
     9541 tgaatattcc agttatcgcg atacaccaga tggttagtat tgcggaacgg cagcgtatac
     9601 gtcgggttag agaaggttgc catatcttcc cgagtagtac ttaactgccc gatcaacgga
     9661 tattgtttgc tggcaatgag cagtttggtt aagtcacggg cagttgaaac gttatgcacc
     9721 gacaatccgg taggttcaac aaagcgcgtg ttgttcattc cgagcgattt cgctttcgca
     9781 ttcattgcct taataaaggc tttgtaacca ccgggataat ggtgtgcaag gcttgccgcc
     9841 gcgcggtttt ctgaagacat cagcgccagc aacagcatat ctttacggct gatttcgcta
     9901 ttcagtcgta cgcgcgaata gacccctttc atctccggcg tctggctgat atccactttt
     9961 agtttttcat ccagcggcag tcgtgcatcc agcacaacca tcgcggtcat taatttgctg
    10021 atagacgcaa tcggacgcac cagatccggg tggttcgagt agatcacttt gttggtattc
    10081 agatcaacaa tcatcgcgct accggaggca atttcaggtt gtgaagcggt gatagcggct
    10141 gccgttttcg caacggcctg cggtgccaaa ggcacagcca gcatcagggc caggctaaat
    10201 aaagaaactc gaaatttcgg catgatgagc attcagatag tggttcacgc gcacaggttg
    10261 cgcaccgccg gagtaaggat ttactgaggc tagcgacgcc atcataacga gcaaaaagtg
    10321 cgatcgtcaa aggagaatcg tgaggaaatg ctgcattgct gacatttacg ccagcaatgc
    10381 aacgtcaaag aactttctag aacaaacgat aaccgtagcc ccatagtata acggttaggg
    10441 cgagcagcac ttccagtacc agcacgccaa tcgccagcgt cgaactagag aagctaaggc
    10501 cttcctcttt gttgatattc aggaagctcg gaataccaag gtaaagcagg tagccggtgt
    10561 aaaacagcgc caccgtgccg accagcgcac acaaccagac cagtggataa agcgccacca
    10621 aaccgcttaa aaacagcgga gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagtg
    10681 acggacgctg cggataatta cgcgccatcc accagatgac ccgccccatt accgcaaccc
    10741 cagccagcat aacgccataa aacaggacag ccagcgccag tccggtaaac caggataact
    10801 tcaggatagt gccatcgcca aaattccagc caatctgtgt agtgccaatg aaggcgcaaa
    10861 tcaccggaat cgccgccatc agcaaaacgt ggtgggtgta atggtgagaa atcgtttcgt
    10921 tttcgcgatt aatcacctgc atttcacgat cgggatggga aaacagtccc cagacatggc
    10981 tcataccgcc cccttgttgt gagttcatga acctgacagt tcaagtataa gtcagcttgt
    11041 gattattttt tgttcgctca tgtaatttgc accgtctgat gtacgtcctt tttcagcgtg
    11101 aatgatttcc agggtgtatg cttacaggca accaaaggga gacagactgg cctatggatc
    11161 tcaatacact catctcacaa tatggttatg ccgcgctggt gatcggtagc ctggcggaag
    11221 gtgaaaccgt gactttgctg ggaggcgttg cggcacatca ggggctatta aagttcccgc
    11281 tggtggtact ttctgtggcg cttggcggca tgattggcga ccaggtgctt tatctgtgcg
    11341 ggcggcggtt tggcggcaag ctgttacgcc gtttctcgaa acatcaggat aaaattgagc
    11401 gggcgcagaa acttatccaa cgccatccgt atctgtttgt cattggtacg cgctttatgt
    11461 atggctttcg ggtgattggc ccgacgctga ttggtgccag ccagctgccg ccgaaaatct
    11521 ttctgccgct gaatattctc ggcgcatttg cctgggcgtt gatttttacc actattggtt
    11581 acgctggtgg tcaggtgatt gcgccgtggt tgcacaatct cgaccagcat ttgaagcact
    11641 gggtctggtt gattctggtc gtggttctgg tggtgggcgt gcgctggtgg ctgaaacggc
    11701 gcgggaagaa aaagccggat catcaggcgt aaaaccattg ccctgggtaa ggcgttcgcg
    11761 ccgcatccga catccggtgc accatgcctg atgcgacgct tgtgcgtctt atcatgccta
    11821 ccaaaatttt acccattgaa ataaccacgc acctcactgc gctattctgg gttgaactgc
    11881 ggattcgcca acataaagcc gccatccact atcaacgact gcccggtggt gtaatttgcg
    11941 ccctctgagc acagccacgc taccaggctg gcaatctcat gcgttgcgcc aaaacgccgc
    12001 agaggaatcg aaggctccgc gtcgggcttc acgtcgctgt catccatgcc attcattggc
    12061 gtggcgatcg ccccaggcgc gactgcgttc accaaaatct tatgcctgac cagctccagc
    12121 gccatcgctt tggttaaccc accgagcgca tgtttagcgg ctgtgtaggc gctggcatcc
    12181 ggcagcggcg tatgttcatg taccgatgta atgttgatga tgcgaccgcc ctgcccttgt
    12241 ttcaccatct ggcgggccgc aatttgtgag cataaaaatg caccatcgac atcaacggta
    12301 aaaatcttgc gccactcatc gaaagccata tcaagaaacg gcgctttggt cattgcgccc
    12361 gcattattca ccagcacatc aatacgcccc agccgttgaa tgagtttctc cagcgcctgc
    12421 gcaccttctg gcagtttacc gagatccagc tgcacgatct ccgcacgtac gccgtggcta
    12481 actacctcac gcgcggtatc ttttgcccct tcttcatctg agtgccaggt aatgccaata
    12541 tcaaaccctt gctgcgccag taataacgcg cactctttgc cgatccccga atcggaggcg
    12601 gtaataatcg caacctgtgc catcgagttc tccacttaac gctgaataaa cgttaagtat
    12661 agaaggcgca tatcatcagc gtttgtaacc cccgcccaac gcaccagtga gttgaatgga
    12721 ggcatccagc cactgccctt gcaataacag gccattggcc cgctcacgca gcgcggggat
    12781 tctggcttcg ctgacgcggg aaccagcaat gatgcccgcg ttaaagcgcg cctgcgcaag
    12841 acctaccaca cgcaaggtat cgcgctcaat ttgcgcctga tgctggtttt tctccgcaag
    12901 tgtctgaacc tgactggctg cccgcgccac gtcattcacc gcttcaacca ccgctttgtt
    12961 gtagctggcg atagacaagt tgctttcggc ttttgcgata tcgagattgg cgttaagacg
    13021 accactatcg aaaatgggta gcgtcaggcc tgccgtaacg cccatttgct gcgcggaatg
    13081 acggaacaga tcgcttaagt gcaacgcatc ctgttgcagg aaggccatca ggttgatgtc
    13141 aggataaaat gccgcttttg ccgcatcaat ggtgcttagc gatgactcaa cgtaccagtg
    13201 cgccgcctgc aaatctgccc gccgggccag taaggagtac cccagttcat caggaagctg
    13261 gcttgccact ttcggcaacg cgaccggttt aagcttcaat gactttgtct ggttatttgt
    13321 aagtgcgctt aaccgtgcct caataatttt cattttcccc gcgacatcgt tgagctgctg
    13381 ccgggttttg ctggcattaa tatcggtttc cacaccttca actgaagaag taatcccggt
    13441 ctgatatagc tggcgatcgg tcgcgataat ggtgttctgc tctttttcta
//
LOCUS       DAJTSL010000104        13479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTSL010000104 DAJTSL010000000
VERSION     DAJTSL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.104"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(102..3323)
                     /gene="carB"
                     /locus_tag="OO433_002768"
     CDS             complement(102..3323)
                     /gene="carB"
                     /locus_tag="OO433_002768"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6051183.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3341..4489)
                     /gene="carA"
                     /locus_tag="OO433_002769"
     CDS             complement(3341..4489)
                     /gene="carA"
                     /locus_tag="OO433_002769"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6051184.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(4944..5765)
                     /gene="dapB"
                     /locus_tag="OO433_002770"
     CDS             complement(4944..5765)
                     /gene="dapB"
                     /locus_tag="OO433_002770"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6051185.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSRESHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(5932..6846)
                     /gene="rihC"
                     /locus_tag="OO433_002771"
     CDS             complement(5932..6846)
                     /gene="rihC"
                     /locus_tag="OO433_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6051186.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(6912..7862)
                     /gene="ispH"
                     /locus_tag="OO433_002772"
                     /gene_synonym="lytB"
     CDS             complement(6912..7862)
                     /gene="ispH"
                     /locus_tag="OO433_002772"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6051187.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(7864..8313)
                     /gene="fkpB"
                     /locus_tag="OO433_002773"
     CDS             complement(7864..8313)
                     /gene="fkpB"
                     /locus_tag="OO433_002773"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6051188.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(8382..8876)
                     /gene="lspA"
                     /locus_tag="OO433_002774"
     CDS             complement(8382..8876)
                     /gene="lspA"
                     /locus_tag="OO433_002774"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6051189.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            complement(8876..11692)
                     /gene="ileS"
                     /locus_tag="OO433_002775"
     CDS             complement(8876..11692)
                     /gene="ileS"
                     /locus_tag="OO433_002775"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6051190.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYHDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(11735..12676)
                     /gene="ribF"
                     /locus_tag="OO433_002776"
     CDS             complement(11735..12676)
                     /gene="ribF"
                     /locus_tag="OO433_002776"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6051191.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYVVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(12684..12902)
                     /locus_tag="OO433_002777"
     CDS             complement(12684..12902)
                     /locus_tag="OO433_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6051192.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            13005..13268
                     /gene="rpsT"
                     /locus_tag="OO433_002778"
     CDS             13005..13268
                     /gene="rpsT"
                     /locus_tag="OO433_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6051193.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3148 a         3805 c         3398 g         3128 t
ORIGIN      
        1 aaaagattta cttaatgggc aaaaaaaacg gtcctcatca gagaaccgta ttacttattc
       61 gatcaaatta gcggatgaaa aatatccgcc atgacacgct attatttgat ctgcgcgtgc
      121 atttcctgca ccgaaattac tttttcggtc gcgtcggcat tcagcgccat cgcggtagcg
      181 aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc
      241 acgcgggagt cttcaatcgc gcgacggcct gaagtggtgt tgatgatgta ggtatattcg
      301 ccattcttga tgcggtcctg aatgtgcgga cggccttcat gcaccttgtt caccagacgc
      361 ggattaatgc ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag
      421 ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg
      481 gaaagcaacg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggca
      541 aacgcttcag cgaaggtgcg gcccacgccc atcacttccc cggtggagcg catttctggc
      601 cctaacagcg ggtcaacacc cgggaattta ttgaacggca gcaccacttc tttcaccgag
      661 tagtacggcg ggataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc
      721 acacgcgccg ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacggta
      781 cgcgccgcac gcgggttaac ttcaatcagg tagacttcgt tgtttttcac cgcaaactgc
      841 acgttcatca gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc
      901 acatcctgaa tttcctgact taaggtgtag gctggcagag aacatgcgga gtcaccggag
      961 tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg
     1021 cagatggcat ccacgtcaac ttctaccgcg tcatcaagga agtgatccag caacactggc
     1081 gcatcgttag acacgctgac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag
     1141 acgatttcca tcgcccgacc gccgagaacg taagacggac gtaccaccag cgggtagcca
     1201 atctctttcg ccttctctac cgccatttca atagcggtaa cggtggcgtt cgccggttgt
     1261 ttcagtttca gacgctcaac cgcatgctgg aagcgttcac ggtcttctgc acggtcgata
     1321 gcatccgggc tggtgccgat aaccggtacg ccagcagctt ccagcgcgcg cgccagtttc
     1381 agcggggtct gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt
     1441 tccagcacat cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg
     1501 gtggagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcgcgcagc
     1561 gccagcgagg cgtgtacaca gcagtagtcg aattcgatac cctgaccgat acggttcggg
     1621 ccgccgccga gaaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct
     1681 tcataagtgg agtacatgta agcggtgtcg gtggcgaact ctgccgcaca ggtatccacg
     1741 cgcttataaa ccgggtgcag gtcatactgg tcgcgcagct tgcggatttc cgcttcgcgc
     1801 acgcccgcca gttttgccag acgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg
     1861 aattcagcgt tcaggccagt gatgcccact tccgctactt tctcttccag acgcaccagt
     1921 tcttcaatct gtaccaggaa ccagcggtca atattggtca ggttgaacac gccgtccaca
     1981 gacaggcccg cacggaaggc atcggcgatg taccagatac gctctgcgcc agcatctttc
     2041 agttcgcgac ggattttggt taacgcttcc gggtcatcga ggctcacttt cgggtcgaat
     2101 ccagtcgcac cgacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga
     2161 ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcacca
     2221 gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg
     2281 aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcgtcgag ggtgtaaccc
     2341 accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc
     2401 gaagaacggg acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc
     2461 accgcaaact ggacattgga accaccggtt tcaacaccga tttcacgcag caccgccatc
     2521 gaggcgttac gcatgatttg atattctttg tcggtcagcg tttgggctgg cgcgacagtg
     2581 atggagtcac cggtatggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg
     2641 cagttgtcgt ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac
     2701 tcatcaatca gcagctcttt ggttggggag agatccagac cgcgggcgca aatttcttca
     2761 aactcttcgc ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga
     2821 ataatgcacg ggaagcccac gtcagcggca accgccagcg cttcttccat cgtgtgtgcg
     2881 ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg
     2941 tcttctgctt tatcaatcgc atcggcggtc gcaccaatca tagtcacgcc gaactcttcc
     3001 aacacgccct gacgctccag ctccagagcg cagttcagcg ccgtctgacc gcccatcgtt
     3061 ggcagcaccg cgtccgggcg ctctttttca ataatcttgc gtactacttc ccagtgaatc
     3121 ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag
     3181 ttcaccagaa tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag
     3241 tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt
     3301 tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt
     3361 actgctcaat taactcgata aagtggtcga acaacggcgc tgcgtcgtgt ggcccagggc
     3421 tggcttccgg gtgaccctgg aagctgaacg ccggtttatc agtgcgatga atgccctgta
     3481 acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat
     3541 ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa
     3601 ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca
     3661 gcgccagcag ctgatgaccg aggcagatgc cgaataccgg aatatcggtt tcgaggaatt
     3721 tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga
     3781 agatgccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg
     3841 tcaggcgaca gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat
     3901 aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca
     3961 acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat
     4021 ccatgccatt cagacctggg aacgcgcggg ctttttctaa cgccaacgcc gcatccgggt
     4081 tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct
     4141 tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt
     4201 cttcggtatt acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg
     4261 catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat
     4321 aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg
     4381 tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac
     4441 cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa acaccctcca
     4501 gagaatattc actcacttta tttgcatatt aattcacaat gatgacgtaa atcaatgcaa
     4561 atctgcttac cgacgaattt ctggcaaacg gcggcattct ggagatatgg gcgctaaaag
     4621 tcaacttaat ggcgggattt tttatattat tttgtggctc atgcatgttt tacaggcatt
     4681 aagcgacaaa aagatcatat aacctgttct ggaaaacgct tgcacagcaa gaagattgca
     4741 tgaaataaaa gcgagcaggg aaaagtggac caaatggtca aattttacat taaaacaaca
     4801 aaaacagaag acaaaacatg taatttttgg cacttataga caatttaaaa acataacact
     4861 aaaaataaaa gggccaaaat taaagccctt tataatcaat aaattaaatg tgttattttt
     4921 gcaccataac aaatattttg tggttataaa ctattgagat caagcacatc tcgcatatca
     4981 aaaagaccac tttccttacc actcaaccac aaagccgatc ttaccgcgcc gttagcaaat
     5041 gtcatgcggc tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc
     5101 gcggtatgtt cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtgccaggc
     5161 acacgttcac cggtgtggct ttcacgactg tagaccgcgc aatctttcag atctttatca
     5221 agggcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta
     5281 tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct
     5341 ttctccagca gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc
     5401 gcaatatcgg cggcagcgtc acgaatcgcc tgtttaccgg cttcgtcaaa ccccgtagtg
     5461 ccgatcacca tccctttgcc atgctggcga caaaaagcga gatggttcag cgtaccttcc
     5521 ggacgggtaa aatctataaa cacatcaaaa tcatctttta ccgcatcgag gctgctttgc
     5581 acggtaacgc ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa
     5641 gatccttcgc gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag cgccgcctga
     5701 atcaactggc ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca
     5761 tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg
     5821 ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc
     5881 agtgaaaggc atgagtcgat gaatgactgc atgccgataa catgtgacag gttacgacgc
     5941 cagagccagc acctcagcca cccactgctg gaagcctttc acatccagat ccaatgccac
     6001 ctttacattg gctggtttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt
     6061 aaattcgccc tgagtttcca ctgccacaaa acacggtttg agagtgaaca attccgggcg
     6121 caccagccag gcaatagcgc aaagatcgtg catacgcagt ccactttgca tactgccgct
     6181 acggtagtgg ctaaacaggg cgtggagcat ttttccggtg cgattcaact ccggcaaagt
     6241 agcgagatag tcaggggtta atattgcctg attggtgaca tccagaccgc tcatgacgat
     6301 ttcgatgccg ctacggaaga cacaggcagc cgcttctgga tcggcagcga tattaaactc
     6361 ggcgtttggt gtgcagttgc cgcgaccagc agaaccgccc atgatcacca gacgacgaat
     6421 atacggttta cattccgggc attgtgaaag taacagcgca atattggtta acgggccgat
     6481 ggccaccagg gtaacgggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc
     6541 tggtatcccg agcggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc
     6601 gccgtgcaca gatgccgcat cacgcggtgc gcgtaccagc ggcacagcgg ctccctgggc
     6661 gagcggaatc tccgcattcc agaaatgcag cagttgcagg gcgttgcggg tggttttctc
     6721 aaccgagaca ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gtgcaaaaat
     6781 cgcggcggca atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa
     6841 atgcatgttt tctccataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg
     6901 ctgctaatga cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa
     6961 acaatgtttt cttcacggcc ttccagcgga atggcttcac caccgcccag ctgctgcaaa
     7021 cgtgccacca cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgacg
     7081 catttaacct ctttcaccca ctcttcctgg atgtctttcg catcgtcaat caaaaacgcg
     7141 cgtttgccca tacgctgggc cagctccgcc agacggttgg agttggagga gtttttcgaa
     7201 ccgaccacca acacaacttc cgcctgttct gccagggcgc gtaccgcttc ctgacggtta
     7261 gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc
     7321 agcgcgtcga tcacatcaga cgtgtcatcc accgacagcg tggtctgggt cataaaggag
     7381 agcttctctt cgtttttgac cgtcagtttc cacacatcgt ccggcgattc gaccagatac
     7441 attccccctt ccgggttact gtactggccc attgtccctt ccacttccgg gtgcccggcg
     7501 tgaccgatga gaatagattc ttcgccacgg cgactggcgc gggcgacttc catatgcact
     7561 ttggtcacca gcggacaggt cgcgtcaaat accgtcaaat cgcgactttt tgcttcgtta
     7621 cgtaccgcct gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc cggtacttcg
     7681 ctaatctgct caataaagat agccccacgc tcacgcaagc tatcgaccac atagcggtta
     7741 tgtaccactt cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca
     7801 atgctgatag cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc
     7861 atgttacgcc tccaatgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg
     7921 cccggccaga ggatggttga aatcaacggt aatggagtcg ccgttaattt cgcggatcac
     7981 gccaggcatc tcactgccat ccattgcggt aaaaagcatg attgcgccaa tttctggctc
     8041 gcctgcatcc ataaattcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc
     8101 aaacgccgca tctggctcca acgagaaggt ggttttatcg cccactttca gccccaacag
     8161 gtgttgctcc agcccttcag aaagagaagc atcacccagg cggaacagcg ccggtttacc
     8221 gttgttgcgg gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag
     8281 gacggcgcta ttgctctgta cagattcaga catgcaggtt tgctcttata tttgaggatt
     8341 tttgccggat gcggcgtaaa cgccttatcc ggcagggttt attattgttt tttcgctcta
     8401 gaaggcaaaa aaccttccag cacaatcagt gccgcaccga cacagatggc agtatcggca
     8461 aggttgaagg tggcgaagtg ccagtcgccg acgtagaagt cgatcatatc gacaacgaag
     8521 ccgtgccaca ggcggtcgaa caggttgccc agcgcgccgc caataatcag cgcgtaagcg
     8581 atattgttta gcttctgcgt ggccttcgag cgatacatca tcactgccag gatcacgcta
     8641 ataccaatcg caataccggc aaagaaccaa cgctgccagc cgccgctatc ggcaaggaaa
     8701 ctaaacgccg cgccatagtt acgcgcataa tgcagattaa gcgacgggaa cagcgggacc
     8761 gtatccccca gagcaaagtt ctggaggatc aggtatttgc tgcccagatc gataatcagc
     8821 acgactacca ccagccacag ccagcgtagc cctgttgaac agatcgattg actcatcagg
     8881 caaacttacg tttttcaccg tcaccggcga cgttgctgac acagcggccg cagatttctg
     8941 cgtgttccgc caccttgccg acatcctggg tgtagtgcca gcagcgtggg cacttctcac
     9001 cttcggcttt actcaacgcg actttcagcc ctttgagtac ttcgctctgc tgagcatcag
     9061 caggtgcgtc gtgatagtct gcaacggtag cgccggaggt caacaggaca aatcgtaatt
     9121 catcgcccag cgcggtcagt ttcgccgcca gttccggttc tgcatacaag gttactgccg
     9181 cttccagcga gccacccact ttcttgtcgg cacgcgcttg ctcaatgact ttgttcactt
     9241 cgccacgcac tttcaacagc tcgtcccaga acgcatcgtt catcgcttcg ctgtctgcca
     9301 ggccaaacag gccttcgtac cactcaccgg tgaagacgta tttttcacgt tcgcccggca
     9361 ggtagcccca cacttcatca gcggtgaagg agaggattgg tgccatccag cgcaccagcg
     9421 cttctgcgat gtgatacagc gcagtctggc agctacgacg cgccacactg tccgctttgg
     9481 cggtgtactg acggtctttg atgatgtcga ggtagaagga acccatctca acggagcaga
     9541 agcgcatcag acgctgtacc acttcgtgga aatcgtatgc ttcgtacgcc ttgaggatgt
     9601 cttcctgtgc cgctttcgca caacctacgg cccagcgatc cagtaccacc atctcttccg
     9661 gtttcaccat atcttttgct ggatcaaaac cgttcaggtt tgccagcagg aagcgcgcgg
     9721 tgttacggat acgacgatag ctatcggcag cacgtttcag gatctcgtca gaaacggcca
     9781 tttcaccggt gtagtcggtt gatgccaccc acagacgcag aatatccgcg cccagtttgt
     9841 tcatcacatc ctgcggcgaa acggtattgc cgatggattt agacatcttg cggccctgac
     9901 catccacggt aaagccgtgg gtcagtacct gacgatacgg cgctttgcct ttcatcgcgg
     9961 tggagatcat tagggaagac atgaaccaac cacggtgctg gtcagaacct tccagataca
    10021 tgtccgctgc gtgaccggca aattccggac gcacgtcaac aacagaagag tgggtagatc
    10081 cggagtcaaa ccatacatcc aatgtgtccg gcactttcac gtactgatca gcttcatcgc
    10141 cgaggatctc tttcgcatca agatcccacc acgcctggat gccgtcaact tcaacgcgtt
    10201 ttgccacttc ttccattagt tcgagagtac gcggatgcag ctcttccgtg tctttgtgca
    10261 cgaacagtga catcggtacg ccccaggtgc gctgacggga gatacaccag tcaggacggt
    10321 tagcaaccat cgactcgata cgcgcctggc cccagtccgg gatccactgc acgcctttga
    10381 tctctttcag tgactgcgca cgcagacctt tctgatccat gctgacgaac cactgcggcg
    10441 tcgcgcggaa gatgatcggc gttttgtgac gccagcagca cggatagctg tgctgcattt
    10501 tctcaacgtg cagcagcgcg cctttttcct gcagcagcgc aacgacgatg tcgttcgctt
    10561 tgaagacgtt cacgccatcc agcgtcggat aagtgcccgg cagataagtg ccgtccgggc
    10621 caaccgggtt agcggtttcc aggccgtatt tctgaccgat cacatagtcg tccgggccgt
    10681 ggccaggcgc ggtgtgaacg gcaccggtac cggcatccag ggtaacgtga tcgccgagga
    10741 ttgccggaac gtcgaagccc ataaacggat gggtaaagcg cagcaactca agctccgcac
    10801 cttttaccgt gccgagaatg gtgtaatcgg tcacgccgat acgctgcatt acgctttcaa
    10861 ccagatcttt cgccagaatc acggcctgac cgtcgatctg caccagcgca tagtcgaaat
    10921 caggtgcaat agagattgcg cggttcgcag gcagagtcca cggcgtagtg gtccagatca
    10981 ccagcgagat tgggccgtta acgttgctta cggcaaattt tgctttcagt gcatcctgat
    11041 cgaccgcctg aaaagcaacg tcgatggacg gagaagtttt gtcgtaatac tcaacttccg
    11101 cttccgccag cgcagaacgg cagtcaacgc accagtgtac cggcttcgcg cctttgtgca
    11161 ggtgaccgtt gccgatgatt ttgcccagcg cgcggatgat gttggcttca gttttgaagt
    11221 ccatggtcag gtacgggtgc gaccagtcgc ccagcacgcc cagacggata aagtctttgc
    11281 gttgaccgtc aacctgggtc gccgcgtatt cgcggcactt ggcgcggaac tcggcggcgg
    11341 tgaatttctc acccggctta ccgtattctt gctctacttt cagctcgatc ggcagaccgt
    11401 ggcagtccca gccaggcaca tacggcgagt cataaccgga aagccctttg gacttcacga
    11461 taatgtcttt cagaatcttg ttaaccgagt gaccaatatg aatgctgcca ttcgcataag
    11521 gagggccatc atgcagaatg aaggtttttt tgcctttttt agccgcacgg atgatgccgt
    11581 acagatcatc atcagtccaa cgcgccagca ttcccggttc gcgcttggcg agatcgccac
    11641 gcatcgggaa ccctgtttcc ggcaaattca gggttgattt atagtcactc atcagattct
    11701 cggttccgtg tttcggtttg attacataac aggcttaagc cggttttgtt agcccaaaaa
    11761 attcgcgggc ggttaattca tcacgcgcaa tctgcgcttt cagttcgtcc agcgacgcaa
    11821 atcgctgctc attgcgtatt tttttacgca gcactacttg tatatggcga ccgtaaaggt
    11881 ccattgcaac atctaacaaa tgcacttcca gctgctggcg aataccggca accgttgggc
    11941 gtgttccgat gtttgccacg ccgggtaacg gcttttcacc gaggcccagc acttctacca
    12001 cataaacccc tttcaccggg gaaacctgac ggcgtagcgg tacattcgcc gtcgggaaac
    12061 ctatagtgcg ccctaattca tcaccgtgga ctacacgccc ggagatggca aacgggtgcc
    12121 ccagtaaact ttctgccaga gccagattgt catccgccag cgcctgacgc acagcggtgc
    12181 tgctaatacg cacgccacct tcgcaaaaag tttgcgtact agtgatatcg aagccgtatt
    12241 ccatgccagc tttctgtaat aacaagaaat cgccttcacg accagcgcca aagcggaaat
    12301 catcccctac ggcaagaaat tttacgcgca aatgcttcac cagaagatcg ctgatgaaat
    12361 tttgcgcggt taacgccgcg aaacgcctgt cgaaacgcac gcacagcacg taatcaacgc
    12421 cacactctgc aaggtaacgc agtttttccc gcagccgggt cagtcttgcc ggggctttat
    12481 cggtagcaaa cagttccagt ggttgaggtt caaaaagcat caccatcacc ggtaagttgc
    12541 gcttgcgccc ttcttcctgc aagccctgta acagcgcgcg atgaccgcga tgcacgccgt
    12601 cgaaattacc aatagtcagc acacaccctt cttgcggggc ctggctgaga ttatgtatgc
    12661 cgcgtatcag cttcatgtct ggctcaaaac agtgaaaatc gtccgagtat accttgtaca
    12721 gcggtcaagg ttaaccggcg attgagtacc gaaatcagaa agaagtggct tttcattgcc
    12781 atggcgcaaa tcacgggaag aaagtgaccg cctgctgcaa tttttatcgc ggaaaagctg
    12841 tattcacacc ccgcaagctg gtagaatcct gcgccatcac tacgtaacga gtgccggcac
    12901 attaacggcg cttatttgca caaatccatt gacaaaagaa ggctaaaagg gcatattcct
    12961 cggcctttga attgtccata tagaacacat ttgggagttg gaccttggct aatatcaaat
    13021 cagctaagaa gcgcgccatt cagtctgaaa aggctcgtaa gcacaacgca agccgtcgct
    13081 ctatgatgcg tactttcatc aagaaagtat acgcagctat cgaagctggc gacaaagctg
    13141 ctgcacagaa agcatttaac gaaatgcaac cgatcgtgga ccgtcaggct gctaaaggtc
    13201 tgatccacaa aaacaaagct gcacgtcata aggctaacct gactgcacag atcaacaaac
    13261 tggcttaatc gccaatttgc tgaagctttg tgaaaaagcc cgcgcaagcg ggttttttta
    13321 tgcctgctgc ttttatgatg cgtgagagaa cacactaccg gttgccagat agtcaaaaga
    13381 tgctggcttt tgcagacacg ccgttacact ttctgaactc ttcgtggctt aagaatcgta
    13441 gaaccatcgg ctgatacatt ctgaacaaga atatctact
//
LOCUS       DAJTSL010000105        13391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTSL010000105 DAJTSL010000000
VERSION     DAJTSL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.105"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..393
                     /locus_tag="OO433_002779"
     CDS             <1..393
                     /locus_tag="OO433_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCR6051194.1"
                     /translation="NVKSGGVGARYFGIGADTQYQLDQIAVNLRVVNVSTGEILSSVN
                     TSKTILSYEVQAGVFRFIDYQRLLEGEVGYTSNEPVMLCLMSAIETGVIFLINDGIDR
                     GLWDLQNKAERQNDILVKYRHMSVPPES"
     gene            complement(457..948)
                     /locus_tag="OO433_002780"
     CDS             complement(457..948)
                     /locus_tag="OO433_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6051195.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1050..1604)
                     /gene="ycdY"
                     /locus_tag="OO433_002781"
     CDS             complement(1050..1604)
                     /gene="ycdY"
                     /locus_tag="OO433_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6051196.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWMEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(1628..2365)
                     /gene="ycdX"
                     /locus_tag="OO433_002782"
     CDS             complement(1628..2365)
                     /gene="ycdX"
                     /locus_tag="OO433_002782"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6051197.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(2420..3358)
                     /gene="ghrA"
                     /locus_tag="OO433_002783"
     CDS             complement(2420..3358)
                     /gene="ghrA"
                     /locus_tag="OO433_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR6051198.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            3592..3679
                     /locus_tag="OO433_002784"
     tRNA            3592..3679
                     /locus_tag="OO433_002784"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3626..3628,aa:Ser,seq:gga)
     gene            complement(3829..4671)
                     /locus_tag="OO433_002785"
     CDS             complement(3829..4671)
                     /locus_tag="OO433_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR6051199.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKTIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(4756..4953)
                     /locus_tag="OO433_002786"
     CDS             complement(4756..4953)
                     /locus_tag="OO433_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051200.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(4965..5456)
                     /locus_tag="OO433_002787"
     CDS             complement(4965..5456)
                     /locus_tag="OO433_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051201.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(5453..5827)
                     /locus_tag="OO433_002788"
     CDS             complement(5453..5827)
                     /locus_tag="OO433_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6051202.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(5874..6248)
                     /locus_tag="OO433_002789"
     CDS             complement(5874..6248)
                     /locus_tag="OO433_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR6051203.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(6411..6632)
                     /locus_tag="OO433_002790"
     CDS             complement(6411..6632)
                     /locus_tag="OO433_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6051204.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6695..7171)
                     /locus_tag="OO433_002791"
     CDS             complement(6695..7171)
                     /locus_tag="OO433_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6051205.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(7187..7660)
                     /locus_tag="OO433_002792"
     CDS             complement(7187..7660)
                     /locus_tag="OO433_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6051206.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(8002..8820)
                     /locus_tag="OO433_002793"
     CDS             complement(8002..8820)
                     /locus_tag="OO433_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6051207.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(8975..9133)
                     /locus_tag="OO433_002794"
     CDS             complement(8975..9133)
                     /locus_tag="OO433_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR6051208.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(9204..12050)
                     /gene="ag43"
                     /locus_tag="OO433_002795"
     CDS             complement(9204..12050)
                     /gene="ag43"
                     /locus_tag="OO433_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR6051209.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            complement(12422..13294)
                     /locus_tag="OO433_002796"
     CDS             complement(12422..13294)
                     /locus_tag="OO433_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR6051210.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT         3117 a         3611 c         3481 g         3182 t
ORIGIN      
        1 aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga cacgcaatac
       61 cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg tgagatcctc
      121 tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg ggttttccgc
      181 tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa cgaacccgtt
      241 atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa tgatggtatc
      301 gaccgtggtc tgtgggattt gcaaaataaa gcagaacggc agaatgacat tctggtgaaa
      361 taccgccata tgtcggttcc accggaatcc tgacagataa aaaagccgga attatcatcc
      421 ggcattttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt tgatttcctg
      481 ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt ttttcattgc
      541 gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt caccctgccc
      601 ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca gcagctgttt
      661 ggcctgaata cacatcagaa acgccacaat accggttatg acataaccga gaatttccag
      721 atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc cactaagcag
      781 ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaggcaa agtaggccgt
      841 acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc atccccagat
      901 accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc cgcacatgat
      961 agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa caaattatct
     1021 gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc cagctcgtcc
     1081 cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa aggcgtggtt
     1141 gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata ctcactgaac
     1201 agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccatcca ggaagccgca
     1261 agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtggcatccc tcgttcggaa
     1321 agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg atatggcggc
     1381 acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg cgccatatca
     1441 cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt cgcagccagt
     1501 ttcccctcac gaatcagggt aaacagcggc accagtaaag gatcttgcgg ttggcggtaa
     1561 tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat atttccatta
     1621 ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat tcaaggaagt
     1681 tcagtaagcg gcgcggagaa acattcaaaa tgcgctctgg cggaaaatct accgcgtcga
     1741 ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa tccgagccta
     1801 atgccaccca acctccagca tcacgtaccg ctgcggctac cgcacgacag ttgtcttcac
     1861 tgcccttacg tgagtgtaaa aatgaagaat tattgatttc cagcgccacc tgatgtttcg
     1921 cggctgcttc agcaacggct ttcacatcta tttcatattt gggatttccg ggatggctta
     1981 ttatatgcac attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg gtcgctttgt
     2041 catgtggtgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc gagtcaaaca
     2101 ttttaccgct gcaatcaatt tcaccatcaa catttttaat gttagcttcg atgccgcgca
     2161 ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag tgccagtgat
     2221 gcggagcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta atgccctttt
     2281 gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg gcaacggtat
     2341 gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg ccaaggatag
     2401 caggaatcct gatgctttat tagtagccgc gtgcgcggtc gacttgcccg cagaccctct
     2461 cccctttttc gagctgggca atggtgcgag aaatgtactc cacagcttca gcgggacggg
     2521 taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc ggactttcag
     2581 gcggtaaggg ttcacgatta aaaacatcca acattgcgcc tttaactttg ccgctatcca
     2641 gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg aggagatacg
     2701 cgccatccgg taatttttcg agtaattgtt gattaataat gccgacggtt tcaggggtat
     2761 tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt tcttcccgtc
     2821 cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa cgcagcggaa
     2881 agcgccaggt ttgcagactc tgagcaactt tactgcccag tacgcctgcg cccaaaatgc
     2941 cgatggtaaa atcttcccaa tgatattcag gcagcggttg ccaatgcgaa ctattttgct
     3001 ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca gcatattcct
     3061 gcatttgctc gcccatacca gtatcttcca ggcgaaaaag tggaacagaa gggttcagca
     3121 tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg agtgcgaaca
     3181 ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact aaagcataat
     3241 cagcagagtc attatctccg cttttccatg ctctgactct tgcctgagga atagctttgc
     3301 gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg atatccatac
     3361 tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat ctacaaaggc
     3421 caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt attaaaacca
     3481 cacattgatt gaagtttgaa taaacgcgcg attttttcaa aaagtttgtt gacctcaggt
     3541 catgatttcc ctaaattagc gcccgttcca gcaagacagg aacaacaatt tggtgaggtg
     3601 tccgagcggt tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat cgggggttcg
     3661 aatccccccc tcaccgccag attataagcg catgttattg tttaacatgc gcttttttat
     3721 ttttccttcc agacagtatg ccatcgggta tgccatctga aaccggttta tatgccccgc
     3781 gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct atactgccag
     3841 catatcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt ctggttttct
     3901 gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct catcttcata
     3961 gctctcttta ccctgaacag actgaatgtg atcatccaga cgaagggtga tgtttccccg
     4021 gttgtcgggg attgttttac cgctgaacag gtgcaacatt ctttccagat cggcgagtcg
     4081 gtcagcctgt tgtccggtga taagatgaaa tccccaccgg tcccatctca ccagattgtt
     4141 gacgataatt ttactgccaa atttacaggg gcaattggtt ttgtaattcc agttcagccc
     4201 tctgaagacg ttgatcaccc ctcgctcaaa cacctcatcc ttattctggt gcagttgttc
     4261 aaatgtgctc agaatgttcg cttcgctgat ttccggaaaa ttatcgtact ccagtgaccg
     4321 gtaccatgta tcacgggcct gcgtatccat taatgacagc atcccggaac gttgcatcag
     4381 gtcacgccag atttcgcgat caagattccg ggtaatggct ttcattgctg tttcgggttt
     4441 ctccatcagc cagcagccgc agcgaaaatc ctgtttcatg gcccagtccg gggcagtttt
     4501 acctccgatg ctttccgtca gcaccgagat ttcagccagt tgtcggataa gtgtgtcgat
     4561 ttgcaacagt gtactctttc gtacagccag aatgtggcga atgctggttg agcagataac
     4621 gtccgtatgc ccgttgacca cttccggacg ggagattgtg atatctgaca taatcgatat
     4681 tcctttttgc agataaatga aaacgccagc cctgtgcagg ctggcgtcag aggaatgaag
     4741 tctggtgagt gagcttcacc ggttctggtg cagaagctgc agatgacgca gggtacggac
     4801 atcctcccgg gcctgttcga tacagggcaa cagtgctgct gaatccgttt tatcctcatc
     4861 gttgtcaaag ataattcccg atccgcagtc gatattgtcc tgcagccacg caatcagaat
     4921 atccagcgct gtttccgtgg ttaatgattt catgttgttc ctggttacca gtcgaaagtg
     4981 ggtaaacccg gcaggcattc agcgctggca tccagatgaa tgagactgac gccataacgc
     5041 cggatgagtg tgacgaccag acgacggaac gtttttgaca gccccaggcg tttaagccgc
     5101 agaaccgggt atgaccacgc atcggtacgt aacagatatc cgctacctgt aaaatgaatc
     5161 cattctgatt caccgaagtc actggtctgg tgtgacagcg agtacaacca ggcgttgtcc
     5221 gtttccgtga tatgtgcggt actgcagcgt atgccggtaa gggtagcaaa tggtggcgtg
     5281 cattctgctg aggtctggtc agattcatca acaacacgaa gggagtagcc gttatcgcag
     5341 accatgttaa tcagttcagc gagctcaata ccatcgacgt caacgacaat gcgccccatg
     5401 ttcagtgcct gtacgttaac gctgtcggct tccagtgtca gggccagttt catcatttcg
     5461 cctccggata cttacccagg gtaatgttat ttaccgttct gtaattgtcg cgggtcatca
     5521 ggccggtcgc ccggcgagcc cggaggatat caatactgtt tattaactga gagcggggac
     5581 aggtgctgaa tcccggctgg tcggtacgca ccagcgcgta tttttccacg agaaaattaa
     5641 ctgcatcaca cagtgaaatg cctgcctcaa tatgctgctc aatcacacgt tcatcgacaa
     5701 acggtgtgtc attcagtgtg aggccataat gctggtccag cagtcggatg agcagtgtct
     5761 gccagatggt gacgggagac gggcagcgag atacctcccg tacatgtgtg tcggataatg
     5821 ttttcatctt aagttctctg taactggtag tgctaaaggt gagagcttcg gggttatgag
     5881 gtggttgcgg gtgctggtgt cggataaaca gccagataaa cgtaaccaca ggagccgagg
     5941 gtgtcggctt cgcaggtcag cccttttgca tacagcgtga cggtatgctg atatcgggga
     6001 ttcagttcac cgctggtgag catgagttcc agttgtttca tcagcagcgg aaaggcctgg
     6061 tccagatggt atgcatctgc gtcgctgaag cggcctctga taccggcgcg gtcggcaagg
     6121 taatacaggc ggttaccctc ttgcaccaga cgagcgccaa aacagggcct cactgtgcag
     6181 ggaagccccc accaggggcg gtcgtgattg tcatcgggat gagttatccc ggagagtttg
     6241 tctgacacga taaaatcctc tctgaaaagt ggttaagaat ggtttaacga tgacaggtca
     6301 gccggcgcgc tgaccggttt cggtgaagat gtatgcattg atgagaatgg cctcatccac
     6361 agcggcgtct gaggtcatct ggtcgtactc gtttcccggg tgacggaaca tcagttcagg
     6421 gtgacactca tcagccggac atacgggcca aaactgtcct tacggcgttc tgcaaacacg
     6481 gcaagaacgc cgggaatatc ctgcacttca cgaccagtat acgcttcagc actgccgtgc
     6541 cagcgatact taccggtaca gaacggaaaa agacgggatg caggatgctg ttggtgaata
     6601 cgcatggctt caccacgggt gatgattctc atgatgtgat acctctgaag acagaagata
     6661 aaagtgaaaa caggtgtgat gtggttgtga cgggttaaag cagaccatgt tcggcaaagg
     6721 agaaaacctg gttgccaccg actatcagat ggtccggcac ccggatatcc accaggccca
     6781 gtgcctgtac cagacgttcg gtgataagcc ggtctgcctt actgggtgtg acttcaccgg
     6841 acgggtgatt gtgcgccagc accacggcag cggcattgtg gtacagggcg cgtttaatca
     6901 cttcccgggg atgaacttcc gtacggttga tggtgccggt gaagagggtt tcaccggcaa
     6961 tcagctgatt ctggttgttc agatacagca cccggaactc ttcccgctcc agtcccgcca
     7021 tgttcagaat cagccattcc cgtgccgcac gggtggaggt gaaggccacg ccgggttcat
     7081 gaagatggcg gtccagggtt tgcagggccc gcagaatgag actgcgctcg ccgggcgtca
     7141 tgtttccggg cagaaaggaa agctgctgca ttgtccctgc ctccattcag tcgatgatgc
     7201 gcataatggc gctgcattcc ggatgctgca gggcgtaatc ccgcagccgg taataatgga
     7261 ccgtcatggc ataacactcc gtacgacagg catgatgact gtacgtcatc agacaggcag
     7321 caatgccggc ggcttccggg ctcatttcag cgcggttacc gttcatggca ttgaacagta
     7381 cccatgtttc gtcatcatcg tcatccggtt cgggtgccat aaatgccccg ccgttgttca
     7441 gggtgtacag attccagata ccaccgcagt agtcttcaca cagacggtcc atccagccga
     7501 agacacgggg ttccagggtg acccactgtg gaatgaggcc aaaatgctgc ggccagaagc
     7561 tgatgcgctg ttcatcaggg acgggagtgg caaccagttg ttgcaggcca gtctccggtg
     7621 cacaggccga agcggtggtg ttctgagaca atgttttcat gtatgaaatc cttgtaatgt
     7681 gaataaatga gagggagtaa aggtcgggag ctggcaacat cacgttcatc actgaccgtg
     7741 atgacccggt aatatcaggg atgaatgata ttcggctgaa attccggaca gccgtacgcg
     7801 taaccgttcc ggtcacagac ggatggccag atgccattgc ctgtatactc agtctgcaca
     7861 gactgatttc tccgggacgg taaagcagac gggtacacca ggcgcacagg tcatcatcag
     7921 aaatggaacg acaccccggc agcgcctccg gggcgtcggg tgattctggt gcctgaatca
     7981 cgccttcagg gacatgctgc atcagcgcag gctctccagc agcgtttctg ccattaccca
     8041 caatgcgcgg ttgagcttaa tatcggtgtc gatgttgtga atggcacggg tgtggatacg
     8101 ttttcctttt gcactgcggc cggaaatccc gcctttcagc atattctcct ggatggtctg
     8161 atatgcgctc cacaggtctt taccgtaatc ctccctgcgt cgtggtgtca gaatgtcagc
     8221 ggtggtgact ggctgatgtt cgtcaccata acggtaagtc agtgccgcct gtgccagcgc
     8281 ctggcgtgcc ggtggcggca ggaccagcga ctgcatggca tcacgctttt cctcaatccg
     8341 gtcaaaaacc cccaccactt cgtaagcccc ttcgataact ttctccacca catttccccg
     8401 gtgtggaaca cgcacttccc ccagagactg accgcagacg cagccattct ggcagacgaa
     8461 tctgaagtaa cccggcagca tctggtagct ggaggtaccg tcatgagagt tgagcagaat
     8521 aatttcaggg acatgttctc cgtttatctc tccggcccgc cgcagacgca gcatgtgttt
     8581 tgtgtatccc cggcggcccg ggtcgcgcac gcgggtctgg caggcgaaga atggctgaaa
     8641 accttcctgc tgcaggcttt ccagtacggt gatggtggga atgtacgcat atcgtttact
     8701 gcgggaagta tgccggtctt caccgaaaat actgggaaca tagtgcatca gttcttcgtg
     8761 tgtcagcgga cggtcacggc gtatctggtt cgcataacca aaacgactgg ctagtcgcat
     8821 aatttgctcc ttatcggtgg ttacgattta ctggtgtaat aaatgaaaaa gctgcgtctc
     8881 ccggagaaga cgcggcctga cagatgaaat gaatgacgtt tattgtctga gaagccctta
     8941 actggcgagc tgagtattaa gctgtgttcc ggcatcacca gcgcaactga ccttcagcat
     9001 tacggataac cagccgggaa tatgttccct ggtcatcttc agtaaacaca ttgcggtaag
     9061 ctgttttgac ggcaacagcc tgttcgcgtg agaaagggcc ttcgggcaat aaacgtgatg
     9121 tacaacccgg catatctgtt gctccctgaa agtaaaaagc cccggtcatg atgaccgggg
     9181 cctgaaggag agtgacctga ttatcaaaaa gtcacattca gcgtggcctg accgttataa
     9241 ccttcagcgc tgctgccgct gacgctgtgg gcataaccgg cctgaacgcc cagggtgata
     9301 ttttcccgga cacgggcttc cagtccggcc tgcaggtcca gtgacgtgcc attccgggac
     9361 ggtgagaacg tcatgttact gccggcggcg gctgtcccca tgctcatgtc accccgggag
     9421 ctgaaagtgc ggataacaga aggctgtacc caccagttca ccggcagttc actcacacgg
     9481 tgttttgtac tgtcgcgcag agtgtcacgg gatgaggtgc cttcaccaaa gctcatatcg
     9541 ttgtggctgc ccagacggaa accggcacgc acatgttgtg cactgccatg cccgaacttc
     9601 acataaccgg cgttatcctg gccgtcatcc agggtaagtc cctgccaggt atagtgcagt
     9661 tgtggctcca gcatcaggtt gtcagtgata ctgaagggca gaccggtttc cagtgagccc
     9721 agccagcccc agccccgggc acggaagtcg ttattgtccg atgacgcttt catgctgtgg
     9781 cgggttccct gtgccacaat gtcagcccac aggccggagg acgtgtgtgt cagattcagg
     9841 tatccgccca ggctgccggc atcatcccgg accgtgccgg cgcgggaacc gtcatcatcc
     9901 ttaacatcaa cggaagaatg gcctgcggca ccatacaccc ctgtcgtcag agacataccg
     9961 gcaacctctg ttctcagcag gtcaccctcc agacggacga agccatagct gccgctgctt
    10021 tccggcgtgg ctccacgggc aataccgccg ttgttatcgt gaccgagatg accgccctga
    10081 atgctgagac ggacgctgtt attttcaccg tttacaccgg tctgatggct gcgggagcct
    10141 gccagaatcc ggtcatagtc cattgcctgt gtcagcatgg atgtatacag ggggacttca
    10201 gcacgataag cattttcact gcgcaggtac cagtcttcat cgctgtcacg gttcagggtg
    10261 tagttaaagg cgccggcctg aagcgggcga ctcagggcaa acgcaccttc ttctgtggtg
    10321 gcgccattct gtgcatccac aacccggata ccctgtccgg aggttgccac cccgaggtta
    10381 ctgtttccga catttgtaaa cgcaagccag gttttgccgg ttgcctgacc accattaatc
    10441 accagctggt cagaggtatt gctgccatca aggcgaacac gcatattgat ggtgccaccc
    10501 tgaccggtga ggttactggt ggtcagttta tggaacgtta ccggggagtc gccttttgca
    10561 acagcacggc tcagcgcggc atccggtgtc gtctgattat caaagcgaat ctcccctgcg
    10621 ttaacaatat cgccggtact gaccacatcg ccattcagta ccatgctggc acctttactg
    10681 agctgtgttc tgccactcag acttcccccg gcagccagcg tcagtgttcc acgatgtccg
    10741 acagtggtgt tgctggccag tcctccggca ttaaccgtaa agctgccgcc attttccagc
    10801 agcagtccgc tggcctgacc ggatgtgcca tcaatactga acttaccgct ggcattggtc
    10861 ccctcaacag tggcaccact gtctgcaatc agggcaccac cggatgtcat ggtgacacct
    10921 gttgccttac caccggcaga cactgccagg gttccgccgt catccacccg tgttttctgc
    10981 gctgaatggc cttccagtac atccaggcga ccgccggact ccagaacaac tccgtcagcc
    11041 ttaccgtttt ccaccgtgaa attgcccaga cggttgctgc caagaaccgt tgccgcagta
    11101 ctggtaacca gtgctccacc ctgtttcagg gtgacatgtg tggcggtacc accggcgttc
    11161 acctgcagtt tgcctttctg gttaacggtg gtgaaatccg ccagaccacc ttccttgata
    11221 atctgccagc cgtcactgtt tacaacagtg ccagacgccg taccgccgtt gtgcacatac
    11281 tggtaaccgc cattcagtgt ggtatccagc gcatgcccgt gtaccgtctg gtcgccgccg
    11341 gcataaacca cagtggtatt tgctgttcct tcagcagcca caatctgacg accattttca
    11401 ttgatagtgg tacgtacggc atttccgcga acaaactgcc cggtatcacc attttctgca
    11461 tctggtcccc cttccgcgcc ggtattcaca accgtgtcgg ttgccattgc gccggatttg
    11521 acggtctgcc agcccttctc attaatgacg gtacccgttg caatcccgcc ttcatgtacc
    11581 cactgctcac cgccgttcag agtggtattc actgcctgcc cctgaaggct ctgtccgcct
    11641 ccggcactga taaccgtgtc tgaaacgctt cctccggcat tcactctctg aagaccacca
    11701 ccggtgacag tagtgttgtt ggcgataccg ccattttgta tccattgccc gccggtattg
    11761 gcctcgttat ccggcccata ctccagaccg ctactgatgg tcattccgtt ggccgtaccg
    11821 agaacaatct ggttgtcatg atttgtcagt gttccgccgt tcacggtttc tcccgcctgt
    11881 acaatcgtgt cagcagccag tgccgggact gatgtgacag cagcaagaga cagcacaacc
    11941 gccacaccgg cgcgttttcc ccgtgagcgg gccagttcgg aagccaccac cagggtgccc
    12001 gtaatgtgat tccataccag cctgtagctg gtgttcagat gtcgtttcat cagcttttcc
    12061 ttacagaggg tgaataaaaa agccggtacc cacaaaagtg gataccggca ggtacaaaac
    12121 agcatggtca gataaagtgg tttttcccgg acataaatgc ttcccctctc tcatccgcca
    12181 gagaagtatc agtccgttgt gtgcaattcc gttctgcatg atgaagagat tcagctgcct
    12241 tttcgcagac cattaaccac tataaacgat cgatagcaac gattgatacc tgttttatcg
    12301 atcgttttat tctattaacg acaaggcggt cacgcaagag aaacaggagg agtatgaggg
    12361 gtttgtgtca tgttttcagt tggttaacgt aatgttatca ggcatttccc tggcagactg
    12421 ttcagaagaa aatccagttc catacagcgc gggcaacaga gaccaccgtg tcacggacgg
    12481 cacgcagaac cgtacgggca acagaggcaa cacagacgct ctccgccgtg tcaaatatcc
    12541 ggtccaccgc accggtaaac tgttcacggg cctgaccccg gacagattcc gtgcgcagct
    12601 cgtcctgcag gcgggtcatc aggggactgg cggcatggtc gggaagtgct gtcattagcg
    12661 cactgaccag cgtatccagt tcccagccgg tgcgggccga tacggcaaca accggatgta
    12721 cgggccggaa cagacggaat accgcctccg ttttttcgcg aatattctgt gcctgtgcgg
    12781 gagaaggctg aataccggcc atatcccatt catggcaggg ctccgttttg tcggcctgcg
    12841 tcaccacaaa cagcacctgc tgatgtcccc ggtgcaggat gtgtcgccag aaatactcat
    12901 ccacagacag ggcacggtca tcggctttaa tcagccacag taccaggtca agttcaggca
    12961 gaatgtcacg gtacagggct tcatactctg catctctgtc ccggctctcg cccaccccgg
    13021 gcaggtcagt gataaccatg ctgtgaccat ggccactcag acggaaacgc tgcacttccc
    13081 gggtgccggc gtgaacatca ctgaccgggg tgacctcccc ctgaaacagt gcattacaga
    13141 gtgaggattt accggccccg cttttaccca taatgccaat cacgggttcg tgactggtga
    13201 gtttgcgcag atgttccagg atgtgacggg aaagtgagta aggcagggag gagagcggtt
    13261 tttcaattgc ctcaatggca tcagacggat tcatacatgt attcccggtg aaaaaagaca
    13321 aaacccccgc acaccggaaa ggtggcgggg gactcacgga gataatatat gtctgaaatt
    13381 atctggaatg t
//
LOCUS       DAJTSL010000106        13367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTSL010000106 DAJTSL010000000
VERSION     DAJTSL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.106"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(171..1094)
                     /gene="dmlR"
                     /locus_tag="OO433_002797"
     CDS             complement(171..1094)
                     /gene="dmlR"
                     /locus_tag="OO433_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCR6051211.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYLNFSLEHA"
     gene            1197..2282
                     /gene="dmlA"
                     /locus_tag="OO433_002798"
     CDS             1197..2282
                     /gene="dmlA"
                     /locus_tag="OO433_002798"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCR6051212.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMG
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            2532..4142
                     /gene="yeaV"
                     /locus_tag="OO433_002799"
     CDS             2532..4142
                     /gene="yeaV"
                     /locus_tag="OO433_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCR6051213.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVIPAGTVFKGDN"
     gene            4174..5298
                     /gene="yeaW"
                     /locus_tag="OO433_002800"
     CDS             4174..5298
                     /gene="yeaW"
                     /locus_tag="OO433_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCR6051214.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTSANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            5354..6319
                     /gene="yeaX"
                     /locus_tag="OO433_002801"
     CDS             5354..6319
                     /gene="yeaX"
                     /locus_tag="OO433_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6051215.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(6373..7488)
                     /gene="rnd"
                     /locus_tag="OO433_002802"
     CDS             complement(6373..7488)
                     /gene="rnd"
                     /locus_tag="OO433_002802"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6051216.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     SAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLTLVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7569..9254)
                     /gene="fadD"
                     /locus_tag="OO433_002803"
     CDS             complement(7569..9254)
                     /gene="fadD"
                     /locus_tag="OO433_002803"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6051217.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGVGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAVVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(9459..10040)
                     /gene="yeaY"
                     /locus_tag="OO433_002804"
     CDS             complement(9459..10040)
                     /gene="yeaY"
                     /locus_tag="OO433_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6051218.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGRSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(10080..10775)
                     /gene="tsaB"
                     /locus_tag="OO433_002805"
     CDS             complement(10080..10775)
                     /gene="tsaB"
                     /locus_tag="OO433_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6051219.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPATEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(10833..12743)
                     /locus_tag="OO433_002806"
     CDS             complement(10833..12743)
                     /locus_tag="OO433_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6051220.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            12875..13219
                     /locus_tag="OO433_002807"
     CDS             12875..13219
                     /locus_tag="OO433_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6051221.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3200 a         3554 c         3334 g         3279 t
ORIGIN      
        1 accagatagg tccagtaatt cagaaccccg tattcagcga acacattaac ctctttaatt
       61 atctttcgat catgcgcgat taaaggtgaa tatgctaacc aatctgtagc ggcttagaaa
      121 ggagaaaatc aggttttaac ccgatatcaa cccgataatt gaatcattaa tcaggcatgc
      181 tccagtgaaa aattcaggta gtgctcggca aaatactggc gtaaaaattc taccgttatc
      241 cgcactttcg ctgacgtcgc cagccttgaa acataaacgg cccagacgtt cgctggctgg
      301 taatattccg gtagcacttg cactaaatga ccactggcaa tgttttcgct aacatcccac
      361 caggaacgca gagcaatccc ctgcccatcg agacaccact gatggacaat ttctccatga
      421 ttagacgaca gtgggccggt gactttaatc gcatggtggc cttctttatt gcgtaattgc
      481 cagacaccaa aaggatgatc gcgctcttta atgaccaggc acggcaatgc agaaagatcg
      541 gttaaatgtt tcggcgcgcc atgttgcgca ataaattccg gcgacgcgca gagaatgcgg
      601 taattggtcg ccagtttgcg ggcgattaaa ttaggggcga tgtcatcgcc aatgcgaata
      661 tcgagatcga caccttcatt gaccaaatcg accagccggt cttccacatc aaaacgtaat
      721 tccagttgcg gatacgcctt cgccagcgct gataacgccg gagccaccac ctgtcgtcca
      781 aaaccaaagc tgctgataat acgcagcatc ccctgtggca cctgacgcac gtcagaaagt
      841 tcgtccatca tctgaccgac atcctgcaaa atccgctgcg cccattcata aatccgctct
      901 ccttcttcgg taatggtgac gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt
      961 tgctccagca aggcgatgcg cttgctgacg aacgccggtg aaacgcccag ttcttccgcc
     1021 acggcggcaa aaccagcccg acgagccacc agcataaaga cgcgcaaatc attcagcagc
     1081 ggtaaattat tcatgattcg tgttttatgt ttcaccagtt acggggatta attccttttc
     1141 cgtcaattat aggatggtga tgttgtcaat tttgatggtc aggaagtgag aacccaatga
     1201 tgaaaacgat gcgtattgct gcgatcccgg gagacgggat tggtaaagaa gtccttcctg
     1261 aagggattcg cgtgttacag gctgccgctg agcgctgggg cttcgacttg agttttgagc
     1321 aaatggggtg ggcgagctgc gagtattaca gccatcacgg taaaatgatg ccggacgact
     1381 ggcatgagca acttagccgt ttcgacgcca tctattttgg tgccgtcggc tggccggata
     1441 ccgttccgga ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc
     1501 agtacatcaa cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa
     1561 aacagcctgg cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc gaatattcct
     1621 cgctcggcgg tagagtgaat gaaggtacag agcatgaagt cgtcattcag gaatcggtat
     1681 ttacacgtcg tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg caaagccgcc
     1741 cacgtaagac gctaacttat gccactaaat cgaacggttt agccatcagc atgccgtact
     1801 gggatgagcg agtggaagca atggccgaga attacccgga gatccgctgg gacaagcagc
     1861 atattgatat tctctgcgcg cgttttgtga tgcagccgga acgcttcgat gtggtggtgg
     1921 cgtccaattt gtttggcgat atcctttccg atcttggccc ggcctgcacc ggcaccattg
     1981 gcattgcccc atccgccaac ctgaatccgg aacgcacttt cccgtcgctc ttcgagcctg
     2041 tccacggttc cgcgccggat atctacggga aaaatattgc taaccctatc gccacgattt
     2101 gggccggggc aatgatgctc gattttctcg gcaatggcga tgagcgtttc cagcaagcgc
     2161 ataacggtat tctggcagca attgaagaag tgattgctca cgggccgaaa acacctgata
     2221 tgaaaggcaa tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt
     2281 aaggtcaaac cagtttattt gaaccgcgtc actgacgcgg ttttttattc gttctttgca
     2341 gtaaataacc tgcgtcattt caccttttat tgtttccgtt tcgtgtttta tggctttccg
     2401 cattcttaat tgtttaattt atgtaatatt caaataatca atttgttaaa cacaacatac
     2461 aactcattac cctgcaaata attacaactg gcaaaagcca gagacgggct tttatgccca
     2521 ggagatgact catgatgagc aatgttaaga aaaaagatgt gccgctgata agcatcagcc
     2581 tggtggccat tcttttcatc gcagctgcat taagcctttt cccacaacaa tcggccgacg
     2641 cggccaacgc aatatacact tttgttacgc gtacgttagg ttccgccgta caggtattgg
     2701 ttttgctggc aatgggactg gtgatttatt tagccaccag taaatacggc aatattcgtc
     2761 ttggcgaagg aaaaccggaa tacagcacgc tctcctggct gtttatgttt atttgtgccg
     2821 gtttaggttc ttctacgctt tattgggggg ttgctgaatg ggcctattat tatcagacac
     2881 ctggattaaa tatcgcaccg cgttcacaac aggcactcga atttagcgtt ccctactctt
     2941 tcttccactg gggcatcagc gcctgggcaa cttatacgct ggcctcatta atcatggctt
     3001 atcactttca tgtgcggaaa aacaaaggtc tgagcctttc cggcattatt gctgctatta
     3061 ccggcgttcg cccgcaaggc ccatggggaa aactggttga tttgatgttc ctgatcgcca
     3121 ctgtcggcgc actgactatt tcccttgttg tcaccgcagc aacctttact cgtggacttt
     3181 ccgcactgac aggtttaccc gacaacttca ccgtgcaggc atttgtgatc ctgctttctg
     3241 gcggcatttt ttgcctaagc tcgtggattg gtatcaacaa cggtttgcaa cgtctgagca
     3301 aaatggttgg ctggggcgcg ttcctgctgc cattactggt gctgattgtc ggcccaaccg
     3361 aatttattac caacagcatc atcaatgcca tcggcctgac cacgcaaaac ttcctgcaaa
     3421 tgagcttatt caccgatccg cttggcgatg gttcatttac ccgcaactgg accgttttct
     3481 actggctgtg gtggatctca tacacccctg gcgtagcaat gtttgtcacc cgcgtttccc
     3541 gcggtcgtaa gattaaagaa gttatctggg gactgatcct cggcagcacc gtcggttgct
     3601 ggttcttctt cggcgtaatg gaaagctatg ccattcatca gtttatcaat ggcgtaatca
     3661 acgtcccaca ggtgctggaa acactgggcg gcgaaacagc tgtacagcaa gttctgatgt
     3721 cgttgccagc cggtaaattg ttcctcgccg catacctggg cgtgatgatt attttccttg
     3781 cctcgcatat ggatgcagtg gcctacacca tggcggcgac cagtacgcgt aatctccagg
     3841 aaggtgacga tcctgaccgt gggctgcgtc ttttctggtg cgtggtgatc actctgatcc
     3901 cgctttccat cttgtttacc ggtgcttcgc tggaaacgat gaaaaccacc gtcgtgctca
     3961 cagcccttcc cttcctcgtc attttactgg tgaaagtcgg cggatttatt cgctggctga
     4021 aacaggatta cgccgacatt ccggctcatc aagttgaaca ttatctcccg cagacaccgg
     4081 ttgaagccct ggaaaaaaca ccagtgatcc ctgcgggaac cgtattcaaa ggcgacaact
     4141 gagcgccatc atcctaacga taaaggtatc cctatgagca atctgagccc tgactttgta
     4201 ctacccgaaa atttttgcgc taacccgcaa gaggcgtgga ccattcctgc ccgtttttat
     4261 accgatcaga acgcgtttga acacgaaaaa gagaacgtct tcgccaaaag ctggatttgc
     4321 gtcgctcaca gcagcgaact ggcgaatgcc aatgattatg tgacgcgtga gatcattggc
     4381 gaaagcatcg tgctggtacg cggtcgtgat aaggttttgc gcgcgttcta taacgtgtgt
     4441 ccgcaccgtg gtcatcagtt gttgagcggt gaaggaaaag caaaaaatgt gattacctgc
     4501 ccgtatcacg catgggcatt caaactcgat ggcaatctgg cccatgcacg taactgcgaa
     4561 aacgtcgcca atttcgatag cgacaaagcg caactggttc cggtgcgtct ggaagaatat
     4621 gccggattcg tcttcatcaa catggacccc aacgccacca gcgtagaaga tcagttaccc
     4681 ggtctgggcg cgaaagtgct ggaagcctgc ccggaagtcc acgatctgaa actggcggcc
     4741 cgctttacca cccgcacgtc tgccaactgg aagaacattg tcgataacta tctcgagtgc
     4801 tatcactgtg gtccggcgca tccaggtttc tccgactccg tacaggttga tcgttactgg
     4861 cacaccatgc acggtaactg gacgctgcaa tacggtttcg ccaaaccgtc cgaacagtcg
     4921 tttaaatttg aagagggtac ggatgcggca ttccacggtt tctggctgtg gccgtgcacg
     4981 atgctgaacg tcaccccgat caaagggatg atgacggtca tttatgaatt cccggtggat
     5041 tctgaaacta ccctgcaaaa ctacgatatt tacttcacca atgaagagtt aaccgacgag
     5101 caaaaatcgc tgattgagtg gtatcgcgat gtgttccgtc cggaagattt acgtctggtt
     5161 gaaagcgtac agaaagggct gaaatcgcgt ggctatcgtg gtcaggggcg catcatggcc
     5221 gacagtagcg gtagtggcat ttccgaacat ggtatcgccc atttccataa tctgctggcg
     5281 caggtgttta aggactaatg acatcggcgg cggtattttc cgctgctggg ctgatttttg
     5341 atggagtaca gcaatatcag actatcaaat gtttgaagta caggtgagcc aggttgaacc
     5401 ccttaccgaa caggtgaaac gcttcacgct ggtggcaacc gatggcaaac cgctgcctgc
     5461 gttcaccgga ggaagtcacg tcattgtgca gatgagcgat ggtgataacc agtacagcaa
     5521 tgcgtattca ctactgagtt cgccgcatga cacctcttgt tatcagattg ccgttcggct
     5581 ggaggaaaac tcgcgcggcg gttcccgctt tttgcatcag caggtaaaag tgggcaatcg
     5641 gttaacgatt tcaacgccta ataacctgtt tgcgctaatt ccctcagcca gaaagcatct
     5701 gtttatcgcg ggcggtattg gtatcacccc tttcctgtcg cacatggcag agctgcaaca
     5761 cagcgacgtc gactggcagc tacattactg ctcgcgaaat ccagaaagtt gcgcatttcg
     5821 tgatgagcta gtccagcatc cgcaggctga gaaagttcac ttgcatcact catcaaccgg
     5881 aacacgactg gaattagcgc gattattggc ggatatcgaa cctggcacac acgtttatac
     5941 ctgtggtccc gaggcgctaa atgaagcggt aagaagtgaa gctgcgcgtc tggacatcgc
     6001 cgccgatacg ctgcactttg agcaatttgc tatcgaagac aaaaccggcg atgcctttac
     6061 cctggtgctt gcccgttccg gaaaagagtt tgtggtgccg gaagagatga ctattttgca
     6121 ggttattgaa aataataaag ccgcgaaagt ggaatgttta tgtcgtgaag gggtatgcgg
     6181 aacctgcgaa acagcaatac tggaaggtga agctgaccat cgggatcagt attttagcga
     6241 tgaagagcgt gccagccagc aaagtatgtt gatctgttgt tcgcgtgcga agggtaaacg
     6301 cctggtgttg gatttgtagt ttgccaaagc cggatgtggc gctgagcgcg cccagtccgg
     6361 cttcggaaga ttttactgcg gatattcctg caataaattg tgtaatgctt ccgccatcag
     6421 ctcaccacgc cagccggaaa tcagctccgg caaattgttc tgcggtttca gtttccagtg
     6481 ccagttcagc agttggttga tttgccgacg cgatgccagc aattcggcgc tgatcttatg
     6541 cgtttcgctc acgtcagtaa tcagcgactt aatcgcttta aacgctttac gataaccagg
     6601 catgtccatc aggttaagca tcggctgcgg taaggcctct tccggcaatg tctgcgcttt
     6661 ttccaccagc gtcagcagcg ttttaccgtg aaagcggatt tcgctaccgg ataaacccag
     6721 gctgtccagt tcgcctaaac ttcccggcat ataacgcgcg accgaccaca aatgctcttc
     6781 acgcacgaca aagttcaccg ccagatcacg ctctcgcgcc ttgcgcagtc gccagtcggc
     6841 taacagttgc agacaggcca gttggcgtgt gcgtaactgc caggcattgg tgatatcacg
     6901 ccaggcatct tccggcgcaa cgacttcctg acgacgcatt tgcatcaggc ggcattcatc
     6961 cagcgccgca ggtagccagc cggaggcctc cgtttctacc ataagcttgg cggtgatcgg
     7021 taacagatac cagacatccg ccgctgcgta ttcacactga cgttcggtca gcggtctggc
     7081 cagccagtcg gtgcgcgatt cactcttgtc cagcgtaacg ccggaatact cttccaccat
     7141 ggaagcgaaa ccccatgaca tcgggcgtcc gcagaaggca gaaaggattt gcgtgtcaat
     7201 caagggttgt ggtaattcgc caaagacatt gaggaacact tccagatctt cactgcctgc
     7261 atggagaaat tttgtgatgg acggatcgcg caggatcgct ttcagcggtg accagtcggt
     7321 gatcccgaga ggatcaatta gcgccagatg ctcgccatcg aaaagttgaa tcaaccccag
     7381 ctgcgggtaa taagtgcgcg tacgaacaaa ttcagtatcc agggctatcg ccggaaaggc
     7441 acggacggct tcacacaaag aagccagcgc atcgtccgtg gtaatcattt ggtaattcaa
     7501 attgttttct ctttagtggg cgtcaaaaaa acgccggatt aaccggcgtc tgacgactga
     7561 cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc
     7621 aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag cttcggtact
     7681 ttgtatcccg tgagctgacg gcggcaaaaa gtcaccagtg actcttcggt aagcgatgga
     7741 tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg tacgccaaca
     7801 gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc gttgggatag
     7861 acgttaaaac cggaaaccag aatcatgtct tttttacgat cgacaatgcg caggaatcct
     7921 tcttcatcca ttaccgcgat gtcgccggtg tgtaaccagc catttttgat gatttcatcg
     7981 gtagcatcgg gacgctgcca gtaacccagc atcacctgcg gtcctttgac acaaagctca
     8041 cccggttgac ctggtggtac ttcattatca tcatcatcca ccagtttggc ttccgtcgac
     8101 ggcaccggca aaccgatgct accactatga taatcaatat catatgggtt aacgctgacc
     8161 agcggcgcac actcggtaag gccatagcct tccagcagat actgtccggt cagtttcacc
     8221 caacgctctg ccaccacttg ctgcactggc atcccaccgc ctgcggaaag atgcagactg
     8281 gagaaatcca gctgctggaa ctctttattg ttcagcaacg cattgaacaa ggtgttaacg
     8341 ccagtgatag cggtaaacgg atatttcgct aactctttta ccaaccctgg aatatcacgc
     8401 gggttagtga taagcaggtt ctgcccaccc agttcgataa acagcaggca gttaatggtc
     8461 agggcaaaaa tgtgatacag cggcagtgcc gttactacca gttctttacc tggatgcagc
     8521 aacggcccat aggtggcgtt gacctgttcc aggttcgcca gcatattgcg gtgagtcagc
     8581 atcgcgcctt tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc taaatcttcc
     8641 ggcaccagtt cgggtttgac gtactgcatc cggtagccgt tatgcagtgc gctacgaaat
     8701 gaaatggcat ctggcagatg gtatttcggc accaaacgct tgatgtattt aacaacgaaa
     8761 ttgactaccg tgccttttgc cgtagatagc tgatcgccca tacgggtcag aattacgtgc
     8821 tgaacggcgg ttttatcaac cactttttcc agtgtgtgag caaagttaga cacgataaca
     8881 accgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt atacaacggg
     8941 ttaacgttta cgacgatcat cccggcacgc aaaatgccaa acagcgccac cggatattgc
     9001 aataaattag gcatcatcaa cgcaacgcga tcgcctttct tcagccccac cccttgttgc
     9061 aaataagcgg caaacgcgcg actgcgttct tccagcttgc ggaaggtcat cacctccccc
     9121 atattcacaa acgcaggctg atcggcgtag cgcgcgaccg actgctcaaa catatctacc
     9181 agagattgat aacggtcagg gttgatctcc gtcggaacgt ccgcgggata acggttaagc
     9241 caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaaat
     9301 gatatacatg ccgttaacat aatattaact catcatacca gcttgataat tacccaacga
     9361 aaaggttgcg aagcgcgtca ctatttattt ttatctttac cgtaagaatg cagaaacagc
     9421 ggaccagccg ctgtttcttt ttctttaaaa acaagcaatt attcagttac aactgtttgt
     9481 actctcgcgg gaccgggatt ataccagccc catccgccgt agccataggg ccagccacga
     9541 ccgccataaa accatggatc aatcggctga ggcggcataa tcacctgctg ggttaaatgc
     9601 caacgtttgt aacccgttac ttgcatcacc ataaatttat agggcgtatt gccgattttg
     9661 ccgtcaaccg caccagtgat tggcccgact accgtaacca gttgtccacg gaaatccacc
     9721 gggtccagaa aaccgttcac atcggcataa atgcgaccgc gagaaggttc tcccagcgtc
     9781 ggtctggctc cgctgtccag cggtacggta gcaatttcca ggcgggtttt cccttgctgg
     9841 ttttgtaccg caaccacttt gccaccaaag cgtgcctcct gaccaacgta cagctgcggc
     9901 gcactcatca cccgaactaa atcttgttgc ggcgtgggac tgcggccttt aatggcgtcc
     9961 ggcacagtga cacaaccgct cagcattagc gcaaacgtac ctgccagtat gcctttgata
    10021 acattctttt gaaccgccat gatgcgactc ctttttttct cagagcatac tcttaagatt
    10081 cattctttgc ccggaagttt cttccatgcg acgttgttac gtaaataaac cggttcggca
    10141 tgttccaccg ccaccgtttt accctcggca aatatctgac acgcaatcgg cagcatatct
    10201 tccgtcgctg gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc tttaccgaga
    10261 tccggccagg cttgccagcc cgtccctacc gttacccatt cgccggaaag ctgttgcatt
    10321 cgttcatgaa cgagttcggg tttgagtacg gcttcggttt cttcaccgcg ccagataccg
    10381 ttttcatcac gctgatattc ggcccagtaa acttcgccca tccgcgcatc aatggcagcc
    10441 agcacgcggg ttgcgccgtt tttgcgccac gccccttgcg ccattgtcat tagcgtggag
    10501 acgccaatca tcggcagctc tgcgccaagt gccagccctt gcgcaatgcc aataccaatg
    10561 cgcacgccgg taaagctacc ggggccgcgc ccgtaagcca gagcgttaat atcagtcagg
    10621 gaagttccgc tggtggtcag gatatcctgc accatcggta agattcgttg agtatgttca
    10681 cgagggcaaa gctcaaaatg agcgttgaca gtaccgtcgt tccacagggc gacagagcag
    10741 gcctctgtcg cggtatcgat agccagaatt cgcatgggtc ttcgtgctta gatcaataaa
    10801 aaggcgcgca tcataccata ctccgtaaca aattacctgg aggatggtat cgcaaggaaa
    10861 cgaaccgcac gggcaatgtc acgggtgcgc ggcgcgggcg gcagactggc gagaaacgtc
    10921 gcgccgtaag ggcgcatcac cagccgattg tcacaaatca ccaaaacccc gcgatcgtcg
    10981 gcgtcgcgaa tcagtcgccc taccccctgc ttgagagtaa tcaccgcatc cggcagttgt
    11041 acttcatcga acgggtcgcc accgcgcaaa cggcaatctt ccatgcgcgc ttttaacagc
    11101 gggtcatccg gtgaggtaaa cggcaacttg tcgataatca ccaatgacaa tgtatcgcca
    11161 cgcacgtcca ccccttccca gaaactactg gttgccacaa gaagcgcgtt accggcgctg
    11221 acaaattgct gcaacagttg ccctttgctg gtttccccct gcaacaaaac gggaagcgtc
    11281 atggtagcgc ggaactgctc ggccagatca cgcatcatgg cgtgcgaggt acaaagcata
    11341 aaacaacgac cgttgttagc ttcgatgatc ggtcgcagca ttgccgctaa ctggcgagca
    11401 gaacctggct ggttggtttg cggcagattg cgcggcacac agagtaacgc ctggcggctg
    11461 taatcaaacg ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg
    11521 aaatgatgca gatcgtcgtt caccgacagc gttgctgagg tgaagatcca gctaccaggc
    11581 ttttgcgcca ttaactcttt gaatttatcc gccacgctga gcggcgtgag agccagagta
    11641 aaatggcgcg aagtgcattc gtaccagtag ctgtagcccg gctgattgat ctcttttagc
    11701 cgcttcagcc gtgtgcgata caacgtagcg cgctcaaatg ccgcatccag caaggcggaa
    11761 cgccccagtg acagtttcgc cacgtcataa caaagttcca gggtgtcatc gagcagtaaa
    11821 aatgcccgct gaatttgcgg attagctaac agctcacgca ggttaccgcg ataacctggc
    11881 tcaccgagtt gcagacgaaa atcctgcgca ctctgggcaa gacgatcagc gcacttttgt
    11941 aactgctggg tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg
    12001 agcagttgtc gactggagag tgactgacca aaatactggc tggcaatgtc cggtagctgg
    12061 tgggcttcgt cgaagatcat gacgtccgct tccgggatca gttcgccaaa tccactctct
    12121 ttaaccacca tatccgccag aaagagatga tggtttacca ccaccacatc ggcgtccatc
    12181 gcttttttac gtgctttgac cacaaagcaa tctttataca tcgggcagtc gctgccaaga
    12241 cagttgtcgt tggtgctggt aaccagcggc cacgcctgtg aatcttccgc cacgctgacg
    12301 caggtgctga tatcaccatc gactgtttga ttagaccagg agcgcagcag gatcacatcg
    12361 cttaagattt gtaccggcag atcgcccccc gccagcgcct gctgttcgag acgttcgagg
    12421 cagaggtagt ttgagcgccc tttcagcagc gccacgttgc ccgtatattt caatgccttt
    12481 gagactgttg gcaaatcgcg gctgtagagc tgatcctgca acgcttttga gccggtcgag
    12541 ataatgactt tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt tttgcccgta
    12601 ccggttcctg cttccaccac cagcggctgg cctttttcta tcgcctgggt gacggctacc
    12661 gccatctgtc gctgtggttc tcgcggctta aagcctggta tcgctttcgc cagctgaccg
    12721 tctggtgcaa aatcgtccgt cacactaccc cctgttgatt tgaacgggga ttatgtcagg
    12781 atgagggcgc tttcgccagt tgaagtggtg acggcaacct cacattgtgg cagtctttgc
    12841 agcacgaaaa tggaagtata atgaggacaa aatgatgact atcgttcgta tcgatgctga
    12901 agcccgctgg tctgatgtag taatccacaa caacacgctc tactacactg gtgtaccgga
    12961 aaacctcgac gccgatgctt ttgagcaaac cgccaacacg ctggcgcaga ttgacgccgt
    13021 gctggaaaaa cagggcagca ataaatcgag cattctggat gccaccattt tcctggctga
    13081 taaaaacgac ttcgcggcga tgaataaagc gtgggatgcc tgggttgtgg cgggtcatgc
    13141 gccggtgcgc tgcacggtgc aagcggggct gatgaacccg aagtataaag ttgaaattaa
    13201 gattgtggct gcggtgtaag ctttatcgaa gcaaaataag tcagacgata atttatcgat
    13261 aatactggtc ggttttacat aaatcgacca gagattaaga tattactgtt gatgctcctg
    13321 ctaattcatt atttattttt ataatttcaa ttttagctat aaagcta
//
LOCUS       DAJTSL010000107        13312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTSL010000107 DAJTSL010000000
VERSION     DAJTSL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.107"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..308
                     /locus_tag="OO433_002808"
     CDS             <1..308
                     /locus_tag="OO433_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6051222.1"
                     /translation="VLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAILLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="OO433_002809"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="OO433_002809"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6051223.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="OO433_002810"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="OO433_002810"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6051224.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3390
                     /gene="ilvY"
                     /locus_tag="OO433_002811"
     CDS             2500..3390
                     /gene="ilvY"
                     /locus_tag="OO433_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6051225.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSSEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(3387..4931)
                     /gene="ilvA"
                     /locus_tag="OO433_002812"
     CDS             complement(3387..4931)
                     /gene="ilvA"
                     /locus_tag="OO433_002812"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6051226.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRAVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4934..6784)
                     /gene="ilvD"
                     /locus_tag="OO433_002813"
     CDS             complement(4934..6784)
                     /gene="ilvD"
                     /locus_tag="OO433_002813"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6051227.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6849..7778)
                     /gene="ilvE"
                     /locus_tag="OO433_002814"
     CDS             complement(6849..7778)
                     /gene="ilvE"
                     /locus_tag="OO433_002814"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6051228.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7798..8061)
                     /gene="ilvM"
                     /locus_tag="OO433_002815"
     CDS             complement(7798..8061)
                     /gene="ilvM"
                     /locus_tag="OO433_002815"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6051229.1"
                     /translation="MMQYQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8058..9704)
                     /locus_tag="OO433_002816"
     CDS             complement(8058..9704)
                     /locus_tag="OO433_002816"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6051230.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPELQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9707..9757)
                     /locus_tag="OO433_002817"
     CDS             complement(9707..9757)
                     /locus_tag="OO433_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051231.1"
                     /translation="MNNSTKFCFSTFSTGN"
     gene            complement(9844..9942)
                     /gene="ilvL"
                     /locus_tag="OO433_002818"
     CDS             complement(9844..9942)
                     /gene="ilvL"
                     /locus_tag="OO433_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6051232.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10294..11814
                     /locus_tag="OO433_002819"
                     /pseudo
     CDS             10294..11814
                     /locus_tag="OO433_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(11839..12177)
                     /gene="maoP"
                     /locus_tag="OO433_002820"
     CDS             complement(11839..12177)
                     /gene="maoP"
                     /locus_tag="OO433_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6051233.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            12296..13135
                     /gene="hdfR"
                     /locus_tag="OO433_002821"
     CDS             12296..13135
                     /gene="hdfR"
                     /locus_tag="OO433_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6051234.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(13231..13306)
                     /locus_tag="OO433_002822"
     tRNA            complement(13231..13306)
                     /locus_tag="OO433_002822"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13271..13273),aa:Trp,seq:cca)
BASE COUNT         3093 a         3600 c         3447 g         3172 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 gcctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctggta caacaagtca
      181 gtcgaaaccg attaaataga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atattgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactgaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt tcgataaagt tgtgttctct tgtccggtac
      721 tggagccgac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcaa cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactca gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gctcgtgcag tgattcataa tatgcagact
     1201 cttcaatgat gccggaatcg accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat aagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gcgagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgcac ttcggtgcca gggcatttcg gcgcaaccat gactacggtg atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta cgtcggagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccaa atccgcctgc gggatcagtt cttcgtaagt gcccacttta aaaccgtttt
     2101 cggtcgcttt acgccaggat gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacattgagg gctgctccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtgacgctg actgaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcag ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgcca
     2821 ccgattctcg accgcttccg cgcggaacac ccgtcggtgg agatcaaact aaccactggt
     2881 gatgcggcag atgcgatgga aaaggtagtc actggcgaag cggatctggc gattgccggg
     2941 aaaccggaga ctctgcccgg cgcagtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgcgc ccgcgctgcc ctgcccggtg cgtaaccagg tttcgtcaga gaagccggac
     3061 tggtctacag tgccgtttat catggccgat cagggaccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggcgtggcgc tgttgccgga agtagtgctg
     3241 gaaaacagcc ccgaaccggt gcgtaaccgc gtgatgattt tagagcgcag cgatgaaaaa
     3301 acgccgtttg aactgggcgt ttgtgcgcag aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga agattttgcc gaaccattaa cccgccaaaa agaacctgaa cgccggatta
     3421 ttggtttcgt cgtggcaatc gtagcccagc tcatttagcc gggtttcgaa atccggttca
     3481 tggtcgccga gttcgaacgc cgccagtacg cgcccgtagt cggtgccatg gctgcgatag
     3541 tggaacaaag aaatgttcca gtgcgtaccc agcgtgttga ggaagcgcag cagcgcgccc
     3601 ggtgattccg ggaattcgaa gctgtagagg cgttcctgca acggatgtga cggacgcccg
     3661 ccgaccatat agcgcacatg caacttcgcc atttcgtcgt cggagagatc caccacgctg
     3721 tagccgccgt cgttgagcat ctgcaaaatt tctttgcgct cttcgaggcc gcggctcagg
     3781 cgcacgccga caaagatgca ggcgtttttg gcatcggcaa aacggtagtt gaactcggtg
     3841 accgctcgcc cgccaagcag ttggcagaat ttgaggaagc tgcctttttc ttccggaatg
     3901 gtcaccgcca acaacgcttc acgctgttcg cccagttcgc agcgttccga gacgtagcgc
     3961 aggccgtgga agttcacgtt ggcaccggaa agaatatgcg ccagccgttc gccgcgaatg
     4021 ttgtgctggg cgatatattt tttcattccc gccagcgcca gcgcgccaga gggttccgcc
     4081 accgcgcgca catcttcgaa taaatccttc atcgccgcac agatcgcatc actatcgacg
     4141 gtgatgatgt cgtcgagata ctcctggcat aaacggaagg tttcgtcacc gatgcgtttt
     4201 accgctacgc cttcagcaaa tagccctacg cgcggcagat caaccggatg acccgcatcc
     4261 agcgctgctt tcaggcaggc ggagtcttcc gcttctacgg cgatcacttt gatttgtggc
     4321 atcagttgtt tgatcagcac cgcaacgccc gccgccagac cgccgccgcc gactggcaca
     4381 aatacgcggt cgagatgggc gtcctgctgg agcagttcca gcgccagcgt gccttgcccg
     4441 gcaatcacca tcggatggtc gaacggcggc acccaggtga acccctgctg ctgtgacagt
     4501 tcgatcgctt tagctttcgc ttcgtcaaag ttcgcgccat gaagcagcac ttcgccgccg
     4561 aatccacgta ccgcatcgac tttgatgtcg gcggtggcgg ttggcataac gatcagggcc
     4621 ttcacgccta accgcgcaga agaaaacgcg acgccctgcg cgtggttacc cgcagaagca
     4681 gtgatcacgc cgtgcgcttt ttgttcttcc gtcaggcccg ccatcatggc gtatgcgccg
     4741 cgcagcttaa agctgtgcac tggctggcga tcttcgcgct tcaccagaat gacgttatca
     4801 agacgcgacg acagtttttc cattttttgt agcggcgtaa cctgcgctgc ctcgtaaacc
     4861 ggcgcgcgca gcactgctct taaatattcg gcaccttccg gagcaccaga caggggttgc
     4921 gagtcagcca ttattaaccc cccagtttcg atttatcgcg caccgcacct ttgtcggcgc
     4981 tggttgccag gctggcgtag gcgcgcaggg caaaggaaac ctgacgttca cggtttttcg
     5041 gcgtccaggc tttgtcaccc cgagcttcct gcgcttcacg acgcgccgcc agttcggcat
     5101 cgcttacctg taactgaatg ccacggttcg ggatgtcgat agcgatcagg tcaccatctt
     5161 caatcagacc aatgctgccg ccgcttgccg cttccggtga gacgtggccg atggaaagac
     5221 cagaggtgcc gccagagaaa cgaccgtcgg tgatcagcgc acaggcttta ccgagaccca
     5281 ttgatttcag gaagctggtt gggtagagca tttcctgcat gccagggccg cctttcgggc
     5341 cttcatagcg gatcaccacc acatcgcccg cgacaacttt gccaccgagg atcgcttcta
     5401 ccgcgtcgtc ctggctttcg tacacttttg ccgggccggt gaatttgagg atgctgtcat
     5461 cgacgcccgc ggttttcacg atgcagccgt tttcagcaaa attaccgtag agcaccgcca
     5521 ggccgccgtc tttgctgtag gcgtgttcca gcgagcggat acagccgttt gcgcgatcgt
     5581 cgtccagcgt atcccaacgg caatcttgcg agaatgcctg ggtggtacgg atacccgcag
     5641 ggccggcgcg gaacatattt tttaccgcgt catcctgggt cagcataacg tcgtattgct
     5701 ccagcgtttg cggcaacgtc aggccgagca cgtttttcac atcacggttc agtaaccccg
     5761 cgcgatccag ttcgccgaga ataccgataa caccaccagc acggtgaaca tcttccatat
     5821 ggtatttctg ggtgctcggc gcaactttac acagctgtgg aaccttgcgg gaaagcttat
     5881 cgatatcact catggtgaag tcgatttccg cttcctgcgc cgccgccagc aggtgaagta
     5941 cggtgttagt cgatccaccc atcgcgatat ccagcgtcat ggcgttttca aacgccgcct
     6001 tactggcgat attacgcggc agtgcacttt cgtcgttttg ctcgtaataa cgtttggtca
     6061 attcaacaat gcgtttacca gcattaagga acagctgctt acggtccgcg tgggttgcca
     6121 gcagcgagcc gttgcctggc tgcgacaaac ccagcgcttc ggtcaggcag ttcattgagt
     6181 tagcggtaaa catcccggag caggaaccgc aggttgggca ggcggaacgt tcaacctgat
     6241 cgctctggga gtcagaaact ttcgggtctg cgccctggat catcgcatca accagatcga
     6301 gtttgattat ctgatcggac agtttggttt tcccggcctc catcgggccg ccggaaacaa
     6361 agatcaccgg aatattcagg cgcagggaag ccatcagcat ccccggggtg attttgtcgc
     6421 agttagagat gcagaccatg gcgtcggcgc agtgggcgtt gaccatatac tcaacggaat
     6481 cagcgatcag ttcgcgagat ggaagtgaat aaagcatccc cccgtggccc atggcaatcc
     6541 catcatccac cgcaatggtg ttgaactctt tggcaacgcc gccagccgct tcaatttgtt
     6601 cggcgaccag tttaccgaga tcgcgcagat ggacgtgacc cggtacaaat tgggtgaacg
     6661 agttcacaac cgcgataatc ggcttgccaa aatcggcgtc ggtcattccg gtggcgcgcc
     6721 acagcgcacg agcacccgcc atattacgac catgagtggt ggtggcggaa cggtacttag
     6781 gcatacttta tttactccca gtgtctgtct cgtaaatggg acggtgcgtg ccgtcccatt
     6841 ttttgtattt attgattaac ttgatctaac cagccccatt tatcttcggt ttcgccagtg
     6901 aagaggccga agaaggcttg ctgaatgcgt ttggtaaccg ggccacaacg gccttcgcca
     6961 acctgaatac cgtctacgct gcgcactggc gtgatttctg ccgcagtacc ggacataaac
     7021 acttcatccg ccaggtacag ggattcgcgc gacagcacct gctcacgcac ttcaattccc
     7081 agctctttcg ccagtttgat gatggcatca cgggtaatac ccggcagcgc ggatgaggta
     7141 aacggtgggg tgaacagcac gccgtctttc acttcaaaca ggttttcacc tgcgccttca
     7201 gagatgtaac cgttcacatc cagcgcgata ccttcctgat aaccgtggcg gcgcgcttcg
     7261 ctacccacca gcagggaaga gaggtagtta ccaccggctt ttgccgccgt cgggatggtg
     7321 tttggtgctg cgcggttcca ggaggaaacc atcgcatcga tcccctgctc cagcgcttct
     7381 gcgcccagat acgctcccca cgggaaagca gcgataatca cgtcggttga gtatcccgct
     7441 ggcgggttta ctcccatgcc aacatcaccg acgaagatca gcggacggat ataggcgctg
     7501 gtgagattat ttttgcggat cacgtcacga caagcttcca tcagctcatc aatgctctgc
     7561 gaaaccggga agcgatagat tttggcggag tcatgcagac gctgcatatg ctcacgatgg
     7621 cggaatacaa ccggtccttt gtgcgagtcg tagcaacgga tgccttcaaa aaccgaagtg
     7681 ccatagtgca gcgcgtgcga catcacatgc accttcgcgt cttcccagcg aaccatctcc
     7741 ccattgaacc aaatgtaatc agctttcttc gtggtcattt ttatattcct tttgcgctca
     7801 ggcgcggatt tgttgtgatg tggttgtgct ctggcagatg gcaacgtgtg cgacgtccac
     7861 cagtttattt aactgactaa acagtaagtc gaccgaccgt gggctggcaa cggtcaattc
     7921 gatatttata ttttgtgcat cgctggcggc ggccatattc attgagcaga cgtggaaacc
     7981 acgatgacgc accacgcgta aaacacgttc taaggtttcc ggattgaagc gagccgatac
     8041 attgacctga tattgcatca tgataatttc tccaacattt ctgaattact ggcgccaggc
     8101 ggcaccagcg gccagacgtt ctcaagttcg tcgattgaga catgaagcag gtatggccca
     8161 tcactgttca gcatggtgtc gagtgccgct tcaacctggt ctttacgggt gatgtgttgg
     8221 ccagggatgc cgaaggcgct ggctaacatg aggaaatcgg ggttatcagt aagggtggtt
     8281 tcgctgtatc gttcctgaaa aaacagttgc tgccattgtc gaaccatccc taaccgttgg
     8341 ttatcgagta agacgatttt caacggtaac tgcttgcgtt ttacggtgcc cagctcttgc
     8401 acattcatca tgaaagagcc gtcaccggag atacagacga cggtatcgtt cggtcgcgcg
     8461 acttgtgcgc caaccgccgc tggtaaacca aaacccatag tgcctaagcc gctggaggtg
     8521 atgaaatttt ccgggcgagt gtgggcgatg tgttgcgcag cccacatctg gtgctgcccc
     8581 acatctgtgg tcacgacgca atccgcaggt ttacgatccg acagttgttt taacaacaac
     8641 ggcgcgtaga tagcgtcgcc gggatggtcg taacgccagg catgttcatc acgcagctgc
     8701 gcgcagtatt gctgccagtc attgatattt aacggctgct gtaattctgg taacagagca
     8761 tttaaatcac cttgtaatgc cacatgtgcc tgacgcagct tgttcatttc tgccgggtcg
     8821 atatccatat ggataacgct ggcgtgtggt gcgaaggtgt tcagtttgcc ggtcacccgg
     8881 tcatcaaaac gtgcacccac ggcgatcagc aagtcgcact cctgcaccgc gaagttcgcc
     8941 gctttggtgc catgcattcc cagcatgccc agatagtacg gataatctgc ttcaactgcg
     9001 cccagccctt tcagcgtgca ggtggcaggc atttttgtgg tagcgagaaa ttctcgtaaa
     9061 gcaggaactg cctgcgccat acccacgcca ccaccaacgt acagcatcgg tttttgcgct
     9121 tttgccagca tctggcgcgc ttgctcaact tcggcatgtg ggaaagtcac ttcgttttca
     9181 acggtggtga accacggttc caggtcaccg ctggctagct ggatatcttt tgggatatcg
     9241 accagaaccg gaccaggacg acctgagctg gcaacgtcga atgcttcagc cataatgcgc
     9301 ggcaactctt ccagcgactg caccagaaag ctgtgcttgg tacaggctaa cgacaatccc
     9361 aggacatcca cttcctgaaa tgcatcagtg ccgataaacg gtgcggacac ttgaccggtg
     9421 atggcaacaa cagggataga atctaacagt gcgtccgcaa gcccggttat caggttggtt
     9481 gcgcccggac cagacgtggc gatacatacg ccagttttgc cggtagcacg ggcataaccg
     9541 atagccgcca ttgccgcacc ctgctcatgt cggcacagca agtgctccac gccgccgtca
     9601 tacaatgcat cgtaaaccgg cataattgcg ccacccggat aaccgaaaac ggtgttcaca
     9661 ccctgtgccc gcaacgcatg taccacccac tgtgcgccat tcatagttag ttccccgtgc
     9721 tgaatgttga gaaacagaat tttgtgctgt tattcattgt ctgctcctcg gttatgtttt
     9781 taaggtcaaa aaaaaccccc ggacctttcg gtgcgggggt cttagttcgt taaggcttga
     9841 tttctaagcc tttcctcgtc caagtgcagc cccgcacggt gggataataa tcaccaccac
     9901 gctaatcacg accaggctaa tcactcgtag aagggctgtc attttgtctt ttcttgcatc
     9961 ttgttcgaag gaatgcctaa agagttacca taggttttgt aaatagtaca agatattttt
    10021 tggccgattt ttatgatgcg tttaataatt tcaatataag ttgttgtttt ttaaaaataa
    10081 aatagtgagt aaaaaaattc aatttcagta tgaatgttgt tcccttcctc gtagttcatc
    10141 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10201 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10261 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10321 gcagccctgg gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta
    10381 cccggcttaa cgaaggtggg cttaccagaa acaacggtaa aagaagctcg cgatcgcgtg
    10441 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctg
    10501 gctccagctg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10561 ctggcggcct cagaacagct tacagccaat aagttagatg aatatggatt agtcggagaa
    10621 ctggcgctta caggcgctct gcgtggcgtt cccggcgcaa tctccagtac aactgaagct
    10681 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10741 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10801 aagcacgctc tcgaacgccc gaaaccaact gatacagtat cccgggcgct acaacatgat
    10861 ctcagtgatg ttatcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10921 gggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    10981 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagtgc tgcgatatta
    11041 agtctggtaa acgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11101 catcacagtg catcgttaac tgcgatggta ggcggtggcg caattccagg gcccgatgaa
    11161 atttcgctgg cgcataacgg cgtgcttttt cttgatgagt tacctgaatt tgaaaggcgt
    11221 acactggatg ccttgcgaga gccgattgaa tccgggcaga tccatctttc acgcacgcga
    11281 gcaaaaataa cctatccagc ccgtttccag cttgtcgcgg cgatgaatcc cagccctacc
    11341 ggacattatc agggaaacca taaccgctgc acaccagagc aaacgctgcg ttatctcaac
    11401 cgactctctg gtccctttct cgaccgcttc gatctctcac tggagatccc attaccgcct
    11461 cccggtattt tgagtaaaac ggtagtgccg ggagaaagca gcgccaccgt taaacaacgc
    11521 gtaatggccg ccagagagcg ccaatttaag cggcagaata aactgaacgc ctggctggat
    11581 agtccggaaa tacgccaatt ctgcaaactt gagagcgaag atgcgatgtg gctggaagaa
    11641 acgctgatcc atctggggtt atcgattcgt gcctagcagc ggttattgaa agttgcacga
    11701 accattgctg atattgatca gtctgacatt atcacacgtc aacatttgca ggaggcagtt
    11761 agctatcgag cgattgaccg tttgctcatc catctgcaga aactactgac ataaaaaaag
    11821 ggcatttcgc cctttttatt aatcgtcaga atcggtgtag tcttcagcac cttcaacctg
    11881 cggtttaccg ccggaaaggg tgtgaaaacg ttttggacgc ttaatacgcg tcatatactt
    11941 ggaccacacg cgttctgctt ctgtcactgg ctcacgttcg ccacggcata ctgctacgaa
    12001 gagtttctct tcctcggtaa ccggctcgcg tttgccaaga tccaactcat tgaaggcata
    12061 accatgacgc tcaagcagtt gtgcctcttt gatggtgaaa tcaccatgac gagagaatcc
    12121 acgtggataa tgtttattgt cgaaatatcg attagtcgtc gtaaagcttt ccgccatcct
    12181 gcacgctcct aattctttga ccgagctagt tatggcgcgg agtattagtt acgcttgaca
    12241 gagtgtaaaa caaaacattt aaatcataac gacaaataat tttgcggaga gcactgtgga
    12301 tacggaattg ttaaaaactt tcctggaagt tagccgaacg cgtcactttg gtcgagcggc
    12361 tgaatcgctc tatctgaccc agtcagcagt gagctttcga atcagacaac tggaaaatca
    12421 actgggtgtg aaccttttca cccgccacag aaacaatatc cgtttaaccg ctgccggtga
    12481 aaaactactg ccttatgcag aaacgctcat gagcacctgg caggccgccc gcaaggaggt
    12541 ggcgcatacc tcacgacata acgagttttc tatcggtgcc agcgcctcat tgtgggaatg
    12601 tatgcttaat cagtggctgg gacgtttgta tcaaaatcag gatgcccata caggcttaca
    12661 gttcgaagcg cgaattgccc aacggcagtc tctggtaaaa cagctgcatg aacgccagct
    12721 tgatcttctt attaccactg aagcgcccaa aatggacgaa tttagtagtc agttgctggg
    12781 atatttcact ttagcgcttt ataccagtgc cccttcaaaa ctaaagggag atcttaatta
    12841 tctgcgactt gagtgggggc cagattttca acagcatgag gcaggtttga tcggtgctga
    12901 cgaagtgccc attctgacaa ccagttctgc tgaactggca cagcaacaga ttgcgatgct
    12961 taatggttgc acctggctac ccgtcagctg ggcgcgtaaa aaaggcggcc tgcataccgt
    13021 tgtcgatagc acaacacttt cacggccgct ttatgccata tggctgcaaa atagcgataa
    13081 aaatgcgttg attcgcgatc ttttgaaaat taacgtgctg gatgaagtgt attaatatga
    13141 atggctggca aggatgccgg tagaaggatt tacttcggag agggttattt cagataaaaa
    13201 aaatccttag ctttcgctaa ggatgatttc tggcaggggc ggagagactc gaactcccaa
    13261 cacccggttt tggagaccgg tgctctacca attgaactac gcccctaatt ag
//
LOCUS       DAJTSL010000108        13298 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTSL010000108 DAJTSL010000000
VERSION     DAJTSL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.108"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            17..352
                     /locus_tag="OO433_002823"
     CDS             17..352
                     /locus_tag="OO433_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6051235.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDTEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            512..955
                     /gene="yjdN"
                     /locus_tag="OO433_002824"
     CDS             512..955
                     /gene="yjdN"
                     /locus_tag="OO433_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6051236.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1088..1876
                     /gene="phnC"
                     /locus_tag="OO433_002825"
     CDS             1088..1876
                     /gene="phnC"
                     /locus_tag="OO433_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6051237.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            1901..2917
                     /gene="phnD"
                     /locus_tag="OO433_002826"
                     /pseudo
     CDS             1901..2917
                     /gene="phnD"
                     /locus_tag="OO433_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
     gene            3043..3822
                     /gene="phnE"
                     /locus_tag="OO433_002827"
     CDS             3043..3822
                     /gene="phnE"
                     /locus_tag="OO433_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR6051238.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3843..4568
                     /gene="phnF"
                     /locus_tag="OO433_002828"
     CDS             3843..4568
                     /gene="phnF"
                     /locus_tag="OO433_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR6051239.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4569..5021
                     /gene="phnG"
                     /locus_tag="OO433_002829"
     CDS             4569..5021
                     /gene="phnG"
                     /locus_tag="OO433_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR6051240.1"
                     /translation="MHADIATRQHWMSMLAHSQPAELAARLNALNITANYEVIRTAET
                     GLVQIQPRMGGTGERFFAGDATLTRAAVRLTDGPLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5018..5602
                     /gene="phnH"
                     /locus_tag="OO433_002830"
     CDS             5018..5602
                     /gene="phnH"
                     /locus_tag="OO433_002830"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR6051241.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQSELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5602..6660
                     /gene="phnI"
                     /locus_tag="OO433_002831"
     CDS             5602..6660
                     /gene="phnI"
                     /locus_tag="OO433_002831"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR6051242.1"
                     /translation="MYVAVKGGEKEIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIMPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVD
                     FQAELELLKRLQQEQNHG"
     gene            6653..7498
                     /gene="phnJ"
                     /locus_tag="OO433_002832"
     CDS             6653..7498
                     /gene="phnJ"
                     /locus_tag="OO433_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR6051243.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7495..8253
                     /gene="phnK"
                     /locus_tag="OO433_002833"
     CDS             7495..8253
                     /gene="phnK"
                     /locus_tag="OO433_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR6051244.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8364..9044
                     /gene="phnL"
                     /locus_tag="OO433_002834"
     CDS             8364..9044
                     /gene="phnL"
                     /locus_tag="OO433_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR6051245.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9041..10177
                     /gene="phnM"
                     /locus_tag="OO433_002835"
     CDS             9041..10177
                     /gene="phnM"
                     /locus_tag="OO433_002835"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR6051246.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLNAVAIGDVR
                     DGGDQLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFTTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10177..10734
                     /gene="phnN"
                     /locus_tag="OO433_002836"
     CDS             10177..10734
                     /gene="phnN"
                     /locus_tag="OO433_002836"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR6051247.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLKNRGRENTSEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10721..11170
                     /gene="phnO"
                     /locus_tag="OO433_002837"
     CDS             10721..11170
                     /gene="phnO"
                     /locus_tag="OO433_002837"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR6051248.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PSMRYPSMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQACGLNVSSKL
                     LAWAEEEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11172..11930
                     /gene="phnP"
                     /locus_tag="OO433_002838"
     CDS             11172..11930
                     /gene="phnP"
                     /locus_tag="OO433_002838"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR6051249.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWPPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRSHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12077..12406)
                     /gene="yjdP"
                     /locus_tag="OO433_002839"
     CDS             complement(12077..12406)
                     /gene="yjdP"
                     /locus_tag="OO433_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR6051250.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12475..12792)
                     /locus_tag="OO433_002840"
     CDS             complement(12475..12792)
                     /locus_tag="OO433_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCR6051251.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13045..>13298
                     /locus_tag="OO433_002841"
     CDS             13045..>13298
                     /locus_tag="OO433_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051252.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPY"
BASE COUNT         2965 a         3981 c         3696 g         2656 t
ORIGIN      
        1 ttttgaggaa atccacatgt cattaccaca ctgcccaaaa tgcaactccg aatacactta
       61 cgaagataac ggcatgtaca tctgcccgga atgtgcctac gaatggaacg acacagaacc
      121 tgcacaggaa agcgacgagc tgatcgttaa agatgctaac ggcaatctgc tggctgacgg
      181 cgacagcgtt accatcatta aagatctgaa ggtgaaaggt agctcttcga tgctgaaaat
      241 tggcaccaaa gtgaaaaaca tccgcctggt tgaaggcgac cataacatcg attgcaaaat
      301 cgacggtttt ggtccgatga aactgaaatc tgagtttgtg aaaaagaact gattgtattg
      361 tgatcggtaa gccggataag gcgctcgcgc cacatccggc aacgatgccg actgcctgat
      421 gcgacgctga ccgcgcctta tcaggcttcc ctctcatctg tataaatttc gaactacact
      481 taactggctt ctcttaactg aggtcaccat catgccgtta agtccctacc tctcttttgc
      541 cggtaactgt tccgacgcga ttgcctatta tcaacgtacg ttgggcgcgg aactgctcta
      601 taaaatcagc ttcggcgaaa tgccaaaatc agcgcaggac agcgccgaga actgcccttc
      661 cggaatgcaa tttcccgata ccgccatcgc ccacgccaac gtgcgcattg ccggaagcga
      721 catcatgatg agcgatgcta taccgtcagg aaaagccagc tactccggct ttacgctggt
      781 gctcgattcg caacaggtcg aagaaggaaa acgctggttt gataaccttg ccgctaacgg
      841 aaaaattgaa atggcctggc aggaaacttt ctgggcgcat ggcttcggca aagtcaccga
      901 taaatttggc gtaccgtgga tgattaatgt cgtcaaacaa caaccaacgc aataacccgc
      961 cgggaggccc gccctcccgc actgtcatcg aattcccgtt aactcttcat ctgttagtca
     1021 cttttaatta accaaatcgt cacaataatc cgccacgatg gagccacttt tttagggagg
     1081 ctgcatcatg caaacgatta tccgtgtcga gaagctcgcc aaaaccttca atcagcatca
     1141 agcgctgcat gcggttgatc tgaacattca tcacagtgaa atggtggctc tgcttgggcc
     1201 gtcgggttcc ggcaaatcca cacttttacg tcacttaagc ggtttgatta ccggcgataa
     1261 atccgccggc agccatatcg agctgctggg ccgcacagtc cagcgcgaag gccgtctggc
     1321 gcgcgatatc cgcaaaagcc gcgcccatac cggctacatc ttccaacaat tcaacctggt
     1381 gaatcgcctg agcgtactgg agaacgtgct gattggcgcg ctcggcagca cgccgttctg
     1441 gcgcacctgt tttagctggt tcacccgcga gcagaaacaa cgcgcgttac aggcgctgac
     1501 ccgcgttggc atggtgcatt ttgcccatca acgcgtttcc accctctccg gcggacagca
     1561 gcagcgtgtg gcgattgccc gcgcgctgat gcagcaggcg aaggtgattc tggccgatga
     1621 acccatcgcc tcgctggacc cggaatccgc ccgcatcgtg atggacaccc tgcgcgacat
     1681 caatcagaac gacggcatca ccgtagtcgt cacgctgcat caggtggatt acgccctgcg
     1741 ctactgcgaa cgcatcgtcg ccctgcgcca ggggcacgtt ttctacgacg gcagcagcca
     1801 acagtttgat aacgaacgtt ttgaccatct ctaccgcagc attaatcgca tcgaagagaa
     1861 cgcgaaagct gcctgacatc cccatcattg aggaaaacga atgaacgcta agataattgc
     1921 ctcgctggcc ttcaccagca tgttcagcct cagcaccctg ttaagcccgg cacacgccga
     1981 agagcaggaa aaggcgctga atttcggcat tatttcaacg gaatcacagc aaaacctgaa
     2041 accgcaatag acgccattct tacaggatat ggagaagaag ctgggcgtga aggtgaacgc
     2101 cttctttgcc ccagactacg caggcattat ccagggaatg cgcttcaata aagtggatat
     2161 cgcctggtac ggcaacctgt cggcaatgga agcggtggat cgcgccaacg gccaggtctt
     2221 cgcccagacg gtcgcggcgg atggatcgcc aggttactgg agcgtgttga tcgtcaacaa
     2281 agatagtccg atcaacaacc tgaacgatct gctggcgaag cggaaagatc tcaccttcgg
     2341 caatggcgat cctaactcca cctctggctt cctcgtcccc ggttactacg tctttgccaa
     2401 aaacaatatc tccgccagcg actttaagcg caccgtcaac gctgggcatg aaaccaacgc
     2461 gctggccgtc gccaacaagc aggtggatgt tgccaccaac aacaccgaaa acctcgacaa
     2521 gctgaaaacc tccgcgccag agaagctgaa agaactgaag gtgatctgga agtcgccgct
     2581 gatcccaggc gatccgatcg tctggcgcaa gaatctctcc gaaaccacca aagacaagat
     2641 ctacgacttc tttatgaact acggaaaaac gccggaagag aaagcggtgc tggaacgcct
     2701 gggctgggcc ccgttccgcg cctccagcga cctgcaactg gtgccgattc gccagctcgc
     2761 actgtttaaa gagatgcagg gcgtaaaaag caataaagga ctgaatgagc aggacaagct
     2821 ggcgaaaacc accgagattc aggcgcagct ggatgacttg gaccgcctga acaacgcgtt
     2881 aagcgcgatg agttcggtga gtaaagcggt gcagtaatgt gctcgatcgg tcccctctcc
     2941 cttgagggag agggttaggg tgagggggaa atgcgcacgc tgatgccctc atcccggccc
     3001 tctcccacag ggagagggag aaaacattaa acggagccaa acatgcaaac catcaccatc
     3061 gccccaccca agcgtagctg gttctcgctt ctgagctggg ccgttgttct cgccgtgctg
     3121 gtcgtctcgt ggcagggcgc ggaaatggcc ccgcttacgc tgattaaaga cggcggcaac
     3181 atggcaacct tcgttgccga cttcttcccg cccgatttca gccagtggca ggattacctc
     3241 accgaaatgg ccgtcacgct gcaaatcgcc gtctggggca ccgcgctggc agtggttctc
     3301 tccatcccct tcggtctgat gagcgccgaa aacctggtgc cgtggtgggt ttaccagccc
     3361 gttcgccgcc tgatggatgc ctgccgcgcc attaacgaaa tggtcttcgc catgctgttc
     3421 gtggtcgccg tcggtctcgg cccgttcgct ggcgtgctgg cgctatttat ccacaccacc
     3481 ggcgtgctct ccaagctgct ttccgaagcg gtggaagcga tcgaacccgg cccggtagaa
     3541 ggcattcgcg ccaccggcgc caacaagctg gaagagatcc tttacggcgt gctgccacag
     3601 gtaatgccgc tgctgatctc ctactccctc tatcgcttcg aatccaacgt ccgctcggcg
     3661 accgtcgtcg gcatggtcgg tgcaggcggg atcggcgtca ccctgtggga agcgattcgc
     3721 ggttttcagt tccaacaaac ctgcgccctg atggtgctta tcatcgtcac ggtcagcctg
     3781 ctggatttcc tctctcaacg gttgcgtaag cactttatct gataagcgag gcattgatat
     3841 ctatgcactt gtctacacat ccgaccagct acccaacacg ctatcaggag atagccgcaa
     3901 aacttgagca ggagcttcgt caacactatc gctgcggcga ctatcttccc gccgagcagc
     3961 agctggcggc gcgctttgag gtaaatcgcc acaccctgcg ccgcgccatc gaccaactgg
     4021 tggaaaaagg ctgggtacag cgccgtcagg gcgtcggcgt gctggtgctg atgcgcccgt
     4081 tcgattaccc gctcaacgcc caggcgcgtt ttagccagaa cctgctggat cagggcagcc
     4141 atcccaccag cgaaaaactg ctgtcggtgc tgcgcccggc atctggtcac gtcgctgacg
     4201 cgctggggat aaccgagggg gagaacgtca tccacctgcg caccctgcgc cgggtcaacg
     4261 gcatcgcgct ctgtttaatc gaccactact ttgcggacct caccctttgg ccgacgctgc
     4321 aacgcttcga cagcggctcg ctgcacgatt ttctgcgcga gcaaaccggg attgcgctgc
     4381 gccgcagcca gacgcggatc agcgcccgcc gcgcccaggc caaagagtgc cagcgtcttg
     4441 aaatcccgaa tatgtcgccg ctgctgtgcg tgcgcaccct taaccaccgt gacggtgaaa
     4501 gcagcccggc ggagtactcc gtcagcctga cgcgcgccga catgattgaa ttcactatgg
     4561 agcactgaat gcacgcagat atcgcgaccc gccagcactg gatgtccatg ctggcgcaca
     4621 gccaaccggc tgaactggca gcccgcctga acgcgctgaa catcaccgcc aactatgaag
     4681 tgatccgcac cgccgaaacc ggcctggtac agattcagcc gcgaatgggc ggtaccggcg
     4741 aacgcttttt tgccggcgac gccacgctga cccgcgccgc cgtacgcctg actgacggcc
     4801 ccctcggcta cagctgggtg ctggggcgcg acaaacagca cgccgaacgc tgcgcgctga
     4861 ttgacgcgtt gatgcagcaa tctcgccatt ttcaaaactt atcagaaacc cttattgccc
     4921 cactggacgc tgaccgtatg gcacgcattg ccgcacgcca ggccgaagtg aacgccagcc
     4981 gggtcgactt ctttacgatg gttcgcggag acaacgcatg accctggaaa ccgcttttat
     5041 gcttcctgtg caggatgccc agcacagttt tcgtcgcctg ttaaaggcca tgagcgagcc
     5101 gggcgtgatt gtcgccctgc atcagctcaa acgcggctgg cagccgctga atatcgccac
     5161 caccagcgtg ctgctgacgc tggctgataa cgacacgccg gtgtggcttt ccgcgccatt
     5221 aagtaacgat atcgtcagcc agagcctgcg ttttcatacc aacgcgccgc tggtcaatca
     5281 gtcggaactg gcgaccttcg cggtaacgga tgaggcgatt tccagcgaac agctcaacgc
     5341 tctttccagc ggcaccgctg ttgcgccgga agcgggcgcg acgctgattt tacaggtcgt
     5401 cagcctgagc ggcgggcgca tgctgcgcct tactggcgcg ggtattgccg aagaacgaat
     5461 gatcgctccg cagctgccgg agtgcattct gcacgaactc accgagcgcc cgcacccgtt
     5521 cccgctcggc atcgacctga tcctgacctg cggcgagcgg ctgctggcta tcccgcgaac
     5581 cactcatgtg gaggtgtgct gatgtacgtt gccgtgaaag ggggcgagaa ggagatcgac
     5641 gccgcccacg ccctgcaaga gagccgacgc cggggcaata ccgatttgcc tgaactgagc
     5701 gtcgcccaga ttgaacagca gcttaatctt gcagtagatc gcgtgatgac cgaaggcggc
     5761 attgccgacc gcgaactggc ggcgctggcg ctgaaacagg ccagcggcga taacgtcgaa
     5821 gcgattttcc tgctacgcgc ctaccgcacc acgctggcga agctggcggt aagcgagcca
     5881 ctcgacacca cggagatgcg tctcgaacgg cgtatttcag cggtttataa agatattccc
     5941 ggcggtcagt tgcttggccc aacctacgac tacacccatc gcctgctcga tttcaccctg
     6001 ctggcaaacg gcgaagcgcc gacgctgacc accgcagaca gcgaacaatc cccgtcgccg
     6061 cacgttttca gcctgctggc gcgtcagggg ctggcgaagt ttgaagagga tagcggcgca
     6121 cagccggatg acatcacccg cactccgcca gtttacccct gttcacgttc ttcccgtttg
     6181 cagcagttga tgcgcggcga cgaaggctat ttgctggcgc tggcctactc tacccagcgc
     6241 ggttacggac gcaatcaccc gttcgcgggc gaaatccgca gtggttacat cgacgtgtcg
     6301 attatgccgg aagagctggg atttgcggtg aacgtcggcg aactgctgat gaccgagtgt
     6361 gaaatggtca acggttttat tgacccgccg gatgagccgc cgcacttcac gcgcggctac
     6421 gggctggtgt tcggcatgag cgagcgcaaa gcgatggcgc tggttgaccg tgcgttgcag
     6481 gctccggagt acggcgagca cgcgacaggc ccggcgcagg atgaagagtt cgtgctggca
     6541 catgccgaca acgtcgaagc cgcaggcttt gtctcgcacc tcaaactccc ccactacgtc
     6601 gatttccagg ccgaactgga gctactcaaa cgtctgcaac aggagcagaa ccatggctaa
     6661 tctgagcggc tacaactttg cctacctcga cgagcagacc aaacgcatga tccgccgcgc
     6721 catcttaaaa gcggtggcga tccccggtta tcaggtgccg tttggcgggc gcgagatgcc
     6781 gatgccctac ggctggggaa ccggcggcat tcagctcacc gccagcgtga ttggcgaaag
     6841 cgacgtgctg aaggtgattg accagggcgc agacgacacc accaacgccg tgtcgattcg
     6901 caacttcttt aagcgcgtga ccggggtaaa caccactgaa cgtacggacg atgcgacgct
     6961 tatccagacg cgccatcgca tccccgaaac gccgctgacc gaagatcaga tcattatctt
     7021 ccaggtgccg atccccgaac cgctgcgctt tatcgagccg cgcgaaacgg aaacccgcac
     7081 catgcacgcg ctggaagagt acggcgtgat gcaggtgaaa ctgtatgaag atatcgcccg
     7141 cttcggtcat atcgccacta cctacgccta tccggtgaag gtgaacgggc gctacgtgat
     7201 ggacccgtcg ccgatcccga aattcgataa cccaaaaatg gacatgatgc ccgccctgca
     7261 actgttcggc gcggggcgcg agaagcgcat ctatgcggtg ccgccgttta cccgcgtgga
     7321 aagtctcgat ttcgacgatc acccgttcac cgttcagcag tgggatgagc catgcgccat
     7381 ctgcggatcg acccacagct atcttgatga agtggtgctg gatgacgccg gaaaccgcat
     7441 gtttgtctgc tccgataccg attattgccg ccaacagagc gaggcaaaaa accaatgaat
     7501 caaccgttac tttcggtcaa taacctgacc cacctttacg cgccgggcaa aggctttagc
     7561 gatgtctctt ttgatttatg gccgggggaa gtgctgggca ttgtcgggga gtccggctcc
     7621 ggaaagacca cgctgctgaa atcaatctcc gcgcgcctga cgccgcagca gggggagatt
     7681 cactatgaga gccgttcgct gtacgcaatg agcgaggccg accgccgtcg cctgctgcgc
     7741 accgaatggg gcgtagtgca tcagcatccg ctcgacggcc tgcgccgcca ggtgtcggca
     7801 ggcggcaata tcggcgagcg gctgatggcg accggggcac gtcattacgg cgatattcgt
     7861 gccaccgcgc agaagtggct ggaagaggtg gaaattcccg ccaaccggat cgacgacctg
     7921 ccgaccacct tttccggcgg catgcagcaa cgtttgcaga tcgcccgcaa cctggtgacg
     7981 catccgaagc tggtgtttat ggacgagccg accggcggac tggatgtgtc ggtacaggca
     8041 cgcctgctcg acctgctgcg cggcctggtg gtggagctga acctcgcggt agtgattgtc
     8101 acccacgatt taggcgtcgc ccgcctgctg gcggaccgtt tgctggtgat gaagcagggg
     8161 caagtggtgg agagtgggtt aaccgaccgc gtgctcgacg acccgcacca tccgtataca
     8221 caactgctgg tgtcatcggt tttgcagaat tgagccggtg ccggatgcgg cgtaaacgcc
     8281 ttatccggcc tacaaatgcg ctccccgtag gtcggataag acgcgtcagc gtcgcatccg
     8341 acacccgaac cacgaggcga aaaatgatta acgtacaaaa cgtcagtaaa accttcatcc
     8401 tgcatcagca aaacggcgta cgcctgcccg tcttacaacg tgcctcgctc accgtcaacg
     8461 caggcgaatg cgtggtgctc cacggtcact ccggcagcgg caaatcaacc ctgctgcgct
     8521 cgctgtacgc caactatcta cccgacgaag gtcaaatcca gatcaaacac ggtgacgagt
     8581 gggtagacct ggtcaccgcg ccagcgcgca aagtggtgga aatccgcaaa accacagtcg
     8641 gctgggtgag ccagtttctg cgcgtcatcc cgcgtatctc agcactggaa gtggtgatgc
     8701 agccgctgct cgataccggc gttccgcgtg aagcctgcgc cgctaaagcc gcgcgtcttc
     8761 tcacccgcct gaacgtgccg gaacgcctgt ggcacctggc accatcgacc ttctccggcg
     8821 gcgaacagca gcgcgtcaac atcgcccgcg gctttatcgt cgactacccc attctgctgc
     8881 tcgacgaacc taccgcctcg ctggacgcca aaaacagcgc cgcggtggtg gaactgattc
     8941 acgaagccaa agcccgtggc gcagccatcg tcggcatctt ccatgacgaa gctgtacgta
     9001 atgacgtcgc cgaccgcctg cacccaatgg gagcctcttc atgattatca ataacgttaa
     9061 gctggtgctg gaaaacgagg tggtgcacgg ttcgctggag gtgcaggatg gcgaaatccg
     9121 cgcctttgcc gaaagccaga gccgcctgcc agaagcgatg gacggcgaag gtggctggct
     9181 actacccggc ctgattgagc tgcataccga caacctcgat aaattcttca ccccgcgccc
     9241 gaaagtcgac tggcccgccc attcggcgat gagcagccac gacgcgctga tggtggcaag
     9301 cggcatcacc accgtgctga acgcggtggc gattggcgac gtgcgcgacg gcggcgatca
     9361 gctggagaat ctggagaaga tgatcaacac catcgaagag acgcagaaac gcggcgtcaa
     9421 ccgcgccgag caccgtctgc acctgcgctg cgagctgccg catcacacca cactgccgct
     9481 gtttgaaaaa ctggttcagc gcgaaccggt gacgctggtg tcgctaatgg accactcacc
     9541 gggccagcgc cagttcgcta accgcgagaa gtatcgcgaa tattatcagg gcaaatactc
     9601 cctcaccgat gcgcagatgc agcagtacga agaagagcaa ttggcgctcg ccgcacgctg
     9661 gtcgcagccg aatcgcgaat ccatcgccgc cctgtgccgc gcgcgacaaa ttgcgctcgc
     9721 cagccacgat gacgccacgc acgcccacgt tgccgaatcc caccaacttg gcagcgtgat
     9781 cgccgaattt accaccacgt tcgaagcggc ggaagcctcg cgtaagcatg gcatgaacgt
     9841 gctgatgggt gcgccaaata tcgtgcgtgg cggctcgcac tccggcaatg tggcggccag
     9901 tgaactggcg cagcttggtc tgctggatat cctctcttcc gactactacc ccgccagcct
     9961 gctggatgcg gcgttccgcg tcgccgatga cgagagcaac cgctttacgc taccgcaggc
    10021 ggtgaggctg gtgaccaaaa atccggcgca ggcgctgaat cttcaggatc gcggggtgat
    10081 tggcgagggt aaacgcgctg acctggtgct ggcgcatcgc cagggcaatc acattcatat
    10141 cgaccacgtc tggcgtcagg gtaaaagggt gttctgatga tgggaaaact gatttggtta
    10201 atggggccgt ccggctccgg gaaagacagc ctgctggcgg aactccgcct gcgggaacaa
    10261 acgcagttac tggtggcgca tcgctacatc acgcgcgacg ccagcgccgg aagtgaaaac
    10321 catatcgccc tgagtgagcg ggagtttttt acccgcgcgg ggcaaaacct gctggcctta
    10381 agctggcacg ctaacggtct gtattatggc gtcggcatcg agattgacct ctggctgcac
    10441 gctggattcg acgtagtcgt caacggttca cgcgcgcatt tgccgcaggc acgggcgcgc
    10501 tatcaatcgg cgctgctgcc cgtctgttta caggtttcgc cggagatcct gcgccagcgc
    10561 ctgaaaaacc gtggccgtga aaacaccagt gaaattaacg cccggctggc gcgcgccgcc
    10621 cgctatactc cacaggattg ccatacgctc aacaatgacg gcagcctgcg ccagtcggtc
    10681 gacacactgc tgacgctgat tcatcagaag gagaaacacc atgcctgctt gtgagcttcg
    10741 cccggccacg cagtacgata ccgacgcggt ttacgcgctg atttgtgagc taaaacaggc
    10801 ggagtttgac caccacgcgt ttcgcgtggg ctttaacgcc aacctgcgcg acccgagcat
    10861 gcgctacccg agcatgcgct accatctggc gctgctggat ggcgaagttg tcggcatgat
    10921 cggcctgcat ttgcagtttc atctgcatca tgtcaactgg atcggcgaaa ttcaggaact
    10981 ggtggtgatg ccgcaggcgt gcggtctgaa cgtcagcagt aagttactgg cgtgggcaga
    11041 agaagaagcc cgccaggccg gggccgaaat gaccgaactt tccaccaacg tgaagcgcca
    11101 cgacgcgcac cgtttttacc tgcgtgaagg ctacgagcag agccacttcc gcttcaccaa
    11161 ggcgctgtaa catgagcctg accctcacgc tcaccggcac cggcggcgca cagggcgttc
    11221 cggcatgggg ctgcgagtgc gtagcctgcg ccagagcgcg gcgctcaccg cagtatcgcc
    11281 gccagccatg cagcggcgta gtgaagttta acgacgcaat caccctgatc gacgccgggc
    11341 tgcacgatct cgccgatcgc tggccgcccg gatcgttcca gcagtttttg ctgacgcact
    11401 accatatgga tcacgtgcag gggctattcc cgctgcgctg gggcgtgggc gatccgatcc
    11461 cggtttacgg cccgccggat gaacagggct gcgacgatct gtttaaacat ccgggcctgc
    11521 ttgatttcag ccacacggtg gaaccgtttg tggtgtttga tttgcagggg ttacaggtca
    11581 cgcccctgcc gctcaaccac tcaaaactga ccttcggtta tctgctggaa acggcacaca
    11641 gccgggtggc gtggctgtct gacaccgcag gcttgccgga aaaaacgctg aaatttttac
    11701 gcaataatca gccgcaggta atggtgattg attgcagcca cccgccgcgc gaggatgcgc
    11761 cacgtagtca ctgtgattta aacaccgtgc tggcgctgaa tcaggttatc cgctcgccac
    11821 gggtgattct gacccatatc agccaccagt ttgatgcgtg gctgatggaa aacgcactgc
    11881 cgtcggggtt tgaggcggga tttgatggga tggagattgg ggtggcgtga tgagagggaa
    11941 tgtgcgcgct ggccccctca ccctaaccct ctccccagag gggcgagggg accgattgtg
    12001 ctcgatattg aatattgcgc tggttttctc cctctcccca ttggggtgag gggcgatgcc
    12061 tgctccatac ccaacctcat cgcccatact catcttccat tctccgctct tcatcttcta
    12121 gctgccgacg ctcctgatca agctggcgct ggcgatcgtc cagctgcctg cggcgatctt
    12181 caaactggcg gcggcggtcg tcatattgtc tgcgccgatc gtcgctcact tcacgctgcc
    12241 agccgtcgtc gcgcgaatct tcatagtctc gcccacggtc agggttataa gcgtcgttaa
    12301 tcgcctgctg aatattgcca atggtgtcgt cgataatatc ggcctgggcc ggaacggtgg
    12361 acagcgtgag cagggtgaat aaaagaaata gcggaaagcg tttcattagc caacctcaaa
    12421 aagaaactct atccacatta atcattactc atccatgcaa gtagtggatg aatctcaatt
    12481 tctccgctgc tctattgccg taatcgcctc cacgcgttgt tgatgacgac cgccttcgta
    12541 ctgtgcgccc agccacgcat ccacaatcat ttttgccagt tcgaggccaa ccactcgtga
    12601 accaaaagcc agtgcaactt cactggcata atgcggataa tcagtgcgct ctgacgacct
    12661 ggtgtcttta tcaatcacct cactcattga ccatgaataa aaggctggca cagcaacgca
    12721 gaatcctgcg gtgccagcga catgaccgcc cattgctccg gtacgttttt taaaaagaaa
    12781 caccggttgc attgataaat tgtgctcagt atttgccgaa gtacaatata tataataaat
    12841 atataaaaca cctcatatta tttacagaga taataaattc gcgtaatttt ctctcttcag
    12901 cacaaatgcc taataaaatg gatcgttcag ctcacacttc cacttattaa agtcgcaaat
    12961 tcccaattat gataacccca gctttaaatg acgtattttg tatggattac atatttacga
    13021 cataaatgat aaatggagat ttatatgaat aactctcggt tattccgttt gagcagaatt
    13081 gttattgcgt taactgccgc cagcggcatg atggtaaata ccgctaacgc gaaagaggaa
    13141 gcgaaagccg ccactcaata tacccaacag gttaatcaga attacgccaa atcattaccg
    13201 tttagcgatc gtcaggattt tgacgacgct cagcgtggat ttatcgctcc gctgctggat
    13261 gaaggtattc tgcgcgatgc taatggcaaa ccatatta
//
LOCUS       DAJTSL010000109        13292 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTSL010000109 DAJTSL010000000
VERSION     DAJTSL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.109"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..1862)
                     /gene="speC"
                     /locus_tag="OO433_002842"
                     /pseudo
     CDS             complement(1..1862)
                     /gene="speC"
                     /locus_tag="OO433_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            2260..2958
                     /locus_tag="OO433_002843"
     CDS             2260..2958
                     /locus_tag="OO433_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6051253.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            3064..3139
                     /locus_tag="OO433_002844"
     tRNA            3064..3139
                     /locus_tag="OO433_002844"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3097..3099,aa:Phe,seq:gaa)
     gene            complement(3288..3824)
                     /gene="yghD"
                     /locus_tag="OO433_002845"
     CDS             complement(3288..3824)
                     /gene="yghD"
                     /locus_tag="OO433_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HCR6051254.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETMLQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(3826..5004)
                     /gene="gspL"
                     /locus_tag="OO433_002846"
     CDS             complement(3826..5004)
                     /gene="gspL"
                     /locus_tag="OO433_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCR6051255.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGVMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5001..5978)
                     /gene="gspK"
                     /locus_tag="OO433_002847"
     CDS             complement(5001..5978)
                     /gene="gspK"
                     /locus_tag="OO433_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCR6051256.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5981..6580)
                     /gene="gspJ"
                     /locus_tag="OO433_002848"
     CDS             complement(5981..6580)
                     /gene="gspJ"
                     /locus_tag="OO433_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCR6051257.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6577..6948)
                     /gene="gspI"
                     /locus_tag="OO433_002849"
     CDS             complement(6577..6948)
                     /gene="gspI"
                     /locus_tag="OO433_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCR6051258.1"
                     /translation="MKRGFTLLEVMLVLAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(7008..7508)
                     /gene="gspH"
                     /locus_tag="OO433_002850"
     CDS             complement(7008..7508)
                     /gene="gspH"
                     /locus_tag="OO433_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCR6051259.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNAC"
     gene            complement(7512..7967)
                     /gene="gspG"
                     /locus_tag="OO433_002851"
     CDS             complement(7512..7967)
                     /gene="gspG"
                     /locus_tag="OO433_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCR6051260.1"
                     /translation="MNSLSRTHKPRAGFTLLEVMVVIVILGVLARTVVPNLLGNKEKA
                     NRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7984..9207)
                     /gene="gspF"
                     /locus_tag="OO433_002852"
     CDS             complement(7984..9207)
                     /gene="gspF"
                     /locus_tag="OO433_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCR6051261.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAM
                     SDALQASGVYWLAGLLALLVLGQWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYIIASGEQRGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9207..10699)
                     /gene="gspE"
                     /locus_tag="OO433_002853"
                     /pseudo
     CDS             complement(9207..10699)
                     /gene="gspE"
                     /locus_tag="OO433_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            complement(10699..12759)
                     /gene="gspD"
                     /locus_tag="OO433_002854"
                     /pseudo
     CDS             complement(10699..12759)
                     /gene="gspD"
                     /locus_tag="OO433_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(12789..>13292)
                     /gene="gspC"
                     /locus_tag="OO433_002855"
     CDS             complement(12789..>13292)
                     /gene="gspC"
                     /locus_tag="OO433_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HCR6051262.1"
                     /translation="QGETLGSHNAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAAT
                     NKKAVSDEAKQAVAEPAVSAPVEIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAV
                     KPGADRSLFDASGFKEGDIAIALNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARH
                     DISIALR"
BASE COUNT         2969 a         3840 c         3431 g         3052 t
ORIGIN      
        1 tatgcgcatc ctgggggttc atcaccactg acgggaaact ctgctggcgg aacatcgctt
       61 tttgtaggtc tttgacgtcg aaactgacat acagatcgtg catctcctga cacaactggc
      121 gcaaagtgta gtcgcgatag cgcaccggat atttgttata aacgctcggc aacacctcag
      181 ccagcggcga gtcatcctca atatgctgtt caaactgcgc caacatctct accagttgtg
      241 ccagcttctc gtggctttcc gccggagtta ataagaacag aatggagttg agatcgcact
      301 tctccggcac aatgccgttc tcacgcagat agtgcgccag aatcgtcgcc ggaacgccaa
      361 agtcgctata ttcgccggtt tcggcattga tacctggtgt agtgagtaac agcttgcacg
      421 gatcaacaaa atactgatcc gcggcatatc cttcaaagcc gtgccacttc gcccccggct
      481 caaaactgaa aaaacggcgg tcgctggcta acactgatgt cggataatcc tgccacaatt
      541 tgccatcaac aacgggcggg ataaacgggc ggaacagctt acagcgcgca agaatagcct
      601 tgcgcgactc aatacccaac tcaacgcact cagcccacag ccgacgccca ctctcccctt
      661 catgaatttt ggcgttaaca tccagtgcag caaacagcgg atagaaaggg ctggtagaag
      721 catggagcat aaaggcgtta ttcaaccgct tatgcgggca aaaacgcgcc tgtccgcgga
      781 tatggttatc ttttttatgg atctgcgacg tctgtgagaa tcccgcctgc tgtttgtgca
      841 ccgactgagt cacaaagatc tccggatcgt tttcgttaag ttctaatagc agcggcgagc
      901 tatccgccat catcgggata aactgttcgt aaccgaccca cgcggaatca aacagaatgt
      961 aatcacacag atgcccaacg gtatcgatca cctgacgggc gttatagaca gttccgtcat
     1021 aggttcccag ctgaataatc gccaggcgaa acgggcgcgg cagctcggct ttttctggcg
     1081 caacgtcgcg aatttgctgg cgcagatact cttcattaaa acagtgcgca tcaataccgc
     1141 caatgaaacc aaacgggttg cgtgaagctt ccagatagac cggcgtcgcc cccgcctgaa
     1201 tcagcgcgcc gtgatgattc gacttatggt tgttacggtc gaagagcacc agatcgccac
     1261 gcgttaacag cgcattcgtc accactttat tcgctgccga tgtgccgttc agcacaaaat
     1321 aggttttatc ggcatgaaag actttggctg cgaatttctg cgcatctttc gccaataagc
     1381 agatcgccca attttacgtc agcgttacac atatcggcgc gaaagacgtt ctcaccaaag
     1441 aaatcgtaaa aatggcgtcc ggcaggatgc tttttaaaaa acgcaccatg ttgatgtcca
     1501 gggcaagcaa aggtgctgtt gcccatctca acgtactgcg tcagcgtgtc ataaaacggt
     1561 ggcagcaaat tctcttcata ctgacaggct gcggattcca gctccagcca ctgctgctcg
     1621 ttgccgttga ttaccgccgt aacgcccgca ggtaattcaa cagcatgttc ggaatacaaa
     1681 aacaccggta gatgaaaacc ggtgcgctta agcaacgcaa gaatgccact gcgactatcc
     1741 gcagcggtaa tgacgactgc cacgacgtcc gtaaaatcag tatctcccaa cgccaccacg
     1801 cgacgatgag aagaaagtcg ggataccagt tcactactgg cggcaatatt cattgatttc
     1861 ataagcgcaa acccgtttcg gggaagtaag aataccggac aaggtggaaa accctgcccc
     1921 atgagatatg ggtcaaactg gtcaccagct ccgaccgcca gacatcagta aaagcagaaa
     1981 cgctctgatt ttactgttgt cctgcagtga gcgtgcgtta acttcaccgc atgagcagta
     2041 acatagaaag ggaaacgttt cgcgcgaaac ggcgataagc gagagaatgt aaggaggtgg
     2101 cgtgcatcgg gcaaactccg tacagagagg agaaaattcg cgcaatcatg gcacctttcg
     2161 ctaaagcgtg caagccagga ctttgcgacc agacaagcca tcaacaaggc aaaaagttca
     2221 taataagaaa atcaaacaat acagttaaca ggaactcttg tggtcatcgg cccttttatc
     2281 aacgctagtg cagtcttact gggtggcgtt ctcggcgcac tgctcagcca acgcttaccg
     2341 gaacgtatcc gcatctccat gacatctatt tttggtctgg catcgctggg gattggtatt
     2401 ttactggttg tgaaatgcgc caaccttcca gcgatggttt tagctacgct acttggcgct
     2461 ctaattggcg aaatttgttt gctggagaaa ggtgtcaata cagcagtcgc caaagcacaa
     2521 aatctgtttc gtcactcacg taagaagcca acgcatgaat cttttattca gaattatgtc
     2581 gcgattattg tcctgttttg cgccagcggc accgggatct tcggggcgat gaacgaaggg
     2641 atgaccggcg atccgagtat tttaatcgcc aagtcatttc ttgatttctt tacggcgatg
     2701 atcttcgcct gctcgctggg tattgcggtg tcggtaatta gtatcccatt actgatcatc
     2761 cagttaacgc tggcgtgggc tgccgcgctg atattaccgc tgaccacacc gtcgatgatg
     2821 gcagacttca gcgccgtagg cggtttattg ctgctggcaa ccggattacg catctgtggc
     2881 attaaaatgt tcccggtggt caacatgctt ccagcactcc ttctggcaat gccgctttcc
     2941 gccgcctggt ttgcctgaca atgcgtgcaa tagcggcaaa gtgatgatag attgtgcagt
     3001 ctgcagtaaa ttgaagaaat ttgattgacg agacgaggcg aatcaggttt aatgcgcccc
     3061 gttgcccgga tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg
     3121 attccgagtc cgggcaccac taattcttaa gaacccgccc acaaggcggg tttttgcttt
     3181 tggatctgac aataaccttc acgaaaaaag tttgcttata aagtctgggg gaattactct
     3241 cgccacgtta acgagagtaa ttttattgat actaatctcc tgatacttta cccccgtcca
     3301 aactccagcc gctggacatt caccatccca gtcttctcag cagcactggc atcaatttgt
     3361 gtcacccgca gcgcatactt ttcatccagc gcgtttaacc agttcagcag gtcattaaac
     3421 accaccggat ctatccagac ctgaatattc tccccacgct cggctatccg cttgatgact
     3481 accgagtgcg cggaagcact gtcactgata acccgcgata cctgcgcagg cgttgttgtg
     3541 gcggattttc gcgccgcaat aatatccggc gcggcgctcc tcagtctggt gttcatcgcc
     3601 accagttgct gcaacattgt ctcctgttgt actatccgtt cgctcaacgg ctgccagatg
     3661 aggacgtaat atccgacgct aaacaggaac accaccgctg ccagtaacat gcccttttca
     3721 cgcggagaac gccccgccag gtgttgtgcc agccagtgtt cgccacggct taactggcgt
     3781 tcacgccatt gctgaaaata gtgaataaat ttatcgcgta acatgttatt tcctccgcaa
     3841 cgttacgccg ccggaaaccg catcaccctc tttctgtaac gcgtcctgtt gcacaacata
     3901 atcggttgcc agtgcactac gcagtttgtc gaaactggca aagttcgcgg cccgtagctg
     3961 gaggtgaagt atctggcgtt tttgatcaaa ggtaaaacca cgcatttcga tgtcggaaag
     4021 tgacgctgat ttcagggtgc tggctatcgc tgacaactcg gcgagcagcc gggtatcgtc
     4081 ggcctgtggg cgatattttt tcagcgccat cgtcacctga gagcgtaaat tcacaatccg
     4141 cttctgctcc gggaacaacg ttaagaactg ttcctccgcc tgggtgcggc tttgcgccac
     4201 ctgttcgctg acgctccata acgtcacgcc ccgctccact gccagcgcaa ctagaatcag
     4261 caatatcggc agaatcatca cccgccagcg cgcccactgt tttcggtagc tgacgcgagg
     4321 ctgccacggc cctgtcagca ggttcccttc cggttcgcca taagtggtaa cggcgggcag
     4381 agctgtaacg gtcaggcgtt cagcgtctgc gccagcccat gctgatagct tttccggtgc
     4441 aatgccgact acggttagcg aaagcggtaa atcctgctca ttgagctggg cgcggaacat
     4501 gaccggagcc agcgcccgcc cggcgctcca tccccggcat tcatcaatgc ggcagataac
     4561 ccgttgcgca tcgccagcca taaccccaca aggaatggac atccagtctg gcgcgacggt
     4621 agcgcgggtg atgccgtttt cctgcaacca ctgcgcaatg ttgcgcatat gctgttggtg
     4681 aatcactgct acggttgcca gttgctggtc gatattcaac ggggcgaaat gcagttcatc
     4741 gatatcctgg ttcagctctt cttccagtaa ggcgggcaga atggtcggta tctgcttacg
     4801 ggcgacatca ggcagttcaa cctgccagac gctgatccat tcgccgggaa tgtagagtcg
     4861 aatcgcatcg gtttgcagcc attgctggag ctgttcgtta gcaacgtcag accagatacc
     4921 gtgctccaca tcgggtgtac ggcgctgcca atggatggga tcagcggcgc aaagcgggaa
     4981 aaaaatctca aggatggaac tcactcactt tctcctgtct gatgccagag aaccgaaaag
     5041 tgttgtgggc ccatgcggac aattaacgag ttcatcgtca gttcaatctc attcacggtg
     5101 atatctgaac gcagccagaa gtaattgctg tccacgctca ggacggtttt tagctgtttt
     5161 ttagtacgct catcgacgtc agcaagtagc ggctgtgcaa gaaactgatc gacatcttcc
     5221 cagcccttcg ccggacgttg ttgtaataac gcccgcgcct gaacggggct taaccacggg
     5281 tcaaacagcg cctcaagaat cacactttgc gtgacgtcta aagtattgat gttgatttgc
     5341 tggcggatca tcggcagcgc acagaccagc ggtttcagtt tttgataaag cccggcgtcc
     5401 attccctgta ccacgcgcat ctcactgata tcagccagcg gttgattggc ggcgtagaac
     5461 ggcaccgaac gggcgagata ctcgctgtct tcacggccca gacgtgtctg cacgctgcgg
     5521 tcctcgtcaa taaactccca caggctttcg gctatcagtt cggcccgata agcaggcaca
     5581 tccaggcgcg agatcagggc aatcaactgt tgtaccgcaa gcggacgcga cgccgttgtc
     5641 ggctgagcaa gggcattcag gttaaagcaa gcctgtgcgt cacgcagggt gaccgcaatt
     5701 tgcccctgcg gcagagggaa gaaacgaggg ccggatgccc acggctgtgc cagatgcacg
     5761 cgcttttcat ttttcaggct cagactgagc gcgctcagcg ccaggctttc cgcactggcg
     5821 ctgtaccaca gcgcctgctg gtactcctgc tggctgcgcg ttcgcccaag ttgttgctgc
     5881 attcgcccgg aaagcgtgat ggttaccagc atcataaccg ccagcaatac cagcaccacg
     5941 accagtgcca ttccgcgttt tggtggtgag gtgatcatga ttattgcggc ccgcgtaaca
     6001 accagatgcg ttcaatctcg ccccattgcg gcgaatgcag ggtcatgcgt accgccacag
     6061 ggatcgcctg tactgacgac cagctctcct gccagcgcgt gccgtcgtag aactgcaaac
     6121 ggagcgaatc ggccggaatc aacttttgca ttgtcggctt cacgctgcct gcggcatcgg
     6181 tcagcggcca ggctaaccgt tcgagataac cgtcatgaat gcggtaaccg acggtgagca
     6241 gattgctgcg cggcagacgc atcaacgggt taaccacacc gccgcgcaca aaacgcattc
     6301 cttcactctc agacgccagc acgccagcgc cagccagtaa agctggttca cgctggccct
     6361 gatcgcctct taccggacgc ggcatcattt gggtcagatc gtgggtcaga aaactcatcg
     6421 tttgctgcat gaggtttagt ttttgatcgt gatcggcgac ggcgctattc acgcgcgtga
     6481 cgccgttagt cacctgctgc gccatcagtg ccagcgaggc aaaaatggca attgccacca
     6541 acatttccag taacgtgaaa ccagcgcgag tccttctcac tgttggcctc ccacggcgct
     6601 aaaccaggcg cgtcgtgact gaatcaccga cgaaaagtct tcgtgacggc tgacttcaat
     6661 atccaccgcc tggagcagcg cgttgcctgt attcaatggg attgttcgcc agtaccagcg
     6721 gcttcctgcc atatcgctct cgccctgatg cctgaccgcc cgctgctcac gggtcatcag
     6781 gtagagcagc gcagtctggt tttcagctac ccagcccgct accgtttttt cctcaagaac
     6841 gtgctgatta ctcagcgcac cgctggcaat ctgtaacacc gccgtggcag ccagcgcaaa
     6901 aatcgccagc acgagcatca cttccagcaa ggtaaatccg cgcttcatgg catcctctca
     6961 tcacattgat tgagggataa cgcgccatcg tgtgccagct ttaccgctca acatgcgttt
     7021 tgcgccgccg agtagaagcg cagcgtaaac ggcgtggcgg gttcaaaagg cgaaaaacgg
     7081 atctgcggcg tcttcttttt cgcctctttt tgcaactcca gttcaatatc gtgcagcgtc
     7141 aggcgacgac gttgcagctc cagcgcatat tccttctgcc agatatcact tccgggttgt
     7201 aacagcatct gcacctgttt cggcaccgta acctgtgccg ataaccgggt cgcagaaacg
     7261 ggcaaccact gcccctgacg acgctgcata aactgatagc caggcgggtc gattagcaca
     7321 ccgagtgtct gtccttcaat cactgccctg tccttaaact gcgcaaagcg agtcagaaaa
     7381 tcctgtgccg cttttttcgc gggcggttca gaatcggtcg caaacgtctg taccacgccc
     7441 gcactggcaa ggccgataag gaaaatcacc agcatgattt ccagaagtgt gaatccgcgt
     7501 tcaggcactg cttactgaaa ctcctgcaaa ttccagttac cgatatctgc gccagcgcct
     7561 tccccgtttt cctgaccatc cgcccccagg gtataaacat caaacagtcc tttttcaccc
     7621 gggctgagat actgataatc attgccccac ggatcctttg gcagtcgttt gatgtatcca
     7681 ccagtacggt agttgcggga gtccgccata ttggctggct gctggatcag cgcttccagc
     7741 ccctgttctg tggtcggata acgcccgtta tccagtcggt acatatctag tgcgttttcc
     7801 agcgccacga tatcgctgat ggctttttgc cgattggctt tctctttgtt gcccaacagg
     7861 ttaggcacca ccgtccttgc caggacgcca agaataacaa tcaccaccat cacttccagc
     7921 agggtaaaac ctgcccgtgg tttgtgtgtg cgggataacg aattcatgtg ataactccgt
     7981 aaattacatt ccaaccatat tattcagttg cagcatcggc tcgaggatgg cgatgacaat
     8041 aaacagcacc acgcccgcca tcatcaccac cagcgcaggc tcaaacagcc ccaacgccag
     8101 ccccacctgg gtatcaaatt cccgttcctg gttaacagcg gcctgctcaa gcatggtttc
     8161 cagttcgcct ctctgttcgc cggaagcgat aatgtacagc atcatcggcg ggaacaggcg
     8221 caactccgcc agcgcggcgc gcagactgct tccttcgcgg acgcgatctg ccgccagcag
     8281 cagttgttgt tcgacatagc gatttgccga cacggcggca gcggtctgaa tgccttccag
     8341 tagcggaaca ctgctggcgg tgaggatgct taatgtgcgg gaaaaacgcg ccgtattcag
     8401 cccgcgcgca acacggcccg tcacgggcag acgcagcaag gttttatccc agcgcaggcg
     8461 catagtagga tttttaagta gccattgccc cagcaccaga agcgccagca atcccgccag
     8521 ccagtaaacg ccgctggcct gtaacgcatc actcatagcg ataagcgcac gggtggagga
     8581 gggtagcgcg tgtccgagat gatcaaactg ttcgatgatt ttcggcacca ctgccgtcag
     8641 caaaatagtg accacgcccg ttgccaccac cagcataacc agcggataga gcatggcctg
     8701 caatagacgt gatttcaggc gctgtcgctg ttcggtgtaa tctgccaggc gattgagcac
     8761 cacgtcgaga tgcccggatt tttctccggc agcgaccatc gaacaaaaca gggaatcaaa
     8821 gacgcgggga tgttcgcgca ggctgtccga cagggtataa ccttcctgaa tccggctgcg
     8881 cagcgccatt ccgaggctct tcacatgcag tttttcactt tgctcactga ccgcctgcaa
     8941 gcaggtttcc aacggcattg ctgcctgcac cagcgttgcc agttggcgcg tgaacagcgc
     9001 cagatctgcc gccgccacgc gacgatgtgc gtgccgccga cgctgcaaca ttccccctgc
     9061 cgatgcattc atcctggctt caatgtgcac ggggataagc tctttaccgc gcaacaactg
     9121 gcgggcatga cgcgcggaat ccgcctcaat catgcctttg gttttgcgac cattacgctc
     9181 cagcgcctga tagtaaaaca gtgccattac gcctccatgg ttacccgcag aacttcatcg
     9241 agagaggttt ctccggcgag cactttctca atgccgttgc tgcggatacc cgcagagtgt
     9301 tgccggacat aacgttccag ctccagctcc ccggcctgac ggtggatcaa atcacgcaat
     9361 gtggcatcca ccacgtcagc tcatggatgg cagtccgtcc gcggaaacct ttgtgattac
     9421 aggcggggca gccctgcgga tggtacagag tgacggttcg cgcatcggta atgcccagca
     9481 ggcgtttttc ttcgtcagtg gcaggcgcgg cctgacggca gtcgggacac agcgtgcgga
     9541 ccagtcgctg cgccatcacg cccgtcagac tggaagagag caggaaaggc tccacgccca
     9601 tatcctgcaa acgcgtgatc gcccccaccg ccgtgttggt atgcagcgtg gaaaggacca
     9661 ggtgtccggt aagcgatgcc tgaacagcga tttctgcggt ttcggtatcg cggatttcac
     9721 cgaccatcac cacatccggg tcctgacgca aaatcgcgcg caggccacgg gcgaaggtca
     9781 tgccgacgcg ggtgttaacc tgtgtctgac cgatcccttc aatcatgtat tcgatggggt
     9841 cttcaaccgt gagaatgtta cgcgaatggt tgttcagctc ctgcaatccg gcgtacagcg
     9901 tggtgctttt gccggaaccc gtcggccccg tcaccagaaa gatgccgtgc ggtttgtgta
     9961 acagctggcg caactgcacg gtcagttgct ggctaagccc cagacgctcc agcgtcaggc
    10021 gggcctggtt tttgtccagc agtcgcagca ccacgcgctc gccccaggca gaaggcatgg
    10081 tggagacacg cacgtcaatc gcccggccgc ccagcagcag cgcaatacgt ccatcctgcg
    10141 gtacgcgctt ttcggcgata tccagccgtg ccatcacctt gatacgcgat accagcagcg
    10201 aggccagttt gcgccccggg cgcagcattt catgtaatgt gccgtcaacg cgaaaacgga
    10261 tcaccagact cttttcaaac gtctcgatgt ggatatccga agcgccttct ttaatcgcct
    10321 ctgccagcat ggcgttgatc agtttgatga tcggcgcatc gtcgtcactt tccagcagat
    10381 cttccgtttc tggcagttct tccgcgagag taaaaaagtc ctcagcagaa ccgagatctt
    10441 ccatcagctg gcgagcctcg gaagagtccc gctgccagac cgcattcagc cgctgttcaa
    10501 attcggcctc gtcgatttgc cgcagcgtaa agggcgcgtt cagcccccgc tgcagctcct
    10561 gcaaaacaga gagcgatagc ggatgaacgt ggaggatctc cagcgacgct tcgcaccatg
    10621 ccaccaggct aaaccgacgg ctgaaactgt agggcagacg cacggtcttg gcggtggttt
    10681 cctgtgctac aggcaccatt aacgcgttct cccggcattg aggaacgcgc gaacttccgg
    10741 cggtaaggcc tggttttgcg ctggcagtac cggctgtgcg gtgtgcggca tcaggcttaa
    10801 accttgctca tcacgataga tctgttcggc gcgcatatag ttatatttgc gctgcgacac
    10861 gccgtctgcc gccataccgt catgcagaat ggtcgggcgg ataaacacca tcaggttacg
    10921 tttttctttt ttatccgccg tcgatttaaa caggttacca atcaatggga tatcgcccag
    10981 cagaggcact ttcgccacgc tttctcccgc ctgatcgtcc atcagaccac caagcacaat
    11041 cagctcacca tcgttagcca gcacggtggt tttcagtttg cgctcaccaa acaccacgtc
    11101 gaggctggtc tgtccttcca ccttcgagac ttcctgctca atcaccatct gtaccgcgtt
    11161 accttcgtta atctgcggcg tgactttcag catgatgccg acttttttcc tttctactgt
    11221 attgaaagga ttgctgttat tggagccaac ggtagagccg gttaataccg gaacgtccta
    11281 gcccaccatg aagaaggctt cctggttgtc cagcgtagtg atgctcggcg tggagagaac
    11341 gttcgagctg gagtcgtttt taaccgcctg caccagtgcc atccagtcgc ctttcaccac
    11401 gccaaccgcc gtaccgctaa atccagaaag aagctgggcg agtgtggaga gatcgccgtt
    11461 ggtatccgga ttaatggtgg tagcgccgtt ttcactgatc accgtcgagc ctttctgcgg
    11521 tttcgcctga gaaatggctg cccccagcgt accgataggg atctgcgtac cattagcaaa
    11581 ctgcattaat ccggcatctt tcgatgccca ctgcacgccg aagttgatat tgctaccttc
    11641 ggcaacttcc acgatcaacg cctcgacgtg cacctgagca cggcgaatat ccagttgttc
    11701 aattacgctt tgcagcgact gcatgatgtc ctgcggcgcg gttacaatca gggcattact
    11761 gtgtttgctg gcggcgatgg atacaacctc acgcccgcta ccgaccgtgc cttccgcctc
    11821 ttctttagcc gccgtgagcg tgccgctgac ctgtttcagc acatcgacca gatcttcggc
    11881 tttgctgtat ttgagataga aaacctggct gttgccgctg cgttccattt ctgagtccag
    11941 ccgacggatc aggcggcgca ttttgtcccg cgtggccggg tcaccactga caatcacact
    12001 gttggtgcgt tcgtcggcga caatttgaga tttcagcgtc gcaggctggt tctcgccgct
    12061 gtttttcgtc aggctttcca gcacgcgggc gatttccgaa gccgaggcgt tatccagcgg
    12121 gatcacctct tcggtgcgat tacccgcgtg atccacgcgc tggatcactt ccgtcaggcg
    12181 ctccacaacg gaggcgcgtc cggtgagcat aatcacgttg gagggatcgt aattaacaac
    12241 gttgcctgag cctgcgctgt cgatcatctg gcgcagaatc ggtgccagct cgcgtaccga
    12301 cacattacgt accggcacta ctttggtgac catttcatcg cccgcgtagt tgtcgctgcc
    12361 ttcaccgacc agcggcagcg gctcgacttt cgcagcgctg gatttcacca ccttcagcac
    12421 gtcgttttcc atcggtacga cggcataccc ctgtgcttcc agcaggttaa ggaatagctg
    12481 gtaatactgg cgttcattga gcggggtcat ggtgcgaata ctcactttcc cctgtacgcc
    12541 cggccccata atgatggttt tattaaggtt agcgccgacg gtttcgatga acgatttcag
    12601 gtcggtatct ttaaaattag cggtgaaagt ggcttcttcc gcccagaccg gtgaactgca
    12661 taatgccgct gccagcacca gcggcagtaa acgctttttt gttttgaggc cagttgtctt
    12721 cttacgccag accgacaagg taatatcacg ccaaaacacg atgaatgatt ctcctggatt
    12781 aaatgcggtt agcgcagcgc gatggaaatg tcgtggcgcg cacccttgcg taaaaccgta
    12841 agttgaatgg aatccattga aggtaactgc cgcatcagag caatcattgc tcgtggatca
    12901 gtgaaatcct gctgatttag cgcaatggcg atatcgcctt ccttgaaacc gctggcatcg
    12961 aacagagaac gatctgcccc cggcttcact gcataaccga caattccctc cttacgcaca
    13021 ggcgtaagct ggatatagtt aaaaattttc tgcggatctt tcgccagtgc ctgacgcacg
    13081 gcagtcggta tctcaactgg cgcactgaca gcaggttcag caacagcttg ctttgcttcg
    13141 tcactgacag cttttttgtt ggtcgcggca acggtggaac gctcctcttc agccaggctc
    13201 aggcgctcta tttttccctg atagcgcagc atcacatggt cgcgattgat ttcctcaatc
    13261 accgcgttgt gcgagccaag cgtttcaccc tg
//
LOCUS       DAJTSL010000110        13163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTSL010000110 DAJTSL010000000
VERSION     DAJTSL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.110"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(84..1175)
                     /gene="ychF"
                     /locus_tag="OO433_002856"
     CDS             complement(84..1175)
                     /gene="ychF"
                     /locus_tag="OO433_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6051263.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1292..1876)
                     /gene="pth"
                     /locus_tag="OO433_002857"
     CDS             complement(1292..1876)
                     /gene="pth"
                     /locus_tag="OO433_002857"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6051264.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2154..2432
                     /gene="ychH"
                     /locus_tag="OO433_002858"
     CDS             2154..2432
                     /gene="ychH"
                     /locus_tag="OO433_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6051265.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2487..4166)
                     /gene="dauA"
                     /locus_tag="OO433_002859"
     CDS             complement(2487..4166)
                     /gene="dauA"
                     /locus_tag="OO433_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6051266.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVIIYPVSQQFGL
                     AGLLVAALLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPISGRLAFFPNRRAAMADL"
     gene            complement(4291..5238)
                     /gene="prs"
                     /locus_tag="OO433_002860"
     CDS             complement(4291..5238)
                     /gene="prs"
                     /locus_tag="OO433_002860"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6051267.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5389..6240)
                     /gene="ispE"
                     /locus_tag="OO433_002861"
     CDS             complement(5389..6240)
                     /gene="ispE"
                     /locus_tag="OO433_002861"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6051268.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6240..6863)
                     /gene="lolB"
                     /locus_tag="OO433_002862"
     CDS             complement(6240..6863)
                     /gene="lolB"
                     /locus_tag="OO433_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6051269.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTHPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7077..8333
                     /gene="hemA"
                     /locus_tag="OO433_002863"
     CDS             7077..8333
                     /gene="hemA"
                     /locus_tag="OO433_002863"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6051270.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8375..9457
                     /gene="prfA"
                     /locus_tag="OO433_002864"
     CDS             8375..9457
                     /gene="prfA"
                     /locus_tag="OO433_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6051271.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELRKAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9457..10290
                     /gene="prmC"
                     /locus_tag="OO433_002865"
     CDS             9457..10290
                     /gene="prmC"
                     /locus_tag="OO433_002865"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6051272.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10287..10679
                     /locus_tag="OO433_002866"
     CDS             10287..10679
                     /locus_tag="OO433_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6051273.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10683..11492
                     /locus_tag="OO433_002867"
     CDS             10683..11492
                     /locus_tag="OO433_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6051274.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11528..12382
                     /gene="kdsA"
                     /locus_tag="OO433_002868"
     CDS             11528..12382
                     /gene="kdsA"
                     /locus_tag="OO433_002868"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6051275.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12531..12638)
                     /locus_tag="OO433_002869"
     CDS             complement(12531..12638)
                     /locus_tag="OO433_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6051276.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13066..>13163)
                     /locus_tag="OO433_002870"
     CDS             complement(13066..>13163)
                     /locus_tag="OO433_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6051277.1"
                     /translation="KFAMIFWHDLAAPILAGIITAAIVGWWHNRK"
BASE COUNT         3260 a         3360 c         3406 g         3137 t
ORIGIN      
        1 aaaaatccac gcaattgcgt ggattttata tacttttgcg ctcttcatga gatttagcga
       61 aacctcatga gacaataaat taattagacg ttgaaaagga agttcatcac atcgccatct
      121 ttcacgatgt aatctttacc ttctgcacgc attttgcctg cttctttcgc gccttgttca
      181 cctttgtaag tgatgaaatc ttcaaacgag atggtttgtg cacggataaa gcctttttca
      241 aaatcagtat ggattttgcc cgctgcctgc ggcgcggttg ctccaaccgg aatggtccat
      301 gcacgcactt ctttcacccc agcggtgaag taagtttgca ggttcagcag cttataaccg
      361 gcacggatca cacggttcag gcccggctct tccagcccaa gctcctgcat aaactcgtca
      421 cgttcttcgt cgtccagttc ggcaatgtct gcttcaacag cagcacaaac cggaaccaca
      481 acagaacctt ctttcgccgc gatttcacgc acctggtcaa gatatgggtt gttttcaaaa
      541 ccgtcttcgt tgacgttggc gatgtacatt gttggtttca gcgtcaggaa gctcaggtaa
      601 cgaatagccg ctttctcttc agcgcttaaa tccagcgcgc gcagcatacc tgcattttcc
      661 aactggggca ggcatttttc caggaccgcc agctcagctt tcgcgtcttt atcgccacct
      721 ttggctttct tctgtacgcg atgaatcgca cgttcgcagg tgtcgaggtc tgccagcgcc
      781 agttcggtgt tgataacttc aatatcgtca gccgggttaa ctttgccgga aacgtgaatg
      841 atgttgtcat tttcaaagca gcgaacaacg tgaccgatcg cttcggtttc acggatgttg
      901 gtcaggaact ggttacccag accttcgcct ttcgatgcgc cttttaccag accggcgata
      961 tcgacaaatt ccatggtcgt gggaagcgta cgctgcggtt ttacgatttc agccagttga
     1021 tccaggcgag gatcaggcat tggtacgacg cctgtgttcg gctcaatggt gcagaatgga
     1081 aagttggccg cttcaatacc ggctttggtc agcgcgttga acagggtaga tttcccgacg
     1141 ttgggcaaac cgacgatacc gcatttgaat cccatgattt aaatcacctt aatatcttaa
     1201 taatcaacct gttattgcta acagattgca gaaatggaaa taactttgcc tattatacac
     1261 ggcactcggc aaaaatgccg cagacaacga cttattgcgc tttaaaggcg tgcaatcggt
     1321 tcgttgcttt ggtcaagcca tctgtaaacc acatttcagt acaacgcgcc gcttcgtcaa
     1381 tggcttcatc aattaacttc tgttcactaa caggcggttt gcctaacaca aaaccgacaa
     1441 ctttattttt atcgcccgga tgaccgattc cgatgcgtaa acggtgaaag ttagggttat
     1501 tacccaattt actgatgatg tctttcagtc cattgtgacc accatggcca ccgcccaatt
     1561 taaatttggc gacgccagga ggcagatcca gttcgtcgtg ggccaccaga atttcgtccg
     1621 gattaatgcg gaaaaaactg gccatcgccg caacggcttt gccgctgaga ttcataaatg
     1681 tagtcgggac taacaggcgg acatcttcgc ctccaagagt gactcgcgaa gtataaccaa
     1741 agaatttagc ctcttcgcgc agcggagcgc gcaaacgctc tgccagtaag tcaacgaacc
     1801 aggcaccagc attatgtcgc gttgcggcgt attcagcacc ggggttcgcc aggccgacaa
     1861 tcaatttaat cgtcacgttt ttttgtcctg agtgtgtaca taactggcgc gtagtttact
     1921 ggttgcgacc ccgcttgaca aaaaactgcg tatcaaatgc agataacgta ataattgcct
     1981 gagtgggcta ttagaaagtc aaggtgttca ggcgtttatt tgtaaagttt tgttgaaata
     2041 agggttgtaa ttgtgatcac gcccgcacat aacccacagg gtgttgtcta tactttacac
     2101 ataaggaaga ggggtattcc ctgttacaac ccagaaagtt ccggaggtga catatgaaac
     2161 gcaaaaacgc ttcgttactc ggtaacgtgc tcatggggtt gggtctggtg gtaatggtgg
     2221 tcggcgtggg gtattcaatc ctcaaccagt taccacagtt taatatgccc cagtatttcg
     2281 cacatggtgc agtgctaagt attttcgtcg gtgccattct ctggctggcg ggtgcccgtg
     2341 ttggcgggca tgaacaggtg tgcgaccgtt actggtgggt tcgccactat gacaaacgtt
     2401 gccgccgtag cgataatcgc cgtcatagct aacaaataat gcagtttgct gaccagtcag
     2461 tttgcgctga ctggtcaatc tcgtacttat aaatccgcca tcgccgcgcg acgattcggg
     2521 aagaacgcga ggcgtcccga gattggttga atgcctgcgc gagccatagt gcgcagtggc
     2581 tggaattcca cgttgcacac gcgcagttca catccttcgg gcagacgctt cacaaaacgc
     2641 tggaacgcat caagaccacc agcatcaagt accggaacgg catcccactt cagaatcaca
     2701 atccgtttgc cttcaagacg tgactccagg tccgtgaaca agccttcagc agcagcaaaa
     2761 aacagcgggc caataacgcg cagtaccaga acatcgtctg gaacatctac gactaccggt
     2821 gccaggcgag tcatacgtgc gatacgacgc ataaacagca gcgatgccag cacgatcccc
     2881 acgctgatgg caataaccat atcaaacagc acggtcagcg acatgcacag cagcatgaca
     2941 atgatgtcat ctttcggtgc atgacgcagc aagtcgacca ctttatgcgc ttcactcatg
     3001 ttccacgcca ccatcaacag cagggctgcc atagcggaaa gcggcagcca ggagagcagc
     3061 ggtgccagta ccagcagggc aagaataacc agaatagagt ggatcaccgc tgagataggg
     3121 gaagttgccc cggcacggac gttagcggca gaacgcgcga tggcagctgt agcggtaata
     3181 ccaccaaaga atggagcgat aatattcccc agtccctgtc caaccagttc gctgttcgcc
     3241 ttgtgtttcg tcccggtcat accatccagt accacggcgc agagcagaga ttcgattgcg
     3301 ccgagcattg ccattgagaa tgccgcaggc agcagtgtgc gaatagaatc ccaggttagc
     3361 gtgaattctg aattaggcag atcccacggc agcaccagtt gcggcagcag ttgcggaata
     3421 ccgttaccct gagaaccatc ggccaggacg tagtggaatt gcgaaccgat ggtagcaaca
     3481 tgtccgccga gcaggttaac aatccccatc accgcgcaac cagccagcaa ggccggaagg
     3541 tgaccgggta aacgaatgcc cagacgcggc caaaaaacaa gaatacctag cgtcacaatg
     3601 ccaatggcag catcacccac attaatggtc ggcagcgcca taaataatgc gccgactttt
     3661 tgtagataat gttccgggac atgggccatt tgcagaccga gaaaatcttt aatctgcatg
     3721 gtaccgatgg tgatcccgat acccgaggtg aaacctaagg tgacggaaac cggaatatac
     3781 tcaatcaggc gaccaaagcg tgccagaccc atcagaatca aaaagatccc cgacagcaag
     3841 gccgcaacca gcagtcctgc cagtccaaac tgttgcgaca cgggatagat aattaccaca
     3901 aatgccgcag tcggaccgga aacgctaaag cgtgacccac ccgtcagagc aatgacaatc
     3961 cccgcaacag ctgcggtata taaaccgtac tggggtgcca caccactacc aatagccaac
     4021 gccatcgcca gcgggatagc aataatcccg acggttatcc cggcaatcag gtcacgggta
     4081 aaccgtgcgg cagtatattt ttctttccag caagcgtcga tcagagcgcg gaaaggcatc
     4141 acatgtgagg aaaatatttt gttcacaata atgtttcatc cgtgagcgca tcatctgtca
     4201 actaaatggc aggtgaagga ggcataggtc atacaaatgg atattacaga caaaaaaacc
     4261 cgccgcagcg ggtctttgag ccgggttcga ttagtgttcg aacatggcag agatcgattc
     4321 ttcgttgctg atacgacgaa tcgcttcggc cagcatacct gacagggtca gagtacgcac
     4381 gttcggcagt gatttgattt catcgctcag cggaatggta tcgcagacaa cgacttcatc
     4441 aattacagag ttacgcaggt tgttcgccgc gttgccagag aagatcgggt gagtcgcgta
     4501 cgcaaataca cgtttagcac cacgttcttt cagagcttca gcagctttac acagcgtacc
     4561 gccagtgtcg atcatatcat cgaccagtac gcagtcacga cctgcaacgt caccgatgat
     4621 atgcatcacc tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg ccatatcggt
     4681 atcgttcagc agcttagcga tagcgcgggc acgcacaacg ccgccgatgt ccggagaaac
     4741 cacaattggg ttatccagat tcagctgcag catgtcttcc agcaggatcg ggctaccaaa
     4801 tacgttatca accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca gatccactgt
     4861 cagcacacgg tcaacaccga cgctggagag gaagtctgca accactttcg cagtgattgg
     4921 tacacgagcg gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg ggataacagc
     4981 ggtgatacga cctgcggaag cacgacgcag ggcatcaacc ataacgacta attccatcag
     5041 gttgtcgtta gtaggggcac aagtggactg gatgatgaaa atatcaccac cgcgtacatt
     5101 ttcattaatt tgtacgctga cttcgccatc gctaaagcga cctacagcgg cgtcgccgag
     5161 tgaagtgtac aggcggttgg caatacgttg tgctagttcc ggggtggcgt taccagcaaa
     5221 aagcttcata tcaggcacga gaagaacctc aggcatgcgt ccattggtgg aaagaatctg
     5281 ccgaaaactg tgcgggccag gcatgatcct ttccaggcag tgtattaaag agcgcgatgc
     5341 aacgtctgga acaaggtgac gttgtcaccg aaactcagct tgcccggctt aaagcatggc
     5401 tctgtgcaat ggggaaagat taacgccttt cgccacaaag ccattgagcc attccggggc
     5461 ttgctctagc acctggcggg cttcagactc tgtatcaaat tcagcaaaga cacaggcccc
     5521 tgtcccagtc aggcgcgacg gggcgtattc taacagccag gaaagcaccg catcaacctc
     5581 gcgaaaacgt tttcttgcga taacctcgca atcattgctg aattcacatt ttagcaacgt
     5641 ttctattgac ctttttggcg tattgcgcgg gagttcagga tctttaaaaa tcaccggagt
     5701 cggaatactt acaccagggt gcgccaccag ataccacttc tctggcggat ccaccggcgt
     5761 tagtatttca ccaacgcctt cggcaaacgc ggcatgcccc cgaacaaaga caggaacatc
     5821 tgcgcccagc gtcagcccca tttccgccag ctcatccatg cttagcccgc attgccagag
     5881 atgatttaat gccaccagga ccgtcgcggc attggatgaa ccaccgccga gaccgccgcc
     5941 catcggcaaa cgcttgtcaa tgctgatatt cgcaccgctt cccgtcggaa gacgcccgct
     6001 gtctgccgca gttttcatca acaatcgcgc tgcgcgaacg atcaggttat cttcatgttc
     6061 cacgccttca acgggcgtta acagacgaat atccccatcg tcacgaagct caatgctgat
     6121 ggtgtcgccg taatcaagaa actgaaacag cgtttgcagc gtgtggtaac catccgcacg
     6181 ctgaccggta atgtataaaa acagattaag ttttgccgga gagggccact gtgtccgcat
     6241 tatttcacta tccagttatc catttttaac ttgatgcgtt gaccaccgtc ggtgagttcc
     6301 atattggctg gcatcgcagg atgcgttttg gtgtcataac caccataaac aaccttccag
     6361 tttttgccat tctggctgta ggtaatttcg ctcaggcggt actggtcgtc cagtttgtag
     6421 tcggttgcat cacccggtaa acctaaaatc cactggcgca agctgttgag cggaattggc
     6481 attccggtca atttgccaat catctcttcg gcgtcatcgg cggtataacg ctgaccttta
     6541 ttgtcgacta actgcacgtt acccggttga gcattcagct ccagttccgt gctgcccaat
     6601 gggttagtga gcagcagacg gtagcgatcc tggccggttt gctgccagaa aaagcgggcg
     6661 tacacttttt gttggtcaga aatataagcg aacgcgccgc gagtctgata ctgattaaga
     6721 ttgcgcacgt cttgctgatg ctgacgccat tgtggcgaat ccgggctttt gccaggacct
     6781 ttgggcgtgg taacggaaca ggcagtgagc acaagagcag ccagcggtag caggcggata
     6841 agacgaaaat cgggcagggg catagtgatg acaagtcctt gagatacgtt gcagttataa
     6901 cccttaatgc tagcgttacc gtccgctatc gtctatgttc aagttgtctt aattgccaga
     6961 atctaacggc tttcggcaat tactccaaaa gggggcgctc tcttttattg atcttacgca
     7021 tcctgtatga tgcaagcaga ctaactctat caacgttggt attatttccc gcagacatga
     7081 cccttttagc actcggtatc aaccataaaa cggcacctgt atcgctgcga gaacgtgtat
     7141 cgttttcgcc ggataagctc gatcaggcgc ttgacagcct gcttgcgcag ccgatggtgc
     7201 agggcggcgt ggtgctgtcg acgtgcaacc gcacggaact ttatcttagc gttgaagagc
     7261 aggataacct gcaagaggcg ttaatccgct ggctttgcga ttatcacaat cttaatgaag
     7321 aagatctgcg taaaagcctc tactggcatc aggataacga cgcggttagc catttaatgc
     7381 gtgttgccag cggcctggat tcactggttc ttggggagcc gcagatcctc ggtcaggtta
     7441 aaaaagcgtt tgccgattcg caaaaaggtc atatgaaggc cagcgaactg gaacgcatgt
     7501 tccagaaatc tttctctgta gcgaaacgcg ttcgcactga aacagatatc ggtgccagtg
     7561 ctgtgtctgt cgcttttgcg gcttgtacgc tggcgcggca gatctttgaa tcgctctcta
     7621 cggtcacagt gttgctggta ggcgcgggcg aaaccatcga gctggtagcg cgtcatctgc
     7681 gcgaacataa agtacagaag atgattatcg ccaaccgcac tcgcgaacgt gcccaaattc
     7741 tggcagatga agttggcgcg gaagtgattg ccctgagtga gatcgacgaa cgtctgcgcg
     7801 aagccgatat catcatcagt tccaccgcca gcccgttacc gattatcggg aaaggcatgg
     7861 tggagcgcgc attaaaaagt cgtcgtaacc aaccaatgct gttggtggat attgccgttc
     7921 cgcgcgatgt tgagccggaa gttggcaaac tggcgaatgc ttatctttat agtgtggacg
     7981 atctgcaaag catcatttcg cacaacctgg cgcagcgtaa agccgcagcg gttgaggcgg
     8041 aaactattgt cgctcaggaa accagcgaat ttatggcgtg gctgcgagca caaagcgcca
     8101 gcgaaaccat tcgcgagtat cgcagccagg cagagcacgt tcgcgatgag ttaaccgcca
     8161 aagcgttagc ggcccttgag cagggcggcg acgcgcaagc cattatgcag gatctggcat
     8221 ggaaactgac taaccgcttg atccatgcgc caacgaaatc acttcaacag gccgcccgtg
     8281 acggggataa cgaacgcctg aatattctgc gcgacagcct cgggctggag tagcagtaca
     8341 tcattttctt tttttacagg gtgcatttac gcctatgaag ccttctatcg ttgccaaact
     8401 ggaagccctg catgaacgcc atgaagaagt tcaggcgttg ctgggtgacg cgcaaactat
     8461 cgccgaccag gaacgttttc gcgcattatc acgcgaatat gcgcagttaa gtgatgtttc
     8521 gcgctgtttt accgactggc aacaggttca ggaagatatc gaaaccgcac agatgatgct
     8581 cgatgatcct gaaatgcgtg agatggcgca ggatgaactg cgcaaagcta aagaaaaaag
     8641 cgagcaactg gaacagcaat tacaggttct gttactgcca aaagatcctg atgacgaacg
     8701 taacgccttc ctcgaagtcc gagccggaac cggcggcgac gaagcggcgc tgttcgcagg
     8761 cgatctgttc cgtatgtaca gccgttacgc cgaagcccgc cgctggcggg tagaaatcat
     8821 gagcgccagc gagggtgaac atggtggtta taaagagatc atcgccaaaa ttagcggtga
     8881 tggtgtgtat ggtcgtctga aatttgaatc tggcggtcat cgcgtgcagc gtgttcctgc
     8941 tacggaatcg cagggtcgta ttcatacttc tgcttgtacc gttgcggtaa tgccagaact
     9001 gcctgacgca gaactgccgg acatcaaccc agcagattta cgcattgata ctttccgctc
     9061 gtcaggggcg ggtggtcagc acgttaacac caccgattcg gcaattcgta ttactcactt
     9121 gccgaccggg attgttgttg aatgtcagga tgaacgttca caacataaaa acaaagcgaa
     9181 ggcgctttca gtgctcggtg ctcgcatcca cgctgctgaa atggcaaaac gccaacaggc
     9241 cgaagcgtct acccgtcgta acctgctggg gagtggcgat cgcagcgacc gtaaccgtac
     9301 ttacaatttc ccgcaggggc gcgttaccga tcaccgcatc aacctgacgc tctaccgcct
     9361 ggatgaagtg atggaaggta agctggatat gctgattgaa ccgattatcc aggaacatca
     9421 ggccgaccaa ctggcggcgt tgtccgagca ggaataatgg aatatcaaca ctggttacgt
     9481 gaagcaataa gccaacttca ggcgagcgaa agcccgcggc gtgatgctga aatcctgctg
     9541 gagcatgtta ccggcagagg gcgtactttt attctcgcct ttggtgaaac gcagctgact
     9601 gacgaacaat gtcagcaact tgatgcgcta ctgacacgtc gtcgcgatgg tgaacccatt
     9661 gctcatttaa ccggggtgcg agaattctgg tcgttgccgt tatttgtttc gccagcgacc
     9721 ttaattccgc gcccggatac ggagtgtctg gtggagcagg cactggcgcg gttgcctgaa
     9781 caaccttgcc gtattcttga tctcgggacg ggtaccgggg cgattgcgct tgcgctggct
     9841 agcgagcgcc cggactgcga aattatcgct gtagatcgta tgcctgatgc tgtctccctg
     9901 gcacaacgta atgcccagca tctggcgatc aaaaatatcc acattctgca aagcgactgg
     9961 tttagcgcgc tagccgggca gcagtttgcg atgattgtca gcaatccgcc gtatattgac
    10021 gagcaggacc cacatcttca acaaggcgat gtccgctttg agccgctcac tgcgctggtt
    10081 gcggcagaca gtggaatggc agacatcgtg catatcatcg aacagtcgcg taacgcgctg
    10141 gtatccggcg gctttctgct tctggaacat ggctggcagc agggcgaagc ggtgcgacaa
    10201 gcatttatcc tcgcggggta tcatgacgtc gaaacctgcc gtgactatgg tgataacgag
    10261 cgcgtaacgc tcggccgcta ttatcaatga caagtttttc tacactgctt agtgttcatc
    10321 ttattagtat cgcgctttct gttgggctat taaccttacg tttctggcta cgttatcaga
    10381 agcatccaca ggcatttgct cgctggacgc gcattgtgcc gccggttgtc gatacggtgt
    10441 tattgttaag cggcattgcg ttgatggcta aagcgcacat cctgccattt tccgggcagg
    10501 cacagtggct gactgaaaag ctgtttggag ttatcattta tatcgttttg ggttttattg
    10561 cactcgatta tcgtcgtatg cacagtcagc aggcgcgcat tattgccttc ccgctggcgt
    10621 tggtggtgct gtacatcatc attaaactcg ccaccacaaa agtaccgtta ctggggtaag
    10681 tcatgagatc gttagctgat ttcgaattta ataaagcgcc attgtgcgaa ggcatgatcc
    10741 tggcttgcga agcaatccgc cgcgattttc cctcgcaaga tgtttacgac gaactggagc
    10801 gtctcgttag tctggcgaag gaagaaatca gccagcttct gcctttagaa gagcagctgg
    10861 aaaaactgat cgcgctgttt tacggcgact ggggatttaa agcctcacgc ggcgtttatc
    10921 gtctttccga tgcattatgg ctggaccagg tgttaaagaa tcgacagggc agtgcggtat
    10981 cattaggtgc ggttttatta tgggtcgcga atcgtctcga tttgccgctg ctgccggtga
    11041 ttttccctac gcagctgata ttgcgcattg aatgtccgga tggcgaaatt tggctgatta
    11101 atccttttaa cggtgaatcg ttaagcgaac atatgctgga cgtatggtta aagggaaata
    11161 tcagcccgtc ggcggaactg ttttatgaag accttgatga agctgataac attgaggtaa
    11221 tccgcaaatt gctggataca ctcaaagcct cgttgatgga agaaaatcag atggagctgg
    11281 cgttacgcac cagcgaagct ttattacaat tcaaccctga agatccctat gaaattcgcg
    11341 atcgcgggtt gatttatgcg caactggatt gcgaacacgt tgcgttgaac gatttaagtt
    11401 atttcgttga acagtgtccg gaagacccga tcagcgaaat gatccgtgcg caaataaata
    11461 acatcgcgca taaacatatt gtgctgcatt aattaatcga cattttactc aagattaagg
    11521 cgatcctatg aaacaaaaag tggttagcat tggcgacatc aacgtagcaa atgacctgcc
    11581 gttcgtactg tttggcggta tgaacgtgct ggaatctcgc gatctggcga tgcgcatttg
    11641 cgagcactac gtaactgtga cccagaaact gggtatccct tacgtgttca aagcctcttt
    11701 tgacaaagcc aaccgctcct ccatccactc ttatcgtgga ccgggcctgg aagaagggat
    11761 gaaaatcttc caagagttga agcagacttt tggcgtgaaa attatcaccg acgttcacga
    11821 accaagtcag gcacagcccg ttgctgatgt cgtggatgtg attcagttgc cggcgtttct
    11881 tgctcgccag actgacctgg ttgaagccat ggcgaaaacc ggtgcggtaa ttaacgtcaa
    11941 gaaaccacag tttgtcagcc cgggacagat gggtaatatc gttgataaat tcaaagaagg
    12001 cggcaacgaa aaagtgattc tttgcgatcg tggtgctaac ttcggctatg acaacctggt
    12061 tgtcgatatg ctgggcttca gcattatgaa gaaagtgtct ggtaactcgc cggtgatttt
    12121 cgacgtgacc cacgcactgc aatgccgcga tccgtttggc gcagcttccg gtggtcgtcg
    12181 tgctcaggtg gctgagctgg cacgagccgg tatggcggta ggtctggcgg ggctgtttat
    12241 tgaagcgcat ccggatccgg aacatgcgaa atgtgatggt ccatccgcgc tgccgctggc
    12301 taaactggaa ccgttcctca agcagatgaa agcgattgat gatctggtga aaggtttcga
    12361 agaactggat accagcaagt aatctttttt gcttgaaaaa taaagtctta gcgttctgcg
    12421 ttaagacttt tttcatgggt gccagataca aaaaaggccg caggctgtta cccctgcggc
    12481 cggtttcggg cgcatattgc catcacggca gcctgacgcc cgttttcacc ttacttccgg
    12541 ttacgccacc agccgacaat cgctgcggta ataattcccg ccaggatcgg tgctgccagg
    12601 tcgtgccaga aaatcatggc aaactgcgcg agcgtcatat agccgccttg ttgtaatgac
    12661 aacattttgc ggctattctt gaattgttct ggttcaagat tagcccccgt tctattgtca
    12721 ggttaaacct ctcaacgtgc gggggttttc tctttccagc aaccaatgcc accagggata
    12781 aagcccccgc aacattgcgc ctcaccggat aacgccggct tgatgtggat actacgtctc
    12841 aattcatctt cacttcatcc ctgaaatgtt tgcaatgaag agtgcattcc ggtttttcaa
    12901 cagctgttac agtcgtttca tgagtgctct ggatgatgct tccagctcgg gttgccaata
    12961 tttacttgtg gaagagataa agacaaaaat ggccgcaggc tgttacccct gcggccggtt
    13021 tcgggcgcat attgccatca cggcagcctg acgcccgttt tcaccttact tccggttatg
    13081 ccaccagccg acaatcgctg cggtaataat ccccgccagg atcggtgctg ccaggtcgtg
    13141 ccagaaaatc atggcaaact tcg
//
LOCUS       DAJTSL010000111        13097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTSL010000111 DAJTSL010000000
VERSION     DAJTSL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.111"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            7..1635
                     /locus_tag="OO433_002871"
     CDS             7..1635
                     /locus_tag="OO433_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR6051278.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKQSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLIQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRI
                     LAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVKLAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1874..1933
                     /locus_tag="OO433_002872"
     CDS             1874..1933
                     /locus_tag="OO433_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6051279.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2250..2309
                     /gene="ibsE"
                     /locus_tag="OO433_002873"
     CDS             2250..2309
                     /gene="ibsE"
                     /locus_tag="OO433_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR6051280.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2429..3862)
                     /gene="hldE"
                     /locus_tag="OO433_002874"
     CDS             complement(2429..3862)
                     /gene="hldE"
                     /locus_tag="OO433_002874"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6051281.1"
                     /translation="MKVTLSEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3910..6750)
                     /gene="glnE"
                     /locus_tag="OO433_002875"
     CDS             complement(3910..6750)
                     /gene="glnE"
                     /locus_tag="OO433_002875"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6051282.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFN
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMMLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6773..8074)
                     /gene="ygiF"
                     /locus_tag="OO433_002876"
     CDS             complement(6773..8074)
                     /gene="ygiF"
                     /locus_tag="OO433_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6051283.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8316..8936
                     /locus_tag="OO433_002877"
     CDS             8316..8936
                     /locus_tag="OO433_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6051284.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKERWMN"
     gene            9000..10238
                     /gene="cca"
                     /locus_tag="OO433_002878"
     CDS             9000..10238
                     /gene="cca"
                     /locus_tag="OO433_002878"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6051285.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10395..11216)
                     /gene="bacA"
                     /locus_tag="OO433_002879"
     CDS             complement(10395..11216)
                     /gene="bacA"
                     /locus_tag="OO433_002879"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6051286.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11307..11675)
                     /gene="folB"
                     /locus_tag="OO433_002880"
     CDS             complement(11307..11675)
                     /gene="folB"
                     /locus_tag="OO433_002880"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6051287.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11780..12397
                     /gene="plsY"
                     /locus_tag="OO433_002881"
     CDS             11780..12397
                     /gene="plsY"
                     /locus_tag="OO433_002881"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6051288.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12410..>13097)
                     /gene="ttdR"
                     /locus_tag="OO433_002882"
                     /gene_synonym="dan"
     CDS             complement(12410..>13097)
                     /gene="ttdR"
                     /locus_tag="OO433_002882"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCR6051289.1"
                     /translation="LVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQ
                     VHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYPQPQS
                     LQELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKGIM
                     LRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLVAWCQQRL
                     GKPDEGYQVM"
BASE COUNT         3165 a         3682 c         3280 g         2970 t
ORIGIN      
        1 tcctggatgt ttaccgcgat tattgccgta tgcattctgt ttattattgg aattattttc
       61 gctaggctct atcgtcgcgc ttcggcagag caagcttttg ttcgtactgg tttaggtggg
      121 caaaaagtgg tgatgagcgg tggcgcaatc gtgatgccga tctttcatga aataatcccc
      181 atcaatatga atactctgaa gctggaagtc agccgctcaa ccattgatag cctgattacg
      241 aaagatcgta tgcgtgtcga tgtagtagtc gctttctttg tacgggtaaa acagtctgta
      301 gaaggcatcg ccactgcggc gcagacgttg gggcaacgca cgctatcacc agaagactta
      361 cgtatgttgg ttgaagataa atttgtcgat gccctccgtg caacagctgc acaaatgacc
      421 atgcatgagt tacaggatac ccgcgagaac ttcgtgcagg gagtacaaaa tactgtcgca
      481 gaagacctgt cgaaaaacgg tcttgagctg gaaagcgttt cacttaccaa ctttaaccag
      541 acctcgaaag aacatttcaa tccgaacaat gcctttgacg ccgaaggttt aaccaaactg
      601 attcaggaga cggagcgccg tcgtcgcgaa cgtaacgaag ttgaacagga tgtagaagtt
      661 gcggtgcgtg agaaaaaccg tgatgcactt tcgcgcaagt tggagattga acaacaagaa
      721 gcgtttatga cgcttgagca ggagcagcag gttaaaaccc gtactgccga acagaatgca
      781 cgtattgcgg cttttgaagc tgaacgtcgt cgtgaagcag agcagacgcg aattctggct
      841 gaacgacaaa ttcaggaaac ggagatcgag cgcgagcagg ccgtccgctc aagaaaggtt
      901 gaggctgaac gtgaagttcg tattaaagag atcgaacagc agcaggtcac cgaaatcgcc
      961 aaccagacga aatcgatcgc tattgccgcc aaatcggaac aacagtccca ggcagaagcg
     1021 cgtgctaacc ttgcactcgc agaagcagta agtgcgcaac aaaacgtaga aaccactcgc
     1081 cagaccgcag aagccgatcg tgctaaacaa gttgccctaa tcgctgccgc gcaggatgca
     1141 gaaaccaaag cggttgaact gaccgtgcgg gcgaaagcag aaaaagaagc cgcagagatg
     1201 caggcggcgg ctatcgttaa gttagccgaa gctacacgta aaaagggtct ggcggaagca
     1261 gaagcacaac gtgcgctgaa cgatgctatc aacgtacttt ctgatgaaca aaccagcctt
     1321 aaattcaaac tggccttgtt gcaggcgcta cctgcggtaa tagaaaaatc cgttgagccg
     1381 atgaaatcta ttgacggtat caagattatt caggtcgatg gtctgaatcg tggcagcgct
     1441 gcgggtgatg caaacacggg taatgtgggg ggcggaaacc tggcggaaca agcattatca
     1501 gccgctctct cttaccgcac acaggcaccg ctgattgact ccttgctcaa tgaaattggc
     1561 gtttcaggcg gctcactggc ggcattgact tcacccttaa cctcaacaac tcccgtcgcc
     1621 gaaaacgtag aataaatttt cagctatgaa cgtccctggc acaaaaagcc ggggattttt
     1681 tatatctgcg ttccgctaaa aactgcaaat gatcaggtcg ttgcaacgtt ttgcgtgacc
     1741 gctcggggaa ggcaaaactg cctctgggaa agcgttgctc gaggtccggc gctcatcaac
     1801 aatcggggag tcagcaaagg gctgaaacgg gaaagcccct cccgaggaag gggccttaca
     1861 gaaggaaagg gttatgatga agctcgtcat catactgatt gtgttgttac tcgtaagttt
     1921 cgcagcttat taacagccaa tcagaggggg gagaaatcct ccctcaccct tgttccttta
     1981 ctttaggttg aaaaaaaatc agcgtcaaga aggtttcatt aacacatatc cctagcccaa
     2041 aaagccgggg attttttata tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc
     2101 agcgttttgc gtgaccgctc ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg
     2161 tccggcgctc atcaacaatc ggggagtcag caaggggctg aaacgggaaa gcccctcccg
     2221 aggaaggggc cttatagaag gaaagggtta tgatgaagtt cgtcatcata ctggttgtgt
     2281 tgttactgtt aagtttcccg acttactaac aactgatcag aggggggaga aatcctccct
     2341 tacccttgtt cctttacttt aggttgaaaa aacaacagcg tcaataggcc tgcctgctac
     2401 gaagcgagat ctgtgaaccg ctttccggtt agcctttttt atcctgttgg atcttcttga
     2461 tgatgttagt ggtcgagcaa ccgtcttcga agttgagcac cagcacttcg ccgccgttgg
     2521 cccagacttc tttactcccg gcgatctctt ctggtttata gtcgccgccc ttcaccagta
     2581 aatctggcag gatcccggcg atcaagcgct gcggcgtgtc ttcttcaaac gacaccaccc
     2641 agtcgaccgc ttccagtgcg cccagcacaa tcatacgctg ttcgagcggg ttaacggggc
     2701 gggaatcccc tttcagccgt ttggtggagg catcgctgtt cacagcgaca atcagacggt
     2761 cacccagttt gcgagcattt gccagatagg agacgtgccc ggcatgcagg atgtcaaaga
     2821 caccgttggt cattaccacc ttttcgccgc gtttacgcgc tgccgctacg gccagcttca
     2881 gttcctcttc ggtcatcacg ccaaagccgg tatctgcgcg cccacgcact gcattttcca
     2941 gctcgatcgg cgaaaccgtg gaggttccca gtttgccgac caccacgcca gccgccgcat
     3001 tggcaaagaa gcaggcttct tccagcgaat tacccgctgc cagcgttgcc gccaggacgc
     3061 caatcaccgt gtcgcccgca ccggtaacgt catacacttc ctgcgcctgg gttggcatat
     3121 gcagcggcgc tttacccggt tgcagcagcg acataccctg ttcggaacgg gtcactaaca
     3181 gagccgagag ttcgtaatcg gcaatcagtt tcatgccgcg ctcaacaatc tcttcttcgg
     3241 tcttacattt accgacaaca gcttcaaatt ccgagagatt cggcgttaac agcgtagcgc
     3301 cgcggtagcg ctcaaaatcg gtaccttttg gatcaatcag caccggaacg cccgctttac
     3361 gcgccagttg gatcatctgc tgtacgcttg ccagcgcacc tttggcgtag tcagaaagca
     3421 ccagcgcgcc aatcgaactc agcgcctgat taatccgctc gtgcagcggc tgcggatcaa
     3481 cgccttcgaa accttcttcg aaatccagac ggatcagctg ttggttgcgg gaaagtaccc
     3541 gtaatttggt gatggtcgga tgcgtcggta cagaaacgaa gtcgcatttg acgttgacgt
     3601 cggccagaga tttactcagc gcgcgcgctg catcgtcaat gcccgtcaac ccgaccaggc
     3661 gtgcattagc accgagagaa gcgatattca tcgccacgtt agccgcgccg cccggacgtt
     3721 cttcgatggt attcacttta accacgggca ccggcgcttc cggcgagata cggctggtgg
     3781 gaccgtacca gtaacgatcc agcattacat caccaaccac catcactcct gcacgttcaa
     3841 actctgacag cgttactttc attcctgtct cctgagagat tcaaaatttg cgcgcgataa
     3901 taccatactt cattcttcca ccagccactt ctgccagctt gcccgcacca gatcacgctc
     3961 tgcggtgaag caatcctccg acacatggcc cggcaattcc tgtaatgcca gatgatgaag
     4021 ttcatcgcgc agcgtcgtgt aagcacgggt cagcgccatc gcttcctgct cttccataat
     4081 gtcgttttgc gccagcagct ccagaatacg cacgttgtct gaccagcgcg ttaacttcgg
     4141 tttttcatga gcgtagcgca acaccagata ttgggtaata aattcgatat cggtaattcc
     4201 gccttcatcg gctttgatat caaagcgatt acgatgttta ttgccgagat gagcgcgcat
     4261 tttctcgcgc atttcccgca cttccgtttg cagagtttta ccttcacgcg gcagcatcat
     4321 aatctcgcga cgcactgcgt caaagtgcgc ggtgagctgc ggatcgccgt acactacacg
     4381 cgcacgcacc agcgcctgat gctcccacgt ccaggcctcg tttttctgat aatcagcaaa
     4441 tgcttctgcg gatgtcacca gcattcccgc cgccccggac ggacgcagtc gtgcatccac
     4501 ttcatacaaa atgccggaag aggttcgcgt attgaacaga tgcataatgc gttgcgccag
     4561 acgcagataa aactgtcgcc cgtcgatttc ccgctcgccg tcagtcatcg catccattgg
     4621 gcaatcatgg aggaagatca ggtcaagatc ggaactgtag cctaactccc agccgcccag
     4681 cttgccgtag ccgaccaccg caaaaccacg gccttcgcga tcgttgaggt gatttggctt
     4741 accgtagcgg gccaccattt gaacccacgc ctgctgaacg acggcatcta tcatagcctc
     4801 tgccagccag gttaagtgat cgctcacttt catcaccggt agcgtaccag cgatatccgc
     4861 tgcggcgatg cgtaacagct gcgcctgttt gaactgacgc agcgcctcaa gctgttgctc
     4921 ttcgtcatct tccggcacgc gcagcaaata ctggcgcaac tcatcgcggt aggcatcggt
     4981 cgccgtcggc tggtaaagag tgtttggatc gagcaattca tccagcaata atggataacg
     5041 cgccagctgg ctggcaatca tcggcgatgc ggcacacaga gaaatcaaat gtttaagcgc
     5101 cgcagggaat tcactgagca attctaaata ggtggtgcgg gtaacaatcc ccaccagcaa
     5161 ggcggtaatg cgcgacagcg taacggcagc gtcttcacgc gcacagacat cacttagcag
     5221 atgtggcatc agatggtcga gcacctgacg tcctcgcggc ccgatagtac gcttatccag
     5281 ctctttgcgg aaatcagcaa tcagcgttag gacctgtttg cgatcatcct cgctaagatg
     5341 cgccagcact ggcgtagtgt catcttcctg caacgcatcc tgccacagct cacgccactg
     5401 ttccgacagc gactcttcct gagtttcact ttcgtcgtcg ccaatcaatt cattaaacac
     5461 ccggcgcaca ttggtcatat gtgcggtcag cgccccggtc agttgcggcc agtcagcaaa
     5521 gtccatcgcc cacgccagcc gcgcacggtt aagctcatca gaaggaagcg tttgggtttg
     5581 ttcgtcgtta atgctttgca gcaggttttc cagacgccgc aggaacagat atgccactcg
     5641 caattgttca gcatcgtttt cagaaagcag atgcagcgcg gcaatgacgc tgagcgttgg
     5701 tagtaaagag cgcgactgca gcgacggttc gcgcccaccg cgaatgagct ggaacacctg
     5761 aacgataaat tcaatttcgc gaatgccgcc tgcgccgagt ttgatattgt cggtcaaacc
     5821 acgtcgacgc acttcacggg caatcatccc tttcatgttg cgcagcgact gaatcacgct
     5881 gaaatcgatg taacgacgga aaacaaacgg gcgcagcatc gcacgcaact cgttagcata
     5941 gacgccttcg ctatcgccca taatccgcgc cttgaccatc gcgtaacgct cccagtcgcg
     6001 cccctgctcc tggtaataat cctccaacgc ggcaaagctc agcaccagcg ggccactttc
     6061 gccaaacgga cgcagccgca tatccacgcg atagacgaag ccatcctgcg ttggttgatc
     6121 caacacttta atcagccgct gccccatgcg ggtaaaaaac tgcgcgttat ccagttcccg
     6181 gcgtccgccc tgcgtacaac catgttccgg ccaggcaaaa atcagatcga tatctgagga
     6241 gaaattcagt tccccaccgc ccagcttacc catgcctaaa atcagcagcg gttgcgcttc
     6301 gccctgcgca ttgcacggcg ttccccactc gcggcagcaa gcgtcataca gccagtcacg
     6361 cgcggcgaca atcagcgtct ccgccagatg gctgagctgc tgcaatatac tctcttcagt
     6421 aaccaaagcc agcgtttgcg cccaggcgat gcgcaccata atgcgccgcc ggaatagccg
     6481 cagctcgcgc attaacccgg cttcgtcact cacattgctg agcgcctcct gcaaccatgc
     6541 cgcgtaatgc tgccattcat cagcctgcgg cgattggctt tccagttccg tcagccactc
     6601 tggatgcgca atcacgctgt cctgcacaaa atcactgaaa gtaagtactg acttcgcctg
     6661 tgcgctaagt gatttctcgg ctaaaggctc tggcagccgc tcaacaacgg tctgccagta
     6721 ctgctgtaac ggtgaagaga gcggcttcat aagcgatttt atccttgcct gattaacgtt
     6781 ttccgctgtg caaccagaac ggttcctgat tgtttgcctc attacggaaa tgttcaattt
     6841 caatgcgttg cccggtcgca atagcgtgac gcagcccctg ccagttctcc agccaggctt
     6901 gcgcgacgac aggatcatag taacccgcca gcaacagtat tgagtcaata tcacgcgtca
     6961 gacgtggcaa ctggtcacgg tagcgatcgc ccaacggctg gcagaaaacg cttttcagtt
     7021 cagcggcatg gcgggaaaga tggatatcgg caaagcgttt gaaggaatcg ctcattttac
     7081 tctgcgcttt ggcatctaaa aacggctgcc aggctttgct taccagccat tcggtcaacg
     7141 ccagcttcgc cattgccgtt ttggtagagt agaccgccgt cacggcagaa accgcagaag
     7201 cgatggtcgc ttcgcattga gtcagcaaat cgcgtaagtg agtgctcgct ttgcgcggca
     7261 caataccacc gaacagcatc agtgtatgac ggaccagacc aatggctgcc agcacctgtt
     7321 ctttcgccgc atcgttgccg cgtacccaca gttcttcatg atactgccat tgcgctaacg
     7381 ccagctcgag cgccgcttcc agcccctgtt ccacatcggc ttttgccgca acatgcaaaa
     7441 tggtggtcgg tttgatttca cgcgccggat tgccctgcgc cagatgatag ccacgcgccg
     7501 ctttgctcag gctgccctgg cgtaatccgg tttgcgatac cagttggttc gccagtttca
     7561 gcaccgcgcg cgtgtcgccg ctaagcagtt ccagttccag ctcacagata ggttcagcaa
     7621 attcccccgc tttcacctcc ccctgatcga gagcgatttc aattcggctg tcatcgaccg
     7681 ccaccagcca tttttcgcga taaaaatcgg tgctgaacag cggttgtacg cgggaagcga
     7741 gatcggcggg caattcgccg ttcggccaga cttccgtcgg taactgcgcc aggtcgagcg
     7801 tcggttcgct caacgccacg ttatattccg ggcgctggtg taagccgcct gtcactcttc
     7861 ctgcaacttt catggtcatc tcatagcgac cgttttcgcc acgaatacgt aagcccatat
     7921 cgtgcccacg cagccagtta tccggcgttt cgtagtaaat attcagcaac tgcacggggt
     7981 catggtgctc gccgcccagc gtattgagat ggtcacgcaa cgcctcaacg gcactgtgat
     8041 taacaataaa ctttaattcg atttcctgag ccatggcctt tacttatcgg ttatgtcaca
     8101 taagtgacga tgaacggcga acttaatgcg atcttttttg tcagtagata gtattttgcg
     8161 ccaaattgcc atgcaacggg caatttgacg ggcgtaaaag tttgaagcag tggcaagacc
     8221 tgacacagag gattccgatt gatgtcgaat gctttgcgta gcgacactga tgccactact
     8281 atcgttccac tttccatgac aataacgaca gcctgatgcc aaaattacgc ctgatcggat
     8341 taactttact cgcacttagc gccactgccg tctcacacgc tgaagaaacg cgctatgttt
     8401 ctgatgaact gaatacctgg gtccgtagcg gtccgggaga tcattatcgc ctcgtgggca
     8461 cggttaacgc cggcgaggaa gtgaccttat tacaaactga cgccaacacc aattatgccc
     8521 aggtgaaaga cagctctggc cgtaccgcct ggatcccgtt gaaacaactt agcactgagc
     8581 caagcctgcg ctcccgtgtg ccagatctgg aaaatcaggt caaaaccctg accgataaac
     8641 tcaccaatat cgataacacc tggaatcagc gcaccgcaga aatgcagcaa aaagtggcgc
     8701 agagcgacag cgtgatcaac ggattaaaag aagaaaatca gaaattgaaa aacgagctga
     8761 ttgtcgcgca gaaaaaggtc gatgccgcca gcgtacagct ggatgacaaa cagcgcacca
     8821 tcatcatgca atggtttatg tatggcggtg gcgtgctggg gcttggcttg ctgctcggtc
     8881 tggtactgcc acacctgatc ccaacgcgca aacgcaaaga gcgctggatg aactaaatcg
     8941 ccttcttcgt tgcactgtct tattattagg aattgtgtat ttgacgaagg gatgatggcg
     9001 tgaagattta tctggtcggt ggtgctgttc gggatgcatt gttagggcta ccggtcaaag
     9061 acagagattg ggtggtggtc ggcagtacgc cacaggagat gctcgacgcg ggctaccagc
     9121 aggtaggccg cgattttcct gtgtttctgc atccgcaaac gcatgaagag tatgcgctgg
     9181 cacgtaccga acggaaatcc ggttccggtt acaccggttt tacttgctat gccgcaccgg
     9241 atgtcacgct ggaagatgat cttaagcgtc gcgatctgac cattaatgcg ctggcccagg
     9301 acgataacgg cgagattatc gacccgtaca acggtctggg cgatctgcaa aatcgtctgt
     9361 tgcgccatgt ttcccccgct tttggcgaag atccgttacg cgttctgcgc gtggcgcgtt
     9421 ttgctgcgcg ttatgcccac ctcggttttc gtattgccga tgaaactctg acgctgatgc
     9481 gcgagatgac ccatgcgggt gaactggaac acctgacgcc tgaacgggta tggaaagaga
     9541 cggaaagcgc cctcaccacc cgcaatcccc aggtgttctt ccaggtactg cgcgattgcg
     9601 gtgcacttcg cgttttattc ccggaaattg acgccctgtt tggcgttccg gcccctgcca
     9661 ggtggcatcc ggaaatcgac accggtattc ataccttaat gacgctctca atggcggcga
     9721 tgctgagtcc gcaggttgat gtccgtttcg caactttatg ccacgatctc ggtaaaggac
     9781 tgacgccgcc agaactttgg ccgcgtcatc atgggcatgg cccggcgggt gttaagttag
     9841 tggaacaact atgccagcgt ctgcgcgtgc caaatgaaat tcgcgattta gccagactgg
     9901 tggctgagtt tcacgatctc atccacacct tcccaatgct gaacccaaaa accatcgtca
     9961 aattatttga ttccatcgat gcctggcgta aaccgcagcg tgtcgagcaa ctggcgctga
    10021 ccagcgaggc tgacgtacgc ggcagaaccg gttttgaatc agcggactac ccgcaaggcc
    10081 gttggttgcg cgaagcctgg gaagtggcgc agtcagtgcc gacaaaagcg gtcgttgaag
    10141 cgggatttaa aggtgtggag attcgcgagg agctgacccg acggcggatt gcggcggtag
    10201 ccagttggaa ggaacaacgt tgcccaaagc ctgactgagt ataaaactaa acgttaacac
    10261 gaaccatccc ctcgcccctt cggggagagg gtgaggggaa cagaccagca ccggtgcgaa
    10321 caatcacccc caccccggcc ctctccctac agagcgaggg agaaaaccag gcctgctccc
    10381 gaaccttttc tcaatcaaaa gaacacgacg tacaccgcag ccgccacaat aaagcgataa
    10441 atggcgaacg ggataaacga aatgcgctta atcaattgca ggaaggtttt aatcgctatc
    10501 agcgccacca caaaagcggt gataaaccca acggcaaaca tcgggatatc gccggttgtc
    10561 aggaagcccc agcttttgta gagatcgagc gccgttgcgc ccatcatcat cggtaccgcc
    10621 agcaggaacg agaactcgga agcagcgtaa cggctcacgc ccatcagcat cccacctgaa
    10681 atggtggccc cggaacggga gaaacccggc cacagcgcca gacactggaa acagccaatc
    10741 ataaacgcct gacgataggt catatcatca agacccggcg cacgcggctc tttcggcttc
    10801 aggcattcgg cggcaatcag caacaaaccg ccaacgacca gtgcatacat cacatttatc
    10861 gggttaaaca atgacttaat cgtgtcgtgg aacaacagcc ccagtaccac cgccggaatc
    10921 atccccagca aaatgtggat cagcgttaaa cgacctttgc tttctccttc gtgctgcaac
    10981 gggcggccaa agtggatgcc aatcaggcca aacagacgcc gccagaacat cactactacc
    11041 gccagaattg atcctaactg gatcacaact tcaaaggttt tcgccgtgtc gccctcgaac
    11101 cccaacaagt gaccgacaat aatcatatgg cccgtgctgg ataccggcag aaattctgtc
    11161 aatccttcga ccacacccaa tattgccgct atcagcagcg agtgcatatc gctcatcaat
    11221 aaacccctaa attattaaaa tgtaccgctt gtccggacta ctgcagtaag accaggttat
    11281 aaccgtttgg tttaacagct gtaaaattaa ttattttctt tcagattatt gccacgctca
    11341 atgattacac caacattcgc cgcccgcgcc actgcgcctg gcttgctgag tttgatgcgc
    11401 acccacggcg agttgaagcg tgctaacagc agctccgcca cctcttcagc cacgcgttcc
    11461 accagcgcaa aacgcgcccc ctcgacgtgg ctgaccaccg tttctgcaat gtcagcgtaa
    11521 ctgaggcaat ccgccacatc atcacttttc gccgctttac ggttatccca tgccatttcg
    11581 atatcgaaca ctaacttctg ttcgatggtc tgttcccagt cgtaaacacc aatagtggtg
    11641 attaccgaaa gttgctctat aaatacaata tccatcacgt cctgcctgct ttttggctaa
    11701 ccggatacca cttccggcga aatgtgcgta ttatccacag attcatcgtt gaacacgaat
    11761 tttcaaaacg gaacagctta tgagtgcaat cgcgcctgga atgatcctca tcgcgtacct
    11821 ctgcggctcc atttccagtg ccattctggt ttgccgcttg tgtgggctgc ccgatccgcg
    11881 aaccagcggc tccggcaatc caggcgcaac caatgtgtta cgtatcggtg gcaagggagc
    11941 agccgtagca gtactgattt tcgacgttct gaaaggaatg ttgcccgtct ggggcgcgta
    12001 tgaattaggt gtcagcccct tctggctagg cttaattgcc atcgccgcct gtcttggaca
    12061 catctggccc gttttcttcg gatttaaagg aggaaaaggc gttgctaccg cttttggtgc
    12121 catcgcaccc attggctggg atctcaccgg agtaatggcg ggaacctggt tactgaccgt
    12181 gctattgagc ggatactcgt cgctgggagc gattgtcagt gcactgattg ctccgtttta
    12241 tgtctggtgg tttaagccac aattcacctt cccagtttcg atgctctctt gcctgatcct
    12301 gctgcgtcat catgacaaca tccagcgtct gtggcgtcgt caggagacaa aaatctggac
    12361 gaaattcaaa agaaagcgcg aaaaggatcc cgagtgattt ctggtggatc tacatgacct
    12421 gatagccttc atcgggcttg cccagccgtt gctggcacca tgccaccaga aattccacgc
    12481 agacacgtaa tttcatgctg cgatagagcg gctcccggta aacagcccag atattggcgc
    12541 tttgtgcata ctccggcaat acttgcacca gtttgccact ctccagaaac ggcaacacat
    12601 cccactcgga acgcagcata atccctttgc cctccagcgc ccattgcaac actatctcgc
    12661 cgctattgga ggaaagatgc ccgctaacct tcaccgattt tttctcctga ccgttcccca
    12721 actcccatat tccgtgggtc atatcgcgct ctttggtcac cagacagtca tgacgactta
    12781 attcctgtaa tgattgtggc tgcggatatt tttgcagata ctcaggtgct gcgcataata
    12841 ttcttttatt tttcgttaac agatgcgcaa tataataatc aggaatttcg tcattaatac
    12901 gaatatccag atcaatatta tcctgcacta aatcaatttg ccgatcgaac aattcaaaat
    12961 gcacctgtaa ctcaggataa ttgcgcataa gttcggtaat ggctggcgca atatggctgc
    13021 gcccaaaacc aaaactgcaa ccaatacgaa tcatcccctc cgggcgcgtt ttgatttgcg
    13081 tgacgtcatc gaccagt
//
LOCUS       DAJTSL010000112        12952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTSL010000112 DAJTSL010000000
VERSION     DAJTSL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.112"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO433_002883"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO433_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6051290.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGNTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="OO433_002884"
     CDS             1946..2230
                     /locus_tag="OO433_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6051291.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="OO433_002885"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="OO433_002885"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6051292.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFACEVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGAWMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="OO433_002886"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="OO433_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6051293.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTVQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTTLEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTKGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="OO433_002887"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="OO433_002887"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6051294.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7227..7487
                     /locus_tag="OO433_002888"
     CDS             7227..7487
                     /locus_tag="OO433_002888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6051295.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDT"
     gene            7494..7790
                     /locus_tag="OO433_002889"
     CDS             7494..7790
                     /locus_tag="OO433_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6051296.1"
                     /translation="MTTTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSLGTSY
                     KSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7872..10514
                     /gene="yfcU"
                     /locus_tag="OO433_002890"
     CDS             7872..10514
                     /gene="yfcU"
                     /locus_tag="OO433_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCR6051297.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQIRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQINYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            10534..11286
                     /locus_tag="OO433_002891"
     CDS             10534..11286
                     /locus_tag="OO433_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6051298.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNSCQVDEEQSRKG"
     gene            11302..11811
                     /locus_tag="OO433_002892"
     CDS             11302..11811
                     /locus_tag="OO433_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6051299.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            11808..12296
                     /locus_tag="OO433_002893"
     CDS             11808..12296
                     /locus_tag="OO433_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6051300.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            12293..12832
                     /locus_tag="OO433_002894"
                     /pseudo
     CDS             12293..12832
                     /locus_tag="OO433_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT         3258 a         3089 c         3441 g         3164 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttcgtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgttgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgttgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgttgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatggttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgcgcgat ttaatgcccg taccgcctgc agtagcagaa
     2941 tattctaccg gcttgcggat gggtgacacc gcagagcaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgttc
     3361 ggtccggcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacaat gagcgatctg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgcttg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgtgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgacgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat cggcaactgc aaaacggtgc
     3961 aagaagcgga agctctggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg tggtgggctg gagttggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagcacaac attagagatg atcctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagtc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggttatgac gccgaagctc gggcgtttgg cgaactggcg atgacgccac
     4561 aatcgcaggc gctgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattgaaaca acagatggtg gcggaagttg agcaaaattg cgccgctcat accatctttg
     4981 cttcgaatac gtcatcttta ccgattggtg atatcgccgc tcacgccacg cgacctgagc
     5041 aagttatcgg cctgcatttc ttcagtccgg tggaaaaaat gccgctggtg gagattattc
     5101 ctcatgcggg tacatcagcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccaaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gtccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggtgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgacctgca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaatgggcg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag ccctcgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggtac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgtgttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaacctca ctactatcga agactctcct tatctataaa
     6661 taaaacactt tctacattca ttcggaaatt attcttctgc acgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagtt gaataatcag gcaggatgta attgaaactt gattaaattc tggctatagt
     6841 gtttatatat tatattgtca aaagtggata aatgccaatc gcattatata ttcattgttt
     6901 gttttttaag ctgttgatat tcattattga tatactttct attttttcta aatacttatt
     6961 ccgtattttt taaggatgtc cttaagataa tctttcttct gtcaatatat taattaaatc
     7021 tacatttact ctttagtaaa attaaattgc atcaggggtg tggaaacatc actatgcggc
     7081 cccacaccaa cgaatacaat tttctacgac ctgttctttt tggatcgcat gccgtattca
     7141 atggctgggt ataaacattg gctattgact gaatcaatgt attgcaatgt ttatattttt
     7201 aattatttat agtttaagga caaataatga gtaagtttgt taaaacagct attgctgcgg
     7261 caatggtgat gggcgtgttc acctctacgg caacaatcgc tgcgggtaac aacggtactg
     7321 cacgttttta cggcaccatt gaagactcag tgtgttctat cgttccggac gatcacaaac
     7381 tggaagttga tatgggtgat atcggtgccg aaaaactgaa aaataacggc accaccacgc
     7441 cgaaaagttt ccagattcgt ctgcaagatt gcgtatttga tacttaggaa acaatgacca
     7501 ctacctttac tggtaccgtt tcttctgcaa atagcggcaa ttattacacc attttcaata
     7561 ccgataccgg tgcggcattt aacaatgtta gcctggcgat cggtgactct ctgggtacct
     7621 cttacaaaag cggcatgggt attgaccaga aaatagtgaa agacacttct actaataaag
     7681 gcaaagcgaa gcagacactg aactttaaag cctggctggt cggcgcagct gatgcgccag
     7741 atctgggtaa tttcgaagca aataccacct tccagattac ttacctgtaa ggcatacccc
     7801 catagcgaca ctgctatggg ggatttaaat ggattttgac cgaacaagaa taaaaacgga
     7861 tagaaacgtg tatgcctgac cattctcttt ttcgattacg gattcttccg tggtgcattg
     7921 cgctggcaat gtcagggagt tatagcagtg tctgggctga agacgacatt cagtttgatt
     7981 cccgttttct ggaattaaaa ggcgacacaa aaattgatct gaagcgtttt tccagccagg
     8041 gatatgttga gcccggaaaa tacaatttac aggttcaact aaataaacag ccattggcgg
     8101 aagagtacga tatttactgg tatgctggtg aagatgacgc gagcaaaagc tatgcttgtc
     8161 tgacaccgga actggtagcg cagtttggtt taaaagaaga cgtggcgaaa aatctgcaat
     8221 ggagccacga tgctaaatgc ctgaaatccg gtcaactgga aggcatggaa attaaggctg
     8281 atttaagcca gtccgcatta gtcatttcac tgccacaggc ttacctcgaa tatacttatc
     8341 ccgactggga tccgccttca cgttgggatg acggcatctc cgggatcgtc gcggactaca
     8401 gcatcaacgc acaaacccgg cacgaagaaa atggcggtga tgatagtaac gagatcagcg
     8461 gcaacgggac ggtcggggtt aacctggggc cgtggcgtat gcgtgctgac tggcagacta
     8521 actatcaaca tactcgcagt aatgatgacg atgaattcag cggcgatgaa actcaaaaaa
     8581 aatgggagtg gagtcgctac tatgcctggc gggcgttacc atcattaaaa gccaaactgg
     8641 cgctgggcga ggattacctc agatccgata tttttgatgg ttttaactat gttggtggta
     8701 gtgtcagtac tgacgatcaa atgttgcctc ccaatctgcg cggctacgcg ccagacattt
     8761 ccggcgtggc acacaccaca gcaaaagtga ccgtcagcca gatggggcgt gtgatttacg
     8821 aaacgcaggt tccggctgga ccgtttcgta ttcaggatct tggtgattct gtctccggta
     8881 cgttgcatat tcgcattgaa gaacagaacg gccaggtgca ggaatatgac atcagcaccg
     8941 cctcgatgcc atacctcact cgtccaggtc agattcgcta taagatcatg atgggccgtc
     9001 cgcaagagtg gggacaccat gtcgagggtg aatttttttc tggtgctgaa gcttcctggg
     9061 ggatcgctaa cggctggtcg ttatatggcg gcgcactggg agatgaaaac tatcagtctg
     9121 cggcgcttgg cgtcggtcgc gatttgtcta cattcggcgc ggtcgcgttt gatgttactc
     9181 actcgcacac caaactggat aaagacaccg cttatggcaa aggttcgctg gacggtaact
     9241 ccttccgtgt gagttattcc aaagactttg accagctcaa cagccgcgtt acttttgctg
     9301 gatatcgctt ctcggaagag aactttatga ccatgagcga gtatctggat gccagtgaca
     9361 gcggaatggt acgcacgggc aacgacaaag agatgtacac cgccacttat aaccagaact
     9421 tccgcgatgc gggtgtttcg gtttatctca actatacccg ccatacctac tgggatcgcg
     9481 aggagcagat aaactacaac atcatgctct cgcactattt caatatgggc agtattcgta
     9541 atgtcagcat ctcgatgact ggctaccgtt acgagtatga caaccaggcc gacaaaggca
     9601 tgtacatttc gctcagtatg ccgtggggcg acaacagtac cgttagctat aacggtaact
     9661 atggcagtgg gacggacagc agtcaggtcg gttatttcag ccgtgtcgat gacgcgactc
     9721 actatcagtt gaacgtcggc accagtgaca aacacaccag cgttgacggc tattacagcc
     9781 atgatggttc gctggcgcag gttgacctca gtgcgaacta ccatgaaggg caatacacct
     9841 ctgcgggctt gtcgttacag ggcggcgcaa cgcttactgc ccacggtggc gcacttcacc
     9901 gtacccagaa tatgggcggg acacgcttgt tgattgatgc cgatggcgtt gccgatgttc
     9961 cggtggaagg taacggggct gctgtttata ccaatatgtt tggtaaagcc gtcgtttctg
    10021 acgtcaataa ctattaccgc aatcaggcgt atatcgacct caacagattg cctgaaaacg
    10081 ctgaagcaac ccagtcggtg gtgcaagcca cgctaactga aggtgccatt ggctaccgta
    10141 aatttgctgt cattagcgga caaaaagcga tggcggtgct gcgcctgagc gacggcagcc
    10201 atcctccgtt tggcgcagaa gtaaaaaatg ataacgagca gacagtgggc cttgtcgatg
    10261 atgacggcaa tgtttatctg gctggggtga aacctggcga acacatgagt gtgttctgga
    10321 gtggtgttgc gcattgcgat atcaacctgc cggacccgct gcctgccgat ctgtttaacg
    10381 gcttgttact gccatgccag cataaaggca atgtagcacc tatcacttcg ccggcggtca
    10441 aaccggcgat tcaggaacag acacagcggg tgacgccaac ggaacctccg acttcaattt
    10501 cagtaaacca gtaacgtgat taaggaatga tccatgttta atctgactaa cactgcaaaa
    10561 atcgttgtcc cggcactggc gctgctggcg acagcggtca gtttctccag ccacgcttcg
    10621 gtgacgcccg acaggacacg gctggtgttt aacgaaagcg acaagtccat cagcgtcacg
    10681 ctgcgcaaca acgatcccac actgccttat ctggcgcaga gctggataga ggatgcaaag
    10741 ggcaacaaaa tcagctctcc gcttgcggtg cttccgccag tgcagcgcat cgatgcgatg
    10801 atgaacggcc aggtgaaggt gcaggggctg ccggatatca acaaactgcc ggccgaccgc
    10861 gagagcgtgt tctatttcaa cgtgcgcgaa attccgccga aatcaaataa accgaacaca
    10921 ctgcagattg cgctgcagac gcgtatcaaa ctgttctggc gaccgaaggc gctggagaac
    10981 accagcatga aaagtccgtg gcagcataag gtgacgctca tccgtaacgg ccaggacttc
    11041 accgtcaata acccgacgcc gtactacgtg atcatcagca acgccagcgc taaaaaaggc
    11101 ggcaacccgc cggcgggatt cacaccgctg atgatggagc cgaaaacctc cgcaccgctg
    11161 aatgcgaaga tggacagcgt gccggtgctg acttacatca acgactacgg cgcgcggatg
    11221 ccgctgtact tcaaatgcac cggcaacagc tgccaggtgg atgaagagca gagccgcaaa
    11281 ggctgatgcc gggagggact tatgagtcac aagtttgtag cgggcgcagg gttgctggtg
    11341 ctggccggat ggggcatcag cggtttggct gttgctggca gtgatatcaa acaggtggat
    11401 ctgacgctgc gaatcctagt cgatggaccg ccgccttgct ccgtgaaggg aagcgctgtg
    11461 aaatttggca atgtgataat caacaggatc gatggtacca attaccaaca ggatgcgaaa
    11521 tacaccctca actgcggtaa taaagtcagc gatgacttac ggatgcagct caaaggcaat
    11581 accgccgtga tcaacggaga aacggttctc agcacggata tcgccggcct gggggtgcgg
    11641 atagaaaacg ccgccgacaa ctccctgttt gcagttggtg aaaataactg gacgcctttt
    11701 aatgtcaaca ctcagcctca gctgaaagcg gtgccggtaa aacagagcgg ggtgcaactg
    11761 actccgtcgg agtttaacgc cacgatgacc atggtggtgg attaccaatg aaaaaattcc
    11821 tgctcctttt actgtgcgct ggttgcgggg ctgtgctggc ggcggaagat gacatcacat
    11881 tccacggtac gctgatgtcg ccgcccgcct gtagcatcag cggcgggcaa accatcgaag
    11941 tgcaatttcg cgatctcatt atcgacagca ttgatggcaa ttatggtcgt cagaaagtgg
    12001 actacgaact gagctgcgac tcggatgtcc gcgatcccga gtggaatatg acactctcct
    12061 ggacggggac tgccacctcc tacaacgact ctgctattga gacggatgta ccgggcttcg
    12121 gaattgagct tcagcatgac ggtcagcgtt ttaagctcaa cgagccgctt tccattaacg
    12181 ccaccgattt tagccagaag ccaaagctgg aggcggtacc ggtcaaagcg gctgatgcgg
    12241 tgctgacgga cacgaccttt tctgcttacg caaccttacg ggtggattac caatgaaaac
    12301 agcacacatt tattcacccg gttatgcgct gttagcaggc ctgctgctga ccgccagcac
    12361 aggagcacag gcagtcgaca acaacctgca tttttacggc aacctgctga gtaaatcctg
    12421 cacgctggtg gtggatggtg ccaacctggc agaaatccat tttcccaccg tcagcaaccg
    12481 ggatttgatg gtatctgggc agtccgcgca tctgccggtt gtgtttaagc tgaaagactg
    12541 caaagggccg gcgggataca acgtcaaagt cacgctgagc ggcaccgaag acagcggtca
    12601 gccgggtttt ctggcagtcg atgacacctc caccgcgcag ggcgtgggga tagggatgga
    12661 aacgctcgac ggcaagcggg tggatattaa caacaccacc ggggcgacct ttgcgcttac
    12721 caacggcaac aatgatatta atttccgggc ctggatctag gcgaaaagcg gtcgtgatgt
    12781 aaccatcggt gatttcaccg ccagcctgac agcaactttt gagtatattt aaaatgaaaa
    12841 tccttcgtca tgttttatgt cttttattgc tgttaatgtt caatacatgc ggccaggcgg
    12901 cgacgaaagt tggtgagtta tatgtgccag ctggtgccag ttcacctgcg ac
//
LOCUS       DAJTSL010000113        12942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTSL010000113 DAJTSL010000000
VERSION     DAJTSL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.113"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            21..995
                     /gene="cysB"
                     /locus_tag="OO433_002895"
     CDS             21..995
                     /gene="cysB"
                     /locus_tag="OO433_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6051301.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QTRYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1290..1454
                     /locus_tag="OO433_002896"
     CDS             1290..1454
                     /locus_tag="OO433_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6051302.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1457..1624
                     /locus_tag="OO433_002897"
     CDS             1457..1624
                     /locus_tag="OO433_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051303.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1738..1833
                     /locus_tag="OO433_002898"
     CDS             1738..1833
                     /locus_tag="OO433_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051304.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            1997..4672
                     /gene="acnA"
                     /locus_tag="OO433_002899"
                     /pseudo
     CDS             1997..4672
                     /gene="acnA"
                     /locus_tag="OO433_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
     gene            complement(4736..5326)
                     /gene="ribA"
                     /locus_tag="OO433_002900"
     CDS             complement(4736..5326)
                     /gene="ribA"
                     /locus_tag="OO433_002900"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6051305.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5496..6260
                     /gene="pgpB"
                     /locus_tag="OO433_002901"
     CDS             5496..6260
                     /gene="pgpB"
                     /locus_tag="OO433_002901"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6051306.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLLWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6409..6717
                     /gene="lapA"
                     /locus_tag="OO433_002902"
     CDS             6409..6717
                     /gene="lapA"
                     /locus_tag="OO433_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6051307.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            6724..7893
                     /gene="lapB"
                     /locus_tag="OO433_002903"
     CDS             6724..7893
                     /gene="lapB"
                     /locus_tag="OO433_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6051308.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNRARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8086..8823
                     /gene="pyrF"
                     /locus_tag="OO433_002904"
     CDS             8086..8823
                     /gene="pyrF"
                     /locus_tag="OO433_002904"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6051309.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8823..9149
                     /gene="yciH"
                     /locus_tag="OO433_002905"
     CDS             8823..9149
                     /gene="yciH"
                     /locus_tag="OO433_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6051310.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9275..9493)
                     /gene="osmB"
                     /locus_tag="OO433_002906"
     CDS             complement(9275..9493)
                     /gene="osmB"
                     /locus_tag="OO433_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6051311.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9762..10511)
                     /locus_tag="OO433_002907"
     CDS             complement(9762..10511)
                     /locus_tag="OO433_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6051312.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEVIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(10601..10774)
                     /locus_tag="OO433_002908"
     CDS             complement(10601..10774)
                     /locus_tag="OO433_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051313.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(10921..12906)
                     /gene="pdeR"
                     /locus_tag="OO433_002909"
     CDS             complement(10921..12906)
                     /gene="pdeR"
                     /locus_tag="OO433_002909"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6051314.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKSEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         3291 a         3054 c         3266 g         3331 t
ORIGIN      
        1 gtctaagtgg atggtttaac atgaaattac aacaacttcg ctatattgtt gaggtggtca
       61 atcataacct gaatgtctca tcaacagcgg aaggacttta cacatcacaa cccgggatca
      121 gtaaacaagt cagaatgctg gaagacgagc taggcattca aattttttcc cgaagcggca
      181 agcacctgac gcaggtaacg ccagcagggc aagaaataat tcgtatcgct cgcgaagtcc
      241 tgtcgaaagt cgatgccata aaatcggttg ccggagagca cacctggccg gataaaggct
      301 cactgtatat cgccaccacg catacccaga cacgctacgc attaccaaac gtcatcaaag
      361 gctttattga gcgttatcct cgcgtttctt tgcatatgca ccagggctcg ccgacacaaa
      421 ttgctgatgc cgtctctaaa ggcaatgccg atttcgctat cgccacagaa gcgctgcatc
      481 tgtatgaaga tttagtgatg ttaccgtgct accactggaa tcgggctatt gtagtcactc
      541 cggatcaccc gctggcaggc aaaaaagcca ttaccattga agaactggcg caatatccgt
      601 tggtgacata taccttcggc tttaccggac gttcagaact ggatactgcc tttaaccgcg
      661 cagggttaac gccgcgtatc gttttcacgg caacggatgc tgacgtcatt aaaacttacg
      721 tccggttagg gctgggggta ggggtcattg ccagcatggc ggtggatccg gtcgccgatc
      781 cggaccttgt gcgcgttgat gctcacgata tcttcagcca cagtacaacc aaaattggtt
      841 ttcgccgtag tacttttttg cgcagttata tgtatgattt cattcagcgt tttgcaccgc
      901 atttaacgcg tgatgtcgtt gatgcggctg tcgcattgcg ctctaatgaa gaaattgagg
      961 tcatgtttaa agatataaaa ctgccggaaa aataatttct tgcgttattt tcggcacctt
     1021 ttatgtagcg aaggtgccgg aatatattct cttttgttac ttatttttat accgtgcaca
     1081 atatttttat ccctcatatt tatagggtaa attacctgta aagtagtgtc taattacccg
     1141 cctcgcaaat tccagttttc aaatatctat ttcaggtcaa aagattgaat tattcactgg
     1201 agacgattcg taaattcgct ggatttttga ctaaagtttc tttaggattt gtctcatcgt
     1261 atgattaatt acaccaatcg ttcaacgtct tgctgataag tgatggcgat tatatgaggt
     1321 tagcaatgcc ttctggaaat caggaacccc gccgcgatcc tgaattaaaa cgtaaagcct
     1381 ggctggcggt ttttcttggt tctgcacttt tctgggtggt tgtcgcactg ctgatttgga
     1441 aagtgtgggg ataactatgg tcggtcagga gcaactggag tcgtcaccat tatgccagca
     1501 tagtgacaat gaaacagaaa cgaaacggga atgttccgtc gttattccag acgactggca
     1561 actaacatcg cagcagcaag cctttataga actgtttgct gaagatgatc agccgaaaca
     1621 ataattatca tcattcttat tacccatttt taatgaatta aagggctttt aatacaccgc
     1681 agcaataaca gcttgagtta tctcaacaca aaataataac cgttaagggt gtagcctatg
     1741 atcaacacaa atatgaaata ttggtcctgg atgggcgcgt tttctctgtc gatgctcttc
     1801 tgggccgaac tcctctggat cattactcac tgatccttga ccccgctgcg gcggggttgt
     1861 catttgcttt gccacaaggt ttctcctctt ttatcaattt gggttgttat caaatcgtta
     1921 cgcgatgttt gtgttatctt taatattcac cctgaagaga atcagggctt cgcaaccctg
     1981 tcattaagga ggagctatgt cgtcaaccct acgagaagcc agtaaggaca cgttgcaggc
     2041 caaagataaa acttaccact actacagcct gccgcttgct gctaaatcac tgggcgatat
     2101 cacccgtcta cccaagtcac tcaaagtttt gctcgaaaac ctgctgcgct ggcaggatgg
     2161 taactcggtt accgaagagg atatccacgc gctggcagga tggctgaaaa atgcccatgc
     2221 tgaccgtgaa attgcctacc gcccggcaag ggtgctgatg taggacttta ccggcgtacc
     2281 tgccgttgtt gatctggcgg caatgcgcga agcggttaaa cgcctcggcg gcgatactgc
     2341 aaaggttaac ccgctctcac cggtcgacct ggtcattgac cactcggtga ccgtcgatcg
     2401 ttttggtgat gatgaggcat ttgaagaaaa cgtacgcctg gaaatggagc gcaaccacga
     2461 acgttatgtg ttcctgaaat ggggaaagca agcgttcagt cggtttagcg tcgtgccgcc
     2521 aggcacaggc atttgccatc aggttaacct cgaatatctc ggcaaagcag tgtggagtga
     2581 attgcaggac ggtgaatgga ttgcttatcc ggatacactc gttggtactg actcgcacac
     2641 cactatgatc aacggccttg gcgtgctggg gtggggcgtg ggtgggatcg aagcagaagc
     2701 cgcaatgtta ggccagccgg tttccatgct tatcccggac gtagtgggct ttaaacttac
     2761 cggtaaatta cgtgaaggta ttaccgccac agacctggtt ctcactgtta cccaaatgct
     2821 gcgcaaacat ggcgtggtgg ggaaattcgt cgaattttat ggtgatggtc tggattcact
     2881 accgctggcg gatcgcgcca ccattgccaa tatgtcgcca gaatatggtg ccacctgtgg
     2941 cttcttccca atcgatgctg taaccctcga ttacatgcgt ttaagcgggc gcagcgaaga
     3001 tcaggttgag ttggtcgaaa aatatgccaa agcgcagggc atgtggcgta acccgggcga
     3061 tgaaccgatt tttaccagta cgttagaact ggatatgaat gacgttgaag cgagcctggc
     3121 agggccaaaa cgtccacagg atcgcgttgc actgcccgat gtaccaaaag catttgccgc
     3181 cagtaacgaa ctggaagtga atgccacgca taaagatcgc cagccggtcg attatgttat
     3241 gaacggacat cagtatcagt tacctgatgg cgctgtggtc attgctgcga taacctcgtg
     3301 caccaacacc tctaacccaa gtgtgctgat ggccgcaggc ttgctggcga aaaaagccgt
     3361 aactctgggc ctcaagcggc aaccgtgggt caaagcgtcg ctggcaccgg gttcgaaagt
     3421 cgtttctgat tatctggcaa aagcgaaact gacaccgtat ctcgacgaac tggggtttaa
     3481 ccttgtggga tacggttgta ccacctgtat tggtaactct gggccgctgc ccgatcctat
     3541 cgaaacggca atcaaaaaag gcgatttaac cgtcggtgcg gtgctgtccg gcaaccgtaa
     3601 ctttgaaggc cgtatccatc cgctggttaa aactaactgg ctggcctcgc cgccgctggt
     3661 ggttgcctat gcgctggcgg gaaatatgaa tatcaacctg gctgctgagc ctattggtca
     3721 tgatcgtaaa ggcgacccgg tttatctgaa agatatctgg ccatcggcac aggaaattgc
     3781 ccgtgcggta gaacaagtct ccacagaaat gttccgtaaa gagtacgcag aagtttttga
     3841 aggcacagca gagtggaagg aaattaacgt cacacgatcc gatacctacg gttggcagga
     3901 ggattcaacc tatattcgct tatcgccttt ctttgatgaa atgcaggcaa caccagcacc
     3961 tgtggaagat attcacggtg cgcggatcct cgcaatgctg ggggattcag tcaccactga
     4021 ccatatctct ccggcgggca gtattaagcc cgacagcccg gcgggtcgat atctacaagg
     4081 tcggggtgtt gagcgaaaag actttaactc ctacggttcg cggcgtggta accatgaagt
     4141 gatgatgcgc ggcaccttcg ccaatattcg catccgtaat gaaatggtgc ctggcgttga
     4201 aggggggatg acgcggcatt tacctgacag cgatgttgtc tctatctatg atgccgcgat
     4261 gcgctataag caggagcaaa cgccgctggc ggtgattgcc gggaaagagt atggatcagg
     4321 ctccagccgt gactggacgg caaaaggtcc gcgtctgctt ggtattcgtg tggtgattgc
     4381 cgaatcgttt gaacgaattc accgttcgaa tttaattggc atgggcatct tgccgctgga
     4441 atttccgcaa ggcgtgacgc gtaaaacgtt agggctaacc ggggaagaga agattgatat
     4501 tggcgacctg caaaatctac aacccggcgc gacggttccg gtgacgctta cgcgcgcgga
     4561 tggtagccag gaagtcgtac cctgccgttg tcgtatcgac accgcgacgg agttgaccta
     4621 ctaccagaac gacggcattt tgcattatgt cattcgtaat atgctgaagt aacaagtatt
     4681 tgcttgccgg ttattttgct tccggcaagc aaattaattc cacaatgcga gagggttatt
     4741 tgttcagcaa atgacccatt ttctcggctt tggtatcgag ataatgttcg ttattggggt
     4801 tacgacctac aatcaatggt acgcgttcaa caatattaat ccctgcttcg gtcagaattt
     4861 cgactttttt cgggttattg gttaacaagc ggacttcatt gacgccaagg agtttgaaca
     4921 tatcagcgca aagagtgaag tcgcgctcat cagcggcgaa gcctaactgg tggttagcct
     4981 ctacggtatc gtaaccttga tcctgcagtg cgtaagcgcg gattttattc agcagaccaa
     5041 tgttacgacc ttcctgacgg tgatacagca aaataccacg gccttcctcg gcaatttgcg
     5101 tcaatgccgc ttcgagctgg aagccacaat cgcagcgcaa gctgaacagg gcgtcaccgg
     5161 tcagacattc ggaatggacg cgcgcaagta ccggggtatg cccggaaata tcgccataga
     5221 ctagcgcgac atgatcgtgt ccggttgcca gttcttcaaa tcccaccatc aggaaatcgc
     5281 cccatggggt tggcagtttg gcttctgcca cacgtttaag ctgcatgaaa ttctccagat
     5341 aatgctggtt ctgtattggc ttattttgcc ataacgagaa ggggttcacc taatcacgac
     5401 gcgtcgatcg ttcacggaat ggcacaaatc tgtcaatttt tcctggaact ggcgttttca
     5461 gttatgattg tgggacttat caaaaaggag aggccatgcg ttcgattgcc agacgtaccg
     5521 cagtgggagc tgcactattg cttgtcatgc cagtagccgt atggatttct ggctggcgtt
     5581 ggcaacctgg agaacaaagt tggctactaa aagcggcttt ttgggttact gaaactgtca
     5641 cccagccctg gggcgtcatt acacatttga ttttattcgg ctggtttctc tggtgtctgc
     5701 gttttcgcat taaggctgcc tttgtattat ttgccattct ggcggccgca atccttgtgg
     5761 gacaaggcgt taaatcctgg atcaaagaca aagtccagga accacgacct tttgttatct
     5821 ggctggaaaa aacacatcat attccggttg atgagttcta cactttaaag cgagcagaac
     5881 gcggaaatct agtgaaagaa cagttggctg aagagaaaaa tatcccacaa tatttgcgtt
     5941 cacactggca gaaagagacg gggtttgcct ttccttccgg tcacacgatg tttgctgcca
     6001 gttgggcact gctggccgtt ggtttgctgt ggccgcgtcg gcgaacgtta accattgcta
     6061 tcttgctgct ctgggcaacg ggagtcatgg gaagccgcct gctgctcggg atgcattggc
     6121 cacgcgatct ggtagtagct acattgattt cgtgggcgct ggtggcggtg gcaacgtggc
     6181 ttgcgcaacg aatttgtggg ccattaacac cacctgcgga agaaaatcgc gaaatagcgc
     6241 aacgagaaca agaaagttaa cgctggttga ttttccgaat ttagccctta aatcatcaac
     6301 aatgcgcgtg gatgccattt cgcagacggc gcgaaaatgg tactttaaag ggctattgcg
     6361 gtaagttgac cataatttat tcgctctaac cacataacgg gaagtaatgt gaaatattta
     6421 ctcattttct tactggtgtt agcgatcttc gtgatttcgg tcacgttggg tgcgcagaac
     6481 gatcaacagg tgacgtttaa ttatctgtta gcgcaagggg agtaccgtat ttccacattg
     6541 ctggcggtat tgtttgctgc ggggtttgct atcggttggt tgatttgtgg cctgttctgg
     6601 ctgcgagttc gtgtttccct ggcgcgcgct gaacgtaaaa taaagcgact ggaaaaccag
     6661 ctttcacccg cgactgacgt ggctgtagtg ccgcactcgt cagcggcgaa ggaataactt
     6721 tctatgctgg agttgttgtt tctgcttttg cctgtagccg ctgcctatgg ctggtatatg
     6781 ggccgcagaa gtgcgcaaca aaacaagcaa gatgaagcca accgcttgtc gcgtgattac
     6841 gtagcggggg ttaacttcct gcttagtaat caacaggata aagcggtaga cctgtttctc
     6901 gatatgctta aagaggatac aggcaccgtt gaagcccacc ttacgctcgg aaacctgttc
     6961 cgttcgcgtg gcgaagttga tcgcgctatt cgcatccatc agaccctaat ggaaagcgcc
     7021 tcgctgacct atgaacagcg tctgttggcg attcaacaac tagggcgtga ttacatggcc
     7081 gccgggttat acgaccgcgc ggaagacatg ttcaatcagc tgaccgatga aactgacttc
     7141 cgcattggcg ctctgcaaca gttgctacaa atctaccagg ctaccagtga gtggcagaaa
     7201 gcaattgatg ttgccgaacg cctggtgaag ctgggtaaag ataaacagcg cgtcgaaatt
     7261 gcccatttct actgtgagtt agccctgcag catatggcca gcgacgatct cgatcgtgcc
     7321 atgaccttgc taaaaaaagg ggcggcggca gataaaaaca gagcccgcgt atccatcatg
     7381 atgggacgcg tgtttatggc gaaaggagaa tacgccaaag ccgtcgaaag tttgcaacgc
     7441 gtgatatccc aggacagaga actggtcagc gaaacgctgg aaatgttgca aacctgctat
     7501 cagcagttgg gtaaaactgc cgaatgggca gaattcctgc aacgcgcggt ggaagagaac
     7561 accggtgccg atgctgaatt gatgcttgct gatatcatcg aagcgcgcga cggtagtgag
     7621 gccgcacagg tctatattac gcgccagctt cagcgtcatc cgaccatgcg tgtgttccat
     7681 aagttaatgg attaccactt aaatgaagcg gaagaagggc gtgccaaaga gagcctgatg
     7741 gtgctgcgtg acatggttgg cgaaaaggta cgtagtaagc ctcgttatcg ctgccagaaa
     7801 tgtggtttta ccgcatacac cctctactgg cattgtccgt cttgtcgggc ctggtcaacc
     7861 attaaaccga ttcgcggtct tgatggcctg taatttttaa aaaaatccga ctttagttac
     7921 aacatgctag ttattgatgt tccattgtgc tccggcaacg acggagcaga aaaagcctgc
     7981 caggggaaaa atcgcaactg ttaatttttt atttccacgg gtagaatgct cgccgtttac
     8041 ctgtttcgcg ccacttccgg tgcccatcat caagaaggtc tggtcatgac gttaactgct
     8101 tcatcttctt cccgcgctgt tacgaattct cctgtggttg ttgcccttga ttatcataat
     8161 cgtgatgacg cgctgtcctt tgtcgacaag atcgacccac gcgattgtcg tctgaaggtc
     8221 ggcaaagaga tgtttacatt gtttgggcca cagtttgtgc gcgaacttca acagcgtggt
     8281 tttgatatct ttcttgacct gaaattccac gatattccta acactgcagc gcacgctgtc
     8341 gctgctgcag ctgacttagg cgtgtggatg gtgaatgttc atgcctctgg tggggcgcgt
     8401 atgatgaccg cagcgcgtga ggcactggtt ccgtttggca aagatgcacc gcttttgatt
     8461 gctgtaacag tgttgaccag catggaagct agcgacctgg ccgatcttgg cgtgacactg
     8521 tcacctgcag attatgcaga acgtctggcg gcactgacgc aaaaatgtgg ccttgatggt
     8581 gtggtgtgtt ctgctcagga agctgtgcgc tttaaacagg tattcggtca ggagttcaaa
     8641 ctggttacac cgggcattcg tccgcagggg agtgatgctg gtgaccagcg ccgcattatg
     8701 acgccagaac aggcgttggc ggctggtgtt gattatatgg tgattggtcg cccggtaacg
     8761 caatcggtag atccagcgca aacactgaaa gcgatcaacg cctctttaca gcggagtgca
     8821 tgatgagtga ttccaacagc cgtctggtct actcaacgga aaccggacgt attgatgagc
     8881 caaaagcggc cccagtacgc cctaaaggcg atggtgtggt gcgtattcag cgtcagacca
     8941 gtggacgtaa aggtaagggc gtttgcctga ttaccggtat cgatctcgat gatgccgaac
     9001 tgacaaaact tgcagcggaa ctgaagaaaa aatgcggctg cggcggagca gttaaagatg
     9061 gagttattga aatccagggc gataagcgtg atttattaaa atcactactt gaagcgaaag
     9121 ggatgaaggt aaaactcgca ggcggttaac ataaaaagcc acggatatat ccgtggcttt
     9181 cgaatatttt actgtgtgta ttattcagtt atttcgtact gaacaaaatc aagcgtatta
     9241 taattgcgag tcgataatcg acatttattt acgattattt accgacctgg tgaccaataa
     9301 caccaccgac agctgcacca cctaatgtac ccaacgtact gccatcggtc agtactgcac
     9361 cgcctaatgc ccctgcaccc gcgccgattg cggtgttgcg gtcccgttta gaccagttag
     9421 aacaggcact cagagacatt gccaaagtaa ttgccagaac agccgcggtc atttttttgc
     9481 tcgttacaaa cataatactc tctcctgaat ttatgattca cggaagtaag ctctctataa
     9541 ctataatagc taagaataag cctggtgaaa ttattccgtg aaatctgctc gcgcgcaggg
     9601 ttatatcacg caggtgataa tgacttcctg ttatatcgct gataataatt ttatatctta
     9661 agcgtgttaa taacaggtaa atagtcttaa ttatcaacca ggaatcatct tagagcggat
     9721 gatttgccaa actgcaaatc atccgtagag aagggaaatg gttaaatgtc aacgacgtga
     9781 acggtgagtt tactgtgctc aagatgcatc agatcgctgg cgcgtatttt cgaatcggta
     9841 atcacgcgat tgaatcgctc aacgggaccg atggagtatg aatgtacagc accaaatttc
     9901 gagctgtcag tcaggactat cacttcgcac tctttttcca gcacggcatt gaccacatcg
     9961 gtacgcatca tatcgcgacc ggtgaatcca gtttcaggtt gccagccatc aataccaata
    10021 aatgctttgc tgaaatgcac ctgctggatg cactggcgtg tcaacgggcc aaccatactt
    10081 tcgctttttt tctggtacac gccaccgagc aaaatgactt cacaaggcgc gtctttcagc
    10141 aaatgcgcga tgtagctgct gaccgtgatg atagtgacat ttttcttctg ctcccccaga
    10201 gtccgggcga gcagggcatt gctgctgcca ttttcgataa agatggtttc gcccggttga
    10261 accagtgacg cggcaaactc ggcgagttca cgcttcagcg tatagttgct catcatacgg
    10321 gtttcgacgt catcactatc aagcgaaact gcaaagccat gtgcacggcg gaggtaactc
    10381 agtttttcga gggtgttgag atcctggcga atggtaactt cagaaactcc agtggctttt
    10441 gccagatcgg ttacgctaac ctgaccctgg tcaatgacca tctgtagaat agtttgttgt
    10501 cgggaattca tcgctgtaat tcttataacg ttataatact aaataaaaaa atactgacgc
    10561 gccgggcatc ctcaaatagg aaaacctgaa ggcacgattg ttaaatctcc cacggtgcct
    10621 tcgggatgtc tggcgtagat tcagcaacct ggcgcaagag ttctgcttta agtatctcga
    10681 gattatggat cgcagagtgg taatcgcctg ccacaaggat atctaacaca atatcaatac
    10741 gttcggcaac gcgctgggca tcaaattcag acatcaagga catccttata gcaaaagtgg
    10801 aaaatgaata atgcaaaaat tgccgctaaa agagaagtgt ttaacagcaa cggctaatta
    10861 tcatccagga atacgatata taatgacggg atatagcgct aagtatatat attcatctac
    10921 ttatgcgcgc ttcagatagc gtttatacca gcgttcgaag gcgacggcgg gcatcggttt
    10981 ggcaaacaaa aatccttgcc gctcattgat cccgttcttg gttaaaaaag catcttcctt
    11041 tgcactctct acaccttcgg cgatcacctg aagattcaat gcctgggcca cagcgacgat
    11101 cgcccggacc agtgactgcg agacaggttg tttgtgaata tctcgaacaa aaacctggtc
    11161 aagtttgatg gcatcgatcg gaaagcgcgc cagttgcgaa agtgaagagt agccggtacc
    11221 aaaatcatcc agatgcactt gcgcacctag ttggctaaat tgttgaataa cagacagtgc
    11281 cagttcatca ttctcaatca gacaactctc tgtcagttca acatctatag ggcagtattc
    11341 aaaattgagt tcctggagaa cctgtttcag ggcggtgaaa atggtttgat cggcgagctg
    11401 acgtgcagaa atatttaccg ccactcgcag gtttatgcct ttatcccgcc actttgccac
    11461 ctggcgtacg acatcgagaa tcacccaacg gcctaaaggc acaattagcc ctgactcttc
    11521 ggcgtaggaa atgaagtcca acggtggaat caacccacgt tcaggtgact gccaacgtac
    11581 tagtgcttcc agactgcgca cttcgccacg ccaggtgatt ttcggttgat agtgaataac
    11641 caactgatcg ttttccagtg ctttacgcaa gttggtatcc agccagagat attcaaatac
    11701 ccgttgattc atttctgggg taaaaacgca aaattgtcct cgtccgcctt ccttcgctgt
    11761 gtacattgct gtgtcggcgt gacgaataat agccgtgctg tctgaaccat gttcgggaga
    11821 gagtgcaata cctactgaac agctggtata aacttcaatt aaaccaatgc gaaaggggag
    11881 ccgtaagcgg gtcaaaattc gtgatgccat tgcttccagc gcgctttgtg aggtgttgga
    11941 tgccagtacc agaaactcat ccccacctgg acgcgccaac acctggtcat gttcgagaca
    12001 gcttaaaata gccaatgaca cgtcgcgtaa taactggtca ccaaacaaat gcccataggc
    12061 gtcgttgacc tttttgaaat tatccaaatc aagatacaca accccaactt tattgttatc
    12121 tgcatgatta atagcgtgat cgattaaatc ctgcattgcg ttacgattcg gcagtccggt
    12181 gatactgtcg gtatttgcca gaatacgcag tcgctcctga gcgcggcgct cttcggtaat
    12241 gtcggtgccg gaacagatta aaaaaatctc gcttttgcca ctgccgctgt ggacaaattt
    12301 attgcgaaac agaaacagcc gctggccttt acatgttggt atccacagtt cgacttcata
    12361 tgcattgccg cttcgaaaaa atacacggtt attgcgcctg gatgccgcag cttcacgacg
    12421 gctcataaac agtttaaaca cgctttggcc aatgacgtcg tgttctttca acccagtgta
    12481 atcttcacat aaccgattga agcgttggat attcccccgg ctgtcgagaa tcactattgc
    12541 ggaatgtgct tcagaaacta cctgctcagc aaaggaaagc ccgtgtgata aatcacgagc
    12601 aactgccggt gtgtcatgcc atgcagacgc gttgccagcc cattcccgtt tattgatttt
    12661 tcgtcctacc agatgcacag aaagatcgct ctcatcgacg gtcagactca tcatcaggct
    12721 ggaggtgatg accgtcattt ccctgatgcg agcagcctgt tcggcagata actgcaaagt
    12781 acgatcaggt tctgtggttt cggtggtaga aaagcgcaaa acatcgctgc tttccgtcaa
    12841 ccgccagtat ggattgtgcg atccgagaaa gttgtacaac gttgtggact ccctaacggt
    12901 tttcatgatg catatcctcc ggttaaataa aaggaacaac at
//
LOCUS       DAJTSL010000114        12887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTSL010000114 DAJTSL010000000
VERSION     DAJTSL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.114"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            117..932
                     /gene="mepH"
                     /locus_tag="OO433_002910"
     CDS             117..932
                     /gene="mepH"
                     /locus_tag="OO433_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6051315.1"
                     /translation="MAQINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1060..1641
                     /gene="sodB"
                     /locus_tag="OO433_002911"
     CDS             1060..1641
                     /gene="sodB"
                     /locus_tag="OO433_002911"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6051316.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1787..2956)
                     /locus_tag="OO433_002912"
     CDS             complement(1787..2956)
                     /locus_tag="OO433_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6051317.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVEPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYPGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAAAFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3122..3211)
                     /gene="ynhF"
                     /locus_tag="OO433_002913"
     CDS             complement(3122..3211)
                     /gene="ynhF"
                     /locus_tag="OO433_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6051318.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3510..4535
                     /gene="purR"
                     /locus_tag="OO433_002914"
     CDS             3510..4535
                     /gene="purR"
                     /locus_tag="OO433_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6051319.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4532..5464)
                     /locus_tag="OO433_002915"
     CDS             complement(4532..5464)
                     /locus_tag="OO433_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6051320.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5577..6788
                     /locus_tag="OO433_002916"
     CDS             5577..6788
                     /locus_tag="OO433_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6051321.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKAGNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            7079..8227
                     /gene="cfa"
                     /locus_tag="OO433_002917"
     CDS             7079..8227
                     /gene="cfa"
                     /locus_tag="OO433_002917"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6051322.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSMVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8267..8908)
                     /gene="ribE"
                     /locus_tag="OO433_002918"
     CDS             complement(8267..8908)
                     /gene="ribE"
                     /locus_tag="OO433_002918"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6051323.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9123..10496
                     /gene="mdtK"
                     /locus_tag="OO433_002919"
     CDS             9123..10496
                     /gene="mdtK"
                     /locus_tag="OO433_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6051324.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(10537..11793)
                     /locus_tag="OO433_002920"
     CDS             complement(10537..11793)
                     /locus_tag="OO433_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6051325.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12101..12177
                     /locus_tag="OO433_002921"
     tRNA            12101..12177
                     /locus_tag="OO433_002921"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12135..12137,aa:Val,seq:gac)
     gene            12182..12258
                     /locus_tag="OO433_002922"
     tRNA            12182..12258
                     /locus_tag="OO433_002922"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12216..12218,aa:Val,seq:gac)
     gene            12366..12671
                     /locus_tag="OO433_002923"
     CDS             12366..12671
                     /locus_tag="OO433_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6051326.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12797..>12887
                     /locus_tag="OO433_002924"
     CDS             12797..>12887
                     /locus_tag="OO433_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR6051327.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSIS"
BASE COUNT         3159 a         3345 c         3058 g         3325 t
ORIGIN      
        1 aacgtatttt ttaacaataa aagctattaa ctttctcttc ttctatgcat tagaatcatc
       61 aagttttgta aatcagacgc aggcatgata gacctgcctt aacagaggga cgctcagtgg
      121 cgcagataaa ccgtatttcg atcacgctct gtgctttact ttttaccacc ctgcctttaa
      181 cgcctatggc ccatgcttca aagcaagcca gggagagttc tgctaccact catatcacca
      241 aaaaagcaga taaaaagaaa agcacggcaa ccaccaaaaa gacccagaaa acagcgaaaa
      301 aagccgccag taaaagtacg accaaaagca aaaccgcttc ttccgttaaa aaatcttcca
      361 ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa acggcctcag
      421 ccagctttac cgaaaagtgt accaaacgta agggctataa atcgcattgt gtgaaagtca
      481 aaaatgccgc gtcaggaact cttgccgacg cgcacaaagc gaaggtgcaa aaagctacga
      541 aagtggcaat gaataaactg atgcagcaaa ttggtaagcc atatcgttgg ggtggcagct
      601 caccgcgtac cggttttgat tgcagcggcc tggtttatta cgcttataaa gatttggtga
      661 aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct gcgtgatgca gcgccaatcg
      721 aacgtagtga actgaaaaac ggcgacctgg tctttttccg tactcaggga cgcggcacag
      781 ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg cgcacaggtc
      841 aggaaattca aatcacctct ctcagtgaag actactggca gcgccactat gttggcgctc
      901 gtcgggtaat gaccccaaaa acacttcgct aaaactttac cctgttgtta cggcaacagg
      961 gtaagttcat cttttgtctc accttttaat ttgctaccct atccatacgc acaataaggc
     1021 tattgtacgt atgcaaatta ataataaagg agagtagcaa tgtcattcga attacctgca
     1081 ctaccatatg ctaaagatgc tctggcaccg cacatttctg cggaaactat cgagtatcac
     1141 tacggcaagc atcatcagac ttatgtcact aacctgaaca acctgattaa aggtaccgcg
     1201 tttgaaggta aatcactgga agagattatt cgcagctctg aaggtggcgt attcaacaac
     1261 gcagctcagg tctggaacca tactttctac tggaactgcc tggcaccgaa cgccggtggc
     1321 gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt tgccgatttc
     1381 aaagcgcagt ttactgatgc agcgatcaaa aactttggtt ctggctggac ctggctggtg
     1441 aaaaacagcg atggcaaact ggctatcgtt tcaacctcta acgcgggtac tccgctgacc
     1501 accgatgcga ctccgctgct gaccgttgat gtctgggaac acgcttatta catcgactat
     1561 cgcaatgcac gtcctggcta tctggagcac ttctgggcgc tggtgaactg ggaattcgta
     1621 gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag cattgcctga tgcgcttcgt
     1681 ttaccatgcc aacgcggatg aggcgttaac agcgcattcg gcaagataaa tcgcacgttg
     1741 tcagcaactg taacgcagaa ggttatcctt ctgcgttttt gtttaattag ctgttagcaa
     1801 cgcaaactgt ttcaggttgt tttctggctg acataaacac cagcaataat gccagtcccg
     1861 cgacaatcgc tcccatcacc ggcacaaagc tgtatcccag cccagcggaa attaccgcac
     1921 caccagcagc tgctcccagc gcatttccaa gattaaaggc accaatattg actgatgaag
     1981 acagacctgg cgcttcactg gcgacacgca tcacgcgcat ctgtaacggc ggtacgaccg
     2041 caaaggctgc tgcgccccac accaccatgc taatagctgc gccgaactca ttgcgggcca
     2101 ggaacgggat tgccagcata atcaccatca acaacaacaa aaagcctttc aacgtgccgt
     2161 taactgaacg atctgccagt ttgccgccgg gatagttacc gatagagaat ccgacaccaa
     2221 tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt tgcagtaccg
     2281 gagagatata ggtgtagaga gtaaacattg caccagctcc cagtaccgtc gtcagcaatg
     2341 cagacaacac ctgcggacgc attaataccg ccagctcttt tttcacttca ggtcgtgccc
     2401 ctgcaccacc tttaggtaat gagaagaaca gacttaccat tgaaatcact cccagccccg
     2461 ccgttgccag aaatgacatc cgccagccga tggtttcacc caaccaggtc gccgccggca
     2521 cgccaccgat atttgccagg gttaacccca taaacatagt ggcaactgcg ctggcctgtt
     2581 tatgttttgg ctccacgctt gcggccacga ctgaacccaa accaaaaaat gctccgtgat
     2641 tcaggctggt caaaatgcgt gaaagcatca gggtcatata atccggcgcg atggcggaaa
     2701 gtacgttgcc gagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg cgggcacgat
     2761 gagaaagtag aagcgtcatc agcggcgcgc caaccattac gccaactgca taggcactga
     2821 ttaacattcc ggcagcggga atcgagacat ccacaccgcg cgcaatgacg ggcaacaagc
     2881 ccattggcga gaactccgtt gtcccgatac caaacgcgcc aatcgccaga gccagcaacg
     2941 gatagttaat tttcatgcct tatctccacc tcttcgcgtc attacgcgat attcattaaa
     3001 gtggcgaaag catgacagca atcacaaaaa aatgaaaata acaaaaagag aaaacacttt
     3061 tgccattttg ctaacaaaca ggaaggagat gcgagggaga acgcgctccc tcgagaggaa
     3121 atcagtgcag cgcggcagtc aaacccacgg ccacgatcaa accgaggacg ataatcgttg
     3181 ttaccagtga aaatttaagg tcggtgctca tcaagttttc tcctttttta ttaccacaca
     3241 aatagtgata ttacgcattt ttacacactg tgatgaaaaa atctcccgtc atttataatg
     3301 ataagtgttt ttaccacttc cccttttcgg caagatcggc caaaattcca cgcttacact
     3361 atttgcgtac tggccattga ccccttcctg acgctccgtg tcgtttttcc ggcgtaccgc
     3421 aacacttttg ttgtgcgtaa ggcgtgtaaa ggcaaacgtt taccttgcga ttttgcagga
     3481 gctgaagtta gggtctggag tgaaatggaa tggcaacaat aaaagatgta gcgaaacgag
     3541 caaacgtttc cactacaact gtgtcacacg taatcaacaa aacacgtttc gtcgctgaag
     3601 aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca ctactcccct agcgcggtgg
     3661 cgcgtagcct gaaggttaac cacaccaagt ctatcggttt gctggcgacc agcagcgaag
     3721 cggcctattt tgccgagatc attgaagcgg ttgaaaaaaa ttgcttccag aaaggttaca
     3781 ccctgattct gggtaatgcg tggaacaatc ttgagaaaca gcgggcttat ctgtcgatga
     3841 tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc tgagtaccca gagccgttgc
     3901 tggcgatgct ggaagagtat cgccatatcc caatggtggt gatggactgg ggtgaagcaa
     3961 aagctgactt caccgatgcg gtcattgata acgcgttcga aggcggctac atggccgggc
     4021 gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat ccccggcccg ctggaacgta
     4081 acaccggcgc aggccgcctt gccggtttta tgaaggcgat ggaagaagcg atgatcaagg
     4141 tgccggaaag ctggattgtt cagggtgact ttgaacccga atctggttat cgcgccatgc
     4201 agcaaatact gtcgcagccg catcgcccta ctgccgtctt ctgtggtggc gatatcatgg
     4261 caatgggcgc actttgtgct gctgatgaaa tgggtctgcg cgtcccgcag gatgtttcgc
     4321 tgatcggtta tgataacgtg cgcaacgccc gctattttac gccggcgctg accacgatcc
     4381 accagccaaa agattcgctg ggtgaaacag cgttcaacat gctgttggat cgtatcgtca
     4441 acaaacgtga agaaccgcag tccattgaag tgcatccgcg cttgattgaa cgccgctccg
     4501 tggctgacgg cccgttccgc gactatcgtc gttaatcacc cgttgcggga gtctcttccg
     4561 gctcccgcag ccactcctta ttcagcgtct cactatcgcc gagatactca agcagccagg
     4621 ttaacgcagg cgacatatca ttttgctgcc atgtcagaca acatgccgaa tccgggaagg
     4681 ggttttccaa ttcaagtgct acccacttcc cctcattaag ccacggtttg gcgaaatgtg
     4741 ttggcaccat ccctatgcat aatcctgccg agatacaggt tgccgatgat tcccagtcag
     4801 gcacgacaac tcttttttgg ttatccagta accaggtaat acgtttaggt agcgttcgcg
     4861 aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt atcatcgctg aatgggccat
     4921 ccatcaacgc cagcgggtgg tggctggcaa caacgcaact ccagcttagc atccccatat
     4981 cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt tgcgccaatc gccagttcca
     5041 cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac ttcctgaaag acaagaagtt
     5101 cgacatcatc aaaatggcga taaaaatcaa cgatcatctg ccgtgtacgt tctggcctga
     5161 caatattatc cactgcgata gctaactgac cgcgccagcc gttcgctatc tgctgacatt
     5221 gctggcgggt gatctgcatt tttttgacaa cagagcgccc ttctttgaga aaccacgctc
     5281 cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa gagcggcacc gccagccact
     5341 cttccagctg acgcacggta tagctgaccg cagaaggaac gcgatgcagc tcctgtgccg
     5401 cagcgctaaa actaccatta cgcgctaccg catcaacaac ttcgagtgaa tattctgacc
     5461 acatagtctg cctgcaaaat ttttgaaatc agtcatcaaa tattaccgtt tcacaacact
     5521 aatttcactc cctacacttt gcggcggtgt ttaattgaga gatttagaga atatacatgc
     5581 aacctgggaa aagattttta gtctggctgg cgggtttgag cgtactcggt tttctggcaa
     5641 ccgatatgta tctgcctgct ttcgccgcca tacaggccga cctgcaaacg cctgcgtctg
     5701 ctgtcagtgc cagccttagt ctgttccttg ccggttttgc cgcagcccag cttctgtggg
     5761 ggccactctc cgaccgttat ggtcgtaaac cggtgttatt aatcggcctg acaatttttg
     5821 cgttaggtag tctggggatg ctgtgggtag aaaacgccgc tacgctgctg gtattgcgtt
     5881 ttgtacaggc tgtgggtgtc tgcgccgcgg cggttatctg gcaagcgtta gtgacggatt
     5941 attatccttc acagaaagtt aaccgaattt ttgcgaccat catgccgctg gtgggtctat
     6001 ctccggccct ggctcctctg ttaggaagct ggctgctggt ccatttttcc tggcaggcga
     6061 ttttcgccac cctgtttgcc attaccgtgg tgctgattct gcctattttc tggctcaaac
     6121 ccacgacgaa ggccggtaac aatagtcagg atggtctgac ctttaccgac ctgctacgtt
     6181 ctaaaaccta tcgcggcaac gtgctgatat acgcggcctg ttcagccagt ttttttgcat
     6241 ggctgaccgg ttcaccgttc atccttagtg aaatgggcta cagcccggca gttattggtt
     6301 taagttatgt cccgcaaact atcgcgtttc tgattggtgg ttatggctgt cgcgccgcgc
     6361 tgcagaaatg gcaaggcaag cagttattac cgtggttgct ggtgctgttt gctgtcagcg
     6421 tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc gctggtcgaa atcctgatcc
     6481 cattctgtgt gatggcgatt gccaatggcg cgatctaccc tattgttgtc gcccaggcgc
     6541 tgcgtccctt cccacacgca actggtcgcg ccgcagcgtt gcagaacact ctacaactgg
     6601 gtctgtgctt cctcgcaagt ctggtagttt cctggcttat tagtatcagc acgccattgc
     6661 tcaccaccac cagcgtgatg ttatcaacag tagtgctggt cgcgctgggt tacatgatgc
     6721 aacgttgtga agaagctggc tgccagaatc atggcaatgc cgaagtcgct catagcgaat
     6781 cacactgatc tatatcgata tacttatact taggctgcta acaaaatttt gttgtatgat
     6841 tgaaattagc ggcctatact aatttcgagt tgttaaagct acgataaata ttatgttttt
     6901 acggggacag gatcgttccc gactcactat ggatggtcat ttcggcaagg gttcctcctt
     6961 tccctctgtt ctacgtcgga ttatagactc gcggtttttt ctgcgagatt tctcacaaag
     7021 cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg accagtgatg gagaaactat
     7081 gagttcatcg tgtatagaag aagtcagcgt accggatgac aactggtacc gtatcgccaa
     7141 cgaattactt agccgtgccg gtatagccat taacggttct gccccggcgg atattcgtgt
     7201 gaaaaacccc gattttttta aacgcgttct gcaagaaggc tctttggggt taggcgaaag
     7261 ttatatggat ggctggtggg aatgtgaccg actggatatg ttttttagca aagtcttacg
     7321 cgcaggtctc gagaaccaac tcccccatca tttcaaagac acgctgcgta ttgccggcgc
     7381 tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata gtcggcaaag agcattacga
     7441 tttgggtaat gacttgttca gccgcatgct tgatcccttc atgcaatatt cctgtgctta
     7501 ctggaaagat gccgataatc tggaatctgc ccagcaggcg aagctcaaaa tgatttgtga
     7561 aaaattgcag ttaaaaccag ggatgcgcgt actggatatt ggctgcggct ggggcggact
     7621 ggcacactac atggcatcta attatgacgt aagcatggtg ggcgtcacca tttctgccga
     7681 acagcaaaaa atggctcagg aacgctgtga aggcctggat gtcaccattt tgctgcaaga
     7741 ttatcgtgac ctgaacgacc agtttgatcg tattgtttct gtggggatgt tcgagcacgt
     7801 cggaccgaaa aattacgata cctattttgc ggtggtggat cgtaatttga aaccggaagg
     7861 catattcctg ctccatacta tcggttcgaa aaaaaccgat ctgaatgttg atccctggat
     7921 taataaatat atttttccga acggttgcct gccctctgta cgccagattg ctcagtccag
     7981 cgaaccccac tttgtgatgg aagactggca taacttcggt gctgattacg atactacgtt
     8041 gatggcgtgg tatgaacgat tcctcgccgc atggccagaa attgcggata actacagtga
     8101 acgctttaaa cggatgttta cctattatct gaatgcctgc gcaggtgctt tccgcgcccg
     8161 tgatattcag ctctggcagg tcgtgttctc acgcggtgtt gaaaacggcc ttcgagtcgc
     8221 tcgctaaagg ctattctatc gccccctctc cgggggcgat ttcagatcag gcttctgtac
     8281 ctggttgatt catggcattt tctcgtgccg ccagcacacg ttctaccgta tctaccactg
     8341 cctgagtttg tggatcaatt tcaatgttga cgcgtgcgcc gagttttttc ttcccaagag
     8401 tcgtgcgttc cagagtttcc ggaattaaat ggacgcaaaa acgcgttggc gtgacttcgc
     8461 cgacggtcag gctaataccg tcgatgccaa taaatccttt gtacaggata tatttcatca
     8521 actgactatc ctggacttta aaccagatct ggcgattatt ttccgaggtt aatattttcg
     8581 ccacttcagc ggtggtcata atatgacctg acattaagtg tccgccaatt tcatcactga
     8641 atttcgccgc acgctcaacg tttacccaat cccccacttt taaatcgcca agattggtaa
     8701 tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt gccgttaatt tccgtcacgg
     8761 tcaggcagca accgttatgc gccacggaag caccggtttc caggccgtcc agcatgtggt
     8821 cgggtaactc caccacatgc gtacgaaaat ttggtttctc gtcaatcgac accagttttg
     8881 cggtgccctg tacaatcccc gtaaacatac ttacaactcc tgaaatcagt taagacattc
     8941 tgttcagcac aatagcaggt ggcaaacgcc cttaccagtg aaggggtaag aatgactatt
     9001 ttttcactgg agaattaata aatcctcgct acaatagact gaatttcccc tgcttcttct
     9061 ttttgctgcc cattcaggcg gctttttagt ctctcatata actacaaata aaaggtgttc
     9121 acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc attagcaatc ccggtgattc
     9181 tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac cgtgatggcg ggcggctata
     9241 gtgccaccga catggcggcg gtcgctatcg gtacttctat ctggcttccg gcgatcctct
     9301 ttggtcacgg actgctgctg gcattaacgc cggttatcgc gcaattaaat ggttccggtc
     9361 gacgtgagcg cattgcgcat caggtgcgac aaggtttctg gctggcaggt tttgtttccg
     9421 ttctcattat gctggtgctg tggaatgcag gttacattat ccgctccatg gaaaacatcg
     9481 atccggctct ggcggacaaa gccgtgggtt atctgcgtgc gttgttgtgg ggcgcgccgg
     9541 gctatctgtt cttccaggtt gcccgtaacc aatgtgaagg tctggcaaaa accaagccgg
     9601 gtatggtaat gggctttatc ggcctgctgg tgaacattcc ggtgaactat atctttattt
     9661 atggtcattt cggtatgcct gagctcggtg gcgttggttg tggcgtggct actgcggcgg
     9721 tgtattgggt catgttcctt gccatggttt cttacattaa acgcgcccgc tccatgcgcg
     9781 atattcgtaa cgaaaaaggc accgcgaaac ccgatcctgc ggttatgaaa cgactgattc
     9841 aactcggttt accgattgcg ctggcactat tctttgaagt gacactgttt gccgtcgtgg
     9901 ctctgttagt gtctccgctc ggtattgttg atgtcgcagg acaccagatt gccctgaact
     9961 ttagttcact gatgtttgtg cttccaatgt cgctggcggc agcggtaact atccgcgtag
    10021 gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac cgctgcgcgg accgggctta
    10081 tggtgggtgt ctgtatggca accctgacgg ccattttcac ggtttcactc cgggagcaaa
    10141 tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct ggctgcgcat ttgatgttgc
    10201 tggcggcggt atatcagatt tctgactcaa tccaggtgat tggcagtggg attttgcgtg
    10261 gttataaaga tacgcgttcc attttctata tcacctttac agcttactgg gtgctgggct
    10321 tgccaagcgg ctatattctg gcactgaccg atctggtcgt tgaacctatg gggccagcag
    10381 gcttctggat aggctttatt atcggcctga catcggcagc cattatgatg atgttgcgta
    10441 tgcggttcct gcaacgtctg ccgtcagtta tcattctgca acgagcatcc cgctaataaa
    10501 gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac gcaggctgga gcgttgcgcc
    10561 aatcccgtct tcgtctggct gtaatttcag agcgttgcac agagtttgcc gactggcatc
    10621 tgttatctca acgtgtatat caccggtaac ttcccctttc acattgatga agtcaggtga
    10681 gatttctttc gcactggttt gcaggcaaat cgctccctct ccgcgcaggc gtttcacttt
    10741 cagttgcaca tgcgttgccg caccagtgat cagccacagt tttccgccat tcagattgat
    10801 gttaccaaca ataaaagtga caccgtcacc ttttaccgta ctgccgtcga gaatcaacac
    10861 accgccctgc tgtacagtaa tcgttgattg ttgcagatct gcgccatagc caataatcat
    10921 tgaagcgccg tccagtaatg caacactccc ggtcagttca accggtgcgc gatcttcctc
    10981 tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg atcactgttc caccttgtat
    11041 taccgcattt tccagcgtac cggcatgcac ttcagcccga ccacccacat tcacggacag
    11101 gttttgcgtc ttaccggaac tgtagagctg aaggccatcc gtccccagac gataaatgga
    11161 tccattctca acaatggtgt cagagtcttc accaccgaga ttattcatgg tgccaccagc
    11221 aagcaacgta tcactggctt tcccggccag cataaaaacg ccgccctgat taatctcaca
    11281 attgctggct tccccacctg gatacaccaa caattcacca ccttcatcta ccacgaccgc
    11341 tgaggttgtc ccattaacca acaggccgcc ctcttgatcg agaatgattt tttccgcgcg
    11401 atgtccttcc agtacacgca ggttaccgcc attttccagc aacaaaccgc aggcataacc
    11461 ctgatcaacg ctgaattcac cttcgggatg gcggccatta accgtagcga gcgtagagag
    11521 actaattgcg cctccctcgg actgaacaat atctgtggca atggcaccgt cgctgaccga
    11581 tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc ccaccggttg ccactttcaa
    11641 ccagccagcc cccgtgacta cggtattttc ggctcttccg ccagcttcaa caatcaactc
    11701 gccttcagta agttgcgtcg cgccaatgac ctcgccggtc ttaacaattt gcacattccc
    11761 gtcgctcatc aagtttttcg catcagatcc catgatttat tcctttgctg catctgtgtg
    11821 cctttattgc tacctaaatg taaaggctac ggaggattta tccacgacag atttgagatg
    11881 gtggcaaaca actctgttta aactctgata cacgaattat tgggttgtat cagatgtaaa
    11941 tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa tatagccaat ttgcttaaaa
    12001 gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt gcctcttgcc accttcccac
    12061 tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt gcgttcatag ctcagttggt
    12121 tagagcacca ccttgacatg gtgggggtcg ttggttcgag tccaattgaa cgcaccatcc
    12181 tgcgtccgta gctcagttgg ttagagcacc accttgacat ggtgggggtc ggtggttcga
    12241 gtccactcgg acgcaccaga ttttcttaat ctggtcttct cctttttccc tctgtttctt
    12301 ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt atgcgttatc agaaggagaa
    12361 tcgctatggc aactttgtta caacttcatt ttgcttttaa tggcccattt ggcgacgcaa
    12421 tggctgagca gcttaaaccg cttgctgagt cgattaatca ggaacctggt tttctgtgga
    12481 aggtatggac agaaagtgaa aagaaccacg aagccggtgg gatctatctt tttactgatg
    12541 aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg acttaaaaat ctcggcgttg
    12601 aggaagttgt cgccaaagtt ttcgatgtca atgagccact gagtcaaatc aatcaggcaa
    12661 aactcgcctg acagaattta atcaagggcg gttagcgccc ttttcatccc tgtctgaaat
    12721 ttctcaaatt ctaaaaatct caaccaaact tatctgataa cactaaattc gaaagaatgc
    12781 gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt gccgggccta cggggatcta
    12841 caccttattc tcgcttctac agcaacaaac tccactttct atttcta
//
LOCUS       DAJTSL010000115        12868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTSL010000115 DAJTSL010000000
VERSION     DAJTSL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.115"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            47..520
                     /locus_tag="OO433_002925"
                     /pseudo
     CDS             47..520
                     /locus_tag="OO433_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
     gene            complement(529..927)
                     /gene="vapC"
                     /locus_tag="OO433_002926"
     CDS             complement(529..927)
                     /gene="vapC"
                     /locus_tag="OO433_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6051328.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(927..1154)
                     /gene="vapB"
                     /locus_tag="OO433_002927"
     CDS             complement(927..1154)
                     /gene="vapB"
                     /locus_tag="OO433_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6051329.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            1236..1655
                     /locus_tag="OO433_002928"
     CDS             1236..1655
                     /locus_tag="OO433_002928"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6051330.1"
                     /translation="MMSIAQVRSAGSAGNYYTNKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTDDDGRTVRNGADR"
     gene            1642..6513
                     /gene="traI"
                     /locus_tag="OO433_002929"
     CDS             1642..6513
                     /gene="traI"
                     /locus_tag="OO433_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCR6051331.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSYAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRYVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAAARMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQA
                     IAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQV
                     PRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYAAIVRDYTGRTP
                     EAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVPVLNTANIRDGELRRLST
                     WENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTI
                     RVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHID
                     LAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQYVQVYTDNRQGW
                     TDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDS
                     PARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDA
                     QFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGD
                     IPDNSVQPGAGNGEPVMAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE
                     QAVREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDM
                     VRDLQKEKTPGGD"
     gene            6533..7279
                     /gene="traX"
                     /locus_tag="OO433_002930"
     CDS             6533..7279
                     /gene="traX"
                     /locus_tag="OO433_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6051332.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7334..7894
                     /locus_tag="OO433_002931"
     CDS             7334..7894
                     /locus_tag="OO433_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6051333.1"
                     /translation="MTEQKRPVLTLKRKTEGTATVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            8025..8237
                     /locus_tag="OO433_002932"
     CDS             8025..8237
                     /locus_tag="OO433_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6051334.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            8481..8942
                     /locus_tag="OO433_002933"
     CDS             8481..8942
                     /locus_tag="OO433_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6051335.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8988..9197
                     /gene="hha"
                     /locus_tag="OO433_002934"
     CDS             8988..9197
                     /gene="hha"
                     /locus_tag="OO433_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6051336.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            9236..9826
                     /locus_tag="OO433_002935"
     CDS             9236..9826
                     /locus_tag="OO433_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6051337.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQTTLLTEREVSFMKGLFRIVDMTRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            10066..10326
                     /gene="repA"
                     /locus_tag="OO433_002936"
     CDS             10066..10326
                     /gene="repA"
                     /locus_tag="OO433_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6051338.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            10550..10624
                     /gene="tap"
                     /locus_tag="OO433_002937"
     CDS             10550..10624
                     /gene="tap"
                     /locus_tag="OO433_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6051339.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10617..11474
                     /gene="repA"
                     /locus_tag="OO433_002938"
     CDS             10617..11474
                     /gene="repA"
                     /locus_tag="OO433_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6051340.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(12554..>12868)
                     /locus_tag="OO433_002939"
     CDS             complement(12554..>12868)
                     /locus_tag="OO433_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6051341.1"
                     /translation="GIRRGQRYRLVFQFPIREQAAAFLGVVPVEKYSGTSVRGCPKLS
                     KIGPPEIRAKLYLASLCGLRFNPLMKAMYERLCLRGKAKMCAIGALMRKLVHWCYGVL
                     KT"
BASE COUNT         3127 a         3251 c         3803 g         2687 t
ORIGIN      
        1 ttaaaatcac catcatacac taaatcagta agttggcagc attaccaaat atcaggaacg
       61 accgaaggtc gctccggagt ttattccgcg tgacatcaac ccggaaatgg agaaccgtct
      121 gagtgccgta cttgccgcaa gggaagcaga aggtcgtcag atggccagcc tcttcgaacc
      181 ggatgtcccg gaggttgttt ccggagaaga cgtgactcag gctgaacagc cgcaacagcc
      241 ggtgtctcct gtcatcaacg ataagaagtc agattcaggt gtgaatgttc cggcaggggg
      301 gatcgagcag gagctgaaaa tgaaaccgga agaagagatg gaacagcaac tgccacccgg
      361 gatcagtgaa tccggtgaag tggtggatat ggccgcttat gaggcatggc agcaggaaaa
      421 tcatccggac acctggcagc agatgcagcg tcgtgaagag gtgaacatca atgtgcaccg
      481 ggaacgcggg gaggatgttg agccgggaga tgatttctga tgaacaggtc agctccagtc
      541 ttcagttctc aggccgccca cacgttcaaa ttcccgggtg ttattagtca caataatcag
      601 cccccgactg cgggcatgac ctgcgatcat ttgatcaaat ggcccgacag ggcgtccctg
      661 acgggcaagt tctgctctta tctggccggt gtgtgtggct gcagcagcgt cgtaatccag
      721 aacatcaatg cgggaaacaa atccctcgat cacagcgaga ttacgttcag gcatctggct
      781 tttttctgca ccatatatca gctccatcag ggtgaccgaa ctgatacaca ttctcccctg
      841 gttcaggtta aaacgctccc tgacgctggc gggtttgttc tttatcgtaa aaatgcagat
      901 gttggtatcg agcataaact tcagcatcag aatgactccc tttcctgcat gccgggctgt
      961 tccctgttat ccataaaatc ggcgctgacg ctgtgtccgt cgaaccattc gtcccatgtc
     1021 tctccggctg gcgtaatgat tctggttcgt ccgacagcaa tcacttcaac gcgttttacg
     1081 ttttccggca atgcaaccgc ttttggcagt ctgaccgctt ggctgcggtt gctgagaaat
     1141 acggtggttt ccattatttg cctccttatg tggatatgtc ttgtgtatat ccattgtggg
     1201 attgataccg ggatatgtca aagggatata cgtttatgat gagtatcgcg caggtcagat
     1261 cggccggaag tgccggtaat tattatacca acaaggataa ttactatgtg ctgggcagca
     1321 tgggagaacg ctgggccggc cagggggctg aacaactggg gctgcagggc agtgtcgata
     1381 aggatgtctt tacccgcctt ctggagggca ggctgccgga cggggcggat ttaagccgca
     1441 tgcaggatgg cagtaacagg catcgtcccg gctacgacct gaccttctcc gctcccaaaa
     1501 gtgtctccat gatggccatg ctcggggggg acaaacgtct tattgaagca cataaccagg
     1561 ccgtggattt tgctgtccgt catcacccgt gtggaggcac tggcctccac acggatgatg
     1621 acggacggac agtcagaaac ggtgctgacc ggtaatctgg tgatggcact gtttaaccac
     1681 gacaccagtc gcgatcagga accacagtta cacacgcatg cggtggtgac taatgttacg
     1741 cagcataacg gcgaatggaa gacactgagc agtgacaaag tggggaaaac ggggttcatt
     1801 gagaatgtgt acgctaatca gattgccttt ggcaggctct accgggaaaa actgaaagag
     1861 caggttgagg cgctgggcta tgaaactgaa gtggtcggta agcacggtat gtgggaaatg
     1921 ccgggtgtac cggtggaggc cttttccgga cgctcacaga ctatccggga ggccgtcggg
     1981 gaagacgcct cgctgaaatc ccgggatgtg gcggcgctgg atacgcgtaa atccaaacag
     2041 cacgtcgatc ctgaagtcag aatggccgag tggatgcaga cgctgaagga aaccgggttc
     2101 gacatccggg catatcgtga cgcagcggaa caacgggcat atacccgcac gcagacaccc
     2161 ggacccgctt cacaggacgg gccggatgtg cagcaggcgg tgacacaggc cattgccgga
     2221 ttaagtgaac gcaaagtgca gtttatgtat acggacctgc tggccaggac ggtcggcata
     2281 ctgccgccgg aaaatggtgt gattgaacgg gcacgcgccg gtatcgatga ggccatcagc
     2341 cgtgagcagc ttatccccct cgatcgtgag aaggggctgt tcacgtccgg gattcatatg
     2401 ctcgatgagc tgtcagtccg ggcactcagt cgtgacatca tgaaacagaa ccgggtgacc
     2461 gtacatccgg agaaaagtgt cccccggacg gccggttaca gctatgccgt cagtgttctg
     2521 gcacaggacc gtccgtcgct ggccattgtg tccgggcagg gcggtgcagc cgggcagcgt
     2581 gagcgggtgg ctgaactggt catgatggcc cgggagcagg ggcgggaggt gcagattatc
     2641 gctgctgacc gtcgctcgca gatgaacctg aagcaggatg aacggctgtc cggtgaactg
     2701 ataaccggac gtcgtcagtt gctggaaggc atggccttca cgccgggcag taccgttatc
     2761 gtcgaccagg gcgaaaaact ctccctgaaa gagacgttaa ccctgctgga tggtgccgca
     2821 cgtcataacg tacaggtcct gataactgac agcgggcagc gaaccggtac aggcagtgca
     2881 ctgatggcca tgaaggatgc cggggtgaac acatatcgct ggcagggggg agaacagcga
     2941 ccggccacca tcatcagtga accggaccgt tatgtccgct atgcccggct ggccggagat
     3001 tttgcggcca gcgtgaaagc cggagaagag agcgtggcac aggtcagcgg agtacgggaa
     3061 caggccatac tgacacaggc cattcgcagt gagctgaaaa cacagggcgt gctcggacac
     3121 ccggaggtaa ccatgaccgc cctgtcaccg gtctggctgg acagccggag ccgttatctg
     3181 cgggatatgt accgcccggg aatggtgatg gagcagtgga acccggagac acgcagtcat
     3241 gaccgctatg tgacagaacg tgtgacggcg cagagtcaca gcctgaccct gcggaatgcg
     3301 cagggcgaaa cgcaggtggt gcgtatttct tccctggaca gcagctggtc gctgttccgg
     3361 ccggaaaaaa tgccggtggc agacggtgag cgtctgaggg tgacagggaa aattcccgga
     3421 ctccgcgtct ccggcggtga ccgcctgcag gtggcatccg tcagtgaaga tgcgatgacg
     3481 gttgttgtgc cgggacgggc tgaaccggcc accctgcctg tgagcgattc accgttcacg
     3541 gcactgaagc tggagaacgg ctgggtggaa acgcccgggc attccgtcag cgacagtgcg
     3601 acggtttttg cctccgtcac acagatggca atggacaatg ccaccctgaa cggtctggcc
     3661 cgcagtggtc gtgatgtccg gctgtattcc tcactggatg aaacccgtac tgcggaaaaa
     3721 cttgcccgcc atccttcctt tacggtggtt tctgagcaga taaaggcgcg tgccggtgag
     3781 acattgctgg aaaccgctat cagtctgcag aaagccgggc ttcacacgcc ggcacagcag
     3841 gccattcatc tggcccttcc tgtgctggaa agtaaaaacc tggccttcag catggtggac
     3901 ctgctgacag aggcgaagtc gtttgctgca gaaggaaccg gttttgctga cctgggaggg
     3961 gaaatcaatg cgcagataaa acggggtgat ttactgtatg tggatgtggc aaaaggctat
     4021 ggcacaggcc tgctggtttc ccgtgcgtcg tatgaggcag aaaagagcat tcttcgccat
     4081 attctcgaag gtaaggaggc ggtcacgccg ctgatggaga gagtacctgg cgaactcatg
     4141 gagaaactga catcaggaca gcgcgccgcc gcccgtatga tactggaaac gtccgaccgt
     4201 ttcacggtgg tgcagggcta tgccggtgtg ggtaagacca cacagttccg ggcggtgatg
     4261 tcagccgtga acatgctgcc ggagagtgaa cgtccccgtg tcgtggggct ggggcccaca
     4321 caccgtgcgg tcggggagat gcgcagcgcc ggcgtggatg cacagacact ggcgtccttt
     4381 ctgcatgaca cgcagctgca gcagcgcagc ggagaaacgc cggatttcag caacacgctg
     4441 ttccttctcg atgagagctc tatggtgggc aataccgaca tggcacgggc atacgccctg
     4501 attgcggccg gtggcggtcg ggctgtggcc agcggtgaca cggaccagct gcaggccatc
     4561 gcgcccggtc agcctttccg tctccagcag acgcgcagtg ctgccgatgt ggtcatcatg
     4621 aaggagattg tgcgtcagac gccggaactg cgggaggcgg tatacagcct gattaaccgg
     4681 gatgtggaaa gggcgctgtc cgggcttgag cgtgtgaaac cgtctcaggt gccacgtctg
     4741 gagggcgcat gggcaccgga gcactccgtg acggagttca gtcacagcca ggaagcgaaa
     4801 ctggcagaag cgcagcagaa ggcgatgctg aaaggcgagg cttttccgga tgtccccatg
     4861 acactgtatg cagccattgt ccgcgactat accggcagga caccggaagc acgggagcag
     4921 acgctgattg tcacgcacct gaatgaggac cggcgcgtac tgaacagcat gattcatgat
     4981 gcacgggaaa aggccggaga actggggcag gtgcaggtca tggtgcctgt cctgaacaca
     5041 gcgaatatac gcgacgggga gctgcgtcgt ctctccacct gggagaataa cccggatgcc
     5101 cttgccctgg tggataatgt gtatcaccgg attgccggta tcagcaagga tgacgggctg
     5161 ataaccctgc aggatgcgga aggtaacacg cggctgattt cgccccggga ggcggtggct
     5221 gaaggcgtca cgctgtacac cccggacacc atccgggtgg ggaccggtga caggatacgc
     5281 ttcacgaaga gtgaccggga gcgcggttat gtggccaaca gcgtctggac ggtgacagcg
     5341 gtttccggtg acagtgtcac actttctgac ggtcagcaga cccgtgtgat tcgccccggt
     5401 caggagcggg cagagcaaca tattgacctg gcctatgcca tcaccgccca cggtgcacag
     5461 ggggcgagtg aaacctttgc catcgcgctt gaaggcacgg aaggtaaccg gaaactgatg
     5521 gccggctttg agtcagccta cgtggccctg tcgcgtatga agcagtatgt gcaggtgtac
     5581 accgataacc gtcagggctg gacggatgcc atcaacaatg ccgtacagaa aggaactgcc
     5641 cacgatgtgt ttgagccgaa accggaccgg gaggtcatga atgcagagcg gctgttcagt
     5701 acggcgcggg agctgcggga cgtggcggca ggccgtgccg ttctccgtca ggcggggctg
     5761 gccgggggag acagtcctgc acggtttatt gctccgggac gtaaatatcc gcaaccgtat
     5821 gtggcactgc cggcgtttga ccgtaacggc aagtccgccg gtatctggct gaacccgctg
     5881 accacggatg acggaaacgg gctgcgggga ttcagcggtg aaggtcgggt aaaaggcagc
     5941 ggggatgcgc agttcgtggc cctgcagggc agccgtaacg gagaaagcct gctggctgat
     6001 aatatgcagg atggcgtccg gattgcccgt gataatcctg acagtggtgt ggtggtaaga
     6061 atcgccggtg aaggccgtcc gtggaatccc ggtgccatca ccggtggccg cgtgtggggg
     6121 gatatcccgg acaacagcgt ccagccggga gccggaaatg gcgaaccggt catggcagag
     6181 gtactggcac agcggcaggc tgaagaggcc atccgccgtg aaacggaacg tcgcgcagat
     6241 gaaattgtcc gtaaaatggc agagaacaaa cctgacctgc cggacggcaa aacagagcag
     6301 gctgtcaggg aaattgccgg gcaggagcgt gaccgggctg ccataactga acgggaagcc
     6361 gcgctgccgg agagtgtact gcgtgaacca caacgggtgc gggaagcggt ccgggaagtt
     6421 gcccgggaaa atttgctgca ggagcgtctg cagcagatgg agcgggatat ggttcgtgac
     6481 ctgcagaaag agaaaacccc gggcggagac tgatacagga agacaaacac tgatgacaac
     6541 ggataacacg aacacgacac gtaacgattc actggctgca cggaccgata cctggttgca
     6601 gtcatttctg gtctggtcac ccggacagcg ggatatcatc aaaacggtgg cactggtgct
     6661 gatggtactg gaccacatca atctgatatt ccagctgaag caggaatgga tgtttctggc
     6721 cgggcgagga gcctttccgc tttttgccct ggtgtggggg ctgaatctgt cccgtcatgc
     6781 gcatatccgg caaccggcca ttaaccggct gtggggatgg ggaattattg cccagttcgc
     6841 atattatctg gcaggctttc cctggtatga ggggaatatc ctgtttgcct ttgcagtggc
     6901 ggcacaggtg ctgacgtggt gtgaaacgcg cagtgggtgg cgtacagcag cggccatact
     6961 cctgatggcc ctgtgggggc ctttgtccgg caccagttac ggtattgccg ggttgctgat
     7021 gctggcagtc agcaaccggc tgtaccgggc ggaagacaga gcggaacgtc tggcgctggt
     7081 cgcctgcctg ctggctgtta ttccggcgct taaccttgcc accagtgatg cagcggcggt
     7141 ggccggtctt gtgatgacgg tgctgaccgt tggtctggtg ttgtgtgccg gaaaatcatt
     7201 accccgtttc tggcccggag actttttccc gacgttctat gcctgtcatc tggctgtgct
     7261 gggcgttctg gcgctgtgac gggtgtggta tctttggccg caagaggatg attcgtcaga
     7321 ggcagaacac agcatgacag agcagaagcg accggtactg acactgaagc ggaagacaga
     7381 gggaacagcg actgtccgca gccggaaaac catcatcaat gtcaccacgc caccaaagtg
     7441 gaaggtgaaa aagcagaagc tggccgagaa agcggcccgt gaagcagagc tggcggcaaa
     7501 aaaagcgcag gccagacagg cgctgtccat ttatctgaac ctgcccacgc tggatgaggc
     7561 tgtgaatacc ctgaagccct ggtggccggg attatttgac ggtgacacgc cccggcttct
     7621 ggcctgcggt atccgggacg tgttactgga agacgtggcg cagcggaata tcccgctctc
     7681 gcataaaaaa ctgcgcaggg cgctgaaggc catcacccgt tcagaaagct atctgtgtgc
     7741 catgaaagcc ggtgcctgcc ggtatgacac ggaagggtat gtgacggagc atatttctca
     7801 ggaggaggaa gcgtatgcgg gagcgcgtct ggcgaaaatt cgtcgccaga accggataaa
     7861 ggcagaactt caggccgtgc ttgatgagaa ataaaaaaag cctcccccag agagaggagg
     7921 cagggaaata aggcttaaaa ggaaataact tccgcttccg aaaggaagcc agtccactga
     7981 attatttatc acaataatac tgacaactaa agggggacaa gaaaatgaat ggattcagaa
     8041 acagttcacg gaacggccag gtctggcgtt accagcgtgc cggcgggcgg gccgttattc
     8101 tggaagtcag tggacgctgg atggaagcgg cagaagcatg gcgacgggct gcctgtgttg
     8161 cgcctaggac agactggcag cagtttgccc ggaaaagggc tgaacactgc caccggcgct
     8221 gccggggaag ggggtaacga aaaaagcagc cttcattact ccaggagtca gaaggctgaa
     8281 aggagtttat gagatttgct tcttaatatc cggtcgggca gtatgaccgg acagttctga
     8341 gtagcacaga gtcttttccg cattaagtcg attttttata ttttgtgatt aatttcacaa
     8401 aataaggtgt tattcaatgt gtgctgcact attcaggggg gctgaaatac aggtgccatt
     8461 tatctgatat ggagaataac atgaggaaat atattccact ggtattattt atcttttcat
     8521 ggccggtatt gtctgcagat attcatggac gggttgttcg tgtactggac ggtgatacca
     8581 tcgaggtcat ggactcactg aaggcggtgc ggatcaggct ggtaaatatt gatgcaccgg
     8641 aaaagaaaca ggattatggc cggtggtcaa cagacatgat gaaatcactg gttgcaggaa
     8701 aaacggttac cgtcacatat tttcagcgag atcgttatgg tcgtattctc gggcaggttt
     8761 atgcaccaga cggaatgaat attaatcagt ttatggtccg ggccggggct gcctgggtat
     8821 atgagcagta caacacagat cccgtgctac ctgtccttca gaacgaagcc agacaacaaa
     8881 aacgtgggct ctggtcagat gctgatccgg ttcccccctg gatatggagg caccgtaaat
     8941 aaagcgatgg caatcctatc attcatctgt tctcagcggg aataaaaatg gcaaaaacaa
     9001 aacaggaatg gctgtatcag ttacggcgat gttcatcagt caatacgctg gaaaaaatta
     9061 ttcataaaaa cagagacagt ttatcaaaca gtgaacgtga atcatttaat tcagcagcag
     9121 accaccggct agcggaatta atcaccggaa agttatatga caggatacca aaagagatat
     9181 ggaaatatgt aaggtaataa ttcagcaata attaattgcc aggagcagat aaataatgaa
     9241 gttgattatt tttattctca ttgttctgat tattgcagcg ctacttatcc ggattatcct
     9301 cagaagtgta aatcagcatt ctcctttgct gatgcaattg catgcggcag gtatacggac
     9361 cggtgatgca gagcgaatac tgtccagcgg tgaatactgg caacgtcaga cgacgctgct
     9421 gacagaaagg gaagtcagtt ttatgaaagg actgttcaga attgtggata tgacgcggtg
     9481 gtatctgtgt ccgcaggtac gggtcgcgga tatcgtccag ctgaacggga atatccggcc
     9541 acgatcacgc cagtggtggc agttattcag gatggtgtct cagtggcatg ttgatgtggt
     9601 tatcgttgag ctgcgttcgt tcagtattgt tgcagcagta gagctggatg atgccagcca
     9661 tttacgaccg gaacgcagac gccgggatat tcttctggaa gaggttctga ggcaggctgg
     9721 tattccgttg ctcagaagcc acgatgccag aaaactgctg cagatgaccg gagaatggct
     9781 gaatacaaca ggggctgatc agcagtcacc ggaacatcgt agctgacgcc ttcgcgttgc
     9841 tcagttgtcc aaccccggaa acgggaaaaa gcaagttttc ctcgctcccg gcgtttccat
     9901 aactgaaaac catactattt cacagtttaa atcacattaa acgacagtaa tccccgttga
     9961 tttgtgcgcc aacacagatc ttcgccacaa ttctcaagtc gctgatttca aaaaactgta
    10021 gtatcctctg cgaaacgatc cctgtttgag tattgaggag gcgagatgtc gcagacagaa
    10081 aatgcagtga cttcctcatt gagtcaaaag cggtttgtgc gcagaggtaa gcctatgact
    10141 gactctgaga aacaaatggc cgctgttgca agaaaacgtc ttacacacaa agagataaaa
    10201 gtttttgtca aaaatcctct gaaagatctc atggttgagt actgcgagag agaggggata
    10261 acacaggctc agttcgttga gaaaatcatc aaagatgaac tgcagagact ggatatacta
    10321 aagtaaagac tttactttgt ggcgtagcat gctagattac tgatcgttta aggaatttta
    10381 tggctggcca cgccataagg tggcagggaa ctggttctga tgtagatgta caggagccag
    10441 aaaagcaaaa accccgataa tcttctttaa ctttggcgag tcagaaagat taccggggcc
    10501 cacttaaacc gtatagccaa caatcaagct atgcggggag tatagttata tgcccggaaa
    10561 agttcaagac ttctttctgt gctcgctcct tctgcgcatt gtaagtgcag gatggtgtga
    10621 ctgatcttca acaaacttat taccgccagg taaagaaccc gaatccggtg ttcacacccc
    10681 gtgaaggtgc ccggacgctt ccgttctgcg ggaaactgat ggaaaaggcg gtgggcttca
    10741 cctcccgttt tgatttcgcc attcatgtgg cacatgcccg ttcgcttggt ctgcgtcggc
    10801 gcatgccacc ggtactacgt cgccgggcca ttgatgcgct gctgcagggg ctgtgttttc
    10861 actatgaccc actggccaac cgcgtccagt gttccatcac cacgctggcc attgagtgcg
    10921 gactggcgac ggagtctgct gccggaaaac tctccatcac ccgggccacc cgggccctga
    10981 cgttccttgc agagctggga ctgattacct accagacgga atatgacccg cttatcgggt
    11041 gctacattcc gaccgatatc acgttcacat ctgcactgtt tgctgccctc gatgtatcag
    11101 aggaggcagt ggccgccgcg cgccgcagtc gtgtggaatg ggaaaacaga cagcgcaaaa
    11161 agcaggggct ggataccctg ggtatggatg aactgatggc gaaagcctgg cgttttgttc
    11221 gtgagcgttt ccgcagttac cagacagagc ttaagtcccg tggaatgaag cgtgcccgtg
    11281 cgcgtcgtga tgcggacagg cagcgtcagg atattgtcac cctggtgaaa cggcaactga
    11341 cgcgtgaaat ctcggaaggg cgcttcactg ccagtcgtga ggcggtaaaa cgtgaagtgg
    11401 agcgtcgtgt gaaggagcgc atgattctgt cacgtaaccg taattacagc cggctggcca
    11461 cagcttcccc ctgaaagtga cctcctcaga ataatccggc acgcgccgga ggcattcgca
    11521 cgcctgaagc ccgccagtgc acaaaaaatc agcgctacat acaaaaaaca acctcatcat
    11581 ccaccttctg gtgcatccgg tcccccctgt ttttgataca aaacacgcct cacagacggg
    11641 gaattttgct tatccacatt aaactgcaag ggacttcacc ataaagttac aaccgttcac
    11701 gtcataaagc gccagccgtc agccctacag ggtgcaatgt atcttttaaa cacctgttta
    11761 tatctccttt aaactactta attacattca tttaaaaaga aaacctgttc actgcctgtc
    11821 ctgtggacag acagatatgc acctcccacc acaagcggcg ggccccgacc gaagccggtt
    11881 tagttacaac atgcaaacat aaacccctaa aaaacccaag tccgacgcag aactgaaacc
    11941 acaaagcccc tccctcataa ctgaaaagcg gccccgcccc ggcccgaagg gccggaacag
    12001 agtcgctttt aattatgaat gttgtaacta tattatcatc gctgtcagtc ctctggctgg
    12061 acgtatcgag tacacgctcg taagcggccc tcacggccct cacggccctc acggccctca
    12121 cggccctcac ggcccgctaa cgcggagata cgccccgact gcgggtaaac ccttgtcggg
    12181 ctccgaccgc gcacagaagc tctgtcatgg ctgaaaacgg gtatggcttg gcagctacca
    12241 tgttctgtca agaaaaatcg cttgaactcc gacgtacagc cccctaagta ctgccagcaa
    12301 gctgtcagca ctaagtcccc gatacagcgc ggcgtagccg cgctaacggg gttaaaaggg
    12361 cttacctata cttcatcatc gctgaaagcc cgcatggcct cgcagggcag agatgggata
    12421 ggtgaaacct atcaatacgt accggcttac gccgggcttc ggcggtgtta ctcctgtacc
    12481 atatgtaact acatcatgcc gccttacatg cgctacgcgc atatgaagac aaatagccca
    12541 catcaaacgg tttttaggtc ttcagtacgc catagcacca gtgcacgagt tttctcatca
    12601 gcgcaccaat ggcacacatt ttggccttac ctctcaggca taatcgctca tacatcgctt
    12661 tcatcaatgg gttaaatctc agaccacaca acgaagccag atacagtttc gcccggattt
    12721 ccggcggacc aatttttgac agtttcggac accctctgac cgaggtgccg gaatattttt
    12781 caacgggaac gacacctaaa aatgcagccg cctgttcacg tataggaaat tgaaaaacca
    12841 gacgataccg ctgtccgcgc cttatgcc
//
LOCUS       DAJTSL010000116        12844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTSL010000116 DAJTSL010000000
VERSION     DAJTSL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.116"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            51..995
                     /gene="wecH"
                     /locus_tag="OO433_002940"
     CDS             51..995
                     /gene="wecH"
                     /locus_tag="OO433_002940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6051342.1"
                     /translation="MVVMIHTTTWYVTNAHSVSPVTWDIANVLNSASRVSVPLFFMIS
                     GYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINMELALKNLLQKPVFYHLW
                     FFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTVPQKIDGFEWLPINLYIN
                     GDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFIISRGTLYELQWRGNFADT
                     WYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLGIYGFHALIIHALRTRGI
                     ELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(1037..1474)
                     /locus_tag="OO433_002941"
     CDS             complement(1037..1474)
                     /locus_tag="OO433_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6051343.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(1520..1861)
                     /locus_tag="OO433_002942"
     CDS             complement(1520..1861)
                     /locus_tag="OO433_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051344.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(2026..2217)
                     /locus_tag="OO433_002943"
     CDS             complement(2026..2217)
                     /locus_tag="OO433_002943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051345.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(2150..3478)
                     /gene="xylB"
                     /locus_tag="OO433_002944"
     CDS             complement(2150..3478)
                     /gene="xylB"
                     /locus_tag="OO433_002944"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6051346.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGCGASTGRSKAGADCGESREIAH"
     gene            complement(3595..4872)
                     /gene="xylA"
                     /locus_tag="OO433_002945"
     CDS             complement(3595..4872)
                     /gene="xylA"
                     /locus_tag="OO433_002945"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCR6051347.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVSFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGR"
     gene            5238..6209
                     /gene="xylF"
                     /locus_tag="OO433_002946"
     CDS             5238..6209
                     /gene="xylF"
                     /locus_tag="OO433_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6051348.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGG
                     AIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGN
                     GQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6287..7828
                     /gene="xylG"
                     /locus_tag="OO433_002947"
     CDS             6287..7828
                     /gene="xylG"
                     /locus_tag="OO433_002947"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6051349.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            7806..8986
                     /gene="xylH"
                     /locus_tag="OO433_002948"
                     /pseudo
     CDS             7806..8986
                     /gene="xylH"
                     /locus_tag="OO433_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
     gene            9064..10242
                     /gene="xylR"
                     /locus_tag="OO433_002949"
     CDS             9064..10242
                     /gene="xylR"
                     /locus_tag="OO433_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCR6051350.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNREVML
                     "
     gene            complement(10350..11174)
                     /locus_tag="OO433_002950"
     CDS             complement(10350..11174)
                     /locus_tag="OO433_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6051351.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            11494..>12844
                     /locus_tag="OO433_002951"
     CDS             11494..>12844
                     /locus_tag="OO433_002951"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6051352.1"
                     /translation="MKLASCFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     "
BASE COUNT         3341 a         3171 c         3048 g         3284 t
ORIGIN      
        1 tgcagcccaa aatttactgg attgataacc tgcgagggat agcgtgttta atggtggtga
       61 tgattcacac cactacctgg tatgtgacca atgctcatag tgttagcccc gtcacatggg
      121 atatcgccaa tgttctgaat tctgcctctc gtgtcagcgt gccgctattt ttcatgattt
      181 ccggctatct cttttttggc gaacgcagcg cccagccgcg ccatttcttg cgtatcggct
      241 tatgtctgat tttttatagc gcaatcgcac tgctctacat tgcgctgttt acctccatca
      301 atatggagtt agcgctgaaa aacctgctgc aaaagccagt gttttaccac ttgtggtttt
      361 tcttcgcgat tgcggtgatt tatctggttt caccgctgat tcaggtgaag aacgtcggcg
      421 gaaaaatgtt gctggtgcta atggcggtga ttggcattat cgctaaccca aacacagtgc
      481 cgcagaaaat tgacggtttt gaatggctgc caattaactt atatatcaat ggcgatactt
      541 tttactacat tctgtatggc atgttgggcc gcgctatagg gatgatggac acacagcata
      601 aagcactgtc gtgggtgagc gccgcgctgt ttgcgacggg ggtttttatt atctctcgcg
      661 ggacattata tgaattgcag tggcgcggaa attttgccga tacctggtat ctttactgtg
      721 ggccgatggt ttttatctgc gcaatcgcgc tattgactct ggttaaaaac acgctggata
      781 cgcgtaccat tcgcggactt ggcttaatct cccgccattc attgggtata tacggattcc
      841 acgccttgat tatccatgcg ctgcgcaccc ggggaattga gcttaaaaat tggccaatac
      901 tggatattat ttggatcttt tgcgcgacgt tggcagcgag tttgttactt tctatgctgg
      961 tacaacgaat cgacagaaac agattagtga gttaagtaaa agcccgttca cattggactg
     1021 accgggctta cgtgaattat tcgctgtatt cttcctgggt aacctgtgtt tcttttggcg
     1081 ggtttattcc ggcatcacga atattcttct gcaccgctac tgcaccaaac aagcttaaga
     1141 agaaagccag tccataaaaa cctttttcac tgagtaataa tgtcgcattc cacagaccta
     1201 ccatcagtaa tgcaacagag ataataaaga cagtcaggca ggtcatataa taaatggaag
     1261 tggtcggtat gccttcatac ttgtcccgaa cggtcttttg ataagacgca gcagaaaaca
     1321 gtcctaacac cagtacggca aaataatacc ctttttcatt taactgcatc tctgcattcc
     1381 atagccctaa cagataggta acgataccac caacgagagc tatccatgac acaatactaa
     1441 aggccggtga ataggttgat attttgttgt ccatcacagt attcctttca ttcctgaatg
     1501 tgtaagagct ttatgttgct cataccgagc gaaacaaacg catttgacca aacaagcaga
     1561 caaaaaaggc gatggggtag atcaccattt cataagcatt gataggtgca tttaaaacac
     1621 cgataaacaa gagaccgata gtgattaacg caactaactg gcagatatgg gtaaaaaaat
     1681 tatccagttg cccgagtttt tctgcgcgaa gatatgcata gttaccaaaa gttgctgtca
     1741 ttaacacgcc aagaaaatag ccttttccac ttagcaatgg gcatgagatc catagcccaa
     1801 ccagataaac cagcgccccg acaacaaata atagttttgc cacagttttt gaggttttca
     1861 taacgatctc catatctacc agcgatacat tacgagtaac caacgaaaga caaaactgaa
     1921 aaatgccatt aacaaatgat tttcagaata aattcatact aaatattaat tagtgagata
     1981 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     2041 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     2101 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     2161 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     2221 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     2281 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     2341 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     2401 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     2461 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     2521 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcgaccc
     2581 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     2641 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     2701 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     2761 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     2821 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcgg
     2881 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     2941 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     3001 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     3061 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     3121 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     3181 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     3241 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     3301 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     3361 agagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     3421 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     3481 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     3541 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctagcg
     3601 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     3661 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     3721 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     3781 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     3841 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     3901 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     3961 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     4021 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     4081 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     4141 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctggaa
     4201 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     4261 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     4321 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     4381 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     4441 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     4501 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     4561 atccacatcg tggaagcaat aaaatgacac atgtaacttg tggaaaaact caaatgcgac
     4621 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     4681 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt aacaggcggc
     4741 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     4801 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     4861 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     4921 atattgggca ctccctttca gttgctcaat tatgttattt cacactgcta ttgagataat
     4981 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     5041 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     5101 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     5161 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     5221 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     5281 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     5341 tacgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggct gaatctctcg
     5401 gtgcgaaagt atttgtacag tctgcaaatg gcaatggaga aacacaaatg tcgcagattg
     5461 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccatataac ggccaggtat
     5521 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     5581 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     5641 tgcaggcaaa agccctggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     5701 cgccggtaga taacaacgcc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     5761 acgttgattc cggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     5821 aaaacgcgct aaccgccaat aataacaaaa ttgatgctgt agttgcctca aacgatgcca
     5881 ccgcaggtgg ggcaattcag gcattaagcg cgcaaggttt atcagggaaa gtagcaattt
     5941 ccggccagga tgcggatctc gcaggtatta aacgtattgc tgccggtacg caaactatga
     6001 cggtgtataa acctattaca ttgctggcaa atactgccgc agaaattgcc gttgaattgg
     6061 gcaatggtca ggagccaaaa gcagatacct cactgaataa tggcctgaaa gatgtcccct
     6121 cccgcctcct gacaccgatc gatgtgaata aaaacaacat caaagatacg gtaattaaag
     6181 acggattcca caaagagagc gagctgtaag cgttacgccc cggcgcggag cgggggcgtg
     6241 atttctctcc atgccgcgtg aatgaattgg cttaggtgga gtcgttatgc cttatctact
     6301 tgaaatgaag aacattacca aaaccttcgg cagtgtgaag gcgattgata acgtcagctt
     6361 gcggttgaat gctggcgaaa tcgtctcact ctgtggggaa aatgggtctg gtaaatcaac
     6421 gctgatgaaa gtgctgtgtg gtatttatcc ccatggctcc tacgaaggcg aaattatttt
     6481 tgcgggagaa gagattcagg cgagtcacat ccgcgatacc gaacgcaaag gtatcgccat
     6541 cattcaccag gaattggccc tggtgaaaga attgaccgtg ctggaaaata tcttcctggg
     6601 taacgaaata acccacaatg gcattatgga ttatgacctg atgacgctac gctgtcagaa
     6661 gctgctcgca caggtcagtt tatccatttc acctgatacc cgcgttggcg atttagggct
     6721 tgggcaacaa caactggttg aaattgccaa ggcacttaat aaacaggtgc gcttgttaat
     6781 tctcgatgaa ccgacagcct cattaactga gcaggaaacg tcggttttac tggatattat
     6841 tcgcgatcta caacagcacg gtatcgcctg tatttatatt tcgcacaaac tcaacgaagt
     6901 caaagcgatt tccgatacga tttgcgttat tcgcgacggt cagcacattg gtacgcgtga
     6961 tgctgccgga atgagtgaag acgatattat caccatgatg gtcgggcgag agttaaccgc
     7021 gctttaccct aatgaaccac ataccaccgg agatgaaata ttacgtattg aacatctgac
     7081 ggcatggcat ccggttaatc gtcatattaa acgagttaat gatgtctcgt tttccctgaa
     7141 acgtggcgaa atactgggta ttgccggact cgttggtgcc ggacgtaccg agaccattca
     7201 gtgcctgttt ggcgtgtggc ccggacaatg ggaaggaaaa atttatattg atggcaaaca
     7261 ggtagatatt cgtaactgtc agcaagccat cgcccagggg attgcgatgg tacccgaaga
     7321 cagaaagcgc gacggcatcg ttccggtaat ggcggttggt aaaaatatta ccctcgccgc
     7381 actcaataaa tttaccggtg gcattagcca gcttgatgac gcggcagagc aaaaatgtat
     7441 tctggaatca atccagcaac tcaaagttaa aacgtcgtcc cccgatcttg ctattggacg
     7501 tttgagcggc ggcaatcagc aaaaagcgat cctcgcccgc tgtctgttac tcaacccgcg
     7561 cattctcatt cttgatgaac ctaccagggg tattgatatt ggcgcgaaat atgagatcta
     7621 caaattaatt aaccaactcg tccagcaggg tattgccgtt attgtcatct cttccgaatt
     7681 acctgaagtg ctcggcctta gcgatcgtgt actggtgatg catgaaggga aactaaaagc
     7741 caacctgata aatcataacc tgactcagga gcaggtgatg gaagccgcat tgaggagcga
     7801 acatcatgtc gaaaagcaat ccgtctgaag tgaaattggc cgtaccgaca tccggtggct
     7861 tttccgggct gaaatcactg aatttgcagg tcttcgtgat gattgcagct atcatcgcaa
     7921 tcatgctgtt ctttacctgg accaccgatg gtgcctactt aagcgcccgt aacgtctcca
     7981 acctgttacg ccagaccgcg attaccggca tcctcgcggt aggaatggtg ttcgtcataa
     8041 tttctgctga aatcgacctt tccgtcggct caatgatggg gctattaggt ggcgtcgcgg
     8101 cgatttgtga cgtctggtta ggctggcctt tgccacttac catcattgtg acgttggttc
     8161 tgggactgct tctcggtgcg tggaacggat ggtgggtcgc gtaccgtaaa gtcccttcat
     8221 ttattgtcac cctcgcgggc atgttggcat ttcgcggcat actcattggc atcaccaacg
     8281 gcacgactgt atcccccacc agcgccgcga tgtcacaaat tgggcaaagc tatctccccg
     8341 ccagtaccgg cttcatcatt ggccgcttgg cttaatggct tttgttggtt ggcaatggcg
     8401 cggaagaatg cgccgtcagg ctttgggttt acagtctccg gcctctaccg cagtagtcgg
     8461 tcgccaggct ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga atgattaccg
     8521 tggcgttccc actcctgttc tgctgctgac gttgctgtta ctcggcggaa tgtttatggc
     8581 aacgcggacg gcatttggac gacgcattta tgccatcggc ggcaatctgg aagcagcacg
     8641 tctctccggg attaacgttg aacgcaccaa acttgccgtg ttcgcgatta acggattaat
     8701 ggtagccatc gccggattaa tccttagttc tcgacttggc gctggttcac cttctgcggg
     8761 aaatatcgcc gaactggacg ctattgcagc atgcgtgatt ggcggcacca gcctggctgg
     8821 cggtgtggga agcgttgccg gagcagtaat gggggcattt atcatggctt cactggataa
     8881 cggcatgagt atgatggatg taccgacctt ctggcagtat atcgttaaag gtgcgattct
     8941 gttgctggca gtatggatgg actccgcaac caaacgccgt tcttgatttt gataaaaatt
     9001 ttctcaaagc cggttacgta ttaccggttt tgagtttttg catgattcag caggaaaaga
     9061 accatgttta ctaaacgtca ccgcatcaca ttactgttca atgccaataa agcctatgac
     9121 cggcaggtag tagaaggcgt aggggaatat ttacaggcgt cacaatcaga atgggatatt
     9181 ttcattgaag aagatttccg cgcccgcatt gataaaatca aggactggtt aggagatggc
     9241 gtcattgccg acttcgacga caaacaaatc gagcaagcgc tggctgatgt cgacgtcccc
     9301 attgttgggg ttggcggctc gtatcacctt gcagaaagtt acccacccgt tcattacatt
     9361 gccaccgata actatgcgct ggttgaaagc gcatttttgc atttaaaaga gaaaggcgtt
     9421 aaccgctttg ctttttatgg tcttccggaa tcaagcggca aacgttgggc cactgaacgc
     9481 gaatatgcat ttcgtcagct tgtcgccgaa gaaaagtatc gcggagtggt ttatcagggg
     9541 ttagaaaccg cgccagagaa ctggcaacac gcgcaaaatc ggctggcaga ctggctacaa
     9601 acgctgccac cgcaaaccgg gattattgcc gttactgacg cccgggcgcg gcatattctg
     9661 caagtatgtg aacatctaca cattcccgta ccggaaaaat tatgcgtgat tggcatcgac
     9721 aacgaagaac tgacccgcta tctgtcgcgt gtcgcccttt cttcggtcgc tcagggcgcg
     9781 cggcaaatgg gctatcaggc ggcaaaactg ttgcatcgat tattagataa agaagaaatg
     9841 ccgctacagc gaattttggt cccaccagtt cgcgtcattg aacggcgctc aacagattat
     9901 cgctcgctga ccgatcccgc cgttattcag gccatgcatt acattcgtaa tcacgcctgt
     9961 aaagggatta aagtggatca ggtactggat gcggtcggga tctcgcgctc caatcttgag
    10021 aagcgtttta aagaagaggt gggtgaaacc atccatgcca tgattcatgc cgagaagctg
    10081 gagaaagcgc gcagtctgct gatttcaacc accttgtcga tcaatgagat atcgcaaatg
    10141 tgcggttatc catcgctgca atatttctac tctgttttta aaaaagcata tgacacaacg
    10201 ccaaaagagt atcgcgatgt aaatcgtgag gtcatgttgt agcgatgatg agaattgtcg
    10261 gcgtcacatc agggaactca gcacagccac ctgatgcgag caaaagcctt atccgaccga
    10321 caaatcagat aaggctcaag gaaatgcaat tacatatgcg ccgcgattaa ccgttggtta
    10381 tcctggtaca ttgcgaacag gtagttatta taactcttcc ctttggtcga gtagcccttc
    10441 agcttgtgaa tcatcgctgt ggcagtcact tcctgatccg ctttacgcag ctgcgcacgc
    10501 gatttacgga acgaagagta agccgggtgc gtattcaggt tagtgacata ggcgctcacc
    10561 gattctttga cagaactaaa ctgtgagtac cctttcactt tacccggcgc attggtacaa
    10621 cgtcctttca tgcatttcat gccgaacaga ttgttattgt tgcgcgccag cttcgacgtt
    10681 ccccaaccgc tttctgctgc agccatcgtc gccaccatac tggtagggat aatgtctacg
    10741 cgttcaagca aggtattcca cgggattttt cgcgtattac cagaccactt caccttgtag
    10801 cgtttggcga tgtctttcag acgcgcacgc tcagcaggtg accattggcc ctgatactgt
    10861 tttgaaatca gccagttacg ttctgcagta atggctgcat tttggctggt aatgtaaggc
    10921 attacggtcc ggagaaacgc ctttttcctt ggtgttccgg aagggtattt tcgcaaatca
    10981 ggaagtgaac tactctttgc actattgcga gaatactctt gtttactgct tacctgttta
    11041 ttactagctt tagttaagtg ggacttttga ctcgctgttg ttgtgtgcgt cttcgctaac
    11101 acctcactcg aaaacaccag agtgagtaac ataagaatca tcgccccata tcgtcgtatg
    11161 ggagtcaaaa tcatcaggtc tcctggtcgg atttaatcat tccaacacct tatatttttc
    11221 acaaatttga gagttgaatc tcaaatcata tcaaaaatag ctgtcaagag caccccaagg
    11281 aatagtccaa atctgaaact atgtcacgag ttaacgattc agattggcgc taaatcgcag
    11341 aaaatgtggg gtttatcgca aaattcagcc gttttttgcg cgcgatcgct cacccttgct
    11401 tctcatcctg tggacttacc gctcagggat gagttttgtc tggcttatcg ctggcaaact
    11461 gtctgaaatc gcagcaataa ggactcatcc accatgaaac tcgcctcctg ttttctgaca
    11521 ctccttcctg gcttcgccgt tgccgccagc tggacttctc cggggttccc tgcctttagc
    11581 gaacagggaa cgggaacatt tgtcagccac gcgcagttgc ccaaaggtac gcgtccactc
    11641 acgctaaatt ttgaccaaca gtgctggcag cctgcagatg cgataaaact caatcagatg
    11701 ctttccctgc aaccttgtag caacacgccg cctcaatggc gattgttcag ggacggcaaa
    11761 tatacgctgc aaatagacac ccgctccggt acgccaacat tgatgatttc catccagaac
    11821 gccgccgaac cggtagcaaa cctggtccgt gaatgcccga aatgggatgg attaccgctc
    11881 acgctggatg tcagcgccac tttcccggaa ggagccgccg tacgggatta ttacagccag
    11941 caaattgcga tagtgaagaa cggtcaaata acgttacaac ccgctgctac cagcaacggt
    12001 ttactcctgc tggaacgggc agaaactgac acatccgccc ctttcgactg gcataacgcc
    12061 acggtttact ttgtgctgac agatcgtttc gaaaacggcg atcccagtaa tgaccagagt
    12121 tacggacgtc ataaagacgg tatggcggaa attggcactt ttcacggcgg cgatttacgc
    12181 ggcctgatca acaaactgga ttacctccag cagttgggcg ttaatgcttt atggataagc
    12241 gccccatttg agcaaattca cggctgggtc ggcggcggta caaaaggcga tttcccgcat
    12301 tatgcctacc acggttatta cacacaggac tggacgaatc ttgatgccaa tatgggcaac
    12361 gaagccgatc tacggacgct ggttgatagc gcacatcagc gcggtattcg tattctcttt
    12421 gatgtcgtga tgaaccacac cggctatgcc acgctggcgg atatgcagga gtatcagttt
    12481 ggcgcgttat atctttctgg tgacgaagtg aaaaaaacgc tgggtgaacg ctggagcgac
    12541 tggaaacctg ccgccgggca aacctggcat agctttaacg attacattaa tttcagcgac
    12601 aaaacaggct gggataaatg gtggggaaaa aactggatca gaacggatat cggcgattac
    12661 gacaatcctg gattcgacga tctcactatg tcgctggcct ttttgccgga tatcaaaacc
    12721 gaatcaacta ccgcttctgg tctgccggtg ttctataaaa acaaaacgga tactcacgct
    12781 aaagtcatcg aaggctttac acctcgcgat tacttaaccc actggttaag tcagtgggtc
    12841 cgcg
//
LOCUS       DAJTSL010000117        12784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTSL010000117 DAJTSL010000000
VERSION     DAJTSL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.117"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..455)
                     /locus_tag="OO433_002952"
     CDS             complement(<1..455)
                     /locus_tag="OO433_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6051353.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETG"
     gene            complement(485..1201)
                     /locus_tag="OO433_002953"
                     /pseudo
     CDS             complement(485..1201)
                     /locus_tag="OO433_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            1510..2286
                     /gene="exuR"
                     /locus_tag="OO433_002954"
     CDS             1510..2286
                     /gene="exuR"
                     /locus_tag="OO433_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6051354.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            2631..3293
                     /locus_tag="OO433_002955"
     CDS             2631..3293
                     /locus_tag="OO433_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6051355.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            3297..3680
                     /gene="mzrA"
                     /locus_tag="OO433_002956"
     CDS             3297..3680
                     /gene="mzrA"
                     /locus_tag="OO433_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6051356.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3827..4195
                     /locus_tag="OO433_002957"
     CDS             3827..4195
                     /locus_tag="OO433_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR6051357.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            4233..4538
                     /locus_tag="OO433_002958"
     CDS             4233..4538
                     /locus_tag="OO433_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6051358.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            4541..4945
                     /locus_tag="OO433_002959"
     CDS             4541..4945
                     /locus_tag="OO433_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6051359.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4935..5234
                     /locus_tag="OO433_002960"
     CDS             4935..5234
                     /locus_tag="OO433_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6051360.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            5420..5812
                     /locus_tag="OO433_002961"
     CDS             5420..5812
                     /locus_tag="OO433_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6051361.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSKFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5882..6785
                     /locus_tag="OO433_002962"
                     /pseudo
     CDS             5882..6785
                     /locus_tag="OO433_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            7078..7443
                     /locus_tag="OO433_002963"
     CDS             7078..7443
                     /locus_tag="OO433_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6051362.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7685..8041
                     /locus_tag="OO433_002964"
     CDS             7685..8041
                     /locus_tag="OO433_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6051363.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGVWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(8092..8984)
                     /gene="yhaJ"
                     /locus_tag="OO433_002965"
                     /pseudo
     CDS             complement(8092..8984)
                     /gene="yhaJ"
                     /locus_tag="OO433_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            9089..9790
                     /locus_tag="OO433_002966"
     CDS             9089..9790
                     /locus_tag="OO433_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6051364.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9813..9977
                     /locus_tag="OO433_002967"
     CDS             9813..9977
                     /locus_tag="OO433_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051365.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(10111..11421)
                     /locus_tag="OO433_002968"
     CDS             complement(10111..11421)
                     /locus_tag="OO433_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6051366.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNS
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(11449..12781)
                     /locus_tag="OO433_002969"
                     /pseudo
     CDS             complement(11449..12781)
                     /locus_tag="OO433_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT         3284 a         3246 c         3033 g         3221 t
ORIGIN      
        1 cctgtctcca gaacaccttt ggtggtttga gcaaacatca ggtttttagc cagagaaccg
       61 ttagttaacg catcggaagc aaagattttg gttgcggtaa aagtggttgc cgcatcagct
      121 ttaatttctt caccgcccca ggttactgtc agcggaacaa tcttgctagc atccaggctt
      181 tgaaccagtt gcgccgggga aactaactgc atttttacat ctttggtaac gtcgttagac
      241 cagattttgg tcatcaactg gtaagattgc agaccctgcc ccggcagata ttgcatttct
      301 actgctttcg gcaacgcggt gttatcggtc tgtgtcatat ccagagcggc atcaacgttg
      361 gccgttacag taatatcctt ttgtactgcc aatgcggagc ctgcaacaga aaacatcgct
      421 gcgaccagaa gagattttgc aaataccttt ttcattacta ttccttcgtt tattacaatg
      481 ataattaatg gaccgccagt tcaaactcgc cattatcttt ggtgatccag ttattatatt
      541 tgacaacgat tttgtcgtag ccagatatgc tttcaagatt tctttcctga ccagggaaga
      601 tgtttttatt tacggtctgt atcttacaat tttctttgct attattttta caataagcga
      661 tcgttaaaat ctttaagcgt tgattccctg cattaactaa tttttgggca gcatttactt
      721 ctaacttagc aataggttgc tgaggtggaa cactaacaac aatcccccaa attaagctaa
      781 cagttaactg catagagaaa tcttttttat ctgtggcaac gtcatcaata ctgggaaccg
      841 cttcaaattt aacgcgttaa tttttttctt tttgctctgg ttccattgca acaaagcgca
      901 cagttttgct actaccggct ggtaaagcga atttaggtgg cataacaacc agatgatttg
      961 catcaccgga tttaatctca acttcatttt cctgaggcgt cgatggatta tcgatacgga
     1021 atactgtcgc cttgatgtac tgaacttcat tacttttaga tattacgcga acgttgcctt
     1081 catcctgact attaatggaa acagccatag gataaacagt catatttgac tgtgccaaaa
     1141 aaggagcgaa cacgaataaa agcggcgcaa gccgtgataa gagattaatt ttcgccacca
     1201 tataaatttg atcttacaat attgtttcca aaaagttggt gtattagaga tgtagattta
     1261 ttgtaagtca atataaaaaa gcatttttag ttaatattaa atttaaaggc tgggaattaa
     1321 cttgctgaaa tttagtagct ttcacaatga cgatacttat tttttaggaa tttataaata
     1381 ttcacaatat taattcaata cattaattca atacattaat tcaatcacca aatttatata
     1441 tgaccaatgg catacccatg aataaagtgg tatgataact tcgtccgcat tccactccgg
     1501 gagacgcata tggaaatcac tgaaccacgc cgtttgtatc aacaacttgc cgctgacctg
     1561 aaagagcgca ttgaacaggg cgtctatctg gtgggtgata aactgcctgc cgaacgcttt
     1621 attgccgatg aaaagaacgt cagccgaacg gtagttcgtg aagccatcat catgctggaa
     1681 gttgaaggct atgtggaggt gcgtaaaggt tcaggtattc atgtggtttc caaccagccg
     1741 cgccatcagc aggcggctga caataatatg gaattcgcca attacggtcc gtttgagttg
     1801 cttcaggctc gccagctcat cgaaagtaat attgccgagt tcgcggcaac tcaggtaacg
     1861 aaacaagaca tcatgaaact gatggccatt caggaacagg cgcgcggcga acaatgcttc
     1921 cgtgattccg agtgggattt acagttccac attcaggtag ccctggcgac gcagaactcc
     1981 gccctggcgg ccatcgttga aaaaatgtgg acccagcgta gccataaccc gtactggaaa
     2041 aaactacacg aacacattga ttcccgtacc gttgataact ggtgtgatga ccacgatcaa
     2101 attctcaagg cgttaattcg caaagatccc catgctgcta agctggcaat gtggcagcat
     2161 ctggaaaaca ccaaaatcat gttatttaac gaaaccagcg acgatttcga gttcaatgcc
     2221 gaccgctatc tattcgccga aaacccggta gtgcatctcg atacggccac cagtggcagt
     2281 aaatgaaact cctgtccgac aggcgctgcg acgcgcctgt tcgcttcgtc ggtaagcgaa
     2341 aagtataaag tgtcagcctg tgtaaatcct ctcgccaccc tcccctgcat tcagcaaaat
     2401 cagactccac ggacatgtaa ttttgataac gaacaacgtt gacctttgtt acaattagat
     2461 tcaatttgaa tttatgtttt tgaatgcttt cttatctcac gatttaacag ggaatagttc
     2521 aggctgtgtt gatgtatcaa acccgcagaa cataccaaaa cagcaataac attgcggtag
     2581 tgcatctttt aaaaccagcg tggcgttaac cgattcacca ggaataatga atggaacttt
     2641 tgacccaatt gctgcaagcc ctgtgggcgc aggattttga aaccctggcc aatccatcga
     2701 tgattggcat gttgtatttt gtcttgtttg taattttgtt ccttgaaaac ggcttgcttc
     2761 cagcggcctt tttaccgggc gacagtttac tggtattggt cggcgtgttg attgcgaaag
     2821 gcgcgatggg ttacccgcaa acgattctgc tgctgaccgt tgccgccagc ctcggctgct
     2881 gggtcagcta tattcagggg cgatggctgg gcaatacccg caccgtacaa aactggctat
     2941 ctcatttacc cgcgcattat catcaacgcg cacaccatct ttttcataaa cacggtttat
     3001 cggcgctgtt aattggtcgc tttattgcgt ttgtcagaac actgctgccg acgattgccg
     3061 ggttatcagg gctgaataac gcgcgctttc agtttttcaa ctggatgagc ggtctgctgt
     3121 gggtattgat cctgacaact ctgggttaca tgctcggcaa aacgccggta tttttaaagt
     3181 acgaggacca gctgatgtca tgcctgatgc tgctcccggt ggtgctgctg gtgtttggcc
     3241 tggcaggttc tctggtcgtg ttatggaaaa agaaatatgg aaatcggggg taagggatgc
     3301 aaatacctcg catgtcgctt cgccagctag cctggtccgg cactgtttta cttctggtcg
     3361 gcacgctgtt gctggcctgg tccgcggttc gccagcaaga gtctacgctg gcgattcgtg
     3421 ccgttcatca aggcacaacg atgccagacg gtttttcgat ctggcatcac cttgacgctc
     3481 atggcattcc tttcaaaagt atcaccccca aaaacgacac tctgttaatt acatttgact
     3541 ccagcgacca gagcgccgcc gcaaaagcgg tcctcgacag aacattgccc catggctaca
     3601 tcattgcgca gcaggacaat aacagtcagg ctatgcagtg gctgacccgg ttacgggata
     3661 actctcatcg cttcggataa cttcctgtta tccgaaacat atcactcact ttggtgattt
     3721 caccgtaact gtctatgatt aatgaggcgg tggttaacac taccgcttcg ttgtctggac
     3781 aatcgttcct ttgtaacagg tccgaatcac aatggaaggt tcaagaatga aataccgcat
     3841 cgctttagct gtttctctct ttgctcttag tgccggtagt tatgccacta ccctgtgtca
     3901 ggaaaaggag caaaatatcc ttaaggagat cagctatgcc gaaaaacacc aaaaccagaa
     3961 tcgtattgac ggtctgaata aagccctgag tgaagtccgg gccaactgtt cagatagcca
     4021 gctgcgtgcc gatcatcaca agaaaatcgc aaagcagaaa gatgaggtgg cggaacgcca
     4081 gcaagattta gccgaggcga agcaaaaagg cgatgccgat aagattgcca aacgcgaacg
     4141 gaaactggca gaagcgcagg aagagctgaa aaagctggaa gcgcgcgact actaactcac
     4201 aatagtcact acttactcac ctggagaaaa ctatgtcgaa agaacacact acggaacatc
     4261 tgcgtgctga gttgaaatcc ctttccgata cgctggaaga ggtgcttagc tcatctggcg
     4321 agaagtcgaa agaagagttg agtaagattc gtagcaaagc ggagcaggca ctgaaacaga
     4381 gccgttatcg cctgggtgaa accggtgatg ccatcgccaa acaaacccgt gtcgcggcgg
     4441 cgcgtgctga tgagtatgtg cgtgaaaatc cgtggaccgg cgtgggaatt ggcgctgcaa
     4501 tcggtgtagt gctcggcgtt ctgctgtcgc gtcgttaatt atggcggaca ctcatcacgc
     4561 gcaagggccc ggtaaaagcg ttctgggcat cgggcagcga attgtttcca tcatggttga
     4621 aatggtggaa acacgtctgc ggctggcggt ggtggagctg gaagaggaaa aagctaatct
     4681 ctttcaactt ttactgatgc tgggcctgac gatgcttttc gctgcatttg gtcttatgag
     4741 cctgatggtg ctaattattt gggcggttga tccgcaatat cgcctgaatg cgatgattgc
     4801 caccaccgtg gtgttgctgc tactggcact gattggcggt atctggacgt tacgtaaatc
     4861 gcgtaaatcc accttgctgc gccatacacg ccatgagctg gcaaacgatc ggcaactgct
     4921 cgaggaggag tcccgtgagc agtaaagtcg aacgtgaacg acgtaaggcg caactgctta
     4981 gccagatcca gcaacaacgg ctggatcttt ccgccagtcg tcgtgaatgg ctggaggcaa
     5041 caggcgctta cgatcgtcgc tggaatatgc tgctaagtct gcgctcctgg gcgctggttg
     5101 gcagtagcgt gatggcgatc tggacgattc gccatcctaa tatgctggtc cgctgggcca
     5161 gacgcggttt tggcgtatgg agcgcctggc gtctggttaa aacgaccctc aagcagcaac
     5221 agcttcgcgg ttaacttccc tctggccgga gccattccgg ccctatcact caaatttttt
     5281 gaagattttt gacagttttc cttgctaaca atcatcattc accacgttta tgattctctc
     5341 catcgacagc aacgacgcta ataccgcgcc cttgcacaaa aaaacaatca gcagcctgag
     5401 tggcgcagtg gagagtatga tgaaaaaatt agaagatgtt ggtgtactgg tagcgcgcat
     5461 tttaatgccg attctgttta ttaccgcagg ctggggaaaa atcaccgctt actctggtac
     5521 ccaacaatat atggaagcaa tgggtgtccc gggttttatg ctgccgctgg tgattctgct
     5581 cgagttcggt ggtggtctgg caatcctgtt cggtttcctg actcgcacca cagccctgtt
     5641 tactgcgggc tttacgctgc tgacggcatt tttatttcac agcaagtttg ctgaaggcgt
     5701 caactcgctg atgttcatga aaaacctgac aatttctggc ggattcctgc tgctggcaat
     5761 taccggtccg ggcgcgtata gcatcgaccg cctgctgaat aaaaagtggt aagcacgcgc
     5821 tatacttaac gataaaaaac gaggaggaag ctcctcgttt ttgctattgg aggagagaaa
     5881 aatggggcaa ctgattgacg gcgtctggca tgacacctgg ccccactggc acaggcggtt
     5941 ttatcgcaga gaaagatcgt tatcatctct atgtttcact cgcctgcccg tgggcgcacc
     6001 gcacgctgat catgcgcaaa ctcaaaggac tggaaccgtt tatttccgtt tccgtagtga
     6061 acccgctgat gctggaaaac ggctggacct ttgatgacag ttttccggga gcaaccggag
     6121 atacgctcta tcaacatgaa tttctgtatc agctttatct ccacgccgat ccacactaca
     6181 gcggacgagt tactgttccc gtgctgtggg acaaaaagaa ccacaccatt gtcagcaacg
     6241 aatcagcaga aatcatccgc atgttcaata ccgcgtttga tgcgctgggc gcaaaagcgg
     6301 gtgattacta cccaccagcc ctgcaaacga aaattgacga acttaacggc tggatttatg
     6361 acaccgttaa caacggcgtg tataaagctg gttttgccac cagccagcaa gcctacgacg
     6421 aggcggtggc gaaagtgttt gaatcgctgg cgcgactgga gcagatttta ggccagcatc
     6481 gttacctgac cggcaaccag ctaaccgaag ccgatattcg cctgtggact acgctggtgc
     6541 gttttgatcc agtgtatgtg acccacttca agtgtgataa gcaccgcatc agcgattacc
     6601 tgaatctgta tggcttcctg cgcgatatct accagatgcc ggggatcgcc gaaactgtca
     6661 atttcgatca catccgtaat cattacttcc gcagccataa gaccatcaac cctacgggga
     6721 ttatttcaat tggtccgtgg caggatctcg atgaaccgca tggacgagat gttcgcttcg
     6781 gttaaaaaaa ggcaccttac gaggtgcctc tgataattag ctaagtgcat gttgttcatg
     6841 tcggatgcgg cgtgaacgcc ttatcaaacc tacaaaacgt ttatattcaa tatgttaaaa
     6901 tgaccgcgta ggccagataa gcgtagcgca tctggcgatt ttgcgcttgt catcagtctt
     6961 agcgcacctt cacggcactt tattaattcc ttgaacgaat atttactgcc atttcattca
     7021 cgtctattct taatttgctg ctttaaaaac aagtgattga gcaaaattga ggcaaaaatg
     7081 gactggtatc tgaaagtact aaaaaattat gtcggtttcc ggggtcgtgc gcggcgcaaa
     7141 gagtactgga tgtttattct ggtcaacatc atctttacgt tcgtgctggg gctgctggat
     7201 aaaatgttag gctggcaacg cgccggtggc gaaggcatcc tgacgacaat ctacggtatt
     7261 ctggtgtttt taccgtggtg ggcggttcag ttccgccgcc tgcacgacac cgaccgctcg
     7321 gcgtggtggg cactgttgtt cttaatcccg tttatcggct ggcttattat catcgtcttt
     7381 aactgccagg cgggtacgcc aggcgaaaac cgctttggac cagatccgaa actggaacct
     7441 taaaacaaat ctgctcgccg gatgtctgac tcatccggct tttatcatca tgttgatgaa
     7501 tgaataattt actaatgttt atttaaaata tttcaatgtc gttatataaa ttacctataa
     7561 aaaacaacca tgtatttatc ttaaatatca aaacctttat aacatccgaa aaagaaagat
     7621 acgaatagca tttacacaat acccggaata gactttcgtc acttttaatt aaagggatgt
     7681 ttttatgcag tggtatttag ccgtattaaa aaattatgtt ggtttctctg gtcgcgcacg
     7741 tcgtaaagag tactggatgt ttactctgat taacgccatc gtcggcgcta ttatcaatgt
     7801 cattcaattg attttaggtt tagagtttcc attcctttct ctcatttacc tggcagcaac
     7861 aatcattccg gtcattgctc tgtgtgttcg tcgtctgcac gatactgacc gttcaggtgt
     7921 gtgggcgctt ctctatctgg taccgatcat tggttggtta gttctgtttg tatttgcctg
     7981 tttagaaggc aattctggca gcaatcgtta cggaaacgat cctaagtttg gttcaaatta
     8041 atcttagaat tggggcgata tttcgcccct ttttattaac aataataaat attattttcc
     8101 gctaaaaagt ttgggaattt cccgcagaca ccaggatttc gcttccccca tactgtcacg
     8161 acgccaggcc ataataatat cgatctcgct ggtcgattcc gggctgacga cacgcaaccg
     8221 cccttccgca atatcttttt cgaccatcgg atacggcatc gtcgccacgc caagccccgc
     8281 cagtaatgcc tgacgtttat cttcaatcgt gctcaccgtt aagcgcggct gtttgtccag
     8341 cagctgtacg gtcaacaccg ggcgctcacg agcggtatcc gctaccgcaa ttccacgata
     8401 tttcacgcgc gtcacttcag ataacggttc cggctcctga tgaatcgggt gatcaggcgc
     8461 ggcaacgtag acgttcatta aggtatagag cttgcgcgag ttgatctccg acgaggaacg
     8521 aaaatgcata tccggcgcga taacgatatc cgcccgcccc tgctccagtc gttcccacgc
     8581 ccccgccagc acttcggtga tgattgccag ttgggtattg gcttttgccg ccagtttgtc
     8641 gattaacggg aaaaaggcag gtgtcggtac cagcgcctcg gtcacaatgg tgagatgcgt
     8701 ttcccaaccg cgcgcgaggg cttccgcatc ggtagtcagt ttatctgctg cttccagcaa
     8761 aacgcgccct cgctccagca acatcctccc gacattggtg aatttggtgc gatggcccga
     8821 gcggtcaaac agcaccacat ccagctcttc ttccagtttt tgcatggtgt agctaagtgc
     8881 ggaaggcacg cgtcccagct catccgccgc cgccgcaaaa ctgccccgac gatcgcatcc
     8941 ataacccgta gtgcttccag cgttaatgcc ctttctttgg ccatttcgtt ctcattcagg
     9001 aaatttgaac ataccgggca gaatatctgg ctaacaatgt agcgtccagc cccttaccat
     9061 aaaaggaagt aaagagaggt caagaattat gattactacc cgaactgcca ggcagtgtgg
     9121 acaagcagac tacggatggt tgcaggcccg gtatactttt tcctttggac actacttcga
     9181 cccgaaattg ttaggctatg cctccctgcg tgtgcttaac caggaagtgc tggccccagg
     9241 tgccgccttt cagccgcgaa cctatcccaa agtcgatatt ttaaatgtga ttctggatgg
     9301 agaagcagag tatcgcgaca gcgaaggcaa tcatgttcag gccagcgccg gtgaggcgtt
     9361 gctgctctct acccagccgg gtgtcagcta cagcgaacac aatctcagca aagacaaacc
     9421 gttaacacga atgcagcttt ggctggacgc ctgcccgcag cgagagaatc cgctgattca
     9481 aaaactggcg cttaatatgg gcaagcagca attaatcgcc tcgccagagg gtacgatggg
     9541 aagcctgcaa ttacgccagc aagtgtggct gcaccatatc gtgctcgaca aaggcgaaag
     9601 tgcgaatttc cagttgcatg ggccacgcgc gtatttgcaa tcgattcacg ggaaatttca
     9661 tgcgcttacg catcatgaag agaaagcggc gctgacctgc ggtgatgggg cgtttattcg
     9721 tgacgaggct aacattacgc tggttgccga ttccccactg cgcgctttgc tgatagattt
     9781 gcctgtctag ttgtttttac agggagatga tgatgagtaa aaaatcggcc aaaaagcgcc
     9841 agccagtgaa gcccgtggtg gcgaaagaac ctgctcgcac cgccaaaaat tttggctatg
     9901 aagagatgtt gagcgagctg gaagccatcg tcgcggatgc tgaaacgcgt ttagccgagg
     9961 atgaagctac cgcgtaaaga caggttgccg gatgcggcga aaacgcctta tccggcctac
    10021 aaaatccaac aacattaacg aattaaacaa cccgtaggcc ggataaggtg cgccagcatc
    10081 gcatccggca caatactcaa aacctggcgt ttatctggcc ttgctcgcca taatctcgat
    10141 aatctgccga tccgtttgct gcatcgaatg gcttgctaac gcacacaggt tggcaatcga
    10201 ctgctcaaca tcatgcgcca cgatcccttc attgccggtc acggcggtat catccagcgc
    10261 cattaacacc gctttccacg cagccgaagc actggtcgaa accttcatcg cgcagctgtt
    10321 cgacgcacca tcgcaaatca tgccgctgac atcgccgatc atactgctga tcgccatcga
    10381 gatagtttca taacgcccat ccaccagcca tgccatcccg gcggcggccc ccattgctgc
    10441 ggtcgttgcg gcacaaagtg cagacaaacg cggtaactgg ttatggatgt aaattgcgct
    10501 caaatgagaa agcatcagcg cacgcgccag tcgttcatca tccgctccga agtgttctgc
    10561 tacaaccacc acaggcattg ttgcggtgat cccctggtta cccgagccgg agttactcat
    10621 agccggaagc gtagcgccgc ccatacgcgc atcggatgcc gcgctggtac gaatcacaat
    10681 ggatgaagag agatctttcg ccagcaaacc gcgctcgcac tgtttttcca gcgtcgcgcc
    10741 aatatgcagc ccccacttac cgctcaaacc ttcctgcgat aacgcacaat ttagcttcgc
    10801 ggaatcgaga ataaagcgga tcgccgcaaa cgggacttca ttgacgaact tcaggatctc
    10861 agccagcgtc gttctggaaa gcaccgtcag cggagactct tgctcgccct ctgccacaca
    10921 cgcctgctgg gtaaacacca cactattgtg cgtctcaata tgcacaatgt tggtatgccc
    10981 gccgacgatg gtgacacacg cccacttctc accgttccag actttggcgc gtgagaagag
    11041 gatttcattg caaggttcct ggatcttaac ggagactttc cccgccgcca gcagtgcttt
    11101 ggcatcggca attgcctgcg cagttgcgtc tttcagcact tccagcccgg cgttggcatt
    11161 tccacctaac gcccccagcg ccgccgcaat cggcagcccc accattcccg tgccgggaac
    11221 ggtgacgccc agaccgttct tcatcagatt tggcgaaacc caggcttcta cacgttcaac
    11281 cggaccttcc agttctgccg cagcaaccgc cgccgccagc gccagtgaaa tcggttcagt
    11341 acatcccagc gccggtttta cttcctcctg aacggcgagg atgtaacgct gccataacgg
    11401 atttaaagtc gaatcaaaca tattaaaaac cttaaaattt caggtaaatc aagaaaatgc
    11461 caggaacgga gaaacacaaa gcaacaaacc agtgacgata atcaggtaca gagacatccc
    11521 tttgtatttg tgcaatgccg gtactttgta aaccagccac gccgggatca ggcaccctac
    11581 catgccgaaa atcgggctac agatagaggt gaagctcaac accggtgcgt tcagtacgat
    11641 ggcgctccag gccagcaaaa tggcgaaaat catgatgccg cgctgaacaa gattttcgtt
    11701 aatcttctcg gcaggcatct tgcgacgcag gatgttcatt acgatccctt gcgttgcttc
    11761 gcgaaagcct aaatagacgc caaagaatgc agtcatgaca gcaaagatat tgagaatgac
    11821 gctgaccact ttcacccacg ctgcgccgtc accgctaata aactgtgcgg caatcgccag
    11881 cgcggaaata ttctgctcgt aggcttttac cgcttcgtca tgtcccatcg ccagcgtgaa
    11941 cgacacggcg tagaaaaaga cggtgacaaa taaaatgcca aacgcgatat tcattgcccg
    12001 caatgcttta tgccgcgcca cttcaattga tttttcccgc gagcgataag agatcaccat
    12061 cgggcttaac gtctggataa acagaatcga cgtcagggta aacggcagcg taataatggc
    12121 gtttttcacc agcagcccca gcggcggtag cgaaccgacg ttatacagat gccacattcc
    12181 caccatcgac acgcccagcg cagcgactac cagcagcttg gtcagcacca tgccggtcga
    12241 aattttgaat aacaatttct cgccgcgtga agagatcgcc accagaatgc aaatcagtac
    12301 cagaccataa aagggactgt ctgacagcac cccttccgtc acgccgaagg tatgcagata
    12361 ggaagcacta tcgttggtga tggcggtgga atagacgaac atccaaatca ccagcattac
    12421 gaaatagagc gcacctaaca ggatgcccca gtttttacct aaataaccgc taatgacact
    12481 cgggtaatct ttacattctg tgtgattctg ccagcgtatt aataaacaac cgctgaaaca
    12541 gatacattgc cgggtaacca atcaccgatg agagcaaaaa tacccacaat cccatcaaac
    12601 cgacctgcac cgggagaaaa acaatccccg cgccaatcgc catcccgata ctcataatca
    12661 cccagccggt gtcagtactg tcgaatttga tcgcttctcg ccactcgctc tcactcattc
    12721 cagcacgacc cgccggggtc gaagcgtctg caatgacgcc tttattcgat gcaatttcca
    12781 taat
//
LOCUS       DAJTSL010000118        12595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTSL010000118 DAJTSL010000000
VERSION     DAJTSL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.118"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(17..523)
                     /gene="mug"
                     /locus_tag="OO433_002970"
     CDS             complement(17..523)
                     /gene="mug"
                     /locus_tag="OO433_002970"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR6051367.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            648..723
                     /locus_tag="OO433_002971"
     tRNA            648..723
                     /locus_tag="OO433_002971"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:681..683,aa:Ile,seq:cat)
     gene            complement(777..1541)
                     /gene="nfeF"
                     /locus_tag="OO433_002972"
     CDS             complement(777..1541)
                     /gene="nfeF"
                     /locus_tag="OO433_002972"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR6051368.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1829..2452
                     /gene="nfeR"
                     /locus_tag="OO433_002973"
                     /gene_synonym="yqjI"
     CDS             1829..2452
                     /gene="nfeR"
                     /locus_tag="OO433_002973"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6051369.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2606..4126)
                     /gene="aer"
                     /locus_tag="OO433_002974"
     CDS             complement(2606..4126)
                     /gene="aer"
                     /locus_tag="OO433_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR6051370.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSIIASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDVSADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4543..5922
                     /gene="ygjG"
                     /locus_tag="OO433_002975"
     CDS             4543..5922
                     /gene="ygjG"
                     /locus_tag="OO433_002975"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6051371.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLLAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5964..6296)
                     /locus_tag="OO433_002976"
     CDS             complement(5964..6296)
                     /locus_tag="OO433_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR6051372.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6515..7498
                     /gene="ebgR"
                     /locus_tag="OO433_002977"
     CDS             6515..7498
                     /gene="ebgR"
                     /locus_tag="OO433_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6051373.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7682..10774
                     /gene="ebgA"
                     /locus_tag="OO433_002978"
     CDS             7682..10774
                     /gene="ebgA"
                     /locus_tag="OO433_002978"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6051374.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLGNLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            10771..11220
                     /locus_tag="OO433_002979"
     CDS             10771..11220
                     /locus_tag="OO433_002979"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6051375.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11283..>12595
                     /locus_tag="OO433_002980"
     CDS             11283..>12595
                     /locus_tag="OO433_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6051376.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIF"
BASE COUNT         3012 a         3404 c         3168 g         3011 t
ORIGIN      
        1 ttttcggtgg cgttttttat cgcccacgca ctaccagcgc ctggtccagc tcgcgatacg
       61 cttcaaccag tttctctagt gaaacgcgac ttaaaccgct gggatttggc agcacccaaa
      121 tctgcgtcga accaatggtg agcgtttgtt tcccccactg tgcaccgcgc tggctgaatc
      181 cctgttcata tgcttgtttg cccagaatcg ccaacgcctg cggctgataa tcttcaattt
      241 tttcaatcag cttacgccca cctgcgtgca gctcctgctt tgaaacttca ttggcttgca
      301 ccgttggacg gtctaccagt ttggtgacgc cacaacgata atccagcaga tgctgtgcct
      361 cctgcggctt caactgacgg tcggtaaacc cggcctgata tatcaccttc cagaagcgat
      421 ttgctggatg agcaaaggga aaaccagtcc cggcggatga aagcccaggg ttgataccgc
      481 aaaacacgac ccgtaaccct ggagccaaaa tatcctcaac catatttact cctttctaac
      541 atcaccagga aaagtataac ggattgaaaa tacattgttt ataaaaacag caggcgcacg
      601 gtaatggctg gattgcgaca cggagttact ttataatccg ctaccatggc cccttagctc
      661 agtggttaga gcaggcgact cataatcgct tggtcgctgg ttcaagtcca gcaggggcca
      721 ccagatatag caaaggctga cgagaaatcg tcagcctttt tctttttata tctcagttac
      781 tttgcgtgcc agtaagccgc tgcacgtacc cgctgtgggt catactgttc cgcttcaaag
      841 cggcggctta aattcttaac gactttacct tcgccggtta tccagatgaa gtaatcatcg
      901 gcagggattt gcatctgcgc cagacgcgca tctaccgcct gctcatcatg tgccagccat
      961 tcgatattaa aaccatcaag gtgcgcgaga taatcctgac aggcgttatc ccgcacgcta
     1021 accagcgcac taacttgcgg tttaacggca agtttgctca acgtttccag gcggcggcgc
     1081 agtgcgggca ttccggattc atcgcagaca tacagctgat acgcgtaatc ttccggcacc
     1141 accagcgaac cgcgcggacc agccaccgta agtttatcgc ccggttgcgc ctgcatcgcc
     1201 cagccgctgg cgaccccacc gtcgtgaata aagaaatcga tcgccagttc atggcgtagt
     1261 tcgtcataca gcggcgtata gtcacgcgac ggtgggcgtg gtccttccgg ccagacgatg
     1321 ccctcttccg ttaccgttgg cggcacaaag tgagcgtcag gttgaggaaa gaagagtttg
     1381 ctgtgatcgt caaagccgcg cgatgtaaaa ccgtccagcg cctcgccgcc gaggacaatg
     1441 cgctgaaaac cggcgctgat gcgctcaacg cgtaacacag tcagttcacg gaagcgcaga
     1501 tcattgcgaa cgcgctgcgg gtagcggggg gagttattca ttgttatcgc cttcgtgatg
     1561 gtaatcagat atatctaaat aattttcgta aatgataatg attgttaatc atgataaatg
     1621 caagcgatta gtagaactga tatgtctata gtctgataag acgaaccgcc tcttctcagg
     1681 catcattact caacgccgga tgcggcgtga acgccttatc cggcctacgt gtgagatgag
     1741 tccacattta aaaatcattt ttattcttgc aagagtgctc atatcagata taaattagat
     1801 atatctaatc aagaaaaagg aggctgatat gagccatcat cacgaagggt gttgtaaaca
     1861 tgaaggccag ccacgccatg agggctgctg caaaggtgag aagtcagaac acgagcactg
     1921 cggacacggt caccagcatg aacacggtca atgctgcggt ggtcgccacg gtcgcggcgg
     1981 cggtcgtcgg caacgtttct ttggtcacgg tgaattgcgt ctggtgattc tggatattct
     2041 ctcgcgcgat gacagccacg gttacgaatt gattaaagcg attgagaatc taacccaggg
     2101 gaattacacc ccaagcccgg gcgtcatcta cccaacgctg gattttctgc aggagcagtc
     2161 gctgattacc atccgcgaag aggaaggtgg taagaagcag attgcgctga ccgaacaagg
     2221 cgcgcagtgg ctggaagaaa accgcgaaca ggtggagatg attgaagaac gcatcaaagc
     2281 gcgttgcgtt ggcgcggcgc tgcgccagaa cccgcaaatg aagcgggcgc tggataattt
     2341 taaagcggtg ctggatttac gcgtcaacca gagcgatatc agtgatgcac aaataaaaaa
     2401 gatcattgcg gtgatcgacc gcgccgcttt tgatattacg caactggatt aatcgccgca
     2461 tccgccagtg gcgcggtgca attgccggat gcgacgcttg acgcgcctta tccggcctac
     2521 acccactaca caccccgcag gcctgataag atgcgccagc atcgcatcag gcattgtgct
     2581 ccaaccgccg gatccggcat accgattaat gcagcaccgt caccgcgtct tccagtcggc
     2641 tggcgcggtg tttcaccatc gccgacacct gcgcactctc ttccaccagc tcagcatttt
     2701 tctgggtgat caggttaagc tcatccactg cacgggtcag gctggaaagc ccatcggcct
     2761 gttccagcgt tgaatggcta atctgggcga tcaactgggt gacgtttttc acctgtgcca
     2821 caatatcttc catcgtccgt ccggcggcgt gtacctgctg cgaaccggat tgcaccttat
     2881 cagcactgac atcaatcagc ttgcgaatat cgttggcagc attagcgctg cggctggcta
     2941 aatgacgcac ttcccctgcc accactgcaa aacctttgcc ctgttcgccg gcacgcgccg
     3001 cttccaccgc cgcattcagg gccagaatat tggtctgaaa cgcaatatcg ttaatcagcg
     3061 aagtaatggt gccaatgcgc tgggtactgt cggcgatatc gtccattgtc ttgatcaccg
     3121 tggtcatcgc ctccccaccc tgcactgccg cattactggc gatgatagaa agtttatcgg
     3181 cagccgacgc cgtggcagag ttctgtttca ccgacgccgc catttggttc atggtcgcca
     3241 ccgtttgctg aacgttatca actgtctgct gggtatgttc gttcagttca tcggtgcctt
     3301 tcgccagcgt ctcactgcca tttctgacac tggacacctg gcttgagaca tcgttaatta
     3361 gccaacggca catcaggcca agctgcccta ccgcgcgtaa tgtcagcccc agctcatcgc
     3421 tgcgattcag gtgctcaaca ctattacgct ctccggtcgc caccttcagt gcctgacggg
     3481 cgacattttc tatcgggcgc acaatctgcc attcaaaaca agcgcttgcc aacaatacca
     3541 ctaacacaca gaggaaatac gtcaccaccg gggcagcaac aaaccaaagc atggccgcca
     3601 gcaagataaa catcagggtc atcactccac gcgcccgcca gcgaagcggt aatgaaggca
     3661 gtttacccag ccagccttta cgcaccacca ggcctttatg aatacgctta ctggtacgtc
     3721 cggcgttcag cgctttgtac agcggctcca ccgccgcaat ctcttcatcc gtcgcccggg
     3781 tacgaatcga catatagcca ctgatttttc cctcgcgcac catcggtacc gcattggccc
     3841 gcacccaata atggtcgcca tttttgcggc gatttttcac gatgccgctc cagggctccc
     3901 cttttttcag ggtgaaccac atatccgcaa acgccgcttt tggcatatcc gggtgacgca
     3961 ccatgttgtg cggctgccct tgtaactctt gcaaggtaaa gccgctcacc tgcacaaaag
     4021 tgtcattagc atgagtgata tagctttgca gatcggtagt ggacatcaga gtggtatcgt
     4081 ccgccagcgg ggtattttgc tgggtgacat acggatgaga agacatgttc gcgtcctatg
     4141 caggttatat ggttgttaac ttcttgtcag agtttatgtc ggccccgctg cggttatctt
     4201 taaccgatta atttgattta gatcgcaatt tgcgattaaa gcacaaatct aattccttga
     4261 tttaaaatac tttcactctg ttactatacg aaaacgttaa ttatcttgcc caaaatcagg
     4321 caattatcgc cctgaaaacg tgcatttgca cagcaatcat caaatccata cccgacaaaa
     4381 accgtgcaaa ataacaacga atgttaacaa atagcattaa atactgcgca aatgataacc
     4441 acatttgtgt ttatcccgat tttcgcgatc gcagccggag tggcgcaatc cctgcaatac
     4501 ttaaatcggt atcatgtgat acgcgagcct ccggagcata ttttgaacag gttaccttcg
     4561 agcgcatcgg ctttagcgtg cagcgcccac gccctgaatc tcattgagaa gcgaacgctg
     4621 gatcatgagg agatgaaagc acttaaccga gaggtgattg aatacttcaa agagcatgtc
     4681 aatccggggt ttttagagta tcgcaaatct gttaccgccg gcggggatta cggagccgta
     4741 gagtggcaag cgggaagttt aaatacgctt gtcgacaccc agggacagga gtttatcgac
     4801 tgcctgggag gttttggaat tttcaacgtg gggcaccgta atccagttgt ggtttccgcc
     4861 gtacagaatc aacttgcgaa acaaccgctg cacagccagg agctgctcga tccgttacgg
     4921 gcgatgttgg cgaaaaccct tgctgcgcta acgcccggta aactgaaata cagcttcttc
     4981 tgtaatagcg gcaccgagtc cgttgaagca gcgctgaagc tggcgaaagc ttaccagtca
     5041 ccgcgcggca agtttacttt tattgccacc agcggcgcgt tccacggtaa atcacttggc
     5101 gcgctgtcgg ccacggcgaa atcgaccttc cgcaaaccgt ttatgccgtt actgccgggc
     5161 ttccgtcatg tgccgtttgg caatatcgaa gccatgcgca cggctcttaa cgagtgcaaa
     5221 aaaaccggtg atgatgtggc tgcggtgatc ctcgaaccga ttcagggtga aggtggcgta
     5281 attctgccgc cgccgggcta tctcaccgcc gtacgtaagc tatgcgatga gttcggcgca
     5341 ctgatgatcc tcgatgaagt acaaacgggc atggggcgca cgggcaagat gttcgcctgc
     5401 gagcatgaga acgtacagcc ggatatcctc tgccttgcca aagcgctcgg cggcggcgtg
     5461 atgccgattg gcgcgaccat cgccactgaa gaggtgtttt cagttctgtt cgacaaccca
     5521 ttcctgcata ccaccacctt tggcggcaac ccgctggcct gtgcggcggc gctggcgacc
     5581 atcaatgtgt tgctggagca gaacttactg gctcaggctg agcaaaaagg cgatatgttg
     5641 ctggacggtt tccgtcaact ggcgcgggaa tatcccgatc tggtacagga agcgcgtggt
     5701 aaagggatgt tgatggcaat tgagtttgtt gataacgaaa tcggctataa ctttgccagc
     5761 gagatgttcc gccagcgcgt actggtggcc ggaacgctca ataacgccaa aacgatccgc
     5821 attgaaccgc cactgacact gaccattgaa cagtgtgaac tggtgatcaa agcggcgcgt
     5881 aaggcgctgg cggccatgcg agtaagtgtc gaagaagcgt aatatcaaat cggatggcga
     5941 tacgacgtcg ccatccgatc tttttatacg acgcgtacgc ccgcaggcat catccgctcc
     6001 ggcgttaaca acacgctttc actgccgtca tcggtttccg cacacaacag catgcattcc
     6061 gacgtttcac cacgcatttt cgctttttgc agattgcata agaccaccac cgttttcccc
     6121 atcagctctt cttcgctgta gtacggcacc aggctggtca cggtttgcag tgttttttcc
     6181 cccacatcaa cctgtacgat gtacagcttg tcggcgtttt catggcgttt cacttccaca
     6241 atctttccga cgcgcatttc cagacgtgca aaatcagcgt aagccacggt ttccattgtt
     6301 tccttccttc gggtaaaatt ttactaaact atagcaaaag tttttctcaa tcctgtaggc
     6361 taaaaatgga gaatgcaggc gtgatcacat tcgtgagccg ctgtgttacc gttacagcgt
     6421 caaagaaacg cgctttattt actgaaaaca ggtgacccga taagtacttc ctctacaatg
     6481 ggggcgcaca tcagggaaag taaaaaaggt aaacatggca acactaaaag acatcgcaat
     6541 cgaagctggc gtatccctgg cgacagtatc cagggtctta aatgacgatc cgacattgaa
     6601 tgtgaaagaa gagacgaaac atcgcattct cgagatcgcc gaaaagctgg agtacaagac
     6661 cagtagtgcc cgcaaactcc agacaggtgc agtcaaccaa caccatattc tggctatcta
     6721 cagctaccaa caggagctgg agatcaacga tccttactat ctggcgatcc gccacggcat
     6781 tgaaactcag tgcgaaaagc tgggcattga gctcaccaac tgttatgaac acagcggatt
     6841 accagacatt aaaaacgtca ccggtatttt aattgtcggc aaacccacgc ccgcccttcg
     6901 cgccgccgcc tgtgcgttga ccgacaatat ctgttttatc gactttcacg aacccggcag
     6961 cggttacgat gcggtggata tcgatctggc acgcatcagt aaagaaatca tcgacttcta
     7021 tatcaatcag ggcgttaatc gtattggttt tattggcggt gaagatgagc caggcaaagc
     7081 ggatattcgt gaggtcgcct ttgcggaata tggccgactg aaacaagtgg tacgcgaaga
     7141 ggatatctgg cgcggaggct tttccagttc gtcgggttat gaactggcaa aacagatgct
     7201 ggcgcaggaa gactatccga aggcactgtt tgttgcttcc gattccattg ctatcggcgt
     7261 actgcgggca attcatgaac gaggcctgaa catcccacag gatatttcgc ttatcagcgt
     7321 taacgatatc cccaccgcgc gatttacctt tccgccgctc tccaccgtgc gcatccattc
     7381 cgagatgatg ggaagtcagg gcgttaacct ggtgtatgaa aaagcccgcg atggtcgcgc
     7441 gctgccgctg ttagtcttcg ttcccagcaa attaaaactg cgcggcacga cccgttaaat
     7501 ccccttacac actgtccggc aatcgttttt gccggacagt gctgccgttt attttcgtga
     7561 tccagttaaa gtaaatgcat ttatctgcta ctttttagta aaaattttac tgaactcccc
     7621 agcaattaca caaactacca tcaccatgaa tggttccgat ttctctctac cgggaggccc
     7681 tatgaatcgc tgggaaaaca ttcagctcac ccacgaaaac cgacttgcgc cgcgtgcgta
     7741 ctttttttca tatgattctg ttgcgcaagc gcgtaccttt gcccgcgaaa ccagcagcct
     7801 gtttctgccc ttaagcggtc agtggaattt ccactttttt gaccatccgc tgcaagtacc
     7861 agaagccttc acctctgagt taatggctga ctgggggcat attaccgtcc ccgccatgtg
     7921 gcaaatggaa ggtcacggca aactgcaata taccgacgaa ggttttccgt tccccatcga
     7981 tgtgccgttt gtccccagcg ataacccaac cggtgcctat caacgtattt tcaccctcag
     8041 cgacggctgg cagggtaaac agacgctgat taaatttgac ggcgtcgaaa cctattttga
     8101 agtctatgtt aacggtcagt atgtgggttt cagcaagggc agtcgcctga ccgcagagtt
     8161 tgacatcagc gcgatggtta aaaccggcga caacctgttg tgtgtgcgcg tgatgcagtg
     8221 ggcggactct acctacgtgg aagaccagga tatgtggtgg tcagcgggga tcttccgcga
     8281 tgtttatctg gtcggaaaac acctaacgca tattaacgat ttcactgtgc gtaccgactt
     8341 tgacgaagcc tattgcgatg ccacgctttc ctgcgaagtg gtgctgggaa atctcgccgc
     8401 ctcccctgtc gtcacgacgc tggaatatac cctgtttgat ggcgaacgcg tggtgcacag
     8461 cagcgccatt gatcatttgg caattgaaaa actgaccagc gccagctttg cttttactgt
     8521 cgaacagccg cagcaatggt cagcagaatc cccttatctt taccatctgg tcatgacgct
     8581 gaaagacgcc aacggcaacg ttctggaagt ggtgccacaa cgcgttggct tccgtgatat
     8641 caaagtgcgc gacggtctgt tctggatcaa taaccgttat gtgatgctgc acggcgtcaa
     8701 ccgtcacgac aacgatcatc gcaaaggccg cgccgttgga atggatcgcg tcgagaaaga
     8761 tctccagttg atgaagcagc acaatatcaa ctccgtgcgt accgctcact acccgaacga
     8821 tccgcgtttt tacgaactgt gtgatatcta cggcctgttt gtgatggcgg aaaccgacgt
     8881 cgaatcgcac ggctttgcta atgtcggcga tatcagccgt attaccgacg atccgcagtg
     8941 ggaaaaagtc tacgtcgagc gcattgttcg ccatatccac gcgcagaaaa accatccgtc
     9001 gatcatcatc tggtcgctgg gcaatgaatc cggctatggc tgtaacatcc gcgcgatgta
     9061 ccatgcggcg aaagcgctgg atgacacgcg actggtgcat tacgaagaag atcgcgatgc
     9121 tgaagtggtc gatattattt ccaccatgta cacccgcgtg ccgctgatga atgagtttgg
     9181 tgaatacccg catccgaagc cgcgcatcat ctgtgaatat gctcatgcga tggggaacgg
     9241 accgggcggg ctgacggagt accagaacgt cttctataag cacgattgca ttcagggtca
     9301 ttatgtctgg gagtggtgcg accacgggat ccaggcacag gacgaccacg gcaatgtctg
     9361 gtataaattc ggcggcgact acggcgacta tcccaacaac tataacttct gtcttgatgg
     9421 tttgatctat tccgatcaga cgccgggacc gggcctgaaa gagtacaaac aggttatcgc
     9481 gccggtaaaa atccacgcgc gggatctgac tcgcggcgag ttgaaagtcg aaaataaact
     9541 gtggtttacc acgcttgatg actacaccct gcacgcagag gtgcgcgccg aaggtgaaac
     9601 gctcgcgacg cagcagatta aactgcgcga cgttgcgccg aacagcgaag cccccttgca
     9661 gatcacgctg ccgcagctgg acgcccgcga agcgttcctc aacattacgg tgaccaaaga
     9721 ttcccgcacc cgctacagcg aagccgggca ttctatcgcc acttatcagt tcccgctgaa
     9781 ggaaaacacc gcgcagccag tgcctttcgc accaaataat gcgcgtccgc tgacgctgga
     9841 agacgatcgt ttgagctgca ccgttcgcgg ctacaacttc gcgatcacct tctcaaaaat
     9901 gagtggcaaa ccgacatcct ggcaggtaaa tggcgagtcg ctgctgaccc gcgagccaaa
     9961 gatcaacttc ttcaagccga tgatcgacaa ccacaagcag gagtacgaag ggttgtggca
    10021 gccgaatcat ttgcagatca tgcaggaaca tctgcgcgac tttgccgtag aacagagcga
    10081 tggtgaagtg ttgatcatca gccgcacagt tattgcaccg ccggtgtttg acttcgggat
    10141 gcgctgtacc tacatctggc gcatcgctgc cgatggccag gttaacgtgg cgctttccgg
    10201 cgagcgttac ggcgactatc cgcacatcat tccgtgcatc ggtttcacca tggggattaa
    10261 cggcgaatac gatcaggtgg cgtattacgg tcgtggaccg ggcgaaaact acgccgacag
    10321 ccagcaggct aacatcatcg atatctggcg cagcaccgtc gatgccatgt tcgagaacta
    10381 tcccttcccg cagaacaacg gcaaccgtca gcatgtccgc tggacggcac tgactaaccg
    10441 ccacggcaac ggtctgctgg tggttccgca gcgcccaatt aacttcagcg cctggcacta
    10501 tacccaggaa aacatccacg ctgcccagca ctgtaacgag ctgcagcgca gtgatgacat
    10561 caccctgaac ctcgaccacc agctacttgg cctcggctcc aactcctggg gcagcgaggt
    10621 gctggactcc tggcgcgtct ggttccgtga cttcagctac ggctttacgt tgctgccggt
    10681 ttctggcgga gaagctaccg cgcaaagtct ggcgtcgtat gagttcggcg cagggttctt
    10741 ttccacgaat ttgcacagcg agaataagca atgaggatca tcgataactt agaacagttc
    10801 cgccagattt acgcctctag caagaagtgg caacgctgcg ttgaagcgat tgaaaatatc
    10861 gacaacattc agcctggcgt cgcccactcc atcggtgact cattgactta ccgcgtggag
    10921 acagactccg cgaccgatgc gctatttacc gggcatcgac gctattttga agtgcattac
    10981 tacctgcaag ggcagcaaaa aattgaatat gcgccgaaag agacattaca ggtagtggaa
    11041 tattatcgtg atgaaactga ccgtgaatat ttaaaaggct gcggagaaac cgttgaggtc
    11101 cacgaagggc aaatcgttat ttgcgatatc catgaagcgt atcggtttat ctgcaataac
    11161 gcggtcaaaa aagtggttct caaagtcacc atcgaagatg gttatttcca taacaaataa
    11221 caactacggc ggcaaaagga gtttgccgcc accgctaccc tacacatttt cggagatgtg
    11281 ttatgtctga taccaaacgt aatacaatcg gcaaattcgg cttgctctcg ctgacttttg
    11341 ctgccgttta cagctttaac aacgttatca ataataatat tgagcttgga ctggcctcgg
    11401 caccgatgtt tttcctcgcg acgatttttt attttattcc cttctgtctg atcatcgcag
    11461 aatttgtttc gttaaataaa aactcagaag ccggtgtcta cgcgtgggta aaaagttcgc
    11521 tgggcggacg ttgggcattt attactgcct atacctactg gttcgtaaac ctgttctttt
    11581 tcacctcact gttgccgcgc gttattgctt atgcttcgta tgccttcctc ggatacgaat
    11641 atattatgac gccggttgcc accaccatta tcagtatggt gctgttcgcc ttctccacct
    11701 gggtttccac caacggggcg aaaatgttgg ggccaattac ctccgtcact tcaacgctga
    11761 tgctgctgtt aacgctctcc tacattttac tggcaggtac ggcgctggtt ggcggcgtac
    11821 agcctgctga ccccatcacc gttgacgcga tgatcccgaa cttcaactgg gcgttcctcg
    11881 gcgttaccac ctggatcttt atggccgcag gtggcgcgga gtccgtcgct gtgtacgtta
    11941 acgacgtcaa aggcggttcg aaatcgttcg ttaaagtgat catcctcgcc gggattttta
    12001 tcggcgtact gtattccgtc tcctcggtgc tgattaacgt cttcgtcagc agcaaagagt
    12061 tgaaatttac cggcggatcg gtgcaggtat tccatggcat ggcggcgtat tttggtctac
    12121 cggaagcgtt gatgaatcgc tttgtcggtc tggtgtcctt taccgcgatg ttcggttccc
    12181 tgctgatgtg gaccgcaacg ccggtgaaaa ttttcttctc cgaaatcccg gaaggcatct
    12241 ttggtaagaa aaccgtcgaa ctgaacgaaa acggcgttcc ggcgcgcgca gcgtggatcc
    12301 agttcctgat cgtcatcccg ctgatgatta tcccgatgct cggttccaat accgtgcagg
    12361 atctgatgaa tacaattatt aatatgaccg ccgcagcgtc catgcttccg ccgttattca
    12421 tcatgctggc ttacctgaat ttacgcgcca aattagatca cctgccacgc gatttccgta
    12481 tgggctcccg ccgcaccggt attatcgttg tttcaatgct gattgcgata tttgccgtag
    12541 ggtttgtcgc ttcgacattc ccgactggcg cgaatattct gaccatcatt tttta
//
LOCUS       DAJTSL010000119        12581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTSL010000119 DAJTSL010000000
VERSION     DAJTSL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.119"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            222..1319
                     /gene="mltA"
                     /locus_tag="OO433_002981"
     CDS             222..1319
                     /gene="mltA"
                     /locus_tag="OO433_002981"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6051377.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKSTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1396..2202
                     /gene="tcdA"
                     /locus_tag="OO433_002982"
     CDS             1396..2202
                     /gene="tcdA"
                     /locus_tag="OO433_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6051378.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSIGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2253..2696)
                     /gene="csdE"
                     /locus_tag="OO433_002983"
     CDS             complement(2253..2696)
                     /gene="csdE"
                     /locus_tag="OO433_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6051379.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVLGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2696..3901)
                     /gene="csdA"
                     /locus_tag="OO433_002984"
     CDS             complement(2696..3901)
                     /gene="csdA"
                     /locus_tag="OO433_002984"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6051380.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALSQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSHSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4093..4320
                     /locus_tag="OO433_002985"
     CDS             4093..4320
                     /locus_tag="OO433_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6051381.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4671..5588
                     /gene="gcvA"
                     /locus_tag="OO433_002986"
     CDS             4671..5588
                     /gene="gcvA"
                     /locus_tag="OO433_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6051382.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5607..6002
                     /locus_tag="OO433_002987"
     CDS             5607..6002
                     /locus_tag="OO433_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6051383.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5995..7095
                     /gene="rlmM"
                     /locus_tag="OO433_002988"
     CDS             5995..7095
                     /gene="rlmM"
                     /locus_tag="OO433_002988"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6051384.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENSGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7139..7870)
                     /gene="fucR"
                     /locus_tag="OO433_002989"
     CDS             complement(7139..7870)
                     /gene="fucR"
                     /locus_tag="OO433_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6051385.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICYELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7928..8350)
                     /gene="fucU"
                     /locus_tag="OO433_002990"
     CDS             complement(7928..8350)
                     /gene="fucU"
                     /locus_tag="OO433_002990"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6051386.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8352..9770)
                     /gene="fucK"
                     /locus_tag="OO433_002991"
     CDS             complement(8352..9770)
                     /gene="fucK"
                     /locus_tag="OO433_002991"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR6051387.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERTHAWFFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILIVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     SADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9767..11512)
                     /gene="fucI"
                     /locus_tag="OO433_002992"
     CDS             complement(9767..11512)
                     /gene="fucI"
                     /locus_tag="OO433_002992"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6051388.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEVLQERL"
     gene            complement(11545..>12581)
                     /gene="fucP"
                     /locus_tag="OO433_002993"
     CDS             complement(11545..>12581)
                     /gene="fucP"
                     /locus_tag="OO433_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6051389.1"
                     /translation="ITGLFLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAA
                     NPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQ
                     LSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRL
                     ARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGR
                     FTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFS
                     LGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIF
                     ARFRSQTATN"
BASE COUNT         3013 a         3146 c         3331 g         3091 t
ORIGIN      
        1 gctccacccc gcgtccgtgg atgcgcacta tactccgctc gcgttttcat gcaacctttt
       61 ttttaccccg atcacggatt tagcatgatt ttgaacaaaa acaaaccact aaagcctatt
      121 ttttgtgcaa aatttgcgtc agggagaggc tgctcatttc attaaacgcg gcggtttgtt
      181 atcttcgttg cgccttattt tttaacctga agaagagaac aatgaaagga cgttgggtaa
      241 agtaccttct tatgggcacg gttgtggcaa tgcttgccgc ctgctcttcc aaatcaaccg
      301 atcgcggaca gcaatataaa gacgggaaat ttacccagcc tttctctctg gtgaaccagc
      361 cagatgccgt tggcgcgccg attaacgccg gtgattttgc cgagcaaatt aaccatatcc
      421 gtaattcgtc accgcgtctg tatggcaacc agagtaatgt ttataacgcg gtgcaagagt
      481 ggctgcgcgc aggcggtgat acccgcaata tgcgccagtt cggcattgat gcctggcaga
      541 tggaaggtgc cgacaactat ggtaacgtgc agtttacagg ctattacacg ccggtaattc
      601 aggcgcgcca tacccgccag ggcgagttcc agtatcctat ttaccgtatg ccgccaaaac
      661 gtggtcgtct gccgtctcgt gcggagatct acgcgggggc attgggcgat aaatacatcc
      721 tcgcttacag caactctctg atggataact tcattatgga tgtgcagggt agtgggtata
      781 tcgactttgg tgatggcagt ccgcttaact ttttcagcta tgcagggaaa aacggtcatg
      841 cctatcgcag cattggtaag gtgctgatcg accgtggcga agtgaaaaaa gaagatatgt
      901 cgatgcaggc gattcgtcat tggggcgaaa cacacagtga agccgaggtt cgcgagctgc
      961 tggaacagaa cccgtctttc gtcttcttta aaccgcaatc ttttgcaccg gtgaaagggg
     1021 caagtgcggt gccgctggtt ggccgtgctt ctgttgcctc cgatcgttca attattccgc
     1081 caggtactac cttgctggca gaagtgccgc tgctggataa taacggcaaa tttaacggtc
     1141 agtacgaatt gcgtctgatg gtggcgctgg atgtcggtgg cgcaatcaaa ggccaacact
     1201 tcgatatcta tcaagggatc gggccggaag ccggacatcg cgcaggttgg tacaaccact
     1261 atggacgtgt ctgggtgctg aaaaccgccc cgggcgcagg taacgtcttt agcggctgat
     1321 gtgttattct gtataccgtt tttacggata cgacagggtg agggataacc tcaccctttt
     1381 taattctgag gtgctatgtc tgtggtaatt agtgatgcat ggcgtcagcg ttttggtggc
     1441 acagcgcgtc tgtatggtga aaaagcgttg caactgtttg ctgacgcgca tatttgtgtg
     1501 gttggtatcg gtggtgtcgg ttcctgggcg gcggaagcgc tggcgcgcac ggggattggc
     1561 gcaatcacgc ttatcgatat ggatgatgtg tgcgtcacca ataccaatcg gcaaattcac
     1621 gctctgcgcg ataacgtcgg gctggcaaaa gcggaagtca tggcggagcg cattcgccag
     1681 attaacccgg agtgccgtgt aacggtggtg gatgatttcg tgacgccgga taacgtagcg
     1741 caatatatga gtgccggtta ttcgtacgtg attgatgcca ttgatagtgt acgacccaaa
     1801 gcggcgctga ttgcttattg tcggcgcaat aaaatcccgc tggtcacgac cggtggcgcg
     1861 ggtgggcaga ttgacccgac gcagattcag gttaccgatc tggcgaaaac gattcaggac
     1921 ccgctggcgg cgaagttgcg cgagcgcctg aaaagcgatt ttggcgtagt gaaaaacagt
     1981 ataggtaagc tcggcgtgga ttgcgtgttt tctactgaag cgctggtgta cccgcagtca
     2041 gacggtacgg tgtgtgcgat gaaagccacg gcagaagggc cgaagcggat ggattgtgca
     2101 tctggatttg gcgcggcaac gatggtgacc gccacctttg ggtttgttgc ggtttctcat
     2161 gcgctgaaga agatgatggc gaaagcagcg cgtcagggtt aaacaatcag gtcggatgcg
     2221 acgcgaacgt cttatccgac ctggttctgt gcttaaacct gcttcgccgc agcgataatt
     2281 gcctcgctta acgcattcaa cccctggctg cgtgaggcgc taagctgccc acgcaatccc
     2341 agctcatcaa acaatgccag cggcgactgt gcctgtaatt cggcggcggt tttcccctca
     2401 acggcagtca acaacaccgc caacaggccg agcacaatgc ggccttcgct gtcgccaaag
     2461 aagtgcattt taccgttttc agccacggta tatcccagcc agacgcggtt ttcgcatccg
     2521 gcaatctctt tagcctgcgc ttttaactcg tctggcaaag ccggaagctg tttccccagc
     2581 atgatcaact ggcgatattt atcttcccat tgcgtcaacg gtgcgaaggt attgcgtaac
     2641 gtttctgcgg ttacggttgt gccgaacgga tgtccggcga attgcgggtt tgtcattaat
     2701 ccaccaataa ttccagcgcg cggtcaacgg cattcaccag cgcatccaca tcactctttg
     2761 tattatatgg cgcaaaagag gcgcgcagtg tgccggttac gcctaattct gccagtagcg
     2821 gctgagcaca atgttgcccg gcccgcaagg caataccgta ctccgccagt agcgtcacca
     2881 tatcgctgtg atgaacgcca gcaaaatcaa aggccagcag gctggaatcc tggcagcgga
     2941 atgaacgaaa gcctggacgt ttcgccagcg catcttccgc cagcgttgct aagctatggc
     3001 tccagttttc ggcctggttg atatcgtaat ctgccagcca ttccagcgcc gcgcttaatc
     3061 ctatgacacc agccacattt ggcgttcctg cttccagttt ccacggcgca gattgagtcg
     3121 tgaagccgtc aaaactcact tcgtgaatca ttttgccgcc acccagccag ggcgacatcg
     3181 cttccagcag ttctgatttg ccatacagca cgccgatacc cgtcgggcca tacagtttgt
     3241 gaccagagaa agcatagaaa tcgatatcca gttgctgaac atccgcgggg aaatgcaccg
     3301 ccccctgagc accatcaacc atcaccacca tccctgctga atgagcaaag gtaatcgctc
     3361 gcgccagatc cgggcaaccg ccagtaacgt tcgacatctg actcaacgcc agaatccgac
     3421 tacggggagt aatcagttct ggcaacaaat cgacatccgg cagtcgctgc gcattaagcg
     3481 gcaatttcac cactttggct ccagtttgtt gggcgaccat cagccagggg acgaggttgg
     3541 cgtggtgttc tgccacgctg acaataatct catcgcccgg ttgcagacgc ggacgcgcat
     3601 agcattgtgc caccatgttg atggattcag tggtgccgcg cgtccagacg atagttttat
     3661 catccggtgc attcagtaat tgcgccactt tctctcgtgc agcttcataa cgcgcggtca
     3721 ggcgttgggc ttcggcaaac tggctgcgat ggacgtttcc ggcgctcagg ctgtaaaact
     3781 gtcgagtggc ttcaaccacg gcttcaggtt taagcgcggt tgcggcgctg tcgagataga
     3841 cgcccgcatc ctgtagtgcg ggaaactggg cgcgaaactg cgcgggatta aaaacgttca
     3901 tggtactcct cggcttgatg caccgatcgt gacgcaaaat tcactttgat acaaggagag
     3961 tgctaaccat cagaattatc tggatatcct ggcgaagccg cgtaagtaac atatgctgaa
     4021 tagtgttagg cgttaatttt agcctgttaa tcaatactat taagatttat taatcgcttt
     4081 aaggagaaga tgatgaaaaa gactgccgca attatttctg cctgtatgct gacttttgcc
     4141 ctgagcgcct gttccggttc gaactatgtg atgcacacca atgacggacg taccatcgtc
     4201 tctgacggca aaccacagac tgataacgat accggtatga tttcgtataa agacgctaat
     4261 ggcaacaaac agcagatcaa ccgtactgac gtgaaagaga tggtcgaact ggatcagtaa
     4321 ttcgcgatcg caaggtaaaa aaaagcaccg caattaggcg gtgctacatt aatcactatg
     4381 gacagacagg gtaaatgtac aggaagtgaa aaaaggtagc tttgctacca tggtctgaat
     4441 cgcagaccaa ttgcaaacac aacaacacaa catcacaacc gtaagccaaa agttcaccag
     4501 aacacgcatt ccgataaaac ttttcgttcc ggctcaggaa gtgccgccac tataggtatt
     4561 tgctggtaga agctcaacgg acaatttata atggctcaga ttaaaaaaac taataggtta
     4621 catagtgtga tctaattgtt aaattcattt aacatcaaag tttaatagcc atgtctaaac
     4681 gattaccacc gctaaatgcc ttacgagttt ttgatgccgc agcacgccat ttaagtttca
     4741 ctcgcgcagc agaagagctt tttgtgaccc aggccgcagt aagtcatcaa atcaagtctc
     4801 ttgaggattt tttggggcta aaactgttcc gccgccgtaa tcgttcactc ctgctgaccg
     4861 aggaagggca aagctatttc ctcgatatca aagagatatt ttcgcaatta accgaagcga
     4921 cgcgtaaact ccaggcccgt agcgccaagg gggcgttgac ggtcagttta ctccccagtt
     4981 tcgccattca ttggttggtt ccgcgacttt ccagctttaa ttcagcttat ccgggaattg
     5041 acgttcgaat ccaggcggtt gatcgtcagg aagataagct ggcggatgat gttgatgtgg
     5101 cgatatttta tggtcggggc aactggccgg ggctacgggt ggaaaaactg tacgccgaat
     5161 atttattgcc ggtgtgttcg ccgctactgc tgactggcga aaaacccttg aagacaccgg
     5221 aagatctggc taaacatacg ttattacatg atgcttcgcg ccgtgactgg cagacatata
     5281 cccgacagtt ggggttaaat catatcaacg ttcagcaagg gccaattttt agccatagcg
     5341 ccatggtgct gcaagcggct atccacgggc agggagtggc gctggcaaat aacgtgatgg
     5401 cgcaatctga aatcgaggcc ggacgtcttg tttgcccgtt taatgatgtt ctggtcagta
     5461 aaaacgcttt ttatctggtt tgtcatgaca gccaggcaga actgggtaaa atagccgcct
     5521 ttcgccaatg gatcctggcg aaagccgctg ctgaacaaga aaaattccgc tttcgttatg
     5581 aacaataatt tacgtagggt acgaccatga ccagccgttt tatgctgatt ttcgccgcca
     5641 ttagcggctt catttttgtg gctctgggcg cttttggcgc gcatgtgtta agtaaaacca
     5701 tgggagccgt tgagatgggc tggatccaga ccggcctcga ataccaggcg tttcatacgc
     5761 tggcgatctt aggtctggcg gtggcaatgc agcgtcgcat cagtatctgg ttttactgga
     5821 gtagcgtttt cctcgcgtta ggcacggtgt tgttcagcgg cagcctttat tgcctggcgc
     5881 tgtcccatct gcgtttgtgg gcgtttgtca ctccggttgg cggcgtgagc ttcctcgcgg
     5941 gctgggcgtt aatgttagtt ggtgctatcc gtttaaagcg caagggcgta agtcatgaat
     6001 aaggttgtat tgctgtgccg tccgggcttt gaaaaagagt gcgccgcaga aattaccgat
     6061 aaagccggcc agcgggaaat tttcggtttt gcccgcgtga aagagaattc gggttatgtc
     6121 atttatgaat gttatcaacc tgatgatggc gataagttaa tccgtgagct gccgttcagt
     6181 tcattaattt ttgcccgcca gtggtttgtg gtgggggaac tcctgcagca tttgccgcca
     6241 gaagatcgta ttacccccat tgtcggcatg ttacagggcg tagtagagaa gggcggtgaa
     6301 ctgcgtgttg aagttgccga taccaacgaa agcaaagagt tgctgaaatt ctgccgtaaa
     6361 tttactgttc cgctacgcgc tgccttgcgc gatgccgggg tgctggcgaa ctatgaaacg
     6421 ccgaagcgtc cggttgtgca tgtattcttt attgcaccag gctgctgcta taccggttac
     6481 tcatacagca acaataattc gccgttctat atgggcattc cgcgcctgaa atttccggca
     6541 gaggcgccga gtcgttccac gctcaaactg gaagaggcat ttcatgtgtt tattcctgcg
     6601 gatgagtggg atgaacgcct ggcgaacggg atgtgggcgg tggatttagg cgcttgccct
     6661 ggcggctgga cctaccaact ggtgaagcgc aacatgtggg tttattccgt cgacaacggc
     6721 ccgatggcgc aaagtctgat ggataccggg caggtgacgt ggctgcggga agacggtttc
     6781 aaattccgtc cgacacgcag caatatctcc tggatggtat gcgatatggt tgaaaaaccg
     6841 gctaaagttg cggcattgat ggcgcagtgg ctggttaatg gctggtgccg tgaaaccatt
     6901 ttcaatctca aactgccgat gaaaaaacgc tacgaagaag tatcacacaa tctggcgtat
     6961 attcaggcac agcttgatga acatggcata aatgctcaga ttcaggcgcg gcagttgtat
     7021 cacgatcgcg aagaagtgac ggtgcacgtc cgccgcatct gggctgcggt gggtggtcgt
     7081 cgcgacgagc gataacaggc aaggccgggg cgtcgtcttc ggcctgctac cctttccgtc
     7141 aggctgttac caaagaagtt gcaacctggc gctcatcaga aataatgtgc gttacctcat
     7201 ccagatgccc gatgcgggct tcccctgaac gattaaattt acttttatca atcaataaca
     7261 acgattgcgc ggcacgtttt aatagcatcg atttgtaatc agcgttgatc gcattggagt
     7321 cccacagtgc gccgctgcta tcgatccctt cacaagaaaa aataaacaga tcgatttcca
     7381 gcgatttaag ttgggaaatc agcgagggat tgacgtaaca gccatatttg cgctcaagtg
     7441 tgccgccgga actgatcagt tgaatgcgtt cgcgtttacc gagttcatag caaatcggat
     7501 ggctattggt gaagacctga atgttgatgt caggcaactg gcgtgccaga taccagcaag
     7561 ttgaactggc gtctaaggct atcaccatcc cttcttcaat ccacgcgagc gcctcgcgcg
     7621 cgatatctgc tttatgcgca taatggcttt tcagcctgat gtgaaaggga tcgccactgt
     7681 cttgattttg acggtggata tatttagcgc gtccatgatt gcgcagaatt ttaccctgcg
     7741 tctgtaattc attgagatcg cgacgaatgg tttctttact gacctttagc tgttcagaga
     7801 gagcttccgt ggtcaggctg gtatggttca gcagcaggtc gactatcgct tgctggcgtg
     7861 ccgctttcat atttttcacc ccatcattcg ataaccgccc gcgtcatgcg ggcgtaccgg
     7921 tatgagatta cggtgttacc ccttttttta aaagaatatt cccgtacttc gctcgttcgc
     7981 ctgtgataac gatcgcaaag gctttttgcg cccgttcata aaacgcaaaa cgattgatgc
     8041 ggatgatgtc aggacacggg gcttgtagtg aaagcgcatt acggtaacgt cgttctactt
     8101 caggatcgag agtgtcacct tctaccgccg ccatcatcac cagcggcggt gcataactgt
     8161 ccagttcaaa taacgggata atcgcctgga gcaagtcgct caccaacagg ccatcagcgc
     8221 ggatcacctg cggtcccatc gaatgggcgg gaaagtgagc atcggaaaaa ataatttcat
     8281 ctccatgtcc catctctgcc agcactttca atagttcggg agaaattaac ggcgaaattg
     8341 ttttcagcat ttcacacttc ctctataaat tcaggttcag tttgcgggta gaaataacgg
     8401 tactgataat gaatctgtgc gcgggcttct tcagggctgt taaattcccc tacgccatac
     8461 caaccgaaca gcgcagctcc tgcgacggtc gtttcggcgt cgtcgagaac ttttaccgga
     8521 atatcaagca tattggcttt aatctgattc cacaatgtgt tgcgacttcc tccaccgact
     8581 aacaataatt cagaggcctt aaagtgcccg attttttcca gcatctgtag attgcgctgt
     8641 aattgcgcag ttaacccttc cagcgccgcg cgatagaaat gcccccgcgt ggtattaagc
     8701 gtcactcctt gccagccagc gttctgacac gacaataaat cacactgcat ttttacgcca
     8761 tccgcgctag gcgcgataag acgagcttct tcaatcaaca tttgccaggg tgtttcagcc
     8821 gtccagaaca gttttctcac ccattccagc acgccggatg ccagccattg catacctggg
     8881 ttatacaacc ctgcctggct atccagttcg caggtggaac cggcgtactg acttaacagc
     8941 gaagtatcaa cctgggcgct acggactatt aaaatttccc atgtaccgga agagagcacg
     9001 ggttcatttt ggtcggcacc agcgccaaaa agggcgaact gggtatcgtg acctgcggaa
     9061 atcaccggta tgccaacggg taagccgagc attgctgcgg cgctgttctg tagcgtacca
     9121 atctgttcac ccgcttccac cagacgaggg aagagtcggc gtggaatacc agtggcttgt
     9181 aaaatttgcg gactgaaatc gcgttgctgg atatccagca tctggctggt tccggccatc
     9241 gtgatatcag tagtgaattc gccggttaaa cggtggttaa tcagcgacga aataaagaac
     9301 caggcgtgcg tgcgttccag cagttgtgga tgattttctt tcaaccacac caacttatat
     9361 aacgtattga aactaaaggc tccgacgcca gaaatagcct gcaaccgctg tgcggagatc
     9421 aactgttcaa tattgtccat aaccgctgct gttcgcggac atttccagct aatgatcgga
     9481 tagagcagat tgccttgctt atctaccaga gcgccatcca caccaaaggt ggtgacggcg
     9541 ataccgcgga tgtggcattc agtcagttca ctattgattt gccgacagca atcagcaaag
     9601 cgttgcaaaa tggcgtctaa agaccactgg tgccaggtgt tgttttccat cgcgatatcg
     9661 ctggcattag gcgttgaggc gcgggcaaca atttttccct gccgattaac cgcgatggcc
     9721 ctgacattgg tcgcgccaca gtcgagtacc aggataactt cttgtttcat aatcgctcct
     9781 gcaatacttc aatatccatg ccgtgcgcag cccaggcaga aggacgatac actttggtct
     9841 cttcaacgtt gtgcatacat accgggatac gcagcatgga ggcgagagtg ataaagtcag
     9901 cgccaacgtg gccgatggtc agaaccccat ggttagcgcc ccagttcgcc attaccgagt
     9961 acacatccgt aaacggccct ttaccggtga ggcgcggtgc aaaccaggtg gttggccagg
    10021 ttgagttggt gcgtttgttg aggatgtcat gcacatcctt cggcaattcc acgctccagc
    10081 cttccgcgat ttgcagtacc ggtcccaggc ctttgatgat gttgacacga gtcatggtga
    10141 acgggacgcc gccttcggta aggaagcggg aagagtaacc gccgccacgg aagtattcgt
    10201 ggatcgccgg gcaccattcg gtagcggcga ggcaagcgtc agcctcttgc tgagagattt
    10261 cccagtgcgg cttcatcgtc gggttacctt cgctgtcgcg ttgtttacag gaaccgtcca
    10321 gcgcagcaga acctgagttg atcaaatgga tgatgccgtg ttctgccagt ccatcgagtt
    10381 tatgccccgt tacacgctca attgcttctg gtgaccagta ggtacgcaca tcggcaaata
    10441 cctgagcggt gccggtgagc tggtgaccca ttagcattgc cacgccgtta agactgtcgt
    10501 tttcggtcgc cacgacaaag ggttcgcgca cgccattcca gtcaaatgaa ctgttgagga
    10561 tcgcttcggc ggtgtcacca ttgggatatt gatcggtcca gtgacgttgc ccctggaagc
    10621 ccgcagcgat ggcgttgtag ccaagtgatt cttccacgcg accaatatcg gccagtttgc
    10681 tgttgccttg catcatgtcg cggatacaca tcgccatcag taaactttcg cgcagaactg
    10741 cgcggctttg ctcggcattg cgttgatact gtttgttatt ttcatcttcg ccatagcgga
    10801 agtttttatc agcccaggcc agtgccattt ccagttcggc ttcgtcgtaa atcttctgat
    10861 cgatacggcg acgcagttcg gtcatatcca ccgcctggac tttcattccc agccaggatt
    10921 caaagaagtt gtgatcaacg atggaaccgg cgatacccat cgaaacgccg cccagcgaca
    10981 gatagctttt acctttcatg ctggcgacgg ccaaaccggc gcgggcaaag cgcagcagtt
    11041 tttcttcaac atcggcagga atcgatgtgt catcggcatc ctgaacgtca tgaccgtaaa
    11101 tggagaatgc cgggatgcct ttctggctgt gagctgccag agccgctgcc aggtaaacag
    11161 cgccgggacg ttcagtgccg ttaaagcccc aaatggcttt cgggcgggtt ggatccatgt
    11221 cgatggtttc actgccatag caccagcaag gcgttaccgt aatggtgagg cctacattct
    11281 ggctgctgaa tttttcttcg caagcagcgg cttcagccat acccgcgata caggtatcgg
    11341 aaatgacaca ctcgacggca gctccgcagg catggcgcag tttctcggtc agcagtgcgg
    11401 ccgtagcttt cgccatattc attgtttgtt cttcaagcga ctcacgaaca cccatgcgac
    11461 gaccgtcaat aaccgggcgg ataccaattt tcggtaagct gatttttttc attacagatt
    11521 cctcacttta ttcggaaaat atgttcagtt agttgccgtt tgagagcgga aacgggcaaa
    11581 gataaagatg accgcgaagc agagtgcggg gatcagttca gcagtgggga tgttgcccgc
    11641 cgcgtcactg acaaaaccca tgaccggagt gacaataccg ccgccaataa tggtcataac
    11701 gatgaaggac gaaccgtatt tggtgtcctg gccgagattc ttaatgccca gcgagaagat
    11761 tgttgggtac tgaatcgaca taaaggcgct gcataaagtc agggctatta agcccacatg
    11821 accgccagcg aaggctgaga tcaggcacag tgccatagcg attaatgcgt aggcggccag
    11881 gactttgtgt ggtgcgaagc gactgatgag ccaggtaccg gtgaaacgac caataaagaa
    11941 gcacaccatg gttccggtta aatagttagc ggcaaagcct gcagtcatac ctggaatttc
    12001 ttctacagcg tagcgaatca aatagctcca gcaggccgtt tgtgcgccaa cgtagcagaa
    12061 ttgcgctaat actgcccagc gccagtggcg aatacgcgcc aggcgagaaa gcgatgcgga
    12121 gaacgatcct tgtttggcgt cactgtgatt atcactctgc aatgccggga atttcgtcag
    12181 catgatcagc agggcgacca gtaacacgat agccacgatg atcatataag gtgtctgtac
    12241 cgataatacc aggctgtgtt tatacgcact caattgctct ggagacattt tatcgagaac
    12301 gtcttgcgat tgatgtggca cgttagacaa aataagactt tgcccaaaga caaccgcgat
    12361 aattgcgcca aacgagttaa atgtttgcgc aagatttaag cggaagtgac cactgctttc
    12421 cggccctaat accgtaacaa aagggtttgc ggcagtttcc agacaaccta atccggctgc
    12481 aataataaat aggccaacta aaaataaggt gtagttcatt atttctgcag cgggccagaa
    12541 taatgcagcg cccagggcat ataaaaataa cccagtaata a
//
LOCUS       DAJTSL010000120        12573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTSL010000120 DAJTSL010000000
VERSION     DAJTSL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.120"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..533)
                     /gene="rarD"
                     /locus_tag="OO433_002994"
     CDS             complement(<1..533)
                     /gene="rarD"
                     /locus_tag="OO433_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6051390.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTG"
     gene            complement(585..1058)
                     /locus_tag="OO433_002995"
     CDS             complement(585..1058)
                     /locus_tag="OO433_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6051391.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATATYMVG"
     gene            1223..2092
                     /gene="pldA"
                     /locus_tag="OO433_002996"
     CDS             1223..2092
                     /gene="pldA"
                     /locus_tag="OO433_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6051392.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2225..4054
                     /gene="recQ"
                     /locus_tag="OO433_002997"
     CDS             2225..4054
                     /gene="recQ"
                     /locus_tag="OO433_002997"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6051393.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMCKLERFGKPFMALIRAHVDGDDEE"
     gene            4118..4738
                     /gene="rhtC"
                     /locus_tag="OO433_002998"
     CDS             4118..4738
                     /gene="rhtC"
                     /locus_tag="OO433_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6051394.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(4800..5420)
                     /gene="rhtB"
                     /locus_tag="OO433_002999"
     CDS             complement(4800..5420)
                     /gene="rhtB"
                     /locus_tag="OO433_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6051395.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5531..6553
                     /gene="pldB"
                     /locus_tag="OO433_003000"
     CDS             5531..6553
                     /gene="pldB"
                     /locus_tag="OO433_003000"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6051396.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6561..7361
                     /gene="yigL"
                     /locus_tag="OO433_003001"
     CDS             6561..7361
                     /gene="yigL"
                     /locus_tag="OO433_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6051397.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7437..8336
                     /gene="bioP"
                     /locus_tag="OO433_003002"
     CDS             7437..8336
                     /gene="bioP"
                     /locus_tag="OO433_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6051398.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMNKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTLITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8224..9177)
                     /gene="metR"
                     /locus_tag="OO433_003003"
     CDS             complement(8224..9177)
                     /gene="metR"
                     /locus_tag="OO433_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6051399.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9413..11674
                     /gene="metE"
                     /locus_tag="OO433_003004"
     CDS             9413..11674
                     /gene="metE"
                     /locus_tag="OO433_003004"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6051400.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(11959..12204)
                     /locus_tag="OO433_003005"
     CDS             complement(11959..12204)
                     /locus_tag="OO433_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6051401.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12194..>12573)
                     /locus_tag="OO433_003006"
     CDS             complement(12194..>12573)
                     /locus_tag="OO433_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051402.1"
                     /translation="FNPDLAGGPIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVM
                     INRLKDIEKGKIPPEQVDLNFYTHECREYQRYCNLGWETGQPDGDAGYALWNHTHTAT
                     LEDYKLKGELNDLYHQDALDYDN"
BASE COUNT         2949 a         3169 c         3360 g         3095 t
ORIGIN      
        1 ccggtttgcg cttcaacggc aattttcttg cgtaccagtc cgtagaaggc aaaactaaac
       61 gccagcccca gcgcgatgat tggcagcgaa ccaaaagtcc acaactgaac caacacgcca
      121 catatcgcca gaatcaccgc cagccattgc atccggcgga atcgctcgcc gaggaaaatc
      181 atccccagca caatgttcac cagcgggtta ataaagtaac caaggctcgc ttccagcata
      241 tggtgattgt tcaccgccca gataaacagt agccagttgc caccaatcag cacggcagag
      301 actgccagca taaaaatttt ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg
      361 cggcaaatgc tcatcagcac caccataaag aaaaacgacc agatcacgcg atgcgtcagg
      421 atctcatcgg cgggcacgta gtaaatcagc ttgaagtatg ctggcgcaat accccaaata
      481 aaataagcgg caagagcgag taatacgccc tgccgcgttt gttttgcatc catcgggaat
      541 actcattttt aattggtaac agcagtttac ctgcttttat gtcttcaacc taccatatag
      601 gtggcggtgg cggtggcact ggcaatataa agctgttctt cattgtgtaa ttcaacgcgg
      661 gcgacggcga ctttattgcc tgcacgcaac aggctactgg tagcagtaaa acgctcgccc
      721 ctgcctgggc gcagataatc aacgcgaaga tcaatggtcc ccatccgcga tagccgctgg
      781 cgtagttcat cttcactgat ggtttcgtgg cgggttaagg tacttcccac gcacaccaga
      841 ccggcggcga catccagcgc cgacgcaatg actccgccgt gcaaaatgct ttgcgcccag
      901 ttacccacca tcatcggctg atttttaaag gccagctgtg cgaactcttt ttcgtaacgc
      961 tccagctcca tccctaacgc gcggttaaat ggcatgtgat aaacaaacat ctcacccact
     1021 aatttcaggg cttgttcagc ggtcagtacg gcagacatat catccttaca cttcattggt
     1081 taatgaaatg ttgattttat gcttctttgt tgttggtttc tactttagga agggataact
     1141 aacggcaatg gagttaagta tgtaaaatag cccgcagaaa aacattcacc ttatcaataa
     1201 ttcgttacgg agaacacgac cgatgcggac tctgcagggc tggttgttgc cggtgtttat
     1261 gttgcctatg gcagtatatg cacaagaggc aacggtgaaa gaggtgcatg acgcgccagc
     1321 ggtgcgtggc agtattatcg ccaatatgct gcaggagcat gacaatccgt tcacgctcta
     1381 tccttatgac accaactacc tcatctacac ccaaaccagc gatctgaaca aagaagcgat
     1441 tgccagttac gactgggcgg aaaatgcgcg taaggatgaa gtaaagtttc agttgagcct
     1501 ggcatttcca ctgtggcgtg ggattttagg cccgaactcg gtgttgggtg cgtcttatac
     1561 gcaaaaatcc tggtggcaac tgtccaatag cgaagagtct tcaccgtttc gtgaaaccaa
     1621 ctacgaaccg caattgttcc tcggttttgc caccgattac cgttttgcag gttggacgct
     1681 gcgcgatgtg gagatggggt ataaccacga ctctaacggg cgttccgacc cgacctcccg
     1741 cagctggaac cgcctttata ctcgcctgat ggcagaaaac ggtaactggc tggtagaagt
     1801 gaagccgtgg tatgtggtgg gtaatactga cgataacccg gatatcacca aatatatggg
     1861 ttactaccag cttaaaatcg gctatcacct cggtgatgcg gtgttgagtg cgaaaggaca
     1921 gtacaactgg aataccggtt acggcggcgc ggagttaggc ttaagttacc cgatcaccaa
     1981 acatgtgcgc ctttatactc aggtttacag cggctatggc gaatcgctca tcgactataa
     2041 cttcaaccag acccgtgtcg gtgtaggagt tatgctaaac gacttgtttt gatgaacggt
     2101 tgagtggttg gcaaatctgg aacccagcat ccaggattac cctctcagag actaaaagca
     2161 ttgcagtttc tcgcgcaggc gctgaaaata gcgcctgttt ttatttcagg caatcggggt
     2221 gaatgtggcg caggcggaag tgttgaatct ggagtccgga gctaaacagg ttttacaaga
     2281 aacctttggc taccaacagt ttcgccccgg ccaggaagaa attatcgaca ctgtgctttc
     2341 cggtcgcgat tgcctggtcg tcatgcccac cggtggcgga aaatcccttt gctatcaaat
     2401 tcctgcctta ttgctaaacg gccttaccgt ggttgtttca ccgctgattt cgttgatgaa
     2461 agatcaggtg gatcaactgc aagccaacgg cgtggcggcg gcgtgcctta actcgacgca
     2521 aacccgcgag cagcaacttg aagtgatgac aggctgccgc accgggcaaa ttcgtctgct
     2581 ttatatcgcc ccggaacgcc tgatgctgga taactttctt gagcatctgg cgcactggaa
     2641 tccggtgtta ttagccgttg atgaagcgca ctgtatctcc caatggggcc acgatttccg
     2701 cccggaatat gccgcgcttg gtcagttacg ccagcgtttc ccgaccctgc cgtttatggc
     2761 gctgactgcc acagccgacg acaccacgcg ccaggatatc gtgcgcttgc tggggctgaa
     2821 cgatccgctg attcaaatca gcagttttga ccgtccgaat attcgctaca tgctgatgga
     2881 gaagttcaaa ccgctcgatc agttgatgcg ctacgtgcag gaacagcgcg gtaagtcagg
     2941 cattatctac tgcaacagcc gcgcgaaagt agaagacacc gctgcgcgcc tgcaaagcaa
     3001 gggaattagc gcggcggcct atcatgctgg gctggaaaat aatgttcgcg ccgacgtgca
     3061 ggaaaaattt cagcgcgatg acctgcaaat tgtggtggcg acggtggcgt tcggcatggg
     3121 catcaataaa ccaaacgttc gcttcgtggt ccactttgat attccgcgca atatcgaatc
     3181 ctattatcag gaaaccggtc gcgccgggcg tgatggcctg cccgcggaag cgatgctgtt
     3241 ttacgatccg gccgatatgg cgtggctgcg ccgttgcctg gaagagaagc cgcaggggca
     3301 gttacaggat atcgaacgtc ataaactcaa tgcgatgggc gcttttgccg aagcgcaaac
     3361 ttgccgtcgt ctggtattgc tgaactattt tggcgaaggg cggcaagagc cgtgcgggaa
     3421 ctgcgatatc tgcctcgatc cgccgaaaca gtacgacggt tcaaccgatg ctcagattgc
     3481 cctttccacc attggtcgtg tgaatcagcg gtttgggatg ggttatgtgg tggaagtgat
     3541 tcgtggtgct aataaccagc gtatccgcga ctatggtcat gacaaactga aagtctatgg
     3601 catgggccgt gataaaagcc atgaacattg ggtgagcgtg atccgccagc tgattcacct
     3661 cggcctggtg acgcaaaata ttgcccagca ttctgcccta caactgacag aggccgcgcg
     3721 cccggtgctg cgcggcgaat cctctttgca acttgccgtg ccgcgtatcg tggcgctcaa
     3781 accgaaagcg atgcagaaat cgttcggcgg caactatgat cgcaaactgt tcgccaaatt
     3841 acgcaaactg cgtaaatcga ttgccgatga aagtaatgtc ccgccgtacg tggtgtttaa
     3901 cgacgcaacc ttgattgaga tggctgaaca gatgccgatc accgccagcg aaatgctcag
     3961 cgttaacggc gttgggatgt gcaagctgga acgctttggt aaaccgttta tggcgctgat
     4021 tcgtgcgcat gtcgatggcg acgacgaaga gtagtcagca gcataaaaaa gtgccaggat
     4081 gaagactccg taaacgtttc ccccgcgagt caaatgtatg ttgatgttat ttctcaccgt
     4141 cgccatggtg cacattgtgg cgcttatgag ccccggtccc gatttctttt ttgtctctca
     4201 gaccgctgtc agtcgttccc gtaaagaagc gatgatgggc gtgctgggta ttacctgcgg
     4261 cgttatggtt tgggcaggga ttgcgttgct tggcctgcat ttgattatcg aaaaaatggc
     4321 ctggctgcat acgctgatta tggttggcgg tggcctgtat ctctgctgga tgggttacca
     4381 gatgctacgc ggtgcactga aaaaagaggc tgtatctgcg cctgcaccac agattgagct
     4441 ggcgaaaagc gggcgcagtt tcctgaaagg tttactgacc aatctcgcta atccgaaagc
     4501 gattatctac tttggctcgg tgttctcatt gtttgtcggt gataacgttg gcactaccgc
     4561 gcgctggggc atttttgcgc tgatcattgt cgaaacactg gcgtggttta ccgtcgttgc
     4621 cagcctgttt gccctgccgc aaatgcgccg tggttatcaa cgtctggcga agtggattga
     4681 tggttttgcc ggggcgttat ttgccggatt tggcattcat ttgattattt cgcggtgatg
     4741 ccagacgcgt cttcagagta agtcggataa ggcgtttacg ccgcatccga cattattttt
     4801 cacgcatgcc tcgccgatgc taacagcgct cccaccagca taaacaacga gccgaaaatc
     4861 ttattcagcg ccttcatctg ctttggtcct ttaatccata gagcaatccg ttgagcaagg
     4921 gtggcgtaac cgatcatcac aataatatcg actacaatag tggtgacgcc gagcacgata
     4981 tactgcatca gttgcggctg ctgtggcatg atgaattgcg gaaatagcgc cgccagaaac
     5041 acaatacttt tgggattggt gagattcaca aaaactgcgc gctggaacaa atgtcgacgc
     5101 gattgagtag aggcaagcga tttaaggtca attgcaccag cggcgcgcca ctgctggatt
     5161 cccagccaaa tcaagtaagc cgcgcctgcc cacttcaaca cttcaaacgc aatcactgag
     5221 cgggaaaata gcgtccccaa acccacgcca accagcacaa tatgaatcgc cagtccggtc
     5281 tgaagcccag caatagacgc caccgcgccg cgataaccgt ggttgagcga ggtggtcata
     5341 gtgttgattg caccagagcc tggcgacagg cttaaaatga tcgatgtcag caggtaggca
     5401 aaccaccatt ctaaggtcat gatgaactcc cggtgtgtct atttttgtgc cacaatacgc
     5461 tactgtcgca gcgttgtgtc aggcacgcta aaaaaaacga ttttacgtgg tttaagaggc
     5521 agattacccg atgtttcagc agcaaaaaga ctgggaaaca agagaaaacg cgtttgctgc
     5581 ttttaccatg ggaccgctga ctgatttctg gcgtcagcgt gatgaagcag agtttactgg
     5641 tgtggatgac attccggtgc gctttgtccg ttttcgcgca cagcaccatg accgggtggt
     5701 agtcatctgc ccggggcgta ttgagagcta cgtaaaatat gcggaactgg cctatgacct
     5761 gttccatttg gggtttgatg tcttaatcat cgaccatcgc gggcagggac gttccggtcg
     5821 cctgttagcc gatccgcatc tcgggcatgt taatcgcttt aatgattatg ttgatgatct
     5881 ggcggcattc tggcagcagg aggtccagcc cggtccgtgg cgtaaacgct atatactggc
     5941 acattcgatg ggcggtgcga tctccacatt atttctgcaa cgccatccag gtgtatgtga
     6001 cgccattgcg ctaactgcgc caatgtttgg gatcgtgatt cgtatgccgt catttatggc
     6061 acggcagatc ctcaactggg ccgaagcgca tccacgtttc cgtgatggct atgcaatagg
     6121 caccgggcgc tggcgcgcgt tgccgtttgc tatcaacgta ctgacccaca gcagacagcg
     6181 atatcgacgt aacttacgct tctatgctga tgacccaacg attcgcgtcg gtgggccgac
     6241 ctaccattgg gtacgcgaaa gtattctggc tggcgaacag gtgttagccg gtgcgggtga
     6301 tgacgccacg ccaacgcttc tcttgcaggc tgaagaggaa cgcgtggtgg ataaccgcat
     6361 gcatgaccgt ttttgtgaac tccgcaccgc cgcgggccat cctgtcgaag gaggacggcc
     6421 gttggtaatt aaaggtgctt accatgagat cctttttgaa aaggacgcaa tgcgctcagt
     6481 cgcgctccac gccatcgttg attttttcaa caggcataac tcacccagcg gaaaccgctc
     6541 tacagaggtt taaatttctt atgtaccagg ttgttgcgtc tgatttagat ggcacgttac
     6601 tttctcccga ccatacgtta tccccttacg ccaaagaaac tctgaagctg ctcaccgcgc
     6661 gcggcatcaa ctttgtgttt gcgaccggtc gtcaccacgt tgatgtgggg caaattcgcg
     6721 ataatctgga gattaagtct tacatgatta cctccaatgg tgcgcgcgtt cacgatctgg
     6781 atggtaatct gatttttgct cataacctgg atcgcgacat tgccagcgat ctgtttggcg
     6841 tagtcaacga caatccggac atcattacta acgtttatcg cgacgacgaa tggtttatga
     6901 atcgccatcg cccggaagag atgcgctttt ttaaagaagc ggtgttcaaa tatgcgctgt
     6961 atgagcctgg attactggag ccggaaggcg tcagcaaagt gttcttcacc tgcgattccc
     7021 atgaacaact gctgccgctg gagcaggcga ttaacgctcg ttggggcgat cgcgtcaacg
     7081 tcagtttctc taccttaacc tgtctggaag tgatggcggg cggcgtttca aaaggccatg
     7141 cgctggaagc ggtggcgaag aaactgggct acagcctgaa ggattgtatt gcgtttggtg
     7201 acgggatgaa cgacgccgaa atgctgtcga tggcggggaa aggctgcatt atgggcagtg
     7261 cgcaccagcg tctgaaagac cttcatcccg agctggaagt gattggtact aatgccgacg
     7321 acgcggtgcc gcattatctg cgtaaactct atttatcgta atcgttcttt atttggtcag
     7381 ttgtcaacct gatacttcgc tacaatggat actcgttaat caaagagttt tccattgtgg
     7441 cgctacttat catcaccacg attctgtggg ccttctcctt tagcttttat ggcgagtacc
     7501 ttgcggggca cgtcgatagc tattttgcgg tgctggtgcg cgttggcctg gcggcactcg
     7561 tttttctgcc gtttctgcgt acccgtggca atagcctgaa aacagtcggc ctgtatatgc
     7621 tggtgggcgc gatgcagctc ggcgtgatgt atatgctgag tttccgcgct tatctctatc
     7681 tgacggtttc cgaactgctg ctgttcaccg tactgacgcc gctctacatc acgctgattt
     7741 atgacatcat gaataagcgc cgtctgcgct ggggatatgc ttttagtgcc ttgttggcgg
     7801 tgattggtgc cgggattatt cgctatgatc aggtcaccga ccatttctgg actggcttgt
     7861 tgctggtgca actctccaat atcacttttg ccatcggcat ggtgggttac aaacgcctga
     7921 tggaaactcg cccgatgccg cagcataacg cctttgcgtg gttctatctt ggcgcgttcc
     7981 tggtggcggt aattgcatgg ttcttgctgg gaaatgcgca gaaaatgccg caaaccacgt
     8041 tgcaatgggg cattctggtg tttcttggcg tggtggcttc cgggattggc tactttatgt
     8101 ggaactacgg cgcgacgcag gtggacgccg gaacgctggg aattatgaat aatatgcacg
     8161 ttccggcagg gctgctggta aacctggcta tctggcacca acagccgcac tggccaacgt
     8221 tgattacagg cgcgctggtg atcctggcct cactgtgggt gcatcgtaag tgggtcgctc
     8281 cgcgctcttc acaaacggca gatgatcgca ggcgtgattg cgcgctgagc gaataaacgc
     8341 ttccgtaact ggctgacgct gctcgccatc gcgcacggcg gcgtacagtc ggctccacaa
     8401 gccttcgccc agggttttgg tcaccaccag accctggcgc tcaaaactct ctactaccca
     8461 atgcggtagc gcggcaatac ccatccgcgc ggcaaccatc tgaatcaaca ataaggtgtt
     8521 atcgacgctt ttcagtgacg gactgacgcc tgccggctga agaaaatgcc gccagacatc
     8581 cagtcgacta cgctgcaccg gataaattaa tagcgtctcg ctggcgagat cttccggtgt
     8641 aatacgcgtt tttgccgcca gtggatggtc aggtgctaac accagacgca cttcatagtc
     8701 gaacatcggc gaataatgca gaccactgcg cggcagaata tcggacgtca ttaccagatc
     8761 cagctctccc tgttgcaagg cgggctgcgg gtcaaatgtc acgcccgatt taaagtccat
     8821 ctcgacctgc ggccagttct tatggaaatt ttctaacgcg ggcgtcagcc actgtataca
     8881 gctatggcac tcaatggcaa tgcgcagacg cgtctgctgc ggttcattgc aggcttgcag
     8941 tgcctggcta atttgtggca gcacctggtt tgccagttgc aacaggattt ctccctgcgg
     9001 tgtaaagcga agcggctggc ttttacgcac aaatagccgg aagccaaggc gttgttccag
     9061 atcgctaaac tggtgagaca gggcggattg cgtttgatgc aacgtcgccg cagcggctgc
     9121 gagtgagccg cagttccgca acgcttgtag cgttttcaga tgttttactt cgatcatgaa
     9181 agtccttcac ttcggcatga ataatttgcg cttgaggaat atacagtaac cgccaattat
     9241 ggatgtgtaa acatctggac ggctaaaatc cttcgtcttt taaatttatg gtgcgttggc
     9301 tgcgtttctc caccccggtc acttacttca gtaagctccc ggggatgaat aaacttgccg
     9361 ccttccctaa attcaaaatc cataggattt acatataatt agaggaagaa aaatgacaat
     9421 attgaatcac accctcggtt tccctcgcgt tggcctgcgt cgcgagctga aaaaagcgca
     9481 agagagttat tgggcaggga actccacgcg tgaagaactg ctggcggtag ggcgtgaatt
     9541 gcgtgctcgt cactgggatc aacaaaagca agcgggtatc gacctgctgc cggtgggcga
     9601 ttttgcctgg tacgatcatg tactgaccac cagtctgctg ctgggtaacg ttccggcgcg
     9661 tcatcagaac aaagatggtt cggtagatat tgacaccctg ttccgtattg gtcgtggacg
     9721 tgcgccgact ggcgaacctg cggcggcagc ggaaatgacc aaatggttta acaccaacta
     9781 tcactacatg gtgccggaat tcgttaaagg ccaacagttc aaactgacct ggacgcagct
     9841 gctggaggaa gtggacgagg cgctggcgct gggccacaac gttaagcctg tgctgctggg
     9901 gccggttacc tggctgtggc tggggaaagt gaaaggtgaa cagtttgacc gcctgagcct
     9961 gctgaacgac attctgccgg tttatcagca agtgctggca gaactggaga aacgcggcat
    10021 cgagtgggta cagattgatg aacccgcgct ggtactggaa ctaccacagg cgtggctgga
    10081 cgcatacaaa cccgcttacg acgcgctcca gggacaggtg aaactgctgc tgactaccta
    10141 ttttgaaggc gttacgccaa atctcgacac gattactgcg ctgcctgttc agggactgca
    10201 tgtggacctt gtacatggta aagatggcgt tgctgaactg cacaagcgcc tgccttctga
    10261 ctggctgctg tctgcgggac tgatcaatgg tcgtaacgtc tggcgcgccg atcttactga
    10321 gaaatatgcg caaattaagg acattgtcgg caaacgtgat ttgtgggtgg catcttcctg
    10381 ctcgttgctg cacagcccca tcgacctgag cgtggaaacg cgtcttgatg cagaagtgaa
    10441 aagctggttt gccttcgcct tacaaaaatg ccatgaactg gcactgctgc gcgatgcgct
    10501 gaacagtggt gacacggcag ctctggcaga gtggagcgcc ccaattcagg cgcgtcgtca
    10561 ctctactcgt gtacataatc cggcggtaga aaagcgtctg gcggcgatca ccgcccagga
    10621 cagccagcgt gcgaatgtct atgaagtgcg tgctgaagcc cagcgtgcgc gttttaaact
    10681 gccagcgtgg ccgaccacca cgattggttc cttcccgcaa accacggaaa ttcgtaccct
    10741 gcgtctggat ttcaaaaagg gcaatctcga cgccaacaac taccgcacgg gcattgcgga
    10801 acatatcaag caggccattg ttgagcagga acgtttggga ctggatgtgc tggtacatgg
    10861 cgaggccgag cgtaatgaca tggtggaata ctttggcgag cacctcgacg gatttgtctt
    10921 tacgcaaaac ggttgggtac agagctacgg ttcccgctgc gtgaagccac cgattgttat
    10981 tggtgacgtt agtcgcccgg caccgattac cgtggagtgg gcgaagtatg cgcaatctct
    11041 gactgataaa ccggtgaaag ggatgctgac tggcccggtg accattctct gctggtcgtt
    11101 cccgcgtgaa gatgtcagcc gtgaaaccat cgccaaacaa attgcgctgg cgctgcgtga
    11161 tgaagtggcg gacctggaag ccgcaggaat tggcatcatc cagattgatg aaccggcgct
    11221 gcgcgaaggt ttaccgctgc gtcgtagcga ctgggatgca tatctccagt ggggcgtgga
    11281 ggccttccgt atcaacgccg ccgtggcgaa agatgacaca caaatccaca ctcacatgtg
    11341 ttattgcgag ttcaacgaca tcatggattc cattgcggca ctggatgcag acgtcatcac
    11401 catcgaaacc tcgcgttccg acatggagtt gctggagtcg tttgaagagt ttgattatcc
    11461 gaatgaaatc ggtccaggcg tctatgacat tcactcgcca aacgtaccga gcgtagaatg
    11521 gattgaagcc ttgctgaaga aagcggcaaa acgcattccg gcagagcgcc tgtgggtcaa
    11581 cccggactgt ggcctgaaaa cgcgcggctg gccagaaacc cgcgcggcac tggcgaacat
    11641 ggtgcaggcg gcgcagaatt tgcgtcgggg atgatgaata ttaacgagtc actgtaaatt
    11701 gcagtacgat gaaagaggca aaactgcctg ataccaggcc tacataattt atgcaattta
    11761 ttgaattttt gagttttgtt ggccggacaa ggcgttcacg ccgcatccgg catgaacaac
    11821 gcgcactttg ccaataatct gatggcttcc atcaccggaa gccattttgc tatctgcgcc
    11881 cgttcagata cagaaataac cgaatcattt ttacgcaaaa tataatcctg agcgatttgt
    11941 tcagtaatca acgctacgtt atttattctc acttttcacg aaattgtatc ccttttcagt
    12001 aacgtggtag tgaggcatag acttatgtcc gctatcctca ataataacca aaccctcgtt
    12061 agctaatttg ttaacaatgt taacaacatt accgaatcct ggaatttgcc ttatggatag
    12121 tgtgcgatcg agattcatcc agttccagtt tatatctttc tcagccataa ctttcagtac
    12181 gttaatttca gtttcagtta tcatagtcaa gtgcatcctg gtggtataag tcattaagtt
    12241 ctcccttaag tttataatct tctagagtag cagtatgcgt atgattccag agtgcatagc
    12301 ctgcgtcacc atcaggttgt cctgtttccc aaccaagatt acagtaacgc tgatattccc
    12361 gacactcatg agtatagaag ttaagatcta cttgttctgg ggggattttt cctttctcaa
    12421 tgtctttcag tctattgatc atcacttcat ttacagggtc atgaccaaat ctggctatgt
    12481 gtttttcaac aatagcgata ccttccctgt tgattgttac gccatctgtc gtcagatttt
    12541 ctattggacc accggcaagg tcgggattaa atg
//
LOCUS       DAJTSL010000121        12468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTSL010000121 DAJTSL010000000
VERSION     DAJTSL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.121"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..204)
                     /gene="chbB"
                     /locus_tag="OO433_003007"
     CDS             complement(<1..204)
                     /gene="chbB"
                     /locus_tag="OO433_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6051403.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQ"
     gene            complement(502..840)
                     /gene="osmE"
                     /locus_tag="OO433_003008"
     CDS             complement(502..840)
                     /gene="osmE"
                     /locus_tag="OO433_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6051404.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1042..1869
                     /gene="nadE"
                     /locus_tag="OO433_003009"
     CDS             1042..1869
                     /gene="nadE"
                     /locus_tag="OO433_003009"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6051405.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2099..2986
                     /gene="cho"
                     /locus_tag="OO433_003010"
     CDS             2099..2986
                     /gene="cho"
                     /locus_tag="OO433_003010"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6051406.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(2946..3521)
                     /gene="ves"
                     /locus_tag="OO433_003011"
     CDS             complement(2946..3521)
                     /gene="ves"
                     /locus_tag="OO433_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6051407.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3724..4194)
                     /gene="spy"
                     /locus_tag="OO433_003012"
     CDS             complement(3724..4194)
                     /gene="spy"
                     /locus_tag="OO433_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6051408.1"
                     /translation="MRKLTALFVASTLALGAANLAHAAAPADAKPMMHHKGKFGPHQD
                     MMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIVSDTFDKAKAEAQIAK
                     MEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE"
     gene            complement(4524..5492)
                     /gene="astE"
                     /locus_tag="OO433_003013"
     CDS             complement(4524..5492)
                     /gene="astE"
                     /locus_tag="OO433_003013"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6051409.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5485..6828)
                     /gene="astB"
                     /locus_tag="OO433_003014"
     CDS             complement(5485..6828)
                     /gene="astB"
                     /locus_tag="OO433_003014"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR6051410.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6825..8303)
                     /gene="astD"
                     /locus_tag="OO433_003015"
     CDS             complement(6825..8303)
                     /gene="astD"
                     /locus_tag="OO433_003015"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR6051411.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKLRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGRANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQLETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8300..9334)
                     /gene="astA"
                     /locus_tag="OO433_003016"
     CDS             complement(8300..9334)
                     /gene="astA"
                     /locus_tag="OO433_003016"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6051412.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWHNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAVEKTA"
     gene            complement(9331..10551)
                     /gene="astC"
                     /locus_tag="OO433_003017"
     CDS             complement(9331..10551)
                     /gene="astC"
                     /locus_tag="OO433_003017"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR6051413.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIMAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGDFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            10997..11803
                     /gene="xthA"
                     /locus_tag="OO433_003018"
     CDS             10997..11803
                     /gene="xthA"
                     /locus_tag="OO433_003018"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6051414.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            11970..>12468
                     /locus_tag="OO433_003019"
     CDS             11970..>12468
                     /locus_tag="OO433_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6051415.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISA"
BASE COUNT         3026 a         3377 c         3059 g         3006 t
ORIGIN      
        1 ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg
       61 acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt
      121 ttctgcctgt gcgcgcattt ttgataccag taaagaggta gacatgcccg cagaacaaaa
      181 cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga
      241 cactccgcaa gccttaacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt
      301 tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta
      361 taaattgtct ctccttgacc tgcggttatg accctcttca catttcgggc aaataattcg
      421 cgaagataat taagaactca ggccaaaaaa accggcgcaa tggccggttt ccgttgttac
      481 tcaagctttc aaacgaagtg attacttcgc agcctgtgga tcagtgtcgt attcagcaca
      541 ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc
      601 tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccacgag catggatcat
      661 gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt
      721 tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat aagccgtaca
      781 acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat
      841 attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc
      901 aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt ttatccacca
      961 ttcaacccgc tgtcgcttgt cgtttcagta gcgacgggtt agctttaagg aagttttgtc
     1021 ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca
     1081 aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg tcgattttct gaaaagctac
     1141 ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc
     1201 acgcttgccg gtaagctgtg ccagatggcg attaatgagc tgcgccagga gaccggcaac
     1261 gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggccga cgaacaagat
     1321 tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc
     1381 gcggtattgg ccagcgaaca ggcgctgcgc gaagcgggca ttgaactgag cgattttgtc
     1441 cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc
     1501 agcggtgtcg tggtgggcac cgatcatgca gcagaagcta ttaccggatt cttcactaaa
     1561 tatggtgacg gcggtacgga cattaatccg ctgtatcgtc tcaacaaacg tcagggtaaa
     1621 cagttactgg cggcattagc ttgcccggaa cacctttata agaaagcgcc aacggccgat
     1681 ctggaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat
     1741 atcgacgact atctggaagg gaaaaacgta cctcaacagg tcgccagaac aatagagaac
     1801 tggtatctga aaaccgaaca taaacgccgt ccgccaatta ccgttttcga tgatttctgg
     1861 aaaaagtaat aatttgcaca tattggattg tgcgaaaaag agtaatttgt tcacgccgga
     1921 tgcggcgtga acgccttatt cgacctataa aactatgcaa attcaataca ttgcaggagt
     1981 cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc gacccgcctc
     2041 ttttttaatc gcttcccgcc tgttacactg gatagataac cagcattcgg agtcaacagt
     2101 ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc
     2161 cgaacattta cgttcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca
     2221 tggtgaaagt gacaccatgc cgctctatat cggcaaaagc gttaacatcc gcagccgcgt
     2281 cctttctcat ttacgtaccc cggatgaagc cgccatgcta cggcaatccc gacggatcag
     2341 ctggatatgt accgccggtg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga
     2401 acaacagccg ctgtttaata aacggttgcg ccgcaatcgt cagctctgtg ccctgcaatt
     2461 aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa
     2521 cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga ccatcgctga
     2581 tgaacaaaaa ctttgttatg gcctgctggg actggaaccg ttaagtcgcg gtcgtgcatg
     2641 ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga gccatgagga
     2701 acatgcgcta cgcttgcgcc agtctctgga gcgtttgcgg gtggtgtgtt ggccttggca
     2761 aggggcggtg gcgctgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa
     2821 ctggctgtgg ctgggggcgg ttaattcgct ggaagacgcg acaacgttaa ttcggacacc
     2881 cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta
     2941 tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac
     3001 agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca gcacgcgccc
     3061 tggtcggtag tgagcaattt atcgcccagt tgccacgccc cgttaatcac aaacaccacc
     3121 ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag
     3181 acatccaggc gtgtcatgat gttgaaatcc atcgacatct gcccttccgt cagtttcgct
     3241 tttaccacct ggtccgctgc aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag
     3301 cggtctgcgc tttcaaggag catctcgccg ccttccagca acgtcactat cctttccatg
     3361 ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa
     3421 aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcatttcgc
     3481 cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc
     3541 ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt
     3601 tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac cgccgatcct
     3661 gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa
     3721 agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagacgctt
     3781 ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aggatgttgt aaattttgtt
     3841 ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt ttgcgatctg
     3901 cgcttcagct tttgctttat cgaaggtatc gctgacaatg atgtcatgca ttgcgcggcg
     3961 ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt cgcggatctg
     4021 ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat gcggaccgaa
     4081 cttgcctttg tggtgcatca tcggcttcgc gtcagccggt gctgcggcat gggccaggtt
     4141 agccgcgcca agagccaggg tagaggcaac aaacagtgca gttaatttac gcatattcta
     4201 tatccttcct ttcagttatt tattacggct ttcttaagta gcgtgccgtg ttgacaagat
     4261 taactttact gactttagcg tcaattaatc agagcaacgg taaaacaatg aaagtgtaaa
     4321 aaacactttt gcgccaatta tggagaaaaa aagaaaattt gatggagagt gatgagagaa
     4381 tattacaaca caatgatttt gcagagatta tgaagaacta tactggatga ctggtgataa
     4441 ataaagcaaa taaccaggat taatctgtat taatttataa gaaagcaact taatacccgc
     4501 agaatgattt ctgcgggtaa gtattagctt attttttcga gcattaatcc cgcgcgtaat
     4561 cccaacgcta ccaacggatt agggaataac acatactcta catcatgggt tacggtaaaa
     4621 cgttcctctc cgtcctgcgc cagcaatgtt cctttctcaa acggcataaa attcagcgtg
     4681 tcacttgcca tatgcatttc gaaggacggc gagtgacgag taatttgcga aaccacccga
     4741 taacggagcg gcggtgttct cacgataccg acactctcac cagatagcag cgcagcaatt
     4801 gcgctggcag ttactgcaaa ctggcgaaga tcgttttgcc caaagggcaa cgctttgcca
     4861 agttccagcg tacaggccag cgcgccaaaa tgtctggcgc tgaaatgggt aaacgtacca
     4921 ccaggttcct gatggaacac cagcgcctcc agccccgccg cacccagcca cgtcagaaat
     4981 ttctcgtccc agggaatgtc gcgttgcggt aatacaccga actgcggatg caaggagcca
     5041 cgaattgcgg tatgtagatc aaggtgccag cgcacagatt ctttgccctg gtcataaaaa
     5101 tcttccaggc actgttccag ttcgcgcgcc cgacaggttt ctccgctttc agcaaatagc
     5161 tgccaacgac cgccaaacat tcgattcata tcgctatggc aataacgttt cccttgcttc
     5221 agcgcaggag gattcccgag gatcaccagc aaccgccaac gtaacgggat ctcgccgtga
     5281 gatatcgcgc caagcaacgc gtccagcatt tccacaggtg ccgtctcatt accgtggatc
     5341 cccgcagaaa tcaccagcgc gccttgcggt ggcgttaatg gcgtcagttc cagcacacca
     5401 tcgcccagcc agcgccagcg aacgccgttg atttctcgct cggtgataac cggtttttta
     5461 cccgttaagg tcagagcaag aaaattatcc attgccccct ccctcgcgct ggaacggata
     5521 aaccgaaccg agattcagta attggctcaa tacatccagt gcttcccgcc cttcgcgcag
     5581 caattgcggg tcggccagat cggcagcagt aagacgatcg cggtagtaac gatccaccca
     5641 gtcattgagc gcattaaaca aggtatcgtt catcatcacc gccggattca ccgcccggcg
     5701 ttcttcttct gtcaatacca cccgcaaccg caggcacgcc gggccgccgc cattcgccat
     5761 gctttcacgt aagtcaaaga cttttagttc gctaatcggg ttgtcagcgg caaggagttc
     5821 attgagataa ccccatactc cggcgtgttc ccgacactcc tgaggcagca ccaacatcat
     5881 ggaaccatca tcgcggctca acagttggct gttaaacaga taggtagaca ccgcatccga
     5941 cacggaaacc tgagttgccg gaacttctat cgccataaaa ccgttcaccc gcgcccgcag
     6001 gtttgccagt aactgtgact ggcgagcgaa cgcctgttgg tggcaaaaca gcacctggcg
     6061 gttacttacg gcaatcacgt cattatgaaa aacaccctgg tcgataacgt ccgggttttg
     6121 ctgggcgaaa atcacctgtt ggggattcac ctgattaagc cttgccaccg cctcgctggc
     6181 ttcgcgagtc tgtcgcgccg gataacggga aggccgggta tcattgcctt cttctcgccc
     6241 gtagacaaaa agttgcatac ccggctcacc gtaatgaccg ccgagacgat tgtggtttgc
     6301 cgccccctca tcaccgagca acgctacctg cggcaacgcc gaatggacgc taaatttctc
     6361 ttcgtcgtta aaaatagctt ttaacagcga ttcagtgacg ggcgcttcca gcgaacggtg
     6421 aaatttattg ttcaggttgg caacggtgag atgcactttg ccatccagcg tatcggcaga
     6481 tggcgcgatc gttgccgcat tggctaccca cattggcgaa gcggagctga cgctggaaag
     6541 ccagtgcggt gcctggcgtg caactttttc cagtacctgc tcatcgctac cactgaatcc
     6601 caactgacgc agcaccggaa taaacggacg ctcgtgcggc gggatcacgg cctgggggaa
     6661 tcctgcatcg gcaagggttt tcattttcag taagccctgt ttcgccgcca gtcgcgggtt
     6721 agacacctga aaacggtgac gggtagaggc ttcattacca aacgacaggc cagcgtaatg
     6781 atgcgtcagc cctaccagcc cgtcgaaatt aacttcccag gcgttcatcg caccacctca
     6841 tcggaaaaat ccagcccggg gttaagcgtt gcgggcaaag ttaacgagtc cgactccagg
     6901 ctcgccatag gccatgcgca gtaatctgcg gcataccagg cgctggggcg atggttaccg
     6961 gatgcaccaa tgccgccgaa tggcgcggta ctggcagcac cagtaagcgg tttgttccag
     7021 ttaacaatcc ccgcccgcgc ctccagcaac agttgatcga atttttcccg ttcgggggaa
     7081 accagaccgc aagagagtcc gaagcgagtg ttattcgcca ttagaatcgc ttcctcgaaa
     7141 gaatcataac gccagacgcg caataacggt ccgaacacct cttcatctgg tacgccagca
     7201 acgcctgtca tttcaatgat ccccggcgtc agcaacgatg tctctaattg taataagcgc
     7261 ggcgcaagca gggttcgtcc gcccatcgct tccagttgct gccaggcagt aaccacctgc
     7321 tgtgcggcct gttcagaaat cagcccgcca ataaacggct gcggttcgtc atcccagttg
     7381 cccggcgtta atcgctggct gacggcaacc agacgagcaa gaaacgcatc gccctgcgct
     7441 ccgcttttga gcaataaacg gcgggcgcag gtgcagcgtt gcccggctgt gacaaacgcc
     7501 gactgaatgg tcagatggac agccgcgtcg atatccgcca cctcatcgat aattagcgga
     7561 ttattaccgc ccatctcaag ggcgagaatt ttctccggct gaccggagag ctggcgatgc
     7621 agctggtagc cggtattggc gcgaccggta aacagcaaac cgtcgagatc ctccagcgca
     7681 ctcagcgcct gacccgtttc acgcccgccc tgcaccaggt tcagcacgcc cggcggcaag
     7741 ccagcctgct gccataaacg cattaccgct tcgccactcc acggtgtcag ttcgctgggt
     7801 ttaaagataa tggtgttacc tgccagcaat gccggaacga tatgtccgtt cggcaaatga
     7861 ccagggaaat tatacggccc aaacaccgcc agcacgccgt gcgggcgatg tcgcaggctc
     7921 gccgcaccgt ccggcatttc actacgctgc tcgccggtac gaacgtgata cgccttaatt
     7981 gatatcgcga ttttattgat catcgccgtc acttcggttg ccgcttccca gcgcagctta
     8041 cccgtttctc tggcaataat cgcggttaat tcggctttat tgctttccag caatccggca
     8101 aagcgttcga caacgacctg acgttcagcc aatgagagcc gcgcccagcg cggaaacgcc
     8161 gcacgggctg cccgacaagc ctgttcgacc tgagcggcat cggcatcatt gccttgccat
     8221 aacacctcgc ccgataccgg attacgcttc acacgcgatg cgccctggcc cgttatccag
     8281 tcaccgttaa tccataaagt catgctgttt tctccactgc gcacaggcgc accagacgaa
     8341 cgcgatcccc tgcgtggcat ttgagggcat ccagttgtgc ggcggttaaa atcaaacgct
     8401 cggttgccgg atcggtacgc gccagcacca cgcggaaatg gtgataattt tcattggcga
     8461 ccaggcaggc cggaaaatcg ccctgcgcag gctgcccttc tgccacttcc accagccgac
     8521 ttttacggat ggcgcgcacg cggtcgatgt cacactcaag cgttggcccg ccgtcgaaga
     8581 tgtcgatata gttgcggtaa cgaaaacctt ctttctccag caccgcgcgg gcaggcgcgg
     8641 tttgcggatg tacctgaccg atgacgtcct gggcttcctg ggataaaaag tgggtataga
     8701 tcggatgttt cggcatcagt tctgcaataa atgccttttg cccggtgccg cagagaaaat
     8761 cggcgcggct aaaatccatc gaaaagaagc gtttaccgag gctttgccag aacggtgaat
     8821 agccgtgttc gtcaatcacc ccgcgcattt cagcaaccac tttgtcatta aacttgtcgc
     8881 gaaaagccgc cataaacata aagcgcgatt tcgacagcaa atagccgttg ccctctttgc
     8941 gccagtccgg gtcgagaaac agcgtgcaca gctcgctgct gccggtgtga tcgttactaa
     9001 gaaacagcgt cggcaatgcg ttatagacat tcagctcttt tgaggcgtga accaacgtgc
     9061 cgacccgata gttgtgccag ggatcgttca gcccaaccgc cacctcaatg gcgcaaatcc
     9121 ccgccacggt gcctgtctcg ctatcttcca gcacgaacac atagccctgc tcgcttttgg
     9181 gcagttcgcc ttgccaggtt ttgattgccc tttcgatacg cgcggaaagc gtggcttcat
     9241 tagcgggaag cgacgtcagg ccgccgcccg ttttgctggc aagctgcatc agcgccgaga
     9301 catctgagcg ctcaacggga cggatgacca tcatgatgaa cctcggctaa caaaatgttc
     9361 gcaagcagct gcaaagcgat ccagtccggt cgtcacctct tcttcgctga cattgagcgc
     9421 aggcgcaaaa cgcaccacgt tgccacccgc aatcagtacc ataacgcctg ctttcgccgc
     9481 ctcctgagag atctgtttcg cttgtccggc gtaatcggca ttcagtacac agccaatcaa
     9541 caaacctaag ccgcgaactt cactgaacaa accgtagcgg tgattaatgg tattaagacg
     9601 ctcaacaaac cagtcgtgac gctgtttaac gccattaagc atctctggtg tgttgatgag
     9661 ctccagcact ttgcccgcca ccgccgaggc cagcggattg ccgccatagg tggtgccatg
     9721 agtgccaacg gtcatcacgc tggcgcactc ttcggttgtt aacaacgcac cgaccgggaa
     9781 atcgccgccc agcgctttgg cggtagttaa cagatcaggt gtcacgccgt agtgcatata
     9841 ggcatacagt tccccggtgc gcccgacgcc ggtttgtact tcatcaaaaa tcaacagcgc
     9901 attgtggcgg tcacacaatt cacgcagacc ttgtaaaaac gcgttgctgg ctggcaccac
     9961 accgccttcc ccctggatgg gttcgacaat caccgcacag gtggcgtcgt caatcagcgc
    10021 gctggcagag ttaatatcgt tatatgcagc atgacggata tctggtggca gtggcgcaaa
    10081 atcctgtgaa taggctggct gcccacccgc actgacagta aacagcgtgc gaccatgaaa
    10141 cgcattttta aacgccatga taccgctctt atggctgccg tagcggtcgt gagcgaattt
    10201 acgcgccagt tttagcgccg cttcgttggc ttccgcaccg gagttacaaa agaagacgcg
    10261 atcggcaaac gtggcgtcga tcaatttttt cgccagtcgc agtaccggct cgttggtgta
    10321 accgttgccg gtatgccaga acttactcgc ctgttcgttc agcgcttcac gcagttccgg
    10381 atgcgcatgg cccagcgcgt tcaccgcaat gccacccgcg aagtcgatat actctttccc
    10441 ctgctgatcc cacaagcgcg aaccttcgcc acgtaccggt ataaagggtg ccggagcgta
    10501 aacaggtatc atccattcat caaagttttc acgcgtaatt ggctgagaca tagcgacctc
    10561 tacagtaaat aattcgttat ttatatgtta acaataagta atgtttgcgc tgtaaatgta
    10621 gattgcaggg ttcgtgccag ccagtgataa aagtgcataa acggcggagg ctaactggaa
    10681 atcaaggagt tataaccaaa ccatatgcat ttaaagtgca tataaagtga atacgtttgc
    10741 gatgtgggtg aataaaaaga ataaaaaacg caatgttatg cagaagtaaa atataattct
    10801 ggaattgtga tcattgacga aatttactgg aaattactgc gccattctga cgcagcgcgc
    10861 accaaaagcg ggcatttttt gcgccatcgt tgacatcatt aacaaccatc gatcaaatca
    10921 cttaacaaca ggcggtaagc aacgcgaaat tctgctacca tccacgcact ctttatctga
    10981 ataaatggca gcgactatga aatttgtctc ttttaatatc aacggcctgc gcgccagacc
    11041 tcaccagctt gaagccatcg tcgaaaagca ccaaccggat gtgattggcc tgcaggagac
    11101 aaaagttcat gacgatatgt ttccgctcga agaggtggcg aagctcggct acaacgtgtt
    11161 ttatcacggg cagaaaggcc attatggcgt ggcgctgctg accaaagaga cgccgattgc
    11221 cgtgcgtcgc ggctttcccg gtgacgacga agaggcgcag cggcggatta ttatggcgga
    11281 aatcccctca ctgctgggta atgtcaccgt gatcaacggt tacttcccgc agggtgaaag
    11341 ccgcgaccat ccgataaaat tcccggcaaa agcgcagttt tatcagaatc tgcaaaacta
    11401 cctggaaacc gaactcaaac gtgataatcc ggtactgatt atgggcgata tgaatatcag
    11461 ccctacagat ctggatatcg gcattggcga agaaaaccgt aagcgctggc tgcgtaccgg
    11521 taaatgctct ttcctgccgg aagagcgcga atggatggac aggctgatga gctgggggtt
    11581 ggtcgatacc ttccgccatg ctaatccgca aacagcagat cgtttctcat ggtttgatta
    11641 ccgctcaaaa ggttttgacg ataaccgtgg tctgcgcatc gacctgctgc tcgccagcca
    11701 accgctggca gaatgttgcg tagaaaccgg catcgactat gaaattcgca gcatggaaaa
    11761 accgtccgat cacgcccccg tctgggcgac cttccgccgc taatttagca gctctcctgg
    11821 ctcaaactgg gtcaggagaa ttaaccttga gaaaaatcaa caaactgtca gtaatgattt
    11881 gttgcctgcc gtcctttgtt ataccgtcac tgcgttttta gttgtctgac cacttctcta
    11941 ttatcaagtt tgatatagga aactccacga tgaatgctga gcgtaaattt cttttcgcct
    12001 gtcttatttt tgcgctggtc atttacgcta tccacgcttt cggtttattc gatctgctca
    12061 ccgatttacc ccacttacag acactcattc gccagagcgg attgttcggt tatagcctct
    12121 atattctgtt attcatcatt gccaccctct ttctgttacc aggaagcata ctggtgatcg
    12181 ctggaggaat agtttttggc ccgctcttag ggacactact ctcattaatt gccgccacgc
    12241 tggcctcctc gtgctcattc ctgctggcgc ggtggctggg acgtgattta ctgctgaaat
    12301 acgtgggcca tagccatacc ttccaggcca ttgaaaaagg cattgcgcgt aacggtattg
    12361 attttcttat tctgacccgc ttaatcccgt tgtttcctta caatattcaa aattacgctt
    12421 acggattaac caccatcgcc ttctggcctt atacccttat ttcggcac
//
LOCUS       DAJTSL010000122        12154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTSL010000122 DAJTSL010000000
VERSION     DAJTSL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.122"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(34..1743)
                     /locus_tag="OO433_003020"
     CDS             complement(34..1743)
                     /locus_tag="OO433_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6051416.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDAHLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(1864..3255)
                     /gene="xanP"
                     /locus_tag="OO433_003021"
     CDS             complement(1864..3255)
                     /gene="xanP"
                     /locus_tag="OO433_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6051417.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKTWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITSTSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            3535..4740
                     /gene="gltS"
                     /locus_tag="OO433_003022"
     CDS             3535..4740
                     /gene="gltS"
                     /locus_tag="OO433_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6051418.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(4774..6855)
                     /gene="recG"
                     /locus_tag="OO433_003023"
     CDS             complement(4774..6855)
                     /gene="recG"
                     /locus_tag="OO433_003023"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6051419.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(6861..7550)
                     /gene="trmH"
                     /locus_tag="OO433_003024"
     CDS             complement(6861..7550)
                     /gene="trmH"
                     /locus_tag="OO433_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6051420.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(7557..9665)
                     /gene="spoT"
                     /locus_tag="OO433_003025"
     CDS             complement(7557..9665)
                     /gene="spoT"
                     /locus_tag="OO433_003025"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6051421.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(9684..9959)
                     /gene="rpoZ"
                     /locus_tag="OO433_003026"
     CDS             complement(9684..9959)
                     /gene="rpoZ"
                     /locus_tag="OO433_003026"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6051422.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(10014..10637)
                     /gene="gmk"
                     /locus_tag="OO433_003027"
     CDS             complement(10014..10637)
                     /gene="gmk"
                     /locus_tag="OO433_003027"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6051423.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     DEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            10895..>12154
                     /gene="ligB"
                     /locus_tag="OO433_003028"
     CDS             10895..>12154
                     /gene="ligB"
                     /locus_tag="OO433_003028"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6051424.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRQQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     SVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVSL"
BASE COUNT         2709 a         3189 c         3328 g         2928 t
ORIGIN      
        1 gtacgtctga gtcgtacccg gcgagattga tgtttactgc accagctctg ccgacgtaac
       61 ttcaccgctg gaaacgatgc cagcattcat ggtttgagtc acttgctgct ttgcggcgtt
      121 cacggcatgc aattgcccgc taaccgtagg tttcagcggg gcattggcct gaatatctcc
      181 actggcggta agctgaatat tgccgtcgcc agtcaacggt aattcaggcc atccccattg
      241 ttgcaaaata ttcaccggca caccgcgtcc attcaggctg atatgggtct gacgttgtgg
      301 cgtttgtgaa acactggcag tggcttccag aatgcctttt tcagtaaatg cactcagttc
      361 gctgatattc accgtgctgc tgttggcggt cagcgccagc gacgggcgac gaacatcaac
      421 acgattgaat gtcgcggcgg cggcattcag attcgccgag ccactccaga cgccccattt
      481 atgatcggta accagcgtca ggttggcacc gtaaccatcg agcgtggtga gctgccacgg
      541 gaagtcaggg tcgatatcaa tgatcagatt gcggctggcg ctaaatctct tcagttgcag
      601 gctgtttaac caaccgggtg tcgtttccat ccacaactgt tgccagtttt tcggcaaggt
      661 atattccagc ccggcaattg ccgcatcatc aaggatcaac gttttcccgt cacgcagcca
      721 gttccctgac gttctgacca tacccccttc ccagcggctg gtgaactggc gcagcgctac
      781 gccctgcggg gaaaattccg cgtttataat cgggtcaaat aaatgcagcg aaccataaat
      841 gaactcgcta gcgttcatcg acagtttgcc ttcttgtgtc tgccagtcat ctttactgaa
      901 ggtcatgttg cgcaagctga gatcgaggtc ggtcaccgcc cagtccggac cttgcaaatg
      961 agcatcgatc acttccaggc gaccaatctg caacgacggg acagagcgta atggcgcaaa
     1021 gaagtcggtt agcgattttt cgctttgtag acgtatatcc gccatgcgca ggttttccac
     1081 ttgccagctg ccgtcggcgt tacgctgcgc gtttccggtt aatgtcccgc gggcgatgtc
     1141 ggcacccagg ttagtcagcg taacgcgatc gttatcaata ctgccttcaa tcagtacatt
     1201 ggtggcagga acatcattga gcgaaagcga tccggcacta aactgaatct gcgccttcgt
     1261 acccagcact ttaccggctt ttggtgacca cggaactacg ccgccattta cccgctgcgc
     1321 gctcagtttc cattcgctat tcgggctatt aaacgccata tcacgcagtt gcagacgatc
     1381 ggctttgaac ggtagcggcg cggtctggtc ggtgagattc agcgtcccgt tttccagcag
     1441 gatggtatcg acatggcgtg gttcggtcag ttgccgactg cttagcgcaa tgtcgacact
     1501 tttggccacc agggtcgcgg gctgaccatc acgaccaaac gtgacattct ccagcacgat
     1561 atgagatggc gcggaaaaac ggtgatccat cgccccgaag gccagatgat agtcgctatt
     1621 ctcggaaacc catgcgctga tatgttctgc tccccagcgg gtttgcagaa gaaaatagag
     1681 gccagcgatc accactaaca gagcgatgag aatgtagaga agcagcttcc caataaattt
     1741 catggtcttc cattccgtga aatgcacata agagagttat gcacgattta cgcgcaatcc
     1801 tcaaggcggg aatggtgaaa gaggtgagac tgcggcggta attatcttac cgccgccagt
     1861 gaattactgt ttttctggtg ggaaaatcag attcagcacg atggcagtaa taccgcccgc
     1921 ggcgatcccg gaggagagca ggtttttcag ccattcaggg gcaaactgca aaatcagcgg
     1981 ctgctgagac acgcccagac caaccgccag cgacagcgcg ataatcagaa tcgcccgacg
     2041 gttcagcggc tcacgagaaa cgatacgcac accggaggcg gcgatggtgc caaacattac
     2101 aagcgttgcg ccgcccagaa ccggttctgg aatgtgttgt acaaaaccgc tcactgccgg
     2161 gaacagaccc agcacgatca acatcagcgc gacgacaaaa ccgacatagc ggctggcaac
     2221 accagtcaac tggatcactc cgttgttttg cccgaagcag gagttcggga aggtgttaaa
     2281 caccgccgaa acaaacgagt tcaggccgtt tgccagcacg ccgcctttca ggcgtttcat
     2341 gtacagcgga ccggacactg gctgttcgga aacgtcagag gtcgacgtga tatcgccaat
     2401 ggtttccagc gaagtgatca taaagaccag catcagcggc agcagcagac tccattcaat
     2461 gccaagaccg taatagagcg gcgttggcac cataatcagt tcttgcgtca tcggttcgtt
     2521 gctttctggc aacatgccca taaaccacgc cagcgcatat ccggccgcca tcgcaatcac
     2581 cagtgaggcc acgcgtaagt aagggttacg ttgacggtta agcaggataa ttaaggctaa
     2641 gaccacgcct gccagcagca gatttttcgg tgcgccgaag gtgttatcgc tcatggctgc
     2701 gtaaccgccg ccaatggacg ttaacccaac ctgaattagc gacaggccga taatcatcac
     2761 cacaacgcca gaaaccagcg gcgtaataat gcggcgcgcc agatgcagaa cgcgggagat
     2821 caccatctcg gtgcaacttg ccagcatcaa cgtgccgaac aaagccgcca tcatggtagg
     2881 aacatcagca ccaccggttt tcagcgcggt accgcccata atcagcgggg caacaaagtt
     2941 gaagctggtg ccctgaatag acaacagccc ggagccaacc ggaccccagg tcttaatttg
     3001 aataatcgat gccacaccgg aggcaaacag cgacatacta ataatgtgtt gcgtgtcttg
     3061 tgccggtaaa cccagcgcct ggcagattaa tagcgctggc gtgatcaccg caacgaacat
     3121 cgccagcaga tgctgacagg cggcaaacag ggtttgagga agcggcggac gatcttcaag
     3181 acggtaaatc agttcgctgt tttgagtctg cgcaaccggt tgcgcatttt ctgactcgag
     3241 ggtggaaaca gacatcgaaa acaatcccgt ggtagcaaag cgggcatttt agctgaccga
     3301 aagagaaaag caaacgtttg ccacatcctc atcaaaatat ttaaatgaga taaatgcact
     3361 tttttatata acttttggta tttttctgct taaaatccat gccatttgtt cattaacagc
     3421 tgagaatttg acccaggaaa gcataactcg atacccgtca tagttcatga agttgccact
     3481 gcgcgatgaa gtatgacgag tatgaaagag tgatgcggat acaaaggagt aactatgttt
     3541 catctcgata ctttagcaac gcttgttgcc gcaacgctga cgttgctgct cgggcgtaag
     3601 ttggtccatt ccgtctcctt tttgaagaaa tacaccatac cggaacctgt tgcgggtggt
     3661 ttgttggtgg cgctggcgct actagtactg aaaaaaagca tgggctggga agtcaacttt
     3721 gatatgtccc tgcgcgatcc gttaatgctg gctttcttcg ccaccattgg cctgaacgcc
     3781 aacattgcca gtttgcgtgc cggtgggcgt gtggttggca tcttcttgat tgtggttgtt
     3841 ggtctgttgg tgatgcaaaa tgccattggc attggtatgg ctagcctgtt agggcttgat
     3901 ccgctgatgg ggctgttggc cggttctatt acgctttccg gcggtcacgg tacgggcgct
     3961 gcgtggagta aattgttcat tgaacgttat ggcttcacca atgcgacgga agtggcgatg
     4021 gcctgtgcaa cgttcggtct ggtgctgggc ggcttgattg gcggtccggt ggcgcgctat
     4081 ctggtgaaac actccaccac gccgaacggt attccggatg accaggaagt cccgacggcg
     4141 tttgaaaagc cggatgtggg acgcatgatc acctcgttgg tgctgattga aactatcgcg
     4201 ctgattgcta tctgcctgac ggtggggaaa attgttgcgc aacttttggc tggcactgct
     4261 tttgaactgc cgaccttcgt ctgtgtactg tttgttggcg tgattctgag caacggtctg
     4321 tcaataatgg gcttttaccg cgtctttgag cgtgcggtat ccgtgctggg taacgtaagc
     4381 ttgtcgttgt tcctggcgat ggcgttgatg gggctgaaac tgtgggagct ggcttcgctg
     4441 gcgctgccga tgctggcgat tctggtggta cagaccatct tcatggcgtt gtatgccatc
     4501 ttcgttacct ggcgcatgat gggcaaaaac tacgatgcgg cagtgctggc tgcgggtcac
     4561 tgtggttttg gcctcggtgc aacgccaacg gcaatcgcca acatgcaggc gatcactgaa
     4621 cgctttggcc cgtcgcacat ggcgtttttg gtggtgccga tggtcggtgc gttctttatc
     4681 gatatcgtca atgcgctggt aattaagttg tatttgatgt tgccgatttt tgccggttaa
     4741 ccgatgaagc ggcggtcttc tcaccgccgc cttttacgca ttcgagtaac gttctgtctc
     4801 cggcatccag cgttctatca aggcttttgc ctgttgtggg taacgttcgt gaatatgtcg
     4861 cgccaggcgc tgaacttccg ggatcatcgc ctgatcgcgc agtaaatccg ccactttaaa
     4921 ttcagcatta cccgtctgac gcgtgcctaa caattcgcca gggccgcgaa tctccagatc
     4981 tttttgcgca atcacaaaac cgtcgttact gtcgcgcagc acttgcaggc gaatttgcgc
     5041 cgttttagaa agcggcgttt tgtagagcag cacgcagtga gaagccaccg cgccacgacc
     5101 tacgcgcccg cgcagctggt gtaactgcgc cagacccaga cgctcagggt tttcgataat
     5161 catcaggctg gcgttaggca catcaacacc gacttcaata acggtagtgg caaccagcag
     5221 atgtagctca ccttgtttaa acgacgccat caccgcctgt ttctcggctg gtttcatccg
     5281 cccgtgtacc aggccaacat tcaattctgg tagcgccagt ttcaactctt cccaggtagc
     5341 ttccgccgcc tgcgcttcca gtaactctga ctcttcaatc aacgtacaaa cccagtacgc
     5401 ctgacgacct tcagttatgc aggcatggcg cacgcggtca atgatgtcgg tacggcgggt
     5461 atcaggaata gcgaccgtag tcactggcgt gcggcctggc ggcagctcat ctatcaccga
     5521 ggtatcgaga tcggcatacg ccgtcattgc cagcgtgcgg gggatcggcg tggcggtcat
     5581 gatcaactga tgcggatgga agccttgctg ctggcctttc tcccataacg ccagacgctg
     5641 atgcacgcca aagcgatgtt gttcgtcgat aatcaccagc gccagaccgt taaactgcac
     5701 ctgttcctgg aagatggcgt gcgtaccaac aatcatctgc acctgaccgc tggcgatggc
     5761 ttcctgctga gacagtcgcg ctttgccttt ctgcttaccg gcgagccagc ccacttcgat
     5821 accgagcggt tcaaaccagt tgcggaagtt attggcgtgc tgttcggcga gtaattcggt
     5881 tggtgccatc aatgccacct gtttgccgtg ggcaatcgca cgcagcgcgg cgagggcggc
     5941 gaccagcgtt ttaccggaac ctacatcgcc ctgtaccagg cgcatcatcg gcacatccag
     6001 cgccatatcg cgctcgatct ccgccactac gcgtgcctgt gcgcccgttg gcttgaacgg
     6061 taaggctgcg aggagtttat ttttcagcgc atcattggcg ctcagcggct gggcatgaaa
     6121 acgctgtgct ccggcacgta aggctaacat gctgaggttg tgcgccagca gttcttccag
     6181 aatcagacga cgttgcgccg gatgctgccc ggtttccaga tcgctaagct gtagcgtcgg
     6241 tggcgggcgg tgcaaagtgc gcaacgcttc cggtagcgtc atcattcctt gtgacagttc
     6301 cggcggcagg agttcttcaa tggcgcaggt gtcgagcaga tccagcgcct ggtcggttaa
     6361 tttacgcagc gtggcctgct ttacgccttc cgttgttgga taaaccggcg tgagcgtttc
     6421 ctgtaattct ggcgtgctga gatcgccctg cacgcggtat tccgggtgga tcatctccgc
     6481 accatattta ccgcgctttg cttcgccata agccagtaca cggcggcccg tcgccaggct
     6541 atttttcatt gccgcgctga aattgaaaaa gcgcatggtg aggatgccgg aaccgtcgct
     6601 gatctggcag gtcatcatcc gccgaccgcc gaaggagata ttgcagttca gcacttcgcc
     6661 ttccaccgtg gcataaacgc ccggcagtag ttctccgatg gggtagagat gggtgcgatc
     6721 ttcgtagcgc aggggaaggt gtaagagtag atcctgcacg gtatgcaggt tgattttcgc
     6781 cagtttgtta ctaagtgctg cgccaacgcc cgttagggaa ctgagtggga cagcatctaa
     6841 caggcgacct gtcatggcac ttaccctgca gcctgcatag tagcccacca gtcggcatca
     6901 gcttcgatct cgccttgctg attgacgtgg ggataaggca ggcctttgcg ttttgcgact
     6961 ttcgccagca ccggatagcc gccttcaaac aacaggcgtt gttgctctgc ttccggcaac
     7021 atgctgtttt cacgcaggta catgcctgca ttttgccgct gacgctgggc ttcgtaaaga
     7081 atgagggctg aggcaacgga aacattcagc gactgcacca tgccgatcat cggaatgatg
     7141 atgtcctgat ccgccagggc caatgcttcc tgcgtgatgc ccgttttctc ctgtcccatc
     7201 aaaatgcagg tcgggcgagt gtaatcaatt tcgcggaaat cgacagcgtt atcagaaaga
     7261 tgggttgcca gaatctgcat gccctggcct ttgagatgag cgacggcatc gccaatggtg
     7321 cggtgtgttt tcacctgtac ccagctgtta ctacccgccg ctgccgaagc catggtgcgc
     7381 atgcggctac caggccagac ggcgtgaact tcatgtacgc caacggcatc tgcggtacga
     7441 ataatcgcag aaacgttatg aggtttgtgg acctgctcca tgcagacggt cagatcaggc
     7501 tgccgcctgg cgagcatttc gcagatgcgt gcataacgtg ttgggttcat aaaacattaa
     7561 tttcggtttc gggtgacttt aatcacgtct ggcatcacgc ggattttgcg catgatattc
     7621 gccagatgca cacggtcacg agcggtcaga cgaataaagg cgctgtagac gcgaccatct
     7681 ttctcttccg tattcaaact ttgaatattc gaagtcgtgg tgttaattgc cgccgtcagg
     7741 tttgccagcg caccctgatg attgaacatc tccaccttga tttcggtgat gaactcctgc
     7801 gccgtctctt tatcccattc cacagccata aacttctctg gctctttctg gtagccacgg
     7861 atgttacggc aggattcatg gtggatcacc agacctttac cggggctgac gtgggcgata
     7921 atcgggtcgc caggaatagg gcggcagcat ttcgcaaagg tgatcagcac gccatccgcg
     7981 cctttgatcg gcagatgccc gtggctttga gtcgccggtg gaatggaggc gtccccatgt
     8041 tgtagatttt tcgcgaccac cacgctcatt gcgttaccaa ggccgatttc tgccagcaga
     8101 tcgtcaagcg ttgccagctt catgcgatcc agctcgcgct gaatattttc ctgcgggatt
     8161 tcattgagct tacggctacc acccaaagca tggttgagca gacgacggcc caggcttaca
     8221 gaatcatcac gcttgaggtt tttcagcaac tgacgaattt tggcgcgcgc tttcgagcta
     8281 acgacaaagt tcagccaagc ggcattcggg cgagcgcccg gagcggtaat gatttcaacg
     8341 gtctgaccgc tagtaagcgg ctgcgacagc gggtaaggct ggcggtcgac gcgtgcgccc
     8401 acgcaggcat ggccgatatc ggtatgcact gcataagcga agtcgacggg cgttgcaccg
     8461 gcaggcagct cgacaatgcg cccttccggt gtgaaaacgt aaatctcatc cgggaagaga
     8521 tcggatttaa cgctctcgat aaattcaaac gaactaccgg cgctctgttg cagctccagc
     8581 aggctttgca tccagcgctg ggcgcgtatt tgtgcggtag tactggtttc gccgtgctct
     8641 ttataagccc agtgcgcggc aacacccatc tccgccatct gatccatatc ttcggtacgg
     8701 atctggacct caaccggcac gccgtgcggg ccaatcatcg aggtgtgcaa cgactgatag
     8761 ccgttcgctt ttggaatggc gatatagtct ttcacgcggc ccggacgcgg cttgtacagg
     8821 ctgtgcatct ggcccagcac gcgataacag gtgtcagaat cattgacgat cacgcggaaa
     8881 gcgtagatgt ccatgattga gtgaaaacgc tgctctttga gcaccatttt gcagtaaatc
     8941 gaataaagat gcttttcgcg accactgacg cggcacggta ttcccgcttc ctgcaaacgc
     9001 ccttcgattt cagaaagaat cttctggatc atctctttac ggttgccgcg cgcggctttc
     9061 accacttctt tgattacgcg ataacggttg ggatacagcg cctcaaaacc cagctcttcg
     9121 agttcggttt taatgtggtg gatacctaaa cggtgcgcca gcgggctgta aatttcgaga
     9181 gtttcacggg cgatgcggcg acgtttgtcc gggcgaagtg agcccagcgt gcgcatgttg
     9241 tgggtacggt cggcaagttt gatgaggatg acgcggatat cctgcaccat cgccataatc
     9301 atcttgcgaa agttttcggc ctgcgcctct ttcttatcgc ggaacttgag tttatcaagt
     9361 ttcgacaccc cctctaccag ctcggcgacg cttttaccaa aaagctgttc catatcctgg
     9421 taggtggcgg gagtatcttc aatcacgtca tgcagcagcg ccgccatcag cgtttcatag
     9481 tcgagtttca tctcggccag aatgcaggca accgctaccg ggtgcgtgat atagggttca
     9541 ccgcttgaac gtgtttgccc ctcgtgagca tcacgtgcaa cgagatacgc ctgccgcaga
     9601 cgcttgattt ggtcttccgg caggtaggtt tgaatcagtt gattcaggct ttcaaacaga
     9661 tacaagggcg acccgctttg tgattaacga cgaccttcag caatagcggt aacggcttgt
     9721 aattcagcgg cttcctgctc ttgctgttcc tggcgttcgc gaacgtcgag gatctggttg
     9781 ttgatcagac cttcttcgat ttcgcgcagc gcgattacag tggttttatc gttttcttcc
     9841 ggtaccagcg gatcctttcc gcctacctgc atctgacgag cgcgacgcgc ggcgaccagt
     9901 accaggtcaa aacggttacc aattttctct acagcgtcct gaacagttac gcgtgccata
     9961 cttaaaaagc tccacaggtg aagaaatgac tgggcatgat actgaaatca ggttcagtct
    10021 gccaacaatt tgctgattaa agcgtcatga cgctgctttt ggcggctcat gcgcagacgt
    10081 tcggcgcgaa taatggtctt caaatcggtc aacgcggtat cgaagtcatc attcacaatc
    10141 aggtaatcat attcgtcgta atggctcatt tctgcaacag cttgcgccat acgctttgca
    10201 atgacctctt cgctgtcctg accgcgaccg cgtagacggc ggtccagttc aattttagac
    10261 ggcggtaaaa taaagatact ccgcgcgtgc ggcatcttct ggcgaatttg ctgcgcgccc
    10321 tgccagtcga tatcgagaaa aacatcgaca ccggtcgcca gtacttgctc aatggcctca
    10381 cgcgaagtgc catagtaatt accaaaaact tctgcgtgtt cgaggaacgc atctctgcta
    10441 atcatttctt taaattcatc atgattaaca aagaaataat gttcaccgtg gacttcacca
    10501 ggacgcggtt ggcgtgtggt gtgtgaaaca gaaacctggg tgtcatacaa cggttgggtt
    10561 tttaataaag cctgaatcag gctggattta cccgcgccac tgggggcaga aacaatataa
    10621 agcgtgcctt gagccatgag tatctttcgt atgtgattag cgaaataaag cctacatacg
    10681 agcttattat acacggcgcg gccatgtgac gtagcccttg ctcacacttt ttgcgcaaag
    10741 ttgttgcgtt ttgcgtagct gcttccgcat ttgcctgtga tttactgcaa taaagacgat
    10801 tctcaggaag cgtcctcgca gactccgttt ttgccactcg gctttgcctt caccacgttg
    10861 tatatctgcc agcctgacta cagcggaggt gaagatgaaa gtatggatgg cgatattaat
    10921 aagtatcttg tgctggcaat catctgtgtg ggcggtctgt ccggcctggt cgccagccag
    10981 agcacaggaa gaaattttcc gccagcaaca gcaaataaaa cagtgggacg atgactactg
    11041 gaaggaagga aaaagtgagg tggaagacgg tgtttacgat cagttaagcg cccgtcttac
    11101 gcagtggcaa cgctgctttg tgagcgagcc ccgcgacgtg atgatgccgc ctttaaatgg
    11161 tgcggttatg catccggttg cccatacggg tgtacgcaaa atggcggata aaaacgcatt
    11221 aagtctgtgg atgcgagaac gtagcgatct ttgggtgcag ccaaaagttg atggcgttgc
    11281 ggtaaccctg gtttatcggg acgggaaact gaacaaagca atcagtcgcg gtaatggcct
    11341 gaaaggcgag gactggacgc agaaagttag cttaatttcc gctgtgccgc aaaccgttag
    11401 cgggccttta gccaacagta cgcttcaggg ggaaatattt ctccagcgcg aggggcatat
    11461 ccaacaacaa atggggggaa taaatgcccg cgcaaaagtt gctggcttga tgatgcgcca
    11521 ggacgatagc gacacgctga attctctggg cgtttttgtc tgggcatggc cggatggacc
    11581 gcagttaatg tctgatcgtt taaaagagct ggctaccgca ggttttactc taacgcagac
    11641 gtatacccgt gcggtgaaaa atgctgatga agttgcgcgc gtacgcaatg agtggtggaa
    11701 agcggaatta cccttcgtca ccgatggcgt agttgtacga gcggcgaaag agccagaatc
    11761 ccgccattgg ctaccgggcc aggcagagtg gctggtggcc tggaaatatc aacctgtagc
    11821 tcaggttgcc gaagtgaagg cgattcagtt tgcggtgggt aagagcggta aaatatcggt
    11881 ggttgcgtca ctcgcatctg tcatgctgga tgataaaaaa gtccagcggg tgaatattgg
    11941 ttccgtcagg cgctggcagg agtgggatat tgcgcctggt gatcagattc tcgtcagcct
    12001 tgccggtcag ggtattcctc gcattgatga tgtggtgtgg cgcggtgcag aacgtacaaa
    12061 accgacaccg ccagaaaacc gctttaactc gttgacctgc tactttgctt ctgatgtttg
    12121 tcaggaacag ttcatttcac gcttagtcag tctg
//
LOCUS       DAJTSL010000123        12054 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTSL010000123 DAJTSL010000000
VERSION     DAJTSL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.123"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..192
                     /locus_tag="OO433_003029"
     CDS             <1..192
                     /locus_tag="OO433_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6051425.1"
                     /translation="RGAFFISALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGT
                     NTLLACCCLLRPEEVNSEY"
     gene            182..355
                     /locus_tag="OO433_003030"
     CDS             182..355
                     /locus_tag="OO433_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6051426.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(404..1476)
                     /locus_tag="OO433_003031"
                     /pseudo
     CDS             complement(404..1476)
                     /locus_tag="OO433_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1548..4292)
                     /gene="torS"
                     /locus_tag="OO433_003032"
     CDS             complement(1548..4292)
                     /gene="torS"
                     /locus_tag="OO433_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCR6051427.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPMLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSTVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4375..5364
                     /gene="torT"
                     /locus_tag="OO433_003033"
     CDS             4375..5364
                     /gene="torT"
                     /locus_tag="OO433_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6051428.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSF
                     PDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMP
                     GPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAG
                     TAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMIWQGELAVEQA
                     IRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(5337..6029)
                     /gene="torR"
                     /locus_tag="OO433_003034"
     CDS             complement(5337..6029)
                     /gene="torR"
                     /locus_tag="OO433_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6051429.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQT
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGECYFLAADVC"
     gene            6149..7321
                     /gene="torC"
                     /locus_tag="OO433_003035"
     CDS             6149..7321
                     /gene="torC"
                     /locus_tag="OO433_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6051430.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDIAGKAHGDKKEEK"
     gene            7321..9893
                     /gene="torA"
                     /locus_tag="OO433_003036"
                     /pseudo
     CDS             7321..9893
                     /gene="torA"
                     /locus_tag="OO433_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            9890..10489
                     /gene="torD"
                     /locus_tag="OO433_003037"
     CDS             9890..10489
                     /gene="torD"
                     /locus_tag="OO433_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6051431.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNK"
     gene            complement(10582..10887)
                     /gene="cbpM"
                     /locus_tag="OO433_003038"
     CDS             complement(10582..10887)
                     /gene="cbpM"
                     /locus_tag="OO433_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6051432.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(10887..11807)
                     /gene="cbpA"
                     /locus_tag="OO433_003039"
     CDS             complement(10887..11807)
                     /gene="cbpA"
                     /locus_tag="OO433_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6051433.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2894 a         3094 c         3191 g         2875 t
ORIGIN      
        1 cgtggagcct tttttatatc tgccttattt ttcttcaacg ctgtatgtat agtaagcgat
       61 aacctgttga ttattgaatc tttcggggag atggcttata acatttctta cctgaccagg
      121 gtaccgggaa ccaacacctt actggcgtgt tgctgtcttt taagaccaga agaggttaac
      181 agtgaatatt gaagagttaa aaaaacaagc cgaaacggaa atcgccgact ttatcgcgca
      241 aaaaatcgcc gagctgaaca agaatacagg gaaagaagtc tctgaaattc gcttcaccgc
      301 acgagaaaaa atgaccgggc ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg
      361 tgaacatcac cccgtgcgag gtgatgttcc gcttgttgct aatttagtga tcaatcattg
      421 gcgcttgtgg aattaagcgt cggtacaatt cctccggcac cgggctttgc catactcccg
      481 catacattgc gtaaccaatc accgcgaaca taatccccag aaccagtagc gtcattaacc
      541 agccagacaa cgcaaaagct tttttatttg ccgcaggttt ttgcagtgaa aaggtcaatg
      601 ttgaggctac cggacatgac tctacgcaag tcatacagcc ggtacattcc actgttcgta
      661 cctgaattaa tttatctacc gggatccgtg atgggcaatt ttttgcgcat ttgccacagc
      721 cgatacaact ttcggcattg cgacgaatct taaacggcga taatagcgaa accacgccca
      781 tcagcgcgcc atatgggcaa agataacgac accaggcatg gcgaataaac aggctggcaa
      841 tcagcaaaac ggtcacgctg attaatgtcg cggtccccat atgacgaaag aaatcgagca
      901 tttttacgtc catcaccacg ctgtagggcg acaacataaa atagtgaatc gcctgagcgg
      961 gcatcaataa cgcgatatag agaaaaaaac tcaacagcaa atacttcacg ccgcgcagag
     1021 gaatatccag ccagcgggga aggacacatt gccgaccaaa cagtttgtta ccgagatcgc
     1081 cgattaattc agaaagcgta ccaaccgggc ataaccatga gcaaaaggcc tttttgagta
     1141 atagactgat gacgataaaa gcgaccaata acagcatcgc ggagcgtgga cggacggtaa
     1201 ctgacctgtt gtaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc
     1261 gatacctccc ggtctggcga caaatgtcgt gctacttgcc gtttcgtaat agcgcaccca
     1321 ataccagaac gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt
     1381 acgccaggtc gtggcattgc gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg
     1441 ccgccgctgc cagcgggttc tttttttctc tgccatgatt ttgccagtcc gttaagttgc
     1501 ataccaaatg ccactatttt agttgttctt aactggctga tattgattca aatcgcgttc
     1561 aggtctttct tatgcaacca tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt
     1621 ggaagggggg cagataatgg ctgctgctcc agttgtgcgc atagctggct ggcgatacgc
     1681 attcccaaac tcgagcaact gctttttagc tgatgcgcgg cacgctttat tttctcgcta
     1741 tcctgactgg cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat
     1801 aataccagcc attcgtggat cttctccgtc cccattaact gagcatcttc attgagttgc
     1861 gatacatcca gcgattgatc gttattgact tgcagttgga gatagtgcgc cagtaactga
     1921 ccgagcactt cacgcggcac cggtttaggg ataatcccgc ggaataatga actggtacgc
     1981 tggcgcagcg tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag cgacggatat
     2041 tgctgtgcca gttgtcgggc aagcgtaatg ccgtcgacat ccggcagatc aaaatccacc
     2101 agtgcggcag caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca
     2161 acagcaacaa cctgcgcacc actggtgttc agcatctcaa cggtaattcg ctgggttagc
     2221 gggttatctt caattaacag caaacgtaaa ccgtcaagac gcaccgcctg attgactgtt
     2281 tttggcacgg gtgccgtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc
     2341 tccggcgtgc tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata
     2401 gtcagtccca gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg ctggaagatt
     2461 tctgccagtt tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc
     2521 tcgccatcag tgcggctacg taaaacgatc tgcccttcgt cagtaaaacg cagggcgttg
     2581 ctcaacaggt tggttataac ctgacgaata cgtcgcggat cgcccattaa cgcggtcggt
     2641 acatcgtcgg caattgctgt tgccaggcga atcgggcgac ctttcacccg tccgctcatt
     2701 aattgcaggg tactttccag cagcgggcgc ggttcaaagg gctcatcgct gaccgaaaca
     2761 ttcttgccac ctgcttcgat agccgaataa tcgagaatat cgttgaggat ggtcagcaac
     2821 gattcgccag agtcggtaat cgcccgcaaa tcatcacgct gggcgttaag tgcggggtta
     2881 tctgccagca gttgagcagt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc
     2941 atcgccgcca gaaaggccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcctgt
     3001 cggtgttcta tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc
     3061 tgttcacggt ggcgattcag tgcatgaaca gtgctgcgaa acgcatccat cagccgcccg
     3121 atggtatcca gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc
     3181 agcagccgtt gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc
     3241 acgcgccaaa ggatcagaat cagtgcgcaa agtgaaacca tccccagcaa taacaggctg
     3301 tattgcccgc gtgcactggc tttttccaga tgcgccagtc cgtgctgatt acgcagctca
     3361 atggtgtcga ccagctgact gacttcgcta ctaaactgcg cgaactgggc gatgttattt
     3421 tgtgcgagag tttgtaggtg attgctgatt tcactgtcct gctgatacag cgccagcaaa
     3481 tcgctatatt ggctaacggt agttaacgtt gttgcgacct gcgcacgaac acccggatct
     3541 tcaatgcgta tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc
     3601 attggtgcat ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc
     3661 agagcgctaa ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc
     3721 cgatcgagtg cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg
     3781 gcctgggtcg ctccagcgga agttgtcgca ttattcgcct gaccttgcgc caggcgtgcg
     3841 atctcatcgg cggcagcgac tatctgctga ctgagttgct gttgttgctg gcgtagttgc
     3901 agacgccgcc ccaccagttc cccttgctga cgtaacgaac gggagatctc ctgctcctgt
     3961 tgttcaatag cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc
     4021 ttcaggcttt gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca
     4081 ctggtcaggt tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc
     4141 atattcatgg taggaatcaa tgcctgagtg ttgtcttttt ccacctggct gataaagcgc
     4201 aggttgtacc atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc
     4261 atccagagtc ttcgggtcag ggttaaattc acggtcggtg cactttaggt gaaaaaggtt
     4321 gagtcgcaaa gcagaatgca tctagcataa agccttatta ttgattaggc tatcatgcgc
     4381 gtactgctat ttttacttct ttcccttttc atgttatcgg cattttcggc tgataacctg
     4441 ttgcgctggc atgatgcgca gcattacacg gtgcaagcct ctacgccgct taaagccaaa
     4501 cgcgcatgga aactgtgcgc gctttatccc agcctgaaag attcatactg gttatcgttg
     4561 aactatggta tgcaggaggc aggcggctac agccagttgg ctacccagca agcacaaatc
     4621 gaccagtgta aacagtgggg cgcagaggcc attttgctcg gtagtagcac tacgtcattt
     4681 cccgacctgc aaaagcaggt agcaaatctg ccggtgatcg aactggtaaa tgctcttgat
     4741 gatcccctgg tgaaaagccg cgttggtgta ccctggtttc agatgggcta tcaacctggg
     4801 cgatatttgg tgcaatgggc gcacggtaaa ccactgaacg tgctgttgat gcccggaccc
     4861 gataatgccg ggggcagtaa agagatggtc gagggttttc gcgcagccat tgccggaagc
     4921 ccggtgcgta ttgttgatat tgcgcttggt gataacgata ttgaaatcca gcgtaacctg
     4981 ttgcaggaga tgctggaacg ccacccagaa atcgacgtcg ttgccggaac agccattgcg
     5041 gcagaggcgg caatgggtga agggcgtaac ctgaaaacgc cgcttaccgt ggtgtcgttt
     5101 tatctttcac atcaggtgta tcgcgggctg aagcggggaa gagtgattat ggctgccagc
     5161 gatcaaatga tctggcaggg ggaactggcg gttgagcagg ccatcaggca attacagggg
     5221 caatcggtgt ctgataatgt cagcccaccg attttagttc tgacgccgaa aaatgccgac
     5281 cgtgaacata ttcgccgctc gctgtcacca gggggatttc gtccggtcta tttttatcag
     5341 cacacatcag cggctaagaa ataacattca ccatgttgcg tcaccagtaa atccgcgctg
     5401 agtttatgac gtaaacgacg aattaacaca tcgacggtgc gcaggtcagg gttttccacc
     5461 cgacgcgcag aaagcatacg tagcagacgt tcacggctga gaatttcgcc cggattcgtc
     5521 acaaatgcca ccaacatttc atactctgcg cgggtcagtt taatcggctc gccatcccgc
     5581 tccagcgtat ggcgcgacac attcaggcaa taaccggcaa agcgatagca gttgtcctga
     5641 gtgtgcggtt gagcttgtcg cgcgaggtcg attcgccaga gcagattttt cacccgtact
     5701 accagttcgc gcagttccag cggtttggtg acgtaatcgt ctgcgcccat ttccagccca
     5761 acaatacggt caatccgatc gctgcgtccg gtaaccagaa taatccccac cgttgagcgt
     5821 tctcgcaggg cgcgggttaa catcaggcca ttttcatcgg gtaagttgat atccagcaga
     5881 attaaatcta ccggctgagt ctgcataatt tcccgcagcc cggcaccgct cgctgtaacg
     5941 gaaacggtat acccctcctg agtgaagtaa gattgtaatc gcgcctgggt aaccggctca
     6001 tcttcaacaa taacaatgtg atgtggcatc agagggtttt actcattctg ttcatattct
     6061 gccgtaagcc gttcatcctg accagtgccg ctgttcatat ttgctcatta agatcgcttc
     6121 actaaaccat aattttacag gggctattat gcggaaactc tggaacgcgc tacgccgacc
     6181 cagtgctcgt tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct
     6241 gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg
     6301 ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc
     6361 cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa
     6421 gcgcaaactg gaagcgagca atgatatcta ccagaccttt attgctcact ccattgatac
     6481 acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa
     6541 agaaaacaac tcggcaacct gccgctcctg ccataactac gatgcgatgg atcatgcgaa
     6601 gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat
     6661 cgactgtcat aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca
     6721 gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat
     6781 taagccaatt tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc
     6841 ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac
     6901 ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc
     6961 ctcgattcgt ggtgatgtgc agcagcaggt aaaaacgctg gagaaaacca ccgttgccga
     7021 caccaatacc gagtggagca agttgcaggc cactgcgtgg atgaagaaag gtgatatggt
     7081 gaacgatatc aaaccgatct gggcttatgc ggattcgttg tacaacggca cctgtaacca
     7141 gtgccacggc gcaccggaaa tctctcactt tgatgctaac ggctggattg gcacgctcaa
     7201 cggcatgatt ggctttacca gtctcgataa acgtgaagaa cgcaccttgt tgaaatatct
     7261 gcaaatgaat gcgtctgaca tcgcaggtaa ggctcacggc gataagaagg aagaaaaata
     7321 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc
     7381 ttaacagtcg ccgggatgct ggggccgtca ttgttaacgc cgcgcagtgc gactgcggcg
     7441 caagcggcgg atgtcatctc gaaagagggc attcttaccg ggtcgcactg gggggctatc
     7501 cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact ggataaatat
     7561 ccgtcgaaaa tgattgccgg attgccggat tgccggattg ccggattgcc ggattgccgg
     7621 atcacgtaca caacgcggcg cgtattcgtt atccgatggt acgcgtggac tggctgcgta
     7681 agcgccatct cagcgatacc tcccagcgcg gtgataaccg ttttgtgcgc gtgagctggg
     7741 atgaagccct cgacatgttc tatgaagaac tggaacgcgt gcagaaaact cacgggccga
     7801 gtgccttgct gaccgccagt ggttggcaat cgacggggat gttccataac gcttcgggga
     7861 tgctggcgaa agctattgcc ttgcatggta atagcgttgg tacgggcgga gattactcta
     7921 ccggtgctgc gcaggtgatc ctgccgcgcg tagtcggttc gatggaagtg tatgaacagc
     7981 aaacctcctg gctgctggta ttgcagaaca gcaaaaccat tatgctgtgg ggctccgatt
     8041 tgctgaaaaa ccagcaagcg aactggtggt gcccggatca cgatgtttat gaatattacg
     8101 agcaactaaa agcgaaagtc gccgccggtg aaattgaggt catcagcatc gatccggttg
     8161 tcacatccac ccatgagtat ctggggcgcg agcatgtgaa gcacattgcg gttaacccgc
     8221 aaactgacgt gccgctgcaa ctggcgctgg cgtatacgct gtacagtgaa aacctgtacg
     8281 acaaaaactt tctcgctaac tactgtgtag gttttgagca gttcctgccg tatctgctgg
     8341 gtgagaaaga cggtcagccg aaagatgccg catgggctga aaaactgacc ggcattgatg
     8401 ccgaaaccat tcgtgggctg gcgcggcaga tggcggcgaa cagaacgcaa attattgctg
     8461 gctggtgcgt gcagcgtatg cagcacggtg aacagtgggc gtggatgatt gtggttctgg
     8521 cggcgatgct ggggcaaatt ggcctgccag gtggtggttt tggttttggc tggcactaca
     8581 acggcgcagg cacgccgggg cgtaaaggcg ttattctgag tggtttctcc ggctctacgt
     8641 cgattccgcc tgttcacgac aacagtgact acaaaggcta cagcagcacc attccgattg
     8701 cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat caactggaac ggtaaatcgg
     8761 taaaactgcc gccactgaaa atgtgtattt ttgccggaac taacccattc catcgccatc
     8821 agcagatcaa ccgcattatt taaggctggc gcaagctgga aacggttatc gccatagata
     8881 accagtggac ctcaacctgc cgctttgccg atatcgtact gcctgcgacc acgcagtttg
     8941 agcgtaacga tctcgaccag tacggcaacc actccaaccg tggcattatc gccatgaaac
     9001 aggtggtgcc gccgcagttc gaggcgcgca acgactttga tattttccgc gagctgtgcc
     9061 gtcgctttaa tcgcgaagaa gcctttaccg aagggctgga cgaaatgggc tggctgaaac
     9121 gcatctggca ggaaggtgta caacaaggca aaggacgcgg cgttcatctg ccagcgtttg
     9181 atgacttctg gaataacaaa gagtacgtcg agtttgacca tccgcagatg tttgttcgcc
     9241 accaggcatt ccgcgaagat ccggatctcg aaccgctggg cacgccgagt ggcctgattg
     9301 agatctactc gaaaaccatc gccgatatga actacgacga ttgtcagggg catccgatgt
     9361 ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc gcagaagtat ccgttgcatc
     9421 tgcaatctgt gcatccggat ttccgacttc actcgcagtt atgtgagtcg gaaactctgc
     9481 gtcagcaata tacggtagcg ggtaaagagc cagtattcat taacccgcag gatgccagcg
     9541 cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa cgctcgcggt caggtgttgg
     9601 caggggcagt agtttctgac cgctatgcac ccggcgtggc acgaattcac gaaggggcat
     9661 ggtacgatcc agataaaggc ggcgagcctg gtgcgctgtg caaatacggt aaccccaacg
     9721 tgctgaccat cgacatcggt acttcgcagc tggcgcaggc gaccagtgcg cacactacgc
     9781 tggtggaaat tgagaagtac aatggagcag tggagcaggt gacggcgttt aacggccctg
     9841 tggagatagt ggcgcagtgc gaatatgttc ccgcgtcgca ggtgaaatca tgaccacgct
     9901 gacagcacaa cagattgcct gtgtttacgc ctggctggcg cagttgttct cccgtgaact
     9961 ggacgatgaa caactgacgc aaatcgccag tgcgcagatg gcggaatggt tttcgttgct
    10021 gaaaagcgaa ccgccgctgg ctgcggcggt gaatgagctg gaaaactgta ttgccacgct
    10081 gacagtacgt gacgatgccc gtctggaact ggccgctgac ttttgcggcc tgtttttgat
    10141 gactgataag caggcggcgc tgccgtatgc atcggcctac aaacaggacg agcaagagat
    10201 taaacgcttg ttagttgagg ccgggatgga aaccagcggc aatttcaacg aaccggcaga
    10261 ccatctggcg atctatctcg agttgttgag tcatctgcat ttttcgctgg gagaggggac
    10321 cgttcctgcg cgaagaatcg acagtttgcg gcaaaaaaca ctgacggcgc tgtggcaatg
    10381 gttaccagag tttgctgcgc gttgccatca gtatgacagc tttggttttt acgcggcact
    10441 aagccagtta ttgctggtgt tagtggagtg cgaccaccaa aacaaataac gtcgtttatg
    10501 cgcctgaaaa gacgcgttta gcgtcgcatc aggcattatg gcgcagttgc cggatgcgtc
    10561 atcacatccg gcaaacacac atcacggatg agctacaaac cgggaaagcc gctggcgtag
    10621 caggcggttt tcctgcttca ggtgtgcaat atcatccatt aacgtcagcg ccaccgcgat
    10681 ccccggccag tccagagcca gttcatgacg caggcgtacc gcgcgttgca ccacaatggc
    10741 ggcatggtcg tcaaataccc aggttgtttc ctggatctca cgcggttcaa ccacccccaa
    10801 accgacaatt tcattcaact cctcttcaga gatgccggta tgcaggcaaa attcggtaat
    10861 agtaaaagtc accgtaacat tagccatcat gctttccccc aatctttacg tggatcaaaa
    10921 gacgactggg cgtctgccag ttgctgccac agcgcggcag tgttttcatc cggtttcggc
    10981 ggcatcacga ttttcagtac cgcatacaga tcgccggtct gttttttgct caccagacct
    11041 ttgcctttaa cgcgcaatcg ttgcccggcc tggctgcctg gcgggatagt cagcaaaatg
    11101 ctttctttca gtgttggaac ggtgacttta gcacccagcg ccgcttccca cgggctaacc
    11161 ggcaccacaa tttccagatc ctggccgaca atatcgaaca gcggatgtgg cgcaatatga
    11221 atcaccagcc acaaatcgcc atttggacca ccgttttcgc ccggcgtccc ctggcctttc
    11281 agacggatgc gttgaccatt gccgacgccc gccgggatct tcacattcag cgttttcggg
    11341 atttcctgtt cgatcatgcc aaaggcgtta taaaccggca ggttatagct gatggtacgc
    11401 ttatgctcag tcagcgtttc ttcgaggaat accgccactt cgatttcaat atcgtggccg
    11461 cgtgcggcgg ggcgttgacg gctctggcgg gcatgctgac cgaaaattga cgagaagata
    11521 tcgtcaaaat cttcggcatt aaaactctga ccgtcgccat gatggaactg acggttaaat
    11581 tgcggatcgt tgcgatgttg ccacatctga tcatactcag cgcgacgttg ttcatcactt
    11641 aacacttccc aggcttcagc gacctctttg aagcgggctt cggcatccgg ttctttgctg
    11701 acatcaggat ggtatttacg ggccagtcga cgataggcgg tcttgattgt cttgagatcg
    11761 tccgtcggtt tcacgcccat gatggcgtaa taatccttta attccatagc gttatctcgc
    11821 gtaaatcaac acaaattgaa ggaacccctg taaggtaact cctataagtg tagggtaatc
    11881 ctcaaaattt catatgccaa cacagaatat gttattgaaa tcatcgtgga gaggaggtcg
    11941 ccatcaatat gggttgctga acatatttta aacaggtgaa aaagggtgag cgatttttga
    12001 tagttgaacc aggcacttta agtttaacta gggcgtcatt atttattaaa tttt
//
LOCUS       DAJTSL010000124        11968 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTSL010000124 DAJTSL010000000
VERSION     DAJTSL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.124"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..414)
                     /locus_tag="OO433_003040"
     CDS             complement(<1..414)
                     /locus_tag="OO433_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6051434.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYVLTTQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVTKAGYIALAPDGLSSVG"
     gene            570..1610
                     /gene="gpr"
                     /locus_tag="OO433_003041"
     CDS             570..1610
                     /gene="gpr"
                     /locus_tag="OO433_003041"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6051435.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGVSRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1650..2144)
                     /locus_tag="OO433_003042"
     CDS             complement(1650..2144)
                     /locus_tag="OO433_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6051436.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2335..3219
                     /gene="yghA"
                     /locus_tag="OO433_003043"
     CDS             2335..3219
                     /gene="yghA"
                     /locus_tag="OO433_003043"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6051437.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3491..3916)
                     /gene="exbD"
                     /locus_tag="OO433_003044"
     CDS             complement(3491..3916)
                     /gene="exbD"
                     /locus_tag="OO433_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6051438.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDLVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3923..4657)
                     /gene="exbB"
                     /locus_tag="OO433_003045"
     CDS             complement(3923..4657)
                     /gene="exbB"
                     /locus_tag="OO433_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6051439.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLFNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            4909..6096
                     /gene="metC"
                     /locus_tag="OO433_003046"
     CDS             4909..6096
                     /gene="metC"
                     /locus_tag="OO433_003046"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6051440.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6236..6895
                     /locus_tag="OO433_003047"
     CDS             6236..6895
                     /locus_tag="OO433_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6051441.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGMLFVVIKKKYCN
                     A"
     gene            complement(6935..7834)
                     /gene="yqhC"
                     /locus_tag="OO433_003048"
     CDS             complement(6935..7834)
                     /gene="yqhC"
                     /locus_tag="OO433_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6051442.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            8028..9191
                     /gene="yqhD"
                     /locus_tag="OO433_003049"
     CDS             8028..9191
                     /gene="yqhD"
                     /locus_tag="OO433_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6051443.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAADNYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9296..10123
                     /gene="dkgA"
                     /locus_tag="OO433_003050"
     CDS             9296..10123
                     /gene="dkgA"
                     /locus_tag="OO433_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6051444.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            10323..11249
                     /locus_tag="OO433_003051"
     CDS             10323..11249
                     /locus_tag="OO433_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6051445.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11300..11557
                     /gene="yqhH"
                     /locus_tag="OO433_003052"
     CDS             11300..11557
                     /gene="yqhH"
                     /locus_tag="OO433_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6051446.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(11592..11900)
                     /locus_tag="OO433_003053"
     CDS             complement(11592..11900)
                     /locus_tag="OO433_003053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051447.1"
                     /translation="MDYFCRNESVIDTATQKIIIQPGCHFARSSAHITSQIETEFIGY
                     WYIVITGQWLVQTFYRFINRFFAQHVSAEATEQQNKKTGVPASHNHVLMFGFFLANSL
                     "
BASE COUNT         3096 a         3043 c         2982 g         2847 t
ORIGIN      
        1 tccaacggaa cttaagccgt caggtgccag ggcgatatac cccgccttcg tcactcgccg
       61 tgccacatct tcgatatacg gattcagtcc acgattctca tgcaccacca ccacggctgg
      121 cgttttgccg ctcatttttg cgggtttcac cagataaccc cgtacctcgc cgtgaccatt
      181 tggcgaagga tacgtgatgt actcagcaac aatctccggg tcggtaaact ctacctgagt
      241 cgtcagcaca taatttggct tgagcaaatc aaacaacgct aatgccgtca tcccgcccac
      301 cgcatacttc gccgcaaggt tgaggaactc acgtttcgaa attttcccgt gagcgtaata
      361 gtcgtagtaa tccaacaact cttgtgggaa atctttggcg gttaaacgcg gcatcgttgc
      421 attcctcaac tggtgttttt taagcaaagc ataagcgcgt gtttttgccc agtttttcgt
      481 cactctgtga gccagactac gggatacgcg ctggcgaatc gctaaactag aaacattgtt
      541 tcgaaattga acggtggaaa ggagaggtca tggtctggtt agcgaatccc gaacgttacg
      601 ggcagatgca gtaccgctat tgcggaaaaa gtggtttacg cctgcccgcg ttatcgctcg
      661 gtttatggca caatttcggt cacgttaacg cgctggaatc acagcgtgca atcctgcgta
      721 aagcgtttga tttaggcatt acgcactttg atttagccaa caattacggg ccgcctccag
      781 gaagcgcaga agagaacttt ggtcgcctgc tgcgggagga ttttgccgct tatcgcgatg
      841 aactgattat ctctaccaag gctggctacg atatgtggcc cggcccttac ggctctggcg
      901 gttcacgtaa atacctgctc gccagcctcg accaaagcct gaagcgtatg gggcttgagt
      961 atgtcgatat cttttactct catcgcgtcg atgaaaatac gccgatggaa gaaaccgcct
     1021 ctgcgctggc tcatgcggta caaagcggta aggcgctgta tgtcgggatc tcctcttact
     1081 cgccagagcg gacgcaaaaa atggtcgagt tgctgcacga gtggaaaatt ccgcttttaa
     1141 ttcatcaacc ttcgtacaat ttactgaacc gctgggtgga taaaagcggc ctgctggata
     1201 ccctgcaaaa taacggcgtg ggctgtattg cctttactcc tctggctcag ggattgctga
     1261 ccggaaaata tctcaacggc attccgcaag attcacggat gcatcgtgaa gggaataaag
     1321 ttcgtggtct gacgccgaaa atgcttaccg aagccaacct caacagcctg cgcttattga
     1381 atgaaatggc acagcagcgt ggacaatcaa tggcgcaaat ggcgttaagc tggttgctga
     1441 aagatgatcg cgtgacgtcg gtattgattg gtgtcagccg cgcggagcaa ctagaggaga
     1501 acgtgcaggc gctgaataat ctgacattta gcaccgagga gctggcgcag attgatcagc
     1561 atatcgccga tggcgagctg aatctgtggc aggcgtcttc cgataaatga cctgttaata
     1621 acgggcgcgc gaaccgcgcc cgaatcagat cagtgattat gacgagtcag tcggtcaaga
     1681 tagcccatca caaatgcaga gagcacaaac gtcagatgga taatgacgta ccacatcagt
     1741 ttgttatccg ggacattttt cgcatccata aagacgcgca gtaagtgaat ggaagaaatt
     1801 gccacaatcg acgctgctac tttgtttttc agcgacgttg cgtccatttt ccccagccag
     1861 ttcagcttct ctttgttctc ggagatatcc agctgcgaga cgaaattctc ataaccggaa
     1921 aacatcacca tcaccagcaa accgccaacc agcgtcatat ccaccagcga cagcaacacg
     1981 aggatcaaat ctgattccgc catcgagaag atattcggca gtacgtgaat aatctcctgg
     2041 aagaacttca gcgccagggc aactaacgcc agcgaaaggc caaagtacac gggggcaagc
     2101 agccagcgag aagcatacat tgcattttca agaaaacgtt ccatacattc ctgtcgtgta
     2161 gttaaaacgg gcaacagtat atcgcaattg agtaaacagc acgcaacagc aaatcaacat
     2221 atacgttaaa cctttgtcgc ataattctga ggtttaggga tcttcctggt tcccttatca
     2281 acgcatcgag agcgtctacc cttaaaagac gttgaacctg aagggagaaa aacaatgtct
     2341 catttaaaag acccgactac gcagtattac actggtgaat atcccaaaca gaaacaaccg
     2401 acgccaggca tccaggcgaa gatgacaccg gtaccggatt gcggcgagaa aacctatgtt
     2461 ggtagcggtc gcctgaaaga tcgtaaagca ctggtgacag ggggcgattc cggaataggt
     2521 cgcgctgccg ccatcgctta cgcgcgtgaa ggggctgacg tggcgatcag ttatcttccc
     2581 gtggaagaag aagacgctca ggatgtgaaa aagatcattg aagaatgcgg acgcaaagcc
     2641 gttctgctgc caggcgattt aagcgatgag aaattcgccc gttcgctggt tcacgaagcg
     2701 cacaaggcgt taggcgggct ggatattatg gcgctggtcg ccgggaaaca ggttgccatt
     2761 ccggatattg cagacctcac cagcgaacag tttcaaaaga cctttgccat taacgttttc
     2821 gcgctgttct ggctaaccca ggaagcgatc cccctgctac cgaaaggtgc aagtatcatc
     2881 accacttcgt caatccaggc ataccagcca agtccgcatt tactggacta tgcggctacg
     2941 aaggcggcga ttctgaacta cagccgtggc ctggcaaaac aggtcgcgga gaaaggtatt
     3001 cgggtgaata ttgtcgcgcc aggccctatc tggacggcac tgcaaatttc cggcggacaa
     3061 acgcaggata agatcccgca gtttggtcag caaacgccga tgaagcgtgc ggggcaaccg
     3121 gcggaactgg cccctgtata tgtttatctg gcaagtcagg agtcgagcta cgtcaccgca
     3181 gaagtgcacg gtgtgtgcgg cggcgagcat ttaggttaaa aaaatgcccg gttgtgaaaa
     3241 gcaaccgggc atcattgtga attcactctt acctgatgtg gcgaaaacgc cttatccgcc
     3301 tacaaacgca tgaagattca acatgttgca atagattttg taggcctgat aagcgtagcg
     3361 catcaggcat tgagcaccta atgccggatg cggcgtgaac gccttatccg gcctacaaac
     3421 gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta gcgcatcagg
     3481 caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc ttcaggtaac
     3541 ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg gttttatccg
     3601 ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac gccgtaatca
     3661 ttgtttcatc ggtgaccaga tcgttaccga taaacatcga gttgtctgcc ttcaccgaca
     3721 gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc aagttcacct
     3781 tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga accaacatca
     3841 cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg aggttttcgt
     3901 taagatgcat tgccatcgga tattatcctg cgcgtaattt ttgtgcgaca cgaaccggat
     3961 gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat acctgcgctg
     4021 caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg ttatagataa
     4081 cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct tctgcgatac
     4141 ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata aagctgttca
     4201 tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg atggttgcca
     4261 gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc tccagacgga
     4321 agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt tccagctcat
     4381 tctgcgcttc attgaacaaa tgcaggctta agcttttgct accaaaatca gcggcgatat
     4441 cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc tttaggcgac
     4501 gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaggtg actacggagg
     4561 ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga tacatacccc
     4621 agacggaaag gtctgtctgc attaaattat tacccactgt gtatctccag gacgcaagtc
     4681 acaaaatctg cgcataataa tatcaaaacg acgtcgaatt gatagtcatt ctcattacta
     4741 tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa aaaagtcacc
     4801 agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc gcccgcgaat
     4861 attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgacat ggcggacaaa
     4921 aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct tggcgcggta
     4981 aatagcgtga ttcagcgcgc ttcttcgctg gtatttgaga gtatggaagc caaaaagcac
     5041 gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac gttaacccat
     5101 ttttccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgc gctatttccc
     5161 tgcggggcgg cagcggttgc taattcgatt cttgcttttg tcgaacaggg cgatcatgtg
     5221 ttgatgacca acactgccta tgaaccgagt caggatttct gtagcaaaat cctcagcaaa
     5281 ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt taagcatctg
     5341 cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat ggaagtccac
     5401 gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat cattatgatc
     5461 gacaacacct gggcagccgg tgtgctgttt aaggcactgg attttggcat cgatgtttct
     5521 attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg cactgccgtg
     5581 tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg ccagatggtc
     5641 gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt gcgtttgcgt
     5701 caacatcatg aaagcagtct gaaagtggct gaatggctgg cagaacatcc gcaagtagcg
     5761 cgagttaacc accctgctct gcctggcagt aaaggacacg aattctggaa acgagacttt
     5821 acaggtagca gcgggctgtt ttcctttgtg cttaagaaaa aactcagtaa tgaagagctg
     5881 gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg cgggtatgaa
     5941 tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca aggcgagatc
     6001 gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga cgatctgatt
     6061 gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg gacaattttg
     6121 cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc gtcccggacg
     6181 aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa agtccatggc
     6241 tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc tggcggatcc
     6301 tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag aaaacggcct
     6361 gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg cattgattgc
     6421 ccagggggtt atggattttc tgcctacgat tgcgattctg accgccgcag caagtctggg
     6481 ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg tgaaaggctg
     6541 gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg accgccacgg
     6601 tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc tgccaaccat
     6661 ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt taagtggatt
     6721 gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc cgttcgttaa
     6781 acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct tgttaaccgc
     6841 tggcttgtta ggcatgctgt ttgtggtgat taaaaaaaaa tactgtaacg cctgacgatt
     6901 ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc gcccgcattc
     6961 ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg ctaaattgcg
     7021 atgcgctttc atagccgacg cgcatcgctg ctgcgctggc cttcatgccg tcatggatga
     7081 tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc gaggtgctgg
     7141 tgacagactt aaaattatgg tggaacgccg atacgctcat gttggcttct gccgccagtt
     7201 gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg cggctaatca
     7261 gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa ggtccggtca
     7321 gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca tccagtggtc
     7381 gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat aacgtggcgg
     7441 agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca atgtccatca
     7501 acagttcctg taactgcaaa atatcgacat tgagacgcaa ccctgccagc ggcacctctg
     7561 acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg tattcattgg
     7621 catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa aagagaatta
     7681 tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcacca tacaacaaac
     7741 gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta ttaactttat
     7801 ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta ggaataatgc
     7861 ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga aataggcaag
     7921 acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga aaaacatcag
     7981 gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg aacaacttta
     8041 atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt ttacgcgaac
     8101 aaattcctca cgatgctcgc gtattgatta cctacggtgg cggcagcgtg aaaaaaaccg
     8161 gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa tttggcggta
     8221 ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt cgcgaacaga
     8281 aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc aaatttatcg
     8341 ccgcagcgga taactatccg gaaaatatcg atccgtggca cattctgcaa acgggcggta
     8401 aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca accggttcag
     8461 aatccaacgc aggcgcggtg atctcccgta aaaccactgg cgacaagcag gcgttccatt
     8521 ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac accctgccgc
     8581 cgcgtcaggt ggctaacggc gtagtggacg cctttgtaca caccgtggaa cagtatgtta
     8641 ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg ctgacgctga
     8701 tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc gccaacgtca
     8761 tgtgggcggc gacgcaggcg ctgaacggtt tgattggcgc tggcgtaccg caggactggg
     8821 caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac gcgcaaacac
     8881 tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga taccaagcgc gctaagctgc
     8941 tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag cgtattgacg
     9001 ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc cacctctccg
     9061 actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa gagcacggca
     9121 tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt atatacgaag
     9181 ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt ccagacttaa
     9241 gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg aacgtatggc
     9301 taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg gactgggcgt
     9361 ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag aagtgggtta
     9421 tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca aagccctgaa
     9481 aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga acgacgacca
     9541 caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg attatatcga
     9601 cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag catggaaagg
     9661 catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca acttccagat
     9721 ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa accagatcga
     9781 acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac acaaaatcca
     9841 gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc agaaagtcat
     9901 tcgcgatctg gcagataaat acggcaaaac cccggcgcag attgttatcc gctggcatct
     9961 ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg ccgaaaactt
    10021 tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa aactcgatca
    10081 gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa attctcccgg
    10141 tggcggtaat gttccgctac cggacttttc agaaatcatt tattcccctc gcgtcccgcc
    10201 cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat gcagagccgg
    10261 aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga ggtgaaaaac
    10321 agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta cacgcgcagc
    10381 ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat aaatcagatg
    10441 ccagtacgcc ttatttcggt gaaaccggag agcgggcgat aacttcagcg cgtattcaac
    10501 aggcgcttac cctgaacgac aatcttacgc tgccgggcaa tattggctgg ctggattatg
    10561 atccggtttg tgattgtcag gattttggcg atctggtgct agaaagcgtt gcgataaccc
    10621 aaactgacgc cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa gatgataaag
    10681 aaaagaccac gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc attgacgata
    10741 ttgtcagcaa tcatggcagt gtcttacaag cagttaatag cgagaatgaa aaaacgctgg
    10801 ccgctttagc ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa ctctttgaac
    10861 atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac cgccaggcgg
    10921 ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa gatatgcaaa
    10981 tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg ctattttcac
    11041 gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt catgttcgtt
    11101 ttacgctgac caatggcaac aacgaagagc aagaactggt tttacagcgg cgcgaaggca
    11161 agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag cagattgagg
    11221 caaaaactgc cgccagatta aagcaatgag ctgaattaaa taacaattag ccggaacaat
    11281 aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg ttctggcaac
    11341 ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat cagctggcga gcaatgtgca
    11401 aacattaaat gccaaaatcg cccggcttga gcaggatatg aaagcactac gcccacaaat
    11461 ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgcac aggactattt
    11521 tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc ggcagcatgt
    11581 cagttgccgg gttataggct gtttgctaaa aaaaagccaa acataaggac atgattatgc
    11641 gacgctggca cacctgtttt tttattctgc tgttctgtgg cttcagcgga aacgtgttgt
    11701 gcaaagaatc gattaatgaa gcgatagaac gtttgtacca accattgacc agtaatgacg
    11761 atataccaat aaccgatgaa ttcagtctcg atttgcgaag taatatgagc gctactcctg
    11821 gcaaaatgac accctggctg aataataatc ttttgtgtcg ctgtgtcgat gacgctttcg
    11881 tttctgcaga aataatccaa agcacacacc taaatggcgg ccgaacgcat gtactgttgc
    11941 agctggttat caaacaaaat cgcacaga
//
LOCUS       DAJTSL010000125        11883 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTSL010000125 DAJTSL010000000
VERSION     DAJTSL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.125"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(39..353)
                     /locus_tag="OO433_003054"
                     /pseudo
     CDS             complement(39..353)
                     /locus_tag="OO433_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            complement(468..866)
                     /locus_tag="OO433_003055"
     CDS             complement(468..866)
                     /locus_tag="OO433_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HCR6051448.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQLESAPGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSSQANNDLLSAGMELLKGKLFR"
     gene            complement(1106..3520)
                     /gene="gyrB"
                     /locus_tag="OO433_003056"
     CDS             complement(1106..3520)
                     /gene="gyrB"
                     /locus_tag="OO433_003056"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6051449.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADLLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(3549..4622)
                     /gene="recF"
                     /locus_tag="OO433_003057"
     CDS             complement(3549..4622)
                     /gene="recF"
                     /locus_tag="OO433_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6051450.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWACFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLVEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(4622..5722)
                     /gene="dnaN"
                     /locus_tag="OO433_003058"
     CDS             complement(4622..5722)
                     /gene="dnaN"
                     /locus_tag="OO433_003058"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6051451.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(5727..7130)
                     /gene="dnaA"
                     /locus_tag="OO433_003059"
     CDS             complement(5727..7130)
                     /gene="dnaA"
                     /locus_tag="OO433_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6051452.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7427..7507
                     /locus_tag="OO433_003060"
     CDS             7427..7507
                     /locus_tag="OO433_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051453.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            7737..7877
                     /gene="rpmH"
                     /locus_tag="OO433_003061"
     CDS             7737..7877
                     /gene="rpmH"
                     /locus_tag="OO433_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6051454.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            7894..8253
                     /gene="rnpA"
                     /locus_tag="OO433_003062"
     CDS             7894..8253
                     /gene="rnpA"
                     /locus_tag="OO433_003062"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6051455.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8217..8474
                     /gene="yidD"
                     /locus_tag="OO433_003063"
     CDS             8217..8474
                     /gene="yidD"
                     /locus_tag="OO433_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6051456.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            8477..10123
                     /gene="yidC"
                     /locus_tag="OO433_003064"
     CDS             8477..10123
                     /gene="yidC"
                     /locus_tag="OO433_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6051457.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKTQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10229..11593
                     /gene="mnmE"
                     /locus_tag="OO433_003065"
     CDS             10229..11593
                     /gene="mnmE"
                     /locus_tag="OO433_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6051458.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            11836..>11883
                     /gene="tnaC"
                     /locus_tag="OO433_003066"
     CDS             11836..>11883
                     /gene="tnaC"
                     /locus_tag="OO433_003066"
                     /inference="COORDINATES: protein motif:HMM:NF019665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR6051459.1"
                     /translation="MNILHICVTSKWFNID"
BASE COUNT         2718 a         3253 c         3068 g         2844 t
ORIGIN      
        1 ggacaggggt atcgcttagt ttttcaattt cctatacgtg aaccgacttc gcgagagagt
       61 ttttccagga aacgatagtc gtcataactg agagcagttt gcgccacggt gctaccatca
      121 gcggccttct gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg ctgttccata
      181 tgcagctctt tcaggtagtt gtgaacgcct gcataagggc gacccgtcgt tagcacgaca
      241 ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga aatggtgtga
      301 tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc catgagttcc
      361 ccaggtagat tagttcctga cccatgctaa cgcgattccg ctcaaaaatc agtacaacac
      421 ccgagggaaa agggggatgc aacgcgggtt gcatgctccc catttgctta gcggaagagt
      481 ttccctttca gcagttccat acctgcggaa agcagatcgt tattggcttg tgaagacact
      541 tcaccttgcg gtgagagcgc atcaataatc ttcggcaatt gttctgccag taaactggaa
      601 gccgtactgg tatccacgcc aagtttttgc ccgagatcgg acaccgcatt tgtgccgggc
      661 gccgattcca gttgctcgcc gctaactgat tgattgctct gctgattact caaccaggtt
      721 gagagaatgg cccctaagcc gccactttgc agtttttcca gcagcacctg aatgccgccc
      781 tgctcctcaa cccaacttaa aatagcctga tatttccccg catcgccttt cagaaaggca
      841 ccgacaactt catcaaaaag ccccatgata atcacctgta aagcgttacg tgttgaccca
      901 aaaagtatag atctgcggga tgataattgc ggattgcaga aataaaaagg gcggagatga
      961 tctccgccct ttttttatag cttcttgccg gatgcggcgt taacgtctta cctggcctac
     1021 aaaatcatta aaattcaata cattgcaaga ttttcgtagg cctgataagc gtagcgcatc
     1081 aggcacgctc gcatggttaa cgccattaaa tatcaatatt cgccgctttc agggcgttct
     1141 cttcaataaa cgcacgacgc ggttctacgg cgtcgcccat cagcgtggtg aacaacaggt
     1201 cagcggcaat cgcatcctta acggtaacgc gcagcatacg acgactttcc gggtccatgg
     1261 tggtttccca cagctgttcc gggttcatct cgcccagacc tttataacgc tggatggaga
     1321 gaccgcgacg agactctttc accagccagt ccagcgcctg ctcgaagctg gctaccggct
     1381 gacgacgctc gccacgttcg ataaatgcat cttcttccag caagccacgc agtttctcac
     1441 ccagcgtgca gatacgacga tattcgccac cagtgataaa ttcgtgatcc agcggatagt
     1501 cagtatccac accgtgggta cgcacgcgaa caatcggctc gaacaggttt tgttcggcat
     1561 tggtgtggac atcaaacttc cactggctgc cgtgctgttc tttgtcgttc agttcgctga
     1621 ccagcgcgtt cacccagcgg gtaacggtct gctcatcaga gaggtcggct tccgtcagcg
     1681 tcggctgata gataagctct ttcagcattg ctttcggata acgacgctcc atgcggttga
     1741 tcattttctg cgtcgcgttg tactcagaca ccagtttctc taacgcttcg ccagccaatg
     1801 ccggtgcgct ggcgttggtg tgcagcgttg cgccgtccag cgcgatagag atctggtact
     1861 gatccatcgc ttcgtcgtct ttaatgtact gttcctgctt gcctttcttc actttgtaca
     1921 gcggcggctg agcgatgtag acgtgaccgc gttcaacgat ttccggcatc tgacgataga
     1981 agaaggtcaa cagcagcgta cgaatgtgcg agccgtcgac gtccgcatcg gtcatgatga
     2041 tgatgctgtg ataacgcagt ttgtccgggt tgtactcgtc acgaccgata ccacagccaa
     2101 gcgcggtgat aagcgtcgcc acttcctgag aagagagcat cttatcgaag cgcgctttct
     2161 caacgttgag gattttaccc ttcagcggca gaatcgcctg gttcttgcgg ttacgcccct
     2221 gcttcgcaga gccgcccgcg gagtcccctt ccaccaggta cagttcggaa agcgccggat
     2281 cgcgttcctg gcagtctgcc agtttgcccg gcaggcccgc taagtcgagc gcacctttac
     2341 ggcgggtcat ttcacgcgcg cgacgcgccg cttcacgggc acgggcagca tcgataattt
     2401 tgccaaccac gattttcgcg tcggttgggt tttccagcag gtattctgcc agcagttcgt
     2461 tcatctgctg ttcaaccgcc gatttcacct cagaagaaac cagtttgtct ttggtctggg
     2521 aggagaattt cgggtccggc acttttacgg aaacgaccgc aatcaggcct tcacgcgcat
     2581 cgtcaccggt ggcgctgact ttggcttttt tgctgtagcc ttctttgtcc atgtaggcgt
     2641 tcagggtacg ggtcatcgcc gcacggaagc ctgccaggtg agtaccgccg tcacgctgcg
     2701 gaatgttgtt ggtaaagcag tagatgtttt cctggaagcc atcgttccac tgcaacgcca
     2761 cttcgacgcc aataccgtct ttttcggtgg agaagtagaa gatattcgga tggatcggcg
     2821 ttttgttctt gttcagatat tcaacgaacg ccttgatgcc gccttcatag tggaagtggt
     2881 cttctttgcc gtcacgcttg tcgcgcagac gaatggaaac gccggagttg aggaacgaca
     2941 actcacgcag acgtttcgcc agaatttcat attcgaactc ggtcacattg gtgaaggttt
     3001 cgaggctggg ccagaaacgc accatggtgc cggttttttc agtctcgccg gtaaccgcca
     3061 gcggagcctg cggtacaccg tgttcgtaga tctgacggtg aattttaccc tcgcgctgga
     3121 taaccagctc cagtttttgc gacagggcgt ttactaccga aacaccaacg ccgtgcagac
     3181 cgccggacac tttataggag ttatcgtcaa atttaccgcc tgcgtgcaga acggtcatga
     3241 tcacttccgc cgccgatacg ccctcttccg ggtgaatacc ggtcggaatg ccgcgcccgt
     3301 catcctgtac agagacagag ttatcggcgt gaatggtgac gataatttct ttacagtgac
     3361 ccgcgagcgc ttcgtcgata gcgttatcta ccacctcgaa taccatgtgg tgcagaccgg
     3421 tgccgtcatc cgtgtcgccg atatacatac ccgggcgctt acgcaccgca tccagccctt
     3481 tcaggacttt gatactggag gagtcataag aattcgacat caacgtttct cgctcattta
     3541 tacttgggtt aatccgttat tttacccttt tccacggtaa acatcttcga attttcgtcc
     3601 gacatgtcta taacgtgttc agcactgatc gcgctgacaa agacctgtga ttgcgtcgct
     3661 tttaagcggc tggcaagcag cccgcgacgc tcatcatcaa gctcagaggc aaaatcatct
     3721 atcaggtaga gacaccgccg cccgctttca cgggtgagga actctccttg cgccagacgt
     3781 aaggcgcaca tcaacagctt aagctgccca cgcgataagg tatcttccac cggcgcaccg
     3841 tcggcgcgaa tgcgtaagtc cgctttatgc gggccatgcg cggtgtaggt tagctggcga
     3901 tcgcgttcaa aattacgttc cagcacctca gcatattctg tctctttctc ccagccgcgc
     3961 tggaaagaga aagtcagaga aaactcaggg agaaattgct tacaagtatc ggccatatca
     4021 gccgcgatac cggcgctata ctccgcgcgc caggtgctga tttgctccac cagcgggatc
     4081 agctctttat cccacgggcg tagctgttcg taacgtgtca cctggcgcag cgccgcattg
     4141 cgctgcttga gcaatcgctt gagattgctc caggcggtga aaaatccggg ttcgttgtga
     4201 aagcatgccc agtcgagaaa tgctcttctg tatttggggc cgccgttgag taaagtaaac
     4261 ccttctggcg ttatcagctg catcggcatc aggtgcgcca gctccgcgac tttatgcccg
     4321 tcagtaccgt cgatgcggac tttgctgtcg ccttgtttgt ctttggttaa gccaatcgcc
     4381 gtctcgcgct cttcgccctg taatcgccca tggagaacaa atgcctcctg ctcatggcga
     4441 atgacgcgac caatctgcaa actgcgaaac gcccgaccat ggccgagcgt atagatggct
     4501 tccagcacac tggttttgcc actgccgttg gcacctacca gaaagttaaa gccgggagat
     4561 aaggcgagat ccgcggtttc gatattgcgg aaatcgcgga tcaacaagcg ggtgagggac
     4621 attacagtct cattggcatg acaacataag ccgcgctctg gctggccgca tcttcaatct
     4681 gcacgctgga aaccgaatcg gtcagcatca tgcggacgtt ttcgcatttc agcgcgttca
     4741 gaacatccag cacatagctg acgttgaagc cgatttccat ctccgcaccg ctataggtaa
     4801 cgtcgaggat ctcttccgct tcttcctgtt ccgggttgtt ggcagtgatt ttcagctggt
     4861 tttcgctgac ataaagacgt acgccgcgga atttctcgtt agagagaatt gccgcacggg
     4921 caaacgcctg cttgagcaga tcgcagccag cttccagatg tttgtccggg ttcttcggta
     4981 gaacgcggcg gtaatccggg aagcgaccat ccaccagttt ggaggtgaag ataaagtcgc
     5041 caacgtgggc acgaatgtta ttgctgccaa tctgcacgcg cagcggattg tcgccgccgt
     5101 cgagcatacg catcagttca atcacgcctt tacgcggcac gatcaccgaa tggcttggca
     5161 aagattgacc aattggcatt gaacagactg ccagacggtg accatcggtc gctacggtgc
     5221 gcagttcttc gccttcggtt tcaaacagca taccgtttaa gtaatagcga acatcctgat
     5281 gcgccatcga aaactgggtc gcttcaatca gacgcttcat cgtcgcctgc ggcagggtaa
     5341 attcgacttc gctctgccag tcatcgaggt tcgggaaatc cgccgctggc agggtagaca
     5401 gcgaaaaacg gctacgcccg gagcgcacca gcatccgttc accttccagc tgcacggcaa
     5461 tttccgcgcc ttcaggcaga ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg
     5521 ttgctcctgg ctcgtgtggc tgaaccagcg caacacgtgc caccatttcc atctcgagat
     5581 cagtaccggt cagcgacaac gtaccgtcag caacctgtaa cagcagatta ccgagaatcg
     5641 gtagcgtagg acgaccacct aacggaccgc tcacctgttg tagcggtttt aataaatgct
     5701 cacgttctac ggtaaatttc ataggtttac gatgacaatg ttctgattaa atttgaaaaa
     5761 tcttctttga tatcgtggct ctcttcacgc aactgctcga tcttacggca ggcatgaagc
     5821 accgtcgtgt ggtcacgacc accaaacgca tcgccaatct ccggcagact gtggttagtc
     5881 agctctttcg ccagcgccat cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc
     5941 ttggaaagga gatccgcgac tttgatcttg tagtactccg ccaccgtctt ctgaatattg
     6001 tcgatggtga ccagtttttc ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag
     6061 tcgatggtga tcgcccgtcc ggtaaagttg gcattggcaa tgacgcggtt cagcgcccct
     6121 tccagctcac gtacgttaga tcgtagacgc ttggcgataa agaacgccac ttcgcccggc
     6181 aaacgaatgt cgttttcgtc ggcctttttc atcaggatcg ccacacgggt ttccagctct
     6241 ggcggttcga tcgccacagt cagtccccaa ccgaagcggg atttcaaacg atcctcaacg
     6301 ccgttgatct ctttcggata gcgatccgag gtgagaatga tctgttgatt accttccagc
     6361 agggcgttga aggtatggaa aaactcttcc tgagatcgtt ctttattagc aaaaaactga
     6421 atatcgtcga tcagcagtgc atctacggaa cggtagtagc gtttaaactc ttcgatcgcg
     6481 ttgttttgca gggctttaac catgtcctga acaaagcgct cggagtgcat ataaaccact
     6541 ttggcattcg gcttgcgcgc cataatgccg ttacccaccg catgcagtag gtgagtttta
     6601 cccagacccg tgccgccata aaggaacaac gggttatagg caccgccagg gttatccgcc
     6661 acctggcgag ccgccgcgcg cgccagttgg ttagatttac cttcaacgaa gttatcaaac
     6721 gtgtgtttga cgtttacgtt agaacgatag gtcggttctg ccggggccgg gacgttatcc
     6781 caacctgagc gcgtagaagg cgcagcacgt tgcggctgcg tttgcgccac ctgtgcaggg
     6841 gccgcgacgt tgctcgtcac tgccgcttgt ggcgtttgcg tcaccggttt ggtgccgact
     6901 tcaaaacgca gctgtggggc atccgctccg cagaaactgg ttagcagtcc attgatatta
     6961 ttaaggtact tgtcccgtac ccaatcgagg acaaaacggt ttggcgcgta cagggccagc
     7021 gtgttatcgc tcagttccgc ctgcaatggg cgtatccaca tactgaattc tgtggctggt
     7081 aactcatcct gcaatcgggc aagacactgc tgccaaagcg aaagtgacac ggcggactcc
     7141 actcgaacaa aagtcgataa tgactaaggc tgaaacattc atgattgttg acgtacgtcg
     7201 aaaagaccct gcttgagggt gacgcacgaa ccgctgtctg cggttatatg cccgatcaag
     7261 atcctgcaaa acgatcggga ccgcggatca tagcctaaac tgcgcaagag atcttctgtt
     7321 tctcacagat tttcccgatt tatccacagg actttccaga actcgctaag tgtaaacgat
     7381 cctgccgcga ggcgggcacg atttacgcca catattgaaa aatttaatga ccatagacaa
     7441 aaattggctt aatcgatcta ataaagatcc aggacgatcc ttgcgcttta cccatcagcc
     7501 cgtataatcc tccacccggc gcgccatgct ggtttccact ggtgtgaggt cgtacatttt
     7561 ccctgcgaaa aggtgcggaa aagcgcggta aataaggaaa gagaattgac tccggagtgt
     7621 acaattatta caatccggcc tctttaatca cccatggctt cggtgtccat cgtttcattt
     7681 ttcggcggat atccaataaa gccattgaat ttattcaagt ttaggtagaa atcgccatga
     7741 aacgcacttt tcaaccgtct gtactgaagc gcaaccgttc tcacggcttc cgtgctcgta
     7801 tggctactaa aaatggtcgt caggttctgg cacgtcgtcg tgctaaaggc cgcgctcgtc
     7861 tgaccgtttc taagtaatca agctaacccc tgagtggtta agctcgcatt tcccagggag
     7921 ttacgcttgt taactcccag tcaattcaca ttcgtcttcc agcagccaca acgggctggc
     7981 acgccgcaaa ttaccattct cggccgcctg aattcgctgg ggcatccccg tatcggtctt
     8041 acagtcgcca agaaaaacgt tcgacgcgcc catgaacgca atcggattaa acgtctgacg
     8101 cgtgaaagct tccgtctgcg ccaacatgaa ctcccggcta tggatttcgt ggtggtggcg
     8161 aaaaaagggg ttgccgacct cgataaccgt gctctctcgg aagcgttgga aaaattatgg
     8221 cgccgccact gtcgcctggc tcgcgggtcc tgatagccct cattcgggtc tatcaacgcc
     8281 tgattagtcc gctactcggg ccgcattgtc gtttcactcc aacctgttca agctacggaa
     8341 ttgaggcatt gcgcaggttt ggagtgataa aaggcagttg gttgacggtg aaacgcgtat
     8401 taaaatgcca ccctttacac cctggtggtg acgatcccgt cccgcccgga ccatttgata
     8461 ccagagaaca ctaacgatgg attcgcaacg caatctttta gtcatcgctt tgctgttcgt
     8521 gtctttcatg atctggcaag cctgggagca ggataaaaac ccgcaacctc aggcccaaca
     8581 gaccacgcag acaacgacca ccgcagcggg tagcgccgcc gaccagggcg taccggccag
     8641 tggccagggg aaactgatct cggttaagac cgacgtgctt gatctgacca tcaacacccg
     8701 tggtggtgat gttgagcaag ctctgctgcc tgcttacccg aaagagctga actctaccca
     8761 gccgttccag ctgctggaaa cttcaccgca gtttatttat caggcacaga gcggtctgac
     8821 cggtcgtgat ggcccggata acccggctaa cggcccgcgt ccgctgtata acgttgaaaa
     8881 agacgcttat gtgctggctg aaggtcaaaa cgaactgcag gtgccgatga cgtataccga
     8941 cgcggcaggc aacacgttta ccaaaacgtt tgtcctgaaa cgtggtgatt acgctgtcaa
     9001 cgtcaactac aacgtgcaga acgctggcga gaaaccgctg gaaatctcca cctttggtca
     9061 gttgaagcaa tccatcactc tgccaccgca tctcgatacc ggaagcagca acttcgcact
     9121 gcacaccttc cgcggcgcgg cgtactccac gcctgacgag aagtacgaga aatacaagtt
     9181 cgataccatt gccgataacg aaaacctgaa catctcttcg aaaggtggtt gggtggcaat
     9241 gctgcaacag tatttcgcga cggcgtggat cccgcataac gacggtacca acaacttcta
     9301 taccgctaat ctgggtaacg gcatcgccgc tatcggctat aaatctcagc cggtactggt
     9361 tcagcctggt cagactggcg cgatgaacag caccctgtgg gttggcccgg aaatccagga
     9421 caaaatggca gctgttgctc cgcacctgga tctgaccgtt gattacggtt ggttgtggtt
     9481 catctctcag ccgctgttca aactgctgaa atggatccat agctttgtgg gtaactgggg
     9541 cttctccatt atcatcatca cctttatcgt tcgtggcatc atgtacccgc tgaccaaaac
     9601 gcagtacacc tccatggcga agatgcgtat gctgcagccg aagattcagg caatgcgtga
     9661 gcgtctgggc gatgacaaac agcgtatcag ccaggaaatg atggcgctgt acaaagctga
     9721 gaaggttaac ccgctgggcg gctgcttccc gctgctgatc cagatgccaa tcttcctggc
     9781 gttgtactac atgctgatgg gttccgttga actgcgtcag gcaccgtttg cactgtggat
     9841 ccacgacctg tcggcacagg acccgtacta catcctgccg atcctgatgg gcgtaacgat
     9901 gttcttcatt cagaagatgt cgccgaccac agtgaccgac ccgatgcagc agaagatcat
     9961 gacctttatg ccggtcatct tcaccgtgtt cttcctgtgg ttcccgtcag gtctggtgct
    10021 gtactatatc gtcagcaacc tggtaaccat tattcagcag cagctgattt accgtggtct
    10081 ggaaaaacgt ggcctgcata gccgcgagaa gaaaaaatcc tgattcggtg agttttcgct
    10141 aaaataaggg cggtcagttg accgcctttt ttcttttcgt agggcggata agcaccgcgc
    10201 atccgccaca caaagcaaca ggaacatcat gagcgataat gacactatcg tagcccaggc
    10261 cacgcctccg ggacgtggcg gcgttggcat cctgcgcatc tccggcttca aagcccgtga
    10321 agttgccgaa accgtgctgg gtaaactgcc taagccgcgc tacgccgatt atcttccgtt
    10381 taaagacgcc gacggcagcg tactcgatca ggggattgcg ctatggttcc ctggcccgaa
    10441 ctcgttcacc ggcgaagatg tgctggaact gcaaggtcat ggcggtccgg tgatcctcga
    10501 cctgctgtta aaacgtattc tgaccattcc cggcctgcgg attgctcgcc ctggtgagtt
    10561 ttccgaacgc gcgtttctta acgataaact tgacttagcc caggccgagg cgattgccga
    10621 tcttatcgac gccagttcgg aacaggcggc ccgttcggca cttaactcgc tgcaaggcgc
    10681 attctccgca cgggttaatc atctggtaga agccctcacc cacttgcgca tttacgtcga
    10741 agcggcaatt gatttccccg atgaagagat cgatttcctc tccgacggaa aaattgaagc
    10801 ccagctcaat gacgttattg ccgatcttga tgcagtgcgt gctgaagcac gtcagggtag
    10861 tttgttgcgc gaagggatga aagtggtgat tgccggacgt cctaacgccg gtaaatcgag
    10921 cctgttaaac gcgctggcgg ggcgtgaagc ggcaatcgta accgatatcg ccggaactac
    10981 gcgtgacgtg ctgcgtgagc atatccacat tgacggaatg ccgctgcata tcatcgatac
    11041 cgccgggcta cgtgaagcca gtgacgaagt agaacgtatt ggtatcgagc gcgcgtggca
    11101 ggaaattgaa caggccgacc gcgtgctgtt tatggtcgat ggcaccacaa cagacgccgt
    11161 ggatccggca gagatctggc cggaatttat tgcccgtctg ccagcgaaac tgccgatcac
    11221 cgtggtgcgc aataaagccg atatcaccgg cgaaacgctg ggaatgagtg aagtgaacgg
    11281 tcacgcgtta attcgtctct cggcaaggac tggtgaaggc gtggacgtgc tgcgtaacca
    11341 tctcaaacag agcatgggct ttgacaccaa catggaaggc ggcttcctgg cgcgtcgtcg
    11401 ccacctacag gcgctggaac aggcagcgga acatctacaa cagggcaaag cgcaactgtt
    11461 gggagcctgg gcaggtgaac tgctggcgga agagttgcgt ctggcacagc agaacttaag
    11521 cgaaatcacc ggggaattta cttcagacga cctgctgggg cggattttct ccagcttctg
    11581 tattggtaag taaccgcgct tacgaagccg tattctgact gtcagatgcg gcttcgcttc
    11641 attgttagca ctcctgttat tcctcaaccc tttttttaaa cattaaaatt cttacgtaat
    11701 ttataatctt aaaaaaagca tttaatattg ctccccgaac gattgtgatt cgattcacat
    11761 ttaaacaatt ttagaataga caaaattctt gagtattata ctgtagcctc gtgtctttcg
    11821 aggaataagt gcattatgaa tatcttacat atatgtgtga cctcaaaatg gttcaatatc
    11881 gac
//
LOCUS       DAJTSL010000126        11689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTSL010000126 DAJTSL010000000
VERSION     DAJTSL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.126"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            63..218
                     /locus_tag="OO433_003067"
     CDS             63..218
                     /locus_tag="OO433_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051460.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
     gene            418..702
                     /locus_tag="OO433_003068"
                     /pseudo
     CDS             418..702
                     /locus_tag="OO433_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
     gene            complement(740..2473)
                     /gene="ggt"
                     /locus_tag="OO433_003069"
     CDS             complement(740..2473)
                     /gene="ggt"
                     /locus_tag="OO433_003069"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6051461.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAVAAPPVSYGVEEDIFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAQPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            2593..3033
                     /locus_tag="OO433_003070"
     CDS             2593..3033
                     /locus_tag="OO433_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6051462.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(3020..3763)
                     /gene="ugpQ"
                     /locus_tag="OO433_003071"
     CDS             complement(3020..3763)
                     /gene="ugpQ"
                     /locus_tag="OO433_003071"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6051463.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVVGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(3760..4830)
                     /gene="ugpC"
                     /locus_tag="OO433_003072"
     CDS             complement(3760..4830)
                     /gene="ugpC"
                     /locus_tag="OO433_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR6051464.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIVLSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(4832..5677)
                     /gene="ugpE"
                     /locus_tag="OO433_003073"
     CDS             complement(4832..5677)
                     /gene="ugpE"
                     /locus_tag="OO433_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6051465.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATTTF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(5674..5877)
                     /locus_tag="OO433_003074"
                     /pseudo
     CDS             complement(5674..5877)
                     /locus_tag="OO433_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="with UgpEC is involved in the uptake of
                     glycerol-3-phosphate; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter permease"
     gene            complement(5807..6478)
                     /locus_tag="OO433_003075"
     CDS             complement(5807..6478)
                     /locus_tag="OO433_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR6051466.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENRHLPGDRRRHVRRAGSGH
                     HDADL"
     gene            complement(6875..7588)
                     /gene="livF"
                     /locus_tag="OO433_003076"
     CDS             complement(6875..7588)
                     /gene="livF"
                     /locus_tag="OO433_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6051467.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRAASGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(7590..8357)
                     /gene="livG"
                     /locus_tag="OO433_003077"
     CDS             complement(7590..8357)
                     /gene="livG"
                     /locus_tag="OO433_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6051468.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(8354..9631)
                     /gene="livM"
                     /locus_tag="OO433_003078"
     CDS             complement(8354..9631)
                     /gene="livM"
                     /locus_tag="OO433_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6051469.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(9628..10554)
                     /gene="livH"
                     /locus_tag="OO433_003079"
     CDS             complement(9628..10554)
                     /gene="livH"
                     /locus_tag="OO433_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6051470.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(10602..>11689)
                     /gene="livK"
                     /locus_tag="OO433_003080"
     CDS             complement(10602..>11689)
                     /gene="livK"
                     /locus_tag="OO433_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6051471.1"
                     /translation="IAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDMEFNGARQA
                     IKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCSSSTQPASD
                     IYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVKPQRIAIIH
                     DKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENIDFVYYGGYY
                     PEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYDQDPANQGI
                     VDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGANTVIGPLN
                     WDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         2811 a         3353 c         2915 g         2610 t
ORIGIN      
        1 atgccatctt tttagagatt accagcagaa aattccttgt tcgaaattta gcagtgcaag
       61 ctatggaaaa acttacaacg gaactacact ccttaagtga gatggaccga agacatgtag
      121 tctctatttt agccgaaata gctaatggct atgatgattt taatgacatg ttgatttatt
      181 tagaatttta cccgaatcat aagattctga tatcttaaat aagaaaatcc cgttcgacgt
      241 tctgaaaaag aaatcataaa atattagcag atacttattt cgtgactata tcttatccga
      301 tgaacttttt tcaaaaccag catttaataa tatctttgac gaagatgaac gcgtctttgc
      361 tcaatgtatc acacaggaat aataatataa cactcaacaa taatatttat tataattatg
      421 attacttatc acgacgcatt cgcgaaagcg aaccattacc ttgatgatgc agatctcccg
      481 gtcgtcatta ctctacatgg acgctttagc tagggctggt atttctgttt cgaagcacga
      541 gaatttctcg aaactggaga tgaggccgcg cgcttagctg gtaacgcacc ttttattatt
      601 gataaagaca gtgctgaaat tcattctctg ggaacggcaa agccgctgga agaatatcta
      661 caggattacg aaataaaaaa ggctaccttc ggcttgccct gacaaaatag ccctcttccc
      721 acgaagaggg ccgctaacct tagtaccccg ccgttaaatc atccaccgag cgcgggtcgg
      781 atgcgccgta caactcaccg tccggcccaa ccataatgct ttgtgtactg cccatcgcct
      841 ctttcagcgc cactttctga ccttttgctt ccagcagctt gagcgtatcc gggctaaacc
      901 ctttttcgac acgcagctcg tccggcaacc actgatggtg gaaacgcggc gcattggtcg
      961 cttcggcgac gttcatgcca taatcgatgc tattcaccac catttgcagc actgtagtga
     1021 tgatccggct cccgccaggg ctaccggtga ccagccaggt tttaccgtct ttcaccacaa
     1081 tcgttggtga cattgacgac agcggacgtt tattcggccc gacggcgttg gcatcaccgc
     1141 ccaccagccc gtaaacgttc ggtacgcccg gtttagcgga gaaatcatcc atctgattat
     1201 taagcagaat accgctctcg cccgcgacaa tgcccgtacc gaaggtggtg ttcagcgtat
     1261 aggtcaccgc caccgcgtta ccatctttat ccaccactga gtaatgggta gtttgattac
     1321 tctcataagg cgcaagcttg ccggggcgaa tttcgctgga tggctgcgct ttattgatat
     1381 caatttgatc ggcaatagat ttggcatagg ctttattggt cagcgcctgc cacggtactt
     1441 tgacaaaatc cgggtcgcca agatattccg agcggtcggc gtaggcgtat ttctccgctt
     1501 ctgccatgat ttgcatcgca tcggcgctgc caaagccgta tttcttcata tcgaagtttt
     1561 ccagaatatt gaggatttgt acgatatgga tcccgccgga ggatggcggt ggcatggagt
     1621 aaacctgata cccgcgataa tcgccactta tcggtgtgcg ttcgaccgct ttatacgccg
     1681 ctaaatcttc tttagtgatc aagccaccgt ttttctgcat ctcctgggca atctgttccg
     1741 ctatcgtgcc tttatagaat tcgtccgggc cgttttcggc aatcatctcc aggctctttg
     1801 ccaggttcgc ctgcaccagc ttgtcgccct ttttcagcgg ttcgccctct ttccagaaga
     1861 tagctttgct gttttcgtga ttcggcaaca cttcgctacc gtaggttttg agatcgtctg
     1921 ccaaagcatc gttaacgata aaaccatcgc gtgccagttt gaacgcgggc tgcaccactt
     1981 tgttcaacgg catagtgccg tacttatcca acgccagcga gaaaccagcg accgtaccgg
     2041 gcgtgccgga agccagatgc gaagtgagtg attttttgct gtccgggttg ccctggtcat
     2101 caaggaacat atcgcgggtc gctttagcgg gtgccatctc gcggaaatcg atagcggtgg
     2161 tattgccatt tttcgagcgg atcagcataa aaccaccacc gcccagattc cctgcctgcg
     2221 gatgcgttac cgccagcgcg tagcccaccg ccacggcggc atcaacggca ttcccgccct
     2281 cctttagaat atctaccccc acttgggtgg cagtggcatc gacagacgcc accatcccct
     2341 gtttcgcgcg taccggatgg aagatatctt cctccacgcc gtaagaaaca ggaggcgcgg
     2401 cgacggcgct aaaacaactt cctgagagca gagcagcaat ggctacccgg cgtaaaaacg
     2461 tcggttttat catcgttatt ctccagagat taaggggcaa ccccagctaa gcctggtata
     2521 taactctgaa ttaatcatcg ttttgtcggg aagcgagtaa acttaaagga tatcttcaga
     2581 ggaggatacg cgatgaaacg acttctgatt cttacggcac tcctgccgtt tgtcggcttt
     2641 gcacagccca ttaatactct gaacaaccct aaccagccgg ggtatcagat acccagccag
     2701 cagcggatgc aaacccagat gcaaactcag caaatccagc aaaaagggat gctgaatcag
     2761 caactgaaaa cgcaaactca gttgcaacag cagcatttag aaaaccagat aaacaataat
     2821 tctcagcggg tgttgcagtc acagccgggg gagcgaaatc ccgcccggca gcaaatgctg
     2881 cccaacacca acggcgggat gttaaacagc aaccgtaatc cggatagttc gttgaatcag
     2941 cagcatatgc tgccggagag gagaaacggc gacatgctga atcagcccag cacgccgcag
     3001 cctgacattc cgttgaaaac tattgggccg taaagttcgg accaatcacg tcaatcgcat
     3061 cggtacagat gcaatccacg ccccagcgca gcaactctgc cgcgcgctgg ggtttattga
     3121 cggtataaac cagaatccgt aatccggcgt ctttcaactg catcactcgc gctttatcga
     3181 gtaacttatg attgagatga atagagacgc agcccagccg cgcggtcagt tcgcgccagt
     3241 cgtcgcgcca ctcatcaagc aataaaccac gcggcagttc cggtgccgcc agctgtgcag
     3301 cttctaaagc atcaatctca aacgatgaca gcagcggcgg cgtcataccg gcccacagtt
     3361 ggcgtgccgc cagcgccacc attttgcccg ttaatggtcc agtgccagtg gtgggtttga
     3421 tttcgatatt cgccatcatc ccatgttcgc gacagcgttc cgccacctgc gaaagcaacg
     3481 gcagcggctc acctttaaac gctttgctgt accaactgcc cgcatccacg cgcagtaaat
     3541 cctgccagtt cagttcaccc acgacgcccc agccgttgct ggtgcgttcg agattgtcgt
     3601 catggagcag gaagatctcg ccatctttcg ataacttcgc gtcaaattcg atcatcttat
     3661 gaccgtattt tgccccgacg tcgattgccg ccagggtgtt ttccggggcc agcttaccgc
     3721 cgccacgatg agcgacgatg cggggataag gccagttact catactcgtt gtcctgtttc
     3781 accatcaaaa agatgcagct gattttccgc cagatgcagc cacagcgtgc tgcctgccgt
     3841 cgggcgctcc tgatgcgcca gtcgcaccac cagcttctgc tcgccccagc gtccgtgcgc
     3901 caggttatct gcgccgagga tctccagcgt gtccatcacc agcggtacgc cgccttctgc
     3961 ctgcgagctt agcacaatat gttccgggcg aatgccgaga gtcattttac gcccggcata
     4021 ctgacggtag ccaccgttta gcggcagctc aataccgccg tccagttcga aatgcgtgcc
     4081 ttcgttattc acgcggcctg tcagcaggtt catcgccgga ctgccgataa aactcgctac
     4141 aaacaggctg gcgggctttt cgtagacttc aactggtgtg ccaatctgtt cggcaacgcc
     4201 gccgttcatc accattactc gctgggcgag cgtcatcgct tcaacctgat cgtgagtaac
     4261 gtagagtgaa gtcgttttca ggcgacggtg caactgttgc agttcaagac gcatctgcac
     4321 gcgcagcttg gcatcgaggt tagagagcgg ctcatcaaac aggaacaccg ccggatcgcg
     4381 cacaatcgcg cggcccatcg ctacacgctg acgctgaccg ccggatagct cacgcgggcg
     4441 acgtttgagc agaccgtcca gctccagaat gcgcgccgct tctttaacgc gctcggcaat
     4501 ttgctgcttg cccatgccgc gaattttaag cccccacgcc atgttttctt cgacgctcat
     4561 atgcggataa agcgcgtagt tctggaacac catcgcaatc ccgcgatctt tcggctccat
     4621 ttcagtcacg cgctggtcgt tgatccagat atcgccttcg gtcacccgct ccagcccggc
     4681 aaccatacgc agcagcgtcg atttcccgca gccagacggc ccgaccatca cgataaattc
     4741 gccatcagcc acatcaaggg tcagcggttt aatgacctgg gttttgccat cccagctttt
     4801 ggttactgcc tgtaatttca gtcctgccat cttatttctc actatcgacc aggccgcgca
     4861 cgaaggcacg ctgcatcact aaaacaatca ctactggtgg gatcagggtt aacaacatcg
     4921 ccgccatcac tgagttccat tccgtggtgc cttcgccagt ggcgatcatc cctttgatcc
     4981 ctgccacggt ggtgccgaga tccacgtcgg taataatcaa caacggccac aaatactggt
     5041 tccagccgta aataaaggtg atcacaaaca gcgccgccag attagttttg gagagcggaa
     5101 aaacgatgtc gcaaaagaag cgcattggcg atgcgccgtc gatccgcgcc gcttccacca
     5161 gttcatccgg cagcgtcata aagaactggc gaaacagaaa cgtggtggtc gccgaggcca
     5221 tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt caggttggcg atgacttcca
     5281 ccgtcgggaa gatacgtact tcaaccggca gcatcagggt gataaaaatc atccagaaga
     5341 agaggttacg tagcggaaaa cgaaaccaga caatggcaaa tgccgagagc atcgagacgg
     5401 taattttgcc gagcgtaatg ctgaacgcca tcacaaagct gttaagcaac atccgccaga
     5461 acggcgcgct attcgtacct accccgttaa cccagatgtt gtggatattt tccagcagat
     5521 gtgtgccggg gatgagcgtc atcggcgcgg catagacggc ctgtttatcc agcgtcgccg
     5581 cgacaaacgc cacgtacagc gggaagagaa tcaccgcgat cccgaggatc agcatggtat
     5641 ggctgaatat cgtcagccac ggacggttct caatcattgg taacgcacct tgctttcaac
     5701 atagcggaac tgcaccaccg tcagcacgat gacgaggaac atcaacacca ccgactgtgc
     5761 ggcagacgaa gccagatcca gtccggtaaa accttcgcgg tagatcttat aaatcagcgt
     5821 cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg atcaccggga aggtgtcgat
     5881 tttccagttg gatccagccc tgccagccgc tggcgtagcc gcacttcatg ccggaggctt
     5941 tcagtttcgc ggcatagtcc gccagatcct gccaggtttt cggcggctgt tccgggtcta
     6001 accccgcttt cttgaaggcg tctttgttgt aatagagaac gggagtcgag ctgttgaatg
     6061 gctgggagag taagtggccc gttttgctgt cggagtagta accggaaacc gtcggcacaa
     6121 actgcgactc atcgaattga atccccgcct ctttaaacac gtcatatacc ggtttaatgg
     6181 ctttcgatgc catcatggtg gcggtgccaa cttcataaac ctgcaaaata gccggagcgt
     6241 tgccggtacg aaatgcggca atccccgcgc ttaaattctg ttcgtagttg cctttatagg
     6301 tcggtacaat tttgtaatcc ggattttcgg cgttaaaacg ttgggccaga gaatccacct
     6361 ctttacccag ttccccttcc atagaatgcc agaacggaat ggtcgtcact gcctgtgcat
     6421 tccccattaa cgccagtccg agcgccagtg ctgaagctgt ataatgtaac ggtttcatcg
     6481 tttatctctc ttgttgtacc gaatgcgcga attcacgcgt tttatgctcg cggggtaaca
     6541 tgacatgctc gaattacaga aaaataactt ttttgttaca tttgtaagat agtaaggtgt
     6601 cagaaagatg acaaggcggt gacggcgtgg gtgagggaaa atgggagatg gggcacggat
     6661 aagcggaaaa atagaaggtc tgaatcaaac tctacagatt actcatcgtt tcatgccgga
     6721 tgcggcgtaa acgccttatc cggcctacaa gatcgtgcaa attcaatata ttgcaactca
     6781 cccagtaggc ctgataagcg cagcgcatca ggcaatttta catttgtcac ctgtctcaaa
     6841 ggagtctttt gactccttat caatcaacgt gttattaccc gcctaaatac gcacttctca
     6901 ctgcttcgtt cgccagcagc gcatcgccgg tatcggagag caccacatgg ccgttttcca
     6961 gcacgtagcc gcgatccgcc agctttagcg cctggttggc gttctgctcg acgaggaaga
     7021 tagtcatccc ctgctcgcgc agctgctcga tggtgtcgaa aatttgctgg atgataatcg
     7081 gcgcaagacc gagcgatggc tcatcaagta gcagcaaacg cgggttgctc atcagcgcgc
     7141 gaccaatcgc cagcatttgt tgctcaccgc cggacatggt gcctgcccgc tgaacacggc
     7201 gctcatgcag acgtggaaac agttcataca cccactttat gcgctcctgg aactggtcgc
     7261 gttcggcaaa aaagccgccc atcgccaggt tctcttccac cgtcatccgc gagaagacgc
     7321 gacgcccttc cgggacaatc gctaccgctt cgcgcatgat tttcgccgtc tgccagtcgg
     7381 taatgtcttt atcatcaaac acaattcgcc cgctggcggc gcgcggatcg ccgcataacg
     7441 tgccgagcaa ggtggttttc cccgccccgt tcgcgccaat cagcgtgaca atctcgccct
     7501 gattgatatg caggctcacc tcatgcagtg cctggatttt gccgtagtgg gcgctgactt
     7561 tgtcaaagga caacatgact ttttccatct tatgcctcac ctaaataggc acggatcacg
     7621 tccgggttat tacggatttg ctccggcgta ccgtttgcca gtggcgtccc ctgattgacc
     7681 acgtaaattc ggtccgaaat tcccatcacc agcttcatat cgtgttcaat caacaagata
     7741 gtggtgttgt gatgattgcg cagttcggca atcagctcat ccagctcttt cgtctctttc
     7801 gggttaagac ctgccgcagg ttcgtcgagc attaaaatct ccggctgcgt caccatgcag
     7861 cgggcaatct caagacggcg ctggtcacca taggccaggt tactcgcctg acggttggcg
     7921 tgttccagca aaccaatgcg ctcaagccag gtcgcggcgc gatcgagcgc ttcgctctgg
     7981 gcgcgacgga aggatggcgt tttcaacagg ccagagaaca gcccggtttt cagttgctga
     8041 tgctgcgcca ccagcaggtt ttcaatcacc gtcatttcac ggaacagacg cacatgctgg
     8101 aaggtacgca ccacgcccat gcgggcaatt tgctgccccg gtaaaccttc cagatgctga
     8161 tcgcgcagta aaatggtgcc tccagtgggt ttgtagaatc cggtcaggca gttaaagacc
     8221 gtggtttttc cggcaccgtt aggaccgatt aacgagacga tctcctgcgg gtacagttca
     8281 agattgacgt tgttcaccgc cagcaggccg ccgaagcgca tcatcaggcc gttcacagat
     8341 aataatggct gactcatgcc tgctctcctt tcgctgcgcc gtttttcagc ttcagttgcg
     8401 ggcgcgtcat gggcagcaag ccctgcggac gccagatcat catcagcacc atcaaaccac
     8461 cgagcattaa catgctgtat tcgttgaaat cacgcatcaa ctcgcgcgac accaccagca
     8521 aaattgccgc cagaatcacc gcaaactgcg atcccatacc gccgagcacc actatcgcca
     8581 gtacaaacgc cgattcagca aaggtgaagg attccgggct gacaaagccc tggcgcgcag
     8641 caaacagcgt tccggcaaaa ccggcaaacg cggcactgat ggtaaaagcg gtcagcttga
     8701 tacgacgcgg gcttaagccc agcgaacggc aggcgatctc atcttcacgc aacgcttccc
     8761 acgcacgccc cagcggcatc cgcagcaggc ggttaatgac aaacaggctt agcaccacca
     8821 gcagcaacgc caccagatac aggaagatga cgcgatcgga gggatcgtat ttcaggccaa
     8881 agaaattact gaacgtgtcc caaccgcctt cacgggcggt acggctgaac tcaaggccga
     8941 agaaagtcgg tttcgggatc tgactgattc cgttcgggcc gccggtaatt tcggtgttat
     9001 tgagcagcaa tatgcgcaca atttcgccga agccgagggt aacgatcgcc agataatcac
     9061 cgcgcagacg cagcaccgga aagccgagca ggaagcccgc cgccgctgcc attaacccgg
     9121 caatcggcag acaagtccag aagcccaagc cgtaatagtg attgagcagc gcaaaggtgt
     9181 aagcgccgat ggcgtaaaaa ccgccgtacc ccagcaccag cagaccagaa agaccaacaa
     9241 ccacattcag cccgagaccg aggataatgt agatcatggt cagagtggcg atatccaccg
     9301 tcccacgtga caccataaac ggccacgcca ccgcaagcac caacagcgcc acgaggaaca
     9361 gtttctgctt caccgtggag ccatcaatcg cgggcagaat aaacttcggt ccggaaacgc
     9421 tttttagccc tttctggaaa gccggtcgca aaagctggaa gaaaaagacc accgccgtgc
     9481 cgataaacac ccactgccaa cggatgtctg aagccgtgtc gaccaccagt ttggtgccat
     9541 ccagctccag ttgcacgccc ataaagacgc ccgccaggac aaaaaacatc gcggcagaga
     9601 gcagcgccat tgcaatatgc atcggtttca tactttctct acctccgggc gacccagaat
     9661 accggtcggc atcaccagca gcaccagaat cagcagggca aatgaaacca catccttata
     9721 ttccgtactc agataggcag aagagagcgc ctccgcaatc cccaggatca gcccaccaat
     9781 catcgcccct ggaatgctgc caatcccacc gagcaccgca gcggtaaagg ctttcatccc
     9841 ggccataaag ccgatatagg ggttaatgac gccgtagaac tgaccgagca gtacgccagc
     9901 cactgccgcc atcgccgcgc caataacaaa ggtcaaagca atcacccggt cggtgttgat
     9961 gccaagcagg ctcgccattt tcagatcttc cgcgcaggca cggcaggcgc gccccatacg
    10021 ggaataacga atgaaaatcg tcagcgccag catggcgagg aaagtaacaa tccagatcac
    10081 tgcctgcatg gtggtaatag aggcagagaa gttttcgcta tgccccacca cccactgacc
    10141 gttaaacaga ctgggcagcg ccacgtcacg cgaaccttca gtcaggctaa cgtagttttg
    10201 caggaagatg gacatgccga ttgccgagat gagcgcaatc aggcgcttag agttacgcac
    10261 cgggcgatag gctacccgtt cgatactcca gccgtaggcg ctggcaatga cgattgcgcc
    10321 gacgaatccc gccgccacca gcagccagcc ggtatcaatg cccatcatca tcagcgcggc
    10381 gatgatcata aatgagacgt agctgccaat catataaacc tcgccgtggg cgaagttgat
    10441 catgccgata atgccgtaaa ccatggtgta gccgatggct atcagcgcgt aggtactgcc
    10501 cagcgtgacg ccgttaaaca tctgctgcaa gaaatacaaa aactgctcag acataaggta
    10561 acctttctaa acccgcccgc attttacggg cggtgggatg atcacttggc tgccgtggat
    10621 gaaccgtcgg cgtgccactg gaagacacca aaatcaaatc ccttaagatc gcctttttca
    10681 tcccagttca gcggcccaat cacggtgttt gcaccgttag cttttaaatc tttcaccagc
    10741 gccagcggct catcgctgcc agtacgctca agggcagtcg ccagagattg caccgccgcg
    10801 taggtgatcc agacataagg cccggacgga tctttcttgt ctgctttcag cgcatcaacg
    10861 atgccctggt ttgccggatc ctggtcatag cgttttggca tagtgaccaa catgccttcg
    10921 gcagcatcac cggcaatatt cgacaatgat gcgttaccca caccttccgg ccccataaac
    10981 tgagttttca ggccaacgga acgggcctgg cgcagcatct gccccatttc cgggtagtaa
    11041 ccgccgtagt aaacgaagtc gatgttttct tttttcaggc gggcgatcag cgcggagaaa
    11101 tctttctccc cggcggtaat accgtcgaag aagacgacgt tggcgttagc cgctttcagc
    11161 ccgtcctgca ccgaacgcgc cagcccttcg ccatactgtt gtttgtcgtg aatgatggcg
    11221 atgcgctggg gcttcaccgt ctcaagaatg tattttgccg ccgttggccc ctgggaagag
    11281 tccagcccgg cagtacgcat aatgtgttga taaccgcgtt gggtcagctc cgggttggtc
    11341 gctcccggtg agatcatcag aataccttcg tcttcgtaga tatctgatgc aggttgggta
    11401 gaagaagaac acagatgacc aataacgtat ttaatgccgt cattaacgat tttgttggcg
    11461 accgcaacgg cttgtttcgg gtcgcaggcg tcgtcatatt ccacgccaac cagcttgtcg
    11521 cccttaattc cccctttggc attaatgtct ttaatcgcct gacgcgcgcc gttaaattcc
    11581 atatcgcccc actgggcaat cgggccggac atcgcgccga caacggcgac tttaatatcg
    11641 tcagccatag cggtgtgtga aattgccagt gcaatcatcc ctgcgatga
//
LOCUS       DAJTSL010000127        11628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTSL010000127 DAJTSL010000000
VERSION     DAJTSL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.127"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(51..1037)
                     /locus_tag="OO433_003081"
                     /pseudo
     CDS             complement(51..1037)
                     /locus_tag="OO433_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
     gene            complement(1246..1905)
                     /locus_tag="OO433_003082"
     CDS             complement(1246..1905)
                     /locus_tag="OO433_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6051472.1"
                     /translation="MANWLNQLQSLLGQSSSSISSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(2232..2588)
                     /locus_tag="OO433_003083"
     CDS             complement(2232..2588)
                     /locus_tag="OO433_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6051473.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(2655..2949)
                     /gene="yebG"
                     /locus_tag="OO433_003084"
                     /pseudo
     CDS             complement(2655..2949)
                     /gene="yebG"
                     /locus_tag="OO433_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3083..4261
                     /gene="purT"
                     /locus_tag="OO433_003085"
     CDS             3083..4261
                     /gene="purT"
                     /locus_tag="OO433_003085"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6051474.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCSDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(4317..4958)
                     /gene="kdgA"
                     /locus_tag="OO433_003086"
                     /gene_synonym="eda"
     CDS             complement(4317..4958)
                     /gene="kdgA"
                     /locus_tag="OO433_003086"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6051475.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4995..6806)
                     /gene="edd"
                     /locus_tag="OO433_003087"
     CDS             complement(4995..6806)
                     /gene="edd"
                     /locus_tag="OO433_003087"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6051476.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQSPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRRFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(7041..8516)
                     /gene="zwf"
                     /locus_tag="OO433_003088"
     CDS             complement(7041..8516)
                     /gene="zwf"
                     /locus_tag="OO433_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6051477.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            8853..9722
                     /gene="hexR"
                     /locus_tag="OO433_003089"
                     /gene_synonym="yebK"
     CDS             8853..9722
                     /gene="hexR"
                     /locus_tag="OO433_003089"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6051478.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9850..11292
                     /gene="pyk"
                     /locus_tag="OO433_003090"
     CDS             9850..11292
                     /gene="pyk"
                     /locus_tag="OO433_003090"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6051479.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(11352..11570)
                     /locus_tag="OO433_003091"
     CDS             complement(11352..11570)
                     /locus_tag="OO433_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051480.1"
                     /translation="MEYQADYYITINDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGM
                     RRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         2819 a         2994 c         2905 g         2910 t
ORIGIN      
        1 ctgctcaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc aaccagccag
       61 tcggtaaaca gcgtaaaccc ttccagcatg atgttttcgc gtggcggaat taactcttcc
      121 cactgttgct catcacgcag gcgggtacgg tataagccaa agtttttgcc gtggcggttg
      181 gaacgcagat aaaaccgatg ctggtagtga tcaaggctgt attcgtgatc tttgcggcgc
      241 ggcagaaaaa caaacggctc ggcatcggcc atttccgcgt ccagcaggcg aacttcactg
      301 gtggtggcgc tggccaaatg aatgactaca tagtgcttcg aggtcgtttt atgcaggctg
      361 acgtaatagg tatcgtcttt ttcttcgtag atcagtttat cttgcgatgc tggcgtaccg
      421 atggcgtgac gccagacctg ataaggcagc agcgtcaccg gatgcttgcg aacatagtag
      481 aaagtccagg agtcatttgc ccagacaaag ctgggttcaa cgttatccag cagttccggg
      541 taccagttac cagtttccag attacgaaaa cgaatgccgt actggcgtcg ggaaagaaaa
      601 tcttctgcca gcgccataat ggtgttatcg ggcgtaatcg ccattccgcc catcgaataa
      661 aactcactat gagctgcgcg cttattggca tcgagcaatg tttcccactc atcccactct
      721 tcactgaatg ccgattgacg ctggtagata gcatattcac agcctggttc ataaatatgc
      781 cgatagcggt agccattttt gatgtagggc gcagaaactt ctcgttgcgg aatgcggtcg
      841 atgatttcct ttaagatgcg atcctgcaag gcttgttgtg aggccatcac ccgatgaccg
      901 taactatttt cttgttgcag gtagtccagg acttctggct gagaacgcgt atcgtcccgc
      961 agccagtagt aattatcgat gcgcgtatcg ccatgaagcg tcatggcgtg gggaatgcgg
     1021 gcggcttttg gtagcatgtt attgttcttt ctggttgaaa catcttataa gggtggcaaa
     1081 actcaccggg gatgcaagcg aaacagggga gtcattgctt agatgatgac aggtaatggc
     1141 gcggatatcg aatgttatgc agacagagaa aatcagcctg ttcaaatggc tgtgcgattc
     1201 tggatagccc gaaatagtca acttcaggct atccagagag cggaattatt ccgccaaagt
     1261 gcgtttttgc tgttcgagat cgcgttcaat gccgtcacga acatcctggg ggattttcag
     1321 cgcgtcaccc agtgcattca ggtaactgcg ttccataaaa tggtcaatat caatagccgc
     1381 gcaactcagg aaatagattt ccagcgcctc ttcttcattg cggaccccgg tagccaagcg
     1441 ttgtggatcc agcggttgtt cgattgcctg ctcaatgagt acacgcccct gctcttccac
     1501 gccggcttca cgcaattgct ggtcgattgc cgcacgttct ttggcatcaa tatgaccatc
     1561 acttttagcg gcaaagacca gcgcaaggat caaacgttct gtacgctcat ccagcggcgt
     1621 actttgcgcg ccaaactgcg gttcgtcctg atgcgccgcg cgaattttat ctttgtattt
     1681 attccacagc accgtacccg ctaccgctcc gccgccaacc agtaacgcgt tggtgccata
     1741 tttcgtaaga agtttacgtg ctgatttatt tgcgaccagc agcccagcca gtccgcctaa
     1801 tgcgcctggc actaacagtt tgcccaatcc ctgatccgca gacgaggaga tagaagaact
     1861 gctttgccca agcagggatt gcagttgatt taaccagtta gccatatttg ctcctcaata
     1921 accattttta gtgcctgtag catagcggat gaggatgtca gaaaatgtat atcgagacaa
     1981 aagatgcgca aataagcggg gcggtaggcc gttattcaaa gaaattcgcg ccaggtaagg
     2041 tatttactgt atatcaggtg ataagggtat tttaggtgaa agtagtttgt cgttttgagt
     2101 ggcattgttt gatgctgatt gcccgatgcg acgctgttgc gtcttctcag gcctacgatt
     2161 gtggtgcgtt ctggggcgga caaggcactc gcgccgcatc cgccaacggt caatcgaatt
     2221 atcagaagcc attaacgccg ctgatattcc gccattcccg ctttgcagtc cacgctgacc
     2281 tggtaatgaa tatcggcact tttaccacgc acggttagcg gtactgacca tttatcatct
     2341 ttaccctgaa tgtcctgcaa actgacccaa gccacgggat cggcctgacc gacaattttt
     2401 tgatcatctg cccaacgcgc cacgcgattt tgttgataat cacgttttac gctcgcggca
     2461 attccggcag catccagacc ttcacacttt gggaaagtga ccgacttgct ggtttcatta
     2521 ttggcagcga aaactgatgc gcaggcagaa accaacaaca gccctaaaaa cgcccctctt
     2581 tttttcatgt ttttctccat agcacaatga ttcaggagaa agcatggtac aaattgtcag
     2641 gagcgcaagt tgcttcaggc cgcgtgtgag gcatcttcct cttcggattc agcaccgacg
     2701 acctgtgggt ggggacggta atttgccggt tttcagaatg gtgctcagca catctttttg
     2761 ttctgccagc catagcgaaa gggcttcacg ttgctcgtct tccatttgca ctggagaatt
     2821 agtcagccag gtgtcaagca gatccgccgt atcaagcatt ttgtcataag catcggcttc
     2881 ctttttgctg gtaaacgaca ttttctcttc gccctcacga atgactacgt atttaacttc
     2941 aaccgccatt tgcagcctct cataataact gtgattttat acagtgtatt tcttttcggt
     3001 tgagaaatca aaatcaacaa taaagacaca cgcaaacgtt ttcgtttata ctgcgcgcgg
     3061 aattaatcag gggatattcg ttatgacgtt attaggcact gcgctgcgtc cggcagcaac
     3121 tcgcgtgatg ttattaggct ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg
     3181 tctcggcgta gaggtgattg ccgtcgatcg ctatgccgac gcaccagcca tgcatgtcgc
     3241 gcatcgctcc catgtcatta atatgcttga tggtgatgca ttacgccgtg tggttgaact
     3301 ggaaaaacca cattatatcg tgccggagat cgaagctatt gccaccgata tgctgatcca
     3361 acttgaagag gaaggactga atgttgtccc ctgcgctcgc gcaacgaaat taacgatgaa
     3421 tcgcgagggt atccgtcgcc tggcggcaga agagctgcag ctgcccactt ccacttatcg
     3481 ttttgccgat agcgaaagcc ttttccgcga ggcggttgct gacattggct atccctgcat
     3541 tgtaaaaccg gtgatgagct cttccggcaa ggggcagacg tttattcgtt ctgcagagca
     3601 acttgctcag gcatggaagt acgctcagca aggcggtcgc gccggagcgg gccgcgtaat
     3661 tgttgaaggc gtcgttaagt ttgacttcga aattaccttg ctaaccgtca gcgcggtgga
     3721 tggcgtccat ttctgtgcac cggtaggtca tcgccaggaa gatggcgact accgtgaatc
     3781 ctggcaacca cagcaaatga gcccgcttgc ccttgaacgt gcgcaggaga ttgcccgtaa
     3841 agtggtgctg gcactgggcg gttatggtct gtttggtgtc gagctgtttg tctgtagtga
     3901 tgaggtgatt ttcagtgagg tctcccctcg tccacatgat accgggatgg tgacgttaat
     3961 ttctcaagat ctttcagagt ttgccctgca tgtacgtgcc ttcctcggac ttccggttgg
     4021 cgggatccgt cagtatggtc ctgcagcttc tgccgttatt ctgccacaac tgaccagtca
     4081 gaatgtcacg tttgataatg tgcagaatgc cgtaggcgca gatttgcaga ttcgtttatt
     4141 tggtaagccg gaaattgatg gcagccgtcg tctgggtgtt gcgctggcga ccgctgagag
     4201 tgttgttgac gccattgaac gcgcgaagca cgccgccgga caggtaaaag tacagggtta
     4261 aacccggcaa aaaaacgcta caaaaatgcc cgatcgtaga tcgggcgttt tgacttttac
     4321 agcttagcgc cttctacagc ttcacgcgcc agcttagtaa tgcggtcgta atcgcccgct
     4381 tccagcgcat ctgccggaac cagccaggaa ccaccgatgc acagcacgct tttcagcgcc
     4441 aggtagtcac ggtagttagc cggagaaata ccacccgtcg ggcagaaacg gacctgggag
     4501 aacggacccg cgatcgcctg cagggctttc acgccgccgt tagcttcagc cgggaagaat
     4561 ttgaactctt tcaaaccgta gtccataccc agcatcagtt cggaaacagt gctgatcccc
     4621 ggaattagag gaatagtccc ttcggtagca gctttcagca gcggctcggt cagacccggg
     4681 ctaattgcga actgtgcacc cgcttcagtg acttctgcca gctgctgtgg attcagcacc
     4741 gtaccggcac ccacaatcgc ttcaggcact tctttggcga tagcacggat agcgtcaact
     4801 gcacactcgg tacgcagagt cacttccaga acgcgcaccc caccagcaac caacgctttt
     4861 gccatcggca ctgcgtgttc cagttttttt accacgataa ccggtacaac cgggccggtg
     4921 gtcaggattg attctgcact tgttttccag tttttcatca gagttttctc tcgcctgatt
     4981 acaaatttgt cgtcttaaaa agtgatacag gttgcgccct gttcggcacc ggacagtttt
     5041 tcacgcaagg cgctgaataa ttcacgtcct gtccccacgc gtgacgcgct caggtcagga
     5101 atgtgcggtt cgcgagcagc cagttccgct tcgtctacca gcagcgtcag ttcgcctgtc
     5161 tgtccattca cacgaatgat gtccccgtcg cgcacttttg ccagcagccc gccatcgtag
     5221 gcttctggtg ttacgtggat agctgacggc actttacctg aagcgccgga gagtcgtcca
     5281 tcggtaacta acgcaatttt gaaacgccgg tccaataata caccaagtgg cggcatgagt
     5341 ttatgtaatt ctggcattcc gttcgctttt ggcccctgat gacggacaac aacgacacaa
     5401 tcgcggtcca gcaaacccgc ttcaaaggcc ggcataacgt catgctggct ttcaaaaaca
     5461 accgctggcg cttcaatcac ctggttctca accggcacgg cagaggtttt cataaccgca
     5521 cggcccaggt taccgcttaa cacttttgtc ccaccatgat gagagaaagg ttgttcgaag
     5581 gaagcgatca cattgctgtc gagtgatttt tccgcccctt cccgccagtc cagttcacca
     5641 ttattcagcc atggttcaag ggtataacga gacagaccaa agcctgccac cgtattgaca
     5701 tcttcatgca gcaggcctgc tttgagcagt tcacgcacca gaaccggtac gccacctgcc
     5761 gcctggaagt ggttaatatc ggccggaccg ttcgggtaga gacgtgccat cagcggtaca
     5821 acatcagaaa ggtcagagaa gtcatcccag ttaatctgaa taccggccgc gcgcgccatc
     5881 gccaccaggt gcatggtgtg gttagtggaa ccaccggtcg ccagcagtgc aacgataccg
     5941 ttcaccacca ctttctcatc gatcatctta ccgatcggca tccattcatt accattaccg
     6001 gtcatgcgtg taacctgacg cgcagcggca gcggtcaaag catcgcgcag cggagaatcc
     6061 ggatgaacaa aagaagagcc tggcgactgc atccccataa attccaccac catctggttg
     6121 gtgttggcag taccgtagaa agtacatgtt cccggcgcat ggtaagacgc ggcttctgac
     6181 tccagtaagg ccatgcggtc cactttacct tcggcataaa gctggcgaat acgcactttt
     6241 tctttatttg gcaaaccgct tgccatcggt ccagacggca caaacaccgc aggcaaatga
     6301 ccaaacgaca gggctgccat cgtcagaccc gggacaatct tgtcgcacac accgaggaac
     6361 agagcaccat caaacatgtt atgggacagc cccaccgccg cagacatcgc tatcacttcg
     6421 cggcttagca gcgacaattc cattccatcc tgcccctggg tgacaccatc acacatcgcc
     6481 ggaacaccgc ccgcaacctg accaaccgca ttcgcttcat gcagggcttt acgaatgatt
     6541 tctggatagt gttcataagg ctggtgcgcg gagagcatgt cgttatagga ggtgatgatg
     6601 gcgatattgt tacgcaacat gcttttcaaa gaggctttgt cttctggctg gcaggcagcg
     6661 aaaccgtgtg ccaggttacc gcatgccaac tgcgaacgat gaacggtcga cgttttcgct
     6721 tgttctatcc gggcgagata agcagagcga gtctcgcgcg aacgttcaat gattcgattt
     6781 gttacgcgta acaattgtgg attcataaag gctcctgaaa ttgagttgtc agagcaggat
     6841 gattcacaac gcgtttcatt cagaggattt atgagtgaaa cgcctgtaac cggagctcat
     6901 agggcaaaaa cgtttcagtc agtgtaataa aaaaagcctc gtgggtgaat ccgcacgagg
     6961 cctgaaagtg taaaaattgt tctacaatct gtgcaagatc atgttaccgg taaaataacc
     7021 ataaaggata agcgcagata ttactcaaac tcattccagg aacgaccatc acgggtaatc
     7081 atcgccaccg aggcaacggg tccccaggtt ccggcctgat acggtttcgg cgcatcattg
     7141 tccatcgccc acgcctcagt aatggagtct acccatttcc aggcttcttc cacttcatca
     7201 cgacgtacaa acagtgcctg aataccacgc atggtttcca gcagcagacg ttcataggca
     7261 tccgccagat gcgtctgatt aaaggtttct gaatagctca gatccagctt ggtgatttgc
     7321 aggttatgtt tgtggtcaag gccaggaact ttattcagta cctggatatc cacgccttca
     7381 tcaggttgca gacggatagt cagtttattc tgcggcagat cctgccacga ctctttaaac
     7441 agattcagtt caggtgtttt gaaatagacc acgacttcag aacatttggt cggcagacgt
     7501 ttaccagtac gcaggtagaa tggcacaccg gcccagcgcc agttatcaat gtcgacgcgg
     7561 atcgccacga aggtttctgt attgctgctc ttgttcgcac cctcttcttc cagatatccc
     7621 ggcacttttt tgccctgggc gaagcccgca gtatattgcc cacgtacggt tttttcgcgt
     7681 acgttggagc ggtcgatgcg gcgcagggac ttcagtactt tcactttttc atcgcggatg
     7741 ctgtctgcgc tcaggtcaga tggcggagac atcgcaatca tgcagagaat ttgtagcagg
     7801 tggttctgaa tcatgtcacg catctgaccg gctttatcaa aatagcccca gcgcccttcg
     7861 atccccactt cttctgccac ggtaatctca acatgatcaa tggtgcgatt gtcccagtta
     7921 ttcacaaaca gggagttagc aaaacgcagc gccaacaggt tcagcaccgt ttctttacca
     7981 agatagtggt cgatacggta aacctggcac tcctcgaagt attcgccaac ctgatcattg
     8041 atttcctgcg aggtcgccag cgacgtcccc agcggtttct ccatgactac gcgtgccggt
     8101 ttagcattca gttttgcctc gccaagccct ttgcaaattg cgccaaaagt gctgggcggc
     8161 atggcaaagt agttaatggt gatacgattt ttttgatcca gcatcgcgcc gagacggttg
     8221 aatgcagcag tgtcattgac atcgagatta caaaaatcca gacgtgcact cagggtgtcc
     8281 cataaacctt catcaatggt ttctttcatg aaagtttcga gcgcctcgcg gacaactttg
     8341 gtatatgccg ctttatccca gtcagcacgc cctacgccga taatccgggt gtccgggttg
     8401 agctgaccgg ctttttccag ttgatacagg gaaggcagca atttacgacg cgcaaggtcg
     8461 cctttcgcgc cgaaaatgac caggtcacag gcctgggctg tttgcgttac cgccatgtca
     8521 ttctccttaa gttaactaac ccggtactta agccagggta tacttgtaat tttcttacgg
     8581 tgcactgtac tgcttttacg agcttgcgaa aactgtaaac gcttatccac ccgtgcgatt
     8641 acgggaaaag cgcgcaaagc gcggcaaaac tgataaaaaa tcatcgtttt ccattcggta
     8701 aaaatctgac actgatcatg ttatgaaaaa aaataacaac ttttttatct gcttttgtca
     8761 ttaacacggc acacaggcgt aatatctgac aaaactgcat ttcgatttct ttcagtgcgg
     8821 aaatcgtcat tacccgtgag tctctttaca tcatgaatat gctggaaaaa atccagtctc
     8881 agctggaaca tttgagcaaa tcagagcgca aagttgccga ggtcattctg gcttcgcccg
     8941 ataacgcgat ccattcgagt attgctgcta tggcactgga agccaatgtt agcgaaccga
     9001 cggtgaatcg tttctgtcgc agcatggaca cgcgcggttt tcctgatttt aaacttcatc
     9061 tggcacagag tctggcgaat ggcactccct atgttaatcg caatgtcaat gaagatgaca
     9121 gcgttgaatc atacacaggg aaaatatttg agtccgcaat ggcaacgctt gatcatgtcc
     9181 gtcattcact ggataaatct gccatcaacc gcgccgtcga cttgctcact caggcaaaaa
     9241 aaatcgcctt tttcggatta ggctcttcag ccgccgttgc ccacgatgcg atgaataagt
     9301 tctttcgttt taatgttccg gtggtgtact ccgatgatat cgtgctgcaa cgcatgagtt
     9361 gtatgaattg tagcgacgga gacgtggtgg tgctgatttc tcacactgga agaacaaaaa
     9421 atctggtcga gctggcgcag ctggctcgcg aaaacgacgc aatggtgatt gccctcacct
     9481 ctgcgggtac cccgctcgcc cgggaagcaa cgctggcaat taccctcgac gtaccggaag
     9541 atactgacat ttatatgccc atggtttctc gacttgcaca gctgaccgtg atagatgtgc
     9601 tggcgacagg atttactttg cgacgcggtg caaaattcag agataacttg aagcgggtca
     9661 aggaagcgct gaaggaatcg cgttttgata agcagttact taatttaagt gacgatcgct
     9721 aaaaacgact gtcactgtcc taatcttata cgacatccga atgagattaa tttatcgcca
     9781 tcgcggcgtt atttcattcg gatttcatgt tcaagcaaca cctggttgtt tcagtcaacg
     9841 gagtattaca tgtccagaag gcttcgcaga acaaaaatcg ttaccacgtt aggcccagca
     9901 acagatcgcg ataataatct tgaaaaagtt atcgcggcgg gtgccaacgt tgtacgtatg
     9961 aacttttctc acggctcgcc tgaagatcac aaaatgcgcg cggataaagt tcgtgagatt
    10021 gccgcaaaac tggggcgtca tgtggctatt ctgggtgacc tccaggggcc caaaatccgt
    10081 gtatccacct ttaaagaagg caaagttttc ctcaatattg gggataaatt cctgctcgac
    10141 gccaacctgg gtaaaggtga aggcgacaaa gaaaaagtcg gtatcgacta caaaggcctg
    10201 cctgctgacg tcgtgcctgg tgacatcctg ctgctggacg atggtcgcgt ccagttaaaa
    10261 gtactggaag ttcagggcat gaaagtgttc accgaagtca ccgtcggtgg tcccctctcc
    10321 aacaataaag gtatcaacaa acttggcggc ggtttgtcgg ctgaagcgct gaccgaaaaa
    10381 gacaaagcag acattaagac tgcggcgttg attggcgtag attacctggc tgtctccttc
    10441 ccacgctgtg gcgaagatct gaactatgcc cgtcgcttgg cacgcgatgc aggatgtgat
    10501 gcgaaaattg ttgccaaggt tgaacgtgcg gaagccgttt gcagccagga tgcaatggat
    10561 gacatcatcc tcgcctctga cgtggtaatg gttgcacgtg gcgacctcgg tgtggaaatt
    10621 ggcgacccgg aactggtcgg cattcagaaa gcgttgatcc gtcgtgcgcg tcagctaaac
    10681 cgagcggtaa tcacggcgac ccagatgatg gagtcaatga ttactaaccc gatgccgacg
    10741 cgtgcagaag tcatggacgt agcaaacgcc gttctggatg gtactgacgc tgtgatgctg
    10801 tctgcagaaa ctgccgcagg gcagtatccg tcagaaactg ttgcagccat ggcgcgcgtt
    10861 tgcctgggtg cggaaaaaat cccgagcatc aacgtttcta aacaccgtct ggacgttcag
    10921 ttcgacaatg tggaagaagc tattgccatg tcagcaatgt acgcagctaa ccacctgaaa
    10981 ggcgttacgg cgattattac catgaccgaa tcgggtcgta ccgcgctgat gacctcccgt
    11041 atcagctctg gtctaccaat tttcgccatg tcgcgccatg aacgtacgct gaacctgact
    11101 gctctctatc gtggcgttac gccggtgcac tttgatagcg ctaatgacgg cgtagcagct
    11161 gccagcgaag cggttaatct gctgcgcgat aaaggttact tgatgtctgg tgacctggtg
    11221 attgtcaccc agggcgacgt gatgagtacc gtgggttcta ctaataccac gcgtatttta
    11281 acggtagagt aatctcacct gccccggccg ctgatggccg gggtatttag caacacataa
    11341 attaggacta cttattcaat ccaatcataa tcacagcccc ctgtaaaatg aatacaggga
    11401 tagcctgaat ggagatctaa atagcttgca agacgtcgca tacccgcttc ctctgcaaca
    11461 ctatgtccaa taactgccat cggtaagcca ctatctttgg agtattgtgt ctgcacccat
    11521 gttttaattg aatcgttaat cgtaatataa taatcagcct gatattccat atattgtatt
    11581 ggatcacaaa aacagcctgc cccaatgcca acagttctga ctactctg
//
LOCUS       DAJTSL010000128        11539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTSL010000128 DAJTSL010000000
VERSION     DAJTSL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.128"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            374..1030
                     /gene="pphA"
                     /locus_tag="OO433_003092"
     CDS             374..1030
                     /gene="pphA"
                     /locus_tag="OO433_003092"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCR6051481.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1031..1222)
                     /locus_tag="OO433_003093"
     CDS             complement(1031..1222)
                     /locus_tag="OO433_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6051482.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1327..1563)
                     /locus_tag="OO433_003094"
     CDS             complement(1327..1563)
                     /locus_tag="OO433_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6051483.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1681..3120)
                     /gene="rsmF"
                     /locus_tag="OO433_003095"
     CDS             complement(1681..3120)
                     /gene="rsmF"
                     /locus_tag="OO433_003095"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR6051484.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3200..5833)
                     /gene="yebT"
                     /locus_tag="OO433_003096"
     CDS             complement(3200..5833)
                     /gene="yebT"
                     /locus_tag="OO433_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR6051485.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RSNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGTGKEQDHFVALDTQPKYRLNNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDTGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVSGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISATGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5802..7085)
                     /gene="yebS"
                     /locus_tag="OO433_003097"
     CDS             complement(5802..7085)
                     /gene="yebS"
                     /locus_tag="OO433_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6051486.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7215..7712
                     /gene="msrC"
                     /locus_tag="OO433_003098"
     CDS             7215..7712
                     /gene="msrC"
                     /locus_tag="OO433_003098"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6051487.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7809..8507
                     /gene="proQ"
                     /locus_tag="OO433_003099"
     CDS             7809..8507
                     /gene="proQ"
                     /locus_tag="OO433_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6051488.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8527..10575
                     /gene="prc"
                     /locus_tag="OO433_003100"
     CDS             8527..10575
                     /gene="prc"
                     /locus_tag="OO433_003100"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6051489.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            10767..>11539
                     /gene="htpX"
                     /locus_tag="OO433_003101"
     CDS             10767..>11539
                     /gene="htpX"
                     /locus_tag="OO433_003101"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6051490.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSM"
BASE COUNT         2958 a         2855 c         2977 g         2749 t
ORIGIN      
        1 acataattgc tacggaatta ttcaatacag gaattgcttg cgtctgcatg gattgacctg
       61 aaattttccc gaaaatttct ctaaaaaact cgaaaaaaat ggtaactggt tgaatgtatt
      121 aatatgcaat ggtacgtgtc agggattaaa agatgaacgt aaatttattc aacgcattaa
      181 ttttaaaggg ttttattgtt tgttgacgaa aacaggaatc gtgttcggtc tctttttatc
      241 tgttaaaagc cagaagcatt tccttcgctg actttatagt caaccataac acacacactc
      301 tactgtctga gtccagcgtt ttttaacatt cttgttaaga ttatgtgatc tttagcacgg
      361 gaggaaaata ttgatgaaac agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg
      421 gcgacatatc tggctttctg gcgatataca cggttgtctt gagcagttgc gccgcaaatt
      481 atggcattgt cgttttgatc cgtggcgaga tttacttatc tcagtgggag acgttatcga
      541 tcgtgggccg caaagtttac gttgtctgca gttactggaa caacattggg tttgtgcggt
      601 aagaggcaat catgaacaga tggcgatgga tgcgctggca tcccagcaga tgtctttgtg
      661 gttgatgaat ggcggcgact ggtttattgc gctggcagat aatcaacaga aacaagcgaa
      721 aacggcgctg gaaaaatgtc agcatttgcc ctttattctt gaagtacaca gtcgcaccgg
      781 caagcatgtt attgctcatg cggattatcc agatgatgtt tatgaatggc aaaaggacgt
      841 tgatttgcat caggtcttgt ggagccgctc gcgattaggt gaacgccaaa aagggcaggg
      901 aattacaggt gctgatcatt tctggtttgg tcatacaccg ttgcgacatc gcgtggatat
      961 tggcaacctg cattatattg ataccggtgc tgtctttggg ggcgaactga ctcttgtgca
     1021 attgcaatga ttaaaaatca ccgtactcct gtgcaggtcg ccagaaacca tctataaaat
     1081 cctcaatcgg aaaacaaccg ccctggcgga tccgttgatc gctcatagaa taaagacact
     1141 gctgttccgt gttgtagaca tccacaacaa tatcttcaca accgccatcc aggtagcaaa
     1201 caaaaagtac cagcgcgaac atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag
     1261 gagagatttt acaacggggg aataaccagg acaaataacc cgccatgccg acgggttctt
     1321 tttggatcag gcaagacgca taatccaggc atcagattgt tgatcgtaac gggtacggta
     1381 aaggacagag tgttcgccat ttagcagggc agggatactg gtttcagcat cccaggcatc
     1441 gagttgcata cataaatcgg ggtcagattt acaaggaatg gttaacgttc gatcacccgg
     1501 cgattcaccg tgtaattcgc cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt
     1561 catcttcgtg ctctccattt agctgccggt tgtggtacga ccagtccgat cgcaccattt
     1621 ttcagcctag ttgaggcagc cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg
     1681 tcaggcgtta ccggtaaaaa gtttcccatc gcgcaccagt tcacgcggat agctgttttt
     1741 caatcgcgaa ccaatccgtt tggctaaacc aatcggttga tgctggaaag taaccaacac
     1801 gtcatccgcc actggcgcgg cttgcgggta aacatcgcgc ccgcgatacc actcctccgc
     1861 ttcctgcggt gtcagttcaa aagcgttcac attgtcgggg gtggcaaggg caataactgc
     1921 ttcatgctgc cagcgataac ctttgttgtg cgtttcggca agtttaatcc ccaagcgaga
     1981 aaatcggact ttaccgatca gggcttcaat gcccaccggg aacaaccaca gttctttgtc
     2041 acgctgccag aggcgcaggt tttcatccca gtttaagcca acacctgcag ccgcctggcg
     2101 aatttgtcca gcttcgcgat ctttcaccgg gctgaacgga aaattaccga ctttgtattt
     2161 gggggcgggt aaggcgggaa tcgcctgagt tttacgcaga cgagcaacga agaagccttc
     2221 gcagtcgtaa atttgtggga aaacatgcaa aaagccttct tcggtcagcg ctttgtttgc
     2281 accagggaag agatcgccaa gtggtaaaaa ctctactgcg tcggggtaag tctctttcag
     2341 ccacaggcaa acggcttcgt tttcttcctg gtttaaggta caggtcgagt aaaccagcgt
     2401 accaccagga cgtaatgcat gaaaggcgct gtcgataagc tcccgttgtg tagctgcgat
     2461 ttcctgattg ctttctggtg accagttttt tagcgcatcg ggatctttac gcaccacgcc
     2521 ttcgccggag cagggagcgt ccagcaaaat ggcatcaaac atttctggca ctgccgcacc
     2581 aaacacgcgg ccatcaaaat gtgtgagcgc aacattactg atgccacagc ggctgatatt
     2641 ggcatgtaac acttttaccc gactggcgga aaactcattg gcaaggattg ccccttcgtt
     2701 attcatccgc gcggcaattt gcgtcgtttt tgagcctggc gcagcagcga catccatcac
     2761 ccgctgtggt gcattaccgt cagcaaacaa ggcggcgacg ggcaacattg aactggcttc
     2821 ctgaatataa aacaggccac ttaaatgctc ggcggtacta cccaatggca atgcatcttc
     2881 attgtcgcgt tcaatccaga aaccttcttc acaccacgga attggcgtaa gcgtccagcc
     2941 ataaggagcg gttaattgca ggaaatcagc aacggagatt ttcagcgtat taacgcgaat
     3001 gctgcggcgc aacgggcgct gacaggcggc aagaaaatca tcaaatgaga gcgtcgaagg
     3061 catagcttcg cgcatttgtg tcagaaacgc gtccgggaaa taaacggtgt gttgggccac
     3121 gagcatgtac cactggcaaa aaaacaggcg cgcagtttag cataaacgct ccggcgcagg
     3181 cacgccggag cagtgggcat tatttgggaa gcgcagttcc ccattcacgc cactctttcg
     3241 gttcactttc ctgcaacagg aagtgtttgc cttcctgggc tttcggtgcc agtggcgtac
     3301 ccggaggcgt ggcgaaggcg atgccgccac ggataaactg gttaaaggtg ccggttttca
     3361 ctacgccgcc cgtcagacca aagtccagac tgtaacccga tgccaaccag aagacggaat
     3421 tgttacgcac caggtgttga tagcgtttac tgatgcgcat cgcaatcatc acgcgatctg
     3481 acaatgtccc cagcgtcatt ccggtaaccg tacctacttc cagaccacgg aacagcacag
     3541 gcgtaccaat acctaacgaa ccggcttccg gtgcttcgac aataatgctt aagccatcca
     3601 ggtaacgcga atcagtaata gtagcttctt gcaactcaaa gtcacggcga ggattacccc
     3661 gacctggttc gacattgata tacggctgga ggatagtatc tagatgctca acgcccgtgg
     3721 ctgaaatttg cggtgtgacc accgagaagc gcgtaccgcc gcgggcgaag gtctggacat
     3781 actccggata gagcaccgcc tttgcctgta cttcattgcg cgcggtaatc agatccagcg
     3841 tctggatttg cccgatatca ataccgagat agcgaattgg catcccgacc gccagttttc
     3901 cggcatcgaa agcgtgaagc gtaatctgcc cactaaccgc acgggccgct gtttcggaag
     3961 catacagaat tcgtttgtcg cctttacgct gactggcgct ggcaccgctg aggttatcga
     4021 agctaatggc tccctttaat gctctggaga gcggggatgc ctgtacggtc aggccactac
     4081 cattcagctg aactttcgcc ccgccttctg cccagaacac gctattgctg gtcagaaggt
     4141 tgcgatactc cggcttaata tgcagatcga tatcaaacgc gttagctcgc ggacgcacgg
     4201 taatcacttc accaacttca aatttacggt acagcactac cgatcctgcc tgcacatccg
     4261 gcagcgtctc tgcactcaaa ctcacggttg tggtgggtaa atcgctaagg ctgttctcca
     4321 gcgctttttc cagattggca tacagtggat agctggcttt catctcacct ttatcgcctg
     4381 gcagaatacg tatcccgccg ttaatccatt ctgaggcgct ggcaccaaga aactcaacgc
     4441 catccagccc caccttcacg tcgacacggc tgttgacgac aaatttgcta tcgcctttta
     4501 ccagttcgcg atgctgaggc tcgatggcga cggtaaaggt aacgcctttg ctggtgagtt
     4561 tacgatcaat aacctggcct acctgcacgc cgtgaagaat gagcggctga cccgtatcaa
     4621 taccgtaact ttccggcgcg gtcagggtca gcgtcagaac atcaggttca tgcagcagtg
     4681 ctttttcgcc tggcacaaca acgaactctt tgcgtggctc gccatcgccg ggtaccaact
     4741 cgaaggtttt gccagtcagc agggcgctga gattggcatc gctaagggat aatttcggat
     4801 tacgtaattc gatgcgggta ttttcacgaa gcagggtaac gacgctggga tcaactgtca
     4861 tttccccagt gactttacca ccaggattta aatccagttt agttagctgt ccgacttcca
     4921 gtccctgata cattaatggc gtcgagtcgg cggttaatcc ggccccactc ggcagttcca
     4981 gtttgattat tacgccacgc tggctatggg cgaggtcttc atacagacca aaggtatctt
     5041 ccgcctcggc aggtttcgac tcttctggtg aatcgaaggc aatcgcaccg ttaaccagtg
     5101 ccgccaggct ttccagtttc accttcgcgc cactgatact gacattggca tcaacgccgg
     5161 aaacgttcca gaaacggcta ccttttttca ccagatcggt aaaacgccgc tcgatcagga
     5221 catcaatcac cacgccttgt ttgttgggat tgatggcata gtcgtagact tttcccaccg
     5281 ggatcttgcg gaaatagacc aatgaaccgc tgttcagcga accgagatcg ggggcttgca
     5341 ggtggatcat cagatcgcca ttgttcagcc gatatttcgg ttgggtatcg agtgcgacaa
     5401 agtgatcctg ctctttacct gtacccggca tcatgccgat atagttccca ccgacgaggg
     5461 cgtccagccc ggagacacct gccaacgatg cttttggcgt caccagccag aattgagtct
     5521 cttcgcgcag cgcatctttc atatcggact tgatgctgac cttgacttca atcttacgaa
     5581 gatcgtcgct gaggctgata tcctgcactg ttccgacttc aacgccctga taacgaacag
     5641 gcgtacggcc cgggacaata ccatccgccg acataaagtc gatggtgacg gtattacttc
     5701 ggtcctgata actgtcccaa atcagccaac cggcaatcat tagcgcgatg aaaggcagca
     5761 gccagaaagg tgagatacgg cgtttatttt taatctgcgc ttcagtcgtc gaagcgggcg
     5821 tttcctgact catgtgcatc ccaaagtaag cggctgtcca gccattccac agcaagaata
     5881 gtcaaaatta cggctgcgcc aaaataaaac gcagccggtc ccatagtaaa agcgaggatc
     5941 tgatcgcgat taatcagcga catggttaaa gatatgacaa acaggtcgag catcgaccag
     6001 cgtccaatcc aggtcaccat ccgcagtaac agaatgcgtg tgcgtaaacc ttgctggcat
     6061 ttaaaatgaa tgctcaacag taaagtgaac atgacgatca ctttagtaaa cggtaccaga
     6121 atactggcga taaacacgat tccggcgacc gcaatgttgc tacttgccag cgacataatc
     6181 ccggaaagaa tcgtatcttc ctgccggcca ccattcagat aaatgataga aataggcaat
     6241 aggttggctg gtaacaacaa aacgattgac gctaacagcg ccgcccagca tttttgcaaa
     6301 ctatgacgac gacgcaggcg tagcgggata tggcaacgcg ggcagcgacc acgctgatca
     6361 ggatagccgg taaaatggca cccaagacag acacgaagtt tctcgtccct acgcgtagcg
     6421 gggcgctgcg gataatatcg ctcccacagc tcctcaacat taagatgtga caacgtcacc
     6481 gtcgttaaaa tcaccaacgc cacaaaagag aacaagccga cacccgcctg gatatgggca
     6541 taatcctgta cctttataga agcaacgcca atgccgacca ggtagatatc cagcattacc
     6601 cactctttaa gacgctcaag catcagcagt accggacgta aattcattcc cagtcggtta
     6661 ccaaaccata aatatgctat ggaggacacc agaatgaggg gggcaccaat aacgcagaaa
     6721 aagaccatcg cccccgttat cgtatcgccc tgtttggtca tttgccagat gccttgcatg
     6781 acgtttgcgt cgatacgaat acctaacagc cagatatgca acagcggttc gccccaggca
     6841 aacggcatca gcaacagcat ggtgaaagcc attgccgcca ggcgcgttag cgaccagtcg
     6901 cgcccgtcgc gaatttttgc ctgacagcgc ggacaatagg cactctgatg agaatttatc
     6961 tcgggcaggc taaacagcat gtcacattgc gggcaacgtt ggtaatcacc acgcggcagt
     7021 tcctcgccga ttgacctcac tgttatcttt tttgtcggcg taatttgtgg tgtgttaaga
     7081 gccatgacgt gccaaatgta ataagaatta acattaatct taacccatga tgggaatgat
     7141 cttgagcatt aacgcattta atgcttattt taataggctg gcagttccag gtaagacaaa
     7201 caagtgatta tataatgaac aaaacagaat tttacgcgga tttaaatcgc gactttaacg
     7261 cgctgatggc gggagaaacc agttttctgg caacgcttgc gaacaccagt gcgttgttat
     7321 acgagcgtct cactgacgta aactgggcag gtttttattt gcttgaggac gatacactgg
     7381 tactcggacc atttcagggc aaaattgcct gtgtccggat acccgtcggg cgcggggtgt
     7441 gcggcactgc ggttgcccgc aatcaagtgc agcgtatcga ggatgttcac gcgtttgacg
     7501 ggcatattgc ctgtgatgcg gcgagtaatt ctgaaattgt tctgccgctg gtggtgaaaa
     7561 atcagattat tggtgttctt gacatcgata gtaccgtctt cggtcgcttt acagacgagg
     7621 acgagcaagg cttacgtcag cttgtggcac agcttgaaaa agtgcttgca acgacggatt
     7681 acaaaaaatt ctttgcgagc gtcgcaggat aatcaacgga taacgtagca tttactggta
     7741 gcgtcattat aatgacgcct gttcatgcct gcgcttgttg gctacgtccg ttgtaatcag
     7801 gaaatttcat ggaaaatcaa cctaagttga atagcagtaa agaagtaatc gcgtttctgg
     7861 ccgaacgttt tccccactgt ttcagtgcgg aaggtgaagc gcgtccgctg aaaatcggta
     7921 tttttcagga tttggtcgat cgtgttgccg gggaaatgaa cctgagcaaa acgcaattac
     7981 gatccgcttt acgtctctac acttcgagct ggcgttatct ttacggtgtt aaacccggcg
     8041 caacgcgtgt cgatcttgac ggcaacccat gcggtgagct ggacgagcaa catgtagagc
     8101 atgctcgcaa gcagcttgaa gaagcgaaag cgcgtgttca ggcacagcgt gctgaacagc
     8161 aagcgaaaaa acgcgaagct gccgcagctg ctggtgagaa agaagacgca ccgcgccgcg
     8221 aacgcaagcc acgtccgacc acgccacgcc gcaaagaagg cgctgaacgt aaacctcgtg
     8281 cgcaaaagcc ggtagagaaa gcgccaaaaa cagtaaaagc acctcgcgaa gaacagcaca
     8341 ccccggtttc tgacatttca gctctgactg tcggacaagc tctgaaggtg aaagcgggtc
     8401 aaaacgcgat ggatgccacc gtattagaaa tcaccaaaga cggcgtccgc gtccagctga
     8461 attcgggtat gtctttgatt gtgcgcgcag aacacctggt gttctgaaac ggaggccggg
     8521 ccaggcatga acatgttttt taggcttacc gcgttagctg gcctgcttgc aatagcaggc
     8581 cagaccttcg ctgtagaaga tatcacgcgt gctgatcaaa ttccggtatt aaaggaagag
     8641 acgcagcatg cgacggtaag tgagcgcgta acgtcgcgct tcacccgttc tcattatcgc
     8701 cagttcgacc tcgatcaggc attttcggcc aaaatctttg accgctacct gaatctgctc
     8761 gattacagcc acaacgtgct gctggcaagc gatgttgaac agttcgcgaa aaagaaaacc
     8821 gagttaggcg atgaactgcg ttcaggcaaa ctcgacgttt tctacgatct ctacaatctg
     8881 gcgcaaaagc gccgttttga gcgttaccag tacgctttgt cggtactgga aaagccgatg
     8941 gatttcaccg gcaacgacac ttataacctt gaccgcagca aagcgccctg gccgaaaaac
     9001 gaggctgagt tgaacgcgct gtgggacagt aaagtcaaat tcgacgagtt aagcctgaag
     9061 ctggcaggaa aaacggataa agaaattcgt gaaaccctga cacgccgcta caaatttgcc
     9121 attcgtcgtc tggcgcaaac caacagcgaa gatgttttct cgctggcaat gacggcgttt
     9181 gcgcgtgaaa tcgacccgca taccaactat ctttccccgc gtaataccga acagttcaac
     9241 actgaaatga gtttgtcgct ggaaggtatt ggcgcagtgc tgcaaatgga tgatgactac
     9301 accgttatca attcgatggt ggcaggtggt ccggcagcga agagtaaagc tatcagcgtt
     9361 ggtgacaaaa ttgtcggtgt tggtcaaaca ggcaagccga tggttgacgt gattggctgg
     9421 cgtcttgatg atgtggttgc cttaattaaa gggccgaagg gcagtaaagt tcgtctggaa
     9481 attttacctg ctggtaaagg gaccaagacc cgcactgtaa cattgacccg tgaacgtatt
     9541 cgtctcgaag accgcgcggt taaaatgtcg gtgaagaccg tcggtaaaga gaaagtcggc
     9601 gtgctggata ttccgggctt ctatgtgggt ttgacagacg atgtcaaagt tcaactgcag
     9661 aaactggaaa aacagaatgt cagcagtgtg atcatcgacc tgcgtagcaa tggcggtggg
     9721 gcgttgactg aagcggtatc gctctcaggt ctgtttattc ctgcgggtcc cattgttcag
     9781 gtccgcgata acaacggcaa ggttcgtgaa gacagcgata ccgacgggca ggtgttctat
     9841 aaaggcccgc tggtggtact ggttgaccgt ttcagtgctt cggcttcaga aatctttgcc
     9901 gcggcaatgc aggattacgg tcgtgcgctg gttgtgggtg aaccgacgtt tggtaaaggc
     9961 accgttcagc aataccgttc attgaaccgt atttacgatc agatgttacg tcctgaatgg
    10021 ccagcgctgg gttctgtgca gtacacgatc cagaaattct atcgcgttaa cggcggcagt
    10081 acgcaacgta aaggcgtaac gccagacatc atcatgccga cgggtaatga agaaacggaa
    10141 acgggtgaga aattcgaaga taacgcgctg ccgtgggata gcattgatgc cgcgacttat
    10201 gtgaaatcag gcgatttaac ggcctttggg ccggagctgc tgaaggaaca taatgcgcgt
    10261 atcgcgaaag atcctgagtt ccagaacatc atgaaggata tcgcgcgctt caacgctatg
    10321 aaggacaagc gcaatatcgt ttctctgaac tacgctgtgc gtgagaaaga gaataatgaa
    10381 gatgatgcga cgcgtctggc gcgtttgaac gaacgcttta aacgcgaagg taaaccggag
    10441 ttgaagaagc tggatgatct accgaaagat taccaagagc cggatcctta tctggatgag
    10501 acggtgaata tcgcactcga tctggcgaag cttgaaaaag ccagacccgc ggaacaaccc
    10561 gctcccgtca agtaatatca atcaggcaca agaaattgtg cctgattttt taacagcggc
    10621 aagatgccgt aaatcagatg ctacaaaatg taaagttgtg tctttctggt gacttacgca
    10681 ctatccagac ttgaaaatag tcgcgtaacc catacgatgt gggtatcgca tattgcgttt
    10741 tgttaaactg aggtaaaaag aaaattatga tgcgaatcgc gctcttcctg ctaacgaacc
    10801 tggccgtaat ggtcgttttc gggctggtac tgagcctgac agggatacag tcgagcagcg
    10861 ttcaggggct gatgatcatg gccttgctgt tcggttttgg tggttccttc gtttcgcttc
    10921 tgatgtccaa atggatggca ttacgatctg ttggcgggga agtgatcgag caaccgcgta
    10981 acgaaaggga acgttggctg gtcaatactg tagcaaccca ggctcgtcag gcagggatcg
    11041 ctatgccgca agtggctatc taccatgcgc cggacatcaa cgcttttgca accggtgcgc
    11101 gccgtgatgc ctctctggtt gctgtcagca ccggtttgct gcagaacatg agcccggatg
    11161 aagccgaggc ggtaattgct cacgaaatca gccacatcgc caatggtgat atggtcacca
    11221 tgacgctgat tcagggcgtg gtgaacacct tcgttatctt tatttcccgt attctggcgc
    11281 agcttgccgc gggttttatg ggcggaaatc gtgatgaagg tgaagagagc aacggcaacc
    11341 cgctgatcta ctttgcggtt gcaacggttc tggaactggt gtttggtatt ctggcgagca
    11401 ttatcaccat gtggttctcg cgtcatcgtg aattccatgc tgatgccggt tcggcaaaac
    11461 tggttggtcg cgagaaaatg attgccgcgc tgcagcgcct gaaaaccagc tatgaaccgc
    11521 aagaagcaac cagcatgat
//
LOCUS       DAJTSL010000129        11400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTSL010000129 DAJTSL010000000
VERSION     DAJTSL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.129"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..742)
                     /gene="mak"
                     /locus_tag="OO433_003102"
     CDS             complement(<1..742)
                     /gene="mak"
                     /locus_tag="OO433_003102"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6051491.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDV"
     gene            867..1778
                     /gene="rdgC"
                     /locus_tag="OO433_003103"
     CDS             867..1778
                     /gene="rdgC"
                     /locus_tag="OO433_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6051492.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(1856..1939)
                     /locus_tag="OO433_003104"
     CDS             complement(1856..1939)
                     /locus_tag="OO433_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051493.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIVRRSE"
     gene            complement(1936..2277)
                     /locus_tag="OO433_003105"
                     /pseudo
     CDS             complement(1936..2277)
                     /locus_tag="OO433_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(2425..2709)
                     /gene="ppnP"
                     /locus_tag="OO433_003106"
     CDS             complement(2425..2709)
                     /gene="ppnP"
                     /locus_tag="OO433_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6051494.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(2781..3458)
                     /locus_tag="OO433_003107"
     CDS             complement(2781..3458)
                     /locus_tag="OO433_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6051495.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(3716..3907)
                     /gene="yaiA"
                     /locus_tag="OO433_003108"
     CDS             complement(3716..3907)
                     /gene="yaiA"
                     /locus_tag="OO433_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6051496.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(3957..4481)
                     /gene="aroL"
                     /locus_tag="OO433_003109"
     CDS             complement(3957..4481)
                     /gene="aroL"
                     /locus_tag="OO433_003109"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6051497.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(4664..5122)
                     /locus_tag="OO433_003110"
     CDS             complement(4664..5122)
                     /locus_tag="OO433_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6051498.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5242..6051
                     /gene="proC"
                     /locus_tag="OO433_003111"
     CDS             5242..6051
                     /gene="proC"
                     /locus_tag="OO433_003111"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6051499.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPNKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(6068..7183)
                     /gene="adrA"
                     /locus_tag="OO433_003112"
                     /gene_synonym="dgcC"
     CDS             complement(6068..7183)
                     /gene="adrA"
                     /locus_tag="OO433_003112"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6051500.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(7285..7605)
                     /gene="psiF"
                     /locus_tag="OO433_003113"
     CDS             complement(7285..7605)
                     /gene="psiF"
                     /locus_tag="OO433_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6051501.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(7724..9139)
                     /gene="phoA"
                     /locus_tag="OO433_003114"
     CDS             complement(7724..9139)
                     /gene="phoA"
                     /locus_tag="OO433_003114"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6051502.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(9240..9500)
                     /gene="iraP"
                     /locus_tag="OO433_003115"
     CDS             complement(9240..9500)
                     /gene="iraP"
                     /locus_tag="OO433_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6051503.1"
                     /translation="MKNLIAELLLKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIEQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            complement(9727..9884)
                     /locus_tag="OO433_003116"
                     /pseudo
     CDS             complement(9727..9884)
                     /locus_tag="OO433_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9962..11056
                     /gene="ddlA"
                     /locus_tag="OO433_003117"
     CDS             9962..11056
                     /gene="ddlA"
                     /locus_tag="OO433_003117"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6051504.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPSKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLRLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(11080..11292)
                     /locus_tag="OO433_003118"
     CDS             complement(11080..11292)
                     /locus_tag="OO433_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6051505.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGAVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
BASE COUNT         2841 a         2954 c         2723 g         2882 t
ORIGIN      
        1 tcacatccgg atcgagaata ttcacgacat gtgccagcga ttttgccagc cgcagctcgt
       61 agcgacgcaa tgccagttcc gctaccggat cgctttcttc aaccaggcgg ataatttcac
      121 tgcctttcag cgcatgtccg ctcaaacgac gataatccat cgcgaatccc gtgcccgaaa
      181 taaaggtttc aatacaacct tgtttaccgc aataacaagg gacttcctcg cgataacgca
      241 gttcgtcttc gtccatccac ggtagcggat tgtgtcccca ctcacctgcc gtgccattgc
      301 cgctgatatg cgcccgccca ttgaatgcca cgcccgcgcc gcatcccgtg ccgataatca
      361 cggcaaatac cgtctgcgct cccgctgccg cgccatctac tgcttctgaa accgccagac
      421 agttagcgtc atttgccagc cgcacttccc gctgcaacct cgcgcttaag tctttatcga
      481 atggctgacc gttgagccag gttgaattgg cattcttcac cacaccggtg taaggcgaaa
      541 ttgagccagg aatgcccata cctaccgttc cgcgctgccc cgtcgcctgc tccgccatat
      601 caaccaacgt ggcgatcgtt tcaatagtct gccggtaatc atcacgcggc gtgggcagac
      661 gatggcggta caactgctcc cctgcatcgc ccagtgcaat cacttcagtt ttggtgccgc
      721 ctaaatcgat acctatacgc acggtactct ccttattttt ttcaatatca atagcgtaga
      781 gacggacaac cggattggca atgcaaggcc gccgacaatt cgttatcatg cccgctaaat
      841 ttaacaacaa ggccgtggaa attatcatgc tgtggttcaa aaatttaatg gtttaccgtc
      901 ttagccgcga gatttcgctg cgtgcagaag agatggaaaa acagctagcc tcgatggcat
      961 ttaccccatg cggcagccag gacatggcga agatgggctg ggttcctccg atgggatcgc
     1021 acagcgatgc gttaacgcac gttgccaatg gtcaaattgt tatctgcgcg cgcaaagaag
     1081 aaaaaatcct cccgtctccg gtgattaagc aggcgctgga agcgaaaatc gccaaactgg
     1141 aagcggaaca ggcgcgtaag ctgaagaaaa ccgaaaaaga ttcgctgaaa gacgaagtgc
     1201 tgcactctct gttgccgcgt gctttcagcc gttttagcca gacaatgatg tggatcgaca
     1261 cggttaacgg tttgattatg gtggactgcg ccagtgccaa aaaagcggaa gatacgctgg
     1321 cactgctgcg taaaagcctg gggtcgttac ctgtggtacc attgagcatg gaaaacccga
     1381 ttgaactgac gctgaccgaa tgggttcgct ccggtagcgc ggcacagggc ttccagctgc
     1441 ttgatgaagc cgagctgaaa tcgttgctgg aagatggcgg cgtgatccgc gcgaagaaac
     1501 aagatctgac cagcgaagag atcaccaatc acatcgaagc cggaaaagtg gtgactaaac
     1561 tggcgctcga ctggcagcag cgcattcagt ttgtgatgtg cgacgatggt tcgctcaagc
     1621 gtctgaagtt ttgcgacgag ctgcgcgatc aaaacgaaga tatcgaccgt gaagatttcg
     1681 cccagcgttt tgatgccgat ttcatcctga tgactggtga actggcagcg ttaattcaaa
     1741 acctgattga aggattaggt ggcgaagcac aacgttaatt gctgattttc ctttaatgcc
     1801 ggatgcgacg cctgccgcgt cttatccggc gtacgaagcc acaccaggga tataattatt
     1861 cgctacggcg gacaataatt tttagcgcag caatattatg cgttttatgc tgtaacttgc
     1921 tccatggacg ttgtgtcatt gtttttcctc aagccagtga cgcgcttcac tgcgaatttt
     1981 ccgcattgcc ccggttttca ctaaatcccg taacagcgaa agatcgggtt gttcgacaaa
     2041 taagagtaat gatggatcct cttttaacca cgtcgttttc acatcggcag cgagctgtgg
     2101 attattcata gctagcgacc gatgccgaga ctcggtcaat gtcgttaaca acgacgccgg
     2161 ggtatgtgcg ttgcgtaatg ccgtttggcg caaatgatca ttgcgacttt gcagcaatgc
     2221 acgccaccat tcatcatctg atttatcttt tatcgtagcc agttccgcaa caatatctgg
     2281 agatcttcac tgatatttat atcaaccttg atgaattatg aatatatcgg aaattgaaaa
     2341 aatcgcagca aaggaaatat tattatgggt taaagagagg atatttcaga aaaatgccct
     2401 cccgttcaag gggaaggcga ggaattacag atagcggcac agataagagg tgggttcggc
     2461 aacttgcaga tgaaactcac tgtgaccggg aacattaaac accgaaccgg cttcatacac
     2521 ctgccagtcg gtcgcatcag gcagtaacac attcagcgcg ccactgatta ccgtcatctc
     2581 ttccggctca gcggtgctga aggtgtattc gccttcaacc ataacaccca cgctggcgcg
     2641 accagtgctg ctgctggaaa agccgattga tttcactttg ccggaaaagt actcattact
     2701 ttgaagcata aactggccct gtctggtagt tgaacatcta cttcactata ggggccagag
     2761 gcgctggctg tcacgcaaaa ttacaccagt aattccgcag ccagccgtgc aatcaatacg
     2821 ttagacagca agacaggaac atcgagctgt ttttgcagta aatcgcgatg acgttggtga
     2881 aatcctaaac aatccagcat gatgacatca gccccttttg ccagtaattc tttccctgca
     2941 tcaatgattt tttgttctga atcatgaatg gggttaccca atgaaaatac cggcgatttc
     3001 tgcaaaattt gccatttttg cgcctgaacg ggcagcatct cctcaaccgg aacgataacc
     3061 cccacctgat gatcttcaac aatagaggaa accagtggag gcaatattcg cgacggctca
     3121 agaaagatcg tattacgcgc agtcatacta ctaatgtttg ctgtactcat taataaaatg
     3181 acgtcataac cctgattatc gagcacttca accacacctt gcaggtctcg ctccacttta
     3241 cgacgcgaaa catgggccag ctggttgtca tttaataatg tgagaatggt gtcttcgcct
     3301 gcttctggcg cgtactccgc catcacttct tcacgactta acttccccag caggctatga
     3361 tgggaaatat tatcttcgtc aatgtattcc gtcaggagcg gcaaaacctc ctgcataggt
     3421 acaatgccga tggtcaggat cgccaacgac gcactcattg ttcatccacc ttacttttct
     3481 ttcgtcgtta attaccgggc aagtgtgaag caccactgct gacacttact tgtgctacaa
     3541 atgacaaaaa gcgtagcagc agacgtgcac ggcataatgt aaagattcca aatgattcca
     3601 gtaatggatt gttattgttt aatattctaa ttattagaaa aacatgaatt atgaaaaatg
     3661 tgacgcagat cccttgtagt ggcactcaga atcccttccg agcagtctga tgcggtcact
     3721 ctttgtcagg gtcctcatag cgttttttcg catccatcgc ttcctgagcg gtcggatgtt
     3781 cactgatcaa cgagtctggt ttaggatgat cggctctgag ttggtaccag gttaccgtct
     3841 gtcctggctt tcctttttca atcgtcacta tatatgcttc acgaggataa ggcggtttcg
     3901 ttggcatcgt tcttccttat ttcacggaat gaacgttaag tataggcgct ctaaaatcaa
     3961 caattgatcg tctgtgccag ggcgctacga atttcagaaa tcacctggct gggttcgttt
     4021 gttgcgtcga tgataatatg cgcaacttcg cgatatagcg catcgcgttc ttccagcact
     4081 tcctgaactt cttcgctcag cggttttccc gttaaggttg gccgtaaatc ttcttccggt
     4141 gcagcttgca gtcggttaac caggactgat actggcgcac acaaataaac cacgatcccg
     4201 ttattttgca tgaagtgacg attaaattcc gtcagaataa tgccgccgcc tgtagcgata
     4261 acggtggatg gcgcagttac cgcttccagc gccgccgttt ctctggcgcg aaatcccgcc
     4321 cactcttccc tttcgacgat ctccgcgacc gtcatattga gctgtgattg caaccactga
     4381 tcggtatcga caaaccgacg gttaagcgaa tcggcaaggg ccattccgac cgttgtttta
     4441 ccacagcccc gaggcccgat cagaaaaaga ggttgtgtca tcgtgggttt tccccaatag
     4501 gtcgcaatgc ggcgaaagcc ggtgtcatga gaatagcgat cattccatca aactagtaca
     4561 atttcgattg taaagaaaaa attccactta aagtgaaaat ctcaatacat cccttactat
     4621 accaataaat attcaagaat gaagtgtaaa taataaatta catttagcca cgactacgtt
     4681 gcacttccag ccaccacttc tccagctccg cggcaaaggc ctggcggtca cgttgtgaaa
     4741 ggctatctgg tccgccggtc tggatcccac tggcacgtaa ggtatccata aaatcgcgca
     4801 tcgtcaggcg ctcacgaatg gtcgctggcg tgtaacgttc gccgcgcgga ttaagcgccg
     4861 ctgcgccttt ctcgatggct tcagcagcca aaggtatatc tgcggtgatc accaaatcgc
     4921 ccgcttcaca ctgccggaca atttcgttat cggcaacgtc gaaacctgcc gcgacgcgca
     4981 gcgtacgaat aaatcgcgat ggcggcacgc gtaaactctg gtttgctacc agtaccagcg
     5041 gcatctgcat acgttccgcc gcgcgataca aaatctcttt aattacattg ggacacgcgt
     5101 cagcatccac ccaaattgtc ataaagtcat cctttgttgg gtaatcctct attgtgtcgc
     5161 gcttttgcct tccggcatag ttctgtttat gcttctgcca gcgattatca aaacaatgaa
     5221 tttcacggca ggagtgaggc aatggaaaag aaaatcggtt ttattggctg cggcaatatg
     5281 ggaaaagcca ttctcggcgg tctgattgcc agcggtcagg tgcttccagg gcaaatctgg
     5341 gtatacaccc cctccccgaa taaagtcgcc gccctgcatg accagttcgg catcaacgcc
     5401 gcagaatcgg cgcaagaagt ggcgcaaatc gccgacatca tttttgctgc cgttaaacct
     5461 ggcatcatga ttaaagtgct tagcgaaatc acctccagcc tgaataaaga ctctctggtc
     5521 gtttctattg ctgcaggtgt cacgctcgac cagcttgccc gcgcgctggg ccatgaccgg
     5581 aaaattatcc gcgccatgcc gaacactccc gcgctggtta atgccgggat gacctccgta
     5641 acgccaaacg cgctggtaac cccagaagat accgctgatg tgctgaatat tttccgctgc
     5701 tttggcgaag cggaagtaat tgctgagccg atgatccacc cggtagtcgg tgtgagcggt
     5761 tcttcgccag cctacgtatt tatgtttatc gaagcgatgg ccgacgccgc cgtgctgggc
     5821 gggatgccac gcgcccaggc gtataaattt gccgctcagg cggtaatggg ttccgcaaaa
     5881 atggtgctgg aaacgggaga acatccgggg gcactgaaag atatggtctg ctcaccggga
     5941 ggcaccacca ttgaagcggt acgcgtactg gaagagaaag gcttccgtgc cgcagtgatc
     6001 gaagcgatga cgaagtgtat ggaaaaatca gaaaaactca gcaaatcctg atgactttcg
     6061 cctgacgtca ggccgccact tcggtgcggt tacgtccggc tttctttgct ttgtaaagcg
     6121 ccaaatctgc cgatttcaac cactcacgat agtgactcat ttgtgggttc agcggcgcaa
     6181 cccccacact aatccgtaaa gttacctgtg gcgtattcgg caagcgtaac gtgtttagcc
     6241 cttcacgcac ccgtaacatg gcggtaatgg cgctctcagc tggcgtaccg gacatgatta
     6301 ctgcaaactc atcgccgcca aaccgaccaa tcacatcgct accgcgcagg gtaatttgta
     6361 actgtcgggt aagcgccaca atcgcttcat cgcccacatc atgaccccag gtatcgttga
     6421 tgctcttgaa atggtcgata tcgataatca gtaacgttgc atcgcgatta tgccgccgac
     6481 agttatcaaa ttcattgcgt aacatagttt cccaatgacg tcggttatac acgcccgtca
     6541 tgccgtcgcg ggtactcatg acctgcaacc tgcgtttatg ttccgccagt ttggttgccg
     6601 tctggtagct gacccagcca aacagcagag gataaacgac aataatggga agggagagcc
     6661 accattccag cggcgcacta ttgaacgaca cggtaatgcc cgtaagctcg agggtgacaa
     6721 ggcaggaaac caccatcaac accagacccg cgacaaacag acgggggccg cctgccccca
     6781 tcaaattcag acacataatc atcaacatag cggtggaagg cagcacgttt acgcccatta
     6841 cgcctaccca cattcccgct aatactgcat cggtttttaa gttgtaaatt tcccggctaa
     6901 gcggatcgac ggccctgctc gctatctgcc aggctaaatg cggccagacg aacgcccagc
     6961 cgaccaacac tagccaccac cagcccggcg gcgggtgcga aaccagcgtt gaagcaatcg
     7021 gtaagaacat gccagccagg ccaaccgcac ggggtagtct gacgcgacgg gcgaagcgca
     7081 gcccggaccg ctgatgttcg ttttgaggag ttaaaggagg ttcctcccca tgcgccgccg
     7141 cttttttgaa aaagttttca tcattcatta tttttgggaa cattcagaaa caattttccc
     7201 aaattataga gacggggcta aaggcagaag tatgaaattt cgggtgagcc actttcgtag
     7261 ctcacccgtt agcactatca ggtatcaggc ggctttcttg aggcaggcac tcataaactt
     7321 attacgatca tcacctttca gagattgttg cgttgcttga ttattacatt cgcgcatctt
     7381 ttgctgctgt ggcgtcaaac ttttttcacc aggcgcagac ttgctgttct tcaggcaatc
     7441 actcatgtag gtcttacgag catccccttt caacgcctgc gccgtcgcct gctgattaca
     7501 ggaggtcata cgctgttgtt gtggggttaa cgttctctcg gcagcgccga ctgtggttaa
     7561 aaaaaccaga ccgaaaagca aggtaaccag taatgtaatt ttcatagcac catccctctt
     7621 catgttttaa ccatgagcgt atgcgcccga gatttgccat taagtctggt tgctaacagc
     7681 aaaaaaacca cccggcagcg aaaattcact gccgggcgcg gttttatttc agccccaggg
     7741 cggctttcat ggtgtagaag agatcggtct ggtcggtcag tccaacgaca ttggcggcat
     7801 gcgggccata cgccgcaata cgcaactgac tgccggtatg ttcttgtgaa tcctcttcgg
     7861 agttcccgta gctcatcacc atcactgcgc catctttggt atttagcgcc tgggtgaggc
     7921 ccggagcttt ggtatccggc gcgacaatct ggctggcgtg ggcgtgatca gcggtgacta
     7981 tgaccagcgt gttgccatcc tttttagcga attccagcgc ccgttgtacg gcttcatcga
     8041 gatcgaccgt ctcgccaatt tgcccacaag gattcgcagc gtgatcctgt ttatcgattg
     8101 acgcaccttc aacttgcagg aaaaatcctt tctcattttt actcaacaat tcaatggctt
     8161 tgtcggtcat ctgcgccagg gttggtacac tgtcattacg ttgcggatta ggcgtacagg
     8221 tgactgcggg cttatcgata ttgccgtggt acgttgcttt cggtcctagc cagcgcactg
     8281 gcatattgcc gtcagcaaac agtccaagca gaggtttttg ctgattcgct tccgtcaccg
     8341 catttagcga ggcggcatcg ctgaccaact gataaccacg cgcctgtgcc tgttcacgca
     8401 gcgtttttcc ctgccattca ccggcggttg ccgtttcagc aaaggttttt gcgccaccgc
     8461 caagcgtaac gtcggcacga gcattaagca gctgttcggt aatcgatcct tttccgcctt
     8521 tttccagagc gttacccgga catttttcac tggtcgcgct cggaccgtag catttgcgcg
     8581 aagtcacatg tgccaccagc gcagcgggcg tggcatcctg caactctgcg gtagaaacgt
     8641 taccggtcgc cagacctgcg gcttttgcca tttccagaat cgttgggtga tctttttcgt
     8701 gaatatcgac gcccagcgcg ccgttatagg ttttgacacc ggttgaccag gcggttgctg
     8761 atgcagccga atcggtgacg tagtccggtt tgccggtttt tttattcagc gcatagtgag
     8821 tgtattgccc ggtaagcggt aaggcatcga tacctttaaa aaagccgccc gcaccttcgg
     8881 cataattacg tgcggctgta atttccgaat cccccatccc atcgccaatc agcaaaataa
     8941 tattttttgc aggtttatcg ctaagagaat cacgcagagc ggcagtctga tcacccgtta
     9001 aacggcgagc accgccgggt gcagtaatat cgccctgagc agcccggttt tccagaacag
     9061 gcatttctgg tgtccgggct tttgtcacag gggtaaacag taacggtaag agtgccagtg
     9121 caatagtgct ttgtttcact ttattttctc catgtacaaa tacattaaaa aataaaaaca
     9181 aagcgactat aagtctcggc cgtgacaact ttatgacagc tgttgaaaag attaactttt
     9241 tactgacgag gatgcttcaa taacttcttt acataattgc gcagcagctc cgtatcgtcg
     9301 tcaggaatgc tggcatcagg ctttacctcg taaagagccc cctccacctg ttcaatcaag
     9361 cgctgttggt cattttgtgc catattgcga agcattgcag tgacgataat ctccaaagct
     9421 tctacctgcg cgcacagttc tttcgactct tcttcttttt gggcaagctt taataacaac
     9481 tcagcaatga gatttttcat gtctgtattt ccttatccaa cgtatggaga agttaacatt
     9541 atgtttcaca acttcatagt gtctggaagt attattttta tgacaaagga aatactttgc
     9601 gcaaatgttc tttgtttgat taccagcgaa acgtttctca tatttaaata attaaccaca
     9661 caaagaacag gcctttaatt atcaaccttt attataaaca aaagttatga aatatattct
     9721 ctgcgtttac ttacaactaa gagaaaaaac tcaccgcatg gccatttaag ccaggaataa
     9781 attagcttcc cgttcatcat ttttgtgatc tcgcgcacat ctttgtaata acgcactttc
     9841 ttcaccatta cgttgcacgc tttgtcagca gaagattgca acattctgtc agaaggccgc
     9901 gaatcccccg ttttgtcgtg gtaatgtatg cactttgtgt tagacgggat aggtttttaa
     9961 gatggaaaaa ctgcgggtag gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc
    10021 tctgcaatcg gcaaaaaaca ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct
    10081 gctgggcatt gataaacaag ggcaatggca cgtcagcgat gccagcaatt atctgctaaa
    10141 tgcagacgat cctgcccata ttgcgttgcg cccttcggcg actagccttg cgcaggtgcc
    10201 aagtaaacat gagcatcagc ttatcgacgc gcaaaacggt cagccgttac cgacggtgga
    10261 tgtcattttc ccgattgtcc acggtacgct gggcgaagat ggttccttgc agggaatgct
    10321 gcgggtcgcc aatttaccgt ttgtaggttc tgatgttctg gcttcagcag cctgtatgga
    10381 taaagatgtt accaaacgtc tgctgcgcga tgccgggctg aacattgcgc catttattac
    10441 cctgacgcgc gccaatcgtc acaacattag ttttgccgaa gtggagtcta aactgaggtt
    10501 accactgttt gtaaaaccgg ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag
    10561 tgaagaacag tacgcaattg ccgtcgatct ggcgttcgaa ttcgatcata aagtgatcgt
    10621 tgagcaaggg atcaaaggtc gtgagatcga atgcgcagtt ctgggcaacg acaacccgca
    10681 agccagcacc tgtggcgaga tcgtactcac cagcgacttc tatgcctacg acaccaagta
    10741 cattgacgaa gatggcgcga aagtggtagt tccggcagcc attgcgccag aaatcaacga
    10801 taagatccgg gcgattgccg ttcaggctta tcaaacgttg ggatgcgcag gcatggcgcg
    10861 tgtagacgtg tttttaaccc cagagaacga agtggtgatc aacgagatca acacactgcc
    10921 tggcttcacc aacatcagta tgtatccgaa gctgtggcaa gccagcggtc tgggttacac
    10981 cgatctgatc acacgtttga ttgagctggc gctggagcgt cacgctgcgg ataacgcatt
    11041 gaaaaccaca atgtaatatt ttaacgcccg ataatattct tattgggcgt tttcagtttc
    11101 ttcatcgcga cgacgaataa tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag
    11161 accgacggca taggtctgcc aaccttttgc ttcaaatccc agtaacccca ccgcgccatt
    11221 aagaatgaat ataaggccaa tggcgaacgc atagtagtgc cagtcacggc ggatttttac
    11281 aggcagattc atggcagctc cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac
    11341 cgtctgtggt gtagacaaca ttttcgtaat tgttaataaa tttaacttaa ggttgccatt
//
LOCUS       DAJTSL010000130        11299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTSL010000130 DAJTSL010000000
VERSION     DAJTSL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.130"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            292..1269
                     /gene="csiD"
                     /locus_tag="OO433_003119"
     CDS             292..1269
                     /gene="csiD"
                     /locus_tag="OO433_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6051506.1"
                     /translation="MNALIAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKPLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1289..2557
                     /gene="lhgO"
                     /locus_tag="OO433_003120"
     CDS             1289..2557
                     /gene="lhgO"
                     /locus_tag="OO433_003120"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6051507.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFNDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRATRSVDALHAAFNQ"
     gene            2580..4015
                     /gene="gabD"
                     /locus_tag="OO433_003121"
                     /pseudo
     CDS             2580..4015
                     /gene="gabD"
                     /locus_tag="OO433_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            4029..5295
                     /gene="gabT"
                     /locus_tag="OO433_003122"
                     /pseudo
     CDS             4029..5295
                     /gene="gabT"
                     /locus_tag="OO433_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            5533..6894
                     /gene="gabP"
                     /locus_tag="OO433_003123"
     CDS             5533..6894
                     /gene="gabP"
                     /locus_tag="OO433_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6051508.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYVPVKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTDLLAIGIICTVPIMAA
                     GIS"
     gene            6953..7615
                     /gene="csiR"
                     /locus_tag="OO433_003124"
     CDS             6953..7615
                     /gene="csiR"
                     /locus_tag="OO433_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6051509.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7616..8065)
                     /gene="kbp"
                     /locus_tag="OO433_003125"
     CDS             complement(7616..8065)
                     /gene="kbp"
                     /locus_tag="OO433_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6051510.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8149..8307)
                     /locus_tag="OO433_003126"
     CDS             complement(8149..8307)
                     /locus_tag="OO433_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6051511.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8490..8789
                     /locus_tag="OO433_003127"
     CDS             8490..8789
                     /locus_tag="OO433_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6051512.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8799..9323
                     /gene="ygaP"
                     /locus_tag="OO433_003128"
     CDS             8799..9323
                     /gene="ygaP"
                     /locus_tag="OO433_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6051513.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDRIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9370..9774)
                     /gene="stpA"
                     /locus_tag="OO433_003129"
     CDS             complement(9370..9774)
                     /gene="stpA"
                     /locus_tag="OO433_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6051514.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            10442..10891
                     /gene="alaE"
                     /locus_tag="OO433_003130"
     CDS             10442..10891
                     /gene="alaE"
                     /locus_tag="OO433_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6051515.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGIYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(10928..11272)
                     /locus_tag="OO433_003131"
     CDS             complement(10928..11272)
                     /locus_tag="OO433_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6051516.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
BASE COUNT         2666 a         2857 c         2981 g         2795 t
ORIGIN      
        1 ttagtttact aacggtcatt ttgcagtgaa gccatttact gttttttatc gaccagataa
       61 tctgttctct aatgttaact ccccctaacc tgttgcttta gttattcatt tcctgtctca
      121 ctttgcctta ataccctacg ttaaatgtta ctaatttgtt gtttttgatc acaataagaa
      181 aacaatatgt cgcttttgtg cgcatttttc agaaatgtag atatttttag attatggcta
      241 cgaaatgagc atcgccatgt caccctacat ctcataagag gatcgcttct gatgaatgca
      301 ctgatcgccg tacaaaataa cgctgtcgat tcaggccagg actatagcgg attcactctc
      361 atcccgtcgg cgcaatcccc gcgtctgctg gaactcacct tcaccgaaca gacgaccaaa
      421 cagtttctcg aacaggttgc cgaatggccc gtgcaggcgc tggagtacaa atcgtttctg
      481 cgttttcggg taggcaaaat tcttgacgat ctgtgtgcga atcagctgca accattgctg
      541 ttgaagcccc tgctaaaccg tgctgaaggt gcgctgttga tcaatgcggt gggtatcgat
      601 gatgtggcgc aggcagatga gatggtgaag ctggcaacgg cggtagcgca tctgattggt
      661 cgctcaaatt tcgatgcgat gagcggtcag tattacgcgc gtttcgtggt gaaaaatgtc
      721 gataactcag acagctatct gcgtcagccg caccgcgtga tggaactgca caacgacggc
      781 acgtatgtcg aagagatcac cgattacgtg ctgatgatga aaatcgatga gcaaaacatg
      841 cagggtggaa attcgttgct gctgcatctc gatgactggg aacatctgga ccactatttc
      901 cgccatccac tggcgcgtcg cccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc
      961 aaagatgttt tccatccagt gttcgacgtt gatcaacagg gtcgcccggt gatgcgctat
     1021 atcgaccagt tcgtccagcc aaaagatttt gaagaaggcg tgtggttgag cgagctttcc
     1081 gatgccattg aaaccagcaa aggcattctt tctgtacccg ttcccgttgg caaattcctg
     1141 ttgataaaca acctgttctg gctgcacggt cgcgaccgct ttactccgca cccggatctg
     1201 cgccgtgaac tgatgcgtca gcgtggctat ttcgcttacg ccactcacca ctaccagacg
     1261 catcagtaag cgcaaaggaa ttgagcggat gtatgatttt gtgattattg gcggcggcat
     1321 catcggcatg tcgaccgcca tgcaactgat tgatgtttat ccggatgccc gcattgcgtt
     1381 gctggaaaaa gagtccggcc cggcctgtca ccagacgggc cacaacagcg gcgtgatcca
     1441 tgccggggtc tattacacgc ccggcagcct gaaggcgcag ttttgcctgg cgggaaaccg
     1501 cgccaccaaa gccttttgcg atcaaaacga cattcgctat gacaactgcg gcaagatgct
     1561 gatcgccacg tccgatctcg aaatggaacg gatgcgcgcg ttgtgggaac gcacggcggc
     1621 gaacggtatc gagcgcgagt ggttaaacgc cgaggaactg cgcgagcgcg aaccgaatat
     1681 caccgggctc ggcggcattt ttgtgccgtc cagcggcatt gtcagctatc gcgaagtcac
     1741 agcggcaatg gcaaaaatct tccaggccag aggcggtgag attatttata acgccgaagt
     1801 cagcgccctc agtgagcata aaaacggcgt ggtcgtccga acccgtcagg gcggcgaata
     1861 tgaagcatcg acgctgatta gctgttccgg gctgatggct aaccggctgg tgaaaatgct
     1921 cggcctcgaa ccgggtttta ttatctgccc gttccgtggc gagtatttcc gccttgcgcc
     1981 ggagcataac cagattgtta accatctgat ttaccccatc cctgacccgg caatgccgtt
     2041 tttgggcgtg catctcaccc gcatgatcga cggcagcgtg accgtcgggc caaacgcggt
     2101 gctggctttt aaacgcgaag gctatcgcaa gcgcgacttc tcatttaacg acacgctgga
     2161 aattttaggc tcgtcgggga ttcgccgggt gctgcaaaac catctacgct caggactggg
     2221 cgagatgaaa aactcgctgt gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc
     2281 ccggctttcg ttaagcgatc tccagccgtg gcccgccggt gtgcgggcgc aggcggtatc
     2341 gccggacggc aagctgattg acgattttct gtttgtcacc accccgcgca cgatccacac
     2401 ctgcaatgcg ccctccccgg cagcgacatc agcaattcct attggtgcgc atattgtcag
     2461 caaggtacaa acgctgttgg caagccagag taaccccgga cgcacgctgc gagcgacacg
     2521 tagtgtggat gccttacacg ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa
     2581 tgaaacttaa cgacagtaac ttattccgcc agcaggcgtt gattaacggg gaatggctgg
     2641 acgccaacaa tggcgggatc atcgacgtca ccaatccggc gaacggcgac aagctgggta
     2701 gcgtgccgaa aatgggcgct gatgaaaccc gcgccgctat cgacgccgcc aaccgcgccc
     2761 tgcccgccag gcgtgcgctc accgccaaag aacgcgccaa cattctgcgc aactggttca
     2821 atttgatgat ggagcatcag gacgatttag cgcgcctgat gaccctcgaa cagggcaaac
     2881 cactggccga agcgaaaggc gaaatcagct acgctgcctc ttttattgag tggtttgccg
     2941 aagaaggcaa acgtatttat ggcgatacca ttcccggtca tcaggccgat aagcgcctga
     3001 ttgttatcaa gcagccgatt ggcgttaccg cagctatcac gccgtggaac ttcccggcgg
     3061 cgatgattac ccgtaaagcc ggtccggcgc tggcggcagg ctgcaccatg gtgctgaagc
     3121 ccgccagtca gacaccgttc tctgcgctgg cgctggcgga gctggcgatt cgcgcgggca
     3181 ttgcggctgg cgtgtttaac gtggtcaccg gttcggcagg cgcggtcggt aatgaactga
     3241 ccagcaaccc gctggtacgc aaactgtcgt ttaccggttc gaccgaaatt ggccgacagt
     3301 taatggaaca gtgcgcgaaa gacatcaaaa aagtgtcgct ggagctgggc ggcaacgcgc
     3361 cgtttatcgt ctttgacgat gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga
     3421 aattccgcaa cgccgggcaa acctgcgtct gcgccaaccg tttggcgtgt atgaccgctt
     3481 tgccgaaaaa ttgcagcagg cggtgagcaa actgcacatc ggcgacgggc tggataaagg
     3541 cgtcaccatc gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc
     3601 cgatgcgctg gagaaaggtg cgcgtgtggt ttgcggcgct aaagcggatg aacgcggcgg
     3661 caacttcttc cagccgacca ttctggtgga cgtgccggcc aacgccaaag tgtcgaaaga
     3721 agagacgttc ggccccctcg ccccgctgtt ccgctttaaa gatgaagccg atgtgattgc
     3781 gcaagccaat gacaccgagt ttggccttgc cgcctatttc tacgcccgtg atttaagccg
     3841 cgtcttccgc gtgggcgaag cgctggagta cggcatcgtc ggcatcaata ccggcattat
     3901 ttccaatgaa gtggccccgt tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc
     3961 gaagtatggc atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga
     4021 gaatgcgaat gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg
     4081 gcgttgggca aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg
     4141 ttgaaggccg tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc
     4201 tgcatccgaa ggtggtggcc gcggtggaag cgcagttgaa aaaactgtcg cacacctgct
     4261 tccaagtgct ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc
     4321 cgggcgattt cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg
     4381 cggtaaaaat cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgcgt
     4441 atcacgggcg cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg
     4501 gcatggggct gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca
     4561 taagcgagga tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg
     4621 aagatatcgc cgccatcgtg attgagccgg ttcagggcga aggcggtttc tacgccgcgt
     4681 cgccagcctt tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg
     4741 ccgatgaagt gcagagcggc gcggggcgta ccggcacgct gtttgcggtg gagcagatgg
     4801 gcgtggcgcc ggatcttacc acctttgcga aatcgatcgc tggcgggcgt caccgggcgc
     4861 gcggaagtga tggacgctgt tgcaccgggc ggtctgggcg gcacctatgc cggtaacccg
     4921 attgcctgcg tggctgcgct ggaagtgttg aaggtgttcg agcaggaaaa tctgctgcaa
     4981 aaagccaacg atctggggca gaagctaaaa gacggattgt tggcgatagc cgaaaaacac
     5041 ccggagatcg gcgacgtacg cgggctggga gcgatgattg ccatcgagct gtttgaagat
     5101 ggcgatcaca gcaaaccgga cgcaaaactc accgccggga tcgtggcacg cgcccgcgat
     5161 aagggcctga ttcttctctc ctgcggcccg tattacaacg tgctgcgcat ccttgtactg
     5221 ctcaccattg aagacgctca gatccgtcag ggtctggaga ttatcggtca gtgttttgat
     5281 gaggcgaagc agtagcaccg tccccatgca ggaggcgacg ctgcgcgtct tgtccggcct
     5341 acgagaatcc aggtcggata aggcgtttac gccgcatccg acaatctgta cgtgaacagg
     5401 aataaatcta tgtcggccgg ataatgcgga gccgctctcc ggcaaaaaga atcaataaca
     5461 attaaacgcg tgacccggcg cgggaaatgt cggggcgctc tcccaagtga cacactttcg
     5521 agaggattca ggatggggca atcatcgcaa ccacatgagt taggcggcgg gctgaaatca
     5581 cgccacgtca ccatgttgtc tattgccggt gttatcggcg caagtctgtt tgtcggttcc
     5641 agcgtcgcca tcgccgaagc gggcccggca gtattactgg cctatctgtt cgccgggcta
     5701 ctggtggtta tgattatgcg gatgttggcg gaaatggcgg ttgccacgcc cgataccggt
     5761 tcgttttcca cctatgccga taaagccatt ggtcgctggg cgggctatac catcggctgg
     5821 ttgtactggt ggttttgggt actggttatc ccgctggaag ccaacatcgc cgccatgatc
     5881 ctgcactcgt gggttccagg cattcccatc tggttatttt ccctcgtcat tacccttgcc
     5941 ttaactggca gtaatttatt aagcgttaaa aactacggcg aatttgagtt ctggctggcg
     6001 ctgtgcaaag tcatcgctat cctggccttt attttccttg gtgcagtcgc aattagcggt
     6061 ttttaccctt atgccgaagt gagcgggatc tcaagattgt gggatagcgg cggctttatg
     6121 cccaacggct tcggtgcggt attaagcgcg atgttgatca ccatgttctc gtttatgggc
     6181 gcagaaattg tcaccattgc cgccgcggaa tccgacacgc cggaaaaaca tattgtccgc
     6241 gccactaact cggttatctg gcgtatttct atcttctatt tgtgctctat ttttgtcgtc
     6301 gtggcattaa ttccgtggaa tatgcccgga ctaaaagccg tcggttctta tcgctcggta
     6361 ctggaattgc tcaatattcc ccatgcgaaa ttaatcatgg actgtgtgat attactttcc
     6421 gtaaccagct gcctgaactc ggcgctgtat accgcttcca ggatgctcta ctccttaagc
     6481 cgtcgcggag atgcgcctgc ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgtg
     6541 gcggtgttac tctccaccgg cgcggcattc ttaacggtgg tggtgaacta ttacgtgcct
     6601 gtgaaggtat ttaaatttct gatcgacagc tccggcgcta tcgccctgct ggtttattta
     6661 gtcatcgccg tttcacagtt gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt
     6721 cgcttgcgca tgtggcttta tccctggctc acctggctgg tcatcggctt tattaccttt
     6781 gtgttggtag tgatgctatt ccgcccggcg caacagttag aagtgatctc caccgactta
     6841 ttagcaatag ggattatctg taccgtgcca attatggcgg ctggaataag ctgataatgt
     6901 ggcaaaaaac acccgttcat aatacgcgct gataataatc aggagtcaca ccatgaccat
     6961 tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc
     7021 ggatgaaaaa ttacggatga gtttgctgac atcgcgttat gcacttggcg ttgggccgtt
     7081 gcgggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg
     7141 gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgatg cccgcgccaa
     7201 tatggaagcg atgttagtga gtctggcgat tgcccgcggt agcgatgagt gggaggcaga
     7261 cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat
     7321 gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg
     7381 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gctatcgatt
     7441 tatctggctg cggcgaacgg tgctttcggt agaaatgctg gaggataaac acgatcagca
     7501 ccagaccctg actgcggcgg tactggcgcg agataccgcg cgcgccagtg agttaatgcg
     7561 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
     7621 cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt
     7681 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
     7741 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg
     7801 ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt
     7861 tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt
     7921 gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct
     7981 tcgcctgatc gtctttatcg tgctgacctg taaccgcgtc ccagagtttt tctccggcat
     8041 ctttcacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
     8101 tgtagcagag gaacgcggcg cgttaacgcc gcggggggga gcacaggttt aatcgcgggt
     8161 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt
     8221 aataatgaac gcccaaccga accctttacc gagcagcacg ccgagcggcg gcagaatgat
     8281 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
     8341 aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag tttcagctta
     8401 cgcttttact ctcatttacg ctgctgaggc tggtatatgt tgcattttta gtttagagtt
     8461 tacttaattt agaaaatact taaataaata tgactgaact cgcgcaatta caggccagtg
     8521 ccgaacaggc agcggcctta ttgaaagcaa tgagccatcc taaacgattg ctgattctgt
     8581 gcatgcttag cggttccccc ggcaccagcg cgggagagct gacgcgcatt accggactga
     8641 gtgcctctgc gacatcacag catctcgctc gtatgcggga cgaagggctt atcgacagcc
     8701 aacgggatgc ccaacgcatt ctatattcca ttaaaaatga ggcggtaaat gccattatcg
     8761 ccaccctgaa aaatgtctat tgtccgtaag gagtcactat ggctttgaca accatttcgc
     8821 cgcatgatgc acaagaatta atcgcacgcg gcgcaaagtt aatcgatatt cgtgacgctg
     8881 atgaatatct tcgtgaacat attcctgaag cagatctggc tccattatcc gtgctggaac
     8941 agtcaggtct tccacctaaa ttacgtcgcg agcaaattat attccactgc caggcaggta
     9001 aacgcaccag taataacgcc gataaattag cagcgattgc cgcccccgca gaaatatttt
     9061 tactcgaaga taggattgat ggctggaaaa gagctggatt gccagtagcg gtaaataaat
     9121 ctcaaccctt gccgttaatg cgccaggtgc agatcgctgc gggtggttta atattaatcg
     9181 gcgttgtact gggttatacc gtaaatagcg gtttcttctt attaagtggc tttgttggtg
     9241 ccgggttact gtttgcagga atcagcggtt tttgtggaat ggcaaggttg ttagataaga
     9301 tgccgtggaa ccaacgagct tgagaagcga cgccggacgc gccctagcag cgacatccgg
     9361 cctcagtaat tagatcagga aatcgtcgag agatttacct tctgccagcg cctgagcaat
     9421 tggcttcggt gtacggccct gaccggtcca ggtcttagtt tcaccgttaa catcagtgaa
     9481 tttatatttc gccggacgcg gctggcgttt tttaccagca cgtggtgcag cagcagagct
     9541 attacccaat aactcttccg ggttaattcc gtcagctttc atcagctcca gccaggtgct
     9601 aattttttcc tgacgctcag ccagttcacg ctgctgctgt tcttcttctt cacgtctttc
     9661 tttagtgaca accctgaatt tttcgagcat ttcttcaaga acgtcaatgg agaattcgcg
     9721 agccatcgca cggagggttc gaatgttatt taaactttgt aacattacgg acataataaa
     9781 gaaaaccttt taacgccaaa acaaaaaagt atttcgtcgc gtagtgtaag cgatcactta
     9841 ttatatttcc acaaaattta atctatttac agtcctgcgc gagattattt cagctgttat
     9901 tttctgaaac tctcagaata atttcaagat tatatattag ccattttgat gagtaattta
     9961 ggttattggt gaacagagtg gcgctctctg ctcgctaact ccgcaagcct gataaaaccg
    10021 aagtgaataa aaatgcaata taacagaata aaagaaaaca cccctcactg gtgctttctg
    10081 ctgttaatgt acattttctg gcatcaaaaa tgctgtaaat gacccggtca cgacaaacac
    10141 cgaattctct acgcttatag cgttacctca cccccaaact ttcattagtg aaattaaagg
    10201 caaggtcata tccaattgat ttaattgaaa ataaatcata tcggcatgta aaaatccaca
    10261 atgtacaaaa aacgagctgt tacggaatat tttatctaca aaaaactgac taaataaaag
    10321 tttttcacta attgattagt catagccagc gatatacgct atgcgaaaat gcagatggca
    10381 atgagatcca ctgctttcat ctccattaac atcccattac gctttattaa ggagcattag
    10441 catgttctca ccgcagtcac gcttgcgtca tgcagttgca gatacgttcg cgatggttgt
    10501 ttactgttct gtcgtgaaca tgtgtattga agttttcctc tccggaatga gcttcgaaca
    10561 gtctttttat tccagattgg tagcgattcc ggtgaacatc ttaattgcat ggccatacgg
    10621 tatataccgc gatctgttta tgcgcgcggc acgcaaagtt agcccgtcgg gctggataaa
    10681 aaatctggct gatatcctgg cttatgtgac gttccagtca ccggtgtatg tggcgatctt
    10741 gttagtggtg ggcgcagact ggcatcagat tatggcggcg gtcagttcaa acatcgttgt
    10801 ttcgatgttg atgggggcgg tttatggcta cttcctcgat tattgccgcc gactgtttaa
    10861 agtcagccgt taccagcagg taaaagcctg actcttcttt tcgcgactgg catcgccagt
    10921 cgcgtcttta actggcttcg ccaaataaac cattcaaata acgttcaagc gcaacacgcg
    10981 aactgaagcc atgcgggata ccgtaatgct cgtgtcgctc gcctgctaaa tagagcggaa
    11041 attgctggta atgatcgcaa gttttaatat cggaagcggg ttcagccagt gttgaactgc
    11101 gttcttttaa tgttggatga attaccagag cggtacgtcc cattcgcgct tcgcgattaa
    11161 catagacata attttcccca cggcggtaac catacgcttt ctgggttacc acatcgacag
    11221 taaaaccgac tttttcaagt acgcgcgcca cctcgtctgg tcgtaaatac atatttgatc
    11281 ctcgttattt tcaaccgcg
//
LOCUS       DAJTSL010000131        11226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTSL010000131 DAJTSL010000000
VERSION     DAJTSL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.131"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            63..661
                     /locus_tag="OO433_003132"
                     /pseudo
     CDS             63..661
                     /locus_tag="OO433_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(690..1178)
                     /locus_tag="OO433_003133"
     CDS             complement(690..1178)
                     /locus_tag="OO433_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6051517.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(1191..3998)
                     /locus_tag="OO433_003134"
     CDS             complement(1191..3998)
                     /locus_tag="OO433_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6051518.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVAFGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGCFYDKD"
     gene            complement(4036..4140)
                     /locus_tag="OO433_003135"
     CDS             complement(4036..4140)
                     /locus_tag="OO433_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCR6051519.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVS"
     gene            complement(4149..4523)
                     /locus_tag="OO433_003136"
     CDS             complement(4149..4523)
                     /locus_tag="OO433_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6051520.1"
                     /translation="MKDIDTETRNNTVADNVTAVEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            complement(4579..5089)
                     /locus_tag="OO433_003137"
                     /pseudo
     CDS             complement(4579..5089)
                     /locus_tag="OO433_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
     gene            complement(5089..6273)
                     /locus_tag="OO433_003138"
     CDS             complement(5089..6273)
                     /locus_tag="OO433_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HCR6051521.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIADKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKNGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            6431..7540
                     /locus_tag="OO433_003139"
                     /pseudo
     CDS             6431..7540
                     /locus_tag="OO433_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            7789..10908
                     /locus_tag="OO433_003140"
     CDS             7789..10908
                     /locus_tag="OO433_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6051522.1"
                     /translation="MIGQFLSATEILAKNYVRNKMVKNPFYSNLKWNFIEKNIIRLTS
                     SPVKSVLCISAFSFVLLYVGYLNELFIKNNLLHYFPFRHSLTEWQTTILSGQLTIIGI
                     VYPLVIGLVSVLFQKKADRKIAQTAYQRYSGFMLAGLSGLFLSGFILLSVLIKTVFGS
                     YLYGIACLISILWLLINIVLSIWFFIVSLEILDDVKRQIIIKRYIAFEIVMPHICNKI
                     SAKLRLYPIYQKHNYSNLEITQADYKGEYISVASSYSKEDELSLYHRPFQLTLNLINY
                     QLKKKNHFASFVIGDNRTKETESTGKILFSVKNIKPDSLLIKILKQCFYRAPIKGGDF
                     SVSLTMQAITADTYMYLRDSDLISFDNAISALINNFNNLCDLYFFQDDNTNNNFLLIT
                     TELFERSFQYEFSDEVYKISNNSMDKINLSERFFELCLWSGVRIINNRKHLISNELCI
                     YMGITRSQWSILTEWFRNNQSLLNASLRSRYNRILRTYITVWEQYQESINFRFCNTEN
                     SDLFELFCKTQLQELPSMIIDATQTRDPSTIDTAVDLINRWQHSMNIDSHSVEKYSYK
                     GQLFNPGFFISKKLNFNSDREWFNIAIINALTDMRICTCLYLTSRINTSDKLMTHYIK
                     LILEGKLIDQTGGYETPTEEIDNASQLIKILIRICLWTWSENMEHNGWMNSLARRLRD
                     YDKTDMVMGRVYSNVFDCGFIDMEQSWVQLLLIFSNKNDSVSKEIKEAIENNYITYRE
                     KQRLIGVLSKICNSIEYTKIKLTLTLDDLQTKKENLRKLLQEHINMLKKDLDMRLQDA
                     AIDVHRLDSTARKTSEHLRKRIKKTLPLSLFKSIDFKQASDCFTKHKISIKIDKEPYA
                     EGIESIPYINEGDIQADLILKDIQRIILSNLFSTGCSQHTVIEDFNMLIDHIKSSADL
                     AGKLVLVMSKEIFQQYNRMLFDNPNLRELMRKNDDGSMNITTESGTRKVYFLPFVNQP
                     FSLVVKDNYFTKLIIREYDNNKLVNVTSENIKSDSDKFKLTLNYELNIVFEGNADLKI
                     AHSQRVTSE"
     gene            11044..>11226
                     /locus_tag="OO433_003141"
     CDS             11044..>11226
                     /locus_tag="OO433_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR6051523.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASPIHSS"
BASE COUNT         3074 a         2814 c         2511 g         2827 t
ORIGIN      
        1 ttaaataatg gccgtttctg gcctgcatgg catgatttgc gcttttggac gggagattca
       61 gtgtgctgat tggctatgta agggtatcaa caaatgacca gaatacagac ctgcaacgaa
      121 acgctcttgt ttgtgcagga tgtgaacaaa tatttgaaga taaattaagc gggacaaaga
      181 cagaccgacc gggattaaaa cgcgctttaa agcgccttca aaaggtgaca cgctggttgt
      241 ctggaaactg gatcgcctcg ggcgaagcat gaaacatctg atttctctcg tcggggaact
      301 acgggagcga gggattaatt ttcgcagtct gaccgacagc atagacacat cttctccaat
      361 ggggcgtttt ttcttccacg tgatgggtgc cctggctgaa atggaacgtg aattaattgt
      421 tgaacgtaca ctggccgggc tggcggcagc gcgcgcaagg gggcgcacag gcggacgtcg
      481 accgaagctg acaaaagaac agcatgagca aatagcaagg ctaatcaaaa acgggcacga
      541 cagaaaacaa ctggcaataa tttacggcat tggcacatcg acgatttatc gttatcaccc
      601 tgtaggcgat atacagactg aagaaacaac cgggcagact caggaaaatg aaaaccgcta
      661 atctgaccat tagcggtttt tgtgttaaat cagaacagcc ctttaactga actggccgcg
      721 ctgttaagag atgatgtcac cttatctttg aagccggaca gcatatcact gaacgatgag
      781 gattgcaggc gctcccgcaa atcctcatca cagcgttcaa gagtcagtga aaattctatc
      841 tttttcgcct taccgtagcg atcaaactcg gagcgggtcg tattcgtttc agtcaggaca
      901 tacatgccgt aaatctgccc gacgccatca atcaaaggcc agggtcgtcc tgtatacgcc
      961 tgcgtagtca gcagcgacag cgacacttcg ccacctgtaa tttcaggata aagcacacca
     1021 gaaagaacga tgcgatcatc acctgcaccg atatactgcc agcttgctga acggttaacg
     1081 cgttcatttt tcacatgccg ccagcttttg ttttgctgta actgctgatg cggcagcgtg
     1141 cgcagctcaa aaacaaacat gccgtagatc atcatcatgg ccatgactcc tcaatcttta
     1201 tcgtaaaaac agccacgccc ggcacgggcg cgccgttcca tttctgccct gaccatttca
     1261 ccgaccagtt tcgccagttc gcggggattc tgcgtaacaa cgttatgcag atgaacatga
     1321 atttcaccac caaatccgaa ggcaacaggc tcccggttac gggaagttac aggaactgat
     1381 gccactggag atcgtatagc ctccgccacc gggcgggagc tggccgcaac aacagggacc
     1441 agcgccggag gcagcggagc cggaaccacg ggtgtgatat taattgcggg ggcaggctta
     1501 ctgacctgcg caatcttccg ctcctgccac tccccacgaa cagcaagtgc tcggggcagg
     1561 tttttaaaga caatatcgcc ggggccaatg cgttttttcg tctcctcaac cagcttacct
     1621 gtgttatcag caattttgct gagtctgcgc agcgtaccgg tattgctgtc tgtgagcggt
     1681 ttattgtctt tggggttatc acctccggtg ccattgccat tttccacagg cttcggcgga
     1741 ttgattttcg ccagatcccc ctgaagcaag gcaaccttgt cctgaagaat ggccgcacgc
     1801 tgtgcgtctt cgattttctt gcgcgccctt tccgcttcat ccggaagcac accaagcttt
     1861 tcaagtatcc acgccagcgt atccagcaac atttttgcag gtgtcagaac aagttgtaac
     1921 gcaccgccaa gaacgttacc gaatatctcg ccagcactgg tacatttatc cagagtttcc
     1981 ttgctggact ccatcggtga cagcagcgat ttaaaccagt taaacacctg gctgatccca
     2041 ctcccgattg cgtcaaaaac aggaccaaac cgttcaaagg tttcgcgcaa cggggtcagc
     2101 ctttccataa tcccgctgaa caccccggca aaaaatgccc tgatgggatc ccagtatttc
     2161 cagataagaa cggcagctcc ggcaagcgca gccacgataa gaccaaccgg actgaacaac
     2221 gccccgatag cgcctcccag taaagaaacg gaacccgtca ccattcccca tagtgctggc
     2281 agaaccctga cagcattcat tgatccggtc aggagggaaa aaccaagacg cagttttgcc
     2341 agcgggccag caagcacacc aatagccagc gacaacgagc caaccgttgc agtcattgcc
     2401 agcaatgcac cgcctgcaat cagtagctgg cgcgtcagtg cgggatgggc ctgcgccagc
     2461 gccgtcacct ttgataccac acgcgtgagc cactgcgtga cagaacgcag cggaccgtca
     2521 atcagatctg caatgcggat gcgcaaccct tcccatgcac tgctgagtga tttcagatcg
     2581 ccgtcaaggt tgttggccat aacctttgcc gtgcgttcag cctcaccgcg cgcgccttca
     2641 agttcttttc tcagtttggg taaggagccg tcacctgccg catcaacgag ggccataaat
     2701 gatgtgaaag cctcttctcc ggcaatgtcc ttaaagaacg atacccggtc aacttccccg
     2761 tatttgcggg tagctttata aaggtcagcc agcacatcct ccatcgggcg cattttgccc
     2821 ccggcatccg agacagacac gccaagctct ttcagcgcct ctgctgccgc ctttggcggt
     2881 gatgccagac gagccaggct ggcacgcatt gccgtaccag catcactccc cctgataccc
     2941 atattcgcca gcacgcccgc catcgctgcg gcctgctcca gagatattcc cagcttaccc
     3001 gccaccggac ctgcatattt catggtttca cccagtgcgc gaaggtcagt gttggtacgg
     3061 gtaaacgctg cggtgagtgt gtcgccgacc cggtccatct ggtcagcaga aagaccgaac
     3121 tgcgtcagaa tatttgagcc aatatccgcc gtctcaccga ggtccatacc gccagccgtt
     3181 gccatgctca gtacgccagg gagcgcagcc tgaatggcct gtggtgtaaa gccagccatt
     3241 gcaagaaatg cctgtccact ggcggcatcg cctgcggtga actgcgtttc agagccaagt
     3301 tttaacgcct gctcacgcag cgccttaaac tgcgggctgt tctggtcgat tcgcgtcagt
     3361 gcctgaacgc gggacatctc tttgccgaac ccgatcgcag gctgcaaaaa acgcccggca
     3421 gcatagccgc ccgccgctgc cgcaccaatt gccagcgcac cacctgtttt cagttttccc
     3481 gctgtttcct gcgcgcgcga ataccgctca cgcgcccgtg ttacacgcgc aagcgcctgc
     3541 cgttcgcgtt caagctggtt gttgtactgt tcggtgcgtc tgatggcctg ctggatggtg
     3601 ttatcgctgc ctgtcaggga aatgccgtgg cgtttcagct ctccgccaag ctcccgcatt
     3661 ttctgaattt cccgtgtgcg cgattcattc aggcgttcaa gccgggtgct taactgctgc
     3721 atcagctttt gttgtttttc gctgagcact gtacccgtgc gttgtaactg attaagggcg
     3781 ttaagctggc gtcgtgcttt cacgatgcca gcatccgctt tactgacagc gtcacgggcg
     3841 cgctcaaatg atcgcgcctg acgctcgaga tttttgatcg ccccctgcgt tcgctggatg
     3901 gagtcaccaa actgccccat caggcggcgg gcgttttcgg caggccgggt cagcctgtca
     3961 acggcgctga aagcgacccg gatatcaaga gtcttcattg tctgcattcc cgctacgaag
     4021 tgccgcccgc tcacgtcagc taaccacttc gccgggcgtc atcatgaaga tttcggcggg
     4081 cgaccagtta aaaatggcgg caatatccgc caccagatct tcgatgtgct caaagcacac
     4141 cagggtgatt acgctgccgt ctcctgcacg ctcttcgcgc cagagtctgg ctcgctcata
     4201 aaatttacag ccacagcgca caactgaata aaatcgcgtg acgacatttt tttaatcatc
     4261 acttcatcca gtcgtggcga ggtcacgcga ggcaacagcg tgaacatggt atccgctttc
     4321 agattcagca catcagacag cgacagacca cgcagggatc cagcctgctc aatagccccg
     4381 gtgatctcca catacgtgat tttttcgcca ccacgctcaa ttggtcgggt cagttttacg
     4441 ccacgttcga cagccatatc ctcaactgcc gtcacattat ccgccacggt gttattccgg
     4501 gtttcagtat cgatgtcttt catctgttgt ctccttttca gtcagaggcg acgcactgcg
     4561 ccgccagcat attacttatc agccaagccc aagcgcggaa cggatgcgat cgggcacaat
     4621 gtccttgccg tccttccggt aaatgaagtt cagcaggtca atctcccaca acgggcgatc
     4681 gttaacactc agcttgtagt aggtgtttta atggcgtaag tgtgtgatgt ggcttcgccc
     4741 tgtttggctt cccccatatc aatttccgtc acacgtccgc gcatttcgac ttcatacagg
     4801 tcgctttctg catcggtgta gtattcaccc gcaaaacgca gcagcgtgcc gtcaatcgtg
     4861 ccgccatact taaggaacag ctcacgaact gcgcccccat gacaaagctc gcatcaagcg
     4921 cggagtcgtc cagaccgaga tcaatactta ccgcacccat catgccacca ccccggtagc
     4981 tgtcggtttt gcgcgtcagc ttaggcagag tgacggacgt cacattaccc acttcgtttt
     5041 caccatccac aaacagcgta aaaaagcgaa gatgttttgg cacagccatc aggcacctcc
     5101 cagcaccgca aatgcgggtt caaagtattc atcagtaaac gtctggtaaa gctccatgtc
     5161 ttccagtggc ggaacgggcg tatatttgta gcgaatacgc acacgtccct gacgtaaatc
     5221 cgtggtgctg ttatccacca cgtcatacca gcactccgcg ccaatcagtt tcccggcagt
     5281 caccagtgaa tccagttttg ccctgatggc actgataaca tccttcacat tcgcaggcgt
     5341 cagtggactg tcgatggttt caaactgcgc ttccgcaatt gaatcagcca gcacctgtgc
     5401 ggttcgggta tacacctcaa agatgtaggc gttcgtttcc ggtgtgcggt taccccagaa
     5461 gcggaacccg ttgcgacgaa taatggtcgt gatttctttg ttgttgaggc tgttggcatc
     5521 gctgtcttcg gcctgcaacg accagaacac atgcctcgac atccccagca catttttaac
     5581 cggaacgttg gacagcgatt tgtgccatcc ctgttcatgg tcaatgtacg cacgaaggcc
     5641 gcacgcatag gcaggcgcgg ggaacgtttc gtttttgcca tttttcgggt tgtaggcgat
     5701 gaagtccggc cataagagca tcacctcacg ttcgttgaat ttctggcggt aggtaattgc
     5761 ctcggccatc gtgttacagc catgacatga ggcatacaca aacgcgcgca gtttatctgc
     5821 aatcacgcac agggattttg ttaccgcctc cgtgtccagc tccggcgcgg ccagaatacg
     5881 cggacggtat ccgatgcttt catcctgctc tgcaacaagc agcgcataca tccccgtata
     5941 gctgccgtca tcctcagaac caccgataac cagttgatcc tgcgtctttc cgtcttcttc
     6001 tttgtgttca gccacgcgaa cgacgatcac ctttgtgctc acctggtctg cgatggcctt
     6061 aagcgcacga taaagcgtcc ccgttgtccc gcattttccc agcacgtcat tgacgcgggt
     6121 cagcagtgtg ggcttgttca gcgggaacag cttcgcgtcc gcatcatccg ccgttgccac
     6181 gataccgata acgctggaat caacatcgtt aatcgctgtt accaggtcgg tattctccgt
     6241 aacacgggca ccatgaaaac gagtttcact catagcttca gccccttgta tccgttaaat
     6301 gattcagcaa caatcatcac ccaccacgcg cgtaatctca cccctgcgcc gttctcccgc
     6361 cacggcgaca acaaaaagca gtaaccccct ccgcacgcac atgcgaccat gccgcacagg
     6421 gagggagcag atgaccgaca ccaccatgca attgctcagt cagggcacgg cccccgtgaa
     6481 aatgccggat tttgatattc tctcggaggg taaaacgctg tcaggcgtgg cggagcgcct
     6541 gatgagcctg taactgaccg acaaccgggg atttgaggcg gaccagctca ccatcacgct
     6601 ggatgatgcg gatggtcagt tgcagctacc gccacggggc gcgcgtctga cggttctcat
     6661 tggctggaaa ggggaaccgc tgacagaaaa aggcacttac attgttgatg aaatcgctca
     6721 cgaaggaccg ccggacaggc tgactgtttc agccagaagc gcagattttc gggatgaatt
     6781 taacgttaaa cgtgaggtgt cctggcatga tgtgaccgtt gagcgtgtgg tatccgccat
     6841 cactcatcgg tacggtctga aaccgcaaat cagcgaaatg ctgatggata tcgaaatcga
     6901 ccacgccgac cagaccgaag aaagcgacat gtcctttctt acgcgcatgg cggaaatgct
     6961 gggagcaatc gccacggtaa aaagcggcaa tctgttattc atcatgccag gtggtggcgt
     7021 gaacgcacag gggcagccgt tgccctcgtt cgccattaca cgcagcagcg gcgatcgcca
     7081 tcagttccgc attgctgacc gcgaggcgta tacgggggta cgcgcctact ggcttgatct
     7141 taattacggg aaaaagaaaa aagtcagcgt gaaacgccgc aaaccgccaa aacccaaaaa
     7201 ggagaaaagc agcagtcggg aaggcgacta tatggaaggt gcggaaggca acgtgtttgt
     7261 gttacgcaag acttatcaga acgagcaggc agcaagacgc gcagcggcgg caaagtggca
     7321 gcagctacaa cgcggagccg catcattctc catcacgctg gcgcgtggtc gtgcagaact
     7381 ctatcccgaa atgcatggca cagtgacagg ctttaaaagc gagattgatt atcaggactg
     7441 gactattgca aaagccgagc acaccattga taacagcggc tttaccacgc agcttgaact
     7501 tgaagcaaaa atcccggaat ggatagcaga aacagaataa atctcttaaa aatattattt
     7561 ttgagatgtg cttctacact gacagtaaga acaaaagata atgtatcagc ccactagatg
     7621 tcagaggttg gggcatcaac cagatgctaa caataatacg gatgtttttc ccacatgaca
     7681 gaggaagtca agtataatct tcacagtcca tcccgtccaa gggaatatga ctcaatagta
     7741 aaagttccag tttagttagt agacccacac tttatagaag agtaattcat gattggacaa
     7801 tttttaagtg caacagagat tttagcaaag aactatgttc gtaataaaat ggttaaaaat
     7861 ccattttatt cgaatttaaa atggaatttt attgaaaaaa acatcataag attaacatcc
     7921 tcaccggtta aatcagtact ttgcatatca gctttttcat ttgtactact ttatgttgga
     7981 tatttaaacg aattatttat aaaaaataat cttttacatt atttcccgtt taggcattca
     8041 ttaaccgaat ggcaaacaac cattttaagt ggccaattga caattattgg tattgtatac
     8101 ccccttgtta taggtttagt aagtgtctta ttccaaaaaa aggcagatag aaaaatagcc
     8161 caaactgctt atcagcgtta ctcaggattc atgcttgctg gactcagcgg gcttttctta
     8221 tcaggattta tacttctcag tgtactaatt aaaactgtct ttggaagtta tctttatggt
     8281 atagcttgtc taatcagcat attatggctt ttgataaaca tagttctttc tatttggttc
     8341 tttatcgtga gtcttgaaat tcttgatgat gtaaaacggc aaataattat aaagcgatac
     8401 attgccttcg aaatagtaat gccccatatt tgtaacaaaa tatcagccaa gcttaggtta
     8461 taccctatct atcaaaaaca taactattca aatttagaaa tcacgcaagc cgattacaaa
     8521 ggagaataca tatctgtggc tagcagttat tctaaggaag atgagttaag tttatatcac
     8581 cgtccttttc aacttactct taatcttatt aattaccaac taaaaaagaa gaatcatttt
     8641 gcgtcatttg tcattggtga taatcgtaca aaagaaacag aatccacggg taaaatactt
     8701 tttagcgtta aaaatatcaa accagacagc ttattaataa aaatacttaa gcaatgtttt
     8761 tatagggctc cgattaaagg aggagatttt tccgtaagtc ttacaatgca ggctataaca
     8821 gcagatacgt atatgtatct gcgggattct gatctcatta gttttgacaa tgctatctct
     8881 gcattaatta ataactttaa taatctctgc gatttgtatt tttttcaaga tgataatacc
     8941 aacaataact tcttactgat cactacagaa ttatttgaac ggagttttca atatgagttt
     9001 tcagatgaag tttataaaat atcaaataat tccatggata aaattaatct ttctgaaaga
     9061 ttttttgaat tatgtctttg gagcggagtg cgtattatca ataatagaaa acatcttatc
     9121 agcaatgaac tttgtatcta tatgggaatt actcgttcac aatggtcaat attaacagaa
     9181 tggttccgaa acaaccaatc actcttaaat gcctcgcttc gttcacgtta taatcgtata
     9241 ttaagaacct atatcactgt ttgggagcaa tatcaagaaa gcataaactt tcgtttctgc
     9301 aatacagaga attcagattt atttgaatta ttttgcaaaa cgcaactaca agaacttcca
     9361 tcgatgatta ttgatgcaac acagactcgc gatccttcta ctattgatac agccgtcgat
     9421 cttattaacc gatggcagca ctcaatgaat attgacagcc attcggtaga aaaatacagt
     9481 tacaaaggcc agctttttaa cccgggtttt ttcatatcca aaaaactaaa cttcaattca
     9541 gacagagagt ggttcaatat tgccatcata aatgcgttga ctgacatgcg tatatgcaca
     9601 tgtctttatc tgacatcaag aatcaatact tctgacaaac taatgacaca ttacattaaa
     9661 cttattttag agggtaaact tattgaccaa accggtggat atgaaactcc cactgaagaa
     9721 attgataatg caagccaact aatcaagata ttaattagaa tctgcctgtg gacatggtct
     9781 gaaaatatgg agcacaatgg gtggatgaat agtttagcaa ggcgtttacg tgactatgat
     9841 aaaacagaca tggtgatggg cagagtttac agtaatgttt ttgactgtgg atttattgat
     9901 atggaacaat catgggttca gctattatta attttttcta acaagaacga tagcgtatca
     9961 aaagaaataa aagaagcaat agaaaacaac tatataacat atcgtgagaa gcaacgactc
    10021 attggagtat tatcaaaaat ttgtaactcc atcgaataca ctaaaatcaa gctgacttta
    10081 acattggacg atctccaaac aaaaaaagaa aacttaagaa agttgcttca agagcatatt
    10141 aacatgctaa agaaagatct tgatatgaga cttcaggatg ctgccataga tgtacatcga
    10201 ctagatagca ctgcacgtaa aacatcagaa catcttcgta aacgcatcaa gaaaacgctc
    10261 cctctttcac ttttcaaatc tattgatttt aagcaggcta gcgattgttt cacaaaacat
    10321 aagatatcta ttaagataga taaagaaccg tatgccgaag gaatagaatc catcccttat
    10381 atcaatgaag gtgatattca agcagactta attctcaagg acatccagag aataatactt
    10441 tcaaatttat ttagcactgg ctgttcgcaa cataccgtaa tagaagattt caatatgttg
    10501 attgatcaca taaaatcatc tgctgacttg gctggcaaac tggttcttgt gatgagtaaa
    10561 gagatctttc agcaatataa tcgtatgctt ttcgataatc ccaacctcag agaactaatg
    10621 agaaaaaatg atgatggctc tatgaacata actactgaat ccggtacccg taaggtttac
    10681 ttcttaccat ttgttaatca accattttct cttgtggtaa aggataacta cttcactaaa
    10741 ttaattatta gagaatatga taataataag ttagtaaatg tcacttcaga gaatatcaaa
    10801 agtgactcag ataaattcaa gctaactctt aactatgagt tgaatatagt atttgaagga
    10861 aacgctgatt taaaaatagc tcactcgcaa agagtgacat cagaataata tctttgacgg
    10921 cgctccttag agtgccgttc gtttattaac taatgctaaa aatcattata tagagaaagt
    10981 catgtttaaa tcttagagaa tacaacctag aatagtgtaa gcactacaat aagagaggtc
    11041 gctatgttcc gttgtccgct ttgtggcgca tctgcccgta tccgcaccag tcgtccggaa
    11101 aatgattcaa acaccgtgag acaaaagtat tatcagtgta acaatctaga atgcggcgta
    11161 tgtttctcaa cactggaagc cttccacaaa ttcacttcaa agcacgcctc ccccattcac
    11221 tcatca
//
LOCUS       DAJTSL010000132        11123 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTSL010000132 DAJTSL010000000
VERSION     DAJTSL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.132"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..245
                     /locus_tag="OO433_003142"
                     /pseudo
     CDS             1..245
                     /locus_tag="OO433_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            416..763
                     /locus_tag="OO433_003143"
     CDS             416..763
                     /locus_tag="OO433_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6051524.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            753..1115
                     /locus_tag="OO433_003144"
     CDS             753..1115
                     /locus_tag="OO433_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6051525.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            1112..1609
                     /locus_tag="OO433_003145"
     CDS             1112..1609
                     /locus_tag="OO433_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6051526.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGGENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(1617..2801)
                     /gene="emrD"
                     /locus_tag="OO433_003146"
     CDS             complement(1617..2801)
                     /gene="emrD"
                     /locus_tag="OO433_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6051527.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCVGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(3220..3309)
                     /gene="tisB"
                     /locus_tag="OO433_003147"
     CDS             complement(3220..3309)
                     /gene="tisB"
                     /locus_tag="OO433_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6051528.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            3874..3972
                     /gene="ivbL"
                     /locus_tag="OO433_003148"
     CDS             3874..3972
                     /gene="ivbL"
                     /locus_tag="OO433_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6051529.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            4078..5766
                     /gene="ilvB"
                     /locus_tag="OO433_003149"
     CDS             4078..5766
                     /gene="ilvB"
                     /locus_tag="OO433_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6051530.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQDIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            5770..6060
                     /gene="ilvN"
                     /locus_tag="OO433_003150"
     CDS             5770..6060
                     /gene="ilvN"
                     /locus_tag="OO433_003150"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6051531.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            6134..6724
                     /gene="uhpA"
                     /locus_tag="OO433_003151"
     CDS             6134..6724
                     /gene="uhpA"
                     /locus_tag="OO433_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6051532.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            6724..8226
                     /gene="uhpB"
                     /locus_tag="OO433_003152"
     CDS             6724..8226
                     /gene="uhpB"
                     /locus_tag="OO433_003152"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6051533.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            8236..9555
                     /gene="uhpC"
                     /locus_tag="OO433_003153"
     CDS             8236..9555
                     /gene="uhpC"
                     /locus_tag="OO433_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6051534.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            9693..11084
                     /gene="uhpT"
                     /locus_tag="OO433_003154"
     CDS             9693..11084
                     /gene="uhpT"
                     /locus_tag="OO433_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6051535.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
BASE COUNT         2444 a         2894 c         3144 g         2641 t
ORIGIN      
        1 gagtggcggt caggtgcttg ctgttccgcc agcgttgcgc gtgctgtggt catacagcga
       61 aagaaaatcg cctgtcacaa agtaaattca gatgccaggt atgtggatat acagcgaacg
      121 ccgatgtaaa tggcgctcgt aacattttag cggcggggca cgccgttctt gcctgtggag
      181 agatggtgca gtcaggccgc ccgttgaagc aggaacccac cgaaatgatt caggcgacag
      241 cctgaacgta gcagggatcc tcgtccttca gggcgtggag gatgtcaatt tgcgctatga
      301 tacccatctg acctccttgt agcggatggc aaaagcatat caggaaacgg gcggacgaaa
      361 ttcttaaaaa aaaccgctaa agatagagcg ttagtgaaat tgcaaaggag caacaatgaa
      421 aatttcccgc cttggagaag cgcccgatta ccgcttctcg ctggctaatg agcgtacttt
      481 tctggcgtgg atccgtaccg cactaggatt tctggcggcg ggggttgggc ttgatcaact
      541 tgcgccagat ttcgccacgc ccgtcattcg tgaactgctg gcgttgttgt tgtgtctgtt
      601 ttccggcggg ttagcaatgt atggctacct gcgctggtta cgcaatgaaa aagcgatgcg
      661 tctgaaagag gacctgccct acaccaacag cttattaatc attagcttaa tattgatggt
      721 cgtggcggtg atcgttatgg gactggtgtt gtatgccgga tagccgcaaa gccagacgca
      781 ttgccgaccc aggactgcag ccggagcgca catcactggc gtggtttcgt accatgctgg
      841 gctacggcgc gttgatggcg ttggctatca aacacaactg gcaccaggcg ggcatgttat
      901 tctggatttc gattggcatt ctcgccatcg tggcgctgat cctctggcac tacacccgta
      961 atcgcaattt aatggatgtc acgaatagcg atttttccca atttcacgta gtccgtgaca
     1021 aatttttgat ctccctcgcg gtgttatctc tcgcaatact gtttgctgta acgcatatac
     1081 atcaacttat cgtatttatt gagagagtcg catgacagga agtcaggtca ttgatgctga
     1141 agaggacagg cataaactgg ttgtagagta caaggatgct ctgcaacctg ccgattttta
     1201 tcacaatttc aaacagcggg gcatccgctc cgtacagctc attccctatc ttgaatttga
     1261 tgaccggggc gatctgacgg ccgcttcggt gacagcagag ctgtggggga aatttttaat
     1321 tgccctgttc gagtgttggg tgcgggcaga tatcagtcgt atctcgattg agctttttga
     1381 cgccacctta caaaaatggt gtggcggcga aaacccgcaa ccgcgccgtg actgccaggc
     1441 gtgcgactgg catcgcctgt gcccgcacgc tcggcaggag acgccagaca gcgtgctttg
     1501 tgcgggctat caggcgtttt attcttactc cgcaccgcat atgcgggtga tgcgtgatct
     1561 gataaagcag catcgttctc ccatggagct gatgacgatg ctgcggtgac gtgcgcttaa
     1621 acgggctgcc cctgatgcga catccgcgtc gccagcggca gccagcacag cacgatcaac
     1681 aatcccatta aggtcatcaa taaccccagg ctaccctgac cggtttgcgg caacatcgca
     1741 gagagcgacg ccagcacgcc ggaaccaatg ttttgcagac cgccgaccag cgcgccagcc
     1801 gtgcccgcca ggaaggggaa cggctccatc gcgccgctgg tcgccagcgg aaacagcatc
     1861 ccggcaccga aaaagaacag cgcggcggga acgagcagcg tccagacatt catcacgcca
     1921 aaccagtcgg ggatccacat cagcaagcca gccagcaggc agcagataac cgactgccac
     1981 attaacgtgg agaagcgttt attgggacgt ccggcaaacc atgcgccaaa aaacgctgcc
     2041 ggaatcggca gaataaacaa aatactgacc gtcatactgc tcagccctaa caccgcgccc
     2101 atcagcacgc cggagcaggc ttcaaaggcg gcaatcccgg ccagaccgcc aatcagcatc
     2161 agcaaataac agttaaaacc gctgttaccg aaaagcgttt tataactggt aagcaggcgc
     2221 gtgcgcggtg catcgaccgg acgcgtttcc ggcatccagc gggccatact gaaggtcaca
     2281 ccaacacaaa gaaccaacaa aaagagataa caggcgcgcc agttccacat cgtatccagc
     2341 agaccgccga ttagcggtgc gagcaacgga ctgacgagaa tccccatgtt taacaggctg
     2401 ttagcatggc gcaactgtgt ccgttcatat aaatctcgcg gcaaagtacg cgccattacg
     2461 ccgccaacgc cggtgcccat cccctgcatc gcgctggcgg caatcaatac cgtcaaactg
     2521 gaggtcgtga ccgcgaccag cgttgccagc ataaaaatgg acattccgac gaggatcacc
     2581 ggtcggcgac ccacacggtc ggaaatcggg ccataaaaca gctgtgagac accgtaagtc
     2641 agcagataag cgcccattac gctctgcacc gccccttcac ggacgttgag atcgcgcgcc
     2701 atatcggcaa tagctggaat ataaatggtt tgcgccatct gaccgacggc cacgagtaat
     2761 accaacatca ataacaaatt gacgtttctt tgccttttca ttatcacgga tgcttttata
     2821 aaaataatta gaataagtca ctacgcattc ccataaatgc gggagaggaa tctatcacat
     2881 tgatgaatgt tagccagata tacgccagga atggggaatt gtttagcggc agaatatgta
     2941 aacaaaagcg gcaataaatg ttgtcgggag ggattacgtg cgaactgtaa ccctctcccc
     3001 aaaggggcga gggggaaacc accgttccct ttcagaaaat gagctaaaca ttgcacggga
     3061 cggtcccctc gcccctttgg ggagagggct agggtgaggg gcacaggcag cgcaggtgca
     3121 aacattctgc tggtagaaga agcccccaac tccaacccgc acgctaaata taaaagggga
     3181 gcggttaccc gctccccttt ggtgcgactt gaatctgaat tacttcaggt atttcagaac
     3241 agcatcaagc agttgcagtg ctgcaacaat gagtttgagg ataagaatgg cgatatccac
     3301 caggctcata cgcgtctcct gtggttcagg agcactgcca gctgacgtac ctttccgctg
     3361 cctgttgcca gctgttgtgc acgcggtatc tcaaggagaa acgttgtgct tgttgacata
     3421 acacagtgtg ctcacgcggg gttaaggcgc tgacggcacc acccgtttca gccaggactt
     3481 agtgcgccgg gtaacgatgc ggcccccgca taatacattg cgtacagtga tattatagaa
     3541 gtttactgta taaataaaca gtgatatttg gacaaaacgc aaactgtgat cgatactcaa
     3601 ctatgcccta aatacgtcaa aattcgtgcc gaaattgcgc gttctgcgcg gaacacgtat
     3661 actttcagtg ttgacataat acagtgtgct ttgcggttac cagctgcagg cggctgatga
     3721 aacctcgctc cggcggggtt ttttgttacc tgcaattcag tacaaaacgt gatcagcccc
     3781 tcaattttcc cttgctgaaa aattttccat tgtctcccct gtaaagctgt gcttgtataa
     3841 atattgttaa acacaaaacc aacaaggtcc ccaatgacta cttccatgtt caacgcaaaa
     3901 ctactaccaa ctgcgctatc cgccgcagtg gtcgtcgtgc gtgtggtggt ggtcgtcggc
     3961 aatgcgccgt agggactgga acaacacacg attccaaaac cccgccggcg caaaccgggc
     4021 ggggtttttc gtttaagcac ctcccggaaa gtcggcccag aagaaaagga ctggagcatg
     4081 gcaagttcgg gcacaacatc gacgcgtaag cgctttaccg gcgcagaatt tatcgttcat
     4141 ttcctggaac agcagggcat taagattgtg acgggcattc cgggcggttc tatcctgcct
     4201 gtttacgatg ccttaagcca aagcacgcaa atccgccata ttctggcccg tcatgaacag
     4261 ggcgcgggat ttatcgctca ggggatggcg cgcaccgacg gtaaaccggc ggtctgtatg
     4321 gcctgtagcg gaccgggtgc gactaacctg gtgaccgcca ttgccgatgc gcggctggac
     4381 tccatcccgc tgatttgcat caccggtcag gttcccgcct cgatgatcgg caccgacgcc
     4441 ttccaggaag tggacaccta cggcatctct atccccatca ccaaacacaa ctatctggtc
     4501 agacatatcg aagaactccc gcaggtcatg agcgatgcct tccgcattgc gcaatcaggc
     4561 cgcccaggcc cggtgtggat agacattcct aaggatgtgc aaacggcagt ttttgagatt
     4621 gaaacacagc ccgctatggc agaaaaagcc gccgcccccg cctttagtga agaaagcatt
     4681 cgtgacgcag cggcgatgat taacgctgcc aaacgcccgg tgctttatct gggcggcggt
     4741 gtgatcaatg cgcccgcacg ggtgcgtgaa ctggcggaga aagcgcaact gcctaccacc
     4801 atgactttaa tggcgctggg catgttgcca aaagcgcatc cgttgtcgct gggtatgctg
     4861 gggatgcacg gcgtgcgcag caccaactat attttgcagg aggcggattt gttgatagtg
     4921 ctcggtgcgc gttttgatga ccgggcaatt ggcaaaaccg agcagttctg tccgaatgcc
     4981 aaaattattc atgtcgatat cgatcgcgca gagctgggta aaatcaagca gccgcatgtg
     5041 gcgattcagg cggatgttga tgacgtgctg gcgcaattga tcccgcaggt ggaagcgcaa
     5101 ccgcgtgcag agtggcacca gttggtagcg gatttgcagc gcgaattccc gtgtccaatc
     5161 ccgaaagcgt gcgatccatt aagccattac ggcctgatca acgccgttgc cgcctgtgtc
     5221 gatgacaatg cgattatcac caccgatgtg ggtcagcatc agatgtggac cgcgcaagct
     5281 tatccgctca atcgcccacg ccagtggctg acctccggtg ggctgggcac gatgggtttt
     5341 ggcctgcctg cggcgattgg cgcggcgctg gcgaacccgg atcgcaaagt gttgtgtttc
     5401 tccggcgacg gcagcctgat gatgaatatt caggagatgg cgaccgccag tgaaaatcag
     5461 ctggatgtca aaatcattct gatgaacaac gaagcgctgg ggctggtgca tcagcaacag
     5521 agtctgttct acgagcaagg cgtttttgcc gccacctatc cgggcaaaat caactttatg
     5581 cagattgccg ccggattcgg cctcgaaacc tgtgatttga ataacgaagc cgatccgcag
     5641 gctgcattgc aggatatcat caatcgccct ggcccggcgc tgatccatgt gcgcattgat
     5701 gccgaagaaa aagtttaccc gatggtgccg ccaggtgcgg cgaatactga aatggtgggg
     5761 gaataagcca tgcaaaacac aactcataac aacgtaattc tggagctcac cgttcgcaac
     5821 catccgggcg taatgaccca cgtttgtggc ctttttgccc gccgcgcttt taacgttgaa
     5881 ggcattcttt gtctgccgat tcaggacagc gacaaaagcc atatctggct actggtcaat
     5941 gacgaccagc gtctggagca gatgataagc caaatcgata agctggaaga tgttgtgaaa
     6001 gtgcagcgta atcagtccga tccgacgatg tttaacaaga tcgcggtgtt ttttcagtaa
     6061 ccgctcaagg cttgaacaac atcgcgctta tcgttaaggt aagcgcgttt tttttacccg
     6121 ccaggacaag accatgatca ccgttgccct tatagacgat cacctcatcg tccgctccgg
     6181 ctttgcgcag ctgctggggc tggaacctga tttgcaggta gttgccgagt ttggttcggg
     6241 gcgcgaggcg ctggcggggc tgccggggcg cggtgtgcag gtgtgtattt gcgatatctc
     6301 catgcccgat atctccggtc tggagctgct aagccagctg ccgaaaggta tggcgacgat
     6361 tatgctctcc gttcacgaca gtcctgcgct ggttgagcag gcgcttaacg cgggggcacg
     6421 cggctttctt tccaaacgct gtagcccgga tgaactcatt gctgcggtgc atacggttgc
     6481 cacgggcggc tgttatctga cgccggatat tgccattaaa ctggcatccg gtcgtcagga
     6541 cccgctaacc aaacgtgaac gccaggtggc ggaaaaactg gcgcaaggaa tggcggtgaa
     6601 agagattgcc gccgaactgg gcttgtcacc gaaaacggta cacgtccatc gcgccaatct
     6661 gatggaaaaa ctgggcgtca gtaacgacgt agagctggcg cgccgcatgt ttgatggctg
     6721 gtgatgaaga cgttgttctc ccgcttaatt accgttattg cctgcttttt tatcttctct
     6781 gccgcatggt tttgcctgtg gagtatcagc ctgcatctgg ttgagcgccc tgatatggcg
     6841 gtgctgttat ttccgtttgg tctgcgtctg gggctaatgc tgcaatgccc gcgcggatac
     6901 tggccggtgc tgctgggcgc ggagtggctg ctgatttact ggctaacgca ggcggtcggt
     6961 ttaacccatt ttccgttatt gatgatcggt agtttactga cgttactgcc cgtagcgctg
     7021 atctcgcgct atcgccatca gcgtgactgg cgcaccttgc tgttacaggg ggcggcgtta
     7081 acggcggcgg cgttgttgca atcgctgccc tggctttggc acggcaaaga gtcgtggaat
     7141 gcgctgttgc tgactttaac tggcggcctg acgctggccc cgatatgtct ggtgttctgg
     7201 cactatctcg ccaataacac ctggctgccg ctcggcccgt cactggtttc tcagccaatc
     7261 aactggcgcg ggcgacatct ggtctggtac ttgctgctgt ttgttatcag tctctggctc
     7321 cagttgggat tgccggacga actgtcgcgc tttacgccat tctgtctggc gctgccgatt
     7381 atcgcgctgg cctggcacta tggttggcaa ggggcgctga ttgcgacgtt gatgaacgcc
     7441 atcgcgctga tcgccagtca aacctggcgc gatcatccgg tggatttatt gctctcgctg
     7501 ctggtgcaaa gtctgacagg gttgttgctt ggcgctggca tccagcggtt gcgtgaactt
     7561 aaccagtcgc tgcaaaagga actggcgcgc aatcagcatc tggctgaacg gttgctggaa
     7621 accgaagaga gcgtgcgccg tgatgtggcg cgtgagctgc atgatgatat cggtcagacc
     7681 atcactgcta ttcgtactca ggcgggcatt gttcagcggc tggcggcaga taacgccagc
     7741 gtgaagcaga gcgggcagct catcgaacaa ctatcgctgg gcgtttacga cgcggtgcgc
     7801 cgtttgttgg gtcggttacg tccgcgccag ttggatgatc tcaccctgga gcaggccatc
     7861 cgctcactga tgcgggaaat ggagctggaa gggcgcggta ttgtcagcca tctcgaatgg
     7921 cgaatcgatg aatcagcgtt aagcgaaaac cagcgcgtga cgctgtttcg tgtctgccag
     7981 gaagggctga acaacattgt gaaacatgct gatgccagcg cggtcaccct gcaaggctgg
     8041 cagcaggatg aacggttgat gctggttatt gaagacgatg gcagcggttt gccgccgggt
     8101 tccgggcaac aaggttttgg cctcaccgga atgcgcgagc gcgtaacggc gctgggtggc
     8161 acattacaca tttcctgtct gcacggcacg cgtgtcagcg tttctctacc tcaacgctat
     8221 gtctaaggtt tgatgatgtt gccgtttctg aaagcgcctg ccgatgcgcc attaatgact
     8281 gataaatatg aaattgatgc ccgctatcgc tactggcgtc ggcatattct gctgaccatc
     8341 tggctgggtt acgcgctgtt ttacttcacg cggaaaagtt ttaacgccgc cgtaccagaa
     8401 atccttgcta acggcgtgct cagccgtagc gatatcggcc tgttagcgac cctgttttac
     8461 attacctatg gcgtgtcgaa gtttgtctcc ggcattgtca gcgatcgctc aaatgcccgt
     8521 tattttatgg ggatagggct tatcgccacg ggcattatca acattctgtt tggcttctcg
     8581 acgtcgctat gggcgtttgc cgtactctgg gtgctgaacg cctttttcca gggctggggt
     8641 tcaccggtgt gtgcgcgtct gttaacggcc tggtattcac gtaccgagcg cggtggttgg
     8701 tgggcattat ggaacacggc gcataacgtc ggcggcgcac tcattcccat tgtgatggca
     8761 gcggctgcac tacattacgg ctggcgtgcc gggatgatga ttgctggttg tatggcgatc
     8821 gtcgtgggaa tttttctctg ctggcggctg cgcgatcgcc cgcaggcgtt aggtttaccg
     8881 gcggtcggtg aatggcgaca cgacgcgctg gagatagctc aacaacaaga aggggcaggg
     8941 ctgacgcgta aagagatcct caccaaatat gtgttgctga atccgtatat ctggctgctt
     9001 tcgttttgct atgtgctggt ctatgtggtc cgggcggcga tcaacgactg gggcaatttg
     9061 tatatgtccg agacgctggg cgtcgatctg gtcacggcga atacggcagt gacgatgttt
     9121 gaactgggtg gatttatcgg tgcgctggta gcgggttggg gctcggacaa attgtttaac
     9181 ggcaaccgag ggccgatgaa tttgattttc gccgccggaa ttttgctttc agtcggctcc
     9241 ctgtggctga tgccatttgc cagctacgtg atgcaggcaa cctgcttctt caccattggt
     9301 ttttttgtct ttggcccaca gatgttaatc ggtatggcgg cggcagagtg ttcccacaaa
     9361 gaggcggcag gggcggcgac ggggtttgtc ggcttgtttg cttatctggg ggcgtcgctt
     9421 gctggttggc cgctggcgaa agtactcgat acctggcact ggagcggatt ttttgtggtt
     9481 atctctatcg ccgccgggat ttccgcactg ctgttactgc cctttttgaa cgcccagaca
     9541 ccgcgcgaag cgtgatgcat ctcacctttt cacttcatat ccggcaaaac taagaaattt
     9601 tccaggtttt gcctggacgc tatctcaggc ctgatttgct gctgattttt acaatgcatg
     9661 cctcacgcag gtattcattt caggagtaac ccatgctggc tttcctaaac caggttcgca
     9721 agccgaccct ggaccttccg ctcgaagtgc ggcgcaaaat gtggttcaaa ccgttcatgc
     9781 aatcctacct ggtggtcttt atcggctacc tgacgatgta cctgattcgc aagaacttta
     9841 acatcgcgca gaacgatatg atttcgacct acgggttgag catgacgcag ctggggatga
     9901 tcggcctggg tttctccatc acttatggcg tgggtaaaac gttggtttca tactacgccg
     9961 acggcaaaaa caccaaacaa ttcctgccgt tcatgctgat cctgtctgcc atttgtatgc
    10021 tgggcttcag tgccagtatg ggcagcggct cggttagcct gttcctgatg attgccttct
    10081 acgccttaag cggctttttc cagagtaccg gcggttcgtg cagttactcc accatcacca
    10141 aatggacgcc gcgtcgtaaa cgcgggacat tcctcggttt ctggaatatt tctcacaacc
    10201 ttggcggtgc aggcgcagca ggtgtggcgc tgttcggggc aaattacctg ttcgatggcc
    10261 atgtcatcgg catgtttatc ttcccgtcga ttatcgcgct gattgtcggt tttatcggcc
    10321 tgcgttacgg cagcgactcc ccagaatctt atggcctcgg caaagctgaa gaactgttcg
    10381 gcgaggagat cagcgaagag gacaaagaga cagaatctac cgatatgacc aagtggcaga
    10441 tctttgttga gtatgtgctg aaaaacaaag tgatctggct gctgtgcttc gccaacattt
    10501 tcctctatgt ggtacgtatt ggtatcgacc agtggtcaac cgtatacgcg ttccaggaac
    10561 tgaaactctc taaagcggtg gcgattcagg gctttacgct gtttgaagct ggtgcgctgg
    10621 tcggtacgct gctgtggggc tggctctctg acctggcgaa cggtcgccgt ggcctggtgg
    10681 cctgcatcgc gctggcgctg attatcgcca cgctcggtgt gtatcaacat gccagcaacg
    10741 aatatatcta tctggcttct ctctttgcgt tgggtttcct ggtctttggc ccgcaattgt
    10801 tgattggtgt ggctgctgtt ggctttgtac ctaaaaaagc gattggcgct gccgatggta
    10861 ttaaaggcac ctttgcttac ctgattggtg acagctttgc caagttaggt ctgggaatga
    10921 ttgccgatgg gacgccggta ttcggcctta ccggctgggc aggcaccttc gccgcacttg
    10981 atatcgccgc gattggttgt atctgcctga tggcgatagt ggcggtgatg gaagaacgca
    11041 aaatccgccg cgagaaaaaa attcagcagt tgacagtggc ataaacgtaa ctggtgactt
    11101 ttgcccggca tgacgccggg ctt
//
LOCUS       DAJTSL010000133        11082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTSL010000133 DAJTSL010000000
VERSION     DAJTSL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.133"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..212)
                     /locus_tag="OO433_003155"
                     /pseudo
     CDS             complement(1..212)
                     /locus_tag="OO433_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            489..1364
                     /locus_tag="OO433_003156"
     CDS             489..1364
                     /locus_tag="OO433_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCR6051536.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLIDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSDQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKNMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1372..2148
                     /locus_tag="OO433_003157"
     CDS             1372..2148
                     /locus_tag="OO433_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR6051537.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLTFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2317..4578
                     /locus_tag="OO433_003158"
     CDS             2317..4578
                     /locus_tag="OO433_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6051538.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTAHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDESHPANSA
                     RGRTWIFGGDIRF"
     gene            4647..5822
                     /gene="senB"
                     /locus_tag="OO433_003159"
     CDS             4647..5822
                     /gene="senB"
                     /locus_tag="OO433_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCR6051539.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            6281..6631
                     /locus_tag="OO433_003160"
     CDS             6281..6631
                     /locus_tag="OO433_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6051540.1"
                     /translation="MPSGRHTARIRTGHDSGTVSPFVHSTLSPLVPVYPVFITVIADH
                     DKPQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEELSNLVLLHPNCHR
                     QLHSCEAGSLKKGL"
     gene            6774..6995
                     /locus_tag="OO433_003161"
                     /pseudo
     CDS             6774..6995
                     /locus_tag="OO433_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7016..7078)
                     /locus_tag="OO433_003162"
                     /pseudo
     CDS             complement(7016..7078)
                     /locus_tag="OO433_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            7529..8941
                     /gene="ospC1"
                     /locus_tag="OO433_003163"
     CDS             7529..8941
                     /gene="ospC1"
                     /locus_tag="OO433_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6051541.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQTLFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(9045..9193)
                     /locus_tag="OO433_003164"
                     /pseudo
     CDS             complement(9045..9193)
                     /locus_tag="OO433_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            9269..10966
                     /gene="ospD3"
                     /locus_tag="OO433_003165"
     CDS             9269..10966
                     /gene="ospD3"
                     /locus_tag="OO433_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6051542.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGKLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKI"
BASE COUNT         3411 a         2360 c         2353 g         2958 t
ORIGIN      
        1 gggatgtgct gtttcagcct cgcgaccagc tcacgctgtg tcagcgtttc cgtcgccgtt
       61 tctcccgttt tgtggtccag gtaacggaag ctcaggctcg cccctccgtt gtaatgtgcc
      121 agccgggagg ccgctatcgg cggcttcttc agataacgac ccaggtagcg cgccgtattc
      181 ctcccgccgg ccattttctt cgacatgtac atcaggcgcg gaactcatcg gggcgcggga
      241 atgaaatccc attcagactc cggatagatt caagcaagcc cacttatcgt caaaatcggt
      301 gttgcaaaaa cgggagtgac catagatccg ttttccaagc ggaccccata atatcggctg
      361 gtaaacatca ctcgctagtg cattcttttt gttggtaatg ataattattt acatttattc
      421 tttgatgttt tatcataatt aatgttttat caataaatat atggaaatat attgtttcag
      481 atgggaatat gagaaagttc atccttattt ctatgataac gttagctagt gttagtctgg
      541 gagcctgcag acagaatgtc accataaagc aggatgcgcc agggcagaaa gctttcctga
      601 ttgacgggca gatttacgat ttgcacagtg gcaaaattat ttctagcagc gagcttttgg
      661 cggatcttgc taccgctcga catcttatta ttggtgaaaa acacgataat gccgagcatc
      721 atcagattga gctttggctt attcaaaatc tgctcatcca gcgaccacag gggagtgtgt
      781 tgcttgagat gttgacgtca gaccaacagc cccgagttaa ccaagttaaa tgttggctga
      841 aagataatcc tgtggttcga gatagccgtg ttcaggaact acttaactgg caaaaagggt
      901 ggtcctggga gatgtatggc gacatcgtca tgcagctatt acgtgggcct tacccgctgc
      961 tcaatgccaa cattggccgg gagcagatgt tggcactgta caaaaaaaac gaatttccta
     1021 aaggaaaaaa atccactgca cctgtggtac aagaagctct gcgtgaaacc atcatagcta
     1081 tgcatgaagg caaccttgaa agccagcaac tgacgtcaat gctctctgtt cagcagcagc
     1141 gggatcgcta catggcccgc cagcttctga gtgccccggt accgtcgttg ttgattgcag
     1201 gtggttatca cgcgtcaaaa aacatgggcg taccgttaca catggaagat cttgccaccg
     1261 gcacccatcc agtggtgttg atgcttgcag aaaaaggaat gaacatcaca gtagatcatg
     1321 ctgactacgt gtggtttgta gcgccagata caacaaaaag gtaaaccatg gatgatgaat
     1381 attctccact tcccacagtc ggtaaaatgg agcagctggt ttatctgttc tctactgctt
     1441 catggactga ttttccttac gtttatctgg cgtttttccg aagttcagcc cgctatgtct
     1501 cctgcaccgg cgatcatgct gcaatgggca gaaattatag aggctccttc ttctccactt
     1561 tcgcttccgg tgggaatcgc tcaacaggag tccgcagtga cagaagaaaa gcaacaaact
     1621 gaggacagac aacaacgtcc tgtcactgaa gacagtgatg caaccattga aataactagg
     1681 aagaaaaagt catccgatgg agaaaagaaa aagacacgtc cacctcgtaa gataaaggct
     1741 caaaccagcg acagcaaccc cacagccgtt tccagtaatg ctgcacctca ggctctggtc
     1801 gaatcgtccc gtattgctgc gccttttaac agtgactcaa caaagcgaga taattctgag
     1861 gcatcttggg aaagcagagt aaaaggccac cttaaccgtt ataaacgtta ccctggcgat
     1921 gccagaaaac gtgcacgaac cggcacagcc gttgtgactt ttaccgtaaa cacggaaggc
     1981 actattgtca gtagtttctt ggaaatctcg tcaggaaccc tttctcttga ccgcgaagct
     2041 attgctgttt tagaacgcgc tcaaccactt cccaaaccac cgcctgaaat attggaggga
     2101 ggattattta aagtaaaaat gccgattacc tttaagttga aggagtaaag ggataattgg
     2161 ccgttcaagt tcatttaact gaaatttaat tttcattttt atgctgaaca ttttatcaaa
     2221 cttttccact gtcgaaaaaa tcaaaaagac agtagatcat aattaataaa caaatagtta
     2281 tacttatctt tattaatatt atatccaagg aaatttatga acgttataaa actggctatc
     2341 ggctcaggca tattattgct cagctgcggt gcttactcac aatccatcag tgaaaaaact
     2401 aattccgaca aaaaaggagc ggcagaattc agtccgctca gcgtttctgt cgggaagacg
     2461 accagtgagc aggaagctct cgagaaaaca ggcgcgacca gttcccggac aacggacaaa
     2521 aacctgcaat cacttgacgc aacagtgcgt agtatgcctg gtacttatac tcaaatagat
     2581 cctggtcagg gagcaatcag tgtgaatatt cgaggcatga gcggatttgg tcgtgtaaac
     2641 actatggtcg atggtattac ccagagtttt tacggaacct ctacctccgg aacaacggcg
     2701 catggttcaa ctaacaatat ggctggcgta cttatagatc ctaacttact ggtagcagtt
     2761 gatgttacac gcggtgacag cagtggctct gaagggatca acgcccttgc cggtagtgca
     2821 aatatgcgta ctattggcgt tgacgatgta atatttaacg gtaatacata tggccttcgt
     2881 tcacgtttct ctgtcggtag taatgggctg ggacgcagcg gaatgatcgc ccttggtgga
     2941 aaaagcgacg cttttacgga tacgggaagc attggcgtta tggctgctgt gagtggcagt
     3001 tctgtgtact ctaatttctc aaatggttct ggaattaaca gcaaagagtt tggttatgat
     3061 aaatatatga agcagaaccc caaatcccaa ctgtataaaa tggatatcag accagacgaa
     3121 tttaacagct tcgaactttc cgctcgaacc tatgaaaata aatttacacg tcgtgatata
     3181 accagtgacg actattacat taaatatcat tacacccctt tttctgaatt aattgacttt
     3241 aacgtaacgg ccagtaccag tcgcggtaat caaaagtatc gtgatggctc gctgtatact
     3301 ttctacaaaa cctcagcgca aaatcgttct gacgcgctgg atatcaacaa taccagccgg
     3361 ttcactgtcg cggacaatga actggagttt atgctgggca gcaaactgat gcgtacccgc
     3421 tatgaccgga ccattcactc agcggcgggc gacccgaaag cgaatcagga atcgatcgag
     3481 aacaatccgt tcgcaccctc cggccagcag gatatttcag cgctgtatac cgggctgaag
     3541 gttacgcgcg gcatctggga ggcagatttc aatctcaact acacacgtaa caggatcaca
     3601 gggtacaagc ccgcctgcga ttcacgcgtt atctgcgtgc cacagggtag ctacgatatt
     3661 gacgataaag agggtggctt caacccttca gttcagcttt ctgctcaggt aacaccatgg
     3721 cttcagccgt tcattggcta cagcaaatcc atgcgcgccc cgaacatcca ggagatgttc
     3781 ttctctaatt caggaggcgc atccatgaac ccattcctga agcctgaacg tgcagaaacc
     3841 tggcaggcgg gttttaacat tgataccaga gatttactgg tcgaacagga tgccctgcgc
     3901 tttaaggctc tggcgtaccg cagcaggatc cagaactaca tctacagcga gtcttatctg
     3961 gtttgttctg gaggtcgtaa atgcagtatg gctgaggtga ttggcaatga ctgggagggc
     4021 attagcgatg aatacagcga caatatgtac atctacgtta actcggcaag cgacgttatt
     4081 gcaaagggct tcgaactgga gatggattat gatgcaggtt ttgcttttgg ccgactctct
     4141 ttcagccagc agcaaacaga ccagccaacc tccatcgcca gcacctactt tggcgcaggg
     4201 gatatgaccg aactgcccag aaaatacatg acgctggata ctggtgttcg cttcttcgat
     4261 aacgcgttga ccctgggcac tatcataaaa tacacaggca aggctcgtcg cctgtcgcct
     4321 gattttgagc aggacgaaca taccggcgca ataatcaaac aggatttgcc gcagatccca
     4381 acgattatcg atctctatgg tacttacgag tacaaccgca acctgacact gaaactttcg
     4441 gtacaaaacc tgatgaacag agattattcg gaggcgctga ataagctcaa catgatgcca
     4501 ggtcttggtg acgagagcca cccagccaat tccgcgcgtg gcagaacatg gatatttggc
     4561 ggggacattc gtttctgatg acaatttccg ccaggttcag acaatacgtt ttctctctta
     4621 tgtcaatttt attgcaggaa cgaaaaatga atattttcac tttatccaaa gcaccgctat
     4681 acttgttaat ttcactattt ttacccacga tggccatggc tatcgatcca cctgaacgcg
     4741 aactttcgcg atttgccctg aaaacgaatt accttcagtc ccctgatgaa ggcgtctatg
     4801 aactggcgtt tgataatgcc agtaaaaagg tgtttgcagc agtcaccgat cgtgtaaatc
     4861 gtgaagccaa taaaggctat ctgtattcgt ttaattcaga ttcgctgaaa gtcgaaaata
     4921 aatacacgat gccataccgg gcattttcgc tggcgataaa tcaggataaa catcagctct
     4981 atatcggaca cacccagtca gcgtccctgc gtatcagtat gtttgacacc ccaaccggta
     5041 aactggtaag aaccagcgac aggttaagtt ttaaagcggc aaacgctgca gattcgcgtt
     5101 ttgagcattt gcgccatatg gtttacagcc aggattccga taccctgttt gtgagttata
     5161 gcaatatgct gaaaacggcc gagggcatga agcctctgca taagctgtta atgctcgacg
     5221 ggacgacgct tgccttaaaa ggcgaggtta aggatgctta caaaggtaca gcgtatggtc
     5281 tgacgatgga tgaaaaaaca cagaaaatct acgttggcgg aagagattac atcaacgaaa
     5341 ttgatgcgaa aaatcagacg ctgctgcgta ccatcccgtt gaaagatccg agaccacaaa
     5401 tcacaagtgt gcagaatctg gcggtggact ccgcttctga ccgtgccttt gtggtggtat
     5461 tcgaccatga cgatcgttcc ggtacaaaag atggcctcta tatttttgac ttacgcgacg
     5521 gtaaacagct tggctatgtg cacacaggag ccggagctaa cgcggtgaaa tacaatccga
     5581 aatataacga actgtatgtc accaacttca ctagcggcac catcagcgta gtggatgcca
     5641 ccaaatacag catcacccgt gaatttaaca tgccggtcta cccaaaccag atggtgttgt
     5701 cggacgatat ggataccctt tacattggca tcaaagaagg ctttaaccgc gattgggatc
     5761 ctgatgtgtt tgtggaagga gctaaagaac gtattctgag cattgatttg aaaaagtcgt
     5821 gatcggggga aaaaacatgg gataaaaact ccgttattcc actaaactgg ccggactagg
     5881 aaaagcaaac atctctgtcc tgccggtacg cctgttacgc cgctccatga ggctgaggaa
     5941 tctccagaaa acgagacagg cacagcattg tacgatcaac tgatttgtgc caataattca
     6001 atgaaatcac agctatgcct gttcacgtat aggaaattga aaaactaagc gataccactg
     6061 tccgcgtctt atgcctcgtg cggtaagctg tttttcacat cacgccggtg tttttttctt
     6121 tctgtggacg gttttaatac tgtaatcggc cgttttttat acttaccccg attcccgggc
     6181 gcacttatcc tgcatcaccc cgggatatac ctcagcagac tgatatcccg cgcgtttttc
     6241 ttcagcccct ccacattcag tcccgcgatg gcccggcggt ataccatccg gccgccacac
     6301 agcacgcatt cgaacgggtc acgactcagg aactgtttca ccatttgtgc atagcacact
     6361 ttcgccactg gttccggttt atcctgtttt tataacggta attgcagacc atgacaaacc
     6421 gcagccgtca ggctgcctac tcgagtcaca aggctctctg tgtccgatat gccggcagcg
     6481 cattacccat gaaactggct ggaacgtaca ccataaggtg aagaaagtta tggggggcgg
     6541 tgaagaatta tctaaccttg tcttgttaca tccaaattgc catcgacagt tacacagttg
     6601 tgaagccggc tctctcaaaa agggcttata aaggcttgag cggtgtaccg ggaaaccggt
     6661 atgcaccgtt cttaggggag ggcagtccag caatgaactg tccttacccg acaaacatcc
     6721 aaaacgaacg gaacgtgctg gaatccgcat ataacccgtt gtaaagtgag gaaatggaca
     6781 cctcacttgc tcatgagaac gcccgcctgc gggcactgtt gcagacgcaa caggacacta
     6841 tccaccagat ggctgaatac aaccgcctgc tctcacagcg gatggcggct tatgcttccg
     6901 aaatcaaccg actgaaggcg ctggttgcga aactgcagcg tatgcagttc ggtaaaagcc
     6961 cagaaaaact tcgcgcaaaa accgaacggc agattccatt ttcacgggcg atctacgcca
     7021 cacaggctct gggttgcagt gactgctcca ttaaggcgat tttgaactca taagggaagt
     7081 aacaaaaaaa ctgcaaaagt acaataatta tatatggcca aactaacctc tattaaaaaa
     7141 acatcagttt ttcacactta gttgagcttc atgaaaaaat catcgaggat ttcattgcag
     7201 aatacacatt ccactaactg gaagcactcc ataagaggaa tcagagaaag tatcgttttt
     7261 ttatagtaaa attcattgct gttcaataaa attgatataa ttattaaatt atggaaataa
     7321 tttcacaaaa aagattaact tgagttctta agcatcggat tttagatatt ttgaaatatc
     7381 tggcatcaca taacatcaaa agtatataat acccttatct aattacatgc agtatattgt
     7441 atatatactc aaaaattgta attcataccc tactatatct tgtggggaaa gatattaatt
     7501 aaaactgttt tcatataagg ttcattttat gaatatatca gaaacactga actcagcaaa
     7561 tacccaatgc aatatagatt ctatggataa cagattacat acattgtttc caaaagtgac
     7621 atcagtgcga aacgctgcac aacaaactat gccagatgaa aaaaatttaa aagatagtgc
     7681 aaatattatt aaagatttct ttaggaaaac tatagcagca caaagttata gtagaatgtt
     7741 ctctcaaggc tctaacttta aatctttaaa tatagcaatt gatgcaccat cagacgctaa
     7801 agcctcattt aaggctattg agcaccttga cagattatcg aagcattata tatctgaaat
     7861 aagggaaaaa cttcatcctc tttctgcaga ggaactcaat ttgctttcgc taattattaa
     7921 ttctgattta atcttcagac atcaaagtaa ttctgatttg tctgataaaa ttttaaacat
     7981 taagtcattc aataaaattc agtctgaagg aatatgcaca aaacgaaaca catacgctga
     8041 tgatataaaa aaaatagcta atcatgactt tgtgtttttt ggcgttgaaa tctctagcca
     8101 tcagaaaaaa caccccctga atacaaaaca tcacactgtt gattttggtg caaatgcgta
     8161 tatcattgat catgactctc catatggata tatgacatta accgatcact ttgataatgc
     8221 tattccacct gttttttacc atgagcacca atcattttta gataaatttt cagaggttaa
     8281 taaagaagtt agtcgatacg tacatggaag taaaggaatt atagatgtac caatattcaa
     8341 tactaaagat atgaagttag ggctcggatt atacctgatt gactttatta gaaaaagtga
     8401 agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt gcccctgtgg atctggatag
     8461 aatcataaac tttgtttttc agccagagta ccatatacct aggatggtaa gtacagaaaa
     8521 cttcaaaaaa gttaagatta gagaaatatc cttagaggag gctgttacag catctaatta
     8581 cgaagaaatt aacaagcagg tcactaacaa aaaaattgct ctccagactc tttttctttc
     8641 gattactaat caaaaagagg atgtcgcctt atatatatta tctaattttg agataactag
     8701 acaagatgtt atttccataa agcatgagtt gtatgatatt gagtatctac ttagcgctca
     8761 taattcaagc tgtaaagtac ttgagtattt tatcaataag ggattggttg atgtaaacac
     8821 aaagttcaaa aaaactaata gtggggattg tatgttggat aacgcaataa aatatgagaa
     8881 tgcagaaatg ataaaactat tattgaaata tggtgcaaca tctgacaata aatatattta
     8941 atcaaaattg aatatcgttt agcaaaaggc agtatttgca caaaatatcg atatattgtt
     9001 tattgtcagt atggctcaat gtgataatag ttggaaagtt tgatgggttt cgccccgttg
     9061 tagcggtagt cgagctggaa ggtcttcagg cactgcttac agcgatagag cagcccccct
     9121 gaactggaat ggccgttccg gataccccct gagtttcaga gtaacgggga caaaccacat
     9181 caatctttgc catcaatcat ccaaagggca aagagtacaa caacactaag tctgcgtcac
     9241 aacccatcaa tgaaaggaat atatacatat gccatcagta aatttaatcc catcaaggaa
     9301 aatatgtttg caaaatatga taaataaaga caacgtctct gttgagacaa tccagtctct
     9361 attgcactca aaacaattgc catatttttc tgacaagagg agttttttat taaatctaaa
     9421 ttgccaagtt accgatcact ctggaaaact tattgtctgt cgacacttag cttcctactg
     9481 gatagcacag tttaacaaaa gtagtggtca cgtggattat catcactttg cttttccgga
     9541 tgaaattaaa aattatgttt cagtgagtga agaagaaaag gctattaatg tgcctgctat
     9601 tatttatttt gttgaaaacg gttcatgggg agatattatt ttttatattt tcaatgaaat
     9661 gatttttcat tccgaaaaaa gcagagcact agaaataagt acatcaaatc acaatatggc
     9721 attaggcttg aagattaaag aaactaaaaa tgggggggat tttgtcattc agctttatga
     9781 tcccaaccat acagcaactc atttacgagc agagtttaac aaatttaact tagctaaaat
     9841 aaaaaaactg actgtagata attttcttga tgaaaaacat cagaaatgtt atggtcttat
     9901 atccgacggt atgtctatat ttgtggacag acatactcca acaagcatgt cctccataat
     9961 cagatggcct aataatttac ttcaccccaa agttatttat cacgcgatgc gtatgggatt
    10021 gactgagcta atccaaaaag taacaagagt cgtacaacta tctgaccttt cagacaatac
    10081 gttagaatta cttttggcag ccaaaaatga cgatggtttg tcaggattgc ttttagcttt
    10141 acaaaatggg cattcagata caatcttagc atacggagaa ctcctggaaa cttctggact
    10201 taaccttgat aaaacggtag aactactaac tgcggaagga atgggaggac gaatatcggg
    10261 tttatcccaa gcacttcaaa atgggcatgc agaaactatc aaaacatacg gaaggcttct
    10321 caagaagaga gcaataaata tcgaatacaa taagctgaaa aatttgctga ccgcttatta
    10381 ttatgatgaa gtacacagac agatacccgg actaatgttt gctcttcaaa atggacatgc
    10441 agatgctata cgcgcatacg gtgagctcat tcttagcccc cctctcctca actcagagga
    10501 tattgtaaat ttgctggcct caaggagata tgacaatgtt cccggacttc tgttagcatt
    10561 gaataatgga caggctgatg caatcttagc ttatggtgat atcttgaatg aggcaaaact
    10621 taacttggat aaaaaagcag agctgttaga agcgaaagat tctaatggtt tatctggatt
    10681 gtttgtagcc ttgcataatg gatgtgtaga aacaattatt gcttatggga aaatacttca
    10741 cactgcagac cttactccac atcaggcatc aaaattactg gcagcagaag gcccaaatgg
    10801 ggtatctgga ttaattatag cttttcaaaa taggaatttt gaggcaataa aaacttatat
    10861 gggaataata aaaaatgaaa atattacacc tgaagaaata gcagaacact tggacaaaaa
    10921 aaatggaagt gattttctag aaattatgaa gaatataaaa atctgaatat tatctcgtgg
    10981 tgatacactg aatttgaggt aaccggctca tttaaaccgt ctggtctgtt acctccggtt
    11041 ccacaaaaat aatgtccatc attttgaagt ggacactatc gt
//
LOCUS       DAJTSL010000134        11061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTSL010000134 DAJTSL010000000
VERSION     DAJTSL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.134"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..587
                     /locus_tag="OO433_003166"
     CDS             <1..587
                     /locus_tag="OO433_003166"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6051543.1"
                     /translation="GNDTIVSVASCTTNCLAPMAKALHDSFGIEVGTMTTIHAYTGTQ
                     SLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLEIPELSGKLKGHAQRVPVKTGSVT
                     ELVSILGKKATAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEIT
                     AVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(629..2069)
                     /gene="aldA"
                     /locus_tag="OO433_003167"
                     /pseudo
     CDS             complement(629..2069)
                     /gene="aldA"
                     /locus_tag="OO433_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(2224..3066)
                     /locus_tag="OO433_003168"
     CDS             complement(2224..3066)
                     /locus_tag="OO433_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6051544.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALFNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLRQKLPVCEPLV
                     CKRA"
     gene            complement(3338..7240)
                     /gene="hrpA"
                     /locus_tag="OO433_003169"
     CDS             complement(3338..7240)
                     /gene="hrpA"
                     /locus_tag="OO433_003169"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6051545.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            7441..8046
                     /gene="azoR"
                     /locus_tag="OO433_003170"
     CDS             7441..8046
                     /gene="azoR"
                     /locus_tag="OO433_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6051546.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(8097..9413)
                     /locus_tag="OO433_003171"
     CDS             complement(8097..9413)
                     /locus_tag="OO433_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR6051547.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWPFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWPVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(9403..11061)
                     /locus_tag="OO433_003172"
                     /pseudo
     CDS             complement(9403..11061)
                     /locus_tag="OO433_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
BASE COUNT         2747 a         3017 c         2636 g         2661 t
ORIGIN      
        1 atggcaacga caccattgtt tccgtggcgt catgcaccac taactgtctt gcgccgatgg
       61 ccaaagcctt gcatgacagt ttcgggatag aagtcggcac gatgacgacc attcatgcct
      121 atactggcac ccagtcactg gtggatggcc cacgtggtaa agatttacgt gcttcacgcg
      181 cagcggcaga aaatatcatt ccccacacta cgggggcggc aaaagccatt ggtctggaga
      241 tcccggaact gagcggcaaa ctgaaaggtc atgcgcaacg cgtgccggtg aaaacaggtt
      301 cggtcactga actggtatcg attctcggaa aaaaagcgac tgccgaagag gtgaataacg
      361 cacttaaaca agcaaccacc aataacgagt catttggtta taccgatgaa gaaatagtct
      421 cttccgatat cattggcagc catttcggtt cggtgtttga tgccacgcaa acggaaatta
      481 ccgccgtggg cgatttacaa ctggtgaaaa cggtcgcctg gtacgataac gaatatggct
      541 tcgtcacgca gcttattcgc accctcgaaa aattcgctaa actctgacgc gcacaggcgg
      601 aggaaaaaac ctccgcctct tttactcatt acgactgtaa ataaaccacc tgggtctgca
      661 gatattcatg caggccatgt ttaccatctg cgccgccaat accggattta cgccatccgg
      721 cgtggaagcc ttgcatagct tcgaactttt cacggttgat gtaagtttca ccaaacttca
      781 gccctttaat ggctttcatc gcgacgttca gattttgggt atagatttga tgaggtcagg
      841 ccgtaatcac tgtcattagc cattgagata gcctcttcca gcgtgtcaaa tgcgaccacc
      901 ggcagaaccg ggccaaaggt ttcctcatgc ataatcgaca tttcctggcg aacatccagc
      961 agcaatgtcg gcggataata atatcctttc ccctctaccg ctttgccacc caacgccact
     1021 ctcgcccctt cttctactgc gcgcgccact ttttgctcaa ccctttccaa cgccgcggcg
     1081 ttaatcaacg gccccatcgc aatgtcgttg cgttcagcgg ggttaccaaa ttgaaccgcc
     1141 tgcatcgctt cacccagccg attgacgaac tgatcataaa tgcctttctg tacataaaca
     1201 cgttctgcac agttacacac ttgcccacta ttaatgacgc gtgaatcaac gatggctttg
     1261 actgccagtt caagatcggc atcgtccatt acgatagctg gtgctttacc ccccaattcc
     1321 agacacactt tggtgatgtt tttcgccgca gtcgccatga tcttctcacc tgcagagacg
     1381 ctgcctgtca tactgaccat tgcgaccttt gggttacccg ccagttcttg cccaacggtt
     1441 tcaccacgcc ccagtacaag gttaaacacg ccgcgcggaa ggcctatttc atcgacgatt
     1501 ttggcgaatg caatcgcatt gtttggcgta aattcactag gtttaatgac gatggtatta
     1561 ccggtcaaaa gagcgggagc cattttgcgg gcaatgagga agaacgggaa gttccacggc
     1621 agaatgccgg tagtcacacc aagcgcacgt ttaaacaaaa gaatattttc tcctggacga
     1681 tcgctttgaa taatctcgcc ctcgtaacgc cgtgcccact ccgccatgta atcgatatag
     1741 tcggcagtaa aagccacttc gacttcagcc agctgctgga tcttgccccc ttcttcaaca
     1801 atcagcgcac tgatttcact ggcgcgttcg cggatcccgg cggagatttt gcgcaaccaa
     1861 ctggcgcgtt caatagcagg caacgcttcc cattctggtt gtgcacgttc tgctgcatcg
     1921 attgccttac gggcatcctc ggcctgacca tcgggtatgc gggaaatgac agcctctgta
     1981 gcagggttta ccacatcaat ccatgcgtct ccacgccagg taacaaactg tccatcgata
     2041 tacataggat gttgaacggg tactgacatg ggcgactcct gtgatttata tgtttgttct
     2101 tggtaaattc attgttaatt aacatcaatc atcatagaga cggcatcgtg aaaggacaat
     2161 tctgtgaagg gcttcataaa aacaagtgta aaaggaataa aaggcagaaa taaaagtcag
     2221 gagtcatgcc cgtttgcaaa caagtggttc acaaacgggc aatttttgac gtaacgagcg
     2281 actttccatc gcctcgatga gcaccgcatc gtgtttaagc gtttgccatg catggatgac
     2341 ttctgccgga aaatcaacgt gaacgataaa atctcgcccg cggggaccgt agccatcgct
     2401 atcatcgcgt aaacgcggca gctcgccagt gagtagtgag agataacgct caaccggcca
     2461 taatccttgt aactgattaa taaagattac actgtctgcg ttatttccta actgaggaac
     2521 gaatccggga taacttaacc agcgtggtgc atcgggattg tccccagtca tacggcggaa
     2581 cgtcgccatc gtgcgccgct gcatggtggg gtcctgaaca acgatagccg tatgaactcg
     2641 ttctacggcc tgattaaata gcgcgatgct aaagcgtgcg ttttcaccgc agtttgttga
     2701 ctggtcttca atccagattt tttcatgcgg aatgtgccag aactgatgag cgatatccgc
     2761 caggatggtc gcttctgctc tgccagtggt gcggatagtg ttgtagtgcg gatgctgtgc
     2821 gatggcgcta tacaaaaaag ttgtcgagtg accgatacca ccactaatca gtaaaggaat
     2881 ttgctgatcg cgagcaatct tacatgccgc gtcgatagtc ggcataaccg cattgcctgc
     2941 aaggatcacg caatcggcct gatacggcac ctcaccggag aaatcatcct gcgccagcca
     3001 ctgtccgata acatttatgg catctatagt tgccggcgaa agcgtcggaa acggggtaat
     3061 gttcatggtt cctccttatc gttcccttaa gaatactgcg tgagataata gtgagaagct
     3121 gagcactctg aaaggaggaa gtcgtatgtc cttaaccgcc actccgcaag cctgacatat
     3181 cttttaagga aatgctgtag ataaacatta ttcatcttgc agcagagaat tgtatttttc
     3241 tgaattcctg aatttttacc cttaccgttt tagaattaat aaaaaacccg gcgctaaatg
     3301 cttaccgggt tttagattta ttaggcaaat agcagggtta accgctaatc tgctccatcg
     3361 cctgcaaaat acgcttatct gaaatcggat aaggcgtacc aagttgttga gcgaagtaac
     3421 taacgcgcag ctcttctatc atccaacgga tctctttcac gtcttcatcc tcacgacgtg
     3481 caggcggcag tttgttgatc cattgctgcc acgcctgctg gacgttttcg actttcagca
     3541 tctgtgcacg atcgcgatgt ggatcaaccg ccagtttttc cagtcgtttt tcaatcgcct
     3601 gcaaatatcg cagcgtgtcg cccagccgtt tgaagccgtt accagtgaca aaaccgcgat
     3661 ataccaaccc gcccatctgc gctttaatgt cagaaagccc cagtgccatg gtcatatcca
     3721 cccgcccttt cagacgtttg ttgatattga acactgccgt aagaatttgc tcgacctgct
     3781 tcgcaatatc taccaccgtg tcgttcagtt cggcacgcac tttctcatgc agcgcagcaa
     3841 agccttcttc cgtccagacc gggccaccat tggcgtcgat cagttgatcc acaccgcagg
     3901 agatacagtc gtcgatcagc tccagcactt tgccatacgg gttgaagtac agtcccagct
     3961 tggctttgtt cggtaacttt tcatgtaaat acttgatcgg cgagggaata tttagcagca
     4021 gtagacggcg aagaccattc cacattgcct gtttttgctc cagcgggtta tcaaacagtt
     4081 tgatcgccac gctgtcgcgt tcatccacca gcgccggcca cgccttcact ttatagttgc
     4141 cacgcttctg ttcgtaactt tccggcagct gaccaaaact ccagatatgt aagccgctct
     4201 gctcgatacc gtcatccgcc accgcggaca gcgtttcctg cactttgcct ttcagcgcat
     4261 ctttcagatc ttgtagcgag cgcccttctt ttagcttctt gtttttgtca tccaccacgc
     4321 ggaaggtgat tttcaggtga tcgggcacct gatcccagtg ccagtcttcg cggtcaacgg
     4381 taacgccggt catccgccgt aactcgcgct caaggctgtc gagcaacggt aactccagcg
     4441 gtgtgacgcg gcctaaaaac gcttcggcgt agtttggtgc aggcacaaaa ttacggcgta
     4501 ccggtttcgg caacgattta atcagagcaa tcaccaattc gcggcgcaga ccggggatct
     4561 gccattcaaa cccgctttcc tcaacctggt taagtaacgg cagcggaata tgtacggtca
     4621 caccgtccgc gtctgcgcct ggctcaaact gatagctcaa acgcagcttg agattgccct
     4681 gatgccagaa gttcgggtaa tccagcttgc tgattttttc cgccccttct ttgatcaaca
     4741 tgctcttttc aaagttgagc aaatcaggcg tttcgcggct gacttttttc caccagctat
     4801 cgaagtgacg agcggagatt acatcgtggc tgatgcgctg gtcgtagaac tcaaacagcg
     4861 tttcgtcatc aaccagaata tcgcggcgac gtgatttgtg ttccagctct tccacttcgg
     4921 cccgcagttt caggttttca cggaagaatg cgtgacgcgt ctgccagtca ccttccacca
     4981 gcgcatggcg aataaagagt tcacgacata acgccggatc gatctggctg tagttgacct
     5041 tgcgcgcggc aacaatcggc aaaccataaa cagtgacttt ttccgttgcc atcaccgcgc
     5101 cctgcgcccg ttcccagtgc ggttcgctgt aggtacgttt aatcaaatgc tgagcgacag
     5161 gctccaccca ttccgggtcg atacgcgcag caatgcgccc ccacaggcgg ctggtttcta
     5221 ccagttccgc caccattacc catttcggcg gttttttgaa taaaccagaa ccggggaaga
     5281 tggagaaacg cgcgttacgt gcgccggtat attcttgttt atcggcatct ttcatgccga
     5341 tatgggaaag taaacccgtc agcaacgcaa tgtgaatttc gcgatactcc gccggttcgc
     5401 tgttaaccgg aatgccaagt tctttcacca cctgacgcaa ctgggtgtag atatcctgcc
     5461 attcgcgcac gcgcagatag ttgagataat cggtacgaca caggcgacgg aaggcgtttg
     5521 aagaaagcgc cttttgctgc tcgccaagat aattccacag atttacaaac gcgaggaagt
     5581 cggattcttt gtcgtggaag cgacgatgtt tttcgtccga tgcctgctgt ttgtccatcg
     5641 gccgctcacg cgggtcctga atggagagcg cggacgtgat aatcatcgcc tcacgcacgc
     5701 agccatgttt ctgcgcttcc agcaccatac gcgccagacg aggatcgacc ggcagttgcg
     5761 agagctggcg accgagcggc gtcagtttat aggcgctggc ctgttcatcc gtggtgatcg
     5821 cgcccagttc ttcgagcaga cgcacgccat cctggatatt gcgtttatcc ggtgcttcga
     5881 caaacgggaa cgcagcgata tcgcccagcc ccagcgcggt catctgcaaa ataaccgagg
     5941 ccaggttggt acgcagaatc tccggatcgg taaactccgg gcgcgagagg aaatcgtctt
     6001 cggaatagag acgaatacag atcccttcgg acacacgacc acaacggcct ttacgctgat
     6061 tggcagacgc ctgtgaaatg ggttcaatcg gcaaacgctg cactttggtg cgatagctgt
     6121 agcggctgat acgcgccgta ccggggtcga taacgtattt aatccccggt acagtcagcg
     6181 aggtttccgc gacgttggtc gccagcacaa tgcgtcgccc gctgtgcgac tggaacacgc
     6241 ggttctgttc gctattcgaa agccgcgcat aaagcggcaa gatttcggta tggcgtaagt
     6301 tcagcttgtt cagcgcatcg gcggtatcgc gaatttcccg ctcgccgctc ataaagatca
     6361 gaatgtcgcc agggctttcc tggctcagtt cgtctacggc gtcaaaaatc gcctgcaact
     6421 gatcgcgctc ggtgtcatcg gcttcttcaa caatcgggcg atagcgcact tccaccggat
     6481 aggtccgacc ggagacttcg ataatcggcg cattattaaa gtggcgagaa aagcgttccg
     6541 ggtcgatagt cgcggaagtg ataatgattt ttaggtcagg acgccgcggc agcaactctt
     6601 tcaaatagcc gagcagaaaa tcgatattca ggctgcgttc gtgtgcttcg tcaataatga
     6661 tggtgtcgta ctgcatcagc aggcggtctt gctggatctc cgccagcagg ataccgtcgg
     6721 tcatcagctt gaccatcgtg ttatcactta cgtgatcgct gaaacgcact ttgtaaccga
     6781 tgcaaccgcc tggctccgtt ttcagctctt ccgcaatacg gttcgccacc gttcttgccg
     6841 ccagacgacg cggctgggta tggccgatca gtcctttaat cccgcgcccc agctccatac
     6901 agattttcgg tagctgggtc gttttaccgg aaccggtttc cccggcgacg atcaccacct
     6961 ggtgatcacg aatcgcttcg agaatatcct gttttttctg actaactggc aaattgtccg
     7021 gataagtgat ttccggtcgt gccgcttcac gcagcaggac tttccctgcc gcctggtcaa
     7081 tctctttcgc catctcctgg aaaatggcct gttgtgcatc aggattttta accttcttaa
     7141 caccatgcag acggcgagaa aaacgcagtc tgtcgcgcag catcaacgaa tccagccgct
     7201 gctgcaaagc agtaaaggtc aatttttgtt gttctgtcat aacgttagtg ggcagtagtc
     7261 tgcctgatta attttctttt aaatgataga tatagagtac cacatcgcgg ctttctatgc
     7321 gttgttcaat aaattcgaac ataggcttcg atttattgcg ctatctctgt ggcaggattg
     7381 tgaataaagt gtcagcaagc aacggggcat cgccaattca aacatctata aggaaacacc
     7441 atgagcaagg tattagttct taaatccagc atcctggcag ggtactctca gtctaatcag
     7501 ttgtccgatt attttgttga acaatggcgc gaaaagcact ccgctgatga aatcaccgtt
     7561 cgcgacctgg ctgcaaatcc gattccggta ctggatggcg aactggttgg cgctctgcgt
     7621 ccgagcgatg cgccgctgac tccgcgtcag caggaagctc tggcgctttc cgatgagctg
     7681 attgccgagc tgaaagccca cgacgttatc gttattgcgg caccgatgta taacttcaac
     7741 atctcaactc agttgaaaaa ctattttgac ctggttgcac gcgcaggcgt tactttccgc
     7801 tataccgaga acggtccgga aggtctggta acgggtaaaa aagccatcgt tattaccagc
     7861 cgcggtggga tccacaaaga tggaccaacg gacctggtga cgccgtatct gtccacgttc
     7921 ctcggcttta tcggcattac cgatgtgaaa tttgtcttcg ccgaagggat cgcatacggt
     7981 ccggaaatgg cagcgaaagc gcagtctgac gcgaaagcgg ccatcgacag cattgttgct
     8041 gcataaccgg acacgatctt gatgtggact gtagttcagt ccacatctca atccacttac
     8101 ctgttttccc ataatttcag catcgctttg tgatcttcct taagcacaat atgcgaacgt
     8161 ctggctcgaa tataactaat cgcttcatca acggttttac agtgtccgta acataacaac
     8221 catgccgcca ctaccagcgc actgcgcgat aatcccaacg cgcaatggac cagaacgctg
     8281 ccttgctctt cgcgtaatgt ttccagcatc gccacggcct gtcggagctc cccctcttcc
     8341 ggaaccacca gatccagcat cggtacacag aaatagagtc gatcttttgt cgctcgtccc
     8401 cgagggaatt caaaggtgac gtccagaacc gcattctgtg ccggaatatg tcgtggaaac
     8461 gcccctaaat aaacaccagc agtaattttg ctcaccggct ccaggcgacg acaaaaccaa
     8521 cgcatagaca gccacatccc gatgcgccag ggcaatgtca gccagtaaac ggcgggcggg
     8581 agtttcccct gactatcttt ccctgttgtt atcgcgccaa gcccaccgta cccacggcca
     8641 ataatgagta gcgataatac tggccaacat aaccagactg gccaccataa ctgaatcatc
     8701 accattagct ccatcaacac aatgcacgag cccgcgccta cgacatatgg cagtgctatt
     8761 ttgattcgac gttgatcagg tttctgataa ttccaacgac gatcgacggg caccatccag
     8821 tcaatcaaca tacctaccgc cagccctgtg atgacatcaa taaaatgatg ctgccaggtc
     8881 gtcagagtag aaatagcgat gagtaaaaac catccgccac agactttacg ccacctctca
     8941 gccagatgct gacgaaagtg acgccaaagt agccagcaga gaataatatg cagcgaagga
     9001 gactggttat aaggcagatc aaacagttca agttgcgaaa atggccatcc cgtcacccca
     9061 ctcacttcag gacggataaa actaaacttc agcggataga gcaaaaaacc gcagcaggcc
     9121 attaccgttg ccagaataag ccggtggaca agtcggcgct gttcgaatgt cgtgctacaa
     9181 acgaacagcg aaaatccata taaaagatcc agactccagt aaggaacaat agtccaggga
     9241 agaaaaggga tcgccgtttc ccaaccgaat acctgactgg ggatatcatg gctgttaagg
     9301 tcctgaaccg cggtgaactg attaagagat ccataggtga agaaaaaaaa cggggccagc
     9361 aacaataacc agccagcgcc ttgtagcaat acgttccgac gttcagttat cacgacgcac
     9421 cgccatcgaa accgtaaaaa taccccactc atcaatccgt tgtgtgcatt tatcaaatcc
     9481 ggcatcacgc acgagtgaat ccatctcccc ttgcgaacgc acgcgcatta cccacggttt
     9541 accatcttta tgactggtta acaccccggc aatcatctcc agttgagggt gccacggctg
     9601 cccggtgtag atgagaatgc cgaccggttc gatggcattg gcaagacctg cgagtgagtt
     9661 ttttacctgc tcattttcgg gaaaaagctc atacagtcca gagacaattg ccagcgtagg
     9721 ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt caaaacgcac
     9781 ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat ttaactcgct
     9841 gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat ccagtacata
     9901 gcgcccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg cgtggagatc
     9961 ggcaacggct tgtttaatca gtatttgtaa atgggttttg cgctggcgaa taccgcgcca
    10021 gccaacactg ttcaggtaga ttttgtcgat taagcgcccg aatgcgttac taccttgcgg
    10081 ttgattttga tagacataat ccagcgaact gcctgaatca aagccggtgc tcattccgag
    10141 atgcaggccc gcagagtgcg tcccgaacaa tttcatcgcc tttcgcataa agcgatacgc
    10201 taaatcaacc ggcgataata gcacgggtcc accagaaagt agccgccatt gatccgctga
    10261 tggtcctgtg cagtcttcat gctgataatc aaatttttgc gatttgttag catataaacg
    10321 actaataaag ctttgcattt tttcaaatgc cagcgccctg ttctcttcac ccaacgtgtc
    10381 gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc tctgataaaa
    10441 atcaatctgc ggttggcgat gcaccacata gtcatcgcct gatatcagaa gttgcgtggg
    10501 gagcgtaatc gccgccgcat ctctaataat acgttcagac gttttataga gatcgagcaa
    10561 gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg ccacccgttg
    10621 ccgatcgtgg gtcaaatagc gtcctttcac ataggaatta ataaaaaaca gaccacgcag
    10681 acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa ccttaaaggc
    10741 cggagaagcc agcaccagcc cgcgaattgc aggcgcataa tcatgaacct atgtggcaac
    10801 cagcactgcg ccgacgcttt gcgcgatcac aaccacctct tccagtccaa cctagctgtc
    10861 gctggcagca aaacggacaa attcatcgac atcctgcact gaacgcgcaa gagatgggct
    10921 ataaccacgc ggccccgaag tttttccatg cccccgagca tcccatgcat aaaaagcagt
    10981 atctggcatc gccagttcat caacgatatg ttgtagacga ccagaatgtt catggccacg
    11041 atgaaataag acaatgacct t
//
LOCUS       DAJTSL010000135        11043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTSL010000135 DAJTSL010000000
VERSION     DAJTSL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.135"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(169..375)
                     /locus_tag="OO433_003173"
     CDS             complement(169..375)
                     /locus_tag="OO433_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6051548.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
     gene            687..776
                     /gene="kdpF"
                     /locus_tag="OO433_003174"
     CDS             687..776
                     /gene="kdpF"
                     /locus_tag="OO433_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6051549.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            776..2449
                     /gene="kdpA"
                     /locus_tag="OO433_003175"
     CDS             776..2449
                     /gene="kdpA"
                     /locus_tag="OO433_003175"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6051550.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALSADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2472..4520
                     /gene="kdpB"
                     /locus_tag="OO433_003176"
     CDS             2472..4520
                     /gene="kdpB"
                     /locus_tag="OO433_003176"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR6051551.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADIAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSITNDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4529..5072
                     /gene="kdpC"
                     /locus_tag="OO433_003177"
                     /pseudo
     CDS             4529..5072
                     /gene="kdpC"
                     /locus_tag="OO433_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
     gene            5065..7749
                     /gene="kdpD"
                     /locus_tag="OO433_003178"
     CDS             5065..7749
                     /gene="kdpD"
                     /locus_tag="OO433_003178"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR6051552.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTDAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNALGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATIEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDTIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7746..8423
                     /gene="kdpE"
                     /locus_tag="OO433_003179"
     CDS             7746..8423
                     /gene="kdpE"
                     /locus_tag="OO433_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR6051553.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFHLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8639..8896)
                     /locus_tag="OO433_003180"
     CDS             complement(8639..8896)
                     /locus_tag="OO433_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051554.1"
                     /translation="MQKPLPVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            9019..>11043
                     /gene="speF"
                     /locus_tag="OO433_003181"
     CDS             9019..>11043
                     /gene="speF"
                     /locus_tag="OO433_003181"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR6051555.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPCISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRV
                     SMNPQEANYAYSRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGG"
BASE COUNT         2470 a         2812 c         3023 g         2738 t
ORIGIN      
        1 aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatattt ttcctgaata
       61 tatttcagct gggtaatacc atatctaaat atttacctga acatcttcct aataaaaaaa
      121 ccgccagcat ctccactagc ggttttcaat gtattcaaaa ctcggcaatc agtaaaaatc
      181 accggttgcc ttttcggcct gttccatcca aaccggttta tcgctggttt tcgaccagac
      241 gcggtgcagg tagctgtaaa agcgggcgcg gtctttccag aaaagcatga aaggcagcgc
      301 cagaacgccc gctgcaaccg catatgtacg gcgtaaaaag ataagccatg caggatattc
      361 tctgtagagt tccatacttt tctccccctt ttgtatctac ccggtgaata gcaccggaaa
      421 aatgaatttg tttatctgat gaaaatagta ccgccttttg tgtaatttta ctactcatcc
      481 gaccacttat ttttgcttat tgatggttta tttacattca ctttgtaatt aagttgcaca
      541 aaagttaaat taatgctaaa cattagttaa accatggctt ttgccatttt tatacttttt
      601 tacaccccgc ccgcagattt ttgcgaaatc tttgcagcca gaattctact cttccggtat
      661 cacttttagg ccactggagg tgcactgtga gtgcaggcgt gatagccggc gtgttgctgg
      721 tgtttttatt actgggttat ttggtttatg ccctgatcaa tgcggaggcg ttctgatggc
      781 tgcgcaaggg ttcttactga ttgccacgtt tttactggta ttaatggtgc tggcgcgtcc
      841 tttaggcagc gggctggcgc ggctgattaa tgacattcct cttccaggta cagcgggcgt
      901 tgaacgtata ctctttcgtt tacctggcgt ctctgaccat gagatgaact ggaagcaata
      961 tctttgtgcc attctcggcc tgaacatgct ggggctggcg gtgctgtttt ttatgttgct
     1021 cggtcagcac tatctgccgc ttaatccaca gcagttgccg gggctgtcgt gggatctggc
     1081 gctgaatacc gccgtcagct ttgtcacgaa taccaactgg caatcttata gcggtgaaac
     1141 cacgttgagc tatttcagcc agatggcagg cttaacggta caaaactttc tttctgccgc
     1201 cagcgggatt gcggtgattt ttgccttcat ccgtgcgttt actcgccaga gcatgagcac
     1261 actcgggaat gcctgggtcg atctgctacg catcacgtta tgggtgctag tccctgtggc
     1321 gttgttgatc gcactgtttt ttattcaaca aggtgcgctg caaaactttc tgccctatca
     1381 ggcagtgaat accgttgaag gagcgcaaca gctgttaccc atgggaccag tagcttctca
     1441 ggaagcgatc aagatgctcg gtactaacgg cggtggcttc tttaatgcca actcgtcgca
     1501 tccgtttgaa aacccaaccg cactgaccaa cttcgtgcag atgctggcga tcttcttgat
     1561 cccaacagct ctgtgctttg cctttggtga agtgacgggc gatcgccgcc aggggcgcat
     1621 gttgctgtgg gcgatgtcag tgatttttgt catctgcgta ggcgtggtta tgtgggcaga
     1681 agttcagggt aatcctcatc tgctggcgct cagcgcagac agcagcatca atatggaagg
     1741 taaagagagc cgtttcggcg tgctggtcag tagcctgttt gcggtcgtga ctacggcggc
     1801 gtcctgtggc gcggtgattg cgatgcatga ttcgtttacc gctctcggcg gcatggtgcc
     1861 gatgtggcta atgcaaattg gtgaagtggt gttcggcggt gtcggttctg gtctttacgg
     1921 catgatgctg tttgtcctgc tggcggtgtt tattgccggg ctgatgattg gtcgtacacc
     1981 ggaatatctg ggtaaaaaaa tcgacgtacg cgagatgaaa ctgactgcac tggcgattct
     2041 ggttactcca acactggtac tgatgggcgc ggcgttggcg atgatgaccg acgccggacg
     2101 tagcgccatg cttaaccctg gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcgtc
     2161 cgccgctaac aacaacggca gcgcctttgc cggattaagc gccaactctc cgttctggaa
     2221 ctgtttactg gcgttctgca tgtttgtcgg tcgcttcggg gtgattatcc cggtgatggc
     2281 aattgccggt tcgctggtga gtaaaaagag ccaacccgcc agctccggca cgctgccaac
     2341 gcacggcccg ctgtttgttg gcctgttaat cggcaccgtg ttgctggttg gcgcactgac
     2401 ctttatccct gccctggcgc ttggtccggt ggcggaatat ctctcctgat gatattgagt
     2461 gagcactgaa tatgagtcgt aaacaactgg cgctattcga accaacactt gtcgttcagg
     2521 cgctgaaaga agcggtgaaa aaattaaacc cgcaggcgca atggcgcaat ccggtgatgt
     2581 ttatcgtctg gatcggcagt ctgctgacca cctgtattag catcgcgatg gcaagcggtg
     2641 cgatgcctgg caatgcgctg tttagcgcgg caattagcgg ctggctgtgg gtcaccgtac
     2701 tgttcgctaa tttcgccgag gcgctggcag aaggccgcag taaagcgcag gccaacagtc
     2761 ttaaaggggt gaaaaaaact gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg
     2821 cggcggacaa agttcctgcc gatcaactgc gtaaaggtga catcgtgctg gtagaagctg
     2881 gcgatattat cccctgcgat ggtgaagtca ttgaaggcgg cgcatcggtc gatgaaagcg
     2941 ccatcaccgg ggaatcggca ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca
     3001 ccggcggcac gcgtattctt tctgactggc tggtgattga gtgtagcgtt aaccccggtg
     3061 agacatttct ggatcggatg atcgcgatgg tggaaggcgc acagcgacgc aaaacaccga
     3121 acgagattgc cctgactatc ctgctgattg ctctgaccat cgtcttttta ctggcaaccg
     3181 ccacgctgtg gccgttttcc gcgtggggcg gtaatgccgt gagtgtaacg gtactggtgg
     3241 cgctgctggt ctgtctgatc ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg
     3301 ccgggatgag tcggatgctg ggcgcgaatg tgattgccac cagcggacgt gcagttgaag
     3361 cggcaggtga cgttgacgtt ctgctactgg ataaaaccgg caccatcaca ctcggtaacc
     3421 gtcaggcgtc ggagtttatc cctgcgcagg gcgtggatga aaaaacgctg gctgacgccg
     3481 cacaactggc ttcgctggct gatgaaacgc cggaaggccg cagtattgtg atcctcgcca
     3541 agcaacgttt taacctgcgc gagcgcgatg tgcagtcgct ccatgccacc tttgtaccgt
     3601 ttactgcgca aagccggatg agcgggatca acatcgacaa ccgcatgatc cgtaaaggtt
     3661 ctgtcgatgc cattcgccgc catgttgagg ctaacggcgg tcacttccct gccgatgttg
     3721 atcaaaaagt cgatcaggtt gcgcgtcagg gggccacgcc gctggtggtg gtggaaggtt
     3781 cccgtgtgct gggcgttatt gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct
     3841 tcgcccagct gcgcaaaatg ggcattaaaa cggtgatgat taccggcgat aaccgtctga
     3901 ctgccgccgc cattgctgcg gaagcgggcg tcgatgattt tctcgccgaa gcgacaccgg
     3961 aggccaagct ggcattgatt cgtcagtatc aggcggaagg ccgtttggtg gcgatgaccg
     4021 gcgacggcac caacgatgct ccggcgctgg cgcaagcaga tatcgcggtg gcgatgaact
     4081 ccggcaccca ggcggcgaaa gaggcgggca atatggtcga tctcgactct aacccgacca
     4141 agttgatcga ggtggtgcat attggcaaac agatgctgat gacccgtggc tcgctgacca
     4201 ccttcagcat taccaacgat gtggcgaaat acttcgccat tattccggcg gcgttcgcgg
     4261 caacgtatcc gcagttaaat gcgctgaaca tcatgcgcct gcattcgccc gactccgcca
     4321 ttctcagtgc agtcattttc aacgccttga ttatcgtctt tttgattcct ctggcgttaa
     4381 aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt gcgccgtaac ttatggattt
     4441 acggtctggg tgggctgctg gtgccgttta ttggtatcaa agtcattgat ttactgctga
     4501 ccgtttgcgg tctggtgtga ggtttaccat gcgtggctta cgtccggcat tatcgacatt
     4561 tatctttctg ttattgatta ctggcggcgt ttacccgctg ctgaccaccg tgctggggca
     4621 atggtggttt ccctggcagg ccaacggttc gttgattcgt gaaggtgata cggtgcgggg
     4681 ttcggcatta atcgggcaga attttaccgg caacggcaga aatgccctat aatccacagg
     4741 cttctggcgg gagcaatctg gcggtcagta accctgagct ggataaacaa atagccgcac
     4801 gcgttgctgc attacgggcc gctaacccgg atgccagtac gaacgttccg gttgaactgg
     4861 tgacggcatc ggcaagcggg ctggacaata atatcacccc gcaagcggcg gcctggcaga
     4921 tcccacgcat cgcgaaagcg cgtaatctca gcgttgaaca gctcacgcag ctgatcgcaa
     4981 aatacaacca acaaccgctg gtgaaatata tcggccagcc ggtcgtcaac attgtcgaac
     5041 tcaatctggc gctggataag cttgatgaat aacgaaccct tacgtcccga ccccgatcgt
     5101 ctgctggaac aaactgccgc gccgcatcgg gggaagctga aagttttctt cggtgcctgt
     5161 gcaggcgtcg ggaagacctg ggcgatgctg gcagaagccc agcgactgcg ggcgcaaggg
     5221 ctggatattg tggttggcgt ggtagaaacc cacgggcgaa aagataccga cgcaatgctg
     5281 gaagggctgg ctgttctgcc gccaaaacgc ctggcgtacc gtgggcggca catctgcgag
     5341 tttgacctcg atgccgccct cgcccgccgt ccggcgctga tcttaatgga cgaactggcg
     5401 cacagtaatg cgctaggttc tcgtcaccct aaacgctggc aggacatcga agagctgctg
     5461 gaagcaggca ttgatgtttt cactaccgtc aacgttcagc atctggaaag tctgaatgat
     5521 gtggtcagcg gcgtcaccgg aattcaggta cgggaaaccg tgcccgatcc ttttttcgat
     5581 gccgccgacg acgtggttct ggtggacttg cccccggacg atctgcgcca gcggctgaaa
     5641 gaaggcaaag tctatattgc cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa
     5701 ggcaatctga tcgccctgcg cgaactggca ctgcgccgta ctgccgatcg cgttgatgag
     5761 caaatgcgcg cctggcgggg gcatcctggc gaagagaaag tctggcacac gcgcgacgcg
     5821 atccttttat gcatcggcca taacaccggt agtgagaagc tggtccgcgc ggcggcgcgg
     5881 ctggcgtcac ggctgggtag cgtctggcac gcggtgtatg ttgaaacccc tgccctgcac
     5941 cgcttaccgg aaaaaaaacg tcgggcaatt ctcagcgcct tacgtctggc gcaggagctg
     6001 ggcgcggaga cggcaacact ttctgatcca gcggaagaaa aagcagtagt gcgttatgcc
     6061 cgtgaacata atctcggcaa gattattctc ggtcgcccgg cctcgcgccg ctggtggcgt
     6121 cgggaaacgt ttgctgaccg actggcgcgc atcgcccccg atctcgatca ggtgctggtc
     6181 gcgcttgatg aaccacccgc ccgcacgatt aacaacgcgc cggatagccg ctcttttaaa
     6241 gacaagtggc gggtacagat tcagggatgc gtggttgccg ccgcgttatg cgccgttatc
     6301 actttaattg ccatgcagtg gctgatggcg tttgatgccg ccaacctggt gatgctgtat
     6361 ctgcttggcg tggtggtggt ggcgctattt tatggacgct ggccttcggt ggttgctacc
     6421 gtcattaatg tggtgagttt cgatctcttt tttatcgccc cacgcggcac gctcgccgtc
     6481 tctgatgtgc aatatctgct gaccttcgca gtgatgttaa ccgtcgggct ggtgatcggg
     6541 aaccttactg ctggcgtgcg ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca
     6601 cggcacttat atgaaatgtc gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc
     6661 gccaccatcg aacaatttat tgcctccacg tttcatgccc gcagtcaggt gttgttgccc
     6721 gatgacaacg gtaaattgca gccgttaaca catccgcaag gaatgacgcc atgggacgat
     6781 accatcgcgc agtggagttt tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta
     6841 cccggtgtac cgtaccagat tttgccgcta aaaagcggcg agaaaaccta cgggctggtg
     6901 gtggtggagc cggggaatct gcgccagttg atgatcccgg aacagcagcg cctgctggag
     6961 acgtttacgc tgttagtcgc caatgccctt gagcggctga cgctaaccgc cagcgaagaa
     7021 caggcgcgga tggcaagcga acgtgaacag atccgcaacg ccctactggc agcgctttcg
     7081 catgatttac gcacgccgct cacggtgctg ttcggtcagg cagaaatctt aacgctcgat
     7141 ctggcaagcg aaggatcgcc ccacgcccgc caggccagcg agattcgtca gcatgtgctg
     7201 aacactaccc gactggtgaa taatctactg gatatggcgc gaattcagtc cggcggcttt
     7261 aatttgaaga aagagtggtt aacgctggaa gaagtggtcg gtagcgcgct gcaaatgctg
     7321 gaaccgggtt tatcgtcgcc catcaatctt tctctgccag aaccgctgac cttaatccac
     7381 gttgacgggc cactctttga acgggtgctg attaatctgc tggagaacgc ggtgaaatat
     7441 gcgggtgcgc aggccgaaat tggtatcgat gcccacgttg agggcgaaaa tctacaactg
     7501 gatgtctggg ataacggccc cggtcttccg ccaggccagg agcagacgat atttgataag
     7561 tttgctcgcg ggaataaaga gtcggcagta ccgggggtag ggcttggact ggcaatttgt
     7621 cgggcgatag tggatgtaca cgggggcact attaccgcgt tcaaccgacc ggaaggtggt
     7681 gcctgttttc gtgttacact tccccagcaa actgcccctg aacttgaaga atttcatgag
     7741 gatatgtgac aaacgttctg attgttgaag atgaacaggc tattcgtcgc tttctgcgca
     7801 cggcgctgga gggcgacggg atgcgcgtct atgaggccga aacgctgcaa cgcggcttgc
     7861 tggaagcggc tacccgtaag ccagatttga ttattctcga tctcggcctg cccgatggtg
     7921 atgggattga gtttatccgc gacctgcgcc agtggagcgc ggtgccggtg attgtgcttt
     7981 ccgcacgcag cgaagagagc gacaaaatcg ccgcgctgga tgccggagcg gatgattatc
     8041 tgagtaagcc gtttggcatt ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc
     8101 actccgctac cgccgcgccc gatccgctgg tgaaattttc cgatgttacc gtcgatttag
     8161 ccgcccgcgt gattcaccgg ggtgaggaag aggtgcatct cacaccaatt gagttccacc
     8221 tgctggcggt gctgctcaac aatgccggaa aagtactcac ccagcgccag ctccttaacc
     8281 aggtgtgggg gccaaacgcg gtcgaacaca gtcactattt gcgtatttat atgggacatc
     8341 tgcgacaaaa actggaacag gatcccgccc gcccacgcca tttcattact gaaaccggta
     8401 ttggctatcg gtttatgctt tgaatattaa ttttaataca gcctgccttt tattaattaa
     8461 agccgtaata ataaatacgg ctttttatct taaacaacac acaaaaataa caattcaata
     8521 ttttatatta ctgagtaaaa agctcatcat taaataaatt aagattgatc attttttatt
     8581 gatcaccttc acagttcaac cgtatttcct ggatagaata tcttcacctt cattcacatc
     8641 aggaaaggta aattaaatgg aaaataacag ccgcactatg ccccatataa ggcggacaac
     8701 tcatattatg aagtttgctc atcgcaatag cttcgacttt cacttcttca atgcccgtta
     8761 gtctaccgac taagggcact tcagcgtaca ggtcttcctg actctctgta ttacaggcat
     8821 atgcctgtat tccgcagtgc tcattctctg ttaacccttg tattatcagc caccggactg
     8881 gtaagggttt ctgcattctg aaaaaagcaa tctacctgat ttatttatca gcggggtaac
     8941 tttgcttttt tccggcacga tcgatttctc attcgagaaa ttgaggacct gctattacct
     9001 aaaataaaga gatgaaaaat gtcaaaatta aaaattgcgg ttagtgattc ttgcccggac
     9061 tgttttacca cgcagcgaga atgtatctac attaatgaaa gtcgtaatat cgatgtggcg
     9121 gcaatcgttt tatcgctcaa cgatgttaca tgcggaaaac tcgatgaaat cgatgccacg
     9181 ggttatggca tcccggtatt tattgctact gaaaatcaag aacgtgtacc cgcagagtat
     9241 ttgccctgta tttcgggtgt ctttgagaat tgcgaatcgc gacgagaatt ttatggtcgt
     9301 cagttagaaa ccgctgccag ccattatgaa actcaactgc gcccaccttt cttccgcgca
     9361 ctggtcgatt atgtcaatca aggtaacagc gcgtttgatt gccctggtca tcagggcggc
     9421 gaatttttcc gtcgccatcc ggcggggaat cagtttgtgg aatactttgg tgaggcgctg
     9481 ttccgttccg acttgtgcaa cgcagacgta gcaatgggtg atctgctgat tcacgaaggc
     9541 gcgccatgca ttgcacagca acatgcggca aaagtgttca atgccgataa aacatacttc
     9601 gttctaaatg gcacttcatc ttctaacaaa gtggttttaa acgccctgct gacaccgggt
     9661 gatctggtgc tgtttgaccg caataaccac aaatctaacc accacggagc gttgctacag
     9721 gctggtgcaa caccggttta tctggaaacc gcacgtaacc cgtatggctt tatcggtggc
     9781 attgatgcgc actgttttga agaaagttac ctgcgtgagc tgatcgcgga agtcgcaccg
     9841 cagcggacaa aagaggctcg tcctttccgc ctcgccgtta ttcagctagg cacctacgac
     9901 ggtacgattt ataacgcccg ccaggtggtg gataaaattg gtcatctgtg tgactacatt
     9961 ctgtttgact ccgcatgggt cggctatgag caatttatcc cgatgatggc tgactgttca
    10021 ccgctgttgc tggatcttaa tgagaacgat ccgggcattc tggttacgca atctgtgcat
    10081 aaacagcagg ctggtttttc tcaaacttca caaatccata aaaaagacag ccacatcaaa
    10141 gggcaacagc gttatgtacc gcacaaacgc atgaacaacg cctttatgat gcatgcctcc
    10201 accagcccgt tctatccgct gtttgccgca ctggatatca acgccaaaat gcatgaaggt
    10261 gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg gcattaatgc ccgcaagctg
    10321 atcctcgata actgtcagca tattcgtccg ttcgtacctg aactagtgga tggtaaaccc
    10381 tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc gcttcttcca gtttgtacca
    10441 ggggaacact ggcactcttt tgaaggctat gcagagaatc aatactttgt cgatccatgc
    10501 aaactgttgc tgacaacccc aggtattgat gcacgtaacg gcgaatatga agcgttcggt
    10561 gtacccgcga cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc
    10621 gatcttaact ccatcctctt cctgctgact ccggcagaag atatggccaa acttcagcaa
    10681 cttgttgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa
    10741 gtgctgcctt ccatctacaa acagcatgaa gagcgctacg ccggttatac ccttcgtcag
    10801 ttatgccagg aaatgcatga tttgtatgcc cgtcacaacg tgaaacaacc gcaaaaagag
    10861 atgttccgta aggaacactt cccacgcgta agcatgaatc cacaagaagc caactacgcc
    10921 tattcacgcg gtgaagtgga gctggttcgt ctgccggatg ccgaaggccg tatcgctgcc
    10981 gaaggtgcgc ttccttatcc tccgggtgtg ctgtgtgttg ttccgggtga aatctggggt
    11041 ggt
//
LOCUS       DAJTSL010000136        10899 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTSL010000136 DAJTSL010000000
VERSION     DAJTSL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.136"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(228..2087)
                     /gene="gss"
                     /locus_tag="OO433_003182"
     CDS             complement(228..2087)
                     /gene="gss"
                     /locus_tag="OO433_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6051556.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2292..3158
                     /gene="yghU"
                     /locus_tag="OO433_003183"
     CDS             2292..3158
                     /gene="yghU"
                     /locus_tag="OO433_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6051557.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPICVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3281..3529)
                     /gene="hybG"
                     /locus_tag="OO433_003184"
     CDS             complement(3281..3529)
                     /gene="hybG"
                     /locus_tag="OO433_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6051558.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3542..3883)
                     /gene="hypA"
                     /locus_tag="OO433_003185"
     CDS             complement(3542..3883)
                     /gene="hypA"
                     /locus_tag="OO433_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6051559.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3876..4364)
                     /gene="hybE"
                     /locus_tag="OO433_003186"
     CDS             complement(3876..4364)
                     /gene="hybE"
                     /locus_tag="OO433_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6051560.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4357..4851)
                     /locus_tag="OO433_003187"
     CDS             complement(4357..4851)
                     /locus_tag="OO433_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6051561.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4851..6554)
                     /gene="hybC"
                     /locus_tag="OO433_003188"
     CDS             complement(4851..6554)
                     /gene="hybC"
                     /locus_tag="OO433_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6051562.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6551..7729)
                     /gene="hybB"
                     /locus_tag="OO433_003189"
     CDS             complement(6551..7729)
                     /gene="hybB"
                     /locus_tag="OO433_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6051563.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTREELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7719..8705)
                     /gene="hybA"
                     /locus_tag="OO433_003190"
     CDS             complement(7719..8705)
                     /gene="hybA"
                     /locus_tag="OO433_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6051564.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8708..9826)
                     /gene="hybO"
                     /locus_tag="OO433_003191"
     CDS             complement(8708..9826)
                     /gene="hybO"
                     /locus_tag="OO433_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6051565.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(10015..10302)
                     /locus_tag="OO433_003192"
     CDS             complement(10015..10302)
                     /locus_tag="OO433_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6051566.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10421..10899)
                     /locus_tag="OO433_003193"
                     /pseudo
     CDS             complement(10421..10899)
                     /locus_tag="OO433_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
BASE COUNT         2660 a         2991 c         2692 g         2556 t
ORIGIN      
        1 caaagtgaaa tatcattttt taacgataaa aagagtgtta tttcttgtgt atcagacaat
       61 aagcattcat tcaaaacctt tagaaataat caaaacacag gtattccact ggttaatata
      121 ttcacatatg aaatgaataa atattagaac atataaatat tgaatatttt gattaatccc
      181 cgtactgatt attcttcata atcagtacgg ggttgctaca acatgtatta ctttttcacc
      241 acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc accgcgcaaa
      301 cacgtcccac cgtagttgcc gcccacggtg aaggtacata cctgaatgta tttaccgtcc
      361 actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa tttaccgctg
      421 gttttgtcca gcacctcttc atgatggctg accaggtcga tattgctacc acagcgaccg
      481 gcaatcggtt tcactgcgta acctgttttc accaattcat cattaacagt gaaatcggta
      541 tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat cgctttgttg
      601 ccggggatca ccgtccacag cggctcaaag actagcactt ccgggcgcag caatacgtcg
      661 ataagacgca cttcgttttg cggatgaccg gtacggattg gcaccgcagc aaactcacgg
      721 tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt ccacacgcag
      781 ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc cagttcatcc
      841 aatccacgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc cataaactgc
      901 gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg acgtgcacga
      961 ctgtgtttcc aggcaccagc caattcgtta atcagccctt ccgccggatt gaagccgttg
     1021 cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt atgacaggag
     1081 gcggagtcgg cgttgtactc gtaaaccttc aggccacgct catccatgca gaaatccata
     1141 cgaccagtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg tggccagagg
     1201 attttcggaa tgtcgaacag cgccagcagg ttgtcatctt tcagtacctt gtcggttgcg
     1261 tgaagataca tcaggtgcag ctcgttggtg gctttaatta gctcctgctc ggcactctcg
     1321 gtaatggtgt agtaatgata aggatcctga ttgatcacct gaccgttggc ctgcacatag
     1381 gcgttttgca gcggatcttt ttcatccagc catttaccgt caaactggcc tttgttttcc
     1441 aggcgcgctc cgctgatttt cagcagctcg cctgcaattt ccggctgcgg taagctgtat
     1501 tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa agtgtctttc
     1561 agggtatagc agccgttttc gaccaccatc tccagctcac gcgtccactg ttgcccttgc
     1621 ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc atgcaattgg
     1681 gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca gataagaagc
     1741 gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg caggatgttg
     1801 tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat acccacgtca
     1861 gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac gcattgccac
     1921 ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc ttcgtattcc
     1981 tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg ggcgtagccc
     2041 aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac tttaccttta
     2101 acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct cagcgacatt
     2161 aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc aaagggttaa
     2221 aaaattcaca ttctgtacag caagtgacct gccacactgc ttcaacacta ccactcagaa
     2281 ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg tgggataaat
     2341 ccgctggcgg cgcgttcgcc aatatcaatc gcccggtttc tggtccgacg catgaaaaaa
     2401 cgctgcccgt tggcaaacac ccattgcaac tttattcgct gggaacgccg aacggtcaga
     2461 aagtaacgat tatgcttgag gagctgctgg cgctgggcgt tactggtgca gagtacgacg
     2521 cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc gaagtgaacc
     2581 caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc tgtgtgtttg
     2641 aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg ccgcaggatt
     2701 tggcaaagcg tactgaaacg atgaactggc tgttctggtt acagggcgcg gcaccgttcc
     2761 tcggcggtgg ttttggtcac ttttaccatt acgcaccggt aaagattgag tacgccatca
     2821 accgctttac catggaagcc aaacgtctgc tcgacgtgct ggataagcaa ctggcgcagc
     2881 ataagtttgt tgcgggcgat gagtacacca ttgcggatat ggcgatttgg ccgtggtttg
     2941 gcaacgtggt gttaggtggt gtgtatgatg ccgctgagtt tcttgatgcg ggcagttata
     3001 agcatgtaca acgctgggcg aaagaagtag gcgaacgtcc ggcggtgaaa cgtgggcgta
     3061 ttgttaaccg caccaacgga ccgctgaatg agcagttgca tgagcgccat gacgccagtg
     3121 atttcgagac gaatacggaa gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa
     3181 gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca atgcctgatg
     3241 cgacgctgcc gcgtcttatc aggcctacga aagctaatca tcacgcgctg gtaatgtcgt
     3301 aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg atgatgctca
     3361 tggcaaatcc gacgtgtacc agcacccact ggcccagtag atcggcaggg ttaccttcac
     3421 aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc gcaagctggt
     3481 gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt attaactccg
     3541 gttattcaac ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct
     3601 cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat
     3661 cccagcacca ggcctgggcg ggtttataga cgatatgtaa atcgcaccct tgcgccaccg
     3721 ttccgtggca gacaatttca aaactaaaac ggacggcgct ctcctcaacg caggagagcg
     3781 cgccaatttc cagccacacg gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt
     3841 ggataatttc aacggcgctc tggcaaagcg acaactcatg cattttcgcc actcctgcga
     3901 ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg gattcgtgac tggcagcgaa
     3961 aggatcattc gtgcgcagtc atccgtcagg cgttggccct cttcaatcga catactgtgc
     4021 gaaagcggtg acatcagcga acaggagagg tattgcgaaa caccgtccag ttcgccaacg
     4081 gtaaaggtca tggtgccata cggcagttgc agaccaattt tttcactcac tttgcgcagc
     4141 ggccagagtt gatccgggcc ggggaagata actgcactca gcatccacgg ggtgatcaca
     4201 caccccgtcc actgaccttc gaacagcgta aaatcagaaa catacaccgg cattgaagga
     4261 tgcagaaaag agagatcgtg catcgaacgc cgggcaattt cttcaaacgc tgcctgtact
     4321 tgcgccttcg gggaggtctg gaaacctgct atctcttcag tcatgaatcg cctcccgtgg
     4381 gatggcttcc acgcctgatt cgcgcagcgc agccagaacc tgctcaagcg caggttcaat
     4441 cattgcttca accgtcggcg ttaagccgat gtgtggctcc agcgattccg ggatcacgcc
     4501 gaccagggtc agctttttcg gaaactcacc ggtgaagcgc agggccgaca atacgtcggc
     4561 caggccaagc tgatgcggag agattttgtt ggtaaacaac gccggaactt cttcatcccg
     4621 caggatcatc atcgttcccg gcgtgttctt tttcgacaca atggcatccg caataatcag
     4681 atgatcgcga tttgccatgt cgccaagcag ctccattccc gccgtgccgc catcgaggat
     4741 ctcaacataa tccggcagaa tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat
     4801 ggcttcatcg gtcagcaaaa tattgccgac ccctaagact aaaatacgca ttacagaacc
     4861 ttcactgaaa ccacttcgtt gccgtcggca tccactacgt gtaccgcaca ggccatgcac
     4921 gggtcgaagg agtgaatggt acgcaccact tccagcggtt tattcggatc agcaaccggt
     4981 gtacccacca gcgactgctc gtaaggaccg acgtcatcat tgaagttacg cggaccagag
     5041 ttccaggttg atggaacaac tgcctggtag ttactgataa taccgtcttt aataaccatc
     5101 cagtgagaga gcataccgcg cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt
     5161 gccggaatgt tcggtttcac aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt
     5221 gcactgtatt gattttgcag gatatcctgc aattcgcagc agtgaacggt acgaccaata
     5281 atacggccca gcgtggagtg cagctgcgcc acttccagcg tgttgccagt cagtttctga
     5341 taaatcgcaa cgatttcatt cagtttgttc tgggtagatt cgcgacctgc cgccagtttc
     5401 accagcatat tagccagcgg acccacttct accgttttgc cgtagaaagt cggtgatttc
     5461 acccaggaat atttcccgtc gtcagaccaa ccatcataag ccggaatggt ggtaccttcc
     5521 cacggtgcct gcggcgcttc gtctttatac caggagtgct tcgcgctttc ctgaatgcct
     5581 ttgatcaggt attcatcgga atgagaagtg atcggacgat acgaggacag atccgcattc
     5641 tcaatgtagc cgcccgggaa caggaagctg ccgtttttac tgtcggtcgg gaattccggc
     5701 acgctcaggt agttcaccgc acctttaccg cgcgtcagcc attccgggta gaacgcggca
     5761 ataactgcgg tatcaacctt ataaacctgt tcaacaaagt cgctcagctt gtcgatgaaa
     5821 gacttgatgt acatcaggcg ctcaaggttc agcacgccca aaccgtcgag gttgatcggg
     5881 ttcgcgacac cacctaccgc caggttctga atgtgcggcg ttttaccgcc cagcagcgcc
     5941 acgacgcggt tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc
     6001 aggttcactt ccggcggcag tttcatcgcc gggtgacccc agtagccatt ggcgaaaata
     6061 cccaactgac cgctggcaac cagatctttg atcttgttct gaactttagt gaactcttcc
     6121 ggactgttca gatgccaggt cgaaacgcct ttcagcattt cggaggcttt ggttgggtca
     6181 gcttgcagtg cagaagtgat gtccacccag tccagcgccg aaagctgata gaaatgaacg
     6241 atatggtcat gcgtggtgtg cgcagccaga atgatgttac ggatgtattg cgcgttaacc
     6301 ggaacgtcga tattcagcgc actttctgcc gcacgaacgg aagacagcgc gtgagtggta
     6361 gtacatacgc cacagatacg ttgcacaatc atccatgcat cgcgcggatc gcggtttttc
     6421 acgatctctt ccatgccgcg ccacatggta ccggaagccc atgctttcga aacgacgcca
     6481 ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg atcaatagta
     6541 attctctggc tcatgctttg ctcgcctcat gacgattatg atcgttttgt tttaaaggag
     6601 gaagtatcgg cagtagacga atgagtacga tgtaagcgca aatctcaata gccacaaaac
     6661 caatagaaat caacagttct tccctggtcg ggaagtaggc gtaaccgccg cccgggttga
     6721 atgccaccag cgaataggtc agacgccagg ttgcacaacc taacagtgcg ctcagtgctg
     6781 acaggaacag catgcgggaa tcattacgca gcttcgccac acgcagaacg accagcggga
     6841 agagcatcag caggacttca atccagaaca tcacggagta gaagtcaccg gcaaacgcta
     6901 acgacagctt gtcgcgatag ataagctcgc caaagcgcag cacgataaaa atcgccagca
     6961 acacactgat ggtgttggtc agcttgacga acagactctt ttcatccgga ccgttgccac
     7021 gcagacccgc ctgcaccagc gaaccttcaa agatgacaat cgagaagccc atgatgaacg
     7081 ccgtcagcag cgagaacagc ggcaacattt catagctctg ccacaacgga tgcaccttgt
     7141 agcccgccga gatcatcagt gaccccattg aagactggtg catggtcggc aacagcgcgc
     7201 caagtgcaat gatgaagaac atcaccttgt tcagacgctt gagagaaacc ttccagccca
     7261 gacgttcaaa cagtgccgga gcaaactcca gtgccatcac gccgatatag atggtcatac
     7321 agaccgccgt ctcgaacagt accgagttca cgttgaagtg acccggaatg tagaagtacg
     7381 gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa gccacccagt gagtaaccaa
     7441 acagactcgc caacagcgcc ggacgcacca gcggatggta ttgcccacgg ttaaagacgt
     7501 ataccgccca cgccagcgcc cagccgccac aggcaaagcc ggtaccaatc agcaggtcaa
     7561 acgcgatcca cacgccccac gggaagccgc cgttcaggtc agagacagag cccagaccga
     7621 acaccagacg cttcacaatc aggagcatac agatgacgat taacggtcca aaaatcatga
     7681 ccggtttact gatgattttg ccgcccagcg gttgtggatc atgactcatg atcgtctcct
     7741 ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca cggttaagcc cgccagcaca
     7801 gccagtggta gcatcatgcc tttatacagg gtgtgttgaa tattttcgga acgcgcaccg
     7861 gtagaaagat cgtccagttt cggcaggtcg agattttcat aaggcacacc cgtcagtacc
     7921 agaacctgag taccgccgcc ctctttctcg ccgtacagat gcggataata tttcggcacc
     7981 gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg gatagtggta ttcgctgcca
     8041 ggcttcagcg ccagacgttt tttcgcctcc gccatcagct cttcacgcgt accgaaaatc
     8101 accgcgcccg ccgggcacac ttctacgcag ccaggcagac cgcctttatc gagacgttcc
     8161 acacctttct ggttgcacag ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg
     8221 tacttcggca cgttgtacgg acaggcgacc atgcagtaac ggcaaccggt gcacacgtct
     8281 ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca gtgcagagac cgggcacaca
     8341 gagacacagt tcggatcgac gcagtgcata cactgtttct taatgtacgc gtagccgttc
     8401 tcctcctggt ctttgttgac ccctgtgccg ctggtccaca cctgaatgat gttattggta
     8461 tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt acgttcaggg
     8521 aagttgatat cctgacactt ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg
     8581 tacaacatcc ccagcgatcc cggaattggc gggcggtttt cagcagccgc atgactgaca
     8641 gacggcagcg cgcccgtcag caatgccccg caggaggctg ctttaataaa attacgtctg
     8701 ttcacggtta ttctccccgt gagtcagcgt tatctttctt ttgctgacga cccagttcac
     8761 gcaccgccat cacgctgaca ccggcaacca gcccaaccac accgccgagc aaaccaatag
     8821 cgcctgcaga gacgttaccg ccctctttag cgttaacatc cggtttctgt gaacgcggag
     8881 tctgattttc gacgttggca agctgatgga tgcctttatg gaagccgata ccttcttcgt
     8941 tacagccata gcatgggtga ccaatcgcta ccggccacac gccgccaaca tcgcagaatt
     9001 gcagcgttga gcagttgccg taagtttctg gccctttaca gccgaggtgg tacaggcacc
     9061 agccttcacg gtggccttca tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg
     9121 ggcgacgttc gcagtgttcg tgaatcagac ggccataggc gaaggtcgga cgatttttgt
     9181 catccagttt cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt
     9241 gcgggttcgg cgggcagccc ggaatattga taacggtttt gcctggcaga acttcttgca
     9301 ggctgactgc gccagttggg ttaactccag ctgcggcaac accgccccac gcagagcagg
     9361 aaccgatagc gataatcgct gctgcgcctt ctgccgcttt gcggatgtga tccacaatcg
     9421 gctcaccagc aaccatgcaa taaataccgt tatcttttaa tgggatggaa ccatccacaa
     9481 ccaacacata ctgccctttg tacttctcaa gtgcgttatg tttgttctct tcgacctgat
     9541 gaccgaaggc ggcggaaagc acttcgtgat actccagaga gatagtctcc agcacgaggt
     9601 tttctaccgt tggatgcgtt gcacgaagca gagattccgt acaaccggtg cactcctgcg
     9661 cgccaatcca gataactggc ggacgctgcg ggttagtaac cgattcggcc atctctgcag
     9721 cggctttgct acttaacccc atggtggcgg ctaatgctgc acaaagcttc atgaaatcac
     9781 gacggttaat gccgtgagaa tggatgaggg tgttatctcc agtcatttat agttattccg
     9841 ttgcgaagac ctggcatata ttttgcctca atcgcaaaat caataatgcg atcgatgcgc
     9901 catttaccac acatttatta tggttatcga tatcatgata ctgcggcggg gcgctaaaac
     9961 gaagggaaga agcattagtg taattaatta aagcaagata atacgtatgt ttgatcaatt
    10021 ttcgaggcga taaccggcca taaaaaagcg caacgtagaa caggaattat tctcacgaaa
    10081 gaaatccatt accatctctt tatccagtcc atagcggcgc gtcagaatac ccgcttccca
    10141 ggcgctaagc tggcgatctc cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa
    10201 accgcgttta taatcggcac agaattttgt cacattaacc gcactgtcgg catgcgttgc
    10261 ttttaatccc gccattaagc ctttaccaaa atggttattc atcgttcacc tcattcgcta
    10321 tatattatca tatatagcaa tattttaggc aaagaacgac ttttgcgcac gctatttttt
    10381 catgcttgtt taagacgtaa tcccgtaaga gataaaccca tcaggagaga tatttatcga
    10441 accatttcag cgtcctttgc caggcaagat cggcggcaga tttgtcataa cgaggcgtgg
    10501 aatcattatg gaatccgtga ttaacccccg gatagatata cgcctcatag accttattat
    10561 tggctttcaa cgccgcctca taagcgggcc agccctcgtt gattcgggtg tccagttccg
    10621 cgaagtggag tagtaaaggc gcttcaatct tcgccacatc ggcagtgggt gcctgacgac
    10681 cataaaacgg caccgcgcag gccagttccg gatacgcgac agccgccgcg ttcgatacac
    10741 caccgccata gcaaaatccg gtaataccca cttatgcctg tcgcttgcgg atagcgttgc
    10801 ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac ctgctgttgc
    10861 agctcacgac ctttatcatc atttcccgga taacctcca
//
LOCUS       DAJTSL010000137        10849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTSL010000137 DAJTSL010000000
VERSION     DAJTSL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.137"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..853)
                     /locus_tag="OO433_003194"
                     /pseudo
     CDS             complement(1..853)
                     /locus_tag="OO433_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(456..572)
                     /locus_tag="OO433_003194"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(907..3017)
                     /gene="fdeC"
                     /locus_tag="OO433_003195"
                     /pseudo
     CDS             complement(907..3017)
                     /gene="fdeC"
                     /locus_tag="OO433_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
     gene            4133..4234
                     /locus_tag="OO433_003196"
     CDS             4133..4234
                     /locus_tag="OO433_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051567.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            4598..4861
                     /locus_tag="OO433_003197"
     CDS             4598..4861
                     /locus_tag="OO433_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR6051568.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            4861..5001
                     /gene="ykgO"
                     /locus_tag="OO433_003198"
     CDS             4861..5001
                     /gene="ykgO"
                     /locus_tag="OO433_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCR6051569.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(5036..5263)
                     /locus_tag="OO433_003199"
                     /pseudo
     CDS             complement(5036..5263)
                     /locus_tag="OO433_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6086..6628
                     /gene="ecpR"
                     /locus_tag="OO433_003200"
                     /gene_synonym="matA"
     CDS             6086..6628
                     /gene="ecpR"
                     /locus_tag="OO433_003200"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HCR6051570.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINCRFLRKDIKKDKITDQEMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            6654..7289
                     /gene="ecpA"
                     /locus_tag="OO433_003201"
                     /gene_synonym="matB"
     CDS             6654..7289
                     /gene="ecpA"
                     /locus_tag="OO433_003201"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HCR6051571.1"
                     /translation="MTHWDITSSIQLGKKCNEKKVLAIALVTVFTGMGVAQAADVTAQ
                     AVATWSATAKKDTTSNLVVTSLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKL
                     TSRLITNTLTQLDTSGSTLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYN
                     ASNRTTAQDGFTFTIISGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            7347..8015
                     /gene="ecpB"
                     /locus_tag="OO433_003202"
                     /gene_synonym="yagY"
     CDS             7347..8015
                     /gene="ecpB"
                     /locus_tag="OO433_003202"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HCR6051572.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            8041..10564
                     /gene="ecpC"
                     /locus_tag="OO433_003203"
                     /gene_synonym="yagX"
                     /pseudo
     CDS             8041..10564
                     /gene="ecpC"
                     /locus_tag="OO433_003203"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
     gene            10554..>10849
                     /locus_tag="OO433_003204"
     CDS             10554..>10849
                     /locus_tag="OO433_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051573.1"
                     /translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNW"
BASE COUNT         2881 a         2733 c         2531 g         2704 t
ORIGIN      
        1 gcatccagca cgaatgtcgt ttccatggac gatgagaccc gccaccccac gatgtatccg
       61 gcaaacacat caataatgaa cgccacatag acgaagccct gccatgtgct gacgtaagta
      121 aaatcagcca cccacagctg gtcaggtcgt tctgccacga actgacggtt tacgcggtcg
      181 cctgcggcaa cggctttccg gctgatggtc gtacggacct ttttaccccg gagaacaccg
      241 gcaagtccca taaccgccat gagacgtgcc acagtgcatc tggccactct gataccttcc
      301 cgtaacaact gacgccagac tttacgcaca ccgtatacct gatgattccc atcgtatacg
      361 cgcagtatct ctttcttcag ccagtcatcg cgctgcgcac gggcactgcg tttatcagga
      421 tgatgtcgct gttgctgaca gtggtaatac gttgacgggg caatatgtag ttcactgcat
      481 accggtccga ccccgtacag cttacgcagc ttatccagca gtggcatcac tttttccaga
      541 ggcggtcgaa ctccgccttc gcaaaataag cggaagcctg gcgaaggata tcgttactgc
      601 ggcgcagttc acgattttca cgttccagct ctttcagacg ctgacgttca gcggtggtga
      661 gcccgccatc accgcctccg gtatcccgct catgctggcg tacccagaca cgcagagtct
      721 ccggtgtaca gccaatcttt ggggcaatgg aacaaattgc cgcccactgt gagtcatatt
      781 cgccctgact ttccagaacc atacgaatcg ccctctgacg gacttcgggg gaaaaacgag
      841 tatttttagt catcctgttt acctctttct cagggagttt agtctccagg attcccgggg
      901 cggttcaccc ccaaccaccg gtttatcagg attcagggtg atggacgaat gtgctgcatc
      961 aagcggcccg gcaacaaact tcagcgtttg ttgtaatgtg cccagctgat actgctcgct
     1021 gaaagcacgc acacacgcac aacaacgttt ccggcacggg tcgaagaaac cgtagcgctg
     1081 taaacgcctg gttttatctc cgaaattgta ccaatggtga caccattagt gtcttgcgga
     1141 acaaatcgca agcggctggc ctctcccgtc accgggttac catcagtatc caccgcagta
     1201 agcgtcagcg tgtgggattg ctgaccatca gcaaccacat ctcctgagag ttcattcacg
     1261 ctcagagtag acttcgacac atccatcatc gtggcgcgca attctgcagt gacggttttg
     1321 ctcatgccat caacgctaac agtaatcgtt gcctcacctg attgcgttcc ggtagtgaag
     1381 acagactgat acaccccagc ttcagtttca gtgaactcac ccagtgttgg ctttgcctgt
     1441 gatttagtgg ccttcaggga acgagtcaca atatttccag ccggtttgaa agttagttca
     1501 gtcttgatct gatctttcat gcccgtgact ggctggccct cggcgtcgcg cagagacagc
     1561 accagcggtt tttgctcatt accgttagca agcatttgaa tacggctctg accgtcaagc
     1621 gttaacgccg tgcgatcggc gctcatacca gctccggtaa tgaccacctc tgtctgcacg
     1681 cgttttgagg tattgccttt gttatcgtag gcaaccgcag aaatcgcata ataattgtct
     1741 ttgcctggac gataagccgg gagcgttact tgccactgac taccctgacc ggtaattttg
     1801 ccaccttcag ccagtaatga cggcgcttcc cactgcacat ttttcagtcc gtgagttgct
     1861 ttgctgacca caagcccgag ggaaagtgtc tgacagccct tgccttcaat acgctcaggc
     1921 agagcaatac ggatcacttc agatttgcgg tactcaagaa cgatgttgtt attacgctca
     1981 accaggtcat agcggctgcc tgctagcatt cgacgctcgc gaatgctgtc tgtatcgagt
     2041 tgtttctcca gaggttcgcc aatccgataa ttaacttcca ggccaaagcg agtgtcattc
     2101 tcaccactct tgccctgctt atgcccggca ctcagggtca gaagaggcac aggcgtgtaa
     2161 ttcacttcag cggaaatcgc atgtggatct ttctggcgtt tatctttgcc aaacagccca
     2221 acttcatcgc cataatactg ctcatacatc aggcttgcgc caagctgcgg tcaggcgggt
     2281 aaatagccct cagcacgaat atcccagcca ttcgccgggc gttcctgata atcctcaata
     2341 tccggcgatt ttttccagcc agaagcccga atataaccat tggcgctcag tttcagataa
     2401 tcgcgccagt attccgcacc aacaccaatg cgggtatgac tacgggataa atcatggtcg
     2461 ataaaggtgt tcaccccccc cattcagtca tttcctgaaa aatgacgcca gccaaaacca
     2521 atatttgact gagtacgatc gtctgtacga tgtattgccc cctgagtgaa caacatattt
     2581 gtcggcgtat cataaatcgg ataaagcatt tccagcgaag aatccttcag cgagaaatct
     2641 ttatcgacat tcagtttgac gcgcgctgta ccatatttcc cgagccactc ctgtatttcc
     2701 tggttagctt tagcggtggc cattccggta ataaaattac gtgtcgcatc gctatctggc
     2761 tgactgctta aaaatgtccc ggcatttgcg gcaaacgacg cgacattttt ctccacgtta
     2821 ttatcagcag ttaccgtagt atttcccatg ctcaaccgtg gctgaaccgc atgctgcgca
     2881 cgtgccgcca ttactggggt aaaggtgaca gcgagtggaa aaagaacctg aacagagata
     2941 tttgcccacg ccacgcagcg ggccagaact gaataacgaa atcgtggttg tttatgacct
     3001 gttttataac gtgacattga gataatttcc tgatgaacga attaacgaac ataggcagac
     3061 acaaataatg gcgtctgctt gtcagaaaaa ccggggattt cccggtttcc ttttccgcag
     3121 aaaaggaaat acctttatgt aagaaagtgc accgccacct gatatatcca gtgtctgtag
     3181 ccactcatca ggtggttggg cgcgggatta tatttttatt tatgggtaaa aatttagctt
     3241 tttgtgcaag tcattaaata gatacctaag ttatatttct tactctgcgg acagtgaata
     3301 attcattatt cagactgaat aataatgacc tgcctttaat tagcaactat tggaaatacg
     3361 tcgaagtagg tatatacgcc aggataaacc gatatattta ttatggatat tcattttcct
     3421 tcatcggact gtaaattgcg gggatatcga acttacacta ctcatcgcaa cactcaaaca
     3481 gatttatgaa tttcgttcat aacccctgac aaatcctgcc gtctaatcga gcttttccct
     3541 ttacgttatg cttctcatga acgagaacac aacttattca tgaattaacc attccggata
     3601 aactatggaa tcaaattcag gcatcgaaag gaactggctg gtttccgtta atagattaaa
     3661 tatctattac tctcccgaga gtaaaactat caaatcaatg agtaaccgat acagcctcac
     3721 aaattctttc agataattaa cccgtcactg cggcacgttc tctcgtaagg cgtgcctgca
     3781 gtgtaatcca gtatgtatcc ggcatagttg acggtttaat agaactcaat tcctctccct
     3841 ggtcaccaac aacgatatct tgtataacac cccaggagat acgacattat gatgagatgc
     3901 gatatgctcc tcagcaccaa tcaacgccag gactgggcca gtcttgagat cagactcatt
     3961 cccgggactg acttcagtcg acatatatca atgacgttgg tgcaataaaa gatagccgat
     4021 gtaatacatc tattcaaatt tgtgatacat gtcaaaaaac tggttgacca aactcgttat
     4081 ttatataagg gcacttacga agtgcgctct tttttaaagc gaggaagtac caatgaaaga
     4141 gaataaagta caacaaatca gtcataaact aattaatatc gttgtttttg tcgcaattgt
     4201 agaatacgcc tatttatttc tccatttcta ttaataacgg caataaactg ttcacttcag
     4261 tgatatttaa aatatgcatt ctctcccttt ttgtaagtaa ttattatatc cgtgggagag
     4321 gaatacacgc tgtcaggtaa tcaatcatac tgcgataaat catcggccag taaagtggag
     4381 ataacctcca ttgtcgaaaa atccatactc tcagcgaaac catcatcaat cactcatcca
     4441 ggcgtttatg ggagcgtcgc caacggctgt taacaatgcc agacttcccc gttgcggaaa
     4501 ttccacatac cactaatggt cacagggatt gggtgttgaa atgatccgga tgagcatgta
     4561 tctttatggt tatgctataa cataacaggt aaaaatgatg aagcccaata tccatcctga
     4621 gtatcgtact gtggtgttcc acgacaccag tgttgatgag tactttaaaa tcggctcgac
     4681 tatcaaaaca gaccgtgaga ttgagctgga tggcgtaacg tatccgtacg tgacaattga
     4741 tgtctcttct aaatcgcacc cgttctatac agggaagctg agaacagtgg catcagaagg
     4801 aaatgttgca cgattcaccc aacgttttgg tcgttttgtt agcacgaaaa aggggtcgtg
     4861 atgaaagttc ttaactctct gcgtaccgca aaagaacgcc atccagactg tcagattgtg
     4921 aagcgaaaag gacggctata tgtgatttgt aaatctaatc cacgttttaa ggccgttcag
     4981 ggtcgtaaga aaaaacgttg attcaaaatt cgacggatta acgatatttg tctgattaat
     5041 aatcagatcg gattaatgtt ggtgtgttta taacaccaac attaattttc ctggggatat
     5101 attcttcctg ttcatttgag gccaactgcc tgacgtttct ctacgaatat tccattatct
     5161 taatgttgac ttgttgacca gcttcgcccc tgtatgctgg catcaactct cttttagact
     5221 gaacacgcca ctcagtctcc tccctttgcg gcgcagcctg cattttcact caaactgtta
     5281 agatgataaa tgtggtaaat ctgttggtac taacataaaa acgtttacgc cacaggaaca
     5341 gtctgatcca ccggtaaccc cgtcgccgac gttcgagtgc cagttagagt aacgcgcaca
     5401 gataactgca ctcagtgccc tgacaaagag gccatcgttc ctgtgacagc tggcagcctt
     5461 cgtttaactt cacttaatct ggctcttgag ggcttaccga acagatgacg tacatacgcc
     5521 cgttcaattt tccatcactt attgtaatga acacctataa ccattttgtg cggcatgtta
     5581 atccattaaa atttcttact gattggcaaa tcatcttcaa tgacagctca ttatagtttt
     5641 atattctatc ccttaccctt aaaacttgtt tttttactag tccatcacac agcgcattaa
     5701 gactattcct aacacttcag ggcaaagttc ctgaccaata taaaatgcaa gtaagaattg
     5761 aacgttatat tgccaataac cttatgaaac caaatgtctt tttcttctta tcaaaaaaac
     5821 aatattttca gtttttctaa atattgactt aaccattgaa ttccttttcc gttcacatat
     5881 tgacactcat cgaaaaaaaa acataaattt aagcccaatc gaaaataatt aaacttaatc
     5941 tcgtttaacc tttattgata tgtactacgt atcttattta cttccggttt actaaggaaa
     6001 ctgaatgcac ctgtaaaaat tacaggtttg gaaagtagtg acatggcaaa gtgattacag
     6061 tagggactat gaggttaaaa accatatgga atgtcaaaac cgttctgata aatacatctg
     6121 gtctccccat gacgcctact tctataaagg actatctgaa ctgattgtgg atatcgacag
     6181 attaatttat ctatcgttgg agaaaattag aaaagatttc gtgtttatca atctcaatac
     6241 ggattcttta tctgaattta taaaccgtga taatgaatgg ttatccgcgg taaaggggaa
     6301 acaggtcgta ttgattgcgg ccagaaagtc agaagcctta gcaaattatt ggtactacaa
     6361 cagcaatatt aggggcgtgg tatacgctgg actgagtcgt gatattagaa aagaactggc
     6421 ctatgtgatt aattgcaggt tcctgagaaa agatattaag aaagataaaa tcacggacca
     6481 ggaaatggaa attatccgca tgacggccca gggaatgcaa cctaaatcga ttgccagaat
     6541 tgaaaattgt agtgtgaaga cagtgtatac ccatcggcgt aatgctgagg ccaagctgta
     6601 ctcaaaaata tataagttgg ttcagtaaac tccaggcaag ttagttttaa aaaatgactc
     6661 actgggacat cacgtcctca attcaactcg ggaagaaatg caatgaaaaa aaggttctgg
     6721 caatagctct ggtaacggtg tttaccggca tgggtgtggc gcaggctgct gacgtaacag
     6781 ctcaggctgt agcgacctgg tcggcaacag ccaaaaaaga caccaccagt aatctggttg
     6841 tgacgtcact cggtagcttg gcgttccagt atgccgaagg cattaaaggt tttaactcac
     6901 agaaaggtct atttgacgtg gctatcgagg gtgactcaac ggctaccgcc tttaaactga
     6961 cctcacgtct tatcaccaac acattaactc agttggatac ctcaggttcc acactgaatg
     7021 tgggcgtgga ttataacggc gcggcagtcg aaaaaactgg cgataccgtg atgatcgata
     7081 ccgccaacgg cgtactgggc ggcaacctta gcccgctggc taacggttac aatgccagca
     7141 atcgtaccac cgcacaggat ggtttcacct tcaccatcat cagcggcacc accaatggta
     7201 ccaccgcagt aaccgattac agcactctac cggaaggcat ctggagcggc gacgttagcg
     7261 tacagttcga cgcgacctgg accagttaat ctctctgatg taccagcagg ggtagccccc
     7321 ctgctttatt cctggacgga attgttatga aaaagcacct tctgcctctc gctctgctgt
     7381 tttccggaat atctccggcc caggcgctgg atgtcggcga tatatcatcg tttatgaaca
     7441 gtgacagcag cacgctgagc aaaacgatca aaaacagtac cgacagtggt cgccttatca
     7501 atatccgtct cgaacggctc tcttcaccgc ttgacgacgg gcaggttatc tcaatggaca
     7561 agccggatga gttgctactc actcccgcca gcttgctgct acccgcccaa gccagcgaag
     7621 tgatccgctt cttctataag ggacccgcag atgaaaaaga gcgctactac cgcattgtct
     7681 ggtttgatca ggccctcagt gatgcgcagc gcgataatgc caaccgcagc gctgtggcca
     7741 ctgcttccgc ccgcatcggc accattctgg tcgtcgcccc tcgtcaggcg aactaccact
     7801 ttcagtacgc caacggctcc ctgacaaata caggaaatgc gacgctgcgg atcctcgcct
     7861 acggcccctg cctgaaagcc gccaacggta aggagtgtaa agagaattac tacctgatgc
     7921 cgggcaagtc gcgtcgtttt acccgcgtgg acactgcgga taacaaagga cgggttgcac
     7981 tttggcaggg tgataagttc attcccgtga aatagatagc tgtgcagatg gataacgaca
     8041 atgcctttac gacggttctc cccaggactg aaagcccagt ttgccttcgg catggtcttt
     8101 ttgttcgttc agcccgatgc cagcgctgct gacataagtg cgcagcaaat aggtggggtg
     8161 attattccgc aggccttcag tcaggcgctt caggacggca tgagcgtccc gctctatatt
     8221 catctcgccg gtagtcaggg tcgccaggac gatcagcgaa tcggcagcgc ttttatctgg
     8281 ctggatgatg gacagctacg catccggaaa atacagctgg aagagagtga agataacgcc
     8341 agtgtcagcg aacaaactcg acagcagctg atggctctgg cgaacgcccc gttcaatgag
     8401 gcccttacca tcccctgact gacaacgcgc agttggatct cagcttgcgc caactgctgc
     8461 tgcagctggt ggtcaagcgc gaagcgctgg gcaccgtact acgctcacgt agcgaagaca
     8521 tcgggcagtc cagtgttaac accctcagca gtaatctgag ctataacttg ggcgtctata
     8581 acaaccagtt gcgtaacggc gggagcaaca catccagcta tctgtcgctg aataacgtta
     8641 ctgcgctgcg cgaacatcat gtggtgctcg acggctcgct gtacgggatc ggtagcggtc
     8701 aacaggacag tgaattatat aaagcgatgt atgaacgcga ttttgccggt caccgatttg
     8761 ccggtggaat gctcgacacc tggaacttgc agtcattagg gccgatgacc gccatttcag
     8821 cagggaagat ttacggcctt tcctggggaa accaggccag ctccaccatc ttcgacagca
     8881 gccagtcagc cacgccagtg atcgcctttt taccggcggc gggcgaagta catctcaccc
     8941 gtgatgggcg gctactaagc gttcagaact tcactatggg caatcatgaa gtggataccc
     9001 ggggtctacc gtacggtatt tacgatgtgg aagttgaggt gatcgttaac ggtcgcgtga
     9061 tcagcaaacg cactcaacgg gtcaataagc tgtttagccg gggcgcggcg tcggtgcacc
     9121 actggcgtgg caggtatggg gcggtagctt tcatatggat cgctggtcgg aaaacgggaa
     9181 aaagacgcga ccagctaaag agagttggct agcaggtgcc tcgacctccg gctcattgag
     9241 tacgcttagc tgggcggcaa cgggatatgg atacgataat caggcggtgg gtgaaacccg
     9301 tctgacgctg ccgcttgggg gggcgatcaa cgttaacctg caaaatatgc tggccagtga
     9361 cagctcatgg agcagcatcg gcagcatcag cgctactcta ccgggaggct ttagttcgct
     9421 gtgggttaac caggaaaaaa cccgtattgg caatcaattg cgacgtagcg atgccgacaa
     9481 ccgtgcaatc ggcggcacac tcaacctgaa ctcactgtgg tcgaagctgg gcacattcag
     9541 catcagctac aatgatgacc gccgttacaa cagccattat tacacggcag attactatca
     9601 aaatgtctac agcggtacct ttggttcgct tggcctgcgg gccggtattc agcgctataa
     9661 caacggcgac agcaacgcca atacagggaa atatatcgct ctcgatctct cgctaccact
     9721 gggcaactgg tttagcgcag ggatgaccca tcaaaacggc tacaccatgg caaacctgtc
     9781 agcacgcaaa cagtttgatg aagggaccat tcgcactgtt ggtgccaatc tgtcacgtgc
     9841 catctccggc gataccggtg atgacaaaac cctcagcggt ggggcgtatg cacagttcga
     9901 cgctcgttac gccagcggaa cgctgaacgt caatagcgcg gcggacggct acgtcaatac
     9961 caacttgacc gccaatggca gcgtcggctg gcagggtaaa aacattgctg ccagcgggcg
    10021 gactaatggc aacgctgggg tgatattcaa caccgggctg gaggacgacg gtcagatcag
    10081 cgccaaaatc aacgggcgga ttttcccgct taacggcaag cgtaactatc tcccgctctc
    10141 tccctatgga agatatgagg tggagttaca gaacagcaaa aactcactcg acagttacga
    10201 tatcgtcagc ggccgcaaaa gtcgtctgac tctctatcca ggcaatgtcg ctgtcattga
    10261 gccagaggtg aagcagatgg ttaccgtctc cggtcgtatc cgtgcggaag acggcacact
    10321 gctggctaac gcacggatta acaaccatat cggccgaacc cgaaccgatg aaaacggcga
    10381 gtttgtcatg gacgtggata agaaataccc cactatcgat tttcgctaca gtggcaataa
    10441 aacctgcgaa gtggctctgg aactcaacca ggcgcgcggt gccgtctggg tcggtgatgt
    10501 ggtctgcagc ggcctctcat cgtgggcggc ggtgacgcag acaggagaag agaatgagag
    10561 ttaacctact aataacgatg ataattttcg cgctaatctg gccagtaact gcgctcagag
    10621 cggcagtgag caaaacaacc tgggcggatg caccggcacg cgagtttgtg tttgtcgaaa
    10681 acaactcaga cgacaacttt ttcgtcactc ctggcggggc gctggatccg cgcctgaccg
    10741 gtgccaaccg ctggaccggt ttaaaataca ctggttcagg aaccatctat cagcaaagcc
    10801 tcggctacat tgataacggt tacaacaccg gcctttatac caactggaa
//
LOCUS       DAJTSL010000138        10820 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTSL010000138 DAJTSL010000000
VERSION     DAJTSL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.138"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..699
                     /gene="hdhA"
                     /locus_tag="OO433_003205"
                     /pseudo
     CDS             1..699
                     /gene="hdhA"
                     /locus_tag="OO433_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
     gene            928..1518
                     /gene="uidR"
                     /locus_tag="OO433_003206"
     CDS             928..1518
                     /gene="uidR"
                     /locus_tag="OO433_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6051574.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1906..3717
                     /gene="uidA"
                     /locus_tag="OO433_003207"
     CDS             1906..3717
                     /gene="uidA"
                     /locus_tag="OO433_003207"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6051575.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLEWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3714..5087
                     /gene="uidB"
                     /locus_tag="OO433_003208"
     CDS             3714..5087
                     /gene="uidB"
                     /locus_tag="OO433_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6051576.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYERYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARSGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5126..6391
                     /gene="uidC"
                     /locus_tag="OO433_003209"
     CDS             5126..6391
                     /gene="uidC"
                     /locus_tag="OO433_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6051577.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQRGLFDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPCENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(6617..8125)
                     /locus_tag="OO433_003210"
     CDS             complement(6617..8125)
                     /locus_tag="OO433_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6051578.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8226..9401)
                     /gene="manA"
                     /locus_tag="OO433_003211"
     CDS             complement(8226..9401)
                     /gene="manA"
                     /locus_tag="OO433_003211"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6051579.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9600..>10820
                     /gene="fumB"
                     /locus_tag="OO433_003212"
     CDS             9600..>10820
                     /gene="fumB"
                     /locus_tag="OO433_003212"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6051580.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNG"
BASE COUNT         2695 a         2528 c         2780 g         2817 t
ORIGIN      
        1 tggtaaagaa atcgccatta cattcgcgac agctggcgca tctgtggtgg tcagtgatat
       61 taacgccgac gcagctaacc atgttgtaga cgaaattcaa caactggggg ggcaggcatt
      121 tgcctgccgt tgtgatatta cttccgaaca ggaactctct gcactggcag atttgctatc
      181 agtaagctgg gtaaagttga tattctggtt aacaacgccg gtggcggtgg acctaaaccg
      241 tttgatatgc caatggcgga ttttcgctgt gcttatgaac tgaatgtgtt ttcttttttc
      301 catctgtcac aacttgttgc gccagaaatg gaaaaaaatg gcggtggcgt tattctgacc
      361 atcacttcta tggcggcaga aaataaaaat ataaacatga cttcctatgc atcatctaaa
      421 gctgcggcca gtcatctggt cagaaatatg gcatttgacc tgggtgaaaa aaatattcgg
      481 gtgaatggca ttgcgccggg ggcaatatta accgatgccc tgaaatccgt tattacacca
      541 gaaattgaac aaaaaatgtt acagcacacg ccgatcagac gtctgggtca accgcaagat
      601 attgctaacg cggcgctgtt cctttgctcg cctgcagcca gctgggtaag cggacaaatt
      661 ctcaccgtct ccggtggtgg ggtacaggag ctcaattaat acactaacga accggtaaac
      721 agccgtatgt gttgtttacc gggatagcgt gtgcaattca tcaacgtctc tgctaaacaa
      781 ctggcagcca aatcacggct attggttaac caatagctgg atgaaaagca tacgaataga
      841 gtgtgccttc gcactattca acagcaatga taggcgctca cctgacaacg cggtaaacta
      901 gttattcacg ctaactataa tggtttaatg atggataaca tgcagactga agcacaaccg
      961 acacggaccc ggatcctcaa tgctgccaga gagatttttt cagaaaatgg atttcacagt
     1021 gcctcgatga aagccatctg taaatcctgc gccattagtc ccgggacgct ttatcaccat
     1081 ttcatctcca aagaagcctt gattcaggcg attatcttac aggaccagga gagggcgctg
     1141 gcccgtttcc gggaaccgat tgaagggatt cattttgttg actatatggt cgagtctatt
     1201 gtctctctca cccatgaagc ctttgggcaa cgggcgctgg tggtcgaaat tatggcggaa
     1261 gggatgcgta acccacaggt cgccgccatg cttaaaaata agcatatgac gatcactgaa
     1321 tttgttgccc agcggatgcg tgatgcccag caaaaaggcg agataagccc agacatcaac
     1381 acgtcaatga cttcacgttt actgctggat ctgacctacg gtgtactggc cgatatcgaa
     1441 gcggaagatc tggcgcgtga agcgtcgttt gctcagggat tacgcgcgat gattggcggt
     1501 atcttaaccg catcctgatc ctctcatttc gcgggctggt gataactgtg cccgcgtttc
     1561 atactttaat ttccctgtac aaaatacaat ctagccggga tcggagaatt cccccccaaa
     1621 atattcaccg tagccatatg tcatgagagt ttatcgttct caagatgccc gaacgaacgt
     1681 tcgattgctt attttgtggc ttctgtaaac gctgttttaa agattaatgc gatctatatc
     1741 acgctgtgcg tattgcagat tttggttttt tgatcgcggt gtcagttctt tttatttcca
     1801 tttctcttcc atgggtttct cacagataac tgtgtgcaac acagaattgg ttaactaatc
     1861 agattaaagg ttgaccagta tttttatttg aatgaggagt cccttatgtt acgtcctgta
     1921 gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc
     1981 gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt
     2041 gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgtgggc
     2101 aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg
     2161 ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg
     2221 gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg
     2281 aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg
     2341 ggaatggtga ttaccgatga aaacggcaag aaaaagcagt cttacttcca tgatttcttt
     2401 aactatgccg ggatccatcg cagcgtaatg gtctacacca cgccgaacac ctgggtggac
     2461 gatatcaccg tggtgacgca tgttgcgcaa gactgtaacc acgcgtctgt tgactggcag
     2521 gtggtagcaa atggtgatgt cagcgttgaa ctgcgtgatg cggatcagca ggtggttgca
     2581 actggacaag gtaccagcgg gactttgcaa gtggtgaatc cccacctctg gcaaccgggt
     2641 gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac
     2701 ccgctgcgcg tcggcatccg gtcagtggca gtgaaggacg aacagttcct gattaaccac
     2761 aaaccgttct actttactgg ctttggtcgt catgaagatg cggatttgcg aggcaaagga
     2821 ttcgataacg tgctgatggt gcacgatcac gcattaatgg actggattgg ggccaactcc
     2881 taccgaacct cgcattaccc ttacgctgaa gagatgctcg agtgggcaga tgaacatggc
     2941 atcgtggtga ttgatgaaac tgcagctgtc ggctttaacc tctctttagg cattggtttc
     3001 gaagcgggca acaagccgaa agaactgtac agcgaagagg ctgtcaacgg ggaaactcag
     3061 caggcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg
     3121 gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg agaatatttc
     3181 gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat
     3241 gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg
     3301 aaccgttatt acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg
     3361 gaaaaagaac ttctagcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac
     3421 ggcgtggata cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat
     3481 cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt
     3541 gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt
     3601 aacaagaagg ggatcttcac ccgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa
     3661 aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaatgaatc
     3721 aacaactctc ctggcgcacc atcgtcggct acagcctcgg tgacgtcgcc aataacttcg
     3781 ccttcgcaat gggggcgctc ttcctgttga gttactacac cgacgtcgct ggcgtcggtg
     3841 ccgctgcggc gggcaccatg ctgttattgg tgcgggtatt cgatgccttc gccgacgtct
     3901 ttgccggacg agtggtggac agtgtgaata cccgctgggg aaaattccgc ccgtttttac
     3961 tcttcggtac tgcgccgtta atgatcttca gcgtgctggt attctgggta ccgaccgact
     4021 ggagccatag cagcaaagta gtgtatgcat atttgaccta catgggcctc gggctttgct
     4081 atagcctggt gaatattcct tatggttcac ttgctaccgc gatgacccaa caaccacaat
     4141 cccgcgcccg tctgggcgcg gctcgtggga ttgccgcttc attgaccttt gtctgcctgg
     4201 catttctgat agggccgagc attaagaact ccagcccgga agagatggtg tcggtatacc
     4261 atttctggac gattgtgctg gcgattgccg gaatggtgct ttacttcatc tgcttcaaat
     4321 cgacgcgtga gaatgtggta cgtatcgttg cgcagccgtc attgaagatc agtctgcaaa
     4381 ccctgaaacg gaatcgcccg ctgtttatgt tgtgcatcgg tgcgctgtgt gtgctgattt
     4441 cgacctttgc ggtcagcgcc tcgtcgttgt tctacgagcg ctatgtgtta aatgataccg
     4501 ggctgttcac tgtgctggta ctggtgcaaa acttggttgg tactgtggca tcggcaccgc
     4561 tggtgccggg gatggtcgcg aggagcggta aaaagaatac cttcctgatt ggcgctttgc
     4621 tgggaacctg cggttatctg ctattcttct gggtttccgt ctggtcactg ccggtggcgt
     4681 tggttgcgtt ggccatcgct tcaattggtc agggcgttac tatgactgtg atgtgggcac
     4741 tggaagctga taccgtagaa tacggtgaat acctgaccgg cgtgcgaatt gaagggctca
     4801 cctattcact attctcattt acccgtaaat gcggtcaggc aatcggaggt tcaattcctg
     4861 cctttatttt gggattaagc ggatatatcg ccaatcaggt gcaaacgccg gaagttatta
     4921 tgggcatccg cacatcaatt gccttagtac cttgcggatt tatgctactg gcattcgtta
     4981 ttatctggtt ttatccgctc acggataaaa aattcaaaga aatcgtggtt gaaattgata
     5041 atcgtaaaaa agtgcagcag caattaatca gcgatatcac taattaatat tcaataaaaa
     5101 taatcagaac atcaaaggtg caactatgag aaaaatagtg gccatggccg ttatttgcct
     5161 gacggctgcc tctggcctta cctctgctta tgcggcgcaa ctggctgacg atgaagcggg
     5221 actacgcatc agactgaaaa acgaattgcg cagggcggat aagcccagtg ctggcgcggg
     5281 aagagatatt tacgcatggg tacagagagg attgttcgat ttcaatagtg gttattattc
     5341 caatattatt ggcgttgaag gcggggcata ttatgtttat aaattaggtg ctcgtgctga
     5401 tatgagtacc cggtggtatc ttgatggtga taaaagtttt ggctttgccc tgggggcagt
     5461 aaaaataaaa ccctgtgaaa atagcctgct taaattaggt cgcttcggga cggattatag
     5521 ttatggtagc ttaccttatc gtattccgtt aatggctggc agttcgcaac gtacattacc
     5581 gacagtttct gaaggagcat taggttattg ggctttaaca ccaaatattg atctgtgggg
     5641 aatgtggcgt tcacgagtat ttttatggac tgattcaaca accggtattc gtgatgaagg
     5701 ggtgtataac agccagacgg gaaaatacga taaacatcgc gcacgttctt ttttagccgc
     5761 cagttggcat gatgatacca gtcgctattc tctgggggca tcggtacaga aagatgtttc
     5821 caatcagata caaagtattc tcgagaaaag cataccgctc gacccgaatt atacgttgaa
     5881 aggggaattg ctcggctttt acgcgcagct cgaaggttta agtcgtaata ccagccagcc
     5941 caatgaaacg gcgttggtta gtggacaatt gacctggaat gcgccgtggg gaagcgtatt
     6001 tggcagtggt ggttatttgc ggcatgcaat gaatggtgcc gtggtggata ccgacattgg
     6061 ctatcccttt tcattaagtc ttgatcgtaa ccgtgaagga atgcagtcct ggcaattggg
     6121 cgtcaactat cgtttaacgc cgcaatttac actgacattt gcaccgattg tgactcgcgg
     6181 ctatgaatcc agtaaacgag atgtgcggat tgaaggcacg ggtatcttag gtggtatgaa
     6241 ctatcgggtc agcgaagggc cgttacaagg gatgaatttc tttcttgctg ccgataaagg
     6301 gcgggaaaag cgcgatggca gtacgctggg cgatcgcctg aattactggg atgtgaaaat
     6361 gagtattcag tatgacttta tgctgaagta agaaataacg ccggagagaa aaacctccgg
     6421 cgtttcagat tgttgacaaa gtgcgcgttt tttatgccgg atgcggcgta aacgccttat
     6481 ccagcctaca aaaactcata aattcaaagt attgcaggaa aaggtaggcc tgataagcgt
     6541 agcgcatcag gcaatctctg gtttgttttc agatgaaaac gccgaagtga aaattctccg
     6601 gcgttttggc cgtgaattac tgctgcggaa ttgccggtac agccggaacg ttaagagctg
     6661 gcatcgcaaa catgccaaca aaatcttcta acgacatttt ctgcccattt aacgttatct
     6721 gaccgttagt atattgcagg ctggtggtga tggtattgtc ctgcaaggtg gtcagacgga
     6781 acatctgccc cattgctgat gcaccttcaa cttgctgttt cgccagtttt ttcgcttgat
     6841 cttcctgata accttccagc ttcgctacct gagtcataaa ctcagttgcc atatccaccg
     6901 gaatggtcag tttcgcatcc agagatttaa ccgaacgatc tacttcctgc gccagcgttt
     6961 gcggcgcttc tttagtcgtt gccggatctt tcaggaacag cgacagattc agcgcacttt
     7021 caccctgact gtttttccag cttagcggcg cgacagtaat caccggatcg cctttcaaca
     7081 tcagcggcag ggcgctaaag aaggcttccg tcactttctc ctgataaagt tcgggattgt
     7141 tggcaatttc tggctgtgcc agcagcgcct gagtttgcgc gttatattgc tggctgaact
     7201 gatgccaggc ttcaccatca atctggccga cttttaaagt cagcttgccg ctgcccagat
     7261 cctgattctg taccttcagg ctgtttagcg agtaatccag ttggctattg atcgttttac
     7321 cgtcattgac cagatccgat ttaccgctga tctccatgcc ttccagcagt gccagttctt
     7381 tgccttccac tgaaatggtc attttttcca gtgacagttt ttggtttcct acgcgctcac
     7441 caaaacttgc cagcgtgctg gaaccgtcgg ttttcagatt attaaaggtc aactgcactt
     7501 tctggttgta ttcgttaact gcgtctatcc gaccactttg cgcctccccg gaaagggaga
     7561 tggctttgcc gtctctgtca gcatttaact ggaactcgcc gccgctaaag gcgacttttt
     7621 catccttttg ctcgtaattc agtggcttga gcgaaatatc ggaactggaa tcaccgctgt
     7681 aaccaatgcg cgagttaatc tcaaaaggcg tttcaccttt tgccatatca aacagtggtt
     7741 tgcttacttc gttattaacc agcgtggttt gaattgatgc catcgacggg atcaggttca
     7801 gttttttaag ctgggcaagc gggaagggac catgatcaac cgattcgttg aagatgacgc
     7861 tctgaccgct tttaatccac ggattttctt tcccggcaat gggtttcacc aacagttgca
     7921 actggctgct gaatacgccg cgatgatagt tttgataact cacttccagg ttggattcag
     7981 gagctgtcag ttttagttgc gcgttcgcct gcgcgaccat gtcttcgaga tgggtttcaa
     8041 tcttcttgcc tgtataccat gcgccgcctg tccagactac gcctagcgca acaatgacgc
     8101 ctaccgctac cagcgattta ttcataatga ttatccataa aatgaaatca ggcggactgg
     8161 ccgcctgaag gtgttataag cctttaataa gcttagcaag agatgttaat tttttcagta
     8221 agctcttaca gcttgttgta aacacgcgct aaacggccgt ggcctttgac agtcaccggt
     8281 gattcgttgg cggcaataaa cgctgattca cccggtttaa gctgtaactg ctgagaacct
     8341 ttccacaacg ttgcatcgcc ttcgacgcag aacaaaatgg cggcactctg ctggctaatg
     8401 gtggtttctt tatcactaag gtcatgcagc gagaaggcaa aatcatccac tggaatcggg
     8461 aagtccagtt ctgcaccttg tttcaccggc tgggtcaaca actggttagc cggtttggct
     8521 tcgaatttca cattggcaac cagttccgga atatcaatgt acttaggcgt cagacccgca
     8581 cgcagcacgt tatcggagtt cgccatcact tccagcgcca cgccttgcag gtaagcgtgc
     8641 ggtgtttcag cgaacaggaa catcgcttcg ccaggattca atttcaccac attcagtaac
     8701 agcggggaga acaggccgct gtcttccggg taaaattcag aaattaaacg aatcgtttgc
     8761 cacggttcac cctgctgact gtcgagcgcc gattttaaaa tcgccagcgc gcgggatttt
     8821 tcttcaccct gcatattcaa caggctggcg aacagttcgc ttaaacgttc ggcattaggc
     8881 tgttgtaaaa agtgagcaat tgccggatgt gcacctgcga ctggctggag tagggagaca
     8941 atctcggaaa actcacgaaa cgcgttcatc gcaaggaaag gcgtcagcgc aaaaaccagc
     9001 tccggcttgt ggttaggatc tttatagtta cgctcggcgg catccatcgg gatacctgcg
     9061 gcattttctt tggcaaaacc gatttcagaa ttgcgtttgt ttggatgaac ctgaatggag
     9121 agcggctgtg ctgcgcataa tactttgaac aggaaaggca gttcgccaaa gcgtttggca
     9181 acggcctctc cgagcagagt cgatttatca ctctcaatca catcacgcag tgaaacgata
     9241 tctccggcgg cattctgcac tcgtgaactg cttttcggat gtgcgcccat ccacagctcg
     9301 gccatcggct ggctggacgg attttccata ccataaagtt cagtcaacgc cgttttgctg
     9361 ccccaggcat agttttgcac tgagttaatg agtttttgca tgatcaatcc ctgttttaat
     9421 gtggaaatta atcccactat taaagcaaga atcctacggg aagtaacctg gagccgcaaa
     9481 aagtcgtact agtctcagtt tttgttaaaa aagtgtgtag gatattgtta ctcgctttta
     9541 acagggcaac ggaacacccg cccagagcat aaccaaacca ggcagtaagt gagagaacaa
     9601 tgtcaaacaa accctttcat tatcaggctc cttttccact caaaaaagat gatactgagt
     9661 attacctgct aaccagcgaa cacgttagcg tatctgaatt tgaagggcag gagattttga
     9721 aagtcgcacc cgaagcgtta actctgttgg cgcgtcaggc gtttcatgat gcgtcattta
     9781 tgctgcgtcc ggctcaccaa caacaggtgg ccgacattct gcgtgacccg gaagccagcg
     9841 aaaatgataa atatgtggcg ctgcaattcc tgcgcaactc cgacatcgcg gcgaaaggtg
     9901 ttctgccaac ctgtcaggat accggcactg cgattattgt tggtaaaaaa gggcagcgtg
     9961 tatggaccgg tggctgtgat gaagcggcgc tggcgcgcgg tgtctataac acttatatcg
    10021 aagataattt gcgctactcg caaaacgcgc cgctggatat gtataaagag gtgaataccg
    10081 gcaccaatct gccagcgcag atcgatcttt atgccgttga tggcgacgag tacaaattcc
    10141 tctgtatcgc caaaggtggt ggttcggcaa acaagacgta tctctatcag gaaaccaaag
    10201 cgttactaac gccggggaaa ctgaaaaatt acctggttga gaagatgcgc acgctgggta
    10261 cggcggcctg tcctccgtat catattgcgt tcgtaattgg tggaacttct gcagaaacta
    10321 accttaaaac agtgaaactg gcttccgcta aatactatga cgaactgcca acggaaggga
    10381 atgagcacgg tcaggcattc cgcgatgtgg aactggaaaa agaattgctg atcgaagcgc
    10441 aaaatctcgg tctgggtgcg cagtttggtg gtaaatactt cgctcacgac atacgcgtga
    10501 ttcgcctgcc acgtcacggc gcatcctgtc cggttggtat gggtgtttcc tgctctgctg
    10561 accgtaatat caaagcgaag atcaaccgtc aggggatctg gatcgaaaaa ctggaacata
    10621 atccaggtaa atatatccct gaagagctgc gtaaagcggg agaaggcgaa gcggtgcgcg
    10681 ttgaccttaa ccgtccgatg aaagagatcc tcgcacagtt gtcgcagtat cccgtttcta
    10741 cacgcttatc gcttaacggc acgattatcg tcggtcgtga tattgctcac gccaaactga
    10801 aagagcggat ggataacggt
//
LOCUS       DAJTSL010000139        10736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTSL010000139 DAJTSL010000000
VERSION     DAJTSL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.139"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(76..273)
                     /locus_tag="OO433_003213"
     CDS             complement(76..273)
                     /locus_tag="OO433_003213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051581.1"
                     /translation="MLPSETMIWQPEFTDKTLSKKPGAVQLLDKIITIHRVKQVVTAS
                     LKLRDYDGQVAEAGDAANLLI"
     gene            complement(239..370)
                     /locus_tag="OO433_003214"
                     /pseudo
     CDS             complement(239..370)
                     /locus_tag="OO433_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            652..2010
                     /locus_tag="OO433_003215"
     CDS             652..2010
                     /locus_tag="OO433_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR6051582.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(2249..3634)
                     /locus_tag="OO433_003216"
     CDS             complement(2249..3634)
                     /locus_tag="OO433_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6051583.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSSREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3684..4031)
                     /gene="tnpB"
                     /locus_tag="OO433_003217"
     CDS             complement(3684..4031)
                     /gene="tnpB"
                     /locus_tag="OO433_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6051584.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(4028..4408)
                     /locus_tag="OO433_003218"
     CDS             complement(4028..4408)
                     /locus_tag="OO433_003218"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR6051585.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4763..5197)
                     /locus_tag="OO433_003219"
     CDS             complement(4763..5197)
                     /locus_tag="OO433_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR6051586.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(5185..5586)
                     /locus_tag="OO433_003220"
     CDS             complement(5185..5586)
                     /locus_tag="OO433_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051587.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5846..6415)
                     /locus_tag="OO433_003221"
     CDS             complement(5846..6415)
                     /locus_tag="OO433_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR6051588.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(7160..7303)
                     /locus_tag="OO433_003222"
                     /pseudo
     CDS             complement(7160..7303)
                     /locus_tag="OO433_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(7637..7903)
                     /locus_tag="OO433_003223"
     CDS             complement(7637..7903)
                     /locus_tag="OO433_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051589.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            8044..8250
                     /locus_tag="OO433_003224"
     CDS             8044..8250
                     /locus_tag="OO433_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6051590.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            8345..8947
                     /locus_tag="OO433_003225"
     CDS             8345..8947
                     /locus_tag="OO433_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051591.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            10147..>10736
                     /locus_tag="OO433_003226"
     CDS             10147..>10736
                     /locus_tag="OO433_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051592.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYL"
BASE COUNT         2566 a         2852 c         2703 g         2615 t
ORIGIN      
        1 ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa
       61 aaacaccatc atacactaaa tcagtaagtt ggcagcatca ccagcttctg ctacctgacc
      121 atcataatca cgcagcttca gtgaagcagt gaccacctgt ttaacccggt gtatcgttat
      181 gatcttatcc agcaattgaa ccgccccggg tttcttggag agtgttttat ctgtgaactc
      241 aggctgccag atcatcgttt ccgatggaag cataataagc tttttctgct tctgccggtg
      301 ggatatggcc ctgcctttcc agcaatcgtc gattgttata ccagtccacc cacgtgagtg
      361 tggtcagctc atcatactga cacgtgcatt acccctaccg cgataatgca accgtacaat
      421 atagggcgtt tcctaaacgc agccctcatc actccaatcc aacaagacac agcatttttg
      481 atcacgaaga aagacaaata atttccgtaa atgtttccgt tgttacaaat aacaacagtg
      541 ttttacattt ctttggcctc aggcccgcgc atgttgccat tcgatgtcat tttcctgact
      601 cgtttcttct ttatggtgta gtgtgatctt cactttatag atgctaacaa tatgaaaaga
      661 aaaattattc ccgtgctaat cggttgtacg ctctctttct ctgccctggc ggcacaacct
      721 acggctgagc gctatgtcgt cagttttcca gaaggcacgc acgtgaacta cgccggcgca
      781 tttgccagtg cattcccgaa cggccttccg gttgggatag gttctggcct gctgttcacc
      841 ggtaaacagg gcgatgccct gacattcgca accattaccg atcgcggtcc gaacgcggat
      901 tcaccgaaag aggggaaaaa cgaaactaaa attttcgtta cgccagattt cgcgccgctg
      961 ctgatgacga ttcgtgtaca aaacggcaaa gcggaggcca tcgatccccg tcctctgcat
     1021 gacgataaag gcgcgatcaa cggtctgccg ctggcaagcg acgtgattgg ctccaccaat
     1081 gaagtggcct tcagcgacac gttacataga ctgaaggggg ataatcgcgg gctggatacg
     1141 gaagggatca ctccggatgg taaaggaggt tactggctgt gcgatgagta tggcccgttc
     1201 ctgattaaca tcgacagtaa agggaaaatc ctggcaatcc acggtccgca ggcggcggaa
     1261 ggggaaaaag cgattgcggg cggtttgcca aatatcctca aatggcgtca ggcgaaccgc
     1321 ggctttgaag gcctcacccg tatgccggac gggcgtatta ttgtcgccgt gcaaagtacg
     1381 ctggatatcg acgctaagag caagaaaaag gcgctgttta cgcgtctggt gagcttcgat
     1441 ccggcaagcg gaaaaaccgc gatgtacggt tatcctatcg acagcgcggc ctacagcaaa
     1501 aacagcgacg ctaaaatcgg agacatcgtg gcgctcgata accagcacat cctgctgatc
     1561 gaacagggaa gagataaaaa caacagaatg cgtaacctga tttacgaggt cgatctcaat
     1621 aaggcgagcg acctgtcagg cttcgacaag ccgggtgagt atccggagtt tgatgatgag
     1681 aaaaccctgt cacagcgcgg cattaccctt gcgcagaaaa cgcaggttgt tgatctgcgc
     1741 tccttgggct ggcagcagga gaaggctgaa gggttggcgc tgatcgacag caaaacgctg
     1801 gcggtggcga acgataacga ctttggcgtg aaggtggcga tgcaacatcc ggtcgagggc
     1861 aagacattca aggattatcg ggtgaacgcg gaaggaaagc tgacgcttga cgacaagcag
     1921 gttgaaacga ccctcagagt gaagccactg gagaaaccgg agtctgacag tgaactgtgg
     1981 attgtgacgc tgccggaagc gttgaaataa gtttataagg tacggacagc agtatcgctt
     2041 agtttttcaa attcatgtat gtgaacaata ttggggttcc ggcgtagacc cccaatgaaa
     2101 gcgtcaccac gtgaaaacat gcctgcacgg tttacacgtc atcccatggt ggcagccggt
     2161 taactttcct gcgccaccag tggaaaaagg ccataagcag tccgaacaaa agtaagcgta
     2221 cagcgagggc cgtattgacg gggatgtgtt attcagctgg cagtgctatg cgccacggaa
     2281 gcagttcgct gacccggttg accggccagt ctgctatgac gccaagcaca tggcgaaggt
     2341 agctttctgg atccacgtca ttcagtttgc acgtcccgat caggctgtac agtagcgctc
     2401 cccgctcacc accatggcca gagccgaaga acagggtctt catcttttca cgcaaccagc
     2461 tttccaggga tttcaacagc ggtttcgttt ttcgctgacg ttcagcaagc cgctgctctg
     2521 ccggcattcc ccttatatcc gcctctatgg cgtacaactg accgatctgc tccagggctt
     2581 cttccgtcag tgctgacggg atgcggacgt gcacatcgtg gatctttcgg cgggcatgag
     2641 cccagcaggc agcttccgtt atcccaccat tgcgatacag ctcgttgaac ccggcgtacg
     2701 catccgcttg cagcacaccg ctgaagcaag caagatgagt ctgcggatgg atgctttttc
     2761 tgtccgggct gtaagcgaac cacactgcag gtgccaacgc tgacccggca ttgcggtcat
     2821 cacgaacata cgcccacaac cgcccggtct tcgtcttctt attacccggc agcagtacct
     2881 ggaccggggt atcatcggca tggagtttgc cgtcagtcat gacatagcca tgaagcgcct
     2941 cttccagcgg agacagcagc cggcagcatg catccaccca gcccgacagc agtgaacggc
     3001 tcagctccac accttgccgg ccgtatattt ctgactggca atacagcggg gtgtgctctg
     3061 catacttcga ggtcagcacg cgggccagca gccccggtcc ggcgataccc cgctcgatgg
     3121 gccgcgaagg tgcaggtgcc tgcacgatgg tatcgcactg agtacaggca tgtttttccc
     3181 gtaccgtccg gataacccgg aaggcgctac gcatcaactc cagctgttcg gcggtatcct
     3241 cgcccagata gctcagtgaa ccgccgcagt tcgggcagca cggcgccgca ggcaacagtc
     3301 gcttttcgtc acggggtagt gattcaggga acggcttacg ggtgcgggtc tgacgcaacg
     3361 gacgctgtac tgccgggtca tacaccctac cagtcagcgt atcgctctct ttctgaagcc
     3421 ggttcagatc ggcttccatt tgtgcgatac ggcgggagac tttttcggaa cgactgccga
     3481 agttcatccg gcggagttta tccagctgcg cctgcagatg gtctatttcg cgctcccggc
     3541 tgctcagctt ttcctgcagg gcgtggatca gcgcttcctg ttcggccagg cgctgtttca
     3601 gcaggaagat gtcgtcagaa gagatgtcgt tcataagccc gtattttacc gggcttattc
     3661 tgtgacaacc aggataaaga gatttacagc atggtcaggg aggtcagcag ccgcttgggc
     3721 tgtcgccagt cgataccttc cagcagcatc gccagctgcg cctgcgtaag gaacactttg
     3781 ccatcacggg ctgacggcca ggcgaagcgc ccacgctcca gccgtttggt caggaggcac
     3841 agtccgtcgc cggtggacca cagcagttta acctgactgc cgctgcggcc ccggaaaatg
     3901 aaaatatggc cggacatggg atcgtctttc agcgccgttt gtactttcgc agccaggccg
     3961 ttgaagctat ttctcatatc ggtgataccg gcaaccagcc aaattttggt cccggaaggt
     4021 aacgggatca tcgcttcagt tcctgtatca gcagagtcag gagcttttcg ctgacattgc
     4081 cattgaagcg gagcgtcccg tgccggaacg ttacctcaca gctgatactg agggtttccg
     4141 ggtcctctgc gagcgattct ggctgttcgg cagctgcatc gagagtcaca ggaagtagct
     4201 gggggctctc tgaagaaggt aatagcagct ttccctcgcg ccattgttgt cgccatttga
     4261 acaacagatt ggcgttaatg ccattttcaa gagcaagttt tgagatggat atcccgggtt
     4321 cacaggaggc agcaacgagc agctgtttaa attcgggagg ataattaggg cagccttttc
     4381 gcctgccggg agtcacattt ttctgcatat ctgacacttt ggttcccact acttatttgg
     4441 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
     4501 cactatttta tccctgttga atttggttaa gccggtgcag acgcgccggg aaacgcacac
     4561 ctgaaccggc acaataaaat acaatacaac cccctgttac tggagatacg aatggaatga
     4621 cgaggcagcc tgtcagtcac agggggctgt tggcataaaa tcaacggaaa ggccaccaac
     4681 accggaatta agctccacag tccccgcact ggcaatccat tcccgggaaa ggcatcaccg
     4741 caaactgctc cggaggcaac cattacagcc tctgtaacgg attatcagcc ctggcatctt
     4801 ccgacggcac ctcctgccgc ccccacgccc tgctgtatac cccggtaccg cagcgtgggc
     4861 agcacttttc cccctcataa tcttcgtcac acatcacaca aaaccacttt ctcaccttca
     4921 ccgtataatc cggaaccgtc atgccctgca gatgcaaacg acgggcctct ttctctgccg
     4981 ggtcaagcca ggtacatcgc agcaggattt tttcagcgac agtcatctgg tcatgttcaa
     5041 accacggtgg ttgtccgtca ttgccccgct gaagtgttaa cgggcatcca caggacacac
     5101 agcacaaaac gtcctgtcgg ttgtgcttcg cagcttcttt cgccgtaaca aaatggcctt
     5161 tgctgtcatt cgccatataa cagttcagaa ttcgcatatt tcctcctgag ttgtccgggg
     5221 aatactgaat cctccggtct ggcagtgtgt gcagtactgc tccccataat aatcgtggtg
     5281 acattgtctg cagtgccagc tggctttacg caccacgggt aaggcatccg gtacgaattg
     5341 ctgcagacgt ttaatcaact gtatttctct gcgctccggc ctgacataag ggcactgttg
     5401 accgtgctct gtcagcctgt cgtcagtgtg ttcaaaccag ggaagttcag tttcgtattg
     5461 cggatggtat ctgagtgcac tgccacacaa gtggcaggtg tagttagcat aaggtgcggc
     5521 ctgtgcagta cgggcgcccg tcagacgtcc gttgccatca agagcgataa aggattttgc
     5581 gtacatagtc atatacctca gccgccagac gacacgcagg caacagccgt cccggtacgg
     5641 gcagcgtggt cagggtgtga atggcggttc agttaaaatc tggactggag tttgggtaaa
     5701 gcaggctggt tgctgttaca gcactgtggt tgtcacacaa tggaacactg acgtaaaaaa
     5761 cagtcagtgg gcgagtggat acatcgttgc cgccagacaa caacccatac ttttggtttg
     5821 ccggatattg gcgcgccagt cgtgctcaac tccggctaca gccaaaattg cgctcaccgg
     5881 tgcagtgagc tttggtatgc tccttcgcca gatagtcagc acgttccagc acttgctgaa
     5941 agccggtgtc atcattacgc gccagccaca ccgccggcca ggccggaaaa tgcaccagcg
     6001 tggcatggca cccgacatcc actcccaggg cactgcacca cgcctgttta atcatcccgg
     6061 ccagagactc cggagcgcgg taatcaccgg cacgacacca ggtatcccgg ttgaccagca
     6121 gcatcaggtg atagtgtttt ttacctttgc actccccaaa ctcccgggcc cagacgtaat
     6181 gcagggttgt gggatgcacg cgtttacctt cccgatgctt acgtttctgg taagcgtcga
     6241 ttcgggcttt cagggcattg atgaagcggg atatcacagc tgcgtccgtt gctgccggta
     6301 catccgggag acgcaggtca acccgcaatg ccgtcaggcg gggatgaaca ttcagtgcgt
     6361 gccgcaccgt ctcacgaata cgttgctgcc agaaggggtt gtatttgtag gtcatggtta
     6421 aatctccgta tggttcatac ggaatagcca cgtcgtaaaa aatacgcaga gtccctgaag
     6481 tgacctccgg caggacactg tctcaggcac tctgtgataa cccgaacggt cgtctccgga
     6541 ctgacggttg aattttcagc gtcgtttttt aatgtaaaaa accggttacg ggtaaggctg
     6601 tgaggaggga gtgatggtga tacgcaaaaa gaagtgcagg gactgtggaa acgcgattac
     6661 tcataataca gtgtgttgcc cgtattgtgg ttctgtcgac cccttcggct actaccggaa
     6721 tacagaccgt atcgttacca tactgctggc gctgattatt gtggtcctgc tgactactgt
     6781 ctgcgtcagt gtgtgtatac tgtgtagctg gtgagataac ggttcatgta tcccctgagc
     6841 tgacagtgta ccgtgtatat taagcgtaac cgacaggttc tccttcatac catattaaat
     6901 tttccaggga ttgccgctgc cttatttcgt cccatacagg aaaactcatc tgggtttctg
     6961 acaaatacgc gaaactcaca gcccctcaat atcaaccaca cgttgtaagc cggtaatacc
     7021 tgaaaatcag cctattaccg cccacgcctc cggcaccatt cccttttgat ttcgaattct
     7081 ttgttgtcgc tataccgctt aatgttcccg gtcctccggt gcagataaca ccggagtcat
     7141 cgtagcgctg cttcaatatt caggtgacgt atttccagac ggaagccggc gcgcgatcgt
     7201 aaagtttgtt cattgtcagt tctgccagcc ggttgtcaac ggcagagtta aaggcttcca
     7261 gctccgcaag ggtaagttta taacgcgtgt gggaattcac ttttcccgcg tctcctgacg
     7321 tattacgggc tgccacttac ctggtgtccc gcaatacagc cttagtgttc cgttatcggg
     7381 gatccggacg caccgcaaca accggggaag tatttctggt ttagagcagt gtcaagaggg
     7441 atttctcagc ccctcactca ccggctcagt ctcagggcag gagcattaat acgctcagtt
     7501 catgtcttat acaggaatgg gcacaggggg agctggcgtg tcactgctga ttttacgcat
     7561 cagcgttcat gtatctgcac tctgatacat gaacgattca cagcgctctg ttaccggaga
     7621 gatttactct cttagcttac tctggcaaat cctttacgtt actctctgat gacttcttat
     7681 atttataata ccaatcagac agccttatcc catcggtgat atcagtctcc tcctcgccag
     7741 gaacgggctg actcactgta actacctccg aaaacaactc tgcaattatc agaacgcctg
     7801 cttctctccc tgtcctcacg aaaactatcc cctctttatc gcgcgtgcgt gcggaagcat
     7861 cttttcgcaa caaccacccg gtataccggt acggctcagc caccgcaacc tccccgttta
     7921 tctcctggcg gtcacattcc cgattatttt ttacgtttct ccccggttgt tatgccggtg
     7981 aaggtggcgc gctgttttca tgaccacacc ggctggttta acaacatccg gaggaacatt
     8041 ctcatggcta ccccagtttc actgatggat gaccagatgg tcgacatggc atttatcact
     8101 caactgaccg gcctgacaga taagtggttt tacaggctca tcagggatgg ggcctttccg
     8161 gctcccatca agctgggccg cagctcccgc tggctgaaaa gtgaagtgga agcctggcta
     8221 caggcgcgta ttgcacagcc ccgcccgtaa tttctgaccg ttatccgttc acccgcagca
     8281 gacgtaatcc ccggcctgcc ggcggcattc tgctgcctgt tatatccccg tgaggaatat
     8341 gaaaatgaaa caacagtatc agacccgcta cgaatggctc cacgaaagct accagaaatg
     8401 gctgaccggc ttcacccggc acgccgtatc ctggggcgtg tgtcatccga atatctacta
     8461 cttccataat ctgacgcccg gttgggtgtc attcaatggt gaaaaaccgg agattgccat
     8521 cgtaccccag agcctgcacc ggctgattta tggccctgac aaacgggcca cgccgcccct
     8581 ggatgatgat ctgatagtga atttatgcac cagtgaacat ctgctggttc atcatccgat
     8641 gctggaaggc attctcctgt ctgaatgtga acgtctgagg cagcgttctc tggcaaataa
     8701 actgatcagc ctcttccgtc agtttggtgg cacggagttg cgcctcaaac tggtctggct
     8761 ttgctggctt gatttaatga ccggaaacag ccttgaagac tggaaagaga acctgaaacg
     8821 gaaatcagaa aaagagctgg aggaatggat catcaaccgt cagaggcaga gcgcagcact
     8881 gacggatctg atggatcagt acgtgctcct ggcataccgc acaacggttg acgatagccg
     8941 caactgatgc cggttgcttc cggaaggtgt gacaaaatac ggaaatgatg atgctcccgg
     9001 cccccatgct cacctgcaga gcgtgttcgt cgtcatcata ctgagccatg acgggcgtct
     9061 taagggcatc ctgtcattgc tgagcaggca acaccaccag aggagccacg gacagggatg
     9121 ttctttacag cccgatacac gccaggttct cagcagactg aatgtgcagg tgcggttatt
     9181 caccagaaat ggcatgctgc ggtacatacc catcctggtg acaatatgta cgtggcgaca
     9241 gcgatcccgg cactgtggaa gagactcact cagcctgcag gatattcttt ccggcatccg
     9301 ggaatgtccg cctgaatgca aacaggatga tatttgaatc cgtaatactg ttctttcatc
     9361 ataaataatt tatgcagata cagttaaccg ttacagtagc cggtttgttt taccgggaaa
     9421 taatatccgt cagactgata ccggaacata tctgaaacac gttatgatgt tttgttatca
     9481 gaaatgttgt atattcttat ccgtggcgac agtttaatgc cctctggtat cacctgataa
     9541 tgtatatcct gcgatgattc gttatattta cgggacacgc ctcttttcat tgtgctcctt
     9601 ttcagacttc attttccgta agtgttcccc ggcataaagg aggtaccttt ctggcgttta
     9661 taactctctt tatcggtgac gccggtaata cagggaatac cacaccgaca aacgaggtat
     9721 taacagacga tatttcagtg tccataattt ttctgtccgg ttaaataacg tcagtctgct
     9781 gccctctgac tgtaatcatc aaagccggaa tgcataccgg gttacaacgt aaaaaaagtg
     9841 atgctggccc ccggtgcagc tggcgggcga aacatgttta cctttcaata ccccctgtct
     9901 gcagtctcca ctcttccatc ctcttaagct acgtcggcca catgagtgca cttccatgat
     9961 aacaggtatt cctgtcagca taacgaagcc ggttaccttc ccttcctcct gcatttcctg
    10021 tcagaatgac tatacagcgc gcagattgcc tttttattga ccatcttaat ataccgttat
    10081 aaacttcttt ttatcatatt attatcacct tattatgttt aatttttaag cagtcggaga
    10141 aagaggatgg ttcagaaaat tttgtcggat aaggttatga atgaaagaac aaatgcgtat
    10201 tactcttatt atcttggaga gcgtaatatc tccgtactgc cccttaatgt ttatgatccc
    10261 ccggaacgtt ttatcgctta cataaaaaaa aacagagaaa atctgaacat cacactttca
    10321 gattttgaac ttgaacaaat aatttctggt atgcgtctga aggccctggc cttcctggtc
    10381 cccctggaaa aaatatcctg gatcgcgggc agtgaacgcg catgcttatt ttcctggtat
    10441 ctgttgatgc agtttatcca gaataacagg gcaaaaataa gcgcagactt gcttcaaaag
    10501 aataaactgt atcttaaaga agagtatctg gaagggaatg ccttcccgtc ggactcatcg
    10561 acacagttca gacaaatact cagagttctc gatattcttt ctgataagaa tttgcgggat
    10621 gagtggataa tccagaccaa agacagatgg atgcgggcat ttaaatcaaa gagtcctttc
    10681 agttacctcc tcccggagaa cgaacatgaa tgcatatgga catggaatta tttgaa
//
LOCUS       DAJTSL010000140        10433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTSL010000140 DAJTSL010000000
VERSION     DAJTSL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.140"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(7..1905)
                     /gene="espL1"
                     /locus_tag="OO433_003227"
     CDS             complement(7..1905)
                     /gene="espL1"
                     /locus_tag="OO433_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCR6051593.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSELINQFARQLITLLD
                     TSDLSSMLSYCITQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAC
                     FLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQLVYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPVGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLITLFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLIYNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDETIDIFLNALTTPIAQELLNKKIVMDILAMKTRDGELGLFAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAHGTPVLYIAMSKGNEDVVLSYISTL
                     NIFAKKYSFSQRQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSLSFS
                     ADELKTYL"
     gene            2427..4355
                     /gene="thrS"
                     /locus_tag="OO433_003228"
     CDS             2427..4355
                     /gene="thrS"
                     /locus_tag="OO433_003228"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6051594.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHAARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4359..4901
                     /gene="infC"
                     /locus_tag="OO433_003229"
     CDS             4359..4901
                     /gene="infC"
                     /locus_tag="OO433_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6051595.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQRVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4998..5195
                     /gene="rpmI"
                     /locus_tag="OO433_003230"
     CDS             4998..5195
                     /gene="rpmI"
                     /locus_tag="OO433_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6051596.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5248..5604
                     /gene="rplT"
                     /locus_tag="OO433_003231"
     CDS             5248..5604
                     /gene="rplT"
                     /locus_tag="OO433_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6051597.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5721..5845
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5727..5771
                     /gene="pheM"
                     /locus_tag="OO433_003232"
     CDS             5727..5771
                     /gene="pheM"
                     /locus_tag="OO433_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6051598.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6054..7037
                     /gene="pheS"
                     /locus_tag="OO433_003233"
     CDS             6054..7037
                     /gene="pheS"
                     /locus_tag="OO433_003233"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6051599.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7052..9439
                     /gene="pheT"
                     /locus_tag="OO433_003234"
     CDS             7052..9439
                     /gene="pheT"
                     /locus_tag="OO433_003234"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6051600.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDCLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9444..9743
                     /gene="ihfA"
                     /locus_tag="OO433_003235"
     CDS             9444..9743
                     /gene="ihfA"
                     /locus_tag="OO433_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6051601.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(10047..10187)
                     /locus_tag="OO433_003236"
     CDS             complement(10047..10187)
                     /locus_tag="OO433_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCR6051602.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT         2741 a         2361 c         2662 g         2669 t
ORIGIN      
        1 agttggttat aaatacgtct ttagttcatc agcactaaaa ctcaggcttt ggctgattac
       61 atttatagca gcataatatg tttctacagt tttataatga ttatgatgaa tggctatatg
      121 aacagctgac atgttgtcat gatttttagc ggccaacagt gtgaataact gacgttgact
      181 aaaagaatat ttttttgcaa aaatattcag cgtcgatata taagataaca cgacgtcttc
      241 attacccttg ctcatggcga tgtataatac aggggttcca tgtgctgtag cgccttttaa
      301 taaatccatg atgttagctt tgctcaactt gtatttaaag gcgatgccat taattttaga
      361 gaggaaccgt gtgacacaca aagggtgatt attttccatt gcagcaaaca accctagttc
      421 accatcacgt gttttcattg ctaaaatatc catcactatt tttttgttta aaagctcttg
      481 tgctattgga gtggttaatg cattcagaaa gatatcgatt gtttcatcga acccatacaa
      541 catggagata tagagcccag gtgtttcgtt agcactttta gtttgcagta atttaaccag
      601 attatcttca tgaatgatat gattgtatat cagtgactga atttcttgca taaatatttt
      661 tacgttgtca gtatgtccgt tcatcatagc cagaaataaa gcaggtgtac caacgctatt
      721 ctgcgcagtt attatttcaa tgatttcttt ttcagggagt atgggcaact gtttgaataa
      781 tgtaataagc tgttgagtct ggcctgtggc cattattaac aacattgccg atggatgtat
      841 aggggcaaat ggcattcgtg atgcaatacc cgcgaaaata gcccctggaa ctcccacagg
      901 taataggggc agaatagcaa ttgcacagtt gcgaacatac ctatcagccc aggtctgagc
      961 atcgggacct gaactgatga acttatcaag cgaaagaaag ccccgtgctg tcctgtgggt
     1021 gcctctgacg gcaacgttgg tatcgttcgg gtcgtagacg gacactacat agtttaattg
     1081 tccatacact aactgcttta ttcgtagcct tactgacaaa gtatgattgt ctatatataa
     1141 aatcgcggac atatgactga cattgtttgc catcattcga caaaagaaaa tatctaaagc
     1201 gaagccaaaa tccttactcg cgataacgcg actccctgca ggaggatggc gaagtaatgc
     1261 tctgaactcc gcatcccgga ctaaggggac atgctgctgt atggattcaa cggaggagta
     1321 atgtttaatc aggttgactt tgccttttcc atttctcaat gaatcaaggg cgtattgcaa
     1381 tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc aaaaaacatg cactaccatt
     1441 gagatttact gttggttgta tagttttttc tctatggatt tttttagaca gtttcataca
     1501 atggttacga tcttcggcag ttaactgttc ctgctcaata taatccagta tgcttcgcaa
     1561 atggttagcc ttaataggcg tggtttccag aaataaggga atgatttctt ttttatagtg
     1621 taatgtaata agtatatttt ctgcatggat gggtgaagtg gcaaagttaa tgataaaaag
     1681 ggcagtggag taacaatttt gtgatatttt tcgacagttt gcggtaaatt cctgagtaat
     1741 acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga gttggcgcgc
     1801 aaattgatta ataagctctg atgaaggcgt tggtatgtcg ctatcgatgt gcaatctatg
     1861 aggagactta taatgagttc tgataatgat atcgttttga ctcataatct attcacctta
     1921 tatatactgg aggttttgag gatgatgatt aattattatc gccagaacga aatgtgcacg
     1981 aaaaattgct ataagaattg tgtcactttt atcattgtga tatgaccact tcgtcattaa
     2041 tctcatgatc gtttaaatac taccgccaaa tcacgcaacg aactttgctg gattcagttt
     2101 atgctgctgt aaatccgctc gcagtaaacc tttcagacgc accgtgatgt tatcagtggt
     2161 tctttaagcg ttttgctggt gtactcacta caaacgaatt gcgaatcgaa tcaatgtgaa
     2221 acggaaaggt acaatctccc tttttttagt gttaacatcg ttcaaccggg tggaaggtga
     2281 gaggttaatg tctttcattt gtgtacctga ttgcgaacca atttagcatt tgttggctag
     2341 atggtttcgc aatgaactgt taataaacaa atttttcttt gtatgtgatc tttcgtgtgg
     2401 gtcaccactg caaataagga tataaaatgc ctgttataac tcttcctgat ggcagccaac
     2461 gccattacga ccacgctgta agccccatgg atgttgcgct ggacattggt ccaggtctgg
     2521 cgaaagcctg tatcgcaggg cgcgttaatg gcgaactggt tgatgcttgc gatctgattg
     2581 aaaacgacgc acaactgtcg atcattaccg ccaaagacga agaaggtctg gagatcattc
     2641 gtcactcctg tgcgcacctg ttagggcatg cgattaaaca actttggccg cataccaaaa
     2701 tggcaatcgg cccggttatt gacaacggtt tttattacga cgttgatctt gaccgcacgt
     2761 taacccagga agatgtcgaa gcactcgaga agcggatgca tgagcttgct gagaaaaact
     2821 acgacgtcat taagaagaaa gtcagctggc acgcagcgcg tgaaactttc gccaaccgtg
     2881 gggagagcta caaagtctcc attcttgacg aaaacatcgc ccatgatgac aagccaggtc
     2941 tgtacttcca tgaagaatat gtcgatatgt gccgcggtcc gcacgtaccg aacatgcgtt
     3001 tctgccatca tttcaaacta atgaaaacgg caggggctta ctggcgtggc gacagcaaca
     3061 acaaaatgtt gcaacgtatt tacggtacgg cgtgggcaga caaaaaagca cttaacgctt
     3121 acctgcagcg cctggaagaa gccgcgaaac gcgaccaccg taaaatcggt aaacagctcg
     3181 acctgtacca tatgcaggaa gaagcgccgg gtatggtatt ctggcacaac gacggctgga
     3241 ccatcttccg tgaactggaa gtgtttgttc gttctaaact gaaagagtac cagtatcagg
     3301 aagttaaagg tccgttcatg atggaccgtg tcctgtggga aaaaaccggt cactgggaca
     3361 actacaaaga tgcaatgttc accacatctt ctgagaaccg tgaatactgc attaagccga
     3421 tgaactgccc gggtcacgta caaattttca accaggggct gaagtcttat cgcgatctgc
     3481 cgctgcgtat ggccgagttt ggtagctgcc accgtaacga gccgtcaggt tcgctgcatg
     3541 gcctgatgcg cgtgcgtgga tttacccaag atgacgcgca tatcttctgt actgaagaac
     3601 aaattcgcga tgaagttaac ggatgtatcc gtttagtcta tgatatgtac agcacttttg
     3661 gcttcgagaa gatcgtcgtc aaactctcca ctcgtcctga aaaacgtatt ggcagcgacg
     3721 aaatgtggga tcgtgctgag gcggacctgg cggttgcgct ggaagaaaac aacatcccgt
     3781 ttgaatatca actgggtgaa ggcgctttct acggtccgaa aattgaattt accctgtatg
     3841 actgcctcga tcgtgcatgg cagtgcggta cagtacagct ggacttctcc ttgccgtctc
     3901 gtctgagcgc ctcctatgtg ggcgaagaca acgagcgtaa ggtaccggta atgattcacc
     3961 gcgcaattct ggggtcgatg gaacgtttca tcggtatcct gaccgaagaa ttcgctggtt
     4021 tcttcccgac ctggcttgcg ccagttcagg ttgttatcat gaatattacc gattcacagt
     4081 ctgaatacgt taacgaattg acgcaaaaac tatcaaatgc gggcattcgt gttaaagcag
     4141 acttgagaaa tgagaagatt ggctttaaaa tccgcgagca cactttgcgt cgcgtcccat
     4201 atatgctggt ctgtggtgat aaagaggtgg aatcaggcaa agttgccgtt cgcacccgcc
     4261 gtggtaaaga cctgggaagc atggacgtaa atgaagtgat cgagaagttg caacaagaga
     4321 ttcgcagccg cagtcttaaa caattggagg aataaggtat taaaggcgga aaacgagttc
     4381 aaacggcgcg ccctaaccgt atcaatggcg aaattcgcgc ccaggaagtt cgcttaacag
     4441 gtctggaagg cgagcagctt ggtattgtga gtctgagaga agctctggag aaagcagaag
     4501 aagccggagt agacttagtc gagatcagcc ctaacgccga gccgccggtt tgtcgtataa
     4561 tggattacgg caaattcctc tatgaaaaga gcaagtcttc taaggaacag aagaaaaagc
     4621 aaagagttat ccaggttaag gaaattaaat tccgtcctgg tacagatgaa ggcgactatc
     4681 aggtaaaact ccgcagcctg attcgctttc tcgaagaggg tgataaagcc aaaatcacgc
     4741 tgcgtttccg cggtcgtgag atggcgcacc agcaaatcgg tatggaagtg cttaatcgcg
     4801 tgaaagacga tttgcaagaa ctggcagtgg tcgaatcctt cccaacgaag atcgaaggcc
     4861 gccagatgat catggtgctc gctcctaaga agaaacagta aggccttcaa gtagcactgt
     4921 ctgtggagcc ttcgggcttc acaggctttg ttcgcctttg tttcgtttat taacaatgcg
     4981 aagtggaagt tattaaaatg ccaaaaatta agaccgtacg cggtgctgct aagcgcttca
     5041 aaaaaaccgg taaaggtggt tttaagcaca agcacgctaa cctgcgtcac attctgacca
     5101 aaaaagcgac caaacgtaaa cgtcacctgc gtccgaaagc catggtttcc aaaggcgatc
     5161 tgggcctggt aatcgcgtgc ctgccgtacg cataagccgt taacgttttt aactttttaa
     5221 ttagaatata gatacaggag agcacatatg gctcgcgtaa aacgtggtgt tattgcacgc
     5281 gcacgtcaca agaaaatttt gaaacaagct aaaggctact acggtgcgcg ttctcgcgta
     5341 taccgcgttg ccttccaggc tgttatcaaa gctggtcagt atgcttaccg tgaccgtcgt
     5401 caacgtaagc gtcagttccg tcaactgtgg attgcacgta tcaacgcagc agcacgtcag
     5461 aacggtattt cttacagcaa attcatcaat ggcctgaaaa aagcctctgt tgaaatcgac
     5521 cgtaagatcc tggctgatat cgcagtattc gacaaagtag cgttcaccgc tctggttgaa
     5581 aaagcgaaag cagctctggc ataagccagt tgaaagaggg agcttgtctc cctcttttcg
     5641 tttcaacgcc atcaaaacat tgacttttat cgccgtagcc ttttcaataa aggtcttttg
     5701 aagagtacca aaaggtaacg caagcaatga atgctgctat tttccgcttc tttttttact
     5761 ttagcacctg aatccaggag gctagcgcgt gagaagagaa acggaaaaca gcgcctgaaa
     5821 gcctcccagt ggaggctttt tttgtatgcg cgttttgaaa aataaaaagc cagtctatca
     5881 ggcgtaattg tcgcagccag tctggatacg gccagcgcgg agaaaccgga gcgtatttaa
     5941 gtacgtgaga atttcgagca ctgcccggga ccaaaatggc aagtaaaata gcctgatggg
     6001 ataggctcta agtccaacga accagtgtca ccactgacac aatgaggaaa accatgtcac
     6061 atctcgcaga actggttgcc agtgcgaagg cggccattag ccaggcgtca gatgttgccg
     6121 cgttagataa tgtgcgcgtc gaatatttgg gtaaaaaagg gcacttaacc cttcagatga
     6181 cgaccctgcg tgagctgccg ccagaagagc gtccggcagc tggtgcggtt atcaacgaag
     6241 cgaaagagca ggttcagcag gcgctgaatg cgcgtaaagc ggaactggaa agcgcagcgc
     6301 tgaatgcgcg tctggcggcg gaaacgattg atgtctccct gccaggtcgt cgcattgaaa
     6361 acggcggtct gcatccggtt acccgtacca tcgaccgtat cgaaagtttc ttcggtgagc
     6421 ttggctttac cgtggcaacc gggccggaaa tcgaagacga ttatcataac ttcgatgctc
     6481 tgaacattcc tggtcaccac ccggcgcgcg ctgaccacga cactttctgg tttgacacta
     6541 cccgcctgct gcgtacccag acctctggcg tacagatccg caccatgaaa gcccagcagc
     6601 caccgattcg tatcatcgcg cctggccgcg tttatcgtaa cgactacgac cagactcaca
     6661 cgccgatgtt ccatcagatg gaaggtctga ttgttgatac caacatcagc tttaccaacc
     6721 tgaaaggcac tctgcacgat ttcctgcgta acttctttga ggaagatttg cagattcgct
     6781 tccgtccttc ctacttcccg ttcaccgagc cttctgcaga agttgacgtc atgggtaaaa
     6841 acggtaaatg gctggaagtg ctgggttgcg ggatggtgca tccgaacgtg ctgcgtaacg
     6901 ttggcatcga cccggaagtt tactctggtt tcgccttcgg gatggggatg gagcgtctga
     6961 ctatgttgcg ttacggcgtc accgacctgc gttcattctt cgaaaacgat ctgcgtttcc
     7021 tcaaacagtt taaataaggc aggaatagat tatgaaattc agtgaactgt ggttacgcga
     7081 atgggtgaac ccggcgattg atagcgatgc gctggcgaat caaatcacta tggcgggcct
     7141 ggaagttgac ggtgtagaac cggttgccgg tagcttccac ggcgtggtcg ttggtgaagt
     7201 ggttgagtgt gcgcagcatc cgaacgctga caaactgcgt gtgacaaaag tgaatgtcgg
     7261 cggcgatcgc ctgctggaca tcgtctgtgg tgcgccaaac tgccgtcagg gcctgcgtgt
     7321 ggcggtagct accattggtg ctgttctgcc gggtgatttc aaaattaaag cggcgaaact
     7381 gcgtggcgaa ccgtctgaag ggatgctgtg ctccttctct gaactgggaa tttctgacga
     7441 tcacagcggc attatcgaac tgcctgcaga tgcgccgatt ggcactgaca tccgcgaata
     7501 cctgaaactc gatgacaaca ccatcgaaat cagcgtaacg ccaaaccgtg ccgactgctt
     7561 aggtatcatt ggtgttgcgc gtgacgttgc cgtgctgaat cagctgccgc tggttgaacc
     7621 ggaaatcgtt ccggttggtg cgaccatcga cgacacgttg ccgattacag tcgaagcgcc
     7681 tgaagcctgc ccgcgctatc ttggccgtgt ggtaaaaggc attaacgtta aagcgccaac
     7741 tccgctgtgg atgaaagaaa aactgcgtcg ttgcgggatc cgttctatcg atgcagttgt
     7801 tgacgtcacc aactatgtac tgctcgaact gggccagccg atgcacgctt tcgataaaga
     7861 tcgcattgaa ggcggcattg tggtgcggat ggcaaaagag ggcgaaacgc tggtgctgct
     7921 tgatggcact gaagcgaagc tgaatgctga cactctggtc atcgccgacc acaacaaggc
     7981 gctggcgatg ggcggcatct ttggtggcga acactctggc gtgaatgacg aaacgcaaaa
     8041 cgtcttgctg gaatgcgctt tcttcagccc gctgtctatc accggtcgtg ctcgtcgtca
     8101 tggcctgcat actgatgcgt ctcaccgtta tgagcgtggc gttgatccgg cactgcagca
     8161 caaagcgatg gaacgtgcga cccgtctgct gatcgacatc tgcggtggtg aggctggtcc
     8221 ggtaattgat atcaccaacg aagcaacgct gccgaagcgt gcaaccatca ctttacgtcg
     8281 tagcaaactg gattgcctga tcggccatca tattgctgat gaacaggtaa ctgacattct
     8341 gcgtcgtctc ggctgcgaag tgaccgaagg caaagacgag tggcaggcag ttgcgccgag
     8401 ctggcgtttc gatatggaga ttgaagaaga tctggtcgaa gaagtcgcgc gtgtttacgg
     8461 ctacaacaac atcccggatg agccggtaca ggcaagcctg attatgggta ctcaccgtga
     8521 agctgacctg tcgctcaagc gcgtgaaaac gctgctcaac gacaaaggct atcaggaagt
     8581 gatcacctac agcttcgttg atccgaaagt gcagcagatg atccatccag gcgttgaagc
     8641 cttactgctg ccaagcccga tctctgttga aatgtcagca atgcgtcttt ctctgtggac
     8701 cggcctgctg gcaaccgtgg tgtacaacca gaaccgtcag cagaaccgtg tgcgcatttt
     8761 cgaaagcggt ctgcgtttcg taccagatac tcaggcaccg ttgggcattc gtcaggatct
     8821 gatgttagcc ggtgtgattt gcggtaaccg ttacgaagag cactggaacc tggcaaaaga
     8881 gaccgttgat ttctatgatt tgaaaggcga tcttgaatcc gttctcgacc tgaccggtaa
     8941 actgaatgag gttgagttcc gtgcagaagc gaatccggca ctgcatccgg ggcaatccgc
     9001 agcgatttat ctgaaagggg aacgtattgg ttttgttgga gttgttcatc ctgaactgga
     9061 acgtaaactg gatcttaacg gtcgcactct ggtgttcgaa ctggagtgga acaagctcgc
     9121 agaccgcgtg gtgcctcagg cgcgcgagat ttctcgcttc ccggcgaacc gtcgtgacat
     9181 cgcggtggtg gtcgcagaaa acgttcccgc agcggatatt ttatccgaat gtaagaaagt
     9241 tggcgtaaat caggtagttg gcgtaaactt atttgacgtg taccgcggta agggtgttgc
     9301 ggaggggtat aagagcctcg ccataagcct gatcctgcaa gataccagcc gtacactcga
     9361 agaagaggag attgccgcta ccgtcgccaa atgtgtagag gcattaaaag agcgattcca
     9421 ggcatcattg agggattgaa cctatggcgc ttacaaaagc tgaaatgtca gaatatctgt
     9481 ttgataagct tgggcttagc aagcgggatg ccaaagaact ggttgaactg tttttcgaag
     9541 agatccgtcg cgctctggaa aacggcgaac aggtgaaact ctctggtttt ggtaacttcg
     9601 atctgcgtga taagaatcaa cgcccgggac gtaacccgaa aacgggcgag gatattccca
     9661 ttacagcacg gcgcgtggtg accttcagac ccgggcagaa gttaaaaagc cgggtcgaaa
     9721 acgcttcgcc caaagacgag taatcagatc taactaaaaa ggccgctctg cggccttttt
     9781 tctttcaata actaattgat ttataatgtt ttatatctct tcgttccacg atgcgtccac
     9841 atttagtggg gggaggggag taagcccgct acattgtggg ctttgtctta tagtctgatc
     9901 actgtttcaa gtcggtgagg acaccaaaaa gaacccccgc agtttttacg ctgcgggttt
     9961 gttgttcatg tctgtgagat agggtgcctt atcgacctta ccctggcaac cgattgacgg
    10021 gggattgctc ccccgtcgcg gtttccttac tgcttacact gtaagaacgc cgcaaactcc
    10081 gctccccaga agctcatccg tatttcgcac agcgaaccgt gcagcatcca gatgatgagg
    10141 attgccgtca cgcagaacgt gatggccgta agcgattttt gcgacatagc gcttgctcct
    10201 ttttcgggga ggcgctaacc tatcacttgc ttagggtaga cggttagggc ctcggttaaa
    10261 caaaaatgtt ttccggggcc tttccacatc tggccttcag gtattccctc cgaccatcag
    10321 ccgaaaggca cccgcgcgta atctatcgct tttttgttac tccggcaatt ctgctcgtta
    10381 attctgaggt aagggcaaac tcatctgatt gtttcctctg tgtgaagttg gca
//
LOCUS       DAJTSL010000141        10332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTSL010000141 DAJTSL010000000
VERSION     DAJTSL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.141"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            121..987
                     /gene="folD"
                     /locus_tag="OO433_003237"
     CDS             121..987
                     /gene="folD"
                     /locus_tag="OO433_003237"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6051603.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQACIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            989..1201
                     /gene="ybcJ"
                     /locus_tag="OO433_003238"
     CDS             989..1201
                     /gene="ybcJ"
                     /locus_tag="OO433_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6051604.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1309..1830
                     /locus_tag="OO433_003239"
     CDS             1309..1830
                     /locus_tag="OO433_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6051605.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMEMLWWRRR"
     gene            complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OO433_003240"
     CDS             complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OO433_003240"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6051606.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQTRAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3425..3919
                     /gene="ppiB"
                     /locus_tag="OO433_003241"
     CDS             3425..3919
                     /gene="ppiB"
                     /locus_tag="OO433_003241"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6051607.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3922..4644
                     /gene="lpxH"
                     /locus_tag="OO433_003242"
     CDS             3922..4644
                     /gene="lpxH"
                     /locus_tag="OO433_003242"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6051608.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4762..5271
                     /gene="purE"
                     /locus_tag="OO433_003243"
     CDS             4762..5271
                     /gene="purE"
                     /locus_tag="OO433_003243"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6051609.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5268..6335
                     /gene="purK"
                     /locus_tag="OO433_003244"
     CDS             5268..6335
                     /gene="purK"
                     /locus_tag="OO433_003244"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6051610.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETVLTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YMGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6472..7363)
                     /locus_tag="OO433_003245"
                     /pseudo
     CDS             complement(6472..7363)
                     /locus_tag="OO433_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(7360..8175)
                     /locus_tag="OO433_003246"
     CDS             complement(7360..8175)
                     /locus_tag="OO433_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR6051611.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8186..9445)
                     /locus_tag="OO433_003247"
     CDS             complement(8186..9445)
                     /locus_tag="OO433_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR6051612.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGGCLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     IDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9455..10332)
                     /gene="fdrA"
                     /locus_tag="OO433_003248"
                     /pseudo
     CDS             complement(9455..10332)
                     /gene="fdrA"
                     /locus_tag="OO433_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT         2274 a         2934 c         2774 g         2350 t
ORIGIN      
        1 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
       61 gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg
      121 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
      181 caaaaagttc aggcgtgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
      241 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
      301 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgttg
      361 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
      421 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
      481 gacggtttcc atccttataa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
      541 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctt
      601 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgtc caatgagcat ggaactgttg
      661 ctcgcaggtt gcaccactac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta
      721 gaaaatgccg atctgttgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg
      781 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
      841 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccg
      901 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgcttca ggcgtgcgtt
      961 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     1021 cgttgagctg tgcgatttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     1081 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     1141 caaaatcgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     1201 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     1261 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     1321 attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct
     1381 ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg
     1441 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     1501 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct
     1561 gggcttattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     1621 tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt
     1681 ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg
     1741 tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt
     1801 atggaaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag
     1861 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     1921 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     1981 agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg
     2041 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     2101 aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca
     2161 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct
     2221 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg
     2281 cgcagcgcag tgtagagacg ctccagcgcc gcacgcgtct gcttcaggtt ctcttcgctg
     2341 tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg
     2401 tagtatttca gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg
     2461 cggtcaacca tcaccattcc ggagtgcatc cagtagttca catactgacc atcatgggca
     2521 caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg
     2581 tggatatcaa agtggttacc cagctgcttg cagttcatgg ccgaacattc aatgtgccag
     2641 ccaggacgac ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     2701 cacagaacga agtccatcgg gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc
     2761 tgcagctggt cgagatcctg acgcgacagc acgccataag tcggatcggt cgggacgtcg
     2821 aacatcacgt cgccgttgtc agccacataa gcgtgacctt tggcgatcag ttgttcagtg
     2881 agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     2941 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     3001 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc
     3061 acatacttca gcttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca
     3121 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg
     3181 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     3241 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     3301 catatttccc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat
     3361 aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg aacaggatgc
     3421 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgatgataa
     3481 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     3541 cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat
     3601 gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggtc tgaaaaatac
     3661 ccgtggtacg ctggcaatgg cccgtactca ggctccgcac tctgcaactg cacagttctt
     3721 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     3781 ctactgcgtg tttgctgaag tggttgaagg catggacgtg gtagacaaaa tcaaaggtgt
     3841 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     3901 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg
     3961 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc tcgcaaggcc
     4021 gacgcgctgt atattcttgg cgatctgttt gaagcgtgga ttggcgacga cgatcccaat
     4081 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     4141 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagtggc
     4201 atgacgttat tgccggaaga aaaggtgctc gaactttatg gccgccgggt gttgattatg
     4261 catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac
     4321 aaaccctggc tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc
     4381 gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta aatcgctggc gatcatggac
     4441 gttaaccaaa acgcagtggt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac
     4501 gggcataccc atcgcccggc ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc
     4561 gtggtactgg gtgcctggca tacggaaggt tcgatggtga aagtcacggc ggatgacgtt
     4621 gagctgattc attttccgtt ttaaaaaaac cacaactttg ctgattttac agccacgcaa
     4681 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     4741 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
     4801 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
     4861 aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
     4921 ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggtggcgca
     4981 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta
     5041 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
     5101 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca
     5161 gcacaaattc tcgcgactca cgataaagag ctacaccagc gtctgaatga ctggcgcaaa
     5221 gcccagaccg acgaagtgct ggaaaatccg gacccgcgag gtgcggcatg aaacaggttt
     5281 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca
     5341 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg
     5401 tgattaccgc tgagatcgaa cgctggccgg aaaccgtatt aacccgcgag ctggcgcgtc
     5461 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaagc
     5521 agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg
     5581 agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
     5641 gctatgacgg tcgcggtcaa tggcgtttac gcgccaatga aaccgaacag ttaccggcag
     5701 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
     5761 ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
     5821 aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc
     5881 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatatgggc gtgatggcga
     5941 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata
     6001 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
     6061 cgattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc
     6121 tgattggtag cgatgcgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt
     6181 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
     6241 catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca
     6301 gcggcgtgat ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat
     6361 gcggcgtaaa cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg
     6421 cgtcaagcgt cgaatcaggc atggtgcacc gattgccgga tgctctcatc actacagcga
     6481 aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca
     6541 cgcgggttta ctacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga
     6601 accatcggct ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcacgttg
     6661 ctgcggcgtt ccccagtttt catataccgc atcagcatcg gtgaggatca ccagtccatc
     6721 ggcattaatc tgctcggcga gcaacgcagt ggcgagatct ttatcaatca ccgcttcact
     6781 ccctgcccca tcttccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc
     6841 ctctttgagc aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc
     6901 caccacccgg cgcaaatatt taccatcacg tttcatctgc cagccgtaag ccgcttccag
     6961 tgcctcttgt tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc
     7021 cggatcatca ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg gtatctgcgg
     7081 ctgtgcgctc aagctctgcg ccagcatata gcctcatccc ctggctttcc gccaccagca
     7141 catccagcgg atacggttct acctctttcc acgccagatt ctgtaacgcc agcagcccca
     7201 cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagg cgtgccagcg
     7261 cgggtacagc actggcgata ttgcgatatt gattttccgc cgtcagcgcc tcaccgcgct
     7321 ggagtaaggc gttgccccca agagctacaa ccagtgtttt catggttttc cttgtaataa
     7381 ttgttggcca agccagaacc ccagcagcgt atctgcccct gacgtatgcc ccagcgccag
     7441 cagcgaatca atcgcaacag cggtacgttt cgggcaactc agagcatgga caaagtgcag
     7501 gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt
     7561 ggtaacgagt tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc gcgcatctaa
     7621 agcgccataa taccaagccg ccagcagcat tccgctcaac gtgtcatcat gacttggcgt
     7681 taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc
     7741 ctgaaaacag tgacgaaact ggcgtaattc agcggtcagc ggatcgcttg ccgccatcgc
     7801 cagtggtccg aaaagtccgg tttcctccgc gcgttgcatc catgcagctg caagcggttg
     7861 aggatgcgaa ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa
     7921 acgcccgagg cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc
     7981 gcataacgca tcgaattgcg cgcggcgaag cacccatcct ccggggccga aaccactacc
     8041 ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc aggttaatcg cccgccgcca
     8101 cactcccgct aaccgccagg actggcgata attcggtgcg ctactgctgg caagcagagg
     8161 atggatgatc gtcattgcgc gttcctcaac caatccccat gctttcagcc agtgcttcca
     8221 gcgcctgttc aaagcacgcc agcggtgccc gcacggtgcc tgcgccaatc tgcccgatcc
     8281 ccgcctcttt atgggcgata ccggtattga tgagtggcgt aatgccggtt tctaccacgc
     8341 gacgaatatc cagccccagg cacgcgccct gaaaatccca gcctgggatc tgcaactgca
     8401 tattgcgttc aaggtaaatt tccgccatct cttcagatac cgccctggcc gcttccatgc
     8461 cacccgcacc gacaaagcgc gttacgccag gcgctgcgat cattgccgca cctccgatac
     8521 caaaggtttc ggtaatcgcg ctatcgccca tatccgggtt cgcctgctcc tgcgagaagc
     8581 cggtgaaaaa cagaccttgc ggagtgttta caggcgcagt aaaccagcgt tcacccagcc
     8641 cgcttacccg aatcccgaac atattgccgt tgcgggtcat agccgtaacg atgctgcctg
     8701 cgcggatcat cgcgccagca tccatcgccg ccttgcagta ggccatcgcg aggttgagga
     8761 agaactgatc ggtcacgctg aggaaatcga tcacttccgc aatgtgctgt ttatcatgat
     8821 cgaggcgagc aatttgtggg gccagcgtac gcattaacag tgctgaggaa gcaatattgc
     8881 gttgatggaa ctcatcgccc atcgtaatgc cctgcgccat catcgccgtg agatcgatac
     8941 cgcgctccat gcgccccagc gccgcgctta atactggcat taacacatcg cgcatccagc
     9001 gatggcgagt caggacatct tcgccgtaag cgccaaaacg catcactttg ccgatacctt
     9061 cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc agcatcggca
     9121 tactggcaga agtaatgccg cccattggcc cgacggcatt cacatggtga caaggaatga
     9181 agttcacttc cccctgctcc agcatcgcca gtgcctgcgc ttcatctttc gcccagcctt
     9241 cgaacagaca tccgcccacg cacgcccctt tcatgggtcc ggtcatctcc tgccagcgca
     9301 ttggcggccc ggcgtgaagc agtgttttgc cctcgtttag ttcgctgata agtgaaaaag
     9361 ccggttgcac atccagccag tgtggacgag cacgacgaat ttgttcgata accgcagcat
     9421 tggcttgcgc cactgatgta aacatgggaa ccccttattg caaacgttct aataaacgag
     9481 ccagtttttt attgccgccg gcgactggcg accattggta atgcacaacc ggtttgctgg
     9541 cgctttgtag ctccagcgca aagctgcgta atccgatgtt aatcacggcg acgttttcca
     9601 gtaatgacgg tgtatgttgc tgtgtggcag gcgagagcgg ataaattaac gctgccgcca
     9661 gcaaggtggc ttccggcagc gaactcacta ccgcgatccc cgcatcttcc agcgtggcga
     9721 tttgctgcga gcggcattgc gggtcacgtt cagtgcctgt caccgtggca atggcataca
     9781 gtggttgatt atctgaacgc gcggcacagg ctttttgcta ggcgcttacc agcgaggcgg
     9841 caggatcggc agtcgcaccg aagccaatca cgacatcaag tagcaatacg cgcacttgtg
     9901 gtttagcgcc gagatcggca attaactggt tgcgtaaggc tgggtcgatc atcggatggg
     9961 gacgcccgac ggtgtagaaa tcatcgccga ggtcaataat ctggtgacca tcggcgtcga
    10021 gcatcatgcc gtgatgatgg gtatcgtcgg cttccacgcc aaggtgtcca gcaagtaatc
    10081 ccgccgcttc ggcagccagc gtaccgccgg tatacaaacc gcaaataaat ccgctgctga
    10141 caggcgctat tgcgttacat cgcgccgtga cgcgtgaaag caggcaagcc aggcgtgcgg
    10201 cctcatccag cgaggaggca aaccagacat tctcgtcgcg ggccaccgcc ggggtataac
    10261 ctaaaaacag cgccaccatc ggtttgccgg ttgctttcat ggcattaaca attttcagac
    10321 gcacagcttc gg
//
LOCUS       DAJTSL010000142        10276 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTSL010000142 DAJTSL010000000
VERSION     DAJTSL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.142"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            311..1609
                     /gene="kgtP"
                     /locus_tag="OO433_003249"
     CDS             311..1609
                     /gene="kgtP"
                     /locus_tag="OO433_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6051613.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1606..1929)
                     /locus_tag="OO433_003250"
     CDS             complement(1606..1929)
                     /locus_tag="OO433_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6051614.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OO433_003251"
     CDS             complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OO433_003251"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6051615.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYKAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRLFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OO433_003252"
     CDS             complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OO433_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6051616.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLHGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVGHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OO433_003253"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OO433_003253"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6051617.1"
                     /translation="MTENTVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREALAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OO433_003254"
     CDS             complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OO433_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6051618.1"
                     /translation="MNTVCTHCQSINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7529..8566
                     /locus_tag="OO433_003255"
     CDS             7529..8566
                     /locus_tag="OO433_003255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6051619.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAELVQSITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OO433_003256"
     CDS             complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OO433_003256"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6051620.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9608..9991
                     /gene="grcA"
                     /locus_tag="OO433_003257"
     CDS             9608..9991
                     /gene="grcA"
                     /locus_tag="OO433_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6051621.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10047..>10276)
                     /locus_tag="OO433_003258"
     CDS             complement(10047..>10276)
                     /locus_tag="OO433_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCR6051622.1"
                     /translation="ILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCWALAG
                     HLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2551 a         2604 c         2648 g         2473 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgcc tgatgcgacg
       61 ctgcgcgtct tatactccca catatgccag attcagcaac ggatgcggtt tccccaactt
      121 gcctacttcc atacgtgtcc tccttaccag aatttatcct taagctcctc aataaccatt
      181 ttcctgctaa ctaaattcat ggttaatgtt gcataataat acgtaacaaa tgtataacat
      241 tttctttacc aaaagaaata aacaaaagtg accaacaaaa gcatcggatt acggcaggag
      301 acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac
      361 tcgtcgccgc atttgggcga ttgtgggggc ctcttcaggt aatctggtcg agtggttcga
      421 tttctatgtc tactcgttct gttcactcta cttcgcccac attttcttcc cttccgggaa
      481 cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc tgatgcgccc
      541 aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct
      601 gttatcggtg tgtatgatgt gtttcggatc gctggttatc gcctgcctcc caggttatga
      661 aactataggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc agggattatc
      721 cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg
      781 caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac tgctagccct
      841 actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag agtggggatg
      901 gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt tacgtcgtca
      961 attagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat ctctgaaagg
     1021 attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct
     1081 ttgtttctat accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca
     1141 tgccaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc
     1201 actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc
     1261 gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt cctcgcctta
     1321 tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata catcaatcag
     1381 tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc
     1441 atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt tgtcgctgaa
     1501 atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt
     1561 tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt gacgggtcag
     1621 ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat ccttccagct
     1681 ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca aactgacaga
     1741 gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc
     1801 atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct
     1861 ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag
     1921 aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag
     1981 gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac
     2041 cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga tggtggtatg
     2101 ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agctccagtt gtggatcgtg
     2161 gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt
     2221 gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc
     2281 tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac ggctcaggaa
     2341 aagacgcaga ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc
     2401 ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga cctttttccc
     2461 ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga agtatggcgt
     2521 acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct tgttcaacag
     2581 actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc
     2641 ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat cgttcttgat
     2701 ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat
     2761 attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga taagatgata
     2821 acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct
     2881 ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg
     2941 ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct gcgccatgcg
     3001 gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg
     3061 atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag ctttatacaa
     3121 cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga caatgcaaat
     3181 gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg cgggagcgta
     3241 aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg
     3301 atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag
     3361 gatcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt
     3421 agtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt tagcgtaagc
     3481 ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggctagcgcc
     3541 accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc
     3601 gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt gagatccgag
     3661 cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc acgcgtgacg
     3721 ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat ttcccgatcg
     3781 tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag
     3841 tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag ttgtggctca
     3901 tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct
     3961 tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc ttcaaacggc
     4021 gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga
     4081 atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc
     4141 tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt
     4201 ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg
     4261 acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg
     4321 aaaaccggat cgtgcccaac cacaacccgc aactcctgag cgccagcacg gttagccata
     4381 ctttgcacca acaggccgtg gatccgcgcc tgcggccagg ccatttttac gcgatcgaaa
     4441 atggcgtttg ccgcttgctg gacttcattg gctgtacgca agtacagcat gacgccctga
     4501 acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg ataaccaatc
     4561 tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg gagcgtgttc
     4621 atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg
     4681 gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc
     4741 aacgccggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat atgcataaaa
     4801 gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct gaataaacgt
     4861 cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg
     4921 cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg
     4981 gcgctgggcg aatgaataac catcagcgcg tcaaaatcct ggctgtggag cagaatatcc
     5041 agcgttttaa tatagtgctc actgctggcg tcatcgcgta gatcgagcgg gttagatatt
     5101 gccacatgtt ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc
     5161 agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc agcaccgttg
     5221 ctgataatca tcagccggtc gccatgtagc gggcgcatat ggctaagggt ttccaccgcc
     5281 gaaaacagct cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agccgcatcc
     5341 catgccgggt ccattcctgc cgtggtgttg agcagtcgct gtgccgccgg gctacgtccg
     5401 cttttaatca ccagaatcgg tttattacgc gaggcactac gggccgccga aacaaagcgt
     5461 cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgtgcc
     5521 agatagtcaa gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag
     5581 gaaaagccca tcttacgctg ttgcgcccag tcgaggatgg tgttggagac ggcagccgat
     5641 tgcgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga
     5701 ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat gttatggcgc
     5761 agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt
     5821 ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat
     5881 aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc caacacaccc
     5941 aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc
     6001 aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc aattaccgct
     6061 atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga
     6121 agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac ccagatgttg
     6181 cgttttccct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgctgc
     6241 cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg
     6301 gccttcagcc agggcttcac gcaggcgata ggcagaaagc cgggaaagat cgacggaaat
     6361 gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt ccggccaggt
     6421 accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga tcacttcccg
     6481 ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg ggttttgtac
     6541 cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg
     6601 caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat
     6661 cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt
     6721 ctctggcaaa agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg
     6781 tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gtgttttcgg tcataagaga
     6841 gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa
     6901 gattaaagag attcgttcag ccagctatcg aacggagctt tcggtactgc gccattaagc
     6961 atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag
     7021 cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta
     7081 cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt
     7141 gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag tgtttcaccg
     7201 gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca
     7261 tcttcgatcc gatcgtcggg aatgcgattg atggactgac aatgggtaca aacggtattc
     7321 ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat
     7381 atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc aataggtatg
     7441 atagtcgcag gctttggtac gaaatcatgg agaaccgcgc ggacttcggg taatctgcgc
     7501 gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa
     7561 ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg
     7621 taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc
     7681 ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc cgtggcgcac
     7741 ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg
     7801 taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa cccgcgtcta
     7861 cggcgaaaac gcctgtcagg cactgttcca gagccgtccg gaagcaattg ttcgcgcctg
     7921 gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg
     7981 caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca cggaacatca
     8041 cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag
     8101 ccaggcaggc gcgcaggatt gcgttctggc actggaaaac gaatctaacc cgcataacct
     8161 gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg tgcaggatgc
     8221 ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag agctcgttca
     8281 gtcgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg caggttacac
     8341 cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc tgccagcgaa
     8401 aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa
     8461 cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata tctctgtcgc
     8521 aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa tcagccgggt
     8581 ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg gtaatactgg
     8641 catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg
     8701 gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac
     8761 atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa
     8821 aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag taaacgtctc
     8881 ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca cagtattgag
     8941 cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc gggtgaagcc
     9001 cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa tggcaatgcc
     9061 gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag gatcctggcc
     9121 gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa agacatcttt
     9181 ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt
     9241 aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta attcgttagc
     9301 catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt ctacagaaca
     9361 aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg gtaaaatttc
     9421 acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat cgatgtgtgg
     9481 tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata cactcaagca
     9541 acaatggttt taccaattag ccgcgacagg ctgaacaaat caaataattt tgccggggag
     9601 gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc tgctgaactc
     9661 tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa aagcaggtta
     9721 tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc gtgaagttcc
     9781 agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg ttaacgttct
     9841 gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc agctgaccat
     9901 ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc agcgcgacgt
     9961 tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac taagcggaag
    10021 agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca atagaccagc
    10081 aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg ctgaaacaga
    10141 tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag caaaacgctg
    10201 acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga cagtgccgta
    10261 acaccgtaca aaatga
//
LOCUS       DAJTSL010000143        10272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTSL010000143 DAJTSL010000000
VERSION     DAJTSL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.143"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OO433_003259"
     CDS             complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OO433_003259"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6051623.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1374..1787
                     /gene="msrB"
                     /locus_tag="OO433_003260"
     CDS             1374..1787
                     /gene="msrB"
                     /locus_tag="OO433_003260"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6051624.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1829..2101
                     /locus_tag="OO433_003261"
     CDS             1829..2101
                     /locus_tag="OO433_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6051625.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OO433_003262"
     CDS             complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OO433_003262"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6051626.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OO433_003263"
     CDS             complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OO433_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6051627.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OO433_003264"
     CDS             complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OO433_003264"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6051628.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDHNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPSAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6114..6665
                     /locus_tag="OO433_003265"
     CDS             6114..6665
                     /locus_tag="OO433_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6051629.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAILEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6782..7825
                     /gene="selD"
                     /locus_tag="OO433_003266"
     CDS             6782..7825
                     /gene="selD"
                     /locus_tag="OO433_003266"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6051630.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQACVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7830..9791
                     /gene="topB"
                     /locus_tag="OO433_003267"
     CDS             7830..9791
                     /gene="topB"
                     /locus_tag="OO433_003267"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6051631.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            9918..>10272
                     /locus_tag="OO433_003268"
     CDS             9918..>10272
                     /locus_tag="OO433_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051632.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQ"
BASE COUNT         2583 a         2508 c         2716 g         2465 t
ORIGIN      
        1 gaagtcgctc tttttagatc acagtttcat ctcaacttat ttggagatgt gagcgatcag
       61 gtccagaact ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa
      121 gttgtcgttc agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt
      181 gaaatcggta gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc
      241 agcagcggct ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac
      301 ggtcaggtca actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt
      361 gccattcagt tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat
      421 gatgttctgg gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt
      481 tttctgagta gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt
      541 atcgttgata actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac
      601 gatgtcctgg ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc
      661 tttagacgga ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt
      721 ttcgtcagtc aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca
      781 tttcaggtta gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag
      841 atgaccgtct ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag
      901 catgtatgcc atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga
      961 acgtttctga gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac
     1021 tttgatagtc atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt
     1081 acaaaaacct tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg
     1141 tgcctaagca ttacgcgact gactcgcctc actcttcctt tgggcttgaa accacatggg
     1201 gtcggcgccc cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta
     1261 ggtgcagcca tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt
     1321 tttgttagaa tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta
     1381 ataaaccttc ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc
     1441 agaatcatgg gacagagccg ccatttacgg ggcgtttact gcataacaag cgtgacggcg
     1501 tatatcactg tttgatctgc gatgccccgc tgtttcattc cgaaaccaag tatgattccg
     1561 gctgtggctg gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag
     1621 acttgtcaca tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg
     1681 ggcatgtctt tcccgacggg ccgcaaccaa caggcgaacg ttattgtgtt aactctgctt
     1741 ctttacgctt cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag
     1801 caacttattc cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac
     1861 gcctgaggta taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ctgatggcgt
     1921 tgcgttgacc gaggaacaaa aagagaattg cctgcaactg gtgatgctgt ggcaagcccg
     1981 ccataatacc gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag
     2041 caaacagcag ttaaaagaag attttggtat ctcagcaaag cctattgcta tgtttaagtg
     2101 aatgagttat ctagtattta agcctgttat taagggaggg caaatatact tgttcggacg
     2161 tcgtgtttta gctgcaagta ggttaaatgc aacactgaca agtattatct ggcctacaat
     2221 gtcattgagg aaatgtaggc cagagatgaa ttcaactaat attacccctg tgtctcttcc
     2281 cagtctgcca gcgtatatag cgttgcccca gctgctgaca tctccataaa cgcgtgtgca
     2341 ctgtcctggg gctggatatt cacgccacga caaccatcgg taatcacgtt caccttataa
     2401 cctaactgta acgcgtccag cacggtaaac ttcacgcaat agtcagtagc caggcccata
     2461 acgatcaatt catcgatttc atgatcgcgt aaccagtcat cgagagaggt tttctgccga
     2521 cggccgttat caaaaaaggc actgtaactg tcaactaaag gattttcgcc tttatggaac
     2581 accgctgcga tcgctttttg gttcagtaac ggatgtaatt gtgcgccttc actgttctgc
     2641 acacagtgat ctggccagaa ggtttgtggc aaaccgtcga gttggcctgg cgtataaggc
     2701 tctacaccgt gctgactggc aaaactgccg tgattcgccg gatgccagtc ctggctggcg
     2761 ataaccgctt ctccgcgcga ctggcaccag tcaataaggc ggttagcaac ctccaccgta
     2821 ctgtcacctt ccggtacggc gagcgcgcca ccagcacaga aatcattttg taaatcgacc
     2881 agtaacaggg cgcgatgggg cattgcagtc tccttaatca tccggcgtca gttcgccgcg
     2941 caggttttgg ctcatggcct tgcgaatggt ttcagtatcc agttcctggc tcagcaggta
     3001 atgcagtttg gttagcgtgg cttctacagt catatctgca ccgccaataa cgccggcatg
     3061 ggcgagggcg ttaccggtgg cataaccacc catattcact ttaccggaca tacattgtgt
     3121 caggttgacc accacaatac cgcgatcgct ggcttcttgt aattcctgca ggaaggcttt
     3181 gttttgtggc gcattaccca cgccatagga gcgcagaatc aatgctttca ctggttggcg
     3241 cagaaaattg cgcacgacat cagcagaaat ccccggataa atcgtcacta cgccaattgg
     3301 ttgtggcgtg attggatgaa cgattaattc cccttcaccg tgcggggcgg gtggcgtatt
     3361 caaacgacga atatggatac cagcttccag taacggagga aggtttggag aggcaaacgc
     3421 atcaaaacca tcggcatggg ctttggtagt gcggttgccg cgatacaatc ggttattgaa
     3481 aaagagcgtt acttcgttga tcggataatt cgccgcaacg tacaacgcat tcagcagatt
     3541 aatttgtccg tcagagcgta actcagccag cgggatttgt gaccctgtca caatgaccgg
     3601 tttaccgaga ttctcgagca tgaacgacag cgcagaggcg gtatacgcca tcgtgtcggt
     3661 accgtgtaga atgacaaaac catcatagtc gtcatagtgc gctttaatat cttcagcaat
     3721 atgctgccag tcatccggcg tcatatcaga agaatccatc aacggcgtat attcatgaat
     3781 ggtgaaatct ggcatctccg ggcgatggaa ttccggcatc agcgccagtt ggcgttgtag
     3841 atgacctgac accggaatat aaccctgctc ggaacgctgc atcccgatgg tcccgcccgt
     3901 gtaggcgacg taaattgatt tcttttgcat gatattgata tgaatgatat cgaaagagca
     3961 aaagtatata cgtgaggcag gaggagcgca cctgatacta tgcggataag gaaatatggg
     4021 gcgagacagc gggaagagga ttcgtcgagg taaaaaataa tgtggccgac gatctgtcgg
     4081 ccactcagta caagacttaa cgcacgttgg cgcaggtcag gcaaaacgca taacggtttt
     4141 gtgggtcgtt aaacgcggcc agcttgtcgc tttcactttt aacggtagag gctaccgagg
     4201 caagcggtgc aggtaacatg gcctggaacg catctggcaa cattgcccgg acagaaccag
     4261 acatgttgtc catcactttg tcgaagaagg tcggttcatc aacgtagtat tccagatgcc
     4321 actgtttcac ttttgcgagc tctgctgctt tggcaaccgc atcatcgaaa tccccgagac
     4381 tatcgaccag cccgttagct tttgcatcct gaccggtcca gacgtggccc tgggcgattt
     4441 tatcaatttg ctccggcgtc gaatgacgcg catcagcaac cagcgtgata aagcgtttat
     4501 agccattctc aatgcttaat tgcatcatct gctgcgcttc cggcggcagt gccctggtga
     4561 tagaaacatc cgccagcggt gaagttgaga caccatcagt atgaacacca atcgaatcca
     4621 gactattttc tacggtggtg atcacgccga agataccgat agaaccggtc agggtgctgg
     4681 ggttagccac aatgtaatta gctggcgtgg aaatccagta accaccagat gccgccatgc
     4741 cgcccatcga tacaaccaca ggcttacccg ctgcccgggc tgctgccagt tcagcgcgaa
     4801 tcacttcaga cgcggtaacg ctgccgcctg ggctattaac acgcaggaca atcgctttca
     4861 ctttcgggtc aaggcgagcg tcgcggattt gtgccgcagt ggtatcaccg ccaacattcc
     4921 cctgagtttc ctcgccatcc ataattgcgc cattagcgaa gacgacaccg atgctgtcac
     4981 cggtatctgc cggcgttttc aatgcgtaat cgtaataact gatggcgcga taatttttat
     5041 cagtcttact ccagccgaac tctttggtca gagctttttc gatttcggca ctcgatgcca
     5101 gtgcatcgac cagcttgttt tccagtgcat atttcgcggt atcgccaccg gttttggtta
     5161 aaccctcaag caacccttgc gcgctaggga atacctgctg agcagggatc tgtcggttag
     5221 cggcaacagt attcagatag ttttgccaca gctcaccaat ccagcggctg tcagcttcgc
     5281 gggctgccgg tgacatatca tcacgaataa acggttcaac ggcagattta tacgtaccca
     5341 cgcggaacac atgggtggaa actttcagct tatccagcaa cgatttgtag tacagaccgt
     5401 tggtagcaaa accgtgcaga tcaaccacgc cttgcggtga cagccaaatt ttattggcga
     5461 aactggcgag ataatattgc ccctggctgt agttctcgcc aacggcataa accggtttcc
     5521 cgctgtcacg aaactctttc agggctttgc cgatgtactg catagacggc tggtcgccgc
     5581 ctgcgaagtt tttcagatcc atcacgatac cggtgatatt gtggtcgtcc ttcgcctggc
     5641 gaatggtgtt gacgatatca aacagtgagt tttcctgcag acgatcggaa ctggcaccaa
     5701 gcagctggcg gcttaattta ctaaaccgct gagaactgtc gggtttatcg acgatcacac
     5761 cggaaatgtc cagcagcagt gcgccacgac tggccgtttc tttcgaatca ccaccactga
     5821 cctgcatcca gatccccaca ccaaccagta cgaggaaaat aaagaacagg ttaagtacca
     5881 tttcacggac gaaattcagc agacgccacg tccatttaaa aaatccggca ataaatcgcc
     5941 aaagggttcg catgtattct cccaacttaa ggtcacacct gtcacaggcg caatagatgt
     6001 ggttatcgta atgacccaac cgacacttgt cagcaggaat tgcggactgt gctgtaacaa
     6061 aatccactct cgtgttaatt ttgtgaataa atatcacgac aggagttaat caaatggatg
     6121 cactcgaact attgatcaat cgccgtagcg cctcccgcct ggctgaaccc gcgccaacgg
     6181 gtgaacaact gcaaaacatc ctgcgtgcgg gtatgcgtgc gccggaccat aagtccatgc
     6241 aaccgtggca tttttttgtg attgaagggg aagggcgcga gcgtttcagc gccatactgg
     6301 aacagggggc gattgctgcc ggtagtgatg acaaagctat cgacaaagcc cgtaatgcgc
     6361 cgttccgcgc accgctcatc atcacggtgg tggcgaaatg cgaagaaaat cataaagtcc
     6421 cgcgatggga acaggaaatg tctgccggat gcgcggtcat ggcgatgcaa atggcagccg
     6481 ttgcccaggg atttggcggc atctggcgca gtggcgcatt aactgaaagt ccggtagtgc
     6541 gtgaagcatt cggttgccgt gagcaggata aaattgtcgg ttttctctac ctcggcacgc
     6601 cgcagttgaa agcatctacg tcgattaacg tcccggaccc gacgccgttt gtaacttatt
     6661 tctgataatt gtcgcgaaac tgtctggatt gtgagcaaag gcgcaggaat tcagacactc
     6721 tcacttatca cttcacggaa tgagggttac catacccgga ttgcattgac aggagatgtc
     6781 catgagcgag aactcgattc gtttgaccca atacagccac ggagctggtt gcggctgtaa
     6841 aatttcccca aaagtgttgg aaaccatcct gcacagtgag caggcgaagt ttgttgatcc
     6901 gaatttgctt gtgggtaatg agacccgcga cgatgcggcg gtgtacgatc tgggcaatgg
     6961 caccagcgtt atcagtacca ccgacttctt tatgccgatc gttgataatc ctttcgattt
     7021 tggccgcatt gcggcgacta acgccatcag cgatatcttc gcgatggggg gcaaaccgat
     7081 tatggcgatt gcgatcctcg gctggccgat taacaaactt tccccggaaa ttgcccgcga
     7141 agtaaccgaa ggtggacgct atgcatgccg tcaggcgggt attgcgctgg ctggcggtca
     7201 ctccatcgat gcgccggagc cgatttttgg tctggcggta acggggatcg taccgaccga
     7261 gcgggtgaag aaaaacagca ccgcacaagc cggatgcaaa ctgttcctga cgaaaccgct
     7321 ggggatcggc gttcttacca cggctgagaa aaaatcactg ctgaaaccag aacatcaggg
     7381 actagcgacg gaagtgatgt gccggatgaa catcgcaggc gcgtcctttg ccaacatcga
     7441 aggcgtaaaa gcgatgaccg acgttacggg ctttggtctg ctgggccatt tgagcgaaat
     7501 gtgtcagggc gcaggtgtgc aggcgtgcgt tgactatgac gcgatcccaa aattgccggg
     7561 ggttgaagag tacattaagt tgggcgcagt gcctggcggc accgaacgta actttgccag
     7621 ctacggtcat ctgatgggtg aaatgccgcg tgaagtgcgc gatttgctgt gcgatccgca
     7681 aacttctggc ggtttgctgc tggcggtcat gccggaagca gaaaatgagg tcaaagctac
     7741 agccgccgag tttggcattg aactgacggc aattggcgaa ctggtgccag cgcgcggcgg
     7801 tcgtgccatg gttgagattc gttaattcaa tgcggttgtt tattgccgaa aaaccgagtc
     7861 tggcgcgcgc cattgctgat gtcctgccca aaccgcaccg gaaaggcgat ggctttatcg
     7921 agtgcggtaa tggtcaggtg gtgacctggt gtatcggtca cctgcttgag caggcgcagc
     7981 cagacgccta cgacagccgc tatgcgcgct ggaatcttgc ggatttgccg attgtcccgg
     8041 aaaagtggca attacagccc cgaccctccg tgaccaaaca acttaacgtc atcaaacgct
     8101 tcctgcatga agccagcgaa atcgttcacg ccggggaccc ggatcgtgaa gggcaactgc
     8161 tggtggatga agtgctggac tatctgcaac tggcaccgga aaagcgccag caggtgcagc
     8221 gttgcttgat aaacgacctg aacccgcagg cggttgagcg ggcgatcgac cgtcttcggt
     8281 ccaacagtga gtttgtgcca ttgtgcgttt ctgcgctggc gcgagcgcgt gccgactggc
     8341 tgtacggcat caatatgacc cgtgcgtata ccattctcgg tcgcaatgcc ggttatcagg
     8401 gcgtactttc cgtgggacgc gtgcagacac ccgtgctcgg gctggtggtg cgccgcgatg
     8461 aagagattga aaacttcgtg gcgaaagact tctttgaagt caaagcacat atcgtgacac
     8521 ctgccgatga gcggtttacc gctatctggc aaccgagcga agcgtgtgaa ccgtaccagg
     8581 atgaagaagg gcgcttgtta catcgtccac tggcggagca tgtggttaac cgcattagtg
     8641 gtcaaccagc aattgtcacc agctataacg ataaacggga atcagaatcc gcgccgctgc
     8701 ctttttcgct ttcggcgttg cagattgaag cggcaaaacg ctttggtctg agcgcgcaga
     8761 acgtgcttga tatctgccag aagctgtacg aaacgcacaa gctaatcact tatccgcgtt
     8821 ccgattgtcg ctatttgcca gaagaacatt ttgccggacg ccacgcggtg atgaatgcca
     8881 tcagcgttca tgcaccagat ctgttgccgc aaccagtggt agatccagat atacgcaacc
     8941 gctgttggga tgacaaaaag gtcgatgcac accacgccat catcccgacc gcacggagtt
     9001 ctgcgatcaa cctgacggag aacgaagcga aggtctataa cctgattgcc cgtcagtact
     9061 tgatgcagtt ctgcccggat gcggtgttcc gcaagtgtgt tatcgaactg gacattgcca
     9121 aaggcaaatt tgtcgctaaa gcgcgttttc ttgctgaagc aggctggcgc acgctgttag
     9181 gcagcaaaga gcgcgatgaa gaaaacgacg gtacgccatt gcctgtggtg gcgaaaggcg
     9241 atgagttgct gtgtgaaaaa ggtgaagtgg tagagcggca aacccagccg ccgcgccatt
     9301 ttaccgatgc aacactgctt tcggcgatga ccgggatcgc gcgctttgtg caggacaaag
     9361 atctgaaaaa gatccttcgt gcgaccgatg gtctggggac agaagcaacc cgtgccggga
     9421 ttattgaact gctgttcaag cgtggttttc tgaccaaaaa agggcgctat atccactcta
     9481 ccgacgccgg aaaagcgtta ttccattcgc tgccagaaat ggcgacgcga ccggacatga
     9541 ccgcgcactg ggaatcggtg ctgacgcaaa tcagcgaaaa gcagtgtcgc tatcaggact
     9601 ttatgcagcc gctggtgggg acgctatatc agcttattga tcaagccaaa cgtacgccgg
     9661 tgcggcagtt tcgcggcatt gtggctccgg gcagtggtgg cagtgctgat aagaaaaagg
     9721 ctgcaccgcg taaacgtagt gcgaaaaaaa gtccgccagc agatgaagtc ggaagcgggg
     9781 cgatagcgta agcgagtgaa tctttcgtgc tattcgagtc atattctgaa atatccagcg
     9841 aatcaagaaa attcgttgga tatttttttg catggataaa attatcgcct ctaaagtatg
     9901 taataacagg gaatgtgatg aaaaaagttt tattacaaaa ccatcctggg agcgagaagt
     9961 attcttttaa tggctgggaa atatttaata gtaattttga acggatgatt aaagaaaata
    10021 aggccatgct gctttgtaag tgggggtttt atttaacatg tgttgtcgct gtaatgtttg
    10081 tgttcgcagc gataacatcc aacggtttga atgaaagagg cctgattacc gcgggatgct
    10141 cttttcttta tctattaatt atgatggggc ttattgttcg ggccggtttt aaagcaaaaa
    10201 aagaacaact gcattattat caggctaaag gtattgagcc gctcagtatc gagaagttac
    10261 aggcgctaca at
//
LOCUS       DAJTSL010000144        10213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTSL010000144 DAJTSL010000000
VERSION     DAJTSL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.144"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            141..1997
                     /gene="kup"
                     /locus_tag="OO433_003269"
     CDS             141..1997
                     /gene="kup"
                     /locus_tag="OO433_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6051633.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSLTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2164..2583
                     /gene="rbsD"
                     /locus_tag="OO433_003270"
     CDS             2164..2583
                     /gene="rbsD"
                     /locus_tag="OO433_003270"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6051634.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2591..4060
                     /gene="rbsA"
                     /locus_tag="OO433_003271"
                     /pseudo
     CDS             2591..4060
                     /gene="rbsA"
                     /locus_tag="OO433_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            4065..5030
                     /gene="rbsC"
                     /locus_tag="OO433_003272"
     CDS             4065..5030
                     /gene="rbsC"
                     /locus_tag="OO433_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6051635.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGTIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5055..5941
                     /gene="rbsB"
                     /locus_tag="OO433_003273"
                     /pseudo
     CDS             5055..5941
                     /gene="rbsB"
                     /locus_tag="OO433_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6067..6996
                     /gene="rbsK"
                     /locus_tag="OO433_003274"
     CDS             6067..6996
                     /gene="rbsK"
                     /locus_tag="OO433_003274"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6051636.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7000..7992
                     /gene="rbsR"
                     /locus_tag="OO433_003275"
     CDS             7000..7992
                     /gene="rbsR"
                     /locus_tag="OO433_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6051637.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(7958..9385)
                     /gene="mdtD"
                     /locus_tag="OO433_003276"
     CDS             complement(7958..9385)
                     /gene="mdtD"
                     /locus_tag="OO433_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6051638.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     RATWHWIFLINIPIGIAGLLYARKHMPNFTTAQRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9408..10100)
                     /locus_tag="OO433_003277"
     CDS             complement(9408..10100)
                     /locus_tag="OO433_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6051639.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKKNFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KTCQALLRSPDK"
BASE COUNT         2486 a         2458 c         2702 g         2567 t
ORIGIN      
        1 aaggggggta tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaaacat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatcca atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgttgt cgattacggg ggtcgaggcg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgctgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccgctgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtgct gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggtt ggttgccatt gagcctcggt acggtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgtaccgaag
     1681 acgcgccata tgtccataat gtccgtcggg tacagattga acaactgtcg ctcactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgaaacg cccgtggtat ttgcgtctgc gcggcaagct gtacttgctg ctgcaacgta
     1921 atgcgctgcg tgcaccagat caatttgaaa tcccgccaaa cagggttatc gaactgggta
     1981 ctcaggtcga aatctaacgc cagacgcctc ctttctccat aagggggcgt ttttgttttc
     2041 atggttaatc accatgtaaa acgtttcgag gttgatcaca tttccgtaac gtcacgatgg
     2101 ttttcccaac tcagtcagga ttaaactgcg ggtcagcgaa acgtttcgct gatggagaaa
     2161 aaaatgaaaa aaggcaccgt tcttaattct gatatttcat cggtgatctc ccgtctggga
     2221 cataccgata cgctggtggt gtgtgatgct ggtttaccca tccccaaaag tacaacgcgt
     2281 atcgatatgg cattaaccca gggtgtacct tcttttatgc aggtgctggg cgtcgttaca
     2341 aatgaaatgc aggtcgaggc tgccattatc gcggaagaga tcaaacaaca taatccgcaa
     2401 ctccacgaaa cgttgctcac tcaccttgag cagctgcaaa aacaccaggg aaataccatt
     2461 gaaattcgtt acaccacgca tgaacaattc aaacaacaaa ccgcagaaag tcaggcggta
     2521 attcgcagcg gagaatgttc tccgtatgcg aatatcattc tctgtgctgg cgtgacgttc
     2581 tgaggccgtc atggaagcat tacttcagct taaaggcatc gataaagcct tcccgggcgt
     2641 aaaagccctc tcgggcgctg cgttaaatgt ctatccgggc cgcgtgatgg cgctggtggg
     2701 cgaaaacggc gcgggtaaat ccaccatgat gaaagtgctt actggtatct atgctcgcga
     2761 tgccggcacg cttttatggc tggggaaaga aacgacattt accgggccga aatcttccca
     2821 ggaagccggg attgggatta tccatcagga actgaacctg atcccgcagt tgaccattgc
     2881 cgaaaacatt ttcctcggtc gtgagtttgt taatcgcttt ggcaaaattg actggaaaac
     2941 catgtatgcc gaagcggata aattgctggc taaacttaac ctgcgcttta aaagcgacaa
     3001 gctggtgggc gatctttcca tcggtgacca gcaaatggtt gaaatcgcca aagtgctgag
     3061 ctttgagtcg aaagtcatca ttatggatga accgaccgat gcgctgaccg ataccgaaac
     3121 cgaatccctg ttccgcgtca tccgcgaact aaaatcgcaa ggccgcggta ttgtctatat
     3181 ctcccaccgc atgaaagaaa tcttcgagat ttgcgatgcc gttaccgttt ttcgtgacgg
     3241 gcaatttatt gctgagcgcg aagtggcatc actgaccgaa gattcgctga ttgagatgat
     3301 ggtgggtcgc aagctggaag atcaatatcc acacctggac aaagcgccgg gagatatccg
     3361 cctgaaagtc gataatctct gcggacctgg cgttaacgat gtctctttta ctttacgcaa
     3421 aggcgaaatt cttggcgtct ctggtttgat gggcgcaggt cgtaccgaac tgatgaaagt
     3481 gctctacggc gcactaccgc gcaccagcgg ttacgtcacc ctggatgggc atgaagtcgt
     3541 tacccgttca ccgcaggatg gcctggcaaa cggcattgtg tatatctccg aagaccgtaa
     3601 acgtgacggt ttagtgttgg gcatgtcagt aaaagagaac atgtcgctga cagcgctgcg
     3661 ctacttcagc cgcgctggcg gcagtttgaa gcatgccgat gaacagtagg ctgtgagtga
     3721 tttcattcgt ctgtttaatg tgaaaacacc gtcgatggaa caggcaattg gtctgctttc
     3781 cggtggcaat cagcaaaaag tgttgatcct tgatgaacct acccgtggcg tagatgtcgg
     3841 cgcgaaaaaa gagatctatc aactgattaa ccagttcaaa gccgatggct tgagcatcat
     3901 tctggtgtca tcggagatgc cagaagtatt aggcatgagc gatcgcatca tcgtcatgca
     3961 tgaagggcat ctcagcgggg aatttactcg tgagcaggcc acccaggaag tgttaatggc
     4021 tgccgctgtg ggcaagctta atcgcgtgaa tcaggagtaa aaaaatgaca acccagactg
     4081 tctctggtcg ccgttatttc acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc
     4141 tgctggtgct gatcgcgatt gtctcgacgt taagcccgaa ctttttcacc atcaataact
     4201 tattcaatat tctccagcaa acctcggtga acgccattat ggcggtcggg atgacgctgg
     4261 tgatcctgac ttcgggcatc gacttgtctg taggttctct gttggcgcta accggcgcag
     4321 ttgctgcatc tatcgtcggc attgaagtca atgcgctggt ggctgtcgct gctgctctcg
     4381 cgttaggtgc cgcaattggt gcggtaaccg gggtgattgt agcgaaaggt cgcgttcagg
     4441 cgtttatcgc tacgctggtt atgatgcttt tactgcgcgg cgtgaccatg gtttatacca
     4501 acggtagccc agtgaatacc ggctttactg agaacgccga tctgtttggc tggtttggta
     4561 ttggtcgtcc gctgggcgta ccgacgccag tctggatcat ggggattgtc ttcctcgcgg
     4621 cctggtacat gctgcatcac acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg
     4681 aagcggcaac gcgtctttct ggtatcaacg tcaataaaat caaaatcatc gtctattctc
     4741 tttgtggtct gctggcatcg ctggccggga ccattgaagt ggcgcgtctc tcctccgcac
     4801 aacccacggc ggggactggc tatgagctgg atgctattgc tgcggtggtt ctgggcggta
     4861 cgagtctggc gggcggaaaa ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg
     4921 gcttccttaa taatggattg aatttgttag gtgtttcctc ctattaccag atgatcgtca
     4981 aagcggtggt gattttgctg gcggtgctgg tagacaacaa aaagcagtaa taacgactac
     5041 aggacatctt gaatatgaac atgaaaaaac tggctaccct ggtttccgct gttgcgctaa
     5101 gcgccaccgt cagtgcgaat gcgatggcaa aagacaccat cgcgctggtg gtctccacgc
     5161 ttaacaaccc gttctttgta tcgctgaaag atggcgcgca gaaagaggcg gataagcttg
     5221 gctataacct ggtggtgctg gactcccaga acaacccggc gaaagagctg gcgaacgtgc
     5281 aggacttaac cgttcgcggc accaaaattc tgctgattaa cccgaccgac tccgacgcag
     5341 taggtaatgc tgtgaagatg gctaaccagg cgaacatccc ggttatcact cttgaccgcc
     5401 aggcaacgaa aggtgaagtg gtgagccaca ttgcttctga taacgtactg ggtggcaaaa
     5461 tcgccggtga ttacatcgcg aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag
     5521 gcattgctgg tacatccgca gcccgtgaac gtggcgaagg cttccagcag gccgttgctg
     5581 ctcacaagtt taatgttctt gccagccagc agattttgat cgcactaaag gtttgaacgt
     5641 aatgcagaac ctgttgaccg ctcatccgga tgttcaggct gtattcgcgc agaatgatga
     5701 aatggcgctg ggcgcgctgc gcgcactgca aactgccggt aaatcggatg tgatggtcgt
     5761 cggatttgac ggtacaccgg atggcgaaaa agcggtgaat gatggcaaac tagcagcgac
     5821 tatcgctcag ctacccgatc agattggcgc gaaaggcgtc gaaaccgcag ataaagtgct
     5881 gaaaggcgag aaagttcagg ctaagtatcc ggttgatctg aaactggttg ttaagcagta
     5941 gttttaatca ggttgtatga cctgatggtg acataaatac gtcatcgaca gatgaacgtg
     6001 taatataaag aaaagcaggg cacgcgccac cctaacacgg tggcgcattt tatggacatc
     6061 ccgaatatgc aaaacgcagg cagcctcgtt gttcttggca gcattaatgc tgaccacatt
     6121 cttaatcttc aatcttttcc tactccaggc gaaaccgtaa ccggtaacca ctatcaggtt
     6181 gcatttggcg gcaaaggcgc gaatcaggct gtggctgctg ggcgtagcgg tgcgaatatc
     6241 gcgtttattg cctgtacggg tgatgacagc attggtgaga gcgttcgcca gcagctcgcc
     6301 actgataaca ttgatattac tccggtcagc gtgatcaaag gcgaatcaac aggtgtggcg
     6361 ctgatttttg ttaatggcga aggtgagaat gtcatcggta ttcatgccgg cgctaatgct
     6421 gccctttccc cggcgctggt ggaagcgcaa cgtgagcgta ttgccaacgc gtcagcatta
     6481 ttaatgcagc tggaatcacc actcgaaagt gtgatggcag cggcgaaaat cgcccatcaa
     6541 aataagacta tcgttgcgct taacccggct ccggctcgcg aacttcctga cgaactgctg
     6601 gcgctggtgg acattattac gccaaacgaa acggaagcag aaaagctcac cggtattcgt
     6661 gttgaaaatg atgaagatgc agcgaaggcg gcgcaggtac tgcatgaaaa aggtatccgt
     6721 actgtactga ttactttagg aagtcgtggt gtatgggcta gcgtgaatgg tgaaggtcag
     6781 cgcgttcctg gattccgggt gcaggctgtc gataccattg ctgccggaga tacctttaac
     6841 ggcgcgttaa tcacggcgtt gctggaagaa aaaccattgc cagaggcgat tcggtttgcc
     6901 catgctgcag ctgcgattgc cgtaacacgt aaaggcgcac aaccttccgt accgtggcgt
     6961 gaagagatcg acgcattttt agacaggcag aggtgacgct tggctacaat gaaagatgtt
     7021 gcccgcctgg cgggcgtttc tacctcaaca gtttctcacg ttatcaataa agatcgcttc
     7081 gtcagtgaag cgattaccgc caaagttgaa gcggcgatta aagaactcaa ttacgcgcca
     7141 tcagccctgg cgcgtagcct caaactcaat caaacacata ccattggcat gttgatcact
     7201 gccagtacca atcctttcta ttcagaactg gtgcgtggcg ttgaacgcag ctgcttcgaa
     7261 cgcggttata gtctcgtcct ttgcaatacc gaaggcgatg aacagcggat gaatcgcaat
     7321 ctggaaacgc tgatgcaaaa acgcgttgat ggcttgctgt tactgtgcac cgaaacgcat
     7381 caaccttcgc gtgaaatcat gcaacgttat ccgacagtgc ctactgtgat gatggactgg
     7441 gctccgttcg atggcgacag cgatcttatt caggataact cgttgctggg cggagactta
     7501 gcaacgcaat atctgatcga taaaggtcat acccgtatcg cctgtattac cggtccgctg
     7561 gataaaactc cggcgcgcct gcggttggaa ggttatcggg cggcgatgaa acgtgcgggt
     7621 ctcaacattc ctgatggcta tgaagtcact ggtgattttg aatttaacgg cgggtttgac
     7681 gccatgcgcc aactgttatc acatccgctg cgtcctcagg ccgtctttac cggaaatgac
     7741 gctatggctg ttggcgttta ccaggcgtta tatcaggcag agttacaggt tccgcaggat
     7801 atcgcggtga ttggctatga cgatatcgaa ctggcaagct ttatgacgcc accattaacc
     7861 actatccacc aaccgaaaga tgaactgggg gagctggcga ttgatgtact catccatcgg
     7921 ataacccagc cgacccttca gcaacaacga ttacaactta ctccgattct gatggaacgc
     7981 ggttcggctt agatttacgc tgtcttttga tcaaattatt accatcggtt gttttcagaa
     8041 gcatgaacat tgctgctgaa gcaacagtaa taatgcccat cgtgataaac gtatagtgga
     8101 attgttcgac agtcgttgtg ccttccatcc cttcataaac gcgaaggacg gccgcactta
     8161 cagcaacgcc taaactaatc gacagttgct gcgtgaccgc cagaacactg ttaccgctgc
     8221 tggcgttgtc atcggtcaga tcggcaagtg tgatggtatt catcgcggta aattgcgtcg
     8281 acatagccat ccctaatata aacaacggca agatcagcat ccatatcgcc attgccggtg
     8341 attgcaaaga gaactgagcg atcattagcc caataatcac cgtgatcccc actaacgtat
     8401 ggcgatagcc cagacgacgt aagacttggg taaccatcga ttttgcaata atggaaccta
     8461 acgctgtcgg tgccatcata cagccggcaa taaacgcctg ataaccaaat cctacctgta
     8521 acatcaatgg cataaggaac ggtacactac cggtccccag acgggttgca atattgccta
     8581 cgataccgat cgagaaagtg cgggttttaa ataaatctaa tgaaattaat gggtttggcg
     8641 tgtgtcgcgc atggagaata tagagaagca gtaacccgat gctggtgaca attaccgtca
     8701 aggcaatcca gctggcgaca atcttttccc cgaatagctc tattccgctt gagaagagaa
     8761 caaggctgag gccaaacagc aaaaagccag tgatatcgaa tctgcgttgt gcggtggtga
     8821 aattgggcat atgtttgcgc gcgtaaagaa ggcccgcaat acctatgggg atattgatta
     8881 aaaatatcca gtgccaggtt gccctggtga ccagcacgcc gccaagaacg gggcctaaaa
     8941 ttggccccac cagacccggc atggcgacaa aattcaatac aggaagaagt tcattacgag
     9001 gataagcacg cagtaaggcc agccgagcaa caggcatcat cattgcgccg cctatcccct
     9061 gaataacccg gaagacaacc agctgtggta gcgaattaga aagtgcgcag gccagagaac
     9121 ccaacgtgaa cagactcacg gcaagggtaa aaatgcgacg cgtaccgaag cgatcggcta
     9181 gccatccgct taccggaata aacatcgcca ccgtcagcgt ataactgatg atggctgatt
     9241 gcatcgcgag aggagaacga ttaaggctat gagcgattgc gggtaaggcg gtattcagaa
     9301 tagtggcatc aagtgcctgc atgaagaagg ccatcgccgc gatccacggc aaacccgcca
     9361 tactacgctt ctttttatcg ctcattcaat gtcctgttat cgggttatca cttatcaggt
     9421 gagcgtagca gcgcctgaca agttttaaat gccgcgtcgc catcgctttg gataatcgca
     9481 tcgacaatcg cctggtgcag atccagcttt atcactgtgt cgctggtaat tgacgtgaag
     9541 taagtgtgat aaaccgaatg gaatagcgag gcgaatgagg tcaaaaacgg attggcgctc
     9601 atttcataga tatgctcatg ccaggccata tcgacttcga tccagcgttc acggcgaaag
     9661 ttctttttta atgccgccat ttcggccatt aacgtattga gatgcgcctt ctgttccgcg
     9721 gtgccaaccg ttgctgccag taggcaggct tgcggctcca gacagatacg cataaccaga
     9781 aagtgatcga tgacctgatg aaagttctct tctgtcatcc accaggtaag caattcctga
     9841 tcaagaaaat tccagtttga ttgtggcatg acccgggtac caattcgcgg tcgcggtaaa
     9901 accatccctt ttgccgttaa cgttttgacc gcttcgcgta ccgctgtacg actcactcca
     9961 aattgctcgc ccagctcaat ctcaccaggc aaaatggtgc cgggttcata ttcacctttt
    10021 aagatgcgtt gcgccagctt ctcagccaga acatacgaaa ggtttttctg tgcagctaac
    10081 tgttgtgcgc ttaaaggcat tactaatctt cctttttctt tttattcctc cttagtatgc
    10141 caccaggaag tgtgattacg gttgcaaaaa cggcaaattg cttgttttat ggcacattaa
    10201 cggggctttt gct
//
LOCUS       DAJTSL010000145        10210 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTSL010000145 DAJTSL010000000
VERSION     DAJTSL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.145"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(79..228)
                     /gene="hokB"
                     /locus_tag="OO433_003278"
     CDS             complement(79..228)
                     /gene="hokB"
                     /locus_tag="OO433_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR6051640.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            627..2240
                     /gene="trg"
                     /locus_tag="OO433_003279"
                     /pseudo
     CDS             627..2240
                     /gene="trg"
                     /locus_tag="OO433_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(2278..3200)
                     /locus_tag="OO433_003280"
                     /pseudo
     CDS             complement(2278..3200)
                     /locus_tag="OO433_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3417..4760
                     /locus_tag="OO433_003281"
     CDS             3417..4760
                     /locus_tag="OO433_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6051641.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGPLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GGPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEYRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHLGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4985..6640
                     /gene="opgD"
                     /locus_tag="OO433_003282"
                     /gene_synonym="mdoD"
     CDS             4985..6640
                     /gene="opgD"
                     /locus_tag="OO433_003282"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6051642.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6780..7004
                     /locus_tag="OO433_003283"
     CDS             6780..7004
                     /locus_tag="OO433_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6051643.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGFDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKGV"
     gene            7067..7603
                     /gene="rimL"
                     /locus_tag="OO433_003284"
     CDS             7067..7603
                     /gene="rimL"
                     /locus_tag="OO433_003284"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6051644.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7598..8578)
                     /locus_tag="OO433_003285"
                     /pseudo
     CDS             complement(7598..8578)
                     /locus_tag="OO433_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8702..9694
                     /gene="tehA"
                     /locus_tag="OO433_003286"
     CDS             8702..9694
                     /gene="tehA"
                     /locus_tag="OO433_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6051645.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATMPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9691..>10210
                     /gene="tehB"
                     /locus_tag="OO433_003287"
     CDS             9691..>10210
                     /gene="tehB"
                     /locus_tag="OO433_003287"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6051646.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVG"
BASE COUNT         2598 a         2392 c         2618 g         2602 t
ORIGIN      
        1 taactgaagt gtaaaagggg tgccatgaga aaatgaatct gctagtcaga tgcgcgggga
       61 aaatccccgc gcttgccctt acctggacgt gcaggccatg agcgcagcaa cctccttatc
      121 accgtcccgg aaccgcagtt cgtagagcgt ttgtcgggtc aggagtgtga atgtcagaat
      181 cgtaatgcag ataatgagca gacacaccac cagagggttg tgcttcatag cctttctcct
      241 tgccggatgg cgggtaagag gctaagatct gaattgctag gttcattcgt tggcctcggt
      301 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
      361 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      421 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg attagccagt ttagccattc
      481 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      541 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      601 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      661 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      721 tcgcattaag ttctgctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
      781 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
      841 attcaggccg gggctgcgag tcgtattgcg gaaatggaag caatgaagcg aaatattgcg
      901 caagccgaat cggagattaa acagtcgcag caaggttatc gtgcttatca gaatcgaccg
      961 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt tcaggcttat
     1021 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     1081 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa
     1141 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     1201 cgcctgggtg ggatgttcat gattggcgcg tttgtgcgtg ccctggtcat gacgctgata
     1261 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     1321 gaaaaaatcg ccagtggcga tctgacgatg aatgatgaat cggcgggtcg taatgaaatc
     1381 ggtcgcttaa gtcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     1441 gttcgacagg gcgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg
     1501 gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg
     1561 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     1621 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggagtaaaa acgatgggcg
     1681 ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac agtattgctt
     1741 tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg ggtgagcaag
     1801 ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc agcgctcagg
     1861 cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac ctggggtcgg
     1921 atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc gcgagtgtca
     1981 cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc ataacgcagg
     2041 ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct ctggtagaag
     2101 aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact gaggcggtgg
     2161 atgtattccg tctgaacaaa cattctgtgt cggcagaacc tcgcggagcg ggtgaaccag
     2221 ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct tcacctttca
     2281 gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag agaacatcgc
     2341 atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa caggctgggc
     2401 caccagcgtg gcatgacgca ggtcgtcttt taccgcgcca gaagggacaa accacacgta
     2461 gtcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga tacaacccga
     2521 aggaatttta catccctggc tctgtactaa tgcatctgaa tgctggcgtg gcgcagtgcc
     2581 ttctggtgat acaacgaccg gccatttcag cactcggctt agcgttacgt tctcctgaag
     2641 tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca gttcgtaatt
     2701 aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga tttccccggt
     2761 tttcaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg tcgtctcttt
     2821 ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg cagtaggtag
     2881 tgcaccaatc ctgacgacat cattattaag accttcttta cgatgaagcg actgtccggc
     2941 agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc cgggtaaggt
     3001 aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct gctacagttc
     3061 attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg cgcccccaaa
     3121 gttccttgtt gtgcgacagc tacgaatgta tgaaggtggc gcaaacggat gcgctgacta
     3181 aacagactat ttttttccat aagcgatgtt aaaaacgaag cggtgtcgct gacaagtgaa
     3241 gttgtttgat tatgataact tgattgcaaa atataattaa caattaaagc aattgtgtta
     3301 cagcaaaatg gataatattg atgttttcgc ggcgagatca cagtttgtaa attcttcccg
     3361 ccagagtgaa tgcggttacc tacactccag attactgacc actggaggca gacactatgg
     3421 cgaacagcat cacggcggat gagattcggg aacagttttc gcaggcaatg tcagccatgt
     3481 accagcaaga agttccgcag tacggcccgc tgctggaact ggtagctgat gtgaatctgg
     3541 ctgtgctgga aaacaatcct caactgcacg aaaaaatggt aaatgcagat gagctggcgc
     3601 gactgaatgt tgaacgtcat ggggcgattc gcgttgggac tgcacaagag cttgctactc
     3661 ttcggcggat gtttgccatt atggggatgt acccggtgag ctattacgat ctctcgcagg
     3721 caggggggcc ggtacattcg acagcatttc ggcccattga tgatgcttct ctggcgcgta
     3781 atcccttccg cgtttttacc tccttactcc gccttgagct tatcgagaac gaaattttgc
     3841 gccagaaagc ggcggagatt ctgcgtcagc gcgatatctt caccccacgt tgtcgacaac
     3901 tgttagagga atatgagcag cagggcggtt ttaacgaaac acaggcacag gaatttgtgc
     3961 aagaggccct ggaaacgttt cgctggcacc agtcagcaac ggtagatgaa gaaacctatc
     4021 gcgccttgca caacgaatat cggttgattg ctgatgtggt ctgttttcct ggatgccata
     4081 tcaaccacct gacgccacgt acgctggata ttgaccgggt gcagtcgatg atgcctgaat
     4141 gcggaattga acccaaaatt ctcatcgagg ggccgccgcg ccgcgaggta ccgattttac
     4201 tacgccagac cagctttaaa gcactggaag agacggtgtt gtttgcgggg cagaaacagg
     4261 gcacgcatac cgcgcgcttt ggtgaaattg agcagcgtgg cgtggcatta acgccgaaag
     4321 ggcgacaact gtatgatgat cttctgcgta acgctggaac cgggcaggat aatctcactc
     4381 accaaatgca tttacaggaa accttccgca cttttcctga cagtgagttt ttaatgcgtc
     4441 agcaagggtt ggcatggttc cggtaccgtc tgacgccttc gggtgaggcg catcgtcagg
     4501 cgattcatct tggagacgat ccacagcctt taattgaacg tggttgggta gtggcgcaac
     4561 ccatcaccta tgaagatttc ttgcccgtta gcgcggcggg gatcttccag tcaaatctgg
     4621 gtaatgaaac gcagacacgc agtcacggta atgccagtcg cgaagcattt gagcaggcgt
     4681 tgggttgtcc ggttttggat gagttccagc tttaccagga agcggaagaa cgcagtaaac
     4741 gtcgctgtgg tttgctttaa attctgacca tccgcctttg caaaaatttg cctgatttta
     4801 caaacgaatc aggctcatcc catcgacata aaaaaaatgc cgatttatgc atattctctc
     4861 agttcaacaa ttggattatt aataaatatt gtctagagtg agcggtcata aataagcact
     4921 ttcttgccgc tgaaaacgac cagcgcggga ccattcacaa caccagaagg actcactttc
     4981 aggtatggat cgtagacgat ttattaaagg ttcaatggct atggccgccg tgtgcggtac
     5041 cagcggcatt gcttctcttt tttctcaggc ggcattcgcg gcagattctg atattgccga
     5101 cgggcaaacc cagcgttttg acttctccat tctacagtca atggcgcacg acttagcgca
     5161 aacagcgtgg cgtggtgcgc ctcgtccgtt acctgacacg ctggcgacaa tgacgccgca
     5221 ggcttataac agtattcaat acgacgccga aaaatcgctc tggcataacg ttgagaaccg
     5281 tcaactggac gctcagttct tccatatggg aatgggattc cgtcgccgcg ttcgtatgtt
     5341 ttctgtagat ccagcaacac atctggcgcg tgaaattcac tttcgcccgg agttgttcaa
     5401 atacaacgat gcgggtgttg atacaaaaca attagaaggg caaagcgatc tcggctttgc
     5461 cggttttcgc gtgtttaaag cccccgaact ggcgcgccgt gatgtagtat catttctcgg
     5521 cgcgagttat ttccgcgccg ttgatgatac atatcaatac ggtttgtcgg cccgcggcct
     5581 ggcgatcgac acttacaccg acagtaaaga agagttcccc gactttaccg ccttctggtt
     5641 tgatacggta aaaccggggg caactacctt taccgtttat gcgttgctcg atagcgccag
     5701 cattactggt gcctataagt tcactatcca ttgtgagaaa agtcaggtga ttatggatgt
     5761 ggaaaatcac ctgtatgcgc gcaaagacat taaacagctg ggcattgcgc cgatgaccag
     5821 tatgttcagc tgcggtacta atgaacgtcg gatgtgcgat acaattcatc cgcaaattca
     5881 tgactctgat cgtctgtcca tgtggcgggg caacggcgag tggatttgcc gtccgctgaa
     5941 taatccgcaa aaattgcagt tcaatgctta caccgacaac aacccgaaag ggtttggttt
     6001 attgcaactg gatcgtgact tctcccatta tcaggacatt atgggctggt ataacaaacg
     6061 cccaagtctg tgggtggaac cgcgtaacaa gtggggtaag ggcaccatcg gcctgatgga
     6121 aatcccaaca acgggcgaaa cgctggataa cattgtctgt ttctggcagc cagaaaaagc
     6181 tgtaaaagca ggtgatgagt ttgcattcca gtatcgtctg tactggagtg cgcaaccgcc
     6241 tgttcattgc ccattagcgc gcgttatggc gacgcgtacc ggcatgggcg gtttcccgga
     6301 aggttgggcg ccaggtgaac actatcccga aaaatgggcg cgtcgttttg ccgtcgattt
     6361 cgttggtggt gatctgaaag ctgccgcgcc aaaaggcatt gagccggtga ttacgctttc
     6421 cagtggggaa gcgaagcaaa tcgaaattct ctatattgaa cccatcgatg gttatcgtat
     6481 tcagtttgac tggtatccga cttcggactc cactgatccg gtcgatatgc ggatgtatct
     6541 acgttgtcag ggggacgcta tcagtgaaac atggctgtat cagtatttcc cgccagcgcc
     6601 ggataaacgt cagtatgttg acgaccgcgt gatgagttaa ttgttttttc ttcggcacct
     6661 tcttcgggag gtgccgtctg gttaaacacg atcccgctcg catttttccc taagttaaat
     6721 gagtaatctg atggtgtgta tttcagatac accttgtcag ccactaacag ggagtgcgta
     6781 tgtttccaga ataccgagat ttaatatccc gtctgaaaaa cgaaaatcct cgctttatgt
     6841 ccttgttcga taaacacaat aaacttgatc atgaaattgc cagaaaggaa ggtttcgacg
     6901 gtcgagggta caatgcggaa gtggtccgca tgaaaaaaca aaagctacag ttaaaagatg
     6961 agatgctcaa aatcctgcag caggagagcg tcaaaggggt gtaaactttc ctaagccgcc
     7021 agccaggcgg cttttttaac aacagcatgg attgactgga gataagatga ctgaaacgat
     7081 aaaagtaagc gaatcacttg aattacatgc tgttgcggaa agtcacgtca cacctcttta
     7141 tcagttaatc tgtaaaaata aaacctggtt acagcagtcg ctaaactggc cgcagttcgt
     7201 tcagagtgaa gaggacacgc gaaaaacggt gcagggtaat gtgatgttgc atcaacgcgg
     7261 ctatgccaaa atgttcatga ttttcaaaga agatgaactt atcggcgtta tctcgtttaa
     7321 tcgtattgaa ccactgaata aaactgctga aatcggctac tggctggacg aatctcatca
     7381 ggggcagggg atcatttctc aggcgctgca ggcattgatt catcattacg ctcagtctgg
     7441 tgaacttaga cgcttcgtga tcaaatgtcg ggtggacaat ccgcaaagca accaggtcgc
     7501 tttgcgcaat ggttttatcc ttgaaggttg cctgaaacag gctgagttcc tgaatgatgc
     7561 ctatgatgat gtgaacttat acgcgcgtat tatcgattca taataaccct gccagcggcg
     7621 ttcgcgtaat gcgctcctcg ccgttaatga cttttggacc acggacatga accgtgtcgc
     7681 catcaaaggc ttcaacgacg gcgtggtcgg ttaactccac atggttttca attatcaccg
     7741 cacctgaaat acggctctca ccttgtatta caacgtgctc gtcgagcaga atcggttcac
     7801 cacgtaccac ggcatttccg ccaatcaaga catgatgttt taacacacaa ttaccttcca
     7861 caatggcata ttccgccacc tgcgaactgt aatgaatcgt cggaatggca tcttcttcta
     7921 tgccagcttt cacctgcgcg tgaccgtaga ctttagcgca atcgcatagc cagacgttgt
     7981 tctcttcatt accttcaaca ctggcaaaat caaaaacttc ggcgcgatgt tcaataaaag
     8041 cataccggac gacggcatcg ccataaattt gtgcctggtg gacaatacgc gaggcgctaa
     8101 cccttgcgcg atcataaatt tggagcagga gttgatggtc gggcgttaag ccttgtgcgg
     8161 cgacgatcat agaatgttga tcaattaagg cgtgaccaaa tattctacat tgcccgtaaa
     8221 ctaatgagtc atgaatggtg acactgtcac ttatatatgc accctgactg atttcactat
     8281 tatcgatcca gacgttatcc gttgcgtaaa cctctcccca cagcacgctg gtgccggtga
     8341 tacgcgtatt accagaaatc accgttccgc cgaacgcaat agcattctgg tcgtaaatcc
     8401 agcaatttcc ttcctatgct aacaccgttt cgcgatcgat ccagccgcca gcggttcctg
     8461 ctatcacatc gttaaaatcg ctaatggcga taatctgccg taataacaca ctttttttag
     8521 tgccatcttc ctgataacta aaggctcgct gttcttcact tagacgatat ttgcgcatag
     8581 cgttttctca caggtgactt actataaccg tagcaaattc tgcggctctg gctatgctcg
     8641 agaaaattcc ataaaatgca tttcaaatat actttataaa ttaaacaaaa tgagtaagaa
     8701 gatgcagagc gataaagtgc tcaatttgcc ggcaggctac tttggtattg tgttggggac
     8761 gatagggatg ggatttgcct ggcgctatgc cagccaggtt tggcaggtca gccactggtt
     8821 aggggatggg ctggtgattc tggcgatgat catctgggga ttattaacta gcgcatttat
     8881 tacccgactc atacgctttc cgcatagcgt gctggcggaa gttcgccatc cagtgctgag
     8941 cagttttgtg agtttgtttc cggcaacgac gatgctggtg gcgattggtt ttgttccgtg
     9001 gtttcgccca ctggcggtgt gcctgttcag ttttggtgtc gtggttcagt tggcttatgc
     9061 cgcctggcaa actgcgggat tatggcgcgg atctcaccct gaagaagcta ccatgcctgg
     9121 actgtatctg ccgacagttg ccaacaactt tatcagcgca atggcctgtg gtgcgttggg
     9181 ctacaccgac gccggtctgg tgtttttagg cgcaggcgtt ttctcatggc taagccttga
     9241 accggtgatc ttgcagcgtc tgcgcagttc gggagaatta cccacggcac tgcggacatc
     9301 actcggcatt cagctcgctc ctgcgctggt ggcctgtagt gcctggttga gcgtcaacgg
     9361 cggcgagggt gatacgctgg cgaaaatgct ttttggttat ggactgctgc aactgctgtt
     9421 tatgctacgt ctgatgccat ggtatctctc ccagccattt aatgcttcat tctggagttt
     9481 ctcgttcggc gtatctgcac tggcaaccac cggtttgcat ctggggagtg gcagcgaaaa
     9541 tggatttttc catacgctgg cggtaccgct gtttatcttt accaatttta ttattgcaat
     9601 actgctcatc cgtacttttg cgcttctgat gcagggaaaa ttgttagtca gaaccgagcg
     9661 cgctgtttta atgaaagcag aggacaaaga atgatcattc gtgacgaaaa ctattttact
     9721 gataaatatg aattaacccg cacacactct gaagtactgg aagcggtgaa agtggttaaa
     9781 ccgggtaaaa cgctggatct gggctgtggc aatggtcgta acagtcttta cctggcagcc
     9841 aatggttatg atgttgacgc atgggataaa aatgccatga gtatcgccaa tgtagagcgc
     9901 attaaatcca ttgaaaatct ggataattta cacacccgag tcgttgatct gaataacctc
     9961 acatttgata gacagtacga ttttattctt tcgactgtgg tactgatgtt ccttgaggct
    10021 aaaaccatcc cagggctgat tgccaatatg caacgttgca ctaaacttgg tggttacaac
    10081 ctgattgtgg cggcgatgga taccgctgat tatccatgta ccgtcggctt cccgtttgcc
    10141 ttcaaagagg gagaattacg tcgatattac gaaggctggg agagggtgaa atacaatgaa
    10201 gacgtcggcg
//
LOCUS       DAJTSL010000146        10200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTSL010000146 DAJTSL010000000
VERSION     DAJTSL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.146"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1099)
                     /gene="icd"
                     /locus_tag="OO433_003288"
     CDS             complement(<1..1099)
                     /gene="icd"
                     /locus_tag="OO433_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6051647.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRH"
     gene            1271..1924
                     /gene="rluE"
                     /locus_tag="OO433_003289"
     CDS             1271..1924
                     /gene="rluE"
                     /locus_tag="OO433_003289"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6051648.1"
                     /translation="MRQFIISENTMQKTSFRNHLVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            1934..2395
                     /gene="nudJ"
                     /locus_tag="OO433_003290"
     CDS             1934..2395
                     /gene="nudJ"
                     /locus_tag="OO433_003290"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6051649.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2449..3555
                     /gene="mnmA"
                     /locus_tag="OO433_003291"
     CDS             2449..3555
                     /gene="mnmA"
                     /locus_tag="OO433_003291"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6051650.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3591..4232
                     /gene="hflD"
                     /locus_tag="OO433_003292"
     CDS             3591..4232
                     /gene="hflD"
                     /locus_tag="OO433_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6051651.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVPAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4236..5606
                     /gene="purB"
                     /locus_tag="OO433_003293"
     CDS             4236..5606
                     /gene="purB"
                     /locus_tag="OO433_003293"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6051652.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5774..6445
                     /gene="phoP"
                     /locus_tag="OO433_003294"
     CDS             5774..6445
                     /gene="phoP"
                     /locus_tag="OO433_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6051653.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6445..7905
                     /gene="phoQ"
                     /locus_tag="OO433_003295"
     CDS             6445..7905
                     /gene="phoQ"
                     /locus_tag="OO433_003295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6051654.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            7981..9102
                     /gene="roxA"
                     /locus_tag="OO433_003296"
                     /gene_synonym="ycfD"
     CDS             7981..9102
                     /gene="roxA"
                     /locus_tag="OO433_003296"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6051655.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9151..>10200)
                     /gene="pepT"
                     /locus_tag="OO433_003297"
     CDS             complement(9151..>10200)
                     /gene="pepT"
                     /locus_tag="OO433_003297"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6051656.1"
                     /translation="ATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGDI
                     ALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNIP
                     HGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIVG
                     NNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLAGMKGTVERADMHY
                     IIRDFDRKQFEARKCKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILDI
                     AQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEKA
                     VQVIVRIAELTAQRK"
BASE COUNT         2471 a         2629 c         2616 g         2484 t
ORIGIN      
        1 tatggcgcag catcatctca gcggagagga tgatagagcc tggatttact ttgtcctgac
       61 cggcgtattt cggcgcagta ccgtgggtgg cttcaaacag ggcgcattcg tcaccgatgt
      121 ttgctccagg agcgataccg ataccgccaa cctgcgctgc cagggcgtca gaaatgtagt
      181 caccgttcag gttcatacag gcgataacat catattcagc cggacgcagc aggatctgtt
      241 gcaggaatgc atcagcaatc acgtctttaa tgacgatctc tttgccggtg ttcgggtttt
      301 taactttcag ccacgggccg ccgtcgatca gttcaccgcc aaactcttca cgcgccagct
      361 ggtagcccca gtctttaaac gcgccttcgg tgaacttcat gatgttgcct ttgtgcacca
      421 gagtcacaga gtcacgatcg ttagcaattg cgtattcgat cgctgcacga accagacgtt
      481 tggtgccttc ttccgaacac ggcttaatac cgataccaca atgttccggg aagcgaattt
      541 tcttcacccc catctcttca cgcaggaatt taatcacttt ctcggcgtcg gcagagtctg
      601 ctttccattc gatacccgca taaatgtctt ccgagttttc acggaagata accatatcgg
      661 tcagttcagg gtgtttaacc gggcttggag tgccctgata gtaacgtacc ggacgcaggc
      721 agatgtagag atccagttcc tggcgcaggg caacgttcag agagcgaata ccgccaccaa
      781 caggagtggt cagcggacct ttaatggcaa cgcgatattc acgaatcaga tcaagggttt
      841 cagcaggcag ccagacatcc tgaccataaa cctgtgtgga tttttcaccg gtgtaaattt
      901 ccatccagga gattttacgc tcgcctttat aggctttctc gactgcagcg tcgaccactt
      961 tcagcatggc tggggttaca tctacaccga ttccatcacc ttcaatgtaa gggataatcg
     1021 gattttcagg aacgttgagt ttgccgtttt gcagggtgat cttcttgcct tgtgccggaa
     1081 caactacttt actttccatt cacctctcct tcgagcgcta ctggtttgct cgtctgccac
     1141 cacgttgcat atgcgtttgc gtcctgcgat acggatgctt tagagcaatt ttttgttaat
     1201 gatttgtaat tggcttgtca atactaccct attgtttgcc gtcatgaaag atgcgcgtta
     1261 ttaggctata atgcggcaat tcataatctc tgaaaatacc atgcaaaaaa cttcttttag
     1321 aaatcacctg gttaagcgat tcagctcgca acgttctacc aggcgtaaac ctgaaaacca
     1381 gcccacgcgt gtgatcctgt tcaataaacc ctacgatgtt cttccgcagt tcaccgatga
     1441 agccggacgc aaaacattaa aagaattcat cccggttcag ggcgtttatg cagcaggtcg
     1501 tcttgaccgc gatagcgaag ggttactggt gctgaccaat aacggcgcgt tgcaggcgcg
     1561 tttaacccag ccgggcaaac gcaccgggaa aatctattat gtgcaggtgg aaggtattcc
     1621 cacacaagac gcacttgaag ccttgcgcaa tggcgtaacc ttaaatgatg gccctaccct
     1681 gcccgccggc gcggagctgg ttgacgaacc agcgtggtta tggccgcgga atccaccaat
     1741 tcgtgaacgc aaaagtattc ccaccagctg gctgaagatc accttatatg aaggacgtaa
     1801 tcgccaggtg cgccgcatga ccgcccatgt tggtttcccc acgctgcgac tgattcgtta
     1861 tgcgatgggt gattacactt tggataacct tgccaatggc gaatggcgag aagcaacaga
     1921 ataaggatgc acaatgttta aaccacacgt taccgttgct tgcgtagtgc acgcagaggg
     1981 taaattttta gtcgttgaag agacgattaa tggtaaagcg ttatggaacc aacctgccgg
     2041 gcatctggaa gccgatgaaa ccttagtgga agctgccgcc cgtgagctat gggaagaaac
     2101 cggcatcagc gcgcaaccgc aacactttat tcgtatgcat cagtggattg cgccagataa
     2161 aacgccgttt ttgcgtttcc tctttgccat tgagcttgag caaatatgcc cgacccagcc
     2221 tcatgacagc gatatcgact gctgtcgttg ggtcagcgcc gaagaaattt taaaggcgtc
     2281 aaatcttcgt tcgccgctgg tggcggaaag tatccgttgt tatcaaagcg ggcaacgcta
     2341 tccgctggag atgattggcg attttaactg gccttttaca aagggtgtca tctaaaaggc
     2401 tgcgtgatag aatacgccgc cttgaagttc aatgtcgtga gtgatccaat gtctgaaacc
     2461 gcaaaaaaag taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc tgcctggctg
     2521 ttgcaacaac agggatatca ggttgaaggc ctgtttatga agaactggga agaagacgac
     2581 ggtgaggaat attgcacagc ggcagcggat ctggctgatg cccaagctgt ctgcgacaag
     2641 ctcggcattg aactgcacac cgttaacttt gctgccgagt actgggacaa cgtcttcgaa
     2701 ctgttccttg ccgaatataa agccggtcgc acgccgaatc cggatattct gtgcaacaaa
     2761 gagatcaaat ttaaagcttt cctcgaattt gccgccgaag atttaggtgc cgattatatc
     2821 gctaccggtc attacgtacg tcgggccgat gtcgatggca agagccgcct gctgcgtggt
     2881 ctggacagca ataaagacca gagctacttc ctttatacgc tcagccatga gcagattgcg
     2941 caaagcctgt tcccggtcgg cgaactggaa aaaccgcagg tgcgtaagat tgctgaagat
     3001 cttggtctgg tcaccgcgaa gaaaaaagac tctaccggca tttgcttcat tggcgaacgt
     3061 aaattccgcg agttcctggg ccgttatctc ccggcgcaac cgggcaaaat cattaccgtc
     3121 gatggcgatg aaattggtga gcaccagggg ctgatgtatc acactctcgg tcagcgtaaa
     3181 ggtctgggta tcggtggcac caaagaaggc accgaagaac cgtggtatgt ggtggacaaa
     3241 gacgtcgaaa acaacattct ggttgttgcg cagggccatg aacacccgcg gctgatgtct
     3301 gtcgggttga ttgcccagca gttgcactgg gtcgatcgcg aaccattcac cggtactatg
     3361 cgttgcacgg taaaaacccg ctatcgccag accgatatcc cttgcaccgt caaggcgctg
     3421 gacgatgatc gcattgaagt gattttcgat gaaccggttg ccgccgtgac gccgggccag
     3481 tctgccgtct tctataacgg tgaagtgtgc ctcggtggcg gtattattga gcagcgtctg
     3541 ccgctgccgg tctgattatt atctttactt aaaacaagga agcagtgaac gtggcaaaga
     3601 attactatga catcaccctc gccctggccg gtatttgtca gtcggcacgc ctggtgcaac
     3661 aactcgctca ccaggggcat tgtgatgccg atgcgctaca cgtctcactc aacagtatta
     3721 ttgatatgaa ccccagctcg acgctggcgg tttttggcgg tagcgaagcc aacctgcgcg
     3781 tcgggctgga aaccctgctc ggcgtgctca atgccagcag tcgccagggc ttaaacgccg
     3841 aattaacccg ctacacactc agcttgatgg tgcttgagcg caaactctcc tcagcgaaag
     3901 gcgcgctcga cactctgggc aaccggatca acggcctgca acgccagctc gaacacttcg
     3961 atttacagtc cgaaacgctg atgagcgcga tggctgctat ctatgttgat gtgatcagcc
     4021 cgcttggccc gcgcattcag gtcaccggtt cccctgctgt actgcaaagc ccacaagtgc
     4081 cggcgaaagt tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc tggcaccagg
     4141 tcggcggcgg acgtctgcaa ctgatgtttt ctcgtaatcg cctgaccact caggcaaaac
     4201 aaattcttgc tcatttaacc ccggagttgt gatctatgga attatcctca ctgaccgccg
     4261 tttcccctgt cgatggacgc tacggcgata aagtcagcgc gctgcgcggg attttcagcg
     4321 aatatggttt gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa aaactggccg
     4381 cgcacgcagc gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt taccttgatg
     4441 caattgtcgc caatttcagc gaagaagatg ccgcacgcat caaaaccatc gagcgtacca
     4501 ctaaccacga cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg gagatcccgg
     4561 aactgcacgc ggtttctgaa ttcatccact ttgcctgtac ttcggaagat atcaataacc
     4621 tctcccacgc attaatgctg aaaaccgcgc gtgatgaagt gatcctgccg tactggcgtc
     4681 aactgattga tggcattaaa gatctcgccg ttcagtaccg cgatatcccg ctgctgtctc
     4741 gtacccacgg tcagccagcc acgccgtcaa ccatcggtaa agagatggca aacgtcgcct
     4801 accgtatgga gcgccagtac cgccagctta accaggtgga gatcctcggc aaaatcaacg
     4861 gcgcggtcgg taactataac gcccacatcg ccgcttaccc ggaagttgac tggcatcagt
     4921 tcagcgaaga gttcgtcacc tcgctgggta ttcagtggaa cccgtacact acccagattg
     4981 aaccgcacga ctacattgcc gaactgtttg attgcgttgc gcgcttcaac accattctga
     5041 tcgactttga ccgtgacgtc tggggttata tcgcccttaa ccacttcaaa cagaaaacca
     5101 ttgctggtga gattggttct tccaccatgc cgcataaagt taacccgatc gacttcgaga
     5161 actccgaagg gaatctgggc ctttccaacg cggtattaca gcatctggca agcaaactgc
     5221 cggtttcccg ctggcagcgt gacctgaccg actccaccgt gctgcgtaac ctcggcgtgg
     5281 gtatcggtta tgcgctgatt gcgtatcaat ccaccctgaa aggcgtgagc aaactggaag
     5341 tgaaccgtga ccatctgctg gatgagctgg atcacaactg ggaagtgctg gctgagccaa
     5401 tccagacagt tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg aaagagctga
     5461 ctcgtggtaa gcgcgttgac gccgaaggca tgaagcagtt tatcgacggt ctggcgttgc
     5521 cagaagaaga gaaagcccgc ctgaaagcga tgacgccggc aaactacatt ggtcgcgcca
     5581 tcaccatggt tgatgagctg aaataaactc gtatcagtgc cggatggcga tgctgtccgg
     5641 cctgcttatt aagattatcc gctttttatt ttttcacttt acctcccctc cccgctggtt
     5701 tatttaatgt ttacccccat aaccacataa tcgcgttaca ctattttaat aattaagaca
     5761 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     5821 taaagttcag attcaggatg ctggtcatca ggtcgatgat gcagaagatg ccaaagaagc
     5881 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgccaga
     5941 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaat gatgtttcac tgccgattct
     6001 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ctggtgctga
     6061 tgattacgtg actaaaccat ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6121 gcggcgtaat agcggtctgg cttcacaggt catttcgcta cctccgtttc aggttgatct
     6181 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6241 cattatggaa acgttgatac gcaataatgg caaagtggtg agcaaagatt cgttaatgct
     6301 ccaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6361 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtg attactaccg ttcgcggcca
     6421 gggctatttg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6481 ctgcgggtac gttttctgtt ggcaacggct gcggtagtac tggtgctttc gcttgcctac
     6541 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6601 cgtggcgaga gcaatctgtt ctataccctt gcgaagtggg aaaacaataa gttgcatgtc
     6661 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6721 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaagatgat ccagcctgac
     6781 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     6841 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     6901 gcggagatga cccactcggt ggcagtaaac gtctacccgg caacatcgcg gatgccaaaa
     6961 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7021 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7081 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7141 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7201 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7261 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7321 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7381 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7441 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7501 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7561 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7621 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7681 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7741 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     7801 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     7861 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccatacttc
     7921 acgcattacg ttaagcatcc gttataatcg gttgcagata ccagcctgtg gatgcttaac
     7981 atggaatacc aactcactct taactggccc gattttcttg aacgtcactg gcagaaacgc
     8041 ccggtggtgt taaaacgcgg ctttaataat tttattgacc cgatctctcc agacgagttg
     8101 gcgggtctgg cgatggaaag cgaagttgac agtcgactgg tcagtcacca ggatggcaaa
     8161 tggcaggtca gccacggccc gttcgaaagc tacgatcatc tcggtgaaac caactggtca
     8221 ttactggtac aggcagtgaa ccactggcat gagccgaccg ccgcgctgat gcgaccgttc
     8281 cgtgaactac cggactggcg tattgatgat ctgatgattt ctttttctgt acctggcggc
     8341 ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca ttcagggtac cggacgtcgt
     8401 cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact gcccacatcc ggatctgtta
     8461 caggtcgatc cgttcgaagc catcatcgat gaagagctgg agcctggtga tattctttat
     8521 attccgccag gattcccgca tgaaggctac gcgctggaaa atgcgatgaa ctattccgtg
     8581 ggctttcgcg cgccaaatac gcgggaactg attagtggat ttgccgatta tgtgctgcaa
     8641 cgtgaactgg gcggcaacta ctacagcgat cctgatgttc cacctcgcgc tcatcctgcg
     8701 gatgttctgc ctcaagagat ggataaactg cgtgagatga tgctcgaatt gatcaaccag
     8761 ccggaacact ttaagcaatg gtttggcgag tttatatccc agtcacgtca tgaactggat
     8821 atcgcgccgc cggaaccgcc ttatcagcca gatgaaatct acgatgcgct gaaacaaggt
     8881 gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca ttggcgacga cgtgtatgcc
     8941 aatggtgaga agatcgattc cccgcaccgt ccggcactgg atgcactcgc cagcaacatt
     9001 gcgctgactg cggagaattt tggcgatgcg ctggaagatc cgtcattcct cgcgatgctc
     9061 gcggcgctgg tcaatagcgg gtattggttc ttcgaagggt aagtttggat ttaagccgga
     9121 tgcggcatca catgccgcat cccttttcgc ttacttccgt tgcgccgtta actcggcaat
     9181 acggacgatc acctgcaccg ctttttccat accttccaga gtcacaaact catgcttacc
     9241 atgatagttg taaccgccag tgaacaagtt cgggcacggt aatcccataa acgacaactg
     9301 cgcgccgtcg gtaccgccgc ggatcggttt cagttccggt tcaatatcgc agtcacgcat
     9361 tgcctgctgg gcgatatcga gaatatgcgg atgctcaacc actttctcgc gcatattgta
     9421 gtaactgtct tcaatcacca gttcaatgta gcaatcagga tgtaaccctt tgcccacttt
     9481 tttggcgatc tccatcattt tacatttacg cgcttcaaac tgtttacggt cgaaatcacg
     9541 gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc atgcccgcca ggtgatagaa
     9601 accttcatag ccttctgtca tttccgggct ttcatccgcc ggaacttccg catgaatacg
     9661 tgccgccagc gacagcgcat ttaccatcac tcctttcgcc gtgcccggat gaacattgtt
     9721 accgacaatt ttgatattga ccgatgcggc gttgaagttt tcaaactcca gttcgcctac
     9781 gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag gcatcaacat caaaatgttt
     9841 cgcccctttg cccacttctt catccggggt aaaggcgacg cgaatatcac catgcggaat
     9901 gtttttctgt tgcaataccg ccagcgcggt catgatttct gcaatacctg ctttgtcatc
     9961 ggcacctaac aaggttttac catcggtggt aatcaatgtc tgacccagta gctgatgcag
    10021 caccgggaac ataaccggtg ataaaacttc atcgccgata cccagcgcaa tatcgccacc
    10081 gcgatagttt tcaacaattt gcggattaac atttttgccg ctgcaatccg gtgaggtatc
    10141 cacatgagaa ataaagccaa tcgccgggat atcgccaggg acgttagccg gtaacgtcgc
//
LOCUS       DAJTSL010000147        10156 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTSL010000147 DAJTSL010000000
VERSION     DAJTSL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.147"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..614
                     /locus_tag="OO433_003298"
     CDS             <1..614
                     /locus_tag="OO433_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6051657.1"
                     /translation="SILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQMIA
                     LRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDYSW
                     IVQPVANEKTLHSLAHGAGRKWGRTECKWRLAAKYTVTQLSRTELGSRIICRDKQLIF
                     EEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            611..1225
                     /locus_tag="OO433_003299"
     CDS             611..1225
                     /locus_tag="OO433_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR6051658.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1282..2739)
                     /gene="pepD"
                     /locus_tag="OO433_003300"
     CDS             complement(1282..2739)
                     /gene="pepD"
                     /locus_tag="OO433_003300"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6051659.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3000..3458
                     /gene="gpt"
                     /locus_tag="OO433_003301"
     CDS             3000..3458
                     /gene="gpt"
                     /locus_tag="OO433_003301"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6051660.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3550..4794
                     /gene="frsA"
                     /locus_tag="OO433_003302"
     CDS             3550..4794
                     /gene="frsA"
                     /locus_tag="OO433_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6051661.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4852..5253
                     /gene="crl"
                     /locus_tag="OO433_003303"
     CDS             4852..5253
                     /gene="crl"
                     /locus_tag="OO433_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6051662.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5292..6347)
                     /gene="phoE"
                     /locus_tag="OO433_003304"
     CDS             complement(5292..6347)
                     /gene="phoE"
                     /locus_tag="OO433_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6051663.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6635..7738
                     /gene="proB"
                     /locus_tag="OO433_003305"
     CDS             6635..7738
                     /gene="proB"
                     /locus_tag="OO433_003305"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6051664.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7750..9003
                     /gene="proA"
                     /locus_tag="OO433_003306"
     CDS             7750..9003
                     /gene="proA"
                     /locus_tag="OO433_003306"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6051665.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9118..9193
                     /locus_tag="OO433_003307"
     tRNA            9118..9193
                     /locus_tag="OO433_003307"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9151..9153,aa:Thr,seq:cgt)
     gene            complement(9208..>10156)
                     /locus_tag="OO433_003308"
     CDS             complement(9208..>10156)
                     /locus_tag="OO433_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6051666.1"
                     /translation="KKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHE
                     ENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIK
                     VPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRR
                     VAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRK
                     MRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRR
                     YAHLAPNHLTEHARQIDSILNPSVPNLSQSRNKERTNDV"
BASE COUNT         2432 a         2500 c         2645 g         2579 t
ORIGIN      
        1 agtctatttt acagcggcat attgcctcgt tctcgcatca tggtttgcct caaggcagtg
       61 acgacgcgct acggtatatt gcggaacatg acgatgcgct ggcgtttgcg tgtattaatc
      121 gccagatgat cgctttgcgc ataatgcaac aagttaaggc cacgggaagt ccggttctgg
      181 atgtggcgca taactttgtt agcgcgtgtc aaatcggcga tcaacagggc tggttgcatc
      241 gtaaaggtgc cacgccggat gacaacggtc tggtgattat tcccggttca cgtggtgatt
      301 actcctggat tgtacagcct gttgcgaacg aaaaaacgct ccactcgctg gcgcatgggg
      361 cagggcgtaa atgggggcgc accgagtgta aatggcgtct ggcagcgaaa tacacggtga
      421 cgcaactctc gcggactgaa cttggcagtc ggataatttg tcgcgataaa caactcatct
      481 ttgaagaagc gccacaagcc tataaatcgg ctgaaagcgt ggtgcaatgt ctggtgcagg
      541 ccggtttaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa aacagtggag
      601 ggaaaaaagg atgatcttgc tacaactctc ctcagcgcag gggccggaag aatgttgtct
      661 cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag acgtcgcggt
      721 aacggtgttg gaaacagaaa cgggccgcta ctctgacacg ctgcgttcag ctctggtttc
      781 tctggatggc gataacgcat gggcattaag cgaaagctgg tgtggcacta ttcagtggat
      841 ttgtccgagt ccgtatcggc ctcatcatgg gcgcaaaaac tggtttctgg gcattgggcg
      901 ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc tgcgttcgtc
      961 ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca cgcatttggc
     1021 atccggtatt agcgtgaagg ttcagtcaga gcgtagtcag catgctaaca agcggctggc
     1081 acgattgctg attgcctgga agctggagca gcagcaacag gaaaatagcg cggtgctgaa
     1141 atcgcagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgagac ggacatttac
     1201 agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg attaaacgcc
     1261 atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt cagtcagcag
     1321 tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt gtggaccggt
     1381 gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga acagaccaca
     1441 ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc gctgataggt
     1501 ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag gatatgcgcc
     1561 tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc tcaccacgta
     1621 gtctttaccg ctgtcgatca gtgaacggat caggcagtga atttctacgt tattgtcagt
     1681 catggtcacc acaccgacgt tcagggaggt ttcaaccaca cctttggcta catcggagtt
     1741 gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc gagatttcgc
     1801 aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat ttttctcttt
     1861 ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt tcagtgcgtc
     1921 gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg cgttacgcag
     1981 tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac ccgccaggaa
     2041 gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc cggagtgacc
     2101 gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa ccgcttcacg
     2161 atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt agatttcacc
     2221 ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc tctgtaagcc
     2281 aaaagcaccg tccataccgg cttcttcggt catggtcagc agcacttcca gcggaccgtg
     2341 aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc cgttatccgc
     2401 acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct ggataggatc
     2461 tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga ggtgggcctg
     2521 taagacgacc ggtttacgat tttccatacc tgcggtagca ggtttacgaa tcaggatatt
     2581 acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa caatgtattc
     2641 agcgagttgc tcttcatgat aggacgggtg cggaatagaa cagattttgg caaaaatatc
     2701 ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc ttgtcgatca
     2761 cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac acaagacagg
     2821 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     2881 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     2941 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     3001 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     3061 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgg
     3121 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
     3181 ccagctacga tcacgacaac cagcgagagc ttaaagtgct gaaacgcgca gaaggcgatg
     3241 gcgaaggctt catagttatt gatgacctgg tggataccgg tggtactgct gttgcgattc
     3301 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
     3361 tggttgatga ctatgttgtt gatattccgc aagatacctg gatcgaacag ccgtgggata
     3421 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
     3481 cgggcgttgt tctttttaac ttcaggcagg ttacaatagt ttccagtaag tattctggag
     3541 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
     3601 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
     3661 gctcttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
     3721 tggcgcggta ttgacccacg tgaaatcctc gacgtccagg cacgtattgt gatgagcgat
     3781 gctgaacgta ccgacgatga tttatacgat actgtgattg gctaccgtgg tggcaactgg
     3841 atttatgagt gggccaccca ggcgatggtg tggcaacaaa aagcctgtgc ggaagaagat
     3901 ccgcaactca gtggtcgaca ctggctgcat gcggctacgt tgtacaacat tgccgcctat
     3961 cctcatctga aaggagatga cctggccgag caagcgcagg ctttatcaaa ccgcgcctat
     4021 gaagaggccg cccagcgtct gccgggcacg atgcggcaga tggagtttac cgtacccggc
     4081 ggtgcgccca tcaccggctt tttgcatatg ccgaaaggcg atggcccgtt cccgacagta
     4141 ttaatgtgtg gtggtctgga tgcgatgcag acggactatt acagcctgta tgaacgttat
     4201 tttgcgccgc gcggcattgt gatgctgact attgatatgc cgtcggtggg cttttcttca
     4261 aaatggaagc tcacccagga ctccagcctg ttgcatcagc acgtcttaaa ggcgctgcct
     4321 aacgtaccgt gggtggatca cactcgcgtc gcggcctttg gtttccgttt cggcgctaac
     4381 gttgccgtgc gtctggcata tcttgaatcg ccgcgtctga aagcggttgc ctgtcttggt
     4441 ccggtagttc ataccctgtt gagtgatttt aagtgccagc aacaggtgcc ggaaatgtat
     4501 cttgacgttc tggcgagtcg tttggggatg catgatgctt ccgacgaagc gttacgcgtg
     4561 gagctgaatc gctattcatt aaaagtgcaa ggattgctgg gacgtcgctg cccaacgcca
     4621 atgttatcag gctactggaa gaacgatccg ttcagcccgg aagaggactc acgcttaatc
     4681 acctcatcat ctgctgacgg taaattatta gagatcccat ttaacccggt gtatcggaat
     4741 tttgacaaag gtcttcagga aatcaccggc tggatcgaaa aacgcttgtg ttaaaaattt
     4801 gctaaatttt gccaatttgg taaaacagtt gcatcacaac aggagatagc aatgacgtta
     4861 ccgagtggac acccgaagag cagattgatc aaaaaattta ccgcactagg cccgtatatt
     4921 cgtgaaggta agtgcgaaga taatcgattc tttttcgatt gtctggctgt atgcgtcaac
     4981 gtgaaaccgg caccggaagt gcgtgaattc tggggctggt ggatggagct ggaagcgcag
     5041 gaatcccgtt ttacctacag ctaccagttt ggtctgttcg ataaagcagg cgactggaag
     5101 agtgttccgg taaaagatac tgaagtggtt gaacgactgg agcacaccct gcgtgagttt
     5161 cacgagaagc tgcgtgaact gctgacgacg ctgaatctga agctggaacc ggcggatgat
     5221 tttcgtgacg agccggtgaa gttaacggcg tgagtgaaat gtgccggatg catcacatcc
     5281 ggcaatatct attaaaactg atacgtcatc ccaatcgcga caatatcatc attattaata
     5341 ttcaatttgt tatcgctatc cagttggttg attttataat caacaaatgc tgacatattt
     5401 ttgttgaaat aatacgtagc accgacgtcg atataattaa ccagatcttc accaccgatg
     5461 ccctcaatat ctttcccttt cgataagaca taacccagcg atggacgcag accaaagtca
     5521 aactggtatt gagcgaccgc ttcaaagttc tgtgtcttat tggcaaagcc gccagttatt
     5581 ggcgtcattt tgcgagtttc agaatagaaa gttgccagat aaatattatt ggcatcgtat
     5641 ttcagacctg ttgcccatgc ttctgcacgc ttgcctgtgc cacggctttg caggttctgc
     5701 tcgttggtgc gatctgagtt ggtataggcc ccactaatgg cgaaatcgct gccgccaaag
     5761 tcatatgtca atgacgtgcc gaagccatcg ccgttttgct ttttaacgtc gcggttttcg
     5821 tttttccctt gatattgcag ggttaagttc aggccatcga taacgccgaa gaagtcggtg
     5881 ttccgatacg tcgccagacc gctggcgcgt ttggtcataa agttgtcggt ctgcgccgag
     5941 gagtcgccac caaattccgg aaacatatcg gtccaggctt ccacgtcata caacgcgccc
     6001 aggttacgac cataatcgaa agaacccaaa tctttatatt tcaacccggc aaaagcgaga
     6061 cgcgtttttt gctgtgcagt atcactctct gctttattac cggcaaactc tgcttcccaa
     6121 cgaccataac cagtcagttg atcgttaatt tgtgtttcgc ctttaaaacc aaaacggata
     6181 taactctggt cgccatcttt actgtcgtta tcactcatat aatgcatggc tttaactttg
     6241 ccatagacat ccagtttatt accgtctttg ttatatattt ctgcggcctg tacggatgca
     6301 gatgccacaa tgcccatcac cactaatgcc agagtgctct ttttcatttt cattcctgat
     6361 tttaattaac gcgcgaatat tcagcgggag agccccgttg aaaacaggaa agtttttaac
     6421 ctgagattgt taaagatata ttacagatta ataatattct taaaatgtgg taatttatta
     6481 aatctgtaat aaaagcgtaa acaactgccg ctaggcttgc tgatcccgcg caacaaaacg
     6541 ccatgctttg ctcgcagatg gttggcaacc gacgacagtc ctgctaaaac gttcgtttga
     6601 tatcattttt cctaaaattg aatggcagag aatcatgagt gacagccaga cgctggtggt
     6661 aaaactcggc accagtgtgc taacaggcgg atcgcgccgt ctgaaccgtg cccatatcgt
     6721 tgaacttgtt cgccagtgcg cgcagttaca tgccgccggg catcggattg ttattgtgac
     6781 gtcgggcgcg atcgccgccg gacgtgagca cttgggttac ccggaactgc cagcgactat
     6841 cgcctcgaaa caactgctgg cagcggtagg gcagagtcga ctgattcaac tgtgggaaca
     6901 gctgttttcg atttatggca ttcacgtcgg gcaaatgctg ctgacccgtg ctgatatgga
     6961 agaccgtgaa cgcttcctga acgcccgcga caccctgcgt gcgttgctcg ataacaatat
     7021 cgttccggta atcaatgaga acgatgctgt cgctacggca gagattaaag tcggcgataa
     7081 cgataacctt tctgcgctgg cggcgattct ggcgggtgcc gataaactgt tgctgctgac
     7141 cgatcaaaaa ggtttgtata ccgctgaccc gcgcagcaat ccgcaggcag aactgattaa
     7201 agatgtttac ggcattgatg acgcactgcg cgcgattgcc ggtgacagcg tttcaggcct
     7261 cggaactggc ggcatgagta ccaaattgca ggccgctgac gtggcttgcc gtgcgggtat
     7321 cgacaccatt attgccgcgg gcagcaagcc gggcgttatt ggtgatgtga tggaaggcat
     7381 ttccgtcggt acgctgttcc atgcccaggc gactccgctt gaaaaccgta aacgctggat
     7441 tttcggtgcg ccgccggcgg gtgaaatcac ggtagatgaa ggggcaactg ccgccattct
     7501 ggaacgcggc agctccctgt tgccgaaagg cattaaaagc gtgactggca atttctcgcg
     7561 tggtgaagtc atccgcattt gcaacctcga aggtcgcgat atcgcccacg gtgtcagtcg
     7621 ttacaacagc gatgcattac gccgtattgc cggacaccac tcgcaagaaa ttgatgcaat
     7681 actgggatat gaatacggcc cggttgccgt tcaccgtgat gacatgatta cccgttaagg
     7741 agctggccga tgctggaaca aatgggcatt gccgcgaagc aagcctcgta taaattagcg
     7801 caactctcca gccgcgaaaa aaatcgcgtg ctggaaaaaa tcgccgatga actggaagca
     7861 caaagcgaaa tcattctcaa cgctaacgcc caggatgttg ctgacgcgcg agccaatggc
     7921 cttagcgaag cgatgcttga ccgtctggca ctgacgcccg cacggctgaa aggcattgcc
     7981 gacgatgtac gtcaggtgtg caacctcgcc gatccggtgg ggcaggtaat cgatggcggc
     8041 gtactggaca gcggcctgcg tcttgagcgt cgtcgcgtac cgctgggagt tattggcgtg
     8101 atttatgaag cgcgcccgaa cgtgacggtt gatgtcgctt cgctgtgcct gaaaaccggt
     8161 aatgcggtga tcctgcgcgg tggcaaagaa acgtgtcgca ctaacgctgc aacagtggcg
     8221 gtgattcagg acgccctgaa atcctgcggc ttaccggcgg gtgccgtgca ggcgattgat
     8281 aatcctgacc gtgcgctggt cagtgaaatg ctgcgtatgg ataaatacat cgacatgctg
     8341 atcccgcgtg gtggcgctgg tttgcataaa ctgtgccgtg aacagtcgac aatcccggtg
     8401 atcacaggtg gtataggcgt atgccatatt tacgttgatg aaagtgtaga gatcgctgaa
     8461 gcattaaaag tgatcgtcaa cgcgaaaact cagcgtccga gcacatgtaa tacggttgaa
     8521 acgttgctgg tgaataaaaa catcgccgat agcttcctgc ccgcattaag caaacaaatg
     8581 gcggaaagcg gcgtgacatt acacgcagat gcagctgcac tggcgcagtt gcaggcaggc
     8641 cctgcgaagg tggttgctgt taaagccgaa gagtatgacg atgagtttct gtcattagat
     8701 ttgaacgtca aaatcgtcag cgatcttgac gatgccatcg cccatattcg tgaacacggc
     8761 acacaacact ccgatgcgat cctgacctgc gatatgcgca acgcccagcg ttttgttaac
     8821 gaagtggatt cgtccgctgt ttacgttaac gcctctacgc gttttaccga cggcggccag
     8881 tttggtctgg gtgcggaagt ggcggtaagc acacaaaaac tccacgcgcg tggcccaatg
     8941 gggctggaag cactgaccac ttacaagtgg atcggcattg gtgattacac cattcgtgcg
     9001 taaataaaac cgggtgatgc aaaagtagcc atttgattca caaggccatt gacgcatcgc
     9061 ccggttagtt ttaaccttgt ccaccgtgat tcacgttcgt gaacatgtcc tttcagggcc
     9121 gatatagctc agttggtaga gcagcgcatt cgtaatgcga aggtcgtagg ttcgactcct
     9181 attatcggca ccatttaaat caataagtta cacatcatta gtacgttcct tatttcttga
     9241 ctgggacaaa tttgggaccg atgggttcag aatcgagtct atttgccgtg cgtgttcggt
     9301 aaggtgatta ggtgcgaggt gagcatatcg acgaaccatt tcgatagact cccagcctcc
     9361 catttcctgt aacactgata acgggactcc ggcttgaacc agccaacttg cccaggtgtg
     9421 tctcaagtcg tgaaatctga aatcatcaat acctgctcgt ctcagcgccg ctttccaggc
     9481 tgtgtttgcg tcataccgca tcttcctgac tgttggcgct ttcgttccgt ctggtttggt
     9541 acagctttcc ttatacacaa atacccaacg gtgatgattc ccgatttgtt ttttcaatac
     9601 gcgacatgca gtatcattca gcgcaacgcc aattgcgcgg tttgatttac tctcttccgg
     9661 gtttatccat gccacccggc gctgcatgtc tatttgttgc cattcaaggt tgatgatgtt
     9721 cgagcgtctt aagcctgttg caagtgcaaa ttcaacaaca gactttaatg gctccggaca
     9781 ttcatcaatc agcctttgtg cttcatgagg ctccagccag cggatccgtt tattctttgg
     9841 ttgaggcact ttaataattg gtgccttatc cagcattttc cattcacgct ctgcggctct
     9901 tagcagggcc tttataaatg aaagatgcgt agccttcgtt gcaacggacg ctggttttgg
     9961 cgtgtattct ggaacaggtt tccctttttt tctgcatgct tctgccctga gtttccagtt
    10021 ttcctcatga cgccggttcg tcattttctg cattgctgaa taaatttttg attcagtaat
    10081 gtctcttagt tgcattcctg cgaaatgttg aagccagaat ccgatccggc ttttgtcatc
    10141 gtccagtgat ttttta
//
LOCUS       DAJTSL010000148        10117 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTSL010000148 DAJTSL010000000
VERSION     DAJTSL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.148"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            205..357
                     /gene="hokA"
                     /locus_tag="OO433_003309"
     CDS             205..357
                     /gene="hokA"
                     /locus_tag="OO433_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6051667.1"
                     /translation="MPQKYGLLSLIVICFTLLFFTWMVRDSLCELHIKQGRYELAAFL
                     ACNLKE"
     gene            complement(545..757)
                     /gene="cspA"
                     /locus_tag="OO433_003310"
     CDS             complement(545..757)
                     /gene="cspA"
                     /locus_tag="OO433_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6051668.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1038..1328)
                     /locus_tag="OO433_003311"
     CDS             complement(1038..1328)
                     /locus_tag="OO433_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR6051669.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGISVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1761..2471
                     /locus_tag="OO433_003312"
     CDS             1761..2471
                     /locus_tag="OO433_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6051670.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPVAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2521..3495)
                     /gene="ghrB"
                     /locus_tag="OO433_003313"
     CDS             complement(2521..3495)
                     /gene="ghrB"
                     /locus_tag="OO433_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6051671.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKPSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3599..4258)
                     /locus_tag="OO433_003314"
     CDS             complement(3599..4258)
                     /locus_tag="OO433_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6051672.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLDSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4411..6744
                     /gene="bisC"
                     /locus_tag="OO433_003315"
     CDS             4411..6744
                     /gene="bisC"
                     /locus_tag="OO433_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6051673.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVHVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVHAAKIRELAALFHQNTTMLMAGWGMQRQQLGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRCSAVLSSMQGSLPGGSDAVDK
                     IPVARIVEALENPGCAYQHNGMNRLFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDALARGIQD
                     GDTVRLWNARGQILAGVVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6713..7153)
                     /locus_tag="OO433_003316"
     CDS             complement(6713..7153)
                     /locus_tag="OO433_003316"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6051674.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7150..7713)
                     /gene="tag"
                     /locus_tag="OO433_003317"
     CDS             complement(7150..7713)
                     /gene="tag"
                     /locus_tag="OO433_003317"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6051675.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7871..8569
                     /locus_tag="OO433_003318"
     CDS             7871..8569
                     /locus_tag="OO433_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6051676.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8797..9999
                     /locus_tag="OO433_003319"
     CDS             8797..9999
                     /locus_tag="OO433_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6051677.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2427 a         2435 c         2591 g         2664 t
ORIGIN      
        1 ttttttagga ttatatcaag gagaagaaac aaacttatta agctagaata gctacgggtg
       61 cttgagactg tttgtctcag gtattcaccg aaaggcagac agagaaaagc cccacctgac
      121 tataaatcaa agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt
      181 ggaaatcaac acagggggct gggaatgccg caaaaatacg gattactttc attaatagtg
      241 atttgtttca cgcttttatt tttcacctgg atggtaagag attcactgtg tgaattgcat
      301 attaaacagg ggcgttatga gctggcggca tttttagcct gcaatttaaa agagtaagag
      361 tcttcggcgg gaaattattc ccgcctttct tacggcgttg cgcattctca ttgcacccac
      421 atttattctt cacaaaaata ataatagatt ttactacgcg atcgattatt tatttcctga
      481 aaaaaaataa aaaaaatccc cgccaaatgg cagggatctt agattctgtg cttttaagca
      541 gagattacag gctggttacg ttaccagctg ccgggccttt agcgccgctt tcgatggtga
      601 aggacacttt ctgaccttcg tccagagatt tgtaaccatc gttctggata gcagagaagt
      661 gtacgaacac atctttagag ccatcgtcag gagtgatgaa gccgaagcct ttgtcagcgt
      721 tgaaccattt tacgatacca gtcattttac cggacatagt gtattacctt taataattaa
      781 gtgtgccttt cggcgatatg gcgtgcttta cagattttga agcgttaaag gaatgtgcac
      841 tacgaggggt atcaacgata actcttgaag ggacttgcct tactacactg ctttaatggt
      901 ctgtacgtca aaccgttgat gtgcattaag ccacgcattg gcgggtgatg caacaattat
      961 ttttcatatt tatgattaat cggttggaaa acggtcctgt catcaggacc gtaaacagca
     1021 ataaagtgga taaaagtcta ttccatcaac tgcttactta atgccgggtt ggcttgaatc
     1081 aaacgcatca attttagttc ggcacttgaa ggcttcacgc gtctggattc ccattccttt
     1141 accatggcga ctgatatgcc caaaacccgg gcgaaatcat cgatttttaa ccctgtccct
     1201 tttcgtaact gctcaatttc ggtacaggac gttgttctgt gcgtcagggt aattttctgc
     1261 gtttcatctt taaaaacaat ctgttccagg ctgctcaaca gctcatgcat tggatcttta
     1321 tattccattg aaaactcctc aaatcacact gcgggatcgt gaattgcatc gaagctcatt
     1381 aagaatagtc gggaaaagca gacacagcgg ggtgaagtca gggcatgctc tggctatgat
     1441 tcgattcagc cagtcattta gcgtgactta tagccaccta acgatctgat aaatgccgat
     1501 atatctctta ccactcctgt tttgtaaaga tttcgaaaat ctaaagttct gcgaaggcgt
     1561 aaatctcggg ctacaaaaaa gcaacggcaa tatcattttt taacaatgaa acaacaaatt
     1621 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1681 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1741 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1801 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1861 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1921 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1981 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     2041 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2101 tgaacggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2161 ctgcgcactc tgcgctaaaa cagaccgatg atttgaaacc ggtctttgat caggcattca
     2221 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggtt gcacaaactt
     2281 tcacgcaaca actggtgatg gttggagact acattgctca acaaggtact caggtgagct
     2341 ttgtagcaaa tggtatccag ttcccgactt cgcagcaggc aagtgaatat aacaaactga
     2401 ttgcgccgtt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2461 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2521 ttagtccgcg acgtgcggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2581 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2641 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2701 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2761 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg agggtttcat
     2821 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2881 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2941 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     3001 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3061 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3121 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3181 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3241 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3301 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3361 ttcggcaaaa attgctgcat tttgttcgac ggtttgtggg ctgaggtttg ccacctggtg
     3421 aacggtgaaa tgctcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3481 aacggacggc ttcatgcttc tctccattca ctgataaggc gctcacctta gcctgaaata
     3541 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3601 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3661 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3721 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3781 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3841 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc agggtcgcgc
     3901 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3961 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     4021 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4081 cgcgatcttt cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagtccagac
     4141 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4201 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4261 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4321 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4381 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4441 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4501 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4561 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4621 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc acgtgagttg ggatgaggcg
     4681 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4741 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4801 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4861 gcggcacagg cgatcatgcc gtatgtcgtg ggtggcagcg aagtttatca acagcaaacc
     4921 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4981 aatacgctga aaattgcgtg gaatgcatct gatgagcagg ggctttctta cttttccgca
     5041 ctgcgtgaca gcgggaaaaa gctgatctgt attgatccaa tgcgatcgga aaccgtcgat
     5101 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5161 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcgt
     5221 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcc
     5281 aaaaccgccg aatgggcggc agggatttgt ggggttcacg cagcgaaaat tcgcgagttg
     5341 gcggctcttt tccaccaaaa cacgaccatg ctgatggctg gctggggaat gcagcgccaa
     5401 cagcttggtg agcaaaaaca ctggatgatc gtcacgctgg cggcaatgtt ggggcaaatc
     5461 ggcacacccg gcggcggttt tggtctttct tatcattttg ccaatggtgg taatcccacg
     5521 cggtgttctg cggtgctctc ttccatgcag ggtagcttgc cgggtggcag cgatgcggta
     5581 gataaaatcc ctgttgcccg tattgttgaa gcactggaaa accctggctg cgcataccaa
     5641 cacaatggca tgaatcgcct cttcccggat attcgcttta tctggtgggc gggtggcgcc
     5701 aacttcaccc atcatcagga cactaatcgc ctgatacgag cttggcaaaa accggagctg
     5761 gtagttatct ccgaatgctt ctggacggcg gcggcaaaac acgcggatat cgttctgcct
     5821 gcgactacct cttttgagcg taatgatctc accatgaccg gcgattacag taatcagcat
     5881 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5941 tttgccgagt taagtgaacg ctgggagaag ggcggttatg cacgttttac ggaaggaaaa
     6001 agtgagttgc aatggctgga aacgttttat aacgttgccc gacagcgcgg ggcaagccag
     6061 caggttgaat tgccgccatt tgctgagttc tggcaagcca atcagttaat tgagatgccg
     6121 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgcaggcg
     6181 catccattaa aaaccgccag cggtaagatt gaaatcttct cgcagcgtat tgccgattac
     6241 gcttatccgg attgcccggg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6301 gccgaaccgg aacagttaca ggtactttct gctcatccgg cgcatcgtct gcacagccag
     6361 ctgaattaca gttctctgcg cgaattgtat gcggtggcaa atcgtgagcc tgtcaccatt
     6421 catcctgacg atgccctggc gcgcggcatt caagatggcg atactgttcg gctgtggaac
     6481 gcacgcgggc aaattcttgc cggagtggtc attagcgagg gaattaaacc tggcgtgatt
     6541 tgtattcacg aaggggcatg gccggatttg gatttaaccg ctgacggtat ttgtaaaaac
     6601 ggcgcagtca acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6661 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6721 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6781 aatcgacaat gtgaaaaccc tgcgcctggt aaaaatttat cgccggttga tttttttgat
     6841 aaacctccag catcaggtgg ggatggcgct gctgcacata ttgcatcagc gccttaccaa
     6901 taccgcgcct gacggccttc ggtgcgacaa acatcgctgc cagaaatcgg ccttccataa
     6961 tgctgacaaa accgagaagc ttactgtctt cttcccagac ccagttttgc gcattggcaa
     7021 gatacgcatc ccgcaccagc ggaatgcaat cacgccagta attcgctttt ataaagggat
     7081 gtccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7141 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7201 cagcccacat gcctgcataa aggagtaaca gattgtggta ccgacaaact taaaaccacg
     7261 ttttttcagt gctttagata gggcgtcgga ggcgggcgta gatgtgggga tttcgctcaa
     7321 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcggcaaa
     7381 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7441 tttcccgcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7501 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7561 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7621 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7681 cgggtcctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7741 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7801 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7861 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7921 cgtggtaatc agcgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7981 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     8041 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8101 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8161 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac
     8221 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8281 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8341 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8401 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8461 tatgttgctc aatcaaaata ttgccgccta tgccgcgcta actcaggcag aaaataccac
     8521 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8581 caattgaaac cattgaataa caatagttgt ggtgatagtg ggtgctaact tacaaataat
     8641 aaatttggtg aataattgtc gcgtcattca ttcctgaact aaggcatttc attccgttct
     8701 gatggcattt catgccgttt ttccccaggc ataaaatgca cttcgttatg gttgtcggca
     8761 gagatttttc ctttttatta ctgcaggaat actgccatga caccttcaaa ttatcagcgt
     8821 acccgctggc tgacactcat cggtactatc attacccagt ttgcgctggg gtcggtttat
     8881 acctggagcc tgtttaatgg cgcgctttct gccaagctgg atgcgccggt aagccaggtc
     8941 gctttctctt tcggcttgtt aagtctgggg ctggcaattt catcttctgt tgcggggaaa
     9001 ttacaggaac gttttggcgt taaacgcgtc actatggctt ccggcatttt gctaggatta
     9061 ggcttcttcc tgacagcgca ttctgacaac ctgatgatgc tgtggttaag cgccggtgtg
     9121 ctggtcggtc tggcggatgg cgcgggttac ctgctgacgc tctctaactg cgtgaagtgg
     9181 ttcccggagc gtaaaggtct gatctccgcg ttcgctatcg gttcttatgg tcttggtagc
     9241 ctgggtttca aatttatcga cacgcagctg ctggaaacgg tgggtctgga aaaaacattt
     9301 gtgatttggg gagcgattgc gctggtgatg attgttttcg gcgcaacgtt aatgaaagac
     9361 gcaccaaaac aggaagtgaa aaccagcaat ggtgtggtgg agaaagatta cacgctggca
     9421 gagtcgatgc gtaaaccgca gtactggatg ttagcggtaa tgttcctgac tgcctgcatg
     9481 agcggcctgt acgtgattgg ggtagcgaaa gatatcgccc aaagtctggc acaccttgat
     9541 gtggtttccg cagccaatgc agtaacggtt atttccatcg ctaacctttc aggtcgtctg
     9601 gtgctgggca ttctgtctga caaaattgcc cgtatccgtg ttatcaccat tggtcaggtg
     9661 atttcgctgg tgggtatggc ggccctgctg tttgcaccat taaatgcagt gacgttcttt
     9721 gcagcgattg cctgcgtggc atttaacttt ggcggcacta ttaccgtctt tccatcactg
     9781 gtcagtgagt tcttcggcct caataacctg gcgaaaaact acggtgtgat ttacctcggt
     9841 ttcggtatcg gtagcatttt tggttcgatt atcgcctcac tgtttggcgg tttctatgtg
     9901 accttctacg tgattttcgc cctgctgatt ctgtcattgg cgctttctac gacgattcgt
     9961 cagccagagc agaaaatgtt gcgtgaggcg catatttaga aatatgttgc tactgaaata
    10021 aatacgagga gggagacttc aaagtctccc ttttttatgg acgcatatta aaagaacaat
    10081 aatattcata ccacaaatat attttattta tatatta
//
LOCUS       DAJTSL010000149         9994 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTSL010000149 DAJTSL010000000
VERSION     DAJTSL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.149"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(272..1636)
                     /gene="tdcG"
                     /locus_tag="OO433_003320"
     CDS             complement(272..1636)
                     /gene="tdcG"
                     /locus_tag="OO433_003320"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6051678.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGALSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1708..2097)
                     /locus_tag="OO433_003321"
     CDS             complement(1708..2097)
                     /locus_tag="OO433_003321"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6051679.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(2111..4405)
                     /gene="tdcE"
                     /locus_tag="OO433_003322"
     CDS             complement(2111..4405)
                     /gene="tdcE"
                     /locus_tag="OO433_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6051680.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFEAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNIMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4439..5647)
                     /gene="tdcD"
                     /locus_tag="OO433_003323"
     CDS             complement(4439..5647)
                     /gene="tdcD"
                     /locus_tag="OO433_003323"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6051681.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5673..7004)
                     /gene="tdcC"
                     /locus_tag="OO433_003324"
     CDS             complement(5673..7004)
                     /gene="tdcC"
                     /locus_tag="OO433_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6051682.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(7026..8015)
                     /gene="tdcB"
                     /locus_tag="OO433_003325"
     CDS             complement(7026..8015)
                     /gene="tdcB"
                     /locus_tag="OO433_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6051683.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(8114..9052)
                     /gene="tdcA"
                     /locus_tag="OO433_003326"
     CDS             complement(8114..9052)
                     /gene="tdcA"
                     /locus_tag="OO433_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6051684.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRLLIEVG"
     gene            9241..9585
                     /gene="tdcR"
                     /locus_tag="OO433_003327"
     CDS             9241..9585
                     /gene="tdcR"
                     /locus_tag="OO433_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6051685.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         2690 a         2516 c         2272 g         2516 t
ORIGIN      
        1 tttctcgctc aatattttgt agggcttatt atttttttcc gagccgcatc aaggcgatat
       61 gcggtctacg tttttgcagg cgagttatta gaataaagaa atgatacgcg cgaggtcaga
      121 gaaaatgttc acaatcaaga ctttgaaata acggagatgg ataagaattt tctactaaat
      181 taatcgcaag agaatattca ttctctattt gcggcagatc acaaaaaaag gtgcacattt
      241 gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc
      301 ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
      361 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg
      421 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
      481 cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc
      541 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg
      601 ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat
      661 acagcgcgcc aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg
      721 gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag
      781 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
      841 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct
      901 gacgacgcag cgctacggca cgacgcggca cattgagcgg accaggcagc acgccttcag
      961 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaccctgcgt
     1021 caatttccgc tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca
     1081 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
     1141 acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
     1201 cagagtaata ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc
     1261 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
     1321 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
     1381 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa
     1441 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgccccatac agatcgacca
     1501 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
     1561 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
     1621 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
     1681 ccttgcgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc
     1741 cagcttcaca tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc
     1801 ctgatgctca tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc
     1861 ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
     1921 cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc
     1981 ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc
     2041 ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
     2101 tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac
     2161 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
     2221 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattatgttg acgttgaggt
     2281 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
     2341 tagttttacg tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg
     2401 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
     2461 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
     2521 gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca
     2581 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
     2641 agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
     2701 gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca
     2761 cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc
     2821 atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
     2881 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
     2941 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg
     3001 gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
     3061 agagcagcgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
     3121 ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca
     3181 gatcgtcatt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt
     3241 tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc
     3301 ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt
     3361 ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat
     3421 caaattccgg tgtacgcagg aagcgtacca tacggatctt catgatgaag tgatcgatca
     3481 gttcctgtgc ctgctgctca ttgagtacgc cagcttcaaa gtcgcgctca atgtagatat
     3541 cgaggaacga tgccgtgcgg cccagcgaca tcgcgccgcc attttgcgat ttcactgccg
     3601 ccagataagc gaagtagagc cactgcaccg cttcttgcgc attctgcgcc gggcgagaga
     3661 tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg
     3721 ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact
     3781 ggagatcggc aaattgcagt tcgcgttcac gtaccagata actgatgcca tacagcgcta
     3841 cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca
     3901 cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
     3961 gggttttacg cagatcggta aacagatatt caaattcact gtccatttca cggccatagg
     4021 cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtagcgca cgtttcaacg
     4081 gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat
     4141 gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt
     4201 cgatacggat accttccatt actttttccc acaattccgt ggtggcaggc gtcgcttcgg
     4261 cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
     4321 taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat
     4381 cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt
     4441 atgcaaattc tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt
     4501 tttcttcgtt agtcggaata acagcgcaaa tgacacgcgc attttcactg gaaacaattc
     4561 gctcaccaca ggagttagag cgattattca tttctgtatc aatctctacg cctaatacag
     4621 ccaaatgttc catgaccaga cgacgaatta agcttgaatt ctctcctatt ccgccggtga
     4681 atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc
     4741 ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt
     4801 ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca
     4861 ctacgcgttc caagtcaccc aagctctggt tggtttggct ggcgacccag gacatcgcac
     4921 caaaatcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca
     4981 ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca ttgccaagat
     5041 gcgccacaac caggccggag tcatcttccg ctagattcag caaagaatgt gcgcgctggg
     5101 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
     5161 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
     5221 caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
     5281 aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgacct
     5341 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
     5401 ttaaggccac actgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
     5461 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
     5521 cattttctga gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat
     5581 cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
     5641 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg
     5701 atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
     5761 agagacggtg ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc
     5821 ggtgcgccca tggcttcaat aaggtcgagg atgttcgggt tggcgtaggc aacaacccag
     5881 gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga cactttagtt
     5941 ttgtcgcctt tataaccgaa cttcagaatc agaccattca agccttccag cgtccccaga
     6001 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
     6061 agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa
     6121 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaac
     6181 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
     6241 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt
     6301 ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa
     6361 atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
     6421 tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc
     6481 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
     6541 aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc
     6601 ggtgcaaatc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
     6661 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
     6721 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
     6781 agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac
     6841 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
     6901 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
     6961 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
     7021 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
     7081 ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat
     7141 aatgcagcac atgccagagc acctgcgcct tcggtgacga ctttattgcg ctgaattaag
     7201 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
     7261 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
     7321 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
     7381 ttttctgact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
     7441 ataccagcaa ttaaaccacc accaccaatt ggcacaatca cgttatcgac atcatagaga
     7501 tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa
     7561 ggtgggataa aaatacgacc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
     7621 ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
     7681 gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
     7741 agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc
     7801 gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
     7861 tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag
     7921 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatatca
     7981 tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
     8041 atatgtagat aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa
     8101 taaaaacaaa taactaacca acttctatta atagccttcg tctacaccca ttataagatg
     8161 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
     8221 accataccgc ggcatattgt gccacaggta atgtttcttc aactggaata gtaataaatt
     8281 gatttgaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga
     8341 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
     8401 catttctttg taacgtagta agaagctcgc tgtagtaccc catattggtt tgtggcaaca
     8461 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
     8521 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
     8581 tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga
     8641 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
     8701 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
     8761 ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg
     8821 taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat
     8881 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
     8941 cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga
     9001 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
     9061 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc
     9121 gaccactcac aaattaactt tcattacttt ccagggtatc gttattacaa ttaaatatct
     9181 ttaaatatca acaagttaaa acataaaaat cagcataaac cctgattttt atcaaaaaaa
     9241 atgacgggga taaccatatt ttatggcgat aacatcattc gttatgtggt taatacaaaa
     9301 aaggggctga gaccatattt caagcaatta ccggataatt accaggcaaa atttgagtta
     9361 aatttaatga gcaagttctc aaattttatt ataaataaac cattttcagc gataaatact
     9421 gcggcacgtc acattttttc acgctatttg ttggagaaca aacatttatt ttatcaatat
     9481 tttaaaattt cgaatacagg tattgatcat ctcgaacaat tgattaacgt caactttttc
     9541 tcttctgaca ggacgtcatt ttgtgaatgc aatcgttttc cataaattct tctcccctca
     9601 taggcgacga atagcatttt gtgttgagga tcacaaaaca aataattgct ggtcgccgcg
     9661 ataaggtcag acgaagtcaa caaggggaat tttcactgtg ctttacatca gcgtaggaca
     9721 cagaatgttg cttcgtttac tgtaacgccg ggtaaatgag cgttttttga tagtgcgtaa
     9781 gtgtcgtgcg aggaaaataa gcatctatta ttgtttgtca tgacgacaag gcaggcatca
     9841 atgaaaggct tcccaattgc acatattttt cacccttcaa tcccgccaat gcacgcagtg
     9901 gttaacaatc acaatagaaa tattgattat tggacggtaa aaagaaagtt tgcagaaatt
     9961 gtctccacca atgacgttaa aaaaatttac agta
//
LOCUS       DAJTSL010000150         9991 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTSL010000150 DAJTSL010000000
VERSION     DAJTSL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.150"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(266..1609)
                     /gene="gdhA"
                     /locus_tag="OO433_003328"
     CDS             complement(266..1609)
                     /gene="gdhA"
                     /locus_tag="OO433_003328"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6051686.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSESEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVGAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1845..2117
                     /locus_tag="OO433_003329"
     CDS             1845..2117
                     /locus_tag="OO433_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6051687.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2083..2490)
                     /gene="nudG"
                     /locus_tag="OO433_003330"
     CDS             complement(2083..2490)
                     /gene="nudG"
                     /locus_tag="OO433_003330"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6051688.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPTDIPLLEAFMALRAARAAD"
     gene            2577..3188
                     /locus_tag="OO433_003331"
     CDS             2577..3188
                     /locus_tag="OO433_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6051689.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFISTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(3197..4504)
                     /gene="ynjE"
                     /locus_tag="OO433_003332"
     CDS             complement(3197..4504)
                     /gene="ynjE"
                     /locus_tag="OO433_003332"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR6051690.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(4571..5224)
                     /locus_tag="OO433_003333"
     CDS             complement(4571..5224)
                     /locus_tag="OO433_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6051691.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5224..6759)
                     /locus_tag="OO433_003334"
     CDS             complement(5224..6759)
                     /locus_tag="OO433_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6051692.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCTLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALAAKWVGYVRQ
                     GLR"
     gene            complement(6732..7898)
                     /locus_tag="OO433_003335"
     CDS             complement(6732..7898)
                     /locus_tag="OO433_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6051693.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WDDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(7908..8456)
                     /locus_tag="OO433_003336"
     CDS             complement(7908..8456)
                     /locus_tag="OO433_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6051694.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8456..9163)
                     /locus_tag="OO433_003337"
     CDS             complement(8456..9163)
                     /locus_tag="OO433_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6051695.1"
                     /translation="MMKMQSRKIWYYCITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(9178..9855)
                     /gene="ydjY"
                     /locus_tag="OO433_003338"
                     /pseudo
     CDS             complement(9178..9855)
                     /gene="ydjY"
                     /locus_tag="OO433_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
     gene            complement(9860..>9991)
                     /locus_tag="OO433_003339"
     CDS             complement(9860..>9991)
                     /locus_tag="OO433_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6051696.1"
                     /translation="LQLCLAGLALFILVQLAKLYARHKHVDLSASRRSPLTHPKNEG"
BASE COUNT         2378 a         2724 c         2584 g         2305 t
ORIGIN      
        1 atatctgctg ctatcgcaga aagttaacgg atgctgaact tgaagcgtgt tatcgttacg
       61 acaagcagtt ttgggagcac tacagtaaga aatgccgttg gcccataaga aatgttccct
      121 ggggagcatc atccgttaaa tactcataaa cgcctgaaat tttgccgggg gcgctgcgct
      181 tgcccggcct acattagagc gtaactgcaa tgaattgtgc ccatttgtag gcctgataag
      241 cgtagcgcca tcaggcattt acaacttaaa tcacaccctg cgccagcatc gcatcggcaa
      301 ccttcacaaa accggcaatg ttcgcgccct gcacgtagtt ggtttgctca ccttcaccac
      361 catgctcaac acaggcatgg tggatatcca gcatgatgtg atgcaaacgt gcgtcaactt
      421 tctcggcttt ccagcccagg cgcgcagcgt tttgcgccat ttcaaggcct gacgttgcta
      481 caccgccagc attagccgct ttacccggtg caaacagtac gcctgcctgc tggaacagtt
      541 cagtcgcttc gatggtggtc ggcatatttg ccccttcggc gacggcttta acgccattgg
      601 cgataagctg atgcgcggcg ccaacatcca gttcgttctg ggtggcgcaa ggcagggcga
      661 tatcaaccgg tacagaccac ggctgttggc cttcgagata gaccagacca aattctttgg
      721 cgtaatctgc cactcgacca tcgcggctgg ctttgatttc gataagacgt gccagtttct
      781 ctttcgtgaa tccgctttca tcaactacag tgccgctgga gtctgacgca gtgatcacac
      841 gagcaccaaa ttccatcgct ttttcgatag cgtactgggc gacgttgccg gagccagaaa
      901 cggaaacgcg catcccttca aaacccatac cgtggcgttt taacattgct tctgtgaaat
      961 aaaccagacc gtagccggta gcttccgggc gaataagact accgccaaat gaaaggccct
     1021 taccggtgaa gacgcaggcg gtattgttgg agagcttttt catcatcccc gccataaagc
     1081 cgacttcacg accaccaacg ccgatatcac ctgccggaac gtcagtatcc gcgcccagat
     1141 gacgataaag ctcggtcatc agcgcctggc agaaacgcat cacttcgctt tcgcttttac
     1201 ctttcgggtc aaaatcgcta ccgcctttac caccgcccat cggcagggta gtcagggcat
     1261 ttttgaaggt ttgttcgaag ccgaggaatt tgagaatgga gaggttaact gacggatgga
     1321 agcgcatacc gcctttgtac gggccgatgg ctgagctgaa ctgcacacgc catgcacggt
     1381 tgacctgtac ctggttgcga tcatcaaccc ataccacgcg aaactggatc acgcgctccg
     1441 gttcaaccag acgctccagt aatgacatct ggcgatattt tggattttgt tcaagaaaag
     1501 gccagagtgt ggtcattact tcacgaacgg cttgcgcgaa ctcggtttga ttcgggtcgc
     1561 gcttttggac atggttgagg aatgactcca gagaatatgt ctgatccata gatataaaac
     1621 ccttatatat taatacgatt gtgcttatgt tgtttatgtc atgtgctttt gcagttttcg
     1681 actataccat caagaatgtg acggtaagca agaaaaaaat gacccaggaa agcaaattaa
     1741 taacgagagt aatctcataa ccaaatgcga taactcagac gctaaagttt tgccacccgg
     1801 ctaccgttat cagttgcaga gacaataacg ttaaggagtt ccctgtgagt cgagcattgt
     1861 tcgccgtagt attagcattt ccattaattg cactggctaa cccgcattat cgtccggacg
     1921 tggaggtaaa cgtaccgcca gaagtgttca gttcaggcgg gcagtcagcg cagccctgta
     1981 ctcagtgctg cgtttatcag gatcaaaatt attctgaagg ggcggtaatt aaagccgaag
     2041 gaatactgct gcaatgccag cgggatgaca aaacccttag cactaatccg ctgctctggc
     2101 ggcgcgtaaa gccataaacg cctctaatag tggaatgtca gtaggggcca gcggatattg
     2161 cagcgcctct tcaggtgagc accagaccag cgcctgatgt tcatgtgcct gtaacgtccc
     2221 gtggaagtcg ggtacgtgcc aggcatgaag atggataatc cggcccgaaa cttctcgctg
     2281 atggctggca acatactcac ccacagttgc ttcgatgccc aattcttcgt ttaactcacg
     2341 caccagtgcc tgctgctggc tttcatccag ctcgacttta ccaccggcaa actcccataa
     2401 tcccgcctga tcgctctggg cggggcgttg cgcgagtaaa attttgccat cacgttcaat
     2461 gatggcggca acaacttcaa tcattttcat aaattacaca cgggcagata agcgaaaaat
     2521 gccataatat ccgcactgtt gctcaatcag aaaggcttta tatttaagga gtgcctgtgc
     2581 tagaccgcca tcttcatccc cggattaaac cgttgttgca tcagtgcgtg cgggttcttg
     2641 ataaaccggg cattacgcca gatggtttaa cattagtggg atttgccatc ggcgtgctgg
     2701 ctctgccgtt tctggcgctg ggctggtatc tggcggcgct agtcgtcatt ttgttgaaca
     2761 ggctgcttga tggtctggac ggtgcgctgg cgcggcgcag agagcttacc gacgcgggcg
     2821 gctttcttga tatttctctc gattttctct tttacgcgct ggtgccgttt ggctttattc
     2881 ttgctgcacc ggagcaaaat gcgctggcag gcggctggtt gttgtttgcg tttatcagca
     2941 cgggcagcag ttttctcgcc tttgctgcac tggcagcaaa acatcagatt gataaccccg
     3001 gttatgcaca taagtcgttt tactatctgg ggggattaac cgaaggcaca gaaacgatct
     3061 tactgtttgt gctgggatgt ttattccctg catggtttgc gtggatcttc ggtgcgttgt
     3121 gctggatgac gacctttacg cgggtgtgga gtgggtatct gacgctgaag tcgctccagc
     3181 gtcagtaatt gcaatgttat ttgctactgt ccgggccgcg ttcaccggtt gctaccggat
     3241 tttttggatc gctgctccat tcgtaccagc cgccgtcata cacggaaaca ttcttccaac
     3301 ccatggcgcg tgcgtacata aaggtttcgg acgcgcgcca gccggtgccg cagtagaatg
     3361 aaacttgctg ctctggtttg atattccacg ctttccacat agcggtaata tcatcggcgc
     3421 tgcgcatggt gccatccggg ttatgaaaat cttccatatg cgtcgagtcg ctaccagcgt
     3481 gtccccaacg tgctccggct atttcacctt ttggtttaat atagctgtaa ccgctggtcg
     3541 taccgataaa ttctggccac gaacgaatgc tcaccagcga tgcatcctgg cgatgcagca
     3601 gtccacgggc ctgttccata tcgagcatca actgcggttg tgccgggatc ttcacgccga
     3661 aatccggttc cgctttcact ttcggtggcg ttcctcgctc aacaggcagt cccgcgtcgg
     3721 accaggtttg ccagccgcca tccagcagac gcacatcttt cacgcctgcg tagagcataa
     3781 tttgcgccac acgcgctcca gcgtatacgt cacgcccgta cagaatgacc gtggtgtcat
     3841 ggcgaatgcc gtgttttgcc agcatcgctt ttagttgttc atcagaaact ttgttccaca
     3901 gcggttcgct ttccacttcg ttggtatcga tgtagtcagc accgggaata tggctgataa
     3961 ggtaaagctt aggagcgccc caggccgctt caatgacttt ccagtcaccg gcaggtttcg
     4021 ccgtaacctc tttaccttgt tgcaggtcgt gcagccattg cggataaacc agctgctcaa
     4081 agtgcggcag tttttgtaga cgggaaggtt cgcttagcgc gtcactcaga atggagatat
     4141 gcgtaaaacc tgctttttgc agtcgcgttt tgacggcgtc gacatctttg tcattaccgt
     4201 acagcgccac cggggcatcg gctttcaggt tatgttgctt gatccacgcg ttgagctgtt
     4261 cggtgctcat tttgtcgagc cagctggcag agaggtttaa ggcggcaggt tcatgaccag
     4321 aagggccatt taaggtttgt ggccagccgt tataaaacgc gctggggcga gtatctatcg
     4381 ctttgccatt ttgttgttga agctggtcaa gtgtaagagg cttcgccagt tcagcggccc
     4441 acgaagaagc gcaagccagc cctaaagcca ttgccagcgc ggtcatttga gaaacacgtt
     4501 tcatcgaaaa tcccgacatt gtagttttga gtgcgccatt ctgaactgac gcattgagaa
     4561 aaactttgcg ttatcgcagt ttgttgtaat tttctgacca ctgcgccata tccagaacag
     4621 aactatcagc aggaacatcc tggagatcgt gcgttacctg aacgacgggg atcgccagtt
     4681 cgcgaacttc gctgaacacc cactggcgaa aattatcgcg cagagccaca tcaagacggc
     4741 tgaatggctc atccaggagt aacgcttttg gctgagcgag aagggcgcgc agcagggcca
     4801 cccgcgctcg ctgaccgcct gataatgtcg cagggtcctg gtgataagtt tcagccagtc
     4861 ccgctcgatc aagcgcgtct ttcacggcat tgcgcctggc agtccccttg agcgtcgacg
     4921 gtagcgccag cagtaaattt tgcccgacgc tgaactggtc aaataacagt gcatcctgga
     4981 aaagaatgcc aatctgacgc tgtgcggtgg gtagcatgtc aatccgttgc tcattgagcc
     5041 atagctcacc tgtacaagaa aactgtccag ccagtgcacc aatcatccat gaaaacagag
     5101 tggattttcc acagccagac ggccccatta acgtgacgat gtcaccttta tcaaccgtaa
     5161 agttaacgtt tgtcagcaag cggctttctg gtaaacgtag cgaaacattt ttcacgcaga
     5221 gcattagcgg agtccttgtc tgacataacc tacccatttt gcggctaacg cggtcagggc
     5281 aaaaataata agcggtaaca acagttgcca taaagcctgg gcggcgagaa taccgttgct
     5341 gccgccgctg cttaatgcca ccgcctcagt ggtgagcgtc ggaaaacgcc ccgcgcccag
     5401 ccacagcgtc ggcatatact gcgcaatact gactgaaaat cccaccgcga aggcaatcag
     5461 cacagggcgc aacatgagtg tgcatttcac gtaaaagaag attttggccc gcgaccagcc
     5521 cagtgtttgc gcaatcaaaa ttaaccgtga atcaatgcgc tgccaggcgg gttgcaggat
     5581 aaacagcatc cacggcatta cccacagcag atgcccccag accaccgccg tccagcttcc
     5641 atccaggttc agccatagcg ccagcgtgta ctggcccgcc accagtggca gagcgggcaa
     5701 tagaatgggc aaccatagcc acaactggcg acgctgtggc ccccattcca gccacagcag
     5761 taacaggagc aaagcgatga atgttgctac cagccccatt gtcagactgt tgatgagtgc
     5821 ttcactattg atcgtcgact gatccgcgag gatcgccagc agaaccacac acagcacacc
     5881 ggttaagggt aaaaaactcg ccagcgtagt gcctggtaat aaaggtgtgg caggcttgcg
     5941 aatgccatct acgcggggaa tagtgcgccg ccagctgcgc catagcagat agctcagcaa
     6001 aacgtaggcg gcgagtaaca gcatcagcag cagactggcg agtgcgcctt tcgtttgttg
     6061 atccgcgtcg ccctgagtta accactgcca gctaattact gccagcgtcg gtggattacc
     6121 tggcccaaga ataatcgcca catccacgac cgaaagcgac caggcaacaa tcgccagcat
     6181 cgccattgcc agcgaaggcg cgacggaggg caacagcagc cagttcaggc attgccagcg
     6241 gctgtagccc agcgaatcca gcacaatgac ctgctgcaac agccgttttt cgctcaatac
     6301 tgccgctaag atccacagca gaaatgcgct ttctttcact gcaagggtga ggcccagacc
     6361 gatgccaaat cggtccattg gcggagtgaa atacgggaaa tagtcatata gcagccctcc
     6421 gtcagcaaag agcagcaggg cgctggtggc aaaagccaca tggggaatgg cgagcagcca
     6481 cggcagacgg gcgcacatac gctgccattt cggcccaggc catagcgcca caatcaccag
     6541 cagggcaatc aacaatgccc cgaccgccgc gatggttgtc gacaccagcg ttgccagtaa
     6601 tgcatgcggt aactgtggat cggcaaataa cgcctgccag tgtgtcaacg acaacgcagg
     6661 cgatatcagc gtgagagctg ccgggatcag cggtgcataa atcaccgcca ccatcgccca
     6721 cagcaaaaag attaatgcgt accgtaacgg cgtaaccatt cttgttccag tgcattcacc
     6781 catcctgcgt gcggttcagc cagtaccggc ggcagatcct gcggcattct tgattgcaat
     6841 atttcgcgct gcccgtcagg cagtttttgc ggatcgagaa cagaaggatc gtcccagaca
     6901 acgggatctg ctttacgcag ttgcgcatcg ggtgagagca gaaaattggc aactaccttc
     6961 gccgcagcac tggcattcgc gttggctgga atggtgacga aatgcacgtt gccaatcatc
     7021 ccctcgcgaa agccaaaact gtaactgctt gccggcaaat cgccgctggc gattttttgc
     7081 tgcgcatgcg cggggttaaa ggtcagcgac aggcgcaatg tgccggcttt cagcagagca
     7141 tccatccgcg cgggcgaagg cgggaaatcc tttccttcgc gccacaaata cggatgcagt
     7201 gcatcaagat attgccacaa gggcgcagtg acacgggcga aagtcgcatc gtccggcgct
     7261 tcttttaatg cggcgggatc tggcgtcagc ataatcagca actgttcaag aaacgccgtg
     7321 ccggtaaagt ccggtgggcg cggataagta accgtgccgg gattggcttt agcaaactcc
     7381 agtaaggctt gcggcgtttg tggtggctgt ggcgtaacat cgcggcgggc gataaacgtc
     7441 agttgtgcgc cgccccaggg cgattccgca ccttgtgtcg gcactgaaaa atcttcccgc
     7501 accggcagct gtgtgtcgac atagcgccag ttgggcagag tctccgccca gcccgtttgt
     7561 aataaattgg cctcttttaa ggtgcggaag ttttcgccgt tcacccagag cagatccacc
     7621 gagccgcccg ttttacgacc ggcagcagct tcggtctgaa tgcgcttcac cgcgtctgcg
     7681 gcatccgcga gacgaacaat cttcaggttt atagcgtaat gggttttcat ctcgccgcta
     7741 acccagtcga gatagcggtt aattgcggta tcgccgcccc aggcgttaaa ccagacggtt
     7801 tgccctttgg cctcattttt aatttcctgc cagtcactgg catgtgttgc caaagaaaac
     7861 agcgataaca atcccagcaa ccacccacaa tggcgcatct tctctcctta agaacgtttt
     7921 tccggaatac ggcacagccc ctgagcggga atgtccatgg cagaattaaa acgagccgac
     7981 aggttttgca ggccgatcaa tgccgtcagt tcggtgagcg cctgagcgtc aaaatgcgca
     8041 gccagtcggg tacgcagggc atcatcgact gttggcggtg tgacgcttgc ggcttcggcg
     8101 tactccagcg ccagccgttc ttcatcgcta aagagcgggc tttgccgcca gtcagccact
     8161 gccagcagtt tatcggtgct gccggtacgc tcggcgactt tcatactggt gatatccaca
     8221 caaaactcac acaggcacat ttgcgcaatg cgcgcgctga caagcgatcg cactaccgga
     8281 tcgagcggtg agcgtttgcg ctccagccac ccaacaaaca ttgacaccaa ataaaagata
     8341 aacgggatcc gcccccacca gcgaatcggg cttagcacct cgccgtaatg gcgacgatgc
     8401 agccacgcct gcggacgtaa aatgaaagga attttgctaa gcggcggtaa acccatcagt
     8461 cattcctctt ctggcgctca agccatattt tcttcgccat aaaaatcacc acggtcaggg
     8521 caaacagaat aaacagtccg gtgacaaacc agaaagtacc gcctgttaac atgctgcccg
     8581 cccaggaata aacaatagtc gccggtaatt gaccaagccc ggtggcgata aaaaacgagc
     8641 gaaaacgtat tgaagtcaga ccggcagcat agctgattgg atcgaaaggg acaaaaggca
     8701 ataaccgaca gaccaggatg gtgtgtttgc cgtagcgagt gaaaaagccg tccatactgt
     8761 caagcacggt tttgccggtt aatttttcca ccacttcgcg gcccatcact ctggcgataa
     8821 aaaagcacag cgccgcgccg gccatcgaac tggtccacga cagcaagccg ccccagaacg
     8881 cgccaaacag cgacgcattg gcaaaggtga tcaaaaacgc aggtagcggt gcagcaatgg
     8941 cctgtaaaat catcaacaag aacgagacaa ctgccgccag tgcgccgtaa gactgaataa
     9001 agcgttctat accctgttgg tccacggcgg caaacgccgc aacgctgcga ttgataaact
     9061 catggacgcc gggaagcagc gcccatgcga gcagcatagc gaacaacagg atgatgaggg
     9121 ttatacagta gtaccagatt ttacgcgatt gcatcttcat catcctttat cctggcttta
     9181 ttcggtgatt ttaaaggtaa ccgttgccag cgtgttatcc gccgggagaa ctgaggcatt
     9241 gcctttgaat ttcacttcgc cgcgtttttc caccgcacca taagtgtatg ttgcattgct
     9301 gacgatgccg accgggcagc tatccagaca caccagtcaa cccgttttct tctcttctgc
     9361 tgccgttaaa ttaccgccaa agcgcatgtc cagtttcttg ccattactgt caacaatcac
     9421 ttcatcgaag gaatacgctt ttgccgcccc ttgccagttg accgaaatat ccagtttgct
     9481 gcctgtgaca tgagtcgttt ctttattatc catcgtcatg ttttcgcccg gcgtaccacc
     9541 tgcctctttc agggcttcat aaaatgcttt cggggtcgca taagccataa acagcgattt
     9601 atgtccgttg gagccatctt taaacacaat accgtgacga gtgtcgtcgg tgaggaaacg
     9661 accattaatt tgcaccagca tggttaccgt atggttattg gcatcgacgc gcagcggttg
     9721 gctgttcgaa agtccgtcgt attccacttt tgccgccgaa ttctcttttt gatcgcagcc
     9781 gctaagcccg gcaacagcca gtaagcagag tgcagccagt ccttttttcc atgacactga
     9841 ataatgttgc gacatcgttc tatccttcat tttttgggtg agtaagcggg ctgcggcgcg
     9901 aagcagacag atccacatgt ttgtgtcggg cgtagagttt tgcgagctgg acgagaataa
     9961 acagcgccag gcccgccaga cagagttgta a
//
LOCUS       DAJTSL010000151         9854 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTSL010000151 DAJTSL010000000
VERSION     DAJTSL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.151"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..237
                     /locus_tag="OO433_003340"
     CDS             <1..237
                     /locus_tag="OO433_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6051697.1"
                     /translation="ETLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDML
                     VYEKTYTAELSSIAGTPIEAGPFDTVVLFKINYN"
     gene            389..1435
                     /locus_tag="OO433_003341"
     CDS             389..1435
                     /locus_tag="OO433_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6051698.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTCM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPDGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(1432..2223)
                     /gene="nei"
                     /locus_tag="OO433_003342"
     CDS             complement(1432..2223)
                     /gene="nei"
                     /locus_tag="OO433_003342"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6051699.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVGTRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2259..2993)
                     /gene="pxpA"
                     /locus_tag="OO433_003343"
     CDS             complement(2259..2993)
                     /gene="pxpA"
                     /locus_tag="OO433_003343"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6051700.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(2983..3915)
                     /gene="pxpC"
                     /locus_tag="OO433_003344"
     CDS             complement(2983..3915)
                     /gene="pxpC"
                     /locus_tag="OO433_003344"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR6051701.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(3909..4565)
                     /gene="pxpB"
                     /locus_tag="OO433_003345"
     CDS             complement(3909..4565)
                     /gene="pxpB"
                     /locus_tag="OO433_003345"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6051702.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIELLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4588..5331)
                     /gene="ybgI"
                     /locus_tag="OO433_003346"
     CDS             complement(4588..5331)
                     /gene="ybgI"
                     /locus_tag="OO433_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6051703.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5602..7083
                     /gene="dtpD"
                     /locus_tag="OO433_003347"
     CDS             5602..7083
                     /gene="dtpD"
                     /locus_tag="OO433_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6051704.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(7125..8543)
                     /gene="phrB"
                     /locus_tag="OO433_003348"
     CDS             complement(7125..8543)
                     /gene="phrB"
                     /locus_tag="OO433_003348"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6051705.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACSNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8540..9049)
                     /locus_tag="OO433_003349"
     CDS             complement(8540..9049)
                     /locus_tag="OO433_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6051706.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(9334..9708)
                     /locus_tag="OO433_003350"
     CDS             complement(9334..9708)
                     /locus_tag="OO433_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051707.1"
                     /translation="MFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIKYQKY
                     ENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAGRTCSLIFMADFGESRYFKPAKI
                     AMNKNNLIIRTSDVEIAFKITK"
BASE COUNT         2271 a         2690 c         2389 g         2504 t
ORIGIN      
        1 gaaacgttgg ttgaagaaga taaagcattg ttgattggta atggtctgaa gctacgtttg
       61 ctggatgaaa acgcctcgcc ttataccttc aataaatatg ctgaatacgc cgatttcacc
      121 agcgacatgt tggtctacga aaaaacctat acagctgaac tttcgtccat tgcaggtacc
      181 cccatcgagg ctgggccctt cgatacagtg gtgcttttta aaattaacta taactgattt
      241 gccatgatgc cctttgcagc cggaaagggc atctctttca gaaaattgct gattttcctc
      301 ttttccttga ttactgttat tttttcctgg cccggctttc tttatgctgt atcgatacac
      361 caaagagaaa ataatgaggg agcgaaggat gccagttttg cagtggggaa tgttatgtgt
      421 gctatcactt ctcctttcta ttggtttcct cgcggtacat ctcccggcgg cgctattact
      481 tgggccgatg atcgccggga tcatcttcag tatgcgcgga attactctgc aacttccccg
      541 ctccgctttt ctcgccgcgc aggccattct tggctgcatg attgcgcaaa acctcactgg
      601 ttcgattctc accacgctgg ccgccaactg gccgatcgtg cttgctattt tactggtgac
      661 gctgctttcc agcgccatcg tgggttggtt attggtgcgc tatagctcac tgcctggaaa
      721 taccggtgcc tggggttctt ctccaggcgg cgcagcggca atggtcgcta tggcgcaaga
      781 ttacggcgca gatattcgcc tggtggcgtt tatgcaatat ctgcgagtgc tgttcgtcgc
      841 gggggccgct gttctggtca cctgcatgat gctgggtgat aacgctgaag cggttaacca
      901 gcaaatcgtc tggttcccgc cagtgagcat taatctcctg cttacgattt tgctcgcagt
      961 cgttgccggt acggcgggat gcatgctgcg tcttccttcc ggcacgatgc tcatcccgat
     1021 gctggcgggc gcagtgctcc agtctggtca gctcatcacc atcgaactac cggaatggct
     1081 gctggcgatg gcgtatatgg caattggctg gcggattggt cttggtttcg ataaacaaat
     1141 cttactgcgg gcactacgcc cactaccgca aatcctgctg tcgatttttg ctctgctggc
     1201 tatttgtgca ggtatggcgt ggggactgac ccggtttatg catattgatt ttatgactgc
     1261 ctacctcgcc accagtcccg acgggcttga taccgtggcg gtcatcgccg cagggagcaa
     1321 tgccgatatg gcgcttatta tggcgatgca aaccctgcgc ctgttcagta ttttgctgac
     1381 ggggccagcc attgcgcggt ttatttcaac ctatgcgccg aagcggtcgg cctagtgctg
     1441 gcagcctgga caccagtaaa acgggcgcga ggacaacgtg gttttctcaa tgatgccgcc
     1501 acagcgttcg cacagttcgc catcacgatg aaaaacctta aagcgaaaca gcgccccatg
     1561 atgcttattc tcatccacct gcccccgcgt agcgtaagaa aaccgaggaa tctccagtaa
     1621 cgcgtgtgcg agtgcatcca gttgcgccgc attgagatct ttcgctttat gatttccagt
     1681 caacccaacc tgccagagga tctccacccg caaataattg ccaagcccag ccagaaacgc
     1741 ctgatcgagc agtaatcctg caaactgacg gttacgaaag cgcggtgaca ataaccgttc
     1801 ctttaccacc tccggcgtca gattcggatc cagcacatcg ggaccaacgc gttgtaaaaa
     1861 cggatgcgtg gtcagttgtt ccggcctcaa catctcaata tccgaggcgc tataaagcag
     1921 aatggttttg tcagccgttt gcagttttac ccgcaatact cgcgtggtct gcggctcttc
     1981 gccggtatca accacgcgcc agacgccgta aagctgatta tggctgtaga gcgttaagtc
     2041 gttggaaaaa tgagttaaca acgccttacc acgcgttccc acatgggtaa cgtgttgacc
     2101 gataagttgt gattgataag tttttaactg cgggaaggca aaccagacat cagttagtgg
     2161 tttgcctttg atcgccgcct ccaggttatc cgctgcacgg cggatctccg ggccttcagg
     2221 catggtgata tccttttttc attctttatt aatcggggtt atgctgcgac aacaatctcc
     2281 ttttcggcaa atgtagagcg tagtcggcgg gcgaacgcca gtgcgtgctc gccgtcgcca
     2341 tgcaggcaga ctgtttgcgc cgtgaccgtt gcccattcgc cggtgatact tttgactctg
     2401 ccgtgttgca ccatctctag cgtttgcgcc agcgcctgtt cttcgttttc aatcaacgcg
     2461 cctggctgac ttcgcggcac cagcgagccg tcagcctgat aaccgcgatc ggcaaacact
     2521 tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa tcagctcgct tcctgccagc
     2581 ccaacgagaa ccaatgctgg atcgcaagcg tataccgctc tggcgatggc gtctgccagt
     2641 tgtgcttctt tcgccgcctg gttgtacaac atgccgtgcg gtttgacatg acgcattacg
     2701 ccgccttgcg cacgggcaat cgttgccagc gcgccaattt gatacagcgt ctgggcgtaa
     2761 acggtttctg gcggcagctg catggcgctg cgaccaaaat tttccctgtc gggaaaactc
     2821 gggtgagcgc caatcgcgac accatttttt attgcttcac gcacgcaagc ctgcatggtt
     2881 tgggcatcgc ctgcatgaaa tccacaagca atattggcag aggaaaccag cgttaatagc
     2941 tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca ggtcaatttt cattgtgtag
     3001 ccgccacgct aattgttcga aataacgttg ttgatcttgc cgcgctttca gtgcctcttc
     3061 cagtgaacac tggacaaaat gaatcggctg accgagcgga atttgcgcca gatggtacat
     3121 atcagcctca atgatacagg caatacgcgg gtaaccaccg gtggtctgtg cgtcgttcat
     3181 caacacaatc ggctgcccgt tatgcggcac ctgcaccaca cccggtaaca aaccgtgaga
     3241 taacagttcg cgatcggtgg tgcgttttaa aatttgcccc tgtaagcgat agcccatgcg
     3301 gttactttgc gggctaagct gccagggcga acgccagaat gcatcctgcg aggcgcgatc
     3361 gaactcatga tattccggcc ccggcaaggc gcgaatgcgg ttgccccaca gcagctgttt
     3421 aacgccctgc gcttccataa aatcacgctt cgatttgcca atcgggagtc ggtcaccatc
     3481 cttcagtaaa cggccttcca gcccgccaat ccccacgttg agatcggtgc tgcatgaccc
     3541 cattaccggc ggaacatcaa taccacccgc gaccgccaga taactgcgca tgccgtgctg
     3601 cgggcgttta agcgttaaac gctggcctgc tttcatcggc aagcgccagc cggtccagac
     3661 ggcgttatca tccagccgcg cttcgcaacc ggcacccgtc agagcaaacc acccatcagt
     3721 ttcgaactca accgttaact gaccgagcgt gatctccagc gcgggggcat tggcgtcatt
     3781 acccaccagc aggttagcaa tgcgtaacgc aggcatatcc agtgcgccgc agtggctgat
     3841 acccgactgg cgaaaaccgt gacgaccgcc atcctgcaca gtggtataca tgcccgcacg
     3901 aataatcttc agcatactcc ctccttctgc ggtacaaagc gcacgctgtc tcccggacgt
     3961 aataagatgg gttcgtcacg cgccggatca aacaggctga gtgaggtatg accaatcaac
     4021 tgccagccac ccggcgttgc cagcggataa acaccagtct gcggcccgcc gatcccgaca
     4081 gaacctgccg gaacgagcaa gcgcggttca gcgcgccgtg gcgtgtgtag ttgttccggc
     4141 aaactcccga gatacgggaa gcccggttga aaacctaaaa accagaccac gtattccacg
     4201 gaggagtgca attcaacaac ctgtttttcg ctcaacccgc aatgcgccgc gaccaccgcc
     4261 aaatccggtc cgcctgcacc accgtaaacc accggaattt caataaagcg agactcaggt
     4321 tccagggcct cgctctcctc ccaccagcgt tgcaaaagct ctatggcatc cagcgccagc
     4381 gactcaggat tacgcaaaat caccgtgata ttgttcatgc cgggaatggc ttcaaccaca
     4441 ttcggcatat ccaccagacg ctgcgccagt cgccagatcc gtttctggct agccagcgtc
     4501 accggcggtt ccagttccag cactaccgcc gtttcaccta tcagataaca acgcgctcgt
     4561 tgcactttcg atccctctga ttattcgtta tgcaggatta ggaatatcaa taaaggtcac
     4621 atcaagatcg gtattttcat tcagccactc gctcaatgcg cgaataccac cacgttcagt
     4681 ggcatggtga cctgcagcat aaaaatgcaa tccctgctcg cgggctgaat gaatggtctg
     4741 ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa cgcgcggcgc tatcgataaa
     4801 actttgcccg ccgcccgtgc accaggcgac gcgctggacc acctcaggtc cggtatcgcc
     4861 acaccataac ggcttacgtc ccagacgcgc ttcaatccag gaggctagtt ccagtcccgg
     4921 cacaggcatg gtcagctctc cccacggcac caacggctca atttcgccca tgaccgtgat
     4981 ccccagtaac gccgccagct gtgcgttatt gcccagctca ggatgtgcgt caagcggcaa
     5041 atgccagcca tacaggttga tatcattcgc cagcaacgtt tttaaacggt tacgcttcat
     5101 tccgcgaatg accggagact ctcctttcca gaagtagcca tgatgcacaa tgaccgcatc
     5161 cgcgcccaga cgcactgcct catcgagcaa agcctggctg gcggtgacac cggtaacaat
     5221 tttttgcacc gtctctttgc cttccacctg caaaccattc ggcgcgtaat cactaatcgc
     5281 cgcgctgttc agtttttcgt taatcagttg ttccagttcg gtgtttttca tcttcgcgcc
     5341 ttccgtattc tgatagctac accgttaaca tagcgttacc tcgcctgttc gtcgatagcc
     5401 aatcttttga catattaaga aacaaacccg acgccaaaag acgcaaattg tttatatcat
     5461 cacgcttcag ctaatgtata tttatgcaat cagacagcat attgcataaa taatcagatc
     5521 tgtatcaaag ttttaacttt aaggaaacac aagcacgccg cgcaactatt acgtgacgtt
     5581 gccgtcaatt gtggaattat tatgaataaa cacgcatcac agccgcgcgc tatttactat
     5641 gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg gcatgcgtgc cctgctgatt
     5701 ctctatctca ccaatcaact aaaatacaac gatactcacg cctacgagtt atttagcgcc
     5761 tactgttcgc tggtgtatgt cacgccaatc ctcggtggct ttttggcgga taaagttctc
     5821 ggcaatcgca tggcggtgat gctgggggcg ttattgatgg cgatcggtca tgtggtgttg
     5881 ggtgccagtg agatccatcc gtcattcctc tatctgtccc tggcgataat tgtttgtggc
     5941 tatggtctgt ttaaatccaa tgtcagttgc ctgctcggcg agctgtatga gccaaccgat
     6001 ccgcgtcgtg atggcggttt ctcgctgatg tatgcagcgg gtaacgtggg gtctattatc
     6061 gcacctattg cctgtggcta cgcccaggaa gagtacagct gggcgatggg ctttggcctg
     6121 gcggcggtgg gtatgatcgc gggtctggtc attttcttat gtggcaatcg tcatttcaca
     6181 catacccgcg gcgttaataa aaaagtactg cgtgcgacaa actttctcct gcctaactgg
     6241 ggatggctgc tggttctgct ggtggcaacg cctgcgctga ttaccgtatt gttctggaaa
     6301 gagtggtcgg tatacgcctt aattgtcgcg actattattg gcctgggtgt actggcaaaa
     6361 atttatcgca aagcagaaaa ccagaaacag cgtaaggagc tggggctgat tgtgacgctc
     6421 accttcttca gtatgttgtt ctgggccttc gcacaacagg gcggcagctc gattagcctt
     6481 tatatcgacc gcttcgttaa ccgcgatatg tttggttata ccgttccgac tgctatgttc
     6541 cagtcgatta atgccttcgc agttatgctg tgcggtgtgt tcctggcgtg ggtggttaaa
     6601 gaaagcgtcg caggtaatcg taccgtgcgc atctggggaa aatttgctct tggtctcggc
     6661 ctgatgagcg ccgggttctg cattctgacc ttaagcgccc gctggtccgc aatgtatggt
     6721 cactcttctc tgccactgat ggtattaggc ctggcggtga tgggctttgc ggaactgttt
     6781 atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa tccccggtgt gaccggcgta
     6841 ttaaccggca tttatatgct gctttccggc gcgattgcga actatctcgc gggcgtgatt
     6901 gccgatcaga catcgcaggc ttcgtttgat gcttccgggg cgatcaacta ctccatcaat
     6961 gcatatattg aagtatttga tcaaattacc tggggcgcac tggcgtgtgt aggactggta
     7021 ctgatgattt ggctgtatca ggcgctgaaa ttcagaaacc gcgcgctggc gctggagtca
     7081 taataggcca gcaccatacc tgataaaacg ctcgctgcgt tttatcaggc tcctttcctc
     7141 gcttcttcgt acgctgccag cgttcgcagc cttgcctctt tgtgatcaac tatcggttgc
     7201 ggataatcca gcatcacacc tgctttctgc gcccacttcc acggctcatg caccgctttc
     7261 cctggtacat cgcgcagctc cggtaaccat cgacgaataa actcgccctc acggtcaaat
     7321 ttctcgccct gggttgtcgg gttgaaaata cgaaaatacg gcgctgcatc ggttccggtt
     7381 gaagcggccc actgccagcc accgttattg gctgccaaat caccatcaat cagctgcgac
     7441 atgaaatatc gctcgccttc gcgccagtcg atcaacaaat ctttcaccag aaaactggct
     7501 gtaatcatcc gtagccggtt atgcatccag ccagtgctgt taagctgacg catggcagca
     7561 tcgacaatcg ggtatcccgt tttgccttcc tgccaggcct gtaaatgtgc gggattgctc
     7621 tgccactgca cacgatccgt ccaggcaata aacggacaat gtttacacaa cgaggggtaa
     7681 tacgtcatca gatgacggta gaactcgcgc cagatcagct cactaagcca gacactaccg
     7741 gccccaccgt ccagcgcctg cggctgttcc gccaacaagc gatgcaaaca ctggcgaggc
     7801 gataacccgc ccgttgccag gctggcggac aaacgactgg tgccttccac tgccggaaaa
     7861 tctcgttgtt gctcatattc tccggcaccg ttctggcaaa actggcgtaa ttgcgcaatc
     7921 gccgcttttt cttccaccgg gaaatgcgca gtatcgaaag actgacgagg ataattcagc
     7981 gtaatggatg gcgagggctc tatcgatccg ctactacgaa cttttggcgc agcgacgcac
     8041 tccggcatcc cttcccgcag ccgtttcagc caggcattct taaaaggcgt aaagactttg
     8101 tacatctcgt gattaccggt catcaccgcg ccaggcggca ggatcacgct gtcatcaaat
     8161 ccttcacaca ccacgttacg cagcgctctt tcaacttcca catcccgcgc ccgctcattc
     8221 acttcatact gatagttata aaacaggtgg gtaacgctgt tttccgcgca cacctgttta
     8281 actatttcga cactggcgac aaagtcatcc acttcacgga acaataaagg aatacctttt
     8341 tccgcaagcg ctatttgtag cccattcagt tgagcattga taagttcagc ctgacgcggc
     8401 gacatgttat gcgtcgccca ctggcgtggt gtagcgatat acaacgccag cacgcgtgca
     8461 gacgaattgc tgcaggcggc agccagtgcg agattatcgt gcaaacgtaa atcctggcga
     8521 aaccagacca gatgggtagt cataaaactc ctgaatggcg caagttaatc acacgcggcc
     8581 aaagttcgaa ataacgctgc cctgaaagcc aggcgtcagg ataaagcgcc atatagtgtt
     8641 tgatacgcat cagcggcgca agaagtggtt gctcgccacg gcgataactg tcgataagcg
     8701 cagccagctg ctggcgttct gtggaatcaa tatgcgggcg aaaataaccc tgaacgtgca
     8761 tcaaaacatt ggtgtgatcg cgcgggttgg cggggtgaga aagcaaaaca actacgcgtt
     8821 ggcggtactg gttgtaatag tcatccagat tttgccactg gtgaatatca gccacgaacg
     8881 ggccaagttt gcggtactcc ggctgagaat gcgccagaaa gacgagttta tagcggctgt
     8941 gataatcgag aagcgccccg cgcgttaagc catgttcttt cagtcggtgt aactcatgaa
     9001 gggcaaagat acggattagg gtgctgtcat caaatcgttg tagattcatt actgctcctg
     9061 aacggtataa aggagtaagt gtagacgatg gtaaatgaat tttatggggc agagtttttg
     9121 cggggaagta ttttgttggt aaaaatatta tcaacttcat ctgagcgaga attattctac
     9181 tatttcccga gctgcattca ttttttgtat tttttatttt tagaaaataa cagttctcaa
     9241 agcttaatta cctcactatt aattgtattt caaactaaca atcgcactcc attttctctc
     9301 tttaaaaaac ataaacactg ttaaaacaaa tatttatttc gttatcttaa atgctatttc
     9361 tacatcactc gtccttataa tcaaattatt cttattcata gctatttttg caggtttaaa
     9421 atatcggctt tcaccaaagt cagccatgaa aattagacta caagttctcc cagcctttgc
     9481 acatgagaaa aaccacccat caactctatt tattcctgaa cctttaacac cataataaat
     9541 cagaatttct gggtattgtt catccttcac gttttcatat ttttggtatt taattgaaaa
     9601 ccaagagtct atactgccaa catcttcaaa aaatattgga gctgatgttt ccgcatgaac
     9661 atatgcagaa aatgctaaca aaatggcaat acttagtttg gtaaaaatat cgttccacac
     9721 ttttcaggat aatctattgg ggggaaggga tcttttcctc taaatctaat tgctcccact
     9781 ccttgtttgt cagaccattc gccatttgca tcctgacgat accagtgata atcccctccc
     9841 atgcctataa aatt
//
LOCUS       DAJTSL010000152         9806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTSL010000152 DAJTSL010000000
VERSION     DAJTSL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.152"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OO433_003351"
     CDS             complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OO433_003351"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6051708.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OO433_003352"
     CDS             complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OO433_003352"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6051709.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSIFNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2696..3387)
                     /locus_tag="OO433_003353"
                     /pseudo
     CDS             complement(2696..3387)
                     /locus_tag="OO433_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3517..5277
                     /locus_tag="OO433_003354"
     CDS             3517..5277
                     /locus_tag="OO433_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6051710.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5683..6540
                     /gene="focA"
                     /locus_tag="OO433_003355"
     CDS             5683..6540
                     /gene="focA"
                     /locus_tag="OO433_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6051711.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6595..8877
                     /gene="pflB"
                     /locus_tag="OO433_003356"
     CDS             6595..8877
                     /gene="pflB"
                     /locus_tag="OO433_003356"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6051712.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9069..>9806
                     /gene="pflA"
                     /locus_tag="OO433_003357"
     CDS             9069..>9806
                     /gene="pflA"
                     /locus_tag="OO433_003357"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6051713.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2516 a         2468 c         2342 g         2480 t
ORIGIN      
        1 atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc
       61 tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt
      121 gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat
      181 ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg
      241 aatgtaatcg tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc
      301 aaacaggcga tcggtttctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc
      361 ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc
      421 catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat
      481 ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg
      541 tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga
      601 agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag actgtccgcc
      661 ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag
      721 attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt
      781 atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga
      841 gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt
      901 ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc
      961 aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca
     1021 aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc
     1081 accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga
     1141 aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct
     1201 atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg
     1261 gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg
     1321 taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg
     1381 gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga
     1441 agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc
     1501 cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga
     1561 acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag
     1621 ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg
     1681 ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa
     1741 agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat
     1801 cgttgaagat ggaataatcc agtatcgacg gacacgcgat attcgctttg cccagcaaat
     1861 cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt
     1921 aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg
     1981 cggcgaccac cacatctttg ccgaagtctg gcgtctcgtc gatggcgata ccgtcgatgg
     2041 tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg
     2101 gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt
     2161 attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg
     2221 tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat
     2281 ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg
     2341 cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac
     2401 caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca
     2461 ttgccggacc agaactaaaa ttgaagattt gagccatttc ccctcaccac gttgcgttgc
     2521 tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg
     2581 ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgaggct
     2641 tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg
     2701 tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt
     2761 gttttccccc gctttcatat gaatgatttc actacaacga atacaggcat agtccgccga
     2821 ctccggcacc actttgtaac gctgcgtaca atcactgggt aaaataagta aacccggttt
     2881 caccttgtca tcttcaaaga aatagacact gatttgcccg ttggtttcga gaatcgccag
     2941 acgtacctgc cccagctgct ccacgccacg caatcgtagc tccataaaga actcaaattc
     3001 cgtcatgttg gagttattga gtttcgacca ggccagctcg ccatcttcaa taataacaac
     3061 cggcttacct tccagtagat cttccagttt ttcactgtgc gccatcaacc acattaccag
     3121 gcggtataac aacgccagag tgataaagac gataagtacc ggcaccatcg gcacatcatc
     3181 ataaaacgcc acatctcccg ccgctgaacc cagcgtgaga atgattaaca cttcaaacag
     3241 cgacatctgt cgcacaccgc gtcttccggt cattttgagg aacaaaaaaa ccagtacaaa
     3301 ggtataaaga ctacgtagtg caacttcgcc aaggaaatca aaaggcactt tatcaaatgc
     3361 catacggtgg agatcgaacg ccttcatttt tatgctctta tcgtggttta ttcgctaaaa
     3421 gcatagtcaa cagttcatcg gcatggcggc ggtgatagcg cctttgagat aaaacccgta
     3481 ttattgcgcg ctttccgtac gactaaagtg attttcatga cgcaaacatt tatccccggc
     3541 aaagatgccg ctctggaaga ttccatcgct cgcttccagc aaaaactttc agacctcggc
     3601 tttcagattg aagaggcctc ctggctgaat cctgtgccta acgtctggtc tgtacatatt
     3661 cgcgacaaag agtgcgcact gtgttttacc aacggtaaag gcgcaaccaa aaaagcggcg
     3721 ctggcttctg cacttggtga atatttcgag cgtctctcaa ccaactactt ttttgctgat
     3781 ttctggctgg gcgaaaccat cgccaacggt ccgttcgtgc attatcccaa cgaaaaatgg
     3841 ttcccactga ccgaaaatga cgatgtgcca gaaggactac tcgatgaccg tctgcgcgcg
     3901 ttttatgatc cggagaatga actgaccggc agcatgctga ttgacctaca atccggtaac
     3961 gaagatcgtg gtatttgcgg cctgccgttt acgcgtcagt ccgacaatca gaccgtttat
     4021 attccgatga atatcattgg taacctgtac gtctccaacg gtatgtccgc aggtaatacc
     4081 cgcaacgaag cacgcgttca gggattgtct gaagttttcg aacgctacgt gaaaaaccgc
     4141 attattgctg aaagcatcag cctgccagag atcccggcag acgtgctggc gcgttaccca
     4201 gcggtggttg aagcgatcga aacactggaa gcagaaggtt tcccgatctt cgcatatgat
     4261 ggttcccttg gcggccagta tccggtgatt tgcgtggtac tgttcaatcc tgctaacggt
     4321 acctgctttg cctctttcgg tgcgcatcct gattttggcg tagcactgga acgtaccgtg
     4381 accgagctgc tgcaaggtcg tggcctgaaa gatttggatg tgtttactcc gccaaccttc
     4441 gatgatgaag aagtcgctga acataccaac ctcgaaacgc actttatcga ttccagcggt
     4501 ttaatctcct gggacctgtt caagcaggat gccgattatc cgtttgtgga ctggaatttc
     4561 tccggcacca cggaagaaga gtttgctacg ctgatggcta tcttcaacaa agaagataaa
     4621 gaagtttata ttgccgatta cgagcatctg ggcgtttatg cttgccgtat tatcgtgcct
     4681 ggcatgtccg atatttatcc ggctgaagat ctgtggctcg cgaataacag tatgggcagc
     4741 catttacgtg aaacgattct ttcgctacca ggcagcgagt gggaaaaaga agattacctg
     4801 aacctcatcg agcaactgga tgaagaaggt tttgatgact ttacccgcgt gcgtgagctg
     4861 ttgggtctgg cgaccgggtc ggataacggt tggtacaccc tgcgtatcgg tgaattaaaa
     4921 gccatgctgg cgctggctgg tggcgatctg gaacaggctc tggtctggac cgaatggacg
     4981 atggagttta actcatcagt atttagtccg gaacgcgcca actattatcg ctgcctgcaa
     5041 acgttgttat tactggcaca ggaagaagat cgccagccgc tgcaatatct gaatgcgttt
     5101 gttcgcatgt acggcgcaga tgccgtggaa gccgccagtg cggcaatgag cggcgaagcg
     5161 gcgttttacg gcctgcaacc ggtagatagc gatctgcacg cgtttgctgc acatcagtcg
     5221 ttgttgaagg cctacgaaaa gctgcagcgc gccaaagcag cattctgggc aaaataaaat
     5281 caaatagcct acgcaatgta ggcttaatga ttagtctgag ttatattacg gggcgttttt
     5341 ttaatgcccc gctttacata tatttgcatt aataaaataa ttgtaattat aaggttaaat
     5401 atcggtaatt tgtatttaat aaatacgatc gatattgtta ctttattcgc ctaatgctcc
     5461 cttttaatta actattttag cggaggatgc ggaaaaaatt caactcattt gttaattttt
     5521 aaaatttatt tttatttgga taatcaaata tttactccgt atttgcataa aaaccatgcg
     5581 agttacggac ctataagcca ggcgagatat gatctatatc aatttctcat ctataatgct
     5641 ttgttagtat ctcgtcgccg acttaataaa gagagagtta gtgtgaaagc tgacaaccct
     5701 tttgatcttt tacttcctgc tgcaatggcc aaagtggccg aagaggcggg tgtctataaa
     5761 gcaacgaaac atccgcttaa gactttctat ctggcgatta ccgccggtgt tttcatctca
     5821 atcgcattcg tcttctatat cacagcaacc actggcacag gcacaatgcc cttcggcatg
     5881 gcaaaactgg ttggcggcat ttgcttctct ctggggctga ttctttgtgt tgtctgcgga
     5941 gctgatctct ttacttccac cgtgttgatt gttgttgcta aggcgagtgg gcgcatcacc
     6001 tggggtcagt tggcgaaaaa ctggctaaat gtctattttg gcaacctggt cggcgcactg
     6061 ctgtttgtac ttttaatgtg gctttccggc gagtatatga ccgcaaatgg tcaatgggga
     6121 ctaaacgtcc tacaaaccgc cgaccacaaa gtgcaccata cttttattga ggccgtctgt
     6181 cttggtatcc tggcaaacct gatggtatgt ctggcagtat ggatgagtta ttctggccgc
     6241 agcctgatgg acaaagcgtt cattatggtg ctgccggtcg cgatgtttgt tgccagcggt
     6301 tttgagcaca gtatcgcaaa catgtttatg atcccgatgg gtattgtaat ccgcgacttc
     6361 gcatctccgg aattctggac cgctgtcggt tctgcaccgg aaaatttttc tcacctgacc
     6421 gtgatgaact tcatcactga taacctgatc ccggttacga tcggtaatat tatcggcggt
     6481 ggtttgttgg ttgggttgac atactgggtc atttacctgc gtgaaaacga tcaccattaa
     6541 tggttgtcga agtacgcagt aaataaaaaa tccacttaag aaggtaggtg ttacatgtcc
     6601 gagcttaatg aaaagttagc cacagcctgg gaaggtttta ccaaaggtga ctggcagaat
     6661 gaagtaaacg tccgtgactt cattcagaaa aactacactc cgtacgaggg tgacgagtcc
     6721 ttcctagctg gcgctactga agcgaccacc accctgtggg acaaagtaat ggaaggcgtt
     6781 aaactggaaa accgcactca cgcgccagtt gactttgaca ccgctgttgc ttccaccatc
     6841 acctctcacg acgctggcta catcaacaag cagcttgaga aaatcgttgg tctgcagact
     6901 gaagctccgc tgaaacgtgc tcttatcccg ttcggtggta tcaaaatgat cgaaggttcc
     6961 tgcaaagcgt acaaccgcga actggatccg atgatcaaaa aaatcttcac tgaataccgt
     7021 aaaactcaca accagggcgt gttcgacgtt tacactccgg acatcctgcg ttgccgtaaa
     7081 tctggtgttc tgaccggtct gccagatgca tatggccgtg gccgtatcat cggtgactac
     7141 cgtcgcgttg cgctgtacgg tatcgactac ctgatgaaag acaaactggc acagttcact
     7201 tctctgcagg ctgatctgga aaacggcgta aacctggaac agactatccg tctgcgcgaa
     7261 gaaatcgctg aacagcaccg cgctctgggt cagatgaaag aaatggctgc gaaatacggc
     7321 tacgacatct ctggtccggc taccaacgct caggaagcta tccagtggac ttacttcggc
     7381 tacctggctg ctgttaagtc tcagaacggt gctgcaatgt ccttcggtcg tacctccacc
     7441 ttcctggatg tgtacatcga acgtgacctg aaagctggca agatcaccga acaagaagcg
     7501 caggaaatgg ttgaccacct ggtcatgaaa ctgcgtatgg ttcgcttcct gcgtactccg
     7561 gaatacgatg aactgttctc tggcgacccg atctgggcaa ccgaatctat cggtggtatg
     7621 ggcctcgacg gtcgtaccct ggttaccaaa aacagcttcc gtttcctgaa caccctgtac
     7681 accatgggtc cgtctccgga accgaacatg accattctgt ggtctgaaaa actgccgctg
     7741 aacttcaaga aattcgccgc taaagtgtcc atcgacacct cttctctgca gtatgagaac
     7801 gatgacctga tgcgtccgga cttcaacaac gatgactatg ctatcgcttg ctgcgtaagc
     7861 ccgatgatcg ttggtaaaca aatgcagttc ttcggtgcgc gtgcaaacct ggcgaaaacc
     7921 atgctgtacg caatcaacgg cggcgttgac gaaaaactga aaatgcaggt tggtccgaag
     7981 tctgaaccga tcaaaggcga tctcctgaac tacgatgaag tgatggagcg catggatcac
     8041 ttcatggact ggctggctaa acagtacatc actgcactga acatcatcca ctacatgcac
     8101 gacaagtaca gctacgaagc ctctctgatg gcgctgcacg accgtgacgt tatccgcacc
     8161 atggcgtgtg gtatcgctgg tctgtccgtt gctgctgact ccctgtctgc aatcaaatat
     8221 gcgaaagtta aaccgattcg tgacgaagac ggtctggcta tcgacttcga aatcgaaggc
     8281 gaatacccgc agtttggtaa caatgatccg cgtgtagatg acctggctgt tgacctggta
     8341 gaacgtttca tgaagaaaat tcagaaactg cacacctacc gtgacgctat cccgactcag
     8401 tctgttctga ccatcacttc taacgttgtg tatggtaaga aaactggtaa caccccagac
     8461 ggtcgtcgtg ctggcgcgcc gttcggaccg ggtgctaacc cgatgcacgg tcgtgaccag
     8521 aaaggtgctg tagcgtctct gacttccgtt gctaaactgc cgtttgctta cgctaaagat
     8581 ggtatctcct acaccttctc tatcgttccg aacgcactgg gtaaagacga cgaagttcgt
     8641 aagaccaacc tggctggtct gatggatggt tacttccacc acgaagcatc catcgaaggt
     8701 ggtcagcacc tgaacgttaa cgtgatgaac cgtgaaatgc tgctcgacgc gatggaaaac
     8761 ccggaaaaat atccgcagct gaccatccgt gtatctggct acgcagtacg tttcaactcg
     8821 ctgactaaag aacagcagca ggacgttatt actcgtacct tcactcaatc tatgtaatta
     8881 gatttgactg aaatcgtaca gtaaaaagcg tacaataaag gctccacgaa agtggggcct
     8941 tttttagcac gagagccttt tttgtcagct atctatactt taaggtgact gccaaaacag
     9001 actcgacgta gccttcgagc tgcgcaccaa cacggcctca gatgggccac atctggagaa
     9061 ataccgcaat gtcagttatt ggtcgcattc actcctttga atcctgtgga accgtagacg
     9121 gcccgggtat tcgctttatc acctttttcc agggctgcct gatgcgctgc ctgtattgtc
     9181 ataaccgcga cacctgggac acgcatggcg gtaaagaagt taccgttgaa gatttgatga
     9241 aggaagtggt gacctatcgc cactttatga acgcttccgg cggcggcgtt accgcatccg
     9301 gcggtgaagc aatcctgcaa gctgagtttg ttcgtgactg gttccgcgcc tgcaaaaaag
     9361 aaggcattca tacctgtctg gacaccaacg gttttgttcg tcgttacgat ccggtgattg
     9421 atgaactgct ggaagtaacc gacctggtaa tgctcgatct caaacagatg aacgacgaga
     9481 tccaccaaaa tctggttgga gtttccaacc accgcacgct ggagttcgcc aaatatctgg
     9541 cgaacaaaaa tgtgaaggtg tggatccgct acgttgttgt cccaggctgg tctgacgatg
     9601 acgattcagc gcatcgcctc ggtgaattta cccgtgatat gggcaacgtt gagaaaatcg
     9661 aactcctccc ctaccacgag ctgggcaaac acaaatgggt ggcaatgggt gaagagtaca
     9721 aactcgacgg tgttaaacca cctaagaaag agaccatgga acgcgtgaaa ggcattcttg
     9781 agcagtacgg tcataaggta atgttc
//
LOCUS       DAJTSL010000153         9785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTSL010000153 DAJTSL010000000
VERSION     DAJTSL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.153"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            114..734
                     /gene="fklB"
                     /locus_tag="OO433_003358"
     CDS             114..734
                     /gene="fklB"
                     /locus_tag="OO433_003358"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6051714.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1043..2455
                     /gene="cycA"
                     /locus_tag="OO433_003359"
                     /gene_synonym="dagA"
     CDS             1043..2455
                     /gene="cycA"
                     /locus_tag="OO433_003359"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6051715.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFLG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(2500..3162)
                     /gene="ytfE"
                     /locus_tag="OO433_003360"
     CDS             complement(2500..3162)
                     /gene="ytfE"
                     /locus_tag="OO433_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6051716.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYNMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(3270..4235)
                     /locus_tag="OO433_003361"
     CDS             complement(3270..4235)
                     /locus_tag="OO433_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6051717.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIVVRAKPEKPLTESVSES"
     gene            complement(4343..5203)
                     /gene="qorB"
                     /locus_tag="OO433_003362"
     CDS             complement(4343..5203)
                     /gene="qorB"
                     /locus_tag="OO433_003362"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6051718.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLTDSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            5292..5672
                     /locus_tag="OO433_003363"
     CDS             5292..5672
                     /locus_tag="OO433_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6051719.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(5801..7744)
                     /gene="cpdB"
                     /locus_tag="OO433_003364"
     CDS             complement(5801..7744)
                     /gene="cpdB"
                     /locus_tag="OO433_003364"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6051720.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIIGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEEADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDRHIAFASPDE
                     NRSVLTAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            7934..8674
                     /gene="cysQ"
                     /locus_tag="OO433_003365"
     CDS             7934..8674
                     /gene="cysQ"
                     /locus_tag="OO433_003365"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6051721.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            8886..>9785
                     /locus_tag="OO433_003366"
     CDS             8886..>9785
                     /locus_tag="OO433_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051722.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKKEQLA"
BASE COUNT         2389 a         2359 c         2502 g         2535 t
ORIGIN      
        1 gctattgttt ctttttcccc gggatttgtc atcattcctg tgacaaagtt ttacccaaag
       61 aagtgtggct gatatgctgc ccgctacttt tataccctaa gaaaggaaat acgatgacca
      121 ccccaacttt tgacactatc gaagcgcaag caagctacgg cattggtttg caggtagggc
      181 aacaactgag tgaatctggc ctggaagggc tgctgccaga agcactggtt gcaggtattg
      241 ccgatgcgct ggaaggcaaa catccggctg ttccggttga tgtggtgcat cgcgcgctgc
      301 gtgaaatcca cgaacgcgcc gatgccgttc gtcgtcagcg tttccaggcg atggctgctg
      361 aaggtgtgaa atacctggaa gaaaacgcca aaaaagaagg tgtgaatagc accgaatctg
      421 gcctgcaatt ccgcgtgatc aaccagggtg aaggcgcaat tccggcacgt accgaccgcg
      481 ttcgtgttca ttacaccggt aaactgatcg acggcaccgt gtttgacagc tccgttgctc
      541 gtggtgaacc cgctgaattc ccggttaacg gcgtgatccc tggctggatt gaagcactga
      601 ctctgatgcc ggtaggttct aaatgggaac tgactatccc gcaggaactg gcatatggcg
      661 agcgcggcgc aggtgcatcc atccctccgt tcagcaccct ggtgtttgaa gtcgaactgc
      721 tggaaatcct ctaagcagcg cattctgttc ccctcgaacc agaggggagc aggcattcag
      781 caataaaccc ttcagtttgc caaacggcgc tattttgtgt tgcaaagacc ccgtaagcgt
      841 gtatttttgt gagatgtttc gcgttatcac cgtgatatga cactcacttt aaacataaaa
      901 ttaacattag atctaaatct tagtattcat cccgcgtatt gttacctaat atcgatgagt
      961 cccgatacag attcgtcgta tcatagactg actaaaggcc gtagagcctg aacaacacag
     1021 acaggtacag gaagaaaaaa acatggtaga tcaggtaaaa gtcgttgccg atgatcaggc
     1081 tccggctgaa cagtcgctac ggcgcaatct cacaaaccga catattcagc ttattgccat
     1141 tggcggtgcc attggtacgg ggttgtttct ggggtctggc aaaacgatta gccttgccgg
     1201 gccgtcgatc attttcgttt atatgatcat tggttttatg ctctttttcg tgatgcgggc
     1261 aatgggggaa ttgctgcttt cgaatctgga atacaaatct tttagtgact tcgcttccga
     1321 tttactcggg ccgtgggcag gatatttcac tggctggact tactggttct gctgggttgt
     1381 aaccggtatg gcagacgtgg tggcgatcac ggcttatgct cagttctggt tccccgatct
     1441 ctccgactgg gtcgcctcgc tggcggtgat agtgctgctg ctgacgctca accttgccac
     1501 cgtgaaaatg ttcggtgaga tggagttctg gtttgcgatg atcaaaatcg tcgccatcgt
     1561 gtcgctgatt gtcgtcggcc tggtcatggt ggcgatgcac tttcagtcac cgactggtgt
     1621 ggaagcgtca ttcgcgcatt tgtggaatga cggcggctgg ttcccgaaag gtttaagtgg
     1681 cttctttgcc ggattccaga tagcggtttt cgctttcgtg gggattgagc tggtaggtac
     1741 aacagctgcg gaaaccaaag atccggagaa atcactgcca cgcgcgatta actccattcc
     1801 gatccgtatc attatgttct acgtcttcgc gctgattgtg attatgtccg tgacaccgtg
     1861 gagttcggta gtcccggaga aaagcccgtt cgttgagctg tttgtgttgg taggtttgcc
     1921 tgcggctgcc agcgtgatca actttgtggt gctgacctct gcggcgtctt ccgctaacag
     1981 cggcgtcttc tctaccagcc gtatgctgtt tggtctggcg caggaaggtg tggcaccgaa
     2041 agcgttcgct aaactttcta agcgcgcagt accagcgaaa gggctgacct tctcctgcat
     2101 ctgcctgctc ggtggcgtgg tgatgctgta tgtgaatcct agcgtgattg gtgcgttcac
     2161 gatgattaca accgtttccg cgattctgtt tatgttcgtc tggacgatta tcctttgctc
     2221 gtacctggtg taccgcaaac agcgtcctca tctgcatgag aagtcgatct acaagatgcc
     2281 gctcggcaag ctgatgtgct gggtatgtat ggcgttcttt gtgttcgtgg tcgtgttgct
     2341 gacactggaa gatgacactc gccaggcgct gctggttacc ccgctgtggt ttatcgcact
     2401 ggggctgggc tggttgttta ttggtaagaa gcgggctgct gaactgcgga aataaccgca
     2461 ttatcatgct ggatggcgca atgccatcca gcttttagat cactcacccg ccagcgcgcg
     2521 tgggaacagt acattgtttt ccagactgat gtgatccatc aggtcatcaa tcagttcatt
     2581 aatgccgtta tacatcgctt tccaggtggt gcaggcttct ggcggcggtg tgacgttatt
     2641 ggtagtgtgt ttgatcactt ccagcagttc gcccgcttca tcgtgctcgc tttccattac
     2701 gctgattggc cccattgcct ggctgcccat gccttgtttg atcatcggga agaggatctg
     2761 ctcttctttc atcatgtggc tggaaagctc ttcatgcagc atggtcaggt attttgtcag
     2821 cccttttggc acgctcggtt tgtcggcgtg aacgcgctcg actttagtcg cttgcaggat
     2881 cagctccggc agttgctcgc ggtgacgatc gtggtagcgc acgatgatat ggtcaatgat
     2941 ttctgccagc ggggcgctac gccagtcttt ctcaatcggt tgttcagcaa gctttgccag
     3001 ttcagcttca atgacctcaa catccagttc tttacgtgcc gccgcgcgcg ccagcgtctg
     3061 cttaccgcca cagcagtaat ccatattata tttacgaaac agagctgaag cgcgaggaat
     3121 agagagcgcc agttcaccta aaggttggtc gcgataagcc atagctgata cctcattctt
     3181 aataagataa gatgtatttt aaatacatct ttaaggcaaa agctataacc cttacgtagc
     3241 agaggcttat ttaactcaca gccagtatgt caactttctg agacagattc agttaaaggc
     3301 ttttccggtt ttgctctgac tacaatgacc acgccaacca ctaacagcgc gataccgctc
     3361 agcgtcatca gcggcggcat ttgctggcgt atcaaaaagg tgtacagcaa acctgccagt
     3421 gtttcgaaaa cgatcagcgg cccgagaatc actgtcggta atcgctggct ggcgacgttc
     3481 cagcagagtg cgccaaccca tgagcaaagc acggctatcg caaccatcag actaataaac
     3541 accagcggac gggggccaaa aggtagggag aagtccggcg tttgcgtatt cagccagtaa
     3601 caggcgacga gatagccgat gagagaaacc ggcagtgtga ccagcgcctg cgccgtcgcc
     3661 cacatcatcg gatgtttgtc gggattctcc cgcagccagc gggcgttgcg cagggcatac
     3721 catgcccagc agaccacgga aactaacgct agcacgatgc ctgaggtata acgtgcccag
     3781 tcaaaatcgg ggagtccgtg gtttaactca gcaatattca cacacgccag gccgatgcca
     3841 atacaaacca gtgccggggc gagttttccc cacgcgagtt tgccgtcgcg ctggctataa
     3901 agcagattgg caaaaacggg aatcaccacc ggcagggtgc cgataatcat cgtggaaaca
     3961 ggcgcaccag tacgttgaat ggcactggca aggcagaagt aatagatgag gttgcccatc
     4021 atagtgagca tcaaggcggt aagccagtcg cgacgcgcca actgacgcag acgcacgcgt
     4081 cccagccagg caatgggcag cgcaattaac cctaacgcca gataacgccc catcgactgc
     4141 aacatcgccg ggtattccgg tacgatcaac ggcccgacaa aaataagccc ccacatcaac
     4201 cctgctaaca gggcgtacag cacgccgcta atcattactg gcatccattg gtctgtcaga
     4261 agaaagttca ggaagcagac atgattgctc ccggcatccc gtcattatta gagaggatgc
     4321 gggaggatgc cactttaatt aactagttat taacattaaa aagatggctt acgctttcgg
     4381 ctaacgttgt cgttgggcgg ccaatcaatt tgctaagcgt tttgctgtta tcaaacagac
     4441 cacctttcga tgcgccaacg tcagaatccg tcagcatatc cgccagtccg tcgggcagtc
     4501 cgacgctttt cagtgccgcg gcgaaatcgg cttcgctcag attttgatag gtaacctgtt
     4561 tgccgctctg tttggtcagc tctgccgcta actgtgtcaa cgtccaggca ctatcgcccg
     4621 ccagttcgta aaccttgcct tcgtgaccgg cttcgctaat cacgcgtgcc gcagctgccg
     4681 cataatctgc ccgcgttgct gaggcgattt tgccatcgcc cgccgcaccg ataaatacgc
     4741 cgtgttccag tgctgccggg gcgctggcga ggtagttttc gctgtaccag ccgttgcgca
     4801 gcagggtgta aacgatgcca gaatcagcca acattttctc cgtctcaatg tgctcatcgg
     4861 cgaggccgag cggggaggta tctgcatgta gcaggctggt ataagcgata aatttcacgc
     4921 cagccgcctt tgcggcatta ataacattac gatgctgcgg ggcacgttga cccacttcgc
     4981 tggaagagat cagcagtagt ttttccactc cctgaagtgc agatgtcagt gcggcttcat
     5041 cgccgtagtc agcctgacgc acggtaatgc cttgtgctgc cagagcctgg gcttttgccg
     5101 gattacgaac gatagccact atttggctgg caggaaccgt tttcatcaag gattcaataa
     5161 tatagtgacc aagttggcca gtggcaccag taatagcgat catgggaagt ctccatcgtt
     5221 ttgtcttgtg tttaaatacg ctaacaccta aacttacttt tagtaagtac gtacaaaaag
     5281 gtaagtatga aatgagtcag gttagcctgt cgcaacaact gaaagagggt aatctctttg
     5341 cggaacagtg cccgtcgcgc gaggtgttga aacacgtcac cagccgttgg ggggtgttga
     5401 ttctggtggc gctacgcgaa ggtactcatc gctttagcga cctgcggcgc aaaatcggcg
     5461 gggtgagcga aaagatgctt gcgcagtcgt tacaggcgtt agaacaggat ggttttctta
     5521 accgtatcgc gtatccggtg gtgccaccgc atgtggaata tagcctcacg ccgttgggcg
     5581 agcaggtgag cgaaaaggtt gctgcactgg cggactggat tgagttgaat ttgcccgagg
     5641 tgttggcggt gcgggatgaa cgtgcggcat aactgccgga tgcgctgagc ttatctggtc
     5701 tacggtgcat ggctggtagg gcggataaga tgcgccagca tcgcatccgc caataaatgc
     5761 cggatgcggt tgataattta tagaccgaaa aagaagtgtt ttacttactc aaatccacct
     5821 gataaatcgc aaacccgata tcatcggtcg cgactttatt catcggatat tgccctttct
     5881 ctttaataaa cgctgcggct ttatctgacg gggaagtttc gaaacggata tccagtttct
     5941 tatcgccagc tatcggtgct aaacgccagt tgttatctgc cgccgggtga atttctcccg
     6001 cacgtttcga ctcatcagca atccacgctg tcagcaccga gcggttctca tccggtgaag
     6061 caaaagcgat atgtctgtcg cccgtaccgg caaatttgcc gccgtaagcg cgatagttat
     6121 tggtggcaac gaggaacata gcgttcggat caatcggctt gccattaaag gtcaggttct
     6181 taatccgctc tgcattggca ttaatcatct ggcactcgcc gtcatagcgg gcgggttggg
     6241 taacatcaat ctgataattc acaccatcaa taacatcaaa gttataagtg cggaaaccat
     6301 cccagttgat gagtgactgt ggtttcgtgc tgttaggatc aatctggtta aactgtccgg
     6361 cggagcactc cagccactct ttcacctctt taccgctggc tttcactaca atcagcgtat
     6421 tggggtagag ataaagatcg gcggcattac ggaaggtcaa ctggcctttt tccacctcca
     6481 catagcttgc cgggtcattt ttgcgaccac caactttaaa cggtgcggca gctgaaagca
     6541 ccggcagttt tgccagatcc ggatcgccct gaatgtaatg ttcgacatac gctttttgcg
     6601 cgttgttcac gacttgcacg gtcggatcgt cctgcaccag cgccagatag ctatacatat
     6661 tgtcggcaga tttaccgatt ggcttgctga cgaactggcg tgtggcatcg tgatcggctt
     6721 tgagtgtttc taccagcttg ctgtcttccg ccgcgaggga ttttttattg gcgatgtcgt
     6781 aaatcggccg tgcttccgct ttcgcctgcg tcacctgcca tttaccgctg tcattactga
     6841 gttgtaagtc gaccacccca agatgatcgc cccacatgcc cggcattacc gccggaacac
     6901 cattcagcgt gcctttggtg atatcagcct cttcgatatc agcaaaatct ttacccggga
     6961 aaacggcgtg agcatggcca aacataatgg cgttaacgcc cggaatttca ctgaggtaat
     7021 aaactgagtt ttccgccatt actttatacg gatcggcaga cagcccggaa tgcgccagaa
     7081 cgacaacgac atcggcacct ttctcgcgca tttcaggcac gtatttgcgc acggtttcgg
     7141 taatatcatt caccgtcact tttccggata aattagcttt atcccagcct atgatttgcg
     7201 gcggcacgac gccaatatag ccaatcttca gcgtctgttt ttttccgtct ttatcgacca
     7261 cttcggtatc tttaattaaa tacggtgtaa acattggctg tttggttctg gcgtcaatga
     7321 cgttggcatt tacataaggg aatttcgctc ccgccagcgc atttttcagg taatccagac
     7381 cgtagttaaa ttcatgattg ccgagtgtac cgaccgtata atccagcgta ttcagcgcct
     7441 tataaaccgg atgaacatca cctgctttta atcctttcgc cgacatgtaa tcggccagcg
     7501 gactcccctg aatcaaatcg ccgttatcga ccagtacgct gtttttcact tcattgcggg
     7561 catcgttaat caggcttgcc gtacgtacca gtccgaattt ttccgtggcg gtgtctttgt
     7621 aataatcgaa atccatcatg ttgctatgca gatcagtggt ttccatgata cgcagatcga
     7681 ccgtcgctgc attcacactg gcggcaatca gcgtggccag gagcgttgcg ctaaacttaa
     7741 tcatcaggga catcctttta tcatcgggaa tacgaaagaa aagggagaat aaacgtctta
     7801 cttatagaac agtgaagaat gccacaattt tacgctttga aaatgatgac actatcacag
     7861 ttggcgcatt cattaacgat agggtataag taaaacaata agttaacacc gctcacagag
     7921 acgaggtgga gaaatgttag atcaagtatg ccagcttgca cggaatgcag gcgatgccat
     7981 tatgcaggtc tacgacggga cgaaaccgat ggacgtcgtc agcaaagcgg acaattctcc
     8041 ggtaacggca gcggatattg ccgcacacac cgttatcatg gacggtttac gtacgctgac
     8101 accggatatt ccggtccttt ctgaagaaga tcctcccggt tgggaagtcc gtcagcactg
     8161 gcagcgttac tggctggtag acccgctgga tggtactaaa gagtttatta aacgcaatgg
     8221 cgaattcacc gttaacattg cgctcattga ccatggcaaa ccgattttag gtgtggtgta
     8281 tgcgccggta atgaacgtaa tgtacagcgc ggcagaaggc aaggcctgga aagaagagtg
     8341 cggtgtgcgc aagcagattc aggtccgcga tgcgcgcccg ccgctggtgg ttatcagccg
     8401 ttcccatgca gatgcggagc tgaaagagta tctgcaacag cttggcgaac atcagaccac
     8461 gtccatcggc tcttcgctga aattctgcct ggtggcggaa ggacaggcgc agctgtaccc
     8521 gcgcttcgga ccaacgaata tttgggacac cgccgctgga catgctgtgg ccgcagctgc
     8581 cggagcgcac gttcacgact ggcagggtaa accgctggat tacactccgc gcgagtcgtt
     8641 cctgaatcct gggttcagag tgtctattta ctaaattcag atggcagaaa cagtgtattt
     8701 cctgattctg ccatcctgat ttctcccaac ctaaaaagtt ataaataaaa agagattgta
     8761 tttaaagtgc aaaaattcaa ttgctaataa gttacatttt aataatgagc gttttttgat
     8821 agtttacttc tataatgaga tatttaatgg cgacataaaa taaccaaata aaataaggta
     8881 gccatatgtt acccaggatc agacacaata attttattgg tgcggtggag ttattcgtaa
     8941 aatcatcgca tacaaaaaca cattcaaaca atttttttaa taatattcag catgcattta
     9001 agaaaaaaga ttgggtttcg aattatgata gcctgttaac tttgtgggag ttttttcgtt
     9061 gcgcaactca gattgataaa gatagttatc aagtgctttc atcaaaaaat gaaactgtta
     9121 aagccatgga taagtttctt atcagtttca gccttaaaga taacggtgcc gaatatacaa
     9181 tgacattgcg aggcagtgga tttgaatacg aagaaatccc cataacaata aatgagtata
     9241 actcattcat ggatttcaaa aaccgtgaat ttccgttaga acaaaacaga agattgtatg
     9301 cttgggatat tctgcagaaa aaacagtctg acataccgaa aaggattaaa ggttatattc
     9361 accaggcgat tggtgacgtg tctttaggtt atgccttatt ggaggatata gtaagcaaat
     9421 taaaacgggg taaatttgaa ctccaaatac caggaggtgg aattaaagaa tgcgatggat
     9481 ggtatattta cgaaaaaata attgatgata attttgccat agtcatagaa tctttgggct
     9541 ttgcgcttaa aatctatgga ggggatgaac gttttcgtaa tggttcctct gttgttttgg
     9601 aggatgaaga ttactctctt atttataatt ttctggttaa tgcaggttgc caacaagtcg
     9661 aattagccga acaagtggat gcgatagtaa gtgcgaatct ggccgtagat agtgatatta
     9721 ccgaagagga gatttgcgag aaatataaat caaccattga ggcgtttaaa aaagagcaac
     9781 tagcg
//
LOCUS       DAJTSL010000154         9743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTSL010000154 DAJTSL010000000
VERSION     DAJTSL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.154"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            260..457
                     /locus_tag="OO433_003367"
     CDS             260..457
                     /locus_tag="OO433_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051723.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(407..1600)
                     /locus_tag="OO433_003368"
     CDS             complement(407..1600)
                     /locus_tag="OO433_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6051724.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     VRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1736..3460
                     /gene="iucA"
                     /locus_tag="OO433_003369"
     CDS             1736..3460
                     /gene="iucA"
                     /locus_tag="OO433_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6051725.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3461..4408
                     /gene="iucB"
                     /locus_tag="OO433_003370"
     CDS             3461..4408
                     /gene="iucB"
                     /locus_tag="OO433_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6051726.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4408..6150
                     /gene="iucC"
                     /locus_tag="OO433_003371"
     CDS             4408..6150
                     /gene="iucC"
                     /locus_tag="OO433_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6051727.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6147..7484
                     /gene="iucD"
                     /locus_tag="OO433_003372"
     CDS             6147..7484
                     /gene="iucD"
                     /locus_tag="OO433_003372"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6051728.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVITLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7490..9685
                     /gene="iutA"
                     /locus_tag="OO433_003373"
     CDS             7490..9685
                     /gene="iutA"
                     /locus_tag="OO433_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6051729.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWIIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2337 a         2516 c         2607 g         2283 t
ORIGIN      
        1 tcataaaaaa catgtcagtg aaaaagaaac ctggcgttgg gttgaaggat ataataaagc
       61 aacagaactg gctcgccaat gccctgatac ccatgtattg tcaatttctg atcgtgaagg
      121 cgatttttat gacctgtttg aacgcgcaga gcagactccc ggaataaaag ctgactggct
      181 tgtcagaatg aaatttaata acagagcaac gctgaatatt aatggtaaac gggatcatag
      241 gttgttgcac gaaagaatca tggagattac accgcagcaa ctggttgaat tcactatacc
      301 tgatgggcgg gggcagcaag ccagaacact gcctgacatg ctttcatcag acgcaggtcc
      361 tctctcatat cctctgattt actccagtaa ggtgatgatc atatctttat ttgatagtac
      421 ggtaatgcca gataaataca gacacaatgg taaataatat agttgcaaat aatgctgcca
      481 taatcagcgc gattgcgaca cctgttttcg ataccagcgg caccagcaaa gcgcacagtc
      541 catagccaag tgtgtgactg gtagcgagcc agcccgcgcc tgcgccatca gccagcttat
      601 cattcagtag aagttgataa gcgggtgttg ccagagctgc tccaaatgac agcactgcac
      661 agcccgggta aaacaaccat agctgttcgg agagcatgat agccagacca ccactcatca
      721 acactccggc actcaggagc agggccaccg gagtcaggcg ctgcggacgg actaccccga
      781 actgcgcgat aagcgcagct actgcggaca gacccaacag ccaggccacc tgttggctaa
      841 tggcggtggt atccgtgacg aactggcgag taagggcagg cgaaagtcca agctgcatca
      901 tgctcaccgc cgcagccagt aatattgcgc aaagcagata aggcagacaa tcccgcttca
      961 gactgacgct cttgcactcc ggtgtgggct gtggtggtgt gccgggcaac cgcagcagca
     1021 tcagcagcgc cagcactggt gctgccatca gtagccccag tggcgccaga ggatgaatgg
     1081 ccagcattgc tgccgcgcac agcggcccga atagcctgcc gcaactcagg ccggagctga
     1141 tggttgccag agcggccatg cgattccctt ctccagctct ctgcaatgcc cagacctgac
     1201 aggctggcac cattgccgac acggtcagcc cgtaggcgat ccgggcgatg attaatattc
     1261 ccagtcccac cgctgttgtt atggcgctgg tagccatcag cacgcttccc agccctagca
     1321 gggtaaagct tatgccatat cctcccagag cccacaacac cacaggttta ctgccagtac
     1381 gggaaatttg ctttccccac cacggggacg atggcagaaa cagcattgag ccaagcatca
     1441 gcagggcagc ccatacagac agactcagat ttgtctgtat gaccagaaca gggagcacaa
     1501 ccagcagacc gttctgtccg ataccgagaa gcccggcact gaacgcaagt ggccagcagg
     1561 acagtgtttt ttggggcgta tcttcgatcc ctggtgacat tataatgctt caactccatg
     1621 tattaattgt gtttatttgt aaaattaatt tatctgacaa taacatttat tgttgataat
     1681 gagaatcatt atcgacataa ttgttattat tttactgtgt gggagctgtt tgactatgac
     1741 cctgccctct gaaaaaccag ccacagatgt ggctgcgcag tgcttcctga atgcactgat
     1801 tcgtgaaacc acagactgga aactgacaga atacccgcca gacgaattgc tcatcccgct
     1861 ggatgagcag aaatcgctcc atttcagagt ggcttatttc tccccaaccc agcatcaccg
     1921 ctttgcattt cctgcacgtc tggtcacggc atcaggcagt tatcctgtcg actttaccac
     1981 tctctcccgg cttattattg ataagctacg ccatcaactg tttctgcccg ttcccctctg
     2041 cgaaactttc caccagcgcg tgctggaaag ccatgtccat acgcaacagg caattgatgc
     2101 ccgtcatgac tgggccgctc tgcgtgaaaa agcgttgaat tttggcgagg ctgagcaggc
     2161 gctgctgaca ggacacgctt tccaccctgc gcctaagtct catgaaccgt ttaaccggcg
     2221 ggaggctgaa cgctacctgc ctgatatggc accccacttc ccactgcggt ggttttcggt
     2281 ggataaaacg caaatcgccg gtgaaagttt gcatctcaac cttcaacagc ggctgacgcg
     2341 atttgccgca gagaatgcgc ctcagttact caacgaatta agtgacaacc aatggctgtt
     2401 cccgttgcac ccgtggcagg gggaatatct tttgcagcag gggtggtgcc aggcacttgt
     2461 tgctaaaggg ctgattaaag acttaggtga ggccggcacg tcgtggctgc cgaccacctc
     2521 ttcccgttcc ctctactgtg ccaccagccg cgatatgatc aagttctccc tgagcgtacg
     2581 gctgaccaac tccatccgta ctctgtccgt gaaagaagtg aagcgtggga tgcgcctggc
     2641 acgcctggct caaaccgacg gctggcagat gctacaggtc cgcttcccga ctttccgggt
     2701 aatgcaggag gatggctggg ccgggctgct cgatcttaac ggcaacatca tgcaggaaag
     2761 tctgtttgcc ctgcgtgaaa atctgctggt ggatcagccg aaaagccaga ctaacgtact
     2821 ggtctccctg actcaggccg cacctgatgg cggtgattcg ctgctggttt cggcggtaaa
     2881 acgcctgagc gatcgcctcg gtatcactgt gcaacaggcc gcccatgcat gggtcgatgc
     2941 atactgtcag caagtgctaa agccgctgtt tacggctgaa gcggattacg gcctggtgct
     3001 gctggcgcat cagcaaaata ttcttgtcca gatgcttggg gatctgccgg tcggatttat
     3061 ttaccgtgac tgtcagggca gcgcttttat gcctcacgcg acagactggc tcgattctat
     3121 tggcgaggcg caggcggaaa atattttcac ccatgagcag ttgctgcgct attttcctta
     3181 ttacctgctg gttaactcca cttttgctgt gaccgctgcg ctgggggctg ccgggctgga
     3241 cagcgaatcg aatctgatgg ctcgtgtacg agcatcgctg gctgaagtgc gtgatcaggt
     3301 gactcataaa acctgtctca actacgtgct ggaaagtccg tactggaacg taaaaggtaa
     3361 ctttttctgt tatctgaacg atcataacga gaacaccatt gttgaccctt cggtgatcta
     3421 tttcgatttc gctaacccgc tgcaggctca ggaggtctga atgtctgagg caaacattat
     3481 tcacagcaga tatggactgc gctgtgaaaa actcgacaag cccctgaatc ttggctgggg
     3541 actggacaat agcgcggtgt tgcactgtcc cggggagctg ccgacagggt ggctgtgcga
     3601 tgcgctggat cagatattta tcgccgcacc acaactgtca gcagtggctc tcccctgggc
     3661 cgaatggcgt gaggagccac aggcgctgac gctttttggg caggtaaaaa gcgacattat
     3721 ccatcgtact gccttctggc agttaccgtt atggttgagt tcaccggcaa accgggcctc
     3781 cggtgaaatg gtttttgatg cagagcgtga gatttatttc ccgcagcgcc caccccgtcc
     3841 acagggtgag gtttaccgcc gttacgatcc acgtattcgc aggatgctga gtttccgcat
     3901 tgccgatccc gtttctgatg cagaacgttt cacccgctgg atgaatgatc cgcgcgttga
     3961 gtatttctgg gagcaaagtg gctcactgga ggtacagacc gcctatctgg agcgccagct
     4021 caccggtaaa catgcattcc cgctgattgg gtgcttcgac gatcggccat tcagctattt
     4081 cgaaatctac tgggcggcgg aagaccgcat tggtcgtcac tattcgtggc agccctttga
     4141 ccgtggcctg catctgctgg ttggtgaaca gcaatggcgc ggggcccact atgtgcaaag
     4201 ctggctgcgc gggctgacac attacctgct gctggatgag ccccgtacgc agcgcacagt
     4261 actggagcca cgcaccgata accagcgcct gttccgccat cttgagcctg cgggataccg
     4321 gacaattaaa gagttcgact tcccacataa gcgctcgcgc atggtgatgg cggatcgtca
     4381 tcacttcttc acggaggtcg gtctgtaatg aatcacaagg actgggattt tgttaaccgt
     4441 cggctggtgg caaaaatgtt gtctgagatg gagtatgagc aggttttcca cgcggaatct
     4501 cagggcgatg accactactg cattaacctg ccgggagcac aatggcgctt catcgctgaa
     4561 cgtggtatct ggggctggct gtggattgat gcccaaacgt tgcgctgcac ggacgaacca
     4621 gtactggcgc agacgctgct gatgcagcta aagccggtgc tgtcaatgag tgatgccact
     4681 gttgcagagc atatgcagga tttgtatgcc acgctactgg gcgacctgca actgctgaag
     4741 gcccgtcgcg gactgagtgc cagtgacctg attaatcttg atgccgatcg tctgcaatgc
     4801 ctgctgagcg gtcatcctaa attcgttttt aataaaggtc gccgtggctg gggcaaagag
     4861 gcgctggaac gatatgcgcc agagtatacc aacaccttca gactgcactg gctggcggta
     4921 aaacgtgaac atatgatctg gcgctgcgat aacgatctgg atattcagca gttgttgact
     4981 gccgcaatgg atccgcagga gtttacacgc ttcagtcagg tctggcagga aaacgggctg
     5041 gatcacaact ggctaccgct gccggtacat ccgtggcagt ggcagcaaaa aatcgccaca
     5101 gacttcatcg ctgattttgt cgaaggcagg atggtgtctc tcggcgagtt tggcgaccag
     5161 tggctcgccc agcagtcgct gcgtaccctg accaacgcca gccgccgggg agggctggat
     5221 atcaagctgc cgctgaccat ctacaacacc tcatgctacc gggggatccc tggcagatat
     5281 atcgctgccg gaccactggc ttcacgctgg ctgcaacagg tttttgcgac cgacgccacc
     5341 ctggtgcaaa gcggcgcagt gatccttggt gaaccggcgg caggctatgt gtcccatgaa
     5401 ggctatgccg cgcttgcccg ggctccctat cgctaccagg aaatgcttgg tgttatctgg
     5461 cgggagaatc cgtgccgctg gctgaaaccg gatgaaagtc ctgttctgat ggcaacactg
     5521 atggagtgcg acgaaaacaa tcagccgctg gcaggcgcat atatcgaccg ctccggactg
     5581 gacgctgaaa cctggcttac gcaattgttc cgggtggttg tggttccgct gtatcacctg
     5641 ctttgccgct acggtgtcgc gcttattgca cacggacaaa atataacgct cgccatgaaa
     5701 gagggggtac cacagcgtgt tctgctgaaa gacttccagg gcgatatgcg gctggtgaaa
     5761 gaagcgtttc ccgaaatgga ctctttgcct caggaggtgc gtgatgccac atcccgtctg
     5821 agtgcggact acttaatcca tgatttgcag acgggtcact tcgtgacagt actgcgtttt
     5881 atttcgccac tgatggttcg tcttggcgta cctgaaaggc gattttatca actgctggca
     5941 gcagtgttga gtgattacat gaacaaacat ccacaaatgg cagagcgttt tgcgcttttc
     6001 tcactcttca ggccacaaat cattcgcgtg gtgctgaacc cggtaaaact gacctggccg
     6061 gacctggatg gcggcagtcg catgctgccg aattaccttg agaatctgca aaatccgctg
     6121 tggctggtaa ctcaggaata tgaatcatga aaaaaagtgt cgattttatt ggtgtcggga
     6181 cagggccatt taatctcagc attgctgcat tgtcacatca gatcgaagaa ctgaactgtc
     6241 tcttctttga cgaacatcct catttttcct ggcatccggg tatgctggta ccggattgtc
     6301 atatgcagac cgtctttctg aaagatctgg tcagtgcagt tgcacctaca aatccctaca
     6361 gctttgttaa ctatctggtg aagcacaaaa agttctatcg cttccttaca agcaggctac
     6421 gtacagtatc ccgtgaagag ttttctgact atctccgctg ggctgctgaa gatatgaata
     6481 acctgtattt cagtcatacc gttgaaaata ttgatttcga caaaaaaagt cgattgtttc
     6541 tggttcagac cagtcgggga gaatattttg cccgcaatat ctgccttggt acaggaaaac
     6601 aaccttattt accaccctgt gtgaagcatg tgacacaatc ctgtttccat gccagtgaaa
     6661 tgaatcttcg tcggccggac ctgagtggaa aacggataac cgtggttggt ggaggacaga
     6721 gtggtgcaga cctgttcctt aatgcattac gcggggaatg gggagaagcg gcggaaataa
     6781 actgggtctc acggcgtaat aattttaacg cactggatga ggctgctttt gctgatgagt
     6841 attttacacc tgaatatatt tcaggcttct ccggactgaa ggaagatatt cgccatcagt
     6901 tactggatga gcagaaaatg acatcggatg gcatcactgc cgattcttta ctgaccattt
     6961 atcgtgagtt gtaccaccgt tttgaagttc tgagaaaacc aagaaatatc cgtctgctac
     7021 ccagtcgctc ggtaataact ctggaaagta gtggtccggg ctggaggtta ttgatggaac
     7081 atcatctgga tcagggcaga gagagcctgg aaagtgatgt ggtgattttc gccacaggtt
     7141 accgttctgc attgccacaa atacttccct cactgatgcc cttgatcacc atgcacgata
     7201 agaacacctt taaagtgcgt gatgacttca ctctggaatg gagtggcccg aaagaaaata
     7261 acatctttgc ggtcaacgcc agcatgcaaa ctcatggcat cgccgaaccc cagctcagcc
     7321 tgatggcctg gcgatctgca cgtattctta atcgcgtact ggggcgtgat ttattcgatc
     7381 tcagtatgcc gcccgcactg attcagtggc gcagcggcag ccggaaaaaa ccgcagccgg
     7441 aggctgcttc tttaactcac tatacagcaa atattcagga ataacgatga tgcgcaaaaa
     7501 gtatatgccc cgggctcttg gtccgctgct tcttgtcgtg ctgtcaccag ctgtcgccca
     7561 gcaaaacgat gataatgaga tcatagtgtc tgccagccgc agcaatcgaa ctgtagcgga
     7621 gatggcgcaa accacttgga ttatcgaaaa tgccgaactg gagcagcaga ttcagggcgg
     7681 taaagagctg aaagacgcac tggctcagtt aatccccggc cttgatgtca gcagccagag
     7741 tcgaaccaac tacggtatga acatgcgtgg ccgcccgctg gttgtcctga ttgacggtgt
     7801 gcgcctcaac tcttcacgtt ccgacagccg acaactggac tctgtcgatc cttttaatat
     7861 cgaccatatt gaagtgatct ccggcgcgac ggccctgtac ggtggcggga gtaccggagg
     7921 gttgatcaac atcgtgacca aaaaaggtca gccggaaacc atgatggagt ttgaggctgg
     7981 cacaaaaagt ggctttaaca gcagtaaaga tcacgatgag cgcattgccg gtgctgtctc
     8041 cggcggaaat gaccatatct ccggacgtct ttccgtggca tatcagaaat ttggcggctg
     8101 gtttgacggt aacggcgatg ccaccctgct tgataacacc cagaccggcc tgcagcactc
     8161 caatcggctg gacatcatgg gaaccggtac gctgaacatc gatgaatccc ggcagcttca
     8221 actgataacg cagtactata aaagtcaggg agacgacaat tacgggctta atctcgggaa
     8281 aggcttttcc gccatcagcg ggagcagcac accatacgtc agtaaggggc tgaattctga
     8341 ccgcattccc ggcactgagc ggcatttgat cagcctgcag tactctgaca gtgatttcct
     8401 gggacaggaa ctggtcggtc aggtttacta ccgcgatgag tcgttgcggt actacccgtt
     8461 cccgacggta aatgcgaata aacaggcgac ggctttctcc tcgtcacagc aggataccga
     8521 ccagtacggc atgaaactga ctctgaacag ccaactgatg gacggctggc aaatcacctg
     8581 ggggctggat gctgagcatg agcgctttac ctccaaccag atgttcttcg atctggctca
     8641 ggcaagtgct tccggagggc tgaacaacca taagatttac accaccgggc gctatccgtc
     8701 atatgacatc accaatctgg cggccttcct gcaatccagc tatgacatta atgatatttt
     8761 taccgttagc ggtggcgtac gctatcagta tactgagaac agggtagatg atttcatcga
     8821 ctacacgcag caacagaaga ttgctgccgg gaaggcgata tctgccgacg ccattcctgg
     8881 tggttcggta gattacgata actttctgtt caatgctggt ctgctgatgc acatcaccga
     8941 acgtcagcag gcatggttca atttttccca gggggtggca ttgccggatc cggggaaata
     9001 ttatggtcgc ggcatctatg gtgcagcagt gaacggccat ctttccctga caaagagcgt
     9061 gaacgtcagc gacagtaagc tggaaggcgt gaaagtcgat tcttatgaac tgggctggcg
     9121 ctttaccggt gacaacctgc ggactcaaat cgcggcatat tactcgcttt ccaataagag
     9181 cgtggaaagg aataaagatc tgaccatcag tgtgaaggac gacaggcgcc gtatttacgg
     9241 cgtggaaggt gcggtggact acctgatccc ggatactgac tggagtaccg gtgtgaactt
     9301 caatgtgctg aaaaccgagt cgaaagtgaa cggtcaatgg caaaaatatg acgtgaagga
     9361 atcaagtcca tcgaaagcga cagcttacat taactgggcg ccggaaccgt ggagtctgcg
     9421 tgtacagagc accacttctt tcgacgtaag cgatgcagag ggtaacgata ttaatggtta
     9481 cactaccgtc gattttatca gtagttggca gcttccggtg ggaacactca gcttcagcgt
     9541 tgagaacctc ttcgaccgtg actataccac tgtctgggga cagcgtgcac ctctgtacta
     9601 cagcccgggt tacggccctg cttcactgta cgactacaaa ggccggggcc gaacctttgg
     9661 tctgaactac tcagtgctgt tctgacgggg atgcttttgc aacaagggta tgctgtaaac
     9721 aacatggtcc ttggtggcca tgt
//
LOCUS       DAJTSL010000155         9581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTSL010000155 DAJTSL010000000
VERSION     DAJTSL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.155"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            49..732
                     /locus_tag="OO433_003374"
     CDS             49..732
                     /locus_tag="OO433_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6051730.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            776..5500
                     /gene="ehaG"
                     /locus_tag="OO433_003375"
     CDS             776..5500
                     /gene="ehaG"
                     /locus_tag="OO433_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HCR6051731.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENA
                     TAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIA
                     LGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVG
                     NSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTY
                     NLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSK
                     DAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADAL
                     LWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIAN
                     NTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTT
                     GSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTA
                     AHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPT
                     YKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADG
                     EISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALN
                     AIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYT
                     TNKYIADALDGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGA
                     GAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDA
                     TYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVD
                     TGIALGSSSVSSQVIAKGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINV
                     ADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGD
                     KGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQ
                     NSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTN
                     LDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALG
                     TGSVAEEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSI
                     DYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLS
                     KTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVY
                     KLQGSTNSQGEYSAALGAGIQW"
     gene            5868..7523
                     /gene="lldP"
                     /locus_tag="OO433_003376"
     CDS             5868..7523
                     /gene="lldP"
                     /locus_tag="OO433_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6051732.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7523..8299
                     /gene="lldR"
                     /locus_tag="OO433_003377"
     CDS             7523..8299
                     /gene="lldR"
                     /locus_tag="OO433_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6051733.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8296..9486
                     /gene="lldD"
                     /locus_tag="OO433_003378"
     CDS             8296..9486
                     /gene="lldD"
                     /locus_tag="OO433_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6051734.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADTHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         2436 a         2411 c         2474 g         2260 t
ORIGIN      
        1 caactctacc ggcattgatc gctggtcgga ggaaataagg atttttacat gaacttgaag
       61 aagatttttt tcagtgctgt cactgtttca gttctctgcg cattaacggg atgtgactac
      121 attgaagaag gcaagccaga aagtagttta ttgaaacaac aagaagaaca taacaataaa
      181 attgatctac ttgaaaaaca acaagcacag ctaaagagtc aactggaaac tattcaaaaa
      241 caacaaactg gcataataag cagtacaaag actttaactc acgtaattaa atccgttaaa
      301 gatcaacaaa acacttttat ttttaccgag tttaacccag caaaaacgaa gtatttcatc
      361 ctgaacaatg gttcggtggc tttagcgggt cgagtgttat ccattgacgc cacagagaat
      421 ggcagtgtta ttcatatttc actggtcaac ttattaagta cacctatctc aaatattggt
      481 tttaatgcga catggggtgg tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa
      541 caattacttt tcaacacatc aatgaaatcc acattgaaat tattaccagg tcaatggcaa
      601 gacattaatt tgaccctgaa gggtgtctcg cccaataacc tgggatatct gaagttagcc
      661 atcaacatgg aaaatattca gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga
      721 agcaaaaaat aaatgcagtt agtacactta taacaaggaa tagcgagcat cattaatgaa
      781 caaaatattt aaagttatct ggaaccctgc gacagggaat tatactgtta ccagcgaaac
      841 ggcaaaaagc cgtggcaaga aatctgggcg cagtaagctg ttaatttctg cgctggttgc
      901 gggtggaatg ttgtcgtcgt ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg
      961 tgttgattac ggtagtggat cagctggcga cggctgggtt gctataggca aaggggcgaa
     1021 agcaaatact tttatgaaca ccagtggttc cagtactgct gtgggttatg acgctatagc
     1081 tgaaggccaa tatagctctg ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat
     1141 ggcctttggg gttagtgcaa tatcagaagg cgatagaagt atagcactgg gtgcctcttc
     1201 gtattcattg ggccaatact caatggccct cggccgttat tcaaaagcat tgggtaaatt
     1261 gtctattgct atgggggact cttccaaagc ggaaggagca aacgccattg ccctggggcg
     1321 tagcagtgta gctagcggta ctgacagcct cgcatttggc agacaatcac ttgccagcgc
     1381 agcgaacgct attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg
     1441 caataatgcg aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga
     1501 taaagtcaat actatcgcat taggaaatgg cagccaggct ctggcagata atgcaatcgc
     1561 cataggccag ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca
     1621 gtcgagaggc ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag
     1681 tcttgcgctt ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga
     1741 ttccattgcg gatttagaca ataccgtctc tgtcggcaat agttcattaa aacgcaagat
     1801 cgttaatgtg aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca
     1861 actttatgcc attagcgact cggtagcaaa aaggcttgga ggaggggctg cagtagatgt
     1921 tgatgacggt actgttacag caccaaccta caatttaaaa aatggtagca aaaataacgt
     1981 aggggctgcg ctcgctgtac ttgatgaaaa caccctgcaa tgggaccaaa ccaaaggcaa
     2041 atacagcgct gctcatggta ctagtagccc aactgccagc gtaatcaccg atgttgcgga
     2101 tggcacgatt tcagcctcca gtaaggatgc ggttaacggt tcccaactga aagctaccaa
     2161 tgacgatgtc gaagccaaca ccgccaatat cgctactaat accagcaaca ttgccacgaa
     2221 tacggcaaat attgccacca ataccaccaa tatcaccaac ctgacggatt ccgttggtga
     2281 ccttcaggct gatgccctgc tctggaacga aactaaaaag gcattcagtg cagctcacgg
     2341 ccaggatacc accagcaaaa tcaccaacgt taaagatgcc gacctgacgg ctgacagcac
     2401 tgatgctgtt aacggctctc agctgaaaac caccaacgat gctgtggcga cgaataccac
     2461 caatatcgcc aataacactt ccaatattgc cactaacacc accaacatct ctaatctgac
     2521 tgagacggtg actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtatt
     2581 cacggcagct catggcaccg agaccaccag caaaatcacc aacgttaaag atggcgacct
     2641 gacgactggc agcaccgatg ccgttaacgg ctctcagctg aaaaccacca acgatgccgt
     2701 ggcgacgaat accaccaata tcgccactaa caccaccaac atctctaatc tgactgagac
     2761 ggtgactaat cttggtgagg atgcgctgaa atgggataag gacaatggtg tcttcactgc
     2821 agctcatggc aacaataccg ccagcaaaat caccaatatc ctggacggca cagtcactgc
     2881 aaccagttcc gatgccatta acggtagcca gctttatgac ttaagcagca atatcgccac
     2941 ctacttcggc ggcaatgctt ctgtgaatac tgacggtgtg tttaccggtc caacctacaa
     3001 aatcggtgaa acaaattatt ataacgtcgg cgatgcactg gctgcgatta actcctcatt
     3061 tagcacgtct ctcggcgatg ctctgctttg ggatgccacc gcaggtaaat tcagtgccaa
     3121 acacggtact aatggtgacg caagcgtgat cactgatgtc gcagatggtg aaatttcaga
     3181 ctccagttct gacgcagtaa acggctcaca actccacggc gtgagcagtt atgttgttga
     3241 tgcgctgggg ggtggtgccg aagtcaatgc agacggcacc atcactgcgc cgacgtacac
     3301 cattgctaat gctgattacg ataatgtcgg tgatgccctg aatgctatcg ataccactct
     3361 tgacgacgct ctgctctggg atgcggacgc cggtgaaaat ggtgcattta gcgccgctca
     3421 cggaaaagat aaaactgcca gtgtaatcac taacgtcgct aacggtgcaa tctctgctgc
     3481 cagcagcgac gcgattaacg gctcacaact ctataccacc aataagtaca tcgctgatgc
     3541 gctggatggt gacgcagaag tcaacgctga cggcaccatc accgcaccga cttacaccat
     3601 tgcgaacgcc gagtacaaca acgtcggtga cgctctggat gcgcttgatg ataacgccct
     3661 gctgtgggat gagactgcca atggcggtgc tggagcctac aatgccagcc atgacggtaa
     3721 agccagcatc atcactaatg tcgctaatgg cagtattagt gaggacagta ccgatgcagt
     3781 gaacggttct cagttgaatg cgacgaatat gatgattgag cagaacaccc aaattatcaa
     3841 tcagctcgct ggtaacaccg acgcaaccta tatccaagaa aacggtgcgg gtattaacta
     3901 tgtgcgtact aacgacgacg gcttagcgtt caacgacgcc agcgcacagg gtgttggcgc
     3961 tacagctata ggttataact ctgtcgccaa aggcgatagc agcgtagcta ttggtcaggg
     4021 cagctacagc gacgttgata cgggtatcgc cctaggtagc agctctgttt ccagccaagt
     4081 gattgccaaa ggctcccgtg acaccagcat aacggaaaat ggcgttgtta ttggttacga
     4141 caccacggat ggcgaactgc tcggtgcatt gtctatcggt gatgacggta aatatcgtca
     4201 aatcatcaac gtagccgatg gttccgaagc ccatgacgcc gttacggttc gtcaattgca
     4261 gaatgcgatt ggtgcggtcg caaccacgcc gactaaatac ttccacgcta attcaacgga
     4321 agaagattca ctggcagtgg gaactgactc gctggcaatg ggtgcgaaaa ccatcgtgaa
     4381 tggcgataaa ggtattggta tcggttatgg tgcctacgtg gacgcgaatg cacttaacgg
     4441 cattgccatt ggtagcaatg cgcaagtcat tcatgtcaac agtattgcga taggtaatgg
     4501 ttctacgacc actcgtggcg ctcaaaccaa ttataccgcc tacaacatgg acgcaccgca
     4561 gaactctgtc ggtgaattct cagtcggtag tgcggatggt caacgtcaga tcacaaacgt
     4621 cgcagctggt tcagcggata ccgatgcggt taacgtgggt cagttgaaag tcactgatga
     4681 gcgcgtagcg caaaataccc agagcattac taacctgaac aatcaggtca ctaatctgga
     4741 tactcgcgtt actaatatcg aaaacggtat tggcgacatt gtcaccaccg gtagcaccaa
     4801 gtacttcaag accaacaccg atggcgtaga tgccaacgcc cagggtaaag atagcgttgc
     4861 tattggttct ggttccattg ctgccgctga caacagcgtc gcactgggta ccggttccgt
     4921 tgcagaggaa gaaaatacaa tctctgtagg ttcttctact aaccaacgcc gtatcaccaa
     4981 cgtagctgcc ggtgttaatg ccaccgatgc ggttaacgtg tcgcagctga aatcttctga
     5041 agcaggcggc gtgcgctacg acaccaaagc tgatggttct atcgactata gcaatatcac
     5101 cctcggtggt ggcaacggca gtacgactcg tatcagcaac gtctccgctg gcgtcaacaa
     5161 caacgacgcg gtgaactacg cgcagttgaa gcaaagcgtg caggaaacga agcaatacac
     5221 cgatcagcgg atggttgaga tggataacaa actgtctaaa actgaaagca agttgagcgg
     5281 tggtatcgct tctgcaatgg caatgaccgg tctgccgcag gcttatacac cgggtgccag
     5341 catggcttct attggtggcg gtacttacaa cggtgaatcg gcagttgctt taggtgtatc
     5401 gatggtgagc gccaatggtc gttgggtcta caaattacaa ggtagtacca atagccaggg
     5461 tgaatactcc gccgcactcg gtgccggtat tcagtggtaa tgatccatta agttagtgtg
     5521 actaagggca aacgccaaat tgcctgatgc gttacgcgca tcaggcaaga taaggcgctc
     5581 cgggtcaaca atctaagggc aattctctga tgaggattgc ccttttcttt accagacatc
     5641 tccccccaca agaattggcc ctaccaattc ttcgcttatc tgacctctgg ttcacaattc
     5701 cccaattaaa actcacatca atattgccaa tacacaacat ttagttaacc attcattgtc
     5761 attatcccta cacaacacaa ttggcagtgc cacttttaca caacgtgtga caaggagatg
     5821 agcaacagac tcattacacg atgtgcgtgg actccaggag acctgcaatg aatctctggc
     5881 aacaaaacta cgatcccgcc gggaatatct ggctttccag tctgatagca tcgcttccca
     5941 tcctgttttt cttctttgcg ctgattaagc tcaaactgaa aggatacgtc gccgcctcat
     6001 ggacggtagc aatcgccctt gccgtggctt tgctgttcta taaaatgccg gtcgctaacg
     6061 cgctggcctc ggtggtttat ggcttcttct atggtttgtg gcccatcgcg tggatcatta
     6121 ttgcagcagt gttcgtctat aagatctcgg tgaaaaccgg gcagtttgac atcattcgct
     6181 cgtctattct ttcgataacc cctgaccagc gtctgcaaat gctgatcgtc ggtttctgtt
     6241 tcggcgcttt ccttgaagga gccgcaggct ttggcgcacc ggtagcaatt accgccgcat
     6301 tgctggtcgg cctgggtttt aaaccgctgt acgccgccgg actgtgcctg attgttaaca
     6361 ccgcgccagt ggcatttggc gcgatgggca ttccaatcct ggttgccgga caagtaacag
     6421 gcatcgacag ttttgagatt ggccagatgg tggggcgtca gctaccgttt atgaccatta
     6481 tcgtgctgtt ctggatcatg gcgattatgg acggctggcg cggtatcaaa gagacgtggc
     6541 ctgcggtcgt ggttgcgggc ggctcgtttg ccatcgctca gtaccttagc tccaacttca
     6601 ttgggccgga gctgccggac attatctctt cgctggtatc actgctctgc ctgacgctgt
     6661 tcctcaaacg ctggcaacca gttcgcgtat tccgcttcgg tgacttgggg gcgtcacagg
     6721 ttgatatgac gctggcccac accggttaca ccgcgggtca ggtgttacgt gcctggacac
     6781 cgttcctgtt cctgaccgct accgtaacac tgtggagcat cccgccgttt aaagccctgt
     6841 tcgcatcggg tggtgcgctg tatgagtggg tgatcaacat tccggtgccg tacctcgata
     6901 aactggttgc ccgtatgcca ccagtggtca gcgaggctac agcctatgcc gccgtgttta
     6961 agtttgactg gttctctgcc accggcaccg ccattctgtt tgctgcactg ctctcgattg
     7021 tctggctgaa gatgaaaccg tctgacgcta tcagcacctt cggcagcacg ctgaaagaac
     7081 tggctctacc catctactcc atcggcatgg tgctggcgtt cgcctttatc tcgaactatt
     7141 ccggactgtc atcaacactg gcactggcac tggcgcacac cggtcatgca ttcaccttct
     7201 tctcgccgtt cctcggctgg ctgggggtat tcctgaccgg gtcggatacc tcatctaacg
     7261 ccctgttcgc cgcgctgcaa gccaccgcag cacaacaaat tggcgtctct gatctgttgc
     7321 tggttgccgc caataccacc ggtggcgtca ccggtaagat gatctccccg caatctatcg
     7381 ctatcgcctg tgcggcggta ggcctggtgg gcaaagagtc tgatttgttc cgctttactg
     7441 tcaaacacag cctgatcttc acctgtatgg tgggcgtgat caccacgctt caggcttatg
     7501 tcttaacgtg gatgattcct taatgattgt tttacccaga cgcctgtcag acgaggttgc
     7561 cgatcgtgtg cgggcgctga ttgatgaaaa aaacctggaa gcgggcatga agttgcccgc
     7621 tgagcgccaa ctggcgatgc aactcggcgt atcacgtaat tcactacgcg aggcgctggc
     7681 aaaactagtg agcgaaggcg tgctgctcag tcgacgcggc ggcgggacgt ttattcgctg
     7741 gcgtcatgac acatggtcgg agcaaaacat cgtccagccg ctaaaaacgc tgatggccga
     7801 tgatccggat tacagtttcg atattctgga agcccgctac gccattgaag ccagcaccgc
     7861 atggcatgcg gcaatgcgcg ccacacctgg cgacaaagaa aagattcagc tttgctttga
     7921 agcaacgcta agtgaagacc cggatatcgc ctcacaagcg gacgttcgtt ttcatctggc
     7981 gattgccgaa gcctcacata acatcgtgct gctgcaaacc atgcgcggtt tcttcgatgt
     8041 cctgcaatcc tcagtgaagc atagccgtca gcggatgtat ctggtgccac cggttttttc
     8101 acaactgacc gaacaacatc aggctgtcat tgacgccatt tttgctggtg atgccgacgg
     8161 ggcgcgtaaa gcaatgatgg cgcaccttag ttttgttcac accaccatga aacgattcga
     8221 tgaagatcag gctcgccacg cacggattac ccgcctgccc ggtgagcata atgagcattc
     8281 gagggagaaa aacgcatgat tatttccgca gccagcgatt atcgcgccgc agcgcaacgc
     8341 attctgccgc cgttcctgtt ccactatatg gatggtggtg catattctga atacacgctg
     8401 cgccgcaacg tggaagattt gtcagaagtg gcactgcgcc agcgtattct gaaaaacatg
     8461 tccgacttaa gcctggaaac gacgctgttt aatgagaaat tgtcgatgcc ggtagcactg
     8521 gcaccggtgg gtttgtgtgg catgtatgcg cgtcgtggcg aagttcaggc agccaaagcg
     8581 gcggacacgc atggcattcc gtttactctc tcgacggttt ccgtttgccc gattgaagaa
     8641 gtcgcgccag ccatcaagcg cccaatgtgg ttccagcttt atgtactgcg cgatcgcggc
     8701 tttatgcgta acgcgctgga gcgagcaaaa gcagcgggtt gttcgacgct ggttttcacc
     8761 gtggatatgc cgacaccggg cgcacgctac cgtgatgcgc attcaggtat gagcggccca
     8821 aatgcggcaa tgcgccgcta cttgcaggcg gtgacgcatc cgcaatgggc gtgggatgtg
     8881 ggcctgaacg gtcgtccaca tgatttaggt aatatctcag cttatctcgg caaaccaacc
     8941 ggactggaag attacatcgg ctggctgggg aataacttcg atccgtccat ctcatggaaa
     9001 gaccttgagt ggatccgcga tttctgggat ggcccgatgg tgatcaaagg gatcctcgat
     9061 ccggaagatg cgcgcgatgc agtacgtttt ggtgctgatg gaattgtggt ttctaaccac
     9121 ggtggccgcc agctggacgg tgtactctct tccgctcgtg cgttgcccgc tattgcagat
     9181 gcggtgaaag gtgatatcgc cattctggcg gatagcggaa ttcgtaacgg gcttgatgtc
     9241 gtgcgtatga ttgcgctcgg tgccgacacc gtactgctgg gtcgtgcttt cctgtatgcg
     9301 ctggcaacag cgggccaggc gggtgtagct aacctgctaa atctgatcga aaaagagatg
     9361 aaagtggcga tgacgctgac tggcgcgaaa tcgatcagtg aaattacgca agattcgctg
     9421 gtgcaagggc tgggtaaaga gttgcctgcg gcactggctc ccatggcgaa agggaatgcg
     9481 gcatagtcgt ttgcgccccc gttactttat tcgtacccct tataatgggg tgttagccag
     9541 ccagacccgg catgattact gcccccagtc gtccatgatc c
//
LOCUS       DAJTSL010000156         9554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTSL010000156 DAJTSL010000000
VERSION     DAJTSL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.156"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(187..528)
                     /locus_tag="OO433_003379"
     CDS             complement(187..528)
                     /locus_tag="OO433_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6051735.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVEGLKL
                     IESGDWLDRDK"
     gene            complement(531..4301)
                     /gene="tamB"
                     /locus_tag="OO433_003380"
     CDS             complement(531..4301)
                     /gene="tamB"
                     /locus_tag="OO433_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6051736.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAQEEVVEPKIENPQPDEKPLGETLKDLFSRPVL
                     PEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDSNQG
                     IVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGPVDM
                     DLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMRTAV
                     KGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTLNGI
                     NTAKEFPEWPSKLNGLIKTSGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGNSYM
                     QWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKVRGT
                     VEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNINLV
                     TLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPWSLT
                     RDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDFAMLKPFM
                     PETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTLNLT
                     AELRNNRAELGWTIRLTNNGQFDGQVLVTDPQGRRNLGGNVNIRNFNLAMINPIFTRG
                     EKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMRSTL
                     AGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFEATP
                     NLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNLIVH
                     VGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGELLF
                     SGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSYLLR
                     GQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQVVVS
                     GYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(4298..6031)
                     /gene="tamA"
                     /locus_tag="OO433_003381"
     CDS             complement(4298..6031)
                     /gene="tamA"
                     /locus_tag="OO433_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6051737.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSCGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6237..6875
                     /gene="msrA"
                     /locus_tag="OO433_003382"
     CDS             6237..6875
                     /gene="msrA"
                     /locus_tag="OO433_003382"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6051738.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNEPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            7198..8541
                     /locus_tag="OO433_003383"
     CDS             7198..8541
                     /locus_tag="OO433_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6051739.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(8603..8809)
                     /locus_tag="OO433_003384"
     CDS             complement(8603..8809)
                     /locus_tag="OO433_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6051740.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            9134..>9554
                     /locus_tag="OO433_003385"
     CDS             9134..>9554
                     /locus_tag="OO433_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6051741.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGA"
BASE COUNT         2194 a         2635 c         2342 g         2383 t
ORIGIN      
        1 actcaccgtt caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt
       61 tttttatgcg attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt
      121 tacaaaaaaa aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt
      181 ttattattac ttatccctgt ctaaccagtc gccgctttca attagcttta atccctcgac
      241 gggtcgttga taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc
      301 gtattcaccg cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg
      361 ataaacttca ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct
      421 atacaactgg tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg
      481 actgttgcct tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg
      541 aactgataga gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta
      601 ggcatcaggc gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat
      661 ttcacttgca ggcctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact
      721 ccctgggtgt cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc
      781 tggccacttt gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc
      841 tgatcgctct ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc
      901 gccgggtcag agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg
      961 gcgattacgt cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt
     1021 tgatctggcg gaccagagaa cagtagctca cccttacgca caatcagatc ctgaccatag
     1081 gcatggaagc gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc
     1141 tgaacgacgt taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca
     1201 ttgttgccaa cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct
     1261 tccggttgca ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc
     1321 ggcagatcgt gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac
     1381 aggtttggtg tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc
     1441 acggtgatcc gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg
     1501 ctccagtcgg catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca
     1561 agcgtcgagc gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc
     1621 ataaagttgc cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt
     1681 tgcacatcac cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacga
     1741 gtaaagatgg ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga
     1801 ttacggcggc cttgcggatc ggtcaccagc acctgtccat caaactggcc gttattggtc
     1861 aggcggatgg tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt
     1921 gtctgaaacg ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc
     1981 cccgaaaggg tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc
     2041 gctttacccg tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg
     2101 gcgaagtcga agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt
     2161 tgcggcacgc acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt
     2221 tgctccttat tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccagc
     2281 gtctggaagc gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt
     2341 cctgccagat taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc
     2401 tgcttttcgc tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa
     2461 atttgctcaa cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct
     2521 tccacgcgaa cctgcgtcac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct
     2581 gccagtagtt gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg
     2641 ccaagccccg gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga
     2701 tcttttaccc ccagctcgcc tttcacttcg gcactgtttg gccccagttc cagatgaagc
     2761 ccagggatca tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg
     2821 ttctgtttga cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg
     2881 tacaggctac cgctggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct
     2941 ttggcggtgt taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag
     3001 tcgagcaacg ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga
     3061 ttgacctgct gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt
     3121 cccttcactg ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt
     3181 ttcagatcat ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg
     3241 ttcacttcca cgttgagcgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc
     3301 atatccaccg gaccggaaag attaacgcca atctccagct gttcgcgcag cgcgccgccc
     3361 acttttagct tcactttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg
     3421 atatccaccg gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg
     3481 ttggaatcga tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc
     3541 agcatggtgc gcacggtgat gtccgtgtcg cccgtcacgc gtaactgctc gcctttaaac
     3601 tcttcaatgt tcaggttaag cggcaaatgc aagtcggtca tttccggcaa taccgggcga
     3661 gaaaagagat ctttcagcgt ttcgccgagc ggcttttcat ccggctgcgg attttcaatt
     3721 ttcggttcga ccacttcttc ctgcgccact ttcggcagag caatcagcag gcctttcagc
     3781 gacgtcggtt tcagggtcag ggttttctcc tgccagttca ggccggaggt gaagtccatc
     3841 accgacaccg tggtgtcatc aatcttgatg ttgacgttgt ccagcgccac ccgtgtcagg
     3901 gtgatgggat acggcgtgga gagatccagc ggaccgctat cttcctcttc ttcaacctgt
     3961 tcagaaggag gcattttttt actgtcgatg ttgacctgaa tgtctttcag cgccaggtca
     4021 ttaatacaaa cgctactgtt ccacaggcac tcaagaccga ccgccagatg cagattaccc
     4081 gcttttaccg ccacgcctgg ctgctcataa cgaacgtcag acaaggtgag atcgcgccag
     4141 ccgccggtga ccttgccaat atccagtcct ggcacccagc gatccgccgc tttaaataca
     4201 agatgcaggc cgctggtggt gcccaccaga aacgccaccg atcccagcaa cagtaagata
     4261 acgataacca cgccgaggct gatttttttc cataaactca taattctggc cccagaccga
     4321 tgtaaaactg taacccgtgt tcgtctttat ccgcgaccgg tacggcaaaa tcgagtttga
     4381 ttggcccgac cggcgattcc cagcgcacgc cgaccccggt accggtttta aagtcgctgc
     4441 ggcgaatatc gcttaccgct tcgccactat cgacaaacac cgcgccccac cattttccgg
     4501 tcacgttgta ctggtattcc agcgatccgg ttatcaactt cgaggcccct ttcaggtcac
     4561 cgttggcgta tttcggggcg atagatttgt atttgtagcc gcgaatactg cgatcgcccc
     4621 cggcgaaaaa acgcagatcc ggcggtactt tgtcgaaatc accggtttca atccagccca
     4681 gcgtgccgcg tgtaacgaaa cgatggcgat cgtacagtgt gcggatccag acgttctgcg
     4741 cctggaaaac ggagaaatcg acatctgagc cccaggccgt gttggagtag tcgatagagt
     4801 agcgttgcga gtcgccccag gttggcatca ggccaccaca agaacgcgtg cggctaatca
     4861 tcaccccagg ataaaacagc atcgtggtgt tggtaatttc accctgagta aagtggtcga
     4921 gactccagcg caggttaatg gcacgctgcc aaccgctgga gagatcccag tagcgagaag
     4981 ccaccagcgt agtggagtca gattcggtat cgttcaggtc agtgcgctta aaaccgccct
     5041 gcaccaaata atattgttcc agtggattct tcagcagcgg cattttatag ctgaagtcga
     5101 gggtctgttc cggcgcggaa atactggtac tggtggtcaa actgtgaccg tatgagttca
     5161 tccacggctt tttccacgtc gctttcacgc gcggtcccac gtccgtagag taaccgaccc
     5221 cggtttcgat ggtgttttct gttcgcggcg aaaccacgcc cgtcaatggt aatactttcg
     5281 tttcgcgcgc tttatcaaat tgtggagcca ccaccaccga gttaaaccag ccggtagcag
     5341 aaagtcggcg gttcagttcc gccagatctt tcgattcata ctcatcgccc tctttaaacg
     5401 gcaccagatt ttgcaggtat tcatcgcgga tttgtgatcc ttcaaaggtc acatgcccaa
     5461 agcggtaacg ttcgccactg ttataatcaa tatcccagaa ggctttatgc aggccgagcg
     5521 caatgcccag ctgcgctttg gtaaattcgc tatcgaaata acctttacgc aacgcaatac
     5581 tggttaagga ctttttgaaa ttttcataat cgccctggtt cagtaccgta ccaatagccg
     5641 ggcgagtatc gagcaatttc aaatagtctt tatcggtccg cgcgccgccg cgcaatacca
     5701 catcggtgcc gccaattaac accggcacac ctggcgtgac tttggcgatc aatacctgcc
     5761 gccctttctt tggcggtgga cggagatcaa attcaatggt cggctggtaa taacccagcg
     5821 ctttcagacc ttcacggatg gcatcatcga cgcgtgcgcg aaagcggcgg tctggcgtca
     5881 cttcatcact ttcaatcgta gaaagctgcg cacgaacgtt cttttccagc tgtcccgata
     5941 acccctcgac ctgtagacgg acgttcgcgg cgacggcaga tccgcttaag cagagtaagc
     6001 ttacacagca taactgtcgg atatagcgca cattttctcc tgaatatcct tttttcctgc
     6061 ccctggaacg ccgttaaacg gcttaacaaa aatccagtaa tatggattaa aaagcagact
     6121 aaaccccaaa tatttcttat gttttacttt agacctattc acggtggtta ttgtgtgcaa
     6181 atacgcctct tgttacaacc ttaaccccaa tgaccgattt tcgggagagc gacaccatga
     6241 gtttatttga taaaaagcat ctggtttccc ccgccgatgc cctgcctgga cgtaacaccc
     6301 cgatgcccgt agccacgctg catgcggtca acggtcactc aatgaccaat gaacctgacg
     6361 gaatggagat tgccattttt gcgatgggtt gtttctgggg tgtggagcgt ctgttctggc
     6421 agttacccgg cgtttacagc accgccgcag gctataccgg cggctatacg ccaaatccga
     6481 cttatcggga agtgtgctcc ggtgatacgg gtcatgccga agcggtacgc attgtttacg
     6541 atccttccgt catcagctat gagcagttgc tacaggtatt ttgggagaat cacgatcccg
     6601 cccagggaat gcgtcagggc aatgaccacg gcacgcagta tcgttcagcg atttatccgc
     6661 tgaccccaga acaggatgcc gcagctcgcg ccagtctgga acgttttcag gcggcgatgc
     6721 ttgccgccga tgacgatcgt cacatcacca cggaaatcgc taacgccaca ccgttttatt
     6781 atgccgaaga tgaccaccag caatatctgc ataaaaaccc atatggttac tgtggaattg
     6841 gcggaattgg cgtctgtctg ccgccggaag catagcgtta cgggtacaaa tgtagtttgt
     6901 tgataaagtg ctctttgtct atgcctgatg cggcttaaac gacttaccag gcctacaaaa
     6961 tcgtccaaat tcaatatatt gcaaggactg cgtaggcctg atcggcatag cgcatcaggc
     7021 agtattgcat ttatcagcag tctgaatttt aaccctctgg cgactttaca gtaccttacg
     7081 ctatactagc cactgaaaat tccggttcac tttcttcgaa tcggctttca atgtgtattt
     7141 cacacaaatt aatcaacttt cccttccgag gatctggcct gaaaggtcgg ataagatatg
     7201 ttaaacagta ttttagtcat actctgcttg atcgctgtaa gtgcgttctt ctcgatgtcc
     7261 gagatctcgc ttgccgcctc acgcaaaatc aaacttaaac tgctggctga tgaaggcaat
     7321 ataaatgccc aacgcgttct gaatatgcag gaaaatcccg gcatgttctt taccgtggtc
     7381 caaatcggtc tgaacgcagt ggcgattctc ggcggtatcg tcggtgatgc ggcattttct
     7441 ccagcttttc acagcctgtt ctcccgctat atgtcggcag agctctctga gcaactgagc
     7501 tttattctct ctttctcgtt agtgactggc atgtttatcc tgtttgcgga tttaaccccg
     7561 aaacgcatcg gtatgattgc gccagaagcg gtggctttgc gtatcatcaa cccgatgcgc
     7621 ttctgcctgt acgtttgcac cccgctggtg tggttcttca acggcctggc gaacatgatc
     7681 ttccgtattt tcaaactgcc aatggtacgt aaagatgaca tcacttctga tgacatctac
     7741 gcggtagtgg aagccggtgc gctggcgggc gtgttacgta aacaggaaca cgagctgatt
     7801 gaaaacgtct ttgagctgga atcccgtacc gttccgtctt caatgacacc gcgtgaaaac
     7861 gtgatttggt ttgatctcca cgaagatgag caaagcctga agaataaggt ggcggaacat
     7921 ccgcactcta agttcctcgt ctgtaatgaa gatattgacc acatcatcgg ttatgtcgat
     7981 tctaaagacc tgctgaaccg cgtgctggct aaccaaagcc tggcactgaa cagcggcgta
     8041 caaattcgca acacgctgat tgtgccggat acgttaaccc tttcagaggc gttggaaagt
     8101 tttaaaaccg caggtgaaga cttcgcggtg atcatgaacg agtacgcgct ggtggtgggg
     8161 atcatcaccc tcaacgacgt gatgaccacg ctgatgggcg atctggtcgg tcaggggctg
     8221 gaagagcaga ttgtcgcccg tgatgagaac tcatggctga ttgacggcgg caccccaatt
     8281 gacgacgtca tgcgcgtgct ggatattgac gagttcccgc agtcgggcaa ctacgaaacc
     8341 atcggcggct ttatgatgtt tatgctgcgt aagatcccga aacgcaccga ttcggtgaaa
     8401 ttcgccggct acaaatttga agtggtggat atcgataact accgcatcga ccagctgctg
     8461 gtgacccgga tcgacagcaa ggccaccgcc ctttcgccaa aactgcctga cgctaaagat
     8521 aaagaagaaa gcgtcgcgta acccccagaa acatcaacgg ctcctgaatc aggagccgtc
     8581 ttattactgc atagcacttt ggttaagcca tctctgtttg cagacgcata acctgacggt
     8641 taacttcgga catcactgac aaatgcagct tatccttcac ttttgggata agaatcttac
     8701 ccttatcaaa ttcaaaagcg ccaacgtcct tgatgtataa ccgtccacgg aacaggatct
     8761 ttacgtactt cgccacctga agtgggttgt accgttggaa aattttcatt gttatctcct
     8821 gctgagtatt acgcctgtgc ggtgccacaa tcggcccaac tattatgaga cgcaaatttt
     8881 aatgcttagt gactatagac tatccgggca atgtttccac cgcgtataac tttttttacc
     8941 taaaggttac aattattcag aattatcttt ttaccgaagc gcggtcttca gtataagcat
     9001 tcatttttca tatgatttgt gcgcttgacc gcaaactggc atcacacttg cgggaaattc
     9061 gataaatagc acatatgatt aaaactcaga tccaagtggt cggatcacct gcatatcata
     9121 agaaggaaac accatgaccc tacgcaagat tctggcactc acctgcctgc tgttgccgat
     9181 gatggcttcc gcacatcagt tcgaaaccgg tcagcgagtg ccgccgattg gcattaccga
     9241 tcggggcgag ttggtgcttg ataaagatca gtttagctac aaaacctgga acagcgcgca
     9301 gttagtggga aaagtgcgag tactgcaaca tattgctggt cgcacctctg caaaagagaa
     9361 aaacgcgacg ctgattgaag cgattaaatc agcaaagtta ccgcacgatc gttaccagac
     9421 caccaccatt gttaacaccg acgacgcaat tccgggttcc ggcatgtttg tgcgcagcag
     9481 tctggagagt aataaaaagc tttatccctg gtcgcagttt attgtcgata gcaatggcgt
     9541 cgcgcgcggt gcct
//
LOCUS       DAJTSL010000157         9394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTSL010000157 DAJTSL010000000
VERSION     DAJTSL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.157"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(313..891)
                     /gene="mocA"
                     /locus_tag="OO433_003386"
     CDS             complement(313..891)
                     /gene="mocA"
                     /locus_tag="OO433_003386"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCR6051742.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYVNQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITVQKRYTKI"
     gene            994..1764
                     /gene="yqeC"
                     /locus_tag="OO433_003387"
     CDS             994..1764
                     /gene="yqeC"
                     /locus_tag="OO433_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCR6051743.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1812..3437
                     /locus_tag="OO433_003388"
     CDS             1812..3437
                     /locus_tag="OO433_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCR6051744.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHINRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3478..4410)
                     /gene="arcC"
                     /locus_tag="OO433_003389"
                     /pseudo
     CDS             complement(3478..4410)
                     /gene="arcC"
                     /locus_tag="OO433_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(4458..5843)
                     /gene="hyuA"
                     /locus_tag="OO433_003390"
     CDS             complement(4458..5843)
                     /gene="hyuA"
                     /locus_tag="OO433_003390"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCR6051745.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5896..7107)
                     /locus_tag="OO433_003391"
     CDS             complement(5896..7107)
                     /locus_tag="OO433_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCR6051746.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(7165..8361)
                     /gene="dpaL"
                     /locus_tag="OO433_003392"
     CDS             complement(7165..8361)
                     /gene="dpaL"
                     /locus_tag="OO433_003392"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCR6051747.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8419..>9394)
                     /gene="ygeW"
                     /locus_tag="OO433_003393"
     CDS             complement(8419..>9394)
                     /gene="ygeW"
                     /locus_tag="OO433_003393"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR6051748.1"
                     /translation="RTRFSYASALNLLGLAQQDLDEGKSQIAHGETVRETANMISFCA
                     DAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQCDIDHPTQSMADLA
                     WLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFGMDVTLAHPEGYDL
                     IPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKVMEERTELLRANDH
                     EGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADISGVSCKEGEVTEG
                     VFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQERVK"
BASE COUNT         2195 a         2292 c         2461 g         2446 t
ORIGIN      
        1 cttagcttat acagatatca ttgagtgacc ggtgttgaga aatccggtgg tttaggggga
       61 tagagcaaca atcgcaccac tcttgccagg tttggcggga aatataaaaa aatgtgaggc
      121 ggctcgaaac ttaagggctt tttgtgaatg aactcacagg tgctaaatga gaattaaaat
      181 cactcctgaa aattattatt gccagcccat atatcacttt ttctcttttc tcaaaatcag
      241 tgattcctta tcattatgat aaagagtgct ggagggatta aatatttgaa cgcaatcgtt
      301 taactacgtt ttttaaattt tagtatacct tttttgcact gtaataaagt cgtccggagt
      361 atcaatatct aaaattattt ctgcattttc tacttcgacg gaataatgag ctcccataag
      421 gagtgcctga cgcatattgg tgatattggg gcgcttgatt gcctgcatca ggcatggttt
      481 tgacactaaa atcggatggc cggggatgcc attatggagt ggcagtattg cgccatcatt
      541 tcgtaacgac cagatttttc tgaaaatatc gatggtgagg gttggcatgt caccgtgggt
      601 gagaaaacaa tgttctgttt gcactgcggg tactgcggcc tttactgatg ttagtaaacc
      661 ctgcgcataa tctgggttgt ggataatagt gatattgctc tggttcacat aacgttcgtg
      721 cagttcatta ccacgatagc cggtgactaa aataattcgg ctacaaaact gcaacgcatt
      781 tttgatactt gtatcaagaa ttgtcccctg ttgccagggt aacatcattt tccattgccc
      841 cattcttgat gataatccgg cagcggtaat tatacagtcg atggctgaca tagtggttcc
      901 attatttata ggggctaata acgcctgata attgcgttac ttcctgatgg attaagttgg
      961 ggataattta tcccagagag gtcataaaga ctcgtgaaaa gtatagttga cccatcggca
     1021 ttattcattg atttaggtgc gcagaaacgt cccactgtga tttctgttgt tggtgctggg
     1081 gggaaaacca gcttgctttt ttggctggca gaactgctcc aggctagcgg caggcgtgtg
     1141 ttaatcacga cgacaacaca tatgtttatg ccaacgtctc actggcccgt ggttttctgt
     1201 cgtgatcccg ccatgcttcc tcatgcgtct cttacatccc ccatttcatt ttgttttcac
     1261 agctggaaag cgaaccaggg aaaagtgcag ggatttacgc cagaagcgat tgatgcactg
     1321 gtgcaacgac cagagtgtga cgtaattctc attgaagcag atggctcgcg tgcaatgccg
     1381 ttaaaagcgc ctgatgagca cgaaccttgc atacccaaaa gcagttgctg cgtgattgct
     1441 gtgatgggag gacatatttt gggcgcgaaa gtgagcacag aaaatgtcca tcgctggtcg
     1501 cagtttgctg atattactgg gttaacacct gatgcaacct tgcaactgag cgatctcgtt
     1561 gcgctggttc gccaccctca gggggcgttt aaaaacgtac cgcaaggttg tcggcgagtc
     1621 tggttcatta accgtttttc tcaatgtgag aatgcgattg cacaaagcga gctccttcaa
     1681 ccgctgcaac aacacgacgt agaggcaatc tggctaggcg atatacaaga acatcctgca
     1741 atcgcgcgca gatttgtgaa ttagcggccc gggtattccg ggttactgaa aggatatcca
     1801 ttgaggttgg tatgaatatt ttcacagagg ctgcaaaact cgaagagcaa aattgtccgt
     1861 ttgcgatggc gcaaattgtt gatagccgag gctcgactcc ccgccattct gcacaaatgt
     1921 tagtgcgcgc cgatggttct atcgtcggta caattggtgg cggaatggtt gagcggaagg
     1981 tgattgaaga gtcacttcag gcattgcagg aacgtaagcc gcgattattc catggacgta
     2041 tggctcgtaa cggtgcggat gctgtcgggt cagattgtgg aggtgcaatg tcagtattta
     2101 tcagcgtcca tggtatgcgt ccacgtctgg tgttgattgg cgcggggcat atcaaccggg
     2161 cgatagccca gagtgccgcg ctattaggat ttgatattgc cgttgccgat atttatcgcg
     2221 aaagcctcaa tcctgaacta ttcccaccat caaccacgct tctccatgct gagtcgtttg
     2281 gtgcggcagt ggaagcactg gatattcgcc ctgataattt tgtcctgatt gccacgaata
     2341 atcaggatcg tgaagccctc gacaaactca ttgaacagcc cattgcatgg ttggggttgc
     2401 tggcaagtcg ccgcaaggtt cagcttttcc tgcgtcaatt gcgtgagaaa ggcgtggctg
     2461 aagaacatat tgcccgttta catgcgcccg ttggttacaa cataggtgcg gaaacgccgc
     2521 aggagatcgc catcagcgtg ctggcagaaa tattacaggt gaaaaataac gcgccgggtg
     2581 ggctgatgat gaaaccttct catccttccg gacaccagct ggtggtgatt cgcggtgcgg
     2641 gagatatcgc cagtggtgtg gcgctacgtc tgtatcatgc gggttttaaa gtgatcatgc
     2701 tggaagtgga aaaaccgaca gtgattcgtt gtaccgtggc gtttgcccag gccgtgttcg
     2761 atggcgaaat gacggtcgaa ggcgtcactg ctcgcctggc aaccagctct gcggaagcga
     2821 tgaaacttac cgaacgcgga ttcatccctg tgatggtaga tcccgcctgt tcattgcttg
     2881 atgaactgaa accgctttgc gtggtggacg ctattctggc gaaacagaat ttgggaacgc
     2941 gggcagatat ggcaccagta acaatcgcgc ttgggccggg ctttactgca gggaaggatt
     3001 gtcatgcggt aattgaaaca aatcgcgggc actggctcgg tcaggtgatt tactctggtt
     3061 gtgcgcagga gaataccggt gttcctggca atattatggg gcataccacc cgacgggtga
     3121 tccgtgctcc tgctgcaggc attatgcgat ccaacgtgaa attaggcgat ctggtgaaag
     3181 agggcgatgt gattgcctgg attggtgagc atgaaattaa agcaccgttg acggggatgg
     3241 tgcgtggctt gttgaacgac ggcctggcag tggttggtgg ttttaaaatt ggtgatatcg
     3301 atcctcgtgg tgaaaccgct gatttcacca gcgtttctga taaagcccgg gcgattggcg
     3361 gcggcgtact tgaggcgtta atgatgttga tgcatcaggg cgtgaaagcg acaaaagaag
     3421 tgctggaagt ggcttaaatg aaaatggccg gatggtatta cccacccggc cttaaaatta
     3481 ctgacaaatc accgttccgg ttttgccttc aatcccttct ttcgctttgc tcaatacggt
     3541 aatcagtgct tcacgacctg cgcgagagcg agcaaacgag gcggcagctt ccactttagg
     3601 cagcatagaa cctttcgcaa aatgaccttc ttcgataaag cgttcagcat cacttaatga
     3661 caggcgatcg agccactgtt cattctcttt accgaagtta atggccactt tttctaccgc
     3721 agtcagaatg atcagcatat cggcatcaat catttctgct aaacgggcgc tggcccagtc
     3781 tttatcgata accgcgctgg caccacgcag atggttacct tcacgaataa cagggatacc
     3841 accaccgcca acggtgatca ccacctggcc tgcatctacc agagctttaa cagtttcttt
     3901 ttcaatgata tcaaccggtt ttggcgaggc aacaacacgg cgatagccgc gaccagcatc
     3961 ttctttcaga gtgtaaccct gttttgtcag ttgctctgct tcttgctcgg taaagaacga
     4021 accgatcggc ttggtcgggt tgaggaatgc cggatcgtta gcgtcgactt cgacctgagt
     4081 aaccagcgtt gctacaggtt tattaatgcc ccgagaaagc agttcttctc ttaacacgtt
     4141 ttgtagatcg taaccaatat aaccctagct taaggcaaca cagacagaca tcggcagcat
     4201 tggcgagtgt gcttcagttt ttgcggcagc ttcaaaggcc tggttaatca tgcccacctg
     4261 cggcccgtta ccatgagtga cgataacttc atgtccttgg gcaattaaat caacaattgc
     4321 ctgagaagta atttttaccg ctttcatttg tccggccagg tcatcgccca gcgcattccc
     4381 gcccagggcg agaacaattt tcttactcat ttttcctcac tcatcattct tgattgtgta
     4441 tagatccctg acagtggtta gagcacggga gggacaaacg gtttgcgtcg caggaaacga
     4501 cctcgcccgg ctttgcctgt aaaggtgccg tcacagaaaa tcgtttcacc acgggataag
     4561 gttctgacaa tcgcgccctg acaggtaaaa ccctcccatg gcgagtagtc ggcgttgtcg
     4621 tggagatggc gatgctgaat ttgttggctc tgacgtgggt cgataatcac cacgtcgcca
     4681 tcggaaccgg gcgctaataa tcctttttgc ggccacaggc caaacagcct ggcgggcatt
     4741 gcgctggtta attcaacaaa gcgttccggt gttatacgtc ccgtcatcac gccactggaa
     4801 aacagtaact gcatgcggtt ttccacaccg ggtaagccat ttgggcagcg actgaaatcg
     4861 cctttagaaa tttgcaggcg ttgagccatc gagaaggtgc agtgatcggt cgccaccacg
     4921 tcaatcgcac catcgctgat gccacaccac agtttgtcct gctcgcgtac gttacgcagc
     4981 ggtgggctaa gaatgaactt catgccatct tctgtatcgt aactgcgttc gtccaacagg
     5041 agatattgtg ggcaggtttc aacccagact ggctggtgat tcgcacgggc aagacgcaga
     5101 taatccagac ctaagccgtt agacaggtgc acgatataga gcggggcgtt accggcaatt
     5161 tgtgccaggt taatcatgcg ggcgatggct tccgcttcgc attccagagg gcgactcaag
     5221 gcatgatagc gcggcgcggt taaccctgcg gcgataaatt ccgcccgctt gctggcgata
     5281 gccgcatcat tttccgggtg cacggtggtc agcgcgccgg attcatgcaa gcggcgtaat
     5341 gcctgcaaaa cctcgtcatc gttgagtttg tattgatagg ttaaatagag tttaaaactg
     5401 ctcagtcctt cctcgaccat catcgggatt tcgtcgagga ttgcgtgatt aatgtgctgg
     5461 atcacaccgt gaaagctgta atcgatgacc gctttatggg cggcataacc acgataaacc
     5521 tccagttgat ggcgtaaccg acagccgttt gggccaaatc ccatatggtc aataatggtt
     5581 gttgtaccgc cacacgcagc tgcgcgggta ccggtaaaaa aatcatcaca actgcgcgcg
     5641 atgccgacat caatattgaa atgcgtatgg acatccacgc cgcctgggaa aacgtaacag
     5701 ccagtggcat caatttcttc atacgggagc tgcggcgaaa tattgttgcc caactggcga
     5761 acaatcccgc tttcaatcag caaatcctgt ttggcttgtc catctgcgtt aacgacagtg
     5821 ccgtttttga tcaatacgcg catagcaaac tccaaaaacc ctccggcgaa ccggagggga
     5881 ataagtgaag agtaattact ctgttgccag ccagcttaac gggattgcag cgtacatcgc
     5941 ggcacaggtc accaggtgag atttccaggt tttttcgtta ggtgcatgcg cttcaggttc
     6001 tttacccggg ccaaagccga tcaccggaat gccgtgacgg cccatgatag atacgccgtt
     6061 agttgagaag gtccacttat caacaaccgg cgctttgcca aacagccctt cgtaggcatt
     6121 caccagtgct ttaacggtga agtgatcttc ttccactttc caggtcggga agtagcattc
     6181 ggttgggtaa accaggccag tccaggacgg acggtcgtag ttgtacatag aaacaaccgc
     6241 gttagctttc tgtactgcag gcagggcgcg gatttcgtcc agcgcgcctt cccaggtttc
     6301 gccccaggtc agacggcggt caatagagac ggcgcagcta tctgctacag cgcaacggct
     6361 tggggatgtg aagaagattt cagaaacggt gagggtgcct ttgccgagga attcgtcata
     6421 acccagacgt tgggagagtt cttgtaattc gccaagaatc ggacccattt tgaaaatggc
     6481 gttgtcaccg cgttctggcg cagaaccgtg gcagctaaca ccctgaacat caatacgaat
     6541 ttccatacga ccgcgctgac cacggtatat ctggcagtcg gttggttcgg tactgaccac
     6601 aaattccggg cgaatgccgg attgttcaat aatgtactgc cagcacagac cgtcgcagtc
     6661 ttcttcctgc acagtaccgg taaccagcag ggtatattca tcttccagac cgaggtcttt
     6721 aatgatttta ccggcataaa ccatagatgc catgccgcct tcctggtcgg aagtaccgcg
     6781 accaccgatt agctcatcag tttccatgcc ttcgtacgga tcgaagtccc agtttttgat
     6841 gttgccaatg ccgacggtat cgatatgagc gtccattgcc accagacgcg ggccgtggcc
     6901 gatataaccg agaacgttgc ccatcgggtc gatttcaact ttatcgaagc cgactttttc
     6961 catctcttct ttaatacgat gtactacgcg tttttcgtcg cagctttcac tgggaatagc
     7021 aaccatgtcg cgcaggaagc gagtcatgtc cgcctggtaa tcttttgctt tttcaagaat
     7081 cagtttgaat ggaatattct tagccattat ttttctccag attttttact ccccgttttg
     7141 cagggagtag ttccaatagg gtgattaagg tgctacagcg tgtttgcctt cccagacaac
     7201 ttcgcggtag tgcttcacgt cggtgtcgcc ttcagtactg ataaccagca ctacggcatc
     7261 tttgttcagc gccagttttt ccatcaggct ttgacgttgc gggtgataat gaaccgctgc
     7321 gagaacgccc aaaccgacag cgccggattc accggagatg atgcgcgggt cgttgccgta
     7381 cggattaccc agcacgcgca tacctaatgc ggcaacgctg tcctggcagg agatgaattg
     7441 ggtggcgcag ttacgtagga tttcccagcc cagcgggtta ggttcgccac aggccaggcc
     7501 cgccatgatg gtggccatat caccgccaac gttgacgatg tcgcctttga cgccggagcg
     7561 ataaatacag tcagctttgt caggttcaac aataatgctg tgcagatttt gcgggctgta
     7621 gacgtcgacc agataaccca gcacaccacc ggccattgct ccgacaccgg cttgcagcag
     7681 aacgtgcgtc ggggttacgc ccatttcacg catttgctcg acggcttcat ctgccagggt
     7741 tgcgtagcct tgcatgatcc aggttgggat tttggtgtaa ccttcccatg ccgtgtcctg
     7801 taccacttcc cagccgtgct gctgcgcgtg ttgcatggtc aggcgaacgg tatcgtcata
     7861 gttcatatcc gtgacgatgc actcggcacc gaggttcaga atggcgtcaa cgcgttcctg
     7921 agcagaacct ttcggcatgt aaatcaccgc attctgtccg agttgctgtg ctgcccacgc
     7981 cacaccgcgc ccgtggttgc cgtcggtggt ggtcgcgaaa gtcatttttt cgccgatggc
     8041 atttttcagg tgctcaaatg acagcgtttc gatatcaaga tgatattttt cacacaataa
     8101 ttgagcgatg gcgtacgcac cgccaagcat tttgaaggcg ttcagaccga atcgttttga
     8161 ttcgtctttg acgagaattt tcttcacacc aaaaaggttt gcgagatcgt ccagcgcaca
     8221 aagcggtgtt gggcgataac cagctatttt ctggtggaac tggcgcgcca gtttggcctg
     8281 gctttgcgaa aagagcggtg atgtttcgcc gttgaaaaat ttgttatcgg cgatatcaat
     8341 cttcaatgag aaaacggaca tatcctatcc ttttggcctc tggaaataga gggccgacca
     8401 gctgatcggc ccgaggggtt atttcacgcg ttcttgcgct tctttcagca gttgctcgag
     8461 cagtgcacca ggtttggcgt atttacggga caggatcatc gcggcgatga tataaggctt
     8521 ccagctggct tctttgtagg tagcgatacg gtatttttcg aatacgcctt cagtcacttc
     8581 accttctttg caggatacgc cgctgatatc agctggcagg cagtgcatgt acagggcttc
     8641 gccatcacgg gtcagttcca tcatctcttc agtacaatgc cagtctttgt gttgcgcgtt
     8701 ctgtgccaga cactgttttt ccagtgcttt taagccttcg tggtcgttcg cacgcagcaa
     8761 ttcagtacgc tcttccatca ctttgtaagg tgcccatgac ttcggataaa cgatgtctgc
     8821 gtctttgaag gcttcttcca tgctggtgac ctgacggaag ctaccaccgg aggctttagc
     8881 attgtttttc gccacttcaa ccacatccgg gatcaggtcg tagccttccg gatgggccag
     8941 ggtgacatcc ataccgaagc gagtcatcag accgatgatg ccttgtggta cagagagcgg
     9001 tttgccatag cttggagagt aggcccaggt catggcgatt tttttacctt tcaggttttc
     9061 cagtgaacca aagtgttcac gtaaccacgc gaggtcagcc attgactgag tcgggtggtc
     9121 aatatcgcat tgcaggttca ctaaagccgg acgctgtggc agtacaccct gcttgtaacc
     9181 gtcatcaagt gcagcgccaa cttcacgcat ataggcgttg cctgcgccca gatacatatc
     9241 gtcgcgaata ccaatagcgt cggcgcagaa ggagatcata ttggcggttt cacgcacggt
     9301 ttcgccgtga gcgatttgtg atttgccttc gtcgagatct tgttgtgcaa ggccgagcag
     9361 gttaagcgcg gaagcataag agaagcgggt acgg
//
LOCUS       DAJTSL010000158         9147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTSL010000158 DAJTSL010000000
VERSION     DAJTSL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.158"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..866
                     /gene="maeB"
                     /locus_tag="OO433_003394"
     CDS             <1..866
                     /gene="maeB"
                     /locus_tag="OO433_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6051749.1"
                     /translation="IGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYFQ
                     IMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSVVKNVFGYR
                     DGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFGIEPRVALL
                     SHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDRMPDSPLKG
                     SANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASVRRIVNMVA
                     LAVVEAQTQPL"
     gene            1158..1493
                     /gene="eutS"
                     /locus_tag="OO433_003395"
     CDS             1158..1493
                     /gene="eutS"
                     /locus_tag="OO433_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6051750.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            1506..1985
                     /gene="eutP"
                     /locus_tag="OO433_003396"
     CDS             1506..1985
                     /gene="eutP"
                     /locus_tag="OO433_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6051751.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            1960..2661
                     /gene="eutQ"
                     /locus_tag="OO433_003397"
     CDS             1960..2661
                     /gene="eutQ"
                     /locus_tag="OO433_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCR6051752.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGVLHVRHEGQTMIAKAGDVMFIPKGSSIEFGMTSS
                     VKFLYVAWPANWQSL"
     gene            2658..3467
                     /gene="eutT"
                     /locus_tag="OO433_003398"
     CDS             2658..3467
                     /gene="eutT"
                     /locus_tag="OO433_003398"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCR6051753.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQKPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVLEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGERNDY"
     gene            3457..4473
                     /gene="pta"
                     /locus_tag="OO433_003399"
     CDS             3457..4473
                     /gene="pta"
                     /locus_tag="OO433_003399"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6051754.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSFIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YNIAQRLGGYRAVGPLIQGLAVPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            4512..4805
                     /gene="eutM"
                     /locus_tag="OO433_003400"
     CDS             4512..4805
                     /gene="eutM"
                     /locus_tag="OO433_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCR6051755.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            4912..5199
                     /gene="eutN"
                     /locus_tag="OO433_003401"
     CDS             4912..5199
                     /gene="eutN"
                     /locus_tag="OO433_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCR6051756.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            5211..6614
                     /locus_tag="OO433_003402"
     CDS             5211..6614
                     /locus_tag="OO433_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCR6051757.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            6625..7461
                     /gene="eutJ"
                     /locus_tag="OO433_003403"
     CDS             6625..7461
                     /gene="eutJ"
                     /locus_tag="OO433_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCR6051758.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVAELLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            7451..9073
                     /gene="eutB"
                     /locus_tag="OO433_003404"
     CDS             7451..9073
                     /gene="eutB"
                     /locus_tag="OO433_003404"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HCR6051759.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAYEDDCVT
                     RLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSN
                     ADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEGLSFGVGDAV
                     IGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAPGGLIFQS
                     ICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQV
                     TMEARNYGLARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDC
                     CYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLNL
                     RPSPEFERWLESMGIMANGRLTKRAGDPSLFF"
BASE COUNT         2098 a         2382 c         2717 g         1950 t
ORIGIN      
        1 ttatcggtcg tccgaacgtg atcgaaatgc gcattcagaa actgggcttg cagatcaaag
       61 cgggcgttga ttttgagatc gtcaataacg aatccgatcc gcgctttaaa gagtactgga
      121 ccgaatactt ccagatcatg aagcgtcgcg gtgtcactca ggaacaggcg cagcgggcgc
      181 tgatcagtaa cccgacagtg atcggcgcga tcatggttca gcgtggggaa gccgatgcaa
      241 tgatctgcgg tacggtgggc gattatcatg aacattttag cgtggtgaaa aatgtctttg
      301 gttatcgcga tggcgttcac accgcaggtg caatgaacgc gctgctgctg ccgagtggta
      361 acacctttat tgccgatacc tacgtcaatg acgaaccaga cgcagaagag ctggcggaga
      421 tcaccttgat ggcggcagaa actgtccgtc gttttggtat tgagccgcgc gttgctctgt
      481 tgtcgcactc caactttggt tcttctgact gcccgtcgtc gagcaaaatg cgtcaggcgc
      541 tggaactggt cagggatcgt gcgccagatc tgatgattga tggtgaaatg cacggcgatg
      601 cagcgctggt ggaagcgatt cgcaacgacc gtatgccgga cagccctttg aaaggttctg
      661 ccaatattct ggtgatgccg aacatggaag ctgcccgcat tagttacaac ttactgcgcg
      721 tttccagctc ggaaggtgtg actgtcggtc cggtgctgat gggcgtggcg aaaccggttc
      781 acgtgttaac gccgatcgca tcggtgcgtc gtatcgtcaa catggtggcg ctggccgtgg
      841 ttgaagcgca aacccaaccg ctgtaattct tttttaactc tcacgcttac cctgaatatt
      901 cagggtaagc ggtttggctg caatatatta gtaaagctta ttactgagtt tgcgaataat
      961 aaaaaagcag tctatataat atctcgatat tattttattt atattcatgc gttgcatatg
     1021 aaagtttatg caccacagcg aatatctctc cattcttagt gatctacctc accttttaaa
     1081 cgcgcttgcc gaattttgtt atttactctg acgaaaaatt gtcacgatac acgaaagttt
     1141 ttcacaggcg gcgactcatg gataaagaac gcatcattca ggaatttgtg cccggcaaac
     1201 aggtcacact ggcgcatctc atcgcgcatc ccggtgagga actggcgaag aaaatcggcg
     1261 ttccggatgc gggcgcaatt ggcattatga cgctaactcc cggcgaaacg gcgatgatcg
     1321 ccggtgattt agcgctgaaa gccgctgatg tgcatatcgg ttttctcgac cgcttcagcg
     1381 gtgcattggt gatttatggc tcggtgggcg cagtagaaga agcgttatca caaaccgtca
     1441 gcggtttagg tcgcttatta aattatacgc tttgtgaaat gaccaaaagt taatacgagg
     1501 cattcatgaa acgaattgcg tttgtgggta gtgtcggggc aggaaaaaca acgttattta
     1561 atgcgttgca ggggaattat accctcgcca gaaaaacaca ggccgtggaa tttaatgata
     1621 agggcgatat tgatacgccg ggtgaatatt ttagccatcc ccgctggtat cacgccttaa
     1681 ttaccacgct acaggatgta gacatgctga tctatgtcca tggcgcaaat gacccggaaa
     1741 gtcggttgcc tgccgggttg ctggatattg gcgtcagcaa acggcaaatc gccgtcatca
     1801 gtaaaacgga catgccagat gctgacgtcg ccgcaacgcg aaagttattg cttgagacgg
     1861 gtttcgaaga gccgattttc gagctaaaca gccacgaccc gcaaagcgtg cagcaactgg
     1921 tggattatct ggcatctctc accaaacagg aggaagcagg tgaaaaaact catcacagcg
     1981 aatgatattc gtgaagcaca cgcgcgcggc gaacaggcaa tgtccgtggt tctgcgcgcc
     2041 agcattatta ccccggaagc ccgtgaagtg gcggatctac tgggttttac catcaccgaa
     2101 tgtgatgaat cgatcccggt aacggcgtcc gtgcccgcca gcgtgccagc cgacaaaacc
     2161 gaaagccagc gcatccgcga aaccatcatt gcccagctgc cggaaggcca gtttaccgaa
     2221 agcctggtcg cacagctgat ggaaaaagtg atgaaggaaa aacagtcgct ggagcagggc
     2281 gcaatgcagc cgagcttcaa atcggtgacc ggcaaaggcg gcatcaaagt gattgatggc
     2341 agcagcgtca agtttggtcg tttcgacggc gcagagccgc attgcgtggg cttgactgat
     2401 cttgtgaccg gagacgacgg cagcagcatg gccgccggtt ttatgcagtg ggagaacgca
     2461 ttcttcccgt ggaccctgaa ctacgacgaa atcgacatgg tgctggaagg ggtgctgcat
     2521 gttcgccacg aaggccaaac catgatcgcc aaagcaggcg acgtgatgtt tatcccgaaa
     2581 ggctccagca tcgaatttgg tatgacatcc agcgtgaaat tcctgtatgt cgcctggccg
     2641 gctaactggc aatccctatg aaagatttca tcaccgaagc atggctaaga gcgaaccata
     2701 cgctcagcga aggcgcagag atccatctcc ctgcggacag tcgcctgacg ccctctgccc
     2761 gggagttgct ggaaagccgc catctgcgca tcaagtttat tgacgagcag ggccgcctgt
     2821 ttgttgacga tgaacagcag aagccgcagc ccgttcatgg gctgaccagt agcgatgaac
     2881 atccgcaggc gtgctgcgaa ctgtgccgcc agccggtggc aaaaaagccg gatacgctga
     2941 ctcacctgtc ggcggaaaaa atggtcgcca aaagcgatcc gcgtctgggt tttcgcgcgg
     3001 ttctcgacag caccattgcg ctggcagtgt ggctgcaaat tgaactggcg gaaccgtggc
     3061 agccgtggct ggcggatatc cgttcgcgtc ttggcaacat tatgcgcgcc gatgcgctgg
     3121 gggaaccgct gggctgccag gcgattgtcg ggctttctga cgaagatctg catcggcttt
     3181 ctcatcagcc gctgcgctat ctcgaccacg atcatctggt gctggaagcc agccacgggc
     3241 gcgatgctgc gttgctcaat ctgctacgca ccaaagtgcg ggaaaccgaa accgtagcgg
     3301 cgcaggtctt tatcacccgc agctttgaag tattacgccc ggacattttg caggcgctga
     3361 accgcctttc cagcacggtc tacgtgatga tgatcctgag cgtaacgaag cagccgctaa
     3421 cggtgaagca gatccaacaa cgactggggg aacgcaatga ttattgaacg ttgtcgtgaa
     3481 ctggcgttgc gagcgcccgc cagagtggtt tttccggatg cgttagatca acgtgtgctg
     3541 aaagccgcgc aatatttaca tcaacaaggt ctggcaacgc ccattctggt cgccaatccg
     3601 tttgaacttc gtcagtttgc gctcagtcac ggcgtggcga tggacgggct acaggtgatt
     3661 gatccgcatg gcaacctcgc aatgcgggaa gaatttgctc atcgctggct ggcccgcgcg
     3721 ggcgaaaaaa cgccgccgga tgcgctggaa aaacttaccg accctctgat gttcgccgca
     3781 gcaatggtca gcgccggtaa agcggatgtc tgtatcgcag gcaacctctc ttccacggca
     3841 aatgtgctgc gtgccggatt acgcattatc ggcttgcagc caggctgtaa aacgctctct
     3901 ttcattttcc tgatgttgcc acagtacagc ggcccggcgt tgggctttgc cgattgcagc
     3961 gtggtgccac agccgacggc ggcgcagctg gcggatatcg cgcttgccag tgccgaaacc
     4021 tggcgcgcca tcaccggaga agagccgcgc gtggcgatgc tgtcgttttc cagcaatggt
     4081 agcgcccgtc acccctgtgt tgctaacgtc cagcaggcga cagaaatcgt ccgtgagcgc
     4141 gcaccaaagc tggtggtgga tggcgagtta cagtttgacg ccgccttcgt gccggaagtg
     4201 gcggcgcaaa aagcgcctgc cagcccgcta cagggcaagg ccaatgtgat ggtttttccg
     4261 tcgctggaag ccggaaatat tggttacaac atcgcacaac gactcggcgg atatcgtgcc
     4321 gtcgggccac tgatacaagg acttgccgtg ccgatgcacg atctctctcg tggttgtagc
     4381 gtgcaggaaa ttatcgagct ggcgctggtg gcagctgtgc cgcgtcagac agaagtgaac
     4441 cgcgaaagca gtttacaaac actggttgaa tgaacggtcc cgttctggac cctgataaag
     4501 aggaaagcac aatggaagca ttaggaatga tcgaaacccg gggcctggtt gcactgattg
     4561 aggcttctga cgcgatggtg aaagctgcgc gcgtcaagct ggttggtgtt aagcagattg
     4621 gcggcggcct gtgtactgcc atggttcgtg gtgatgttgc agcctgtaaa gccgcgaccg
     4681 atgcaggtgc cgcagcggca cagcgtatcg gcgagctggt atctgtacac gttatcccgc
     4741 gtccgcacgg cgacctggaa gaagtcttcc cgatcggcct gaaaggcgat agcagcaacc
     4801 tgtaatcacc ctgtgtgacg tcgattcggg cgttacgctc tctccttttt cattttacct
     4861 tctgcggagg gtagggggag cttttacctg aaatacgcca cggaggcggg tatgaaactg
     4921 gcagtcgtca ctggacaaat tgtttgtacc gtacgccatc acggactggc gcatgacaaa
     4981 ttgctgatgg tggaaatgat tgatccacaa ggtaatcctg acgggcaatg cgccgtcgcc
     5041 atcgacaata ttggcgcggg aaccggggag tgggtgttac tggtgagtgg cagttccgcc
     5101 cgccaggcgc ataaaagcga aacgtcaccg gtcgatctgt gcgtgattgg cattgtcgat
     5161 gaggtggtgt ctggcggtca ggtaattttc cacaaataag gcagaacatc atgaatcaac
     5221 aggatattga acaggtggtg aaagcggtac tgctgaaaat gcaaagcagt gacacgccgc
     5281 ccgccgccgt tcatgagatg ggcgttttcg cgtccctgga tgacgccgtt gcggcagcca
     5341 aagtcgccca gcaagggtta aaaagcgtgg caatgcgcca gttagccatt gctgccattc
     5401 gtgaggcagg cgaaaaacac gccagagatt tagcggaact tgccgtcagt gaaaccggca
     5461 tgggccgcgt tgaagataaa tttgccaaaa acgtcgctca ggcgcgcggc acgccaggcg
     5521 ttgagtgtct ctctccgcag gtgctgaccg gcgacaacgg cctgacttta attgaaaacg
     5581 cgccctgggg cgtggtggct tcggtaactc cgtccactaa cccggcggca accgtaatta
     5641 ataacgccat cagcctgatt gccgcgggca acagcgtcat ttttgccccg catccggcgg
     5701 cgaaaaaagt ctcccagcgg gcgatcacgc tgctcaacca ggcgattgtt gccgcaggcg
     5761 ggccggaaaa cttactggtt actgtggcaa atccggatat cgaaaccgcg cagcgtttgt
     5821 tcaagtttcc gggcatcggc cttctggtgg tgaccggcgg cgaagcggta gtagaagcgg
     5881 cgcgtaaaca caccaataaa cgtctgattg ccgcaggcgc tggcaacccg ccggtagtgg
     5941 tggatgaaac cgccgacctc gcccgtgccg ctcagtccat cgtcaaaggc gcttctttcg
     6001 ataacaacat catttgtgcc gacgaaaagg tactgattgt tgttgatagc gtagccgatg
     6061 aactgatgcg tctgatggaa ggccagcacg cggtgaaact gaccgcagaa caggcgcagc
     6121 agctgcaacc ggtgttgctg aaaaatatcg acgagcgcgg aaaaggcacc gtcagccgtg
     6181 actgggttgg tcgcgatgcg gcgaaaatcg cggcggcaat cggcctgaac gtgccgcaag
     6241 aaacgcgttt gctgtttgtg gaaaccactg cagaacatcc gtttgccgtg accgaactga
     6301 tgatgccggt gctgcccgtc gtgcgcgtcg ccaacgtggc ggatgccatt gcgctggcgg
     6361 tgaaactgga aggcggttgc caccacacgg cggcaatgca ctcgcgcaac atcgaaaaca
     6421 tgaaccagat ggcgaatgct attgatacca gcattttcgt taagaacgga ccgtgcattg
     6481 ccgggctggg gctgggcggg gaaggctgga ccaccatgac catcaccacg ccaaccggtg
     6541 aaggggtaac cagcgcgcgt acgtttgtcc gtctgcgtcg ctgtgtatta gtcgatgcgt
     6601 ttcgcattgt ttaaggagta acagatggcg cacgacgaac aatggctcac cccacgtctg
     6661 caaacggcgg caacgctgtg taaccagacg cccgccgcga cggaatctcc gctgtggctg
     6721 ggggtggatc tgggcacctg cgatgtggtg tcgatggttg tcgaccgcga cggtcagccg
     6781 gtggcggtat gcctcgactg ggccgatgtg gtccgcgacg gcatcgtctg ggatttcttc
     6841 ggcgctgtca ccattgttcg tcgccatctc gacacgctcg agcagcaatt cggtcgccgt
     6901 tttagccatg cggcgacttc atttcctcca ggtacggacc cgcgtatttc catcaacgtg
     6961 ctggagtctg ctggactgga agtgagccat gtgctggatg agccaaccgc agttgcggag
     7021 ctgctgcaac tggacaacgc cggtgtagtg gatatcggcg gcggcactac cggcattgcc
     7081 atcgtgaaaa agggcaaggt gacgtactcg gcggatgaag caaccggcgg acatcacatc
     7141 tctctgactc tcgccggaaa tcgccgtatt tcgctggaag aggcggagca gtacaagcgc
     7201 ggtcacggtg aagagatttg gcctacggtg aaaccggtgt acgaaaaaat ggcggacatt
     7261 gtcgcccggc atattgaagg gcagggcatt actgatttat ggctggcggg cggctcttgt
     7321 atgcaaccgg gcgtggcgga actgtttcgc aaacaattcc cggcgttaca ggtgcattta
     7381 ccgcagcaca gtttgtttat gaccccgctg gcgatcgcca gtagtgggcg agagaaagcg
     7441 gaggggctat atgcaaaatg aattgcagac cgcgctcttt caggcgttcg ataccctgaa
     7501 tctgcaacgg gtaaaaacat ttagcgttcc accggtgacg ctttgcggtc cgggctcggt
     7561 gagcagttgc ggacagcaag cgcaaacgcg tgggctgaaa catctgttcg tgatggcaga
     7621 cagctttttg catcaggcag ggatgaccgc cgggctgacg cgcagcctgg ctgttaaagg
     7681 catcgccatg acgctctggc catgtccggt gggcgaaccg tgcatcaccg acgtgtgtgc
     7741 agccgtggcg cagttgcgtg agtcaggctg tgatggggtg atcgcatttg gcggcggctc
     7801 ggtgctggat gcggcgaaag ccgtggcgtt gctggtgacg aacccggata gcacgctggc
     7861 agagatgtca gaaaccagcg ttctgcaacc gcgcttgccg ctgattgcct atgaagatga
     7921 ctgcgtgacg cggctgattc aggacgatgt taacgaaacg gcctacaacc agattaaaaa
     7981 ctggagcatc agcgaactgc gtgagtatgt gctgagcgat gaaactagcg tggacgacat
     8041 tgcctttacc cgcaaagggc tgacctcgga agtggtcgcg gcggtagcga agatttgctc
     8101 caacgcggac ctgatctacg gcgcgaagaa aatgccggta atcaaaaagg ccaataccac
     8161 catcggtatt ccgggcacct tcagcgcccg tttgcagcca aatgacactc gtgacgacgt
     8221 gcaaagtatc gccgcgcaaa tctacgaagg gctttccttc ggggtgggcg atgcggtgat
     8281 cggcgtcaac ccggtaactg acgacgtgga aaacttaagc cgcgtgctgg ataccattta
     8341 tggcgtgatc gacaaattca acatcccaac tcagggctgc gtactggcgc acgtcaccac
     8401 ccagatcgaa gcgattcgtc gcggcgcgcc tggcggactg attttccaga gtatctgtgg
     8461 cagcgaaaaa gggctgaaag agtttggcgt ggaactggcg atgctcgacg aagcgcgcgc
     8521 agtgggtgca gagttcaacc gtatcgccgg ggaaaactgc ctctacttcg aaaccggaca
     8581 aggctctgcg ctatccgctg gcgctaactt cggcgctgac caggtgacga tggaagcacg
     8641 taactatggg ctggcgcgtc attacgatcc gtttatcgtc aacaccgtgg ttggctttat
     8701 tgggccggag tatctctaca acgaccgcca gattatccgt gctggcttag aagatcactt
     8761 tatgggcaag ctgagcggca tctctatggg ctgtgactgc tgctacacca accacgctga
     8821 cgctgaccag aacctcaacg aaaacctgat gatcctgctc gccaccgcag gctgcaacta
     8881 catcatgggg atgccgctgg gtgatgacat catgctcaac taccagacca ccgcattcca
     8941 cgataccgcc actgtgcgtc agttactcaa cctgcgcccg tcaccagagt ttgaacgctg
     9001 gctggaaagc atgggcatta tggcaaacgg tcgcctgacc aaacgggcgg gcgatccgtc
     9061 actgttcttc tgatgacgcg gagataacac atcatggatc aaaaacagat tgaagaaatt
     9121 gtacgcagcg tgatggcgtc aatggga
//
LOCUS       DAJTSL010000159         9089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTSL010000159 DAJTSL010000000
VERSION     DAJTSL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.159"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            52..936
                     /locus_tag="OO433_003405"
     CDS             52..936
                     /locus_tag="OO433_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6051760.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO433_003406"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO433_003406"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR6051761.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGRQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLARVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO433_003407"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO433_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6051762.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="OO433_003408"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="OO433_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6051763.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="OO433_003409"
     CDS             5160..6443
                     /locus_tag="OO433_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR6051764.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAMNKRSVT
                     DVDSGESISIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="OO433_003410"
     CDS             6590..8065
                     /locus_tag="OO433_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6051765.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPSQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATRGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVSDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIADRIRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            8159..8245
                     /locus_tag="OO433_003411"
                     /pseudo
     CDS             8159..8245
                     /locus_tag="OO433_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            8246..>9089
                     /locus_tag="OO433_003412"
     CDS             8246..>9089
                     /locus_tag="OO433_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6051766.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGREVAK
                     FSVPL"
BASE COUNT         2394 a         2181 c         2106 g         2408 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaggtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacatcatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta ccctcgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 cagtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgcct gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccaat acccggagtc acggtcaggc
     2221 caccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agccagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccagt aagcggtaat tgaaagttta tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgc tcaacgacac
     2581 ctacgcctgc gcccaaggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg ccgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gcctggtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg aggctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcgtttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gttatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacgga acccggcgat gagaacaacc aggacatctc
     3781 cgcgctggtt gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aattcgttga
     3901 gatgtttaaa gcaccgataa aagtgctgca tcccttgtta accgccactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgagtcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tggaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct acaacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc atgaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccatatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caagcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccaa gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tagtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacatta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca ttcttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgt tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgaccagg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcaggagc tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgcgacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgagtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgccct gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggatcgt atacgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actaactgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc ggcattttat
     8281 cgccgcaagt gtcattgtgc tgacatcttc cttccttatt tttgaacttg tcgccagcga
     8341 cagagcaatg agtgcctatc tgcgctatat cgtgcagaaa gcagactcct cctttcttta
     8401 tgataagtat cagaatcaga gtattgccgc gcatgtgatg cgcgctctcg ctgctgagca
     8461 gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt ctgccaataa
     8521 tacccatggc ttaaacctga ctgctcataa atacccgggc ttacgcggca cactacaaac
     8581 cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg cttttgatca
     8641 ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga tggccgaaga
     8701 gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg agccggttaa
     8761 tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta ttggtatcga
     8821 tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca tttaccagga
     8881 tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtgtatgttg cagggcagct
     8941 aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct tttacactca
     9001 tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg attcggggcg
     9061 tgaagtggcc aaattcagtg tgccacttc
//
LOCUS       DAJTSL010000160         9030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTSL010000160 DAJTSL010000000
VERSION     DAJTSL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.160"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..482)
                     /gene="rcdA"
                     /locus_tag="OO433_003413"
     CDS             complement(<1..482)
                     /gene="rcdA"
                     /locus_tag="OO433_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6051767.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     R"
     gene            566..1774
                     /locus_tag="OO433_003414"
     CDS             566..1774
                     /locus_tag="OO433_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6051768.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            1774..2589
                     /gene="ybjI"
                     /locus_tag="OO433_003415"
     CDS             1774..2589
                     /gene="ybjI"
                     /locus_tag="OO433_003415"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6051769.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            2675..2959
                     /locus_tag="OO433_003416"
     CDS             2675..2959
                     /locus_tag="OO433_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051770.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(3000..4230)
                     /gene="mdfA"
                     /locus_tag="OO433_003417"
                     /pseudo
     CDS             complement(3000..4230)
                     /gene="mdfA"
                     /locus_tag="OO433_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            4515..5111
                     /gene="ybjG"
                     /locus_tag="OO433_003418"
     CDS             4515..5111
                     /gene="ybjG"
                     /locus_tag="OO433_003418"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6051771.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5169..5927
                     /gene="deoR"
                     /locus_tag="OO433_003419"
     CDS             5169..5927
                     /gene="deoR"
                     /locus_tag="OO433_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6051772.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPHSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(5974..7176)
                     /gene="dacC"
                     /locus_tag="OO433_003420"
     CDS             complement(5974..7176)
                     /gene="dacC"
                     /locus_tag="OO433_003420"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6051773.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKDVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            7423..8048
                     /gene="gstB"
                     /locus_tag="OO433_003421"
                     /pseudo
     CDS             7423..8048
                     /gene="gstB"
                     /locus_tag="OO433_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(8045..>9030)
                     /gene="yliI"
                     /locus_tag="OO433_003422"
     CDS             complement(8045..>9030)
                     /gene="yliI"
                     /locus_tag="OO433_003422"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCR6051774.1"
                     /translation="DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDV
                     VLAPDFAQSRRIWLSYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGN
                     HFGGRLVFDGKGYLFIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGA
                     RAEIWSYGIRNPQGMAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYS
                     GFKIPEAKGEIVAGTEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIV
                     MSVNGDKVTEDGRILTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
BASE COUNT         2167 a         2336 c         2243 g         2284 t
ORIGIN      
        1 cggtcggtga caaaatgcag cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt
       61 ccgggttcaa accattgttc gagcgtttgc tgactgcgct gcatccagtt ttgcattacc
      121 gtttttaata gcggttttcg gctagccagc gcgtagagct ggtacatcag ctccatatta
      181 tccggcgttg caacctgtga gctgtagatc atatcggtga tagcctggca tgcgccttga
      241 gcatcactaa catcgctaaa aaatgcctga tattgccggg acataatctc agtaaaactg
      301 ctgaacgcct ccagcaacaa ctcatcaatt cctgaaaaat agtaggtcat cgatcccaac
      361 ggtaccccgg caagggtagc aattttgcgg tgcgtaacag catgtattcc gtaaagtttc
      421 accgcctcca gcgtggcctg gataattttt tctcgccgtt gcggatcgtt agcgcgacgc
      481 atgtcatttc ctctgtctgc aattgtgtac aaatgtacac aaccttgcta atgttgtctt
      541 ccctcttctt attttgacgt gatctatgac cgtaaattct tcacgtaatg cattgaaacg
      601 ccgaacctgg gcgctgttta tgttcttctt tttgccaggc ctgttaatgg cgtcctgggc
      661 aacccgtacg cctgctatcc gcgatattct ctctgtctcg atcgctgaaa tgggcggtgt
      721 tctctttggt ctgtcgatcg gttcgatgag cggtattctc tgctcggcgt ggttagtgaa
      781 acgctttggg acacgtaatg tcatcctggt cacgatgtcc tgcgcattga tcgggatgat
      841 gatattaagt ctggcactct ggctgacatc gcccctgctc tttgccgttg gtctcggcgt
      901 ctttggggca agttttggtt ctgcggaagt ggcgataaac gttgaaggtg ccgccgttga
      961 gcgagaaatg aataaaacgg ttttgccgat gatgcacggt ttttatagcc tgggcacgct
     1021 ggcaggcgct ggtgtcggga tggcactgac ggcctttggc gttccggcaa cggtgcacat
     1081 tttattggcg gcgctggtag gtatcgcgcc tatttatatc gccattcagg caatccctga
     1141 cggtacgggc aaaaatgctg ccgatggcac ccagcatggc gaaaaaggca ttccttttta
     1201 tcgcgatatc cagttgctgt tgattggtgt tgtagtgctg gcgatggcct ttgccgaagg
     1261 ttctgccaac gactggttac ccttattaat ggttgacggt cacggtttta gccctacttc
     1321 cggctcgctg atttatgccg gttttaccct ggggatgacc gttggacgct tcactggcgg
     1381 ttggttcatc gaccgttaca gtcgcgttgc cgtggttcgg gccagtgcac taatgggggc
     1441 gttgggtatt gggctgatta tttttgtcga tagcgcctgg gtcgctgggg tgtctgttgt
     1501 actctgggga ctgggtgcct cgctgggctt cccgctgacc atttctgccg ccagcgatac
     1561 cggccccgat gcaccaaccc gcgtcagtgt ggtagcaacg accggttatc tggctttcct
     1621 cgtcgggccg ccgctgctgg gctatctcgg cgaacattat ggattacgta gtgcaatgct
     1681 ggttgtactg gcgctggtta ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga
     1741 taccaaaacg cagacggcga tggagaatag ttgatgagca ttaaattaat tgcggtagac
     1801 atggatggta ctttcttaag cgatcaaaaa acctataacc gtgagcggtt tatggctcag
     1861 tatcagcaaa tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa tcaatattat
     1921 cagttgatct ctttcttccc tgaaattgct aatgaaattg cctttgtggc tgaaaacggc
     1981 ggctgggtag tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgccttt
     2041 actactgtcg tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa
     2101 aatagcgcct atacgctcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat
     2161 tatcaccgtc tggaatacgt tgataacttt gacaacttag aagatatttt ctttaagttt
     2221 ggcctgaatc tttccgatga actgattcca caagtacaaa aagcattaca tgaggccatc
     2281 ggcgatatta tggtgccagt ccacaccggc aacggcagca tcgatctgat tatccccggc
     2341 gtacataaag ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa
     2401 gtggtggtct ttggcgatgg cggtaacgat attgagatgc tgcgtcaggc aggctttagt
     2461 tttgcaatgg aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg ggcaggctcc
     2521 aataaccgtg aaggcgtact ggatgtgatc gataaagtgc ttaaacacga agcgccattt
     2581 gaccaataaa aacggcagca cgcccggcaa cacaatacct gaatgaaggc tgcataattc
     2641 gcggaaatta acccgctgca ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt
     2701 gggcgcgctt ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt
     2761 gccgagtgga tacaaagtgg ttgtagttcc tgattcagtc agcgttccac aagcggtcag
     2821 cgtcgcgact gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc
     2881 gcatccttat gctcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat
     2941 tgacgaccat caccattaat tcgagaatgc ctgatcgcac aaagcagtca ggcatttttt
     3001 tacccttcgt gagaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc
     3061 cacaaaattc cgttgacaag gttgaagaga ttaaactgtc cgttgccccc gttcagccag
     3121 gcatgtttgc tgatttcaat accaacggta aagatcagca tttgcagcat tcccatcgcc
     3181 gcagaaaccg tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca
     3241 ttcgccagac caataccgaa agcataaata cttaacccgg cagtcatcca taaatacgcg
     3301 tgcgatgaga taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg
     3361 cccataatta tcagcgaacg tacggtgcgg cacgaggtca gacgcgctaa cagcaagtta
     3421 cccgcaatta acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac
     3481 tgctcgccgg taatgatgat aatcggcgac tgggcgatcc acgccagcaa tggcaggcta
     3541 acgaatccca gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag
     3601 tcacgaccga gttctttcag tgacagtttc tcgcctatac gcgtggcggt ttcaggcata
     3661 gctcgttgca gaccgaaaaa ggagatcgct gccaatgcgg caaacaaaac aaacatcccc
     3721 tcccagggca gcacatggat ccacgccgcg cccaccagcg gaccaagtag cggagcaatc
     3781 agcgccacgt tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaagattcc
     3841 tgaattgcgg cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc
     3901 aacagagtga attgttcaat gttttgcgcc agtaatattg ccagacaggt gatgataaac
     3961 cacaccaccc ccgccagcat caccggacgg cgaccaatac gatccgacag cggccccagg
     4021 agccattgta aaaacatccc gcccgccaga tatgcggtca ttgaagtagg aacccaatca
     4081 atgcccgcct gatattgttc caccacggcc aacataccgg gttgaatcat atctgccgat
     4141 ataggttgaa aattcgtaaa gcaccagaca gagagggaaa agtaacgcct gacgtccaag
     4201 cctggcaccg gaagctaatt tattttgcat gcaatttctt cgccaataat aatcgcgcag
     4261 agtttaataa aagcgcagct aacgagaaag cgaattttgt agctgaaacc acggttaagc
     4321 acattcttac attattgcga gtatagctac gctttcttta agttttattt aacctatgcc
     4381 cgttacaatc acccaccgta aacaggccgc ttgagggaaa taagacgatg ccgctttacc
     4441 cagtttaacc tgcactttat tctcaacgac ttgcctgtat tggctccctt ttaatcactt
     4501 tgcgtcggga agttatgctg gaaaatttga atctctctct attctctctt attaacgcga
     4561 cgccagactc ggctccgtgg atgatctcgt tggcgatttt tattgctaaa gatttgatta
     4621 ccgtggtgcc gctgctggcc gcggtgcttt ggttgtgggg gcttacagca caacggcaac
     4681 tggtgataaa aatcgctatc gcgctggcgg tcagcctgtt tgtgtcctgg acgatgggac
     4741 atctttttcc gcacgaccga ccctttgtcg aaaatatcgg ctataacttc ctgcatcatg
     4801 cggcggatga ctcattccca agcgaccacg gtacggtgat tttcaccttt gcactggcat
     4861 ttttatgctg gcatcgcctg tggtccggct cacttttaat ggtgctggcc gtcgtcattg
     4921 cctggtcgcg cgtttatctt ggcgtccact ggccgctgga tatgctcggt ggattgctgg
     4981 caggtatgat tggctgcctt agtgcccaga ttatctggca agcgatgggg cataaactct
     5041 atcaacgtct gcaatcgtgg tatcgcttct gttttgcatt accgatccgc aaaggctggg
     5101 tgcgtgactg atctctgcta aaaagtgtag tattgagcgg ctcgcttcaa taactattca
     5161 gagggattat ggaaacacgt cgcgaagagc gtatcgggca gctgctgcaa gaattaaaac
     5221 gcagcgataa gttacatctt aaagacgccg ccgccctgct tggggtttcg gagatgacga
     5281 ttcgtcgcga tctgaacaac cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc
     5341 tggaaccgca tagtgccagc cattacctgt taagcgatca aaaatcccgc ctggtggaag
     5401 aaaaacgccg ggcggcaaaa ctggctgcga cactggtaga acccgatcag accctctttt
     5461 ttgactgtgg caccaccacg ccgtggatta ttgaagcgat tgataatgaa atccctttta
     5521 ccgccgtttg ttattcgcta aatacctttc tggcgctgaa agagaaaccc cattgccgcg
     5581 catttctgtg cggtggtgaa tttcacgcca gcaacgccat tttcaaaccc atcgattttc
     5641 agcaaacgtt gaataatttt tgcccggata tcgcttttta ttccgcggcg ggcgtgcatg
     5701 tcagtaaagg cgctacctgt tttaatcttg aagagttgcc ggtaaaacac tgggccatgt
     5761 cgatggcgca aaagcatgtg ctggttgtcg accacagtaa atttggcaag gtccgtccgg
     5821 cgcgcatggg tgacctgaaa cgctttgata ttgtggtgag tgattgttgc ccggaagatg
     5881 agtatgtgaa gtacgcgcag acgcaccgta ttaagttgat gtattaatga cgtataaccg
     5941 gatgacgttt cgcgccatcc ggttatcaga agattaagag aaccagctgc cgaaccactg
     6001 atggaatttc atcatcacga aatcccacat ccgaccaaag aatccgccct cttccacatt
     6061 ttccatcacg atcagcggac gctgctcaat ggatttaccg ttaagctgga aatcaatggt
     6121 cccgacaacc tgaccttttt tcagcggtgc ggtaagctgc ggttccgtta acgtgtagct
     6181 cgccttcagg tttttcagct gcccacgcgg tatggtcact gagcccgctt cgcctgcccc
     6241 gagattcact tcgctcttat caccaaacca gacgcgctga gtcacaaagg tggcatcagg
     6301 tttaattggc gtcacggttt caaagaagcg gaaaccccag gtcaataatt tctcagactc
     6361 attaaaacgg atacggtcag ttttcgcccc caacactacg gagattaaac gcatatcgcc
     6421 ctgggtagcc gaagcaacca gattatatcc cgcgcctgcc gtggttcctg tcttcatgcc
     6481 atcaacattc agattgctgc tccacagcag acggttacgg ttaggctgac gaattttgtt
     6541 gaaggtgaac tctttctctt tatgaatggc gtactcttcc ggcacatcgt ggatcaatgc
     6601 tttacccagc aatgccatat cgcgcgcggt actgaactgc cccggcgcat ccaggccgtg
     6661 caccgtctgg aaggtagtgt tggtcagacc cagttttttg gcataaccat tcatcagacc
     6721 aataaatgac tcctggctcc cggcaacgta atcggccagc gcaatacagg cgtcattacc
     6781 ggactggata atcacatctt tgttcaagtc tgccaccgaa acctgatcgc ccggtttgag
     6841 gaacattacc gatgaaccac gcagtgccgg atttcccgtc gcccaggcat ctttaccgac
     6901 cgtcaccata tcggtgagtt taatcttatc ggccttaagc gcctgcccaa ccacatagct
     6961 ggtcatgatt ttagtcaggc tcgcgggatc cagtttctca tccgcattgc cttctgccag
     7021 cactttaccg ctggcgtaat ccattaaaat ccatgcacgc gcatccacgc tcggcgcttc
     7081 aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg aataaaaatg cagaacccgc
     7141 tgcaagacca cgaaggagag aggagtattg cgtcataaat gccacccgag tatccattca
     7201 aaaaaaatac gttatatcgc cgtatgataa gttcggtgat taataacgca cacacaaact
     7261 taaagaaacc gtaagttggt aaagttttta aagtttatgc aatatcctgc cgcttcgctg
     7321 cttctggtgt catttttttg ccagctgttg cttaaaagtt aactttactt caccatcgga
     7381 gtcctgttca gcctgtggct gcctgtcgga aaaggggtga gtatgattac gctgtggggt
     7441 cggaataatt caaccaacgt aaaaaagtat tgctgacgct cgaagaactg gaactacctt
     7501 atgagcaaat tctcgcgggc cgtgagtttg ggataaatca cgatgctgat tttctggcga
     7561 tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga aagtgatctc attctttggg
     7621 aatcaaacgc cattgtccgc tatctggcgg cacagtacgg gcaaaaacgc ctgtggatcg
     7681 actcaccggc acgtcgtgcg gaagcggaaa aatggatgga ctgggcaaac cagacgctca
     7741 gtaatgccca tcgagggatc ctgatgggat tagtcagaac accaccggaa gagcgcgatc
     7801 aggccgccat tgatgccagt tgcaaagagt gcgacgccct gtttgccctg ctcgatgcgg
     7861 aactggcaaa agtaaaatgg ttctctggcg acgagtttgg tgtgggcgat atcgctatcg
     7921 caccgttcat ctacaatttg tttaacgttg gcctgacctg gacaccgcgt ccaaatctgc
     7981 aacgctggta tcagcaactc actgaacgcc ccgcagtgcg caaagtggtg atgattcccg
     8041 ttagctaatt gcgtgggcta actttaagta attccccact ggactcgtcg gtgagaacgt
     8101 ataaataacc gtcgggtcca gtgcgaacat cacgaattcg ctgccctctg tccgttaaaa
     8161 tacggccatc ttctgtcact ttgtcgccgt tgacgctcat cacaatgaca tctttatctt
     8221 tcagcgcgcc aataaataat ttttgctgcc actgggggaa tttatcgctg ttatagaagg
     8281 ccatgccgct cacagcgggc gaatctttcc agtaaaaaac aggttgctcg gtcccggcga
     8341 cgatctcccc tttcgcttcc ggtatcttaa agcctgaata gttgattccc caggttgcca
     8401 gcggccagcc gtagtttttg cctttttgcg ggatattaat ttcatcacca ccgcgcgggc
     8461 catgttcatt cagccacagt gcattactcc acggattcat cgccattcct tgcggattac
     8521 gaatgccata agaccagatc tcggcgcgcg caccggattc ctttataaaa ggattatcat
     8581 ccgggatttc gccctgatcg gtcagacgca ccagtttgcc ctgtaattta tccagatcct
     8641 gcgccgtcgg gcgctgattg ttttcgccca gagcaataaa aagataacct ttaccgtcga
     8701 ataccagccg cccgccaaaa tggttgccgg tagacagttt tggcatctgg cggaagacgg
     8761 tgcggaagtc ggtcactttt gagagatcat cacttaagcg gccataaccc acagcagttc
     8821 cggctttgcc atcatcgcca acttcggaat aacttaacca gatgcggcga gactgagcaa
     8881 aatcaggcgc taaaaccacg tccagcaggc cgccctgccc gtgcgcccaa acgtccggaa
     8941 ctccggaaag cggcgcagat aatccttttc ctgcttgcca gtgacgcaac tcgccgccgc
     9001 gcagagtgat taacataccg tgattatcgg
//
LOCUS       DAJTSL010000161         8736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTSL010000161 DAJTSL010000000
VERSION     DAJTSL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.161"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            244..1155
                     /gene="ttdA"
                     /locus_tag="OO433_003423"
     CDS             244..1155
                     /gene="ttdA"
                     /locus_tag="OO433_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCR6051775.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            1152..1757
                     /gene="ttdB"
                     /locus_tag="OO433_003424"
     CDS             1152..1757
                     /gene="ttdB"
                     /locus_tag="OO433_003424"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6051776.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRGKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1806..3269
                     /gene="ttdT"
                     /locus_tag="OO433_003425"
     CDS             1806..3269
                     /gene="ttdT"
                     /locus_tag="OO433_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6051777.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVFGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(3312..4325)
                     /gene="tsaD"
                     /locus_tag="OO433_003426"
     CDS             complement(3312..4325)
                     /gene="tsaD"
                     /locus_tag="OO433_003426"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6051778.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4562..4777
                     /gene="rpsU"
                     /locus_tag="OO433_003427"
     CDS             4562..4777
                     /gene="rpsU"
                     /locus_tag="OO433_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6051779.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4888..6633
                     /gene="dnaG"
                     /locus_tag="OO433_003428"
     CDS             4888..6633
                     /gene="dnaG"
                     /locus_tag="OO433_003428"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6051780.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6828..8669
                     /gene="rpoD"
                     /locus_tag="OO433_003429"
     CDS             6828..8669
                     /gene="rpoD"
                     /locus_tag="OO433_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6051781.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2109 a         2331 c         2334 g         1962 t
ORIGIN      
        1 cctgcaagtc tttggctaaa ggccagctat tcagcatcgt taattatccg cagttgtgat
       61 aagcgcagtg tatttcgcaa aacattgcca ttccctgctc tggcgcaata ttcattctta
      121 ttttcgcgtt gattcacgaa ctgttaatta catattcacg gtggcaaaaa atataaaacc
      181 acatttttag tggtagttta tggcggtgaa tttttccaga caaatacaaa aactggagtt
      241 gccatgatga gcgaaagtaa taagcaacag gcagtgaata agttgacaga gattgtcgct
      301 aactttaccg ccatgatttc tacccgaatg cctgatgacg tggtggataa actaaaacag
      361 ctaaaggatg ccgaaacgtc gtcgatgggg aaaattatct accatacgat gttcgacaac
      421 atgcaaaaag cgattgacct gaatcgtcct gcctgtcagg acaccgggga gattatgttc
      481 ttcgttaaag tcggttcccg cttcccactg cttggcgagc tgcaaagcat actcaaacaa
      541 gccgtggaag aggcaaccgt caaagcgcca ctacgtcaca atgcggtaga aatttttgac
      601 gaagtaaaca ccggcaaaaa taccggtagc ggcgtaccgt gggtcacctg ggacatcatc
      661 cccgacaatg acgatgcgga aatcgaagtt tacatggcag gtggcggctg cacgctacct
      721 ggccgctcga aagtgttaat gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc
      781 gaaaatatct ccaccctcgc cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc
      841 gccacctcgg tggaaaccgc cgccgtactc tcgcgtaaag ccattttgcg cccgattggc
      901 agccgccatc ccaatccaaa agcggcagaa ctggagctac gcctggaaga aggactcaac
      961 cgtctgggga ttggtccaca agggctgact ggcaacagtt cagtgatggg cgtacatatc
     1021 gaatctgccg cccgccatcc gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg
     1081 catcgtcgcg gcacactgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc
     1141 cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata
     1201 ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgt gacgtttgtc
     1261 accgccgctt gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt
     1321 tccacgctgg ccccatcgtg cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc
     1381 cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tatcgagcag accggcgtga
     1441 aactggtggt tggcaaaggc ggaatggggc cgctgaccga agaaggctgc cagaaattca
     1501 aggcgctaca tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtggaag
     1561 agattgaaga agtgcactgg acagagctcg gaatgccgga gtcgctgtgg gtctgccggg
     1621 gcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaat ctgatagccg
     1681 aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg
     1741 tccattacat caaataaccc tcccggagag gctcgccccc tctccttttt cgcaggcata
     1801 acacgatgaa accttccact gaatggtggc gataccttgc gccgctggcg gtcatcgcca
     1861 ttattgctct aattccggtt cccgcagggc tggagagtca tacctggctc tactttgccg
     1921 tttttactgg cgtgatcgtt ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga
     1981 tggtgggtat ctccatcatc gccatactct ctccctggct gctgttcagc cccgaacagc
     2041 tcgcccagcc aggctttaag ttcaccgcca aatccctctc ctgggcagtt tttggttttt
     2101 ctaattcggt tatctggctc attttcgccg cctttatgtt tggcacaggc tatgaaaaaa
     2161 ccgggcttgg acgccgcatt gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt
     2221 ttctcggcta tgcggtgatg ttctccgagc tgatcctcgc acctgtaaca ccgtccaact
     2281 cggcgcgtgg tgcggggatt atctatccca tcatccgtaa cctgccaccg ctctatcaat
     2341 cacaaccaaa cgacagcagt tcgcgcagca ttggctcgta catcatgtgg atggggattg
     2401 ttgccgactg cgtgaccagc gccattttct tgacggcgat ggcaccaaac ttgctgttaa
     2461 ttggactgat gaaaagcgca tctcacgcca cgctgagttg gggcgactgg ttcctcggga
     2521 tgttgccgct cagtattttg ctggtgctgc tggttccctg gctggcttat gtgctgtacc
     2581 cgccggtact gaagtctggc gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa
     2641 tgggcccgct ctgttcgcgt gaaaaacgga tgctggggct gatggtaggc gcgctggtgc
     2701 tgtggatttt cggcggtgat tatatcgatg ccgcgatggt cggttacagc gtggtggcac
     2761 tgatgctgct tctgcgcatt atcagttggg acgacattgt cagtaataaa gcggcgtgga
     2821 acgttttctt ctggctggcc tcgcttatca ccctcgctac cggactcaac aacactggtt
     2881 ttattagctg gtttggcaaa ctgttagcag gcagcttaag cggttattcg ccaacgatgg
     2941 tgatggtggc gttgattgtg gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg
     3001 cgtatacctc cgccctcgca ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc
     3061 cgctgccggt gttctgccta atggttggcg cggcaattgg tctggggagc attcttacac
     3121 catacgccac cggacccagt ccgatttact acggtagtgg ttatctgcca acggcggatt
     3181 actggcgact gggggcgatt tttgggctga tattcctcgt gttgctggtg attaccggct
     3241 tactgtggat gcccgtggtg ttgctttaag tgaggacggt gccggaaatt gctctccggc
     3301 acccaatagt tttacgcagc cggtaactcc gccagcggcc agcgcggacg cacgctaacg
     3361 ccgagatccg ccgtcgcgcc tgctttaaac cgcaccattc cggcataggc gatcatcgcg
     3421 ccgttatcag tacaaaactc cggacgcgcg tagaacactt cgccgcggcg ttttttcatc
     3481 atttccgcca gcttcgcccg taacgtgcgg ttagcactca cgccgcccgc catgaccagt
     3541 cgcttaaagc ccgtctgatc caacgctcgc ttacacttaa tcatcaacgt atcgaccacc
     3601 gcatcttcaa aggcgcgggc gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga
     3661 atcgtgttcg ccgcaaaggt tttcagacca gaaaagctga aatccagccc cggacggtcg
     3721 gtcatcggac gcgggaaaac aaagcgcccg gcagtaccct gagccgccat tttcgacagt
     3781 aacggtccgc caggataatc cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg
     3841 gcggcatcat cgatagactc gccgagcagc tcgtactgac caatgccagt cacgctgatt
     3901 aactgcgtat gaccgccgga caccagcagc gcgacaaacg gaaattccgg cgggttatct
     3961 tccagcatcg gcgctaacag atgcccttcc atatggtgta ccgggattgc cggaacgttc
     4021 caggcaaacg ccagagaacg ccccacggtt gcgccaacca gcagtgcgcc gactaatcca
     4081 gggcctgcgg tataggccac agcatcaatg tcttttgccg ttaagccaga ctcctttagc
     4141 gccgcctgga tcaacggtac ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg
     4201 acgccgccgt agtcagcgtg caatttcacc tgactataca attggttggc taacaaacct
     4261 ttttcatcgt cgtaaatggc gatgccggtt tcatcgcagg aagtttcaat acccagtaca
     4321 cgcatgactt gttttacctc gctttattac cgcgcagtgt aggaccaatg cgggttgatg
     4381 taaaactttg ttcgcccctg gagaaagcct cgtgtatact cctcaccctt ataaaagtcc
     4441 ctttcaaaaa ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca
     4501 ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca
     4561 catgccggta attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa
     4621 gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa
     4681 accgactacc gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact
     4741 ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg
     4801 atcagaccga gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg
     4861 tttatgaatt gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat
     4921 gatctactgg cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag
     4981 cagggcaaga atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc
     5041 gttaacggtg agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc
     5101 gacttcctga tgaactacga caagcttgag ttcgtcgaaa cggtcgaaga gctggcagca
     5161 atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat
     5221 cagaggcaaa cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta
     5281 caacaacctg ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag
     5341 gttatcgctc gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg
     5401 tttggcggca atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac
     5461 gatcagggac gcagttacga tcgtttccgc gagcgggtga tgttccccat tcgcgataaa
     5521 cgcggtcggg tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg
     5581 aactcgccgg aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg
     5641 cagcaggata acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg
     5701 gcgctggcgc aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc
     5761 gatcacatac aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac
     5821 cgtgcaggcc gtgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac
     5881 ggccgtcagc tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga
     5941 aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt
     6001 ctgtttaaca gcctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg
     6061 agtacgctgg cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt
     6121 cgtcaggaat taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg
     6181 ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg
     6241 cgtatactta tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt
     6301 gagaatctgg atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact
     6361 tgtctctccc agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat
     6421 aatgctgcca cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct
     6481 gagcaaacct tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag
     6541 gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc
     6601 tggacattaa accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga
     6661 tcgggaagcc cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt
     6721 aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg
     6781 tcaatcgccc aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc
     6841 cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg
     6901 ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca
     6961 tccaaatgat caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc
     7021 tgatgctggc tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt
     7081 ccagcgtgga atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa
     7141 tgggcaccgt tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag
     7201 acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc
     7261 tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct
     7321 ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc
     7381 tttcccagga agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca
     7441 gcgccgatga tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac
     7501 gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg
     7561 ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc
     7621 agtttgacta cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac
     7681 gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc
     7741 tgtttaccgg caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc
     7801 cgtggtcgga aaaactgcac gatgtctctg aagaagtgca tcgcgccctg cagaaactgc
     7861 agcagattga agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta
     7921 tgtccatcgg tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac
     7981 gtctggttat ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga
     8041 ttcaggaagg caacatcggt ctgatgaaag cggttgataa attcgaatac cgccgtggtt
     8101 acaagttctc cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg
     8161 atcaggcgcg caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc
     8221 gtatttctcg ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg
     8281 aacgtatgct gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa
     8341 tctccatgga aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg
     8401 ataccaccct cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc
     8461 acgacgtgct ggctggcctg accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta
     8521 tcgatatgaa caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg
     8581 aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg
     8641 aagtgttgcg tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac
     8701 ccggcactag tccctctgca caaacgccac cttttc
//
LOCUS       DAJTSL010000162         8728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTSL010000162 DAJTSL010000000
VERSION     DAJTSL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.162"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            195..1028
                     /gene="ppsR"
                     /locus_tag="OO433_003430"
     CDS             195..1028
                     /gene="ppsR"
                     /locus_tag="OO433_003430"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6051782.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRTANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            1184..2230
                     /gene="aroH"
                     /locus_tag="OO433_003431"
     CDS             1184..2230
                     /gene="aroH"
                     /locus_tag="OO433_003431"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6051783.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARTSHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            2362..2553
                     /gene="hemP"
                     /locus_tag="OO433_003432"
     CDS             2362..2553
                     /gene="hemP"
                     /locus_tag="OO433_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6051784.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2557..3993)
                     /locus_tag="OO433_003433"
     CDS             complement(2557..3993)
                     /locus_tag="OO433_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6051785.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNIELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLIQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(4056..4769)
                     /gene="ydiV"
                     /locus_tag="OO433_003434"
                     /pseudo
     CDS             complement(4056..4769)
                     /gene="ydiV"
                     /locus_tag="OO433_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            complement(5015..5479)
                     /locus_tag="OO433_003435"
     CDS             complement(5015..5479)
                     /locus_tag="OO433_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6051786.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDKLLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(5557..6306)
                     /gene="btuD"
                     /locus_tag="OO433_003436"
     CDS             complement(5557..6306)
                     /gene="btuD"
                     /locus_tag="OO433_003436"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6051787.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLILTI"
     gene            complement(6306..6857)
                     /gene="btuE"
                     /locus_tag="OO433_003437"
     CDS             complement(6306..6857)
                     /gene="btuE"
                     /locus_tag="OO433_003437"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6051788.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(6920..7900)
                     /gene="btuC"
                     /locus_tag="OO433_003438"
     CDS             complement(6920..7900)
                     /gene="btuC"
                     /locus_tag="OO433_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6051789.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISLGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELLIGVVTATLGAPVFIWLLLKARR"
     gene            complement(8109..8438)
                     /locus_tag="OO433_003439"
     CDS             complement(8109..8438)
                     /locus_tag="OO433_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051790.1"
                     /translation="MNDDLERRVSCLESDVTEIKNNLITLTTRSESFATKSDVLEIRE
                     GLRLEMAESRQSLKSEMADLRQSLKVEMAEHRTELQKSFANQTWLLTGIVLSAMAVLV
                     AVVTVIK"
BASE COUNT         2303 a         2309 c         1996 g         2120 t
ORIGIN      
        1 gttgcgcaaa cctgctctat tcgacaattt ccacataaac catcggtata aacgaaacca
       61 taaaaatgaa atgctgtttt cataaaaaat aaaattgaag gttcatttta taaaccagac
      121 attacctcct acgcttctgt gcgtttttaa tttatgcttt catagaatta tgtctgcatc
      181 acgggaagaa caaaatggat aatgctgttg atcgccacgt tttttatatt tctgatggta
      241 cggcaataac tgcggaggta ttaggacacg cagtaatgtc acaatttccc gtcactatca
      301 gcagcattac gctgccgttt gtcgaaaatg agagccgtgc acgggcagtg aaggatcaga
      361 ttgacgcaat ttatcaccag acaggtgtgc gcccgctggt cttctactcc atcgtgttgc
      421 cggagattcg cgccatcatc ttgcaaagtg aaggcttttg ccaggatatc gttcaggcgc
      481 tggttgcccc gctacaacaa gagatgaaac tggatccaac gccgattgct catcgtaccc
      541 atggccttaa ccctaataat ctcaataaat atgatgcgcg cattgcggcg attgattaca
      601 cccttgccca cgatgacggc atttcgttgc gcaatctgga tcaggctcag gtgatcctgc
      661 tcggtgtttc tcgctgtggt aaaaccccca ccagtctgta tctggcaatg caatttggta
      721 tccgcacggc aaactacccc tttattgccg acgatatgga taatctggtg ctacccgcgt
      781 cgctcaaacc gcttcagcat aaattgttcg gcctgactat cgacccggaa cgtctggcgg
      841 cgattcgcga ggaacgtcgg gagaacagtc gctatgcctc gcttcgtcag tgcagaatgg
      901 aagtcgcgga agtggaagcc ttgtaccgta aaaatcagat cccgtggatt aacagtacca
      961 attattcggt agaagagatt gccaccaaga tcctcgatat catgggcctt agtcgccgaa
     1021 tgtactagag aactagtgcg ctggtttgtt ttttgttatc atgctaacca cccggcgagg
     1081 tgtgacacac ctcgcacttg aaatcagctg cgattggttt atcgtgatgc gcatcacttc
     1141 ccggtagtcc tgccgtagaa gcaacaaatt tctgagactt gtaatgaaca gaactgacga
     1201 actccgtact gcgcgtattg agagcctggt aacgcccgcc gaactcgcgc tacggtatcc
     1261 cgtaacgcct ggcgtcgcca cccatgtcac cgactcccgc cgcagaattg aaaaaatact
     1321 gaatggtgaa gataagcgac tgttggtcat tattggcccc tgctcgatcc acgatctcac
     1381 cgctgcaatg gagtacgcca cccgtctgca gtcgctgcgc aaccagtacc agtcacggct
     1441 ggaaatcgta atgcgcacct attttgaaaa accacgaact gttgtcggct ggaaagggct
     1501 aatctccgat ccggatttaa acggcagcta tcgggtaaat cacggtctgg agctggcgcg
     1561 caaattactt ttacaggtaa atgagctggg tgtccctacc gcgactgagt ttctcgatat
     1621 ggtgaccggt cagtttattg ctgatttaat cagttggggc gcgattggcg ctcgtactac
     1681 cgaaagtcag atccaccgcg aaatggcttc agcactctcc tgtccggtag gttttaaaaa
     1741 tggtaccgat ggcaacacgc ggattgccgt ggatgctatc cgcgcagccc gcaccagcca
     1801 tatgttcctc tcgccagaca aaaatggtca gatgaccatc tatcagacca gcggcaaccc
     1861 gtatggccac attattatgc gtggcggcaa aaaaccgaat tatcatgccg atgatatcgc
     1921 cgcagcctgc gatacgctgc acgagtttga tttacctgaa catctggtgg tggatttcag
     1981 ccacggtaac tgccagaagc agcaccgtcg ccagttagaa gtttgtgagg atatttgtca
     2041 gcaaatccgc aatggctcta cggcgattgc tggaattatg gcggaaagtt tcctgcgcga
     2101 aggaacgcaa aaaatcgtcg gcggtcagcc gctcacttac ggtcaatcca ttaccgaccc
     2161 gtgtctgggc tgggaagata ccgaacgcct ggtcgaaaaa ctcgcctctg cggtagatac
     2221 ccgcttctga atgcgtgccc atgcctgatg ggatgggcat ttctgcgcaa cttgttgtct
     2281 tctcaacaca ttactgcttg ctctggcctg ccataatatt gataataaga atcattgtta
     2341 tatcaattat tattaatttt tatgcgttat acggatagca gaaaactcac gcctgaaacg
     2401 gatgccaatc acaagaccgc ttccccgcag cccatccggc gaattaccag ccagacactg
     2461 ttaggtccgg atggcaaact gattatcgat catgacgggc aagaatatct gctccgtaaa
     2521 actcaggctg gcaagctgct gttaactaag tagcctttaa ctcgagcagc tgacttccag
     2581 ccgtttaccc cagtcaggtg gacgactgac atagtcatca tctctgtcgc tgaaaggatt
     2641 tcgcaacgcc tcatgcaggc ggtgcaattc catcatatca cccttttctg ccgcttcaat
     2701 cgcccgttgc gccaaccagt tgcgcaatac cagagctggg ttaacgcttt gtatcagttg
     2761 ctgacgctca ctatcgctaa cctcgtcttg ttgcaaacgc ccccgataac gggcaaacca
     2821 gtcatcaaat gccgcacgat caataaactc atcacgtagc ggtgacgccg cgctgtgctg
     2881 ctcggtcaga ctcagcatgc ggaatgtgcg ggtataatcg ctgcgctctc gcgccatcag
     2941 actgaataat tcattcagta gcgcgttatc ctctttttgc tccgtcatga agcccagttt
     3001 ctgccgcatc cgttgtccat aatgcgtcaa caaaacctgc tgatagctgt ccagggcctc
     3061 attcagggca tctacggcaa caaatggtga caatgtctgc gccagacgct gtaaattcca
     3121 caacgcgaca gcaggttgat tatcaaagct ataacgccct tgatgatccg agtgattaca
     3181 aataaaaccg ggttcgtaat catcaagaaa accaaacggc ccgtaatcaa gcgtcagtcc
     3241 cagcagcgac atgttgtcgg tattcatcac cccatgtgca aagccgaccg tctgccattg
     3301 ggcaattaac gacgcggtac gtgcgacaac atcgctaaac cagagacggt atttgtcctc
     3361 atcatcttca agatgtgacc agtaatgacg aatagcaaag tcagctaact gacgaacctt
     3421 ttccgactcg cggcggtagt aaaaatgttc gaaatgacca aagcgcagat gacttggtgc
     3481 cacacgcatc agcatcgcgc ctggctccgc cgtttcccga tacactggcg aatcgctggt
     3541 gacgatactt aacgcgcggg tcgtcggaat gcccagataa tgcatcgcct cactggcgag
     3601 actttctcgt atcgtcgaac gtaaaaccgc ccgtccatca cccattcgcg aataaggcgt
     3661 caggccagca cctttcagat gccagtccat tgtagtgcca tcagcaagca gttgttcgcc
     3721 gagtaaaatg ccgcgcccat cacccagttg gcccgcccag acgccgaact gatgaccact
     3781 gtaaacctgc gccagtggtg acataccagg cagtagggct tcgccgcccc agacacctgc
     3841 gccattttta aacagcgacg atggaatact cagcgtgtta gccagttcga tattatgcca
     3901 aatcagccgg gcattattta aaggcgtagg ggaaagtgct gtataggttt ctggcaattc
     3961 atcgcgccag cgggtaacaa aagacagggt catagatcct cctgtttgat agtgtagacg
     4021 gttactctcg tcttaaacac cagcaaacag aagggttatc gctgaaccaa cgtcgttatc
     4081 tgatttatgg gaacggcagg ccacaggcat ccctggagag catggaaatc gaagggggta
     4141 atctgtgcca atatttctgc cgtatcaatg cctcccgcaa tgatgcaatt gcaacaaggg
     4201 gaaatttgcg cctagatcgc acgaataaac ggttcgaagg agcggtgcgt gatttgttgc
     4261 tgaataaaac ttttatccag catgacgcgg gtgaaaagac catcaaaaac cgctttcatt
     4321 gtgctattgc ctgctcccaa attacctaac acaaggggat acacttgcga taaggataaa
     4381 agaccgcgat tatcttttcc ttcattgaga tgcgggtaat tctcattaat caacagttca
     4441 ataaacggat attttaatag ttcgcctgcg taattatccc tctctaataa taatgtagcc
     4501 acttgtggcg ttaaattaag ccaggcaaag agtttatgtt gaataaaaaa atgttggcat
     4561 gattttagta attccaactg ctctgaaaat aattgccaat gttgctcctc ggtaagttgc
     4621 gctataaccc gactcgtcgg gatacgcacc gtgccatctt cactggagaa atgggtaatt
     4681 aactcaacgc cgacaaggac ttgctggtta tctcttatcg gcaggaaata gcaatcagaa
     4741 tgataaagat tctccaaaaa aatcttcatg gtaaccgtcc ctctcgaagg atgtttttca
     4801 gtatccggct aaaagggata aggccataag acataacaat tacagaagga gtaactttca
     4861 tttgttccat gttaaccact ttttcagggg tcttttttta gattatcctg attataaacg
     4921 aataatccat ttacggaatt tttgtctgca aaatactact gtattcagga gtaaatggac
     4981 ggcgatgtat catcgccgtc catttttcga catactagat tcgtctcgcc tgccagaaat
     5041 ttttctgcca atagacatta tcaagtgagg aacgcatcac tcccttgctg gtagaggcgt
     5101 ggataaattg gttgttggta tcgtaaatac ctacatgcaa accattttgt ccggaccccg
     5161 ttttgaaaaa gaccaggtca cctggcagca acttgtcttt atcaatttgc gtgccaatag
     5221 aggcttgttg tttggtttca cggggcagct gcaaatcgaa acgatcgcgc atcgtcacaa
     5281 ccacaaatcc cgaacagtcc acaccgcgcc gcgtcatgcc accataacga tacggcgtgc
     5341 catgccagct ttgtagctgg tcgttcaaac cggcaataac ggtaatcgaa tcagaaagtc
     5401 tggcatttgg cggcggtgct ttatggtggc tacacccggc cagaaacagt gctgtgatca
     5461 aaataaggca gaaacgcatt ccgtacggtt cctctgtttt ttattcttgc attaatttag
     5521 cgtcgtaatt acccgatttt caagatacta atgaaatcag atggtcaaaa tcagcattct
     5581 gtgaccttcg atatccagac ggcgaaaatt catcccatag gcctgcgcca gatttggcgg
     5641 cgtgagcacc tcttctctgc gtccactggc cagcattttt ccacctttta gcaaccacgc
     5701 ccgatgcgca tgacgcaacg tgtggttgag atcgtgactg ctcatcacaa tcgccagtcc
     5761 ttgctgacac agcgcgctca gaattttgtc taacgcactt tgttgcgcaa catcaagact
     5821 gttcatcggc tcatcaagaa gcagcaattg gcctgcggga ttggcttgtg gtgtgatttg
     5881 caacaccacc gcagcaagac gtacgcgttg ccattcaccg ccggaaagtt gattggtgct
     5941 acgaccgagt ttgtcatcaa gagccagcgc ccctgcgaca tcattcagta gttcggtacg
     6001 cgttttatcg tgctgatgca gtgtcaggta gtgccagacc ggcattgcaa acggcggcgt
     6061 ctgctgttgt gaaagatagg cgcgatgcag cgcgagtttt gttgcggacc atgcttccag
     6121 gggttgcccc gcgaactgaa tgcttccctt accgctggtc attccggcca ttcgtgcaag
     6181 caaggtactc ttacccgcgc cattcggccc caccaggtgc aggatctccc cagcccgaac
     6241 ctcgccagaa agtggcccca ggcgggtaga ttccgcaaca tcctgtaact gcatcacaat
     6301 agacattatt ttgccaacgc cagtttaatg ctttccatca caatggggtc ttccggcgtc
     6361 atatccgggg aaaaacgctg gatgaccaat ccgtccctgc caactaaaaa tttctcaaaa
     6421 ttccataaaa tatcatcagg gtacagcggt gcacgacctt tgctgaccat acgggcatag
     6481 aatccgctct cttccggcgc gactgcggtc ggcgctgcgg caatcaattt ttgatacagc
     6541 ggatggcgtc cttcgccgtt aacttcaatc ttactgaaca tcgggaacgt caccccccat
     6601 gtggtggtac agtaagtttt aatctcttca tcgctgcccg gttcttgttc cagaaactgg
     6661 ttgcacggga atcccagcac cacaaaacct cgatcggccc aggctttctg aatattctcc
     6721 aactgctcat attgcggcgt taagccacac tttgaggcga cattgacaat caacagcaca
     6781 ttaccggcgt acttctccag cgtggtcact tcaccgtcga tatctttcac tacggtcgtc
     6841 agaatggaat cttgcatcgt ttctcctggg tgtggtcagt aaaaatctta gcttttaatc
     6901 atagaccgtc tttttgcggc taacgtcttg cttttaacaa taaccagata aacaccggcg
     6961 cacccaacgt tgcggtgacc acgccaataa gcagctctgc ggcagctaat gccaggcgcg
     7021 ctacaatatc ggccagcagc aatgcgctcg cccctgccag cgcgcagccg ggaagtaata
     7081 cgcgatgatc ggttaaacca cacaaccgaa gaatatgtgg gatcaccaga ccaataaagc
     7141 cgatagcacc cgccagcgcc acactgacgc caaccatcca gccggtcgcg gccaccagca
     7201 cattgcgcca gaaccacagg ggtaaaccca gttgccgcgc cgagatctcg ccaagtgcta
     7261 acatattcat cggcctggac tgacaacaga tccacaacaa cacggggatc aatgccagca
     7321 tcagccagct ttgccgccag tctacgccgc caaaaccgcc catcatccag tacatcagct
     7381 gacgtaaatc aacagaggtg gaaaagtaga tagcccacgt cattagtgcg ctacagataa
     7441 tccctaatgc aacgccagcc agcaataacc gactggtcga aagatgacga cgggcgaaac
     7501 gtaagagtat taaagtaatg ataagcgcgc cagcaatcgc acacagccct agcgcccagt
     7561 tggggagttg cccttgccca agcaataccg cggcgataag ccccacgcct gcgccgttag
     7621 agacgccaag tagtccaggt tctgccagag gattttcaaa caacgcctgc attacagcgc
     7681 cggatatagc cagcgccgca ccaaccaaca atacagccag cgtacgtggc aggcgaattt
     7741 gccagacgaa cagttcgcca cgaggagtaa accagtcacc tagcgagatc cattgttcac
     7801 cggcgcaaag gcttaaaaga agcgccagca gcatcaaaac tgacaggcat aataaccagc
     7861 gaatattttg tcgctgttgt tggcgggcaa gtgtcagcat ggtatccgtt ctgctgaagt
     7921 gtcatggcgt tgattttacg gtgactcttc gacagtgaaa agaaaaaagg ccgcagagcg
     7981 gccataaaca caagtaaaaa tcaacaagtt agataattat caaagactta cagacacaca
     8041 aaaacacagc caaccacaac aaataacagg gatgtggtca ctttgtggat catatcgccg
     8101 ccataaattt acttaataac agtaaccaca gccacaagca cggccatcgc agacaggaca
     8161 atacctgtga gtaaccaagt ctgatttgca aatgatttct gaagttcagt acgatgctca
     8221 gccatctcaa ctttcagaga ctggcgtaaa tcagccatct cagacttcag ggattggcgc
     8281 gattctgcca tctcaagcct caacccttcg cgaatctcca gtacatcaga tttggtagca
     8341 aaggattcgc ttcttgtggt gagagtaatc aggttgtttt tgatttcagt cacatcactt
     8401 tccaggcaac tgacccgccg ttcaaggtcg tcattcatgc catcacctcc atcattacca
     8461 cttcctgtta cttggttgtt ggatacatta taggcattct ctttcttttt tctgaaccgc
     8521 gaaatatgtg caacattctc tgccatcact tctcatcacc ctgcttgcct acccagttca
     8581 taaaggcccg ggccgagtaa cgatgaacaa acccacatct gttacaggta agtcggaatt
     8641 catagttatg gatattttcc cgatcatgtc cctcatatcc cggataccca gtaaatggac
     8701 cgatgtaatc aagcataaca aaagtgcc
//
LOCUS       DAJTSL010000163         8725 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTSL010000163 DAJTSL010000000
VERSION     DAJTSL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.163"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1217)
                     /gene="rhsC"
                     /locus_tag="OO433_003440"
     CDS             complement(<1..1217)
                     /gene="rhsC"
                     /locus_tag="OO433_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HCR6051791.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGKFSGEITGVTDGAGRHFQLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTQGEAG"
     gene            1445..2053
                     /locus_tag="OO433_003441"
     CDS             1445..2053
                     /locus_tag="OO433_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6051792.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            2151..3542
                     /gene="selA"
                     /locus_tag="OO433_003442"
     CDS             2151..3542
                     /gene="selA"
                     /locus_tag="OO433_003442"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6051793.1"
                     /translation="MTTETRSLYSQLPAIDRLLHDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3539..5383
                     /gene="selB"
                     /locus_tag="OO433_003443"
     CDS             3539..5383
                     /gene="selB"
                     /locus_tag="OO433_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6051794.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFSDEPWW
                     VRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            5573..6724
                     /gene="yiaY"
                     /locus_tag="OO433_003444"
     CDS             5573..6724
                     /gene="yiaY"
                     /locus_tag="OO433_003444"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6051795.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAYQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            6889..8427
                     /gene="aldB"
                     /locus_tag="OO433_003445"
     CDS             6889..8427
                     /gene="aldB"
                     /locus_tag="OO433_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR6051796.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT         1992 a         2264 c         2469 g         2000 t
ORIGIN      
        1 ccggcttcac cctgcgtgtg cagcacttca cggcggttca ggctgtcggt gatggtgatg
       61 cggtccttct catactgata cgtgtagctt aagcctgccg ggttaagctg ttctgtcacc
      121 cgcccgtcgc tgtcgtaacg gtaacggatt tccggccggc ccgtgtgacg gtgcgccacc
      181 atccggcccc ggtatttatc atcgtaagta aagctacgca cctgtgtatt gctgcggtca
      241 tacactgccg ccagttcgcc gcgcggcgtc cagccatagc gcaccagcgg cgcggcaggt
      301 aaattctccg ggtattccgg gtcgtgcgtc agccacacgg cagacagacg gatgccgttg
      361 tcccggccat attcggtgta acccggcagg gtatcaggaa aagcggacgg ttccgtcccg
      421 ccggaaatgg cctgctgccg ggcttcttct gcccgctgcg cctgcgtggt cagtaccagt
      481 tggaagtgac gcccggcacc atccgtcacg ccggtgattt cgccgctgaa tttaccggcg
      541 gcttcgcggt ggaacgtctg tgtgcgcccg aagcggtcca ccagcccggt cagtacccgg
      601 tacggcggca gcggcgcagg cagcacctca tccgcttccg gcacccgctc acaccagccg
      661 agcagccacc acggcccctg cggactgttt gtcgccagat aacgatgcgg acttaagcgg
      721 agttcttccg gcagcgcctg ccagagtgcg gccagccggt gaccttcatc cagtttcgcc
      781 acgccgccgc gcaccagcca cagtgactcg ctgcggctgt aaccgtcctc accgggaaac
      841 aggtgctcaa aatacaggct tctgccgccg ttatcactga gtatcagtgt gttatcgcgc
      901 agctgtaagc ggatatccgc aggcattttc cagccggggc cgaggctccc caccggcgcg
      961 ggcgtttttg tccggtaact gctgtaggtg cgggagagga tgaacggcag cgggccgggc
     1021 agggcgatgt cggtttcacc gggaaggacc tttgcaccga gcaggggatt gaccggatgg
     1081 ccggacgtca ctccgccggg gcacaccgaa caggccacgc cggtgggggc accaatgcgc
     1141 accccggctg aaccctgaac aatgctaccg ccatactgcg tcatgtcgcc ctgacgcgct
     1201 gccggttttc cgctcatttc tcatccttgt tttatcgggt tatttgtctg ctgacaggca
     1261 aaaccctaca gatttataag aaagcctgaa gtgagatatc catcagagtg aaataattag
     1321 gaaaatattt atatcaccat gataaatatg tgatttattt ggaagtaaca aaccatcaac
     1381 attttctgcc ctttttccca tcaccaggcc atactgatat ttttcctcgc acaaaggact
     1441 cggcatgaaa ctcgtcggta gctacaccag cccgtttgta cgcaaacttt ctattctgtt
     1501 gttagaaaag ggcataactt tcgaatttat taatgaactg ccctataacg cggacaacgg
     1561 cgtggcgcaa tttaacccgt taggaaaagt gccggtgctg gtgaccgaag agggcgaatg
     1621 ctggtttgat tcgccgatca tcgctgaata tattgagtta atgaatgtcg ctccggcgat
     1681 gttgccgcgc gatccgctgg agtcgttgcg ggtgcgcaaa attgaggcgc tggcggatgg
     1741 cattatggat gccgggctgg tatcggtgcg tgaacaggcg cgtccggcgg cgcagcagtc
     1801 tgaagatgaa ttgttacgcc agcgggagaa aatcaaccgc agtctggatg tgctggaagg
     1861 atatctggtc gatggcacac tcaaaaccga tacggtcaat ctggcgacta tcgccattgc
     1921 ctgtgctgtc ggatatctca atttccgccg cgttgcgcca ggttggtgtg tcgaccgccc
     1981 acacttagtc aaactggtcg aaaacctgtt tagccgcgaa agttttgctc gcaccgaacc
     2041 gccaaaggct tgatgcgcga tatgtcctcc tgacccatct cacgttacaa tccgtggtta
     2101 tgttaaacgc ccttctccat gtgagagggc cttgattagc caggtttcct atgacaaccg
     2161 aaacgcgttc cctctatagt caacttccgg ctattgatcg cttattgcac gatagctcct
     2221 tcctttcttt gcgtgatact tatggtcaca cccgcgtggt ggaattgttg cgtcagatgc
     2281 tcgacgaagc gcgagaagtg attcgtgaca gccagacgct tcctgcgtgg tgtgaaaact
     2341 gggcgcaaga agtcgatgcc cggttgacga aagaggcgca gagcgcgctg cgtccggtga
     2401 tcaacctgac gggaaccgtg ctgcatacca accttggacg agctttacag gcggaagccg
     2461 cggtggaagc cgttgcgcag gctatgcgtt cgccagtgac cctcgagtac gatctggacg
     2521 acgctggacg cggacatcgc gatcgggcgc tggcgcaact gctgtgccgt attacggggg
     2581 cggaagatgc ctgtatcgtc aataacaatg cggcggcggt gttattgatg ttggcggcca
     2641 ctgccagcgg gaaagaggtg gtagtatctc gcggcgaatt ggtggagatt ggcggcgcgt
     2701 ttcgtattcc cgatgttatg cgtcaggcag gctgcaccct acacgaagta gggaccacca
     2761 accgcacgca cgcgaatgat tatcgtcagg cggtgaatga aaataccgca ctgttgatga
     2821 aagtacatac cagtaactac agcattcagg ggttcaccaa agcgatagat gaagcggaac
     2881 tggtggcgct cggcaaagag ctggatgttc ccgtagtgac tgatttaggc agtggctcgc
     2941 tggtcgatct tagccagtac ggtttgccga aagagccaat gccgcaggag ttgattgcgg
     3001 cgggcgtcag tctggtgagc ttctccggcg acaagttgtt aggcgggccg caggcaggga
     3061 ttatcgttgg taaaaaagag atgatcgccc gcctgcaaag ccacccgctg aagcgtgcat
     3121 tacgcgcgga taaaatgacc ctcgcggcgc tggaagccac gttgcgtctt tatttacacc
     3181 ctgaagctct gagtgaaaaa ttaccgaccc tgcgcctgct tacccgcagc gcagaggtca
     3241 ttcaaatcca ggcacaacgt ttacaggctc cccttgccgc acattacggc gcggagtttg
     3301 cggtacaggt tatgccatgt ctttcgcaga ttggcagtgg ttcgctgccg gttgatcgcc
     3361 tgccgagcgc ggcattaacg tttacacccc atgatggacg cggtagccac cttgagtcat
     3421 tagccgcccg ctggcgtgaa ttgccagtgc cggtgattgg tcgtatttat gacggacgat
     3481 tgtggctgga tttacgctgc cttgaagatg agcaacggtt tttggagatg ttgttgaaat
     3541 gattattgcg actgccggac acgttgacca cggcaaaaca accttattgc aggcgattac
     3601 tggcgtaaat gctgaccgtc tgccggaaga aaaaaagcgc ggcatgacca tcgatctcgg
     3661 ctatgcctac tggccgcagc cggatggtcg cgtgcctagt tttatcgacg tacccggtca
     3721 tgaaaagttt ctttccaaca tgctggcggg cgttggtggt atcgatcacg cgctgttggt
     3781 ggtggcgtgc gatgacggcg tgatggcgca gacccgtgag catctggcga ttttgcagct
     3841 gaccggtaac ccgatgctga cagtggcgct gaccaaggcc gatcgcgtgg acgaagcgcg
     3901 tgttgatgag gttgaacgcc aggtaaagga ggtgctgcgg gaatacggtt ttgctgaggc
     3961 aaaactgttt atcaccgcag caaccgaagg tcggggaatt gatgccctgc gcgagcatct
     4021 gcttcagtta ccaggacgtg aacacgccag ccaacatagt ttccgcctcg cgatcgaccg
     4081 cgcatttacc gtaaaaggtg ccgggctggt cgtcaccggt acggcgttaa gcggggaagt
     4141 gaaggtaggc gattcactct ggctgactgg tgtaaataaa ccgatgcgtg tacgtgcgct
     4201 gcatgcacaa aaccagccaa cagaaaccgc ccatgccggg cagcgtatcg cgcttaacat
     4261 cgcgggtgat gcggaaaaag agcagattaa ccgtggcgac tggctgcttg ccgatgcgcc
     4321 gccagagccg ttcacacggg tgattgtcga gcttcaaacc catacaccgc tgacccagtg
     4381 gcagccgctg catattcacc acgccgccag ccacgtcacg ggacgcgttt cactgctgga
     4441 agataacctt gctgaactgg tcttcgacac cccgttatgg ctggcagata acgaccgcct
     4501 ggtattgcgc gatatctctg cccgcaacac gctggccgga gcgcgcgtcg tgatgcttaa
     4561 cccgccgcgt cgcggtaaac gtaaaccaga gtatctgcaa tggctggctt ccctcgccag
     4621 ggcacagaat gatagcgaag cgttagccgt tcacctgcaa cgcggcgcgg ttaatcttcc
     4681 tgatttcgcc tgggcgcgcc agctcaacgg cgaagggatg cgcgaactgc tgcaacagcc
     4741 tggctatatt caggctggtt atagcttgtt gaatgcgccg gttgccgctc gctggcaacg
     4801 gaaaattctc gacacattag cgacttatca tgagcaacat cgcgatgaac ctgggcctgg
     4861 gcgcgaacgt ctgcgacgta tggcgttgcc aatggaagat gaagcgctag tactgttgct
     4921 gattgaaaag atgcgcgaaa gcggcgacat cctcagtcat cacggttggc tgcatctgcc
     4981 ggatcacaaa gcgggcttca gcgaagagca gcaggccatc tggcaaaaag cagagccgct
     5041 gtttagtgac gagccgtggt gggtgcgtga tctggcaaaa gagacgggta ccgacgagca
     5101 ggcaatgcgc ttgacgctaa gccaggcggc gcagcaagga ataattaccg cgatcgtcaa
     5161 agatcgttat taccgtaacg atcggattgt cgaatttgcc aatatgatcc gcgatctcga
     5221 tcaggagtgt ggttcaacct gcgcggcgga tttccgcgat cgcttaggcg taggccgaaa
     5281 gctggcaatt cagattctgg aatattttga ccgcattggc tttacgcgtc gtcgtggaaa
     5341 tgatcattta ttgcgcgacg cattattatt tccggaaaaa taaggaaatg attaattaat
     5401 ttttaaaata aattaataca aatttcttat gattttaaaa aaagcacatt gtttaatgaa
     5461 tacaatgtgc tttttattag attaattttg ataattaata ataatgatca tgttttcgaa
     5521 tgaaaatcct cagtaagctt cccacccttt tttacacttt caggagtgtg ttatggcatc
     5581 ttcaactttc tttattcctt ctgtgaatgt catcggcgct gattcattga ctgatgcaat
     5641 gaatatgatg gcagattatg gatttacccg taccttaatt gtcactgaca gtatgttaac
     5701 gaaattaggt atggcgggcg atgtgcaaaa agcactggaa gaacgcaata tttttagcgt
     5761 tatttatgat ggcacccaac ctaaccccac cacggaaaac gtcgccgcag gtttgaaatt
     5821 acttaaagag aataattgcg atagcgtgat ctccttaggc ggtggttctc cacacgactg
     5881 cgcaaaaggt attgcgctgg tggcagccaa tggcggcgat attcgcgatt acgaaggcgt
     5941 tgaccgctct gcaaaaccgc agctgccgat gatcgccatc aacaccactg cgggtacggc
     6001 ctctgaaatg acccgtttct gcatcatcac tgacgaagcg cgtcatatca aaatggcgat
     6061 tgttgataaa catgtcactc cgctgctttc tgtcaatgac tcctctctga tgattggtat
     6121 gccgaagtca ctgaccgccg caacgggtat ggatgcctta acgcacgcta tcgaagcata
     6181 tgtttctatt gccgccacgc cgatcactga cgcttgtgca ctgaaagccg tgaccatgat
     6241 tgccgaaaac ctgccgttag ccgttgaaga tggcagtaat gcgaaagcgc gtgaagcaat
     6301 ggcttatgcc cagttccttg ccggtatggc gttcaataat gcttctctgg gttatgttca
     6361 tgcgatggcg taccagctgg gcggtttcta caacctgcca cacggtgtat gtaacgccgt
     6421 tctgctgccc catgttcagg tattcaacag caaagtcgcc gctgcacgtc tgcgtgactg
     6481 tgccgctgca atgggcgtga acgtgacagg taaaaacgac gcggaaggtg ctgaagcctg
     6541 cattaacgcc atccgtgaac tggcgaagaa agtggatatc ccggcaggcc tacgcgacct
     6601 gaacgtgaaa gaagaagatt tcgcggttct ggcgactaat gccctgaaag atgcctgtgg
     6661 ttttactaac ccgatccagg caactcacga agaaattgtg gcgatttatc gcgcagcgat
     6721 gtaatcatca tttccacaac ggctggcaaa ttgtcagccg ctttttcaac tatctctgta
     6781 acccttgccc gtaaattcgt gatagccgtc gtaaagctgt taccgactgg cgaagatttc
     6841 gccagtcacg tctacccttg ttatacctca caccgcaagg agacgatcat gaccaataat
     6901 cccccttcag cacagattaa gcccggcgag tatggtttcc ccctcaagtt aaaagcccgc
     6961 tatgacaact ttattggcgg cgaatgggta gcccctgccg atggtgagta ttaccagaac
     7021 ctgacgccgg tgactgggca gctgctgtgc gaagtggcgt cttcgggcaa acgagacatc
     7081 gatctggcgc tagatgctgc gcacaaagtg aaagataaat gggcgcacac ctcggtgcag
     7141 gatcgcgcgg cgattctgtt taagattgcc gatcgaatgg aacaaaacct cgagctgtta
     7201 gcgacagctg aaacctggga taacggcaaa cccattcgcg aaaccagtgc tgccgacgtg
     7261 ccgctggcga ttgatcattt tcgctatttc gcctcgtgta ttcgggcgca ggaaggtggg
     7321 atcagtgaag ttgatagcga aaccgtggcc tatcatttcc atgaaccgtt aggcgtggtg
     7381 gggcagatta tcccgtggaa cttcccgctg ctgatggcga gctggaaaat ggctcccgcg
     7441 ctggcggcgg gcaactgtgt ggtgctgaaa cccgcacgtc ttaccccgct ttctgtactg
     7501 ctgctaatgg aaattgtcgg tgatttactg ccgccgggcg tggtgaacgt ggtcaatggc
     7561 gcaggtgggg aaattggcga atatctggcg acctcgaaac gcatcgccaa agtagcgttt
     7621 accggctcaa cggaagtggg ccaacaaatt atgcaatacg caacgcaaaa cattattccg
     7681 gtgacgctgg agttgggcgg taagtcgcca aatatcttct ttgctgatgt gatggatgaa
     7741 gaagatgcct ttttcgataa agcgctggaa ggctttgcac tgtttgcctt taaccagggc
     7801 gaagtttgca cctgtccgag tcgtgcttta gtgcaggaat ctatctacga acgctttatg
     7861 gaacgcgcca tccgccgtgt cgaaagcatt cgtagcggta acccgctcga cagcgtgacg
     7921 caaatgggcg cgcaggtttc tcacgggcaa ctggaaacca tcctcaacta cattgatatc
     7981 ggtaaaaaag agggcgctga cgtgctcaca ggcgggcggc gcaagctgct ggaaggtgaa
     8041 ctgaaagacg gctactacct cgaaccgacg attctgtttg gtcagaacaa tatgcgggtg
     8101 ttccaggagg agatttttgg cccggtgctg gcggtgacca ccttcaaaac gatggaagaa
     8161 gcgctggagc tggcgaacga tacgcaatat ggcctgggcg cgggcgtctg gagccgcaac
     8221 ggtaatctgg cctataagat ggggcgcggc atacaggctg ggcgcgtgtg gaccaactgt
     8281 tatcacgctt acccggcaca tgcggcgttt ggtggctaca aacaatcagg tatcggtcgc
     8341 gaaacccaca agatgatgct ggagcattac cagcaaacca agtgcctgct ggtgagctac
     8401 tcggataaac cgttggggct gttctgatat aagaagctgg tcgcattggg tattcattgc
     8461 ctgatgtgac gcttacgcgt cttatcatgc ctacgggaac ctgaccgtag gccggataag
     8521 gcgtacacgc cgcatccggc ataaacaaag cgcacgttgt tacaatttac gttttaaacc
     8581 tacgtgcgca ataatccaga ctacgccaac agaagtgcgt ttctggggcg tgttttgtca
     8641 ggagtttaat ctcgcgtttc gatggtgagc gggcgaacta ggccgtaaat caccattggt
     8701 gatccgaggc caatcagcaa taaac
//
LOCUS       DAJTSL010000164         8711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTSL010000164 DAJTSL010000000
VERSION     DAJTSL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.164"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            122..187
                     /gene="yobI"
                     /locus_tag="OO433_003446"
     CDS             122..187
                     /gene="yobI"
                     /locus_tag="OO433_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6051797.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            326..937
                     /gene="ruvA"
                     /locus_tag="OO433_003447"
     CDS             326..937
                     /gene="ruvA"
                     /locus_tag="OO433_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6051798.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            946..1956
                     /gene="ruvB"
                     /locus_tag="OO433_003448"
     CDS             946..1956
                     /gene="ruvB"
                     /locus_tag="OO433_003448"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6051799.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2006..2791)
                     /gene="znuB"
                     /locus_tag="OO433_003449"
     CDS             complement(2006..2791)
                     /gene="znuB"
                     /locus_tag="OO433_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6051800.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLALDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2788..3543)
                     /gene="znuC"
                     /locus_tag="OO433_003450"
     CDS             complement(2788..3543)
                     /gene="znuC"
                     /locus_tag="OO433_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6051801.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3622..4554
                     /gene="znuA"
                     /locus_tag="OO433_003451"
     CDS             3622..4554
                     /gene="znuA"
                     /locus_tag="OO433_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6051802.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLLGAKQV
                     TIAQLEDVKLLLMKSIHGDDDDHDHAEKSEEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4570..5892
                     /gene="mepM"
                     /locus_tag="OO433_003452"
     CDS             4570..5892
                     /gene="mepM"
                     /locus_tag="OO433_003452"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6051803.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6012..6983
                     /gene="lpxM"
                     /locus_tag="OO433_003453"
                     /gene_synonym="msbB"
     CDS             6012..6983
                     /gene="lpxM"
                     /locus_tag="OO433_003453"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6051804.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMVGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLQIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRRPGEIQPYKRKDLYPIK"
     gene            complement(7072..>8711)
                     /locus_tag="OO433_003454"
     CDS             complement(7072..>8711)
                     /locus_tag="OO433_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6051805.1"
                     /translation="DGLPHVDFSRVDKFFTYADAFRESLPIISGELYFEAHQGCFTSE
                     SATKAHNRNMENKLHDAEFFTTITNNMTSILRSEFDEIWKEMLTLQFHDILPGSCISR
                     VYLETEKEYIKLEAKTEKIISDTQSTLLSKIDTSSYKDPHILFNTTCFARNEWININN
                     NWLKARVNSYGYAVIDPKNKIVNGLKAESRSIENNYIKLLFSENGDLISLYDKRYGKE
                     YITENMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIYH
                     YNSSYLRFAFTLTQDSPLVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVIDR
                     PTHSDDSFAFAKDEIPAHHWVDLSDRETGIALIAKNKYGYRVKDQTLKLTLLRSQHKP
                     GEVVKTDNYDNFLINNFADIGKQKFIFSLFPHHGDYRVGGVINQAYIMNHPLQVVPVK
                     PHPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESHGLEISAMLSWVCNYHY
                     VQYVDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2373 a         2007 c         2126 g         2205 t
ORIGIN      
        1 tgtaaagacg atatctccaa tttcatattc agcagggtaa ttaatattca cgttgtcatt
       61 ccattgaaat agatacgaat atacgtaata gcataaaaaa attatttttt caggaggtgc
      121 aatgtatatt tttattacgc atttcttcac tgaatacgta atatttaaat atttgcttcc
      181 aatataacct gtagaataaa tcatactgcg ccatttttca gttcatcgag acacctcgca
      241 agttttcttc atccttcgct ggatatctat ccagcatttt tttatcatac agcattatct
      301 ttgattcatt acgcaggagc gtcatgtgat aggcagactc agaggcatca tcattgaaaa
      361 acaacccccg ctggtgttaa ttgaagtggg cggcgtaggc tatgaagtgc atatgccgat
      421 gacctgtttt tatgaactcc ctgaagcggg tcaggaagcg atcgttttca cccactttgt
      481 ggtgcgtgaa gacgcgcaac tgctgtacgg ttttaacaat aaacaagagc gcacattgtt
      541 caaagagttg atcaaaacca acggcgtcgg cccgaagttg gcgctggcga tcctctccgg
      601 aatgtcagcg cagcagttcg ttaatgccgt tgaacgtgaa gaagtggggg cactggtgaa
      661 actgccgggt attggcaaaa aaaccgccga acgcttgatt gttgaaatga aagaccgatt
      721 taaaggtttg catggcgatc tgtttacccc tgccgccgac ctggtactca catcacctgc
      781 cagcccggcg accgacgatg ctgaacagga agcggttgcc gcgctggtgg cgctgggcta
      841 taaaccacaa gaagcaagcc gcatggtgag caaaatcgct cgccctgacg ccagcagtga
      901 aacattaatt cgcgaagccc tacgcgccgc gttatgaggt aaaggatgat tgaagcagac
      961 cgtctgattt ctgccggtac cactttgccg gaagatgtag cagatcgcgc cattcgccct
     1021 aaattactgg aagagtatgt tggtcagccg caggttcgtt cacagatgga gattttcatc
     1081 aaagcagcga aactgcgcgg cgatgccctc gatcatttgt tgatttttgg tcctccgggg
     1141 ttgggtaaaa ctacgcttgc caacattgtc gccaatgaaa tgggcgttaa tttacgcacg
     1201 acttctggtc cggtgctgga aaaggcgggc gatctggcag cgatgctcac taaccttgaa
     1261 ccgcatgacg tgctgtttat tgatgagatc caccgtttat cgccagttgt tgaagaagtg
     1321 ctgtacccgg caatggaaga ctaccaactg gatatcatga ttggtgaagg tccggcggca
     1381 cgctccatta aaattgattt gccgccgttt accctgattg gcgcaaccac gcgcgcaggt
     1441 tcgctgacat caccgttgcg cgaccgtttt ggtattgtgc aacgtctgga gttttatcag
     1501 gtgccggatc tgcaatatat cgttagtcgc agtgcacgct ttatggggct tgagatgagt
     1561 gatgacggcg cgctggaagt tgctcgtcgc gctcgcggta cgccacgtat tgccaaccgt
     1621 ctgctgcgtc gagtgcgtga tttcgccgaa gtgaagcacg atggcaccat ctcggcagat
     1681 atcgctgctc aggcgctgga catgttgaat gtcgatgctg aaggtttcga ttatatggac
     1741 cgcaaattgt tgctggcggt aatcgataag ttctttggtg ggccggtagg tctggataac
     1801 ctggcggcgg ctattggcga agaacgtgaa accattgagg atgtgctgga accttatttg
     1861 attcagcaag gctttttgca gcgtacaccg cgtgggcgta tggcgacgac gcgggcgtgg
     1921 aatcactttg gcataacgcc gccagaaatg ccgtaagtcg gattggtcgc tcgcgccgca
     1981 tccgacaaat gtgttcagcg atagattagc tggcctgctt tttcatcata ctgataataa
     2041 ataacagtgc tgcacatagc accaccgacg gacccgccgg cgtatcgtaa aatgcggaaa
     2101 aggttaaacc gcccgttacg gcaaccatcc ccaccaacac agcgacacct gccatctgtt
     2161 ccggcgtgcg ggcaaagcga cgtgcagtag cagcaggaat aatcagcagt gaagtaataa
     2221 tcaacgcgcc aacaaatttc atcgctacac caatcgtcaa tgccgtcacc agcatcaaca
     2281 acaatttcac gcgctgtaat ttcacaccat caacaaacgc cagatccggg ctaatcgtca
     2341 tcgacagcaa attgcgccat tgccagaaca aaatagccac cacgatgacc acgccaatcg
     2401 caatagcgat gagatcttct ggcgtcactg ccagcaaatc accgaacagg taagccatca
     2461 aatcaacacg aatgttagac atcagactaa cgaccaccag gcccagcgac agggcactgt
     2521 gcgccataat ccctaataac gtgtcaagcg ccagctgtgg acgtttctcc agccatacca
     2581 gaccgcccgc caggagcagc gtaacggcaa ttaccgcata gaatggattc acgtccagca
     2641 acaaaccaaa cgcgacgcca agtaatgagg catgagccag cgtatcaccg aaataagaca
     2701 tacgacgcca gactacaaac gaacccagcg gacccgcggc acaggcgagc atgatcccgg
     2761 ctaaccaacc gggaaataat aattcaatca tgagcgatca tttccccgac gcaaaacaat
     2821 tcgtccctgt aaatcgtgac gatgattatg atgatggcga tagataccca gttgttcagc
     2881 accacgagga ccaaacatag aaataaactc cggatgtagg gaaacaactt ccggtgtgcc
     2941 ggaacaacaa atgtggtgat tcagacacag cacttcatcg gtttttgcca ttaccagatg
     3001 cagatcgtga gagaccatta aaacgccaca atccagttcg cgacgcaact ggtcaataag
     3061 gtcatataac gccacctggc catttacatc cacgccttga gtgggttcat ccagcactaa
     3121 taattgcggt cgatttaaca atgctcgcgc taacagtaca cgctgtgttt cgccaccaga
     3181 gagcttttgc atcggtgcgt taatcagatg cccggcctgg acacgtttca gtgcaggcaa
     3241 aatatcttct ttatgtgtgc cagggcgtaa gcgtaaaaaa cggtttacgg tcagtggcaa
     3301 cgtggtgtcg agatacagct tctgcggtac atagccgatg cgtagttttc cgttgcgctt
     3361 gataacccct tcatcgggtg ttaccagccc gagcactacc cgtaccagtg tcgacttacc
     3421 tgcgccattt ggcccaagta aagtcaaaat ttttccaggt ttaagttcca gcgacacatc
     3481 agagaggacg cggcgttggc caaaagaaac cgagacattt tccagggaaa ccagacttgt
     3541 catgttaatt ttagtcttgc agtagtcatg agatgttata atatcacact tctcatattc
     3601 attacgatga ttggtcgcat tatgttacat aaaaaaacgc ttcttttcgc agcattatcc
     3661 gccgctctct ggggaggtgc aacacaggcc gcagatgccg ccgttgtcgc ttcgcttaaa
     3721 cccgttgggt tcatcgcttc tgccattgct gatggtgtaa cagaaacgga ggttttactg
     3781 cctgacggcg cttcagaaca tgattattca ctgcgtccat cggatgtaaa acgcttacag
     3841 aacgcggact tagtcgtttg ggttggcccg gagatggaag cgtttatgca aaaaccggta
     3901 agcaaattac taggagcgaa gcaggtaacg attgcgcagc ttgaagatgt gaaactgcta
     3961 ctgatgaaaa gtattcacgg cgatgatgat gatcacgacc acgcggaaaa aagtgaagaa
     4021 gatcaccatc acggcgattt caacatgcat ctttggcttt ccccagagat agcgcgggct
     4081 acagcggttg caatccatgg aaaattagtg gaacttatgc cgcaaagtcg agccaaactt
     4141 gacgccaacc tgaaggattt tgaggcacaa ttagcctcaa ccgaaacgca ggttggtaac
     4201 gagctcgcgc cgctcaaggg gaaaggttat ttcgtttttc acgatgctta cggctatttc
     4261 gaaaaacagt tcggactgac accgcttggt cattttaccg ttaaccctga gattcaacct
     4321 ggcgcgcagc gtttacatga aataagaaca cagttggttg agcaaaaagc aacctgcgtt
     4381 tttgctgagc cacagttcag gccagcggtc gttgagagcg tcgcacgagg gacatccgtt
     4441 cgtatgggaa cgttggatcc cttggggacg aatatcaaac tgggtaaaac aagctattca
     4501 gaattcctga gtcaattagc caaccagtat gcgagctgcc tgaaaggaga ttaatgagga
     4561 agtgattacg tgcaacagat agcccgctct gtcgccctgg cgtttaataa tttaccgcga
     4621 ccacaccgcg ttatgttggg gtcgctcacc gttcttactc tggccgtcgc tgtctggcgg
     4681 ccctatgttt atcaccgcga tgccacgcca attgtcaaaa ccattgagct ggaacagaac
     4741 gaaattcgtt cgctcttacc tgaagccagt gagccgattg atcaagctgc acaagaagat
     4801 gaagccattc cccaggacga actggatgac aaaatcgccg gtgaagcggg cgtgcatgaa
     4861 tatgttgttt ccactggcga tacgctaagc agcattctca atcagtatgg tattgatatg
     4921 ggtgatatca cccaactggc tgcggccgac aaagaattgc gtaacctgaa aatcggtcaa
     4981 caactctcct ggacattaac cgcggacggc gaactgcagc gcctcacctg ggaagtgtct
     5041 cgtcgtgaaa cccgaaccta tgaccgtact gccgctaacg gttttaaaat gaccagcgaa
     5101 atgcagcaag gagagtgggt taacaatctg ctgaaaggta ccgtcggagg gagctttgtt
     5161 gccagcgcca gaaacgccgg tttaaccagc gccgaagtga gcgcagtgat taaagccatg
     5221 cagtggcaaa tggatttccg caaactgaaa aaaggcgatg aatttgcggt gttaatgtct
     5281 cgagagatgc ttgatggtaa acgtgagcaa agccagctgc tgggcgtacg tttgcgttca
     5341 gaaggtaaag attattacgc aatccgcgct gaggatggca aattctacga ccgtaacggt
     5401 actggtctgg cgaaaggatt cttgcgattc ccaacggcga aacagttccg tatctcgtct
     5461 aactttaacc cgcgtcgtac taatccggtg accggtcgcg ttgcaccaca cagaggtgtt
     5521 gatttcgcca tgccgcaggg tacgccagtg ctttcagtgg gtgacggtga agtggtggtt
     5581 gccaaacgca gtggcgcagc aggttattat gtggctattc gtcatggtcg cagctacacc
     5641 acgcgttata tgcacttgcg caagattctg gtgaaaccgg gacagaaggt gaaacgtggc
     5701 gaccgtatcg cgctttccgg taataccgga cgttcaaccg ggccgcatct gcactatgaa
     5761 gtatggataa accagcaggc cgtaaacccg ctgacggcaa aactgccgcg taccgaaggg
     5821 ctgaccggct ccgatcgtcg cgaattcctg gcgcaggcca aagagattgt gccgcagcta
     5881 cggtttgatt aattaacatc cattcgcagc cggtacgcag tcagtaccgg ctttttttat
     5941 ttggtgcggg gcaagttgcg ccgctacact atcaccagat tgatttttgc cttatccgaa
     6001 actggaaaag catggaaacg aaaaaaaata atagcgaata cattcctgag tttgataaat
     6061 cctttcgcca cccgcgctac tggggagcat ggctgggcgt agcagcgatg gtgggtatcg
     6121 ctttaacgcc gccaaagttc cgtgatccca ttctggcacg gctgggacgt tttgccggac
     6181 gactgggaaa aagctcacgc cgtcgtgcgt taatcaatct gtcgctctgc tttccagaac
     6241 gtagtgaagc tgaacgcgaa gcgattgttg atgagatgtt tgccaccgcg ccgcaagcga
     6301 tggcaatgat ggctgagttg gcaatacgcg gaccggagaa aattcagccg cgcgttgact
     6361 ggcaagggct gcagatcatc gaagagatga ggcgtaataa cgagaaagtt atctttctgg
     6421 tgccgcacgg ttgggccgtc gatattcctg ccatgctgat ggcctcgcaa gggcagaaaa
     6481 tggcagcgat gttccataat cagggcaacc cggtttttga ttatgtctgg aacacggtgc
     6541 gtcgtcgctt tggcggtcgt ctgcatgcga gaaatgacgg tattaaacca ttcatccagt
     6601 cggtacgtca ggggtactgg ggatattatt tacctgatca ggatcatggc ccagagcaca
     6661 gcgaatttgt ggatttcttt gccacctata aagcgacgtt gcccgcgatt ggtcgtttga
     6721 tgaaagtgtg ccgtgcgcgc gttgtaccgc tgtttccgat ttatgatggc aagacgcatc
     6781 gtctgacgat tcaggtgcgc ccaccgatgg atgatctgtt agaggcggat gatcatacga
     6841 ttgcgcggcg gatgaatgaa gaagtcgaga tttttgttgg tccgcgacca gaacaataca
     6901 cctggatact aaaattgctg aaaactcgca gaccgggcga aatccaaccg tataagcgta
     6961 aagatcttta tcctatcaaa taaaaaaaag cctctcaaat tattgagagg ctgtactcga
     7021 taaaaaatct caatgagtgt tcccactatt ttgagttttt aggttagtac gctattgagt
     7081 taatcgtagc gtgataattt caaaaggttt gaactcaaga ttgcaatatt gattgcaata
     7141 aaaactgtca tctaacttat tttcttgaag atcgacatat tgaacgtagt gataattaca
     7201 tacccacgaa agcatggctg aaatttctaa tccatgactc tcatataatc ttacgattat
     7261 accgtcgccg tcttcagcta attttattgt ttcgatgata acgctgttag tatcgatagc
     7321 aaaaaatgat agtgaagggg gtaattcacc cgggtgcggt ttgacaggga cgacctgtaa
     7381 gggatgattc attatatagg cttgattaat aacaccaccc acacggtaat ctccatgatg
     7441 aggaaataag gagaatatga atttttgttt accaatatct gcaaaattat taatcaggaa
     7501 attatcataa ttgtctgtct taacaacttc cccaggttta tgctgcgaac gcagcaaagt
     7561 taatttaagc gtctggtctt tgacacgata gccatatttg ttttttgcta ttaaagctat
     7621 gccagtttct cgatcagaaa ggtcgaccca gtgatgtgct ggtatttcat ctttggcaaa
     7681 agcgaacgaa tcatcgctat gcgttggtct gtcaattaca ccgaactgaa tttgacattg
     7741 tgcaagagat gtttgtactg acaccgggaa tttgactttg agagaaacat taggttcgtg
     7801 ccaatcaata aaagtttgga catcaacgag tggactgtcc tgtgtcaggg taaatgcgaa
     7861 gcgaagataa gaggaattgt agtgataaat cagcgtcatc gtagttttag gcccgctgat
     7921 tacagttgtc attttttcag cgagtaaaac atatgattcc ccatcacgat aatttgaagc
     7981 aaaatcccag gccgcgaaaa aacctgcatc ttcgtggtat gcacggatct cactgtgcat
     8041 attttcagta atgtattctt taccatatct tttatcatat agcgaaatta aatcaccgtt
     8101 ttctgaaaaa agaagtttaa tgtaattatt ttcaatactt cgagattctg ccttcagacc
     8161 atttacgatc ttattctttg gatcaatcac agcataacca taagagttta ctcgtgcttt
     8221 gagccagttg ttgtttatat tgatccattc gttacgagca aaacaggtgg tattaaataa
     8281 aatgtgcgga tctttataag aagaagtatc aattttactt aataaagtag actgcgtatc
     8341 agaaataatt ttctcggttt ttgcttctag tttaatatat tccttttcag tttcaagata
     8401 aactcttgaa atacatgatc cgggaaggat gtcatggaat tgcaaggtca gcatctcttt
     8461 ccatatttca tcaaactcac tgcgaagaat tgatgtcata ttattagtaa tagttgtaaa
     8521 aaattcagca tcatgtaatt tattttccat atttcgatta tgagctttag ttgctgactc
     8581 tgatgtaaag cacccttgat gagcctcgaa ataaagttca cctgaaataa tgggcaagct
     8641 ttcgcgaaaa gcgtcggcgt aagtgaagaa cttatcaaca cggctgaaat caacatgagg
     8701 taagccatca a
//
LOCUS       DAJTSL010000165         8650 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTSL010000165 DAJTSL010000000
VERSION     DAJTSL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.165"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..269)
                     /locus_tag="OO433_003455"
     CDS             complement(<1..269)
                     /locus_tag="OO433_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6051806.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVW"
     gene            complement(363..925)
                     /locus_tag="OO433_003456"
                     /pseudo
     CDS             complement(363..925)
                     /locus_tag="OO433_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1129..1335)
                     /locus_tag="OO433_003457"
     CDS             complement(1129..1335)
                     /locus_tag="OO433_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR6051807.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1442..2005)
                     /locus_tag="OO433_003458"
     CDS             complement(1442..2005)
                     /locus_tag="OO433_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6051808.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3380..5215)
                     /locus_tag="OO433_003459"
     CDS             complement(3380..5215)
                     /locus_tag="OO433_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051809.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5316..5603
                     /locus_tag="OO433_003460"
     CDS             5316..5603
                     /locus_tag="OO433_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6051810.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5575..7104
                     /locus_tag="OO433_003461"
     CDS             5575..7104
                     /locus_tag="OO433_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR6051811.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7274..8491)
                     /locus_tag="OO433_003462"
     CDS             complement(7274..8491)
                     /locus_tag="OO433_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6051812.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
BASE COUNT         2681 a         1955 c         1617 g         2397 t
ORIGIN      
        1 caccagacat cgttattaag tagcagcacc ataagataag gagtggcatc gttagccaca
       61 gcttccctac tccagaggta atataaaggg gtgggctcga ctgatttatc tttacgtcgc
      121 ttacactgca aatgttcaga aattagtcta tgcagctcac cagtaaaatc agcaatcaga
      181 gaaggaattg ccttattcat accagggcaa ggtattaatt taaactgtaa taatttaatt
      241 ttaggatgtg tagcagcagc ccgatacaga gttgcaagga cacacttttg ccagagggcg
      301 ttacaggaaa gcttaatgtt tgattctgta tacataataa atcaccttat agttacaata
      361 ggtcaaaaac cgctgtagcc aaagttacgc tggcctgatg ctttagtgtc gagcttcgtc
      421 aaataatcca gacgtaccaa taagcgctgg tactgctctg ggaaattagg atcgttaata
      481 tccaggatgt aacgtccatt agcagggaaa tgaactaatc cctggtgatc ttccagttcc
      541 aaccctaagg cactgtacca ggctccggta attaaccctc tcagggtgtc agagcgctca
      601 tagtttccca gatggtaata ggcgtctttg ttaaaaaaag gcagacgtga taatgacatt
      661 taaaggactg cgtatattct ttcgcccaga taatacaaac cgacgttgcg taggtgcgtt
      721 tgccttgttg ttttttacgg tgttgatctg cattaatttt cgcattcagc gagttacgga
      781 aacgagatat ctctccgggg atcaggttag ggaaacaggt aatgatatcc ccgttgtcaa
      841 tcacaggagg gtaatggaga tccccccgta ttgctgttat tctggaagtg tttttaatat
      901 tcgtattgat agttctcaga gatatatcac actctagata ttttaattaa gtatcagttc
      961 gatatcggca tattgtacac tacctcaggt agatgatgag atcagataca gggatcaact
     1021 gacggtgagc gcagtcccca cattttatcc agggcttact gcatattccc aacagccatt
     1081 cgttagccag gtagacgaat accctgattt gccgctggtt ttgctttcct atcgaagggc
     1141 ccatacatca tcacgcccac gaaacaaccg acgaaataac gcaactttct catccgtgga
     1201 taatacgaaa acgcactgca cagggctctg cggtttacgt cgccattcaa tcccatgagc
     1261 ttcaagtaat gagaccagcc gggtattttc tgcccgcagt actgctagtt catcgctgtc
     1321 agatttataa accatatgcg ttatcacttg tacctgtctc ccggcggcac cacgactgaa
     1381 attcgtgtga aggcaaatcc acgcgataac ttacgatagt tcggacataa gcccccataa
     1441 ttcagttatg tcgtggtcga ccccggttac ttttttcggg aagcctgcta cggcccagaa
     1501 taataccgtg ttctttctga tcctgtttag ccagatagct aagggcgtaa cgtaagtcgt
     1561 ctaccgcaga tttatcacta tagtgaatca cgtgatcgat acgcaccggg tatttgtctt
     1621 tagcccggca cagatgaaaa taaccctccc cttccgtgat ccgcctccag atatctccca
     1681 gttgcctgga tatctgataa gagttcttgt gacgttgtcc atccaggtag ccgataaaat
     1741 gaatatgaag ccctttgtct ggtgtatatt ccattaccca gtaatatccc gccaacatcg
     1801 tctgggtttc gctaagtaaa cggtatattt ccatacacat actgtgctta caggaatgcc
     1861 cgaaactggg cgtgtctttc ctgtaagcaa aatcaattct gaaaggtaac agtttagaaa
     1921 aacgttgaaa cataccatcc atgtgttcat tcacgtcttt cagaatcatg aaatccattt
     1981 tgtaattggg attggcatta tacattttat agtccttact ttataaaact cacaggaaga
     2041 gagaattacg taaatactga gagtagagaa acgccgtacc acggcattaa taaacgaagt
     2101 aaaccagcag cacagacaac ctgataaaac tgttttgtat taccgcatta ctgtgatgag
     2161 gtaatacttc agaatacagt gagactctct taaactgaga accagtacga attacaatgg
     2221 aaaaacacac ctcatagaat tgagtttcag gaagcaccac taaattcttt acaggaagta
     2281 atctgacatt cacaaaactc agtatcggct agctacaggc aacatcgcta ttaatattgt
     2341 cgaaccatta agtaaagccg agagagacca cgggtattta atcattagca taaaagaata
     2401 atggtataca gaacataccc acacaccata agaagattgt tccatacaga aaagatcata
     2461 tttacctata cctataaact gtaacggagt cataacacgc tctaatagtg ttccgatacg
     2521 taaccagtgt taagtacgta caggacaaca ttacacacat aattcaccat acagcttccc
     2581 ccatatgctt cactgcgtag tgtatggaga tatatgcatt acacacatac agaatactta
     2641 gcataaagag gagtgacatt accacgtaaa gagataagtt atacaacaaa catacaaaca
     2701 atacggcaca gacacacaat accataactc caactaataa tactaattaa ccttaattat
     2761 ttatggtata tgaaataaat gtgattctcc cggaactaac taacctaacc agacagatga
     2821 tcactgtata aaaacttcag ttaatactca tttgttttgt cgactcagta tataatgagt
     2881 gaccagtaat caggataatc acacttacat aacctgtaaa aaagaacaag aagaataagc
     2941 cactaacata atgcaaaatg gcatctgaat cgatcgcagt ttggcattcg aatcgatcat
     3001 gatttggccc aatccgtgat cgaatagagc ggaatacgca acataattca ccagacaaga
     3061 gcttacaccg aattacagat tatgtattaa tgtcaaaact ggcatatttt acagatacaa
     3121 tgaacatgca acccatcaat ctaatagggg cggtatgtac atctgcccat gaatgggatg
     3181 gcgctaagaa tacaaaacaa ttagacatag atcacaaaaa cacaccattc taattgaaaa
     3241 acaaaacaaa caaataaatc atactattat agaattttct cttgccagac atagataata
     3301 cccctgctaa ttttgctaat ggttagcaaa attagcaggg tatataaaga ttattgaccc
     3361 aaaacacact cagatgaatt cacgacagct caggagggtt attcctgatg tcatcttcac
     3421 aaataatggt taacgcatca tatatcattc tgcgtctgtt cctgttttcg gccttatcgc
     3481 catacaacat catcacattt ttaatttcat cgaccggtga tttcacatag tgaactaata
     3541 caccaagctt ctcaacacat tgcagtaaag tatatttctt actgcctgtg aaaagcccta
     3601 tcatagtttc tttacgttcg cgactggata atactttgtt ttccataatg acttctgcca
     3661 gcgtcttacc atcataaaca tactctttat ctatcagaat atttatctta tccagcacct
     3721 tctctacgtc atcaagcaaa cctgtaatat atgggggcgc aatatatggt ttcgtactga
     3781 tcttttcaag catacaatta tacatcttaa taacacgcat cagggtaaaa ttgttcgcat
     3841 catactgtat ttcaccctcc ttatatacag gatcaataag tggtattttt tcaataaatg
     3901 catcctgata ggcttttaca tggtcaagcc aataaaacaa cgagccatac tttatatttt
     3961 ttctatttac tttaacctcc agtgctgtcc gaagcaacga taatgccccc ctgacatatc
     4021 ctgaaggtaa ttcaccaaac aatccttttc tttccatgtc atcataatgc tgcagcacat
     4081 cattaatttt atttttcctt atcagacaaa tgtacaccgc aagtgtttcg tagcagctgg
     4141 tcacatgaac agcctcattc tgcaactgcc caaaatagct tataatcttt ttcagatatg
     4201 cgtcatcaca accattctta tcattaatat taaatacaag atctttcaga cgggaaatat
     4261 attcctgaga tttatcatca agtgaaacac tataacgaga caatgttcct tctgcactgt
     4321 tttgaaactt ataaaacatt tccagtaatt tttttgcata aggcgttgta tcaaagctgg
     4381 taaagtcata aagatattgc gcagcgtaac catacaaacc atgaatccag agtccttctc
     4441 tcaccaacac tctgagataa tatgcaaggc tttgaagtat cttcgtcttt cgttgtatat
     4501 caagtaagac acttaatgaa taaaatgcat tatagaattt atcaatattc ctgagcactt
     4561 tctccagttc atccttatcc agcttcttct cccggtaatc ctttttatat tcatttttga
     4621 tagccatgat atattgttgc atcaggctct tgcttttccc gttatccttc cgtatagttc
     4681 ctgtatattt ccccagagca tacagctttc cattttcatg aaaatcttcc ttgttgaatc
     4741 ccagttcagc cagatattcc cgaagataac gtttaacagc agcttcatcg ctttctgtat
     4801 cagcgtgatc aagacaagga caatacgatt tctgtaaaaa attataaata tgataataaa
     4861 aagcagagtc attctctggt ctgtatgtat tatacatatc tacaacaaga aaaataaccc
     4921 caaaagtgcg cagacaatag ctgcgaagga attcaggttt tagtgtggaa tgcactggat
     4981 aagaattcat catgtaataa aaatgttcat atcgggcata tatgatccgc cgttgctcct
     5041 gactgaacac cccttcagca tcaataatac gactgatctc attataacgt tgatcccaga
     5101 atttttgatg catttcaaaa ttggtactct tccgcttgtg tgccggattc tcgccttcct
     5161 taaacaaccc caaaacagta caaatatcat acaacatatt tgtaaatgtg cgcatatgct
     5221 gtgctctccg gttggtttgc attttatcgc agaggacatt ctgagcttaa cgtatcaaac
     5281 tgacacaacg acatgcaatg catatggaga acaatatgtc aaatcaaacc ggacagcttt
     5341 acacgccttc acgcatcctg cgtatggctg aacttacccg actgctgggt atatcaagat
     5401 ctactattta cgtgaaaata aatccggcct cgaaatacca cgaccctacc tttcccaaac
     5461 caataaggct tggtagtgca tccgtaggct ggcgggcatc tgccatcgat gagtggctga
     5521 tgttacatac ggcaccagcc tggtctgaac ctgaaaacaa actaaaggaa cttaatgatg
     5581 acgacaaaac tatcactccc tgaacttgac acaactaaac tgcctgacag gtgccaggca
     5641 ttacttgatg aaatgcatga agaaacagga atacgccgtg aaatacttct gtctgttatg
     5701 ctgacggtca tggctgcctc ggtccaggat acgcatgaag ttgaactttc cggagggcaa
     5761 cgtaccagcc ttcagatatt tatgtgtctt tcatcagcat caggcagtgg aaaaacctct
     5821 gcctgcgcaa aattaatcgc tcctgttcat gaaacagaag aagaactaca tcaggcatat
     5881 atcgacgata aaaaaaacta tgaccgaatg atggaaatgt ggacaacaga taaaaaaatc
     5941 ctggagcgga gatataaaaa ggaaattgaa agatccccgg aaaatgctgc tgctgcacga
     6001 gcagcacttg aaaagtgtat agcaaataaa cccgaacctc ccgtacaaca ggttctcatc
     6061 gtgaatgacg cgacaccaga agggatagct ttgaaactca gccagtcacc ctctcttctg
     6121 ttgctgtctg atgaaggagg tactattctg gataagcgtt tcgaacgcaa atcggcactg
     6181 tacaacacat tatggagtgg gcaaccagtc aacgttgaac gggcatccag acccgggttc
     6241 cggctgaatg atgtccgtct cactatactt atactgactc aaccggtaat atttaataag
     6301 ttttttactc tcactggcga tcagatccgg ggcaatggct ttctggcccg gttgctattt
     6361 tgcgaacccg gagcaattaa aataatgacg acagaaccgc atactgccac tcctgttgtg
     6421 acgcaacaat gtgcaagctg ttttcgtaag aaaagttttg gcagtcagat cagagactct
     6481 ctgagagcat ccagagaaag acgtgcaaaa agtaaagagc gcatctgcat gacattatca
     6541 aatgcagcat cccgcgcact ggagcgcttt cacaaagaaa atatggatac tgtcagacag
     6601 aatcctcata tgggtacatt tgaagacatt attgtcagaa aaagagagca ggttgtcagg
     6661 atagcagcac ttctggaact ggagaaagat ccagactcga cagtaataac gctggaaagt
     6721 actaatagcg ccatttatct cattgaattt tatttcaagc atcttatata taaacttgaa
     6781 tcccttcggg aaatatcccc ggctgagaaa cttgataaat ggcttcagaa aagaattatc
     6841 acaacagcag gttacatatt ccagaaaagt tatattttac aatatgcccc atatgctctc
     6901 aggaaaaaat gtgttctgga tgaagcactt gacatactgg cggaacaaag gaaaataagg
     6961 attgatgata acctggttgt ttatatagga aacacgatta caccatccga actggcaaaa
     7021 aaactaaata ttcctgcatt cgatgccgga gttttcattt gtgatcatca aaacatactg
     7081 aagtatcacc gaaacagact atagatacta tcggacaaaa aataaattat cacaagtgta
     7141 ttattcgtta taataatgaa taatcgttat ctgagtgcaa tgccaggttt tcattacgaa
     7201 ctggcattgc atttattttt caaaaaagaa agcagtaacc atttggctac tctgagtatg
     7261 ccagtccatg aaactaagcg aattccttag tgtttttagc aagttgagac atcacataat
     7321 ccgcccatgc ctgcatcatc ggtacacgct gctccagtag atcagtacga tgatatgccg
     7381 cctcaacctt atttttcagc gtatgagcga gcgccctttc cgccagatcc cgcgaatacc
     7441 cctgttcact acaccagtcc ctgaatgttg agcgaaaacc atgtgccgtg gcaactcgcc
     7501 ccggaatgtc actgacggct ttctttttac gcagaaaact tgtcaacacc atatcggaaa
     7561 ggatctgctg ctttctgggt gaagggaaca ccagttcatc atgcaggcca cgtatatttt
     7621 ccagaatgta aatagcctgc cgggataaag gaacacgatg ctgtagccta gctttcattc
     7681 tttctgcagg tatagtccat acccgcttat gaaaatcaat ttcagcccag cgcattcccc
     7741 tggcttcgcc cgagcgagtt gctgtaagta tcaccattaa taacagtgcg cgggtaacat
     7801 tataaggttc atcactatac acactggtcg ccacaaaaag cggtaactgc ctccagggca
     7861 ttgcgggttg gtgttcatca cgtcctcttg tctgctgagg aagcaaatgg tcaaccacat
     7921 caacaggatt tgccacacaa aaaccgtgcg cccatcccca ctgcataaca acatgaatgc
     7981 gctgtttaac ccggcttgcc gtttctgaca aggttaacca gactggacgc agtgtttctg
     8041 ccacgtccgc agccgtaatc gaatccagcg tttttgctcc cagttgagga aacgcgtaat
     8101 tctcaagcgt cgataaccac tgccttacat gctttggatt ctcccatcca ggagacagtt
     8161 ctgcatgtac acgcctggct gcatcggcaa atgttgggat aacgactttc tcagattcag
     8221 cctttttaat ctccagagga tcatcacctg cagcaagttg ctctcgcatt atccgggcag
     8281 tacgtgcagc ttcagcaata ctgacctctg ggtaagttcc caatccagca ttacgtcttt
     8341 tttgtgtcac cggacttaca taacgaaaaa cccatttccc ccgccccttt actgaagaag
     8401 gatgaagggt cagtccggta attcccccat ggggcaatgg tttgtcatca ggtttgatat
     8461 gtcttgcttt cgtatccgtc aatactgcca tacgctaatt cccgtcactc tggtatgcca
     8521 tccagtatgc cattagtccc tcgcttcact aagaatacat tagataacat cggacaacaa
     8581 aaagtgtaat acattgttta taaagaaatt atataacacc cctggattaa gtcagattta
     8641 tttcaggcgg
//
LOCUS       DAJTSL010000166         8626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTSL010000166 DAJTSL010000000
VERSION     DAJTSL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.166"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..66
                     /locus_tag="OO433_003463"
     CDS             <1..66
                     /locus_tag="OO433_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR6051813.1"
                     /translation="RYQAKYVSAIWQKLIPSQADF"
     gene            125..328
                     /locus_tag="OO433_003464"
     CDS             125..328
                     /locus_tag="OO433_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCR6051814.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENELPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            328..1353
                     /locus_tag="OO433_003465"
                     /pseudo
     CDS             328..1353
                     /locus_tag="OO433_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1381..1470)
                     /locus_tag="OO433_003466"
     tRNA            complement(1381..1470)
                     /locus_tag="OO433_003466"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1434..1436),aa:Ser,seq:cga)
     gene            1564..2361
                     /gene="mtfA"
                     /locus_tag="OO433_003467"
     CDS             1564..2361
                     /gene="mtfA"
                     /locus_tag="OO433_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6051815.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2462..2537
                     /locus_tag="OO433_003468"
     tRNA            2462..2537
                     /locus_tag="OO433_003468"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2495..2497,aa:Asn,seq:gtt)
     gene            2851..>8626
                     /locus_tag="OO433_003469"
     CDS             2851..>8626
                     /locus_tag="OO433_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6051816.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYILGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFNNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAESWLPSWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     VGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNNAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVMVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYR
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGVAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLKALNTNENGIAEVTLKGTVLGVYTAEAILLNGKSDTKIVNIVPDTSNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGMHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTKPADTQLSQSTSNTNDSGVAEVTLKGTVLGVHT
                     VEATLPNGNGYTTTVNIAPDTSNAQVTLNIPAQQVVTNNSDSVQLTAMVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNGVNNLPVTFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEKTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGETTSLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQLTATLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLKGTKAGAHTVTAS
                     MTGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDIN"
BASE COUNT         2468 a         2068 c         2103 g         1987 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagctatc tggcaaaaac tgatcccgag tcaggctgat
       61 ttttaatgtt caaccctaat tgccgctaac cgtatatagt tagcggcggt tatgagatat
      121 agctatgagc agcttatttt taaccgaaga tgaattgcta atattaacgg gctgcaaata
      181 tgcaagccac cagcgaaaat ggttaatgga aaacgagctt ccgttctata ccaatcgtag
      241 tggcaaaccg attgtcagcc gggatctatt tacctgcaat aaaactttac caccacgcga
      301 ggtagagccg aattttggtg cgatctgatg ggaagacgaa ggaaaaatcc tgagcacgaa
      361 aaattacctc caaatgtata cccaaataaa tatagttatg tatggaaacc aacatccagc
      421 gaatctgtaa cactaaccgc catcaaggat ggtttagctg ctttatggaa aaagtatgag
      481 gaaactgtaa ataatcgcga tcgtgcaatg acattcggtc gcttgtggga aaaattcctc
      541 gccagcgcct attacagtga cctcagtcca agaacacaaa aagattatct gcaacatcaa
      601 aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca gaacacatcc
      661 gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat gaagtcccgc
      721 gtttacagtt gggggtatga gcgagggtac gtgaaggcta acccatgtgc aggtgtaagt
      781 aaattcaagg ccaaaaaccg cgaacgatat gtaaccgaca aagaatacca ggcagtatta
      841 agcgttgcac ctcttcctgt ttttatcgca atggaaattg cctatctgtg tgcagcgagg
      901 gtttccgatg tgttatcgct gaaatgggag cagattggaa acgacgggat ctttatccag
      961 caagggaaaa caggaaaaaa acagataaaa gcatggagtc cacgattaca ggcggcgatc
     1021 gaaaaagcaa aacagttacc aacatccgcc tatgtaatca gcaatcaata cggcaaccga
     1081 tatatgtaca aaggctttaa cgaaatgtgg gtagaagcaa gaaatcgcgc aggcaaaatt
     1141 tcaggtattt taaccgactt cacctttcat gatctgaagg cgaaaggaat ttcagactat
     1201 gaaggaagca gtcgggataa gcaacttttc tctggtcaca aaaccgaggg gcaagtgcta
     1261 atctatgaca ggaaggttaa agtttcaccg acacttgatg tcccgttacc tgaaaatatt
     1321 ccaagtaatt ccacgtgtga tttttgtcac tgacttaatg atgtataagt gattgaattt
     1381 tggcggagag agggggattt gaacccccgg tggagttgcc cccactccgg ttttcgagac
     1441 cggtccgttc agccgctccg gcatctctcc gttcagatgg ttgccatgat gccaggaaat
     1501 ttggcatttt aacagtccct gtccgtgcaa ttttgttcaa gtgacgagtt tgcgagcaaa
     1561 acgatgatta agtggccctg gaaagtacaa gaatcagcac atcaaactgc ccttccctgg
     1621 caggaagcac tatcgatccc ccttttaacg tgtctgacgg aacaggaaca aagcaaatta
     1681 gtcactcttg ccgaacgttt tttacagcaa aagcggcttg ttcctttaca gggctttgaa
     1741 ctggattcat taagaagctg ccggatagca cttctatttt gcctacccgt tctggagtta
     1801 ggactggaat ggctggatgg ttttcatgaa gtcttaattt atcctgcgcc atttgtggtc
     1861 gatgatgaat gggaagacga tatcggtctg gtgcataacc aacgtattgt tcagtcaggt
     1921 cagagctggc agcaagggcc tatcgttttg aactggttgg atatacaaga ttcttttgat
     1981 gcttctggtt ttaacctgat tattcatgaa gttgctcata agctggacac ccgtaacggc
     2041 gatcgcgcca gcggagttcc ctttattccg ttgcgtgagg ttgctggctg ggaacacgat
     2101 cttcatgctg caatgaacaa cattcaggaa gaaatcgaat tggttggcga gaatgcggcg
     2161 agcattgatg cttatgcagc cagcgatcct gctgaatgtt ttgccgtact ttctgaatat
     2221 ttctttagcg ccccagaact ttttgctcct cgtttccctt cattgtggca acgtttctgc
     2281 caattttatc aacaagatcc tttgcagaga ctgcatcacg ctaatgatac agactcgttt
     2341 tcggcgacga atgttcatta attaacaact ttgcatatta attaatcaat tgaaatgact
     2401 tatgaaattt agtgttgaca gacaaggtac cgctaagtaa tatgcgcccc gttcacacga
     2461 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     2521 tccagtcaga ggagccaaat tctaaaaatt cgctttttta gcgcaatgtc actgacctta
     2581 gttgaacatt gttttttaac ggatagcggg tttttaacat cttaagcgcc ctcgaccttt
     2641 atggttgagg gcgttttgct atgaacgcca tcaccatttt cccctcgatt ataaaacttg
     2701 agttattcag tagtctcccc tcttgcaact cacacccaaa actgcctaac gaaaagttat
     2761 taattttcaa tcatattgct atcagtattt acattttttc gctgtgctag aaagggcgca
     2821 tttatgttag ctcgttcagg gaaggtaagc atggctacga agaagagaag tggagaagaa
     2881 ataaatgacc gacaaatatt atgcgggatg ggaattaaac tacgccgctt aactgcgggt
     2941 atctgtctga taactcaact tgcgttccct atggctgcgg cagcacaagg tgtggtaaac
     3001 gccgcaaccc aacaaccagt tcctgcacaa attgccattg caaatgccaa tacggtgccc
     3061 tacatccttg gagcgctgga atcggcccaa agcgttgccg aacgtttcgg tatttcggtg
     3121 gctgagttac gcaaactcaa ccagtttcgt acgtttgctc gaggttttaa taatgtccgc
     3181 cagggtgatg aactggatgt cccggcacaa gttagtgaaa aaaaattaac cccgccgccg
     3241 ggtaatagca gtgacaacct cgagcaacag atagccagta cttcacagca aatcgggtct
     3301 ctgctcgccg aagatatgaa cagcgagcaa gcggcaaata tggcgcgtgg atgggcctct
     3361 tctcaggctt caggcgcaat gacagactgg ttaagccgct tcggtaccgc aagaatcacg
     3421 ctgggcgtgg atgaagattt tagcctgaag aactcccagt tcgattttct ccatccgtgg
     3481 tatgaaacgc ctgataatct ctttttcagt cagcatactc tccatcgtac tgacgagcgt
     3541 acgcagatta acaacggctt aggttggcgt catttcactc ccacatggat gtcaggcatc
     3601 aacttctttt tcgaccacga tcttagccgt taccactccc gcgccggcat tggcgcggag
     3661 tactggcgcg actatctaaa attaagcagt aacggctatt tgcgactgac caactggcgc
     3721 agcgcacctg aactggacaa cgattatgaa gcacgcccgg ccaatggctg ggatgtacgc
     3781 gcagaaagct ggctaccctc ctggccgcac cttggcggta aactggtcta tgaacagtat
     3841 tatggcgatg aagtggccct gttcgataaa gacgatcggc aaagtaatcc tcatgccata
     3901 accgctggac ttaactatac ccccttcccg ctgatgacct tcagcgcgga gcaacgccag
     3961 ggtaaacagg gcgaaaatga cacccgtttt gccgtcgatt ttacctggca acctggcagc
     4021 gcaatgcaga aacagcttga cccgaatgaa gtcgctgcac ggcgtagcct tgcaggcagc
     4081 cgttatgatc tggtggatcg caacaacaat atcgttctgg aatatcgcaa aaaagaactg
     4141 gttcgcctga ccctgacaga ccccgtgaca gggaagtcag gagaagtgaa atcactggtt
     4201 tcgtcgctac aaaccaaata tgccctgaaa ggctataacg tcgaagccac cgcactggaa
     4261 gctgccggtg gcaaagtggt cacaacgggt aaagatattc tggttaccct gccggcttac
     4321 cggttcacca gtacgccaga aaccgataac acctggccga ttgaagtcac cgccgaagat
     4381 gtcaaaggca atttgtcgaa tcgtgaacag agcatggtgg tcgttcaggc acctacgcta
     4441 agccagaaag attcctcggt atcgttaagt acccaaacat tgaacgcgga ttcccattca
     4501 accgccacac tgacttttat tgcgcatgat gcagcaggta atcctgttgt cgggctggtg
     4561 ctctcgacgc gtcacgaagg tgttcaggac atcacccttt ctgactggaa agataatggt
     4621 gacggaagct atacccagat cctgaccaca ggagcgatgt ctggcacgct gacgctgatg
     4681 ccacagctga atggtgtgga tgcggctaaa gcccccgccg tggtgaatat catttctgtt
     4741 tcgtcatccc gaactcactc gtcaattaag attgataagg accgttatct ctccggcaat
     4801 cctatcgagg tgacggtaga actgagagat gaaaatgaca aacctgttaa ggaacaaaaa
     4861 cagcaactga ataacgcagt cagcatcgac aacgtgaaac caggagtcac tacagactgg
     4921 aaagaaaccg cagatggcgt ctataaggcg acctataccg cctataccaa aggcagtgga
     4981 cttactgcga agctattaat gcaaaactgg aatgaagatt tgcataccgc tggatttatc
     5041 atcgacgcca acccgcagtc agcgaaaatt gcgacattat ctgccagcaa taatggtgtg
     5101 ctcgccaatg agaatgcagc aaacaccgtc tcggtcaatg tcgctgatga aggaagcaac
     5161 ccaatcaatg atcataccgt cacgtttgcg gtattaagcg gatcggcaac ttccttcaac
     5221 aatcaaaaca ccgcaaaaac ggatgttaat ggtctggcga cttttgatct gaaaagtagt
     5281 aagcaggaag acaacacggt tgaagtcacc cttgaaaatg gcgtgaaaca aacgttaatc
     5341 gtcagttttg tcggcgactc gagtaccgcg caggttgatc tgcagaagtc gaaaaatgaa
     5401 gtggttgctg acggcaatga cagcgccaca atgaccgcga ccgtccggga tgcaaaaggc
     5461 aacctgctca atgacgtcat ggtcactttc aatgttaatt cagcagaggc gaaactgagc
     5521 caaaccgaag tgaatagcca cgacgggatc gccacagcta cgctgaccag tttgaaaaat
     5581 ggtgattata gggttacggc ctctgtgagc tctggttccc aggctaatca acaggtgaat
     5641 tttatcggtg atcaaagtac tgctgccctg accctcagtg tgccttcagg tgatatcacc
     5701 gtcaccaaca cagctccgca acatatgact gcaaccttgc aggataaaaa tggcaaccca
     5761 ctaaaagata aagaaatcac cttctctgta ccaaacgacg tcgcaagtcg gttctcgatt
     5821 agcaacagcg gaaaaggcat gacggatagc aacggggttg caatcgcctc cctgaccggc
     5881 acgttagcgg gcacgcatat gatcacggct cgtctggcta acagcaatgt cagcgataca
     5941 cagccaatga cgtttgtggc ggataaagac agagcggttg tcgttctgca aacatcgaaa
     6001 gcggaaatca ttgggaatgg cgtggatgag acaactctga cagcaacagt gaaagatcct
     6061 tttgataacg tggttaaaaa tctgtcagta gtcttccgca cctcccccgc agacacgcaa
     6121 ctgagtctga aggcgcttaa tactaatgag aacggtattg ctgaagttac ccttaagggc
     6181 acggttttgg gtgtttatac agccgaagcc atactgctta acggtaaaag cgatacgaaa
     6241 atcgtcaata ttgtgcccga taccagcaac gcgcaggtca ccctgaacat cccagctcaa
     6301 caggtggtga cgaataacag cgacagcgtg cagctgacgg cgacggtgaa agatccgtcg
     6361 aatcatccgg tggcgggaat aacggtgaac ttcaccatgc ctcaggacgt ggcggcaaac
     6421 tttacccttg aaaataacgg tattgccatc actcaggcca atggcgaagc gcatgtcacc
     6481 ctcaaaggta aaaaagcggg catgcatacg gttaccgcaa cgttgggtaa caataatgcc
     6541 agcgatgcgc aacctgtcac attcgtggcg gataaggaca gcgcagttgt cgttctgcaa
     6601 acatcgaaag cggaaatcat tgggaatggc gtggatgaga cgactctaac agcaacagtg
     6661 aaagatcctt ttgataacgc agtaaaagat ctacaggtta ccttcagtac caagccggca
     6721 gatactcaac ttagtcagag cacaagcaat actaacgaca gtggtgttgc tgaagttacc
     6781 cttaagggca cggttttggg cgttcataca gtcgaagcca cacttcctaa cggcaacggc
     6841 tatacaacga cagtcaatat tgcgcccgat accagcaacg cgcaggttac gctgaacatc
     6901 cctgctcaac aggtggtgac gaataacagt gacagcgtgc agcttacggc gatggtgaaa
     6961 gatccgtcga atcatccggt ggcggggata acggtcaact tcaccatgcc acaggacgtt
     7021 gcggcaaact ttaccctcga aaataacggc attgccatca cccaagccaa tggggaagcg
     7081 catgtcacgc tgaaaggtaa aaaagcgggt acgcatacgg ttaccgcaac gctgggtaat
     7141 aacaatacca gtgattcgca gccggtaacg tttgtggcgg acaaaacctc ggctcaggtt
     7201 gtcctgcaga tgtcaaaaga tgagatcaca ggtaatggcg tcgataacgc aacgctaact
     7261 gcaacggtta aagatcagtt cgacaatggg gtgaataatc ttccggtaac attcagctca
     7321 gcctcttcag gactcaccct gaccccggga gtaagtaata ccaatgagtc tggcatcgcg
     7381 caggccactc tcgcaggcgt tgcctttggt gagaagacgg ttactgcatc actggctaat
     7441 aatggtgcca gcgacaacaa aactgtgcat tttattggcg acacagcggc ggcaaaaatt
     7501 atcgagttga cgcctgtccc agacagcata atcgccggta ccccgcagaa cagctccggc
     7561 agcgtcatca ccgccacagt cgttgataat aatggctttc cggtgaaagg tgtgactgtg
     7621 aacttcacca gcaacgcagc gacagccgaa atgacgaacg gcggtcaagc cgtgacgaac
     7681 gaacagggta aggctaccgt cacttatacc aatacccgct cctcgataga atcaggagcg
     7741 agaccggata ccgttgaggc cagtctggaa aatggtagct ccacgcttag cacatcaatt
     7801 aatgtcaacg ctgatgcgtc tacggcacat ctcaccttgc tacaggcact ttttgataca
     7861 gtctccgcag gcgagacaac cagtctgtat attgaggtga aggataatta cggcaacggt
     7921 gtcccccagc aggaggtaac cctcagcgtt tcaccaagtg aaggcgtgac ccccagtaat
     7981 aacgctatat atactaccaa ccacgacggc aatttttacg caagctttac cgctacaaaa
     8041 gccggggttt atcaattgac ggcaaccctc gaaaatggcg attcgatgca acaaacagtg
     8101 acctatgtgc cgaacgtcgc gaatgctgaa atcacgctgg cagcctcgaa ggatccggtg
     8161 attgccgaca ataacgatct cacgacacta acagcaacag tcgctgatac agagggcaat
     8221 gcgatagcca acactgaggt aacatttact ctgccggaag atgtgaaggc gaacttcacg
     8281 ctgagcgatg gcggtaaagc gattactgat gctgaaggca aagcgaaagt cacgctgaaa
     8341 ggtacaaaag caggcgctca tactgttaca gcatcgatga ctggcggtaa gagtgagcag
     8401 ttggtggtga actttattgc ggatacgctc actgcgcagg ttaatcttaa cgttaccgag
     8461 gacaatttta tcgctaataa cgtcgggatg accaggctgc aggcaacagt gactgatgga
     8521 aacggcaacc cgttagccaa tgaggcggtg acattcacgc taccggcaga tgtgagcgca
     8581 agctttactc tcggacaagg cggttccgcc attactgata tcaacg
//
LOCUS       DAJTSL010000167         8621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTSL010000167 DAJTSL010000000
VERSION     DAJTSL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.167"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..190
                     /locus_tag="OO433_003470"
     CDS             <1..190
                     /locus_tag="OO433_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051817.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHAL
                     FPIFTASWRAYRIMNKGA"
     gene            413..910
                     /gene="ubiC"
                     /locus_tag="OO433_003471"
     CDS             413..910
                     /gene="ubiC"
                     /locus_tag="OO433_003471"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6051818.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            923..1795
                     /gene="ubiA"
                     /locus_tag="OO433_003472"
     CDS             923..1795
                     /gene="ubiA"
                     /locus_tag="OO433_003472"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6051819.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO433_003473"
     CDS             complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO433_003473"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6051820.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4544..4912
                     /gene="dgkA"
                     /locus_tag="OO433_003474"
     CDS             4544..4912
                     /gene="dgkA"
                     /locus_tag="OO433_003474"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6051821.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5022..5630
                     /gene="lexA"
                     /locus_tag="OO433_003475"
     CDS             5022..5630
                     /gene="lexA"
                     /locus_tag="OO433_003475"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6051822.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5703..7028
                     /gene="dinF"
                     /locus_tag="OO433_003476"
     CDS             5703..7028
                     /gene="dinF"
                     /locus_tag="OO433_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6051823.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7144..7353
                     /locus_tag="OO433_003477"
     CDS             7144..7353
                     /locus_tag="OO433_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6051824.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO433_003478"
     CDS             complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO433_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6051825.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQSTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8228..8621
                     /locus_tag="OO433_003479"
                     /pseudo
     CDS             8228..8621
                     /locus_tag="OO433_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
BASE COUNT         2033 a         2046 c         2310 g         2232 t
ORIGIN      
        1 tgatatgttg aatcctacaa aagataccaa ttggaattct acgtgtatct ataaatccag
       61 acataagatg ttgcctgtta atcttactca ggaaacactt ttcaactcca aatctcagga
      121 taaacatgcg cttttcccca tttttactgc gagttggcga gcttatcgta taatgaataa
      181 gggtgcttaa gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat
      241 ggttgccctc gccagcgcgg gcatcggcaa agcgtaaggt tcaaaagcga tttaccactt
      301 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      361 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      421 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      481 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      541 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      601 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      661 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      721 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      781 gacattaacc cgggacttta ttgagatagg ccgtgatgcc gggctgtggg ggcgacgttc
      841 ccgcctgcga ttaagcggta aaccgctgtt gttaacagaa ctgtttttac cggcgtcacc
      901 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
      961 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1021 atgggcgttg tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtatttgt
     1081 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgaaa
     1141 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1201 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1261 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1321 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1381 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1441 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1501 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1561 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     1621 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1681 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     1741 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1801 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     1861 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaatgcc ggataaggcg
     1921 ttttcgccgc atccggcaat tctctctggt tacccttcgc cctgcgtcgc actctcaatc
     1981 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2041 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga
     2101 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga
     2161 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttagcg
     2221 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
     2281 agctgtagcg tgcgagaatg cgacgggttg atatgcaact catcatcttg cagggtaatc
     2341 agcccctgac gttgcatctc atttgccaac gcatcaataa cgtccggcaa ctcgtcgcga
     2401 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2461 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2521 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2581 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2641 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     2701 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtaagtgag
     2761 cgctgacgtg atgccagtag cgcggtacag cagaggttca tggcgtttgc cgcgcctgcg
     2821 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2881 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
     2941 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3001 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
     3061 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3121 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3181 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
     3241 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3301 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3361 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3421 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3481 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3541 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     3601 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3661 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3721 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     3781 aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag ttttatccgt gccgtgttca
     3841 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3901 cacagtacgg cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     3961 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4021 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4081 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4141 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4201 gcgcgtaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4261 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
     4321 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4381 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4441 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4501 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4561 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4621 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4681 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     4741 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4801 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat
     4861 tatcgtcgcc gtgataacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4921 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     4981 atatactcac agtataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
     5041 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5101 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5161 tgaaggcgct ggcacgcaaa ggagttattg aaattgtttc cggcgcatca cgcgggattc
     5221 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5281 cgcttctggc gcagcagcat attgaaggtc attatcaggt cgatccttcc ttattcaagc
     5341 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatt ggcattatgg
     5401 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5461 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5521 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5581 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
     5641 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5701 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     5761 tctccaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     5821 atagtccagt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5881 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     5941 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggcag
     6001 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcactgcat attgttggcg
     6061 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6121 cggcgtcact ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6181 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6241 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg
     6301 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6361 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6421 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg
     6481 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6541 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
     6601 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga
     6661 tcgtagcgtt actgttttcg gtagtttatt tgctggcagg ggaacacatc attgcgttgc
     6721 tgacgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6781 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgcg
     6841 cagccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6901 cgctgccgtg gctgggtaat catgctttgt ggctggcgtt aaccgtcttt ctggcgttgc
     6961 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7021 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7081 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7141 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7201 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7261 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7321 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7381 ggatgtacgc cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac
     7441 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7501 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7561 acgccttctg cacactcttc tttcactgcg ccgcagcgat cgcaaataaa catggctgaa
     7621 gtatgggtgg actggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     7681 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7741 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     7801 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7861 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7921 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     7981 tattgttgcc gtaatgttga ttttctgttt tgtaggtaag gtgttatgtt gccttgtcgt
     8041 accattatca acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac
     8101 tcgctattta tttaactggg tttaaacgca tacctgtagt tcagaaatta tgagcgattt
     8161 ttgatagtga ccagatggca aaagaattat aaggaggaca cggtacgaca aaaaaggtag
     8221 atgaatcatg ttgaagataa tacccggtgc aactggatac tttaataaaa cactcaattc
     8281 aaatcaattc gataatgaag atgcaatcaa agataaatta gataatagag gttcaatcaa
     8341 aggtaaatta aataatatat atggtaaatc tattgattat gcagcgctgc gtcatcggga
     8401 tataattatt gcaaaaatag atttgtttat tcaacgaatc acgcataatt tatggcatgc
     8461 acgtaaaaaa atgtgttttt aattgagcaa ataaatgatc tcaaaatgtg ggtcaataag
     8521 tatattgacg attgcactga tgaggattta aacgatcgtg actttattgc atcagttgtt
     8581 gaccgggcta tttttcattt cgcgattaat agtatatgta a
//
LOCUS       DAJTSL010000168         8598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTSL010000168 DAJTSL010000000
VERSION     DAJTSL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.168"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..183
                     /locus_tag="OO433_003480"
     CDS             <1..183
                     /locus_tag="OO433_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6051826.1"
                     /translation="EAIKRLNKLRGWRKVSRSAILREAVEQYLERQQFPVRKAKGGRQ
                     KGEVVGVDDQCKEHKE"
     gene            402..644
                     /locus_tag="OO433_003481"
     CDS             402..644
                     /locus_tag="OO433_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051827.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(974..1903)
                     /gene="fdhE"
                     /locus_tag="OO433_003482"
     CDS             complement(974..1903)
                     /gene="fdhE"
                     /locus_tag="OO433_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6051828.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLDDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1900..2535)
                     /gene="fdoI"
                     /locus_tag="OO433_003483"
     CDS             complement(1900..2535)
                     /gene="fdoI"
                     /locus_tag="OO433_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6051829.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2532..3434)
                     /gene="fdoH"
                     /locus_tag="OO433_003484"
     CDS             complement(2532..3434)
                     /gene="fdoH"
                     /locus_tag="OO433_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6051830.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3447..6497)
                     /gene="fdnG"
                     /locus_tag="OO433_003485"
     CDS             complement(3447..6497)
                     /gene="fdnG"
                     /locus_tag="OO433_003485"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5910..5912),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6051831.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVANYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6691..7524
                     /gene="fdhD"
                     /locus_tag="OO433_003486"
     CDS             6691..7524
                     /gene="fdhD"
                     /locus_tag="OO433_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6051832.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7678..>8598
                     /locus_tag="OO433_003487"
     CDS             7678..>8598
                     /locus_tag="OO433_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCR6051833.1"
                     /translation="MKRNLLSSAIIVAIMALSLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYVDWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKIFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPY"
BASE COUNT         2050 a         2175 c         2272 g         2101 t
ORIGIN      
        1 gaggcaatta aacggctgaa caagcttcgg ggctggcgta aggtttcacg atctgcaatt
       61 ttacgcgaag cggttgagca atatctggag cggcagcaat ttccggtgcg taaagcaaag
      121 ggaggcaggc aaaaggggga ggtagttggt gttgatgatc agtgtaagga gcataaggaa
      181 tgaaaaaaat ttttcattct tgaatataaa aaaacagatg cccttattct ggtattaata
      241 caaggctgtt ttacttgaac ttataataac tgcaactgtt acatcgtatc tggaaaacgc
      301 ctcgcaaaaa taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact
      361 tttctctcca tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta
      421 tagatatggg cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc
      481 tcgaggtgca gcgtaatctt cctcgtgcgg acctattaag ggaagcggta gatcaatacc
      541 tgataaatca atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg
      601 aagatggtgt cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt
      661 caatcagatt gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc
      721 cggcctacaa agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc
      781 agcgcatcag gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt
      841 tggagggaag aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa
      901 tacgagagat ggtgtcgtca caccctatga gctgtaatcc gggcgaccag cgccacccgg
      961 aaaacgtcag agattactcc ccttcacccg gaaacaggaa cgggttgatg gaactgcggg
     1021 catagccttc ttgctccatt cgcgcgtcca gtaccagaga agcgaggtca tctgccacgg
     1081 cttcaacttt cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc
     1141 agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc
     1201 tctgttcaca gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc
     1261 agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag
     1321 aaccacaaac cgggcaatat tgacgttgct cgccgtattc agcgcgggct ttgccgggga
     1381 tcagattggc catctgcgcc cagtagagcg acagtgcagc ccagataaac ggcgctttat
     1441 cgctgctgac ggatgagaaa tcagaggcaa acagtgcgct ggccatatct tccagctcct
     1501 gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt
     1561 tcagctcagc aatcagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt
     1621 gaatatccag cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca
     1681 tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc
     1741 gcaggtaatc atccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt
     1801 tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct
     1861 cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt
     1921 tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac
     1981 ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac
     2041 catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat
     2101 caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag
     2161 cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg
     2221 cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat
     2281 atcatcccga ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc
     2341 aaacataacc acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat
     2401 ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgctaggat
     2461 gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt
     2521 gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca
     2581 tccgcacggt tcggaccgac acccacgtag tggaagatac tggctgcgaa ggtagccgca
     2641 aagccaacag ctgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt
     2701 tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac
     2761 ataacgtgtg taccaccgac gcctgccgga tcgtacaggc ccgcattgtc gtaaccgcgg
     2821 gttttcagct ccgccacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag
     2881 tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg
     2941 tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg
     3001 aacggacagc ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg
     3061 ataccgttgg catactgaat aattgcacct tccgccgggc acgctttcag acagcctgga
     3121 tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc
     3181 acttccgaga agcgcatcac cgtccacgat ttggcactta aatcattggg gttgtcgtac
     3241 accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga
     3301 caggctttac agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag
     3361 tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttga
     3421 gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta
     3481 aactccggcg tctgcgtgtt cgcatcacca acgaatggcg ttaaagtatt ggcaataaag
     3541 ccttttttag ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct
     3601 ttgccgttcg ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag
     3661 ccacggttgg aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc
     3721 gactccccga tttccacaaa ctgctctggc tgcaaaattg cgttcaacag cgcgtgtttg
     3781 gtccagtagt ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg
     3841 gctttaccca atgcttcggc gtcgtcttta aagatacgtg cagccgggtt cgagataacg
     3901 tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac
     3961 ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata
     4021 aacggcccga cgccgctacc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc
     4081 cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga
     4141 tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc
     4201 agcgtgttac cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc
     4261 ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat
     4321 tgggcgaacg agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg
     4381 gtaatatcgg ccagcgcctt accgttgctc tccatcgcta cttcttccga cttcggctca
     4441 tgcggaatgg cgtagttcca ggtcatgttc agcacctgat ccgggttcgc gccaccctgt
     4501 tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcacc gtcagtcagc
     4561 gcaatccccg gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg
     4621 attgaaccgt tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc
     4681 ttcgacgagt caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg
     4741 ttcagcgggt cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt
     4801 ttgttcggga atgaggcaac agggttaaaa ccctggcaga tatagccatt gaccttgccc
     4861 tctttcatca tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag
     4921 tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag
     4981 acgaagaatt tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc
     5041 gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa
     5101 cctggcaggc tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg
     5161 cgcagggcgt taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc
     5221 gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg
     5281 aacgaggcag ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac
     5341 gcgtcttttg gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc
     5401 tgtttcagca agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag
     5461 ccgttttcgt ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca
     5521 tagccggtga aaaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg
     5581 gtataggctt cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg
     5641 cctgacagga aagcaatgtc agtaccggaa cgaatagggg catagtagtt agctaccgcc
     5701 gccgtacgcg taaagcgagg atcgatcaca atcagcttcg cgccgttgtg aattttggct
     5761 tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg
     5821 acgaggttgg cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt
     5881 ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc
     5941 atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa
     6001 gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca
     6061 atgtagttag catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc
     6121 caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg
     6181 ctttcggagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg
     6241 accgggtgat ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg
     6301 tcaccgaggc tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg
     6361 gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca
     6421 aaacctagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc
     6481 cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc
     6541 gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc
     6601 cagaattggc tcgatttccc tccatccagg aagggtttgt aacagaatac cataatgttg
     6661 gtgtgtgtgt tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa
     6721 aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa
     6781 cacccacggt tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt
     6841 tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg
     6901 ctttccgagg ggattatcga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc
     6961 tgtaatggtc ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag
     7021 cgccgccggg cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat
     7081 gacatcggaa aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg
     7141 gatgatgcat tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact
     7201 cacgccgctg cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt
     7261 cgccatgtgg cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag
     7321 caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg
     7381 tgcggcgtag agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct
     7441 gagcgctgta atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc
     7501 catccgcagc gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg
     7561 acttattcat ttcgtgaatt ttattatttt tatttataaa taaaacgcgt gtagtggcgt
     7621 tttctttttt catttattat gttcactctc ttatctatat atttataagg caattaaatg
     7681 aaaaggaatt tattatcttc cgctattata gtcgccatca tggccctcag tctgacgggt
     7741 tgtgatgata aaaaagccga aacagaaacg cccccgcctg ccaatagtca acctgccgca
     7801 ccagctcctg aagctcccgt tgcaaaagca gaagctaaac ctgaaacacc tgcgcaaccg
     7861 gtggtcgatg aacaagcggt tttcgacgaa aaaatggatg tctatatcaa gtgctataac
     7921 aagttacaga tccctgtaca gcgcagtctg gcgcgttatg ttgactggct gaaagatttt
     7981 aaacaggggc ctaccggtga agagcgtact gtttatggca tctacggcat tagtgaatcc
     8041 aacctcgctg agtgtgaaaa aggcgtaaaa agtgctgtgg cgttaacgcc tgcgctgcaa
     8101 ccaattgatg gcgtggcggt gagttatatc gatgctgccg tggcgttggg taataccatt
     8161 aacgaaatgg ataaatatta cacccaagaa aattataaag acgatgcgtt tgcgaaaggg
     8221 aaaacgctgc accagacatt cttaaaaaat ctggaagcct ttgaacctgt agcagaatct
     8281 tatcatgcgg cgattcagga aattaacgat aagcgccagc ttgcggaact gaaaaatatt
     8341 gaagaaagag aagggaaaat attccactac tattctctgg cagtcatgat ttcagcgaaa
     8401 caaattaata acctgatatc gcaagagaag tttgatgttg atgcggcaat gaagaaagtg
     8461 tctgaactgg aaacgctggt ggcgcaggcc aaagaagcgg ataaaggcgg catgaatttc
     8521 tcgtttatta attcggcagg ccagtatcaa ctcgaggcta aaaaatacgt tcgccgcatc
     8581 agagataaag tcccgtac
//
LOCUS       DAJTSL010000169         8527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTSL010000169 DAJTSL010000000
VERSION     DAJTSL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.169"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            97..972
                     /locus_tag="OO433_003488"
     CDS             97..972
                     /locus_tag="OO433_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCR6051834.1"
                     /translation="MFNRGRNKADKVDIDLSHSNLAPGRLCADGQYRQIVTVEDAVRG
                     ACNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVWTDFHALA
                     LRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGMDFRAQAD
                     AIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVLKAYDIIS
                     HRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATMHALFNEP
                     LQGESANTSNIVEIF"
     gene            972..1568
                     /locus_tag="OO433_003489"
     CDS             972..1568
                     /locus_tag="OO433_003489"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051835.1"
                     /translation="MGKSKKNRAAATKQTQHKNQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILISTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRR"
     gene            1602..2003
                     /locus_tag="OO433_003490"
     CDS             1602..2003
                     /locus_tag="OO433_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6051836.1"
                     /translation="MYMTDAAQNQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGL
                     QIIPLSEVAAKDEFLNIKNVSRDDMMAAHRVPPQMMGIMPNNVGGFGDVEKASLEFVR
                     NELIPLQKRMMEINKLVSENIVKFDSYKLAT"
     gene            complement(2110..2991)
                     /locus_tag="OO433_003491"
     CDS             complement(2110..2991)
                     /locus_tag="OO433_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051837.1"
                     /translation="MKWAIEMNKLKRPVFKDYSAFKKLSANKRLKHHSVLNGQDTFIL
                     NAYRSYIKNKGYLNVNSCGVLSNNIAEALRYYYNNPAKCLSIIDDIRAANSNSLCPMC
                     GSMHSGTLDHVLPKENYPEFSLFTKNLVPACKCNTFKSTTIANSAGHRMLHPYFDNIL
                     KQRLICANFSKLGRTPTIDVKIIIDPMSPEYNNVKYHLDNVVIKNNIKGYLSEQWESF
                     YLYPELVIRGLNKSLSTYSDVYELLTRELLLLDEKHKGKNNWNSVFVAGLIRPDVIRW
                     ILCELHLGNRSPDGRLI"
     gene            complement(2975..4624)
                     /locus_tag="OO433_003492"
     CDS             complement(2975..4624)
                     /locus_tag="OO433_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6051838.1"
                     /translation="MLVKKNKNYSLYFLITNNQQISDSGNYIKIVPSRSDWNDFGYLS
                     RVEISIFNKKECKDIISLNGYIGFLNSEEERNGKAELIRRVKKKEESNLKDEGFNDYF
                     VMLQDMNNYRRLVRFFGVNESKYILNFINDVVSKKSDASAENLRKKAISSDIFNKSFI
                     RESESYFTFKNAGTVLSGIQYEELGNLSQHIKISYSNKVNEEDVSYVFNFDHEHDLPK
                     RISIIIGENGVGKSQTLREIALAAIKGKDNLVAVVDSNGVSIEERIQISRLIAFSPTN
                     ESKCSFPTDKRIKSLIWYKRLTLNRESKSKLNLNDLIVQLARSNDAIGYNQRWNIFVD
                     ALSVLNSNNDLVIKCGGENSPFVKLNELNSRGEESRLSLYSEIILSGEPLRLIENKCY
                     PLSSGEISFIKFCVQVCLYIENGTLLLIDEPETHLHPSFINKFMALLDNLLEKTGSSA
                     IIATHSVYLVREVFKEQVTILRRRDDGVIVSEKPRLSTFGANIGNISYFVFGESEPTQ
                     ILNKVKNNIIAKNMSWDDVESKYSEDLSLNVLTALRNEMGN"
     gene            complement(4917..5150)
                     /locus_tag="OO433_003493"
     CDS             complement(4917..5150)
                     /locus_tag="OO433_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HCR6051839.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(5161..5349)
                     /locus_tag="OO433_003494"
     CDS             complement(5161..5349)
                     /locus_tag="OO433_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051840.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            complement(5503..7917)
                     /locus_tag="OO433_003495"
     CDS             complement(5503..7917)
                     /locus_tag="OO433_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCR6051841.1"
                     /translation="MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYAADNEQEQLRREALSDQVWERYFFYESRDPVQHEMEQDKLISRAKLAHEQQR
                     FNPDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHVGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEEG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNPWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMRQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCL
                     LAENLNQQEKDKSADGNPRTDITSMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     QWRNLY"
     gene            complement(7914..>8527)
                     /locus_tag="OO433_003496"
     CDS             complement(7914..>8527)
                     /locus_tag="OO433_003496"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HCR6051842.1"
                     /translation="REVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRGLCRYNKS
                     GHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVVYCDPPYDG
                     TFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKVKR
                     SIGVAAGEGKSATEIIAVSGPRCWVGFDYSRGVDSSAVYGVRA"
BASE COUNT         2302 a         2115 c         1762 g         2348 t
ORIGIN      
        1 accttaaaat caccatcata cactaaatca gtaagttggc agcattaccc accatccagt
       61 ctgacccaca gtgcttatcc gttctggtcc ggtgcgctgt tcaaccgtgg gcgcaacaaa
      121 gctgacaagg tggacatcga cctgtcccac agcaatctgg cccccggccg gctgtgcgca
      181 gacgggcaat accgccagat agtcaccgtg gaagatgcgg tgcgcggcgc ctgtaacctg
      241 ttcgaccttg accagttgcg catggagtac agccctgacg aataccagaa cctgctgatg
      301 tgcgagttcg tggacgatct cgcgtccgtg ttcccgctca gcgaattgca ggcgtgcatg
      361 gtggacagct gggaagtctg gaccgacttt catgcactgg ccctgcgccc gtttggctgg
      421 cgcgaagtgt ggatcggtta tgacccggca aaaggtacgc agaacggcga cagcgccgga
      481 tgcgtggtgg tggcaccgcc agccgtgcca ggcggtaagt tccgcattct tgagcgtcac
      541 cagtggcgcg gaatggactt tcgcgcccag gctgacgcca tcaaaaaact gactgaacag
      601 tacaacgtga cctatatcgg catcgactca accggcgttg gtcacggggt ttacgagaac
      661 gtgaaagcgt tttttcctgc cgtccgggag tttgtctaca accccaacgt taaaaacgcc
      721 ctggtactca aggcctacga cattatcagc caccgccgtc ttgagtttga cgccgggcac
      781 accgacattg cgcagtcatt catggcaatc cgtcgcgcca ccaccgccag cggcaaccgc
      841 ccgacctatg aagccagccg cagcgaagaa gccagccatg ccgatctggc ctgggcaaca
      901 atgcacgcac tgtttaacga accgctgcag ggcgagtccg ccaataccag caatattgtg
      961 gagatttttt gatgggaaag agtaagaaga accgcgctgc ggcgacgaaa cagacccagc
     1021 ataaaaacca gacttcagcc gaagcattca gcttcggcga tcccgttccg gttctggacc
     1081 gccgtgaact actggactat gtggaatgcg tacagacaga tcgctggtat gagccgccag
     1141 tgagttttga cggactggcg cgcaccttcc gcgccgccgt gcatcacagt tcaccaattg
     1201 cggtgaaatg caacattctg atcagtacct acatccctca cccgctgctc agccagcagg
     1261 ctttttcacg ttttgtgcag gattatctgg tatttggtaa cgcttacctg gagaaacgca
     1321 caaaccggtt cggagaggtc atcgctcttg agcctgctct ggcaaaatac acccgacgcg
     1381 gattagacct agatacctac tggtttgtgc aatacggcat gaccacgcag ccgtatcagt
     1441 tcacgaaagg aagcatcttt catctgatgg aaccggacat caaccaagag atctacggcc
     1501 tgccaggtta cctttctgcc attccgtcag ccctgctcaa cgagtccgcc acgctgttcc
     1561 gccgatagta ttacattaac ggcagtcatg caggcttcat catgtacatg accgatgccg
     1621 cgcaaaacca ggaggatgtg aacaacctcc gcaatgcgat gaaaagcgcc aaaggaccag
     1681 gtaacttccg caatctgttt atgtactcgc ctaacggcaa aaaggacggg cttcagatca
     1741 tcccgttgtc agaagtcgcg gcgaaggatg agtttctgaa catcaagaac gtgagccggg
     1801 atgacatgat ggcggcgcat cgtgtgccac cgcaaatgat ggggattatg cctaataatg
     1861 tcggggggtt tggggatgtg gagaaggcga gcctggagtt tgtacgtaac gagttaatac
     1921 cgttacaaaa aagaatgatg gaaataaata aattggtaag tgagaacatt gttaaatttg
     1981 atagttataa actagctact taacgtaatc actaaatgag gttctttaac acagctaaaa
     2041 aatttattaa tagccagcag tttttatgaa tttcttcagc gtgcagatac acgctgaaga
     2101 tgcaaaaaat catatgagac gaccgtctgg acttctgtta cctaaatgta attcacagag
     2161 tatccaccta atcacatcag gtcgaattaa cccagctaca aaaaccgaat tccaattatt
     2221 tttaccttta tgtttttcat ccaacagcaa caactcgcga gtcaacaact catatacatc
     2281 gctataagta gaaagactct tattcagtcc tctaataaca agctcaggat ataagtaaaa
     2341 cgactcccat tgttcagaca aatacccctt aatattattc ttgataacaa cattatcaag
     2401 atgatactta acattgttat attctggtga cattgggtca attatgattt ttacatcaat
     2461 tgtaggtgtt cgccctaatt tagaaaaatt agcacatatt aatctttgtt ttaatatatt
     2521 atcaaaatat ggatgcagca tccgatggcc tgcagaatta gctatagtag tacttttgaa
     2581 agtattacat ttgcaagcag gaaccaaatt cttagtgaat aaagaaaatt ctggatagtt
     2641 ttccttgggt aaaacatgat ccaaagtccc gctatgcatt gaaccacaca ttggacatag
     2701 agagttagaa ttagctgctc ttatatcatc aattatagat aaacactttg cgggattgtt
     2761 ataataatat ctcaaagctt cagcaatatt gttactcaat acgccacaag aattgacatt
     2821 aagataccct ttattcttta tgtacgatct atatgcatta agtataaatg tatcctgtcc
     2881 attcagaaca gaatgatgtt ttaatctctt atttgcagat agttttttaa atgcagaata
     2941 atctttaaaa acaggtcttt ttaatttatt catttcaatt gcccatttca tttctcaacg
     3001 cagttaatac attaagtgat aagtcctcgc tatatttaga ttcaacatca tcccaagaca
     3061 tattctttgc aattatattg ttttttactt tatttaaaat ttgagttggt tcactctcac
     3121 cgaaaacaaa atatgaaata tttcctatat tcgccccaaa agtactcaat ctcggttttt
     3181 cgctaacaat taccccatca tctcgtcttc tgagtattgt cacttgctcc ttaaaaacct
     3241 cccgaacaag ataaaccgaa tgcgttgcta tgatagctga agagcctgtc ttttctaata
     3301 aattatcaag caatgccata aatttattaa taaatgaagg atggagatgt gtttctggct
     3361 catctattaa tagaagagtc ccattctcaa tatacaaaca gacttgtaca caaaacttaa
     3421 taaacgatat ttccccacta ctcaaagggt aacatttatt ttcaataagc ctcaaaggct
     3481 cgccagagag aataatttca gaatataatg atagtctaga ttcttcgcct cgagaattaa
     3541 gctcatttaa tttaacaaat ggtgaatttt caccgccaca tttgattact aaatcgttgt
     3601 tactatttag aacactcagt gcatcaacaa atatattcca cctttgatta tagccgatag
     3661 cgtcgttact cctcgctagt tgcactatta aatcattcaa gtttaatttg gattttgatt
     3721 cacgatttaa agtcaatctt ttgtaccata tcaatgattt tattctttta tcggttggaa
     3781 atgaacactt actttcattt gttggagaaa aagcaatgag cctactaatt tgaattcgtt
     3841 cttcaatgct cacaccgtta gaatcaacta cagccaccaa attatccttt cctttaatcg
     3901 cagccaatgc gatttctcta agcgtttggc tttttcctac accattctcg cctataatta
     3961 tggaaattct ttttggtaaa tcatgctcgt gatcaaaatt aaaaacataa gatacatctt
     4021 cttcattaac tttattcgaa tagctaatct tgatgtgctg tgataaatta cccaactcct
     4081 catattgaat accggaaaga actgttcctg catttttgaa agtaaaatac gactcagact
     4141 ctctaatgaa agatttatta aagatatcag aagagattgc ttttttcctc aaattctctg
     4201 cactagcgtc acttttctta gaaacaacat cattaatgaa atttaatata tatttggatt
     4261 cattcacccc aaaaaatctt actagtcttc gataattatt catatcctga agcatgacaa
     4321 aataatcatt aaacccctca tcctttaaat tagattcttc ctttttcttc actcgcctta
     4381 tcagttctgc ttttccattt cgttcctcct ctgaattcaa aaaaccaata taaccattca
     4441 gtgaaattat atccttacac tcttttttat taaaaataga tatttcgact ctactcaaat
     4501 atccaaaatc attccaatcg cttcttgatg ggactatttt tatataatta ccagaatcag
     4561 atatctgttg attatttgta attaaaaaat ataaactata atttttattt ttcttgacta
     4621 acattattac cctctcactt cattagtgat gatttatttt ggaattattt tacacactta
     4681 tacctagcgc gcaatcgtat ccccgccacg cctgcccgct ttatgtagag gttttcatgc
     4741 acctgcatga tctacgcaaa agcccgccag ttctggcggg ccttagcaaa aacgatcctc
     4801 aaacgatcat gcgatctcat gcggcataga catgcactac agagctaacg cctcgcaagg
     4861 gctcgttgtt caaccttgct gacgccagaa gctagttcag acgccagcaa cgtttcttaa
     4921 tgcagccagc tgtcgtcttc ccacaccttc tgcataattt tcatcacttg ttttctttct
     4981 tcgtccagtt gcaatccagt aagttccaca ccgttagagc tacctttgcg gatacgaatt
     5041 accgttttgg gatacagagg gcgcagattg cggtaaagct cggattcaag agcgtccagg
     5101 gtagactggc taatcttctg ctctttatcg atcattattt caatgcgcat aaaagtcacc
     5161 tcagctgatg acatccattg agcggttgta ttcgtgggtt ctgatttttg ccatgagttc
     5221 atcagtcaat tcagaaaccc actgcagagc cagccccttc tcttcatcac tacactcact
     5281 agccgctaca agcttaagaa aaaaatcaat gcgctggagc ttcaaagact ccaaaaaata
     5341 gtcctgcatc tttcctccta tgacaccaca cgcaatactg tatgcataac cactgtttat
     5401 atttacagca tataataatc ttactgatgt aaaacgtttt tttacgttca tcagcctgat
     5461 atgcctggta ttattaagag cacgaattgt taacccgcgt aattaataca ggttccgcca
     5521 ctgatcatct tcctgcaaac gctggttccg atagaagata cgcaggcctg ctcctgacgg
     5581 aatactgcct ccgcgaagga gtaaatcgac ctctttctcg ctgccatcaa atcctctgga
     5641 cttcagctca tacacgagct gcagtcgctg atggtctgta attcgctgtt tgtagtcttt
     5701 acgccgtttc ggtttcacca ggcgtaacct tgctgccagt tcccggcgct cttttttgct
     5761 catactgtgc aggtaatcgt gcaactcctt gtcatccatg ctggtaatgt ccgttctggg
     5821 gttcccatca gctgatttat ctttctcctg ttggttcaaa ttttcagcaa ggagacagtt
     5881 attgccacga gtccaagggg cgcaagcgcc ctggtcggct gccgcctcct gaacgtcaac
     5941 ggctttacga accattttcc acttcactgc atgagtgcag atcttgcctt ctgcaatggg
     6001 tgaccagatg ccataaatac gaataccgtg atcgccatag gcggtcggct cttcattgat
     6061 ttcataagcg gttctgatca agtgatattt gcgaggaacc agcacgccgc cctgcctcat
     6121 aatgtaggtg gcaaaacagc cagcatcagc agcagccagg atggcatcaa ggcgcgggtt
     6181 atccagtacc ggcgcacctg cttttttgtc accctgctgc cttgccgcct gaccagccaa
     6241 caaccgcagt tcacggtaag cctgacgccc tggaatacca aagaagcgga attgctgaac
     6301 acgatgcaga gacgcccagg gattaacgta ttcagcgtta tcacgcaggg atttacccgt
     6361 ttccttgctg atctcgccag ccagaccacg cccgtcaatg ttcttactga tgtatttcgc
     6421 gatgtagctt gtcggcgttc ctttgcgcgg gtttatcagc tcagatttaa agcgtggacc
     6481 agtgttatta cccagttcct cgcggtcttc acggatggca aacttacgca acaatgcagt
     6541 aatggcgcgg cggtcttttt tgcgcataaa acacaacagg tgccagtgaa ctgtaccgtc
     6601 atgatgcggc tcagccaccc gcacgccata ccagcgcaac ccggctttgt gcatcgcctt
     6661 acgaaatgca gcaaacatgc cgaccagata atcgctgctt tgtcttaccg tcgcatttgt
     6721 ccaggttggg tttggtctgc cgttattgag cgtggaatgg aaacgtgacg gacaggtaat
     6781 ggtgtagaaa acggcgcagt caccgcgcat ttccgcgata agctccagac ctttaacaca
     6841 ggccatcatc tcattgcggc ggtgcgccgg gttgctgctg ctggcgttta ccacgtcttc
     6901 catatccagc gtgtcgccct cttcgttcac cagttcatga gaacggaaaa actccaatga
     6961 cttgcggcgc tgctcacgtt tatgcatcac ggcttcatag ctgacatagg gagatgcttt
     7021 tttgctgacc aggcagacag cacgcaactg ctcttcccgc cattcgcaac gcattttcca
     7081 taatttccgg taccaccagt cggcgcacaa catacgcgcc agcgaacccg gaatgagttc
     7141 atagggcacg ggtttacggc ggtttctttt ccgacggagt tgctcaaacg caggcgggat
     7201 gacatccaga cgcagggttt ccgctgccac cttttcccat gtcttgcgga tttcttctgg
     7261 cttaacgtca tcggtggcat acaaatcacc acaagctgca tcaaggcaca tgctcatatg
     7321 cgcagcgaca agggtagaca ggcgtttcac ctgatcctga ctcatttcag gcagaatcag
     7381 caggccgtcc agccctacat ggcttgccat aaaacgaaaa gatgcagata gctggctgtc
     7441 gcgtacatgc tccagtcgtt ccagacatgg cttaatcgtc tcacgtaaat agcgggaata
     7501 agcctttggc ctgcccaggc tgctgaagta ttcaatacgt tgcatcagcg gcttgctgat
     7561 atgggaaggc tgggcgttga cgtccgccag aatgaccata tccggattaa aacgctgctg
     7621 ctcatgcgcc agctttgccc ggctaatgag cttatcctgc tccatttcgt gctggacagg
     7681 atcacgtgat tcataaaaaa aataacgctc ccagacctga tcactcagtg cctcgcggcg
     7741 caactgttcc tgctcgttat cggcagcgta cagagtgatc aggtttgaaa gcgcagaaac
     7801 cggcgcaact tccgccgggt ccagataagg gttaatggcc tttttcgggc tgttccatga
     7861 gaacgctgcg gcagcctcgt taaagccgca gcagttgttc atatcggcat ggctcatgca
     7921 cgtactccgt acacggcaga actatccacg ccacgcgaat aatcaaatcc cacccagcag
     7981 cgcggcccgg aaacagcaat gatttctgtt gctgatttac cctcgccagc tgccacaccg
     8041 atgctgcgtt ttaccttgat atagtggtga gtaaaattgc gatacagcga acggatcagg
     8101 gatgtgtcac tgttagaaac aatgaccgga tgtccttctg atgaccgatg ttcaagaacg
     8161 gatgctaggt gatactggtc atcttcagtg aaaccatcag tgtgatagcc ggaaaacgta
     8221 ccgtcatacg gtggatcgca atacaccaca tcccccgcct tcaacatcgc cagcgtttca
     8281 tcaaagctgg cgcagataaa cgttgctcgc tgggcttttt ctgcaaatgc gcgaatttct
     8341 ttttcaggga aatacggatt tttataattg ccgtagggaa tgttgaaatg cccgctcttg
     8401 ttatagcgac ataaaccacg gtaaccgtga cgattgagat acaggaaata taccgctttc
     8461 atgaaatcag taatttcagt tgagtaatta aactcctgcc ttatgttgta ataagccacc
     8521 tccctgt
//
LOCUS       DAJTSL010000170         8456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTSL010000170 DAJTSL010000000
VERSION     DAJTSL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.170"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="OO433_003497"
     CDS             complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="OO433_003497"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6051843.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKHWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="OO433_003498"
     CDS             complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="OO433_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6051844.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGGAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="OO433_003499"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="OO433_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6051845.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMLVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OO433_003500"
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OO433_003500"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6051846.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLVAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OO433_003501"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OO433_003501"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6051847.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7827..8141)
                     /locus_tag="OO433_003502"
     CDS             complement(7827..8141)
                     /locus_tag="OO433_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6051848.1"
                     /translation="MNVQTFFKLSYARQFFFPFIAIAGSTAQGGVIHFYGQIVEPACD
                     VSTQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIE
                     YN"
     gene            complement(8258..>8456)
                     /gene="csgC"
                     /locus_tag="OO433_003503"
     CDS             complement(8258..>8456)
                     /gene="csgC"
                     /locus_tag="OO433_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6051849.1"
                     /translation="LREGGSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVV
                     TVSDGQALHLSQQWPPSSEKS"
BASE COUNT         2106 a         2211 c         2004 g         2135 t
ORIGIN      
        1 gcataacgcc ggtattttta ttggctcgtt gtttttgccg gatgcggcgt gaacgcctta
       61 cccaacctac aaaatcttgc aaaatcaata aattgcagga acgatgtagg cctgataagc
      121 gtagcgcatc aggcaactac gtttttattg cgaagccgca tccggtttac gcaatgccag
      181 tggattgagc tttatccctt cgtaataact cacccatgaa gaatatctct ccggggaatt
      241 ccagacacgg aaatgcagac gggccatcgt caccggatcg cttagcagca ccaggcgacg
      301 atcgcgattc agcttctctg gcgtctcgtt aagcgcctgt tcaacgtggc ggtcacgggc
      361 gatttccagc accttactgg cgcggtgacg cgcggtcgcc attgcggttg ccagagcgtt
      421 aaatgacggg ttaaacaccg cgtgcataaa accatcatca agggagcgtt gacgattcat
      481 ctcaaggaag cgatcggtat caaccagcac ctgcggcggt gaatactctt ccgggatcag
      541 gaacagtttc cagtgtttgg ttcgcagacc aacggtggca cggctggaaa tcaccgaaac
      601 aaacggtgac aggatcaacg agaagacaat cggtgccagc cagaacagga aacgcagatc
      661 cagccacgcc atcccaacag cccacactaa ccctaacagc agctgtgagc cgtggcgttt
      721 gaacgcttca ccccaggaag tggagtcatc atcacgctgc ggtgaattcc acaccacttc
      781 ccagccaagg aacgcgctga caacgaagac cgtatggaac agcatgcgta ccggagccag
      841 cagcacggaa aaaagcactt ccagtagcaa cgataatgta acgcgccaga agccgccgta
      901 ttctttcgtt cctttgcacc agataagcaa aatgctcaat aacttcggca ggaacaacag
      961 caccatagtc gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg gccacaccgg
     1021 gaacaactgc cgtggttgca ggaagtattg cggttcggtc aacgcatgta ctacctgcaa
     1081 tgcagtagag agcgcgagga acataaacca cagcggagcg gagagataag acatcacgcc
     1141 cgtcaggaac accgcacggt gaaccgggtg catacccttc accaggaaca gacggaagtt
     1201 catcaggtta ccgtggcacc agcggcggtc acgttttagc tcatcaagca agttaggcgg
     1261 taattcttca taagaacccg ggagatcgta agcaatccag accccccaac ctgcacggcg
     1321 catcaacgcc gcttctacaa agtcatgtga caggattgaa ccggcaaagg aaccttcgcc
     1381 cggcagcgga gccagtgcgc agtgctcgat aaacggtttc acgcggataa tcgcgttatg
     1441 tccccagtag tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa acagtggccc
     1501 atacacgcgg gtcgcgaact gctgacagcg cgcatacagc gtatccatac cggacgcttt
     1561 cggcgacgac tgaatgatcc cggcgttcgg gttggcttcc atcaggcgca ccagcccgca
     1621 caaacaatca ccggtcatta ccgagtcagc atccagcacc accatgtagc tgtactggct
     1681 gccccagcga cggcagaagt catcgatatt accgctttta cgcttcacgc ggcgacggcg
     1741 gcggcgatag aaaatctgac cttcgccacc gacttcagcg ataagctcca tccaggcttt
     1801 ttgctctgcg acgcagatat ccgggttata actgtcacta agaatgtaga catcaaagtg
     1861 tttggcattc ccggtggctt ttactgattc ccacgttgca cgcaggccag caaaaacacg
     1921 gttcacgtct tcgttacaga taggcatgat caacgccgtg cgatgctccg ggtttaatgg
     1981 ttcatcgcca actgttgacg cagatatact gtatttatcg cgaccaataa gcagttgcag
     2041 gaagcccatt aacgccgtcc agaatccggc ggacacccaa cagaacagta ccgcaaagag
     2101 gatcaggata ccggtttgca gcatataagg cagaagctgc ataaaggaaa cccacaaatc
     2161 ctgaccaacc atatccatag gattaatcag cgcccaaccc tgataaggaa gaatggtctt
     2221 catataccag gtcgcgacga cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg
     2281 gatggtaccg acggtacgcc acttttgctc gctctcctgc tcttctttgg tcaaacgagc
     2341 cagatagcgc ggcgtgacat cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt
     2401 ggtacgccac gggtcgggaa acatcgagga gcgttttgct tctggcatcg ccttcagctg
     2461 atcgcgccct tcgtcgtctt taattaactg tccatcagca agtgaatctg gccaggcttg
     2521 ttccagacgc gcctttaccg agccttgcgg ggaatcatcc tcccgcgccc aggtgcggtg
     2581 ttcggcatcc agcgcctgat gtacggcgcg gatatcagtc ttcggcaatg ccgctttctc
     2641 gcttgcggcg atgggcattg cgtcaatgta ctcagttgtc ttattcattg gcaggtaact
     2701 ggtagctcca ggtttcactc aacgtctgat cggcattcac cagcgcagca cgcatttcag
     2761 tggttttctt ggcatctttc actttcacac gcatcaccag acgccagcct ttggtaaccg
     2821 ggttatagcg caccgtgctt tcaactatct caccattatc accaatgctg gtttgcgctg
     2881 tgaccggggt atcctctggc agttttttca tctctgcgcc ggtaaaatcg accacaaagg
     2941 cgatagtacc gtcaggctgg cgaatcaggt tcgactgctt cacatccccc gttgaacgac
     3001 gcgtttgttg cacccatgcg ttatctggcg catgcagttt gtcttcatca cggctgaagg
     3061 tgatggtgta tttaaagttc atctctttac ccggctccgg cagctgatcc ggcgtccagt
     3121 aagcgacgat gttatcgttg gtttcatcgt tggttggaat ttccaccagc tcaacgctgc
     3181 ctttacccca ctcccctttc ggagtcaccc atgcgcttgg acgaagatcg taacgatcat
     3241 cgagatcttc aaagcgggag aaatcacgac cgcgctgcaa cagaccgaag ccttgcgggt
     3301 tctccatcga gaagctgcta accgctaaat gtttcgggtt attcaacgga cgccagatcc
     3361 attcgccatt accggcatgg atagagagac cgttagagtc gtgcaactcc ggacgatagt
     3421 tatttgcagg cgacggttgg ttcggcccaa acaggaacat actggttaac ggtgcacccc
     3481 ccagtttgcc gactttatcg cgcagataga ttttcgactg cacatccaca accgtgtcac
     3541 gccctggcat aaccacgaat ttgtaagcac ctgtcgcgcg cggcgagtca agcaatgcat
     3601 aaatggttaa acgtttatca gtcggttttg gacgctcgat ccagaactct ttgaagcgtg
     3661 gaaattcttc acccgatggc aaggcggtat caattgccag gccgcgggca gaaaggccat
     3721 aaacctgacc tgcaccaatc acgcggaaat agctggcccc gagcatgctg acgatttcat
     3781 cgtttttatc tttgctgttg atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt
     3841 ttaccgtgtc tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga
     3901 ttcgtttgac tgcggtggca gtcacttcat ttattttgac cggggtatcg aagtacatac
     3961 cctgatggta gaactcgagt ttgaatgggg tcttcagatt gttccagtac gctttgtcat
     4021 gattaaactg gatctgctga tagtccgcgt atttcatatc gcggaaaacg gagggcaagt
     4081 tgcttttggg cgcctcatag cctttcccgg ctaaggattg agcttgcttt gcgacatcat
     4141 caatactgaa agcccagctt gaagatgtat acagggttaa cattactgca gcactcaacc
     4201 aacgcatttt catcatttgt agtttatgtt tcataattag taagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa cagaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca attttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgattatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc tggtattttt
     4801 cgttatatcc ggctactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatcccct tactgacatt
     4921 gccgcaattt attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctgtc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccgcccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt tgccgttctt
     5281 tatcctcggt gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt acccttgcag cagcattggc atttgtcgct tatttactca accaacgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttac tttgttaacg catcgctatt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttttctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac
     5941 tggctctgta taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgtcgaacgt ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa ggttttagca tgcaggctgg caccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact actttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgttt tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaacgga ctggattcca tttggcgtaa gtaacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacgggtcct attgccataa catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgccccaaa ataggcatca ccaatatttc gtcctcccac caaggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atacggcgat tgagacggga aaagtcggtg
     6841 atataaccaa gcggacgcag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cggggatggc tgtcaagcaa gcgtaaaata tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcgacta acagggcgga aaacagtaac
     7021 cgacccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggtttttac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 tgccactgac gtatagctgt ttgccgccac cagtcgcaga ctattgagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttaacacctt cttgtttctt aagtgtacag caggcttttt ggttaaatcg catttttcta
     7801 ctcagcaacg tgcgttgttc agaaacttag ttatattcga tgttcattac agccagcttt
     7861 ttctgtttat caatatactg cacttttact gaggctactt gcgcgttttt gacattgccg
     7921 ctcattaacg ctttgctgga ataggttttt cccggaacag aaccattctg tgggcagttc
     7981 atttctacgg gtgatgactg ggtgctgacg tcacatgccg gttccacaat ttggccataa
     8041 aaatggatca cgcccccttg cgcggtgctt cctgcgatgg caataaaagg aaaaaaaaac
     8101 tgccgagcat aagagagttt aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgacattt caacaaatta agacttttct gaagagggcg
     8281 gccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aataggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga accgccttcg cgcagg
//
LOCUS       DAJTSL010000171         8264 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTSL010000171 DAJTSL010000000
VERSION     DAJTSL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.171"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..77)
                     /locus_tag="OO433_003504"
     CDS             complement(<1..77)
                     /locus_tag="OO433_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6051850.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVI"
     gene            complement(88..786)
                     /locus_tag="OO433_003505"
     CDS             complement(88..786)
                     /locus_tag="OO433_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6051851.1"
                     /translation="MQDIHEESLSESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(786..1127)
                     /locus_tag="OO433_003506"
     CDS             complement(786..1127)
                     /locus_tag="OO433_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6051852.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(1120..4380)
                     /locus_tag="OO433_003507"
     CDS             complement(1120..4380)
                     /locus_tag="OO433_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6051853.1"
                     /translation="MTEDPCEDDFPVTTLKISANSQSFQSEIQRASRMGSEYYRTLQN
                     GGRHAAAAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATEHLISLADEWGSVNA
                     RLKQASQSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVT
                     EAISTGLKLSGAGVAESGSVITQFSQALAQGVLRGEEFNAVNENGDRVIRALAAGMGV
                     ARKDLKAMADDGKLTADKVVPALISQLGILRDEYAAMPETVSGSITKVENAFMAWVGE
                     VNEACGVTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTA
                     ARNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNR
                     NIAARSAAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQAWESA
                     RQYALTIDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQ
                     YVLANPGWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLALMQEKAQSIQDVLA
                     GLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRL
                     PQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYIS
                     NALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTA
                     QGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQ
                     GERLRERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQE
                     AFYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNK
                     VEWRSWASSVLQEISKVLMNAAIVNGIKTAANSMSGAGGFIGSIGSWLGGAVANAKGG
                     VYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMN
                     GSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            4425..4754
                     /locus_tag="OO433_003508"
     CDS             4425..4754
                     /locus_tag="OO433_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR6051854.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            4741..5133
                     /gene="tnpB"
                     /locus_tag="OO433_003509"
     CDS             4741..5133
                     /gene="tnpB"
                     /locus_tag="OO433_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6051855.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKVRSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTQNTLLTQ"
     gene            5140..6741
                     /locus_tag="OO433_003510"
     CDS             5140..6741
                     /locus_tag="OO433_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HCR6051856.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAGMQRSLFEEDVDADIAALTAHLD
                     KLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKMIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGDHL
                     PLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRP
                     VEKIRQWRQRYARPILEELWSWLEEQEPQCSPGKALHKAIAYALSHRVELSRFLEDGA
                     VPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDV
                     LMRLPEWPEERLAELLPLEGFTFSG"
     gene            complement(6876..7136)
                     /locus_tag="OO433_003511"
     CDS             complement(6876..7136)
                     /locus_tag="OO433_003511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051857.1"
                     /translation="MATLRELIIKIVSFRGTHIDAFFRRASSAAHTNARGLITVDLFC
                     CTEAIARQVPEAGSEVLSSVIASGREVLTRGLIAGDDVLTRE"
     gene            complement(7184..7393)
                     /locus_tag="OO433_003512"
     CDS             complement(7184..7393)
                     /locus_tag="OO433_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HCR6051858.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDVMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            complement(7489..7863)
                     /locus_tag="OO433_003513"
     CDS             complement(7489..7863)
                     /locus_tag="OO433_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HCR6051859.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(7878..>8264)
                     /locus_tag="OO433_003514"
     CDS             complement(7878..>8264)
                     /locus_tag="OO433_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HCR6051860.1"
                     /translation="FLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLD
                     KTLTVNTGALLTMSVSANGGMPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTC
                     VVTDSAEKAQSVTSVECTVTVSAAAG"
BASE COUNT         1786 a         2611 c         2016 g         1851 t
ORIGIN      
        1 cttatcacga agccgcacga ctccgcaggc gcacaccgcc gggcatgcgc cagaatcgct
       61 gattcagtct gtgtcataaa tcgggattta ctgcgaaagt ttattaatgg aaaggaaacc
      121 gccaaaattg ccgacattcc tgcgcagttc acacccgcgc atgcacttgc tgcatctgtc
      181 cttacggata tccgtggtgg gtttatcgaa ctcatccgcc acagccccgc ccgtgtaacc
      241 acactcatca gagcggtagg tccacataca ggtgttcgcc agcatgatac gaccgggaaa
      301 cagcgccccg tccgtctcgg tcggtgtagc cagcacaaac gaggccgtca tggctgtcag
      361 ctgcgacatc tgctccacca cccagcggtc actcagctcc tgctccgggt ccgcctccgg
      421 attgcccgca acgaaattca ccgcatccag aaaacgggca tacacccggc ggcggaccac
      481 cgtggccccc accagactct gcaggtcttc cgccatcccg gtgaccagac cgaacagatt
      541 ggacaccgtc agcgacggtc tggcactgct gccccggccg ttcatctcaa agccgctgcc
      601 gtcaatcggg tatgcctcat acttacgccc ctgccaggtg accggctccc ctttttcatt
      661 cagctcatta cagaaaaaat accgctcacc accctgcacc gtcaggtcaa tttcccagag
      721 taccacccgc ggtgactgct ctgatttaac cgactcgctc agactttctt cgtgaatatc
      781 ctgcatcagt tcaccacctg ctcaatcgta caactgaaat cactgtacct ggcgttatct
      841 gtgacgctcc actcccggca taccaccctc accgtccggt tatgtttcgg cggtcgccac
      901 aaaaaggcac ggtaaccacc atgccacgat aaaaactctt ccagccatcg ccgggtcgcc
      961 tcatccgtca cccggaacac cgcctgaaac gtcttcagtc gggcattaag tcccgtcggg
     1021 cggcgctgtt cataaccgtc accaaaccgt accctcgcca ccgacggttt ctcactcacc
     1081 tgcatccctt cacgcgggac cagatgcagc gtttttatct cagccactca gcattcctcc
     1141 gtcacgtcgc atggacagca tcaccgcctg cacccgctgg tcaatcagtt gcacaaggct
     1201 gcccgcagct tccggcccta tctggccatt agtcccgtca ttctgaatgg cgatatggta
     1261 gaccggggaa tacaccagac ctgcactgcc gttcatactg cccaccgctc gcacacccag
     1321 cgagccatcc gccgcccggg tcaggggcat aatggcttca ggaccggcct cccccatcag
     1381 tcccgcccct tttgcaaagg caaagtacgt gggcgtatcc acaatactgt tgctgtacgc
     1441 actcaggttt gccgaggtat acacaccgcc ttttgcattg gccaccgcac cgcccagcca
     1501 gctaccaata ctgccgataa atcctcccgc tccggacata ctgtttgccg ccgtcttgat
     1561 cccgttgaca atcgcggcat tcataagaac ttttgatatt tcctgcagca ctgatgaggc
     1621 ccagctgcgc cattccactt tatttccgtt cagcatctcc gtgatgttat tcaccatccc
     1681 tgagataccc tccgtcgcaa gctgtgctgc ctgtgaggcg taatcggacg cattatccac
     1741 ccagttactg aatccctcct gcagcccttt ctgccagtcc gcacgctgca catccgattc
     1801 ggcataaaag gcttcctgct ctttcagacg ttcactcaga tactgcgcgt tctgtgccag
     1861 agcctgtctg taaaaatcct cactgatatc cccggtctga tactgagact gaaggtccgc
     1921 atccttctgg cggaaactgt cgcggatctg ctgcaactcc cgcatgcgtt cacggagccg
     1981 ctctccctgc ccgtacccca gcagttccgc ttcatttgat gcacgagcag ccacattatc
     2041 attcttcagg gtctcttccc gggaccgcaa ctgctcccgg attttttgct ggtcaatcag
     2101 ggccgcattg cgcagcagtt cctgcttctg catctccgtc agggttttca gttcgccctg
     2161 cgcagtctgg tatttcagct tcgccagctc tgtattctgc cccgccagtg ccagttgctc
     2221 tttctgctgc ttcagcagcc gggaaaaact gtcttccgct ttttccgtct ctgattttcc
     2281 accccgggat ttgggtttat tcgcctcgtt attgcgccag gcttccaggg cattactgat
     2341 ataacgttgt ctcgcctcct gatacggatc acccacaaaa ccgaggtcat ccgccgcata
     2401 ccccagtcgg acacgctctt tttcttcccc tttcagtctg gacagggcca gctcacgctc
     2461 tgtttttgtc agggcactct gctgtttatc atccagagtg gcctgtggca gccgtaacgg
     2521 cacattcacc agtccctgcc gctgctgaag cagttcatta cccagcccca gcagacggtt
     2581 gaattccgta tgctgaccgt tcataaccag catggactgg tacaccttat tctgctctgc
     2641 cgcctgctga cgaattaacg ccacacgacg gtcttccagc ccggcaagca catcctgaat
     2701 ggactgcgct ttttcctgca tcagtgccag acgggactgc tcaacggcaa gctgctctgt
     2761 tgcctgagca agcccttccg ttacggtctt caccgaggtc agatggttta tcatgaatcc
     2821 gtcaccggtc gtccagcccg ggttcgccag aacatactga tatcctgcga ttttttcctg
     2881 cagggatttc acccgactag cctgttcatc aatcagccgg ttctgctctg tcagcgccgc
     2941 ccgtgttcgt ccttcattat ctgaggcttc aggcaaagac attgacggcg ttttatgcgc
     3001 gatttcatct atcgtcagtg catactggcg cgcagactcc caggcctgct cctgattctg
     3061 gtacagcgtg taccatgctg cagcccccag catcaccagt ccgggtacgc caccaaccag
     3121 ccccagcgca ccgctcatca gacgtgagcc caccgccgtt gtactgttca gcgcattctg
     3181 ggcggcgctt ctggcagcaa tatttctgtt caggcgttcc tgtgtggccg ccagacgggc
     3241 ctctgcagca atctgcatct ccgtcccgcg ggctgccgcc acggcctgct gagcacggta
     3301 cacggctgcc cttgcccgcg ccgtggcaat ctgcgttccc ctgaactgtg cttccgccag
     3361 tgcaacttca ttacgtgcag ccgtcacaag tcctgccgtg gcagacatcg ctccggaggc
     3421 catattgcca aagtaccggg caaccccgac ggcaaccagc gcccccacag ctgttgccac
     3481 attatcaatc tgtccggcaa caccgttcag cacgccggag agcgtttttg tcaccccgca
     3541 ggcctcattc acttcgccca cccaggccat aaaggcgttt tccaccttcg tgatactacc
     3601 ggaaaccgtt tccggcatgg ccgcatattc atcacgtaat atccccagct ggctgattaa
     3661 cgcagggacc actttatccg ctgtcagttt tccgtcatcc gccattgcct tcagatcttt
     3721 acgggccaca cccatccccg cagcaagcgc gcggatcacc cggtcgccat tttcgttaac
     3781 agcattaaat tcttcgccac gcagtacacc ctgtgccagt gcctggctga actgggtgat
     3841 caccgaaccg gattccgcaa ctccagcccc tgacagtttc agtcctgtcg aaatggcctc
     3901 cgtcaccttc agcacatcat cagcactgta accatattca cgcatcgagg cagccgaacg
     3961 ggcaaacagg gccgcattat ccgaaaacgc ggtacctgtt cgctgactga tatccatcag
     4021 cactttctgt gatgacgaga attcatccga tgactgagat gcctgtttca gacgggcatt
     4081 cacggaaccc cactcatcgg ccagcgaaat caggtgttcg gtggcaaagg caccggcaaa
     4141 tgcgccagcc attccgacag cagaaccgcg aatttccgtc aactgactgt tcagttctgc
     4201 aagggcgcgg cgctgttccc gtgcggcagc agcggcatga cgtccgccat tctgcagggt
     4261 ccggtaatat tcactgccca tacgggacgc ccgctggatc tccgactgga atgactgtga
     4321 atttgccgaa attttgagtg tcgttacggg gaagtcatct tcacagggat cttcggtcag
     4381 ttagtattac cgacatccat tcaccaactt accggagata atcgatgtca aaaccccgat
     4441 ggaccattga acagaaaagg cagcacgttg ctgcctggcg cgccagtggt cttacacgtc
     4501 agcaatactg tgaactcaac gatattccct tcacatcact tcgcgaatgg ccgcaggatg
     4561 tcgcaaaagc tgaacgccgg gcaaatgaac cgaccgttct tcccgttcat attgcgccac
     4621 cactgcatgc tgatgtacca cagcccgtca cgaatgagcc agtcacgctc ttccttcccg
     4681 gtggagtacg gatgtgctgt cagccgtcac aacttaccga tgtcttcagg gcactcaaat
     4741 atgctcagtc ctgataatgt cttcatcgca attaaacctg ttgacatgcg ccggggcatc
     4801 gactcactga cgcagtatat acaggatgaa ctccggtcga catggcatga gggagccgcc
     4861 ttcgtctttg ttaacaaagt ccgttcgcgt atcaaagttc tccggtggga taaacacggg
     4921 gtgtggttgt gtacccgccg tctgcacaaa ggcagcttcc gctggccacg tgcaaatgac
     4981 gctgcctggc acctcactcc cgacgagttt aactggctga ttgccggtgt tgactggcag
     5041 caggttaagg gacatgacct gacgaaatgg gtctggcaga atgaacctga actgcgccct
     5101 gaaaacacgc aaaatacact gctaactcag tgaaaaataa tgaatatccg tatctggagt
     5161 ggtatactcc cctgtatgga tatctccgct ctcaacacca cgaatgacat cgaaaaactg
     5221 cgtgctatgg cacttgccat ggtacaagaa gtcatgtcgg agaatgccga aaaagagcgg
     5281 gaattactgg agaaaagccg gcgcatccag cttctggaag aaatgctgaa actggttcgt
     5341 caacagcgct tcggaaaaaa atgtgaaacg ctggctggta tgcaacgctc cctattcgaa
     5401 gaggatgttg atgccgatat cgccgcgctt accgcacatc tggataaact gctcccgcaa
     5461 tcccctgaag aagatgaaaa agcgtcccgt tcacgcccga tacgcaaacc cttaccggtt
     5521 catcttccac gggtggaaaa aatgatccag ccggacactg accattgccc tgaatgtgac
     5581 gagccgctgc actatatccg cgatgcggtg agtgaaaagc tggagtatat tcccgctcac
     5641 tttgtggtga accgttatgt ccgtccgcaa tacagttgtc cctgttgcca gaaggtgttc
     5701 agcggtgaaa tgccggcaca tatcctcccg aaaagtgccg ttgagccatc agtcatcgca
     5761 caggtgatca tcaataaata cggtgaccac ctgcctctgt atcgccagca acaggtcttt
     5821 gcccgttcag atgtcgggct gcccgtcagt tcgatggctg acatggttgg cgcggcgggt
     5881 gccgcattat ctcccctggc ggcgttactc catcgcgagt tgataaaccg tccggtggtg
     5941 catgcagatg agactaccct gaagatcctg aacacgaaga aaggcggtaa atcctgctcc
     6001 ggttatctgt gggcatacgt cagtggagaa aggacgggac cgtcagttgt gtgcttcgac
     6061 tgccggaccg gacgtagcca tgagtatcct gaaaactggc ttcagggctg gggcgggacg
     6121 ctggttgtcg acggacataa agcttaccgg actctggcaa acaaagtgcc ggagatcacg
     6181 ctggccggat gctgggccca tgcccgcagg ggcttcgccg acctgtataa aatcagtaaa
     6241 gatccacggg ctgccatagc cgtgaagaaa atcgcggggt tgtaccgtct tgagaagaag
     6301 atcagtagcc gccccgtgga aaaaatccgc cagtggcgac agcgttatgc ccgtccgata
     6361 ctggaagaac tgtggtcatg gcttgaagag caggaaccgc aatgttctcc gggaaaggca
     6421 ttacacaaag ctattgccta tgcgctgtct catcgcgtgg aactgagccg cttcctggaa
     6481 gatggtgcgg tgccgctgga taataatgtg tgtgaacggg ccatcaaaaa cgtggttctg
     6541 ggcagaaaat cgtggctgtt cgccggttcg cagatggcgg gagaacgcgc cgcgcaaata
     6601 atgagcttgc tggaaaccgc gaaacgcaac ggtctggagc cgcatgcctg gttgacagac
     6661 gtcctgatgc gtctgccgga gtggccggag gagcgactgg cagagttgct gcctcttgag
     6721 ggatttacct tctccgggtg agtgatacct gccgtcaggt gttcgtgcac cgggccataa
     6781 cctgcagtcg ggagttgaac tcctgactgc aggaaatgag ccagaccagc aaccacgcgg
     6841 gctgacagcg aataagaacc tgaatcagcc cggagtcatt cccgggtcag tacatcatca
     6901 cctgcaatta atccccgagt cagtacttcg cggcctgacg cgatcacgct actcagtact
     6961 tcagagcccg cctccggtac ctgcctggcg atcgcctctg tgcaacaaaa taaatcaacc
     7021 gttatcaaac cacgagcgtt cgtatgcgcc gccgatgacg cgcgcctgaa aaacgcgtca
     7081 atatgagttc cccggaagct tacaattttg ataatcagtt cacgtaacgt cgccattcac
     7141 ctttctccgg gcgtaaaaaa accgcctcag cggttctcat cattcatgac tgtgctgcaa
     7201 agctcagcgc gtcttccagc gccgcaaacg gatccacctc cggcttatcc tcatcctcgc
     7261 cccagcagag catgacgtcc ttcagtgcaa cattcatccc ctgtgccccg aaaaccgctt
     7321 tcacgatctg tgcattacgg atatccccgc gctcatcacc cagcggggat atcctgtcga
     7381 actccatcca catcatcgcc tcgctcgcac tcaggctgtg gcgcagttcg gataaggtgc
     7441 gccccagacg gagcgcaagt cgcatcagaa agcgaatttc cgggcgggct acttttttct
     7501 ggccgactct gcatcagcga tcagttccag tgcctgacgc agcaaccggg catgtaccgg
     7561 accatagacg gccagcacct gctcacggtc gtccggagtg aacacccgct gcaggtccgt
     7621 atcacacagg acatcgcaga acagcgtcac atccgcttcc aagttacggc gggttttcgc
     7681 caccaccgac agggtatcgt catcctctcc atcaccattg agcacttcct gccacagata
     7741 ccaggcctct gccgaaggct cccgcagcac cacgctgaca ttcccccatt ccggcacctt
     7801 caccgtttta tgacgaaacc ctgacagtct ggccagcgcc agcgttttca gatccttttt
     7861 catgatgacc catcccctta tccggcagct gcgctcactg tcacggtgca ttcaacagac
     7921 gtcacactct gtgctttctc tgccgaatcg gtcaccacgc aggtatattt ccccgcatca
     7981 gcggactgcg cacctggctt actgaaggtg tctgtcgtct gcccgtcaac cggctgacca
     8041 tccttcttcc aggcgtattt atacggcggc attcccccgt tggcactgac tgacattgtc
     8101 agcagcgcac cggtattcac ggtaagtgtc ttatccagat ttttcacaaa cgccagcggt
     8161 accacaaagg acaccggttt gcctttcaga cgcagtgaga acgttgcagc caccacaccg
     8221 ttggtaccgg atgaccaggt gtgctgacgc acttccgcca ggaa
//
LOCUS       DAJTSL010000172         8209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTSL010000172 DAJTSL010000000
VERSION     DAJTSL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.172"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1662)
                     /gene="hscC"
                     /locus_tag="OO433_003515"
     CDS             complement(<1..1662)
                     /gene="hscC"
                     /locus_tag="OO433_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR6051861.1"
                     /translation="MDNAELTIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEIHASAGDSFLGGEDFTHMLVDEVLKRA
                     EVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKQEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIE"
     gene            complement(1746..2681)
                     /gene="rihA"
                     /locus_tag="OO433_003516"
     CDS             complement(1746..2681)
                     /gene="rihA"
                     /locus_tag="OO433_003516"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6051862.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHIEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTDNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(2799..3524)
                     /gene="gltL"
                     /locus_tag="OO433_003517"
     CDS             complement(2799..3524)
                     /gene="gltL"
                     /locus_tag="OO433_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6051863.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(3524..4198)
                     /gene="gltK"
                     /locus_tag="OO433_003518"
     CDS             complement(3524..4198)
                     /gene="gltK"
                     /locus_tag="OO433_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6051864.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNIFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(4198..4938)
                     /gene="gltJ"
                     /locus_tag="OO433_003519"
     CDS             complement(4198..4938)
                     /gene="gltJ"
                     /locus_tag="OO433_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6051865.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAIIMLVMTLVERKVRLPGNMGGK"
     gene            complement(5108..6016)
                     /gene="gltI"
                     /locus_tag="OO433_003520"
     CDS             complement(5108..6016)
                     /gene="gltI"
                     /locus_tag="OO433_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6051866.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            6471..>8209
                     /locus_tag="OO433_003521"
     CDS             6471..>8209
                     /locus_tag="OO433_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR6051867.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGAGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLR"
BASE COUNT         2056 a         2031 c         2046 g         2076 t
ORIGIN      
        1 ctcaatggct aaataatcgc tcgcccgccg ccgaacttca tccacgcggg ccatgtcgtt
       61 tgactgtagc gccgcatcaa aatcggtgat cacccggcca atctcttctc gctcgtcacc
      121 cagcgctcgc gcccacaact cttcctgttt ggctttaaag gtgcgattga tgagcatatc
      181 gcgcggataa attttcaatg cggataaccg cgtccgactc tcttcaatct gctgcgcgct
      241 caatgttacc gggctgtggt taatcactct ggacttaacg ctgccgtctt ccagaagcac
      301 gtcaacttca agcaacccgt tgatatcata actaaagcga atatcaatcg actgataagc
      361 cccggttttc ttcaacggca catcgaagga ttcaaccaga atgttatttt taactttgtg
      421 gttttctccc tgatagacgt taaccgtaat tgaatcctgc tccgggtgca tggtcgagta
      481 agtttctaca cgcgacaccg gtactgtggt gtttcgttca ataatcggcg agaaaatgcc
      541 ggaaacgccc tggcggttaa cttcaacgcc caacgagtaa gggcaaatat cagtgaggat
      601 tacctcttca atatcttcac tgcgtaagcg gcaggcggcc tggattgctg cgcccagcgc
      661 gacaatggtg ctcggatcgt aactttgata cggtaatttg ccaaacagac gcacggcgat
      721 tcgctgcacc agcggcattt gtgacgcgcc gccaaccagc accagactgt cgatttgact
      781 cggcttcaga cgcgcatcgc gcaacgcctg ttcaatcggc actcgcaagc gattgagcag
      841 cggcaaccac aaatcttcca gttcgttctc gtaaaattcg cattcccgcg tttcttcctg
      901 atactgccag cgaatgtgca atggcgattg attgctacat tttgccgctt ccacacaggc
      961 gtacaaggct gccagttcac tctcgttaag cgtggtcctg gcaacttccg cgcgttttaa
     1021 aacctcatcg accagcatat gggtaaaatc ttcgccaccg aggaagctat cgccagcgga
     1081 ggcgtgaatt tcaatcaccg gcgtggcgta ctcaagcacc gtaacgtcaa acgtgccgcc
     1141 accgagatca aaaaccagcg agcgggtatt ttgttgcgta tgcaggccat atgccatcgc
     1201 agctgctgtg ggttcattaa ttaagcgtac cgcatttaac ccggctaact ccgcagctaa
     1261 acgggtatgc ttgcgttgtt catcgctgaa ataagccgga acggagatca ccacatcttt
     1321 aatcggacgt tgcagaaatt cttcggcatc ttcttttaat gagcgtaata ccaaagagga
     1381 cagttctggc gcgttaaatg tgtcgctgcc taaccgccag ttggtattac tgcccattgc
     1441 gcgtttaaat aacgctgccg ttttatccgg atgcgaagta cgccgtgata cagccggttt
     1501 tccgactaaa atatgattat tttcatccat gctaattatg gatggtgtta aatattcacc
     1561 gaacttattt ggaattaatt gcgcggcacc gtctttccag acggcaatta aactattggt
     1621 agtgccgaga tcgataccaa tggtgagttc tgcattatcc attgcattta tatcttgaaa
     1681 gaaaaagaga taaacagatt aagaccaccc aggtaacgcg tggtgatctt aatcaatgac
     1741 gtgtgttaag cgtaaaattt cagacgatca gccagtaaat caacaaagcc ctgacgatca
     1801 acatcgacca ttacagtggc attcggttta ttgtcagtca gataataata atcaacaacc
     1861 gtcataccct gggtatattt cccctgtgtt tccacgccaa cccagcgctc aacagtggta
     1921 aacaactcgg gtttcaacag ccaggcgatg gtgcatgggt catgcagcgg tgcaccgaca
     1981 aagccccatt tttcgtcttt atgatattcg aggaagaaat ccagcagttc ggcaacaatg
     2041 gttgaaacag ggttgccaat cgcgcggaaa cgctcggtgt cttcaatgtg gatttgcgct
     2101 ttatgagtaa catccagacc ggccatcacc actgggatcc ctgactggaa gacaatttct
     2161 gccgcttccg ggtcaacgta aatattaaat tcagccgcag gcgtccagtt accaagcccc
     2221 attgcgccgc ccataatcac gatacgggca attttgctat gcagttccgg atggctattg
     2281 agcagcaagg caacgttagt ttgcggtccg gtagacacaa tcgtgacagg ttccgcactt
     2341 tcgcgcagcg ttttcgccat cagctctacc gccgtacagt tttgcggtgc gaacgtcggt
     2401 tccggtaatg ccgggccatc cagaccgctt tcgccgtgca cattgtccgc gataatcaac
     2461 tcacgcatta acggttttac cgcgccgcct gctaccggaa tatcggtgcg attaagcaag
     2521 gtcagcatac gcagaacatt gcgtaaggtt ttttctggtg tctggtttcc ggcggaagac
     2581 gtaattgctt tgacatcaag ctctggtgag gcgagggcga gaactattgc gatagcgtcg
     2641 tcatgacctg ggtcgcaatc taacagaatt ggcagtgcca ttgttgctcc ttgttgtgtg
     2701 cttctttgcg acaagggtaa cgccaggatg taacagatac gaggggcgaa acgataaagc
     2761 gtgagatggc gcgcacttgg gtatgcgcgc cagagtgatt aatgcaggat tttcgcgagg
     2821 aagtctttcg cgcggtccga tttcggatca tcgaagaaag cgtctttcgg cgagtcttcg
     2881 acaattttac cctcgtccat aaagatcacc cgattcgcca ctttgcgggc aaagcccatt
     2941 tcgtgggtca ccaccatcat ggtcattcct tcgttcgcca gttccaccat cacgtccagc
     3001 acttcgttga tcatctccgg atccagcgcc gatgtcggtt cgtcgaacag catcgcaata
     3061 ggatccatac acaacgcgcg agcgattgcc acacgctgct gctgaccgcc ggaaagctgc
     3121 gccggaaatt tattggcatg tgcggaaagt cccacgcgct ccagcaattt caatgctttt
     3181 tcacgagcgg gcgctttatc gcgtttaagc actttcacct gcgccagagt caggttttca
     3241 ataatcgaca aatgggggaa caactcaaaa tgctggaaca ccatcccgac gcgggaacgc
     3301 agctttgcca gatcggtttt cttgtcatta accacgatac catcgacggt gatttcacct
     3361 tgctgcactg gttcgaggcc gttgacggtt ttaatcagcg ttgatttgcc ggaaccagac
     3421 gggccgcaaa ccaccaccac ttcgcctttt ttcacttcgg ttgagcagtc ggtcagcacc
     3481 tgaaagtgac cataccattt tgaaacattt ttcagggtaa tcattatgct gtccttcttt
     3541 tcaagtagct gaccaacaac gacgcgctaa gactaataac gaaataaaca aatccggcaa
     3601 acaggatcat ctcaacctgc gtaccatcac gctcaccaat ggttgaggcg gtacggaaga
     3661 aatcggccag agataacaca tacaccagtg aggtatcctg gaacagtacg atgccctgag
     3721 tgagcagaag cggcaccatc gcgcggaacg cctgcggcag aataatcagt ttcatcgact
     3781 gccagtgagt cattcccaac gccagcgcgg cgctcgactg accacgagaa atactttgaa
     3841 taccagcacg gataatctct gaatagtagg ccgcttcaaa catcgaaaac gccaccatcg
     3901 ccgaaattaa acggatatca ttttttggtg ataatcccag cacgttttgc agaaaacctg
     3961 gcacgatcag gtaaaaccac agcaaaacca taactaaagg aatcgagcgg aatatgttaa
     4021 cgtaggcttt ggcaaaccac gctacgggcg caaagctgga caagcgcatc accgccagca
     4081 tggtgcccca caaaatacca atcacgactg ccgtgacggt gattttcagc gtgatcacca
     4141 gcccgtcgag cagatatggc agggaaggga caatggaact ccagtcaaac tcgtacatta
     4201 tttgcccccc atgttgccag gcaggcgaac tttacgttca accagcgtca ttaccagcat
     4261 gataatcgcg ttaatcaaca catacgccag cgtaatagcg gtaaacgact cccaggcgtg
     4321 ggctgagtaa tcaagcaatt tacccgcctg cgccgccata tccaccagac caatagtcga
     4381 ggcgatggcg gagtttttca ccaggttcat catctcggaa gtcatcggcg ggacgataac
     4441 gcgataagca ttaggcagca gtacgtatcg ataagcctgc ggtagcgtca ggcccatcgc
     4501 cagcgcggca tttttttgcc cacgcggcaa cgactgaatg gcggcgcgta cctgttcgca
     4561 aacacgggcg gcggtaaaca gccccaggca gagcatggat gaaaggaaaa actgaatatt
     4621 gggatccagc tcggccttaa accacatgcc gattttctcc ggcaacagct ccgggatcac
     4681 cagataccag gtaaagaatt gcacaatcaa cggcacgttg cggaacagtt cgacatacaa
     4741 cgtacccaga ccggagagaa aacggtttgg aacggtacgt aaaatgccga aaaatgaacc
     4801 gacgaggaaa gcgataatcc aggcgcagat cgacaaagcg atcgtcacct gaaaaccact
     4861 ccagatccaa ccaagatagg tggtgttgcc gaacggggct tgttgtaaaa aaatacccca
     4921 gttccagtct atagacataa atctactcca gaaaaaagag ggtagcagcg ttaactgcta
     4981 ccctcgaaga ttgttaccca gcgtattgcg gttttcaggc ccgatgggga acgaccatca
     5041 ggcgtatagt ctgtccgtgc tacgtaacaa tcgagagggc tggaatttcc gcccctggtt
     5101 cttgtaatta gttcagtgct ttgtcattcg gttctttgaa cagtgctttc atttcgtctg
     5161 acagttcgaa attcatgttc aggtttttcg gcggaattgg atttttgaac catttatcaa
     5221 accatttttc cgcttcaccg gaagtctgca cctgagcgat agtgtcatcc atcagctttt
     5281 tgaactgcgg atcatcttta cgcaacatgc aaccgtaggc ttcctgagac tgcggcttgc
     5341 cgacgatatc ccagttgtct ggtttcttcg ctttcgcacg ttcaccggcc agcagggcat
     5401 catccatcat aaaggcaacg gcacgaccgc tttccagggt gcggaaagag tcaccgtgat
     5461 ctttggcgct gatgatgcgc atattcattt tttgctcttc attcagtttg ttgagcaaaa
     5521 cttcagaggt agtgccggaa gtgacgacta cggctttgtc tttcaggtcg gcaaaatctt
     5581 tgatatcgcc gccctttttg gtcagcaggc gcgtaccgac cacgaaaata gtatcagaga
     5641 aagccgcctg tttttggcgt tcgacgttgt tggtggtaga accacattca aaatcgaaag
     5701 tgccgttttg cagcagtgga atacggtttt gtgaggtaat cggaatcagt tttacctgca
     5761 agtccggttt gttgagtttc tttttcactg cttcaacaat ggcgttggag taatcctgcg
     5821 agtaacccac cactttttgc tgattgtcgt aataagagaa aggtactgaa gattcacggt
     5881 gaccgacgac aatcacaccg tttttggcga ttttgtccag cgtactgcct gctgccgggg
     5941 cggcgtcatc tgcctgtgcc agtcctgcgg aaagcgccag ggcgaggatt gctgtggcag
     6001 gtttacgtaa ttgcatatcc aactccttta tcttctgcgt taagaacgca tggatacccg
     6061 ttgtgagtgt ttgtgttgtt atcgtctgca actttattgt gcagtgttgt gcctgttagg
     6121 taacatttag tttggctaaa tgtaaagata ttgctgtttt attgtttgtt tttgcgagat
     6181 gcgccgcacc attccgaagc aaaattctta aaccgcacct ttttggtgct accgctgatt
     6241 tgtggtggtg caacctggtt gcgtcgcggc tgatttcatc gcttacacag taaataaagc
     6301 aacttacatg ccaaaaaata cttaggggca aattaattac gattgtaatt ttaggctgaa
     6361 aaagtcatat ttcggacaga attatctttt cggcatggga cgtttgccgg gatgataact
     6421 gcacacggga aaaatgcttt ttacctgtct tttcaaggaa gaaacacctt atgtctgcat
     6481 cttcggttaa gccgttaaat gttcaattac ccgcaataac ccttatcctt tttgcactct
     6541 gtattgggat attttgttat ctcgcacaat ggatgagtta tgaagaggtc gatcaatccg
     6601 cacttatcca tctcggcgca aacgttgcgc ctcttacttt gtcgggtgaa ccctggcgct
     6661 tattgagcag tatctttctg cacagtagtg tttctcatct gctgatgaat atgtttgcat
     6721 tcctggtcgt gggaggcgtg gcggaacaga ttctggggaa atggcgactc ctgattacct
     6781 ggttattctc cggcgtcttt ggtgggctca tcagcgcctg ttatgcgtta cgcgatagtg
     6841 atcagatagt catcagcgtt ggggcatccg gggcaattat gggaatagct ggcgctgcga
     6901 tagcaacaca gcttgcttca ggtgcgggca cataccataa aaaccagcgg cgagtatttt
     6961 ctctgttggg tatggtggcg ctgacactgt tgtatggtgc ccggcaaaca ggaatagata
     7021 atgcttgtca cattggcggc ctgattgcgg gtggcgcgtt gggttggctg agcgcgcgtt
     7081 tatctgggca aaaccgactc gttacggaag gcgggattat tgttgcgggc agtcttcttc
     7141 tgaccggggc tatctggctt gcgcagcagc agatggatga gtcagtttta caagtcaggc
     7201 aaagcctgcg tgaagcgttt tatccacagg agattgaaca agagcgacgg caaaaaaagc
     7261 agcagttagc ggaggaacgc aacgccctca gggaaacatt atccgctccg gtaagtcgtg
     7321 aacaggccag tggtgatttg ctcgctgaga ttgccgatat ccatgatatg gcgatcagtc
     7381 gggatggtaa tatgttgtat gccgcaattg aaaacacgaa cagcattgtt gttttcgacc
     7441 tcggacaaaa gaaaatcctg cataccttta cagcgcccat agcgaaagaa aagtcagtca
     7501 aacattgtgg tggctgtaaa gatcagggcg tcagatcgct ggcattaagc ccggatgaaa
     7561 agttgattta tgcgacttca tttgaagcga atgcgttatc ggtcattaac gtggcgacag
     7621 gggagattat tcagtcgatt accaccggtg cacatcctga cagccttatc ctctcgcgtg
     7681 atggcacaaa agcctgggtg atgaatcgca ccagtaatag tgtgtcagcg attgatctgg
     7741 tgacttatca gcatgtggcg gatatcccgc tggagaaata cgacggggcg gggacgagcg
     7801 gtaaaccagg cgcctgggtt atggcacttt ccccggatga aagaacatta ctggttccgg
     7861 gagcaggcag aggtaacatc gtgcggatca ataccatcac gcatcagaaa gaagactttc
     7921 ccgcaggtga tgcgcgtgga acgatatcgg cgatgcgttt tcgacctgaa aacggcgagg
     7981 ttatttttgc agatagtcag gggatttcac gtataagcgt aggggatcaa caagccagca
     8041 ttatgacgca atggtgtagc aggagcgttt attccgttga gggtattagc ccggacggtc
     8101 aatatttagc gttggtgtca tatggcctgc aaggttatgt catcctgctc aatattaatg
     8161 ccgggcagat tattggcgtt tatcctgcca gctacgttaa tcaccttcg
//
LOCUS       DAJTSL010000173         8162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTSL010000173 DAJTSL010000000
VERSION     DAJTSL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.173"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..783)
                     /locus_tag="OO433_003522"
     CDS             complement(<1..783)
                     /locus_tag="OO433_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6051868.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
     gene            875..961
                     /locus_tag="OO433_003523"
                     /pseudo
     CDS             875..961
                     /locus_tag="OO433_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1262..1351
                     /locus_tag="OO433_003524"
                     /pseudo
     CDS             1262..1351
                     /locus_tag="OO433_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            1375..5889
                     /locus_tag="OO433_003525"
                     /pseudo
     CDS             1375..5889
                     /locus_tag="OO433_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            5890..7539
                     /locus_tag="OO433_003526"
     CDS             5890..7539
                     /locus_tag="OO433_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6051869.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TTVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIHTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            7544..>8162
                     /locus_tag="OO433_003527"
     CDS             7544..>8162
                     /locus_tag="OO433_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051870.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQV"
BASE COUNT         1993 a         1964 c         2290 g         1915 t
ORIGIN      
        1 gcgagtcgac aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat
       61 actgtctggc aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg
      121 aagttcgggc agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg
      181 aaggggcggt agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg
      241 taattctggt aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg
      301 aagatcaggt agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg
      361 aagagaaggt aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg
      421 cggaagagcc gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg
      481 agggagttct ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg
      541 gggcaggtat gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa
      601 agccagacat attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc
      661 cttggcccag ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa
      721 acgggatata catcctaaac cacaaaagga gcggaaaaaa gaaggattgt gggcaggttt
      781 catttattat tgatcctcat tgtttatata ttaatatgtt cgtgcaaatg gagtgaggat
      841 tctttaaaaa aacgctcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct
      901 ggccgaaaat gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg
      961 ttggtcgccc taccttttca tcaagccagt agtctgccca ccactgcatc atttctctgc
     1021 gcttatcgag atactgagca tggttgtaaa ttcaactaac ccagtgcgtt gacaaaaaat
     1081 tagcgcaaga agacaaaaaa tcaccttgcg ctaatgctct gtatcaggtc actaatacca
     1141 gctaagtagt tgattcatag tgactgcata tgttgtgctt tgtcgcattt tgtagtctat
     1201 tatttaatct gtaacaacga taatacaatg aattttaatt ggtttttgtg attttgtcct
     1261 tcttctgaca tggaaggaca cccgatggat acccaacgat tccaatccca atttcattgg
     1321 catttatcgt ttaaattttc tggcgcgata gcagcgtgct tgtctctgtc tcttgtggga
     1381 acagggcttg ctaatgctga tgattcgctt ccttccagta actatgcgcc gcccgccggg
     1441 ggaacattct ttttgcttgc tgacagcagt tttggcagca gtgaagaggc gaaagtgcga
     1501 ctggaagcgc cggggcgtga ttatcggcgc tatcagatgg aagagtacgg cggcgtggac
     1561 gttcgcctgt atcgtattcc tgacccgatg gcatttttgc gccagcagaa aaacctgcat
     1621 cgcattgtgg tgcaaccgca atatctgggc gacgggctga acaatacgct gacctggctg
     1681 tgggataact ggtacggcaa atctcgccgc gtaatgcagc gtactttctc ttctcagtca
     1741 cggcagaatg tgactcaggc attacccgaa ttacatctcg gcaatgccat tattaaacct
     1801 tcccgttatg tacagaacaa ccagttttcc ccgctgaaaa aatatccctt ggtggaacag
     1861 ttccgttatc cactatggca ggctaaaccg gtcgagccgc agcaaggggt aaaactggaa
     1921 ggcgcatcca gcaatttcat ctcgccgcag ccgggtaaca tttatattcc tctcggccaa
     1981 caagagccgg gactgtacct cgtcgaggcg atggttggtg ggtatcgggc gacgacggtg
     2041 gtgtttgttt ccgataccgt ggctcttagc aaagtgtcag gcaacgagct tctggtgtgg
     2101 accgcgggta aaaaacaggg tgaagcgaag cccggctcag agatcttgtg gactgacggt
     2161 cttggcgtga tgacccgcgg tgtgaccgat gacagcggta ccttgcagtt acaacatata
     2221 tcgccagaac gttcatacat tctgggtaag gatgctgaag gcggcgtttt tgtctccgag
     2281 aacttcttct acgaaagcga aatctacaac acccgcttgt atatttttac cgatcgcccg
     2341 ctatatcgcg caggcgatcg tgtcgatgtt aaagtgatgg gccgcgagtt ccacgatccg
     2401 ttgcattcat cccccatcgt cagcgccccg gcgaagcttt cggtgctgga cgctaacggc
     2461 agtctgttgc aaaccgtcga tgtcacgctg gatgcgcgca atggcgggca gggaagtttc
     2521 cgcctgccag aaaatgccgt tgccggaggt tatgagttac gtcttgctta ccgcaatcag
     2581 gtctatagca gcagttttcg cgtggcaaac tacatcaagc cacatttcga gattggttta
     2641 gctctcgaca aaaaagagtt caaaactggc gaagcggtca gcggcaaact gcaactgctc
     2701 tacccggatg gcgagccggt aaaaaatgcc cgcgtgcagt taagtttgcg cgctcagcaa
     2761 ttatcaatgg tcggtaacga tttgcgttat gccggacgtt tccccgtgtc gctggaaggc
     2821 agcgaaacgg tgtccgacgc cagcggtcat gtggcgttaa atctccccgc cgccgataaa
     2881 ccgagccgtt atttgttaac cgtctccgcc agtgacggcg cggcgtatcg tgtcaccacc
     2941 accaaagaga tcctcattga acgcggtctg gcgcattact cattaagtac tgccgcacaa
     3001 tacagtaata gcggcgagtc ggttgtgttc cgttatgccg cgctggaatc ttcaaaacag
     3061 gttcctgtta cgtatgaatg gttgcgtctc gaagaccgca cgagccatag cggagagcta
     3121 ccgtcaggcg gcaaatcctt taccgtcaat ttcgctaaac ctggcaacta caatctgaca
     3181 ttacgcgata aagacggctt aattctcgct gggttaagtt atgccgtcag cggtaagggc
     3241 agcacggcgc atacgggtac ggtagatatc gtggcggata aaacgctgta ccagccaggc
     3301 gaaaccgcga agatgctgat tacctttccg gagccaattg atgaagcatt attgacgctg
     3361 gaacgcgatc gcgtggaaca gcagtcgctg ctttcgcatc cggcaaactg gctaacgcta
     3421 caacgtttaa acgataccca gtatgaagcc cgggttccag tgagcaattc ctttgcgcct
     3481 aacatcactt tttcggtgct gtatacccgt aatggtcagt acagttttca gaacgccggg
     3541 atcaaagttg ccgttcctca gcttgatatc cgggtgaaaa cggacaaaac ccattaccag
     3601 ccaggtgaac tggtcaatgt cgaattaacc tcgtcgctga aaggtaaacc tgtttctgcg
     3661 cagctaacgg taggcgtggt cgatgaaatg atctacgcgc tgcaaccaga aatcgcgccg
     3721 aatatcggca aatttttcta tccgctgggg cgtaacaatg tgcgtaccag ctccagtctg
     3781 tcgtttatca gctacgacca ggcgctctcc agcgagccgg ttgcgcctgg cgcaactaac
     3841 cgcagcgagc ggcgagtaaa aatgcttgaa cgtccacggc gtgaagaggt ggataccgcg
     3901 gcatggatgc cgtcactcac aaccgataaa caaggcaaag cgtatttcac gttcctgatg
     3961 cctgattcgt taacccgctg gcgtatcacc gcgcgtggga tgaacggcga cgggctggtc
     4021 gggcaggggc gtgcttatct gcgttcggaa aaaaatctct acatgaagtg gagtatgcca
     4081 acggtgtatc gcgtgggcga caaaccggcg gcaggactgt ttatcttcag tcagcaggat
     4141 aacgaaccgg tggcgctggt gactaaattt gcaggcgctg agatgcgcca gacgctgacg
     4201 ctgcacaaag gggcgaatta tatttcgctg gcgcagaata ttcagcaatc tggcttgtta
     4261 agtgcagaac tgcaacaaaa tgggcaagtg caggacagca ttagcacaaa actgtctttt
     4321 gtggacaaca gcaggcccgt tgaacagcag aaaaatgtca tgctcggcgg tggcgagaac
     4381 gcgctgatgt tgcccgagca ggcgagtaat atccggctac aaagtagtga aacgccgcag
     4441 gagattttcc gcaacaatct tgatgcgtta gtcgatgaac cgtggggtgg ggtgatcaac
     4501 accggtagcc gtctgatccc gctcagtctc gcctggcgtt cgcttgccga tcatcaaagt
     4561 gccgccgcta acgacattcg tcagatgatt caggataacc gtctgcggct gatgcaactg
     4621 gcggggcccg gagcgcgctt tacctggtgg ggtgaagatg gcaatggtga cgccttcctt
     4681 acggcatggg catggtacgc cgactggcag gccagctagg cgctcggcgt aacgcaacaa
     4741 ccggaatact ggcagcatat gctcgacagt tatgccgagc aggcggataa catgccgtta
     4801 ttgcatcggg cgctggtgct ggcgtgggca caggagatga atctgccgtg caaaacgttg
     4861 ttgaaagggt tggatgaagc tatcgcccgg cgcggaacta aagatgaaga tttctctgag
     4921 gaagacatcc gcgatatcaa tgacagcctg atcctcgata caccggaatc tccactggca
     4981 gatgcggtgg caaacgtctt aaccatgacg ttgctgaaaa aagcgcagtt gaagtccacg
     5041 gtgatgccac aggttcagca atatgcgtgg gataaagcgg caaacagcaa tcagccgctg
     5101 gcgcacacgg ttgtgctgct caatagcggt ggtgacgcta cccagacggc cgctatttta
     5161 agtggtttga ccgctgagca atccactatt gagcgcgcgc tggccatgaa ctggctggcg
     5221 aaatatatgg cgacaatgcc tccagttgtt ttgcctgcgc ctgcgggcgc atgggctaaa
     5281 cataagttaa ctggaggggg cgaatactgg cgttgggttg gtcagggcgt gccggacatt
     5341 ctctcttttg gtgacgaatt atcgccgcaa aatgtgcagg tccgctggcg tgagccggca
     5401 aaaatggctc aacaaagtaa cattccggtg accgttgaac gccagttgta tcggcttatc
     5461 cctggtgaag aagagatgag ctttattctg caaccggtga ccagcaatga gattgacagc
     5521 gatgcgctgt atctcgatga catcacgctt accagcgagc aggatgcagt tctgcgctac
     5581 ggtcaggtgg aagtaccgct gccaccggga gccgacgttg agcgcacaac atggggcatt
     5641 tcggtcaata aacccaacgt cgcgaaacag caggggcaat tgctggaaaa agcgcgaaat
     5701 gaaatgggcg aactggccta tatggtgccg gtgaaagaac tgacgggaac ggtcactttc
     5761 cgccatttgc tgcgcttctc gcaaaaaggg caattcgttc tgcctcctgc tcgttatgtg
     5821 cgttcctatg cacctgcaca gcaaagtgtt gcggcaggga gtgaatggac cgggatgcag
     5881 gtgaaataag tgaactggcg cagaatcgtc tggctattgg cgttggttac tcagccaacg
     5941 cttgcggagg agacgccgtt acaactggca ctgcgtggtg cgcagcacga tcaactgtat
     6001 cagctgtcgt catcaggcgt gaccacagtc tcagcattac ctgactcgct caccacgccg
     6061 ttagggagtt tgtggaaact gtatgtttac gcctggctgg aggataccca tcaaccggag
     6121 cagccctacc agtgtcgcgg aaactccccg gaagaggttt attgttgtca ggctggggag
     6181 agcataacgc gcgataccgc gctggtacgt tcctgtgggc tctattttgc gccgcagcgg
     6241 ttgcatattg gcgcagatgt gtgggggcaa tactggcaac agcgtcaggc gcctgcgtgg
     6301 ttagcgtctc ttactacgct gaagccagaa acctcggtaa cggttaaatc gctgcttgat
     6361 tcattagcga cgttacctgc gcaaaacaaa gctcaggaag tcttactgga tgtggtgctg
     6421 gacgaggcga aaatcggtgt cgcatccatg ctcggtagtc gggtacgggt gaaaacctgg
     6481 agctggtttg ccgatgataa acaggaaatc cgtcagggtg gttttgccgg ctggttaacc
     6541 gatggtacac cgctgtgggt tactggcagt ggcaccagca aaacggtact aacccgttat
     6601 gcgacagtgc ttaatcgggt tttgccagtg ccgacgcagg ttgccagtgg gcaatgtgta
     6661 gaggtggagc tgtttgcccg ctatccgctg aagaaaatca cagcggaaaa gagtacgacg
     6721 gcagtaaaac ccggcgtact taatgggcgc tatcgtgtga cgtttactaa tggtaaccac
     6781 attacgtttg tcagccacgg tgagacgacc ttactgagcg aaaagggcaa actgaaattg
     6841 cagtcgcatc tcgatcgtga agagtacgtt gccagagttc tggatcgcga ggcgaaaagt
     6901 acgccccctg aggcagcgaa agccatgacc gtggcgattc acactttttt acagcaaaac
     6961 gccaatcggg aaggcgattg cctgaccatc cccgacagca gcgccacgca gcgtgtttct
     7021 gcttcgccag cgaccacggg ggcgcgaacg atgaccgcct ggacgcagga tctcatctat
     7081 gcaggcgatc cggttcatta tcatggcagt cgagctaccg aagggacact ttcctggcgg
     7141 caagccacgg cccaggcggg gcagggagag cgttacgacc agatactcgc gtttgcttat
     7201 cccgacaaca gccttagccg ctggggagcg ccacgctcaa cctgccagtt attacccaaa
     7261 gcgaaagcct ggctggcgaa aaaaatgccg cagtggcggc gtatattaca aggcgagacg
     7321 gggtacaacg aaccagacgt gtttgcggtc tgtcgtttag tctcaggttt cccctatacc
     7381 gatcggcagc agaaacggct gtttattcgt aacttcttta cgcttcagga tcggctggat
     7441 ttaacccatg agtatctgca cctggccttt gatggttatc ccaccgggct tgatgagaac
     7501 tatatcgaaa cgctgacccg acaattatta atggactaat gctatgcgaa agatttttct
     7561 tccgttgtta ctggtggcac tttcgcctgt cgctcacagt gaaggtgtgc aggaagtcga
     7621 gattgacgca ccgctctctg gctggcatcc ggcagagggc gaagatgcca gtttttcaca
     7681 atcgatcaat tacccggcgt cgtccgtcaa catggcagat gatcaaaata tctcagcgca
     7741 gatccgcggc aaaataaaga attatgctgc ggcgggtaaa attcagcagg gccggctggt
     7801 ggtcaacggt gccagtatgc cgcagagagt tgaatccgat ggttcatttg cacgccctta
     7861 tattttcact gaaggcagca acagcgtgca ggtcatcagc ccggatgggc aaagccgaca
     7921 aaaaatgcag ttttactcaa cgccgggtac aggaacgatt cgtgcacgtt tacggctggt
     7981 tctctcgtgg gatacggaca ataccgatct cgatctccat gttgtcacgc ccgatggcga
     8041 acacgcctgg tacggtaaca ccgtgctgaa aaacagtggt gcactggata tggatgtcac
     8101 gacggggtac ggacccgaga ttttcgccat gccagcgcca gttcacggac gctatcaggt
     8161 gt
//
LOCUS       DAJTSL010000174         8130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTSL010000174 DAJTSL010000000
VERSION     DAJTSL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.174"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..250
                     /locus_tag="OO433_003528"
     CDS             <1..250
                     /locus_tag="OO433_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6051871.1"
                     /translation="GMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRL
                     NGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(389..1018)
                     /locus_tag="OO433_003529"
     CDS             complement(389..1018)
                     /locus_tag="OO433_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6051872.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(1141..2787)
                     /gene="fumB"
                     /locus_tag="OO433_003530"
     CDS             complement(1141..2787)
                     /gene="fumB"
                     /locus_tag="OO433_003530"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6051873.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGIAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRI
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDTCHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2865..4205)
                     /gene="dcuB"
                     /locus_tag="OO433_003531"
     CDS             complement(2865..4205)
                     /gene="dcuB"
                     /locus_tag="OO433_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6051874.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4776..5495)
                     /gene="dcuR"
                     /locus_tag="OO433_003532"
     CDS             complement(4776..5495)
                     /gene="dcuR"
                     /locus_tag="OO433_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6051875.1"
                     /translation="MSNVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5492..7123)
                     /gene="dcuS"
                     /locus_tag="OO433_003533"
     CDS             complement(5492..7123)
                     /gene="dcuS"
                     /locus_tag="OO433_003533"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6051876.1"
                     /translation="MRHSLPYRMLLKRPMKLSTTVILMVSAVQFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVVSGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYSLKTANNYKEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            7304..7534
                     /locus_tag="OO433_003534"
     CDS             7304..7534
                     /locus_tag="OO433_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCR6051877.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            7546..7818
                     /locus_tag="OO433_003535"
     CDS             7546..7818
                     /locus_tag="OO433_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6051878.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            8045..>8130
                     /gene="ghoS"
                     /locus_tag="OO433_003536"
     CDS             8045..>8130
                     /gene="ghoS"
                     /locus_tag="OO433_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6051879.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRS"
BASE COUNT         2023 a         1967 c         2077 g         2063 t
ORIGIN      
        1 agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca
       61 gtttattatg attgctctac caactctgtt tttcatggta acgctgattc tctacttccg
      121 tttctatcgc ctcaatggtg acacgctgcg caggatccag atccatctgc tggataaata
      181 tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt
      241 gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt ttatcaggcc
      301 acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgct
      361 gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag cgccgtagct
      421 ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc agcgcgcaga
      481 acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca tccgctccct
      541 gacccattgc cagcgccgcc caccactcga ttaattcata cattgcgctt atcgccagcg
      601 ccacgcagca gactaggaac gccaccattt tacgtccgcg cacgtacatc ccgcgaacga
      661 gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaagtgcccc agcttgtcat
      721 acggattacg gctcaacccc agccattcct gcacctcaaa accaacgggg actttcgcgt
      781 aggtgtattg tccgccgacc atcaggatga tggcgtgaag gaaaatgagc gtatagagca
      841 gcggcgttaa cggataacgt ctggcggtgg caagcagtag cggcacgaca ataatcaccg
      901 gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta taaatcagga
      961 ttaatgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg cgggtcatgg
     1021 tgagttcgca aatcagggaa aatactgact attcaccagc aagcgtgaaa ttacaatcgc
     1081 aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg
     1141 ttacttagtg cagttcgcgc actgtttgtt gacgatttgc tggaagaagt cgttaccttt
     1201 gtcatcgacc aagataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat
     1261 acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg ccagtaccgc
     1321 cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg tgtcggtaac
     1381 ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg attgcagcag
     1441 atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg atggataacc
     1501 ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt actgcggaag
     1561 ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc ggcccacgat
     1621 aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct gggcgaggat
     1681 ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg cctggcgcag
     1741 ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga taccttcgcg
     1801 gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc cgaccgggca
     1861 ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt atttaccgcc
     1921 aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt ccagctggac
     1981 atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt aatagtgagc
     2041 gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa tcacaaacgc
     2101 gatatggtac ggcgggcagg ctgcagtacc gaggatacgc attttctcga cgaggaagtt
     2161 tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg ttttgttggc
     2221 agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta ccgcgtacag
     2281 gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt ccagcgccgc
     2341 attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt tcgacagcgc
     2401 ttcttcatca ccgccgccgg tccacacgcg ctggcctttt ttaccgacga tgatcgcgat
     2461 gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggaat ttcttaagaa
     2521 ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa tagccgcaac
     2581 ctgtttctgg tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt gctgcgccag
     2641 cagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt cggcaacgct
     2701 aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg ggaaaggtgc
     2761 ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta tttgttattc
     2821 gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa gaacccgtac
     2881 atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa ccccggcaga
     2941 ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga
     3001 atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca agccggtgct
     3061 gaagccacga tgtatgccgg atcaacgccg atcgccaacg cgaccggaac aatcgccgcc
     3121 agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat ggcataggcc
     3181 cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat atgcgcaccg
     3241 aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat cataccggaa
     3301 cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat aataatcagc
     3361 gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg tttgccgccg
     3421 aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat tgccccgagg
     3481 aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatccagcag cgtcgcggta
     3541 tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa ctcttcgtct
     3601 ttatccagat ctttaccacg gaaccagctg aagataccga tcgccaggat accgattaac
     3661 gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc atcaaaggtg
     3721 acattaccca gcatcgcaac cagagacacg accgcaaccg acaccggact ggcgataatc
     3781 cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat gttgttctta
     3841 atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt accgcaaaga
     3901 atggtcagcg tacaggtcac aaacggcgcg acaattgaga catatttcgg gttgcggcgc
     3961 agcaacttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc
     4021 gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt acctggctga
     4081 aggtggaaga caaagaccag aatgaccaga ccgataccgc ctaataaacc cagcgcgata
     4141 ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg gatagtaaat
     4201 aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga tcacagtcac
     4261 gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg tcgttaccgg
     4321 ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa tttatgcact
     4381 gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata gtttgtactg
     4441 cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc tattttttgt
     4501 tctcagggca gatatcatta ggtttaaaga ttaatcttat ctacccattt gtgggtgaaa
     4561 atacacataa tgcgggtgac ataatagtta attaactttt gttagcgttt tgaaattaaa
     4621 aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata attatatttg
     4681 atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt ttgcattacg
     4741 gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg tttcagtagt
     4801 gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac gccataatgg
     4861 atactggtga acaagatgtg gcagttgacc agccagatga ggtatttacg gcaggaaaca
     4921 cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc ctgatgcgcg
     4981 tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg caagcgacga
     5041 ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc ctggtcataa
     5101 tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc ctcttcaaag
     5161 cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag cgaatcttta
     5221 atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca acgcgcgtta
     5281 tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc gagcaatatc
     5341 aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag cgtcgaggct
     5401 gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag ctccgcgacc
     5461 attgcgtcgt catcgataat taatacattg ctcatctgtt tgacctctcc ccgtcccagg
     5521 gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg atgctgccgc
     5581 cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg cttccttttg
     5641 tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc ggtccatcat
     5701 cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta atttcgccac
     5761 cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat gtggtaatca
     5821 gcgtcgcgac ctggtcctca ctgccgctgt ctggcagcag gctttcactg tttaaaatca
     5881 gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca gcgataaccg
     5941 gagatttgat cttacccagc agagagccaa tctcttcctt atagttattg gctgttttga
     6001 gactgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga atcacatgca
     6061 atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg accagaccgt
     6121 cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa atggcaccga
     6181 tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg tctttaatcg
     6241 taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca tctaccacct
     6301 gtgaccatga gtggcttagc gtcgacagtt tctcatcgtc ctgcgactta cggtaattca
     6361 gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc acggcaacga
     6421 cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg gagatttcgt
     6481 agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag gtgccaatca
     6541 gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg tcattgattt
     6601 gttgggtcac gcggcttaac tcaaggccgc tcaccaccac gccaatttgt ttatgatttt
     6661 catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga ttgatagcga
     6721 cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc tgaccaatac
     6781 gctgggcttc aggatgcgag tagcgaagac tttgcatatc cgtaacgaca ataaacagca
     6841 gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc tgcggttttt
     6901 tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact gccagtgcct
     6961 tgttggccag cccgtctcgt gtcatatcac tgatttgcga gaagtaaatc agatgcacca
     7021 ccaatagcac cgagaactgt accgcgctga ccattaagat cactgtggta ctcaatttca
     7081 tcggacgttt gagtaacatg cggtagggca atgaatgtct catcagcttc cttgtgtgac
     7141 aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa gaatgattgg
     7201 ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa gattccaggg
     7261 ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc aggcgctact
     7321 ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca acactgcgat
     7381 atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc tcaaacgaaa
     7441 gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga ttgatacgtg
     7501 cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga aatacgcgaa
     7561 ggccacaata aattttacat taatgacgaa cagggcaagc aaatcgctga aattgtcttt
     7621 gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga aagcctgaaa
     7681 gggcagggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg tcgggaaaaa
     7741 cgaaaaatta tcccactatg cccatttgcg aaacacgaat ttgataaaac gcgggagtat
     7801 gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg ctctccgcgg
     7861 agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc agagagcgcg
     7921 tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa agaatatctc ctatatgaga
     7981 atcatcaatc ggggttaata agttttgcgt ccccagagca ttcaatattg ataggagtca
     8041 tattatggaa ggtaaaaaca agttcaatac ttatgttgtt tcttttgatt atccatcatc
     8101 ttattcctca gtgttcttaa ggttaagatc
//
LOCUS       DAJTSL010000175         7913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTSL010000175 DAJTSL010000000
VERSION     DAJTSL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.175"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(132..863)
                     /gene="dnaQ"
                     /locus_tag="OO433_003537"
     CDS             complement(132..863)
                     /gene="dnaQ"
                     /locus_tag="OO433_003537"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6051880.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            928..1395
                     /gene="rnhA"
                     /locus_tag="OO433_003538"
     CDS             928..1395
                     /gene="rnhA"
                     /locus_tag="OO433_003538"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6051881.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1392..2114)
                     /locus_tag="OO433_003539"
     CDS             complement(1392..2114)
                     /locus_tag="OO433_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6051882.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2148..2903
                     /gene="gloB"
                     /locus_tag="OO433_003540"
     CDS             2148..2903
                     /gene="gloB"
                     /locus_tag="OO433_003540"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6051883.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            2975..4333
                     /gene="mltD"
                     /locus_tag="OO433_003541"
     CDS             2975..4333
                     /gene="mltD"
                     /locus_tag="OO433_003541"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6051884.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRDSLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4382..4978)
                     /locus_tag="OO433_003542"
                     /pseudo
     CDS             complement(4382..4978)
                     /locus_tag="OO433_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(5009..5809)
                     /locus_tag="OO433_003543"
     CDS             complement(5009..5809)
                     /locus_tag="OO433_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6051885.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6050..6964
                     /locus_tag="OO433_003544"
     CDS             6050..6964
                     /locus_tag="OO433_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6051886.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(6961..7754)
                     /gene="dkgB"
                     /locus_tag="OO433_003545"
                     /pseudo
     CDS             complement(6961..7754)
                     /gene="dkgB"
                     /locus_tag="OO433_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
BASE COUNT         2106 a         1900 c         1922 g         1985 t
ORIGIN      
        1 cgttgtgttc cgttgggaac gaggcgaata ttacggattg cctcaccctt cgtcaacggt
       61 ttttctcatc ttttgaatcg tttgctgcaa aaatcgccca agccgctatt tttagcgcct
      121 tttacaggta tttatgcccg ccagaggcag cttccgccct tcttctccac cagatcaaga
      181 cgggcttcat gagctgcaag ctcttcatct gtcgcaaaaa caacgcgtaa cttacttgcc
      241 tgacgtacaa tgcgctgaat tgttgcttca ccttgttgct gctgtgtctc tccttccatc
      301 gcaaaagcca tcgacgtttg accaccggtc atcgccagat aaacttccgc aaggatctgt
      361 gcatcgagta atgccccgtg tagcgttcgt ttactgttat ctatttcgta gcgggcacat
      421 aacgcatcga ggctgttgcg cttaccagga aacattttcc tcgccaccgc aaggctatcg
      481 gtgaccttac agaaggtatt ggtcttcgga atatcgcgct taagcaacga aaactcgtag
      541 tccataaagc cgatatcgaa cgctgcgtta tggatcacca actccgcgcc gcggatatag
      601 tccatgaact catcggctac ttcggcaaac gtgggcttat cgagcaaaaa ttcatcggca
      661 ataccatgta cgccaaaggc ttccggatcc accagccgat cgggtttgag ataaacatgg
      721 aagttattgc ccgtcaggcg acggttcacc acttcaacgg caccaatctc aatgatcttg
      781 tggccttcat agtgcgcacc aatctggttc ataccggtgg tttcggtatc gagaacgatc
      841 tggcgtgtaa ttgcagtgct catagcggtc atttatgtca gacttatcgt tttacagttc
      901 gattcaatta caggaagtct accagagatg cttaaacagg tagaaatttt caccgatggt
      961 tcgtgtctgg gcaatccagg acctgggggt tacggcgcta ttttacgcta tcgcggacgc
     1021 gagaaaacct ttagcgcagg ctacacccgc accaccaaca accgtatgga gttgatggcc
     1081 gctattgtcg cgctggaggc gttaaaagaa cattgcgaag tcattttgag taccgacagc
     1141 cagtatgtcc gccagggtat cacccagtgg atccataact ggaaaaaacg tggctggaaa
     1201 accgcagaca aaaaaccagt aaaaaatgtc gatctctggc aacgtcttga tgctgcccta
     1261 gggcaacatc aaatcaaatg ggaatgggtt aaaggccatg ccggacaccc ggaaaacgaa
     1321 cgctgtgatg aactggctcg tgctgcggca atgaatccca cactggaaga tacgggctac
     1381 caagtcgaag tttaagcttg tggtttacga cattgccggg tggctccaac cgcttggcga
     1441 attcgtggct tgtttttact ctgtttcatc gggtttagcg ttaaaggaat agtccgtttc
     1501 cgggcaacaa taagttgtaa gcaaccaagc gcaggaatat gcgcattcaa tagttttcct
     1561 ccgtgtttgt tccacgggag aacgtggaaa cggctggcgt gcagcacttc aaaattcaac
     1621 aaagagagcc agtccagttg ccgcatcaga gtaaacatcc ggctgttata gggcgaggtt
     1681 tttcgcaata ccggcacaag tttgcgtaat cccattaaac taatgggatt gaagccacta
     1741 atcaccagcc agccatcatc aatcaatacc cgatcggttt cacgcaataa acgatgcgga
     1801 tcggtgcacc acggcaatgt atgtgccagt agacaaacat caacggattt atcggcaaaa
     1861 ggaagatgaa gtggatccgc ctgcacctgg acgggcattc cttgcgcaga aacattcact
     1921 tgatgagaaa ccgcgcacgc ttcgcaattg atttctgcgc ttaaattgcc aatcttaagc
     1981 agatgaaaac catacatttt agtgaaccat gggttgagct ggcgctccag cgccttgcga
     2041 taaagctctc cccagggcaa atcgccccag cgatcaggag ccacgacagt ttgagggact
     2101 cttgccggtt tcatcacaac cttccgtttc acactaagag gtaatctatg aatcttaaca
     2161 gtattcccgc ctttgatgac aattacatct gggttttgaa tgatgaagca ggtcgctgcc
     2221 tgattgtcga tcccggagac gcagagccag tattaaacgc cattgccgcc aataactggc
     2281 aaccggaggc catatttctc acccaccatc atcacgatca cgttggcggc gtaaaagaac
     2341 tggtggaaaa gtttccgcaa attgtggtgt atggtccaca agagacacaa gataagggaa
     2401 caacacaggt agtcaaagat ggcgaaactg ccttcgtttt ggggcatgaa tttagtgtaa
     2461 ttgccacacc gggtcacact ttaggacata tctgttactt cagtaaacct tatctatttt
     2521 gcggcgacac gctgttttct ggtgggtgtg gtaggttgtt tgaagggaca gcatcacaaa
     2581 tgtatcaatc acttaataag ttaagtgcgt tacctgacga tacattggta tgttgtgctc
     2641 atgaatatac gttatcaaat atgaagtttg ctttgagtat tcttccgcac gatttgtcca
     2701 taaatgatta ttatcgtaaa gttaaggagt tacgggcaaa aaatcaaata acactacccg
     2761 taattctgaa aaatgagcgg caaattaatg tttttttaag aacggaagat attgatttaa
     2821 ttaatgtaat taatgaagaa acattattgc aacaacctga agagcgtttt gcatggttaa
     2881 ggtcaaagaa agataggttc tgataaaact ttcttgtcat cggctccgtt cgccgttatg
     2941 atcggtcgtc ttttaagcaa ctattgacac acacatgaag gcaaaagcga tattactcgc
     3001 ctctgtcctg ctcgtgggtt gccagagtac cggcaacgtt caacagcacg cacagagcct
     3061 ttctgcagct ggtcaagggg aagcagcaaa gtttacaagt caggcacgat ggatggacga
     3121 tgggacgtct atcgcgccag atggtgactt gtgggctttc attggcgacg agctaaagat
     3181 gggaattccg gaaaatgacc ggattcgcga acagaaacag aaatatttac gcaataagag
     3241 ctatctccac gatgtaactt tacgggcaga gccgtatatg tactggatag cagggcaagt
     3301 taaaaaacgt aacatgccta tggaactggt actactaccc atagtggaga gcgcttttga
     3361 tcctcacgca acgtctggcg ccaatgccgc aggcatctgg cagatcattc cgagcacggg
     3421 gcgcaattat ggtttgaaac agacccgcaa ttatgacgcg cgtcgcgatg ttgttgcttc
     3481 aacaactgcc gcgctgaaca tgatgcagcg tctgaacaag atgtttgacg gcgactggct
     3541 tctgaccgta gcggcttata acagcggcga aggtcgggtc atgaaggcaa ttaaaacgaa
     3601 caaagcgcgt gggaaatcca cggacttctg gtcgttaccg ttgccgcagg aaacgaagca
     3661 gtacgtgcct aaaatgctgg cattgagtga tattctcaaa aacagcaagc gttatggcgt
     3721 acgtctgcca acgaccgatg aaagccgtgc tctggcgcgt gtgcacctga gcagcccggt
     3781 tgaaatggcg aaggttgcag atatggcggg gatttccgtc agcaagctga agacattcaa
     3841 cgctggcgta aaaggctcca cgctgggcgc aagtggcccg cagtacgtga tggtgccaaa
     3901 gaagcatgca gatcaactgc gtgattctct cgcttcaggc gaaattgctg ctgtacagtc
     3961 gacgctggtt gccgacaata cgccgcttaa cagccgtgtt tacaccgtac gctctggcga
     4021 cacgctttca agcatcgctt cacgtctcgg cgtaagcacc aaagatttgc agcagtggaa
     4081 caaactgcgc ggatctaagc tgaagccagg ccaaagtttg acgattggtg caggcagtag
     4141 cgcacagcgg ttggcaaaca acagcgatag cattacgtat cgtgtgcgca aaggcgattc
     4201 gctttcaagc attgctaaac gccacggcgt gaacatcaaa gatgtgatgc gctggaacag
     4261 tgacactgcg aatctgcaac caggcgataa gctgacgttg tttgtgaaaa acaacaacat
     4321 gccagattcc tgacaaacca gataataaaa aaggcaccga ttcccccggt gcctttttta
     4381 tttatgccgc tttatgcgct tctaccatga tgatatcact ggtgaaagag ccatcagtct
     4441 gcaaggaaaa atacgttctc acctctgtcg atgcgctctg ttggtaaatg cgaatagcgt
     4501 ctactaacgc ttctggcgta cgcattctca cgacccatga agaaaattcc agcggtaact
     4561 tatctgtaat taaattatca actatcagat tggcttcatt gattaacgtc aaccacgcac
     4621 cgctggcgta gtttcgtacg tgagaggtat cgcgtaatgc ttctaccgcc tgtaaccaga
     4681 tgtcgcgcac tgggtgaccc ggagacatta catccatcac aatcagccta ccgccaggtt
     4741 tcaatatcct attcacttct cgcagtgctg caccaacatc atgccagtga tgggcagaat
     4801 aacggctgat aacaatatca aatgcgttat cggcaaatgg cagacttgcg gcatatccct
     4861 ggcgggtggt gatatttttc agttgccggg cttcggcagc ttgtgccaca acatccagca
     4921 tgtgggccga taagtcatac gccaccaccg cgctcacgtt ttgcgcagcg ccaaacgttg
     4981 gtctgccctg tggcagacct gacatacctt atttatcagg cttgccggga ctgaattcaa
     5041 cgagtagcgg attgtgatcg gaagcgcgcg taaccagtac agaagcttca ctgacgttca
     5101 gaccacggta gaaaacaaaa tcgagcgggc gaccaaacgc ccgacggcgc tgatcatcgg
     5161 taaaacgcac ctggcgcagc gacatttccc gcgcaaagcg atataacgcg ttcatccttc
     5221 tacggctcca ggcattgaaa tctcccgcca taatgaccgg gccgctgtgg tgagctatct
     5281 gatcaccaat aggaagtaac tgcttactat agacatccac gcccagactg aagttgacgg
     5341 cgtgtatatt aaccaccatc aacaggcggg tgtcaggtaa tggatagacc gtcaccagtg
     5401 ccgacttcgc cagacgcaaa atgggttctc gttcacgtaa cgggcagcaa tacactggat
     5461 gtgccgccga aagggtcatt acgccagaag gatgttgtgg cagcacgaaa gcgggtacct
     5521 gatcggcggc aagatagtta gcggtcgcaa actgtactaa ctctggcgtt gtctgcgctt
     5581 cctgcaataa caccaggtgt gcatctttgc cgtagttctt taataccgac aaccattcag
     5641 cgcgttgctg tttgtatatg ttccacacca ggatccgaat acgctcttcg gtacttaacg
     5701 gttccccagg tggtaatgcc tggccgatgc tcgcaaaaga ccccggcggt aagatccttt
     5761 ccgcaggttg tccggcaaca tagcgcatgg cataggtgtt ttttcgcact tttgactttg
     5821 aaacctctat tacgtcaacc cttccgcact cgggagggtt ctactattat agggatttta
     5881 gctcactctt tcaacgagca attgtatatt gttatgtaag caagtgctta caccggtcag
     5941 aattttgctc attatggaga acattaaatc gcatgacata tcacgcatcc tgatggttta
     6001 atgacaaata aattcaaaat atctcttcta ttttgcaaca ggagcaaaaa tgaaagccac
     6061 gtcggaagaa ctcgccattt ttgtttcggt cgtcgaaagc ggcagcttta gccgggcagc
     6121 ggaacaatta gggcaagcaa actcagcggt aagccgggcg gtgaaaaagc tggagatgaa
     6181 acttggcgtt agcctgctta atcggaccac gcgacaactt agcctgacgg aagaaggcga
     6241 gcgttatttt cgtcgcgtac agtcaatttt gcaggagatg gcagcggcag aatcagaaat
     6301 tatggagacg cgtaatacac cgcgtggact gttacggatc gatgccgcaa ctccagtggt
     6361 gctgcacttt ctgatgccgt taattaagcc tttccgtgaa cgctatccgg aagtcacttt
     6421 gtcactagtc tcctccgaaa cgattattaa tttgatcgaa agaaaagtgg atgtcgcgat
     6481 acgcgctggt acgttaacgg attccagctt acgtgccagg ccgttattta acagttatcg
     6541 aaaaattatc gcctcccccg attatatttc ccgctacggg aagccagaaa cgatcgacga
     6601 tttaaagcaa catgtttgcc tgggattcac tgaacccgct tccctcaata cctggccgat
     6661 agcctgtagt gatggacaat tacatgaggt gaaatacggt ttgtcatcca atagtgggga
     6721 aacactgaaa cagctttgcc tgagtggcaa cgggattgcg tgtttgtccg actacatgat
     6781 cgacagagaa atcgctcgcg gagaattggt ggagttaatg gcagataaag tgttgccagt
     6841 ggaaatgcca ttcagtgccg tctattacag cgaccgtgcg gtaagtacgc gcatccgggc
     6901 ttttatcgat ttccttagcg agcatgtaaa aacagctccc ggaggagctg tcagagaggc
     6961 ttaatcccat tcaggagcca gaccttccgg gctaaccagg cggtcgttgc aatccagtgc
     7021 ggcgatcgct tttttatctt cggcatcaag ctgtaaattt tgtgccttaa gattactttc
     7081 caggttttta cgtttagtag aagaaggaat tactgagtaa ccttccccca tagcccacgc
     7141 cagaatcact tgtgccggag tcgcattgtg tttagctgcg atacgagcaa taacctcatc
     7201 tttcagggcc ttaccatacg ccagcgtcat ataggaagta atatgtatgc cgtgctgttt
     7261 agcccaggca accactttac ggttttgcag ataaggagag agttcaatct ggttagtagc
     7321 gatgttttca gcgccaacag cagcaatcgc cttttccatc aatgggatcg tgaagttgga
     7381 aataccgatc tcacgcgtca gcccttgttt tttggcttcc agcagcgcct gcataacccc
     7441 ttcaacagag acttcatcgt ttggtgacgg ccagtggatt agcgtcagat caacataatc
     7501 ggtacgcaat ttttgcaggc tctctttcag acttgggatc aatttgtctt tgctgagatt
     7561 ttcaatccag attttagtgg tgatgtagag ttcatgacgt ggcacgccac tttctgcaat
     7621 cgcctgacct actgcggctt cgttatcata gatttgtgcg gtatcaattg cgcgataacc
     7681 aagttcaagc gccgttttca cagatgaaat aacaacgtag tctttcaacc taaaccaaat
     7741 gcagggatag ccataatatg cctcttttaa tgctattttc gttaactgag gtgattatgg
     7801 gcttcagact gttgaggaaa aaagcgtaaa atgcagagga tttttgcgat tctggcaata
     7861 atagatatac agacgaaaaa aaacctcgag cgttaactcg aggcttctta ata
//
LOCUS       DAJTSL010000176         7818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTSL010000176 DAJTSL010000000
VERSION     DAJTSL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.176"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            268..411
                     /locus_tag="OO433_003546"
     CDS             268..411
                     /locus_tag="OO433_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6051887.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            424..633
                     /gene="cspE"
                     /locus_tag="OO433_003547"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             424..633
                     /gene="cspE"
                     /locus_tag="OO433_003547"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6051888.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            799..1608
                     /gene="rlmA"
                     /locus_tag="OO433_003548"
     CDS             799..1608
                     /gene="rlmA"
                     /locus_tag="OO433_003548"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6051889.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="OO433_003549"
     CDS             complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="OO433_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6051890.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2599..3057)
                     /locus_tag="OO433_003550"
     CDS             complement(2599..3057)
                     /locus_tag="OO433_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6051891.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDNLERIYKLIVLTQ"
     gene            complement(3112..3963)
                     /gene="manZ"
                     /locus_tag="OO433_003551"
     CDS             complement(3112..3963)
                     /gene="manZ"
                     /locus_tag="OO433_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6051892.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(3976..4776)
                     /gene="manY"
                     /locus_tag="OO433_003552"
     CDS             complement(3976..4776)
                     /gene="manY"
                     /locus_tag="OO433_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6051893.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4839..5810)
                     /gene="manX"
                     /locus_tag="OO433_003553"
     CDS             complement(4839..5810)
                     /gene="manX"
                     /locus_tag="OO433_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6051894.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            6273..>7818
                     /gene="yoaE"
                     /locus_tag="OO433_003554"
     CDS             6273..>7818
                     /gene="yoaE"
                     /locus_tag="OO433_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6051895.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDVDNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDED"
BASE COUNT         1987 a         1913 c         1940 g         1978 t
ORIGIN      
        1 gcactatgta ataacttctc ccactggcct ggaacaactg aacttattga actatgttag
       61 aaaatacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg
      121 cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc
      181 tgatcgagac atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt
      241 cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt
      301 aaacacgttg atgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt
      361 aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt
      421 caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc
      481 attactccgg ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat
      541 ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa
      601 ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat
      661 acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt
      721 cgctcctggc gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag
      781 tatcctgccg gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt
      841 gaaaaaaaca gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat
      901 gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg
      961 atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt
     1021 gtcgcccaac tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt
     1081 ggtgaagggt attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt
     1141 ctggatgttt cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt
     1201 tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt
     1261 atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc
     1321 attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa
     1381 gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg
     1441 gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg
     1501 ccgtttgcct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc
     1561 gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc
     1621 gtccagagga tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttccgctttt
     1681 ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag
     1741 gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca
     1801 acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa
     1861 ccgtgtcgac ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc
     1921 attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca
     1981 aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca
     2041 ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct
     2101 ttaccgattg atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca
     2161 gtgatattca tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca
     2221 aacatgcata catgaacaac aagccgtgat ggttttcgcg ttcatgcagg catagcgatt
     2281 gatgtgcata tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa
     2341 cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc
     2401 gcaaccttaa catattttga gaatataaaa caactgttgg aatatattat ttcattgctt
     2461 tattgataac gaattcattt agatttatat atcaatgaaa cgttaaaata aataacaaat
     2521 aagggaagag gtaatatagc ctaagctatg tctgattatt aaaaatacac taaacatagc
     2581 tttggctaaa ttcataactt attgagttaa aacgataagc ttataaattc tttccagatt
     2641 gtcgatattt cgaacgcgga ttaacagccg acgctgttct aattgcatca ccaacacgcc
     2701 atcttccgac aagttcatcg ctttgattcg gctatattca atccagacat tggcgaagaa
     2761 aaaacctttt tgtttaaaga tgatcttcgg aacgcggatc cagaatatat aaaaacccat
     2821 cagagccagt gcgcttaata accatgtcgt tattaatgca ccatgattcg tgacgttgtt
     2881 atagataaga atgacaatca atccgacgaa gataacgcta tcgatgcgac cacgccggag
     2941 caaaggaatt gccagcaggg tggggccgtt acggcggggc atgatgaact gatcgtagat
     3001 cgcgaaggcc aggagtgcgg cgatgaaaag aatcagtacc aggtccgtga ttgtcattaa
     3061 tcctccagat aaaaaaacgg ggccaaaagg ccccggtagt gtacaacagt cttacagtcc
     3121 cagcaggccg caagcgtaac cagcgatacc gatgacgaag aagccaacga tgatccacag
     3181 cgggttaact tttttgcgca gtagccacat acaagcaaag gtcagcagca gtggtaccag
     3241 gcctggcatt aactggtcca gaatagtctg gacagtagta acgtgttctt tgcccgtctg
     3301 gtcagtaatg cgagagacaa ccagcgggat gttgacatgt gtccacttgt taaccaatgc
     3361 ccccatgaca aacaggccga ggatagacgc cccttccgtc agtttttgca ggaagccacc
     3421 acccatatct ttaacgatat cgataccttt ggagtaacca tacgctacgc cgtagtaacg
     3481 ggttgccaga cgcaccaggt taaacaggat gaagaacagc agcggaccta acaggctgcc
     3541 gctcatcgcg ataccggcac ccagtgctgc aaataccgga cgtacggttc cccagaagat
     3601 cgggtcgcct acaccagcca gcggccccat caaaccgact ttgataccgt tgatagcacc
     3661 gtcgtcgatc tctgcgccat tagcacgctg ttcttccagc gccagggtta cgccgagaat
     3721 cggcgcagcc acgaacggct gggtgttaaa gaactccagg tgacggcgaa tagcttgttt
     3781 acgagcttcg ttgttctcag ggtagaggcg acgaattgcc ggtaccatag agaagcagaa
     3841 acccagtgcc tgcatacgtt cgaagttcca tgaaccctgg aagaggttag aacgcaggaa
     3901 gacgccacga atatcacttt gagtgagttt tttctcggtg gtagtttgag ttgtatcaac
     3961 catttcgctc acctgttagt ccagttcgtt atcgagatcg ttgttaccag cagcctgagc
     4021 aggcgcaccg gctacgcggt tgtatttcgg gctaagttgg atgtagagca ctgccataac
     4081 agtaccgatc acacccagag caaccaggtt aaagttggtg aatgctgcgg ttacgaagcc
     4141 gaggtagaag aacggcatca ggtagccagc acgcatcatg ttgataacca tcgcataacc
     4201 aaccaccacg atcatgccac cggcgatatt cagaccattg gtcaccactt ccggaatcgc
     4261 attcagcatg ttctgtactt cgctggtacc aacagacagc gcaacgatga cggccggaat
     4321 agccacacgc attgcttgca ggaacagaga agaaacgtgg atccaggaaa tcgctgtcag
     4381 gttgccgtta tcagcagcct tatccgcagc gtgctggaaa gcaacggtaa tagtacgaac
     4441 gatgatggtc agtacctggc ccgcagcggc cagagggatt gccagtgcga tacctgcacc
     4501 aatgctctga tgacctgcga taaccagaat ggtagaaatg atagaagcca gagcggcgtc
     4561 aggcgcaact gcagcaccga tgttcatcca gcccagcgcg atcatttcca gcgtaccacc
     4621 gataataata ccggttttca tatccccaag aacgataccc accagggtac acgcgattag
     4681 cggacggtga aactgaaatt catcgaggat tgatcccata cctgcgatac aggctacgat
     4741 aaataccagc acaatttgaa gagtggtaat ctccattgta cttctcctgt ttacgtaaga
     4801 cttaagtgtg aaaactgagt gataatcaac acaatacgtt acttatcgat tttgctgatc
     4861 agatccatca ttttcagttt cggatcggtg gaaaccttac ggacttccag ctcaataccg
     4921 cgcgcattca gtttcttgaa cgcctcgata tctttttcat caaccgaaac cgcgttattc
     4981 acctgggttt taccctgacg gaatgccata ccaccgacgt taacagaggt gattttcacg
     5041 ccgccttcaa cgagacgctc tacatctgtt gggttggtaa ataacagcat tacgcgttcg
     5101 ccagcatatt tcgggttgtt gtagacgcga atcattttgg caacatcaac tacgtgtgct
     5161 gttacgcccg gaggtgcaac ctgggtgagc agtgtcttac gaacggtatc cgcagccact
     5221 tcatcactaa caacaataat acgggagaca ttggtttctt tggtccagcg ggtggcgacc
     5281 tgaccgtgaa tcagacggtc gtcgatacgc gcaaggccaa taaccatgta gtcgtttggc
     5341 cccattggtt ttgccggagt tggagccgct tttggtgctg cggcagcggg tgctggcgcg
     5401 gctttttcaa ccggtttggc tttcagtgct ttcacacctt cacggcctgt ttctactgcc
     5461 agcgcaacca gttcatcaaa gcttgggtca tcatcacgag ccattaacgt ttccacgagc
     5521 attggaatgt taacgcctgc aatgacttca taatgctctt tgtcgacgac aatgcggctg
     5581 gcagcattga acgggctgcc tccccatgta tcaacgagaa acagcacgcc tttagtggtg
     5641 tcgagttttg ccaactgagc gttgtacttt tcaatcagcg tttcggcatt ttcacctgga
     5701 acgaaatcga tccagccgac gttttcctgc tcgcctaaca gcatttctgc cgttttaagc
     5761 aactgctctg cagcccaacc atgtgtgcct ataacaatag caatggtcac ttgctacctc
     5821 ctttattatc gttaacacct caacgtgcca gatgtatttt tgaatcgtcg tccacaatcg
     5881 aatcgattca gataagggct acaaccaaaa acccctgcgt ttcgcgattt attttagata
     5941 tcgaaaaaat tattttatgt gatgaagatc cgtaatttaa ctttcgatta gcagaaattt
     6001 cgaaaggtaa aatatccttg tcacattcgt ttgcaaagga aggtaaatct ttgccaaatc
     6061 agaggcgtct ctgatatgtt taactcccgt ttaacaacca tggagtatag ggcagtagcc
     6121 cgcagtatgg atcgtcaccg acgtcatttc agcatcaggc cttttaacgc ctgcctttct
     6181 ggcactctat gccgcacctt tcgtttgcat tttgtcgtta cgcctgcatt atttctggcg
     6241 tcgaatagct attccttaag caggagcttg tcatggaatt cttaatggac ccctcaattt
     6301 gggcggggct actcacgctt gttgttctcg aaattgtgct gggtatcgat aacctggtct
     6361 tcatcgccat tcttgctgac aaactgccgc caaaacaacg cgataaagcg cgtttgctgg
     6421 ggttatcact ggcgctgatt atgcgtctgg ggctgctgtc gctgatttca tggatggtca
     6481 cgctgaccaa accgctattt accgtcatgg atttctcctt ctccggacgc gacctgatta
     6541 tgttgttcgg ggggatattc ttgctgttca aagcaacaac cgaactgcat gaacggctgg
     6601 aaaaccgcga tcatgattcc ggccacggta aaggctacgc cagtttctgg gtggtcgtca
     6661 cacagattgt catccttgac gccgtcttct cgttggatgc ggtaattact gcagtaggga
     6721 tggttaacca tctgccggtg atgatggcgg cggtagtgat tgcgatggcg gttatgttgc
     6781 tggcatccaa accgctgacg cgattcgtta accagcaccc cacggtggtg gtgctctgtc
     6841 tgagcttcct gttaatgatt ggtcttagtc tggtggcaga aggtttcggt ttccacattc
     6901 cgaaaggtta cctgtatgcc gcgattggct tctcgatcat catcgaagtg tttaaccaga
     6961 ttgcgcgtcg caactttatt cgccaccagt cgactttgcc gctgcgagcg cgtactgccg
     7021 atgccatcct gcgtttgatg ggcgggaaac gtcaggccaa tgttcagcac gatgtcgata
     7081 acccgatgcc gatcccggaa ggtgcatttg ccgaagaaga acgttacatg attaacggcg
     7141 tactgacgct ggcgtcgcgt tctctgcgcg ggatcatgac gccgcgcggt gaaataagct
     7201 gggttgacgc taatctcggg gtcgatgaaa tccgcgagca actgctctct tcaccgcaca
     7261 gtctgttccc ggtatgtcgc ggtgaactgg atgaaatcat cggtattgta cgtgctaaag
     7321 aactgctggt ggcgctggaa gagggcgttg atgtggcggc gattgcttcg gcgtctccgg
     7381 cgattatcgt cccggaaacc ctcgatccga tcaacctgtt gggcgtgctg cgtcgtgctc
     7441 gcgggagctt tgttatcgtg accaacgagt ttggtgtggt acaaggtctg gtcacgccgc
     7501 tggatgtgct ggaagccatt gcgggtgaat tcccggacgc tgacgaaacg ccggaaatca
     7561 ttactgatgg tgacggctgg ctggtaaaag gcggtacaga tttgcatgcc ttgcagcagg
     7621 cgcttgatgt tgagcacctt gccgatgacg atgatatcgc gacggtcgcg ggcctcgtga
     7681 tctcggcaaa tggtcacatt ccccgtgtgg gcgatgtgat tgatgtaggg ccactgcata
     7741 tcaccatcat tgaagccaat gattatcgtg ttgatctggt tcgcattgtt aaagagcaac
     7801 cggcgcacga tgaagatg
//
LOCUS       DAJTSL010000177         7758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTSL010000177 DAJTSL010000000
VERSION     DAJTSL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.177"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..590
                     /locus_tag="OO433_003555"
     CDS             <1..590
                     /locus_tag="OO433_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HCR6051896.1"
                     /translation="GDNKDATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHF
                     QAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQ
                     FEKLSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQL
                     ISFAKSFDPTSKEEIKILTDTSKLIYDLFGVKSER"
     gene            953..1990
                     /gene="dusA"
                     /locus_tag="OO433_003556"
     CDS             953..1990
                     /gene="dusA"
                     /locus_tag="OO433_003556"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6051897.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2124..2366
                     /gene="pspG"
                     /locus_tag="OO433_003557"
     CDS             2124..2366
                     /gene="pspG"
                     /locus_tag="OO433_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6051898.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2531..3514)
                     /locus_tag="OO433_003558"
     CDS             complement(2531..3514)
                     /locus_tag="OO433_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6051899.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGSGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3597..5012
                     /gene="dnaB"
                     /locus_tag="OO433_003559"
     CDS             3597..5012
                     /gene="dnaB"
                     /locus_tag="OO433_003559"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6051900.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5065..6144
                     /gene="alr"
                     /locus_tag="OO433_003560"
     CDS             5065..6144
                     /gene="alr"
                     /locus_tag="OO433_003560"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6051901.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAGAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6397..7590
                     /gene="tyrB"
                     /locus_tag="OO433_003561"
     CDS             6397..7590
                     /gene="tyrB"
                     /locus_tag="OO433_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6051902.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNLGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1880 a         1855 c         2028 g         1995 t
ORIGIN      
        1 ctggggataa taaagatgcg actcccattg aacaatgtac ttttgatgta gaaactaaga
       61 atggccttcc ctccacggtt cagctatttt atgaggaatc taaggataat gaacctttag
      121 cgaatataca ttttcaagca ataggttctg gttttttaac gtttgttaat gcctgccagg
      181 aacatgatga caacagctta aaattatttg cttcgctgtt aatttcactt tcatattcta
      241 gtgcctacgc agatttatca gaaacagtgt atattaatga aaataatgag agctacctga
      301 aagctcagtt tgaaaaatta tctcaacgtg atatgaagaa gtacctggga gagatgaagc
      361 gtctggctga tgggggagaa atgaattttg atggctatct ggataagatg tcacatctgg
      421 tgaatgaagg gacgctcgat cctgatattt taagcaaaat gcgagatgct gcaccacaat
      481 taattagctt cgcgaagtcg tttgacccaa cctcaaagga agagattaaa atacttacag
      541 acacttctaa attaatttat gatttgttcg gggttaaatc ggagagataa tatgtgaagt
      601 tcttcgatag tatggaaggc attatataaa aggacccaat atttattggg tccttttttc
      661 tctatcaatg ctattagcag ggagatatat caccaaagtt taatgtgtga ttttttattt
      721 atcgtcgaac ctggattgtt tatcattggc cttaacaaag ttaacggcta ataagattat
      781 ttccatcact tcgtgagagc ttcatgcctt gagaggatct caattttctt ttgcaatgag
      841 acaggcgctt cctgttgtta tggtatagta ccccgctatt gagcctcctg aatagtgatg
      901 ctgaataaca taaccccatg atatatcgat aaaataatct ctacatttga aaatgcacgg
      961 taattctgaa atgcaaaaaa tcaaccaaac cagcgcaatg cctgaaaaaa ctgacgttca
     1021 ctggagtggt cggtttagcg ttgcaccaat gctcgactgg acggacagac attgccgcta
     1081 tttcttgcgt ctgctttccc gcaatacgtt gctgtatacc gaaatggtga ccacaggggc
     1141 gattattcac ggtaaaggtg attacctggc gtacagtgaa gaagaacatc cggtagcgtt
     1201 gcaactcggc ggtagcgatc cggcggcgct ggcacagtgt gcgaagctgg cagaagcgcg
     1261 tggatatgat gagatcaacc tgaatgtcgg ctgcccgtct gaccgggtgc agaacggcat
     1321 gtttggtgcg tgtctgatgg gtaatgcgca gctggttgcc gattgcgtga aagcgatgcg
     1381 cgatgtggtg tcgattccgg tgacggtgaa aacgcgtatt ggcatcgatg accaggacag
     1441 ctatgaattt ctctgcgatt tcatcaacac cgtttccggc aaaggcgagt gtgggatgtt
     1501 catcatccat gcacgtaaag cctggctttc ggggttaagc ccgaaagaaa accgtgaaat
     1561 cccgccgctc gattatccgc gtgtgtatca actgaagcgt gactttccgc atctgacaat
     1621 gtcgattaac ggtggtatca agtcgctgga agaggccaaa gcacacctgc aacatatgga
     1681 tggcgtgatg gtcgggcgcg aggcgtatca gaatccgggt attctggcgg cggtagaccg
     1741 ggagatcttt ggttcctcgg ataccgatgc ctatccggtg gcggtagtgc gcgccatgta
     1801 tccgtacatt gagcgtgaac tcagccaggg gacgtatctc ggccatatta cccggcatat
     1861 gttgggcttg ttccagggta ttcctggcgc gcggcagtgg cggcgttatt taagtgaaaa
     1921 tgcccataaa gcgggtgcag acattaatgt gctggaacac gcgctcaaac tggtggcgga
     1981 taagcgttaa cttttcacca aaaagtagtc aaattcacca cgccctgcgc accgtcgcgg
     2041 ggcgttttgc tgttaaatca atagattatt tttggcatga ttcttgtaat gccagcaaga
     2101 gatttcatat ttgggagagc atcatgctgg aactactttt tgtgattggc ttttttgtca
     2161 tgctgatggt caccggcgtt tcgttgctgg gcattatcgc cgcgctggtt gtggcgacgg
     2221 ccattatgtt cctcggcggt atgctggcat tgatgattaa gttgctgccg tggttactac
     2281 tggcgattgc ggtggtgtgg gttattaagg cgattaaagc accaaaagtg cctaaatatc
     2341 agcgttatga ccgctggcgt tactaaggga ttgtgcggat gatcacaacc taaggtttta
     2401 tccttagaac aaaataggaa ttgataatca aatctgtcac tattgcgcct ctaacagatt
     2461 catcgtgctg taccctacat acagccgact ataaaaagaa agggcttccc aggtggaagc
     2521 cctatttctt ttatggaatc agcaggctgg aaccttgcgt cgcccggctt tccagaatct
     2581 catgcgcacg ctgcgcatcc ttcagcggat atttctgctg ctcggcgaca tcgaccttaa
     2641 tcacaccgct ggcaatcaaa gagaacagtt cattactggc ctcggttaat tcctcccgtg
     2701 tggtgatata gccttgcagg gaagggcgtg tcacatacaa cgagcctttt tgattgagaa
     2761 tgcctaagtt cacaccggta accgcacctg atgagttgcc aaaactgacc attaagccgc
     2821 ggcgttgcag gcaatccagc gaccgttccc aggtgtctct gcccacggaa tcgtacacta
     2881 cgcgcacttt cttaccgccg gtgatctctt tcaatcgctc gaccagattc tcttcacgat
     2941 agttaataac ctgccacgcg ccggctttta gcgcgctctg cgctttttgc gcggttccta
     3001 cggtgccgat aagtttcgcg cccagagctt ttgcccactg gcaggcaatc aagccaacgc
     3061 cgccagccgc tgcgtggaac aggaactgtt catcgggttt aatttcatag gttttgcgca
     3121 gcagataata aaccgttaag cctttcagga aagatgctgc agcttgctca aaagaaatag
     3181 ccgcaggcag aatcgccgct ttatccgcat taatgttatg cacagagctg taagcgccta
     3241 acgccgactg cgcatagact acacgatcgc ctgccttaat atgctttaca ccactgccga
     3301 ctttactcac gatgcctgcc gcttcggtac ctgatccgct gggtagtgat ggcggcgggt
     3361 aaaggccgct gcggatatac gtgtcgataa aattgatgcc gatggcttta ttttcgacct
     3421 ggatttcatt ctctgccgga tcggcaggag tgaactctac ggcttgaagt acttccgggc
     3481 caccgtgctt gtgaaattca attcgtgttg ccatgtgtcc tccagaacgt atcgtcaggg
     3541 tctgcttcat atggtaaagt ttcgacccat tctttatctc ggtaactcca ttcactatgg
     3601 caggaaataa acccttcaac aaacagcagg ctgaaccccg cgaacgcgat ccacaagttg
     3661 ccgggctgaa agtgcctccg cactcgatcg aagcggagca gtcggtgttg ggcggtttaa
     3721 tgctggataa cgaacgctgg gacgatgtag ccgagcgtgt ggtggcagac gatttttaca
     3781 cccgcccaca ccgccatatc tttactgaaa tggcgcgttt gcaggaaagc ggtagtccta
     3841 tcgatctgat tacccttgcg gaatcgctgg aacgccaggg gcaactcgat agcgtcggtg
     3901 gttttgctta tctggcagag ctgtcaaaaa atacgccaag tgcggcgaac atcagtgctt
     3961 atgctgacat cgtgcgtgaa cgtgccgttg tccgtgagat gatctcggtt gcgaatgaga
     4021 ttgctgaagc cggttttgat ccgcaggggc gtaccagcga agatctgctg gaccttgctg
     4081 aatcccgcgt ctttaaaatt gccgaaagtc gtgcaaacaa agacgaaggg ccgaagaaca
     4141 tcgccgatgt gctcgacgca accgtggcgc gtattgagca gttgtttcag cagccacacg
     4201 atggcgttac cggagtaaac accggttatg acgatctcaa caaaaaaacc gctggcttgc
     4261 agccgtcgga tttgatcatc gtcgccgcgc gtccgtcgat gggtaaaaca acatttgcga
     4321 tgaacctcgt cgaaaacgcg gcgatgttgc aggataaacc agtacttatc ttctcgctgg
     4381 agatgccttc agaacagatt atgatgcgtt ctctggcgtc gctgtcgcgc gttgaccaga
     4441 ctaaaatccg taccgggcag ctcgatgatg aagactgggc gcgcatttcc ggcaccatgg
     4501 gtattttgct cgaaaaacgc aatatctata tcgatgactc ctccggcctg acgccaacgg
     4561 aagtgcgttc ccgcgcacga cgtattgccc gtgaacacgg cggcatcggg cttatcatga
     4621 tcgactacct gcaactgatg cgcgtaccgg cgctttccga taaccgtacg ctggaaattg
     4681 cagaaatctc tcgctcgctg aaagcactgg cgaaagaact gaacgtgccg gtggtggcgc
     4741 tgtcccagtt gaaccgttct ctggaacaac gtgccgacaa acgcccggtc aactccgacc
     4801 tgcgtgaatc tggctctatc gagcaggatg cggacttgat catgtttatc tatcgtgatg
     4861 aggtgtatca cgaaaacagt gatttaaaag gcatcgcgga aattattatc ggtaaacaac
     4921 gtaacggccc aatcgggacg gtacgcctga cctttaacgg tcaatggtcg cgcttcgaca
     4981 actatgcggg gccgcagtac gacgacgaat aataattatt ttatgaatta ggcaattaaa
     5041 gcaaacactt atcaaggaac acaaatgcaa gcggcaactg ttgtgattaa ccgccgcgct
     5101 ctgcgacaca acctgcaacg tcttcgtgaa ctggcccctg ccagtaaaat ggttgcggtg
     5161 gtgaaagcga acgcttatgg tcacggtctt cttgagaccg cgcgaacgct ccccgatgct
     5221 gacgcctttg gcgtagcccg tctcgaagaa gctctgcgac tgcgtgcggg gggaatcacc
     5281 aaacctgtac tgttactcga aggctttttt gatgccagag atctgccgac gatttctgcg
     5341 caacattttc ataccgccgt gcataacgaa gaacagctgg ctgcgctgga agaggctagc
     5401 ctggacgagc cggttaccgt ctggatgaaa ctcgataccg gtatgcaccg tctgggcgta
     5461 aggccggaac aggctggggc gttttatcat cgcctgacac agtgcaaaaa cgttcgtcag
     5521 ccggtgaata tcgtcagcca ttttgcgcgc gcggatgaac caaaatgtgg cgcaaccgag
     5581 aaacaactcg ctatctttaa taccttttgc gaaggcaaac ctggtcaacg ttccattgcc
     5641 gcgtcgggtg gcattctgct gtggccacaa tcgcattttg actgggtgcg cccgggcatc
     5701 attctttatg gcgtctcgcc gctggaagat cgctccaccg gtgccgattt tggctgtcag
     5761 ccagtgatgt cactaacctc cagcctgatt gccgtgcgtg agcataaagc cggagagcct
     5821 gttggttatg gtggaacctg ggtaagcgaa cgtgataccc gtcttggcgt agtcgcgatg
     5881 ggctatggcg atggttatcc gcgcgccgct ccatccggta cgccagtgct ggtgaacggt
     5941 cgcgaagtac cgattgtcgg gcgcgtggcg atggatatga tctgcgtaga cttaggtcca
     6001 caggcgcagg acaaagccgg ggatccggtc attttatggg gcgaaggttt gcccgtagaa
     6061 cgtatcgctg aaatgacaaa agtgagcgct tacgaactta ttacgcgcct gacttcaagg
     6121 gtcgcgatga aatacgtgga ttaatcgttc tgtaatattt gattgtctgt gccggatgcg
     6181 gcgtgaatgc cttatccggc caataaaatc ctaaaaattc aataagttga tgttctttca
     6241 tgctcttata aaggtcgtgc ctctggcgga tgttcgtttg tcatgagtct cactctgttg
     6301 ctaattgccg ttcgctcctg aacatccact cgatcttcgc cttcttccgg tttattgtgt
     6361 tttaaccacc tgcccgtaaa cctggagaac catcgcgtgt ttcaaaaagt tgacgcctac
     6421 gctggcgacc cgattcttac gcttatggag cgttttaaag aagaccctcg cagcgacaaa
     6481 gtgaatttaa gtatcggtct gtactacaac gaagacggaa ttattccaca actgaaagcc
     6541 gtggcggagg cggaagcgcg cctgaatgcg cagccccatg gcgcttcgct ttatttaccg
     6601 atggaagggc ttaacagcta tcgccatgcc attgcgccgc tgctgtttgg tgcggaccat
     6661 ccggtactgc aacaacagcg cgtagcaacc attcaaaccc ttggcggctc aggggcattg
     6721 aaagtgggcg cggatttcct gaaacgctac ttcccggaat caggcgtctg ggtcagcgat
     6781 cctacctggg aaaaccacgt agcaatattc gccggggctg gattcgaagt gagtacttac
     6841 ccctggtatg acgaagcgac taacagcgtg cgctttaatg acctgttggc gacgctgaaa
     6901 acattacctg cccgcagtat tgtgttgctg catccatgtt gccacaatcc aacaggtgcc
     6961 gatctcacta atgatcagtg ggatgcggtg attgaaattc tcaaagcccg cgagcttatc
     7021 ccattcctcg atattgccta tcaaggattt agtgccggta tggaagagga tgcctacgct
     7081 attcgcgcca ttgccagcgc tggattaccc gctctggtga gcaattcgtt ctcgaaaatt
     7141 ttctcccttt acggcgagcg cgtcggcgga ctttctgttc tgtgtgaaga tgccgaagcc
     7201 gctggccgcg tactggggca attgaaagca acagttcgcc gcaactactc cagcccgccg
     7261 aatttgggtg cgcaggtggt ggctgcagtg ctgaatgacg aggcattgaa agccagctgg
     7321 ctggcggaag tagaagagat gcgtactcgc attctggcaa tgcgtcagga attggtgaag
     7381 gtattaagca cagagatgcc agaacgcaat ttcgattatc tgcttaatca gcgcggcatg
     7441 ttcagttata ccggtttaag tgccgctcag gttgaccgac tacgtgaaga atttggtgtc
     7501 tatctcatcg ccagcggtcg catgtgtgtc gccgggttaa atacggcaaa tgtacaacgt
     7561 gtggcaaagg cgtttgctgc ggtgatgtaa tgcaggaaag caggctggag ttacccagcc
     7621 tgcagtgaaa ttaaactgtc gtcgctttca ctatttcttt gtagatgatt tttttgatgc
     7681 cgttgttcta cgtgagagat aataaaacgt tgtcagttct tttattgtta agtttatccc
     7741 aattaactgg aattcctt
//
LOCUS       DAJTSL010000178         7640 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTSL010000178 DAJTSL010000000
VERSION     DAJTSL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.178"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            565..3978
                     /locus_tag="OO433_003562"
     CDS             565..3978
                     /locus_tag="OO433_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6051903.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIKAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEELKNTDQVTQEHPVKEQPTTSGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPVIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNRVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAIRFDMDILAAQTARYTNPEELDVLWPIIVEKVERLPPHLAQVQQQGQRINQLRN
                     LSWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIAQIARLQKIVEELGVVTDAATV"
     gene            4042..5676
                     /locus_tag="OO433_003563"
     CDS             4042..5676
                     /locus_tag="OO433_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6051904.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGKKLASLLKQQYPEGINAGVDFKDRTQAAFVVPKADIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            5685..6755
                     /locus_tag="OO433_003564"
     CDS             5685..6755
                     /locus_tag="OO433_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6051905.1"
                     /translation="MVKLGDVINVHYGKALKADQRVSNGSVHVFGSSGIVGNHDKTLC
                     SYPTIIIGRKGSVGAITWAPSGGWIIDTAYYVEIKDNNKLDLRYLFYILSGIDLTKKT
                     ITTSIPGLNRDDLYDTFIKLPPFEEQKRIVDLLDKAEGIRQKREQSIKLADDFLRATF
                     ATMYGNPITNPKKWPVHLMGEIIEFKGGNQPPKSDFIFEPKQGYIRLVQIRDFKSDKY
                     ATYIPQEKAKRIFEVDDVMIARYGPPVFQILRGLSGSYNVALMKASPKENIRKGFIFY
                     LLQLPEYHDVVVKNSERTAGQTGVNLELLNKFNVPLPPIYYQDEILDRLARIEKFKEK
                     IEISLNHLEIQFLSLQKRLIGF"
     gene            complement(6880..7596)
                     /locus_tag="OO433_003565"
     CDS             complement(6880..7596)
                     /locus_tag="OO433_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051906.1"
                     /translation="MLSETKKRIPAHLFRMIKDELYELVLTDDPKGKLYVRDSEKIEE
                     GVSTTEFVVGYGAISMVKKSESMATKGLVGLERVDLIREVVFENGYYDWGCVVNDVLP
                     NICKGNARIPIFHFLNHAKLINPDGSIINETELSGGVLSRLNITPISFQSQGWDKRRS
                     ETVPEVKVGVNELYLAYDFSFFLRMMPYMEPGLIKRDMGELLKILKKHIDEAMNTQVL
                     SSNFCKLVCIYDYIKNSHRY"
BASE COUNT         2221 a         1739 c         1792 g         1888 t
ORIGIN      
        1 gtcctgttct cccaaatatc atggtattga tacgggagat tatgccgtac aggtacgaca
       61 cgttatgtcg tgccaaaggg gagtggatag aaaaaatggc gaagatgcga aaagttgatt
      121 aatgcattga aaaaaatgaa ttaataatga acttgggtaa aagcgccatc aaaatacgac
      181 acgacctgtc gtattttgtg ttagccggga aaagatatct gctacttact aaaatgattt
      241 agtgctgaaa atatttataa gccaataaat agacaggtat tacggagtaa cgcccaggct
      301 attttaacga gcctgctgtt ttattggctc aaaaaacata tcccatagat gatcagccat
      361 acccgcgtta gtgagcaagg tatcccttga gtagccaggg ctgaagacgc ccattgatgg
      421 gcgaaaacca aggttctggc cgttaaagcg gctatgtcgt taaacacatc accaccatat
      481 gccatccggc tcacttcgtc ggccaaatta gtgtgatatt ctttccagaa tttctgttgt
      541 atccacaaca taaggcgatg attgatggag cagtctctca attttgaaat gttgcgcagc
      601 cagtggccgg aactggcaga actcgcatgt atggcagagc gctatgttca ctccgatccg
      661 gaaagctgcc tggttaagct gcgcaactac accgaattga tggtgcgctg gttgtatcgt
      721 caggagcggt tgccggaagg tattaaggct aatctttacg atttaatgaa cgctgatgtc
      781 tttaccagca tgatgccgga agccatcatc atgaaaatgg atgcgctgcg tatccatggc
      841 aaccgtgccg cgcacggcgg acgtatcaaa gctaaagata cttactggct gctcaaagaa
      901 gcgtatttgt tgggaatttg gctgtatgtt cgctacgccc acggtaatgt tgatgactgc
      961 cccaaattta cactccctcc attaacacaa tcttcaggtc gtgcagatga aaaacgtctg
     1021 gaagatgcaa tcaaggctca ggatgaaagc cgtgagcgcg aactggcgct acaacgcgca
     1081 ctacagcaag aacaggaaaa ggccgaacac ctcactcaac gcctgaatga agccagagcg
     1141 cgtaaccagc atgttgccga tattctctct attgatgaag cggaaacccg ccgtcgcctg
     1201 attgactctc gcttacttgc agccgactgg aatgtagggg aagaacttaa aaacacggat
     1261 caagtcacac aagaacatcc agtcaaagag caacccacca cctctggtga cggttatgca
     1321 gactatgtct tgtgggatga ggcacacaaa ccgttggcgg tggtggaagc aaaaaaaacc
     1381 agcgtcaatg ccgagcaagg acgaattcag gcccggctgt atgcagactg gctggaaaaa
     1441 gaatacggtc agcgtccggt catcttctac accaacggct atgatatctg gctgtgggat
     1501 gaccataaaa ctcatggtta tcccccgcgt cgggtgtttg gcttctacag caaggaaagc
     1561 ctgcaatatc ttattcagca gcgtgaaacc cgtctgccgc tgaacagagt gccacacgta
     1621 aaagataacg aaggtaaggc cgttgccgga cgtttgtacc agctcgaaac cattgcccgc
     1681 gtcagtgaac ggtttaccaa caagtaccga caatcgttaa ttgttcaggc tacgggtaca
     1741 ggcaaaaccc gcgtggcaat tgcactaagc aaactgatga ttgatgcccg ctgggtaaaa
     1801 cgcgtcctgt ttctttgcga ccgtaaagaa ctgcgtaaac aggcggcaaa tgcctttaat
     1861 caattcacca atgagccgct gtatgtggtc gggaaatcga aaaaagcaga caggcagaat
     1921 gccagaatct acatcgctac ctatcccggt atgatgaaaa tcatggacca tttcgatgtc
     1981 ggttatttcg atttgatcat cgccgatgaa tcccaccgct ctatctacaa cgtctacggc
     2041 gatctgttta agtattttga tgccctccag attggcctga cggccacgcc aattgacatg
     2101 gtgagtaaaa ccactttcgg cctgtttggt tgtgaaggac gtattcctac cgccaactac
     2161 agcctggaag atgccatcgc cgataacaat ctggttccct atgaggttgt cacacacaca
     2221 acagaattcc tgcgcgaagg tatcaaacgg gagaaattaa gcgacgcaca aatccgcgag
     2281 ctggaagaac aaggcataga tcccaatacg ctggagtttg atggcaaggc gctggatgag
     2341 gcgatctaca acaaagacac caaccgctat atcctgcgta acctgatgga aaatggcctg
     2401 aaagatcggg acggccagtt gcctggtaaa accatcattt tcgcccgtaa ccacaaacac
     2461 gcgctgctgt tgaatgaatt gttcgacgat atgtacccac agtttgccgg gcgcttctgc
     2521 caggtcatcg acaactacga tccccgcgcc gagcaactga ttgatgattt taaagggctg
     2581 gatgaaagca ccaacaaaga gctgaccatc gctatctccg ttgacatgct cgataccggt
     2641 attgatgtcc cggaaattgt gaatctggtc tttgccaaac cggtcaaatc aaaagtgaag
     2701 ttctggcaga tgattggccg tggtacgcgc ctctgtccgg ggttgtacgg ttacgacgat
     2761 aacggcaaac cgctggataa acagaaattc cgcattttcg atcactgggg caattttgag
     2821 tatcacgagc tgcataccga agaggcggaa gtcacagcga caaaatcgct tgcgcaaaaa
     2881 cgcttcgaag cgtgggttat gctgggggcc gctgcgcagc gtaaattcga caaacaggcg
     2941 gtggatttag ttgctcacca gcttcgcgag caaatcaacg cactggatga aaagtcgatt
     3001 gccgtacagg aaaaatggca gcagaaagcg cagtacagtg atgaaaaggt gctgcgccag
     3061 ctttctccga aaacacagca ggatctgcta tctgttctgg ccccgttgat gcagtggctg
     3121 gacgtgcgcg ggcaaagcga tgccatacgc tttgatatgg atattctggc ggcgcaaact
     3181 gcccgttata ccaacccgga agagctggat gttctctggc cgatcatcgt cgaaaaagtg
     3241 gagcgcctgc cgccgcattt ggcgcaggtg cagcaacagg gacaacgaat caatcagctt
     3301 cgtaatttaa gctggtggaa gcaggccagc ctggaagagc tggaggatat tcgcattcat
     3361 ttgcgcggta tcatgcacct gatggaaaaa gacgcgacgc ctaaatttgg ttcgatacag
     3421 gtggatatta ccgaagatgc gaacctgatc cagacggaga cccgcaaaac taatatccgc
     3481 tcgattgatt tcaaactcta tcgccagcag gttcaggggg cgctggagcc gctgttccag
     3541 caaaacccgg ttctgaagaa aatccgcaac ggcgagccag tcacgcaaaa cgagctggat
     3601 gagctggcga agctggtgct gatccagaac cccaacgttg atattcgtgc gttgaaagag
     3661 ttttatccgc aggcaaccgc cagcctggat aaacttttgc gtaccatcat cgggatggac
     3721 agcgacgcgg tggaagtgcg ttttgcccag ttcgccgctg ataacagcct gaccagccaa
     3781 caactgcgct tcctgtcatt gctgaaaaac cacattcgcg attacggcac cattgaaatg
     3841 cggcagctct ttgaacagcc ttttacgcat atccacaacg aaggcgttac cggtgtgttc
     3901 cctgatatag cgcaaatcgc ccgcctgcaa aagatagtcg aagagctggg tgttgtgacc
     3961 gacgcagcga cggtataata cacgcatgac aagccccggc agcggggctt ttgtatttaa
     4021 aacaggatat aaacgctaac aatgattact ggcgacttaa aaagtaaaat cgacggacta
     4081 tgggaagatt tctgggtggg tggtatcacc aacccgctga ccgtaatcga acagatcacc
     4141 tatctgatgt attcccggat gctggatacc caggaacaac gcgacgaaaa gcgtaaacaa
     4201 atcgcgggta ttgattttaa accgcgtttt gcgccagaac agcaggagtt ccgtttcagt
     4261 cactatagca accttggctc ggatgagatg atggaagtgg tgcgcgatgg cgtattccag
     4321 catttccgtc agctcggcca ggccgatgct tcgaaggtga cgctgctggg caactttatg
     4381 aaagatgctc gtctggagat tgttaagccg tcattgctga ccaaagcggt tgaggtaatc
     4441 aaaaacctgc cattggatcg cggcgacacc aaaggcgacc tttacgaata cctgttaagc
     4501 aagctgacaa ctgccggaat caacggacag ttccgcacac cgcgccacat tatccgcacg
     4561 atggttgaaa tgatggagcc gaacccggcc cgcggcgaga cgatttgcga tcccgcctgt
     4621 ggcaccggtg gttttctggc aaccagctat gaatatctgc tggagaagta cagctcgctg
     4681 gaatccattc atactgagat tggcaccaac gaacgtggcg agctggaaga gcaaaaaatc
     4741 tttaccggcg atctgctgac accgtggcgt aaccatgtgg ataacaacat gttccacggt
     4801 tacgactttg acaccacaat gctacgtatc gccgccatga acctgattat gcacggcgtg
     4861 gatgcgcctg atatccacta tcaggacaca atgagccaga gtttcagcac aaacttcccg
     4921 caggccagta aaaacgcctt caacctgatt ctggcgaacc cgccgtttac cggttcactg
     4981 gacgaggaag atatcgactc cacgctgtcg gcaatggtga aaaccaaaaa aaccgaacta
     5041 ctgttcctgg cgcgtattct gcaaatgctg aaagtgggcg ggcgcagtgc cactatcgtg
     5101 ccgcagggcg tgctgtttgg ctctagcaag gcgcaccagt cacttcgcaa aacgctggtg
     5161 gaagataacc aactggaaac ggtgatcaat ctgccttctg gtgtatttaa accttacgct
     5221 ggcgtggcga cggcgatcct gatctttacc aaaggcggtc aaacggatga ggtctggttc
     5281 tacgatctac aaaatgacgg ctacagcctg gatgataagc gcaacccgat aaaagacaac
     5341 gatctgccgc atctgctggc aagctggaag cattaccgta ctttacgcgg gctaccggtt
     5401 gataacttta tgggtaagaa gttagcctcg ttgcttaaac agcagtaccc ggaagggatt
     5461 aatgctggcg ttgattttaa agatcgcacg caggcggcgt ttgttgtacc gaaagcggat
     5521 attgctgcgc agaaatacga tctatccatc aaccgttata aagaagtcgt gtatcaggcg
     5581 gaggaatatg aagatccgaa ggtgatattg aagcggttaa aggatctgga aaaagagatt
     5641 ctggcggatt tggatgagct ggaggggatg ctgtgagttc ggcgatggtg aagttagggg
     5701 atgttattaa tgttcactac ggaaaagcat taaaagctga tcagcgtgta tcgaatggaa
     5761 gtgtccatgt gtttggtagt tcaggaattg tcggcaacca cgataaaacg ctttgcagct
     5821 atcccacgat aattattggc cgcaaaggca gcgttggtgc tattacctgg gctccaagcg
     5881 gaggctggat cattgatact gcttattacg ttgagatcaa agataataat aagttagatc
     5941 tccgatactt gttttatatt ctgagcggaa ttgatctcac taagaaaacg attaccacaa
     6001 gtattcctgg attaaataga gacgatctct atgatacatt tattaaatta ccaccttttg
     6061 aagagcagaa acgaattgtg gatcttctcg ataaagctga gggtatacgc caaaaacgtg
     6121 agcaatccat caaactggct gatgattttt tgcgtgctac gtttgctaca atgtatggga
     6181 atcctattac aaaccccaaa aaatggcctg tccatcttat gggagaaatt attgaattta
     6241 aaggtggaaa tcaaccacct aaaagtgatt ttatttttga acctaaacag ggttatatta
     6301 gattagtaca aattagagat tttaaatccg ataaatatgc gacgtacatt ccgcaagaaa
     6361 aagcaaaacg tattttcgaa gttgatgatg ttatgattgc tcgttatggg ccacctgtgt
     6421 ttcaaatact tagaggttta tccggcagct acaatgtagc tttaatgaaa gcttcaccca
     6481 aagaaaatat taggaagggg tttatattct atcttctaca acttccggaa tatcatgatg
     6541 ttgttgtaaa aaactctgaa agaacagcag gacaaaccgg tgtaaatctt gaactattaa
     6601 acaaattcaa tgttcccctc ccacctatat actatcaaga tgagatatta gatagattag
     6661 ctagaattga aaaatttaaa gaaaaaatag aaatatcatt aaatcattta gaaatacaat
     6721 ttttatcact acagaagaga ttaataggtt tttaaatcct tagtatcgaa ataaacatta
     6781 atttattata ttaaattaag atattttagg aaattaaggc gtcactctta ggtaacgcct
     6841 ttttttacta tctcgccccc taagaaggtt atatcttact taatacctgt gactattttt
     6901 gatataatca taaatacaca ctaatttgca aaaattactt gacaatacct gagtattcat
     6961 agcttcatct atatgttttt tcaatatttt gagaagctct cccatatcgc gcttgattaa
     7021 tccaggctcc atataaggca tcattcttaa aaagaaactg aaatcataag caagatataa
     7081 ctcattgacc cccaccttga cctcaggaac ggtttcagag cgtcgtttat cccatccttg
     7141 agactgaaaa ctaataggag tgatatttaa tcgtgataaa acgccacctg agagttctgt
     7201 ttcattaatt atactcccat caggattaat taatttagcg tgatttaaga agtgaaaaat
     7261 aggaatgcgc gcattgcctt tgcatatatt tggcaaaaca tcattaacaa cacatcccca
     7321 atcataatat ccattttcaa atacaacctc tcggatgaga tctacacgct ccagtccaac
     7381 caatccttta gttgccatcg attccgattt ttttaccatc gaaatagcac catatcccac
     7441 aacaaactca gttgtactga ccccttcttc aattttctct gaatccctca catataattt
     7501 tcctttcgga tcatcagtta agaccaactc ataaagttca tcttttatca ttcggaataa
     7561 atgtgctgga atcctttttt tagtttcact cagcacctca aatatttctt tataggatgg
     7621 tgcctgaaca tattttaccg
//
LOCUS       DAJTSL010000179         7598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTSL010000179 DAJTSL010000000
VERSION     DAJTSL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.179"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(319..1455)
                     /locus_tag="OO433_003566"
     CDS             complement(319..1455)
                     /locus_tag="OO433_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6051907.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGGLQSLTVVPGTDGVQGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1458..1820)
                     /locus_tag="OO433_003567"
     CDS             complement(1458..1820)
                     /locus_tag="OO433_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6051908.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2357..4270
                     /gene="mtlA"
                     /locus_tag="OO433_003568"
     CDS             2357..4270
                     /gene="mtlA"
                     /locus_tag="OO433_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6051909.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLT
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4500..5648
                     /gene="mtlD"
                     /locus_tag="OO433_003569"
     CDS             4500..5648
                     /gene="mtlD"
                     /locus_tag="OO433_003569"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6051910.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPSMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5648..6235
                     /gene="mtlR"
                     /locus_tag="OO433_003570"
     CDS             5648..6235
                     /gene="mtlR"
                     /locus_tag="OO433_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6051911.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6247..6456)
                     /locus_tag="OO433_003571"
     CDS             complement(6247..6456)
                     /locus_tag="OO433_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051912.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6741..7103
                     /locus_tag="OO433_003572"
     CDS             6741..7103
                     /locus_tag="OO433_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6051913.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1977 a         1878 c         1875 g         1868 t
ORIGIN      
        1 tagattaaat attttttaaa tgtgactgtc atccttcaga taataaagcc atcatcgcaa
       61 tagatggaaa agcactcctg ctcttttttg attagagccg tcgcaggagg aacaactcat
      121 atcatcattg cgatctcgac gatgtatagt ctcatcctgc ggcagaacaa gacggataaa
      181 aaatccaatg agagcacact catccagaac ttattaacat aatgaatatt aaagaaaaat
      241 catcgcagct aatgaagtaa tatgcaaaaa taattagtcc ttacaaggag caaagttcca
      301 taatctcccc cctccccctc aatgatccaa ataaagataa tgcatccagc tggtcattct
      361 tagcagcact ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa ccgccacctg
      421 ggcgtagatg ccgtcgggta aggcatcgat atcatcgtta gggtccagtt caatggttcc
      481 ctgcacaccg tccgtgccgg gcacgaccgt taatgattgc aatcccccct gcgcctgata
      541 agaaccgcct ggcacgacag gtaaaatact agtcagtttg ccgtgaaaca cctgcccagg
      601 tagcgcgtta aacaccactt ccgcatcatc gccgggtttc agacgtaaca gcgagttttg
      661 ccgaaattgg gcgacaattt gccgtttttg ctcggggatg aagaccatca ccggacgcag
      721 cggcaaggca gctgcgtatg tacctgggcg gatcagtacc tgagtaacgt agccattgct
      781 cggcgcgcga atgacagtct gctcaaggtt atattttgct tcagtaagtt gcgctcttaa
      841 gctcacaatc tgcgattgct cgccgttaac catactgtcg agctggctct ggatctgcgc
      901 ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg aggaaatttt gccgcgcatc
      961 gtcgatgtca cgttccgaga acggattcac cgccgcctgg ctgcctttca agtaacgttg
     1021 ataattttta aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg cctgcgcttc
     1081 agtgagctgc gcacgcagcg tctttatatt atgcgtcgcc gtcatcaggt cagcctgaag
     1141 tctgtcaact cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct cgcccttttg
     1201 aataagctga ttattcttgt cagtgacttc agtaacaatt cccgtcacct gtggcgtgat
     1261 agggatcgct atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt agttcatcaa
     1321 caaaatcaaa ccactcacca gaaacacgcc tcccagcgcc gccgtcgcca gcgtccactg
     1381 atttaccgga atgcggaaga ttttaaagac cgcccacgcc agcgccacgt aagttaaaac
     1441 aatcaataga tccataatta gatctccggg aacgtggaat agtcagtctt tttctcggcg
     1501 gcgagttgat gctccagccg ggaaattcgg tcagaaagtg aggcgatttc cgggtcagtc
     1561 gctttctcct gcgacgcaac atgtgactgc ataccccagc cacgctccgg ttgatagagc
     1621 gtcgcccaga tccacagaaa cggccagata acatgcagag taaacaggct cacccagccc
     1681 gccgtatgaa tagcgtcggc atgggggtga ttgcgctttt tggcaatcag ataagggatg
     1741 tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag cactcccaac
     1801 gcgaaatagt ttagaaacat atctgcctcg gattcacgtt tatcagtgtt gtttttgggc
     1861 tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg cgattctcgc
     1921 cataacacca aagaataatt tttagaggtg atgagttgct tagttacata acgattgtat
     1981 gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa atatcttgtg
     2041 attcagatca caaagattca acaaaccatc aaaacaaaaa tgtgacacta ctcacattta
     2101 aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt tacacaagcg ttttgtgatg
     2161 aacgtcacgt caattacctc tctacctcct atatttatgt gattaatatc acacaaaagg
     2221 ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac aaggtccggc
     2281 tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga cagcaaacat
     2341 aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct ttggtcgttt
     2401 cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc
     2461 gttatttatt ccaacagggt ggttaacgaa cgagacgctg gcgaagctgg tcgggccgat
     2521 gatcacttat ctcctgccgc tgctgatcgg ttataccggt ggtaagctgg taggcggcga
     2581 acgtggcggc gtagtcggtg ccatcaccac catgggcgtt atcgtcggcg cagacatgcc
     2641 gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta agcacttcga
     2701 ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata acttctccgc
     2761 aggcatcatc gggatgatcc tcgctattct ggcattcctc ggcattggcc cgattgttga
     2821 agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg acatgctgcc
     2881 gctggcgtct atctttgttg agccggcgaa aatcctgttc ctcaacaacg ccattaacca
     2941 cggtatcttc tcgccgctgg gtattcagca gtcccatgaa ctgggtaaat caatcttctt
     3001 cctgattgaa gctaacccag gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg
     3061 tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat
     3121 ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct
     3181 cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg tttctccggc
     3241 atctccgggt tctatccttg ctgtactggc gatgacacca aaaggggctt atttcgctaa
     3301 catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa
     3361 aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta tgcaggacat
     3421 gaaagctgag tctaaaggca catctccgct gtctgcgggc gatgtgacta acgacctgag
     3481 ccacgtacgt aaaatcatcg ttgcctgtga tgccggtatg ggttcaagtg cgatgggcgc
     3541 aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtcg cagatttcgg tcactaacag
     3601 cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc actcaccgtg acctgaccga
     3661 acgcgctatg cgccaggttc cgcaggcaca gcatatttcg ctgaccaact tcctcgacag
     3721 cggcctgtac accagcctaa ccgaacgtct ggttgctgcc cagcgccata ctgaaaacga
     3781 agtgaaagtg aaagacagtc tgaaagacag ctttgacgat tccagtgcta acctgttcaa
     3841 actgggcgcg gagaacatct tcctcggtcg caaagcagca accaaagaag aagcgattcg
     3901 ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat
     3961 gctggatcgt gaaaaactga ccccgactta tctgggtgag tctatcgcgg tgccacacgg
     4021 tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga
     4081 aggcgtgcgc ttcggtgaag aggaagatga cattgcccgt ctggtgattg gtattgcagc
     4141 ccgtaacaac gagcacattc aggttatcac cagcctgacc aatgcactgg atgatgagtc
     4201 cgtcatcgag cgtctggcac acaccaccag cgtggatgaa gtgctggaac tgttggcagg
     4261 tcgtaagtaa tccaatccca ccctctccac atggagaagg tggggttaat tgcctgatgc
     4321 gctacgctta tcaggcctac aggatgcatc acgatttgtt gaatttgcac gttcttgtag
     4381 gccggataag gcgcttacgc cgcatccggc gctgcccctc tcctcacgga gagggtttgg
     4441 gtgagggaaa agcctcaccc cagccctctc gggtaaaaac attgatgaag gttaatacta
     4501 tgaaagcatt acattttggc gcaggtaata tcggtcgtgg ctttatcggt aaactgctgg
     4561 cagacgcggg tatccaactg acgtttgccg atgtcaatca ggtggtactt gatgccctga
     4621 atgcccgtca tagctatcag gttcatgtgg ttggtgaaac cgagcaggta gataccgttt
     4681 ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt tgatctgatt gctcaggtcg
     4741 atttagtcac taccgccgtt ggcccggttg tgctggaacg tattgctccg gctatcgcca
     4801 aagggctggt gaaacgcaaa gaacaaggta atgaatcccc gctgaacatc atcgcctgtg
     4861 aaaacatggt acgcggtacc acgcagctga aaggccatgt gatgaacgcc ctgccggaag
     4921 acgccaaagc gtgggtagaa gaacacgttg gctttgtcga ttccgccgtt gaccgcatcg
     4981 taccgccttc ggcttcggca actaacgatc cgctggaagt gacggtagaa actttcagcg
     5041 aatggattgt cgataaaacg cagttcaaag gcgcactgcc gaacatccca agcatggagt
     5101 taaccgacaa cctgatggca tttgtcgaac gtaaactctt caccctgaac acgggtcatg
     5161 ctataaccgc gtacctcgga aaactggccg gtcatcagac cattcgtgac gctattctcg
     5221 acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga aagtggtgcg gtactgatca
     5281 agcgctacgg ctttgacgca gacaaacatg cggcgtacat ccagaaaatc ctcggtcgtt
     5341 ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg ccgtcagccg ctgcgtaaac
     5401 tgagtgctgg cgaccgtctg atcaagccac tgctcggtac gctggaatac agccttccgc
     5461 acaagaatct gattcagggg attgctggtg caatgcactt ccgcagtgaa gatgatccgc
     5521 aggctcagga actggcagca ctgatcgctg acaaaggtcc gcaggcggcg ctggcacaga
     5581 tttccggtct tgatgccaac agtgaggttg tatccgaggc ggtaaccgct tataaagcaa
     5641 tgcaataatg gtggaccagg cgcaggacac cctgcgcccg aataacagat tgtcagatat
     5701 gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt gtgcttgagc gtctgaatgc
     5761 tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag cttttgaccg aggcggtaaa
     5821 tcttctggtg cttcaggtat tccgcaaaga cgattacgcg gtgaagtatg ctgtagaacc
     5881 gttactcgac ggcgacggtc cgctgggcga tctttctgtg cgtttaaaac tcatttacgg
     5941 gttgggcgtc attaaccgcc aggaatacga agatgcggaa ctgctgatgg cattgcgtga
     6001 agagctaaat cacgacggca acgagtacgc ctttaccgac gacgaaatcc ttggaccctt
     6061 tggtgaactg cactgcgtgg cggcgttacc accgccgcca cagtttgaac cagcagactc
     6121 cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct gtgcgatcaa caatggtcct
     6181 ttcactgact gagctgattt ccaaaatcag cttaaaaaaa gcctttcaaa agtaagcaac
     6241 gtctgcttac tgcccctcta cctgcttcgg ccgataaagc cgacgataat actccagacg
     6301 ttgtagatat aacggcacgc tttcatcggg tattccggac ggaatcgcgt tacggggagg
     6361 aagttttttc agatactccc ggaacgcctg gcttgatgcc atgaaatcta cggctttatc
     6421 gataagaagc ggaacgtttt cacctaattt gcccatgatg atatctccgt attaccccgc
     6481 gccgggaatg cgcggccacc aattttagtt taggttcccg gtaaagatga cattaaggaa
     6541 aacgtgctga atcctcaata atcatccgct gctgtttatt atcttttttc tatagatttc
     6601 ttaattaatc aacgaattgc gtagtttttg agcatgttaa tgattacgtt gcagaactat
     6661 tacaatgtca tcagttgtga acaaagcacc tggtcgcgca tactaggggc tataaattta
     6721 tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg
     6781 atcgcctgga cgccatccgc caccagcagg ccgatctgtc gctggttgaa gccgcagaca
     6841 aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct cgcctgcgtg
     6901 aggttcatag ccaaaaactg agtaaagaag cacagaagct gatgaagatg ccgtttcagc
     6961 gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt gttcgcggac
     7021 tggtggttgt gcacccaatg accgcgctgg gccgtgaaat gggcctgcag gagatgactg
     7081 gattctctaa gactgcgttt taagcgcctc agacgactgg ttcgatcata tcaacccgca
     7141 tctggcttgc cttagctggt caatgcgggg actctcctgt ctactcgcct aattaccaaa
     7201 caattcactc cctttacgtc gatttaccgc tggttgatat tctgaaaatg catttttacc
     7261 gaaatagcca cctataaaca ataaaaatat atagaagata tcatttatta attaacaagc
     7321 agttacgtct ctttttttcg gatttaattg tgaaatttta atcatcatat tcatacaata
     7381 aattatctgt aaattttttc ccagacatgg tttctttcat atttgacatt tattaataat
     7441 catcattaga atccacgaaa ataatatatt catacaaaca cagacatctt ttgaaacgaa
     7501 tgatacattt ttatgatgaa tgcttaaaac aatatttaat cgacacacat cactgtcatt
     7561 aatgtaaagc aacataaatc accaataata caatggac
//
LOCUS       DAJTSL010000180         7517 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTSL010000180 DAJTSL010000000
VERSION     DAJTSL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.181"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..204)
                     /locus_tag="OO433_003573"
     CDS             complement(<1..204)
                     /locus_tag="OO433_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR6051914.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKED"
     gene            complement(198..1436)
                     /gene="preT"
                     /locus_tag="OO433_003574"
     CDS             complement(198..1436)
                     /gene="preT"
                     /locus_tag="OO433_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR6051915.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(1833..2072)
                     /locus_tag="OO433_003575"
     CDS             complement(1833..2072)
                     /locus_tag="OO433_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6051916.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(2075..2794)
                     /gene="sanA"
                     /locus_tag="OO433_003576"
     CDS             complement(2075..2794)
                     /gene="sanA"
                     /locus_tag="OO433_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6051917.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(2944..3828)
                     /gene="cdd"
                     /locus_tag="OO433_003577"
     CDS             complement(2944..3828)
                     /gene="cdd"
                     /locus_tag="OO433_003577"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6051918.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPYAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(3958..4653)
                     /locus_tag="OO433_003578"
     CDS             complement(3958..4653)
                     /locus_tag="OO433_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6051919.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYGSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(4650..5048)
                     /locus_tag="OO433_003579"
     CDS             complement(4650..5048)
                     /locus_tag="OO433_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6051920.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            5287..6237
                     /gene="dusC"
                     /locus_tag="OO433_003580"
     CDS             5287..6237
                     /gene="dusC"
                     /locus_tag="OO433_003580"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6051921.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            complement(6624..6707)
                     /gene="yohP"
                     /locus_tag="OO433_003581"
     CDS             complement(6624..6707)
                     /gene="yohP"
                     /locus_tag="OO433_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6051922.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            6931..>7517
                     /locus_tag="OO433_003582"
     CDS             6931..>7517
                     /locus_tag="OO433_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HCR6051923.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEW"
BASE COUNT         1944 a         1929 c         1884 g         1760 t
ORIGIN      
        1 atcttctttc accagatgat caaaacgcgg cgagacttcg ttggcgataa aaaagccgat
       61 cgttttaaac accacaccgc cccaacctgt gtcgtaggct ttggcacaca tctcatagca
      121 gttgcctacc ggcgaagaag agaggcagaa cgggttggga aacttcacgc cgcaaaaagt
      181 aatcgaaaga tctttcgtta acatgagcaa gctccctcta aatagtgatg aatcgcctcg
      241 gcggcttctt tcccggtttt cacggcatag accatggttt tgtcaccctc aacaatatcg
      301 ccagcagcaa agacttgcgg gtcgcgggtc tggtaatttt gtgttttgat ggtgttacgc
      361 tgcggctcta actccgcaaa ggcatctagt ctggcatgct gaccgacggc gagaataatt
      421 ttatctgccg ccatcgtcag ttcgcccgat aaccgtacat gcttaaacgt gactttattg
      481 ccttccacgg ctactggcgt gaatccatcg atgatcgaaa cgcccagctc ccgggcgctg
      541 gtaaactctt tttcgcttgc cggaaactca tctaactctt cacgcgctac gcaagttacc
      601 gcctgacagc cgagaacttt cagcgtgctg gctacgtcca tcgtgacatc accgccgccg
      661 ataattaatg cgctttgtgg aatgctgata tcgccttgcg cctgtcgtgc acgttgcaag
      721 aagtcgacgg caatctcaac gtcactatgc tcaaacagcg gtagcccgga accgcttgat
      781 aaccccacgg tgaccagtac cgcgcggttt tctgctttca gctgctcaag ggtgagtgtg
      841 ttaccgactt cgttgttgca cttaatggtc acgcccattt tttcaatacg ggcgatctct
      901 gcatccagta ctgattgcgg taaccggaat tgcggaatgc cgttacgcaa ccagccaccg
      961 ggttgtgctt ctttctcata gatcgtgacg tcataaccct ggtttgtcag tgtcacactg
     1021 gcctgcaatc cggcaggacc tgcgccaata atcgcgactt tgccgagcgt tttagtaccg
     1081 ggctgataaa tttccatccc ggtttgttgt tcaaaatcag taacaaaacg ctgtaagcgg
     1141 ccgatatcaa tgggagcatc aacaccggca cgggtacaac cgctttggca taatttctcc
     1201 gtcgggcaca ctctggcaca aacggcaccg agggcattat tttcgcgaat tgtctcggca
     1261 gcgcctttaa aattacgaaa gtagattgag cgaataaatt tccccggatc ggtctgtgcc
     1321 gggcaagcct gactacaggg agcgtcgtga cataataaac agcgagaggc ttctttaatc
     1381 gccagtaacg gcgtaaaagc cggagtgagt tcatccagat aattttgttg cggcatgtga
     1441 tcttccttaa tgctatgagt aattaaggcg aacgctaaat atactcgtca tacttcaagt
     1501 tgcatgtgct gcgtctgcgg gttattcggc ccatccatgg gcctcacccc ttatggggcc
     1561 gctgcaggca gcgttcaaat ccgctccatg cggatttgtc gctcacccca gtcacttact
     1621 tatgtaagct cctggggact cactcacttg tcgccttcct gcaactcgaa ttatttagag
     1681 tataaatatt gcgtgctgat attaaggcta tgcgcgtggg gaaatttata ctgtgaaagg
     1741 attcacaaat agctgcggtt cagaatgtta tatccgcagc ttattgagtg aagtaataat
     1801 tattctcaat aattaacaga aaagagaaaa tattaacgat agaaaataag ataaacaatc
     1861 atgccgatag ataaaattcc gaagccgata tatgccacca tataagcgaa aaagagtccg
     1921 ggattttttg ctcgccagac atacaccggt tccggtgcat ttttaacccg tttatcaatc
     1981 gcgcctgcgc gccagccttt ccatgcttcg cggaaacaaa atatcggaat aaggaaaaaa
     2041 acggcaacga caaaaaactg ctgtacatcc ataactactt tccttgtttc ttttgtaatt
     2101 caagtaactg ttcgggtgtg acggcgggat acccctgcgc atcttccggc acctggtgca
     2161 tagccggaat agggaccagc ggccctaaaa aacgcggttc acgtttaaaa atataaaggt
     2221 cagccagcgc accgaaacgg gcggcaaatt cgcgaatacg tactgacagc atatctttcg
     2281 gtgacggtac ggcataacac tgagcctgaa tccccatatg cagcgcaata aataatgccc
     2341 gctcacagtg gaaacgttgg gtgataataa tgaaatcatt agtatcgaaa actttgcgtg
     2401 tacgcacgat ggaatccagc gtacgaaagc ctgcgtaatc gagaacaata tctgatgggt
     2461 cgacaccagc agcgattaaa tctttgcgca tggtcatcgg ctcattataa ctttgcaatg
     2521 cgttatcgcc gctcagtaat agataattta ccttaccgct gttataggca ttaatcgctc
     2581 cttgaatgcg gtagcgataa tactgattaa ttacgccagt acgataatat tttgctgttc
     2641 cgagcaccac accgacctgg cggtagggga gatcctgcaa ttcgtcgtag atataaggcg
     2701 cggttttcca gctcatccag cgatcgaggc cgagcacagt caacaacagc aagccgatca
     2761 ggactaacag gctgaggaac acgcgcttta acatgcagga gactcgatct aaggaaaatt
     2821 tccatcaggc tactttaccc gctggttttg cgcaagaaac ggcaaactgt ttgccgggaa
     2881 tttcgacacc aggagagggc tggcaagccg gacaaggcgt tcacgccgca tccggcacca
     2941 ggcttaagcg agaagcactc ggtcgatact gtgacagccg agagctttca gcgttgcgga
     3001 ggtggcatcc cactgaatca acggcgcatc ggctttttct gccagaaccg cgcgctggat
     3061 atccgggtaa tcataaccct tgagattcaa cagaattaac gctccctgca acggtggcag
     3121 agtcgggttg aatgcggcgt tttcagcgta gctgccactg aaaatacgac cgtctttaca
     3181 ttccagcgcg acaccgcttg gcgacttact gtaaggcatg tgcgaacggt ttgccgctgc
     3241 aatcgccgcc tgagaaagcg catcaccagt cagcgcatag ccgtgatcct gctcgtccat
     3301 cagcagcgtt ttaatctcca gatctttcgg cccaaaggca tacggcagat agtcacgcag
     3361 cgcgtgtgcc tcgcggcccg gcagatgaat acgcagatcc agaccgctgt tcagttcatt
     3421 cataaactga cggcagtgac cacaaggcgt gtagttaacg gtgatggctg caagcgcttt
     3481 ttcaccactc aaccaggcgt ggctgatcgc gctttgttcg gcatgaacgg tttgctgcat
     3541 tgtcgcacca ataaattcca tattggcacc gaaataccag gttccgctca caccgcgcgc
     3601 aattgcgcca acattaaaat tcgacaatgg cgtacgcgca caggccgctg ccagcggaag
     3661 tagtgcgaat gccagcgcgt cttcgtccag ccccgttgcg ctcttcagcg atgagacttg
     3721 ctccccggtc aacaaagcgg ggaagtactt gtctgccaga ataggttcca gtgcagattg
     3781 caagttatcc gcaagttggg caaaagcggt ttgaaaacgt ggatgcatgg cgttgcctca
     3841 taatcatgta atgggataac agtttacgaa cccgttatgg ctttatatgt gatctgaatc
     3901 tcattaatta tgcaacgcgt gaaacttaca tcaaaaatgc gcgacgcatc gcaaatttta
     3961 gcccattact gccagaataa tcgggaaaag gaacggtgcg atcagcgaag taattatccc
     4021 gcataacacc agcgctagcg aactaaatgc accttcctga taatccagct cggcgcagcg
     4081 cgccgtaccg agggcgtgcg aggcagtccc cattgccaga ccgcgcgcag ctttggtacg
     4141 aatacgcatc gcattaagca atgtatggcc aaataccgcg ccgaggatgc cgacgaaaat
     4201 cacgcaaact gcgctgattg ccggaatacc gccaatactg ccgccaaccg ccattgcaat
     4261 aggcgtggtg actgatttcg gcaggattga cgcggcgatt tccggtgaag cgcccatcaa
     4321 caatgccacg gaagtccccg tcaccatcgc aaccacgctg ccgataaaac agatggtgat
     4381 gatcgatttc cagcgcgcgc ggatctggtg tagctgctca tataaaggat aggccagcgc
     4441 gacgaccgcc ggttgcagca gatcgtttag cacttcgcta cctttaaagt agctgccgta
     4501 agagatgccg gtcagcatta aaaaagggat gatcaccacc attgctacca gcaacgggtt
     4561 aagcaaggga aacttatacc gtgccgccag tttgcgggcg gcaaaaaaga cgatcaatgt
     4621 cagtggtaat gaccaccaga tattagccat cattcttctg atcctttctg acctacgact
     4681 ttacgttcac cgtgcaccag ttgcgaactc cagctcacca ccagaaaaac caccagtgta
     4741 ctgacagcac aggaaaccac taccgggcca aactgtgcgc ggagcaaatc aaaatattgc
     4801 atgacgccta cgccaatcgg cacaaacaat agcgccatat agcgaatcag tacgtagcac
     4861 cccggattga cccattttgc cggaagaatt tgcaaggcca gcaggacaaa caggatcagc
     4921 atcccgatga tgctgcccgg aatggttacc ggtagcaggg aagcaatgaa aatgcctgca
     4981 tacaggcagg cataaatcag gacgaaagcg cgtaaatatt gccagataat gttcagtgtc
     5041 ttactcataa tcaacacccc agatgagatg aattcatcat acaattaatt cgtaaaacgt
     5101 gctaccgatc acattaagaa atcagcgctt attttttcgc tccccggcat tacgcaatgc
     5161 gcaacgaact caggcataca tcaagcatag agagggatta cagtttgcag caatccctct
     5221 ttgcagacct ttaatccgtt gaccataggc gctaacatag cgcctcattt ttttgcgggt
     5281 gaagatatgc gtgtgttact ggctccgatg gagggtgtgc ttgactctct ggtgcgtgaa
     5341 ttgctgaccg aagttaacga ctacgatctg tgcatcaccg agtttgtccg cgtggtggat
     5401 caactgctgc cggtaaaagt ttttcatcgc atttgccctg agctacaaaa cgccagccgg
     5461 acaccatccg gtacgctggt gcgcgtgcag ttgttgggtc agttcccaca atggctggca
     5521 gagaatgccg cccgtgcggt ggaattaggt tcctggggcg tggatctcaa ttgcggctgc
     5581 ccgtcgaaaa cggtcaacgg tagcggtggt ggggcgacgt tactcaaaga tcctgaactc
     5641 atctaccagg gtgcaaaagc gatgcgtgaa gctgtaccgg cgcatttgcc cgtcagcgtg
     5701 aaagtgcgtc tgggctggga cagcggtgag aagaaatttg aaatcgccga tgcggttcaa
     5761 caggctggtg ctacggagct ggtggtgcat ggacggacaa aagagcaggg ttaccgcgcg
     5821 gagcatattg actggcaggc gattggcgag attcgccagc ggctgaatat tccggtgatt
     5881 gccaacggtg aaatctggga ctggcagagt gcgcaacaat gtatggcgat cagcggctgc
     5941 gacgcagtga tgattggtcg cggggcgctc aatattccca acctgagccg ggtggtaaaa
     6001 tataacgaac cgcgaatgcc gtggccggag gtggttgctt tgctgcaaaa atatacccgt
     6061 ctggaaaagc agggcgatac cgggttatat cacgttgcgc ggattaaaca gtggttgagt
     6121 tatttgcgta aagaatacga tgaagcaacg gaattatttc agcatgttcg ggtgttgaat
     6181 aattcccctg atattgcaag ggctattcag gcaattgata tcgataagct gcgataatag
     6241 tggaatcgca gtttatcaga gataatgaat cgcttattca gcttcatcat cttgttgcgc
     6301 tcctttcatg agctaagcca catattgcca ctggcgcaag gagcgcgcag ggggaggaca
     6361 atcgcctcct ccccctgccc cctgggctct ggcgaacaaa atcgccgctg cgcggtacct
     6421 tcagcttatc cccttcggct accgggtcgg gcgcgaggta acgtccttgt aagacgcgcc
     6481 ctcagcccac atccatgtgg gctgccccgg ccttcgggga acgcttcagc gattttgacg
     6541 ccaccaacac cggagctgtt tgcaaatcaa gtacacaaaa tatctagttc ctgacctgac
     6601 aacttatatg ccatatctga tttttaaaat atcatcttaa atacgccagt caccaccagt
     6661 agcccaatca ggaaaataat caatacagcc cagagtataa ttttcattgg cgttccttta
     6721 tttgtgagtg ttgaataagt ataggaaaaa acgaagccct ttgccgttat ccataaacga
     6781 ttaagataaa tcggaagata tcattcactt agtctatatt tttctgggta tgtcgtttta
     6841 ttgctactga tgtattgcca cgcgaaacta aaaaatatat cgcaacacac ggtaatctcc
     6901 gcgcaccgtt tcttacctga ttattccccc atgaatcgtg attcctttta tccagccatc
     6961 gcctgttttc cgctgttatt gatgctggcc gggtgtgcgc ctatgcatga aacccgccag
     7021 gcgttaagcc agcaaacgcc cgctgcacaa gttgacaccg cattacccac ggcgctgaaa
     7081 aatggttggc cagacagcca atggtggctg gagtatcacg ataatcagct cacttcatta
     7141 attaacaatg cactacagaa cgcaccggat atgcaggtgg ctgaacagcg cattcagcta
     7201 gctgaagccc aggcgaaggc agttgccact caggatggtc cgcagatcga cttttcggcg
     7261 gatatggagc ggcaaaaaat gtcggcagaa ggcttaatgg ggccgtttgc tctgaacgat
     7321 ccggccgcag gtacgaccgg cccgtggtac accaacggta cttttggctt aacggcgggc
     7381 tggcatctcg atatctgggg aaagaatcgg gcggaggtta ctgcccgcct gggtacggtt
     7441 aaagcacggg cggcggaacg cgagcaaacc cgccaattgc tggctggcag cgtagcccgc
     7501 ctgtactggg agtggca
//
LOCUS       DAJTSL010000181         7475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTSL010000181 DAJTSL010000000
VERSION     DAJTSL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.182"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..341
                     /locus_tag="OO433_003583"
     CDS             <1..341
                     /locus_tag="OO433_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6051924.1"
                     /translation="QTEAFLNAYGLSRFAPLGYDPRDLPIRDLAGYRKKGNHDGDPII
                     FYTFPAAFEQEIAKGFNTKQFAEVLKNAGMLTPPTSGRGYQGRVREDGRQIRVYVLNF
                     MAEESSQPEE"
     gene            716..1150
                     /locus_tag="OO433_003584"
     CDS             716..1150
                     /locus_tag="OO433_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6051925.1"
                     /translation="MTAQISAYGRLVADPQTRTTTNGNNMAMARLAVSLPCNAAEAGE
                     STFWLGVIAFGKQADALAKHQKGDLVSVAGNMQLNQWTGQDGGTQQGYQVVADSVISA
                     RTVRPGGKAGQRGQATDALRRAQQPSRDEYDQRPPFDDETPF"
     gene            1161..1802
                     /locus_tag="OO433_003585"
     CDS             1161..1802
                     /locus_tag="OO433_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051926.1"
                     /translation="MSTQPIIIEFGSYRWPRHLAIHEADHAIAAWFFGIQEVEIALSG
                     NNRVAKTYLSGDVDECGAVAVWHANYPRNVAEVKSYTLSNDDTRLRLIDQVCRAMVIS
                     LAGIVAQSRYTGDDINALMETSGKADMKNVRDLSEVYRVAGGEDADIQRISLSRAEAL
                     IALKWWDVIALAKILENRNHMPASNFQRIMGDIDKPVPKPMTLAELDELVGEE"
     gene            1799..2149
                     /locus_tag="OO433_003586"
     CDS             1799..2149
                     /locus_tag="OO433_003586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051927.1"
                     /translation="MKPYILAAGLISSAPALSAIHCGGFTIQPDRYRTTINGDVVHIV
                     DAQFYGAPRDFSQAAIKLLPDAITTTDRQYRIETQGGNALLELMTRENPPRVLNRERC
                     DAGLTSFGWYTSAP"
     gene            2843..3454
                     /locus_tag="OO433_003587"
     CDS             2843..3454
                     /locus_tag="OO433_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051928.1"
                     /translation="MRDRTRSYTTDLPRIGLPFLANMRSRLADAEPGTQLYTQTESGT
                     LYAFRQADSYAMTINGVTRAIRTTTTKAGYGVREWYACPHCMKRAAKLYIGKKDIGCR
                     ACWKLHYKSQSADILDRMRMKIRQQRCAIWGSNDLANNLFNDIRMFPKPKGMRWATFD
                     RKRAELSVMEMAYWQAFSPVVDRITGVIERKAGKAARGIGLTL"
     gene            3992..4534
                     /locus_tag="OO433_003588"
     CDS             3992..4534
                     /locus_tag="OO433_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCR6051929.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSELHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNDGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPVENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSSINDRLQTLN"
     gene            complement(4527..4886)
                     /locus_tag="OO433_003589"
     CDS             complement(4527..4886)
                     /locus_tag="OO433_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051930.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGETNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            5320..6144
                     /gene="dinD"
                     /locus_tag="OO433_003590"
     CDS             5320..6144
                     /gene="dinD"
                     /locus_tag="OO433_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6051931.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQNILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            6436..7053
                     /locus_tag="OO433_003591"
     CDS             6436..7053
                     /locus_tag="OO433_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6051932.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7050..7475)
                     /gene="ligB"
                     /locus_tag="OO433_003592"
                     /gene_synonym="yicF"
                     /pseudo
     CDS             complement(7050..7475)
                     /gene="ligB"
                     /locus_tag="OO433_003592"
                     /gene_synonym="yicF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+;
                     internal stop; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
BASE COUNT         2000 a         1835 c         1909 g         1731 t
ORIGIN      
        1 agcagacgga ggcgtttttg aatgcctatg ggctgagtcg attcgcgccg ctgggttatg
       61 acccgcgcga tttacccatc cgtgatttag ccgggtacag gaagaagggg aaccacgacg
      121 gcgacccgat aattttctac accttcccgg cagcgtttga gcaggagatt gcaaagggct
      181 tcaataccaa acagtttgcc gaagttctaa agaatgcagg aatgcttaca ccaccaacga
      241 gcgggcgagg ctatcagggg cgagtaaggg aggatgggcg acagattcgc gtctatgtac
      301 tcaatttcat ggcagaggaa agcagccagc cagaagaata attatttctt cacatgcgta
      361 gttttagtgt tgtctcggtt gtctttgttg tctcaatttg taattaagat tgatttataa
      421 ggattttatt ttaatttctg agacaacacc gagacaacat ttagccattt tgagacaaca
      481 gggcgaaagt gtgtttttag tctggcaggc agtgagacaa catttatgcc gggtgagaca
      541 acacgaaatt gcaaatgttg tctcagcaaa acccagacgt ggcgcggcct gtagagccgt
      601 gagacaacaa agacaacaga gacaacacta tttttgctta ctatgaaaaa aaatcggcag
      661 gtgtcccaca ttaagaaatc gtcattagca aaactaataa tccagtgagg ggaaaatgac
      721 agcacaaatt tcagcatacg ggcggctggt ggcagatccc cagacccgca ccacaacgaa
      781 cggtaacaac atggctatgg cgcggctggc ggtgtctctc ccctgtaatg cggcagaggc
      841 gggagagtca accttctggc tgggcgttat tgcttttggg aagcaggccg atgcgctggc
      901 aaagcaccag aaaggcgacc ttgtgagcgt ggcaggcaat atgcaactca accagtggac
      961 agggcaggac ggcggtacac agcaggggta tcaggtagta gccgacagtg tgatcagcgc
     1021 cagaacggta cgccccggcg ggaaggcagg acagcgggga caggcaacgg acgcgctgag
     1081 acgtgcgcag cagccatccc gtgatgaata cgaccaacgc ccaccattcg acgatgaaac
     1141 gccattctga gagggaagat atgagtacgc aacccatcat tattgagttc ggcagctatc
     1201 gctggcccag acatcttgca atacatgaag cagatcatgc gatagccgca tggtttttcg
     1261 gcatacagga ggtagaaatt gcgttatcgg gtaataaccg agtggcaaaa acctacctta
     1321 gcggcgatgt tgatgaatgt ggagctgtcg cggtatggca cgccaactat ccacggaatg
     1381 tggctgaggt taagtcttac accctctcga atgatgacac caggttacgt ctgatagacc
     1441 aggtatgccg ggctatggtt atctctctgg ctggtatcgt cgcgcaatca cgctatacgg
     1501 gggatgatat taacgcactg atggaaacgt ccgggaaagc agacatgaaa aatgttcgtg
     1561 atttatcaga ggtataccgg gtggctggtg gggaggatgc tgatatacag agaatatccc
     1621 tttcccgtgc tgaggcgctg attgccctga aatggtggga tgttatcgcg ctggcgaaaa
     1681 tactggagaa ccgtaaccat atgcctgcca gcaacttcca gcgcattatg ggtgatattg
     1741 acaagcctgt accaaagcct atgacgctgg ctgaactgga tgaattggtg ggtgaagaat
     1801 gaagccatac attctggcag ccggcctgat ctcctcggca ccggcactat ctgctatcca
     1861 ttgtgggggc tttaccatac agccagaccg ttatcggaca acgattaacg gcgatgtggt
     1921 tcatattgtt gatgcgcaat tttacggcgc accgcgagac tttagccagg ccgccataaa
     1981 gttattgccg gatgccatta ccactaccga taggcaatac cgcatagaga cgcagggagg
     2041 caatgccttg cttgagttga tgaccagaga aaacccgcca cgtgtgctta atcgtgagcg
     2101 ctgcgatgcc gggttaacaa gtttcggttg gtacacttct gcgccgtgat gttcgttccg
     2161 agcgcagcct aaaatattgg actgttcttg acggatttaa aactctaatg gagccaagtc
     2221 cttgccagta gcgggctagg agcctaattg tgattcaggt cgtcaaaaat attcaggttt
     2281 gcggcattgg ttcgcagtac gcagtccggt acgcggcaaa actgcgcatt taacgtgcgt
     2341 accacctgac gaattctaac gccggcattg caaacagatg cgtgcgcaaa atggctaaga
     2401 cgtctgatat tgctggcttt ggctcaatca gtttggaggt ggcggtatca gtacgatgct
     2461 aatttttgct aaggttttag tacgcaactc aggtacgcac tctcaccaga tacgaaaccg
     2521 attcgcagtt gcgtaccagc ctgtaaggcg cattatcgct gggtttctac agtcgaatgc
     2581 agtacgcaaa gtggaaaatc ctccgaatac gaaacccgag cattgcgcag taaattgcga
     2641 attgcgcagt taacaaaata tctccgcaaa acgaaacgga tatcaatgcg caaaccatcc
     2701 tcaacccccg cgcggttact ggctttattg atgtgaaatt gcaaaattgg tgcgcaattt
     2761 acagtaaaat acgcatatta tttcgtattc aatttgcgca ccgttcgcag ttaatgcgca
     2821 gtaattcgca ggaggccagc cagtgagaga cagaacgcgc agctatacca ccgacctgcc
     2881 ccgtataggg ttgccattcc ttgcgaacat gcgtagcagg ctggcggatg ctgaaccggg
     2941 tacgcagctt tatacccaga ccgaaagcgg aacgttgtat gccttccggc aagccgacag
     3001 ctacgccatg acaatcaacg gcgtgacgcg ggctattcgc accacaacca cgaaggccgg
     3061 gtatggcgtc cgggaatggt acgcttgccc ccactgtatg aagcgggctg caaagctgta
     3121 tatcggcaag aaagacattg gctgccgggc gtgctggaaa cttcactata aaagccagag
     3181 tgccgacata ctggacagga tgcgcatgaa gatacgccag cagcgatgcg cgatatgggg
     3241 cagcaatgac cttgccaata atctgtttaa tgacattcgc atgttcccaa aaccaaaggg
     3301 gatgcgctgg gctacgtttg acaggaagcg ggcagagtta tcagttatgg agatggctta
     3361 ctggcaggca ttcagcccgg tagtggacag aatcacgggc gttattgagc gtaaagcagg
     3421 aaaagcagcc agaggcattg gcctgactct ataaaagcaa aacgcccgca cggggcgtca
     3481 gagaacggga tagacttctg cgacaatttg cattcgccgc caatatagca agccgtccac
     3541 atcaagcaat aaactatcac cgggaggggg aggccaaatc tctgcaatcc tgagccttaa
     3601 ggactgcccg ccccatcgtt ttttcatacc cgcgaaaaat gaaatttaat ccgggcgtgt
     3661 ttcatccttc cagagggtat gacgtatgac accacgcgaa atagccttat tgaccatcgc
     3721 caaacttgag catggaggcc accagcttac acaggcagat caacgggaga tagaacgctc
     3781 agttaatgcc gatatagccc ggcgcgacag gttccgcgaa atgatgcgag cacctgccta
     3841 ccagtggaag aagccagcgc cgcgcaggta gatacgggat tattccgcat gtaacttatg
     3901 cgtattaagc aaatttcctt tattcacctt tacagaattg tgagtacgtc agtatcgtta
     3961 tccttccgat tcgcatttat tcaggatttg catgtaccag ctaaagataa ccatccgaga
     4021 cagcaaacca cctatatggc ggcgtgtgct ggttccagag caaatcccct ttagtgaact
     4081 tcacgcggtg atccagttgg cttttggctg gaatgacgaa cacctgtata tgttcgagaa
     4141 agggcgtaaa ggtgatcccg gtagcgagta tcgcgtatgg ggtgaggatg aaagcatggg
     4201 taatgcggca atcacaccac tatgggcagc gctccagaat gatggtgaca agctggttta
     4261 cacgtatgac tttggcgact ggtgggattg cgtcattgtg ctggagaagc aaacacacga
     4321 tacgagcaat cagcccatta gctgcctgcg tgggaaaggc accaccccgg tggaaaactc
     4381 aggcggcttg catggttaca atgaattgct attacaggcc agagagtctg ataatcctga
     4441 gcaggccgag atccacaatt tcctgatgct ggatattgag cgccgggttt acgacctgag
     4501 cagcatcaac gacagattgc agactcttaa ctgacccgca tatattcgcc tgatattgcg
     4561 ttatcagcgc cgccagtatc gcgcataaca gcgcactgac cgggcaaacc gtagcgcacg
     4621 accgttgcgt tagtctcacc gccgcgccgt atctggcgcg attcatacac tgtcacccgc
     4681 tcaagcagtc caccagcaac catgctttcc agtgtgcgcc gggttgattc gagctggtga
     4741 cgcttatcga acgacaccat gccatgaagc aggtaggcca cacccgacac atcgaacggc
     4801 ggcgcaccaa tctcaccagt cacccattcg aggttatccg gttcaaagta gctaagtatc
     4861 tcttttttgc ggctggtcat tctcatagct ggcaggttcc ttattgtggg atagcactat
     4921 catacaataa ggcgcaaaag ataagaggcc agaacagcaa gcatgagagc ataaaacgag
     4981 cagccaaaga cagcgcctca aaataccgtg tacacaaggg tgtacctact gagaaaacgc
     5041 attacaagaa acacagcaac cacgggcgtt taaagccact actcaataaa cagattcaac
     5101 tcgtaacgcc aattcttact tttcagcctt cacaaatgcc gcccactcaa acagagcggc
     5161 atttttcttc cccgcaactt caattctgtt ttgcgtgcct gctccagatt ttgcgatgtt
     5221 tttttgccca gcacactgag aacgtgagat actcacaact gtatataaat acagttacag
     5281 atttactttc tttgcaatcg atatcacatg gagtgggcaa tgaacgaaca tcatcaacct
     5341 tttgaagaaa tacggcatta tggcactgaa gggcaagaat tttggtcagc tagagaattg
     5401 gcaccgttac tggattatcg ggactggcgc aattttcaaa aggtactggc ccgcgcgact
     5461 caagcgtgcg aagccagcaa tcaagctgca tctgaccatt tcgttgaaac caccaaaatg
     5521 gttgtattag gttcaggggc tcaacgagaa cttgaagatg ttcatctctc ccgctatgcc
     5581 tgttacctgg tagtacaaaa cggcgaccct gcgaaaccgg ttattgcggc agggcaaact
     5641 tattttgcta tccagacccg acggcaggag ctggctgacg atgaagcttt caggcaactt
     5701 cgtgaagacg aaaagcgtct ttttctgaga aatgaattga aagaacataa caaacaattg
     5761 gttgaggcag cgcagcaagc tggggtagcg acagctatcg atttcgccat atttcagaat
     5821 catggttacc gggggctgta tggtggatta gatcagaaag ctattcatca gcggaagggg
     5881 ctgaaaaaga atcagaatat cctggatcat atgggttcaa cagaactggc ggctaatctc
     5941 tttcgagcta cccaaacaga agaaaaactc aagcgggatg gcgtgaattc aaaacagcaa
     6001 gcaaacacca cacactttga cgtgggtcgc aaggtgaggc aaaccattca ggaacttggc
     6061 ggaaccatgc ctgaggagtt gccgaccccg caggtcagca tcaagcagtt ggaaaacagt
     6121 gtaaaaatta cagagaagaa atagcagaaa ctaaaaactc tttttcttaa ttgagatacc
     6181 cgatgcgtaa gccaaagtcc aggtgcagtg aacacaatgg ctaaacattg cacctttctt
     6241 ttccacccct caattttaac ctattttttc ttatgcattt tctcagacaa gaactcagaa
     6301 taatgcatct acgctacaga aaatagcgat ttcacataac tacaagttat caatttcctc
     6361 ttaccttaaa aaatctcaat cgtgacaatg cgcacaaatc gctaccctgc cagacagatt
     6421 tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcatcac tgccgaagcc
     6481 atgacggggg cgctggcggc cggacgacgg cgcatggata catttggcgt aattattatt
     6541 gcgaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg ccactatccg
     6601 ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc agtactcacc
     6661 actatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct cgatgcgctc
     6721 ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg gcacggccca
     6781 attattgccg ttgtcgcggc ggtaaccacc ggcgtgtttg gcggcgtttt gcgcgatatg
     6841 ttctgtaaac gcatcccact ggtattccag aaagagttgt atgctggtgt ctcatttgcc
     6901 tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt ggtgattatc
     6961 tccaccctgg tattcggctt ttttgcccgc ttactggcgc tacgtcttaa gctgggatta
     7021 ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat ctgctgggca
     7081 gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat aacctgtctg
     7141 gcgcgcccag atccagtccc cggcagctgc tgtcagaact gctccgtgct aaataaaagt
     7201 tgcgactagg accgttcatc actggcatta agcgccgccc gggttagcgg tattcccatt
     7261 gccatcaccc agcgagtaaa aggctgctta cgagccagat taaactgatg ccataactgc
     7321 gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt taataaaagc
     7381 caagaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc ggcctcacca
     7441 atgccatcca gcccaagaac ctgttttgac cccag
//
LOCUS       DAJTSL010000182         7376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTSL010000182 DAJTSL010000000
VERSION     DAJTSL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.183"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..270)
                     /locus_tag="OO433_003593"
     CDS             complement(<1..270)
                     /locus_tag="OO433_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6051933.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEILKKATAL"
     gene            943..1227
                     /locus_tag="OO433_003594"
     CDS             943..1227
                     /locus_tag="OO433_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6051934.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1215..1700
                     /locus_tag="OO433_003595"
     CDS             1215..1700
                     /locus_tag="OO433_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6051935.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            1734..2912
                     /gene="ltrA"
                     /locus_tag="OO433_003596"
     CDS             1734..2912
                     /gene="ltrA"
                     /locus_tag="OO433_003596"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6051936.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVKHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNTIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2995..3149
                     /locus_tag="OO433_003597"
                     /pseudo
     CDS             2995..3149
                     /locus_tag="OO433_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(3203..3685)
                     /locus_tag="OO433_003598"
                     /pseudo
     CDS             complement(3203..3685)
                     /locus_tag="OO433_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3941..4921)
                     /locus_tag="OO433_003599"
     CDS             complement(3941..4921)
                     /locus_tag="OO433_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6051937.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(4921..6120)
                     /locus_tag="OO433_003600"
     CDS             complement(4921..6120)
                     /locus_tag="OO433_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6051938.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(6442..6780)
                     /locus_tag="OO433_003601"
                     /pseudo
     CDS             complement(6442..6780)
                     /locus_tag="OO433_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(6847..>7376)
                     /gene="istB"
                     /locus_tag="OO433_003602"
     CDS             complement(6847..>7376)
                     /gene="istB"
                     /locus_tag="OO433_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR6051939.1"
                     /translation="APQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIAMGYEAVR
                     AGIQVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQKEAKLF
                     FQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIKGESYRL
                     RQKRKAGVIAEANPE"
BASE COUNT         1946 a         1675 c         1758 g         1997 t
ORIGIN      
        1 gagcgcggta gcctttttta atatttcatt ctccatttca atgcgttgta gctttttcct
       61 cagcttacgt atttcgattt gttctggtgt tatcggagag gcttttggtg ttttgccctg
      121 acgctcatca cgcagttgtt tgacccatct tgtcattgtg gaaaggccaa catccatagc
      181 tttggcggca tctgccaccg tgtatttctg gtcaacaacc agttgagcgg attcgcgttt
      241 aaactctgcg ctaaaatttc tttttttcat tggagcacct gtgttgttct gaggtgagca
      301 tatcacctct gttcaggtgg ccaaattcag tgtgccactt cagggcggca tccggcatat
      361 aggcagcatg aacgcaacac aggctctcgt acggaacgtg ggaacctgtc gtctcgatgc
      421 taagggagcg gcgcggagca gaggcactgc aagcgcctga gtaccgatgc gagacacagg
      481 ggcagatggc ttcgtagtag tgaggaagtg cctgtaatgg gcatggagcg aagggggtca
      541 gtcgaggcgt ttgaggttac caatcgaagc tatacccggt aatggcgcag gttgacctgc
      601 acttggccaa gtggattgcc cgcaaacaca agcgagcgcg aggtagtctg gtgcgtgcat
      661 tcgagtggtt actgcgaatt cgtcacgact gcccgacgct gtttgcacac tggtgcttag
      721 cttacgatac ttgaatggtt caacgacaag agccgtatga cgcgagagtg tcacgtacgg
      781 ttccgtggga gcgtaggggg gcggtgcccc tgcgccaccc gatatcccct ttgagtgcgg
      841 ttacgtccac agaggtaaat gccgctgacg cggcatctgt aatctgttac tttgtgtaca
      901 tcatttgcta tatattgtca atatgctatt gttgtaaagc aaatgatata cggaggcttt
      961 atgaaatcag gtgttcagct caatctcaga gcaagagaat cacagcggat actcattgat
     1021 gctgctgcag aaattcttca taagtcgcgt acagatttca ttctggaaat ggcctgtaaa
     1081 gctgctgaag atgttattct cgaccggcgg gtgtttaatt ttaatgacag gcaatatgaa
     1141 gagttcattg aaatgcttga tgctccggtt gcagatgatc ctgccatcga aaaattgctg
     1201 gcaaggaaac ctcaatggga tgtgtaactg caccagaacc tttatcctca tttcatcagg
     1261 tcgctgaatt tgtcagcggt gaagctgtac ttgatgactg gttaaagcag aaggggctaa
     1321 aaaatcaggc aatcggagcc acccggactt ttgttgtatg cagaaaaggt acgcaacaaa
     1381 tagtcggttt ttactcactg gccactggta gtgtcaatca tacagaagct acaggtaacc
     1441 ttcggcgcaa tatgccagac cccattcctg taattatact tgcacgcctg gctgtagatg
     1501 tgtcatttcg aggaaaaggg cttggcgcgg atttacttca tgatgcagtt cgtcgttgtt
     1561 acagggttgc cgaaaatatc ggtgtgcgtg caattatggt tcatgcgctt acggaaaacg
     1621 caaaacagtt ttatattcat catggtttta agccatcaaa aactcaggtg caaacattgt
     1681 ttcttaaact ccctcaatag ggagtaatgc actggtctgg gcatcatatc tggatgtgcg
     1741 tcgcaacaag ggagcccccc ggatgcgacg ggcagacttt gaaaatgttc gaccagcaac
     1801 gtgatggcaa tctatacaag atctggaacc ggctatgttc gggaacatgg tttccgccac
     1861 cagtgctgga aaaacggatc ccgaagtcga atggcaagga acgcattctg gggatcccaa
     1921 cagtatccga tcgaattgct cagggagcga taaaactttt catggaagaa aagcttgacc
     1981 cgattttcca tgctgattca tatggttacc gaccaggcaa atcagctcat gacgctctga
     2041 aacaatgtgc catccggtgc tggcgttata gctggatact ggaagtggac atcagtgcgt
     2101 ttttcgacca tgtgaaacat gatctggtgc tcaaagctct ggaacaccac gggatgccta
     2161 aatgggtcat cctgtactgt cgacgctgga tggaagcgcc aatgcagagt tgtgaaaatg
     2221 gagaattaat aacccgaaca cgaggcactc cgcagggcgg ggtcattagt ccgctacttg
     2281 ctaacttgtt ccttcactat gcatttgatt tgtggatgga aagagaatat cggggggtac
     2341 cgtttgagag gtacgctgac gatattgtag tacattgttc acgaatgagt gatgcaacaa
     2401 ggctaaagaa caggttatcg gagcgcttct cggaagtcgg gctggtcctg aatgcaggga
     2461 aaacgaatat cgcctacatt gacacgttta aaaggagaaa cgtcgcaacg agtttcacct
     2521 ttctcggata tgacttcaaa gtgcgtacgc tgaagaattt caaaggcgaa cggtaccgaa
     2581 aatgcatgcc gggtgcgtca aatgcagcaa tgcgcaaaat aacagaaaca atcaagaagt
     2641 ggcgtataca tcgctcaaca gctgagagtt tgctggattt tgcgaggcgc tacaatacga
     2701 tagtgagagg ctggatcgag tactacggaa agttctggtc cagaaatttc aactatcgac
     2761 tgtggagtgc aatgcagtca cgtctgctca agtggatgca gtctaaatac agactttcga
     2821 accggaaggc tcagcgaaag ctgacgctgg taaggaagga gtatccgaag ctatttgttc
     2881 actggtattt actacgtgca tcaaatgagt gatcaagagc cgtatgacgc gagagtgtca
     2941 cgtacggttc cgtgagcagc cggggagtga aattctctcc ggctgactcg actcgcgggt
     3001 tccgacagcg atgacgaaca tgcggcggtg ttgtactcgc tgatcggcac atgccgtctg
     3061 aacaatgtgg aaccagaaaa tggctgcgtt acgtcattga gcatatccag gactggccgg
     3121 caaaccgggt acgtgatctg ttgccctaga aagttgatct gagctctcag taaatatcaa
     3181 tacggttctg acgagccgct tacttttggc gtaattctgc tgcgccttta ctgaacacaa
     3241 tgccgtattc agcaagaagt ccacgaatgt gatttatcag ttgtgtcctt tccattgaaa
     3301 ccaaccgacg catattcaaa aaagtttgga cgtcctgttg ctccagagtt tttattggta
     3361 cgggtcgtat agttccatgt tggcaggcct cagctattgc ctgtgcgtcg ttataatcat
     3421 ttttctggca ccgctgataa gctttaacat gttgtgccgg aagcaatatt acatcatggt
     3481 cgagtttcct gatttcccgg gcccagtaat gagatgcccc gccagcctcc atcgcaacag
     3541 tcattttagg ctgttgcacc agccagttaa gcagttgatt gcgagaaagt ttggtctttc
     3601 ctgcgggttt gccatgagca ttgatactga aaagataaaa gttcgtttta gccagatcaa
     3661 tgccggtgat tttctgttca gacatggtat cccgcttact tttgtggtta aagtttatcc
     3721 atcatggcgc aattgaaggc tgccgtgaaa gagggggaag actatctcat cggttactgt
     3781 taaacggcta agagaaacta tctttttcaa gcaaagaaga tacggtgaaa caggcgattt
     3841 caccgtgaaa tactgttcaa aaaaaacgct aataaaagca aaatttcttt taaaacagcc
     3901 aattaaaaac tggtgagttg ctggcgaaaa acttgaattt tcacagcgaa ttcagattat
     3961 cttttagtac aagagcaatc atacgatcta acttatcctg aacttccaac ggtaatctgc
     4021 cgaattcata tgagaacgta cgtcccttta ctcgttttcg agcaaattta tctttactat
     4081 caaatttcca taacaaagtt acaaccgctt tatcctttac tcctgaatca agcaatgaag
     4141 acatttcctt ggttataagc cgcataatac tgtttttaac ctcgtcttca gaaagattgt
     4201 catcagcagt aataagctca atactcggcg agatattctt cacaacttca tcaatagata
     4261 tatcacccag cctcagtcgt tcacttaatt cagccagttg cctgtaatcg gcgtatgtca
     4321 gctcagactg aacaggaaaa agagacacaa aatctttagg tacggacgct gcttggaggg
     4381 cacgagtcac cttagctgcc gacaatccct ctctttctgc aatttgcttc tggttcatac
     4441 cggcctcttt gagacgtact aaacgcaacc caatttcacg aatattatgt tcaagtgacg
     4501 tctgcaaatc tttcgccaga tgctgagctt cagaaacagt aagctcatca tccgttacaa
     4561 gtacacgtaa tccaaccttg catagcaaag ctgctgcccg acgacgggaa ccatccaaaa
     4621 tctcaataag atcgccagtc cgaatcccaa tacatggata gaattgttgt aaacgtatag
     4681 ttctggtgat gtcttttagt gagtccggcg tcagggctgt ctgttcgcgg ccattcacct
     4741 cttcaaccac aaaagtttga gtgtccactt tgtcatgatc aaccttgatc tcagtaaatt
     4801 ttgcctttcg gccagtattg agggtaaata catgaactgg agccgatatt tcttcagtat
     4861 tattcaggat atttgtattc agcgtacggc caatagtcgg tcggtgcttt ctgttttcca
     4921 tcagtagttg gcccttataa attcaatacg atcaaaaact gctttagcga aatcttctgc
     4981 tgccatacgt gcatttttca aagcttcacc actgccaaca taagtaacag gattcgcaga
     5041 tatcacggta tcaaacgact cgccgcttct ctcaaaacca tcaagacgtg gcattgaaac
     5101 atcaagcatg tcaccaccga aaatctcttt tgtcaggcta tggcagtatt tatgatctga
     5161 cttattcgcc agtttcgaca tgaagccaat atttgcctgc aaacggcatg aacatccatc
     5221 ctgttcaata atctgtacaa gttcaggtaa acgagccaaa tatttaagcg tggaatggaa
     5281 atcaacttgt gctggcggca ccggtgtaaa catgatatct gcggcagcaa tggcattttt
     5341 caaaaatgcg tccagatgcg ggcctgtatc aataaggatg aagtcgaaat catgcttcag
     5401 cttatcaata atattctcac gcaatatcgc gtgtttgttt tgtcctaata gatgctcctc
     5461 gcacagagta tcccagtttg atgcaataaa ggcgtcgtca atagaagccg gcataacata
     5521 aacgccaggg ataactgacg gaacgataaa atcttccagt aattcctcac gactcacgtt
     5581 ctgcaacata gcctgcggtg cggttgtatc aaccagcccc accgcatgca agtagttcag
     5641 aaacatagtg gctgatgact ggggatctaa atcaaggagc agaatacgta aatcttctga
     5701 cagcaggtga ggatgagcac gcagggcgtg agctacggaa acactactca cggttttaga
     5761 cacaccgcct tttagactac cgatgaatat ggaatatgcc tcactataac gatcgcgata
     5821 tttaggaatg ccacgatgtg catagatatc gataatgttc tggatggtta atgcatattt
     5881 tgtcgcggtt cctgctgggc gtttttcaaa ctgatagccc tgagcctcca tctcaccgac
     5941 tgcaccatcc acgcttgcgc gcgttaattt tggaagctta gctacagcag actttgaata
     6001 gacttgataa aaaacatcag cctgaaactc ctgcttttgc tcctgaatct gttttgttag
     6061 ggcgagcaac attttatctg cacgctgcgc aaccttactt aactgctcaa aactagtcat
     6121 tccccctccg tgctatgtag gtttttaact tattacaaaa aaaactgcat tagcagggta
     6181 atgcatgcaa gtgcatattg tacatgctta tattaaaaat gcgcgcattg ctgatttatt
     6241 aatcatcgga atgaatatta ctgtaagata gtatgccttt ccgggagaaa gacgctgatt
     6301 tcccccgaat aatccagttc accaccacac tccggacaac agctttcttc aggctccaga
     6361 tggtgtattt cgcgggaaac atgttccggt aacggacggc gatgacgtga ctgtcgcaac
     6421 aggcgggggt agtgcttcca atcagtagaa catgtgcttt tcaatgaagg agccgatgac
     6481 tttttcgtgg atttccacgg aacgcgagaa gcagattgtt ttacgtgcca gccgcttgat
     6541 gcgggtgcgg agggtccggt tgttacgttc aatacgttgc gtgaagattt tgccggtcag
     6601 gtgtttctcc ttcggcactt ctctggcgta gctgccccag tcatcgctgg tcaccatgcc
     6661 gatacagaac ggagtcaaca gggctaacag ttcccgacag gtctcatcat tccgtggccc
     6721 gaaagtgtaa gccaacacac cgccagtttt agtgttgtac gcataccaga gccagtgttg
     6781 tgtcaacgac ggatgaaaag tgatccactt atatctccac caacggccca atattgatcc
     6841 accgttttac tcaggattag cttctgctat aaccccggcc tttcgtttct gtctgagtcg
     6901 atagctttct cctttgattt gaacgacatg tgagtggtgt aagatacggc ccagcatcgc
     6961 tgaggtcagt gctgcatcac cggcgaacgt ttgatcccac tgcccgaacg gtagattgga
     7021 tgtcaggatc attgcgctct tttcataacg tttagcgatg acctggaaga acagttttgc
     7081 ttctttctga ctgaacggca gatagcctat ttcatcaatg atgagcaggc ggggggccat
     7141 tactccacgc tgaagcgtcg ttttataacg gccctgacgt tgtgccgtag ataactgaag
     7201 taacagatct gctgctgttg tgaagcgaac ttggatacct gcacggactg cttcatagcc
     7261 catcgcgatt gccagatggg ttttccccac acctgatggc cccagtaata cgatattttc
     7321 attacgttct atgaagctga gtgagcgtaa cgactggagt tgcttctgcg gtgctc
//
LOCUS       DAJTSL010000183         7292 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTSL010000183 DAJTSL010000000
VERSION     DAJTSL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.184"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..125
                     /locus_tag="OO433_003603"
     CDS             <1..125
                     /locus_tag="OO433_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6051940.1"
                     /translation="IGRESFSVDRITPDDGGCSYIRLRREGAGNVRAGGYYEGA"
     gene            112..699
                     /locus_tag="OO433_003604"
     CDS             112..699
                     /locus_tag="OO433_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6051941.1"
                     /translation="MRGLENAIRNLNSLDTRMVPQASAWAVNRVAAKVVSVATRQVAR
                     NTVAGDNQVKGGPLKLVRQRVRVFKASPAGKMYARIRVNRGNLPAIKLGTTCRRAGDG
                     LKVGKYFFRGAFIQQLANGRWHVLRRLPEACFETGKDSKGRPRKNRLPVEVVKIPLAG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRIHLG"
     gene            696..1097
                     /locus_tag="OO433_003605"
     CDS             696..1097
                     /locus_tag="OO433_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6051942.1"
                     /translation="MNRHTQIRQAVLASLKGECGGNVVLFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            1108..1848
                     /locus_tag="OO433_003606"
     CDS             1108..1848
                     /locus_tag="OO433_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6051943.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTLATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1907..2293
                     /locus_tag="OO433_003607"
                     /pseudo
     CDS             1907..2293
                     /locus_tag="OO433_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
     gene            2302..2630
                     /locus_tag="OO433_003608"
                     /pseudo
     CDS             2302..2630
                     /locus_tag="OO433_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            2602..5643
                     /locus_tag="OO433_003609"
     CDS             2602..5643
                     /locus_tag="OO433_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6051944.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAAGDAERSSARMQ
                     RFMERQTQAARQTMHAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEEFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAVGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIGINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5643..5972
                     /locus_tag="OO433_003610"
     CDS             5643..5972
                     /locus_tag="OO433_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6051945.1"
                     /translation="METFRWKVRPDMNVVSEPKVVAVKLGDGYEQRRPAGLNNLLSTY
                     SVTIRVRKGEHPPLKAFLERHGGVRAFQWTPPYDWKPIRVACRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5972..6671
                     /locus_tag="OO433_003611"
                     /pseudo
     CDS             5972..6671
                     /locus_tag="OO433_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            6676..>7292
                     /locus_tag="OO433_003612"
     CDS             6676..>7292
                     /locus_tag="OO433_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6051946.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQL"
BASE COUNT         1716 a         1683 c         2384 g         1509 t
ORIGIN      
        1 tgattggccg ggagtctttc agcgtggatc gtattactcc tgatgacggc ggatgtagtt
       61 atatccggtt gcggcgtgaa ggagcaggaa acgtaagggc agggggatat tatgaggggg
      121 cttgagaatg ccatccgcaa tctgaacagt cttgataccc gtatggtgcc gcaggccagc
      181 gcatgggcag taaaccgggt tgctgcaaaa gttgtctcgg ttgccacccg gcaggtcgcc
      241 aggaataccg ttgcgggaga taaccaggtg aaggggggcc ccctgaaact ggtgcgtcag
      301 cgcgtccggg tatttaaagc cagtccggca ggaaaaatgt atgcccgtat ccgtgttaac
      361 cggggcaatc tgcccgccat taagcttggc acaacatgcc ggcgtgccgg tgacggactg
      421 aaggtgggga aatacttttt tcgtggtgcg tttatccagc agctggcgaa cggtcgctgg
      481 catgttctca ggcgtcttcc tgaagcgtgt tttgaaacag gaaaggacag taaggggcgg
      541 cccagaaaaa atcgtctgcc cgtggaggtt gtgaaaattc cgctggccgg accgctgaca
      601 caggcatttg aggatgcccg cgaccgcatc attgccgcgg aaatgccgaa acagctgggg
      661 tatgccctga aacagcaact gaggatacat ttgggatgaa caggcacaca caaatccgcc
      721 aggcagtact ggcaagcctg aagggcgaat gcggggggaa tgtcgtgctt tttgacgggc
      781 ttccggcatt tattgatgcg caggaactgc ccgccgtggc ggtgtggctg agtgatgcgc
      841 agtacaccgg aaaaatgacg gatgaggacg actggcaggc tgttctgcat attgccgttt
      901 tcgtccgtgc acaggcgccg gattctgagc tggatacgtg gatggagaac accatatttc
      961 cggccatgaa ggatattccg gtgctttccg gactcatcga caccatgatc ccactcggtt
     1021 ttaactatca acgtgataat gagatggcca cctgggcgat ggcggaaatc acgtaccaga
     1081 tcacgtacac gaattaagga ggtggcaatg accacaccaa atccactggc aaagacgaaa
     1141 ggtgcgggaa cgacgttctg gatgtacacc ggcaagggcg atgcgtttgc gaaccctttg
     1201 tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg cgaaatgacc
     1261 gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa aacgacaacc
     1321 caggggcaga aatccgtcgg tgatactttg gcgacgctgg cctggcgtcc gggtgacagc
     1381 gggcagaaaa aactggttca gttgttcgat tccggtgaag tctgcgcgtt tcgtatcaaa
     1441 taccccaacg gtacggttga tgttttccgt ggctggctga gttcactggg taaaaccatt
     1501 acctcaaaag aggtgatgac ccgtactgta aaaatcagcg gtgtggggcg tccgtatctg
     1561 gcagaggagg gcgctgaaac cgtgggtgtt accggactga cggtggcacc ggcatctgtc
     1621 agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga cggggccagt
     1681 gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac cctgagcggg
     1741 cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg catgacctct
     1801 gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca ggacgatact
     1861 catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt ttctgaaaca
     1921 ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg ggctgcagag
     1981 aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac aggagggaga
     2041 actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata tcaatgcctg
     2101 gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga cgctttgagc
     2161 atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga gggttctgtc
     2221 gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg cgctgacgcc
     2281 ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga gttccggcgg
     2341 gcagactggc ggcggatgct gtcggaaatg tcggccactg agcttggtga gggggcgatt
     2401 atttccggat gcagagcttc agtgatgtgt ggatggatgc gcagtttgcc tcgctgaagg
     2461 cattgatcgt gagaatggtg tccggcagca gtgatgctgc ggtggctgat ttcagccttt
     2521 taccggaaga gaacgggata ccggagcgaa cggacgaaga actgatgcat cttggggaag
     2581 gtatttccgg aggtgtgcgt tatggaccag atagccaacc tggtcattga tttggggatt
     2641 gatgcggcag agtttaaaaa tgaaattccc cgtatcaaaa accttctgaa tggtgcagcc
     2701 ggtgatgcag aacggtcttc tgcccgtatg cagcgtttta tggagcgtca gactcaggcg
     2761 gcccggcaga caatgcatgc ggcttcttcg gctgcaacag cagcatcagc ccatgcgcag
     2821 acggtggaga agaatgcacg ggctcatgaa cgcatggccc gtgaggtgga acaaacccgt
     2881 ctgcgcgtgg atgccctgaa tcagaaaatg cgcgaggaac aggcgcaggc cagggcactg
     2941 gcggaggcgc aggataaagc ggctgccgcc ttttatcgcc agattgacag tgtgaaacag
     3001 gccggtgcgg ggcttcagga attacagcgt attcagcagc agatccgaca ggccagaaac
     3061 agtggcgggg ttggtcagca ggattatctg gcgctgattt cggagatcac ggcgaaaacc
     3121 cgtgccctga cgcaggcaga ggaacaggcc acccggcaga aagcggcgtt tatccgccag
     3181 cttaaagagc aggcaacccg ccagaatctg tcgtcttctg agttgcttcg cgccagggcg
     3241 gctcagctgg gggtaagcag tgctgcagaa gtgtatatcc gcaaaatgga gcgggcagga
     3301 aaagccacac attcgctggg gctgaaaagt gcggcagccc ggcgggagct gggggtgtta
     3361 atcagtcaga tggcgcgcgg caattttggt gcgctgaggg gatccgggat aacgctggct
     3421 aaccgtgccg gatggacagg cgcactgatg tcgccgaaag gcatgatgac tggcggcgtt
     3481 attggcggac ttgtcgcggc ggtcctgggt ctgggtaaag cctggcatga cggccggaag
     3541 gagggcgagg aatttaaccg tcagctggcg ctgacgggac attatgccgg tgtcactgtc
     3601 gggcagttgt ggaaactcag ccgggccata tccgggaatg gtatcacgca acatgcggca
     3661 gccggtgcgc tggcacaggt ggtgggcagt ggggcatttc atggaaacga tattggtatg
     3721 gtggcgaaag ctgccgcaca gatggagcga tcggttggcc agtcggtcag cgataccata
     3781 aatcagttta agcggctgaa ggatgatcct gtaaatgccg cgaaggcgct ggacaatgcg
     3841 ctgcattttt tgactgccac tcagcttgag cagatacgcg tccttgggga gcaggggcgg
     3901 tccagtgatg cggcccggat cgccatgtct gcgctggcag aggaaaccgg taaacgcaca
     3961 tctgatattg ataataatct caatgcgctg ggtagtacgc tgcaaacctt gtctgactgg
     4021 tggaagcagt tctgggatgc ggccatgaat attggtcgtg aagactcgct ggatgcgcag
     4081 attgatgcgt tacaggaaaa aattcagcgc gcgaaaaaat atccgtggac aaacgcctcc
     4141 acacaggtgg agtacgatca gcagcgtctt aacgatcttc aggagaaaaa acgccggaag
     4201 gatttgcagg atgcaaaagc gcaggcagaa cggaattacc aggagcaaca gaaacgccgg
     4261 aatgctgaaa atgccgcgct gaaccggatg aatgaaacgg aagctgcacg acatcagcgg
     4321 gaaattgcgc gtattaatgc catgcagtac gccgaccagg ctgtcaggga tgcggcgata
     4381 cagcgtgaaa atgaacgtta cgaaaaagcc attaagaaaa atacacgggc aacccgtaat
     4441 gatgaggcca cccggttatt gctgcagtac agtcagcagc aggcacaggt ggaagggcag
     4501 attgctgctg ccagacagtc agcaggcatt gccactgaaa ggatgactga agcgcataaa
     4561 cagcttctgg ccctgcagca gcgcatcagc gacctggacg ggaaaaagct gacggcagat
     4621 gaaaagagtg tgctggcccg taagaatgag ctgattcagg cgctgacgct gctggatgtg
     4681 aaacagcagg agctgcagaa acagacagcg cttaacgacc tgagaaaaaa aacggttcag
     4741 ctgaccagcc agctggcaga caaagaacgt gcactgcgtg agcagcacaa tctggatatt
     4801 gccactgcag gtatggggga taagcagcgg cagcgctacc aggcacagtt gcgcatccgg
     4861 caggaatacc ggcaacagtt gcaacagctt gagaatgaca gtcgccagaa aggcacttac
     4921 gggacggagg actaccggag ggctgaggag gtgctgaagg ggagcctgaa gcgacaactg
     4981 aatgaaaaca aacgctactg gcaggaactg gaagtggcgc agggcgactg gaaaaacggt
     5041 gccatgcggg cgtttcagaa ttttacggcg gatgcggata atgcggtggg aacggcagaa
     5101 cagatgttta cagtggcatt cagcagtgcc ggtaatgcac tggcgacatt ctgtaccacc
     5161 ggtaagctga attttaaatc cttcacctct tccctgttgt cagatatggc cagaattatg
     5221 gcacagatgg ccatgatgca ggcggtaaag ggcgtcggct ctttattcgg cttcacgact
     5281 aatgctgatg gcggtgttta ccagtctgct gatttgagtc gctacagtgg cacggtggtt
     5341 aaccgtccga cgttttttgc ttttgcaaaa ggcgcgggtg tgatggggga agcgggacct
     5401 gaagccattc tgccactgcg tcgcggtgct gatggtaagc tgggggttgt ggcggatact
     5461 ggtggttcag gcatggtgat gtttgccccg cagtacaaca tcgggatcaa taacgacggc
     5521 acgaacgggc agataggtcc ggcagcactg aaggtggttt atgacctcgg gaaaaaagcg
     5581 gccgcggact ttatgcaaca gcaggcccgt gatggtggcc ggttaagtgg agcataccgg
     5641 taatggagac gtttcgctgg aaggtgcgcc cggatatgaa tgtggtatca gagccaaaag
     5701 tggtggcagt gaagctgggt gatggttatg aacagcgtcg cccggcggga ctgaataatc
     5761 tgctgtcgac ttacagcgtg acgatacgtg ttcgtaaagg tgaacaccca cctttaaaag
     5821 cctttctgga acggcacggt ggcgtccgcg catttcagtg gacgccacct tatgactgga
     5881 aaccgatcag ggtggcttgt cgtaaatggt cggcaagcgt gggggcgtta tgggtgactg
     5941 taacggccga ttttgaacag gtggtgaact gatgcaggat atccggcagg aaacactgaa
     6001 tgaatgcacc cgtgcggagc agtcggccag cgtggtgctc tgggaaatcg acctgacaga
     6061 ggtcggtgga gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac
     6121 ctggcagggg cgacagtatc agccgtatcc cattcagggg gagtggtttt gaactgaatg
     6181 gcaaaggcac cagtacgcgc cccacgctga cggtttctaa cctgtacggt atggtcgccg
     6241 ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt aaggtttacg
     6301 cccgttttct ggatgcggtg aacttcgtca acggaaacag tgacgccgat ccggagcagg
     6361 aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc agtgcctctt
     6421 ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt atcatgctgg
     6481 cgaatacctg tatgtggacc taccgttctg atgagtgtgg ttacacgggc agggcagtgg
     6541 ctgacgagtt cgacaaacca acgacggata tccggaagga caaatgcagc aagtgtatgc
     6601 gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc atcaataaac
     6661 tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc ccggcgatgt
     6721 gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggggagcg gtatatccct
     6781 tgtgtgaata tttctgcaga gccggaggcg tattttcgta tcgcaccgga agactggctg
     6841 cgggcagaga tgcaggggga gattgtggca ctggtccaca gtcatcccgg tggtctgccc
     6901 tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg gtggctggtc
     6961 tgccgggggg cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg gcgctttgag
     7021 cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc ggggattgag
     7081 atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct ctatctggat
     7141 aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc gggcgatgtg
     7201 ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg cggcgacggt
     7261 gagctgctgc accatattcc tgaacaactg ag
//
LOCUS       DAJTSL010000184         7283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTSL010000184 DAJTSL010000000
VERSION     DAJTSL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.185"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            168..275
                     /locus_tag="OO433_003613"
     CDS             168..275
                     /locus_tag="OO433_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051947.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(328..1086)
                     /locus_tag="OO433_003614"
     CDS             complement(328..1086)
                     /locus_tag="OO433_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6051948.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVTYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1373..2302
                     /gene="pfkB"
                     /locus_tag="OO433_003615"
     CDS             1373..2302
                     /gene="pfkB"
                     /locus_tag="OO433_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6051949.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2403..2693
                     /gene="ghoS"
                     /locus_tag="OO433_003616"
     CDS             2403..2693
                     /gene="ghoS"
                     /locus_tag="OO433_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6051950.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            2799..3659
                     /locus_tag="OO433_003617"
     CDS             2799..3659
                     /locus_tag="OO433_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6051951.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(3700..4236)
                     /locus_tag="OO433_003618"
     CDS             complement(3700..4236)
                     /locus_tag="OO433_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6051952.1"
                     /translation="MTYQQAGHIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4383..5051
                     /gene="hxpB"
                     /locus_tag="OO433_003619"
     CDS             4383..5051
                     /gene="hxpB"
                     /locus_tag="OO433_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6051953.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5214..5792
                     /locus_tag="OO433_003620"
     CDS             5214..5792
                     /locus_tag="OO433_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6051954.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQIINTLQIQFHRLIKH"
     gene            5937..>7283
                     /gene="tcyP"
                     /locus_tag="OO433_003621"
     CDS             5937..>7283
                     /gene="tcyP"
                     /locus_tag="OO433_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR6051955.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDK"
BASE COUNT         1832 a         1774 c         1799 g         1878 t
ORIGIN      
        1 tattttcgat atatatgttg atgcataata ttattttttc ataaaacgca gaaacagtga
       61 ccaggtgtac aacgccaccc aacggctgta ataacaaaaa atcccttgat gcccgtccct
      121 tttgttacac tccgttatca cgcacaagag atatgcagga cactggaatg ccgactaaac
      181 gctttgataa aaaacactgg aagatggtgg tagtgctact ggcaatctgt ggcgctatgt
      241 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
      301 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
      361 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtaa cgttgtaagt
      421 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
      481 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
      541 ccatgcataa gccccggagg catagcccag cggctgagtt tcactggcat tgtcggtata
      601 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
      661 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
      721 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
      781 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
      841 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
      901 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
      961 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1021 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     1081 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     1141 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     1201 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     1261 tatatatatt tatctgcaaa attttaaata aagctccaat aaatcatatt gttaatttct
     1321 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     1381 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     1441 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     1501 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     1561 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     1621 agaagccaaa gactggaccc ggcagaattt acatgtacat gtggaagcaa gcggtgagca
     1681 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     1741 gcaagttctg gaaattgaat ccggggccat cctagtcata agcggaagcc tgccgccagg
     1801 tgtgaagctg gaaaaattaa cccaactgat ttccgcttcg caaaaacaag ggatccgctg
     1861 catcgtcgac agttctggcg aagcgttaag tgcagcactg gcaattggta acatcgagtt
     1921 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccgga
     1981 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     2041 ttcccttggt ccacaaggag cgctgggtgt tgatagtgaa aactgcattc aggtggtgcc
     2101 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     2161 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     2221 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     2281 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     2341 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     2401 ctatggccag tggcgatctt gtccgttatg tcataaccgt catgttgcat gaggatacat
     2461 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     2521 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     2581 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     2641 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     2701 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     2761 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     2821 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     2881 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     2941 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3001 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     3061 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     3121 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     3181 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     3241 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     3301 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     3361 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     3421 ttttcgaccc tgcctgctac tggggtgacc gagagtgcga cctggcgatg ttaccgctgc
     3481 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     3541 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaaggttat
     3601 ttggcggtca gcatttggtt attgctcagc agtcactgga tagattatta gcagcatgat
     3661 atgggttgaa gataatggcc gctctgtgcg gccttttgat taaataaacc caagcagaga
     3721 aaagaacaca taacctgcag caatacaaat aaccggcagg atatacagtg agaaaaactg
     3781 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     3841 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     3901 cgcctggcga ctcattcggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     3961 gaaaatcacc cagaacataa cgttcacgcc accattaaaa ttgggcgtcg gggagttata
     4021 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagtaaa
     4081 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     4141 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     4201 caaaacagca atgtgtccag cttgttgata cgtcatgagt tctccttgat taagacgcat
     4261 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     4321 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     4381 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     4441 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtggata
     4501 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     4561 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     4621 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     4681 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     4741 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     4801 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     4861 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     4921 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     4981 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     5041 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     5101 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     5161 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     5221 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     5281 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     5341 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     5401 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     5461 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     5521 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     5581 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatccta gttccccaaa
     5641 agggcaacca actggaacgt tttatctgta tggcattatt tgtctggtcg gtatggatgc
     5701 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaataatc aataccttgc
     5761 agatccagtt tcatcggctt attaagcatt aggttgaata ctaaaaagac aaaaatcacc
     5821 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     5881 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     5941 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6001 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     6061 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     6121 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     6181 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     6241 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     6301 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     6361 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatctga
     6421 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     6481 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     6541 tgaaactgct gaaggatgat gcgccgaaag gtgaacgcgt cttagccgct atcgataccc
     6601 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     6661 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     6721 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgcagtgcat ggcattctgc
     6781 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     6841 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     6901 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     6961 gtcagaacgg ctgcgccggt ttgtatccgg caatgctggc ggtgatggtt gcgcctacgg
     7021 ttggcattaa cccgctggac ccgatgtgga ttgcgacgct ggtcggtatt gttaccgtta
     7081 gttccgcagg cgttgccggt gtcggtggtg gtgcaacttt cgccgcactg attgtgctgc
     7141 ctgcgatggg cctgccagta accctggtgg cgctgttaat ctccgttgaa ccgcttatcg
     7201 acatgggccg tacggcgcta aacgttagtg gctcgatgac agctggcacg ctgaccagcc
     7261 agtggctgaa gcaaaccgat aaa
//
LOCUS       DAJTSL010000185         7203 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTSL010000185 DAJTSL010000000
VERSION     DAJTSL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.186"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(235..789)
                     /locus_tag="OO433_003622"
                     /pseudo
     CDS             complement(235..789)
                     /locus_tag="OO433_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            932..1054
                     /locus_tag="OO433_003623"
                     /pseudo
     CDS             932..1054
                     /locus_tag="OO433_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1316..1882)
                     /gene="ipgB2"
                     /locus_tag="OO433_003624"
     CDS             complement(1316..1882)
                     /gene="ipgB2"
                     /locus_tag="OO433_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6051956.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYHISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2164..2478)
                     /locus_tag="OO433_003625"
     CDS             complement(2164..2478)
                     /locus_tag="OO433_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051957.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSAQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2556..2666)
                     /locus_tag="OO433_003626"
                     /pseudo
     CDS             complement(2556..2666)
                     /locus_tag="OO433_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2990..3667)
                     /gene="ospD1"
                     /locus_tag="OO433_003627"
     CDS             complement(2990..3667)
                     /gene="ospD1"
                     /locus_tag="OO433_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6051958.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKRNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGVPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4038..4175)
                     /locus_tag="OO433_003628"
                     /pseudo
     CDS             complement(4038..4175)
                     /locus_tag="OO433_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4257..4555)
                     /locus_tag="OO433_003629"
                     /pseudo
     CDS             complement(4257..4555)
                     /locus_tag="OO433_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5144..5303)
                     /locus_tag="OO433_003630"
                     /pseudo
     CDS             complement(5144..5303)
                     /locus_tag="OO433_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5307..6461)
                     /locus_tag="OO433_003631"
                     /pseudo
     CDS             complement(5307..6461)
                     /locus_tag="OO433_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6481..6828)
                     /gene="tnpB"
                     /locus_tag="OO433_003632"
     CDS             complement(6481..6828)
                     /gene="tnpB"
                     /locus_tag="OO433_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6051959.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(6828..>7203)
                     /locus_tag="OO433_003633"
     CDS             complement(6828..>7203)
                     /locus_tag="OO433_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6051960.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLR
                     LKGKLTPALLQILIREIKGSSH"
BASE COUNT         1855 a         1678 c         1560 g         2110 t
ORIGIN      
        1 aaaccttctc atgtttagag ttttttatta tgtttatcat aatgatatga cgatctgagt
       61 ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac agtgcctgtg gataactttg
      121 cgtaaatccg gaggacagat cactctgaag caagacaaca gcatctgaaa acaatatgct
      181 cacgtttcaa ccacagggcg taagtgatcc tacccacgta atatggacac aggcctaagc
      241 gaggttctgg ttttcaaatt gttccggact gaggccgcca caccaactgt gccgtcgcca
      301 gcgattgtaa tcacattcga tataattaaa cactgttgcc cgcattattt cccggctgat
      361 aaagtgttct ccgtggagac attccacttt cagcgaatga aagaagcttt ccacgcaggc
      421 attatcgtag cagcagccct ttgcgctcat actgccacgc agattatgcc acttcagcag
      481 cgcctgataa tctgctgaac agtactgact tcctcggtcc gagtgaacga taacgttccg
      541 gggtctctta cgccgccaca gcgccatctg cagggcatcg caggccagtt gcgccgtcag
      601 gcgtggtgac atggaccagc caataacggc acgtgaccac aggtcaatga ccacagccag
      661 atacagccag ccttcatctg tacgtaagta cgtgatgtct cccgcccact tctggttcgg
      721 accactggcg taaaaatcct gctccagcag attttctgac acaggcaggc catgtgcgcg
      781 gtaactgacc gtattgctgg gtaagatcaa tttgctcact ggtatttact acgtgcatcg
      841 aatgagtgat caagagccgt atgacgcgag agtgtcacgt acggttccgt gagcagccgg
      901 agagtgaaac tctctccggc tgactcgact cgcaggttcc gacagcggtg acgaacatgc
      961 agcggtgttg ttctcgctga tcggcacatg ccgtctgaac aatgtggagc cagaaaaatg
     1021 gctgcgttac gttattgaac atatccagga ctgaccggca aaccgggtac gcgatctgtt
     1081 gccttggaaa gttgatctga cttctcagta aatatcaata tgattctgac gagccgctta
     1141 cctttgagca gagaaagggg agagggatcc cacatccatg agctaggaaa atacaaaagc
     1201 tggctataca acaattcaat atattaggac ttacttgcga ggagaaatat agcaccgctc
     1261 aggcaattcc tgggtattta cttttcttag tttctttact cgggatgttc tgtcatcaga
     1321 aaggcgattc taaatttgta atataatcac tcatattccc acgtaatttc tttagatcaa
     1381 tatattgcac atttttctca aaaaactgat cggaaataag atttgcaaca ttgcttgttg
     1441 tatcattttt aacgcttcta ttcatccctt cacaaagttc gtccattttc tttttgaaat
     1501 aactgcttcc tgatacaaga tgatttatgg aactttgtgc agcattgatg tctagagaaa
     1561 tgtgataatc tttctcaacc tgtttaaata tctgtttacg gagattatcg ccgatttttc
     1621 ggctgtgttc tgcacaaaac ttatcaatac attggtttac cacccgggat acgtagactt
     1681 ttttttcatc atttttccaa tctgtaatat tttcaccgat ttgttctgaa acagagctca
     1741 aaatactagt atttattttg gttgatgata tttttactat ccttttcccc attgtacagc
     1801 ggataatctc atcaaccttt cctgaaaaat atcttttgtg agataaactg attccaaaat
     1861 tattaaaaga tgttccaagc attataatca ctttgtcatg taaaatagta acattgtcat
     1921 tattttagct gattcaaaca attctgtaaa tgctttgagt tctttactcc cttggctcaa
     1981 tgatggaccg tctggaatcc cagagactaa actctctgag aaagagagta tgaaaaaact
     2041 tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat aaccggcatc attcataatg
     2101 atgccctgtt agttgtagtt atgaagattt acgtcattaa aattgctgtt cgtagtgtca
     2161 gtcctatggt ctggcatcag ctgaggatcg tagccgatac atcactggct tcacgacatt
     2221 cgtgttgagg ctattcatga aaactccacg ctgaaaacgc ctttttgtat aaacggtcac
     2281 ggtatgcccg gagccactgt tgctgatgaa ttcgataaaa ccttggcgtt tcttgaagct
     2341 atcgttaatg cggataatga aacaacgatt ggtgagattc gctcttttgc cgacaccctg
     2401 ggcgctgtcc ggttcaatcg caacaaaatc aaccggcagc tgagcaaacc caaccttgcc
     2461 tctctggcgc tggagcatga agtgatttag ctgggacgta gccactgaca gcttctataa
     2521 aagtatgatc ctacccacgt aatatgaaca caggcttaag tgaggttctg gttttcaaat
     2581 tgttcaggac tgagacctcc acaccaactg tgccgtcgtc agcgattgta atcgcacttg
     2641 atataattaa acactgtata tttcatttgt ggagtctcca gtctgagggg gctttatgca
     2701 ttccgtattg ggcactatga tgccagaaat ctgcaaggct ccacaccttc ctgtatttcc
     2761 cttcctctat cacagtgcac tttacttaat agggtgggat gtgttcatta catttattgt
     2821 gttttttatg attgtttttg cgtccagtgt cgttcgtcat gggaaactgg ttctatgggt
     2881 agtatcgctc agtctagtta actgtttgtt tctttttaga aattaagcag atcacacaat
     2941 tcggggagag ctccttctaa gtgcaattct ttgcagctat ttagcacact taaaacaact
     3001 cttgtatttt gtcagaaata atattaaaca tgtcttgttc attattgtta agtctgtctg
     3061 ctatttctgt aagttttatc ccactatgat ggcatatatt tatatatgcc atcatagcct
     3121 cgatattccc tttccctgcg gccagaaata atcctggtac cccctcatta tctcttacat
     3181 ccaatagttt aatcgtttct tttggtgata aatcaaaagt tttaataaaa tctccataag
     3241 cttgaatcgc agcggaatga ccttcttgaa gagctatgaa caaagcagat tccccagcat
     3301 tatttcgtgc tgcaagaact tctagttttt cctctttggg tatttcggct gccaacagcg
     3361 attcaccgta tttacgcgct gcatcagcat ttccatgcca aatcaatgaa aacaaattat
     3421 ggcggttata ctccttatta ttctttgtcg gaatggatat gctctgtatt ttggccaatt
     3481 ttctgaaaga aacaccacta tatccccccc ctgatttttc ttcattgatg gcgtgggata
     3541 tagcggtatt tgtcacgtta atgatattac gtttcattcc tttattttca ttagcagtat
     3601 tgctgcctat tattaggtgc atatttttgt tgtttgctgg atgtaatcca tagttattta
     3661 ttgacatttt tgttcatcct ttttgctaaa aaaaacaata acctattttt gtaatgtaat
     3721 agatattagt ctattgctaa tctaaaaaaa ccattgccac attgaatggc tatattcacc
     3781 aataaattct ttcgatttcg tatattgaca tatcgtagat atatcttatc cctaagattc
     3841 aataatcgag tccgtttacc attctgtgca agcgccttca ctcagaagtg accgtcaagg
     3901 cgatcaccaa acttgataat aaagcagctc attgccatac gccagtcctt aaggggcgtt
     3961 gtccatttct gtgatgctgc ctagatttaa ctgcctgctc cggcttatct acatctaaat
     4021 atgtgagcct aataccgcta cactttggcc agactatgtt tttctccggg aagtgggcta
     4081 accgtctcca taaagtgacg aacttttttc aacagtggcc atattgaacg gcactgatga
     4141 ttacgggtta ttgtctcatg aagagccggc cacatcactg acagcagtga cttcaggcct
     4201 tcagcaccag acagcctgaa ccagttaatc catcgcccga cagatttagt aatctagggc
     4261 tggtttttct tcagctgttg cggttcgaaa gtgccattac gatcacgcag agtacggatt
     4321 tccagaggtt catctccagt agtgataatc ttcgttaagc aaccattgcg ggaattgctg
     4381 ccagatctaa gctgattttt ctcgcaccca agatgatgag tcatattagc attgagtacg
     4441 gtttcatttt cagcatccaa tcaaactgac tgagatcttc aggatttttg agacttttgg
     4501 ctagttcatt agctaaagcc cgcaacttat tagatgtcaa ctgtttttca ttcataaatt
     4561 caccttcatt tgatatcaaa ttaaatagtt caacatcaga gaattataca aacttatgta
     4621 caggctccta tgatcttcga atcctgaaga ttatagtgaa aataagtttt cacgttgaaa
     4681 tcccacatag ttagtatatg ataaataatc atggatgacc tgattaacct cttggggtag
     4741 tgcttttata gaagaataaa tattctacta atttaataca tcacctaaaa tagtattgtt
     4801 ttttatggtt ggttgaaggt cgtggtgatg atgttagtcc ttttgagaat attccagtgc
     4861 cagatcttac ttataataaa ggggcttggc agtttggata aactattgtt cttattccac
     4921 taatacaaac tttttgtaaa gtatattaaa tatacttcat ttcatactga aatccccgga
     4981 agtattcaat caaacaatta caccataaaa ggaagagggg gccctaatta agttgtcaag
     5041 catgtttatg atacctgcag ggtataaatt ttctggctgc aagcagacac atataaaacg
     5101 tctttattga tcctacccac ataatatgga cacagtcgta agtgaggctc tggttttcaa
     5161 attgttccgg actgagacca ccacaccaac ggtgccgtcg ccagcgattg taatcgcact
     5221 cgatataact aaacactgtt gcccgcacta tttcccgatg gataatagtg ttctccgtgg
     5281 agacattcca ttttcagcga gtgaattttc atctgttgct gtatccagtc atacagtgac
     5341 tgcatcagtg gcgcggctct ggcttttctt gccgccagac gctgttctgc tgaacatccc
     5401 cggacttctg cctcgatggc atacagttca ccgatacgca gcagggcttc cgttattctg
     5461 ccggattcgt acaacgcccg gtaaccaccg taagcatcgg cctgaagcac tccgctgtaa
     5521 ccggccagat gattctgtgg atggatacct ttccggtccg gtgagtacgc gaaccagacc
     5581 gccggaggca tttccgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
     5641 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
     5701 ttaccgggca tcagcacata ctggcgcagg acgtcataca gcggctccag cagttcagca
     5761 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
     5821 atttctgact ggcggtataa cggcagatag cctgcatatt ttccagcgac aacatgggcc
     5881 agaagccccg ctccggcata actgcgtgca atgggttttg aaggcaccgg tgcctgcacg
     5941 atatggtcac accggcaaca ggccagtttc ggacgttgtg tttcgataac cttaaaggcg
     6001 ctgctgataa gctccagctg ctctgacaca tcacatccca gagaactgag ttcgccacca
     6061 caggccgggc agcattcctc ttccggacgg atgacccggg tttcacgggg aagtgaggca
     6121 ggtaacggtt tacgggctga agactggcgc aggggagatg gcagtaccgg gtcatattgc
     6181 tcacccagcg tttccgccat ttcttcctga agtgcgctga ttcgctcctg tgcttccagt
     6241 atctgccgtt cggtttttgc gcgaagtttt tctgagcttt aaccgaactg catgcgctgc
     6301 agtttcgcaa ccagcgcctt cagccggttg atttcggaag cataagccgc cacccgctgt
     6361 gagagcaggc ggttgtattt cgccatctgg cggatggtgt cctgttgcgt ctgcaacagt
     6421 gcccgcaggc gggcattctc atgagcaagt gaggtgtcca tatcctcact ttacaacggg
     6481 ttatatgcgg atgccagcgc gttccgttcg tttcgggtgt ttccagtcga tgccctcaag
     6541 aagcatggat aactgggccg gagtaaggtg caccttgccg tcacgggtga ccggccagac
     6601 gaagcggccc cgctccaggc gtctggtaaa gaggcacagt ccgtcactgt cagcccacaa
     6661 cacttttatc tggtcacccc ggcgtccgcg gaagatgaac agatgcccgg agaacgggtc
     6721 atccttcagg acgttctgaa cttttgaggc caggccgtta aagccatttc gcatatcggt
     6781 gatacctgca accagccaga tacgcgaacc tgcagggaaa gatatcatca gtggctgctc
     6841 ccttttattt cgcggataag tatctgtaat aacgccggcg tcagtttacc tttaagcctg
     6901 agagttccgg ccggcagaac cagctcacag cacagactgt cggacggtgt atttatctgc
     6961 tctggttcct gtgccggagc cgggatttta ttatccggct ccggcgttaa cgtcacggga
     7021 agcagtgcag gcatattttt tccggaaggc agcaggccac ctttccggta ttgatggcgc
     7081 cagttgaaga gcaggttatc gttgattccg ttttcacggg cgatctgcgc cacacaggct
     7141 ccgggctgca gtgactgctc cactaaggcg attttaaatt cataaggaaa attaggccgc
     7201 cgg
//
LOCUS       DAJTSL010000186         7122 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTSL010000186 DAJTSL010000000
VERSION     DAJTSL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.187"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..649
                     /locus_tag="OO433_003634"
     CDS             <1..649
                     /locus_tag="OO433_003634"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR6051961.1"
                     /translation="TYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
                     QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGH
                     AAFVDAGKLLPNIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAE
                     LLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            740..1987
                     /gene="tnaB"
                     /locus_tag="OO433_003635"
     CDS             740..1987
                     /gene="tnaB"
                     /locus_tag="OO433_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR6051962.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2119..3294
                     /gene="mdtL"
                     /locus_tag="OO433_003636"
     CDS             2119..3294
                     /gene="mdtL"
                     /locus_tag="OO433_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6051963.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            3269..4228
                     /gene="yidZ"
                     /locus_tag="OO433_003637"
     CDS             3269..4228
                     /gene="yidZ"
                     /locus_tag="OO433_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6051964.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            4385..5134
                     /locus_tag="OO433_003638"
     CDS             4385..5134
                     /locus_tag="OO433_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6051965.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5156..5722
                     /gene="yieF"
                     /locus_tag="OO433_003639"
                     /gene_synonym="chrR"
     CDS             5156..5722
                     /gene="yieF"
                     /locus_tag="OO433_003639"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6051966.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(5776..7113)
                     /gene="adeP"
                     /locus_tag="OO433_003640"
     CDS             complement(5776..7113)
                     /gene="adeP"
                     /locus_tag="OO433_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6051967.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1692 a         1823 c         1803 g         1804 t
ORIGIN      
        1 aacctacaaa tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtgccgat
       61 gggcggcttg ctgtgcatga aagacgacag cttctttgat gtgtacaccg agtgcagaac
      121 cctttgcgtg gtgcaggaag gcttcccgac atatggcggc ctggaaggcg gcgcgatgga
      181 gcgtctggcg gtaggtctgt atgacggcat gaatctcgac tggctggctt atcgtatcgc
      241 gcaggtacag tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg
      301 cggtcacgcg gcattcgttg atgccggtaa actgctgccg aatatcccgg cagaccagtt
      361 cccggcacag gcgctggcgt gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat
      421 tggctctttc ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga
      481 actgctgcgt ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat
      541 tgaagccttt aaacatgtga aagagaacgc ggcgaatatt aaaggattaa cctttaccta
      601 cgaaccaaaa gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactaca
      661 gagtggctat aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc
      721 ttcctctaaa ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg
      781 tgttatggtt atagcaggta cagtaattgg tggaggtatg tttgctttac ctgttgatct
      841 tgccggtgcc tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct
      901 tcattccggg ttattgttat tagaagcaaa tttaaattat cctgtcggct ccagttttaa
      961 caccatcacc aaagatttaa tcggtaacac ctggaacatt atcagcggta ttaccgttgc
     1021 cttcgttctc tatatcctca cttatgccta tatctctgct aatggtgcga tcattagtga
     1081 aacgatatca atgaatttgg gctatcacgc taatccacgt attgtcggga tctgcacagc
     1141 cattttcgtt gccagcgtat tgtggataag ttcgttagcc gccagtcgca ttacctcatt
     1201 gttcctcggg ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttccaggt
     1261 cgattactcc atcctgcgcg atgccaccag caccactgcg ggaacgtctt acttcccgta
     1321 tatctttatg gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag
     1381 cctgattatt tgctatggaa aacgcaaaga taagttaatc aaaagcgtgg tatttggttc
     1441 gctgctggcg ctggtgattt atctcttctg gctctattgc acgatgggga atattccgcg
     1501 cgaaagcttt aaggcgataa tctcctcagg cggcaacgtt gattcgctgg tgaaatcgtt
     1561 cctcggcacc aaacagcacg gcattatcga gttttgcctg ctggtgttct ctaacttagc
     1621 tgttgccagt tcgttctttg gtgtcacgct ggggttgttc gattatctgg cggacctgtt
     1681 taagattgat aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc
     1741 tgcgttgttg tatctgatct tcccgaacgg ctttatttac gggatcggcg gtgccgggct
     1801 gtgcgccacc atctgggcgg tcattattcc cgcagtgctt gcaatcaaag ctcgcaagaa
     1861 gtttcccaat cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct
     1921 ctttggtata accgtgattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt
     1981 tggctaaatc cttcaagaag ccagccattc gctggcttct tgcctctcag gaaatcactt
     2041 atgtccaaat ggcaactcgc ctgatcctcc ttcaccacgt atgctttgcg tcaccttact
     2101 atcaggacgc tttagcccat gtcccgcttt ttgatttgta gttttgccct ggttttactt
     2161 tatcccgccg ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat
     2221 gccagcgaag cgcagttgca tattgcgttc tccgtatatc tggcggggat ggcagctgcg
     2281 atgttatttg ccggtaaagt ggccgatcgt tcagggagaa agccggtcgc catacccggc
     2341 gcggcgctat ttattattgc ctcggtgttc tgttcactgg ctgaaaccag cacgttattt
     2401 cttgcaggcc gatttctaca ggggttgggc gcaggctgtt gttacgtagt ggcgttcgcc
     2461 attttgcgcg acacgctgga tgatcgacgt cgggctaaag tgctgtcatt actcaacggt
     2521 attacctgca tcattccggt gttagcgcca gtgctcggac atctgattat gcttaaattc
     2581 ccgtggcaga gtctgttctg ggcgatggca atgatgggca tcgcggtact gatgttgtct
     2641 ttgtttattt taaaagaaac gcgcccagcg tcccccgctg cttcggacaa accacgagaa
     2701 aatagcgagt cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc
     2761 gtttcggtga tcctcacttt cgtcaataca tcgccggtat tgctgatgga aatcatgggt
     2821 tttgagcgcg gagaatacgc caccattatg gcgttgaccg ctggcgtcag catgaccgtt
     2881 tcattctcca cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctcg
     2941 caggtgttat tccttgcagc ggggatcacc cttgccgttt caccttccca tgcggtttct
     3001 ctgtttggta tcacactgat ttgcgccggt ttctcggtag gttttggcgt agcgatgagt
     3061 caggcgttag gaccattttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg
     3121 caggtttgcg gttcgtcact gtggatttgg ctggcagcgg tggttggtat cggcgcatgg
     3181 aatatgctga tcgggattct gattgcctgt agcatagtga gcctgttgct gattatgttc
     3241 gtcgcgcctg gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa
     3301 tctgctgctc tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg
     3361 gatgaacgtg accccttcag cggtgagtaa gtcgctggca aagttaagag cgtggtttga
     3421 cgacccgctc tttgtgaact caccgctggg gctgtcgccc acaccgctaa tggtcagcat
     3481 ggagcaaaat ctggcggaat ggatgcaaat gagcaatctg ctgctggata aaccgcacca
     3541 ccagacaccg cgcggcctga agtttgagtt ggcggcggaa tcgccgctga tgatgatcat
     3601 gcttaatgcg ctgtcgaaac ggatctacca acgttacccg caggcgacca tcaaactacg
     3661 taactgggat tacgattcct tagatgccat tactcgtggt gaagtggata ttggtttttc
     3721 cggtcgcgaa agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga
     3781 ttatgaagtg ctgtttagtg atgtaccctg cgtctggtta cgcaaagatc atccggcact
     3841 gcatgaaacg tggaatctgg acaccttttt acgttatccg catatcagca tttgctggga
     3901 acagagcgac acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat
     3961 tgctatgagc ctgccggaat ttgagcagtc actgtttatg gctgcgcaac ccgacaatct
     4021 gctactggcg accgcgccgc gctactgtca gtactacaat caactccatc aactgccgtt
     4081 ggttgctctt cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag ttccttttac
     4141 cctgctgtgg cataaacgga acagtcataa tccgaagatc gtctggttac gggaaaccat
     4201 taaaaacctt tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc caatttcaca
     4261 ataaaatgta aaaaagttgt aataagcttg tctgaatcga acttttagcc gctttagtct
     4321 gtccatcatt ccagtaaatg attactctta tattcataat ggaccattaa gcatggagcg
     4381 aaaaatggcg actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg
     4441 tctcccctcc cagtgtcatc aagaagcccg aaacattccc cctcatcgtc aaagccgttt
     4501 tctggcgtcc agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt
     4561 gccggaaatc gtcaccctgc cgaaaggtaa accggttttc agtgataaaa atttgccttc
     4621 gttttctatt tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg
     4681 tggcctcgat atggaactac agcgtgcgac gcgcgggttt catagcccac acgcgcccga
     4741 taaccacacc ttttccagca atgaatcgct atggatcagt aaacaaaacg atcctaacga
     4801 agcgcgggcg cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt
     4861 gaatgacgat ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca
     4921 tgtaaatcat gtagaagcgt tatgcgacgc ggaggacgtg ctggtgtggt ccgtggcggt
     4981 cacgcccgcg attgaaaagc tcagtgtctg ggagttagat ggcaaacaca gctggaaaag
     5041 cctgccggat attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca
     5101 gctctctacc gtgaagtctt tttcgccaaa ttgatagaca accacaggag tcattatgtc
     5161 tgaaaaattg caggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat
     5221 ggttgcacgt accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc
     5281 cattgccgac attcccctgt atgacgctga cgtacagcag gaagaaggtt ttccagcaac
     5341 ggttgaagct ctggcggaac agatccgtca ggctgacggt gtggtgatcg tcacgccgga
     5401 atataactac tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc
     5461 ggatcaaccg ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg
     5521 cggcgcgcgc tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat
     5581 gaacaagccg gaatttatgg gcggcgtgat tcagaacaaa gttgatcggc aaaccggaga
     5641 agtgattgat cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt
     5701 tattcagcga gttaagatct aaataaaaaa cccgccagta atcatgcatg gcgggttttt
     5761 aatgcgctat cgattttaat gagcgtcgat aaatacaatc ttcaggataa acagcagcgc
     5821 aacgatgatt acgcacgggc taaggtcacg cagacgcccg gtaccaatct tcatcacgca
     5881 gtaggagata aagcccagcg caataccttc ggtaatcgaa aagctgaacg gcatcatcac
     5941 ggcggtaata aacgccggaa cagattcagt aagatcctgc cagttcacgc gagcaagact
     6001 tgaggtcatc aacacgccaa cgtagatcaa cgcgccagct gcagcgtagc ctggcaccat
     6061 tcccgccagc ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt
     6121 cagaccggta cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga
     6181 agaagtaccg ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt
     6241 catgcgcggg aatttcccct tctcatccgc cagacctgct ttatcggtca cgccaatcag
     6301 cgtaccggag gagtcaaaca agttgaccaa catgaaagag aaaatcaccc ctgccagccc
     6361 gaggttaaac gacccggcta aatctacatg acccacaact gtcattacgc tcggcggcgc
     6421 agaaacgatg ccattgtagt gcacatcacc cagcatccag cccagcagcg tcgtcaccac
     6481 gatagaaacc agcaccgctg cgtgaatgtt gcgcgaggcc agaatagcaa tgatgaagaa
     6541 gccgaggata cccagaagta cgctgtgaga agtcagatta ccgatgctca ccagcgtttc
     6601 cgagttagcg acaatcacac ctgcgttttt cagccccatc atgccgataa acaggccgat
     6661 accgctggta atacccacac gcagacttac cggaatgtta gcaatcatcc agtaacgaac
     6721 gcggaaaatg gtcaaaagaa gcagtccgac agcgccccag aagattgcgc ccataccgat
     6781 ctgccacggc agccccattg cctggacaac cacaaaagcg aaaaaggcgt taagccccat
     6841 cgcaggagcc agcgccaccg gaaggtttgc aaacaatccc atcataatgc taccgaaagc
     6901 agcaatcaga caggttgtta cgaagacggc actggtgtcc atgccagcaa cgcccagaat
     6961 ttgtgggtta acaaaaacga tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac
     7021 ttcggtccgt gccgtcgtgc catgttcgcg cagtttaaac acgcgttcca gcatcccctg
     7081 gccagaagcc tgggttgtgt gttgttgact cattatctat tt
//
LOCUS       DAJTSL010000187         7026 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTSL010000187 DAJTSL010000000
VERSION     DAJTSL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.188"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..247
                     /locus_tag="OO433_003641"
     CDS             <1..247
                     /locus_tag="OO433_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051968.1"
                     /translation="HARLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILN
                     TLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(216..1952)
                     /gene="aceK"
                     /locus_tag="OO433_003642"
     CDS             complement(216..1952)
                     /gene="aceK"
                     /locus_tag="OO433_003642"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR6051969.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     LAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASELWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2233..3530)
                     /gene="aceA"
                     /locus_tag="OO433_003643"
                     /pseudo
     CDS             complement(2233..3530)
                     /gene="aceA"
                     /locus_tag="OO433_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(3560..5161)
                     /gene="aceB"
                     /locus_tag="OO433_003644"
     CDS             complement(3560..5161)
                     /gene="aceB"
                     /locus_tag="OO433_003644"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR6051970.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASICDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFGDAA
                     RLMEQITTSDELIDFLTLPGYCLLA"
     gene            complement(5430..6359)
                     /gene="metA"
                     /locus_tag="OO433_003645"
     CDS             complement(5430..6359)
                     /gene="metA"
                     /locus_tag="OO433_003645"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6051971.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6516..6963
                     /locus_tag="OO433_003646"
                     /pseudo
     CDS             6516..6963
                     /locus_tag="OO433_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         1684 a         1807 c         1826 g         1709 t
ORIGIN      
        1 ccatgcacgc ctgtttggat ttaccgctga agatattatg gatttttggc aacacaaagc
       61 gccacaaaaa tactctgcct ttgagttggc ttttgaattt ggtcaccggg ttattgctga
      121 attaatcctt aatacattaa ataagatggc tgaaagcttt ggctttacgg ataaccctcg
      181 atacattgcg gagaaaaatt atatggaagc tttactcaaa aaagcatctc cccataccgt
      241 acgctaaatc tttgcctgcg ccgatacgca taaacatctt ccacatgccc ttcacgtatg
      301 cggttttgta gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat ctcttcaaac
      361 agcggaccaa tacgcgggtc ggcgcatagc cagtggcgaa actcttccgg gaaaacatcg
      421 cccggcgaga cgctgtacca cagttcgctg gcaagttcgt cttccggata gcgcggcggc
      481 gggatgtcgc ggaaattcac ttccgtcatg tagcaaattt catcgtaatc ataaaaaacc
      541 acacgcccgt gacgggtgac accgaagttt ttaaacagca tgtcgccagg aaaaatgtta
      601 gcagcggcaa gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg caactgctga
      661 ccttccactt gttccagcca gatattgagc ggcaccatcc gccgctcgat atagagatgg
      721 cgaattacaa tctgctcacc aagatcggtg attttttccg ctgcttcctg gagcagtaat
      781 tccattaatg ccggggaaat atgccgcttc tccagcacaa agttttcaaa ctcctgggtg
      841 tccgccattc ggcccacacg gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga
      901 gcggcagaca tctctttctg cggcgcgaac ttgtctttga tgactttgaa tacccgatca
      961 aagcccggca gcgtaaacac caacatcacc attccacgaa tacccggcgc ttcaatgaac
     1021 tgctcattac agccctgtag ataaacgaga tattcgcggt agctttcggt tttggcgtgc
     1081 ttctggcagc cgatagccat atacaattca gcagtggttt tacctggcag aatttcccgt
     1141 agccactcga ccagcgctgc gggcagcggt gcataaacca taaaataaga acgcgcaaag
     1201 ccaaaaacaa tgctcgcttc ggcggtcgtc gtcaggcagg tatcaataaa taactcgccg
     1261 tcgtccgtct ggtggatcgg cagtaagaag ggcaacgtgc cggaaggcgt gatcagtttg
     1321 cccaccagcc aggcggcttt attgcggtaa aacagttcgt tcgccacctg taaatgactt
     1381 tccgcgaggt tgtctgtccc cagcgtttcc gtcagatggc gaatgatgta atgaatgtca
     1441 cggcttttat tctgccagcg caggcgcagc ggcaggtcgc tgataacgcg catcagtaga
     1501 gattcccagc cgtgatcggg gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag
     1561 cggcgctctg gctgagagct aaagataaaa agccgctcgg gagtaagcga gcggtggtca
     1621 aataaccgac agtacacgga gttaaaaaag ctctccgcaa tctcgaagcg cgggtaatcc
     1681 ggcaacagcc gcgtgtaatg ctctttaacg cgcagtaaaa atgccgcgtc cgtactttgg
     1741 ccgttagtaa tgcagcgcag ttgctccacg accagaccaa cgtgatgatc gtaaagatgg
     1801 atacggtttt tcatcgccag ctggacagca tgccagtcgg cctgttcgaa acgctgctgc
     1861 gccccggaag tcacttcgag gaatcgacca tactgagcgt cgaagccttg caaaatggtt
     1921 tgagcaatca gtaattccag gccacgcggc atttagcgcc ctcatcagga gcagagaatt
     1981 gccggatgcg gcgtaaacgc cttatccagc ctacgttcgg caacggttgt aggcatgata
     2041 agacgcgcaa gcgtcgcatc aggcatcgtg caccgattgc cggatgcggc gtaaatgcct
     2101 tatccggcct acgttcggca acggctgtag gcatgataag acgcgcaagc gtcgcatcag
     2161 gcatcgtgca ccgattgccg gatgcggcgt gaacgcctta tccggcctac agtcagcaac
     2221 ggttgttgtt gcttagaact gcgattcttc agtggagccg gtcagcgcgg tgactgaaga
     2281 cgtgccgccc tgaataatag tcgtcacttt atcgaagtaa cctgtaccca cttcctgctg
     2341 gtgagatacg aaggtatagc catctttcgc ggcggcaaat tccggctgct gcactttctc
     2401 aacgtagtgc ttcataccct cgccctggtc ataggcgttt gccaggtcaa acatgttgaa
     2461 ccacatgctg tggatacctg ccagggtgat gaactggaac ttgtagccca tatccgacag
     2521 ctgctgctgg aagctggcaa tagttttgtc gtcgaggttt ttctgccagt tgaacgacgg
     2581 cgagcagtta taagccagca gtttgcccgg atatttcgcg tggatagctt gtgcaaagcg
     2641 acgcgccagt tccagatccg gcgtggaggt ttcacaccag accaggtcag catatggcgc
     2701 atacgccagg ccacggctga tcgcttgctc aatgcccgca tgagtacgga agaagccttc
     2761 actggtacgc tcgccggtaa taaattcgct gtcatacggg tcgcaatcgg aggtgatcag
     2821 atccgcggca tcggtacgag caaccagtag ggttggaacg cccgtcacgt cagctgccag
     2881 acgcgccgcg accagtttct gaatagcttc ttgagttggc actaaaactt tgccgcccat
     2941 gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg ctgcaccggc
     3001 ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac cggcttccgc
     3061 atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct caatgcccgc
     3121 ggaccattgg atctgatccg cacggcggaa agtattgttg atccgctcca ccacagctgg
     3181 caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca ggttagcgtc
     3241 cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg cctgttgcag
     3301 cgcctgaccg ccagtcagtg caccgaggct gttgatgtag cttttttcga ctcgccatgc
     3361 agcagacgcc acattctcgc cgcgcccagt tgcgccaggg tgcattcagg attgactgaa
     3421 tcgcgtaatt tcaccacatc ttccgcgctg tatgggcgag taatgccttc ccaacgcggt
     3481 tgagtccact ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat gtgcagatgc
     3541 tccatagtta tgtggtggtt tacgctaaca ggcagtagcc tggcagggtc aggaaatcaa
     3601 ttaactcatc ggaagtggtg atctgttcca tcaagcgtgc ggcatcgcca aaacgcccct
     3661 gggagaaacg ttcttcgccc agttcgctgg caatgacttt catctcttcg cccagcatct
     3721 ggcggaacaa ggctttagtt accggtttgc cattgctcaa cattttttga tgatggatcc
     3781 actgccagat tgaggtacgg gaaatttcag ccgtcgccgc atcttccatc aggccataaa
     3841 tcggcacaca gccgttgcca gagatccacg cttcgatgta ctgcacggct acgcgaatat
     3901 ttgcgcgcat accttcttcg gtgcgttcac cgtcacaagg tgccagtagc tgatcggcag
     3961 taatcggcgc gtcttgttcg cgcatcactt caagctgatt tttacgggag ccgagaatgt
     4021 cgttgaatac cgccattgcc gtatccgcaa ggcctgggtg agcgatccat gtgccatcgt
     4081 gaccgttatt ggcttccagc gatttatccg cttttacttt gttgagcacc tggttattgt
     4141 gctcttcatc tttgctcgga ataaacgccg ccatgccgcc catcgcaaaa gcaccgcgtt
     4201 tatggcaggt tttaatcaac aggcgtgagt aagcattcag gaatggttta tccatcgtca
     4261 ctgcctgtct gtctggcagg acgcgatcgg gatagttttt caacgttttg atatagctga
     4321 agatgtaatc ccaacgaccg cagttcagac caacaatatg gtcacgcagc gcgtgaagga
     4381 tttcatccat ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc ttgatggtgc
     4441 cgcgcggcag attaaagcga tcttctgcat agctgaagac ttcgctccac caggccgctt
     4501 cctgccagga ctgggttttc ggcagataga aatagggacc actgcccttt gccaacagag
     4561 cctgatagtt gtggaagaaa tagagcgcaa aatcaaacag gctgccgggg attgcctcac
     4621 cacgccaggt gacatgtttt tccggcaagt gcagaccgcg tacccgacaa atcaaaaccg
     4681 ctggattggg cttgagctgg taaattttgc ctgcttcatt ggtatagctg atggtaccgt
     4741 taactgcatc acgcaggtta atttgcccgt cgatcacttt gttccagtcc ggggccagtg
     4801 aatcttcgaa atcggccata aagactttca cattggcgtt gagcgcgttg atcaccatct
     4861 tgcgctctac cgggccagtt atctctacgc ggcggtcttc taagtccgca ggaatcccgc
     4921 gaattttcca atcagcatcg caaatggaag ctgtttccga aataaaatca ggcaacgttc
     4981 cgttatcaat atcttgctgc tgctgaatgc gcgctgccag aagtttattt cgttgtggcg
     5041 taaaatgcgt caccagctca gtcagaaatt ctaccgcttc ggcagtaaga atttgcttct
     5101 cctgctcgcc atacggcctt gtgaaagcca gttcatcggt tgttgttgcc tgttcagtca
     5161 tcgtgcagct cctcgtcatg gatccgaaaa cttccccact gtgaacgaag gatcgttgtg
     5221 cgcttttcgt tcagcacaac taagactact catttaaaat gcaaaatcaa aaacaatttc
     5281 cattttaatt gaaaatgctt gataacttat tgataacaat gtgaataaaa tttattcacc
     5341 tcctgaggtg cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt aaggtgctga
     5401 atcgcttaac gatcgactat cacagaagat taatccagcg ttggattcat gtgccgtaga
     5461 tcgtatggcg tgatctgata gacgtaatag ttgagccagt tggtaaacag caaattaccg
     5521 tgactacgcc agctcgctcg cggtgtattt tgcggatcat tgtgcgggaa atagttatac
     5581 ggtacttccg ggtctagtcc ggcttccaca tcgcggaaat attcctgcgc cagcgtttgc
     5641 gcatcatatt cgggatggcc cgtcacaaag gcaatgcgct tatctttgct ggcaaacaga
     5701 tatgcatccc cttcttccgt ctctgccaga atttccagat cggtgtaatc acgaatcaac
     5761 gctgccggaa agtcagcata gcgcgaatgt ggtgccagga atgaatcatc aaagccacgc
     5821 gtcagaagcg catgaggatg gagaatatga tgctcgtaaa cgccagagag tttgtcggtg
     5881 cgagtttgct taggaatgcc gtagaggata ttgagcgcgg cctgtaccgc ccagcagaca
     5941 aacagcgtcg aggtgacgtg atctttcgac cactccagca cctgtttgat ctgcggccag
     6001 taagcgacat cattaaactc caccaggccc agcggcgcac cggttacaat cagaccgtca
     6061 aagttctgtt cctgaatatc ttcaaagtta cagtagaagt tgttcagatg ctctgcgggc
     6121 gtgttgcgcg attcacggga atcgatgcgc aacagctgaa tatcgacctg caaaggtgag
     6181 tttgaaagca ggcgcagaaa ctgattttca gtttcaatct tcttcggcat caggttaagg
     6241 atcagaacct taagtggacg aatttcctga ccagacgcac gagaagttgt catcacaaag
     6301 acgttttctt cacgcaagaa attgacggcg ggtagctcgt ccggcacacg aatcggcata
     6361 acctgattac ctcactacat acacttatac gtttagacat ccagatagct gaagataacc
     6421 agaaaatttg ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa ggaaaagact
     6481 acgttagaat ataagaatga ctaaaaggag agaaaatggt tattagtatt cgccgctcac
     6541 ggcatgagga aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat gccacacacg
     6601 attttttatc agcagagtat cgggccgagc tggaggtgct agctagttcg ttccttcctg
     6661 ccggaagcgc cgttgtggat cgcggttaat gaacgggatc agccggttgg gtttatgttg
     6721 ttaagtgggc agcatatgga tgcgctgttt atcgatcctg atgtgtgcgg ctgcggcgtg
     6781 ggtcggatgc tggtggagca tgcgctctcg atggcaccgg aactgacaac caacgttaat
     6841 gagcaaaatg agcaggcggt tgggttctat aagaaggtgg gttttaaggt tacgggacgc
     6901 tctgaggtgg acgatttggg gaaaccgtat ccgttgctga atctggcgta cgtgggggaa
     6961 taagccgccc ctgccgtgcc tgcccaggct ggagaagacg cgccagcgtc gcacccggca
     7021 tttttt
//
LOCUS       DAJTSL010000188         6948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTSL010000188 DAJTSL010000000
VERSION     DAJTSL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.189"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(559..1089)
                     /locus_tag="OO433_003647"
                     /pseudo
     CDS             complement(559..1089)
                     /locus_tag="OO433_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(1432..2082)
                     /gene="zinT"
                     /locus_tag="OO433_003648"
     CDS             complement(1432..2082)
                     /gene="zinT"
                     /locus_tag="OO433_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6051972.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2339..2974)
                     /gene="msrQ"
                     /locus_tag="OO433_003649"
     CDS             complement(2339..2974)
                     /gene="msrQ"
                     /locus_tag="OO433_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6051973.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(2975..3979)
                     /gene="msrP"
                     /locus_tag="OO433_003650"
     CDS             complement(2975..3979)
                     /gene="msrP"
                     /locus_tag="OO433_003650"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR6051974.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4088..4501)
                     /gene="hiuH"
                     /locus_tag="OO433_003651"
     CDS             complement(4088..4501)
                     /gene="hiuH"
                     /locus_tag="OO433_003651"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6051975.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4634..5305
                     /gene="hprR"
                     /locus_tag="OO433_003652"
     CDS             4634..5305
                     /gene="hprR"
                     /locus_tag="OO433_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6051976.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5305..6663
                     /gene="hprS"
                     /locus_tag="OO433_003653"
     CDS             5305..6663
                     /gene="hprS"
                     /locus_tag="OO433_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6051977.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASLGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSASYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(6771..>6948)
                     /locus_tag="OO433_003654"
     CDS             complement(6771..>6948)
                     /locus_tag="OO433_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6051978.1"
                     /translation="HLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANAL
                     GKLAAQEMLAAYAG"
BASE COUNT         1954 a         1434 c         1577 g         1983 t
ORIGIN      
        1 acttatctcc cgacttcccg ccttactttt cctaaacaga acaatcgtag aatattcttg
       61 aagggttaga tcgtcactgt tttctgttcg atactgtgac attcagcact tgattcgcta
      121 tggatctgac aggaagcttt cgagcgaaaa tgtgcagtta ttcagtcgtt ttcttatcgc
      181 tcaccattat tcttttagac attcatccta tgaaactgcc gcaaagttgg tggtgggaac
      241 tgaagttgcg tagagaaggg gtcaataatt gagtcaaaaa aggaacgcgg ccgtcagatt
      301 aaagccgcgg gacaaagtcc ataatttcca agagttgttg aatttcccag cttttttgaa
      361 agcctttgta tcatactaac aagtaaaaaa ggtttaaata ccaaaattaa cctgtatttt
      421 tgataagtct taccattgta cgatttacac agtttctgct cctccttgtt gatagtggaa
      481 agggtgcatt gtgcacttgc gtaaacattt atgacgcgtt gatgtccatg gttcacattt
      541 ccttgtcagg ggatcgactt atttgcggcg aggcatcatt cgtaacagag tattatcttt
      601 ctgaatgtag tgatgaaaga gtgctgcgcc agcgtgagct gcgattaaaa agtaacctat
      661 atttgcccag gtttcgtgaa tattttatat cagtgcttta atttcgctgt ttggggtaac
      721 aaagggagac acattgaaac caaggaaact ccacgatttt ccactgtaag ccatcaaagc
      781 aatccccagc agaggaagtg caaggaaggt tatataaagc atgatgtgca ttagcgaagc
      841 cgctttcatt tgccaggcag gtggcggtgg cacaatagaa ggatcactat aacggtgttt
      901 tataatcagg cgtgaaaaca ttaacaccca aacgaatata cccgcattgt aatgtgtttc
      961 tcgcatcagc agataagtac tactaccttt aggaaaccag ccacggagtt ccatcgcggc
     1021 ataggttatt gcaacgaaaa gcagcgttat ccaatgtaaa taaatttgag tttttgagaa
     1081 tcggttcatt tttgtagctc ctgtaatttt tacagtcaag ctatcccacg aaccttaaaa
     1141 attccttata cagatgtggt tttaagaatt tgttatttaa aaaattgata gtgcacattg
     1201 taaccagcca atataagcaa tggcgtgagc acaggtatct ttctctgttg gccgtattgt
     1261 gtatggaatc cgttattggt taactcaatg cgggttagct tattaatata cagacacgtc
     1321 atagtgatga gcgtgtctgt agctagcttg cgttcagtgg cgtttgtgga tggaccagtg
     1381 gcagttatga tgacttacta aagcggcatc gaggcgttat catgagaata ctcaatgaga
     1441 catcatttcc tcgaccactt cttcgctact caactgatat ggataatacg ttggccagtt
     1501 ttccatttca tttaataaag attgctgtga atcattaccc ataaaaatgt gaaaatgaga
     1561 tgattttcgt ggtgcaatta tatgatcgct aaattgtata tatttagggg ctttgctttc
     1621 aggatcttta cattcgaata agtaacgaac gcctttcttg cctgatttat aggtgagtat
     1681 tttgtatcca tcgtaatcat atttacagga tgttgtttca ttatttctat ggaattcaac
     1741 aatgccgtcc tcaatgccaa tcatctcgat atctgttgca taacctttgt gataataatc
     1801 tttaatttca gcaaatgttt tagttttatc tgcatccgct ttcttctgaa agacggggtc
     1861 aagtttgcca ctttgcagta aaggataaac ggattgccag accccatccc agtcactgag
     1921 cgttcggttt tgtacattgg catcatcaaa aacaccatta gcagcttttt gttcgacctc
     1981 tgttaagggt ttgccgtgtg agtgatgacc atgcgaaaag gcaggagcgc taacaataaa
     2041 gacacctaac gcaacagcca gtttgtgaag acgaatcgcc aaaacagttt cctccagagt
     2101 cattgtatat gatgtgtaat gttatattgt aacatatggc aaaatgcaac aactcttagg
     2161 agatgagagc atagttttct caggtgtact tatctatctt attgaaataa aatgaattat
     2221 attgataatt tgaggttgat gacaaacgaa acataatctg atgtgatctg cgtaggccgg
     2281 atagggcgct cgcgccgcat ccggcaggga taacgcgtac tttgtcggca atatgaagtt
     2341 atacagataa tttgttgtga acctgcttgc gtaaccggtt aaaaagggaa cgcaacttct
     2401 tataccgtaa ggctaaaagc agtacagcca gcccagcgta gatgagcggc tgcggtgaaa
     2461 taatcttcac tgaccacaga taatgtattg gggccaggat cgcgacaaga tagacgaaat
     2521 tatgcaactg ttgccagtgc ttgccgagtt ttcgctgcat cgcctgggtt gaagtaaacg
     2581 ctaaagcaag caaaataacc cagctgataa ttcctaacgt taaataaggc cgggtaatta
     2641 actccttgcc taataacgcc agattattca cgcccagctc cagcaatgcg taactggtta
     2701 aatgcagtgt cgcccaggca aagcaccata agcctaacag gcggcgagtg cgtatcaata
     2761 acggctgttt tgcgtagcgt gccagaggtg tgattaacaa ggtcgccagc aaaaatttca
     2821 gtgcagtgcg accggtaaaa tgctgaatat ctttcaccgg atcggcaccc agtccaccgt
     2881 gattgatcgc ccagaccagc cagagaaacg gcaacaatcc ggcaagatgc aggctaactt
     2941 tcagccatgt cacctgtttt gctgtcagac gcattcagaa attctcccgc aaatccaggc
     3001 cacgatacag cgatgccacc tggtcggcgt aaccattaaa cagtagcgtt ggctggcgct
     3061 gaacatcgag gatgccgcct gaaccaataa atcgttcggt agcctgtgac cagcgcgggt
     3121 gatcaacatg cggattaacg ttggcgtaaa aaccgtattc gtcaggcgct gccagattcc
     3181 aggtggttgg cggacgctcg cgggtcagct taatactgac tatcgattta atccctttaa
     3241 agccatattt ccacggcaca atcagtcgta ccggcgcgcc attttgtggc ggtaacgcct
     3301 tgccataaac acccacggtc atcagtgtga gcggatgcat tgcttcgtcg agacgcaatc
     3361 cttcgacata aggatatttc agcccgccgc cgataaagcg gtcctgctgg cctggcattt
     3421 gttccggtgc ataaattgtt tcgaaagcga catacttcgc attgctggtg ggttcggcaa
     3481 gcgccagcaa tttgtgcagc ggaaaaccaa tccacggcac caccatcgac catgcctcca
     3541 cgcagcgcat acgataaata cgctcttcca gcgggaagcg acgggttaaa tcatcgtgat
     3601 cgagggtcaa tggttttgcc acttcgccgc tgattttcag tgtccatgga tcggttttca
     3661 ggctaccagc attagcggcg ggatcggctt tatccagccc gaattcatag aagttgttat
     3721 aaccggagac tttatctact ggcgtcagtg gcaggttatt ttgccaggcg gcaggcttgc
     3781 tgaactccag cggttttccg gcgggggcgg gcgggcgatc gttcccttta aaccagctaa
     3841 gcagatcggc atgcgcagcg tgaggcaaag aaagtgcagc tgcactgatg cccagtgctt
     3901 ttaacacctg ccgacgcttc ataaagaata ccgactcggc cgtaacatct gattctttta
     3961 aaaattgatt ctttttcata agccatcaca cttttttgtc gttagccagc aagagccgtt
     4021 tactgttaat ctgtcaggga gtgtcctccc taacagtagc agagatctgc ttttacatat
     4081 ttatgcatta actgccacga taggttgaat acccatattg gctaagtaat aaaggcacat
     4141 gataatgctc gttcacttta ttaatatgaa attcaacggg gatctcaggg aagaaacttt
     4201 caagattttg tttcttgaaa tagtccccgg ttttaaatac gacacggtaa tcgcccgtag
     4261 ttgcagtttg ctcgggccac agtgctttaa ttcgtccatc cttatctgtt ttggcggtat
     4321 taagttgtaa ccagccgtta tccgccttct tttcaagagt gactgtcacg tcagcagcgg
     4381 gctttccggt ttgctggttc aaaatgtgca cgctaagaat gttttgttgt gcggcataaa
     4441 ccaaagaagg taatgaaaat gctgccgttg ctaccgagag tactaaataa cgctttaaca
     4501 tgtttatatc cttgtcatgt gaatgagttt tgaggatagc gaaaaagcgg ccagggtaac
     4561 cttacagcag cattacaatt ttgtaatgaa ccaggctgtt tctataacat atgatttatg
     4621 gcatattatt ttcatgaaga ttttacttat tgaagataat caaaggaccc aggagtgggt
     4681 aacgcagggg ctttccgaag cgggttatgt cattgatgcc gtttctgatg gcagagatgg
     4741 gctttatctt gcgctgaagg atgattatgc attgataatt ctggatatta tgcttccggg
     4801 tatggatggc tggcagatct tacaaacgtt aagaacagca aagcaaaccc ctgttatttg
     4861 ccttactgca agggattctg tcgatgacag agtcagaggg ctggacagtg gagcaaatga
     4921 ttatctggta aaaccttttt cattttctga gttgctggca agggttcggg cacaattaag
     4981 gcaacatcac gctttgaatt caacattaga aatcagtggc ttaagaatgg actctgtaag
     5041 tcaaagtgtg agcagggaca atatcagtat tacactgacg cgcaaggagt ttcagttact
     5101 ttggctactg gcctccagag ctggcgaaat tatacccaga acggttattg cgagtgaaat
     5161 ttggggaatc aactttgata gtgataccaa tacggtggat gtcgccattc gcaggctccg
     5221 cgcaaaagtt gatgatcctt ttcctgaaaa gctaatcgcc acaatccggg ggatgggcta
     5281 ttcattcgta gcggtaaaaa aataatgaaa agattgtcta taaccgtccg tttaaccttg
     5341 ctttttatat tgctgctgtc tgttgctggc gctggaattg tctggactct ctataatggc
     5401 ctggcaagtg agttgaaatg gcgcgatgat acaacactca ttaaccggac agcacagatc
     5461 aagcagttgt taattgatgg ggtaaatcca gatacgttac ctgtatactt taaccggatg
     5521 atggatgtta gtcaggatat cttgatcatt catggtgatg gcatcaataa aattgttaac
     5581 cggacaaatg tcagtgatga catgttaaat aacatacctg ctagtgagac aatcagcgca
     5641 gctggcattt acagaagcat tattaatgat actgagattg atgctttacg aattaatatt
     5701 gatgaagttt cgccatcatt aacggttact gtggctaaat tggcttcagc tagacataac
     5761 atgcttgaac agtataaaat taatagcatt ataatttgca ttgtcgccat tgtactttgc
     5821 tcagtattaa gtccgctgtt aatcagaacg ggattacgag agatcaaaaa gttgagtggt
     5881 gtaacggaag cgctgaatta taacgatagc cgagagcctg ttgaggttag cgcattaccg
     5941 agagaactaa aacctcttgg gcaggcgttg aataaaatgc atcaagcctt agtcaaagat
     6001 tttgaacgcc taagtcaatt tgctgacgat ctcgctcatg aacttagaac gcccattaat
     6061 gcattactgg gtcagaatca ggttacgctc agtcaaacca gaagtatcgc tgaatatcaa
     6121 aaaacaattg ccggtaacat tgaagagttg gaaaatattt cgcggttaac agagaacata
     6181 ctgtttcttg cccgggcaga taaaaacaat gttttggtga aactggactc gctttctctc
     6241 aataaggaag tcgaaaattt gttggattat cttgaatatc tttcagacga gaaagagatt
     6301 tgctttaagg tcgagtgcaa tcagcaaatc tttgcggata aaattttact gcaacgaatg
     6361 ttatcgaatc ttattgttaa tgccattaga tattcaccag aaaaatcgcg tattcatata
     6421 accagttttc ttgataccaa cggctatctt aatattgata tcgccagtct tggaacgaaa
     6481 atacatgagc ctgaaaaact cttccgtaga ttttggcggg gagataattc gcgtcattcc
     6541 gtaggtcagg gacttggtct ttctttagtc aaagcgattg ccgaattgca tgggggaagt
     6601 gcttcatatc actatctcaa taagcataat gtgttccgaa ttacgttacc gcaaagaaat
     6661 taacataggg cttcagtacg ccataaatta accgtgaaaa aacgcagcca cgtcgtgagt
     6721 actaacgcgg ctgcgattaa ttatgcgctt acattcaaac gtaacaggga ttaacccgcg
     6781 taagctgcca gcatttcctg cgccgccagt ttacccaacg cattcgctgc gaaaggactg
     6841 tcgccggtga gaagtttacg gtccttatgt actcgcccgg tgatgtcgtc attaatgatg
     6901 ttcatgccca ttttcttcag ctcttcgccg aagtaccagg tgagatga
//
LOCUS       DAJTSL010000189         6930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTSL010000189 DAJTSL010000000
VERSION     DAJTSL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.190"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..669
                     /locus_tag="OO433_003655"
     CDS             <1..669
                     /locus_tag="OO433_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6051979.1"
                     /translation="DGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPF
                     WMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFN
                     VLSYLSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDM
                     NWQDVSGKSAANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIW
                     AGQQ"
     gene            847..2100
                     /gene="avtA"
                     /locus_tag="OO433_003656"
     CDS             847..2100
                     /gene="avtA"
                     /locus_tag="OO433_003656"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6051980.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNVFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(2251..2724)
                     /locus_tag="OO433_003657"
     CDS             complement(2251..2724)
                     /locus_tag="OO433_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6051981.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(2826..3674)
                     /gene="yiaJ"
                     /locus_tag="OO433_003658"
     CDS             complement(2826..3674)
                     /gene="yiaJ"
                     /locus_tag="OO433_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6051982.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            3875..4873
                     /gene="yiaK"
                     /locus_tag="OO433_003659"
     CDS             3875..4873
                     /gene="yiaK"
                     /locus_tag="OO433_003659"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6051983.1"
                     /translation="MKVTFEQLKAAFNRALISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            4885..5353
                     /locus_tag="OO433_003660"
                     /pseudo
     CDS             4885..5353
                     /locus_tag="OO433_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
     gene            5471..5944
                     /gene="yiaM"
                     /locus_tag="OO433_003661"
     CDS             5471..5944
                     /gene="yiaM"
                     /locus_tag="OO433_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HCR6051984.1"
                     /translation="MKKILEVILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            5947..>6930
                     /locus_tag="OO433_003662"
     CDS             5947..>6930
                     /locus_tag="OO433_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6051985.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPDMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSTWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGM"
BASE COUNT         1648 a         1783 c         1755 g         1744 t
ORIGIN      
        1 gatggttttc gggtcgatac cgccaaacat gttgagttgc ccgcctggca gcaactgaaa
       61 accgaagcca gctccgcgct tcgcgaatgg aaaaaaacta accccgacaa agcattagat
      121 gacaaacctt tctggatgac cggtgaagcc tggggccacg gcgtgatgca aagtgactac
      181 tatcgccacg gcttcgatgc gatgatcaat ttcgattatc aggagcaggc ggcgaaagca
      241 gtcgattgtc tggcgcagat ggatacgacc tggcagcaaa tggcggagaa attgcagggt
      301 ttcaacgtgt tgagctacct ctcgtcgcat gatacccgtc tgttccgtga agggggcgac
      361 aaagcagcag agttattact attagcgcca ggcgcggtac aaatctttta tggcgatgaa
      421 tcctcgcgtc cgttcggtcc tacaggttct gatccgctgc aaggtacacg ttcggatatg
      481 aactggcagg atgttagcgg taaatctgcc gccaacgtcg cgcactggca gaaaatcagc
      541 cagttccgcg cccgccatcc cgcaattggc gcgggcaaac aaacgacact ttcgctgaag
      601 cagggctacg gctttgttcg tgagcatggc gacgataaag tgctggtcat ctgggctggg
      661 caacagtgac ttttccggct tcccgttcat cagtacctcg ggaagccgcc aaccaggata
      721 aaatgtcagc cctaatcagc gttgcaggat aaagcaccgc tcactcttca acagaccgat
      781 ttgcacccca gcaaatgtag cgttattgtt accttccttg ctacagagtt cgacagatat
      841 cccgctatga cattctccct ttttggtgac aaatttaccc gccactccgg cattacgctg
      901 ctgatggaag atctgaacga cggtttacgc acgcctggcg cgattatgct cggcggcggt
      961 aatccggcgc agatcccgga aatgcaggac tacttccaga cgctactgac cgacatgctg
     1021 gaaagtggca aagcgactga tgcactgtgt aactacgacg gtccacaggg gaaaacggag
     1081 ctactcacac tgcttgccgg aatgctgcgc gagaagttgg gttgggatat cgaaccacag
     1141 aatattgcac taacaaacgg cagccagagc gcgtttttct acttatttaa cctgtttgcc
     1201 ggacgccgtg ccgatggtcg ggtcaaaaaa gtgctgttcc cgctggcacc ggaatacatt
     1261 ggctacgctg acgccggact ggaagaagat ctgtttgtct ctgcgcgtcc gaatattgaa
     1321 ctgctgccgg aaggccagtt taaataccac gtcgattttg agcatctgca tattggcgaa
     1381 gaaaccggga tgatttgcgt ctcccggccg acgaatccaa caggcaatgt gattactgac
     1441 gaagagttgc tgaagcttga cgcgctggcg aatcaacacg gcattccgct ggtgattgat
     1501 aacgcttatg gcgtcccgtt cccgggtatc atcttcagtg aagcgcgccc gctatggaat
     1561 ccgaatatcg tgctgtgcat gagtctttcc aagctggggc tgcctggctc ccgctgcggc
     1621 attatcatcg ccaatgaaaa aatcatcact gccatcacca atatgaacgg cattatcagc
     1681 ctggcacctg gcggtattgg tccggcgatg atgtgtgaaa tgattaagcg taacgatctg
     1741 ctgcgcctgt ctgaaacagt catcaaaccg ttttactacc agcgtgttca ggaaactatc
     1801 gccatcattc gccgctattt accggaaaat cgctgcctga ttcataaacc ggaaggagcc
     1861 attttcctct ggctatggtt taaggatttg cccattacga ccgagcagct ctatcagcgc
     1921 ctgaaagcac gcggcgtgct gatggtgccg gggcacaacg tcttcccggg actggataaa
     1981 ccgtggccgc atacgcatca atgtatgcgc atgaactacg taccagagcc ggagaaaatt
     2041 gaggcggggg tgaagattct ggcggaagag atagaaagag cctgggctga aagtcactaa
     2101 aaattgcctg atgcgctacg cttatcaggc ctacatgatt tctgcaatat attgaatttg
     2161 gaagaatttg taggccggat aaggcgttta cgccgcatct ggcataaaca aagcgcacta
     2221 ttccggcgtt gagaaacgcc ggaaaacgtt tcaaacaggc tgctgccgta gccgatgccg
     2281 ctgcactttc tcgacatcca tgcactgcaa cgcctttgtc gggcaggctt caacacaggc
     2341 cggccccgtc tcccgatgcc agcacagatc gcacttaatc gccctcgcct ttttgcgcga
     2401 agaaacgacc tccatcgcac caaacgggca agccagcata cagcttttac agccaatgca
     2461 acgtgtttgt tcaacgaaaa tatgcccatg ttcgcggctt atcgcgtcaa cagggcagac
     2521 attcgcgcac ggtgcatctt cacactgatg acaggctact gccgtggtcc agctgtggtc
     2581 tttaatgaca cgaatacggg aaataaactc gtctggtgac aacgcagcgc aatcctgatt
     2641 ctcctgatgc gacactgcgc aagccacttc acaggtacgg caaccgatac atttcgtcgc
     2701 atccgcaata ataaaccggt tcatgatcac tcctttgcac taaaacagca gagtgccaga
     2761 gagcgcgtcc ctactgcttt gatccaacga aggaaatgtt aaagttttgg cacttgtcca
     2821 aagcgttatg tgatcgcgcc caggtcatcc ctgacagtaa atcccagttc attagaaata
     2881 gcctgcgcgg tttcacgcag tggtttcagg agatttttct ctcccacctg tttcagacgt
     2941 gatgtcgaaa gcgaaatcga cacggcgtac ggcacccgcc catgaatatc aaacaccgga
     3001 acagcaatac aggagacgcc gagttcgttt tcttctctgt ccatcgccgc tccgctttca
     3061 cgaatgtgcg ccagttcgtc gaacatcgcg ggcagctcgg taatggtatt gcgggttaac
     3121 ggctggatct catgctgatg gttttcccag tatgacttca cgtagtccgg gtgaccaaac
     3181 gccatgtaga tcttgcccat tgcggaacag tagagcggca tatgctggcc aatataggca
     3241 cgggttcgca gcatcccggt tgtgggttcc agcttataaa tcaaaatagc gtgatcgtct
     3301 tcgcggctgg agaagttaat ggtttcacca gtggcgatgt tcagtgcctc aagatgcgga
     3361 gcggcgatat gaatgatatt cagcgaagac agcgccttct gcccgacggc aataaatttg
     3421 gtagtcaggc ggtaactccc tgcggcgggc gcggtggtca catagccgca ggactgtaat
     3481 ccctgcaata agcgatggac ggtactctta tttaaaccag ccagctccga aagatgcgcc
     3541 aacggacaac cgtttggata gttgctcaaa atctcaatca gcatcaaccc gcgaaacaga
     3601 ctctggcttc cggctggacg ctctttttcc tgcgccatct cgttctcttt ttttcccatc
     3661 acttctttcc ccattttgtc gcgtcctgat ggtagcgcaa agtgtgccgt agttcacgat
     3721 ctcgacagat aatttataac cacttgattt ttatgtcttt tgaaattcat caatcagatt
     3781 gctttgttaa aaagtgatcg atatatttga aatcaagttt cgcatattga aattttaagc
     3841 caaaaaagcg atcaaaaaaa caaggaagcc tgggatgaaa gtgacatttg agcagttaaa
     3901 agcagccttt aatcgggcct taatttcacg cggcgttgac agcgaaacgg ctgacgcctg
     3961 tgcagagatg ttcgcccgca ccaccgaatc cggcgtttat tctcacggcg ttaatcgttt
     4021 ccctcgtttc attcaacaac tggaaaacgg cgatatcatt cctgatgccc aacccaaacg
     4081 tataaccagc ctcggcgcaa ttgaacagtg ggatgcccag cgttcgatcg gcaacctgac
     4141 ggcgaaaaag atgatggatc gcgccattga actggctgcc gatcacggta ttggtctggt
     4201 ggcacttcgt aatgccaacc actggatgcg cggcggcagc tacggctggc aggcggcgga
     4261 aaaaggctat attggcattt gctggaccaa ctccatcgcc gtaatgccgc cgtggggcgc
     4321 aaaagagtgt cgcattggca ctaacccgct gatcgtcgcc attccttcca cgccgatcac
     4381 catggtcgat atgtcgatgt cgatgttctc ttacggcatg ttagaagtta accgcctggc
     4441 gggccgtcag ctcccggtcg atggtggctt tgatgatgag ggcaatttga ccaaagaacc
     4501 tggcgttatc gagaagaatc gccgcatttt gccgatgggc tactggaaag gttctggcat
     4561 gtcgattgtg ctggatatga tcgctactct cctttccgac ggcgcatcgg ttgccgaagt
     4621 cacccaggac aacagcgacg aatacggcat ttcacaaatt tttattgcca ttgaagtgga
     4681 caagcttatc gacggtccca cccgcgatgc caagctgcaa cgcatcatgg attacgttac
     4741 taccgctgaa cgcgctgacg aaaaccaggc catccgctta cccggccacg aatttactac
     4801 cctgctggcc gaaaaccgcc gtaacggcat cactgttgat gacagcgtgt gggccaaaat
     4861 ccaggcgtta tgaggagata agtcatgatt tttggacata tcgcgcagcc caatccgtgc
     4921 cgtttgcccg ccgccattga aaaggcgctc gattttctgc gcgctaccaa cttcaacgtc
     4981 ctggagccgg gcgttgtcga aatcgacggc aagaatattt atgcgcaaat tatcgattta
     5041 accacacgcg aagcggtgga aaaccgtccg gaagtgcatc gtcggtatat cgatattcag
     5101 tttctggcgt ggggtcaaga gaaaatcggc attgctattg atacaggaaa taataaagtc
     5161 agcgaatcac tattagagca gcgcgatatt attttttatc atgtcagtga acatgaatca
     5221 tttatagaaa tgataccggg cagctacgct attttcttcc cgcaggatgt tcaccgacca
     5281 ggttgtattc tgcaaacagg cctctgagat acgaaaaatt gtggttaaag tcgcattaac
     5341 agcgctgaat taatcaaata atcgtctacg ccagaacgac tgggtaatgt tattgctcta
     5401 actatattaa taatttaggt tgctgcacat gcaacttgaa ttatgacaga catagcctca
     5461 aggaatagct atgaaaaaaa tacttgaagt aatactggcg attaatctcg ccgtactttc
     5521 ctgtattgtg tttataaaca ttattttaag atatggtttt cagacaagca ttttatctgt
     5581 agatgaattg tcacgttatt tatttgtctg gttaacattt attggcgcga ttgtggcttt
     5641 tatggataac gcccacgttc aggtcacctt tctggtggag aaactctccc ccgcatggca
     5701 acgacgagtc gctctggtta cccattcctt aatcttattt atttgtggcg cactggcctg
     5761 gggcgcaaca ctaaaaacaa ttcaggactg gagcgattat tcaccgatcc tcggtttgcc
     5821 catcggcctg atgtatgccg cttgtctgcc caccagcctc gttatcgcat tctttgaact
     5881 gcgtcattta tatcaactca tcacgcgtag caattctcta acgtcgccac cgcaaggagc
     5941 ttaatcatgg ctgtgctgat ttttctgggc tgtctgttgg gtggtattgc tatcggtttg
     6001 cctattgcct gggcactgtt gttgtgcggg gcggcgttaa tgttctggct ggacatgttt
     6061 gatgtccaga tcatggcaca aacgctggtg aacggagccg atagcttctc cctgctggcg
     6121 attccgttct ttgtgctggc gggtgaaatc atgaatgcgg gcggcttgtc aaaacgcatt
     6181 gttgacctgc cgatgaaact ggtggggcat aaaccaggcg ggctgggcta cgtcggcgtg
     6241 ctggcagcta tgattatggc gagcctttct ggttctgccg ttgcggatac cgccgccgtt
     6301 gccgctctac tggtgccgat gatgcgcagc gccaactacc cggtcaaccg ggcggcgggg
     6361 ctgattgcct ctggcggcat tatcgcgcca attattcccc cttccattcc gtttattatc
     6421 ttcggcgttt ccagcggatt atccatcagt aagctgttta tggcgggcat tgcccctgac
     6481 atgatgatgg gcgcaacgct gatgcttacc tggtggtggc aggccagtcg ccttaattta
     6541 ccccgccagc aaaaagcaac gatgcaggaa atctggcact cttttgtctc cggtatctgg
     6601 gctctgtttc ttccagtcat cattattggc gggtttcgct ccggtctgtt tacccctacc
     6661 gaagccgggg ccgttgctgc tttctatgcg ctgtttgtcg ccacagttat ttaccgtgaa
     6721 atgaccttcg ccacactttg gcatgtactc attggcgcgg cgaaaaccac ctcagtggtt
     6781 atgtttctgg tggcctcggc acaagtttcc acctggctga ttaccattgc tgaactgccg
     6841 atgatggttt ctgatttact gcaaccgttg gtcgattcac cgcgtctgct gtttatcgtc
     6901 attatggtgg ctatcctgat tgtcggcatg
//
LOCUS       DAJTSL010000190         6922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTSL010000190 DAJTSL010000000
VERSION     DAJTSL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.191"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..298
                     /locus_tag="OO433_003663"
     CDS             <1..298
                     /locus_tag="OO433_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6051986.1"
                     /translation="LPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVT
                     FANVFTIIPPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(449..3772)
                     /gene="mscM"
                     /locus_tag="OO433_003664"
     CDS             complement(449..3772)
                     /gene="mscM"
                     /locus_tag="OO433_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6051987.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERTREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(3794..4762)
                     /gene="psd"
                     /locus_tag="OO433_003665"
     CDS             complement(3794..4762)
                     /gene="psd"
                     /locus_tag="OO433_003665"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6051988.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(4859..5911)
                     /gene="rsgA"
                     /locus_tag="OO433_003666"
     CDS             complement(4859..5911)
                     /gene="rsgA"
                     /locus_tag="OO433_003666"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6051989.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6006..6551
                     /gene="orn"
                     /locus_tag="OO433_003667"
     CDS             6006..6551
                     /gene="orn"
                     /locus_tag="OO433_003667"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6051990.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDSFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            6762..6837
                     /locus_tag="OO433_003668"
     tRNA            6762..6837
                     /locus_tag="OO433_003668"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6795..6797,aa:Gly,seq:gcc)
BASE COUNT         1602 a         1859 c         1864 g         1597 t
ORIGIN      
        1 gcttccttac ctgttcctcg cgctggcttt cccattcttt aaagcacgtc aggatctcga
       61 cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg tggttgtcgt
      121 actggtggtg acatttgcga acgtcttcac cattattccg cctgtggttg aagccggaga
      181 ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt tagcgatggc
      241 gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc tcgactgatc
      301 atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag taatgcgtat
      361 ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct aaatgagcca
      421 cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga cctttgcccg
      481 cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc atctggaagg
      541 gcgggaatgg catatcgata ccgtgggcat ggaagccagc cagaatcagc tggtggatct
      601 catggcgtag cggcatacgg tgacccatct cagcggcata aatacgcagc tcgaaaatct
      661 gaatcccttg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg atcaccagcg
      721 agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg gcatcagcag
      781 gggccggaat cgtcaacact acgcgcgtaa ccgagtcaga gagcgaccag ttgataaact
      841 gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag tcgctgatgg
      901 tggtggcgcg ggtgttaatt ttcgtcacgc taccggtgag atcgcgaatt gtcaccgtat
      961 cgccaatgcg aatcggtttt tcgaacagga taatcagacc agagataaag ttggcgaaaa
     1021 tttcctgcaa accaaaaccg agaccaacac cgagcgcggc gaccagccac tgcaatttcg
     1081 accattcaat accaatcatc gagaagccga ccagcccgcc aatcagcatc agcagatatt
     1141 tggtgatggt ggtgatggcg tagcctgtac ccggcgttaa atccaggtgc tgcaaaatcg
     1201 ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata aacaccagaa
     1261 tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgcct tgtaccgtgg
     1321 aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg atttctgacc
     1381 acagcacaat gacagaaagc agggcgatca acatcaaaat tgagcgcacc agccgcaagg
     1441 attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt gctccttccg
     1501 ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac atctctgccc
     1561 ggcgatgctt cgcccggtca aacgccagcc tgcggcgctg gatcagcatc cagcggcgga
     1621 taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca agcctcgcta
     1681 acagcgcctg tgccgttgcc agataaccca cagccgacgc cagaatggca accaacggcg
     1741 cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg ccctctttgt
     1801 tgagatacag tgggatcccg gcctttttca ggctgagggt gaccaccgcc agcgcaccgc
     1861 aaatgaggat aaagcaaagc cgtcccagcg aaccggagaa ttcacggtcg tcgaggttat
     1921 cgaacatcat cagcgccata atcagcggca caataagccc gatgctcatc aggtagtagc
     1981 gcatcccacg ggaaacacgt tcgcgcggcc agccaaaatg agcgataaac aagccgttcg
     2041 ggcgggcaaa ggtggcgcaa atcatcacta cccacagcag cggcacggta gccgttacac
     2101 catcgccaat cgcgaccgcc agcggataag gccacgcctc gcgcaagccg taacccagcg
     2161 tcatccacag caccggtaac ggtgacgcga cgagaatcga ccagaaaaga gtgcgcaacg
     2221 tcagccagaa gtgatcctga gtcactttgc caactttcgc cgccgaacgt tcaagaaaac
     2281 gggtgaaata gcggcgcgag taaatactgc aaccgaccag aatcaacgcg ccaaacagcg
     2341 gcaaaattgt ctctttgctg gtcagcatca tcacactggc tttgcccaac tgactgaagg
     2401 tgtccagcga aatgagacga cgcagatcct gggcgatttc cagcggccag gcgatggtca
     2461 tcgggcgcac gtcagaggtc cagaacagat agcggtgcgt tgcttcgttc acctctttca
     2521 gcgcatcctc cagttgcccg ttggagactt tcagcttggt cagttccaat agtagcgtgt
     2581 cgccaccctg caacaatgag ttcagcaact cacgctgagt gcgcagttgt gcttccagaa
     2641 tacggttttg ctcggcggtc agcggctgac cgtcggcctg atggatttgc cgtagcagcg
     2701 gctgtttatt aagcagatcc tcataacgta accgttgcac acgcaactgc gccatttcgg
     2761 tatcaagctg ttgtggtttt ggcatttctg gcagccgtgc cacctgcgcc cgcagcgctt
     2821 cgccgagcag attggacgat cccagccatt gcgactgttc acgcagcgta ttcaacgcct
     2881 gccggacctg taacgtctgg ctggcagcct gacgctgttg cgaggcaacg agatccatcc
     2941 gctgcgcctg ttgattcaaa gccgccgata gttcgcggtt aattttgaat tgcgcgacga
     3001 tatctttcgg caaatcggcg ctgttttctg ccagcaattc ggtactttcc agcgcccgct
     3061 ccgcctcaag ctgacgttgg ctgtttaatt gattacgcaa ggcctgcaaa tacgcatcca
     3121 gttgctggct ctctttttcc gccagctctg agcgtaagcg cgctaattcc tggcggttat
     3181 tggcagacag ctgcgccagc tccagttcat caacgagcgc cttaagacgt gcagagtcag
     3241 actgcaacgc gaaattttgt gcctgattga gcggagtatt gccggtaagc gttcccaggc
     3301 ggcgctcgat ctcatttaac tgacggcggg cgtcggtttg ctgttgcggc agttgattca
     3361 gcgaatcggc aatctcgcgg gtgcgctcct gctcttgctg ggcctgacgg cttttatcca
     3421 gcaactggct gctgacctgg agaatttcct gattcagcgc gtcggtagac attcccggcg
     3481 acacgctgcg cggctcgtca cgcatgttgt ttaattgtgc gcgcagagta gcggagagtt
     3541 tcggataatt atcgataact tgctgatatt gtttgatgcg ctcaagggaa ccttttcgtt
     3601 cctcaagcgc atttaaggca gactggagcg cctctacgac ttccggctgt gcgggtttcg
     3661 ccgcttttgc ctgctccagt tcctgagtga tttgtttgct atcgggggcc gtcgcggcgt
     3721 acgcccccca actgaggcac caggccatca gaaaagtgat aatcaggcgc acgtcagcgt
     3781 ttcctttgat ggattagacc tggtcttttt tatcgtcaac caatgggctg gcgtcgtgtt
     3841 ctgcttcgat ctcttcagca ggaagtgggg cgggttcagc gtctggcgta acaaaggttt
     3901 cggtagatac tgccagcggc tggccaattt tcgtgacaga caggctttcc agttgctcaa
     3961 ccagattcac tttacccggt gcaaacaggt tgataacggt ggaaccgagt ttaaagcgac
     4021 ccatttcctg gcctttcagc agtgccacag aaccgtcgtt ttccccggca ggccaggtcc
     4081 agcgcttgat gataccttcg cgcggcggcg taatggtgcc cgcccagacc gtctcaatgc
     4141 tgccaacaat cgtcgctccg accagaatct gcgccattgg gccaaattcg gtatcgaaaa
     4201 ggcaaatcac gcgttcgtta cgggcaaaca gattcggcac gttctgagcg gtgagatggt
     4261 taacggagaa gagatcgccc ggcacgtaga tcatctcacg cagaataccg ttgcacggca
     4321 tgtgtacgcg atggtagtca cgcggggaga ggtaagtggt cacaaacgta ccgttgcgga
     4381 acaggtccgc catcagatag ttgcctgcca gcagggcttc gaggctgtag ttgtggcctt
     4441 tggcttgcag gattttatct tcttcgattt tacccaactg gctgataacg ccatcggcag
     4501 gcatgaccag tacattcgga tcggtatcga ttgggcgtac ttcgtcacgc agcggacgga
     4561 caaagaattc gttaaaggtg cggtagctgg cggtgtccgg cttttgcgcc tctttcatgt
     4621 cgaccttgta gtatttaacg aacagatcga taaccagttt tgtcagccat cctgcccgct
     4681 tgcttgcgcc ccaacccgcc aggcgagtaa gccatagttt cggcagaatg tactgtagcg
     4741 aaagtttaaa tgaatttaac aaggtagcct ccaggccatt gttttgtcgt tcctgatccg
     4801 gccaacatgc cggatcctga aaaaaagggg acgattctaa cgacggttag cttaattgtc
     4861 agtcatccgt atcagaaaag tttttacgcg tttttacctg cgccatgctt tccagaatac
     4921 ggtgatagtt ttcgaaacgg gtttccgcga ttttcccttc ctcaaccgct tcccggatag
     4981 cgcagcccgg atcggtatcg tgtttgcaat cgcgatattt acacagacct aaatagtcat
     5041 ggaattcgac aaagccctga gtgatttgtt ccggctccag gtgccagagg ccgaactcac
     5101 gcacaccagg ggaatcaatc acatcaccgc cgtgcgggaa gtgatacagc cgagcggcgg
     5161 tcgtggtgtg ctgaccgagc cccgagttgt cagagacatc gttggtcagg atctcttttt
     5221 gcagccccag caacgcattc agcaggctgg atttaccgac gccagactgc ccggcaaaaa
     5281 tgctgatgcg cccggtcaac gcctcttcca gcggttttag cccatcctga gtatggctgg
     5341 aaaccatcaa tacgcgataa ccgatattgc ggtagatatc catctgctcg ttgacgaacg
     5401 ccatgccttc gtcgtccagc aggtctatct tgttgagcac aataatcggc tcaatctgca
     5461 aggtttcgca ggccaccagg taacggtcga taatattgag tgacagctcc ggcaaaatgg
     5521 cggagacaat gacaatctgg tcgatgttgg cggcaatagg ttttacgccg tcgtagaaat
     5581 ccggacgcgt caacaccgag gtacgctcat gcaccgcttc cacgatccct ttgacattta
     5641 cgccttccgc cgccggttta cccggacgcc agactacacg gtcgccggtc accagcgaac
     5701 ggatggtacg gcgaatattg cagcggtgaa cgtcgccatc ggcggattcc acatcagcgt
     5761 gcataccaaa gcggctgatg acgatacctt catcaggctc gccaaacaga ttgtcgtcgt
     5821 agtcgggctt ctccttagac gttttaagac gacgctggtg attggcgttc acgcggcgct
     5881 gctggccttt ggagagttta tttttactca atcgtacaga ctcctggtcg cccctgatgg
     5941 gcaaaacatc tatgatacac gcaattgtgg atcaatatag tcactgtgaa tgggtggaaa
     6001 atagcatgag tgccaatgaa aacaacctga tttggatcga tcttgagatg actggtctgg
     6061 atcccgagcg cgatcgcatt attgagattg ccacgctggt gaccgatgcc aacctgaata
     6121 ttctggcaga agggccgacc attgcagtac atcagtccga tgaacagctg gcgctgatgg
     6181 atgactggaa cgtgcgcacc cataccgcca gcgggctggt agagcgcgtg aaagcgagca
     6241 cgatgggcga tcgggaagct gaactggcaa cgctcgaatt cttaaaacag tgggtgcctg
     6301 cgggaaaatc gccgatttgc ggtaacagca tcggacagga ccgtcgtttc ctgtttaaat
     6361 acatgccgga gctggaagcc tacttccact accgttatct cgatgtcagc accctgaaag
     6421 agctggcgcg ccgctggaag ccggaaattc tggatagttt taccaagcag gggacgcatc
     6481 aggcgatgga tgatatccgt gaatcggtgg cggagctggc ttactaccgc gagcatttta
     6541 tcaagctgta aaatttttcc tggtcacgta agcgccggaa tccacttgcg gcggcgtgat
     6601 tttgacgcta aattgaacgt cttgctgata atttcagcac ttgagataaa aacgcaaaaa
     6661 aaactttttt ggggggttgc agagggaaag atttctcgta taatgcgcct cccgtaacga
     6721 cgcagaaatg cgaaaattac gaaagcaaaa ttaagtagta cgcgggaata gctcagttgg
     6781 tagagcacga ccttgccaag gtcggggtcg cgagttcgag tctcgtttcc cgctccaaaa
     6841 tttgaaaagt gctgcaaagc acagaccacc caagcgggaa tagctcagtt ggtagagcac
     6901 gaccttgcca aggtcggggt cg
//
LOCUS       DAJTSL010000191         6791 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTSL010000191 DAJTSL010000000
VERSION     DAJTSL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.192"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            164..892
                     /gene="mpaA"
                     /locus_tag="OO433_003669"
     CDS             164..892
                     /gene="mpaA"
                     /locus_tag="OO433_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6051991.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPSSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(867..1832)
                     /gene="ycjG"
                     /locus_tag="OO433_003670"
                     /pseudo
     CDS             complement(867..1832)
                     /gene="ycjG"
                     /locus_tag="OO433_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
     gene            1951..2457
                     /gene="tpx"
                     /locus_tag="OO433_003671"
     CDS             1951..2457
                     /gene="tpx"
                     /locus_tag="OO433_003671"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6051992.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2501..4042)
                     /gene="tyrR"
                     /locus_tag="OO433_003672"
     CDS             complement(2501..4042)
                     /gene="tyrR"
                     /locus_tag="OO433_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6051993.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4190..5251)
                     /locus_tag="OO433_003673"
     CDS             complement(4190..5251)
                     /locus_tag="OO433_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6051994.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREICRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5248..6645)
                     /locus_tag="OO433_003674"
     CDS             complement(5248..6645)
                     /locus_tag="OO433_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6051995.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
BASE COUNT         1621 a         1933 c         1661 g         1576 t
ORIGIN      
        1 ctaagtcagc ataattctaa tatctgcaca tcaataaaaa tgcaaacaac ttccggcaaa
       61 aaaagtgata caagatcgat gacatagcgt tgaagaaaag gatttgataa tccgcaaaat
      121 ttgcacaaca ctgcctgtaa tacttcctga accagagacg atcatgaccg taacccgccc
      181 acgcgccgaa cgcggcgcat ttccgcccgg aacagaacat tacggacgtt cattattggg
      241 cgcgccgtta atctggtttc ctgcccctgc agccagccgc gaaagtggtt tgattctggc
      301 tggcactcac ggtgatgaaa actcttcggt cgtcacgctc tcctgtgctc tgcggacgtt
      361 gacaccttct ttgcgccgcc atcatgtggt gctgtgtgtg aatcctgacg gctgccagct
      421 ggggttacgg gccaatgcta atggtgtgga tttaaacaga aactttccgg cggcgaactg
      481 gaaggaaggt gaaacggttt atcgctggaa cagcgccgct gaagaacgtg atgtcgtatt
      541 actgacaggt gacaaacctg gctcagaacc tgaaacccag gcgctgtgcc agcttataca
      601 ccgcattcaa cctgcctggg tggtctcatt ccacgatcca ctggcctgta ttgaagatcc
      661 cagacacagc gaattaggcg aatggctggc ccaggcgttt gaattgcctc tggtaaccag
      721 cgtcggttat gaaacgccta gctctttcgg cagttggtgt gcagatctca acttgcattg
      781 catcaccgca gagtttccgc ccatctcctc cgacgaagcc agcgaaaaat atctctttgc
      841 gatggcaaac ttgctgcgct ggcatcctaa agatgcaatt cgcccgtcgt gaactgaagc
      901 gccggttcca catctaccgc cagccaggtc ggtccgtcaa gatcggcgaa actgacctgc
      961 ggcaccagcg gtaaagcggc gctaatggca cgagaggtac acaacatgca gcccagcatc
     1021 agactgaaac cttgtgcacg cgcttcagtc gccagcgcca gcgcttccgt cagaccaccg
     1081 gttttatcga gcttaatgtt aaccatctca tagcgccctt tcagcgcctt cagattgcta
     1141 cgtgtatgac aactctcatc agcacaaatc ggcaacggat gaataaaatt ctccagcgcc
     1201 gcatcgtcct gcgcaggaag gggttgttca agcatcgcaa cgcctaaatc cgccaatagc
     1261 tggcaacgcg ccgccaaccc ttatgcacgc caggattcat ttgcatcaac gatcagcgtc
     1321 gcatcgggca cagctgtgcg aattgccacc atccgctcac taataagatg gttatccagc
     1381 ttcactttca gtaatttcgc gcctgcctgc cagagtgttg atgcactatt ggccatctga
     1441 tcaggcgtac cgatgactac cgtctgtgca gtgataactg tctcgggaag cgttatgccg
     1501 atcaaatcag ccagcgattg ctgctgtttt cgcgcggcca gatcccacaa tgcacaatcc
     1561 agcgcattac gtgctgcgcc ggcagggaga attttttgca actcctcccg tgtcagccct
     1621 ttctctaatt gcgacacgac gctcataatt tgcgccatta ccgaggcatc actttctcca
     1681 taacgcggat acggcgtgca ttcgccggtg cctttaatac cctcttcttc cagttcaacc
     1741 accaccacgc gcgcttcact gcgacttccc cgggcaatca caaacggggt atgtaagggc
     1801 taggcttcct caaatacctt aacggttctc atctttactc cttgttgtcg cggtggtctg
     1861 cggattaagc gcaaatagcg tttgctgtgt tattgacagt tagcataaac taggtgtgac
     1921 gttaactata tgtaaacagg aaagataatc atgtcacaaa ccgttcattt ccagggcaac
     1981 ccggttacag tcgccaattc catcccgcag gcgggtagca aagcgcagac ttttactctc
     2041 gtggcaaaag atctgtctga cgtcaccctc ggtcagtttg cgggtaaacg caaagtgctg
     2101 aacattttcc cgagtattga taccggtgtt tgcgccgcat cagtacgtaa gtttaaccaa
     2161 ctggcaaccg agatcgacaa caccgttgtg ctgtgtattt ctgccgatct gccgttcgcc
     2221 cagtctcgtt tctgcggcgc agaaggtctg aacaacgtta tcaccctctc cactttccgt
     2281 aacgctgaat ttctgcaagc cttcggtgtg gcaattgctg atggcccact gaaaggtctg
     2341 gcagcgcgtg ccgttgtggt tattgacgaa aatgacaatg tgattttcag ccagctggtg
     2401 gatgaaatca ccaccgagcc ggattacgaa gcagctctgg ctgtactgaa agcataagtt
     2461 aatatgcctg atggtgttgc accatcaggc atattcgcgc ttactcttcg ttcttcttct
     2521 ggcttaagcc atattcccgc aacttattgg caatcgcggt atgtgaaacg ccgagacgtt
     2581 ttgccagttt gcgcgtgctg ggataattgc gataaagctg ggttaatacc gagcgttcaa
     2641 aacggctggt gatttcgtcc agcgaacctt ccatcgcatc ttcgcccacg gctaccgttg
     2701 cggcgtcata atccggcaac aaaatatcct gtggacgcag ctcataaccg tccagttgtg
     2761 tcagtgcgcg atatatagcg ttctttaact gccgcacatt tcccggccac gcataacgcg
     2821 taagtacagt attcaggtca gcggccagtt tcggacgcgg cacgccctgc tcgtcggcaa
     2881 agcgggcgac gaacagctca gttaacggca tgatgtcctg cggacagtca cgtagcggcg
     2941 gcagattgag cgtcaacacg ttcagacgat aatagagatc ttcacggaac atgccttttt
     3001 gcaccagttc gaccagattc ttctgcgtag cgcaaatcac ccgcacatcg acatgcacct
     3061 catggtcttc gccaacccga cggaaagtgc catcattaag aaaacgcagt aatttcgcct
     3121 gcatccgtgg tgacatttcc cctatttcat ccaacagcac cgaaccaccg ttcgcctgct
     3181 caaagaatcc tttcttccct tccggagcat gaccaaacag ttcactctcg accgcatctt
     3241 ccggtataga cgcacagttc agcgccaggt aaggtttgcc cgctctgggg cttgcctgat
     3301 ggcaggcgta ggcaaagaga tctttacctg tacctgtgtc acccgtaatc agcagcggcg
     3361 cgcttagcat cgccagtttc tgcgcctgtt cgacaacatg cttcattttc gggctgacgg
     3421 cgacaatttg actgaaggcg ctgacgtcct gggcggcgac attttgcaac tggcggccca
     3481 tacgaatcgt tgatcgcaac atcaccaccg caccggtcag gacgtgttga tcattttcat
     3541 cctgaagata aacaggcgta atctccatca ggaaattctg cccattaata acgacatgct
     3601 cgttatgcga atcttgcggt tcgctttcca gccaacgtaa aaaattaaag ccgttaatca
     3661 attgtgcggc ggtatggttg cgcaggcgat ccaatttttg cccaaaaagc tgacagctcg
     3721 ccgggttcgc catatccact ttgcttttca tatcgacaga gagcacaggt tcgggcaacg
     3781 cctccagtaa cgcgctcaac gccagatgct cacgttcgga aggcatccac gggacagtac
     3841 gcacatcggt aacacctgca atacggcgta tttcggccat cagactgctg aaactctcaa
     3901 actccagttc agcaaaattg aggtagattc gcccaatggg atcaatctca ataccgcgta
     3961 aatcaatgcc tcttagcacg agtagatcga gtaattcgcg ggtcagaccg agtcggtctt
     4021 cacaaaagac ttccagacgc atgggaacct tcacctgaaa aaagaacaat taatatgatg
     4081 atatgacact atttggatag caggaaggtt tctgtcaaca atcattgaca caaagacgga
     4141 aaaacgggca cttcggcgta aagatatccg gtcagcaccc gatattgcat tatttttcgc
     4201 tgggcgtttt gcctttttgc agcgtttctt tcacctgacc gataagctga cgacggaaat
     4261 ccccgaggcg aggtttgtca tcgtcaatcc acggcagcgg gcggcaaagc tccatagctt
     4321 taatcccgag tcgtgccgtc agaagtccag caccaattcc ctgagctgcg cgggtagaca
     4381 aacgagcagc gagatcttgc gacatccagt ccatccccac ttcgcgcacc agttcgctgg
     4441 ctccggcaaa agcgatattc agcaatacca gcttaaacag acgcaaacgg ctgtaatacc
     4501 ccagttcaat gccatacagc gtggcgatgc gattaattaa acgcagattg cgccaggcga
     4561 taaacgccat atcgaccaac gccagcgggc tgaccgcaat catcaacgtt gattccgccg
     4621 ccgaacggca gatttcgcgc cgcgcctggg catctaaaac tggctggacc aaatgcgcat
     4681 acaaactgac cacttcacgg tcgttttgcg tttcatggat tgaggcatac cagcgttgca
     4741 gcgctggatg agactgatca atacccgcct gctgcgccag tttttcgcaa aatgcgcggc
     4801 ctttgcccgt gccgtggctg tgcaacaaat cgcgcgcttc gtcgcgttca tgggcgcgct
     4861 gtcgcaagcg ccataagcgc cgccactctg ttaccacaga acctacgcca gcgccgataa
     4921 tcaatgcccc agcggcacat ccacccagcg ccacccagtc ctgagtttgc caggcattca
     4981 ttgtccactg tacaccctgc ccgacaacgc ttgcgccaaa cagagccagc ccgcccatca
     5041 ccattttgcg ccacaggctg cgtttcggac gtaacgctgc atccattacc gcttcaactt
     5101 gcccctcttc ttcaggcgct tcatcgagcg tggccggggc aaaattttgc gcctgatttt
     5161 cgtcaaaggt ctgctgcgcc ctgaattttg gattctgatc gacctccagc ggaccgtcga
     5221 aatcaatacg tggttttaac ggttcggtca tcgcaattta tctcctatta aaaattccag
     5281 cgcggcatca agacgaatat gcggcagcgg tttgtcgaca tccatcacct gcgggcgaaa
     5341 cgcttcaaac tggaaccctt gtttgtccca gaacgcctga ccgggcaaac gcgcaggaac
     5401 ttcaccggga taaacggtga gtggcgcacc atcgctgagc cggttaccgc gcaatgccgg
     5461 aattttctcg ccgttgacgt caataatgcc gctggtggtc gcctgcaccg aggccagccc
     5521 caggcaatcc atactaatcc cttcaaacgc cgcattttgc caggcatcct gaatcagttg
     5581 ttgcagtaat gaaaccatat tggcgtgctg atcgatggtc acatggtccg ctttagtggc
     5641 agcaaacaat agcttatcga taaccggcga aaacaaacgc cggaacaagg tacgctgccc
     5701 gtagtggaaa ctttgcatca gctgcgtcag tgccagacgc atatcattaa atgcctgtgg
     5761 cccactgttg agaggttgca ggcaatcaac cagcacaatc tggcggtcaa agcgcagaaa
     5821 atgattctta tagaacccct tcaccacctt ctcgcagtaa taattaaacc gctcgcgcag
     5881 cattccggca ttggtgtgct tatcggcctg cgccagtttg gactcgcccc aggtatcgac
     5941 atccggccac gggaagaatt gcagcgcggg cgcacctgcc atatctcctg gcaagacaaa
     6001 gcgccctggc tgaataaagt gcagcccctg ctgtttacag tggtggagat aatcggtcca
     6061 cgcggcggca atgtctgcca gccggttttc gtcggcaggt gctagcgggt ccagcccttc
     6121 gcacatcatt cgccatttga ccgaccattc gccgcgctga ccattgagta agcccgtcat
     6181 ctggcgtgac cagcttaaat agtcctgcgc cagcatcggc aggtcgagca accattcgcc
     6241 gggataatcc acaatctcca gatacagcgt ggaggtgtcc ttgaagtggc gtagcagcga
     6301 atcgttcgat ttaaaacgta gcgccaggcg gatttcactg acgccgcgcg ttggcgtcgg
     6361 ccaggcggga ggatcgccat ataactgcgc cagtccttca tcatatgtga agcgcggaat
     6421 gccaaagtca cgctgaggaa tgcgttttac gcccagcagg cgctcttcac gcaccgcgct
     6481 taacagcggc aagcgtgctc cggcgtgaat attgagcaac tgattgacca tagcagtgat
     6541 aaacgctgtt ttgccgctgc ggcttagtcc ggttacggcg aggcgcagat gtctgtcgac
     6601 accccgattc accagcgcat taagttcatt tttaagtcgc ttcattgctg tcctgtgctg
     6661 ctctgaagac cagaaaaaaa tcattcgtaa ggatatgaat gtgggggcca ggccattgat
     6721 tatcaactgc ccgtgttgtc atcgacgggc gaactccata agatgtgcag cattcacgtt
     6781 cggacggagt t
//
LOCUS       DAJTSL010000192         6788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTSL010000192 DAJTSL010000000
VERSION     DAJTSL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.193"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..213)
                     /locus_tag="OO433_003675"
     CDS             complement(<1..213)
                     /locus_tag="OO433_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6051996.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEGASRLSVPEGTLGQW
                     VTAARKGLNTPGSRTVAELESEVMQLR"
     gene            complement(903..2612)
                     /locus_tag="OO433_003676"
     CDS             complement(903..2612)
                     /locus_tag="OO433_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6051997.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(3044..3763)
                     /locus_tag="OO433_003677"
     CDS             complement(3044..3763)
                     /locus_tag="OO433_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6051998.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3888..4451)
                     /locus_tag="OO433_003678"
                     /pseudo
     CDS             complement(3888..4451)
                     /locus_tag="OO433_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4435..4693
                     /locus_tag="OO433_003679"
                     /pseudo
     CDS             4435..4693
                     /locus_tag="OO433_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4802..5380)
                     /locus_tag="OO433_003680"
     CDS             complement(4802..5380)
                     /locus_tag="OO433_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6051999.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTCIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKDLYKCV"
     gene            complement(5801..6038)
                     /locus_tag="OO433_003681"
                     /pseudo
     CDS             complement(5801..6038)
                     /locus_tag="OO433_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(6225..6654)
                     /locus_tag="OO433_003682"
                     /pseudo
     CDS             complement(6225..6654)
                     /locus_tag="OO433_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1847 a         1558 c         1255 g         2128 t
ORIGIN      
        1 acgcagttgc ataacttcag attccagctc agccactgtg cgggagcctg gtgtattgag
       61 cccttttctt gcggcggtaa cccattgtcc taaagtgcct tcaggaacag ataatcggga
      121 agcgccttca ctgatcgaaa gctgattttc aggaaccgtt ttgacggctt cggctttaaa
      181 ctccgtagag taacgttggg tttttctgct cattattggc tccttctgat gccattctat
      241 ttcaggaagg agtgtccgtt aaactcaggc tacctcagat aagcttgata caactggtct
      301 ggtatataca gtttctttga gtgcggttac gtccacagag gtaacggtaa aagtaacccc
      361 ttgtcgggac cactccgacc gcgcacagag gcattttcat cgctgacagg ccgcattgcc
      421 tcgcagggct ggaggaggat cggtgatatc cggacgaccg taccggctta cgccgggctt
      481 cggcggtttt actcctctgc catgtgcaac aacggagtgc cgccttacat gccgctggcg
      541 cggcatatcc tggtaacatt tttgttgata ccaccttccg gccttatcct gaccagtggg
      601 ctttcctggc atcgatgata ccgatgaacg cgcatgatat tgagccgacg atattacggg
      661 caactggaaa tactcaccct ctggatgtga cctttatcca tgaagaagat ttggccatac
      721 catggaaacc tgaacaatca tcagtttacg cccatgtaaa cccctacggg atatttgaac
      781 tcgatatgga aacaaggttg ccaatcgagg tggttgcctg aaagttggca gttacccggt
      841 cactacacct tgttggagtg aacatatcaa aaacaggtag ttacacaatt taaattacag
      901 tctcataaaa aatgattaaa tctatttatt atttcaataa acgttttttt catcttcttt
      961 gaaaattgtt ctgctatagt atccggttcc agattgttct cttttattat tctacaatac
     1021 tctaatattg tttctttgtc tctatttact acagatacaa atacaccagg aataccatct
     1081 ttattagttg cggacagcat ttcgaccaat cgtcttgtgg taaaactata gttttttaga
     1141 atattacaaa aggcacgtat tgtttcggta tggccattat aaaatgccat aaaaagtcct
     1201 gatgtaccgt tgggattttt tatccataat aattcatcta tatattcctg gtgagggggg
     1261 agtattttta gtatatctcc atacgccata atggcctttg tgtgcccatt ttgaagtgcc
     1321 ataaacaacc catgctttac tttcaataat tctttaattt gttcactact aagcattttc
     1381 ttattaagca gacttccgaa catacgaata ctatctgcat gtccattctg caatgcaaaa
     1441 aataacccag gtaaatcatg gctgtctttc gcagctaata gatataatct cttatcggaa
     1501 gttaacgggg ttttaaagac tatttcccca taggctttta ttgtgtctac atgtccgttc
     1561 ataaaagaca tgcataaacc cggcacattg tttgccccta ccgcagcaag caatgtaagt
     1621 attgtttctt gactaagatt gcacatattt aatatcttac catatacttg aataatgtct
     1681 gaacatccgt tttgaagtgc tatataaaat gcaggagtac catcagagct tctcgtggcc
     1741 agaaggttca ccagtttaga ttgagaaatt tcaccatttt tgtatttttc catcagtata
     1801 aataatactt ctgtcagatt ctttgttatc gcatgatata taactctgga attgatcaat
     1861 tcatctggta aaagtttcac tgtgtttgtt agaggggaat tatgcctatc ttcaaatatt
     1921 acctgcccgg gttgctccag accataaatt ttccaataaa aatcatcaaa aaaatcctta
     1981 gcggataatt gactcagttc gaaatggcta tcacaagtaa atacagtacg tttatgagta
     2041 acagttcgat taggatcgaa aaactgaaca acccattttt cactttcttg tacgcgcttt
     2101 attttcaacc ctaacgccat ttggtggttt aatgtgcatg ctgataatgt tctgacaaag
     2161 tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa ctgttcccca ctttttattt
     2221 tccacaaaat aaatataagc tggtgatttt gaaaaatctt ccgttttttc caaacataca
     2281 ttttttgcaa tagcatcagg ggtgctaaga agctgatagt ttaccttccc atttgtttcc
     2341 ataaaattca tttcccaata ggaagctagg tgacggcaaa ctatttgtct tccatatata
     2401 tcttttgcca ctccatttat atttaccttc acatttctct tttctgaaaa ataaggtgct
     2461 gttttattct taaactgaat tagttcactg gagtttgaga ccctcataat agaacttgtt
     2521 attgttctat tatctctgtt aacagacata tctgttgata acgaattttt tgttgaaaat
     2581 atactagatg aaaatgtttt atttaacggc atagaagaca accatagaga tgttataata
     2641 ctattagatt gcagttacta atagtaaaag cctccttaca aggaggtagc ttattcagaa
     2701 tgctcctata agatagcgag ctcgtcaacg atacagatga tctgattata ttgtgcaaaa
     2761 ggagaacagg aaaataggca ctggaaaaga tgaaggcctg atgggagcat taactaatgc
     2821 aaaatccgac aggttaatat cccgaaaagc aatcaaacga ctcaaacagg agatgcacgc
     2881 tgaagtggtg gacaactgtt cggatttcag atgagtagct cagcagttta tgagccgaat
     2941 gctctgtcac ggtcaggaat caggctgcat acgctgtgcc aaatctaatc gtagttattc
     3001 taaccagttt tccagctaca aggtggtgta gctggcatct tctctactct atcatcaaac
     3061 gataaaatgg ttcctctcgt aacatttgct cttgcctttc acttccatct cgatcgcttc
     3121 gtagttcatt acgatagctg acatatttcc agtcttcagg tctaacatct gacgccggat
     3181 actccccggg agcgattttg cttcttaata gattagactc gagacacatt ataaattgtc
     3241 gtattttatg aagtaataaa gcactatatt gcgagcattc ctgatctgat tttacatata
     3301 gcgtaaactg agctcctatc cccacacgag attgctgaga aactcgattc atatcagtta
     3361 tcttccattt atctatagga ctgtcctctg aaaataataa accagacaaa atttggaatg
     3421 ccagaggaac ttgctcccta gctatactaa tatgaaactt gtccccaaca aaatccccct
     3481 tactctgagg attttcacga catgcatgaa taaacacatc tccactatgg ttcacaatct
     3541 ggaatccttg gtacatgaca gaaaaaaatt gacatatatc agagtaagtt ggcacgttcg
     3601 atgctacttt gaaatagtac gctgggtatt gccgtatttg gttgtacaat atattgaagt
     3661 tatttttcaa tctttcattc gcagaaagca tctgcggtat ttcagacctc acaacattca
     3721 aagaatctaa atttagtttt agacagggct tttttatggg catagaaaac gtcctctata
     3781 aaatagatat attatcttat attgattgtt tatcatcttt tataaaacga tacattatat
     3841 gtaatgtatg taagcgtaag ggtcttctcc gatcatggtg ggaaggctta gaacgccata
     3901 ttcagtttcc catagtggaa catcactccc agcttaaagc gctcccggtt gcggtagccc
     3961 ttggctttta ttctcagcag cctgatcttg ctgttcagtg cttccgcatt tccgtttgat
     4021 acgccatggc gcatggcgtt caggataccg tacaaccttt tccctatcgt tttagccaca
     4081 ttcttcatca tggggacgtc actgttggcc gccagcgaca gccatctctg ccagtcgttg
     4141 cgtctttcct cgctccatgg tctgctccag atatcctttg ccagttcttt cagcgcccag
     4201 cacaggctcg tcagcttcat ctgcgcacgc agccatatca gcttttcctg cctggattcg
     4261 gtcatccatt tatcgctgtg ctgccacagg aagcgagttc cctttgcctg acgacgactt
     4321 tcaacaggaa gatgcggatg ttcattctgg cgggttttat ccaccacctc acccggttgt
     4381 ttcgccacat gaaagcgatc gaaggctatt ttctcaaccg cattgggtaa gtggatgcgc
     4441 gctgctctta gtttgctgag ccagtttagc attgccttct acatgaccga tgcctgagcc
     4501 ggtataccga actttattgt cctcaaccag tcatgttata agcaagaaat gcactcagcg
     4561 gatagagcga cataatctga acctgcggac acatcttaag cggcttaccc gcaaaaccat
     4621 atgcttctca aagtctgatg atatgcacta taagatcatc ggctggtatc tgacaatcaa
     4681 ccaccaccac taaatctgcg tcacgacctg caactgagtc tggttgaaca ggctgaggcc
     4741 ctgctgaagc aacgcgaacc tctcgactct tgttggattt taccaaatag tttttctaaa
     4801 attaaacaca tttatataag tcctttgctg aacaaaacgc agcccgccat cctttttcat
     4861 tcataaattc agcaatggca gataaaactt tttcatcaag catataaaaa ataatgcaag
     4921 tagactcaat atgcctcata caaataaggc cgcacatatc cgtaatatat ttgtcaatgc
     4981 tgctataatt tgcatggtaa tatgccatgc tattatctga tgattttaca tatgcagtat
     5041 gacatatttt tccttcacga ttaacaaaac tgacaaaacc actttcacca ggagtaatat
     5101 ataaagatga atttaacgtg cgctcactaa tattcattaa ttgttgaaat tcaatggaat
     5161 agttttcagt taatgtatct attatcttgg ctttgtcttt ctttgttatc tcattgtctt
     5221 tttcggcaag aaaaattgcc actttatgac atgcattcag ctcgttaatt ttacctgata
     5281 aaactgcttt tcttaccaca tcgttgaact catcgatagt gtgtttttta ttatatatat
     5341 catgaaaaat atagcctaaa gacttaaacg agactttcat tttatattct ccttaaatat
     5401 taacaggata tatatatcac attttcctct atattttcag aaaccagcta tcattttccc
     5461 cattactggt acgtcgcttc agatatttcc gcagcaagcg ataaccctgc gcactatgag
     5521 gatgccattc attataatgt ttgaacacct tcgcgagact actttactgc tgttaacacg
     5581 tcaggttcga acataacact gatttaatca tatttcatcg ttttgacgaa gttttctgcc
     5641 attccatttt gcaccgccgt gtgtttgggg gcaaggccga ccatctgtgt ttcattcgcc
     5701 agatataagt gatgagatgg cattctccct ccttcacggc agaagcattt tgaccgttta
     5761 ggggatgcgg acgattttca tgcctgctaa gatactttcg ttactgatgc tgtccgtttt
     5821 ccaccatttc atcatcactg attggcgttg tgtttttggt cagcactcca gctttacgtt
     5881 tctcttttaa ccggtaactc tctcctttga tattcagcgt gtagcagccg atctaggatc
     5941 gctgttgcca gcacgtgatc tccgaacatt tctccccagt ctgcgacccc tttgtttgac
     6001 gtcagtatga tgctcgcttt ttcatatcga cagttcatcg ataggaattt aaatccccaa
     6061 aagattaaaa aagcaccgaa gaatgcgcgt ttattcaaca caacctgatc atcaagtaat
     6121 cggtatagac acttaatatt gcttacattg attgattcaa tgtatctctg atggtttttt
     6181 gctccgttcc cctcaacatg gacgcaactt cccctctgcg actctcagcc gcaccaccgt
     6241 attcgagcca gcggctcatg catcataacc tgctctgcca gacggtaacc cctcttcagc
     6301 cccgtaaacc gcatctgacc tccgcacagc tcgcacttca gcgggtcaac cttcagtcac
     6361 tgctgataca tccctctcct tgtgatttgc ctcgccgttt ttctcactgt ctccgttatg
     6421 gcgtacacca catcttccag taatcgccgc ttcaccggac tcaggacccc gtatcaccat
     6481 ccggaacccc ttatccgcca catgccagga gaacctttcc ataaactcgt cgccactcat
     6541 cacaaggttt tcttctcgtt ttgtccggtg gctgttgtaa cgcaggccaa tttcatcctg
     6601 accggcataa tgttccaggc ggctcatctg caccggctgc ttctccagcc agttaagcca
     6661 tcggtttccc tgctgaaaaa tatcggcaaa acggggaagc atcagaaagg cggaataacg
     6721 ccatccggcc agtgaaccgt accgcattca gggcaataca tgccgtcggc gttgataccg
     6781 gcaggaat
//
LOCUS       DAJTSL010000193         6779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTSL010000193 DAJTSL010000000
VERSION     DAJTSL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.194"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO433_003683"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO433_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6052000.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO433_003684"
     CDS             2089..2238
                     /locus_tag="OO433_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052001.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2994..3434)
                     /locus_tag="OO433_003685"
     CDS             complement(2994..3434)
                     /locus_tag="OO433_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6052002.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3652..4041
                     /locus_tag="OO433_003686"
     CDS             3652..4041
                     /locus_tag="OO433_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052003.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4085..4489
                     /locus_tag="OO433_003687"
                     /pseudo
     CDS             4085..4489
                     /locus_tag="OO433_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4596..5432)
                     /locus_tag="OO433_003688"
     CDS             complement(4596..5432)
                     /locus_tag="OO433_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6052004.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5432..5707)
                     /locus_tag="OO433_003689"
                     /pseudo
     CDS             complement(5432..5707)
                     /locus_tag="OO433_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5782..6255)
                     /locus_tag="OO433_003690"
     CDS             complement(5782..6255)
                     /locus_tag="OO433_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR6052005.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6276..6779)
                     /locus_tag="OO433_003691"
                     /pseudo
     CDS             complement(6276..6779)
                     /locus_tag="OO433_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1582 a         1755 c         1771 g         1671 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttttacatca ttccttcagc tcatacgtca
     2641 ctttcatcct ctgtgccatt tgtacatcac ccccttcctg aacgtgctga cagcgggttt
     2701 tccggctgcg ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt
     2761 tcggatggga agaacgccgg aaacaggact taccccggct gaatggtgtg aatttccgcg
     2821 ctatgcactt gtgcgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg
     2881 ggttttcggg tggtttgttg cctgttttac cggtttcccg tcagaaacgc cctgagggcc
     2941 tctcaggcgg tgctgataac agatgttatg gtaaatcaga gtcacctgca ctgttactct
     3001 gaaccggagc gaaacagccg cgaccagaat ccccgctttt tttcctgtgc cagcgcgtca
     3061 gccagacggc taatttcggc ctgtagttgt tccctctcct catttcgcag gcggttttct
     3121 gcttcccgcg ccatcttgtc ttcaagcatt agggtaacag tctgctgcag agcgtccagt
     3181 ttctgctgaa tgacgtctag ctgagcattt tccgcctggc taacgacaga tttggagtgt
     3241 ccctccttgt gcctttttgg tgtctcagaa gtgcgcagct cgccataggc ccgaatcagt
     3301 tcactggtct cgaactcccg gcgatcattt tttcctgtgc gatagctgac aaggccttta
     3361 gccatgtcac gatataattg cgagcgcgac tttcctgtta atttcagtgt ctcttgaact
     3421 gtgtgccatg ccataaaagt gacagtgtct cctgtgtgta ccgtattgta acagcagaac
     3481 atattactgt tttaacacaa aaggtctgag gctgcccgtg aggcgtgaaa aatacacaaa
     3541 aaacctgttg acggtgtggt tttgacgggc gtagtatttc gaatactgta tataaacaca
     3601 ttgttataaa tacagttttt gctgttttta aaagtcaaag aggataattt tatgagtaca
     3661 gagttaaata gcggtactgt cggtggtttt ggtcacatgg gaccaaatga cccctacaat
     3721 gatggtcata gcggctctgg aagttcggga ggtagtttta acggtggtgg taatggtggc
     3781 catgataata gtagtggttc atcttatact gcatcctttg gtctcatcac tggccgacac
     3841 ccgataactg tcccaggtac gaagaatatt aattctgtcg atctttctgc tgtcattaat
     3901 aacattatac atcatgaagg tgcacgcaat aacatgtacc tagatactgc gaacaatgta
     3961 agagtgggag tgacgggcac tggctggcaa tgtctagcaa cggcaggcat ttcggctgag
     4021 ggtaaaagaa ctttccgcta agcgatagac tgtatgtaaa cacagtattg caaggacgcg
     4081 gaacatgcct catgtggcgg ccaggacggc cagccgggat cgggatactg gtcgttacca
     4141 gagccaccga cccgagcaaa cccttctcta tcagatcgtt gacgagtatt acccggcatt
     4201 cgctgcgctt atggcagagc agggaaagga attgccgggc tatgtgcaac gggaatttga
     4261 agaatttctc caatgcgggc ggctggagca tggctttcta cgggttcgct gcgagtcttg
     4321 ccacgccgag cacctggtcg ctttcagctg taagcgtcgc ggtttctgcc cgagctgtgg
     4381 ggcgcggcgg atggccgaaa gtgccgcctt gctggttgat gaagtactgc ctgaacaacc
     4441 catgcgtcag tgggtgttga gcttcccgtt tcagctgcgt ttcctgtttg gggtcgtttg
     4501 cgggaagggg cggaatccta cgctaaggct ttggccagcg atattctccg gtgagattga
     4561 tgtgttccca ggggatagga gaagtcgctt gatatctagt atgacgtctg tcgcacctgc
     4621 ttgatcgcgg ccgcgatagc tagatcgcgt tgctcctctt ctccatccgc gttccaagct
     4681 gcggaaaggc acccataagc gtacgcctgg tcgagcaggc gacgcggatc gacgtccagc
     4741 gcacgagaga atgcgtccgc catctgtgca atgcgtctag gatcgagaca aaggtcgtct
     4801 ctgtcagccg gatcgtagaa catattggcg gcgccaaagc ccacttcacc gaccagaccg
     4861 acgggatcta tcaccagcca gccgcgactg gagaacatga tgttttcatg atgcagatcg
     4921 ccatgtagcc cacgcagttc cgaggcattg ctcatcattt gatcggctat aatcgccgcg
     4981 tggacgtagt cagtttgaca acctgcgttt tgatcatcgc gcgcccgctg aaacaaagct
     5041 gcaaagcgat cccggatcgg gagaagggca gaaggcaggg gttcctcaga tgcggcatac
     5101 agcttcgcca ttagttccgc tgcaatttcg gtcgcctggt agtcgccgtg ctcggcaacg
     5161 atgtgagaga gcattcgctc cccggcatat tcgagcaaca tcagattgtt ctcacgaccg
     5221 agcaaccgga ctgctcccct cccattgcgc cataccagat agtcggcccc gcgcagttca
     5281 tcagcaatgt cttctatagg tttcaatccc ttgacgattg caggagtccc gtctggcaat
     5341 gaaactttcc aaacgaggct ggaaaaggtg tccgcaatga gaacaggttg cgaaacgtgc
     5401 caatgagcag gaaaaacagg cggcatgaac atcaacccca agtcagaggg tccaatcgca
     5461 gatagaaggc aaggcgttcg cggtcggggg cttcgatccc caatacattg aataggacag
     5521 cgaaggcgcg ctctgcttca tctggcgctg cccagttctc ttcggcgtta gcaatcatga
     5581 gtgccaaatc ggcatagcga tctgctgttc cgagccgccc aaggtcgatc agacccgtgc
     5641 attgaagagt tttagggtcc accatgaagt tcggcatgca gggatcacca tggcaaacaa
     5701 ccatatcgtc gctatcaatc gcggaaaaag gcgtagtgcg cgaagcgaac gaagcttttt
     5761 gccgtcgatt gcatagcttt gttaaccctt tttccaaatt tgatagcaat agttaatgtt
     5821 tgaagtaaag tgttgctcaa aaacaacttc gaaggtattt ggaatactcg ggaagaaaac
     5881 atccccctct ggctcgatgt cgatcgtcga taagtggagc gtagaggcca tgggtaatgt
     5941 ttctcggtaa atttctccgc caccagacac tataacgtga ccggtgaatt cagctagcct
     6001 gtccatggcc tcttcgattg actgaaatac aactacattg tcatcatttg atgtccaacc
     6061 tgagcgggta acgaccgcgt atttcctatt ggggagtgcg cccatagatt caaacgtctt
     6121 gcgacccacc agaagccact gattgtaggt caatgcttta aaaagtagct gctccccttt
     6181 cgcggaccag ggtatgtctg gaccgcaacc aatcacgccg tttttcgctt tcgcagctat
     6241 caatgatact ttcaaggttc tcatcctggg ttaacggtgc gctcttggtc gagccgcacc
     6301 ggtagctcta gttcgacacg agccaaaaga tcgagctgcg gcgtactctt gtcctcgtcc
     6361 ggtaagaagt cgggattgac ggcattgcgg gacaccacat caacggcgcg tccgaacatt
     6421 cgcgacagcc tgcgctcaaa cggacattga tcaaccgata ggctgtgaac agcgccaagt
     6481 tgctgcccca ttgacggcca cgctttgagc aaatccgctc cagacagatc agccgccggt
     6541 actcccggaa ttgccgttat caccaagcat gcaccctcct gttcctcctg ccagttgatg
     6601 acctcggggc aagccacacc tcgacctttg agccaaatga ggcggtcacg ctctccagcg
     6661 agctcaccgc ggcgggaagc aggtgcgatt ttcgcgaagg catgcccgtc accacgtcga
     6721 aaaacaaaat caccagattc tccgcctctg acaggcaacc agtcagaatg cgattcacc
//
LOCUS       DAJTSL010000194         6729 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTSL010000194 DAJTSL010000000
VERSION     DAJTSL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.195"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            40..246
                     /locus_tag="OO433_003692"
                     /pseudo
     CDS             40..246
                     /locus_tag="OO433_003692"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            991..2904
                     /locus_tag="OO433_003693"
                     /pseudo
     CDS             991..2904
                     /locus_tag="OO433_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3032..3754)
                     /locus_tag="OO433_003694"
     CDS             complement(3032..3754)
                     /locus_tag="OO433_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6052006.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            3901..6528
                     /gene="gyrA"
                     /locus_tag="OO433_003695"
     CDS             3901..6528
                     /gene="gyrA"
                     /locus_tag="OO433_003695"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6052007.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDAAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
BASE COUNT         1617 a         1656 c         1942 g         1514 t
ORIGIN      
        1 ggctatggag atggtccttc cactgcggcg ggtggcttta tgtatctcgg cttaagtgaa
       61 gttacctttg atattgccga cggaaaaacg ctggttatcg gcaatacaga gaatgacgga
      121 gctgttgact ctattgctgg taccgggttg atcaccaaaa caggttccgg cgatctggta
      181 cttaatgcag ataacaatga ctttactggc gagatgcaga ttgaaaacgg tgaagttacc
      241 ctgggccgca gcaactccct gatgaatgtc ggcgatacgc attgccagga cgatccgcaa
      301 gactgctacg gtctgacgat agggagtatt gataagtacc agaatcaggc agagctaaac
      361 gttggctcca cccaacaaac ctttgtgcac tcattgacgg gctttcagaa tggcacttta
      421 aatatcgatg ctggtggcaa tgttactgtt aatcaaggta gttttgctgg caccatcgaa
      481 ggtgctggtc agctcaccat tgcgcaaaac ggcagctacg tgctgtcggg ggcgcagtcg
      541 atggcgctaa ccggcgatat agtcgttgat gatggtgcgg tgctttcgct ggaaggcgac
      601 gcggcagatc tcgccgctct ccaggacgat ccgcagtcga tcgtgttaaa cggcggtgtg
      661 ctcgatctct ctgatttctc cacctggcag agcggcacat catacaatga tggccttgaa
      721 gtcagtggca gcagcggaac ggttatcggc agtcaggatg tggtagatct tgcaggtggc
      781 gacaatttgc atatccgcgg cgacgggaaa gatggcgtct acgtggtggt cgatgcgagc
      841 gacgggcagg taagtctggc aaacaataat agttatttgg gcacaacaca aatcgcctcc
      901 ggtacgctga tggtgagcga caactcgcag cttggagata cccactataa ccgccaggtt
      961 atctttaccg ataagcaaca agaaagcgtg atggagatta ctgccaatgt cgatactcgc
     1021 tctacgacga ctgagcatgg gcgtgatatt gaaatgcgcg ccgacggtga agtggcagtt
     1081 gatgcggggg tagacacgca gtggggcgca ctgatggctg acagcagcgg gcagcatcag
     1141 gatgagggta gcacatttac taaaacgggg gcgggtacgc tggagctgac cgccagcggt
     1201 acaacgcagt cggcagtacg tgtcgaagaa ggcaccctga aaggcgatgt tgcggatatc
     1261 tttccttatg cttcgtcact gtgggtcggt gatggggcga cgttcgttac tggcgcggat
     1321 caggatattc agtctatcga tgctacttcc agcggcacta tcgatatcag cgatggtacg
     1381 gttttgcgtc tgaccgggca ggatacttcc gtcgccctta atgcctcatt gtttaacggc
     1441 gatgggacgc tggtgaatgc taccgatggc gtgacgttga caggtgagct taataccaac
     1501 cttgaaactg acagcctgac ctatcttgcc gacgtgacgg ttaatggtga tctgaccaat
     1561 acgtccggtg cggttagcct gcaaaatggc gtcgctggcg atacgctaac ggtaaacggt
     1621 gattatactg gcggcggtac gctactgttc gacagcgaat taaacggcga tgactcggta
     1681 agcgatcaat tggtgatgaa cggtaatact gctggcaaca caactgtggt ggttaactcc
     1741 attacaggga ttggtgagcc gacatcgaca ggcattaaag tggttgattt cgcagctgat
     1801 cccacgcagt ttcaaaacaa tgcacagttc agtctggctg gcagtggcta cgtcaacatg
     1861 ggagcgtatg actacacgct agtggaagat aacaacgact ggtatctgcg atcgcaagaa
     1921 gtaacgccgc catcgccacc tgatccagac ccggacccaa cacccgatcc tgacccaaca
     1981 cccgatccaa tccccgctta ccagccggtg ctgaatgcca aagttggcgg ttatctcaat
     2041 aacctgcggg cggcaaatca ggcgtttatg atggagcgac gcgatcacgc tggtggcgat
     2101 ggtcagacgc tgaatttacg tgttatcggc ggacgttatc attacacggc ggcagggcaa
     2161 ctggctcagc aggaagacac ttctacggtg cagcttagcg gcggcttgtt tagcgggcgc
     2221 tggggcacgg atggcgagtg gatgcttggg gcggttggtg gctacagtga taatcagggc
     2281 gacagccgct cgaatatgac cggaactcgc gccgataacc agaaccacgg ttatgccgtt
     2341 gggctgacct caagctggta tcagcacggt aattagaagc agggggcctg gctggatagc
     2401 tggctgcaat acgcgtggtt taacaatgat gtttccgaac atgaagatgg cgtggatcgt
     2461 taccattcgt cggggattat cgcctcgctg gaagccgggt atcagtggtt accggggcgt
     2521 ggtgtggtga ttgaaccgca ggcgcaggtg atttatcagg acgtgcagca ggatgatttt
     2581 accgccgcta accatgcgcg cgtgtcacaa tcgcaggggg atgatattca gacgcggctg
     2641 ggtttacaca gcgaatggcg taccgcggtt ggtgtcacac caacattaga tctgaattat
     2701 tatcacgatc cccatgcgac ggaaattgaa gaagatggca gcactatcag tgacgatgcg
     2761 gtgaagcgac ggggtgaaat aaaagtggga gtcacgggca atatcagtca gcgagtgtcc
     2821 ctgcgcggca gcgtggcgtg gcagaaaggg agtgatgatt ttgcccagac ggcagggttt
     2881 ttgtcgatga cggtgaaatg gtaatcactg tctagtcggc acggttgccg gatgcggcgt
     2941 aaacgcctta tccggcctac ggggagcatt tgtaggcctg ataagacgcg gcaagcgtcg
     3001 catcaggcat tggctcggcc aaacaacaac ctcacttatt ctgcgtgtgc agcatatagt
     3061 tcacatccac gccggggccg agtttaaaag tattagtgag cgggttgtaa tgtagcccag
     3121 ttatatgtcg ctctttaagg cttgtctgat ctacccagcc aagcagttct gccggtttaa
     3181 taaacttctt cacatcatgc gtgcctttgg gcaccatgcg caaaatatat tccgcgccaa
     3241 ccaccgccat cagccatgac ttgccgttgc ggttaagcgt cgagaaaaag acatcgccgc
     3301 ctggtttcac cagttgcgca caggctctga ccactgactg cggatcgggg acgtgctcca
     3361 gcatctccat gcaggtcacc acatcatact gcccggcatg ttttgccgcg tgctcttcca
     3421 cggtttcctg cacataatcc acctgaatgc cgctttccag cgcgtgcagt tttgccacct
     3481 gcaatggctc aaagcccata tccagaccgg tcaccgtcgc gccttcgcgc gccatactct
     3541 cggccagaat gccgccgcca caaccgacat cgagcacctt tttgccaaat aaaccgccag
     3601 cacgctcggc aatatagccc agacgcagcg ggttaatgcg gtgcaacggt ttgaactcac
     3661 cttcaagatc ccaccagcgg gaggcgacgg cttcaaattt agcgatctct tcgtggtcta
     3721 cgttatggtt ttccggcgat ttttcggcat tcattggcac ttctactccg taattggcaa
     3781 gacaaacgag tatatcaggc attggatgtg aataaagcgt ataggtttac ctcaaactgc
     3841 gcggctgtgt tataatttgc gacctttgaa tccgggatac agtagaggga tagcggttag
     3901 atgagcgacc ttgcgagaga aattacaccg gtcaacattg aggaagagct gaagagctcc
     3961 tatctggatt atgcgatgtc ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc
     4021 ctgaagccgg tacaccgtcg cgtactttac gccatgaacg tactaggcaa tgactggaac
     4081 aaagcctata aaaaatctgc ccgtgtcgtt ggtgacgtaa tcggtaaata ccatccccat
     4141 ggtgacgcgg cggtttatga cacgatcgtc cgtatggcgc agccattctc gctgcgttac
     4201 atgctggtag acggtcaggg taacttcggt tccatcgacg gcgactctgc ggcggcaatg
     4261 cgttatacgg aaatccgtct ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa
     4321 gagacggtcg atttcgttga taactatgac ggcacggaaa aaattcccga cgtcatgcca
     4381 accaaaattc ctaacctgct ggtgaacggt tcttccggta tcgccgtagg tatggcaacc
     4441 aacatcccgc cgcacaacct gacggaagtc atcaacggtt gtctggcgta tatcgatgat
     4501 gaagacatca gcattgaagg gctgatggaa cacatcccgg ggccggactt cccgacggcg
     4561 gcaatcatta acggtcgtcg cggtattgaa gaagcttacc gtaccggtcg cggcaaggtg
     4621 tatatccgcg ctcgcgcaga agtggaagtt gacgccaaaa ccggtcgtga aaccattatc
     4681 gtccacgaaa ttccgtatca ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg
     4741 gtaaaagaaa aacgcgtgga aggcatcagc gcgctgcgtg acgagtctga caaagacggt
     4801 atgcgcatcg tgattgaagt gaaacgcgat gcggtcggtg aagttgtgct caacaacctc
     4861 tactcccaga cccagttgca ggtttctttc ggtatcaaca tggtggcatt gcaccatggt
     4921 cagccgaaga tcatgaacct gaaagacatc atcgcggcgt ttgttcgtca ccgccgtgaa
     4981 gtggtgaccc gtcgtactat tttcgaactg cgtaaagctc gcgatcgtgc tcatatcctt
     5041 gaagcattag ccgtggcgct ggcgaacatc gacccgatca tcgaactgat ccgtcatgcg
     5101 ccgacgcctg cagaagcgaa aactgcgctg gttgctaatc cgtggcagct gggcaacgtt
     5161 gccgcgatgc tcgaacgtgc tggcgacgat gctgcgcgtc cggaatggct ggagccagag
     5221 ttcggcgtgc gtgatggtct gtactacctg accgaacagc aagctcaggc gattctggat
     5281 ctgcgtttgc agaaactgac cggccttgag cacgaaaaac tgctcgacga atacaaagag
     5341 ctgctggatc agatcgcgga actgttgcgt attcttggta gcgccgatcg tttgatggaa
     5401 gtgatccgtg aagagctgga gctggttcgt gaacagttcg gtgacaaacg tcgtactgaa
     5461 atcaccgcca acagcgcaga catcaacctg gaagatctga tcactcagga agatgtggta
     5521 gtgacgctct ctcaccaggg ctacgttaag tatcagccgc tttctgaata cgaagcgcag
     5581 cgtcgtggcg ggaaaggtaa atctgccgca cgtattaaag aagaagactt tatcgaccga
     5641 ctgctggtgg cgaacactca cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat
     5701 tcgatgaaag tttatcagtt gccggaagcc actcgtggcg cgcgcggtcg tccgatcgtc
     5761 aacctgctgc cgctggagca ggacgaacgt atcactgcga tcctgccagt gaccgagttt
     5821 gaagaaggcg tgaaagtctt catggcgacc gctaacggta ccgtgaagaa aactgtcctc
     5881 accgagttca accgtctgcg taccgccggt aaagtggcga tcaaactggt tgacggcgat
     5941 gagctgatcg gcgttgacct gaccagcggc gaagacgaag taatgctgtt ctccgctgaa
     6001 ggtaaagtgg tgcgctttaa agagtcttct gtccgtgcga tgggctgcaa caccaccggt
     6061 gttcgcggta ttcgcttagg tgaaggcgat aaagtcgtct ctctgatcgt gcctcgtggc
     6121 gatggcgcaa tcctcaccgc aacacaaaac ggttacggta aacgtaccgc agtggcggaa
     6181 tacccaacca agtcgcgtgc gacgaaaggg gttatctcca tcaaggttac cgaacgtaac
     6241 ggtttagttg ttggcgcggt acaggtagat gactgcgacc agatcatgat gatcaccgat
     6301 gccggtacgc tggtacgtac tcgcgtttca gaaatcagca tcgtgggccg taacacccag
     6361 ggcgtgatcc tcatccgtac tgcggaagat gaaaacgtag tgggtctgca acgtgttgct
     6421 gaaccggttg acgaggaaga tctcgatacc atcgacggca gtgccgcgga aggggacgat
     6481 gaaatcgctc cggaagtgga cgttgacgac gagccagaag aagaataatt ttacttcttc
     6541 atgccaaaag ggagctatct cccttgtttg aattgaaaag tccaggctgc aaagtctggg
     6601 cttttgtcgt attagggcac ggtaaagttt ggctgtgccc gtaaaaaatg gctggctata
     6661 cacaaggaat gtggcaatga gtggtgaaaa aaaggcgaaa ggctggcggt tctatggtct
     6721 tgtaggttt
//
LOCUS       DAJTSL010000195         6717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTSL010000195 DAJTSL010000000
VERSION     DAJTSL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.196"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            93..2093
                     /locus_tag="OO433_003696"
     CDS             93..2093
                     /locus_tag="OO433_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCR6052008.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSGRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTAV
                     GFYRLAHVLVQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            2218..2679
                     /locus_tag="OO433_003697"
     CDS             2218..2679
                     /locus_tag="OO433_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6052009.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASISATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(2720..3190)
                     /locus_tag="OO433_003698"
     CDS             complement(2720..3190)
                     /locus_tag="OO433_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6052010.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3237..3956)
                     /gene="yehT"
                     /locus_tag="OO433_003699"
     CDS             complement(3237..3956)
                     /gene="yehT"
                     /locus_tag="OO433_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6052011.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(3953..5638)
                     /gene="btsS"
                     /locus_tag="OO433_003700"
     CDS             complement(3953..5638)
                     /gene="btsS"
                     /locus_tag="OO433_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6052012.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLQLPWRDEA"
     gene            5860..5925
                     /locus_tag="OO433_003701"
                     /pseudo
     CDS             5860..5925
                     /locus_tag="OO433_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            6153..6401
                     /locus_tag="OO433_003702"
     CDS             6153..6401
                     /locus_tag="OO433_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6052013.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(6517..>6717)
                     /locus_tag="OO433_003703"
     CDS             complement(6517..>6717)
                     /locus_tag="OO433_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6052014.1"
                     /translation="KTVTTQFYIQEFSEHIAPLQDAVDLEIATEEENSLLEAWKKYRV
                     LLNCVDTSTAPDIEWPEEPDTM"
BASE COUNT         1706 a         1722 c         1641 g         1648 t
ORIGIN      
        1 gatacggccc aggcgctggt taatgtcggc gcacaaatag ccgccatgac accgggcgag
       61 ctggcatcat ggcttgcgga gaatcttcag tcatgaattc actacgtccg gaattattag
      121 aactgacacc gcaggccctg acggcgttaa gcaatgccgg ttttgttaag cgcagtctta
      181 aggaactgga aaatggcaac gtcccggaga tcagccatga gaacggcgct ttaatcgcca
      241 ccttcagtga cggtgtccgt acccagctgg cgaacggcca ggcactgaaa gaggctcagt
      301 gcagctgcgg ggccagcggt atgtgccgtc atcgcgtgat gctggtgtta agttatcaac
      361 gactttgtgc taccgcccag cctacgggaa aagaagagga atgggatccg gcaatctggc
      421 tggaagaact ggctaccctt cccgatgcca cccgcaaacg cgcacaggcg ctggtcgcta
      481 aaggcatcac cattgagttg ttctgtgcgc cgggtgaaat tccctctgcc cgcttaccga
      541 tgagcgatgt gcgtttttat tcccgcagca gtattcgttt cgcccgttgt gattgtattg
      601 aaggcacact ttgcgaacat gtcgtactgg cagtacaggc ctttgtccag gcaaaagcgc
      661 agcaagcgga attgactcat ttaatctggc agatgcgcag cgaacacgtc acatcatctg
      721 acgatccgtt tgccagcgaa gaaggcaaaa cgtgtcgtca atatgttcaa caactaagcc
      781 aggcattatg gctgagcggc atcagccagc cgcttattca ctacgaggcc gctttcagcc
      841 gcgcgcagca agcagcggaa cgctgcagct ggcgatgggt gagtgaatcg ctacggcaac
      901 tgcgcgccag cgttgatgcc ttccacgccc gcgccagcca ctatcatgcc ggagaatgct
      961 tacgtcagct tgcggcatta aacagtcgat taaactgcgc acaagagatg gcccggcgcg
     1021 acagtgttgg tgaagttcct cctgtgccgt ggcgcacggt cgttggctct ggcattgccg
     1081 gagaagcaaa gcttgatcat ctgcggctgg tgtctttagg tatgcgttgc tggcaggata
     1141 ttgagcatta tggtttacgc atctggttta ccgatcccga caccggcagt attttgcatc
     1201 tttcgcgtag ttggccgcga agtgaacagg aaaactcacc ggcagctacg cgtcggctgt
     1261 ttagttttca ggctggcgca ctggcgggtg ggcaaattgt ttcacaagca gcaaaacgca
     1321 gtgccgatgg cgagctgctg ttagctaccc gcaaccgctt aagcagcgtt gtgccgctgt
     1381 cgccagacgc ctggcaaatg ttgagcgcgc cgttacgcca gccgggcatt gtggctttgc
     1441 gggaatattt acgccagcgt ccccccgcct gcatacggcc tcttaatcag gtggataact
     1501 tatttattct gccggtcgct gagtgtattt cactcggttg ggacagcggc cgccagacgc
     1561 tggatgcgca ggtaatcagc ggcgaaggtg aagataatct gctgacgtta tcccttccgg
     1621 catctgcctg ttctcctttt gccgttgaac gcatggcggc gcttttgcaa caaacagacg
     1681 accccgtgag tctggtttct ggctttgtca gctttgtaga ggggcaattg acactggaac
     1741 cacgggtgat gatgacaaaa acccgtgcct gggcgctgga cgcggaaact gcgcctgtgg
     1801 caccgctacc ttctgccagc gttttgcctg tgccgtctac cgctcatcag ttgctgatgc
     1861 gctgccaggc gttacttatt caactgctcc ataacggctg gcgctatcag gaacagagcg
     1921 ctattagtca ggcagagttg ctggcgaatg acctcaccgc ggtgggtttt tatcggctgg
     1981 cacatgtgtt ggtacaattt cgtaatacag aaagcgaggc acgggtagaa gcaatgaata
     2041 acggtgtttt gctttgcgaa caattattcc ccatgcttca gcaacaagga tgaaatagtg
     2101 ctttttacta agagttctac tccagttccg gactgctcac gccacggtat taggcatatc
     2161 ctatatagcc cctggtgaga gtcaccagtt tcttgattaa ataaaatgga gttttacatg
     2221 aaggctttca ataagctgtt ttccctcgtt gttgcatctg ttctggtttt ctctcttgct
     2281 ggctgcggtg acaaagaaga atcgaagaaa ttcagcgcca atctgaacgg cactgaaatt
     2341 gccattacct atgtctacaa aggtgacaag gtgcttaagc aatcttctga aaccaaaatt
     2401 caatttgcct ccattagtgc aaccaccaaa gaagatgctg ccaagacact tgagccgtta
     2461 agcgccaaat acaaaaacat cgcgggtgtt gaagaaaaat taacctatac cgatacctac
     2521 gcgcaggaaa acgtgactat cgatatggaa aaagtggatt ttaaagccct gcagggtatt
     2581 tcaggaatca acgtttctgc tgaagatgcc aaaaaaggta tcactatggc gcaaatggaa
     2641 ctggtgatga aagccgctgg ttttaaagaa gtgaaataat ctgtcggcgg ccatgtttcg
     2701 tatggccgcc atacccgtct tagcttttct tcacatgctg gcgcgctgcc agtccacgca
     2761 gaaaataacg taaaaattga tcgccgcatt cgcggaagtt tttatgatcc ggtgcgcgca
     2821 tcatcgctgt aatttccggc atcgaaacgc ggaactgctg ttcggtgagg atagccagaa
     2881 tgtcatcggt tttcagcgaa aacgcgatgc gtaatttttt cagcacgatg ttgttattaa
     2941 tgcgacgttc cggctccagt gccggagcag actcatcctt gccgcgtttt tcataaatca
     3001 ggccattgag gaatgacgac aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt
     3061 ctttacgtag ccagacggcg atctgttccg cggtggcttc gacattaccc agcgccagaa
     3121 tacgcaccag gtcattatta ttggctttca aaatgtagcg cacgctgcgc agaatatcgt
     3181 tacttagcat gaggccttca ggtgttgatg aggcaaaaag ccattttagc agtcttttac
     3241 aggccaatcg cctcttttaa gcttttcaga taacggcggc tgaccggcac ggttaagcca
     3301 ttacgcaaaa tcaactcggc ctggccgtta tcttccagac gaatctcctg taaatgcgcg
     3361 aggttaacca gatactgacg atggcagcgc agtagtggtg tacgactttc cagggtacgt
     3421 aatgtcaatt cggtaaagcc ctctttccct tcgtggctgg taacgtagac accgctcatc
     3481 cgactgctga caaatgccac atctttcatt tgcagcaaat aaatccgact atgccccgta
     3541 caagggataa atttcagcgc ctgttgattt tccggtaaca gcgaaacatc ctgcttgctg
     3601 cgctcctgac gcaatcgcgc cagcgttttc tccagtcgcg cttcatcaat tggcttcagc
     3661 agataatcaa aggcatgttc ttcaaaggct ttaatagcgt attcgtcaaa cgcagtgaga
     3721 aaaacaatat acgggcgatg ttccgggtca agcatcccca ccatttccag accactgatg
     3781 cgcggcatct ggatatcgag aaacagcaca tccgggcgca gtttatgcac cgcgccgatc
     3841 ccttccacgg cgtttgaaca ctctccaacg atttcaatat cgctctgctc ctgcaaaaat
     3901 acacgcaggt tctcccgtgc taacggttca tcatcgacaa ttaagacttt aatcatgcct
     3961 cgtccctcca tggtagttgt aacgttattc gggtgtaact atcaggctca caggcgacgc
     4021 ttattccata gtcatcgcca aaccgttcac gtaaacgctt atccaccaga ttcatcccca
     4081 gcccactggc attggttacc ggttgataca aaccggcatt gtcttcgatc tccagcatca
     4141 aatgttgccc ctcacgtcgg gcgctgattg ccactcgccc tgtatccagc agttgtgatg
     4201 tcccatgttt aatggcgttt tccactatcg gttgcagggt aaacgcgggc aattgctgct
     4261 gggataattc ttgcggaata gcaatgttga cctgcaaccg cgactggaag cgcgcctttt
     4321 caatttgcag ataagcgttc acatgttcaa tttcgtcggc gagagtaaca aactccgaag
     4381 gccgctttaa gtttttgcgg aaaaaagtgg aaagatactg caccagctgg ctggcctgtt
     4441 cgctgtcgcg gcggatcacc gctttaatgg tgttaagcgc attaaacaaa aaatgaggat
     4501 tcacctgggc gtgaagcagt ttgatttctg actgggtgag catcgctttt tgccgctcat
     4561 attgcccggc aagaatctgc gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt
     4621 ttattgaact gaataaacgg tttttggctt catacaattt gatggtgccc atcacccgct
     4681 gattttcacc acgcaacgga attaccagcg tcgaccccag tttgcattgc ggatgcaaag
     4741 agcaacgata aggtacttcg ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt
     4801 ttaaggtgta agtcgaagaa atcggtttgc cgggtaaatg gtggtcgtca ccaattccag
     4861 taaaggccag caatttctct cgatcggtaa tcgcgactgc accaatatcc agctcctgat
     4921 acagcacctg tgccactttc atgctgttca cttcgttaaa cccctggcgc aaaatgcctt
     4981 ccgtcgaggc agccactttc agcgcagtgg cagaaaaagc cgaagtgtat ttttcaaaca
     5041 tcgcgcgttt atcgagcaat atacgcataa acagcgccgc gccgacggta ttggtgacca
     5101 tcattggtgc agcaatatta ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg
     5161 ccaggatgat caacatttgc accatttcag cgacgaacgt gacggcaccg gcggtaatgg
     5221 ggttaaagac tttatcagta cgcccgcgac gaatcaggat gctgtgtacc aggccgccga
     5281 gcaatccttc aacgatggtc gagatcatgc aacttagcgc ggtcatgccc cccatcgaat
     5341 atcgatgtaa cccgccggtc aggccaacca gcccaccgac gaccggaccg ccgagtaagc
     5401 cgcccattac cgcgcctatc gcacgggtat tggcaataga atcgtcaatg tgcaacccaa
     5461 accaggtgcc catgatgcag aagatggaaa agacgatgta gcagagaaat ttatgcggca
     5521 gacgaaccgt gacctgcatt aacggtatga ataatggcgt tttactcatt aaccacgcaa
     5581 tgactaaaaa aacgcacatc tgctgaagca gcagcaacac cagattaaaa tcgtacatac
     5641 ccgcaaacca cacttccctt taaaacgcgt aacatacatt gcctgcgttt aactttcttt
     5701 gaactcttgc agaaaaatga gaattcgtga gtacgatcac tcaaaatcgc ctggcaaaaa
     5761 taaaatcacc ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt
     5821 ctaaagttaa accgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt
     5881 gaagtggcgt tgctttgtga tattaaccct gtcacgttac gcgcgtgacg caacatttta
     5941 aaaaatcgac gaagatcgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt
     6001 taccatgtcg acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat
     6061 tgatgcacca taaacaatat cagaaattga tactggttat ccatacagat aaaaataaca
     6121 cctgtacata actacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag
     6181 aaaaaatcac taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac
     6241 gcatcagtaa acgttatgac gatgtagagg tgatcgtaaa agccaccagc aacgatggcc
     6301 tttctgttac acgcaccgca gataaggatt ctgcaaaaac atttgttcag gagactctga
     6361 aagatacctg ggagtctgct gacgagtggt ttgttcacta ataaacacgt aaaatcggta
     6421 acggctggaa atcattcaat actcgcacta tcgaaagttt accagccaac cgcagcacgt
     6481 cctgcatacg tcgtgtctac ggtttttctt tttcgcttac attgtgtctg gttcttccgg
     6541 ccactcaata tcaggtgcag ttgatgtatc aacacagttc agcaacaccc gatacttctt
     6601 ccaggcttcc agcaacgagt tttcttcctc cgttgcgatc tccagatcta cagcatcctg
     6661 aagtggcgca atatgctcac tgaattcctg gatgtagaac tgtgtggtga cggtctt
//
LOCUS       DAJTSL010000196         6552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTSL010000196 DAJTSL010000000
VERSION     DAJTSL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.197"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            190..2106
                     /gene="dnaK"
                     /locus_tag="OO433_003704"
     CDS             190..2106
                     /gene="dnaK"
                     /locus_tag="OO433_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6052015.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2195..3325
                     /gene="dnaJ"
                     /locus_tag="OO433_003705"
     CDS             2195..3325
                     /gene="dnaJ"
                     /locus_tag="OO433_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6052016.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGQHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3429..3581)
                     /gene="mokC"
                     /locus_tag="OO433_003706"
     CDS             complement(3429..3581)
                     /gene="mokC"
                     /locus_tag="OO433_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6052017.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            4167..5333
                     /gene="nhaA"
                     /locus_tag="OO433_003707"
     CDS             4167..5333
                     /gene="nhaA"
                     /locus_tag="OO433_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6052018.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLCPSV"
     gene            5399..6298
                     /gene="nhaR"
                     /locus_tag="OO433_003708"
     CDS             5399..6298
                     /gene="nhaR"
                     /locus_tag="OO433_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6052019.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(6336..>6552)
                     /locus_tag="OO433_003709"
     CDS             complement(6336..>6552)
                     /locus_tag="OO433_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6052020.1"
                     /translation="ENSEDGAGQVGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPL
                     FIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT         1656 a         1553 c         1718 g         1625 t
ORIGIN      
        1 atttctgcgc aaaagcacaa aaaatttttg catctccccc ttgatgacgt gggttacgac
       61 cccatttagt agtcaaccgc agtgagtgag tctgcaaaaa aatgaaattg ggcagttgaa
      121 accagacatt tcgcccctat tacagactca caaccacatg atgaccgaat atatagtgga
      181 gacgtttaga tgggtaaaat aattggtatc gacctgggta ctaccaactc ttgtgtagcg
      241 attatggatg gcaccactcc tcgcgtgctg gagaacgccg aaggcgatcg caccacgcct
      301 tctattattg cctataccca ggatggtgaa actctggttg gtcagccggc taaacgtcag
      361 gcagtgacga acccgcaaaa caccctgttt gcgattaaac gcctgattgg ccgccgcttc
      421 caggacgaag aagtacagcg tgatgtttcc atcatgccgt tcaaaattat tgctgctgat
      481 aacggcgacg catgggtcga agttaaaggc cagaaaatgg caccgccgca gatttctgct
      541 gaagtgctga aaaaaatgaa gaaaaccgct gaagattact tgggtgaacc ggtaactgaa
      601 gctgttatca ccgtaccggc atactttaac gatgctcagc gtcaggcaac caaagacgca
      661 ggccgtatcg ctggtctgga agtaaaacgt atcatcaacg aaccgaccgc agctgcgctg
      721 gcttacggtc tggacaaagg tactggcaac cgtactatcg cggtttatga cctggggggt
      781 ggtactttcg atatttctat tatcgaaatc gacgaagttg atggcgaaaa aaccttcgaa
      841 gttctggcta ccaacggtga tacccacctg ggtggtgaag acttcgacag ccgtctgatc
      901 aactacctgg ttgaagaatt caagaaagat cagggcattg acctgcgcaa cgatccgctg
      961 gcaatgcagc gcctgaaaga agcggcagaa aaagcgaaaa tcgaactgtc ttccgctcag
     1021 cagaccgacg ttaacctgcc gtacatcact gcagatgcga ccggtccgaa acacatgaac
     1081 atcaaagtga ctcgtgcgaa actggaaagc ttggttgaag atctggtaaa ccgttccatt
     1141 gagccgctga aagttgcact gcaggacgct ggcctgtccg tatctgatat cgacgacgtt
     1201 atcctcgttg gtggtcagac tcgtatgcca atggttcaga agaaagttgc tgagttcttt
     1261 ggtaaagagc cgcgtaaaga cgttaacccg gacgaagctg tagcaatcgg tgctgctgtt
     1321 cagggtggtg ttctgactgg tgacgtgaaa gacgtactgc tgctggacgt taccccgctg
     1381 tctctgggta tcgaaaccat gggcggtgtg atgacgacgc tgatcgcgaa aaacaccact
     1441 atcccgacca agcacagcca ggtgttctct accgctgaag acaaccagtc tgcggtaacc
     1501 atccatgtgc tgcagggtga acgtaaacgt gcggctgata acaaatctct gggtcagttc
     1561 aacctggatg gtatcaaccc ggcaccgcgc ggcatgccgc agatcgaagt taccttcgat
     1621 atcgatgctg acggtatcct gcacgtttcc gcgaaagata aaaacagcgg taaagagcag
     1681 aagatcacca tcaaggcttc ttctggtctg aacgaagatg aaatccagaa aatggtacgc
     1741 gacgcagaag ctaacgccga agctgaccgt aagtttgaag agctggtaca gactcgcaac
     1801 cagggcgacc atctgctgca cagcacccgt aagcaggttg aagaagcagg cgacaaactg
     1861 ccggctgacg acaaaactgc tatcgagtct gcactgactg cactggaaac tgctctgaaa
     1921 ggtgaagaca aagccgctat cgaagcgaaa atgcaggagc tggcacaggt ttcccagaaa
     1981 ctgatggaaa tcgcccagca gcaacatgcc cagcagcaga ctgccggtgc tgatgcttct
     2041 gcaaacaacg cgaaagatga cgatgttgtc gacgctgaat ttgaagaagt caaagacaaa
     2101 aaataatcgc cctataaacg ggtaattata ctgacacggg cgaaggggaa tttcctctcc
     2161 gcccgtgcat tcatctaggg gcaatttaaa aaagatggct aagcaagatt attacgagat
     2221 tttaggcgtt tccaaaacag cggaagagcg tgaaatcaaa aaggcctaca aacgcctggc
     2281 catgaaatac cacccggacc gtaaccaggg tgacaaagag gccgaggcga aatttaaaga
     2341 gatcaaggaa gcttatgaag ttctgaccga ctcgcaaaaa cgtgcggcat acgatcagta
     2401 tggtcatgct gcgtttgagc aaggcggcat gggcggcggc ggttttggcg gcggcgcaga
     2461 cttcagcgat atttttggtg acgttttcgg cgatattttt ggcggcggac gtggtcgtca
     2521 acgtgcggcg cgcggtgctg atttacgcta taacatggag ctcaccctcg aagaagctgt
     2581 acgtggcgtg accaaagaga tccgcattcc gactctggaa gagtgtgacg tttgccacgg
     2641 tagcggtgca aaaccaggta cacagccgca gacctgtccg acctgtcatg gttctggtca
     2701 ggtgcagatg cgtcagggtt tctttgccgt gcagcagacc tgtccacact gtcagggccg
     2761 cggtacgctg atcaaagatc cgtgcaacaa atgtcatggt catggtcgtg ttgagcgcag
     2821 caaaacgctg tccgttaaaa tcccggcagg ggtggacact ggagaccgca tccgtcttgc
     2881 gggcgaaggt gaagcgggcc agcatggcgc accggcaggc gatctgtacg ttcaggttca
     2941 ggttaaacag cacccgattt tcgagcgtga aggcaacaac ctgtattgcg aagtcccgat
     3001 caacttcgct atggcggcgc tgggtggcga aatcgaagta ccgacccttg atggtcgcgt
     3061 caaactgaaa gtgcctggcg aaacccagac cggtaagctt ttccgtatgc gcggtaaagg
     3121 cgtcaagtct gtccgcggtg gcgcacaggg tgatttgctg tgccgcgttg tcgtcgaaac
     3181 accggtaggc ctgaacgaga agcagaaaca gctgctgcaa gagctgcaag aaagcttcgg
     3241 tggcccaacc ggcgagcaca acagcccgcg ctcaaagagc ttctttgatg gtgtgaagaa
     3301 gttttttgac gacctgaccc gctaacctcc ccaaaagcct gcccgtgggc aggcctgggt
     3361 aaaaataggg tgcgttgaag atatgcgagc acctgtaaag tggcggggat cactccccgc
     3421 cgttgctctt actcggattc gtaagccgtg aaaacagcaa cctccgtctg gccagttcgg
     3481 atgtgaacct cacagaggtc ttttctcgtt accagcgccg ccactacggc ggtgatacag
     3541 atgacgatca gggcgacaat catcgcctta tgctgcttca ttgctctctt ctccttgacc
     3601 ttacggtcag taagaggcac tctacatgtg ttcagcatat agggggcctc gggttgatgg
     3661 taaaatatca ctcggggctt ttctctatct gccgttcagc taatgcctga gacagacagc
     3721 ctcaagcacc cgccgctatt atatcgctct ctttaaccca ttttgtttta tcgattctaa
     3781 tcctgaagac gcctcgcatt tttgtggcgt aattttttaa tgatttaatt atttaacttt
     3841 aatttatctc tttatcgcaa ttattgacga caagctggat tatttttgaa atattgtcct
     3901 aacaaataac gccgactgac aagaaattaa ttattacttt tcctaattaa tccctcagaa
     3961 atcctcacct taagctatga ttatctgagc ttagggtcac tcgtcagcgc ttacagccgt
     4021 caaaaactca tctcaccgct gatggcgaaa attctacaaa ggctcgtaaa aaacgaatta
     4081 ttcctacact ataatctgat tttaacgatg attcgtgcgg ggtaaaatag taaaaacgat
     4141 ttattcacct gaaagagaaa taaaaagtga aacatctgca tcgattcttt agcagtgatg
     4201 cctcgggagg cattattctt atcattgccg ctatcctggc gatgatgatg gccaacagcg
     4261 gcgcaaccag tggatggtat cacgactttc tggagacgcc ggttcagctc cgggttggtt
     4321 cactcgaaat caacaaaaac atgctgttat ggataaatga cgcgctgatg gcggtatttt
     4381 tcctgttagt cggtctggaa gttaaacgtg aactgatgca aggatcgcta gccagcttac
     4441 gccaggccgc atttccagtt atcgccgcta ttggtgggat gattgtgccg gcattactct
     4501 atctggcctt taactatgcc gatccgatta cccgcgaagg gtgggcgatc ccggcggcta
     4561 ctgacattgc ctttgcactt ggagtgctgg cgctgttggg aagtcgtgtt ccgttagcac
     4621 tgaagatctt tttgatggct ctggctatta tcgacgatct tggggccatc attatcatcg
     4681 cattgttcta cactaatgac ttatcgatgg cctctcttgg cgtcgcggct gtagcaattg
     4741 cggtactcgc ggtattgaat ctgtgtggtg tacgccgcac gggcgtctat attctggttg
     4801 gcgtagtgtt gtggactgcg gtgttgaaat cgggggttca cgcaaccttg gcgggggtaa
     4861 ttgtcggctt ctttattcct ttgaaagaga agcatgggcg ttctccagcg aagcgactgg
     4921 agcatgtgtt acacccgtgg gtggcgtatc tgattttgcc gctgtttgca tttgctaatg
     4981 ctggcgtttc actgcaaggc gtcacgctgg atggcttgac ctccattctg ccattgggga
     5041 tcatcgctgg cttgctgatt ggcaaaccgc tggggattag tctgttctgc tggttggcgc
     5101 tgcgtttgaa actggcgcat ctgcctgagg gaacgactta tcagcaaatt atggcggtgg
     5161 ggatcctgtg cggtatcggt tttactatgt ctatctttat tgccagcctg gcctttggta
     5221 gcgtagatcc agaactgatt aactgggcga aactcggtat cctggtcggt tctatctctt
     5281 cggcggtaat tggatacagc tggttacgcg ttcgtttgtg tccatcagtg tgacaggacg
     5341 gtttaccggg gagccataaa cggctccctt ttcattgtta tcagggagag aaatgagcat
     5401 gtctcatatc aattacaacc acttgtatta cttctggcat gtctataaag aaggttccgt
     5461 ggttggcgca gcggaggcgc tttatttaac tccacaaacc attaccggac agatccgggc
     5521 tctggaagag cgcctgcaag gcaaattatt taagcgcaag ggacgtggtc tcgaacccag
     5581 cgagctggga gaactggtct atcgctatgc cgataaaatg ttcaccttaa gccaggaaat
     5641 gctggatatt gtgaactatc gcaaagaatc caatttattg tttgacgttg gcgtggctga
     5701 tgcactttcc aaacgcctgg tcagtagcgt gctgaatgct gcagtggtag aaggcgagcc
     5761 gattcatctt cgctgcttcg aatccaccca cgaaatgcta ctggagcaat taagccagca
     5821 taaactggat atgatcattt ccgactgtcc gatagactct acgcagcagg aaggcctgtt
     5881 ctccgtgaga attggcgaat gtggcgtgag tttctggtgt acaaatccac caccagaaaa
     5941 accgttcccg gcttgtctgg aagaacggcg acttttgatt cctgggcgac gttcaatgtt
     6001 agggcgcaaa ttgcttaact ggtttaactc ccagggatta aacgtagaaa tcctcggcga
     6061 gtttgatgat gccgcgttga tgaaagcttt tggtgcaatg cacaatgcaa tcttcgttgc
     6121 cccaacgctt tatgcatatg acttttatgc cgataaaact gtcgtagaaa ttggtcgcgt
     6181 cgagaatgtg atggaagagt accatgctat ttttgctgag cggatgattc agcacccggc
     6241 ggtacagcga atctgcaata cggattattc tgcgcttttt agtccagcgg cgcgttaatc
     6301 ggcagctccc ccaaagttaa agtggaggag atagattagt tgtacataac cacgattttg
     6361 actcggctca ttatttgccc gcttgagaca ttatttccat atgtacgcgg gcgaataaat
     6421 agaggaatct gattacttcc ttcatgggga tgctgaaaag agtagtaatt gcttttacca
     6481 tactggattt cattcgtacc atcattggag agttgaaagc ctacttgacc cgcaccgtcc
     6541 tcactgtttt ct
//
LOCUS       DAJTSL010000197         6429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTSL010000197 DAJTSL010000000
VERSION     DAJTSL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.198"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            152..460
                     /locus_tag="OO433_003710"
     CDS             152..460
                     /locus_tag="OO433_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6052021.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(519..1613)
                     /gene="yaiW"
                     /locus_tag="OO433_003711"
     CDS             complement(519..1613)
                     /gene="yaiW"
                     /locus_tag="OO433_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6052022.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(1626..2846)
                     /gene="sbmA"
                     /locus_tag="OO433_003712"
     CDS             complement(1626..2846)
                     /gene="sbmA"
                     /locus_tag="OO433_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6052023.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLVA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            3198..4355
                     /gene="ampH"
                     /locus_tag="OO433_003713"
     CDS             3198..4355
                     /gene="ampH"
                     /locus_tag="OO433_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6052024.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(4356..4979)
                     /gene="iprA"
                     /locus_tag="OO433_003714"
     CDS             complement(4356..4979)
                     /gene="iprA"
                     /locus_tag="OO433_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR6052025.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FFILEGVISLRREENVLIGITQASYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(5067..>6429)
                     /locus_tag="OO433_003715"
     CDS             complement(5067..>6429)
                     /locus_tag="OO433_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6052026.1"
                     /translation="TDKLELTSGNIADHNGNVISGVFDINSSDYVLNADLVNDRTWDT
                     SKSNYGYGIVAMNSDGHLTINGNGDVDNGTELDNSSVDNVVAATGNYKVRIDNATGAG
                     TIADYKDKELIRVNDANTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMA
                     LSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVN
                     GIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFV
                     DGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYETPYGSVSG
                     LFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSN
                     NDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVK
                     YTW"
BASE COUNT         1604 a         1661 c         1551 g         1613 t
ORIGIN      
        1 ttgtgactca caacgaagat tcacgcttcg ggaatattcc caacatcaat ttaccatgat
       61 tctgatattg acaatctaaa tgacctgtca gacttgccat aagccgctgg tatcacacca
      121 gaagagaagc agatgacttc cggaggtttt tatggctgat ttcaccctgt caaaatcgct
      181 gtttagcgga aaatatcgca atgcctcttc aacgcctggc aacattgcct atgcgttgtt
      241 tgtgctgttt tgcttttggg ctggggcgca attgctgaac ctgttagtgc atgcgcccgg
      301 cgtctatgag cgtttaatgc aggtccagga aacaggtcgc ccacgggtgg aaattggttt
      361 aggtgtcggc accattttcg ggctgatccc gtttttagta ggctgcctca tttttgcagt
      421 ggtggcgcta tggctgcact ggcgacatcg ccgccagtaa gatttacgtt gccagatacg
      481 gcctaaaacg ccttatctgg catgacattc ctgtttgatc actgtttcat acaacgcgcc
      541 cgccgttcgt ctacgcgctt cgcaaaccag gccgtagtca ggttgcgggt gattttcggg
      601 ctttccagtt gaatgccagg caacatttcg cggggtagtg atttacccgt tttcgcttcg
      661 gcaagttgat aaactttctt gtacagcgcc gtctcctcaa aagagaagct gtcacctttc
      721 tctaactgac ggcgaatttc gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt
      781 gccagttcgg ttttcccggg ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc
      841 agcttcacgc cgctggcctt actgaccgcg ttctgaaacg cggcattacg gctggcgtac
      901 cagccagcgt taaaatcagc aaagcggtat atcggtgcgc tatagctggc gggataattc
      961 agtaaatggt aagtaccaaa ccacaacccg ccgcggcgac tgaagacttc ctgacgaact
     1021 gtaccgtcca ttttccacgg ataccctttg gtatgctgtt cggcaaaagc aatgctgacc
     1081 tgcatcggcc caccggtgcg caccggatta agcgaaccaa acagcgtctg ccccattggt
     1141 accatgctga tcagatcgtc aaaaatcgcg ctcaattgct tttctgtacg caccgaatct
     1201 aaccgttcgc tataactttt gccgtttggc gatttgattt ttagcgcggt atgaaccaga
     1261 aaagcaggaa tgtgcatccg ttcagcacga cggtcaatct cttgccaggc gatcttgctt
     1321 aagcccggaa cggccggatc tgcctgataa tttgactcct gctgcgccac cgccagcacc
     1381 gagcagacgt tttccagcgt cggtgccagc ccctgacttt caaaggtggt ggcgagatct
     1441 ttcgcccagg catcgcggtc tttcacgctc gcaggcatct tctgccgcac gacgctcgct
     1501 acatctatcg ctttttcgcc ctttttcaac ggttgcggag cctgactact acaccccgct
     1561 aaaaccagaa ccgccaacaa tgacagcgaa gatagatgat tgacgcgcga cattacttct
     1621 cctttttagc tcaaggtatg ggttacttcc tgaattttgt caccatccag ctcatgttca
     1681 aagctgcgca gacgtttgta gatagacatc aactcaacca gtgtggtcca tgagttaatc
     1741 aggtactgga aagcaccgcg aacctgacca aaaacgttgg taatctgcgt catcaggccg
     1801 agcgtaatcg taccggcaac aattgacgga aacagcaaga acaaaccgaa aacgttatcg
     1861 acctgcaaat agagaatgcg ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa
     1921 tagtttttcc gtacggcgct aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca
     1981 tcgtcttcac cataaaccag ctctttacgg taggcggcct ctactcgctg gtttttaaac
     2041 tccagccccg gcagtttgat ccctaccact gccagcaatc cggtccccat cagcgaccag
     2101 acgatagcgg caatcaccag accatacgga atgtgcccga taataggcag ctccggcaca
     2161 tgcgcggaga gcgttaccag caccggcagg aaggcgacca acgtcatgat ggcgttgata
     2221 aaactgacgc ccatattctc cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc
     2281 tgtgcggcac cttcgatatg acgcagttgt tgccagttcg ccatgtaata ttcgttcatc
     2341 gccgtacgcc agcggaacac atagtgactg acaaagaagt tgttcagtac actgatcacc
     2401 accgcgatca gcgcaatccc cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg
     2461 actttatgcg gcgaacttag cgcggtttga atcagatcat agaacggtgc ataccaggcg
     2521 ttgacggcga ctccgacttc caccaaaaac caggtgacga agatgatcag tgcagtaccg
     2581 agtattgacc agtattgcca acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt
     2641 cctacgcaaa caatgtagta agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg
     2701 gcgctaatcg ggatctgccc ggaagcgccg gtgatacgcg ccacccagtc tcccccaccg
     2761 gcttgccaga agataacggc aatcaatgcc caaacaaagg ccgagagaaa aaacgctccc
     2821 ggctttggga aaaaagactt aaacatatgc actcctgcta acttcttatc gttttaacaa
     2881 ctgctgtgta ttaaccgcat gaccactaca aagcgtcagc caaatgaaac cgcccattgt
     2941 gacccatagc aaggcgctta gttcgccagc ctgaccataa gaattgtttc gacggatttc
     3001 acaaaccaaa gattgtttac ataagcggat taacacgcgc aggatcacaa atgcaggcat
     3061 tggtgagcag gaaaaagatc gtgtttacgc gccgttacgt ttttaacact attattgcac
     3121 taattcgccc tttgcaatcc atcaatgagt agtataaata cgctcagtta ccttcattca
     3181 atctatggac accaccgttg aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt
     3241 cattgacctc cgtccatgcg gcgcagccga tcactgaacc ggagtttgcc tctgatattg
     3301 tcgatcgtta tgccgatcat attttttacg gcagcggtgc cacggggatg gcgctggttg
     3361 ttatcgacgg taaccagcgc gtctttcgca gttatggcga aacgcgacct ggtaataacg
     3421 ttcgcccgca gctggattcg gtcgtgcgta ttgcttccct caccaagcta atgaccagtg
     3481 aaatgctggt gaaattgctc gaccagggga ccgtgaagtt aaacgatccg ctaagtaaat
     3541 atgctccgcc aggcgcacgc gtgccaacct acaatgggac gcccattacc ctagtgaatc
     3601 tggcaaccca taccagcgcc ctgccccgtg aacagcccgg tggcgcggca catcgtccgg
     3661 tgtttgtctg gccaacgcgc gagcaacgct ggaaatacct ttctacggcg aagctgaaag
     3721 ccgcgccagg cagtcaggcg gcgtactcta accttgcgtt cgacctgctg gcagatgcac
     3781 tggcgaatgc gtccggcaag ccttataccc agttgtttga agagcagatt acccgtccat
     3841 tggggatgaa agacaccacc tacaccccct caccggatca gtgccgccgt ttaatggttg
     3901 ctgagcgcgg tgccagtccg tgcaataaca cgctggcggc aattggcagc ggcggcgtct
     3961 attccacgcc tggcgatatg atgcgctgga tgcagcagta tttatcatct gatttttatc
     4021 agcgcagtaa ccaggcagat cgcatgcaga cgttgattta tcagcgcgcg cagtttacca
     4081 aagtgattgg catggatgtc cccggcaaag cggatgcgct tggtttaggc tgggtatata
     4141 tggccccgaa agagggtcgt ccggggatta ttcagaagac aggcggtggt ggcggtttca
     4201 ttacctatat ggcgatgatc ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt
     4261 cgccgttgac gcgctttaaa aatatgagcg atggcatcaa tgatctggtg accgagctaa
     4321 gcgggaataa accgttggtt atccctgcat cctgattaat actctgaagg caaacggttg
     4381 atagcgacca gtttgccttt attcatttcg atatagccgc ctttacgcaa agcagcgaga
     4441 acttctgcga cgacagaacg cgatatgcgt gtacgttgat ggatataatt catcacacca
     4501 atacgtgatc gcaattcttc attccagtca atcattgata ataatgttgc gcggatttgt
     4561 tcgtaggaat tatgcccaat gagctgcacg tcgcgtaatt ccagaattct attttgccag
     4621 gctaaccagt aaaaagcgtc tcgccagagt tgattttgtt caataagcgt aatggtttgt
     4681 ttggctggta gatgatatcc cgtacaattt ccttctgata ttaatttgta tggtatatcg
     4741 tttttcatta aaccatcagc cagccccata atataagaag cctgggtaat accgataagt
     4801 acgttttctt ctctacgcag agagataacg ccctctaaaa taaagaaagt atcttcgcta
     4861 ttgacatcac tggaaaatat aacttgcttt tcattattaa actcgaagcg cgtaccgtat
     4921 ctggacaaac atttatcgag cttaccaaat tcctgaagag gtttaactac agataacatt
     4981 tgcgcgtcct ttgcagtaat gcccgtcaaa tccttgacgg gcattattta gattaaatta
     5041 ccagaatttc ttcggagtga agaatattac caagtatatt taacacccac gttcgcggac
     5101 cagtcttgat ctacgtcacc accaccgagg tagttagcat cggtataggc gctgaagttc
     5161 ttggtgaagc tgaactgagt acccagacca acacgtaccg cagacccttc agtaccgtta
     5221 tcgatggaat caccgttcac atcgttatcg ttgtttgagt cgtcgtagac gtaagccagt
     5281 ttgaagtacg gagtcagagc ctgatcttcg ctgtaggtga aggtataacc tgcatctaca
     5341 cccagttcat aacgcatgct gtcgtaagac tgaccgtcaa ctttcatgtc gttgctcagc
     5401 tggtagtcat caccagactg gaacagacca gaaacgctgc cgtaaggagt ctcgtaacca
     5461 gcatcaccca gtttgaagtc gtaaccggct ttcaaaccga agccccacgc gtcagagttg
     5521 gtgctaccgt caacgtaagt accgttgctc atggttgcag acaggtcgtt gttgaagtga
     5581 gagtagctca agctaccatc aacaaagacg ttgttcgcga agtgagcaga agagtagatg
     5641 taggcagtct ggctgtcttg atccacctga ccagaacggt cattcatgtc acctttagcg
     5701 aagcctgcag ccgcaccgac gatccactta gcgttgttac cgtcaatttt ggtatcaaca
     5761 ccgaccatga tgccgttaac atcctgatca tagttgatgg tgccgttgtc gccgttgaag
     5821 ttaccaccga agtagcttac ccatgcgccg ccgttatcag ccaggccatg acgagagttg
     5881 gtcagacgag taccaacggt gtcttgttcc aggttccaga tattggtgtt cgcagacggg
     5941 atgctcagcg ccatgttagc gtagtcggtc agctccatct gttgcagaac aacggtgtta
     6001 ccgcgctgtt cagcctgata ggtgtatgca cccaggtcag ctttgttagc agcagagaag
     6061 gtcgcgttgg tgttagcgtc gtttacccga ataagttctt tatctttgta atcagcgata
     6121 gtgccagcgc cagttgcgtt gtcgatacga actttgtagt taccggttgc agcaacaacg
     6181 ttatcaacag agctgttatc cagttcagta ccgttgtcta cgtcgccgtt accattgata
     6241 gtcaggtgac catcagagtt catagcaaca ataccgtaac cgtagttaga cttggaagta
     6301 tcccacgtac ggtcgttcac cagatcagcg ttcagaacgt aatcgctgct gttgatgtcg
     6361 aacacaccag aaattacgtt gccattatgg tcagcaatgt tgccgctggt cagttccagt
     6421 ttatcagta
//
LOCUS       DAJTSL010000198         6337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTSL010000198 DAJTSL010000000
VERSION     DAJTSL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.200"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            30..152
                     /locus_tag="OO433_003716"
     CDS             30..152
                     /locus_tag="OO433_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR6052027.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDT"
     gene            168..1013
                     /locus_tag="OO433_003717"
     CDS             168..1013
                     /locus_tag="OO433_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6052028.1"
                     /translation="MRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDA
                     IILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLSN
                     AAADYFIANKIDQPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDK
                     AISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELE
                     NNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATH
                     VDGLP"
     gene            1036..2547
                     /locus_tag="OO433_003718"
     CDS             1036..2547
                     /locus_tag="OO433_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6052029.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGGRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2572..3570
                     /locus_tag="OO433_003719"
     CDS             2572..3570
                     /locus_tag="OO433_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6052030.1"
                     /translation="MSASSLSLPLGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3603..4697
                     /locus_tag="OO433_003720"
     CDS             3603..4697
                     /locus_tag="OO433_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6052031.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            4709..5581
                     /locus_tag="OO433_003721"
     CDS             4709..5581
                     /locus_tag="OO433_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6052032.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5629..6051)
                     /locus_tag="OO433_003722"
     CDS             complement(5629..6051)
                     /locus_tag="OO433_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6052033.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIILGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6148..>6337)
                     /locus_tag="OO433_003723"
     CDS             complement(6148..>6337)
                     /locus_tag="OO433_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenoxybenzoate dioxygenase"
                     /protein_id="HCR6052034.1"
                     /translation="WLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSG
                     KTFDAKLLIDENIALKSL"
BASE COUNT         1467 a         1515 c         1836 g         1519 t
ORIGIN      
        1 ttgttaataa aaaccctaca ggaggcacta tgcctaaaaa aatgagaaca acccgtaatc
       61 tattattaat ggctacgcta ttaggtagcg cactatttgc cagggctgcg gataaagaaa
      121 tgaccattgg tgcaatatac cttgatacct agggatatta cgctggagtg cgtcagggcg
      181 ttcaggatgc ggcaaaagat tcttcagtac aggtacagtt aattgaaact aacgcccagg
      241 gtgatatttc gaaagaaagt acctttgttg ataccctcgt ggcgcgtaat gtcgatgcca
      301 ttattttatc ggcagtgtct gaaaatggca gtagccgtac cgttcgtcgc gccagtgaag
      361 cgggtattcc ggtgatttgc tacaacacct gtattaatca aaagggtgtc gataaatatg
      421 tctcggcgta tctggtcggc gatccactgg aatttggtaa aaaactgagt aacgctgccg
      481 ccgattattt tattgccaat aaaattgacc agccgaaaat tgccgtcatc aattgcgaag
      541 cctttgaagt ttgtgtgcag cgacgtaaag gatttgaaga agtattaaaa tcccgcgttc
      601 ccggcgcgca aattgtcgct aatcaggaag ggactgtttt agataaagcg atttccgttg
      661 gtgaaaaact gattatctcc acgccggatc tcaacgccat tatgggggag tcgggcggtg
      721 cgacactcgg cgcggtaaaa gcggtacgta atcaaaatca ggccggaaaa attgctgttt
      781 tcggttcgga tatgacaacc gaaattgctc aggagctgga aaacaatcag gtgctgaaag
      841 cggtagtgga tatttccggt aagaaaatgg gcaatgctgt tttcgcgcaa acattgaagg
      901 ttatcaataa acaagccgac ggtgaaaaag tgattcaggt gccaatcgat ctctatacca
      961 aaacggaaga tggtaaacag tggctggcaa cgcacgttga tggtctgccc taatcgctga
     1021 ttcgcagagg tccctatgtt cacggcaaca gaggcagtcc cggtagcaaa agtggtggca
     1081 ggaaataagc gttaccccgg cgtcgttgcg ctggataacg ttaacttcac gctcaataaa
     1141 ggcgaagttc gtgcgctgtt aggtaaaaac ggcgcgggca aatcgactct cattcgaatg
     1201 cttaccggca gcgaacgccc ggatagcggt gatatctgga ttggcgagac gcgactggaa
     1261 ggtgacgaag ctacgttgac tcgccgtgcc gctgaactgg ggggtcgtgc ggtttatcag
     1321 gaattaagtc tggtggaagg gctgacggtg gcagaaaacc tctgccttgg tcaatggcct
     1381 cgccgcaacg gcatgattga ttacctgcaa atggcgcagg atgcccaacg ttgcttacag
     1441 gcgctgggcg ttgacgttag tcctgaacaa cttgtttcaa cgctaagccc ggcgcaaaag
     1501 caactggtgg aaattgcacg ggtgatgaag ggcgagccgc gcgtggtcat tcttgatgaa
     1561 cccaccagct cgcttgccag tgcagaagtt gaactggtga tcagtgcggt gaaaaagatg
     1621 tcggcactgg gcgtggcggt gatttatgtc agccaccgga tggaagaaat ccgccgcatt
     1681 gcctcctgtg ccaccgttat gcgcgatggt caggtggcgg gcgatgtgat gctcgaaaac
     1741 acctccacgc atcatattgt gtcgctaatg ctagggcgtg atcacgttga tattgcgccg
     1801 gttgcccctc aggaaattgt ggatcaggcc gtactggaag tccgtgcgtt acgccataag
     1861 cccaagctgg aggatatcag ctttacgtta cgtcgcggcg aagtgcttgg cattgctggc
     1921 ctgctggggg cagggcgcag tgaattgctg aaggcgattg ttgggctgga gacgtatgaa
     1981 cagggcgaaa ttgttatcaa cggcgagaaa atcacgcgcc ccgattacgg cgacatgctg
     2041 aaacgcggca ttggctatac gccagaaaac cgcaaagaag cggggatcat tccctggttg
     2101 ggcgttgacg aaaatacagt gctgaccaat cggcaaaaaa tcagcgccaa cggagtgctg
     2161 caatggtcca ccatccgccg cctgaccgaa gaggtgatgc agcggatgac ggtcaaggcc
     2221 gccagtagcg aaacgcccat cggcacgctt tctggtggca atcagcaaaa agtggtgatc
     2281 ggtcgttggg tctatgccgc cagccagatt ttgttgctcg acgagccaac gcgcggcgtc
     2341 gatatcgaag ccaaacagca gatttaccgt attgtccgtg agctggctgc cgaaggaaaa
     2401 agcgtggtgt ttatctccag tgaagtggag gagttgccgc tggtgtgcga ccgcatcctg
     2461 ttgttacagc acggtacgtt ctcgcaggag tttcactcgc cggtcaatgt ggatgagctg
     2521 atgtccgcca ttctgtctgt gcactgatga attttaccga ggaacctgaa gatgtctgct
     2581 tcgtcattat cattgccgct gggcaagagc gtctcgctca aacaatttgt cagtcgccat
     2641 attaatgaga tcggtttgct ggtggtaatc gccattcttt atctggtctt ctccctgaat
     2701 gcgcctggct ttatctcatt gaataaccag atgaacgtgc ttcgcgatgc tgccaccatc
     2761 gggatcgccg cctgggcaat gacgctgatt attatctccg gtgaaattga tgtcagcgtt
     2821 ggaccgatgg tggcttttgt ctcggtgtgc ctggcatttt tgctgcaatt tgaagttccg
     2881 ttggcgattg cttgtctgct ggtgttgctg ttaggcgcgc tgatgggaac gctcgccggg
     2941 gtgctgcgcg gcgtgtttaa cgtgcccagt ttcgttgcca cactggggct gtggagcgcc
     3001 ctgcgcggaa tggggctgtt tatgaccaac gcactgccag tgccgattga cgaaaacgag
     3061 gtgctggact ggctgggcgg acaatttctc ggtgtgccag tatccgcgct gatcatgatg
     3121 gtgttgtttg cgctgtttgt gttcattagc cgaaaaaccg ccttcgggcg ctcggttttt
     3181 gccgttggcg gtaatgccac ggcggcgcag ttgtgcggta ttaacgttcg tcgagtgcgc
     3241 attcttatct ttaccctttc gggattatta gcggcggtga ccggcatttt gctggcggca
     3301 cgcctcggtt ccggtaacgc aggtgccgca aacggtctgg agtttgacgt catcgccgcg
     3361 gtcgtggtcg gcggtacggc actttccggc ggtcgcggtt ccctgttcgg tacattgctt
     3421 ggtgtgctgg tgattacgct aatcggtaac ggtctggtgc tgctcggtat taactccttt
     3481 ttccagcagg tggtacgcgg cgtcatcatc gtggtggcgg tgctggcgaa tatcttgctg
     3541 acccagcgaa gcagtaaagc gaaacgctaa accttaacgc ccctggcgcg ggtgcggcag
     3601 ggatgaaaat taatcgtatt gttaatgagg gatttatgaa aacgatgctg gcagcttatt
     3661 taccaggaaa ttcaaccgtc gatctgcggg aagttgcggt gccgacgccg gggattaacc
     3721 aggtactgat caaaatgaaa tcctccggga tttgcggaag cgatgtccac tatatctatc
     3781 atcaacaccg tgccacggcg gcggcacccg ataaaccgtt ataccaggga tttatcaacg
     3841 gtcatgaacc atgcgggcag attgtggcga tggggcaagg ctgtcgccat tttaaagagg
     3901 gtgatcgcgt gctggtgtat cacatttctg gctgtggttt ttgcccgaac tgccgccgcg
     3961 gctttcctat ctcttgtact ggcaaaggaa aagcggctta cggctggcag cgtgacggcg
     4021 gtcatgccga atacttgctg gcggaagaaa aagatctgat cctcctgccg gatgcgctta
     4081 gctacgaaga tggtgcgttt atcagttgcg gcgttggtac agcgtatgaa ggaattttgc
     4141 gcggcgaagt ttccggcagt gataacgtgc tggtggtcgg tctggggcca gtcggcatga
     4201 tggcgatgat gctggcgaaa ggtcgcggtg caaaacggat catcggcgtt gatatgctgc
     4261 cggaacgtct ggcgatggca aaacagttag gggtgatgga tcacggctat ttagcaacca
     4321 ccgaaggtct gccgcagatt atcgccgaac tcacccacgg tggcgcggat gttgcgctcg
     4381 attgttctgg taatgccgca ggtcgcttgc tggcactgcg atccaccgct gattggggac
     4441 gggtggttta catcggagaa accggaaaag tggagttcga ggtcagcgcc gatctgatgc
     4501 accatcaacg gcggattatc ggctcctggg tgaccagtct gttccatatg gaaaaatgcg
     4561 cccatgatct gacggactgg aaactgtggc cgcgtaacgc cattacccat cgcttctcgc
     4621 tggaacaggc tggagatgcc tatgcgctga tggcgagcgg caaatgcggg aaagttgtga
     4681 ttaacttccc ggattaagga gttagcggat gaccatctat accttatctc acggaccgct
     4741 aaagctggat gtctctgacc agggcggcgt aatcgaaggc ttctggcgtg acacgacgcc
     4801 gcttttacgt cctggtaaaa aaagcggtgt ggcaaccgat gcttcctgtt ttccgctggt
     4861 gccgtttgca aaccgggtga gcggcaatcg ttttgtctgg caggggcgtg agtatcaact
     4921 gcaaccgaat gttgagtggg atgcgcacta tctacacggc gatggctggc tgggcgagtg
     4981 gcaatgtgtc tcgcacagcg atgatagctt gtgtctggtg tatgaacatc gcagcggtgt
     5041 ctatcactat cgggtaagtc aggcgtttca tttaacggcg gatacgctga cggtgacgct
     5101 ctctgtcacc aatcaagggg cagagacgct gccatttggt accggctggc atccttattt
     5161 tccgttgtca ccacaaacgc ggattcaggc gcaggcgagc ggttactggc tggagcggga
     5221 gcagtggctg gcgggtgagt tttgcgagca gctaccgcag gaactggatt ttaaccagct
     5281 cgcgccgttg ccgcgccagt gggtgaacaa tggttttgcc ggatggaatg gtcaggcccg
     5341 catcgagcag ccgcaggaag gttatgccat catcatggaa acgacgccac ctgcaccgtg
     5401 ttatttcatc tttgtttccg accctgcgtt tgataaagga tatgcgtttg atttcttctg
     5461 tctggaaccg atgagccatg cgccggatga tcatcatcgc ccggaagggg gtgatctcat
     5521 tgcgctagca ccaggggaat caacaacttc agagatgtcg ttgcgggtag agtggctgta
     5581 atttattaaa tgccggataa ggcgttcacg ccgcatccgg catctttcct aacgccgatc
     5641 gagagaaatt gcccccggcc cggtaattgc caatagcaag aacgcgccag cgatactgac
     5701 attcttccag aaattaatca tatttggccc aaccgcatcg ccggtcatat cccagtaatg
     5761 gtgaccaatc accgccgtac ccagcgtgta gaaaataaac agcaccgcca gcggacgggt
     5821 gaaaaagcca agcacgatta atatcgcggc gggcacttcc ataactaccg caataatcgc
     5881 tgccagcatc ggcattggcg cgcccaacga ggccatatat tggaccgtac cgtcaaagcc
     5941 catcattttg ggaaaaccca aaataatgaa aattaagacc acggcgatac gggcaattaa
     6001 taacaaaacg gggcgggcag ctccaaaatc aaaataacgt agtgtgttca taaccaatcc
     6061 ctttgcaaga taatgtgatt taaacgtaat acacactctt gcacctcgcc acgccccagg
     6121 cgttgagata ttcgctaatt atcctggtta cagtgattta agcgcgatgt tctcatctat
     6181 cagcagtttc gcatcaaacg ttttgccgct ctggatccat ttgcgaattg ggcgaatctc
     6241 acgcccctga ttcagcgtga ccgcaccgat aagcacgccg ttttgcagat taaaccaaat
     6301 cgccttctga gtttccgggt tgccacgaca aagccag
//
LOCUS       DAJTSL010000199         6280 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTSL010000199 DAJTSL010000000
VERSION     DAJTSL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.201"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            111..2408
                     /gene="xdhA"
                     /locus_tag="OO433_003724"
     CDS             111..2408
                     /gene="xdhA"
                     /locus_tag="OO433_003724"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR6052035.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAIAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGTFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            2419..3297
                     /gene="xdhB"
                     /locus_tag="OO433_003725"
     CDS             2419..3297
                     /gene="xdhB"
                     /locus_tag="OO433_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6052036.1"
                     /translation="MFDFASYHRAATLADAINLLANNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAAMSIAG
                     LQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            3294..3773
                     /gene="xdhC"
                     /locus_tag="OO433_003726"
     CDS             3294..3773
                     /gene="xdhC"
                     /locus_tag="OO433_003726"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR6052037.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(3813..5591)
                     /locus_tag="OO433_003727"
     CDS             complement(3813..5591)
                     /locus_tag="OO433_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCR6052038.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            6070..>6280
                     /locus_tag="OO433_003728"
     CDS             6070..>6280
                     /locus_tag="OO433_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR6052039.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNS"
BASE COUNT         1645 a         1589 c         1497 g         1549 t
ORIGIN      
        1 atcacacttc ctacctcccc taacgcttat cgtcgtttct ggcgtaaatc ttgcctgctt
       61 agactaaatc tttgccataa gaaccaaatg ttcaaatcga ggggatttct atggaagcgc
      121 gggaagcaac cgctacgggt gaatcatgca tgcgcgtcga tgccattgct aaggtcaccg
      181 ggcgggcacg atatactgac gattatgtta tggcgggcat gtgttatgcg aaatatgtac
      241 gtagccctat cgcacatggt tatgccgtaa gtattaatga tgaacaagcc agaagtttgc
      301 cgggcgtact ggcgattttt acctgggaag atgtgcctga tattccattc gctacagctg
      361 ggcatgcctg gacacttgac gaaaacaagc gcgataccgc cgatcgcgca ctgctaactc
      421 gccatgttcg tcatcatggt gacgccattg ccatcgtcgt ggcccgcgat gaactcacgg
      481 cagaaaaagc ggcgcaattg gtcagcattg agtggcaaga attacccgtt atcaccacgc
      541 cagaagcggc gctggcagaa gacgctgcac caatccataa cggtggcaat ttactgaaac
      601 aaagcacgat gtcgacgggt aatgtccaac aaacaatcga tgccgccgac taccaggtac
      661 aggggcacta tcagaccccc gttattcaac attgtcacat ggaaagcgta acatcgctgg
      721 cgtggatgga ggatgactcg cgaattacca tcgtttccag cacccagatc ccgcacattg
      781 ttcgccgcgt ggttggtcag gcgctggata ttccctggtc atgcgtacga gtcatcaaac
      841 catttgtcgg tggcggtttt ggtaataaac aggatgtact ggaagagcca atggcggcat
      901 tcctgaccag caagcttggc ggcattccgg tgaaagtttc ccttagccgt gaagagtgtt
      961 tcctcgcaac ccgtacccgc cacgctttta ccattgacgg gcaaatgggc gtgaaccgcg
     1021 acggaacatt gaaaggttat agtctggatg ttctgtctaa caccggcgct tatgcatctc
     1081 acgggcactc catcgcttct gcggggggga ataaagtcgc ttacctttat cctcgttgtg
     1141 cctacgctta cagttcaaag acctgctata ccaacctccc ctcggctggt gcgatgcgtg
     1201 gttatggcgc gccacaagtc gtatttgccg ttgagtctat gcttgatgac gccgcgacag
     1261 cgttaggtat tgatcctgtt gaaattcgtt tacgcaacgc cgcccgcgaa ggagatgcta
     1321 atccgctcac gggcaaacgt atttacagcg cagggttgcc ggagtgtctt gaaaaaggcc
     1381 ggaaaatctt tgaatgggaa aaacgccgtg cagaatgcca gaaccagcaa ggcaatttgc
     1441 gccgcggcgt tggcgtcgcc tgttttagct acacctctaa cacctggcct gtcggcgtag
     1501 aaatagcagg cgcgcgcctt ctgatgaatc aggatggaac catcaacgtg caaagcggcg
     1561 cgacggaaat cggtcagggt gccgacaccg tcttctcgca aatggtggca gaaaccgtgg
     1621 gggttccggt cagcgacgtt cgcgttattt caactcaaga taccgacgtt acgccgttcg
     1681 atcccggcac atttgcctca cgccagagct atgttgccgc gcctgcgctg cgcagtgcgg
     1741 cactattatt aaaagagaaa atcatcgctc acgccgcagt catgctacat cagtcagcga
     1801 tgaatctgac cctgataaaa ggccatatcg tgctggttga acgaccggaa gagccgttaa
     1861 tgtcgttaaa agatttggcg atggacgctt tctaccaccc tgaacgcggc gggcagctct
     1921 ctgctgaaag ctccatcaaa accaccacta acccaccggc gtttggctgt acctttgttg
     1981 atctgacggt cgatattgcg ctgtgcaaag tcaccatcaa ccgcatcctc aacgttcatg
     2041 attcagggca tattcttaat ccactgctgg cagaaggtca ggtacacggc ggaatgggaa
     2101 tgggcattgg ctgggcgcta tttgaagaga tgatcatcga tgctaaaagc ggcgtggtcc
     2161 gtaaccccaa tctgctggat tacaaaatgc cgaccatgcc ggatctgcca caactggaaa
     2221 gcgcgttcgt cgaaatcaat gagccgcaat ccgcatacgg acataagtca ctgggtgagc
     2281 caccaataat tcctgttgcc gctgctattc gtaacgcggt gaagatggct accggtgttg
     2341 caatcaatac actgccgctg acgccaaaac ggttatatga agagttccat ctggcaggat
     2401 tgatttgagg ataacatcat gtttgatttt gcttcttacc atcgcgcagc aacccttgcc
     2461 gatgccatca acctgctggc taacaacccg caggccaaac tgctcgccgg tggcactgac
     2521 gtactgattc agctccacca tcacaatgac cgttatcgcc atattgttga tattcataat
     2581 ctggcggagc tgcggggaat tacgctggcg gaagatggct cgctacgtat cggctctgca
     2641 acgacattta cccagctaat agaagatcct ataactcaac gtcatctccc ggcgttatgt
     2701 gctgcggcca tgtccattgc tggactgcag atccgtaacg tcgctaccta cggtggaaat
     2761 atttgcaacg gtgccaccag cgcagattct gccacgccaa cgctaattta tgacgcgaaa
     2821 ctggagatcc actccccgcg cggtgttcgt ttcgtcccga ttaatggctt tcacaccggg
     2881 ccgggcaaag tgtctcttga gcatgacgaa atcctcgtcg cctttcattt tccgccacag
     2941 ccgaaagaac acgcgggcag cgcgcatttt aaatatgcca tgcgcgacgc aatggatatt
     3001 tcaacgattg gctgcgccgc acattgccga ctggataacg gcaatttcag cgaattacgc
     3061 ctggcatttg gtgttgccgc gccaacgccg attcgctgcc aacatgccga acagactgca
     3121 caaaatgcgc cattaaacct gcaaacgctg gaagctatca gcgaatctgt cctgcaagat
     3181 gtcgccccgc gttcttcatg gcgggccagt aaagagtttc gtctgcatct catccagacg
     3241 atgaccaaaa aagtgattag cgaagccgtc gccgcggcgg ggggaaaatt gcaatgaatc
     3301 acagcgaaac aattaccatc gaatgcacca ttaacgggat gccttttcag cttcacgccg
     3361 caccaggcac gccgctctcg gaattactcc gcgaacaagg actgctaagt gtcaaacaag
     3421 ggtgctgcgt gggtgaatgt ggtgcctgta cggtgttggt cgacggcaca gcaatagaca
     3481 gttgcttata ccttgccgcc tgggctgaag gaaaagagat ccgcacgctg gaaggtgaag
     3541 cgaaaggcgg aaaactttct catgttcagc aggcttatgc gaaatccggc gcagtgcagt
     3601 gcgggttttg tacgcctggc ctgattatgg ctaccacggc aatgctggcg aaaccacgcg
     3661 agaagccatt aaccattacg gaaattcgtc gcggactggc gggaaatctt tgtcgctgca
     3721 cggggtatca gatgattgta aatacagttc tggattgcga gaaaacgaag taaaaggata
     3781 tccggcctga attcaggccg gattcactga ggttatgtgt ttaacaactc atatttctta
     3841 atcttgcgat agagcgtagc aatgccgatg cccagttcat cagcaacttg cttcttgctg
     3901 ttatgacgtg aaagcgcctc gcggatcatt tgcttttcca tctcctccag cgccgtgccg
     3961 cccgcatcat cgagtgacag gtgcgcctca ctgacctctg ttacatcact ttgctccgtt
     4021 gtgccattat tcagcagatt tggcggcaat agcgtgctgt cgataacttc acctgaagga
     4081 actacgttaa ccagatattc catcaaattg cttaactcgc gcaggtttcc gggccaacga
     4141 tgcttacgca atatttcgac gacatcggga gcaatgccag gataaaccga tcccagacga
     4201 cgggtatgca gatgtaaaaa gtaatgcacc aatagttcaa tatcttcctg acgttcacgc
     4261 agcggtggca gagttatcgg gataacatta agtcggtaga agagatcttc gcggaattta
     4321 ccttcggcaa tgaactgggc caaattctga ttagttgcag aaatgatgcg aatgtcgact
     4381 tgtattgggc tactggcacc aatcggcaga atttcacgcg cctcaatagc gcgcagtaat
     4441 ttagcctgca acattaatgg catatcacct atttcatcga gaaacagcgt gcccgtattc
     4501 gccgcctgaa tcaaccctgt tttaccgttg gcagaagcgc cagtaaatgc gcctttaaca
     4561 taaccgaaca gttcgctttc cagaagctgc tccggaatcg cggcacagtt gatagcaata
     4621 aagggtttat tccgtcttcc gctcaactta tggattgctc gggcgacgac ttctttaccc
     4681 gtgccgcttt caccaaccac cataacgctg gatgggctgg gtgcaatacg gctaatgagt
     4741 cgttttaatt gccgcataac acggcactcg ccaaccaatt gttcaatatg tggttcatca
     4801 ggtgcatttg ctacagaaaa actggtatgc gattggtgaa atgccattaa aaataattgt
     4861 cggccctgaa tgttatgcaa ttgaccaatg attaattcac ttttatcgtc ccacgaaaca
     4921 atatgctgca tatgtccatg agtaaaatta ctctcaaatg ttaatggtct gaaacggata
     4981 ggtttcccaa taatattatt ttgtacaaca ccaagtgttt ttaaggcagt ctgattaaca
     5041 aactgaaccc gactttcatc atcaacaact aatacgccct gatccatatt atcgatcatg
     5101 gtcgcgaata ttttactgat gttatctcct ggcccctgat cctccagaag tttcgaaaca
     5161 aaaatggtgg atatatggcg aacataatca gaaaattcgc gtaaattatc actgatatgc
     5221 tcttgttgct cgtgggtaac ggcaatcaaa cttatcaccc caacacaacg atcctgtaaa
     5281 atgacaggcg tacccagaaa tgctttttca cggcaatttt ctttactatc gcaaccttcg
     5341 caaaggggat cgaagcgaga ctgtgtcaca actttttcag ttttcgtttc caggacgtgg
     5401 cggagcaggc gtgagttgcc gctcaactgg cgaccaagaa acttcccata cgcgcccgtt
     5461 ccggcgacgc gacacaagtt ttcatcaacg atctcaacct caagctgcaa aacgctggca
     5521 agcattctgg caaaacgctg aattgtcggt tgaatttgca tcaatactga ctgcgtagta
     5581 gcaagctcca tagctttacc ttccagactt acttaaaagt cgatcattga agacgttgat
     5641 ggttcacaga tcatgatgat attaactcag gcgaaattgg ctttgataaa aacataagat
     5701 ttttatcatt ttctaatgaa attatggaag agatatcaca tttctatatc aatatgagaa
     5761 ttacggcggt gagtttatca aactgaagag agatagcctg cccctttatc ttatttctga
     5821 tacttagcag caaataaata acgcgataaa aaaagccaaa cgttttcgta ttttacaaac
     5881 aaccagaagc tggcatcaat ttgtgatcaa ccccacacat tatccgtcaa attagtcttt
     5941 tgcagccgcg cggataattc tggcacactt attgttagtc ccaggtatag ctgtgaaaac
     6001 accaatcact ttggcaagtc acagtgaaat aaaccacttt gcctgtcatt ccactaccgg
     6061 gactttatga tgaaaactgt taatgagctg attaaggata tcaattcgct gacctctcac
     6121 cttcacgaga aagatttttt gttaacgtgg gaacagacgc cagatgaact gaaacaagta
     6181 ctggacgttg ccgcagcatt aaaagcactg cgtgctgaaa acatctcaac caaagtcttt
     6241 aatagtggat taggtatttc cgtattccgc gacaactcca
//
LOCUS       DAJTSL010000200         6275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTSL010000200 DAJTSL010000000
VERSION     DAJTSL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.202"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO433_003729"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO433_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6052040.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO433_003730"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO433_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR6052041.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            835..1995
                     /gene="pheA"
                     /locus_tag="OO433_003731"
     CDS             835..1995
                     /gene="pheA"
                     /locus_tag="OO433_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6052042.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGLADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2038..3159)
                     /gene="tyrA"
                     /locus_tag="OO433_003732"
     CDS             complement(2038..3159)
                     /gene="tyrA"
                     /locus_tag="OO433_003732"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6052043.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLKQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3170..4240)
                     /gene="aroF"
                     /locus_tag="OO433_003733"
     CDS             complement(3170..4240)
                     /gene="aroF"
                     /locus_tag="OO433_003733"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6052044.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDLETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4448..4813
                     /locus_tag="OO433_003734"
     CDS             4448..4813
                     /locus_tag="OO433_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6052045.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFGDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4963..5481
                     /locus_tag="OO433_003735"
     CDS             4963..5481
                     /locus_tag="OO433_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6052046.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAKQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5471..>6275
                     /gene="dgcN"
                     /locus_tag="OO433_003736"
     CDS             5471..>6275
                     /gene="dgcN"
                     /locus_tag="OO433_003736"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCR6052047.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGIN"
BASE COUNT         1703 a         1602 c         1410 g         1560 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat tcccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacactgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacacac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaaccgggg ggcctttttt attgataaca aaaaaggcaa cactatgaca
      841 tcggaaaatc cattactggc gctgcgagag aaaatcagcg cgctggatga aaaattatta
      901 gcattactgg cagagcggcg cgaactggcc gtcgaggtgg gaaaagccaa actgctctcg
      961 catcgcccgg tacgagatat tgatcgtgaa cgcgatttac tggaaagatt aattacgctc
     1021 ggtaaagcgc accatctgga cgcccattac attactcgcc tgttccagct catcattgaa
     1081 gattccgtat taactcagca ggctttgctc cagcaacatc tcaataaaat taatccgcac
     1141 tcagcacgca tcgcttttct cggccccaaa ggttcttatt cccatcttgc ggcgcgccag
     1201 tatgctgccc gtcactttga gcaattcatt gaaagtggct gcgccaaatt tgccgatatt
     1261 tttaatcagg tggaaaccgg cctggccgac tatgccgtcg taccgattga aaataccagc
     1321 tccggtgcca taaacgacgt ttacgatctg ctgcaacata ccagcttgtc gattgttggc
     1381 gagatgacgt taactatcga ccattgtttg ttggtctccg gcacgactga tttatccacc
     1441 attaatacgg tctacagcca tccgcagcca ttccagcaat gcagcaaatt ccttaatcgt
     1501 tatcctcact ggaagattga atataccgaa agtacgtctg cggcaatgga aaaggttgca
     1561 caggcaaaat caccgcatgt tgctgcgttg ggaagcgaag ctggcggcac tttgtacggt
     1621 ttgcaggtac tggagcgtat tgaagcgaat cagcaacaaa acttcacccg atttgtggtg
     1681 ttggcacgta aagccattaa cgtgtctgac caggttccgg cgaaaacgac gttgttaatg
     1741 gcgaccgggc aacaagccgg tgcgctggtt gaagcgttgc tggtgctgcg caaccacaat
     1801 ctgattatga cccgtctgga atcacgcccg attcacggta atccatggga agagatgttt
     1861 tatctggata ttcaggccaa tcttgaatca gcggaaatgc aaaaagcatt gaaagagtta
     1921 ggtgaaatta cccgttcaat gaaggtattg ggctgttacc caagtgagaa cgtagtgcct
     1981 gttgatccaa cctgatgaaa aggtgccgga tgatgtgaat catccggcac tggattatta
     2041 ctggcggttg tcattcgcct gacgcaataa cacgcggctt tcactctgaa aacgctgtgc
     2101 gtaatcgccg aaccagtgct ccaccttgcg gaaactgtca ataaacgcct gcttatcgcc
     2161 ctgcttcagc aactcaatcg cctcgccgaa acgcttatag taacgtttga ttaacgccag
     2221 attacgctct gacgacataa taatgtcggc ataaagctgc ggatcctgag caaacagtcg
     2281 cccgaccatc gccagctcaa ggcggtaaat cggcgaagag agcgccagaa gttgctcaag
     2341 ctgaacattt tcttctgcca gatgcagccc ataagcaaaa gtagcaaagt ggcgcagagc
     2401 ctgaataaac gccatattct gatcgtgctc gacagcgcta atacgatgca gccgagcgcc
     2461 ccagacctga atttgctcca gaaaccattg gtatgcttcc ggcttacgtc catcacacca
     2521 gaccacaact tgctttgcca ggctaccgct gtccgggccg aacatcgggt gtaaccccag
     2581 taccgggcca tcgtgcgccg ccagcatggc ctgtaatggt ccatttttca ctgatgccag
     2641 atcaaccaga atacaatctt tcggtaaagg cggtaatttg ccgataactt gctcagtaac
     2701 gtggattggc acactaacaa tcaccattcc ggcatcggca acaatatcag ccgctcgatc
     2761 ccagtcatgt tgctccagaa tccgcacctg ataacccgag agtgtcagca tcttctcgaa
     2821 caggcgtccc atctgaccgc caccgccgac gataaccacc ggacgcagtg acggacaaag
     2881 tgttttaaat cctttgtcgt tttcactgga gtaagattca cgcatcaccc gacgcaaaac
     2941 atcctcaatc agatctggcg gtacccccag agcttccgcc tctgcacgac gcgaggccaa
     3001 catagatgcc tcgcgctccg gaacataaat aggcagtcca aagcggcttt tcacctcgcc
     3061 cacttcagca accagttcca gacgcttcgc taataaattc agcagcgctt tatcgacttc
     3121 atcaatttga tcgcgtaatg cggtcaattc agcaaccata ataaacctct taagccacgc
     3181 gagccgtcag ctgcccgttc agatcctgat gaatttcacg cagcaaggca tcggtcattt
     3241 cccagctaat gcaggcatcg gttacggata caccgtattt catttcactg cgcggttgct
     3301 cagaagactg attgccctcg tggatattac tttcgatcat cagaccaata attgagcgat
     3361 tgccatcttt gatttgagca accacggatt ctgccaccgc aggctgacgg cgataatctt
     3421 tattggaatt accgtggctg caatctacca tcagagacgg gcgcagtccc gcctgttcca
     3481 tctctttttc acattgcgca acatccgcag ggctatagtt cggcgcttta ccaccgcgca
     3541 ggatgacatg cccgtccgga ttcccctgag tttgtagcaa cgcaacctgc cctgcctggt
     3601 taatgccaac aaaacggtgc ggctgggcgg cggcgcgcat agcgttaatt gctgttgcca
     3661 gactgccgtc ggtgccgttt ttaaaaccaa ccggcatgga aagcccggag gccatttcac
     3721 ggtgagtttg cgattccgtt gtacgagcac caattgctga ccagctaaac agatcgccca
     3781 ggtattgcgg gctattcgga tctaacgctt ccgtcgccag tggcagtccc atattcacca
     3841 gctcaagcag caatttacgc gcgatctgca gcccggcttc tacatcaaaa gagccatcca
     3901 tatggggatc gttaattaac cctttccagc cgacagtggt acggggtttt tcaaaataga
     3961 cgcgcattac cagatagagg ctatcgctga cctctgcggc aagggcttta aatcgacgag
     4021 catattccag agcagtttcc agatcatgaa tggaacaagg accacaaact accagcagac
     4081 gaggatcgcg cccggcgata atatctgaaa tggttttacg cgagtcagca atctgggctt
     4141 cttgttgcag gctcaatgga aaagcggcct tcagttgttc cggagtcatt aaaacctgtt
     4201 cgtcggtaat atgtacgtta ttcagcgcgt ctttttgcat gatggcgatc ctgtttatgc
     4261 tcgtttgcga tagttgatcc tcagcgagga tgacgtaacg ataacacata aagtaaagtt
     4321 ttcaatccat atttcgtacg cttttcttta cacgaacaat ttactcgcgc tttgatgctt
     4381 atttcgtaca aataaattta cacccccttt aaaaacaatc cgctatgctt tgaaaaagga
     4441 gaaagaaatg atgaaaaagt ttatcgcccc cttattggct ttactggtta gcggatgtca
     4501 gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat
     4561 ggaagatgcg atatcgggca gcgccataaa agatgacgat gcatttggcg attcacaggc
     4621 ggatcgcggt ctatacctta aaggatatgc cgaaggacaa aagaaaactt gccagaccga
     4681 ttttacttat gcccggggac tttccggtaa aagctttcct gccagctgta acaatgttga
     4741 aagtgccagc caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat
     4801 acgattaaat taattaaaac cccacaaata aattaaacat aagattttac tcattgtgtt
     4861 ttatttctca cattgatgac gatcgcatga aaaaatgata ttttaatgag cattctgaaa
     4921 aaatatcgat gcatttcgag cgaagaaggt ggggatccct gaatgcgttt ttctcaccga
     4981 ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca ggagccttct
     5041 gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac ccgctggcct
     5101 gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag taccgcccta
     5161 caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac tcaacaagag
     5221 gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac attccagatg
     5281 gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccaaaca aaataccgag
     5341 tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag atttgccgta
     5401 aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt aatgctcgca
     5461 cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt ttaaaagagc
     5521 attacgcaac atcagtatga ccagcatatt tatcactatg atgctgatct ggttgctgct
     5581 ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc
     5641 agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc ctgcagcaac
     5701 tgaaacactg gcagcgctgg gccagcaagg gcaattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcgt cctggcatta cacccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccccgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctagtgga agcgctgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttct gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caata
//
LOCUS       DAJTSL010000201         6170 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTSL010000201 DAJTSL010000000
VERSION     DAJTSL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.203"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..181
                     /locus_tag="OO433_003737"
     CDS             <1..181
                     /locus_tag="OO433_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052048.1"
                     /translation="LADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLST
                     PESESGLIEQGVTCH"
     gene            192..1265
                     /locus_tag="OO433_003738"
     CDS             192..1265
                     /locus_tag="OO433_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6052049.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVITHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            1513..2553
                     /gene="pstS"
                     /locus_tag="OO433_003739"
     CDS             1513..2553
                     /gene="pstS"
                     /locus_tag="OO433_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6052050.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2640..3599
                     /gene="pstC"
                     /locus_tag="OO433_003740"
     CDS             2640..3599
                     /gene="pstC"
                     /locus_tag="OO433_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6052051.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3599..4489
                     /gene="pstA"
                     /locus_tag="OO433_003741"
     CDS             3599..4489
                     /gene="pstA"
                     /locus_tag="OO433_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6052052.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4580..5353
                     /gene="pstB"
                     /locus_tag="OO433_003742"
     CDS             4580..5353
                     /gene="pstB"
                     /locus_tag="OO433_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6052053.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5368..6093
                     /gene="phoU"
                     /locus_tag="OO433_003743"
     CDS             5368..6093
                     /gene="phoU"
                     /locus_tag="OO433_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6052054.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1536 a         1452 c         1603 g         1579 t
ORIGIN      
        1 tttggcagat tcaggaattg tcggtgaccg tggcgaactt tatctttctg gtctaccaga
       61 aaaaggacag gttacgttat cctggggaga aaacgcctca acaaaatgca tcttcaatta
      121 ttcactttcg acaccagaaa gtgagagcgg attaattgaa cagggtgtga catgtcatta
      181 aaaggatttt tatgaacaag tatatcaaac agtggtgctt tgctgtgttt atgctctcgt
      241 taagtagtgt agcacttgca gctcctaaag gtatctgtac ccctgataat ggcgtatttc
      301 acagtacgct tgatttctcg ggatatctta ttaccgcgaa cgaaaataag gttggaacga
      361 catttaacac cactgtaact aatggatcct catatcctgg acgctgtcat tgtgatactg
      421 gtaacgtagg agaattcccc tatatctatt atacgtcaaa aataaaccag gcattaactt
      481 atgctggggt tcactctaat attaattatt atgatcttaa tccgaacctt gatgttggga
      541 ttgctataga tattcttggg gttgggtatg tgaatgcacc ttttgaatat catgctaaca
      601 acccgtctgg taatacaaag tacaattgca accgcattga acctttaagt atatctagtg
      661 gggctaaagc gatagtatat ttttatatca agaagacatt tgcaggaaaa ttgattattc
      721 ctgaaacaaa gatagtgaca ttgtatggaa caattagccg tgacaccccg gtggattact
      781 cacagccgat ggctgatgtt tatattcggg gcgatattac agctccgcaa agttgcgaaa
      841 taaataattt acagccagtt tattttgatt ttaaagaaat accagctgca gatttttcat
      901 ctgttgttgg aagtgcggta ataacacata aaattactaa aacggtcact attgagtgtg
      961 aaaacctggg gatactgaat accgatgata tcagtacgtc tttttatgct accgaaccca
     1021 atacggacaa ttcaatggtc gtaacttcaa actctaacgt ggggataaaa atttacgaca
     1081 aaaataataa ggaaatcaaa gtgaacggtg gtgagttgcc aacagacatg ggtaaatcaa
     1141 cagtgtatgg tgaaaaatca ggtagtgtaa ctttttcagc tgctcctgcg agccttactg
     1201 gagctcgccc agccccgggg caatttactg caacagcgac gataactgtt gagattgtgc
     1261 gctaataggt ctgacaacga ttgttcatca catcgttgtc atctcaccca tttctttatc
     1321 acttttaaat gcatcatgac aaaaagatga catgtgggat tttcatcttc acatttaaaa
     1381 tcagtaagtt atttggtaat tcctttctgt cataaaaatg tcatattcct tacatataac
     1441 tgtcacctgt ttgtcctatt ttgcttctcg tagccaacaa acaatgcttt atgaatcctc
     1501 ccaggagaca ttatgaaagt tatgcgtacc accgtcgcaa ctgttgtcgc cgcgacctta
     1561 tcgatgagcg ctttctctgt gtttgcagaa gcaagcctga caggtgcagg tgcaaccttc
     1621 cctgcgccgg tgtatgccaa atgggctgac acttaccaga aagaaaccgg taataaagtt
     1681 aactaccagg gtatcggttc ttccggtggc gtaaaacaga ttatcgctaa taccgttgat
     1741 tttggtgcct ctgacgcgcc gctgtctgac gaaaaactgg ctcaggaagg tctgttccag
     1801 ttcccgaccg tgattggcgg cgtggtgctg gcggttaaca ttccagggct gaagtctggc
     1861 gaactggtgc tggatggtaa aaccctcggc gacatctacc tgggcaaaat caagaagtgg
     1921 gatgatgaag ccatcgccaa actgaatccg ggtctgaaac tgccttcaca aaacattgct
     1981 gtagtacgcc gcgcagatgg ctccgggact tccttcgtct tcaccagcta cctggcgaaa
     2041 gtgaacgaag agtggaaaaa caacgttggt actggctcta ccgtaaaatg gccgatcggt
     2101 ctgggcggta aaggtaacga cggtatcgcc gcgttcgttc agcgtctgcc gggtgcaatt
     2161 ggttatgttg aatatgctta cgcgaagcag aacaacctgg cgtacaccaa actgatctcc
     2221 gctgatggta aaccggttag tccgaccgaa gaaaacttcg ctaatgcagc aaaaggtgca
     2281 gactggagca aaaccttcgc tcaggatctg accaaccaga aaggcgaaga tgcatggcct
     2341 attacctcta ccacgttcat tctgatccac aaagatcaga agaaaccaga acaaggcaca
     2401 gaagtgctaa aattcttcga ctgggcgtac aaaaccgggg ctaaacaggc gaacgacctg
     2461 gattacgcca gcctgccgga tagtgtagtt gaacaggttc gcgctgcgtg gaagaccaat
     2521 attaaagaca gtagcggtaa gccgctgtac taataaaact ccaggccggg tacggtgttt
     2581 tacgccgcat ccggcattac aaaatgactt tgtaaacgcg tttaactgaa gagtaactta
     2641 tggctgcaac caagcctgct tttaacccac cgggtaaaaa gggcgacata attttcagcg
     2701 tgctggtaaa actggcggcg ctgattgtgc tattgatgtt gggtggcatt attgtctctc
     2761 tgatcatctc ctcctggccg agcattcaga aatttggtct ggctttccta tggaccaaag
     2821 agtgggatgc accgaacgat atctacgggg cgctggtgcc gatctacggt acgttggtga
     2881 cttcgtttat cgcgctgctg atcgccgtcc cggtgagttt cggtatcgcc ctgttcctga
     2941 ctgagcttgc gcctggctgg ctgaaacgcc cgctgggtat cgccattgag ctgctggcag
     3001 ccattccaag tatcgtttac ggcatgtggg gcctgtttat ctttgcgccg ctgttcgccg
     3061 tttactttca ggagccggtc ggcaatatca tgtcgaatat cccgattgtt ggcgcgctgt
     3121 tctctggccc cgcatttggt atcggtatcc tcgcggcagg cgtgatcctc gccatcatga
     3181 ttattccgta cattgcggcg gtaatgcgtg atgtgttcga acaaaccccg gtgatgatga
     3241 aagagtcggc ctacggtatt ggctgcacca cctgggaagt tatctggcgt atcgttcttc
     3301 cgttcaccaa aaatggtgtt atcggcggca tcatgctggg gctgggccgc gcgctcggtg
     3361 aaaccatggc ggtgaccttt atcatcggta acacctacca gctcgacagc gcctcgctgt
     3421 acatgccggg caatagtatc acctctgcgt tggcgaacga atttgcggaa gcggaatccg
     3481 gtctgcacgt tgccgcactg atggaactgg gcctgatcct gtttgtgatt accttcatcg
     3541 tcctcgccgc atcgaagttt atgattatgc gcctggctaa gaatgagggg gcacgctaat
     3601 ggctatggtt gaaatccaaa ccactgcggc gctggctgaa tctcgccgca aaatgcaggc
     3661 gcgtcgccgc ctcaaaaacc gtattgcgct gacgctctcg atggcgacga tggccttcgg
     3721 cctgttctgg ctgatctgga ttttaatgtc caccatcact cgcggtatcg acggtatgtc
     3781 gctggcgctg ttcactgaaa tgacgccgcc gcccaatacg gaaggtggtg gtctggcgaa
     3841 cgccctggcg ggtagcgggc tgttaatttt gtgggccacg gtattcggta cgccgctggg
     3901 cattatggcg gggatttatc tggcggaata tggtcgtaaa tcctggctgg cagaagtgat
     3961 tcgcttcatt aacgacattc tgctctccgc gccgtcgatt gtggttggtc tgtttgttta
     4021 caccatcgtg gtggcgcaga tggagcactt ctctggctgg gcgggcgtga ttgccctggc
     4081 gctgttgcag gtgccgattg ttatccgcac caccgagaac atgctgaaac tggtgccgga
     4141 cagcctgcgt gaagcggctt atgcgctggg tacaccgaag tggaagatga tctctgcgat
     4201 tacgctgaaa gcgtcggtgt ccgggatcat gaccggtatc ctgctggcga ttgcccgtat
     4261 tgctggtgaa accgcgccgc tgctgtttac cgcgctctcc aaccagttct ggagcacgga
     4321 catgatgcag ccgatcgcca acctgccggt gacgatcttt aagtttgcga tgagcccgtt
     4381 tgcggaatgg caacaactgg cctgggccgg ggtattgatt attaccctgt gcgtactgct
     4441 gctgaacatt ctagcgcgcg ttgtttttgc gaagaataaa cacggttaac actttgccgg
     4501 atgcggcgtg aacgccttat ccggcctacg gtaagcctga ttagcgaagt gcatcaggca
     4561 cgatgaggaa aagattgcaa tgagtatggt tgaaactgcc ccgagtaaaa ttcaggttcg
     4621 taatttgaac ttctactacg gcaaattcca tgccctgaaa aacatcaacc tggatatcgc
     4681 taaaaaccag gttacggcgt ttatcgggcc gtccggctgc ggtaaatcga cgctgctgcg
     4741 taccttcaac aaaatgtttg aactgtaccc ggagcagcgt gcggaaggtg aaattctgct
     4801 tgatggcgac aacatcctga ccaactctca ggatatcgca ctgctgcgtg cgaaagtggg
     4861 catggtgttc cagaaaccga cgccgtttcc gatgtccatc tacgacaaca tcgcttttgg
     4921 cgttcgtctg tttgagaagc tctcccgtgc cgacatggac gagcgcgtgc agtgggcatt
     4981 gaccaaagcc gcattgtgga acgaaaccaa agataaattg caccagagcg gttactctct
     5041 ctctggtggt cagcaacagc gtctgtgtat tgcgcgtggt atcgccattc gcccggaagt
     5101 gctgctgctc gacgaaccgt gttcggcgct cgaccctatc tctaccgggc gtattgaaga
     5161 gctgatcacc gaactgaagc aggattacac cgtggtgatc gtcacccaca acatgcagca
     5221 ggctgcgcgt tgttccgacc acacggcgtt tatgtacctg ggcgaattga ttgagttcag
     5281 caacacggac gatctgttca ccaagccagc gaagaaacaa acagaagact acatcaccgg
     5341 tcgttacggt tgattcagga gtgcgttatg gacagtctca atcttaataa acatatttcc
     5401 ggccagttca acgccgaact ggaaagtatc cgcacgcagg tgatgaccat gggcggcatg
     5461 gtggagcagc agctttctga tgcaatcacc gcgatgcata accaggacag cgatctggcg
     5521 aagcgcgtga tcgaaggcga caagaacgtc aacatgatgg aagtggcgat cgatgaagcc
     5581 tgcgtgcgca ttatcgccaa acgtcagccg acggcgagcg acctgcgact ggttatggtg
     5641 atcagtaaaa ccattgccga gctggagcgt attggcgacg tggcggacaa aatctgccgt
     5701 actgcgctgg agaaattctc ccagcagcat cagccgttgc tggtaagtct ggagtcgctg
     5761 ggccgtcata ccatccagat gctgcacgac gtgctggacg cgttcgcgcg gatggacatt
     5821 gacgaagcgg tacgtattta tcgtgaagat aaaaaagtcg atcaggaata cgaaggtatt
     5881 gttcgtcaac tgatgaccta catgatggaa gattcgcgta ccattccgag cgtacttact
     5941 gcgctgttct gcgcgcgttc tatcgaacgt attggcgacc gctgccagaa tatttgtgag
     6001 tttatcttct actacgtgaa ggggcaggat ttccgtcatg taggcggtga tgagctggat
     6061 aaactgctgg cgggaaaaga tagcgacaaa taattcacca gacaaatccc aataacttaa
     6121 ttattgggat ttgtggtaat gctgccaact tactgattta gtgtatgatg
//
LOCUS       DAJTSL010000202         6125 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTSL010000202 DAJTSL010000000
VERSION     DAJTSL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.204"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(47..628)
                     /locus_tag="OO433_003744"
     CDS             complement(47..628)
                     /locus_tag="OO433_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052055.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(675..4532)
                     /gene="sigA"
                     /locus_tag="OO433_003745"
     CDS             complement(675..4532)
                     /gene="sigA"
                     /locus_tag="OO433_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6052056.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSSAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEVGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4893..5897)
                     /locus_tag="OO433_003746"
                     /pseudo
     CDS             complement(4893..5897)
                     /locus_tag="OO433_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5894..6125
                     /locus_tag="OO433_003747"
                     /pseudo
     CDS             5894..6125
                     /locus_tag="OO433_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1529 a         1364 c         1370 g         1862 t
ORIGIN      
        1 aatgatattt cagaatatac ctcgggctaa aataccggga cagagatcag gcgtgattaa
       61 tctgcagcat ttttaataac agaagtactg agcttacttt acctttgatg atgtaatagt
      121 tatttccgtc ggtactcatt tttccctgaa ataatgtcac ggatgtttct tcctgctgat
      181 cctgcaggcg cagtcgtaag gtcatatttt taatgagctg agatccgggt ggcaaggggt
      241 atttgctgtc agatactggg cgactgagtc tgatgtggcg cgtatacagc ccgttttcgt
      301 acagggtggc ctctgttatg gtgtcagtga ccagcatgtg ggagacaatg ctgtattctt
      361 ttttaaagca cccaccagaa acggtgattg taatgataat ggtcttctcg atcgagatgt
      421 aaatacccgt ttcgttatac tgtccgtatc ggaaccatga gaactcagtt gcattccagc
      481 catgctccag aatatgtgca tttttttgcc tgatagtttc tttttttatt cgctcatgtt
      541 taaggcataa caggcagata aataatgtca gaaaaagaaa gctaataatg agagcaagag
      601 cgatatagtc tgtcacaggg taatacatat tctctccggt agaagaaggg ccgcaaacgc
      661 ggcccgggct gttatcagaa agagtaacgg aagttggcgt tgatcgcgtt atccacgttg
      721 tatttaccaa atgccgattt ctcaaactca agaccaaagc gaagattatc gcgaatttcg
      781 gcgttgagac caacattcat gagaatacga ccgtcttttt cacctttgat acgtttctca
      841 ccggacgcat cacgcagtac ggtttcaccg ttggcaaaca ggtcaaactg gtagccaagg
      901 ccggcgcggg ctgtgacttt ccagtcctta ccggagaagg atttacccac atcaacaccg
      961 gtacgcccaa tcagcggatt aaagtcctta tccttcatgg tcacgctcat tccgcgatcc
     1021 tgccagtcga accgtttacc tgatacggcc ccataaacca gttctgcctg cggctcaatc
     1081 caggagtctt ctgttacatg atagcggtag cctgcttcag cacccacata caaggaatga
     1141 gaactgtagt cttttgttcc gagcccagca aaggtcgccg agtactcatt atcatggtga
     1201 acatacttac taatcaggtc gatataggca ccagagtcaa atatagcgga agcatacaga
     1261 cctgccccga cggacttcgt cttgccactg aatgcattac tgctggcatg actgtcggta
     1321 tacgtcatag tcagaccagt gaaaaggtca agtccatcca gctcatgttt tttatccaca
     1381 ccaatctgca catgggtgta gttgtcactg tatccaccag aagctgaacc tgctccgctc
     1441 atgatgcggg cccatgcacc gacctcgcca ttggtgtcac gcagatcccc cattcgttta
     1501 ttcaggttgt tgacttctgt caggaaggct ttgtaattac cggacatata cgattttgct
     1561 gcactggaag atgctgtatt ttcagccacc ttatatccgg tcagggtcca cgtggtatgt
     1621 tcctgatcct ctttcgtgct gatcacagga gatatgttgc tgaaacctat ggcctgagta
     1681 ctggctttaa atatctcttt attgcttcct tttggggctg ttatcaggtc aacgtttaat
     1741 cctgagttat tatttcccac tttctgtata tcgaccgaca gactgttatt gcttccggtt
     1801 aactgatttt taacaatgag tttatccgat tgatttacag actggctcag atcagcccgc
     1861 ataatcacag cagtattagt ggcatccagt gtgtttactg tcacagtatg gaaacccgaa
     1921 ttctctatgt tactaaacaa caaaagactg tcgcgggttt ttaatgattg cagggctgac
     1981 tgtccgttta ctctccataa ggtgttatac atgctggctg aggcagcagg agcatcaata
     2041 cctccggtat aagaagtatc aaagccaccc agcaaacctg tagccagcgg tgtgtgtgtg
     2101 gttttgtcca ggttatcttc tttatactgg tcaacaccaa acagaataga tgcttttttg
     2161 tctgatttta tattaccatt gacccaggca taattttgag cctttaacgt ggcaccatct
     2221 tctgtcatat tatatccatc tgacatatag attgccgggc tgtatttatt attttctgta
     2281 ggggtgcctg ttaatgtcag ggttccggcc agttcaatag ctttatctac atacattttt
     2341 ccctgggcgg taacagtagc tcctttgttg ataatcaggt ctgtattaat taaggataat
     2401 gcaccagtca tagtaacagt tgatttatca ccaacagtta ttttgctgtt ttgagcactc
     2461 agactgccgt tgaaactact ttcgcctgct gaatccccgg agcgaagctg ttgtctgaat
     2521 ccaaaacctt cgccagtaat gtttcttcct gagaacatgt caatgtatgc agttttattt
     2581 cccagaacaa tttgagagtt tttagcctct atgtgtcctt ttacattagc atcccggcct
     2641 atactcaggg ttgcgttttc taaatttaat ttccggaaat taaatttacg actttcccag
     2701 tcgggctggg aaaaagaggc tatctgattg ttggacttat attccgtatt gtaacgttta
     2761 tttacagtgc tttcctggtc ctttatggca gcagaatagt ctttttgaca taaaaaagga
     2821 attggacagt tgttattgcc ttctttaaat atggcatgtt ctgtcgcatg tccttgtaat
     2881 gttaactgtg cattccggac agagatatcg tttttgatat cgactgagcc atcaaagatc
     2941 agattggcat tattgttatg ttgctgggta gtattgataa tatgatttat gcttatatta
     3001 cctgatacat tcccatggta catatatgca tcagtattag taagtgataa tgttgattgc
     3061 ttcacgtttg agttcgttac ggttgttccc gcatctgttg ctgcgatttt ctgaaatgac
     3121 tggtcatagc cgtttagatc cagtgttccg ccatttctgg taaaatagat ttcgccatta
     3181 ccgctttcgc ttaaagcgtc tttggcattt atttttaccg tacctttacc tccggccatg
     3241 taaattttat tgaaggcttt ttcagcacta aggatgacag tcccattacc tattttaagg
     3301 ttatttccct gtgctatttt tacatccaga gtaccactac ctattttatg cagattatct
     3361 ccggcaacgc ccttaacatt ccattcaacg gtggtattat tatcaatatc tatcccggca
     3421 cctttataag acttatcttt tccgtacact ttatatgtct ggttcttatc gaatataagc
     3481 ccgccaatgc caagatcggt atcctgcttg agcgttaatt cccctcctcc tgagacagac
     3541 aggtccttat cttttataat cgatgataac tcttgttttt taccatccag ctgcatagtg
     3601 tttgtgaaat tcttttttaa attgtttacc agaatattat taaataacga gtagtttgtc
     3661 caggctatat tggtactact gataaaacca actgtactgg taacaccatg gaaaacccat
     3721 ttttgttgtt ctttatcata tagataagaa ccagaaccac tatcgccaaa aattggtgag
     3781 tttttaaaag gggttctgtt ggtataaatg taactcttgt ttttccagtc cagattaaaa
     3841 agacttgcgc ttaacaagtc gggattatag ttttgtccaa acagatattg tttgccgttt
     3901 aatatcgttg aggttgttcc ggcacctgct ctgaagccaa ttatttgttc cttaccgttg
     3961 tagtttacgc catatctttt catcgcatct tcgggagaaa gtgagaagtc ggcactctcc
     4021 gtaacccctg ttgtttccac gacgaattta ttcagacgat gaaccgaaaa gtcgccagat
     4081 gatactctgt tacttgcttt atatgctgtc tgatcccatg actgtgttgt tattgcatga
     4141 tgctgtgtac cgttatgagt cgcagtaaca ctgtacgcac ctccaattga tgttgttgca
     4201 cctttgttgg aaacagcaga aaaatcaggt acaggtagtt ctggaaaatg aaatatcttt
     4261 ccatctttca tcattaattg aacatttgtt gcacccggtc tgaactctcc tctgttttga
     4321 gcaaggtcaa gataatcgcg ggtccatata ttatttatat ccagcattgc tgcaaatgct
     4381 gtttcaccat aacttccata tattgaggat aaaataatac cgaggataac tttctttctt
     4441 gatgtaccga cactaacttt ccgggtcagt tcagaaacag caactaatcc acctgtaata
     4501 tgactatatt tcagtgaata aattttattc atacgtgaac tccgatattt ctgagttgtg
     4561 gttatgggat agcatttaat tacaacgggg aactatcagt catggtacgt aactgataag
     4621 ggtataatga taatcactgt cagttatatt tgtatgacag atgtaacaaa atgaaaaaaa
     4681 agataagcac aagtgattga ttggtcagat attgatcacc tggtgtttgt attttgacct
     4741 ttgcgatcaa aatttgatct ttgttgtttc cggtcaatcc tttacaggtt ttctgtatta
     4801 tccgggggag ttaagtctgt cgatgtccga cagacttttg atgatggaaa caacatgcag
     4861 cgataatgtg catgctgttt tgcaggtgga actcagaagg tgaactggaa ctgtgcgacc
     4921 gcaccgtaag tgttgtccgt gccgatcagg cctgcaacat caaggtgaac cacatcaaac
     4981 ggtgagatcc ccaccccggc cgtgaatgca ctgccgtcgt taccggccag attctgacgg
     5041 taaccggcgc gcagctgtgc ccatttccag gcattaaatt ctgcgccaat ggcggcaaac
     5101 tgacggttgc tgtcggaggt gaaaccgctg gccggggtca gatccacatc aaatgcggtg
     5161 gtgaacatcg cgttgtgcca ggagacaccg gccgtcacct gcgggcggat cctgaaggtt
     5221 tccgtgatac cgttcacttc ttttgtctca atactacggg ggatcaggtt ctgtgcgacc
     5281 agtcccagcg tccagttgtc atccaggttc gtactggcac cgatgtcggc gttgatgcca
     5341 tttttcgtgt tgtggtagcg gtcgcctttg aaggcgctgc tgtcataatt tctgaccagc
     5401 acgttgtagt tgaacaggtc gacgcgctgg aatttggggg ggaagcccag agaccatttc
     5461 tgcccggccg tttccagttc cgtagccaga gcaataccca catcagtgat cagtgccgcg
     5521 cgggcaaagg cctgtgatgt caggcggctt ttgtccacct cctgcccggt gctgtttgcc
     5581 acctcttcca ggtaagtgag gtcagcatca gagactttac cgtcgacact gacggtgccg
     5641 tgtgatttaa ccatcagtgc ggcagagagg cgatcaccgg gaagggccgc cacagcagaa
     5701 acgcccagct gtgcggcggc atgtgtgtta cggaagtccc ggaggtgttc tttcaggcgt
     5761 gcggcagcct caggaacacc gtggttactt tcgatggcgc ggtcgaaggc cttccactca
     5821 tcactgatcc ggtcggcgtt gtcggtgata ttatccggat tggagagctg cgcgccgacg
     5881 gacggaagca ggatgctggg ctcatgcgcg acgcaaaatc cacgatgtat atatcagtac
     5941 taaaagcgcg acggcggaag aagctctgaa actaatcggt gaactgtacg ccatagagca
     6001 cgatatacgc gggttaccgg tgtctgaacg cctggcggtc aggcaaatgc agagtagacc
     6061 gctcctgact tccctgtata agctgatgca ggagaaagaa cacacgttat cgaagaaatg
     6121 tcgtc
//
LOCUS       DAJTSL010000203         6049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTSL010000203 DAJTSL010000000
VERSION     DAJTSL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.205"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..342
                     /locus_tag="OO433_003748"
     CDS             <1..342
                     /locus_tag="OO433_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052057.1"
                     /translation="NFYQGDVPSVMIRIQPENMSPLIIDISTGEHDNYIIQTLDVGTF
                     APFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTAPTHFNKTCITSDCWQILF
                     FSPDHFNNDFY"
     gene            364..1542
                     /locus_tag="OO433_003749"
                     /pseudo
     CDS             364..1542
                     /locus_tag="OO433_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
     gene            complement(1930..2306)
                     /gene="rnpB"
                     /locus_tag="OO433_003750"
     ncRNA           complement(1930..2306)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO433_003750"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2339..3484)
                     /gene="garK"
                     /locus_tag="OO433_003751"
     CDS             complement(2339..3484)
                     /gene="garK"
                     /locus_tag="OO433_003751"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6052058.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTVL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(3581..4471)
                     /gene="garR"
                     /locus_tag="OO433_003752"
     CDS             complement(3581..4471)
                     /gene="garR"
                     /locus_tag="OO433_003752"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6052059.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4501..5271)
                     /gene="garL"
                     /locus_tag="OO433_003753"
     CDS             complement(4501..5271)
                     /gene="garL"
                     /locus_tag="OO433_003753"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR6052060.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(5287..>6049)
                     /gene="garP"
                     /locus_tag="OO433_003754"
     CDS             complement(5287..>6049)
                     /gene="garP"
                     /locus_tag="OO433_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6052061.1"
                     /translation="TALWIKLIYNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAA
                     SGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVAS
                     IPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVV
                     MLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSEL
                     HSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1633 a         1580 c         1296 g         1540 t
ORIGIN      
        1 aatttctatc aaggcgatgt tccttctgtc atgatccgaa tccaaccgga aaatatgagt
       61 ccattaatca tagatatttc tacaggagaa catgataatt atatcataca aacattagat
      121 gtaggcactt ttgcaccttt tggtgaacaa tgtacttgct cagccgtcaa taaaaaagag
      181 ctggaatgta ttaaggagac gatttctaag tattgcgcaa agttcaccca aaaagaagcc
      241 atcttaacag cccctacgca ctttaataaa acctgcatta cttcagattg ttggcaaatt
      301 ctcttttttt cacccgatca ttttaataat gatttttatt gattatttat aaaggataaa
      361 ataatgttcc ctgtttcatc aattggtaat gatattagta gtgatttagt ccgtcgaaaa
      421 atgaatgacc ttcctgaaat ccccatagaa aataatctcg aagcactagc accaggtata
      481 gaaaaactaa aacagacctc tattcaaatg gtcactttac ttaacgcgtt acaaccaggt
      541 ggaaaatgta ttatcactgg tgattttcaa aaagaattag cgtacttaca aaatgtaatt
      601 ctttataatg actcgtttct tcgtctggat tttttaggtt ataacgccca aattattcaa
      661 cgatcggaca atacctgcga acttaccatt aatgaaccgt taaaaaacca ggaaatatct
      721 acaggcaata tcaatgttaa ttgtccatta aaagatattt acaatgaaat caggaggttg
      781 aacgcaacct ttagttgtgg gactggagat atagttgatc tatcctctct ggacttacgt
      841 aatgtcgatt tagattatta tgatttcaca gataaacata tggctaatac tattctaaat
      901 ccttttaaat tggattcaac aaattttact aatgccaaca tgtttcaggt taattttgtt
      961 agttcaacac aaaacgccac aatctcctgg gattatttac taaaaataac gcctgtttta
     1021 ataagtatta gcgatatgta ttctgaagaa aaaatcaagt ttgtcgaaag ttgtttaaat
     1081 gagcttggag acattaccga agaacaatta aaaattatga gatttgcaat tataaaatct
     1141 ataccaaggg caactcttac agataaatta gaaaatgaat taacaaaaga aatatataaa
     1201 agctcatcga aaatcaacaa ttgcttgaac agaattaaat taccagagat gaaagaattc
     1261 tcatcaaaaa aaaacatatg attacatcga tataatcatt gaagattatg aaaatatcaa
     1321 agaaaatgct tatctggtcg tcccccaaat taattatact atggatttaa acatagaaga
     1381 ctccagctca gaagagttaa taccctcgag aaagacgaaa attcttcgga caatggcttt
     1441 gaagtcgaag aatataacgc atatgaagca tataactcag agaatctata ttttaccaga
     1501 gaggaatata cgtatgatta cgacctttca aatgcaatat agacttaata acttcttaca
     1561 aaatctataa ttaataccgt agatattcat tcatcttacc ctgtgcacca aaagcaaaaa
     1621 ctcactgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc gctacgctta
     1681 tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg caggataagg
     1741 cgttcacgcc gcatccggca tgaataaagc gcactcaaca atctgaaacc cgccggaacg
     1801 gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg gtatagtgtc
     1861 gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa gcgggttttt
     1921 acgtaaatca ggtgaaactg accgataagc cgggttctgt cgtggacagt cattcatcta
     1981 ggccagcaat cgctcactgg ctcaagcagc ctacccgggt tcagtacggg ccgtacctta
     2041 tgaaccccta tttggccttg ctccgggtgg agtttaccgt gccacggact gttaccagcc
     2101 gcgcggtgcg ctcttaccgc accctttcac ccttacctga tcccgcttgc gcgggccatc
     2161 ggcggtttgc tctctgttgc actggtcgtg ggtttccccc ccaggcgtta cctggcaccc
     2221 tgccctatgg agcccggact ttcctcccct ccgcccgtct cccccgaaga ggacgacgac
     2281 gaagcggcga ctgtctggtc agcttcggcg cgcagtatag agggtttgcg cgcccttgtc
     2341 accccgcgtt gcgcattcca atcgccagtg tcgcggcgat attacgtgaa gcacggcaga
     2401 tattgtcata agccccgcgg aatgcttcgt ccaacgtacc tatgctggtc aatacgctga
     2461 agaccgcatc aatgccatgc tgatgtacaa cgccaacatc atcggtcagg ctacccgcaa
     2521 tgccaatcac cggtttatgg tacttcttcg ccacgtttgc gacaccaatc ggtaccttgc
     2581 cgtgaatact ctggctgtca atacgccctt caccggtgat caccagcgta caatcgtgaa
     2641 tatgttcttc cagattcagc accgtagtga cgatttcaat accacttttc agttccgcac
     2701 caagaaacgc catcagcgcc gcgcccatac cgcccgcagc tcctgcgccg gggacatctt
     2761 tcacatcaac atgcagcgct tttttaatga cctcggcata gtgagagagg ttattgtcca
     2821 gctcaacaat catcgcttca ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt
     2881 tatcgcccac cagcggattg gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc
     2941 gcggatcgag gccggaaata tcaatatcat tcagagtatt aagactaccg ccgccaaaac
     3001 caatttcatt gccgttggcg tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc
     3061 cgccatcatt tgtagcgctg ccgccaatgc cgataataat gtttgtcgca ccgctctcca
     3121 gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt cacgagtgga tcgcgttttt
     3181 ccgcaggtac cagctccagc ccactggccg ccgccatttc aataaacgcg gttttgccat
     3241 cgccggagat cccccaactg gcattcactt tctcgcccaa cggccctgta acccaggcgt
     3301 gacgttcgga accctgggtg gctgcaatca tcgcttccac cgttccttcg ccaccgtcgg
     3361 caaccggaac agaaacgtac tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg
     3421 cctgcgcaac ctcgctggca gataaacttt ctttataaga gtctggggcg attacgattt
     3481 tcatacctat gcctgttacc acatgacgcc ggagggcgtt tctcttattc ggcgtggatt
     3541 ccaggcccgg attgcaatac gccatccggg cacgacgtca ttaacgagta acttcgactt
     3601 tcgccagttt ttcgtagtag cacgccagag cgctatgatc cgccgttcct aaaccatctg
     3661 cacgcagtgc ctgcatcatc tccataaccg cagctgtgag cggcagttgc gcgccgacgc
     3721 cgtgagaagt atccagcgca ttcgccagat ccttaatatg cagatcaatg cggaagcccg
     3781 gcttgaagtt gcggtccatc accatcggcg ctttggcatc cagcacggta ctgcccgcca
     3841 gcccaccgcg aattgcctga taaaccaggt ccgggttaac gcccgcttta gttgccagcg
     3901 ttaacgcttc tgacatcgca gcaatattca gcgccacaat gacctgattt gccagtttgg
     3961 tgacgttacc tgcaccgatt tccccggtat gcaccacgga acccgccatc gctttcatca
     4021 aatcatagta tttgtcgaaa atagccttgt cgccgcccac catcactgac agcgtaccgt
     4081 cgatggcttt cggttcaccg ccgctcaccg gagcatccag catatcaatg cctttcgctt
     4141 tcagcgcttc gctgatttca cggcttgcca gcggtgcgat agaactcata tcgatcaata
     4201 ccgtacctgg cttcgcgcct tcaataatgc cattctcacc cagcgccacc tctttcacat
     4261 gaggggagtt tggcagcatg gttatgatga cgtcgcactg ttcagcgatc gctttagccg
     4321 tagacgctgt ttctgcacct gcagcaatca cgtcagcaat agcttctggg ttacggtcag
     4381 caaccaccag cgagtaacct gctttcagaa ggtttttact cattggttta cccataatcc
     4441 ccaggccaat aaaaccaact ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg
     4501 ttatttttta aaggtatcag ccagtttctg agtggcagag cggaagacgc caagatcgct
     4561 gccgacagcc acaaacgtcg cgccccattc cagataacga cgcgcatcgg cttcgaccgg
     4621 cgcgagaata ccgctgggtt tgccgtgcgc actggcacgg ttaaaaatat gctgaattgc
     4681 tttttgtaca tccggatgcg aggcattgcc gagatggccc aatgccgcag ccagatcgct
     4741 ggggccgacg aagataccgt ctacaccttc ggtagcggca atagcatcga cgttatctac
     4801 gccctgctga ctttctatct ggaccagaat agtgatgttc ttgttcgact gagcgaaata
     4861 atccgccacg gtgccaaaca tattggcgcg gtgagaaacg gagacgccgc gaatgccttc
     4921 cggcgggtaa cgagttgatg ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg
     4981 aatcaggaag ttatagaaac cgatatccag aagacgctta ataattaccg gctcgttggt
     5041 cggcactcgc actactggcg cgctggcgct gcctttcagg gccattaact gcggaataaa
     5101 cgtggaaata tcgtttggcg catgttcgcc atccagcacc agccagtcaa acccagccaa
     5161 accaagaact tcagtgctaa tcgggttaga gagtgctgac cagcaaccaa tttgtacctg
     5221 tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca tcgttattca tcgcttatac
     5281 ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca actacgaaga ggtagcagac
     5341 catcgccatc agcgctgaac atcccacgaa aaccagtgct gcattgaagg agtgcagttc
     5401 acttaccagg tagccaatca ccagtggagt gacaatggag gcaacattgc caaagacgtt
     5461 aaagacgccg ccacagaggc caacaatctc tttcggcgcg gtgtcagaaa tcaccggcca
     5521 gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc atcagcatga ccaccagcgt
     5581 ggtgttgttg gtgtagttac ataagatgat ggtggaagcc agcaacattc ccagcacaat
     5641 cggtagctta cgtgccaggg tcagggataa accgcgtttg atcagataat ccgagaagac
     5701 acctcccagc acgccgcccg caaaaccaca cagtgccgga atcgaggcga ccagacccac
     5761 tttcagaatc gacatgcctt tttcctgcac cagataaatc gggaaccagg tgaggaagaa
     5821 ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg cccaacatca tgcggttaga
     5881 gagcaattgc ttgatgtaat gcagtttggg accgcttgct gccgcgctgc ccggcttttt
     5941 gtggtccata tcgaccaccg cgccattttc agagataaac ttcagctctt ccgcagacat
     6001 acgtgggtga tctgtcgggt tataaatcaa cttgatccac agcgccgtc
//
LOCUS       DAJTSL010000204         6029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTSL010000204 DAJTSL010000000
VERSION     DAJTSL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.206"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(85..621)
                     /gene="ssb1"
                     /locus_tag="OO433_003755"
     CDS             complement(85..621)
                     /gene="ssb1"
                     /locus_tag="OO433_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6052062.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            875..3697
                     /gene="uvrA"
                     /locus_tag="OO433_003756"
     CDS             875..3697
                     /gene="uvrA"
                     /locus_tag="OO433_003756"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6052063.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3732..4088)
                     /locus_tag="OO433_003757"
     CDS             complement(3732..4088)
                     /locus_tag="OO433_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6052064.1"
                     /translation="MTISELLQYCMAKLGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLRPEEKRKLLVQL"
     gene            complement(4092..4508)
                     /locus_tag="OO433_003758"
     CDS             complement(4092..4508)
                     /locus_tag="OO433_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6052065.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLANMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMQGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4619..5332)
                     /gene="aphA"
                     /locus_tag="OO433_003759"
     CDS             complement(4619..5332)
                     /gene="aphA"
                     /locus_tag="OO433_003759"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6052066.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5734..5956
                     /locus_tag="OO433_003760"
                     /pseudo
     CDS             5734..5956
                     /locus_tag="OO433_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1485 a         1519 c         1529 g         1496 t
ORIGIN      
        1 cataacggag gcttcttttt tatggctggc agatgcttta atcatccacc ttaaaacaat
       61 ataacctatt gttttaatga caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg
      121 ctcgttagac ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa
      181 ctgattgcca ccctgcggct gctgaggctg accccaaccg ccctgcggct gaccaccacc
      241 gatattacca cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc
      301 gccaacgttc accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt
      361 acgggtacgc agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctggc
      421 cacttctgcc agtttgccga acagcacaac gcggtgccat tcagtctgtt ctttcatctc
      481 gccggtcgct ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc
      541 accatttggc atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt
      601 gtttacgcct ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta
      661 aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga
      721 aacaaacctg gccagacatt gttacacaac actccggata atgcattcca atactgtata
      781 ttcattcagg tcaatttgtg tcataattaa ccgtctgtga tcgccggtag caccatgcca
      841 ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc
      901 ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt
      961 gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg
     1021 gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga
     1081 aaagccggac gtcgatcata ttgaggggct ttctcctgcc atctcaattg agcagaaatc
     1141 gacgtctcat aacccgcgtt ctacggtggg gacaatcacc gaaatccacg actatttgcg
     1201 tttgttgttc gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc
     1261 gcaaaccgtc agccagatgg tggataacgt gctgtcgcag ccggaaggca agcgtctgat
     1321 gctactcgcg ccaatcatta aagaacgcaa aggcgaacac accaaaacgc tggagaacct
     1381 ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc
     1441 gccgaaactg gaactgcaaa agaaacacac tattgaagtg gtggttgatc gcttcaaggt
     1501 gcgtgacgat cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg agctttccgg
     1561 tggtacagcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc
     1621 caacttcgcc tgcccaattt gcggctacag tatgcgtgaa ctggagccac gactgttttc
     1681 gtttaacaac ccggcaggtg cctgcccaac ctgtgacggc cttggcgtac agcaatattt
     1741 cgatcctgac cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg cgatccgtgg
     1801 ctgggatcgc cgcaacttct attacttcca gatgctgaaa tcgctggcag atcactataa
     1861 gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta
     1921 cggttctggc aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg atacctccat
     1981 ccgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacaga
     2041 atccagtgcg gtacgtgaag aattagccaa gtttatcagc aatcgcccat gcgccagctg
     2101 cgaagggacg cgtctgcgtc gggaagcgcg ccacgtttat gtcgagaata cgccgctgcc
     2161 tgctatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactagc
     2221 tggtcagcgg gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt
     2281 cctcgttaac gtcggcctga attacctgac actttcccgc tcggcagaaa cactttccgg
     2341 cggtgaagcc cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat
     2401 gtatgtactg gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgttggg
     2461 tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc atgacgaaga
     2521 cgcaattcgc gccgctgacc atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg
     2581 tgaagtggtc gcagaaggtc cgctggaagc gatcatggcg gtgcctgaat cgttgaccgg
     2641 gcagtacatg agcggtaaac gcaagattga agtgccgaag aaacgcgttc cggcgaatcc
     2701 agaaaaagtg ctgaagctga caggtgcacg cggcaacaac ctgaaggacg tgacgctcac
     2761 gctgccagtc ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct
     2821 gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga
     2881 accggcaccg tatcgcgata ttcaggggct ggagcatttc gataaagtga tcgatatcga
     2941 ccaaagccca attggtcgta ctccacgttc taacccggcg acctataccg gcgtgtttac
     3001 acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg
     3061 acgtttcagc tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat
     3121 caaagtggag atgcacttcc tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa
     3181 acgctataac cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct
     3241 ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa
     3301 gctgcaaacg ttgatggacg ttggtctgac gtacattcgc ctggggcagt ccgcaaccac
     3361 actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac
     3421 cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca
     3481 gcaactcctc gacgtgctgc ataaactgcg cgatcagggc aataccattg tggtgattga
     3541 gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg
     3601 cagtggcggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc
     3661 ttcgcatacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg
     3721 gccttttcct ttcagagttg taccagcaat ttacgctttt cttccggtcg taaattcact
     3781 gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg
     3841 agatacacgg tgctccagtg cgctttattc agatggcggc taggacgcac atcgctgtgc
     3901 tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt
     3961 tctacttctt tcaccatcgc aaacagtaca tcttccactt tgatctgcgt cgctttccag
     4021 tcgttatgca cgctctgttc tgcgcctagt tttgccatgc aatattgtag caactccgaa
     4081 atggtcattt tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg
     4141 cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgtaac
     4201 ggcaatacaa gcgatgttcc ctgcattgag gatttgatat gagaaggcat atcgtctgct
     4261 ccctcatagt catgctcata atttccgttg tcgggaacgg tgcggaggaa aaaacgctcc
     4321 atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt
     4381 tgcagcaaca gatgcagtaa gccgatgtta acgcgcggca tattagccag ctgattcaga
     4441 atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga
     4501 taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa
     4561 ggataaaggt gtgattaaaa aaacagcatt cgggagagca tcacgctctc ccgctctgtc
     4621 agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt
     4681 aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcggcgg
     4741 taatatcatt atcagaatcg ccataaaaaa ttcggatatt tttatcctgc agccattgcg
     4801 attttgtatt ttgccctggt ttatcgcccg caaagatcac cggattcatg ttggtggcag
     4861 gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttcgtc ggactacgac
     4921 cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga
     4981 cctcttttgg aatgctgaat tcatcccagc cattgttcat tttttcccag aacacaggat
     5041 ttttcagata atcttcgctt tctggcgaga aggttttttt gccgcgccag aagcccgggc
     5101 tggaaaaaag taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga
     5161 ggctattttc aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg
     5221 caacgttagt accagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt
     5281 ttagagcgaa caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc
     5341 ctaaatagtc agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc
     5401 ataacgccag ctgcagggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga
     5461 tgattatatt taacccagta aatacgttcc tgtctttttt tgtataaaaa atgtttatta
     5521 ctcgatgtaa ggatacatta attttacgtt tattatgatg aatcctctgc aaatgagcga
     5581 aatttgatag aacatttatc tgctgtacga tttaatgaac ttaatgatac agcattacaa
     5641 aaacaatcga agtttataaa gatgattcct gattgaccag aaccctatcc tacaatggtg
     5701 attaatttta ttactcgcga tggagatgat gatatgaata tatcatatat gaatagcaac
     5761 aaaacgacat ctttacctgt tgagctagat gcactaaata ataaagatat ctcttatgcc
     5821 aaagattttt cttatgtcaa agatttcttt ttatacatag aaacactgtt aaaaattgca
     5881 aaagattttt gtagacttgg agaagaagta tcaagttcta ttgcaagtaa agtttttcac
     5941 gcatttattg atttagttaa caaaatcagg ggtaagaaag actttatgta tatttgcacg
     6001 ctttgctgtt ttgccgagga ggttaaagg
//
LOCUS       DAJTSL010000205         5815 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTSL010000205 DAJTSL010000000
VERSION     DAJTSL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.207"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..856
                     /gene="pdeC"
                     /locus_tag="OO433_003761"
     CDS             <1..856
                     /gene="pdeC"
                     /locus_tag="OO433_003761"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR6052067.1"
                     /translation="FALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYY
                     QPIIDIKTEKCIGAEALLRWPSEQEQVMNPAEFIPLAEKEGMIEQVTNYVIDNVFRDL
                     GDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEK
                     MTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIA
                     EHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLP
                     ARELTRGQ"
     gene            complement(859..1182)
                     /gene="soxS"
                     /locus_tag="OO433_003762"
     CDS             complement(859..1182)
                     /gene="soxS"
                     /locus_tag="OO433_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6052068.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1268..1732
                     /gene="soxR"
                     /locus_tag="OO433_003763"
     CDS             1268..1732
                     /gene="soxR"
                     /locus_tag="OO433_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6052069.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYRRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2278..3627
                     /gene="ghxP"
                     /locus_tag="OO433_003764"
                     /gene_synonym="yjcD"
     CDS             2278..3627
                     /gene="ghxP"
                     /locus_tag="OO433_003764"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6052070.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFGMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3779..5428
                     /locus_tag="OO433_003765"
     CDS             3779..5428
                     /locus_tag="OO433_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6052071.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5582..>5815)
                     /locus_tag="OO433_003766"
     CDS             complement(5582..>5815)
                     /locus_tag="OO433_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6052072.1"
                     /translation="MFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFF
                     EEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1258 a         1376 c         1602 g         1579 t
ORIGIN      
        1 ttttgcgttg cccgccggta ttttggggag tcttgttctg ctattactct ggctacgtat
       61 tcgacaaaac tatttgtctc ccaaacgcaa attgcaacgc gctctagaaa aacatcaact
      121 ttgtctttat taccagccaa taatcgatat caaaacagaa aaatgtatcg gcgctgaagc
      181 gttgttacgt tggcctagtg agcaggagca agtaatgaat ccggcagagt ttattccgct
      241 ggcagaaaag gaggggatga tagaacaggt aactaattat gttattgata atgtcttccg
      301 cgatctgggc gattacctgg caacacatgc agatcgctat gtttctatta acctgtcggc
      361 ctccgatttt catacgtcac ggttgatagc gcgaatcaat cagaaaacag agcaatacgc
      421 ggtgcgtccg cagcaaatta aatttgaagt gactgagcat gcatttcttg atgtcgaaaa
      481 aatgacgccg attattctgg ctttccgcca ggctggttac gaagtggcaa ttgatgattt
      541 tggtattggc tactctaact tgcataacct taaatcattg aatgtcgata ttttgaaaat
      601 cgataaatcg tttgttgaaa cgctgaccac ccacaaaacc agtcatttga ttgcggaaca
      661 catcatcgag ctggcgcaca gcctggggtt aaaaacgatc gctgaaggcg tcgaaactga
      721 ggagcaggtt aactggctgc gcaaacgcgg cgtgcgctat tgccagggat ggttttttgc
      781 gaaggcgatg ccgccgcagg tgtttatgca atggatggag caattacccg cgcgggagtt
      841 aacgcgcggg caataaaact acaggcggtg gcgataatcg ctgggagtgc gatcaaactg
      901 ccgacggaaa acgcgggaga aggtctgctg cgagacataa cccaggtcca ttgcgatatc
      961 aaaaatcgga cgctcggtgg tgcgcaactc aacggcggcc agtaacaggc gacgttggcg
     1021 aatgtaatcg ccaagcgtct gatgcgtcac cgtgcggaac attcgttgca agtaccactt
     1081 tgaatagcct gatttttttg cgactacatc aatgttaagc ggctggtcaa tatgctcgtc
     1141 aatccatgcg ataagatcct gaataatttt ctgatgggac ataaatctgc ctcttttcag
     1201 tgttcagttc gttaattcat ctgttgggga gtataattcc tcaagttaac ttgaggtaaa
     1261 gcgatttatg gaaaagaaat taccccgcat taaagcgctg ctaacccccg gcgaagtggc
     1321 gaaacgcagc ggtgtggcgg tatcggcgct gcatttctat gaaagtaaag ggttgattac
     1381 cagtatccgt aacagcggca atcagcggcg atatagacgt gatgtgttgc gatatgttgc
     1441 aattatcaaa attgctcagc gtattggcat tccgctggcg accattggtg aagcgtttgg
     1501 cgtgttgcca gaagggcata cgttaagtgc gaaagagtgg aaacagcttt cgtcccaatg
     1561 gcgagaagag ttggatcggc gcattcatac cttagtggcg ctgcgtgacg aactggacgg
     1621 atgtattggt tgtggctgcc tttcgcgcag tgattgcccg ttgcgtaacc cgggcgaccg
     1681 cttaggagaa gaaggtaccg gcgcacgctt gctggaagat gaacaaaact aaagcgccac
     1741 aagggcgctt tagtttgttt tccggtcttt gtctttctct ctatcccgct ggtacacagg
     1801 agggtttccc ccgacgtcaa cacacctcat tcgagcacgt ggtggaggtt ccggttggtg
     1861 ttgatgcttt aattgtatgt caccgacgtt tcttcgccag tgtaaaagta tactttttaa
     1921 ccgcaatatt tttgtcatct cagacgattt tttatcgcaa tcctgaacgg tatacggctc
     1981 gataacgctg caatcttgcg caccgacgat aacgtttgcg catcaattgc ctgttttttc
     2041 atcgtcaaga caataaaaga gaaaaaagca gcaaacttcg gttgaaaaag ccgctatgat
     2101 cgccggataa tcgtttgctt tttttaccac ccgttttgta tgcgcggagc taaacgtttg
     2161 cttttttgcg acgcagtaaa ttgtcgcaaa cctggagcag gaagataacg tttcgctggc
     2221 aggggattgt ccgccacgca tcttgacgaa aattaaactc tcaggggatg ttttcttatg
     2281 tctacgccat cagcgcgtac cggcggttca ctcgacgcct ggtttaaaat ttcacaacgt
     2341 ggaagcactg tccgtcagga agtggttgcc gggttaacaa cgtttctggc gatggtctac
     2401 tcggtcatcg tcgttccagg tatgttgggt aaagcaggct tcccgcctgc ggcagttttc
     2461 gttgcaacct gtctggttgc cggactcggt tctatcgtga tgggtctgtg ggctaatctg
     2521 ccgttggcga ttggttgcgc catctccctg acggcgttta ccgcattcag cctggtgctg
     2581 gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt tcctgatggg tgtgctgttt
     2641 acggtaattt ctgccacggg tatccgtagc tggattttgc gcaacttgcc gcacggtgtg
     2701 gcgcacggca cggggatcgg tatcggtctg ttcctgctgc tcattgccgc taatggtgtt
     2761 ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg cgctgggtga tttcgcgacc
     2821 ttcccggtga ttatgtcact ggtaggtctg gcggtgatca tcggcctgga aaaactgaaa
     2881 gtccctggtg gcattctgct gaccattatc ggtatctcaa ttgtcggttt gatcttcgat
     2941 cctaacgtcc atttctccgg cgttttcggc atgccttcat tgagcgatga aaacggcaat
     3001 tcactgattg gcagcctgga cattatgggc gcgctgaatc ctgtagtcct gccaagcgtt
     3061 ctggcgctgg tgatgacggc agtatttgat gccaccggaa ctatccgtgc cgtcgccggc
     3121 caggcgaacc tgctggataa agatgggcag atcatcgacg gtgggaaagc actgaccact
     3181 gactccatga gcagcgtttt ctctggcctg gtgggtgctg ctccggcagc ggtatacatc
     3241 gagtctgcgg cgggtacggc ggcgggcggt aaaaccggtt tgacggctat caccgttggc
     3301 gtgctgttcc tgctgattct gttcctctct ccgctctctt acctcgttcc ggggtatgca
     3361 acggctccgg cgctgatgta cgttggcctg ctgatgctga gcaacgtggc gaaaatcgac
     3421 tttgctgatt ttgttgatgc gatggcgggt ctggttacgg cggtattcat cgtgctgacc
     3481 tgtaacatcg taacaggcat catgatcggc ttcgcgactc tggtgattgg tcgtctggtt
     3541 tccggcgaat ggcgcaagtt gaacatcggt acggtcgtta tcgccgtggc gctggtgacc
     3601 ttctatgcgg gtggctgggc tatctaatct ttcatcggat tctgaaaacg ggtggcaatg
     3661 gctgcccgtt tttattttct ccgcacattg tgtgagcttt tgcgatattc tgaaaaaatg
     3721 agaattcagg cataacgcct ttctcagatc ataaaaaaac atcgcaaaca gggaacgcat
     3781 ggaaatcttc ttcaccatac tgataatgac cctcgtggtc tcgctgtccg gggtggtcac
     3841 tcgtgtcatg ccctttcaga tcccgcttcc gcttatgcaa atcgccatcg gtgcgctact
     3901 ggcgtggccg acgtttggtt tgcatgtgga gtttgatcct gaactctttt tagtcttgtt
     3961 tatcccgccg ttgctgttcg ctgatggctg gaaaacgccg acccgtgaat ttctcgaaca
     4021 tggtcgagag attttcggcc tcgcgctggc gctggtggtg gtcaccgtgg tcggcattgg
     4081 cttccttatt tactgggtgg tgccgggcat tccgctgatc cccgcctttg cgctggcggc
     4141 ggtactttct ccgaccgatg ctgtggcgct ctccggaatt gttggcgaag ggcgcatccc
     4201 gaaaaaaatc atgggcattt tgcagggcga agcgttgatg aatgacgcct ccggcctggt
     4261 gtcgttgaag tttgccgtgg cagtggcgat ggggacgatg atcttcaccg tcggcggtgc
     4321 gacggtcgaa tttatgaaag tagccattgg tggtattctt gccggttttg tggtgagctg
     4381 gctgtatggt cgctcgctgc gattcctcag ccgctggggc ggtgatgaac ccgcgacgca
     4441 gatcgttctg ctgttcttgc tgccattcgc ttcttatctg attgccgaac atattggcgt
     4501 ttccggcatc ctcgctgcgg ttgccgccgg gatgaccatc acccgctccg gtgtgatgcg
     4561 ccgtgcgccg ctggcaatgc gcctgcgcgc aaacagcacc tgggcgatgc tggaatttgt
     4621 ctttaacggc atggtgttcc tgctgttagg tctgcagctg ccgggtattc tggagacgtc
     4681 gctgatggcg gcagaaatcg accctaacgt cgaaatctgg atgctgttta ccgatattat
     4741 tctgatatat gcggcgctga tgctggtccg tttcggctgg ctgtggacga tgaaaaagtt
     4801 cagcaaccgc ttcctgaaga agaagccgat ggagtttggt tcgtggacca cacgagaaat
     4861 cctgatcgcg tctttcgccg gggtgcgtgg ggcgatcact ctggccggtg tgctctctat
     4921 cccgctgctc ttgccggatg gtaacgtctt cccggcgcgc tatgagctgg tgttcctggc
     4981 ggctggcgtc attctcttct cgctgtttgt cggtgtggtg atgttgccta ttctgctaca
     5041 acacattgaa gtcgcggatc attcgcaaca attgaaagag gaacgtattg cgcgagcggc
     5101 aacggcagaa gtggcgattg tggcgatcca gaaaatggag gagcgtctgg cggcggatac
     5161 cgaagagaat atcgataacc agctgcttac ggaggtcagt tctcgcgtca ttggtaacct
     5221 gcgtcgtcgc gccgatggac gtaacgacgt tgaaagttcc gtgcaggaag agaaccttga
     5281 gcgtcgcttc cgtctggcgg cattgcgttc tgaacgtgct gaactgtacc acctgcgcgc
     5341 cacgcgggag atcagcaacg aaacgctgca aaaattactg cacgatctcg atttgcttga
     5401 agcgttgcta attgaggaaa atcagtaagc cgaaagcgac gtaaccaggc ccgaggtaat
     5461 ctgaaaaagg tgcaatcttt tgcaccttta acaggttagc tcgggctaac attcatgatt
     5521 ctaaaacaaa taagttatct ctcttgtaaa gtcaattcat tggctgttct tcgacttaac
     5581 ctcaatctgc gtgttgcata atatctgcag taatcttcat agcgactgca ttaatattat
     5641 cctcagtttg tgacatgcca gtatgagagg tacaatcttc ttcaaagaat ttctgtagtg
     5701 ttaccagggt atcactatca atggggatat tttcctctcc acggagtatg gaaatgccca
     5761 ttccctggac gctagcctca tcaaaattaa cccgatagag acgggtatta aacat
//
LOCUS       DAJTSL010000206         5747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTSL010000206 DAJTSL010000000
VERSION     DAJTSL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.208"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..385)
                     /locus_tag="OO433_003767"
                     /pseudo
     CDS             complement(1..385)
                     /locus_tag="OO433_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(571..3879)
                     /gene="icsA"
                     /locus_tag="OO433_003768"
                     /gene_synonym="virG"
     CDS             complement(571..3879)
                     /gene="icsA"
                     /locus_tag="OO433_003768"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6052073.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEITGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTTHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISHSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4408..5610
                     /gene="virA"
                     /locus_tag="OO433_003769"
     CDS             4408..5610
                     /gene="virA"
                     /locus_tag="OO433_003769"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6052074.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQLNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1778 a         1299 c          949 g         1721 t
ORIGIN      
        1 tacgcttccc catgtgattt gcctcgccac ttcaccgggc tcaggacccc gtaggatctt
       61 caccgaaaaa aatgcatcac cagttgcttt attatttgtt ttaggtagac ctgttactaa
      121 tttataccta ctctgaccaa gtctggataa gaaaggcaca cctgcacatt tctacttgga
      181 aatgttttct tttaccattt tgctcaaaaa tttcaacatc caatattcct cgtacagaac
      241 tactcaagtc ctgaatagaa aaagttccag ctggtacagt agagtcatat attgctcgac
      301 cttgttgaga tacaacaaca cgtgcattag tttctgcaat accagtaatc tgaggcgcat
      361 aaccacgaat atttggcggt agcattcgtt catcgctttc taatgcaatt cctgtgtaac
      421 gccaagcaac agcgcattct ggcgcatgat ctggtaaaca cgtttggcat tgatcgcagg
      481 cataccatca tgtgcacaaa aacccaccga gcttttacac ggtgggtccc agagaaatgc
      541 aggacatcaa cacgccctgc atttttatta tcagaaggta tatttcacac ccaaaatacc
      601 ttgggtgtct ctgtaactgt tattacccaa ttgctgagat acgtttcccc agatgcttag
      661 gttatctgca agacgccccc ccacaccagt tttgagttca atcacattac ggttgcctat
      721 ctggtgattt gacatgccat tcataattac accatattgc tttgagctcc atttccaatt
      781 cacttccaaa aatggttcga acttacgaag agtctcctta tcgatacaac tatgactaat
      841 taaggatgta cgcaatccca attttgcttg aatattattg accccagagg atgtgaccat
      901 agtgccattg tgttcaatat agtcgtttgc ttttacccca tgcctagtta tctggacttg
      961 tggattgaga tacaatgcgt tgtcaatatt attatgagta gtccagcgta tggttgggta
     1021 gatatagcca gcttccaaag ctcctgttat tcctttgctg gaatatttct caccagttaa
     1081 cccatctcct ttgactgtat tattaaacca accgtactgc aaccaagcat ccatatatag
     1141 acctgttcgt tctttttcat cctgaaacca agaactgtac aaaccggcag tataaccgct
     1201 cacagttcct cgtgaactat aactagtata cttattatga gttttgctat cctgatatcc
     1261 ataagctccc ataataccca catgcaagcc atcatgatca gtgaagtttg tcttcaaaat
     1321 atctccacca atctgataaa ccatcctgtt agctgtggtt tttaattgtc tatcagctaa
     1381 attatgctca ttatgtccac caacagtacg aatccacatg gtggtttcgc tgagctgacc
     1441 tgtagtagga tcaacaatag tgctaccgtg tcggtcatag agagaaggac taaacatcgt
     1501 gttcattgct atcaggttag caatataact gccagcttca gggcgccatg tgtgaatact
     1561 accttcaccc aaattaagtg aagatacaag ctgtgaacta gctcggcgac tactcatttg
     1621 agtagactct tgattgctca tctgttttga ttcttgatta tccatctgac tagttagata
     1681 ccacttattg gtattcagtc cagatacagt gccctgtttc aggcgatagt cataactacc
     1741 agcaacaata cggcctttct gaataaaagc attcttatca gaagaatctg tcgaaataat
     1801 ttgaacacct tcaagagttt tatctccaga gcctccctca tttgtcactg caacataggt
     1861 aataccagac gcatggcctg taatttccag ccggtcagtt ttactgttgt cattgccgag
     1921 aaccgcgccc aaagataata ttccgccctt tccatgccaa ttacctttct gaacataggt
     1981 ctgaccgaca tttgacgaac tattattgag aataacatga ccgtttttct ccagagtcat
     2041 gttacctgtg acaatgacgg ggtcaggcaa aaaaggggca ttaatattat tgattagcac
     2101 agtcccacta tttaataaag tgttaacagt ttggttcatg cctgaaaaat tcaaggtagc
     2161 acctttatta acaataacac cagcggtacg tgtcatagct tcaactgtcc ccatttttag
     2221 aatgcctgca ttgatgttgg tgaaaccaga gtaggtattt ttttccgcca aaatgagagt
     2281 tccctcccct gattttgtta gggattttcc gtcccagcta tttatattat gatggctgag
     2341 attatctgcc agaatagtac taacagtaag ttcagcgttt tctttcagat tgaactctcc
     2401 atgactgtcg ccatctgtat cattccagct caaaccgtag atcaatttat tctggtcact
     2461 gatatagctt tttgtatagt caatagcacc agtagtgagt aacggattga tgatagatat
     2521 attagaaaca ctgaaacctg ttatcccttt atctgcactt agcagtttat agtccttctg
     2581 cgcccagtta tttataatag cttccacttt tacattgttg ccaaatgaca tatttccaga
     2641 agcttccaga ctactgattc cagctaaatt taaagaactg ttctcaccaa aagaaacgtc
     2701 acctgaaaca gtgaatttgt tacctgatag tgaaacagtt gtacaatcac cagcacataa
     2761 atcaccttca atagttggtg tggtcccagt gtaatcattg ttgataattt tcagaatgga
     2821 tgaattgtta acctgtacat caccagtaat gacagaacct tcatggagta ttaatgaatt
     2881 gtttccacca gtgatgtgga tagcattacc tgctacattt tgtgttccat catcttcttt
     2941 acctgaaatt atatatccgc tattagcaat ggtcatattg ctaccattta tagcatcccc
     3001 ccaatgtcca ccattaccgc ctaaaatagc tccattgttg attatagaca ggttatctcc
     3061 tgtgatagca ttaccaccgt agccatcata accataaccg taagcagcac ctccgttgcc
     3121 tccagcgaac gtgcccttat tgattacaga gaggctgctt cctgtgatgg catcacctcc
     3181 attaccgcca tcaccctcac cataattgtt acctccatta ccaccggaaa tagtcccttt
     3241 gttgattatg ggtagattga ctcctgtgac ggcatcacct ccattccccc catcactatc
     3301 actataacta tcgccaccat gtccacctga aataatttct ccatttatta taaacagatt
     3361 gtctcctgta acagcctcac caccatcacc gttatggtca gcaccgctac cgccgctacc
     3421 accaagaatc atgccttgat tgattataga caagtcagat ccggtaatag agtcaccacc
     3481 attaccgcca caactattac catcattatt atcaccagca ccaccatgac ctgcgctaat
     3541 cataatatca gagttaatcc gtagcttagt tctagatgca tgagaggggt tggaagaagt
     3601 tatataccac gcatccattc catcttcacc agctcccaag ggagaaagtc catcaacagg
     3661 tgttaataat ttttcataat tgtcctctga aaaatgaagt tcttgagtac ccgaaagagg
     3721 aatagcaaaa gctattggcc ccccgagcaa cagggatgca ccaacaacaa aaggagaaaa
     3781 agacaatttg catgtttttt cctttaccgt aggtaattct ccggcccccc cctgtgaaca
     3841 ttgggtcata ttacaaaaaa atttgtgaat ttgattcatg cactatatta tcagtaagtg
     3901 gttgataaac ccctaaaaag attccgtttt atccggaata aagggacgat atatgcaaaa
     3961 acatattaaa caaagcctca accaaaacat gagagctgtg ttatatatca atgcaacggg
     4021 agaaatttac ctgtgtcttg ctttgaaaaa aagaggggga aagctatatg ttcgataata
     4081 aattaataat tttactatga tagtctgctt cagaccgcaa cttttcagtt ctttctgtac
     4141 gcttgcccaa agtttaacca ctttgttttt ctatacaccc aatggttcta tctgcccctt
     4201 gatgttaatc cccaaagcca ataacatgaa tgtattcata tcacaacttt cctaccagac
     4261 tctccaatgc aataactgca taattgtttt tagtggaaat gtatcgtttt cttaaagaga
     4321 agaataacat tccattttat tatgttccca atttaattat attaatatat attggcatca
     4381 ataggaaaat acatcaggag aaatcaaatg cagacatcaa acataactaa ccatgaaaga
     4441 aatgactcat catggatgtc aactgtcaaa tcaactacag aagtaagttg gaataaattg
     4501 agtttttgtg acgttttact gaaaataata actttcggaa tatattctcc acacgaaact
     4561 ctagccgaaa aatatagcga aaaaaaacta atggatagtt tctcaccatc actatcacaa
     4621 gataaaatgg acggagagtt tgcccacgca aacatagatg gcatttcaat acggctatgc
     4681 cttaacaaag gcatatgttc ggttttttat cttgatggag ataaaataca atccactcaa
     4741 ctttcatcaa aagaatataa taacttgtta agttcactcc cccccaaaca attcaactta
     4801 ggaaaagtac atactattac agctccggta tcagggaatt ttaaaactca caagcctgca
     4861 ccagaagtta ttgaaacagc cattaattgc tgtacatcga taataccaaa cgatgattac
     4921 tttcccgtta aagacactga ttttaatagt gtttggcatg atatttacag agatattcga
     4981 gcatctgaca gcaatagtac aaaaatatat ttcaacaata ttgaaatacc actaaaatta
     5041 attgcagatc taattaatga gttaggaatt aatgaattca ttgatagcaa aaaagagctt
     5101 caaatgctaa gttacaacca attaaataaa ataatcaatt caaacttccc ccaacaagac
     5161 ctatgttttc aaacagaaaa acttttgttt acttcactct ttcaagaccc tgcatttata
     5221 tccgcactaa catctgcatt ctggcaatct cttcacatca cgtcttcctc tgtggaacac
     5281 atatatgccc aaattatgtc cgaaaacata gaaaacaggc ttaatttcat gcctgaacaa
     5341 cgagttatta acaattgtgg acatattata aaaatcaacg cagttgtccc taaaaacgac
     5401 actgcaatct ctgcctctgg ggggagggct tacgaagtta gctcatcaat attaccatcg
     5461 cacataacat gtaatggtgt tggtattaat aaaatagaaa caagctatct cgttcatgct
     5521 ggaacacttc caagcagtga agggcttcgc aatgctatcc ctccagaaag tcgtcaagta
     5581 tcatttgcca tcatatctcc tgatgtttaa gccaatatta tattggctgc cagactgtaa
     5641 aaaaacgttc ttcagaacag ctctataaac tattaaacca aagctaaaag gatggggacc
     5701 aatagaatct cagcccatcc tttttatatc attatgaacc gccccgg
//
LOCUS       DAJTSL010000207         5736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTSL010000207 DAJTSL010000000
VERSION     DAJTSL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.209"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(14..382)
                     /locus_tag="OO433_003770"
     CDS             complement(14..382)
                     /locus_tag="OO433_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052075.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            908..1603
                     /gene="minC"
                     /locus_tag="OO433_003771"
     CDS             908..1603
                     /gene="minC"
                     /locus_tag="OO433_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6052076.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1627..2439
                     /gene="minD"
                     /locus_tag="OO433_003772"
     CDS             1627..2439
                     /gene="minD"
                     /locus_tag="OO433_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6052077.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2443..2709
                     /gene="minE"
                     /locus_tag="OO433_003773"
     CDS             2443..2709
                     /gene="minE"
                     /locus_tag="OO433_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6052078.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(3188..3307)
                     /locus_tag="OO433_003774"
     CDS             complement(3188..3307)
                     /locus_tag="OO433_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6052079.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3268..3453)
                     /locus_tag="OO433_003775"
     CDS             complement(3268..3453)
                     /locus_tag="OO433_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052080.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            3554..3727
                     /locus_tag="OO433_003776"
     CDS             3554..3727
                     /locus_tag="OO433_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052081.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVRATYLIPDI"
     gene            3729..4073
                     /locus_tag="OO433_003777"
     CDS             3729..4073
                     /locus_tag="OO433_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6052082.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4077..4412
                     /locus_tag="OO433_003778"
     CDS             4077..4412
                     /locus_tag="OO433_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6052083.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4469..>5736)
                     /locus_tag="OO433_003779"
     CDS             complement(4469..>5736)
                     /locus_tag="OO433_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6052084.1"
                     /translation="LIVEGNTSGSTTVYVKNAGGSGAATLNGIELITVNGDESPAEAF
                     RQGDARIAAGAFEYQLKQQGKNWYLTSYQSVNEEDNSSEGNSEPTETPTPVLRPEAGS
                     YVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRT
                     TSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYCAG
                     LYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALN
                     KSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRT
                     QEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGNLNPA
                     ASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT         1570 a         1338 c         1325 g         1503 t
ORIGIN      
        1 tgtaaacaag gcatcactca cgacacataa ataacatctt tgtggtgatt tgattgattt
       61 ttccactccg cttaccatcc acaccaaaat aacgatcatc acgacataat ttttctttaa
      121 catcgcaaaa tacaccgtta gcgaatgtaa attcgctggg atcgtagcct tgtaatgatt
      181 gtaagttttc agcggctttt tcgccgagat atttccttgt taactctggg gaaataccat
      241 ctgagttcac acaaatatac gggtcgcaaa caacgccgca cgccggagat tgaagtccgc
      301 cagcgtcatg tccagacgcc attgatgaga acatcatccc cacacctgac aatccgatat
      361 aaaaaatacg catcatattc atagcacatc ctgataatcc aaccgtacaa cacttatgct
      421 catttcatca tcaatccgta atcaaaaccg ctgacctgga cgagttacga tgacctgaca
      481 gaagtatcag acgtcttctg cggtttctat gattatgata gagcaattct atggcggata
      541 ttaagataac gggtatctgt tctttaatga tgctgcaaaa aatggaaagt taagaaagcc
      601 tggcgtggtg aaaaaataca cttgcctcaa tactggatat ttaaacaggt ctggagatgc
      661 tgtcacgatt agggtgcgga aaaaggaagc gtaaataaga acaatcaaaa gtacgacggc
      721 aatgggttga ttgacaaggg tattttttaa gctatgaatc agcgccattt atcacagaat
      781 agacttttac tctgaataaa tgggagggtg acttgcctca atataatcca gactataaca
      841 tgccttatag tcttcggaac atcatcgcgc gctggcgatg attaatagct aattgagtaa
      901 ggccaggatg tcaaacacgc caatcgagct taaaggcagt agcttcactt tatctgtggt
      961 tcatctgcat gaggcagaac ctaaggttat ccatcaggcg ctggaagaca aaatcgctca
     1021 ggcccccgca tttttaaaac atgcccccgt tgtactcaac gtcagtgcac tggaagaccc
     1081 ggtaaactgg tcagcgatgc ataaggcggt gtcggcaacc ggtttgcggg ttattggcgt
     1141 aagtggctgc aaagatgcgc aacttaaagc cgaaattgaa aagatggggc tgcctatcct
     1201 gacggaagga aaggaaaaag cgccgcgtcc agctcccgca ccacaggctc cagcgcaaaa
     1261 tacaacgccg gtcacaaaaa cgcgtttaat agataccccg gtgcgttccg gtcagcgtat
     1321 ttatgctcca caatgtgatc tgattgttac aagccacgtt agcgctgggg ccgaattgat
     1381 tgccgatggg aacattcatg tctatggcat gatgcgcggt cgtgcgcttg caggggcaag
     1441 tggtgaccgg gaaacgcaaa tattttgtac gaacctgatg gcggaactgg tgtccatcgc
     1501 aggtgaatac tggctgagtg atcaaatccc agcagaattt tatggcaaag cggcgcgact
     1561 gcagttagtc gaaaacgctt tgaccgttca accgttaaat tgatcccttt ttaacaagga
     1621 atttctatgg cacgcattat tgttgttact tcgggcaaag ggggtgttgg taagacaacc
     1681 tccagcgcgg ccatcgccac tggtttggcc cagaagggaa agaaaactgt cgtgatagat
     1741 tttgatatcg gcctgcgtaa tctcgacctg attatgggtt gtgaacgccg ggtcgtttac
     1801 gatttcgtca acgtcattca gggcgatgca acgctaaatc aggcgttaat taaagataag
     1861 cgtactgaaa atctctatat tcttccggca tcgcaaacac gcgataaaga tgccctcacc
     1921 cgtgaagggg tcgccaaagt tcttgatgat ctgaaagcga tggattttga atttatcgtt
     1981 tgtgactccc cggcagggat tgaaaccggt gcgttaatgg cactctattt tgcagacgaa
     2041 gccattatta ccaccaaccc ggaagtctcc tcagtacgcg attctgaccg tattttaggc
     2101 attctggcgt cgaaatcacg ccgcgcagaa aatggcgaag agcctattaa agagcacctg
     2161 ctgttaacgc gctataaccc aggccgcgta agcagaggtg acatgctgag catggaagat
     2221 gtgctggaga tcctgcgcat caaactcgtc ggcgtgatcc cagaggatca atcagtgttg
     2281 cgcgcctcta accagggtga accggtcatt ctcgacatta acgccgatgc gggtaaagcc
     2341 tacgcagata ccgtagaacg tctgttggga gaagaacgtc ctttccgctt cattgaagaa
     2401 gagaagaaag gcttcctcaa acgcttgttc ggaggataag ttatggcatt actcgatttc
     2461 tttctctcgc ggaagaaaaa cacagccaac attgcaaaag aacggctgca gattattgtt
     2521 gctgaacgcc gtcgcagcga tgcagaaccg cattatctgc cgcagttgcg taaagatatt
     2581 cttgaggtca tttgtaaata cgtacaaatt gatcctgaga tggtaaccgt acagcttgag
     2641 caaaaagatg gcgatatttc tattcttgag ctgaacgtga ccttaccgga agcagaagag
     2701 ctgaaataag cccgctgtaa aaacgcattt atcttcaagg cagagtcatt tctgccttga
     2761 gtttttcatc cctctcatcc acgttgtggt aaagcggcga gtattcttgc tgatactcct
     2821 cattgctatt tccacctccc cctttacacc ttaaggctgt tcccacgcac agaacaaaat
     2881 gtttatgcct ggttgagtaa ataacctcat tgttagtgtg gatacactgg gggatgataa
     2941 ccccgcaatt tcattattca ttgttctccc ataggatgag gtttcccgcc gtaactggcg
     3001 ggcttttttt gccccagaat tttccctttt agccccctgt aaacgaaaac tgcgccgaag
     3061 cgcagtttaa tcgttgatgg tcagaacgac cagttcacga tatatactgc gggtatcgac
     3121 tgatttcgat tccatgccat tggataaatg atagtagcgt ctaagtgacc taagctctgc
     3181 tgcaacatca gcccgcaggc accagaccag gggattcatc ctgaagagac agcgcaggtg
     3241 tattgtgttc accactcacc aaagacatca tgatgaaatg atgatatccc gcataagaaa
     3301 gaggcatttt ttaaacgcag tgcgctgaag tgtagttgga taaaaaatca ctcttttcag
     3361 ataatactgt cgtacgctta tctttcgaca atgaggccgt cggcaaaata aaatgactaa
     3421 tgtaatcgtt gctgatgaat atcttctgct cacataaaaa tcacacaata atattgataa
     3481 atcagattat cttaccttta caacattgcg ttaacagtca atgacacttc ttgaataaaa
     3541 atattgccaa tcaatgagtt attcctcatt taagattatt ctcattcacg taaaaaacat
     3601 aattcctatc atcacagcca cgctcatttt gaattatctg aataatagcg agcgttcttt
     3661 agttaagcag atcttaattg aagacgaaat tattgtgcgt gcaacttact taataccgga
     3721 catctaagtt gaagaaaaaa atacttgcct tcgggcttat ctcagcattg ttctgctcca
     3781 cgccagcgat ggcggatatg aatcgcacca cgaaaggtgc attgctaggc gcaggcgttg
     3841 gtttacttac tggcaatggt gttaatggcg tactgaaagg tgcagctgtt ggcgctggtg
     3901 ttggtgcagt aacagaaaaa ggccgcgacg gtaaaaatgc gcgtaaaggt gcaaaagtag
     3961 gtgccgccgt tggtgctgtc actggcgttc tgaccggcaa tggcctcgaa ggggcgatta
     4021 aaggtgccgt gattggcggt acgggtggtg caattctggg taaaatgaaa taaggaatga
     4081 tgatgaaaaa atttgcttta ctggcgggcc tgtttgtttt cgcaccaatg acctgggcgc
     4141 aggactacaa tattaaaaac ggtttaccgt cagaaaccta tattacttgc gcagaagcca
     4201 acgaaatggc aaaaactgac agcgcacagg ttgcagaaat tgttgcggta atgggtaatg
     4261 ccagcgttgc cagccgtgat ttaaaaattg agcaatctcc ggaactgagc gccaaagtgg
     4321 tagaaaaact gaatcaggtt tgcgctaaag acccgcaaat gctgttgatc acggcaattg
     4381 atgacaccat gcgtgctatc ggcaaaaaat aatatttcat atgtaccgcg tcgatgaata
     4441 cgacgcggtt ttttagctaa cggcgggatc agaacttata tttcaggccc accatcactg
     4501 cggtgtcatt gtagccatta tcacccagct gcacgcccac attcccccag acgctggccg
     4561 ctggattcag gttgccattt acgccaagtt taatttcccc caaattactc gccccatctt
     4621 gttcgacttt cacaccgttc attgagacag caaaatcttt actgttatgg atccagttca
     4681 tttcaacaaa aggttcaaag tcgtcaccgt gcggaccgct gtttttctcc tgagtacgaa
     4741 taaaggtgcg gaaaccaagt cgggtctgga tgttgttatt tgcgtcattc tcaatacgtg
     4801 agccattggc ttcggtgtgc gcattatgat cgacgcccat ccagatagcc tgtgcctgtg
     4861 gctggaatat ccacgtgtat ttcgccgctt ccagaccaaa ggatttattc agggcaaagc
     4921 cgtaacccgc ttccagcgag acggttgcgc ctttcgcact ataggattca taggctaatt
     4981 catccccttt caccgagttt ttaaaccagc tatattgcgc ccaggcgtca atgtatgcgc
     5041 cttttttact gatgtcatcg gcaaaccaag tggcatacag ccctgcgcaa tagcctctga
     5101 cactcccttt cgaacgataa tccgacacgc tggaatgagt tgagttgtag tcgcgggcat
     5161 aaccagccat cactcccaaa cgccaactgt cactgtcggt aaaaccaccg gttaacagat
     5221 cggcccctaa ctgcgagacg tagcgatgcg aggtcgttct caactgtccg ttgctgtcac
     5281 gccaggcatt atgcccgcca atttgacgta gccaaagtcg gcttgaacgc tcctgttcag
     5341 ttacaggatc gatgtagcgc atttcacccg cacggtcgtt cagacgcata acaaaaagag
     5401 tgttagcggc tgccaggttg gcgacgtaac ttccagcttc ggggcgcagg acgggggtag
     5461 gcgtttctgt tggttctgaa tttccctctg agctgttatc ctcctcattc actgactgat
     5521 aacttgtcaa ataccagttt ttgccttgtt gttttagttg atattcgaaa gctccagctg
     5581 caatccttgc atcaccttgt ctgaaggctt ctgctggaga ttcatcgcca ttcacagtta
     5641 tgagttcgat cccgtttagc gtggccgcgc cgcttcctcc agcatttttc acatagacgg
     5701 tagttgaacc cgaagtatta ccctcaacaa taagtc
//
LOCUS       DAJTSL010000208         5734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTSL010000208 DAJTSL010000000
VERSION     DAJTSL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.210"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(97..2199)
                     /locus_tag="OO433_003780"
     CDS             complement(97..2199)
                     /locus_tag="OO433_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6052085.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPSDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAVGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2441..3502
                     /locus_tag="OO433_003781"
     CDS             2441..3502
                     /locus_tag="OO433_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR6052086.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3615..5114)
                     /gene="ansP"
                     /locus_tag="OO433_003782"
     CDS             complement(3615..5114)
                     /gene="ansP"
                     /locus_tag="OO433_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR6052087.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5381..>5734
                     /locus_tag="OO433_003783"
     CDS             5381..>5734
                     /locus_tag="OO433_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6052088.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLTLENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWA"
BASE COUNT         1516 a         1482 c         1370 g         1366 t
ORIGIN      
        1 ttgtcttgct gaaattatgc aacaaaatat tgctatttta taccagcaat acaatcgtta
       61 aagtaaatgc ccggcaaata ccgggcatca aggtaattac tcaaatctcc acgcaatatt
      121 catgccgaca ccataatttc gtccgggcga aggttcgtaa tatcgcccgt ttgactcatt
      181 gacaatgaca gaaccaacgt attctttatc gaataaatta tcgacacgac caaataaatc
      241 cacagttaaa ttgtggtaat tgtatttata cccggtgaat aagccgacga gagtataaga
      301 cggcgctttt gccgtatttt catcatctgc cataatatcg cccatataac gcgcttctgt
      361 gcctgcatac caaccatctt ccggtacata acctatcgac gcaaagccca tattacgggc
      421 gatccctggc atccgattac cgttacaatc ctgttcattg caaacattgc tgcgataggt
      481 cgcatcaagc caggtccagg acgcgtttac gcgaaaatct cctgcgaaac gttgatccca
      541 tgccagttca gcgccttgac gacgggtctt tccggcattt ttgtaagtcg tacgcccacc
      601 gctactgcta tcgacaacaa tttcatcatc agtgtcggtc tgaaacaatg cgagactaag
      661 cagcccatca ccaatacgcg ttttactgcc gatctcaatt gtatcgttgg tggatggttt
      721 taaaccaaag ttcataccgc tttgcccatc agcacgataa gacagctcat taatcgtcgg
      781 cgtttcaaaa cctcgcccga ctgccagata gatattccag gcatcggtca ttgcatattt
      841 taacgaaccg gcaggtagcc atttatgata actggcatca ccgctgtcat cgccgttacc
      901 cggagtaacg taatggtcgt tggaatcaaa ccacacggag ctgtagcgca cgccagcatc
      961 cagcgacagt ttttcgctca gctgccactg cgtctgtaaa tagggatcga tgttccacat
     1021 cagattgcgt tcgtcgcgac gcaactcacc tttttgcccg tactccggca tgccgctatt
     1081 caggcggaag ttattgtagc ccttgcggtt ttcactcatg ttttcgtagt tcaggccggt
     1141 agtgaacgtg accggaacgc ccagttcacc acggtgtgtc cagcggctgt ctatcccctg
     1201 gtaatggcgt tgcagggtaa tcacgccgcc cgcatgtgac gggttaagtt gtggtgccat
     1261 gggtattgac tggtactggg tcgtttctcg ctctccggca tacatcatca cactcatatc
     1321 atcttgcgcg ctcaggctac gctcatagcg caaccctgcc tgagtttgct tgatggtttt
     1381 tcgcgtgtcg tactgttctg cacgaggcgc ttgttgtgga ttagccttcc attctgcttt
     1441 ggttagccca cctgggtcat ctgctttgat atccacacta ttgaaaatca gacttaattt
     1501 gctggcttca tcaatgcgta cgcccagttt ggcattggct aaatttttct gtgcgccact
     1561 atggtcacga tagccgtggg tcgtaaaacg cgtggttgag acggtgtaat cgacatcgct
     1621 aggctgtgtg ccgtctcccg ttgcgcccgt tgctttcagc ccatagcgcc agctgccaaa
     1681 actgccgtag taactactgg cttcaatggt tggtggctgt tgtccggtct gggtggtgac
     1741 attcattacc ccaccagacg cgttgccata cagggcagag aaggggccac gcagcacttc
     1801 cacattttgc acactgctta aatcgatgtt ggatgtttgc ccttgcccgt cgggcatggt
     1861 ggcgggaata ccgtccacat acaggcgaat accgcgaata ccgtaagtgg agcgggagcc
     1921 aaatccgcga atcgacagct gtaaatcttg cgcatagttc tgccggtttt gtacctgcaa
     1981 accaggcacg ccggtcagtg attcggacaa gttaatgcgc ggtgttgcca ggcgcatctc
     2041 ctcgccatcc accacgctta ctgctgctgg ggtatccagt tctgaaacca cctgcggtgc
     2101 ggcactgaca atcatagtct gttcatcagc ggcaaaaaca acgggggaaa ggacaagcag
     2161 tgcgggcaaa acggtctgtc ggacggaaaa aatcttcatg aaaaaagcca ggttaagaat
     2221 gggaaaacgc tgtcatggta ataaaattgt aaacttatgc aaaacgaaac ggcacgatac
     2281 gttaattaat tgagaaaatt gtagtcgtaa cggcaagaaa tgctccacat ttgagaaaat
     2341 aatgattacc attcccattt ataacaagag cgtgacgatg attacgctta gcgaagcatt
     2401 gtgaagcagc aaaaatattg gttcatcaaa gggagtcgtc atgcatttac gtcatctgtt
     2461 ttcatcgcgc ctgcgtggtt cattactgtt aggttcattg cttgttgctt catcattcag
     2521 tacgcaggcc gcagaagaaa tgctgcgtaa agcggtaggt aaaggtgcct acgaaatggc
     2581 ttatagccag caagaaaacg cgctgtggct cgccacttcg caaagccgca aactggataa
     2641 aggtggcgtg gtttatcgtc ttgatccggt cactctggaa gtgacgcagg cgatccataa
     2701 cgatctcaag ccgtttggtg ccaccatcaa taacacgact cagacgttgt ggtttggtaa
     2761 caccgtaaac agcgcggtca cggcgataga tgccaaaacg ggcgaagtga aaggccgtct
     2821 ggtgctggat gatcgtaagc gcacggaaga ggtgcgcccg ctgcaaccgc gtgagctggt
     2881 agctgatgat gccacgaaca ccgtttacat cagtggtatt ggtaaagaga gcgtgatttg
     2941 ggtcgttgat ggcgagaata tcaaactgaa aaccgccatc cagaacaccg gtaaaatgag
     3001 taccggtctg gcgcttgata gcaaaggcaa acgtctttac accactaacg ctgacggcga
     3061 attgattacc atcgacaccg ccgacaataa aatcctcagc cgtaaaaagc tgctggatga
     3121 cggcaaagag cacttcttta tcaacatcag ccttgatacc gccaggcagc gtgcatttat
     3181 caccgattct aaagcggcag aagtgttagt ggtcgatacc cgtaatggca atattctggc
     3241 gaaggttgcg gcaccggaat cactggctgt gctgtttaac cccgcgcgta atgaagccta
     3301 cgtaacgcat cgtcaggcag gtaaagtcag tgtgattgac gcgaaaagct ataaagtggt
     3361 gaaaacgttc gatacgccga ctcatccaaa cagcctggcg ctgtctgccg atggcaaaac
     3421 gctgtatgtc agtgtgaaac aaaaatccac taaacagcag gaagctaccc agccagacga
     3481 tgtgattcgt attgcgctgt aatagatatg gcgagggtga aaacggtgag acttgcctgc
     3541 cttttatcac ggcgtatccg gcactttcgt cagtaatctg aatttgcccg gcacgtagcc
     3601 gggcaatttt gcaattaact cgccgtttca ggcttaaaca caatttcctg tttttcttca
     3661 tcttcctcga cgactggcgc agtgctgtga atttcagcaa cgcgtttgcg cacaccaaac
     3721 cagcctataa ccagcagaat accgataatc ggcagcgccg cgatagtata agtcccgttc
     3781 gggtaatcga acgccatcag cacaaggaca ctcagtaaaa acagtaatgt cagccaggaa
     3841 gtgaagggcg cgccaggcag tttaaaactg acatccgctg ctttgccttc tttaatcgct
     3901 ttacgcaggc gcatctggca cacgatgata aacgcccatg aagcgatgat tcccagcgac
     3961 gcgaagttca acacaatctc aaatacgcgc gacggcacca gatagttgag gaatacgccg
     4021 acgacataca caaccagtgt cgctagaatc ccggcatacg gcacatgctg acgactcatt
     4081 ttcgccataa aactcggtgc ggaaccgccc atcgccattg agcgcagaat acgtccggtg
     4141 cagtacagac ctgagttcag gctggagagg gcagcggtca gcaccacaat gttcataatg
     4201 ctgccgatat atggcacacc cagtttagag aaaaacgtca cgaacggact ttgccccgcc
     4261 tgatacgcgc tccacggcaa taacataacc agcaacacca cggagccgac gtaaaacagg
     4321 ccaatacgcc aaatcacact gttaatggct ttaggcacca tggtctgcgg atctttacat
     4381 tctccggcag ctgtacccac catttcaatg gaggcaaaag caaacactac gccctgaatc
     4441 aacaccagcg caggcagcaa accgtggggg aagaagccgc cattatcggt gattaaatga
     4501 aagccagtgg tgttgccatc cagcggctga ccactaccga ggaacactgt acccacgacc
     4561 aaaaaggtca caatggcgag cactttaata agcgcaaacc agaactccat ctccgcaaac
     4621 catttcacac cgatcatatt catggtgcca acgatggtaa gtgcagcgag cgcaaagacc
     4681 cactgcggca cgccgccaaa cgcaccccag taatgcatat acagagcgac ggcggtaata
     4741 tcaacaatcc ccgtcatcgc ccagttgatg aagtacatcc agccagcaac ataagcggct
     4801 ttctcaccca aaaactcacg ggcataagag acaaaactgc cactggaagg gcggtgtagc
     4861 accagctcac ccaatgcacg cagaataaaa aacgaaaaca agccacaaat taaataaacc
     4921 agtgccagtg ctggacccgc catttgcagt cgggctcctg cacctaaaaa caagccggtg
     4981 ccaatcgcgc cgccaatggc gatcatctgt acctggcgat tgcccatcgc tttgtgatac
     5041 ccctcttcgt gggcattaag ccagcggcgt ttcgcggcgt gttgatctga agtgtcggtg
     5101 tcgtgtttac tcattgctct ccctgattgc tttaatgaaa aagtcatata agttgccatg
     5161 aacaatgttt attcatcctg gcgatgatag ttatcaccat tattctttat gggtataaaa
     5221 ctatcggcgc agcatcctac ccgttatcga tatacgatgc aaaacatccc cttacaatcc
     5281 tgaaggggat tcatacaact gacgaaaaaa tgacaaatac ttttgctggt taacctgtgt
     5341 actgtcctac acttaatctt taaaagattg tgaggggcat atgattaagg tgtatggcgt
     5401 acccggatgg ggctctacaa tcagtgagct gatgctaaca ctggctgata ttccttatca
     5461 gttcgttgat gtcagtggtt ttgaccacga gggaacttca cgcgagttat tgaaaaccct
     5521 gaacccgttg tgtcagattc cgacactgac gctggaaaat gacgagatta tgacggagac
     5581 agcggcgatt gcattgatgg ttctcgatcg tcgcccggac cttgcgccac cggttgggcg
     5641 cgccgaacgt cagcagtttc aacggctatt ggtctggctg gttgctaatg tctatccaac
     5701 attcactttc gccgattacc ctgagcgttg ggct
//
LOCUS       DAJTSL010000209         5581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTSL010000209 DAJTSL010000000
VERSION     DAJTSL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.211"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(25..762)
                     /gene="bamD"
                     /locus_tag="OO433_003784"
     CDS             complement(25..762)
                     /gene="bamD"
                     /locus_tag="OO433_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6052089.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            897..1877
                     /gene="rluD"
                     /locus_tag="OO433_003785"
     CDS             897..1877
                     /gene="rluD"
                     /locus_tag="OO433_003785"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6052090.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNLDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1874..2605
                     /gene="pgeF"
                     /locus_tag="OO433_003786"
     CDS             1874..2605
                     /gene="pgeF"
                     /locus_tag="OO433_003786"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6052091.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     EHVEENRKQLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2735..5308
                     /gene="clpB"
                     /locus_tag="OO433_003787"
     CDS             2735..5308
                     /gene="clpB"
                     /locus_tag="OO433_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6052092.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1389 a         1334 c         1512 g         1346 t
ORIGIN      
        1 gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga tgattttcgc
       61 tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca tcagtggcag
      121 cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta cgcggttaac
      181 gacggcaacc catgcaccac gttctgtata gtactcggca acggagtatt catatttcgc
      241 cagacgatct ttcaggaata ccagacgttt ggttgcatcg gtagtgtact gactgttcgg
      301 atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt gaggatcgcg
      361 atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg ccatattggt
      421 caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa ggcgaataaa
      481 acgatcgatg gcagcctgtg ctaacggcaa atcggcgttt ttatagtagg cgtagatgag
      541 atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta atgcttccag
      601 ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag tcgcgtaaat
      661 ttcatttggt ggattatcag gtacttcttc ctttgacccc gagcaacccg ccaaaaacag
      721 gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt gactttcctc
      781 aaaatgtaat acgggagatt ctctgttcct gctcccggtt aagaccagct acaatagcac
      841 actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca gtatatatgg
      901 cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa cgcttagatc
      961 aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa tggatcctcg
     1021 accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa gtattgggtg
     1081 gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa ccgcaggata
     1141 tcccgctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa ccgcgcgacc
     1201 tggtggtaca tcctggcgcg ggtaacctgg atggcacggt actgaatgcg ttgcttcatt
     1261 attatccgcc cattgccgat gtaccgcgtg cgggcatcgt ccatcgtctg gataaagaca
     1321 ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta gtcgaatctt
     1381 tgcaaaggcg tgaaattact cgtgagtatg aagcggtggc aattggtcat atgaccgctg
     1441 gcggtacagt agatgaacca atcagccgcc acccgaccaa acgtactcac atggcggtgc
     1501 atcccatggg caaaccggcg gtcactcact atcgcatcat ggaacacttc cgtgtgcaca
     1561 cgcgtctgcg gttgcgtctg gaaaccggac gtacgcacca gatccgcgtg catatggcgc
     1621 atattaccca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt ccgccaaaag
     1681 gtgcttcgga agcgtttatc tccacgctgc gtaagtttga ccgccaggcg cttcatgcaa
     1741 ctatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat gcgcctattc
     1801 cagaagatat ggtggagttg attgaggtta tgcgcgccga tttcgaagag cataaggatg
     1861 aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa aaggtgttgc
     1921 ggcctgtagc tctacccgta tcggcggcgt gagcttgcca ccttacgact ctctcaacct
     1981 tggcgctcat tgtggtgata acccggagca cgtagaggag aatcgcaagc aactttttgc
     2041 tgcgggaaat ttgccttcaa aaccggtctg gcttgagcag gtacacggca aagatgtgct
     2101 taagctcact ggcgaacctt atgcctcaaa acgggcggat gcctcttata gcaatacgcc
     2161 cggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt gcaatcgagc
     2221 gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag gcgtgctgga
     2281 agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt tagggccggc
     2341 aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta tggcagtaga
     2401 cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg atatttatca
     2461 gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg gcgaccgttg
     2521 tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca ccggtcgtat
     2581 ggcaagtttc atttggctga tataacctaa agaatcaaga cgattcggta cgcgtgattt
     2641 tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct catttaatct
     2701 ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc ttactaataa
     2761 attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca accaattcat
     2821 cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg ttagtccttt
     2881 attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc aggcattaaa
     2941 tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg atctggtgcg
     3001 cgttcttaat ctttgcgaca agctggcgca aaaacgaggt gataacttta tctcgtcaga
     3061 actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc tgaaagcagc
     3121 aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag gtgaaagcgt
     3181 gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca tcgaccttac
     3241 cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag aaattcgccg
     3301 taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg gtgaacccgg
     3361 cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg gcgaagtgcc
     3421 ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg tggctggggc
     3481 gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc ttgccaaaca
     3541 ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg cgggtaaagc
     3601 cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg gtgaattgca
     3661 ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag atgctgcgct
     3721 ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata ccattgcgat
     3781 tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta ctgacccggc
     3841 aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc tgccggataa
     3901 agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg actcaaaacc
     3961 agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac aacaggcgtt
     4021 aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg aagaactgag
     4081 cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaaa aggcatcgct
     4141 ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta ttgaacaggc
     4201 tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa tcccggaact
     4261 ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc tgttgcgtaa
     4321 taaagtgacc gacgccgaaa ttgctgaagt gctggcgcgt tggacgggga ttccggtttc
     4381 tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac tgcaccatcg
     4441 cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc gtagccgtgc
     4501 ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc caacgggtgt
     4561 ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg acgaggcgat
     4621 ggtccgtatc gatatgtccg agtttatgga gaaacactcg gtgtctcgtc tggttggtgc
     4681 gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg tgcgtcgtcg
     4741 tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg tcttcaacat
     4801 tctgttgcag gtactggatg atggtcgtct gactgacggg caggggagaa cggtcgactt
     4861 ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatttgattc aggaacgctt
     4921 cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtaa gccataactt
     4981 ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc tgggtgaaca
     5041 gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc tggaagaacg
     5101 tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga acggttacga
     5161 tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg aaaacccgct
     5221 ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc tggaagttaa
     5281 tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg ctcgtttttg
     5341 tctataagtt agacggaaaa gactatattt aagatgtttt gcctgaaaaa tgagcgaacg
     5401 ataaagtttt tatatttttc gcttgtcagg ccggaataac tccctataat gcgccaccac
     5461 tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga gaatctcaac
     5521 agagaaaagc aaaaaaatgc ttgactctgt agcgggaaag cgcattatgt acaccccgcg
     5581 c
//
LOCUS       DAJTSL010000210         5534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTSL010000210 DAJTSL010000000
VERSION     DAJTSL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.212"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            411..974
                     /gene="ahpC"
                     /locus_tag="OO433_003788"
     CDS             411..974
                     /gene="ahpC"
                     /locus_tag="OO433_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6052093.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1145..2710
                     /gene="ahpF"
                     /locus_tag="OO433_003789"
     CDS             1145..2710
                     /gene="ahpF"
                     /locus_tag="OO433_003789"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6052094.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2831..3259)
                     /gene="uspG"
                     /locus_tag="OO433_003790"
     CDS             complement(2831..3259)
                     /gene="uspG"
                     /locus_tag="OO433_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6052095.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLTQDDGVIHLLHVLPGSA
                     SLSLDRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            3480..4718
                     /locus_tag="OO433_003791"
     CDS             3480..4718
                     /locus_tag="OO433_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR6052096.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEEAQKAVSGLVNAMPGGTI"
     gene            complement(4949..5359)
                     /gene="rnk"
                     /locus_tag="OO433_003792"
     CDS             complement(4949..5359)
                     /gene="rnk"
                     /locus_tag="OO433_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6052097.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
BASE COUNT         1444 a         1319 c         1441 g         1330 t
ORIGIN      
        1 tgcaggaagc agagccagta aaagtatctt ttttaacatt aatttgtcct tttcagtcag
       61 tgcaaaagtc gagtaaaagg cataacctat cactgtcata ggtaagagct tagatcaggt
      121 gattgccctt tgtttatgag ggtgttgtaa tccatgtcgt tgttgcattt gtaagggcaa
      181 cgcctcagcc tgcaggcagg cgctgaagat accaaagggt agttcagatt acacggtcac
      241 ctggaaaggg ggccatttta ctttttatcg ccgctggcgg tgcaaagttc acaaagttgt
      301 cttacgaagg ttgtaaggta aaacttatcg atttgataat ggaaacgcat tagccgaatc
      361 ggcaaaaatt ggttacctta catctcatcg aaaacacgga ggaagtatag atgtccttga
      421 ttaacaccaa aattaaacct tttaaaaacc aggcattcaa aaacggcgaa ttcatcgaaa
      481 tcaccgaaaa agataccgaa ggccgctgga gcgtcttctt cttctacccg gctgacttta
      541 ctttcgtatg cccgaccgaa ctgggtgacg ttgctgacca ctacgaagaa ctgcagaaac
      601 tgggcgtaga cgtatacgca gtatctaccg atactcactt cacccacaaa gcatggcaca
      661 gcagctctga aaccatcgct aaaatcaaat atgcgatgat cggcgacccg actggcgccc
      721 tgacccgtaa cttcgacaac atgcgtgaag atgaaggtct ggctgaccgt gcgaccttcg
      781 ttgttgaccc gcagggtatc atccaggcaa tcgaagttac cgctgaaggc attggccgtg
      841 acgcgtctga cctgctgcgt aaaatcaaag cagcacagta cgtagcttct cacccaggtg
      901 aagtttgccc ggctaaatgg aaagaaggtg aagcaactct ggctccgtct ctggacctgg
      961 ttggtaaaat ctaaatttcc ttcgtctttc gcgctaccaa ctccggcgcg aaatacttcg
     1021 gaaattcacc taattcttcg ggtgctgcgg cacccgattt tcttccccgc actatgatgc
     1081 aagctgcatc caggtagccg tagaggccgc ttgcatgatg atgtttaaag cccaggagat
     1141 aaacatgctc gacacaaata tgaaaactca actcaaggct taccttgaga aattgaccaa
     1201 gcctgttgag ttaattgcca cgctggatga cagcgctaaa tcggcagaaa tcaaggaact
     1261 gttggctgaa atcgcagaac tgtcagacaa agtcaccttt aaagaagata acagcttgcc
     1321 ggtgcgtaag ccgtctttcc tgatcaccaa cccaggttcc aaccaggggc cacgttttgc
     1381 aggctccccg ctgggccacg agttcacctc gctggtactg gcgttgctgt ggaccggtgg
     1441 tcatccgtcg aaagaagcgc agtctctgct ggagcagatt cgccatattg acggtgattt
     1501 tgaattcgaa acctattact cgctctcttg ccataactgc ccggacgtgg tgcaggcgct
     1561 gaacctgatg agcgtactga acccgcgcat caagcacact gcaattgacg gcggcacctt
     1621 ccagaacgaa atcaccgatc gcaacgtgat gggcgttccg gcagtgttcg taaacgggaa
     1681 agagtttggt cagggccgca tgacgttgac tgaaatcgtt gccaaaattg atacgggcgc
     1741 ggaaaaacgt gcggcagaag agctgaacaa gcgtgatgct tatgacgtat taatcgtcgg
     1801 ttccggcccg gcgggtgcag cggcagcaat ttactccgca cgtaaaggca tccgtaccgg
     1861 tctgatgggc gaacgttttg gtggtcagat cctcgatacc gttgatatcg aaaactacat
     1921 ttctgtaccg aagactgaag ggcagaagct ggcaggcgca ctgaaagttc acgttgatga
     1981 atacgacgtt gatgtgatcg acagccagag cgccagcaaa ctgatcccag cagcagttga
     2041 aggtggtctg catcagattg aaacagcttc tggcgcggta ctgaaagcac gcagcattat
     2101 cgtggcgacc ggtgcaaaat ggcgcaacat gaacgttccg ggcgaagatc agtatcgcac
     2161 caaaggcgtg acctactgcc cgcactgcga cggcccgctg tttaaaggta aacgcgtagc
     2221 ggttatcggc ggcggtaact ccggcgtgga agcggcaatt gacctggcgg gtatcgttga
     2281 gcacgtaacg ctgctggaat ttgcgccaga aatgaaagcc gaccaggttc tgcaggacaa
     2341 actgcgcagc ctgaaaaacg tcgacattat tctgaatgcg caaaccacgg aagtgaaagg
     2401 cgacggcagc aaagtcgttg gtctggaata tcgagatcgt gtcagcggcg atattcacaa
     2461 catcgaactg gccggtattt tcgtgcagat tggtctgctg ccgaacacca actggctcga
     2521 aggcgcagtc gaacgtaacc gcatgggtga gattatcatt gatgcgaaat gcgaaaccaa
     2581 cgtcaaaggc gtattcgcag cgggtgactg tacgacggtt ccgtacaagc agatcatcat
     2641 cgccactggc gaaggtgcca aagcctctct gagtgctttt gactacctga ttcgcaccaa
     2701 aactgcataa gaagaagtaa gattcacctg caattgctta gccgccgggg tcaaacctag
     2761 cggctttttt atggcattaa aaagccctgc cgggatgttc caggcagggc ggcataaata
     2821 cttttatctg ttagcgaacg accagtacag gcagattggc gtggcggatc acgctcgagg
     2881 cgttagaacc taacagatgg gtcgaaatcg atgggttgcg agaaccgata actacaacat
     2941 cagcccccag ttcttctgcc aactcattga cttcatcccg cacgctacca aaacggacat
     3001 gttgtttaat gcgggaagga tcgatggtga agtggctgac catcgtttgc agacgttctt
     3061 ctgcttcatg ttgcagatgc tcttcaaaac gacgcacatc agcggcaaaa cggtccaggc
     3121 tcaggctggc tgacccgggt agtacgtgaa gtagatgaat aactccgtca tcctgggtga
     3181 ggaattcagc gtggcgaaca gctttgtcgc tcaattccat ttcaaataca tcaactggca
     3241 taatgattgt cttatacata accctttctc cctgttaatc atgaacaaat cattcgccat
     3301 gattataata tttatccctg atatttgtct ggttcttttt ccttacgaac ggtttctgtg
     3361 attaatatat tctcactgaa caccaggaat tctcccaaaa cctgcggtac cgcccgtttt
     3421 cccgctgtga tagctaccct taaagactga ctcttttttg aactgtctct ggaggttgca
     3481 tgaaagcatt gacttatcac ggcccacatc acgttcaggt agaaaatgtt cccgatccgg
     3541 gcattgaaca ggcagatgat attattctgc gtattacggc aacggcgatc tgtggctctg
     3601 atctccatct ttatcgaggc aaaatacccc aggttaaaca tggcgatatt tttggtcatg
     3661 aatttatggg ggaagtcgtt gaaaccggaa aggacgtaaa aaatttgcaa aaaggcgacc
     3721 gggtggtaat tccgtttgtc attgcttgtg gcgactgttt tttctgtcga atgcagcaat
     3781 atgccgcctg cgaaaatacc aatgcgggta aaggcgctgc gctcaataaa aaacagatac
     3841 cagctcccgc ggcattgttt ggttatagtc acctgtatgg tggcgttcct ggtggacagg
     3901 cggaatatgt ccgcgtccct aaagggaatg tggggccgtt taaagtaccg cctttgcttt
     3961 cagatgataa agcccttttc ctttctgata ttctgccaac ggcatggcag gcagcaaaaa
     4021 atgcgcagat ccaacaaggt tcaagcgttg cagtctatgg tgccggtcca gtgggattgt
     4081 tgacaatcgc ctgtgcacgg ttgctcggtg cggaacagat ttttgttgtt gatcatcatc
     4141 cctaccgctt gagtttcgcc gctgaccgct acggcgcgat cccgattaat ttcgatgaag
     4201 atagcgatcc ggcacagtca attattgaac aaacggcagg tcaccggggc gtggatgcag
     4261 taatagacgc cgtcggtttt gaagcgaaag gcagcaccac ggaaacggtg ctgactaacc
     4321 tgaaactgga gggcagcagc ggtaaagcgt tgcgccagtg tattgcggcg gtcaggcgtg
     4381 gcggcattgt tagcgtaccg ggcgtctacg ctggatttat tcacggtttc ctgtttggtg
     4441 acgcctttga taaagggttg acgtttaaaa tgggacagac ccacgttcac gcatggctgg
     4501 gagaattatt accgttaatt gagaaaggat tactgaaacc agaagaaatt gttacccact
     4561 atatgccgtt tgaagaggcc gcccggggat atgagatttt cgaaaaacgt gaagaggagt
     4621 gccgtaaggt gattctggta cccggtgcac aaagcgcaga ggaggcgcag aaggcagttt
     4681 caggtctggt gaatgcgatg ccggggggaa caatatgatc gtcaggagtg gttttcgagg
     4741 taaaggacag ccatgacgat aatcgccgcc ataatcagaa atcctatcag gatgtaaaat
     4801 gcttctgcca tggttattcc cacaaacgaa acggaataat tttgcagcaa aagtgaacag
     4861 tgagaaccag gaaaaattgc tgattttgcg taaagaggat gcgagtgcat cctctgggca
     4921 aagcgagtta tcgcttgtgc tgatgggatt aaagcaggta gtcgccagca gcttctggct
     4981 ggtattcgag ttccagcact tcaaggtggg ttgcaacgcc gcccggaagt tcccagtgaa
     5041 tggaatcgcc aacgcgcagc cccagcagtg cggcacctac cggagccata acggaaagct
     5101 gagtattgct atcggtcatt tttgccggat acaccagcgt gcgcacacgc acttcgccat
     5161 cgctaagatt gcggaattta acccggctgt tcattgtcac cacgtcgtgt ggcatctctt
     5221 ctggcgaaca catttgggcg cgatccaact ctgcgtttaa cgcgtcggcg attggcaaac
     5281 cagcataggc gggttgctcc agcagaatat cgatgcgttc ggcatccagg tcgttaatga
     5341 tgatagttgg tctggacatt tttactccat gtcgtcggta ctgcgagtgt cgcagataaa
     5401 catacccaaa agaaaaccct caccgtcagg cggcgagggt ttaactcaca tgatgatact
     5461 gactgttgct cactctttga agtgatttgc gtcacattca gggaattcct caatgcagcg
     5521 cattatgtat aatt
//
LOCUS       DAJTSL010000211         5467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTSL010000211 DAJTSL010000000
VERSION     DAJTSL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.213"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..930
                     /locus_tag="OO433_003793"
     CDS             <1..930
                     /locus_tag="OO433_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6052098.1"
                     /translation="TVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFPSTM
                     VDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVS
                     DVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAPTLK
                     VQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLL
                     ALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDD
                     LPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(966..1169)
                     /gene="ydfZ"
                     /locus_tag="OO433_003794"
     CDS             complement(966..1169)
                     /gene="ydfZ"
                     /locus_tag="OO433_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6052099.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1347..2031)
                     /gene="ydfH"
                     /locus_tag="OO433_003795"
                     /pseudo
     CDS             complement(1347..2031)
                     /gene="ydfH"
                     /locus_tag="OO433_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
     gene            complement(2120..2866)
                     /gene="ydfG"
                     /locus_tag="OO433_003796"
     CDS             complement(2120..2866)
                     /gene="ydfG"
                     /locus_tag="OO433_003796"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6052100.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3003..5048
                     /gene="dcp"
                     /locus_tag="OO433_003797"
     CDS             3003..5048
                     /gene="dcp"
                     /locus_tag="OO433_003797"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6052101.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5093..>5467)
                     /locus_tag="OO433_003798"
     CDS             complement(5093..>5467)
                     /locus_tag="OO433_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6052102.1"
                     /translation="ALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEK
                     VAAEMATGAIERADADVSIAITGYGGLEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAG
                     DCETVLALAVRFAHVQLLQLLL"
BASE COUNT         1357 a         1315 c         1413 g         1382 t
ORIGIN      
        1 accgtcatgt catgtgacaa catgccagaa aacggtcatg ttatgcgtga cgttgtcact
       61 tcatacgcga aagctgttga tgtaaaactg gcacaatgga tcgaagataa cgtgactttc
      121 ccatcaacaa tggtggaccg tattgtgccc gcagtgacag aggatacgct ggcgaaaatc
      181 gaacaactta ccggtgtgcg cgatcctgcg ggcgttgcct gtgaaccttt ccgccagtgg
      241 gtaatagaag ataactttgt tgccggacgt ccagaatggg aaaaagcggg agccgaactg
      301 gttagtgatg tgctgcctta tgaagagatg aagttgcgca tgctcaacgg cagtcattca
      361 ttcctggcgt atttgggtta tcttgccgga tatcagcaca ttaatgactg catggaagat
      421 gaacattatc gttatgcggc gtatggcttg atgttgcagg aacaagcgcc gacgctgaaa
      481 gtgcagggcg ttgatttgca agattacgct aaccgattaa ttgaacgcta tagcaatccg
      541 gcgttacgtc atcgaacctg gcagattgcg atggatggta gccagaaatt gccacagcgg
      601 atgttggatt ctgttcgctg gcatctggcg catgacagca agttcgatct gctggcgctg
      661 ggcgtcgcgg gttggatgcg ttatgtcggt ggtgttgatg aacagggaaa tccgatagaa
      721 atcagtgacc cactgttacc tgttattcag aaggctgtac aaagtagtgc cgaagggaaa
      781 gcgcgcgtcc agtcattgct ggcgattaag gcaatctttg gtgatgattt gccagacaat
      841 agcttgttta ctgcaaaagt gacggaagcg tacttgtctt tattagcgca tggcgcgaaa
      901 gcgaccgtgg cgaaatattc cgtgaagtaa aaaaagcaat gccgccatcg gcggcattca
      961 cacacttagt tcatgccgag acgaatcagg tccagaggtg ccagtttctc ttcacaacct
     1021 tcaacaacta cggtttttcc gcggcggatt tgctcagcgg tcagaccttc agtatcaatc
     1081 gacaggatct tgccagtgtg accggtgccg ctaaccataa cacggctgcc agtggtgatt
     1141 gcgttacggt tacgatcgta ggtcgtcata ggtgttttct cctttctgat ttacagtcga
     1201 agcccacgta acgggcgcaa tataaatact cctgtcgagt gaaatatttt ttgtttttga
     1261 tcaagctcac atcttttttg atgcatgcat gagccgtaag gcggggggaa aagcctctca
     1321 tcccggatgg gatgagagta aatgaattac tcttcgctaa accagtcgct gttttcctgg
     1381 cggatctggc cacggattcg ctgatttcct gcaaatgttg cgtcattgca cgttctaccg
     1441 catcgccatc acgtttttgc agggcagaga aaatatcaag atgctggcgt aacagcattt
     1501 ctggtggaga aacatggtcg aaactcatat agcgcacgcg atcaacggtc gctttcaggt
     1561 tctcaatggt atcccacgcc agttgacagt cggcaatctg cgtcaggagt tgatggaagt
     1621 tgtcatcaag ttcaaaaaat catccagttg cttgtgctca atggcaatgc gttgctggtg
     1681 aagattttgt tccagttggt agcactggct ttcggtaatc atgctcgccg cccgacgcgc
     1741 caccgcgcac tcaatggcct gacggataaa actgccgttg cgcacctggg ccatggaaat
     1801 tttgttgacg tagctgccac gctgtggacg aatttgaatc aggccgtttt ccgccagttt
     1861 aataaaggct tcacgaaccg gctggcgtga cacattgaaa cgaacagaaa cttctttttc
     1921 cgacaacggt gtgcctggag caatcaggca atggacaatg tcgcgacgaa ggatacgata
     1981 aatttgctga ttgacaggct gtgtgggatt aagttgcgtt tcgacggtca ttcgttcttg
     2041 cttgtgagtg agttaactgc atgagtctac cacttttttg tgacaagcaa taacccggca
     2101 gttacgccgg gtataaaaat tactgacggt ggacattcag tccggcatag ctttgggtaa
     2161 ccggcatcat ttccagggta ttgatattga cgtgagcagg cagcgttgac acccaccaaa
     2221 cggcttcgct gacatcttct ggcgtcaatg caacggtatt ttgataggtt ttttccgctt
     2281 taccgtcatc gcctttaaag cggacattgg aaaactcggt gccacccacc agacccggtt
     2341 cgatgtcggt gacgcgcacc gccgtaccat gcaggtccgt acgcagattc aggctaaact
     2401 gacgaacaaa cgctttcgtc gcaccgtaaa cgttaccacc ggcataaggc cagctacctg
     2461 ccgttgagcc aatgttaata atatgaccat gattacgttc aaccataccc ggtaagacgg
     2521 cgcgcgtcat atataccagg cctttgttgt tggtatcaat catcgtttcc cagtcttcaa
     2581 cgctggcttt atgcgcaggc tccatgccca acgccaggcc ggcattattt accaggatat
     2641 caatattgca ccactcggca ggaagcgatg ccagcatctc ttcaatagcg gcgcggttgc
     2701 gaacgtccag ttgggcgatg tacagattat ctcccagttc gtcttttaac tcctgcaacc
     2761 gttcctggcg acggccagtg gcgataactt tatgcccttg ttgaataaaa cgacgagtaa
     2821 tgcattcacc aaaacctgcc gttgctccag ttactaaaac gatcatctca ctgttcctca
     2881 acgcttttgt gtgtcaataa catagcacgt gatgcttaag agcgggtaat actggtttgt
     2941 aagattgcac tccgttatca gtaagcctac gctatggaac acctcgcgtt caggagaaga
     3001 aaatgacaac aatgaatcct ttccttgtgc aaagcacact accgtatctg gctccccatt
     3061 ttgatcaaat tgccaatcat cactatcgcc cggcattcga tgagggaatg cagcaaaagc
     3121 gggcagaaat tgctgccatc gcgcttaacc cgcaaacgcc tgatttcaac aatactattc
     3181 tggcactgga acaaagcgga gaattactta cccgcgttac cagcgtcttt tttgcgatga
     3241 ctgcggcgca taccaatgat gaattacagc gtcttgatga acagttttcc gctgaactgg
     3301 cggaactggc taatgatatc tatctgaacg gtgaattatt cgcgcgggta gatgctgtct
     3361 ggcagcgccg tgaatccctg gggcttgata gtgaatccat ccgcctggtg gaggtgattc
     3421 atcaacgttt tgtccttgcc ggagccaaac ttgcgcaagc tgataaagca aaattaaaag
     3481 tactgaatac agaagctgcg accctgacca gccagtttaa ccagcgatta ctggcagcaa
     3541 ataaatccgg cggtctggtt gtgaacgata tcgcgcagct ggcaggaatg agtgagcaag
     3601 agattgcgct ggcggcagag gcggctcgcg agaaaggtct ggataacaaa tggctgattc
     3661 cgctgctgaa taccacccaa caaccggcgc ttgccgagat gcgcgatcgt gcgacgcgtg
     3721 aaaaactgtt tattgcgggc tggacgcgag cggaaaaaaa tgatgccaat gatacccgcg
     3781 ctatcattca acgtctggta gagattcgcg cacagcaggc gaaactgctt ggttttcctc
     3841 attatgccgc atggaaaatc gccgatcaga tggcaaaaac gccagaagca gcactcaact
     3901 ttatgcggga aattgttcca gcggcgcgtc aacgtgctag cgatgaatta gcctccatac
     3961 aggcggttat cgataagcag caaggcgggt ttagcgcgca gccgtgggac tgggcatttt
     4021 atgccgaaca ggtacggcgg gagaaatttg atcttgatga ggcgcagctc aagccatatt
     4081 ttgaattaaa cacggtgttg aatgaaggtg tattctggac cgcgaatcag ctcttcggta
     4141 ttaagtttgt cgaacgtttt gatattcctg tctaccatcc tgacgttcgt gtgtgggaaa
     4201 tttttgatca taatggcgtg ggactggcgt tattttacgg tgatttcttc gcccgtgatt
     4261 caaaaagcgg cggtgcatgg atgggcaatt ttgttgagca atcaacgctt aatgaaacgc
     4321 atccggtaat ttataacgtt tgcaattatc agaaacccgc tgccggtgag cctgcgttgt
     4381 tactctggga tgatgtcata accttattcc atgaatttgg tcatacgctg cacggccttt
     4441 ttgcccgcca gcgttatgcc acgctttccg gcaccaacac gccgcgtgat tttgtcgaat
     4501 ttccgtcgca aatcaacgaa cactgggcaa cgcatccgca ggtattcgct cgctacgccc
     4561 ggcattatca gagcggggca gcaatgcctg acgaactgca acagaaaatg cgtaatgcca
     4621 gcctgttcaa caaagggtat gagatgagcg aactgcttag cgccgcactt ctcgatatgc
     4681 gctggcattg cctggaagaa aacgaagcaa tgcaggatgt cgatgatttt gaattgcggg
     4741 cgctggtggc ggaaaatatg gatcttcctg ctataccgcc acgctatcgc agcagttatt
     4801 tcgcccatat ttttggtggc ggatatgctg caggttatta cgcttatctg tggacgcaaa
     4861 tgttggccga tgatggttac cagtggtttg ttgagcaggg cggattaacg cgtgaaaatg
     4921 ggcagcgttt tcgcgaggcg atcctttcca gaggtaacag cgaagatctg gaacgcctgt
     4981 atcgacaatg gcgcggtaag gctcctcaga ttatgccgat gctgcaacat cgtggcttga
     5041 atatataaac accaccaatg gctggcctgg agatatcgcc aggccagcct ggttatagca
     5101 gtaattgcag cagctggacg tgggcaaacc tcaccgctaa agctaatacc gtttcgcagt
     5161 cgccagcaaa atgcataacc gcagtgtagg tctggccttt aatatgccac gcgaaccaga
     5221 cggtacccgc tggcgtacca tcttcaccgc cctccagtcc gccgtagccg gtaatggcaa
     5281 tactgacatc agcatccgca cgctctatgg cacctgttgc catttctgct gccactttct
     5341 cactcaccgc agaatatcgt tcaagagatt gctggcttac gctgaggatt ttcatctttg
     5401 cctgatcggt gaaagtaaca aagcctgcac cgtaaaattt gggtgtatct tcagctgcac
     5461 acagggc
//
LOCUS       DAJTSL010000212         5428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTSL010000212 DAJTSL010000000
VERSION     DAJTSL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.214"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..915
                     /gene="adeQ"
                     /locus_tag="OO433_003799"
                     /gene_synonym="yicO"
     CDS             <1..915
                     /gene="adeQ"
                     /locus_tag="OO433_003799"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6052103.1"
                     /translation="IPLSLRIGITSGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSH
                     GVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIG
                     EVDLSGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDS
                     VSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYA
                     TAGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMK
                     VCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            968..1420
                     /locus_tag="OO433_003800"
     CDS             968..1420
                     /locus_tag="OO433_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6052104.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1631..2821
                     /gene="nepI"
                     /locus_tag="OO433_003801"
     CDS             1631..2821
                     /gene="nepI"
                     /locus_tag="OO433_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6052105.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRCYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(2862..3155)
                     /locus_tag="OO433_003802"
     CDS             complement(2862..3155)
                     /locus_tag="OO433_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052106.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3377..4195
                     /gene="nlpA"
                     /locus_tag="OO433_003803"
     CDS             3377..4195
                     /gene="nlpA"
                     /locus_tag="OO433_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR6052107.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLHTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4222..5121)
                     /locus_tag="OO433_003804"
     CDS             complement(4222..5121)
                     /locus_tag="OO433_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6052108.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSLSPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRPEK"
     gene            complement(5232..>5428)
                     /locus_tag="OO433_003805"
     CDS             complement(5232..>5428)
                     /locus_tag="OO433_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6052109.1"
                     /translation="FQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALF
                     WLAIGMLGIAMICLLFIKDI"
BASE COUNT         1295 a         1235 c         1390 g         1508 t
ORIGIN      
        1 attcccttaa gtttacgtat tggtatcacc agcggaattg gattatttat cgccttaatg
       61 ggattaaaaa atactggcgt tattgtcgcc aataaagaca cgctggtgat gattggcgat
      121 ttaagttctc acggcgtgtt gttaggtatt ttagggtttt ttattataac cgtgttgtca
      181 tcacgtcatt ttcatgccgc ggtgctggtt tctatagtgg tgacgtcttg ctgtggatta
      241 tttttcggtg atgttcattt tagcggcgtc tattccattc cgcctgatat tagcggcgtc
      301 attggtgaag tagatttgag cggcgcgtta acacttgaac tcgccggtat cattttctcc
      361 tttatgctga tcaacctatt tgattcatca ggaacattaa ttggtgtaac tgataaagcg
      421 ggcttaatag atggtaacgg taaattcccc aatatgaata aggcgctgta tgttgatagc
      481 gtcagttcgg tggcgggtgc gtttatcggc acctcgtctg ttactgccta tattgaaagt
      541 acttctggtg tggcagtcgg tggccgcacg gggctgactg cggttgtggt tggcgttatg
      601 ttcctgttgg ttatgttctt ctcaccgctg gtggcgatgg ttcctcctta cgcaaccgcc
      661 ggagcgttaa tctttgttgg cgtgctgatg acttcgagcc tggcgcgcgt taactgggat
      721 gattttaccg aatcggtgcc tgcgtttatt accacggtga tgatgccctt tactttctcg
      781 atcaccgaag ggattgcact cggctttatg tcgtactgca tcatgaaagt gtgcaccggg
      841 cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag ctctgtttgc actgaagatt
      901 attctggtgg attagtaact ggcgagggtt tccagatatc atgagttctg attacgcagg
      961 agaactcatg atctggataa tgctcgccac gctggcggta gtgtttgtgg ttggttttcg
     1021 ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc agtgatcggc tgaatatcga
     1081 tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag tcagccggtg acgaattttt
     1141 acgttatttg catcgcccgg atgagtcgca cctgcaaaac gccgcgcagg tgttgctcat
     1201 ctggcaaatt gtcattgtcg atggtagcga acagaacctg ctgcaatggc atcggatttt
     1261 acaaaaagcc cgtcttgccg cgccgattac cgatgctcaa gtcaggctgg cgctgggttt
     1321 tctgcgcgaa accgaacctg aaatgcagga tattaatgct tttcagatgc gctataacgc
     1381 gttctttcag cctgccgagg gcgttcactg gctgcattaa aaccgcttcg tcttatcagc
     1441 ctacagaccg cgcctgacct gtcgggtgga taagacgttc acgccgcatc cggtaatctt
     1501 tgccctgttc cgtttcgatc ttaactccag ctcaagcaac accgtaaaag ttgcaaaaac
     1561 gttaaatgca tcacacattt caatgttaaa gtggtcggct tttcccctga aacatgccac
     1621 gggtaacacc atgagtgaat ttattgccga aaaccgcggc gcggatgcca tcacccgacc
     1681 gaactggtca gccgttttct cggtggcgtt ttgtgtcgcc tgtctgatta tcgttgagtt
     1741 tttgcccgtc agtttgttga cgccaatggc ccaggattta ggcatttcgg aaggggttgc
     1801 cgggcaatcg gtgaccgtga ccgcctttgt ggcaatgttt gccagtttgt ttattaccca
     1861 gacaattcag gctactgacc gctgctacgt tgttattttg tttgccgttt tgctgacgct
     1921 ctcctgcttg ctggtttcct ttgctaactc attcagtttg cttttaatcg gtcgtgcctg
     1981 tctggggctg gcgctgggcg ggttctgggc gatgtcggcg tcgctgacca tgcgtctggt
     2041 gccgccgcgt acggtgccga aggcgctgtc ggtgatcttc ggcgcggttt ctattgcgct
     2101 ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt atcggttggc gcaatgtctt
     2161 taatgcggcg gcggtgatgg gcgtgctgtg tattttctgg attatcaaat cattgccttc
     2221 actgccaggc gaaccctcgc atcagaaaca aaatactttc cgcttattac aacgtccggg
     2281 tgtgatggca gggatgatcg ccatcttcat gtctttcgcc gggcagtttg ctttcttcac
     2341 gtatattcgc ccggtgtata tgaacctggc gggattcggc gtggatggct taacgctggt
     2401 gctgttgagt tttggtatcg ccagctttat tggtacgtcg ctttcgtcgt tcattcttaa
     2461 acgttcggta aaactggcct tagcaggcgc gccgttaata ctggctgtga gtgcgttggt
     2521 actgacgttg tggggaagcg ataaaatcgt tgctaccggc gtggcgatta tctgggggct
     2581 aacttttgca ttggttcccg tcggctggtc aacgtggatc acccgctcgc tggccgatca
     2641 ggcagaaaaa gccgggtcta ttcaggtggc ggttattcag cttgctaata cctgtggcgc
     2701 ggcaatcggc ggttatgcgc tggataatat tggtctgact tcgccgttga tgttgtccgg
     2761 cacattgatg ttgctgactg cattgttggt tactgcaaag gtgaaaatga agaaatcctg
     2821 atatcaggta aagaagcgtt ggtcagtagt tgttgctgtt cttacatatc cgggcattct
     2881 accttaccca acgcttccca ataattgctc tgattattac gttccatcgc cacgatggcc
     2941 tcgcgtatat gttgtttatt gttttcatct gacaacatta acttttccca cgcttcatct
     3001 gataatgacg cctgtggcgt gcaaatttta cgtaaatctt gtaataccgt ggttttctct
     3061 tttgcggact gcagtgagct aaaaggcgac tcggcgtata cgatgcctgg catagcgaaa
     3121 aaagtgaaaa taagcagtag tgtcgttggt ttcatggatt caacctttta caaatgtgta
     3181 tcgaatgaag cataagtgta gtttgctttc atcgctctca tcattctgta cgaaacgcgc
     3241 ttgcgtggtc agtaagaagt gccagacttt atattccact tttattcctt tttattctta
     3301 tcgatagcgt ttcgtttttt aaaccgcagc gaccttaccg ctatagtcag gtaatcatta
     3361 ataaaaggat aaaaaaatga aactgacaac acatcatcta cggacagggg ccgcattatt
     3421 gctggccgga attctgctgg caggttgcga ccagagtagc agcgatgcaa aacacattaa
     3481 agttggcgtt ataaatggcg cagaacaaga tgtcgcggaa gtcgctaaaa aggtggcaaa
     3541 agagaaatat ggcctcgatg ttgaactggt aggatttagc ggttcgttgc taccgaacga
     3601 tgcgacaaat catggcgaac ttgatgcgaa tgtcttccag catcgaccct ttcttgaaca
     3661 agataatcag gcgcatggtt ataaactggt agcggtggga aatacgtttg tcttccctat
     3721 ggcgggttat tccaaaaaaa tcacaaccgt ggcgcaaata aaagaaggcg cgacagtggc
     3781 gatccccaac gacccgacca accttggtcg cgcactttta ctgttgcaaa aagagaagct
     3841 aattactctg aaagagggaa aagggttatt acataccgcg ctggatataa ccgataatcc
     3901 tcgtcatttg cagattatgg aactcgaggg ggcgcagctt ccgcgcgtac tggatgatcc
     3961 caaagttgat gtagcgatta tcagcaccac ttacattcag cagacagggc tttctccggt
     4021 gcacgacagc gtatttattg aagataagaa ttcgccgtat gtgaatattt tggtggcacg
     4081 ggaagataat aagaatgcag aaaacgtgaa ggaatttctg caatcttatc aatcacccga
     4141 agtcgctaaa gcggcagaaa ccatttttaa cggtggcgcg gtgcctggct ggtaattctc
     4201 actcatgccg cgccggacga tttatttttc tggccggcga cgggaatcca ttgaaatcaa
     4261 aatcaccgat gacagaatca gcagcgttcc cagccagtcc ggtaatgtga acgtgatccc
     4321 cagcaacagc aaagagagta gcgcgctact taatggttct gcacagctca aaatgctggc
     4381 ttttggaccg ccaattaatt gtgctccttt caggtacaga ctaaatgtca gggacgtacc
     4441 aatgaccacc aaataaaaaa acgccagaat caaactgccg ttaacgacaa agtttgttcc
     4501 ttgtctggca taaaaaggca acagaatcag accgccaatc agcatactcc agccgacgac
     4561 tggtaacgtg ccatagcggg caattagcgt tgagggatag gtggtataga atgcagcagc
     4621 aaatgccgag gcaatgcccc agaacaacgc ggcaggagag agcgataatg acgtcggatt
     4681 accgtgtgtc accaataaaa aagtcccgac cagcgatgtc aaaatagcgc agaaaaccag
     4741 aatgcccggg cgcgatttac gcaccagtga gaaccaggcg acgataatcg tcggtgagag
     4801 gaattgcagc accgttgccg tggctgcgtt cgatttttcg atggttagca aaaaagtgag
     4861 ctgtacagtt agcgcgccaa ccacggaaaa aatcagcagg ctaatggcat ctttatgatt
     4921 gttaataata gaaaagattt tatcgccatg aacaaatgac agcgtcagta gaatcaaacc
     4981 ggcgaatatc aaacgcgtca tagtcaaaaa ctgcgacgac atctggcttt gctccatgat
     5041 gtattgcgcg cagacccctg aacttcccca caacacggcg gcaatcagaa cgttcagcat
     5101 cccctttctg gtggaaccca tttttccctc tgcatactgt tttgtcgttt atgtatgcag
     5161 gcatgatagc aaaaatggcg aggatggctc tgtttgcgcc acgccgattt ttcttagccc
     5221 agactggtga gctaaatatc tttaataaac agcaggcaaa tcatcgcaat acccaacatc
     5281 cctatcgcca gccagaacaa cgcatggtag ctccagattt caaccataat tccgtcaaca
     5341 gagccggcga taatccagcc gacgcgtgaa gtatttgcat ataacgttgt cgcagagcct
     5401 attttttcag gcattaagtc ctgaaaat
//
LOCUS       DAJTSL010000213         5361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTSL010000213 DAJTSL010000000
VERSION     DAJTSL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.215"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            30..1415
                     /locus_tag="OO433_003806"
     CDS             30..1415
                     /locus_tag="OO433_003806"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6052110.1"
                     /translation="MEGGWNEGGKGISIADVMTAGAHGVPREVTEGVIDGLNYPNHEA
                     IDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQ
                     GMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTCYKEKVKYWMTFNEIN
                     NQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFN
                     IGCMIAMYPIYPLTCVPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLD
                     ITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQ
                     IDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKK
                     AVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWY
                     RDLIANNGENI"
     gene            complement(1472..1690)
                     /gene="marB"
                     /locus_tag="OO433_003807"
     CDS             complement(1472..1690)
                     /gene="marB"
                     /locus_tag="OO433_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6052111.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1722..2105)
                     /gene="marA"
                     /locus_tag="OO433_003808"
     CDS             complement(1722..2105)
                     /gene="marA"
                     /locus_tag="OO433_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6052112.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(2125..2559)
                     /gene="marR"
                     /locus_tag="OO433_003809"
     CDS             complement(2125..2559)
                     /gene="marR"
                     /locus_tag="OO433_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6052113.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2771..3436
                     /gene="marC"
                     /locus_tag="OO433_003810"
     CDS             2771..3436
                     /gene="marC"
                     /locus_tag="OO433_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6052114.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(3461..4651)
                     /gene="ydeA"
                     /locus_tag="OO433_003811"
     CDS             complement(3461..4651)
                     /gene="ydeA"
                     /locus_tag="OO433_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6052115.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4801..>5361)
                     /gene="yneK"
                     /locus_tag="OO433_003812"
     CDS             complement(4801..>5361)
                     /gene="yneK"
                     /locus_tag="OO433_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6052116.1"
                     /translation="TAADYLRQFDLLVLNFISRGNFVILPRLWSNSEVHRWFVNKDPN
                     LITAILDITDGELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQESPHFQDFFLFF
                     ANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHEDL
                     VKNRPRSPVRPPIPATAKTPDLPERH"
BASE COUNT         1440 a         1203 c         1321 g         1397 t
ORIGIN      
        1 tatggggcgg cgcggtagcc gcgcatcagt tggaaggtgg ctggaatgaa ggaggaaaag
       61 gcatcagtat cgctgatgtg atgactgctg gcgctcacgg ggtgccgcgt gaagtgacag
      121 aaggcgttat cgacgggctt aattatccca atcatgaagc aattgatttt tatcatcgct
      181 ataaaacaga tattcagtta tttgccgaga tgggattcaa atgctttcga acttccattg
      241 cctggacacg aatctttccg caaggtgacg aacaggagcc gaatgaagag ggtttacaat
      301 tttatgatga tctgttcgat gaatgcctta agcagggaat ggaacctgtg gtgacgcttt
      361 cacattttga gatgccttat catctggtga caaaatatgg tggctggcga aaccgtaaac
      421 tgatcgactt ttttatccgc ttcgcatcaa cggtcttcac gtgctataaa gaaaaagtaa
      481 agtactggat gacgtttaac gaaatcaata atcaggtgaa tttcagcgaa agcctgtgtc
      541 catttactaa ttccggtatc ttgtattcgc cagaggaaga tatcaatgag cgcgaacaaa
      601 taatgtacca ggcggtacat tacgagttag ttgccagtgc cctggcggta cagactggaa
      661 aatcgatcaa tcctgaattt aacattggct gtatgatcgc catgtatccc atctatcctc
      721 tgacgtgtgt acccaacgat atgatgatgg ccacgaaagc aatgcatcgt cgttactggt
      781 ttactgatgt tcatgctcgt ggatattatc cgcaacatat gctgaattac tttgccagga
      841 aaggattcaa cctcgatatc acaccagaag ataacgcgat tcttgccagt ggttgtgtcg
      901 actttatcgg ctttagctac tacatgtctt ttacgacaca attttcgccc gataacccgc
      961 aactggatta tgttgaacca cgagatttgg tcagcaaccc ttatatcgat acatccgaat
     1021 ggggatggca aattgatccg gcagggctac gttattcact caactggttc tgggatcatt
     1081 tccagttgcc gctgtttatt gtcgaaaatg gatttggtgc ggttgaccag agacaagctg
     1141 acggcacggt gaacgatcac tatcgcattg attactttgc ttcccatatt cgggaaatga
     1201 aaaaagccgt agttgaagat ggtgttgact taattggcta caccccgtgg ggctgcattg
     1261 acctggtttc tgccggaaca ggggaaatga aaaagcgcta cggaatgatt tatgtcgata
     1321 aagacaacga agggaaggga acgctggaac ggatacgtaa agcgtcgttt tactggtatc
     1381 gggatctcat cgccaacaat ggcgaaaata tttgagactt gaggaagata ttacggagat
     1441 gtcaggctct gtgatcagag cctgacaata actacatagc gtgttgatta taatagggca
     1501 cgccgagcgc atccgactta tcactgccag tacccatgtg atttaaatca aacggtggtt
     1561 gttcctgcga tgggggaaca accacgacat tggcacaaga agtaacaact ggctgcgtgg
     1621 tttgttccgc aacgccctgc gcggaaaaga gaataagcgc agctgctatt gcggatgaaa
     1681 gtggtttcat gattgcctca gtgacgttgt cacgttatca actagctatt gtaatgattt
     1741 aatggatgta aaaagcgcga ttcgccctgc atattggtca tccggtattt atgtggcgga
     1801 acatcaaagt aatttttgaa ggttcgggtc agagtttgtt gcgactcgaa gccatatcgt
     1861 tctgccagat agagtatcgg ctcgttactt tccttcagct tttgcgcgat ttccgtcatc
     1921 ttacggctgc ggatgtattg gcctaatgaa tgaccggttt cttttttaaa catccgttgc
     1981 aggtgccatt tagagtaacc tgaacgctct gacactttct ccagtgacag tggcgattcc
     2041 aggttgtcct cgatccagtc caaaatgcta tgaatggtaa tagcgtcagt attgcgtctg
     2101 gacatcgtca tacctctttt ttgtttacgg caggactttc ttaagcaaat gctcaagtgt
     2161 tgccacttcg tccgccgtca ggttttttgt taattcttga tgcaggtcct ggccaactaa
     2221 ttgatggcat tgttcgcata ttgccgcgcc gctggtggta agttttacca gtacgccgcg
     2281 cttatcattc gggttcggca acctttcaac ccagccttta cagaccaggc gatccagcat
     2341 acgggtcagt gctccaaggt cgacagacaa cacttttttc agttcaaccg gagtaataca
     2401 cgccgcgcag cggatagagc agagcacctt aaactgtgcc gcggtaatat ccagcggaga
     2461 cagatactcg ttaagcagac gatctttctt ctgattaacc atatggatta agcgacccaa
     2521 tggaataatt tcattgaaca gatcgctggt acttttcaca ttagttgccc tggcaagtaa
     2581 ttagttgcag ggggtaatat tgcccaggca agtataagtc aaataaatga attgaccgat
     2641 gccacgtttt gctaaatcgg ttcaagtttt ggtcaatgcg atgcaaggtt agctaaagag
     2701 tgacacttta tccttgttta ccccttatac ttttcgctga taatccagat acactggata
     2761 acaaccacca atgttagatt tgtttaaagc aattggcctg gggctggtgg tgttactgcc
     2821 gttagctaac ccattaacaa ctgtcgcgtt atttcttggc ctggcaggca acatgaacag
     2881 tgccgaacgt aatcgtcagt cgttgatggc ctcggtgtac gtatttgcca tcatgatggt
     2941 ggcgtattac gccgggcaac tggtgatgga tacatttggc atttcgattc ccggtctgcg
     3001 aattgcaggc ggcttaatcg tcgcgtttat cggttttcgg atgctctttc cgcaacagaa
     3061 agcgattgat tcaccggagg cgaaaagcaa gtcggaagag ctggaagatg aacccagtgc
     3121 caatattgcc tttgtgccgc tagcaatgcc aagtaccgct ggtccgggaa ccatagcgat
     3181 gattattagc tcggcgtcca cagtgcgtca gagttcaacc ttcgctgatt gggtactgat
     3241 ggttgcgccg ccgctgatct ttttcctggt ggcggttatt ttgtggggaa gcctacgcag
     3301 ctccggcgca attatgcggc tggtgggcaa gggtggcatt gaagcgatct cccgtctgat
     3361 gggattcctg ctggtatgta tgggtgtgca gtttattatt aacggcatcc tggaaatcat
     3421 taaaacgtac cattaaaaaa ggcccgaatg ggcctttcaa ctattgcgtc tgttcttcga
     3481 gtgtcactgg ccagcggcga aatataatga ttgaccatat taacgcggca aaagcaggca
     3541 ccgcgcccac ataaccaatc atcgacattg accagtgcaa actcacctga ttacctacca
     3601 acgcacccgc cccgattcca atattaaata tgccggagaa tagcgccatc gcgacgtcgg
     3661 tagcatctgg tgccagcgcc agcactttaa cctgcatacc aagcccgatg atcatcatcg
     3721 cgatccccca gaaaatactc agcaccccga ggtgtatttc actgttcgcc gcaggtagca
     3781 gcagtgccag acacaccagc aacagcgcaa tcgccgtact caccaacgca gacgcatact
     3841 gattacccag tttaccgaaa atcacgctgc caataatgcc cgcaccaccg agtaataaca
     3901 gtaatgccgt ggcaaagttg gcgctgaatc ccgcaatgtt ttgcacaaaa ggctcgatat
     3961 agctgtatgc cgtgtaatgg gcggtgacaa ccaccacagt taacaaataa atgctcatca
     4021 atgccgggcg gcggaacaat agcgggaggc ttttcaatga accggaatgc tcactgggca
     4081 gtaagggaag taacttaatc aggcacaaaa gggtgataag cgccccaata ccaatcgcga
     4141 agaaggtcat tcgccaaccg aaatactgcc ccacaatgcg cccgagaggt aaacctaaga
     4201 ccatcgccag tgctgtaccg gtggcaatta aactcaaggc ctgtgcccgc ttcccggccg
     4261 gagccatacg gatcgccaga gacgccgtaa tcgaccagaa aatcgcatgt gcaaaagcca
     4321 caccaatgcg actgatcacc agaacggtaa agctccacga taaaaacgac agtacgtggc
     4381 tggcaataaa caccacaaac aggcagatca gtaatttgcg ccgttcaacc tgactggtca
     4441 ttaacataaa aggcaatgac attagcgcta ctacccatgc gtaaatggtc aacatgatgc
     4501 cgacctgagc ggtttgcatg tgaaaacttt gcgcaatgtc agagagcagg ccaacaggga
     4561 caaattcggt ggtgttgaag atgaaggcgg cgactgccag cgtaacgacc cgtagccacg
     4621 ccactttgcg ggaaacagtg tttgttgtca taacagatta tcgggtgagt tgcgaaaaag
     4681 tgatgaaatg atcttaaaac ggttaatgat gtgaatacaa tgcatttgtg atctggatcg
     4741 cgttttccgt ttctctgacg ctgattctgt tccgcgtcag agagtcggta acatcacggt
     4801 ttaatgacgt tcagggaggt ctggcgtttt ggcggttgcg ggtataggtg gtctgacagg
     4861 ggagcgaggc ctgtttttca ccaggtcttc gtgagtaact tttggcggcg tttcattttt
     4921 tacaaacagt ttgagggtcc tgcgcgccat atgcttgata attttatcat cacttaatga
     4981 tgctggcatc aacacgtcat tcagatcact ttcattggga ttcataaatt ggtgatagtg
     5041 caataacata ttggcgaaaa ataaaaaaaa gtcctgaaaa tgaggagatt cttgttctgc
     5101 taaaagggat aaaaagcaga tgcagacttc gggtagtaca tgtttgttag aacctaatga
     5161 atccatcagg ctttgcaata aatcctcttt taattcgccg tctgttatgt cgagaatggc
     5221 ggtgatcaag ttaggatctt tattgacgaa ccatctgtga acctcagagt tactccataa
     5281 tcgggggagt atgacaaaat taccgcgaga gataaaattc aggacaagca aatcaaactg
     5341 gcgtaagtaa tctgcagcgg t
//
LOCUS       DAJTSL010000214         5338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTSL010000214 DAJTSL010000000
VERSION     DAJTSL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.216"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(891..1832)
                     /gene="fabY"
                     /locus_tag="OO433_003813"
                     /gene_synonym="yiiD"
     CDS             complement(891..1832)
                     /gene="fabY"
                     /locus_tag="OO433_003813"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6052117.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1877..2314)
                     /gene="dtd"
                     /locus_tag="OO433_003814"
     CDS             complement(1877..2314)
                     /gene="dtd"
                     /locus_tag="OO433_003814"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6052118.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2311..3184)
                     /locus_tag="OO433_003815"
                     /pseudo
     CDS             complement(2311..3184)
                     /locus_tag="OO433_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
     gene            complement(3178..3777)
                     /gene="yihX"
                     /locus_tag="OO433_003816"
     CDS             complement(3178..3777)
                     /gene="yihX"
                     /locus_tag="OO433_003816"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6052119.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3918..4805)
                     /locus_tag="OO433_003817"
     CDS             complement(3918..4805)
                     /locus_tag="OO433_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6052120.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4795..>5338)
                     /locus_tag="OO433_003818"
     CDS             complement(4795..>5338)
                     /locus_tag="OO433_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6052121.1"
                     /translation="GIVYISSQLVLSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQK
                     SLYLSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGT
                     KVRYTGLSFAYQFSAILGGLSPLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIE
                     PTDEQETASYRYIREQSHEN"
BASE COUNT         1442 a         1346 c         1252 g         1298 t
ORIGIN      
        1 ctgataaatg aagaaaaacc gtattcatcg ccattaccac caccttacct atattactca
       61 ttaatgtatg tgcgaaaatt acgccggaaa atcattaaaa aaaagcggat ttttgcatgt
      121 tgttttgttt ttgcgaggcg ttttccagaa ataataagag tgttgcagtt gtttcattta
      181 ttactcaaaa atggtttagt tatcgcccag gatgaattat gggtgctatg aatatattat
      241 attcagagat atttatttgc ataatctgaa tggtgtagcc gaaaaacgtc agcatcctaa
      301 cagcaccagg atttaaggtg aaattaatct ttcatcacag accggtcagt actgccacca
      361 accggtcttt acttgctcat cgttataatt ttgttggtaa cagttcggga aagttgtttt
      421 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      481 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      541 gtaaatgaat gatactgaca atgatgccag cccattatct ttgcataatg tttgtgagct
      601 atagatttta aaaatattta tgaggaaagt atggcaccag aggaggtttt gactatcaaa
      661 aatcgctcaa acctgaacta agatgatgag ttaagcatta tctgcgataa ttgccactca
      721 gctctacaaa taatatttaa atgctcctga acagtgatgt agcaagacac gtatggtgcc
      781 aacgaagaat aaaaatttat agagttctta ttcaatatta ccggcataga cagggaatga
      841 tgtaaatgac cggaatgaat gagagaggca gcaccgcccc tctccataag ctactcttct
      901 tcgttcccgc cctcttcata cgggccaaat ggcttcgcgg gcagaacgat atacgtgcct
      961 tcaaacactg cacccggcgt ctcgtcgcca aagatttcaa cttgcatctg cacccgtgct
     1021 tttcgtccgc gcgccagacg gtcgagatcg ccgcttaagg caccaagatc ggctaccgca
     1081 tgaggtttac cgctaatcgg cttgctgtag cggatatgcg catcggccag aataatcgtt
     1141 ccgccgaggt ggcgttcgcg cagcatcagc cagataagcc cccaaccggt gagcgttgcc
     1201 agcgagaata aactcccggc aaacagcgta tggtgcggat tctgattacc ggtttccggc
     1261 atggtggtga taaatttttg cccggtatat tgctgaatgc gcacgcccat tttttcacta
     1321 agcgggatgt gttcgtacca cgcctgttgc agctgcgcgc accagtcgcc gcgatgcaaa
     1381 atatcatcca gagtggcaac aggtttaatc atcaaaaaat ggcgaatcgg cgtggtggtt
     1441 ggtgtggtga tttctccctg attaacaaac cccagcttgg cgaaaaactc caccgcgtct
     1501 tcacgggcgc tacaggtcac gcgcttaacg ccttcctgac gcgccaccga ctccagggtc
     1561 atcgccatca acgtccccag ccctttgtcc tgcacgtcgg gatgaacggc cataaagcga
     1621 atggacgctt cattgtcggc attaatatac agtcggccta ccgccaccag attcccctgc
     1681 tcgtcgacga ccatctgatg atgcgccatc gcatcccacg cgtcgcgttc cgaacctttt
     1741 ggttgatgca ggggcttacg caacatttcc cagcgaaact gatagtaacg ctctaactct
     1801 tcttctgttt gtggaacccg aaggtgatac atagcgatac tctctcttgt ttcccgtgac
     1861 aaccctggaa gctggctcat acctgcaacc agaatgtcac ggggccatca ttgaccagcg
     1921 atacctgcat atcggcagcg aagcgtcctg tttgcgtgtt catctcttgc tggcggcagc
     1981 gttcgacgaa atagtcatat aacgcctctg cgcgatccgg tgatgcgcct ttggagaaac
     2041 ttgggcgcat cccccgttcg gtatctgcgg cgagggtaaa ctgggaaacc accagtacac
     2101 tgccgcccgc ctgttgcacg ttgagattca tcttgccttc ggcatcgcta aagatgcggt
     2161 agccgagcac acgctcgcac agacggtttg ctttctgttc gtcgtcatcc ttttcgacac
     2221 ccaataacac caaaagtccc gcgccaattt cgcccgtcac ttctccctcc acggtgacgc
     2281 tggcacgggt tacgcgttga attaatgcaa tcatggttcg tcgtcttctt cttgttcagc
     2341 tgcttgtttt agtttgcggt attccccgag agtgacagta atttccgcgc caagcaagac
     2401 gatacaccat gtccagtaga cccaaacaaa gagaatgggg atcaccgcca gcacaccgta
     2461 aatgagctga tatgacggga acatggtgat ataaagcgcg aaacctttct ttcctgcttc
     2521 gaacaggagt gcggcgacaa acgcgccgac aatcgcgtcg cggttaggta cgcggatggt
     2581 aggaacaatg ctgtacagca accagaagga gatccacgac aacagcaacg gaaaaatacg
     2641 cagcacgttg tcgatgacag tattgagatc gctcgcccag cgcagggaga gcaaatagga
     2701 actgatcgcc agactggccc ctgccagcag cggccccagc gttaaaatca tccagtacac
     2761 ggcgaacgag taaatttttg gtcgcgctcg tttactgcgc cagatggtat tcaacgcgct
     2821 atcgatggag tacatcaaca ataacgcggt gacgatcagc ccgcacgccc caacggcggt
     2881 catcttgttg gaattggcaa caaattgttc gatataccgc tgaataacat cgccagtagc
     2941 aggcagaaag ttggcaaaaa taaagtgacg taactgaatg ctgacgtcgg aaaacatggg
     3001 aaaagcggcg aaaagcgcaa aaacaacggc aaccagcggc actaatgaga gcaacgacca
     3061 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     3121 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     3181 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     3241 aatgcccagc tgattggctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3301 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3361 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3421 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3481 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3541 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3601 ctctgcgaac gcttcgtcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3661 atgaaaactc ttcttaagcg tagccagtgg aacacgcgtt aaatcgctcc aggctcccag
     3721 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3781 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3841 gcaataaggg taatcttgtg atgctgattg cgaaaaagca gagtaatttc actggctttt
     3901 tcgcaataga tgaatggtta agattcaggt gtaaattgcc aacgagtttc gatgaccagc
     3961 ggcctatctg gtgtcaccaa ttccggcgtt agatttattg cgttaggtgc ctgagtcagc
     4021 gggttaatac aggtggcatc gggctgttta tcaaatacca ccagactgtt tgcagaagag
     4081 atcatggtca tcgcgagttt accaggccac agtaacttaa ccttcacccc gttataaaaa
     4141 ccaaaacagt catcccaggg accggcttga ggagagatac gatttccagt tggcagttca
     4201 tcactacccg cttcttcttg ccagtccgca tcaaaaagca cctgcaacga ttctgtattt
     4261 tgcggagtca gctttttcgc aaaccacgga tgccagccag cggaggccgg gaatgtatcg
     4321 gcatatgaat ggacttccag ttgcaaaacc agcgcatcgt tctccagcaa aaaagtctga
     4381 atcacttccc cttgccaggg ccacggtgaa gccaggggca tacgtaaagt cagggaatct
     4441 gctgttttgt cgatgatttc ccacgttgag taacaagcca taccatgcaa cgcatgtggt
     4501 ggtttattgg cgggtaaaga ataacattgc ccaccagcat tcagcgtcgc attccccagt
     4561 cgccctgccc acggcaccat tgggaaacag ccatactgaa aagctctgcg ctgcggctgc
     4621 cattggcgta atacttcaga accaaatgcc tttaacgagg ttatcctggc accatcatgc
     4681 ggataaatct gcaggtgaat attgcctttt tccagcgtca ctaactctcc tacaggccca
     4741 ggccatggcg cgtcaaacgc ctgttgtagc gccaggtaat gctcgttttt tcgttcagtt
     4801 ttcatgagat tgttccctga tgtagcggta agaagcggtt tcttgttcat cggtcggctc
     4861 aatcagccag acgcaaacaa aggaaagaac tgagacagca aataaaaaca gagcgacata
     4921 ccacggtttt cctcccccta gcgccagcaa acttgaggcg ataagggggc ttagtccgcc
     4981 aagaatcgcg gaaaattgat aagcaaaaga aaggccggta tatcgtactt tggtaccgaa
     5041 catgcgggtg aaaagtgttg gctgcacggc aaacatcatc gtcggaccta agttgtagcc
     5101 gaggatcata ctgcaccaga tgatgagcgt acttttgcta tccagtaata agaagaaagg
     5161 aaacgcgaac aggacacaaa atcctgctcc cgacaaatac agactttttt gacctatacg
     5221 atctgataac catccaacca atggacaact gaaaataccg atcgccgagg caatcaacat
     5281 acccgtcagg ggaatatctc gcgacaatac taattggctg gaaatataga caatacca
//
LOCUS       DAJTSL010000215         5334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTSL010000215 DAJTSL010000000
VERSION     DAJTSL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.217"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(117..998)
                     /locus_tag="OO433_003819"
     CDS             complement(117..998)
                     /locus_tag="OO433_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6052122.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTIRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            1099..2487
                     /gene="sad"
                     /locus_tag="OO433_003820"
     CDS             1099..2487
                     /gene="sad"
                     /locus_tag="OO433_003820"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCR6052123.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2551..3477
                     /gene="glsB"
                     /locus_tag="OO433_003821"
     CDS             2551..3477
                     /gene="glsB"
                     /locus_tag="OO433_003821"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6052124.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            3477..3836
                     /locus_tag="OO433_003822"
     CDS             3477..3836
                     /locus_tag="OO433_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6052125.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            3975..>5334
                     /locus_tag="OO433_003823"
     CDS             3975..>5334
                     /locus_tag="OO433_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6052126.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIVAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYR"
BASE COUNT         1245 a         1238 c         1463 g         1388 t
ORIGIN      
        1 aagaaatatg taattactga tgcttattgg cgtaaccata tttgccccat atagtcataa
       61 gtcatcggaa catcactata tagggcattt gagctgcaag aactatcaaa aatagctcat
      121 tggtaaccct gttttgccga gtcaggcaca tccagtagtt gaataaatgc ctcaagcgga
      181 cgcgttttcg caccacgacg ccagaccagc caggttgtta accaacgcca ttgctcagct
      241 aacggccacg cttcaacctg gtgatgcccc ggcatacttt ccagcataga gcgtggaata
      301 agcgcaatgc ctgctcctgc gaccacacag gccaacatcc cgtgatacga ctccatctcg
      361 tgaatagttc ccggagcggc accgtcagca tgaaaccagc tctcgaagtg gcgacgatac
      421 gaacaattgg cgcggaaggc ataaatgtta ctgccgttaa cctgactggc gcggattact
      481 ggcgcatgac cttgtggcgt gacgatcatc agctcctcgc ggtataccgg tatcccgtca
      541 atggcagtat ggttaatggg cccatcaata aatgccgcat tcagttttcc ctccagcact
      601 ccttccagca tagtgccgga agggccggtg gaaagtgaaa actgaatttt gggataacga
      661 tggttgaatt ccgccagcgt ggccggaatg cgcactgctg cggtgctttc cagcgaacca
      721 agagaaaata aaccttgcgg ctcatcgcca gcgacaacgc tccgcgcttc atccactaac
      781 gcgagaattt gttggctata gcgtaaaaag ttatgcccgg caggagagag acgtaaacgc
      841 tgattctcgc gaataaacag atctaccccc agttctgttt ccagctggcg tagacggata
      901 gtgagattcg acggcacgcg aagcactatt gctgctgcct gggttatgct gccagcttcg
      961 gcaaccgcgt tgaacatctc cagttgggtc agatccataa tattctcgat tcgtgaataa
     1021 gtggcttaat attattcatt ttaaagcaag agtaaatctg cgtatcttca taccatgact
     1081 cataaaggag ataccccgat gaccattact ccggcaactc atgcaatttc gataaatcct
     1141 gccacgggtg aacaactttc tgtgctgccg tgggctggcg ctaacgatat cgaaaacgca
     1201 cttcagctgg cggcagcagg ctttcgcgac tggcgcgaga caaatataga ttatcgtgct
     1261 gaaaaactgc gtggtatcgg taaggctctg cgcgcccgta gcgaagaaat ggcgcaaatg
     1321 atcacccgtg aaatgggcaa accaatcaat caggcgcgcg ctgaagtggc gaaatcggcg
     1381 aatttgtgtg actggtatgc agaacatggt ccggcaatgc tgaaggcgga acctacgctg
     1441 gtggaaaatc agcaggcagt tattgagtat cgaccgttgg ggacgattct ggcgattatg
     1501 ccgtggaact ttccgttatg gcaggtgatg cgtggcgcgg ttcccatcat tcttgcaggt
     1561 aacggctact tacttaaaca tgcgccgaat gtgatgggct gtgctcagct cattgcccag
     1621 gtgtttaaag atgcgggaat cccgcaaggc gtatatggct ggctgaatgc cgacaacgac
     1681 ggtgtcagtc aaatgattaa agattcgcgc attgctgctg tcacggtgac cggaagtgtt
     1741 cgtgcgggag cggctattgg cgcacaggct ggagcggcac tgaaaaaatg cgtactggaa
     1801 ctgggcggtt cggatccatt tattgtgctt aacgatgccg atctggaact ggcggttaaa
     1861 gcggcggtag ccggacgtta tcagaatacc ggacaggttt gtgcagcggc aaaacgcttt
     1921 attatcgaag agggaattgc ttctgcattt accgaacgtt ttgtggcagc tgcggcagcc
     1981 ttgaaaatgg gcgatccccg tgatgaagag aacgctctcg gaccaatggc tcgttttgat
     2041 ttacgtgatg agctgcatca tcaggtggag aaaaccctgg cgcagggtgc gcgtttgtta
     2101 ctgggcgggg aaaagatggc tggggcaggt aactactatc cgccaacggt tctggcgaat
     2161 gttaccccag aaatgaccgc gtttcgggaa gaaatgtttg gccctgttgc ggcaatcacc
     2221 gttgcgaaag atgcagaaca tgcgctggaa ctggctaatg atagtgagtt cggcctttca
     2281 gcgaccattt ttaccaccga cgaaacacag gccagacaga tggcggcacg tctggaatgc
     2341 ggtggggtgt ttatcaatgg ttattgtgcc agcgacgcgc gagtggcctt tggtggcgtg
     2401 aaaaagagtg gctttggtcg tgagctttcc catttcggct tacacgaatt ctgtaatatc
     2461 cagacggtgt ggaaagaccg gatctgacgc gacatattca gctctgatat actcgcaggt
     2521 cttttcagac ctgcggtcca ggagtagaaa gtggcagtcg ccatggataa tgcaatttta
     2581 gaaaacatct tgcggcaagt gcggccgctc attggtcagg gtaaagtcgc ggattatatt
     2641 ccggcgctgg ctacagtaga cggttcccga ttgggcattg ctatctgtac cgttgacgga
     2701 cagctttttc aggccggaga cgcgcaagaa cgtttttcca ttcagtctat ttccaaagtg
     2761 ctgagtctcg ttgtcgccat gcgtcattac tccgaagagg aaatctggca acgcgtcggc
     2821 aaagatccgt ctggatcacc gttcaattct ttagtgcaac tggaaatgga gcagggtatt
     2881 ccgcgtaatc cgtttattaa tgctggtgcg ctggtggtct gcgatatgtt gcaagggcga
     2941 ttaagcgcac cacggcaacg tatgctggaa gtcgtgcgcg gcttaagcgg tgtgtctgat
     3001 atttcatacg atacggtggt agcgcgttcc gaatttgaac attccgcgcg aaatgcggct
     3061 atcgcctggc tgatgaagtc gtttggcaat ttccatcatg acgtgacaac cgttctgcaa
     3121 aactactttc attactgcgc tctgaaaatg agctgtgtag agctggcccg gacgtttgtc
     3181 tttctggcta atcaggggaa agctattcat attgatgaac ctgtggtgac gccaatgcag
     3241 gcgcggcaaa ttaacgcgct gatggcgacc agtggtatgt accagaacgc gggggagttt
     3301 gcctggcggg tggggctacc ggcgaaatct ggcgttggtg gcggtattgt ggcgattgtt
     3361 ccgcatgaaa tggccatcgc tgtctggagt ccggaactgg atgatgcagg taactcgctt
     3421 gcgggtatcg ccgtccttga acaattgacg aaacagttag ggcgttcggt ttattaatgc
     3481 agtctctcga tccactcttc gcgcgtttat cccgttcaaa atttcgctct cgctttcgtc
     3541 tgggcatgaa agagcgtcag tattgcctgg agaaaggcgc gccagtcatc gaacaacatg
     3601 cggcggattt tgtcgctaaa cgtcttgctc cggcattacc ggctaatgac ggtaagcaaa
     3661 cacccatgcg cgggcatccg gtgtttatcg ctcagcacgc caccgcgacc tgctgtcgcg
     3721 gttgtctggc taagtggcat aacattccgc agggtgtttc gttaagcgaa gaacaacaac
     3781 gttatatcgt ggcggtaatc tatcactggc tggttgtaca gatgaatcag ccatgagaat
     3841 tgcggaaaac gataaaatag cattcccagg gtacctttat ttgccgccgc tgttagcgaa
     3901 catgctatcc tttatgagaa tttacttatt acgttgagat aattgccacc cgcaattgta
     3961 tggacagata attaatgcat gtacacccca tttcaacttt ccgtttgttc caggaaggtc
     4021 atctgctacg taatagcatc gctatttttg cgctaaccac gctgttctat tttattgttg
     4081 cagagttacg gctggttcac gaactttctc ttttctggcc gctgaatggc gtaatggcgg
     4141 gggtgtttgc ccgctatgtc tggcttaatc gactgcatta ctatgcgatc agttatgtgg
     4201 cgatgctggt ttatgatgcc ataaccaccg aatggggact ggtttcactg gttatcaatt
     4261 tctccaatat gatgtttatt gttaccgtcg ccttactggt cgtgcgggat aagcgtctgg
     4321 ggaaaaataa gtatgagcca gtaagtgcct tacggctatt taattactgt ctgattgccg
     4381 cattattatg cgctattgtc ggggcgattg gttcggtcag tattgatagt ctggatttct
     4441 ggcctttgct tgcggactgg ttcagtgagc aattctcaac gggcgtgttg atcgtgcctt
     4501 gtatgctgac gttggctatt cctggagtgc tgccgcgctt taaagcagaa cagatgatgc
     4561 ccgccatcgc gcttattgtg tcggttattg cctcggtagt cattggcgga gcggggagtc
     4621 tggcgttccc gctgcctgca ttaatctggt gtgcagtgcg ctatacgccg caggtaacat
     4681 gtctgttgac ctttgtcacc ggtgcggtgg aaatcgtact ggtggcaaat tcggtgattg
     4741 atatctcggt cggttcgccg ttctccattc cggaaatgtt ctccgcacgt ctcggtattg
     4801 ccacgatggc gatatgccca attatggttt cttttagcgt ggcagcgatc aattcgctaa
     4861 tgaagcaagt tgcgctgcga gccgactttg attttctgac tcaggtttac tcacggtccg
     4921 gtctttatga ggcgctgaaa agtccatcgc tgaaacagac acaacatctg acggtcatgc
     4981 tgcttgatat cgactatttc aaaagcatta acgataacta tggacatgaa tgtggcgata
     5041 aagtgttaag cgtgtttgcc cggcatattc agaagattgt cggtgataag gggctggtgg
     5101 cgcgcatggg cggcgaagaa tttgctgttg cagtgccgtc ggtgaatcct gtcgatggtc
     5161 tgctaatggc ggaaaaaatc cgtaaaggcg ttgaactgca accattcacc tggcaacaaa
     5221 aaacgcttta tctcacggta agtattggcg tcggtagtgg tcgcgcatcg taccgaacgc
     5281 tgaccgatga ttttaataaa ttgatggtcg aagccgatac gtgtctgtat cgct
//
LOCUS       DAJTSL010000216         5199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTSL010000216 DAJTSL010000000
VERSION     DAJTSL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.218"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..107
                     /locus_tag="OO433_003824"
     CDS             <1..107
                     /locus_tag="OO433_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR6052127.1"
                     /translation="EQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(157..1905)
                     /locus_tag="OO433_003825"
     CDS             complement(157..1905)
                     /locus_tag="OO433_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCR6052128.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDYRLLVLLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPDIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFLITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(1905..2975)
                     /locus_tag="OO433_003826"
     CDS             complement(1905..2975)
                     /locus_tag="OO433_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6052129.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQRPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQMD"
     gene            complement(2965..4416)
                     /locus_tag="OO433_003827"
     CDS             complement(2965..4416)
                     /locus_tag="OO433_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6052130.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFTGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(4427..4873)
                     /locus_tag="OO433_003828"
     CDS             complement(4427..4873)
                     /locus_tag="OO433_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6052131.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1316 a         1342 c         1311 g         1230 t
ORIGIN      
        1 aagagcaact tcaggacgca aactcaagct ggatggtcga agactctttc ccgagagcat
       61 tacgcgagta caacgaaatg gttgatgact ataatagcct gcgttaaacg tttttgatga
      121 taaatgcagt gtcggatgcg gcgcatctga cacagttcac cccttgtgaa gtaactccat
      181 aaatgcctca tatcctgtta acccggcaat aacctctgcc ggatgctgac acaatatgct
      241 tgccagatag ctaaatattt ttagctcatg acgcgcatcg gcggcggcgc aggcgatcaa
      301 gacatggtta atgacttcgt tattcacctc aactggttgg gcgagggtaa taagaaatcc
      361 acgaaaacgt cgctcctgtt cgctccagca atgtgggatg gcgaggcgat taacaatcag
      421 gttttcacct tcgccttcgc gggcgatgat gcgttgtgct tcatcggcgg taatatggtg
      481 ttgtgcgact aattgcgcac aaatttgccg ggtaatatgt tgccagcttt caccgcgtac
      541 attcgaataa tgaaaacttc cgggggcaga gaaaaaacgc tccggttgct ggcgaataaa
      601 tgccgtcgcc agaaaatgtt ttatttgttc gataccggca gccgtaatga tattttttac
      661 ggtgatgtaa ttatttaccg cgtcttccag taaataatgg ctattgttaa tgatcaataa
      721 cggctcaatc tcttcgcgaa tggcaacaag ttcgcttaag ctacgggcaa taattacccg
      781 acaatttaaa acatcgcgct caatggcgag ctgattaata gtggcaatcg cgttctggtc
      841 cgagagcaaa atgatcggct ggcgttcgtt ttgatgccgc tccagcgcac aggcaaaata
      901 caaaccgatc agatcgctgt cgaaaagggg aatatcgagc tgttcacgca gtagcgtaat
      961 aaagtgcaga ctcatatcaa aggccgcagg ccaggctgct ttcaggttat tcatgctact
     1021 ttggcggtgc tcagcaatcc acaccggtgc ggcaaggcaa cgtaataaat gcccggtaat
     1081 attctcaacc aactgcgtat caataatatt caatgacgcg atgtctggca ccctttgcag
     1141 caaccccagc acatgatcgg ctgaaattac ctgcgcttgc tgatgctgtt tctctatcaa
     1201 acctgtcagg gttcttacag cgttatcacc aagaaacatg ccgctttgtt caacgatttg
     1261 ttttatctcg tcaccgggat attgcggcca ctcatcggtc agttgattac gcagggcgta
     1321 aatcgccaga atcaggctgg agagataatc accctgcatg agcggccagc ggtgttggtt
     1381 gtcgcaggcc gtggataaat gctgaaggtt gtctcgtgtt acgcccgcca gcggaattaa
     1441 aaacggatct ttgcgcagca agttcgccag caagataact cgcttctctt ctgtctcatc
     1501 aatgaaatgg cccaaaccgg ggcggctggc caggcaacaa gcgcgttcat aacgctgttt
     1561 taaccggggc agacgctctg cgacccacgt ttctggcaaa ttaagcgccg aggcgagttg
     1621 cgcacgggga gtaaaggtat tcagtaataa cagtaccagc agccgatagt cgttatcgtg
     1681 cttttgcagc aactgaaaaa agctgcggcg ctcgaagatt tccagctgat agcccgcact
     1741 accactggcg gaaatgcggg ctttgccgtt aagggtaaag ttgagatagt caatatcacg
     1801 caggatggtc ctgcctgaaa cgccagtctg ttgcgccagc tcaccaggcg tgcgcggctg
     1861 ttgctccagc agatcgacaa tctttaactg gcgttcgttt agcattaatc catctgacgg
     1921 aactggctaa acgcgttgac tttctccgcg gttaaggtgg tcagaaaatc ccgtatcaac
     1981 gtgagcagag catcgtaatc ggcttttgaa atcataccgc tgttagcgtg cagatagcgg
     2041 gttggcaggc acagcgcaac aaccggacgc ccgccgccca tcacgttgta gcgcccgcca
     2101 tccgtcgcac cggttttcat ggtggaaaat tgcagcggta aatcgttatg tgcggcacag
     2161 ctttttaacg ctgctaccag tttctggttg gggaagtagc gcttgtcaaa cagcatcagc
     2221 cccggcccct ggcccagttt cagcgggtat ttaatgttat caatgcccgg aacatcgcct
     2281 gcgacagcgg tatccagcac gatcactaca tccggtttaa tgtgttccgc cgaggtttgt
     2341 gccccgcgta gcccaacttc ttcttccacg ctgccgacgc catacagcgt aatttcggga
     2401 ttattcaccg tctgcaatag ttcagccatc attgcgcagc cgatgcggtt atccagcgcc
     2461 ttgccgacca ctttatcttc gccccagcag gcaaaattgg cttccgggct aataaaatcg
     2521 cccatcgcca caccgcgttt ttccacctca tcgcgactgt ttgcgccaat atcaatgaac
     2581 atctcatcaa atgacagcgg ccgttgcctt tgcttttccg ttaacgcatg aggcgcgacg
     2641 gaaccaatca caccagggat tttcactccc ttgtgtgtgc gtatggttac ccggtggttg
     2701 agcatcgact gattccacca gccaccaatg gtggtgaaac gcagaaaacc gctctcgtcg
     2761 atgtgggtga ccataaagcc gacttcatcc atatgcccga caacggcaac ttttggacct
     2821 ttattcccct tacgggcaac aaagctgccc agaccatcaa aggtgatctc attaacgcaa
     2881 ggttccagcg tgtttatcag aatgtcgcga acttcctgtt catcgccgct gacggcgctg
     2941 gcttcgcaca actgttgcag taactcaatg ttcatggaca agctcctgtt gcgcggcttt
     3001 cgctttgcgg cgtaaccaca atcctttcag gacgataatg aatgaaatgt tcagcgcgag
     3061 gccaatcgcc agcaccagat aaaacgaacc caccggcgac atcaggccga tcagcgggtc
     3121 aaagataccc aaaccaggtg ccagacgttt gattccgaaa gcgatcacca gcacgccggt
     3181 aatgccaccc gccagcgtat tggcggtaat catcggcaac ggagctgcca gagcgtaagg
     3241 tatagccggt tcggttgcga ctgtggcacc gaccacaatg gcactactgg cagcctcttt
     3301 ttcctgtcgg gtaaacagtt tgggcgcaat gaacgtcgca agacccgccg caacgggcgg
     3361 catcaacgcg acaacgccga cgatggcgta ccagtcataa atgtgttttt ccagcagtga
     3421 gaagcagaag aaccaggcgg ttttattgat ggggccgccc atatcgaacg ccagcatcgc
     3481 accgaccaga aacgctgctg caaacttcat tgatggagga atggtattca ggaagtggag
     3541 cagcccgccc atgaggtcgg acatcaccgg cccgataacg tagtaagtga gcacgccgaa
     3601 aaccagcaga gtaacgaacg ggatcagcat tgaaccgagc agcggttgta atgctttgcc
     3661 aaggcgcact ttgcggaacc agaagacgaa atagccaatc gccagcccca gcaccaccgc
     3721 gccgaggaat cccgcacccg actgggtgcc gagcagcgct ttgtcgttcg ccagatagca
     3781 aaccagaaac gccggagcga aagccggttt atcggcaatc gacgaggcaa tataagcgcc
     3841 catgatgggg atcataaagg tgaagccaag gtaaccgatg gattcaacta cccaggtaaa
     3901 cgacggcgcg cctttcgaca tatcggtata cggcaaaccg aactgcacca gcatgttggc
     3961 aagcgccacc aggataccac cgccgatgac aaacggcagc gccgcagaga cgcccgccat
     4021 caggtggctc atcacgctgc cgctttgcat ttcctgtttg ccaagcttca cgccactatc
     4081 tgcggcaaaa agctgcgagt ttgtcggtaa ttcgctgaaa atctggtcga tatttttcaa
     4141 cgcctgggag atggcaatct cataaacttt cttcccggta aaccgcgcgc gatcatcacc
     4201 gctcaggcca cgcccggtag cgagaatgac gtaatcggca gcggcaatct cttcgctgga
     4261 taagcggttt tcaacgccac tggacccttg agtttccact tttatggtat ggccgagaga
     4321 acgggctttc tgttccaggg cttccgccac catgtaggtg tgagcgatcc cggcggggca
     4381 gttggtgatc gcgacaatac gtaaggatga ctccatcgcc acttccttat gagctgaggg
     4441 tttgatttaa caaggcaagc acttggtcgg tatcgccctg ttgcagttga tgaataaatt
     4501 ccttgtgaat aattttgcga cacagtgtgc cgatgatttt gacctgatcc tcttcgccgc
     4561 tttgcggcac gccgaggcag atccagcaat tgacgtcttc gccatcgctg gctttccagt
     4621 caatagcctg cgctttgcgg gcgaataata cgaacggttg tttaacgcag gcgcttttac
     4681 cgtgcggcac ggcgacaccg gaaccaaatc cagtagagtg cattttttcg cgcagcagca
     4741 gggtttgcag gaactggtgt gagtcggtga taaaaccgtt ttgtaacgct attgtcgcca
     4801 actgcttcag aacggaataa gcgccattgc cctgaatatt caggtcaata cagcttgcag
     4861 taagagctgc cattaatcgg actccataaa atatatttta atcaattgta atggtggagc
     4921 gattaagtga tgagaggcga tatgtcggta aacaatgaca atttgagaca gagtgaaagg
     4981 tcagatttgc ggagtaatgc acataatggt tatttaaata aaccacatga atcattaatg
     5041 ggttattcat tatttttgtg atttactcac aatgtatcag ggaaatataa cttaccggga
     5101 gatgtaatat gtttaatttt tcaaatcgaa atttaaaata ttgtgccgga ggcatctccg
     5161 gcacattggg caattacggc aggtaaaaca cttcttgca
//
LOCUS       DAJTSL010000217         5056 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTSL010000217 DAJTSL010000000
VERSION     DAJTSL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.219"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..226
                     /locus_tag="OO433_003829"
     CDS             <1..226
                     /locus_tag="OO433_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6052132.1"
                     /translation="DVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLA
                     RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            385..789
                     /locus_tag="OO433_003830"
     CDS             385..789
                     /locus_tag="OO433_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6052133.1"
                     /translation="MGISLNHGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQ
                     QWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSL
                     YESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(767..1390)
                     /gene="tetR(B)"
                     /locus_tag="OO433_003831"
     CDS             complement(767..1390)
                     /gene="tetR(B)"
                     /locus_tag="OO433_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6052134.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1472..2677
                     /gene="tet(B)"
                     /locus_tag="OO433_003832"
     CDS             1472..2677
                     /gene="tet(B)"
                     /locus_tag="OO433_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6052135.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2790..3383)
                     /gene="tetC"
                     /locus_tag="OO433_003833"
     CDS             complement(2790..3383)
                     /gene="tetC"
                     /locus_tag="OO433_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6052136.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3516..3887
                     /locus_tag="OO433_003834"
     CDS             3516..3887
                     /locus_tag="OO433_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6052137.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(3897..>5056)
                     /locus_tag="OO433_003835"
     CDS             complement(3897..>5056)
                     /locus_tag="OO433_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR6052138.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1358 a          964 c         1104 g         1630 t
ORIGIN      
        1 tgacgtggtg atatggatga cggatggctg gccgctgtat gaatcccgcc tgaagggaaa
       61 gctgcacgta atcagcaagc gatatacgca gcgaattgag cggcataacc tgaatctgag
      121 gcagcacctg gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca
      181 tgacaaagtc atcgggcatt atctgaacat aaaacactat caataagttg gagtcattac
      241 cctgtggcga atatatcagc ttcaacggcg agcagtcatt tatctaaatt attagattgc
      301 cagctaatca cagtagtagc tcaaggcaag catcgttatt ttcggctagc aggaaaagat
      361 attgctgaat tgatggaaag tatgatgggg atctccttaa accatggcgt acatgccaaa
      421 gtttccacgc cagtgcattt acgaaaagca cgtacttgct atgatcattt agctggcgaa
      481 gttgccgtta agatctatga ttccctttgt caacagcaat ggatcactga aaatggttca
      541 atgatcacat taagtggtat tcaatatttt catgaaatgg gaattgacgt tccttccaaa
      601 cattcacgta aaatctgttg tgcgtgttta gattggagtg aacgccgttt ccatttaggt
      661 gggtacgttg gagccgcatt attttcgctt tatgaatcta aagggtggtt aactcgacat
      721 cttggttacc gtgaagttac catcacggaa aaaggttatg ctgcttttaa gacccacttt
      781 cacatttaag ttgtttttct aatccgcata tgatcaattc aaggccgaat aagaaggctg
      841 gctctgcacc ttggtgatca aataattcga tagcttgtcg taataatggc ggcatactat
      901 cagtagtagg tgtttccctt tcttctttag cgacttgatg ctcttgatct tccaatacgc
      961 aacctaaagt aaaatgcccc acagcgctga gtgcatataa tgcattctct agtgaaaaac
     1021 cttgttggca taaaaaggct aattgatttt cgagagtttc atactgtttt tctgtaggcc
     1081 gtgtacctaa atgtactttt gctccatcgc gatgacttag taaagcacat ctaaaacttt
     1141 tagcgttatt acgtaaaaaa tcttgccagc tttccccttc taaagggcaa aagtgagtat
     1201 ggtgcctatc taacatctca atggctaagg cgtcgagcaa agcccgctta ttttttacat
     1261 gccaatacaa tgtaggctgc tctacaccta gcttctgggc gagtttacgg gttgttaaac
     1321 cttcgattcc gacctcatta agcagctcta atgcgctgtt aatcacttta cttttatcta
     1381 atctagacat cattaattcc taatttttgt tgacactcta tcattgatag agttatttta
     1441 ccactcccta tcagtgatag agaaaagtga aatgaatagt tcgacaaaga tcgcattggt
     1501 aattacgtta ctcgatgcca tggggattgg ccttatcatg ccagtcttgc caacgttatt
     1561 acgtgaattt attgcttcgg aagatatcgc taaccacttt ggcgtattgc ttgcacttta
     1621 tgcgttaatg caggttatct ttgctccttg gcttggaaaa atgtctgacc gatttggtcg
     1681 gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg gattacttat tgctggcttt
     1741 ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt tcagggatca caggagctac
     1801 tggggctgtc gcggcatcgg tcattgccga taccacctca gcttctcaac gcgtgaagtg
     1861 gttcggttgg ttaggggcaa gttttgggct tggtttaata gcggggccta ttattggtgg
     1921 ttttgcagga gagatttcac cgcatagtcc cttttttatc gctgcgttgc taaatattgt
     1981 cgctttcctt gtggttatgt tttggttccg tgaaaccaaa aatacacgtg ataatacaga
     2041 taccgaagta ggggttgaga cgcaatcgaa ttcggtatac atcactttat ttaaaacgat
     2101 gcccattttg ttgattattt atttttcagc gcaattgata ggccaaattc ccgcaacggt
     2161 gtgggtgcta tttaccgaaa atcgttttgg atggaatagc atgatggttg gcttttcatt
     2221 agcgggtctt ggtcttttac actcagtatt ccaagccttt gtggcaggaa gaatagccac
     2281 taaatggggc gaaaaaacgg cagtactgct cggatttatt gcagatagta gtgcatttgc
     2341 ctttttagcg tttatatctg aaggttggtt agttttccct gttttaattt tattggctgg
     2401 tggtgggatc gctttacctg cattacaggg agtgatgtct atccaaacaa agagtcatca
     2461 gcaaggtgct ttacagggat tattggtgag ccttaccaat gcaaccggtg ttattggccc
     2521 attactgttt gctgttattt ataatcattc actaccaatt tgggatggct ggatttggat
     2581 tattggttta gcgttttact gtattattat cctgctatcg atgaccttca tgttaacccc
     2641 tcaagctcag gggagtaaac aggagacaag tgcttagtta tttcgtcacc aaatgatgtt
     2701 attccgcgaa atataatgac cctcttgata acccaagagg gcatttttta cgataaagaa
     2761 gatttagctt caaataaaac ctatctattt tatttatctt tcaagctcaa taaaaagccg
     2821 cggtaaatag caataaattg gcctttttta tcggcaagct cttttaggtt tttcgcatgt
     2881 attgcgatat gcataaacca gccattgagt aagtttttaa gcacatcatc atcataagct
     2941 ttaagttggt tctcttggat caatttgctg acaatggcgt ttaccttacc agtaatgtat
     3001 tcaaggctaa ttttttcaag ttcattccaa ccaatgatag gcatcacttc ttggataggg
     3061 ataaggtttt tattattatc aataatataa tcaagataat gttcaaatat actttctaag
     3121 gcagaccaac catttgttaa atcagttttt gttgtgatgt aggcatcaat cataattaat
     3181 tgctgcttat aacaggcact gagtaattgt tttttatttt taaagtgatg ataaaaggca
     3241 cctttggtca ccaacgcttt tcccgagatc tcatctattg aaacagcttg atagcctttt
     3301 tcaacaaaca atattcgtgc tgagttaacc agtgattgat aggtactctt aaaattttct
     3361 tgttgatgat ttttattttc catgatagat ttaaaataac ataccgtcag tatgtttatg
     3421 gtatcatgat gatgtggtcg tgacaatctt aagaacattt aggttatttt atgtatattg
     3481 aacagcattc tcgctatcaa aataaagcta ataacatcca attaagatat gatgataagc
     3541 agtttcatac aacggttatc aaagatgttc tattatggat tgaacataat ttagatcagt
     3601 ctttactgct tgatgatgtg gcgaataaag cgggttatac caagtggtat tttcagcggc
     3661 tgttcaaaaa agtaacaggg gtcacactgg ctagctatat tcgtgctcgt cgtttgacga
     3721 aagcggctgt tgagttgagg ttgacgaaaa aaactatcct tgagatcgca ttaaaatatc
     3781 aatttgattc ccaacaatct tttacacgtc gatttaagta catttttaag gttacaccaa
     3841 gttattatcg gcgtaataaa ttatgggaat tggaggcaat gcactgagag atcccctcat
     3901 aatttcccca aagcgtaacc atgtgtgaat aaattttgag ctagtagggt tgcagccacg
     3961 agtaagtctt cccttgttat tgtgtagcca gaatgccgca aaacttccat gcctaagcga
     4021 actgttgaga gtacgtttcg atttctgact gtgttagcct ggaagtgctt gtcccaacct
     4081 tgtttctgag catgaacgcc cgcaagccaa catgttagtt gaagcatcag ggcgattagc
     4141 agcatgatat caaaacgctc tgagctgctc gttcggctat ggcgtaggcc tagtccgtag
     4201 gcaggacttt tcaagtctcg gaaggtttct tcaatctgca ttcgcttcga atagatatta
     4261 acaagttgtt tgggtgttcg aatttcaaca ggtaagttag ttgctagaac ccatggctcc
     4321 tttgccgacg ctgagtagat tttaggtgac gggtggtgac aatgagtccg tgtcgagcgc
     4381 tgattttttc ggcctttaga gcgagattta tacaatagaa tttggcatga gattggattg
     4441 cttttagtca gcctcttata gcctaaagtc tttgagtgac tagatgacat atcatgtaag
     4501 ttgctgatag gtttccagtt ttccgctcct aggtctgcat attgtacttt tcctcttact
     4561 cgacttaacc agtaccaacc cagcttctca acggatttat accatggcac tttaaagcca
     4621 gcatcactga caatgagcgg tgtggtgtta ctcggtagaa tgctcgcaag gtcggctaga
     4681 aattggtcat gagctttctt tgaacattgc tctgaaagcg ggaacgcttt ctcataaaga
     4741 gtaacagaac gaccgtgtag tgcgactgaa gctcgcaata ccataagtcg tttttgctca
     4801 cgaatatcag accagtcaac aagtacaatg ggcatcgtat tgcccgaaca gataaagcta
     4861 gcatgccaac ggtatacagc gagtcgctct ttgtggaggt gacgattacc taacaatcgg
     4921 tcgattcgtt tgatgttatg ttttgttctc gctttggttg gcaggttacg gccaagttcg
     4981 gtaagagtga gagttttaca gtcaagtaat gcgtggcaag ccaacgttaa gctgttgagt
     5041 cgttttaagt gtaatt
//
LOCUS       DAJTSL010000218         5037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTSL010000218 DAJTSL010000000
VERSION     DAJTSL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.220"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..998
                     /locus_tag="OO433_003836"
     CDS             <1..998
                     /locus_tag="OO433_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCR6052139.1"
                     /translation="DIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDE
                     TLKPFVRDASGSRLKDLPKPNKSDDESRSNDAVNRYKLLKKDARIVAAQQVARQESAM
                     CLRRRWSPENFKLFLVEHPLVRHLTRRLIWGVYSAENQLQACFRVAEDNSYSTADDDL
                     FTLPEGGISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTGAERNASE
                     LTRWAGRKCPSGRVMGLANKGWIKGTPQDGGWIGWMIKPLGRWSLVMEIDEGFAVGMS
                     PAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVIDAITASELINDIEALFE"
     gene            1008..1250
                     /locus_tag="OO433_003837"
     CDS             1008..1250
                     /locus_tag="OO433_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR6052140.1"
                     /translation="MDKELPWLADNAQLELKYKKSKTPLSHRNWPGEPVSVITESIIQ
                     TLGDELLQKAEKKKRLSGVMKIFHWSGSPPSRRPST"
     gene            2774..4639
                     /locus_tag="OO433_003838"
                     /pseudo
     CDS             2774..4639
                     /locus_tag="OO433_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            4701..>5037
                     /locus_tag="OO433_003839"
     CDS             4701..>5037
                     /locus_tag="OO433_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052141.1"
                     /translation="MIVQKELVAIYDYEVPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVNPLINDALIYIRDEFIDERKLRGESPEPVPVIFNCELQNIFNQ
                     LIEYYFIKVG"
BASE COUNT         1304 a         1271 c         1287 g         1175 t
ORIGIN      
        1 ccgacattgc cgagagccgc gaactcacgg tggcggagct tgaagatcgg ttagcaccgg
       61 atctcggtct ggatgataac ggttcgttgc tgctggattt cggcccacgc cagttcaccg
      121 tcagctttga tgaaacctta aaaccgtttg tgcgtgacgc ttccggcagc cgcctgaaag
      181 acctgcccaa accaaacaaa agcgatgatg aatcgcggtc gaacgatgcg gttaaccgct
      241 acaaattgct gaaaaaagat gcgcgtatcg tcgccgccca gcaggtagca aggcaggaat
      301 ccgccatgtg cctgcgtcgc cgctggtcgc cagaaaactt taagctcttc ctggttgagc
      361 atccgttggt tcgccactta acccgccgtc tgatttgggg cgtttatagc gccgaaaacc
      421 agctacaggc gtgctttagg gtggcggaag ataacagcta cagcaccgct gacgatgatc
      481 ttttcaccct gccggaaggc ggtatctcta tcggcattcc tcacgttctg gaaatatcac
      541 cgacggatgc tgccgccttt ggtcagcttt ttgccgacta cgaactgcta ccaccattcc
      601 gtcagcttga ccgtaacagt tacgccctga caggagccga gcgcaacgcc agcgaactga
      661 cccgctgggc aggcagaaaa tgcccgagcg gtcgggtcat ggggctggcg aataaaggct
      721 ggataaaggg caccccgcag gatggaggct ggatcggctg gatgatcaaa cccctgggcc
      781 gctggtcgtt agtcatggaa atcgatgaag gttttgcggt aggcatgtcg ccagccgaac
      841 tcagcgctga gcagctctta agcaagctgt ggctatggga aggcaaagca gaaagctatg
      901 gctgggggag taattcaaca caggaagcgc agttctccgt aatcgatgcc atcaccgcca
      961 gcgagctaat taacgatatt gaagccctgt ttgaataagg aaagagcatg gacaaggaat
     1021 taccgtggct ggcggataac gcccaactgg aactgaaata taaaaaaagc aaaacgccgc
     1081 tcagtcatcg caattggccg ggcgaaccgg tgtctgttat cactgaaagc atcattcaga
     1141 cactgggtga tgaattgcta caaaaagctg agaagaaaaa aagattgtct ggcgttatga
     1201 aaatttttca ctggagtggc agtccgccat cacgcaggcc atcaacttga tcggcgaaca
     1261 caaaccctca gtcccggccc ggacaatggc ggcgctagcc tgtatcgcgc aaaatgacag
     1321 ccaacagttg ctcgacgaaa tcgtccaaca agaggggctg gaatatgcga ctgaggtggt
     1381 aattgcacgc ctgtttattg cgcggtgtta tgagagtgat cctctggtag tgacattgca
     1441 gtatcaggac gaggattatg gctatggtta tcgctcagaa acctataacg aattcgatct
     1501 ccgactgcgt aagcatctct ctctggcaga ggaaagctgc tggcagcgtt gcgccgacaa
     1561 actcattgcc gcactaccag gaataaccaa agttcgccgc ccttttattg cgctgatcct
     1621 cccggaaaaa ccagaaatag ccaatgagtt ggtaggcctt gaatgcccgc gaactcattt
     1681 tcattctaag aagtggttaa aagttgttgc taatgacccc acagcggtga gaaaactcga
     1741 acactactgg agccaggata tatttagcga tcgagaagcc agctacatgt cgcatgaaaa
     1801 ccacttcggc tacgcggcct gcgccgccct tttgcgcgaa caaggactgg cagccattcc
     1861 gcgcctcgcg atgtatgccc ataaagaaga ttgcggcagt ctgctggtac aaattaacca
     1921 tccgcaagtc atccgcacct tgctactggt ggctgataaa aacaaaccca gcctgcaacg
     1981 tgtagctaaa taccataaaa acttccccca tgcgacgctc gccgcactgg cagaactgct
     2041 ggcgttaaca gaaccaccag cccgccctgg ttatccaatc atcgaagaca aaaagctgcc
     2101 tgcacagcaa aaagcacgcg atgaatactg gcgtacgctg ttacaaacgc tgatggcatc
     2161 gcagccacaa ctggcagaag aggtgatgca gtggttaagt actcaagcca gggcagtgct
     2221 gaatagttat ttatcggcac cgcccaaacc ggttattgat agtaccgata acagcaatct
     2281 gcctgaaatc ctcgtttcgc taccgtggcg tagtaagaaa aaaatgacag ctccacgtct
     2341 tgatttggca ccgctcgaat taactccgca agtttactgg caaccaggcg aacaagagag
     2401 gcttgccgcc actgagtctg cccgttattt cagcacggaa tctcttgcgc aacgcatgga
     2461 acaaaaaagt ggacgagttg tattacagga actgggtttt ggggatgatg tatggctgtt
     2521 tctgaattat atactccccg gaaaactgga tgctgcacgc aattcactca ttgttcagtg
     2581 gcattactac caggggcggg ttgaagagat cctgaatggc tggaactccc cggaagcaca
     2641 attagcagaa caggcgctcc gcagcggtca catagaagcg ttaattaaca tatgggaaaa
     2701 tgacaactac tcacgttatc gtccggaaaa gagtgtctgg aacctgtatt tattggcaca
     2761 gttgccgcgt gagatggctt tgaccttctg gctgcgtatc aatgagaaaa agcatctgtt
     2821 cgcgggtgag gactattttc tcagtatcct cggattggat gcgctaccag gtctgctgtt
     2881 ggctttttca catcgtccaa aagaaacatt tccgttaatt ttaaattttg gcgcaacaga
     2941 actggcgctg cctgttgccc gcgtctggcg acgtttcgcg gcgcagcgtg atctggctcg
     3001 ccagtggatt ttacaatggc cggaacatac ggctagtgca cttatccctc ttgtctttac
     3061 caaacccagc gataatagcg aagccgcatt acttgccctg cgtttactgt acgaacaggg
     3121 acatggcgaa ttgctacaaa ccgtggcaaa ccgctggcag cgtacagatg tatggtctgc
     3181 cctggagcag ttgcttaaac agggtccaat ggacatttac ccggcacgca ttccaaaagc
     3241 ccctgatttc tggcatccgg caatgtggtc caggcctcga cttatcacta ataatcagcc
     3301 tgttaccggt gacgctctgg aaattatcgg cgaaatgctg cgctttacct aggggggacg
     3361 tttttatagc gggctggaac aactgaaaac gttctgccag ccacaaacgc tggccgcttt
     3421 tgcctgggat ctcttcactg cctggcaaca agcgggggcc cccgcaaaag acaactgggc
     3481 atttctggca ttacgtctct ttggtgacga aagcacggca cgggatctga cgacacagat
     3541 cctcgcctgg ccacaagaag gcaaatctgc ccgtgctgtc agtggtctga atatacttac
     3601 cctgatgaat aatgatatgg cgctgatgca gctgcatcat gtatcacaac gggcgaaatc
     3661 acgcccctta cgtgaaaacg cagcggaatt tcttcaggtg gtcgcagaaa atcgcgggct
     3721 aagccaggaa gagttagcgg acagattagt cccaaccctg ggccttgatg atccgcaggc
     3781 gttgagtttt gattttggtc cccggcagtt taccgttcgc ttcgatgaaa acctcaaccc
     3841 ggttatcttt gatcagcaaa acgttcgcca gaaaagcgtt ccccggttgc gcgccgatga
     3901 cgatcaactg aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa aagatgctac
     3961 tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc ctacgtacca cccgacgctg
     4021 gtcgctggca gattttcatt ctctgtttgt taatcatccc tttacccgtc tggttaccca
     4081 gcgattaata tggggggttt atccggcaaa tgaaccgcgt tgtttactca aagcctttcg
     4141 tgtggccgca gagggggagt tctgcaatgc gcaagatgag ccaattgacc tgcctgcgga
     4201 cgctctgatt ggcattgccc acccgttaga aatgacagta gaaatgcgca gtgaatttgc
     4261 acagcttttt gccgattacg aaattatgcc gccttttcgc caattgtcgc gccgcacggt
     4321 gctgctcaca cctgacgagt caaccagtaa cagcctgact cgctgggaag gtaaatccgc
     4381 taccgttggg cagcttatgg gaatgcgata caaaggctgg gagtcaggtt atgaggacgc
     4441 atttgtctat gacctgggtg agtaccggct ggtccttaag ttttcacccg gttttaacca
     4501 ctacaatgtt gatagcaaag cgctaatgag cttccgttct cttcgagtgt accgtgacaa
     4561 taaatccgtc acttttgccg aacttgatgt gtttgatttg agtgaggcgt taagcgcacc
     4621 tgacgtcatt ttccattaaa aacacagcgg gcagtgatgc aactgcccgt tatcaacacg
     4681 acttaccagc ggggaaagcg atgatcgtgc aaaaagagct ggttgctatt tacgattatg
     4741 aggtccctgt acctgaaaat ccgttttcct tcagacttga gatccataaa tgctctgaat
     4801 tatttacagg ttccgtctat cgactggaac ggttccggct acgtccaaca tttcatcaac
     4861 gtgatcgaga ggatgttaac ccgctaataa atgatgcgtt gatttatata agagatgagt
     4921 ttattgatga gcggaaatta cgaggtgaat cacctgaacc tgtaccagta atttttaatt
     4981 gtgaactaca gaatatattc aaccaactaa tagaatatta ctttattaaa gtgggaa
//
LOCUS       DAJTSL010000219         4988 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTSL010000219 DAJTSL010000000
VERSION     DAJTSL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.221"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1249)
                     /gene="dhaR"
                     /locus_tag="OO433_003840"
     CDS             complement(<1..1249)
                     /gene="dhaR"
                     /locus_tag="OO433_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6052142.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWSVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAH"
     gene            1477..2586
                     /gene="dhaK"
                     /locus_tag="OO433_003841"
     CDS             1477..2586
                     /gene="dhaK"
                     /locus_tag="OO433_003841"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6052143.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLGNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWDKLGESNVTEQNSNC"
     gene            2558..3190
                     /gene="dhaL"
                     /locus_tag="OO433_003842"
     CDS             2558..3190
                     /gene="dhaL"
                     /locus_tag="OO433_003842"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6052144.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            3201..4619
                     /gene="dhaM"
                     /locus_tag="OO433_003843"
     CDS             3201..4619
                     /gene="dhaM"
                     /locus_tag="OO433_003843"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6052145.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLYTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDYIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         1248 a         1272 c         1239 g         1229 t
ORIGIN      
        1 cgtgcgccag ttccagccga ctcagacggc cattttcatt gtccgtacga tcgccaccaa
       61 taaattcttc cgccagtgcc gcatcaccat ataactcaca attgacggcg atataaggac
      121 ctgcagcacg ctcgctttca ttatgaattg cctgacttag cagtgccttg cccacgccct
      181 cttctccaca aagcaggaca ggaaagctac tgcgcgccgc ctggcgacca aaatgaatca
      241 agcggcgggt ttgcggatcg tcctgtggca tatgagcaaa ggtatggctg acttttccta
      301 attgactggt catcaactgc cgcatctgtt ccacaggatg gagcaacaaa ataaagctgg
      361 ttccctgcgt ttctattatc ggtttaaggg ttatcaccgc atcaataaac tgatgctgac
      421 tttcaaaggt tgcttctacg tgtttgagcg gatgtgcctg ttttattgct tgttgcaata
      481 cggcgggtaa cgttaagagt tcagtgattg cccgtccctg acttgccgtc gcgtcaaggc
      541 gcaagacccg cgccgcctgg gcattgataa attgcaaatt accctgctcg tcccagctaa
      601 tcacgccatc atccatactt tctaacaggg cattaagttg atttaaatga cggttagttt
      661 cagcgagcaa actgtccgtc agcagtaaat ttccgacctc gcgggcgatt gccaacgtca
      721 acggcaaatc agctgcggta gtttgctcaa ccggacacgc cagcgctatt gttcccgtca
      781 atcggccctt gctgtcaaac aacggcgttg cacaaaaggc ccagttccag agtgcctgtt
      841 tgaaatgttg atcggccatc gttttcacgg cctgaccaga gatagccgct aacgatagcg
      901 cacaagtacc aataattccc tcggcgcaat acgtgccgtc atggaatcca agtgcactta
      961 gctgctgcaa ggtttgcgga tcgccattac ggctgagaat gcaggcggtt tcatcgagga
     1021 taaacagcgc acactctcgc ggtgccatat attcccaggc atcttccagc gcagcctgcc
     1081 cgagcgtcag catcgctttc ttacgccgat aaatagaagc aaatgtcacg ccctgggcct
     1141 gatgtggtac gctccatgtc tcccgtttca tcagcttatt gcatcgctcc caggaggttg
     1201 caattaaggg agatatgccc cgaccatcgt tgttaaaagc gccactcata tcccgcatcc
     1261 cttatgacaa aaggtgttcc ggctcgcaat tttaagtatt aatacacgta aaagtttcaa
     1321 tatggaacat tcattaaagc atatgtttca ctatgaaaca taatttgggc taattttctt
     1381 ttcccttgcc tgatgcacaa tggattcatc ggcagtcagt ggtcgccgtg tcgttgaaca
     1441 tcatccatgc cctaccgtaa ttgctggagc aaaataatga aaaaattgat caatgatgtg
     1501 caagacgtac tggacgaaca actggcagga ctggcgaaag cgcatccatc gctgacactg
     1561 catcaggatc cggtgtatgt cacccgagct gatgcccctg ttgcaggaaa agtcgccctg
     1621 ctgtcgggtg gcggcagcgg acacgagccg atgcactgtg ggtatatcgg tcaggggatg
     1681 ctttcggggg cctgtccggg cgaaattttc acctcaccga cgcccgataa aatctttgaa
     1741 tgcgccatgc aagttgatgg cggcgaaggt gtactgttga ttatcaaaaa ttacaccggc
     1801 gatattctta actttgaaac agcgaccgag ttactgcacg atagcggcgt aaaagtgacc
     1861 actgtggtca ttgatgacga cgttgcggta aaagacagtc tttatactgc cgggcgacgc
     1921 ggcgttgcca acaccgtatt aattgaaaaa ctcgtaggcg cagcggcgga gcgtggcgac
     1981 tcactggacg cctgtgcgga actggggcgt aagttgaata atcaaggcca ctcaataggt
     2041 atcgctctcg gtgcctgtac cgttcctgcc gcgggcaaac cttcttttac cctggcggat
     2101 aatgagatgg agtttggcgt cggcattcat ggtgagccgg gtattgaccg ccgctccttc
     2161 tcttcccttg atcaaaccgt cgatgaaatg ttcgacaccc tgctgggaaa tggctcatac
     2221 catcgcactt tacgtttctg ggattatcaa caaggcagtt ggcaggaaga acaacaaacc
     2281 aaacaaccgc tccagtctgg cgatcgggtg attgcgctgg ttaacaatct tggcgcaact
     2341 ccgctttctg agctgtacgg cgtctataac cgcctgacca cacgttgcca gcaagcggga
     2401 ttgactatcg aacgtaattt aattggcgcg tactgtacct cactggatat gaccggtttc
     2461 tcaatcacct tactgaaagt tgatgacgaa acgctggcac tctgggacgc cccggtccac
     2521 accccggccc ttaactggga taaattagga gaaagcaatg tcactgagca gaactcaaat
     2581 tgttaactgg ctcactcgtt gtggcgatat tttcagcacc gagagcgagt atcttaccgg
     2641 actggatcgc gaaattggcg acgctgacca cgggctaaat atgaaccgag gctttagcaa
     2701 agtggtggaa aaactccctg ctatcgcaga taaagatatc ggtttcattc tcaagaatac
     2761 cggtatgacg ctgctttcca gcgtcggtgg tgccagtggt ccgctgttcg gtaccttctt
     2821 tatccgcgcc gcacaggcga cccaggcacg gcaaagcctg acactggaag agctttatca
     2881 gatgttccgc gatggcgcgg acggcgtaat cagtcgcggg aaagccgaac ctggcgataa
     2941 aaccatgtgt gatgtgtggg tgccggtggt ggaatcgtta cgtcagtcca gcgagcaaaa
     3001 tctctctgtt ccggtggcgc tcgaagctgc cagtagcatc gccgaatccg ctgcacaaag
     3061 tacgattacg atgcaagccc gcaaaggccg cgccagttat ctcggtgaac gcagtattgg
     3121 tcaccaggat cccggcgcga cctcggtgat gtttatgatg caaatgttgg cgttagccgc
     3181 aaaagagtaa ggaattggtg atggtaaacc tggtcatagt ttcacatagc agccgactgg
     3241 gagaaggtgt cggtgaatta gcccgtcaga tgttaatgag tgatagttgt aaaatcgcca
     3301 ttgccgcggg aattgacgat ccacaaaatc ctattggtac cgatgccgtc aaagtgatgg
     3361 aggccatcga atctgttgct gatgccgacc atgtgctggt catgatggat atgggtagcg
     3421 cattattgag tgctgaaact gcgctggaat tgctggctcc caagatcgcc gcaaaagtac
     3481 gtttgtgtgc tgcgccgttg gtcgaaggta cactggcagc aacggtcagc gcggcctcgg
     3541 gagcggatat cgacaaagtt atctttgacg ctatgcatgc gctggaagcc aaacgtgaac
     3601 aactgggttt accgtcctcc gacactgaaa tctctgacac atgtcctgcg tacgatgaag
     3661 aagcccgttc tctggcggtg gtcataaaaa accgtaacgg cctgcatgta cgtccggcct
     3721 cccggctggt ttatacctta tcgacattta atgccgatat gttgctggaa aaaaacggca
     3781 aatgcgtcac accagagagt attaaccaga ttgcgttact acaagttcgc tataacgata
     3841 cgctgcgcct gattgcgaaa gggccagaag ctgaagaggc actgatcgct ttccgtcagc
     3901 tggctgaaga taactttggt gaaacggagg aagtcgctcc acctactctg cgtcccgttc
     3961 cgcctgtttc gggtaaagcc ttttattatc aaccagtttt atatacggta caggcaaaat
     4021 caaccctgac cgtggaagaa gaacaagatc gattacgcca ggctattgac ttcacgttat
     4081 tagatctgat gacgttaaca gcgaaagcag aagccagcgg gcttgactat attgccgcaa
     4141 tcttttctgg tcaccataca ctgttagatg atccggaact gctggcggcg gcaagcgaac
     4201 tccttcagca tgaacattgc acggcagaat atgcctggca gcaagttctt aaagaactta
     4261 gccagcaata ccagcaactg gatgatgaat atctacaagc tcgctatatt gatgtggacg
     4321 atcttctgca tcgcaccctg gtccacctga cccaaacgaa agaagaactc ccgcagttta
     4381 actcgccaac tattctactg gcggagaaca tttatccttc cacagtactg caactggatc
     4441 cggcggttgt aaaaggtatc tgccttagcg ccggaagtcc ggtatcccac agcgccctaa
     4501 tcgcccgtga actggggatt ggctggattt gccagcaggg tgagaaactg tatgcgatac
     4561 aaccagaaga aacgctaacg ctggacgtta aaacgcaacg tttcaaccgt cagggttaag
     4621 ccactgttcg gatggcatcg ttctgatgtc atccggcaat ttactcagca ctaatggttt
     4681 ttaactacct gttatatttt tcaccaattt atgatgattt ttcttcgcta atcacgaacc
     4741 caacgccgct ggctggctaa taaacagacg ttatttgatc actctatttc gccgttattt
     4801 atccctttaa ttcccttttc taaaatgcct gacagttcgc agaatgagat ttcgatcatg
     4861 cagctagtgc gatcctgaac taaggttttc tgatacttga ataccgtttt attcggtttc
     4921 gccaaaggag aatgattgat gaaatccccc gcaccttctc gcccgcaaaa aatggcgtta
     4981 attccagc
//
LOCUS       DAJTSL010000220         4862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTSL010000220 DAJTSL010000000
VERSION     DAJTSL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.223"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            148..264
                     /locus_tag="OO433_003844"
     CDS             148..264
                     /locus_tag="OO433_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6052146.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            628..1470
                     /locus_tag="OO433_003845"
     CDS             628..1470
                     /locus_tag="OO433_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6052147.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            1473..2561
                     /gene="capU"
                     /locus_tag="OO433_003846"
     CDS             1473..2561
                     /gene="capU"
                     /locus_tag="OO433_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6052148.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTQKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2566..3516
                     /gene="virK"
                     /locus_tag="OO433_003847"
     CDS             2566..3516
                     /gene="virK"
                     /locus_tag="OO433_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6052149.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSSSGFDREGELMLSLFYNKTVITRLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQDLN"
     gene            3581..4525
                     /gene="lpxM"
                     /locus_tag="OO433_003848"
                     /gene_synonym="msbB"
     CDS             3581..4525
                     /gene="lpxM"
                     /locus_tag="OO433_003848"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6052150.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRYNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(4679..>4862)
                     /locus_tag="OO433_003849"
     CDS             complement(4679..>4862)
                     /locus_tag="OO433_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6052151.1"
                     /translation="IYQPVYSPWVNHAERLWQALHDTITRNHQCRSMWQLLKKVRHFM
                     ETVSPFPGGKHGLAKV"
BASE COUNT         1386 a          923 c         1116 g         1437 t
ORIGIN      
        1 tatcggtggc aatagcctgt gcaaattttt tataacccgg gatcgtgtca ggattcagag
       61 tatgataaag acagattttc atggctgccg gtttccgtcg tgcaaaaaac atgctgattt
      121 tacgaaggaa tgccttactg aacaaccttg cccggaatac cccccgaaat cagatggaac
      181 aatcagaaaa atgctctgca gttatggaat aaagaaggtg gggtggtacg ccgggagggc
      241 gatccgctgg aaatggcgca gtagctgcag gcctgtcatg atgccggaat agaagttcgt
      301 gtacaggtta atgaaagcgt caccccgtaa ataaactctg cgctgaaatt cctttttttc
      361 attggagcac ctgtgttgtt ctgaggtgaa catatcacct ctgttcaggt agccaaattc
      421 agtgtgccac ctcaaggttc ttagtgagtc gtttttaaaa gatatgataa tatatcattg
      481 tttatgaatt gttgatgttt aatttagtaa aatatatcaa gtatgccatg ggtttgctat
      541 gttattcatc cagtatctgg aaataaaatg caccagcttt attgggtttt ccttttgtct
      601 ctgcttacta ggtgccagaa actttaaatg ttaaacgaag ggggcatttt gttcaaagcc
      661 aatcatgttc cggtattaat gtatcatcat gtcagtcatt gccccggact ggttacactt
      721 tctcccgtga cattccggaa gcaaatgaaa tggttggctg aaaataactg gaaaacactg
      781 tcatcagatg aactggagtt tttttatcgg ggcggaaaac tcccccggaa aagcgttatg
      841 ttgacatttg atgatggata ccttgacaac tggtttcagg tatatccttt attaaaagag
      901 tttaatctca aagcacatat ttttttaatt accggtttta ttgggaatgg tccggttcgt
      961 cactctcctg gaaaggaata ttctcatcgc gattgcgaac atcaaatagc gacagggaat
     1021 gctgataatg ttatgcttcg ctggtctgaa gtaaacgaaa tgttgcaatc tggtctggtc
     1081 gaatttcatg ttcatacgca tactcataca cgctgggata aaaagttcag ttcacgagaa
     1141 gaacagtgta aacatcttcg gcaggacttg ttgagtggga gagaatattt gaaagaaatg
     1201 acaggaaaat gcagtaaaca tttgtgttgg ccggaaggtt attataacaa ggattatatt
     1261 caggtcgctg aagaacttgg attctattat ttatatacaa cagaaagaag aatgaatgct
     1321 cctgctaaag gaactacacg gataggaaga ataagcacca aagaacggga aagttgcgca
     1381 tggctgaaaa gacgactttt ttattataca acaccgtttt tttcatcgtt gctggccttt
     1441 cataaggggc caagattacc ggatgattaa taatgaatat actatttacg gaatcatcac
     1501 cgaatatagg tggtcaggaa ttacaggctg ttgctcaaat gaaggccctg aagaaaatgg
     1561 ggcattcagt tctgcttgtc tgcagggaaa acagcaaaat tgcttttgaa gccagtaaat
     1621 tgggaattga tatcacattc gcgttatttc gaaacagtct tcacatccct actgcatgga
     1681 gattactcgg aatagttcat ggttttcagc ccaatgcaat cgtttgtcac agtgggcatg
     1741 atagcaatat tgttggttta gtacggttat tcacttggaa acatccattc agaattatca
     1801 ggcaaaagac atatttgaca caaaaaacaa aagttttttc aataaatcat ttttgcgatg
     1861 aggtgattgt tcccggaaca agtatgaaga cacatctgga gcaggaagga tgtcgaaccc
     1921 gggttactgt tgtgcctcca ggctttgatt tccagaaatt atacgttgat tcccgaaaca
     1981 gtttgcctcc aaatgttctt tcttggctgg cgtcccgaag gggatgcccc gttattgctc
     2041 aggtaggaat gttgcgcccg gaaaaagggc acgaatttat gttgaattta ctgttccatt
     2101 taaaaatgaa tggacgacag ttctgttggt tgattgtggg gtctggttcg cctgaactgc
     2161 gggagcattt acagtatcag attgacagta tgggcatgca tgatgatgtt tttattgctg
     2221 acaatgtttt tcctgccgcc cccgtatatc gggttgccag tctggtggtt ctgccttcag
     2281 aaaacgaatc gtttggtatg gtactggcag aagcatcggc attttctgtg cctgtactgg
     2341 ccagtcagat tggtggaatc cctgatgtta ttcagaacaa ccagaccggg acattgttac
     2401 cagcaggtaa taagcacgca tggatgtgcg ccctgaatga tttttttaat gaccctgggc
     2461 gtttttatca gatggctcgc caggcaaaac aggatataga agagcggttt gatattaata
     2521 aaactgcgtt aaaaatactc acattagcga agcacaagta acaatatgtt ttctgtaagt
     2581 aacttatcat ttatcggttt ccttaaaagg attgtttttt cctcagattc actcccgggg
     2641 aagtgggaac acagaaaatt ccggttcatg tacattttgc gatgtgctat aaatccggtt
     2701 gccagtattc gatattatta cgaactgcgt tccttgccgt gcattgagga tattctggca
     2761 atacacccca cgttgccagc aagaattcat cgcccttatt tgcataaagg ggggcgcgcc
     2821 tggacccgtg gacaatatat tcttgagcat tatcgctttg tgcaaaattt gccagaaaaa
     2881 tattctaaat ttttgtttcc acaaaaatcg gtatcattag tccaattcat cgggaaagat
     2941 ggagagaatt ttgatatcca gtgttcttcc agtggttttg accgggaagg agagttgatg
     3001 ttgtcattgt tttacaataa aacagttatt acccggttaa cattttcagt cattctgact
     3061 caaaatggac acattgcttt tattggcgga ttacagggag cccctaaaaa taccggacct
     3121 gatgttattc ggtgcgcaac ccgggcctgt tatgggattt tcccaaaacg gattattttt
     3181 gaagcatttt gtgcgttgat gaaagcttgc aatatctctg agtgtctggc agtcagtgag
     3241 cacagtcatg ttttccggca attgagatac tggtatcaga aacgcaaaac ctttgttgcg
     3301 gtctatagtg atttctggga gtctgtagca ggaaaaacct gtggtgactg gtacagatta
     3361 ccaacccagg tgattcgaaa gccgctaagc gatatagcca gtaaaaaacg ctctgagtac
     3421 cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca
     3481 tatcctgtac actcagaaca tcaggattta aattaaggtt tgatgtattc tctgaataaa
     3541 acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga
     3601 gtttattcct gaatttaaga aaaattatct ttcccctgtt tactggtcta catggttcct
     3661 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
     3721 aaaaataggg cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg cgacgattaa
     3781 tttatcgctt tgtttcccag aaaagagtga tacagaacgg gaaataattg tcgacaaaat
     3841 gtttgccaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga
     3901 aaagttccag aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa ttcgatacaa
     3961 taacagaaat gtgatttttc tggttcccca tggctggagc gtggatattc ctgcaatgtt
     4021 gctggcagct cagggggaaa aaatggccgc catgtttcat cagcaacgaa atccagtgat
     4081 tgattatgtc tggaattctg tacggcgtaa attcgggggg cgcttacatt cccgggagga
     4141 tgggataaaa ccatttattc agtcagtacg ccagggatac tgggggtatt accttccaga
     4201 tcaggatcat ggtcctgaat acagtgaatt tgctgatttt tttgcgacct ataaagcgac
     4261 attaccaatt attggacgtc tgatgaacat cagtcaggct atgattatac cgcttttccc
     4321 ggtttatgat gaaaaaaaac atttcctgac tattgaagtt cggccaccaa tggatgcatg
     4381 cattgccagc gcggacaata aaatgattgc ccgacaaatg aacaaaacag tggaaatttt
     4441 ggtggggtca catccggaac agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc
     4501 aaacgaagcg gacccgtacc cttgaaatta taaagtacag gtatttccac tagttgtttc
     4561 ttacaggtta ccaatcgaaa cacatccccc ttccgtgtgt tgcccgtttg gcacggagga
     4621 ctgtgtagat gcagttcgtt aaatttaatt tatgcctaaa tagctgcgct taataccgct
     4681 acacttttgc cagtccatgt ttgcctccgg ggaatgggct gacggtttcc ataaaatggc
     4741 gaactttttt caacagttgc cacattgagc ggcactgatg attacgcgtt attgtgtcgt
     4801 gaagtgcctg ccatagccgt tcagcatgat tcacccatgg cgagtaaacc ggctgataaa
     4861 tg
//
LOCUS       DAJTSL010000221         4854 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTSL010000221 DAJTSL010000000
VERSION     DAJTSL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.224"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1250)
                     /locus_tag="OO433_003850"
     CDS             complement(<1..1250)
                     /locus_tag="OO433_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGCS family amino acid carrier protein"
                     /protein_id="HCR6052152.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPRAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMA"
     gene            1529..2482
                     /gene="tal"
                     /locus_tag="OO433_003851"
     CDS             1529..2482
                     /gene="tal"
                     /locus_tag="OO433_003851"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6052153.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2597..3184
                     /gene="mog"
                     /locus_tag="OO433_003852"
     CDS             2597..3184
                     /gene="mog"
                     /locus_tag="OO433_003852"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6052154.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSVLTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(3219..3785)
                     /gene="satP"
                     /locus_tag="OO433_003853"
     CDS             complement(3219..3785)
                     /gene="satP"
                     /locus_tag="OO433_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6052155.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YSGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3934..4647)
                     /gene="msyB"
                     /locus_tag="OO433_003854"
     CDS             complement(3934..4647)
                     /gene="msyB"
                     /locus_tag="OO433_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6052156.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAFDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(4673..>4854)
                     /locus_tag="OO433_003855"
     CDS             complement(4673..>4854)
                     /locus_tag="OO433_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6052157.1"
                     /translation="ARSASQSLNIPSEIKEGQTTDWININSDNDNKRCVSKITFSGHT
                     VNSSDMATLKIIGDD"
BASE COUNT         1275 a         1249 c         1231 g         1099 t
ORIGIN      
        1 gccattatga tatctgccag ttgccacatc agcggaaggc ttagcaaggt gccgccgatg
       61 accgttgcga aggtgcagat ccgcaaacac cagatcgctt taggggtgtt caggcgtaaa
      121 aagaagagat tgttttcggc ataaatgtag ttggcaacga tggagctgaa ggcaaacaga
      181 ataaccacga gggtaacaaa ctcagcaccc caggaaccca ttagcacccg catcgccttc
      241 tggataagct gaataccttc cagcggcatg taggttgtgt cgttacccgc cagtaatatc
      301 agcatggcgc ttgccgtaca gatgaccagg gtgtcgataa aaatgccaat catctggaca
      361 atcccttgcg ctgccggatg cggaggccag gacgccgctg ccgctgccgc gtttggcgtc
      421 gaacccattc ccgcctcatt ggaaaacata ctgcgctgaa aaccgttagt aatcgcctgg
      481 cttaaggtat atcccgccgc gccgcctgcc gcttcctgcc agccaaaagc actctcaaaa
      541 atagaccaaa tgacgtgggg aagttgcccg atatttatta cgcaaatcac catgctggtc
      601 agcacccaga ttatcgccat caacgggaca aacccctgca tgagccgggc gacgccatga
      661 agaccgcgag tgattgccag cagagcaaag acagcgagaa taatgcctgt caccagcggg
      721 ggaaaatcaa aagaaaaact cagggctcgg gcaacggcgt tcgcctgaac tccgctgaaa
      781 attatgccgt aggcgatgag caaaaagacg gcgaacagaa cgcccatcca acgcatcccc
      841 agcccgcgcg ccatatacca tgccggtccg ccacgaaact gcccattgac gtcacgttct
      901 ttataaagtt gtgcaaggga acattcggca aacgaggtcg ccatgccgat aaacgcggca
      961 acccacatcc aaaagacggc tctaggtcca ccggtggtaa tagccagcgc aacgccggcc
     1021 aggttgccgc tacccacgcg cgccgcaaga ctggtacaca atgactgaaa tgaggttaaa
     1081 ccgcctggct gtggatgaat gctattttta agacttttgc caaactggcg gatgtagcga
     1141 aactgcacaa atccggtgcg aaaagtgaac caacaacctg cgccgaagag caggtaaatc
     1201 attaccgatc cccaaaggac gctgttaata aaggagaaaa aatctggcat gcatatccct
     1261 cttattgccg gtcgcgatga ctttcctgtg taaacgttac caattgttta agaagtatat
     1321 acgctacgag gtacttgata acttctgcgt agcatacatg aggttttgta taaaaatggc
     1381 gggcgatatc aacgcagtgt cagaaatccg aaacagtctc gcctggcgat aaccgtcttg
     1441 tcggcggttg cgctgacgtt gcgtcgtgat atcatcaggg cagaccggtt acatccccct
     1501 aacaagctgt ttaaagagaa atactatcat gacggacaaa ttgacctccc ttcgtcagta
     1561 caccaccgta gtggccgaca ctggggacat cgcggcaatg aagctgtatc aaccgcagga
     1621 tgccacaacc aacccttctc tcattcttaa cgcagcgcag attccggaat accgtaagtt
     1681 gattgatgat gctgtcgcct gggcgaaaca gcagagcaac gatcgcgcgc agcagatcgt
     1741 ggacgcgacc gacaaactgg cagtaaatat tggtctggaa atcctgaaac tggttccggg
     1801 tcgtatctca actgaagttg atgcgcgtct ttcctatgac accgaagcgt caattgcgaa
     1861 agcaaaacgc ctgatcaaac tctacaacga tgctggtatt agcaacgatc gtattctgat
     1921 caaactggct tctacctggc agggtatccg tgctgcagaa cagctggaaa aagaaggcat
     1981 caactgtaac ctgaccctgc tgttctcctt cgctcaggct cgtgcttgtg cggaagcagg
     2041 cgtgttcctg atctcgccat ttgttggccg tattcttgac tggtacaaag cgaatactga
     2101 taagaaagag tacgctccgg cagaagatcc gggcgtggtt tctgtatctg aaatctacca
     2161 gtactacaaa gagcacggtt atgaaaccgt ggttatgggc gcaagcttcc gtaacatcgg
     2221 cgaaattctg gaactggcag gctgcgaccg tcttaccatc gcacctacac tgctgaaaga
     2281 gctggctgag agcgaagggg ctatcgaacg taaactgtct tacaccggcg aagtgaaagc
     2341 gcgtccggcg cgtatcactg agtccgagtt cctgtggcag cacaaccagg atccaatggc
     2401 agtagataaa ctggcggaag gtatccgtaa gtttgctatt gaccaggaaa aactggaaaa
     2461 aatgatcggc gatctgctgt aatcattctt agcgtgaccg ggaagtcggt cacgctacct
     2521 cttctgaagc ctgtctgtca ctcccttcgc agtgtatcat tctgtttaac gagactgttt
     2581 aaacggaaaa atcttgatga atactttacg tattggctta gtttccatct ctgatcgcgc
     2641 atccagcggc gtttatcagg ataaaggcat ccctgcgctg gaagaatggc tgacatcggt
     2701 gctaaccacg ccgtttgaac tggaaacccg cttaatcccc gatgagcagg cgatcatcga
     2761 gcaaacgttg tgtgagctag tggatgaaat gagttgccat ctggtgctca ccacgggcgg
     2821 aactggcccg gcgcgtcgtg acgtaacgcc cgatgcgacg ctggcagtag cggaccgcga
     2881 gatgcctggc tttggtgaac agatgcgcca gataagcctg cattttgtac caactgcgat
     2941 cctttcgcgt caggttggtg tgattcgcaa acaggcgctg atccttaact tacccggtca
     3001 gccgaagtct attaaagaga cgctggaagg tgtgaaggac gctgcgggta acgttgtggt
     3061 acacggtatt tttgccagcg taccgtactg cattcagttg ctggaagggc catacgttga
     3121 aacggcacca gaagtggttg ctgcattcag accgaagagt gcaagacgcg acgttagcga
     3181 ataaaaaaat ccccccgagc ggggggatct caaaacaatt agtgggattc accaatcggc
     3241 agaacggtgc gaccaaactg ctcgttcagt acttcaccca tcgccagata gattgcactg
     3301 gcaccacaga tcagaccaat ccagccggca aagtggatga ttgcggcgtt accggcaatg
     3361 ttaccgatcg ccagcagggc aaacagcaca gtcaggctaa agaaaacgaa ttgcagaacg
     3421 cgtgcgcctt tcagcgtgcc gaagaacata aacagcgtaa atacgcccca cagacccagg
     3481 tagacaccaa ggaactgtgc atttggcgca tcggtcagac ccagtttcgg catcagcaga
     3541 atcgcaacca gcgtcagcca gaaagaaccg taagaggtga atgcggttaa accgaaagtg
     3601 ttgccttttt tgtactccag cagaccagca aaaatttgcg cgatgccgct gtagaaaatg
     3661 cccatggcaa gaataatacc gtccagagcg aaataaccca cgttgtgcag gttaagcaga
     3721 atggtggtca tgccgaagcc catcaggccc agcggtgccg gattagccaa cttagtgttg
     3781 cccataattc ctcaaaaatc atcatcgaat gaatggtgaa ataatttccc tgaataactg
     3841 tagtgttttc agggcgcggc ataataatca gccagtgggg cagtgtctac gatcttttga
     3901 ggggaaaatg aaaattttcc ccggtttccg gtatcagacc tgagtggcgc taaccatccg
     3961 gcgcaggcag gcgatttgca gtacggctgg aatcgtcacg cgataggcgc tgccgctgac
     4021 cgctttaacc ccatttagtg ccgcacctac agggcctccc agccccgcgc cgcgcagcaa
     4081 accatgccca agtacgctca ttgctgcgtg ggtgcgtaaa atgcgggtca gttggctgga
     4141 aagcaaatgc gacacgcctt ttgccaataa tttgtctttc atcagcagcg gcagcagctc
     4201 ttccagctca ttcaccctcg catcgaacgc gtgcagaaac tcctgcttat gttcctcgtc
     4261 cattttcttc caggtattac gcagaaattg ttccagtaac tgttgctcaa tttcaaacgt
     4321 agacatctct ttgtcggctt tcagcttcaa tcgttttgaa acatcgagca aaatggcccg
     4381 atacaattta ccgtgtccgc gcagtttgtt ggcgatacta tcgccaccaa aatgctgtaa
     4441 ttctccagca atcagctgcc agttgcggcg atgttgctcg ggatgccctt ccatcgattt
     4501 aaacagttcg ttgcgcatca gtacgctgga gaggcgagtt ttgccttttt cattatgggt
     4561 gagcaatcgg gcgaaatttg ccaactgttc ctcactacaa tgctgaagaa aatccagatc
     4621 tgaatcattc aggtaattaa cattcatttt ttgtggcttc tatattctgg cgttagtcgt
     4681 cgccgataat tttcagcgtg gccatatccg atgagttcac cgtatgaccg gaaaaggtga
     4741 tttttgagac gcagcgttta ttgtcgttat cactgttaat gttgatccag tcagtggttt
     4801 gcccttcttt tatttctgaa ggaatattca gactctgact ggcgctacgg gcgg
//
LOCUS       DAJTSL010000222         4761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTSL010000222 DAJTSL010000000
VERSION     DAJTSL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.225"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1075
                     /locus_tag="OO433_003856"
     CDS             <1..1075
                     /locus_tag="OO433_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR6052158.1"
                     /translation="VEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETA
                     RLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPV
                     SLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELG
                     QQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPE
                     LFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQA
                     YPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYE
                     GVAMEYAFLVALAQGTLPATPAD"
     gene            complement(1072..1734)
                     /gene="exoX"
                     /locus_tag="OO433_003857"
     CDS             complement(1072..1734)
                     /gene="exoX"
                     /locus_tag="OO433_003857"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6052159.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWSFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(1758..2414)
                     /locus_tag="OO433_003858"
     CDS             complement(1758..2414)
                     /locus_tag="OO433_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR6052160.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLACDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(2516..2746)
                     /gene="holE"
                     /locus_tag="OO433_003859"
     CDS             complement(2516..2746)
                     /gene="holE"
                     /locus_tag="OO433_003859"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6052161.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVKLSRLPYEPKLK"
     gene            2885..3259
                     /gene="yobA"
                     /locus_tag="OO433_003860"
     CDS             2885..3259
                     /gene="yobA"
                     /locus_tag="OO433_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6052162.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3263..4135
                     /gene="copD"
                     /locus_tag="OO433_003861"
                     /pseudo
     CDS             3263..4135
                     /gene="copD"
                     /locus_tag="OO433_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            4148..4489
                     /locus_tag="OO433_003862"
     CDS             4148..4489
                     /locus_tag="OO433_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6052163.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
BASE COUNT         1180 a         1135 c         1219 g         1227 t
ORIGIN      
        1 ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa attaaccgca agacccggga
       61 agtcattggt attgcctttg atgatccagc ctatgtgacc tggattgcct acaatccaga
      121 acctgaaacc gcgcgattgc gttatggtta ttcttccatg accacaccag acactttgtt
      181 tgaactggat atggataccg gtgagcgtcg tgtattaaaa caaacggaag ttcctggttt
      241 tgatgcggcg aattaccgca gtgaacacct gtggatagtc gcccgtgatg gcgtcgaagt
      301 tccggtttcg ttggtctacc atcgcaaaca ttttcgcaaa ggacacaacc cgttgctggt
      361 gtatggctat ggttcttacg gcgcaagtat tgatgccgat ttcagtttta gccgcttgag
      421 tttgttagat cgtggctttg tctacgccat tgtccatgtt cgcggcggtg gtgagctggg
      481 gcaacaatgg tacgaagacg gaaaatttct gaagaagaaa aatacgttta atgattatct
      541 tgatgcctgc gatgcattgt taaaactggg ctatggctct ccttcgcttt gttatgcgat
      601 gggcgggagt gcggggggca tgttgatggg cgttgcaatt aatcaacgcc cggaattatt
      661 ccacggcgtt atcgcccagg taccgtttgt tgatgttgta acaacgatgc ttgatgaatc
      721 aattcctctt accactggtg agtttgaaga gtggggtaac ccgcaggatc cgcaatatta
      781 cgagtacatg aaaagctaca gcccgtatga caacgtcacc gcacaggctt atccgcattt
      841 actggtaacg accggtttac acgattctca ggtgcaatat tgggaaccgg caaaatgggt
      901 cgctaaattg cgcgagctga aaaccgataa ccatctttta ttgctctgta ccgacatgga
      961 ctcaggccat ggcggtaaat ctggccgctt taaatcgtac gaaggcgtag cgatggaata
     1021 tgcttttctg gtcgcgctgg cgcagggaac attacccgct acgcctgcgg attaagtatt
     1081 ttccagataa tgtttcagtg ttaaacgcag ctccgggctc atgctgtcca ggttattaaa
     1141 tgaccagcgc agatagcccg gatcgcgttc ggcaacgtcg gaaaccgctt tgccacggta
     1201 tttgccaaag gtgaaggtcg tcatcaacga cggacgtccg gtgatatcgg ccatctgttc
     1261 tgccgtccag ccggaggtgt tcataatatc gataagcaac gcggcggtga tataacagtc
     1321 atacaacgcg cggtgatgat gcaggcccgg cggcgtctgt acattgagct tccgtgtttt
     1381 atataacgcc atattgctgt acttgatccc tggccacaaa cgacgggcca gtttcatagt
     1441 gcaaatccac tcaccgggca tctcaggcag tacgcggcgg tcaaagctgg cgttgtgcgc
     1501 gacataccat tcactaccgt aatagtgtgg gatcacatct tcaatccacg gtttatcggc
     1561 gaccatggct tcggtgatgc gatgaatcgc catcgcttgt ggactaatag gacgatcggg
     1621 gcgcaccagg tggctcatgg ggttgacgat ttttccgtca atgacatcaa cagaggcaat
     1681 ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg ataatgcgca acatggcatg
     1741 ctccaggccg aaaaagccta gcgtaatgga atgatatcgc cttgccaacc ctgtgatgaa
     1801 cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa cgatgagacg accatattca
     1861 tcccatagtg cactgttacc acgggcattt gccatcagca cggcaatcga gtactgatgt
     1921 gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg gttctcccag acattgccct
     1981 gtagtaaaca gtgaacaggt cgggtccata tccatacctt gcggttgttc atcgaccacc
     2041 gtgatggttc tggaacgacg gcccagacat gcaccatgac tctgatggta gatcccaggc
     2101 gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa agcgatcgtt atattcaacg
     2161 ggaaggccgg caatgatggt cattcgccag gtcgttgcgg cataacaaag cgggtctaac
     2221 agtgagagat cgggtggggc aggaagggca cgtcgcgaat aatcacacgc cagtaaagaa
     2281 agcgatggaa aaactaacag ctgacattgt tgcctggcgg cggccctcac aaactccaga
     2341 tgatgagcta cctgttcggt gagtgaggtt ttacgaggct catattgcgc tgctgcaact
     2401 ttccagaacg acataatgac ttccttttca aaatgactgc aaattatggg tgcggtttag
     2461 aaaatactca taatttagca ggcgttatgt aagaaagtgt aactctgtat aagttttatt
     2521 taagtttggg ctcgtaaggt aaacgtgaca gtttgaccga agccaaacgg tgggcaataa
     2581 gcctctcgcg aaaccagctg cgcaaatgtt caggctgttc acgttcaacc gcttcagcga
     2641 tcaccggcat attgtagcgt tctttaaatg ccaccccggc cgccgccaaa tcgacattca
     2701 ctttatccat ttctgtttga tccagtttag ccagattctt cagcattctt aatctcctca
     2761 tcattcgcgg gcaaaagtta ctaagagtga gccaggatgg caagccggaa ttgatgatcc
     2821 tcgactcaga cattagacaa ggttatgctc tgtaaaaaag ttattacaaa aagtaataga
     2881 taatatggct tcaactgcac gctcccttcg ctacgcgctg gcaatcctga ccacttcgtt
     2941 ggtgacacct tccgtctggg cacatgcgca tttaacgcat cagtatcctg cggcaaacgc
     3001 gcaagtgaca gctgcaccgc aggcaatcac tttaaacttc tcggaaggtg ttgaaacagg
     3061 attcagtggt gcaaaaatca cggggccaaa aaacgaaaat attaaaacat taccggcgaa
     3121 gcgaaatgag caggaccaaa agcaactgat tgttccactg gccgattcgc tgaaacccgg
     3181 aacctacacc gttgactggc atgttgtttc ggtggacggg cataaaacga aaggacacta
     3241 cacctttagc gtgaaataaa tcatgctggc gtttacctag atcgcgctgc gatttatcca
     3301 tttcacctcg ctgatgctgg tttttggctt cgcaatgtac ggtgcctggc tggccccctt
     3361 gacgattcgt cgcttgctgg cgaagcgttt tctgcgttta caacagcacg ctgccgtctg
     3421 gagtttgatc agtgccactg caatgctcgc cgttcagggc gggttgatgg gaacgggatg
     3481 gacagatgta ttttcgccaa acatttggca ggctgttttg caaacgcagt ttggtggcgt
     3541 ctggttatgg caaattgttc tcgccctcgt aacgttgatc gtcgccctta tgcaaccgcg
     3601 gaatatgcca cgtttgctgt ttatgctcac taccgcgcaa tttattctgc tggcgggagt
     3661 ggggcatgcg acgctgaatg aaggggtaac ggcgaaaatc catcagacta atcacacgat
     3721 tcacctgatt tgtgcggcgg cgtggtttgg gggattactg ccggtgctct ggtgtatgca
     3781 actcatcaaa ggccgttggc gacaccaggc tattcaggcg ctgatgcgtt tttcatggtg
     3841 cgggcatttt gcggtgatcg gcgtactggc aagcggcgtg cttagtgcat tgttgattac
     3901 cggatttccc cccacgctta cgacttactg gggccaactc ttattgctca aagccatcct
     3961 ggtgatgatt atggtggtca ttgcgctggc taatcgttat gttcttgtac cgcgcatgag
     4021 gcaggatgaa gatcgtgcgg caccctggtt tgtgtggatg actaaactcg aatgggcgat
     4081 aggtgcggta gtgctggtga tcatcagcct gcttgcgact ctcgaacctt tttgatggat
     4141 tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta ctcaccagtt ctgcagcggc
     4201 gctggctgca ccgcaggtga ttaccgtcag tcgttttgaa gtgggtaaag acaaatgggc
     4261 gtttaatcgc gaagaggtga tgctgacttg ccgaccgggt aatgctttgt atgttattaa
     4321 tccgagtacc ctcgtgcagt atcctttaaa cgatatcgca caaaaggaag ttgccagtgg
     4381 gaagaccaat gcccaaccca tttcggtgat tcagattgat gatcctaaca atcccggcga
     4441 aaaaatgagt ctggcaccgt ttatagaacg agctgaaaaa ctctgttaat tacctaaaat
     4501 agccttttga tttccaataa aaaaaccgcc tcagttcttt caccagaacg ggcggtttta
     4561 acatttcagc tgatgaccac cacgcttttt attgaccatt ttgcacgcaa actggaaaac
     4621 ctggcgtcgt catctattct taaagggcaa ggcaactaag cctgcattaa tgccaacttt
     4681 tagcgcacgg ctctctccca agagccattt ccctggaccg aatacaggaa tcgtattcgg
     4741 tcttttttta attgcatttg a
//
LOCUS       DAJTSL010000223         4737 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTSL010000223 DAJTSL010000000
VERSION     DAJTSL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.226"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..495)
                     /gene="hyfE"
                     /locus_tag="OO433_003863"
     CDS             complement(<1..495)
                     /gene="hyfE"
                     /locus_tag="OO433_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6052164.1"
                     /translation="MIGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLV"
     gene            complement(507..1614)
                     /locus_tag="OO433_003864"
                     /pseudo
     CDS             complement(507..1614)
                     /locus_tag="OO433_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(1626..1736)
                     /locus_tag="OO433_003865"
     CDS             complement(1626..1736)
                     /locus_tag="OO433_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR6052165.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(1753..2700)
                     /gene="hyfC"
                     /locus_tag="OO433_003866"
     CDS             complement(1753..2700)
                     /gene="hyfC"
                     /locus_tag="OO433_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6052166.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(2711..4728)
                     /gene="hyfB"
                     /locus_tag="OO433_003867"
                     /pseudo
     CDS             complement(2711..4728)
                     /gene="hyfB"
                     /locus_tag="OO433_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
BASE COUNT         1312 a         1337 c         1248 g          840 t
ORIGIN      
        1 caccagatgg gagccgtttt ccatcaggca gtaaccaaaa atttgccgca ggatattgcg
       61 ctggctgaca atgcacagca ggccaagcag aaaatgacct aacgctaccg ccagcgccgg
      121 tttcagcccg gtagccatcg gtagcttcac gggctgaacg acaaatgcgc aaagcaggac
      181 aattaacgcc gcgagcagtg ccatcattgc cggaccgaat aacgcttttt ccgggatgtt
      241 ctggggaata tttcgtgcag cgtaagtcat gattaacggt accagcagca ctttggtgat
      301 aaaggcgctg gcggaccaga tcagcagttg ctctgcggcg aacaggcacg agagagtggc
      361 gaaaatagac accagcacca gcgactggca ggcgtaaaat ccgcatgaca ggcgatagct
      421 tttgacgcta atcacaaaca gcgatgtcag catcatcagt cccgccagat tatttacgat
      481 catagaaccg atcattcatc atctccttac tgcaaccagg tcgcggcgat tacgctggaa
      541 atcagcgaca tcacaatcag tacaatcaac accaggcgca ttgaccctgg cagcggtgcg
      601 gcatcggcga cggcctcgct cggtttgcca ggcacaacgc gaccaaacca gcgaataaac
      661 caggcgaaac tggcgaccga ttcaatcatc agcagaatca tggcgggcag caggatccag
      721 tactccactg acaacgcaaa accggcggca aacagcggga atttactgaa gaagccgttg
      781 aacggcggca caccggtaat cgccagcgct gccacgcaga aacccacgcc tggcaacggc
      841 agggagtgca atacgccacg cagacgcggc aacaagcgcg tgccgcagct gtaactcagc
      901 gcacctgcta caaggaaaaa caggctttta gcgaacgcgt ggttgacgat gtaggcgatg
      961 ctgccctcca gtgccagccg cccactttca ccatcgatgc ggcgtggaga taggctctga
     1021 tcggtgttgg cgcttccatt gcgtccggta gccacgcttg catcggtagc tgggccgatt
     1081 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccatgtaact
     1141 cgctcatcgc gctaagcaca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     1201 acaaccccaa cgaaccgata tgggtgataa gcaacgcttt tagtgttgaa cgctgcgctt
     1261 tatcgctctg gtaataactg atcaacgccc aggagcagcc gccctaattt caaaaaacaa
     1321 caactgaccg agcagcgtcg aggagagtac cagtcccgcc atcgcgccga taaacaccag
     1381 caaaaatgcg taataacgat tcgtgccgtt atgcgggtgt tcgcgatttt tatccgtcag
     1441 ataacccgtg gagtagatcg tgaccagcaa accgagaaac accaccacaa acagaatcag
     1501 cgtactcacg cgatcaatga ccaggccaaa caatgccaca tcccccacgt tgaccaacgt
     1561 caacgtgacg gcaactttat cggcctgata aaacgcggag atcagcgaca acatgcacag
     1621 cgtggtcagt gcggcgaaca aaacccccat tcggcggccc gacgttgtgg cgaaaacgaa
     1681 acgaccagtg cgccgataaa aggcagcatt aacgtcgtca gagcaagatt ttccatattc
     1741 cgtcagtgct ccttacagac cggttaacca gaagacccat gcaagcgcag caaggctgaa
     1801 gccaagccag gtcacatggt gaatgagtaa aaaacgcccg cgtgccagcg tgttttctgc
     1861 gattgaggcc agtacaaaaa tcagcaaaac cttgagcagc gtaacgacaa gtgaagtcag
     1921 caggcaggcg agagaaagtt cttgcgcgcg cccaaagggc agaaacaggg ccacaaacag
     1981 tgctgccatc acgacctgtt tcagccccag cccccatttc gctagcgcca gcccggcacc
     2041 ggaatattcg gtcagcgggc cttcctgtaa ttcctgttct gcttcagcaa catcaaaggg
     2101 aatttttccc atctcaatga agcaggcaaa accacaggcc agtaacgcca gtacggtggt
     2161 tagtggcgag ttccagcccg ttgccagcgt attgctgatc atctcgatat gcgtggaacc
     2221 tgctatcagc gccaatacca gcagtgagag aatgagcatg ggttcgacca gaatgccgag
     2281 cgtcaactcg cgactggcac cgactcccgc aaacgggctt ccggtatcca gtccggaaag
     2341 agcaaagaaa aaacgaaaca gggcaagaag atagataagg gtgatcagat cgccgccgcc
     2401 cgcaaaaggg gaaacggtaa taaaaagtgg tagggccatc gccagcacca gcatgctgct
     2461 gattaatacc cacggcatca ggcggaacat cagacctgaa gatataggcg caacttcctg
     2521 gcgtttaaac agtttgtgga tctcgcgata atcctgccag atccccggcc cgcggcggga
     2581 gtgcatacgc gcgcgtatct gccgggaaat acccgtaaaa agcggggtta gcagcagcag
     2641 aatcacggcc tgtgctaacg caaaaatgac cagatatccg tcgcaaagag tttgtctcat
     2701 ggtgatttcc ttagacggca atagcgatta gcagcacaac cagtgcggca actagataca
     2761 ggcaatagag acgaaagtcg ccgctttgca gatgctgtat ttctttggcc agccgttggg
     2821 tggcgctcac gatggggcgg atcacccgct catcccagaa gggttctgtg ctctgagccc
     2881 tggcggtgac gtgcgcaaga tctttattta gcctcagcgt agggtcgagc tgttgtcgta
     2941 gacgaaataa cgcagagaag accacacgca gcggctgcat cacgccattg cctgacggag
     3001 ccatcgcgtt ttcccagccg tagccgcatg cccaggcatc tcctgtgcga cgaaacgctg
     3061 cgcggcgcga acgacaccac agccagtaaa ggccaggcag caaaggcatc gccagtagta
     3121 acagcaacag taatgaagga gtgacccgtg tatgaaacgt gccgggtaca agtgctattc
     3181 cgctggcgac agtgacggga ggggtattgg taaacgcatg ggcaatatgc attatcttcg
     3241 gtgccagcca actggcacta agcgcaatta atacgcagag tgccgcgagc agtagcatgg
     3301 cgacgatcat cgtatttggc acttcctgtg cctcttcagc gtgtgtactg cgcggcgcac
     3361 cacagaaagt aataccgtac attttgacga agcacattac tgccagccca ccagtgactg
     3421 ccagcattac catagcaata ggacccgcaa gttgtagcgc tacggcttcc acacgactta
     3481 gtgagaacag cgactgccag gtgtaccatt cgctgataaa accattcagc ggaggaatgg
     3541 ctgatatcgc gaggcaacca atcaggcatg ctgcggctgt ccacggcatc cgtttcgcca
     3601 gtgcccccat tttttccatg tcgtgggtat gcaaacgcga aataatcgcg cccgcgccga
     3661 gaaatagcag ccctttgaac agcgcatggt tgagcagatg aaacagtgcg ccgagcagtc
     3721 caaccacggt gagcagcggg tcgtgcagtg acagaccgtc catcgccaca ccgactgcca
     3781 gcaaaataat gccgacgttt tcgacggtac tccaggccag cagccgtttg atatcctgtt
     3841 ccgccagcgc atatagcacg cccaggagcg cggagattgc gccgatcgcc atcaccagaa
     3901 tgccccacca cagaggcaaa cccgtttgcg ccagcagatc catcgctact ttcaggatgc
     3961 cgaaaatacc gattttgacc attacgccag acatcaacgc cgaagcgtgc gatggtgcgg
     4021 cagggtgagc gcgcggcaac cagctgtgca acggcatcat cccggctttc gcgccaaaac
     4081 cgaaaaaggc cagcagaaac atcgccgacg ccagccccgg agaaagtgaa agcgtgcgaa
     4141 actggcaaaa tcgaggctgc cgctttcgcg ccccatcagc aagaaggcga tcataatcag
     4201 caccgatccg gcgtgggcga taaaaaagta gagcatgcca gcgttgatcg acgttttatc
     4261 ctgcctggca atgaccagaa accaggaaga cagcgacatc atttcaaaca gcacgatgaa
     4321 ccaaaaagcg ttgtccatca ccagcagggc aaccatcgat gcgatgaaaa gattcataaa
     4381 gaagcccatc gccgccgcgc ctttgccctc gtattcgcgc atataagtca atgaatagag
     4441 cgaacaaacc accaccagca aggagataac cagcaccata aataccgaga ggctatccat
     4501 tcgcagggtt aaatcggcaa agtcaaaagg ggtggcaaaa cgggcgacta aagttacacc
     4561 agcatgtaat tgcgtaattc cgctgatgat gccaatcacg ccgcccacca gcgatgtgat
     4621 gccggaaagc ttaatagcca gtctgtccag accgagtaaa aacagcgaag ccagactggc
     4681 aaacagaaag aggatcagcg accaggttaa taattgcagg gcatccatta gtgttgc
//
LOCUS       DAJTSL010000224         4675 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTSL010000224 DAJTSL010000000
VERSION     DAJTSL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.227"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            60..1859
                     /gene="cysJ"
                     /locus_tag="OO433_003868"
     CDS             60..1859
                     /gene="cysJ"
                     /locus_tag="OO433_003868"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6052167.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDIEAADEFLSELRVERRYQRDVY"
     gene            1859..3571
                     /gene="cysI"
                     /locus_tag="OO433_003869"
     CDS             1859..3571
                     /gene="cysI"
                     /locus_tag="OO433_003869"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6052168.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPHHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            3646..4380
                     /gene="cysH"
                     /locus_tag="OO433_003870"
     CDS             3646..4380
                     /gene="cysH"
                     /locus_tag="OO433_003870"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6052169.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
BASE COUNT         1153 a         1219 c         1335 g          968 t
ORIGIN      
        1 tgttttatgg gcgctgacag ggcgcagaaa cagctttgct tactggaaca taacgacgca
       61 tgacgacaca ggtcccacct tccgcgttgc ttccgttgaa cccggagcaa ctggcacgcc
      121 ttcaggcggc cacgaccgat ttaacgccta cccagcttgc ctgggtttct ggctatttct
      181 ggggcgtact caatcagcag cctgctgcgc ttgcagcgac gccagcgcca gccgcagaaa
      241 tgccgggtat aactattatc tccgcctcgc aaaccggcaa tgcgcgccgg gttgctgaag
      301 cattacgcga tgatttatta gcagcaaaac tgaacgttaa gctggtgaac gcgggcgact
      361 ataaattcaa acaaatcgcc agcgaaaaac tgctcatcgt agtgacgtca acgcaagggg
      421 aaggggaacc gccggaagaa gccgtcgcgc tgcataagtt cctgttctcc aaaaaagcgc
      481 caaagctgga aaacaccgcg tttgccgtgt ttagcctcgg cgatagctct tatgaatttt
      541 tctgccagtc cgggaaagat ttcgacagca agctggcgga actgggtggt gaacgcctgc
      601 tcgaccgtgt cgatgccgat gttgaatacc aggctgctgc cagcgagtgg cgcgcccgcg
      661 tggttgatgc gcttaaatcg cgtgcgcctg tcgcggcacc ttcgcaatcc gtcgctactg
      721 gcgcggtaaa tgaaatccac accagcccgt acagcaaaga cgcgccgctg gtggctagcc
      781 tctctgttaa ccagaaaatt accgggcgta actctgaaaa agacgttcgc catatcgaaa
      841 ttgacttagg tgactcgggc ctgcgttacc agccgggtga cgcgctgggc gtctggtatc
      901 agaacgatcc ggcactggtg aaagaacttg tcgaactgct gtggctgaaa ggcgatgaac
      961 ctgtcaccgt cgagggcaaa acgttgcctc tgaacgaagc gctacagtgg cacttcgaac
     1021 tgaccgtcaa caccgccaac attgttgaga attacgccac gcttacccgc agcgaaacac
     1081 tgctgccgct ggtgggcgat aaagcgaagt tacagcatta cgccgcgacg acgccgattg
     1141 tcgacatggt gcgtttctct ccggcgcaac tggatgccga agcgctgatt aatctgctgc
     1201 gcccgctgac cccgcgcctg tattccatcg cctcctcgca ggcggaagtc gagaacgaag
     1261 tacacgtcac cgttggtgtg gtgcgttacg acgtggaagg ccgagcccgt gccggtggtg
     1321 cctccagctt cctcgcggat cgcgtggaag aagagggcga agtccgcgta tttatcgaac
     1381 ataacgataa ctttcgcctg cccgctaacc cggaaacccc ggtgattatg attggcccag
     1441 gcaccggtat tgcgccgttc cgcgccttta tgcagcaacg cgccgccgac gaagcgccag
     1501 gtaaaaactg gctgttcttt ggtaatccgc actttacgga agacttcctg taccaggtgg
     1561 agtggcagcg ctacgtcaaa gatggcgtgc tgacacgtat cgatcttgcc tggtcgcgcg
     1621 accaaaaaga aaaagtttac gtacaagaca aactgcgcga acagggcgcg gagctgtggc
     1681 gctggatcaa tgatggtgcc cacatttatg tctgcggcga cgctaatcgc atggcgaaag
     1741 acgttgagca ggcacttctg gaagtgattg ccgaatttgg tggcatggac atcgaagcgg
     1801 cggatgaatt tttaagtgag ctgcgcgtag agcgccgtta tcagcgagat gtctactaat
     1861 gagcgaaaaa catccagggc ctttagtggt cgaaggaaaa ctgacagacg ccgagcgcat
     1921 gaagcttgaa agcaactacc tgcgcggcac cattgcggaa gatttaaacg acggtctgac
     1981 cggcggcttt aagggcgaca acttcctgct gatccgcttc cacggcatgt atcagcagga
     2041 tgaccgcgac atccgcgccg aacgtgctga acagaagctg gagccgcacc acgcgatgct
     2101 gcttcgctgt cgtctgccgg gtggggtgat taccactaaa cagtggcagg cgatcgacaa
     2161 atttgccggt gaaaacacca tctatggcag cattcgcctg accaaccgcc agacattcca
     2221 gttccacggc attctgaaaa agaacgtcaa accggtgcac cagatgctgc actcggtcgg
     2281 tcttgatgcg ctggcgacag ctaacgacat gaaccgtaac gtactctgca cctcgaaccc
     2341 ttacgagtcg cagctgcacg cggaagcgta cgagtgggcg aagaagattt ctgagcatct
     2401 gttgcctcgt acccgcgcgt atgcggagat ctggctcgac caggaaaaag tcgccactac
     2461 tgatgaagaa ccgatccttg gtcagaccta cctgccgcgt aagttcaaaa ccacggtagt
     2521 gatcccgccg cagaacgata tcgatctgca tgccaacgac atgaacttcg tggcaatcgc
     2581 cgaaaacggc aagctggtag gcttcaacct gctggtgggc ggtgggcttt ctatcgaaca
     2641 cggcaacaag aaaacctacg cccgcacggc gagcgagttt ggctatctgc cgctggagca
     2701 tacgctggcg gtggccgaag ctgtcgtgac aactcagcgt gactggggta accgaaccga
     2761 tcgtaaaaat gccaaaacca aatacacgct ggagcgcgtg ggggttgaga cgtttaaagc
     2821 ggaagtggag cgtcgcgcgg ggatcaaatt tgaaccgatc cgtccatatg agttcaccgg
     2881 acgaggcgat cgtattggct gggttaaggg cattgatgat aactggcacc tgacgctgtt
     2941 tatcgaaaat ggtcgcatcc ttgattatcc ggggcgtccg ctgaaaaccg gcctgctgga
     3001 gatcgcgaag atccacaaag gcgatttccg cattacggcg aaccagaatc tgatcatcgc
     3061 cggtgtaccg gaaagcgaga aagcgaagat cgagaagatc gccaaagaga gcgggttaat
     3121 gaatgccgtc acgccgcagc gtgaaaactc gatggcctgc gtgtcattcc cgacttgccc
     3181 gctggcgatg gcggaagcag agcgtttcct gccgtctttt atcgacaaca tcgataattt
     3241 aatggcgaaa catggtgtca gcgatgagca tatcgtgatg cgtgtaacag gctgcccgaa
     3301 cggttgtggt cgcgcgatgc tggcggaagt gggcctggtg ggtaaagcgc cgggtcgcta
     3361 caacctgcat cttggcggca accgcattgg gacacgtatc ccacggatgt ataaagaaaa
     3421 catcaccgag ccggaaatcc tggcgtcgct tgatgaactg atagggcgct gggcgaaaga
     3481 gcgcgaagcg ggtgaaggct tcggcgactt tacggtgcgt gcgggcatca ttcgcccggt
     3541 gctcgatccg gcgcgcgatt tgtgggatta accatcagcc cggtcttgta ggcctgataa
     3601 gaacgcgtga gcgtcgcatc aggcaaggca aacagtgagg aatctatgtc caaactcgat
     3661 ctaaacgccc tgaacgaact gccgaaggta gatcgcattc tggcgctggc ggaaactaac
     3721 gccgaactgg aaaaactgga cgctgaaggc cgcgtagcct gggcgctgga taatctgccc
     3781 ggtgaatatg tactttcttc cagctttggc attcaggcgg cggtgagcct gcatctggtg
     3841 aatcaaattc gcccggatat tccggtgatc ctcaccgata cgggttactt gttcccggaa
     3901 acctaccgct ttattgacga gttaacggac aaactcaagc tcaacctgaa agtgtaccgt
     3961 gctaccgaaa gcgcagcctg gcaggaagca cgctacggaa aactgtggga gcagggcgtt
     4021 gaaggcattg aaaagtacaa tgacatcaac aaagtcgaac cgatgaaccg ggctctgaaa
     4081 gaacttaacg cgcaaacctg gtttgctggc ctgcgccgtg aacaatccgg cagtcgcgcc
     4141 aatttaccgg tgctggcaat tcagcgtggc gtatttaaag tgctgccgat tatcgactgg
     4201 gataaccgaa ctatttatca gtacctgcaa aaacatggcc tgaaatatca cccattatgg
     4261 gatgaaggat atttatcggt cggtgatacc catacaaccc gtaaatggga acctggcatg
     4321 tcggaagaag aaacacgttt ctttggctta aaaagggaat gtggattgca cgaagggtaa
     4381 atattgccgg ataaattaaa cgcctctgtc agaaatgatg ggggcgtttt tattttgcga
     4441 gcagtattcc tgattttaaa cgtgaacgcc gctggaagaa ttatcaggta tagaatggcg
     4501 tcgggtgctt gaggctgtct ggcgttaagc attcgcgagg ttccagatgg acaaaagccc
     4561 caggcgatat ttctatcaac ctgaggccag cgttcgaacc caaacaattc gaatgttagt
     4621 ctcttctttg cagggaggca agacatgctg acaaaatatg cccttgtggc aatcg
//
LOCUS       DAJTSL010000225         4652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTSL010000225 DAJTSL010000000
VERSION     DAJTSL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.228"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..755)
                     /locus_tag="OO433_003871"
     CDS             complement(<1..755)
                     /locus_tag="OO433_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR6052170.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLQQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLL"
     gene            complement(886..2250)
                     /gene="sdaA"
                     /locus_tag="OO433_003872"
     CDS             complement(886..2250)
                     /gene="sdaA"
                     /locus_tag="OO433_003872"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6052171.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(2434..3012)
                     /locus_tag="OO433_003873"
     CDS             complement(2434..3012)
                     /locus_tag="OO433_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6052172.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVIGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(3016..4377)
                     /gene="pabB"
                     /locus_tag="OO433_003874"
     CDS             complement(3016..4377)
                     /gene="pabB"
                     /locus_tag="OO433_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6052173.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVTKILRQ
                     LEK"
     gene            4451..4630
                     /locus_tag="OO433_003875"
     CDS             4451..4630
                     /locus_tag="OO433_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6052174.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
BASE COUNT         1167 a         1199 c         1208 g         1078 t
ORIGIN      
        1 cttagcagca ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc
       61 cccggaccat taacgttaat ggtaaaagga aagcgttgcg aagaaacctg atagttttca
      121 ttgtcttcag gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg
      181 tccaccgtca ggctggcgct actgatttgc tgcagttgtg gctcaaggag cattgccgtc
      241 agtaagtcga tgttaatcat ctccatgacc ccagcattgc tactgcccgc tgcaggcgtc
      301 cattgaatca aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt
      361 ggttgcggtg caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa
      421 atgctggagc aatagagtgt gccgtcttgc accaggccaa tggatcgcac agtttggagt
      481 tttgccgcct gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga
      541 agcaagactt cgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg
      601 tgattggcga attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg
      661 ctcagggtcg aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgt
      721 cgataggttt taatgatccg ttgtgctttt tgcatgcggg ccaccctgta agcgtatggc
      781 gacaaacgcc tgacgcaaca gtggatgtgt agtggggaat attacagcag acgagaaagc
      841 gggtataaat tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac
      901 tttgattgcc agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccggt
      961 ttcgtacatc gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag
     1021 agccatccgt gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg
     1081 cacctgaacc tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat
     1141 ttccgccgcc acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc
     1201 agccattgaa caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga
     1261 ggcgttcatt ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat
     1321 gtccgggctg accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc
     1381 gcaggcaccg ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac
     1441 tgccagcgca aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc
     1501 gctggaaacc agcatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg
     1561 cagtacacct tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg
     1621 cgcgaaatac tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag
     1681 accagacagc gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa
     1741 cggatacggc acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat
     1801 aaaaccgccg ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc
     1861 gtggatttgc ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt
     1921 gtcgcgcggg aaatccactt catgccgtcc ctgtgccagc agcagacgtt cgcgctcttc
     1981 tacgtcgcga ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc
     2041 aagacccata ataatggcaa tatcggtgtg gtggccttta cccgtcagcg acagtgaacc
     2101 ataaacgtcc acggcaacgc gagtaacgct atccagtaag cctttttcga ccagatcatc
     2161 gacgaactgt ttacccgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc
     2221 caccttaaac atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt
     2281 ccagtaacga actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta
     2341 ttcacatgaa ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca
     2401 agtagtgttt tgtagatgta aagataagta tagtcagggt ttcacaccaa tttgcagcgc
     2461 cagctcacga attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag
     2521 ccatacccga tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg
     2581 taatgcctgg gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata
     2641 cggcagatcg ggcgggataa tgccgatcac tggggttacc tgatagccag ttacgctatc
     2701 gacgggcggc agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc
     2761 agcttcgcgc agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg
     2821 gaatgccact tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc
     2881 cggttgcggt cgacggacga tggggattaa cacggcagcc tgacgatgat ttagggtttc
     2941 ccggttaatt tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa gcgtcaggct
     3001 acggtattcc acgtcttact tctccagttg gcgtaatatc ttagtaactt tatcaaaagt
     3061 ttcctgatat tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta
     3121 tatttgtccg ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca tgttgccgca
     3181 aaagctcaaa tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc
     3241 gataatttcc atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc
     3301 gcgcagcaga tcgctggcgt gtaactgttc tggtagtcgc gccgttatag tgctgaccag
     3361 atgatgcacg gcagggaagg gttccaccac gaagagctct ggtactttta ccgaaccggc
     3421 tacggcaaca cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg
     3481 atctttcgct gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag
     3541 gcgtggtagc gtgcctttaa tcgggcgggt ctggatttca ctattatcac aaagaataaa
     3601 ccgctctggc gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg
     3661 cgcgcggttg gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc
     3721 atgaaaacgc tgggcgagat tcacctgata gcaatcaccg ctgtgcagat attcctgtac
     3781 ctggcgaaat ttttcgccgt actgctcgcg ggtcatattg gattgccagt cggaagtgag
     3841 cgtgaaatct tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac
     3901 atcattatga ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc
     3961 gtagataccc actgccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga
     4021 ctcaaaacgg cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa
     4081 atcttcgtta tgcgttgggc gaatgtctgc gcgatccagc acctgctgga gcacctgtag
     4141 cgggtcatca gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttaccgaa
     4201 agtggttaaa gtgcaaatcg gctcggcgac cacaatatca aagcggctat acggatgatc
     4261 ggcatagccg gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa
     4321 ttcagcggcg tcctgacgcc agggtaaagt aatcacagcg ggagataacg tcttcatcag
     4381 tcctgactct actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca
     4441 ggagttaaca atgtttgcag gtttaccttc actcacccat gaacagcagc aaaaagctgt
     4501 cgagcggatc caggaactga tggcacaagg gatgagcagc ggccaggcaa tcgcgctggt
     4561 ggcagaagag ctgcgtgcca accactccgg cgagcgaatc gtggcgcgtt ttgaggatga
     4621 agacgagtaa cacactaatc cgtatcgatt tc
//
LOCUS       DAJTSL010000226         4587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTSL010000226 DAJTSL010000000
VERSION     DAJTSL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.229"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            254..616
                     /gene="queD"
                     /locus_tag="OO433_003876"
     CDS             254..616
                     /gene="queD"
                     /locus_tag="OO433_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6052175.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            694..1962
                     /locus_tag="OO433_003877"
     CDS             694..1962
                     /locus_tag="OO433_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6052176.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWQLVGDALRSCVNTGISVRGMGMALTGAQAAQTLISACQHREP
                     QNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKPV
                     PPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            1953..2213
                     /locus_tag="OO433_003878"
     CDS             1953..2213
                     /locus_tag="OO433_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6052177.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQWLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFSITYRFG"
     gene            2230..2805
                     /locus_tag="OO433_003879"
     CDS             2230..2805
                     /locus_tag="OO433_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR6052178.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDACNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(2953..3813)
                     /locus_tag="OO433_003880"
     CDS             complement(2953..3813)
                     /locus_tag="OO433_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6052179.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGIQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(3810..>4587)
                     /locus_tag="OO433_003881"
     CDS             complement(3810..>4587)
                     /locus_tag="OO433_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6052180.1"
                     /translation="NILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADEQ
                     AAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPEF
                     VARHIAEWQHQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWTCFTQVERFTLDALFIT
                     LEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPAM
                     QCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
BASE COUNT         1055 a         1235 c         1165 g         1132 t
ORIGIN      
        1 aagataaggt taacggggtg aacggtgtgg attaaagacg ggatagcgat aactaataac
       61 caaatcgact aacctgtttt agcaatagtc tttaacaaca aaatagatta accaacccaa
      121 tgaaaaacaa atgaatttag ccaatcatta agataaatca gcgattttgc gcaacaaagt
      181 cgttttagat aatgcgaaaa aacagccttt ccggtactct acggcggttt tatcgtcctg
      241 tagagaaatt atgatgtcca ccacgttatt taaagatttc accttcgaag ccgctcaccg
      301 cttaccacac gtcccggaag ggcataaatg tggtcgcctg cacgggcatt cctttatggt
      361 gcgactggaa attaccgggg aagtcgatcc gcatacgggc tggattatcg atttcgctga
      421 actaaaagcg gcgtttaagc caacttatga gcgcctcgat caccattatc tcaatgatat
      481 tccaggtctg gaaaacccaa ctagcgaagt tttagcaaaa tggatttggg atcaggttaa
      541 acccgttgtg ccgctgttaa gtgcggtgat ggtaaaagaa acctgcaccg ctggttgtat
      601 ctatcgcggc gaatgataag agtgtgtcgg cggtcaattt cccttaagta acgctatgtt
      661 agggtattgt gttcaggata tctggggcat gacatggaag acgactgcga cattattatt
      721 attggtgccg gtattgcagg caccgcttgc gcgttacgct gcgcgcgagc gggtttatcc
      781 gttttgttac tggaacgcgc tgaaatcccc ggcagcaaaa atctttccgg cggacggtta
      841 tatacccatg cactcgcgga actcctccct cagtttcatc tgaccgcgcc tcttgaacga
      901 cgcatcactc acgaaagcct ttccctgtta acgcccgatg gcgtaacgac gttttccagc
      961 ttacagcccg gcggtgaatc ctggagtgta ttacgtgcac ggttcgatcc gtggctggtt
     1021 gccgaagccg aaaaagaagg tgtcgaatgt atccccggag cgacggtgga tgcactgtat
     1081 gaagaaaacg gcagagtctg tggcgttatt tgtggtgacg atattctccg cgcccgttat
     1141 gtggtgctgg cagaaggtgc caacagcgtc ctggctgaac gtcacgggtt agtgactcgt
     1201 cctgctggcg aagcgatggc gttggggatc aaagaagtgc tgtcgctgga aacatccgct
     1261 attgaagaac gttttcatct ggagaataac gaaggcgcag cgttgctgtt cagcggcgga
     1321 atctgtgatg acttacccgg cggcgcattt ctttatacta atcaacaaac gctctcgtta
     1381 gggattgttt gcccgctctc ttcccttacg caaagtcgtg ttccggcaag cgagctgctg
     1441 actcgcttta aagcgcatcc ggcagtgcgc ccgcttatca aaaacacgga atcactggag
     1501 tatggtgcgc atctggtgcc agaaggtggc ttgcacagta tgccggtgca atacgccggt
     1561 aacggctggc agctggtggg cgatgcgttg cgcagttgcg tcaataccgg aatttccgtg
     1621 cgcggcatgg gtatggcgct gactggcgcg caggcggcac aaacgctgat aagcgcctgc
     1681 cagcaccgcg agccgcaaaa tctgtttgcg ctttatcatc acaacgtaga gcgcagcctg
     1741 ctatgggatg ttctacaacg ttatcagcat gttccggcgc ttttgcaacg ccctggctgg
     1801 tatcgggcgt ggcctgcgtt aatgcaggat atttcccgcg atttatggga tcagggtgat
     1861 aaacctgttc caccgctgcg ccagttattc tggcgtcatt tacgtcgtca tggcctgtgg
     1921 catctggcgg gcgatgttat caggagtctg cgatgtctgt agcccataat ctctggcgcg
     1981 ttgctgatgc gccgcacatt gttccggctg actccgttga gcgtcagatg gtacaatggt
     2041 tgattaacgc ctgtccggca ggtctttttt cgctcacacc ggaaggtgac ttacgcgttg
     2101 attatcacgg ctgcctggag tgtggcacct gccgtttgct gtgcgacgaa tccacactac
     2161 aacagtggcg ctatccgcct tccggattca gcatcaccta ccgctttgga taaataataa
     2221 ggataattta tgcccctctt acacctgctc cgccagaatc cggtgattgc tgccgttaaa
     2281 gataatgcca gcctgcaact ggcaatcgac tctgaatgcc agtttatttc cgtgttgtac
     2341 ggcaatatct gcaccatcag taatatcgtt aaaaagatta agaacgccgg gaaatatgct
     2401 tttattcatg ttgatttgct ggaaggcgcg tcaaataaag aagtcgtgat tcagtttttg
     2461 aaactggtga ccgaggcgga cggcattatc agtaccaaag cctcaatgtt gaaagcggca
     2521 agagcggaag gttttttctg tattcatcgc ctgtttattg ttgattcaat ttcgtttcac
     2581 aacattgata agcaagttgc gcaatcgaat ccggattgta ttgagatcct gccaggctgt
     2641 atgcccaaag tgctgggctg ggtgacagag aaaatccgcc aaccgctgat tgccggtggg
     2701 ctggtgtgcg atgaagaaga tgcgtgtaat gcgattaacg cgggtgtcgt ggcgctttcc
     2761 accacgaata ccggggtctg gacgttagcg aaaaaattac tttgacggga taatcgtaac
     2821 caattgaatt tggtttgatt tgtaggccgc acgccacatc cgacattcag cgcctgatgc
     2881 gacgcttgac gcgtcttatc aggcctacaa gtccccaggc cagaaccgta ggcaggatca
     2941 tccggcaaca cattattggc aatctgcgtg aatgtttgtc accagtgcct ccagcaccac
     3001 tttccagtca tcaaccacgc caacatctgc ctgtgaaaat accgcagcgc tggcgtcatg
     3061 attaatcgcc accacaaact tgctattgcg tacgcccgcc attaacgctg ctgcgccgga
     3121 agcgccaaca acaatacaca cctcaggtgc cagcaaatgt ccggaaatac caatcacttt
     3181 ttcagcatca acgccaccgt tcatcactcg cgcccgactg tagcccactt cggctcccag
     3241 cttttctgcc agcatagcga tttcctggtt atctgcctct ccgccctgcc ccaccaccag
     3301 cacccgtctg gcctcagcta atgggtcgcg agtaacattt ttcagatcct ctgtgctaac
     3361 cagccagtcg ggaagcgcac ccgggacaat gttcagttgt tgtattccag agggcaacgt
     3421 ggcgtttttc gctgctcccg cctgacgcgc cagagaaagg catagcgggc gtttttctgt
     3481 ttgcagcgtc gccgttagcg catttcccca gtgggatttg cgcacgctta cggttggaat
     3541 atctaacgaa gtcacctggc agatgcttgc accatgtaaa cgccaggcca gccgggtcga
     3601 cagttcatcg ccaaacgttc ccggcggaaa gagaacaacg tccgcaggcg ttcgctgcca
     3661 ctgttccacc aacgcatcca gcacctgttc cgccactaca ggctgaggtt caatctgcca
     3721 gtgcgccagg gtacaaccac tgaaatcctg cgccgccagc cagcttgcta ttgcggcatt
     3781 ctcctgattt attgtcacaa ttgcgatatt catggctgca tcctttggcg taaatagtcc
     3841 tgccatagct tttgagcttt ttctgctacc gtttgcccat caatcagcgt cgctccgcgt
     3901 cgttgctctg ctcttgccag ttgcagacac tgcatcgctg gcatttcggc agcaacagtt
     3961 ttacggataa tttctgcttt ccccgcagcc attcgctggc gcattcccgg tactggcaag
     4021 gccacttcac cgcactgacg cacggcaatc accgcaggca agcgaacccg gcaacaacgc
     4081 aacccatgtt cagtacgctg ttcgagggtg ataaacagcg cgtcgagagt gaaacgctcc
     4141 acctgggtga agcaggtcca gcccagcatt tccgccagca aaaatggcgt ttgcccattc
     4201 tgcccttcgc tgctttggct gccggtaatt atcagatcca gcggattctg atgctgccat
     4261 tcggcaatat ggcgagcgac aaattccggc gcaaagcgca gatctgccgc cgtctccagc
     4321 aatacagctt cctcaaaccc aagagccatg agatagcgca gccagtgcaa tgcccgttca
     4381 tcccccatgc tcaacgcggt taaagacatc ggcgtaccgt tttttctctg cgccaacagc
     4441 agcgcggcag cggcctgttc atcagcaccg agtaaacttc gcagtagcga aatatccggt
     4501 ccgcttttac cctgagccgc cgcctgccac tctttttccg ccagcattcc ggcatccggt
     4561 tcggctttaa acgctaacag aatgttc
//
LOCUS       DAJTSL010000227         4578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTSL010000227 DAJTSL010000000
VERSION     DAJTSL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.230"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..893
                     /gene="tauA"
                     /locus_tag="OO433_003882"
     CDS             <1..893
                     /gene="tauA"
                     /locus_tag="OO433_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6052181.1"
                     /translation="VTVAYQTSAEPAKVAQAGNTFAKESGASVDWRKFDSGASIVRAL
                     ASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTISKPEDLIGKRIA
                     VPFISTTHYSLLAALKHWGIKLGQVEIVNLQPPAIIAAWQRGDIDGAYVWAPAVNALE
                     KDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAIDAQQPYIANPDAW
                     LKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNKVIIDTAQFLKEQ
                     GKVPAVANDYSQYVTSRFVQ"
     gene            906..1673
                     /gene="tauB"
                     /locus_tag="OO433_003883"
     CDS             906..1673
                     /gene="tauB"
                     /locus_tag="OO433_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6052182.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQVLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1670..2479
                     /gene="tauC"
                     /locus_tag="OO433_003884"
     CDS             1670..2479
                     /gene="tauC"
                     /locus_tag="OO433_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6052183.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVSPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTP"
     gene            2494..3345
                     /gene="tauD"
                     /locus_tag="OO433_003885"
     CDS             2494..3345
                     /gene="tauD"
                     /locus_tag="OO433_003885"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6052184.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGITAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRVTILGD
                     KPFYRAG"
     gene            complement(3451..4425)
                     /gene="hemB"
                     /locus_tag="OO433_003886"
     CDS             complement(3451..4425)
                     /gene="hemB"
                     /locus_tag="OO433_003886"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6052185.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1013 a         1184 c         1355 g         1026 t
ORIGIN      
        1 acgtcaccgt ggcgtatcaa acctcagccg aaccggcgaa agtggctcag gccggcaaca
       61 cctttgctaa agagagcggg gcgagcgtgg actggcgtaa gtttgacagc ggagccagca
      121 tcgtgcgggc gctggcttct ggcgacgtgc aaatcggcaa cctcggttcc agcccattag
      181 cggttgcagc cagccaacag gtgccgattg aagtcttctt gctggcgtca aaactgggta
      241 actccgaagc gctggtggta aagaaaacta tcagcaaacc cgaagatttg attggcaaac
      301 gcattgccgt accgtttatc tccaccaccc actacagcct gctggcggcg ctgaaacact
      361 gggggattaa actagggcaa gtggagattg tgaacctgca gccgcccgcg attatcgccg
      421 catggcagcg gggtgatatt gatggtgctt atgtctgggc accggcggtt aacgccctgg
      481 aaaaagacgg caaggtgttg accgattctg aacaggtcgg gcagtggggt gcgccaacgc
      541 tggacgtctg ggtggtgcgc aaagattttg ccgagaaaca tcctgaggtc gtgaaagcgt
      601 tcgctaaaag cgccatcgat gctcagcaac cgtacattgc taacccagac gcgtggctga
      661 aacagccgga aaacatcagc aaactggcgc gtttaagcgg cgtgccggaa ggtgacgttc
      721 cggggctggt taaggggaat acctatctga cgccgcagca acaaacggca gaactgaccg
      781 gaccggtgaa caaagtaatc atcgacaccg cgcagttttt gaaagagcag ggcaaggtcc
      841 cggctgtagc gaatgattac agccagtacg ttacctcgcg cttcgtgcaa taaaaggagg
      901 cgcagatgct gcaaatctct catctctatg ccgattatgg cggcaaaccg gcactggaag
      961 atatcaacct gacgctggaa agcggcgagc tactggtggt gctggggccg tccggctgcg
     1021 gtaaaaccac gttgctgaat ctgattgccg gttttgtgcc ttatcagcat ggcagcattc
     1081 aactggcggg taagcgtatt gagggaccgg gagcagagcg cggggtggtt tttcagaatg
     1141 aagggctgct accgtggcgc aatgtacagg acaacgtggc gttcggcctg caattggcag
     1201 gtatagagaa aatgcagcga ctggaaatcg cgcaccaggt gctgaaaaaa gtggggctgg
     1261 aaggcgcaga aaaacgctat atctggcagc tttccggtgg tcaacgtcag cgggtgggga
     1321 ttgcccgcgc gctggcggcg aatccccagc tgttattact cgacgaaccg tttggtgcgc
     1381 tggacgcctt cacccgcgac cagatgcaaa ctctgctgct gaaactctgg caggagacgg
     1441 gcaagcaggt gctgttgatt acccacgata tagaagaagc ggtgtttatg gcgactgaac
     1501 tggttctgct ttcatccggc cctggccgtg tgctggagcg gctgccgctc aactttgctc
     1561 gccgctttgt tgcgggagag tcgagccgca gcatcaagtc cgatccacaa ttcatcgcca
     1621 tgcgcgaata tgttttaagc cgcgtatttg agcaacggga ggcgttctca tgagtgtgct
     1681 cattaatgaa aaactgcatt cgcggcggct gaaatggcgc tggccgctct cgcgtcaggt
     1741 gaccttaagc attggcacgt tagcggtttt actcactgta tggtggacgg tggcggcgct
     1801 gcaactgatt agcccgctgt ttttgccgcc gccgcaacaa gtactggcaa aactgctcac
     1861 cattgccgga ccgcagggat ttatggacgc cacgctgtgg cagcatctgg cagccagcct
     1921 gacgcgcatt gtgctggcgc tactcgcggc ggtggtgatc ggtattccgg tcgggatcgc
     1981 catggggcta agcccgacgg tgcgcggcat tctggatccg gtaatcgaac tttatcgtcc
     2041 ggtgtcgccg ctggcttatt tgccgctgat ggtgatctgg tttggtattg gtgaaacctc
     2101 gaagatctta ctgatctatt tagcgatttt tgcaccggtg gcgatgtcgg cgctggcggg
     2161 ggtgaaaagc gcgcagcagg ttcgcattcg tgccgcccag tcgctgggtg ccagccgtgc
     2221 gcaggtgctg tggtttgtca ttttgcccgg cgcgctgccg gaaatcctca ccggattacg
     2281 cattggtctg ggtgtgggct ggtctacgct ggtagcggcg gagctgattg ccgcgacgcg
     2341 cggtttagga tttatggttc agtcagcggg tgaatttctc gcgactgacg tggtgctggc
     2401 gggaatcgcg gtgattgcga ttatcgcctt tcttttagaa ctgggtttgc gcgcgttaca
     2461 gcgccgtctg acgccctagc atggagaagt acaatgagtg aacgtctgag cattaccccg
     2521 ctggggccgt atatcggcgc acaaatttcg ggtgccgacc tgacgcgccc gttaagcgat
     2581 aatcagtttg aacagcttta ccatgcggtg ctgcgccatc aggtggtgtt tctgcgcgat
     2641 caagccatta cgccgcagca gcaacgcgcg ctggcccagc gttttggcga actgcatatt
     2701 caccctgttt accctcatgc cgaaggggtt gacgagatca tcgtgctgga tacccataac
     2761 gataatccgc cagataacga caactggcat accgatgtga catttattga aacgccaccc
     2821 gcaggggcga ttctggcagc taaagaatta ccttcgaccg gcggtgatac gctctggacc
     2881 agcggtatta cggcctatga ggcgctctct gttcccttcc gccagctgct gagcgggctg
     2941 cgtgcggagc atgatttccg taaatcgttc ccggaataca aataccgcaa aaccgaggag
     3001 gaacatcaac gctggcgcga ggcggtcgcg aaaaatcctc cgttgctaca tccggtggtg
     3061 cgaacgcatc cggtgagcgg taaacaggcg ctgtttgtga atgaaggctt tactacgcga
     3121 attgtcgatg tcagcgagaa agagagcgaa gccttgttag gctttttatt cgcccatatc
     3181 actaaaccgg agtttcaggt gcgttggcgc tggcaaccga atgatattgc gatttgggat
     3241 aaccgcgtga ctcagcacta tgccaatgcc gattacctgc cacagcggcg gattatgcat
     3301 cgggtgacga ttcttgggga taaaccgttt tatcgggcgg ggtgatacga gtgcggacgg
     3361 ttccctcgcc cccttgggga gagggttagg gtgagggggc gttcaccgta ctttcaacag
     3421 gttaactccc cctttctgag aggaaaaaaa ttaacgcaga atcttcttct cagccaaatc
     3481 cagcgcaaag tagctgaaaa tcagatccgc acccgcacgc ttaattgaac ctaagctttc
     3541 gagcacgact ttctcttcat ctatagcacc cgccagcgcg gcgaacttaa tcatcgcgta
     3601 ctcaccgctc acctgatacg cgccaatcgg caattcagta cgttcacgca gctcacgcac
     3661 gatgtcgagg tacgctccag caggtttaac catcaggcag tctgcgccct gggcttcatc
     3721 cagcagtgac tcacgaatcg cctcgcgacg gttcataggg ttcatctgat agcttttgcg
     3781 gtcgcctttt aatgcgcttc cggcagcttc acggaacggg ccataaaagg aagaggcgaa
     3841 cttggtcgaa tacgacataa tcgccgtatc tttaaagcct gcagcgtcca gcgcctgacg
     3901 aatcgcctgt acctggccgt ccatcgcggc ggaaggggcg atgaagtctg cgcctgcagc
     3961 agctgcaacc acggcttgct tgcctaaatt ttccagagtc gcgtcgttgt cgacgccatg
     4021 ctcgcacagc acaccgcagt gaccgtgaga agtgtattca cagaaacaag tgtctgacat
     4081 gacgatcatt tctggcacag tctgcttgca gatgcgcgac atacgcgcca ccagtccatc
     4141 ttcccgccag gcgtcgctgc cggtttcatc ggtatggtga gagatgccga aagtcatcac
     4201 ggaacgaata ccggcgttgg cgatgcgttc aatttcgcgt gccagatgtt tctctggaat
     4261 gcgcatcacg cctggcatgg cttcaacggc tttgtagtcg tcaatttctt cttcaacaaa
     4321 gatcggcaac accaggtcgt taaggctaag tgttgtctct tcaaacatag cgcgcagcgc
     4381 aggagatttg cgcaggcgac gagggcgttg gattaagtct gtcatggtct gcctgatgtt
     4441 tgtggaatta aggtgcacag tatacctgaa gcggggcagg agtgttttac gaaagttgtc
     4501 gtgatgttga caagaagaga atgaaagaga gaccaggaca tctttgtaat agaaatagca
     4561 ataagttcat attcactg
//
LOCUS       DAJTSL010000228         4512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTSL010000228 DAJTSL010000000
VERSION     DAJTSL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.231"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..247)
                     /locus_tag="OO433_003887"
     CDS             complement(<1..247)
                     /locus_tag="OO433_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HCR6052186.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAN"
     gene            505..1284
                     /gene="deoC"
                     /locus_tag="OO433_003888"
     CDS             505..1284
                     /gene="deoC"
                     /locus_tag="OO433_003888"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6052187.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQTKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            1411..2733
                     /gene="deoA"
                     /locus_tag="OO433_003889"
     CDS             1411..2733
                     /gene="deoA"
                     /locus_tag="OO433_003889"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6052188.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDARALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            2785..4008
                     /gene="deoB"
                     /locus_tag="OO433_003890"
     CDS             2785..4008
                     /gene="deoB"
                     /locus_tag="OO433_003890"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6052189.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            4088..>4512
                     /gene="deoD"
                     /locus_tag="OO433_003891"
     CDS             4088..>4512
                     /gene="deoD"
                     /locus_tag="OO433_003891"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6052190.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAA"
BASE COUNT         1079 a         1159 c         1254 g         1020 t
ORIGIN      
        1 cgttcgccag aaaacgggcg taatcgggta agacatagcg cggtttgtac ggcgcatgac
       61 cttcaaacat atcgcagatt acaccctcat ccagcgcgcg gcgggcttcg gcaggaagct
      121 gcggataagg cagattgttt tctgcttcca gagccagaaa atggcgcttc tgctccgggc
      181 taagcactgg gctggtgaca atttgctggc aacgttgttg cagtgcattt tcatgagaag
      241 tgggcatctt cttttccttt tatgccgaag gtgatgcgcc attgtaagaa gtttcgtgat
      301 gttcactttg atcctgatgc gtttgccacc actgacgcat tcatttgaaa gtgaattatt
      361 tgaaccagat cgcattacag tgatgcaaac ttgtaagtag atttccttaa ttgtgatgta
      421 tatcgaagtg tgttgcggag tagatgttag aatactaaca aactcgcaag gtgaatttta
      481 ttggcgacaa gccaggagaa tgacatgact gatctgaaag caagcagcct gcgtgcactg
      541 aaattgatgg acctgaccac cctgaatgac gacgacaccg acgagaaagt aattgctctg
      601 tgtcatcaga ccaaaactcc ggtcggcaat accgccgcta tctgtatcta tcctcgcttt
      661 atcccgattg ctcgcaaaac gctgaaagag cagggcaccc cggaaatccg aatcgctacg
      721 gtaaccaact tcccacacgg taacgatgac atcgaaatcg cgctggcaga aacccgtgcg
      781 gcaatcgcct acggtgctga tgaagttgac gttgtgttcc cgtaccgcgc gctgatggcg
      841 ggtaacgagc aggttggttt tgacctggtg aaagcctgta aagaggcttg cgcggcagcg
      901 aatgtactgc tgaaagtgat catcgaaacc ggcgaactga aagacgaagc gctgatccgt
      961 aaagcgtctg aaatctccat caaagcgggt gctgacttca tcaaaacctc taccggtaaa
     1021 gtggcagtga acgcgacgcc ggaaagcgcg cgcatcatga tggaagtgat ccgtgatatg
     1081 ggcgtagaaa aaaccgttgg tttcaaaccg gcgggcggcg tgcgtactgc ggaagatgcg
     1141 cagaaatatc tcgccatcgc agatgaactg ttcggtgctg actgggcaga tgcgcgtcac
     1201 taccgctttg gtgcttccag cctgctggca agcctgttga aagcgctggg ccacggtgac
     1261 ggtaagagcg ccagcagcta ctaagcaaga tgctttacgc ctgatgcgct gcgcttatca
     1321 ggcctacgag acgtatctac ccgtaggccg gataaggcgt agacgcatcc ggcaaaagcc
     1381 gcctcatact cttttcctcg ggaggttacc ttgtttctcg cacaagaaat tattcgtaaa
     1441 aaacgtgatg gtcatgcgct gagcgatgaa gaaattcgtt tctttatcaa cggcattcgc
     1501 gacaacacta tctccgaagg gcagattgcc gccctcgcga tgaccatttt cttccacgat
     1561 atgacaatgc ctgagcgtgt ctcgctgacc atggcgatgc gagattcagg aaccgttctc
     1621 gactggaaaa gcctgcatct gaatggcccg attgttgata aacattccac cggcggcgtc
     1681 ggcgatgtca cttcgctgat gttggggccg atggtcgcag cctgcggcgg ctatattccg
     1741 atgatctccg gtcgcggcct cggtcatact ggcggtacgc tcgacaaact ggaatccatc
     1801 cctggcttcg acattttccc ggatgacaac cgtttccgcg aaattattaa agacgtcggc
     1861 gtggcgatta tcggtcagac cagctcactg gctccggcgg ataaacgttt ctacgcgacc
     1921 cgtgatatta ccgcaaccgt agactccatc ccgctgatca ctgcctcgat cctggcgaag
     1981 aaactggcgg aaggtctgga tgcgctggtg atggacgtga aagtgggtag cggcgcgttt
     2041 atgccgacct acgaactctc tgaagccctt gccgaagcga ttgttggcgt ggctaacggc
     2101 gctggcgtgc gcaccaccgc gctgctcacc gacatgaatc aggtactggc ctccagtgca
     2161 ggtaacgcgg ttgaagttcg tgaagcggtg cagttcctga cgggtgaata tcgtaacccg
     2221 cgtctgtttg atgtcacgat ggcgctgtgc gtggagatgc tgatctccgg caaactggcg
     2281 aaagatgacg ccgaagcgcg cgcgaaattg caggcggtgc tggacaacgg taaagcggca
     2341 gaagtctttg gtcgtatggt agcggcacaa aaaggcccga ccgacttcgt tgagaactac
     2401 gcgaagtatc tgccgacagc gatgctgacg aaagcagtct atgctgatac cgaaggtttt
     2461 gtcagtgaaa tggatgcccg cgcgctgggg atggcagtgg ttgcaatggg cggcggtcgt
     2521 cgtcaggcat ctgacaccat cgattacagc gtcggcttta ctgatatggc gcgtctgggc
     2581 gaccaagtag acggtcagcg tccgctggcg gttatccacg cgaaagacga aaacagctgg
     2641 caggaagcgg cgaaagcggt gaaagcggca attaaacttg ccgataaagc accggaaagc
     2701 acaccaactg tctatcgccg tatcagcgaa taacggtata ctgatctgat catttaaatt
     2761 tgaagcactg agtacggaga acatatgaaa cgtgcattta ttatggtgct ggactcattc
     2821 ggcatcggcg ctacagaaga tgcagaacgc tttggtgacg tcggggctga caccctgggt
     2881 catatcgcag aagcttgtgc caaaggcgaa gctgataacg gtcgtaaagg cccgctcaat
     2941 ctgccaaatc tgacccgtct ggggctggcg aaagcacacg aaggttctac cggtttcatt
     3001 ccggcgggaa tggacggcaa cgctgaagtt atcggcgcgt acgcatgggc gcacgaaatg
     3061 tcatccggta aagatacccc gtctggtcac tgggaaatcg ccggtgtccc ggttctgttt
     3121 gagtggggat acttctccga tcacgaaaac agcttcccgc aagagctgct ggataaactg
     3181 gtcgaacgcg ctaatctgcc gggttacctc ggtaactgcc actcttccgg tacggtcatt
     3241 ctggatcagc tgggcgaaga gcacatgaaa accggcaagc cgattttcta tacctccgct
     3301 gactccgtgt tccagattgc ctgccacgaa gaaacctttg gcctggataa actctacgaa
     3361 ctgtgcgaaa tcgcccgtga agagctgacc aacggcggct acaacatcgg tcgtgttatc
     3421 gctcgtccgt ttatcggtga caaagccggt aacttccagc gtaccggtaa ccgtcacgac
     3481 ctggctgttg agccgccagc accgaccgtg ctgcagaaac tggttgatga aaaacacggt
     3541 caggtggttt ctgtcggtaa aattgcagac atctacgcca actgcggtat caccaagaaa
     3601 gtgaaagcaa ctggcctgga cgcgctgttt gacgccacca tcaaagagat gaaagaagcg
     3661 ggtgataaca ccatcgtctt caccaacttc gttgacttcg actcttcctg gggccaccgt
     3721 cgcgacgtcg ccggttatgc tgcgggtctg gagctgttcg accgccgtct gccggagctg
     3781 atgtctttgc tgcgcgatga cgacatcctg atcctcactg ctgaccacgg ttgtgatccg
     3841 acctggaccg gtactgacca cacgcgtgaa cacattccgg tactggttta cggcccgaaa
     3901 gtaaaaccgg gttcactggg tcaccgtgaa accttcgcgg atatcggcca gactctggca
     3961 aaatattttg gtacttctga tatggaatat ggcaaagcca tgttctgata atgggtgcgg
     4021 cttgatgccc tcaccccggc cctctcccac agggagaggg tgaaaacatg ttgataagga
     4081 taaaacaatg gctaccccac acattaatgc agaaatgggc gatttcgctg acgtagtttt
     4141 gatgccaggc gacccgctgc gtgcgaagta tattgctgaa actttccttg aagatgcccg
     4201 tgaagtgaac aacgttcgcg gtatgctggg cttcaccggt acttacaaag gccgcaaaat
     4261 ttccgtaatg ggtcacggta tgggtatccc gtcctgctcc atctacacca aagaactgat
     4321 caccgatttc ggcgtgaaga aaattatccg cgtgggttcc tgtggcgcag ttctgccgca
     4381 cgtaaaactg cgcgacgtcg ttatcggtat gggtgcctgc accgattcca aagttaaccg
     4441 catccgtttt aaagatcatg actttgccgc tatcgctgac tttgacatgg tgcgtaacgc
     4501 ggtagacgcg gc
//
LOCUS       DAJTSL010000229         4502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTSL010000229 DAJTSL010000000
VERSION     DAJTSL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.232"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..571)
                     /locus_tag="OO433_003892"
     CDS             complement(<1..571)
                     /locus_tag="OO433_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6052191.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWFNSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHW"
     gene            complement(564..2843)
                     /locus_tag="OO433_003893"
     CDS             complement(564..2843)
                     /locus_tag="OO433_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052192.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQIALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQRNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLANAVTGCYLTPYSEKHLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASIADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DANFINALPDLRAVMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(2854..3942)
                     /locus_tag="OO433_003894"
     CDS             complement(2854..3942)
                     /locus_tag="OO433_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR6052193.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1048 a         1192 c         1228 g         1034 t
ORIGIN      
        1 gccagtgttg caggttggcg cgcagagtac ttttgaaatc aaagtttcgc gccagtggaa
       61 taaatgagcg gcgacggcga tcgcggacac cagaaaaagc ctgacgaact tcctttgcca
      121 gtcgcgccat aatttcttca acaacctggc gcacaatcct gcgggcggca gccagtactt
      181 cggggttcat cagatgtttg gtgtgcaaaa cggcgcgtag caggctttcg gaaggctgca
      241 tacgttccag cacgtcgaga tttgtcacca catcttcaat gccgtagcgc agtacggcat
      301 cgctttccag ccgctcaatc acctgttgcg gaaacagcgt gtggatactg ttgaaccact
      361 caggagtggt gagatttgag ccacctaatc caccagagcg ttcaccacgc tggagccgtt
      421 caggatcgcg cccatacagc cactccagcg cgtgatctat ctgccgggcg ttgtcatcca
      481 gcccacaaag cgtcgtttct gccgcttcgc caagaattaa tcgccagcgt tgtagctcac
      541 gggtggtcag aagatcgttc agttcagaca tggaaaactc cccagtgttg cagcgatgcc
      601 agtgcctgct gttcgagttg ttgatgatgt gcaatggctt gcggtggaca atgcaacggc
      661 atttgcagtg ccgaaacggg aagttgcgcc agttgataat gctcaagcac ctgatgcgcc
      721 agcgtcccgc gttctcgtgg cggtagccag gccatcaccg cgcgtaaatc gggcagggca
      781 ttgataaaat tggcatcact cagttgattc aaatggctgc tgaaaccggc gataaatgca
      841 ggttgacagg ccagttgatg acgggctaat gccagcaaac cgtgcagcgc ctcgccggat
      901 tgtgccgggg ataactgcgc cagcatcgtc agcgccgctt cggcactggc gttgggatgt
      961 tccagacgca ttagtgcgcc aagagccgcg ccgcgatcca gcgccggggc atgaatcgcc
     1021 tgactgcgtc gctccagtaa ggctaccgcc gcagaaagcg aaacgccgga taaattaacg
     1081 ccgctatgta gatcgcgcag aatatggcaa agcgcttgcc agctatgcag gtgagcgtga
     1141 aactcctttt catccggtct gccgttagat tcacacagcc acagcgtgcg atcgatagcc
     1201 gcgcataacg tcgtttgtaa tatctgcgcg ccttgcatac cgctaatttc atccagccgc
     1261 cataaggcat atagcacttc cagcgcctgg cccatttcgg caaattgatt ttcctgggcg
     1321 attaataatg tgagttgctc cagtaattgt tgactgaagg acgccagacc cgctaacgcc
     1381 gcctggctaa gacatgtggt gatactgccg atgccacccg cgtccagcat atcggcttct
     1441 aatttatggc gtgcggcttc ctgaagtgtt gcaccaaagc atgcggcctc aatcaatgcc
     1501 gcatgctggc taagcgggcg ggttaatttc cagtgttctt caccgttgcc tgcaagtgtc
     1561 agtgtacttc cctgctggcg tacaatccca gggatctcca gtattgccag ccgatgtaac
     1621 acctgacttt gcgccagccc atcgggggta aagcgattta aggtaagctc cgccggaaga
     1681 gagattgcgg tacgttccag ttcgcaggtg acatcttttg gcagaggcgg ttgtggcgtg
     1741 gaaggggcaa gtttaccgaa tccgtcaccc gctaatgtgt ctatcaacgt tagcagaatc
     1801 ggatcggtat cgggatgaat aacgccgcga tagctccacg gcaacggtgc attcagggct
     1861 tctttaatca gcgagcctgc tatggcatcc agccagtcag tgcgtaacgg tagtgtatga
     1921 ccgcgcaact gtgccagtgc cattgcatgc agatgtgcgg cagccatatc cgcgatcgaa
     1981 gcaggcaagt tgtgctggcg caaacgggtg agaaccgttt ttagcagttg ttcaccggcc
     2041 tgctgtaagc cccactgcca gcaccagttt tgccagaccg gggcaggcat accggaaagg
     2101 tatcctgcca gcacatcaag gtgcttttca ctgtaggggg tgagataaca acctgtaacg
     2161 gcatttgcca gcgagggcaa ttctggcttg ttaatttcct gcggacattc gcgccacatt
     2221 tttgccagtg caggagcgtg ccagccaccg cagacgacta acacgtcgcc attatttcgc
     2281 tgcaccgccc atgcaatcca gcgagccata aaggcttcgc gctgacgatt gagtgcatcg
     2341 ccggggaaat cgccccgcag ttgggcaaaa tagtgcgcca gcgcagaggg taatgcaatt
     2401 tgctggcttt catcttcgaa caagtgatcc cacagggtgt cgctgttatc catgcgggtg
     2461 gcacgcagca gcaaggcctg gctttcctct tgcgtatcag gcgaatcgtc cacttcttca
     2521 ctctgcgccc agcaaggcaa atcgatgaag taagtttgtg cctgaatacg acgtgcggct
     2581 tgtagcgcct gccactccgg cgaaaattca gcaaatggcg tccaggcacc acgcccgggg
     2641 gctgcaccgt cctgatactg gcaataactg taaatagcta ccggaagctg gtgggctaaa
     2701 aacagttcgt ctacccggtc attaaaatca gccgggcctt caatcaatac gtatcgtggc
     2761 cgctggcttt cgattaacga tttcaccagc cgggcgcagg ccggactatg atgccggatg
     2821 ccgacaatta acggctcgct catgcatctt tcctcacggc aggcggtggc gggcttgata
     2881 ataagcctgc cagtgagctt ctttatgtgt ggcgactcgt tgttcgaagt aacggcgcag
     2941 acgagcgcga tcttcttcgt tatctttgac gatggttccg gcaatgcagt ccaccaggtc
     3001 ggctggctca cctgcgcgat tcgctaaaaa ccaggcgcgg acgcctacgg cgtgggccac
     3061 gttcacggct tcagcggtgg acataatcgc tgttagtgta tccattgacg ttttcttttc
     3121 gccgttggca cgcagatcgc ggaaggtgcg gaccagtaac tccagtaccg cgtcaggcac
     3181 tttatgcgga ataccgctat gagccagcaa acgcgccgaa gcactggcga ccagttccag
     3241 ctcctgggcg aaatccataa tcggaaagac agtttcaaaa tcgaagcgac gctttagcgc
     3301 agcactcatt tcattgacgc cgcgatcgcg ggtattggca gtggcaataa tattaaagcc
     3361 ttctcgcgca tagagctgac tggcttcacc agtgagttcc ggcaccgtca tcaccctgtc
     3421 agaaagcata cctaacagac agtcttgcac ttccagcggt gtacgcgtta tctcttcaaa
     3481 acggacgatt ttgccgtcgc gcattccctg gtaaagcggg gcagggacca gcgcttccgt
     3541 tgatggacca tgattgataa gcagggcgta gttccagccg tacttgattt gatcttcggt
     3601 agtagatgcg ccgccctgga tggttaaccc ggcatcgccg ctaatagcgg ttgccagtaa
     3661 ttcagacagg agagattttg cggtgccagg ttcccccacc aacatcaaac cgcgtccggt
     3721 ggcaagagtg actaacatac gttcgacagc ggagggacta atcaccactt tacggctaat
     3781 attttgcgcg ctatcgccaa ggataaaagc acgggccgca ggcaaactta actgccaacc
     3841 gggcgggcaa ggtgcgttat cattttgttt taattttgcc agttcatcgg cgtataacac
     3901 agcagcaggc ggacgctgca gatgattatt ctgcggtgac atatattcat ccctgaaatg
     3961 gattaatgaa ataaacgata ttttacgcgc aataaatgtt tggcgcatta gttgtttgag
     4021 cgaaaaatat ctggaaagca gatcacagta aataaaaaaa cctgctggtg aggcaggttc
     4081 ttttatttac tactttcagg cctaacacca cagcctgtgc aacatcgtgt tccaacgaac
     4141 gaattataac cacggctgga ccccgttgca attaagataa tgagatttat aatccagacc
     4201 gttgggacgt tgcgccggat cgaatctatt tcaagtcctg gctaccggac gggtggtgct
     4261 gcaggtggtg ccctgatccg gagtgcaaca tcgtggccca acatacgtaa accagtatcg
     4321 acaggcagtg taagggtaag gtgctggtgt tgcgctcctt aataaggaga gcggatggac
     4381 acgctgacac aaaagttaac cgtgctcatt gctgtactgg agctattagt ggctctgtta
     4441 cggttgattg atttgttgaa gtaacgggca acttgaaacg catctaaggg ggaatacagt
     4501 tt
//
LOCUS       DAJTSL010000230         4425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTSL010000230 DAJTSL010000000
VERSION     DAJTSL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.233"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1089
                     /gene="nadB"
                     /locus_tag="OO433_003895"
     CDS             <1..1089
                     /gene="nadB"
                     /locus_tag="OO433_003895"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCR6052194.1"
                     /translation="VVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPDISSG
                     DGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFMPD
                     FDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLGID
                     LTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLECL
                     VYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVGIV
                     RTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKESRG
                     LHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(1074..1811)
                     /gene="trmN"
                     /locus_tag="OO433_003896"
     CDS             complement(1074..1811)
                     /gene="trmN"
                     /locus_tag="OO433_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6052195.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECAIPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            1943..3277
                     /gene="srmB"
                     /locus_tag="OO433_003897"
     CDS             1943..3277
                     /gene="srmB"
                     /locus_tag="OO433_003897"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6052196.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIEELRPKTRTPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(3486..4367)
                     /locus_tag="OO433_003898"
     CDS             complement(3486..4367)
                     /locus_tag="OO433_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6052197.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCIEECFLPG"
BASE COUNT         1126 a         1207 c         1121 g          971 t
ORIGIN      
        1 gttgtaggcg cgtgggtatg gaaccgaaat aaagaaacgg tggaaacctg ccacgcaaaa
       61 gcagtggtac tggcaaccgg cggtgcgtcg aaggtgtatc agtacaccac caatccggat
      121 atttcttctg gcgatggcat tgctatggcg tggcgcgcgg gctgtcgggt tgccaatctc
      181 gaatttaatc agttccaccc taccgcactg tatcacccac aggcacgcaa tttcctgtta
      241 acggaagcac tgcgcggcga aggcgcttat ctcaagcgcc cggatggcac gcgttttatg
      301 cccgattttg atgagcgcgg cgaactggcc ccgcgcgata ttgtcgcccg cgccattgac
      361 catgaaatga aacgcctcgg cgcagattgt atgttcctcg acatcagcca taagcccgcc
      421 gattttattc gccagcattt cccgatgatt tatgaaaaat tgctcgggct ggggattgat
      481 ctcacacaag aaccggtacc gattgtgcct gctgcacact atacctgcgg tggtgtaatg
      541 gttgatgatc atgggcgtac ggacgtcgag ggcttgtatg ccattggcga ggtgagttat
      601 accggcttac acggcgctaa ccgcatggcc tcgaattcat tgctggagtg tctggtctat
      661 ggctggtcgg cggcagaaga tatcaccaga cgtatgcctg atgcccacgg cgtcagtacg
      721 ttaccaccgt gggatgaaag ccgcgttgag aaccctgacg aacgggtagt aattcagcat
      781 aactggcacg agctacggct gtttatgtgg gattacgtcg gcattgtgcg cacaacaaaa
      841 cgtcttgagc gcgccctgcg gcgtatcaat atgttgcaac aggaaataga cgaatattac
      901 gcccacttcc gcgtgtcgaa taatttgctg gagctgcgta atctggtgca ggttgcggaa
      961 ttgattgttc gctgtgccat gatgcgcaag gagagtcgtg gcctgcattt cacactggat
     1021 tatccggaac tgctcaccca ttccggtccg tcgatccttt cccccggcaa tcattacata
     1081 aacagataaa aagcctgggt cagcgccgta tacgcttcgg aatagttctg gtctggccca
     1141 cgaatgacta agcgatcgct aaagcattct cccgcctgcg gggagaatgc cagcagcacc
     1201 cgatgcggca gtcgcgcttc gttttccgcc acatccgtcc gcaaacgtaa atgccagccc
     1261 atgcttaatg ccagctccgt aaaaccatta ccaatctgct ctggcagcac tacgcagaaa
     1321 aatccctctt cggtaatgca ctccgccgca caggtcagca gcgatgggtg atcaagcgta
     1381 gtggtatagc gagcctgttc ccgttgaggg atcgcgcact ctactccctg ctgatagtaa
     1441 ggtgggttac tgatgattaa atcgaagcgt actgtctgct gtgtgagcca ctgctgaata
     1501 tccgccgtat ggacgttaat ccgctctgcc cacggggact ggttgatatt ttcctgcgcc
     1561 tgcgcggcag cttcactttc cagctcaaca gcatcaatta tcacgctgtc actggttcgc
     1621 tgcgccagca tcagtgccag caacccgcta cccgcgccga tatcaaggca acgtttcacc
     1681 ccagccaccg gtgcccatgc gcccagcaaa atgccatccg ttcccacttt catcgcacag
     1741 cgatcgtgag caacaaaaaa ctgtttaaaa gtaaatccat tacgacgaag cacggatgta
     1801 gactgtgaca tgaaaataaa accttgcagg aaaaacggcg atagcaccgg gtgagaacaa
     1861 tacccgagaa gcgatatcca tacaaacaga tgaagattgc agccgtaacg tctataatca
     1921 gcgccccaca cagaggtaga acatgactgt aacgactttt tccgaacttg aactcgacga
     1981 aagcctgctg gaagccctcc aggataaagg tttcactcgc ccgaccgcca ttcaggctgc
     2041 cgccattccg cctgcgctcg atggccgtga tgtactcggt tctgcgccga caggcaccgg
     2101 taaaacggcg gcgtatctgc tgccagcgtt gcagcacctg ctcgatttcc cgcgtaagaa
     2161 atccggtccg ccgcgtattt tgatcctcac cccaactcgc gagctggcga tgcaggtggc
     2221 cgatcatgcc cgcgaactgg cgaaacatac gcatctggat atcgccacca ttaccggcgg
     2281 cgtagcctat atgaaccacg cggaagtgtt cagcgaaaat caggacatcg tggtcgccac
     2341 gaccggacgt ctgctgcaat acataaaaga agagaacttc gattgccgcg cggttgaaac
     2401 gctgatcctc gacgaagcag accgtatgct ggatatgggc ttcgctcagg atatcgaaca
     2461 tattgctggc gaaacgcgct ggcgtaaaca gaccctgctc ttttcggcaa cgctggaagg
     2521 cgatgcgatt caggactttg ccgagcgtct gctggaagat ccggtggagg tttctgccaa
     2581 tccgtccacc cgtgagcgca aaaaaattca tcagtggtat taccgcgccg atgacctgga
     2641 acataaaacc gcattgctgg tgcatctgtt aaaacagccg gaagcgaccc gctcaattgt
     2701 gtttgtgcgt aagcgtgaac gtgtgcatga gctggcaaac tggctgcgcg aagcgggcat
     2761 caacaactgc tatctcgaag gtgagatggt acagggcaag cgtaatgaag cgattaaacg
     2821 cctgaccgaa ggccgcgtaa acgtactggt agcaaccgat gttgccgcgc gcggtatcga
     2881 cattccagac gtcagccacg tctttaactt cgatatgccg cgcagtggtg atacttattt
     2941 gcaccgtatc ggacgtaccg cgcgcgccgg tcgtaaaggc accgcgattt cgctggtgga
     3001 agcccatgac catctgctgc ttggcaaagt tggccgttat attgaagagc cgataaaagc
     3061 tcgcgtcatt gaggaactac gcccgaaaac gcgtacgcca agcgagaagc agaccggcaa
     3121 gccatcgaag aaagtactgg caaaacgcgc cgagaagaaa aaagctaaag agaaagagaa
     3181 gccgcgggtg aaaaaacgcc atcgcgacac caaaaatatt ggtaagcgcc gtaaaccgag
     3241 cggaacgggc gtgccaccgc aaacgacaga agagtaatct taattccggg tttaagcccg
     3301 gaattaaaag tgctggcaaa cgcaaaactg cctgatgcgc tacgcttatc aggcctacat
     3361 ggctcatgca atatattgaa tttgcacgat cttgtaggcc ggataaggcg ttcacgccgc
     3421 atccggcatg aacaaaacgc acttcgtcga caagttgggt gccgggttta agcccggcat
     3481 tatttttatc ccggcaaaaa acactcttca atacactgaa taaaagtgtg catcgccggg
     3541 ctaaccgctt ttccagcatg gtgtgcgcac atagccgtaa tggtctgcga ctgttcgcca
     3601 aaaggcaatt caattaattc tccgctctca agctcttttt ccaccgcaaa gcggggcaag
     3661 taactgacac cgatattcgc cgcgacacaa cgtttgatac tttcaatgct taacagctca
     3721 atggtgtttt ccaccgtgat ccgccgctga cgcagcgtgc tctcaaatat ctgccggaag
     3781 acacattgcg gttcgttgat aataaagcta caggcgttat gtcttcccgg ctcagtaaaa
     3841 tcgacatctg caatttgcgg tgaagccacc agcaccagcg attgttcacc caactctcgt
     3901 cggttcaggg catcatcatt ccctacgcga taaaagacgc cgacatcggc ctcatcattc
     3961 agtagcgcat cacggatcac gtaacagttc agcgactgca acgataaacg cacttttggt
     4021 gcgcgttgcc gaaaacgctg caacacctgc ggcattcggt aagagagcaa cgtttcgccc
     4081 gaaacaacgc gtagttcccc gtccggatct gacccctttt tggcagcctc acgaagcgta
     4141 tccatcactc gggtaagttc ataaatgtgc ggcagcaact tttttccttc gcgggtaagg
     4201 cacatccgtc ggccaatttt ctcaaataac tggactgaaa actcctgctc aagctgctga
     4261 atatgaaaag tcaccgtcga ttgtgtacag cacaattttt gcgaagctcg caaaaaggaa
     4321 ccctcttcca ccacggtttt aagcgtaata aaacggcgca gatccataac cccgaaccta
     4381 tcgaaaatat cgaatctaga atataaaaac attcattttt ttaaa
//
LOCUS       DAJTSL010000231         4388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTSL010000231 DAJTSL010000000
VERSION     DAJTSL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.234"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(33..1247)
                     /gene="ydcO"
                     /locus_tag="OO433_003899"
     CDS             complement(33..1247)
                     /gene="ydcO"
                     /locus_tag="OO433_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCR6052198.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVARATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAEILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTVASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITEMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNQY"
     gene            1300..1836
                     /gene="sutR"
                     /locus_tag="OO433_003900"
     CDS             1300..1836
                     /gene="sutR"
                     /locus_tag="OO433_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCR6052199.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1909..3870
                     /gene="rlhA"
                     /locus_tag="OO433_003901"
     CDS             1909..3870
                     /gene="rlhA"
                     /locus_tag="OO433_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR6052200.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMNYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVAEPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3962..4192)
                     /locus_tag="OO433_003902"
     CDS             complement(3962..4192)
                     /locus_tag="OO433_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCR6052201.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1138 a         1129 c         1092 g         1029 t
ORIGIN      
        1 agggatccac gtctttcagg gcgtgtagga tgtcaatatt ggtttctgtc agcgattaaa
       61 ttcaacacca cgtaacaaac gcctccggca attaatcccc agaacgcaga accaatcccg
      121 accagcgtca atccacttgc cgttaccaga aatgccacca ccgccgcgtc gcgctcacgc
      181 tcattatgca gcgcctgata caaactgccg ccaatggtac tcaacagcgc cagacctgcc
      241 agcatctgga tccaacttac gggcagggca gccatcatct cggtaatggc actaccaaac
      301 agacctgcga gcaaatagaa aatgcctgca acggcagctg ccagccaacg ttgatcttta
      361 tccggatgcg cttccgggct ttggcaaata gccgcggtga ttgccgcaat accgacggaa
      421 taaacgccga aaggggaaaa aaccagtgcc agcaatccag taaatacaat taatggcgaa
      481 acaggagccg aatatccagc tgctttcatt gctgcgatac ccggtgcgtt ttgcgatgcc
      541 accgtcacca gaaaaagggg gagtgcaacg ctcaggctgt gagcaaacga aaaatcaggg
      601 gtaatataag tggggagaac gggtttaaag acaacatcag ttgtgacaac gtcaccttgc
      661 gcgatgacga tcacgatccc aataatcatc gcggcaatta ccgcatagcg cggcgcaacg
      721 gccttggttg ccagccatac cagcaacata cttccacaca acgtaaattg accgtccaga
      781 ctggcaaacg cctgtaaacc aaagcgtaat aaaatctcgg caagcattgc cgccgcaagc
      841 gagtgcggaa taatgcgcat cagacgagca aagagtcccg ttatgccgca gaggactatt
      901 aacgcgttgg tgacaataaa aacgccgatg gcttcgttaa gtgttagtcc ctgcaatccg
      961 gtgaccaaca aagccgcgcc aggcgttgac catgcggtga gaacaggtac gcgataccat
     1021 aatgtcagag tcagcgtact gacgcccatt gccagcccca gcgccgtcat ccagccagag
     1081 atttgtgcag tggtggctct ggcgacaatc gctgcttgcc agattattgc cgctgaactg
     1141 gcgtagccaa ttaataccgc cagaaacccc gccagtagcg tgggtggagg aatagaaaac
     1201 agacgcatag tcacctcgta cgttatagcg tacaggcacc gtagcatttg tccgttataa
     1261 cgcacaagtg ataaacttcc gttttgccgg aggagtcgca tggaaaatct cgctcgcttt
     1321 ttatccacca cacttaaaca actacgccag cagcgcggct ggagtctttc gcgactggca
     1381 gaagcgacgg gcgtttctaa agcaatgtta gggcagattg agcgtaatga gtccagtccg
     1441 acggtcgcga cattatggaa aattgccacc gggttgaacg taccattttc aacatttatt
     1501 tctccgccgc agtccgctac gccttcagtt tatgatccac aacagcaggc gatggtgatc
     1561 acttcgctgt ttccttatga tccacaactc tgctttgaac acttctcgat tcagatggca
     1621 tctggtgcaa taagcgaatc gacgccccat gagaaggggg taatcgaaca tgtcgtcgtc
     1681 attgatggac aacttgatct gtgcgttgat ggggaatggc agacccttaa ttgtggggaa
     1741 ggcgttcgat ttgctgcaga cgtcacgcat atctatcgta atggtgggga gcaaaccgta
     1801 cattttcatt ccctcatcca ttacccgcgc agttaagcgg gaaaactatt tcgcaacgtc
     1861 gcgcttgtgg ctaaaatagc cgccattttt cagctactgg ataagaatgt gaccgtatct
     1921 tctcatcgac ttgaactgtt aagcccggca cgcgatgccg ccattgcccg cgaagctatt
     1981 ttgcacggtg ccgatgctgt ttatatcggc ggccctggtt ttggtgcccg tcataatgcc
     2041 agtaatagcc tgaaagatat tgccgagctg gtgccgtttg cccatcgtta tggtgcaaaa
     2101 attttcgtca cgcttaacac cattttgcat gatgatgagc tggaacccgc gcaacggctg
     2161 attactgacc tctaccagac cggtgtcgat gcgctgattg ttcaggatat ggggattctg
     2221 gaacttgata ttccgccgat tgaactgcac gccagtacgc agtgcgacat tcgtacagtt
     2281 gaaaaagcga agttcctctc tgatgttggc ttcacgcaga ttgtgctggc gcgagagctg
     2341 aatcttgatc agatccgcgc gattcaccag gctacggacg cgaccattga attctttatt
     2401 catggggcac tgtgcgtggc ctattcgggt cagtgctaca tttctcatgc gcaaacaggg
     2461 cgtagcgcca accgtggcga ttgctcgcag gcgtgccgtt tgccatacac attgaaagac
     2521 gatcaggggc gggtggtttc ctatgaaaaa catctgctgt cgatgaaaga taacgatcag
     2581 actgccaacc tcggcgcgct gattgatgct ggtgtacgct ccttcaagat tgaagggcgt
     2641 tacaaagata tgaactacgt gaagaatatc accgcccatt atcgccagat gcttgatgcc
     2701 attattgaag aacgtggcga tctggcgcgc gcttcatcag gtcgtactga acatttcttt
     2761 gttccatcga cggaaaagac tttccaccgt ggtagcacag attattttgt gaatgcccgt
     2821 aaaggcgata ttggcgcgtt cgattcgccg aaatttatcg gcctgccggt gggtgaagta
     2881 ttgaaagtgg cgaaagatca tctcgatgtt gccgtcaccg agccactggc aaatggtgat
     2941 ggcctgaacg tgatgattaa acgtgaagtc atcggttttc gtgccaatac ggtcgagaaa
     3001 accggagaaa atcagtaccg cgtctggccc aatgaaatgc cagcagattt gcacaaaatt
     3061 cgtccacatc acccactaaa ccgtaatctt gatcataact ggcagcaggc actgacaaaa
     3121 acctccagcg aacgtcgggt ggcggtagac attgaactgg gcggctggca ggaacaactg
     3181 attctgaccc tcaccagtga agagggtgtc agcatcacgc atacgctgga cgggcagttc
     3241 gacgaagcca ataacgccga aaaagcaatg aacaatctga aggatggtct ggcaaaactg
     3301 gggcaaaccc tctattacgc ccgcgatgtg caaattaatt tgccgggggc gctgtttgta
     3361 ccaaacagtc tgttaaacca gttccgccgt gaagctgctg acatgctgga tgctgcgcgt
     3421 cttgccagtt accagcgcgg cagccgtaaa ccggttgctg agcctgcgcc ggtttatccg
     3481 caaacgcatc tgagtttcct cgcgaacgta tacaaccaga aagcgcgtga attttatcat
     3541 cgctatggtg tgcagctgat tgacgcggcg tatgaggcac atgaagagaa gggcgaagtc
     3601 ccggtgatga tcaccaagca ttgtctgcgc tttgccttta atctgtgccc gaaacaggcg
     3661 aaaggcaata tcaaaagctg gaaggcgacg ccaatgcaac tggttaacgg cgatgaagta
     3721 ttaacgctaa agtttgattg ccgcccatgc gagatgcacg tcattggcaa aatcaaaaat
     3781 cacatactga aaatgccgtt accgggaagc gtagtggcat ccgtaagtcc ggatgagctg
     3841 ctgaaaacat tgcctaagcg aaaagggtaa aacgccagtt ttctggttac tcacaactca
     3901 ttgaatctgc atgatattgc ctgccgggta aggcgtcacg ccgcatccgg catcaaacga
     3961 ctcaacgttg tcccgcttct ggtttgcgcg atttttgcca gtaaaaatgc tcgcgcaaac
     4021 cttccgccga ctcttccgcc acagcacgca attcatcgct gtccgcttca tgacgcagct
     4081 gatgatccac attctttacc cacacaaatt catgtccttt gtgccctgcc atgagttgtc
     4141 ctgagaacag cgcacacgtt aataagacaa ccgataacgc cttcgtaaac atcctgccac
     4201 ctttttctta ccttttgccg ctatgatgcc gatcgtttct tgaggttatt attcagtttt
     4261 gcaaattagc gcaaagaaat tctggaatct tccttcctga ttttgcattg cattctgtcg
     4321 ttgcggcgat ttagtgctat tttcgagcaa attacacaca gaggtaaaca ctaatgttta
     4381 ctttgttg
//
LOCUS       DAJTSL010000232         4375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTSL010000232 DAJTSL010000000
VERSION     DAJTSL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.235"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            128..457
                     /locus_tag="OO433_003903"
     CDS             128..457
                     /locus_tag="OO433_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6052202.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            705..950
                     /gene="nrdH"
                     /locus_tag="OO433_003904"
     CDS             705..950
                     /gene="nrdH"
                     /locus_tag="OO433_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6052203.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            947..1357
                     /gene="nrdI"
                     /locus_tag="OO433_003905"
     CDS             947..1357
                     /gene="nrdI"
                     /locus_tag="OO433_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6052204.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1330..3474
                     /gene="nrdE"
                     /locus_tag="OO433_003906"
     CDS             1330..3474
                     /gene="nrdE"
                     /locus_tag="OO433_003906"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6052205.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAYAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENVQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3484..>4375
                     /gene="nrdF"
                     /locus_tag="OO433_003907"
     CDS             3484..>4375
                     /gene="nrdF"
                     /locus_tag="OO433_003907"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6052206.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPYALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYSDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFF"
BASE COUNT         1077 a         1116 c         1121 g         1061 t
ORIGIN      
        1 gcgggcctac cttacctgat tgcgcattca caacgctttc agaaaagtcc ataaagccgc
       61 gatgaacctc agttaagaaa attatggtct acactgaaaa ttacatcgaa ttctaatggg
      121 ggatcatatg tttaaccgac cgaaccgcaa cgacgttgat gatggcgtgc aggatatcca
      181 gaatgatgtc aatcaattag ctgacagcct cgaatctgta ttgaagtcct ggggcagcga
      241 cgccaaaggg gaagctgaag ccgcacgcag taaagctcag gcgttgctga aagaaaccag
      301 ggcgcgaatg catggtcgta ctcgcgtcca gcaagccgcg cgcgatgccg ttggctgcgc
      361 ggattctttt gttcgtgaaa gaccctggtg tagcgtgggt acagcagctg cggtaggtat
      421 ttttatcggc gcactgttaa gcatgcgcaa atcgtagtgc aaaaatgata ataaatacgc
      481 gtctttgacc ccgaagcctg tcttcggggt ttctttttgc ctggtgaatc acaaaaatcc
      541 ccctcccccg tcacgctcat atccagggta atttcgatca ctatttgcta tatattgtgt
      601 agttgaatct tttttcaact acatctagta tctctgtatc aacagagaga caacccgacg
      661 cgtatcatcg cgccgtatct tcattttaaa cggaaatacg aatcatgcgc ataactattt
      721 acactcgtaa cgattgcgtt cagtgccacg ccaccaaacg ggcgatggaa aaccgggggt
      781 ttgattttga aatgattaat gtcgatcgcg ttcctgaagc agcggaagcg ttgcgtgctc
      841 agggctttcg tcagttgccg gtagtgattg ctggcgatct tagctggtct ggtttccgtc
      901 cggacatgat taaccgtctg catccagcgc cacacgcggc cagtgcatga gccagctcgt
      961 ctacttctcc agcagctccg aaaacacgca gcgttttatc gaacgtttag gtctgcccgc
     1021 ggtgcgcatc ccgctcaatg agcgggaacg gattcaggta gacgagcctt acatcctgat
     1081 cgtgccctct tacggcggcg gcggtacggc tggcgcggtg ccacgacagg taattcgctt
     1141 tttaaacgac gagcacaacc gggcgttgct tcgcggcgtt attgcttctg gtaatcgcaa
     1201 ctttggtgag gcgtatggcc gcgccggaga tgtgattgcc cggaaatgcg gcgtgccgtg
     1261 gctgtaccgt tttgaactca tgggtacgca aagcgatatc gaaaacgttc gtaaaggagt
     1321 aaccgaattt tggcaacgac aaccgcagaa tgcctgacgc aggaaacgat ggattaccac
     1381 gcgctgaatg cgatgcttaa cctctacgat agcgcaggtc gcattcagtt cgataaagac
     1441 cgccaggccg ttgacgcctt tatggcgacg catgtgcgtc cgaacagtgt gaccttcagt
     1501 agccagcagc agcgcctgaa ctggctggtc aacgaaggtt actatgatga aagcgttctt
     1561 aatcgctact ctcgcgactt tgtcattacg ctgtttgcct acgcacacac cagcggtttt
     1621 cgtttccaga cattcctcgg ggcatggaag ttttacacca gctatacgtt gaagacattc
     1681 gacggtaaac gttatctgga agattttgcc gatcgagtca cgatggtggc gctgacgctg
     1741 gcacaaggcg atgcgacgct ggcgttgcaa ctgaccgatg aaatgctgtc aggacgcttt
     1801 cagccagcca cgccgacatt cctcaactgc ggtaagcagc agcgcggcga actggtttcc
     1861 tgttttctgc tgcgtattga agacaatatg gagtcgattg gccgggcggt aaattccgca
     1921 ctgcaactgt cgaaacgcgg cggcggcgtg gcatttttgc tgtcgaatct gcgagaagcg
     1981 ggcgcgccaa ttaaacgtat tgaaaatcaa tcttctggcg taattccggt gatgaaaatg
     2041 ctggaagacg cattttccta tgccaaccaa ctcggcgcgc gtcagggggc aggtgcagtc
     2101 tatttacatg ctcatcatcc cgatattctg cgttttctcg atacaaaacg ggaaaatgcc
     2161 gacgaaaaaa tccgcattaa aacactgtcg ctgggcgtgg tgatcccgga tatcactttc
     2221 catctggcaa aagagaatgt gcagatggcg ctgttttcgc cctatgacgt tgagcgagtt
     2281 tatggcaagc cgtttgccga tatcgccatc agcgaacact atgacgaact ggttgccgat
     2341 gaacgcatcc gcaaaaaata cctcaacgcc cgtgatttct tccagcggct ggcagaaatc
     2401 cagtttgagt ccggctatcc ctacatcatg tatgaagaca cggtaaaccg tgctaaccct
     2461 gtcgccgggc gcataaatat gagtaatctc tgctcagaga ttttgcaggt caactgtgcc
     2521 tcagagtatg acgagaatct cgactatgcc cgcacaggcc atgatatctc ctgcaattta
     2581 ggttcgttga atattgcgca caccatggat tcccccgatt ttgcccgcac ggtagagact
     2641 gccgtgcgcg gtttaacggc ggtgtcagat atgagtcata tccgcagcgt gccgtccatc
     2701 gaagccggaa atgccgcctc acacgccatc ggactggggc agatgaattt acacggctat
     2761 ctggcgcgag aaggcatcgc ttatggttcg ccggaagcac tggatttcac caatctctat
     2821 ttctatgcca tcacctggca cgcactgcgt acctcgatgt tgctggcgcg cgaacgcggt
     2881 gaaaccttcg ccgggttcaa acagtcacgt tatgccagtg gtgaatattt tagccaatat
     2941 ctgcaaggta actggcagcc gaaaacggcg aaagttggcg aactgtttgc ccgcagcggt
     3001 attacgttac ctacccgtga gatgtgggcg caactgcgcg acgacgtgat gcgctacggc
     3061 atttataacc agaatcttca ggcggtgccg ccaaccggtt ctatctctta tatcaaccat
     3121 gctacgtcga gtattcatcc gattgtggcg aaagtagaga tacgcaaaga gggcaaaaca
     3181 ggacgcgttt actaccctgc cccgtttatg actaacgaga atctggcgct gtatcaggat
     3241 gcttacgaaa tcggcgcaga aaagatcatc gacacctacg ccgaagcgac tcgccatgtc
     3301 gatcaggggc tgtcgctgac gctgtttttc cccgataccg ccaccactcg cgatatcaac
     3361 aaagcgcaga tttacgcctg gcgcaagggt atcaaaacgc tctattacat tcgcctgcgt
     3421 cagatggcgc tggaaggcac tgaaattgaa ggctgcgtct cctgtgcact ttaaggaata
     3481 tctatgaaac tctcacgtat cagcgccatc aactggaaca agatatctga cgataaagat
     3541 ctggaggtgt ggaatcgcct gaccagcaat ttctggctac cagaaaaggt gccgctgtcg
     3601 aacgatattc ctgcctggca gacattaact gtcgtagaac aacaactgac gatgcgcgtt
     3661 tttactggcc tgacgctgct cgacacgctg caaaatgtta tcggcgcgcc ttcgctgatg
     3721 ccctatgcac tcacgcctca tgaagaagcg gtattatcga atatcagctt tatggaagcg
     3781 gttcatgccc gctcttacag ttcgattttc tcgacgctgt gtcagaccaa agatgtcgat
     3841 gccgcctacg cctggagtga agaaaacgca ccgttgcagc gaaaagctca gattattcag
     3901 caacattatc gcggtgatga tccgctgaaa aagaaaatcg ccagtgtgtt tcttgaatct
     3961 tttttgttct attccggttt ctggctgccg atgtattttt ccagccgcgg aaagctgacc
     4021 aataccgcag atctgatccg tctgattatc cgcgatgaag cagtccacgg ttactacata
     4081 ggctataaat atcagaaaaa catggaaaag atatctctgg gacaacgtga agagttgaag
     4141 agtttcgcct tcgatttgtt gctggaactc tacgacaacg agttgcaata cagcgatgag
     4201 ctgtacgccg aaaccccgtg ggctgacgat gtgaaagcgt ttctctgtta caacgccaat
     4261 aaggctttga tgaatctggg ctacgaaccg ttatttcccg cagaaatggc ggaagtgaat
     4321 ccggcaatcc tcgccgcgct ttcgccgaat gccgatgaaa atcacgattt ctttt
//
LOCUS       DAJTSL010000233         4337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTSL010000233 DAJTSL010000000
VERSION     DAJTSL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.236"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            4..546
                     /locus_tag="OO433_003908"
     CDS             4..546
                     /locus_tag="OO433_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6052207.1"
                     /translation="MATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQLHETLPDGAV
                     IHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMTGILFSLGAS
                     MVLGGVAQMLAPKDRTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVLYGEMRVGSRVV
                     SQEISTADEGDGGQVVVIGR"
     gene            607..4005
                     /locus_tag="OO433_003909"
     CDS             607..4005
                     /locus_tag="OO433_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6052208.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRVLQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTAETTYRFTQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPVAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGAGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGNVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            4072..>4337
                     /locus_tag="OO433_003910"
     CDS             4072..>4337
                     /locus_tag="OO433_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6052209.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQITRYSDTRWH"
BASE COUNT         1019 a         1089 c         1339 g          890 t
ORIGIN      
        1 gcactggcca cacagctccc ggcgtttcgt cagaaactga gcgacggctg gtatcaggta
       61 cggattgccg ggcgggacgt cagcacgtcc gggttaacgg cgcagttaca tgagactctg
      121 cctgatggcg ctgtgattca tattgttccc agagtcgccg gggccaagtc aggtggcgta
      181 ttccagattg tcctgggggc tgccgccatt gccggatcat tctttaccgc cggagccacc
      241 cttgcagcat ggggggcagc cattggggcc ggtggtatga ccggcatcct gttttctctc
      301 ggtgccagta tggtgctcgg tggtgtggcg cagatgctgg caccgaaaga cagaactccc
      361 cgtacacaga caacggataa cggcaaacag aacacctgtt tctcctcact ggataacatg
      421 gttgcccagg gcaatgttct gccggttctg tacggtgaaa tgcgcgtggg gtcacgcgtg
      481 gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt
      541 cgctgatgca aaatgtttta tgtgaaaccg cctccgggcg gttttatcgt ttatggagca
      601 tgacgaatgg gtaaaggcag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      661 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aagggccggt tgaaggtccg
      721 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaggggaat
      781 accaatatat ccggcgtcac ggtggtgttc cgggccggtg agcaggagca gacaccgccg
      841 gagggatttg aatcctccgg ttccgagacg gtgctcggta cagaagtgaa atatgacacg
      901 ccgatcaccc gcaccattac gtctgcaaac atcgaccgtc tgcgctttac cttcggtgta
      961 caggcactgg tggaaaccac ctcaaagggg gacaggaatc catcggaagt ccgcctgctg
     1021 gttcagatac agcgtaacgg tggctgggtg acggaaaaag acatcaccat taagggtaaa
     1081 accacttcac agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgttcagt
     1141 atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa caaaacgctc
     1201 tggtcgtcat acaccgaaat tatcgatgtg aaacagtgct acccgaacac ggcactggtc
     1261 ggcgtgcagg tggactcgga gcagttcggc agccagaagg tgagccgtaa ttatcatctg
     1321 cgcgggcgtg ttctgcaggt gccgtcgaat tataacccgc agacgcggca atacagcggt
     1381 atctgggacg gaacgtttaa accggcatac agcaacaaca tggcctggtg tctgtgggat
     1441 atgctgaccc atccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa
     1501 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag tgccggacgg ctttggcggc
     1561 acagagccgc gcatcacctg taatgcctgg ctgaccacgc agcgtaaggc gtgggatgtg
     1621 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     1681 ttcgtgcagg accgaccatc agataaggtg tggacctata accgcagtaa tgtggtgatg
     1741 ccggatgatg gcgcgccgtt ccgctacagc ttcagcgccc tgaaggaccg ccataatgcc
     1801 gttgaggtga actggattga cccggataac ggctgggaga cggcgacaga gcttgtggag
     1861 gacacgcagg ccattctccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     1921 accagccggg ggcaggcaca ccgcgccggg ctgtggctga ttaaaacgga gctgctggag
     1981 acgcagaccg tggatttcag cgtcggcgca gaagggcttc gccatgtgcc gggtgatgtt
     2041 attgaaatct gcgatgatga ctatgcgggg atcagcatcg gcgggcgcgt gctggcggtg
     2101 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccatc ctccggtacc
     2161 acgctgataa gcctggttga cggaagtggc aatccggtca gcgtggaggt ccagtccgtc
     2221 accgacggcg tgaaggtaaa agtgagccgt gttcctgacg gcgttgccgg atacagcgta
     2281 tgggggctga agttgccgac gttgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     2341 aacgatgacg gcacgtatgc catcaccgcc gtgcagcatg taccggaaaa agaggccatc
     2401 gtggataacg gggcgcactt tgacggcgac cagagcggca cggtgaatgg tgtcacgccg
     2461 ccagcagtgc agcatctgac cgccgaagtc actgcagaca gcggggaata tcaggtgctg
     2521 gcgcgatggg acacgccgaa ggtggtgaag ggcgtgagct tcctgcttcg cctgaccgtg
     2581 gcagcggatg acggcagtga gcggctggtc agcacggccc ggacggcgga aaccacatac
     2641 cgcttcacgc agctggcgct gggacggtac acgctgacag tccgggcggt aaatgcgtgg
     2701 ggacagcagg gcgatccggc gtcggtatcg ttcaggattg ccgcaccggt agcaccgtcg
     2761 cggattgagc tgacgccggg ctattttcag ataactgcca cgccgcatct tgcggtttat
     2821 gatccgacgg tacagtttga gttctggttc tcggaaacgc ggattaccga tatcaggcag
     2881 gttgaaacca cagcccgcta ccttggcgcg gggctgtact ggatagccgc cagtatcaat
     2941 atcaaaccgg gccatgatta ttacttttat atccgcagtg tgaacaccgt tggcaaatcg
     3001 gcattcgtgg aggccgtcgg tcgggcgagc gatgatgcgg aaggttacct ggattttttc
     3061 aaaggcaaga taaccgaatc ccatctcggt aaagagctgc tggaaaaagt tgatctgacg
     3121 gaggataacg ccagcagact ggatgagttt tcgaaagagt ggaaggacgc taacgataaa
     3181 tggaatgcga tgtggggcgt caaaattgag cagaccaaag acggcaaaca ttatgtcgcg
     3241 ggtattggcc tcagcatgga ggacacggag gaaggcaagc tgagccagtt tctggttgcc
     3301 gctaaccgta tcgcgtttat tgacccggca aacgggaatg aaacgccgat gtttgtggcg
     3361 cagggcaacc agatattcat gaatgacgtg ttcctgaaac gcctgacggc tcccaccatt
     3421 accagcggtg gaaatccgcc ggcattttcc ctgacaccag acggaaagct gaccgctaaa
     3481 aatgcggata tcagcggtaa tgtgaatgca aattcaggga cgctcaacaa tgtcacgatt
     3541 aatgaaaact gtcagattaa ggggaaactg tcagccaatc agattgaagg cgatattgtc
     3601 aaaacggtca gcaagtcttt cccccgcacg aacagttatg ccagtggcac catcacggta
     3661 agaatcagtg atgatcagaa atttgaccgg caggtcatga taccgccagt gttattccgc
     3721 ggtggtaagc atgagaattt caacagtaat aaccaacagt catactggta ttcaacctgc
     3781 cggttaagag tgacccgcaa tggtcaggag atttttaatc agtccacgac ggatgctcag
     3841 ggcgtatttt cctcagttat agatatgcct gccggacagg ggacgctgac actgacattc
     3901 accgtatctt catcaggagc gaataactgg acaccaacaa ccagtatcag cgatctgctg
     3961 gttgtggtga tgaaaaaatc cacagcaggt atcagtatca gctgaatttt ataacccaga
     4021 acgggcgtca gaaatgacgc cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa
     4081 ctttatgccg ccattttgtc cgcagccatt tgtctggccg tatccggtgc gcctgcatgg
     4141 gcgtctgaac atcagtccac gctgagcgcg gggtatcttc atgcccggac gaacgttccc
     4201 ggcagtgatg atctgaacgg gattaacgtg aaataccgtt atgagtttac ggacacactg
     4261 gggatggtga cgtcgttcag ctatgcagga gacaagaatc gccagattac ccgttacagc
     4321 gatacccgct ggcatga
//
LOCUS       DAJTSL010000234         4320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTSL010000234 DAJTSL010000000
VERSION     DAJTSL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.237"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..976)
                     /locus_tag="OO433_003911"
     CDS             complement(<1..976)
                     /locus_tag="OO433_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR6052210.1"
                     /translation="MIRKSATGVIVALAVIWGGGIWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEV"
     gene            complement(1131..1757)
                     /gene="dsbA"
                     /locus_tag="OO433_003912"
     CDS             complement(1131..1757)
                     /gene="dsbA"
                     /locus_tag="OO433_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6052211.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(1774..2760)
                     /gene="srkA"
                     /locus_tag="OO433_003913"
     CDS             complement(1774..2760)
                     /gene="srkA"
                     /locus_tag="OO433_003913"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6052212.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRIVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDSTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(2837..3106)
                     /locus_tag="OO433_003914"
     CDS             complement(2837..3106)
                     /locus_tag="OO433_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6052213.1"
                     /translation="MKCKRLNEVIEFLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            3176..3760
                     /gene="mobA"
                     /locus_tag="OO433_003915"
     CDS             3176..3760
                     /gene="mobA"
                     /locus_tag="OO433_003915"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6052214.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            3742..4269
                     /gene="mobB"
                     /locus_tag="OO433_003916"
     CDS             3742..4269
                     /gene="mobB"
                     /locus_tag="OO433_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6052215.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGARHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         1169 a         1021 c          966 g         1164 t
ORIGIN      
        1 caacttcatc gtttgctaac tcagggtgtg cagcaagctg cttctgcatc gcaatgttat
       61 actggatgat aaattggcgc actgctgagg gatcaataga ttcagcaatc aatgaccact
      121 caccactacc gagttgctga ttcccttttg ttaacttatc aagttcgtag gtcagattga
      181 cattgatata atctttgtct ttaacgcgat ccagcctggt ttttgcaacg aaagcatcga
      241 tttgggcaac gtcttccccc tgattgataa tattaacctg attaaatttc gactcgcttt
      301 caccaaccgg aaatttcttt tcttctaatc gtgtcagaga atcaatggta aaactttttg
      361 ccgttacttt gttcattggt gataattgca gaacaatatc ttcgactttg ccttcaatat
      421 ccaggttaga caatgaacta ccatcaccat taaaagtaaa ctggccctca ccaaaagaaa
      481 actttccata ctctgcaggg gcaacatcta atgtcgtaga gaatgagcca ccaaacgcga
      541 atcgcaatgt cgcttccacg ggcgatttat ttttcgcggc gataaatagc ggttgtgtca
      601 gttcattatt cactaagttc acttttgccg ctgccagtgc tgggatgaca ttaccatgca
      661 ttaacattgt gatgggcaac ggaccgtgct caacatccac atcaaatgca actttctggc
      721 ctggcttgat attgagatcg ggtgcaccgt tatcaaatga aatttgcatt tgaaaatggg
      781 aattaaaaaa gcctttgtca aagtttttat aacttaacgt catatcgtag gcatgttcac
      841 ctttcttttt agcatcgtta aaatctttaa taaatttttc gacaccaggc tgaatttgcg
      901 tacctgtgta ccatatgcca ccaccccaga ttacggctaa cgcaacaata acacctgtag
      961 ctgacttacg tatcatataa atattccctg ataaaacagc aaaactttga atatccactt
     1021 atgctgaaat aatgtccagc ggcaggatgt attatcagaa tgtatttata tttattaaat
     1081 aataaaaaaa gcccgtgaat attcacgggc tttatgtaat ttacattgaa ttattttttc
     1141 tcggacagat atttcactgt atcagcatac tgctgaacaa aaacatccat attgctggta
     1201 tccataccct gcggattcag ctgatattta ccgttaacaa acatcgccgg aacaccgcgc
     1261 aattgcacgt cagctgcagc tttttcctgc tgagctacca gagatttcac cacgaagctg
     1321 ttccacgccg cgtcgtactc ttcaccttta atacctgcgt tgataaatac atcgcggata
     1381 tcagaagcag aacgaatggt ctgggttttc tgtacgcctt caaacagcgg aacagtcact
     1441 ttgtcttcca cgcccagcgc catcgccaca gcccatgcct gagtcagatc tttgcccagg
     1501 tcaccaccca tgaagttgac gtggtattta gtcatcttca cgccttccgg cagttttttc
     1561 ttcacattat cagaaatatg cagaacttct tcaaactgat agcagtgcgg acagaagaaa
     1621 gagaaaaact ccagcacttg cggcgcgcca gctaccggtt tttccagggt agtgtactgt
     1681 ttaccatctt catactgcgc cgccgatgcg ctaaacgcta aaactaaacc agccagcgcc
     1741 agccaaatct ttttcatgat ctactctctc cgattaatac ataggtgtta attgtaaagg
     1801 gggttcttgt agaacttttg cctgttctat aaaagtcgcc gtttgtcgca gccagtaatc
     1861 ttccccggtt aaccacggga aatttttcgg gaacgcggga tcagcccaac gccgcattag
     1921 ccaggcaaga taataaacca aacgcatggc gcgtaaaggt tcaatcagtc cgatttcagc
     1981 ggtgtcgaac tcgctaaatt cttcataagc ttcaataata gtttccagtt gcatccgctg
     2041 ctcggcttta tcgccattga gcaacatcca caaatcctga atagctggac cattacgtgc
     2101 atcatccaga tcaacaaaca ttggaccatc gcgccagaga atattcccgg cgtggcagtc
     2161 tccatgtaag cgcagaacgg tggaatcttc ccgccagtgc gctgtaacgg cggcgatcag
     2221 ctcatctgtc gctttcagga atgccgcttt caacccggaa ggtatcagtg tagcgtcctc
     2281 aaacagcttg cgtggctcaa tgagatattc attcagaccg atggtcgggc gatggataaa
     2341 aagctgtttg cgccccgtct ggtgcatacg ccctaaataa cgcccaaccg cttccatctg
     2401 atcgatatta tcagcttcga actggcgacc accgacgctt ggaaaaacag cgaaataaaa
     2461 tccctgatga ttcaataaag tctgaccgtt aaaggccaca ggtgctgcga ccggaacttc
     2521 atcatttacc aactgcaacg caaattgatg ttcttcgagg atttgatcgg ctgtccaacg
     2581 ttcagggcga taaaatttga cgacaatacg tcgacgatct tcgtcctgaa attgatagac
     2641 acggttttca tagctgttaa gcggggtaag accggaatcc acccggatcc cttgctcaaa
     2701 tagagcatcc atgatggtat ccgggtgtag tgtctggaaa gtaaaagcgc tgttgttcat
     2761 cccatcatcc ggaaaacatt acgaatgatg aaggatatca tttcacgatg attttggtgg
     2821 cgccgcttac aagcttttac tctttaatta cgccgcgagc gcgtaacagc gccgttttga
     2881 agtcttcttc ataatctttc tgcaaacccg ggatcaccgc atctttcgcg gaatcacgca
     2941 ttttcaggtg atagatcaga atgtcatccg tcaaatccgc cagttcgccg tcaaaacctg
     3001 actctttcgc cagtttctgc aaaaattgca acaggttaag gtctggctct ttttgccagg
     3061 ctggctggag gaattcaata acttcattca gacgtttaca tttcatagtg atgctcctta
     3121 ctcttgagac agacacgtta gcagggtcaa tcccacaata aaagaggcga tatcggtgaa
     3181 tctgatgacg acgataacag gcgttgtgct ggcaggcggt aaagccagac gaatgggtgg
     3241 cgtagataaa ggattgcttg aattaaacgg caaaccatta tggcaacatg tcgctgacgc
     3301 gcttatgacg cagctctctc acgtcgtggt taatgccaat cgtcatcagg aaatctatca
     3361 ggcaagcggt ctgaaagtga ttgaagattc actggcggat tacccaggcc ctctggcagg
     3421 aatgctttca gtaatgcagc aggaagctgg tgagtggttt ttgttttgcc cgtgcgatac
     3481 gccttacatt ccccctgatt tagcagcccg gcttaatcat cagcgcaaag atgcgcctgt
     3541 cgtgtgggtc catgacggtg aacgcgatca cccgactatt gctctggtaa accgcgctat
     3601 tgagccttta ttactggaat atctgcaagc aggagaacgc cgggtaatgg catttatgcg
     3661 tctggctggc ggtcatgcgg ttgatttcag cgatcataaa gatgcatttg ttaacgtgaa
     3721 tacgccagag gagcttgccc gatggcagga aaaacgatga taccgttact cgcctttgcc
     3781 gcgtggagtg gtaccggaaa aactacgctt ttgaaaaaac tgatcccggc attatgcgcc
     3841 agagggatcc gtccagggct gattaagcat acgcaccatg atatggatgt tgataagcca
     3901 ggcaaagata gctatgagct gcgcaaggct ggcgcggcac aaaccatcgt tgccagccag
     3961 caacgatggg ccttgatgac ggaaacacca gacgaagaag agctggatct acagtttctc
     4021 gcaagtcgaa tggatacctc aaagctggat ttgattctgg tcgaagggtt taagcatgaa
     4081 gagatcgcaa agattgtgct gtttcgcgat ggagccagac atcgacctga agaattagtg
     4141 atagacaggc atgttattgc tgtagccagt gatgtgccgc ttaatcttga tgtcgcatta
     4201 ctggatatta atgatgttga ggggctggct gattttgtgg tggagtggat gcaaaagcag
     4261 aacggataag caagagtagg ctggatcaga cgcagccagt gtcgcatccg acctttttct
//
LOCUS       DAJTSL010000235         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTSL010000235 DAJTSL010000000
VERSION     DAJTSL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.238"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO433_003917"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO433_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6052216.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO433_003918"
     tRNA            complement(324..399)
                     /locus_tag="OO433_003918"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO433_003919"
     tRNA            complement(406..480)
                     /locus_tag="OO433_003919"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO433_003920"
     tRNA            complement(597..681)
                     /locus_tag="OO433_003920"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO433_003921"
     tRNA            complement(690..765)
                     /locus_tag="OO433_003921"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO433_003922"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO433_003922"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6052217.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAAQRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO433_003923"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO433_003923"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6052218.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO433_003924"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO433_003924"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6052219.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLLKEWQPVLTYGDLTRLDPTTVTPQQVFNVVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1079 a         1040 c          932 g         1132 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttcttcttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctaggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctcagcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gtaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc acattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttagcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tag
//
LOCUS       DAJTSL010000236         4118 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTSL010000236 DAJTSL010000000
VERSION     DAJTSL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.239"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(26..583)
                     /gene="ymfL"
                     /locus_tag="OO433_003925"
     CDS             complement(26..583)
                     /gene="ymfL"
                     /locus_tag="OO433_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HCR6052220.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAKLPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDIEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(627..827)
                     /locus_tag="OO433_003926"
     CDS             complement(627..827)
                     /locus_tag="OO433_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6052221.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTQGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            918..1592
                     /locus_tag="OO433_003927"
     CDS             918..1592
                     /locus_tag="OO433_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR6052222.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPIGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALHPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            3122..3658
                     /locus_tag="OO433_003928"
     CDS             3122..3658
                     /locus_tag="OO433_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HCR6052223.1"
                     /translation="MSFIQTLTGKKFNYINSTIDDIYIEDIAGALSNICRFAGHLPDF
                     YSVAQHSVLCSQLVAPEFAFEALMHDATEAYCQDIPAQLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPLLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            3649..4011
                     /locus_tag="OO433_003929"
     CDS             3649..4011
                     /locus_tag="OO433_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052224.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     LENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGDWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
BASE COUNT         1254 a          897 c          873 g         1094 t
ORIGIN      
        1 cgacggtagt gtgttgttaa actgttcatg cgttagtttc tccacaacca gaagcaatcg
       61 acgccacgac gcccggagct gcacactcgc gggcgtcatt actttctgaa atgcaaaaaa
      121 ttttgtagac aagtgctgca tgctcctgca gcttcgaaat tgagagatac agctcgtcgt
      181 taattgctgt cttctcatgc ggttccacta caccgtcttc gattgctgaa cgaatctgtt
      241 ttgaataact gccgatctgt tcaatgactt ccagcagacg ctggttaata tcggcgttgt
      301 ccacatcctc gatgtcagga agagacacaa agacgccatt tgcagactgc gccacagcat
      361 cagcaatgaa gtgagtgcca ccagcacgct gtaaaaccat tgcccatccc agcgggaaaa
      421 tctgatcgcc atctgcacga aggcggttga ataaagcgtt ttctgttaca tcgagccagt
      481 cagccgcttc agcgtaacca cccggcaact ttgcgatagt ttttctgaca gctttcacgt
      541 accactcagg ctgtttttct attttccagt gatgcttacc cacgattagc ctcatcgttc
      601 tgtggttaaa aattgaaagt gttctgctaa tctttcggat agatatccgg tcttaagtca
      661 gatttcgtaa ttgcacctga cgtgcattgc tcaagttttt tagccagcac aaaactggct
      721 tttttatagc cattgaaaac cagccgtaag tagccttgtg ttgagccaac ttttccggcc
      781 aactcaccct gctgttcttt ggttaaagag tcccaatacg ctttcataca atatgtacct
      841 ccagtgtaca catcacatga ttgaaatgaa ccttcaagat acttgtacct tagcggtaca
      901 agggttttaa tttcgttatg aaaacaatcc atgacatccg gcggtctaac gccagaaaac
      961 tgagagatgg tgttggcggg aattcttcct ttgccactat gattgatcgc gagccaaccc
     1021 agaccagcag gtttatggga gatggtgcta ctaaaaatat cggtgacagc atggcacgac
     1081 acattgaaaa atgtttcgac ctgcctatcg gatggctcga tcaagaacac cagacaacga
     1141 acatcacaaa aaaacctgat gtttcaatca ctaacaaaca aataacgtta gtccctgtca
     1201 tatcatgggt acaggccgga gcatggaaag aagttggcta ttctgaggtt gatttgagca
     1261 cagcagaaac ttatccctgc cctgtaccct gtggcgaaat gacttatatc ttgcgggtga
     1321 ttggtgattc aatgattgat gagtaccgtc cgggagacat gatttttgtt gatcctgaag
     1381 tccctgcttg ccacggtgac gacgttattg cgttgatgca cgatacaggc gaaaccacct
     1441 tcaagcgatt gatagaagat ggaacacagc gttatctcaa agcattacac ccaaactggc
     1501 ctgagcccta cattaagatt aacggtaatt gctctataat tggcactgtg attttctcgg
     1561 gaaaaccaag aagatacaaa ataaaggcct aatcaatatt tatgaacctg cttcggcagg
     1621 tttttttata cttgacaatg taccccatgg acacacaatg tacccccatg gacacacaat
     1681 gtaccaaaac gaaacaacaa acaggcagga tgcccacgaa gtagccgcct ggggcatatg
     1741 aagtccaaga tgattcgtta gcaacaaaac gcccatagga cgcttcgctc tttaacaatt
     1801 tgaatatttt cagcgtcaat gatctgttta gctccctaca agtaaatgta gcgtagcacc
     1861 atgataaatc ctgctagtgt ttagttaaaa atcgcgcgag agcaagaacg acgtgagggc
     1921 aggcaatact gatagtgtta ttgctattag tttatcaaaa atatttagac gacccagatg
     1981 aatattttta atacatactc cccctaattt aacaggagta aatgttgatg aataacgaga
     2041 catctatact caacaattgt atgatatgaa caattatata acataatgta ggaaaaataa
     2101 aaatggattg gcatgacctt atatcaaagg taattatcgg acttgttaca ggctcaatcg
     2161 cagcttactt aactgcgcga tatgcactcg gccgttatta taaagaaaaa tggtgggaca
     2221 aaagaataga tttgtatacg taattagttg actgtgttta tcttctaaaa aacacagctg
     2281 actattggta taaacatgag ttacatcacg cttcacaatt cccaacctac tttagttaca
     2341 aatcaaaaga agaaaatgaa atccttagca aatctttctc taatgagctt gaaaaactca
     2401 gtagaatagg tgagctatct tcattattct taacagaaga gtgtggggat aaaatccgtt
     2461 cattcattga aaatgaagaa aatctattta tacaatgggt gtttgatagt atagagttga
     2521 tagatgccca caagtcaaga cttgataaat taaaagtatt actagacgat attatctctg
     2581 aagcaaagag agagcttaag gtcaataaaa aacataacag cctaaagaat ataaagtggt
     2641 ccataactat gccagaccaa aaaaatgatg agccaattaa ttatcacgac aatcattaaa
     2701 acactttaga acccgctggg cgggttctat tcaaatatac tttgcacatc acaaataaaa
     2761 ctataacgtc cttcttcttt tcctaatatc ttatatctac ttatttataa gcggcttaca
     2821 gatcttcaac agataaacct aatgaaggcg acaatgttct gatgaatagc caccttttta
     2881 ctcaactgaa agcgcgttct gtcccttgtc attaagtgcc agttcgttaa gtccaaaacc
     2941 tccggaacgc gctttcaatt gagtggggaa aataactggc gatggcagcc gcccgttgca
     3001 ctaagtgcct ctggatgggg catttactga aacgaaacca aaacttttaa tcgccttatg
     3061 gcgagggatt cgtgcaacca aaaatcgtgc ggtgcagcgc accaattaag gagaactaac
     3121 gatgagtttt atccagactc ttaccggtaa gaagttcaat tatatcaact ccaccattga
     3181 cgatatttat atcgaggata tcgccggtgc gctttcaaat atctgccgct tcgcgggaca
     3241 cttgccggat ttttacagcg tggcgcagca ttccgttctg tgcagtcaac ttgtggcgcc
     3301 agagttcgcc tttgaagcgt taatgcatga tgcaacagaa gcgtattgtc aggacatccc
     3361 cgcgcaactg aaacgccttc ttcctgacta taaacggatg gaagaaaaaa tagacgccgt
     3421 aatccgtgag aaatacgggt tacccccggt tatgagcacg cccgtgaaat atgccgatct
     3481 catcatgctg gcaaccgaac gccgcgatct tgggcttgat gatggctctt tctggcctct
     3541 actagaaggt atcccagcaa cagagatgtt caaagttatt ccactgtcgc caggccatgc
     3601 ctacgggatg tttatggaac gttttaacga attatcggag ttacgcaaat gcgcatgaat
     3661 gttttcgaaa tggaagggtt tcttcgcggg aaatgtgtac cgcgagatct gaaagtgaat
     3721 gaaacggatg ctgaatacct ggtgcgtaaa ttcgatgcgc ttgaagctaa atgtgcggca
     3781 ctggaaaaca aagtaatacc agtgtcaact gaactgccac cagcaaatga aagtgttttg
     3841 ttattcgatg ctaacggaga aggctggcta attggctggc gttctctctg gtacacctgg
     3901 ggacaaaaag aaaccggaga ctggcagtgg acatttcagg tcggggacct tgaaaacgtc
     3961 aatatcactc actgggcagt aatgccaaaa gcaccggagg ctggagcata atgaccactt
     4021 ttaccgacaa agaactgatt aaagaaatta aagagcgtat cagcagcctt gacgtgcgag
     4081 acgatattga gcgccgtgct tatgaaatcg cactccta
//
LOCUS       DAJTSL010000237         4070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTSL010000237 DAJTSL010000000
VERSION     DAJTSL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.240"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1985
                     /gene="ybiO"
                     /locus_tag="OO433_003930"
     CDS             <1..1985
                     /gene="ybiO"
                     /locus_tag="OO433_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR6052225.1"
                     /translation="SRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEVSR
                     HYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCALPL
                     YRKMGLWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRTIA
                     FQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIGYG
                     LIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLAFF
                     SLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSGMF
                     SRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFDFW
                     NWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRTLL
                     TLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGVFI
                     QFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGIGS
                     VVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTLRV
                     SFTTLPLKQWTVRFALDSQVKKHFDLVGVRAPVQTY"
     gene            complement(2045..2971)
                     /gene="rlmF"
                     /locus_tag="OO433_003931"
     CDS             complement(2045..2971)
                     /gene="rlmF"
                     /locus_tag="OO433_003931"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6052226.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3247..3506
                     /locus_tag="OO433_003932"
                     /pseudo
     CDS             3247..3506
                     /locus_tag="OO433_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            3771..>4070
                     /locus_tag="OO433_003933"
     CDS             3771..>4070
                     /locus_tag="OO433_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6052227.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT          945 a         1064 c         1022 g         1039 t
ORIGIN      
        1 cctcgcgtaa agaattgatc gaccagttgc gtactgtcgc cgcgacgccc cctgcggaac
       61 cggtcccgaa gatcgtgccg ccgacgctgg ttgaagagca aaccgtgctg caaaaggtca
      121 ccgaagtcag ccgccattat ggtgaagccc tttccgcccg cttcgggcaa ctttatcgca
      181 atattaccgg ctccccgcat aagccgttta atccacaaac attcagcaat gcgctgaccc
      241 atttttcaat gttagcggta ttagtgtttg gtttttactg gctgattcgc ctgtgcgcac
      301 tgccgctgta tcgcaaaatg ggcctgtggg cgcggcaaaa aaatcgtgag cgcagtaact
      361 ggttgcagct tccggcgatg attatcgggg cgtttattat cgacctactg ttactggcac
      421 tgacattatt tgtcggccag gtattaagcg acaacctgaa tgcaggcagt cgcaccatcg
      481 ctttccaaca aagtttgttt ctcaacgcct ttgccctgat tgaatttttc aaagctgtac
      541 tacgcctgat tttttgccca aacgtggctg aactgcgccc gttcacgatt catgacgaga
      601 ccgcccgtta ctggagtcgt cgcctgagct ggttaagcag tttgataggt tatggcctga
      661 ttgtggccgt gccgattatc tctaatcagg tgaatgtaca gataggtgcg ctggcgaacg
      721 tcatcattat gctgtgcatg accgtctggg cgttgtacct gatctttcgc aataaaaaag
      781 agattaccca acatttgctc aactttgcgg agcattcgct ggcctttttc agcctgttta
      841 tccgcgcctt tgcgctggtg tggcactggc tggcaagcgc ctattttatc gtgctgtttt
      901 tcttttcgtt gttcgatccg ggcaacagcc tgaaatttat gatgggtgca acggtgcgca
      961 gcctggcgat tattggtatc gcagcgtttg tttccggtat gttttcccgc tggctggcga
     1021 aaaccatcac cctctcgcca catactcagc gtaactatcc ggagttgcaa aaacggctga
     1081 atggctggct ttcggcggcg ctgaaaacgg cgcgtattct gaccgtctgc gtggcggtaa
     1141 tgttgctgtt gagcgcatgg ggattgttcg atttctggaa ctggctgcaa aacggcgcgg
     1201 ggcagaaaac cgtagatatc ctgatccgta tcgcacttat tctttttttc tcggcggttg
     1261 gctggacggt gctcgccagt ttgatcgaaa accggctggc ttcggatatt catggccgcc
     1321 cgctacccag cgcccgtacg cgtaccctgc tgacgctgtt tcgtaacgcg ctggcggtga
     1381 ttatcagtac catcaccatc atgattgtgt tgtcggaaat cggcgtcaat atcgcgccat
     1441 tgctggcagg tgccggggca ttaggtctgg ctatctcgtt tggttcgcaa acgctggtga
     1501 aagatatcat taccggggtg tttattcagt ttgaaaacgg catgaacact ggagatttgg
     1561 tgactatcgg gccgttgacc ggcacagtgg aacggatgtc gattcgctcc gtgggcgtgc
     1621 gccaggatac cggggcgtat cacatcattc cgtggtcttc gataaccacc tttgctaact
     1681 tcgtccgcgg tattggttcg gtagtggcaa actacgatgt tgatcgccat gaagatgctg
     1741 ataaagccaa tcaggcactg aaagatgcgg tagcggaatt gatggaaaac gaagaaattc
     1801 gcgggctgat tattggtgaa ccgaattttg ccgggattgt cggcttaagc aataccgcgt
     1861 ttacactgcg tgtttcgttc accacgctgc cactcaaaca gtggacggta cgctttgccc
     1921 tcgacagcca ggtgaaaaaa catttcgacc tggtgggcgt tcgcgcgcca gtgcagactt
     1981 attaggtgct gcctactccg ggcgcgaccc cggctgaacc gttaccgccg ggggagccaa
     2041 cgctttaacg ctggcgattg acaaaacggc ggcgctgctc gtcgttcata aaggtccagg
     2101 caataaagcg actctgcttt tgcccctggg ccatctcttt ttttaccacc ttcaccgcgc
     2161 ccacgtcggt cagagcacga tacaacggcg gtaagttttc accacgagat accagcgagg
     2221 taaaccacat cacctgcttc gcgaagcctt tgctctcttc aatcatcttt ttgataaagg
     2281 cgacttcacc accttcacac cacaactcct gttgctgacc accaaagttc agtgcatcgt
     2341 ctttgttcag ccccaggtta cggcgtttac gctcactacc tgcccgcgct gcagcggcgg
     2401 aatcgtggaa tggcgggtta cacaaggtcg cgtcgtattg ctcgttttta tggatgatgc
     2461 cgttaaaaat tgccccactc tctttttgcc gacgcagacg aatggcgcgg ttaagccccg
     2521 gattagcact gataatcgcc tgcgcactgc ttaacgcctg gctgctggtt tcactaccgg
     2581 taaaacgcca gccatattca tgtacgccaa tcagcggata aatacagttc gcaccaacgc
     2641 cgatatccag aatgctggca ttcgctggaa ttgttccgct tgcttcagcc agtaaatcgg
     2701 caaggtgatg aatataatcc gcccggccgg gtactggtgg gcagagaaaa ccgtcgggga
     2761 tatcccagtt cgctacggcg taaaaatggg ccagcaacgc cttattgagc gccttcaccg
     2821 ccagcggatt ggcaaagtct acgctttgct ccccggcggg tgtaagcgtg aggaattgcc
     2881 tgagttcagg attgacctga caaagcgtgg cgagatcgta gcggctgtga tgacggttgc
     2941 gcggatgcaa ccccggtttc tgggcggaca tagcattctc ctttaagcag cggcgtaaga
     3001 tacccgcata acgcgacagg gtaaatatct ggctgcgttt atcattcccc aaaaccttaa
     3061 catggctatg gtttatggca attcatcata aaaatcagca ttatgcatgt cattgatttg
     3121 tcttattttg taaaaatata tcgttcaaaa aataagcaca tctctgtcaa ttttttgcgt
     3181 catttgcatc gcgcaggcga cctacactta tcaggcacta cccgttgtat ttatgaggat
     3241 gtcattatga aaaagtgcct cacactactg attgccaccg tcctgagcag aatctctctc
     3301 acggcttatg ccgcgcaacc gatgagtaac ctggacagcg gtcagctacg gcccgcggta
     3361 ccgtttcggc aactggcgca tcaaacctaa gcgatctgga ggataaactg gcagaaaaag
     3421 cgcgcgaaca aggcgcgaaa ggttatgtca ttaattccgc aggcggaaat gaccagatgt
     3481 tcggtactgc aaccatctac aaataacccc accaacgtaa ctgcattcgc ctctggatgc
     3541 agttttcgcc tgttttgtaa tcaaaaaaca tcaaatatga actcaatgta aataaatgta
     3601 tttctttttc gcgcaatggg tgatagaaaa tcgcgccaag tgataatgct tatcaaaatt
     3661 attatcactt gcacgaacac tatcacggga ttaacagtgg catcgcatcc gcagagatgc
     3721 tttctcgtga cagtgaaaat ttcaacatat aagaaaaagt cacctgcaaa atggaaaaca
     3781 atcgcaattt ccctgccaga caatttcatt cgctcacgtt ctttgccggt ctttgtattg
     3841 gcatcacgcc tgtggctcag gcactcgccg ccgaagggca aactaacgcg gatgacacgc
     3901 tggttgtcga agcatcaacg ccttcgcttt atgcgccaca acaatctgcc gatccgaaat
     3961 tctcgcgtcc ggtagcggat actacccgca cgatgacggt gatttctgaa caagtgatta
     4021 aagatcaggg cgcaaccaac cttaccgacg cgctcaaaaa cgtccccggc
//
LOCUS       DAJTSL010000238         4068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTSL010000238 DAJTSL010000000
VERSION     DAJTSL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.241"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1036)
                     /locus_tag="OO433_003934"
     CDS             complement(<1..1036)
                     /locus_tag="OO433_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6052228.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEEN"
     gene            1527..1637
                     /locus_tag="OO433_003935"
     CDS             1527..1637
                     /locus_tag="OO433_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR6052229.1"
                     /translation="MLIGYVRVSTNDQNTDLRRNALNCAGYELIVERMGA"
     gene            1917..2912
                     /gene="pgl"
                     /locus_tag="OO433_003936"
     CDS             1917..2912
                     /gene="pgl"
                     /locus_tag="OO433_003936"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6052230.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTIEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHLISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2953..3905)
                     /locus_tag="OO433_003937"
                     /pseudo
     CDS             complement(2953..3905)
                     /locus_tag="OO433_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1091 a          826 c          953 g         1198 t
ORIGIN      
        1 aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga tctgcttcag
       61 gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc cccccttcag
      121 tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt aaaaacagtc
      181 tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga accgaagttt
      241 gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc tcaatagtta
      301 tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg ttttcaaaat
      361 aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt aatctattgc
      421 tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta agatggttgt
      481 gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc agttcgttgt
      541 tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat aacctattat
      601 actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct aattgatttt
      661 cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt aagaaattat
      721 ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt aatttattac
      781 agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa agcgccaagg
      841 ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt cttaccgcct
      901 cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg gacaaataat
      961 ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata tttctgagca
     1021 tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat ttaatgtatc
     1081 tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg ttatatcatg
     1141 ctttctatcc tggtaagtat atttgctttt ttcgtatatt tggttcatct ttttttgatg
     1201 ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg agtaagatac
     1261 tacaacatct ccaactattt tagccagcct gactacttga cttggtaata tttgttgcta
     1321 ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat ttagtaatga
     1381 tccaaatcat atatcaatgt actgtttttt tctttaaaaa aacagtacat tctattttat
     1441 aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac ttttgacgca
     1501 gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta tcaacaaatg
     1561 accagaacac cgatctacga cgtaatgcgt tgaactgtgc aggatatgag ctgattgttg
     1621 agcgaatggg agcttgattg gaagttgtta gggtatggtg tttacggtcg ttgatatggc
     1681 gaacaaacgc ggggagcgtc acgctcccta aatggtcgcg aaaatatctg tggcattgtc
     1741 aaatttgtga cggtgactaa tattttaatg atatgccatt cgaaatggtg tgctaacgta
     1801 ttgatttaat tgtgacgcga tgttgtattt tatcgcgttt tgttgtctgt ttttcattcg
     1861 cttgatgttg ctatttacat gaaaaataaa gaatttattg tttttgaggt gtgtttatga
     1921 agcaaacagt ttatatcgcc agccctgaga gccagcaaat tcacgtctgg aacctgaatc
     1981 atgaaggcgc actgacgctg acacaggttg tcgatgtgcc ggggcaggtg cagccgatgg
     2041 tggtcagccc ggacaaacgt tatctctatg ttggtgttcg ccctgagttt cgcgtcctgg
     2101 cgtatcgtat tgccccggac gatggcgcac tgacctttgc cgcagagtct gcgctgccgg
     2161 gtagtccgac gcatatttcc accgatcacc aggggcagtt tgtctttgta ggttcttaca
     2221 atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct gccagtgggc gtcgtcgatg
     2281 tggtcgaggg gctggacggt tgccattccg ccaatatctc accggacaac cgtacgctgt
     2341 gggttccggc attaaagcag gatcgcattt gcctgtttac ggtcagcgat gatggtcatc
     2401 tggtggcgca ggaccctgcg gaagtgacta ccattgaagg ggccggcccg cgtcatatgg
     2461 tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc tcagtggatg
     2521 tctgggaact gaaagatccg cacggtaata tcgaatgtgt ccagacgctg gatatgatgc
     2581 cggaaaactt ctccgacacc cgttgggcgg ctgatattca tatcaccccg gatggtcgcc
     2641 atttatacgc ctgcgaccgt accgccagcc tgattaccgt tttcagcgtt tcggaagatg
     2701 gcagtgtgtt gagtaaagaa ggcttccagc caacggaaac ccagccgcgc ggcttcaatg
     2761 ttgatcacag cggcaagtat ctgattgccg ccgggcaaaa atctcatctc atctcggtat
     2821 acgaaattgt tggcgagcag ggactactgc atgaaaaagg ccgttatgcg gtcgggcagg
     2881 gaccaatgtg ggtggtggtt aacgcacact aaccgctgat ttaaccggcg cagtctctcc
     2941 tgcgccggtg tattaaccta tctcctgtaa cgcatgtctc tggcgttcga cgatattggt
     3001 ccacaaattg tctttatcgt cagtccacaa attaatcagc aaggcaaaaa agcgttcagc
     3061 tgccggagaa agcacggcat ctttgcggcg aataatcccc aatgtccgac gtataacggg
     3121 ttccaccagt gggataccaa taatcgaaga atagggcgca tggggcattg ccagaccagg
     3181 aagcgccgaa atccccagtc ctgcctccac tagtcctaat gacgtcgaaa gatgacgcac
     3241 ttcgtaaaac cagtccagct tccagggctt gtcggccagc tgttgttcta tcaacagtcg
     3301 gttgccgctg gaggagcgta cgccaatcat tttgtaaccc actaactcct gccattctac
     3361 aagctgcttt ttggccaatg gatggtcacg ccgacaggca gcacgaacgg ctcgttgacc
     3421 agtggggtaa aatcaatgga tgaatttgtg acgttgttca tgtttatgcc aaagtctgat
     3481 tcgttacaca gcaccgattc catgcaatta ttcgtgcctt gttccagaat ccgcacttta
     3541 atattgggat acagctcatt aaatttaccg attgccagcg gtaaaaaata aaatactgcg
     3601 gtcggaatac acgctaacgt caccatacca cgatgataag cattcatatc acgaatatta
     3661 aaaagcgttt catcgaattt ctttattaat tcccgcgcct ccgggagcaa cctttttcct
     3721 gcttttgtta acgtaacctt gcgagttgta cgctcaaata gttgaatatg taaatcttcc
     3781 tccatctttt ttattcttcg cgttaatgca ggttgcgtaa tattgagtaa tttagcggca
     3841 ttgttaaacg aactggactc tgccagtatg acaaatgcct tcatacttga taattcatgc
     3901 ttcattatta ctccggaaaa tggaagcgac gattttgggt ggctggccgt taaaaatttt
     3961 aactgcattt agccaactta aattaatgaa aaaatgttat taatcgttga gctaaagtca
     4021 ttagagatgc tttgccctta atgtaaccat atcgcaataa gggtaatg
//
LOCUS       DAJTSL010000239         4004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTSL010000239 DAJTSL010000000
VERSION     DAJTSL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.242"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            4..516
                     /locus_tag="OO433_003938"
     CDS             4..516
                     /locus_tag="OO433_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6052231.1"
                     /translation="MATQLPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAV
                     IHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIMASNLLFSLGASMTLGGVAQML
                     APKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEG
                     DGGQVVVIGR"
     gene            577..>4004
                     /locus_tag="OO433_003939"
     CDS             577..>4004
                     /locus_tag="OO433_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6052232.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFSVQALVENTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMVWCR
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDAQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSCTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINNTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCSLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASIS"
BASE COUNT          933 a          985 c         1262 g          824 t
ORIGIN      
        1 gcactggcca cacagctccc ggcgtttcgt cagaaactga gtgacggctg gtatcaggtg
       61 cgcattgccg ggcgtgatgc aggtgaaacc gaattgtctg cccgtcttaa tgagccgctg
      121 gcaaatggtg cagtgatcca catagtaccg cgtctggtgg gagctaaaag tggcggtgtg
      181 tttcaggcgg tgctgggggc agctgttatg gcggttgcta tatggatgcc gggggtagga
      241 attatggcga gtaatctgct gttttctctc ggtgccagta tgacgcttgg cggtgttgca
      301 cagatgctgg caccgaaagc cagaactccc cgtacacaga caacggataa cggcaaacag
      361 aacacctgtt tctcctcact ggataacatg gttgcccagg gcaatgttct gcctgttctg
      421 tacggtgaaa tgcgcgtggg gtcgcgggtg gtatctcagg agatcagcac ggcagacgaa
      481 ggggatggtg gtcaggttgt ggtgattggt cgctgatgca aaatgtttca tgtgaaaccg
      541 cctgcgggcg gttttgtcgt ttatggagcg tgaggaatgg gtaaaggcag cagtaagggg
      601 cataccccgc gcgaagcgaa ggacaacctg aaatccacgc agttgctgag tgtgattgat
      661 gccatcagcg aagggccggt tgacggtccg gtggatggat taaaaagcgt gctgctgaac
      721 ggtacgccag tcctggacag cgaggggaag acaaactttt ccggtgttac ggtggtgttc
      781 cgcgccggcg agcaggagca gacaccgccg gaggggtttg aatcttccgg ctcagagact
      841 gtgctgggta cggaagtgaa atacgacacg ccgatcaccc ggaccatcac gtcggcaaac
      901 attgatcgtc tgcgcctgac cttcagtgtg caggcactgg tggaaaacac ctcaaagggg
      961 gaccggaatc cgtcggaagt ccgcctgctg gttcagatac agcgtaacgg tggctgggtg
     1021 acggaaaaag acatcaccat taagggcaaa accacttcgc agtatctggc ctcggtggtg
     1081 gtgggtaacc tgccgccgcg cccgtttaat atccggatgc gcaggatgac accggacagc
     1141 accacagacc agctgcagaa caaaacgctc tggtcgtcgt acaccgaaat catcgatgtg
     1201 aaacagtgct acccgaacac ggcgctggtc ggcgtgcagg tggattcaga gcagttcggt
     1261 aaccagcagg tgagtcgcaa ttatcatctt cgcgggcgca ttctgcaggt gccgtcgaac
     1321 tataacccgc agacgcggca atacagcggt atctgggacg gaacgcttaa gccagcatac
     1381 agcaacaaca tggtctggtg tcggtgggac atgctcactc atccgcgcta cggcatgggg
     1441 aaacgtcttg gtgcggcaga tgtggacaaa tgggcgctgt atgtcatcgg ccagtactgc
     1501 gaccagtcag tgccggacgg atttggcggc acggagccgc gcatcacctg taacgcttac
     1561 ctgaccacac agcgtaaggc gtgggatgtt ctcagtgatt tctgctcggc gatgcgctgt
     1621 atgccggtat ggaacgggca gacgctgacg ttcgtgcagg accgaccgtc ggataaagtg
     1681 tggacctata accgcagtaa tgtggtgatg ccggatgatg gcgcgccgtt ccgctacagc
     1741 ttcagcgccc tgaaggaccg ccataatgtc gttgaggtga actggattga tccggacaac
     1801 ggtcatgaga cggcgacaga gcttgtggag gacgcgcagg ccattctccg ttacggtcgt
     1861 aacgtcacga agatggatgc ctttggctgt accagccggg gacaggcgca ccgcgccggg
     1921 ctgtggctga ttaaaacgga actgctggaa acgcagaccg tggacttcag cgtcggcgca
     1981 gaagggcttc gccatgtacc gggtgatgtc attgaaatct gcgatgatga ttatgccggt
     2041 atcagcaccg gtggtcgcgt actggcggtg aacagccaga cccggacgct gacgctcgac
     2101 cgtgaaatca cgctgccgtc ctcctgtacc acgctgataa gcctggttga cggaaatggc
     2161 aatccggtca gcgtggaggt tcagtccgtc accgacggcg tgaaggtgaa agtgagccgg
     2221 gttcctgacg gcgttgcaga atacagcgtg tgggggctga agctgccgac gctgcgccag
     2281 cgcctgttcc gctgcgtgag tatccgtgag aacgacgacg gcacgtatgc catcaccgcc
     2341 gtgcagcatg tgccggaaaa agaggccatc gtggataacg gggcacactt tgacggtgac
     2401 cggcgcggca cggtgaatgg tgtcacgccg ccagcggtgc agcatctgac cgccgaagtc
     2461 accgcagaca gcggggaata tcaggtgctg gcgcgatggg acacgccgaa ggtggtgaag
     2521 ggtgtgagct tcctgcttcg cctgaccgtg gcagcggacg acggcagtga gcggctggtc
     2581 agcacagcca ggacgacgga aaccacatac cgcttcaggc agctggcgct ggggaattac
     2641 agtctgacag tccgggcggt aaatgcccgg gggcagcagg gcgatccggc gtcggtatcg
     2701 ttccggattg cggcaccggc agcgcctgtc actattgaac tgataccggg gtattttcag
     2761 ataacggcgg tcccgaaact ggctgtatac gacccgacgg tgcagtttga gttctggttt
     2821 tcggaaaagc ggattgcgga tatcaggcag gttgaaacca gcgcccgcta tcttggcacg
     2881 gcgctgtact ggatagccgc cagtatcaat atcaggccgg gccatgatta ttatttttac
     2941 gttcgcagtg tgaacaccgt tggcaaatcg gcatttgtgg aggccgtcgg tcgggcgagc
     3001 gatgatgcgg aaggttatct gagtttttat aaagggttga tcaataacac gcatctcggc
     3061 aaggagttgt ggacgcagat tgataacggt cagcttgcgc cggacctgac tgaaatcagg
     3121 acgtccatta cgaatgtcag caatgaaatc acgcaaaccg tcaataaaaa actggaaaat
     3181 cagagtgctg caatccagca gatacagaaa gttcaggttg atacaaataa taacctgaac
     3241 agcatgtggg ctgtgaagct gcaacagatg aaggacggac gcctttatat tgcgggtatc
     3301 ggtgccggta ttgagaatac gccagcagga atgcagagtc aggtgctgct ggcggcagac
     3361 aggattgcga tgattaatcc tgcgaatggc aacacaaagc cgatgtttgt tggtcagggc
     3421 gatcagatat tcatgaacga cgtgttcctg aaacgcctga cggctccgac cattaccagc
     3481 ggcggtaatc ctccggcatt ttccctgaca cctggcggac ggctgacggc gaaaaatgcc
     3541 gatatcagcg gtaacgtgaa cgcgaactcc gggacgctca acaacgtcac gattaataag
     3601 aactgtcggg ttctgggaaa attgtccgcg aaccagattg aaggcgatct cgttaaaaca
     3661 gtgggcaaac ctttcccacg ggactcccgg gcaccggaga ggtggccatc agggaccatt
     3721 accgtcaggg tttatgacga tcagccgttt gatcggcaaa ttgttattcc cgcggtggcg
     3781 tttcgcggtg ctaaacatga gcggaagaat aacaatattt attcgtcatg cagcctgata
     3841 gtgaagaaaa acggtgctga aatttataac cgaacgaccc tggataatac gctgatatat
     3901 acgggtgtta ttgatatgcc tgccggtcac ggtcacatga cgctggagtt ttctgtatcg
     3961 gcatggctgg taaatggctg gtatcccaca gcaagtatca gcga
//
LOCUS       DAJTSL010000240         3965 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTSL010000240 DAJTSL010000000
VERSION     DAJTSL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.243"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            7..3480
                     /locus_tag="OO433_003940"
     CDS             7..3480
                     /locus_tag="OO433_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6052233.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPESFESSGSETVLGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKCLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTCRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTLGYFQITAVPRLAVYDPTV
                     QFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTD
                     GQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTVTVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSCVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGISIS"
     gene            3548..>3965
                     /locus_tag="OO433_003941"
     CDS             3548..>3965
                     /locus_tag="OO433_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6052234.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEKKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKG"
BASE COUNT          927 a         1022 c         1215 g          801 t
ORIGIN      
        1 gatgtaatgg gcaaaggtgg cggcagggcg cacacaccgc gtgaggcgaa agacaatctc
       61 aaatccacgc agatgatgag cgtgattgat gcgattggtg agggaccgat agaaggcccg
      121 gtgaaaggcc tgcagagtat tctggtgaac aaaaccccgc tgacggacac ggacggtaat
      181 cccgtgatac acggtgtgac cgcggtctgg cgtgccgggg agcaggagca gacaccaccg
      241 gaaagctttg agtcctccgg ctctgaaact gtactgggtg tcgaagtgac cagggcaaaa
      301 ccggtaacac gcaccattac gtcagcgaac attgaccgcc tgcgggtgac cttcggggtg
      361 cagtcactgg tggagaccac gtcaaagggt gaccgtaatc cgtcctctgt ccgtctgctg
      421 attcagttac agcgtaacgg taactgggtg acagaaaagg atgtcaccat taacggcaag
      481 accacctcac agttccttgc gtcggtgatt ctggataatc tgcctccccg cccctttaac
      541 atccggatgg tcagggagac ggcggacagc accacggacc agctgcagaa cagaacgctg
      601 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggccattgtg
      661 gggatgcagg tggatgcgga gcagtttggt ggtcagcaga tgacggtgaa ctaccatatc
      721 cgcggtcgca tcatccaggt gccgtcaaac tatgacccgg aaaaacgcac gtacagtggt
      781 atctgggacg gcagtctgaa accggcatac agcaacaacc cggcctggtg cctgtgggac
      841 atgctgactc acccgcgcta cggcatggga aaatgtctgg gggcggcgga tgtggacaag
      901 tgggcgctgt atgccatcgg gcagtactgc gaccagacgg tcccggatgg tttcgggggg
      961 accgagccgc ggatgacctt taatgcgtac ctggcacaac agcgtaaggc gtgggacgtt
     1021 ctcagtgatt tctgctctgc gatgcgctgt atgccggtat ggaacggtca gacgctgacg
     1081 ttcgttcagg accgcccgtc ggatgtggtg tggccgtaca ccaacagcga tgtggtggtg
     1141 gatgataacg gcgtgggatt ccgctacagc ttcagtgccc tgaaggaccg gcacacggcg
     1201 gtggaggtga attacaccga cccgcagaac ggctggcaga cctccacgga actggtggaa
     1261 gacccggaag ccatactgcg ctacggacgc aacctgctga agatggatgc gtttggctgt
     1321 acctgccgcg gtcaggctca ccgtgcaggt ctgtgggtga taaagaccgg actgctggaa
     1381 acgcagacgg tggacttcac gctcgggtca caggggctgc gtcacacacc gggtgacatt
     1441 attgaaatct gtgataacga ctatgctggg actatgaccg gcggacgtgt cctgtccatt
     1501 gatgctgcca cccgcaccct gacgctggac cgtgaggtta ccctgccgga gacaggtaca
     1561 tcggcggtga acctgattaa cggcagtggt aagccggtga gtgtggacat caccgcacac
     1621 cccgcgccgg accggataca ggtcagtacc ctgcctgatg gtgtggagac atacggggtg
     1681 tggggactct ccctgccgtc actgcgccgt cgcctgttcc gctgtgtctc cgtccgggaa
     1741 aacacggatg gcacctttgc catcacggcg gtgcagcacg taccggaaaa agaagccatc
     1801 gtggataacg gtgcccgctt tgagccgcag tcaggctccc tgaacagcgt catcccaccg
     1861 gcagtgcagc acctgacggt ggaggtgagc gcagctgacg gccagtatct ggcacaggcg
     1921 aaatgggaca cgccgcgggt ggtgaagggg gtgcgcttca gtctgcgact gaccagcgga
     1981 agcggagaag acagccgtct ggtgaccacc gctatcactg cggatacaga gcatcgtttc
     2041 agtggtctgc cgctcgggga atacaccctg acagtcaggg caattaacag ttatggccag
     2101 cagggcgaac cggccaccac caccttccgg attaacgcgc cagcaaaacc cgccaccatt
     2161 gaactgacgc tggggtattt tcagataaca gcggtaccgc gtcttgcggt gtatgacccg
     2221 acggtacagt ttgagttctg gttttcggag gcaaaaatcg cagacacatc tcaggtggaa
     2281 acctctgccc gttatctggg gaccggcagt cagtggagtg tatccggccc gcacattaag
     2341 cccgggaagg atttctggtt ttacgtgcgc agcgtcaacc tggtggggaa atctgctttt
     2401 gtggaggcca gtggacgggc cagcaatgat gcagaagggt atctggggct gtttcgggaa
     2461 aaaataggaa aactgcatct ggctcagggg ctgtgggagc tgatagacaa cagccagctt
     2521 gcggatgaga tggcggagat gaagaccacc atcaccgaaa cccgcaatga aatcacacag
     2581 acggtcagta aaacgctgga ggaccagagc gccaccatac agcagataca gcgcgtgcag
     2641 aaggacacaa atgatgacct tgctgcactt tacatgctga aggtacagaa aacaaaaaat
     2701 ggcataccct atgttgccgg tattggagcg gggattgagg atactgatgg ccagcccctg
     2761 agcaacatac tgctgctggc tgaccgtatt gcgatgatta acccggagga cggcaacacc
     2821 acgccgttat ttgtggcgca ggggaatcag ttgttcatga acgatgtgtt cctgaagcgg
     2881 ctgtttgcgg tgagtatcac ctcgtccggc aatcccccga cgttttccct gacgccggag
     2941 ggcaggctga ccgcaagaaa tgctgatatc agcggtaacg tgaatgcgaa ctccgggacg
     3001 ctcaacaacg tcacgattaa cgagaactgc cgggttctgg gaaaactgtc cgcgaaccag
     3061 attgaaggcg atctcgttaa aacagtgggt aaggcttttc cgcgggactc ccgggcaccg
     3121 gagcggtggc catcagggac cgttaccgtc aggatttatg acgatcagcc ttttgaccgg
     3181 caaattgtta ttccggcggt ggcattcagc ggcgctaagc atgagagaga gcatactgat
     3241 atttactcct catgccgtct gatagtgcgg aaaaacggtg ctgaaattta taaccgtacc
     3301 gcgctggata atacgctgat ttacagttgc gttattgata tgcctgccgg tcacggtcac
     3361 atgacgctgg agttttcggt atcagcatgg ctggtgaata actggtatcc cacagcaagt
     3421 atcagcgatt tgctggttgt ggtgatgaag aaagccaccg caggcatcag tatcagctga
     3481 attttataac ccatatacgg gcgccagaaa tggcgccttt tttattgcag aaaagcgaga
     3541 ggtaattatg cgtaaagttt gtgcagccat tttgtccgca gccatctgcc tgtccgtatc
     3601 cggtgcgcct gcatgggcgt ctgaacatca gtccacactg agcgcggggt atcttcatgc
     3661 ccgtacgaac gctcccggca gcgataatct gaacgggatt aacgtgaaat accgttatga
     3721 gtttacggac gcgctggggc tgattacgtc cttcagttat gccaatgctg aagatgagaa
     3781 aaaaacgcac tacagcgata cccgctggca tgaggattcc gttcgtaacc gctggttcag
     3841 cgtaatggcg gggccgtctg tgcgcgtgaa tgaatggttc agcgcgtatg cgatggcggg
     3901 tgtggcttac agccgtgtgt cgactttctc cggggattat ctccgcgtaa ctgacaacaa
     3961 gggga
//
LOCUS       DAJTSL010000241         3950 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTSL010000241 DAJTSL010000000
VERSION     DAJTSL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.244"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..285
                     /locus_tag="OO433_003942"
                     /pseudo
     CDS             1..285
                     /locus_tag="OO433_003942"
                     /inference="COORDINATES: protein motif:HMM:NF019996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            complement(381..1118)
                     /locus_tag="OO433_003943"
     CDS             complement(381..1118)
                     /locus_tag="OO433_003943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052235.1"
                     /translation="MALPFAADACLVASSFEADADEADDDAELADDAAAVFEDSALVS
                     DAFAFVSDVFAAEADLAAALACSVASPAFVVAVEADDAALSADFPAAVALAEACPALV
                     DAALADDAAAVFEPAAAEALCPAAVSEDLAFVSDVFAAFAEFPAAVAEEAALLALEDA
                     FVSDDFAASFEAAASRAEVAASDAFVVAVDAEVAADCCDAAAFVSDVFAALALVAAAL
                     FEDSAAAALFDASVAFVDAVPALEVAD"
     gene            1076..1288
                     /locus_tag="OO433_003944"
     CDS             1076..1288
                     /locus_tag="OO433_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052236.1"
                     /translation="MRRPDRRQQRRVAPRRHPRRRQRQLAVRRRQLRAKVRRNLQQRA
                     PRQRQNGQRILHPPWRLRMRARRKRG"
     gene            1237..1389
                     /locus_tag="OO433_003945"
                     /pseudo
     CDS             1237..1389
                     /locus_tag="OO433_003945"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1366..1545
                     /locus_tag="OO433_003946"
                     /pseudo
     CDS             1366..1545
                     /locus_tag="OO433_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            2224..2896
                     /locus_tag="OO433_003947"
                     /pseudo
     CDS             2224..2896
                     /locus_tag="OO433_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            3069..3491
                     /locus_tag="OO433_003948"
     CDS             3069..3491
                     /locus_tag="OO433_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6052237.1"
                     /translation="MAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVT
                     WLSPEGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIA
                     TEEETSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(3546..>3950)
                     /locus_tag="OO433_003949"
     CDS             complement(3546..>3950)
                     /locus_tag="OO433_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCR6052238.1"
                     /translation="AASTRAGESKIKHKISHDVFEPYPAALHGQFDSISMFYLLHCLP
                     GNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDS
                     EEGLTHILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT         1061 a         1009 c         1071 g          809 t
ORIGIN      
        1 tctgaaaatc cggatgaagc cgggcgttac agcatggacg ttgagtacgg tcagtacagc
       61 gttattctgt tggtggaagg attcccgccg tcacatgccg ggaccatcac cgtgtatgaa
      121 gattcccgac ccggtacgct gaatgatttt ctcggtgcca tgacggagga tgatgcccgt
      181 ccggaggcac tgcgccgttt tgagctgatg gtggaagagg tggcgcgtaa cgcgtccgtg
      241 gtggcacaga acacggcagc cgcgaagaag tcagccagtg atgccagcac atcagcccgt
      301 gaggcggcaa cccatgcgac tgatgctgca ggctcagcac gcgcagccag cacgtcagcc
      361 ggacaggccg cgtcgtcggc tcagtcagcg acttccagcg caggaacggc atcaacaaag
      421 gccactgaag catcaaaaag tgctgccgct gcagagtcct caaaaagcgc ggcagctacc
      481 agtgccagtg ccgcgaaaac gtcagaaacg aatgctgcag cgtcacaaca atcagcagcc
      541 acttctgcat ccaccgcgac tacgaaagcg tcagaagctg ccacctcagc ccgggatgcg
      601 gcggcttcaa aagaggcggc aaaatcatca gaaacgaacg catcctcgag cgccagtagc
      661 gcagcttcct cggcaacagc ggcaggaaat tccgcgaagg cggcaaaaac gtcagagacg
      721 aacgccaggt cttctgaaac ggcagcggga cagagcgcct cggctgcggc aggctcaaaa
      781 acagcggctg cgtcgtctgc cagtgcagcg tcaacaagtg ccgggcaggc ctcagccagt
      841 gccaccgccg ccggaaaatc ggcagaaagc gccgcatcgt ctgcttcaac agccacaacg
      901 aaggctggcg aagccactga acaggccagc gcagcagcga ggtctgcttc cgcagcgaag
      961 acatccgaaa cgaacgcgaa agcgtcggaa accagcgcag aatcctcaaa aacggctgcc
     1021 gcatcgtcag ccagttcggc gtcgtcatcg gcctcatcgg cgtctgcttc aaaagatgag
     1081 gcgaccagac aggcgtcagc agcgaagggt agcgccacga cggcatccac gaaggcgaca
     1141 gaggcagctg gcagtgcgac ggcggcagct cagagcaaaa gtacggcgga atctgcagca
     1201 acgcgcgccg agacagcggc aaaacgggca gaggatattg catccgccgt ggcgcttgag
     1261 gatgcgagca cgacgaaaaa ggggatagta cagctcagca gtgcgaccaa cagcacttcc
     1321 gagtcactgg cggcaacgcc aaaagccgtt aaggccgcgt atgacctggc taacgggaaa
     1381 tacaccgcac aggatgcaac gacagcacag aaagggataa tccagctaag cagcgcgacc
     1441 aacagcacgt ctgaaacgct ggcggcaacg ccaaaggcag taaaagcagc caatgacaat
     1501 gctgagaaac gtctgcagaa agatcagaac ggtgcggata tccctggcaa agacaccttt
     1561 acgaaaaata ttggtgcctg ccgtgccttc ggtgggtcag taagcacaac aacaggaaac
     1621 tggacgactg cacagtttat cgagtggctg gattctcagg gagcatttaa ccatccatac
     1681 tggatgtgca agggttcctg gtcttatggc aataataaaa tcattactga tactggctgc
     1741 ggtaatattc atctcgccgg agctgtcatt gaagtaatgg ggataaagtc agcgatgacg
     1801 atccacatta ccacaccgac cacctccact ggtggtggaa caactaacgc ccagtttacc
     1861 tatattaatc acggaacaga ttattcacct ggctggcgaa gggactataa ctccagaaat
     1921 aagccaacgg catcagagat cggggcgtta ccgtcaggtg gaacagcagt atcatcagtt
     1981 aatctgtctt caaaaggtcg ggtaaccgcg ctgacagaca atacgcaggg ggcaacaggt
     2041 cttgagttat acgaggtgta taacaacgga tatccaacag cgtatggaaa tatcattcac
     2101 ctgaaaggga tgacagccgt tggcgaaggt gagttactca tcggctggag tggtacaagc
     2161 ggtgctcatg ctccggcatt tattcgttca cgacgggata cgaccgacgc aaactggtcg
     2221 ccgtgggcgc agctttacac ctcggctcat cctcctgcag agttttatcc agtcggtgca
     2281 ccaatcccgt ggccatcaga taccgttccg tctggttatg ccctgatgca ggggcagact
     2341 tttgacaaat ctgcttaccc gaaacttgca gccgcttatc cgtcaggcgt gatccctgat
     2401 atgcgtggct ggacgattaa gggcaagccc gccagtgatc gagccgtatt gtctcaggaa
     2461 caggacggca ttaaatcaca cacccacagc gccagcgtat ccagtacgga tttggggacg
     2521 aaaaccacat cgtcgtttga ttacggcact aaatccacga ataacactgg tgcgcatacc
     2581 catagtgtta gcggtacggc tgcttcagcc ggtgcacata cccattcgat gacatttgtt
     2641 tcaggtggtt ccagtggtgc tccgggaagt ggatcacctg attattctaa atacagtgtt
     2701 aacacttctt ctgcaggcgc tcatacgcac tctgtatcgg gtactgctgc aagcgcaggt
     2761 gcacacgcac atactgtcgg tattggtgct catacgcact ccgttgcgat tggttcacat
     2821 ggacatacca tcaccgttaa cgctgctggt aacgcggaaa acaccgtcaa aaacatcgca
     2881 tttaactata ttgtgaggct tgcataatgg cattcagaat gagtgaacaa ccacggacca
     2941 taaaaattta taatctgctg gccggaacta atgaatttat tggtgaaggt gatgcatata
     3001 ttccgcctca tacaggtctg ccagcaaaca gtactgatat tgcaccgcca gatattccgg
     3061 caggcttcgt ggctgttttc aacagtgatg aggcatcgtg gcatctcgtt gaagaccatc
     3121 ggggtaaaac ggtttatgac gtagcgtcag gggacgcgtt atttatttct gaactcggtc
     3181 cgttaccgga aaatgttacc tggttatcgc cggaagggga gtttcagaag tggaacggca
     3241 cagcctgggt gaaagatgca gaagcagaaa aactgttccg gatccgggag gcggaagaaa
     3301 caaaaaacag cctgatgcag gtagccagtg agcatattgc gccacttcag gatgctgtag
     3361 atctggaaat cgcaacggag gaagaaacct cattgctgga agcctggaaa aaatatcggg
     3421 tgttgctgaa ccgtgttgat acatcaactg cacctgatat tgagtggcct acgaaccctg
     3481 tcagggagta atcattggga ttatgccgca gcacgtctta agcaagaacg tgctgcggtt
     3541 ggatgctatt ttttccctga agcggaaaac attactacag taccttgaac cttggtttta
     3601 acattctcga aatgctctga gagtatatgt gttaagcctt cttcggaatc ttttgtgttt
     3661 gaaaagatgc ctttctgatt gtaaatgcgc atcagttttt gaccgaagct attgtgcaca
     3721 actccatcgc caagaattgt ggctccgtat agagttccat cgtcagttaa ggcctgcgcc
     3781 gcattgcgta ttacacagct ttttgtagat atatttccag gcaggcagtg aagaaggtaa
     3841 aacatggaaa tggaatcaaa ttgaccatgt aacgccgcgg gataaggttc aaaaacatca
     3901 tggctaattt tatgtttaat ttttgattcc ccagcccttg tagatgccgc
//
LOCUS       DAJTSL010000242         3893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTSL010000242 DAJTSL010000000
VERSION     DAJTSL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.245"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            115..618
                     /gene="yeaK"
                     /locus_tag="OO433_003950"
     CDS             115..618
                     /gene="yeaK"
                     /locus_tag="OO433_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6052239.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     VNFRRTA"
     gene            complement(619..723)
                     /locus_tag="OO433_003951"
     CDS             complement(619..723)
                     /locus_tag="OO433_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052240.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            892..1338
                     /locus_tag="OO433_003952"
     CDS             892..1338
                     /locus_tag="OO433_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6052241.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1295..2116)
                     /gene="nimR"
                     /locus_tag="OO433_003953"
     CDS             complement(1295..2116)
                     /gene="nimR"
                     /locus_tag="OO433_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR6052242.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2213..3409
                     /gene="nimT"
                     /locus_tag="OO433_003954"
     CDS             2213..3409
                     /gene="nimT"
                     /locus_tag="OO433_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR6052243.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGSNSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     RSYPVL"
     gene            3449..3796
                     /locus_tag="OO433_003955"
     CDS             3449..3796
                     /locus_tag="OO433_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6052244.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFTTFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3818..>3893)
                     /gene="yoaF"
                     /locus_tag="OO433_003956"
     CDS             complement(3818..>3893)
                     /gene="yoaF"
                     /locus_tag="OO433_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6052245.1"
                     /translation="CKLPGGETIDEWALWRRDHPAGEK"
BASE COUNT          894 a          993 c          976 g         1030 t
ORIGIN      
        1 ttacatgatg cgtataaagc ttccgatgag cgtttatatg tcaataagca gaacaaaaac
       61 agccgttcat gataaccttc tgtggttgtt tgcttgtaat ctcaggagcg tgaaatgact
      121 gaaatggcta aaggaagcgt gacgcatcag cggttaatcg ctttattatc acaagaaggt
      181 gctaacttcc gcgtcgttac ccatgaagcg gtaggtaaat gtgaagcggt gtcagaaatt
      241 cgtggcaccg ctttaggcca gggagctaag gcactggtat gtaaagtcaa aggcaatggc
      301 gtaaatcaac atgttctggc gatcctcgct gccgatcaac aggccgatct gagccaactt
      361 gccagccata taggcggatt acgcgcttcg ttagccagcc ctgctgaagt cgatgaactc
      421 acaggctgtg tcttcggcgc gatcccccct ttcagcttcc atccaaaact caaactggtt
      481 gccgaccctc tactcttcga acgatttgat gaaatcgcct tcaatgcagg gatgctggat
      541 aaatccgtta ttttgaaaac cacggattat ttgcgcattg cgcaaccaga actggtcaat
      601 ttccgccgca ctgcgtaact agcctgtccg ttcaataagc agaacggaca aaaccagcac
      661 aacagcaatg gcaaaaaacg atgacgtgat aatcagtgtc tcgacgaaca tttgatcgtt
      721 catagcatgc ccccgggaga tagctgtttt cacgttattg ttagcgtgca caaatggcag
      781 tttgatgaca gttcgcctga tttttatgca aaaaacgtaa agattttcta cttccttcct
      841 gcagcaagcg taaagtaagc aggcttatta ttttttggca aggaaaccac gatgtttgat
      901 gtcactttgc tgatcctgct cgggttagct gcgctgggct ttatcagtca taacaccact
      961 gtcgccgttt caattctggt gttgatcatt gtccgcgtca caccgttaag cacctttttc
     1021 ccgtggattg aaaaacaggg gctaagtatc gggataatca ttctgacgat tggcgttatg
     1081 gcccccatcg ccagcgggac gctaccgccc tcaaccttga ttcactcgtt tctgaactgg
     1141 aaatcgctgg tggcaattgc agtaggcgtg attgtctcct ggctgggtgg gcgcggcgtg
     1201 accttaatgg gcagccagcc gcaactggtc gccgggctgc tggtaggcac tgttttaggc
     1261 gtggcgctgt ttcgcggcgt accggtcggc ccgcttattg ccgcgggtct ggtttcgctg
     1321 attgtgggga aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc cccaggcctt
     1381 tcttaaacat agtaataaag gccgttgttg aatcataccc cagcgtatga gcaactttct
     1441 gtacagtatc gcctttcact aatccctgaa gtgccataat cagctgtaac tgctgacgcc
     1501 actggcgaaa actcaacccc gtctctttta cgatcagtcg cgcaagattg cgttcactca
     1561 tcgcaaaaaa gccagcccat tgccctaatg acccccactc gacgggccct ttcgccatca
     1621 tctccaccat cgtgcggatt ttaggatgag aagaaacggg caggtgcagc tgttgttgtg
     1681 gctgctgagg gagttcatca aacaggacct gtattaatct gcgggtcatc ggctcagcac
     1741 gctgagtcgt cgtcctgttt gccagggtta aaatcaattc ccggcataag ggcgatattt
     1801 tcaatgtgca acatgttgtc ggcattgtca ctgcgctggg ttcgataaac agaaaacaga
     1861 gttcagcgtt agctgtcact tgattgctat gttccacccc gcccggtatc caaaccgcat
     1921 attgcggcgg caccatccac aaagcatttt ccaccgtaca ggtaattgca ccatgtaatg
     1981 ctagtatcag ctgtcctttg cggtgctgat gtacaggact ggttaactca tccgttccgg
     2041 cgtgaataca aaatgcgacc gcggcttcat ggtgtcggtc gggttcatag ccattgagat
     2101 tcaacctgtg catcattttg tccgaattta gcgataattt gtcattttag cttgattcaa
     2161 cataacaata aaaacggtaa ggtacagcct cgtttgtaac aatgagaagc atatgacctg
     2221 ttcaacttca ttaagcggca aaaacaggat tgtccttatc gctggcattc tgatgattgc
     2281 cacaacatta cgcgtcacct ttaccggcgc agcaccgtta ctggatacga ttcgttccgc
     2341 ttactcgctg acgacagcgc aaaccggctt attgaccacc ctgccattat tggcctttgc
     2401 gctaatctca cctttggccg ccccggtagc gcgacgtttt ggtatggaac gtagcctgtt
     2461 tgccgcgtta cttttgatct gtgctggtat cgcaattcgc tctctccctt cgccttactt
     2521 attatttggc ggtacagcgg tcattggcgg tgggattgca ttaggcaatg tcttactgcc
     2581 aggattaatt aaacgcgatt tccctcattc cgtcgccaga cttaccggcg catattccct
     2641 gacaatggga gctgcggcgg cacttggatc ggctatggta gtgccgctgg ctttgaacgg
     2701 ttttggctgg caaggcgcgt tgctcatgct gatgtgtttt cctctgctgg ctcttttttt
     2761 atggctgcca cagtggcgaa gccaacaaca tgcaaatttg agtacctcgc gcgccttaca
     2821 tactcggggt atctggcgtt cgccgcttgc ctggcaggtc acattgtttc ttgggagcaa
     2881 ctcactggtc tattacgtga ttattggctg gcttccggcg atcctcatca gtcacggcta
     2941 tagcgaagca caggcgggtt cactgcatgg tttgctgcaa ctagccacag cagctcccgg
     3001 tttgctgatc cctcttttct tacatcatgt gaaagaccag cgtggtattg cagcgttcgt
     3061 tgccttgatg tgcgcagtgg gcgctgttgg gctctgcttt atgccagcgc acgcgatcac
     3121 ctggactctg cttttcggtt ttggttccgg cgcaacaatg atactggggt tgacgttcat
     3181 tggtcttcgg gcaagttctg cgcatcaggc ggcggcactc tcggggatgg cacaatccgt
     3241 cgggtatttg ctggcagcct gtgggccgcc gctaatgggt aaaatacacg atgctaacgg
     3301 taactggtcc gtaccactga tgggtgttgc aatactttca ctactgatgg cgattttcgg
     3361 actttgcgcc gggagagaca aagaaattcg ctcatatccg gtgctatagt gacgtgacaa
     3421 atcatgcgtg aaagggagaa caaacacgat gaatattcag tgcaaacgcg tttatgatcc
     3481 ggctgaacag agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa
     3541 aaaaaccgat ttagcccttg atgagtggga taaagaaatc acgccgtcaa cggaactgcg
     3601 caaagccttt cacggcgaag tcgtcgattt tacaaccttt cgcgagcaat atcttgcaga
     3661 actggcgcaa cacgagcaag aaggaaagcg gctggcggac atcgccaaaa aacagccgct
     3721 gaccctgctc tacgcagcaa aaaacaccac gcagaaccat gcgctggtgc tggccgactg
     3781 gctacgtagc ttgtgatttt agtacagcat ccggcggtta tttttcacca gccggatggt
     3841 cccgtcgcca caatgcccat tcatcaatcg tttcaccgcc cggtaatttg caa
//
LOCUS       DAJTSL010000243         3869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTSL010000243 DAJTSL010000000
VERSION     DAJTSL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.246"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..720
                     /gene="pgrR"
                     /locus_tag="OO433_003957"
     CDS             <1..720
                     /gene="pgrR"
                     /locus_tag="OO433_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6052246.1"
                     /translation="TEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVEHA
                     AKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRIGP
                     DIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVRME
                     GQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYYPH
                     RRHAGSAFSLFIDRLKYKGAV"
     gene            1057..2670
                     /gene="mppA"
                     /locus_tag="OO433_003958"
     CDS             1057..2670
                     /gene="mppA"
                     /locus_tag="OO433_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6052247.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMKQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRIMYIVKH"
     gene            complement(2721..3752)
                     /gene="ynaI"
                     /locus_tag="OO433_003959"
     CDS             complement(2721..3752)
                     /gene="ynaI"
                     /locus_tag="OO433_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6052248.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1093 a          850 c          897 g         1029 t
ORIGIN      
        1 actgaagcgg gcgagcatct tttgtctgtt cttggcccta tgttgcatga catagattca
       61 gccctggcat ccctgagcga gctgcagaac cgcccatccg ggacaatacg tattactact
      121 gtagaacatg cagcaaaaac gatattgtta ccagcaatgc gcacattcct gaaatcgcat
      181 cctgaaattg atattcagct caccattgat tatggtttga ccgatgtcgt ttctgaacgt
      241 tttgatgcag gcgtccgtct gggtggggag atggataaag atatgatcgc cattcgaatc
      301 gggccagata taccaatggc tattgttggc tcaccggatt atttttctcg ccgaagtgtt
      361 ccaacgtcag tgtcacaatt aatagatcat caggcaatta atttgtatct tcccacatcg
      421 ggtacagcaa atcgctggag attaatacgc ggtggacgtg aagttcgtgt tcgcatggaa
      481 ggtcagcttt tactgaatac gatagacctg atcattgatg ctgcaattga tgggcatgga
      541 ttggcgtatc taccttatga tcaggttgag cgggctatta aagaaaaaaa actgatacgt
      601 gttttggata aattcacacc agatttaccc ggttatcacc tgtactatcc acaccgtcga
      661 catgctggct cggcattctc attatttata gataggctga agtataaagg tgctgtttag
      721 cactacttgc tgatacatta atttaatttc tctcttaacg tattctcagt tcctttcaac
      781 gttttggtta ttttttattc ttcgtacaat ggcgacagat gctgattttg ataccgaaaa
      841 ctggtttgaa cgtgcgaagc ctgaacgtag tattcggtgt tctatgtgct ttgccgcatt
      901 ttggcgtcga ttatttacac taaatctgat ttaatatatt gatacataaa acattttatg
      961 cttcctgtgt cacttttttg atggaagttg tttgcatttc tttaaggcga aacaaataat
     1021 tacgcatcaa ttttaatgtc ggttagaggg aaacttatga agcactctgt ttcagtcacg
     1081 tgttgtgcgc tgttggtcag cagcatttct ctttcgtatg ctgcagaagt tccgagcggc
     1141 acagtactgg cagagaagca ggagctggtg cgccacatta aagatgagcc tgcgtcgctg
     1201 gatcccgcta aagccgtggg cctgccagag attcaggtca ttcgcgatct gtttgaagga
     1261 ctggtgaatc agaacgagaa aggggagatt gtccccggcg ttgcgactca gtggaaaagt
     1321 aatgacaacc gtatctggac ttttaccctg cgcgataacg caaaatgggc ggatggcaca
     1381 ccggtaacgg cgcaagattt tgtctacagc tggcagcgtc tggtggaccc gaaaacgcta
     1441 tcgccatttg catggtttgc cgcgctggcg ggaatcaaca acgcacaggc gattattgat
     1501 ggtaaagcta cgcctgacca gcttggcgtc accgcagttg atgcccatac cttgaaaatt
     1561 cagcttgata aaccgttgcc gtggtttgtg aatttaaccg ctaactttgc cttcttcccg
     1621 gtgcaaaaag ccaacgtaga aagcggtaaa gagtggacga aacccggaaa tctgatcggc
     1681 aatggcgctt atgttcttaa agaacgcgta gtcaatgaaa aactggtcgt ggtaccgaat
     1741 acccattatt gggataacgc caaaacggta ctgcaaaaag tgaccttcct gccaattaat
     1801 caggaatccg cagccactaa gcgttacctc gcgggggata ttgatatcac cgaatccttc
     1861 ccgaaaaata tgtatcagaa gctgttgaag gatattccgg ggcaggttta tacgccgccg
     1921 cagctcggga cctattatta tgcgtttaac acgcaaaaag ggccgacggc agatcagcgc
     1981 gttcgcctgg cattaagtat gacgatagat cgccgcctga tgaccgaaaa agtgttggga
     2041 acgggcgaaa agccagcgtg gcattttaca ccagatgtta ccgccggatt tacgccggaa
     2101 ccttcgccgt ttgaacaaat gagtcaggaa gaactgaatg cgcaggcaaa aactttgttg
     2161 agcgcagctg gttatggtcc gcaaaaaccg ctgaagctga cgcttttgta taacacttca
     2221 gaaaaccatc aaaaaattgc gattgctgta gcatcgatgt ggaaaaagaa ccttggcgta
     2281 gatgttaaat tgcaaaatca ggaatggaaa acctatatcg atagccgtaa caccggcaat
     2341 tttgatgtta tccgcgcctc atgggtgggg gattataatg aaccctccac tttcctgaca
     2401 ttattaacgt caacgcattc aggaaatatt tcacgcttta acaatccggc atacgacaaa
     2461 gttctggccc aggcatcgac ggaaaatacc gttaaagcgc gtaatgccga ttacaacgcg
     2521 gcagaaaaaa tcctcatgaa gcaagcaccg attgcaccaa tttatcaata taccaatgga
     2581 cgattaatca agccgtggct gaaaggttat cctattaata acccggaaga tgtggcgtac
     2641 agccggatta tgtatattgt gaagcattga tgtgatggga actggcgtta cccttgtgga
     2701 taacgccagt gatgtctgat ttagcgcccc tgttccggcg gtgtaatgtt atccatatac
     2761 agcgtctggc tggggaaggc aaagtccgcg ccgtgtgact gcacaatatc gataatcttc
     2821 aaataaacgt cttgttgtgc agcaagccat tcagcccata ccgtggtttt ggtaaagcaa
     2881 taaaccataa tattcaatga cgagtcggca aactggttga aataaaccag taaggtttgt
     2941 cgctggtcga tggccgggtg atttttcagc atctcacgta cggcttcgac aataacgccc
     3001 acttttgccg catcctcata acgtaaacca atggtcgtgg taatgcggcg gttggtcatt
     3061 cgtcctgggt tttctacgct gatcgacgaa aacagcgagt tcggtacgta caatggacga
     3121 ttatcaaagg tcgtaatttt ggtaattcgc cagccaattt ccgctactgt accttcgata
     3181 tttctgtccg gtgaacggat ccagtcgcca atactgaaag gacggtcgaa atagagcatg
     3241 atcccggaaa agaaattact cagaatatct ttaccggcca taccgacagc cagaccacca
     3301 ataccaccaa aggtcagcaa gccagaaagg ctcatgccga aatgttcgcc ataaagcaga
     3361 acaagcacca caataatggt gattttgata atgcgcgaca taatccgcgc actggtgata
     3421 tcgcgacctt ttttaatctg ctgtttttca aactgattaa tcagcaaaaa tagcttaatc
     3481 gtcagaataa ccgcaatcag ggacgtacag ataaaatcga taacgcctgg ggtgataaat
     3541 ttgagtttat agttttctat aacataatta ataatgctac caacagcact gataattatg
     3601 gtgtagatta aaaattgcac cgcatggaat aaaaaacctt ttcttttacg atttccgcgg
     3661 cgaaaccaaa agctcatcag aatcaatgct gcgcagctac cgaaaataat gaccagatta
     3721 agcgcattat ttgtaaacag ttcagcgatc attgttttat caggctcctc cagataattg
     3781 tcgtcatgcc ggaaccccct ggcggggcta ttttaccgcg acaattcatt cagatcatca
     3841 atagtcaggg aaggaagtag caacattag
//
LOCUS       DAJTSL010000244         3858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTSL010000244 DAJTSL010000000
VERSION     DAJTSL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.247"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(43..213)
                     /locus_tag="OO433_003960"
                     /pseudo
     CDS             complement(43..213)
                     /locus_tag="OO433_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OO433_003961"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OO433_003961"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6052249.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OO433_003962"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OO433_003962"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6052250.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDSQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OO433_003963"
     CDS             2894..3634
                     /locus_tag="OO433_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6052251.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEIAATL"
     gene            3669..>3858
                     /gene="ruvC"
                     /locus_tag="OO433_003964"
     CDS             3669..>3858
                     /gene="ruvC"
                     /locus_tag="OO433_003964"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6052252.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDY"
BASE COUNT          955 a          954 c         1077 g          872 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagat cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggctaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggtcacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaatcga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgtccgga aatggctcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgagttta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgcgatg
     1741 ggcgcactgc gcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttattgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gcagagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 attgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgacagtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcatgc
     3241 attcagcaaa tgtggcggta acctcggtac agatggttcc gtagcctatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga tgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacctatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg caccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgagattg cagcgactct ctgatgaggc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctatggcg
     3721 tcatccgcca ggtaggcagg caactgtcct acctgggtag cggatgcata cgcaccaagg
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc agattatt
//
LOCUS       DAJTSL010000245         3837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTSL010000245 DAJTSL010000000
VERSION     DAJTSL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.248"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(521..712)
                     /locus_tag="OO433_003965"
     CDS             complement(521..712)
                     /locus_tag="OO433_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6052253.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            780..1103
                     /locus_tag="OO433_003966"
     CDS             780..1103
                     /locus_tag="OO433_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6052254.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            1066..1809
                     /locus_tag="OO433_003967"
                     /pseudo
     CDS             1066..1809
                     /locus_tag="OO433_003967"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1781..2341
                     /locus_tag="OO433_003968"
     CDS             1781..2341
                     /locus_tag="OO433_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6052255.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENILNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            2348..2581
                     /locus_tag="OO433_003969"
     CDS             2348..2581
                     /locus_tag="OO433_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6052256.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLVEQVDHLSRLFITNSR"
     gene            complement(3051..3518)
                     /locus_tag="OO433_003970"
     CDS             complement(3051..3518)
                     /locus_tag="OO433_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052257.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     AFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          912 a          877 c         1146 g          902 t
ORIGIN      
        1 accaggggga aacgcctggt atctttaagt cctgtcgggt ttcgccacct ctgacttgag
       61 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc caccggcgcg
      121 gcctttttcg ggctcttgtt ttttgctcac atgtttcttt tggctttatc ccctgattct
      181 gtggataact gtgttaccgt ctttgagtgc gctgataccg ctcgccgcag ccgaacgacc
      241 gagcgtagcg agtcagtgag cgaggaagcg gaagagcgcc ggaagctaca ttttctcctt
      301 acgcatctgt gcggcatttc acacccggca tggtgcactt ttcatacaat ccgcactgat
      361 gccgcataag taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
      421 cccgacaccc gctaaaacct gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      481 ctcacaggcc agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccatcat
      541 ccccgaagcg tgcgaggcag ctgcggtaaa gctcgtcagc gtggtcgtga agcgcttcgc
      601 agatgtcagc ctggtcgtcg gcgtccagtt cattcagttt ttccagcagg gtcagcgtct
      661 ggcttctgat gaagcgggcc atgttaaggg cggttttttc ctgtttagtc acagttctgt
      721 ttctccatgt gtggggagtc tgtcggaggt ttttaatgat accggtgaaa cgcgaaagga
      781 tgctcacgat acgggtcacg gatgaggaac atgcgcggct gctggcacgt tgtgagggaa
      841 aacaacttgc gtcatggatg cgtaaggtct gcctgggggc accgccatcg aaaacgtcag
      901 gtcttccgac actggcaccg ccgctgttgc gccagttcgc ttcggtcggc aataacctga
      961 atcagatagc ccggaagata aacagcggac agtggtcggg gcatgaccgg gttcatgtgg
     1021 tggctgcact gatggccatc ggacgtgaac tgagtgagct gagggatgag gtccgtaaac
     1081 agggagagcg ggatgatagt taagtttcat gccaggggaa aaggcggtgg cagtggtccg
     1141 gttgattacc tgctgggccg ggagcgtaac cgggaagggg cgagagttct gcggggtgct
     1201 cccgaagagg tgcgggaact catcgatgcc acgccgtttg cgaaaaaata cacctcgggg
     1261 gttctgtctt ttgcagagca gaccctgcca ccgggagagc gtgaaagggt gatggagagc
     1321 tttgagcggg tactgatgcc cggtcttgaa aaaaatcagt acagcatcct gtgggtggag
     1381 caccaggaca aggggcgtct tgagctgaat tttgtcatcc cgaatatgga gctggcgagc
     1441 ggaaaacggc tccagcccta ttacgaccga gcggaccgtc cgcgaatcaa tgcctggcag
     1501 acactggtga atcaccatta cgggctgcac gacccgaacg caccggagaa caggcggaca
     1561 cttgtcaccc cgaataatct gccgaaagcg aaacaggagg ccgcagaagc gattacgagg
     1621 ggactggagg ctctttacca tgccggagcg ctaaaaacgc gtcaggatgt cactgaggcg
     1681 cttacagcgg caggttttga ggttgtgagg acaaccagga gcagtatcag tatcgccgac
     1741 ccggagggcg ggcgtaatct caggctgaaa ggggcgctgt atgagcaatc ttttacaaat
     1801 ggggacagac ttcgagaaga aactgaaaga gcgtgccgtc tctacagaga acatactgaa
     1861 cagcgagttc aggaagctcg aagcgtctgt agacagggaa ctgaacttaa acgggcagaa
     1921 aatcagagac gctatcagcg cacacacagc agcggtgaaa gaacaactgg agacgctgaa
     1981 cagtgccgtg aacgcgcagt tcagcgccac agagaacgaa ctggcccggc ggcaggaaga
     2041 gctgctctgg aagctggtca aagggcggat actgtacccg tcgctgacgg cgctttgcgt
     2101 cacgggcggg atttttctgg cgtcatgggg gctgattcag tggcaggaaa gccggattgc
     2161 ggcgaacatc ctggctatca gggaccagga agagacgctg gcgaaactcc gggaaaaaac
     2221 atggggtgtg acgtatcacg agggcaggaa cgggaaattt ctggtattgc cgtccggggt
     2281 gaagggggaa aacaactgga cggtggagaa gaagaacgca gtaagactgg tcagggagta
     2341 agtgagtatg acagagctgg aaatgcactt actgaacgcc ttagagcaat tgcagcagga
     2401 ctatacgcag cggctgaaag actgggagag cgccttcacg gatttgcagc atatgttcga
     2461 gctgacacga caggagaacg ggcaactgag ggaacagtgc gacgacttga gtcagcaggt
     2521 gcagagatta gtcgagcagg tggaccactt gagtcggtta tttatcacga acagcagata
     2581 agggatgaac aggaaaggca ggcacgcctg gaacgggagc gtgcgcggtc ctggcatgaa
     2641 gagcaggaac gggaagcacg ggtatggcgt ggcccgtcaa tagggatatg acggtgagtt
     2701 gttccgggag gttgccagat ggtaccggaa cgcgttaaaa acgcatccgg gcgcgattct
     2761 ggcgtgtcgt ttttgttgct gtgttttgcc tctttgccag atcgcgctca ttcctcagcc
     2821 tcatcccgcg atgtctgcgt atgtcatcaa ctgaaaagat ggaaaaaaaa cagtgtcatg
     2881 aggtttacgc aggcaaaacc agcgttattc acatggctga atagctatgc atacggttaa
     2941 aatttatcag gtgcgatcgc ggcagctttt cgggtggttt gttgctattt ttacctgtca
     3001 gcagccgtga tcgcgctgaa cgcatttcag cggtgcgtac aattaagata ttacgttaaa
     3061 ttgtgaggga atggtcccca tatactgact ttatcatagg gatagggttg gtagtgggtc
     3121 gattgggttt cggcattgtt tccgttaatg ccaattgcat ttttgtattt gatgcaacag
     3181 aaggcgtgag attctcctgg gcctggaaag gctaagtgtt tagaattgat ggcaaaatat
     3241 cttccgtccg atttttgaag tagttgttgt ttggctgtct cccaaagggt gctgttacaa
     3301 acttcttgaa tttgtagttt ttgcagggct cttattactg cgttgtcatg ttccaattct
     3361 ttaggggagt gtattattct ctcttttaat aagctgttta ccacagggtt tagttgttgt
     3421 ctaaagcacg cagataatgc cattgctaca caattttgtt cccgaggaag catgtttggc
     3481 ggggtatgcc gtgtttgatg tgatatgtct attctcatag aaggtctctg gcttatagca
     3541 tcaacaaata cggctataaa acaaaatttt catagagtga ttcaagatct tcttgagatc
     3601 cttttttgcg gcgcgtaatc tgctgcctgc aaacaaaaaa accaccgcta ccaacggtgg
     3661 tttgtttgcc ggaatctaga gctaccaact ctttcttccg aaggtaactg gcttggtgga
     3721 gcacagatac caaatactgt ccttctagtg tagccgcagt taggccacca cttcaagaac
     3781 tcttaatatc tcaatccacc ttgtctagtt accaatggct gctgccagtg gcgttaa
//
LOCUS       DAJTSL010000246         3831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTSL010000246 DAJTSL010000000
VERSION     DAJTSL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.249"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(589..1536)
                     /gene="icsP"
                     /locus_tag="OO433_003971"
     CDS             complement(589..1536)
                     /gene="icsP"
                     /locus_tag="OO433_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6052258.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGTHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2917..3069)
                     /locus_tag="OO433_003972"
                     /pseudo
     CDS             complement(2917..3069)
                     /locus_tag="OO433_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3125..3817
                     /gene="ospZ"
                     /locus_tag="OO433_003973"
     CDS             3125..3817
                     /gene="ospZ"
                     /locus_tag="OO433_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR6052259.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASIPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RFSQIKARMSVT"
BASE COUNT         1194 a          719 c          700 g         1218 t
ORIGIN      
        1 aatgataaca ggtgtctgga aatatagggg caaagccacc cacgaacgat ccagcatata
       61 atcaagaaat tagatttcat ttaacataag atagattatg tgcaccaaga ggcatttcct
      121 taatttctga atttacaggc tcttttgctc tctttgctat gctgtggatt tggaaagata
      181 gagtgctggc tgattattta ggtattttca tcagcatgtc cctgatagca ctgttccatc
      241 agcgctttca ggcgataata gtagaccttg ggtagcctct gctcatgtgt aacttgatac
      301 cccgagaaca gccacgatga tctaagcggt tcttatttct ccggtctcag ggtcggtaat
      361 gccaacagta agtccacagt aatcaacgaa gagttttttg aaccgccccg gaaatcctgg
      421 agactaaacg ccctgagaaa gaggtaaaca gaatgactaa aaatacacgt ttttcccccg
      481 aagtccgtca acgggcagtt cgtatggttc tggaaagtca gggcgaatat gactcacaat
      541 gggcggcaat ttttagccat gaaaacgtaa tcaactcggt gaaccaattc aaaaaatata
      601 ctttatacct gcggaagtga gaaaaccaat atattcaata cttgcactat ttttaatggt
      661 gccagatata ttggtacttt gctcataaag agatgtatca ccttttttat tagaaatgta
      721 atttctggag ccttctatat aaaattttgc actgggggtg acatagtatc caatattcgc
      781 agcaacagag cagtaatttt ggttttttat ttcatcttta aaaattgtct cagtctgata
      841 gtgtttatct gtatcagatg aacgaaccca accactatat ttcagttctg ctccaaactc
      901 aaaattctca tggcggtaat tagcagttaa tccaatataa ggtattttga agagctgttt
      961 gtaacctatt gttctttcac cactaggatg tgtccctttt ttattcctgt tccccccgtt
     1021 ttcactataa atataactcc ctcccattgc attaaaactg taacgactct cttggtaacc
     1081 tgctattagt ccaagtcgat aatccaaatt atttaataac caacctttaa gattcagctc
     1141 gaattcgtta gcatcacgga gatgcgtatt gggatgccag ctctggtcgg tccatacctg
     1201 aggggtattg gaattgttcc agtctttatc aaccatgctt gctttctggt tacctaaagt
     1261 agtccaaccg gaaactccga aagacacttt tggaattagc ttccaatcga tgccacctct
     1321 aacaatagtc gcattactgt atttccagtc cagttgacta atttttctcc ccccttcctt
     1381 gggatgataa acgcgttctt ttgttttgcc actcagagat cctagactaa tatccgtact
     1441 gatgttatcc ggtatgaaaa gtggatagtt agtggtagca tgtgtagtaa agatcgcagg
     1501 tacacaaagt gcaagtacaa ggaattttaa tttcatcgag aactctactt ttttggttga
     1561 aatgtccatg atcttcttac tttataagat aaacgtcaat gccaagcatt attttaatta
     1621 aatatttgtt tatgttacca agaacatatc ttttattata atatttattg aacttttgta
     1681 aaaataaaaa aatacctcat tgtgcgaata aagtaacggg ggccatagat ttctctacct
     1741 accttttggt gttcatacct gaggctgata gtttcaggat tccgcgacaa ttctactcac
     1801 cttcttgttc tctcgccaat atgaaatcaa tcagaaaagc gttccataag aacaacgata
     1861 agacaataaa ttgtcatcac ggctgcaata gccaacgtgc ctttcagtac caacatgaca
     1921 acctcctgta tttgacgtaa cgtgtgctga taaatatgta tttgtatctc aactgactca
     1981 atacaacact taagtatagt ttttcagatt ttgtttttcc cgtgaaatat tatggaaatg
     2041 gcagaagcaa tacattgaat tccgattcga gtgaatacgg ctagcgggca cctcacttta
     2101 gcactgaagc catcaacttt tatttgactt atttcaatgg aaagctttct cactatcagt
     2161 acaggtaatt cccatccttt taaagtcgag agtcagactg cgactaatac agttaaagtg
     2221 accaaactca gttatcctct acacatttta cctcatgttt acttggacaa ttcattggtg
     2281 cttttataaa agaataaata ttctactaat ttaatgcatc acctaaaata gtatttttta
     2341 taatagaact gtatctatag tatattatta aagcttaatt atttattatg ctttaatgtg
     2401 gttgtccgat taaggactaa tccttgaaaa cgccgtatag ggcgggagtt cactctattg
     2461 cgataacgta accgtacaat attgtgcgtt tcccaaattg accactactg aactattctg
     2521 ggatttaaat gcaataaaat ggttggttga aggtcgtggt gatgatgtta gtccttttga
     2581 gaatattcca gtgccagatc ttacttataa taaagaggct tggcagtttg gataagctat
     2641 tgtccttatt ccactaatac aaactttttg taaagtatat taaatatact tcatttcata
     2701 ctgaaatccc cggaagtatt caatcaaaca attacaccat aaaaggaaga gggagcccca
     2761 attaagttat caagcatgtt tatgatacct gcagggtata aattttctgg ctgcaagcag
     2821 acacatataa aacgtcttta ttgatcctac ccacataata tggacgcagt cgtaagtgag
     2881 gctctgtgag cctacccacg taatatggac acaggcctaa gcgaggttct ggttttcaaa
     2941 ttgttccgga ctgaggccac cacaccaacg gtgccgtcgc cagcgattgt aatcgcactc
     3001 gatataatta aacactgttg tccgcactat ttcccgatag ataaagtgtt ctccgtggag
     3061 acattccact taggaacaat tatattgttc atggtaatgt ttatatttga gtatagagat
     3121 taaaatgatt agtcccatca agaatattaa gaacgtattt ccaataaata ctgcaaatac
     3181 tgaatatata gtgagaaata tttatcctag agttgaacat ggttacttta atgaatcacc
     3241 aaacatatat gacaagaagt atatctctgg tataaccaga agtatggcac aattaaaaat
     3301 agaggagttt attaatgaga aatccagacg acttaattat atgaagacaa tgtattctcc
     3361 atgtccggaa gattttcagc ctatttccag agacgaagcc agtataccgg aaggaagctg
     3421 gttgacagtt atatctggaa aacgtcctat ggggcaattt tctgtggata gtttgtacca
     3481 tcctgactta catgcattat gtgagctccc agagattagt tgtaagatct tccctaaaga
     3541 aaatagtgat tttttgtata taattgttgt attcagaaat gattctcccc aaggagaact
     3601 acgggcaaat cggtttatag aactatatga tataaaaaga gagattatgc aagtgttaag
     3661 agatgagtcg ccagagttaa agtctattaa atctgagatt attattgctc gtgaaatggg
     3721 agaacttttt agttatgcat cagaggaaat agatagctac ataaaacaaa tgaatgacag
     3781 gttttctcaa atcaaggctc gaatgtctgt tacttaatgt atattaattg t
//
LOCUS       DAJTSL010000247         3819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTSL010000247 DAJTSL010000000
VERSION     DAJTSL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.250"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..212
                     /locus_tag="OO433_003974"
                     /pseudo
     CDS             1..212
                     /locus_tag="OO433_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
     gene            194..1576
                     /gene="torZ"
                     /locus_tag="OO433_003975"
                     /pseudo
     CDS             194..1576
                     /gene="torZ"
                     /locus_tag="OO433_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(1741..2712)
                     /gene="cmoB"
                     /locus_tag="OO433_003976"
     CDS             complement(1741..2712)
                     /gene="cmoB"
                     /locus_tag="OO433_003976"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6052260.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2709..3452)
                     /gene="cmoA"
                     /locus_tag="OO433_003977"
     CDS             complement(2709..3452)
                     /gene="cmoA"
                     /locus_tag="OO433_003977"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6052261.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MISMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(3493..>3819)
                     /locus_tag="OO433_003978"
     CDS             complement(3493..>3819)
                     /locus_tag="OO433_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6052262.1"
                     /translation="LRLQYRVAYGDGGFSELQSAIRIHGNAVEYIPIAIVLMLFMEMN
                     GAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRSGMSATWCALLLMVLANLWYMPWE
                     LVFSLR"
BASE COUNT         1010 a          952 c         1009 g          848 t
ORIGIN      
        1 taggcgagct ggctaccgtg aatcctggta ccgatctcac cgtcgttgat gccagtggca
       61 aacagccgat cgttctgttg cagggttatc aaatgcaggg cagtgaaaac acgctctacc
      121 tggcggcagg tcaacggctg gcgctagcca cattaagtga agaaggtatc aaggcgctca
      181 cgctaaacgg ggaatggcag gctggggaat tcagcgccag caatatggtg aacagaaaca
      241 ctggatgctg gtgacgctgg cggcggtgtt agggcagatt ggtacaccgg gcggcggttt
      301 tggtttctct tatcattact ccaatggtgg caacccgaca cgtgttggcg gcgtgctgcc
      361 ggaaatgtct gcggcgattg ccggacaggc cagcgaggcc gctgatgatg gcggaatgag
      421 ggcgattcct gttgcccgta tcgtcgatgc gctggaaaat cctggcggca aatatcagca
      481 taacggtaaa gagcaaacgt atccgaatat caaaatgatc tggtgggcgg gtggcggtaa
      541 ctttacccat caccaggata ctaatcgtct gattaaagca tggcagaaac cggagatgat
      601 cgtcgtttct gaatgctact ggaccgcagc tgctaaacat gcagatatcg tattaccgat
      661 caccacatcg tttgagcgca atgacttgac gatgaccggt gattacagca accagcatat
      721 tgtgccgatg aagcaggctg tcgctccgca atttgaagcg cgaaacgatt ttgatgtgtt
      781 tgccgatctt gcggaattac tgaaacctgg cggaaaagag atctataccg aaggtaaaga
      841 tgaaatggcg tggctgaaat ttttctatga tgccgctcag aaaggtgccc gtgcgcaacg
      901 cgtgactatg ccgatgttta atgccttctg gcagcaaaat aaactgatcg aaatgcgccg
      961 cagcgagaag aacgaacagt acgttcgtta tgctgatttc cgcgccgatc cggtgaaaaa
     1021 tgcgctgggt acgccaagcg gcaaaattga gatttactcc aaaacgctgg aaaaatttgg
     1081 ctataaggat tgcccggcac atccaacctg gcttgcgcct gatgagtgga agggtaccgc
     1141 cgacgagaag cagttgcagc ttctgaccgc acatccggca caccgtttac atagtcagct
     1201 taactatgcg gaactgcgta aaaaatatgc ggttgcagat cgtgaaccaa tcactattca
     1261 taccgaagat gctgctcgct ttggtattgc gaatggtgat ctggtgcgtg tgtggaacaa
     1321 acgtggtcag attctgacag gcgcggtggt gactgacggg atcaaaaaag gcgtggtatg
     1381 cgtgcatgaa ggtgcatggc cagatctgga aaatggcttg tgtaaaaacg gcagtgcgaa
     1441 cgtgttaacg gcggatatcc ccagctcgca gctggcaaat gcctgtgccg gtaactctgc
     1501 gctggtgtat atcgaaaaat atacgggtaa cgcaccgaag ttaacggcat ttgataagcc
     1561 tgctgttcag gcgtaagaag tgaatagcgc ccggaaacgg gcgctgaggt tatgcaaacg
     1621 taaaactgcc tgatgcgcta tgcttatcag gcctacatag tccctgcaat agattgaatt
     1681 agcaagggtt tgtaggccgg ataaggcatt cacgctgcat ccggcagtaa taccagacct
     1741 ttacggcttg cgcgcaatca gcaccgcgcg tttaggcgca ggataacctt caactgtctt
     1801 actcggatca tgtgggtcga ggaaatcgga cagagattcg gtaaccatcc attcagtgcg
     1861 tcgctgctct tcagtagtgg taacgcacac atccacaacg cggatatcta caaaaccaca
     1921 cttcttcagc cagtttttca gcgccagcgc ggaagggatg aaatagacat tacgcatttg
     1981 cgcgtaacga tcgcctggta ccagcaccgt gttctcatcg ccatcaataa ccagcgtttc
     2041 cagcaccagt tcaccctcat tcaccagttg atctttcaac tgccagagat gctccagcgg
     2101 cgaacggcga tggtaaagca cgcccatcga aaagacggta tcaaaggctt tcagtgccgg
     2161 aagctgttca atacctaacg gtaacaaatg tgcgcgctga tcgttaccca gtagtttacg
     2221 caccgcttca aactggcaca gaaacagttg cgtagggtcg atccccaccg cgaggtgcgc
     2281 gcctgcgcca atcatgcgcc acatgtgata accgctgccg cagccgacat ccagaatggt
     2341 gcgtccggtt aaatcagaaa gatggggcag aacacgatcc catttccagt cggaacgcca
     2401 ttcggtatcg atgttgacgc catacagtga gaacggccct ttgcgccatg gcatcaggtt
     2461 ccgcataagc gtttcaatgc gtttaatttg cccggtgctc agtggctctt cgctttcggc
     2521 ggttacgcta tgcaataaat ccagacgata cggtttaatt tcaggcagaa attccaccgc
     2581 gttggaccac tgcttaaaca gcccgtgctg ctgctcgcgc tgccagttag caatctgcgc
     2641 gggcagcgtt tcgagccagt gtgaaagatg atttttggca atcagagaat aaaagttacc
     2701 aaagtcgatc atgcagcgtc ctctgctttt aatgccacca gtgaaccaaa gttaaagcac
     2761 tggaaccaca gctcgctatg ctcaaaaccg gctttatgca ggcgtgcttt atgggtttcc
     2821 acggaatcgg tcagcatcac gttttccagc atgctgcgct tctggctgat ctccagttcg
     2881 ctgtaaccgt tggcacgttt aaagtcgtgg tgcatgttga acaacagttc accaactttg
     2941 gcatcttcga aactgaattt ttccgaaagc accagcgcgc cgcccgggtt cagtccttga
     3001 taaattttat ccagtaatgc ctggcgctcg gaaggttcca ggaattgcag ggtaaaattc
     3061 agcaccacca tcgatgcgtt ttcaatggcg atatcgcgaa tatcaccttc aatgacgtct
     3121 actggcgtag gggctttata ggcgtcaata tgacgacggc agcgttcaat catcgccggg
     3181 gagttgtcga tggcaataat tttgcaatta tcatgatgaa tgttgcgacg caccgagagc
     3241 gtcgccgcgc ccagagaaca acccagatcg taaacctgcg taccaggttg aacgaagcgc
     3301 tcggctaaca tactaatcat ggaaataata ttggaatagc cgggaacgga acgctggatc
     3361 atatccggga agacttcagc tacccgttca tcaaaggtcc agtcgcccag tctggcgata
     3421 ggggcagaaa atagcgtgtc gcggtgagac ataacgtaaa aatccgggaa aaagaaagtg
     3481 gcgtattgtg cgctaacgca gggagaaaac caactcccag ggcatatacc aaagattcgc
     3541 cagcaccatc agcaacagcg cacaccaggt ggcgctcatg ccggaacgtc gccagcgaaa
     3601 cagacggtga tgaaaaccgt aataatgcat cagacgacca gcaagcaaaa cgatgccgca
     3661 aatatgcacc atccaggttt ctgcgccatt catttccata aacagcatca gcacaatcgc
     3721 gatgggaata tattccaccg cgttaccatg aatgcgaata gcgctttgca gttcgctaaa
     3781 accaccgtca ccataggcaa cgcggtactg caaacgcaa
//
LOCUS       DAJTSL010000248         3758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTSL010000248 DAJTSL010000000
VERSION     DAJTSL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.251"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1411)
                     /gene="maeB"
                     /locus_tag="OO433_003979"
     CDS             complement(<1..1411)
                     /gene="maeB"
                     /locus_tag="OO433_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6052263.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKP"
     gene            1700..2650
                     /gene="tal"
                     /locus_tag="OO433_003980"
     CDS             1700..2650
                     /gene="tal"
                     /locus_tag="OO433_003980"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6052264.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGISAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            2670..>3758
                     /locus_tag="OO433_003981"
     CDS             2670..>3758
                     /locus_tag="OO433_003981"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6052265.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTGYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNT"
BASE COUNT          926 a          980 c          971 g          881 t
ORIGIN      
        1 tcggtttcgc cagtcccagc gttaccagtt cctgagtggc atgcagaacg cgcgcctctt
       61 ccccttctgg cagaacaacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca
      121 taaacaggtt ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag
      181 caatcggacg cgtcgccacg cccgactcca tcgcggcttt agcgaccgca ggagcgatct
      241 taacgatcaa gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat
      301 cctgatcgcc atacgctgaa gccaccactt cgctctgttc cgcatgggcg agttctgcaa
      361 tcgcacgtac cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg
      421 cgccacggaa gatgaacggg aagcacagga cgttgttcac ctggttcgga tagtcagaac
      481 gaccggtgca aatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg
      541 gttccgggtt cgccagcgcc aggatcattg gcgcacgagc cattttcttc accatttcct
      601 gggtcagcac tttcgggccg gaacatccga ggaaaatatc cgcgccttca atcacatcat
      661 cgagggtacg tttgccgtcg tccaccaccg catatgcggc tttggtttcc gccatgtttg
      721 gctcacggcc ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca
      781 gacccagcgc taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca
      841 ccatccgcac gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag
      901 tgctgatgat tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc
      961 gcagtttctg ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgattccgc
     1021 cgaaggtcgg ttcgagcgca gcgacaactt caataaattt gtccgggtcg agttcatcaa
     1081 cttcaatgtc aaatacatca atcccggcga atttcttaaa cagaacgccc ttgccttcca
     1141 tcaccggttt gccagccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag
     1201 agatcaccgc caccaggtta ccgcgcgcgg tatatttgta ggcttttaac gggtcttttt
     1261 cgatttcaag acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg
     1321 ttgccagagg cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat
     1381 caagtgcact ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca
     1441 aataaagtgt gtgggcaaca gcccctgccc acaacgtggc gcacattatt accccgccgg
     1501 agtctacaga ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat
     1561 cctataacac tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt
     1621 ttgatgcgtt tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac
     1681 ttttcaagga gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt
     1741 ggcagacagc ggcgatattg agtccattcg ccattatcat ccccaggatg ccaccaccaa
     1801 tccttcgctg ttgctcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc
     1861 tatcgcctgg ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga
     1921 caaactggcg gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtc gcgtgtcaac
     1981 agaagttgat gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct
     2041 ggtggacttg tatcagcaac aaggcgttga gaaatcacgc attctgatca aactggcttc
     2101 gacctgggaa ggaattagcg cggcagaaga gctggaaaaa gaaggtatta actgcaacct
     2161 gacgctgctg ttttcttttg cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat
     2221 ttcgccgttt gtcgggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta
     2281 tgtggtggaa gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca
     2341 acaccactat gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc
     2401 cttaaccggc tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa
     2461 agtttcgcca gtggtacgta aattaatccc accttctcag acgttcccac gcccagctcc
     2521 catgagcgaa gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact
     2581 gtctgaaggc attcgtctgt tcgccgttga tcaacgcaaa ctggaagatc ttcttgccgc
     2641 caaactataa accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc
     2701 gcgcactcag tatggatgcg gtacaaaaag ccaactctgg tcatcccggc gcgccgatgg
     2761 gcatggctga tattgccgaa gtgctgtgga acgattttct taaacataac cctaccgacc
     2821 caaccgggta tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct
     2881 acagtttgct gcatctgacc ggttacgacc tgccgctgga agagctgaag aacttccgtc
     2941 agttgcattc gaaaacccca ggccacccgg agattggtta tacgcccggt gttgaaacca
     3001 ccaccggtcc acttggacaa ggtttggcga acgccgtcgg actggcgata gcagagcgta
     3061 cactggcggc gcagtttaat cagccggatc atgaaattgt cgatcacttc acctatgtgt
     3121 ttatgggcga cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcgggca
     3181 cgctaggact gggcaagctg attggttttt acgatcacaa cggtatttcg attgatggag
     3241 aaaccgaagg ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg
     3301 tgatccatga aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc
     3361 aaagcgtgaa agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc
     3421 cgaataaagc aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc
     3481 tggcacggca aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc
     3541 acgcctggga tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg
     3601 ccgcctataa aaaggctcat ccgcaactgg cagaagagtt tacccgacgg atgagcggtg
     3661 gtttaccgaa ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc
     3721 cggcgaaaat cgctacccgt aaggcttcgc aaaatacg
//
LOCUS       DAJTSL010000249         3755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTSL010000249 DAJTSL010000000
VERSION     DAJTSL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.252"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..343
                     /locus_tag="OO433_003982"
     CDS             <1..343
                     /locus_tag="OO433_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6052266.1"
                     /translation="LNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTG
                     DIFGTAGKIARIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFS
                     AWDKSGKLVKE"
     gene            351..1679
                     /locus_tag="OO433_003983"
     CDS             351..1679
                     /locus_tag="OO433_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6052267.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGSYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1726..3051)
                     /gene="rimO"
                     /locus_tag="OO433_003984"
     CDS             complement(1726..3051)
                     /gene="rimO"
                     /locus_tag="OO433_003984"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6052268.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            3264..3647
                     /gene="bssR"
                     /locus_tag="OO433_003985"
     CDS             3264..3647
                     /gene="bssR"
                     /locus_tag="OO433_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6052269.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSILSLQKHAMLEKA"
BASE COUNT          973 a          839 c          931 g         1012 t
ORIGIN      
        1 tctcaatatt gaacaactgc gtaaacaagg ggtacggata tcgattgatg actttggcac
       61 cggtttgtca aacctgaaac gtttttatga aattaatcca gacagcataa aggtggactc
      121 gcaatttacc ggcgatattt tcggtactgc gggaaaaatt gcgcgcatta ttttcgatct
      181 ggcacgctat aaccggatcc cggtgattgc ggaaggcgta gagagcgaag acgttgcacg
      241 cgaattaatt aaattaggat gtgttcaggc tcaggggtat ctgtaccaga aacctatgcc
      301 attctccgcc tgggataaaa gtggaaaatt agtaaaagag tagtttacgt atgtccagaa
      361 tcaataagtt cgtacttaca gtcagtctgc tgatttttat catgatttca gcagttgcct
      421 gcgggatcta cactcaaatg gtaaaggaac gggtgtatag cctgaaacag tccgttattg
      481 atactgcttt tgcggtggca aatattgctg aatatcggcg tagcgtggca attgatctta
      541 tcaacacgct aaatcccacg gaggaacagc tgttggttgg tttgcgcaca gcttacgccg
      601 actcggtttc cccctcttat ttgtacgatg tcggtcctta tctgatttcc agtgacgaat
      661 gtattcaggt aaaggagttc gagaaaaatt attgtgcaga tattatgcag gttgtgaagt
      721 atcgacatgt caaaaataca gggtttatct cttttgacgg taaaaccttc gtctattacc
      781 tctatccggt aactcacaat cgtagtctga tatttttgct tggtctggag cgtttttctt
      841 tactgtcaaa atcgctggcg atggacagcg agaacctgat gttctctcta tttaagaacg
      901 gtaaaccggt gaccggtgat gaatataatg ctaaaaacgc catcttcacc gtttcggaag
      961 cgatggagca cttcgcctat ttgccgaccg gattgtacgt atttgcgtat aaaaaagatg
     1021 tttatttgcg ggtctgtaca ttgattattt tctttgccgc attggtggct gtgatatcgg
     1081 gtgccagttg cctctatctg gtacgcagag tgattaatcg tggtattgtg gagaaagaag
     1141 ccatcattaa taaccatttt gaacgcgtac tggatggcgg gcttttcttt tcggctgccg
     1201 atgtcaaaaa actctacagt atgtataact cggcgttcct ggacgacctg accaaagcaa
     1261 tgggcagaaa atcctttgac gaagatttaa aagcgctgcc ggaaaaaggc agttatttgt
     1321 gcctgtttga cgtcgataaa ttcaaaaaca ttaacgacac cttcggtcat ttgctgggcg
     1381 atgaagtgtt gatgaaagtg gtgaaaatcc ttaaatcaca gatcccggta gataaaggta
     1441 aaatctaccg cttcggcggt gacgaatttg cggtgattta tacgggtgga acgctggaag
     1501 agttgctatc gattctaaaa gaaatcgttc atttccaggt gggaagcatt aatttaagta
     1561 ccagtatcgg tgtagcgcat tcaaatgaat gtaccaccgt cgaacgcttg aaaatgctgg
     1621 cggatgagcg gctgtataag agtaagaaaa acggcagggc tcagattagc tggcagtaat
     1681 cattattcgc aggccggaca aatgattttg cccggcctga attaattaaa cccggctacc
     1741 ccacaaatcg tactcatcgg cgtgctcgac tttcacacgc aggatatcac ccggcttaac
     1801 gttggtttca ccattgagat aaaccgcgcc gtcgatttcc ggtgcatctg ccatgctgcg
     1861 accaatcgcg ccttcttcgt ccacttcgtc gataatcacc agaatttcac ggcccacttt
     1921 ctcttgcagg cgctcggcgg aaatctgctg ctgcagctgc atgaaacggt tccagcgttc
     1981 ttctttaact tcttccggaa cctggtcagg cagggcattg gcgtctgcac cttcaaccgg
     2041 gctgtattta aagcagccaa cgcgatccag acgcgcttct ttcaggaagt cgagtagcat
     2101 ctggaaatct tcttctgtct cgccagggaa gccgacaata aaggttgagc gtagggtcag
     2161 ttccgggcag atttcgcgcc actgtttgat gcgcgccagt tggcgatcta cagaacccgg
     2221 acgcttcatc agtttgagaa tgcgcgggct ggcgtgctgc aacggaatgt ccagatacgg
     2281 caggattttg ccttctgcca tcagtgggat gacgtcgtcc acatgcggat aagggtaaac
     2341 gtagtgcaga cgtgtccaga tccccagttt cgataactgt tcgcacaggc tgaccatgct
     2401 ggtttttacc ggctcgccgt tgtggaagcc agtacgatgt ttaacatcaa cgccataggc
     2461 ggaagtatcc tgcgagatca ccagaatctc tttaacgccc gcatctacca gacgtttcgc
     2521 ttcacttaac acttcgccaa tcggacggct caccaggtcg ccgcgcatag acggaataat
     2581 gcagaaggtg cagcggtgat tacagccttc cgaaattttc agataggcat aatgacgcgg
     2641 cgtcagtttc acaccttgtt ctggcaccag gctcaggaat gggttgtgtt tcggttttgg
     2701 cacgtagtga tgaacgtgct ccagaacctg ctcatagcta tgaggcccgg tgatttccag
     2761 cactttcggg tggacttcgc ggatctgatc ttcttttgcc cccagacaac cggtcacaat
     2821 taccttgccg ttttcattca acgcttcacc aatggcttcc agtgattctt gtaccgcgct
     2881 gtcaataaag ccgcaggtgt tgacgatcac catgtccgca tcgtcatagc tcggtaccac
     2941 gtcataacct tcagtgcgga gttcggtgag aatacgctct gaatcgacaa ggtttttcgg
     3001 acagccaagg gaaacaaagc cgattttcgg ctggggagtt actttgctca tagcttaaaa
     3061 aatattcagt tacaggaaag gtcagggcag ggattctaca gagttctgga taaaatttgt
     3121 atcgcaatct cattcgctgg cggaggcgaa ggaaatgtaa attttgttaa ttcggcgtga
     3181 agaattgatc ctggacagca ttttgctcaa aaaatagcca tactatttaa ttgcaacaag
     3241 gctggaaaga ggaggatcga agtatgttcg ttgacagaca gcgaatcgat ctgctgaacc
     3301 ggttgatcga cgcacgcgtt gacctcgctg catacgtgca actgaggaag gcaaaaggat
     3361 acatgtccgt cagcgaaagc aatcatctac gagataactt ttttaaactg aatcgcgaac
     3421 tgcacgataa atcgctgcgg ttgaatcttc atctggatca ggaagagtgg agtgctcttc
     3481 atcatgctga agaagcatta gcgacagccg cagtatgttt gatgagtggg caccatgatt
     3541 gcccgactgt tattaccgtc aacgccgata agcttgaaaa ttgtctgatg agcttaacgc
     3601 tgagtatcct gagcctgcag aagcacgcca tgcttgagaa ggcctgaaaa ctaaggggga
     3661 gaaagcgtct cccccttcat gtttaagttt ttgtaaaaat gaatttgtta tctcctccac
     3721 tgactacgct ttaagccaga gtcaatccgg aggcg
//
LOCUS       DAJTSL010000250         3739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTSL010000250 DAJTSL010000000
VERSION     DAJTSL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.253"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..861)
                     /locus_tag="OO433_003986"
     CDS             complement(<1..861)
                     /locus_tag="OO433_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6052270.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEI"
     gene            complement(886..1764)
                     /gene="yihU"
                     /locus_tag="OO433_003987"
     CDS             complement(886..1764)
                     /gene="yihU"
                     /locus_tag="OO433_003987"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6052271.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTQAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGTPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1937..2833
                     /locus_tag="OO433_003988"
     CDS             1937..2833
                     /locus_tag="OO433_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6052272.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYNLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            2873..3661
                     /locus_tag="OO433_003989"
     CDS             2873..3661
                     /locus_tag="OO433_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6052273.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDLNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
BASE COUNT          963 a          955 c          903 g          918 t
ORIGIN      
        1 aatttctgcc aaccgttgta aacgaggaac agagacatcg cgcagcatag tttgtggatc
       61 ttgtgcacca acaaccgacg cccaaaccgc tcgtccagcc aggaagcccg acgcaccacc
      121 tttcatggca atacttactg cacggccaaa aatatcggca tcaacgccag aagaaagaat
      181 tacccacggc atcttcatct gatcgttaag ttgctgacag gcggcaagta aggttttttc
      241 atcgcctttg ccccctaatg gcatctcggc tttgtaaaga tcagcctctg ttccaccaag
      301 ctctgcggcg gcggcaacaa tggcgctttc acgatcaaag tcccaaccgc gacgaggtgg
      361 acgcaccaca ggttcaataa tgctcaccag cccggcacta cggcagcgac gaacaaattt
      421 atcaaccatt gccagacgtt cctctgcggg ttcatcttca cgccagagta caagtaattt
      481 cattgcctta gcccccattt cacgggcttt atgcgggtcg acggacatat ctatttcaac
      541 gctatcaacc gggatgccat tacctgggat aaaaagatcg gcagcgacaa tcaggccgca
      601 agaattagca acagcgccct gctcgactat ttgctcaaga caaaattgtt tatccgccag
      661 aacggcagaa gcataaggag aaaggattcg ggtcgcggca actttaaaat cggtgagtac
      721 actgtcggcg atgggttttg gctgccctgc tgccgcaaac atcaagcgca tggcctcacg
      781 ctgatcgaca gctaacatgg caaatccacc acctgggcgg gagatatctt tcagcgtaaa
      841 tggggtgaag tgggatgaca tagaaactcc tgttaataga taaaattaaa gaccagcctg
      901 ctggcgaacc tgatttagca gggcactcca gtcctgccgc cctttgccgt cttgacttgc
      961 tgcagcgtaa taactttctg ctgcaatccc catattgagc ggtgtccccg tctcccgagc
     1021 gacgtcaact gcaatacgca gatctttcaa cgccagatcc accatgaagg cgggtgaaag
     1081 atcgcctttc agcactttgc caggccaggt ggtcgtaaaa tgtcctttcc ctgctgccgt
     1141 accactcatc accttaattg cgacatcaag atttaatccc agtgactcac ataacacagc
     1201 ggcctcggag gacagcgcat taagcgcaat gctcatatag ttgttaatta gcttcacgcg
     1261 gatccccatt cctgggccgc cagcctcgac cagctcatta cccatgcaca taagaatcgg
     1321 gcgtgcacgt gcaatctgct catcagtgcc acccgctaaa atcagcaatg tgccatcaat
     1381 cgcattcacg gacgttctgc ccacgggagc gtccatcata ctgatgcctt tttcttgtaa
     1441 ctcccgtatt aatgcatcgg tctgtaaggg atgaatagtc gacatatcaa tcaataacgc
     1501 gtcagatgaa acagcctcac agacgccttt ttcccccaac agaacctgtc gaacaatgtc
     1561 accattggga agcatagtga tgataaattc agcgcctgct gctgcttccg cttgggtttg
     1621 tgccgcagtg gccccctgcg taactagcgc atcgacggcc tgtgcattga catcaaatac
     1681 ctgcaatgaa tgcccttttt gcaatagatt gttagccatt gggctaccca tctgccccag
     1741 cccgagaaat gcaatggttg ccatgaatac tctcctgtct gacattgaca aaataatgac
     1801 aattgaaaat tcgtttttgt ctgtttttga gcatatttgt gatttatgtt caaaaacacg
     1861 acacgcatca ctaatttgat catttgcgtc atttaaattg accaaagacg acaaaattaa
     1921 acaggacaag acaaatatga ccagaattgc atgcgtgggt atcacagttc aggacagaat
     1981 ttattctttg cccactctgc cggagggcgg cgggaaatat caggcaaatc attatcttga
     2041 gattggtggc ggcccggcag caacagccgc tgttgcaatc gcaaagcttg gcgtggaggt
     2101 tgatttcatc ggtcgcgtcg gcgatgacag ttgtggaaat accttacttg cagaacttga
     2161 gggttggggc gtcaataccg cattttgccg ccgatatcct aacgcccgtt cgtcgcaatc
     2221 agcgatcctc gttgatcagc atggcgaacg gattatcgtt aattatccaa gtcctgatct
     2281 cggtactgat gccgaatggt tagaagcgat cgatttctct cgttataatc tgattctggc
     2341 cgatgttcgc tggcatagcg gtactgaaaa agcattctcg ctagcgcgcc ttgcgggtgt
     2401 gacaacgctt ttagacgccg atatgacacc gcaggatatt tctccacttg tcgcgcttgc
     2461 cgatcacgcc gtgttctcaa caccagggct gaaacgcatg actggcctgc agtctcctga
     2521 agaaggtttg ttccaggcaa cgacacaaac agcaggtaaa gtgtatgtta cgctgggttc
     2581 tgaaggatcg ttgtggattg aagatggtca tttgtgtcaa caggaggcat tttctgtcaa
     2641 tgtggttgat accactggag caggcgatgt ttttcacggc gcactcgcgg tagcgttggc
     2701 agaaaaaatg ccaacaaaag aggctattcg ttttgccagt gcggttgccg cgatgaaatg
     2761 tacccagcct ggcggtcggg ccggcattcc taatcgtgag caaaccgaat catttttgtc
     2821 actttatgcg taaaatggta aacatctacc gaggaggggc aagggagtat tcatgagtat
     2881 catcgaagtg acaggtaatc cacgccatga ccaactcgtt catctaatcg cagaacgcgg
     2941 ttatatgaat attgaagaac tggcacaact gctggatgtt tcaacgcaaa ccgtgcgccg
     3001 ggatatccga aaactcagtg agcagggctt gatcacccgt catcatggag gggccgggcg
     3061 cgtctccagc gtcatgaata ccgcttttga gcaacgggaa ctttcgctca ccgccgagaa
     3121 acgggcgatc gctgaggcag tcgccgatta ccttcccgaa cgctgtaccg tctttatcac
     3181 catcggtaca accgtagaag ccgttgccag ggcattactc aaccggcgtg atttacgcat
     3241 tatcactaac agcctacgtg tggcacagat tctttataag aatcaggata ttgaagtgat
     3301 ggtgccggga ggcactttac gcgctcataa cggcgggatt atcggcccag gagccgtgga
     3361 ttttattgaa ggcttccgcg cagattattt aatcaccagt ataggtgcca ttgaacatga
     3421 cggcacccta ctggaatttg atctaaatga agcgttagtc gcgagaacga tgattaaaca
     3481 tgcgcggaat acattgttag tggccgatca tacgaagttt gccgcgtctg ctgccgtttc
     3541 aattggcaat gcacggaatg tcagggcttt ttttactgat gccccgcctc ccaattcttt
     3601 ctgccagttg ttaagtgaag agaatgttga actggtggtt gccgagcaag aagtatcctg
     3661 aaattgaaat ttcggaaaat ccgctgggaa tttcagcgga ttttgttttc cataagcaaa
     3721 aaaaacctgc cagcgatgg
//
LOCUS       DAJTSL010000251         3729 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTSL010000251 DAJTSL010000000
VERSION     DAJTSL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.254"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..329
                     /locus_tag="OO433_003990"
                     /pseudo
     CDS             1..329
                     /locus_tag="OO433_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            446..595
                     /locus_tag="OO433_003991"
     CDS             446..595
                     /locus_tag="OO433_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001500869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6052274.1"
                     /translation="MSHYKVQQMLSCQKRRQKVQPVMQSGMQRPQQKKPGSQQKAHSQ
                     RNKAG"
     gene            628..1291
                     /locus_tag="OO433_003992"
                     /pseudo
     CDS             628..1291
                     /locus_tag="OO433_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117976.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            1377..1889
                     /locus_tag="OO433_003993"
     CDS             1377..1889
                     /locus_tag="OO433_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052275.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLSKPFMMSQMVGRTM
                     GCKIATEYYKWKSSDKVDNWGAVGADWPLEEKSKGTITNAASCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVPQDLKIS"
     gene            1965..2177
                     /locus_tag="OO433_003994"
     CDS             1965..2177
                     /locus_tag="OO433_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6052276.1"
                     /translation="MPEKFFRTDADNNDVPMTAASWMALSEATEQAMFAKGVEINTRQ
                     LQMKAEVEALTDLKAIRSYVVGWPAG"
     gene            complement(2319..2644)
                     /locus_tag="OO433_003995"
                     /pseudo
     CDS             complement(2319..2644)
                     /locus_tag="OO433_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(2780..3028)
                     /locus_tag="OO433_003996"
     CDS             complement(2780..3028)
                     /locus_tag="OO433_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6052277.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(3383..>3729)
                     /locus_tag="OO433_003997"
     CDS             complement(3383..>3729)
                     /locus_tag="OO433_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6052278.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT         1006 a          796 c         1037 g          890 t
ORIGIN      
        1 tcagaaaatc cggatgaagc cgggcgttac agtatggacg ttgagtatgg tcagtacagc
       61 gttattctgt tggtggaggg attcccgccg tcacatgccg ggactatcac cgtgtatgaa
      121 gattctcaac ccggtacgct gaatgatttt ctcggtgcca tgactgagga tgatgcccgt
      181 ccggaggcac tgcgtcgctt tgaactgatg gtggaagagg tggtgcgtaa cgcagaggag
      241 gcgaagaaga atgccggaga ggcggagacg tcagcgagga atgccggcat atcagccagt
      301 caggcagaag agagcgctgc aaatgctgac acttcagcag gggatgcatc ggagtcagcc
      361 cggcaggcgg cagaaagtgc agcctcagca aagcagtcag aggaggcgtc ctcgtcctcg
      421 gcctctgcgg ccgctcaaaa agccagtgag tcattacaaa gtgcaacaga tgctgagttg
      481 tcaaaaaaga cggcagaaag tgcagccggt aatgcagtca gggatgcaac gaccgcaaca
      541 gaaaaagccc gggagtcagc agaaagcgca cagtcagcgg aacaaagcag gatagcggcg
      601 gaagaggccg taaaccgaat ccccaccgtg gtgggacctc ccgggccaaa gggggaaccg
      661 gggcccgcgg gtcctcaggg gccgaaggga gataaaggag agcgtggcga caccggcccg
      721 gcaggggcca aaaggtgaca aaggcgatcc gggagtggct ggaccagcag gtccggcagg
      781 tgcgccgggg ccgaaaggcg ataaaggtga tccgggagta gcaggtccag caggtccggc
      841 ggggccacag ggaccgaagg gagacacagg agcccccggg caaggaacag aactgcttac
      901 tactgccaat acatggactc aggcacaaac ttttaatggt ggtattaatg gcaatttgac
      961 ggtgaccgga aacggctcat ttaacgatat tcagatccgt tcggataaac gcaacaagcg
     1021 aaatctggta aaactggata atgcgttaga tcgtctggag gcacttactg gttatcttta
     1081 cgagatacag tactctgccg acggttggca aacgtcggtt ggtttaattg ctcaggatgc
     1141 acaaaaagca ttgcctgaac tggtaactga agacgcagac gttatatctg gtgaaaaacg
     1201 tctgcgtctt aactacaacg gcataattgc attgttagtc gagggcttta aaacacttcg
     1261 tcatgagatt aaagaactcc gggagaagta aacgacagct gttgtagttt ctggtttcta
     1321 ctgaatttaa attgtggggt gacactcacc ccacgcattc agaaggggga gatgagatgg
     1381 gggtaacatc gggatgggta ggctcttcgg ctaagagcga aacaggtgag cagtggatgg
     1441 gagccgccgg aaccaaactg ggactgagta aaccttttat gatgagccaa atggttgggc
     1501 gaactatggg ctgtaaaatt gcaactgagt actataaatg gaaatcctct gacaaggttg
     1561 ataactgggg cgcagttggc gctgactggc ctttagaaga aaaaagtaaa ggtacaatta
     1621 caaacgccgc aagctgcgga tcagggaggc tggtaggggc tgtcgttaca ctttctcact
     1681 ttttgacgaa ctctacaccg acagctgctg tttatttagc cggtggtaaa gcaggtaaca
     1741 tcaccgtaaa cgtaggtggt gctacacaaa ccatgattta tcagggcgtt gttagtgggt
     1801 tccagtatta ctggtcaggt tctgttagtt ccgctttcgt ggaggcaatg aaaaagacgg
     1861 gagtacccca ggatctaaaa attagttaaa tggtaaataa ataattttaa aaacttcacg
     1921 ttctacatac cggagacgcc ggatatatag gatattgttt taagttgcca gagaaatttt
     1981 tccggacgga tgctgataat aatgatgtgc caatgacggc agcatcatgg atggcactga
     2041 gtgaagcgac agagcaggcg atgtttgcga agggagtgga gattaataca cggcagttgc
     2101 agatgaaagc cgaggttgag gcgctgacag acctgaaggc gatccgcagt tatgttgtcg
     2161 gatggcctgc tggctgaata aaaaaacggg accacgacca gtcccggaac catgagtttt
     2221 agggtattag tttgttatca tagttagcgt gctaagtatg ccatgtcagg ttgattagtg
     2281 aagtgatgtt gttcgcattt ttgcacggcg gagaatattc agatttttgg aaatccatat
     2341 ttttcccgtg cgcgattaca tgcttcattt acgatgctat tttcgccgga cattgcgaat
     2401 tccctgcatg tggacggttt ttgtaaacag agcaatatgc gttttctccg gggggggccg
     2461 gcaagagcgc cacatcgggg atttttctga ttggtgccgc gcatacagcg atgaaaagga
     2521 gatatttgct cagtgagact gaccgaaacc tttccgccag catcgtcagc ttctgcccag
     2581 taaaaagaga cgcggaaaat gcacaacagg caccacacgt catgcatgga ttcagattgc
     2641 tcataattca ccagtacagc tataaatcgt aaagagaaca gcggtacatc gtatgtaaga
     2701 acgtaccgcg gctggctggt gaacttccga tagtgcgagt attgaatgat ttccagccgt
     2761 taccgatttt acgtgctaat tagtgaacaa accactcgtc agcagactcc caggtatctt
     2821 tcagagtttc ctgaacaaaa gttttagctg aatctttatc ggcggtgcgc gtaacagaaa
     2881 ggccatcgtt gctggtagct tttacgatca cctctacatc gtcataacgt ttactgatgc
     2941 gtcgggttaa ttcttccttt aacgcatcca cagcaccggt tggcatttta gtcatttttt
     3001 ctttggctat gcagatttca atacgcataa aagtccctct acactgtgtt tgtatacagt
     3061 atcattttta actgtatgga taaacagtat taagggtgaa ggaaagtgat tttttggggc
     3121 atgcatgggg caaaaatatt tgttgtgggg cattttgggg catgattggg acactttgtt
     3181 acatatgaac tttgccgatt tttatatgac atggaaatac aacgctttga ttcttctaaa
     3241 taaaaaacat gctcttgggc gttctttagt gatttttaaa aattccgtat cacgcagtta
     3301 aagtggcggg catactcttc aaggctggtg atccccaacc gcacccattt aggatgcgac
     3361 cattgcggta gaccgatgta aatcataacg cgtgaaggat ctcttccacg ctacgcacac
     3421 gggcgatgcg cgggtagata tgattaatgc tgttattgtg ttgctcggcg ctggcggcac
     3481 tacaggcgtc ttcggcaatc accagattaa agccaagttc ccaggcattg cgggcggtgg
     3541 attcaacacc gatattggtc gagatcccac ataacactat tgtatcgata cctcggcggc
     3601 gcagttgcaa ctccagatcc gtaccgtaaa acgcacccca ctgacgtttg atgatttcga
     3661 tatcactgtc agttgcacct aatgcagctg gatgttgcca ccagttttcg ggcaacactt
     3721 tagcggggg
//
LOCUS       DAJTSL010000252         3724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTSL010000252 DAJTSL010000000
VERSION     DAJTSL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.255"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            37..264
                     /locus_tag="OO433_003998"
     CDS             37..264
                     /locus_tag="OO433_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6052279.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(265..639)
                     /locus_tag="OO433_003999"
     CDS             complement(265..639)
                     /locus_tag="OO433_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6052280.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="OO433_004000"
                     /pseudo
     CDS             complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="OO433_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2118..3038)
                     /gene="lpxL"
                     /locus_tag="OO433_004001"
     CDS             complement(2118..3038)
                     /gene="lpxL"
                     /locus_tag="OO433_004001"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6052281.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3263..3724
                     /locus_tag="OO433_004002"
                     /pseudo
     CDS             3263..3724
                     /locus_tag="OO433_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
BASE COUNT          915 a          952 c          945 g          912 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc attattagtc gcactatacc ggggcagggg
      121 catggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ctggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 attcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg ctgcgcgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcgtatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg cccggcgatc tggttgctcg agttgtaaac caacaatgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgct gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga ccggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggtggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ccaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctga agacaatacc ggaccatatt cagggcggag
     1801 tgaccggtaa cgccaagctg ctcaacgtag aggggtaaga agggcattac cagactgaag
     1861 gcggcaccgg taagaaaaca gcctagccag gcgacgatca ggtttcgttt ccagtttata
     1921 ggggtgtcat tttcacaggg tgacatagca atccgctgtt ggtgcgccag gcgcggtgaa
     1981 cataagaaga aaagataagc acactaatta tgcgcccgac ttccaggggg cgcaatccag
     2041 agagctttta tcgctaaatc agggggattt gctgtggtaa tgccggatgc cattctgaag
     2101 catccggcag gggagattta atagcgtgaa ggaacgcctt ccgggcgtgt tttaaagcga
     2161 cggtgtaacc acatatactg ctctggtgcc atcatgatgc atttttcgac cactttgttc
     2221 atccacgcgg cggtagtttc ggcatcatcc agtggcggag aacactctgg cggcagcata
     2281 atcaattgat accctttgcc atctggctta cggcgtggaa cgaagggcac cagacatgcg
     2341 ccggacatcc gtgccagcat ccaggttccg gtcgtggtcg cagcctgctc aacggcaaat
     2401 aacgggacga aaacgcttga gcgcgggccg taatcatgat ccggtgcgta ccagaccact
     2461 tcgccttttt tcagggcttt aatcatgcct tttaaatctt tgcggtcgag catcgattta
     2521 tttgagcgca aacggcccca ggtttgtagc cagtcaatca gtggattatc gttcgggcgg
     2581 taaacgccaa tccccggttc ctgcatacca aactgccgcg cacccagctc cagtgtcaga
     2641 aaatggatgc caactaacag gatgccgcgt ttttgcgcct gcacgtcacg aatgtgttcc
     2701 atgccgatca cttccgtcca gcgcgcgatt cggcggtccg gccagaacca cgccatgccg
     2761 gtttccatca ggcccatgcc aacggattcg aaattcttca ccaccatttt acggcgttct
     2821 tgttcgctca tttccgggaa gcacagttcc aggttgcgat gcacaatttt tgcgcgtcgt
     2881 ttcataaaac gtaacgccag ttttcctaat ccacaaccga ggcggtagat aaccgggtag
     2941 ggcaattgca cgactaacca aagtacgcca atacccaacc aggttaacca ataacgcgga
     3001 tgaagcagtg cggtggagaa cttgggtaga ttcgtcatat caatcctgtt tttcaaccta
     3061 ttcgggcaat tgtatgtatt gtcgcatttt ttcgcccgca accaaaattt gtggctgaag
     3121 actgggcgaa attgccgcgc ttgtaaataa caaataattt ttaatgcgca aatgtagcgt
     3181 aaaatgtgtg gatgttaatt atcgataatt gctatatcat gccgcggatt tttactttcc
     3241 catctcgcag gaaccgtaca ccatgccagt gttacacaac cgcatttcca acgacgcgct
     3301 aaaagccaaa atgttggctg agagcgaacc gcgaaccacc atttcgtttt acaagtattt
     3361 ccacatcgcc gatcctaagg cgacccgtga cgctttatat cagctgttta ccgcgctgaa
     3421 tgtttttggg cgagtgtatc tggcgcatga gggcattaac gcgcaaatca gcgtacctgc
     3481 gagcaatgtt gaaacatttc gcgcgcagct ctgccttcga cccggcttta gagggtttac
     3541 gcctgaatat cgcgttggat gatgacggga aatccttctg ggtactgcgc atgaaggtac
     3601 gtgatcgcat tgttgccgac ggtattgacg atcctcactt tgatgccagc aatgttggtg
     3661 agtatctgca agcggcggaa gtgaacgcca tgcttgacga tcccgatgca ctatttatcg
     3721 acat
//
LOCUS       DAJTSL010000253         3679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTSL010000253 DAJTSL010000000
VERSION     DAJTSL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.256"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(120..2189)
                     /gene="glyS"
                     /locus_tag="OO433_004003"
     CDS             complement(120..2189)
                     /gene="glyS"
                     /locus_tag="OO433_004003"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6052282.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLVESFAANFTVELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2199..3110)
                     /gene="glyQ"
                     /locus_tag="OO433_004004"
     CDS             complement(2199..3110)
                     /gene="glyQ"
                     /locus_tag="OO433_004004"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6052283.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3205..3504)
                     /locus_tag="OO433_004005"
     CDS             complement(3205..3504)
                     /locus_tag="OO433_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6052284.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
BASE COUNT          850 a         1001 c          947 g          881 t
ORIGIN      
        1 ctcataaaat taatatatga gataatcttt attcagcaga agattattaa aggttgctgt
       61 attatttagc gataataaaa aaagcctgcc agatggcagg ctttttaata acggcgttat
      121 tactgcaaca gcgaaatatc cgcaacgcgc aggaacagtt cgcgcagttt ctccagcatg
      181 gtcagacggt tgagacgcaa ttctttgtca tcaaccatga ccatcacttt atcgaagaaa
      241 gcatcaaccg gctcacgcag ctctgccagt tcgaccagcg catcctggta acgaccttcc
      301 gcaaagtacg gctccagctt gtcacgtagc accacaacct gcatcgccag tttaatttct
      361 tccggctctt tcagggtaga ggcattcacg cggtcgctca gcacttcgtc agatttcgcc
      421 agaatgttag atacacgttt gttcgccgcc gccagtgcag cagctgcatc cagggtacgg
      481 aaatgcgata ccgctttcat acgggcatcg aaatcagccg gacgagtcgg acgacgcgcc
      541 agtaccgcct ggatggtgtc aacggtgtaa ccttcgtcct gataccaggc gcggaagcga
      601 ccgagcataa agtcgataac atcatcaacc acgttggcgt tagtcagctt atcgccatac
      661 agacgcactg cttcttcggt cagcgtttgc agatcaaggt tgaggttctt ctcaacgata
      721 attcgcagca cgccaagtgc ggcacgacgc agcgcaaacg ggtctttgtc gcctttcgga
      781 tgctgaccga taccgaagat acccgccaga gtatccatct tatcagcaat cgccagcgca
      841 caagctaccg ggttggacgg caggtcatca ccggcaaagc gcggctgata ctgctcattc
      901 agcgcgacgg ctacgtcttc cgcttcgcca tcgtgacgcg cgtagtgcat ccccataacg
      961 ccttgggtgt cggtgaactc gaagaccatg ttggtcatca ggtcacactt agacagcagg
     1021 cccgcacggg ttgcgtggtt aacgtcagcg ccaatctgtt cagcaatcca gccagccagc
     1081 gcctggatgc ggtcagtttt gtcacgcagc gtacccagct gttgctggaa cagcacggtt
     1141 tgcaggcgcg gcaggttatc ttccagacgt tttttacggt cggtattgaa gaagaactcg
     1201 gcatccgcca gacgtggacg aacgactttc tcgttaccag agataatttg ctgcggatct
     1261 ttcgattcga tgttggcaac gaagatgaag ttcggcagca gtttgccatc gttcgcatac
     1321 accgggaagt atttctggtc acctttcatg gtgtaaacca gggcttcagc cggcaccgcg
     1381 aggaatttct cttcgaattt tgcggtcaga acgaccggcc actccaccag cgaagccact
     1441 tcttccagca ggctttcgct taagtcagcg ttaccgccaa tcttacgcgc tgcttcttcg
     1501 gcatcggctt taatcttcgc tttacgttct tcgtaatcgg cgatgacttt cccgcgctca
     1561 cgcagaattt ccggatactg atcggcgttg tcgatggtga attccggttc gcccataaag
     1621 cggtggccgc gaatcacgcg atcggactga atgcccagaa tagttgccgg aatgactttg
     1681 tcgcccagca gcagggtcac ggtgtgcacc ggacgcacga agtgcacgtc gcttgcgccc
     1741 caacgcatca gtttcgggat cggcagtttc gccagagaag tcgcaaccat attcggcagc
     1801 agtgcttcgg tgctttcgcc cttcacatgg gcgcgataca gcagccattc gcctttatcg
     1861 gtagtcagac gctcagcctg gtcaacggta ataccgcaac cacgcgccca accttctgcc
     1921 gctttgctcg gtttgccttc agcgtcgaac gcctgggcaa tcgccgggcc gcgtttttcg
     1981 atttcacgat ccggttgcgc ttccgccagg ttagctactt tcagcgccag acgacgcgga
     2041 gcagcaaacc attgaacggt gccgtgtgcg aggccagcgt tatccagctc cacagtaaaa
     2101 ttcgcagcaa aggactcaac caggctgcgc agtgcttttg gtggcagctc ttcagtgccg
     2161 atttccacca gaaaagtttt ctcagacata gccgcctctt acttatcttt gttgcacatc
     2221 gggaagccga gggcttcacg ggaagcgtag tatgcttctg ccactgcttt ggtcagggtg
     2281 cgaatgcgca gaatgtagcg ctgacgctcg gtgacggaga tggctttacg cgcatccagc
     2341 aggttgaagc tgtgggcggc tttcagaata cgctcgtagg ctggcagcgg cagcggattt
     2401 tccagcgcca gcagctgctg cgcttctttc tcgtactgct caaagcaggt gaacaggaag
     2461 tccacatccg cgtattcgaa gttgtaagtg gactgctcca cttcgttctg atggaacacg
     2521 tcgccgtagg tggttttacc cagcgggccg tcgctccaga ccaggtcgta aacgctgtct
     2581 acgccctgaa tgtacatggc cagacgttcc agaccgtagg tgatctcgcc ggtaaccggt
     2641 ttacactcca gaccaccaac ctgctggaag taagtgaact gcgtcacttc catgccgttt
     2701 agccacactt cccagcccag tccccaggca cccagagtcg ggttttccca gttatcttcc
     2761 acgaaacgga tgtcgtgaat agtcgggtcc atgcccagct ctttcagaga accgaggtac
     2821 agctcctgaa tattgtccgg cgatggctta atgaccacct ggaactgata gtagtgctgt
     2881 aaacggttgg ggttttcgcc gtagcgacca tcggtcgggc gacgagaagg ctgaacataa
     2941 gcagccgcca tcggttctgg ccccagcgcg cgcagacagg tcattgggtg agaggttccc
     3001 gcgccgactt ccatgtccaa tggttgaaca atggtgcagc cctggcgagc ccagtaatcc
     3061 tgtaaggtca ggatcaagcc ctggaaggtc ctggtatcaa acttttgcat attatttcgt
     3121 gctggatacg tgtggattta aaggaaggga tcagtatacc cgctggatgg aagatataca
     3181 gtacgaaacg ggaaaaagca gggcttaacg catggaaaga tgcaaaaaat ggccatctgc
     3241 gtcaaaagaa cagacaaact gctctttacg gaaggtataa ccgcgcattt catagcttcc
     3301 ctggaactgt tcgaaggcgg tgacgtcgat tttttgctcg ccggtgttat aacgttttgc
     3361 cgcctgatcc ttgcataacg cttccatatt caacgaacgc agaggatcaa cctttacccg
     3421 ttgtgctttc tgcacgggtg acggtgtaga acaacccact agcaccataa gcgccattgc
     3481 cggaaagaaa gcgttcatca tcattaaatt accgtctggt ttgcaggtga gtcttattat
     3541 ttatatcggt atagtggctg atatcttacg aatttcctta agcctctata agctgtagtg
     3601 ataaaactca gattattcca gtgcgccaac caaatgattt atcggtcaga ctttttttaa
     3661 ccagatacag agacacaga
//
LOCUS       DAJTSL010000254         3669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTSL010000254 DAJTSL010000000
VERSION     DAJTSL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.257"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(157..471)
                     /gene="fliE"
                     /locus_tag="OO433_004006"
     CDS             complement(157..471)
                     /gene="fliE"
                     /locus_tag="OO433_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6052285.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            686..2344
                     /gene="fliF"
                     /locus_tag="OO433_004007"
     CDS             686..2344
                     /gene="fliF"
                     /locus_tag="OO433_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR6052286.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     LSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTSRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2337..3332
                     /gene="fliG"
                     /locus_tag="OO433_004008"
     CDS             2337..3332
                     /gene="fliG"
                     /locus_tag="OO433_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6052287.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     VATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3325..>3669
                     /gene="fliH"
                     /locus_tag="OO433_004009"
     CDS             3325..>3669
                     /gene="fliH"
                     /locus_tag="OO433_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="binds to and inhibits the function of flagella
                     specific ATPase FliI; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6052288.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEADPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAKAKSQQAPIHARMQQLV
                     SEFQTTLDALDSV"
BASE COUNT          923 a          964 c         1028 g          754 t
ORIGIN      
        1 caaagcgaag acaaaacatg ttatcgcgtt tacggatcgt ggatggcaga aaactttcag
       61 gatcaggtat ccattttaaa ccagaaatag ggtgagtttg tcccatccat gccccacgcg
      121 ggcagatggg atgtaattac caacaggtta caaaacctac acctgcatgg acatcacttc
      181 ctgatacgcc gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc
      241 tttttgcata tcggtcatca catcgtttaa cgccacgccg ggttcaccga gggtgaattt
      301 ttctgcctgc gtgcgggcgg ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag
      361 ctgcccggca aaactaatgg tcggttgcgg cagtgaatcc tgcgcacgcg cactcatcgc
      421 cgtagcctgt aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc
      481 ctggataatt tctggtagca aagcctacca gtaagtcaat aagacaaagg cgctaaatag
      541 caacaaaaaa acgggtttat tggcggatag aaaaaaacga aagcacaaat aatggcagcg
      601 tcaatttttc gagtttgctg acccgggagt gagtcttgtt ccactttgcc aataacgccg
      661 tccataatca gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa aatctcttga
      721 gtggcttaat cgcctgcgtg cgaatccgaa aattccattg attgttgccg gttccgcggc
      781 agtggcggtc atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc gcacattatt
      841 cagcaatctt tccgatcagg atggtggcgc aattgtcagc caactgacgc aaatgaatat
      901 tccttaccgc ttcagcgaag ccagcggcgc tattgaagtt ccggcagata aagttcacga
      961 gctgcgtctg cgcctggcgc aacaaggttt gcccaaaggc ggcgcggtcg gtttcgaact
     1021 actagatcag gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt atcagcgggc
     1081 gctggaaggc gagctttctc gtaccatcga aactatcggc ccggtaaaag gggcgcgcgt
     1141 acatctggca atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc cttctgcatc
     1201 ggtgacggta aatctattac ccggccgcgc actcgatgaa gggcaaatta gcgccattgt
     1261 acatctggtt tccagcgcgg ttgctggtct gccgccgggg aacgtcacgc tggtggatca
     1321 gggtggacat ctgttaaccc tgtccaatac cagcgggcgc gatcttaatg acgctcagtt
     1381 gaaatatgcc agcgatgtcg aaggccggat tcagcggcgt attgaagcga tcctgtcgcc
     1441 aattgttggt aacggtaata ttcacgccca ggtcacggcg caactggact tcgccagtaa
     1501 agaacaaacg gaagaacagt atcgccctaa cggtgatgaa tctcatgcgg cgcttcgttc
     1561 acgccagctt aatgagagcg agcaaagcgg ttccggttat ccgggcggcg taccgggggc
     1621 gttgtcgaat caaccggcac ctgcgaataa cgcgccaatc agcacgcctc cggcaaatca
     1681 aaataaccgc cagcagcagg cgagcaccac cagcaatagc gggccgcgta gcacacagcg
     1741 gaatgaaacc agtaactacg aagtcgatcg caccattcgt cataccaaaa tgaacgtggg
     1801 cgatgtgcaa cgtctgtcag tcgcggtagt ggtaaattac aaaaccttgc cagacggtaa
     1861 accattgcct ctcagcaacg aacagatgaa gcaaattgaa gatctgaccc gcgaggcgat
     1921 gggcttttct gaaaaacgcg gtgactcgct caatgtcgtt aactcgccgt tcaatagcag
     1981 tgacgaaagc ggcggagaac tgccgttctg gcaacagcaa gcgtttatcg atcagttgct
     2041 ggctgccggt cgctggttgc tggtactgct ggtggcgtgg ctgctgtggc ggaaagcggt
     2101 acgcccgcag ttaacaagtc gcgccgaggc gatgaaagct gtacagcaac aggcgcaggc
     2161 ccgtgaggaa gtggaagatg cggtggaagt ccgcctgagc aaagacgaac aactccaaca
     2221 acgacgcgct aaccaacgtc tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc
     2281 tgataacgat ccgcgcgtgg tggcgctggt cattcgccag tggataaata acgatcatga
     2341 gtaacctgac aggcaccgat aaaagcgtca tcctgctgat gaccattggc gaagaccggg
     2401 cggcagaggt gttcaagcac ctctcccagc gcgaagtgca aaccctgagc gctgcaatgg
     2461 cgaacgtcac gcagatctcc aacaagcagc taaccgatgt actggcggag tttgagcaag
     2521 aagctgaaca gtttgccgca ctgaatatca acgccaacga ttatctgcgt tcggtattgg
     2581 tcaaagctct gggtgaggaa cgtgccgcca gcctgctgga agatattctc gaaactcgcg
     2641 ataccgccag cggtattgaa acgctcaact ttatggagcc gcagagtgcc gccgatctga
     2701 ttcgcgatga gcatccgcaa attatcgcca ccattctggt gcatctgaag cgcgcccagg
     2761 ccgccgatat tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtgtcg
     2821 ccacctttgg cggcgtgcag ccagccgcgc tggcggaact gaccgaagta ctgaatggct
     2881 tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa
     2941 ttatcaacct gatgaaaact cagcaggaag aagccgttat taccgccgtg cgcgaattcg
     3001 acggcgagct ggcgcaaaaa attatcgacg agatgttcct gttcgaaaat ctggtggatg
     3061 tcgacgatcg cagcattcag cgtctgttgc aggaagtgga ttccgaatcg ctgttgatcg
     3121 cgctgaaagg ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg
     3181 ccgccgatat tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg tcgcaggtgg
     3241 aaaacgaaca gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact ggcgagatgg
     3301 taattggcag cggcgaggat acctatgtct gataatctgc cgtggaaaac ctggacgccg
     3361 gacgatctcg cgccaccaca ggcagagttt gtgcccatgg tcgagccgga agaaaccatc
     3421 attgaagagg ccgaccccag ccttgagcag caactggcgc aactgcaaat gcaggcccat
     3481 gagcaaggtt atcaggcggg gattgccgaa ggtcgccagc aaggtcatga gcagggctat
     3541 caggaaggac tggcccaggg gctggagcaa ggtctggcaa aggcgaagtc tcaacaagcg
     3601 ccaattcatg cccggatgca gcaactggtc agcgaatttc aaactaccct tgatgcgctt
     3661 gatagtgtg
//
LOCUS       DAJTSL010000255         3565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTSL010000255 DAJTSL010000000
VERSION     DAJTSL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.258"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(90..875)
                     /gene="lafU"
                     /locus_tag="OO433_004010"
     CDS             complement(90..875)
                     /gene="lafU"
                     /locus_tag="OO433_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6052289.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHIDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            847..2559
                     /locus_tag="OO433_004011"
                     /pseudo
     CDS             847..2559
                     /locus_tag="OO433_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2783..3280)
                     /gene="rayT"
                     /locus_tag="OO433_004012"
     CDS             complement(2783..3280)
                     /gene="rayT"
                     /locus_tag="OO433_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6052290.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(3456..>3565)
                     /locus_tag="OO433_004013"
     CDS             complement(3456..>3565)
                     /locus_tag="OO433_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HCR6052291.1"
                     /translation="SPRTGETIRVSRLAEPFWQDHFLGARWILTEETIL"
BASE COUNT          829 a          970 c          947 g          819 t
ORIGIN      
        1 aaatcatgat tttacgcatt gctcacctct caacactggt aaagtataca gtgatttcag
       61 agtttgagaa atgcgtaaag attcagcatt taacgcgttc gctgcgaaag cacctgctgc
      121 ttatccagct tttgcaccag cggctgcacc actttatccc catgctgacc aaagtattga
      181 tacagcgtat cggacgcact tttggtcagc accataatct caatgcgccg gttgcccgcg
      241 ctttgcggat ttttggaatc cagcagcatc tggtccgcca ttgcgcttac ctgcatcact
      301 ttatcttccg gcattccggc ctcttccagc acccgacgag ccgaaagcgc gcggtcaccc
      361 gaaaggttcc agttgttgta gatattgttt ttgtaagcca tcgcatcgat atgcccggta
      421 ataataattt tgttatcgag cgagtcgaaa actggcgcga gctccaccag cagcgtttta
      481 aagaacggca taatctgcgc gctgccgcgt tcgaacatat tgcgattctg gtcgtcttta
      541 atcagcacgc gtaatccctg gggaacaatc tccatctcca gattcgcttc catatgcgca
      601 tcacgggcaa tggtggtgat actggttgcc agctcgccaa gctcggtggc ggatttcttc
      661 ttcagcagcg ccgttttctc attgacgtcg cgcgcttttt tctccgtttc ttccggcacc
      721 gccacggtgg caggttttgg cggatgagac gggctgattt tatttaacgg cgacagccca
      781 ccaccgttaa aaatcgactg cccgtgcagc gcggcgataa tgctttcacg ctcggattta
      841 ctgacgctgt tgacaatcaa ctttatcgtc gtcaccaaag gggccgagcg tatttccgag
      901 gtttcagccc gctttaccct ggatgcgatg cccggtaagc agatggcgat tgacgccgat
      961 cttaatgccg ggttgatcaa tcaggcgcag gcacaaaccc gacgtaaaga cgttgccagc
     1021 gaggccgatt tctacggcgc gatggacggg gcatcgaaat ttgtgcgcgg ggatgctatc
     1081 gccgggatga tgattctggc gatcaacctg atcggcggcg tctgtatcgg gatcttcaaa
     1141 tacaacctga gcgccgacgc cgccttccag cagtatgtgc tgatgaccat cggcgacggg
     1201 ctggtggcgc aaatcccttc cctgctactc tccaccgcgg cggcgattat cctcacccgc
     1261 gtcagcgaca acggcgatat cgcccatgac gtgcgtaacc agttgctggc aagcccatcg
     1321 gtgctctaca ccgctaccgg aattatgttc gtactggcgg tagtgccggg aatgccacat
     1381 ctgccgtttt tgctgttcag cgccctgctt ggctttaccg gctggcggat gagcaaacgc
     1441 ccgcaggcgg cggaagcgga agagaaaagt ctcgaaacgc tgacccgcac catcaccgaa
     1501 accagcgagc aacaggtcag ttgggaaacc attccgctta tcgagcctat cagtttaagc
     1561 ctcggctaca agctggtggc gctggtcgat aaagcccagg gcaacccgct cacccagcgt
     1621 attcgcggcg tgcgacaggt gatatccgac ggcaacggcg tgctgctgcc ggagatccgc
     1681 attcgggaaa acttccgcct taagcccagc cagtacgcta ttttcatcaa cggcattaag
     1741 gctgatgaag cggatattcc ggcggataaa ctgatggccc agccctccag cgaaacctac
     1801 ggcgagatag acggcgtgct ggggaacgac ccggcgtacg ggatgccggt gacctggatc
     1861 cagcctgcgc agaaagcgaa ggtgctgaat atggggtatc aggtgatcga cagcgccagc
     1921 gtgattgcta cccatgtaaa taagattgtg cgcagctata ttcctgattt atttaactat
     1981 gacgatatta cgcagttgca taaccgtttg tcgtcgatgg caccgcgcct ggcggaggat
     2041 ttaagcgcgg tgctcaatta cagccagttg ctgaaagtgt accgggcgct gctgaccgaa
     2101 ggcgtttccc tgcgcgatat cgtcaccatc gccaccgtgc tggtcgccag tagcgcggtg
     2161 accaaagatc atattctgct ggcagccgat gtgcgcctgg cgctgcggcg cagcattacc
     2221 catccgttcg ttcgcaagca ggagctgacg gtgtataccc tgaataatga gctggaaaat
     2281 ctgctgacta acctagtgaa tcaggcgcaa cagggcggta aagtgatgct cgacagcgtg
     2341 ccagtggacc cgaatatgct caaccagttc cagagtacga tgccacaggt gaaagagcag
     2401 atgaaagcgg cggggaaaga cccggtgctg ctggtgccac cgcagctgcg ccctttgctg
     2461 gcgcgttatg caaggttgtt tgcgccgggg ctgcatgtgc tgtcgtataa cgaagtgccg
     2521 gacgagctgg agttgaagat tatgggggcg ttgatgtaag tatccagcat tggtgcacaa
     2581 ctgccggatg cggcgtgaac gccttatccg gcctaccgat ccagcacagg tttgtaggca
     2641 tgataagacg cgccagcgtc gcatcaggca tctgcgcata accgccggat gcggcataaa
     2701 cgccttatcc ggcctaccga tccggcacag gtttgtaggc atgataagac gcgccagcgt
     2761 cgcatcaggc atctgcgcat aattataaaa tgatacgctc gccagcattt atatccgtta
     2821 cgtcccccgc ccaatcgatg ggatataacc ctcgcgcgac atcgcgatgg aacgttgaga
     2881 atggccaatc actcacttgc tttacccaac catgctttac tggatttata taaatataat
     2941 caacatgatg cctataatct ttggtattgc ggatggcgtg ctcccaaaaa cgtggttgcc
     3001 agatattttt caatccacaa gcatgggtaa attgcttttt aatttcccgc cagcgagagg
     3061 aaaaatcatc atcgccttca ggtaatgtcc atatgcaatg catatgctct ggcaaaacga
     3121 cccaggcgtt gatttcaaaa ggcctgtctc gcttaacttt aataatggcg ttacggagca
     3181 tctggtactg ggtggtcaag agttggcttc gacgattgcg taaattcacc gtgaaaaacc
     3241 aggttccccc cttgatgtaa taacgacgat attcagacat ggcgactcct ttttctcaac
     3301 ggtaagaaga aaaggaaagg tcggcaatga ggtgatggaa taacaggaaa gcggctcgca
     3361 aaaacgcaca gatttgtagg catgataaga cgcgtcagcg tcgcatcagg catctgcgca
     3421 ccaccgccgg atgcggcgta aacgccttat ccgtcctaca aaatcgtctc ttccgtcaat
     3481 atccaccgcg cgcccaaaaa atgatcctgc cagaaaggtt cagctaaccg acttacccga
     3541 atggtttccc ccgtgcgcgg cgact
//
LOCUS       DAJTSL010000256         3518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTSL010000256 DAJTSL010000000
VERSION     DAJTSL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.259"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            391..1110
                     /locus_tag="OO433_004014"
     CDS             391..1110
                     /locus_tag="OO433_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6052292.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVGHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            1121..2548
                     /locus_tag="OO433_004015"
     CDS             1121..2548
                     /locus_tag="OO433_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6052293.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKNKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2541..3236
                     /locus_tag="OO433_004016"
     CDS             2541..3236
                     /locus_tag="OO433_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6052294.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
BASE COUNT          921 a          738 c          965 g          894 t
ORIGIN      
        1 tacaaactaa attgcttcaa ttaaataaaa tcccgactat tacttgatac gtgattattg
       61 tcggtgattt tgtttgtaca ccatcagtgt atatctcaat aacccctgaa taagtagctc
      121 tgaataggta taagggatgt ggccattttt aaatggattt cttatgccaa aaatgatcgt
      181 cgatttctcc ataaaatgtg agcgatgccg aaagaaataa aattagttat tgcatttgac
      241 gtttgggtga aagattatca tttgtcatac aaatgagggg tagtatgttg ctagccaatt
      301 aaaaaagaac gccatattta ttgatgattg atcccccggt gggggggaaa catcgggcat
      361 aaatgggcat gaagtaatgg agtattagtt gtgaatgtca atttctttgt cacctgtatt
      421 ggtgacgccc tgaaatcaag aatggcacga gactccgtgc tgctactgga aaaactcggc
      481 tgtcgcgtaa atttcccgga gaaacaggga tgctgcggtc agcctgcgat caatagcggt
      541 tatatcaaag aagcgattcc agggatgaaa aatctgatcg ccgcactgga ggataacgac
      601 gatcccatta tttcaccggc tggctcttgc acctatgccg taaaaagtta cccgacgtat
      661 ctggcggatg aacctgaatg ggcatcacgt gccgaaaagg ttgccgcgcg tatgcaggat
      721 ctcacctctt ttattgttaa taaattaggg gtagtcgatg taggtgccag tttgcaaggg
      781 agagctgtgt atcacccatc ttgtagcctg gcccgtaagc tgggagtgaa ggacgagcca
      841 cttacgctgc tgaaaaatgt gcgtggactg gagctgttga cctttgctga acaggatacc
      901 tgctgcggat ttggcggcac gttctcggtc aaaatggccg aaatatccgg cgagatggtg
      961 aaagaaaagg ttgggcacct gatggaagtc cgccctgagt atttaattgg tgctgacgtg
     1021 agttgcctgc tgaacatcag tgggcgatta caacgggaag ggcagaaagt caaagtgatg
     1081 catattgctg aagtgttgat gagccgctga ggatataaag atgtcgatca aaaccagtaa
     1141 tacagatttt aagactcgca tccgtcagca aattgaagat ccgatcatgc gcaaagcggt
     1201 ggcaaacgcg cagcagcgta ttggggcaaa tcggcaaaaa atggtcgatg aattggggca
     1261 ctgggaggag tggcgcgatc gggccgccca gatacgtgat catgttctga gtaatctcga
     1321 cgcttatctg taccagctct cagaaaaagt gacgcaaaac ggcggtcacg tctggtttgc
     1381 aaaaaacaaa gaagacgcta cccgctacat tttacaggtt gcccaacgca aaaatgcccg
     1441 gaaggtggtg aaatctaaat cgatggtgac cgaagagatt ggtgtcaatc atgtgttgca
     1501 ggatgctggc attcaggtga ttgaaaccga tctgggtgaa tatattctcc agctggatca
     1561 agatccgcca tctcatgttg tggtcccggc aattcataaa gatcgccatc agatccgtcg
     1621 agtgctacac gaacgtctgg gctatgaggg gccggaaacg cctgaagcga tgaccttatt
     1681 catccggcaa aaaatccgcg aagatttcct cagtgctgaa ataggtatta ccggctgtaa
     1741 tttcgcggtg gcagagacag gttcggtatg cctggtgacc aatgaaggta atgcgcgaat
     1801 gtgtaccacg ctgcctaaaa cgcatattgc agtgatggga atggagcgta ttgcccccac
     1861 gtttgccgag gtagatgtat tgatcaccat gctggcgcgc agtgccgttg gtgcacgttt
     1921 gacgggatac aacacctggc tgacaggacc gcgcgaagcg gggcacgttg atggtcctga
     1981 agagtttcat ctggttattg tcgataacgg gcgttctgag gtgctggcct ctgaatttcg
     2041 ggatgtgctg cgctgtattc gctgcggggc ttgtatgaat acttgtccgg catatcgcca
     2101 tattggcggt catggatatg gctctattta tccagggcca attggtgcgg tgatttctcc
     2161 gctacttggc ggctataaag attttaaaga tttaccctac gcctgctctt tatgcactgc
     2221 ttgtgacagc gtgtgtccgg tgcgtattcc gctgtcaaaa ctgattttgc gtcatcgtcg
     2281 ggtgatggct gaaaaaggga tcaccgcaaa agcagagcaa cgggcgataa aaatgttcgc
     2341 ttatgccaat agtcatccag gattgtggaa agtcgggatg atggccggcg ctcatgcggc
     2401 aagctggttt atcaatggcg gcaaaacacc actcaaattt ggcgcgatta gcgactggat
     2461 ggaagcacgc gatcttcctg aagctgacgg agagagtttc cgtagttggt ttaagaaaca
     2521 tcaggcgcag gagaaaaaga atggataatc gaggcgaatt tttgaataac gttgctcagg
     2581 cactgggtcg cccgctgcga cttgaaccgc aagcagaaga tgcgccgctt aacaactatg
     2641 ctaacgagcg gcttacccaa cttaaccaac agcagcgctg tgacgcgttt attcagtttg
     2701 ccagcgatgt tatgttgacg cgctgtgagc tgaccagcga ggcgaaggcg gcagaagctg
     2761 caatacgtct gtgtaaagag ctgggagatc agtcggtcgt gattagcggt gacacgaggc
     2821 tggaggaatt ggggattagt gaacgtttgc agcaggaatg caatgccgtt gtttgggatc
     2881 cggcgaaagg tgccgagaat atctcgcagg cagagcaggc taaagtgggt gttgtgtatg
     2941 ctgaatatgg tttaaccgaa tcgggaggcg tggttctttt ttccgccgcc gagcgcgggc
     3001 gttcattgag cctgctcccg gaatcttctc tttttatcct gcgtaaaagc acaatcctgc
     3061 cgcgtgtagc gcaactcgca gaaaaattgc atcagaaagc gcaggccggt gaacgaatgc
     3121 cgtcctgcat taacatcatt agcggcccta gttcaacggc ggatattgag cttatcaaag
     3181 tcgtcggggt tcatggcccg gtgaaagcgg tgtatctgat tattgaggat tgctgagaga
     3241 gatatgagat ctgaacataa acctgaagaa ggcatacgag gttgaaaata gcgttaagga
     3301 caacctgact taaccccgtt ccgtcatggt tatgctgctc ttctgtaaat atcctggcat
     3361 tagtaatgcc aggatattta cagtgaaaaa gacaggcatc cattcctgaa aacgggttcc
     3421 cttcccggtc attgtcactt cagctttata caggcactct ctatcagaat gttgtttatt
     3481 actgcccaga gttttgttgg tattcatcta ccattttt
//
LOCUS       DAJTSL010000257         3481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTSL010000257 DAJTSL010000000
VERSION     DAJTSL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.260"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..1946
                     /locus_tag="OO433_004017"
                     /pseudo
     CDS             1..1946
                     /locus_tag="OO433_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            complement(1989..2435)
                     /gene="ivy"
                     /locus_tag="OO433_004018"
     CDS             complement(1989..2435)
                     /gene="ivy"
                     /locus_tag="OO433_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6052295.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            2616..3386
                     /gene="yafV"
                     /locus_tag="OO433_004019"
     CDS             2616..3386
                     /gene="yafV"
                     /locus_tag="OO433_004019"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6052296.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
BASE COUNT          777 a          891 c          999 g          814 t
ORIGIN      
        1 tgattatcaa aaaagagtac ggctgactgg agttctcagc ttatgcccag tctcgcgtgc
       61 tgcaaaaact ctccggcgtg agcgggatcc tggcgattac cgtcggcgtg ccgaactcat
      121 taggcccggg cgagctgctg caacattacg gcactgacga gcagaaagat cactatctgc
      181 cgcgtctggc gcgtggtcag gagatccctt gctttgcact gacaagcccg gaagcgggtt
      241 ccgatgcggg cgcgattccg gacaccggga ttgtctgcat gggcgaatgg cagggccagc
      301 aggtgctggg gatgcgtctg acttggaaca aacgctatat tacgctggca ccgattgcga
      361 ctgtgcttgg gctggcgttt aaactctccg acccggaaaa gctgctgggt ggcgcagaag
      421 atttaggtat tacctgtgcg ctgatcccga ccaccacgcc gggcgtggaa attggtcgtc
      481 gccacttccc gctgaacgta ccgttccaga acggaccgac gcgcggtaaa gatgtcttcg
      541 taccgatcga ttacatcatc ggtgggccga aaatggccgg gcaaggctgg cggatgctgg
      601 tggagtgcct ctcggtaggc cgcggcatca ccctgccttc caactcaacc ggcggcgtga
      661 aatcggtagc gctggcaacg ggcgcgtatg ctcacattcg ccgtcagttc aaaatctcta
      721 tcggaaagat ggaagggatt gaagagccgc tggcgcgtat tgccggtaat gcctacgtga
      781 tggatgctgc ggcatcgctg attacctacg gcattatgct cggcgaaaaa cctgccgtgc
      841 tgttggctat cgttaagtat cactgtaccc accgcgggca gcagtcgatt attgatgcga
      901 tggatatcac tggtggcaaa ggcattatgc tcgggcaaag caacttcctg gcgcgtgctt
      961 accagggcgc accgattgcc atcaccgttg aaggggctaa cattctgacc cgcagcatga
     1021 tgatcttcgg acaaggagcg attcgttgcc atccgtacgt gctggaagag atggaagcgg
     1081 cgaagaacaa tgacgtcaac gcgttcgata aactgttgtt caaacatatc ggtcacgtcg
     1141 gtagcaacaa agttcgcagc ttctggctgg gcctgacgcg cggtttaacc agcagcacgc
     1201 caaccggcga tgccactaaa cgctactatc agcacctgaa ccgcctgagc gccaacctcg
     1261 ccctgctttc tgatgtctcg atggcagtgc tgggcggcag cctgaaacgt cgcgagcgca
     1321 tctcggcccg tctgggggat attttaagcc agctctacct cgcctctgcc gtgctgaagc
     1381 gttatgacga cgaaggccgt aatgaagccg acctgccgct ggtgcactgg ggcgtacaag
     1441 atgcgctgta tcaggctgaa caggcgatgg atgatttact gcaaaacttc ccgaaccgcg
     1501 tggttgccgg gctgctgaat gtggtgatct tcccgaccgg acgtcattat ctggcacctt
     1561 ctgacaagct ggatcataaa gtggcgaaga ttttacaagt gccgaacgcc acccgttccc
     1621 gcattggtcg cggtcagtac ctgacgccga gcgagcataa tccggttggc ttgctggaag
     1681 aggcgctggt ggatgtgatt gccgccgacc caattcatca gcggatctgt aaagagctgg
     1741 gtaaaaacct gccgtttacc cgtctggatg aactggcgca caacgcgctg gcgaagggcc
     1801 tgattgataa agatgaagcc gctattctgg tgaaagctga agaaagccgt ctgcgcagta
     1861 ttaacgttga tgactttgat ccggaagagc tggcgacgaa gccggtaaag ttgccggaga
     1921 aagtgcggaa agttgaagcc gcgtaattga ataacggagc cgaaaggctc cgtttcttta
     1981 tccgctaatt atttaaaatt aaagccatcc ggatggtttt ccaggctgcc ggtcaacgcc
     2041 gcgaacagca ccgttttacc atcaatcgaa agcgcatcgt tcacattcag ccaggtgagt
     2101 ttctcttgcg acgttttctc atcaatagtc gagaacagcc ccgtcatctg attagatttc
     2161 tcggaccaca tcacagcgat acgttgcgag ccacagtcat gcggtttgca cgcgctcatc
     2221 acctgatacg tctcatctcc caacgttacg gtttgtgcgg gagtataagt accgcctttc
     2281 atcacccagg caggcagctt atgcccttgt accatctgat taaatgcagc tttggtggtt
     2341 tcgccctttg caaggctgct aatggttaaa tcatcctgcg ccattgcact ggtggcgatg
     2401 accagagcgg cgactgtcgt tattgcctta aacatcattc ctcccgagct tatcctgccc
     2461 atgttattaa ccttctaaag cctggcgata acacgataat ttgcaaatca gatatttcca
     2521 atatcagcat ttgttatcaa tttattctgc gctaaggcaa ctgcctgata aggtgttaaa
     2581 gttataaaac ccttcgcata aaggagttgt acaccgtgcc tggtttgaag attacgcttt
     2641 tgcagcaacc actggtgtgg atggatggcc ctgccaacct gcgtcatttt gatcgtcaac
     2701 tggaaggtat taccgggcgc gatgtgatcg ttctaccgga gatgtttacc agcggctttg
     2761 ccatggaagc ggcagcttcg tcgctagcac aagatgacgt agtgaactgg atgacagcta
     2821 aggcgcagca gtgcaatgcg ctgatagcag gcagtgttgc attacaaacg gagtctggtt
     2881 cggttaaccg ctttttgctg gttgagccgg gcggcacggt acatttttat gataagcgtc
     2941 atctgttccg catggcagat gagcatctac attataaagc gggcaatgcg cgagtgattg
     3001 tggaatggcg cggctggcgt attttgccgt tggtgtgcta cgacttacgt tttccggtgt
     3061 ggtcgcgcaa tctcaacgat tatgacctcg ccatctacgt tgccaactgg cctgctccgc
     3121 gctctctgca ctggcaggca ttgctgacgg cccgcgcgat tgagaatcag gcgtatgtgg
     3181 cgggatgcaa tcgcgtcggc agcgatggca acggctgcca ttatcgcggt gacagccggg
     3241 tgattaatcc acaaggcgag attatcgcta ctgccgacgc gcatcaggca acgcgcattg
     3301 atgcggagct gtcgatgatg gcattgcggg aatatagaga aaagtttccg gcatggcagg
     3361 atgcggatga gtttaggttg cggtgagtgg gggttgtgtt taaggctggg ggagagtcgg
     3421 ggtaagacta cgaaagcccg ctctccgcaa ggactgacgc cagatagttt ctgtccatag
     3481 c
//
LOCUS       DAJTSL010000258         3399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTSL010000258 DAJTSL010000000
VERSION     DAJTSL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.261"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(9..311)
                     /locus_tag="OO433_004020"
     CDS             complement(9..311)
                     /locus_tag="OO433_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052297.1"
                     /translation="MLEIVLSPVKAQQFTVTLDAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEELI"
     gene            complement(311..1021)
                     /locus_tag="OO433_004021"
     CDS             complement(311..1021)
                     /locus_tag="OO433_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052298.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FIVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(1018..3039)
                     /locus_tag="OO433_004022"
     CDS             complement(1018..3039)
                     /locus_tag="OO433_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6052299.1"
                     /translation="MAQVLDELVLSLGIDDRDFTAGEQAVHAALNRLTTVMEGVADTF
                     SQGQKKTSESLEKTGKDADKTARRMEDAGKRASRFFSGIRSEILALAGVSLTLGGLKN
                     LVTGFARDLNRLSVESDAFGMKARNLDGWLRAARANNVDEGEMSGAFSRLANAKAAFR
                     AGRSFDPVLQDLFQVAARAGISVDLNTDSTEAIMRKLAVIFPRLTKSEQTAYGNALGF
                     SYAGQQFLGSGHALKDVDEFTSRSQVTPGRTALARKLRKELVELDQTWSGIGMTISTA
                     LMPYALKLSHWLETLGDWMQQHPEEVNRFITTFLNDVESVASLANKAAGELGGWQNVI
                     ITLIGLKVASWVRGLTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDWLDSHGFF
                     WASDGTFFFNKKEMETYQTKLDAGEKPGNITRAQSPTVWQQGMLDTQASQATGREAAF
                     GESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPPGLLRSVA
                     ITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKLGGLIRFF
                     HGDLAKALAAYNWGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIRSLHPAPV
                     SQSTVTETTHIGTLNVTTTSDNVKGITDDARRRITNSALVSVYSSGVTG"
     gene            complement(3023..3205)
                     /locus_tag="OO433_004023"
     CDS             complement(3023..3205)
                     /locus_tag="OO433_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6052300.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
BASE COUNT          795 a          991 c          814 g          799 t
ORIGIN      
        1 tctgtagctc atatcagttc ctcaggtaac agccagtaga gccggtaccg ggatccgagc
       61 ccccgccagt cagggtcact attcccctcc gtgtcggtaa aaaacaggtc cccctgaaac
      121 ggcaggtatc cgtaccggac aatccggtta ttattcagac acagcacgcc atacaggcac
      181 ggttcaccat taacaatgat atcgatatac atccccgtag tacgctgatt caggcgaatg
      241 gtgcagacct gagcgtccag tgtcaccgta aactgctggg ctttaacggg agataaaaca
      301 atttccagca tcaggtgatc cccctgttcg tggcacttcg tctgtcagcg tctgacggtt
      361 gtgtcaccga cgccgtaact ggctgcgttt taaccgatgc ggcccctttt gctttatcgt
      421 tgtccgtggg agacgactta tccgtacttc ccactgacac ctctcctgta ttcattacgg
      481 tctgaaatac cgcagtaacc gtcagtaagg tcggaccatt attacttcgc attcggtagt
      541 catatttcac aaggtcgcag gatgtccatg tcttgtccgg cgtctcaata tcgtagagtc
      601 ctgccgtggt acgcatccgt tcaagcgttt ccagcacatt cgtccgcgag gtggtggaaa
      661 aatttgtcag gttcgggacg gatccggaaa aggccgtcca cccctctaca atgaaagtca
      721 catgcagttc cggcgatctc tggattttat taaaggtggt ataagccccc tgctccacgg
      781 ggacggtgga aacagaagcc tccgcgccca cctcaacgac aacaaaagaa tccggggaga
      841 aaggtttgcc acccttctga tgggcacctg ccggatcatt ccgtgcgtaa taaataccga
      901 atgacggtgc cagtacacta ttaatgagtc ccaggacacc gccgccacga actgcactca
      961 gtacgttact ttcactgagc gaaaagttat tcagggaaat attatcgaaa gaaaaactca
     1021 tcctgttacc ccgctggaat aaaccgacac aagcgccgaa ttcgtgatac gccgacgtgc
     1081 gtcatcggta atacccttca cattgtccga cgttgtggtg acattcagtg tcccgatatg
     1141 cgtggtttcc gttacggtgg actgcgatac aggcgcagga tggagcgacc gtatggccat
     1201 tgccgcaccc ggataaggca ggttcgccag cacccgcgga atatagttgc gggtctcctc
     1261 cggagcagca gccagtccct tacgctgaac atttccctct ccccagttat atgccgccag
     1321 agccttagcc agatcgccat gaaaaaaacg tatcaggcca ccaagttttc tcgcggcggc
     1381 atcagcggct ttttcgggat caaaggcatc gttccccctc agaccaaatt cactggccgt
     1441 cttcggcatg aactgaaaca gtcccatcgc accagcaggt gagacggcaa actgattacc
     1501 acgggattcg gtgatcgcaa cgctgcgcag cagtccgggc ggcaggttat atttttcctc
     1561 cagttgagac agtttcggtt gcagccagcc taacagggct tccccggtct tcgtcggtcg
     1621 ctggcggttt tgcatggcat taccgagttt ttcctgcgtc gcacgcatac cctgtagcca
     1681 ggattcaccg aaggctgctt ccctcccggt tgcctgagaa gcctgagtat ccagcatgcc
     1741 ctgctgccat actgtgggtg attgtgcccg ggtgatgttg ccaggctttt ctccggcatc
     1801 cagttttgtc tgatacgtct ccatctcttt cttattgaaa aagaaagtcc cgtctgaagc
     1861 ccagaaaaaa ccatgtgaat ccagccagtc cttattttcc ctgccaacaa tggatgtcag
     1921 taatccgtca acaaccgggt aaagcgccgt tatcgcaaaa agaaggcctc cggggccact
     1981 gagggctttt gtcagccccc gcacccatga cgccactttc agcccgatca gcgtaataat
     2041 gacattctgc cagcccccca gttctccggc agccttattc gccagggagg ccactgactc
     2101 aacgtcattc agaaatgtgg tgataaacct gttcacttcc tccggatgtt gctgcatcca
     2161 gtcaccgagt gtctccagcc agtggctgag cttcagcgcg tacggcatca acgcggtgct
     2221 aatcgtcatc ccgatgccgg accaggtctg atcgagctca acaagctctt ttcgtagctt
     2281 acgggccagc gctgtacgcc ccggcgtaac ctgcgaacgg gaagtaaatt cgtccacatc
     2341 tttcagcgca tgaccggagc caagaaactg ctgtccggca taactgaagc ccagcgcgtt
     2401 gccgtaggca gtttgttcgg actttgtcag tcgcggaaaa ataaccgcca gcttgcgcat
     2461 gatggcttca gtgctgtcag tatttaaatc aacactaatg cccgccctgg ctgcaacctg
     2521 aaacaaatcc tgtaacacag gatcaaagga ccttccggct ctgaaggccg cttttgcatt
     2581 tgcaagccgg gaaaatgccc cagacatttc cccttcatca acattatttg cccgcgcagc
     2641 ccgtaaccat ccgtccagat ttctggcctt catgccaaaa gcatcggatt ccacggacag
     2701 acggttcaga tcacgggcaa aacctgttac caggttcttc agccccccca gcgtcagact
     2761 gacacccgca agagccagaa tctcgctacg gatccctgaa aaaaaacggg acgcccgttt
     2821 cccggcatct tccattcgac gggccgtttt atcagcatct tttccggttt tttccagtga
     2881 ttcactggtt ttcttctgtc cctgactgaa tgtgtcagcg acaccttcca tcaccgtcgt
     2941 gagccggtta agggcagcat gtactgcctg ttccccggca gtaaagtccc tgtcatcaat
     3001 cccgagggac agaacaagct catcaagtac ctgcgccatt tctgttctcc tgtatcacct
     3061 gttcgttatg ggcatccaca tgaattattt ccagcagatc ccataagtcc tgcacaccaa
     3121 ggacagaatc cagttcagct tttgaagcct taccggagga gataacggtc gcaatggtac
     3181 ggggaacatt aacgtaatcc accaccccga acggtctgtc ggggtcgaga taacgcgggg
     3241 ggatatctag ctgacgacgg tagcgaaaaa atccacatgc agcttaaaga cctccgcacg
     3301 cagattaaga cgggtaatga tttcttcaat atcctcttca acaagcggtc tccggatgct
     3361 gcgttttttc ggatcgggaa caaactgcac acagtccat
//
LOCUS       DAJTSL010000259         3320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTSL010000259 DAJTSL010000000
VERSION     DAJTSL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.262"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..212)
                     /locus_tag="OO433_004024"
     CDS             complement(<1..212)
                     /locus_tag="OO433_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052301.1"
                     /translation="MIRFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVCISLQELQLELMVEYALFF"
     gene            complement(215..982)
                     /locus_tag="OO433_004025"
     CDS             complement(215..982)
                     /locus_tag="OO433_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052302.1"
                     /translation="MQPCPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESLGK"
     gene            complement(985..1695)
                     /locus_tag="OO433_004026"
     CDS             complement(985..1695)
                     /locus_tag="OO433_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052303.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1711..3216)
                     /locus_tag="OO433_004027"
                     /pseudo
     CDS             complement(1711..3216)
                     /locus_tag="OO433_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
BASE COUNT          935 a          798 c          728 g          859 t
ORIGIN      
        1 ctgaaaaaaa gtgcatattc aaccattaac tcaagttgta attcctgaag agaaatacac
       61 acaaccagga gcggaaagga gaatattatt tctgccagaa aagttaacgt gaatataagt
      121 acggctaaga caataaccgt cggccatacc aataatggca cgataacaaa accgagaatt
      181 acagggggaa tataaagccg gaatctaatc atttttattt tcctagtgat tcggatgatg
      241 cgtcatacat cgcgcataaa cactcatcaa ttatccagtc atcttcccag acctgatatt
      301 ctgtcattgg ttcgggctgt tttagcgggt tctcaatcgc ttgccgcgct ctttctttca
      361 gtaaaggatg attatttcta gcccaggcat tgatttgggt ggtaatgacg ttatcgatat
      421 cgtcctggtt atctctgata taatcctcac aatatctgtt caataaacag gcaagagaaa
      481 cggtcgacca aaagccggga actgaattaa tggcatccag caatggcgca agagcgatat
      541 cggcacggaa aaacatttcc agcatatact gtaattcgac agcttcatcg ccggataccc
      601 aatcagtcat atactggcag gcaaatcgct gtaaaattgc ccgttcatcc gcacgccagt
      661 tttctttgtt ggcaaggttg atacggcgca gagaaaaaac tacgtgaata ttgtcaaaat
      721 gcccctgtgc aacaaattcc agtaaacgcg gcagaaaata gcggacctca tcgctatttt
      781 gcggatcagg ccctgggcta ctattccagg cattgattaa ggaatacggg atcgctcgta
      841 acgatgtact ccgtaatgcc ttttgttcct gctgactcag gcaatattca caacaaacca
      901 caaatttctg cggcacccga taacgacgaa aggtgtgata aatctgctca attaatgcct
      961 gcatggcggg gcagggttgc atagttaccc cctcgcccgc tgatcgcgtg taaacggttg
     1021 atagacaaaa cgagcattcg ccgcgttcag gcgaataagg gcgctacgcg aaagggaaaa
     1081 gctggggcga atgcaactat tgcagtctcg acagctttcc tctgcgcaat tttccactga
     1141 gtaatgacgc gtgtagagtt tcagttcggc ctgcttatcg cgaaatccgc gtaaactgtt
     1201 attgttatga aatttccagg catctttaaa gacctgaaag atatagacat cctcaatcca
     1261 ggagtcacat tttttaagcg ggcgctttgc tattatcacc cgctcatccg gatttagcat
     1321 cgccgtaaat tgtacggtga cgtcatttgt cgtgttcagg tcacattgaa catctggtac
     1381 atcactgata taggggtcaa atccaaaatc agcaccacag gcacaaagac ggctaatggt
     1441 ggcggtatcg agggaaaatc ccggccatac tttgctggag gttaagccaa tatttaatgt
     1501 gacatcatat cgaagcaatc cctcaagacc gtcagcaccg acgaactttt ccccttcatc
     1561 cagggcctgg ttaataagtt gctgaacaat gccttcatcc cagtatgccg atttgcagtt
     1621 ggcaatcgta atatccacgc ctgtgaaggg atcagtaccg gcaaggcttg caaataatgt
     1681 attaactaat tccattttta ttatccagct ttaaggtagc gtcttataca tcgggacata
     1741 acctacatct tccaccaaac tctgtccctg cggcgttaaa aaccactcca acaatttttg
     1801 tgtctctgac gtcgtatttt cccgcgttac cataaaggca tcgatgatat agggatattt
     1861 gccgttgcga atgttttcgg cagtcggtgc aataccgtta atcgctagca gttttatatt
     1921 tttatcggcg ttcatttgtg ttgcgtaata acggaaggtg taacctattg cgttgtttgt
     1981 attacggtat tcggcaacga ctttaatcat tccctccatc atacttgcca cttctgtttc
     2041 ctgcggcgag atcatgcgga catttttcat gacctgggat tgcatcaccg tctggctacc
     2101 agagtcttcc gggcgttgct aggtctggat ctcctgatcg ttaccgccaa cagtacgcca
     2161 gttggtaatt gcaccactga agatgtcacg cacctgttgt tcagtcaggg aattaaccgg
     2221 attatccgca ttgacgatga aaacaaacgc ttcacgggca aaaggcgtgt acagcaaagt
     2281 aatgcccgat tcctcagcgc gttttttctg cccaccggaa ggttgcgcca caaaaataat
     2341 atcagcatca cctttaacaa tcctgttata cgcatcgggg gtacgagagc tctccagata
     2401 ttccctggtg tgaaaatctt ccggtataac gctcaaagca taaaatgcag aggcataaat
     2461 tgggtacgcc gccgtagcac catcgagtcg cggccagttt tgcgtaaact gaatttgcgg
     2521 tttaccacgc agtggtgtca gttgattatt gagtttatcg ggtcgccagg cccaggtatc
     2581 aatattttcg cgcacaccga cgccgggatt gagctgttta tataaataac cctgataacc
     2641 agcgacaaaa ccaagcatga gaaggataaa caggagggaa ttacggcaac gtagcccgga
     2701 tgtaccggtt aaaggatgtc tggttggccg gccgtacccc agcgaaaaac tgatatgcgc
     2761 gccaacgggt ataatgacta tccaccagta ctcacctccg actactgaga ctaacaaaac
     2821 gcccagccag gggagcataa gatcagccaa ccaacccgat cccatctggt gaaattgata
     2881 atctgccaga tgcagcgcga ttatccagcc aaccaggatt aataaaccgg gtactaaaaa
     2941 aagacattgt cgccagacgg ctatttctct tttttctggt gcactatcgc ctgcccatac
     3001 tcgccccgag aaaaatagca ttaatgcgcc cagcgccagc ataaaaatgt tgagcagaag
     3061 gtaacttgtt ggcgtgataa ggatattaca aacttcaata ataccggcaa gtccgacacc
     3121 cagcatagca acaaccgccg ccaaaaattg cgtcagtatg gggatgccga aaaaagtcat
     3181 taccagcgag gacaaaatcc attttctgtt ttgcattatt ctttccattc tttttgaatg
     3241 gtgaaattat actccccgag tccccttgcc ccttctggac acttttccga aatgatggcg
     3301 gaaaaaaacg ggaccctttg
//
LOCUS       DAJTSL010000260         3204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTSL010000260 DAJTSL010000000
VERSION     DAJTSL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.263"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..897
                     /locus_tag="OO433_004028"
     CDS             <1..897
                     /locus_tag="OO433_004028"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6052304.1"
                     /translation="DVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWL
                     LSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNIS
                     NAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCVKVR
                     DRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSD
                     ISGENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEY
                     TNNPDLSIANVISLVENAERLN"
     gene            864..1823
                     /gene="psuG"
                     /locus_tag="OO433_004029"
     CDS             864..1823
                     /gene="psuG"
                     /locus_tag="OO433_004029"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6052305.1"
                     /translation="MVSGERRTSELKISPELLQISPEVQDALKNKKPVVALESTIISH
                     GMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVS
                     RRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQEL
                     ANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDS
                     ASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKES
                     TLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            1917..3167
                     /locus_tag="OO433_004030"
     CDS             1917..3167
                     /locus_tag="OO433_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6052306.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
BASE COUNT          765 a          721 c          867 g          851 t
ORIGIN      
        1 gatgtcgccg gatattcaca tgaatcatta aattatgcgg attcaaatcc aggtaaaata
       61 aaatttacgc ctggtggagt cgggcgcaat attgcacaaa acctggcgtt gctggggaac
      121 aaagcctggc tactgagcgc cgtaggcagt gacttttatg gtcaatcgct gctaacgcaa
      181 accaatcaat ctggcgttta tgtcgataaa tgcctgattg tgccgggaga aaatacgtct
      241 agttatttat cattactcga taataccggt gaaatgctgg ttgctataaa tgacatgaat
      301 attagcaacg ctattacagc tgaatatctc gcacagcacc gtgaatttat tcagagggca
      361 aaggtcattg tcgcggactg taatatcagt gaagaggcac tggcatggat tctggataat
      421 gccgccaacg tacccgtatt tgtcgatccg gtttccgcat ggaaatgtgt caaagtgcgc
      481 gaccgtctaa atcagatcca cactctgaag ccaaaccgcc ttgaagcgga aaccctgagt
      541 gggattgcgc tgtcagggcg tgaagatgtg gcaaaagttg ctgcctggtt ccatcaacat
      601 ggcctgaacc gactggtatt gagcatgggc ggcgacggcg tttattacag cgatatcagc
      661 ggtgaaaata gctggtctgc gccgatcaaa accaatgtta ttaatgttac cggagcgggc
      721 gatgccatga tggcgggact tgcttcgtgt tgggtagacg gaatgccgtt tgccgaatct
      781 gttcgtttcg cacagggatg ttcgtcaatg gcgctctcct gtgaatacac caataacccc
      841 gatttatcga ttgccaacgt tatatcgtta gtggagaacg cagaacgtct gaattaaaaa
      901 tttcccctga attattacaa atttccccgg aagtgcagga cgctttaaaa aacaaaaaac
      961 cggttgtggc gctggaatcg accattattt ctcacgggat gccgttccca caaaatgccc
     1021 agaccgcaat tgaagtagaa gaaactattc gtaaacaggg cgctgtacct gccactatcg
     1081 ccattattgg cggcgtgatg aaagtgggtt taagcaaaga agaaattgaa ttactgggtc
     1141 gtgaagggca taacgtgacc aaagttagtc gtcgcgattt accttttgtt gttgccgccg
     1201 gaaaaaatgg cgcaaccact gtggcttcaa cgatgattat tgcggcgctt gccggaatta
     1261 aagtatttgc caccggggga attggtggtg tgcatcgcgg ggcggaacat accttcgata
     1321 tttctgccga tttgcaagaa ctggcaaata ctaatgtcac cgttgtttgt gccggggcga
     1381 aatctattct cgatttagga ttaaccactg agtatttaga aaccttcggt gtgccgttaa
     1441 ttggctatca gactaaagcg ctgcctgcgt ttttctgccg taccagctcg tttgacgtca
     1501 gtattcgtct cgacagcgcc agtgaaattg cccgtgcaat ggcggtgaaa tggcaaagcg
     1561 ggctgaacgg tggcctcgtg gtagcgaacc cgatcccgga acagtttgcg atgccggagg
     1621 aatctatcaa tgcagccata gatcaagccg tcgccgaagc cgaagagcag ggcgttattg
     1681 gtaaagaaag tacactgttc ctgctggcgc gcgttgctga gctgaccggc ggtgacagcc
     1741 tgaaatccaa tatccagctg gtattcaaca acgccattct ggcgagtgaa attgccaaag
     1801 aataccagcg tctcgcgggt taatttccga cgggcagaaa gccgtcgccc gtactcatca
     1861 agcataccaa aacctggcgc tgacgccggg tttactggca tgaagggaaa atcattatgg
     1921 atataatgag aagtgttgtg gggatggtgg tgttactggc aatagcattt ctgttgtcag
     1981 tgaataaaaa gagcatcagt ttgcgcacgg ttggagccgc actgctgctg caaatcgcta
     2041 ttggtggcat catgctctac ttcccaccgg gaaagtgggc agtagaacag gcggcattag
     2101 gcgttcataa agtgatgtct tacagtgatg ccggtagcgc cttcattttt ggttcgctgg
     2161 ttgggccgaa aatggatgtc ctgtttgacg gtgcgggttt tatcttcgcc tttcgcgtac
     2221 ttccggcgat tattttcgtt actgcgctca tcagtctgct gtactacatt ggcgtgatgg
     2281 ggctgctgat tcgcatcctt ggcagcattt tccagaaagc tcttaacatc agcaaaatcg
     2341 aatcttttgt cgcagtcaca actattttcc tcgggcaaaa tgagatcccg gcgatcgtta
     2401 aaccgtttat cgatcgcatg aatcgcaacg agttgtttac cgcaatttgt agcgggatgg
     2461 cgtccattgc tggttcgatg atgattggtt atgccggaat gggcgtacca attgactacc
     2521 tgttagcggc atcgctgatg gcgatcccag gtggtatttt gtttgcacgt attcttagcc
     2581 cggccaccga gccttcgcag gtcacatttg aaaatctgtc gttcagcgaa acgccgccaa
     2641 aaagctttat cgaagcggcg gcgagcggtg cgatgaccgg gctgaaaatc gccgctggtg
     2701 tagcgacggt ggtaatggca tttgtcgcaa ttattgcgct gatcaacggc attatcggcg
     2761 gaattggtgg ctggtttggt ttcgccaatg cctctctgga aagtattttt ggctatgtgc
     2821 tggcaccgct ggcgtggatc atgggtgtgg actggagtga tgccaatctt gcgggtagcc
     2881 tgattgggca gaaactggcg attaacgaat tcgtcgctta cctgagtttc tccccatacc
     2941 tgcaaacggg cggcacgctg gaagtgaaaa ccattgcgat tatctccttt gcgctttgtg
     3001 gttttgctaa ctttggttct atcggtgttg tcgttggcgc attttcggct atttcgccaa
     3061 aacgcgcgcc ggaaatcgcc cagcttggtt tacgggcgct ggcagcagca acgctttcca
     3121 acctgatgag tgcgactatt gccgggttct ttattggttt agcttgagtt tcattgccgg
     3181 atgtacgcgc ttattaagcg ttgc
//
LOCUS       DAJTSL010000261         3194 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTSL010000261 DAJTSL010000000
VERSION     DAJTSL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.264"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            198..938
                     /locus_tag="OO433_004031"
     CDS             198..938
                     /locus_tag="OO433_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6052307.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(909..1676)
                     /locus_tag="OO433_004032"
     CDS             complement(909..1676)
                     /locus_tag="OO433_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6052308.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1882..2460)
                     /gene="lpcA"
                     /locus_tag="OO433_004033"
     CDS             complement(1882..2460)
                     /gene="lpcA"
                     /locus_tag="OO433_004033"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6052309.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2700..>3194
                     /gene="fadE"
                     /locus_tag="OO433_004034"
     CDS             2700..>3194
                     /gene="fadE"
                     /locus_tag="OO433_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="functions in fatty acid oxidation; converts
                     acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCR6052310.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFF"
BASE COUNT          764 a          810 c          819 g          801 t
ORIGIN      
        1 tcttaggaaa taaatgatac atgcgctttt cggactattg aaaactgccc ctgtgctaaa
       61 tttgtaaacc ctgcgccgac aggcttgcgt aaaactcttt tggtgataat gtgagccttc
      121 caaatccaga ctattccgat ttgggatcca ttttgccgtc ctggcgtgtg taaccgtttt
      181 atcaaggaat aagcagtatg cgtaaaatcg cattaattct tgcgatgttg ttgatcccgt
      241 gcgtttcgtt tgccggtttg ctgggcagca gtagctcgac cacgccagtg agcaaagagt
      301 ataagcagca gttgatggga tcccctgtct acatccagat cttcaaggaa gaacgtacgc
      361 tcgatctcta cgtcaaaatg ggcgagcaat atcagctgct cgacagttat aaaatctgta
      421 aatattccgg cggcttaggg ccaaaacagc gtcagggcga tttcaaaagc ccggaagggt
      481 tttatagcgt ccagcgtaat cagttaaaac cagacagccg ttactacaaa gcgattaata
      541 ttggtttccc caatgcctat gaccgtgcgc atggttatga agggaaatac ctgatgatcc
      601 acggcgattg tgtttccatc ggctgctacg caatgaccaa tcagggtatt gatgagatct
      661 tccagttcgt tactggtgcg ctggtgtttg gtcagccgag cgtgcaagtg agtatttacc
      721 cgttccgcat gaccgacgcc aatatgaagc gccataaata ttccaacttt aaggacttct
      781 gggagcaact gaagccgggt tacgactact ttgagcaaac ccgtaagcca ccgaccgttt
      841 ctgtggtcaa tggtcgttac gtggtcagca agccgttaag ccacgaagtt gtgcagccac
      901 aactggcatc aaactacacg ctccccgagg caaaataagc gccattcgcc tggcataatc
      961 ttttgccagg tttcattgcc cgtcagcggc tgtgtcgcaa tcaccgtgac cacatcattc
     1021 ggtgtggtct gcgagctgaa gtcgatttct acatcctgat ccagcagcgt tgccacgcca
     1081 aacggtgcgc ggcgggtgat ccagtgcaga ttcgtcgagc aatacgccat tacatagcgc
     1141 ccatccgaaa gcagcatgtt gaaaacgccc ttctgccgca gttcgtccgc cagtgaagcg
     1201 atatatttaa acactgctgc catgttgccc ggcgtgcgcg ggtaacgctg cgttaattta
     1261 tgcagcagcc agcagaaggc tttttcgctg tcggtttcgc ctaccgggcg gaagttgccg
     1321 gtttccagtg atttgtagcc cgtcagttgt ccgttatggg cataagtcca gttacgcccc
     1381 cataactcgc gggtaaatgg gtgagtattt tccaacgcca cctcgccccg attagcctgg
     1441 cgaatatgag ccaccaccga acaggatttt atcgggtagt cctggacaag tttggcgatg
     1501 ggggaattaa agctgggttg tggatcttta aatgtgcgac agcctttacc ttcgtaaaag
     1561 gtaatgcccc agccatcttt atgtggcccg gttccaccac cacgctgtac aagtccggtg
     1621 aaactaaagc agatatcggt agggacgttg gcgctcatcc cgagcaattc gcacatgggt
     1681 aacctccaca acagcgggag cgcatggccc ccggcgaaga ttgatttaag aattttcagg
     1741 tcggatgcgg cgtaaacgaa ttattcggcc tacggttcgc gtacagattt gtaggcatga
     1801 taagacgcgt cagcatcgca tcaggcatca gcgcacaagt gccggatgcg gcgtaaacgc
     1861 cttatccggc ctacgccaga cttacttaac catctctttt tcaattaact ggatcaggat
     1921 atggatcact ttaatgtgaa tctcctgaat gcggtcggca taaccaaagt gcggtacgcg
     1981 aatttcgata tccgccgtgc cagccatttt gccgccgtct ttaccggtca gggtgatcac
     2041 tttcatcccc ttctcacgcg ccgctgcgat cgctttgatc acgtttgcag agttaccgga
     2101 ggtggagatc cccagcagta catcgccttc gcgacctacc gcttcaacgt agcgggagaa
     2161 aatatcattg aaaccgaaat cattaccgac gcaggaaata tgactaacgt cagaaatagc
     2221 aatcgccggg tagcccggac ggttttcacg gtagcgaccg gtcaactctt cggcaaagtg
     2281 catagcgtcg caatgggaac cgccgttgcc gcaggaaagc actttgccac cggctttaaa
     2341 gctgtctgct aacaggaccg ccgcgcgctg aatggcgtga atattggcgt catcttttaa
     2401 aaagttagcc agcgtttccg ccgcttcgtt cagttcgtta cgaataagat cttggtacat
     2461 gaggatatcc ttcagcataa atgtaataga caaaatgcag tgtaccggat accgccaaaa
     2521 gcgagaagta cgggcaggtg ctatgaccag gactttttga cctgaagtgc ggataaaaac
     2581 agcaacaatg tgagctttgt tgtaattata ttgtaaatac attgctaaat gtatttacat
     2641 ccactacaac catatcatca caagtggtca gacctcctac aagtaagggg cttttcgtta
     2701 tgatgatttt gagtattctc gctacggttg tcctgctcgg cgcgttgttc tatcaccgcg
     2761 tgagcttatt tatcagcagt ctgattttgc tcgcctggac agccgccctc ggcgttgctg
     2821 gtctgtggtc ggcgtgggta ctggtgcctc tggccattat cctcgtgcca tttaactttg
     2881 cgcctatgcg taagtcgatg atttccgcgc cggtatttcg aggtttccgt aaggtgatgc
     2941 cgccgatgtc gcgcactgag aaagaagcga ttgatgcggg caccacctgg tgggagggcg
     3001 acttgttcca gggcaagccg gactggaaaa agctgcataa ctatccgcag ccgcgcctga
     3061 ccgccgaaga gcaagcgttt ctcgacggcc cggtagaaga agcctgccgg atggcgaatg
     3121 atttccagat tactcatgag ctggcggatc tgccgccgga gttgtgggcg taccttaaag
     3181 agcatcgctt cttc
//
LOCUS       DAJTSL010000262         3193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTSL010000262 DAJTSL010000000
VERSION     DAJTSL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.265"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            102..3005
                     /locus_tag="OO433_004035"
     rRNA            102..3005
                     /locus_tag="OO433_004035"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3101..>3193
                     /gene="rrf"
                     /locus_tag="OO433_004036"
     rRNA            3101..>3193
                     /gene="rrf"
                     /locus_tag="OO433_004036"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          850 a          700 c          986 g          657 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgaatcgta agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccat tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatattg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgctgatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaactaa ggctgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatatgtagg tgaagtccct cgcggatgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggtcagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgttgacccg taatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgactccttg agagtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaaggtg ttttggcgga tgagagaaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggcc gtagcgcggt
     3121 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     3181 ggggtctccc cat
//
LOCUS       DAJTSL010000263         3145 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTSL010000263 DAJTSL010000000
VERSION     DAJTSL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.266"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..142
                     /locus_tag="OO433_004037"
     CDS             <1..142
                     /locus_tag="OO433_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="activator of the allDC-ylbA operon involved in
                     allantoin utilization; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6052311.1"
                     /translation="MRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNP
                     RS"
     gene            211..1305
                     /gene="mnmH"
                     /locus_tag="OO433_004038"
     CDS             211..1305
                     /gene="mnmH"
                     /locus_tag="OO433_004038"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6052312.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            1421..1786
                     /locus_tag="OO433_004039"
                     /pseudo
     CDS             1421..1786
                     /locus_tag="OO433_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2348..2797)
                     /locus_tag="OO433_004040"
     CDS             complement(2348..2797)
                     /locus_tag="OO433_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052313.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFSHVAVDISIYTNVEDDYFFSYFSLTNDDGGRFKKTLTVR
                     AREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFLLVKLMLRIIL"
     gene            complement(2778..3145)
                     /locus_tag="OO433_004041"
                     /pseudo
     CDS             complement(2778..3145)
                     /locus_tag="OO433_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          841 a          727 c          713 g          864 t
ORIGIN      
        1 gatgcgccct ccttcgccat tgagtctggc atggcgcaaa tttggcagcg gcaaagcggt
       61 agaagatatt gtgaccttgt ttacccagcg caggccggaa atcagcggat ttttagaaat
      121 tttcggcaac ccacgcagtt aaaaatcaag tttccttgcg cgttggcgcg taaaatagcg
      181 cgcaagctga tttgaggttc acttttaact atgcaagaga gacacacgga acaggactat
      241 cgtgccctgc tgattgctga tacgcccatt attgatgttc gcgcccctat cgagtttgag
      301 caaggcgcaa tgcccgccgc tatcaatctg ccgttaatga ataacgatga acgcgccgcc
      361 gttggcacct gctataaaca gcaagggtca gacgcggcgc tggcgctggg gcataaactg
      421 gtggcgggtg aaattcgtca gcagcgcata gacgcctggc gggcagcgtg cctgcaaaat
      481 ccgcaaggta ttctctgctg tgcccgtggc ggtcaacgct cacatattgt gcaaagctgg
      541 ttgcatgcag cggggattga ttatccgctg gtggaaggcg gttataaggc actgcgccag
      601 accgcgattc aggcgactat tgaactggca caaaaaccga tagtgctgat tggcggttgt
      661 accggcagcg gtaaaacgct gttagtgcag caacagccga acggtgtcga tctggaaggg
      721 ctggcgcgtc atcgcggttc ggcgtttggt cgcacgttac aaccacaact tagccaggcg
      781 agttttgaaa atctgctggc tgccgaaatg ctaaaaaccg acgcccgtca ggatttgcgc
      841 ctgtgggtgc tggaagatga aagccggatg atcggttcga atcacctgcc ggaatgtctg
      901 cgcgagcgaa tgactcaggc ggcgattgcg gtggtagaag atccgtttga gatccgtctt
      961 gaacgcctga acgaagagta tttcttgcgt atgcatcatg attttaccca cgcgtacggc
     1021 gacgaacagg gctggcagga atattgcgaa tacctgcatc acggactttc ggctattaag
     1081 cgtcggctgg ggttacagcg ctataacgaa ctcgctgccc agctggatac ggcgctgaca
     1141 acgcaactca ccaccggcag caccgacggt catctggcct ggctggtgcc gttacttaaa
     1201 gaatattacg acccaatgta tcgctatcag cttgagaaaa aagcggaaaa agttgtcttt
     1261 cgcggtgagt gggcggaagt ggcggaatgg gttaaggcgc ggtaatgagg gacgtatttg
     1321 tattagtcgc gacttgatct gacacatggc cttgaaaggt tgagagttac cggttttgat
     1381 atgagtgtcg aatccttatc caaaacaaga ggtaactctc atgcttcata ctgccaatcc
     1441 cgtcatcaaa cacaaagccg gtttgctcaa tctggctgaa gaactcagca acgtatcaaa
     1501 agcctgtaaa atcatgggcg tttcgcgtga tacgttttat cgttaccgcg aactggtcgc
     1561 tgaaggcggt gtggatgcgc agattaatcg tagtcgccgc gctcctaacc ttaagaaccg
     1621 taccgatgag gcaactgaac aggctgttgt tgattacgcc gtcgctttcc cgacacatgg
     1681 tcagcaccgg gccagcaacg agctgcgtaa acaggggggt tttatctccg acagtggtgt
     1741 ccgttctgtc tggttactcc ataaccttga gaatctcaaa agacgatact gaacaaattt
     1801 tatcaggtga cgttccgtaa aaagttgtat ggagttctcg atgcagtcgg cgcgactatc
     1861 agtacccccg ctgaaccctg gactatcggg ccgccgtact ggggcatgtc accctggcgt
     1921 gccgctggtt ttccgctaat ggtttatttc ctctttcttt cccggcaaga atttatttta
     1981 atccggaagg attatttatt ttcagatagg gactcacatt aaacacgtaa cattttaatt
     2041 aatgcatggc tgaatatgta ttacacaatg tcagatatcg taaaagacca gtgcaattct
     2101 atgtaaactc tctgactgtt cattttattt gttgtttcag ggtcggaact caccctcaat
     2161 aaacaactga atacgagtag atattcatcg tctgagctat atggctttac acaatagccg
     2221 acatgtcttt tctcagtcag gaattttact tttcctcact acgaagttga cgacttcgat
     2281 atgggataga ctcttaattc aagcaattac tctgcacaag catcaggcaa tccagctcca
     2341 atatttgtta taaaataatg cgtaacatta atttcaccaa caaaaaaagg tttacaccat
     2401 ccccttgccc tggtatctta ccatatttac accacacttt ttttgtatct ggatcattct
     2461 ttgatacgat tttcgccact tgttccctgg ccctcactgt caatgttttc ttaaacctac
     2521 ccccatcatc atttgttagg gaaaaataag aaaaaaaata atcatcttct acatttgtgt
     2581 atattgatat atccaccgct acgtgcgaaa aaaaatccgg gagccatgta aaaactccat
     2641 cattttctgg tattgccata tagtttatgt aatgtttact tacaagagat tcatctttgt
     2701 ataaataaag ataagtaatt acgcataaaa caacaatgat tataatagca aaaataaata
     2761 ttatcatctt tgatagatta cttgagatag ccagcatctt gtaaagcctt tatcgttttt
     2821 ttatgctctg gattaatata atcactacat ctatctgagc aatctgttgt tgatggacat
     2881 gtcaacccat ggtcatttac agccaagtct tttttattat cttcaatcat ttcagaatgg
     2941 gatagcttta cttttctgcc gtacataccg aacagattta atccgtaatc tctaatctct
     3001 ttttcataac ccagcccttt cgccgatcgg ctaacaccag cgtcatttaa tttagacact
     3061 cgacaaaatg ccatacaatg gaaaaattga tcagtcccac ctaaatttaa tctcttcata
     3121 agtatcagat aatatatccc atgct
//
LOCUS       DAJTSL010000264         3038 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTSL010000264 DAJTSL010000000
VERSION     DAJTSL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.267"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            211..370
                     /locus_tag="OO433_004042"
                     /pseudo
     CDS             211..370
                     /locus_tag="OO433_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(588..2678)
                     /gene="iha"
                     /locus_tag="OO433_004043"
     CDS             complement(588..2678)
                     /gene="iha"
                     /locus_tag="OO433_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HCR6052314.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT          760 a          806 c          693 g          779 t
ORIGIN      
        1 aacttaattg tgacaaaatg gcgattgaga aggtgcaatt gacttatccg gattcggtcg
       61 tggcgaggtg gtgcccctgc attctgctaa ccgggttatg agccaggagt cagcgataga
      121 caaagggact tatctgaccg ggcccccgga aaaatggcgg gagagaatga taccttcctg
      181 ctgacgagac cggactttga gatgacgagc gatagtgcgg cagtgatgta cagcctgatc
      241 ggtagctgca aaacttaacg gaatcgagcc ggaaacgtgg ctacgccacg tgatcagtgt
      301 aatcaacacc tggcctgcca accgcatgaa agagttgttg ccctggaacg tcactctctc
      361 tgtaaactaa tttttacccc acgtccttca cggtgcgctt acactctgga caagtatgta
      421 aatgataagt taaaaataat ccgtgcattt cctgtgacga tattagttgt tggtaacgaa
      481 atctcattag cggatcggaa atgcacttaa cctaatacgt tgccactgtt ccgccaggag
      541 ttgcagtggc aacgtattct accgtcagtg atagcgtttt gttattatca gaactgatag
      601 ttcagcgaca tccagtaatt tcgctcaggt atcacatatc ctgttgttga tgatcccgtc
      661 tggaagtaat caccggcata cagcgtactc ttaccggcac tgtacaggct cacgtcactg
      721 taatccttgt tgagcaggtt attcaccgca gcattcagcg tcagggcatc cgtcatcttc
      781 caggacagac ctgcatccac caccgtccag gctttcaggt attctccttt ctcatcatac
      841 actttcttct gtacagcgct cagtgacgaa taattctggg tgaaacgtgg tgttttcccg
      901 cgataacggg cacccagcca tgatgccacc tcttcggtga tctgccagtt cagtttcgca
      961 ttcaccatgt gttcaggggt ataactcagc ggcgcacctt tgttatcacc atcacgttgt
     1021 tcacttcggg tccaggtgta attcagtgac agcgtgacat cctctgacca cagcggcaat
     1081 gtgccggcaa attccacacc gtgcaaccgg gcctttccgc tgtttacata gctattggtg
     1141 ttatcattta tggaataaga gacaatcttg ttggagaagt cagtcataaa acctgtgaca
     1201 ttggcattca gaccggcggg gttatcgtaa tacaccccag cctcataact gacgctctct
     1261 tccggcttca ggtcggggtt accaagtaga tttgtttttc cctgcccgga cacaccacta
     1321 atccctttat gtagctgccc cattctgggt gccttatatc ccgtggtcac accgcctttc
     1381 agcgtccagg catctgccac atcccagacc agatatgcac gcggactgaa gtgtcccccg
     1441 aattgctcat gatgttcata gcggctgccc gcagtcagcg caagtgcatc cgtgagatgc
     1501 cactcatcct cagcaaatac cgaccagctt ttctgccgga aagtttcacc tgtgctggca
     1561 aggacaactc cgtctttcat ggacgagctc tgaaactcgc cccccaccgt aaccagatga
     1621 gattctccca gaggggtaag cagtaatgaa ttcaggataa ggttcgattc cttaagctcc
     1681 cgcggctgac cggcaagccc ccatttgtcg cgcttcagta cactgcgtac gagctcacga
     1741 cctttatttt ctgtctcgtt ccagttcaga tacgatttcc atgttccgaa ggtgaaagta
     1801 tgatcatagc cagctgaaat tttgttccgc tcatagcgca gggtccggtc atatcccccc
     1861 gtcagactcc ccagttgccc atcccggtta tcataacgct gccgggtggt atccatatca
     1921 aaccagagca catcctgctc cgacgccttc cagtcaagac gtgcaccaag attataattc
     1981 tgtgactccg tgggataagg aatacgcgtg gctgctgtat cgctcagtga tgtgaccgat
     2041 gagccctgac gctgttgtgt gctaccgcgt acctgcaggc tgacagaatc atccacaagg
     2101 gggccactgc tccagaaatt aaactggctg ctgttacccc atttgttgct ttcctgcaga
     2161 ttcagccctg cattgacgga agagagccat ttgtctgcat tctttctggt aatgatattc
     2221 accacaccgc ccatcgcatc agagccatac agtgtggaca tcggccccct gataacctca
     2281 atacgctcaa tggcggccag agggggcatg aacccggtat tcatggcaga aaaaccgttg
     2341 ggagtcacgt cactgcttcc gccctgacga acaccatcaa tcagtatcag cgtgtaactg
     2401 gctggcattc ctcggatgct gatttccagc ccaccggttt tacccgtacc actttcaaca
     2461 tccacaccct ctactgatct cagagcctcc gccagatcgt ggtactggct ggactgcaat
     2521 tcctcctggc taatcacaga aacactggcg gctgcgttag tcagcttttt ctcatagccg
     2581 gatgccgaga caatcatcac atcctctgca gcagctatgc tgctggctga aaatccgaga
     2641 cagggaatga ctacggaagc cagagtggtt attcgcatat tactaatctc cgatcatgtt
     2701 aaccggcaag acaatacaca aatacaaaca ataatgagaa taatttatat ttgcctttta
     2761 caaaaaataa agattattaa caaaactaaa tataaataac aactgcctcg gcatgccagt
     2821 taacagctat ccgtatggcg tgttcggttt tgcgaataaa aaatggttac tgtagaagcg
     2881 gatttccacc acatgtcaac catgaatggt cagtggtgcg agacgactgg gatgatttgg
     2941 tcgatgactt cttccggtga atactgatag aaacattgaa atattgtaag agaaagcgtt
     3001 caaaattctg tggcacgtga aaaattctac agcgtcat
//
LOCUS       DAJTSL010000265         2993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTSL010000265 DAJTSL010000000
VERSION     DAJTSL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.268"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..776)
                     /locus_tag="OO433_004044"
     CDS             complement(<1..776)
                     /locus_tag="OO433_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6052315.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSKNYGCSLDNTDVNWCSVVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETIINPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GYLGIMTEGENFLCRLLTIEDEVLNVLLRENFNEQLDTNI"
     gene            complement(1038..2628)
                     /locus_tag="OO433_004045"
                     /pseudo
     CDS             complement(1038..2628)
                     /locus_tag="OO433_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
BASE COUNT          964 a          547 c          470 g         1012 t
ORIGIN      
        1 ttaatattag tgtcaagttg ctcattaaag ttttccctta gcaatacgtt gagaacttca
       61 tcttctatag taagcaaccg gcaaaggaaa ttttctcctt cagtcatgat tcccaaatat
      121 cccgtagcaa aaagcgcgta caaaactgca ccaactgaac catcggtatc cccaaaatag
      181 gtcggcctgg tacttgtggg atcaacaggt gaacaattgg agaaatagat tctgtcagcg
      241 aactgggggt ttataattgt ttcgaattta atatcaaata cattgtattg cgatattttc
      301 cagttccaga tgggtttgta cgactcagta actgcaacat cattagcatt atcatttact
      361 gtcatattat taagtaaata aacaacactg caccaattaa catcagtatt atcaagagag
      421 caaccgtaat ttttactatt aattgtcaca ataaactgat aattattatc cctacgtaga
      481 atatgagcac gcatgcccga tagcatacta tatttttctg aatgatccgt ttgtatggta
      541 aatgaaattt tgtcatcttg aatagattgt tggaaccgtt ctatttgcca atcatctatt
      601 ttttccacaa cctcaccgag cattgtcctg gcaatacttg atatatcatc acctatacca
      661 tttgcaatat acccaaacag ttctttagtg aagttggtaa catgctcttg caccatatca
      721 tttgttgcga ttgtcgttgt actgtcgtta gtgcctgcca cagttccaat tttcatcttt
      781 tacctcatat tattcaccgc gaggaataca tagttaacat tttatcttat gacatacacc
      841 tcatgttcga tggaaaaata attatatttt gcacaaatca ttcagcctta aagcataata
      901 tgaaatttct tatttcatag cagagaaagt ccctaaagat tgtagaggcg tcatcagaac
      961 atgtctctac aatcgattaa gaaaacctat gaaatactgg caggattaaa aagatatacc
     1021 acagcactca tggagtttta atgtggttga aaacaactaa gccctgggac acctttttta
     1081 tcaccacgtt cgttaggagc tatcttccag gaatttagcc ccatggtata attatcaatt
     1141 ttttgatgct gatctacccc taatccataa tggccatatt tctgtttagt ttttttgagc
     1201 gtctgtccac atttgaagat atcttcaggt tttggtctga aagcagtagt gttattaaaa
     1261 taattgtcat atacaggtgt ggatgctata gcattgcttc taattagatg gataaaacga
     1321 tcatccatgt gttgtttatg ttttttatta aatatatttg ctaattcctc aggatcaatt
     1381 gggtctttca tctgattaaa tggaacctgc aaagcaccta gacaataaca ttcattttct
     1441 tcatttctta ttattccgtg atgactgctt aaaactttcg tttctgagtc ataattacaa
     1501 acatcgagcg ttttaattaa gttatacgta tcaccggcag agagtttatg atttgctagt
     1561 cgtgcagtat agttaccatt gaaattgatt tcataatttc cagccagaac gacgtcattt
     1621 ttatttatac gactatcttt attactctcc atattccgca tagaatttag caaagtaagg
     1681 atatatttat caccaaaaat agtactgaat ctatcgcctg tatgatcccc aagaaagata
     1741 caaggggtaa tcgcatcgcc ttttaaaaaa acatgttgat gcaacttatt caagatcatg
     1801 tctctcgtat ctaataaagt aaagagtttt tccaattttt tattttcata tgcgaatgaa
     1861 gccatgtctt cgtgttttaa taattcacat aataaactcc accccttttc cctgatacct
     1921 atatgtcctg aaacgagcaa tgcataaagt acagcgccaa ctcttccatc tgtatcgcca
     1981 aaataagccg tgttgggatt ttgtgggtta atcacaccac actgtttata aaaatctatt
     2041 tttgaaaaca cagaattaac atagctagca aacttatcct taggaaacgt ctctttagag
     2101 acatcccaac gctagtcagc tttaaaatta ccgctaagaa gagtaaagaa attttcaggt
     2161 atgatagaaa ggccattaac tttataaatt atgtctgatt tatctccgga gaaactaata
     2221 aaataataac catccttaca ctcaatcgta acggtaatgg gagacgacaa ttttattaca
     2281 tggttattgt ctggtataat tgtaaagata aacgtgtcag tagtattatc ggcgaaacta
     2341 ttatattctt gaatatctat agatggctca ttattgtaaa accatatttt tttctatctg
     2401 agaactatat tcatttacct tccagcgacc atgttgcagg aaatagatga tatgcaggcc
     2461 taaatcgtta tctatttgcg aatgtttatc cagcttatca atttcaacta tctttgaacg
     2521 atgtttttta tcaaccttgc catggtgttg atgattgtta acttgatctg aatgcttatc
     2581 ttgatggctt gaagagatgt gcttaaatat attgtgaaat actttcataa ataacccgca
     2641 atatttataa cttgttagta acaaaataaa cgatacatgt ctgaaagaca tatttacgac
     2701 gatacaaagt aataatactt atatatccga tgattttaag caccgagact atccggtctt
     2761 ttactaaata atggaaaaca atccatccct ggaagataat tgaaaaaaac aacagaataa
     2821 gaattaatct taggataaac atttatttat catggccttt tgaacgctga aaagtagcgc
     2881 tctgtaaagt aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtca
     2941 ttgtattaac aggctgaaat tatcattaat gcacatttca atattagcag gga
//
LOCUS       DAJTSL010000266         2943 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTSL010000266 DAJTSL010000000
VERSION     DAJTSL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.269"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            578..1294
                     /locus_tag="OO433_004046"
     CDS             578..1294
                     /locus_tag="OO433_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052316.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            1846..2250
                     /locus_tag="OO433_004047"
     CDS             1846..2250
                     /locus_tag="OO433_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052317.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2313..2943
                     /locus_tag="OO433_004048"
                     /pseudo
     CDS             2313..2943
                     /locus_tag="OO433_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          825 a          605 c          667 g          846 t
ORIGIN      
        1 attgtgggcc ataaatattc caaaaaaata gcccgctccg tttattaccg gaacgggcga
       61 gagcatgaca ttgtctgtaa ttataatgag actgctgatt ctacttattt ttaatttcaa
      121 gaaaagcgtt ttctacaaag aattatgaga tttaatgttt tgtttagaca atattgtcat
      181 tctgttcaat aatgaaagtc ttcatcaaca acaagtaaaa acaacgtccc tctcatcgaa
      241 ccggacttgc agatttcccg catccggctc tcttgcaaca catcagacct tcacgttcgg
      301 gcggttatgg ggaaggtcca gcaagctgta tgagccctaa ctgtttgaat accagtagat
      361 taccagaaac ttctatcgat tgaagacatt accaactagc gttactaaat gttgaccaag
      421 ttttgttcac ttgtctggtt ttgatagtag cattctgcag agtattttga tatgataaat
      481 atgcattaac aatgatatta ttgttaattt cctagcatga aaattaaaag atcaactttt
      541 tatctcgaat attttttaca ttatcagttg gtttcttatg aatgacaatt cattgcttag
      601 aaattcttca ttattcatcg cctatatggg atgtgtaggg tgggtcagtg cttattctta
      661 tggctgggga acctcttttt actatggatt tccatggtgg gttgttggtg caggtttgga
      721 tgatgttgcc agaagtttac tctatgctat catagtaatg ggtatacttt ttaccggttg
      781 ggggattggt attttatttt ttcttcttat aaagaaaaga agtaaaatac aagacttgag
      841 tttttttaga ctattttttg ctattaccct actgtttttc cctgtaatat tcgaactttt
      901 aattctcaaa caatatttca tacttccatt atcactttca tgcattatta gttcattggt
      961 tatttcaata ataattcgca tatatggacg gatcttttct gtatcatgtt tttcagatat
     1021 cccttttgtt agagaacacc gcattaagtt aattatggct ggttttttgg tttatttttg
     1081 gcttttttcc tttcttgttg gctggtataa acctcaatta aaaaaagaat atcagatgct
     1141 ttgttataat aatagttggt attatgttct tgctcgttat gattcacggc tcgttttgtc
     1201 tagttcattt aaagatgaca gtaacagatt cttaatattt aatacagaac agtcgggatt
     1261 ttatgaaatc aatgatgttt atgtcagaaa gtaataatcc tctcccacaa agtgaattaa
     1321 acgaatatat aatgcgttcg aagtatatgt attgagctgt catactgaga aacaaacctt
     1381 agggcagcac aattaaagcc agaaacaact atgcaagaaa gttactaaat aagtgctcgg
     1441 aacagtaaaa cactcagggg ggactcagtc cagattatgt aatcagataa attgccaaac
     1501 atcacaaatc accagcagga ctgagtgatg ccgagcattg caccaatacc ccgtgatgaa
     1561 cgatgcctga tgcagaaagc tccctataaa acaaagaaca aaaactacga ccggcgattc
     1621 accgtcatac tgatgctgca tcgtggtgac actgtctgtc actttcatgt tgactgaaca
     1681 actctgctgc gaagaaggtg taccgccggc gggatttgat tgtccggcgt ctagcttcca
     1741 cttccctgat ggaactgaaa gttgttatgg ccggtcagct tgccctgtcc gcaggacatg
     1801 aggtgctgtg acggacagca ccagtaacgg aaagtgacaa taaaaatgaa tactgcgctg
     1861 atagttgcac taatgtgtat gtggtatgcg gtacccgctg ctgcaaaaga gactttactg
     1921 gctatgccac gtaattcaac agaacattgc tatgcggaga ttaatgtgca cggaccatat
     1981 ggggtgtatt tccgagtggt cccgcatccc cccgggggaa agagctgggt cgaatgtaac
     2041 agtgactact attactctga taaaccaccg ggagtacaga tactcggaac ccgtgcaggg
     2101 tgtcgggtat acggaatctg cgggaccacc tcgacactgc atgtggctgg aagaggggtt
     2161 gtgtgtatta aaaatatatg cagtccacgt gggatgataa ttcacaggat acggaaacgt
     2221 cctgtagtgg ctgtaagcga tgagatgtga tcttatccag caatagtgga cacgcggcta
     2281 agtgagtaaa ctctcagtca gaggtgactc acatgacaaa aacagtatca accagcaaaa
     2341 aaacacgtaa acagcattcg cctgaatttt gcagtgaagc cctgaagctt gctgaacgca
     2401 tcggtgttgc tgccgcagcc cgtgaactca gcctgtatga atctcagctc tatgcgtggc
     2461 gcagcaaact gcagcaacag atgacctcgt ctgagcgtga aagtgaactg gccgctgaaa
     2521 atgcccgcct gaagcggcaa ctggcggtgc aggcagagga actggctatc ctccaaaagg
     2581 ccgcgacata ctttgcgaag cgcctgaaat gaagtatgtc tttattgaaa aacatcaggc
     2641 tgagttcagc atcaaggcaa tgtgccgcgc gctccgggtg gcccgcagcg gctggtatac
     2701 gtggtgtcag cggcggacag ggataagccc tcgtcagcag ttccgtcagc actgcgacag
     2761 cgttgtcctc gcggcggcct ttacccggtc aaaacagcgt tacggtgcac cacgcctgac
     2821 ggatgaactg cgtgctcagg gttaccactt taacgtaaaa accgtggcgg caagcctgcg
     2881 ccgtcagggg ctgagggcaa aggcctcccg gaagttcagc ccggtcagtt accgcgcaca
     2941 tgg
//
LOCUS       DAJTSL010000267         2856 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTSL010000267 DAJTSL010000000
VERSION     DAJTSL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.270"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            233..544
                     /locus_tag="OO433_004049"
     CDS             233..544
                     /locus_tag="OO433_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6052318.1"
                     /translation="MFNVITHPAALDELLELPDNLRGRMTRLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     N"
     gene            544..831
                     /locus_tag="OO433_004050"
     CDS             544..831
                     /locus_tag="OO433_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6052319.1"
                     /translation="MKVKGVPFSQVKQKLLSTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAHACGATIDIHAVY"
     gene            complement(1368..2555)
                     /locus_tag="OO433_004051"
     CDS             complement(1368..2555)
                     /locus_tag="OO433_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6052320.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPRIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPVSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(2555..>2856)
                     /locus_tag="OO433_004052"
     CDS             complement(2555..>2856)
                     /locus_tag="OO433_004052"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052321.1"
                     /translation="VVKLHEDDFFDEEDAEVLRFDSFDDAVECCADLNIPFFVDAGNK
                     KLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVHWPDGVAPPF"
BASE COUNT          739 a          770 c          665 g          682 t
ORIGIN      
        1 taaggtgaaa tctgtcaatc agtaccggct tacgccgggc ttcggcggtt ttactcctgt
       61 gccatatgca acaacggagt gccgccttcc atgccgctaa cgcggcatca aaaaatccct
      121 cttctccagt tgcccccctc cgaactcaga gtgtattttc atttattgat gaaatttact
      181 gtttgcgcaa ttttcctgtt ttagtgtatt ttgtcattct ggaggctata atatgtttaa
      241 tgtgattact cacccggccg cactggatga acttctagaa ttaccagaca atctgcgtgg
      301 tcgtatgacc agactgattg aacggctgga acaggaaggt aacaaaatga aaatgcctca
      361 cagccgtgta attggcggag ggctttttga actgagagtc ggggataaaa atatcgcaag
      421 gacactgtac gcttatgcaa cgggtaacga aatctatctg ctgcatgcat ttgtgaaaaa
      481 aacgcaaaag acaccaacga aagctattga aattgccagg atgcgtctga aggagatgaa
      541 ctgatgaaag taaaaggtgt tccattcagc caggtaaagc aaaaactact cagtactcca
      601 gaagctgtca aagggtatga ggatgctgac aaagaactgg agatggtgga aatgttatac
      661 gaaatgagag aaaaagcggg attaacaaag tcagcactgg ccgaacgaat ggggttacgc
      721 ccgtcagcca tcagtcgcct ggaaagtaac cctttagggg ccagcatgaa aactttgaca
      781 aaatatgctc atgcctgtgg cgcaaccatc gatattcacg ctgtctattg aggggtccgc
      841 ttggaattcg gaaaataaac cacgttaaga ctatttttta gataaatgac tttcaggaat
      901 cgccaagatg gcgggataac agcggttttg gtgtcctaaa tcgtacaatt ctgtacatcc
      961 acgcaaggga atgtacggaa gtaagccaga cggttatcgg acggtcagaa catttcgtac
     1021 agcctttacg ttcagtgagt tagctggcct ggacgtaacc actcgttagc tcaattctct
     1081 tagcgtggga aaatttccga attccaagcg gaccccttta tccttgcccc gctgaatgag
     1141 gaaacgctgt tccgggggat tatgctgaac gtcttccgtt catcgatagg aattaaaacc
     1201 ccaaaaagat taaaaaacac cacaaaacgg atgtttcttc aacaccactt ttgctcatat
     1261 gaacggaacc gacgattaaa ctggatggct ctgattgatt cagggtatga atggcggttt
     1321 tttgctccgt ttccctcaaa atggacgcta cttaccctct gcggctctca gccgcaccac
     1381 cgcatccggg ccagcggctc atgcatcagg acctgctctg ccagacggta gccccgcttc
     1441 agccccgtaa aacgcatctg actcccgcac agcacgcact tcagcgggtc aaccttcagt
     1501 aacctctgat acatccctct ccaggtgatt tgcatcgccg tttttctcac tgtctccgtt
     1561 atgatgtaca ccacttcttc cagtaaccgc cgtttcgccg gactcagaaa accgtagtac
     1621 ctcaccatac ggaacccctt atccgccaca tgccaggaga acctttccat gaactcatct
     1681 ccactcatca acaggtattc ttcccgtttt gttcggtgac tgttgtaacg cagtccgatt
     1741 tcatcctgac cggcataatg ctccagacga ctcaccggca ccggtggctt tttcaggtaa
     1801 gagccaaagt acaccgccac atgggtggca ttatccatca cccgggatac gttgacattc
     1861 cagccacggc ggtaatgcgt gtccaggaag cgattccatt cccgtttact gcttccttct
     1921 gctgccagcg catccggcat caccaggtca gggtatttcc gtgacagtaa ccgtgttatc
     1981 cggtagcgcc acatgctcat caccttacgg gcgtaaaaat gaagattttt ccaggtgtgg
     2041 cccgacgtca caccaccggc agttgtcgat aaatggatat gcggatgcca ctgctggtca
     2101 cgcccccatg tgtggatcac cgtgaatatc ctcggctcca catctgcctg atggcagatt
     2161 tccagtatca catccgctgc aatgcggctc atctctgcca gcaaccaccg gttgtggaac
     2221 accagggacc agtactggca gggtaacgtg aacacaatat gctgccatgg gcagtcgggg
     2281 accaggctca gcagatactg tatccactgt gcgccagcct tcaccccgca gtgcgggcag
     2341 gagcggcttt tacaccggaa gcagaccttt tttgtatggc aacagtccgg tgatgaacag
     2401 caccactgtg tataccccat cagtgtggtc ccgcacgcca tgattttggt caccgactca
     2461 atcaccaccg gacgtactgc cccttccggc tgcttctcca gccagttaag ccagcggttt
     2521 ccctgctgaa agatatcggc aaaacgggga agcatcagaa gggcggggcg actccgtccg
     2581 gccagtgaac cgtgccacac tccgggcagt acataccgcc ggcgctgata ccggaaagaa
     2641 tggtcgcaaa ttcccgctcc gtgcagcggg caatttccgg atacccttcg tcatcaacac
     2701 gtacaaacca gaagaccagt tttttgtttc cggcatccac aaagaacgga atattcaggt
     2761 ctgcgcaaca ctcaacggca tcatcaaaac tatcaaagcg cagaacttct gcgtcttctt
     2821 cgtcaaaaaa atcatcttcg tgaagcttca cgacat
//
LOCUS       DAJTSL010000268         2842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTSL010000268 DAJTSL010000000
VERSION     DAJTSL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.271"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..136
                     /locus_tag="OO433_004053"
                     /pseudo
     CDS             1..136
                     /locus_tag="OO433_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            386..1252
                     /gene="ospB"
                     /locus_tag="OO433_004054"
     CDS             386..1252
                     /gene="ospB"
                     /locus_tag="OO433_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6052322.1"
                     /translation="MNLDGVRPYCRTVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            1582..2322
                     /gene="phoN2"
                     /locus_tag="OO433_004055"
     CDS             1582..2322
                     /gene="phoN2"
                     /locus_tag="OO433_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6052323.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(2675..2842)
                     /locus_tag="OO433_004056"
                     /pseudo
     CDS             complement(2675..2842)
                     /locus_tag="OO433_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
BASE COUNT          931 a          517 c          527 g          867 t
ORIGIN      
        1 agcggtaaga gaagttcatc atacttggcg cagttacgcg atgtttctgc tagaggtgat
       61 gccgtaaaga acggcagaac attaccggaa caagatagcg gtctacctgc actggtatca
      121 gacccggggc ttccccggat gatctccacg gtactttagg tactgtgtct atattatgta
      181 ggtaggatca attaactttg tagttgaaat tctttcattg aaataatgaa taaaaaagac
      241 aaaccgcatt aaacaatgcc agaatataat tgcatttttg ttccgttttt ttaaatcata
      301 gtatacaaag aagcttgcat actatatgaa aatataacac cctttactat gatgtttata
      361 aaacaatata tggagtcatg taggtatgaa tttagatggt gttagaccat actgtagaac
      421 agtcaataaa aagaatgaaa gcatatcaga tattgcattt gcacatataa taaaaagggt
      481 aaaaaattca tcatgtactc acccaaaagc agcattggtt tttttaggag agaaaggttt
      541 ttgtgatagc aatgatgttc tatctattat gggacaacaa ataccaagag tatttaagaa
      601 caagatgtta tatgattatg tttttaaaaa tgaaaaaagt aaaaatgatt ttctaaaaat
      661 ggctgaatca tggctaccac agagtgaacc aatagtaata aataatgatg atgacgcatt
      721 gaatgctgct gcttattttt ctgtaaaaaa agcgaaaata aaaacagtaa acgatactga
      781 ttttaaagag tataataagg tttatattct tgggcacggt agtcctggtt ctcatcaatt
      841 aggccttggt tcggaactta ttgatgtaca aacaatcatt tcaagaatga aagactgtgg
      901 tattctaaat gtgaaagata tccgttttac ttcatgcggc tccgctgata aagtggctcc
      961 taaaaatttt aacaatgccc ctgctgaaag tctttcttgt atccttaact ctctgccttt
     1021 ttttaaggaa aaagaatctt tgctagagca gataaaaaaa caccttgaaa acgatgagtc
     1081 attgagtgat ggtctaaaaa tatccggcta tcatggatat ggagttcact atggtcaaga
     1141 gctttttccc tactcacatt atcgttcaac ttcaattcct gctgatccgg agcatacagt
     1201 aaaaagaagc tctcagaaaa agacttttat tattaataaa gaactggatt agtataaaat
     1261 ttttaaccta tagttaattg tttgcattta agatgaagtt atttttttct ttagtagaaa
     1321 agtgcttccc tatgagggaa aatcacaaat catcataata cagagacaaa acgatacgaa
     1381 caaatagata aaaaacatca ttcttttact acattatttt tcgtgaatat gaaaaataat
     1441 gttattactt taatataaga ctattttttg tttttccatc actctgttca aatttttccg
     1501 catgacttgt gttttttgta acacagctcg ttttttacag ctgaccaaaa tcatcaatta
     1561 attatgctaa ggaaataaat tatgaaaacc aaaaactttc ttcttttttg tattgctaca
     1621 aatatgattt ttatcccctc agcaaatgct ctgaaggcag aaggttttct cactcaacaa
     1681 acttcaccag acagtttgtc aatacttccg ccgcctccgg cagaggattc agtagtattt
     1741 caggctgaca aagctcatta tgaattcggc cgctcgctcc gggatgctaa tcgtgtacgt
     1801 ctcgctagcg aagatgcata ctacgagaat tttggtcttg cattttcaga tgcttatggc
     1861 atggatattt caagggaaaa taccccaatc ttatatcagt tgttaacaca agtactacag
     1921 gatagccatg attacgccgt gcgtaacgcc aaagaatatt ataaaagagt tcgtccattc
     1981 gttatttata aagacgcaac ctgtacacct gataaagatg agaacatggc tatcactggc
     2041 tcttatccct ctggtcatgc atcctttggt tgggcagtag cactgatact tgcggagatt
     2101 aatcctcaac gtaaagcgga aatacttcga cgtggatatg agtttggaga aagtcgggtc
     2161 atctgcggtg cgcattggca aagcgatgta gaggctgggc gtttaatggg agcatcggtt
     2221 gttgcagtac ttcataatac acctgaattt accaaaagcc ttagcgaagc caaaaaagag
     2281 tttgaagaat taaatactcc taccaatgaa ctgaccccat aaagctggac agcctgtatc
     2341 aggctatgga gggcccatag acaaatctac cctatatgag cataggagga gtctatgggc
     2401 acaccacgtt ttacccctga atttaaggga ttactggaaa ggctgggaca tatccctccg
     2461 gcagaagcag aaaaagctta ttatgctgcc atcggaaacg atgatctggc aacctgagtt
     2521 cacagataaa acattctcta ggaaactcgg ggcggttccg ttcaccacat gcaatgtggt
     2581 gttgcagggg aacggtctgc accatcccct atgtcgatca atataacaga aatgacaact
     2641 tcagattcag ggcacaacct aaatatattt taggtcacct ctcaaatcgt ttgcctgata
     2701 ccgctccatt ttttaataaa aaaatcaatc atttttgaat ctttgctttt cattgcattg
     2761 tcaagcatag tgtccccact atttgctttt tgaaattttt tgtttacatc aaccaatcca
     2821 ttattgataa aatattctag ca
//
LOCUS       DAJTSL010000269         2828 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTSL010000269 DAJTSL010000000
VERSION     DAJTSL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.272"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(49..233)
                     /locus_tag="OO433_004057"
                     /pseudo
     CDS             complement(49..233)
                     /locus_tag="OO433_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(395..469)
                     /locus_tag="OO433_004058"
     tRNA            complement(395..469)
                     /locus_tag="OO433_004058"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Arg,seq:cct)
     gene            complement(545..1477)
                     /locus_tag="OO433_004059"
     CDS             complement(545..1477)
                     /locus_tag="OO433_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6052324.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETHDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1771..2526
                     /gene="mlaA"
                     /locus_tag="OO433_004060"
     CDS             1771..2526
                     /gene="mlaA"
                     /locus_tag="OO433_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6052325.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          704 a          658 c          674 g          792 t
ORIGIN      
        1 catcttttac ggggtgaagg tagatttcgt atgaagggta tctgttgtct cctgcaaagt
       61 cataagcgga tattttccta cggtcagtat tttttcctta ccgtcaatct tgtagcgaag
      121 ttgccatacc ttttcccgga tacaggaaca taaaggtaca ggccattacc atcgagaagg
      181 cggtatggtt tttctttcgg ctttgctgct tcaatctgct taacggtgag catgggtaaa
      241 aatccggtgg gtaaaattat tttatccact ttttacccgt catggagtgc ggctgtcaac
      301 gatctgacgc gaaccatgac gaaccgtgaa tatacggaag gcttgatatt caggggattt
      361 tgcggactgg tacggatggg agcgaactga taaatggtgt cccctgcagg aatcgaacct
      421 gcaattagcc cttaggaggg gctcgttata tccatttaac taagaggaca atgcggcatg
      481 agtatacccg ctaatggact gcggagtaag tacactgccg ctcgattgct taaaccctcg
      541 ccatttatgc tgggttttta tcatttttct taatgttttc cgcacgttct gctttttggc
      601 gtgcttctgc tttgcgcttg ttgctcatgt cgttacggat ctgtgcatga ctcattaacg
      661 cgaagataaa ggtgccgccg cagatattcc ccgctaaagt aggtagtgcg aagggccaaa
      721 tgaaatcgct ccagtgcagc gtgccgttaa acaccagata gaggatttct acagaaccga
      781 caacgatgtg ggtggtgtca cccagtgcaa taagccaggt catcaatata atcaccacaa
      841 tctttgccgc acccgctgca ggaaacatcc aaaccatagt ggcgatcagc cagccggaaa
      901 tgatcgcgtt ggcaaacatc tcgctggggg tgttcttcat cacatccatg ccgattttga
      961 caaatgcatc gtgagtttct tcattgaaga taggcatata ttcaaatgcc catgcagcaa
     1021 tacctgtccc gagaatatta cccagcagca cgacgcccca taatcgcata agtaagccga
     1081 cgttgctcat tgtcggtttt tgcatgacgg gtagtaccgc agtcacggtg ttttcggtaa
     1141 ataattgctg gcgggccata atgacgataa taaaaccaaa ggtataaccg agattctcca
     1201 acaagaagct gcccggcacg ccttccagtt cgacatgaaa tatccctttt gccagtaacg
     1261 aagcgcccat cgacagaccc gccgcaatgg ctgaccacag tagtgccatt gcgtcgcgtt
     1321 ccagctcttt ttcaccatcc tggcggatat gttcatgaat tgccatcgcc cgggagggga
     1381 gtcgatcttc atctatttct atttttttgc cgcgctcttt ttcttcgctc tcaacttcaa
     1441 tttcgtcgct gtgttgatca attttgtcgt tgtccatggt ctcttctctt gaattagcac
     1501 gtatagctaa agcgtagcgg cttttttgct cgcaactggc gggagttact ctgaaaatgt
     1561 agaaaaggcc gcgtttgcct ttttctgttt ctatagaatc aagtagccta cagggcggcg
     1621 attaccaggc tatgatcaaa tcagcaaatc agggcgtctg gacatcagtt gacgtgctgt
     1681 tacaatcgcc cacacctaaa caggcggata cggtatcgtt ccgtcatgga tggcaaactg
     1741 cataagccat aaaaaaacag ggagacattt atgaagcttc gcctgtcggc gcttgctctg
     1801 ggaactacgc ttctggtggg gtgtgcgagt tccggtacag atcagcaagg gcgttctgac
     1861 ccgttagaag ggttcaaccg caccatgtac aacttcaact tcaatgtatt agacccgtat
     1921 attgttcgac cggtcgctgt cgcctggcgt gattatgttc cgcaaccggc gcgtaacggt
     1981 ttgagcaact ttaccggcaa ccttgaagaa cctgcggtga tggttaacta cttcttgcag
     2041 ggcgaccctt atcaggggat ggtccacttt acccgctttt tcctgaacac cattttgggg
     2101 atgggcggct ttattgatgt tgcagggatg gcgaacccga aactgcaacg gactgaacct
     2161 caccgcttcg gtagtacgct tggtcattat ggcgtgggtt atgggcctta cgttcagtta
     2221 ccgttctacg gtagcttcac gctgcgtgat gacggtggtg atatggcaga tggtctttac
     2281 ccggttcttt cctggctgac ctggccgatg tctgtgggta aatggacgct tgaagggatc
     2341 gaaacccgcg ctcagctgct ggattccgat ggtctgctgc gtcagtcgtc cgatccttat
     2401 attatggtgc gcgaagcgta cttccagcgt catgatttca tcgctaatgg cggcgaactc
     2461 aaaccgcagg aaaatccgaa cgcacaagcg attcaggatg atttaaaaga tattgattct
     2521 gaataagaaa caaataaaaa aggtgagtcg caaaactcac cttttttgtt gttatccctc
     2581 aattaccaca agtaaaagga gataaaccga caatgacccg gacgtaatga tgtaaaaata
     2641 ctgatgttcg tcactgacta tttccgatat tcaggcgttg aaaaatcaaa aaactggaaa
     2701 aataatttta aatatcatca aaattaacac ctggcgcaga tagttgatta atttcatttt
     2761 gatattccgt ctggcgattt tgaagccttt tttcaacttt atcttcgaga tccttaaagt
     2821 ctttgtat
//
LOCUS       DAJTSL010000270         2820 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTSL010000270 DAJTSL010000000
VERSION     DAJTSL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.273"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(113..457)
                     /locus_tag="OO433_004061"
     CDS             complement(113..457)
                     /locus_tag="OO433_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCR6052326.1"
                     /translation="MTEADLYPHLAHLAGGQVSPYVAPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(454..900)
                     /locus_tag="OO433_004062"
     CDS             complement(454..900)
                     /locus_tag="OO433_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCR6052327.1"
                     /translation="MIEILLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRTPVRRGKLRRNVVILSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(897..1217)
                     /locus_tag="OO433_004063"
     CDS             complement(897..1217)
                     /locus_tag="OO433_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6052328.1"
                     /translation="MISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGRSSRERMQSGA
                     EMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDATGGRLEILCRL
                     GGEK"
     gene            complement(1257..1583)
                     /locus_tag="OO433_004064"
     CDS             complement(1257..1583)
                     /locus_tag="OO433_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR6052329.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVLREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(1580..2179)
                     /locus_tag="OO433_004065"
     CDS             complement(1580..2179)
                     /locus_tag="OO433_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6052330.1"
                     /translation="MPAGKSLTPDDVRPMLEQMVTEAVSHIPVPRDGRDYDPDVLQKA
                     VLDAVSALPAPQDGRDATALEILPAIDVQKSFPRGTYATHQGGLWRAYEKTHGMRGWE
                     CLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTV
                     TWGGSLWHCNSMTEDKPREAHSSAWTLAAKRGRDAGGGK"
     gene            complement(2170..2820)
                     /locus_tag="OO433_004066"
                     /pseudo
     CDS             complement(2170..2820)
                     /locus_tag="OO433_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
BASE COUNT          603 a          893 c          700 g          624 t
ORIGIN      
        1 gaaatcatca cctgcgtcag ctgtgagcgt tcatacaaag cagacattct gcctcctgat
       61 aataaaaaac ccgcacgcgg cggggtatgg ttttgtagaa aaaaagaaaa agtcacaccg
      121 tgacctgaaa ctccagggtt gcacggtaac agcggttttc cggaatatag tcctgcattt
      181 cactgacgga tcccggggcc agcagcatta tggcttcacg ggcgtcctga cgtatctgac
      241 gcgcctgcgt cacagtcccg gcataaacgt ctatctgcac cgacactgag gactccgcct
      301 gcccgcccat cacgtccgct gacaccgatg aaatcaggct gaaaaccacc cacggaagcg
      361 ccaccgacgg cctgccatcc agcagggggg ccacatacgg ggacacctgc ccgccggcaa
      421 gatgcgccag atgaggatac aaatccgcct ccgtcatcgt ctcagtacct catcaatggc
      481 ccggttcatc cgcgcaatcg ccacctgagc tgcctgttca ctgcgcacat caaacgccgg
      541 gcgcacaaac gggtgcggtg gcatattcac ggtccccatt tccacaaacc gccagtagaa
      601 agcattgcgc gggttatccg ccttcatggt gttatcgctg ttaccggtgt ccggattaac
      661 accacggata tggacaccgg attccatccc gccatcgcgg gagcgccggg aaaggatcac
      721 cacattgcgg cgcagttttc ccctgcgtac cggtgtccgt gacaccactt cttctttcag
      781 cacattcgca cccgcacggg ttgcctcacg cagcacccgg ttattttccg caccactcag
      841 aagctgcaaa tcgcggctga tgtcctccag ccccgaaaaa tccagcagga tttcgatcat
      901 ttttcccctc ccagccgaca gagaatttcc agacgcccgc cggtcgcatc cggcacgggc
      961 agcccgacaa cgttcaggat ccggtcacgc catggaccac tcagcacatg aagtcgtgac
     1021 gctgccgtga tttcccgacc ggactgaccg cgcacccaga tgcggatttc cgcctgcgcc
     1081 atttccgcac cggactgcat ccgctcccgg ctgctcctgc ctcggatatc cgcatgaatt
     1141 ttcccgcatg acacccattc ttccgtcatt tctccggcag cgttacgggt taacaccggg
     1201 ttcagaacac ttatcatcta tgtcagacga cctgcagata ttgccattcc ccctcctcat
     1261 aacaccgtcg gacaacgcaa atcgtaaatc agcacggaaa cagaaaacgg cagctccccc
     1321 tgaagcagtt cttcccgctc cgcaagatcc ggattccggt acagcatccc ggtcagtcgc
     1381 atggcagccc ccttcatccg ggttaatgcc tcgcccggga tcagttcacc gtcctcacgg
     1441 agcactttat cccggctgcc ctgaatgtag gccagcagca cggcggtagc ctgacgaacc
     1501 ttgtccatca gcatgtcatc atccgcgtca tggtcgacac gcagatgtgc cttgatctct
     1561 tccagtgtca gtaatgccgt cattttccgc ctcctgcatc ccgcccacgt tttgcagcca
     1621 gggtccaggc tgatgaatga gcttctcggg gtttatcttc ggtcatactg ttgcagtgcc
     1681 acagcgagcc cccccacgtc accgtatcgc cggggtggta ggtttcaccg gctctgaaca
     1741 caccgcggta gagcatcacc ggcagggaaa atgttttttc cgtacgctgg ccactgctct
     1801 gccggaccac cacagagaac aaccgctcac ccgtcatgct gacgtcaata tccgccaccc
     1861 cgtcaaccag gcattcccat ccccgcatcc cgtgcgtttt ttcatacgcc cgccagagtc
     1921 cgccctggtg tgtggcatac gtgccccggg gaaaggattt ttgaacgtca atggcgggga
     1981 gtatttccag agccgtggca tcacgcccgt cctgcggagc cggcagggca ctcaccgcat
     2041 ccagaaccgc cttctgcaga acatccggat cgtagtcacg accatcacgc ggaacaggga
     2101 tatggcttac agcctccgtc accatctgtt caagcatcgg acgcacatca tccggggtga
     2161 gacttttacc ggcagggatg ctttcaccat atacgaaccg tttctttcgg gcgtcatggt
     2221 gacctcggct acaccctgcg tattagtaga gaccgtattc tggctgatga tcatttgcga
     2281 ggatggctct gcactgaatg tcacgggttg atgcgcaacc gggttgccaa actggtcgtt
     2341 aacgtgagct ttaactgcta cgtcttgtgc gaccattacc tgaccttccg gtatctccag
     2401 actggtaatc gtcgcagaat taacatctgc actggcattc agtaatcgcg agatgacttc
     2461 agtaggttta tctgccagag aggctgaaac tgttttcagt ccgacctcgg tgcttgtcac
     2521 aagaatttca gcgaaacccc gatcatccgt ttcaacgctg gtgctgctta gcgtgacgga
     2581 ggttccgcgg aaattaactt ttatgccttc taccgggttg ccgttagcgt cagtgacgct
     2641 tgccgtcatg gttgcgccct cggtcgtgct gacgacgaat gagtatacag aggttaagga
     2701 ggctagtttt gcggtaccag catcggcaat caaagtcacc tgtttcgtat cactgacacc
     2761 ataattgttc acgctaactg tcacggggta ggtgccggat tttgtaccgc tcagtgtaac
//
LOCUS       DAJTSL010000271         2661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTSL010000271 DAJTSL010000000
VERSION     DAJTSL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.274"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            29..865
                     /locus_tag="OO433_004067"
     CDS             29..865
                     /locus_tag="OO433_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6052331.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADGDTDSY
                     MWFETGDNGNEYFKWRSRQSTTTKDLMTLKWDALNILVNAVINGSLGVGSTNALGGSS
                     IVLGDNDTGFKQNGDGILDVYANSQRVFRFQNGVAIAFKNIQAGDGKKFTLSSSNNST
                     KNATFNLWGASTRPVVAELGNEAGWHFYSQRNTDNSVIFSVNGQIQPSNWGNFDSRYV
                     KDVRLGTRVVQLMARGGRYEKAGHAITGLRIIGEVDGDDEAIFRPIQKYINGTWYNVA
                     QV"
     gene            834..1397
                     /locus_tag="OO433_004068"
     CDS             834..1397
                     /locus_tag="OO433_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6052332.1"
                     /translation="MAHGITSHRCKLCSILKNIKSGNPKTKEQYQLTKNFDVIWLWSE
                     DGKNWYEEVNNFQDDTIKIVYDENNIIVAITKDAPTLNPEGFSVVEIPDITANRRADD
                     SGKWMFKDGTVVKRIYTADEQQQQAESQKVALLSEAESVIQPLERAVRLNMAMDEERT
                     RLESWERYSVLVSRVDTANPEWPQKPE"
     gene            complement(1426..1959)
                     /locus_tag="OO433_004069"
     CDS             complement(1426..1959)
                     /locus_tag="OO433_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6052333.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYYHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWLFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1962..2519)
                     /locus_tag="OO433_004070"
     CDS             complement(1962..2519)
                     /locus_tag="OO433_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6052334.1"
                     /translation="MAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWDSG
                     AYNALIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGVELGWSDFYTT
                     TRKPSAGDVGAYTRAECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYVVTGSVNGNRD
                     ELIDTTQARPIQYCINGTWYNAGSI"
BASE COUNT          759 a          570 c          592 g          740 t
ORIGIN      
        1 ggaaacggta aacagggctg gtaacgccgt gcaaaaaaat ggcgatacct tgtccggtgg
       61 gcttactttt gaaaatgact caatccttgc ctggattcga aatactgact gggcgaagat
      121 tggatttaaa aatgatgccg atggtgacac tgattcatac atgtggtttg aaacaggcga
      181 caacggcaat gaatatttca aatggagaag ccgccagagc accacaacaa aagacctgat
      241 gacgctgaaa tgggatgcac taaatattct tgttaatgct gtcattaatg gcagtcttgg
      301 agttggttcg acgaatgcgt taggtggtag ctctattgtt cttggtgata atgacaccgg
      361 atttaaacag aatggagacg gtattctgga tgtttatgct aatagtcagc gtgtattccg
      421 ctttcagaat ggagtggcta ttgcttttaa aaatattcag gccggggatg gtaaaaaatt
      481 cacgctatcc agctccaaca actccacaaa gaatgcaacg tttaatttat ggggtgcttc
      541 aacccgtcca gtagttgcag agttaggcaa tgaggcagga tggcatttct atagccagcg
      601 aaatacagat aactcggtaa tattttctgt taacggtcag atacagccca gcaactgggg
      661 gaattttgat tcacgctatg taaaagatgt tcgcctgggt acgcgtgttg ttcaattgat
      721 ggcgcgtggt ggtcgttatg aaaaagccgg acacgcaatt accggattaa gaatcattgg
      781 tgaagtagat ggcgatgatg aagccatctt cagaccaata caaaaataca tcaatggcac
      841 atggtataac gtcgcacagg tgtaaattat gcagcatatt aaaaaatatt aagtccggaa
      901 atccaaaaac aaaagaacaa tatcagctaa caaagaattt tgatgttatc tggttatggt
      961 ccgaagacgg taaaaactgg tatgaggaag taaataactt tcaggacgac accataaaga
     1021 ttgtatacga cgaaaataat attattgttg ccataaccaa agatgcccca acgcttaatc
     1081 ccgaaggctt tagtgtcgtt gagattccag atataacagc caatcgtcgt gccgatgatt
     1141 cagggaagtg gatgtttaag gacggaactg tggttaagcg gatttataca gcagacgaac
     1201 agcaacaaca ggccgaatca caaaaggtcg cgttgctttc tgaagctgaa agcgttattc
     1261 agccactgga acgcgctgtc aggctgaata tggcgatgga tgaggaacgc acacgactgg
     1321 agtcatggga acgctacagc gttctggtca gccgtgtgga tacagcaaat cccgaatggc
     1381 cacaaaagcc tgagtaaaaa ttaaggccca atatcgggcc ttctctcatt ctggttgttc
     1441 gggaaacgtt actggcaggc tggaggtgtc tgtagattcg actttctgcg catagagcat
     1501 ccactcggtt aatttttgtt tattctcgtc ggaaatgatg cccagccgta gctgtgagtc
     1561 ccatagctgg gttttatccc tgacaagttg caacaggctt tgcttttcat tttccgcttg
     1621 ttgcctctgc tcttcctcgg tataagttcg ctttatcact acgccatctt tgaacaacca
     1681 tttccccgaa atatcagccc ggcgatttgc tgtaatatca ggtaattcaa cgacgcttgc
     1741 gccttccgga ttaattgctg aaacatcctt ttcaatacaa ataataacgc cgttatggta
     1801 atagaccatt ttcaaagtgt ctggctggaa attcttttgt tcctcatacc agtttttccc
     1861 atcatctgaa taaagccatt tgatgttaaa ttgctttgtt agctggtatt gctcttttgt
     1921 tttagggttg ccagcagtaa tgttttttaa gtgcatcatc gttaaatact ccccgcgtta
     1981 taccacgtcc cattaatgca atactgaatt ggccttgcct gtgttgtatc aattaattca
     2041 tcacggtttc cgttaactga acccgtaacg acataacctg acctgtcaga ccagccggga
     2101 ccattccatg tctgaacaga tgacagaccg ccaaggcgaa tacctgtaat aaaccttgag
     2161 ttacattctg cccgcgtata tgcaccaaca tctcccgctg aaggttttcg tgtcgtggta
     2221 tagaaatctg accaacctaa ttcaacccca taaccatcac gagccgacct gtaggaaatg
     2281 ccaccatttt tataattcac acggaattgt acggcaggac agctaccagc atttatatta
     2341 aagtgaagaa ttaatgcaga tgcaccacca atcagggcgt tatatgcacc actatcccaa
     2401 ttccatccaa cggatgtatc accattaaca gtattacctg tttgtcggag tgcaaatgca
     2461 ttgacatttt tggcactaat tgaaatatcg ctagaaccat caaaaccaac tccagcaatt
     2521 tttcgtgctg ttttcagctt tgttgcagta gcggcattgc cggacagttc cccagaaagg
     2581 ccaccgctga atgtttgtcg attggtccag gtattcgctg tactgagtaa cggtattttc
     2641 tccccgcttg tgccgagttc t
//
LOCUS       DAJTSL010000272         2603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTSL010000272 DAJTSL010000000
VERSION     DAJTSL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.275"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..484
                     /locus_tag="OO433_004071"
     CDS             <1..484
                     /locus_tag="OO433_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6052335.1"
                     /translation="DIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVS
                     YVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIY
                     QPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV
                     "
     gene            complement(504..755)
                     /locus_tag="OO433_004072"
     CDS             complement(504..755)
                     /locus_tag="OO433_004072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052336.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            678..1368
                     /locus_tag="OO433_004073"
                     /pseudo
     CDS             678..1368
                     /locus_tag="OO433_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1385..1564)
                     /locus_tag="OO433_004074"
                     /pseudo
     CDS             complement(1385..1564)
                     /locus_tag="OO433_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1894..>2603
                     /gene="ipaH7.8"
                     /locus_tag="OO433_004075"
     CDS             1894..>2603
                     /gene="ipaH7.8"
                     /locus_tag="OO433_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6052337.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLS"
BASE COUNT          770 a          593 c          521 g          719 t
ORIGIN      
        1 ggatatccat cttcatccca aaatcggtgc ggactggcaa ctgcgcggac agcaaaaacg
       61 ggtggtcacg ccgggacaga atgaaaaata ttatctggcc ggagcgctgc acagcgggac
      121 aggtaaagtc agctatgtgg gcggcaacag caaaagttcg gcgctgttca tcagcctgct
      181 gaagcggctt aaagcgacat accgtcgggc gaaaaccatc acgctgatcg tggacaacta
      241 cattatccac aaaagccggg aaacacagag ctggctgaag gagaacccga agttcagggg
      301 catttatcag ccggtttact cgccatgggt gaatcatgtt gaacggctat ggcaggcact
      361 tcacgacaca ataacgcgta atcatcagtg ccgctcaatg tggcaactgt tgaaaaaagt
      421 tcgccatttt atggaaaccg tcagcccatt ccccggaggc aaacatgggc tggcaaaagt
      481 gtagcggtat taagcgcagc tatttagatt attgccgcat tttctccacc acgatcagag
      541 ccgaaaaaag ataatttttt tccaactgcc acccggcgta atgcgttttc accaatgtta
      601 ccccacctat tgcggcaaaa ggtaatgtct tcaatcgata gaagtttctg gtaatctact
      661 ggtatttgag gttttccatg actaccgtta ctgttcattg tccgcgctgt aattccgatg
      721 aagtttatcg acatggccgt agttgttccc ggcatgagcg tttccgttgt cgctcatgta
      781 aacgtgtttt ccagctcact tactcttatg aagccagaaa actcggcgtt aaggagcaga
      841 tcgttgaaat ggcacataac ggcgcagggg gctgttatac cgccagaaca ctgaaaatcg
      901 gcatcaatac cgtcatacgg acattaaaaa gctctcgccc cggcggataa catctgcgcc
      961 agtcgctcac gctgatgttg cccttatctg tgaacttgat gagcagtggc gcttcgttgg
     1021 cagcaaggcg caacaacact ggccctggta tgcgtacaac actaaaactg gcggtgtgtt
     1081 ggcttacact tgcgggccac ggaatgatga gacctgtcgg gaactgttag ccctgttgac
     1141 tccgttctgt atcggcatgg tgaccagcga tgactggggc agctacgcca gagaagtgcc
     1201 ggaggagaaa cacctgaccg gcaaaatctt cacgcaacgt atgaacgtaa caacctgacc
     1261 ctccgccccc gcatcaagcg gctggcacgt aaaacaatct gcttctcgcg ttccgtggaa
     1321 atccacgaaa aagtcatcgg ttccttcatt gaaaagcaca tattctaatg attggaagta
     1381 ctacccggca aaactcattc agtgcattcc agtgattcag gatatagttg aacgccttta
     1441 ccatttcagc atggtttgac aaagttttcc tctggagctt tatccagtca tacaaggact
     1501 tcattcgcgg cacacatttc tcctgcctta ccgcaagccg tttttctgcc ggcctccctc
     1561 atattaaatg tgtatcgttt ttttacagta attttaattt atcattctat aataggaata
     1621 aatgagcttg gctaaaatat gatgtaaaac aaatatactt actcggatta catttccttg
     1681 ctcactggaa tgttgaattc agtgttatta ttgactgcag tcacaataaa aaaaagaatt
     1741 ccatagaaaa cctcaacaaa taaattacaa ctaacttctg ttatgtgtaa aatggaaact
     1801 attaaaactt aatatcgaaa atggtaagtg aaatttgtat aaatatacaa ttttaaatat
     1861 ttattctcac aaatataagg ttgccctagc attatgttct ctgtaaataa tacacactca
     1921 tcagtttctt gctccccctc tattaactca aactcaacca gtaatgaaca ttatctgaga
     1981 atcctgactg aatgggaaaa gaactcttct cccggggaag agcgaggcat tgcttttaac
     2041 agactctccc agtgctttca gaatcaagaa gcagtattaa atttatcaga cctaaatttg
     2101 acgtctcttc ccgaattacc aaagcatatt tctgctttga ttgtagaaaa taataaatta
     2161 acatcattgc caaagctgcc tgcatttctc aaagaactta atgctgataa taacaggctt
     2221 tctgtgatac cagaacttcc tgagtcatta acaactttaa gtgttcgttc taatcaactg
     2281 gaaaaccttc ctgttttgcc aaaccattta acatcattat ttgttgaaaa taacaggcta
     2341 tataatttac cggctcttcc cgaaaaattg aaatttttac atgtttatta taacaggctg
     2401 acaacattac ccgacttacc ggataaactg gaaattctct gtgctcagcg caataatctg
     2461 gttacttttc ctcaattttc tgatagaaac aatatcagac aaaaggaata ttattttcat
     2521 tttaatcaga taaccactct tccggagagt ttttcacaat tagattcaag ttacaggatt
     2581 aatatttcag ggaatccatt gtc
//
LOCUS       DAJTSL010000273         2593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTSL010000273 DAJTSL010000000
VERSION     DAJTSL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.276"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..381
                     /locus_tag="OO433_004076"
     CDS             <1..381
                     /locus_tag="OO433_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6052338.1"
                     /translation="CRGAILVDSKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKV
                     PLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIR
                     SNNCYGQVSRRDQESALACWDIDR"
     gene            537..719
                     /locus_tag="OO433_004077"
     CDS             537..719
                     /locus_tag="OO433_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052339.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            1046..1198
                     /locus_tag="OO433_004078"
     CDS             1046..1198
                     /locus_tag="OO433_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001228697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HCR6052340.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPEWIMQPAPDWQTPLNGII
                     SPSESG"
     gene            1284..1508
                     /locus_tag="OO433_004079"
     CDS             1284..1508
                     /locus_tag="OO433_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052341.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            1853..2179
                     /locus_tag="OO433_004080"
     CDS             1853..2179
                     /locus_tag="OO433_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HCR6052342.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(2311..2511)
                     /locus_tag="OO433_004081"
     CDS             complement(2311..2511)
                     /locus_tag="OO433_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR6052343.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
BASE COUNT          637 a          563 c          703 g          690 t
ORIGIN      
        1 tgccgcggtg ccatcctggt ggatagtaaa cctgtcgtcc cgggcatgaa gttgtcgaag
       61 gaaaaatgcg accaggtcaa cgccattgag cgtgataagg cgctggcatg ggtggagcgc
      121 aatattaaag tgccgctgac cgaaccccag aaagcgggta tcgcgtcatt ttgtccctat
      181 aacattggcc ccggtaagtg tttcccgtcg acgttttaca gacggattaa tgctggtgac
      241 cgcaggggag catgcgaagc gattcgctgg tggattaagg acggtggcag agactgccgt
      301 atccgctcaa ataattgcta cggtcaggta tcccggcgag accaggaaag tgcgctggcg
      361 tgctgggaca tcgacagata agcagaatat tttgctgaaa ataaggcatg agcacgcgga
      421 cggataacac gaaatcctgc gaactggcga aacgtaagtg aataaaagta aaaaccccgt
      481 ttgttggcac caggcggggt tttgtgtttc tgaccttgag taaggcaagg gagaacatgg
      541 cgaagtataa acgaattctg ttgaggttga ccatgaaaaa cggccttgaa ctgaaagcgc
      601 ctgtaactga tgacatcagc agagcactgg cttttgccat taagtgggtg gcggtcggtg
      661 ttgctgtgtc cccgatgctg tatgggctgg caaaactggt cattgcgttg aaatcgtgaa
      721 gggaggatta agcatgtcag acaaactcat aacgctggcg aagatcctct gtgtaattgt
      781 cggcatttca ttttcactaa tgctggttgc tctttttctt tccatggcct ggatgatgtt
      841 gtcttcgtcg gggctgctgg ggtgaacata aaccgaatgc tttccgcgtt taccgttatt
      901 ctgctggtgg tctgtggtgc gctgtggctg gcaacagacc attaccgtga taacgccatc
      961 acctacaaag cgcagcgcga taaaaaagcc agagagctgg agctggcaaa cgcaaccatt
     1021 actgacatgc agcagcgcca gcgtgatgtt gccgctggtc gtaagcgcct gcggatcaac
     1081 gccacctgct ccggtaccgt gcgtgaagcc accggcacct ccggaatgga taatgcaacc
     1141 ggcccccgac tggcagacac cgctgaacgg gattatttca ccctcagaga gcggttgatg
     1201 acaatgcaga agcaactgga aggggcgcag gaatatatcc gcactcagtg tattaactag
     1261 tatttttgtt atccggagaa tgcatgaaga aattactggt aaccgtaaag ccttttcagg
     1321 gaacaattcc gttccgtatt ttgcagcgtg gtcgtgttct tgttgaaggt tcgttcagtg
     1381 gtaaatgtac gcaattacac tcccggacct ttcaggtgaa tgccacgaat gaagagctaa
     1441 ccgttgagtg tacgatgaat gccgctaaat gccgcatggt atccgctgca ttacagccag
     1501 tgtgttgagc gaccttatta accatgcgcg gtattgtcgc cgtatccccg cattaacaga
     1561 gaccgcagcc cgaccgggag actcctctgc gcgagtgtgc ggggataatc aaaaacgata
     1621 cacaccgggg tttaccgcgt taacggagcg cggcgttgtc ccctcatggt cgctggtccg
     1681 gtgcgatggt ggaagaaacc ggacgatgtg ttacctcgca agctctgtta tgtcatgtgt
     1741 ctgatttgtg atttaagtcg gataattgtc gttgccatta agcagaggat tgatgaccga
     1801 cagggtggca ttgttagaat aagacttatt cttatctgtg ccgggaatga aaatgaaaag
     1861 aaaccttccg ttaattattt tgttgtcttc tctggttatg ggctgtacgc aacataaaac
     1921 agatatgccc cgacagttgg ttaaggcatt accacaatat ccggcctatg cagcggcaaa
     1981 ttatataaag ggacgggttg atgtgaagtt tgatattggt gctgatggta ctgtcacccg
     2041 aattgagttt atccgttcag agccgcacca tttgtttgat gagcaggttg taaaggcgat
     2101 ggcaaaatgg cgatttgaga aggacaggcc gtgtaaaggc gtgaagaaaa cgtttatctt
     2161 tagtccttct gcaccctgat tatttcatca gaaattaatt atcactctgt tgttattctg
     2221 tacatcccgg cagggtaagt cttgttccgt cggatatgaa gatgaaatat tgttggagga
     2281 cagtgggtac ctgctcctgt aaccgaacgt tcatttctcg ttatttttca tgctggccgg
     2341 gcgcagatgc gttgcatctg ttgccagcct tctcctgcag gcttcaataa cccacgctga
     2401 aaagtttcct gaacctttca gatcaagagc gatgttaatt tgttcaatca tctggtttgg
     2461 aaatcggatg ttgcgggttg ttgttctgcg ggttctgttc tttgatgaca taatgtttcc
     2521 ccatattcag tgttgctgat ttgtattatc tgaagttgct tttacgttaa tttgatgcag
     2581 atcaatcaat acg
//
LOCUS       DAJTSL010000274         2590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTSL010000274 DAJTSL010000000
VERSION     DAJTSL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.277"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..84
                     /locus_tag="OO433_004082"
     CDS             <1..84
                     /locus_tag="OO433_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6052344.1"
                     /translation="VLDYIDAVAATDTSTAPDVIWPELPEA"
     gene            complement(56..493)
                     /locus_tag="OO433_004083"
     CDS             complement(56..493)
                     /locus_tag="OO433_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6052345.1"
                     /translation="MFYSPSLNIFVNPALKDDYINANSWPDDALAVSDDVYNEFAINT
                     PPDDKIRVAGKNGLPTWALIPPPSYEELIQQAESERQLLLNQANEYMNSKQWPGKAAI
                     GRLKGEELAQYNLWLDYLDALELIDTSSAPDIEWPTPPAVQAR"
     gene            complement(495..881)
                     /locus_tag="OO433_004084"
     CDS             complement(495..881)
                     /locus_tag="OO433_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6052346.1"
                     /translation="MPVSHYLCIFLNVGLGELSLAGTASGVIGLNGYVTIPLIISGSR
                     RTLIIQWGQARFGGSGGEDAGYLNDFPFAFPSACYGMIVSHVGHTPSGAGILSASAIT
                     SNQFRGFSSIATAANAVLGRYIAIGV"
     gene            912..1478
                     /locus_tag="OO433_004085"
     CDS             912..1478
                     /locus_tag="OO433_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6052347.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKPDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANESNESP"
     gene            1621..>2590
                     /locus_tag="OO433_004086"
     CDS             1621..>2590
                     /locus_tag="OO433_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HCR6052348.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDITTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAK"
BASE COUNT          675 a          638 c          666 g          611 t
ORIGIN      
        1 gtgttggatt acattgacgc ggtggcggca acagatacca gcaccgcgcc ggatgtcatc
       61 tggcctgaac tgccggaggc gtaggccatt caatatctgg agcactggag gtatcaatca
      121 gttccagtgc gtccagataa tccagccaca aattatattg cgccagttcc tcacctttca
      181 gacgaccaat agcggcttta ccgggccatt gtttactgtt catgtattcg ttggcctgat
      241 taagcaataa ttgcctttct gattctgcct gttgaataag ttcttcatat gatggtggag
      301 gtattagtgc ccatgtgggt aatccatttt ttcctgcaac acgaattttg tcatctgggg
      361 gcgtattgat tgcaaattca ttataaacat catcactgac agccagagca tcatctggcc
      421 atgaatttgc attaatgtaa tcatccttaa gcgcaggatt cacaaaaatg tttaaagatg
      481 gactataaaa catattaaac ccctatagcg atataacgac ctaatacagc atttgcagca
      541 gtcgctatgc ttgaaaaacc gcggaactga tttgatgtaa ttgcagaagc cgacaggatt
      601 cctgcgcctg aaggtgtatg ccccacatga ctaactatca tcccataaca tgctgacgga
      661 aaggcaaaag gaaaatcatt aagatatcct gcatcttcac caccagaccc accaaatctc
      721 gcctgccccc actgaataat cagtgttctc cgggaacctg aaataattaa cggaatcgtt
      781 acatacccat tcaggccaat gacacccgat gcagtgccag ccagagataa ttctcccaaa
      841 ccaacgttta agaaaatgca gagataatgg ctaactggca tcatccccgg tttttattca
      901 ggggattgat catgcttatt ggctatgtac gcgtgtcaac aaatgaccag aacaccgatt
      961 tgcaacgtaa tgcactgaac tgcgcgggat gtgagcggat ttttgaggat aaaatcagtg
     1021 gcactaagcc cgacagaccg gggcttaaaa aactgctcag gacactatcg gcaggagaca
     1081 cgctggttgt ctggaagctg gacagactgg gacgcagtat gcggcatctt gttacgctga
     1141 tagaagagtt gcgccagcgt ggtgtgaatt tccgaagcct gactgacagt attgatacca
     1201 gtaccccaat gggccgtttc ttttttcacg tcatgggggc tctggctgaa atggaacgtg
     1261 aactgatagt tgaacgtacc agagcagggc tggctgcagc tcgtgccaaa ggcagagtag
     1321 gtggacgccg tcctaagttg accaccgaac agtgggcaca gattggacgt ttactcgagg
     1381 ccggagaatc aagacagcgt attgcactga tttttgatgt gggcgtttct accatttata
     1441 gaaaatttcc ggcgaatgag agcaatgaat cgccctgagt cagtattatt ttgatttttc
     1501 ctgtaagcgg acaaataccg tcattttgtg tgaataacga cacaactgcg cttagctgtt
     1561 tgtcaggcac aatcacttca acatagggcg aagcctaatc caatcaggag gttcgccact
     1621 atggctcagg attaccacca cggggtgcgc gttgttgaag tcaacgaagg cacccgatcc
     1681 attaccacgg tgagcaccgc catcgtgggt atggtctgca cgggcgatga tgccgatgca
     1741 aaaatgtttc ctcttaataa acctgtgctg atcactgatg tgctgaccgc cagcggtaaa
     1801 gcgggtgagt ctggcacgct ggcccgttcg ctggatgcca tcgctgacca ggcaaaaccc
     1861 gtgaccgttg ttgtgcgtgt gccgcagggt gaaacggaag aagaaaccac aaccaatatc
     1921 attggcgcag tgaccgccga gggtaaaaaa acaggtatga aagccctgtt atctgcccag
     1981 tcacagctcg gcgttaaacc gcgcattctc ggcgtgccag gtcacgacac gaaggcggta
     2041 gctactgagt tgctgagcgt ggcgcaaagc ctgcgtggat ttgcttacct gtcagcgtat
     2101 ggttgcaaga cggtgcagga agcaatcact taccgtgaaa acttcagtca gcgtgaagga
     2161 atgctgatct ggcccgactt tactggctgg gacacggtgc tgaatgccga agcaacggct
     2221 tatgccaccg cccgtgcgct cggtctgcgc gctaaaattg atgagcagac cggatggcac
     2281 aaaagcctgt ccaacgtggg cgtgaacggt gtcaccggaa tttctgctga tgtgttctgg
     2341 gatctgcagg acccggcaac cgatgcaggt ctgcttaacc agaacgatat caccacgctt
     2401 atccgcaaag acggtttccg cttctggggt tcccgctgtc tgagcgatga tccgctcttt
     2461 gccttcgaaa actacacccg cacggcgcag gtgctgatgg acacgatggc agaagcgcac
     2521 atgtgggcgg tggacaaacc gctgaacccg tcgctggccc gcgacattat cgaaggtatc
     2581 cgcgccaaaa
//
LOCUS       DAJTSL010000275         2451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTSL010000275 DAJTSL010000000
VERSION     DAJTSL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.278"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            545..1084
                     /locus_tag="OO433_004087"
     CDS             545..1084
                     /locus_tag="OO433_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6052349.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(1310..1633)
                     /locus_tag="OO433_004088"
     CDS             complement(1310..1633)
                     /locus_tag="OO433_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6052350.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1917..2111
                     /locus_tag="OO433_004089"
     CDS             1917..2111
                     /locus_tag="OO433_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6052351.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
BASE COUNT          834 a          577 c          512 g          528 t
ORIGIN      
        1 ttatcgccac tggcagcagc cactgataac cgaattagag gagtaagact tgaagtcatg
       61 cgcctgttaa ggctaaactg aaagaacaag ttttggtgac tgcgctcctc caagccagtt
      121 acctcggctc aaagagttgg tatctctgag aaccttcgaa aaaccgcctt gcagggcggt
      181 tttttcgttt acaggcagta gattacgcgc agaaaaaaag gatctcaaga agaaccttta
      241 attttttttc acacttggta tcctacttta acccataaaa taaatactta caccgacacg
      301 cccttaaaca aaagaaaaaa aaacaataaa aaacaccgca aaaatggatt gttaacaaat
      361 gaaaatactc aattcgaatc tcataacaaa catatcatca acaataagaa atcaccttct
      421 aacatcatcc aaaaatataa aaacacaccc atcaccaaac atggacgtaa caagtcatcg
      481 tcgtttattc cagaaagacg gaataggatt gagccaaaca ggaaaagcaa cacaacttgt
      541 cattatgtcg catggtggat ggaaagagat ctcccatcca caaacactat tcacgcgtca
      601 aaaaggcgat ggatggactg tagtccccaa aaacctaaga attgattttt atacaaaaga
      661 caacgacttc acaaagggac taagcgttct ttcagaggta aacaaacgcc atactgaagc
      721 tcaaaaaggg ttaacaccat cactaaacat aagcaaagat gacctaaaag ttctagcgaa
      781 cgcgcgcaat atacctcaat caaaattaga agaagaaatg atgtcacaag ccatatatag
      841 aaaagaatac gcaacaagta atgagaagat aaaaaactat gctctatatt accatgaaca
      901 agcccaaaat attatacaaa gacatcaaga tggcttagga aacaaaaata ttgatatagc
      961 atttataact gataaaaatc ataagaagca cctttctgac atatttaaag taataaatga
     1021 atcaggagta aaatatgatg taattcactt tggcgcttgc cgagtaaata gagaggagaa
     1081 ataatcatca tttaccataa tcccttaatt gtacgcacca ctgaaacgcg ctgaacgcga
     1141 ttctgacgca gacaccacaa aaatagatgt tttaccccgg aaaaacagga aggatgcgtc
     1201 aggatcgttt tcagctcgct gcatagctat gcatgaaagc gaatggagat cactttggga
     1261 gcttaccgtg ttcataccgt ctgttttcaa cagttttctc cccgggaagc taatctgcca
     1321 taagcctgga taacagggca cggtgatact ccgtaatagc gatcagcacc tccgcatact
     1381 ccctgtcccc gatacgcttg ccgtcgataa agagtttttt ctgtaacgcc cccagtctca
     1441 taacttcttt cagtacccga tcatcagtca gtgagttaac gttcttaccg agagctgccg
     1501 cccgtaaaaa accggaaacg gtcttgccgc attcagcagc cctttttctg atttcctggt
     1561 cttcttctgc cgtcagacga acagggcgac tgatagcccg cctgcgcgta ttacttccac
     1621 ttcgttttgt cataaaacat ggctccgtat ctgacatggg tgtcggggca aagccctgac
     1681 cagggcaatt gtaatagcgt gcatgtatgc gcggtataac aattgcacat cctgtcctgt
     1741 cagcaagctc gaattaccga caaaggcacg gcagtttttt ggcagttaca gtgttgtctg
     1801 acagaaatgc ccgtcaaaaa gccaggcggc agaatagcgg cagcaaaaca gtcgaaaagt
     1861 ggtacgcgat ataatcacat aacgcagatg acagacagaa cggagaaaac gaaaagatga
     1921 acaagcagca gcaagccgta ctcaacatgg cgggattcat aaaaagccag agcctgacgc
     1981 tgctcgaaaa actggacgca ctcgatgctg acgagcaggc caccatgtgt gagaaactac
     2041 acgaactcgc agaagaactc cagaacagca tacagacacg ctttgaagta gaaaatagca
     2101 cagagagata acgctcagga caggatgtcg aacggcacaa cagagcgtaa ggagaaaatg
     2161 tcacataagg cgctcttccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
     2221 gcggcgagcg gtatcagctc actcaaaggc ggtaatgcgg ttatccacag aatcagggga
     2281 taacgccgga aagaacatgt gagcaaaaag ccgaaggcag ggacaaaggc cgcgcaggtg
     2341 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2401 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg a
//
LOCUS       DAJTSL010000276         2448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTSL010000276 DAJTSL010000000
VERSION     DAJTSL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.279"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..2139
                     /locus_tag="OO433_004090"
     CDS             <1..2139
                     /locus_tag="OO433_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6052352.1"
                     /translation="KDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQIALYRDARGY
                     VSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPWRLRNYSTWTR
                     NDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLASDDNMLPNSQ
                     RGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSGDLEVTIEESD
                     GTQRRFIQPYSSLPMMQRPRHLKYSATAGRYRADANSDSKEPEFAEATAIYGLNNTFT
                     LYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGYQWRTQYIKDI
                     PETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTIFDGVSLYASG
                     SQQDYWGNNEKNRNISVGVSGLQWGIGYSLNYQYSRYTDENNDRALSLNLSIPLERWL
                     PRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHNSSLNASYRSP
                     YGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDANGASGVRIQNY
                     PGIATDPFGYAVIPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNA
                     NIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISSKSQSWTVRWG
                     NQADQQCQFAFNTPDSEPTTSVLQGTAQCH"
     gene            2150..>2448
                     /locus_tag="OO433_004091"
     CDS             2150..>2448
                     /locus_tag="OO433_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6052353.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL"
BASE COUNT          672 a          598 c          572 g          606 t
ORIGIN      
        1 aaagatgacg atagcgtgat caactcgctt gaacaaatca ttcccggtac agctgctgaa
       61 tttgatttca atcatcagcg acttaatttg agcattcccc aaattgcact gtaccgtgat
      121 gcaagaggtt acgtctcccc ttctcgttgg gacgatggta taccaacgct gtttaccaac
      181 tactcgttta caggttctga taaccgttac cgccagggca atcgtagcca acgacagtac
      241 ctgaatatgc aaaatggtgc taattttggc ccctggcgat tacgtaacta ttctacgtgg
      301 acacgcaacg atcaggcgtc aagctggaac actatcagta gttatttaca acgtgatatc
      361 aaggcgttga agtctcagtt gcttctggga gaaagcgcca ccagcggcag tattttttcc
      421 agctacacct ttactggcgt gcaactcgct tccgacgata atatgttgcc aaacagccag
      481 cgcggatttg ccccaacggt acgcggtatc gcaaacagta gtgcaatcgt gactatcagg
      541 caaaatggtt atgtgatcta tcaaagcaac gtgccagcgg gtgcctttga aattaacgat
      601 ctctacccct cttccaacag cggcgattta gaagtcacga ttgaagaaag tgacggtaca
      661 caacgtcgct ttatccagcc ttattcttca ttacccatga tgcagcgacc taggcatcta
      721 aaatatagcg cgaccgctgg acgctatcgc gctgatgcaa acagtgatag caaggaaccc
      781 gaatttgctg aagccacggc aatatatggt ttgaataata cttttacgct gtatggcggc
      841 ctgctcggtt ctgaagatta ttatgcgctg gggatcggta tcggcggcac acttggcgca
      901 ctgggcgcgt tgtcgatgga tatcaacaga gctgacaccc aattcgataa ccagcactct
      961 tttcatggct atcaatggcg tacgcagtac atcaaagata tcccggaaac caacaccaat
     1021 atcgctgtta gctactatcg ctataccaac gatggctatt ttagttttga tgaagccaat
     1081 acccgcaatt gggactataa cagtcgccaa aaaagtgaaa ttcaattcaa catcagccag
     1141 acaatatttg atggggtaag tctgtatgcc tccggttcgc agcaagacta ttggggcaat
     1201 aacgagaaaa acaggaatat ctctgttggg gtttccggcc tgcaatgggg aattggttac
     1261 agcctgaatt atcaatacag ccgctacact gatgaaaata atgaccgtgc actctctttg
     1321 aatctcagta ttccgttaga acgctggtta ccgcgtagcc gggtttccta tcagatgacc
     1381 agccagaaag atcgcccaac ccaacatgaa atgcgtcttg atggctcact gctggatgat
     1441 ggtcgcctga gctatagcct ggaacaaagt ctggatgacg ataacaacca taacagtagc
     1501 ctgaacgcca gttaccgttc accttatgga accttcagtg ccggatacag ttacggtaat
     1561 gacagcagcc aatacaatta cggcgttacc ggcggcgtgg ttatccatcc tcatggcgtg
     1621 acgctctcgc aatatctggg caacgctttt gcgcttatcg atgctaacgg ggcttctggc
     1681 gtgaggatac aaaactatcc ggggattgct actgatccct ttggctatgc agtgattcct
     1741 tatctcacga cttatcagga aaaccgtctc tcggtagata ctacgcagct gcccgataac
     1801 gtcgatcttg aacaaacaac acagtttgtg gtgcccaaca gaggtgcaat ggtagcggcg
     1861 cgtttcaacg ccaatatcgg ttatcgcgta cttgttacag tcagcgatcg caacggtaaa
     1921 ccgttgccct ttggcgctct tgccagcaac gatgatacgg ggcaacaaag tatcgtcgat
     1981 gagggcggca tactatatct ctctgggata tcgagtaaat cacaaagctg gactgtacgc
     2041 tggggaaatc aggcagatca acaatgtcag tttgctttta atacaccgga ttcagaacca
     2101 acaacctctg tattacaagg cacagcgcag tgccattaag gataaaaaaa tgaaaagagc
     2161 gcctcttata acaggacttt tgttgatatc cacatcctgc gcttatgcct cctcaggagg
     2221 gtgtggagcc gacagcacta gcggtgcgac aaattacagc agtgtggttg atgatgttac
     2281 ggtgaaccag acagataacg tgacaggacg ggagtttacc tctgcaacgc taagtagcac
     2341 taactggcaa tacgcctgtt cctgctctgc gggtaaggca gttaaacttg tctatatggt
     2401 cagccccgta cttaccacca ctggacatca gacaggatat tacaaact
//
LOCUS       DAJTSL010000277         2444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTSL010000277 DAJTSL010000000
VERSION     DAJTSL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.280"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..544)
                     /locus_tag="OO433_004092"
     CDS             complement(<1..544)
                     /locus_tag="OO433_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6052354.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIW"
     gene            complement(545..2044)
                     /locus_tag="OO433_004093"
     CDS             complement(545..2044)
                     /locus_tag="OO433_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6052355.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAERGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRITNPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(2105..>2444)
                     /locus_tag="OO433_004094"
     CDS             complement(2105..>2444)
                     /locus_tag="OO433_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6052356.1"
                     /translation="QPSITRAEALTTAQNIMQANANLNGLFGFGDDAALAAVIAAKSA
                     HNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDHLNGKPVEKMI
                     PVQPGVYTGK"
BASE COUNT          676 a          600 c          483 g          685 t
ORIGIN      
        1 tccagattgg aataggtata tagagaaatt ctccattccc cagtgccaga aaggcatcat
       61 ctttaatcat gatgggcata ccatcggtca ctatataagc gataccacgg aagatcccca
      121 tagtagcaac ggtgacaata aacgacggga tccccgctat agccgtcaag ccaccattta
      181 tcatccccag agccagtgct gcaataatgg ataatgggac caataaaata aatggaattc
      241 cccatgaagc cccaagcgca gcaaatgtgc caatcaatgc aatggttgaa ccgaccgata
      301 aatcaatatc gccaagcaaa agtacataag tcattccata ggcggtaatt gcaattatcg
      361 aaacctgaag aataatatta gtaatgttgt tggcggtaaa aaaactgtca ttaataaaac
      421 tgaacagaat gattaacacc accattccac tgaggatacc gccatattgc ctgaagactt
      481 ttttaaaatt atttgcaatg ggaatttccg aaggtttcgg actggcaaga gttttctctg
      541 tcatttaacg tactcctgtt gcagcggtca ttacctgtgt ctgcgttaaa ttttcacatg
      601 aataaatatc ggcaatcgtt ttattacgca ttaccaccag acgatcgcta agtgccatga
      661 cttctggcag ttcagaggac accagaataa ttgctgtccc tgccgcagcc agttgacgta
      721 tcacgttata gatttcaaat tttgcgccga catccactcc gcgcgttggc tcatcgagca
      781 ataaaatacg cggagttttc tccagccact tcgataacac gattttctgt tgattaccgc
      841 cgctcagcgc tcccactgcc tgatgtacag aactcacttt agtgttcatt ctggcaattg
      901 attgcagcgt cttcgcctct tcttgtcggt gattgatgag tcccatccgc gaagcgttat
      961 attgaaacgc cacatggtgc gtgttcatat tgacgtcgtg catcagcact aacccttctt
     1021 ccttgcggtt ttcggtcaca aaagcaatgc catgatcaat agcctgttta ggattagtga
     1081 tcctgacttc cttaccatca atcaacacag tcccctgata cgaaacgagg ccaaagagcg
     1141 ccttcatcac atcggaacgc ccggccccca ccaggccaaa aaatccgagg acctcccccg
     1201 gacgcacagc gaaactgatc tctttaaaaa gcgaaggatg ggaaagattt tttacttcca
     1261 gttttgcagg aacattttca tccggtattt ccccaagacg tgctggccaa atatttttca
     1321 tttcccgacc aaccatttgg gcaatcaaat gctggatatc actgtttttc ttttcatgcg
     1381 tggcgatata ctcaccgtcg cggatcaccg aaatatcatc tgaaatccgc ataatttcat
     1441 ccatgcgatg agagatataa ataaccgcac agcctcgctg ctttaatttt tcaataatgt
     1501 taaacagaat ctccgcttca ttatcactta gcgaagatgt cggttcatcc ataatcacga
     1561 catccgctgc atagcttaag gctttagcaa tttctaccag ttgcctatgt gccatcgaca
     1621 tattgccgac catttctagt gggtctagtg gtatgccccg ttccgcgagg agattctggc
     1681 aatctgcgag cattttttta tcgttaataa tgccaaaacg tcgcggttca ttattggcat
     1741 aaatattttc cgccacggtc atattattac tcaggctcaa ttcctgaaaa ataatcgcaa
     1801 ttcctaactg gcgtgaatgc tgcggattat taatggtgat agtttcacca cgtagtgtta
     1861 tcgttccctg ggttggcgga taaacaccgg ccagtatttt cattaatgtc gatttaccgg
     1921 caccattctc gcctaataaa gtatgcaccc gccctttacg taaagagagg gtaatatctt
     1981 ttaatgcttg aacatgacca aatgtcttcg caatatgcga aacatcaaga ataacatccg
     2041 tcataatgac ctcggtgtca caacaaaacg ccaataacgt tttgttgtga ggtgaagatg
     2101 tttactattt acctgtataa acgccgggtt gtaccgggat cattttttct accggtttac
     2161 cattgaggtg atcaacagcg gcatcaatgg ctttagcacc catttgatcg ggatattgcc
     2221 gaatcacggc cgcaaacgtt ttttcactgt caacggcatc acgcgcttct ttcatgccat
     2281 caaaaccaat gattttgatg ttgtcgttat gcgcagattt tgctgcgata accgctgcca
     2341 gcgccgcatc atcaccaaag ccaaacagac cattcaggtt ggcatttgcc tgcataatgt
     2401 tttgcgcagt cgttaacgct tcagcccgcg taatactagg ctgt
//
LOCUS       DAJTSL010000278         2434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTSL010000278 DAJTSL010000000
VERSION     DAJTSL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.281"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(73..864)
                     /gene="kdgR"
                     /locus_tag="OO433_004095"
     CDS             complement(73..864)
                     /gene="kdgR"
                     /locus_tag="OO433_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6052357.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            1041..2414
                     /locus_tag="OO433_004096"
     CDS             1041..2414
                     /locus_tag="OO433_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6052358.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
BASE COUNT          514 a          643 c          571 g          706 t
ORIGIN      
        1 ccttcaactt tatttttctc atgctaaata aagaacctgt ctgtttgata ttccgacagg
       61 ttcttactct catcagaacg gatagtcgtg ataacccatt tgggcagaaa ttttgcgcgc
      121 tgcggtatgc aacattgcga catattcctg taaacgctct tcagagaaac gcaacgtcgg
      181 gaaggagatg ctcaaaccgg caatgaccac gccaaagcga tcaaataccg gtaccgcaat
      241 gcatcgcagc ccttcttcct gctcttcgtt atcttcgcca tacccctgct cgcgcacctg
      301 gtccagaacg ggtaataacg cttctgtact ggtgatggtc cgctcggtac tgcgtttata
      361 ctccacgccc tcaagaattt gcttcacttc atcgcgatcg cgccatgcca gcagtacctt
      421 accaatcgcg gtgctgtaca gcggattacg acgcccaatc cgtgaataca tgcgcaaatt
      481 gtacatagag tcaattttgt gaatgtaaac aatactgtct tcgtccagtg cgccgaggtg
      541 gatagtttct ttggtcaggc gggagatctc acgcatctgg atatctgcgc tacgaattaa
      601 atcgacgttt tgtaacgcgc gagcgcccag ttcaaacaat ttcagggtca gggaatattt
      661 ctccgactcc ccttcctgcg ccacataacc taaggttttc atggtctgta aaaagcgata
      721 aacggtgctt tttgacatca tgacgcgctg cgacagctcg gttatcccta tttcgcgctc
      781 ttcacccagc gcctgcaaaa tgccaaaaac ttttagcacg gaagatacag aatcaggctg
      841 tttatccaga tctgcgttag ccatttatca cctcattgcg agtgttttat aaaaatcaga
      901 actgtttttt attataattt cgcaccaggg gggtcgcaat ccatcttttg ccggttagtt
      961 acaattctgc gacatccacc gtgaatatca gtgctagaat catacccctg ttgattattc
     1021 accaaagata taaaattcct atgccaaaag ttcaggccga cggcctgcca ttgccccagc
     1081 gatacggtgc gatattaacc attgtgattg gtatttcgat ggctgtcctt gacggcgcaa
     1141 tcgccaacgt cgccctgcca acaatcgcca cggaccttca tgccacgcca gccagttcca
     1201 tctgggtagt gaacgcctat caaatcgcca ttgtcatctc cctgctctcg ttttcgtttc
     1261 tgggcgatat gtttggctat cgacgtattt ataaatgcgg tctggtcgtt tttctgttgt
     1321 cttcactgtt ctgcgccctt tctgattcgc tgcaaatgct cacccttgcg cgtgtcatac
     1381 aaggtttcgg cggtgcagcg ttgatgagcg ttaataccgc acttatccgc ctgatctatc
     1441 cacaacgttt tctggggaga gggatgggca taaactcgtt tattgttgcc gtctcttctg
     1501 ctgccgggcc gacaattgct gcagcaatcc tctccatcgc atcctggaaa tggttatttt
     1561 taatcaacgt accgttaggt attatcgccc tgcttctggc gatgcgtttt ctgccaccca
     1621 atggttctcg cgccagtaaa ccccgtttcg acctgcccag cgccgtgatg aacgcgttaa
     1681 ccttcggcct gcttatcact gcgttgagtg gtttcgctca ggggcaatcg ctgacgttaa
     1741 ttgctgcgga actggtggta atggttgttg ttggtatttt ctttattcgc cgccagcttt
     1801 ctcttcccgt accgctgcta ccggtggatt tactgcgtat cccgctgttt tcactttcta
     1861 tttgcacatc tgtttgctct ttctgcgcac aaatgctggc aatggtttcc ctgccctttt
     1921 acctgcaaac cgtgctcggg cgtagtgaag tcgaaacagg tttacttctg acaccgtggc
     1981 cgttagcaac gatggtgatg gctccgttgg caggctattt gattgaacgc gtacatgcag
     2041 gattgctggg tgctctaggg ttgttcatca tggctgcggg gcttttttcc ctggttctgc
     2101 tgcccgcgtc acctgcggat atcaatatta tctggccgat gatcttatgt ggcgctggat
     2161 ttggcttgtt ccagtcaccc aataaccaca ccattattac ctccgctcct cgcgaacgta
     2221 gcggtggagc cagtggcatg ttaggaacgg ctcgtctact gggtcagagt agcggcgcgg
     2281 cactggtggc gctgatgcta aatcagttcg gtgataatgg tacgcacgtt tcgctgatgg
     2341 ctgcggctat tctggcagtg attgctgcct gtgtcagtgg tttacgtatc actcagccac
     2401 gatccagggc ataaaaaaag cgcgtcgatc agga
//
LOCUS       DAJTSL010000279         2434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTSL010000279 DAJTSL010000000
VERSION     DAJTSL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.282"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1036)
                     /locus_tag="OO433_004097"
     CDS             complement(<1..1036)
                     /locus_tag="OO433_004097"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6052359.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKAS"
     gene            1168..2277
                     /gene="apbC"
                     /locus_tag="OO433_004098"
     CDS             1168..2277
                     /gene="apbC"
                     /locus_tag="OO433_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6052360.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHCLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
BASE COUNT          592 a          603 c          650 g          589 t
ORIGIN      
        1 tgctggcttt aataaaggtg ccgcgagact tggacatctt cgcgccgttc accgtcacat
       61 agccatgaac aaacaggttg gtcggcttgc ggaagttgct gccttccagc atggcaggcc
      121 agaacaggct gtggaagtaa acgatatctt taccgatgaa gtggtacagc tcggcggtgg
      181 agtctttctt ccagtattca tcgaagctta cgctgtcgcc gcgcttgtcg cacagattct
      241 tgaaagaacc catgtagcca atcggtgcgt ccagccagac gtagaaatat ttgcccggcg
      301 cgttcggaat ttcaaaaccg aagtaaggtg cgtcgcggga gatatcccac tgttgcaggc
      361 cagattcaaa ccactcctgc attttgtttg ccacctgctc ctgcaacgca ccgctgcggg
      421 tccatgcctg caacatttcg ctgaaagagg gcagatcaaa gaagaagtgt tcagaatcac
      481 gcattaccgg ggtagcgcca gaaaccaccg atttcggctc gatcagttca gtcgggctgt
      541 aggtcgcgcc gcagacttcg cagttatcgc cgtattgatc cggggattta catttcgggc
      601 aggtgccttt cacaaaacgg tccggcagga acatgccttt ttccggatcg tacagctgag
      661 agatggtgcg gtttttaata aaaccgtttt ctttcaggcg agagtagata agttctgaca
      721 actggcggtt ctcttcgctg tgtgtcgagt gatagttgtc atagctgatg ttaaagcctg
      781 cgaaatcagt ctgatgctcc tgactcattt cgccaatcat ctgctccggg gtgataccaa
      841 gctgctgagc tttcagcatg atcggtgtac cgtgggcatc gtcggcgcag atgaagttga
      901 cctcgtggcc gcgcattcgc tggtaacgga cccagacatc agcctggatg tgctccagca
      961 tatggccgag gtggattgag ccgttagcgt acggcagtgc gcacgtcacc agaattttct
     1021 tcgcgacttg agtcatagta ggcattactt cttaatagtg aaaaggggtt agatagtacc
     1081 aaatgggaaa atgttaagta agtacttctc tatattcttc aggtaaacta tcagctgatg
     1141 gtttatctat acaaaaaagg agtcgggatg aacgaacaat cccaggccaa atcaccagaa
     1201 gccttgcgcg caatggtcgc cgggacactg gccaattttc agcacccaac cctgaagcat
     1261 aacctcacca cgctgaaagc gttgcaccat gtcgcctgga tggacgacac gttgcatgtg
     1321 gaactggtta tgccattcgt ctggcatagc gcgtttgaag agttaaaaga gcaatgtagc
     1381 gccgaactgc tgcgtatcac cggggcgaaa gctatcgact ggaaactgtc gcacaatatc
     1441 gccaccttaa aacgcgtgaa gaatcagccg ggtattaatg gtgtgaagaa catcattgcg
     1501 gtcagttccg gtaaaggcgg cgtgggtaaa tcctccacgg cggtaaacct ggcgctggca
     1561 ctggcggcgg aaggggcgaa agtcggcatt ctggacgccg atatctacgg cccatcaatt
     1621 cccaccatgc tgggcgcaga aaatcagcgt ccaacttcgc cggacggtac ccatatggca
     1681 ccgattatgt ctcattgcct ggcaaccaat tcaattggtt atctggtcac tgacgacaat
     1741 gcgatggtgt ggcgtggacc gatggcgagc aaggcgttaa tgcagatgtt gcaggaaacc
     1801 ttgtggccgg atctcgacta tctggtgctt gatatgccgc caggcaccgg tgacattcaa
     1861 ttgacgctgg cgcaaaacat tccagtaact ggtgcagtcg tggtcaccac cccgcaggac
     1921 atcgcgttga tcgatgcgaa aaaaggcatt gtgatgttcg agaaagtcga ggttccggtg
     1981 cttggtattg tcgagaacat gagtgtgcat atttgcagta actgtggtca ccatgaaccg
     2041 attttcggta ccggtggtgc agagaaactg gctgaaaaat accacacaca gctgctgggg
     2101 cagatgccgt tacacatctc cttacgtgag gatctcgata aaggcacgcc aacggttatc
     2161 agccgtccgg agagcgaatt taccgctatc taccgccagc tggctgaccg cgttgcagct
     2221 cagctctact ggcagggtga agtcattcca ggcgagattt ccttccgcgc ggtctaacgc
     2281 ctattaacca ttctggttat tcttcataca ccgcagagat gttacattga tgcggtgtat
     2341 tagtttttgc cgcattaatg aacacttatt taatactgcg aatattctgc ggaccttcgt
     2401 ccgaaactct acaaagtcga ttgatcatac aggc
//
LOCUS       DAJTSL010000280         2382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTSL010000280 DAJTSL010000000
VERSION     DAJTSL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.283"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="OO433_004099"
     CDS             complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="OO433_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6052361.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     H"
     gene            complement(1050..1355)
                     /gene="ccdB"
                     /locus_tag="OO433_004100"
     CDS             complement(1050..1355)
                     /gene="ccdB"
                     /locus_tag="OO433_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6052362.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1357..1575)
                     /gene="ccdA"
                     /locus_tag="OO433_004101"
     CDS             complement(1357..1575)
                     /gene="ccdA"
                     /locus_tag="OO433_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6052363.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2260..>2382)
                     /locus_tag="OO433_004102"
     CDS             complement(2260..>2382)
                     /locus_tag="OO433_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6052364.1"
                     /translation="TDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC"
BASE COUNT          641 a          509 c          550 g          682 t
ORIGIN      
        1 atgggatgat aatgggttat cactaatatg tattgaacat tcattcctca gattaagaat
       61 gctttccggg atatgactta tctgatttct atcaaaaaaa tactctctga caacgggatt
      121 atttccctca gaaaatgctg gtagctcagt gaggaaatta cgggtcgctc tgagattttt
      181 aagagcctgt gggagcgatg gtaaggagac tatttcgtta tagctgatat tcatagtcaa
      241 taacgaatcg ggtaaagctg gcaggttttc cagctcattg tgttgtacct gaagtgactc
      301 cagcgcagga ggtagcacgg gcaattctga taatttattg ctggcggaat ataatttttt
      361 tagcgtaaca ggcagttcag gtaggttagt taattgatta tatgatacat tgagcagcgt
      421 tatctgagct ggtaagttgt caggtagcga ggacagattt aaacggtcca gtcgaagttc
      481 atcggaatta ttgataagac attctttaag tcgggagaca gcctcatcac gctcttcacc
      541 ggggagcgct tgtttttccc atttatccca tgctgaaaag taatcagcat atgtaccgga
      601 aatagtgtta taaaaagaat tttggggcaa tgaaaagtta ttatttatcg gtaacatgat
      661 gggcatccag ttaaagccgg tggacaaatg gctttgttaa aagaaataag caggagtttt
      721 tatgaagttc attccatgaa atgaacttca gcactttttt attataataa ggataaacaa
      781 aattggctgt aaaaaaacga tactgtttca gttaaccgtg aatggcctgt ttgcaccgta
      841 ttgttcttgt ttttcggtta gttaaacatc gtacttcata tttgaacggt aagcggctcg
      901 tcagaaccgt attgatattt actgagaggt cagatcaact ttccagggca acagatcgcg
      961 tacccagttt gccggccagt cttggatatg ctcaatgacg taacgcagcc attttgtcaa
     1021 cgccacgatt tttgacccta gcctgacatt tatattcccc agaacatcag gttaatggcg
     1081 tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat aacgaagatc
     1141 ggcacactgg ccatatcagt ggtcatcatg cgccagcttt catccccgac atgcaccacc
     1201 gggtaaagct cacgggagac tttatctgac agcaggcggg cactggccag ggggatcacc
     1261 atccgtcgtc cgggcgtgtc aataatgtca ctctgtacat ccacaaacag acgataacga
     1321 ctctctcttt tataggcgta aaccttaaac tgcatttcac cagtccctgt tctcatcggc
     1381 aaaagagccg ttcatttcaa taaaacgggc gatctcagcc atcccctgct gattttcagc
     1441 ctgccagcgt tccgcacgca gacggcgggc ctctttttgc atggccgtat ttaccaggcc
     1501 tgagatattc acgtttgcgg atttaagtaa ctgatagctg tcactgtcta tagtgacagt
     1561 aatacgctgc ttcatgataa gccctcaaaa acatacttat gatatgtata tgagtatata
     1621 ttaatggtac aaaccatcaa tatccataaa aagtacacca ttaattcact ataagttttt
     1681 aatacaattt tttctgctgc gattttttta tacgataatt agcatactta taacaccgta
     1741 agtaaattac atgaaagcag gcacagcgtg atccgatttt tctgcgtata acgattttcc
     1801 ggaagtgtaa tacggttgcg ccccgggccg ggcacccttt tctgttgttg tgtacgggcc
     1861 cacagagaat ggtaccggag gcgggctcag aagtactgag tcgggtgatc gcgtcaggcc
     1921 gcgaagtact gacacgggta ttggtgactg gtgatgaagt actgccccgg gtatgatttc
     1981 aggctgattc aggttctgat tctctgtcag tccgcgtggt tgctggcctg gctgatttcc
     2041 tgttgtcagg cgttcaacaa ccgactgcag gttatagccc ggttcacgaa ccacggcagc
     2101 ctggcggctg caaatcgggg tgtggcagaa tatgagcttt cagagtaacc atccagaatg
     2161 agttcccatg tcacacaacc tggagcacca gaaagtccac accagaatgg tgaaagaggt
     2221 tctgaaagcc gtcgccagag ccaataatca ctgaagtggt caacaaaaac tggccaccga
     2281 gttagagttt ttccagtatc gattttccga ttcgtttggg ggtaacccac cattatattc
     2341 gtgcggtctt agtgcgctgt aatatccaac gatatagtcc gt
//
LOCUS       DAJTSL010000281         2354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTSL010000281 DAJTSL010000000
VERSION     DAJTSL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.284"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..758
                     /locus_tag="OO433_004103"
     CDS             <1..758
                     /locus_tag="OO433_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR6052365.1"
                     /translation="NQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDD
                     LMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKY
                     IPASLCRTNASGTPVNGYFLTLVQVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRY
                     LWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTA
                     EWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(759..1667)
                     /locus_tag="OO433_004104"
     CDS             complement(759..1667)
                     /locus_tag="OO433_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6052366.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINDARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQISQSQRHRPQ"
     gene            1767..>2354
                     /locus_tag="OO433_004105"
     CDS             1767..>2354
                     /locus_tag="OO433_004105"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HCR6052367.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT          621 a          497 c          575 g          661 t
ORIGIN      
        1 ttaatcaaac gcgcaaccca ggaaaagaat ttccaaaagg gatgttatgc ctggcggtga
       61 tggttgcggt ttgtgccatt ctgggctcac tggcgatggg gatgatgttt gactcgcgta
      121 atatcccgga tgacttaatg actaacggtc aatattacgc ctttcagaag ctgggcgagt
      181 attacaacat gggtaatact ttaatggtga tttacgccat tgcgaatacc ctgggacaag
      241 tagctgcgtt ggtattctcg attgatgccc cgcttaaagt gctattaggt gatgctgaca
      301 gcaaatatat tccagccagt ttatgtcgta ccaacgcttc tggtacgccc gttaatggct
      361 attttctgac cctggtgcag gtggcaattc tgattatgct gccgactctc ggcatcggtg
      421 atatgaacaa tctctataaa tggctgttga atcttaactc ggtagtgatg ccgctgcgtt
      481 atttatgggt atttgttgca tttattgcag tcgttcgcct ggcgcagaaa tataaaccag
      541 aatatgtctt tattcgtaat aagcctctgg caatgaccgt cgggatttgg tgttttgcct
      601 ttaccgcctt tgcctgtttg acggggatct tcccgaaaat ggaagccttc actgcagagt
      661 ggaccttcca gttggcgctg aatgttgcaa cgccgtttgt gctggtagga ttaggactga
      721 ttttcccgct gctggcgcgt aaagcgaata gtaaataatt attgtggtcg gtgacgctga
      781 ctctggctga tctgacccgt ttttacataa tcgagaaatg cgcgcaagcc gcaggacata
      841 tactggcggt ttggataata gatctggaag cctggacgct ctgtgctcca gtctcccagt
      901 acacattcta aacgaccagt atcaaggtac tccttgatct gttcatagag cagatacccg
      961 ataccagcac ccatcaaaac ggcctccagt tcagcatcaa catcatccag aatgatattt
     1021 cctgcgacag caatttccag ctcctttgca aattgccagt ggaaaggttt gccgctggga
     1081 taacgaaaaa caacacattg atgattaagc agatcatgag gatggcgggg tttgccatat
     1141 cttgcaaagt actctggcgt tgctgctaca cataatttca ctggcgggcc gatcgccacg
     1201 ctgatcatat ctttttcgac gatgccacta agtcgaacgc cagcgtcaaa gccctgttgg
     1261 acgatatcga caagagagtc gtcagttgtg agctcgactt taatatcagg atattcacgt
     1321 gtgaaaccaa ccagtaaagg cattaaaaat atacgtgcag ctacccgggc gtcattgatt
     1381 ttgagcgttc ctgtcggtgt cagacgaaaa tcattcatct catctaacat gatctgaatt
     1441 tcgtcgaaag ctgggcgcaa tcgttcatag agattagaac ccgcttccgt taacgatacg
     1501 cttcgggttg tccgattgaa taagcgaatt ttaagacgtt gttccagtgt tttaatacta
     1561 tggctaatgg cggacgagga taagcctaac tcatcgcccg ctgcacgaaa gctttgatta
     1621 cgggccacgg caaagaaagt ggcaaagtca gacatattca tccgcattag tgaaaatcct
     1681 tcattaactc atcctgaatc atcacgttta caggattatg ccgcctgcgt aaagtgcgct
     1741 ccagaactta acgtggaggt aaaattatgc agtctgaacg tatttatttg gtatgggccc
     1801 atcctcgtca tgattcattg accgcacata ttgctgatgc gatccatcag cgggcaatgg
     1861 agcggaaaat acaggtgacg gaactcgatt tatatcggcg taatttcaac ccagtgatga
     1921 cgccggaaga tgaaccagac tggaagaata tggataaacg ttattctcca gaggttcatc
     1981 agctttattc agagctgctt gaacatgaca cgttagtggt ggtttttcct ctctggtggt
     2041 acagcttccc ggcaatgcta aaaggatata ttgacagagt atggaataat gggctggctt
     2101 atggagatgg gcacaaatta ccattcaata aagttcgttg ggtggcgctg gttggaggag
     2161 acaaagaatc atttgtccag atgggctggg aaaaaaatat aagcgattat ttgaaaaata
     2221 tgtgcagtta tcttggtatt gaagatgccg atgtcacttt cttgtgtaat acagtggtat
     2281 tcgatgggga agaacttcat gcgagctatt atcagtcgtt attatctcag gtacgggata
     2341 tggtagatgc acta
//
LOCUS       DAJTSL010000282         2237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTSL010000282 DAJTSL010000000
VERSION     DAJTSL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.285"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(152..571)
                     /locus_tag="OO433_004106"
     CDS             complement(152..571)
                     /locus_tag="OO433_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6052368.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            651..905
                     /locus_tag="OO433_004107"
     CDS             651..905
                     /locus_tag="OO433_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6052369.1"
                     /translation="MNQKTLEDVIKTVRVAVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            902..1327
                     /locus_tag="OO433_004108"
     CDS             902..1327
                     /locus_tag="OO433_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6052370.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDALKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            1399..>2237
                     /locus_tag="OO433_004109"
     CDS             1399..>2237
                     /locus_tag="OO433_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCR6052371.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRYVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQAMTASPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLR"
BASE COUNT          612 a          487 c          583 g          555 t
ORIGIN      
        1 gcattctgtg gggcggggtg ggtggcaggt atataatgta ctttgcgttc attgttgtaa
       61 agtgcttatg gtacattttg tgtgtaaaaa aatgagatgg gataaagtga agcacaaacc
      121 cggaggaagg cgctaccgga tttatgctgg tttaagaggc tttttgtttt ttctttcgtg
      181 ctaactcttc gtaaattgca ttgtacttct gttttttctc ttcaagagtt tttaaaagtt
      241 catctgtctc actgtcaggg agctcgtcca gaaggtcaat gatgattttt tgtcttggat
      301 ttaactcctg atagaaacgt acctgtccac tttcttctgt atcctctccc aaaagatagg
      361 ttggtgttgt tcctattagt gttgctaatt cccttaattt ctcccggcga ggaattgttt
      421 cgccattaaa ccatttgcta accgcttttg gtgttaattt cattcgacgg gcaatttctg
      481 cctgccttcc atgttgttca taaccagcgt tttcacaggc tagcgcaagc ctactggcga
      541 actctttacg cgctttatct tcatgaacca taagttcaat gatattcgct cttgaatgta
      601 ctgtcagttc tgttatagca tgtactcaaa gttcacattg tgagggtgat atgaaccaga
      661 aaacacttga agatgtaatc aaaactgttc gcgttgctgt tgtggccgac gtttgtggtg
      721 tcagccaaag agcaatctat aaatggatgg ataacggaaa attgcctcgc acagaatata
      781 ccggcgaaac aaattacgct gaaaaaatcg ctcttgcatc aaacggatta ttttctgccg
      841 atgcaatttt aactattggc aggaataaaa ctactacgaa aaagctgatg ggagttgatt
      901 catgaaaatc aagcatgaac acatccgcat ggcgatgaat gcctgggcgc atccggacgg
      961 cgaaaaagta ccggctgcga aaattaccaa agcgtatttc gagctgggaa tgacgttccc
     1021 ggaactgtat gacgacagcc atccggaagc cctggctcgc aatactcaga aaattttccg
     1081 ctgggtggag aaagacaccc ctgatgcgct taaaaaaatt caggcgttgt taccagctat
     1141 cgaaaaagca atgccacctc tgctggtggc ccgaatgcgc agccatagct cagcctattt
     1201 tcgggaacta gtggagacgc gggaacgact ggtgagagac gctgatgatt ttgtcgcagt
     1261 ggcgatcgct ggtttcaacc agatgaatcg tggtggccct gcaggaaata ttgtggctgt
     1321 gcattgactc gcaatattca taccggatca cttccggcaa tttgtgagta aaaagattcg
     1381 gtatcaaaag aggtgagtat ggctaacgcc tggctcagat tatggcatga catgccaaat
     1441 gaccctaagt ggcgaacaat tgccagggtg tcagggcagc caattgcaac agtgatggca
     1501 gtgtatatcc acctcctggt gagcgcgtca cgaaatgtca cgcgaggtca cattgatgtc
     1561 acgacagaag atttggcaag tgcgctcgac gtgacagaag aggtaattga ttcaattttg
     1621 cagacgatgc aggggcgggt acttgatggt gatttaatca ctggatggga aaaacgccag
     1681 gtgctgaaag aggacaacgg caatatttcg caaaccgcaa aatctcctgc agagcgcaag
     1741 agggcgcagc gagagaggga aagaaagcgg gaacaaaatg gcgattgtca cggcgcgtca
     1801 cgatatgtca cgcacatgtc acgacgagtc acgacagata aagatacaga taaagataca
     1861 gatcaagaag atcaaaacac tatggtccat ggcgtaaaaa acgccacgaa ccaggcaggg
     1921 gatgttcaga ccgtcaatcc tggtcagcca gcaggcacga caccggaagc cgattcagcg
     1981 tatgcgctga aagccgattc gggcgctgtg cagcaggcga tgaccgcaag tccggagcaa
     2041 tcacaccaac tgcagcagcc tgaagccgat tccgccattc agcgggaagc cgatcgggta
     2101 gtcccggaaa acaccgggca gtctgtggga cgagtggatt atccggatgt gttcgaacag
     2161 gtctggcggg agtacccgtt gcgtgccggg gcaaacccga agaaatccgc tttcagtgcc
     2221 tggaaggcca gattacg
//
LOCUS       DAJTSL010000283         2234 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTSL010000283 DAJTSL010000000
VERSION     DAJTSL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.286"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(25..>2234)
                     /gene="pdeF"
                     /locus_tag="OO433_004110"
     CDS             complement(25..>2234)
                     /gene="pdeF"
                     /locus_tag="OO433_004110"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6052372.1"
                     /translation="KLNATYIKIRDKWWGLPLFLPFLILPIFAHINTFAHISSGEVFL
                     FYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIIIIIIII
                     LCWGGYRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENL
                     VGVMPFNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTK
                     KEFALWLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWA
                     VVLMISIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRL
                     AYVDPVVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWL
                     SPLLEPGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSY
                     CYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQA
                     LEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWV
                     IEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPFEVSQLLAKYQIEAWQLIFEVTE
                     SNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIV
                     SNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKP"
BASE COUNT          673 a          530 c          499 g          532 t
ORIGIN      
        1 ttcattcaac gtatcaatta acggctacgg cttaccaata agataaccct gcatataatc
       61 gatccccaaa gagagcaccg cctcgcggat ctcttcgttt tcaacgtact ctgccactac
      121 ccgcattttc ttcattcgcg ccaggtggca aatcgatgcc actatctgat aatccagact
      181 atttgacaca atattgcgga taaaactgcc gtcaatttta agcagatcgg cattcacatt
      241 tttaagccgt gcatagctgg cgtagccggt gccgaaatca tcaatcgcaa tctggcagcc
      301 taattcctga agatgctgca aggtgatttg cgcctgctta acattggtca gagcattact
      361 ttcggtgact tcaaaaataa gttgccacgc ttcaatctga tatttagcca gcaactgact
      421 gacttcaaaa ggaaaacgag cctgacatac cgaggttgga gacagattaa tagcaaaacg
      481 gtgagcgggc atcttcgctc tgttttcagc cataaattgc agcgtatgct caatgaccca
      541 catgtcgata ctcgacgata aaccaaattc gtgcgcgacc ggcaagaagc tatcggggct
      601 gatcagttca tcattctcac ctttcatgcg cagaagaatt tcatggtaaa catcaccacg
      661 cataccggta atcggctggg ccatcaggaa aaaatggttg tgttccagcg cctgctgtag
      721 tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc aaatacattg ccccgcgacg
      781 ctgcatattt tccggggcgt tggtcacgat ggaaagttcg gcgaccgtat ttagctctcc
      841 cagcagcagg tagatatgat tcactggcga gcgcacatag cagtaactga cgccaatctg
      901 cggttgcatc ggcatgccat cccaaaagaa acgaaattgc ttgagatggc tatccagtgc
      961 ggtaatgcgc tcctggtgcg attctgtatt cagtcgcagc gcgagatcgt tacccgaaag
     1021 ctgataaaca tcttcacccg gttccagcaa gggtgacagc cagtgagaaa gtttttgctt
     1081 gtattgaatc cgcagcatga tgccatagtt cttaaccagc atttccatgc cagggatgcg
     1141 taaataacaa agcgcagacc agggggcatc acgcaacgcg cgattcaggg cgcgaacatt
     1201 tggcagatga accaccgggt ccacatacgc aagccgctga atgcgtctga ctaccgctct
     1261 ctgacgggtt gccagtacag ccatataatt gacaataaaa gagaatacca gataactgga
     1321 ggaggttatg gtcagctgcg tggtatagcc aggataaatg ggaatgtaat tttgatagct
     1381 gtggatgctg atcatcaaca cgaccgccca gagcagagaa atcagcttat aaccatagcg
     1441 catcgctccc cacatcatca ggggcagcaa taatgacaag gtataattgg tgctaaaaat
     1501 tgtgcttttt tcatttaacg gcatgcataa cagcaacaat aaagcaccta atgccagtag
     1561 ccagagcgcg aactcttttt tggtgacttt ggcatcaacc tgctgtttta attgcgaata
     1621 atagctacgt aaataaaatg gatttcgcac tacccgaatg atgaagtagc acagcgggac
     1681 accgatcaga ttacccacca gtaaggcctg ataattgatt aaagtcccga ggttaaaagg
     1741 catgacaccg accagatttt ctctgctcgc caataatcct acaaatgcag caaactggaa
     1801 aagtatcaga aacagcgttg caggaaacac aatctgccag aatatacgct gggaaattaa
     1861 acgggtatca ccatgtgaaa cgttgttacg ccggggagta aagaccctgt aaccgcccca
     1921 acagagaatg ataatgataa tgataatgat aatgataata aaattagccg ttaatgatag
     1981 cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa atccctaagg cgatccctgg
     2041 caatgccgcc cagctgaaaa acatcatcat gctgatcatc agtgccagag gcagataaaa
     2101 gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta atgtgggcga aaatgggcaa
     2161 gattaaaaaa ggcaggaaca gcggaagccc ccaccattta tcacgtattt ttatataagt
     2221 tgcattcagt ttca
//
LOCUS       DAJTSL010000284         2214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTSL010000284 DAJTSL010000000
VERSION     DAJTSL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.287"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            62..383
                     /locus_tag="OO433_004111"
                     /pseudo
     CDS             62..383
                     /locus_tag="OO433_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(417..623)
                     /locus_tag="OO433_004112"
     CDS             complement(417..623)
                     /locus_tag="OO433_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052373.1"
                     /translation="MTVSYKITYEHRDGDQIRMEEVLFHSEHEPTKDEIHDAVRKDTV
                     RFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(1121..1194)
                     /locus_tag="OO433_004113"
     tRNA            complement(1121..1194)
                     /locus_tag="OO433_004113"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1160..1162),aa:Gly,seq:ccc)
     gene            complement(1273..2028)
                     /locus_tag="OO433_004114"
     CDS             complement(1273..2028)
                     /locus_tag="OO433_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6052374.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKTGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
BASE COUNT          586 a          524 c          484 g          620 t
ORIGIN      
        1 atgcattaat ctaaaaacgc tttaaatcaa tacataacca ttatctcttg ggattattta
       61 catgcgcaaa agtttaatgc tgttacttgc ttcaacattg attatttctg gatgtgcaac
      121 aaaaaaacaa ctcgttccaa ctggtggaag caaatcagac ggcacagtta ggatgagtta
      181 ttcttacggt atgtttgaaa gtcctgttgt agatccacaa caaggcatga atgcagccaa
      241 agcgcgttgt gccgcttagg ggatattcag gagccgagcc ttttggtgga ttcacttctc
      301 aatgttcaca accttcttca tctggttgta tgcaaacact tgtgactatc gaatatcaat
      361 gcactggcga tattaagaag taataattag gggcttccag ccccttctat aaccattcat
      421 ggaacaggga caaccgaaat cactttaaac cctgaaacag ccgttactcc attagatgga
      481 gaattaaatc tcacagtatc ttttcttact gcatcatgaa tttcatcttt tgttggttca
      541 tgctctgaat ggaataaaac ctcttccatt ctaatttgat caccgtctct atgctcataa
      601 gtaatcttgt agctaacagt catatcaacc tcatgcaggg gaattgtgag attattatag
      661 acaatagaaa ccataaagcc ccgttggtga tacagcaaca agcgggagcg tagcgacttc
      721 atcagattgt cagaaagatc acaccttatc aggataaccg tgtacccgtt gggagcgtgg
      781 ctgaaacgct gatagagctt gcatatggtt tgcaaggacc aatatcacca taaacctgtt
      841 tacgcagcca caaggccgca catcgggctt aaatgcgtgt gttcagtctg tcgatgacat
      901 tacaatgcca ggatgggctt tatcttcacg ttgtgctatc gcggtgtgag aggtgcaact
      961 gccgccaacg ttaggttata ctgtcatctc accgtgtaac ttaacgtgac agttactaag
     1021 ggcggatcac aatggtagaa ctcctaagca aattaaatag gtaactgttt aagttgctga
     1081 cagagaagac ttttttagtc ttctccacct ggggagattc tggagcgggc gaagggaatc
     1141 gaaccctcgt atagagcttg ggaagctctc gttctaccat tgaactacgc ccgcttcgag
     1201 atgcgtaagg cattataaac cttacgctct ccttagcaag tgccgcgctg ctgactgctg
     1261 attaattcgc catcagcatt ttggcttgct gccctgcggc ggcaagtaac gtagcggatc
     1321 aattgccgtt gcacggtaac gaatctggaa atgcaggcga acagatgccg catccgtgct
     1381 ccccatagtg gcgatttttt gcccagtctt cacgctttgc ccattattta ccagcatcgt
     1441 gtcattatgg gcgtaagccg taatgtaatc ttcactgtgt ttaatcatga tgagattacc
     1501 gtagccacgc agctggttgc ccacatacac cacctttcct gcacccgcgg cgtaaatagg
     1561 tgtaccccgt ggagctgaga tatcaatccc tttattgccg ccatctgctg tcgaatacgg
     1621 catgataact ttccctgtcg ttggccataa ccaacaacgt tgccctactg gcggccagga
     1681 agatttcggt accgctgatg acggtgtaac cgatgcggtt ttggtcgttg atttggcggt
     1741 tgatttacgt gtactactgc tacttttcgc cccacccagt tttagtttct gaccaacttc
     1801 aatggtgtaa gggggggaaa tgccgttcag tcgcgccagt tcttttacgc tggttcccgt
     1861 ggtgcgcgaa atacgatata gcgtatcccc ccgtttcacg gtgtaaacgg agccggaata
     1921 tgttcctgta tcggatgatt tgctacccga acagcccgcc aaaagcagtc caaccgataa
     1981 caacatcacg atacccagag attttttatt caggcgtccc gcactcaaaa tctttcctcg
     2041 cttaaaaaac aagacgcctt atcatagcag ccagcctctt gatacccaat ctcttgcttc
     2101 tggaaaaacg tggtaataga gaaagaggtt aatttcattt ggcaaaggga agtatcatca
     2161 gcaaaatatt cgctgtagag gtatcgaact tcctgacttt ttaccctatt tccc
//
LOCUS       DAJTSL010000285         2195 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTSL010000285 DAJTSL010000000
VERSION     DAJTSL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.288"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..139)
                     /locus_tag="OO433_004115"
     CDS             complement(<1..139)
                     /locus_tag="OO433_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 family protein"
                     /protein_id="HCR6052375.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGKNKRLHQ
                     RR"
     gene            complement(139..738)
                     /locus_tag="OO433_004116"
     CDS             complement(139..738)
                     /locus_tag="OO433_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6052376.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPIGGAITPRERELVSGFVEYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSISFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(777..1064)
                     /locus_tag="OO433_004117"
     CDS             complement(777..1064)
                     /locus_tag="OO433_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052377.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(1314..1469)
                     /gene="hokD"
                     /locus_tag="OO433_004118"
     CDS             complement(1314..1469)
                     /gene="hokD"
                     /locus_tag="OO433_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6052378.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1725..>2195)
                     /locus_tag="OO433_004119"
     CDS             complement(1725..>2195)
                     /locus_tag="OO433_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6052379.1"
                     /translation="NIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGIRCTMCFD
                     MRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWDYNWRKQG
                     GSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKEEKE"
BASE COUNT          546 a          588 c          492 g          569 t
ORIGIN      
        1 ttcttctttg gtgtaatcgc ttgtttttac ccgccagtcg gtaatgcgcc agcacagacg
       61 tttccggatc gtgattgcag tagccaggaa ttctgacggt gcacatctgc ccccgcgccg
      121 ctttacgtaa atccaccatt acgcaaactc cagtagctgc gcggccacat tttcgacttg
      181 ttccggagag gaaaatttac ggaacaggat ccagttccac agtacattca gcacagattt
      241 ataaacctgc tgaaactcgg tttcgtccat attcgcaaac gagatggatt tcgccctgcg
      301 cccacggcta ccgtccggat aaatatgctc ggtgtaaaat ccggcctgaa tggttaccca
      361 ctcgcggaaa gcctcaaacg actttagcaa tgctgtatct cgggttctgc gtgtcgcaac
      421 tgtattcaga tattgctctg cggcttcgct cagagctggc gtatgttccc ggcctactga
      481 ttcgcacagg tactcaacga agcctgatac cagttctcgt tcgcgaggcg taatcgcccc
      541 accgatcgga gtccagtaat cgaatcccag ttgcaggagt ttgaaaaaac gcttgtggaa
      601 tgcgtagtta cgcacacgct taaagtctgc gtgtatccac tcacctgttt tgatttgatg
      661 cagaaaatcg caactctccg gcgtcgccgg gagaagtaat ccggaagagg tttgtttgat
      721 cagttgtata tgcgccatcg gctttctccg gtggcacggt gttacacagc aggagttcaa
      781 tcctgctcaa gattgtagat gagtttattc ttcagcaaaa gcagaaaacc agccttaaaa
      841 ccaatctctt tcaaaacccg taatgatgtg acaaattcgt cctcacgcaa aataaaaccg
      901 tccgtcagaa gtccattaca aaaataaaat aacacagcac cgctcttcct ttgttgagat
      961 tgcaaacatc taatgcggca atggctgaca atcgctccat tctcaacgcg cacagcatag
     1021 aggccatttt cactaaaaat ttcacgcaat tcttcgattt tcatcttcag aatccttcca
     1081 gataaatagc tctcccctgt tcggggtcca tccctcttct ccctgcgcgc tacttaagtg
     1141 agtcgattct atctgcgaag gtgcgcgaat caaattcacc ggaaataaac aacaaaaaac
     1201 ccgccgaaac gctgagagat cacagcgatg ggcgggttaa gtgcgggtgc gttgaggatg
     1261 cctgacacat cagaggtggg cggggatggg atcagctccc cgcccggtca ctcttacttc
     1321 ctggattcgt agtctacgaa gacagcgacc tccgtctggc cggttcggat tcgtacctca
     1381 cagaggtctt tcctcgttac cagtgccgtc actatgacgg ttaaacagat gacgatcagg
     1441 gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt cggcatgtaa
     1501 gaggctaacc tacatgtgca aagcatgaaa ttggcctcag attaatgtta agcgtcttgc
     1561 cggacgcgta atgttaactg gggcttttct ctatctgcct tttggtgttc atgcctgaag
     1621 cagatagcct gaagcagata gcctgaagca gatagcctca agcacccgca gtcattctac
     1681 ttacctcgcc aatatgaaat caatcagaaa ggtgccccat aaaatcactc cttctcttct
     1741 ttaccgtagt ggagttgacc aattttgata agagggcgtc cctgagattt gcggtgtaga
     1801 ttggtatcgc gcagagaata cacacagcca caatattcct gctgatagaa tttttcgcgc
     1861 ttgctgattt caatcatacg ggacgagcct ccctgcttgc gccagttata atcccagtac
     1921 accatgcccg gataatgcgc gacggctcgc cgcccacagt cgttaacctg ctgcatattt
     1981 ttccagcgtg aaatgcccag tgaactgctg atcacactga aaccattttc agcggcgtac
     2041 agcgctgtcc gctcaaaacg catgtcaaaa cacatggtgc aacggatccc tcgttcgggc
     2101 tcccattcca ttcctttggc tcgttcaaac cagttgtctg tgtcgtaatc agcatcaata
     2161 aacggcacgc cgtgttgttc agcaaagcga atatt
//
LOCUS       DAJTSL010000286         2178 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTSL010000286 DAJTSL010000000
VERSION     DAJTSL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.289"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            376..519
                     /locus_tag="OO433_004120"
                     /pseudo
     CDS             376..519
                     /locus_tag="OO433_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            666..934
                     /locus_tag="OO433_004121"
                     /pseudo
     CDS             666..934
                     /locus_tag="OO433_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1330..>2178
                     /locus_tag="OO433_004122"
     CDS             1330..>2178
                     /locus_tag="OO433_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6052380.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPFPLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLAMANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          730 a          463 c          366 g          619 t
ORIGIN      
        1 gcttcttacc atatttgaaa taaagctcaa tagcgcaaat tttttgttct tcagtaaaca
       61 tgtttcatct tctgagagtc caggaaatcg tccgcatccc cattaccgtc ttatatcact
      121 ggcgctgaca tgtgctcatt gatgaagtga atctgaatga catgttttgc catatcaacg
      181 ccaaccggtg tatatttcat ttgtggagtc tccagtctga ggacgcttta tgcatcccgt
      241 attgggcagt atgatgccgg aaagctgcga ggctccacac ctgccagttt caccctttta
      301 accatcatac tactccgtgt ttaattgtgt gggatgcgtt cattacattt taacagtgtc
      361 accccgtcgg ccacgaaaga taaacaggtg accagaaaag ggatcttcat ccagcacatg
      421 ctatatcttg aacgtaacaa cctgaccctc cgcacccgca tcaagcggct ggcacgtaaa
      481 acaatctgct tctcgcgttc cgtggaaatc cacgaaaagt catcggctcc ttcattgaaa
      541 aacacatatt ctactgattg gaagcgccac ccgaaaaagt atcgtttttt tacagtaaac
      601 ttcatttagc cgactataat gtaaaaaaat aaatttttta ccgaaatata acataaggaa
      661 gtaatatgct tacacaaact atattccctt gtcttcccaa aaaacaagaa aatatcatcc
      721 ctgaagtatc aaatactata ttagtatctt ctactgttac tacagatgga tgtactgttt
      781 ttaataaaca aaaacagcca tatacgaatt gcaaatacct gctaccaacc gaacgaaaaa
      841 gttgaaattc acagctacag aaatacaatg gttgacaaag ataaatgaag ccggaattga
      901 tgaaaaacaa agccaacggt attctgattt ctgaagttaa tttcagactc gtttaagctc
      961 cattttcaaa aaaaaaaaat atatcagggt ttgttactaa taacttctga aaacagaaaa
     1021 aaaatagagc agtaattcta tttcagagag gagtaactcc atctatcctc gatacgtact
     1081 gaacgttatc gtgatatatt catcatgctt tcttcagcat tattcgagta cagggagaga
     1141 cgcaatgacc ttaaatacct ttttatggct gaattaccat tctcaggaat aacagcagac
     1201 tccttcccat agacggctta gggggaaagc aatacccgga gaaagggtac aaagcaaact
     1261 gttttaaaat taataagcta tattctattt cactagaatt cttatcgctg ataagaaatt
     1321 ttagttataa tgattaaatc aaccaatata caggcaatcg gttctggtat tatgcatcaa
     1381 ataaacaata tatactcgtt aactccattt cctttaccta tggaactgac tccatcttgt
     1441 aatgaatttt atttaaaagc ctggagtgaa tgggaaaaga acggtacccc aggcgagcaa
     1501 cgcaatatcg ccttcaatag gctgaaaata tgtttacaaa atcaagaggc agaattaaat
     1561 ttatctgagt tagatttaaa aacattacca gatttaccgc ctcagataac aacactggaa
     1621 ataagaaaaa acctattaac acatctccct gatttaccac caatgcttaa ggtaatacat
     1681 gctcaattta atcaactgga aagcttacct gccttacccg agacgttaga agagcttaat
     1741 gcgggtgata acaagataaa agaattacca tttcttcctg aaaatctaac tcatttacgg
     1801 gttcataata accgattgca tattctgcca ctattgccac cggaactaaa attactggta
     1861 gtttctggaa acagattaga cagcattccc ccctttccag ataagcttga agggctggct
     1921 atggctaata attttataga acaactaccg gaattacctt ttagtatgaa cagggctgtg
     1981 ctaatgaata ataatctgac aacacttccg gaaagtgtcc tgagattagc tcagaatgcc
     2041 ttcgtaaatg ttgcaggtaa tccactgtct ggccatacca tgcgtacact acaacaaata
     2101 accaccggac cagattattc tggtcctcga atatttttct ctatgggaaa ttctgccaca
     2161 atttccgctc cagaacac
//
LOCUS       DAJTSL010000287         2160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTSL010000287 DAJTSL010000000
VERSION     DAJTSL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.290"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..303)
                     /locus_tag="OO433_004123"
     CDS             complement(<1..303)
                     /locus_tag="OO433_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="catalyzes the transfer of alpha-xylosyl residue;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6052381.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYA"
     gene            complement(313..1695)
                     /locus_tag="OO433_004124"
     CDS             complement(313..1695)
                     /locus_tag="OO433_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCR6052382.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVLGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1988..2082
                     /locus_tag="OO433_004125"
     tRNA            1988..2082
                     /locus_tag="OO433_004125"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2022..2024,aa:Sec,seq:tca)
BASE COUNT          597 a          539 c          505 g          519 t
ORIGIN      
        1 agcataacgt tctgtgtttt cgattgtgac cttcacgtcc tgcaaaatat tgagcggata
       61 atgaggaccg ttattcagcg ctccctgaaa atgctcaatc cgcacaccga caataccttc
      121 ctgtggggag aaaaagcgca gcgtaaataa aggcgtatca agctgccagg tacgttcacg
      181 cacatcacgg ggggcagcat agaccaccat ttcattatcc tgctgttcaa cctcgaacac
      241 ctgaagcggg tgaatcaaat tgaggccagg ttgaatcaac cagtttccat cgctaatttt
      301 cattgccgtt ccttagttct gcacttcttg agaggtgaat tgttgctggc aacggcgttt
      361 accctgagcc agctgttcca taacggtttt cagattgtgc gtcgtgagtg agtagtagcg
      421 tttagcgata atcgcgctca gcaaataaca gatcgccgga acaatcgtga atagcgcaat
      481 aatgatgcta atcgtggcgc tgttctgcgc tttttctgcc gcatcatatc cgccataagc
      541 cagcatccag ccgataagag cgccgccgaa ggccaacccc agtttgagca caaacagcgt
      601 gccagcaaaa ctgatcccgg tcaggcgttt accattgcac cactcgccgt agtcgacggt
      661 atcggacatc attacccact ggataggtgt caccagttga tgcaacacac caatcacgaa
      721 gatgaagaca aacatagtga tgctggcctg catgggaaca aagaacatcg cgaggctaat
      781 cactgccagc agggcgttcg tccaccagaa gatagtgact ttacatttcc agtcggtcag
      841 aggttttgcc agtgcggaac caatcaggtt acccacgcaa taagtggtga gaaaagcgac
      901 aaacacttcc ggcgtgccca aaatccatgt gacgtaatac atcatcgccc caccgagtac
      961 gcacaccgcc aggatattga aaatggttag taaaccgaca atccgccact ggtcgttttg
     1021 ccagatatca cgtaaatctt cccgcataga cgttgttgta ggtggtgctt caacgcgttc
     1081 tttagtggtg aagaaacaaa atgccagcat catgaatgcc accacggaaa ggaccgcgat
     1141 accgccctgg aaaccgagtg gtttattatc accgccaatt aaattaacca gtggcatcat
     1201 cagaacagta gaaagcatgc ctcccgcggt cgccagcaca aaacgccagg attgcagcga
     1261 gatacgctga gtcgggtcat tggtgattac accacccaat gcgcagtaag ggatattgac
     1321 gacggtataa agtaaggtaa gtagggtgta agtaattgct gcatagatca ttttgccgtt
     1381 catactgaga tctggcgtgc tataggccag tacacagacg atcccgaatg gcagtgcgcc
     1441 aaacagtacc cacggacgaa atttacccca gcgagagcgc gttcgatcgg ccaacaaccc
     1501 catgcaagga tcggaaatcg catccagtgc acgagcgacc aaaaacatgg ttccgacaaa
     1561 accggcagga atgccaaaaa tatcggtata aaagaacatc atatataaca ttacgttatc
     1621 gaaaataatg tggctggcgg cgtctcccat gccataacca attttctctt taacggacaa
     1681 cacttcactc ttcatctgct tttccttgat gctatagggg tgctgagacc gataacgcat
     1741 tttgtaaacc atccctgtga gagcgaatat tccgatatct ggttattaat ttatgattct
     1801 tgttttatgt gatcgtggta gcgttaattc cgctcatatg tcattgtaaa atatgggttt
     1861 tatatgaact ataatgcttt cgtgataata cgctgcgtgt attaggcgga aaaaactgat
     1921 ctgggggatg tagaaactca aggaagtagc tataatgcgc cccgcctcca tgtagcaatc
     1981 gaggcgcgga agatcgtcgt ctccggtgag gcggctggac ttcaaatcca gttggggccg
     2041 ccagcggtcc cggacaggtt cgactcctgt gatcttccgc caaaatgcct cttctgacgt
     2101 tctccaaccc cttgtaatac tgtcaatcca gatattttct actgctcaat attcaatcgg
//
LOCUS       DAJTSL010000288         2143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTSL010000288 DAJTSL010000000
VERSION     DAJTSL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.291"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..116
                     /locus_tag="OO433_004126"
     CDS             <1..116
                     /locus_tag="OO433_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6052383.1"
                     /translation="VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            113..463
                     /gene="tnpB"
                     /locus_tag="OO433_004127"
     CDS             113..463
                     /gene="tnpB"
                     /locus_tag="OO433_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6052384.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDMIKILWADADGLCLFTRRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            494..2086
                     /locus_tag="OO433_004128"
     CDS             494..2086
                     /locus_tag="OO433_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6052385.1"
                     /translation="MNQKYLIRIAELECQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     ARQGVELSRALLSNWVDACCQLMTPLNDALYRYVMNSRKVHTDDTPVKVLAPGRKKAK
                     TGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQGKHPEQHLSPFRGILQADAFNGYDRLF
                     SAEREGGALTEAGCWAHARRKVHDVYISTKSATAEEALKLIGELYAIEHEIRGLPVSE
                     RLAVRQMQSKPLLTSLYKLMQEKEHTLSKKCRLRDAFRYIRKHWVALCNFSDDGLAEA
                     DNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRYILSV
                     LPEWPSNRVDELLPWNVALTNK"
BASE COUNT          539 a          539 c          614 g          451 t
ORIGIN      
        1 cagttagtgc cagctcctgc aaggtggagt tccgtcacgg taacatgacg ctggaaaatc
       61 cttcaccaga gctgctcaca gtgttgatcc gtgaactgac cgggagggga cgatgatctc
      121 actcccgtca ggcacccgca tctggctcgt tgctgggata accgatatgc gtaagtcttt
      181 caacgggctg ggtgaacagg tacagcatgt gctgaatgat aatcccttct ccggtcacct
      241 gttcatcttc cgtggccgac ggggtgacat gattaaaatc ctgtgggctg atgctgatgg
      301 tctgtgcctg ttcaccagac gcctggagga aggccagttt atctggcctg ctgtgcgtga
      361 cggcaaggta tccattaccc gctcgcaact ggcaatgctc ctcgataagc tggactggcg
      421 tcagccaaaa acatcccgcc ttaatgcact gacaatgttg taaaaatgtc atggccggat
      481 tataaaaacg gccatgaatc aaaaatacct cattcgcatt gcagaactgg aatgccagct
      541 ccgtcagaaa gaccagcaac tgagtctggt tgaagagacg gaggccttcc tgcgctctgc
      601 actggcccgc gccgaagaaa agatcgaaga agatgaacgg gaaatagaac atctgcgggc
      661 tcagatagaa aaactgcgcc ggatgctgtt cggtacccgt tctgaaaaac tgcgtcgtga
      721 agttgaacag gctgaggccc tgctgaaaca acgcgaacag gacagtgatc gttacagtgg
      781 gcgggaagac gatccgcagg ttccccgcca gttgcgacag tctcgtcatc gtcgcccgtt
      841 accggagcat ctgccccgcg aaataaatcg cctggagcca gaagaaagct gttgcccgga
      901 gtgtggcggt gagctggatt atctggggga agtcagcgca gaacaactgg aactggtgag
      961 cagcgctctg aaagtgatcc gcacagaacg ggtaaaaaaa gcctgtacaa aatgtgactg
     1021 catcgttgaa gcaccggcac catcccgtcc gatagagcgt ggtatcgcgg gcccggggtt
     1081 acttgcccgc gtgttaacgg gaaaatactg cgaacacctg ccactgtatc gtcagagtga
     1141 aatttttgcc cgtcagggtg tcgaactgag ccgtgcatta ctctccaact gggttgacgc
     1201 gtgctgccag ttaatgacgc cgctgaatga tgctctgtac cgttatgtaa tgaacagccg
     1261 caaagttcac actgatgaca caccagtaaa agtgctggca ccgggcagga agaaggcgaa
     1321 aacaggatat atctggacgt atgtccggga tgacaggaat gccggttcgc cagagcctcc
     1381 ggcggtctgg ttcgcctact caccggacca tcagggtaaa catccggagc agcaccttag
     1441 tcccttccgg ggtatcctgc aggcagatgc gtttaatggt tacgatcggc tgttcagtgc
     1501 cgaacgagaa ggcggcgcgt tgacggaagc aggatgctgg gctcatgcgc ggcgcaaagt
     1561 ccacgatgta tatatcagta ccaaaagcgc gacagcggaa gaagccctga aactaatcgg
     1621 tgagctgtac gccatcgagc acgaaatacg cgggttgccg gtgtctgaac gcctggcggt
     1681 caggcaaatg cagagtaaac cgctactgac ttccctgtat aagctgatgc aggagaaaga
     1741 acacacgtta tcgaaaaaat gccgtctgag agatgcgttc cggtatatca ggaagcactg
     1801 ggttgcgttg tgcaacttca gtgatgatgg tctggctgag gcggataata atgccgcgga
     1861 aagagcgctt cgtgcagtct gtctcggaaa gaaaaacttt atgttcttcg gcagcgatca
     1921 cggtggagag cgtggtgcgc tactgtacgg gctgatcggc acctgccgac tgaacggtat
     1981 cgatccggaa gcgtatctgc gctatatcct gagcgtactg ccggaatggc cttccaaccg
     2041 tgttgacgaa ctcctgccat ggaacgtagc actcaccaat aaataagcgt caatacggtg
     2101 ctccgttgac gcttacgaac atcaggtctt ttatgaagat gaa
//
LOCUS       DAJTSL010000289         2107 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTSL010000289 DAJTSL010000000
VERSION     DAJTSL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.292"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            119..621
                     /locus_tag="OO433_004129"
                     /pseudo
     CDS             119..621
                     /locus_tag="OO433_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(667..1083)
                     /gene="rraB"
                     /locus_tag="OO433_004130"
     CDS             complement(667..1083)
                     /gene="rraB"
                     /locus_tag="OO433_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6052386.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1245..>2107
                     /gene="argF"
                     /locus_tag="OO433_004131"
     CDS             1245..>2107
                     /gene="argF"
                     /locus_tag="OO433_004131"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6052387.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIAETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGK"
BASE COUNT          535 a          500 c          526 g          546 t
ORIGIN      
        1 atactcttat cagtaatagg ctggcaaaaa ctttattgct ttataagatg cttgcgcacg
       61 taagaagaga gtggtattaa acaaatgtat aattacttta tttttattgg gttaaactat
      121 gaataacatt gcgccgcaat cacctgtaat gcgtcgcctg acgctacagg ataatcctgc
      181 tattgcccgc gtcattcgtc aggtatccgc cgaatacggt cttaccgctg ataaaggcta
      241 caccgtcgcc gatccgaatc ttgacgaact gtatcaagtg tatagtcagc cgggtcatgc
      301 atattgggtg gttgagtacg agggtgaagt ggtcggcggc ggtgggattg cgccattagc
      361 aggtagtgaa tcggacattt gcgaactgca aaagatgtat tttcttccgg ctattcgcgg
      421 taaagggctg gcgaaaaaac tggccttaat ggcgatggag caggcacgag agatgggctt
      481 caaacgctgc tatctggaaa cgaccgcttt ttaaaggaag ccattgcgct ttatgagcat
      541 ttgggctttg agcatatcga ctatgcactt ggctgcacgg gccatgttga ttgtgaagtg
      601 cggatgttgc gcgaactcta aaaaaatgcc cgctagtgtg tgctatcggg cattttaatc
      661 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccgtc
      721 ttcgccatta ggatcttcaa aataagtgcc ccaaccgtcg tactcaacgt caaatttctc
      781 cgccaatgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
      841 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
      901 ggtcacttcg taaccgagtt taaacgcttc aactgctgct ttttccaggg tttccagatc
      961 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
     1021 taattcttca ataatcaaac gagtttcttc acgctgttct tccagttgtt ccgggtttgc
     1081 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacatacc actgacattg
     1141 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat catccatata
     1201 aattgaattt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
     1261 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
     1321 tagccgcgaa gctgaaagcc gataagaaaa gcggcaaaga agaagccaaa ctcactggta
     1381 aaaacatcgc gctcatcttc gaaaaagact cgacccgtac ccgatgctct ttcgaagttg
     1441 ccgcatatga ccagggtgct cgcgttactt atctcggccc aagcggcagt cagattggtc
     1501 ataaagagtc gattaaagac actgcccgcg tgctgggtcg catgtatgac ggtattcagt
     1561 atcgcggcta tggtcaggag attgccgaaa cactggcgga atacgctggc gtgccggtat
     1621 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
     1681 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
     1741 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
     1801 tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt tacggaatgc cgcgccttgg
     1861 cacagcaaaa tggcgggaat attacgctga cggaagatgt agccaaagga gttgaaggtg
     1921 ctgactttat ctataccgat gtatgggtgt cgatggggga agcaaaagag aaatgggcag
     1981 aacggattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
     2041 acccggaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccag acgacgcttg
     2101 gtaagaa
//
LOCUS       DAJTSL010000290         2034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTSL010000290 DAJTSL010000000
VERSION     DAJTSL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.293"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(48..384)
                     /locus_tag="OO433_004132"
                     /pseudo
     CDS             complement(48..384)
                     /locus_tag="OO433_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(917..1243)
                     /locus_tag="OO433_004133"
     CDS             complement(917..1243)
                     /locus_tag="OO433_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052388.1"
                     /translation="MFKYAEMLMAEEDNKVCELIFKQQDNLILKINDRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLILCALGVNSWRGKSKYLDYYLLRDEMPVRLVSIIFPTLL
                     ALFLLA"
BASE COUNT          668 a          383 c          356 g          627 t
ORIGIN      
        1 ataaatatga aaagatggtt taaatacccc gttacttttg acttacacta tacggtatcg
       61 catcgtttaa tattcgcacc ggccagattt ttatttctat tagttgtcac aatactgaat
      121 gcgtacgacc acagtattct ggctcctgtg tggtcatgct ttaattctgc gttccggaca
      181 gataagcagt tgcttgcagg aatccttctt gtgttaatgt cagttcccct ttttaccagt
      241 gctgatttcc acattccgtc caacagagct tatagccttt ccctggatta tagcattgtc
      301 cggctgaagt tctttttgaa taataataga agcactgctg gcagatccag tccgtttttc
      361 ataacccact gtactgataa ccataatcta atcagtagaa attgagtcga aaataagcac
      421 tactccatac aggataatta gaggtcagtt tgattattca caattcatta tcagcatttt
      481 ctgtttctga cgaaatcaac atgaaaagaa ccatatatga taattattat aataacggct
      541 ttaattggaa tacatatatt acaacgtatt atatataatt ggtattctgg gaactatatt
      601 ctcaaaatac agtagaaacg aggtatgttt ctggtggaaa ggacagtggg attaaaaagt
      661 aagaattgat aaaaaaacgc cagcaacaca tagtgccggc ggagggaatc ccccatggag
      721 aaaatgtgat gcctagagca ccatgaatat ataattaaaa atagttagag ttgtcaatac
      781 tttcacaaaa ataattttcg tagtttcgta gtatagaaag atatttttat gcatgaccta
      841 cctgaatttg ctccgggtag aggttataaa taaaaattga atcacgacaa acacaatatt
      901 catagtatgg cgatgcctac gccagcaaga atagcgccaa taatgttggg aatataatag
      961 acaccagacg cacaggcatc tcatctctta acaaataata atcaaggtat ttacttttgc
     1021 ctcgccagct gtttacgcct aaagcacaca agattaacca ttcaataaca aatggcaata
     1081 tccatagcca tacgacttta ccttgtaatg ttcggtattt ctttataatt attctgggaa
     1141 atctatcatt tatttttaaa atcaaattat cttgttgttt aaaaataagt tcacatactt
     1201 tattatcttc ttccgccatc aacatctctg catacttaaa catttcagaa cgttccttta
     1261 gcacagaaga gtaattatat gtccagttcc caggcaataa tgcttatgga tatttaacta
     1321 ataatttaga gaatattttg caataatatt tggctgtatt cagaaatacc tgaaaaatca
     1381 tactatacag ccccagggaa tggataaaga ttctaacaaa gcatttcaac aatatatgct
     1441 tgttaaaaat ccatatcgaa atgccgttgc agcattaata tatgcctcat tcataaaatg
     1501 taaaagagca agctcgtacc agtagggggg aaattcattg acatgtcctg tcaatacgta
     1561 cagagccctg tcatgcttgc cagcacgtaa caattccgtc cccagtaacc agcgtgtccc
     1621 ctgattattc tcagggttat atgttagtat tttatcaatg agtataacgg catcctgatg
     1681 acgctgtaaa tgaacattcg ccagaattgc agcataaaga gcgcaaatgc atatgccaga
     1741 tgagcgtgaa tatcaatgat gtcaggagca tgtcaggcaa gacgcctgag tttgttgacg
     1801 taatttgttt cgtttatttc atcactatcg tatgcgtcaa gagttttttc aaactcatcc
     1861 cgaaatgggc caagcctggt ttcaggaaat aagaaatacc cctagttatt gttcacttaa
     1921 aagtataatt tgtaattcaa atcctgagcc tgtaaaacgg ggagtaacat attctattct
     1981 gaagagaata aaagtcgtga tgcgatgtat caagcccgga ttgtaatccc agat
//
LOCUS       DAJTSL010000291         2010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTSL010000291 DAJTSL010000000
VERSION     DAJTSL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.294"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..84)
                     /locus_tag="OO433_004134"
     CDS             complement(<1..84)
                     /locus_tag="OO433_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HCR6052389.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVN"
     gene            326..619
                     /locus_tag="OO433_004135"
     CDS             326..619
                     /locus_tag="OO433_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6052390.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            691..1350
                     /locus_tag="OO433_004136"
     CDS             691..1350
                     /locus_tag="OO433_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6052391.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1442..1888
                     /locus_tag="OO433_004137"
     CDS             1442..1888
                     /locus_tag="OO433_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6052392.1"
                     /translation="MNDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PVWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          530 a          432 c          523 g          525 t
ORIGIN      
        1 attgacattc ttacccgcag caaagctcgt ggaggttagc agtgcaaaca gcaatacagc
       61 cttactgatg ctcttgattt tcattggtag tctcctgttc agaatgagta cgatgaactg
      121 ttataatata acaatcccta acgggagaat tccagtggct tttctgcgtt cggtaaaatc
      181 ccggcgcggc attttttgcc ggtgggatta cttataaagg ggcatggact caacaaaccg
      241 cagaaatcta cagctggctg gcttgtggcg cgggtttcat tgatgttagc ttatgccaaa
      301 acctggcatc ttaaaaagta aaagtatgtt ttgtgtgatt tatcgaagca gcaagcgtga
      361 ccagacctat ttatatgtcg aaaaaaaaga cgatttttcg cgtgttcctg aggaactgat
      421 gaaaggtttt ggtcagcctc agttagcgat gattctgccg ttggatgggc ggaagaaact
      481 ggtcaatgct gatattgaaa aggttaaact ggcattaacc gagcaaggtt actatttgca
      541 gttaccgcca ccacccgaag atttgctgaa gcaacatctt tccgtcatgg ggcagaaaac
      601 agacgacacg aacaaataac cgatatccgg cggtggcatt atctttgtcg gcgcgggttt
      661 tcatatccac gataaggtga ggggaacgtt atgtatcaac atcacaactg gcaaggtgcg
      721 ctgctggatt atccggtgag taaagtagtc tgtgttggca gtaactatgc caaacatatt
      781 aaagagatgg gcagcgcagt gcccgaagag ccagtgctgt ttattaaacc agaaacggca
      841 ctgtgcgatc tgaggcagcc attggcgatc ccatccgatt tcggttcagt tcatcatgaa
      901 gtcgaactgg cggtgttgat tggtgcgacg ctgcgtcagg caacggaaga gcatgtccgc
      961 aaagccattg ccggttacgg cgtggcgctt gatctgacat tgcgtgatgt tcagggaaaa
     1021 atgaagaaag ccgggcagcc gtgggaaaag gctaaagcgt ttgataactc ttgtccgctt
     1081 tccgggttta tccccgcggc ggaatttacc ggcgatccac aaaatacaac gctgggcctg
     1141 agcgtaaacg gcgaacaacg ccagcaaggt acgactgcgg acatgatcca taaaatcgtt
     1201 ccgttgatcg cttatatgag caagtttttt accctcaagg ccggtgacgt tgtgttgaca
     1261 ggcacgcctg atggcgtcgg cccgttgcaa agcggtgatg agctgacagt cactttcgat
     1321 gggcattctt tgacaactcg cgttttgtaa tactttttgc cgcctgaaag cggcggcaaa
     1381 acttgcatcg ctgtgccaga ctggttataa ggtgcgtttt aacgtaatgg cggaacacct
     1441 gatgaacgat gtacctttct ggcaaagtaa aaccctggac gaaatgagcg atgcggaatg
     1501 ggagtcgttg tgtgatggtt gcggtcagtg ttgcctgcat aaactgatgg atgaagacac
     1561 cgacgaaatc tacttcacta acgtcgcctg tcgccagctc aatattaaaa cctgtcaatg
     1621 tcggaactac gaacgtcgtt tcgagtttga acccgactgc attaaattaa cccgtgaaaa
     1681 tctgccaaca ttcgaatggc tgccaatgac ctgcgcttat cgtttgctgg cggaaggtaa
     1741 agatttacct gtgtggcatc cgctacttac tggttcgaaa gcggcaatgc atggtgaacg
     1801 tatctctgtg cgtcatatcg cagtgaaaga atcagaagtg attgactggc aggatcatat
     1861 cttaaataaa cctgactggg cgcagtgata aatttaatag atgttgatgg gggctttctt
     1921 aatatacgtc tggatgctcg ccatttcgta gccctgtttt tgaaagcgct ctgtcataat
     1981 gagagttacc cgatacctta ttatcaacag
//
LOCUS       DAJTSL010000292         2005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTSL010000292 DAJTSL010000000
VERSION     DAJTSL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.295"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            61..582
                     /locus_tag="OO433_004138"
     CDS             61..582
                     /locus_tag="OO433_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052393.1"
                     /translation="MDVYANGQHVFRFQNGVAIALKNIQAGNAKKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELGDDSGWHFYSQRNTDGSITFAVNGQVIPLNYGNFDARYKHRT
                     EGVQDVRYGYEMYYNPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQ
                     KLINGIWYNVASI"
     gene            585..1130
                     /locus_tag="OO433_004139"
     CDS             585..1130
                     /locus_tag="OO433_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6052394.1"
                     /translation="MLHLKNITAGNPKTVEQYQLTKQYDVTWLFSKDGKNWYEELKNF
                     ASDTIKITYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFS
                     VITDKTSYNAIEWPVSPEGSS"
     gene            1154..>2005
                     /locus_tag="OO433_004140"
     CDS             1154..>2005
                     /locus_tag="OO433_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6052395.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLTAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAQNDIDAALTGTLKMAN
                     HLSEIAAAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYDIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYG"
BASE COUNT          547 a          460 c          544 g          454 t
ORIGIN      
        1 gggaattcaa ttgctttcgg ggataacgat acaggtataa aacaaaacgg cgacggcatt
       61 ctggatgttt atgcgaatgg acagcatgta tttcgtttcc agaatggtgt ggcgatagcg
      121 ttaaaaaata ttcaggccgg aaatgctaaa aaattcacgt tatccagcgc caacaactcc
      181 acgaaaaatg cagcgtttta tttgtggggt aatccatcca ggcctgttgt tgcagagctt
      241 ggtgatgatt ccggctggca tttctacagc cagagaaata ccgatggtag tatcacattc
      301 gctgtaaacg gacaggtaat tccgttaaat tacggaaact tcgatgcccg ctataaacat
      361 cgaactgagg gggtacagga tgtgcggtat ggttatgaaa tgtattacaa ccctggcagc
      421 aacaccgttt catggacgtt tcgctcacct tcgggacacg ggttgtcagg gatatcgata
      481 tcggataccg gccgtaactc agcggataac gtcaacggtg tgtattaccg accgctgcaa
      541 aaactgatta atggcatctg gtataacgta gcgagtattt aacaatgttg catttaaaaa
      601 atattacggc aggtaatccg aaaaccgtgg aacaatatca gctgacaaaa caatatgatg
      661 tcacctggct tttttcgaaa gacggaaaaa actggtatga agagctgaag aatttcgcca
      721 gcgacacaat taaaataact tacaccggag acggtcgcgt ggtgtgggtc ggtaaggatg
      781 tgactggcat tgaaccccgt aacgccagtg ttattgaagt tcctgatatt accgccaacc
      841 gccgcattac cgcgccgggt tactggtttt accgcaatga tgaatttgtc tttgactaca
      901 gactcaaagc ggaagatgag cgtgatgccc ttctggctca ggtcagtgcc cggacagggg
      961 aatgggaaga agacctgctg ctggggctaa tcagcgacga agaccgggaa aaactgaaag
     1021 cgtaccgcat ttacgcgaaa tcgctgcagg cgatggattt cagtgtcatc actgataaaa
     1081 cctcatacaa cgccattgaa tggcccgtct ctccggaagg ttcttcctga tttaatttat
     1141 cgcgagaaaa actatgtctg tagtgatatc aggtgcgctg actgatggcg caggcatccc
     1201 catgtccgga tgccagatta ttctgaaatc ccgtgtaaac acctcagaag tggtgatgcg
     1261 taccagggct gatgtagtga ccggaaataa cggtgaatat tcgtttgagg cacaggtcgg
     1321 aaaatattgc gtgtatctga aacgggactg gcgcgacgag tactgtgttg gcgacattgc
     1381 tgtatacgac gactcaaagc ccggcacgct gaatgacttt ctgactgccc ccgatgaagg
     1441 cgacctgaag ccggatgtcg tcaaacgctt tgaggaaatg gtggcgcagg cgcagcagag
     1501 cgccggggcc gcagccggaa acgcacagca gacggcgcag gatgtggcgg cagccgcagg
     1561 ttatgcccgc gcagcagaac aggcccaaaa tgacattgat gctgcgctga ccggcactct
     1621 gaaaatggct aaccatctgt cagaaatcgc agcagcaggc gaaaaggcac aacagaagtc
     1681 ccgggataat ctggggctga aaagtgcggc cacgatggaa gcacagagcg atatttacga
     1741 ccggacaaaa ggccgtctgg cgatacccgg cgcattcggc tttgggtgtg cttttctgcc
     1801 tgaagatgtt atccgttttg acactaagag tgatttcctg gcctgggtaa ggaatgcgct
     1861 gccaggtgaa tattccgttg ctggccccta cgacatcatc atacccgaca cacggtttga
     1921 aggggtgctc agcatccggt ggactgatgc acgccctgag acaacagaac cgcggtacag
     1981 agccaaatcc cttacttttt acggc
//
LOCUS       DAJTSL010000293         1977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTSL010000293 DAJTSL010000000
VERSION     DAJTSL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.296"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1954
                     /gene="yicI"
                     /locus_tag="OO433_004141"
     CDS             <1..1954
                     /gene="yicI"
                     /locus_tag="OO433_004141"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6052396.1"
                     /translation="KGEFWSLDFLRNGERITGQDTNNQRNYMFERLDLGVGETVYGLG
                     ERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQSVSFEVGSEK
                     VSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTSFTTNYD
                     EATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRRLKAKGL
                     KICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNPDACKWY
                     ADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAHIYNELVWNVLKDTV
                     GEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFWSHYIGS
                     FENTAPAHVYKCWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLKCRMMPY
                     LYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAGDVQFYL
                     PEGRWTHLWHNDELDGRCWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAWHEGTAF
                     HLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRNVVKVNG
                     LQDGSQAESEQGLVVKPQGNALTITL"
BASE COUNT          452 a          477 c          563 g          485 t
ORIGIN      
        1 caaaggtgag ttctggtcac tcgattttct gcgcaacggt gaacgtatta ccggtcagga
       61 cacgaataat caacgaaatt atatgtttga gcggcttgat cttggcgttg gcgaaacagt
      121 ttatggtctg ggagagcgtt ttactgccct ggtgcgcaat ggtcagacgg tagagacctg
      181 gaaccgggac ggcggcacaa gtactgaaca ggcgtataaa aatatcccgt tctatatgac
      241 taaccgtggc tatggggtgc tggtcaatca cccccagagt gtctcttttg aagtgggatc
      301 ggagaaagtc tccaaagtgc agttcagcgt tgagagcgaa tatctcgaat actttgttat
      361 cgacggtccg acgccgaaag cggtacttga tcgttatacc cgttttactg gtcgtccggc
      421 gctgccgccc gcatggtcct tcggcctgtg gctaaccact tcatttacca ccaactacga
      481 cgaagcgacg gtaaacagct ttatcgatgg tatggcggaa cgcaatctgc cgctgcatgt
      541 tttccacttt gactgtttct ggatgaaagc cttccagtgg tgcgattttg agtgggaccc
      601 gctgactttc ccggacccgg aagggatgat ccgccgcctg aaagcgaaag ggctgaaaat
      661 ctgcgtctgg attaacccct atatcggtca aaaatccccc gtctttaaag agttacaaga
      721 gaaaggctat ttactcaaac gcccggacgg ttcgctatgg cagtgggata aatggcagcc
      781 aggtctggcg atttatgact ttaccaatcc ggatgcctgc aaatggtacg ccgacaaact
      841 gaaaggtctg gtcgcgatgg gcgttgattg ctttaagacc gactttggcg aacgtatccc
      901 aactgatgtt cagtggtttg acggttccga tccgcagaaa atgcataacc attatgcgca
      961 catctacaac gaactggtgt ggaacgtgct caaggacacc gttggtgagg aagaagctgt
     1021 cttgtttgcc cgctcggcct ccgtcggtgc gcagaaattc ccggtacact ggggtggcga
     1081 ttgttacgct aactacgaat caatggcgga aagcctgcgc ggtggtttgt ctattggcct
     1141 ttcaggtttt ggcttctgga gccactatat cggcagcttt gaaaataccg ctccggcgca
     1201 cgtttacaaa tgctggtgcg cgtttggttt gctctccagc catagccgtt tacacggtag
     1261 caaatcttat cgtgtgccgt gggcctacga tgatgagtcc tgtgatgtgg tgcgcttctt
     1321 cacgcaactg aaatgccgca tgatgccgta tctgtatcgt gaagctgcgc gtgcaaacgc
     1381 gcggggtacg ccgatgatgc gggccatgat gatggagttc ccggacgatc cggcttgtga
     1441 ttaccttgac cgtcaataca tgttaggcga caacgtgatg gttgcgccgg tgttcactga
     1501 agcgggcgat gtgcagttct acctgccgga aggtcgctgg acacacctgt ggcacaacga
     1561 tgagctcgac ggtcgttgct ggcataaaca gcagcacagc ttcctgagtc tgcccattta
     1621 tgtgcgtgat aacaccctac tggcgctggg caacaacgat caacgtcccg attacgcgtg
     1681 gcacgaaggc acggcattcc acctctttaa tctgcaagac gggcatgaag ccgtctgtga
     1741 agtgcccgct gctgacggat cggtgatctt tactttaaaa gcagcacgta ctggcaacac
     1801 gattactgtg actggtgcgg gcgaggcgaa gaactggaca ctgtgcctgc gcaatgttgt
     1861 gaaagtaaat ggtctgcaag acggttcgca ggctgaaagt gagcaggggc tggtggtgaa
     1921 gcctcaaggg aatgcgctga caattacgtt gtaatctatt gatggaatgc cgggtac
//
LOCUS       DAJTSL010000294         1976 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTSL010000294 DAJTSL010000000
VERSION     DAJTSL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.297"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            547..1536
                     /locus_tag="OO433_004142"
     CDS             547..1536
                     /locus_tag="OO433_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR6052397.1"
                     /translation="MKNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGA
                     RMASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPR
                     IVADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVT
                     RGSDGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRK
                     PKDGESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGAL
                     KDVLKLDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
BASE COUNT          439 a          527 c          585 g          425 t
ORIGIN      
        1 aaaaaaaatt cggcgtcccg ctcactgttc ccgaactcgt caaacagaaa atcgcggggg
       61 cccctccggg gttccgattt tgtgattgac gacggcacgg aaaaaaacac tggggatggt
      121 ggttggtgtc acgcagtgag gccaaccacc tgcaagacgg tttttgtttt tcacaaaatc
      181 gctggaaaaa cctgtgagtg ttttgcgggg aaaacgcttt tgtggttgtt gtttttgctg
      241 tccgaaaatc agctcagatt ttgagcaaat ctcgcgcgcg ttccttgtat ttatacttaa
      301 gggataactc taggattcaa attggcctcc agcccttgtc ccgcaaggcc tgagggggga
      361 tttgtcggaa aatggttttg gcgttattct gtcgggagtg gttgcgttaa aagtgacccc
      421 cgcaacgacg ggcatcgttc gggggtgtga ccacctgtag aaattcgaat tagtgacacg
      481 acaacacagg tgatatggca atgataacac gtcagatgag ccatcgccca caatcgggtg
      541 gtgcgcatga aaaaccctaa actccagtac ctcactgatt attccccctc tgatgcgccc
      601 tgggacgcac acagaagcgt ttctgacgac gtgggaggga tttacctgct tgcggccgaa
      661 tatgagcgct acggggctcg tatggcctcc tgtggcggtt tactgcgttt cgggtggtct
      721 accctcaagg aaaccggaga aacgcgtctg aggctgcgtg aggcacattt ctgccgtgtc
      781 cggcactgtc ctgtctgcca gtggaggcgc tccctgatgt ggcaagcccg tttctaccag
      841 tccctgccgc gtatcgtggc ggattatccc gatgcccgct ggatgttcct gaccctgacc
      901 gtgcgcaact gtgcgattgg ggaactgggc gagatgctga accggatgaa tgcagctttt
      961 cagcgtatga aggttcgcaa ggagttcctg cctgttcagg gctggatccg cacaacggaa
     1021 gttacccgcg gcagcgacgg cagcgcacac ccgcacttcc ataccttgat gatggctcct
     1081 cctggcatgc taaatgggaa aagttacgtc aggcatgaac gctgggttga gctatggcgg
     1141 gagtgcctgc gggttgatta tgacccgaat gttgatgtcc gggcagtgaa accccgcaag
     1201 ccgaaggatg gcgagtcctt agcctgcgcg accgctgaac tggtgcgcgg cgcagtggcc
     1261 gaaaccctga aatattccac gaagccggct gacatggtgg ccgatcctga atggttcctc
     1321 gaactgaccc gccagacgca caagcgccgc tttgtggcga ccggcggcgc actcaaagac
     1381 gtgttgaaac tcgatcagga aaccgacgcc gacatggtga tcggggatga tatttccgag
     1441 ggcgacgatg acggttcacg aatcgcgttc gagtggaaaa ccgaggtcag aaagtaccgt
     1501 cgctcaccga cgaaagataa aactgaatca ggctagcggg gtgtcggggc gaagccctga
     1561 ccaggtgggg aatgtctgag cgagcgtgcg cggtccgaca ttcccacatc ctgtcccgaa
     1621 aattcccgcc gtttctcggt tttttgagcg tcgcagaccg cacgacaaac gaaacgaagt
     1681 gtagtttgta tgagtgcgca cttcgtgaga ttccccgact gccagtttgt cgatcgcgcg
     1741 gaacagctgc ggcacgaaaa cggcagaatg atttgctgtc cggttgccag aactgtgcgc
     1801 ttacgtggag ggcggcgcgg cgcaaaaact gccgacattg cgccagtgtt ccggtgacac
     1861 aaacccgcag aaatcgggac aggatgtgta atcgtataac cgcgcacgca cagccatccg
     1921 attaccacct ggtcagggct tcgccccgac accccgccaa cgcttcacgg cacgcg
//
LOCUS       DAJTSL010000295         1944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTSL010000295 DAJTSL010000000
VERSION     DAJTSL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.298"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            27..260
                     /locus_tag="OO433_004143"
     CDS             27..260
                     /locus_tag="OO433_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6052398.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(261..710)
                     /locus_tag="OO433_004144"
     CDS             complement(261..710)
                     /locus_tag="OO433_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6052399.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(707..1225)
                     /gene="mddA"
                     /locus_tag="OO433_004145"
     CDS             complement(707..1225)
                     /gene="mddA"
                     /locus_tag="OO433_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6052400.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1406..>1944
                     /gene="curA"
                     /locus_tag="OO433_004146"
     CDS             1406..>1944
                     /gene="curA"
                     /locus_tag="OO433_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6052401.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSHVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGG"
BASE COUNT          507 a          494 c          526 g          417 t
ORIGIN      
        1 atttcacaag tggttaagga gaaagtatgt cgcatctgga tgaagtcatc gcgcgcgtgg
       61 atgccgcgat tgaagagagc gtcattgccc atatgaacga attattaatc gccctgagcg
      121 atgacgcgga gttaagtcgg gaagatcgct acacccagca gcagcgtttg cgcaccgcta
      181 ttgctcatca cggtcgcaag cataaagaag atatggaagc gcgccacgaa cagttaacca
      241 aaggcggcac catcctctga ttaaaatgag cgtctggcta ccaaccaggc gccaaccaca
      301 agtaaaattg cgccacaaat tgggccgaac aacgcccgta gtggtacgcc atgactgcgg
      361 aagatatcca gcatcagacc gccaattagc tggctggcga ccagtactgc aatagttgtc
      421 gccgctccca cattctgata tccgctgata ctggcgaaga caaaaaacga tcccagcaag
      481 ccgggtataa gtgtccacca gcgcacgctg gacaccagtt cgccaaaccc cgccatgcct
      541 tgtttaaacc ataaaataga aacaaagagc acaatcccca ccagtgagtt cagcaacatg
      601 gcgatgagaa tggtagagga ggtctgggtg atgcgcacca tcagcgtgtt ctggactacc
      661 agaccaattc cggcggcaat caaaaaagca agggtgagcg actgattcat ccaatcgcgt
      721 ccggttcagt gcgttcgtcg agttgcaact gcataaatgt cagatccagc cagcgcccaa
      781 atttagtgcc cacctgcggc atttgtgcgg tgacgacaaa tcccagcgaa tggtgcagat
      841 gcagcgaggc ctgattttgc gattcgatcc cggcgaccat gacatgcttc ccgctatccc
      901 gcgcttcatc aatcaatcgg cttaacaatt tacgccccag acctttgccc tgatgatcgg
      961 gatggacata aaccgaatgt tccacggtat ggcgaaaacc atcgaaacta cgccagtcgc
     1021 caaacgaggc atatcccgtc actacgccat cttcttcact taccagcacc ggataacctg
     1081 ctatggtccg cgcttcaaac caggcaatgc ggttatctgc atcaaccgtt tggtcattcc
     1141 agatagccgc cgtatacaac acggcgtggt tataaatttc cgcaatggca gcacaatcgg
     1201 ctttgcgggc aaaacggatg gacatgtctg gcctcgataa aataatgatg atgatgttat
     1261 caatactatc gaaaacagcc cgaagcaacg gattcctcga tgaaattatt gcgtgttgca
     1321 gaggggatgt gacggctgca aacaaaacca atcccgtgag cctgctacta ttgatggatt
     1381 catgccaaaa gcacaggaga gcattatggg gcaacaaaag cagcgtaatc gacgttgggt
     1441 tctggcctcg cgtccacatg gcgcaccggt tccggagaat ttccgtcttg aagaagatga
     1501 tgtcgccaca ccgggtgaag gacaggtgtt actgcgcaca gtttatttgt ccctagaccc
     1561 gtatatgcgt ggacgtatga gcgatgagcc atcttattca ccgcctgttg atattggcgg
     1621 cgtgatggtc ggcggcacgg tgagccatgt tgtggagtcg aatcatcctg attatcagcc
     1681 tggcgactgg gtgctgggct acagtggatg gcaggactat gacatatcca gtggtgatga
     1741 tctggtgaaa cttggcgatc atccgcaaaa tccatcgtgg tcgctgggtg tgctggggat
     1801 gccaggcttt accgcttata tggggctgct ggatatcggt cagcctaaag agggcgaaac
     1861 gttggtggta gctgcggcga caggtcctgt gggagcaacg gtggggcaaa tcggcaaact
     1921 taaaggttgc agggtggtgg gggg
//
LOCUS       DAJTSL010000296         1934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTSL010000296 DAJTSL010000000
VERSION     DAJTSL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.299"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="OO433_004147"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="OO433_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6052402.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1149..1520)
                     /locus_tag="OO433_004148"
                     /pseudo
     CDS             complement(1149..1520)
                     /locus_tag="OO433_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1519..1934
                     /locus_tag="OO433_004149"
                     /pseudo
     CDS             1519..1934
                     /locus_tag="OO433_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          608 a          347 c          386 g          593 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc cttttggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ctagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac
     1261 gactggagtt gcttctgcgg tgctctggtg gcgaatgtga agtcatactc ttcgaacgtt
     1321 ttcaccgccg ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc
     1381 agtttttctt catgaagcag atgctccagg aagtccatat aactccattc ctggtctact
     1441 gcctgttgtg acagcgcagg cgctgcgctt atgtactgca cccattttgt tggacgatga
     1501 aatggaatag cccctaatat gtcaaagcca aaataccctt ttgaaaagcg ccttgaagtc
     1561 gtgaatcact acttcactac tgatgatggt tacaggatca tctcggcacg ttttggtgtc
     1621 cccgaaccca ggtcaggaca tgggttgccc tctatgaaaa acatggagaa aaaggcttaa
     1681 ttcccaaacc taaaggtgtt agtgctgatc cagagttgcg tattaaggtc gtgaaagctg
     1741 tgattgagca gcacatgtcc cttaatcagg ctgctgctca ctttatgctt gctggtagtg
     1801 gttctgtagc caggtggctg aaggtttatg aagagcgcgg agaagctggt ttacgcgcgc
     1861 tcaagattgg caccaaaaga aacattgcaa tatcagttga tccagaaaaa gcggcatcag
     1921 cattggagct gtca
//
LOCUS       DAJTSL010000297         1873 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTSL010000297 DAJTSL010000000
VERSION     DAJTSL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.300"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>1873
                     /gene="ppsA"
                     /locus_tag="OO433_004150"
     CDS             <1..>1873
                     /gene="ppsA"
                     /locus_tag="OO433_004150"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6052403.1"
                     /translation="DASFAGQQETFLNVQGFDAVLVAVKHVFASLFNGRAISYRVHQG
                     YDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNP
                     DEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTN
                     EEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHS
                     QGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKASAIV
                     TNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFS
                     VKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALL
                     EFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNE
                     YANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIM
                     IPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGS
                     NDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHE"
BASE COUNT          412 a          475 c          566 g          420 t
ORIGIN      
        1 cggacgcttc ctttgccggt cagcaggaaa ccttcctcaa cgttcagggg tttgacgccg
       61 ttctcgtggc agtgaaacat gtatttgctt ctctgtttaa cggtcgcgcc atctcttatc
      121 gtgtgcacca gggttacgat caccgtggcg tggcgctctc cgccggtgtt cagcggatgg
      181 tgcgctccga cctcgcatct tctggcgtga tgttctccat tgataccgaa tccggctttg
      241 accaggtggt gtttatcact tccgcatggg gccttggtga gatggtcgtg cagggggcgg
      301 ttaacccgga tgagttttac gtgcataaac cgacactggc ggcgaatcgc ccggctatcg
      361 tgcgccgcac catggggtcg aaaaaaatcc gcatggttta cgcgccgacc caggagcacg
      421 gcaagcaggt taaaatcgaa gacgtaccgc aggaacagcg tgacatcttc tcgctgacca
      481 acgaagaagt gcaggaactg gcaaaacagg ccgtacaaat tgagaaacac tacggtcgcc
      541 cgatggatat tgagtgggcg aaagatggcc acaccggcaa actgttcatt gtgcaggcgc
      601 gtccggaaac cgtgcgctca cgcggtcagg tcatggagcg ttatacgctg cattcacagg
      661 gtaagattat cgccgaaggc cgtgctatcg gtcatcgcat cggtgcgggt ccggtgaaag
      721 tcatccatga catcagcgaa atgaaccgca tcgaacctgg tgacgtgctg gtcactgaca
      781 tgaccgaccc ggactgggaa ccgatcatga agaaagcatc tgccatagtc accaaccgtg
      841 gcggtcgtac ctgtcacgcg gcgatcatcg ctcgtgaact gggtattccg gcggtcgtgg
      901 gctgtggtga tgcaacagaa cggatgaaag acggtgagaa cgtcactgtt tcttgtgccg
      961 aaggtgatac cggttacgtc tatgcggagt tgctggaatt tagcgtgaaa agctccagcg
     1021 tagaaaccat gccggatctg ccgttgaaag tgatgatgaa cgtcggtaac ccggatcgag
     1081 ctttcgactt cgcctgtctg ccgaacgaag gcgtgggact tgcgcgtctg gaatttatca
     1141 tcaaccgtat gattggcgtc cacccacgcg cactgcttga gtttgacgat caggaaccgc
     1201 agttgcaaaa cgaaatccgc gagatgatga aaggttttga ttctccgcgt gaattttacg
     1261 ttggtcgtct gactgaaggg atcgcgacgc tgggtgccgc gttttatccg aagcgcgtca
     1321 ttgtccgtct ctctgatttt aaatcgaacg aatatgccaa cctggtcggt ggtgagcgtt
     1381 acgagccaga tgaagagaac ccgatgctcg gcttccgtgg cgcgggccgc tatgtttccg
     1441 acagcttccg cgactgcttc gcgctggagt gtgaagcagt gaaacgtgtg cgcaacgaca
     1501 tggggctgac caacgttgag atcatgatcc cgttcgtgcg taccgtagac caggcgaaag
     1561 cggtggttga agaactggcg cgtcaggggc tgaagcgtgg tgagaacggg ctgaaaatca
     1621 tcatgatgtg tgaaatcccg tccaacgcct tgctggccga gcagttcctc gaatatttcg
     1681 acggcttctc aattggctca aacgacatga cgcagttggc gctcggtctg gatcgtgact
     1741 ccggcgtggt gtctgaactg ttcgatgagc gcaacgatgc ggtgaaagca ttgctgtcga
     1801 tggctatccg tgccgcgaag aaacagggca aatatgtcgg gatttgcggt cagggtccgt
     1861 ccgaccacga aga
//
LOCUS       DAJTSL010000298         1834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTSL010000298 DAJTSL010000000
VERSION     DAJTSL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.301"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..569
                     /locus_tag="OO433_004151"
     CDS             <1..569
                     /locus_tag="OO433_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6052404.1"
                     /translation="LAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITL
                     RKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARHLTEVACRAFASAAHLAAYAG
                     LAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQ
                     ALIALARRRCDVLFAMMRDGTFYTPQGS"
     gene            complement(940..1206)
                     /locus_tag="OO433_004152"
     CDS             complement(940..1206)
                     /locus_tag="OO433_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052405.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPATNRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
     gene            complement(1388..1786)
                     /locus_tag="OO433_004153"
                     /pseudo
     CDS             complement(1388..1786)
                     /locus_tag="OO433_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          441 a          445 c          418 g          530 t
ORIGIN      
        1 aacttgcgcc tcgtctgggt aaacgccttg cagcagacat agctcaggca ctggccgaac
       61 aaaccgtcgt cgttcccggc acgaatgccg ctgccgtagt actgccacgt ctggcactcc
      121 agctcatcac gctgcgtaag caaagagacg aggtggcgct tgaggtagaa cagcgagttc
      181 ttgctcaccc tctttacccg gtcctgacca gtatgcccgg agtcggtgtc aggaccgcag
      241 ccagacacct caccgaggtc gcctgccgcg ccttcgcctc tgccgcacat ctcgctgctt
      301 atgctggcct tgcgccggta actcggcgat ccggctcgtc aatacgcggt gagcatccct
      361 cgcgacgggg taataaagct ctcaaacggg cgttgttcct gtcggccttc gccgcgctca
      421 gggatccgct ctccagggct tactacacgc gcaaaatgag tcagggaaaa cgacacaatc
      481 aggcgcttat cgccctggcg agacgacgct gcgacgttct gttcgccatg atgcgcgacg
      541 ggacttttta taccccgcag gggtcataac atgcttgaca acttaatagg ggcacccccc
      601 aaagccgtct atgggaagga gtctgctgtt attccagaga atggtaattc agtcatatat
      661 ttctccatct atgacttgtc aataccatcc tgatatttgt gggtcagcaa tatattctac
      721 taaaaggtat ttaaggtcat tgcgtccctg cactcgaata atggtgaaga aagcatgatg
      781 aatatatcac gataacgttc agtatgtatc gaggatagat ggagttactc ctctctgaaa
      841 tagaattact gctctacttt ttctgtttcc agaaattatt agtaacaaac cctgatatat
      901 tcttttgaaa atggagctta aacgagtctg aaattaactt cagatattag aatgccgttg
      961 gctttgtttt tcaacaattc ctacttcatt tatctttgtc aaccattgca tttctgtagc
     1021 agtgaatttc aacgttttcg tccggttggt agcaggtatt tgtaattcgt atatagctgc
     1081 ttttttattg aaaacagtat atccatctgt agtaacagta gaagataata atacaggatt
     1141 tgacacttca aggatgatat tttcttgttt ttgtggaagg caagggaata tagtttgtgt
     1201 aagcatattg cttccttatg ttatatttta gtaaagaatt tattttttac attatagtcg
     1261 gctaaatgaa gtttactgta aaaaaacgat acttttttcg taagcgatga gatgtggagc
     1321 cagaaaaatg gctgcgttac gtcattgaac tgtccttgac ccacgatatt cagacagtcg
     1381 ttgtatctta agttgacatt acccgctgac gtcgatattc ccttggtgag agataaccca
     1441 acgcactatg cggatggttt tcattgtaat gctcgatcgc cactgcaagg ttatgcaatg
     1501 ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt catcgttttc acgaaccgtt
     1561 ctgctattcc attactctga gggctactga ctgccgtcgt gcagggctcc agattcaact
     1621 ctctggcgaa ctgccgtgtt tcatgagccg tataagccga accgttatcg gccactggat
     1681 tggccgttcc ggtcctttat cgccatatcg ctgacatgac atcctgcacc gttgctttgt
     1741 cataatctcc cgtgcctgct gcccagtcta tggcttcacg atcgcaatat ggaacgtaac
     1801 gctgtcatcg ggagaatcgt caatgcggca ttcg
//
LOCUS       DAJTSL010000299         1784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTSL010000299 DAJTSL010000000
VERSION     DAJTSL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.302"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..250
                     /locus_tag="OO433_004154"
     CDS             <1..250
                     /locus_tag="OO433_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCR6052406.1"
                     /translation="EFRVRAADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMM
                     TWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            264..491
                     /locus_tag="OO433_004155"
     CDS             264..491
                     /locus_tag="OO433_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6052407.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(534..>1784)
                     /gene="sbcB"
                     /locus_tag="OO433_004156"
     CDS             complement(534..>1784)
                     /gene="sbcB"
                     /locus_tag="OO433_004156"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6052408.1"
                     /translation="QPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMPPLVHVSGMFGAWSGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPTDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          429 a          448 c          459 g          448 t
ORIGIN      
        1 tgagttccgc gttcgcgcag ctgatgcaca aacaacatta cgtagcgggt taggtggtgt
       61 actgatgggc ttcggtgcca gtattgcagg tggttgctct atcggtaatg gactggttat
      121 gactgcaatg atgacctggc agggctggat tggccttgta tttatgattc tcggagtctg
      181 gactgcatcc tggcttgtgt atgttcgacc gcagcgtaag gcgcgactgg cgactgctgc
      241 ggcaaattaa gtaaggtgta aagatggcga ttaaaaagct ggatgtggtg acgcaggtct
      301 gtccgtttcc actcatagaa gcaaaagcag cactggcaga aatggccagc ggcgatgagt
      361 tggtaattga gtttgattgc acccaggcaa ctgaagcaat cccacagtgg gcggcagaag
      421 aaggacatgc catcaccgat tatcagcaga ttggcgatgc agcgtggagc atcaccgttc
      481 aaaaagcctg actcaacatt gtcctccgcc gtaccagcgg cggaggcttc aaattagacg
      541 atctcttccg cgtactgcca aagtgctttt aacagcgcca ctttttcttt gtcatcggca
      601 tattgttgta ccagcatctg caattcatca gcataaccct gcaaaaactc tggcgtgaat
      661 acctggcggc ggtgctccag ccagcgttgc tgctcggcat aatccagcgt ccccgggaag
      721 ttgcgtgccc gataattgaa caacagcttt tcaatccgtt tatcaacaaa agtgatatcc
      781 agtgccggta aattacgtgg ctcggtttcc agcacaattt tcattgctgc acgatctgcg
      841 tcactgaaaa agccgttata aagctgtgca tccacgttat ctgtaggcgt aaacggttcg
      901 gcttccgcga atatcgccac cactttttca cgcacttgcg gattttcacg cagaattttc
      961 aggttatcga ggcaatgctg acgattaatt cccagtcggt cggcatcttc cgggcgtagc
     1021 gtattcgcct gggccagcac cggacattta ttgatgtgca ccagcttaac cggaacggcg
     1081 gcgttatcgc caagatcggt ttttgcggta tataaacgct cgcgcaatgt gtcgctatcc
     1141 agttccagta atggcgaaat gtctcctgcc aaatccacca taattacagc attgcgattt
     1201 tcaggatgcc acgccagcgg tgccacccag ctggtattgc cgctccatgc cccaaacatt
     1261 ccggaaacgt gaaccagggg gggcatctgc ggaacatcaa tcaacgccat cagtttgtgt
     1321 ttattacgat gggtaaagag ataatcaaac aggcgtggct gacgcgtttt taccagtttc
     1381 gccatcgcaa tagtggcgta cacatcagcc atcgcatcgt gggcgttgct atgttcaata
     1441 ccattcgctt tggttaaatg ctcaaggcga aagctcggta gaccgtcatc attttcaggc
     1501 cagtttattc cttccgggcg cagggcataa caggcacgca taacatccaa caaatcccag
     1561 cgcgagttat catgctgcca gctccaggcg taaggatcgt agaaattacg ataaaaaacg
     1621 ttgcgtgtga cttcgtcgtc gaaacgcaca ttgttgtagc ccagaataca ggtcttcggt
     1681 acggtaaaaa gcgagtgaat acgggcggca aacgcggctt cgttttctcc tttcgcccgt
     1741 gcttcctgcg gggtaatacc ggtaattaat actgctccag gctg
//
LOCUS       DAJTSL010000300         1704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTSL010000300 DAJTSL010000000
VERSION     DAJTSL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.303"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(855..1328)
                     /locus_tag="OO433_004157"
                     /pseudo
     CDS             complement(855..1328)
                     /locus_tag="OO433_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1256..1622)
                     /locus_tag="OO433_004158"
                     /pseudo
     CDS             complement(1256..1622)
                     /locus_tag="OO433_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          379 a          475 c          413 g          437 t
ORIGIN      
        1 tcgtcaaaaa aaatcatctt cgtgaagttt tacgacatag tggaggagtt tgcttctttg
       61 aggtgcaggt tttcgtttgc gtggtttagc tgaacgggcc atataactac ctgaaagaca
      121 atgatattgt ctgtttttat aacggtaatc acaggccatg acaagccgca gccgtcaggc
      181 cgcctactcg gggcggttta gaacgcttat ttagtaccag atcttactta taataaaggg
      241 gcttggcagt ttggataaac tattgtcctt attccactaa tacaaacttt ttgtaaagta
      301 tattaaatat acttcatttc atactgaaat ccccggaagt attcaatcaa acaattacac
      361 cataaaagga agagggggcc ccaattaagt tgtcaagcat gtttactgcg caatcgcttg
      421 gtgctccgtc cctgtacccc gcattggtac tctggcgctc atggggcttc cacttgcgct
      481 tctcccttca catcggggtg acaggtccac gttccccaca agagcctgaa tcgacttcac
      541 gccgctttta tgccggatgc cgcgcaggca gtaaacatat cccacaggaa cgactccacc
      601 attgttttga catcatctat atgctttcga cacatcatca gcggttcact ttcgttcgtc
      661 tcttcgaacc atacctgatc ggctcacgtc cgaccttttc ccatctcgct caccaccaca
      721 tctcttaaat gcagcagcag tggatggttt ggtgcctgcg cctgcacgcc gacaccgagg
      781 ggccttccct catctcttgt gccgcatggc tgcacaggga ctcacgtccc caggacgcgc
      841 cttcgtggca cacagtcatc ggcatagcaa cagtaagcaa cggcaggttt ccattgccag
      901 ttttctttga cagcagtact tcggcctcgc tggatactgt gattccagta ccagcgatcc
      961 tttctggctt ttccactcag gtagcgttta ttcagatact ggtcgaactc attcagtatg
     1021 atgttcgaca gcaacggcga gatcacaccg ccctgtggca tactttcact gaccgcccgg
     1081 aagagaccaa catcaacacg gcgttttcca cagcagtctc ataaaacgtg catcactgat
     1141 cctgcggcag acggctttca tcagcagccg gtgatgtact gtgtcgaagt agctggacag
     1201 gtcaccttca ataacccggc gccccgtgtt tccccgcagt cggtgagctg taatagtacg
     1261 tgatgtctcc cgcccacttc tggttcgggc cactggcgta aaaatcctgc gtcagcagat
     1321 tttctgatac aggcaggccg tgtgcacggt aactgatcgg gctgaacttc tgggaggctt
     1381 ttgcgctcag ttcctgacgg cgcagactgg ctgccacggt ttttacgtta aagtggtaac
     1441 cagagcacac agttcatccg tcaggcgtgg ggcaccgtaa cgctggactg ggtaaaagcc
     1501 ttgcggacaa cgctgtcgca gtgctgacgg aactgctgac gtgtgtttat ccttgtccgc
     1561 cgctgacacc acgtatacca gccgctgcgg gccacccgga gtacgcggca cattgccttg
     1621 atgacggttt acgcggtcgc ctgcggcaac ggctttccgg ctgacggtag tgtggacctt
     1681 tttaccccgg agaacaccgg caag
//
LOCUS       DAJTSL010000301         1645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTSL010000301 DAJTSL010000000
VERSION     DAJTSL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.304"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..497
                     /locus_tag="OO433_004159"
     CDS             <1..497
                     /locus_tag="OO433_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HCR6052409.1"
                     /translation="SDRIVVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATV
                     IERLDEQRVRANVEGRECNIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGY
                     VRERNYKGMTLESVVELENGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLAD
                     EEHK"
     gene            481..1344
                     /gene="potB"
                     /locus_tag="OO433_004160"
     CDS             481..1344
                     /gene="potB"
                     /locus_tag="OO433_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6052410.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVLTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
     gene            complement(1576..>1645)
                     /locus_tag="OO433_004161"
     CDS             complement(1576..>1645)
                     /locus_tag="OO433_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6052411.1"
                     /translation="VLLNRVDTSTAPDIEWPTSPAE"
BASE COUNT          379 a          386 c          423 g          457 t
ORIGIN      
        1 tgtcagacag gattgtggtg atgcgcgagg gtcgcattga gcaagacggc acgccgcgtg
       61 aaatctacga agagccgaaa aacctgtttg ttgccggctt cattggcgaa attaatatgt
      121 ttaacgccac ggtgatcgag cgactcgacg agcagcgcgt acgcgccaac gttgaaggcc
      181 gcgaatgtaa tatctacgtt aacttcgccg ttgaaccggg gcaaaaactg catgttctgc
      241 tgcgcccgga agacttacgt gttgaagaga ttaacgacga caaccacgct gaagggctga
      301 ttggttacgt tcgtgagcgt aactacaaag gcatgacgct ggagtcggtt gttgaactgg
      361 aaaatggcaa gatggtgatg gtcagcgaat tcttcaatga agacgatcct gactttgacc
      421 actctctcga ccaaaaaatg gccattaatt gggtagaaag ctgggaggtc gtactggctg
      481 atgaagaaca caagtaagtt ccagaatgta gtgattgtca ctattgtcgg ttggcttgtg
      541 ttgtttgtct ttctgcccaa cctgatgatc attggcacca gctttttgac ccgcgacgac
      601 gccagtttcg tcaaaatggt cttgacgctg gataactaca cgcgtctgct cgatccgctc
      661 tattttgaag tgctattgca ctcgctgaat atggcgctga tcgccaccct cgcctgcctg
      721 gtgctgggct atccatttgc ctggtttctg gcgaagctgc cacacaaggt gcgtccgctg
      781 ctgctgtttc tgctgattgt tccgttctgg accaactcat tgattcgtat ctacgggctg
      841 aaaattttcc tcagcaccaa aggctatctc aacgagtttt tgctctggct gggcgttatc
      901 gacacaccaa tccgtatcat gttcacgccc agtgcggtga ttatcggtct ggtttacatt
      961 ctgctgccgt ttatggtgat gccgctgtac tccagtatcg aaaaactgga taagccgttg
     1021 cttgaggcgg cgcgcgatct cggtgccagc aagttacaga cttttatccg catcattatt
     1081 ccactgacga tgccggggat tattgccgga tgtctgctgg tgatgctgcc agcgatgggc
     1141 ctgttctatg tgtccgacct gatgggcggt gcgaaaaacc tgctgatcgg taacgtcatc
     1201 aaggtacagt tcctcaatat tcgtgactgg ccgtttggtg cagccaccag cattacgctg
     1261 actatcgtaa tgggcctgat gttgctggtt tactggcgcg cttctcgttt gctgaataag
     1321 aaggtgagcg aattagatga ttaatcattt atattcaatg cattaagttt gtttttgagt
     1381 ggttttgccg tttaaggtaa cggcgttata gatcctatca aaatcaatca attccattct
     1441 ggttttggag aaagattttt cgagagcttc ggtctttaac taaaactgat tgcgtataac
     1501 cccaataccc ctccccgact atctgaatgg tcggggaggc acttcacgca caaaatatca
     1561 tccctgcgcc gttgattact ctgcaggtga agtcggccac tcaatatcag gtgcagttga
     1621 tgtatcaaca cggttcagca acacc
//
LOCUS       DAJTSL010000302         1620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTSL010000302 DAJTSL010000000
VERSION     DAJTSL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.305"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..1613)
                     /locus_tag="OO433_004162"
                     /pseudo
     CDS             complement(1..1613)
                     /locus_tag="OO433_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441428.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          420 a          415 c          349 g          436 t
ORIGIN      
        1 cccccacaaa aaggcaaaca gatccgaagc gggataatct ttttggggag aattgttcca
       61 gttagcaata tatcccgaca gggggttata caccttaggg ttcatttcaa aaggcaatag
      121 ccctttccag tcccattttc ccgtaccagg aacgggtaat cgcggatcat ggcctgattg
      181 acgatctgga taagcaccag tatgaacata accaatattg ccgtttacat cagcatagta
      241 ccagttgatg gtcagtgctt gtttcgctac tcctgccaat ttttggcctt catctgatga
      301 gtccaggcca gcaaagacgc cacctcttta ccatcccatg cgcgggattt agcgtaagcc
      361 gtttgtgtcg tctggtcagt ttggagaatg ttgccatgca ccgtacgcca gacagtaaag
      421 gtctctgcct gaccattttt caccgtaatg gtttcctcac ggcttaacat tttcacccac
      481 ttacaattat gtaagtagta gcctggtttc tctgccgaca gccgttcagc aaaaatatcg
      541 acatcatcgc cgaaacctgc cgttgatccc caggaaatca caccattatg accaaaaacc
      601 agcccaggat aggcaaatgg tgtattgcca gtgacatcat aaccagcacc gtgcagacca
      661 ataccataag tatacgcagg cgcataccag ccaaactgcg gaccatttac catgattgct
      721 ttcgcatcct gggctttgct tttgccgatc acccacatat tgctggtcgt tggatacccc
      781 gccagaccat tggcaccacc ctgtgcaaat tgtgcagcaa tagtttcccg gttcttccct
      841 gctgttaacg ccagcagtgc gccatccgcc ccttttgctg gtcggtcaag cattggtgca
      901 ggtaaatcgt agcgtggcaa cagagctgct gtttgcgagt tttgctgatt aaatttaagt
      961 gggtagctac tctcttgtac ggcaatagtg gttggcgctg atgggtttac cagccatttc
     1021 aactgattaa ataccgccat gccttgtgat acaccatatt tatcttttaa agccgttagc
     1081 agtgccagat tatcaatttc gctagtgcta tcagagaagc ggtttgccat ggtgcccaca
     1141 aatatcatcg cgacatcaaa cggttcccag cgcttaggag taaagccaaa tgtattaaac
     1201 tgttttggta agagcgtctc tggattggta tttaccttat caatccaggc attcattcca
     1261 tcagcgtagc cttgcagaat ggacatatcc tctggggaaa gggcatcaat ttgcgcccgg
     1321 atagcatccg gccagtagtt acgacggata tctttatcaa atttcacaaa atctttgcca
     1381 agcacttccg cgacagtccc ttgagtactg cgacgtgcca tttccatctg aaaaaggcga
     1441 tcttgtgcta ctacatagcc atagccataa aataggtgcc atgtatcatt ggcataaata
     1501 tgcggcatgc cgtattcatc gcgaacaatc tttatctcac ttgacgactg ctcagccagt
     1561 gcaggtaagc tccaataata catcagggaa gcagtaacac agttcacgat catacgattt
//
LOCUS       DAJTSL010000303         1602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTSL010000303 DAJTSL010000000
VERSION     DAJTSL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.306"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1556
                     /locus_tag="OO433_004163"
     CDS             <1..1556
                     /locus_tag="OO433_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCR6052412.1"
                     /translation="MSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDL
                     CTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKIS
                     KDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWENL
                     HALKEKRLNGMPKTTPKSGQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSII
                     PPLKNYNERLHNTNLFNPKYQKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLSAIDFS
                     LLMKTIKAFSFGGDLQTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQ
                     QDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQL
                     YNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEG
                     TWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALLLGKT
                     GEPPNYLYL"
BASE COUNT          550 a          303 c          309 g          440 t
ORIGIN      
        1 atatgtctga gtttgcacgt tgccattatg ggtatataca aatccctcca gtgacaactt
       61 tccgcgccga cggaccagaa actcccgaag aagaaaaagg ttactggttt catgcttatc
      121 aacccgaaga tctttgtacc atccataacc caatgggaga tttgcaggat tttatcgcat
      181 tggttaaaga tgctaaaaaa tttggtatcg atatcattcc tgattataca tttaacttta
      241 tgggaatcgg gggtagtggt aaaaatgacc tggattatcc ctctgctgat atacgagcga
      301 agatcagtaa agatatagaa agcggtatcc ctggctattg gcaaggtcag gttttgattc
      361 cattcattaa agatccagta acaaaagaac gtaaacaaat ccatccagaa gatatacatc
      421 tcactgcaaa agacttcgaa gcaagtaaag ataacatctc taaggatgaa tgggaaaacc
      481 tccatgcatt aaaagaaaag cgtttaaatg gaatgcctaa aacaacaccc aaaagtggcc
      541 aggttattat gttgcaaaat caatacgttc gagaaatgcg aaaatatggc gtacgaggtt
      601 tacgttatga tgcggcaaaa cactcaaaac atgaacaaat agagaggtcg ataatcccac
      661 cgcttaaaaa ttataatgag cggttacaca atactaattt atttaaccca aaatatcaaa
      721 aaaaagccgt tatgaattac atggaatacc tggtaacttg tcagttggat gaacaacaaa
      781 tgtcatcact gctttatgag agagatgatt taagtgccat tgatttttct ttactcatga
      841 aaacgataaa agccttttca tttggtggag atctccaaac ccttgcatca aaaccgggtt
      901 ccacaatctc aagtatccca tcagaaagac ggatattgat taacattaac cacgattttc
      961 ctaacaatgg taatcttttc aatgactttc tatttaacca tcaacaagat gaacaattag
     1021 caatggcata tatggccgct ctcccgttca gcagaccttt agtttactgg gatggccaag
     1081 tattaaaatc aacgactgaa attaaaaatt atgatgggtc cacgcgtgtc ggcggtgagg
     1141 cgtggcttaa taaaggttgc tctacctatc agcagctcta caatgaattc cacgcattat
     1201 atatagataa agcaggaata tggagcgcat ttgagggtgt atttgcaact aaaaacgttc
     1261 tggcctttag tcgtggggat tctgtgaaca ttaatcactc tcctcatgat gggctagtta
     1321 taataaataa aggaaacgaa gaagttgaag gtacctggcc taacaaattg caacctggaa
     1381 tatacaaaaa catggggagt aatagcgtta acattattat taataatacc cgaaaaatta
     1441 tcccccctgg taaagtattt acgcttagag gcggaactct aaatatcaat attcctgggc
     1501 gtagcgctct tcttttaggg aaaacaggag aaccgccgaa ctatctctat ttataattta
     1561 atgttatatc tgccccgata aaacggggca gataatatgt tt
//
LOCUS       DAJTSL010000304         1595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTSL010000304 DAJTSL010000000
VERSION     DAJTSL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.307"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..792)
                     /gene="ospC2"
                     /locus_tag="OO433_004164"
     CDS             complement(<1..792)
                     /gene="ospC2"
                     /locus_tag="OO433_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6052413.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYV"
     gene            1217..1595
                     /locus_tag="OO433_004165"
                     /pseudo
     CDS             1217..1595
                     /locus_tag="OO433_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          488 a          286 c          309 g          512 t
ORIGIN      
        1 cacatacctg ctaacttcat ccacaacctc tttaaagtta tctaagaaaa atgattggtg
       61 ctcatggtaa aaaacaggtg gaatagcatt atcaaagtgg tcggttaatg tcatatatcc
      121 atatggagag tcatgatcaa tgatatacgc attagcacca aaatccacag tatgatgtgt
      181 tttatttagt gggagtgttt cttgatgatt ggaaatttca accccaaaaa aaacaaaatc
      241 gtggttagct atttcttcaa tatcctcaga gaatgtgttt ttatatgttt gtatattctc
      301 agacttaatt ttatcaaagg acttaatatt aagagtatta tttgacaggt tagcgttact
      361 ttgatgtctg aagttaaaat cagaattaat aataatagag agcaagtgac gttcatccga
      421 agaaagagga tgagtttttt gtattatttc agaaagatag tgtctggaca ccttatcgag
      481 gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc gcttcaatag aaagatttaa
      541 tgatttgaaa ctggtccctt gagagaacat tttactataa ctttgcgctg ctatagactt
      601 ccttaaaaag cttttcacag tatcagcaca gtgatctaaa tgtttttcac ttatgccaga
      661 agaggatgag tttgttacgc atgatatgtt tttttgtaat gcttttgtat ctttgttcgg
      721 atttattttt ccatcaacaa gatcaatatt gttctgaaca ttaatatgat ttactgcttc
      781 aggtattttc ataaaacatc ccccattatg tgcgttttta tctgcagtta tttagaataa
      841 tattatctaa cttaatattt tcttgtatgt attataacga acatatttta atatgttgta
      901 cgattttcac tctgaaatcc cctgagttta atcacacttc ctttactgaa aggaattatt
      961 gcaacagtat tatttgtttt tggatatata tcttgcgcct tgttatgtag ttaatgctca
     1021 gaatatgata gataaaatag agacgcagaa cagggcatgc tcataacatg atgataacat
     1081 aaaaaattag taacaataaa tgaaagaatc atgggcgctc aacaaaaaga gagctaaata
     1141 gctgcgcgga atagtaggct gtgtccctta atcatctgag ttatagtaac tgtctgttta
     1201 cacaacatat tgaactatgg ctcgctacga ccttcccgat gaggcatgga ccatcatcaa
     1261 gcccctgtta cctcctgaac ccgccacacc acgggccgga cgcccatggg ctgagcaccg
     1321 taaaatcatc aatggcatgt tctgggtgtt atgttccggt gcaccatggc gcgatttacc
     1381 cgaacgatat ggggcatgga aaactgttta taaccgcttt aaccgatggt caaagtcagg
     1441 cgtgattaat attattttca acaggttgct ttcgctactt gatgcaaacg gctttattga
     1501 ctggtctgcc accgcgctgg atggcagtaa tatccgggcg ctgaaatgtg ccgccggtgc
     1561 tcaaaaaaac atcccgatat cgacggagat aatgg
//
LOCUS       DAJTSL010000305         1546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTSL010000305 DAJTSL010000000
VERSION     DAJTSL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.308"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(918..1394)
                     /locus_tag="OO433_004166"
     CDS             complement(918..1394)
                     /locus_tag="OO433_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6052414.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          352 a          413 c          383 g          398 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc catgattttg cccgatttcg
      181 cgctctccac gttcaacagc tcgatcatgt ttttgccgtc cgttcaccgt taaaacgcct
      241 cagaaatcgg gacaggatgt gggaatgtcg gaccgcgcac gcgcactcag acattcccca
      301 cctggtcagg gcttcacccc gacaccctgc atccgtgcgt aagtgctcaa tgtaacttcc
      361 ggttctcttc cgcttcctcg tcgattcgct cgctacgctc ggtcatcgac ttgcggcgag
      421 cgggatcacc tctcgcatgt tgtcggtgat cccgttgttg ttctttcggg gatgagtcgt
      481 cagatagccc ttcaaacacg ctcagaagct ctcagacgca cgaaacgggt ttaaatgccc
      541 gccagggcat ggaaggcgcg aaaaacgctt acagaggctc tcaggaggtc aggcgcgccg
      601 atacccctaa ccaggggctt tcaggtcgct aggaaccgcg tcagctccct gcaaagccta
      661 tttgcacggt acaggcagct attcggctgg ttggtggtcg tagaccgtcg gtgttacagc
      721 gatgggggtc tggggttctc cccagacggt ttccggttgc gctcaaggcg ctgccggaac
      781 cgtggcgacg cggtcgttct tgggatcggg gagcaaaaac acgcaaggga ttttgaggcg
      841 gcaaaagagc aatgcaggat agacaatcat ggtcaaaacg ttaacacgta gcccctgatt
      901 gtccgcctgc cgctggctca atttgaaaac atggccatga ttcggcgctg aatatcaccg
      961 tctttgttga ccagtgtttt agtgacaaac gctgcatggt ttttcgtagt gttgtatccc
     1021 ttccagactt catgtggatc gagggcatac gcacaaacct taccagcaac ggggattcta
     1081 actaatgcat tttttgattc taagtaattg agccaccgaa taattgtgct tctgctaact
     1141 ccaagttttt cagctaggaa ttgctgatcc gcaacgacag cgccacacgt aggatcgata
     1201 tgctccgcta aaaatgagta cagcttcagt gctgatatgc cttggctatc cttcagcagc
     1261 tctcgaacgc gttcccaacc ttttggggat acctgtgtaa accttgaatt cttctttgct
     1321 tcttcgagat cctcggtgtt tttctttgcc tcatctgaaa gttgtttcag tcgtttttct
     1381 gaatcaataa acatgatttt ttccctctga aaatcccatg cactaacgtt ggttcaaatc
     1441 taagccatca tgacacatct gtgcaacacc ttgttgcgtg ttgtcgcatc caggatgcga
     1501 caacgttgtg tcaccctgga tgcgacaggt aaacaaaaaa cacact
//
LOCUS       DAJTSL010000306         1536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTSL010000306 DAJTSL010000000
VERSION     DAJTSL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.309"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..1021
                     /locus_tag="OO433_004167"
                     /pseudo
     CDS             1..1021
                     /locus_tag="OO433_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            1308..1433
                     /locus_tag="OO433_004168"
     CDS             1308..1433
                     /locus_tag="OO433_004168"
                     /inference="COORDINATES: protein motif:HMM:NF026861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052415.1"
                     /translation="MACASVRKQINNQCFGGGDKAHMEEENKAYKTAADCSGLIK"
BASE COUNT          414 a          379 c          440 g          303 t
ORIGIN      
        1 taaccgcctg cccgacccgg agctgcaccc ggacagcacc ctcagcatgt ggccggataa
       61 ccgtatcgcc cgtgacgcgc actatcttta ccggtatgac cgtcacggca ggctgacaga
      121 gaaaaccgac ctcatcccgg aagggggtat ccgcacggat gatgagcgca cccaccggta
      181 ccattacgac agtcagcacc ggctggtgca ctacacgcgg acacaatatg cagagccgct
      241 ggtcgaaagt cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtgtggcg
      301 gcgtgaacgt gacctgacgg gctggatgtc gctgtcacgg aaaccggaag tgacctggta
      361 cggctgggac ggcgaccggc tgaccacaat acagaacgac aggagccgca tccagacgat
      421 ttatcagccg gggagcttca cgccactcat cagagttgaa accgccaccg gtgagctggc
      481 gaaaacgcag cgccgcagcc tggcggatac ccttcagcag tccggcggcg aagacggtgg
      541 cagtgtggtg ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct
      601 ggctgaccgg gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtggc
      661 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaatcc acctgtacaa
      721 ctgcgaccat cgcggcctgc cgctggcgct tatcagcacg gaaggggcaa cagaatggtg
      781 cgcagaatac gatgagtggg gcaacctgct gaatgaagag aacccgcacc agctgcagca
      841 gcttatccgt ttgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      901 ccgctattat gacccgctgc aggggcgata tatcactcag gacccaattg ggctgaaggg
      961 gggatggaat ttttatcagt atccgttgaa tccgatctca gagatagacc cacaagggtt
     1021 aaatccgtta attcttggtg ttgtagcact gatgagttgc ggtttgtttt catcaggtgg
     1081 tgttatccaa agtgggcaac aacgcagaga gcaatatgga cgtaaagtta atccagaagc
     1141 cgacaaatta acagatgaac ataagtcagg tgtaaatcct ggaggtaact gtagtcccca
     1201 gaacctgagt gacttacaaa atgaaaaaaa cagattatgt aaccagtcta gagcttgcgc
     1261 tcctcagatg ctgagaaaag aaataattga gacggtacga gataaatttg gcgtgcgctt
     1321 cggtcagaaa gcagattaat aatcagtgct ttggtggtgg agataaggcc catatggaag
     1381 aagagaataa agcatataaa acagcagctg attgcagtgg cttaataaaa tgaaaaaaat
     1441 aattttctct aacgagagtg cagtctatga tgaacttatg attcatttcc catgccagcc
     1501 attacctcat atttcaaatg atattatagg gctgga
//
LOCUS       DAJTSL010000307         1470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTSL010000307 DAJTSL010000000
VERSION     DAJTSL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.311"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..695)
                     /locus_tag="OO433_004169"
     CDS             complement(<1..695)
                     /locus_tag="OO433_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6052416.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
     gene            complement(1011..>1470)
                     /locus_tag="OO433_004170"
     CDS             complement(1011..>1470)
                     /locus_tag="OO433_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6052417.1"
                     /translation="VPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFETYPAA
                     LHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQ
                     KLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          427 a          261 c          309 g          473 t
ORIGIN      
        1 tgggcggttt gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga
       61 ttgacaacgc tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc
      121 tgattaagcc agaatattct tataatatga tcatctcttt ctggaaaatc tggtattact
      181 tccaaagcat ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca
      241 gacaggtgat tgtcacttac gttaatatat tctagtgacg caggtaattc aggaagtgcg
      301 aacaaatggt tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca
      361 gataattgat tactgcttgc attcagttct ttcaatgccc ttggtagctc ggggagcatt
      421 gatagttggt tattgcttac attaatttca tcaagattgt caggcaatcg tggtagagat
      481 ctgagaccta aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc
      541 cgggtaaaag cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat
      601 tcggagagat aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt
      661 ccagtggata gggggggatt atttacagga agcataataa cctcgcagaa ggatatcctg
      721 ataaaatgtg atactgggaa ataataagct aaaataaaat gtttcaggat tgaaatttat
      781 tgttttcatt ttaaggaagt gatgttgatt taactgtaaa aaaacgatac tattttctta
      841 gatggtaaat gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt
      901 catatttgaa tgttctgccg gaatgcatta tcaatagagg taaagtcgca accccaaatc
      961 gtaaaggaaa ccgtagcacg tcttaagcaa gaacgtgctg cggttggatg ctattttttc
     1021 cctgaagcag aaaacattac tacagtacct tgaaccttgg ttttaacatt ctcgaaatgc
     1081 tctgagagta tatgtgttaa gccttcttcg gaatcttttg tgtttgaaaa gatgcctttc
     1141 tgattgtaaa tgcgcatcag tttttgaccg aagctattgt gcacaactcc atcgccaaga
     1201 attgtggctc cgtatagagt tccatcatca gttaaggcct gcgccgcatt gcgtattaca
     1261 cagctttttg tagatatatt tccaggcagg cagtgaagaa ggtaaaacat ggaaatggaa
     1321 tcaaattgac catgtaacgc cgcgggataa gtttcaaaaa catcatggct aattttatgt
     1381 ttaatttttg attccccagc ccttgtagat gccgcgttca ggctagcttc gttcaaatcc
     1441 attaaagata tcagactact ctcaggtacg
//
LOCUS       DAJTSL010000308         1467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTSL010000308 DAJTSL010000000
VERSION     DAJTSL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.312"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..769)
                     /gene="corA"
                     /locus_tag="OO433_004171"
     CDS             complement(<1..769)
                     /gene="corA"
                     /locus_tag="OO433_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6052418.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRII"
     gene            1139..>1467
                     /locus_tag="OO433_004172"
     CDS             1139..>1467
                     /locus_tag="OO433_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052419.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFSVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
BASE COUNT          373 a          379 c          366 g          349 t
ORIGIN      
        1 tgatgatgcg gttctgctcg atgttgataa aacccatcgc cgcctgcatc aggaagttca
       61 ccttctggaa cagagattcg ttatgcggca gcagggattc gatatctcgc aggatttcac
      121 gcgcctgctc cagttgtcca cccggtaagc gtgctttacg taccaggaag ttgagcgcgc
      181 gctgggtatc catcagacac aggcgtactt tccagccgat atcttccagt tccgccagcg
      241 tggagagcgc ctcatcgtac tcatcgccct gatgcccttc cataatcacc cggctcaact
      301 gctccaggtc gctatagata ttttcaattt catctgccaa ctgttcgatt ttggtttcga
      361 acagatccag cagcagctcg taggcgttac cgtctaccat cgactggcta cgggcacgca
      421 tacgatacag acgaaaagcg ggcagttcac gctcacgcag agtaaacaga cgaccatcac
      481 ggatggtaaa tgccacagtg gagttaccgg cgtgatcttc cgcatcttca aagaagaaga
      541 aggagtgaat atgcaggccg tcgtcgtctt caaagaaacg tgccgatgct tcgatgtctt
      601 ccagttccgg gcgggttgcc aggctctggc caagttcaga ttgtacacgc agtcgctcgt
      661 cgtcgtccgg ttcgacaaga tcaatccata ctgcatttac aaggggttgt gactcttcga
      721 cttccagccg ggtcagtcgg ttattttcca gttgaaatgc gctcagcatg accgggactc
      781 ccaatgctta aaatatcgga cagttcggtg ggcacacaga aacaaattgg gtttcagacc
      841 attaaacagc ctgactcagc gcgacgggaa aaattgaggt cgctgacaac cgctaaggct
      901 atcagcaaaa agggatagcc ttaggagttg atcctggatg acaggatagt gagccagtat
      961 ctactgggtg tgtccaaggc gaatgtcctc ttagaatgat cgtgcgcgca tgttacgcca
     1021 gcaaaatttt gccgtcaaca cgcaacagaa caccacagaa caatagttga acttcacgct
     1081 ttttggtact cctcaaggtc gctaacagga taaaaaaatc ccacatccag gacctataat
     1141 ggcgcatcgg ctactcattg gtaaaggaat gatcaccttg aatctcaaac ggatttttct
     1201 caccctcacc ttacttccct tgttttctgt tgccgctgat gattgcgcac tctcagatcc
     1261 gacgctgacc gtacaggcgt ataccgttaa tcctcaaaca gagcgggtga aaatgtactg
     1321 gcaaaaagcc aatggcgaag cgtggggaac gttacatgct ctgctggcgg atattaatag
     1381 tcagggtcag gtgcagatgg cgatgaacgg cggcatctat gatgaaagct atgcgccgct
     1441 cggtttgtac atcgaaaacg gtcagca
//
LOCUS       DAJTSL010000309         1442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTSL010000309 DAJTSL010000000
VERSION     DAJTSL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.313"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(28..900)
                     /locus_tag="OO433_004173"
                     /pseudo
     CDS             complement(28..900)
                     /locus_tag="OO433_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(914..1054)
                     /locus_tag="OO433_004174"
     CDS             complement(914..1054)
                     /locus_tag="OO433_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052420.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVSVFNDNESKITDAAVNLFI
                     QI"
     gene            1193..>1442
                     /locus_tag="OO433_004175"
     CDS             1193..>1442
                     /locus_tag="OO433_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6052421.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQG"
BASE COUNT          436 a          279 c          306 g          421 t
ORIGIN      
        1 tccgggatgg cattttttat ggttgagtta ccagcggccg gatgcgccgc cacctccaga
       61 agaaccgcca ccgccgctaa atccgccgcc acttgagcct ccaccggaac cgctgttgtc
      121 tgaatgataa cttttacctg atgctctttt ctgaagtaca cataaacagg taaagaccat
      181 aaatatggtg aaagtaaaaa agaaggaaaa tacagcaatg gaaaaaattt tatcggagaa
      241 caggaataaa tctaaaagat agattgctga aatacagact ccacttttac atgcgcgaca
      301 aaaattactg ccttgatgaa aaaaccagaa agggaagaac atcactgcaa atacccaaaa
      361 aatagcaaag taataatgat cactggaaga cgctgattca ctttctgaag gattagtcgg
      421 atattggtgt tgtgaagtga gttggttatt caaagcgttt atagctagct ctaatccctg
      481 agcaaatttt tgttgtttaa atgcgggtat catgttgcta cggatgatat ctccagccag
      541 agcatcggtt accttttctt ccagcccata acctacctgg atgcggacag tacgatctga
      601 ccaggcaaca atgatcagta tcccatcatt acgtttggca tctcctaaac gccaattgtc
      661 aaaaactctt gtagcatatt gttcaattgt ctcgtctttg gttgtgggaa caattaatac
      721 agcaatttgc acttgggtat tattgaaaat agattctaac ttgtgcgtca gttctttctt
      781 ttcttgtgag tttaagctac tcgtaagatc cgtaacttgt tactgtaatt gtgggacaat
      841 agttggttgg gctgcaacaa aggaacatac gaaagtgaac attagtatga aatagcgcat
      901 tataaatttc tcattaaatc tggataaata aattgacggc ggcatcggta attttagact
      961 cgttatcatt aaaaactgat acagaaaaaa taaatataat tttggtgaat gttttgacat
     1021 gattaaatcc attttcttta ttttcttctg acataatacg ttactccctt taattattaa
     1081 cgatatgttt cgcatttttc tttaaatctg catgtaacag aatgttaata aggcatgatt
     1141 gttgcggcag gatctgcaat aattaacaat tatccacttc acagagaatg ctatgcagta
     1201 cccgattaac gagatgttcc agaccctgca aggtgagggt tactttaccg gcgtccccgc
     1261 catttttatt cgtttacagg gatgcccggt tggctgtgcc tggtgcgaca ccaaacacac
     1321 ctgggaaaag cttgaggatc gggaagtctc ccttttcagc attctggcga agaccaaaga
     1381 gagtgataag tggggggcgg cgagcagtga agatttgctg gctgtcattg gtcggcaggg
     1441 at
//
LOCUS       DAJTSL010000310         1423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTSL010000310 DAJTSL010000000
VERSION     DAJTSL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.314"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..587
                     /gene="argO"
                     /locus_tag="OO433_004176"
     CDS             <1..587
                     /gene="argO"
                     /locus_tag="OO433_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6052422.1"
                     /translation="PLGPQNAFVMNQGIRRQYHIMIALLCAISDLVLICAGIFGGSAL
                     LMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAEVLKQGRWKIIATMLAVT
                     WLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWFFGLAILAAWLAPRLRTA
                     KSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            680..1420
                     /locus_tag="OO433_004177"
     CDS             680..1420
                     /locus_tag="OO433_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6052423.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
BASE COUNT          338 a          335 c          398 g          352 t
ORIGIN      
        1 tgccgctcgg cccacaaaat gcttttgtga tgaatcaggg cattcgtcgt cagtaccaca
       61 ttatgattgc cttactttgc gctatcagcg atttggtcct gatttgcgcg gggatttttg
      121 gtggtagcgc gttattgatg cagtcgccgt ggttgctggc gctggtcacc tggggcggcg
      181 tagtcttctt gctctggtat ggttttggcg cttttaaaac agcgatgagt agtaatattg
      241 agttagctag tgccgaagtc ctgaagcaag gcagatggaa aattatcgcc accatgttgg
      301 cagtgacctg gctgaatccg catgtttacc tggatacttt tgttgtactg ggcagccttg
      361 gcgggcagct tgacgtcgaa ccaaaacgtt ggtttgcgct cgggacaatt agcgcctctt
      421 tcctgtggtt ctttggtctg gctattctcg cagcctggct ggcaccgcgc ctgcgcacgg
      481 caaaatcaca gcgcattatc aatctggtag tgggatgtgt catgtggttt atagccttgc
      541 agctggcgag agacggtatt gctcatgcac aagccttgtt cagttaggcg ctatctgatg
      601 gaaaaataaa acagaggcgc taagcttgcc tccagaggtc ctgaattttg ggcaagtcaa
      661 ttaacacgga gagactaacg tgaagttcaa agttatcgcc ctagcggcat taatgggtat
      721 tagcgggatg gcagcgcagg ctaacgaatt gccggatgga ccgcatattg tcacctccgg
      781 tacggcaagt gtggatgcgg tgccagacat tgccactctt gcgattgaag ttaacgtggc
      841 cgcgaaggat gccgctacag ccaagaaaca ggcagatgag cgcgtcgcac aatacatttc
      901 cttccttgaa ctcaatcaga tcgcgaaaaa agatatcagc tcagcgaatt tacgcaccca
      961 gccagattat gattatcagg atggtaaaag tatcctgaaa ggctaccgcg ctgtgagaac
     1021 ggtggaagtc acgctccgtc agttagacaa actgaattcc ttgctggatg gcgcgctgaa
     1081 ggcgggtctt aacgaaattc gttcagtgtc gctgggcgtg gcgcagccgg atgcctataa
     1141 agacaaagcg cgtaaggcag cgattgataa cgcgattcat caggcgcagg aactggcgaa
     1201 cggctttcat cgtaaactgg ggccggtata tagcgtgcgc taccatgttt ccaactatca
     1261 gcccagccca atggtgcgga tgatgaaagc cgatgccgcg ccggtgtctg cccaggaaac
     1321 ttacgagcag gccgctattc agtttgatga tcaggtcgat gtggtcttcc agttagaacc
     1381 tgtggatcaa caacccgcta aaacacctgc agcacaataa tac
//
LOCUS       DAJTSL010000311         1366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTSL010000311 DAJTSL010000000
VERSION     DAJTSL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.315"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..488
                     /locus_tag="OO433_004178"
     CDS             <1..488
                     /locus_tag="OO433_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6052424.1"
                     /translation="SQAVADYFDKQHKHVLAKIDTLDCSPEFTSANFSADVQTVEIGN
                     GAERESRCYLITKDGFMFLVMGFTGKKAARLKEAYIEKFNAMEEELHKRPPAAQNSPA
                     PNNGCALLIHFDKHGQVEFTEKVPADAMVCTLERFKFYLEQHGWIVARKEQLVERLMR
                     F"
     gene            complement(615..785)
                     /locus_tag="OO433_004179"
     CDS             complement(615..785)
                     /locus_tag="OO433_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052425.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(900..1169)
                     /locus_tag="OO433_004180"
     CDS             complement(900..1169)
                     /locus_tag="OO433_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6052426.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            1238..>1366
                     /locus_tag="OO433_004181"
     CDS             1238..>1366
                     /locus_tag="OO433_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCR6052427.1"
                     /translation="MKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLP"
BASE COUNT          389 a          346 c          279 g          352 t
ORIGIN      
        1 cctctcaagc tgttgccgac tacttcgata aacaacataa acacgtttta gctaagattg
       61 acacactcga ctgctctccc gaatttacat cagccaactt ttcggccgat gttcagactg
      121 tcgaaattgg caacggcgca gaacgtgaat cacgatgcta cctgatcaca aaagacggct
      181 tcatgttcct cgtaatggga tttactggca aaaaagcagc cagactaaaa gaagcttaca
      241 ttgaaaaatt taacgcaatg gaagaagaac tccacaaacg tcctcctgca gcacaaaact
      301 cccccgcccc aaataatgga tgcgcattac tgatccactt cgataaacac ggtcaggtcg
      361 agttcacaga aaaagtcccc gccgatgcga tggtatgcac gctggaacgg tttaaatttt
      421 atctggagca acatgggtgg atcgttgccc gtaaagaaca attggtggaa aggttgatgc
      481 ggttttaatg aatgcaaaac cccggatgat caccggggtt aattttatcg agcgtgattg
      541 ccgctaatcg ccatcgctct gattgagcgt aaatcattca ggttattcag ttcttccttc
      601 tgctctcgct gacgtcaata aatcccatca ttgcgatcaa cctcatcctt aaccattgat
      661 gcgagcagtt cttccagttt ctgcgctgac agctttacct ggtgatcttc agcatcttcc
      721 cgggctattg tggttcgcgc agtggctgat tttgctgaca tcaccacagg gggcagacgg
      781 accattgaac catggcctcc ggaacaaata agggcaaaaa taacaaccac tatcgaaagc
      841 tcacaaacta accgcagcac gttcctgcat acgacgtgtc tgcggcataa tcccaatgat
      901 tactccctga caggatttgc aggccactca atatcaggtg cagttgatgt atcaacacga
      961 ttcaacaata cccgatattt attccatgcc tccagcaacg atctttcttc ctccgttgcg
     1021 atttccagat ctacagcatc ctgcagtggc gcaatatact cactgaattc ctggatgtag
     1081 aactgtgtgg tgacggtctt ccagccattc ggctcctgct gtatcgaagc ataccaggct
     1141 atttcaatat cgctatgctg cggcagcatt taaccccttg taattcatcg ccataattga
     1201 tttaattcac aaataaaact ataacacggt gaaatcaatg aaaaaaaaca cagatgatgg
     1261 ggctaaaatt tacacaccac ttaccctaaa gctttatgac tggtgggttt tgggagtatc
     1321 aaatcggctt gcatggggat gtcctacaaa ggaacacctt cttcca
//
LOCUS       DAJTSL010000312         1344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTSL010000312 DAJTSL010000000
VERSION     DAJTSL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.316"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..483)
                     /locus_tag="OO433_004182"
     CDS             complement(1..483)
                     /locus_tag="OO433_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6052428.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCIQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="OO433_004183"
     CDS             complement(474..776)
                     /locus_tag="OO433_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6052429.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1048..1320
                     /locus_tag="OO433_004184"
     CDS             1048..1320
                     /locus_tag="OO433_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052430.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          396 a          339 c          286 g          323 t
ORIGIN      
        1 tcagacctta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctggata caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaacacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aatgcctgaa ccacggcagc ctggcggctg caaatcgggg tgtggcagaa tatgagcttt
     1021 cagagtaacc atccagaatg agttcccatg tcacacaacc tggagcacca gaaagtccac
     1081 accagaatgg tgaaagaggt tctgaaagcc gtcgccagag ccaataatca cccttataag
     1141 tccgtatttg cagactttat caccggtcat ccctcctgca cggtctgttt ctgggaaacc
     1201 ttccacaaaa tgtacccgga ttcaccgtac gaatacgtca cgttctgcca tacctgccgt
     1261 cgttttgatt tatacgaaac agaagcggag atgaaggcgg atgacccgaa gtggtggtga
     1321 ttttcagccc cgaccgctga aacg
//
LOCUS       DAJTSL010000313         1341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTSL010000313 DAJTSL010000000
VERSION     DAJTSL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.317"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..627
                     /locus_tag="OO433_004185"
     CDS             <1..627
                     /locus_tag="OO433_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6052431.1"
                     /translation="DQKSVNVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPP
                     VSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRT
                     RIKLFYRPDGLKGNPSEAPLALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKR
                     YPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGTIEGNASL"
     gene            662..1341
                     /locus_tag="OO433_004186"
                     /pseudo
     CDS             662..1341
                     /locus_tag="OO433_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="similar to the fimbrial usher protein PapC;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
BASE COUNT          402 a          285 c          296 g          358 t
ORIGIN      
        1 gatcaaaaaa gtgtcaacgt acgtctggaa aataaaggga ataacccgtt gcttgtccag
       61 agttggttag atactggcga tgacaacgct gagcctggca gtattacagt cccttttact
      121 gccacgccgc cagtatcgcg tattgatgcc aaacgtgggc aaacaatcaa attaatgtac
      181 acagccagca cctcactgcc taaagacaga gagagcgtgt tctggtttaa cgtactggaa
      241 gttccaccaa aaccagatgc agaaaaggtc gcgaatcaaa gtctgctgca actggcattt
      301 cgcacacgta taaaactttt ctatcgcccg gatggattga agggaaatcc ctctgaagcc
      361 ccgttagccc tgaagtggtt ctggtcaggt tcagaaggta aggcgtcatt gcgagtgacc
      421 aatccaaccc cttactacgt ctcttttagc agtggtgatt tagaagctag cggtaaacgc
      481 tatccgattg atgtgaaaat gattgcacca tttagtgatg aggtcatgaa agtcactggc
      541 cttaatggca aagcaagttc tgcaaaagta catttttacg ccattaatga ttttggtggc
      601 accattgaag gtaatgccag tctgtaatca ggcaaggata taattccgca ggaagcataa
      661 cgtgactata aaaccaacta aaaattatca taatcatctg acccgtatcg ctacgttttg
      721 cgcgctgctg tattgcaata gtgctttctg tgctgaactt gttgaatatg accatacctt
      781 cctgatgggg cagaatgcct caaatattga tctcagccgg tatagtgaag gtaaccctgc
      841 tataccgggt atgtacgacg tcagtgttta tgtcaacgat caacctatta ttaaccaaag
      901 cattacattt attgaaattg aaggaaaaaa aacgcacaag cctgtatcac acttaagaat
      961 ttattgcagt ttcatataaa ttctcccgat ataaataacg aaaaagccgt tctgcttgcc
     1021 agggatgaaa cactcggcaa ttgccttaat ttgacggaaa ttatccctca ggcttctgtt
     1081 cgttatgacg ttaacgagca acgtctggat atagacgttc ctcaggcatg ggtaatgaaa
     1141 aattaccaaa actatgttga tccatcgtta tgggaaaatg gcattaatgc ggccatgttg
     1201 tcatacaacc tcaacggata tcatagtgaa actcctggtc gaagaaatga cagcatttat
     1261 gctgctttta acggtgggat gaatttaggt gcatggcgac tgcgtgcctc aggcaactac
     1321 aactggatga ccgattctgg c
//
LOCUS       DAJTSL010000314         1326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTSL010000314 DAJTSL010000000
VERSION     DAJTSL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.318"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..209)
                     /locus_tag="OO433_004187"
     CDS             complement(<1..209)
                     /locus_tag="OO433_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR6052432.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAK"
     gene            complement(350..>1326)
                     /gene="metG"
                     /locus_tag="OO433_004188"
     CDS             complement(350..>1326)
                     /gene="metG"
                     /locus_tag="OO433_004188"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HCR6052433.1"
                     /translation="DSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRN
                     AGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALADLANRY
                     VDEQAPWVVAKQESRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLNTELTW
                     DGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDPIQETI
                     TFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQALIGR
                     HTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
BASE COUNT          306 a          362 c          315 g          343 t
ORIGIN      
        1 tccttcgctt gctccacata ccccttcttc accttctccg caatcagctt aagctccgct
       61 tttgccgctg ccgcagcatc tgaaaaactt tttatctggc tttgcccttt ggtgccaact
      121 tttccccaac tgatatgcaa ctcgtttccc tgttgctcaa ctgcccagaa tttatgtgat
      181 ttttcgtcct gatagataaa gtgtctcatt ctgcatccct gtaattttat ccagttgaat
      241 gcagatgcta ccagtattta tgcgggttag agagagacaa aatcgcaaga aaacaacttt
      301 aggaatttat aaagaaaagg cgctgtcgat gcagcgcctt gaagggggat tatttcacct
      361 gatgacccgg tttagcaccg gcatccgggc ttagcaggaa aatatctttc ccgccaggac
      421 cggcagccat caccatgcct tcagagatac cgaagcgcat tttacgtggt gctaggttag
      481 ccaccataat ggtgtgacga ccaatcagtg cctgcggatc cgggtaagca gaacgaatac
      541 cggagaagac attgcgtttt tcaccgccga gatccagcgt caggcgcagc agtttgtcag
      601 aaccttcaac aaactctgcg ttttcaatca gcgccacgcg caggtcaact ttagcgaagt
      661 cgtcaaaggt gatggtttcc tgaatcggat catctgccag cgggccagtt accggcgcgg
      721 cagcggcttt tacttcttct ttagaggctt ccaccagtgc ttcaacctgc ctcatatcga
      781 tgcggttata cagcgccttg aacggattca ctttgtggcc cagcagcggt tgctggatac
      841 catcccaggt cagttccgta ttgaggaatg cttctgcacg ctcggtcagt ttcggcagta
      901 ccggcttcag gtaagtcatc agcacgcgga acaggttgat gcccattgag caaattgcct
      961 gcaggtcggc atcgcggctt tcctgtttcg ccaccaccca cggagcctgt tcatcgacat
     1021 agcggttagc cagatcagcc agcgccatga tttcgcgcac ggctttacca aattcacggc
     1081 tttcccacgc ttcaccaatc acttcagcgg catcagtgaa ggttttgtac aactgcgggt
     1141 cagccagttc gcttgccagc acgccgtcaa aacgcttgtt gataaagccc gcattacggg
     1201 aggccaggtt aaccactttg ttaacgatat cggcattcac acgctgaacg aaatcttcca
     1261 ggttgagatc gatatcatcg atgcgcgaag agagtttcgc agtgtagtag taacgcaggc
     1321 tgtctg
//
LOCUS       DAJTSL010000315         1295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTSL010000315 DAJTSL010000000
VERSION     DAJTSL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.319"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            221..>1295
                     /locus_tag="OO433_004189"
     CDS             221..>1295
                     /locus_tag="OO433_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6052434.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGAVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPGTYDRPDPRIKQLARRST
                     VTPGGAACKYNDIILADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALE
                     KNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPW
                     ICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIR
                     GIGYYGSKWRDSSATWTSQDRASH"
BASE COUNT          312 a          315 c          386 g          282 t
ORIGIN      
        1 aaaaagcccg cgctgggaga cgcgggcggc aaggaataaa caataaaacg tgaagtaata
       61 tttcagctgg cgaataatac cccatagtag tcactctgcg caactgcgcg gtctttttcg
      121 aattgcgggc tgtcgtctct cttctgccat tgtcctgtaa cttccggact tcagcccgct
      181 cctcatttta ctcacaatat tatcccggcc gggaggattc atggctttta aacactatga
      241 tgttgtcagg gcggcgtcgc cgtcagatct tgcggaaaag ctgacacata aactgaaaga
      301 gggctggcag ccgtttggta gtccggtggc cataacccct tatactctga tgcaggcgat
      361 tgcagcagaa ggtgcagtaa tcagcgccac cagcgacccg gagtattact ttgttgtggt
      421 tctggcaggg cagtcaaacg gcatgtcgta tggtgaaggc cttccgctgc cggggacata
      481 tgaccgtccg gacccgcgta ttaagcaact ggcgcgtcgc agtacggtga caccgggcgg
      541 tgcagcatgc aaatataacg acatcattct ggcggaccat tgtctgcatg atgtgcagga
      601 catgagccgt cttaaccatc cgaaagcgga cctgtcaaag gggcagtacg gaaccgtggg
      661 gcaggggctg catatcgcca aaaaactgct gccgtttata ccggcgaatg cgggcattct
      721 gctggttccg tgctgtcgtg gtggttcagc gttcaccacc ggagctgatg gcacatacag
      781 tgacgcgagt ggtgcctcgg agaattcaac ccgctggggt gtggacaagc cgctgtataa
      841 ggaccttatc ggtcgaacaa aagcggcact ggagaagaac ccgaaaaatg tgctgtttgc
      901 cgtggtgtgg atgcaggggg aatttgattt tggcggtacg ccggcaaatc atgccgcaca
      961 gtttggtgcg ctggttgata aattccgtgc agacctggcg gatatggcag gccagtgcgt
     1021 cggtggctct gctggcggtg ttccctggat atgcggggac acgacgtatt tctggaagca
     1081 gaagaacgaa tccacgtacc agacggtgta cggcagctat aaaaataaaa cggaaaagaa
     1141 tatccatttc gtaccgttca tgaccgatga gaacggggtg aatgtgccga cgaacaaacc
     1201 ggaagaagac ccggacattc ggggtatcgg ttattacggt tcgaaatggc gtgacagctc
     1261 agccacctgg acgtcacagg acagggcgag ccatt
//
LOCUS       DAJTSL010000316         1280 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTSL010000316 DAJTSL010000000
VERSION     DAJTSL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.320"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..526
                     /locus_tag="OO433_004190"
     CDS             <1..526
                     /locus_tag="OO433_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6052435.1"
                     /translation="LPWLSEADRRLQIKSALPWWLVCRGEIHKFRCVPHLTGRRFEHG
                     VTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMAVTGFYRVPLSSAPPGDI
                     LLCCFGASVPNHATIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHASAF
                     TGICNDLAAASACM"
     gene            424..1104
                     /locus_tag="OO433_004191"
     CDS             424..1104
                     /locus_tag="OO433_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6052436.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
BASE COUNT          282 a          332 c          394 g          272 t
ORIGIN      
        1 gctgccctgg ctgagcgagg ctgaccggcg gctgcagata aaaagcgcac tgccctggtg
       61 gctggtctgc cggggggaaa ttcataaatt ccgctgtgtg ccacatctga caggacggcg
      121 ctttgagcac ggggtgacgg actgttacac gctgttccgg gatgcatacc atctggcggg
      181 aattgatatg ccggattttc atcgcgagga tgactggtgg cgcaacggcc agaacctgta
      241 cctggacaat atggcggtca ccggctttta ccgggtgccc ctgtcctctg caccgccggg
      301 cgatattctg ctgtgctgct ttggtgcttc ggtaccgaac catgccacca tttactgcgg
      361 caacggtgag ctgcttcacc atctgcctga acaactgagt aaacgggaga ggtattccga
      421 aaaatggcaa cgacgaacgc attctgtctg gcgtcaccgc cactggcacg catctgcctt
      481 cacggggatt tgcaacgatt tggccgccgc ctcagcctgt atgtgaacac ggcagcggaa
      541 gccattcgcg ccctgtcgat gcagatgccg ggctttcgcc gtcagatgaa cgaaggctgg
      601 taccagatac gtattgccgg tgatgacacg gcaccggagg cggtgtatgc ccgtcttcac
      661 gaacagctgg gtgagggaac ggtcatccac attgtgccgc gactggccgg ggccggaaag
      721 ggtggactgc agattgtgtt gggggcggca gccatcgtgg gctctttctt cactgccggg
      781 gcatcaatgg cgttatgggg ttcagccctg gcagccggtg gtttttctgc caccacgatg
      841 ctgttttcac ttggagccag catgattctg ggcggtgtgg cccagatgct ggccccgaag
      901 gcaaaaacac cggattaccg cgcaacggat aacggcagac agaacacgta cttttcctcg
      961 ctggataaca tgattgccca ggggaacccg atgccggtgc cttacgggga aatgctggtt
     1021 ggctcccgcc gtatatccca ggacatcagc acccgtgatg aaggcggggg cggaaaggtc
     1081 gtggttatcg ggcgacaggg ataaaacata aaaaaatccc gcagtgatcg cggagctgcg
     1141 gggacagaca aatgaagatc aatgtgaagg agttgttttt gttactcggg caaaaaaaca
     1201 ctaacgcagc gaaattataa gcgccacagt cagtgtgtga aaatgtgaag atattcagaa
     1261 attttattcc gtcatgacgc
//
LOCUS       DAJTSL010000317         1278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTSL010000317 DAJTSL010000000
VERSION     DAJTSL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.321"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..1278)
                     /locus_tag="OO433_004192"
                     /pseudo
     CDS             complement(1..1278)
                     /locus_tag="OO433_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          331 a          341 c          271 g          335 t
ORIGIN      
        1 cttgcatctt gtatcgcagc aatggctcca aaaggtaccg gtttaccatt tgcatttaac
       61 actctgaaga gaacgcgata acccacatga gtcacaaact ccgctttaac cactgcacca
      121 cgcgtgggaa ccatcgtaac gacggtatta tcaagttcta tattcgactt cgagaaatag
      181 ttatcgctga ttgctacccg attttcatca taagggttta gctgtgtctt gaccgtatag
      241 ccgcgccagt ccgtcgcagc accgcgcata ttatctattt ctaacccggc ggccccaggt
      301 gctttaacaa gagcaatagt ttcacctaac tcctgactta acgttacccc ttcgctgtgt
      361 gccagaattc cccctctgat accataattc agcctctggg aattttggct gtagttatag
      421 ctgccgttca ccgtgccata agagccatca taggtcaggg acatattacc gccaacctca
      481 tcatcatgac tgttatagga ctgcattaaa ttccatgaaa ggttatttct ttccagcgcc
      541 gtaccgcttg atgtgagcta ccggaattat cgttttgcga aacagaatag gtggcgtaac
      601 tattacccca ccagcccgat aaaggaacag ataaagagag catgtagatc tgctcactgg
      661 tgttatcact ggcatggttg ttccccgtcc cctgtctggc agtattggaa taactccagg
      721 agacgttcca cgagagacgc ttccagctac tgctgtagcc aaattgcatt aatcgttcga
      781 catcatcggt atgccagtat tgttgccggc ttaatgtcag gtataaattg ccataatctc
      841 tccccaacga ctgcgttagc gttccctcca cgcgactacg tcggttacct gtttcccaaa
      901 agtcttcagg gctgttgcgc cgcgatgccc actcattgag ggtatagtag ccctcggtag
      961 agtaacgata gccaaccagg cgcaagctag tgtctgtgtc gtcaaatcgt tttgaataga
     1021 gaaagcgata tgactgaccc gtttctgtat catcatggct aaaattcgca cgagcatgtg
     1081 tcacatcgaa agaaattgcg cccaaagcgc caagatcttt accgacaccc agagctgcag
     1141 aaccataatt ttcagcagcc tgcatacctc catataaagt gatcccgtgg gaaaaaccat
     1201 gaagtatttg cgcctgaagg acgtccggtg taaatccgtc ttcatcgcgc aattcaccca
     1261 cgctgacatc aacatctg
//
LOCUS       DAJTSL010000318         1273 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTSL010000318 DAJTSL010000000
VERSION     DAJTSL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.322"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            95..694
                     /locus_tag="OO433_004193"
     CDS             95..694
                     /locus_tag="OO433_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6052437.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            758..>1273
                     /locus_tag="OO433_004194"
     CDS             758..>1273
                     /locus_tag="OO433_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6052438.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVVQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAENARQDATVTAEDRK
                     ATAEDVTSTGAN"
BASE COUNT          295 a          298 c          405 g          275 t
ORIGIN      
        1 gccactgcag gcatcacgat tagctgaatt ttataaccca gatacgggca ccagaaatgg
       61 tgcctttttt attgcagaaa agcgagaggt aattatgcgt aaagtttgtg cagccatttt
      121 gtccgcagcc atctgtctgg ctgtatccgg tgcgcctgca tgggcgtctg aacatcagtc
      181 cacgctgagc gccgggtatc ttcagcccca tactgatatg ccaggcagcg atgacctgaa
      241 gggcattaac gtgaaatacc gttatgagtt tacggacacg ttggggctgg tgacgtcatt
      301 cagctatgcc aacgctgaag atgagcaaaa aacgcattac agcgataccc gctggcatga
      361 ggattccgtg cgtaaccgct ggttcagcgt gatggcgggg ccgtctgtgc gcgtgaatga
      421 atggttcagc gcgtatgcga tggcgggcgt ggcttacagc cgtgtgtcga ccttctccgg
      481 ggattatctc cgcgtaactg acaacaaggg gaaaacgcac gatgtgctga ccgggagtga
      541 tgacaaccgc cacagcaaca cgtctctggc gtggggggct ggcgtgcagt ttaacccgac
      601 cgaatccgtg gccattgacc ttgcttatga aggttccggc agtggcgact ggcgcactga
      661 cggtttcatc gtgggtgtcg gttataagtt ctgattagcc aggtaacaca gtgttatgac
      721 agcccgctgg ttcaggcggg ctttttgtgg ggtgaatatg gcagtaaaga tttcaggtgt
      781 actgaaagat ggggcgggta aacctgtcgt aaattgtgcg attgaactgc gggccagaag
      841 aaccagtccg accgttgtgg cacacgttgt tgccacttgc gtgacggaca ataacggtgc
      901 ttatgtgatt gaggctgagc cggggtatta cgaggttgcg cttcactgta acggctggca
      961 gccaacccgt gtcggggata ttgatgtggc accgactgat gcaccgggga cactgaacgc
     1021 gtttctgaat gcaccaaagg atggtgattt acgtccggag gtgatgaagc gctttgagga
     1081 aatggtggtg caggcgcagc agagcgccgg ggccgcagcc ggaaacgcac agcagacggc
     1141 gcaggatgtg gcggcagccg caacggcccg tgatgatgca caacgttttg cggagaatgc
     1201 cagacaggat gcaaccgtca cagctgagga cagaaaggcc actgcggaag atgtgacaag
     1261 cacaggagca aat
//
LOCUS       DAJTSL010000319         1262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTSL010000319 DAJTSL010000000
VERSION     DAJTSL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.323"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            210..1157
                     /locus_tag="OO433_004195"
     CDS             210..1157
                     /locus_tag="OO433_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6052439.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
BASE COUNT          342 a          245 c          306 g          369 t
ORIGIN      
        1 ccgtcatgct cgtctactgt tgttctggtt gttggtgatg cggtggcgct tgcactctct
       61 gaattgaaaa aatttactcg tgctgatttc ggcttatatc atccaggagg tgcactcggc
      121 attaaagcaa attcataatt tcaaagtata aatacagtga ccggtcacgc catcttttgg
      181 ggtggcgttc attgaagtga gtgtcagcta tgaaaaatgt tctggtcacc gtccccagct
      241 tttcagcacg ctgtgtttcc gccagcaagt tgctaagaga gaataatttt aatcttatta
      301 ttaagaataa tgtggagcat ctgcttaaat cagaatccac tgcattacga gaatctattt
      361 gcgcggtcat cgccggaaaa gattgttatc aggcggacac gctatcattg ctgcctggtg
      421 tgaaaataat atcgagattc gggacgggga tagataatat tgatcttcgt gcagcacaac
      481 aatccggtat tgtagtcaat aatgcggttg ggattaattc aaatgctgtc gcggaattta
      541 tcattggact tatcttcgcg agcatgagaa atattcccgg cagctatcat gcgatgcaaa
      601 atggctactg gggtgagtcg catggctatg aattacaagg caaacggatt ggcatggttg
      661 gctacggcaa tataggtaaa accctggcaa aaaggttatc cggttttgat gttgagcttt
      721 tagcttatga caaacaaccc gattatcagg ttgctgacaa agttggagtt cagtttgtat
      781 caattgaaga tatctttatg cagtcgcatg tcatcattgt tcttttgcct ttttctcctg
      841 agctggagaa cttcattagt cataaatatt tgtcgatgat gcgcaatggt gctctaatca
      901 tcaatgccgc aaggggaaag ttgctggatg agggcgcttt actacaggtg attgaagaac
      961 gaaatgtgtt tgcggcgctg gatgtgttta gctctgaacc gttggcgcaa tttagcccac
     1021 tgctacatgc caaaaatatc attacaacgc cgcatattgc ggccgccacc gttgagtcat
     1081 accagcaaac cggtacacac gttgcccagt cgataattga ttacttcgca ggaagggaga
     1141 taaaaaacgt gttgtgagtt cagcgaacga agtttttaaa tacaaaaaaa ccgcaatttg
     1201 tggaacacat tgcggtttat cagcattcta agtattggtt gcgggggccg gatttgaacc
     1261 ga
//
LOCUS       DAJTSL010000320         1252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTSL010000320 DAJTSL010000000
VERSION     DAJTSL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.324"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            105..293
                     /locus_tag="OO433_004196"
     CDS             105..293
                     /locus_tag="OO433_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052440.1"
                     /translation="MLTRKSIDPVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            303..>1252
                     /locus_tag="OO433_004197"
     CDS             303..>1252
                     /locus_tag="OO433_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6052441.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIRVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSHITT
                     ESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFG"
BASE COUNT          282 a          337 c          373 g          260 t
ORIGIN      
        1 gacggctgca aattgaaaac cgcccctgtt cgtcctgttg ttgccgcacg ctccgcagaa
       61 tatgtatgct ctgaggacct gttttattaa ggactcatcc acttatgttg acccgcaaaa
      121 gtatagatcc cgttctgctc tctgttggtg ctgagaagct ctcacaacgt gaatgggact
      181 ggatgaagat gctcaaaccc atggacccac cacctgcgat ggtcgcggcc tccatcctgg
      241 agcgccgtgg cgacacagca gcccttaccc ggctacagga taccgggggt taatccgtcc
      301 tcatgttgtc cgcttttact ccccgaccgc tgaaacgtct gttcacagcc aaccagtgct
      361 ggacgtcctt cctggatgcg ggcggtctgc gcgatatcag ggttgaggct gtcacaaaaa
      421 tgctggcctg cggcacgcgg atactgggag tgaaggaata catctgcgat aaacctgagt
      481 gcccccacgt cagatacgtc actaactcat gcggcagccg tgcctgcccg tcctgcggaa
      541 aaaaggccac agacctgtgg atagcgacac agctgaatcg tcttcctgac tgcgactggg
      601 tacacctggt cttcaccctg ccggacacgc tgtggccggt gttcgaaagc aaccggtggc
      661 tgctgaatga cgtgtgccgt ctggcggtgg agaatctgct gtatgccgcc cggaaacggg
      721 ggcaggaacc cggtatcttc tgcgccatcc acacgtatgg ccgtcgtctc aactggcatc
      781 cgcatgtaca tgtgtctgta acctgtggag gtctgaataa gcatggtcag tggaaaaagc
      841 tgagcttcct gaaagacgcg atgcgttcac ggtggatgtg gaatatgcgg cagctgcttc
      901 tgaaagcgtg gtcagagggg ctggcgatgc cggagtcgtt gtcacatatc acgacggaat
      961 cacagtggag aagcctggtg ctgaaagccg gaggaaaata ctggcatgtg tacatgtcga
     1021 aaaaaacggc cggcgggagg aatacggcgc gctacctggg tcgttatctg aagaagccgc
     1081 cgatagcggc ctcccggctg gcacattaca acggaggggc gagcctgagc ttccgttacc
     1141 tggaccacaa aacgggagaa acggcgacgg aaacgctgac acagcgtgag ctggtcgcga
     1201 ggctgaaaca gcacatcccg gagaagtttt ttaagatggt gaggtacttc gg
//
LOCUS       DAJTSL010000321         1239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTSL010000321 DAJTSL010000000
VERSION     DAJTSL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.325"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..190)
                     /locus_tag="OO433_004198"
     CDS             complement(<1..190)
                     /locus_tag="OO433_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="mutations in this gene result in a compromised
                     ability for drug-inducible mexXY expression; expression is
                     inducible by the same ribosome-targeting agents that
                     induce mexXY; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6052442.1"
                     /translation="MGNYIRPLSDAVFTIASDDQWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSV"
     gene            complement(436..888)
                     /locus_tag="OO433_004199"
     CDS             complement(436..888)
                     /locus_tag="OO433_004199"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6052443.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(885..>1239)
                     /locus_tag="OO433_004200"
     CDS             complement(885..>1239)
                     /locus_tag="OO433_004200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6052444.1"
                     /translation="NSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVK
                     PDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGLHV
                     TLLDPQMERQLVLGL"
BASE COUNT          335 a          294 c          277 g          333 t
ORIGIN      
        1 caacagagaa gaacgctgcg ccaatcggat agccgcgtcc gggatgtaaa tctggcatcc
       61 ccactacgcg ctgcatgttg ggtaaatttg ccgtggtgtg taattgttgg atcgctaaac
      121 tctcgatcca ctggtcatca gatgcgatgg taaataccgc atcggataag ggacgaatat
      181 aattgcccat ataccattcc gtaaatagac tcgtcatact tcaagttgca tgtgttgcga
      241 cttgaattat tgagagtaac aattaaaaaa tcaggaaatg gcagacgaat ccagggcaag
      301 aaaaaaataa ttaatcctgg aaaggtctgc aaagagtcat tgataaagga tttgtcatga
      361 tttacctcct tgcagtttgt ggaataagaa aacgcgcaca tactaggctt gcgttatttt
      421 cattgcaagc tggatttaat gttgctgttt ataacgcata taaaaattaa taaaccactc
      481 tccccgacat tcaacgcgtt gctgcttaac tgtctgaaaa ccataacgtt caaaaaaggg
      541 ttttgcggtt atgcttgcgt ccaccgtaag ttcggattca gacttaatca aaggttttaa
      601 caaagcgctg gcaaccccac ggcgggtgta ttcagggtca acaaataaca tatcgatata
      661 gtgttcaacg caggtaataa agccgaccag ttttgcatta atgactgcaa cccgcacttg
      721 tgatttcgcg agtttctcct tccagcgaga ttcgtcaatc tgcgcccagg cggcaatttg
      781 ttgtggtgaa taatgctgac tggcagtcat cgtaaccgct ctaatgaaaa tagcgcatag
      841 ttgctgaaaa tcgccaggct gatagtctct tatttgtatg ttattcataa tcccagcacg
      901 agttgtcttt ccatttgcgg gtcaagcaac gtcacatgca gccccaccag tcgcacaccg
      961 cgcccgccgc ggcgttcatc ccaggtttta cgcgcggtgg cgattaagtc agctttattc
     1021 agccgcggcc agacgtgctc ctgagtggtt tgctgaaaat catcaaactt taatttcacc
     1081 ccctggcgag caatcagtaa atcaggtttc acctttgcca gacggcgttc aagttccgga
     1141 tacagccgct cgataatcgc ttcacattca gaccagtggt ggatatcttc cgccatcgtg
     1201 cgttccacgc cgacggattt tcgcaaccgt tcgctgtta
//
LOCUS       DAJTSL010000322         1235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTSL010000322 DAJTSL010000000
VERSION     DAJTSL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.326"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>1235
                     /locus_tag="OO433_004201"
     CDS             <1..>1235
                     /locus_tag="OO433_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR6052445.1"
                     /translation="PMDFNIYEIPGSVYRRAKEVVLKKESPFKEWSAALRAPPGILDY
                     SRAAIFALIRSAHPEFYHYPGRLQGYINAYLTETDHENPSKETLTATRHTPEKDILEE
                     INREVVTERETEEEKPQPSDAMAGEQATTETMEPDTTEHCQNAQSLDAQSQVSSANQV
                     KVTADEVNKIMQAANISQPDADKLLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQ
                     HESERNDQKAEQNSPNALQNEPETKQSEPVAQQEPEKVCTACGQSGGGNYPDCGAVMD
                     DATYQKTFDEENQVEVQENDPEEMEGAEHPHKENAGSAQDHASDSKTGETADPLITVN
                     GHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETA
                     GGVIDRDTIKWWLKQSREA"
BASE COUNT          396 a          301 c          308 g          230 t
ORIGIN      
        1 ccgatggatt ttaatattta tgaaattccg ggcagcgttt atcgtcgcgc aaaagaagta
       61 gtcctgaaaa aagaaagtcc gttcaaagaa tggtccgcag cacttcgtgc acccccgggt
      121 attctggact attcccgcgc cgctattttt gcacttatcc gaagcgcaca ccctgaattt
      181 tatcactacc cgggacgcct tcaggggtat atcaacgcct atttgacgga aactgatcac
      241 gagaacccca gcaaggaaac tctcacagct acccggcata cgccggaaaa agatatcctg
      301 gaagaaatta accgcgaggt ggttactgag cgtgaaacag aagaagaaaa accacaacca
      361 tctgacgcaa tggcaggtga acaggcaaca actgaaacaa tggaaccgga tacaactgaa
      421 cattgccaga acgcgcagtc gctggatgct cagtcgcagg tgagttccgc taaccaagta
      481 aaagtcaccg ctgacgaagt aaacaaaatt atgcaggcag ccaatatcag ccagcctgac
      541 gccgataagt tacttgctgt atcgcgtggt gaatttgttg aggggattag cgaccctaat
      601 gatccgaaat gggtcaaggg gatccagact cgcgattctg tgaaccagaa ccagcatgaa
      661 tcggaacgga acgaccaaaa agcggaacaa aacagcccaa atgcgttaca aaacgagcca
      721 gaaacgaaac aatccgaacc agtagcgcaa caggaaccgg aaaaagtctg caccgcctgc
      781 ggtcagagcg gtggcggcaa ctaccctgat tgtggcgcgg tgatggacga cgcaacatac
      841 cagaaaacat tcgatgaaga gaatcaggtt gaagttcagg aaaatgatcc ggaggaaatg
      901 gaaggcgctg aacatccaca caaggagaat gctggcagcg ctcaggacca cgccagcgat
      961 agtaaaactg gcgagacggc agatccctta attacggtga atggtcatca cgaaatcaca
     1021 tccaccagca ggacgtgtga ccatctaatg atcgaccttg aaaccatggg aaaaaatcct
     1081 gatgccccga tcatctcaat aggtgcaata tttttcgatc cgcaaaccgg agatatggga
     1141 cccgaattta gtaagactat cgatctggaa actgctggcg gagtcattga tcgggacacc
     1201 attaaatggt ggcttaagca atcacgcgaa gcgca
//
LOCUS       DAJTSL010000323         1199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTSL010000323 DAJTSL010000000
VERSION     DAJTSL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.327"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OO433_004202"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OO433_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6052446.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLTLDVSNNLLTSLPENIITLPICTNV"
BASE COUNT          341 a          215 c          259 g          384 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgtcaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagcctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaacaatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtc
//
LOCUS       DAJTSL010000324         1155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTSL010000324 DAJTSL010000000
VERSION     DAJTSL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.328"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..303)
                     /locus_tag="OO433_004203"
     CDS             complement(<1..303)
                     /locus_tag="OO433_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052447.1"
                     /translation="MADKAILWALISASTKEERKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVN"
     gene            complement(1011..>1155)
                     /locus_tag="OO433_004204"
     CDS             complement(1011..>1155)
                     /locus_tag="OO433_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6052448.1"
                     /translation="LSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNK
                     ENP"
BASE COUNT          337 a          239 c          196 g          383 t
ORIGIN      
        1 atttactgtt tcaatgcaat cagcggcaag ttggagtgga tttagatttt tcagatatgg
       61 tcttatgatc tttcccttac ttaataaata gcatgtatag gattcggaaa gtccagcatc
      121 tattttgtac ctcattatat tagataagga tgtgagaaat tccttgttat cattcgcagc
      181 catatatgcc agtccgagtt cagcctctgc ggccttacag gcaaaatagc ttagtgaaca
      241 cgcttttcgt tcctctttag tactggctga tattagtgcc cataatattg ctttgtctgc
      301 caaatctaac tctctctgaa attgtcttgc agtaactgca ctcgcagaaa tagagaaaaa
      361 aatacaattg ttaataacct tgccatattt ttttagtccg taactgttaa tgtgctatct
      421 ggctctgaaa atattaactc atttaatttt tgcatgtcat tagataaacc agttatacaa
      481 ccctcggaac agaatcgagg tcctaaagac ggaccaaaag cgtttaaaca actatgtatt
      541 gaaaatccat tgcgattata agttcctgta ttaactgacg gtactaatcg tatgccgtca
      601 ggttttctat tgcttgctcc cgggtcatta acattccaac tccataggcc ttgaggtatt
      661 ggccatctat ttataaatgt gtacaacggg gattattttt acattgcata ttattaccag
      721 aagcgactgg aatactaagc acatcataaa aattatcatc acatggtata tctgaaacac
      781 acgaaagcat acctaaagtt atgctgtata aacaggtggc taatcccaag gggatcgatt
      841 ccagaaatcg ggttcaacgg atactgataa aaattccacc taccttccag cccaatcgga
      901 gcctgagtgt tataccgccc ctgctgcggt taatgttacc gataatagtt atagtacagc
      961 cctgattcat catcatacta ctgctgcggc agtctgatga actattgctt ttacggattc
     1021 tccttattct tccttctatc cattcgcttt agctttttga gctcttgaag tttgctcttt
     1081 gcatcaatat taccgttatt tacagcgaat tccatccaat aaatagctct attcaaatta
     1141 taatattctg ataat
//
LOCUS       DAJTSL010000325         1152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTSL010000325 DAJTSL010000000
VERSION     DAJTSL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.329"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..263)
                     /locus_tag="OO433_004205"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="OO433_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO433_004206"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO433_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6052449.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          337 a          282 c          176 g          357 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttactcttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagtg ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcga gtctaaaaag
     1141 tgatccactt ac
//
LOCUS       DAJTSL010000326         1124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTSL010000326 DAJTSL010000000
VERSION     DAJTSL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.330"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            7..690
                     /locus_tag="OO433_004207"
     CDS             7..690
                     /locus_tag="OO433_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCR6052450.1"
                     /translation="MKTQHDHYDRLSVRLSVIISRLLAGESLELKALADEFGVSERTL
                     QRDFQQRLIHLDLEHSSGSYRLTCRTGREHSPNTFAFIRNSGISGIIPAQNRRLIRLI
                     TSNSGSSPCLIGHGGLPSPVTQTACFLELAEAIRDYRAVTLLVNGKSYEAVAPRRLIF
                     QSKNWFLVATRTRNLQVFRMEDVNAVTVLDTSFRRKPELDALTASEEFISALPHFRFI
                     SDVIQTFRE"
     gene            729..>1124
                     /locus_tag="OO433_004208"
     CDS             729..>1124
                     /locus_tag="OO433_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR6052451.1"
                     /translation="MNDPVKGEGRMITDASGVTRREGGILGMWSNIFPERNGSNEQKG
                     DSKPHIPDIRDILDIFPQPLREKLLNKISEAIDYEPVIGVMGKTGAGKSSVCNALFKG
                     EVCAVSDVEACTREVQELRIRFGKHSLKII"
BASE COUNT          301 a          267 c          278 g          278 t
ORIGIN      
        1 cccactatga aaacacagca tgatcattat gaccgcctgt ccgtcaggct atcggtcatt
       61 atcagccgct tactggctgg cgaatctctg gagcttaaag cgttagctga tgaatttggt
      121 gtctcagaac gcaccctaca gagggatttt cagcaacgcc tgatccatct ggatctcgag
      181 cattccagcg gaagctaccg acttacctgc cgaacgggcc gggagcactc acccaacaca
      241 tttgcgttca tccgtaattc cgggatttca gggattattc cggctcagaa tcgcaggtta
      301 attcgactga ttaccagtaa ctccggaagc tcaccgtgcc ttatagggca tggagggctg
      361 ccatctccag ttacacaaac cgcctgtttt ctggaactgg cggaagctat ccgtgactac
      421 cgcgccgtta cattattagt taacggtaaa agctacgaag cagtagctcc ccgccgtctg
      481 atatttcagt ccaaaaactg gtttcttgtt gctacccgca ccagaaattt acaggtgttt
      541 cggatggaag atgttaacgc cgtaaccgtt ctggatacct cttttcgcag aaagcctgaa
      601 cttgatgctc tgactgccag tgaagagttt atttcagccc tccctcactt tcgtttcata
      661 agtgacgtaa ttcagacctt cagagagtaa cagacaacgt tcatttcaaa ttaaaaagga
      721 gacctcgtat gaatgacccg gtaaagggcg aaggccggat gattactgat gccagtggcg
      781 tcaccagaag ggaaggcggg attttgggaa tgtggagcaa tatatttcca gaacgtaatg
      841 gtagcaacga gcaaaaaggt gatagcaagc cgcacatacc cgacatcagg gatattcttg
      901 atattttccc tcagccactg cgggaaaagc tgttgaacaa aatcagtgaa gccatcgact
      961 atgaacccgt gattggtgtc atgggaaaaa ctggcgcggg gaaaagcagc gtatgtaatg
     1021 cgctgtttaa aggggaggtt tgtgcagtta gcgacgttga ggcatgtact cgcgaagttc
     1081 aggaattgcg tatacgcttc ggcaaacatt cgctaaagat catt
//
LOCUS       DAJTSL010000327         1116 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTSL010000327 DAJTSL010000000
VERSION     DAJTSL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.331"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..161
                     /locus_tag="OO433_004209"
                     /pseudo
     CDS             1..161
                     /locus_tag="OO433_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            178..696
                     /locus_tag="OO433_004210"
                     /pseudo
     CDS             178..696
                     /locus_tag="OO433_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          325 a          260 c          244 g          287 t
ORIGIN      
        1 gtgacagact ggcacgtctt cgtaaggagc tgaggctgca ctgtaagcag aaacgcaagt
       61 tcagggcgac cacaaactct gaccacaatc tgccggttac gccgaatctg ctgaatcaga
      121 actttacgcc cacagcacct aatcaggtct gggtcgcaga ttcagttgtc caggccttcc
      181 gaaatcaacc gactgaaggc gctggtcgcg aaactgcagc gtatgcagtt cggtaaaagc
      241 tcagaaaaac ttcgcgcaaa aaccgaacgg cggatacagg aagcacagga gcgaatcagc
      301 gcacttcagg aggaaatggc ggaaacactg ggtgagcaat atgacccggt actgccatct
      361 tccctgcgcc agtcttcagc ccgtaaaccg ttaccagcct cacttccccg tgccccccgg
      421 gtcatccggc cggaagagga atgctgtccg gcctgtggtg gtgaactcag tccactggga
      481 tgtgatgtgt cagagcagct ggagcttatc agcagcgcct ttaaggttat cgaaaaacaa
      541 cgccctaaac tggcctgtcg ccggtgcgac catatcgtgc aggcaccggt gccttcaaaa
      601 cccattgcac gcagttatgc cggagcgggg cttctggccc atgttgttac cggaaagtat
      661 gcagaccatc tgccattata ccgccaaagt gatctactat tccacacagc tatttagctc
      721 tctttttgtt gagctcccat gattctttca tttattgtta ctaattattt tatgttatca
      781 tgatgttaga acacgctctg ctctgtgtct ctattttatc tatcatattc tgaggattaa
      841 ctacataaca aggcgcaaga tatatatcca aaaacaaata ataccgttgc aataattcct
      901 ttcagtaaag gaggtgtgat taatttcagg ggatttcaga gtgaaaattg tacaacatat
      961 tgaaatatgt tcatttcgtt ataatacaca caagaaaata ttaagttaga taatatattc
     1021 taaataacca tagataaaaa tgcacataat ggggaatatt ttatgaaaat acctgaagca
     1081 gtaaatcata ttaatgttca gaataatatt gatctt
//
LOCUS       DAJTSL010000328         1114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTSL010000328 DAJTSL010000000
VERSION     DAJTSL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.332"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            15..173
                     /locus_tag="OO433_004211"
     CDS             15..173
                     /locus_tag="OO433_004211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052452.1"
                     /translation="MYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQ
                     AYDPARSK"
     gene            173..763
                     /locus_tag="OO433_004212"
     CDS             173..763
                     /locus_tag="OO433_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR6052453.1"
                     /translation="MDSLQDDYTKLLYGLMQPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPVDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            750..>1114
                     /locus_tag="OO433_004213"
     CDS             750..>1114
                     /locus_tag="OO433_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6052454.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRFATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDT"
BASE COUNT          282 a          242 c          328 g          262 t
ORIGIN      
        1 tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc catcagtttg tctgctggtg
       61 aagtggccca ccggttaatc gtcccttcat cggatattga cctgggggag accgagcttc
      121 cggtgctcgg cgagatcacc tggcaggctt atgacccggc aaggagtaaa taatggattc
      181 gttacaggat gattatacaa agctgctgta cggactgatg cagccggggc ctgcatggtc
      241 agataccgac ggtgtacttg acggtctggc accatcgctt gtgcgtgttc accagcgggc
      301 tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca gagctgattg aacgttatga
      361 agaattgtat ggtttacctg attcctgttc ccctgtcgga acccagacat tacgccagcg
      421 tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc ataaatgagc agttcttcct
      481 ggatcagctt gaggcgctgg gatataccgg cgtaacgatc gaacagttcc agcacctgga
      541 tgcaagcccc gatccggaat ggggagatcg ctggcgttat ttctggcgtg tgacgttgcc
      601 ggtggatgcc ggtgctcagt ggcagacatg cacggacgcc tgcaacacac cgatccggac
      661 gtggggggat acggttgcgg agtgcgtgat taataaatta tgtccgtcac acaccgtcgt
      721 tttattttcc tatccagatg aggatgaaga tgcacaggat tgatacgctg actgcggtaa
      781 aagataagtt tggtccgggt aagaacggat ttactgacgg aaatcttcgc acaggacgtt
      841 ttgctacctg gctgaacagt gccatgtgga atgccattca ggaggaaatc tgtggtgtca
      901 ttgagaaggc cgggatagaa ctgaataaag aagaacacga ccagctatat aaggctatat
      961 tattgctggt gggcggtgca attaacgaag aggcattgct gataaaaaat aacctttcgg
     1021 atgtggaaga cagggatgag gctgttgaaa acctcggatt aaaacccacg gtggataagg
     1081 caaaaaatgc cgttcagcgt gatggcgaca ccat
//
LOCUS       DAJTSL010000329         1110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTSL010000329 DAJTSL010000000
VERSION     DAJTSL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.333"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(684..1100)
                     /locus_tag="OO433_004214"
     CDS             complement(684..1100)
                     /locus_tag="OO433_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052455.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
BASE COUNT          290 a          278 c          196 g          346 t
ORIGIN      
        1 agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg
       61 taagaggcta acctacatgt gttcagcatg gattgagcct cagattaatg ttaagcgtct
      121 tgcaggacgc gtagtgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg
      181 agacagatag cctcaagcac ccgcagtcat tctacttaac taagatttcc ctgcaaaccg
      241 tttttgtccg gcacagtaaa tatccaacta aaccaatagc gttcgctgta tttaccgcca
      301 gtattcaatg cacatgaccg ccatgaacac ccctaaaaaa agggcattta tatgtccaaa
      361 cattaatatc aaaacatcaa ttttttccat ataccttgct gtgaagatga tgggcataca
      421 tgatgcgaac aaccagaacg caacaaacaa aaactgcaat gcgtttttca ttattccccc
      481 tacaatcaat gtgcaataac atttaaacac acctcaattt ggccggacat ataaatatct
      541 aaaccagaaa aaatcactta catagcgtta caaactcttt agtctaaata ctcatcgtaa
      601 aacattcccc atacttatca gtccgttccg cgccaggtag cttattgcct tatctggcaa
      661 cctataatca ggtttccgct ttttcagttg gctggtcgtt taaccgacat agttaaccca
      721 ttaatctggt tgccggatgc tggtggattt tcgcgtttta gttgttcata aaagtgcaca
      781 gctttaacca gttcttctga tgtaaccggg actggtgggg cagtgaataa ggcctgaatt
      841 tcatagttcg gcctgtcgtt acaatcctct tttttcggta catatttcca gtcaccaacc
      901 cacaactccc cctgagagtc cataacacct tttttcacgt agcgatatcg ccacgctatc
      961 ggctctgctt ccagcgatgc ccgtgcaatt ttgaataact cgccctctac tcgcgccatc
     1021 cctgaattgg ggtggcattt cgtaatcgct atttttaatt tggcttcttc gattaattgt
     1081 tcttttgtta attcagtcat tttcattacc
//
LOCUS       DAJTSL010000330         1104 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTSL010000330 DAJTSL010000000
VERSION     DAJTSL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.334"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..722)
                     /gene="pdeC"
                     /locus_tag="OO433_004215"
     CDS             complement(<1..722)
                     /gene="pdeC"
                     /locus_tag="OO433_004215"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR6052456.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFSSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFY"
BASE COUNT          346 a          232 c          230 g          296 t
ORIGIN      
        1 ttataaaaat gggttataag acgttgatat gaagtcgcaa caatggctgc aattgggcgt
       61 tttgtcgaat aaactgtcgc atataaatag ccatttcttt gtacggttaa atccttcaaa
      121 tgaatcagcg gagaaaacgt tgctgccgag gcctctttgc ttaacgagaa aaaggttttc
      181 gtcaccgtat catacacacc ccattgcaat gtcggatcat cagacatcac ttcactccag
      241 aagagagggt tgataaccgc cacataattt ccccgctgca tataggtcat tttatagcca
      301 gaggaaaaag gcgtatcgcg gtaataatag atagaaacgt taggttcacg cttataatcg
      361 gccggtgcaa tcgtatagcc gtttacaggc gctatcagcg atgagcataa aaaatggtta
      421 tcacgggcat agatcaattc attaatataa agatagccac gaataatatt caacattcgc
      481 ttttgatggg ctggagtgca aacttgcccc tgatagcgtt cagctgcatc gctcactaaa
      541 tctgcctggc gaatgaccag ctcggattta tccagtgcca gttgagcaaa ggttcggagc
      601 tgattattca cttctgattt tgcccaaagg aaggcaatcc atagcgaaag aatgatggga
      661 aagagaacta aaaagataat gcccggcaac gccagtaatt ggtgtcgtgc acgatgactc
      721 attgctatca atttcctgcc tgtttatacc cggccattgt aaggtgaaaa ccctgggtac
      781 cgacgtcttt agataaatct tacggtataa atagcacttt tgcctgattt gaaattgata
      841 cctttctaat cgtcacctgg atgaaaataa ttcattgata ttcaaaatta atttcctaca
      901 ggaatgcaga ggcggcggga agatatgccc ttaatgccac tgaaaaacca cataatctgt
      961 acagcaataa aagtcacggc ctaatatggc taacaaacaa tcaattgtga tatatttcac
     1021 aaaattaatg aaacaaacag agtgtttcat ttttgtgttc tgcaagcccc gcgatagcca
     1081 gagtgtacca cgcctcccgt gaac
//
LOCUS       DAJTSL010000331         1091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTSL010000331 DAJTSL010000000
VERSION     DAJTSL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.335"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(165..788)
                     /locus_tag="OO433_004216"
     CDS             complement(165..788)
                     /locus_tag="OO433_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6052457.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
     gene            complement(739..>1091)
                     /locus_tag="OO433_004217"
     CDS             complement(739..>1091)
                     /locus_tag="OO433_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6052458.1"
                     /translation="KINITTEDIIYRIVASSVRNRWGDPDIGGLIIAAYQGEADGDKV
                     IRLVRGQSYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPGSYCALSGGPDA
                     HVSGAMPGLFIRTS"
BASE COUNT          227 a          332 c          259 g          273 t
ORIGIN      
        1 cacagaccgg actgactccg gaaaaacaga ggcccgcccc cgggcctccc cggattcatc
       61 cgtttccctg ttcagcctga cagcacgccc ccgcggccgg atgacagact ccgcttcggt
      121 aagcaaagcg gtcttctgtg attccgccag ttgcggctta ttcattactc aacgtcaaac
      181 gcccgaattg aagccaaatc atccagaccg ctcagctcct ctttcatttc acgctgacgg
      241 cgataaatct catcgttgcg atcgacctgc gcctgcacca ttgctgccgc cagttcttcc
      301 agttccggca tcgacagttt cacctgctga ttatcggcat cgctccacgc catatgtgtt
      361 tgtgctgtga cagattttgc cagcatgact accggggaca ggcggcccag tgagtcggga
      421 ccagcattcc agatacgacc gttccattca aacgtgaacg gcttcgcctc ctgttctgcg
      481 cgccatgctt caatttcctg acgtctggcc tctctggccg cttccagcat ttctggtgtc
      541 acagtgaatg gggctatctc accccatttg ccgctttgca gttcctgcca gatttgctga
      601 cccgtcggag cgacatcatc agcggtggct gtgtagggga ctgcctggtc cctgtcgtca
      661 aaaaaaacgt cacagtctac tgcgccactt tcggtataac ggggattaat gattttttta
      721 atttccacgg tgcattcctc acgatgtgcg aataaaaagc ccgggcattg cgccagagac
      781 atgtgcatcc ggccccccgg acagggcgca atatgacccc ggtaatgaat gctctgaaca
      841 tcccgtaatg aaaaattgtg gggatgctat atacgttccg gtgggagtac tgggcactga
      901 aatccccacc ggtcccagtc gtgagcctct gtatgactgc cccctgacaa gtctgatgac
      961 tttatcaccg tcagcttctc cctggtacgc agcaataatc agcccgccaa tgtcagggtc
     1021 tccccatctg ttgcggacag agctcgccac gattctgtaa ataatatctt ctgtggttat
     1081 atttattttc a
//
LOCUS       DAJTSL010000332         1075 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTSL010000332 DAJTSL010000000
VERSION     DAJTSL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.336"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..842
                     /locus_tag="OO433_004218"
     CDS             <1..842
                     /locus_tag="OO433_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR6052459.1"
                     /translation="ALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNA
                     GSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGCLAHA
                     RRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYD
                     WIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVGRKN
                     YLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFS
                     SVK"
     gene            842..>1075
                     /locus_tag="OO433_004219"
     CDS             842..>1075
                     /locus_tag="OO433_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6052460.1"
                     /translation="MLTLRTSRGAYTVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCK
                     RVFQLTYSYEARKPGVKEQIVEMAHNGAGGRDTA"
BASE COUNT          277 a          253 c          322 g          223 t
ORIGIN      
        1 tagcgctgaa tgactatgtt ctggaggcag gaaaggtgca cgcagatgac actccggtga
       61 aagtactggc cccggggaac ggaaagacga aaacgggtcg tctgtgggta tacgtcaggg
      121 atgatcgtaa tgcgggttca tccctgccgg cagccgtctg gttcgcgtat tcggcagatc
      181 gcaaaggaga acatccgcag ctccacctgg caaagtatca gggcgtactg caggctgatg
      241 cctatgcagg ttataacgta ctgtacgaaa cgggccgggt gaaggaagcc gggtgcctgg
      301 cccacgcccg ccgaaaaatc catgacgagg atgtgcgccg tccgacagaa atgactcagg
      361 aagcgctcag acggatagca gagttatacg acatagaagc ggagatacgt ggcagtccgg
      421 cagaggaacg gcttgcagtc agaaaagcca gaagcgtcca gttgatgcag tcattgtacg
      481 actggataca gttgcagagg aaaacgctgt cgaaacatgc ggagatggcg aaggcgttcg
      541 actatatcct gaatcactgg aatgcgctga acgagttctg tcgtgacggc tgggtggaaa
      601 tagacaacaa catcggtgaa aacgcgttac gatcggtggc ggttggaaga aaaaattatc
      661 tctttttcgg ctcagacaag ggaggagaaa gtgcggcgat catctacagt ctgctggtca
      721 cctgcaaaca gaacgaagtg gagccggagg actggttgcg cgaagtgatc gagaagctca
      781 atgactggcc gtcgaaccaa gtgcatgaac tgctgccctg gaacttctcg tctgtaaaat
      841 aatccttacg ctacgtactt ctcggggcgc ttacaccgtt actgttcatt gtccgcgctg
      901 tcattccgat gaagtttatc gacatggccg tagttgttcc cggcatgagc gtttccgttg
      961 tcgctcatgt aaacgtgttt tccagctcac ttactcttat gaagccagaa aacccggcgt
     1021 taaggagcag atcgttgaaa tggcacataa cggcgcaggg ggccgtgata ccgcc
//
LOCUS       DAJTSL010000333         1062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTSL010000333 DAJTSL010000000
VERSION     DAJTSL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.337"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>1062)
                     /locus_tag="OO433_004220"
     CDS             complement(<1..>1062)
                     /locus_tag="OO433_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCR6052461.1"
                     /translation="DVTNCHFKGDVISKVSFLSSNLQHVTFECKEIENCNFTKATVDN
                     VIFKCRRLHNVIFLKTSGECVDFNQDILDTVDFSQSQLGHSNFRECQIRNSNFDNCYL
                     YASHFTRAEFLSAKEISFIKSNLTAVMFDHVRMSTGNFKDCITEQLELTIDYSDIFGN
                     EDLDGYINNIIKMIDTLPDNAMILKSVLAVKLVMQLKILNIVNKNFIENMKKTFSHCP
                     YIKDPIIRSYIHSGEDNKFDDFMRQHRFSKVDFDTQQMIHFINRFNMNKGLIDKNNNF
                     FIQLIDQALRSTDDMIKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRNDNI
                     FILFSSVDDGPVMVVSSQR"
BASE COUNT          360 a          183 c          157 g          362 t
ORIGIN      
        1 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
       61 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
      121 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
      181 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
      241 tatttttatc aatcagccct ttattcatat taaacctgtt aataaaatgg atcatctgtt
      301 gggtatcgaa atccaccttg ctgaatcgat gttgacgcat aaaatcatcg aacttgttat
      361 cttcaccaga atggatataa ctgcgtataa ttggatcttt tatataagga caatggctaa
      421 atgttttctt catattctca ataaagtttt tattaacaat attaagaatt tttaattgca
      481 taaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
      541 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
      601 aatcaatagt taattccaat tgttctgtaa tgcaatcttt aaaattccct gtcgacattc
      661 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
      721 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
      781 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
      841 tatcaagaat atcttggtta aaatcaacac attcgccact cgttttgaga aaaatcacat
      901 tgtgtaaacg tcgacattta aatatgacat tatcaactgt tgccttagta aaattgcaat
      961 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
     1021 tagaaataac gtcaccttta aaatgacaat ttgttacatc aa
//
LOCUS       DAJTSL010000334         1044 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTSL010000334 DAJTSL010000000
VERSION     DAJTSL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.338"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>1044
                     /locus_tag="OO433_004221"
     CDS             <1..>1044
                     /locus_tag="OO433_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6052462.1"
                     /translation="EHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRIT
                     TPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDIIRYRYDNPHS
                     DLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYR
                     AYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIRTTQGEL
                     TRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSE
                     DEGLVTHWHYDEADHLTHRTVNGETAEQWQYDKRGWLTDISHLSEGHRVTVHYRYDEK
                     GRLTGERQTVHHPQ"
BASE COUNT          261 a          306 c          316 g          161 t
ORIGIN      
        1 gaacacgcgg acggcagcgt cacgcagagt cagtttgacg ccgtgggcag gctcaggaca
       61 cagacggatg ccgcaggccg gacaacagaa tacagcccgg atgtggtgac gggcctcatc
      121 acgcgcatca ccacgccgga tggcagggca tcggcgtttt actataacca ccacagccag
      181 ttaacgtcag ccaccgggcc tgacgggctg gaaatacgcc gggaatatga tgaatggggc
      241 cgtctgattc aggaaactgc ccctgacggc gatatcatcc gctaccgtta tgataatcca
      301 cacagtgact taccctgcgc aacggaagat gccaccggta gccggaaaac catgacgtgg
      361 agccgttacg gtcagttgct gagcttcacc gactgttccg gttatgtaac ccgttatgac
      421 catgaccgct tcgggcagat gacggcggtg caccgcgagg aagggctgag tcagtaccgc
      481 gcatacgaca gccgtggaca gttaattgcc gtgaaagaca cgcagggcca tgaaacgcgg
      541 tatgaataca acgccgccgg tgacctgacc gccgtcattg ccccggacgg cagcagaaac
      601 gggacacagt acgatgcgtg gggaaaggcc atccgtacca cgcagggcga gctgacgcgc
      661 agtatggaat acgatgctgc aggacgggtc atccgcctga ccagtgaaaa cggcagccac
      721 accaccttcc gttacgatgt actcgaccgg ctgatacagg aaaccggctt tgacggccgc
      781 acacagcgtt atcaccacga cctgaccggc aaacttatcc gcagcgagga tgaggggctg
      841 gtcacccact ggcactatga cgaagcagac cacctcacgc accgcaccgt gaatggcgaa
      901 accgcagagc agtggcagta tgacaaacgt ggctggctga cagacatcag ccatctcagc
      961 gaagggcacc gggtgacggt gcattacagg tatgatgaga aaggccggct gaccggtgag
     1021 cgccagacgg tgcatcaccc gcag
//
LOCUS       DAJTSL010000335         1010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTSL010000335 DAJTSL010000000
VERSION     DAJTSL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.339"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO433_004222"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO433_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6052463.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO433_004223"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO433_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6052464.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGTPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..>1010)
                     /locus_tag="OO433_004224"
     CDS             complement(941..>1010)
                     /locus_tag="OO433_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6052465.1"
                     /translation="PFGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          301 a          206 c          231 g          272 t
ORIGIN      
        1 gctaaagttc tgggttaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataatcat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaac ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaattg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc
//
LOCUS       DAJTSL010000336          927 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTSL010000336 DAJTSL010000000
VERSION     DAJTSL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.340"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(237..332)
                     /locus_tag="OO433_004225"
                     /pseudo
     CDS             complement(237..332)
                     /locus_tag="OO433_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(332..415)
                     /locus_tag="OO433_004226"
                     /pseudo
     CDS             complement(332..415)
                     /locus_tag="OO433_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            complement(445..774)
                     /locus_tag="OO433_004227"
                     /pseudo
     CDS             complement(445..774)
                     /locus_tag="OO433_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          245 a          217 c          228 g          237 t
ORIGIN      
        1 gcggggctga atgtggaggg gctacgcgcg ggatatcagt ctgctgaggt atatgccggc
       61 ctgacacagg ctaagtgtgc ccgggaatcg gtgcaagtat aaaaaacagc ctgttgcggt
      121 ataagaatcg accatgcaga gagagaaaac accggagtgg agggaaaagc agaaaagctc
      181 acgtggcata aggcgtggac agcggtatcg tctggttttt caatttccta tacgtgtacc
      241 attctggcgg cagcaggttt actggccagc accagaaaat catataaccg cgccaaatca
      301 gaaagcgcct tactggtcca cttctgttcc atcagcgtgg caccggtaac ggattgtcag
      361 tactgaggct gtccgcccag gcctgtacag cctgatggtc gataatctgt ccggatggac
      421 cagcgggagc atttgaacag aaacttacgg ttttgctctt tcggtaggct cagtaccgta
      481 aggttctcca tcaacaaaat aagtaccatt aggtaatacg tgtttatagg gcagtaatga
      541 tatatgggct aatacgtcat catcaataac ctcaccattt tcacgtaacc cttcaacaat
      601 attgcttatt cttgatgtat tccagtacag gattgcattc gataccagac tcagggcgct
      661 ggccttattc atgatttctt cgtaatcacc ggtcgtgaat tcaccctgat ttgcaaaaaa
      721 gatccatcgg ggtaatttat gtcgatattc gcctttattg agctgaagct cgagtaggca
      781 gcctgacggc tgcggcttgt catggtctgc aattaccgtt ataaaaacag gcaatgtcat
      841 tgtctttcag gtggttatat ggcccgttca gctaaacccc gtaaacgtaa acccgcctct
      901 caaagaagca aacttccccg ctatgtc
//
LOCUS       DAJTSL010000337          875 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTSL010000337 DAJTSL010000000
VERSION     DAJTSL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.341"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..603
                     /gene="ospC4"
                     /locus_tag="OO433_004228"
     CDS             <1..603
                     /gene="ospC4"
                     /locus_tag="OO433_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6052466.1"
                     /translation="IGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYH
                     IPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIA
                     LYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNK
                     KFQKANSGDTMLDNAMKSKNPKMIDFLLKNGAILGKRFEI"
BASE COUNT          308 a          136 c          172 g          259 t
ORIGIN      
        1 attgggttgc atcttattga ttttataaga aagagtaaag atcaaggatt cagagagttt
       61 tgttataaca aaaatattga tcctgttagt ctggatagaa ttataaactt tgtgtttcag
      121 cttgagtatc atataccaag aatgctaagt acagataact tcaaaaaaat aaaactcaga
      181 gacatatctt tggaggatgc gattaaagca tctaattatg aagagattaa caataaagtg
      241 actgataaaa aaatggctca tcaggccctt gcttattctt taggcaataa aaaagctgat
      301 atagcacttt atttactatc taaatttaat tttacaaaac aagatgtcgc agagatggag
      361 aaaatgaaca acaatagata ttgtaatctg tatgatgttg aatacttact tagtaaagat
      421 ggtgccaact ataaagtgct agaatatttt atcaataatg gattggttga tgtaaacaaa
      481 aaatttcaaa aagcaaatag tggggacact atgcttgaca atgcaatgaa aagcaaaaat
      541 ccaaaaatga ttgatttttt attaaaaaat ggagcaatat taggcaaacg atttgagata
      601 tgacctaaaa gctcggccaa ataataatgt cgtcgattat cgaagtcatc atgagtgatt
      661 ctgtccccac agtgcagcat cagcatgaca gtgaattgtc tggcgtggtc catttaatga
      721 gatggagatg gtccctcttt cctaaatagc tgtgccgaat accgctacac ttttgccagc
      781 ccatgtttgc ctccggggaa tgggctgacg gttccttcaa aatccattgc acgcagttat
      841 gtcggagcgg gaacttgact cctgtcttta cggga
//
LOCUS       DAJTSL010000338          869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTSL010000338 DAJTSL010000000
VERSION     DAJTSL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.342"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..314
                     /locus_tag="OO433_004229"
     CDS             <1..314
                     /locus_tag="OO433_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6052467.1"
                     /translation="LAEVGGITGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQ
                     TKRLICLDAAKLTVPIQAYIYRMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARR
                     N"
     gene            complement(432..869)
                     /gene="tnpA"
                     /locus_tag="OO433_004230"
                     /pseudo
     CDS             complement(432..869)
                     /gene="tnpA"
                     /locus_tag="OO433_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          218 a          234 c          178 g          239 t
ORIGIN      
        1 atctggcaga ggttggaggt attaccgggc gcacgatatt tatctcttcg atgagcgagt
       61 tattaaagaa tatggcgctc aacggatacg gcattgcctg gttaccgatc tggtcgatcg
      121 tcgatgaact acagaccaaa cgtctgatat gccttgatgc tgctaaattg acagtcccca
      181 ttcaggctta tatttaccga atgaacaccc gactgaatag aaccgccgag aacctctggc
      241 gtattttaca ggaacatatg ccagacgatt taatccagca gatatcaatg gaagagccag
      301 cacgccgcaa ttgacgacgt gcaataaata tcctccggca tagccggagg tttttcaaat
      361 gcgcctataa ggctctctta ccagccgcgc cctaacaggc gcacacgatc tgacatttgc
      421 atccaacttc gttacttacg gtccgtaaac gggctgcccg gacagggaat cgataactgc
      481 tctcccattt tatcctcttc aagctggtgc tttatgtaat cctgtatctt cgccgtgttc
      541 ttacccactg tatcgacgta gtcccccctg caccagaact ccctgttcct gtatttgaat
      601 ttcaaatcac caaactgctc gtaaagcatc agactgcttt tccctttcag atatcccata
      661 aagccggata cgctcatttt gggcgggatc tccacaagca tatggatatg atctgcacag
      721 cattcagctt ccagaatccg tacgcttttc cactcacaca gctttctcaa aatactgcct
      781 gttgctctac gcttctctct gtagaacacc tgtcttcggt attttggcgc aaaaaactat
      841 gtgatattta cagttccatc gggtgtgcg
//
LOCUS       DAJTSL010000339          855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTSL010000339 DAJTSL010000000
VERSION     DAJTSL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.343"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..831
                     /locus_tag="OO433_004231"
     CDS             <1..831
                     /locus_tag="OO433_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6052468.1"
                     /translation="YIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQCIGV
                     EILLRWNNPRQGRISPDVFIPIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHVGIN
                     VAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVDYRIARELHRKNVKLAI
                     DDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIELVAE
                     GVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPIMPLR
                     "
BASE COUNT          199 a          218 c          217 g          221 t
ORIGIN      
        1 tacatcgcct ggctggcgac cgcttaccgg atgagctttt cccgcgaaat caatctgggc
       61 ctggcgcaac atgagttcga attgttctgt cagcctttgc ttaatgcgcg cagccagcaa
      121 tgtattggtg tagagatttt gctgcgctgg aacaatccgc gtcagggccg gatttcaccg
      181 gatgtgttta ttcctatcgc ggaagaacat catttaattg tgccactgac ccgctatgtg
      241 atggcagaaa ccattcgtca gcgccatgtt ttcccgatga gtagtcagtt tcatgttggc
      301 attaacgtcg cacccagcca ttttcgccgt ggtgtgctga taaaagatct caatcagtac
      361 tggtttagcg ctcacccgat tcagcaactg atcctcgaaa tcaccgaacg cgatgcctta
      421 ctggatgttg attatcggat tgcccgcgag ctacatcgta aaaacgtcaa actggcgatt
      481 gatgacttcg gcaccggcaa cagttcattt tcctggcttg aaacattacg tcctgacgtg
      541 ctgaaaattg ataagtcatt taccgcagct ataggttctg acgcggttaa ctcgacggtg
      601 accgatatca tcatcgcgct ggggcaaaga ctgaatattg aactggtggc ggagggcgtg
      661 gaaacgcaag aacaggcgaa gtatttgcgc cgtcatggcg tgcatatttt gcaagggtat
      721 ttgtacgcac agccgatgcc gctacgcgat tttcccaaat ggctggcggg cagccaaccg
      781 ccgcccgccc ggcataatgg acatatcacg cccattatgc cgttacgtta acgctgatat
      841 gactttccgt gtcaa
//
LOCUS       DAJTSL010000340          805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTSL010000340 DAJTSL010000000
VERSION     DAJTSL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.344"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..619)
                     /locus_tag="OO433_004232"
     CDS             complement(<1..619)
                     /locus_tag="OO433_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6052469.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERHTME
                     ETEAKPWLGESVDRRTLKDVVELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGGIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGA"
BASE COUNT          193 a          196 c          178 g          238 t
ORIGIN      
        1 gtgcgcctgt gcttaagcat atcttaacta ccagtgccag aattgggtcc tgacgtttgc
       61 aatcagccag caattcaaca atctgctcat gggtaagcca tgccatctct ttttctgcga
      121 tggtgaattt tcgcatgttc tccagtgggt tcggatacga ccattcgccc agacgggata
      181 gttcgctaaa aacactactt agatagcttt gctccaggtt aatggtgacc gggcttgctc
      241 ctttcttcca tttctcgctg aagtagatcc cgcctgtcag gcgtttatct cgatagtggg
      301 caaacatttt agaggtgaga tcggttgcaa gaggattgcc cagagcgtca accatcaaca
      361 gcaatttgtc atagacatgc tgcccagctg tcagagattt accatgtagt ttgaaccata
      421 gctcaaccac gtctttcagt gttcgacgat ccactgattc acccagccag ggctttgctt
      481 cggtttcttc catcgtgtgg cgctcaaaag ccagtgcttc gcctttggtg gcgaattgtt
      541 tacgcacacg acgcccactt cgtccggcgg ggtaacattc gcaaagccat ttcccagtgg
      601 tgagttttcg tactgccata aaaaatgccc tccagtagag agtattttta ctgtatgtat
      661 aaccagtgtc aatgtatgaa atcctacgac catacatctc actgaagcca taatgaagtt
      721 ggctattctt tttgctatgt gagcatgtaa cttttgcggt taacctgcgg ctcattttta
      781 ttttagacgc agatataaaa gcaaa
//
LOCUS       DAJTSL010000341          804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTSL010000341 DAJTSL010000000
VERSION     DAJTSL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.345"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>804
                     /locus_tag="OO433_004233"
     CDS             <1..>804
                     /locus_tag="OO433_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6052470.1"
                     /translation="GLDIAELQSQFAGFGASAKESMGIQGSEELFRNMIGYSRLMGRS
                     EEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKSEQELFDAMKKG
                     ALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSENGL
                     QDFTRSLTTLLNSLGGSGKSLGESLGSLMTSMSSGVDSLTDISYRVRAFFDEVTLAYR
                     GLTDEQKKVVDALGSGLINSLKVLAGVIAVNKVVGVTRSIYGLMTAITR"
BASE COUNT          237 a          125 c          209 g          233 t
ORIGIN      
        1 cgggtttaga tatcgctgaa ctgcaatcac agtttgctgg ttttggtgct tcagctaaag
       61 agtctatggg tattcaaggt tctgaagagc tttttagaaa tatgattggc tattctcgtt
      121 taatgggtcg aagtgaggaa gagattaagc gagcactgac agcattatca cagatggctg
      181 gtaagggtca gattatggct gaagagctta aggggcaact agctgaagct gttcctggta
      241 tggttcaggt attcgctaaa gcaaccggaa agagtgaaca ggaattattt gatgccatga
      301 agaagggggc tttaaaatct gccgatactc ttcagaaggt cactcaggaa ctgaataaac
      361 aaatcacagc taaaggtggc tggaaagcta tttcagaaag cacacaagca caactgggga
      421 acctgaaaaa ctcttggaat accacgcttg atagtatctt cagaggttct gaaaatggtc
      481 tgcaagattt tacacgaagc ctaacaacgc ttttaaacag tttaggtgga agtggtaaga
      541 gtcttggtga atctcttggt agcttgatga cttctatgtc aagtggtgtg gatagcctga
      601 ccgatatcag ttacagagtg agagcattct ttgatgaggt gacattagct tatcgtggtt
      661 taaccgatga acaaaagaaa gttgttgatg ctttaggttc tggcttgatt aatagtttaa
      721 aagtcttggc tggtgttatt gctgtgaata aagttgttgg tgtaaccaga agtatttacg
      781 ggcttatgac tgctatcaca agat
//
LOCUS       DAJTSL010000342          788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTSL010000342 DAJTSL010000000
VERSION     DAJTSL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.346"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>788
                     /locus_tag="OO433_004234"
     CDS             <1..>788
                     /locus_tag="OO433_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6052471.1"
                     /translation="EKIEAQRESLATLKGEFIDGKYMRVHRTIGSTRMDIKIAHARIE
                     NKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILKNHAHDSIGCCCSDKVHREIVARF
                     ELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNLMPWPREEVINTTVRLRASQFNLR
                     DDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPFMEFDIQINQIVPSMGYRTLYIEA
                     NQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLVDKDSGVRYD"
BASE COUNT          212 a          182 c          200 g          194 t
ORIGIN      
        1 ttgagaagat cgaagcgcag cgtgaaagtc tggccaccct gaaaggtgaa tttatcgatg
       61 gcaaatatat gcgcgtacat cgcaccatcg gttccacgcg catggatatc aaaattgccc
      121 acgcacgtat tgaaaataag attgttaatc tgctggaacc gctggcaacg ctggcctgga
      181 cgttgggttt tgaataccac cacggcttgc tggagaaaat gtggaaagag atcttaaaaa
      241 atcatgccca cgacagtatc ggttgttgtt gcagtgacaa agttcatcgc gaaattgtcg
      301 cccgcttcga actggctgaa gacatggcgg ataatctgct gcgtttctac atgcgcaaaa
      361 ttgccgataa catgccacag agtgatgccg acaaactcgt gctgtttaac ctgatgccct
      421 ggccgcggga agaagttatt aacaccactg tgcggctgcg cgccagccag tttaatctac
      481 gagacgatcg cggtcagcct gtaccgtatt ttattcgcca tgcccgtgag atcgatccag
      541 gcctaatcga tcggcaaata gttcattacg gtaattacga tccctttatg gagtttgata
      601 tacagatcaa ccagattgtc ccttctatgg gctatcgcac gctttatatc gaagcgaatc
      661 agcctggcaa cgtgattgcg gcaaaaagtg acgctgaagg gatactggaa aatgctttct
      721 ggcaaattgc gctcaatgag gatggttctc tgcaactggt agataaagac agcggtgtgc
      781 gctatgac
//
LOCUS       DAJTSL010000343          776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTSL010000343 DAJTSL010000000
VERSION     DAJTSL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.347"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>776
                     /locus_tag="OO433_004235"
     rRNA            <1..>776
                     /locus_tag="OO433_004235"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          192 a          182 c          250 g          152 t
ORIGIN      
        1 actccgtgcc agcagccgcg gtaatacgga gggtgcaagc gttaatcgga attactgggc
       61 gtaaagcgca cgcaggcggt ttgttaagtc agatgtgaaa tccccgggct caacctggga
      121 actgcatctg atactggcaa gcttgagtct cgtagagggg ggtagaattc caggtgtagc
      181 ggtgaaatgc gtagagatct ggaggaatac cggtggcgaa ggcggccccc tggacgaaga
      241 ctgacgctca ggtgcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg
      301 ccgtaaacga tgtcgacttg gaggttgtgc ccttgaggcg tggcttccgg agctaacgcg
      361 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
      421 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      481 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      541 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      601 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      661 aactggagga aggtggggac gacgtcaagt catcatggcc cttacgacca gggctacaca
      721 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctca
//
LOCUS       DAJTSL010000344          764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTSL010000344 DAJTSL010000000
VERSION     DAJTSL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.348"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..648
                     /gene="ospC2"
                     /locus_tag="OO433_004236"
     CDS             <1..648
                     /gene="ospC2"
                     /locus_tag="OO433_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6052472.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFINNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLRNGAVSGKRFGR"
BASE COUNT          279 a           99 c          151 g          235 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat atcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattaa taatggattg gttgatgtaa acaaaagatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aagattcaaa aacaattgat
      601 tttttattaa gaaatggagc ggtatcaggc aaacgatttg ggaggtgacc taaaatatat
      661 ttaggttgtg ccctgaatct gacgttgtca tttttgttat attgatcgat ataggggatg
      721 gtgcagaccg ttcccctgca acaacacatt gcatgtggtg aacg
//
LOCUS       DAJTSL010000345          746 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTSL010000345 DAJTSL010000000
VERSION     DAJTSL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.349"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..97)
                     /locus_tag="OO433_004237"
     CDS             complement(<1..97)
                     /locus_tag="OO433_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6052473.1"
                     /translation="MKACLLLFFYFSLIDQLHGADVKIKENESVMG"
     gene            complement(109..>746)
                     /locus_tag="OO433_004238"
     CDS             complement(109..>746)
                     /locus_tag="OO433_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HCR6052474.1"
                     /translation="ESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVINV
                     DPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLI
                     KFDGNYKDGMTVWLFSCNTSKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKM
                     DTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          209 a          169 c          130 g          238 t
ORIGIN      
        1 atcccatcac actttcgttt tcttttattt tcacatcagc accatgcaat tgatcaataa
       61 gagaaaaata aaaaaatagt aacaagcatg ctttcataca tctacaacct atcttatatc
      121 actgggtttt tctggcgtac cttgcatatt agaaatgggg tgcccagatg gcccatatgt
      181 tatccaatta ccaaggtctt ttgttgttat agccattaga tccttatttg aattaaggtt
      241 cgttggtgct gttagcactg tatccatttt taacttacca ttagttcctc gcccccacca
      301 cgtccatagc gtgtcaggtc ctattacatt tgtatgtaac tctttagcta attggctagc
      361 aaatgaattt tgtcctttac ttgtattaca agaaaataac caaactgtca tcccatcttt
      421 ataattccca tcaaatttaa ttagatatgc tagatcttta gctgtcatga tactgcgcgt
      481 tgcggattca atagatgtgg gggtaccatg ccccccgatt gtgaaaacgc ctgggatatt
      541 aatctcatca gctacagaat ggataagatc actttcgggg tataaattta tatcaaccaa
      601 accttgcgga tctacattta tgaccggatt caacggatac tgataaaaat tccatccccc
      661 cttcagccca atcggatcct gagtgatata ccgcccctgc agcgggtcat aatagcggtg
      721 gcggttgtaa tacaggccgg actcct
//
LOCUS       DAJTSL010000346          704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTSL010000346 DAJTSL010000000
VERSION     DAJTSL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.350"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>704)
                     /locus_tag="OO433_004239"
     CDS             complement(<1..>704)
                     /locus_tag="OO433_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6052475.1"
                     /translation="AQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITG
                     AAGARGIYAMTNSQIDLTSDLVIDMSTPDKMAIATQHDDGYAASRINASGRMLINGSI
                     FSKGGLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTV
                     DFASHGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVL
                     AIRNQGSEATTGNEVL"
BASE COUNT          162 a          195 c          163 g          184 t
ORIGIN      
        1 tcagaacttc atttcccgtt gtggcctcgc tgccctggtt gcggatagcc aatacgtgat
       61 taccagcact actcccgctg atattcaata aatccccttt attattaacg ccattaccct
      121 cgccaacaac atcagcacgc ataataaagg tactgttacc gctcaggttc tctacgttta
      181 atgtggcaaa tgtgccggca gttgacccgt ggctggcaaa atcgacagtt gaatggctca
      241 gcgccagcgt gtcgagatta gaattacttg ttacgttcca gacgctgtta ttcattgcaa
      301 cgtccagttt tccgccattg acattatcgc tgagggagga acctgtccaa accgacccag
      361 ggtgcatatc cagattgatt agcccacctt tggaaaaaat gctaccgttg ataagcatac
      421 gacccgaggc gttgatgcgg ctggcggcat aaccatcgtc atgttgcgtt gcaatggcca
      481 tcttgtcggg tgtactcata tcaatgacca gatcgctcgt gaggtcgatc tggctgttgg
      541 tcatggcata aattcccctg gctcctgccg cgccggtgat agccaaactg tctccggtta
      601 ttctaccgcc gctgagcgcc cacaaaccca gcgccagact gccattacga tcaactgtaa
      661 tatcggtatt ttgcatgttg acaacagccg ttgccgtctg ggcc
//
LOCUS       DAJTSL010000347          686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTSL010000347 DAJTSL010000000
VERSION     DAJTSL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.351"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>686
                     /locus_tag="OO433_004240"
     CDS             <1..>686
                     /locus_tag="OO433_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6052476.1"
                     /translation="ENYLTHWTEWKNNRIQEEQRDIAFQRLVSCLQNQETNLDLSELG
                     LTTLPEIPPGIKSINISKNNLSLISPLPASLTQLNVSYNRLIELPALPQGLKLLNASH
                     NQLITLPTLPISLKELHVSNNQLCSLPVLPELLETLDVSCNGLAVLPPLPFSLQEISA
                     IGNLLSELPPLPHNIHSIWAIDNMLTDIPYLPENLRNGYFDINQISHIPESILNLRNE
                     CSIDISDNPL"
BASE COUNT          229 a          158 c          103 g          196 t
ORIGIN      
        1 atgaaaatta tctaacccat tggactgaat ggaaaaataa ccgcatacaa gaagaacaac
       61 gagacatcgc ttttcagcga ctagtatcat gtctacaaaa ccaagagacg aacctagact
      121 tgtctgaatt aggcctgaca acattacctg aaatcccccc gggaattaaa tcaattaata
      181 taagtaaaaa taatttaagc ttaatctccc cattgcctgc gtcccttaca cagcttaatg
      241 tcagctataa cagacttatt gaactgcctg ctttgcctca aggacttaaa ttattgaatg
      301 cgtcccacaa tcaactaatc acactaccca cactccccat atctttgaag gagcttcatg
      361 tctcaaataa tcaattatgt tctcttcctg ttttaccaga actactggaa acattagatg
      421 tatcatgtaa tgggctggca gttttaccac ctttaccatt ttctttacaa gagattagcg
      481 caatagggaa tcttcttagt gaactccccc ctctacctca caacattcac tccatatggg
      541 caatcgacaa tatgttaacc gatattccat acctgccgga aaatttaagg aacggttatt
      601 ttgacataaa tcagataagt catatcccgg aaagcattct taatctgagg aatgaatgtt
      661 caatagatat tagtgataac ccattg
//
LOCUS       DAJTSL010000348          675 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTSL010000348 DAJTSL010000000
VERSION     DAJTSL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.352"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            193..462
                     /locus_tag="OO433_004241"
     CDS             193..462
                     /locus_tag="OO433_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052477.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..675
                     /locus_tag="OO433_004242"
                     /pseudo
     CDS             459..675
                     /locus_tag="OO433_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          170 a          154 c          197 g          154 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaa
//
LOCUS       DAJTSL010000349          631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTSL010000349 DAJTSL010000000
VERSION     DAJTSL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.353"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            106..>631
                     /locus_tag="OO433_004243"
     CDS             106..>631
                     /locus_tag="OO433_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCR6052478.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEH"
BASE COUNT          162 a          170 c          167 g          132 t
ORIGIN      
        1 cctaaatagc tgcgcggaat agtagatccc tttgagggaa cttagcccgg attgtgcgat
       61 ctgatcaatc gccaaatcaa aacaaatcac caaccggact gagcaatgcc gatcatagca
      121 ccaatttccc gtgacgaacg acgcctgatg cagaaagcca tccataaaac acacgataaa
      181 aattatgccc gcagactgac tgccatgctg atgctgcacc ggggcgaccg tgtcagcgac
      241 gttgccagaa cgctctgctg cgcccgttcc tctgttggac gctggattaa ctggttcacg
      301 cagtcgggtg ttgagggact gaaatcatta cctgccgggc gtgcccgtcg ctggccgttt
      361 gagcatatct gcacactgtt acgtgagctg gtaaaacatt ctcccggcga ctttggctac
      421 cagcgttcac gctggagtac agaactgctg gcaataaaaa tcaatgagat aaccggttgc
      481 cagttaaatg ccggaaccgt tcgccgctgg ttgccgtctg cggggattgt gtggcgaagg
      541 gctgcgccaa ctctgcgtat ccgtgacccg cataaagatg aaaagatggc agcaatccat
      601 aaagcactgg acgaatgcag cgcagagcat c
//
LOCUS       DAJTSL010000350          615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTSL010000350 DAJTSL010000000
VERSION     DAJTSL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.354"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>615
                     /locus_tag="OO433_004244"
     CDS             <1..>615
                     /locus_tag="OO433_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6052479.1"
                     /translation="APQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQG
                     QLDVKVEEEDGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGE
                     FSWGWLNNVSLHGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDTQLHNQDKETG
                     YSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTN"
BASE COUNT          175 a          137 c          127 g          176 t
ORIGIN      
        1 gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc acagaacaac
       61 cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa tttattcaat
      121 acattacagg ggcaactgga cgtcaaggtt gaagaagagg acgggcaagt cacgcaatgg
      181 caagttgcat ctaatagtat tccttatctg acgcgtaaag ggcagattcg ctacaccact
      241 gctatgggta aaccgaccag cgttggtggt gattccttac aacaaccctt cttctggact
      301 ggtgaattct catggggttg gctgaacaat gtatccctgc atggtggttc agttttaaca
      361 aaccgtgatt atcaatctct ggctgccggc gttggcttta accttaactc attgggttca
      421 ttatcttttg atgtcacacg atctgatact cagttgcata atcaggataa agaaacgggt
      481 tatagctacc gcgctaacta ttcaaaacgt tttgaatcta ccggtagcca gctcactttc
      541 gctggttacc gtttctctga taaaaacttt gtgacaatga atgaatatat caatgacact
      601 aaccattaca cgaat
//
LOCUS       DAJTSL010000351          601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTSL010000351 DAJTSL010000000
VERSION     DAJTSL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.355"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..125
                     /locus_tag="OO433_004245"
     CDS             <1..125
                     /locus_tag="OO433_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6052480.1"
                     /translation="SPSQWAAIGVLGSLLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            130..474
                     /locus_tag="OO433_004246"
     CDS             130..474
                     /locus_tag="OO433_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR6052481.1"
                     /translation="MKKKYELVVKGINNYPNKITVTVALEIGGYPSLLLPDVAISLDR
                     TEDATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            525..>601
                     /locus_tag="OO433_004247"
     CDS             525..>601
                     /locus_tag="OO433_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6052482.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPE"
BASE COUNT          160 a          122 c          171 g          148 t
ORIGIN      
        1 tttccccgtc tcagtgggcg gcaataggcg tgctggggag tctgttgttt gggctgctga
       61 catatctgac taacctgtat ttcaaaatca gagaggaccg tcgtaaggcg gcgcggggag
      121 agtaaagcga tgaagaaaaa atacgaactg gttgttaaag ggataaataa ttacccgaat
      181 aagattactg ttactgtggc actggaaatt ggtgggtatc cgtcactgtt gttgccagat
      241 gtggcgatta gtcttgaccg tactgaagat gccacgctgg agttttacga agctgaggcg
      301 aaaaagcagg cgaagcagtt tttcatggat gttgctgccg ggttatgtga aggggatggt
      361 ccgttgccgg aaaagcgccc cgtaatttta gaggcgcagg atgtgttgat aacctacaga
      421 ggaaaactac cgggaataat tactggttct ctgaagactc caccgctggc ctgaagactt
      481 aacatatcca gggatttgaa atcgataaac cctgataaat atccatgaac accaaaatca
      541 aatacggcct gtcggctgcc gttctggcgc tgattgccgc aggtgcgcct gcgcctgaaa
      601 t
//
LOCUS       DAJTSL010000352          597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTSL010000352 DAJTSL010000000
VERSION     DAJTSL010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.356"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            107..576
                     /locus_tag="OO433_004248"
                     /pseudo
     CDS             107..576
                     /locus_tag="OO433_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          147 a          146 c          144 g          160 t
ORIGIN      
        1 tttaacgtaa ggtgagagga tttttaagtg tatccataga ttaaaaatga caatgcctcc
       61 aattagttga attaggggtt ataatacccg cttttaaggt tctttcatgg tcacagttaa
      121 ctttcattgc ccccgttgcc agtccgttca ggtttaccgt cacgggcaga acccaaaagg
      181 acatgatcga ttccgttgcc gtgactgtca ccgggtgttt cagctcactt actgttacga
      241 ggcccgtaaa ccgggagtta aaaaccaaat cactgaaatg gcttcaacgg tgccggagtt
      301 cgcgatactg cccgtacgct gaaaatcggc atcaataccg tcatacggac attaaaaagc
      361 tcccgccccg gcggataaca tctgcgccag tcgctcacgc tgatgttgcc cttatctgtg
      421 aacttgatga gcagtggcgc ttcgtcggca gcaaggctgc aacaacactg gctctggtat
      481 gcgtacaaca ctaaaactgg cggtgtgttg gcttacactt tcgggccacg gaatgatgag
      541 acctatcggg aactgttagc cctgtttact ccgttctgta tatattaggc cgttggt
//
LOCUS       DAJTSL010000353          592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTSL010000353 DAJTSL010000000
VERSION     DAJTSL010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.357"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            312..592
                     /locus_tag="OO433_004249"
                     /pseudo
     CDS             312..592
                     /locus_tag="OO433_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          156 a          148 c          150 g          138 t
ORIGIN      
        1 acgggaatat ctgcggcagc gggcttgtaa tgggttaagt gataacagat gtctggaaat
       61 ataggggcaa atccacatcc ccgccagccc ggctaagatg gaggcacggc acagcccgca
      121 cacgacgctg aggctacaga gctgaaaacc cacaaggcca tgccccgcat ggccgggtaa
      181 agcggagccg gcggcgactt cctttgtgct ctgtccggga ccggcaggaa aaaggcgggg
      241 ataatgagat ggtcaccccc accctaacag cagtacgctg ttaaggtgaa tcccttaacg
      301 gagagaccat catacagaac atcacactta ttggtatcga tctcggaaag cattccttcc
      361 acattcattg tcaagacaag tctggtaagg ctcttctgcg taaaaaattt acccagtact
      421 aaactgatgc agtttctggc aacatgtccg ccctcagttg ttgtaatgga agcctgtgcg
      481 ggtgcacact ttttggctcg ccgtatctct gaattttgga catgaaacta agctgatttc
      541 tccacagttc gttcgcccgt ttgtcaaaag taataagaat gattttgttg at
//
LOCUS       DAJTSL010000354          583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTSL010000354 DAJTSL010000000
VERSION     DAJTSL010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.358"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..375)
                     /locus_tag="OO433_004250"
     CDS             complement(<1..375)
                     /locus_tag="OO433_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6052483.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKK"
     gene            complement(401..>583)
                     /locus_tag="OO433_004251"
     CDS             complement(401..>583)
                     /locus_tag="OO433_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6052484.1"
                     /translation="TPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPVNVV
                     CRGNPAVVIRERVETE"
BASE COUNT          134 a          160 c          144 g          145 t
ORIGIN      
        1 tttcttttcc agaccgagga agcggatcgc ctccagcagg cgcagcgtac ccatcgcgtc
       61 gacgtcagcg gtatattctg gtgactcaaa agagaccgca acgtggctca ttgcgcccag
      121 gttgtacact tcatccggct gtacttcacg caaaatgcgc gtcaggttag aggtatcact
      181 caggtcgcca taatgcagat ggaatttcgg gttgcaggtg tgcggatcct gataaatgtg
      241 atccacgcgc tcggtgttga atgacgatgc gcgacgctta ataccatgca cctcgtaacc
      301 tttttccagc agaaactctg ccaggtaaga accgtcttgt ccggttacac cggtgatgag
      361 agcgactttt gacatgtctt attcctctgt atttttgaat ttattcagtt tcaacgcgtt
      421 cgcgtatcac cactgcggga ttcccccggc aaaccacatt taccggaagc gatttaaaaa
      481 cactgcttcg tgcacccacg acggtgccgt cgccgattgt gacgcctggg gcaacaaaga
      541 catcggttgc cagccagcat ttctcgccaa tcacaatagg cgt
//
LOCUS       DAJTSL010000355          573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTSL010000355 DAJTSL010000000
VERSION     DAJTSL010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.359"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..565)
                     /gene="ospC4"
                     /locus_tag="OO433_004252"
                     /pseudo
     CDS             complement(1..565)
                     /gene="ospC4"
                     /locus_tag="OO433_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
BASE COUNT          177 a           81 c          107 g          208 t
ORIGIN      
        1 gggagttttt cttgatgatt ggaaatttca accccaaaaa aaacaaaatc gtggttagag
       61 atttctttaa tatcctcaga gtatgtattc ttatgtgttt gtatattttc agactgaatt
      121 ttatcaaagg atttaatatt aagaatatta tttgacagat tagagttact ttgatgtctg
      181 aagttaaaat tagaattaat aataatagaa agtaagtgac gttcgtctga agaaagagga
      241 tgaacttttt gtattatctc agagatatag tgcctggaca ccttatcgag atgctcaagt
      301 gaccgaaatg atgaccgtgc tccagatggc gcttcaatag aaagatttag tgatttgaaa
      361 ctggtccctt gagaaaacat tttgctataa ctttgcgctg ctatagactt ccttaaaaag
      421 tttttcacag tattagcaca gtgatctaaa tgtttttcac ttatgccaga agaagatgag
      481 tttgttacgc gtaatatatt ttttgtaatg cttttgttgc tttattcgga ttcgtttttc
      541 catcaacaag tggatttgcc cctatatttc cag
//
LOCUS       DAJTSL010000356          553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTSL010000356 DAJTSL010000000
VERSION     DAJTSL010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.360"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>553
                     /locus_tag="OO433_004253"
     CDS             <1..>553
                     /locus_tag="OO433_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6052485.1"
                     /translation="GLVVVFSWVFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHT
                     MGPGLAYLAAWTYWVVHIPYLAQKPQAILIALGWAMKSDGSLIKEYSVVALQGLTLVL
                     FIFFMWVASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETF
                     IPHIDFTYITTISMLVFAVGGAEK"
BASE COUNT          116 a          120 c          153 g          164 t
ORIGIN      
        1 ggggctggtg gttgtttttt catgggtgtt tatctttgcg ctctatttca caccttatgc
       61 gctaattgtt ggtcagttag gctcgacctt caaagatggg aagggcgggg tcagtacctg
      121 gattaaacac acgatgggac ccggactggc ttatctcgcc gcgtggacct actgggtggt
      181 gcatattccc tatctggcac aaaaacccca ggcaattctg attgcgctcg gttgggcgat
      241 gaaaagcgac ggttcgctaa tcaaagaata ttcagtcgta gcgttacagg ggttaacgct
      301 ggtgctgttt atcttcttta tgtgggttgc ttcacgcggt atgaaatcgc tgaaaatcgt
      361 cggttctgtg gcagggattg cgatgtttgt tatgtcgctc ctgtatgtgg cgatggcggt
      421 aaccgcgcct gcgattactg aagtgcatat tgcgaccaca aacattacct gggaaacgtt
      481 cattcctcat atcgacttta cctacattac cactatttca atgctggttt tcgcggttgg
      541 cggagcagag aag
//
LOCUS       DAJTSL010000357          530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTSL010000357 DAJTSL010000000
VERSION     DAJTSL010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.361"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>530
                     /locus_tag="OO433_004254"
     CDS             <1..>530
                     /locus_tag="OO433_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HCR6052486.1"
                     /translation="YYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELA
                     ENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVW
                     VADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGL
                     IHHSDRGSQYCAYDYR"
BASE COUNT          145 a          139 c          145 g          101 t
ORIGIN      
        1 gttattacaa ctgggtacag catgaaccct cagacagaaa acaaagtgat gagcggctaa
       61 aactggagat taaggtggca catatccgca ctcgcgaaac atatggaacc cggcggctcc
      121 agacggagct ggcagagaat ggcatcatcg ttggtcgtga ccgactggca cgtcttcgta
      181 aggagctaag gctacgctgt aagcagaaac gcaagttcag agcgactacg aacccgaacc
      241 acaatctgcc agttgcgcca aatctgctga accagacgtt cgctcctaca gcaccaaatc
      301 aggtctgggt ggcggacctg acgtatgttg ccacacagga gggatggttg tacctcgctg
      361 gcatcaaaga tgtttatacg tgcgaaattg tcggctacgc catgggagag cgcatgacaa
      421 aagagctgac aggtaaagcc ctgtttatgg cgctcaggag ccagcgccca cctgccgggc
      481 taatccacca ctctgatcga ggttcacagt actgcgcata cgattaccgg
//
LOCUS       DAJTSL010000358          525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTSL010000358 DAJTSL010000000
VERSION     DAJTSL010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.362"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            228..525
                     /locus_tag="OO433_004255"
                     /pseudo
     CDS             228..525
                     /locus_tag="OO433_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          151 a          128 c          133 g          113 t
ORIGIN      
        1 tgcgatctgg ttcaacaata tgcagatggt acacaacctc tttaaccagc ccatccggat
       61 tattcagagc ccttccgcaa tccggaataa aagtgctgtt ttaccgtgaa ccctctgaaa
      121 atcagcgagc agtgttttaa ccagccgacg ttcagagcgt actgacagac ggtggacaat
      181 ttgaataaac agttcaatca gaccgtagat gctctcccgt tggcgacatg ctgggagcgg
      241 tggaacaccg cttcggcaac gagcttccgg cgtctccagt gaagtgactg acggataatg
      301 gttcatgcta ccgggctaat gaaacacgcc agttcacccg gatgttggga ctttgttgaa
      361 ccgaagaaca cggcggtgcg gagtccggag agtaacagaa tagcaaagag cttcgtgaaa
      421 acaataaagt gtgactacat cagtatcatg cccaaacctg acgggttaac ggcagcaaag
      481 aaccttgcag aggcattcga gcattataac gaatggcatc cgcat
//
LOCUS       DAJTSL010000359          514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTSL010000359 DAJTSL010000000
VERSION     DAJTSL010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.363"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..279
                     /locus_tag="OO433_004256"
     CDS             <1..279
                     /locus_tag="OO433_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6052487.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          125 a          115 c          139 g          135 t
ORIGIN      
        1 agcctgaacg tgtactgtcg taatggccag gtggaaatcg acaacaacat cggtgaaaac
       61 gcgctgcgat cggtggcggt tggaagaaaa aattatctct tcttcggttc tgacaacggt
      121 ggagaaagcg cggcgattat ctacagtctg ctggtcacct gcaaactgaa cggagtggag
      181 ccggaggact ggttgcgcga agtgatcgtg aagctcaatg actggccgtc gaaccgagtg
      241 catgaactgc tgccctggaa cttctcagct gtaaaataat ccttccgcta cgtacttctc
      301 tgggcgctta ccactcaacg gcatcgtcaa aattatcaaa gcgcagaaca tagatttatc
      361 atgtgtttta tggatggctt tctccatcag gcatcgttcg tcacgggaaa ttggtgctat
      421 gatcggcatt gatcagtccg gttggtgatt tgttttgatt tggcgattga tcagatcgca
      481 caatccgggc tgagttccct ctaagtgatc tagg
//
LOCUS       DAJTSL010000360          483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTSL010000360 DAJTSL010000000
VERSION     DAJTSL010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.364"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(45..323)
                     /locus_tag="OO433_004257"
     CDS             complement(45..323)
                     /locus_tag="OO433_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052488.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
BASE COUNT          110 a          121 c          145 g          107 t
ORIGIN      
        1 gcccgagttc cggaaccaga acaggtcgaa gtaatacccg cacattacct ccagatccgt
       61 tgctggaatg tgcaggacgg acgcggtggc cgttcggaat aagggagcct gacgaagatt
      121 atccagtgac gataatcgag actgagggct ttcttaatct cgtatccgtg tctgcggtag
      181 cgctgaatca gccattcagc ctgttcttcg gtgcaggggt cgtgctgata ccagtcagat
      241 ttgaatgcat gagaacgccg cccgtgcctg ctggcaggcg cggcagagtt atccgaattg
      301 taaaatttgg tatcgtgcgc catctgtttt ctctgctggc gcagcaggtg ccagttgttc
      361 aggctgacgg atggattgta aaccagaacg accagaaaaa acaaaacccg ccgaagcggg
      421 ttaagtgcgg gtgcgttgag gatgcctgac acatcagcgg tggcgaggga tttctccccc
      481 gcc
//
LOCUS       DAJTSL010000361          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTSL010000361 DAJTSL010000000
VERSION     DAJTSL010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.365"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
BASE COUNT          170 a           81 c           77 g          148 t
ORIGIN      
        1 caaccatctt gccgtagaaa tcaactttat tgccatcttt attgtaaatt tcagccgcat
       61 ttgctgcgcc agcaactaat aacgccggga ccagcattgc cagaactttt cttttcatta
      121 tgtattccct tgtgattata atcttcatga atatatcaat aagtgccgtt atccaaaaaa
      181 aagcacattt ggatactatt ctatgaagtt catttcattt taagtattac atgcaacaaa
      241 tgtatttaaa agatttcaaa atgtttctaa tctatttaat ataattcaga gatatgaatt
      301 ataaatattc atgaaaatta taaatgtata tcaatatgtt atgagttgag cggaaacata
      361 gggggtatca atgattgttg cgccaaacgg ctggtgtgat caggcgcaca ttaatgaagg
      421 cataaaaaaa agccggcatc atgccggccc aaaaaatata aacaagacga taaaag
//
LOCUS       DAJTSL010000362          473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTSL010000362 DAJTSL010000000
VERSION     DAJTSL010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.366"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..333)
                     /locus_tag="OO433_004258"
     CDS             complement(<1..333)
                     /locus_tag="OO433_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6052489.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPES"
BASE COUNT          147 a          102 c          111 g          113 t
ORIGIN      
        1 actttccggt aaaaataaaa acggtaaata gagcgcaata tcggaaataa catcgccagt
       61 ttcattcaaa atcgccccca gacgtgtttg ctgattgcac tcacgcgcca acatgccatc
      121 cagcgcattg agcgccatac ggataaaaag cacgatgggc aatagcaaaa agaggatggg
      181 ttgtgccacc aacaccaaca gcaatccggt aaaaagagaa agcgccagtg ccgtaagagt
      241 gatgtgattc gctgtaacgt ggtgcttata aagccaaaac atcgtcggcc ttaacagcga
      301 ctgaaagagc ggttttatct ggtagagcgt catttatcat tccgtgatat tcagtttgct
      361 ctatggtgca tcaattgtga tggcggagat atcacaacgc ggagcatcaa cgtaaattcg
      421 gaatatatca agatgtggtg gatggttttc ctacacatct gtttgattac cga
//
LOCUS       DAJTSL010000363          467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTSL010000363 DAJTSL010000000
VERSION     DAJTSL010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.367"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>467)
                     /locus_tag="OO433_004259"
     CDS             complement(<1..>467)
                     /locus_tag="OO433_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR6052490.1"
                     /translation="KGGKDGITMTFIGPSPSVQDKSASTDNTNINGEDMTEIEESMLL
                     PVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARAEELPAVTALAISHKTSLLDSL
                     EIRDLHKLVRDTDKVFPNPGNSDLGLITAFFEAYLDADYTDRGLLTKEWMKGN"
BASE COUNT          104 a          107 c          125 g          131 t
ORIGIN      
        1 gatttccttt catccactct tttgtcagca ggccccgatc agtgtagtca gcgtccaagt
       61 atgcttcgaa aaaagcagtt attagtccca ggtctgaatt accaggatta gggaaaactt
      121 tgtcagtgtc acgaaccagt ttgtggaggt cgcgaatctc cagcgagtcg agcagactgg
      181 ttttatgcga aatagccagg gcagtaacag ccggtagttc ttcagcccgt gcaatgtgta
      241 atgcctggag ttcgtcgcgt gaaacgtgcg ttactggttt ttcgctgccg tgttgagcaa
      301 gccaacgaat gggcagttcc tgaccggaga caggtagaag catgctctcc tcaatctcag
      361 tcatgtcttc gccgttgatg ttggtattat cagtgctggc tgatttgtcc tgaacagagg
      421 gggaagggcc gataaatgtc attgtgatgc catctttccc gcctttt
//
LOCUS       DAJTSL010000364          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTSL010000364 DAJTSL010000000
VERSION     DAJTSL010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.368"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            35..447
                     /locus_tag="OO433_004260"
                     /pseudo
     CDS             35..447
                     /locus_tag="OO433_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          101 a          115 c          121 g          110 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacagtggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggacggttgt ggcgcac
//
LOCUS       DAJTSL010000365          436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTSL010000365 DAJTSL010000000
VERSION     DAJTSL010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.369"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(98..436)
                     /gene="fimH"
                     /locus_tag="OO433_004261"
                     /pseudo
     CDS             complement(98..436)
                     /gene="fimH"
                     /locus_tag="OO433_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
BASE COUNT          118 a          108 c          112 g           98 t
ORIGIN      
        1 agaagcagaa aaagcttatt atgcttccat cggaaacgat gatctggcag cctgggttca
       61 cagataaaac actctccagg aaacccgggg cggttcatac tgctcctaac gataccgtgt
      121 tattcgctgg aataatcgta ccgttgcgcg tcaactgtac gccgacgccc tgcgctggtg
      181 aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc tgcggttgtg ccggagaggt
      241 aatcccccag gttttggctt ttcgcacaat aaacggtaag aggaattggc actgaaccag
      301 ggtagtccgg cagagtaacg gtgacatcac gagcagaaac atcgcagccg ccagtaggca
      361 ccaccacatc attattggcg taaatattcc acacaaactg gaaatcatcg ctgttatagt
      421 tgttggtctg tcgcaa
//
LOCUS       DAJTSL010000366          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTSL010000366 DAJTSL010000000
VERSION     DAJTSL010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.370"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>431)
                     /locus_tag="OO433_004262"
     CDS             complement(<1..>431)
                     /locus_tag="OO433_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6052491.1"
                     /translation="AGWVNLLEDPKRIFLRQKNNLSDLQNKGTVRDNLQVYSKEQSDQ
                     RYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEGQGENGDIGPL
                     RPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNS"
BASE COUNT          107 a          128 c           88 g          108 t
ORIGIN      
        1 gaattccccc ccagggcgtt ctgaacgccg atacccagcg caccattgac ctgcgaacca
       61 ccgccaacag acactttatg cgacatggat atttcacccg tccgcaaatt aatggtgaac
      121 gggcgcaggg gaccaatatc gccattttcc ccctgacctt cactggtggg aataaggtgc
      181 aggcactctt ccgaacgacg aaaaatcagg ccaaaagctt cgttgaaaat cctcagcgca
      241 ttaacaccac ggattttcag ctctccggtt attttatcgc cttcccgatg aacatagcgt
      301 tgatctgact gctctttact gtaaacctga agattatccc gtaccgtccc tttattctga
      361 aggtctgaca gattgttctt ctgccgcagg aatatccttt tcggatcctc aagaagattt
      421 acccagcccg c
//
LOCUS       DAJTSL010000367          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTSL010000367 DAJTSL010000000
VERSION     DAJTSL010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.371"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            143..431
                     /locus_tag="OO433_004263"
                     /pseudo
     CDS             143..431
                     /locus_tag="OO433_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          143 a           61 c           72 g          155 t
ORIGIN      
        1 tttttatagt acttcacttc ttcttcacta agaatgtcca ttatctcaat gccttatcag
       61 agattctttt cctttcgccg gtagtgtctg gacgctcagg ctacttttcc aggttatttt
      121 atttctgtat tacagaggtt ttatgatagg tcatatcaca aattctggtg gcaataactc
      181 tttgatgaaa catgatgtgg ttcaaggaaa taatatagta gatcttgatt tactacgtaa
      241 tttcaatggg gtgacaggtt taaacagaga taactttatt tatatcagta atatattttt
      301 aaatataaaa caacggaacg aaaaaatcat gcaataaata tgttacgtga agtctcaatt
      361 agtaatgata ctataagtgt aaaattctac agaaatgaaa aaatagaatg cgcttgtaat
      421 tttctgatgg a
//
LOCUS       DAJTSL010000368          426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTSL010000368 DAJTSL010000000
VERSION     DAJTSL010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.372"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>426
                     /gene="fsr"
                     /locus_tag="OO433_004264"
     CDS             <1..>426
                     /gene="fsr"
                     /locus_tag="OO433_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6052492.1"
                     /translation="YYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIGR
                     KYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIGM
                     VSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFL"
BASE COUNT           74 a          100 c          114 g          138 t
ORIGIN      
        1 gctattacac cttttatctg atgcaaaaat tcggattatc tatccagaat gcccagcttc
       61 atctgtttgc cttcctgttt gccgttgcgg caggtacggt gatcggcggg cctgtagggg
      121 ataaaattgg acggaaatat gtgatttggg gctctatcct cggcgttgcg ccgtttacgc
      181 tgattttacc ctacgccagc ctgcactgga cgggggtttt aacggtgatt attggattta
      241 tcctcgcttc ggcattctct gccattctgg tctacgctca ggagctactt ccgggacgta
      301 tcggtatggt ttctggactc tttttcggtt ttgcttttgg catgggaggt ctgggagcgg
      361 cagttctggg gcttatcgcc gatcacacca gcatcgagtt agtctataaa atctgtgctt
      421 tcctgc
//
LOCUS       DAJTSL010000369          425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTSL010000369 DAJTSL010000000
VERSION     DAJTSL010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.373"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
BASE COUNT          166 a           69 c           79 g          111 t
ORIGIN      
        1 gactctatca tcgccaatca tcagacattg aaatagacga cactgagatg acgcttacaa
       61 ttcttttggt ttctgtaata agtaatctga catcccctgt gcccagaaga ggagagggat
      121 atcaagccca agggttaata attgatacta cggttaaaat tgccttcagt aaaggaaacg
      181 tgattaaatt caggggattt cagagtgaaa accatacaac atattaaaat ctgttcattt
      241 agctataata catacaagaa agtgtccagt tagataatgt tatctaaata accacagata
      301 aaaacgcaca taattggggg ggggatgaaa atacctgaag cagtaaatca tattaatgtt
      361 cagaataata ttgatcttgt tgatggaaaa acgaatccga ataaagcaac aaaagcatta
      421 caaaa
//
LOCUS       DAJTSL010000370          419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTSL010000370 DAJTSL010000000
VERSION     DAJTSL010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.374"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            243..>419
                     /locus_tag="OO433_004265"
     CDS             243..>419
                     /locus_tag="OO433_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052493.1"
                     /translation="MKTLNRRDIPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFP"
BASE COUNT          109 a           96 c           89 g          125 t
ORIGIN      
        1 tgaggaagtt gtctgacatc tttattcatt ccgcgcgatt tgaatgcaat gtgttctacc
       61 actttatgat ccaggaaaag gcgtatttcc tcgattaaac catgatccgc gccacacttt
      121 tctcataaaa ctgctcatta atcatcagga gttgatcaaa acaggggttc ggcgtattaa
      181 attttgctaa cctgtcgtac caattcagac gggttgtatg actaatcaga agggaaccca
      241 ttgtgaaaac actaaatcgt cgcgatattc ccggtgcaca gtatccagaa cgtatcattc
      301 agtttggtga aggtaacttc ctgcgcgcct ttgttgactg gcaaatcgat ctcctgaatg
      361 agcacaccga tctgaattct ggcgtggtcg ttgttcgtcc gattgaaact tcattcccg
//
LOCUS       DAJTSL010000371          413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTSL010000371 DAJTSL010000000
VERSION     DAJTSL010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.375"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..63)
                     /locus_tag="OO433_004266"
                     /pseudo
     CDS             complement(1..63)
                     /locus_tag="OO433_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
     gene            complement(61..413)
                     /locus_tag="OO433_004267"
                     /pseudo
     CDS             complement(61..413)
                     /locus_tag="OO433_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           95 a          103 c          107 g          108 t
ORIGIN      
        1 gaatcggaaa atcgatactg gaaaaactct aactcggtgg ccagtttttg ttgaccactt
       61 catcccgagg ttcaccatct gcttaaagaa gttgctcgag aagaaaacat agatcgagtc
      121 tgcgctcttc atctcgttga aggtgtagta ataggcggta ttgctcaccg gtttccatga
      181 actgccattc agactgaact gcatatcgta cgccccgacg gccgaggcta acgacgagtt
      241 ggcgatcgcc gggaatatat ggattgaggt gttgctgagt ccgtttgttt gcagggtata
      301 gttgaccatc ttacaactta atcctgaaag gctgccgatg gtctgtgtcc ggcagtcggc
      361 gttcccttcg cccagggtcg gcaccccgtc gctgttgata aatacctccg cca
//
LOCUS       DAJTSL010000372          403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTSL010000372 DAJTSL010000000
VERSION     DAJTSL010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.377"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>403)
                     /locus_tag="OO433_004268"
     CDS             complement(<1..>403)
                     /locus_tag="OO433_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR6052494.1"
                     /translation="ATQKMLDDAFFNVEAWINSELATEFAEQEEIAFTTGDGTKKPKG
                     FLANESTDETDKVRAFGKLQHIVSGDATAVTADAIIKLIYTLRKAHRTGAKFMMNNNS
                     LFAIRLLKDSEGNYLWRPGLELGQPSSLAGYG"
BASE COUNT           77 a          118 c          105 g          103 t
ORIGIN      
        1 ccgtaacccg ccagagagga cggctgcccc agctccagcc ccggacgcca cagatagtta
       61 ccctcgctgt ctttcagcag acggatggca aacaggctat tgttgttcat catgaacttc
      121 gcgccggtgc ggtgtgcctt acgcagcgtg taaatcagtt tgataatggc gtctgcggtc
      181 accgccgtcg cgtcgccgga tacaatatgc tgaagtttgc cgaacgcccg gaccttatcg
      241 gtttcatccg tggattcatt cgccaggaac cctttcggct tcttggtacc atcgccggtg
      301 gtaaaggcaa tttcttcctg ttcggcaaat tcggttgcca gctcgctgtt gatccatgct
      361 tccacgttga aaaaggcatc atccagcatt ttctgggtgg cct
//
LOCUS       DAJTSL010000373          393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTSL010000373 DAJTSL010000000
VERSION     DAJTSL010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.378"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>393
                     /locus_tag="OO433_004269"
     CDS             <1..>393
                     /locus_tag="OO433_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6052495.1"
                     /translation="ETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLAGMKLGDTPLV
                     DFTRDRLHRETLRRFGRYELSTAYTPAGQLQSQHLNSLQYDRDYTWNDNGELIRISSP
                     RQTRSYSYSTTGRLTGVHTTAANLDIRI"
BASE COUNT           88 a          140 c          108 g           57 t
ORIGIN      
        1 atgagaccag acatgcgtac aacgcgcagg gactggcgaa ccgctgtata ccggacagcc
       61 tgcccgccgt ggaatggctg acctacggca gcggctggct ggcaggtatg aagctcggcg
      121 acacaccgct ggtggatttc acccgcgacc gcctgcaccg ggaaacgctg cgccgcttcg
      181 gccgttatga actctccacc gcttacaccc ctgccgggca gttacagagc cagcacctga
      241 acagcctgca gtatgaccgc gattacacct ggaacgacaa cggcgaactc atccgcatca
      301 gcagcccgcg ccagacccgg agttacagct acagcaccac cggcaggctg accggcgttc
      361 acaccaccgc agcgaacctt gatatccgca tcc
//
LOCUS       DAJTSL010000374          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTSL010000374 DAJTSL010000000
VERSION     DAJTSL010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.381"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..355)
                     /locus_tag="OO433_004270"
     CDS             complement(<1..355)
                     /locus_tag="OO433_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6052496.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKQARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIK"
BASE COUNT           89 a           82 c           90 g          104 t
ORIGIN      
        1 gcttaatttt gctttcacat ttttgtaagt cttctgccgg attgtgcgac gtctgcaata
       61 tcacgtcgaa accttcctct tcagctttgg cggtgatggc atgcaaaact tcagagaaaa
      121 acggattacc cgccgtagtt ttggtcgaac gggtagaaat caccataatg gcatcaaaac
      181 ccgaagaggt cagcgcgcgg gcctgcttgt ttggctgata ctgtaattct tcaatggccc
      241 gtaacacttt ttcgcgcgct tccggggaga tattagtttg cttattcagc acgcgtgata
      301 cggtggattt tgatacgcct gcaaccctgg caatatcata aatagtaggg gacataggtc
      361 aggaa
//
LOCUS       DAJTSL010000375          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTSL010000375 DAJTSL010000000
VERSION     DAJTSL010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.383"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            84..>364
                     /locus_tag="OO433_004271"
     CDS             84..>364
                     /locus_tag="OO433_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6052497.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFRN
                     AGLSWPLPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRRP"
BASE COUNT           81 a          104 c           85 g           94 t
ORIGIN      
        1 taaccggctc atttaaaccg tctggtctgt ttcctccggc tccacaaaaa taatgtccat
       61 cattttgaag tggacactat cgtatggaat accggacctg gatcactgaa gctttacgcc
      121 ttcatttcga agaacattta ccacgggttg tggccggacg tcgcctgggc gtaccaaaat
      181 caactgtctg tggtatgttc gtgcgcttcc gcaacgctgg cctttcatgg cctctgcccg
      241 caggtatgtc ggagcaggaa cttgatgccc ttctttacgg aagtgcctcc acagtacctg
      301 tcgtacttac tgaaagcacc gttatgccca aactcccggt ggtaaaaaaa cgtccccggc
      361 ggcc
//
LOCUS       DAJTSL010000376          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTSL010000376 DAJTSL010000000
VERSION     DAJTSL010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.384"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>361
                     /locus_tag="OO433_004272"
     CDS             <1..>361
                     /locus_tag="OO433_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR6052498.1"
                     /translation="KQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHL
                     KGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPR
                     VVKERPWKYPTAPKKSQS"
BASE COUNT          108 a           71 c          111 g           71 t
ORIGIN      
        1 aaacagacga tgcaactgag caggctgacg ctgagaagta aaaagccgga gcttgtggag
       61 caagagctgt ggggtgtctt actggcttat aatctggtga gatatcagat gattaaaatg
      121 gcggaacatc tgaaaggtta ctggccgaat caactgagtt tctcagaatc atgcggaatg
      181 gtgatgagaa tgctgatgac attgcagggc gcttcaccgg gacgtatacc ggagctgatg
      241 cgcgatcttg caagtatggg acaacttgtg aaattaccga cgagaagggg aagggccttc
      301 ccgagagtgg taaaggagag gccctggaaa taccccacag ccccgaaaaa gagccagtca
      361 g
//
LOCUS       DAJTSL010000377          355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTSL010000377 DAJTSL010000000
VERSION     DAJTSL010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.385"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..342
                     /locus_tag="OO433_004273"
     CDS             <1..342
                     /locus_tag="OO433_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HCR6052499.1"
                     /translation="LGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGG
                     FYLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQ
                     IQLTQCTRCVK"
BASE COUNT           95 a           81 c          103 g           76 t
ORIGIN      
        1 cttggcccaa cgaccgccgg acggatggat tcttatgtcg atcaactgca agcgcagggc
       61 ggaagtatga tcatgctggc gaaaggcaac cgcagccagc aggtgacgga tgcctgtaaa
      121 aaacacggcg gcttctacct tggcagtatc ggtggtccgg ccgctgtatt ggcgcaggga
      181 agtattaaga gcctggaatg tgttgaatat ccggaactgg gaatggaagc catctggaaa
      241 attgaagtgg aagatttccc ggcgtttatc cttgtggatg ataaaggaaa tgacttcttc
      301 cagcagatac aactcacaca gtgcactcgc tgtgtgaaat aaacagagcc gccct
//
LOCUS       DAJTSL010000378          350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTSL010000378 DAJTSL010000000
VERSION     DAJTSL010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.386"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            27..350
                     /locus_tag="OO433_004274"
                     /pseudo
     CDS             27..350
                     /locus_tag="OO433_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155249.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT           69 a           92 c           79 g          110 t
ORIGIN      
        1 acaccgttga cgtaaaaaag aaaggcgtgc atacccgcta tttaatatta ctgattattt
       61 ttattgttac cgccgttaac tacgccgatt gtgcaacgct gtctattgct ggtaccgaag
      121 tcctacctgc tgatgcaaat tcccggcggc tggctgcttg ataagtttgg ctcgaaaaaa
      181 gtttacacct acagcctctt tttctggtcg ctattcacct tcctgcaagg ctttgttgat
      241 atgttcccgc tggcctgggc agggatctct atgttcttta tgcgctttat gctcggcttc
      301 tcggaagcgc catcattccc ggcgaacgcc cgtattgtcg ccgcctggtt
//
LOCUS       DAJTSL010000379          342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTSL010000379 DAJTSL010000000
VERSION     DAJTSL010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.387"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..225)
                     /locus_tag="OO433_004275"
     CDS             complement(<1..225)
                     /locus_tag="OO433_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCR6052500.1"
                     /translation="MTWNLLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRN"
BASE COUNT          118 a           56 c           51 g          117 t
ORIGIN      
        1 atttcttaat aagaaggtat taaattcatc tggattgctg atactactta ccggacaaat
       61 aaaggtttcg ataatcattt ggcgggaagt aaaaagaata ttgatttgca aatcgccata
      121 atcattcatt ttaataatta atgcgttctc gctatttgtt acatcaatat tttgttcagg
      181 tacagtttgc agcgccgtcg ccagcgccaa cagattccat gtcatatatt attccatata
      241 gatttagttt aaatattaat aaaatgaata tttgcagtac gtaattatct taccagctat
      301 agacaaaaaa aaaccatcca aatctggatg gcttttcata at
//
LOCUS       DAJTSL010000380          333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTSL010000380 DAJTSL010000000
VERSION     DAJTSL010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.388"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..289)
                     /locus_tag="OO433_004276"
     CDS             complement(<1..289)
                     /locus_tag="OO433_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6052501.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPS"
BASE COUNT           94 a           80 c           95 g           64 t
ORIGIN      
        1 cactgggggc aacgaagggg cgattgcccg gcagcatgat gtccaggcga ttcacaatct
       61 ggtgaagagg ttctttacgc tcaagcgcca tgccaacaat acaccagacc atcatttcga
      121 ggggaagacg gcgcttgcgt agcgttacag tacctgattc ggcaaggcaa cgagagatga
      181 gttcggggtc gaggtaatcc cccagagaag tcagtgggtt acgcagagaa tcgtaacggg
      241 ataccagatc aagagcctgt ccaatgtgca taaaaaaatc cggaaacaag tgagcgtttc
      301 cggattctta cacagccact ggatcggtca act
//
LOCUS       DAJTSL010000381          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTSL010000381 DAJTSL010000000
VERSION     DAJTSL010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.389"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..301)
                     /gene="eamB"
                     /locus_tag="OO433_004277"
     CDS             complement(<1..301)
                     /gene="eamB"
                     /locus_tag="OO433_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6052502.1"
                     /translation="MTPTLLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGL"
BASE COUNT           87 a           87 c           80 g           77 t
ORIGIN      
        1 gaagtccgtc ttcctttgtt gggctggtgg cgattttcca cgccagccag acaatatatg
       61 ccgcccccgc ccaactcaaa aggtgtaccg ctgccgggtc aatcactgcc agtgaaaatg
      121 aaatgcccgc acacagtaac atcacaatca aaaatcccag actcatccct gccagcacgc
      181 gggtactttg acgaaatcca tgcgacgtag cagagctaag ggcgagaata ttgttcggtc
      241 ttggcgtcat agcggtaatc agggtgtaag tccaaaaagc acttaaaagg gtcggtgtca
      301 cttgttatct cctctgtttt ggtagacagt a
//
LOCUS       DAJTSL010000382          326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTSL010000382 DAJTSL010000000
VERSION     DAJTSL010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.391"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>326
                     /locus_tag="OO433_004278"
     CDS             <1..>326
                     /locus_tag="OO433_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6052503.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEA"
BASE COUNT           74 a           85 c           97 g           70 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagc
//
LOCUS       DAJTSL010000383          315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTSL010000383 DAJTSL010000000
VERSION     DAJTSL010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.393"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            88..>315
                     /gene="ytfB"
                     /locus_tag="OO433_004279"
     CDS             88..>315
                     /gene="ytfB"
                     /locus_tag="OO433_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCR6052504.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTE"
BASE COUNT           79 a           81 c           87 g           68 t
ORIGIN      
        1 aataaaggcg ggtttaccac ggacatcata gcgttagctt attcataggc tatgattgag
       61 gaacaagacg cggagcagga ggaaaccatg cccgggcgct ttgaactaaa accaaccctg
      121 gagaaagtct ggcacgcgcc ggacaatttt cgcttcatgg acccgctgcc gccgatgcat
      181 cgtcggggga tcattattgc cgccattgta ttggtggtcg gatttctgct tccatctgat
      241 gatacgccca acgcgccggt ggtgacgcgt gaagcgcagt tagatattca gtcgcaatca
      301 caaccgccga cagaa
//
LOCUS       DAJTSL010000384          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTSL010000384 DAJTSL010000000
VERSION     DAJTSL010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.denovo.397"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>304
                     /locus_tag="OO433_004280"
     CDS             <1..>304
                     /locus_tag="OO433_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR6052505.1"
                     /translation="VIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDYLRTRP
                     VDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSK"
BASE COUNT           95 a           58 c           61 g           90 t
ORIGIN      
        1 ggtgatcatt atggatactc atcctatcat cagaatgtct attgaagttc tgttgcaaaa
       61 aaacagtgaa ttgcagattg tcctgaaaac ggatgattac cgcataacca tcgattatct
      121 ccgaacccgt cctgttgatt taatcattat ggatatagac ttgcccggaa cagacggttt
      181 taccttcctg aaaaggatca aacaaatcca gagcacagtg aaagtgttat ttttatcatc
      241 gaaatcagaa tgcttttatg ctggcagagc gatacaagct ggtgctaacg gttttgtcag
      301 taaa
//
LOCUS       DAJTSL010000385         7553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.guided.180, whole genome shotgun sequence.
ACCESSION   DAJTSL010000385 DAJTSL010000000
VERSION     DAJTSL010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.guided.180"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(37..603)
                     /locus_tag="OO433_004281"
                     /pseudo
     CDS             complement(37..603)
                     /locus_tag="OO433_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(822..1799)
                     /gene="epmA"
                     /locus_tag="OO433_004282"
     CDS             complement(822..1799)
                     /gene="epmA"
                     /locus_tag="OO433_004282"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6052506.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2124..3932
                     /gene="frdA"
                     /locus_tag="OO433_004283"
     CDS             2124..3932
                     /gene="frdA"
                     /locus_tag="OO433_004283"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6052507.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3925..4659
                     /gene="frdB"
                     /locus_tag="OO433_004284"
     CDS             3925..4659
                     /gene="frdB"
                     /locus_tag="OO433_004284"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6052508.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4670..5065
                     /gene="frdC"
                     /locus_tag="OO433_004285"
     CDS             4670..5065
                     /gene="frdC"
                     /locus_tag="OO433_004285"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6052509.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5076..5435
                     /gene="frdD"
                     /locus_tag="OO433_004286"
     CDS             5076..5435
                     /gene="frdD"
                     /locus_tag="OO433_004286"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6052510.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5498..6631
                     /locus_tag="OO433_004287"
     CDS             5498..6631
                     /locus_tag="OO433_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6052511.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6720..7253
                     /gene="blc"
                     /locus_tag="OO433_004288"
     CDS             6720..7253
                     /gene="blc"
                     /locus_tag="OO433_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6052512.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7250..>7553)
                     /gene="sugE"
                     /locus_tag="OO433_004289"
     CDS             complement(7250..>7553)
                     /gene="sugE"
                     /locus_tag="OO433_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6052513.1"
                     /translation="LVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMALLAWA
                     MKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKLSTH"
BASE COUNT         1939 a         1972 c         1920 g         1722 t
ORIGIN      
        1 atcatacact aaatcagtaa gttggcagca ttacccgaaa tgcccaccat ttaataacaa
       61 aatagtaatg cttactaaca gcaatactaa attcagacac attactgcaa taccgcccac
      121 cgcagtaatg cgggcaattt tattaatccc ctttgaagca acgacggtga ccagaatcat
      181 ccatgctact gccagcagac caaccacctg cgtaggctcc agtccggcaa tacgccagtg
      241 ctgggtcatg tcgctaccat agaggaatgt tgagaacggt acccaaactt tcgcggaggt
      301 actcaccatc cagatgatat aagaggaaaa ccacataaac gtaccaatga aggcaaaacg
      361 tggtccgaca ctattattca tccaggaata gataccacct tcttctttgc gataagcagc
      421 tcccatttca gccatcatta aggcgaatgg aataaagaat aacaatgcag aaaatatata
      481 aaagggaatc gcactataac ccattaagta ataagccgat gggctattgg caaatccaaa
      541 tacggaagta aagatcatca atatgagtcc tatcagactc atctttttta tcgtgtgagg
      601 catgaaacca tccttcattt cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc
      661 aacattgagc aggcagcgta aaaaccaata cagaattgat acccggatga tatcaaatat
      721 ccagtcttaa attgtggcct ttaacggaaa aatgaaaaca tatgctacag aatggcgctt
      781 atcacgccat tcttcgctgt taattcagta atttttcaga attatgcccg gtcaacgcta
      841 aaggcgatga cttcagccag tgtctccgcg cccagcgcca acatcaccag acgatcaaca
      901 cctaatgcca cgccggaaca gtcaggcata ccgactttca aggcttcaat cagattctgg
      961 tcaatggggt gctgcggcaa accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt
     1021 tgttgctgct cacgggcatc cgttaattca tggaaaccat tcgccagctc aataccttta
     1081 taataaacct caaagcgttc agcgacccga tgatcttcgg tactgatttg cgccagtgat
     1141 gcctggctgg ctggaaagtg gtacacaaag gtcggttttt ctttgccaat atttggctct
     1201 acgccaaagg taaacagcaa ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg
     1261 ttactcaaat ccagtttcgc tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg
     1321 tcaatttcca gataacgcaa gaaagcttgt tgataagaaa ggctttctgc tgccgggcag
     1381 tccagcacct gttgtaagag atcgtccacc tcgttcatca accggtacat atcatagtgc
     1441 ggtcgatacc actcaagcat agtgaactca gggttgtgat aacgccccat ctcttcatta
     1501 cggaagctgc ggcacagctg gaataccggc ccacaaccgg caaccagcag gcgtttcata
     1561 tggtattccg ggctggtcat taaccagaga ttcatcccct gcgaatgccc ggggccaacg
     1621 aaacgtgtct caaacgggac caaatgaata tcggttaccg tcgcctggct catacaaggc
     1681 gtctccacct ccagcactcc acgatcagca aagaaacgac ggatctccgc cataatcgcc
     1741 gcgcgtttta ataagttagg aatggatgcg ctcggctgcc aggatgccgt ttcgctcata
     1801 gttaaatctc cagtttttga caagggcacg aagtctactc gcaacgcgac ggcgagacaa
     1861 attttacgca ggaatcaaac agcggtgggc agtgactaaa aaaagcacga tctgatggtt
     1921 tagtaattaa attaatcatc ttcagtgata atttagccct cttgcgcact aaaaaaatcg
     1981 atctcgtcaa atttcagact tatccatcag actatactgt tgtacctata aaggagcagt
     2041 ggaatagcgt tcgcagaccg taactttcag gcacttaccc tgaagtacgg ggctgtggga
     2101 taaaaacaat ctggaggaat gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg
     2161 ccggtggcgc gggattacgt gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa
     2221 tcgcactaat ctcaaaagta tacccgatgc gtagccatac cgttgctgca gaagggggct
     2281 ccgccgctgt cgcgcaggat catgacagct tcgaatatca ctttcacgat acagtagcgg
     2341 gtggcgactg gttgtgtgag caggatgtcg tggattattt cgtccaccac tgcccaaccg
     2401 aaatgaccca actggaactg tgggggtgcc catggagccg tcgcccggat ggtagcgtca
     2461 acgtacgtcg cttcggcggc atgaaaatcg agcgcacctg gttcgccgcc gataagaccg
     2521 gcttccatat gctgcacacg ctgttccaga cctctctgca attcccgcag atccagcgtt
     2581 ttgacgaaca tttcgtgctg gatattctgg ttgatgatgg tcatgttcgc ggcctggtag
     2641 caatgaacat gatggaaggc acgctggtgc agatccgtgc taacgcggtc gttatggcta
     2701 ctggcggtgc gggtcgcgtt tatcgttaca acaccaacgg cggcatcgtt accggtgacg
     2761 gtatgggtat ggcgctaagc cacggcgttc cgctgcgtga catggaattc gttcagtatc
     2821 acccaaccgg tctgccaggt tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg
     2881 gtattctggt caacaaaaat ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa
     2941 ctccgctggg cgagccgaaa aacaaatata tggaactggg tccacgcgac aaagtttctc
     3001 aggccttctg gcacgaatgg cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg
     3061 tttatctcga cttgcgtcac ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct
     3121 gcgaactggc gaaagcgtac gttggcgtcg atccggttaa agaaccgatt ccggtacgtc
     3181 cgaccgcaca ctacaccatg ggcggtatcg aaaccgatca gaactgtgaa acccgcatta
     3241 aaggtctgtt cgccgtgggt gaatgttcct ctgttggtct gcacggtgca aaccgtctgg
     3301 gctccaactc cctggcggaa ctggtggtct tcggccgtat ggccggtgaa caagcgacag
     3361 agcgtgcagc aactgccggt aatggcaacg aagaggcaat tgaagcgcag gcagctggcg
     3421 ttgaacaacg tctgaaagat ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc
     3481 gcgacgaaat gggcctagca atggaagaag gttgcggtat ctaccgtacg ccggaactga
     3541 tgcagaaaac catcgacaag ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca
     3601 ccgacacctc cagcgtgttc aacaccgacc tgctctacac cattgaactg ggccacggtc
     3661 tgaacgttgc tgaatgtatg gcgcactccg caatggcacg taaagagtcc cgcggcgcac
     3721 accagcgtct ggacgaaggt tgcaccgagc gtgacgacgt caacttcctc aaacacaccc
     3781 tcgccttccg cgatgctgat ggcacgactc gcctggagta cagcgacgtg aagattacta
     3841 cgctgccgcc agctaaacgc gtttacggtg gcgaagcgga tgcagccgat aaggcggaag
     3901 cagccaataa gaaggagaag gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg
     3961 cgctataacc cggaagtcga taccgcaccg catagcgcat tctatgaagt gccttatgac
     4021 gcaactacct cattactgga tgcgctgggc tacatcaaag acaacctggc accggacctg
     4081 agttaccgct ggtcctgccg tatggcgatt tgtggttcct gcggcatgat ggttaacaac
     4141 gtgccaaaac tggcatgtaa aaccttcctg cgtgattaca ccgacggtat gaaggttgaa
     4201 gcgttagcta acttcccgat tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa
     4261 agtctggaag cgatcaaacc gtacatcatc ggcaactccc gcaccgcgga tcagggtact
     4321 aacatccaga ccccggcgca gatggcaaag tatcaccagt tctccggttg catcaactgt
     4381 ggtctgtgct acgccgcgtg cccgcagttt ggcctgaacc cagagttcat cggtccggct
     4441 gccattacgc tggcgcatcg ttataacgaa gatagccgcg accacggtaa gaaggagcgt
     4501 atggcgcagt tgaacagcca gaacggcgta tggagctgta ctttcgtggg ctattgctcc
     4561 gaagtctgcc cgaaacacgt cgatccggct gcggccattc agcagggcaa agtagaaagt
     4621 tcgaaagact ttcttatcgc gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa
     4681 acgtaaaccg tatgtacggc caatgacgtc cacctggtgg aaaaaattgc cgttttatcg
     4741 cttttacatg ctgcgcgaag gcacggcggt tccggctgtg tggttcagca ttgaactgat
     4801 tttcgggctg tttgccctga aaaatggccc ggacgcctgg gcgggattcg tcgacttttt
     4861 acaaaacccg gttatcgtga tcattaacct gatcactctg gcggcagctc tgctgcacac
     4921 caaaacctgg tttgaactgg caccgaaagc ggccaatatc attgtaaaag acgaaaaaat
     4981 gggaccagag ccaattatca aaagtctctg ggcggtaact gtggttgcca ccatcgtaat
     5041 cctgtttgtt gccctgtact ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt
     5101 ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg tatgtggagc gcaattattg
     5161 cgccggtgat gatcctgctg gtgggtattc tgctgccact ggggctgttt ccgggtgatg
     5221 cgctgagcta cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc gtattcctgt
     5281 tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg
     5341 atctgaaaat tcacgtgcct gcgggcaaat gggttttcta cggtctggct gctatcctga
     5401 cagttgtcac gctgattggt atcgttacaa tctaacgtat cgccaatgta aatccggccc
     5461 gcctatggcg ggccgttttg tatggaaacc agaccttatg ttcaaaacga cgctctgcgc
     5521 cttattaatt accgcctctt gctccacatt tgctgcccct caacaaatca acgatattgt
     5581 gcatcgcaca attaccccgc ttatagagca acaaaagatc cccggtatgg cggtggcggt
     5641 aatttatcag ggtaaacctt attactttac ctggggctat gcggacatcg ccaaaaagca
     5701 gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt
     5761 gcttggtggc gatgctattg ctcgagggga aatcaagtta agcgatcccg caacaaaata
     5821 ctggcctgaa cttaccgcta aacagtggaa tgggatcaca ctattacatc ttgcgaccta
     5881 caccgctggc ggcctgccat tgcagatgcc ggatgaagtg aaatcctcaa gcgacttgct
     5941 gcgcttctat caaaactggc agcctgcatg ggctccagga acacaacgtc tgtatgccaa
     6001 ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg tctggtttga gttttgagca
     6061 ggcgatgcaa actcgtgtct tccagccact caaactcaac catacgtgga ttaatgtacc
     6121 gcccgcagaa gaaaagaatt acgcctgggg atatcgcgaa ggcaaggcag ttcatgtttc
     6181 gccaggggcg ttagatgctg aagcttatgg tgtgaagtcg accattgaag atatggcccg
     6241 ctgggtgcaa agcaatttaa ccccccttga tatcaatgag aaaacgcttc aacaagggat
     6301 acaactggca caatctcgct actggcaaac cggcgatatg tatcagggcc tgggctggga
     6361 aatgctggac tggccggtca atcctgacag catcattaac ggcagtgaca ataaaattgc
     6421 actggcagca cgcccggtaa aagcgattaa gcccccaact cctgcagtac gcgcatcatg
     6481 ggtacataaa acgggggcga ccggcggatt tggtagctat gtcgcgttta ttccagaaaa
     6541 agagctgggt atcgtgatgc tggctaacaa aaactatccc aatccagcga gagtcgccgc
     6601 cgcctgtcag attctcaacg ctctacagta aaatttcatc gggtccgaat tttcggacct
     6661 tttctgcgct tttccttgct gtcatctaca cttagaaaaa aaccagtaag gaaacattta
     6721 tgcgcctgct ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt
     6781 ctcctacgcc gccgcgtggc gtgacagtgg tgaataattt cgacgctaaa cgctatctcg
     6841 gtacctggta tgagattgcc cgttttgatc accgttttga acgtggactg gaaaaagtca
     6901 ccgcaacata cagtctgcgt gatggcggcg gcctgaatgt catcaataaa ggctataacc
     6961 ctgacagaga aatgtggcag cagagtgaag ggaaagcgta ctttaccggc gcaccaacac
     7021 gcgctgcgct gaaagtgtca ttctttggtc ctttctatgg cggttataac gtcattgcac
     7081 ttgatcggga ataccgccat gcgctggttt gcggcccgga ccgcgactac ctgtggatac
     7141 tctcccgcac gccaaccatt tctgacgaag tgaaacagga gatgctggca gtcgcgaccc
     7201 gggaagggtt tgatgtcagt aagtttattt gggtacagca gcccggtagt tagtgagtgc
     7261 tgagtttcag accaataatc cccaatacga ttaacgccag actcgccagg cgcatcgggt
     7321 tagcggactc accgagcagc acaatgccgg tgatagccgc gccgactgcg ccaatacccg
     7381 tccacacggc ataagccgtc cctactggta acgatttcat cgcccaggca agtagcgcca
     7441 tactgacaat catcgccgtc acggtaataa cactcggcgt caaacgacta aagccgtggg
     7501 tatatttcag gccaacggcc cataccactt ccagcagacc agcaataact aag
//
LOCUS       DAJTSL010000386         6428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.guided.199, whole genome shotgun sequence.
ACCESSION   DAJTSL010000386 DAJTSL010000000
VERSION     DAJTSL010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.guided.199"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            607..1422
                     /gene="sul2"
                     /locus_tag="OO433_004290"
     CDS             607..1422
                     /gene="sul2"
                     /locus_tag="OO433_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6052514.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1483..2010
                     /locus_tag="OO433_004291"
                     /pseudo
     CDS             1483..2010
                     /locus_tag="OO433_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2031..2504
                     /locus_tag="OO433_004292"
     CDS             2031..2504
                     /locus_tag="OO433_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR6052515.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2579..2854
                     /locus_tag="OO433_004293"
                     /pseudo
     CDS             2579..2854
                     /locus_tag="OO433_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            2854..3690
                     /locus_tag="OO433_004294"
     CDS             2854..3690
                     /locus_tag="OO433_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6052516.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3797..4201)
                     /locus_tag="OO433_004295"
                     /pseudo
     CDS             complement(3797..4201)
                     /locus_tag="OO433_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4245..4634)
                     /locus_tag="OO433_004296"
     CDS             complement(4245..4634)
                     /locus_tag="OO433_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052517.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4852..5292
                     /locus_tag="OO433_004297"
     CDS             4852..5292
                     /locus_tag="OO433_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6052518.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(6048..6197)
                     /locus_tag="OO433_004298"
     CDS             complement(6048..6197)
                     /locus_tag="OO433_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6052519.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1595 a         1664 c         1663 g         1506 t
ORIGIN      
        1 cccgttcagt ccgaccgctg cgccttgtcc ggtaactatc aacttgagtc caacccggaa
       61 agacacgaca aaacgccact ggcagcagcc attagtaaca ggagtgcacg agaagataga
      121 ttaagaggtc ttgaagtaga ggcctgactg cggctacact gaaagaacag ttttggtaat
      181 ggcggtctcg tgaaccctgt taccgggtta agcagttccc caactgactt aaccttcgaa
      241 caaaccgcct ccccaggcgg ttttttcgtt tacagagcaa gagattacga ttcattgaaa
      301 aggatctcaa gaagatcctt tgtagattac tatcaattaa tcttccgaac atgacaccag
      361 tcactgttga ttacggttaa accgctccgt aacaggtttc cctcgtattg gtgacttgca
      421 gcaccaaaga agcaaaaaag ataaatatat ctacttattg agagatgata tatttatttc
      481 caaaatgcta cagatacatc atcatgaaac ggatcaccgc aaacaggtta ctcgcctggg
      541 gattcccttt cgacccgagc atccgtatga gactcatgct cgattattat tattatagaa
      601 gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc
      661 tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg
      721 gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct
      781 gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat
      841 ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt
      901 ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg
      961 gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg
     1021 cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc
     1081 gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg
     1141 ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg
     1201 cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg
     1261 ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc
     1321 gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg
     1381 gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt
     1441 tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt
     1501 ttttttggtg aatcgcattc tgactggttg cctgtcagag gcggagaatc tggtgatttt
     1561 gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt
     1621 gagctcgctg gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc
     1681 gaggtcatca actggcagga ggaacaggag ggtgcatgct tggtgataac ggcaattccg
     1741 ggagtaccgg cggctgatct gtctggagcg gatttgctca aagcgtggcc gtcaatgggg
     1801 cagcaacttg gcgctgttca cagcctatcg gttgatcaat gtccgtttga gcgcaggctg
     1861 tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc
     1921 ttaccggacg aggacaagag tacgccgcag ctcgatcttt tggctcgtgt cgaactagag
     1981 ctaccggtgc ggctcgacca agagcgcacc gttaacccag gatgagaacc ttgaaagtat
     2041 cattgatagc tgcgaaagcg aaaaacggcg tgattggttg cggtccagac ataccctggt
     2101 ccgcgaaagg ggagcagcta ctttttaaag cattgaccta caatcagtgg cttctggtgg
     2161 gtcgcaagac gtttgaatct atgggcgcac tccccaatag gaaatacgcg gtcgttaccc
     2221 gctcaggttg gacatcaaat gatgacaatg tagttgtatt tcagtcaatc gaagaggcca
     2281 tggacaggct agctgaattc accggtcacg ttatagtgtc tggtggcgga gaaatttacc
     2341 gagaaacatt acccatggcc tctacgctcc acttatcgac gatcgacatc gagccagagg
     2401 gggatgtttt cttcccgagt attccaaata ccttcgaagt tgtttttgag caacacttta
     2461 cttcaaacat taactattgc tatcaaattt ggaaaaaggg ttaacaaagc tatgcaatcg
     2521 acggcaaaaa gcttcgttcg cttcgcgcac tacgcctttt tccgcgattg atagcgacga
     2581 tatggttgtt tgccatggtg atccctgcat gccgaacttc atggtggacc ctaaaactct
     2641 tcaatgcacg ggtctgatcg accttgggcg gctcggaaca gcagatcgct atgccgattt
     2701 ggcactcatg attgctaacg ccgaagagaa ctgggcagcg ccagatgaag cagagcgcgc
     2761 cttcgctgtc ctattcaatg tattggggat cgaagccccc gaccgcgaac gccttgcctt
     2821 ctatctgcga ttggaccctc tgacttgggg ttgatgttca tgccgcctgt ttttcctgct
     2881 cattggcacg tttcgcaacc tgttctcatt gcggacacct tttccagcct cgtttggaaa
     2941 gtttcattgc cagacgggac tcctgcaatc gtcaagggat tgaaacctat agaagacatt
     3001 gctgatgaac tgcgcggggc cgactatctg gtatggcgca atgggagggg agcagtccgg
     3061 ttgctcggtc gtgagaacaa tctgatgttg ctcgaatatg ccggggagcg aatgctctct
     3121 cacatcgttg ccgagcacgg cgactaccag gcgaccgaaa ttgcagcgga actaatggcg
     3181 aagctgtatg ccgcatctga ggaacccctg ccttctgccc ttctcccgat ccgggatcgc
     3241 tttgcagctt tgtttcagcg ggcgcgcgat gatcaaaacg caggttgtca aactgactac
     3301 gtccacgcgg cgattatagc cgatcaaatg atgagcaatg cctcggaact gcgtgggcta
     3361 catggcgatc tgcatcatga aaacatcatg ttctccagtc gcggctggct ggtgatagat
     3421 cccgtcggtc tggtcggtga agtgggcttt ggcgccgcca atatgttcta cgatccggct
     3481 gacagagacg acctttgtct cgatcctaga cgcattgcac agatggcgga cgcattctct
     3541 cgtgcgctgg acgtcgatcc gcgtcgcctg ctcgaccagg cgtacgctta tgggtgcctt
     3601 tccgcagctt ggaacgcgga tggagaagag gagcaacgcg atctagctat cgcggccgcg
     3661 atcaagcagg tgcgacagac gtcatactag atatcaagcg acttctccta tcccctggga
     3721 acacatcaat ctcaccggag aatatcgctg gccaaagcct tagcgtagga ttccgcccct
     3781 tcccgcaaac gaccccaaac aggaaacgca gctgaaacgg gaagctcaac acccactgac
     3841 gcatgggttg ttcaggcagt acttcatcaa ccagcaaggc ggcactttcg gccatccgcc
     3901 gcgccccaca gctcgggcag aaaccgcgac gcttacagct gaaagcgacc aggtgctcgg
     3961 cgtggcaaga ctcgcagcga acccgtagaa agccatgctc cagccgcccg cattggagaa
     4021 attcttcaaa ttcccgttgc acatagcccg gcaattcctt tccctgctct gccataagcg
     4081 cagcgaatgc cgggtaatac tcgtcaacga tctgatagag aagggtttgc tcgggtcggt
     4141 ggctctggta acgaccagta tcccgatccc ggctggccgt cctggccgcc acatgaggca
     4201 tgttccgcgt ccttgcaata ctgtgtttac atacagtcta tcgcttagcg gaaagttctt
     4261 ttaccctcag ccgaaatgcc tgccgttgct agacattgcc agccagtgcc cgtcactccc
     4321 actcttacat tgttcgcagt atctaggtac atgttattgc gtgcaccttc atgatgtata
     4381 atgttattaa tgacagcaga aagatcgaca gaattaatat tcttcgtacc tgggacagtt
     4441 atcgggtgtc ggccagtgat gagaccaaag gatgcagtat aagatgaacc actactatta
     4501 tcatggccac cattaccacc accgttaaaa ctacctcccg aacttccaga gccgctatga
     4561 ccatcattgt aggggtcatt tggtcccatg tgaccaaaac caccgacagt accgctattt
     4621 aactctgtac tcataaaatt atcctctttg acttttaaaa acagcaaaaa ctgtatttat
     4681 aacaatgtgt ttatatacag tattcgaaat actacgcccg tcaaaaccac accgtcaaca
     4741 ggttttttgt gtatttttca cgcctcacgg gcagcctcag accttttgtg ttaaaacagt
     4801 aatatgttct gctgttacaa tacggtacac acaggagaca ctgtcacttt tatggcatgg
     4861 cacacagttc aagagacact gaaattaaca ggaaagtcgc gctcgcaatt atatcgtgac
     4921 atggctaaag gccttgtcag ctatcgcaca ggaaaaaatg atcgccggga gttcgagacc
     4981 agtgaactga ttcgggccta tggcgagctg cgcacttctg agacaccaaa aaggcacaag
     5041 gagggacact ccaaatctgt cgttagccag gcggaaaatg ctcagctaga cgtcattcag
     5101 cagaaactgg acgctctgca gcagactgtt accctaatgc ttgaagacaa gatggcgcgg
     5161 gaagcagaaa accgcctgcg aaatgaggag agggaacaac tacaggccga aattagccgt
     5221 ctggctgacg cgctggcaca ggaaaaaaag cggggattct ggtcgcggct gtttcgctcc
     5281 ggttcagagt aacagtgcag gtgactctga tttaccataa catctgttat cagcaccgcc
     5341 tgagaggccc tcagggcgtt tctgacggga aaccggtaaa acaggcaaca aaccacccga
     5401 aaacccgcca gaaacgcgca ggctctgttt ttacggcatg catgagtatg cgcacaagtg
     5461 catagcgcgg aaattcacac cattcagccg gggtaagtcc tgtttccggc gttcttccca
     5521 tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc gtgaagcgtt gccggcgcag
     5581 ccggaaaacc cgctgtcagc acgttcagga agggggtgat gtacaaatgg cacagaggat
     5641 gaaagtgacg tatgagctga aggaatgatg taaaagtgat gtaaaagtga tgtataagtg
     5701 atgtaaaagt gatgtatgaa ctgaaggagc gatgtaattc tgtagtcagg aagaataacc
     5761 cgttcatgca ggcatgaacg atacagtgag gaacgctttt tttgtcacga tctcccctta
     5821 acctgcgtaa atcacccgcc atggttgatt atttttcaac ctcaaaatgg tcatacacct
     5881 aacgcagccg cgattttcat cgcaactgca tgaattaaaa gaaaaaaaag gaatatttcg
     5941 ttttcacgag taatacccgg gtattaaaaa tccactgtaa ttacatgaaa ataaagaaat
     6001 aaaaggtaat accggagtat taccacgtac gcgggcaagc gaaaaagcta taacgcttcc
     6061 cggccgacag gcgacccaaa atccacaggg gcatgcaggt tctcttcagt aacctgcgcc
     6121 attaaatcaa aaaggtcata ttcgccggga agctgcgtat cagtcgaatc cagttgttca
     6181 tcatattcag atggcatggg gtgatatcca gtagctgaga ggttgttagc gggtttcggg
     6241 gcgcagccct gaaccagtca tgtagcgcta gcggagtgta tactggctta ctatgttgcc
     6301 acgattgcga aaatcagtga agtgcttcat gtggcaggag aaaaaaggca gcaccggcgc
     6361 gtcagcagaa tatgcgatac aggagatatt ccgcttcctc gctcactgac tcgctacgct
     6421 cggtcgtt
//
LOCUS       DAJTSL010000387         4915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.guided.222, whole genome shotgun sequence.
ACCESSION   DAJTSL010000387 DAJTSL010000000
VERSION     DAJTSL010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.guided.222"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            203..607
                     /locus_tag="OO433_004299"
     CDS             203..607
                     /locus_tag="OO433_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6052520.1"
                     /translation="MGISLNHGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQ
                     QWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSL
                     YESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(585..1208)
                     /gene="tetR(B)"
                     /locus_tag="OO433_004300"
     CDS             complement(585..1208)
                     /gene="tetR(B)"
                     /locus_tag="OO433_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6052521.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1290..2495
                     /gene="tet(B)"
                     /locus_tag="OO433_004301"
     CDS             1290..2495
                     /gene="tet(B)"
                     /locus_tag="OO433_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6052522.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2608..3201)
                     /gene="tetC"
                     /locus_tag="OO433_004302"
     CDS             complement(2608..3201)
                     /gene="tetC"
                     /locus_tag="OO433_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6052523.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3334..3705
                     /locus_tag="OO433_004303"
     CDS             3334..3705
                     /locus_tag="OO433_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6052524.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(3715..>4915)
                     /locus_tag="OO433_004304"
     CDS             complement(3715..>4915)
                     /locus_tag="OO433_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR6052525.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1323 a          930 c         1053 g         1609 t
ORIGIN      
        1 acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga gtcattaccc
       61 tgtggcgaat atatcagctt caacggcgag cagtcattta tctaaattat tagattgcca
      121 gctaatcaca gtagtagctc aaggcaagca tcgttatttt cggctagcag gaaaagatat
      181 tgctgaattg atggaaagta tgatggggat ctccttaaac catggcgtac atgccaaagt
      241 ttccacgcca gtgcatttac gaaaagcacg tacttgctat gatcatttag ctggcgaagt
      301 tgccgttaag atctatgatt ccctttgtca acagcaatgg atcactgaaa atggttcaat
      361 gatcacatta agtggtattc aatattttca tgaaatggga attgacgttc cttccaaaca
      421 ttcacgtaaa atctgttgtg cgtgtttaga ttggagtgaa cgccgtttcc atttaggtgg
      481 gtacgttgga gccgcattat tttcgcttta tgaatctaaa gggtggttaa ctcgacatct
      541 tggttaccgt gaagttacca tcacggaaaa aggttatgct gcttttaaga cccactttca
      601 catttaagtt gtttttctaa tccgcatatg atcaattcaa ggccgaataa gaaggctggc
      661 tctgcacctt ggtgatcaaa taattcgata gcttgtcgta ataatggcgg catactatca
      721 gtagtaggtg tttccctttc ttctttagcg acttgatgct cttgatcttc caatacgcaa
      781 cctaaagtaa aatgccccac agcgctgagt gcatataatg cattctctag tgaaaaacct
      841 tgttggcata aaaaggctaa ttgattttcg agagtttcat actgtttttc tgtaggccgt
      901 gtacctaaat gtacttttgc tccatcgcga tgacttagta aagcacatct aaaactttta
      961 gcgttattac gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa gtgagtatgg
     1021 tgcctatcta acatctcaat ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc
     1081 caatacaatg taggctgctc tacacctagc ttctgggcga gtttacgggt tgttaaacct
     1141 tcgattccga cctcattaag cagctctaat gcgctgttaa tcactttact tttatctaat
     1201 ctagacatca ttaattccta atttttgttg acactctatc attgatagag ttattttacc
     1261 actccctatc agtgatagag aaaagtgaaa tgaatagttc gacaaagatc gcattggtaa
     1321 ttacgttact cgatgccatg gggattggcc ttatcatgcc agtcttgcca acgttattac
     1381 gtgaatttat tgcttcggaa gatatcgcta accactttgg cgtattgctt gcactttatg
     1441 cgttaatgca ggttatcttt gctccttggc ttggaaaaat gtctgaccga tttggtcggc
     1501 gcccagtgct gttgttgtca ttaataggcg catcgctgga ttacttattg ctggcttttt
     1561 caagtgcgct ttggatgctg tatttaggcc gtttgctttc agggatcaca ggagctactg
     1621 gggctgtcgc ggcatcggtc attgccgata ccacctcagc ttctcaacgc gtgaagtggt
     1681 tcggttggtt aggggcaagt tttgggcttg gtttaatagc ggggcctatt attggtggtt
     1741 ttgcaggaga gatttcaccg catagtccct tttttatcgc tgcgttgcta aatattgtcg
     1801 ctttccttgt ggttatgttt tggttccgtg aaaccaaaaa tacacgtgat aatacagata
     1861 ccgaagtagg ggttgagacg caatcgaatt cggtatacat cactttattt aaaacgatgc
     1921 ccattttgtt gattatttat ttttcagcgc aattgatagg ccaaattccc gcaacggtgt
     1981 gggtgctatt taccgaaaat cgttttggat ggaatagcat gatggttggc ttttcattag
     2041 cgggtcttgg tcttttacac tcagtattcc aagcctttgt ggcaggaaga atagccacta
     2101 aatggggcga aaaaacggca gtactgctcg gatttattgc agatagtagt gcatttgcct
     2161 ttttagcgtt tatatctgaa ggttggttag ttttccctgt tttaatttta ttggctggtg
     2221 gtgggatcgc tttacctgca ttacagggag tgatgtctat ccaaacaaag agtcatcagc
     2281 aaggtgcttt acagggatta ttggtgagcc ttaccaatgc aaccggtgtt attggcccat
     2341 tactgtttgc tgttatttat aatcattcac taccaatttg ggatggctgg atttggatta
     2401 ttggtttagc gttttactgt attattatcc tgctatcgat gaccttcatg ttaacccctc
     2461 aagctcaggg gagtaaacag gagacaagtg cttagttatt tcgtcaccaa atgatgttat
     2521 tccgcgaaat ataatgaccc tcttgataac ccaagagggc attttttacg ataaagaaga
     2581 tttagcttca aataaaacct atctatttta tttatctttc aagctcaata aaaagccgcg
     2641 gtaaatagca ataaattggc cttttttatc ggcaagctct tttaggtttt tcgcatgtat
     2701 tgcgatatgc ataaaccagc cattgagtaa gtttttaagc acatcatcat cataagcttt
     2761 aagttggttc tcttggatca atttgctgac aatggcgttt accttaccag taatgtattc
     2821 aaggctaatt ttttcaagtt cattccaacc aatgataggc atcacttctt ggatagggat
     2881 aaggttttta ttattatcaa taatataatc aagataatgt tcaaatatac tttctaaggc
     2941 agaccaacca tttgttaaat cagtttttgt tgtgatgtag gcatcaatca taattaattg
     3001 ctgcttataa caggcactga gtaattgttt tttattttta aagtgatgat aaaaggcacc
     3061 tttggtcacc aacgcttttc ccgagatctc atctattgaa acagcttgat agcctttttc
     3121 aacaaacaat attcgtgctg agttaaccag tgattgatag gtactcttaa aattttcttg
     3181 ttgatgattt ttattttcca tgatagattt aaaataacat accgtcagta tgtttatggt
     3241 atcatgatga tgtggtcgtg acaatcttaa gaacatttag gttattttat gtatattgaa
     3301 cagcattctc gctatcaaaa taaagctaat aacatccaat taagatatga tgataagcag
     3361 tttcatacaa cggttatcaa agatgttcta ttatggattg aacataattt agatcagtct
     3421 ttactgcttg atgatgtggc gaataaagcg ggttatacca agtggtattt tcagcggctg
     3481 ttcaaaaaag taacaggggt cacactggct agctatattc gtgctcgtcg tttgacgaaa
     3541 gcggctgttg agttgaggtt gacgaaaaaa actatccttg agatcgcatt aaaatatcaa
     3601 tttgattccc aacaatcttt tacacgtcga tttaagtaca tttttaaggt tacaccaagt
     3661 tattatcggc gtaataaatt atgggaattg gaggcaatgc actgagagat cccctcataa
     3721 tttccccaaa gcgtaaccat gtgtgaataa attttgagct agtagggttg cagccacgag
     3781 taagtcttcc cttgttattg tgtagccaga atgccgcaaa acttccatgc ctaagcgaac
     3841 tgttgagagt acgtttcgat ttctgactgt gttagcctgg aagtgcttgt cccaaccttg
     3901 tttctgagca tgaacgcccg caagccaaca tgttagttga agcatcaggg cgattagcag
     3961 catgatatca aaacgctctg agctgctcgt tcggctatgg cgtaggccta gtccgtaggc
     4021 aggacttttc aagtctcgga aggtttcttc aatctgcatt cgcttcgaat agatattaac
     4081 aagttgtttg ggtgttcgaa tttcaacagg taagttagtt gctagaaccc atggctcctt
     4141 tgccgacgct gagtagattt taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg
     4201 attttttcgg cctttagagc gagatttata caatagaatt tggcatgaga ttggattgct
     4261 tttagtcagc ctcttatagc ctaaagtctt tgagtgacta gatgacatat catgtaagtt
     4321 gctgataggt ttccagtttt ccgctcctag gtctgcatat tgtacttttc ctcttactcg
     4381 acttaaccag taccaaccca gcttctcaac ggatttatac catggcactt taaagccagc
     4441 atcactgaca atgagcggtg tggtgttact cggtagaatg ctcgcaaggt cggctagaaa
     4501 ttggtcatga gctttctttg aacattgctc tgaaagcggg aacgctttct cataaagagt
     4561 aacagaacga ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt tttgctcacg
     4621 aatatcagac cagtcaacaa gtacaatggg catcgtattg cccgaacaga taaagctagc
     4681 atgccaacgg tatacagcga gtcgctcttt gtggaggtga cgattaccta acaatcggtc
     4741 gattcgtttg atgttatgtt ttgttctcgc tttggttggc aggttacggc caagttcggt
     4801 aagagtgaga gttttacagt caagtaatgc gtggcaagcc aacgttaagc tgttgagtcg
     4861 ttttaagtgt aattcggggt aatgactcca acttactgat agtgttttat gttca
//
LOCUS       DAJTSL010000388         1521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603694
            SAMEA8900739-rid15817453.guided.310, whole genome shotgun sequence.
ACCESSION   DAJTSL010000388 DAJTSL010000000
VERSION     DAJTSL010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900739
            Sequence Read Archive: ERR6005193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478107.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478107.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:42:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,897
            CDSs (with protein)               :: 3,897
            Genes (RNA)                       :: 74
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 184 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 98 of 335
            Pseudo Genes (multiple problems)  :: 37 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900739-rid15817453.guided.310"
                     /serovar="S. flexneri 6"
                     /isolate="603694"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            17..475
                     /gene="gpt"
                     /locus_tag="OO433_004305"
     CDS             17..475
                     /gene="gpt"
                     /locus_tag="OO433_004305"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6052526.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            567..>1521
                     /gene="frsA"
                     /locus_tag="OO433_004306"
     CDS             567..>1521
                     /gene="frsA"
                     /locus_tag="OO433_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6052527.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYL"
BASE COUNT          334 a          391 c          416 g          380 t
ORIGIN      
        1 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
       61 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
      121 aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
      181 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgagagctta aagtgctgaa
      241 acgcgcagaa ggcgatggcg aaggcttcat agttattgat gacctggtgg ataccggtgg
      301 tactgctgtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
      361 accggctggt cgtccgctgg ttgatgacta tgttgttgat attccgcaag atacctggat
      421 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
      481 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcaggtta caatagtttc
      541 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
      601 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
      661 aaccgcctgt gcagtcggct cttgatggta aaaccatccc tcactggtat cgcatgatta
      721 accgtctgat gtggatctgg cgcggtattg acccacgtga aatcctcgac gtccaggcac
      781 gtattgtgat gagcgatgct gaacgtaccg acgatgattt atacgatact gtgattggct
      841 accgtggtgg caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag
      901 cctgtgcgga agaagatccg caactcagtg gtcgacactg gctgcatgcg gctacgttgt
      961 acaacattgc cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt
     1021 tatcaaaccg cgcctatgaa gaggccgccc agcgtctgcc gggcacgatg cggcagatgg
     1081 agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg
     1141 gcccgttccc gacagtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca
     1201 gcctgtatga acgttatttt gcgccgcgcg gcattgtgat gctgactatt gatatgccgt
     1261 cggtgggctt ttcttcaaaa tggaagctca cccaggactc cagcctgttg catcagcacg
     1321 tcttaaaggc gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt
     1381 tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag
     1441 cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac
     1501 aggtgccgga aatgtatctt g
//
